Monkeypox Virus Host Factor Screen Using Haploid Cells Identifies Essential Role of GARP Complex in Extracellular Virus Formation.

PubMed

Realegeno, Susan; Puschnik, Andreas S; Kumar, Amrita; Goldsmith, Cynthia; Burgado, Jillybeth; Sambhara, Suryaprakash; Olson, Victoria A; Carroll, Darin; Damon, Inger; Hirata, Tetsuya; Kinoshita, Taroh; Carette, Jan E; Satheshkumar, Panayampalli Subbian

2017-06-01

Monkeypox virus (MPXV) is a human pathogen that is a member of the Orthopoxvirus genus, which includes Vaccinia virus and Variola virus (the causative agent of smallpox). Human monkeypox is considered an emerging zoonotic infectious disease. To identify host factors required for MPXV infection, we performed a genome-wide insertional mutagenesis screen in human haploid cells. The screen revealed several candidate genes, including those involved in Golgi trafficking, glycosaminoglycan biosynthesis, and glycosylphosphatidylinositol (GPI)-anchor biosynthesis. We validated the role of a set of vacuolar protein sorting (VPS) genes during infection, VPS51 to VPS54 (VPS51-54), which comprise the Golgi-associated retrograde protein (GARP) complex. The GARP complex is a tethering complex involved in retrograde transport of endosomes to the trans -Golgi apparatus. Our data demonstrate that VPS52 and VPS54 were dispensable for mature virion (MV) production but were required for extracellular virus (EV) formation. For comparison, a known antiviral compound, ST-246, was used in our experiments, demonstrating that EV titers in VPS52 and VPS54 knockout (KO) cells were comparable to levels exhibited by ST-246-treated wild-type cells. Confocal microscopy was used to examine actin tail formation, one of the viral egress mechanisms for cell-to-cell dissemination, and revealed an absence of actin tails in VPS52KO- or VPS54KO-infected cells. Further evaluation of these cells by electron microscopy demonstrated a decrease in levels of wrapped viruses (WVs) compared to those seen with the wild-type control. Collectively, our data demonstrate the role of GARP complex genes in double-membrane wrapping of MVs necessary for EV formation, implicating the host endosomal trafficking pathway in orthopoxvirus infection. IMPORTANCE Human monkeypox is an emerging zoonotic infectious disease caused by Monkeypox virus (MPXV). Of the two MPXV clades, the Congo Basin strain is associated with severe disease, increased mortality, and increased human-to-human transmission relative to the West African strain. Monkeypox is endemic in regions of western and central Africa but was introduced into the United States in 2003 from the importation of infected animals. The threat of MPXV and other orthopoxviruses is increasing due to the absence of routine smallpox vaccination leading to a higher proportion of naive populations. In this study, we have identified and validated candidate genes that are required for MPXV infection, specifically, those associated with the Golgi-associated retrograde protein (GARP) complex. Identifying host targets required for infection that prevents extracellular virus formation such as the GARP complex or the retrograde pathway can provide a potential target for antiviral therapy. Copyright © 2017 American Society for Microbiology.

Genome Sequences of Akhmeta Virus, an Early Divergent Old World Orthopoxvirus.

PubMed

Gao, Jinxin; Gigante, Crystal; Khmaladze, Ekaterine; Liu, Pengbo; Tang, Shiyuyun; Wilkins, Kimberly; Zhao, Kun; Davidson, Whitni; Nakazawa, Yoshinori; Maghlakelidze, Giorgi; Geleishvili, Marika; Kokhreidze, Maka; Carroll, Darin S; Emerson, Ginny; Li, Yu

2018-05-12

Annotated whole genome sequences of three isolates of the Akhmeta virus (AKMV), a novel species of orthopoxvirus (OPXV), isolated from the Akhmeta and Vani regions of the country Georgia, are presented and discussed. The AKMV genome is similar in genomic content and structure to that of the cowpox virus (CPXV), but a lower sequence identity was found between AKMV and Old World OPXVs than between other known species of Old World OPXVs. Phylogenetic analysis showed that AKMV diverged prior to other Old World OPXV. AKMV isolates formed a monophyletic clade in the OPXV phylogeny, yet the sequence variability between AKMV isolates was higher than between the monkeypox virus strains in the Congo basin and West Africa. An AKMV isolate from Vani contained approximately six kb sequence in the left terminal region that shared a higher similarity with CPXV than with other AKMV isolates, whereas the rest of the genome was most similar to AKMV, suggesting recombination between AKMV and CPXV in a region containing several host range and virulence genes.

Congo Basin precipitation: Assessing seasonality, regional interactions, and sources of moisture

NASA Astrophysics Data System (ADS)

Dyer, Ellen L. E.; Jones, Dylan B. A.; Nusbaumer, Jesse; Li, Harry; Collins, Owen; Vettoretti, Guido; Noone, David

2017-07-01

Precipitation in the Congo Basin was examined using a version of the National Center for Atmospheric Research Community Earth System Model (CESM) with water tagging capability. Using regionally defined water tracers, or tags, the moisture contribution from different source regions to Congo Basin precipitation was investigated. We found that the Indian Ocean and evaporation from the Congo Basin were the dominant moisture sources and that the Atlantic Ocean was a comparatively small source of moisture. In both rainy seasons the southwestern Indian Ocean contributed about 21% of the moisture, while the recycling ratio for moisture from the Congo Basin was about 25%. Near the surface, a great deal of moisture is transported from the Atlantic into the Congo Basin, but much of this moisture is recirculated back over the Atlantic in the lower troposphere. Although the southwestern Indian Ocean is a major source of Indian Ocean moisture, it is not associated with the bulk of the variability in precipitation over the Congo Basin. In wet years, more of the precipitation in the Congo Basin is derived from Indian Ocean moisture, but the spatial distribution of the dominant sources is shifted, reflecting changes in the midtropospheric circulation over the Indian Ocean. During wet years there is increased transport of moisture from the equatorial and eastern Indian Ocean. Our results suggest that reliably capturing the linkages between the large-scale circulation patterns over the Indian Ocean and the local circulation over the Congo Basin is critical for future projections of Congo Basin precipitation.

Spatial analysis from remotely sensed observations of Congo basin of East African high Land to drain water using gravity for sustainable management of low laying Chad basin of Central Africa

NASA Astrophysics Data System (ADS)

Modu, B.; Herbert, B.

2014-11-01

The Chad basin which covers an area of about 2.4 million kilometer square is one of the largest drainage basins in Africa in the centre of Lake Chad .This basin was formed as a result of rifting and drifting episode, as such it has no outlet to the oceans or seas. It contains large area of desert from the north to the west. The basin covers in part seven countries such as Chad, Nigeria, Central African Republic, Cameroun, Niger, Sudan and Algeria. It is named Chad basin because 43.9% falls in Chad republic. Since its formation, the basin continues to experienced water shortage due to the activities of Dams combination, increase in irrigations and general reduction in rainfall. Chad basin needs an external water source for it to be function at sustainable level, hence needs for exploitation of higher east African river basin called Congo basin; which covers an area of 3.7 million square km lies in an astride the equator in west-central Africa-world second largest river basin after Amazon. The Congo River almost pans around republic of Congo, the democratic republic of Congo, the Central African Republic, western Zambia, northern Angola, part of Cameroun, and Tanzania. The remotely sensed imagery analysis and observation revealed that Congo basin is on the elevation of 275 to 460 meters and the Chad basin is on elevation of 240 meters. This implies that water can be drained from Congo basin via headrace down to the Chad basin for the water sustainability.

Geology and total petroleum systems of the West-Central Coastal province (7203), West Africa

USGS Publications Warehouse

Brownfield, Michael E.; Charpentier, Ronald R.

2006-01-01

The West-Central Coastal Province of the Sub-Saharan Africa Region consists of the coastal and offshore areas of Cameroon, Equatorial Guinea, Gabon, Democratic Republic of the Congo, Republic of the Congo, Angola (including the disputed Cabinda Province), and Namibia. The area stretches from the east edge of the Niger Delta south to the Walvis Ridge. The West-Central Coastal Province includes the Douala, Kribi-Campo, Rio Muni, Gabon, Congo, Kwanza, Benguela, and Namibe Basins, which together form the Aptian salt basin of equatorial west Africa. The area has had significant exploration for petroleum; more than 295 oil fields have been discovered since 1954. Since 1995, several giant oil fields have been discovered, especially in the deep-water area of the Congo Basin. Although many total petroleum systems may exist in the West-Central Coastal Province, only four major total petroleum systems have been defined. The area of the province north of the Congo Basin contains two total petroleum systems: the Melania-Gamba Total Petroleum System, consisting of Lower Cretaceous source and reservoir rocks, and the Azile-Senonian Total Petroleum System, consisting of Albian to Turonian source rocks and Cretaceous reservoir rocks. Two assessment units are defined in the West-Central Coastal Province north of the Congo Basin: the Gabon Subsalt and the Gabon Suprasalt Assessment Units. The Congo Basin contains the Congo Delta Composite Total Petroleum System, consisting of Lower Cretaceous to Tertiary source and reservoir rocks. The Central Congo Delta and Carbonate Platform and the Central Congo Turbidites Assessment Units are defined in the Congo Delta Composite Total Petroleum System. The area south of the Congo Basin contains the Cuanza Composite Total Petroleum System, consisting of Lower Cretaceous to Tertiary source and reservoir rocks. The Cuanza-Namibe Assessment Unit is defined in the Cuanza Composite Total Petroleum System. The U.S. Geological Survey (USGS) assessed the potential for undiscovered conventional oil and gas resources in this province as part of its World Petroleum Assessment 2000. The USGS estimated a mean of 29.7 billion barrels of undiscovered conventional oil, 88.0 trillion cubic feet of gas, and 4.2 billion barrels of natural gas liquids. Most of the hydrocarbon potential remains in the offshore waters of the province in the Central Congo Turbidites Assessment Unit. Large areas of the offshore parts of the Kwanza, Douala, Kribi-Campo, and Rio Muni Basins are underexplored, considering their size, and current exploration activity suggests that the basins have hydrocarbon potential. Since about 1995, the offshore part of the Congo Basin has become a major area for new field discoveries and for hydrocarbon exploration, and many deeper water areas in the basin have excellent hydrocarbon potential. Gas resources may be significant and accessible in areas where the zone of oil generation is relatively shallow.

Hydrological trends in Congo basin (Central Africa)

NASA Astrophysics Data System (ADS)

Laraque, A.

2015-12-01

The last studies concerning some main Congo basin rivers allowed to subdivide their multi-annual flows into several homogeneous phases. As in West Africa, 1970 was the year of the major hydroclimatic event announcing a weaker flowing period. In the absence of long, reliable and available flow series in the whole Congo basin of 3,8 106km2 area, the present study concerns only the Congo River at Brazzaville/Kinshasa and two of the main tributaries of its right bank, Ubangui at Bangui and Sangha at Ouesso, with hydrologic data available from the first half of the 20th century. For Congo River, in comparison with its secular average, after an excess flow noted during the sixties, a significant drop of 10% occurs in the eighties. However, a return to normal conditions is recorded from 1995. For Ubangui and Sangha, the flows remain weaker since 1970. Within the bi-modal hydrological regimes of Sangha and Congo river, because they are equatorial, we also observe since many years a small decline of the secondary flood of april-june. This phenomenon was emphasized especially these last years and is founded in others rivers of Central Africa, where it reflects the variations of de rainfall patterns and the surfaces features. For the Congo basin, the situation is worrying because that affects the inland waterway transport. Moreover that wakes also the project of junction by a canal of the Congo and Chari basins for fighting against the hydrological decline of Lake Chad.

To Tip or Not to Tip: The Case of the Congo Basin Rainforest Realm

NASA Astrophysics Data System (ADS)

Pietsch, S.; Bednar, J. E.; Fath, B. D.; Winter, P. A.

2017-12-01

The future response of the Congo basin rainforest, the second largest tropical carbon reservoir, to climate change is still under debate. Different Climate projections exist stating increase and decrease in rainfall and different changes in rainfall patterns. Within this study we assess all options of climate change possibilities to define the climatic thresholds of Congo basin rainforest stability and assess the limiting conditions for rainforest persistence. We use field data from 199 research plots from the Western Congo basin to calibrate and validate a complex BioGeoChemistry model (BGC-MAN) and assess model performance against an array of possible future climates. Next, we analyze the reasons for the occurrence of tipping points, their spatial and temporal probability of occurrence, will present effects of hysteresis and derive probabilistic spatial-temporal resilience landscapes for the region. Additionally, we will analyze attractors of forest growth dynamics and assess common linear measures for early warning signals of sudden shifts in system dynamics for their robustness in the context of the Congo Basin case, and introduce the correlation integral as a nonlinear measure of risk assessment.

A process-based investigation into the impact of the Congo basin deforestation on surface climate

NASA Astrophysics Data System (ADS)

Bell, Jean P.; Tompkins, Adrian M.; Bouka-Biona, Clobite; Sanda, I. Seidou

2015-06-01

The sensitivity of climate to the loss of the Congo basin rainforest through changes in land cover properties is examined using a regional climate model. The complete removal of the Congo basin rainforest results in a dipole rainfall anomaly pattern, characterized by a decrease (˜-42%) in rainfall over the western Congo and an increase (˜10%) in the basin's eastern part. Three further experiments systematically examine the individual response to the changes in albedo, surface roughness, and evapotranspiration efficiency that accompany deforestation. The increased albedo (˜) caused by the Congo basin rainforest clearance results in cooler and drier climate conditions over the entire basin. The drying is accompanied with a reduction in available surface energy. Reducing evapotranspiration efficiency or roughness length produces similar positive air temperature anomaly patterns. The decreased evapotranspiration efficiency leads to a dipole response in rainfall, similar to that resulting from a reduced surface roughness following Congo basin rainforest clearance. This precipitation anomaly pattern is strongly linked to the change in low-level water vapor transport, the influence of the Rift valley highlands, and the spatial pattern of water recycling activity. The climate responds linearly to the separate albedo, surface roughness, and evapotranspiration efficiency changes, which can be summed to produce a close approximation to the impact of the full deforestation experiment. It is suggested that the widely contrasting climate responses to deforestation in the literature could be partly due to the relative magnitude of change of the radiative and nonradiative parameterizations in their respective land surface schemes.

Characterizing Congo Basin rainfall and climate using Tropical Rainfall Measuring Mission (TRMM) satellite data and limited rain gauge ground observations

USGS Publications Warehouse

Munzimi, Yolande A.; Hansen, Matthew C.; Adusei, Bernard; Senay, Gabriel B.

2015-01-01

Quantitative understanding of Congo River basin hydrological behavior is poor because of the basin’s limited hydrometeorological observation network. In cases such as the Congo basin where ground data are scarce, satellite-based estimates of rainfall, such as those from the joint NASA/JAXA Tropical Rainfall Measuring Mission (TRMM), can be used to quantify rainfall patterns. This study tests and reports the use of limited rainfall gauge data within the Democratic Republic of Congo (DRC) to recalibrate a TRMM science product (TRMM 3B42, version 6) in characterizing precipitation and climate in the Congo basin. Rainfall estimates from TRMM 3B42, version 6, are compared and adjusted using ground precipitation data from 12 DRC meteorological stations from 1998 to 2007. Adjustment is achieved on a monthly scale by using a regression-tree algorithm. The output is a new, basin-specific estimate of monthly and annual rainfall and climate types across the Congo basin. This new product and the latest version-7 TRMM 3B43 science product are validated by using an independent long-term dataset of historical isohyets. Standard errors of the estimate, root-mean-square errors, and regression coefficients r were slightly and uniformly better with the recalibration from this study when compared with the 3B43 product (mean monthly standard errors of 31 and 40 mm of precipitation and mean r2 of 0.85 and 0.82, respectively), but the 3B43 product was slightly better in terms of bias estimation (1.02 and 1.00). Despite reasonable doubts that have been expressed in studies of other tropical regions, within the Congo basin the TRMM science product (3B43) performed in a manner that is comparable to the performance of the recalibrated product that is described in this study.

The long-term evolution of the Congo deep-sea fan: A basin-wide view of the interaction between a giant submarine fan and a mature passive margin (ZaiAngo project)

NASA Astrophysics Data System (ADS)

Anka, Zahie; Séranne, Michel; Lopez, Michel; Scheck-Wenderoth, Magdalena; Savoye, Bruno

2009-05-01

We have integrated the relatively unknown distal domains of the Lower Congo basin, where the main depocenters of the Congo submarine fan are located, with the better-constrained successions on the shelf and upper slope, through the analysis of thousands of km of 2D seismic reflection profiles off-shore the Congo-Angola passive margin. The basin architecture is depicted by two ca. 800-km-long regional cross sections through the northern (Congo) and southern (Angola) margin. A large unit deposited basinward of the Aptian salt limit is likely to be the abyssal-plain equivalent of the upper-Cretaceous carbonate shelf that characterized the first post-rift deposits in West-equatorial African margins. A latest-Turonian shelf-deepening event is recorded in the abyssal plain as a long period (Coniacian-Eocene) of condensed sedimentation and basin starvation. The onset of the giant Tertiary Congo deep-sea fan in early Oligocene following this event reactivates the abyssal plain as the main depocenter of the basin. The time-space partitioning of sedimentation within the deep-sea fan results from the interplay among increasing sediment supply, margin uplift, rise of the Angola salt ridge, and canyon incision throughout the Neogene. Oligocene-early Miocene turbidite sedimentation occurs mainly in NW-SE grabens and ponded inter-diapir basins on the southern margin (Angola). Seaward tilting of the margin and downslope salt withdrawal activates the up-building of the Angola escarpment, which leads to a northward (Congo) shift of the transfer zones during late Miocene. Around the Miocene-Pliocene boundary, the incision of the Congo submarine canyon confines the turbidite flows and drives a general basinward progradation of the submarine fan into the abyssal plain The slope deposition is dominated by fine-grained hemipelagic deposits ever since. Results from this work contribute to better understand the signature in the ultra-deep deposits of processes acting on the continental margin as well as the basin-wide sediment redistribution in areas of high river input.

Labeo rosae (Cypriniformes: Cyprinidae) in the Congo basin: a relict distribution or a historical introduction?

PubMed

Van Steenberge, M; Gajdzik, L; Chilala, A; Snoeks, J; Vreven, E

2014-11-01

Labeo rosae, a species with a native range in Southern Africa, was discovered in the Congo basin by re-identification of two museum specimens previously identified as Labeo mesops. The occurrence of this species in the upper Congo implies a range extension of the species of more than 1000 km. Although the species' distribution is mirrored by that of some other Cypriniformes, its occurrence in the Congo might be due to introduction by humans. © 2014 The Fisheries Society of the British Isles.

The ecological and cultural functions of invertebrates in the Congo River basin.

Treesearch

Bruce G. Marcot

2005-01-01

One of the entomologically richest, yet least studied, regions of Africa is the interior Congo River Basin. Forests of this region have been called Earth's "second lung" (after the Amazon Basin forests) and harbor an immense diversity of invertebrates. In these tropical rainforests live people of several cultures whose lives and livelihoods are...

Intraplate compressional deformation in West-Congo and the Congo basin: related to ridge-puch from the South Atlantic spreading ridge?

NASA Astrophysics Data System (ADS)

Delvaux, Damien; Everaerts, Michel; Kongota Isasi, Elvis; Ganza Bamulezi, Gloire

2016-04-01

After the break-up and separation of South America from Africa and the initiation of the South-Atlantic mid-oceanic ridge in the Albian, at about 120 Ma, ridge-push forces started to build-up in the oceanic lithosphere and were transmitted to the adjacent continental plates. This is particularly well expressed in the passive margin and continental interior of Central Africa. According to the relations of Wiens and Stein (1985) between ridge-push forces and basal drag in function of the lithospheric age of oceanic plates, the deviatoric stress reaches a compressional maximum between 50 and 100, Ma after the initiation of the spreading ridge, so broadly corresponding to the Paleocene in this case (~70-20 Ma). Earthquake focal mechanism data show that the West-Congo margin and a large part of the Congo basin are still currently under compressional stresses with an horizontal compression parallel to the direction of the active transform fracture zones. We studied the fracture network along the Congo River in Kinshasa and Brazzaville which affect Cambrian sandstones and probably also the late Cretaceous-Paleocene sediments. Their brittle tectonic evolution is compatible with the buildup of ridge-push forces related to the South-Atlantic opening. Further inland, low-angle reverse faults are found affecting Jurassic to Middle Cretaceous cores from the Samba borehole in the Congo basin and strike-slip movements are recorded as a second brittle phase in the Permian cores of the Dekese well, at the southern margin of the Congo basin. An analysis of the topography and river network of the Congo basin show the development of low-amplitude (50-100 m) long wavelengths (100-300 km) undulations that can be interpreted as lithospheric buckling in response to the compressional intraplate stress field generated by the Mid-Atlantic ridge-push. Wiens, D.A., Stein, S., 1985. Implications of oceanic intraplate seismicity for plate stresses, driving forces and theology. Tectonophysics 1166, 143-162.

Enhancing case definitions for surveillance of human monkeypox in the Democratic Republic of Congo.

PubMed

Osadebe, Lynda; Hughes, Christine M; Shongo Lushima, Robert; Kabamba, Joelle; Nguete, Beatrice; Malekani, Jean; Pukuta, Elisabeth; Karhemere, Stomy; Muyembe Tamfum, Jean-Jacques; Wemakoy Okitolonda, Emile; Reynolds, Mary G; McCollum, Andrea M

2017-09-01

Human monkeypox (MPX) occurs at appreciable rates in the Democratic Republic of Congo (DRC). Infection with varicella zoster virus (VZV) has a similar presentation to that of MPX, and in areas where MPX is endemic these two illnesses are commonly mistaken. This study evaluated the diagnostic utility of two surveillance case definitions for MPX and specific clinical characteristics associated with laboratory-confirmed MPX cases. Data from a cohort of suspect MPX cases (identified by surveillance over the course of a 42 month period during 2009-2014) from DRC were used; real-time PCR diagnostic test results were used to establish MPX and VZV diagnoses. A total of 333 laboratory-confirmed MPX cases, 383 laboratory-confirmed VZV cases, and 36 cases that were determined to not be either MPX or VZV were included in the analyses. Significant (p<0.05) differences between laboratory-confirmed MPX and VZV cases were noted for several signs/symptoms including key rash characteristics. Both surveillance case definitions had high sensitivity and low specificities for individuals that had suspected MPX virus infections. Using 12 signs/symptoms with high sensitivity and/or specificity values, a receiver operator characteristic analysis showed that models for MPX cases that had the presence of 'fever before rash' plus at least 7 or 8 of the 12 signs/symptoms demonstrated a more balanced performance between sensitivity and specificity. Laboratory-confirmed MPX and VZV cases presented with many of the same signs and symptoms, and the analysis here emphasized the utility of including 12 specific signs/symptoms when investigating MPX cases. In order to document and detect endemic human MPX cases, a surveillance case definition with more specificity is needed for accurate case detection. In the absence of a more specific case definition, continued emphasis on confirmatory laboratory-based diagnostics is warranted.

Conventional tree height-diameter relationships significantly overestimate aboveground carbon stocks in the Central Congo Basin.

PubMed

Kearsley, Elizabeth; de Haulleville, Thales; Hufkens, Koen; Kidimbu, Alidé; Toirambe, Benjamin; Baert, Geert; Huygens, Dries; Kebede, Yodit; Defourny, Pierre; Bogaert, Jan; Beeckman, Hans; Steppe, Kathy; Boeckx, Pascal; Verbeeck, Hans

2013-01-01

Policies to reduce emissions from deforestation and forest degradation largely depend on accurate estimates of tropical forest carbon stocks. Here we present the first field-based carbon stock data for the Central Congo Basin in Yangambi, Democratic Republic of Congo. We find an average aboveground carbon stock of 162 ± 20  Mg  C  ha(-1) for intact old-growth forest, which is significantly lower than stocks recorded in the outer regions of the Congo Basin. The best available tree height-diameter relationships derived for Central Africa do not render accurate canopy height estimates for our study area. Aboveground carbon stocks would be overestimated by 24% if these inaccurate relationships were used. The studied forests have a lower stature compared with forests in the outer regions of the basin, which confirms remotely sensed patterns. Additionally, we find an average soil carbon stock of 111 ± 24  Mg  C  ha(-1), slightly influenced by the current land-use change.

Description of two new Bathyaethiops species (Teleostei: Alestidae) from the Congo basin.

PubMed

Moritz, Timo; Schliewen, Ulrich K

2016-06-02

Two new species of Bathyaethiops (Teleostei: Characiformes: Alestidae) are described. Bathyaethiops baka n. sp. is a dwarf species with the largest known specimen being only 24.4 mm SL. The species is characterized by an incomplete squamation and a large humeral spot. Bathyaethiops baka n. sp. is known so far only from the Ngoko River of Southeastern Cameroon, a tributary of the Sangha River in the northern Congo basin. The second species, Bathyaethiops flammeus n. sp., shows a diagnostic spot in front of the dorsal-fin base, which is devoid of melanophores and bright red in life. The species is described from the Bakéré River at Yambula-Bakéré, a locality north-west of Kisangani in the Central Congo basin. Other records of Bathyaethiops flammeus n. sp. from the Tshuapa respectively Ruki River at Boende and Eala, Central Congo basin, suggests a wider geographic distribution. A key to all species of Bathyaethiops is provided.

Nitrogen fluxes in the forests of the Congo Basin

NASA Astrophysics Data System (ADS)

Bauters, Marijn; Verbeeck, Hans; Cizungu, Landry; Makelele, Isaac; Boeckx, Pascal

2017-04-01

The tropical forest of the Congo basin remains very poorly investigated and understood; mainly because of logistic, political and research capacity constraints. Nevertheless, characterization and monitoring of fundamental processes in this biome is vital to understand future responses and to correctly parameterize Earth system models. Nutrient fluxes are key in these processes for the functioning of tropical forests, since CO2 uptake by terrestrial ecosystems strongly depends on site fertility, i.e. nutrient availability. Accurate projections of future net forest growth and terrestrial CO2 uptake thus necessitate an improved understanding on nutrient cycles and how these are coupled to the carbon (C) cycle in forests. Research in the Congo Basin region should combine assessments of both carbon fluxes and the underlying nutrient cycles which directly impact the forest productivity. We set up a monitoring network for nitrogen fluxes in four different forest types in the Congo Basin, resulting in a unique and integrate dataset. The questions to be answered: How do the N-budgets of four different forest types in the Congo Basin compare? How do these fluxes compare to fluxes in the Amazon forest? What is the influence from the strong slash-and-burn regimes on the N-cycle in the natural forests? We answer these questions with our empirical dataset of one hydrological year, combined with satellite and modeling data.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Le Fournier, J.

The Congo basin, in Equatorial Africa, is an enormous topographic depression, roughly coinciding with the drainage system of the Congo (or Zaire) River and its tributaries. It covers nearly 2 million sq km, mostly in the Republic of Zaire, the Republic of Congo, and the Central African Republic. With only 2,900 km of seismic profiling and two exploration wells, it can be considered almost unexplored. No exploration work is in progress. Data derived from wells, seismic profiles, and some field reconnaissance surveys have been reviewed in initial articles by Lawrence et al. and Daly et al. Based on the availablemore » data, these authors have proposed a model of the basin, which is summarized. Based on the same data complemented by more-elaborate sedimentological interpretations, focusing on paleoenvironmental reconstruction, this author proposes a significantly different model concluding in the nonexistence of the Galamboge play, at least in the central part of the basin. He counters with the alternative of the probable existence of a variety of other plays located at different stratigraphic intervals and distributed in different sectors of the basin. While this alternative model certainly downgrades the Galamboge sandstones play, in return it considerably upgrades the global estimate of the petroleum potential of the Congo basin. Indeed, it shows that this basin displays most of the characteristics generally attached to major intracratonic petroleum provinces.« less

South to south learning in great ape conservation.

PubMed

Schoneveld-de Lange, Nicolien; Meijaard, Erik; Löhr, Ansje

2016-06-01

Despite evidence that killing of Bornean Orangutan (Pongo pygmaeus) in South-East Asia is a major threat to the species, few researchers and non-governmental conservationists have addressed it in management and research, and there is virtually no implementation of anti-killing strategies. In large parts of the Congo Basin, Central Africa, instead, illegal killing of great apes is acknowledged to be their largest threat, and many conservation strategies have been used to reduce killing pressure. However, since these strategies have not been subject to systematic and comprehensive review, it remains unclear which of them have been successful and why. Knowledge of the success, failure, and practices of common conservation strategies to manage great ape killing is critical to ensure adaptive conservation management in the Congo Basin. Understanding the Congo context also facilitates simultaneously highlighting great ape killing in Borneo and suggesting solutions to manage orangutan killing. Here, we compile and analyze the available literature on great ape conservation strategies for reducing killing rates in the Congo Basin. Through a systematic literature review of 198 publications, we find that the most widely employed conservation strategies in the Congo Basin are legislation and law enforcement, protected area management, community-based conservation, alternatives to bushmeat consumption and trade, ecotourism, education, and capacity building. Despite lack of rigorous post-intervention evaluation of conservation impact, we derive several recommendations for addressing the orangutan killing issue in Borneo. A critical lesson, widely applicable to developing countries for conservationists and not limited to Congo Basin realities, is the need for rigorous post-intervention evaluations compared to pre-intervention baselines and over appropriate time frames. Am. J. Primatol. 78:669-678, 2016. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

Land Water Storage within the Congo Basin Inferred from GRACE Satellite Gravity Data

NASA Technical Reports Server (NTRS)

Crowley, John W.; Mitrovica, Jerry X.; Bailey, Richard C.; Tamisiea, Mark E.; Davis, James L.

2006-01-01

GRACE satellite gravity data is used to estimate terrestrial (surface plus ground) water storage within the Congo Basin in Africa for the period of April, 2002 - May, 2006. These estimates exhibit significant seasonal (30 +/- 6 mm of equivalent water thickness) and long-term trends, the latter yielding a total loss of approximately 280 km(exp 3) of water over the 50-month span of data. We also combine GRACE and precipitation data set (CMAP, TRMM) to explore the relative contributions of the source term to the seasonal hydrological balance within the Congo Basin. We find that the seasonal water storage tends to saturate for anomalies greater than 30-44 mm of equivalent water thickness. Furthermore, precipitation contributed roughly three times the peak water storage after anomalously rainy seasons, in early 2003 and 2005, implying an approximately 60-70% loss from runoff and evapotranspiration. Finally, a comparison of residual land water storage (monthly estimates minus best-fitting trends) in the Congo and Amazon Basins shows an anticorrelation, in agreement with the 'see-saw' variability inferred by others from runoff data.

Understanding the hydrodynamics of the Congo River

NASA Astrophysics Data System (ADS)

O'Loughlin, Fiachra; Bates, Paul

2014-05-01

We present the results of the first hydrodynamic model of the middle reach of the Congo Basin, which helps our understanding of the behaviour of the second largest river in the world. In data sparse area, hydrodynamic models, utilizing a mixture of limited in-situ measurements and remotely sensed datasets, can be used to understand and identify key features that control large river systems. Unlike previous hydrodynamic models for the Congo Basin, which concentrated on only a small area, we look at the entire length of the Congo's middle reach and its six main tributaries (Kasai, Ubangai, Sangha, Ruki, Lulonga and Lomami). This corresponds to: a drainage area of approximately two and a half million kilometres squared; over 5000 kilometres of river channels; and incorporates some of the largest and most important global wetlands. The hydrodynamic model is driven by a mixture of in-situ and modelled discharges. In situ measurements are available at five locations. Two were obtained from the Global River Discharge Centre (GRDC) at Kinshasa and Bangui, and data for Kisangani, Ouesso and Lediba were obtained from local agencies in the Democratic Republic of the Congo and the Republic of Congo. Using the gauging station at Kinshasa as the downstream boundary, the remaining in-situ measurements account for 61 percent of the discharge and represent 72 percent of the total drainage area. Modelled discharges are used to account for the missing discharge and corresponding area. Calibration and validation of the model was undertaken using a mixture of in-situ measurements, discharge and water level at Kinshasa, and water surface heights along the main reach obtained from both laser and radar altimeters. Through the hydrodynamic model we will investigate: how important constraints, identified by a previous study, are to the behaviour of the Congo; what impacts the wetlands have on the Congo Basin; how the wetlands and main channel interact with each other. Our results will provide new insight into the behaviour of the middle reach of the Congo Basin which otherwise would not be possible without extensive field work.

A proposed drainage evolution model for Central Africa—Did the Congo flow east?

NASA Astrophysics Data System (ADS)

Stankiewicz, Jacek; de Wit, Maarten J.

2006-01-01

Understanding the origin of Sub-Saharan biodiversity requires knowing the history of the region's paleo-ecosystems. As water is essential for sustaining of life, the evolving geometry of river basins often have influence on local speciation. With this in mind, we analyse drainage patterns in Central and East Africa. Evidence from marine fossils suggests the Congo Basin was submerged for much of the Cretaceous, and after being uplifted drained eastwards through a paleo-Congo river towards the Indian Ocean. Two remnant peneplains in the Congo Basin are interpreted as evidence that this basin was tectonically stable on at least two occasions in the past. The lower peneplain is interpreted as the base level of the drainage pattern that had its outlet in Tanzania, at the present Rufiji Delta that was once over 500 km wide. The Luangwa, today a tributary of the Zambezi river, was a part of this drainage network. This pattern was subsequently disrupted by uplift associated with the East African Rifting in the Oligocene-Eocene (30-40 Ma). The resulting landlocked system was captured in the Miocene (5-15 Ma) by short rivers draining into the Atlantic Ocean, producing the drainage pattern of Central Africa seen today.

42 CFR 71.56 - African rodents and other animals that may carry the monkeypox virus.

Code of Federal Regulations, 2012 CFR

2012-10-01

... the monkeypox virus. 71.56 Section 71.56 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND... and other animals that may carry the monkeypox virus. (a) What actions are prohibited? What animals... transmitting or carrying the monkeypox virus. Such products include, but are not limited to, fully taxidermied...

42 CFR 71.56 - African rodents and other animals that may carry the monkeypox virus.

Code of Federal Regulations, 2013 CFR

2013-10-01

... the monkeypox virus. 71.56 Section 71.56 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND... and other animals that may carry the monkeypox virus. (a) What actions are prohibited? What animals... transmitting or carrying the monkeypox virus. Such products include, but are not limited to, fully taxidermied...

42 CFR 71.56 - African rodents and other animals that may carry the monkeypox virus.

Code of Federal Regulations, 2011 CFR

2011-10-01

... the monkeypox virus. 71.56 Section 71.56 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND... and other animals that may carry the monkeypox virus. (a) What actions are prohibited? What animals... transmitting or carrying the monkeypox virus. Such products include, but are not limited to, fully taxidermied...

42 CFR 71.56 - African rodents and other animals that may carry the monkeypox virus.

Code of Federal Regulations, 2014 CFR

2014-10-01

... the monkeypox virus. 71.56 Section 71.56 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND... and other animals that may carry the monkeypox virus. (a) What actions are prohibited? What animals... transmitting or carrying the monkeypox virus. Such products include, but are not limited to, fully taxidermied...

42 CFR 71.56 - African rodents and other animals that may carry the monkeypox virus.

Code of Federal Regulations, 2010 CFR

2010-10-01

... the monkeypox virus. 71.56 Section 71.56 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND... and other animals that may carry the monkeypox virus. (a) What actions are prohibited? What animals... transmitting or carrying the monkeypox virus. Such products include, but are not limited to, fully taxidermied...

Hydraulic characterization of the middle reach of the Congo River

NASA Astrophysics Data System (ADS)

O'Loughlin, F.; Trigg, M.; Schumann, G.; Bates, P. D.

2012-12-01

Little is known about the hydraulics of the Congo River compared to other large rivers, such as the Amazon, Nile and Mississippi, despite it draining an area greater than 3.7 million square kilometers and being the seconded largest river in terms of discharge. While there has been some study of the Congo Basin, most of these concentrate on ecology or the human aspects, but few look at the either the hydrology or hydraulic characteristics of the river. Of the published hydrology/hydraulic research, most concentrates on the hydrology of the Congo Basin aiming to alleviate some of the issues relating to a sparse river gauging network that currently exists. Even fewer studies have looked at hydraulics of the Congo, and usually over a relatively small area of the basin. To undertake a larger study area requires more details on the characteristics of the Congo River. The Congo River can be divided into three distinct reaches; the upper, middle and lower reaches. We concentrate on the middle reach which starts upstream at Boyoma falls, just south of Kisangani, and ends downstream at Livingstone Falls, at Kinshasa (DRC), Brazzaville (Congo) and the Pool Malebo. From Kisangani to Kinshasa, the middle Congo crosses the equator twice and is join by two large tributaries (Ubangi, Kasai) and is highly braided. The middle reach of the Congo is especially important as its still largely undisturbed wetlands are the seconded largest tropical wetlands globally. It is also the main transportation link between Kisangani and Kinshasa, the two largest cities in the DRC. By utilizing remotely sensed Landsat and Icesat datasets, we present the first detailed study on the hydraulic characterization of the middle reach of the Congo River. With these datasets we identify the main control points of flow in the middle reach, investigate how the water surface slope, channel width, islands and braids vary between high and low flows and spatially along the reach. We compare the middle reach of the Congo to other large braided rivers to highlight how the Congo is unique. This detailed analysis will yield key hydraulic characteristics for large reaches of the main channel and tributaries that will be essential for correct hydraulic modeling of the river in due course, and will also provide new insights into the behavior and hydrodynamics of this mysterious river.

Recent summer precipitation trends in the Greater Horn of Africa and the emerging role of Indian Ocean sea surface temperature

NASA Astrophysics Data System (ADS)

Williams, A. Park; Funk, Chris; Michaelsen, Joel; Rauscher, Sara A.; Robertson, Iain; Wils, Tommy H. G.; Koprowski, Marcin; Eshetu, Zewdu; Loader, Neil J.

2012-11-01

We utilize a variety of climate datasets to examine impacts of two mechanisms on precipitation in the Greater Horn of Africa (GHA) during northern-hemisphere summer. First, surface-pressure gradients draw moist air toward the GHA from the tropical Atlantic Ocean and Congo Basin. Variability of the strength of these gradients strongly influences GHA precipitation totals and accounts for important phenomena such as the 1960s-1980s rainfall decline and devastating 1984 drought. Following the 1980s, precipitation variability became increasingly influenced by the southern tropical Indian Ocean (STIO) region. Within this region, increases in sea-surface temperature, evaporation, and precipitation are linked with increased exports of dry mid-tropospheric air from the STIO region toward the GHA. Convergence of dry air above the GHA reduces local convection and precipitation. It also produces a clockwise circulation response near the ground that reduces moisture transports from the Congo Basin. Because precipitation originating in the Congo Basin has a unique isotopic signature, records of moisture transports from the Congo Basin may be preserved in the isotopic composition of annual tree rings in the Ethiopian Highlands. A negative trend in tree-ring oxygen-18 during the past half century suggests a decline in the proportion of precipitation originating from the Congo Basin. This trend may not be part of a natural cycle that will soon rebound because climate models characterize Indian Ocean warming as a principal signature of greenhouse-gas induced climate change. We therefore expect surface warming in the STIO region to continue to negatively impact GHA precipitation during northern-hemisphere summer.

Susceptibility of Marmosets (Callithrix jacchus) to Monkeypox Virus: A Low Dose Prospective Model for Monkeypox and Smallpox Disease.

PubMed

Mucker, Eric M; Chapman, Jennifer; Huzella, Louis M; Huggins, John W; Shamblin, Joshua; Robinson, Camenzind G; Hensley, Lisa E

2015-01-01

Although current nonhuman primate models of monkeypox and smallpox diseases provide some insight into disease pathogenesis, they require a high titer inoculum, use an unnatural route of infection, and/or do not accurately represent the entire disease course. This is a concern when developing smallpox and/or monkeypox countermeasures or trying to understand host pathogen relationships. In our studies, we altered half of the test system by using a New World nonhuman primate host, the common marmoset. Based on dose finding studies, we found that marmosets are susceptible to monkeypox virus infection, produce a high viremia, and have pathological features consistent with smallpox and monkeypox in humans. The low dose (48 plaque forming units) required to elicit a uniformly lethal disease and the extended incubation (preclinical signs) are unique features among nonhuman primate models utilizing monkeypox virus. The uniform lethality, hemorrhagic rash, high viremia, decrease in platelets, pathology, and abbreviated acute phase are reflective of early-type hemorrhagic smallpox.

Susceptibility of Marmosets (Callithrix jacchus) to Monkeypox Virus: A Low Dose Prospective Model for Monkeypox and Smallpox Disease

PubMed Central

Mucker, Eric M.; Chapman, Jennifer; Huzella, Louis M.; Huggins, John W.; Shamblin, Joshua; Robinson, Camenzind G.; Hensley, Lisa E.

2015-01-01

Although current nonhuman primate models of monkeypox and smallpox diseases provide some insight into disease pathogenesis, they require a high titer inoculum, use an unnatural route of infection, and/or do not accurately represent the entire disease course. This is a concern when developing smallpox and/or monkeypox countermeasures or trying to understand host pathogen relationships. In our studies, we altered half of the test system by using a New World nonhuman primate host, the common marmoset. Based on dose finding studies, we found that marmosets are susceptible to monkeypox virus infection, produce a high viremia, and have pathological features consistent with smallpox and monkeypox in humans. The low dose (48 plaque forming units) required to elicit a uniformly lethal disease and the extended incubation (preclinical signs) are unique features among nonhuman primate models utilizing monkeypox virus. The uniform lethality, hemorrhagic rash, high viremia, decrease in platelets, pathology, and abbreviated acute phase are reflective of early-type hemorrhagic smallpox. PMID:26147658

Taxonomic challenges in freshwater fishes: a mismatch between morphology and DNA barcoding in fish of the north-eastern part of the Congo basin.

PubMed

Decru, Eva; Moelants, Tuur; De Gelas, Koen; Vreven, Emmanuel; Verheyen, Erik; Snoeks, Jos

2016-01-01

This study evaluates the utility of DNA barcoding to traditional morphology-based species identifications for the fish fauna of the north-eastern Congo basin. We compared DNA sequences (COI) of 821 samples from 206 morphologically identified species. Best match, best close match and all species barcoding analyses resulted in a rather low identification success of 87.5%, 84.5% and 64.1%, respectively. The ratio 'nearest-neighbour distance/maximum intraspecific divergence' was lower than 1 for 26.1% of the samples, indicating possible taxonomic problems. In ten genera, belonging to six families, the number of species inferred from mtDNA data exceeded the number of species identified using morphological features; and in four cases indications of possible synonymy were detected. Finally, the DNA barcodes confirmed previously known identification problems within certain genera of the Clariidae, Cyprinidae and Mormyridae. Our results underscore the large number of taxonomic problems lingering in the taxonomy of the fish fauna of the Congo basin and illustrate why DNA barcodes will contribute to future efforts to compile a reliable taxonomic inventory of the Congo basin fish fauna. Therefore, the obtained barcodes were deposited in the reference barcode library of the Barcode of Life Initiative. © 2015 John Wiley & Sons Ltd.

The nitrogen budget for different forest types in the central Congo Basin

NASA Astrophysics Data System (ADS)

Bauters, Marijn; Verbeeck, Hans; Cizungu, Landry; Boeckx, Pascal

2016-04-01

Characterization of fundamental processes in different forest types is vital to understand the interaction of forests with their changing environment. Recent data analyses, as well as modeling activities have shown that the CO2 uptake by terrestrial ecosystems strongly depends on site fertility, i.e. nutrient availability. Accurate projections of future net forest growth and terrestrial CO2 uptake thus necessitate an improved understanding on nutrient cycles and how these are coupled to the carbon (C) cycle in forests. This holds especially for tropical forests, since they represent about 40-50% of the total carbon that is stored in terrestrial vegetation, with the Amazon basin and the Congo basin being the largest two contiguous blocks. However, due to political instability and reduced accessibility in the central Africa region, there is a strong bias in scientific research towards the Amazon basin. Consequently, central African forests are poorly characterized and their role in global change interactions shows distinct knowledge gaps, which is important bottleneck for all efforts to further optimize Earth system models explicitly including this region. Research in the Congo Basin region should combine assessments of both carbon stocks and the underlying nutrient cycles which directly impact the forest productivity. We set up a monitoring network for carbon stocks and nitrogen fluxes in four different forest types in the Congo Basin, which is now operative. With the preliminary data, we can get a glimpse of the differences in nitrogen budget and biogeochemistry of African mixed lowland rainforest, monodominant lowland forest, mixed montane forest and eucalypt plantations.

Linking plant functional traits and forest carbon stocks in the Congo Basin

NASA Astrophysics Data System (ADS)

Kearsley, Elizabeth; Verbeeck, Hans; Hufkens, Koen; Lewis, Simon; Huygens, Dries; Beeckman, Hans; Steppe, Kathy; Boeckx, Pascal

2013-04-01

Accurate estimates of the amount of carbon stored in tropical forests represent crucial baseline data for recent climate change mitigation policies. Such data are needed to quantify possible emissions due to deforestation and forest degradation, and to evaluate the potential of these forests to act as carbon sinks. Currently, only rough estimates of the carbon stocks for Central African tropical forests are available due to a lack of field data, and little is known about the response of these stocks to climate change. We present the first ground-based carbon stock data for the central Congo Basin in Yangambi, D. R. Congo, based on data of 20 inventory plots of 1 ha covering different forest types. We found an average aboveground carbon stock of 163 ± 19 Mg C ha-1 for intact old-growth forest, which is significantly lower than the stocks recorded in the outer regions of the Congo Basin. Commonly studied drivers for variations of carbon stocks include climatic and edaphic factors, but detailed trait-based studies are lacking. We identified a significant difference in height-diameter relations across the Congo Basin as a driver for spatial differences in carbon stocks. The study of a more detailed interaction of the environment and the available tree species pool as drivers for differences in carbon storage could have large implications. The effect of the species pool on carbon storage can be large since species differ in their ability to sequester carbon, and the collective functional characteristics of plant communities could be a major driver of carbon accumulation. The use of a trait-based approach shows high potential for identifying and quantifying carbon stocks as an ecosystem service. We test for associations between functional trait values and carbon storage across multiple regrowth and old-growth forests types in the Yangambi study area, with soil properties and climate similar for all plots. A selection of traits associated with carbon dynamics is made, including leaf traits (specific leaf area, leaf dry matter content, leaf carbon concentration, leaf nitrogen and phosphorus concentration, 13C, 15N, 18O), stem traits (wood density, vessel diameter), and vegetative traits (tree height, light tolerance), studying 990 individuals covering 111 species. To develop a more coherent understanding of ecosystem functioning, the related carbon sequestration, and the contribution of climate and soil factors in tropical forests in the Congo Basin, we present a preliminary spatial study of plant functional traits and diversity by comparing several sites across the Congo Basin.

Opportunities for hydrologic research in the Congo Basin

NASA Astrophysics Data System (ADS)

Alsdorf, Douglas; Beighley, Ed; Laraque, Alain; Lee, Hyongki; Tshimanga, Raphael; O'Loughlin, Fiachra; Mahé, Gil; Dinga, Bienvenu; Moukandi, Guy; Spencer, Robert G. M.

2016-06-01

We review the published results on the Congo Basin hydrology and summarize the historic and ongoing research. Annual rainfall is ~1900 mm/yr along an east-west trend across the basin, decreasing northward and southward to ~1100 mm/yr. Historic studies using lysimeters, pans, and models suggest that the annual potential evapotranspiration varies little across the basin at 1100 to 1200 mm/yr. Over the past century, river discharge data have been collected at hundreds of stream gauges with historic and recent data at 96 locations now publicly available. Congo River discharge at Kinshasa-Brazzaville experienced an increase of 21% during the 1960-1970 decade in comparison to most other decades. Satellite altimetry measurements of high and low flows show that water levels in the "Cuvette Centrale" wetland are 0.5 m to 3.0 m higher in elevation than the immediately adjacent Congo River levels. Wetland water depths are shallow at about a meter and there does not appear to be many sizable channels across the "Cuvette"; thus, wetland flows are diffusive. Cuvette waters alone are estimated to emit about 0.5 Pg CH4 and CO2 equivalents/yr, an amount that is significant compared to global carbon evasions. Using these results, we suggest seven hypotheses that focus on the source of the Cuvette waters and how these leave the wetland, on the river discharge generated by historic rainfall, on the connection between climate change and the rainfall-runoff generated by the migrating "tropical rainbelt," on deforestation and hydroelectric power generation, and on the amount of carbon emitted from Congo waters.

Opportunities for Hydrologic Research in the Congo Basin

NASA Astrophysics Data System (ADS)

Alsdorf, D. E.; Beighley, E.; Laraque, A.; Lee, H.; Tshimanga, R.; O'Loughlin, F.; Mahe, G. M.; Dinga, B. J.; Moukandi, G.; Spencer, R.

2016-12-01

We review the published results on the Congo Basin hydrology and summarize the historic and ongoing research. Annual rainfall is 1900 mm/yr along an east-west trend across the basin, decreasing northward and southward to 1100 mm/yr. Historic studies using lysimeters, pans, and models suggest that the annual potential evapotranspiration varies little across the basin at 1100 to 1200 mm/yr. Over the past century, river discharge data have been collected at hundreds of stream gauges with historic and recent data at 96 locations now publicly available. Congo River discharge at Kinshasa-Brazzaville experienced an increase of 21% during the 1960-1970 decade in comparison to most other decades. Satellite altimetry measurements of high and low flows show that water levels in the "Cuvette Centrale" wetland are 0.5m to 3.0m higher in elevation than the immediately adjacent Congo River levels. Wetland water depths are shallow at about a meter and there does not appear to be many sizable channels across the "Cuvette"; thus, wetland flows are diffusive. Cuvette waters alone are estimated to emit about 0.5 Pg CH4 and CO2 equivalents/yr, an amount that is significant compared to global carbon evasions. Using these results, we suggest seven hypotheses that focus on the source of the Cuvette waters and how these leave the wetland, on the river discharge generated by historic rainfall, on the connection between climate change and the rainfall-runoff generated by the migrating "tropical rainbelt," on deforestation and hydroelectric power generation, and on the amount of carbon emitted from Congo waters.

Organic carbon accumulation in modern sediments of the Angola basin influenced by the Congo deep-sea fan

NASA Astrophysics Data System (ADS)

Baudin, François; Martinez, Philippe; Dennielou, Bernard; Charlier, Karine; Marsset, Tania; Droz, Laurence; Rabouille, Christophe

2017-08-01

Geochemical data (total organic carbon-TOC content, δ13Corg, C:N, Rock-Eval analyses) were obtained on 150 core tops from the Angola basin, with a special focus on the Congo deep-sea fan. Combined with the previously published data, the resulting dataset (322 stations) shows a good spatial and bathymetric representativeness. TOC content and δ13Corg maps of the Angola basin were generated using this enhanced dataset. The main difference in our map with previously published ones is the high terrestrial organic matter content observed downslope along the active turbidite channel of the Congo deep-sea fan till the distal lobe complex near 5000 m of water-depth. Interpretation of downslope trends in TOC content and organic matter composition indicates that lateral particle transport by turbidity currents is the primary mechanism controlling supply and burial of organic matter in the bathypelagic depths.

Monkeypox virus and insights into its immunomodulatory proteins

PubMed Central

Weaver, Jessica R.; Isaacs, Stuart N.

2008-01-01

Summary Monkeypox is a disease that is endemic in Central and Western Africa. However, in 2003, there was an outbreak in the US, representing the first documented monkeypox cases in the Western hemisphere. Although monkeypox virus is less fatal and not as transmissible as variola virus, the causative agent of smallpox, there is concern that monkeypox virus could become a more efficient human pathogen. The reason for this may lie in the virus' genetic makeup, ecological changes, changes in host behavior, and the fact that with the eradication of variola virus, routine smallpox vaccination is no longer carried out. In this review, we focus on the viral proteins that are predicted to modulate the host immune response and compare the genome of monkeypox virus with the genomes of variola virus and the vaccinia virus, the orthopoxvirus that represented the smallpox vaccine. There are differences found in several of these immune-modulating genes including genes that express proteins that affect cytokines such as interleukin-1, tumor necrosis factor, and interferon. There are also differences in genes that code for virulence factors and host range proteins. Genetic differences likely also explain the differences in virulence between two strains of monkeypox virus found in two different regions of Africa. In the current setting of limited smallpox vaccination and little orthopoxvirus immunity in parts of the world, monkeypox could become a more efficient human pathogen under the right circumstances. PMID:18837778

Coupling between tectonics and surface processes in the Congo Basin: Cretaceous-Cenozoic sedimentation and erosion triggered by climatic and tectonic factors

NASA Astrophysics Data System (ADS)

de Putter, Thierry; Mees, Florias; Bayon, Germain; Ruffet, Gilles; Smith, Thierry; Delvaux, Damien

2017-04-01

Cretaceous to Recent evolution of the Congo Basin in Central Africa is still poorly documented although its history over the last 75 Myr has potentially recorded global and major regional events, including the Paleocene-Eocene Thermal Maximum at 56 Ma and the Miocene aperture of the Western branch of the East African Rift System along its eastern border at 25 Ma. Available data for associated off-shore deposits show that in parallel, the Congo River delta experienced a starvation period during the Mid- to Late Cretaceous and Paleogene, with endorheic lacustrine to desert environments in the upstream basin, followed by a period marked by high rates of drainage and sediment supply in the Neogene. Here, we combine new observations on the recent tectonic evolution with newly obtained 39Ar-40Ar ages for cryptomelane from Katanga (Kasekelesa) and Kasaï (Mt Mwatshimwa) and the preliminary results of the Landana condensed section ( 45 m) Paleogene-Neogene sequence. The maximum burial in the Congo Basin is estimated at 80 Ma and was followed by the removal of at least 900-1500 m of sediments (Sachse et al., 2012). Soon after the 39Ar-40Ar ages reveal that a major (Campanian or older) surface formed in the Kasai and Katanga before 76 Ma, followed by at least two younger Eocene denudation episodes, during the Lutetian ( 45 Ma) and the Priabonian ( 35 Ma) and more Mio-Pliocene denudation surfaces during the Mio-Pliocene (De Putter et al., 2016). The older surface likely belongs to the subcontinental 'African Surface' that had previously not been identified for Central Africa. During this long-lasting erosional history of the central part of the Congo Basin, the Landana section along the Atlantic coast recorded a condensed ( 45 m) sequence of Paleogene-Neogene sediments. The first 25m are shallow marine carbonates with little detrital input, recording slightly increasing weathering from the Danian to the Lutetian (Bayon et al., 2016). Whether this section had a physical connection with the inland basin at the time is not known. Simultaneously, a 150 m thick eolian sand accumulation (Kalahari Group s.l.) is assumed to have been deposited in the south-western margin of the Congo Basin. The strong silicification at the top of the Lutetian beds of the Landana section indicates a major discontinuity, which would correspond to the Lutetian denudation surface in Katanga. After this hiatus, sedimentation recorded by the Landana section changes sharply to coarse-grained siliciclastics, through a likely (re-established?) connection with the inland basin. A major change in sediment source is confirmed by ɛNd, whereas isotopic proxies of weathering (ɛHf, 30Si) document a major decrease in weathering intensity. The sharp increase in sediment discharge is attributed to uplift along the southern and eastern margins of the Congo Basin, preceding the opening of the East African Rift in the Oligocene. Bayon et al., 2016. Goldschmidt Conf. 2016, abstract book, 181 De Putter et al., 2015. Ore Geol. Rev. 71, 350-362 Sachse, V.F, Delvaux, D. and Littke, R., 2012. AAPG bulletin, 96(2), 277-300.

3D stratigraphic modeling of the Congo turbidite system since 210 ka: an investigation of factors controlling sedimentation

NASA Astrophysics Data System (ADS)

Laurent, Dimitri; Picot, Marie; Marsset, Tania; Droz, Laurence; Rabineau, Marina; Granjeon, Didier; Molliex, Stéphane

2017-04-01

The geometry and internal functioning of turbidite systems are relatively well-constrained today. However, the respective role of autogenic (topographic compensation, dynamics of turbidity currents…) and allogenic factors (tectonics, sea-level, climate) governing their architectural evolution is still under debate. The geometry of the Quaternary Congo Fan is characterized by successive sedimentary prograding/retrograding cycles bounded by upfan avulsions, reflecting a periodic control of sedimentation (Picot et al., 2016). Multi-proxy studies revealed a strong interplay between autogenic control and climate forcing as evidenced by changes in fluvial sediment supplies consistent with arid and humid periods in the Congo River Basin. In the light of these results, the aim of this study is to investigate the relative impact of internal and external forcing factors controlling, both in time and space, the formation and evolution of depocenters of the Congo Deep-Sea Fan since 210 ka. This work represents the first attempt to model in 3D the stratigraphic architecture of the Congo turbidite system using DionisosFlow (IFP-EN), a diffusion process-based software. It allows the simulation of sediment transport and the 3D geometry reproduction of sedimentary units based on physical processes such as sea level changes, tectonics, sediment supply and transport. According to the modeling results, the role of topographic compensation in the deep-sea fan geometry is secondary compared to climate changes in the drainage basin. It appears that a periodic variation of sediment discharge and water flow is necessary to simulate the timing and volume of prograding/retrograding sedimentary cycles and more particularly the upfan avulsion events. The best-fit simulations show that the overriding factor for such changes corresponds to the expansion of the vegetation cover in the catchment basin associated to the Milankovitch cycle of precession which controlled the West African Monsoon intensity. These external forcing factors are responsible for the evolution of the capacity of turbidity currents by directly acting on the river runoff magnitude and the sediment budget according to the balance between mechanical and chemical erosion. If the sediment supply is the key parameter for the large scale sedimentary cycles, a steep increase of the sand/mud ratio leads to the development of sub-cycles characterized by middle fan avulsions. We identified these events as related to abrupt destabilizations of river mouth bars linked to periodic Congo River floods. Finally, the local slope gradient only plays a role in the maximal length of the turbidity currents and deposition in the most distal part of the basin. To conclude, the stratigraphic modeling allows us to propose an evolutionary "source to sink" model of the Quaternary Congo Fan, emphasizing the interconnection through time between drainage basin responses to climate change and sedimentary transfers in the deep-water environment. Picot, M. et al., 2016. Controls on turbidite sedimentation: Insights from a quantitative approach of submarine channel and lobe architecture (Late Quaternary Congo Fan). Marine and Petroleum Geology, 72, 423-446. Keywords: Congo, sedimentary basin, Quaternary, turbidite system, sedimentary cycles, geophysical data, stratigraphic modeling, DionisosFlow

Characterization of Terrestrial Water Dynamics in the Congo Basin Using GRACE and Satellite Radar Altimetry

NASA Technical Reports Server (NTRS)

Lee, Hyongki; Beighley, R. Edward; Alsdorf, Douglas; Jung, Hahn Chul; Shum, C. K.; Duan, Jianbin; Guo, Junyi; Yamazaki, Dai; Andreadis, Konstantinos

2011-01-01

The Congo Basin is the world's third largest in size (approx.3.7 million sq km), and second only to the Amazon River in discharge (approx.40,200 cu m/s annual average). However, the hydrological dynamics of seasonally flooded wetlands and floodplains remains poorly quantified. Here, we separate the Congo wetland into four 3deg 3deg regions, and use remote sensing measurements (i.e., GRACE, satellite radar altimeter, GPCP, JERS-1, SRTM, and MODIS) to estimate the amounts of water filling and draining from the Congo wetland, and to determine the source of the water. We find that the amount of water annually filling and draining the Congo wetlands is 111 cu km, which is about one-third the size of the water volumes found on the mainstem Amazon floodplain. Based on amplitude comparisons among the water volume changes and timing comparisons among their fluxes, we conclude that the local upland runoff is the main source of the Congo wetland water, not the fluvial process of river-floodplain water exchange as in the Amazon. Our hydraulic analysis using altimeter measurements also supports our conclusion by demonstrating that water surface elevations in the wetlands are consistently higher than the adjacent river water levels. Our research highlights differences in the hydrology and hydrodynamics between the Congo wetland and the mainstem Amazon floodplain.

Characterization of Terrestrial Water Dynamics in the Congo Basin Using GRACE and Satellite Radar Altimetry

NASA Technical Reports Server (NTRS)

Lee, Lyongki; Beighley, R. Edward; Alsdorf, Douglas; Jung, Hahn Chul; Shum, C. K.; Duan, Jianbin; Guo, Junyi; Yamazaki, Dai; Andreadis, Konstantinos

2011-01-01

The Congo Basin is the world's third largest in size (approximately 3.7 million km^2), and second only to the Amazon River in discharge (approximately 40,200 cms annual average). However, the hydrological dynamics of seasonally flooded wetlands and floodplains remains poorly quantified. Here, we separate the Congo wetland into four 3 degree x 3 degree regions, and use remote sensing measurements (i.e., GRACE, satellite radar altimeter, GPCP, JERS-1, SRTM, and MODIS) to estimate the amounts of water filling and draining from the Congo wetland, and to determine the source of the water. We find that the amount of water annually filling and draining the Congo wetlands is 111 km^3, which is about one-third the size of the water volumes found on the mainstem Amazon floodplain. Based on amplitude comparisons among the water volume changes and timing comparisons among their fluxes, we conclude that the local upland runoff is the main source of the Congo wetland water, not the fluvial process of river-floodplain water exchange as in the Amazon. Our hydraulic analysis using altimeter measurements also supports our conclusion by demonstrating that water surface elevations in the wetlands are consistently higher than the adjacent river water levels. Our research also highlights differences in the hydrology and hydrodynamics between the Congo wetland and the mainstem Amazon floodplain.

Roadless Wilderness Area Determines Forest Elephant Movements in the Congo Basin

PubMed Central

Blake, Stephen; Deem, Sharon L.; Strindberg, Samantha; Maisels, Fiona; Momont, Ludovic; Isia, Inogwabini-Bila; Douglas-Hamilton, Iain; Karesh, William B.; Kock, Michael D.

2008-01-01

A dramatic expansion of road building is underway in the Congo Basin fuelled by private enterprise, international aid, and government aspirations. Among the great wilderness areas on earth, the Congo Basin is outstanding for its high biodiversity, particularly mobile megafauna including forest elephants (Loxodonta africana cyclotis). The abundance of many mammal species in the Basin increases with distance from roads due to hunting pressure, but the impacts of road proliferation on the movements of individuals are unknown. We investigated the ranging behaviour of forest elephants in relation to roads and roadless wilderness by fitting GPS telemetry collars onto a sample of 28 forest elephants living in six priority conservation areas. We show that the size of roadless wilderness is a strong determinant of home range size in this species. Though our study sites included the largest wilderness areas in central African forests, none of 4 home range metrics we calculated, including core area, tended toward an asymptote with increasing wilderness size, suggesting that uninhibited ranging in forest elephants no longer exists. Furthermore we show that roads outside protected areas which are not protected from hunting are a formidable barrier to movement while roads inside protected areas are not. Only 1 elephant from our sample crossed an unprotected road. During crossings her mean speed increased 14-fold compared to normal movements. Forest elephants are increasingly confined and constrained by roads across the Congo Basin which is reducing effective habitat availability and isolating populations, significantly threatening long term conservation efforts. If the current road development trajectory continues, forest wildernesses and the forest elephants they contain will collapse. PMID:18958284

Congo Basin rainfall climatology: can we believe the climate models?

PubMed

Washington, Richard; James, Rachel; Pearce, Helen; Pokam, Wilfried M; Moufouma-Okia, Wilfran

2013-01-01

The Congo Basin is one of three key convective regions on the planet which, during the transition seasons, dominates global tropical rainfall. There is little agreement as to the distribution and quantity of rainfall across the basin with datasets differing by an order of magnitude in some seasons. The location of maximum rainfall is in the far eastern sector of the basin in some datasets but the far western edge of the basin in others during March to May. There is no consistent pattern to this rainfall distribution in satellite or model datasets. Resolving these differences is difficult without ground-based data. Moisture flux nevertheless emerges as a useful variable with which to study these differences. Climate models with weak (strong) or even divergent moisture flux over the basin are dry (wet). The paper suggests an approach, via a targeted field campaign, for generating useful climate information with which to confront rainfall products and climate models.

Inference of R 0 and Transmission Heterogeneity from the Size Distribution of Stuttering Chains

PubMed Central

Blumberg, Seth; Lloyd-Smith, James O.

2013-01-01

For many infectious disease processes such as emerging zoonoses and vaccine-preventable diseases, and infections occur as self-limited stuttering transmission chains. A mechanistic understanding of transmission is essential for characterizing the risk of emerging diseases and monitoring spatio-temporal dynamics. Thus methods for inferring and the degree of heterogeneity in transmission from stuttering chain data have important applications in disease surveillance and management. Previous researchers have used chain size distributions to infer , but estimation of the degree of individual-level variation in infectiousness (as quantified by the dispersion parameter, ) has typically required contact tracing data. Utilizing branching process theory along with a negative binomial offspring distribution, we demonstrate how maximum likelihood estimation can be applied to chain size data to infer both and the dispersion parameter that characterizes heterogeneity. While the maximum likelihood value for is a simple function of the average chain size, the associated confidence intervals are dependent on the inferred degree of transmission heterogeneity. As demonstrated for monkeypox data from the Democratic Republic of Congo, this impacts when a statistically significant change in is detectable. In addition, by allowing for superspreading events, inference of shifts the threshold above which a transmission chain should be considered anomalously large for a given value of (thus reducing the probability of false alarms about pathogen adaptation). Our analysis of monkeypox also clarifies the various ways that imperfect observation can impact inference of transmission parameters, and highlights the need to quantitatively evaluate whether observation is likely to significantly bias results. PMID:23658504

Opportunities for Hydrologic Research in the Congo Basin

NASA Astrophysics Data System (ADS)

Alsdorf, D. E.; Beighley, E., II; Lee, H.; Tshimanga, R.; Spencer, R. G.; O'Loughlin, F.

2014-12-01

We review the published results on the Congo Basin hydrology and find that there are historic data, ongoing measurement recording efforts, and important model results. Annual rainfall is ~2000 mm/yr along an east-west trend, decreasing northward and southward to ~1100 mm/yr. While some studies show rain gauges at specific locations with declines in P greater than 10% from 1960 to 1990, other studies suggest that basin wide decreases from 1951 to 1993 are modest at 4.5% or that the trend is minimal. Studies during the 1950s using lysimeters, pans, and models suggest that the annual potential ET varies little across the basin at a 1100 mm/yr to 1200 mm/yr. Over the past century, river discharge data has been collected at 100s of stream gauges with historic and recent data at 96 locations now publicly available. Discharge of the Congo River at Kinshasa-Brazzaville experienced an increase of 21% during 1960-1970 in comparison to background values of the previous decades and of today. There does not appear to be a long-term discharge trend over the century of record. Satellite altimetry measurements collected during high and low flows show that the Cuvette Centrale wetland water levels are consistently 0.5m to 3m higher in elevation than the immediately adjacent Congo River levels. Wetland water depths are shallow at about 1m whereas the Congo is typically less than 15m deep everywhere upstream of Kinshasa. The wetlands do not appear to be marked by sizable channels such that the flows are diffusive. CO2 and CH4 evasion from the Congo waters directly to the atmosphere are estimated at 1.6 to 3.2 Tg/yr for CH4 from the Cuvette wetland waters and 105 to 204 g C/m2/yr for CO2 from waters of the Oubangui River. Using these published results, we suggest seven hypotheses that may lead to important water and carbon cycle discoveries. These hypotheses focus on the source of the Cuvette waters and how those waters leave the wetland; on river discharge generated by historic rainfall; on the connection between climate change and the rainfall-runoff generated by the ITCZ; on deforestation and hydroelectric power generation; and on the amount of carbon emitted from Congo waters. To address these hypotheses, the Congo research community will need to work together to host meetings, share ideas, access data, ensure funding, and provide infrastructural support.

High Resolution Modelling of the Congo River's Multi-Threaded Main Stem Hydraulics

NASA Astrophysics Data System (ADS)

Carr, A. B.; Trigg, M.; Tshimanga, R.; Neal, J. C.; Borman, D.; Smith, M. W.; Bola, G.; Kabuya, P.; Mushie, C. A.; Tschumbu, C. L.

2017-12-01

We present the results of a summer 2017 field campaign by members of the Congo River users Hydraulics and Morphology (CRuHM) project, and a subsequent reach-scale hydraulic modelling study on the Congo's main stem. Sonar bathymetry, ADCP transects, and water surface elevation data have been collected along the Congo's heavily multi-threaded middle reach, which exhibits complex in-channel hydraulic processes that are not well understood. To model the entire basin's hydrodynamics, these in-channel hydraulic processes must be parameterised since it is not computationally feasible to represent them explicitly. Furthermore, recent research suggests that relative to other large global rivers, in-channel flows on the Congo represent a relatively large proportion of total flow through the river-floodplain system. We therefore regard sufficient representation of in-channel hydraulic processes as a Congo River hydrodynamic research priority. To enable explicit representation of in-channel hydraulics, we develop a reach-scale (70 km), high resolution hydraulic model. Simulation of flow through individual channel threads provides new information on flow depths and velocities, and will be used to inform the parameterisation of a broader basin-scale hydrodynamic model. The basin-scale model will ultimately be used to investigate floodplain fluxes, flood wave attenuation, and the impact of future hydrological change scenarios on basin hydrodynamics. This presentation will focus on the methodology we use to develop a reach-scale bathymetric DEM. The bathymetry of only a small proportion of channel threads can realistically be captured, necessitating some estimation of the bathymetry of channels not surveyed. We explore different approaches to this bathymetry estimation, and the extent to which it influences hydraulic model predictions. The CRuHM project is a consortium comprising the Universities of Kinshasa, Rhodes, Dar es Salaam, Bristol, and Leeds, and is funded by Royal Society-DFID Africa Capacity Building Initiative. The project aims to strengthen institutional research capacity and advance our understanding of the hydrology, hydrodynamics and sediment dynamics of the world's second largest river system through fieldwork and development of numerical models.

Investigating the sources and sinks of water of Congo's wetlands

NASA Astrophysics Data System (ADS)

Paiva, R. C. D.; O'Loughlin, F.; Alsdorf, D. E.; Durand, M. T.; Beighley, E., II; Calmant, S.; Lee, H.; Santos Da Silva, J.

2014-12-01

The Congo is the second largest river basin in the world and indeed there is still a lot to be investigated about the hydrology of this system. This region presents extensive wetlands that may play an important role on the hydrology, carbon and ecological dynamics of the Congo. However, previous studies indicate that these wetlands behave differently from the Amazon, other major rainforest basin, and how water enters and leaves the Cuvette Centrale wetland is still to be quantified. We investigate the sources and sinks of water to the Congo's wetlands. Our analyses range from simple examinations of precipitation and evaporation historical data to remote sensing datasets and 2 D hydrodynamic modelling of Congo wetlands. Early results show that water levels at wetlands are usually higher than adjacent Congo River water levels and amplitude of variation is considerably smaller. Also, floodplain channels are not observed in this region indicating that surface flows are diffusive. Mean annual precipitation range from 1600 to 2000 mm/year, evapotranspiration estimates are approximately 1100 mm/year while some estimates of groundwater recharge indicate values larger than 300 mm/year. These assessments suggest that volumes coming from local water balance could flood the wetlands to depths of only a few centimeters. Preliminary 2D hydrodynamic simulations show that water coming from main rivers produces at upstream areas can flood only a small part of wetland, mainly alongside these rivers.

Congo Basin rainfall climatology: can we believe the climate models?

PubMed Central

Washington, Richard; James, Rachel; Pearce, Helen; Pokam, Wilfried M.; Moufouma-Okia, Wilfran

2013-01-01

The Congo Basin is one of three key convective regions on the planet which, during the transition seasons, dominates global tropical rainfall. There is little agreement as to the distribution and quantity of rainfall across the basin with datasets differing by an order of magnitude in some seasons. The location of maximum rainfall is in the far eastern sector of the basin in some datasets but the far western edge of the basin in others during March to May. There is no consistent pattern to this rainfall distribution in satellite or model datasets. Resolving these differences is difficult without ground-based data. Moisture flux nevertheless emerges as a useful variable with which to study these differences. Climate models with weak (strong) or even divergent moisture flux over the basin are dry (wet). The paper suggests an approach, via a targeted field campaign, for generating useful climate information with which to confront rainfall products and climate models. PMID:23878328

Geological timing and duration of methane seepage in different sedimentary and tectonic settings in the Lower Congo Basin

NASA Astrophysics Data System (ADS)

Wenau, S.; Spiess, V.

2016-12-01

Methane seepage sites have been investigated in the Lower Congo Basin using seismo-acoustic methods in combination with geological and geochemical sampling. Pockmarks were observed in different areas of the Lower Congo Basin that are affected by different styles of salt-tectonic deformation and sedimentary input. At the salt front in the southern part of the basin, methane seepage shifts continuously westwards as previously undeformed sediments are affected by westward moving salt. Older seepage sites to the East are cut off from methane supply in the process of continuing salt-tectonic deformation. The initiation of gas accumulation and seepage directly at the deformation front is expected in the late Miocene due to salt-induced uplift. In the northern part of the basin on the lower slope, methane seepage is focused along salt-tectonic faults connecting Pliocene fan deposits to the seafloor, breaching the hemipelagic seal. These sites show indications for continuing seepage for the last 640 kyrs. Such long term seepage activity may be due to the lack of polygonal faults in the hemipelagic seal, focusing gas migration on fewer, salt-tectonic faults. Westward of the salt front, seepage features include the Regab pockmark where a potential reservoir in an Early Pleistocene channel flank is connected to the seafloor feature via a seismic chimney. Seepage activity in this area is also documented to be continuous over geologic time scales by seafloor and sub-seafloor seepage indications such as chimneys, pockmarks and buried seepage features. The Lower Congo Basin thus documents the longevity of seepage processes in the context of various tectonic and sedimentary regimes on a passive continental margin. Indications of the duration of seepage activity at individual sites may be used for methane budgeting in combination with emission rates estimated for typical seepage sites.

The Upper Jurassic Stanleyville Group of the eastern Congo Basin: An example of perennial lacustrine system

NASA Astrophysics Data System (ADS)

Caillaud, Alexis; Blanpied, Christian; Delvaux, Damien

2017-08-01

The intracratonic Congo Basin, located in the Democratic Republic of Congo (DRC), is the largest sedimentary basin of Africa. The Jurassic strata outcrop along its eastern margin, south of Kisangani (formerly Stanleyville). In the last century, the Upper Jurassic Stanleyville Group was described as a lacustrine series containing a thin basal marine limestone designed as the ;Lime Fine; beds. Since the proposal of this early model, the depositional environment of the Stanleyville Group, and especially the possible marine incursion, has been debated, but without re-examining the existing cores, outcrop samples and historical fossils from the type location near Kisangani that are available at the Royal Museum for Central Africa (MRAC/KMMA, Tervuren, Belgium). In order to refine the former sedimentology, a series of nine exploration cores drilled in the Kisangani sub-basin have been described. This study aims at integrating sedimentary facies in existing sedimentary models and to discuss the hypothesis of the presence of Kimmeridgian marine deposits along the Congo River near Kisangani, a region which lies in the middle of the African continent. Eight facies have been identified, which permit a reinterpretation of the depositional environment and paleogeography of the Stanleyville Group. The base of the Stanleyville Group is interpreted to represent a conglomeratic fluvial succession, which filled an inherited Triassic paleotopography. Above these conglomerates, a transition to a typically lacustrine system is interpreted, which includes: (1) a basal profundal, sublittoral (brown to dark fine-grained siltstones with microbial carbonates, i.e., the ;Lime Fine; beds) and littoral lacustrine series; covered by (2) a sublittoral to profundal interval (brown to dark organic-rich, fine-grained siltstones), which corresponds to the maximum extent of the paleo-lake; and, finally (3) a shallow lacustrine series (greenish calcareous siltstones and sandstones with red siltstones). Unlike what has been proposed, the ;Lime Fine; beds are interpreted herein to be of lacustrine origin, rather than representing a Kimmeridgian marine transgression. We conclude that a Jurassic marine transgression did not, in fact, occur in the eastern region of the Congo Basin.

Forest Elephant Crisis in the Congo Basin

PubMed Central

Blake, Stephen; Strindberg, Samantha; Boudjan, Patrick; Makombo, Calixte; Bila-Isia, Inogwabini; Ilambu, Omari; Grossmann, Falk; Bene-Bene, Lambert; de Semboli, Bruno; Mbenzo, Valentin; S'hwa, Dino; Bayogo, Rosine; Williamson, Liz; Fay, Mike; Hart, John; Maisels, Fiona

2007-01-01

Debate over repealing the ivory trade ban dominates conferences of the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). Resolving this controversy requires accurate estimates of elephant population trends and rates of illegal killing. Most African savannah elephant populations are well known; however, the status of forest elephants, perhaps a distinct species, in the vast Congo Basin is unclear. We assessed population status and incidence of poaching from line-transect and reconnaissance surveys conducted on foot in sites throughout the Congo Basin. Results indicate that the abundance and range of forest elephants are threatened from poaching that is most intense close to roads. The probability of elephant presence increased with distance to roads, whereas that of human signs declined. At all distances from roads, the probability of elephant occurrence was always higher inside, compared to outside, protected areas, whereas that of humans was always lower. Inside protected areas, forest elephant density was correlated with the size of remote forest core, but not with size of protected area. Forest elephants must be prioritised in elephant management planning at the continental scale. PMID:17407383

Forest elephant crisis in the Congo Basin.

PubMed

Blake, Stephen; Strindberg, Samantha; Boudjan, Patrick; Makombo, Calixte; Bila-Isia, Inogwabini; Ilambu, Omari; Grossmann, Falk; Bene-Bene, Lambert; de Semboli, Bruno; Mbenzo, Valentin; S'hwa, Dino; Bayogo, Rosine; Williamson, Liz; Fay, Mike; Hart, John; Maisels, Fiona

2007-04-01

Debate over repealing the ivory trade ban dominates conferences of the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). Resolving this controversy requires accurate estimates of elephant population trends and rates of illegal killing. Most African savannah elephant populations are well known; however, the status of forest elephants, perhaps a distinct species, in the vast Congo Basin is unclear. We assessed population status and incidence of poaching from line-transect and reconnaissance surveys conducted on foot in sites throughout the Congo Basin. Results indicate that the abundance and range of forest elephants are threatened from poaching that is most intense close to roads. The probability of elephant presence increased with distance to roads, whereas that of human signs declined. At all distances from roads, the probability of elephant occurrence was always higher inside, compared to outside, protected areas, whereas that of humans was always lower. Inside protected areas, forest elephant density was correlated with the size of remote forest core, but not with size of protected area. Forest elephants must be prioritised in elephant management planning at the continental scale.

"Congo" red: out of Africa?

PubMed

Steensma, D P

2001-02-01

Congo red is the essential histologic stain for demonstrating the presence of amyloidosis in fixed tissues. To the best of my knowledge, nothing has been written about why the stain is named "Congo." To understand the etymology and history of the Congo red histologic stain. Primary sources were consulted extensively, including 19th-century corporate documents, newspapers, legal briefs, patents, memoirs, and scientific papers. Sources were obtained from multiple university libraries and German corporate archives. To Europeans in 1885, the word Congo evoked exotic images of far-off central Africa known as The Dark Continent. The African Congo was also a political flashpoint during the Age of Colonialism. "Congo" red was introduced in Berlin in 1885 as the first of the economically lucrative direct textile dyes. A patent on Congo red was filed by the AGFA Corporation of Berlin 3 weeks after the conclusion of the well-publicized Berlin West Africa Conference. During these important diplomatic talks, German Chancellor Otto von Bismarck presided over a discussion of free trade issues in the Congo River basin. A challenge to AGFA's Congo red patent led to a precedent-setting decision in intellectual property law. The Congo red stain was named "Congo" for marketing purposes by a German textile dyestuff company in 1885, reflecting geopolitical current events of that time.

Diachronous evolution of volcano-sedimentary basins north of the Congo craton: Insights from U Pb ion microprobe dating of zircons from the Poli, Lom and Yaoundé Groups (Cameroon)

NASA Astrophysics Data System (ADS)

Toteu, Sadrack Félix; Penaye, Joseph; Deloule, Etienne; Van Schmus, William Randall; Tchameni, Rigobert

2006-04-01

Ion microprobe U-Pb dating of zircons from Neoproterozoic volcano-sedimentary sequences in Cameroon north of the Congo craton is presented. For the Poli basin, the depositional age is constrained between 700-665 Ma; detrital sources comprise ca. 920, 830, 780 and 736 Ma magmatic zircons. In the Lom basin, the depositional age is constrained between 613 and 600 Ma, and detrital sources include Archaean to Palaeoproterozoic, late Mesoproterozoic to early Neoproterozoic (1100-950 Ma), and Neoproterozoic (735, 644 and 613 Ma) zircons. The Yaoundé Group is probably younger than 625 Ma, and detrital sources include Palaeoproterozoic and Neoproterozoic zircons. The depositional age of the Mahan metavolcano-sedimentary sequence is post-820 Ma, and detrital sources include late Mesoproterozoic (1070 Ma) and early Neoproterozoic volcanic rocks (824 Ma). The following conclusions can be made from these data. (1) The three basins evolved during the Pan-African event but are significantly different in age and tectonic setting; the Poli is a pre- to syn-collisional basin developed upon, or in the vicinity of young magmatic arcs; the Lom basin is post-collisional and intracontinental and developed on old crust; the tectono-metamorphic evolution of the Yaoundé Group resulted from rapid tectonic burial and subsequent collision between the Congo craton and the Adamawa-Yade block. (2) Late Mesoproterozoic to early Neoproterozoic inheritance reflects the presence of magmatic event(s) of this age in west-central Africa.

Spatial and seasonal dynamics of total suspended sediment and organic carbon species in the Congo River

NASA Astrophysics Data System (ADS)

Coynel, Alexandra; Seyler, Patrick; Etcheber, Henri; Meybeck, Michel; Orange, Didier

2005-12-01

The Congo (Zaire) River, the world's second largest river in terms both of water discharges and of drainage area after the Amazon River, has remained to date in a near-pristine state. For a period between 2 and 6 years, the mainstream near the river mouth (Brazzaville/Kinshasa station) and some of the major and minor tributaries (the Oubangui, Mpoko, and Ngoko-Sangha) were monitored every month for total suspended sediment (TSS), particulate organic carbon (POC), and dissolved organic carbon (DOC). In this large but relatively flat equatorial basin, TSS levels are very low and organic carbon is essentially exported as DOC: from 74% of TOC for the tributaries flowing in savannah regions and 86% for those flowing in the rain forest. The seasonal patterns of TSS, POC, and DOC show clockwise hysteresis in relation to river discharges, with maximum levels recorded 2 to 4 months before peak flows. At the Kinshasa/Brazzaville station, the DOC distribution is largely influenced by the input from the tributaries draining the large marshy forest area located in the center of the basin. There is a marked difference between specific fluxes, threefold higher in the forest basins than in the savannah basins. The computation of inputs to the Atlantic Ocean demonstrates that the Congo is responsible for 14.4 × 106 t/yr of TOC of which 12.4 × 106 t/yr is DOC and 2 × 106 t/yr is POC. The three biggest tropical rivers (the Amazon, the Congo, and the Orinoco), with only 10% of the exoreic world area drained to world oceans, contribute ˜4% of its TSS inputs but 15-18% of its organic carbon inputs. These proportions may double when considering only world rivers discharging into the open ocean.

Financing and current capacity for REDD+ readiness and monitoring, measurement, reporting and verification in the Congo Basin

PubMed Central

Maniatis, Danae; Gaugris, Jérôme; Mollicone, Danilo; Scriven, Joel; Corblin, Alexis; Ndikumagenge, Cleto; Aquino, André; Crete, Philippe; Sanz-Sanchez, Maria-José

2013-01-01

This paper provides the first critical analysis of the financing and current capacity for REDD+ readiness in the Congo Basin, with a particular focus on the REDD+ component of national forest monitoring and measurement, reporting and verification (M&MRV). We focus on three areas of analysis: (i) general financing for REDD+ readiness especially M&MRV; (ii) capacity and information for REDD+ implementation and M&MRV; (iii) prospects and challenges for REDD+ and M&MRV readiness in terms of financing and capacity. For the first area of analysis, a REDD+ and M&MRV readiness financing database was created based on the information from the REDD+ voluntary database and Internet searches. For the second area of analysis, a qualitative approach to data collection was adopted (semi-structured interviews with key stakeholders, surveys and observations). All 10 countries were visited between 2010 and 2012. We find that: (i) a significant amount of REDD+ financing flows into the Congo Basin (±US$550 million or almost half of the REDD+ financing for the African continent); (ii) across countries, there is an important disequilibrium in terms of REDD+ and M&MRV readiness financing, political engagement, comprehension and capacity, which also appears to be a key barrier to countries receiving equal resources; (iii) most financing appears to go to smaller scale (subnational) REDD+ projects; (iv) four distinct country groups in terms of REDD+ readiness and M&MRV status are identified; and (v) the Congo Basin has a distinct opportunity to have a specific REDD+ financing window for large-scale and more targeted national REDD+ programmes through a specific fund for the region. PMID:23878337

Financing and current capacity for REDD+ readiness and monitoring, measurement, reporting and verification in the Congo Basin.

PubMed

Maniatis, Danae; Gaugris, Jérôme; Mollicone, Danilo; Scriven, Joel; Corblin, Alexis; Ndikumagenge, Cleto; Aquino, André; Crete, Philippe; Sanz-Sanchez, Maria-José

2013-01-01

This paper provides the first critical analysis of the financing and current capacity for REDD+ readiness in the Congo Basin, with a particular focus on the REDD+ component of national forest monitoring and measurement, reporting and verification (M&MRV). We focus on three areas of analysis: (i) general financing for REDD+ readiness especially M&MRV; (ii) capacity and information for REDD+ implementation and M&MRV; (iii) prospects and challenges for REDD+ and M&MRV readiness in terms of financing and capacity. For the first area of analysis, a REDD+ and M&MRV readiness financing database was created based on the information from the REDD+ voluntary database and Internet searches. For the second area of analysis, a qualitative approach to data collection was adopted (semi-structured interviews with key stakeholders, surveys and observations). All 10 countries were visited between 2010 and 2012. We find that: (i) a significant amount of REDD+ financing flows into the Congo Basin (±US$550 million or almost half of the REDD+ financing for the African continent); (ii) across countries, there is an important disequilibrium in terms of REDD+ and M&MRV readiness financing, political engagement, comprehension and capacity, which also appears to be a key barrier to countries receiving equal resources; (iii) most financing appears to go to smaller scale (subnational) REDD+ projects; (iv) four distinct country groups in terms of REDD+ readiness and M&MRV status are identified; and (v) the Congo Basin has a distinct opportunity to have a specific REDD+ financing window for large-scale and more targeted national REDD+ programmes through a specific fund for the region.

Lac Télé structure, Republic of Congo: Geological setting of a cryptozoological and biodiversity hotspot, and evidence against an impact origin

NASA Astrophysics Data System (ADS)

Master, Sharad

2010-11-01

Lac Télé is a large lake, ˜5.6 km in diameter, with an ovoid shape, situated at 17°10'E, 1°20'N, in the great tropical rain forest region of the Republic of Congo. This lake has attracted widespread attention, mainly because of the legends among the local people that it harbours a strange animal known as the Mokele-Mbembe, but also because it is situated in a region that is a hotbed of biodiversity and conservation efforts with respect to various endangered mammalian species, including gorillas and chimpanzees. Because of its appearance, Lac Télé has been regarded as a possible meteorite impact structure. Various expeditions, studying cryptozoology, conservation ecology, biodiversity, and the impact hypothesis, have visited Lac Télé in the past several decades. The Lac Télé structure is located in the NW part of the intracratonic Congo Basin, in a region dominated by Holocene alluvium, dense tropical rain forest, and swamps which form part of the basin of the Likouala aux Herbes, a multi-branched meandering river flowing over very low gradients into the Sangha river, a major tributary of the Congo river. Previous bathymetric studies have shown that the average depth of Lac Télé is only 4 m, including organic-rich silty sediments. The structure is that of a flat-bottomed dish. Modelling of the Lac Télé as an impact structure indicates a number of features which ought to be present. The absence of any of these features, coupled with the irregular ovoid shape, the palynological record, and the location of the structure at the intersection of major regional lineaments, is regarded as evidence against the impact hypothesis. Lac Télé as an isolated lake ecosystem is not unique in the Congo Basin, and there are several other similar small shallow isolated lakes surrounded by rain forest and marshes, some of which formed by damming of drainage systems by neotectonic faults. It is suggested that the formation of Lac Télé may be related to its location over neotectonically reactivated regional lineaments, which are also seismically active. Lac Télé and other similar hydrologic systems may be biodiversity hotspots because they acted as refugia following neotectonic hydrological re-organization of the Congo Basin.

Satellite-based estimates of surface water dynamics in the Congo River Basin

NASA Astrophysics Data System (ADS)

Becker, M.; Papa, F.; Frappart, F.; Alsdorf, D.; Calmant, S.; da Silva, J. Santos; Prigent, C.; Seyler, F.

2018-04-01

In the Congo River Basin (CRB), due to the lack of contemporary in situ observations, there is a limited understanding of the large-scale variability of its present-day hydrologic components and their link with climate. In this context, remote sensing observations provide a unique opportunity to better characterize those dynamics. Analyzing the Global Inundation Extent Multi-Satellite (GIEMS) time series, we first show that surface water extent (SWE) exhibits marked seasonal patterns, well distributed along the major rivers and their tributaries, and with two annual maxima located: i) in the lakes region of the Lwalaba sub-basin and ii) in the "Cuvette Centrale", including Tumba and Mai-Ndombe Lakes. At an interannual time scale, we show that SWE variability is influenced by ENSO and the Indian Ocean dipole events. We then estimate water level maps and surface water storage (SWS) in floodplains, lakes, rivers and wetlands of the CRB, over the period 2003-2007, using a multi-satellite approach, which combines the GIEMS dataset with the water level measurements derived from the ENVISAT altimeter heights. The mean annual variation in SWS in the CRB is 81 ± 24 km3 and contributes to 19 ± 5% of the annual variations of GRACE-derived terrestrial water storage (33 ± 7% in the Middle Congo). It represents also ∼6 ± 2% of the annual water volume that flows from the Congo River into the Atlantic Ocean.

A generalized land-use scenario generator: a case study for the Congo basin.

NASA Astrophysics Data System (ADS)

Caporaso, Luca; Tompkins, Adrian Mark; Biondi, Riccardo; Bell, Jean Pierre

2014-05-01

The impact of deforestation on climate is often studied using highly idealized "instant deforestation" experiments due to the lack of generalized deforestation scenario generators coupled to climate model land-surface schemes. A new deforestation scenario generator has been therefore developed to fulfill this role known as the deforestation ScenArio GEnerator, or FOREST-SAGE. The model produces distributed maps of deforestation rates that account for local factors such as proximity to transport networks, distance weighted population density, forest fragmentation and presence of protected areas and logging concessions. The integrated deforestation risk is scaled to give the deforestation rate as specified by macro-region scenarios such as "business as usual" or "increased protection legislation" which are a function of future time. FOREST-SAGE was initialized and validated using the MODerate Resolution Imaging Spectroradiometer Vegetation Continuous Field data. Despite the high cloud coverage of Congo Basin over the year, we were able to validate the results with high confidence from 2001 to 2010 in a large forested area. Furthermore a set of scenarios has been used to provide a range of possible pathways for the evolution of land-use change over the Congo Basin for the period 2010-2030.

Terrain Analysis Procedural Guide for Climate,

DTIC Science & Technology

1980-09-01

such as the Amazon Tropical Rain Forest Basin and the Congo Basin, the air is IN Singapore F always hot and damp, there are fre-30...to protect certain mele-annual evaporation rate. These lands are orological instruments from exposure tocharacterized by rainforest , jungle, and

Early cretaceous rift sediments of the Gabon-Congo margin: lithology and organic matter; tectonic and paleogeothermal evolution

NASA Astrophysics Data System (ADS)

Robert, P.; Yapaudjian, L.

The active troughs of the western Gabon-Congo margin which are part of the South Atlantic rift contain a Neocomian to barremian-aged fluvial-lacustrine series. The lithological sequence of interbedded clastic and pelitic formations constitutes a well-defined cycle. This cycle is divided into: a fluvial or piedmont stage, a lacustrine turbidite-stage corresponding to the distension paroxysm of the basin, and finally, a lacustrine deltaic stage of infilling and tectonic quiescence. The organic matter included in the shale layers is abundant and originates mainly from lacustrine Botryococcus algae and their alteration and secretion products. The geothermal history of the basin, demonstrated by the evolution of the organic matter indicates a strong hyperthermy located in the active, more subsiding part of the basin, and contemporaneous with sedimentation.

Swimming Pools and Molluscum Contagiosum

MedlinePlus

... Monkeypox Orf Virus (Sore Mouth Infection) Poxvirus and Rabies Branch Travelers’ Health: Smallpox & Other Orthopoxvirus-Associated Infections ... Monkeypox Orf Virus (Sore Mouth Infection) Poxvirus and Rabies Branch Travelers’ Health: Smallpox & Other Orthopoxvirus-Associated Infections ...

Investigating monkeypox in the Wild

USGS Publications Warehouse

Brand, Christopher J.; Slota, Paul

2003-01-01

A recent monkeypox outbreak in pet prairie dogs led to the first recorded human case of the disease in the U.S. The outbreak has USGS scientists concerned the disease may spread to wild rodent populations.

Ecology and geography of human monkeypox case occurrences across Africa.

PubMed

Ellis, Christine K; Carroll, Darin S; Lash, Ryan R; Peterson, A Townsend; Damon, Inger K; Malekani, Jean; Formenty, Pierre

2012-04-01

As ecologic niche modeling (ENM) evolves as a tool in spatial epidemiology and public health, selection of the most appropriate and informative environmental data sets becomes increasingly important. Here, we build on a previous ENM analysis of the potential distribution of human monkeypox in Africa by refining georeferencing criteria and using more-diverse environmental data to identify environmental parameters contributing to monkeypox distributional ecology. Significant environmental variables include annual precipitation, several temperature-related variables, primary productivity, evapotranspiration, soil moisture, and pH. The potential distribution identified with this set of variables was broader than that identified in previous analyses but does not include areas recently found to hold monkeypox in southern Sudan. Our results emphasize the importance of selecting the most appropriate and informative environmental data sets for ENM analyses in pathogen transmission mapping.

Sequence of pathogenic events in cynomolgus macaques infected with aerosolized monkeypox virus.

PubMed

Tree, J A; Hall, G; Pearson, G; Rayner, E; Graham, V A; Steeds, K; Bewley, K R; Hatch, G J; Dennis, M; Taylor, I; Roberts, A D; Funnell, S G P; Vipond, J

2015-04-01

To evaluate new vaccines when human efficacy studies are not possible, the FDA's "Animal Rule" requires well-characterized models of infection. Thus, in the present study, the early pathogenic events of monkeypox infection in nonhuman primates, a surrogate for variola virus infection, were characterized. Cynomolgus macaques were exposed to aerosolized monkeypox virus (10(5) PFU). Clinical observations, viral loads, immune responses, and pathological changes were examined on days 2, 4, 6, 8, 10, and 12 postchallenge. Viral DNA (vDNA) was detected in the lungs on day 2 postchallenge, and viral antigen was detected, by immunostaining, in the epithelium of bronchi, bronchioles, and alveolar walls. Lesions comprised rare foci of dysplastic and sloughed cells in respiratory bronchioles. By day 4, vDNA was detected in the throat, tonsil, and spleen, and monkeypox antigen was detected in the lung, hilar and submandibular lymph nodes, spleen, and colon. Lung lesions comprised focal epithelial necrosis and inflammation. Body temperature peaked on day 6, pox lesions appeared on the skin, and lesions, with positive immunostaining, were present in the lung, tonsil, spleen, lymph nodes, and colon. By day 8, vDNA was present in 9/13 tissues. Blood concentrations of interleukin 1ra (IL-1ra), IL-6, and gamma interferon (IFN-γ) increased markedly. By day 10, circulating IgG antibody concentrations increased, and on day 12, animals showed early signs of recovery. These results define early events occurring in an inhalational macaque monkeypox infection model, supporting its use as a surrogate model for human smallpox. Bioterrorism poses a major threat to public health, as the deliberate release of infectious agents, such smallpox or a related virus, monkeypox, would have catastrophic consequences. The development and testing of new medical countermeasures, e.g., vaccines, are thus priorities; however, tests for efficacy in humans cannot be performed because it would be unethical and field trials are not feasible. To overcome this, the FDA may grant marketing approval of a new product based upon the "Animal Rule," in which interventions are tested for efficacy in well-characterized animal models. Monkeypox virus infection of nonhuman primates (NHPs) presents a potential surrogate disease model for smallpox. Previously, the later stages of monkeypox infection were defined, but the early course of infection remains unstudied. Here, the early pathogenic events of inhalational monkeypox infection in NHPs were characterized, and the results support the use of this surrogate model for testing human smallpox interventions. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

Sequence of Pathogenic Events in Cynomolgus Macaques Infected with Aerosolized Monkeypox Virus

PubMed Central

Hall, G.; Pearson, G.; Rayner, E.; Graham, V. A.; Steeds, K.; Bewley, K. R.; Hatch, G. J.; Dennis, M.; Taylor, I.; Roberts, A. D.; Funnell, S. G. P.; Vipond, J.

2015-01-01

ABSTRACT To evaluate new vaccines when human efficacy studies are not possible, the FDA's “Animal Rule” requires well-characterized models of infection. Thus, in the present study, the early pathogenic events of monkeypox infection in nonhuman primates, a surrogate for variola virus infection, were characterized. Cynomolgus macaques were exposed to aerosolized monkeypox virus (105 PFU). Clinical observations, viral loads, immune responses, and pathological changes were examined on days 2, 4, 6, 8, 10, and 12 postchallenge. Viral DNA (vDNA) was detected in the lungs on day 2 postchallenge, and viral antigen was detected, by immunostaining, in the epithelium of bronchi, bronchioles, and alveolar walls. Lesions comprised rare foci of dysplastic and sloughed cells in respiratory bronchioles. By day 4, vDNA was detected in the throat, tonsil, and spleen, and monkeypox antigen was detected in the lung, hilar and submandibular lymph nodes, spleen, and colon. Lung lesions comprised focal epithelial necrosis and inflammation. Body temperature peaked on day 6, pox lesions appeared on the skin, and lesions, with positive immunostaining, were present in the lung, tonsil, spleen, lymph nodes, and colon. By day 8, vDNA was present in 9/13 tissues. Blood concentrations of interleukin 1ra (IL-1ra), IL-6, and gamma interferon (IFN-γ) increased markedly. By day 10, circulating IgG antibody concentrations increased, and on day 12, animals showed early signs of recovery. These results define early events occurring in an inhalational macaque monkeypox infection model, supporting its use as a surrogate model for human smallpox. IMPORTANCE Bioterrorism poses a major threat to public health, as the deliberate release of infectious agents, such smallpox or a related virus, monkeypox, would have catastrophic consequences. The development and testing of new medical countermeasures, e.g., vaccines, are thus priorities; however, tests for efficacy in humans cannot be performed because it would be unethical and field trials are not feasible. To overcome this, the FDA may grant marketing approval of a new product based upon the “Animal Rule,” in which interventions are tested for efficacy in well-characterized animal models. Monkeypox virus infection of nonhuman primates (NHPs) presents a potential surrogate disease model for smallpox. Previously, the later stages of monkeypox infection were defined, but the early course of infection remains unstudied. Here, the early pathogenic events of inhalational monkeypox infection in NHPs were characterized, and the results support the use of this surrogate model for testing human smallpox interventions. PMID:25653439

Documenting a decline in boreal spring rainfall over the Congo Basin and eastern Africa

NASA Astrophysics Data System (ADS)

Nicholson, S. E.

2016-12-01

The Africa rain forest is one of the continent's greatest resources. Unfortunately, evidence shows that a serious decline in photosynthetic activity has occurred in widespread parts of this environmental sensitive region. An extended period of drought appears to be a major factor in this decline. This paper presents an analysis of rainfall conditions in the region over the last half century. Commencing in the 1980s April-May-June rainfall decreased markedly over the eastern Congo Basin, as well as throughout much of eastern Africa. Ironically, in areas with the greatest decline, October-November-December rainfall increased at the same time. Possible meteorological reasons for these changes are examined.

Seasonal dynamics and Organic Carbon Flux in the Congo River

NASA Astrophysics Data System (ADS)

Seyler, P.; Coynel, A.; Etcheber, H.; Meybeck, M.

2006-12-01

The Congo (Zaire) River, the second world river in terms of discharges and drainage area (Q=40600 m3/s; A=3.5 106 km2) after the Amazon River, is -up to now- in near-pristine state. For up to two years , the mainstream near river mouth (Kinshasa/Brazzaville station) and some major and minor tributaries (Oubangui, Mpoko and Ngoko-Sangha) were surveyed every month, for total suspended sediment (TSS), particulate organic carbon (POC) and dissolved organic carbon (DOC). In this very flat basin, TSS levels were very low and organic carbon was essentially exported as DOC: 74% of TOC for the tributaries flowing in savannah regions to 86% for those flowing in the rainforest). The seasonal patterns of TSS, POC and DOC showed clockwise hysteresis with river discharges, with maximum levels two to four months before peak flows. At the Kinshasa/Brazzaville station, the DOC distribution is largely influenced by the input of the tributaries draining the marshy forest area (Central depression). In term of fluxes, a marked difference is pointed out between specific fluxes, threefold higher in the forested basin than in savannahs basins. Computation of inputs to Atlantic Ocean showed that the Congo was responsible for 14.4 106 t/yr of TOC of which 12.4 106 t/yr is DOC and 2 106 t/yr is POC. The three biggest tropical rivers (Amazon, Congo and Orinoco) with only 10 percent of the exoreic world area drained to ocean world contribute to 4 percent of its TSS inputs but 29-33 percent of its organic carbon inputs.

Characteristics and geological significance of Re-Os isotopic system of evaporites in Mboukoumassi deposit, the Republic of Congo

NASA Astrophysics Data System (ADS)

Zhao, Xianfu; Wang, Zongqi; Liu, Chenglin; Li, Chao; Jiao, Pengcheng; Zhao, Yanjun; Zhang, Fan

2018-02-01

Evaporite dating has been an open problem. The study investigates the Re-Os isotopic system in the organic-rich sedimentary rocks to constrain the infilling of sedimentary basin and related geological events. In the Mboukoumassi potash deposit in the Republic of Congo (Congo-Brazzaville) in West Africa, several layers of organic-rich dark shale were found in the evaporite series. Through drilling core, the dark shale in the evaporite is found to satisfy the requirements of Re-Os isotope test. The result shows that the Re-Os isochron age of the dark shale in the study area ranges from 78.7 ± 1.1 to 96 ± 7 Ma, which is the first precise age of the Mboukoumassi potash deposit in the Republic of Congo (Congo-Brazzaville), West Africa. Therefore, the age of deposition of this set of evaporite may be Cenomanian-Turonian, which is younger than the age previously thought (around 113-125Ma, Aptian). The Re-Os isotopic dating technique used for the pioneering study on the precise dating of the Mboukoumassi potash deposit provides a new approach to the study of the sedimentary age of ancient evaporite deposits. The initial 187Os/188Os value decreasing from 2.02 ± 0.21 to 0.982 ± 0.03 for the core sample reflects the source rock chang along the core, and this is consistent with the geological evolution of the basin.

Rainfall Controls on Land Surface Phenology over "Never-green" and "Ever-green" Lands in Africa

NASA Astrophysics Data System (ADS)

Yan, D.; Zhang, X.; Yu, Y.; Guo, W.

2015-12-01

The characteristics of land surface phenology (LSP) in the "Never-green" Sahara desert and the "Ever-green" equatorial Congo Basin were rarely discussed due to the extremely low seasonal greenness variations across the Sahara desert and the prolonged cloud cover over the Congo Basin. Based on 30-minute observations acquired by the Spinning Enhanced Visible and Infrared Imager onboard the METEOSAT geostationary satellites, we generated a three-day angularly corrected Two-band Enhanced Vegetation Index (EVI2) time series for each year between 2006 and 2013. We further reconstructed EVI2 temporal trajectories and retrieved LSP transitions using the Hybrid Piecewise Logistic Model. We associated the LSP transitions with the rainy season transitions derived from the Tropical Rainfall Measurement Mission Product 3B42. Results show that LSP within both the Sahara Desert and the Congo Basin was strongly controlled by the rainfall seasonality. Specially, although there is no vegetation growth in most part of the Sahara Desert, recurring LSP was spatially detected in irrigation agriculture and the geomorphological regions of wadis, dayas, chotts/sebkhas and rocky hills. These geomorphological features are able to store moisture in soil to keep plants growing during the long dry seasons after vegetation greenup is triggered by rainfall events. The spatial shift of phenological timing is controlled by the Mediterranean rainfall regime in the north and the rainfalls brought by the Intertropical Convergence Zone (ITCZ) in the south. Across the equatorial Congo Basin, EVI2 time series reveals that canopy greenness cycles (CGC) of the seasonal leaf variation occur in tropical rainforests, which differs from the commonly termed "growing season" with complete leafless canopies. The seasonal EVI2 amplitude is very small and represents the gradual "leaf-exchange" processes. Two annual CGC are found and their spatial shifts closely follow the seasonal migration of ITCZ precipitation.

Description of Hylopanchax paucisquamatus (Cyprinodontiformes: Poeciliidae), a new lampeye species from the Odzala-Kokoua National Park, Republic of Congo.

PubMed

Sonnenberg, Rainer; Friel, John P; Van der Zee, Jouke R

2014-08-05

A new deep-bodied Hylopanchax species is described from the northwestern Congo basin. Hylopanchax paucisquamatus, new species, was collected in the Odzala-Kokoua National Park in the Likouala River drainage of the Republic of Congo. It differs from its congeners, including the deep-bodied H. leki and H. ndeko, by a unique combination of morphological characters, including low number of mid-longitudinal and transverse scales, number of dorsal-fin rays, and position of dorsal-fin origin in relation to anal-fin. It is the only deep-bodied species currently known outside the Kasaï River drainage.

Divergent biophysical controls of aquatic CO2 and CH4 in the World's two largest rivers.

PubMed

Borges, Alberto V; Abril, Gwenaël; Darchambeau, François; Teodoru, Cristian R; Deborde, Jonathan; Vidal, Luciana O; Lambert, Thibault; Bouillon, Steven

2015-10-23

Carbon emissions to the atmosphere from inland waters are globally significant and mainly occur at tropical latitudes. However, processes controlling the intensity of CO2 and CH4 emissions from tropical inland waters remain poorly understood. Here, we report a data-set of concurrent measurements of the partial pressure of CO2 (pCO2) and dissolved CH4 concentrations in the Amazon (n = 136) and the Congo (n = 280) Rivers. The pCO2 values in the Amazon mainstem were significantly higher than in the Congo, contrasting with CH4 concentrations that were higher in the Congo than in the Amazon. Large-scale patterns in pCO2 across different lowland tropical basins can be apprehended with a relatively simple statistical model related to the extent of wetlands within the basin, showing that, in addition to non-flooded vegetation, wetlands also contribute to CO2 in river channels. On the other hand, dynamics of dissolved CH4 in river channels are less straightforward to predict, and are related to the way hydrology modulates the connectivity between wetlands and river channels.

Comparative Proteomics of Human Monkeypox and Vaccinia Intracellular Mature and Extracellular Enveloped Virions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Manes, Nathan P.; Estep, Ryan D.; Mottaz, Heather M.

2008-03-07

Orthopoxviruses are the largest and most complex of the animal viruses. In response to the recent emergence of monkeypox in Africa and the threat of smallpox bioterrorism, virulent (monkeypox virus) and benign (vaccinia virus) orthopoxviruses were proteomically compared with the goal of identifying proteins required for pathogenesis. Orthopoxviruses were grown in HeLa cells to two different viral forms (intracellular mature virus and extracellular enveloped virus), purified by sucrose gradient ultracentrifugation, denatured using RapiGest™ surfactant, and digested with trypsin. Unfractionated samples and strong cation exchange HPLC fractions were analyzed by reversed-phase LC-MS/MS, and analyses of the MS/MS spectra using SEQUEST® andmore » X! Tandem resulted in the identification of hundreds of monkeypox, vaccinia, and copurified host proteins. The unfractionated samples were additionally analyzed by LC-MS on an LTQ-Orbitrap™, and the accurate mass and elution time tag approach was used to perform quantitative comparisons. Possible pathophysiological roles of differentially expressed orthopoxvirus genes are discussed.« less

DOE Office of Scientific and Technical Information (OSTI.GOV)

Burwood, R.; Mycke, B.

The Lower Congo Coastal and Kwanza provinces cumulatively account for reserves of ca 6 GBOR. These are dominantly reservoired in Pinda carbonate traps of the former basin. However, with production from a range of accretionary wedge, carbonate platform and Pre-Salt reservoirs, a diversity in oil character presupposes complex hydrocarbon habitats charged by multiple sourcing. Each of these two major Atlantic margin salt basins constitutes a different, source rock driven, hydrocarbon habitat. As classic passive margin pull-apart basins, Early Cretaceous initiated rift events (Pre-rift, Synrift I, II, etc.) evolved into the Drift phase opening of the southern Atlantic. A striking featuremore » of this progression was widespread evaporate deposition of the Aptian Loeme Salt. This separates two distinct sedimentary and tectonic domains of the Pre- and Post-Salt. The core Lower Congo habitat is dominated by the Pre-Salt Bucomazi (!) petroleum system. These lacustrine, often super-rich, sediments reveal considerable organofacies variations between their basin fill (Synrift I) and sheet drape (Synrift II) development, accounting for the compositional diversity in their progenic petroleums. Of crucial impact is a cognate diversity in their kerogen kinetic behaviour. This controls the conditions and timing of generation and realization of charge potential. With the Lower Congo habitat extending southwards to the Ambriz Spur, the Bucomazi facies proper appears restricted to the northern and deeper proto-lake trend. Over the more weakly subsident margins such troughs host inferior sheet drape potential. Elsewhere, the Upper Cretaceous-Paleogene marine clastic labe (!) petroleum system is hydrocarbon productive, yielding petroleums of unique, and/or mixed Pre-Salt, source provenance.« less

Therapeutic and prophylactic drugs to treat orthopoxvirus infections.

PubMed

Parker, Scott; Handley, Lauren; Buller, R Mark

2008-11-01

With the global eradication of smallpox in 1979, the causative agent, variola, no longer circulates in human populations. Other human poxvirus infections, such as those caused by vaccinia, cowpox virus and molluscum, are usually relatively benign in immunocompetent individuals. Conversely, monkeypox virus infections cause high levels of mortality and morbidity in Africa and the virus appears to be increasing its host range, virulence and demographic environs. Furthermore, there are concerns that clandestine stocks of variola virus exist. The re-introduction of aerosolized variola (or perhaps monkeypox virus) into human populations would result in high levels of morbidity and mortality. The attractiveness of variola as a bioweapon and, to a certain extent, monkeypox virus is its inherent ability to spread from person-to-person. The threat posed by the intentional release of variola or monkeypox virus, or a monkeypox virus epizoonosis, will require the capacity to rapidly diagnose the disease and to intervene with antivirals, as intervention is likely to take place during the initial diagnosis, approximately 10-15 days postinfection. Preimmunization of 'at-risk populations' with vaccines will likely not be practical, and the therapeutic use of vaccines has been shown to be ineffective after 4 days of infection with variola. However, a combination of vaccine and antivirals for those infected may be an option. Here we describe historical, current and future therapies to treat orthopoxvirus diseases.

Age, extent and carbon storage of the central Congo Basin peatland complex.

PubMed

Dargie, Greta C; Lewis, Simon L; Lawson, Ian T; Mitchard, Edward T A; Page, Susan E; Bocko, Yannick E; Ifo, Suspense A

2017-02-02

Peatlands are carbon-rich ecosystems that cover just three per cent of Earth's land surface, but store one-third of soil carbon. Peat soils are formed by the build-up of partially decomposed organic matter under waterlogged anoxic conditions. Most peat is found in cool climatic regions where unimpeded decomposition is slower, but deposits are also found under some tropical swamp forests. Here we present field measurements from one of the world's most extensive regions of swamp forest, the Cuvette Centrale depression in the central Congo Basin. We find extensive peat deposits beneath the swamp forest vegetation (peat defined as material with an organic matter content of at least 65 per cent to a depth of at least 0.3 metres). Radiocarbon dates indicate that peat began accumulating from about 10,600 years ago, coincident with the onset of more humid conditions in central Africa at the beginning of the Holocene. The peatlands occupy large interfluvial basins, and seem to be largely rain-fed and ombrotrophic-like (of low nutrient status) systems. Although the peat layer is relatively shallow (with a maximum depth of 5.9 metres and a median depth of 2.0 metres), by combining in situ and remotely sensed data, we estimate the area of peat to be approximately 145,500 square kilometres (95 per cent confidence interval of 131,900-156,400 square kilometres), making the Cuvette Centrale the most extensive peatland complex in the tropics. This area is more than five times the maximum possible area reported for the Congo Basin in a recent synthesis of pantropical peat extent. We estimate that the peatlands store approximately 30.6 petagrams (30.6 × 10 15  grams) of carbon belowground (95 per cent confidence interval of 6.3-46.8 petagrams of carbon)-a quantity that is similar to the above-ground carbon stocks of the tropical forests of the entire Congo Basin. Our result for the Cuvette Centrale increases the best estimate of global tropical peatland carbon stocks by 36 per cent, to 104.7 petagrams of carbon (minimum estimate of 69.6 petagrams of carbon; maximum estimate of 129.8 petagrams of carbon). This stored carbon is vulnerable to land-use change and any future reduction in precipitation.

Treatment with the smallpox antiviral tecovirimat (ST-246) alone or in combination with ACAM2000 vaccination is effective as a postsymptomatic therapy for monkeypox virus infection.

PubMed

Berhanu, Aklile; Prigge, Jonathan T; Silvera, Peter M; Honeychurch, Kady M; Hruby, Dennis E; Grosenbach, Douglas W

2015-07-01

The therapeutic efficacies of smallpox vaccine ACAM2000 and antiviral tecovirimat given alone or in combination starting on day 3 postinfection were compared in a cynomolgus macaque model of lethal monkeypox virus infection. Postexposure administration of ACAM2000 alone did not provide any protection against severe monkeypox disease or mortality. In contrast, postexposure treatment with tecovirimat alone or in combination with ACAM2000 provided full protection. Additionally, tecovirimat treatment delayed until day 4, 5, or 6 postinfection was 83% (days 4 and 5) or 50% (day 6) effective. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

In vitro efficacy of ST246 against smallpox and monkeypox.

PubMed

Smith, Scott K; Olson, Victoria A; Karem, Kevin L; Jordan, Robert; Hruby, Dennis E; Damon, Inger K

2009-03-01

Since the eradication of smallpox and the cessation of routine childhood vaccination for smallpox, the proportion of the world's population susceptible to infection with orthopoxviruses, such as variola virus (the causative agent of smallpox) and monkeypox virus, has grown substantially. In the United States, the only vaccines for smallpox licensed by the Food and Drug Administration (FDA) have been live virus vaccines. Unfortunately, a substantial number of people cannot receive live virus vaccines due to contraindications. Furthermore, no antiviral drugs have been fully approved by the FDA for the prevention or treatment of orthopoxvirus infection. Here, we show the inhibitory effect of one new antiviral compound, ST-246, on the in vitro growth properties of six variola virus strains and seven monkeypox virus strains. We performed multiple assays to monitor the cytopathic effect and to evaluate the reduction of viral progeny production and release in the presence of the compound. ST-246 had 50% effective concentrations of

Arsenic, Boron, and Fluoride Concentrations in Ground Water in and Near Diabase Intrusions, Newark Basin, Southeastern Pennsylvania

USGS Publications Warehouse

Senior, Lisa A.; Sloto, Ronald A.

2006-01-01

During an investigation in 2000 by the U.S. Environmental Protection Agency (USEPA) of possible contaminant releases from an industrial facility on Congo Road near Gilbertsville in Berks and Montgomery Counties, southeastern Pennsylvania, concentrations of arsenic and fluoride above USEPA drinking-water standards of 10 ?g/L and 4 mg/L, respectively, and of boron above the USEPA health advisory level of 600 ?g/L were measured in ground water in an area along the northwestern edge of the Newark Basin. In 2003, the USEPA requested technical assistance from the U.S. Geological Survey (USGS) to help identify sources of arsenic, boron, and fluoride in the ground water in the Congo Road area, which included possible anthropogenic releases and naturally occurring mineralization in the local bedrock aquifer, and to identify other areas in the Newark Basin of southeastern Pennsylvania with similarly elevated concentrations of these constituents. The USGS reviewed available data and collected additional ground-water samples in the Congo Road area and four similar hydrogeologic settings. The Newark Basin is the largest of the 13 major exposed Mesozoic rift basins that stretch from Nova Scotia to South Carolina. Rocks in the Newark Basin include Triassic through Jurassic-age sedimentary sequences of sandstones and shales that were intruded by diabase. Mineral deposits of hydrothermal origin are associated with alteration zones bordering intrusions of diabase and also occur as strata-bound replacement deposits of copper and zinc in sedimentary rocks. The USGS review of data available in 2003 showed that water from about 10 percent of wells throughout the Newark Basin of southeastern Pennsylvania had concentrations of arsenic greater than the USEPA maximum contaminant level (MCL) of 10 ?g/L; the highest reported arsenic concentration was at about 70 ?g/L. Few data on boron were available, and the highest reported boron concentration in well-water samples was 60 ?g/L in contrast to concentrations over 5,000 ?g/L in the Congo Road area. Although concentrations of fluoride up to 4 mg/L were reported for a few well-water samples collected throughout the Newark Basin, about 90 percent of the samples had concentrations of 0.5 mg/L or less. The USGS sampled 58 wells primarily in 5 areas in the Newark Basin, southeastern Pennsylvania, from February 2004 through April 2005 to identify other possible areas of elevated arsenic, boron, and fluoride and to characterize the geochemical environment associated with elevated concentrations of these constituents. Sampled wells included 12 monitor wells at an industrial facility near Congo Road, 45 private-supply wells in Berks, Montgomery, and Bucks Counties, and 1 private-supply well near Dillsburg, York County, an area where elevated fluoride in ground water had been reported in the adjacent Gettysburg Basin. Wells were sampled in transects from the diabase through the adjacent hornfels and into the unaltered shales of the Brunswick Group. Field measurements were made of pH, temperature, dissolved oxygen concentration, and specific conductance. Samples were analyzed in the laboratory for major ions, nutrients, total organic carbon, dissolved and total concentrations of selected trace elements, and boron isotopic composition. Generally, the ground water from the 46 private-supply wells had relatively neutral to alkaline pH (ranging from 6.1 to 9.1) and moderate concentrations of dissolved oxygen. Most water samples were of the calcium-bicarbonate type. Concentrations of arsenic up to 60 ?g/L, boron up to 3,950 ?g/L, and fluoride up to 0.70 mg/L were measured. Drinking-water standards or health advisories (for constituents that do not have standards established) were exceeded most frequently (about 20 percent of samples) for arsenic and boron and less frequently (6 percent or less of samples) for total iron, manganese, sulfate, nitrate, lead, molybdenum, and strontium. In water from 12 monitor

Divergent biophysical controls of aquatic CO2 and CH4 in the World’s two largest rivers

PubMed Central

Borges, Alberto V.; Abril, Gwenaël; Darchambeau, François; Teodoru, Cristian R.; Deborde, Jonathan; Vidal, Luciana O.; Lambert, Thibault; Bouillon, Steven

2015-01-01

Carbon emissions to the atmosphere from inland waters are globally significant and mainly occur at tropical latitudes. However, processes controlling the intensity of CO2 and CH4 emissions from tropical inland waters remain poorly understood. Here, we report a data-set of concurrent measurements of the partial pressure of CO2 (pCO2) and dissolved CH4 concentrations in the Amazon (n = 136) and the Congo (n = 280) Rivers. The pCO2 values in the Amazon mainstem were significantly higher than in the Congo, contrasting with CH4 concentrations that were higher in the Congo than in the Amazon. Large-scale patterns in pCO2 across different lowland tropical basins can be apprehended with a relatively simple statistical model related to the extent of wetlands within the basin, showing that, in addition to non-flooded vegetation, wetlands also contribute to CO2 in river channels. On the other hand, dynamics of dissolved CH4 in river channels are less straightforward to predict, and are related to the way hydrology modulates the connectivity between wetlands and river channels. PMID:26494107

Shift in the chemical composition of dissolved organic matter in the Congo River network

NASA Astrophysics Data System (ADS)

Lambert, Thibault; Bouillon, Steven; Darchambeau, François; Massicotte, Philippe; Borges, Alberto V.

2016-09-01

The processing of terrestrially derived dissolved organic matter (DOM) during downstream transport in fluvial networks is poorly understood. Here, we report a dataset of dissolved organic carbon (DOC) concentrations and DOM composition (stable carbon isotope ratios, absorption and fluorescence properties) acquired along a 1700 km transect in the middle reach of the Congo River basin. Samples were collected in the mainstem and its tributaries during high-water (HW) and falling-water (FW) periods. DOC concentrations and DOM composition along the mainstem were found to differ between the two periods because of a reduced lateral mixing between the central water masses of the Congo River and DOM-rich waters from tributaries and also likely because of a greater photodegradation during FW as water residence time (WRT) increased. Although the Cuvette Centrale wetland (one of the world's largest flooded forests) continuously releases highly aromatic DOM in streams and rivers of the Congo Basin, the downstream transport of DOM was found to result in an along-stream gradient from aromatic to aliphatic compounds. The characterization of DOM through parallel factor analysis (PARAFAC) suggests that this transition results from (1) the losses of aromatic compounds by photodegradation and (2) the production of aliphatic compounds by biological reworking of terrestrial DOM. Finally, this study highlights the critical importance of the river-floodplain connectivity in tropical rivers in controlling DOM biogeochemistry at a large spatial scale and suggests that the degree of DOM processing during downstream transport is a function of landscape characteristics and WRT.

Lightning climatology in the Congo Basin: methodology and first results

NASA Astrophysics Data System (ADS)

Kigotsi, Jean; Soula, Serge; Georgis, Jean-François; Barthe, Christelle

2016-04-01

The global climatology of lightning issued from space observations (OTD and LIS) clearly showed the maximum of the thunderstorm activity is located in a large area of the Congo Basin, especially in the Democratic Republic of Congo (DRC). The first goal of the present study is to compare observations from the World Wide Lightning Location Network (WWLLN) from the Lightning Imaging Sensor (LIS) over a 9-year period (2005-2013) in this 2750 km × 2750 km area. The second goal is to analyse the lightning activity in terms of time and space variability. The detection efficiency (DE) of the WWLLN relative to LIS has increased between 2005 and 2013, typically from about 1.70 % to 5.90 %, in agreement with previous results for other regions of the world. The mean monthly flash rate describes an annual cycle with a maximum between November and March and a minimum between June and August, associated with the ICTZ migration but not exactly symmetrical on both sides of the equator. The diurnal evolution of the flash rate has a maximum between 1400 and 1700 UTC, depending on the reference year, in agreement with previous works in other regions of the world. The annual flash density shows a sharp maximum localized in eastern DRC regardless of the reference year and the period of the year. This annual maximum systematically located west of Kivu Lake corresponds to that previously identified by many authors as the worldwide maximum which Christian et al. (2013) falsely attributed to Rwanda. Another more extended region within the Congo Basin exhibits moderately large values, especially during the beginning of the period analyzed. A comparison of both patterns of lightning density from the WWLLN and from LIS allows to validate the representativeness of this world network and to restitute the total lightning activity in terms of lightning density and rate.

A Nosocomial Outbreak of Human Monkeypox in the Central African Republic

PubMed Central

Nakoune, Emmanuel; Lampaert, Emmanuel; Ndjapou, Séverin Gervais; Janssens, Carole; Zuniga, Isabel; Van Herp, Michel; Fongbia, Jean Paul; Koyazegbe, Thomas Daquin; Selekon, Benjamin; Komoyo, Giscard Francis; Garba-Ouangole, Sandra Miriella; Manengu, Casimir; Manuguerra, Jean-Claude; Kazanji, Mirdad; Gessain, Antoine; Berthet, Nicolas

2017-01-01

Abstract An outbreak of familial monkeypox occurred in the Central African Republic in 2015/2016 by 3 transmission modes: familial, health care–related, and transport-related. Ten people (3 children and 7 adults) were infected. Most presented with cutaneous lesions and fever, and 2 children died. The viral strain responsible was a Zaire genotype strain. PMID:29732376

Dynamical and Thermodynamic Elements of Modeled Climate Change at the East African Margin of Convection

NASA Astrophysics Data System (ADS)

Giannini, Alessandra; Lyon, Bradfield; Seager, Richard; Vigaud, Nicolas

2018-01-01

We propose a dynamical interpretation of model projections for an end-of-century wetting in equatorial East Africa. In the current generation of global climate models, increased atmospheric moisture content associated with warming is not the dominant process explaining the increase in rainfall, as the regional circulation is only weakly convergent even during the rainy seasons. Instead, projected wetter future conditions are generally consistent with the El Niño-like trend in tropical Pacific sea surface temperatures in climate models. In addition, a weakening in moisture convergence over the adjacent Congo Basin and Maritime Continent cores of convection results in the weakening of near-surface winds, which increases moisture advection from the Congo Basin core toward the East African margin. Overall confidence in the projections is limited by the significant biases in simulation of the regional climatology and disagreement between observed and modeled tropical Pacific sea surface temperature trends to date.

Differences between Pygmy and Non-Pygmy Hunting in Congo Basin Forests.

PubMed

Fa, John E; Olivero, Jesús; Farfán, Miguel Angel; Lewis, Jerome; Yasuoka, Hirokazu; Noss, Andrew; Hattori, Shiho; Hirai, Masaaki; Kamgaing, Towa O W; Carpaneto, Giuseppe; Germi, Francesco; Márquez, Ana Luz; Duarte, Jesús; Duda, Romain; Gallois, Sandrine; Riddell, Michael; Nasi, Robert

2016-01-01

We use data on game harvest from 60 Pygmy and non-Pygmy settlements in the Congo Basin forests to examine whether hunting patterns and prey profiles differ between the two hunter groups. For each group, we calculate hunted animal numbers and biomass available per inhabitant, P, per year (harvest rates) and killed per hunter, H, per year (extraction rates). We assess the impact of hunting of both hunter groups from estimates of numbers and biomass of prey species killed per square kilometre, and by examining the proportion of hunted taxa of low, medium and high population growth rates as a measure of their vulnerability to overhunting. We then map harvested biomass (kg-1P-1Yr-1) of bushmeat by Pygmies and non-Pygmies throughout the Congo Basin. Hunting patterns differ between Pygmies and non-Pygmies; Pygmies take larger and different prey and non-Pygmies sell more for profit. We show that non-Pygmies have a potentially more severe impact on prey populations than Pygmies. This is because non-Pygmies hunt a wider range of species, and twice as many animals are taken per square kilometre. Moreover, in non-Pygmy settlements there was a larger proportion of game taken of low population growth rate. Our harvest map shows that the non-Pygmy population may be responsible for 27 times more animals harvested than the Pygmy population. Such differences indicate that the intense competition that may arise from the more widespread commercial hunting by non-Pygmies is a far more important constraint and source of conflict than are protected areas.

Differences between Pygmy and Non-Pygmy Hunting in Congo Basin Forests

PubMed Central

Olivero, Jesús; Farfán, Miguel Angel; Lewis, Jerome; Yasuoka, Hirokazu; Noss, Andrew; Hattori, Shiho; Hirai, Masaaki; Kamgaing, Towa O. W.; Carpaneto, Giuseppe; Germi, Francesco; Márquez, Ana Luz; Duarte, Jesús; Duda, Romain; Gallois, Sandrine; Riddell, Michael; Nasi, Robert

2016-01-01

We use data on game harvest from 60 Pygmy and non-Pygmy settlements in the Congo Basin forests to examine whether hunting patterns and prey profiles differ between the two hunter groups. For each group, we calculate hunted animal numbers and biomass available per inhabitant, P, per year (harvest rates) and killed per hunter, H, per year (extraction rates). We assess the impact of hunting of both hunter groups from estimates of numbers and biomass of prey species killed per square kilometre, and by examining the proportion of hunted taxa of low, medium and high population growth rates as a measure of their vulnerability to overhunting. We then map harvested biomass (kg-1P-1Yr-1) of bushmeat by Pygmies and non-Pygmies throughout the Congo Basin. Hunting patterns differ between Pygmies and non-Pygmies; Pygmies take larger and different prey and non-Pygmies sell more for profit. We show that non-Pygmies have a potentially more severe impact on prey populations than Pygmies. This is because non-Pygmies hunt a wider range of species, and twice as many animals are taken per square kilometre. Moreover, in non-Pygmy settlements there was a larger proportion of game taken of low population growth rate. Our harvest map shows that the non-Pygmy population may be responsible for 27 times more animals harvested than the Pygmy population. Such differences indicate that the intense competition that may arise from the more widespread commercial hunting by non-Pygmies is a far more important constraint and source of conflict than are protected areas. PMID:27589384

Exploring evapotranspiration dynamics over Sub-Sahara Africa (2000-2014).

PubMed

Ndehedehe, Christopher E; Okwuashi, Onuwa; Ferreira, Vagner G; Agutu, Nathan O

2018-06-14

Monitoring changes in evapotranspiration (ET) is useful in the management of water resources in irrigated agricultural landscapes and in the assessment of crop stress and vegetation conditions of drought-vulnerable regions. Information on the impacts of climate variability on ET dynamics is profitable in developing water management adaptation strategies. Such impacts, however, are generally unreported and not conclusively determined in some regions. In this study, changes in MODIS (Moderate Resolution Imaging Spectroradiometer)-derived ET (2000-2014) over large proportions of Sub-Sahara Africa (SSA) are explored. The multivariate analyses of ET over SSA showed that four leading modes of observed dynamics in ET, accounting for about 90% of the total variability, emanated mostly from some sections of the Sudano-Sahel and Congo basin. Based on Man-Kendall's statistics, significant positive trends (α = 0.05) in ET over the Central African Republic and most parts of the Sahel region were observed. Over much of the Congo basin nonetheless, ET showed significant (α = 0.05) distributions of widespread negative trends. These trends in ET were rather found to be consistent with observed changes in model soil moisture but not in all locations, perhaps due to inconsistent trends in maximum rainfall and land surface temperature. However, the results of spatio-temporal drought analysis confirm that the extensive ET losses in the Congo basin were somewhat induced by soil moisture deficits. Amidst other prominent drivers of ET, the dynamics of ET over the terrestrial ecosystems of SSA appear to be a more complex phenomenon that may transcend natural climate variations.

The biogeochemistry of carbon across a gradient of streams and rivers within the Congo Basin

NASA Astrophysics Data System (ADS)

Mann, P. J.; Spencer, R. G. M.; Dinga, B. J.; Poulsen, J. R.; Hernes, P. J.; Fiske, G.; Salter, M. E.; Wang, Z. A.; Hoering, K. A.; Six, J.; Holmes, R. M.

2014-04-01

Dissolved organic carbon (DOC) and inorganic carbon (DIC, pCO2), lignin biomarkers, and theoptical properties of dissolved organic matter (DOM) were measured in a gradient of streams and rivers within the Congo Basin, with the aim of examining how vegetation cover and hydrology influences the composition and concentration of fluvial carbon (C). Three sampling campaigns (February 2010, November 2010, and August 2011) spanning 56 sites are compared by subbasin watershed land cover type (savannah, tropical forest, and swamp) and hydrologic regime (high, intermediate, and low). Land cover properties predominately controlled the amount and quality of DOC, chromophoric DOM (CDOM) and lignin phenol concentrations (∑8) exported in streams and rivers throughout the Congo Basin. Higher DIC concentrations and changing DOM composition (lower molecular weight, less aromatic C) during periods of low hydrologic flow indicated shifting rapid overland supply pathways in wet conditions to deeper groundwater inputs during drier periods. Lower DOC concentrations in forest and swamp subbasins were apparent with increasing catchment area, indicating enhanced DOC loss with extended water residence time. Surface water pCO2 in savannah and tropical forest catchments ranged between 2,600 and 11,922 µatm, with swamp regions exhibiting extremely high pCO2 (10,598-15,802 µatm), highlighting their potential as significant pathways for water-air efflux. Our data suggest that the quantity and quality of DOM exported to streams and rivers are largely driven by terrestrial ecosystem structure and that anthropogenic land use or climate change may impact fluvial C composition and reactivity, with ramifications for regional C budgets and future climate scenarios.

National forest cover change in Congo Basin: deforestation, reforestation, degradation and regeneration for the years 1990, 2000 and 2005.

PubMed

Céline, Ernst; Philippe, Mayaux; Astrid, Verhegghen; Catherine, Bodart; Musampa, Christophe; Pierre, Defourny

2013-04-01

This research refers to an object-based automatic method combined with a national expert validation to produce regional and national forest cover change statistics over Congo Basin. A total of 547 sampling sites systematically distributed over the whole humid forest domain are required to cover the six Central African countries containing tropical moist forest. High resolution imagery is used to accurately estimate not only deforestation and reforestation but also degradation and regeneration. The overall method consists of four steps: (i) image automatic preprocessing and preinterpretation, (ii) interpretation by national expert, (iii) statistic computation and (iv) accuracy assessment. The annual rate of net deforestation in Congo Basin is estimated to 0.09% between 1990 and 2000 and of net degradation to 0.05%. Between 2000 and 2005, this unique exercise estimates annual net deforestation to 0.17% and annual net degradation to 0.09%. An accuracy assessment reveals that 92.7% of tree cover (TC) classes agree with independent expert interpretation. In the discussion, we underline the direct causes and the drivers of deforestation. Population density, small-scale agriculture, fuelwood collection and forest's accessibility are closely linked to deforestation, whereas timber extraction has no major impact on the reduction in the canopy cover. The analysis also shows the efficiency of protected areas to reduce deforestation. These results are expected to contribute to the discussion on the reduction in CO2 emissions from deforestation and forest degradation (REDD+) and serve as reference for the period. © 2012 Blackwell Publishing Ltd.

Contrasting biogeochemical characteristics of the Oubangui River and tributaries (Congo River basin)

PubMed Central

Bouillon, Steven; Yambélé, Athanase; Gillikin, David P.; Teodoru, Cristian; Darchambeau, François; Lambert, Thibault; Borges, Alberto V.

2014-01-01

The Oubangui is a major tributary of the Congo River. We describe the biogeochemistry of contrasting tributaries within its central catchment, with watershed vegetation ranging from wooded savannahs to humid rainforest. Compared to a 2-year monitoring record on the mainstem Oubangui, these tributaries show a wide range of biogeochemical signatures, from highly diluted blackwaters (low turbidity, pH, conductivity, and total alkalinity) in rainforests to those more typical for savannah systems. Spectral analyses of chromophoric dissolved organic matter showed wide temporal variations in the Oubangui compared to spatio-temporal variations in the tributaries, and confirm that different pools of dissolved organic carbon are mobilized during different hydrological stages. δ13C of dissolved inorganic carbon ranged between −28.1‰ and −5.8‰, and was strongly correlated to both partial pressure of CO2 and to the estimated contribution of carbonate weathering to total alkalinity, suggesting an important control of the weathering regime on CO2 fluxes. All tributaries were oversaturated in dissolved greenhouse gases (CH4, N2O, CO2), with highest levels in rivers draining rainforest. The high diversity observed underscores the importance of sampling that covers the variability in subcatchment characteristics, to improve our understanding of biogeochemical cycling in the Congo Basin. PMID:24954525

What Controls the Hydrodynamics of the Central Congo River?

NASA Astrophysics Data System (ADS)

O'Loughlin, F.; Bates, P. D.

2014-12-01

Despite being the second largest river basin in the world, with a drainage area greater than 3.7 million square kilometres, little is known about the hydraulics of the Congo River. This lack of knowledge is mainly due to a mixture of conflicts and the difficulty of accessing existing data. We present results of studies which have focused primarily on the middle reach of the Congo River, located between Kisangani and Kinshasa, and its six main tributaries (Kasai, Ubangai, Sangha, Ruki, Lulonga and Lomami rivers). Through a combination of remotely sensed datasets and a hydrodynamic model we investigated what factors control the hydrodynamics of the middle reach. From the analysis of the remotely sensed datasets, we discover that variability in river width of the middle reach of the Congo is large and cannot be represented by empirical equations which relate channel geometry to basin area and discharge. Water surface slopes vary from 3.5 cm/km to 9 cm/km, which is far more than previous studies suggest. The remote datasets indicate that there exist 5 large constrictions in the river width which may result in backwater affecting between 11 and 33 percent of middle reach at low and high water respectively. These results were corroborated by the hydrodynamic model. In fact, when all constrictions caused by a narrowing in width of 1 km or more are considered, water levels along 43 percent of the middle reach change by at least 0.5 m. Using the hydrodynamic model we also investigated the importance of the wetlands to the attenuation of the flood wave through the system. Initial results suggest that for the Congo River, floodplains have far more impact on the peak magnitude than the timing of the flood wave. When the model was run with no floodplain interactions an increase in the magnitude of flood peak was observed, with the timing of the waves being consistent with observed measurements.

Evolution and estimated age of the C5 Lukala carbonate-evaporite ramp complex in the Lower Congo region (Democratic Republic of Congo): New perspectives in Central Africa

NASA Astrophysics Data System (ADS)

Delpomdor, F.; Van Vliet, N.; Devleeschouwer, X.; Tack, L.; Préat, A.

2018-01-01

New detailed lithological, sedimentological, chemostratigraphic data were obtained from exploration drilling samples on the C5 carbonate-dominated formation of the Neoproterozoic Lukala Subgroup (former Schisto-Calcaire Subgroup) from the West Congo Belt (WCB) in the Democratic Republic of Congo. This formation records the last post-Marinoan sea-level events that occurred in the whole basin, followed by the development of the Araçuaï-West Congo Orogen between 630 and 560 Ma. The C5 Formation consists of back-reef lagoonal and peritidal/sabkha cycles of ∼2.0 m in thickness, that record a short-time marine regression, rapidly flooded by a marine transgression with deposition of organic-rich argillaceous carbonates or shales under dysoxia and anoxia conditions. These dysoxic/anoxic waters were rapidly followed by a regional-scale marine transgression, favouring mixing with well-oxygenated waters, and the development of benthic Tonian to Cambro-Ordovician Obruchevella parva-type 'seagrasses' in the nearshore zones of the lagoons. New δ13C and 87Sr/86Sr isotopic data in the C5 Formation of the Lukala Subgroup are used in the frame of a correlation with the Sete Lagoas Formation in Brazil. Relatively comparable negative to positive δ13C excursions point to marine flooding of the whole basin and allow extension of the debatable Late Ediacaran age of the uppermost Sete Lagoas and C5 formations. Sr isotope ;blind dating; failed due to low Sr concentration related to a dolomitization event close 540 Ma. Several tentative datings of the C5 Formation converge to a Late Ediacaran age ranging between 575 and 540 Ma. As the overlying Mpioka folded Subgroup, the C5 series suffered the Pan African deformation, dated at 566 ± 42 Ma. Unlike the previously generally accepted interpretation, our data suggests that the Mpioka Subgroup was deposited in the Early Cambrian.

Dissolved major elements exported by the Congo and the Ubangi rivers during the period 1987 1989

NASA Astrophysics Data System (ADS)

Probst, Jean-Luc; NKounkou, Renard-Roger; Krempp, Gérard; Bricquet, Jean-Pierre; Thiébaux, Jean-Pierre; Olivry, Jean-Claude

1992-07-01

On the basis of monthly sampling during the period 1987-1989, the geochemistry of the Congo and the Ubangi (second largest tributary of the Congo) rivers was studied in order (1) to understand the seasonal variations of the physico-chemical parameters of the waters and (2) to estimate the annual dissolved fluxes exported by the two rivers. The results presented here correspond to the first three years of measurements carried out for a scientific programme (Interdisciplinary Research Programme on Geodynamics of Peri-Atlantic Intertropical Environments, Operation 'Large River Basins' (PIRAT-GBF) undertaken jointly by Institut National des Sciences de l'Univers (INSU) and Institut Français de Recherche Scientifique pour le Développement en Coopération (ORSTOM) planned to run for at least ten years. The Congo River is more diluted than the Ubangi (34 mgl -1 vs. 42 mgl -1). For both rivers, the inorganic dissolved load is composed mainly of HCO 3- and SiO 2. The chemical composition of the water does not change with time. In the Ubangi River, because of the presence of Precambrian carbonate rocks in its catchment, the proportions of HCO 3- and Ca 2+ are higher. On a seasonal scale, the concentration of dissolved cations and anions varies inversely with discharge, except silica. The comparison of the discharge-concentration relationship with a theoretical 'zero dilution' shows that the evolution of the concentration of dissolved substances is a simple dilution by the surface waters, with, in the case of the Ubangi, a small supply of dissolved substances by the surface waters. Using three different methods of calculation, the estimated annual inorganic dissolved flux of the Congo ranges from 39 × 10 6 to 44 × 10 6 tons (according to the year), with about 10% of this coming from the Ubangi drainage basin.

Anticipating the Species Jump: Surveillance for Emerging Viral Threats

DTIC Science & Technology

2010-12-01

views expressed herein are those of the author and do not necessarily reflect the official policy or position of the Defense Threat Reduction Agency...Germany** [10, 11] Hantavirus (Sin Nombre virus) Deer mouse (Peromyscus maniculatus) 1993 Four Corners area, US [12] Monkeypox (Monkeypox virus...genetically diverse Marburg viruses from Egyptian fruit bats." PLoS Pathog 5(7): e1000536. 12. "Update: Outbreak of Hantavirus Infection — Southwestern

Simple Technique for in Field Samples Collection in the Cases of Skin Rash Illness and Subsequent PCR Detection of Orthopoxviruses and Varicella Zoster Virus

PubMed Central

Magazani, Edmond K.; Garin, Daniel; Muyembe, Jean-Jacques T.; Bentahir, Mostafa; Gala, Jean-Luc

2014-01-01

Background In case of outbreak of rash illness in remote areas, clinically discriminating monkeypox (MPX) from severe form of chickenpox and from smallpox remains a concern for first responders. Objective The goal of the study was therefore to use MPX and chickenpox outbreaks in Democratic Republic of Congo (DRC) as a test case for establishing a rapid and specific diagnosis in affected remote areas. Methods In 2008 and 2009, successive outbreaks of presumed MPX skin rash were reported in Bena Tshiadi, Yangala and Ndesha healthcare districts of the West Kasai province (DRC). Specimens consisting of liquid vesicle dried on filter papers or crusted scabs from healing patients were sampled by first responders. A field analytical facility was deployed nearby in order to carry out a real-time PCR (qPCR) assay using genus consensus primers, consensus orthopoxvirus (OPV) and smallpox-specific probes spanning over the 14 kD fusion protein encoding gene. A PCR-restriction fragment length polymorphism was used on-site as backup method to confirm the presence of monkeypox virus (MPXV) in samples. To complete the differential diagnosis of skin rash, chickenpox was tested in parallel using a commercial qPCR assay. In a post-deployment step, a MPXV-specific pyrosequencing was carried out on all biotinylated amplicons generated on-site in order to confirm the on-site results. Results Whereas MPXV proved to be the agent causing the rash illness outbreak in the Bena Tshiadi, VZV was the causative agent of the disease in Yangala and Ndesha districts. In addition, each on-site result was later confirmed by MPXV-specific pyrosequencing analysis without any discrepancy. Conclusion This experience of rapid on-site dual use DNA-based differential diagnosis of rash illnesses demonstrates the potential of combining tests specifically identifying bioterrorism agents and agents causing natural outbreaks. This opens the way to rapid on-site DNA-based identification of a broad spectrum of causative agents in remote areas. PMID:24841633

Dynamic behaviour of natural oil droplets through the water column in deep-water environment: the case of the Lower Congo Basin

NASA Astrophysics Data System (ADS)

Jatiault, R.; Dhont, D.; Loncke, L.; Durrieu De Madron, X.; Dubucq, D.; Channelliere, C.; Bourrin, F.

2017-12-01

Key words: Hydrocarbon seepage, Oil Slick, Lower Congo Basin, Underwater deflection, Deep-water Pockmark, Ascent speedThe space-borne imagery provides a significant means to locate active oil seeps and to estimate the expelled volume in the marine environment. The analysis of numerous overlapping satellite images revealed an abundant volume of 4400 m3 of oil naturally reaching the sea surface per year, expelled from more than a hundred seep sites through the Lower Congo Basin. The active seepage area is located in the distal compressional province of the basin where salt napes and squeezed diapirs. The integration of current data was used to link accurately sea surface manifestations of natural oil leakages with active fluid flow features on the seafloor. A mooring with ADCPs (Acoustic Doppler Current Profilers) distributed throughout the water column provided an efficient calibration tool to evaluate the horizontal deflection of oil droplets. Using a Eulerian propagation model that considered a range of probable ascent speeds, we estimated the oil migration pathways through the water column using two different approaches. The first approach consisted in simulating the backwards trajectory of oil droplets using sea surface oil slicks locations and concomitant current measurements. The second method analyzed the spatial spreading of the surfacing signatures of natural oil slicks based on 21 years of satellite observations. The location of the surfacing points of oil droplets at the sea surface is restricted to a circle of 2.5 km radius around the release point at the seafloor. Both approaches provided a range of ascent speeds of oil droplets between 3 to 8 cm.s-1. The low deflection values validate the near-vertical links between the average surfacing area of oil slicks at the sea surface with specific seafloor disturbances (i.e. pockmarks or mounds) known to expel fluids.

A Study of Waste Management within the COL Florence A. Blanchfield Army Community Hospital, Fort Campbell, Kentucky.

DTIC Science & Technology

1981-08-01

besnoiti Borna disease virus Bovine infectious petechial fever virus Camel pox virus Ephemeral fever virus Fowl plague virus Goat pox virus Hog...Varicella virus Vole rickettsia Yellow fever virus, 17D vaccine strain 69 Class 3 Alastrun, smallpox, monkeypox, and whitepox, when used in vitro Arbovirus...animal inoculation experiments Vesicular stomatitis virus Yellow fever virus - wild when used in vitro Class 4 Alastrun, smallpox, monkeypox, and

[Monkeypox: second human case observed in Ivory Coast (rural health sector of Daloa].

PubMed

Merouze, F; Lesoin, J J

1983-01-01

A second case of human monkeypox (world fifty-forth case) has been observed in Ivory Coast (rural health sector of Daloa). A three years old girl presented a major pox-like eruption which evolved to recovery. The authors describe the eruption, the evolution of the sickness, and the scars observed at the fourth month after recovery. It has not been possible to prove neither animal-human nor interhuman contagion.

Smallpox DNA Vaccine Protects Nonhuman Primates Against Lethal Monkeypox

DTIC Science & Technology

2004-05-01

skin, the vaccine itself can pose a serious health risk. Here, we demonstrate that rhesus macaques vaccinated with a DNA vaccine consisting of four...administered to the skin, the vaccine itself can pose a serious health risk. Here, we demonstrate that rhesus macaques vaccinated with a DNA vaccine consisting...vaccine to protect rhesus macaques from severe monkeypox. MATERIALS AND METHODS Viruses and cells. The VACV Connaught vaccine strain (derived from the New

Gene Expression Profiling of Monkeypox Virus-Infected Cells Reveals Novel Interfaces for Host-Virus Interactions

DTIC Science & Technology

2010-07-28

expression is plotted on Y -axis after normalization to mock-treated samples. Results plotted to compare calculated fold change in expression of each gene ...RESEARCH Open Access Gene expression profiling of monkeypox virus-infected cells reveals novel interfaces for host-virus interactions Abdulnaser...suppress antiviral cell defenses, exploit host cell machinery, and delay infection-induced cell death. However, a comprehensive study of all host genes

Changes in hydro-meteorological conditions over tropical West Africa (1980-2015) and links to global climate

NASA Astrophysics Data System (ADS)

Ndehedehe, Christopher E.; Awange, Joseph L.; Agutu, Nathan O.; Okwuashi, Onuwa

2018-03-01

The role of global sea surface temperature (SST) anomalies in modulating rainfall in the African region has been widely studied and is now less debated. However, their impacts and links to terrestrial water storage (TWS) in general, have not been studied. This study presents the pioneer results of canonical correlation analysis (CCA) of TWS derived from both global reanalysis data (1980-2015) and GRACE (Gravity Recovery and Climate Experiment) (2002-2014) with SST fields. The main issues discussed include, (i) oceanic hot spots that impact on TWS over tropical West Africa (TWA) based on CCA, (ii) long term changes in model and global reanalysis data (soil moisture, TWS, and groundwater) and the influence of climate variability on these hydrological indicators, and (iii) the hydrological characteristics of the Equatorial region of Africa (i.e., the Congo basin) based on GRACE-derived TWS, river discharge, and precipitation. Results of the CCA diagnostics show that El-Niño Southern Oscillation related equatorial Pacific SST fluctuations is a major index of climate variability identified in the main portion of the CCA procedure that indicates a significant association with long term TWS reanalysis data over TWA (r = 0.50, ρ < 0.05). Based on Mann-Kendall's statistics, the study found fairly large long term declines (ρ < 0.05) in TWS and soil moisture (1982 - 2015), mostly over the Congo basin, which coincided with warming of the land surface and the surrounding oceans. Meanwhile, some parts of the Sahel show significant wetting (rainfall, soil moisture, groundwater, and TWS) trends during the same period (1982-2015) and aligns with the ongoing narratives of rainfall recovery in the region. Results of singular spectral analysis and regression confirm that multi-annual changes in the Congo River discharge explained a considerable proportion of variability in GRACE-hydrological signal over the Congo basin (r = 0.86 and R2 = 0.70, ρ < 0.05). Finally, leading orthogonal modes of MERRA and GRACE-TWS over TWA show significant association with global SST anomalies.

Contrasting biogeochemical characteristics of right-bank tributaries of the Oubangui River, and a comparison with the mainstem river (Congo basin, Central African Republic).

NASA Astrophysics Data System (ADS)

Bouillon, Steven; Yambélé, Athanase; Gillikin, David P.; Teodoru, Cristian; Darchambeau, François; Lambert, Thibault; Borges, Alberto V.

2014-05-01

The Oubangui is a major right-bank tributary of the Congo River, draining an area of ~500,000 km² of mainly wooded savannahs. Here, we describe data on the physico-chemical characteristics and biogeochemistry of contrasting tributaries within the central Oubangui catchment collected during 3 field surveys between 2010 and 2012, with land use ranging from wooded savannahs to humid tropical rainforest. Compared to data from two years of sampling at high temporal resolution on the mainstem river in Bangui (Central African Republic), these tributaries show a remarkably wide range of biogeochemical signatures, from highly diluted blackwaters (low turbidity, pH, conductivity and total alkalinity (TA)) in rivers draining dense rainforests to those more typical for (sub)tropical savannah systems. Based on carbon stable isotope data (δ13C), the majority of sites show a corresponding dominance of C3-derived organic matter, with a tendency for increased C4 contributions the more turbid sites such as the Mpoko River. δ13C of dissolved organic carbon (DOC) were generally similar to those of particulate organic carbon (POC) across the different tributaries. δ13C of dissolved inorganic carbon (DIC) ranged between -28.1 ‰ in low-TA rainforest (blackwater) rivers to -5.8 ‰ in the mainstem Oubangui. These variations were strongly correlated to both partial pressure of CO2 (pCO2) and to the estimated contribution of carbonate weathering to total alkalinity, suggesting an important control of the dominant weathering regime (silicate versus carbonate weathering) on DIC and CO2 fluxes. All tributaries were consistently oversaturated in dissolved greenhouse gases (CH4, N2O, and CO2) with respect to atmospheric equilibrium, with highest levels observed in rivers draining rainforest vegetation. The high diversity observed within this subcatchment of the Congo River basin is equivalent to that observed in much larger, heterogeneous catchments, and underscores the importance of sampling at the wider scale, covering the variability in subcatchment characteristics, to improve our understanding of biogeochemical cycling in the Congo Basin.

Paleoenvironments, δ13C and δ18O signatures in the Neoproterozoic carbonates of the Comba Basin, Republic of Congo: Implications for regional correlations and Marinoan event

NASA Astrophysics Data System (ADS)

Préat, Alain; Delpomdor, Franck; Ackouala Mfere, Anna Perla; Callec, Yannick

2018-01-01

The Ediacaran Schisto-Calcaire Group is a ∼1300 m-thick succession belonging to the West Congo Supergroup in Central Africa. In the Comba Basin, it consists of three carbonate-dominated units defined as formations (SCI to SCIII) that are unconformably overlain by clastic deposits (Mpioka Group) interpreted as a molassic formation associated with the Panafrican Orogen. The underlying Upper Tillite and Cap Carbonate (SCIa) units, considered as markers of the Snowball Earth event were studied in three sections. We investigated the carbonates of the Schisto-Calcaire Group by defining new microfacies (MF1-MF7) and we performed C and O isotopic analyses in order to constraint the depositional and diagenetic events directly after the Marinoan interval. Stratigraphic variations of the stable isotopes are important in the series with lighter δ18O values (>1.5‰) than those of the Neoproterozoic ocean in the SCIc unit. According to regional stratigraphy a temperature effect can be dismissed and a freshwater surface layer is the origin of such negative δ18O values in this unit. The negative δ13C anomaly (-3.5‰ on average) of the Cap Carbonate is similarly to the δ18O values (-6.4‰ on average) in the range of the marine domain during postglacial sea level rise. The sample suite as a whole (SCII and SCIII formations) displays heavier δ18O and δ13C than those of the lower part (SCI unit) of the Schisto-Calcaire Group. The comparison with the Lower Congo (Democratic Republic of Congo) and Nyanga (Gabon) basins shows that the meteoric flushing in SCIc unit of the Schisto-Calcaire Group was regional and not local, and could be derived from a climatic evolution. Although an overall overprint is present, our isotopic relationships argue against overall diagenetic resetting of primary compositions and suggest that with careful examination combined with detailed petrographic analysis general depositional and diagenetic controls can be discerned in oxygen and carbon isotopic data in the Schisto-Calcaire Group.

A biomass representative land cover classification for the Democratic Republic of Congo derived from the Forets D'Afrique Central Evaluee par Teledetection (FACET) data set

NASA Astrophysics Data System (ADS)

Molinario, G.; Hansen, M.; Potapov, P.; Altstatt, A. L.; Justice, C. O.

2012-12-01

The FACET forest cover and forest cover loss 2000-2005-2010 data set has been produced by South Dakota State University, the University of Maryland and the Kinshasa-based Observatoire Satellital des Forets D'Afrique Central (OSFAC) with funding from the USAID Central African Regional Program for the Environment (CARPE). The product is now available or being finalized for the DRC, the ROC and Gabon with plans to complete all Congo Basin countries. While FACET provides unprecedented synoptic detail in the extent of Congo Basin forest and the forest cover loss, additional information is required to stratify land cover into types indicative of biomass content. Analysis of the FACET patterns of deforestation, more detailed remote sensing analysis of biophysical attributes within the FACET land cover classes and GIS-derived classes of degradation obtained through variable distance buffers based on relevant literature and ground truth data are combined with the existing FACET classes to produce a ranking of land cover from low biomass to high biomass for the Democratic Republic of Congo. The resulting classification can be used in all Reduced Emissions from Degradation and Deforestation (REDD) pre-inventory phases when baseline forest cover needs to be known and the location and amount of forest biomass inventory plots needs to be designed. FACET cover loss classes were kept in the classification and can provide the Monitoring, Reporting and Verification tools needed for REDD projects. The project will be demonstrated for the Maringa Lopori Wamba Landscape of the DRC where this work was funded by the African Wildlife Foundation to support the design of a REDD pilot project.

Trace metals and persistent organic pollutants in sediments from river-reservoir systems in Democratic Republic of Congo (DRC): Spatial distribution and potential ecotoxicological effects.

PubMed

Mwanamoki, Paola M; Devarajan, Naresh; Thevenon, Florian; Birane, Niane; de Alencastro, Luiz Felippe; Grandjean, Dominique; Mpiana, Pius T; Prabakar, Kandasamy; Mubedi, Josué I; Kabele, Christophe G; Wildi, Walter; Poté, John

2014-09-01

This paper discusses the occurrence and spatial distribution of metals and persistent organic pollutants (POPs: including organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs), Polybrominated diphenyl ethers (PBDEs), and polycyclic aromatic hydrocarbons (PAHs) in sediments from a river-reservoir system. Surface sediments were sampled from thirteen sites of the Congo River Basin and Lake Ma Vallée, both situated in the vicinity of the capital city Kinshasa (Congo Democratic Republic). Sediment qualities were evaluated using toxicity test based on exposing Ostracods to the sediment samples. The highest metal concentrations were observed in sediments subjected to anthropogenic influences, urban runoff and domestic and industrial wastewaters, discharge into the Congo River basin. Ostracods exposed to the sediments resulted in 100% mortality rates after 6d of incubation, indicating the ultimate toxicity of these sediments as well as potential environmental risks. The POPs and PAHs levels in all sediment samples were low, with maximum concentration found in the sediments (area of pool Malebo): OCP value ranged from 0.02 to 2.50 with ∑OCPs: 3.3μgkg(-1); PCB ranged from 0.07 to 0.99 with Total PCBs (∑7×4.3): 15.31μgkg(-1); PAH value ranged from 0.12 to 9.39 with ∑PAHs: 63.89μgkg(-1). Our results indicate that the deterioration of urban river-reservoir water quality result mainly from urban stormwater runoff, untreated industrial effluents which discharge into the river-reservoirs, human activities and uncontrolled urbanization. This study represents useful tools incorporated to evaluate sediment quality in river-reservoir systems which can be applied to similar aquatic environments. Copyright © 2014 Elsevier Ltd. All rights reserved.

Baseline levels and trophic transfer of persistent organic pollutants in sediments and biota from the Congo River Basin (DR Congo).

PubMed

Verhaert, Vera; Covaci, Adrian; Bouillon, Steven; Abrantes, Katya; Musibono, Dieudonné; Bervoets, Lieven; Verheyen, Erik; Blust, Ronny

2013-09-01

The present study aimed to evaluate the occurrence of persistent organic pollutants (POPs: (PCBs, PBDEs, DDTs, HCHs, CHLs and HCB) in sediments and biota from the middle Congo River Basin (CRB) and to investigate their trophic transfer through the aquatic food web using nitrogen stable isotope ratios. To our knowledge, no data on levels of POPs in sediment and biota from the CRB are present in the literature, and studies on trophic transfer and biomagnification profiles of POPs using δ(15)N are scarce in tropical regions. POP levels in the sediment and biota were low, with exception of total PCB levels found in fish from the Itimbiri River (1.4 to 44ng/g ww). Compared to concentrations found in fish from pristine to relatively industrial developed areas, the ∑PCB levels in fish from the Itimbiri were high, indicating the presence of a local PCB contamination source in this catchment. Based on minimum risk level criteria formulated by ATSDR, the consumption of PCB contaminated fish from the Itimbiri river poses a potential risk for humans. The POP levels in biota were not significantly related to the POP levels in sediments, and the BSAF concept (Biota-Sediment Accumulation Factor) was found to be a poor predictor of the bioavailability and bioaccumulation of environmental pollutants in the present study. With increasing trophic levels, a significant increase in PCB 95, 101, 110, 138, 146, 149, 153, 174, 180 & 187 and p,p'-DDT in Itimbiri and BDE 47 & 99 in Itimbiri, Aruwimi & Lomami river basins was observed. Trophic magnification factors were higher than 1, indicating that biomagnification occurs through the tropical food web. Copyright © 2013 Elsevier Ltd. All rights reserved.

Remotely Sensed Based Lake/Reservoir Routing in Congo River Basin

NASA Astrophysics Data System (ADS)

Raoufi, R.; Beighley, E.; Lee, H.

2017-12-01

Lake and reservoir dynamics can influence local to regional water cycles but are often not well represented in hydrologic models. One challenge that limits their inclusion in models is the need for detailed storage-discharge behavior that can be further complicated in reservoirs where specific operation rules are employed. Here, the Hillslope River Routing (HRR) model is combined with a remotely sensed based Reservoir Routing (RR) method and applied to the Congo River Basin. Given that topographic data are often continuous over the entire terrestrial surface (i.e., does not differentiate between land and open water), the HRR-RR model integrates topographic derived river networks and catchment boundaries (e.g., HydroSHEDs) with water boundary extents (e.g., Global Lakes and Wetlands Database) to develop the computational framework. The catchments bordering lakes and reservoirs are partitioned into water and land portions, where representative flowpath characteristics are determined and vertical water balance and lateral routings is performed separately on each partition based on applicable process models (e.g., open water evaporation vs. evapotranspiration). To enable reservoir routing, remotely sensed water surface elevations and extents are combined to determine the storage change time series. Based on the available time series, representative storage change patterns are determined. Lake/reservoir routing is performed by combining inflows from the HRR-RR model and the representative storage change patterns to determine outflows. In this study, a suite of storage change patterns derived from remotely sensed measurements are determined representative patterns for wet, dry and average conditions. The HRR-RR model dynamically selects and uses the optimal storage change pattern for the routing process based on these hydrologic conditions. The HRR-RR model results are presented to highlight the importance of lake attenuation/routing in the Congo Basin.

Forest Conversion, Agricultural Transitions and the Influence of Multi-scale Market Factors in Southwest Cameroon

NASA Astrophysics Data System (ADS)

Ordway, E.; Lambin, E.; Asner, G. P.

2015-12-01

The changing structure of demand for commodities associated with food security and energy has had a startling impact on land use change in tropical forests in recent decades. Yet, the composition of conversion in the Congo basin remains a major uncertainty, particularly with regards to the scale of drivers of change. Owing to rapid expansion of production globally and longstanding historical production locally in the Congo basin, oil palm offers a lens through which to evaluate local land use decisions across a spectrum of small- to large-scales of production as well as interactions with regional and global supply chains. We examined the effect of global commodity crop expansion on land use change in Southwest Cameroon using a mixed-methods approach to integrate remote sensing, field surveys and socioeconomic data. Southwest Cameroon (2.5 Mha) has a long history of large- and small-scale agriculture, ranging from mixed crop subsistence agriculture to large monocrop plantations of oil palm, cocoa, and rubber. Trends and spatial patterns of forest conversion and agricultural transitions were analyzed from 2000-2015 using satellite imagery. We used economic, demographic and field survey datasets to assess how regional and global market factors and local commodity crop decisions affect land use patterns. Our results show that oil palm is a major commodity crop expanding in this region, and that conversion is occurring primarily through expansion by medium-scale producers and local elites. Results also indicate that global and regional supply chain dynamics influence local land use decision making. This research contributes new information on land use patterns and dynamics in the Congo basin, an understudied region. More specifically, results from this research contribute information on recent trends of oil palm expansion in Cameroon that will be used in national land use planning strategies.

Sediment-hosted stratabound copper assessment of the Neoproterozoic Roan Group, central African copperbelt, Katanga Basin, Democratic Republic of the Congo and Zambia: Chapter T in Global mineral resource assessment

USGS Publications Warehouse

Zientek, Michael L.; Bliss, James D.; Broughton, David W.; Christie, Michael; Denning, Paul; Hayes, Timothy S.; Hitzman, Murray W.; Horton, John D.; Frost-Killian, Susan; Jack, Douglas J.; Master, Sharad; Parks, Heather L.; Taylor, Cliff D.; Wilson, Anna B.; Wintzer, Niki E.; Woodhead, Jon

2014-01-01

This study estimates the location, quality, and quantity of undiscovered copper in stratabound deposits within the Neoproterozoic Roan Group of the Katanga Basin in the Democratic Republic of the Congo and Zambia. The study area encompasses the Central African Copperbelt, the greatest sediment-hosted copper-cobalt province in the world, containing 152 million metric tons of copper in greater than 80 deposits. This study (1) delineates permissive areas (tracts) where undiscovered sediment-hosted stratabound copper deposits may occur within 2 kilometers of the surface, (2) provides a database of known sediment-hosted stratabound copper deposits and prospects, (3) estimates numbers of undiscovered deposits within these permissive tracts at several levels of confidence, and (4) provides probabilistic estimates of amounts of copper and mineralized rock that could be contained in undiscovered deposits within each tract. The assessment, conducted in January 2010 using a three-part form of mineral resource assessment, indicates that a substantial amount of undiscovered copper resources might occur in sediment-hosted stratabound copper deposits within the Roan Group in the Katanga Basin. Monte Carlo simulation results that combine grade and tonnage models with estimates of undiscovered deposits indicate that the mean estimate of undiscovered copper in the study area is 168 million metric tons, which is slightly greater than the known resources at 152 million metric tons. Furthermore, significant value can be expected from associated metals, particularly cobalt. Tracts in the Democratic Republic of the Congo (DRC) have potential to contain near-surface, undiscovered deposits. Monte Carlo simulation results indicate a mean value of 37 million metric tons of undiscovered copper may be present in significant prospects.

Climate change and forest communities: prospects for building institutional adaptive capacity in the Congo Basin forests.

PubMed

Brown, H Carolyn Peach; Smit, Barry; Somorin, Olufunso A; Sonwa, Denis J; Nkem, Johnson Ndi

2014-10-01

Tropical forests are vulnerable to climate-change representing a risk for indigenous peoples and forest-dependent communities. Mechanisms to conserve the forest, such as REDD+, could assist in the mitigation of climate change, reduce vulnerability, and enable people to adapt. Ninety-eight interviews were conducted in three countries containing the Congo Basin forest, Cameroon, CAR, and DRC, to investigate perceptions of decision-makers within, and responses of the institutions of the state, private sector, and civil society to the challenges of climate change. Results indicate that while decision-makers' awareness of climate change is high, direct institutional action is at an early stage. Adaptive capacity is currently low, but it could be enhanced with further development of institutional linkages and increased coordination of multilevel responses across all institutions and with local people. It is important to build networks with forest-dependent stakeholders at the local level, who can contribute knowledge that will build overall institutional adaptive capacity.

Trace metal distributions in the sediments from river-reservoir systems: case of the Congo River and Lake Ma Vallée, Kinshasa (Democratic Republic of Congo).

PubMed

Mwanamoki, Paola M; Devarajan, Naresh; Niane, Birane; Ngelinkoto, Patience; Thevenon, Florian; Nlandu, José W; Mpiana, Pius T; Prabakar, Kandasamy; Mubedi, Josué I; Kabele, Christophe G; Wildi, Walter; Poté, John

2015-01-01

The contamination of drinking water resources by toxic metals is a major problem in many parts of the world, particularly in dense populated areas of developing countries that lack wastewater treatment facilities. The present study characterizes the recent evolution with time of some contaminants deposited in the Congo River and Lake Ma Vallée, both located in the vicinity of the large city of Kinshasa, capital of Democratic Republic of Congo (DRC). Physicochemical parameters including grain size distribution, organic matter and trace element concentrations were measured in sediment cores sampled from Congo River (n = 3) and Lake Ma Vallée (n = 2). The maximum concentration of trace elements in sediment profiles was found in the samples from the sites of Pool Malebo, with the values of 107.2, 111.7, 88.6, 39.3, 15.4, 6.1 and 4.7 mg kg(-1) for Cr, Ni, Zn, Cu, Pb, As and Hg, respectively. This site, which is characterized by intense human activities, is especially well known for the construction of numerous boats that are used for regular navigation on Congo River. Concerning Lake Ma Vallée, the concentration of all metals are generally low, with maximum values of 26.3, 53.6, 16.1, 15.3, 6.5 and 1.8 mg kg(-1) for Cr, Ni, Zn, Cu, Pb and As, respectively. However, the comparison of the metal profiles retrieved from the different sampled cores also reveals specific variations. The results of this study point out the sediment pollution by toxic metals in the Congo River Basin. This research presents useful tools for the evaluation of sediment contamination of river-reservoir systems.

Effects of Epidemic Diseases on the Distribution of Bonobos

PubMed Central

Inogwabini, Bila-Isia; Leader-Williams, Nigel

2012-01-01

This study examined how outbreaks and the occurrence of Anthrax, Ebola, Monkeypox and Trypanosomiasis may differentially affect the distribution of bonobos (Pan paniscus). Using a combination of mapping, Jaccard overlapping coefficients and binary regressions, the study determined how each disease correlated with the extent of occurrence of, and the areas occupied by, bonobos. Anthrax has only been reported to occur outside the range of bonobos and so was not considered further. Ebola, Monkeypox and Trypanosomiasis were each reported within the area of occupancy of bonobos. Their respective overlap coefficients were: J = 0.10; Qα = 0.05 = 2.00 (odds ratios = 0.0001, 95% CI = 0.0057; Z = −19.41, significant) for Ebola; J = 1.00; Qα = 0.05 = 24.0 (odds ratios = 1.504, 95% CI = 0.5066–2.6122) for Monkeypox; and, J = 0.33; Qα = 0.05 = 11.5 (Z = 1.14, significant) for Trypanosomiasis. There were significant relationships for the presence and absence of Monkeypox and Trypanosomiasis and the known extent of occurrence of bonobos, based on the equations y = 0.2368Ln(x)+0.8006 (R2 = 0.9772) and y = −0.2942Ln(x)+0.7155 (R2 = 0.698), respectively. The positive relationship suggested that bonobos tolerated the presence of Monkeypox. In contrast, the significant negative coefficient suggested that bonobos were absent in areas where Trypanosomiasis is endemic. Our results suggest that large rivers may have prevented Ebola from spreading into the range of bonobos. Meanwhile, Trypanosomiasis has been recorded among humans within the area of occurrence of bonobos, and appears the most important disease in shaping the area of occupancy of bonobos within their overall extent of occupancy. PMID:23251431

Estimation of surface water storage in the Congo Basin

NASA Astrophysics Data System (ADS)

O'Loughlin, F.; Neal, J. C.; Schumann, G.; Beighley, E.; Bates, P. D.

2015-12-01

For many large river basins, especially in Africa, the lack of access to in-situ measurements, and the large areas involved, make modelling of water storage and runoff difficult. However, remote sensing datasets are useful alternative sources of information, which overcome these issues. In this study, we focus on the Congo Basin and, in particular, the cuvette central. Despite being the second largest river basin on earth and containing a large percentage of the world's tropical wetlands and forest, little is known about this basin's hydrology. Combining discharge estimates from in-situ measurements and outputs from a hydrological model, we build the first large-scale hydrodynamic model for this region to estimate the volume of water stored in the corresponding floodplains and to investigate how important these floodplains are to the behaviour of the overall system. This hydrodynamic model covers an area over 1.6 million square kilometres and 13 thousand kilometres of rivers and is calibrated to water surface heights at 33 virtual gauging stations obtained from ESA's Envisat satellite. Our results show that the use of different sources of discharge estimations and calibration via Envisat observations can produce accurate water levels and downstream discharges. Our model produced un-biased (bias =-0.08 m), sub-metre Root Mean Square Error (RMSE =0.862 m) with a Nash-Sutcliffe efficiency greater than 80% (NSE =0.81). The spatial-temporal variations in our simulated inundated areas are consistent with the pattern obtained from satellites. Overall, we find a high correlation coefficient (R =0.88) between our modelled inundated areas and those estimated from satellites.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vernet, R.

The Bas Congo basin extends from Gabon to Angola and is a prolific oil province where both pre-salt and post salt sources and reservoirs have been found. In the northern part of the basin referred to as the Congo coastal basin, the proven petroleum system is more specific: mature source rocks are found only in pre-salt series whereas by contrast 99 % proven hydrocarbon reserves am located in post-salt traps. Such a system is controlled by the following factors: Source rocks are mostly organic rich shales deposited in a restricted environment developed in a rift prior to the Atlantic Oceanmore » opening; Migration from pre-salt sources to post-salt traps is finalized by local discontinuities of the regional salt layer acting otherwise as a tight seal; Post-salt reservoirs are either carbonates or sands desposited in the evolutive shelf margin developped during Upper Cretaceous; Geometric traps are linked to salt tectonics (mostly turtle-shaped structures); Regional shaly seals are related to transgressive shales best developped during high rise sea level time interval. Stratigraphically, the age of hydrocarbon fields trends are younger and younger from West to East: lower Albian in Nkossa, Upper Albian and lower Cenomanian in Likouala, Yanga, Sendji, Upper Cenomanian in Tchibouela, Turonian in Tchendo, Turanian and Senonian in Emeraude.« less

Deletion of the monkeypox virus inhibitor of complement enzymes locus impacts the adaptive immune response to monkeypox virus in a nonhuman primate model of infection.

PubMed

Estep, Ryan D; Messaoudi, Ilhem; O'Connor, Megan A; Li, Helen; Sprague, Jerald; Barron, Alexander; Engelmann, Flora; Yen, Bonnie; Powers, Michael F; Jones, John M; Robinson, Bridget A; Orzechowska, Beata U; Manoharan, Minsha; Legasse, Alfred; Planer, Shannon; Wilk, Jennifer; Axthelm, Michael K; Wong, Scott W

2011-09-01

Monkeypox virus (MPXV) is an orthopoxvirus closely related to variola virus, the causative agent of smallpox. Human MPXV infection results in a disease that is similar to smallpox and can also be fatal. Two clades of MPXV have been identified, with viruses of the central African clade displaying more pathogenic properties than those within the west African clade. The monkeypox inhibitor of complement enzymes (MOPICE), which is not expressed by viruses of the west African clade, has been hypothesized to be a main virulence factor responsible for increased pathogenic properties of central African strains of MPXV. To gain a better understanding of the role of MOPICE during MPXV-mediated disease, we compared the host adaptive immune response and disease severity following intrabronchial infection with MPXV-Zaire (n = 4), or a recombinant MPXV-Zaire (n = 4) lacking expression of MOPICE in rhesus macaques (RM). Data presented here demonstrate that infection of RM with MPXV leads to significant viral replication in the peripheral blood and lungs and results in the induction of a robust and sustained adaptive immune response against the virus. More importantly, we show that the loss of MOPICE expression results in enhanced viral replication in vivo, as well as a dampened adaptive immune response against MPXV. Taken together, these findings suggest that MOPICE modulates the anti-MPXV immune response and that this protein is not the sole virulence factor of the central African clade of MPXV.

Resilience landscapes for Congo basin rainforests vs. climate and management impacts

NASA Astrophysics Data System (ADS)

Pietsch, Stephan Alexander; Gautam, Sishir; Elias Bednar, Johannes; Stanzl, Patrick; Mosnier, Aline; Obersteiner, Michael

2015-04-01

Past climate change caused severe disturbances of the Central African rainforest belt, with forest fragmentation and re-expansion due to drier and wetter climate conditions. Besides climate, human induced forest degradation affected biodiversity, structure and carbon storage of Congo basin rainforests. Information on climatically stable, mature rainforest, unaffected by human induced disturbances, provides means of assessing the impact of forest degradation and may serve as benchmarks of carbon carrying capacity over regions with similar site and climate conditions. BioGeoChemical (BGC) ecosystem models explicitly consider the impacts of site and climate conditions and may assess benchmark levels over regions devoid of undisturbed conditions. We will present a BGC-model validation for the Western Congolian Lowland Rainforest (WCLRF) using field data from a recently confirmed forest refuge, show model - data comparisons for disturbed und undisturbed forests under different site and climate conditions as well as for sites with repeated assessment of biodiversity and standing biomass during recovery from intensive exploitation. We will present climatic thresholds for WCLRF stability, and construct resilience landscapes for current day conditions vs. climate and management impacts.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dupre, B.; Rousseau, D.; Gaillardet, J.

The Congo river Basin is the second largest drainage basin in the world, after the Amazon. The materials carried by its main rivers provide the opportunity to study the products of denudation of a large fraction of the upper continental crust of the African continent. This paper presents the chemical composition of the different phases carried in the Congo rivers and is followed by a companion paper, devoted to the modelling of major and trace elements. The Congo river between Bangui and Brazzaville as well as its main tributaries, including a few organic-rich rivers, also called Black Rivers, were sampledmore » during the 1989 high water stage. The three main phases (suspended load, dissolved load, and bedload) were analysed for twenty-five major and trace elements. Concentrations normalized to the upper continental crust show that in each river, suspended sediments and dissolved load are chemical complements for the most soluble elements (Ca, Na, Sr, K, Ba, Rb, and U). While these elements are enriched in the dissolved loads, they are considerably depleted in the corresponding suspended sediments. This is consistent with their high mobility during weathering. Another type of complementarity is observed for Zr and Hf between suspended sediments and bedload, related to the differential velocity of suspended sediments and zircons which are concentrated in bedloads. Compared to other rivers, absolute dissolved concentrations of Ca, Na, Sr, K, Ba, Rb, and U are remarkably low. Surprisingly, high dissolved concentrations are found in the Congo waters for other trace elements (e.g., REEs), especially in the Black rivers. On a world scale, these concentrations are among the highest measured in rivers and are shown to be pH dependent for a number of dissolved trace elements. The dissolved loads are systematically normalized to the suspended loads for each river, in order to remove the variations of the element abundances owing to source rock variations.« less

Hydraulic characterization of the middle reach of the Congo River

NASA Astrophysics Data System (ADS)

O'Loughlin, F.; Trigg, M. A.; Schumann, G. J.-P.; Bates, P. D.

2013-08-01

The middle reach of the Congo remains one of the most difficult places to access, with ongoing conflicts and a lack of infrastructure. This has resulted in the Congo being perhaps the least understood large river hydraulically, particularly compared to the Amazon, Nile, or Mississippi. Globally the Congo River is important; it is the largest river in Africa and the basin contains some of the largest areas of tropical forests and wetlands in the world, which are important to both the global carbon and methane cycles. This study produced the first detailed hydraulic characterization of the middle reach, utilizing mostly remotely sensed data sets. Using Landsat imagery, a 30 m resolution water-mask was created for the middle reach, from which effective river widths and the number of channels and islands were determined. Water surface slopes were determined using ICESat observations for three different periods during the annual flood pulse, and while the overall slope calculated was similar to previous estimates, greater spatial variability was identified. We find that the water surface slope varies markedly in space but relatively little in time and that this appears to contrast with the Amazon where previous studies indicate that time and spatial variations are of equal magnitude. Five key hydraulic constraints were also identified, which play an important role in the overall dynamics of the Congo. Finally, backwater lengths were approximated for four of these constraints, with the results showing that at high water, over a third of the middle reach is affected by backwater effects.

Further assessment of Monkeypox Virus infection in Gambian pouched rats (Cricetomys gambianus) using in vivo bioluminescent imaging

USGS Publications Warehouse

Falendysz, Elizabeth; Lopera, Juan G.; Faye Lorenzsonn,; Salzer, Johanna S.; Hutson, Christina L.; Doty, Jeffrey; Gallardo-Romero, Nadia; Carroll, Darin S.; Osorio, Jorge E.; Rocke, Tonie E.

2015-01-01

Monkeypox is a zoonosis clinically similar to smallpox in humans. Recent evidence has shown a potential risk of increased incidence in central Africa. Despite attempts to isolate the virus from wild rodents and other small mammals, no reservoir host has been identified. In 2003,Monkeypox virus (MPXV) was accidentally introduced into the U.S. via the pet trade and was associated with the Gambian pouched rat (Cricetomys gambianus). Therefore, we investigated the potential reservoir competence of the Gambian pouched rat for MPXV by utilizing a combination of in vivo and in vitro methods. We inoculated three animals by the intradermal route and three animals by the intranasal route, with one mock-infected control for each route. Bioluminescent imaging (BLI) was used to track replicating virus in infected animals and virological assays (e.g. real time PCR, cell culture) were used to determine viral load in blood, urine, ocular, nasal, oral, and rectal swabs. Intradermal inoculation resulted in clinical signs of monkeypox infection in two of three animals. One severely ill animal was euthanized and the other affected animal recovered. In contrast, intranasal inoculation resulted in subclinical infection in all three animals. All animals, regardless of apparent or inapparent infection, shed virus in oral and nasal secretions. Additionally, BLI identified viral replication in the skin without grossly visible lesions. These results suggest that Gambian pouched rats may play an important role in transmission of the virus to humans, as they are hunted for consumption and it is possible for MPXV-infected pouched rats to shed infectious virus without displaying overt clinical signs.

Deletion of the Monkeypox Virus Inhibitor of Complement Enzymes Locus Impacts the Adaptive Immune Response to Monkeypox Virus in a Nonhuman Primate Model of Infection ▿ §

PubMed Central

Estep, Ryan D.; Messaoudi, Ilhem; O'Connor, Megan A.; Li, Helen; Sprague, Jerald; Barron, Alexander; Engelmann, Flora; Yen, Bonnie; Powers, Michael F.; Jones, John M.; Robinson, Bridget A.; Orzechowska, Beata U.; Manoharan, Minsha; Legasse, Alfred; Planer, Shannon; Wilk, Jennifer; Axthelm, Michael K.; Wong, Scott W.

2011-01-01

Monkeypox virus (MPXV) is an orthopoxvirus closely related to variola virus, the causative agent of smallpox. Human MPXV infection results in a disease that is similar to smallpox and can also be fatal. Two clades of MPXV have been identified, with viruses of the central African clade displaying more pathogenic properties than those within the west African clade. The monkeypox inhibitor of complement enzymes (MOPICE), which is not expressed by viruses of the west African clade, has been hypothesized to be a main virulence factor responsible for increased pathogenic properties of central African strains of MPXV. To gain a better understanding of the role of MOPICE during MPXV-mediated disease, we compared the host adaptive immune response and disease severity following intrabronchial infection with MPXV-Zaire (n = 4), or a recombinant MPXV-Zaire (n = 4) lacking expression of MOPICE in rhesus macaques (RM). Data presented here demonstrate that infection of RM with MPXV leads to significant viral replication in the peripheral blood and lungs and results in the induction of a robust and sustained adaptive immune response against the virus. More importantly, we show that the loss of MOPICE expression results in enhanced viral replication in vivo, as well as a dampened adaptive immune response against MPXV. Taken together, these findings suggest that MOPICE modulates the anti-MPXV immune response and that this protein is not the sole virulence factor of the central African clade of MPXV. PMID:21752919

Characterizing the Responses of Land Surface Phenology to the Rainy Season in the Congo Basin

NASA Astrophysics Data System (ADS)

Yan, D.; Zhang, X.; Yu, Y.; Guo, W.

2016-12-01

The most pronounced climate changes across the Congo Basin are predicted to be the changes in the timing and amount of rainfall in the coming decades. It is expected to alter a significant shift in land surface phenology (LSP), so that an understanding of its responses to the rainy season can benefit the predictions of changes in the Congolese ecosystem under future climate change scenarios. However, quantitative analyses has not been performed to investigate the relationship between LSP and the rainy season in the Congo Basin. Based on 30-minute observations acquired by the Spinning Enhanced Visible and Infrared Imager (SEVIRI) onboard the METEOSAT Second Generation series of geostationary satellites, we generated a time series of three-day angularly corrected Two-band Enhanced Vegetation Index (EVI2) between 2006 and 2013. We then reconstructed EVI2 temporal trajectories and retrieved the timings and magnitudes of LSP using the hybrid piecewise logistic model. We further associated the phenological timings and magnitudes with those of the rainy seasons derived from the three-hourly rainfall rate measurements provided by the Tropical Rainfall Measurement Mission Product 3B42. Finally, we investigated the impacts of tree cover on the timing discrepancy between LSP and the rainy season. Results show that LSP was strongly associated with the rainy season. Specifically, the SEVIRI EVI2 time series reveals that two annual canopy greenness cycles (CGC) occur in the Congolese rainforests whereas a single annual CGC with strong seasonal amplitude was identified for other land cover types. The spatial shifts in CGC timings closely follow those of the rainy season controlled by the seasonal migration of the Intertropical Convergence Zone. However, the tree cover controls the timing discrepancy between LSP and the rainy season. The accumulated vegetation greenness during a CGC shows a strong dependence on the total rainfall received.

Parameterisation of Biome BGC to assess forest ecosystems in Africa

NASA Astrophysics Data System (ADS)

Gautam, Sishir; Pietsch, Stephan A.

2010-05-01

African forest ecosystems are an important environmental and economic resource. Several studies show that tropical forests are critical to society as economic, environmental and societal resources. Tropical forests are carbon dense and thus play a key role in climate change mitigation. Unfortunately, the response of tropical forests to environmental change is largely unknown owing to insufficient spatially extensive observations. Developing regions like Africa where records of forest management for long periods are unavailable the process-based ecosystem simulation model - BIOME BGC could be a suitable tool to explain forest ecosystem dynamics. This ecosystem simulation model uses descriptive input parameters to establish the physiology, biochemistry, structure, and allocation patterns within vegetation functional types, or biomes. Undocumented parameters for larger-resolution simulations are currently the major limitations to regional modelling in African forest ecosystems. This study was conducted to document input parameters for BIOME-BGC for major natural tropical forests in the Congo basin. Based on available literature and field measurements updated values for turnover and mortality, allometry, carbon to nitrogen ratios, allocation of plant material to labile, cellulose, and lignin pools, tree morphology and other relevant factors were assigned. Daily climate input data for the model applications were generated using the statistical weather generator MarkSim. The forest was inventoried at various sites and soil samples of corresponding stands across Gabon were collected. Carbon and nitrogen in the collected soil samples were determined from soil analysis. The observed tree volume, soil carbon and soil nitrogen were then compared with the simulated model outputs to evaluate the model performance. Furthermore, the simulation using Congo Basin specific parameters and generalised BIOME BGC parameters for tropical evergreen broadleaved tree species were also executed and the simulated results compared. Once the model was optimised for forests in the Congo basin it was validated against observed tree volume, soil carbon and soil nitrogen from a set of independent plots.

A Lagrangian perspective of the hydrological cycle in the Congo River basin

NASA Astrophysics Data System (ADS)

Sorí, Rogert; Nieto, Raquel; Vicente-Serrano, Sergio M.; Drumond, Anita; Gimeno, Luis

2017-08-01

The Lagrangian model FLEXPART is used to identify the moisture sources of the Congo River basin (CRB) and investigate their role in the hydrological cycle. This model allows us to track atmospheric parcels while calculating changes in the specific humidity through the budget of evaporation minus precipitation. This method permits the annual-scale identification of five continental and four oceanic principal regions that provide moisture to the CRB from both hemispheres over the course of the year. The most important is the CRB, which provides more than 50 % of the total atmospheric moisture contribution to precipitation over itself. Additionally, both the land that extends to the east of the CRB and the eastern equatorial South Atlantic Ocean are very important sources, while the Red Sea source is merely important in the (E - P) budget over the CRB despite its high evaporation rate. The moisture-sink patterns over the CRB in air masses that were tracked forward in time from all the sources follow the latitudinal rainfall migration and are mostly highly correlated with the pattern of the precipitation rate, ensuring a link between them. In wet (dry) years, the contribution of moisture to precipitation from the CRB over itself increases (decreases). Despite the enhanced evaporative conditions over the basin during dry years, the vertically integrated moisture flux (VIMF) divergence inhibits precipitation and suggests the transport of moisture from the CRB to remote regions.

Collection and Utilization of Animal Carcasses Associated with zoonotic Disease in Tshuapa District, the Democratic Republic of the Congo, 2012.

PubMed

Monroe, Benjamin P; Doty, Jeffrey B; Moses, Cynthia; Ibata, Saturnin; Reynolds, Mary; Carroll, Darin

2015-07-01

The collection and consumption of animal carcasses is a common activity in forested areas of the Congo River basin and creates sustainability, conservation, and health concerns. Residents of the Tshuapa District reported collecting the remains of 5,878 animals from >30 species when surveyed about their wildlife consumption habits. Carcasses were discovered in varying degrees of decomposition and were often consumed at home or sold in local markets. The most commonly collected animals were Cricetomys gambianus (Northern giant pouched rat), Cercopithecus ascanius (red-tailed monkey), and Heliosciurus rufobrachium (red-legged sun squirrel). Many of the species recorded may be hosts of zoonotic pathogens, creating concern for spillover events.

[Identification of human pathogenic variola and monkeypox viruses by real-time polymerase chain reaction].

PubMed

Kostina, E V; Gavrilova, E V; Riabinin, V A; Shchelkunov, S N; Siniakov, A N

2009-01-01

A kit of specific oligonucleotide primers and hybridization probes has been proposed to detect orthopoxviruses (OPV) and to discriminate human pathogenic viruses, such as variola virus and monkey virus by real-time polymerase chain reaction (PCR). For real-time PCR, the following pairs of fluorophore and a fluorescence quencher were used: TAMRA-BHQ2 for genus-specific probes and FAM-BHQ1 for species-specific ones (variola virus, monkeypox virus, ectomelia virus). The specificity of this assay was tested on 38 strains of 6 OPV species and it was 100%.

Weather For Aircrews - Air Force Handbook 11-203, Volume 1

DTIC Science & Technology

1997-03-01

I S-X. Aurora Borealis...combinations of these factors produce tropical weather ranging from the hot, humid climate of the Amazon and Congo river basins, to the arid Sahara Desert... Aurora . Disturbances on the sun spew out bursts of energy particles, a portion of which eventually reach the earth. The high-energy particles strike

Neogene Rift Propagation of the East African Rift System (EARS) into Central Africa and its Implications: Tectonic, Topographic and Geomorphic Impacts of the Luangwa and Luapula Rift Valleys on the Upper Congo Drainage Basin, Lake Bangweulu Wetlands and the Development of the Diffuse Southwestern Tip of the EARS.

NASA Astrophysics Data System (ADS)

Daly, M. C.; Watts, A. B.

2017-12-01

Integration of geomorphology, seismic reflection and gravity data, seismicity, DEM analysis and modelling defines a zone of NE/SW trending rifts extending into Central and SW Africa, orthogonal to the conventionally defined East African Rift System (EARS). These large-scale tectonic features have a relatively low level of seismicity and volcanism compared to the EARS, yet they generate significant topography and control the upper Congo drainage basin. They may also represent the beginning of an active but diffuse plate boundary developing to the southwest across Central Africa. The dominant feature of this broad zone is the Luangwa Rift Valley of eastern Zambia. Seismic reflection data show the Luangwa Rift developed as a thick ( 5km) Permo-Triassic basin. Inverted in the Mesozoic, it then experienced major Neogene extensional reactivation. The latter resulted in today's major border faults of varying polarity, with fault plane escarpments of up to 1000m, and associated rift flank uplifts that elevate the Central African plateau surface by 200 m. Late Miocene alluvial fans indicate a minimum age for the initiation of reactivation. Although having similar structural features to the EARS, the Luangwa Rift has a lower level of active seismicity and volcanism. 400 km northwest of the Luangwa, the north/south Luapula rift valley passes into the NE trending Mweru and Mweru Wantipa rift lakes. Pronounced border faults and fault terraces mark the NW and SE margins of these shallow lakes. Between the Luangwa and Luapula rift valleys lies the extensive upper Congo drainage basin of the Chambeshi river and the Lake Bangweulu wetlands. DEM mapping of topography from the Luangwa rift to the Luapula-Mweru Wantipa rift shows a low amplitude, large wavelength flexure of the Central African plateau surface compatible with an effective elastic thickness of 35 km. This regional warping controls the location and shape of the Chambeshi drainage basin and the Lake Bangweulu Wetlands. These results show Neogene rift valleys are active to the southwest of the EARS and are controlling the present-day continental drainage system of Central Africa. They also define a diffuse, divergent plate boundary between the Nubian Plate and an ill-defined southern African Plate that appears to exploit a zone of crustal anisotropy and thinner lithosphere.

Inorganic carbon speciation and fluxes in the Congo River

NASA Astrophysics Data System (ADS)

Wang, Zhaohui Aleck; Bienvenu, Dinga Jean; Mann, Paul J.; Hoering, Katherine A.; Poulsen, John R.; Spencer, Robert G. M.; Holmes, Robert M.

2013-02-01

Seasonal variations in inorganic carbon chemistry and associated fluxes from the Congo River were investigated at Brazzaville-Kinshasa. Small seasonal variation in dissolved inorganic carbon (DIC) was found in contrast with discharge-correlated changes in pH, total alkalinity (TA), carbonate species, and dissolved organic carbon (DOC). DIC was almost always greater than TA due to the importance of CO2*, the sum of dissolved CO2 and carbonic acid, as a result of low pH. Organic acids in DOC contributed 11-61% of TA and had a strong titration effect on water pH and carbonate speciation. The CO2* and bicarbonate fluxes accounted for ~57% and 43% of the DIC flux, respectively. Congo River surface water released CO2 at a rate of ~109 mol m-2 yr-1. The basin-wide DIC yield was ~8.84 × 104 mol km-2 yr-1. The discharge normalized DIC flux to the ocean amounted to 3.11 × 1011 mol yr-1. The DOC titration effect on the inorganic carbon system may also be important on a global scale for regulating carbon fluxes in rivers.

The mitochondrial ancestor of bonobos and the origin of their major haplogroups.

PubMed

Takemoto, Hiroyuki; Kawamoto, Yoshi; Higuchi, Shoko; Makinose, Emiko; Hart, John A; Hart, Térese B; Sakamaki, Tetsuya; Tokuyama, Nahoko; Reinartz, Gay E; Guislain, Patrick; Dupain, Jef; Cobden, Amy K; Mulavwa, Mbangi N; Yangozene, Kumugo; Darroze, Serge; Devos, Céline; Furuichi, Takeshi

2017-01-01

We report here where the most recent common ancestor (MRCA) of bonobos (Pan paniscus) ranged and how they dispersed throughout their current habitat. Mitochondrial DNA (mtDNA) molecular dating to analyze the time to MRCA (TMRCA) and the major mtDNA haplogroups of wild bonobos were performed using new estimations of divergence time of bonobos from other Pan species to investigate the dispersal routes of bonobos over the forest area of the Congo River's left bank. The TMRCA of bonobos was estimated to be 0.64 or 0.95 million years ago (Ma). Six major haplogroups had very old origins of 0.38 Ma or older. The reconstruction of the ancestral area revealed the mitochondrial ancestor of the bonobo populations ranged in the eastern area of the current bonobos' habitat. The haplogroups may have been formed from either the riparian forests along the Congo River or the center of the southern Congo Basin. Fragmentation of the forest refugia during the cooler periods may have greatly affected the formation of the genetic structure of bonobo populations.

The mitochondrial ancestor of bonobos and the origin of their major haplogroups

PubMed Central

Takemoto, Hiroyuki; Kawamoto, Yoshi; Higuchi, Shoko; Makinose, Emiko; Furuichi, Takeshi

2017-01-01

We report here where the most recent common ancestor (MRCA) of bonobos (Pan paniscus) ranged and how they dispersed throughout their current habitat. Mitochondrial DNA (mtDNA) molecular dating to analyze the time to MRCA (TMRCA) and the major mtDNA haplogroups of wild bonobos were performed using new estimations of divergence time of bonobos from other Pan species to investigate the dispersal routes of bonobos over the forest area of the Congo River’s left bank. The TMRCA of bonobos was estimated to be 0.64 or 0.95 million years ago (Ma). Six major haplogroups had very old origins of 0.38 Ma or older. The reconstruction of the ancestral area revealed the mitochondrial ancestor of the bonobo populations ranged in the eastern area of the current bonobos’ habitat. The haplogroups may have been formed from either the riparian forests along the Congo River or the center of the southern Congo Basin. Fragmentation of the forest refugia during the cooler periods may have greatly affected the formation of the genetic structure of bonobo populations. PMID:28467422

Comparison of Subsidence Rates for Conjugate Margins of the Equatorial and Northern South Atlantic Ocean as A First-Order Constraint on Symmetry of Underlying, Early Rift Structures

NASA Astrophysics Data System (ADS)

Zavala, O.

2017-12-01

We compared subsidence histories from wells into Cretaceous-Cenozoic conjugate margins in the Equatorial and northern South Atlantic as a first-order constraint on whether rifting occurred in a symmetrical, pure shear mode, or whether rifting occurred in an asymmetrical, simple shear mode. For the pure shear mode of rifting, the prediction is for longterm subsidence on both conjugate margins to be similar and reflective of underlying, rift symmetry; for the simple shear mode of rifting, the prediction is that subsidence above the more thinned and wider, lower plate margin is greater than subsidence above the less thinned and more narrow, upper plate margin. A major caveat of this approach is that subsidence variations can be affected by other external factors that include increased sedimentation related to local deltas and structural or hotspot-related uplifts of coastal areas. In the northern Equatorial Atlantic, the longterm subsidence rate for the Guyana basin of northeastern South America of 18.52 m/Ma is less that of the Senegal area of west Africa of 54 m/Ma suggestive of an upper plate to the west and lower plate to the east. Moving southwards, the Potiguar basin of northern Brazil of 23 m/Ma is roughly the same as the Keta-Togo-Benin-Cote d'Ivoire basins of west Africa (21 m/Ma) and suggestive of an underlying rift symmetry. The Bahia Norte-Reconcavo-Sergipe-Alogoas basins of Brazil are less (28 m/Ma) than the Gabon basin (57 m/Ma) of west Africa suggesitive of an lower plate to the east and an upper plate to the west. The Bahia Sul-Espirito Santo basins of Brazil are less (20 m/Ma) than the Lower Congo basin (45 m/Ma) although the latter area includes the localized influence of the Congo delta. We compare additional evidence such as seismic reflection and refraction data and gravity modeling to the predictions of the subsidence values.

State and evolution of the African rainforests between 1990 and 2010

PubMed Central

Mayaux, Philippe; Pekel, Jean-François; Desclée, Baudouin; Donnay, François; Lupi, Andrea; Achard, Frédéric; Clerici, Marco; Bodart, Catherine; Brink, Andreas; Nasi, Robert; Belward, Alan

2013-01-01

This paper presents a map of Africa's rainforests for 2005. Derived from moderate resolution imaging spectroradiometer data at a spatial resolution of 250 m and with an overall accuracy of 84%, this map provides new levels of spatial and thematic detail. The map is accompanied by measurements of deforestation between 1990, 2000 and 2010 for West Africa, Central Africa and Madagascar derived from a systematic sample of Landsat images—imagery from equivalent platforms is used to fill gaps in the Landsat record. Net deforestation is estimated at 0.28% yr−1 for the period 1990–2000 and 0.14% yr−1 for the period 2000–2010. West Africa and Madagascar exhibit a much higher deforestation rate than the Congo Basin, for example, three times higher for West Africa and nine times higher for Madagascar. Analysis of variance over the Congo Basin is then used to show that expanding agriculture and increasing fuelwood demands are key drivers of deforestation in the region, whereas well-controlled timber exploitation programmes have little or no direct influence on forest-cover reduction at present. Rural and urban population concentrations and fluxes are also identified as strong underlying causes of deforestation in this study. PMID:23878331

The national determinants of deforestation in sub-Saharan Africa.

PubMed

Rudel, Thomas K

2013-01-01

For decades, the dynamics of tropical deforestation in sub-Saharan Africa (SSA) have defied easy explanation. The rates of deforestation have been lower than elsewhere in the tropics, and the driving forces evident in other places, government new land settlement schemes and industrialized agriculture, have largely been absent in SSA. The context and causes for African deforestation become clearer through an analysis of new, national-level data on forest cover change for SSA countries for the 2000-2005 period. The recent dynamic in SSA varies from dry to wet biomes. Deforestation occurred at faster rates in nations with predominantly dry forests. The wetter Congo basin countries had lower rates of deforestation, in part because tax receipts from oil and mineral industries in this region spurred rural to urban migration, declines in agriculture and increased imports of cereals from abroad. In this respect, the Congo basin countries may be experiencing an oil and mineral fuelled forest transition. Small farmers play a more important role in African deforestation than they do in southeast Asia and Latin America, in part because small-scale agriculture remains one of the few livelihoods open to rural peoples.

REDD+ projects in the Democratic Republic of Congo: impacts on future emissions, income and biodiversity

NASA Astrophysics Data System (ADS)

Mosnier, Aline; Bocqueho, Geraldine; Mant, Rebecca; Obersteiner, Michael; Havlik, Petr; Kapos, Val; Fritz, Steffen; Botrill, Leo

2014-05-01

The Democratic Republic of Congo (DRC) encompasses a large rainforest area which has been rather preserved up to now. However, pressure on the forests is increasing with high population growth, transition toward political stability and the abundance of minerals in the country. REDD+ is a developing mechanism under the UNFCCC that aims to support developing countries that want to make efforts to reduce their emissions from deforestation and forest degradation. The REDD+ strategy in DRC combines an independent national fund and independent REDD+ projects at the local level that are at the initial stage of implementation. The objective of this paper is to assess i) emissions reduction due to the implementation of the REDD+ pilot projects taking into account potential leakage and ii) potential co-benefits of REDD+ pilot projects in terms of biodiversity and rural income by 2030. We use the land use economic model CongoBIOM adapted from GLOBIOM which represents land-based activities and land use changes at a 50x50km resolution level. It includes domestic and international demand for agricultural products, fuel wood and minerals which are the main deforestation drivers in the Congo Basin region. Finally, we run a sensitivity analysis on emissions from land use change according to three different above and below ground living biomass estimates: downscaled FAO, NASA and WHRC.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mello, M.R.; Soldan, A.L.; Maxwell, J.R.

A geochemical and biological marker investigation of a variety of oils from offshore Brazil and west Africa, ranging in age from Lower Cretaceous to Tertiary, has been done, with the following aims: (1) assessing the depositional environment of source rocks, (2) correlating the reservoired oils, (3) comparing the Brazilian oils with their west African counterparts. The approach was based in stable isotope data; bulk, elemental, and hydrous pyrolysis results; and molecular studies involving quantitative geological marker investigations of alkanes using GC-MS and GC-MS-MS. The results reveal similarities between groups of oils from each side of the Atlantic and suggest anmore » origin from source rocks deposited in five types of depositional environment: lacustrine fresh water, lacustrine saline water, marine evaporitic/carbonate, restricted marine anoxic, and marine deltaic. In west Africa, the Upper Cretaceous marine anoxic succession (Cenomanian-Santonian) appears to be a major oil producer, but in Brazil it is generally immature. The Brazilian offshore oils have arisen mainly from the pre-salt sequence, whereas the African oils show a balance between origins from the pre-salt and marine sequences. The integration of the geochemical and geological data indicate that new frontiers of hydrocarbon exploration in the west African basins must consider the Tertiary reservoirs in the offshore area of Niger Delta, the reservoirs of the rift sequences in the shallow-water areas of south Gabon, Congo, and Cuanza basins, and the reservoirs from the drift sequences (post-salt) in the deep-water areas of Gabon, Congo Cabinda, and Cuanza basins.« less

Passive optical remote sensing of Congo River bathymetry using Landsat

NASA Astrophysics Data System (ADS)

Ache Rocha Lopes, V.; Trigg, M. A.; O'Loughlin, F.; Laraque, A.

2014-12-01

While there have been notable advances in deriving river characteristics such as width, using satellite remote sensing datasets, deriving river bathymetry remains a significant challenge. Bathymetry is fundamental to hydrodynamic modelling of river systems and being able to estimate this parameter remotely would be of great benefit, especially when attempting to model hard to access areas where the collection of field data is difficult. One such region is the Congo Basin, where due to past political instability and large scale there are few studies that characterise river bathymetry. In this study we test whether it is possible to use passive optical remote sensing to estimate the depth of the Congo River using Landsat 8 imagery in the region around Malebo Pool, located just upstream of the Kinshasa gauging station. Methods of estimating bathymetry using remotely sensed datasets have been used extensively for coastal regions and now more recently have been demonstrated as feasible for optically shallow rivers. Previous river bathymetry studies have focused on shallow rivers and have generally used aerial imagery with a finer spatial resolution than Landsat. While the Congo River has relatively low suspended sediment concentration values the application of passive bathymetry estimation to a river of this scale has not been attempted before. Three different analysis methods are tested in this study: 1) a single band algorithm; 2) a log ratio method; and 3) a linear transform method. All three methods require depth data for calibration and in this study area bathymetry measurements are available for three cross-sections resulting in approximately 300 in-situ measurements of depth, which are used in the calibration and validation. The performance of each method is assessed, allowing the feasibility of passive depth measurement in the Congo River to be determined. Considering the scarcity of in-situ bathymetry measurements on the Congo River, even an approximate estimate of depths from these methods will be of considerable value in its hydraulic characterisation.

Water Storage Changes using Floodplain Bathymetry from InSAR and satellite altimetry in the Congo River Basin

NASA Astrophysics Data System (ADS)

Yuan, T.; Lee, H.; Jung, H. C.; Beighley, E.; Alsdorf, D. E.

2016-12-01

Extensive wetlands and swamps expand along the Congo River and its tributaries. These wetlands store water and attenuate flood wave during high water season. Substantial dissolved and solid substances are also transported with the water flux, influencing geochemical environment and biogeochemistry processes both in the wetlands and the river. To understand the role of the wetlands in partitioning the surface water and the accompanied material movement, water storage change is one of the most fundamental observations. The water flow through the wetlands is complex, affected by topography, vegetation resistance, and hydraulic variations. Interferometric Synthetic Aperture Radar (InSAR) has been successfully used to map relative water level changes in the vegetated wetlands with high spatial resolution. By examining interferograms generated from ALOS PALSAR along the middle reach of the Congo River floodplain, we found greater water level changes near the Congo mainstem. Integrated analysis of InSAR and Envisat altimetry data has shown that proximal floodplain with higher water level change has lower elevation during dry season. This indicates that the spatial variation of water level change in the Congo floodplain is mostly controlled by floodplain bathymetry. A method based on water level and bathymetry model is proposed to estimate water storage change. The bathymetry model is composed of (1) elevation at the intersection of the floodplain and the river and (2) floodplain bathymetry slope. We first constructed the floodplain bathymetry by selecting an Envisat altimetry profile during low water season to estimate elevation at the intersection of the floodplain and the river. Floodplain bathymetry slope was estimated using InSAR measurements. It is expected that our new method can estimate water storage change with higher temporal resolution corresponding to altimeter's repeat cycle. In addition, given the multi-decadal archive of satellite altimetry measurements, our method suggests a way to estimate interannual water storage change over a long time span in Congo.

Incorporating DNA barcodes into a multi-year inventory of the fishes of the hyperdiverse Lower Congo River, with a multi-gene performance assessment of the genus Labeo as a case study.

PubMed

Lowenstein, Jacob H; Osmundson, Todd W; Becker, Sven; Hanner, Robert; Stiassny, Melanie L J

2011-10-01

Here we describe preliminary efforts to integrate DNA barcoding into an ongoing inventory of the Lower Congo River (LCR) ichthyofauna. The 350 km stretch of the LCR from Pool Malebo to Boma includes the world's largest river rapids. The LCR ichthyofauna is hyperdiverse and rich in endemism due to high habitat heterogeneity, numerous dispersal barriers, and its downstream location in the basin. We have documented 328 species from the LCR, 25% of which are thought to be endemic. In addition to detailing progress made to generate a reference sequence library of DNA barcodes for these fishes, we ask how DNA can be used at the current stage of the Fish Barcode of Life initiative, as a work in progress currently of limited utility to a wide audience. Two possibilities that we explore are the potential for DNA barcodes to generate discrete diagnostic characters for species, and to help resolve problematic taxa lacking clear morphologically diagnostic characters such as many species of the cyprinid genus Labeo, which we use as a case study. Our molecular analysis helped to clarify the validity of some species that were the subject of historical debate, and we were able to construct a molecular key for all monophyletic and morphologically recognizable species. Several species sampled from across the Congo Basin and widely distributed throughout Central and West Africa were recovered as paraphyletic based on our molecular data. Our study underscores the importance of generating reference barcodes for specimens collected from, or in close proximity to, type localities, particularly where species are poorly understood taxonomically and the extent of their geographical distributions have yet to be established.

Prevalence of antibodies to alphaviruses and flaviviruses in free-ranging game animals and nonhuman primates in the greater Congo basin.

PubMed

Kading, Rebekah C; Borland, Erin M; Cranfield, Mike; Powers, Ann M

2013-07-01

Vector-borne and zoonotic pathogens have comprised a significant proportion of the emerging infectious diseases in humans in recent decades. The role of many wildlife species as reservoirs for arthropod-borne viral pathogens is poorly understood. We investigated the exposure history of various African wildlife species from the Congo basin to mosquito-borne flaviviruses and alphaviruses by testing archived serum samples. Sera from 24 African forest buffalo (Syncerus caffer nanus), 34 African elephants (Loxodonta africana), 40 duikers (Cephalophus and Philantomba spp.), 25 mandrills (Mandrillus sphinx), 32 mountain gorillas (Gorilla beringei beringei), five Grauer's gorillas (Gorilla beringei graueri), two L'Hoest's monkeys (Cercopithecus lhoesti), two golden monkeys (Cercopithecus kandti), and three chimpanzees (Pan troglodytes) sampled between 1991 and 2009 were tested for antibodies against chikungunya virus (CHIKV), o'nyong-nyong virus (ONNV), West Nile virus (WNV), dengue 2 virus (DENV-2), and yellow fever virus (YFV) by plaque reduction neutralization test. Specific neutralizing antibodies against ONNV were found in African forest buffalo in the Democratic Republic of the Congo (DRC) and Gabon, duikers in the DRC, and mandrills in Gabon, providing novel evidence of enzootic circulation of ONNV in these countries. African forest buffalo in the DRC and Gabon also demonstrated evidence of exposure to CHIKV, WNV, and DENV-2, while mandrills in Gabon were antibody positive for CHIKV, DENV-2, WNV, and YFV. All of the elephants tested had a strong neutralizing antibody response to WNV. We also document results from a survey of gorillas for arboviruses, of which 4/32 (13%) had antibody to an alphavirus or flavivirus. Overall, our results demonstrate a high prevalence of neutralizing antibodies against multiple arboviruses in wildlife in equatorial Africa.

Radar Mosaic of Africa

NASA Image and Video Library

1999-09-23

This is an image of equatorial Africa, centered on the equator at longitude 15degrees east. This image is a mosaic of almost 4,000 separate images obtained in 1996 by the L-band imaging radar onboard the Japanese Earth Resources Satellite. Using radar to penetrate the persistent clouds prevalent in tropical forests, the Japanese Earth Resources Satellite was able for the first time to image at high resolution this continental scale region during single flooding seasons. The area shown covers about 7.4 million square kilometers (2.8 million square miles) of land surface, spans more than 5,000 kilometers(3,100 miles) east and west and some 2,000 kilometers (1,240 miles) north and south. North is up in this image. At the full resolution of the mosaic (100 meters or 330 feet), this image is more than 500 megabytes in size, and was processed from imagery totaling more than 60 gigabytes. Central Africa was imaged twice in 1996, once between January and March, which is the major low-flood season in the Congo Basin, and once between October and November, which is the major high-flood season in the Congo Basin. The red color corresponds to the data from the low-flood season, the green to the high-flood season, and the blue to the "texture" of the low-flood data. The forests appear green as a result, the flooded and palm forests, as well as urban areas, appear yellow, the ocean and lakes appear black, and savanna areas appear blue, black or green, depending on the savanna type, surface topography and other factors. The areas of the image that are black and white were mapped only between January and March 1996. In these areas, the black areas are savanna or open water, the gray are forests, and the white areas are flooded forests or urban areas. The Congo River dominates the middle of the image, where the nearby forests that are periodically flooded by the Congo and its tributaries stand out as yellow. The Nile River flows north from Lake Victoria in the middle right of the color portion of the mosaic. http://photojournal.jpl.nasa.gov/catalog/PIA01348

DOE Office of Scientific and Technical Information (OSTI.GOV)

McGinnis, J.P.; Karner, G.D.; Driscoll, N.W.

The tectonic and stratigraphic development of the Congo continental margin reflects the timing, magnitude, and distribution of lithospheric extension responsible for its formation. Details of the lithospheric extension process are recorded in the stratigraphic successions preserved along and across the margin. By using the stratal relationships (e.g., onlap, downlap, and truncation) and lithofacies determined from seismic reflection and exploratory well data as input into our basin-modeling strategy, we have developed an integrated approach to determine the relationship between the timing, magnitude, and distribution of lithospheric extension across the margin. Two hinge zones, an eastern and Atlantic hinge formed along themore » Congo margin in response to discrete extensional events occurring from the Berriasian to the Aptian. The eastern hinge zone demarcates the eastern limit of the broadly distributed Berriasian extension. This extension resulted in the formation of deep anoxic, lacustrine systems. In contrast, the Atlantic hinge, located [approximately]90 km west of the eastern hinge, marks the eastern limit of a second phase of extension, which began in the Hauterivian. Consequent footwall uplift and rotation exposed the earlier synrift and prerift stratigraphy to at least wave base causing varying amounts of erosional truncation across the Atlantic hinge zone along much of the Gabon, Congo, and Angola margins. The absence of the Melania Formation across the Congo margin implies that uplift of the Atlantic hinge was relatively minor compared to that across the Angola and Gabon margins. In addition, material eroded from the adjacent and topographically higher hinge zones may in part account for the thick wedge of sediment deposited seaward of the Congo Atlantic hinge. A third phase of extension reactivated both the eastern and Atlantic hinge zones and was responsible for creating the accommodation space for Marnes Noires source rock deposition.« less

Assessment of the Protective Effect of Imvamune and Acam2000 Vaccines against Aerosolized Monkeypox Virus in Cynomolgus Macaques

PubMed Central

Graham, Victoria A.; Bewley, Kevin R.; Dennis, Mike; Taylor, Irene; Funnell, Simon G. P.; Bate, Simon R.; Steeds, Kimberley; Tipton, Thomas; Bean, Thomas; Hudson, Laura; Atkinson, Deborah J.; McLuckie, Gemma; Charlwood, Melanie; Roberts, Allen D. G.; Vipond, Julia

2013-01-01

To support the licensure of a new and safer vaccine to protect people against smallpox, a monkeypox model of infection in cynomolgus macaques, which simulates smallpox in humans, was used to evaluate two vaccines, Acam2000 and Imvamune, for protection against disease. Animals vaccinated with a single immunization of Imvamune were not protected completely from severe and/or lethal infection, whereas those receiving either a prime and boost of Imvamune or a single immunization with Acam2000 were protected completely. Additional parameters, including clinical observations, radiographs, viral load in blood, throat swabs, and selected tissues, vaccinia virus-specific antibody responses, immunophenotyping, extracellular cytokine levels, and histopathology were assessed. There was no significant difference (P > 0.05) between the levels of neutralizing antibody in animals vaccinated with a single immunization of Acam2000 (132 U/ml) and the prime-boost Imvamune regime (69 U/ml) prior to challenge with monkeypox virus. After challenge, there was evidence of viral excretion from the throats of 2 of 6 animals in the prime-boost Imvamune group, whereas there was no confirmation of excreted live virus in the Acam2000 group. This evaluation of different human smallpox vaccines in cynomolgus macaques helps to provide information about optimal vaccine strategies in the absence of human challenge studies. PMID:23658452

An initial investigation into the organic matter biogeochemistry of the Congo River

NASA Astrophysics Data System (ADS)

Spencer, Robert G. M.; Hernes, Peter J.; Aufdenkampe, Anthony K.; Baker, Andy; Gulliver, Pauline; Stubbins, Aron; Aiken, George R.; Dyda, Rachael Y.; Butler, Kenna D.; Mwamba, Vincent L.; Mangangu, Arthur M.; Wabakanghanzi, Jose N.; Six, Johan

2012-05-01

The Congo River, which drains pristine tropical forest and savannah and is the second largest exporter of terrestrial carbon to the ocean, was sampled in early 2008 to investigate organic matter (OM) dynamics in this historically understudied river basin. We examined the elemental (%OC, %N, C:N), isotopic (δ13C, Δ14C, δ15N) and biochemical composition (lignin phenols) of coarse particulate (>63 μm; CPOM) and fine particulate (0.7-63 μm; FPOM) OM and DOC, δ13C, Δ14C and lignin phenol composition with respect to dissolved OM (<0.7 μm; DOM) from five sites in the Congo River Basin. At all sample locations the organic carbon load was dominated by the dissolved phase (˜82-89% of total organic carbon) and the total suspended sediment load was principally fine particulate material (˜81-91% fine suspended sediment). Distinct compositional and isotopic differences were observed between all fractions. Congo CPOM, FPOM and DOM all originated from vegetation and soil inputs as evidenced by elemental, isotopic and lignin phenol data, however FPOM was derived from much older carbon pools (mean Δ14C = -62.2 ± -13.2‰, n = 5) compared to CPOM and DOM (mean Δ14C = 55.7 ± 30.6‰, n = 4 and 73.4 ± 16.1‰, n = 5 respectively). The modern radiocarbon ages for DOM belie a degraded lignin compositional signature (i.e. elevated acid:aldehyde ratios (Ad:Al) relative to CPOM and FPOM), and indicate that the application of OM degradation patterns derived from particulate phase studies to dissolved samples needs to be reassessed: these elevated ratios are likely attributable to fractionation processes during solubilization of plant material. The relatively low DOM carbon-normalized lignin yields (Λ8; 0.67-1.12 (mg(100 mg OC)-1)) could also reflect fractionation processes, however, they have also been interpreted as an indication of significant microbial or algal sources of DOM. CPOM appears to be well preserved higher vascular plant material as evidenced by its modern radiocarbon age, elevated C:N (17.2-27.1) and Λ8 values (4.56-7.59 (mg(100 mg OC)-1)). In relation to CPOM, the aged FPOM fraction (320-580 ybp 14C ages) was comparatively degraded, as demonstrated by its nitrogen enrichment (C:N 11.4-14.3), lower Λ8 (2.80-4.31 (mg(100 mg OC)-1)) and elevated lignin Ad:Al values similar to soil derived OM. In this study we observed little modification of the OM signature from sample sites near the cities of Brazzaville and Kinshasa to the head of the estuary (˜350 km) highlighting the potential for future studies to assess seasonal and long-term OM dynamics from this logistically feasible location and derive relevant information with respect to OM exported to the Atlantic Ocean. The relative lack of OM data for the Congo River Basin highlights the importance of studies such as this for establishing baselines upon which to gauge future change.

Global Physiographic and Climatic Maps to Support Revision of Environmental Testing Guidelines

DTIC Science & Technology

2009-07-06

precipitation and boarded by High and Low Relief Mountains or Interior Plains and Plateaus, such as the Amazon River Basin in South America and the Congo...taxonomy system. These form in hot climates with continual moisture availability, typically thought to occur only beneath tropical rainforests , though...Montane Tropical Forest Tropical Degraded Forest Seasonal Tropical Forest Rain Green Tropical Forest Tropical Rainforest FIGURE 6-1 DATE: 6-02

Implications of changes in tropical shifting cultivation intensification on land productivity and GHG-related biogeochemistry

NASA Astrophysics Data System (ADS)

Bustier, Bernard; Ngoy, Alfred; Pietsch, Stephan; Mosnier, Aline

2017-04-01

Traditional shifting cultivation used to be a sustainable type of land use for the subsistence of populations in tropical rainforests. The vast resource of moist tropical forests together with low population densities allowed for long fallow periods on sparsely distributed slash and burn parcels with large areas of untouched forest in between. Population growth and concomitant increase in land demand for subsistence as well as increasing infrastructure development for commercial forestry, cash crops and mining, however, altered the picture over recent decades. As a result, fallow periods were reduced due to lack of pristine land. In this study we use field data and modeling results from the Congo Basin to assess the impacts of reduced fallow periods on Carbon sequestration dynamics using a BGC model calibrated and validated with > 150 research plots distributed over the western Congo Basin and representing different management and land use histories. We find that the average carbon sequestration rate reduces over the number of cultivation cycles and that a reduction of the fallow from 10 years to 7 years reduce the average carbon sequestration between 13 and 21% and from 7 years to 4 years between 23 and 29% depending on soil fertility. Results will be discussed in the context of population growth and changes in environmetal conditions.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ahmed, Mohamed; Sultan, Mohamed; Wahr, John

Inter-annual trends in terrestrial water storage (TWS) were extracted from monthly (01/2003–09/2012) Gravity Recovery and Climate Experiment (GRACE) data acquired over Africa and correlated (in a geographic information system [GIS] environment) with relevant temporal remote sensing, geologic, and hydrologic datasets. Findings include the following: (1) large sectors of Africa are undergoing statistically significant TWS variations (+ 44 mm/yr to -15 mm/yr) due to natural and anthropogenic causes; (2) warming of the tropical Atlantic Ocean apparently intensified Atlantic monsoons and increased precipitation and TWS over western and central Africa; (3) warming in the central Indian Ocean decreased precipitation and TWS overmore » eastern Africa; (4) the high frequency of flooding events increased TWS over the Zambezi and Okavango basins; (5) extraction of fossil groundwater decreased TWS over the Saharan aquifers; (6) deforestation decreased TWS in three subbasins (Ubangi, Congo, and Sangha) of the Congo River Basin; and (7) the construction of dams increased TWS in the Blue Nile and Atbara subbasins. Given the 10 years of monthly GRACE data acquired on the subbasin scale across the globe, as well as the plans underway for deployment of a GRACE-FO and GRACE-II, using GRACE-derived TWS data should be considered an alternative, viable index for measuring temporal and spatial variations in water availability.« less

Mapping Land Cover and Land Use Changes in the Congo Basin Forests with Optical Satellite Remote Sensing: a Pilot Project Exploring Methodologies that Improve Spatial Resolution and Map Accuracy

NASA Astrophysics Data System (ADS)

Molinario, G.; Baraldi, A.; Altstatt, A. L.; Nackoney, J.

2011-12-01

The University of Maryland has been a USAID Central Africa Rregional Program for the Environment (CARPE) cross-cutting partner for many years, providing remote sensing derived information on forest cover and forest cover changes in support of CARPE's objectives of diminishing forest degradation, loss and biodiversity loss as a result of poor or inexistent land use planning strategies. Together with South Dakota State University, Congo Basin-wide maps have been provided that map forest cover loss at a maximum of 60m resolution, using Landsat imagery and higher resolution imagery for algorithm training and validation. However, to better meet the needs within the CARPE Landscapes, which call for higher resolution, more accurate land cover change maps, UMD has been exploring the use of the SIAM automatic spectral -rule classifier together with pan-sharpened Landsat data (15m resolution) and Very High Resolution imagery from various sources. The pilot project is being developed in collaboration with the African Wildlife Foundation in the Maringa Lopori Wamba CARPE Landscape. If successful in the future this methodology will make the creation of high resolution change maps faster and easier, making it accessible to other entities in the Congo Basin that need accurate land cover and land use change maps in order, for example, to create sustainable land use plans, conserve biodiversity and resources and prepare Reducing Emissions from forest Degradation and Deforestation (REDD) Measurement, Reporting and Verification (MRV) projects. The paper describes the need for higher resolution land cover change maps that focus on forest change dynamics such as the cycling between primary forests, secondary forest, agriculture and other expanding and intensifying land uses in the Maringa Lopori Wamba CARPE Landscape in the Equateur Province of the Democratic Republic of Congo. The Methodology uses the SIAM remote sensing imagery automatic spectral rule classifier, together with pan-sharpened Landsat imagery with 15m resolution and Very High Resolution imagery from different sensors, obtained from the Department of Defense database that was recently opened to NASA and its Earth Observation partners. Particular emphasis is placed on the detection of agricultural fields and their expansion in primary forests or intensification in secondary forests and fallow fields, as this is the primary driver of deforestation in this area. Fields in this area area also of very small size and irregular shapes, often partly obscured by neighboring forest canopy, hence the technical challenge of correctly detecting them and tracking them through time. Finally, the potential for use of this methodology in other regions where information on land cover changes is needed for land use sustainability planning, is also addressed.

Species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time PCR assay.

PubMed

Maksyutov, Rinat A; Gavrilova, Elena V; Shchelkunov, Sergei N

2016-10-01

A method of one-stage rapid detection and differentiation of epidemiologically important variola virus (VARV), monkeypox virus (MPXV), and varicella-zoster virus (VZV) utilizing multiplex real-time TaqMan PCR assay was developed. Four hybridization probes with various fluorescent dyes and the corresponding fluorescence quenchers were simultaneously used for the assay. The hybridization probes specific for the VARV sequence contained FAM/BHQ1 as a dye/quencher pair; MPXV-specific, JOE/BHQ1; VZV-specific, TAMRA/BHQ2; and internal control-specific, Cy5/BHQ3. The specificity and sensitivity of the developed method were assessed by analyzing DNA of 32 strains belonging to orthopoxvirus and herpesvirus species. Copyright © 2016 Elsevier B.V. All rights reserved.

Basin-Scale Freshwater Storage Trends from GRACE

NASA Astrophysics Data System (ADS)

Famiglietti, J.; Kiel, B.; Frappart, F.; Syed, T. H.; Rodell, M.

2006-12-01

Four years have passed since the GRACE satellite tandem began recording variations in Earth's gravitational field. On monthly to annual timescales, variations in the gravity signal for a given location correspond primarily to changes in water storage. GRACE thus reveals, in a comprehensive, vertically-integrated manner, which areas and basins have experienced net increases or decreases in water storage. GRACE data (April 2002 to November 2005) released by the Center for Space Research at the University of Texas at Austin (RL01) is used for this study. Model-based data from GLDAS (Global Land Data Assimilation System) is integrated into this study for comparison with the CSR GRACE data. Basin-scale GLDAS storage trends are similar to those from GRACE, except in the Arctic, likely due to the GLDAS snow module. Outside of the Arctic, correlation of GRACE and GLDAS data confirms significant basin-scale storage trends across the GRACE data collection period. Sharp storage decreases are noted in the Congo, Zambezi, Mekong, Parana, and Yukon basins, among others. Significant increases are noted in the Niger, Lena, and Volga basins, and others. Current and future work involves assessment of these trends and their causes in the context of hydroclimatological variability.

About Monkeypox

MedlinePlus

... Contagiosum Orf Virus (Sore Mouth Infection) Poxvirus and Rabies Branch Travelers’ Health: Smallpox & Other Orthopoxvirus-Associated Infections ... Contagiosum Orf Virus (Sore Mouth Infection) Poxvirus and Rabies Branch Travelers’ Health: Smallpox & Other Orthopoxvirus-Associated Infections ...

Comparative Pathology of Smallpox and Monkeypox in Man and Macaques

PubMed Central

Cann, J. A.; Jahrling, P. B.; Hensley, L. E.; Wahl-Jensen, V.

2012-01-01

Summary In the three decades since the eradication of smallpox and cessation of routine vaccination, the collective memory of the devastating epidemics caused by this orthopoxvirus has waned, and the human population has become increasingly susceptible to a disease that remains high on the list of possible bioterrorism agents. Research using surrogate orthopoxviruses in their natural hosts, as well as limited variola virus research in animal models, continues worldwide; however, interpretation of findings is often limited by our relative lack of knowledge about the naturally occurring disease. For modern comparative pathologists, many of whom have no first-hand knowledge of naturally occurring smallpox, this work provides a contemporary review of this historical disease, as well as discussion of how it compares with human monkeypox and the corresponding diseases in macaques. PMID:22884034

Hydrologic control of carbon cycling and aged carbon discharge in the Congo River basin

NASA Astrophysics Data System (ADS)

Schefuß, Enno; Eglinton, Timothy I.; Spencer-Jones, Charlotte L.; Rullkötter, Jürgen; de Pol-Holz, Ricardo; Talbot, Helen M.; Grootes, Pieter M.; Schneider, Ralph R.

2016-09-01

The age of organic material discharged by rivers provides information about its sources and carbon cycling processes within watersheds. Although elevated ages in fluvially transported organic matter are usually explained by erosion of soils and sedimentary deposits, it is commonly assumed that mainly young organic material is discharged from flat tropical watersheds due to their extensive plant cover and rapid carbon turnover. Here we present compound-specific radiocarbon data of terrigenous organic fractions from a sedimentary archive offshore the Congo River, in conjunction with molecular markers for methane-producing land cover reflecting wetland extent. We find that the Congo River has been discharging aged organic matter for several thousand years, with apparently increasing ages from the mid- to the Late Holocene. This suggests that aged organic matter in modern samples is concealed by radiocarbon from atmospheric nuclear weapons testing. By comparison to indicators for past rainfall changes we detect a systematic control of organic matter sequestration and release by continental hydrology, mediating temporary carbon storage in wetlands. As aridification also leads to exposure and rapid remineralization of large amounts of previously stored labile organic matter, we infer that this process may cause a profound direct climate feedback that is at present underestimated in carbon cycle assessments.

Mapping Water Level Dynamics over Central Congo River Using PALSAR Images, Envisat Altimetry, and Landsat NDVI Data

NASA Astrophysics Data System (ADS)

Kim, D.; Lee, H.; Jung, H. C.; Beighley, E.; Laraque, A.; Tshimanga, R.; Alsdorf, D. E.

2016-12-01

Rivers and wetlands are very important for ecological habitats, and it plays a key role in providing a source of greenhouse gases (CO2 and CH4). The floodplains ecosystems depend on the process between the vegetation and flood characteristics. The water level is a prerequisite to an understanding of terrestrial water storage and discharge. Despite the lack of in situ data over the Congo Basin, which is the world's third largest in size ( 3.7 million km2), and second only to the Amazon River in discharge ( 40,500 m3 s-1 annual average between 1902 and 2015 in the main Brazzaville-Kinshasa gauging station), the surface water level dynamics in the wetlands have been successfully estimated using satellite altimetry, backscattering coefficients (σ0) from Synthetic Aperture Radar (SAR) images and, interferometric SAR technique. However, the water level estimation of the Congo River remains poorly quantified due to the sparse orbital spacing of radar altimeters. Hence, we essentially have limited information only over the sparsely distributed the so-called "virtual stations". The backscattering coefficients from SAR images have been successfully used to distinguish different vegetation types, to monitor flood conditions, and to access soil moistures over the wetlands. However, σ0 has not been used to measure the water level changes over the open river because of very week return signal due to specular scattering. In this study, we have discovered that changes in σ0 over the Congo River occur mainly due to the water level changes in the river with the existence of the water plants (macrophytes, emergent plants, and submersed plant), depending on the rising and falling stage inside the depression of the "Cuvette Centrale". We expand the finding into generating the multi-temporal water level maps over the Congo River using PALSAR σ0, Envisat altimetry, and Landsat Normalized Difference Vegetation Index (NDVI) data. We also present preliminary estimates of the river discharge using the water level maps.

Modeling sediment supply of the Congo watershed since the last 23 ka.

NASA Astrophysics Data System (ADS)

Molliex, Stéphane; Kettner, Albert J.; Laurent, Dimitri; Droz, Laurence; Marsset, Tania; Laraque, Alain; Rabineau, Marina

2017-04-01

The Congo River is the world's second river in term of drainage area (3.7 millions of km2) and water discharge (42,000 m3.s-1). Located in equatorial Africa, the basin extends over the two hemispheres, leading to an annual homogeneous repartition of climatic parameters and modest variation in intra-annual discharge. Monitored for decades, a large dataset is available for both the hydrology and sediment load for the Congo system. Moreover, the Quaternary Congo turbidite system geometry has been widely studied and an abundance of paleo-environmental parameters have been inferred from chemical proxies analyzed from offshore cores. These numerous data, both onshore and offshore, allow for accurate calibration of numeric modeling and for efficient comparison between observed and simulated data. This study aims (i) to quantify the evolution of sediment supply leaving the Congo watershed during the last 23 ka; (ii) to decipher the forcing parameters controlling the sediment supply over glacial/interglacial stages. HydroTrend is a model that simulates water discharge and sediment load leaving a hydrologic system. It is based on morphologic, climatic, hydrologic, lithologic, land cover and anthropogenic factors. After calibrating the present-day discharge and sediment load, we simulated discharge and sediment supply over 23 ka, integrating the changes in environmental conditions during this period. Results show that present-day simulations fit the observed data well if a significant part of sediments is being trapped by the catchment, in the floodplain. The long-term simulations show that the changes in climatic conditions (temperature and precipitations) between glacial and interglacial stages only account for a maximum variation of about 20 % of the sediment supply. The resulting land cover changes are most likely a more significant factor controlling the sediment supply; the loss of forest during colder and dryer stages can be responsible for up to 50 % of sediment supply increase.

Lightning climatology in the Congo Basin

NASA Astrophysics Data System (ADS)

Soula, S.; Kasereka, J. Kigotsi; Georgis, J. F.; Barthe, C.

2016-09-01

The lightning climatology of the Congo Basin including several countries of Central Africa is analysed in detail for the first time. It is based on data from the World Wide Lightning Location Network (WWLLN), for the period from 2005 to 2013. A comparison of these data with Lightning Imaging Sensor (LIS) data for the same period shows the relative detection efficiency of the WWLLN (DE) in the 2500 km × 2500 km region increases from about 1.70% in the beginning of the period to 5.90% in 2013, and it is in agreement with previous results for other regions of the world. However, the increase of DE is not uniform over the whole region. The average monthly flash rate describes an annual cycle with a strong activity from October to March and a low one from June to August, associated with the ITCZ migration but not exactly symmetrical on both sides of the equator. The zonal distribution of the lightning flashes exhibits a maximum between 1°S and 2°S and about 56% of the flashes are located south of the equator in the 10°S-10°N interval. The diurnal evolution of the flash rate has a maximum between 1400 and 1700 UTC, according to the reference year. The annual flash density and number of stormy days show a sharp maximum localized in the eastern part of Democratic Republic of Congo (DRC) regardless of the reference year and the period of the year. These maxima reach 12.86 fl km- 2 and 189 days, respectively, in 2013, and correspond to a very active region located at the rear of the Virunga mountain range at altitudes that exceed 3000 m. The presence of these mountains plays a role in the thunderstorm development along the year. The estimation of this local maximum of the lightning density by taking into account the DE, leads to a value consistent with that of the global climatology by Christian et al. (2003).

Lightning climatology in the Congo Basin: detailed analysis

NASA Astrophysics Data System (ADS)

Soula, Serge; Kigotsi, Jean; Georgis, Jean-François; Barthe, Christelle

2016-04-01

The lightning climatology of the Congo Basin including several countries of Central Africa is analyzed in detail for the first time. It is based on World Wide Lightning Location Network (WWLLN) data for the period from 2005 to 2013. A comparison of these data with the Lightning Imaging Sensor (LIS) data for the same period shows the WWLLN detection efficiency (DE) in the region increases from about 1.70 % in the beginning of the period to 5.90 % in 2013, relative to LIS data, but not uniformly over the whole 2750 km × 2750 km area. Both the annual flash density and the number of stormy days show sharp maximum values localized in eastern of Democratic Republic of Congo (DRC) and west of Kivu Lake, regardless of the reference year and the period of the year. These maxima reach 12.86 fl km-2 and 189 days, respectively, in 2013, and correspond with a very active region located at the rear of the Virunga mountain range characterised with summits that can reach 3000 m. The presence of this range plays a role in the thunderstorm development along the year. The estimation of this local maximum of the lightning density by taking into account the DE, leads to a value consistent with that of the global climatology by Christian et al. (2003) and other authors. Thus, a mean maximum value of about 157 fl km-2 y-1 is found for the annual lightning density. The zonal distribution of the lightning flashes exhibits a maximum between 1°S and 2°S and about 56 % of the flashes located below the equator in the 10°S - 10°N interval. The diurnal evolution of the flash rate has a maximum between 1400 and 1700 UTC, according to the reference year, in agreement with previous works in other regions of the world.

A modelling approach to estimate carbon emissions from D.R.C. deforestation

NASA Astrophysics Data System (ADS)

Najdovski, Nicolas; Poulter, Benjamin; Defourny, Pierre; Moreau, Inès; Maignan, Fabienne; Ciais, Philippe; Verhegghen, Astrid; Kibambe Lubamba, Jean-Paul; Jungers, Quentin; De Weirdt, Marjolein; Verbeeck, Hans; MacBean, Natasha; Peylin, Philippe

2014-05-01

With its 1.8 million squared kilometres, the Congo basin dense forest represents the second largest contiguous forest of the world. These extensive forest ecosystems play a significant role in the regulation of global climate by their potential carbon dioxide emissions and carbon storage. Under a stable climate, the vegetation, assumed to be at the equilibrium, is known to present neutral emissions over a year with seasonal variations. However, modifications in temperatures, precipitations, CO2 atmospheric concentrations have the potential to modify this balance leading to higher or lower biomass storage. In addition, deforestation and forest degradation have played a significant role over the past several decades and are expected to become increasingly important in the future. Here, we quantify the relative effects of deforestation and 21st century climate change on carbon emissions in Congo Basin over the next three decades (2005-2035). Carbon dioxide emissions are estimated using a series of moderate resolution (10 km) vegetation maps merged with spatially explicit deforestation projections and developed to work with a prognostic carbon cycle model. The inversion of the deforestation model allowed hindcast land-use patterns back to 1800 by using land cover change rates based on the HYDE database. Simulations were made over the Democratic Republic of Congo (DRC) using the ORCHIDEE dynamic global vegetation model with climate forcing from the CMIP5 Representative Concentration Pathway 8.5 scenario for the HadGEM2. Two simulations were made, a reference simulation with land cover fixed at 2005 and a land cover change simulation with changing climate and CO2, to quantify the net land cover change emissions and climate emissions directly. Because of the relatively high resolution of the model simulations, the spatial patterns of human-driven carbon losses can be tracked in the context of climate change, providing information for mitigation and vulnerability activities.

Isotopic composition of waters from Ethiopia and Kenya: Insights into moisture sources for eastern Africa

NASA Astrophysics Data System (ADS)

Levin, Naomi E.; Zipser, Edward J.; Cerling, Thure E.

2009-12-01

Oxygen and deuterium isotopic values of meteoric waters from Ethiopia are unusually high when compared to waters from other high-elevation settings in Africa and worldwide. These high values are well documented; however, the climatic processes responsible for the isotopic anomalies in Ethiopian waters have not been thoroughly investigated. We use isotopic data from waters and remote data products to demonstrate how different moisture sources affect the distribution of stable isotopes in waters from eastern Africa. Oxygen and deuterium stable isotopic data from 349 surface and near-surface groundwaters indicate isotopic distinctions between waters in Ethiopia and Kenya and confirm the anomalous nature of Ethiopian waters. Remote data products from the Tropical Rainfall Measuring Mission (TRMM) and National Centers for Environmental Prediction (NCEP) reanalysis project show strong westerly and southwesterly components to low-level winds during precipitation events in western and central Ethiopia. This is in contrast to the easterly and southeasterly winds that bring rainfall to Kenya and southeastern Ethiopia. Large regions of high equivalent potential temperatures (θe) at low levels over the Sudd and the Congo Basin demonstrate the potential for these areas as sources of moisture and convective instability. The combination of wind direction data from Ethiopia and θe distribution in Africa indicates that transpired moisture from the Sudd and the Congo Basin is likely responsible for the high isotopic values of rainfall in Ethiopia.

42 CFR 73.3 - HHS select agents and toxins.

Code of Federal Regulations, 2012 CFR

2012-10-01

... virus Monkeypox virus Reconstructed replication competent forms of the 1918 pandemic influenza virus containing any portion of the coding regions of all eight gene segments (Reconstructed 1918 Influenza virus...

Using the Ground Squirrel (Marmota bobak) as an Animal Model to Assess Monkeypox Drug Efficacy.

PubMed

Sergeev, A A; Kabanov, A S; Bulychev, L E; Sergeev, A A; Pyankov, O V; Bodnev, S A; Galahova, D O; Zamedyanskaya, A S; Titova, K A; Glotova, T I; Taranov, O S; Omigov, V V; Shishkina, L N; Agafonov, A P; Sergeev, A N

2017-02-01

In experiments to study the sensitivity of ground squirrels (Marmota bobak) to monkeypox virus (MPXV) at intranasal challenge, expressed pox-like clinical symptoms (hyperthermia, lymphadenitis, skin rash all over the body and mucous membranes and others) were observed 7-9 days post-infection. The 50% infective dose (ID 50 ) of MPXV for these marmots determined by the presence of clinical signs of the disease was 2.2 log 10 PFU. Some diseased marmots (about 40%) died 13-22 days post-infection, and the mortality rate was weakly dependent on MPXV infective dose. Lungs with trachea were primary target organs of marmots challenged intranasally (with ~30 ID 50 ). The pathogen got to secondary target organs of the animals mainly via the lymphatic way (with replication in bifurcation lymph nodes). Lungs with trachea, nasal mucosa and skin were the organs where the maximum MPXV amounts accumulated in these animals. Evidences of the pathogen presence and replication were revealed in these and subcutaneously infected marmots in the traditional primary target cells for MPXV (macrophages and respiratory tract epitheliocytes), as well as in some other cells (endotheliocytes, plasmocytes, fibroblasts, reticular and smooth muscle cells). Our use of this animal species to assess the antiviral efficacy of some drugs demonstrated the agreement of the obtained results with those described in scientific literature, which opens up the prospects of using marmots as animal models for monkeypox to develop therapeutic and preventive anti-smallpox drugs. © 2015 Blackwell Verlag GmbH.

The highly virulent variola and monkeypox viruses express secreted inhibitors of type I interferon

PubMed Central

Fernández de Marco, María del Mar; Alejo, Alí; Hudson, Paul; Damon, Inger K.; Alcami, Antonio

2010-01-01

Variola virus (VARV) caused smallpox, one of the most devastating human diseases and the first to be eradicated, but its deliberate release represents a dangerous threat. Virulent orthopoxviruses infecting humans, such as monkeypox virus (MPXV), could fill the niche left by smallpox eradication and the cessation of vaccination. However, immunomodulatory activities and virulence determinants of VARV and MPXV remain largely unexplored. We report the molecular characterization of the VARV- and MPXV-secreted type I interferon-binding proteins, which interact with the cell surface after secretion and prevent type I interferon responses. The proteins expressed in the baculovirus system have been purified, and their interferon-binding properties characterized by surface plasmon resonance. The ability of these proteins to inhibit a broad range of interferons was investigated to identify potential adaptation to the human immune system. Furthermore, we demonstrate by Western blot and activity assays the expression of the type I interferon inhibitor during VARV and MPXV infections. These findings are relevant for the design of new vaccines and therapeutics to smallpox and emergent virulent orthopoxviruses because the type I interferon-binding protein is a major virulence factor in animal models, vaccination with this protein induces protective immunity, and its neutralization prevents disease progression.—Fernández de Marco, M. M., Alejo, A., Hudson, P., Damon, I. K., Alcami, A. The highly virulent variola and monkeypox viruses express secreted inhibitors of type I interferon. PMID:20019241

Real-time PCR to identify variola virus or other human pathogenic orthopox viruses.

PubMed

Scaramozzino, Natale; Ferrier-Rembert, Audrey; Favier, Anne-Laure; Rothlisberger, Corinne; Richard, Stéphane; Crance, Jean-Marc; Meyer, Hermann; Garin, Daniel

2007-04-01

Variola virus (family Poxviridae, genus Orthopoxvirus) and the closely related cowpox, vaccinia, and monkeypox viruses can infect humans. Efforts are mounting to replenish the smallpox vaccine stocks, optimize diagnostic methods for poxviruses, and develop new antivirals against smallpox, because it is feared that variola virus might be used as a weapon of bioterrorism. We developed an assay for the detection of variola virus DNA. The assay is based on TaqMan chemistry targeting the 14-kD protein gene. For the 1st stage of the assay we used genus consensus primers and a mixture of 2 probes (14-kD POX and 14-kD VAR) spanning the 14-kD protein-encoding gene for detection of all human pathogenic orthopoxviruses. We then tested positive samples with the specific orthopoxvirus-specific probe 14-kD POX to identify monkeypox, cowpox, and vaccinia viruses and with the 14-kD VAR probe to identify variola viruses. The assay was established on 4 different PCR cycler platforms. It was assessed in a study with 85 different orthopoxvirus species and strains that included variola, camelpox, cowpox, monkeypox, and vaccinia viruses at concentrations ranging from 100 ng/L to 1 microg/L. The assay detected as little as 0.05 fg of DNA, corresponding to 25 copies of DNA, and enabled differentiation of variola virus from the other orthopoxviruses. This real-time PCR assay provides a rapid method for the early detection and differentiation of smallpox and other human pathogenic orthopoxvirus infections.

Anticipating smallpox and monkeypox outbreaks: complications of the smallpox vaccine.

PubMed

Abrahams, Brian C; Kaufman, David M

2004-09-01

The recent outbreak in the Midwest of monkeypox, as well as the continued fears of a terrorist-induced epidemic of smallpox, prompted the authors' review of the literature regarding past and current experiences with smallpox vaccination. The smallpox vaccine, which is highly effective in preventing the spread of both these orthopoxvirus infectious illnesses, might be administered to numerous health care workers and, in the event of a smallpox attack, millions of other citizens. However, vaccinees would be at risk for several vaccine-related neurologic complications. According to prior reports, neurologic complications have occurred in 2.5 per million US individuals, with the most common being postvaccinal encephalomyelitis (PVEM). In older children and adults, PVEM causes stupor and coma, seizures, paraparesis, and other neurologic and mental abnormalities, and, in 16% of cases, permanent neurologic sequelae. The overall mortality rate of neurologic complications is approximately 1.5 per million vaccinees. Risk factors for PVEM were age younger than 1 year and no previous smallpox vaccination, but not a prior episode of PVEM or other preexisting neurologic illnesses. Neither the current smallpox vaccination campaigns in Israel nor the one in the United States has had comparable complications, but the US campaign has been associated with myocarditis and myopericarditis. Although the potential neurologic complications of the smallpox vaccine must be weighed against the threat of monkeypox and smallpox, current experience with vaccination suggests it carries a very low risk of neurologic complications and does not lead to exacerbations of chronic neurologic illnesses.

Late Pan-African and early Mesozoic brittle compressions in East and Central Africa: lithospheric deformation within the Congo-Tanzania Cratonic area

NASA Astrophysics Data System (ADS)

Delvaux, D.; Kipata, M. L.; Macheyeki, A. S.

2012-04-01

Tectonic reconstructions leading to the formation of the Central-African part of Gondwana have so far not much taken into account constraints provided by the evolution of brittle structures and related stress field. This is largely because little is known on continental brittle deformation in Equatorial Africa before the onset of the Mesozoic Central-African and Late Cenozoic East-African rifts. We present a synthesis of fault-kinematic data and paleostress inversion results from field surveys covering parts of Tanzania, Zambia and the Democratic Republic of Congo. It is based on investigations along the eastern margin of the Tanzanian craton, in the Ubendian belt between the Tanzanian craton and Bangweulu block, in the Lufilian Arc between the Kalahari and Congo cratons and along the Congo intracratonic basin. Paleostress tensors were computed for a substantial database by interactive stress tensor inversion and data subset separation, and the relative succession of major brittle events established. Two of them appear to be of regional importance and could be traced from one region to the other. The oldest one is the first brittle event recorded after the paroxysm of the Terminal Pan-African event that led to the amalgamation Gondwana at the Precambrian-Cambrian transition. It is related to compressional deformation with horizontal stress trajectories fluctuating from an E-W compression in Central Tanzania to NE-SW in the Ubende belt and Lufilian Arc. The second event is a transpressional inversion with a consistent NW-SE compression that we relate to the far-field effects of the active margin south of Gondwana during the late Triassic - early Jurassic.

A new strain of Crimean-Congo hemorrhagic fever virus isolated from Xinjiang, China.

PubMed

Guo, Rong; Shen, Shu; Zhang, Yanfang; Shi, Junming; Su, Zhengyuan; Liu, Dan; Liu, Jinliang; Yang, Juan; Wang, Qiguo; Hu, Zhihong; Zhang, Yujiang; Deng, Fei

2017-02-01

Crimean-Congo hemorrhagic fever virus (CCHFV) is a highly pathogenic tick-borne virus with a fatality rate of up to 50% in humans. CCHFV is widely distributed in countries around the world. Outbreaks of CCHFV infection in humans have occurred in prior years in Xinjiang Province, China. Epidemiological surveys have detected CCHFV RNA in ticks and animals; however, few isolates were identified. In this study, we identified and isolated a new CCHFV strain from Hyalomma asiaticum asiaticum ticks collected from north of Tarim Basin in Xinjiang, China. A preliminary investigation of infection and antigens expression of CCHFV was performed in newborn mice. The target tissues for CCHFV replication in newborn mice were identified. The analysis of the phylogenetic relationships with other Chinese strains suggested that diverse genotypes of CCHFV have circulated in Xinjiang for years. These findings provide important insights into our understanding of CCHFV infection and evolution as well as disease prevention and control for local residents.

La formation de l'inkisi (Supergroupe ouest-congolien) en Afrique centrale (Congo et Bas-Zaïre): un delta d'âge Paléozoïque comblant un bassin en extension

NASA Astrophysics Data System (ADS)

Alvarez, Philippe; Maurin, Jean-Christophe; Vicat, Jean-Paul

1995-02-01

The Inkisi Formation (West Congolian Supergroup) corresponds to a large deltaic body, which extends through Congo, Lower Zaire and Angola. In the Congo and Lower Zaire areas, the lower part of this formation is characterized by a fluvial conglomerate with elliptic pebbles. The red arkosic, channelized series from the Brazzaville-Kinshasa area involves delta plain distributary channels and delta front sequences. The transport direction of continental material is from north to south and the source area is the Chaillu basement. Glacial quartzitic pebbles are probably reworked from the fluvio-lacustrine Upper Diamictite Formation. The classical subdivisions of the Inkisi Formation - basal conglomerate (I 0), Lower part (I 1) and Upper part (I 2) - are not used. These subdivisions correspond to a fluvial conglomerate and to delta front and delta plain facies. The coastal onlap progressively covered the conglomerate and the distributary channels in the delta plain was prograding onto the delta front. The prodelta sequence could correspond to the Upper level of the Mpioka molassic Formation. The Inkisi delta was on the northern edge of an extensional basin controlled by NE-SW normal faults. The extension phase is dearly post Pan-African and occurred during the Palaeozoic, probably in relation to the Permian Karoo phase, and is also known in Angola.

T Cell Inactivation by Poxviral B22 Family Proteins Increases Viral Virulence

PubMed Central

Alzhanova, Dina; Hammarlund, Erika; Reed, Jason; Meermeier, Erin; Rawlings, Stephanie; Ray, Caroline A.; Edwards, David M.; Bimber, Ben; Legasse, Alfred; Planer, Shannon; Sprague, Jerald; Axthelm, Michael K.; Pickup, David J.; Lewinsohn, David M.; Gold, Marielle C.; Wong, Scott W.; Sacha, Jonah B.; Slifka, Mark K.; Früh, Klaus

2014-01-01

Infections with monkeypox, cowpox and weaponized variola virus remain a threat to the increasingly unvaccinated human population, but little is known about their mechanisms of virulence and immune evasion. We now demonstrate that B22 proteins, encoded by the largest genes of these viruses, render human T cells unresponsive to stimulation of the T cell receptor by MHC-dependent antigen presentation or by MHC-independent stimulation. In contrast, stimuli that bypass TCR-signaling are not inhibited. In a non-human primate model of monkeypox, virus lacking the B22R homologue (MPXVΔ197) caused only mild disease with lower viremia and cutaneous pox lesions compared to wild type MPXV which caused high viremia, morbidity and mortality. Since MPXVΔ197-infected animals displayed accelerated T cell responses and less T cell dysregulation than MPXV US2003, we conclude that B22 family proteins cause viral virulence by suppressing T cell control of viral dissemination. PMID:24832205

T cell inactivation by poxviral B22 family proteins increases viral virulence.

PubMed

Alzhanova, Dina; Hammarlund, Erika; Reed, Jason; Meermeier, Erin; Rawlings, Stephanie; Ray, Caroline A; Edwards, David M; Bimber, Ben; Legasse, Alfred; Planer, Shannon; Sprague, Jerald; Axthelm, Michael K; Pickup, David J; Lewinsohn, David M; Gold, Marielle C; Wong, Scott W; Sacha, Jonah B; Slifka, Mark K; Früh, Klaus

2014-05-01

Infections with monkeypox, cowpox and weaponized variola virus remain a threat to the increasingly unvaccinated human population, but little is known about their mechanisms of virulence and immune evasion. We now demonstrate that B22 proteins, encoded by the largest genes of these viruses, render human T cells unresponsive to stimulation of the T cell receptor by MHC-dependent antigen presentation or by MHC-independent stimulation. In contrast, stimuli that bypass TCR-signaling are not inhibited. In a non-human primate model of monkeypox, virus lacking the B22R homologue (MPXVΔ197) caused only mild disease with lower viremia and cutaneous pox lesions compared to wild type MPXV which caused high viremia, morbidity and mortality. Since MPXVΔ197-infected animals displayed accelerated T cell responses and less T cell dysregulation than MPXV US2003, we conclude that B22 family proteins cause viral virulence by suppressing T cell control of viral dissemination.

Attenuation of monkeypox virus by deletion of genomic regions

USGS Publications Warehouse

Lopera, Juan G.; Falendysz, Elizabeth A.; Rocke, Tonie E.; Osorio, Jorge E.

2015-01-01

Monkeypox virus (MPXV) is an emerging pathogen from Africa that causes disease similar to smallpox. Two clades with different geographic distributions and virulence have been described. Here, we utilized bioinformatic tools to identify genomic regions in MPXV containing multiple virulence genes and explored their roles in pathogenicity; two selected regions were then deleted singularly or in combination. In vitro and in vivostudies indicated that these regions play a significant role in MPXV replication, tissue spread, and mortality in mice. Interestingly, while deletion of either region led to decreased virulence in mice, one region had no effect on in vitro replication. Deletion of both regions simultaneously also reduced cell culture replication and significantly increased the attenuation in vivo over either single deletion. Attenuated MPXV with genomic deletions present a safe and efficacious tool in the study of MPX pathogenesis and in the identification of genetic factors associated with virulence.

Occupational Risks during a Monkeypox Outbreak, Wisconsin, 2003

PubMed Central

Sotir, Mark J.; Williams, Carl J.; Kazmierczak, James J.; Wegner, Mark V.; Rausch, Darren; Graham, Mary Beth; Foldy, Seth L.; Wolters, Mat; Damon, Inger K.; Karem, Kevin L.; Davis, Jeffrey P.

2007-01-01

We determined factors associated with occupational transmission in Wisconsin during the 2003 outbreak of prairie dog–associated monkeypox virus infections. Our investigation included active contact surveillance, exposure-related interviews, and a veterinary facility cohort study. We identified 19 confirmed, 5 probable, and 3 suspected cases. Rash, headache, sweats, and fever were reported by >80% of patients. Occupationally transmitted infections occurred in 12 veterinary staff, 2 pet store employees, and 2 animal distributors. The following were associated with illness: working directly with animal care (p = 0.002), being involved in prairie dog examination, caring for an animal within 6 feet of an ill prairie dog (p = 0.03), feeding an ill prairie dog (p = 0.002), and using an antihistamine (p = 0.04). Having never handled an ill prairie dog (p = 0.004) was protective. Veterinary staff used personal protective equipment sporadically. Our findings underscore the importance of standard veterinary infection-control guidelines. PMID:17953084

Attenuation of monkeypox virus by deletion of genomic regions.

PubMed

Lopera, Juan G; Falendysz, Elizabeth A; Rocke, Tonie E; Osorio, Jorge E

2015-01-15

Monkeypox virus (MPXV) is an emerging pathogen from Africa that causes disease similar to smallpox. Two clades with different geographic distributions and virulence have been described. Here, we utilized bioinformatic tools to identify genomic regions in MPXV containing multiple virulence genes and explored their roles in pathogenicity; two selected regions were then deleted singularly or in combination. In vitro and in vivo studies indicated that these regions play a significant role in MPXV replication, tissue spread, and mortality in mice. Interestingly, while deletion of either region led to decreased virulence in mice, one region had no effect on in vitro replication. Deletion of both regions simultaneously also reduced cell culture replication and significantly increased the attenuation in vivo over either single deletion. Attenuated MPXV with genomic deletions present a safe and efficacious tool in the study of MPX pathogenesis and in the identification of genetic factors associated with virulence. Copyright © 2014 Elsevier Inc. All rights reserved.

Orthopoxvirus variola infection of Cynomys ludovicianus (North American black tailed prairie dog).

PubMed

Carroll, Darin S; Olson, Victoria A; Smith, Scott K; Braden, Zach H; Patel, Nishi; Abel, Jason; Li, Yu; Damon, Inger K; Karem, Kevin L

2013-09-01

Since the eradication of Smallpox, researchers have attempted to study Orthopoxvirus pathogenesis and immunity in animal models in order to correlate results human smallpox. A solely human pathogen, Orthopoxvirus variola fails to produce authentic smallpox illness in any other animal species tested to date. In 2003, an outbreak in the USA of Orthopoxvirus monkeypox, revealed the susceptibility of the North American black-tailed prairie dog (Cynomys ludovicianus) to infection and fulminate disease. Prairie dogs infected with Orthopoxvirus monkeypox present with a clinical scenario similar to ordinary smallpox, including prodrome, rash, and high mortality. This study examines if Black-tailed prairie dogs can become infected with O. variola and serve as a surrogate model for the study of human smallpox disease. Substantive evidence of infection is found in immunological seroconversion of animals to either intranasal or intradermal challenges with O. variola, but in the absence of overt illness. Published by Elsevier Inc.

Molecular Smallpox Vaccine Delivered by Alphavirus Replicons Elicits Protective Immunity in Mice and Non-human Primates

PubMed Central

Hooper, Jay W.; Ferro, Anthony M.; Golden, Joseph W.; Silvera, Peter; Dudek, Jeanne; Alterson, Kim; Custer, Max; Rivers, Bryan; Morris, John; Owens, Gary; Smith, Jonathan F.; Kamrud, Kurt I.

2009-01-01

Naturally occurring smallpox was eradicated as a result of successful vaccination campaigns during the 1960s and 70s. Because of its highly contagious nature and high mortality rate, smallpox has significant potential as a biological weapon. Unfortunately, the current vaccine for orthopoxviruses is contraindicated for large portions of the population. Thus, there is a need for new, safe, and effective orthopoxvirus vaccines. Alphavirus replicon vectors, derived from strains of Venezuelan equine encephalitis virus, are being used to develop alternatives to the current smallpox vaccine. Here, we demonstrated that virus-like replicon particles (VRP) expressing the vaccinia virus A33R, B5R, A27L, and L1R genes elicited protective immunity in mice comparable to vaccination with live-vaccinia virus. Furthermore, cynomolgus macaques vaccinated with a combination of the four poxvirus VRPs (4pox-VRP) developed antibody responses to each antigen. These antibody responses were able to neutralize and inhibit the spread of both vaccinia virus and monkeypox virus. Macaques vaccinated with 4pox-VRP, flu HA VRP (negative control), or live-vaccinia virus (positive control) were challenged intravenously with 5 × 106 PFU of monkeypox virus 1 month after the second VRP vaccination. Four of the six negative control animals succumbed to monkeypox and the remaining two animals demonstrated either severe or grave disease. Importantly, all 10 macaques vaccinated with the 4pox-VRP vaccine survived without developing severe disease. These findings revealed that a single-boost VRP smallpox vaccine shows promise as a safe alternative to the currently licensed live-vaccinia virus smallpox vaccine. PMID:19833247

Antiviral treatment is more effective than smallpox vaccination upon lethal monkeypox virus infection.

PubMed

Stittelaar, Koert J; Neyts, Johan; Naesens, Lieve; van Amerongen, Geert; van Lavieren, Rob F; Holý, Antonin; De Clercq, Erik; Niesters, Hubert G M; Fries, Edwin; Maas, Chantal; Mulder, Paul G H; van der Zeijst, Ben A M; Osterhaus, Albert D M E

2006-02-09

There is concern that variola virus, the aetiological agent of smallpox, may be used as a biological weapon. For this reason several countries are now stockpiling (vaccinia virus-based) smallpox vaccine. Although the preventive use of smallpox vaccination has been well documented, little is known about its efficacy when used after exposure to the virus. Here we compare the effectiveness of (1) post-exposure smallpox vaccination and (2) antiviral treatment with either cidofovir (also called HPMPC or Vistide) or with a related acyclic nucleoside phosphonate analogue (HPMPO-DAPy) after lethal intratracheal infection of cynomolgus monkeys (Macaca fascicularis) with monkeypox virus (MPXV). MPXV causes a disease similar to human smallpox and this animal model can be used to measure differences in the protective efficacies of classical and new-generation candidate smallpox vaccines. We show that initiation of antiviral treatment 24 h after lethal intratracheal MPXV infection, using either of the antiviral agents and applying various systemic treatment regimens, resulted in significantly reduced mortality and reduced numbers of cutaneous monkeypox lesions. In contrast, when monkeys were vaccinated 24 h after MPXV infection, using a standard human dose of a currently recommended smallpox vaccine (Elstree-RIVM), no significant reduction in mortality was observed. When antiviral therapy was terminated 13 days after infection, all surviving animals had virus-specific serum antibodies and antiviral T lymphocytes. These data show that adequate preparedness for a biological threat involving smallpox should include the possibility of treating exposed individuals with antiviral compounds such as cidofovir or other selective anti-poxvirus drugs.

Variability in aerobic methane oxidation over the past 1.2 Myrs recorded in microbial biomarker signatures from Congo fan sediments

NASA Astrophysics Data System (ADS)

Talbot, Helen M.; Handley, Luke; Spencer-Jones, Charlotte L.; Dinga, Bienvenu Jean; Schefuß, Enno; Mann, Paul J.; Poulsen, John R.; Spencer, Robert G. M.; Wabakanghanzi, Jose N.; Wagner, Thomas

2014-05-01

Methane (CH4) is a strong greenhouse gas known to have perturbed global climate in the past, especially when released in large quantities over short time periods from continental or marine sources. It is therefore crucial to understand and, if possible, quantify the individual and combined response of these variable methane sources to natural climate variability. However, past changes in the stability of greenhouse gas reservoirs remain uncertain and poorly constrained by geological evidence. Here, we present a record from the Congo fan of a highly specific bacteriohopanepolyol (BHP) biomarker for aerobic methane oxidation (AMO), 35-aminobacteriohopane-30,31,32,33,34-pentol (aminopentol), that identifies discrete periods of increased AMO as far back as 1.2 Ma. Fluctuations in the concentration of aminopentol, and other 35-aminoBHPs, follow a pattern that correlates with late Quaternary glacial-interglacial climate cycles, with highest concentrations during warm periods. We discuss possible sources of aminopentol, and the methane consumed by the precursor methanotrophs, within the context of the Congo River setting, including supply of methane oxidation markers from terrestrial watersheds and/or marine sources (gas hydrate and/or deep subsurface gas reservoir). Compound-specific carbon isotope values of -30‰ to -40‰ for BHPs in ODP 1075 and strong similarities between the BHP signature of the core and surface sediments from the Congo estuary and floodplain wetlands from the interior of the Congo River Basin, support a methanotrophic and likely terrigenous origin of the 35-aminoBHPs found in the fan sediments. This new evidence supports a causal connection between marine sediment BHP records of tropical deep sea fans and wetland settings in the feeding river catchments, and thus tropical continental hydrology. Further research is needed to better constrain the different sources and pathways of methane emission. However, this study identifies the large potential of aminoBHPs, in particular aminopentol, to trace and, once better calibrated and understood, quantify past methane sources and fluxes from terrestrial and potentially also marine sources.

A Multiplex PCR/LDR Assay for the Simultaneous Identification of Category A Infectious Pathogens: Agents of Viral Hemorrhagic Fever and Variola Virus

PubMed Central

Das, Sanchita; Rundell, Mark S.; Mirza, Aashiq H.; Pingle, Maneesh R.; Shigyo, Kristi; Garrison, Aura R.; Paragas, Jason; Smith, Scott K.; Olson, Victoria A.; Larone, Davise H.; Spitzer, Eric D.; Barany, Francis; Golightly, Linnie M.

2015-01-01

CDC designated category A infectious agents pose a major risk to national security and require special action for public health preparedness. They include viruses that cause viral hemorrhagic fever (VHF) syndrome as well as variola virus, the agent of smallpox. VHF is characterized by hemorrhage and fever with multi-organ failure leading to high morbidity and mortality. Smallpox, a prior scourge, has been eradicated for decades, making it a particularly serious threat if released nefariously in the essentially non-immune world population. Early detection of the causative agents, and the ability to distinguish them from other pathogens, is essential to contain outbreaks, implement proper control measures, and prevent morbidity and mortality. We have developed a multiplex detection assay that uses several species-specific PCR primers to generate amplicons from multiple pathogens; these are then targeted in a ligase detection reaction (LDR). The resultant fluorescently-labeled ligation products are detected on a universal array enabling simultaneous identification of the pathogens. The assay was evaluated on 32 different isolates associated with VHF (ebolavirus, marburgvirus, Crimean Congo hemorrhagic fever virus, Lassa fever virus, Rift Valley fever virus, Dengue virus, and Yellow fever virus) as well as variola virus and vaccinia virus (the agent of smallpox and its vaccine strain, respectively). The assay was able to detect all viruses tested, including 8 sequences representative of different variola virus strains from the CDC repository. It does not cross react with other emerging zoonoses such as monkeypox virus or cowpox virus, or six flaviviruses tested (St. Louis encephalitis virus, Murray Valley encephalitis virus, Powassan virus, Tick-borne encephalitis virus, West Nile virus and Japanese encephalitis virus). PMID:26381398

A Multiplex PCR/LDR Assay for the Simultaneous Identification of Category A Infectious Pathogens: Agents of Viral Hemorrhagic Fever and Variola Virus.

PubMed

Das, Sanchita; Rundell, Mark S; Mirza, Aashiq H; Pingle, Maneesh R; Shigyo, Kristi; Garrison, Aura R; Paragas, Jason; Smith, Scott K; Olson, Victoria A; Larone, Davise H; Spitzer, Eric D; Barany, Francis; Golightly, Linnie M

2015-01-01

CDC designated category A infectious agents pose a major risk to national security and require special action for public health preparedness. They include viruses that cause viral hemorrhagic fever (VHF) syndrome as well as variola virus, the agent of smallpox. VHF is characterized by hemorrhage and fever with multi-organ failure leading to high morbidity and mortality. Smallpox, a prior scourge, has been eradicated for decades, making it a particularly serious threat if released nefariously in the essentially non-immune world population. Early detection of the causative agents, and the ability to distinguish them from other pathogens, is essential to contain outbreaks, implement proper control measures, and prevent morbidity and mortality. We have developed a multiplex detection assay that uses several species-specific PCR primers to generate amplicons from multiple pathogens; these are then targeted in a ligase detection reaction (LDR). The resultant fluorescently-labeled ligation products are detected on a universal array enabling simultaneous identification of the pathogens. The assay was evaluated on 32 different isolates associated with VHF (ebolavirus, marburgvirus, Crimean Congo hemorrhagic fever virus, Lassa fever virus, Rift Valley fever virus, Dengue virus, and Yellow fever virus) as well as variola virus and vaccinia virus (the agent of smallpox and its vaccine strain, respectively). The assay was able to detect all viruses tested, including 8 sequences representative of different variola virus strains from the CDC repository. It does not cross react with other emerging zoonoses such as monkeypox virus or cowpox virus, or six flaviviruses tested (St. Louis encephalitis virus, Murray Valley encephalitis virus, Powassan virus, Tick-borne encephalitis virus, West Nile virus and Japanese encephalitis virus).

Characterization of Monkeypox virus infection in African rope squirrels (Funisciurus sp.)

USGS Publications Warehouse

Falendysz, Elizabeth; Lopera, Juan G.; Doty, Jeffrey B.; Nakazawa, Yoshinori J.; Crill, Colleen; Lorenzsonn, Faye; Kalemba, Lem's N.; Ronderos, Monica; Meija, Andres; Malekani, Jean M.; Karem, Kevin L.; Caroll, Darrin; Osorio, Jorge E.; Rocke, Tonie E.

2017-01-01

Monkeypox (MPX) is a zoonotic disease endemic in Central and West Africa and is caused by Monkeypox virus (MPXV), the most virulent Orthopoxvirus affecting humans since the eradication of Variola virus (VARV). Many aspects of the MPXV transmission cycle, including the natural host of the virus, remain unknown. African rope squirrels (Funisciurus spp.) are considered potential reservoirs of MPXV, as serosurveillance data in Central Africa has confirmed the circulation of the virus in these rodent species [1,2]. In order to understand the tissue tropism and clinical signs associated with infection with MPXV in these species, wild-caught rope squirrels were experimentally infected via intranasal and intradermal exposure with a recombinant MPXV strain from Central Africa engineered to express the luciferase gene. After infection, we monitored viral replication and shedding via in vivo bioluminescent imaging, viral culture and real time PCR. MPXV infection in African rope squirrels caused mortality and moderate to severe morbidity, with clinical signs including pox lesions in the skin, eyes, mouth and nose, dyspnea, and profuse nasal discharge. Both intranasal and intradermal exposures induced high levels of viremia, fast systemic spread, and long periods of viral shedding. Shedding and luminescence peaked at day 6 post infection and was still detectable after 15 days. Interestingly, one sentinel animal, housed in the same room but in a separate cage, also developed severe MPX disease and was euthanized. This study indicates that MPXV causes significant pathology in African rope squirrels and infected rope squirrels shed large quantities of virus, supporting their role as a potential source of MPXV transmission to humans and other animals in endemic MPX regions.

Characterization of Monkeypox virus infection in African rope squirrels (Funisciurus sp.).

PubMed

Falendysz, Elizabeth A; Lopera, Juan G; Doty, Jeffrey B; Nakazawa, Yoshinori; Crill, Colleen; Lorenzsonn, Faye; Kalemba, Lem's N; Ronderos, Monica D; Mejia, Andres; Malekani, Jean M; Karem, Kevin; Carroll, Darin S; Osorio, Jorge E; Rocke, Tonie E

2017-08-01

Monkeypox (MPX) is a zoonotic disease endemic in Central and West Africa and is caused by Monkeypox virus (MPXV), the most virulent Orthopoxvirus affecting humans since the eradication of Variola virus (VARV). Many aspects of the MPXV transmission cycle, including the natural host of the virus, remain unknown. African rope squirrels (Funisciurus spp.) are considered potential reservoirs of MPXV, as serosurveillance data in Central Africa has confirmed the circulation of the virus in these rodent species [1,2]. In order to understand the tissue tropism and clinical signs associated with infection with MPXV in these species, wild-caught rope squirrels were experimentally infected via intranasal and intradermal exposure with a recombinant MPXV strain from Central Africa engineered to express the luciferase gene. After infection, we monitored viral replication and shedding via in vivo bioluminescent imaging, viral culture and real time PCR. MPXV infection in African rope squirrels caused mortality and moderate to severe morbidity, with clinical signs including pox lesions in the skin, eyes, mouth and nose, dyspnea, and profuse nasal discharge. Both intranasal and intradermal exposures induced high levels of viremia, fast systemic spread, and long periods of viral shedding. Shedding and luminescence peaked at day 6 post infection and was still detectable after 15 days. Interestingly, one sentinel animal, housed in the same room but in a separate cage, also developed severe MPX disease and was euthanized. This study indicates that MPXV causes significant pathology in African rope squirrels and infected rope squirrels shed large quantities of virus, supporting their role as a potential source of MPXV transmission to humans and other animals in endemic MPX regions.

Sero-epidemiological survey of Crimean-Congo hemorrhagic fever virus in Tunisia

PubMed Central

Wasfi, Fares; Dowall, Stuart; Ghabbari, Tayssir; Bosworth, Andrew; Chakroun, Mohamed; Varghese, Anitha; Tiouiri, Hanene; Ben Jemaa, Mounir; Znazen, Abir; Hewson, Roger; Zhioua, Elyes; Letaief, Amel

2016-01-01

Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne disease associated with a high case fatality rate and transmitted mainly by Hyalomma marginatum. The geographical distribution of H. marginatum covers most of the Western Mediterranean basin. We aimed to investigate whether CCHF virus (CCHFv) is circulating in Tunisia. Samples from unexplained acute febrile patients (n = 181) and a high risk group of humans, mainly slaughter workers (n = 38), were collected in the summer of 2014 and analyzed for exposure to CCHFv using serological tests and real-time RT-PCR. Ticks were collected from Northern and Southern Tunisia during May–June 2014 and examined for the presence of CCHFv by real-time RT-PCR. Of the 181 febrile patients, 5 showed only high titers of IgM suggesting a recent exposure to CCHFv. Among 38 slaughter workers, 2 had IgG anti-CCHFv responses yielding a seroprevalence of 5.2%. No CCHFv was detected in ticks and sera. Our results provide evidence of human exposure to CCHFv in Tunisia. PMID:26956221

Design complexity in termite-fishing tools of chimpanzees (Pan troglodytes)

PubMed Central

Sanz, Crickette; Call, Josep; Morgan, David

2009-01-01

Adopting the approach taken with New Caledonian crows (Corvus moneduloides), we present evidence of design complexity in one of the termite-fishing tools of chimpanzees (Pan troglodytes) in the Goualougo Triangle, Republic of Congo. Prior to termite fishing, chimpanzees applied a set of deliberate, distinguishable actions to modify herb stems to fashion a brush-tipped probe, which is different from the form of fishing tools used by chimpanzees in East and West Africa. This means that ‘brush-tipped fishing probes’, unlike ‘brush sticks’, are not a by-product of use but a deliberate design feature absent in other chimpanzee populations. The specialized modifications to prepare the tool for termite fishing, measures taken to repair non-functional brushes and appropriate orientation of the modified end suggest that these wild chimpanzees are attentive to tool modifications. We also conducted experimental trials that showed that a brush-tipped probe is more effective in gathering insects than an unmodified fishing probe. Based on these findings, we suggest that chimpanzees in the Congo Basin have developed an improved fishing probe design. PMID:19324641

Sero-epidemiological survey of Crimean-Congo hemorrhagic fever virus in Tunisia.

PubMed

Wasfi, Fares; Dowall, Stuart; Ghabbari, Tayssir; Bosworth, Andrew; Chakroun, Mohamed; Varghese, Anitha; Tiouiri, Hanene; Ben Jemaa, Mounir; Znazen, Abir; Hewson, Roger; Zhioua, Elyes; Letaief, Amel

2016-01-01

Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne disease associated with a high case fatality rate and transmitted mainly by Hyalomma marginatum. The geographical distribution of H. marginatum covers most of the Western Mediterranean basin. We aimed to investigate whether CCHF virus (CCHFv) is circulating in Tunisia. Samples from unexplained acute febrile patients (n = 181) and a high risk group of humans, mainly slaughter workers (n = 38), were collected in the summer of 2014 and analyzed for exposure to CCHFv using serological tests and real-time RT-PCR. Ticks were collected from Northern and Southern Tunisia during May-June 2014 and examined for the presence of CCHFv by real-time RT-PCR. Of the 181 febrile patients, 5 showed only high titers of IgM suggesting a recent exposure to CCHFv. Among 38 slaughter workers, 2 had IgG anti-CCHFv responses yielding a seroprevalence of 5.2%. No CCHFv was detected in ticks and sera. Our results provide evidence of human exposure to CCHFv in Tunisia. © F. Wasfi et al., published by EDP Sciences, 2016.

Genesis of sediment-hosted stratiform copper cobalt deposits, central African Copperbelt

NASA Astrophysics Data System (ADS)

Cailteux, J. L. H.; Kampunzu, A. B.; Lerouge, C.; Kaputo, A. K.; Milesi, J. P.

2005-07-01

The Neoproterozoic central African Copperbelt is one of the greatest sediment-hosted stratiform Cu-Co provinces in the world, totalling 140 Mt copper and 6 Mt cobalt and including several world-class deposits (⩾10 Mt copper). The origin of Cu-Co mineralisation in this province remains speculative, with the debate centred around syngenetic-diagenetic and hydrothermal-diagenetic hypotheses. The regional distribution of metals indicates that most of the cobalt-rich copper deposits are hosted in dolomites and dolomitic shales forming allochthonous units exposed in Congo and known as Congolese facies of the Katangan sedimentary succession (average Co:Cu = 1:13). The highest Co:Cu ratio (up to 3:1) occurs in ore deposits located along the southern structural block of the Lufilian Arc. The predominantly siliciclastic Zambian facies, exposed in Zambia and in SE Congo, forms para-autochthonous sedimentary units hosting ore deposits characterized by lower a Co:Cu ratio (average 1:57). Transitional lithofacies in Zambia (e.g. Baluba, Mindola) and in Congo (e.g. Lubembe) indicate a gradual transition in the Katangan basin during the deposition of laterally correlative clastic and carbonate sedimentary rocks exposed in Zambia and in Congo, and are marked by Co:Cu ratios in the range 1:15. The main Cu-Co orebodies occur at the base of the Mines/Musoshi Subgroup, which is characterized by evaporitic intertidal-supratidal sedimentary rocks. All additional lenticular orebodies known in the upper part of the Mines/Musoshi Subgroup are hosted in similar sedimentary rocks, suggesting highly favourable conditions for the ore genesis in particular sedimentary environments. Pre-lithification sedimentary structures affecting disseminated sulphides indicate that metals were deposited before compaction and consolidation of the host sediment. The ore parageneses indicate several generations of sulphides marking syngenetic, early diagenetic and late diagenetic processes. Sulphur isotopic data on sulphides suggest the derivation of sulphur essentially from the bacterial reduction of seawater sulphates. The mineralizing brines were generated from sea water in sabkhas or hypersaline lagoons during the deposition of the host rocks. Changes of Eh-pH and salinity probably were critical for concentrating copper-cobalt and nickel mineralisation. Compressional tectonic and related metamorphic processes and supergene enrichment have played variable roles in the remobilisation and upgrading of the primary mineralisation. There is no evidence to support models assuming that metals originated from: (1) Katangan igneous rocks and related hydrothermal processes or; (2) leaching of red beds underlying the orebodies. The metal sources are pre-Katangan continental rocks, especially the Palaeoproterozoic low-grade porphyry copper deposits known in the Bangweulu block and subsidiary Cu-Co-Ni deposits/occurrences in the Archaean rocks of the Zimbabwe craton. These two sources contain low grade ore deposits portraying the peculiar metal association (Cu, Co, Ni, U, Cr, Au, Ag, PGE) recorded in the Katangan sediment-hosted ore deposits. Metals were transported into the basin dissolved in water. The stratiform deposits of Congo and Zambia display features indicating that syngenetic and early diagenetic processes controlled the formation of the Neoproterozoic Copperbelt of central Africa.

Effects of logging on roadless space in intact forest landscapes of the Congo Basin.

PubMed

Kleinschroth, Fritz; Healey, John R; Gourlet-Fleury, Sylvie; Mortier, Frédéric; Stoica, Radu S

2017-04-01

Forest degradation in the tropics is often associated with roads built for selective logging. The protection of intact forest landscapes (IFL) that are not accessible by roads is high on the biodiversity conservation agenda and a challenge for logging concessions certified by the Forest Stewardship Council (FSC). A frequently advocated conservation objective is to maximize the retention of roadless space, a concept that is based on distance to the nearest road from any point. We developed a novel use of the empty-space function - a general statistical tool based on stochastic geometry and random sets theory - to calculate roadless space in a part of the Congo Basin where road networks have been expanding rapidly. We compared the temporal development of roadless space in certified and uncertified logging concessions inside and outside areas declared IFL in 2000. Inside IFLs, road-network expansion led to a decrease in roadless space by more than half from 1999 to 2007. After 2007, loss leveled out in most areas to close to 0 due to an equilibrium between newly built roads and abandoned roads that became revegetated. However, concessions in IFL certified by FSC since around 2007 continuously lost roadless space and reached a level comparable to all other concessions. Only national parks remained mostly roadless. We recommend that forest-management policies make the preservation of large connected forest areas a top priority by effectively monitoring - and limiting - the occupation of space by roads that are permanently accessible. © 2016 The Authors. Conservation Biology published by Wiley Periodicals, Inc. on behalf of Society for Conservation Biology.

The immunology of smallpox vaccines

PubMed Central

Kennedy, Richard B; Ovsyannikova, Inna G; Jacobson, Robert M; Poland, Gregory A

2010-01-01

In spite of the eradication of smallpox over 30 years ago; orthopox viruses such as smallpox and monkeypox remain serious public health threats both through the possibility of bioterrorism and the intentional release of smallpox and through natural outbreaks of emerging infectious diseases such as monkeypox. The eradication effort was largely made possible by the availability of an effective vaccine based on the immunologically cross-protective vaccinia virus. Although the concept of vaccination dates back to the late 1800s with Edward Jenner, it is only in the past decade that modern immunologic tools have been applied toward deciphering poxvirus immunity. Smallpox vaccines containing vaccinia virus elicit strong humoral and cellular immune responses that confer cross-protective immunity against variola virus for decades after immunization. Recent studies have focused on: establishing the longevity of poxvirus-specific immunity, defining key immune epitopes targeted by T and B cells, developing subunit-based vaccines, and developing genotypic and phenotypic immune response profiles that predict either vaccine response or adverse events following immunization. PMID:19524427

Cichlid Fishes in the Angolan Headwaters Region: Molecular Evidence of the Ichthyofaunal Contact between the Cuanza and Okavango-Zambezi Systems

PubMed Central

Musilová, Zuzana; Kalous, Lukáš; Petrtýl, Miloslav; Chaloupková, Petra

2013-01-01

The headwaters of five large African river basins flow through the Bié Plateau in Angola and still remain faunistically largely unexplored. We investigated fish fauna from the Cuanza and Okavango-Zambezi river systems from central Angola. We reconstructed molecular phylogenies of the most common cichlid species from the region, Tilapia sparrmanii and Serranochromis macrocephalus, using both mitochondrial and nuclear markers. We found evidence for ichthyofaunal contact and gene flow between the Cuanza and Okavango-Zambezi watersheds in the Bié Plateau in central Angola. Waterfalls and rapids also appeared to restrict genetic exchange among populations within the Cuanza basin. Further, we found that the Angolan Serranochromis cichlid fishes represent a monophyletic lineage with respect to other haplochromines, including the serranochromines from the Congo and Zambezi rivers. This study represents an important initial step in a biodiversity survey of this extremely poorly explored region, as well as giving further understanding to species distributions and gene flow both between and within river basins. PMID:23724120

Rewriting the Landform History of One of Africa's Three Largest Basins

NASA Technical Reports Server (NTRS)

Wilkinson, Justin

2014-01-01

The Kalahari Basin in southern Africa - one of the largest basins in Africa, along with the Congo and Chad basins - has attracted attention since David Livingstone traveled through the area in the 1840s. It is a semiarid desert with a large freshwater swampland known as the Okavango Swamp (150 km radius). This prominent megafan (a fan with radii >100 km), with its fingers of dark green forests projecting into the dun colors of the dunes of the Kalahari semi-desert, has been well photographed by astronauts over the years. The study area in the northern Kalahari basin is centered on the Okavango megafan of northwest Botswana, whose swampland has become well known as an African wildlife preserve of importance to biology and tourism alike. The Okavango River is unusual because it has deposited not one but two megafans along its course: the Okavango megafan and the Cubango megafan. The Okavango megafan is one of only three well-known megafans in Africa. Megafans on Earth were once thought to be rare, but recent research has documented 68 in Africa alone. Eleven megafans, plus three more candidates, have been documented in the area immediately surrounding the Okavango feature. These 11 megafans occupy the flattest and smoothest terrains adjacent to the neighboring upland and stand out as the darkest areas in the roughness map of the area. Megafan terrains occupy at least 200,000 sq km of the study area. The roughness map shown is based on an algorithm used first on Mars to quantify topographic roughness. Research of Earth's flattest terrains is just beginning with the aid of such maps, and it appears that these terrains are analogous to the flattest regions of Mars. Implications: 1. The variability in depositional style in each subbasin may apply Africa-wide: rift megafan length is dominated by rift width, whereas Owambo subbasin megafans are probably controlled by upland basin size; Zambezi subbasin megafans appear more like foreland basin types, with the position of the trunk river controlling size. 2. These perspectives were successfully applied to identify the largest megafan in the group (Cubango), a fan that was sufficiently overprinted by dunes and dry lakelets not to be detectable remotely. Such undertsanding can probably be applied on Mars, where Earth experience suggests megafans ought to exist. 3. Sweep angles of rivers on megafans drastically change the hydrology in some subbasins: when the Cubango and Kunene rivers were oriented to the Etosha Pan, it was probably a permanent water body. Now that the rivers are oriented away from the basin, 93 percent of the discharge area from the pan's northerly (main) source area is gone. 4. Biotic contact between major river systems was probably controlled by megafans situated on divides: various fish species that originated in the Congo basin are now found in the Upper Zambezi R., and vice versa, apparently because of river switching behavior on the Cassai megafan that has mediated migrations both to the south and the north.

Radar Mosaic of Africa

NASA Technical Reports Server (NTRS)

1999-01-01

This is an image of equatorial Africa, centered on the equator at longitude 15degrees east. This image is a mosaic of almost 4,000 separate images obtained in 1996 by the L-band imaging radar onboard the Japanese Earth Resources Satellite. Using radar to penetrate the persistent clouds prevalent in tropical forests, the Japanese Earth Resources Satellite was able for the first time to image at high resolution this continental scale region during single flooding seasons. The area shown covers about 7.4 million square kilometers (2.8 million square miles) of land surface, spans more than 5,000 kilometers(3,100 miles) east and west and some 2,000 kilometers (1,240 miles) north and south. North is up in this image. At the full resolution of the mosaic (100 meters or 330 feet), this image is more than 500 megabytes in size, and was processed from imagery totaling more than 60 gigabytes.

Central Africa was imaged twice in 1996, once between January and March, which is the major low-flood season in the Congo Basin, and once between October and November, which is the major high-flood season in the Congo Basin. The red color corresponds to the data from the low-flood season, the green to the high-flood season, and the blue to the 'texture' of the low-flood data. The forests appear green as a result, the flooded and palm forests, as well as urban areas, appear yellow, the ocean and lakes appear black, and savanna areas appear blue, black or green, depending on the savanna type, surface topography and other factors. The areas of the image that are black and white were mapped only between January and March 1996. In these areas, the black areas are savanna or open water, the gray are forests, and the white areas are flooded forests or urban areas. The Congo River dominates the middle of the image, where the nearby forests that are periodically flooded by the Congo and its tributaries stand out as yellow. The Nile River flows north from Lake Victoria in the middle right of the color portion of the mosaic.

This image is one of the products resulting from the Global Rain Forest Mapping project, a joint project between the National Space Development Agency of Japan, the Space Applications Institute of the Joint Research Centre of the European Commission, NASA's Jet Propulsion Laboratory and an international team of scientists. The goal of the Global Rain Forest Mapping mission is to map with the Japanese Earth Resources Satellite the world's tropical rain forests. The Japanese satellite was launched in 1992 by the National Space Development Agency of Japan and the Japanese Ministry of International Trade and Industry, with support from the Remote Sensing Technology Center of Japan.

Transforming Surface Water Hydrology Through SWOT Altimetry

NASA Astrophysics Data System (ADS)

Alsdorf, Douglas; Mognard, Nelly; Rodriguez, Ernesto

2013-09-01

SWOT will measure water surface elevations across rivers, lakes, wetlands, and reservoirs with a 120km wide swath using decimeter-scale pixels having centimetric-scale height accuracies. Nothing like this "water surface topography" has been collected on a consistent basis from any method. Thus, SWOT will provide a transformative measurement for global hydrology. Storage change measurements from SWOT are expected to have an error of 10% or better for 250m2 and larger water bodies. Discharge estimation is complicated by the lack of channel bathymetric knowledge. Nevertheless, two model-based studies of the Ohio River suggest SWOT discharge errors will be 10%. Important questions will be addressed via SWOT measurements, e.g., (1) What is the water balance of the Congo Basin and indeed of any basin? (2) Where does a wetland receive its water: from upland runoff or from an adjacent river? (3) What are the implications for transboundary rivers?

75 FR 76987 - Disease, Disability, and Injury Prevention and Control Special Emphasis Panel (SEP...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-10

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention Disease, Disability, and Injury Prevention and Control Special Emphasis Panel (SEP): Epidemiologic and Ecologic... evaluation of applications received in response to ``Epidemiologic and Ecologic Determinants of Monkeypox in...

Soil organic matter as an important contributor to Late Quaternary sediments of the tropical West African continental margin

NASA Astrophysics Data System (ADS)

Holtvoeth, Jens; Kolonic, Sadat; Wagner, Thomas

2005-04-01

The contribution of soil organic matter (SOM) to continental margins is largely ignored in studies on the carbon budget of marine sediments. Detailed geochemical investigations of late Quaternary sediments (245-0 ka) from the Niger and Congo deep-sea fans, however, reveal that C org/N tot ratios and isotopic signatures of bulk organic matter (δ 13C org) in both fans are essentially determined by the supply of various types of SOM from the river catchments thus providing a fundamentally different interpretation of established proxies in marine sciences. On the Niger fan, increased C org/N tot and δ 13C org (up to -17‰) were driven by generally nitrogen-poor but 13C-enriched terrigenous plant debris and SOM from C 4/C 3 vegetation/Entisol domains (grass- and tree-savannah on young, sandy soils) supplied during arid climate conditions. Opposite, humid climates supported drainage of C 3/C 4 vegetation/Alfisol/Ultisol domains (forest and tree-savannah on older/developed, clay-bearing soils) that resulted in lower C org/N tot and δ 13C org (< -20‰) in the Niger fan record. Sediments from the Congo fan contain a thermally stable organic fraction that is absent on the Niger fan. This distinct organic fraction relates to strongly degraded SOM of old and highly developed, kaolinite-rich ferallitic soils (Oxisols) that cover large areas of the Congo River basin. Reduced supply of this nitrogen-rich and 12C-depleted SOM during arid climates is compensated by an elevated input of marine OM from the high-productive Congo up-welling area. This climate-driven interplay of marine productivity and fluvial SOM supply explains the significantly smaller variability and generally lower values of C org/N tot and δ 13C org for the Congo fan records. This study emphasizes that ignoring the presence of SOM results in a severe underestimation of the terrigenous organic fraction leading to erroneous paleoenvironmental interpretations at least for continental margin records. Furthermore, burial of SOM in marine sediments needs more systematic investigation combining marine and continental sciences to assess its global relevance for long-term sequestration of atmospheric CO 2.

Mapping Biomass for REDD in the Largest Forest of Central Africa: the Democratic Republic of Congo

NASA Astrophysics Data System (ADS)

Shapiro, Aurelie; Saatchi, Sassan

2014-05-01

With the support of the International Climate Initiative (ICI) of the Federal Ministry of the Environment, Conservation, and Nuclear Security, the implementation of the German Development Bank KfW, the World Wide Fund for Nature (WWF) Germany, the University of California Los Angeles (UCLA) and local DRC partners will produce a national scale biomass map for the entire forest coverage of the Democratic Republic of Congo (DRC) along with feasibility assessments of different forest protection measures within a framework of a REDD+ model project. The « Carbon Map and Model (CO2M&M) » project will produce a national forest biomass map for the DRC, which will enable quantitative assessments of carbon stocks and emissions in the largest forest of the Congo Basin. This effort will support the national REDD (Reducing Emissions from Deforestation and Degradation) program in DRC, which plays a major role in sustainable development and poverty alleviation. This map will be developed from field data, complemented by airborne LiDAR (Light Detection and Ranging) and aerial photos, systematically sampled throughout the forests of the DRC and up-scaled to satellite images to accurately estimate carbon content in all forested areas. The second component of the project is to develop specific approaches for model REDD projects in key landscapes. This project represents the largest LiDAR-derived mapping effort in Africa, under unprecedented logistical constraints, which will provide one of the poorest nations in the world with the richest airborne and satellites derived datasets for analyzing forest structure, biomass and biodiversity.

Idiosyncratic responses to climate-driven forest fragmentation and marine incursions in reed frogs from Central Africa and the Gulf of Guinea Islands.

PubMed

Bell, Rayna C; Parra, Juan L; Badjedjea, Gabriel; Barej, Michael F; Blackburn, David C; Burger, Marius; Channing, Alan; Dehling, Jonas Maximilian; Greenbaum, Eli; Gvoždík, Václav; Kielgast, Jos; Kusamba, Chifundera; Lötters, Stefan; McLaughlin, Patrick J; Nagy, Zoltán T; Rödel, Mark-Oliver; Portik, Daniel M; Stuart, Bryan L; VanDerWal, Jeremy; Zassi-Boulou, Ange Ghislain; Zamudio, Kelly R

2017-10-01

Organismal traits interact with environmental variation to mediate how species respond to shared landscapes. Thus, differences in traits related to dispersal ability or physiological tolerance may result in phylogeographic discordance among co-distributed taxa, even when they are responding to common barriers. We quantified climatic suitability and stability, and phylogeographic divergence within three reed frog species complexes across the Guineo-Congolian forests and Gulf of Guinea archipelago of Central Africa to investigate how they responded to a shared climatic and geological history. Our species-specific estimates of climatic suitability through time are consistent with temporal and spatial heterogeneity in diversification among the species complexes, indicating that differences in ecological breadth may partly explain these idiosyncratic patterns. Likewise, we demonstrated that fluctuating sea levels periodically exposed a land bridge connecting Bioko Island with the mainland Guineo-Congolian forest and that habitats across the exposed land bridge likely enabled dispersal in some species, but not in others. We did not find evidence that rivers are biogeographic barriers across any of the species complexes. Despite marked differences in the geographic extent of stable climates and temporal estimates of divergence among the species complexes, we recovered a shared pattern of intermittent climatic suitability with recent population connectivity and demographic expansion across the Congo Basin. This pattern supports the hypothesis that genetic exchange across the Congo Basin during humid periods, followed by vicariance during arid periods, has shaped regional diversity. Finally, we identified many distinct lineages among our focal taxa, some of which may reflect incipient or unrecognized species. © 2017 John Wiley & Sons Ltd.

Deflection of natural oil droplets through the water column in deep-water environments: The case of the Lower Congo Basin

NASA Astrophysics Data System (ADS)

Jatiault, Romain; Dhont, Damien; Loncke, Lies; de Madron, Xavier Durrieu; Dubucq, Dominique; Channelliere, Claire; Bourrin, François

2018-06-01

Numerous recurrent seep sites were identified in the deep-water environment of the Lower Congo Basin from the analysis of an extensive dataset of satellite-based synthetic-aperture radar images. The integration of current data was used to link natural oil slicks with active seep-related seafloor features. Acoustic Doppler current profiler measurements across the water column provided an efficient means to evaluate the horizontal deflection of oil droplets rising through the water column. Eulerian propagation model based on a range of potential ascension velocities helped to approximate the path for rising oil plume through the water column using two complementary methods. The first method consisted in simulating the reversed trajectory of oil droplets between sea-surface oil slick locations observed during current measurements and seep-related seafloor features while considering a range of ascension velocities. The second method compared the spatial spreading of natural oil slicks from 21 years of satellite monitoring observations for water depths ranging from 1200 to 2700 m against the modeled deflections during the current measurement period. The mapped oil slick origins are restricted to a 2.5 km radius circle from associated seep-related seafloor features. The two methods converge towards a range of ascension velocities for oil droplets through the water column, estimated between 3 and 8 cm s-1. The low deflection values validate that the sub-vertical projection of the average surface area of oil slicks at the sea surface can be used to identify the origin of expelled hydrocarbon from the seafloor, which expresses as specific seafloor disturbances (i.e. pockmarks or mounds) known to expel fluids.

On Intensive Late Holocene Iron Mining and Production in the Northern Congo Basin and the Environmental Consequences Associated with Metallurgy in Central Africa.

PubMed

Lupo, Karen D; Schmitt, Dave N; Kiahtipes, Christopher A; Ndanga, Jean-Paul; Young, D Craig; Simiti, Bernard

2015-01-01

An ongoing question in paleoenvironmental reconstructions of the central African rainforest concerns the role that prehistoric metallurgy played in shaping forest vegetation. Here we report evidence of intensive iron-ore mining and smelting in forested regions of the northern Congo Basin dating to the late Holocene. Volumetric estimates on extracted iron-ore and associated slag mounds from prehistoric sites in the southern Central African Republic suggest large-scale iron production on par with other archaeological and historically-known iron fabrication areas. These data document the first evidence of intensive iron mining and production spanning approximately 90 years prior to colonial occupation (circa AD 1889) and during an interval of time that is poorly represented in the archaeological record. Additional site areas pre-dating these remains by 3-4 centuries reflect an earlier period of iron production on a smaller scale. Microbotanical evidence from a sediment core collected from an adjacent riparian trap shows a reduction in shade-demanding trees in concert with an increase in light-demanding species spanning the time interval associated with iron intensification. This shift occurs during the same time interval when many portions of the Central African witnessed forest transgressions associated with a return to moister and more humid conditions beginning 500-100 years ago. Although data presented here do not demonstrate that iron smelting activities caused widespread vegetation change in Central Africa, we argue that intense mining and smelting can have localized and potentially regional impacts on vegetation communities. These data further demonstrate the high value of pairing archeological and paleoenvironmental analyses to reconstruct regional-scale forest histories.

Seismic responses and controlling factors of Miocene deepwater gravity-flow deposits in Block A, Lower Congo Basin

NASA Astrophysics Data System (ADS)

Wang, Linlin; Wang, Zhenqi; Yu, Shui; Ngia, Ngong Roger

2016-08-01

The Miocene deepwater gravity-flow sedimentary system in Block A of the southwestern part of the Lower Congo Basin was identified and interpreted using high-resolution 3-D seismic, drilling and logging data to reveal development characteristics and main controlling factors. Five types of deepwater gravity-flow sedimentary units have been identified in the Miocene section of Block A, including mass transport, deepwater channel, levee, abandoned channel and sedimentary lobe deposits. Each type of sedimentary unit has distinct external features, internal structures and lateral characteristics in seismic profiles. Mass transport deposits (MTDs) in particular correspond to chaotic low-amplitude reflections in contact with mutants on both sides. The cross section of deepwater channel deposits in the seismic profile is in U- or V-shape. The channel deposits change in ascending order from low-amplitude, poor-continuity, chaotic filling reflections at the bottom, to high-amplitude, moderate to poor continuity, chaotic or sub-parallel reflections in the middle section and to moderate-weak amplitude, good continuity, parallel or sub-parallel reflections in the upper section. The sedimentary lobes are laterally lobate, which corresponds to high-amplitude, good-continuity, moundy reflection signatures in the seismic profile. Due to sediment flux, faults, and inherited terrain, few mass transport deposits occur in the northeastern part of the study area. The front of MTDs is mainly composed of channel-levee complex deposits, while abandoned-channel and lobe-deposits are usually developed in high-curvature channel sections and the channel terminals, respectively. The distribution of deepwater channel, levee, abandoned channel and sedimentary lobe deposits is predominantly controlled by relative sea level fluctuations and to a lesser extent by tectonism and inherited terrain.

High prevalence of cannabis use among Aka foragers of the Congo Basin and its possible relationship to helminthiasis.

PubMed

Roulette, Casey J; Kazanji, Mirdad; Breurec, Sébastien; Hagen, Edward H

2016-01-01

Little is known about cannabis use in hunter-gatherers. Therefore, we investigated cannabis use in the Aka, a population of foragers of the Congo Basin. Because cannabis contains anthelminthic compounds, and the Aka have a high prevalence of helminthiasis, we also tested the hypothesis that cannabis use might be an unconscious form of self-medication against helminths. We collected self- and peer-reports of cannabis use from all adult Aka in the Lobaye district of the Central African Republic (n = 379). Because female cannabis use was low, we restricted sample collection to men. Using an immunoassay for Δ9-tetrahydrocannabinol-11-oic acid (THCA), a urinary biomarker of recent cannabis consumption, we validated cannabis use in men currently residing in camps near a logging road (n = 62). We also collected stool samples to assay worm burden. A longitudinal reinfection study was conducted among a subsample of the male participants (n = 23) who had been treated with a commercial anthelmintic 1 year ago. The prevalence of self- and peer-reported cannabis use was 70.9% among men and 6.1% among women, for a total prevalence of 38.6%. Using a 50 ng/ml threshold for THCA, 67.7% of men used cannabis. Cannabis users were significantly younger and had less material wealth than the non-cannabis users. There were significant negative associations between THCA levels and worm burden, and reinfection with helminths 1 year after treatment with a commercial anthelmintic. The prevalence of cannabis use among adult Aka men was high when compared to most global populations. THCA levels were negatively correlated with parasite infection and reinfection, supporting the self-medication hypothesis. © 2015 Wiley Periodicals, Inc.

On Intensive Late Holocene Iron Mining and Production in the Northern Congo Basin and the Environmental Consequences Associated with Metallurgy in Central Africa

PubMed Central

Lupo, Karen D.; Schmitt, Dave N.; Kiahtipes, Christopher A.; Ndanga, Jean-Paul; Young, D. Craig; Simiti, Bernard

2015-01-01

An ongoing question in paleoenvironmental reconstructions of the central African rainforest concerns the role that prehistoric metallurgy played in shaping forest vegetation. Here we report evidence of intensive iron-ore mining and smelting in forested regions of the northern Congo Basin dating to the late Holocene. Volumetric estimates on extracted iron-ore and associated slag mounds from prehistoric sites in the southern Central African Republic suggest large-scale iron production on par with other archaeological and historically-known iron fabrication areas. These data document the first evidence of intensive iron mining and production spanning approximately 90 years prior to colonial occupation (circa AD 1889) and during an interval of time that is poorly represented in the archaeological record. Additional site areas pre-dating these remains by 3-4 centuries reflect an earlier period of iron production on a smaller scale. Microbotanical evidence from a sediment core collected from an adjacent riparian trap shows a reduction in shade-demanding trees in concert with an increase in light-demanding species spanning the time interval associated with iron intensification. This shift occurs during the same time interval when many portions of the Central African witnessed forest transgressions associated with a return to moister and more humid conditions beginning 500-100 years ago. Although data presented here do not demonstrate that iron smelting activities caused widespread vegetation change in Central Africa, we argue that intense mining and smelting can have localized and potentially regional impacts on vegetation communities. These data further demonstrate the high value of pairing archeological and paleoenvironmental analyses to reconstruct regional-scale forest histories. PMID:26161540

Simulated hydrologic response to projected changes in precipitation and temperature in the Congo River basin

NASA Astrophysics Data System (ADS)

Aloysius, Noel; Saiers, James

2017-08-01

Despite their global significance, the impacts of climate change on water resources and associated ecosystem services in the Congo River basin (CRB) have been understudied. Of particular need for decision makers is the availability of spatial and temporal variability of runoff projections. Here, with the aid of a spatially explicit hydrological model forced with precipitation and temperature projections from 25 global climate models (GCMs) under two greenhouse gas emission scenarios, we explore the variability in modeled runoff in the near future (2016-2035) and mid-century (2046-2065). We find that total runoff from the CRB is projected to increase by 5 % [-9 %; 20 %] (mean - min and max - across model ensembles) over the next two decades and by 7 % [-12 %; 24 %] by mid-century. Projected changes in runoff from subwatersheds distributed within the CRB vary in magnitude and sign. Over the equatorial region and in parts of northern and southwestern CRB, most models project an overall increase in precipitation and, subsequently, runoff. A simulated decrease in precipitation leads to a decline in runoff from headwater regions located in the northeastern and southeastern CRB. Climate model selection plays an important role in future projections for both magnitude and direction of change. The multimodel ensemble approach reveals that precipitation and runoff changes under business-as-usual and avoided greenhouse gas emission scenarios (RCP8.5 vs. RCP4.5) are relatively similar in the near term but deviate in the midterm, which underscores the need for rapid action on climate change adaptation. Our assessment demonstrates the need to include uncertainties in climate model and emission scenario selection during decision-making processes related to climate change mitigation and adaptation.

Nigericin is a potent inhibitor of the early stage of vaccinia virus replication.

PubMed

Myskiw, Chad; Piper, Jessica; Huzarewich, Rhiannon; Booth, Tim F; Cao, Jingxin; He, Runtao

2010-12-01

Poxviruses remain a significant public health concern due to their potential use as bioterrorist agents and the spread of animal borne poxviruses, such as monkeypox virus, to humans. Thus, the identification of small molecule inhibitors of poxvirus replication is warranted. Vaccinia virus is the prototypic member of the Orthopoxvirus genus, which also includes variola and monkeypox virus. In this study, we demonstrate that the carboxylic ionophore nigericin is a potent inhibitor of vaccinia virus replication in several human cell lines. In HeLa cells, we found that the 50% inhibitory concentration of nigericin against vaccinia virus was 7.9 nM, with a selectivity index of 1038. We present data demonstrating that nigericin targets vaccinia virus replication at a post-entry stage. While nigericin moderately inhibits both early vaccinia gene transcription and translation, viral DNA replication and intermediate and late gene expression are severely compromised in the presence of nigericin. Our results demonstrate that nigericin has the potential to be further developed into an effective antiviral to treat poxvirus infections. Crown Copyright © 2010. Published by Elsevier B.V. All rights reserved.

Repeated trans-watershed hybridization among haplochromine cichlids (Cichlidae) was triggered by Neogene landscape evolution.

PubMed

Schwarzer, Julia; Swartz, Ernst Roelof; Vreven, Emmanuel; Snoeks, Jos; Cotterill, Fenton Peter David; Misof, Bernhard; Schliewen, Ulrich Kurt

2012-11-07

The megadiverse haplochromine cichlid radiations of the East African lakes, famous examples of explosive speciation and adaptive radiation, are according to recent studies, introgressed by different riverine lineages. This study is based on the first comprehensive mitochondrial and nuclear DNA dataset from extensive sampling of riverine haplochromine cichlids. It includes species from the lower River Congo and Angolan (River Kwanza) drainages. Reconstruction of phylogenetic hypotheses revealed the paradox of clearly discordant phylogenetic signals. Closely related mtDNA haplotypes are distributed thousands of kilometres apart and across major African watersheds, whereas some neighbouring species carry drastically divergent mtDNA haplotypes. At shallow and deep phylogenetic layers, strong signals of hybridization are attributed to the complex Late Miocene/Early Pliocene palaeohistory of African rivers. Hybridization of multiple lineages across changing watersheds shaped each of the major haplochromine radiations in lakes Tanganyika, Victoria, Malawi and the Kalahari Palaeolakes, as well as a miniature species flock in the Congo basin (River Fwa). On the basis of our results, introgression occurred not only on a spatially restricted scale, but massively over almost the whole range of the haplochromine distribution. This provides an alternative view on the origin and exceptional high diversity of this enigmatic vertebrate group.

Inorganic and organic carbon spatial variability in the Congo River during high waters (December 2013)

NASA Astrophysics Data System (ADS)

Borges, Alberto V.; Bouillon, Steven; Teodoru, Cristian; Leporcq, Bruno; Descy, Jean-Pïerre; Darchambeau, François

2014-05-01

Rivers are important components of the global carbon cycle, as they transport terrestrial organic matter from the land to the sea, and emit CO2 to the atmosphere. In particular, tropical systems that account for 60% of global freshwater discharge to the oceans. In contrast with south American rivers, very little information is available for African rivers on their carbon flows and stocks, in particular the Congo river, the second largest river in the World in terms of freshwater discharge (1457 km3 yr-1) and in terms of drainage basin (3.75 106 km2) located the second largest tropical forest in the World. Here, we report a data-set of continuous (every minute) records of the partial pressure of CO2 (pCO2) (total of 10,000 records), and discrete samples of particulate (POC) and dissolved (DOC) organic carbon (total of 75 samples) in the mainstem and major tributaries of the Congo river, along the 1700 km stretch from Kisangani to Kinshasa (total river length = 4374 km), during the high water period (December 2013). The pCO2 dynamic range was high ranging from minimum values of 2000 ppm in white waters tributaries (higher turbidity, conductivity and O2, lower DOC), up to maximal values of 18,000 ppm in blackwaters tributaries (lower turbidity, conductivity and O2, higher DOC). In the mainstem, very strong horizontal (cross-section) gradients were imposed by the presence of blackwaters close to the riverbanks and the presence of whitewaters in the middle of the river. In the mainstem, a distinct horizontal (longitudinal) pattern was observed with pCO2 increasing, and conductivity and turbidity decreasing downstream.

Spatial Patterns of Forest Cover Loss in the Democratic Republic of Congo

NASA Astrophysics Data System (ADS)

Molinario, G.; Hansen, M.; Potapov, P.; Justice, C. O.

2013-12-01

Three groups of metrics of spatial patterns of forest cover loss were calculated for the Democratic Republic of Congo (DRC). While other studies had previously assessed landscape patterns in the Congo Basin, they had done so for small areas due to data limitations. The input data for this study, the Forets d;Afrique Central Evaluee par Teledetection(FACET), allowed the analysis to be performed at the national level. FACET is a landsat-scale dataset giving an unprecedented synoptic view of forest cover and forest cover loss for the DRC for three time periods: 2000, 2005 and 2010. The three groups of metrics evaluated the following spatial characteristics of forest cover loss for the same standard 1.5km unit of area: proportions of typologies of forest lost, forest fragmentation and proximity of forest loss patches from other land cover types. Results indicate that there are several different typologies of forest cover loss in the DRC, and offer quantitative explanations of these differences, providing a valuable locally-relevant tool for land use planning, available at the national level. Spatial patterns of forest cover loss highlight differences between areas of high primary forest loss due to agriculture conversion in frontier deforestation, such as in the east of the country, areas of equivalent primary and secondary forest loss emanating from the rural complex and areas of variable proportions of primary and secondary forest loss but important ecological repercussions of forest fragmentation due to isolated, but systematic forest perforations. Typologies of spatial patterns of forest cover loss are presented as well as their correlated drivers, and ecological, conservation and land use planning considerations are discussed.

Ebola virus outbreaks in Africa: past and present.

PubMed

Muyembe-Tamfum, J J; Mulangu, S; Masumu, Justin; Kayembe, J M; Kemp, A; Paweska, Janusz T

2012-06-20

Ebola haemorrhagic fever (EHF) is a zoonosis affecting both human and non-human primates (NHP). Outbreaks in Africa occur mainly in the Congo and Nile basins. The first outbreaks of EHF occurred nearly simultaneously in 1976 in the Democratic Republic of the Congo (DRC, former Zaire) and Sudan with very high case fatality rates of 88% and 53%, respectively. The two outbreaks were caused by two distinct species of Ebola virus named Zaire ebolavirus (ZEBOV) and Sudan ebolavirus (SEBOV). The source of transmission remains unknown. After a long period of silence (1980-1993), EHF outbreaks in Africa caused by the two species erupted with increased frequency and new species were discovered, namely Côte d'Ivoire ebolavirus (CIEBOV) in 1994 in the Ivory Coast and Bundibugyo ebolavirus (BEBOV) in 2007 in Uganda. The re-emergence of EHF outbreaks in Gabon and Republic of the Congo were concomitant with an increase in mortality amongst gorillas and chimpanzees infected with ZEBOV. The human outbreaks were related to multiple, unrelated index cases who had contact with dead gorillas or chimpanzees. However, in areas where NHP were rare or absent, as in Kikwit (DRC) in 1995, Mweka (DRC) in 2007, Gulu (Uganda) in 2000 and Yambio (Sudan) in 2004, the hunting and eating of fruit bats may have resulted in the primary transmission of Ebola virus to humans. Human-to-human transmission is associated with direct contact with body fluids or tissues from an infected subject or contaminated objects. Despite several, often heroic field studies, the epidemiology and ecology of Ebola virus, including identification of its natural reservoir hosts, remains a formidable challenge for public health and scientific communities.

A Multicenter, Open-label Study of CMX001 Treatment of Serious Diseases or Conditions Caused by dsDNA Viruses

ClinicalTrials.gov

2013-08-28

Male or Female Patients With a Serious or Immediately Life-threatening; Disease or Condition Caused by CMV, ADV, HSV, VAVC, VARV or; Monkeypox Viruses(s) Who Have a Life Expectancy of ≥ 2 Weeks and for; Whom no Comparable or Satisfactory Alternative Therapy is Available

77 FR 61083 - Possession, Use, and Transfer of Select Agents and Toxins; Biennial Review

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-05

... agents or toxins in the highest tier should be further stratified based on type of use or other factors... (South American type only), Flexal virus, West African clade of Monkeypox virus, Rickettsia rickettsii... viruses have thus far produced high morbidity and mortality rates. Both Lujo and Chapare virus share other...

River mixing in the Amazon as a driver of concentration-discharge relationships

NASA Astrophysics Data System (ADS)

Moquet, Jean-Sébastien; Bouchez, Julien; Carlo Espinoza, Jhan; Martinez, Jean-Michel; Guyot, Jean-Loup; Lagane, Christelle; Filizola, Naziano; Aniceto, Keila; Noriega, Luis; Hidalgo Sanchez, Liz; Pombosa, Rodrigo; Fraizy, Pascal; Santini, William; Timouk, Franck; Vauchel, Philippe

2017-04-01

Large hydrological systems such as continental-scale river basins aggregate water from compositionally different tributaries. Here we explore how such aggregation can affect solute concentration-discharge (C-Q) relationships and thus obscure the message carried by these relationships in terms of weathering properties of the Critical Zone. We compute 10 day-frequency time series of Q and major solute (Si, Ca2+, Mg2+, K+, Na+, Cl-, SO42-) C and fluxes (F) for 13 gauging stations of the SNO-HYBAM Monitoring Program (Geodynamical, hydrological and Biogeochemical control of erosion/weathering and material transport in the Amazon, Orinoco and Congo basins) located throughout the Amazon basin, the largest river basin in the world. Concentration-discharge relationships vary in a systematic manner, shifting for most solutes from a nearly "chemostatic" behavior (constant C) at the Andean mountain front to a more "dilutional" pattern (negative C-Q relationship) towards the system mouth. Associated to this shift in trend is a shift in shape: C-Q hysteresis becomes more prominent at the most downstream stations. A simple model of tributary mixing allows us to identify the important parameters controlling C-Q trends and shapes in the mixture, and we show that for the Amazon case, the model results are in qualitative agreement with the observations. Altogether, this study suggests that mixing of water and solutes between different flowpaths leads to altered C-Q relationships.

The evolution of the River Nile. The buried saline rift lakes in Sudan—I. Bahr El Arab Rift, the Sudd buried saline lake

NASA Astrophysics Data System (ADS)

Salama, Ramsis B.

The River Nile in Sudan, was during the Tertiary, a series of closed lake basins. Each basin occupying one of the major Sudanese rift systems (Salama, 1985a). In this paper evidence is presented for the presence of the buried saline Sudd Lake in Bahr El Arab rift. The thick Tertiary sediments filling the deep grabens were eroded from the elevated blocks; Jebel Marra, Darfur Dome, Nuba Mountains and the Nile-Congo Divide. The thick carbonate deposits existing at the faulted boundaries of Bahr El Arab defines the possible boundaries between the fresh and saline water bodies. The widespread presence of kanker nodules in the sediments was a result of continuous efflorescence, leaching and evaporative processes. The highly saline zone in the central part of the Sudd was formed through the same processes with additional sulphate being added by the oxidation of the hydrogen sulphide gases emanating from the oil fields.

Gene flow between Atlantic and Pacific Ocean basins in three lineages of deep-sea clams (Bivalvia: Vesicomyidae: Pliocardiinae) and subsequent limited gene flow within the Atlantic

NASA Astrophysics Data System (ADS)

LaBella, Abigail Leavitt; Van Dover, Cindy L.; Jollivet, Didier; Cunningham, Clifford W.

2017-03-01

Pliocardiin (vesicomyid) clams rely on microbial symbionts for nutrition and are obligate inhabitants of deep-sea chemosynthetic ecosystems. Unlike many other invertebrate hosts of chemosynthetic microbes, pliocardiin clams are found in every ocean in a variety of reducing habitats, including hydrothermal vents, cold seeps, organic falls and deep-sea fans. The global distribution of pliocardiin clams suggests historical gene flow between ocean basins. We focus on 3 pliocardiin genera-'Pliocardia' I, Calyptogena and Abyssogena-each of which has a pair of sister clades in the Atlantic and Pacific. Our work tests the hypothesis that historical gene flow between the Atlantic and Pacific Oceans within these genera was interrupted by the closure of the Panamanian seaway and tests whether isolation between the ocean basins is the result of vicariance or past colonization. These questions are investigated in the context of fossil evidence, biogeography and phylogenetics. This study revealed a set of substitution rates consistent with other invertebrate studies (μ=0.8%/My/lineage), and a set consistent with much lower rates often attributed to deep-sea organisms (μ=0.3%/My/lineage). Among the Pacific/Atlantic sister pairs, 'Pliocardia' I COI divergence per lineage is intermediate (2.5%), Calyptogena is the highest (6.1%) and Abyssogena the lowest (0.8%). The substitution rates suggest that 'Pliocardia' I and Calyptogena have histories of at least 2.8 My in the Atlantic, with Calyptogena likely older. The slower rate, however, is inconsistent with both the maximum age of the family and several well studied fossils: leaving the faster rate preferred. With the faster rate, the Abyssogena southwardae clade diverged from its Pacific sister clade around 1 Mya, which likely post-dates the closure of the Isthmus of Panama and the opening of the Bering Strait. In light of this recent divergence, we test the previously proposed hypothesis that there is a high level of ongoing gene flow between Atlantic populations of A. southwardae. A. southwardae has colonized a broad geographic range of seep sites including the West Florida Escarpment, the Barbados Accretionary Prism, the Lobes of Congo, and the Mid-Atlantic Ridge north and south of the Romanche Transform Fault. Coalescent methods detect gene flow between Barbados and the Mid-Atlantic ridge; and between the West Florida Escarpment and the Lobes of Congo. All other comparisons failed to detect gene flow, contrary to prevailing interpretations of connectivity across the entire Atlantic Basin.

Hedgehog Zoonoses

PubMed Central

Riley, Patricia Y.

2005-01-01

Exotic pets, including hedgehogs, have become popular in recent years among pet owners, especially in North America. Such animals can carry and introduce zoonotic agents, a fact well illustrated by the recent outbreak of monkeypox in pet prairie dogs. We reviewed known and potential zoonotic diseases that could be carried and transmitted by pet hedgehogs or when rescuing and caring for wild-caught hedgehogs. PMID:15705314

Efficacy of CMX001 as a Prophylactic and Presymptomatic Antiviral Agent in New Zealand White Rabbits Infected with Rabbitpox Virus, a Model for Orthopoxvirus Infections of Humans

PubMed Central

Rice, Amanda D.; Adams, Mathew M.; Lampert, Bernhard; Foster, Scott; Lanier, Randall; Robertson, Alice; Painter, George; Moyer, Richard W.

2011-01-01

CMX001, a lipophilic nucleotide analog formed by covalently linking 3-(hexdecyloxy)propan-1-ol to cidofovir (CDV), is being developed as a treatment for smallpox. CMX001 has dramatically increased potency versus CDV against all dsDNA viruses and, in contrast to CDV, is orally available and has shown no evidence of nephrotoxicity in healthy volunteers or severely ill transplant patients to date. Although smallpox has been eliminated from the environment, treatments are urgently being sought due to the risk of smallpox being used as a bioterrorism agent and for monkeypox virus, a zoonotic disease of Africa, and adverse reactions to smallpox virus vaccinations. In the absence of human cases of smallpox, new treatments must be tested for efficacy in animal models. Here we first review and discuss the rabbitpox virus (RPV) infection of New Zealand White rabbits as a model for smallpox to test the efficacy of CMX001 as a prophylactic and early disease antiviral. Our results should also be applicable to monkeypox virus infections and for treatment of adverse reactions to smallpox vaccination. PMID:21369346

The conchostracan subgenus Orthestheria (Migransia) from the Tacuarembó Formation (Late Jurassic-?Early Cretaceous, Uruguay) with notes on its geological age

NASA Astrophysics Data System (ADS)

Yanbin, Shen; Gallego, Oscar F.; Martínez, Sergio

2004-04-01

Conchostracans from the Tacuarembó Formation s.s. of Uruguay are reassigned to the subgenus Orthestheria (Migransia) Chen and Shen. They show more similarities to genera of Late Jurassic age in the Congo Basin and China than to those of Early Cretaceous age. On the basis of the character of the conchostracans, we suggest that the Tacuarembó Formation is unlikely to be older than Late Jurassic. It is probably Kimmeridgian, but an Early Cretaceous age cannot be excluded. This finding is consistent with isotopic dating of the overlying basalts, as well as the age range of recently described fossil freshwater sharks.

Understanding the degradation of Congo red and bacterial diversity in an air-cathode microbial fuel cell being evaluated for simultaneous azo dye removal from wastewater and bioelectricity generation.

PubMed

Sun, Jian; Li, Youming; Hu, Yongyou; Hou, Bin; Zhang, Yaping; Li, Sizhe

2013-04-01

We investigated the mechanism of Congo red degradation and bacterial diversity in a single-chambered microbial fuel cell (MFC) incorporating a microfiltration membrane and air-cathode. The MFC was operated continuously for more than 4 months using a mixture of Congo red and glucose as fuel. We demonstrated that the Congo red azo bonds were reduced at the anode to form aromatic amines. This is consistent with the known mechanism of anaerobic biodegradation of azo dyes. The MFC developed a less dense biofilm at the anode in the presence of Congo red compared to its absence indicating that Congo red degradation negatively affected biofilm formation. Denaturing gradient gel electrophoresis and direct 16S ribosomal DNA gene nucleotide sequencing revealed that the microbial communities differed depending on whether Congo red was present in the MFC. Geobacter-like species known to generate electricity were detected in the presence or absence of Congo red. In contrast, Azospirillum, Methylobacterium, Rhodobacter, Desulfovibrio, Trichococcus, and Bacteroides species were only detected in its presence. These species were most likely responsible for degrading Congo red.

An initial investigation into the organic matter biogeochemistry of the Congo River

USGS Publications Warehouse

Spencer, Robert G.M.; Hernes, Peter J.; Aufdenkampe, Anthony K.; Baker, Andy; Gulliver, Pauline; Stubbins, Aron; Aiken, George R.; Dyda, Rachael Y.; Butler, Kenna D.; Mwamba, Vincent L.; Mangangu, Arthur M.; Wabakanghanzi, Jose N.; Six, Johan

2012-01-01

The Congo River, which drains pristine tropical forest and savannah and is the second largest exporter of terrestrial carbon to the ocean, was sampled in early 2008 to investigate organic matter (OM) dynamics in this historically understudied river basin. We examined the elemental (%OC, %N, C:N), isotopic (δ13C, Δ14C, δ15N) and biochemical composition (lignin phenols) of coarse particulate (>63 μm; CPOM) and fine particulate (0.7–63 μm; FPOM) OM and DOC, δ13C, Δ14C and lignin phenol composition with respect to dissolved OM (14C = -62.2 ± -13.2‰, n = 5) compared to CPOM and DOM (mean Δ14C = 55.7 ± 30.6‰, n = 4 and 73.4 ± 16.1‰, n = 5 respectively). The modern radiocarbon ages for DOM belie a degraded lignin compositional signature (i.e. elevated acid:aldehyde ratios (Ad:Al) relative to CPOM and FPOM), and indicate that the application of OM degradation patterns derived from particulate phase studies to dissolved samples needs to be reassessed: these elevated ratios are likely attributable to fractionation processes during solubilization of plant material. The relatively low DOM carbon-normalized lignin yields (Λ8; 0.67–1.12 (mg(100 mg OC)-1)) could also reflect fractionation processes, however, they have also been interpreted as an indication of significant microbial or algal sources of DOM. CPOM appears to be well preserved higher vascular plant material as evidenced by its modern radiocarbon age, elevated C:N (17.2–27.1) and Λ8 values (4.56–7.59 (mg(100 mg OC)-1)). In relation to CPOM, the aged FPOM fraction (320–580 ybp 14C ages) was comparatively degraded, as demonstrated by its nitrogen enrichment (C:N 11.4–14.3), lower Λ8 (2.80–4.31 (mg(100 mg OC)-1)) and elevated lignin Ad:Al values similar to soil derived OM. In this study we observed little modification of the OM signature from sample sites near the cities of Brazzaville and Kinshasa to the head of the estuary (~350 km) highlighting the potential for future studies to assess seasonal and long-term OM dynamics from this logistically feasible location and derive relevant information with respect to OM exported to the Atlantic Ocean. The relative lack of OM data for the Congo River Basin highlights the importance of studies such as this for establishing baselines upon which to gauge future change.

76 FR 76219 - Designation of Additional Individual Pursuant to Executive Order 13413

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-06

... Democratic Republic of Congo.'' DATES: The designation by the Director of OFAC of the individual identified... the Congo constitutes and unusual and extraordinary threat to the foreign policy of the United States... Ntaberi; DOB 4 Apr 1976; POB Walikale Territory, Democratic Republic of the Congo; nationality Congo...

75 FR 79443 - Unblocking of Specially Designated National Pursuant to Executive Order 13413

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-20

... the Democratic Republic of Congo'' and who has been removed from OFAC's list of Specially Designated... imposed sanctions relating to the situation in the Democratic Republic of the Congo. Section 1 of the... Congo, Democratic Republic of the; Passport C0323172 (Congo, Democratic Republic of the) (individual...

Department of Defense Chemical and Biological Defense Program. Volume 1: Annual Report to Congress

DTIC Science & Technology

2003-04-01

Albuquerque Operations Office at Kirtland AFB, New Mexico , conducts a Radiological Emergency Team Operations Course; Radiological Emer- gency Medical...Nevada, and Kirtland Air Force Base, New Mexico . • MARFORPAC sponsored a force protection initiative funded by DTRA. DTRA will conduct an independent...strains and isolates from camelpox, cowpox, ectromelia, gerbilpox, Herpes, monkeypox, myxoma, rabbitpox, raccoonpox, skunkpox, vaccinia and varicella

Smallpox virus resequencing GeneChips can also rapidly ascertain species status for some zoonotic non-variola orthopoxviruses.

PubMed

Sulaiman, Irshad M; Sammons, Scott A; Wohlhueter, Robert M

2008-04-01

We recently developed a set of seven resequencing GeneChips for the rapid sequencing of Variola virus strains in the WHO Repository of the Centers for Disease Control and Prevention. In this study, we attempted to hybridize these GeneChips with some known non-Variola orthopoxvirus isolates, including monkeypox, cowpox, and vaccinia viruses, for rapid detection.

Nonhuman Primates are Protected from Smallpox Virus or Monkeypox Virus Challenges by the Antiviral Drug ST-246

DTIC Science & Technology

2009-06-01

Drug ST-246 John Huggins,1 Arthur Goff,1 Lisa Hensley,1 Eric Mucker,1 Josh Shamblin,1 Carly Wlazlowski,1 Wendy Johnson,1 Jennifer Chapman,1 Tom Larsen...Hauer, M. Layton , J. McDade, M. T. Osterholm, T. O’Toole, G. Parker, T. Perl, P. K. Russell, K. Tonat, and the Working Group on Civilian Biodefense

Congo red modulates ACh-induced Ca2+ oscillations in single pancreatic acinar cells of mice

PubMed Central

Huang, Ze-bing; Wang, Hai-yan; Sun, Na-na; Wang, Jing-ke; Zhao, Meng-qin; Shen, Jian-xin; Gao, Ming; Hammer, Ronald P; Fan, Xue-gong; Wu, Jie

2014-01-01

Aim: Congo red, a secondary diazo dye, is usually used as an indicator for the presence of amyloid fibrils. Recent studies show that congo red exerts neuroprotective effects in a variety of models of neurodegenerative diseases. However, its pharmacological profile remains unknown. In this study, we investigated the effects of congo red on ACh-induced Ca2+ oscillations in mouse pancreatic acinar cells in vitro. Methods: Acutely dissociated pancreatic acinar cells of mice were prepared. A U-tube drug application system was used to deliver drugs into the bath. Intracellular Ca2+ oscillations were monitored by whole-cell recording of Ca2+-activated Cl− currents and by using confocal Ca2+ imaging. For intracellular drug application, the drug was added in pipette solution and diffused into cell after the whole-cell configuration was established. Results: Bath application of ACh (10 nmol/L) induced typical Ca2+ oscillations in dissociated pancreatic acinar cells. Addition of congo red (1, 10, 100 μmol/L) dose-dependently enhanced Ach-induced Ca2+ oscillations, but congo red alone did not induce any detectable response. Furthermore, this enhancement depended on the concentrations of ACh: congo red markedly enhanced the Ca2+ oscillations induced by ACh (10–30 nmol/L), but did not alter the Ca2+ oscillations induced by ACh (100–10000 nmol/L). Congo red also enhanced the Ca2+ oscillations induced by bath application of IP3 (30 μmol/L). Intracellular application of congo red failed to alter ACh-induced Ca2+ oscillations. Conclusion: Congo red significantly modulates intracellular Ca2+ signaling in pancreatic acinar cells, and this pharmacological effect should be fully considered when developing congo red as a novel therapeutic drug. PMID:25345744

Co-administration of the broad-spectrum antiviral, brincidofovir (CMX001), with smallpox vaccine does not compromise vaccine protection in mice challenged with ectromelia virus.

PubMed

Parker, Scott; Crump, Ryan; Foster, Scott; Hartzler, Hollyce; Hembrador, Ed; Lanier, E Randall; Painter, George; Schriewer, Jill; Trost, Lawrence C; Buller, R Mark

2014-11-01

Natural orthopoxvirus outbreaks such as vaccinia, cowpox, cattlepox and buffalopox continue to cause morbidity in the human population. Monkeypox virus remains a significant agent of morbidity and mortality in Africa. Furthermore, monkeypox virus's broad host-range and expanding environs make it of particular concern as an emerging human pathogen. Monkeypox virus and variola virus (the etiological agent of smallpox) are both potential agents of bioterrorism. The first line response to orthopoxvirus disease is through vaccination with first-generation and second-generation vaccines, such as Dryvax and ACAM2000. Although these vaccines provide excellent protection, their widespread use is impeded by the high level of adverse events associated with vaccination using live, attenuated virus. It is possible that vaccines could be used in combination with antiviral drugs to reduce the incidence and severity of vaccine-associated adverse events, or as a preventive in individuals with uncertain exposure status or contraindication to vaccination. We have used the intranasal mousepox (ectromelia) model to evaluate the efficacy of vaccination with Dryvax or ACAM2000 in conjunction with treatment using the broad spectrum antiviral, brincidofovir (BCV, CMX001). We found that co-treatment with BCV reduced the severity of vaccination-associated lesion development. Although the immune response to vaccination was quantifiably attenuated, vaccination combined with BCV treatment did not alter the development of full protective immunity, even when administered two days following ectromelia challenge. Studies with a non-replicating vaccine, ACAM3000 (MVA), confirmed that BCV's mechanism of attenuating the immune response following vaccination with live virus was, as expected, by limiting viral replication and not through inhibition of the immune system. These studies suggest that, in the setting of post-exposure prophylaxis, co-administration of BCV with vaccination should be considered a first response to a smallpox emergency in subjects of uncertain exposure status or as a means of reduction of the incidence and severity of vaccine-associated adverse events. Copyright © 2014 Elsevier B.V. All rights reserved.

A refined model of sedimentary rock cover in the southeastern part of the Congo basin from GOCE gravity and vertical gravity gradient observations

NASA Astrophysics Data System (ADS)

Martinec, Zdeněk; Fullea, Javier

2015-03-01

We aim to interpret the vertical gravity and vertical gravity gradient of the GOCE-GRACE combined gravity model over the southeastern part of the Congo basin to refine the published model of sedimentary rock cover. We use the GOCO03S gravity model and evaluate its spherical harmonic representation at or near the Earth's surface. In this case, the gradiometry signals are enhanced as compared to the original measured GOCE gradients at satellite height and better emphasize the spatial pattern of sedimentary geology. To avoid aliasing, the omission error of the modelled gravity induced by the sedimentary rocks is adjusted to that of the GOCO03S gravity model. The mass-density Green's functions derived for the a priori structure of the sediments show a slightly greater sensitivity to the GOCO03S vertical gravity gradient than to the vertical gravity. Hence, the refinement of the sedimentary model is carried out for the vertical gravity gradient over the basin, such that a few anomalous values of the GOCO03S-derived vertical gravity gradient are adjusted by refining the model. We apply the 5-parameter Helmert's transformation, defined by 2 translations, 1 rotation and 2 scale parameters that are searched for by the steepest descent method. The refined sedimentary model is only slightly changed with respect to the original map, but it significantly improves the fit of the vertical gravity and vertical gravity gradient over the basin. However, there are still spatial features in the gravity and gradiometric data that remain unfitted by the refined model. These may be due to lateral density variation that is not contained in the model, a density contrast at the Moho discontinuity, lithospheric density stratifications or mantle convection. In a second step, the refined sedimentary model is used to find the vertical density stratification of sedimentary rocks. Although the gravity data can be interpreted by a constant sedimentary density, such a model does not correspond to the gravitational compaction of sedimentary rocks. Therefore, the density model is extended by including a linear increase in density with depth. Subsequent L2 and L∞ norm minimization procedures are applied to find the density parameters by adjusting both the vertical gravity and the vertical gravity gradient. We found that including the vertical gravity gradient in the interpretation of the GOCO03S-derived data reduces the non-uniqueness of the inverse gradiometric problem for density determination. The density structure of the sedimentary formations that provide the optimum predictions of the GOCO03S-derived gravity and vertical gradient of gravity consists of a surface density contrast with respect to surrounding rocks of 0.24-0.28 g/cm3 and its decrease with depth of 0.05-0.25 g/cm3 per 10 km. Moreover, the case where the sedimentary rocks are gravitationally completely compacted in the deepest parts of the basin is supported by L∞ norm minimization. However, this minimization also allows a remaining density contrast at the deepest parts of the sedimentary basin of about 0.1 g/cm3.

Comparative phylogeography of African fruit bats (Chiroptera, Pteropodidae) provide new insights into the outbreak of Ebola virus disease in West Africa, 2014-2016.

PubMed

Hassanin, Alexandre; Nesi, Nicolas; Marin, Julie; Kadjo, Blaise; Pourrut, Xavier; Leroy, Éric; Gembu, Guy-Crispin; Musaba Akawa, Prescott; Ngoagouni, Carine; Nakouné, Emmanuel; Ruedi, Manuel; Tshikung, Didier; Pongombo Shongo, Célestin; Bonillo, Céline

Both Ebolavirus and Marburgvirus were detected in several fruit bat species of the family Pteropodidae, suggesting that this taxon plays a key role in the life cycle of filoviruses. After four decades of Zaire Ebolavirus (ZEBOV) outbreaks in Central Africa, the virus was detected for the first time in West Africa in 2014. To better understand the role of fruit bats as potential reservoirs and circulating hosts between Central and West Africa, we examine here the phylogeny and comparative phylogeography of Pteropodidae. Our phylogenetic results confirm the existence of four independent lineages of African fruit bats: the genera Eidolon and Rousettus, and the tribes Epomophorini and Scotonycterini, and indicate that the three species suspected to represent ZEBOV reservoir hosts (Epomops franqueti, Hypsignathus monstrosus, and Myonycteris torquata) belong to an African clade that diversified rapidly around 8-7 Mya. To test for phylogeographic structure and for recent gene flow from Central to West Africa, we analysed the nucleotide variation of 675 cytochrome b gene (Cytb) sequences, representing eight fruit bat species collected in 48 geographic localities. Within Epomophorina, our mitochondrial data do not support the monophyly of two genera (Epomops and Epomophorus) and four species (Epomophorus gambianus, Epomops franqueti, Epomops buettikoferi, and Micropteropus pusillus). In Epomops, however, we found two geographic haplogroups corresponding to the Congo Basin and Upper Guinea forests, respectively. By contrast, we found no genetic differentiation between Central and West African populations for all species known to make seasonal movements, Eidolon helvum, E. gambianus, H. monstrosus, M. pusillus, Nanonycteris veldkampii, and Rousettus aegyptiacus. Our results suggest that only three fruit bat species were able to disperse directly ZEBOV from the Congo Basin to Upper Guinea: E. helvum, H. monstrosus, and R. aegyptiacus. Copyright © 2016 Académie des sciences. Published by Elsevier SAS. All rights reserved.

Aboveground vs. Belowground Carbon Stocks in African Tropical Lowland Rainforest: Drivers and Implications.

PubMed

Doetterl, Sebastian; Kearsley, Elizabeth; Bauters, Marijn; Hufkens, Koen; Lisingo, Janvier; Baert, Geert; Verbeeck, Hans; Boeckx, Pascal

2015-01-01

African tropical rainforests are one of the most important hotspots to look for changes in the upcoming decades when it comes to C storage and release. The focus of studying C dynamics in these systems lies traditionally on living aboveground biomass. Belowground soil organic carbon stocks have received little attention and estimates of the size, controls and distribution of soil organic carbon stocks are highly uncertain. In our study on lowland rainforest in the central Congo basin, we combine both an assessment of the aboveground C stock with an assessment of the belowground C stock and analyze the latter in terms of functional pools and controlling factors. Our study shows that despite similar vegetation, soil and climatic conditions, soil organic carbon stocks in an area with greater tree height (= larger aboveground carbon stock) were only half compared to an area with lower tree height (= smaller aboveground carbon stock). This suggests that substantial variability in the aboveground vs. belowground C allocation strategy and/or C turnover in two similar tropical forest systems can lead to significant differences in total soil organic C content and C fractions with important consequences for the assessment of the total C stock of the system. We suggest nutrient limitation, especially potassium, as the driver for aboveground versus belowground C allocation. However, other drivers such as C turnover, tree functional traits or demographic considerations cannot be excluded. We argue that large and unaccounted variability in C stocks is to be expected in African tropical rain-forests. Currently, these differences in aboveground and belowground C stocks are not adequately verified and implemented mechanistically into Earth System Models. This will, hence, introduce additional uncertainty to models and predictions of the response of C storage of the Congo basin forest to climate change and its contribution to the terrestrial C budget.

Aboveground vs. Belowground Carbon Stocks in African Tropical Lowland Rainforest: Drivers and Implications

PubMed Central

Bauters, Marijn; Hufkens, Koen; Lisingo, Janvier; Baert, Geert; Verbeeck, Hans; Boeckx, Pascal

2015-01-01

Background African tropical rainforests are one of the most important hotspots to look for changes in the upcoming decades when it comes to C storage and release. The focus of studying C dynamics in these systems lies traditionally on living aboveground biomass. Belowground soil organic carbon stocks have received little attention and estimates of the size, controls and distribution of soil organic carbon stocks are highly uncertain. In our study on lowland rainforest in the central Congo basin, we combine both an assessment of the aboveground C stock with an assessment of the belowground C stock and analyze the latter in terms of functional pools and controlling factors. Principal Findings Our study shows that despite similar vegetation, soil and climatic conditions, soil organic carbon stocks in an area with greater tree height (= larger aboveground carbon stock) were only half compared to an area with lower tree height (= smaller aboveground carbon stock). This suggests that substantial variability in the aboveground vs. belowground C allocation strategy and/or C turnover in two similar tropical forest systems can lead to significant differences in total soil organic C content and C fractions with important consequences for the assessment of the total C stock of the system. Conclusions/Significance We suggest nutrient limitation, especially potassium, as the driver for aboveground versus belowground C allocation. However, other drivers such as C turnover, tree functional traits or demographic considerations cannot be excluded. We argue that large and unaccounted variability in C stocks is to be expected in African tropical rain-forests. Currently, these differences in aboveground and belowground C stocks are not adequately verified and implemented mechanistically into Earth System Models. This will, hence, introduce additional uncertainty to models and predictions of the response of C storage of the Congo basin forest to climate change and its contribution to the terrestrial C budget. PMID:26599231

The silicon isotopic composition of fine-grained river sediments and its relation to climate and lithology

NASA Astrophysics Data System (ADS)

Bayon, G.; Delvigne, C.; Ponzevera, E.; Borges, A. V.; Darchambeau, F.; De Deckker, P.; Lambert, T.; Monin, L.; Toucanne, S.; André, L.

2018-05-01

The δ30Si stable isotopic composition of silicon in soils and fine-grained sediments can provide insights into weathering processes on continents, with important implications on the Si budget of modern and past oceans. To further constrain the factors controlling the distribution of Si isotopes in sediments, we have analysed a large number (n = 50) of separate size-fractions of sediments and suspended particulate materials collected near the mouth of rivers worldwide. This includes some of the world's largest rivers (e.g. Amazon, Congo, Mackenzie, Mississippi, Murray-Darling, Nile, Yangtze) and rivers from the case study areas of the Congo River Basin and Northern Ireland. Silt-size fractions exhibit a mean Si isotopic composition (δ30Si = -0.21 ± 0.19‰; 2 s.d.) similar to that previously inferred for the upper continental crust. In contrast, clay-size fractions display a much larger range of δ30Si values from -0.11‰ to -2.16‰, which yield a global δ30Siclay of -0.57 ± 0.60‰ (2 s.d.) representative of the mean composition of the average weathered continental crust. Overall, these new data show that the Si isotopic signature transported by river clays is controlled by the degree of chemical weathering, as inferred from strong relationships with Al/Si ratios. At a global scale, the clay-bound Si isotopic composition of the world's largest river systems demonstrates a link with climate, defining a general correlation with mean annual temperature (MAT) in corresponding drainage basins. While the distribution of Si isotopes in river sediments also appears to be influenced by the tectonic setting, lithological effects and sediment recycling from former sedimentary cycles, our results pave the way for their use as paleo-weathering and paleo-climate proxies in the sedimentary record.

Restoring the supercontinent Columbia and tracing its fragments after its breakup: A new configuration and a Super-Horde hypothesis

NASA Astrophysics Data System (ADS)

Yakubchuk, Alexander

2010-09-01

Paleoproterozoic collisional (internal) and accretionary (external) orogens, additionally constrained by the matches between the Archaean granulite-gneiss and granite-greenstone terranes, are used to reconstruct the Mesoproterozoic supercontinent Columbia. The Archaean granulite-gneiss terranes occupy an axial position, forming the Archaean Super-Horde, traceable through almost all present cratons. Restored Columbia is a 30,000 km long supercontinent, assembled by ca 1.85 Ga. There is no evidence of its breakup during the Mesoproterozoic, and it subsequently grew via external accretion until ca. 1.25 Ga. After 1.25 Ga, the Atlantica group of cratons was split from Columbia and rotated to collide with the remaining intact part of Columbia to produce the 1.0 Ga Grenville orogen, hence assembling the supercontinent Rodinia. At 1000-720 Ma, penetration of oceanic spreading centres into Rodinia between Siberia and the Australian cratons split the remaining part of Columbia into the Ur and Nena cratonic groups. Nena was then quickly rifted apart into Laurentia, Eastern Europe, and Siberia. Siberia started its drift from the present western edge of Laurentia towards Eastern Europe. This drift might have caused the separation from Nena of parts of the Palaeoproterozoic external orogen to form the Great Steppe superterrane, which later was assimilated into the basement of Neoproterozoic to Palaeozoic magmatic arcs with adjacent backarc oceanic basins, whose fragments are at present found inside the Central Asia supercollage. Simultaneously with Siberia, the remaining intact Ur began moving in the opposite direction around Atlantica. During this translation, Atlantica was fragmented into Congo-Tanzania, West Africa, Amazonia and Rio-de-la-Plata with opening of the internal Brasiliano oceanic basin and its subsequent suturing. This closure might have happened due to the arrival of Ur, whose Kalahari and India portions collided with Congo-Tanzania to produce the Damara and Mozambique orogens, welding Ur and Atlantica into Gondwana at 540-500 Ma.

Skills of General Circulation and Earth System Models in reproducing streamflow to the ocean: the case of Congo river

NASA Astrophysics Data System (ADS)

Santini, M.; Caporaso, L.

2017-12-01

Although the importance of water resources in the context of climate change, it is still difficult to correctly simulate the freshwater cycle over the land via General Circulation and Earth System Models (GCMs and ESMs). Existing efforts from the Climate Model Intercomparison Project 5 (CMIP5) were mainly devoted to the validation of atmospheric variables like temperature and precipitation, with low attention to discharge.Here we investigate the present-day performances of GCMs and ESMs participating to CMIP5 in simulating the discharge of the river Congo to the sea thanks to: i) the long-term availability of discharge data for the Kinshasa hydrological station representative of more than 95% of the water flowing in the whole catchment; and ii) the River's still low influence by human intervention, which enables comparison with the (mostly) natural streamflow simulated within CMIP5.Our findings suggest how most of models appear overestimating the streamflow in terms of seasonal cycle, especially in the late winter and spring, while overestimation and variability across models are lower in late summer. Weighted ensemble means are also calculated, based on simulations' performances given by several metrics, showing some improvements of results.Although simulated inter-monthly and inter-annual percent anomalies do not appear significantly different from those in observed data, when translated into well consolidated indicators of drought attributes (frequency, magnitude, timing, duration), usually adopted for more immediate communication to stakeholders and decision makers, such anomalies can be misleading.These inconsistencies produce incorrect assessments towards water management planning and infrastructures (e.g. dams or irrigated areas), especially if models are used instead of measurements, as in case of ungauged basins or for basins with insufficient data, as well as when relying on models for future estimates without a preliminary quantification of model biases.

Cultural variation in the use of overimitation by the Aka and Ngandu of the Congo Basin.

PubMed

Berl, Richard E W; Hewlett, Barry S

2015-01-01

Studies in Western cultures have observed that both children and adults tend to overimitate, copying causally irrelevant actions in the presence of clear causal information. Investigation of this feature in non-Western groups has found little difference cross-culturally in the frequency or manner with which individuals overimitate. However, each of the non-Western populations studied thus far has a history of close interaction with Western cultures, such that they are now far removed from life in a hunter-gatherer or other small-scale culture. To investigate overimitation in a context of limited Western cultural influences, we conducted a study with the Aka hunter-gatherers and neighboring Ngandu horticulturalists of the Congo Basin rainforest in the southern Central African Republic. Aka children, Ngandu children, and Aka adults were presented with a reward retrieval task similar to those performed in previous studies, involving a demonstrated sequence of causally relevant and irrelevant actions. Aka children were found not to overimitate as expected, instead displaying one of the lowest rates of overimitation seen under similar conditions. Aka children copied fewer irrelevant actions than Aka adults, used a lower proportion of irrelevant actions than Ngandu children and Aka adults, and had less copying fidelity than Aka adults. Measures from Ngandu children were intermediate between the two Aka groups. Of the participants that succeeded in retrieving the reward, 60% of Aka children used emulation rather than imitation, compared to 15% of Ngandu children, 11% of Aka adults, and 0% of Western children of similar age. From these results, we conclude that cross-cultural variation exists in the use of overimitation during childhood. Further study is needed under a more diverse representation of cultural and socioeconomic groups in order to investigate the cognitive underpinnings of overimitation and its possible influences on social learning and the biological and cultural evolution of our species.

Cold-seep-like macrofaunal communities in organic- and sulfide-rich sediments of the Congo deep-sea fan

NASA Astrophysics Data System (ADS)

Olu, K.; Decker, C.; Pastor, L.; Caprais, J.-C.; Khripounoff, A.; Morineaux, M.; Ain Baziz, M.; Menot, L.; Rabouille, C.

2017-08-01

Methane-rich fluids arising from organic matter diagenesis in deep sediment layers sustain chemosynthesis-based ecosystems along continental margins. This type of cold seep develops on pockmarks along the Congo margin, where fluids migrate from deep-buried paleo-channels of the Congo River, acting as reservoirs. Similar ecosystems based on shallow methane production occur in the terminal lobes of the present-day Congo deep-sea fan, which is supplied by huge quantities of primarily terrestrial material carried by turbiditic currents along the 800 km channel, and deposited at depths of up to nearly 5000 m. In this paper, we explore the effect of this carbon enrichment of deep-sea sediments on benthic macrofauna, along the prograding lobes fed by the current active channel, and on older lobes receiving less turbiditic inputs. Macrofaunal communities were sampled using either USNEL cores on the channel levees, or ROV blade cores in the chemosynthesis-based habitats patchily distributed in the active lobe complex. The exceptionally high organic content of the surface sediment in the active lobe complex was correlated with unusual densities of macrofauna for this depth, enhanced by a factor 7-8, compared with those of the older, abandoned lobe, whose sediment carbon content is still higher than in Angola Basin at same depth. Macrofaunal communities, dominated by cossurid polychaetes and tanaids were also more closely related to those colonizing low-flow cold seeps than those of typical deep-sea sediment. In reduced sediments, microbial mats and vesicomyid bivalve beds displayed macrofaunal community patterns that were similar to their cold-seep counterparts, with high densities, low diversity and dominance of sulfide-tolerant polychaetes and gastropods in the most sulfidic habitats. In addition, diversity was higher in vesicomyid bivalve beds, which appeared to bio-irrigate the upper sediment layers. High beta-diversity is underscored by the variability of geochemical gradients in vesicomyid assemblages, and by the vesicomyid population characteristics that vary in density, size and composition. By modifying the sediment geochemistry differently according to their morphology and physiology, the different vesicomyid species play an important role structuring macrofauna composition and vertical distribution. Dynamics of turbiditic deposits at a longer temporal scale (thousands of years) and their spatial distribution in the lobe area also resulted in high heterogeneity of the "cold-seep-like communities". Dynamics of chemosynthetic habitats and associated macrofauna in the active lobe area resembled those previously observed at the Regab pockmark along the Congo margin and rapid succession is expected to cope with high physical disturbance by frequent turbiditic events and huge sedimentation rates. Finally, we propose a model of the temporal evolution of these peculiar habitats and communities on longer timescales in response to changes in distributary channels within the lobe complex.

Molecular phylogenetics reveals convergent evolution in lower Congo River spiny eels.

PubMed

Alter, S Elizabeth; Brown, Bianca; Stiassny, Melanie L J

2015-10-15

The lower Congo River (LCR) is a region of exceptional species diversity and endemism in the Congo basin, including numerous species of spiny eels (genus Mastacembelus). Four of these exhibit distinctive phenotypes characterized by greatly reduced optic globes deeply embedded into the head (cryptophthalmia) and reduced (or absent) melanin pigmentation, among other characteristics. A strikingly similar cryptophthalmic phenotype is also found in members of a number of unrelated fish families, strongly suggesting the possibility of convergent evolution. However, little is known about the evolutionary processes that shaped diversification in LCR Mastacembelus, their biogeographic origins, or when colonization of the LCR occurred. We sequenced mitochondrial and nuclear genes from Mastacembelus species collected in the lower Congo River, and compared them with other African species and Asian representatives as outgroups. We analyzed the sequence data using Maximum Likelihood and Bayesian phylogenetic inference. Bayesian and Maximum Likelihood phylogenetic analyses, and Bayesian coalescent methods for species tree reconstruction, reveal that endemic LCR spiny eels derive from two independent origins, clearly demonstrating convergent evolution of the cryptophthalmic phenotype. Mastacembelus crassus, M. aviceps, and M. simbi form a clade, allied to species found in southern, eastern and central Africa. Unexpectedly, M. brichardi and brachyrhinus fall within a clade otherwise endemic to Lake Tanganikya (LT) ca. 1500 km east of the LCR. Divergence dating suggests the ages of these two clades of LCR endemics differ markedly. The age of the crassus group is estimated at ~4 Myr while colonization of the LCR by the brichardi-brachyrhinus progenitor was considerably more recent, dated at ~0.5 Myr. The phylogenetic framework of spiny eels presented here, the first to include LCR species, demonstrates that cryptophthalmia and associated traits evolved at least twice in Mastacembelus: once in M. brichardi and at least once in the M. crassus clade. Timing of diversification is broadly consistent with the onset of modern high-energy flow conditions in the LCR and with previous studies of endemic cichlids. The close genetic relationship between M. brichardi and M. brachyrhinus is particularly notable given the extreme difference in phenotype between these species, and additional work is needed to better understand the evolutionary history of diversification in this clade. The findings presented here demonstrate strong, multi-trait convergence in LCR spiny eels, suggesting that extreme selective pressures have shaped numerous phenotypic attributes of the endemic species of this region.

Acute Arthritis in Crimean-Congo Hemorrhagic Fever

PubMed Central

Ahmeti, Salih; Ajazaj-Berisha, Lindita; Halili, Bahrije; Shala, Anita

2014-01-01

Crimean-Congo hemorrhagic fever is a severe viral disease caused by a Nairovirus. An atypical manifestation in the form of acute arthritis was found in a confirmed Crimean-Congo hemorrhagic fever virus Kosova-Hoti strain positive patient. Acute arthritis in Crimean-Congo hemorrhagic fever (CCHF) may be as a result of immune mechanisms or the bleeding disorder underlying CCHF. PMID:24926169

Crimean-Congo Hemorrhagic Fever Virus in Pakistan.

PubMed

Ijaz, Muhammad; Rahim, Afaq; Ali, Iftikhar

2017-01-01

The Crimean-Congo hemorrhagic fever is a zoonotic disease transmitted by ticks and is characterized by fever and bleeding. It was seen for the first time in the south of present day Ukraine and thus named, Crimean fever. 1 In 1956, the virus was isolated in a patient with similar symptoms residing in Congo, Kenya and the virus was named Congo virus. The viruses causing these two diseases were the same and hence was termed Crimean-Congo hemorrhagic fever virus (CCHFV). Humans are the only known host that develops disease. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

Diversity, Replication, Pathogenicity and Cell Biology of Crimean Congo Hemorrhagic Fever Virus

DTIC Science & Technology

2007-10-01

Crimean Congo Hemorrhagic Fever Virus PRINCIPAL INVESTIGATOR: Adolfo García-Sastre, Ph.D. CONTRACTING...Diversity, Replication, Pathogenicity and Cell Biology of Crimean Congo Hemorrhagic Fever Virus 5b. GRANT NUMBER W81XWH-04-1-0876 5c. PROGRAM ELEMENT...localization and antigenic characterization of Crimean - Congo hemorrhagic fever virus glycoproteins. J.Virol. 79: 6152-61. Ahmed, A., McFalls,

The highly virulent variola and monkeypox viruses express secreted inhibitors of type I interferon.

PubMed

Fernández de Marco, María del Mar; Alejo, Alí; Hudson, Paul; Damon, Inger K; Alcami, Antonio

2010-05-01

Variola virus (VARV) caused smallpox, one of the most devastating human diseases and the first to be eradicated, but its deliberate release represents a dangerous threat. Virulent orthopoxviruses infecting humans, such as monkeypox virus (MPXV), could fill the niche left by smallpox eradication and the cessation of vaccination. However, immunomodulatory activities and virulence determinants of VARV and MPXV remain largely unexplored. We report the molecular characterization of the VARV- and MPXV-secreted type I interferon-binding proteins, which interact with the cell surface after secretion and prevent type I interferon responses. The proteins expressed in the baculovirus system have been purified, and their interferon-binding properties characterized by surface plasmon resonance. The ability of these proteins to inhibit a broad range of interferons was investigated to identify potential adaptation to the human immune system. Furthermore, we demonstrate by Western blot and activity assays the expression of the type I interferon inhibitor during VARV and MPXV infections. These findings are relevant for the design of new vaccines and therapeutics to smallpox and emergent virulent orthopoxviruses because the type I interferon-binding protein is a major virulence factor in animal models, vaccination with this protein induces protective immunity, and its neutralization prevents disease progression.

Susceptibility of Monkeypox virus aerosol suspensions in a rotating chamber

PubMed Central

Verreault, Daniel; Killeen, Stephanie Z.; Redmann, Rachel K.; Roy, Chad. J.

2012-01-01

Summary Viral aerosols can have a major impact on public health and on the dynamics of infection. Once aerosolized, viruses are subjected to various stress factors and their integrity and potential of infectivity can be altered. Empirical characterization is needed in order to predict more accurately the fate of these bioaerosols both for short term and long term suspension in the air. Here the susceptibility to aerosolization of the monkeypox virus (MPXV), associated with emerging zoonotic diseases, was studied using a 10.7 liter rotating chamber. This chamber was built to fit inside a Class three biological safety cabinet, specifically for studying airborne biosafety level three (BSL3) microorganisms. Airborne viruses were detected by culture and quantitative polymerase chain reaction (qPCR) after up to 90 hours of aging. Viral concentrations detected dropped by two logs for culture analysis and by one log for qPCR analysis within the first 18 hours of aging; viral concentrations were stable between 18 and 90 hours, suggesting a potential for the MPXV to retain infectivity in aerosols for more than 90 hours. The rotating chamber used in this study maintained viral particles airborne successfully for prolonged periods and could be used to study the susceptibility of other BSL3 microorganisms. PMID:23142251

Evaluation of monkeypox virus infection of prairie dogs (Cynomys ludovicianus) using in vivo bioluminescent imaging

USGS Publications Warehouse

Falendysz, Elizabeth A.; Londoño-Navas, Angela M.; Meteyer, Carol U.; Pussini, Nicola; Lopera, Juan G.; Osorio, Jorge E.; Rocke, Tonie E.

2014-01-01

Monkeypox (MPX) is a re-emerging zoonotic disease that is endemic in Central and West Africa, where it can cause a smallpox-like disease in humans. Despite many epidemiologic and field investigations of MPX, no definitive reservoir species has been identified. Using recombinant viruses expressing the firefly luciferase (luc) gene, we previously demonstrated the suitability of in vivo bioluminescent imaging (BLI) to study the pathogenesis of MPX in animal models. Here, we evaluated BLI as a novel approach for tracking MPX virus infection in black-tailed prairie dogs (Cynomys ludovicianus). Prairie dogs were affected during a multistate outbreak of MPX in the US in 2003 and have since been used as an animal model of this disease. Our BLI results were compared with PCR and virus isolation from tissues collected postmortem. Virus was easily detected and quantified in skin and superficial tissues by BLI before and during clinical phases, as well as in subclinical secondary cases, but was not reliably detected in deep tissues such as the lung. Although there are limitations to viral detection in larger wild rodent species, BLI can enhance the use of prairie dogs as an animal model of MPX and can be used for the study of infection, disease progression, and transmission in potential wild rodent reservoirs.

Hydrologic controls on Congo River particulate organic carbon source and reservoir age

NASA Astrophysics Data System (ADS)

Hemingway, J. D.; Schefuß, E.; Spencer, R. G.; Dinga, B. J.; Eglinton, T. I.; McIntyre, C.; Galy, V.

2016-12-01

Tropical rivers are a major source of organic matter (OM) to the coastal ocean and play a large role in the global carbon cycle. As such, it is critical to understand the sources, sinks, and transformations of OM during fluvial transit over seasonal and inter-annual timescales. Here we present dissolved organic carbon (DOC) concentrations, particulate OM (POM) composition (δ13C, δ15N, Δ14C, N/C), and glycerol dialkyl glycerol tetraether (GDGT) biomarker distributions from a 34-month time-series near the mouth of the Congo River. An end-member mixing model based on δ13C and N/C indicates that exported POM is consistently dominated by C3 tropical rainforest soil inputs, with increasing contributions by C3 tropical plant vegetation and decreasing contributions by autochthonous phytoplankton at high discharge. Calculated Δ14C values of the C3-soil end member reveal significant and variable pre-aging prior to export, especially during the year 2011 when southern-hemisphere discharge reached record lows (mean = -176‰, standard deviation = 93‰). In contrast, Δ14C values were stable near -50‰ between January and June 2013 when southern-hemisphere discharge was highest. These results indicate that headwater POM is diluted and/or overprinted by pre-aged soils during transit through the Cuvette Congolaise swamp forest, while left-bank tributaries export significantly less pre-aged material. GDGT distributions are in agreement, as the methylation and cyclization of branched tetraethers and the GDGT-0/crenarchaeol ratio reflect a significant incorporation of compounds produced in permanently inundated Cuvette Congolaise swamp-forest soils when discharge through this region is high, especially in 2011. This study provides a mechanistic link between hydrology and carbon cycling in the world's second largest tropical river and suggests that, if recent observed decreases in springtime precipitation over the Congo basin persist, future hydrologic conditions will further bias toward export of protracted, swamp-forest-derived OM sources.

New Mycobacterium tuberculosis Complex Sublineage, Brazzaville, Congo

PubMed Central

Malm, Sven; Linguissi, Laure S. Ghoma; Tekwu, Emmanuel M.; Vouvoungui, Jeannhey C.; Kohl, Thomas A.; Beckert, Patrick; Sidibe, Anissa; Rüsch-Gerdes, Sabine; Madzou-Laboum, Igor K.; Kwedi, Sylvie; Penlap Beng, Véronique; Frank, Matthias; Ntoumi, Francine

2017-01-01

Tuberculosis is a leading cause of illness and death in Congo. No data are available about the population structure and transmission dynamics of the Mycobacterium tuberculosis complex strains prevalent in this central Africa country. On the basis of single-nucleotide polymorphisms detected by whole-genome sequencing, we phylogenetically characterized 74 MTBC isolates from Brazzaville, the capital of Congo. The diversity of the study population was high; most strains belonged to the Euro-American lineage, which split into Latin American Mediterranean, Uganda I, Uganda II, Haarlem, X type, and a new dominant sublineage named Congo type (n = 26). Thirty strains were grouped in 5 clusters (each within 12 single-nucleotide polymorphisms), from which 23 belonged to the Congo type. High cluster rates and low genomic diversity indicate recent emergence and transmission of the Congo type, a new Euro-American sublineage of MTBC. PMID:28221129

New Mycobacterium tuberculosis Complex Sublineage, Brazzaville, Congo.

PubMed

Malm, Sven; Linguissi, Laure S Ghoma; Tekwu, Emmanuel M; Vouvoungui, Jeannhey C; Kohl, Thomas A; Beckert, Patrick; Sidibe, Anissa; Rüsch-Gerdes, Sabine; Madzou-Laboum, Igor K; Kwedi, Sylvie; Penlap Beng, Véronique; Frank, Matthias; Ntoumi, Francine; Niemann, Stefan

2017-03-01

Tuberculosis is a leading cause of illness and death in Congo. No data are available about the population structure and transmission dynamics of the Mycobacterium tuberculosis complex strains prevalent in this central Africa country. On the basis of single-nucleotide polymorphisms detected by whole-genome sequencing, we phylogenetically characterized 74 MTBC isolates from Brazzaville, the capital of Congo. The diversity of the study population was high; most strains belonged to the Euro-American lineage, which split into Latin American Mediterranean, Uganda I, Uganda II, Haarlem, X type, and a new dominant sublineage named Congo type (n = 26). Thirty strains were grouped in 5 clusters (each within 12 single-nucleotide polymorphisms), from which 23 belonged to the Congo type. High cluster rates and low genomic diversity indicate recent emergence and transmission of the Congo type, a new Euro-American sublineage of MTBC.

Central African Security: Conflict in the Congo

DTIC Science & Technology

2002-01-01

strategically important Democratic Republic of the Congo (DRC). We were indeed fortunate to have as authors presenters Dr. Crawford Young, Dr. Herb...of this situation is essential for intelligence analysts and for policymakers trying to define and protect U.S. interests in the region. Importance of...DRC The Democratic Republic of the Congo, formerly known as Zaire and the Belgian Congo, has long been one of the most strategically important

How did bonobos come to range south of the congo river? Reconsideration of the divergence of Pan paniscus from other Pan populations.

PubMed

Takemoto, Hiroyuki; Kawamoto, Yoshi; Furuichi, Takeshi

2015-01-01

While investigating the genetic structure in wild bonobos,(1) we realized that the widely accepted scenario positing that the Pleistocene appearance of the Congo River separated the common ancestor of chimpanzees (Pan troglodytes) and bonobos (P. paniscus) into two species is not supported by recent geographical knowledge about the formation of the Congo River. We explored the origin of bonobos using a broader biogeographical perspective by examining local faunas in the central African region. The submarine Congo River sediments and paleotopography of central Africa show that the Congo River has functioned as a geographical barrier for the last 34 million years. This evidence allows us to hypothesize that when the river was first formed, the ancestor of bonobos did not inhabit the current range of the species on the left bank of the Congo River but that, during rare times when the Congo River discharge decreased during the Pleistocene, one or more founder populations of ancestral Pan paniscus crossed the river to its left bank. The proposed scenario for formation of the Congo River and the corridor hypothesis for an ancestral bonobo population is key to understanding the distribution of great apes and their evolution. © 2015 Wiley Periodicals, Inc.

New Species of the Spider Genus Cheiracanthium from Continental Africa
(Araneae: Eutichuridae).

PubMed

Lotz, L N

2015-06-17

Eleven new species of Cheiracanthium, C. boendense sp. nov. (Democratic Republic of Congo), C. falcis sp. nov. (Gabon), C. foordi sp. nov. (South Africa), C. ghanaense sp. nov. (Ghana), C. kabalense sp. nov. (Uganda), C. kakamega sp. nov. (Kenya), C. kakumense sp. nov. (Democratic Republic of Congo, Ivory Coast, Ghana), C. lukiense sp. nov. (Democratic Republic of Congo), C. mayombense sp. nov. (Democratic Republic of Congo), C. shilabira sp. nov. (Democratic Republic of Congo, Kenya) and C. tanzanense sp. nov. (Tanzania) are described. Males of C. punctipedellum Caporiacco, 1949, C. sansibaricum Strand, 1907 and C. schenkeli Caporiacco, 1949 are described for the first time.

Future Discounting in Congo Basin Hunter-Gatherers Declines with Socio-Economic Transitions.

PubMed

Salali, Gul Deniz; Migliano, Andrea Bamberg

2015-01-01

Humans have a tendency to discount the future; that is we value small, short-term rewards over larger, long-term rewards. The degree of future discounting, however, changes in response to socio-ecological factors. Here, we study Mbendjele BaYaka hunter-gatherers of northern Congo and their farmer neighbours to investigate adaptations in inter-temporal preferences in humans. We argue that in immediate-return systems, where food storage is absent and egalitarianism is enforced through levelling mechanisms, future discounting is an adaptive strategy to prevent wealth accumulation and the emergence of hierarchies. This ensures food sharing and allows for survival in unpredictable environments where there is risk of an energy shortfall. On the other hand, when food storage is made possible by the emergence of agriculture or as seen in some delayed-return hunter-gatherer populations, wealth accumulation, hierarchies and lower discount rates become the adaptive strategy. Therefore, individuals in immediate-return, egalitarian societies will discount the future more than those in non-egalitarian, delayed-return societies. Consistent with the predictions we found that market integration and socio-economic transitions decrease the future discounting in Mbendjele hunter-gatherers. Our measures of socio-economic differences marked this transition in hunter-gatherers living in a logging town. The degree of future-discounting was the same between more market-integrated hunter-gatherers and their farmer neighbours.

Congo (Brazzaville) Country Analysis Brief

EIA Publications

2014-01-01

Congo (Brazzaville) is among the top five oil producers in Sub-Saharan Africa. Oil production comes almost entirely from offshore oil fields. Congo exports almost all of its oil production, and the largest recipients are China and the European Union.

Quaternary ostracodes and molluscs from the Rukwa Basin (Tanzania) and their evolutionary and paleobiogeographic implications

USGS Publications Warehouse

Cohen, Andrew S.; Van Bocxlaer, Bert; Todd, Jonathan A.; McGlue, Michael; Michel, Ellinor; Nkotagu, Hudson H.; Grove, A.T.; Delvaux, Damien

2013-01-01

Much of the spectacular biodiversity of the African Great Lakes is endemic to single lake basins so that the margins of these basins or their lakes coincide with biogeographic boundaries. Longstanding debate surrounds the evolution of these endemic species, the stability of bioprovinces, and the exchange of faunas between them over geologic time as the rift developed. Because these debates are currently unsettled, we are uncertain of how much existing distribution patterns are determined by modern hydrological barriers versus reflecting past history. This study reports on late Quaternary fossils from the Rukwa Basin and integrates geological and paleoecological data to explore faunal exchange between freshwater bioprovinces, in particular with Lake Tanganyika. Lake Rukwa's water level showed large fluctuations over the last 25 ky, and for most of this period the lake contained large habitat diversity, with different species assemblages and taphonomic controls along its northern and southern shores. Comparison of fossil and modern invertebrate assemblages suggests faunal persistence through the Last Glacial Maximum, but with an extirpation event that occurred in the last 5 ky. Some of the molluscs and ostracodes studied here are closely related to taxa (or part of clades) that are currently endemic to Lake Tanganyika, but others testify to wider and perhaps older faunal exchanges between the Rukwa bioprovince and those of Lake Malawi and the Upper Congo (in particular Lake Mweru). The Rukwa Basin has a long history of rifting and lacustrine conditions and, at least temporarily, its ecosystems appear to have functioned as satellites to Lake Tanganyika in which intralacustrine speciation occurred. Paleontological studies of the Rukwa faunas are particularly relevant because of the basin's important role in the late Cenozoic biogeography of tropical Africa, and because many of the molecular traces potentially revealing this history would have been erased in the late Holocene extirpation.

Study of hydrological extremes - floods and droughts in global river basins using satellite data and model output

NASA Astrophysics Data System (ADS)

Lakshmi, V.; Fayne, J.; Bolten, J. D.

2016-12-01

We will use satellite data from TRMM (Tropical Rainfall Measurement Mission), AMSR (Advanced Microwave Scanning Radiometer), GRACE (Gravity Recovery and Climate Experiment) and MODIS (Moderate Resolution Spectroradiometer) and model output from NASA GLDAS (Global Land Data Assimilation System) to understand the linkages between hydrological variables. These hydrological variables include precipitation soil moisture vegetation index surface temperature ET and total water. We will present results for major river basins such as Amazon, Colorado, Mississippi, California, Danube, Nile, Congo, Yangtze Mekong, Murray-Darling and Ganga-Brahmaputra.The major floods and droughts in these watersheds will be mapped in time and space using the satellite data and model outputs mentioned above. We will analyze the various hydrological variables and conduct a synergistic study during times of flood and droughts. In order to compare hydrological variables between river basins with vastly different climate and land use we construct an index that is scaled by the climatology. This allows us to compare across different climate, topography, soils and land use regimes. The analysis shows that the hydrological variables derived from satellite data and NASA models clearly reflect the hydrological extremes. This is especially true when data from different sensors are analyzed together - for example rainfall data from TRMM and total water data from GRACE. Such analyses will help to construct prediction tools for water resources applications.

Is Congo red an amyloid-specific dye?

PubMed

Khurana, R; Uversky, V N; Nielsen, L; Fink, A L

2001-06-22

Congo red (CR) binding, monitored by characteristic yellow-green birefringence under crossed polarization has been used as a diagnostic test for the presence of amyloid in tissue sections for several decades. This assay is also widely used for the characterization of in vitro amyloid fibrils. In order to probe the structural specificity of Congo red binding to amyloid fibrils we have used an induced circular dichroism (CD) assay. Amyloid fibrils from insulin and the variable domain of Ig light chain demonstrate induced CD spectra upon binding to Congo red. Surprisingly, the native conformations of insulin and Ig light chain also induced Congo red circular dichroism, but with different spectral shapes than those from fibrils. In fact, a wide variety of native proteins exhibited induced CR circular dichroism indicating that CR bound to representative proteins from different classes of secondary structure such as alpha (citrate synthase), alpha + beta (lysozyme), beta (concavalin A), and parallel beta-helical proteins (pectate lyase). Partially folded intermediates of apomyoglobin induced different Congo red CD bands than the corresponding native conformation, however, no induced CD bands were observed with unfolded protein. Congo red was also found to induce oligomerization of native proteins, as demonstrated by covalent cross-linking and small angle x-ray scattering. Our data suggest that Congo red is sandwiched between two protein molecules causing protein oligomerization. The fact that Congo red binds to native, partially folded conformations and amyloid fibrils of several proteins shows that it must be used with caution as a diagnostic test for the presence of amyloid fibrils in vitro.

One step effective removal of Congo Red in chitosan nanoparticles by encapsulation

NASA Astrophysics Data System (ADS)

Alver, Erol; Bulut, Mehmet; Metin, Ayşegül Ülkü; Çiftçi, Hakan

2017-01-01

Chitosan nanoparticles (CNPs) were prepared with ionotropic gelation between chitosan and tripolyphosphate for the removal of Congo Red. The production of chitosan nanoparticles and the dye removal process was carried out in one-step. The removal efficiency of Congo Red by encapsulation within chitosan from the aqueous solution and its storage stability are examined at different pH values. The influence of some parameters such as the initial dye concentration, pH value of the dye solution, electrolyte concentration, tripolyphosphate concentration, mixing time and speed on the encapsulation is examined. Congo Red removal efficiency and encapsulation capacity of chitosan nanoparticles were determined as above 98% and 5107 mg Congo Red/g chitosan, respectively.

Proteomic Basis of the Antibody Response to Monkeypox Virus Infection Examined in Cynomolgus Macaques and a Comparison to Human Smallpox Vaccination

DTIC Science & Technology

2010-12-30

collected after challenges were gamma- irradiated (6 Mrad) to destroy any infectious virus. Previous results indicated minimal damage to serum immuno...in Sf9 insect cells using Gateway baculovirus expression (Invitrogen). All ORF clones were fully sequenced. Recombinant proteins carried GST-tags and... insect cell expression, increased the likelihood that all products were correctly folded and functional. Successfully cloned, expressed and size

Designing Medical Facilities to Care for Patients with Highly Hazardous Communicable Diseases

DTIC Science & Technology

2017-07-14

patients infected with monkeypox.20 [REF: Reynolds] The 2015 outbreak of Middle Eastern Respiratory Syndrome Corona virus (MERS- CoV), in Korea led to...adjunct. Units have used sealed floors and walls for ease of decontamination after patient discharge , although the housekeeping is frequently done...handling. When a patient is discharged or succumbs to the disease, it is useful to have procedures in place for decontaminating the facility and for

Influence of the amyloid dye Congo red on curli, cellulose, and the extracellular matrix in E. coli during growth and matrix purification.

PubMed

Reichhardt, Courtney; McCrate, Oscar A; Zhou, Xiaoxue; Lee, Jessica; Thongsomboon, Wiriya; Cegelski, Lynette

2016-11-01

Microbial biofilms are communities of cells characterized by a hallmark extracellular matrix (ECM) that confers functional attributes to the community, including enhanced cohesion, adherence to surfaces, and resistance to external stresses. Understanding the composition and properties of the biofilm ECM is crucial to understanding how it functions and protects cells. New methods to isolate and characterize ECM are emerging for different biofilm systems. Solid-state nuclear magnetic resonance was used to quantitatively track the isolation of the insoluble ECM from the uropathogenic Escherichia coli strain UTI89 and understand the role of Congo red in purification protocols. UTI89 assembles amyloid-integrated biofilms when grown on YESCA nutrient agar. The ECM contains curli amyloid fibers and a modified form of cellulose. Biofilms formed by UTI89 and other E. coli and Salmonella strains are often grown in the presence of Congo red to visually emphasize wrinkled agar morphologies and to score the production of ECM. Congo red is a hallmark amyloid-binding dye and binds to curli, yet also binds to cellulose. We found that growth in Congo red enabled more facile extraction of the ECM from UTI89 biofilms and facilitates isolation of cellulose from the curli mutant, UTI89ΔcsgA. Yet, Congo red has no influence on the isolation of curli from curli-producing cells that do not produce cellulose. Sodium dodecyl sulfate can remove Congo red from curli, but not from cellulose. Thus, Congo red binds strongly to cellulose and possibly weakens cellulose interactions with the cell surface, enabling more complete removal of the ECM. The use of Congo red as an extracellular matrix purification aid may be applied broadly to other organisms that assemble extracellular amyloid or cellulosic materials. Graphical abstract Solid-state NMR was used to quantitatively track the isolation of the insoluble amyloid-associated ECM from uropathogenic E. coli and understand the role of Congo red in purification protocols.

Congo red agar, a differential medium for Aeromonas salmonicida, detects the presence of the cell surface protein array involved in virulence.

PubMed Central

Ishiguro, E E; Ainsworth, T; Trust, T J; Kay, W W

1985-01-01

Strains of the fish pathogen Aeromonas salmonicida which possess the cell surface protein array known as the A-layer (A+) involved in virulence formed deep red colonies on tryptic soy agar containing 30 micrograms of Congo red per ml. These were readily distinguished from colorless or light orange colonies of avirulent mutants lacking A-layer (A-). The utility of Congo red agar for quantifying A+ and A- cells in the routine assessment of culture virulence was demonstrated. Intact A+ cells adsorbed Congo red, whereas A- mutants did not bind Congo red unless first permeabilized with EDTA. The dye-binding component of A+ cells was shown to be the 50,000-Mr A-protein component of the surface array. Purified A-protein avidly bound Congo red at a dye-to-protein molar ratio of about 30 by a nonspecific hydrophobic mechanism enhanced by high salt concentrations. Neither A+ nor A- cells adsorbed to Congo red-Sepharose columns at low salt concentrations. On the other hand, A+ (but not A-) cells were avidly bound at high salt concentrations. Images PMID:3934141

Biosorption of Congo Red from aqueous solution by Bacillus weihenstephanensis RI12; effect of SPB1 biosurfactant addition on biodecolorization potency.

PubMed

Mnif, Inès; Fendri, Raouia; Ghribi, Dhouha

2015-01-01

Bacillus weihenstephanensis RI12, isolated from hydrocarbon contaminated soil, was assessed for Congo Red bio-treatment potency. Results suggested the potential of this bacterium for use in effective treatment of Congo Red contaminated wastewaters under shaking conditions at acidic and neutral pH value. The strain could tolerate higher doses of dyes as it could decolorize up to 1,000 mg/l of Congo Red. When used as microbial surfactant to enhance Congo Red biodecolorization, Bacillus subtilis SPB1-derived lipopeptide accelerated the decolorization rate and maximized the decolorization efficiency at an optimal concentration of biosurfactant of about 0.075%. Studies ensured that Congo Red removal by this strain could be due to an adsorption phenomena. Germination potencies of tomato seeds using the treated dyes under different conditions showed the efficient biotreatment of the azo dye Congo Red especially with the addition of SPB1 biosurfactant. To conclude, the addition of SPB1 bioemulsifier reduced energy costs by reducing the effective decolorization period; the biosurfactant stimulated bacterial decolorization method may provide a highly efficient, inexpensive and time-saving procedure in the treatment of textile effluents.

31 CFR 547.201 - Prohibited transactions involving blocked property.

Code of Federal Regulations, 2013 CFR

2013-07-01

... THE CONGO SANCTIONS REGULATIONS Prohibitions § 547.201 Prohibited transactions involving blocked... the Democratic Republic of the Congo that impedes the disarmament, repatriation, or resettlement of... children in armed conflict in the Democratic Republic of the Congo in violation of applicable international...

31 CFR 547.201 - Prohibited transactions involving blocked property.

Code of Federal Regulations, 2014 CFR

2014-07-01

... THE CONGO SANCTIONS REGULATIONS Prohibitions § 547.201 Prohibited transactions involving blocked... the Democratic Republic of the Congo that impedes the disarmament, repatriation, or resettlement of... children in armed conflict in the Democratic Republic of the Congo in violation of applicable international...

31 CFR 547.201 - Prohibited transactions involving blocked property.

Code of Federal Regulations, 2012 CFR

2012-07-01

... THE CONGO SANCTIONS REGULATIONS Prohibitions § 547.201 Prohibited transactions involving blocked... the Democratic Republic of the Congo that impedes the disarmament, repatriation, or resettlement of... children in armed conflict in the Democratic Republic of the Congo in violation of applicable international...

31 CFR 547.201 - Prohibited transactions involving blocked property.

Code of Federal Regulations, 2011 CFR

2011-07-01

... THE CONGO SANCTIONS REGULATIONS Prohibitions § 547.201 Prohibited transactions involving blocked... the Democratic Republic of the Congo that impedes the disarmament, repatriation, or resettlement of... children in armed conflict in the Democratic Republic of the Congo in violation of applicable international...

31 CFR 547.201 - Prohibited transactions involving blocked property.

Code of Federal Regulations, 2010 CFR

2010-07-01

... THE CONGO SANCTIONS REGULATIONS Prohibitions § 547.201 Prohibited transactions involving blocked... the Democratic Republic of the Congo that impedes the disarmament, repatriation, or resettlement of... children in armed conflict in the Democratic Republic of the Congo in violation of applicable international...

Evaluation of the chitin-binding dye Congo red as a selection agent for the isolation, classification, and enumeration of ascomycete yeasts.

PubMed

Linder, Tomas

2018-05-01

Thirty-nine strains of ascomycete yeasts representing 35 species and 33 genera were tested for their ability to grow on solid agar medium containing increasing concentrations of the chitin-binding dye Congo red. Six strains were classified as hypersensitive (weak or no growth at 10 mg/l Congo red), five were moderately sensitive (weak or no growth at 50 mg/l), three were moderately tolerant (weak or no growth at 100 mg/l), while the remaining 25 strains were classified as resistant (robust growth at ≥ 100 mg/l) with 20 of these strains classified as hyper-resistant (robust growth at 200 mg/l). Congo red growth phenotypes were consistent within some families but not others. The frequency of Congo red resistance among ascomycete yeasts was deemed too high for the practical use of Congo red as a selection agent for targeted isolation, but can be useful for identification and enumeration of yeasts.

Countermeasures to the bioterrorist threat of smallpox.

PubMed

Jahrling, Peter B; Fritz, Elizabeth A; Hensley, Lisa E

2005-12-01

Variola, the agent of smallpox, is a bioterrorist threat, as is monkeypox virus, which also occurs naturally in Africa. Development of countermeasures, in the form of improved vaccines, antiviral drugs, and other therapeutic strategies are a high priority. Recent advances in molecular biology and in animal model development have provided fresh insight into the virulence determinants for smallpox and the pathophysiology of disease. The complex replication cycle for orthopoxviruses, and the pivotal role for viral-specific immunomodulatory proteins which contribute to escape from immunologic surveillance, provide many unique targets for therapeutic intervention. The "toxemia" of smallpox has been elucidated in part by variola-infected primate studies which revealed the central role of apoptosis and the evolution of a cytokine storm leading to hemorrhagic diathesis, resembling fulminent "black" smallpox. This suggests a potential role for therapeutic strategies developed for septic shock, in treatment of smallpox. Drugs licensed for other viruses which share molecular targets with orthopoxviruses (e.g. Cidofovir) or cancer drugs (e.g. Gleevec and other tyrosine kinase inhibitors) have immediate application for treatment of smallpox and monkeypox and provide leads for second generation drugs with higher therapeutic indices. Recent advances in identification of virulence determinants and immune evasion genes facilitate the design of alternative vaccines to replace live vaccinia strains that are unsuitable for a large proportion of individuals in a mass immunization campaign.

A Preliminary Assessment of Silver Nanoparticle Inhibition of Monkeypox Virus Plaque Formation

NASA Astrophysics Data System (ADS)

Rogers, James V.; Parkinson, Christopher V.; Choi, Young W.; Speshock, Janice L.; Hussain, Saber M.

2008-04-01

The use of nanotechnology and nanomaterials in medical research is growing. Silver-containing nanoparticles have previously demonstrated antimicrobial efficacy against bacteria and viral particles. This preliminary study utilized an in vitro approach to evaluate the ability of silver-based nanoparticles to inhibit infectivity of the biological select agent, monkeypox virus (MPV). Nanoparticles (10 80 nm, with or without polysaccharide coating), or silver nitrate (AgNO3) at concentrations of 100, 50, 25, and 12.5 μg/mL were evaluated for efficacy using a plaque reduction assay. Both Ag-PS-25 (polysaccharide-coated, 25 nm) and Ag-NP-55 (non-coated, 55 nm) exhibited a significant ( P ≤ 0.05) dose-dependent effect of test compound concentration on the mean number of plaque-forming units (PFU). All concentrations of silver nitrate (except 100 μg/mL) and Ag-PS-10 promoted significant ( P ≤ 0.05) decreases in the number of observed PFU compared to untreated controls. Some nanoparticle treatments led to increased MPV PFU ranging from 1.04- to 1.8-fold above controls. No cytotoxicity (Vero cell monolayer sloughing) was caused by any test compound, except 100 μg/mL AgNO3. These results demonstrate that silver-based nanoparticles of approximately 10 nm inhibit MPV infection in vitro, supporting their potential use as an anti-viral therapeutic.

Tracing late Quaternary tropical wetland dynamics in the Congo catchment using microbial biomarker records from deep sea fan sediments

NASA Astrophysics Data System (ADS)

Spencer-Jones, Charlotte Louise; Schefuβ, Enno; Wagner, Thomas; Handley, Luke; Talbot, Helen Marie

2014-05-01

Methane is a climatically active gas with a global warming potential 72 time that of CO2 over 20 years. Release of methane into the atmosphere has been suggested as a potential source of warming in palaeoclimate studies. This has implications for future climate as increased global temperatures could destabilise sources of sedimentary methane releasing it to the atmosphere. It is therefore important to establish the possible sinks of methane that could attenuate methane emissions. We present a high resolution record from the Congo deep sea fan (ODP 1075) of amino-bacteriohopanepolyols (amino-BHPs). The methanotrophic source of aminopentol, a biomarker for aerobic methane oxidation (AMO), in ODP 1075 is supported by compound specific δ13C isotope values of -41‰ for aminopentol precursors. High resolution intervals of isotope stages 10 to 13 (~500 to ~400 kyrs BP) confirm aminopentol to vary on glacial-interglacial timescales. High concentrations of amino-BHPs are recorded during warm, interglacial stages 11 and 13 with low concentrations of amino-BHPs during cold, glacial stages 10 and 12. This increase in AMO intensity (as suggested by aminopentol concentrations) during stages 11 and 13 is likely an imported signature from the Congo hinterland. Sediments analysed for amino-BHPs from floodplain wetlands show similar biomarker signatures as the marine sediments, suggesting a common source. Wetlands are important and widespread sub-environments in all large tropical river catchments. Their extent responds to fluctuations in humidity, which changes at glacial-interglacial and shorter time scales in response to the level of humidity. Humidity in the interior of tropical Africa has been shown to be driven by fluctuations in the difference in sea surface temperature (SST) between the subtropical and tropical South Atlantic (Schefuss et al., 2004). D-SST profiles based on UK 37 from the Angola (ODP 1082) and Congo basins (ODP 1077, Geob 1082) show an inverse relationship with amino-BHP profiles with minimum Delta-SST values coinciding high amino-BHP concentrations. Therefore periods of high humidity (as inferred from minimum Delta-SST) coincide with periods of intense AMO biomarker production and export, supporting the novel approach to use amino BHPs in marine sediments to reconstruct tropical wetland dynamics in the past. Schefuss, E., Damste, J.S.S., Jansen, J.H.F., 2004. Forcing of tropical Atlantic sea surface temperatures during the mid-Pleistocene transition. Paleoceanography 19.

78 FR 6180 - Designation of Individuals Pursuant to Executive Order 13413

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-29

... Republic of Congo.'' DATES: The designation by the Director of OFAC of the two individuals identified in... relation to the Democratic Republic of the Congo constitutes an unusual and extraordinary threat to the...-Marie Lugerero); DOB September 17, 1966; POB Democratic Republic of the Congo (individual) [DRCONGO] 2...

78 FR 2722 - Designation of Individuals Pursuant to Executive Order 13413

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-14

... Republic of Congo.'' DATES: The designation by the Director of OFAC of the two individuals identified in... relation to the Democratic Republic of the Congo constitutes an unusual and extraordinary threat to the... 1978; POB Bunagana, Rutshuru territory, Democratic Republic of the Congo; Colonel (individual) [DRCONGO...

Secretion of Ipa proteins by Shigella flexneri: inducer molecules and kinetics of activation.

PubMed Central

Bahrani, F K; Sansonetti, P J; Parsot, C

1997-01-01

The type III Mxi-Spa secretion machinery of Shigella flexneri is responsible for secretion of Ipa proteins, which are involved in the entry of bacteria into epithelial cells. Ipa proteins accumulate within bacteria growing in laboratory media, and their secretion is activated upon contact of bacteria with eukaryotic cells. In this study, we have identified a group of chemical compounds, including Congo red, Evans blue, and direct orange, which are able to induce secretion of Ipa proteins by bacteria suspended in phosphate-buffered saline. Parameters of kinetics of activation of Ipa secretion by Congo red were determined by measuring by enzyme-linked immunosorbent assay the amount of IpaC secreted and by investigating the increase in susceptibility of Ipa proteins to proteinase K degradation. Ipa secretion occurred at 37 degrees C, was obtained with 5 to 10 microM Congo red, and was complete within 30 min. In addition, activation of Ipa secretion by Congo red was observed with bacteria harvested throughout the exponential phase of growth but not with bacteria in the stationary phase. The interactions of Congo red and Congo red-related compounds with the Mxi-Spa secretion apparatus might be specific hydrophobic interactions similar to those involved in binding of Congo red to amyloid proteins. PMID:9316999

Molecular surveillance of Plasmodium falciparum resistance to artemisinin-based combination therapies in the Democratic Republic of Congo.

PubMed

Mvumbi, Dieudonné Makaba; Bobanga, Thierry Lengu; Kayembe, Jean-Marie Ntumba; Mvumbi, Georges Lelo; Situakibanza, Hippolyte Nani-Tuma; Benoit-Vical, Françoise; Melin, Pierrette; De Mol, Patrick; Hayette, Marie-Pierre

2017-01-01

Malaria is a major public health problem in the Democratic Republic of Congo. Despite progress achieved over the past decade in the fight against malaria, further efforts have to be done such as in the surveillance and the containment of Plasmodium falciparum resistant strains. We investigated resistance to artemisinin-based combination therapies currently in use in Democratic Republic of Congo by surveying molecular polymorphisms in three genes: pfcrt, pfmdr1 and pfk13 to explore possible emergence of amodiaquine, lumefantrine or artemisinin resistance in Democratic Republic of Congo. This study essentially revealed that resistance to chloroquine is still decreasing while polymorphism related to amodiaquine resistance seems to be not present in Democratic Republic of Congo, that three samples, located in the east of the country, harbor Pfmdr1 amplification and that none of the mutations found in South-East Asia correlated with artemisinine resistance have been found in Democratic Republic of Congo. But new mutations have been identified, especially the M476K, occurred in the same position that the M476I previously identified in the F32-ART strain, strongly resistant to artemisinine. Antimalarial first-line treatments currently in use in Democratic Republic of Congo are not associated with emergence of molecular markers of resistance.

Secular spring rainfall variability at local scale over Ethiopia: trend and associated dynamics

NASA Astrophysics Data System (ADS)

Tsidu, Gizaw Mengistu

2017-10-01

Spring rainfall secular variability is studied using observations, reanalysis, and model simulations. The joint coherent spatio-temporal secular variability of gridded monthly gauge rainfall over Ethiopia, ERA-Interim atmospheric variables and sea surface temperature (SST) from Hadley Centre Sea Ice and SST (HadISST) data set is extracted using multi-taper method singular value decomposition (MTM-SVD). The contemporaneous associations are further examined using partial Granger causality to determine presence of causal linkage between any of the climate variables. This analysis reveals that only the northwestern Indian Ocean secular SST anomaly has direct causal links with spring rainfall over Ethiopia and mean sea level pressure (MSLP) over Africa inspite of the strong secular covariance of spring rainfall, SST in parts of subtropical Pacific, Atlantic, Indian Ocean and MSLP. High secular rainfall variance and statistically significant linear trend show consistently that there is a massive decline in spring rain over southern Ethiopia. This happened concurrently with significant buildup of MSLP over East Africa, northeastern Africa including parts of the Arabian Peninsula, some parts of central Africa and SST warming over all ocean basins with the exception of the ENSO regions. The east-west pressure gradient in response to the Indian Ocean warming led to secular southeasterly winds over the Arabian Sea, easterly over central Africa and equatorial Atlantic. These flows weakened climatological northeasterly flow over the Arabian Sea and southwesterly flow over equatorial Atlantic and Congo basins which supply moisture into the eastern Africa regions in spring. The secular divergent flow at low level is concurrent with upper level convergence due to the easterly secular anomalous flow. The mechanisms through which the northwestern Indian Ocean secular SST anomaly modulates rainfall are further explored in the context of East Africa using a simplified atmospheric general circulation model (AGCM) coupled to mixed-layer oceanic model. The rainfall anomaly (with respect to control simulation), forced by the northwestern Indian Ocean secular SST anomaly and averaged over the 30-year period, exhibits prevalence of dry conditions over East and equatorial Africa in agreement with observation. The atmospheric response to secular SST warming anomaly led to divergent flow at low levels and subsidence at the upper troposphere over regions north of 5° S on the continent and vice versa over the Indian Ocean. This surface difluence over East Africa, in addition to its role in suppressing convective activity, deprives the region of moisture supply from the Indian Ocean as well as the Atlantic and Congo basins.

Implications of global warming for the climate of African rainforests

PubMed Central

James, Rachel; Washington, Richard; Rowell, David P.

2013-01-01

African rainforests are likely to be vulnerable to changes in temperature and precipitation, yet there has been relatively little research to suggest how the regional climate might respond to global warming. This study presents projections of temperature and precipitation indices of relevance to African rainforests, using global climate model experiments to identify local change as a function of global temperature increase. A multi-model ensemble and two perturbed physics ensembles are used, one with over 100 members. In the east of the Congo Basin, most models (92%) show a wet signal, whereas in west equatorial Africa, the majority (73%) project an increase in dry season water deficits. This drying is amplified as global temperature increases, and in over half of coupled models by greater than 3% per °C of global warming. Analysis of atmospheric dynamics in a subset of models suggests that this could be partly because of a rearrangement of zonal circulation, with enhanced convection in the Indian Ocean and anomalous subsidence over west equatorial Africa, the Atlantic Ocean and, in some seasons, the Amazon Basin. Further research to assess the plausibility of this and other mechanisms is important, given the potential implications of drying in these rainforest regions. PMID:23878329

Implications of global warming for the climate of African rainforests.

PubMed

James, Rachel; Washington, Richard; Rowell, David P

2013-01-01

African rainforests are likely to be vulnerable to changes in temperature and precipitation, yet there has been relatively little research to suggest how the regional climate might respond to global warming. This study presents projections of temperature and precipitation indices of relevance to African rainforests, using global climate model experiments to identify local change as a function of global temperature increase. A multi-model ensemble and two perturbed physics ensembles are used, one with over 100 members. In the east of the Congo Basin, most models (92%) show a wet signal, whereas in west equatorial Africa, the majority (73%) project an increase in dry season water deficits. This drying is amplified as global temperature increases, and in over half of coupled models by greater than 3% per °C of global warming. Analysis of atmospheric dynamics in a subset of models suggests that this could be partly because of a rearrangement of zonal circulation, with enhanced convection in the Indian Ocean and anomalous subsidence over west equatorial Africa, the Atlantic Ocean and, in some seasons, the Amazon Basin. Further research to assess the plausibility of this and other mechanisms is important, given the potential implications of drying in these rainforest regions.

A linear geospatial streamflow modeling system for data sparse environments

USGS Publications Warehouse

Asante, Kwabena O.; Arlan, Guleid A.; Pervez, Md Shahriar; Rowland, James

2008-01-01

In many river basins around the world, inaccessibility of flow data is a major obstacle to water resource studies and operational monitoring. This paper describes a geospatial streamflow modeling system which is parameterized with global terrain, soils and land cover data and run operationally with satellite‐derived precipitation and evapotranspiration datasets. Simple linear methods transfer water through the subsurface, overland and river flow phases, and the resulting flows are expressed in terms of standard deviations from mean annual flow. In sample applications, the modeling system was used to simulate flow variations in the Congo, Niger, Nile, Zambezi, Orange and Lake Chad basins between 1998 and 2005, and the resulting flows were compared with mean monthly values from the open‐access Global River Discharge Database. While the uncalibrated model cannot predict the absolute magnitude of flow, it can quantify flow anomalies in terms of relative departures from mean flow. Most of the severe flood events identified in the flow anomalies were independently verified by the Dartmouth Flood Observatory (DFO) and the Emergency Disaster Database (EM‐DAT). Despite its limitations, the modeling system is valuable for rapid characterization of the relative magnitude of flood hazards and seasonal flow changes in data sparse settings.

Sea Surface Salinity Variability in Response to the Congo River Discharge

NASA Astrophysics Data System (ADS)

Moller, D.; Chao, Y.; Farrara, J. D.; Schumann, G.; Andreadis, K.

2014-12-01

Sea surface salinity (SSS) variability associated with the Congo River discharge is examined using Aquarius satellite-retrieved SSS data and vertical profiles of salinity measured by the Argo floats. The Congo River plume can be clearly identified in the Aquarius SSS data with a westward extension of 500 to 1000 km off the coast of the Democratic Republic of Congo (DRC). The peak amplitude of the SSS variability associated with the Congo River discharge exceeds 2.0 psu. Using the first two years of Aquarius data, a well-defined seasonal cycle is described: maximum fresh-water anomalies are found in the boreal winter and spring seasons. The fresh-water anomalies during the 2012-2013 winter and spring seasons are significantly fresher than the 2011-2012 winter and spring seasons. Vertical profiles of salinity derived from the Argo floats reveal that these fresh-water anomalies can be traced to 40 meters below the sea surface. Combining the Aquarius SSS data with the Argo vertical profiles of salinity, the 3D volume of these fresh-water anomalies can be inferred and used to estimate the Congo River discharge. Reasonably good agreement is found between the Congo River discharge as observed by a stream gauge at Kinshasa and that estimated from the combined Aquarius and Argo data, indicating that Aquarius data can be used to close the fresh-water budget between the coastal ocean and the Congo River. The precipitation minus evaporation portion of the freshwater flux is found to play a secondary role in this region.

31 CFR 547.301 - Arms or any related materiel.

Code of Federal Regulations, 2011 CFR

2011-07-01

... OF FOREIGN ASSETS CONTROL, DEPARTMENT OF THE TREASURY DEMOCRATIC REPUBLIC OF THE CONGO SANCTIONS... use by units of the army and police of the Democratic Republic of the Congo, provided that said units... the Congo; (3) Are in the process of their integration in the territory of the Democratic Republic of...

77 FR 69547 - Designation of an Individual Pursuant to Executive Order 13413

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-19

... Republic of Congo.'' DATES: The designation by the Director of OFAC of the one individual identified in... relation to the Democratic Republic of the Congo constitutes an unusual and extraordinary threat to the... Sultani); DOB 25 Dec 1973; POB Rutshuru, Democratic Republic of the Congo; Colonel (individual) [DRCONGO...

31 CFR 547.301 - Arms or any related materiel.

Code of Federal Regulations, 2013 CFR

2013-07-01

... OF FOREIGN ASSETS CONTROL, DEPARTMENT OF THE TREASURY DEMOCRATIC REPUBLIC OF THE CONGO SANCTIONS... use by units of the army and police of the Democratic Republic of the Congo, provided that said units... the Congo; (3) Are in the process of their integration in the territory of the Democratic Republic of...

78 FR 2721 - Designation of Entities Pursuant to Executive Order 13413

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-14

... Democratic Republic of Congo.'' DATES: The designation by the Director of OFAC of the two entities identified... relation to the Democratic Republic of the Congo constitutes an unusual and extraordinary threat to the.... FDLR; a.k.a. ``FOCA''; a.k.a. FORCE COMBATTANTE ABACUNGUZI), North and South Kivu, Congo, Democratic...

75 FR 37880 - Blocking of Specially Designated National Pursuant to Executive Order 13413

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-30

... the Democratic Republic of Congo''. DATES: The designation by the Director of OFAC of the one... President found that the situation in the Democratic Republic of the Congo constitutes and unusual and... BAKONDE, Jerome; a.k.a. GAKWAVU BOKANDE, Jerome; a.k.a. GAGAKWAVU, Jerome); Kinshasa, Aru, Congo...

75 FR 23847 - Blocking of Specially Designated National Pursuant to Executive Order 13413

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-04

... Conflict in the Democratic Republic of Congo''. DATES: The designation by the Director of OFAC of the five... President found that the situation in the Democratic Republic of the Congo constitutes an unusual and... Kivu, Congo, Democratic Republic of the; DOB 1973; POB Nord-Kivu, DRC; alt. POB Rwanda; nationality...

31 CFR 547.301 - Arms or any related materiel.

Code of Federal Regulations, 2014 CFR

2014-07-01

... OF FOREIGN ASSETS CONTROL, DEPARTMENT OF THE TREASURY DEMOCRATIC REPUBLIC OF THE CONGO SANCTIONS... use by units of the army and police of the Democratic Republic of the Congo, provided that said units... the Congo; (3) Are in the process of their integration in the territory of the Democratic Republic of...

31 CFR 547.301 - Arms or any related materiel.

Code of Federal Regulations, 2012 CFR

2012-07-01

... OF FOREIGN ASSETS CONTROL, DEPARTMENT OF THE TREASURY DEMOCRATIC REPUBLIC OF THE CONGO SANCTIONS... use by units of the army and police of the Democratic Republic of the Congo, provided that said units... the Congo; (3) Are in the process of their integration in the territory of the Democratic Republic of...

Effect of pH, Dosage and Concentration on the Adsorption of Congo Red onto Untreated and Treated Aluminium Dross

NASA Astrophysics Data System (ADS)

Zakaria Mohamad Zulfika, Hazielim B.; Baini, Rubiyah; Zauzi, Nur Syuhada Ahmad

2017-06-01

The adsorption of congo red onto aluminium dross was studied in batch process. The objective of this study is to adsorption capacity between untreated and treated aluminium dross in the removal of congo red. Aluminium dross was leached with 250 ml of 1% of NaOH and and precipitated with 30% H2O2. The treated aluminium dross being calcined at 600°C for 3 hours. The surface area for untreated and treated aluminium dross was 10.06 m2/g and 79.80 m2/g respectively. Then the adsorption process was carried out on an orbital shaker at 200 rpm for 4 hours. In the effect of pH, it was found that untreated removes more congo red compared to the treated while in the effect of concentration solution and dosage of adsorbent, treated aluminium dross removes more congo red. In conclusion, this adsorbent was found to be effective and economically viable in the removal of congo red in waste water treatment.

Generating daily weather data for ecosystem modelling in the Congo River Basin

NASA Astrophysics Data System (ADS)

Petritsch, Richard; Pietsch, Stephan A.

2010-05-01

Daily weather data are an important constraint for diverse applications in ecosystem research. In particular, temperature and precipitation are the main drivers for forest ecosystem productivity. Mechanistic modelling theory heavily relies on daily values for minimum and maximum temperatures, precipitation, incident solar radiation and vapour pressure deficit. Although the number of climate measurement stations increased during the last centuries, there are still regions with limited climate data. For example, in the WMO database there are only 16 stations located in Gabon with daily weather measurements. Additionally, the available time series are heavily affected by measurement errors or missing values. In the WMO record for Gabon, on average every second day is missing. Monthly means are more robust and may be estimated over larger areas. Therefore, a good alternative is to interpolate monthly mean values using a sparse network of measurement stations, and based on these monthly data generate daily weather data with defined characteristics. The weather generator MarkSim was developed to produce climatological time series for crop modelling in the tropics. It provides daily values for maximum and minimum temperature, precipitation and solar radiation. The monthly means can either be derived from the internal climate surfaces or prescribed as additional inputs. We compared the generated outputs observations from three climate stations in Gabon (Lastourville, Moanda and Mouilla) and found that maximum temperature and solar radiation were heavily overestimated during the long dry season. This is due to the internal dependency of the solar radiation estimates to precipitation. With no precipitation a cloudless sky is assumed and thus high incident solar radiation and a large diurnal temperature range. However, in reality it is cloudy in the Congo River Basin during the long dry season. Therefore, we applied a correction factor to solar radiation and temperature range based on the ratio of values on rainy days and days without rain, respectively. For assessing the impact of our correction, we simulated the ecosystem behaviour using the climate data from Lastourville, Moanda and Mouilla with the mechanistic ecosystem model Biome-BGC. Differences in terms of the carbon, nitrogen and water cycle were subsequently analysed and discussed.

Hydroclimatic Change in the Congo River Basin: Past, Present and Future169

NASA Astrophysics Data System (ADS)

Aloysius, N. R.

2016-12-01

Tropical regions provide habitat for the world's most diverse fauna and flora, sequester more atmospheric carbon and provide livelihood for millions of people. The hydrological cycle provides vital linkages for maintaining these ecosystem functions, yet, the understanding of its spatiotemporal variability is limited. Research on the hydrological cycle of the Congo River Basin (CRB), which encompasses the second largest rainforests, has been largely ignored. Global Climate Models (GCM) show limited skills in simulating CRB's climate and their future projections vary widely. Yet, GCMs provide the most plausible scenarios of future climate, based upon which changes in hydrologic fluxes can be predicted with the aid hydrological models. In order to address the gaps in knowledge and to highlight the research needs, we i) developed a spatially explicit hydrological model suitable for describing key hydrological processes, ii) evaluated the performance of GCMs in simulating precipitation and temperature in the region, iii) developed a set of climate change scenarios for the CRB and iv) developed a simplified modeling framework to quantify water management options for rain-fed agriculture with the objective of achieving the triple goals of sustainable development: food security, poverty alleviation and ecosystem conservation. The hydrology model, which was validated with observed stream flows at 50 locations, satisfactorily characterizes spatiotemporal variability of key fluxes. Our evaluation of 25 GCM outputs reveal that many GCMs poorly simulate regional precipitation. We implemented a statistical bias-correction method to develop precipitation and temperature projections for two future greenhouse gas emission scenarios. These climate forcings were, then, used to drive the hydrology model. Our results show that the near-term projections are not affected by emission scenarios. However, towards the mid-21st century, projections are emission scenario dependent. Available freshwater resources are projected to increase in the CRB, except in the semiarid southeast. Our findings have wider implications for climate change assessment and water resource management, because the region, with high population growth and limited capacity to adapt, are primary targets of land and water grabs. 155

Comparison of lightning activity in the two most active areas of the Congo Basin

NASA Astrophysics Data System (ADS)

Kigotsi, Jean K.; Soula, Serge; Georgis, Jean-François

2018-02-01

A comparison of the lightning activity in the two most active areas (Area_max for the main maximum and Area_sec for the secondary maximum) of the Congo Basin is made with data obtained by the World Wide Lightning Location Network (WWLLN) during 2012 and 2013. Both areas of same size (5° × 5°) exhibit flash counts in a ratio of about 1.32 for both years and very different distributions of the flash rate density (FRD) with maximums in a ratio of 1.94 and 2.59 for 2012 and 2013, respectively. The FRD is much more widely distributed in Area_sec, which means the whole area contributes more or less equal to the lightning activity. The diurnal cycle is much more pronounced in Area_max than in Area_sec with a ratio between the maximum and the minimum of 15.4 and 4.7, respectively. However, the minimum and maximum of the hourly flash rates are observed roughly at the same time in both areas, between 07:00 and 09:00 UTC and between 16:00 and 17:00 UTC, respectively. In Area_sec the proportion of days with low lightning rate (0-1000 flashes per day) is much larger (˜ 45 % in 2013) compared to Area_max (˜ 23 % in 2013). In Area_max the proportion of days with moderate lightning rate (1001-6000 flashes per day) is larger (˜ 68.5 % in 2013) compared to Area_sec (˜ 46 % in 2013). The very intense convective events are slightly more numerous in Area_sec. In summary, the thunderstorm activity in Area_sec is more variable at different scales of time (annually and daily), in intensity and in location. Area_max combines two favourable effects for thunderstorm development, the convergence associated with the African easterly jet of the Southern Hemisphere (AEJ-S) and a geographic effect due to the orography and the presence of a lake. The location of the strong convection in Area_sec is modulated by the distance of westward propagation/regeneration of mesoscale convective systems (MCSs) in relation to the phase of Kelvin waves.

Learning from Experience: The Public Health Response to West Nile Virus, SARS, Monkeypox, and Hepatitis A Outbreaks in the United States

DTIC Science & Technology

2005-01-01

chickens in May and June 2002, with the first human cases showing up in hospital emergency departments in July. The Louisiana outbreak lasted until... Mexico , that had been used in a salsa served in a Beaver County restaurant. A total of 660 cases were reported, resulting in 3 deaths. Table...imported from Africa Contaminated green onions imported from Mexico Transmission Vector-borne (mosquitoes) Respiratory droplets (person-to

Experimental Infection of Cynomolgus Macaques (Macaca fascicularis) with Aerosolized Monkeypox Virus

DTIC Science & Technology

2010-09-01

and interstitial fibrosis, and fibrous pleural adhesions. Other lesions included lymphoid hyperplasia and plasmacytosis, and chronic periadnexal and...inflammation in the lung and mediastinal lymph nodes, chronic inflammation centered on bronchi and vessels, type II pneumocyte hyperplasia , pleural...2/6 33 1/6 16 1/3 33 spleen splenitis, necrotizing 1/3 33 2/6 33 4/6 66 2/3 66 lymphoid depletion 2/3 66 3/6 50 3/6 50 2/3 66 lymphoid hyperplasia * 0

Hydroclimate variability in the Nile River Basin during the past 28,000 years

NASA Astrophysics Data System (ADS)

Castañeda, Isla S.; Schouten, Stefan; Pätzold, Jürgen; Lucassen, Friedrich; Kasemann, Simone; Kuhlmann, Holger; Schefuß, Enno

2016-03-01

It has long been known that extreme changes in North African hydroclimate occurred during the late Pleistocene yet many discrepancies exist between sites regarding the timing, duration and abruptness of events such as Heinrich Stadial (HS) 1 and the African Humid Period (AHP). The hydroclimate history of the Nile River is of particular interest due to its lengthy human occupation history yet there are presently few continuous archives from the Nile River corridor, and pre-Holocene studies are rare. Here we present new organic and inorganic geochemical records of Nile Basin hydroclimate from an eastern Mediterranean (EM) Sea sediment core spanning the past 28 ka BP. Our multi-proxy records reflect the fluctuating inputs of Blue Nile versus White Nile material to the EM Sea in response to gradual changes in local insolation and also capture abrupt hydroclimate events driven by remote climate forcings, such as HS1. We find strong evidence for extreme aridity within the Nile Basin evolving in two distinct phases during HS1, from 17.5 to 16 ka BP and from 16 to 14.5 ka BP, whereas peak wet conditions during the AHP are observed from 9 to 7 ka BP. We find that zonal movements of the Congo Air Boundary (CAB), and associated shifts in the dominant moisture source (Atlantic versus Indian Ocean moisture) to the Nile Basin, likely contributed to abrupt hydroclimate variability in northern East Africa during HS1 and the AHP as well as to non-linear behavior of hydroclimate proxies. We note that different proxies show variable gradual and abrupt responses to individual hydroclimate events, and thus might have different inherent sensitivities, which may be a factor contributing to the controversy surrounding the abruptness of past events such as the AHP. During the Late Pleistocene the Nile Basin experienced extreme hydroclimate fluctuations, which presumably impacted Paleolithic cultures residing along the Nile corridor.

27 CFR 21.122 - Pyridine bases.

Code of Federal Regulations, 2010 CFR

2010-04-01

... with 1 N H2SO4 until a drop of the mixture placed upon Congo paper shows a distinct blue border, which soon disappears. A minimum of 9.5 ml of the acid must be required for the end point. (Congo paper: filter paper treated with 0.1 percent aqueous solution of Congo red and dried.) (b) Distillation range...

78 FR 16028 - Waiver of Restriction on Assistance to the Central Government of the Democratic Republic of Congo

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-13

... DEPARTMENT OF STATE [Public Notice 8231] Waiver of Restriction on Assistance to the Central Government of the Democratic Republic of Congo Pursuant to Section 7031(b)(3) of the Department of State... the Act with respect to the Democratic Republic of Congo and I hereby waive this restriction. This...

75 FR 77044 - Blocking of Specially Designated National Pursuant to Executive Order 13413

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-10

... Conflict in the Democratic Republic of Congo.'' DATES: The designation by the Director of OFAC of the four... States Code. In the Order, the President found that the situation in the Democratic Republic of the Congo.... DOB 1975; POB Ngungu, Masisi Territory, North Kivu province, Democratic Republic of the Congo; Lt. Col...

27 CFR 21.122 - Pyridine bases.

Code of Federal Regulations, 2012 CFR

2012-04-01

... with 1 N H2SO4 until a drop of the mixture placed upon Congo paper shows a distinct blue border, which soon disappears. A minimum of 9.5 ml of the acid must be required for the end point. (Congo paper: filter paper treated with 0.1 percent aqueous solution of Congo red and dried.) (b) Distillation range...

27 CFR 21.122 - Pyridine bases.

Code of Federal Regulations, 2011 CFR

2011-04-01

... with 1 N H2SO4 until a drop of the mixture placed upon Congo paper shows a distinct blue border, which soon disappears. A minimum of 9.5 ml of the acid must be required for the end point. (Congo paper: filter paper treated with 0.1 percent aqueous solution of Congo red and dried.) (b) Distillation range...

77 FR 65251 - Continuation of the National Emergency With Respect to the Situation in or in Relation to the...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-26

... the Democratic Republic of the Congo On October 27, 2006, by Executive Order 13413, the President... the Congo and, pursuant to the International Emergency Economic Powers Act (50 U.S.C. 1701-1706... the Congo, which has been marked by widespread violence and atrocities that continue to threaten...

27 CFR 21.122 - Pyridine bases.

Code of Federal Regulations, 2014 CFR

2014-04-01

... with 1 N H2SO4 until a drop of the mixture placed upon Congo paper shows a distinct blue border, which soon disappears. A minimum of 9.5 ml of the acid must be required for the end point. (Congo paper: filter paper treated with 0.1 percent aqueous solution of Congo red and dried.) (b) Distillation range...

27 CFR 21.122 - Pyridine bases.

Code of Federal Regulations, 2013 CFR

2013-04-01

... with 1 N H2SO4 until a drop of the mixture placed upon Congo paper shows a distinct blue border, which soon disappears. A minimum of 9.5 ml of the acid must be required for the end point. (Congo paper: filter paper treated with 0.1 percent aqueous solution of Congo red and dried.) (b) Distillation range...

Collaboration between Higher Education and Labor Market in Kinshasa, DR Congo

ERIC Educational Resources Information Center

Etshim, Rachal

2017-01-01

The transition of new graduate students from school to the labor market in Democratic Republic of Congo has been a major topic for debate over the last twenty years. This study identifies the factors affecting collaboration between higher education and the labor market in Kinshasa, the Capital city of the Democratic Republic of Congo. Even though…

Virulent strain of African swine fever virus eclipses its attenuated derivative after challenge.

PubMed

Titov, Ilya; Burmakina, Galina; Morgunov, Yuriy; Morgunov, Sergey; Koltsov, Andrey; Malogolovkin, Alexander; Kolbasov, Denis

2017-10-01

African swine fever (ASF) is one of the most devastating diseases affecting the swine industry worldwide. No effective vaccine is currently available for disease prevention and control. Although live attenuated vaccines (LAV) have demonstrated great potential for immunizing against homologous strains of African swine fever virus (ASFV), adverse reactions from LAV remain a concern. Here, by using a homologous ASFV Congo strain system, we show passage-attenuated Congo LAV to induce an efficient protective immune response against challenge with the virulent parental Congo strain. Notably, only the parental challenge Congo strain was identified in blood and organs of recovered pigs through B602L gene PCR, long-range PCR, nucleotide sequencing and virus isolation. Thus, despite the great protective potential of homologous attenuated ASFV strain, the challenge Congo strain can persist for weeks in recovered pigs and a recrudescence of virulent virus at late time post-challenge may occur.

Classification of very high resolution satellite remote sensing data in a pilot phase of the forest cover classification of the Democratic Republic of Congo, Forêts d'Afrique Central Evaluées par Télédetection (FACET) product

NASA Astrophysics Data System (ADS)

Singa Monga Lowengo, C.

2012-12-01

The Observatoire Satellital des Forêts d'Afrique Centrale (OSFAC) based in Kinshasa, serves as the focal point of the GOFC-GOLD network for Central Africa. OSFAC's long term objective is building regional capacity to use remotely sensed data to map forest cover and forest cover change across Central Africa. OSFAC archives and disseminates satellite data, offers training in geospatial data applications in coordination with the University of Kinshasa, and provides technical support to CARPE partners. Forêts d'Afrique Centrale Évaluées par Télédétection (FACET) is an OSFAC initiative that implements the UMD/SDSU methodology at the national level and quantitatively evaluates the spatiotemporal dynamics of forest cover in Central Africa. The multi-temporal series of FACET data is a useful contribution to many projects, such as biodiversity monitoring, climate modeling, conservation, natural resource management, land use planning, agriculture and REDD+. I am working as Remote Sensing and GIS Officer in various projects of OSFAC. My activities include forest cover and lands dynamics monitoring in Congo Basin. I am familiar with the use of digital mapping software, GIS and RS (Arc GIS, ENVI and PCI Geomatica etc.), classification and spatial Analysis of satellite images, 3D modeling, etc. I started as an intern at OSFAC, Assistant Trainer (Professional Training) and Consultant than permanent employee since October 2009. To assist in the OSFAC activities regarding the monitoring of forest cover and the CARPE program in the context of natural resources management, I participated in the development of the FACET Atlas (Republic of Congo). I received data from Matt Hansen (map.img), WRI and Brazzaville (shapefiles). With all these data I draw maps of the ROC Atlas and statistics of forest cover and forest loss. We organize field work on land to collect data to validate the FACET product. Therefore, to assess forest cover in the region of Kwamouth and Kahuzi-Maiko Biega landscape with very high resolution data and field work for validating FACET product (Remotelly Sensing Product).;

Geochronology and nature of the Palaeoproterozoic basement in the Central African Copperbelt (Zambia and the Democratic Republic of Congo), with regional implications

NASA Astrophysics Data System (ADS)

Rainaud, C.; Master, S.; Armstrong, R. A.; Robb, L. J.

2005-07-01

U-Pb SHRIMP zircon age data, together with geochemical analyses, from the basement to the Katanga Supergroup in the Central African Copperbelt reveal the existence of a widespread Palaeoproterozoic magmatic arc terrane. The Lufubu schists represent a long-lived calc-alkaline volcanic arc sequence and, where dated in both Zambia and the Democratic Republic of Congo (DRC), yield ages of 1980 ± 7, 1968 ± 9, 1964 ± 12 and 1874 ± 8 Ma. The oldest dated unit from the region, the Mkushi granitic gneiss from south-east of the Zambian Copperbelt, has an age of 2049 ± 6 Ma. The copper-mineralized Mtuga aplites, which crosscut the foliation in the Mkushi gneisses, have mainly xenocrystic, zoned zircons with cores dated at ca. 2.07-2.00 Ga. Overgrowths on these cores are dated at 1059 ± 26 Ma, which is interpreted as the intrusive age of the aplites. An augen gneiss from the Mulungushi Bridge locality yielded an emplacement age of 1976 ± 5 Ma. The Mufulira Pink Granite has an age of 1994 ± 7 Ma, while the Chambishi granite has been dated at 1983 ± 5 Ma, an age within error of Lufubu schist metavolcanics from elsewhere in the Chambishi basin. The gneisses, granitoids and acid-intermediate calc-alkaline metavolcanics are considered to represent stages in the evolution of one or more magmatic arcs that formed episodically over a 200 million year period between 2050 and 1850 Ma. We suggest naming this assemblage of rocks the "Lufubu Metamorphic Complex". The rocks of the Lufubu Metamorphic Complex are interpreted to be part of a regionally extensive Palaeoproterozoic magmatic arc terrane stretching from northern Namibia to northern Zambia and the DRC. This terrane is termed the Kamanjab-Bangweulu arc and is inferred to have collided with the Archaean Tanzanian craton during the ca. 2.0-1.9 Ga Ubendian orogeny, to produce a new composite minicontinental entity that we term the "Kambantan" terrane. The Kambantan terrane was accreted onto the southern margin of the Congo craton during the ca. 1.4-1.0 Kibaran orogeny.

The Ritual "Play of the Congos" of North-Central Panama: Its Sociolinguistic Implications. Sociolinguistic Working Paper Number 85.

ERIC Educational Resources Information Center

Joly, Luz Graciela

An excerpt from the "Play of the Congos," given in Congo, Spanish, and English, exemplifies the sociolinguistic features of the combined play and ritual language used by the Afro-Hispanic population in the Caribbean region of the Costa Abajo in north-central Panama. The sociolinguistic norms are an important part of the "regulation…

3 CFR - Continuation of the National Emergency With Respect to the Situation in or in Relation to the...

Code of Federal Regulations, 2011 CFR

2011-01-01

... to the Situation in or in Relation to the Democratic Republic of the Congo Presidential Documents... Respect to the Situation in or in Relation to the Democratic Republic of the Congo On October 27, 2006, by... relation to the Democratic Republic of the Congo and, pursuant to the International Emergency Economic...

78 FR 76699 - Waiver of Restriction on Assistance to the Central Government of the Republic of the Congo

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-18

... DEPARTMENT OF STATE [Public Notice 8551] Waiver of Restriction on Assistance to the Central Government of the Republic of the Congo Pursuant to Section 7031(b)(3) of the Department of State, Foreign... prior year Acts with respect to the Republic of the Congo and I hereby waive this restriction. This...

3 CFR - Continuation of the National Emergency With Respect to the Situation in or in Relation to the...

Code of Federal Regulations, 2012 CFR

2012-01-01

... to the Situation in or in Relation to the Democratic Republic of the Congo Presidential Documents... Respect to the Situation in or in Relation to the Democratic Republic of the Congo On October 27, 2006, by... relation to the Democratic Republic of the Congo and, pursuant to the International Emergency Economic...

3 CFR - Continuation of the National Emergency With Respect to the Situation in or in Relation to the...

Code of Federal Regulations, 2013 CFR

2013-01-01

... to the Situation in or in Relation to the Democratic Republic of the Congo Presidential Documents... Respect to the Situation in or in Relation to the Democratic Republic of the Congo On October 27, 2006, by... relation to the Democratic Republic of the Congo and, pursuant to the International Emergency Economic...

3 CFR - Continuation of the National Emergency With Respect to the Situation in or in Relation to the...

Code of Federal Regulations, 2014 CFR

2014-01-01

... to the Situation in or in Relation to the Democratic Republic of the Congo Presidential Documents... Respect to the Situation in or in Relation to the Democratic Republic of the Congo On October 27, 2006, by... relation to the Democratic Republic of the Congo and, pursuant to the International Emergency Economic...

Outbreak of cholera in the Republic of Congo and the Democratic Republic of Congo, and cholera worldwide.

PubMed

Kelvin, Alyson Ann

2011-10-13

Cholera is an acute intestinal disease caused by infection of the Vibrio cholerae bacterium.  Often manifested as a constant diarrhoeal disease, Cholera is associated with significant mortality as well as economic loss due to the strain on health care.  Cholera often affects nations with lower economic status.  The recent outbreak of cholera in the Republic of Congo and the Democratic Republic of Congo has affected thousands of people.  Here we review the past cholera epidemiology, molecular mechanisms of the bacterium, and the political and environmental aspects that affect the treatment and eradication of this disease.

The effect of the 2011 flood on agricultural chemical and sediment movement in the lower Mississippi River Basin

NASA Astrophysics Data System (ADS)

Welch, H.; Coupe, R.; Aulenbach, B.

2012-04-01

Extreme hydrologic events, such as floods, can overwhelm a surface water system's ability to process chemicals and can move large amounts of material downstream to larger surface water bodies. The Mississippi River is the 3rd largest River in the world behind the Amazon in South America and the Congo in Africa. The Mississippi-Atchafalaya River basin grows much of the country's corn, soybean, rice, cotton, pigs, and chickens. This is large-scale modern day agriculture with large inputs of nutrients to increase yields and large applied amounts of crop protection chemicals, such as pesticides. The basin drains approximately 41% of the conterminous United States and is the largest contributor of nutrients to the Gulf of Mexico each spring. The amount of water and nutrients discharged from the Mississippi River has been related to the size of the low dissolved oxygen area that forms off of the coast of Louisiana and Texas each summer. From March through April 2011, the upper Mississippi River basin received more than five times more precipitation than normal, which combined with snow melt from the Missouri River basin, created a historic flood event that lasted from April through July. The U.S. Geological Survey, as part of the National Stream Quality Accounting Network (NASQAN), collected samples from six sites located in the lower Mississippi-Atchafalaya River basin, as well as, samples from the three flow-diversion structures or floodways: the Birds Point-New Madrid in Missouri and the Morganza and Bonnet Carré in Louisiana, from April through July. Samples were analyzed for nutrients, pesticides, suspended sediments, and particle size; results were used to determine the water quality of the river during the 2011 flood. Monthly loads for nitrate, phosphorus, pesticides (atrazine, glyphosate, fluometuron, and metolachlor), and sediment were calculated to quantify the movement of agricultural chemicals and sediment into the Gulf of Mexico. Nutrient loads were compared to historic loads to assess the effect of the flood on the zone of hypoxia that formed in the Gulf of Mexico during the spring of 2011.

Mycoremediation of congo red dye by filamentous fungi.

PubMed

Bhattacharya, Sourav; Das, Arijit; G, Mangai; K, Vignesh; J, Sangeetha

2011-10-01

Azo, anthroquinone and triphenylmethane dyes are the major classes of synthetic colourants, which are difficult to degrade and have received considerable attention. Congo red, a diazo dye, is considered as a xenobiotic compound, and is recalcitrant to biodegradative processes. Nevertheless, during the last few years it has been demonstrated that several fungi, under certain environmental conditions, are able to transfer azo dyes to non toxic products using laccases. The aim of this work was to study the factors influencing mycoremediation of Congo red. Several basidiomycetes and deuteromycetes species were tested for the decolourisation of Congo red (0.05 g/l) in a semi synthetic broth at static and shaking conditions. Poor decolourisation was observed when the dye acted as the sole source of nitrogen, whereas semi synthetic broth supplemented with fertilizer resulted in better decolourisation. Decolourisation of Congo red was checked in the presence of salts of heavy metals such as mercuric chloride, lead acetate and zinc sulphate. Decolourisation parameters such as temperature, pH, and rpm were optimized and the decolourisation obtained at optimized conditions varied between 29.25- 97.28% at static condition and 82.1- 100% at shaking condition. Sodium dodecyl sulphate polyacrylamide gel electrophoretic analysis revealed bands with molecular weights ranging between 66.5 to 71 kDa, a characteristic of the fungal laccases. High efficiency decolourisation of Congo red makes these fungal forms a promising choice in biological treatment of waste water containing Congo red.

Time and Origin of Cichlid Colonization of the Lower Congo Rapids

PubMed Central

Schwarzer, Julia; Misof, Bernhard; Ifuta, Seraphin N.; Schliewen, Ulrich K.

2011-01-01

Most freshwater diversity is arguably located in networks of rivers and streams, but, in contrast to lacustrine systems riverine radiations, are largely understudied. The extensive rapids of the lower Congo River is one of the few river stretches inhabited by a locally endemic cichlid species flock as well as several species pairs, for which we provide evidence that they have radiated in situ. We use more that 2,000 AFLP markers as well as multilocus sequence datasets to reconstruct their origin, phylogenetic history, as well as the timing of colonization and speciation of two Lower Congo cichlid genera, Steatocranus and Nanochromis. Based on a representative taxon sampling and well resolved phylogenetic hypotheses we demonstrate that a high level of riverine diversity originated in the lower Congo within about 5 mya, which is concordant with age estimates for the hydrological origin of the modern lower Congo River. A spatial genetic structure is present in all widely distributed lineages corresponding to a trisection of the lower Congo River into major biogeographic areas, each with locally endemic species assemblages. With the present study, we provide a phylogenetic framework for a complex system that may serve as a link between African riverine cichlid diversity and the megadiverse cichlid radiations of the East African lakes. Beyond this we give for the first time a biologically estimated age for the origin of the lower Congo River rapids, one of the most extreme freshwater habitats on earth. PMID:21799840

Glycosaminoglycans mediate retention of the poxvirus type I interferon binding protein at the cell surface to locally block interferon antiviral responses

PubMed Central

Montanuy, Imma; Alejo, Ali; Alcami, Antonio

2011-01-01

Eradication of smallpox was accomplished 30 yr ago, but poxviral infections still represent a public health concern due to the potential release of variola virus or the emergence of zoonotic poxviruses, such as monkeypox virus. A critical determinant of poxvirus virulence is the inhibition of interferons (IFNs) by the virus-encoded type I IFN-binding protein (IFNα/βBP). This immunomodulatory protein is secreted and has the unique property of interacting with the cell surface in order to prevent IFN-mediated antiviral responses. However, the mechanism of its attachment to the cell surface remains unknown. Using surface plasmon resonance and cell-binding assays, we report that the IFNα/βBP from vaccinia virus, the smallpox vaccine, interacts with cell surface glycosaminoglycans (GAGs). Analysis of the contribution of different regions of the protein to cell surface binding demonstrated that clusters of basic residues in the first immunoglobulin domain mediate GAG interactions. Furthermore, mutation of the GAG-interaction motifs does not affect its IFN-binding and -blocking capacity. Functional conservation of GAG-binding sites is demonstrated for the IFNα/βBP from variola and monkeypox viruses, extending our understanding of immune modulation by the most virulent human poxviruses. These results are relevant for the design of improved vaccines and intervention strategies.—Montanuy, I., Alejo, A., Alcami, A. Glycosaminoglycans mediate retention of the poxvirus type I interferon binding protein at the cell surface to locally block interferon antiviral responses. PMID:21372110

Variola and Monkeypox Viruses Utilize Conserved Mechanisms of Virion Motility and Release That Depend on Abl and Src Family Tyrosine Kinases▿ †

PubMed Central

Reeves, Patrick M.; Smith, Scott K.; Olson, Victoria A.; Thorne, Steve H.; Bornmann, William; Damon, Inger K.; Kalman, Daniel

2011-01-01

Vaccinia virus (VacV) enters mammalian cells, replicates extranuclearly, and produces virions that move to the cell surface along microtubules, fuse with the plasma membrane, and move from infected cells toward apposing cells on actin-filled membranous protrusions or actin tails. To form actin tails, cell-associated enveloped virions (CEV) require Abl and Src family tyrosine kinases. Furthermore, release of CEV from the cell requires Abl but not Src family tyrosine kinases and is blocked by imatinib mesylate (STI-571; Gleevec), an Abl family kinase inhibitor used to treat chronic myelogenous leukemia in humans. Here we demonstrate that the Poxviridae family members monkeypox virus (MPX) and variola virus (VarV) use conserved mechanisms for actin motility and extracellular enveloped virion (EEV) release. Furthermore, we show that imatinib mesylate is effective in a mouse model of infection with VacV, whether delivered prophylactically or postinfection, and restricts spread of virions from the site of inoculation. While inhibitors of both Src and Abl family kinases, such as dasatinib (BMS-354825; Sprycel), are effective in limiting dissemination of VacV, VarV, and MPX in vitro, members of this class of drugs appear to have immunosuppressive effects in vivo that preclude their use as anti-infectives. Together, these data suggest a possible utility for imatinib mesylate in treating smallpox or MPX infections or complications associated with vaccination. PMID:20962097

Analysis of the Soviet Crisis Management Experience

DTIC Science & Technology

1978-09-30

between Congo (Zaire) and Portugual ; Congo charges that Tshombe opposition forces are . operating out of Portuguese Cabinda; Portugal charges that Congo has...regime (for example, South Vietnam, Rhodesia, Portuguese colonies in Africa), or denial of military access (that is, Western and Chinese). * The USSR was...172 601118 French paratroops intervene to aid pro-French regime in Gabon. *• 173 610315- The Soviet Union opposes continued Portuguese colonial

A STAT-1 Knockout Mouse Model for Machupo Virus Pathogenesis

DTIC Science & Technology

2011-06-14

hemorrhagic fever viruses, including Ebola, Marburg, Junín, and Crimean - Congo Hemorrhagic Fever viruses [11-14...Akerstrom S, Klingstrom J, Mirazimi A: Crimean - Congo hemorrhagic fever virus infection is lethal for adult type I interferon receptor-knockout mice. J...Shieh WJ, Camus G, Stroher U, Zaki S, Jones SM: Pathogenesis and immune response of Crimean - Congo hemorrhagic fever virus in a STAT-1 knockout

MassTag Polymerase Chain Reaction for Differential Diagnosis of Viral Hemorrhagic Fevers

DTIC Science & Technology

2006-04-01

fever virus (RVFV), Crimean - Congo hemorrhagic fever virus (CCHFV), and hantaviruses (Bunyaviridae); and...ribavirin may be helpful if given early in the course of Lassa fever (9), Crimean - Congo hemorrhagic fever (10), or hemorrhagic fever with renal...I, Erol S, Erdem F, Yilmaz N, Parlak M, et al. Crimean - Congo hemorrhagic fever in eastern Turkey: clinical fea- tures, risk factors and efficacy

75 FR 65933 - Continuation of the National Emergency With Respect to the Situation in or in Relation to the...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-26

... Respect to the Situation in or in Relation to the Democratic Republic of the Congo #0; #0; #0... Republic of the Congo On October 27, 2006, by Executive Order 13413, the President declared a national emergency with respect to the situation in or in relation to the Democratic Republic of the Congo and...

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Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-26

... Republic of the Congo #0; #0; #0; Presidential Documents #0; #0; #0;#0;Federal Register / Vol. 76, No. 207... Relation to the Democratic Republic of the Congo On October 27, 2006, by Executive Order 13413, the... Republic of the Congo and, pursuant to the International Emergency Economic Powers Act (50 U.S.C. 1701-1706...

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Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-25

... Republic of the Congo #0; #0; #0; Presidential Documents #0; #0; #0;#0;Federal Register / Vol. 78 , No. 207... Relation to the Democratic Republic of the Congo On October 27, 2006, by Executive Order 13413, the... Republic of the Congo and, pursuant to the International Emergency Economic Powers Act (50 U.S.C. 1701-1706...

Congo Red Stain Identifies Matrix Overproduction and Is an Indirect Measurement for c-di-GMP in Many Species of Bacteria.

PubMed

Jones, Christopher J; Wozniak, Daniel J

2017-01-01

Congo red is a diazo textile dye that has been used to visualize the production of amyloid fibers for nearly a century. Microbiological applications were later developed, especially in identifying strains that produce amyloid appendages called curli and overexpressing polysaccharides in the biofilm matrix. The second messenger cyclic diguanylate (c-di-GMP) regulates the production of biofilm matrix polysaccharides, and therefore Congo red staining of samples can be utilized as an indirect measurement of elevated c-di-GMP production in bacteria. Congo red allows the identification of strains producing high c-di-GMP in an inexpensive, quantitative, and high-throughput manner.

Discharge and other hydraulic measurements for characterizing the hydraulics of Lower Congo River

USGS Publications Warehouse

Oberg, Kevin; Shelton, John M.; Gardiner, Ned; Jackson, P. Ryan

2009-01-01

The first direct measurements of discharge of the Lower Congo River below Malebo Pool and upstream from Kinganga, Democratic Republic of Congo (DRC) were made in July 2008 using acoustic Doppler current profilers, differential GPS, and echo sounders. These measurements were made in support of research that is attempting to understand the distribution of fish species in the Lower Congo River and reasons for separation of species within this large river. Analyses of these measurements show that the maximum depth in the Lower Congo River was in excess of 200 m and maximum water velocities were greater than 4 m/s. The discharge measured near Luozi, DRC was 35,800 m3/s, and decreased slightly beginning midway through the study. Local bedrock controls seem to have a large effect on the flow in the river, even in reaches without waterfalls and rapids. Dramatic changes in bed topography are evident in transects across the river.

Physiological and biochemical responses of Chlorella vulgaris to Congo red.

PubMed

Hernández-Zamora, Miriam; Perales-Vela, Hugo Virgilio; Flores-Ortíz, César Mateo; Cañizares-Villanueva, Rosa Olivia

2014-10-01

Extensive use of synthetic dyes in many industrial applications releases large volumes of wastewater. Wastewaters from dying industries are considered hazardous and require careful treatment prior to discharge into receiving water bodies. Dyes can affect photosynthetic activities of aquatic flora and decrease dissolved oxygen in water. The aim of this study was to evaluate the effect of Congo red on growth and metabolic activity of Chlorella vulgaris after 96h exposure. Exposure of the microalga to Congo red reduced growth rate, photosynthesis and respiration. Analysis of chlorophyll a fluorescence emission showed that the donor side of photosystem II was affected at high concentrations of Congo red. The quantum yield for electron transport (φEo), the electron transport rate (ETR) and the performance index (PI) also decreased. The reduction in the ability to absorb and use the quantum energy increased non-photochemical (NPQ) mechanisms for thermal dissipation. Overall, Congo red affects growth and metabolic activity in photosynthetic organisms in aquatic environments. Copyright © 2014 Elsevier Inc. All rights reserved.

Decolorization of Congo Red by Phanerochaete chrysosporium: the role of biosorption and biodegradation.

PubMed

Bosco, Francesca; Mollea, Chiara; Ruggeri, Bernardo

2017-10-01

The degradation of Congo Red by means of Phanerochaete chrysosporium BKM-F-1767 is reported in this work. Solid and liquid cultures have been prepared to evaluate in vivo biodegradation as well as the role of biosorption phenomena on mycelium. Moreover, in vitro tests have been performed to define the influence of MnP on dye decolorization. P. chrysosporium, cultivated on Malt Extract Agar in the presence of Congo Red 0.005% (w/v), has shown good growth and the ability to decolorize the dye in the 25-39°C temperature range. It has also been cultivated in a low NMM liquid medium with the aforementioned dye concentration in immobilized stationary cultures inducted for Lignin Peroxidase (LiP) and Manganese Peroxidase (MnP) production. Congo Red was absorbed on the biomass and then decolorized (93% and 85% for the LiP and MnP cultures, respectively). The cultures with added Congo Red have shown a higher MnP synthesis rate than a control without the dye. The enzymatic degradation of Congo Red has also been investigated by means of the extracellular fluid for different MnP activities (0-300 IU/l); the decolorization percentage has been found to be clearly related to the enzyme concentration up to a value of about 200 IU/l.

THE GEOLOGIC RISK IN THE LAKE KIVU BASIN AREA PRODUCTED BY EARTHQUAKES. Case of the February 3th 2008 earthquake. By: L.M.Bagalwa(1), F.Lukaya(1), M.Burume(2), J.Moeyerson(3) (1): Goma Volcano Observatory, D.R.Congo (2): Naturals Sciences Research Center

NASA Astrophysics Data System (ADS)

Bagalwa Rukeza, Montfort

2010-05-01

The eastern Democratic Republic of Congo is prone to earthquakes of magnitude greater than or equal to 4 on the Richter scale. The western edge of Lake Kivu, the most populated part of the region is no exception to the solicitation of these earthquakes. Since 1997, the western basin of Lake Kivu is experiencing intense seismicity, several earthquakes of great intensity, magnitude greater than or equal to 4 develop major destructive phenomena. These include the 1997 earthquake (M = 4.7) 2000 (M = 4.6 and 5.4), 2002 (M = 4.9, 5.2, 6.1 and 24 October 2002 M = 6.2) of February 3rd 2008 (M = 6). Earthquakes of Kalehe on October 24th 2002 and Birava, February 3rd 2008 have resulted deformations of soil, human and material damage. This latest natural disaster ever known in the south-western basin of Lake Kivu has attracted our scientific curiosity we go there to inquire into its causes and consequences in this region. The basin of Lake Kivu is affected by transform faults emerging (MUKONKI & CHOROWICZ, 1980, quoted by K.S.KAVOTHA & ali, 1990) that delimit the Rift were intersecting at the level of Lake Kivu. We Consider the seismicity, volcanism and uplift of the basin of Lake Kivu as a sign of fracturing under way to delimit a plate tectonics formed (Wong and Von Herzen, 1974, quoted by KSKAVOTHA et al, 1990). The physiography of Lake Kivu is dominated by the fault which borders the western shore and one which intersects the island of Idjwi. The telemetry data of Goma Volcano Observatory added to those of the seismographic station of Lwiro have always revealed a pattern of epicenters clearer in Lake Kivu. In correlation with the faults of the region, earthquakes affect mainly the western edge of Lake Kivu and the island of Idjwi with increasing density from north to south (K.S.KAVOTHA et al, 1990). The great earthquake of Lake Kivu basin on February 03rd 2008, of magnitude 6 on the Richter scale occurred at 07 hours 34 minutes 12 seconds GMT, about 20 km north of Bukavu, 80km south-west of Goma, between 02,314S and 028,896E, at a depth of 10km epicentral surface. Three major aftershocks followed to this great earthquake and were recorded at the seismographic station of Lwiro and the Goma Volcano Observatory: 1. The first after shock at 10 hours 56minutes 10seconds AM GMT, of magnitude 5.0, located at 20km depth and oriented on the north-east of Bukavu and 80km depth on the west of Butare in Rwanda between 02.456 S and 029.039 E. 2. The second after shock at 11 hours 07 minutes AM GMT, of magnitude 4.7, located at 25km depth on the north-east of Bukavu and 75 km depth on the south-west of Goma, between 02.307S and 028.997E. 3. The third after shock at 11hours 37minutes 49secondes AM GMT, of magnitude 4.5, located at 40km depth on the west of Butare in Rwanda and 55km depth on the east of Bukavu between 02.525S and 029.363E (Lukaya N'yombo Fr,11 February 2008). Other after shocks not indicated in this text was shacked the western of Lake Kivu basin. This great earthquake and its first two aftershocks were located in the south western of Lake Kivu basin, in Ishungu and Birava region in territory of Kabare. The damage is observed on a radius of approximately 20 km. This earthquake has reactivated the faults along the western shore of the Lake Kivu, but also those of the Fomulac-Kakondo-Ishungu-Birava axis. Those in Bukavu town, the South and North direction have not escaped at this reactivation. The movement of these faults has caused deformations in the surface soil and buildings erected on these flaws have suffered cracks and destruction. We will present damages of this natural risk in our poster presentation.

The bonobo-dialium positive interactions: seed dispersal mutualism.

PubMed

Beaune, David; Bretagnolle, François; Bollache, Loïc; Hohmann, Gottfried; Surbeck, Martin; Bourson, Chloé; Fruth, Barbara

2013-04-01

A positive interaction is any interaction between individuals of the same or different species (mutualism) that provides a benefit to both partners such as increased fitness. Here we focus on seed dispersal mutualism between an animal (bonobo, Pan paniscus) and a plant (velvet tamarind trees, Dialium spp.). In the LuiKotale rainforest southwest of Salonga National Park, Democratic Republic of Congo, seven species of the genus Dialium account for 29.3% of all trees. Dialium is thus the dominant genus in this forest. Dialium fruits make up a large proportion of the diet of a habituated bonobo community in this forest. During the 6 months of the fruiting season, more than half of the bonobos' feeding time is devoted to Dialium fruits. Furthermore, Dialium fruits contribute a considerable proportion of sugar and protein to bonobos' dietary intake, being among the richest fruits for these nutrients. Bonobos in turn ingest fruits with seeds that are disseminated in their feces (endozoochory) at considerable distances (average: 1.25 km after 24 hr of average transit time). Endozoochory through the gut causes loss of the cuticle protection and tegumentary dormancy, as well as an increase in size by water uptake. Thus, after gut passage, seeds are better able to germinate. We consider other primate species as a potential seed disperser and conclude that Dialium germination is dependent on passage through bonobo guts. This plant-animal interaction highlights positive effects between two major organisms of the Congo basin rainforest, and establishes the role of the bonobo as an efficient disperser of Dialium seeds. Periodicals, Inc. © 2013 Wiley Periodicals, Inc.

Knowledge Gained and Retained from a Video-Centered, Community-Based Intervention for Ebola Prevention, Congo.

PubMed

Roess, Amira A; Di Peppi, Rosemarie; Kinzoni, Eric A; Molouania, Michaelle; Kennedy, Emily; Ibata, Saturnin R; Badinga, Nkenda; Mabola, Fredy S; Moses, Cynthia

2017-11-01

There are few effective communication interventions to respond to Ebola Virus Disease (EVD) outbreaks, and their development is a continuing challenge. The largest outbreak of EVD was first recognized in 2014 and once again highlighted the need for health education communication campaigns. EVD emergence and transmission are associated with behaviors that increase exposure to infected individuals and infected wildlife. In 2008-2009, an educational video-centered intervention consisting of a film followed by a structured discussion was developed to improve EVD knowledge, attitudes, and behaviors (KABs) for prevention of EVD and care of infected people. The film was produced for a Congolese audience in local languages and features doctors and residents from the region. The objective of the study was to assess the effectiveness of this intervention to change KABs related to EVD. The intervention was conducted over 12 months and reached >60,000 people; 220 individuals were sampled before and immediately after the intervention, and a subsample of 53 who had received the intervention 1 year earlier were interviewed to assess changes in KABs related to EVD. Statistically significant improvements in EVD recognition, transmission, and mitigation of risk after the intervention were observed. This study also demonstrates significant retention of knowledge one year after the intervention. Overall, the intervention was effective in improving KABs among a limited literacy population and knowledge was retained for more than one year. The materials and approaches developed were used in 2014 as part of a social mobilization strategy in response to an Ebola outbreak in the Congo Basin.

Immunization Coverage

MedlinePlus

... Pacific WHO in countries » Overview Statistics Cooperation strategies Democratic Republic of the Congo » Emergencies Focus on » Bangladesh Rohingya Democratic Republic of the Congo Iraq Nigeria Somalia South ...

Optical properties of amyloid stained by Congo red: history and mechanisms.

PubMed

Howie, Alexander J; Brewer, Douglas B

2009-04-01

Amyloid stained by Congo red has striking optical properties that generally have been poorly described and inadequately explained, although they can be understood from principles of physical optics. Molecules of Congo red are orientated on amyloid fibrils, and so the dye becomes dichroic and birefringent. The birefringence varies with wavelength in accordance with a fundamental property of all light-transmitting materials called anomalous dispersion of the refractive index around an absorption peak. The combination of this and absorption of light, with modification by any additional birefringence in the optical system, explains the various colours that can be seen in Congo red-stained amyloid between crossed polariser and analyser, and also when the polariser and analyser are progressively uncrossed. These are called anomalous colours.

Modulating Vaccinia Virus Immunomodulators to Improve Immunological Memory

PubMed Central

Torres, Alice A.; Smith, Geoffrey L.

2018-01-01

The increasing frequency of monkeypox virus infections, new outbreaks of other zoonotic orthopoxviruses and concern about the re-emergence of smallpox have prompted research into developing antiviral drugs and better vaccines against these viruses. This article considers the genetic engineering of vaccinia virus (VACV) to enhance vaccine immunogenicity and safety. The virulence, immunogenicity and protective efficacy of VACV strains engineered to lack specific immunomodulatory or host range proteins are described. The ultimate goal is to develop safer and more immunogenic VACV vaccines that induce long-lasting immunological memory. PMID:29495547

Multicriteria evaluation of discharge simulation in Dynamic Global Vegetation Models

NASA Astrophysics Data System (ADS)

Yang, Hui; Piao, Shilong; Zeng, Zhenzhong; Ciais, Philippe; Yin, Yi; Friedlingstein, Pierre; Sitch, Stephen; Ahlström, Anders; Guimberteau, Matthieu; Huntingford, Chris; Levis, Sam; Levy, Peter E.; Huang, Mengtian; Li, Yue; Li, Xiran; Lomas, Mark R.; Peylin, Philippe; Poulter, Ben; Viovy, Nicolas; Zaehle, Soenke; Zeng, Ning; Zhao, Fang; Wang, Lei

2015-08-01

In this study, we assessed the performance of discharge simulations by coupling the runoff from seven Dynamic Global Vegetation Models (DGVMs; LPJ, ORCHIDEE, Sheffield-DGVM, TRIFFID, LPJ-GUESS, CLM4CN, and OCN) to one river routing model for 16 large river basins. The results show that the seasonal cycle of river discharge is generally modeled well in the low and middle latitudes but not in the high latitudes, where the peak discharge (due to snow and ice melting) is underestimated. For the annual mean discharge, the DGVMs chained with the routing model show an underestimation. Furthermore, the 30 year trend of discharge is also underestimated. For the interannual variability of discharge, a skill score based on overlapping of probability density functions (PDFs) suggests that most models correctly reproduce the observed variability (correlation coefficient higher than 0.5; i.e., models account for 50% of observed interannual variability) except for the Lena, Yenisei, Yukon, and the Congo river basins. In addition, we compared the simulated runoff from different simulations where models were forced with either fixed or varying land use. This suggests that both seasonal and annual mean runoff has been little affected by land use change but that the trend itself of runoff is sensitive to land use change. None of the models when considered individually show significantly better performances than any other and in all basins. This suggests that based on current modeling capability, a regional-weighted average of multimodel ensemble projections might be appropriate to reduce the bias in future projection of global river discharge.

Multi-criteria Evaluation of Discharge Simulation in Dynamic Global Vegetation Models

NASA Astrophysics Data System (ADS)

Yang, H.; Piao, S.; Zeng, Z.; Ciais, P.; Yin, Y.; Friedlingstein, P.; Sitch, S.; Ahlström, A.; Guimberteau, M.; Huntingford, C.; Levis, S.; Levy, P. E.; Huang, M.; Li, Y.; Li, X.; Lomas, M.; Peylin, P. P.; Poulter, B.; Viovy, N.; Zaehle, S.; Zeng, N.; Zhao, F.; Wang, L.

2015-12-01

In this study, we assessed the performance of discharge simulations by coupling the runoff from seven Dynamic Global Vegetation Models (DGVMs; LPJ, ORCHIDEE, Sheffield-DGVM, TRIFFID, LPJ-GUESS, CLM4CN, and OCN) to one river routing model for 16 large river basins. The results show that the seasonal cycle of river discharge is generally modelled well in the low and mid latitudes, but not in the high latitudes, where the peak discharge (due to snow and ice melting) is underestimated. For the annual mean discharge, the DGVMs chained with the routing model show an underestimation. Furthermore the 30-year trend of discharge is also under-estimated. For the inter-annual variability of discharge, a skill score based on overlapping of probability density functions (PDFs) suggests that most models correctly reproduce the observed variability (correlation coefficient higher than 0.5; i.e. models account for 50% of observed inter-annual variability) except for the Lena, Yenisei, Yukon, and the Congo river basins. In addition, we compared the simulated runoff from different simulations where models were forced with either fixed or varying land use. This suggests that both seasonal and annual mean runoff has been little affected by land use change, but that the trend itself of runoff is sensitive to land use change. None of the models when considered individually show significantly better performances than any other and in all basins. This suggests that based on current modelling capability, a regional-weighted average of multi-model ensemble projections might be appropriate to reduce the bias in future projection of global river discharge.

Reconciling certification and intact forest landscape conservation.

PubMed

Kleinschroth, Fritz; Garcia, Claude; Ghazoul, Jaboury

2018-05-29

In 2014, the Forest Stewardship Council (FSC) added a new criterion to its principles that requires protection of intact forest landscapes (IFLs). An IFL is an extensive area of forest that lacks roads and other signs of human activity as detected through remote sensing. In the Congo basin, our analysis of road networks in formally approved concessionary logging areas revealed greater loss of IFL in certified than in noncertified concessions. In areas of informal (i.e., nonregulated) extraction, road networks are known to be less detectable by remote sensing. Under the current definition of IFL, companies certified under FSC standards are likely to be penalized relative to the noncertified as well as the informal logging sector on account of their planned road networks, despite an otherwise better standard of forest management. This could ultimately undermine certification and its wider adoption, with implications for the future of sustainable forest management.

Microwave hydrology: A trilogy

NASA Technical Reports Server (NTRS)

Stacey, J. M.; Johnston, E. J.; Girard, M. A.; Regusters, H. A.

1985-01-01

Microwave hydrology, as the term in construed in this trilogy, deals with the investigation of important hydrological features on the Earth's surface as they are remotely, and passively, sensed by orbiting microwave receivers. Microwave wavelengths penetrate clouds, foliage, ground cover, and soil, in varying degrees, and reveal the occurrence of standing liquid water on and beneath the surface. The manifestation of liquid water appearing on or near the surface is reported by a microwave receiver as a signal with a low flux level, or, equivalently, a cold temperature. Actually, the surface of the liquid water reflects the low flux level from the cosmic background into the input terminals of the receiver. This trilogy describes and shows by microwave flux images: the hydrological features that sustain Lake Baykal as an extraordinary freshwater resource; manifestations of subsurface water in Iran; and the major water features of the Congo Basin, a rain forest.

Social learning among Congo Basin hunter–gatherers

PubMed Central

Hewlett, Barry S.; Fouts, Hillary N.; Boyette, Adam H.; Hewlett, Bonnie L.

2011-01-01

This paper explores childhood social learning among Aka and Bofi hunter–gatherers in Central Africa. Existing literature suggests that hunter–gatherer social learning is primarily vertical (parent-to-child) and that teaching is rare. We use behavioural observations, open-ended and semi-structured interviews, and informal and anecdotal observations to examine the modes (e.g. vertical versus horizontal/oblique) and processes (e.g. teaching versus observation and imitation) of cultural transmission. Cultural and demographic contexts of social learning associated with the modes and processes of cultural transmission are described. Hunter–gatherer social learning occurred early, was relatively rapid, primarily vertical under age 5 and oblique and horizontal between the ages of 6 and 12. Pedagogy and other forms of teaching existed as early as 12 months of age, but were relatively infrequent by comparison to other processes of social learning such as observation and imitation. PMID:21357239

Origins, seasonality, and fluxes of organic matter in the Congo River

NASA Astrophysics Data System (ADS)

Spencer, Robert G. M.; Hernes, Peter J.; Dinga, Bienvenu; Wabakanghanzi, Jose N.; Drake, Travis W.; Six, Johan

2016-07-01

The Congo River in central Africa represents a major source of organic matter (OM) to the Atlantic Ocean. This study examined elemental (%OC, %N, and C:N), stable isotopic (δ13C and δ15N), and biomarker composition (lignin phenols) of particulate OM (POM) and dissolved OM (DOM) across the seasonal hydrograph. Even though the Congo exhibits an extremely stable intra-annual discharge regime, seasonal variability in OM composition was evident. DOM appears predominantly derived from vascular plant inputs with greater relative contribution during the rising limb and peak in discharge associated with the major November-December discharge maximum. Generally, POM appears to be sourced from soil-derived mineral-associated OM (low C:N, low Λ8, and higher (Ad:Al)v) but the relative proportion of fresh vascular plant material (higher C:N, higher Λ8, and lower (Ad:Al)v) increases with higher discharge. During the study period (September 2009 to November 2010) the Congo exported 29.21 Tg yr-1 of total suspended sediment (TSS), 1.96 Tg yr-1 of particulate organic carbon (POC), and 12.48 Tg yr-1 of dissolved organic carbon. The Congo exports an order of magnitude lower TSS load in comparison to other major riverine sources of TSS (e.g., Ganges and Brahmaputra), but due to its OM-rich character it actually exports a comparable amount of POC. The Congo is also 2.5 times more efficient at exporting dissolved lignin per unit volume compared to the Amazon. Including Congo dissolved lignin data in residence time calculations for lignin in the Atlantic Ocean results in an approximately 10% reduction from the existing estimate, suggesting that this material is more reactive than previously thought.

The role of the heat shock protein Hsp12p in the dynamic response of Saccharomyces cerevisiae to the addition of Congo red.

PubMed

Shamrock, Vanessa J; Duval, Jérôme F L; Lindsey, George G; Gaboriaud, Fabien

2009-05-01

In this study, we investigate the electrohydrodynamic and nanomechanical characteristics of two Saccharomyces cerevisiae yeast strains, a wild-type (WT) strain and a strain overexpressing (OE) Hsp12p, in the presence and absence of hydrophobic Congo red compound. By combining these two advanced biophysical methods, we demonstrate that Hsp12p proteins are mostly located within a thin layer (c. 10 nm thick) positioned at the external side of the cell wall. However, this Hsp12p-enriched layer does not prevent Congo red from entering the cell wall and from interacting with the chitin therein. The entrance of Congo red within the cell wall is reflected in an increase of the turgor pressure for the OE strain and a decrease of that for the WT strain. It is shown that these opposite trends are consistent with significant modulations of the water content within the cell wall from/to the cytoplasm. These are the result of changes in the hydrophobicity/hydrophilicity balance, as governed by the intertwined local concentration variations of Congo red and Hsp12p across the cell wall. In particular, the decrease of the turgor pressure in the case of WT strain upon addition of Congo red is shown to be consistent with an upregulation of Hsp12p in the close vicinity of the plasma membrane.

Yaws

MedlinePlus

... in 5 countries (Congo, Ghana, Papua New Guinea, Solomon Islands and Vanuatu) has provided promising results and ... in 5 countries (Congo, Ghana, Papua New Guinea, Solomon Islands and Vanuatu) has provided promising results and ...

Buruli Ulcer (Mycobacterium ulcerans Infection)

MedlinePlus

... Pacific WHO in countries » Overview Statistics Cooperation strategies Democratic Republic of the Congo » Emergencies Focus on » Bangladesh Rohingya Democratic Republic of the Congo Iraq Nigeria Somalia South ...

Global Health Observatory (GHO): Life Expectancy

MedlinePlus

... Overview Statistics Cooperation strategies Democratic Republic of the Congo » Emergencies Focus on » Bangladesh Rohingya Democratic Republic of the Congo Iraq Nigeria Somalia South Sudan Syrian Arab Republic ...

Palaeogeographic evolution of the central segment of the South Atlantic during Early Cretaceous times: palaeotopographic and geodynamic implications

NASA Astrophysics Data System (ADS)

Chaboureau, A. C.; Guillocheau, F.; Robin, C.; Rohais, S.; Moulin, M.; Aslanian, D.

2012-04-01

The tectonic and sedimentary evolution of the Early Cretaceous rift of the central segment of the South Atlantic Ocean is debated. Our objective is to better constraint the timing of its evolution by drawing palaeogeographic and deformation maps. Eight palaeogeographic and deformations maps were drawn from the Berriasian to the Middle-Late Aptian, based on a biostratigraphic (ostracodes and pollens) chart recalibrated on absolute ages (chemostratigraphy, interstratified volcanics, Re-Os dating of the organic matter). The central segment of the South Atlantic is composed of two domains that have a different history in terms of deformation and palaeogeography. The southern domain includes Namibe, Santos and Campos Basins. The northern domain extends from Espirito Santo and North Kwanza Basins, in the South, to Sergipe-Alagoas and North Gabon Basins to the North. Extension started in the northern domain during Late Berriasian (Congo-Camamu Basin to Sergipe-Alagoas-North Gabon Basins) and migrated southward. At that time, the southern domain was not a subsiding domain. This is time of emplacement of the Parana-Etendeka Trapp (Late Hauterivian-Early Barremian). Extension started in this southern domain during Early Barremian. The brittle extensional period is shorter in the South (5-6 Ma, Barremian to base Aptian) than in the North (19 to 20 Myr, Upper Berriasian to Base Aptian). From Late Berriasian to base Aptian, the northern domain evolves from a deep lake with lateral highs to a shallower one, organic-rich with no more highs. The lake migrates southward in two steps, until Valanginian at the border between the northern and southern domains, until Early Barremian, North of Walvis Ridge. The Sag phase is of Middle to Late Aptian age. In the southern domain, the transition between the brittle rift and the sag phase is continuous. In the northern domain, this transition corresponds to a hiatus of Early to Middle Aptian age, possible period of mantle exhumation. Marine influences were clearly occurring since the Early Aptian in the Northern domain and the Campos Basin. They seem sharp, brief flooding coming from the North, i.e. from the Tethys-Central Atlantic, trough a seaway crossing South America from Sao Luis, Parnaiba, Araripe and Almada basins (Arai, 1989). In the absence of data, the importance of those marine flooding during the Middle Aptian in the Santos Basin is still discussed. Keywords: South Atlantic Ocean, Early Cretaceous, Rift, Palaeogeography, Geodynamic

Frequently Asked Questions and Answers on Smallpox

MedlinePlus

... Overview Statistics Cooperation strategies Democratic Republic of the Congo » Emergencies Focus on » Bangladesh Rohingya Democratic Republic of the Congo Iraq Nigeria Somalia South Sudan Syrian Arab Republic ...

Rape as a weapon of war in the Democratic Republic of the Congo.

PubMed

Brown, Carly

2012-01-01

The Democratic Republic of the Congo has been appropriately acknowledged as "the rape capital of the world." While the country has been trapped in conflict, the use of rape as a weapon of war has been rampant and unyielding. The sexual violence inflicted upon women has been nothing less than brutal and destructive, physically, socially, and psychologically. This paper analyzes the use of rape as a weapon of war in the Congo, taking into context the ongoing war, cultural and social situations that facilitate its existence, and the many consequences the victims are forced to endure. Drawing information from various academic journals, articles, and field research from international organizations, this paper paints a concise picture of the sexual atrocities occurring in the Democratic Republic of the Congo.

Smallpox Inhibitor of Complement Enzymes (SPICE): Dissecting Functional Sites and Abrogating Activity1

PubMed Central

Liszewski, M. Kathryn; Leung, Marilyn K.; Hauhart, Richard; Fang, Celia J.; Bertram, Paula; Atkinson, John P.

2010-01-01

Although smallpox was eradicated as a global illness more than 30 years ago, variola virus and other related pathogenic poxviruses, such as monkeypox, remain potential bioterrorist weapons or could re-emerge as natural infections. Poxviruses express virulence factors that down-modulate the host’s immune system. We previously compared functional profiles of the poxviral complement inhibitors of smallpox, vaccinia, and monkeypox known as SPICE, VCP (or VICE), and MOPICE, respectively. SPICE was the most potent regulator of human complement and attached to cells via glycosaminoglycans. The major goals of the present study were to further characterize the complement regulatory and heparin binding sites of SPICE and to evaluate a mAb that abrogates its function. Using substitution mutagenesis, we established that (1) elimination of the three heparin binding sites severely decreases but does not eliminate glycosaminoglycan binding, (2) there is a hierarchy of activity for heparin binding among the three sites, and (3) complement regulatory sites overlap with each of the three heparin binding motifs. By creating chimeras with interchanges of SPICE and VCP residues, a combination of two SPICE amino acids (H77 plus K120) enhances VCP activity ~200-fold. Also, SPICE residue L131 is critical for both complement regulatory function and accounts for the electrophoretic differences between SPICE and VCP. An evolutionary history for these structure-function adaptations of SPICE is proposed. Finally, we identified and characterized a mAb that inhibits the complement regulatory activity of SPICE, MOPICE, and VCP and thus could be used as a therapeutic agent. PMID:19667083

15 CFR 2013.1 - Designations.

Code of Federal Regulations, 2012 CFR

2012-01-01

.... Afr. Rep. Chad Congo Côte d'Ivoire Dem. Rep. of the Congo Djibouti Egypt Gambia Ghana Guinea Guinea... Papua New Guinea Paraguay Peru Philippines South Africa St. Kitts & Nevis St. Lucia St. Vincent...

15 CFR 2013.1 - Designations.

Code of Federal Regulations, 2013 CFR

2013-01-01

.... Afr. Rep. Chad Congo Côte d'Ivoire Dem. Rep. of the Congo Djibouti Egypt Gambia Ghana Guinea Guinea... Papua New Guinea Paraguay Peru Philippines South Africa St. Kitts & Nevis St. Lucia St. Vincent...

15 CFR 2013.1 - Designations.

Code of Federal Regulations, 2014 CFR

2014-01-01

.... Afr. Rep. Chad Congo Côte d'Ivoire Dem. Rep. of the Congo Djibouti Egypt Gambia Ghana Guinea Guinea... Papua New Guinea Paraguay Peru Philippines South Africa St. Kitts & Nevis St. Lucia St. Vincent...

The wide-angle seismic image of a complex rifted margin, offshore North Namibia: Implications for the tectonics of continental breakup

NASA Astrophysics Data System (ADS)

Planert, Lars; Behrmann, Jan; Jokat, Wilfried; Fromm, Tanja; Ryberg, Trond; Weber, Michael; Haberland, Christian

2017-10-01

Voluminous magmatism during the South Atlantic opening has been considered as a classical example for plume related continental breakup. We present a study of the crustal structure around Walvis Ridge, near the intersection with the African margin. Two wide-angle seismic profiles were acquired. One is oriented NNW-SSE, following the continent-ocean transition and crossing Walvis Ridge. A second amphibious profile runs NW-SE from the Angola Basin into continental Namibia. At the continent-ocean boundary (COB) the mafic crust beneath Walvis Ridge is up to 33 km thick, with a pronounced high-velocity lower crustal body. Towards the south there is a smooth transition to 20-25 km thick crust underlying the COB in the Walvis Basin, with a similar velocity structure, indicating a gabbroic lower crust with associated cumulates at the base. The northern boundary of Walvis Ridge towards the Angola Basin shows a sudden change to oceanic crust only 4-6 km thick, coincident with the projection of the Florianopolis Fracture Zone, one of the most prominent tectonic features of the South Atlantic ocean basin. In the amphibious profile the COB is defined by a sharp transition from oceanic to rifted continental crust, with a magmatic overprint landward of the intersection of Walvis Ridge with the Namibian margin. The continental crust beneath the Congo Craton is 40 km thick, shoaling to 35 km further SE. The velocity models show that massive high-velocity gabbroic intrusives are restricted to a narrow zone directly underneath Walvis Ridge and the COB in the south. This distribution of rift-related magmatism is not easily reconciled with models of continental breakup following the establishment of a large, axially symmetric plume in the Earth's mantle. Rift-related lithospheric stretching and associated transform faulting play an overriding role in locating magmatism, dividing the margin in a magma-dominated southern and an essentially amagmatic northern segment.

How are the wetlands over tropical basins impacted by the extreme hydrological events?

NASA Astrophysics Data System (ADS)

Al-Bitar, A.; Parrens, M.; Frappart, F.; Papa, F.; Kerr, Y. H.; Cretaux, J. F.; Wigneron, J. P.

2016-12-01

Wetlands play a crucial role in tropical basins and still many questions remain unanswered on how extreme events (like El-Nino) impacts them. Answering these questions is challenging as monitoring of inland water surfaces via remote sensing over tropical areas is a difficult task because of impact of vegetation and cloud cover. Several microwave based products have been elaborated to monitor these surfaces (Papa et al. 2010). In this study we combine the use of L-band microwave brightness temperatures and altimetric data from SARAL/ALTIKA to derive water storage maps at relatively high (7days) temporal frequency. The area of interest concerns the Amazon, Congo and GBH basins A first order radiative model is used to derive surface water over land from the brightness temperature measured by ESA SMOS mission at coarse resolution (25 km x 25 km) and 7-days frequency. An initial investigation of the use of the SMAP mission for the same purpose will be also presented. The product is compared to the static land cover map such as ESA CCI and the International Geosphere-Biosphere Program (IGBP) and also dynamic maps from SWAPS. It is then combined to the altimetric data to derive water storage maps. The water surfaces and water storage products are then compared to precipitation data from GPM TRMM datasets, ground water storage change from GRACE and river discharge data from field data. The amplitudes and time shifts of the signals is compared based on the sub-basin definition from Hydroshed database. The dataset is then divided into years of strong and weak El-Nino signal and the anomaly is between the two dataset is compared. The results show a strong influence of EL-Nino on the time shift of the different components showing that the hydrological regime of wetlands is highly impacted by these extreme events. This can have dramatic impacts on the ecosystem as the wetlands are vulnerable with a high biodiversity.

Crimean-Congo Hemorrhagic Fever (CCHF)

MedlinePlus

... Congo Hemorrhagic Fever (CCHF) [PDF – 2 pages] Virus Ecology Viral Hemorrhagic Fever (VHF) Information for Specific Groups ... Diagnosis Treatment Prevention Outbreak Distribution Map Resources Virus Ecology File Formats Help: How do I view different ...

Treatment of industrial wastewater containing Congo Red and Naphthol Green B using low-cost adsorbent.

PubMed

Attallah, M F; Ahmed, I M; Hamed, Mostafa M

2013-02-01

The present work investigates the potential use of metal hydroxides sludge (MHS) generated from hot dipping galvanizing plant for adsorption of Congo Red and Naphthol Green B dyes from aqueous solutions. Characterization of MHS included infrared and X-ray fluorescence analysis. The effect of shaking time, initial dye concentration, temperature, adsorbent dosage and pH has been investigated. The results of adsorption experiments indicate that the maximum capacity of Congo Red and Naphthol Green B dyes at equilibrium (q(e)) and percentage of removal at pH 6 are 40 mg/g, 93 %, and 10 mg/g, 52 %, respectively. Some kinetic models were used to illustrate the adsorption process of Congo Red and Naphthol Green B dyes using MHS waste. Thermodynamic parameters such as (ΔG, ΔS, and ΔH) were also determined.

Preparation and Characterization of Electro-Spun Fabricated Ag-TiO2 Composite Nanofibers and Its Enhanced Photo-Catalytic Activity for the Degradation of Congo Red.

PubMed

Jadhav, Arvind H; Zhang, Hongliang; Agyemang, Frank O; Hiremath, Vishwanath; Lee, Kyuyoung; Chandradass, Jeyaseelan; Seo, Jeong Gil; Kim, Hern

2015-10-01

Electro-spun fabricated TiO2 nanofibers were prepared by simple electro-spinning method, in subsequent step silver (Ag) was deposited using precipitation method and obtained Ag-TiO2 composite nanofibers. The properties and morphology of these prepared composite nanofibers were characterized by XRD, SEM, EDX, and TGA. The prepared electro-spun composite nanofibers were applied as catalyst for the photodegradation of Congo-red under immited solar light in aqueous solution. Result reveals that, Ag loaded TiO2 composite nanofibers were effectively increased photodegradation of Congo red compared with pure TiO2 nanofibers in analogous condition. As a result, 92.0% decomposition of Congo red was obtained by using 5 wt% of Ag loaded TiO2 composite nanofibers at room temperature in short reaction time using 300 W of solar light. In addition, photodegradation of Congo red was also studied under different experimental conditions such as amount of Ag loaded in TiO2 nanofibers and contact time. Moreover, we also studied sintering effect on TiO2 nanofibers and their consequent effect on photodegradation reaction. After completion of reaction, the nanofibers can be easily separated by filtration process and reused several times without significant loss of activity. Overall study reveals that, Ag-TiO2 composite nanofibers were strongly enhanced the surface activity for the photo catalytic degradation of Congo red under ambient condition.

Photocatalytic decomposition of Congo red under visible light irradiation using MgZnCr-TiO2 layered double hydroxide.

PubMed

Ma, Chi; Wang, Fenghua; Zhang, Chang; Yu, Zhigang; Wei, Jingjing; Yang, Zhongzhu; Li, Yongqiu; Li, Zihao; Zhu, Mengying; Shen, Liuqing; Zeng, Guangming

2017-02-01

The new nanophotocatalyst MgZnCr-TiO 2 was prepared by co-precipitation under different molar ratio of metals (Zn:Cr) and the loaded amount of TiO 2 . And it was characterized by X-ray diffraction, Raman spectroscopy, X-ray photoelectron spectroscopy et al. Langmuir model fitted well the adsorption isotherm with the value of R 2 0.9765, the maximum adsorption capacity was 526.32 mg g -1 , the adsorption followed pseudo second order kinetic by MgZnCr-TiO 2 (1:1:2-0.05). The photocatalytic oxidation of Congo red was used to determine the photocatalytic performance of MgZnCr-TiO 2 (1:1:2-0.05) under visible light irradiation, and the removal rate reached 98% after reaction for 40 min. The degradation mechanism of Congo red also was proposed, and the MgZnCr-TiO 2 (1:1:2-0.05) was stable after five cycles. Compared to the adsorption, Congo red was removed fundamentally by photocatalysis and it is expected to be an effective way to eliminate Congo red. Copyright © 2016 Elsevier Ltd. All rights reserved.

Soil-Atmosphere Greenhouse Gas Fluxes on the Margins of the Congo Forest: Effects of Forest Conversion towards Smallholder Agricultural Lands and Agricultural Intensification

NASA Astrophysics Data System (ADS)

kwatcho Kengdo, S.; Sonwa, D. J.; Njine-Bememba, C. B.; Djatsa, L. D.; Rufino, M. C.; Verchot, L. V.; Tejedor, J.; Dannenmann, M.

2016-12-01

The forests of the Congo Basin are subject to deforestation and land use change, which may severely influence the soil-atmosphere exchange of greenhouse gases (GHG). However, due to absence of analytical capacities in Central Africa, there is a lack of knowledge on fluxes of CO2, CH4 and N2O at the soil-atmosphere interface for natural and managed ecosystems, which introduces large uncertainties into regional and national GHG reporting. The objectives of this study were to quantify GHG emissions from typical land use on the margins of the Congo forests, to analyze seasonal variability and environmental controls of soil-atmosphere GHG fluxes across a land use gradient and explore options of sustainable intensification of maize cultivation. In Cameroon, we quantified fluxes of CO2, CH4, and N2O at the soil - atmosphere interface in secondary forests, cocoa agroforests, unfertilized mixed crop fields, and three different types of maize cultivation: unfertilized control, maize intercropped with N fixing beans, maize applied with mineral nitrogen fertilizer. We used manual static chamber techniques with approximately weekly temporal resolution over a full year and analyzed gas samples using a gas chromatograph. Soil temperature and moisture data were permanently recorded at main sites and soil sampling provided information on soil mineral N content. We found highest CO2 and N2O emissions, net CH4 uptake and soil mineral N concentrations in the secondary forest with lower values observed in cocoa agroforest and in particular in extensive mixed crop. Soil moisture changes were the dominant driver of seasonal changes of GHG fluxes at all study sites. Intercropping with N fixing beans did not alter soil N2O emissions from maize fields. In contrast, application of mineral N increased soil N2O emissions by more than a factor of five. Our work highlights the importance of soil moisture as the driver of GHG fluxes and in particular for N2O indicates a strong decrease in soil emissions after forest conversion to extensive crop fields. Agricultural intensification based on mineral N fertilizer can increase N2O emissions to levels significantly higher than those observed in secondary forests. Our trial with intercropped N fixing beans show promise towards a sustainable intensification of agriculture in the study region.

High-resolution vegetation dynamics reconstitution in the Zaire/Congo watershed since MIS 6

NASA Astrophysics Data System (ADS)

Dalibard, Mathieu; Popescu, Speranta-Maria; Maley, Jean; Pittet, Bernard; Marsset, Tania; Baudin, François; Dennielou, Bernard; Sionneau, Thomas; Escarguel, Gilles; Droz, Laurence

2010-05-01

The present-day latitudinal migrations of the Intertropical Convergence Zone (ITCZ) are controlled by ocean/atmosphere dynamics impact seasonality of monsoon influence on the intertropical eastern Atlantic and western Africa. The geographical position of the Zaire/Congo drainage basin spanning the Northern and Southern hemispheres makes it a key area to study variation of the climatic parameters (temperature and monsoon activity) through time. To identify the ITCZ variability during the last 180 ka, a multiproxy analysis (pollen grains, elemental ratio derived from XRF analysis, organic matter content, clay mineralogy) was performed on the core KZAI-02, drilled offshore Angola at 3418 m water depth. Pollen record indicates a very high plant diversity (327 taxa representative of 106 families). They have been grouped as follow with respect to their ecological requirements: (1) mangrove, (2) rain forest, (3) warm-temperate forest, (4) pioneer forest, (5) afromontane forest, (6) savannah, (7) marshes. The relative fluctuation of these ecological groups during the last 180 ka allows us to reconstruct the dynamics of vegetation and its response to global climate forcing. Generally the glacial periods are characterized by the development of the afromontane forest (mainly Podocarpus) on reliefs while in lower altitudes the savannah (Fabaceae Papilionoidae, Poaceae, Zygophyllum, etc.) spreads in response to the relative precipitation decrease. During interglacials our records indicate a progressive development of forest environments, the pioneer forest (Alchornea, Bridelia, Cnestis, etc.) being progressively replaced by the tropical rain forest (Acanthaceae, Fabaceae Caesalpinoideae, Sapotaceae, etc.). This evolution indicates an increase in temperature and humidity. At the stadial/interglacial transitions the development of the mangrove (Rhizophoraceae, Avicenia, Sonneratia, etc.) seems to respond principally to sea level rise. The maximum extension of Cyperaceae marshes contemporaneously with a significant presence of afromontane forest in MIS 6 may be correlated with rainfall increase probably related to changes in the monsoon activity. The information in term of humidity obtained from some other proxies (clay mineralogy, organic carbon, and elemental ratio measured by XRF, such as Br, etc.) shows a similar trend with the pollen record. A spectral analysis has been performed and reveals that the reconstructed climatic parameters from the Zaire/Congo watershed correlated with Milankovitch cycles, including semi-precession cycles (10 kyrs) characteristic of the Equatorial zone.

76 FR 63351 - Designation of Additional Individual Pursuant to Executive Order 13413

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-12

... Democratic Republic of Congo.'' DATES: The designation by the Director of OFAC of the individual identified... Code. In the Order, the President found that the situation in the Democratic Republic of the Congo...

Lamprologus markerti, a new lamprologine cichlid (Teleostei: Cichlidae) endemic to the lower Congo River in the Democratic Republic of Congo, west-central Africa.

PubMed

Tougas, Stephanie; Stiassny, Melanie L J

2014-08-15

A new Lamprologus is described from the lower Congo River (LCR) in the Democratic Republic of Congo. Lamprologus markerti, new species, is readily distinguished from L. tigripictilis and L. werneri, the LCR endemic lamprologines with which it was once taxonomically conflated, in the possession of a reduced number of gill rakers on the first arch (9-11 versus 12-17), a longer head (32.1-34.7% SL versus 29.3-31.9 and 29.1-32.9% SL, respectively), and a longer predorsal length (33.0-35.9% SL versus 29.3-32.7 and 28.5-32.6% SL, respectively). Further, L. markerti lacks a second intestinal loop present in both L. tigripictilis and L. werneri, and has a highly reduced infraorbital series often consisting of a single first infraorbital (lachrymal) element. 

Lithology, petrography and Cu occurrence of the Neoproterozoic glacial Mwale Formation at the Shanika syncline (Tenke Fungurume, Congo Copperbelt; Democratic Republic of Congo)

NASA Astrophysics Data System (ADS)

Mambwe, Pascal; Milan, Luke; Batumike, Jacques; Lavoie, Sébastien; Jébrak, Michel; Kipata, Louis; Chabu, Mumba; Mulongo, Sonya; Lubala, Toto; Delvaux, Damien; Muchez, Philippe

2017-05-01

The Mwale Formation that constitutes the base of the Nguba Group in the Neoproterozoic Katanga Supergroup has recently attracted renewed interest for copper mineral exploration. We present new field observations combined with detailed logging and petrography of MWAS0001 drill hole at Shanika syncline in the Tenke Fungurume Mining District. Our study has enabled us to subdivide the Mwale Formation into 7 distinct sequences. This succession is host to glaciogenic, glaciomarine, glaciofluvial and glaciolacustrine deposits. Glaciomarine beds are typically a deposit by debris flow in deep water marine environment, induced by basin wide tectonics and glaciation influence. Glaciofluvial beds were deposited in shallow water, fluvial deltaic environment. The glaciolacustrine environment is indicated by dropstones occurring in the laminated mudstone and rhythmites with dispersed clasts observed in the siltstone and conglomerate. These beds are interlayered within the glaciogenic beds, and are characterised by variable clast composition (felsic, mafic and metamorphic). The clasts are very poorly sorted, angular, rounded to moderately rounded, faceted or striated, and supported in a sandy argillaceous or mud matrix. Two main episodes of sulphide mineralisation are distinguished in the Mwale Formation. The diagenetic episode consists of disseminated euhedral and framboidal pyrites. The hydrothermal episode is associated with Mg-metasomatism and characterised by low grade copper mineralisation that occurs (i) in veins filled with carbonate-chlorite and carbonate-quartz-chlorite-Cu sulphides, such as chalcocite, chalcopyrite and bornite, and (ii) as disseminated sulphides within the host rock. This second episode is late to post-orogenic and can be correlated with late brittle tectonics within the Lufilian arc. The other alteration types include silicification and potassic alteration; however, these alterations are not associated with mineralisation.

Can the hemoglobin characteristics of vesicomyid clam species influence their distribution in deep-sea sulfide-rich sediments? A case study in the Angola Basin

NASA Astrophysics Data System (ADS)

Decker, C.; Zorn, N.; Le Bruchec, J.; Caprais, J. C.; Potier, N.; Leize-Wagner, E.; Lallier, F. H.; Olu, K.; Andersen, A. C.

2017-08-01

Vesicomyids live in endosymbiosis with sulfur-oxidizing bacteria and therefore need hydrogen sulfide to survive. They can nevertheless live in a wide range of sulfide and oxygen levels and depths, which may explain the exceptional diversity of this clam family in deep-sea habitats. In the Gulf of Guinea, nine species of vesicomyid clams are known to live in cold-seep areas with pockmarks from 600 to 3200 m deep, as well as in the organic-rich sediments of the Congo deep-sea fan at 5000 m deep. Our previous study showed that two species living in a giant pockmark have different oxygen carriers, suggesting different adaptations to hypoxia. Here, we studied the hemoglobin structure and oxygen affinity in three other species, Calyptogena valdiviae, Elenaconcha guiness and Abyssogena southwardae to determine whether the characteristics of their oxygen carriers contribute to their distribution in sulfide-rich sediments at a regional scale. Documenting pairwise species associations in various proportions, we give a semi-quantitative account of their local distribution and oxygen and sulfide measurements at seven sites. Mass spectrometry showed that each vesicomyid species has four intracellular monomeric hemoglobin molecules of 15-16 kDa, all differing in their molecular mass. As expected, the monomers showed no cooperativity in oxygen binding. Their oxygen affinities were very high (below 1 Torr), but differed significantly. C. valdiviae had the highest affinity and was dominant in the Harp pockmark, the site with the lowest oxygen content (half the value of fully oxygenated water). A. southwardae dominated in the Congo Lobe area, the site with the deepest sulfides. We discuss how hemoglobin may favor an active, vertical distribution of vesicomyids in sulfide-rich sediments.

Density variations and their influence on carbon stocks: case-study on two Biosphere Reserves in the Democratic Republic of Congo

NASA Astrophysics Data System (ADS)

De Ridder, Maaike; De Haulleville, Thalès; Kearsley, Elizabeth; Van den Bulcke, Jan; Van Acker, Joris; Beeckman, Hans

2014-05-01

It is commonly acknowledged that allometric equations for aboveground biomass and carbon stock estimates are improved significantly if density is included as a variable. However, not much attention is given to this variable in terms of exact, measured values and density profiles from pith to bark. Most published case-studies obtain density values from literature sources or databases, this way using large ranges of density values and possible causing significant errors in carbon stock estimates. The use of one single fixed value for density is also not recommended if carbon stock increments are estimated. Therefore, our objective is to measure and analyze a large number of tree species occurring in two Biosphere Reserves (Luki and Yangambi). Nevertheless, the diversity of tree species in these tropical forests is too high to perform this kind of detailed analysis on all tree species (> 200/ha). Therefore, we focus on the most frequently encountered tree species with high abundance (trees/ha) and dominance (basal area/ha) for this study. Increment cores were scanned with a helical X-ray protocol to obtain density profiles from pith to bark. This way, we aim at dividing the tree species with a distinct type of density profile into separate groups. If, e.g., slopes in density values from pith to bark remain stable over larger samples of one tree species, this slope could also be used to correct for errors in carbon (increment) estimates, caused by density values from simplified density measurements or density values from literature. In summary, this is most likely the first study in the Congo Basin that focuses on density patterns in order to check their influence on carbon stocks and differences in carbon stocking based on species composition (density profiles ~ temperament of tree species).

Human Exposure to Wild Animals in the Sankuru Province of the Democratic Republic of the Congo.

PubMed

Rimoin, Anne W; Alfonso, Vivian Helena; Hoff, Nicole A; Doshi, Reena H; Mulembakani, Prime; Kisalu, Nevile K; Muyembe, Jean-Jacques; Okitolonda, Emile W; Wright, Linda L

2017-09-01

Due to the high level of biological diversity in the Congo Basin and human population dependence on bushmeat, the DRC represents an ideal location for expanding knowledge on wild animal exposures and thus the potential for transmission of zoonotic pathogens. However, limited information exists on patterns and extent of contact with wildlife in such communities. Using a cross-sectional study, 14 villages in the Sankuru Province of the DRC were surveyed between August and September 2007. Villagers ≥ 1 year of age and at home of the time of the survey were eligible and enrolled to describe and assess factors associated with animal exposures (both activity and type of animal). Among respondents, 91% reported exposure to rodents, 89% to duikers, 78% to non-human primates (NHPs), and 32% reported contact with bats in the month prior to the survey. The most frequently reported activities included eating (95%), cooking (70%), and butchering or skinning of animals (55%). The activities and animals to which subjects had contact varied by sex and age. Moreover, we observed a high correlation of the same activities across animal types. In this and other populations that rely on bushmeat, there is a high frequency of exposure to multiple animal species through various modalities. In the event of future zoonotic disease outbreaks, effective public health interventions and campaigns that mitigate the risk of animal contact during outbreaks need to be broad to include various modes of contact and should be directed to both men and women across all age groups. As available information is limited, further studies are necessary to better understand the complex relationships and exposures individuals have with animals.

Geological Substrates Shape Tree Species and Trait Distributions in African Moist Forests

PubMed Central

Fayolle, Adeline; Engelbrecht, Bettina; Freycon, Vincent; Mortier, Frédéric; Swaine, Michael; Réjou-Méchain, Maxime; Doucet, Jean-Louis; Fauvet, Nicolas; Cornu, Guillaume; Gourlet-Fleury, Sylvie

2012-01-01

Background Understanding the factors that shape the distribution of tropical tree species at large scales is a central issue in ecology, conservation and forest management. The aims of this study were to (i) assess the importance of environmental factors relative to historical factors for tree species distributions in the semi-evergreen forests of the northern Congo basin; and to (ii) identify potential mechanisms explaining distribution patterns through a trait-based approach. Methodology/Principal Findings We analyzed the distribution patterns of 31 common tree species in an area of more than 700,000 km2 spanning the borders of Cameroon, the Central African Republic, and the Republic of Congo using forest inventory data from 56,445 0.5-ha plots. Spatial variation of environmental (climate, topography and geology) and historical factors (human disturbance) were quantified from maps and satellite records. Four key functional traits (leaf phenology, shade tolerance, wood density, and maximum growth rate) were extracted from the literature. The geological substrate was of major importance for the distribution of the focal species, while climate and past human disturbances had a significant but lesser impact. Species distribution patterns were significantly related to functional traits. Species associated with sandy soils typical of sandstone and alluvium were characterized by slow growth rates, shade tolerance, evergreen leaves, and high wood density, traits allowing persistence on resource-poor soils. In contrast, fast-growing pioneer species rarely occurred on sandy soils, except for Lophira alata. Conclusions/Significance The results indicate strong environmental filtering due to differential soil resource availability across geological substrates. Additionally, long-term human disturbances in resource-rich areas may have accentuated the observed patterns of species and trait distributions. Trait differences across geological substrates imply pronounced differences in population and ecosystem processes, and call for different conservation and management strategies. PMID:22905127

Functional community structure of African monodominant Gilbertiodendron dewevrei forest influenced by local environmental filtering.

PubMed

Kearsley, Elizabeth; Verbeeck, Hans; Hufkens, Koen; Van de Perre, Frederik; Doetterl, Sebastian; Baert, Geert; Beeckman, Hans; Boeckx, Pascal; Huygens, Dries

2017-01-01

Monodominant patches of forest dominated by Gilbertiodendron dewevrei are commonly found in central African tropical forests, alongside forests with high species diversity. Although these forests are generally found sparsely distributed along rivers, their occurrence is not thought to be (clearly) driven by edaphic conditions but rather by trait combinations of G. dewevrei that aid in achieving monodominance. Functional community structure between these monodominant and mixed forests has, however, not yet been compared. Additionally, little is known about nondominant species in the monodominant forest community. These two topics are addressed in this study. We investigate the functional community structure of 10 one-hectare plots of monodominant and mixed forests in a central region of the Congo basin, in DR Congo. Thirteen leaf and wood traits are measured, covering 95% (basal area weighted) of all species present in the plots, including leaf nutrient contents, leaf isotopic compositions, specific leaf area, wood density, and vessel anatomy. The trait-based assessment of G. dewevrei shows an ensemble of traits related to water use and transport that could be favorable for its location near forest rivers. Moreover, indications have been found for N and P limitations in the monodominant forest, possibly related to ectomycorrhizal associations formed with G. dewevrei . Reduced leaf N and P contents are found at the community level for the monodominant forest and for different nondominant groups, as compared to those in the mixed forest. In summary, this work shows that environmental filtering does prevail in the monodominant G. dewevrei forest, leading to lower functional diversity in this forest type, with the dominant species showing beneficial traits related to its common riverine locations and with reduced soil N and P availability found in this environment, both coregulating the tree community assembly.

Widespread decline of Congo rainforest greenness in the past decade.

PubMed

Zhou, Liming; Tian, Yuhong; Myneni, Ranga B; Ciais, Philippe; Saatchi, Sassan; Liu, Yi Y; Piao, Shilong; Chen, Haishan; Vermote, Eric F; Song, Conghe; Hwang, Taehee

2014-05-01

Tropical forests are global epicentres of biodiversity and important modulators of climate change, and are mainly constrained by rainfall patterns. The severe short-term droughts that occurred recently in Amazonia have drawn attention to the vulnerability of tropical forests to climatic disturbances. The central African rainforests, the second-largest on Earth, have experienced a long-term drying trend whose impacts on vegetation dynamics remain mostly unknown because in situ observations are very limited. The Congolese forest, with its drier conditions and higher percentage of semi-evergreen trees, may be more tolerant to short-term rainfall reduction than are wetter tropical forests, but for a long-term drought there may be critical thresholds of water availability below which higher-biomass, closed-canopy forests transition to more open, lower-biomass forests. Here we present observational evidence for a widespread decline in forest greenness over the past decade based on analyses of satellite data (optical, thermal, microwave and gravity) from several independent sensors over the Congo basin. This decline in vegetation greenness, particularly in the northern Congolese forest, is generally consistent with decreases in rainfall, terrestrial water storage, water content in aboveground woody and leaf biomass, and the canopy backscatter anomaly caused by changes in structure and moisture in upper forest layers. It is also consistent with increases in photosynthetically active radiation and land surface temperature. These multiple lines of evidence indicate that this large-scale vegetation browning, or loss of photosynthetic capacity, may be partially attributable to the long-term drying trend. Our results suggest that a continued gradual decline of photosynthetic capacity and moisture content driven by the persistent drying trend could alter the composition and structure of the Congolese forest to favour the spread of drought-tolerant species.

Phylodynamics of the HIV-1 CRF02_AG clade in Cameroon

PubMed Central

Faria, Nuno Rodrigues; Suchard, Marc A; Abecasis, Ana; Sousa, J. D.; Ndembi, Nicaise; Camacho, R.J.; Vandamme, Anne-Mieke; Peeters, Martine; Lemey, Philippe

2015-01-01

Evolutionary analyses have revealed an origin of pandemic HIV-1 group M in the Congo River basin in the first part of the XXth century, but the patterns of historical viral spread in or around its epicentre remain largely unexplored. Here, we combine epidemiologic and molecular sequence data to investigate the spatiotemporal patterns of the CRF02_AG clade. By explicitly integrating prevalence counts and genetic population size estimates we date the epidemic emergence of CRF02_AG at 1973.1 (1972.1, 1975.3 95% CI). To infer their phylogeographic signature at a regional scale, we analyze pol and env time-stamped sequence data from 8 countries using a Bayesian phylogeographic approach based on a discrete asymmetric model. Our data confirms a spatial origin of this clade in the Democratic Republic of Congo (DRC) and suggests that viral dissemination to Cameroon occurred at an early stage of the evolutionary history of CRF02_AG. We find considerable support for epidemiological linkage between neighbour countries. Compilation of ethnographic data suggests that well-supported viral migration was related with chance exportation events rather than by sustained human migratory flows. Finally, using sequence data from 15 locations in Cameroon, we use relaxed random walk models to explore the spatiotemporal dynamics of CRF02_AG at a finer geographical detail. Phylogeographic dispersal in continuous space reveals that at least two distinct CRF02_AG lineages are circulating in overlapping regions that are evolving at different evolutionary and diffusion rates. Altogether, by combining molecular and epidemiological data, our results provide a time scale for CRF02_AG, place its spatial root within the putative root of group-M diversity and propose a scenario for the spatiotemporal patterns of a successful HIV-1 lineage both at a regional and country-scale. PMID:21565285

Biosorption potential of synthetic dyes by heat-inactivated and live Lentinus edodes CCB-42 immobilized in loofa sponges.

PubMed

Gimenez, Gabriela Gregolin; Ruiz, Suelen Pereira; Caetano, Wilker; Peralta, Rosane Marina; Matioli, Graciette

2014-12-01

Lentinus edodes CCB-42 was immobilized in loofa sponges and applied to the biosorption of the synthetic dyes congo red, bordeaux red and methyl violet. Live immobilized microorganisms achieved average decolorations of congo red, bordeaux red and methyl violet of 97.8, 99.7 and 90.6 %, respectively. The loofa sponge was the support and the coadjuvant promoting dye adsorption. The biosorption conditions were optimized for each dye, yielding 30 °C, pH 5.0 and a 12 h reaction time for congo red; 25 °C, pH 3.0 and 36 h for bordeaux red; and 25 °C, pH 8.0 and 24 h for methyl violet. Operational stability was evaluated over five consecutive cycles, with both bordeaux red and congo red exhibiting decolorations above 90 %, while the decoloration of methyl violet decreased after the third cycle. In the sixth month of storage, congo red, bordeaux red and methyl violet had decolorations of 93.1, 79.4 and 73.8 %, respectively. Biosorption process best fit the pseudo-second-order kinetic and Freundlich isotherm models. Maximum biosorption capacity of heat-treated L. edodes immobilized in loofa sponge was determined as 143.678, 500.00 and 381.679 mg/g for congo red, bordeaux red and methyl violet, respectively. Treatment with immobilized L. edodes reduced the phytotoxicity of the medium containing dyes. FT-Raman experiments suggested the occurrence of interactions between loofa sponge fibers, L. edodes and dye. L. edodes CCB-42 immobilized in loofa sponges represents a promising new mode of treatment of industrial effluents.

75 FR 61239 - Request for Public Comments on Annual Review of Country Eligibility for Benefits Under the...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-04

... Verde, Republic of Cameroon, Republic of Chad, Federal Islamic Republic of Comoros, Republic of Congo, Democratic Republic of Congo, Republic of Djibouti, Ethiopia, Gabonese Republic, The Gambia, Republic of...

The Possibility of Using the ICR Mouse as an Animal Model to Assess Antimonkeypox Drug Efficacy.

PubMed

Sergeev, Al A; Kabanov, A S; Bulychev, L E; Sergeev, Ar A; Pyankov, O V; Bodnev, S A; Galahova, D O; Zamedyanskaya, A S; Titova, K A; Glotov, A G; Taranov, O S; Omigov, V V; Shishkina, L N; Agafonov, A P; Sergeev, A N

2016-10-01

As a result of the conducted experimental studies on intranasal challenge of ICR mice, rabbits and miniature pigs (even in the maximum variant) with the doses of 4.0-5.5 lg PFU of monkeypox virus (MPXV), some clinical signs such as purulent conjunctivitis, blepharitis and ruffled fur were found only in mice. The 50% infective dose (C ID50 ) of MPXV for these animals estimated by the presence of external clinical signs was 4.8 lg PFU, and L ID50 estimated by the virus presence in the lungs of mice 7 days post-infection taking into account its 10% application in the animal respiratory tract was 1.4 lg PFU. When studying the dynamics of MPXV propagation in mice challenged intranasally with 25 L ID50 of MPXV, the maximum pathogen accumulation was revealed in nasal cavity, lungs and brain: 5.7 ± 0.1, 5.5 ± 0.1 and 5.3 ± 0.3 lg PFU/ml, respectively. The pathomorphological examination of these animals revealed the presence and replication of the pathogen in the traditional primary target cells for MPXV (mononuclear phagocyte system cells and respiratory tract epitheliocytes) as well as in some other types of cells (endothelial cells, reticular cells, connective tissue cells). Our use of these animals to assess the antiviral efficacy of some drugs demonstrated the agreement of the results (a significant positive effect of NIOCH-14 and ST-246) with those described in scientific literature, which opens up the prospects of using ICR mice as animal models for monkeypox to develop preventive antismallpox drugs. © 2015 Blackwell Verlag GmbH.

Zoonotic Poxviruses Associated with Companion Animals

PubMed Central

Tack, Danielle M.; Reynolds, Mary G.

2011-01-01

Simple Summary Contemporary enthusiasm for the ownership of exotic animals and hobby livestock has created an opportunity for the movement of poxviruses—such as monkeypox, cowpox, and orf—outside their traditional geographic range bringing them into contact with atypical animal hosts and groups of people not normally considered at risk. It is important that pet owners and practitioners of human and animal medicine develop a heightened awareness for poxvirus infections and understand the risks that can be associated with companion animals and livestock. This article reviews the epidemiology and clinical features of zoonotic poxviruses that are most likely to affect companion animals. Abstract Understanding the zoonotic risk posed by poxviruses in companion animals is important for protecting both human and animal health. The outbreak of monkeypox in the United States, as well as current reports of cowpox in Europe, point to the fact that companion animals are increasingly serving as sources of poxvirus transmission to people. In addition, the trend among hobbyists to keep livestock (such as goats) in urban and semi-urban areas has contributed to increased parapoxvirus exposures among people not traditionally considered at high risk. Despite the historic notoriety of poxviruses and the diseases they cause, poxvirus infections are often missed. Delays in diagnosing poxvirus-associated infections in companion animals can lead to inadvertent human exposures. Delays in confirming human infections can result in inappropriate treatment or prolonged recovery. Early recognition of poxvirus-associated infections and application of appropriate preventive measures can reduce the spread of virus between companion animals and their owners. This review will discuss the epidemiology and clinical features associated with the zoonotic poxvirus infections most commonly associated with companion animals. PMID:26486622

Poxvirus Antigen Staining of Immune Cells as a Biomarker to Predict Disease Outcome in Monkeypox and Cowpox Virus Infection in Non-Human Primates

PubMed Central

Song, Haifeng; Janosko, Krisztina; Johnson, Reed F.; Qin, Jing; Josleyn, Nicole; Jett, Catherine; Byrum, Russell; Claire, Marisa St.; Dyall, Julie; Blaney, Joseph E.; Jennings, Gerald; Jahrling, Peter B.

2013-01-01

Infection of non-human primates (NHPs) such as rhesus and cynomolgus macaques with monkeypox virus (MPXV) or cowpox virus (CPXV) serve as models to study poxvirus pathogenesis and to evaluate vaccines and anti-orthopox therapeutics. Intravenous inoculation of macaques with high dose of MPXV (>1–2×107 PFU) or CPXV (>102 PFU) results in 80% to 100% mortality and 66 to 100% mortality respectively. Here we report that NHPs with positive detection of poxvirus antigens in immune cells by flow cytometric staining, especially in monocytes and granulocytes succumbed to virus infection and that early positive pox staining is a strong predictor for lethality. Samples from four independent studies were analyzed. Eighteen NHPs from three different experiments were inoculated with two different MPXV strains at lethal doses. Ten NHPs displayed positive pox-staining and all 10 NHPs reached moribund endpoint. In contrast, none of the three NHPs that survived anticipated lethal virus dose showed apparent virus staining in the monocytes and granulocytes. In addition, three NHPs that were challenged with a lethal dose of MPXV and received cidofovir treatment were pox-antigen negative and all three NHPs survived. Furthermore, data from a CPXV study also demonstrated that 6/9 NHPs were pox-antigen staining positive and all 6 NHPs reached euthanasia endpoint, while the three survivors were pox-antigen staining negative. Thus, we conclude that monitoring pox-antigen staining in immune cells can be used as a biomarker to predict the prognosis of virus infection. Future studies should focus on the mechanisms and implications of the pox-infection of immune cells and the correlation between pox-antigen detection in immune cells and disease progression in human poxviral infection. PMID:23577120

Velocity mapping in the Lower Congo River: a first look at the unique bathymetry and hydrodynamics of Bulu Reach

USGS Publications Warehouse

Jackson, P. Ryan; Oberg, Kevin A.; Gardiner, Ned; Shelton, John

2009-01-01

The lower Congo River is one of the deepest, most powerful, and most biologically diverse stretches of river on Earth. The river’s 270 m decent from Malebo Pool though the gorges of the Crystal Mountains to the Atlantic Ocean (498 km downstream) is riddled with rapids, cataracts, and deep pools. Much of the lower Congo is a mystery from a hydraulics perspective. However, this stretch of the river is a hotbed for biologists who are documenting evolution in action within the diverse, but isolated, fish populations. Biologists theorize that isolation of fish populations within the lower Congo is due to barriers presented by flow structure and bathymetry. To investigate this theory, scientists from the U.S. Geological Survey and American Museum of Natural History teamed up with an expedition crew from National Geographic in 2008 to map flow velocity and bathymetry within target reaches in the lower Congo River using acoustic Doppler current profilers (ADCPs) and echo sounders. Simultaneous biological and water quality sampling was also completed. This paper presents some preliminary results from this expedition, specifically with regard to the velocity structure andbathymetry. Results show that the flow in the bedrock controlled Bulu reach of the lower Congo is highly energetic. Turbulent and secondary flow structures can span the full depth of flow (up to 165 m), while coherent bank-to-bank cross-channel flow structures are absent. Regions of flow separation near the banks are isolated from one another and from the opposite bank by high shear, high velocity zones with depth-averaged flow velocities that can exceed 4 m/s.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jones, T.P.

Sensors for the determination of pH have been developed which are based on the immobilization of direct dyes at hydrolyzed cellulosic films. The performance and structural characteristics of the sensors were investigated by a variety of spectroscopic methods, and applications for remote sensing were developed. Films of cellulose acetate were base hydrolyzed in 0.07 M KOH to yield a porous support structure. The structural changes resulting from the hydrolysis on cellulose acetate were probed with infrared internal reflectance spectroscopy. The progress of the hydrolysis reaction was monitored by the changes in vibrational modes of the acetyl group, and other spectralmore » changes indicated changes in film thickness as a result of solvent incorporation. Direct dyes, including Congo Red and C. I. Direct Blue 8, were then immobilized at these porous cellulosic films. The optical response characteristics of the Congo Red pH sensor were characterized, including the UV-visible absorption spectra as a function of pH, the response time as a function of ionic strength and ionic size of electrolyte, the long-term stability of the sensor, the effects of metal-ion interference, and the concentration of Congo Red in the polymer film. The structural characteristics of the sensor were investigated by internal reflectance spectroscopy and resonance-enhanced Raman spectroscopy, and the protonation sites were identified as the two azo groups of Congo Red. Infrared internal reflection spectra of immobilized Congo Red led to the development of a sensor for pH based on infrared spectroscopy. Finally, a two-wavelength fiber-optic photometer, which is based on solid-state sources and detectors, and a fiber-optic photometer, which is based on solid-state sources and detectors, and a fiber-optic probe were developed for pH determinations using Congo Red and C. I. Direct Blue 8 pH sensors.« less

Synthesis of Magnesium Ferrites for the Adsorption of Congo Red from Aqueous Solution Using Batch Studies

NASA Astrophysics Data System (ADS)

Erdawati, E.; Darsef, D.

2018-04-01

A sol gel method with citric acid as an anionic surfactant was used to fabricate nano magnesium ferrites (MgFe2O4) under different calcination temperatures for 2h, respectively. The microstructure and surface morphology of magnesium ferrite powder were characterized by FTIR, XRD, SEM, and BET. The results of this study are useful for adsorption Congo red. The results showed that increasing solution pH and extending contact time are favorable for improving adsorption efficiency. with initial Congo red concentration of 50 mg/L and 100 mg/L. Adsorption data fits well with the Langmuir isotherm models with a maximum adsorption capacity (qm) and a Langmuir adsorption equilibrium constant (K) of 65.1 mg/g and 0.090 L/mg, respectively. The adsorption kinetic agrees well with pseudo second order model with the pseudo second rate constants (K2) of 0.0468 and0.00189 g/mg/min for solutions with initial congo red of 50 and 100 mg/L, respectively

Jute stick powder as a potential biomass for the removal of congo red and rhodamine B from their aqueous solution.

PubMed

Panda, Gopal C; Das, Sujoy K; Guha, Arun K

2009-05-15

Jute stick powder (JSP) has been found to be a promising material for adsorptive removal of congo red (C.I. 22120) and rhodamine B (C.I. 45170) from aqueous solutions. Physico-chemical parameters like dye concentration, solution pH, temperature and contact time have been varied to study the adsorption phenomenon. Favorable adsorption occurs at around pH 7.0 whereas temperature has no significant effect on adsorption of both the dyes. The maximum adsorption capacity has been calculated to be 35.7 and 87.7mg/g of the biomass for congo red and rhodamine B, respectively. The adsorption process is in conformity with Freundlich and Langmuir isotherms for rhodamine B whereas congo red adsorption fits well to Langmuir isotherm only. In both the cases, adsorption occurs very fast initially and attains equilibrium within 60min. Kinetic results suggest the intra-particle diffusion of dyes as rate limiting step.

Serosurvey for Crimean-Congo hemorrhagic fever virus infections in ruminants in Katanga province, Democratic Republic of the Congo.

PubMed

Sas, Miriam A; Mertens, Marc; Kadiat, Jean G; Schuster, Isolde; Pongombo, Célestin P S; Maloba, Alois G K; Groschup, Martin H

2017-10-01

Crimean-Congo hemorrhagic fever virus (CCHFV) has been detected in many African countries. Unfortunately, little is known about the current CCHFV situation in most of those countries including the Democratic Republic of the Congo (DRC). In over 50 years, three human CCHF cases have been detected in DRC but no seroepidemiological investigation was performed so far. To determine the prevalence of CCHFV-specific antibodies we tested 838 serum samples of cattle, goat and sheep from the southern province Katanga, DRC. The detected seroprevalence in ruminants was 1.6% ranging from 0.4% to 3.4% between the two sampling sites, Kamina and Lubumbashi. The low prevalence indicates only sporadic introduction of CCHFV into this part of the country. DRC is a very large country and the study was performed only at two locations in one province; therefore, the investigations can be only a starting point for further epidemiological activities. Copyright © 2017 Elsevier GmbH. All rights reserved.

Identification of diverse Bartonella genotypes among small mammals from Democratic Republic of Congo and Tanzania.

PubMed

Gundi, Vijay A K B; Kosoy, Michael Y; Makundi, Rhodes H; Laudisoit, Anne

2012-08-01

Small mammals from the Democratic Republic (DR) of the Congo and Tanzania were tested to determine the prevalence and genetic diversity of Bartonella species. The presence of Bartonella DNA was assessed in spleen samples of the animals by rpoB- and gltA-polymerase chain reactions (PCRs). By rpoB-PCR, Bartonella was detected in 8 of 59 animals of DR Congo and in 16 of 39 Tanzanian animals. By gltA-PCR, Bartonella was detected in 5 and 15 animals of DR Congo and Tanzania, respectively. The gene sequences from Arvicanthis neumanni were closely related to Bartonella elizabethae. The genotypes from Lophuromys spp. and from Praomys delectorum were close to Bartonella tribocorum. Five genogroups were not genetically related to any known Bartonella species. These results suggest the need to conduct further studies to establish the zoonotic risks linked with those Bartonella species and, in particular, to verify whether these agents might be responsible for human cases of febrile illness of unknown etiology in Africa.

Concentrations and forms of heavy metals around two ore processing sites in Katanga, Democratic Republic of Congo

NASA Astrophysics Data System (ADS)

Mees, F.; Masalehdani, M. N. N.; De Putter, T.; D'Hollander, C.; Van Biezen, E.; Mujinya, B. B.; Potdevin, J. L.; Van Ranst, E.

2013-01-01

The concentration of heavy metals and the forms in which they occur were determined for tailings and derived deposits of two major processing sites of Cu-Co and Cu-Zn-(Pb) ores in the Katanga Copperbelt (Kipushi, Likasi). They were studied by a combination of methods, focussed on the nature of water- and EDTA-extractable compounds, the mineralogical composition of tailings and associated secondary minerals, and textural features of metal-bearing efflorescences. For the Kipushi area, sulfide minerals in tailings of decantation basins are identified as the source of extractable metals they contain, but input from an external source rather than local oxidation of substrate components is responsible for high levels of contamination in the Likasi area. Contaminated areas around Likasi are characterized by an abundance of Mg-sulfate efflorescences with high concentrations of cobalt and other metals, acting as an important vector for further dispersion of contaminants by wind and water.

15 CFR Supplement No. 2 to Part 745 - States Parties to the Convention on the Prohibition of the Development, Production, Stockpiling...

Code of Federal Regulations, 2010 CFR

2010-01-01

... Chile China* Colombia Comoros Congo (Democratic Republic of the) Congo (Republic of the) Cook Islands... Antilles. * For CWC purposes only, China includes Hong Kong and Macau. [64 FR 27143, May 18, 1999, as...

15 CFR Supplement No. 2 to Part 745 - States Parties to the Convention on the Prohibition of the Development, Production, Stockpiling...

Code of Federal Regulations, 2011 CFR

2011-01-01

... Chile China* Colombia Comoros Congo (Democratic Republic of the) Congo (Republic of the) Cook Islands... Antilles. * For CWC purposes only, China includes Hong Kong and Macau. [64 FR 27143, May 18, 1999, as...

The Ring Structure of Wembo-Nyama (Eastern Kasai, R.D. Congo): A Possible Impact Crater in Central Africa

NASA Astrophysics Data System (ADS)

Monegato, G.; Massironi, M.; Martellato, E.

2010-03-01

The structure of Wembo-Nyama (Eastern Kasai, R.D. Congo) is a new ring structure found in Central Africa. We discuss the possibility of an impact origin, considering its geomorphological characteristics and the geology of the area.

A new genus of Grapholitini from Africa related to Thaumatotibia (Lepidoptera: Tortricidae)

USDA-ARS?s Scientific Manuscript database

Thaumatovalva, new genus, is described and illustrated from the Afrotropical region. As currently defined the genus includes four species: T. deprinsorum, new species, from the Democratic Republic of Congo; T. albolineana, new species (type species), from the Democratic Republic of Congo; T. spinai ...

Explore various co-substrates for simultaneous electricity generation and Congo red degradation in air-cathode single-chamber microbial fuel cell.

PubMed

Cao, Yunqing; Hu, Yongyou; Sun, Jian; Hou, Bin

2010-08-01

Microbial fuel cell (MFC) holds a great promise to harvest electricity directly from a wide range of ready degradable organic matters and enhance degradation of some recalcitrant contaminants. Glucose, acetate sodium and ethanol were separately examined as co-substrates for simultaneous bioelectricity generation and Congo red degradation in a proton exchange membrane (PEM) air-cathode single-chamber MFC. The batch test results showed that more than 98% decolorization at the dye concentration of 300 mg/L were achieved within 36 h for all tested co-substrates during electricity generation. The decolorization rate was different with the co-substrates used. The fastest decolorization rate was achieved with glucose followed by ethanol and sodium acetate. Accumulated intermediates were observed during Congo red degradation which was demonstrated by UV-Visible spectra and high performance liquid chromatography (HPLC). Electricity generation was sustained and not significantly affected by the Congo red degradation. Glucose, acetate sodium and ethanol produced maximum power densities of 103 mW/m(2), 85.9 mW/m(2) and 63.2 mW/m(2), respectively, and the maximum voltage output decreased by only 7% to 15%. Our results demonstrated the feasibility of using various co-substrates for simultaneous decolorization of Congo red and bioelectricity generation in the MFC and showed that glucose was the preferred co-substrate. Copyright (c) 2009 Elsevier B.V. All rights reserved.

Establishing and validating the fluorescent amyloid ligand h-FTAA (heptamer formyl thiophene acetic acid) to identify transthyretin amyloid deposits in carpal tunnel syndrome.

PubMed

Hahn, Katharina; Nilsson, K Peter R; Hammarström, Per; Urban, Peter; Meliss, Rolf Rüdiger; Behrens, Hans-Michael; Krüger, Sandra; Röcken, Christoph

2017-06-01

Transthyretin-derived (ATTR) amyloidosis is a frequent finding in carpal tunnel syndrome. We tested the following hypotheses: the novel fluorescent amyloid ligand heptameric formic thiophene acetic acid (h-FTAA) has a superior sensitivity for the detection of amyloid compared with Congo red-staining; Amyloid load correlates with patient gender and/or patient age. We retrieved 208 resection specimens obtained from 184 patients with ATTR amyloid in the carpal tunnel. Serial sections were stained with Congo red, h-FTAA and an antibody directed against transthyretin (TTR). Stained sections were digitalized and forwarded to computational analyses. The amount of amyloid was correlated with patient demographics. Amyloid stained intensely with h-FTAA and an anti-TTR-antibody. Congo red-staining combined with fluorescence microscopy was significantly less sensitive than h-FTAA-fluorescence and TTR-immunostaining: the highest percentage area was found in TTR-immunostained sections, followed by h-FTAA and Congo red. The Pearson correlation coefficient was .8 (Congo red vs. h-FTAA) and .9 (TTR vs. h-FTAA). Amyloid load correlated with patient gender, anatomical site and patient age. h-FTAA is a highly sensitive method to detect even small amounts of ATTR amyloid in the carpal tunnel. The staining protocol is easy and h-FTAA may be a much more sensitive procedure to detect amyloid at an earlier stage.

In Vitro Characterization of a Nineteenth-Century Therapy for Smallpox

PubMed Central

Arndt, William; Mitnik, Chandra; Denzler, Karen L.; White, Stacy; Waters, Robert; Jacobs, Bertram L.; Rochon, Yvan; Olson, Victoria A.; Damon, Inger K.; Langland, Jeffrey O.

2012-01-01

In the nineteenth century, smallpox ravaged through the United States and Canada. At this time, a botanical preparation, derived from the carnivorous plant Sarracenia purpurea, was proclaimed as being a successful therapy for smallpox infections. The work described characterizes the antipoxvirus activity associated with this botanical extract against vaccinia virus, monkeypox virus and variola virus, the causative agent of smallpox. Our work demonstrates the in vitro characterization of Sarracenia purpurea as the first effective inhibitor of poxvirus replication at the level of early viral transcription. With the renewed threat of poxvirus-related infections, our results indicate Sarracenia purpurea may act as another defensive measure against Orthopoxvirus infections. PMID:22427855

In vitro characterization of a nineteenth-century therapy for smallpox.

PubMed

Arndt, William; Mitnik, Chandra; Denzler, Karen L; White, Stacy; Waters, Robert; Jacobs, Bertram L; Rochon, Yvan; Olson, Victoria A; Damon, Inger K; Langland, Jeffrey O

2012-01-01

In the nineteenth century, smallpox ravaged through the United States and Canada. At this time, a botanical preparation, derived from the carnivorous plant Sarracenia purpurea, was proclaimed as being a successful therapy for smallpox infections. The work described characterizes the antipoxvirus activity associated with this botanical extract against vaccinia virus, monkeypox virus and variola virus, the causative agent of smallpox. Our work demonstrates the in vitro characterization of Sarracenia purpurea as the first effective inhibitor of poxvirus replication at the level of early viral transcription. With the renewed threat of poxvirus-related infections, our results indicate Sarracenia purpurea may act as another defensive measure against Orthopoxvirus infections.

The Congolobe project, a multidisciplinary study of Congo deep-sea fan lobe complex: Overview of methods, strategies, observations and sampling

NASA Astrophysics Data System (ADS)

Rabouille, C.; Olu, K.; Baudin, F.; Khripounoff, A.; Dennielou, B.; Arnaud-Haond, S.; Babonneau, N.; Bayle, C.; Beckler, J.; Bessette, S.; Bombled, B.; Bourgeois, S.; Brandily, C.; Caprais, J. C.; Cathalot, C.; Charlier, K.; Corvaisier, R.; Croguennec, C.; Cruaud, P.; Decker, C.; Droz, L.; Gayet, N.; Godfroy, A.; Hourdez, S.; Le Bruchec, J.; Saout, J.; Le Saout, M.; Lesongeur, F.; Martinez, P.; Mejanelle, L.; Michalopoulos, P.; Mouchel, O.; Noel, P.; Pastor, L.; Picot, M.; Pignet, P.; Pozzato, L.; Pruski, A. M.; Rabiller, M.; Raimonet, M.; Ragueneau, O.; Reyss, J. L.; Rodier, P.; Ruesch, B.; Ruffine, L.; Savignac, F.; Senyarich, C.; Schnyder, J.; Sen, A.; Stetten, E.; Sun, Ming Yi; Taillefert, M.; Teixeira, S.; Tisnerat-Laborde, N.; Toffin, L.; Tourolle, J.; Toussaint, F.; Vétion, G.; Jouanneau, J. M.; Bez, M.; Congolobe Group:

2017-08-01

The presently active region of the Congo deep-sea fan (around 330,000 km2), called the terminal lobes or lobe complex, covers an area of 2500 km2 at 4700-5100 m water depth and 750-800 km offshore. It is a unique sedimentary area in the world ocean fed by a submarine canyon and a channel-levee system which presently deliver large amounts of organic carbon originating from the Congo River by turbidity currents. This particularity is due to the deep incision of the shelf by the Congo canyon, up to 30 km into the estuary, which funnels the Congo River sediments into the deep-sea. The connection between the river and the canyon is unique for major world rivers. In 2011, two cruises (WACS leg 2 and Congolobe) were conducted to simultaneously investigate the geology, organic and inorganic geochemistry, and micro- and macro-biology of the terminal lobes of the Congo deep-sea fan. Using this multidisciplinary approach, the morpho-sedimentary features of the lobes were characterized along with the origin and reactivity of organic matter, the recycling and burial of biogenic compounds, the diversity and function of bacterial and archaeal communities within the sediment, and the biodiversity and functioning of the faunal assemblages on the seafloor. Six different sites were selected for this study: Four distributed along the active channel from the lobe complex entrance to the outer rim of the sediment deposition zone, and two positioned cross-axis and at increasing distance from the active channel, thus providing a gradient in turbidite particle delivery and sediment age. This paper aims to provide the general context of this multidisciplinary study. It describes the general features of the site and the overall sampling strategy and provides the initial habitat observations to guide the other in-depth investigations presented in this special issue. Detailed bathymetry of each sampling site using 0.1-1 m resolution multibeam obtained with a remotely operated vehicle (ROV) shows progressive widening and smoothing of the channel-levees with increasing depth and reveals a complex morphology with channel bifurcations, erosional features and massive deposits. Dense ecosystems surveyed in the study area gather high density clusters of two large-sized species of symbiotic Vesicomyidae bivalves and microbial mats. These assemblages, which are rarely observed in sedimentary zones, resemble those based on chemosynthesis at cold-seep sites, such as the active pockmarks encountered along the Congo margin, and share with these sites the dominant vesicomyid species Christineconcha regab. Sedimentation rates estimated in the lobe complex range between 0.5 and 10 cm yr-1, which is 2-3 orders of magnitude higher than values generally encountered at abyssal depths. The bathymetry, faunal assemblages and sedimentation rates make the Congo lobe complex a highly peculiar deep-sea habitat driven by high inputs of terrigenous material delivered by the Congo channel-levee system.

Democratic Republic of Congo: Status of women in physics

NASA Astrophysics Data System (ADS)

Banza, Elvire Nzeba

2015-12-01

There is one physics department in the Democratic Republic of Congo, at the University of Kinshasa. Since 2001, one woman graduate continued her studies in England, where she received a PhD, and currently two master's degree graduates from this program have joined the faculty as assistant professors.

Gender, Poverty, Family Structure, and Investments in Children's Education in Kinshasa, Congo.

ERIC Educational Resources Information Center

Shapiro, David; Tambashe, B. Oleko

2001-01-01

Examines school enrollment and educational attainment in Kinshasa, Congo, focusing on how poverty, household structure, gender, and economic well-being affect investments in children's education. Increased economic well-being translates into greater attainment for both females and males, but does not necessarily reduce gender differences in school…

Exposure to Lyssaviruses in Bats of the Democratic Republic of the Congo.

PubMed

Kalemba, Lem's N; Niezgoda, Michael; Gilbert, Amy T; Doty, Jeffrey B; Wallace, Ryan M; Malekani, Jean M; Carroll, Darin S

2017-04-01

Lyssavirus infections in the Democratic Republic of Congo are poorly documented. We examined 218 bats. No lyssavirus antigens were detected but Lagos bat virus (LBV) neutralizing antibodies (VNA) were detected in Eidolon helvum and Myonycteris torquata . Four samples with LBV VNA reacted against Shimoni bat virus.

Migration of Chadic speaking pastoralists within Africa based on population structure of Chad Basin and phylogeography of mitochondrial L3f haplogroup.

PubMed

Cerný, Viktor; Fernandes, Verónica; Costa, Marta D; Hájek, Martin; Mulligan, Connie J; Pereira, Luísa

2009-03-23

Chad Basin, lying within the bidirectional corridor of African Sahel, is one of the most populated places in Sub-Saharan Africa today. The origin of its settlement appears connected with Holocene climatic ameliorations (aquatic resources) that started ~10,000 years before present (YBP). Although both Nilo-Saharan and Niger-Congo language families are encountered here, the most diversified group is the Chadic branch belonging to the Afro-Asiatic language phylum. In this article, we investigate the proposed ancient migration of Chadic pastoralists from Eastern Africa based on linguistic data and test for genetic traces of this migration in extant Chadic speaking populations. We performed whole mitochondrial genome sequencing of 16 L3f haplotypes, focused on clade L3f3 that occurs almost exclusively in Chadic speaking people living in the Chad Basin. These data supported the reconstruction of a L3f phylogenetic tree and calculation of times to the most recent common ancestor for all internal clades. A date ~8,000 YBP was estimated for the L3f3 sub-haplogroup, which is in good agreement with the supposed migration of Chadic speaking pastoralists and their linguistic differentiation from other Afro-Asiatic groups of East Africa. As a whole, the Afro-Asiatic language family presents low population structure, as 92.4% of mtDNA variation is found within populations and only 3.4% of variation can be attributed to diversity among language branches. The Chadic speaking populations form a relatively homogenous cluster, exhibiting lower diversification than the other Afro-Asiatic branches (Berber, Semitic and Cushitic). The results of our study support an East African origin of mitochondrial L3f3 clade that is present almost exclusively within Chadic speaking people living in Chad Basin. Whole genome sequence-based dates show that the ancestral haplogroup L3f must have emerged soon after the Out-of-Africa migration (around 57,100 +/- 9,400 YBP), but the "Chadic" L3f3 clade has much less internal variation, suggesting an expansion during the Holocene period about 8,000 +/- 2,500 YBP. This time period in the Chad Basin is known to have been particularly favourable for the expansion of pastoralists coming from northeastern Africa, as suggested by archaeological, linguistic and climatic data.

CZO perspective in Central Africa : The Lopé watershed, Lopé National Park, Ogooué River basin, Gabon.

NASA Astrophysics Data System (ADS)

Braun, J. J.; Jeffery, K.; Koumba Pambo, A. F.; Paiz, M. C.; Richter, D., Jr.; John, P.; Jerome, G.

2015-12-01

Critical Zone Observatories (CZO) in equatorial regions are seldom (see e. g. http://www.czen.org/, USA and http://rnbv.ipgp.fr/, France). The equatorial zone of Central Africa is almost free of them with the exception of the CZO of the Upper Nyong river basin (organic-rich river on the lateritic plateau of South Cameroon; SO BVET, http://bvet.omp.obs-mip.fr/). On both sides of the Equator line, the Ogooué River Basin (215,000 km2) stretches on about 80% of the total area of Gabon and drains various geological and morpho-pedological contexts and feeds the sedimentation areas of the Central African passive margin (Guillochaux et al., 2014). The Upper Ogooué (up to Lambaréné) drains the stepped planation surface of the Congo craton while the Lower Ogooué drains Mesozoic and Cenozoic sedimentary terrains. The climate is equatorial (Pmean = 2500 mm/yr; Tmean = 26 °; %humidity > 80%). Continuous hydro-climatic chronicles exist for the period 1953-1974 (managed by ORSTOM, now IRD). The runoff at Lambaréné (92% of the basin area) is very high (714 mm/yr). With a rural density of 1 inhabitant/km2, it is one of the last largely pristine tropical forested ecosystems on the Planet. In addition, the basin will be, in the coming decades, the theatre of important anthropogenic changes (dams, agriculture, mining, urbanisation, …). However, a conservation plan with an ambitious sustainable development policy is set up. This plan articulates the environmental issues related to the emergence of the country. Because of these characteristics, the basin offers ideal conditions for studying the changes in equatorial region of hydro-climate, weathering/erosion regimes and regolith production based on morpho-pedological contexts and associated physical, chemical and biological processes. It is thus germane to launch an integrated CZO initiative at both regional scale and local scale. At the regional scale, we plan to reactivate some of the hydro-climatic stations located on the planation surface (Franceville, Ayem, and Lambaréné). At the local scale, we plan to set up a small experimental watershed on the Lopé stream draining the northern part of the Lopé National Park, which is covered by a mosaic of forest and savannah. The Ogooué CZO will be highly complementary to the Nyong CZO, Cameroon, and a major asset for the international community.

Fatal nosocomial spread of Crimean-Congo hemorrhagic fever with very short incubation period.

PubMed

Naderi, HamidReza; Sheybani, Fereshte; Bojdi, Amin; Khosravi, Nasrin; Mostafavi, Irandokht

2013-03-01

Crimean-Congo hemorrhagic fever is a tick-borne viral zoonosis with the potential of human-to-human transmission with case fatality rates from 3% to 50%. The incubation period depends on host, route of infection, and viral dose. Herein, we report a nosocomial spread of the disease in a hospital at Mashhad, northeastern Iran, with a very short incubation period for one of the secondary cases. The patient was a medical student who had a negligible contact with a Crimean-Congo hemorrhagic fever patient during his admission to the hospital. The time interval between the contact and the onset of symptoms was merely 20 hours. Unfortunately, he died within 1 week of exposure.

Application du processus de Markov sur les occurrences des précipitations journalières au Congo-Brazzaville

NASA Astrophysics Data System (ADS)

Samba, Gaston; Mpounza, Marcel

2005-11-01

This study deals with the daily precipitation occurrence during the rainy season in different locations in Congo-Brazzaville. The Markov-chain probability model has been used for defining probable dry and wet rainy sequences at each station, and transition probabilities between daily precipitations of two successive days. The main results are: in Congo, the dependence on the preceding day of the daily precipitations occurrence; the confirmation of two main climatic region (equatorial climate to the north and tropical wet to the south); the lack of coherence in the distribution of precipitations. To cite this article: G. Samba, M. Mpounza, C. R. Geoscience 337 (2005).

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

Billerud signed a contract recently with the Societe d'Etudes de la Celulose du Congo (SECC) to conduct a feasibility study for a projected eucalyptus-based pulp plant for the production of bleached marketing pulp in The People's Republic of The Congo. SECC is a consortium of financial institutions, the Congolese government and Billerud. The Congolese government has been planning for the construction of a eucalyptus-based pulp plant for a long time, and in 1978, full-scale eucalyptus plantations were started. These plantations are based on fast growing hybrid species of eucalyptus developed by the Centre Technique Forestier Tropical in the Congo andmore » are ready for harvesting after only about seven years growing time.« less

New Foci of Buruli Ulcer, Angola and Democratic Republic of Congo

PubMed Central

Kibadi, Kapay; Panda, Mbutu; Tamfum, Jean-Jacques Muyembe; Fraga, Alexandra G.; Filho, Adhemar Longatto; Anyo, Gladys; Pedrosa, Jorge; Nakazawa, Yoshinori; Suykerbuyk, Patrick; Meyers, Wayne M.

2008-01-01

We report 3 patients with laboratory-confirmed Buruli ulcer in Kafufu/Luremo, Angola, and Kasongo-Lunda, Democratic Republic of Congo. These villages are near the Kwango/Cuango River, which flows through both countries. Further investigation of artisanal alluvial mining as a risk factor for Buruli ulcer is recommended. PMID:18976574

A Holistic, Person-Centred Care Model for Victims of Sexual Violence in Democratic Republic of Congo: The Panzi Hospital One-Stop Centre Model of Care.

PubMed

Mukwege, Denis; Berg, Marie

2016-10-01

Denis Mukwege and Marie Berg describe the One Stop Centre at Panzi Hospital in Eastern Democratic Republic of Congo that provides care for girls and women who have been raped in combination with extreme bodily harm.

New strategy for peace enforcement: the intervention brigade in the Democratic Republic of Congo

DTIC Science & Technology

With the introduction of MONUSCO’s Force Intervention Brigade in the Democratic Republic of Congo (DRC), the United Nations (UN), for the first time...can reduce risk by rebalancing its strategy or by separating the intervention brigade’s offensive mission from MONUSCO’s defensive one .

Media composition and incubation temperature affect Congo red dye affinity of Shiga toxin-producing Escherichia coli

USDA-ARS?s Scientific Manuscript database

Background: Escherichia coli biofilm formation is dependent on curli fimbriae and cellulose, and the expression of both varies among Shiga toxin-producing E. coli (STEC). Curli and cellulose expression are often identified by their affinity for Congo red dye (CR) but media composition and incubation...

Congo: Elections and the Battle for Mineral Resources

ERIC Educational Resources Information Center

Social Education, 2007

2007-01-01

Twenty million voters cast ballots July 30, 2006, in the Democratic Republic of the Congo's first free election since 1960. A runoff election three months later, between transitional president Joseph Kabila and transitional vice president Jean-Pierre Bemba, gave Kabila a mandate to lead the war-torn nation for five more years. The elections, in…

The Influence of Colonial Ideology on Schoolbooks in the Belgian Congo.

ERIC Educational Resources Information Center

Mbandaka, Honore Vinck

1995-01-01

An analysis of 50 textbooks used in the elementary schools of the former Belgian Congo reveals an overt attempt to propagate colonial ideology. Fundamental themes included the legitimacy of the colonization, denigration of the indigenous culture, and establishment of colonial authority. Three books, however, resisted this indoctrination and one…

Teaching and Supporting Students with Intellectual Disabilities in Democratic Republic of Congo

ERIC Educational Resources Information Center

Aldersey, Heather Michelle; Haines, Shana Jackson

2018-01-01

Children with intellectual disabilities (ID) often experience challenges due to low learning expectations and educational quality and limited educational access. Often, in locations impacted by higher levels of poverty, such as Kinshasa, Democratic Republic of the Congo, educational disparities for children with ID are even more pronounced. To…

Rights of the Child in the Democratic Republic of the Congo.

ERIC Educational Resources Information Center

Cosandey, Olivier

This report to the United Nations Convention on the Rights of the Child contains observations of the World Organisation Against Torture (OMCT) concerning the application of the Convention by Democratic Republic of the Congo (DRC). The report's introductory summary asserts that although the DRC has ratified the Convention and other international…

Albumin binds self-assembling dyes as specific polymolecular ligands.

PubMed

Stopa, Barbara; Rybarska, Janina; Drozd, Anna; Konieczny, Leszek; Król, Marcin; Lisowski, Marek; Piekarska, Barbara; Roterman, Irena; Spólnik, Paweł; Zemanek, Grzegorz

2006-12-15

Self-assembling dyes with a structure related to Congo red (e.g. Evans blue) form polymolecular complexes with albumin. The dyes, which are lacking a self-assembling property (Trypan blue, ANS) bind as single molecules. The supramolecular character of dye ligands bound to albumin was demonstrated by indicating the complexation of dye molecules outnumbering the binding sites in albumin and by measuring the hydrodynamic radius of albumin which is growing upon complexation of self-assembling dye in contrast to dyes lacking this property. The self-assembled character of Congo red was also proved using it as a carrier introducing to albumin the intercalated nonbonding foreign compounds. Supramolecular, ordered character of the dye in the complex with albumin was also revealed by finding that self-assembling dyes become chiral upon complexation. Congo red complexation makes albumin less resistant to low pH as concluded from the facilitated N-F transition, observed in studies based on the measurement of hydrodynamic radius. This particular interference with protein stability and the specific changes in digestion resulted from binding of Congo red suggest that the self-assembled dye penetrates the central crevice of albumin.

Accelerated decolorization of azo dye Congo red in a combined bioanode-biocathode bioelectrochemical system with modified electrodes deployment.

PubMed

Kong, Fanying; Wang, Aijie; Cheng, Haoyi; Liang, Bin

2014-01-01

In this study, BES with bioanode and biocathode was applied to decolorize an azo dye Congo red (CR). Results showed that the Congo red decolorization efficiency (CR-DE) within 23 h in a combined bioanode-biocathode single chamber BES was 98.3±1.3%, significantly higher than that of mixed solution in a dual chamber BES (67.2±3.5%) (P<0.005). Various electrodes deployments (horizontal, vertical and surrounding) in the combined bioanode-biocathode BES were further compared based on the decolorization performance and electrochemical characterization. Results indicated that CR-DE within 11h improved from 87.4±1.3% to 97.5±2.3%, meanwhile the internal resistance decreased from 236.6 to 42.2Ω as modifying the horizontal deployment to be a surrounding deployment. It proved that the combination of bioanode and biocathode with suitable electrodes deployment could accelerate the decolorization of azo dye Congo red, which would be great potential for the application of bioelectrochemical technology in azo dye wastewater treatment. Copyright © 2013 Elsevier Ltd. All rights reserved.

Tectonic inheritance in the development of the Kivu - north Tanganyika rift segment of the East African Rift System: role of pre-existing structures of Precambrian to early Palaeozoic origin.

NASA Astrophysics Data System (ADS)

Delvaux, Damien; Fiama Bondo, Silvanos; Ganza Bamulezi, Gloire

2017-04-01

The present architecture of the junction between the Kivu rift basin and the north Tanganyika rift basin is that of a typical accommodation zone trough the Ruzizi depression. However, this structure appeared only late in the development of the Western branch of the East African Rift System and is the result of a strong control by pre-existing structures of Precambrian to early Palaeozoic origin. In the frame of a seismic hazard assessment of the Kivu rift region, we (Delvaux et al., 2016) constructed homogeneous geological, structural and neotectonic maps cross the five countries of this region, mapped the pre-rift, early rift and Late Quaternary faults and compiled the existing knowledge on thermal springs (assumed to be diagnostic of current tectonic activity along faults). We also produced also a new catalogue of historical and instrumental seismicity and defined the seismotectonic characteristics (stress field, depth of faulting) using published focal mechanism data. Rifting in this region started at about 11 Ma by initial doming and extensive fissural basaltic volcanism along normal faults sub-parallel to the axis of the future rift valley, as a consequence of the divergence between the Nubia and the Victoria plate. In a later stage, starting around 8-7 Ma, extension localized along a series of major border faults individualizing the subsiding tectonic basins from the uplifting rift shoulders, while lava evolved towards alkali basaltic composition until 2.6 Ma. During this stage, initial Kivu rift valley was extending linearly in a SSW direction, much further than its the actual termination at Bukavu, into the Mwenga-Kamituga graben, up to Namoya. The SW extremity of this graben was linked via a long oblique transfer zone to the central part of Lake Tanganyika, itself reactivating an older ductile-brittle shear zone. In the late Quaternary-early Holocene, volcanism migrated towards the center of the basin, with the development of the Virunga volcanic massif, and the Kivu-Ruzizi accommodation zone connected the northern half of the former Kivu rift basin to the northern extremity of the Tanganyika basin. This process was influenced by the highly heterogeneous basement, formed during a long geological history with a dominantly brittle structuration during the Pan-African. The local stress field revealed by earthquake focal mechanisms appears strongly influenced by this heterogeneous structure but also by the transition towards the Congo basin on the western side of the rift and towards the Tanzanian carton on its eastern side. Delvaux, D. et al., 2016. Journal of African Earth Sciences. doi: 10.1016/j.jafrearsci.2016.10.004

Sensitive Next-Generation Sequencing Method Reveals Deep Genetic Diversity of HIV-1 in the Democratic Republic of the Congo.

PubMed

Rodgers, Mary A; Wilkinson, Eduan; Vallari, Ana; McArthur, Carole; Sthreshley, Larry; Brennan, Catherine A; Cloherty, Gavin; de Oliveira, Tulio

2017-03-15

As the epidemiological epicenter of the human immunodeficiency virus (HIV) pandemic, the Democratic Republic of the Congo (DRC) is a reservoir of circulating HIV strains exhibiting high levels of diversity and recombination. In this study, we characterized HIV specimens collected in two rural areas of the DRC between 2001 and 2003 to identify rare strains of HIV. The env gp41 region was sequenced and characterized for 172 HIV-positive specimens. The env sequences were predominantly subtype A (43.02%), but 7 other subtypes (33.14%), 20 circulating recombinant forms (CRFs; 11.63%), and 20 unclassified (11.63%) sequences were also found. Of the rare and unclassified subtypes, 18 specimens were selected for next-generation sequencing (NGS) by a modified HIV-switching mechanism at the 5' end of the RNA template (SMART) method to obtain full-genome sequences. NGS produced 14 new complete genomes, which included pure subtype C ( n = 2), D ( n = 1), F1 ( n = 1), H ( n = 3), and J ( n = 1) genomes. The two subtype C genomes and one of the subtype H genomes branched basal to their respective subtype branches but had no evidence of recombination. The remaining 6 genomes were complex recombinants of 2 or more subtypes, including subtypes A1, F, G, H, J, and K and unclassified fragments, including one subtype CRF25 isolate, which branched basal to all CRF25 references. Notably, all recombinant subtype H fragments branched basal to the H clade. Spatial-geographical analysis indicated that the diverse sequences identified here did not expand globally. The full-genome and subgenomic sequences identified in our study population significantly increase the documented diversity of the strains involved in the continually evolving HIV-1 pandemic. IMPORTANCE Very little is known about the ancestral HIV-1 strains that founded the global pandemic, and very few complete genome sequences are available from patients in the Congo Basin, where HIV-1 expanded early in the global pandemic. By sequencing a subgenomic fragment of the HIV-1 envelope from study participants in the DRC, we identified rare variants for complete genome sequencing. The basal branching of some of the complete genome sequences that we recovered suggests that these strains are more closely related to ancestral HIV-1 strains than to previously reported strains and is evidence that the local diversification of HIV in the DRC continues to outpace the diversity of global strains decades after the emergence of the pandemic. Copyright © 2017 Rodgers et al.

Multiple plant-wax compounds record differential sources and ecosystem structure in large river catchments

NASA Astrophysics Data System (ADS)

Hemingway, Jordon D.; Schefuß, Enno; Dinga, Bienvenu Jean; Pryer, Helena; Galy, Valier V.

2016-07-01

The concentrations, distributions, and stable carbon isotopes (δ13C) of plant waxes carried by fluvial suspended sediments contain valuable information about terrestrial ecosystem characteristics. To properly interpret past changes recorded in sedimentary archives it is crucial to understand the sources and variability of exported plant waxes in modern systems on seasonal to inter-annual timescales. To determine such variability, we present concentrations and δ13C compositions of three compound classes (n-alkanes, n-alcohols, n-alkanoic acids) in a 34-month time series of suspended sediments from the outflow of the Congo River. We show that exported plant-dominated n-alkanes (C25-C35) represent a mixture of C3 and C4 end members, each with distinct molecular distributions, as evidenced by an 8.1 ± 0.7‰ (±1σ standard deviation) spread in δ13C values across chain-lengths, and weak correlations between individual homologue concentrations (r = 0.52-0.94). In contrast, plant-dominated n-alcohols (C26-C36) and n-alkanoic acids (C26-C36) exhibit stronger positive correlations (r = 0.70-0.99) between homologue concentrations and depleted δ13C values (individual homologues average ⩽-31.3‰ and -30.8‰, respectively), with lower δ13C variability across chain-lengths (2.6 ± 0.6‰ and 2.0 ± 1.1‰, respectively). All individual plant-wax lipids show little temporal δ13C variability throughout the time-series (1σ ⩽ 0.9‰), indicating that their stable carbon isotopes are not a sensitive tracer for temporal changes in plant-wax source in the Congo basin on seasonal to inter-annual timescales. Carbon-normalized concentrations and relative abundances of n-alcohols (19-58% of total plant-wax lipids) and n-alkanoic acids (26-76%) respond rapidly to seasonal changes in runoff, indicating that they are mostly derived from a recently entrained local source. In contrast, a lack of correlation with discharge and low, stable relative abundances (5-16%) indicate that n-alkanes better represent a catchment-integrated signal with minimal response to discharge seasonality. Comparison to published data on other large watersheds indicates that this phenomenon is not limited to the Congo River, and that analysis of multiple plant-wax lipid classes and chain lengths can be used to better resolve local vs. distal ecosystem structure in river catchments.

Lipase, protease, and biofilm as the major virulence factors in staphylococci isolated from acne lesions.

PubMed

Saising, Jongkon; Singdam, Sudarat; Ongsakul, Metta; Voravuthikunchai, Supayang Piyawan

2012-08-01

Staphylococci involve infections in association with a number of bacterial virulence factors. Extracellular enzymes play an important role in staphylococcal pathogenesis. In addition, biofilm is known to be associated with their virulence. In this study, 149 staphylococcal isolates from acne lesions were investigated for their virulence factors including lipase, protease, and biofilm formation. Coagulase-negative staphylococci were demonstrated to present lipase and protease activities more often than coagulase-positive staphylococci. A microtiter plate method (quantitative method) and a Congo red agar method (qualitative method) were comparatively employed to assess biofilm formation. In addition, biofilm forming ability was commonly detected in a coagulase-negative group (97.7%, microtiter plate method and 84.7%, Congo red agar method) more frequently than in coagulase-positive organisms (68.8%, microtiter plate method and 62.5%, Congo red agar method). This study clearly confirms an important role for biofilm in coagulasenegative staphylococci which is of serious concern as a considerable infectious agent in patients with acnes and implanted medical devices. The Congo red agar method proved to be an easy method to quickly detect biofilm producers. Sensitivity of the Congo red agar method was 85.54% and 68.18% and accuracy was 84.7% and 62.5% in coagulase-negative and coagulase-positive staphylococci, respectively, while specificity was 50% in both groups. The results clearly demonstrated that a higher percentage of coagulasenegative staphylococci isolated from acne lesions exhibited lipase and protease activities, as well as biofilm formation, than coagulase-positive staphylococci.

The effect of operational parameters on the photocatalytic degradation of Congo red organic dye using ZnO-CdS core-shell nano-structure coated on glass by Doctor Blade method.

PubMed

Habibi, Mohammad Hossein; Rahmati, Mohammad Hossein

2015-02-25

Photocatalytic degradation of Congo red was investigated using ZnO-CdS core-shell nano-structure coated on glass by Doctor Blade method in aqueous solution under irradiation. Field-emission scanning electron microscopy (FESEM) and X-ray diffraction (XRD) techniques were used for the morphological and structural characterization of ZnO-CdS core-shell nanostructures. XRD results showed diffractions of wurtzite zinc oxide core and wurtzite cadmium sulfide shell. FESEM results showed that nanoparticles are nearly hexagonal with an average diameter of about 50 nm. The effect of catalyst loading, UV-light irradiation time and solution pH on photocatalytic degradation of Congo red was studied and optimized values were obtained. Results showed that the employment of efficient photocatalyst and selection of optimal operational parameters may lead to complete decolorization of dye solutions. It was found that ZnO-CdS core-shell nano-structure is more favorable for the degradation of Congo red compare to pure ZnO or pure CdS due to lower electron hole recombination. The results showed that the photocatalytic degradation rate of Congo red is enhanced with increasing the content of ZnO up to ZnO(0.2 M)/CdS(0.075 M) which is reached 88.0% within 100 min irradiation. Copyright © 2014 Elsevier B.V. All rights reserved.

A model to predict when a cholera outbreak might hit the Congo

NASA Astrophysics Data System (ADS)

Schultz, Colin

2014-09-01

In 2011, as many as 600,000 people in 58 countries contracted cholera, with thousands succumbing to the disease. In most countries, cholera is rare. In others, like the Democratic Republic of the Congo, cholera is an endemic threat, always lurking in the background waiting for the right set of conditions to spark an outbreak.

Human African Trypanosomiasis Transmission, Kinshasa, Democratic Republic of Congo

PubMed Central

Diabakana, Philemon Mansinsa; Mesu, Victor Kande Betu Ku; Manzambi, Emile Zola; Ollivier, Gaelle; Asonganyi, Tazoacha; Cuny, Gerard; Grébaut, Pascal

2006-01-01

To investigate the epidemiology of human African trypanosomiasis (sleeping sickness) in Kinshasa, Democratic Republic of Congo, 2 entomologic surveys were conducted in 2005. Trypanosoma brucei gambiense and human-blood meals were found in tsetse fly midguts, which suggested active disease transmission. Vector control should be used to improve human African trypanosomiasis control efforts. PMID:17326955

Crimean-Congo Hemorrhagic Fever Virus, Greece

PubMed Central

Sidira, Persefoni; Larichev, Victor; Gavrilova, Ludmila; Kuzmina, Ksenia; Mousavi-Jazi, Mehrdad; Mirazimi, Ali; Ströher, Ute; Nichol, Stuart

2014-01-01

Seroprevalence of Crimean-Congo hemorrhagic fever virus (CCHFV) is high in some regions of Greece, but only 1 case of disease has been reported. We used 4 methods to test 118 serum samples that were positive for CCHFV IgG by commercial ELISA and confirmed the positive results. A nonpathogenic or low-pathogenicity strain may be circulating. PMID:24447877

New Information and Communication Technologies in the Democratic Republic of Congo: Strategies and Measures

ERIC Educational Resources Information Center

Nsomwe-a-nfunkwa, Banza

2006-01-01

This article will primarily allow a definition of the strategic development of ICT in the Democratic Republic of Congo, to be put forward. For the most part it involves the precision of many types of measures and strategies (such as institutional, regulatory, infrastructural measures), human resources, the development of content and partnerships,…

Unique songs of African wood-owls (Strix woodfordii) in the Democratic Republic of Congo.

Treesearch

B.G. Marcot

2007-01-01

Statistical analysis of African wood-owl (Strix woodfordii) song spectrograms suggest a significantly different song type in Democratic Republic of Congo (DRC), central Africa, than elsewhere in eastern or southern Africa. Songs of DRC owls tend to be consistently shorter in duration and more monotone in overall frequency range. The first note is...

Poliomyelitis Outbreak,Pointe-Noire, Republic of the Congo, September 2010–February 2011

PubMed Central

Llosa, Augusto E.; Mouniaman-Nara, Isabelle; Kouassi, Felix; Ngala, Joseph; Boxall, Naomi; Porten, Klaudia; Grais, Rebecca F.

2011-01-01

On November 4, 2010, the Republic of the Congo declared a poliomyelitis outbreak. A cross-sectional survey in Pointe-Noire showed poor sanitary conditions and low vaccination coverage (55.5%), particularly among young adults. Supplementary vaccination should focus on older age groups in countries with evidence of immunity gaps. PMID:21801636

Crimean-Congo Hemorrhagic Fever in Migrant Worker Returning from Oman to India, 2016.

PubMed

Yadav, Pragya D; Thacker, Sachin; Patil, Deepak Y; Jain, Rajlaxmi; Mourya, Devendra T

2017-06-01

In January 2016, a migrant worker who returned home to India after becoming ill in Oman was confirmed to have Crimean-Congo hemorrhagic fever (CCHF). Physicians should include CCHF in the differential diagnosis for patients with hemorrhagic signs and a history of recent travel to any area where CCHF is endemic or prevalent.

Factors Affecting Performance of Agricultural Extension: Evidence from Democratic Republic of Congo

ERIC Educational Resources Information Center

Ragasa, Catherine; Ulimwengu, John; Randriamamonjy, Josee; Badibanga, Thaddee

2016-01-01

Purpose: As part of the institutional reforms and agricultural restructuring in the Democratic Republic of Congo (DRC), this paper provides an assessment of the performance of the agricultural extension system as well as factors explaining it. Method: This paper involves key informants' interviews and surveys of 107 extension organizations and 162…

Intellectual and Developmental Disabilities in Kinshasa, Democratic Republic of the Congo: Causality and Implications for Resilience and Support

ERIC Educational Resources Information Center

Aldersey, Heather M.; Turnbull, H. Rutherford, III; Turnbull, Ann P.

2014-01-01

This article reports results of a 7-month qualitative study on intellectual and related developmental disabilities in Kinshasa, Democratic Republic of the Congo, particularly as they relate to the causes and meaning of intellectual and developmental disabilities (IDD). This study raises important questions related to the understanding of…

An Examination of Indiscipline in Secondary Schools in the Congo.

ERIC Educational Resources Information Center

Bafoua, Justin

The purpose of this study was to: (1) investigate some of the causes of lack of discipline in secondary schools in the Congo Republic according to teachers, students, and parents; (2) look into the effects that discipline problems have on the students' performances and their development as responsible citizens and (3) suggest or recommend measures…

African Swine Fever Virus p72 Genotype IX in Domestic Pigs, Congo, 2009

PubMed Central

Anchuelo, Raquel; Pelayo, Virginia; Poudevigne, Frédéric; Leon, Tati; Nzoussi, Jacques; Bishop, Richard; Pérez, Covadonga; Soler, Alejandro; Nieto, Raquel; Martín, Hilario; Arias, Marisa

2011-01-01

African swine fever virus p72 genotype IX, associated with outbreaks in eastern Africa, is cocirculating in the Republic of the Congo with West African genotype I. Data suggest that viruses from eastern Africa are moving into western Africa, increasing the threat of outbreaks caused by novel viruses in this region. PMID:21801650

"New Education" for the Congo? The Indigenist Approach, New Education and Prescriptive Pedagogy

ERIC Educational Resources Information Center

Depaepe, Marc; Simon, Frank; Vinck, Honoré

2015-01-01

This article responds to a question put forward approximately a decade ago by the history of education research group at the Katholieke Universiteit Leuven: Did the child-centred ideas of New Education, as promoted by Ovide Decroly, influence the education policy in the former Belgian colony of Congo? Naturally, ideas were circulating that could…

76 FR 61134 - Waiver of Restriction on Assistance to the Central Government of the Democratic Republic of the...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-03

... DEPARTMENT OF STATE [Public Notice 7622] Waiver of Restriction on Assistance to the Central Government of the Democratic Republic of the Congo Pursuant to Section 7086(c)(2) of the Department of State... respect to the Democratic Republic of the Congo and I hereby waive such restriction. This determination...

Enlightenment and the "Heart of Darkness": (Neo)Imperialism in the Congo, and Elsewhere

ERIC Educational Resources Information Center

Stronach, Ian

2006-01-01

This article approaches the current state of qualitative inquiry by constructing an allegory of neo-imperialism. It is based substantively on a history and contemporary anthro-politics of the Congo and in particular the city of Kisangani; metaphorically on Conrad's unsettling deployment of that same place as "the heart of darkness"; and…

Conflict Minerals in Electronic Systems: An Overview and Critique of Legal Initiatives.

PubMed

Jameson, N Jordan; Song, Xin; Pecht, Michael

2016-10-01

The Democratic Republic of Congo has vast natural resources, many of which are regularly exploited by the electronics industry. Unfortunately, in addition to these resources, there are widespread human rights abuses committed by armed groups entrenched in the eastern part of the Democratic Republic of Congo. These armed groups are using profits from these minerals as a source of funding. Their human rights abuses have led to a growing humanitarian interest in the region and prompted the international community to action. This paper explores the conflicts in the Democratic Republic of Congo, provides an understanding of the link between human rights abuses and conflict minerals, and interprets and critiques the legal actions of the international community.

Quantifying drag on wellbore casings in moving salt sheets

NASA Astrophysics Data System (ADS)

Weijermars, R.; Jackson, M. P. A.; Dooley, T. P.

2014-08-01

Frontier hydrocarbon development projects in the deepwater slopes of the Gulf of Mexico Basin, Santos Basin and Lower Congo Basin all require wells to cross ductile layers of autochthonous or allochthonous salt moving at peak rates of 100 mm yr-1. The Couette-Poiseuille number is introduced here to help pinpoint the depth of shear stress reversal in such salt layers. For any well-planned through salt, the probable range of creep forces of moving salt needs to be taken into account when designing safety margins and load-factor tolerance of the well casing. Drag forces increase with wellbore diameter, but more significantly with effective viscosity and speed of the creeping salt layer. The potential drag forces on cased wellbores in moving salt sheets are estimated analytically using a range of salt viscosities (1015-1019 Pa s) and creep rates (0-10 mm yr-1). Drag on perfectly rigid casing of infinite strength may reach up to 13 Giga Newton per meter wellbore length in salt having a viscosity of 1019 Pa s. Well designers may delay stress accumulations due to salt drag when flexible casing accommodates some of the early displacement and strain. However, all creeping salt could displace, fracture and disconnect well casing, eventually. The shear strength of typical heavy duty well casing (about 1000 MPa) can be reached due to drag by moving salt. Internal flow of salt will then fracture the casing near salt entry and exit points, but the structural damage is likely to remain unnoticed early in the well-life when the horizontal shift of the wellbore is still negligibly small (at less than 1 cm yr-1). Disruption of casing and production flow lines within the anticipated service lifetime of a well remains a significant risk factor within distinct zones of low-viscosity salt which may reach ultrafast creep rates of 100 mm yr-1.

When chemoclines collapse.

NASA Astrophysics Data System (ADS)

Junium, C. K.; Uveges, B. T.; Scholz, C. A.

2016-12-01

OAEs have defied our attempts to apply modern, anoxic basin analogs such as the Black Sea. Modern and recent systems provide resolution that is unavailable in the geologic record. Meromictic lakes such as Lake Kivu in East Africa provide unique opportunities to explore the relationship between persistently anoxic conditions at depth and a wide range of proxies that are pertinent to the study of OAEs. Here we explore new organic geochemical and stable isotopic records from Lake Kivu in the East African Rift Basin of Rwanda. Lake Kivu is a stratified half-graben rift basin that lies between Rwanda and the Democratic Republic of Congo on the western arm of the African Rift. The deep waters of Lake Kivu are charged with significant quantities of carbon dioxide and methane and are thought to have episodically overturned in the past, a process which is indicated by pronounced brown sapropel layers that have organic carbon contents that can be higher than 30 wt. %. The upwelling of 13C-depleted methane and carbon dioxide induced an 8‰ decrease in the δ13C of organic carbon. Overturning also induced significant eutrophication through the delivery of ammonium and phosphate from deep waters. Under these conditions unimodal communities of diatoms proliferated and pigment biomarker records indicate a diverse suite of chlorophylls produced by phototrophic sulfide oxidizers. The δ15N of bulk sediments decrease from +2 to -2‰ and then increase to +8‰ over the span of 2 cm core depth. This dramatic swing was likely the result of infusion of ammonium into surface waters, fostering uptake fractionation and driving 15N-depletion. The subsequent increase in δ15N was the result of the coupling of nitrification and denitrification as ammonium stocks were depleted. Stable isotopic trends such as these from Lake Kivu are uncommon in the geochemical records of OAEs, but bear similarities to that of the Toarcian and suggests a similar mechanism.

Seroepidemiology of Selected Arboviruses in Febrile Patients Visiting Selected Health Facilities in the Lake/River Basin Areas of Lake Baringo, Lake Naivasha, and Tana River, Kenya

PubMed Central

Lwande, Olivia; Orindi, Benedict; Irura, Zephania; Ongus, Juliette; Sang, Rosemary

2015-01-01

Abstract Introduction: Arboviruses cause emerging and re-emerging infections affecting humans and animals. They are spread primarily by blood-sucking insects such as mosquitoes, ticks, midges, and sandflies. Changes in climate, ecology, demographic, land-use patterns, and increasing global travel have been linked to an upsurge in arboviral disease. Outbreaks occur periodically followed by persistent low-level circulation. Aim: This study was undertaken to determine the seroepidemiology of selected arboviruses among febrile patients in selected lake/river basins of Kenya. Methods: Using a hospital-based cross-sectional descriptive survey, febrile patients were recruited and their serum samples tested for exposure to immunoglobulin M (IgM) and IgG antibodies against Crimean–Congo hemorrhagic fever virus (CCHFV), Rift Valley fever virus (RVFV), West Nile virus (WNV), and chikungunya virus (CHIKV). Samples positive for CHIKV and WNV were further confirmed by the plaque reduction neutralization test (PRNT). Results: Of the 379 samples examined, 176 were IgG positive for at least one of these arboviruses (46.4%, 95% confidence interval [CI] 41.4–51.5%). Virus-specific prevalence for CCHF, RVF, WN, and CHIK was 25.6%, 19.5%, 12.4%, and 2.6%, respectively. These prevalences varied significantly with geographical site (p<0.001), with Tana recording the highest overall arboviral seropositivity. PRNT results for Alphaviruses confirmed that the actual viruses circulating in Baringo were Semliki Forest virus (SFV) and CHIKV, o'nyong nyong virus (ONNV) in Naivasha, and SFV and Sindbis virus (SINDV) in Tana delta. Among the flaviviruses tested, WNV was circulating in all the three sites. Conclusion: There is a high burden of febrile illness in humans due to CCHFV, RVFV, WNV, and CHIKV infection in the river/lake basin regions of Kenya. PMID:25700043

Ethnicity, Education, and Fertility Transition in Kinshasa, Congo. Working Paper 2-97-1. Revised.

ERIC Educational Resources Information Center

Shapiro, David; Tambashe, B. Oleko

Substantial ethnic differences in fertility were documented in the Congo in the mid-1950s. These differences, apparent as well among women residing in Kinshasa, the capital, were linked to variations across ethnic groups in the incidence of venereal diseases and sterility. By the mid-1970s ethnic differences in fertility had diminished but were…

Strategic Implications of Piracy in Benin’s Territorial Waters

DTIC Science & Technology

2013-03-01

requires that all stakeholders, political leaders, international organizations and the private sector work together to efficiently mitigate or...international partner. The GGC is composed of the eight countries of Angola, Cameroon, Gabon, Equatorial-Guinea, Nigeria , Republic of Congo, Democratic...environment complicated by territorial disputes exists between Angola and the Democratic Republic of Congo,14 between the Republic of Nigeria and the

Genetic Detection and Isolation of Crimean-Congo hemorrhagic fever virus, Kosovo, Yugoslavia

PubMed Central

Boźović, Bojana; Pavlidou, Vassiliki; Papadimitriou, Evangelia; Pelemis, Mijomir; Antoniadis, Aantonis

2002-01-01

Crimean-Congo hemorrhagic fever virus (C-CHFV) strains were isolated from a fatal case and the attending physician in Kosovo, Yugoslavia. Early, rapid diagnosis of the disease was achieved by reverse transcription-polymerase chain reaction. The physician was successfully treated with oral ribavirin. These cases yielded the first genetically studied C-CHFV human isolates in the Balkans. PMID:12141973

Interview with Jacques Bwira Hope Primary School Kampala, Uganda

ERIC Educational Resources Information Center

Harvard Educational Review, 2009

2009-01-01

Jacques Bwira arrived in Uganda in 2000, having fled the violent conflict in his native country, the Democratic Republic of Congo. Though he had trained and worked as a teacher in Congo, he feared that speaking only French would prevent him from making a living in his new home. The police officer who interrogated Jacques on arrival in the capital…

78 FR 76698 - Waiver of Restriction on Assistance to the Central Government of the Democratic Republic of the...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-18

... DEPARTMENT OF STATE [Public Notice 8552] Waiver of Restriction on Assistance to the Central Government of the Democratic Republic of the Congo Pursuant to Section 7031(b)(3) of the Department of State... similar provisions of law in prior year Acts with respect to the Democratic Republic of the Congo and I...

Ralph Bunche's International Legacy: The Middle East, Congo, and United Nations Peacekeeping

ERIC Educational Resources Information Center

Lyman, Princeton N.

2004-01-01

Ralph Bunche is remembered most for three major achievements in the international field. His mediation of the end of the first Israel-Arab war, for which he won the Nobel Peace Prize; his work in the tumultuous period of independence in the Congo; and his "invention" of United Nations peacekeeping, which itself won the Nobel Peace Prize…

Determinants and Consequences of Young Women's Access to Education in Kinshasa, Congo. Final Report to the Small Grants Program.

ERIC Educational Resources Information Center

Shapiro, David

A study analyzed data from a series of five household socioeconomic surveys carried out in Kinshasa, Congo, over the period from 1955 to 1990. Results indicated that schooling and educational attainment of both females and males increased substantially over this period, and particularly so for young women. Research analysis provides a detailed…

Are the Rural Schools of the Democratic Republic of Congo Ready for the $100 Laptop?

ERIC Educational Resources Information Center

Banza, Nsomwe-a- Nfunkwa

2006-01-01

The situation of schools in the Democratic Republic of Congo in matters concerning new information and communication technologies remains alarming. Given the primary role of these technologies in teaching and learning, as well as the concern of giving Congolese rural schools access to these tools, considering the problems of lack of electricity,…

76 FR 63935 - Notification of the Removal of Conditions of Entry on Vessels Arriving From the Republic of Congo

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-14

... arriving from the country of the Republic of Congo. DATES: The policy announced in this notice is effective... published a Notice of Policy in the Federal Register, (74 FR 45230), announcing that it had determined that... entry announced in the previously published Notice of Policy. With this notice, the current list of...

Preoperative determination of appropriate cutting line for proximal gastrectomy to avoid postoperative jejunal ulcer.

PubMed

Takahashi, Naoto; Kashimura, Hirotaka; Nimura, Hiroshi; Watanabe, Atsushi; Yano, Kentaro; Aoki, Hiroaki; Koyama, Tomoki; Sasaki, Toshiyuki; Shida, Atsuo; Mitsumori, Norio; Aoki, Teruaki; Kashiwagi, Hideyuki; Yanaga, Katsuhiko

2012-01-01

Although proximal gastrectomy has become a procedure of choice for patients' early cancer in the upper third of stomach, no clinical guide for optimal gastric resection in order to avoid postoperative jejunal ulcer is available. The aim of this study was to investigate whether determining the distribution of parietal and chief cells of the stomach using Congo red test is clinically relevant. The F-line was defined as a boundary line between fundic and intermediate area of the stomach according to the pathological findings in 29 patients who underwent total gastrectomy for early gastric cancer, whereas the f-line was regarded as a boundary line between intermediate and pyloric area. In the additional 6 patients undergoing vagus-preserving proximal gastrectomy with jejunal pouch interposition, endoscopic Congo red test was preoperatively performed to determine the F-f-line. The distances from the pyloric ring to f-line on the lesser and greater curvatures were variable. Long-term outcomes of proximal gastrectomy guided by preoperative endoscopic Congo red test were favorable. It is suggested that preoperative endoscopic Congo red test is useful to determine the appropriate cutting line in order to avoid postoperative jejunal ulcer after proximal gastrectomy.

Relative Abundance and Diversity of Bacterial Methanotrophs at the Oxic-Anoxic Interface of the Congo Deep-Sea Fan.

PubMed

Bessette, Sandrine; Moalic, Yann; Gautey, Sébastien; Lesongeur, Françoise; Godfroy, Anne; Toffin, Laurent

2017-01-01

Sitting at ∼5,000 m water depth on the Congo-Angola margin and ∼760 km offshore of the West African coast, the recent lobe complex of the Congo deep-sea fan receives large amounts of fluvial sediments (3-5% organic carbon). This organic-rich sedimentation area harbors habitats with chemosynthetic communities similar to those of cold seeps. In this study, we investigated relative abundance, diversity and distribution of aerobic methane-oxidizing bacteria (MOB) communities at the oxic-anoxic interface of sedimentary habitats by using fluorescence in situ hybridization and comparative sequence analysis of particulate mono-oxygenase ( pmoA ) genes. Our findings revealed that sedimentary habitats of the recent lobe complex hosted type I and type II MOB cells and comparisons of pmoA community compositions showed variations among the different organic-rich habitats. Furthermore, the pmoA lineages were taxonomically more diverse compared to methane seep environments and were related to those found at cold seeps. Surprisingly, MOB phylogenetic lineages typical of terrestrial environments were observed at such water depth. In contrast, MOB cells or pmoA sequences were not detected at the previous lobe complex that is disconnected from the Congo River inputs.

Production and optimization of bamboo hydrochars for adsorption of Congo red and 2-naphthol.

PubMed

Li, Yin; Meas, Arun; Shan, Shengdao; Yang, Ruiqin; Gai, Xikun

2016-05-01

Twelve hydrochars were produced from bamboo sawdust for adsorption of Congo red and 2-naphthol. The bamboo hydrochars have Brunauer-Emmett-Teller (BET) surface areas ranging from 2.63m(2)/g to 43.07m(2)/g, average pore diameters from 3.05nm to 3.83nm, pore volumes between 0.02cm(3)/g and 0.53cm(3)/g, and the surfaces of the hydrochars have diverse functional groups. The physico-chemical properties of the hydrochars critically depend on the hydrothermal conditions. All the hydrochars can adsorb Congo red and 2-naphthol from aqueous solutions, the largest adsorption capacity for Congo red is 33.7mg/g at the equilibrium concentration of 0.1mg/mL at 25°C, and the highest adsorption amount for 2-naphthol is 12.2mg/g at 25°C and 0.1mg/mL. Freundlich model can describe the adsorption isotherms of the both adsorbates slightly better than Langmuir model. These results provide a reference to the production and use of hydrochars as potential adsorbents in wastewater treatment. Copyright © 2016 Elsevier Ltd. All rights reserved.

Force-field parametrization and molecular dynamics simulations of Congo red

NASA Astrophysics Data System (ADS)

Król, Marcin; Borowski, Tomasz; Roterman, Irena; Piekarska, Barbara; Stopa, Barbara; Rybarska, Joanna; Konieczny, Leszek

2004-01-01

Congo red, a diazo dye widely used in medical diagnosis, is known to form supramolecular systems in solution. Such a supramolecular system may interact with various proteins. In order to examine the nature of such complexes empirical force field parameters for the Congo red molecule were developed. The parametrization of bonding terms closely followed the methodology used in the development of the charmm22 force field, except for the calculation of charges. Point charges were calculated from a fit to a quantum mechanically derived electrostatic potential using the CHELP-BOW method. Obtained parameters were tested in a series of molecular dynamics simulations of both a single molecule and a micelle composed of Congo red molecules. It is shown that newly developed parameters define a stable minimum on the hypersurface of the potential energy and crystal and ab initio geometries and rotational barriers are well reproduced. Furthermore, rotations around C-N bonds are similar to torsional vibrations observed in crystals of diphenyl-diazene, which confirms that the flexibility of the molecule is correct. Comparison of results obtained from micelles molecular dynamics simulations with experimental data shows that the thermal dependence of micelle creation is well reproduced.

Sparks creating light? Strengthening peripheral disease surveillance in the Democratic Republic of Congo

PubMed Central

Mossoko, M.; Nyakio Kakusu, J. P.; Nyembo, J.; Mangion, J. P.; Van Laeken, D.; Van den Bergh, R.; Van den Boogaard, W.; Manzi, M.; Kibango, W. K.; Hermans, V.; Beijnsberger, J.; Lambert, V.; Kitenge, E.

2016-01-01

Setting: The Democratic Republic of Congo suffers from an amalgam of disease outbreaks and other medical emergencies. An efficient response to these relies strongly on the national surveillance system. The Pool d'Urgence Congo (PUC, Congo Emergency Team) of Médecins Sans Frontières is a project that responds to emergencies in highly remote areas through short-term vertical interventions, during which it uses the opportunity of its presence to reinforce the local surveillance system. Objective: To investigate whether the ancillary strengthening of the peripheral surveillance system during short-term interventions leads to improved disease notification. Design: A descriptive paired study measuring disease notification before and after 12 PUC interventions in 2013–2014. Results: A significant increase in disease notification was observed after seven mass-vaccination campaigns and was sustained over 6 months. For the remaining five smaller-scaled interventions, no significant effects were observed. Conclusion: The observed improvements after even short-term interventions underline, on the one hand, how external emergency actors can positively affect the system through their punctuated actions, and, on the other hand, the dire need for investment in surveillance at peripheral level. PMID:27358796

DOE Office of Scientific and Technical Information (OSTI.GOV)

Brambilla, M.; Del Franco, E.; Koko, J.

The Kitina Field Exploitation Permit was granted in 1994 to the Joint Venture formed by Agip Recherches Congo (Operator), Hydro Congo and Chevron International Ltd. Congo for a duration of twenty years. The Kitina Field expected production is 55,000 BOPD. The Development Scheme consists of an eight legged fixed platform located Offshore Congo, in a water depth of 137m, and linked to an existing onshore Terminal in Djeno by a 65 km. long, 14 in. sealine. The platform design provides for simultaneous drilling, or workover, and production. Furthermore, wells tie-back by a Tender Assisted Rig and hookup activities will bemore » performed simultaneously, to optimize the Project Overall Schedule so anticipating First Oil Production. The design and technical solutions have been optimized to cope with high pour point and high paraffin content. An original contract configuration has been devised in order both to reduce the expected investment cost and to stimulate a joint effort to meet the Target Price. This is being achieved by the direct involvement of all parties in the Project`s economic results without hindering the plant operability, safety or quality. To that end, the Contract has been subdivided into three portions: Lump Sum, Reimbursable and Alliance between the Client and the Consortium.« less

Paleomagnetism of the 765 Ma Luakela Volcanics in NW Zambia and Implications for Neoproterozoic Positions of the Congo Craton

NASA Astrophysics Data System (ADS)

Wingate, M. T.; Pisarevsky, S. A.; de Waele, B.

2004-12-01

Owing to the scarcity of reliable paleopoles, the Neoproterozoic position of the Congo craton (incorporating the Sao Francisco, Tanzania, and Bangweulu blocks) is very poorly known. We report new paleomagnetic data for the 765 ± 5 Ma Luakela volcanics, a NE-trending belt of basaltic to andesitic flows in NW Zambia (Key et al., 2001, J. Afr. Earth Sci., 33, 503-528). The volcanics are up to 0.8 km thick and occur within a 2 km thick succession of siliciclastic rocks that unconformably overlies Neoarchean and Paleoproterozoic rocks of the Congo craton margin, and is correlated with the Roan and Mwashia Groups of the Katanga Supergroup (Key et al., 2001). The strata are essentially undeformed, and either subhorizontal or dip shallowly to the SE. Although no metamorphic mineral growth is observed in fine-grained sedimentary rocks, alteration has strongly affected plagioclase and pyroxene in the volcanic rocks, and magnetite has been partially altered to hematite (Key et al., 2001). AF and thermal analysis of 65 samples from nine sites isolated three magnetisation components. Component A, carried mainly by SD magnetite, is directed very shallowly to the SE. Component B, carried mainly by hematite, is oriented shallowly SW-up. A low stability component C is directed very steeply downward. Some samples contain only component A, others only component B, and some contain both A and B. Component A is likely to be primary, because it is carried by SD magnetite (which petrography indicates is primary), does not resemble younger magnetisations from the Congo craton, and because the rocks have not been thermally metamorphosed. Component B, carried by hematite, we consider to be an overprint, possibly acquired during Pan-African deformation in the Lufilian Arc. Component C is similar to Permo-Carboniferous paleodirections from the region, and may have been acquired at that time. Paleopoles for components A and B (LVA and LVB) are about 90° apart, and similar to those from the Tanzania block. LVA coincides with a reliable pole for the Mbozi complex (Meert et al., 1995, Precamb. Res., 69, 113-131), for which several K-Ar results are within uncertainty of a U-Pb age of 748 ± 6 Ma (Mbede et al., 2004, ICESA Conf: The East African Rift, Addis Ababa, PDF abst.). The LVA and Mbozi poles place Congo at the equator at 765-750 Ma. LVB falls within uncertainty of a pole for the Gagwe lavas (Meert et al., 1995), which have an Ar-Ar cooling age of 795 ± 7 Ma (Deblond et al., 2001, J. Afr. Earth Sci., 32, 435-449). LVB cannot be older than 765 Ma, however. If the Tanzania block has not moved significantly relative to the Congo craton since at least 800 Ma, either the 795 Ma age is incorrect, or the Gagwe pole represents a younger overprint. The latter possibly implies that models which invoke a 90° CCW rotation of Congo between 800 and 750 Ma are no longer supported. Instead, the Congo craton rotated in the opposite direction between 750 Ma and the time of component B acquisition. The Luakela volcanics are overlain by 200 m of siltstones, followed by an unknown thickness of poorly-exposed diamictite, correlated with the `Grand Conglomerat', a widespread glaciogenic unit of Sturtian age at the base of the Kundelungu Group. The diamictite is younger than the 765 Ma volcanics, and older than volcanic pods, dated at 735 Ma, in contact with the diamictite (Key et al., 2001). The Congo craton occupied equatorial latitudes at 765-750 Ma, suggesting that the diamictite, the `Grand Conglomerat', and other Sturtian glaciogenic rocks in the Congo craton, represent a low-latitude glaciation.

Human exposure to metals due to consumption of fish from an artificial lake basin close to an active mining area in Katanga (D.R. Congo).

PubMed

Squadrone, S; Burioli, E; Monaco, G; Koya, M K; Prearo, M; Gennero, S; Dominici, A; Abete, M C

2016-10-15

The concentrations of 14 essential and nonessential trace elements were determined in fish from Lake Tshangalele, Katanga province, Democratic Republic of Congo. This province has been a place of intensive mining activities for centuries, which have increased in recent years, due to the use of metals such as copper and cobalt for the industries of fast-growing countries. Lake Tshangalele, which receives effluents from metallurgical and mining plants in Likasi, is home to several fish species that are an important part of the diet of the local population, and, therefore, it constitutes a relevant site for documenting the human exposure to metals as a result of a fish diet. The highest concentrations (median levels, dry weight) of cobalt (7.25mgkg(-1)), copper (88.1mgkg(-1)), iron (197.5mgkg(-1)), manganese (65.35mgkg(-1)), zinc (122.9mgkg(-1)) and aluminum (135.4mgkg(-1)) were found in fish collected closest to the copper mining plant, with decreasing concentrations along the lake, up to the dam. In the most contaminated fish samples, values of up to 270.1mgkg(-1) for Al, 173.1mgkg(-1) for Cu, 220.9mgkg(-1) for Zn, 211.0mgkg(-1) for Mn, 324.2mgkg(-1) for Fe, 15.1mgkg(-1) for Co, 4.2mgkg(-1) for Cr, 1.6mgkg(-1) for Cd, 1.9mgkg(-1) for Pb, and 1.8mgkg(-1) for Ni were found. Metal contamination from mining activity resulted in being of great concern because of potential health risks to the local inhabitants due to the consumption of heavily contaminated fish. High levels of metals, especially cobalt, aluminum, iron, manganese, zinc and cadmium were found in fish from Tshangalele water system. Copyright © 2016 Elsevier B.V. All rights reserved.

Weak Learner Method for Estimating River Discharges using Remotely Sensed Data: Central Congo River as a Testbed

NASA Astrophysics Data System (ADS)

Kim, D.; Lee, H.; Yu, H.; Beighley, E.; Durand, M. T.; Alsdorf, D. E.; Hwang, E.

2017-12-01

River discharge is a prerequisite for an understanding of flood hazard and water resource management, yet we have poor knowledge of it, especially over remote basins. Previous studies have successfully used a classic hydraulic geometry, at-many-stations hydraulic geometry (AMHG), and Manning's equation to estimate the river discharge. Theoretical bases of these empirical methods were introduced by Leopold and Maddock (1953) and Manning (1889), and those have been long used in the field of hydrology, water resources, and geomorphology. However, the methods to estimate the river discharge from remotely sensed data essentially require bathymetric information of the river or are not applicable to braided rivers. Furthermore, the methods used in the previous studies adopted assumptions of river conditions to be steady and uniform. Consequently, those methods have limitations in estimating the river discharge in complex and unsteady flow in nature. In this study, we developed a novel approach to estimating river discharges by applying the weak learner method (here termed WLQ), which is one of the ensemble methods using multiple classifiers, to the remotely sensed measurements of water levels from Envisat altimetry, effective river widths from PALSAR images, and multi-temporal surface water slopes over a part of the mainstem Congo. Compared with the methods used in the previous studies, the root mean square error (RMSE) decreased from 5,089 m3s-1 to 3,701 m3s-1, and the relative RMSE (RRMSE) improved from 12% to 8%. It is expected that our method can provide improved estimates of river discharges in complex and unsteady flow conditions based on the data-driven prediction model by machine learning (i.e. WLQ), even when the bathymetric data is not available or in case of the braided rivers. Moreover, it is also expected that the WLQ can be applied to the measurements of river levels, slopes and widths from the future Surface Water Ocean Topography (SWOT) mission to be launched in 2021.

Evaluation of disease and viral biomarkers as triggers for therapeutic intervention in respiratory mousepox - an animal model of smallpox

PubMed Central

Parker, Scott; Chen, Nanhai G.; Foster, Scott; Hartzler, Hollyce; Hembrador, Ed; Hruby, Dennis; Jordan, Robert; Lanier, Randall; Painter, George; Painter, Wesley; Sagartz, John E.; Schriewer, Jill; Buller, R. Mark

2013-01-01

The human population is currently faced with the potential use of natural or recombinant variola and monkeypox viruses as biological weapons. Furthermore, the emergence of human monkeypox in Africa and its expanding environs poses a significant natural threat. Such occurrences would require therapeutic and prophylactic intervention with antivirals to minimize morbidity and mortality of exposed populations. Two orally-bioavailable antivirals are currently in clinical trials; namely CMX001, an ether-lipid analogue of cidofovir with activity at the DNA replication stage and ST-246, a novel viral egress inhibitor. Both of these drugs have previously been evaluated in the ectromelia/mousepox system; however, the trigger for intervention was not linked to a disease biomarker or a specific marker of virus replication. In this study we used lethal, intranasal, ectromelia virus infections of C57BL/6 and hairless SKH1 mice to model human disease and evaluate exanthematous rash (rash) as an indicator to initiate antiviral treatment. We show that significant protection can be provided to C57BL/6 mice by CMX001 or ST-246 when therapy is initiated on day 6 post infection or earlier. We also show that significant protection can be provided to SKH1 mice treated with CMX001 at day 3 post infection or earlier, but this is 4 or more days before detection of rash (ST-246 not tested). Although in this model rash could not be used as a treatment trigger, viral DNA was detected in blood by day 4 post infection and in the oropharyngeal secretions (saliva) by day 2-3 post infection – thus providing robust and specific markers of virus replication for therapy initiation. These findings are discussed in the context of current respiratory challenge animal models in use for the evaluation of poxvirus antivirals. PMID:22381921

Buccal viral DNA as a trigger for brincidofovir therapy in the mousepox model of smallpox.

PubMed

Crump, Ryan; Korom, Maria; Buller, R Mark; Parker, Scott

2017-03-01

Orthopoxviruses continue to pose a significant threat to the population as potential agents of bioterrorism. An intentional release of natural or engineered variola virus (VARV) or monkeypox viruses would cause mortality and morbidity in the target population. To address this, antivirals have been developed and evaluated in animal models of smallpox and monkeypox. One such antiviral, brincidofovir (BCV, previously CMX001), has demonstrated high levels of efficacy against orthopoxviruses in animal models and is currently under clinical evaluation for prevention and treatment of diseases caused by cytomegaloviruses and adenoviruses. In this study we use the mousepox model of smallpox to evaluate the relationship between the magnitude of the infectious virus dose and an efficacious BCV therapy outcome when treatment is initiated concomitant with detection of ectromelia virus viral DNA (vDNA) in mouse buccal swabs. We found that vDNA could be detected in buccal swabs of some, but not all infected mice over a range of challenge doses by day 3 or 4 postexposure, when initiation of BCV treatment was efficacious, suggesting that detection of vDNA in buccal swabs could be used as a trigger to initiate BCV treatment of an entire potentially exposed population. However, buccal swabs of some mice did not become positive until 5 days postexposure, when initiation of BCV therapy failed to protect mice that received high doses of virus. And finally, the data suggest that the therapeutic window for efficacious BCV treatment decreases as the virus infectious dose increases. Extrapolating these findings to VARV, the data suggest that treatment should be initiated as soon as possible after exposure and not rely on a diagnostic tool such as the measurement of vDNA in buccal cavity swabs; however, consideration should be given to the fact that the behavior/disease-course of VARV in humans is different from that of ectromelia virus in the mouse. Copyright © 2016 Elsevier B.V. All rights reserved.

Evaluation of disease and viral biomarkers as triggers for therapeutic intervention in respiratory mousepox - an animal model of smallpox.

PubMed

Parker, Scott; Chen, Nanhai G; Foster, Scott; Hartzler, Hollyce; Hembrador, Ed; Hruby, Dennis; Jordan, Robert; Lanier, Randall; Painter, George; Painter, Wesley; Sagartz, John E; Schriewer, Jill; Mark Buller, R

2012-04-01

The human population is currently faced with the potential use of natural or recombinant variola and monkeypox viruses as biological weapons. Furthermore, the emergence of human monkeypox in Africa and its expanding environs poses a significant natural threat. Such occurrences would require therapeutic and prophylactic intervention with antivirals to minimize morbidity and mortality of exposed populations. Two orally-bioavailable antivirals are currently in clinical trials; namely CMX001, an ether-lipid analog of cidofovir with activity at the DNA replication stage and ST-246, a novel viral egress inhibitor. Both of these drugs have previously been evaluated in the ectromelia/mousepox system; however, the trigger for intervention was not linked to a disease biomarker or a specific marker of virus replication. In this study we used lethal, intranasal, ectromelia virus infections of C57BL/6 and hairless SKH1 mice to model human disease and evaluate exanthematous rash (rash) as an indicator to initiate antiviral treatment. We show that significant protection can be provided to C57BL/6 mice by CMX001 or ST-246 when therapy is initiated on day 6 post infection or earlier. We also show that significant protection can be provided to SKH1 mice treated with CMX001 at day 3 post infection or earlier, but this is four or more days before detection of rash (ST-246 not tested). Although in this model rash could not be used as a treatment trigger, viral DNA was detected in blood by day 4 post infection and in the oropharyngeal secretions (saliva) by day 2-3 post infection - thus providing robust and specific markers of virus replication for therapy initiation. These findings are discussed in the context of current respiratory challenge animal models in use for the evaluation of poxvirus antivirals. Copyright © 2012 Elsevier B.V. All rights reserved.

APPLICATION OF HEAVY MEDIA SEPARATION, FLOTATION AND CARBONATE LEACHING TO CONGO ORES. Topical Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Breymann, J.B. III

1951-10-31

The possibility of separating the U minerals from the carbonate minerals in magnesitic-dolomitic U ores from Union Miniere du Haute Katanga, Belgian Congo, by heavy media separation or froth flotation was investigated. Tables are included which show conditions and reagents. Results of tests of carbonate leaching of the whole ore and of the carbonate fiotation products are also tabulated. (auth)

Women's Education, Employment, and Fertility in Kinshasa, Congo, 1955-1990: A Descriptive Overview. Working Paper 10-97-1.

ERIC Educational Resources Information Center

Shapiro, David

This report provides a descriptive overview of women's schooling and educational attainment, employment activity, and fertility behavior in Kinshasa, the capital of the Democratic Republic of Congo. Data were used from a series of five household surveys carried out between 1955 and 1990 to see how these variables have changed during this period.…

Cross-sectional Serosurvey of Crimean-Congo Hemorrhagic Fever Virus IgG in Livestock, India, 2013-2014.

PubMed

Mourya, Devendra T; Yadav, Pragya D; Shete, Anita M; Sathe, Padmakar S; Sarkale, Prasad C; Pattnaik, Bramhadev; Sharma, Gaurav; Upadhyay, Kamlesh J; Gosavi, Surekha; Patil, Deepak Y; Chaubal, Gouri Y; Majumdar, Triparna D; Katoch, Vishwa M

2015-10-01

We conducted a cross-sectional serosurvey of Crimean-Congo hemorrhagic fever (CCHF) among livestock in 22 states and 1 union territory of India. A total of 5,636 samples from bovines, sheep, and goats were screened for CCHF virus IgG. IgG was detected in 354 samples, indicating that this virus is widespread in this country.

Invasive Salmonella enterica Serotype Typhimurium Infections, Democratic Republic of the Congo, 2007–2011

PubMed Central

Ley, Benedikt; Le Hello, Simon; Lunguya, Octavie; Lejon, Veerle; Muyembe, Jean-Jacques; Jacobs, Jan

2014-01-01

Infection with Salmonella enterica serotype Typhimurium sequence type (ST) 313 is associated with high rates of drug resistance, bloodstream infections, and death. To determine whether ST313 is dominant in the Democratic Republic of the Congo, we studied 180 isolates collected during 2007–2011; 96% belonged to CRISPOL type CT28, which is associated with ST313. PMID:24655438

Conservation Education and Environmental Communication in Great Ape Re-Introduction Projects: Two Cases from the Republic of Congo

ERIC Educational Resources Information Center

Cartwright, Barbara J.; Wall, John E.; Kaya, J. A. Placide

2012-01-01

Among species recovery tools available, re-introduction of animals to the wild is one of the more complex. Since the mid-1990s two successful great ape re-introductions have taken place in the Republic of Congo, leading some conservationists to revisit re-introduction as a strategy. This research explored the role of conservation education and…

Public Expenditure Review of the Education Sector in the Democratic Republic of Congo: An Efficiency, Effectiveness, and Equity Analysis. Report No. ACS14542

ERIC Educational Resources Information Center

Bollag, Burton, Ed.

2015-01-01

A sound education sector is fundamental for the economic, social, and political transformation of the Democratic Republic of Congo (DRC). The DRC has achieved significant progress in its education sector over the last decade, demonstrating strong resilience following a particularly violent period in its history. The DRC's development trajectory…

Feasibility Analysis of an Evidence-Based Positive Prevention Intervention for Youth Living with HIV/AIDS in Kinshasa, Democratic Republic of the Congo

ERIC Educational Resources Information Center

Parker, L.; Maman, S.; Pettifor, A.; Chalachala, J. L.; Edmonds, A.; Golin, C. E.; Moracco, K.; Behets, F.

2013-01-01

We evaluated the feasibility of a Positive Prevention intervention adapted for youth living with HIV/AIDS (YLWH) ages 15-24 in Kinshasa, Democratic Republic of the Congo. We conducted in-depth interviews and focus group discussions with intervention facilitators and YLWH participants on the following four areas of a feasibility framework:…

Origin and distribution of the organic matter in the distal lobe of the Congo deep-sea fan - A Rock-Eval survey

NASA Astrophysics Data System (ADS)

Baudin, François; Stetten, Elsa; Schnyder, Johann; Charlier, Karine; Martinez, Philippe; Dennielou, Bernard; Droz, Laurence

2017-08-01

The Congo River, the second largest river in the world, is a major source of organic matter for the deep Atlantic Ocean because of the connection of its estuary to the deep offshore area by a submarine canyon which feeds a vast deep-sea fan. The lobe zone of this deep-sea fan is the final receptacle of the sedimentary inputs presently channelled by the canyon and covers an area of 2500 km². The quantity and the source of organic matter preserved in recent turbiditic sediments from the distal lobe of the Congo deep-sea fan were assessed using Rock-Eval pyrolysis analyses. Six sites, located at approximately 5000 m water-depth, were investigated. The mud-rich sediments of the distal lobe contain high amounts of organic matter ( 3.5 to 4% Corg), the origin of which is a mixture of terrestrial higher-plant debris, soil organic matter and deeply oxidized phytoplanktonic material. Although the respective contribution of terrestrial and marine sources of organic matter cannot be precisely quantified using Rock-Eval analyses, the terrestrial fraction is dominant according to similar hydrogen and oxygen indices of both suspended and bedload sediments from the Congo River and that deposited in the lobe complex. The Rock-Eval signature supports the 70% to 80% of the terrestrial fraction previously estimated using C/N and δ13Corg data. In the background sediment, the organic matter distribution is homogeneous at different scales, from a single turbiditic event to the entire lobe, and changes in accumulation rates only have a limited effect on the quantity and quality of the preserved organic matter. Peculiar areas with chemosynthetic bivalves and/or bacterial mats, explored using ROV Victor 6000, show a Rock-Eval signature similar to background sediment. This high organic carbon content associated to high sedimentation rates (> 2 to 20 mm.yr-1) in the Congo distal lobe complex implies a high burial rate for organic carbon. Consequently, the Congo deep-sea fan represents an enormous sink of terrestrial organic matter when compared to other turbiditic systems over the world.

The "fault of the Pool" along the Congo River between Kinshasa and Brazzaville, R(D)Congo is no more a myth: Paleostress from small-scale brittle structures

NASA Astrophysics Data System (ADS)

Delvaux, Damien; Ganza, Gloire; Kongota, Elvis; Fukiabantu, Guilain; Mbokola, Dim; Boudzoumou, Florent; Miyouna, Timothée; Gampio, Urbain; Nkodia, Hardy

2017-04-01

Small-scale brittle structures such as shear fractures and tension joints are well developed in the indurated Paleozoic Inkisi red sandstones of the West-Congo Supergroup in the "pool" region of Kinshasa and Brazzaville, along the Congo River. They appear to be related to the evolution of intraplate stresses during the late Cretaceous-Paleogene period, possibly related to the opening of the South Atlantic. However, inferring paleostresses from such structures is difficult due to the lack of clear kinematic indicators, so we used mainly the geometry, architecture and sequence of the joint systems to infer paleostresses. A limited number of kinematic indicators for slip sense (displaced pebbles, irregularities on striated surfaces, slickensides) or extension (plume joints) confirm the general conclusions of the joint architecture analysis. We found evidence for two major brittle deformation systems, leading to almost orthogonal fracture sets. They both started by the development of plume joints, which progressively evolved into open tension joints, isolated shear fractures and long (up to several hundred meters) brittle shear zones. The first system started to develop under NE-SW extension and evolved into strike-slip with NNW-SSE horizontal compression while the second (and later), started to develop under NW-SE extension and evolved into strike-slip with NNE-SSW horizontal compression. The second brittle deformation episode was associated with fluid flow as shown by the presence of palygorskite-calcite veins in the most prominent fractures of the second fracture system. Along the NE-SW brittle shear zones which run parallel to the Congo River, carbonate-rich fault-gauge lenses are filled by sand derived from the crushed adjacent walls and calcite vein fragments injected at a high fluid pressure, with late precipitation of palygorskite. Our study demonstrates the existence of two fault systems between Kinshasa and Brazzaville, the first one orthogonal to the trend of the Congo River and the second one, orthogonal to it. This reconciles the different views on the suspected presence of a major fault in the Pool.

Shallow plumbing systems inferred from spatial analysis of pockmark arrays

NASA Astrophysics Data System (ADS)

Maia, A.; Cartwright, J. A.; Andersen, E.

2016-12-01

This study describes and analyses an extraordinary array of pockmarks at the modern seabed of the Lower Congo Basin (offshore Angola), in order to understand the fluid migration routes and shallow plumbing system of the area. The 3D seismic visualization of feeding conduits (pipes) allowed the identification of the source interval for the fluids expelled during pockmark formation. Spatial statistics are used to show the relationship between the underlying (polarised) polygonal fault (PPFs) patterns and seabed pockmarks distributions. Our results show PPFs control the linear arrangement of pockmarks and feeder pipes along fault strike, but faults do not act as conduits. Spatial statistics also revealed pockmark occurrence is not considered to be random, especially at short distances to nearest neighbours (<200m) where anti-clustering distributions suggest the presence of an exclusion zone around each pockmark in which no other pockmark will form. The results of this study are relevant for the understanding of shallow fluid plumbing systems in offshore settings, with implications on our current knowledge of overall fluid flow systems in hydrocarbon-rich continental margins.

Hysteresis in the Central African Rainforest

NASA Astrophysics Data System (ADS)

Pietsch, Stephan Alexander; Elias Bednar, Johannes; Gautam, Sishir; Petritsch, Richard; Schier, Franziska; Stanzl, Patrick

2014-05-01

Past climate change caused severe disturbances of the Central African rainforest belt, with forest fragmentation and re-expansion due to drier and wetter climate conditions. Besides climate, human induced forest degradation affected biodiversity, structure and carbon storage of Congo basin rainforests. Information on climatically stable, mature rainforest, unaffected by human induced disturbances, provides means of assessing the impact of forest degradation and may serve as benchmarks of carbon carrying capacity over regions with similar site and climate conditions. BioGeoChemical (BGC) ecosystem models explicitly consider the impacts of site and climate conditions and may assess benchmark levels over regions devoid of undisturbed conditions. We will present a BGC-model validation for the Western Congolian Lowland Rainforest (WCLRF) using field data from a recently confirmed forest refuge, show model - data comparisons for disturbed und undisturbed forests under different site and climate conditions as well as for sites with repeated assessment of biodiversity and standing biomass during recovery from intensive exploitation. We will present climatic thresholds for WCLRF stability, analyse the relationship between resilience, standing C-stocks and change in climate and finally provide evidence of hysteresis.

Riverine dissolved lithium isotopic signatures in low-relief central Africa and their link to weathering regimes

NASA Astrophysics Data System (ADS)

Henchiri, Soufian; Gaillardet, Jérôme; Dellinger, Mathieu; Bouchez, Julien; Spencer, Robert G. M.

2016-05-01

The isotopic composition of dissolved lithium (δ7Li) near the Congo River mouth varied from 14‰ to 22‰ in 2010 and was negatively correlated to discharge. From the relationship between dissolved δ7Li and strontium isotopes, we suggest that this large variation is due to mixing of waters from two contrasting continental weathering regimes. One end-member (high δ7Li ≈ 25‰) represents waters sourced from active lateritic soils covering the periphery of the basin (Li highly sequestered into secondary mineral products) and another representing blackwater rivers (low δ7Li ≈ 5.7‰) derived from the swampy central depression where high organic matter content in water leads to congruent dissolution of the Tertiary sedimentary bedrock. This suggests that the lithium isotopic signature of tropical low-relief surfaces is not unique and traces the long-term, large-scale vertical motions of the continental crust that control geomorphological settings. This evolution should be recorded in the oceanic secular δ7Li curve.

New synonymies and new records of Afrotropical and Madagascan Pentatominae (Hemiptera: Heteroptera: Pentatomidae).

PubMed

Kment, Petr; Jindra, Zdeněk; Rider, David A

2014-09-24

The following new synonymies within the family Pentatomidae, subfamily Pentatominae, are established: Aesula Stål, 1876 = Pseudacrosternum Day, 1965, syn. nov. (Nezarini), Aesula viridissima (Signoret, 1861) = Aesula signoretiana Kirkaldy, 1909, syn. nov. = Pseudacrosternum cachani Day, 1965, syn. nov. = Pseudacrosternum cachani var. scutellatum Day, 1965, syn. nov., and Andocides vittaticeps (Stål, 1858) = Andocides vittaticeps var. viridescens Schumacher, 1913, syn. nov. Afrania brachyptera (Schaum, 1853), stat. restit., is restored as the oldest available name for that species, with Afrania wahlbergi Stål, 1854, syn. restit., being its junior synonym. Lectotype of Pentatoma viridissima Signoret, 1861 is designated, and lectotype designation of Pseudolerida incerta (Schouteden, 1909) is commented. The following new or confirmed state records are provided: Cappaeini: Leridella kenyensis Jeannel, 1913 (Cameroon), Paralerida bolivari (Schouteden, 1904) (Republic of the Congo, Uganda), P. niokana Linnavuori, 1982 (Uganda), Tripanda (Tripanda) dispar Schouteden, 1964 (Cameroon, Ghana, Liberia), T. (T.) horacekorum Kment & Jindra, 2009 (Cameroon, Guinea), T. (T.) jurickorum Kment & Jindra, 2009 (Gabon, Ivory Coast), T. (T.) longiceps (Villiers, 1967) (Democratic Republic of the Congo, Gabon), T. (T.) signitenens (Distant, 1898) (Democratic Republic of the Congo, Mozambique), T. (Tenerva) decorata (Jensen-Haarup, 1937) (Kenya, Oman, Tanzania); Carpocorini: Andocides vittaticeps (Angola); Diploxyini: Acoloba lanceolata (Fabricius, 1803) (Angola, Central African Republic, Zambia, Zimbabwe); Eysarcorini: Pseudolerida bitalensis Schouteden, 1958 (Cameroon), Pseudolerida incerta (Equatorial Guinea: Bioko Island, Tanzania, Uganda); Myrocheini: Humria bimaculicollis Linnavuori, 1975 (Botswana, Democratic Republic of the Congo, Guinea, Kenya, Mozambique, Namibia, South Africa, Zambia, Zimbabwe); Strachiini: Afrania brachyptera (Schaum, 1853) (Botswana, Democratic Republic of the Congo; Tanzania: Tanganyika, Zanzibar; Uganda). Variability of Tripanda jurickorum, Pseudolerida incerta and Humria bimaculicollis is discussed.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chen, Yong; Liu, Liguo; Fu, Hua

Highlights: • We utilized mTRAQ-based quantification to study protein changes in Congo red-induced OMVs. • A total of 148 proteins were identified in S. flexneri-derived OMVs. • Twenty-eight and five proteins are significantly up- and down-regulated in the CR-induced OMV, respectively. • The result implied that a special sorting mechanism of particular proteins into OMVs may exist. • Key node proteins in the protein interaction network might be important for pathogenicity. - Abstract: The production of outer membrane vesicles (OMVs) is a common and regulated process of gram-negative bacteria. Nonetheless, the processes of Shigella flexneri OMV production still remain unclear.more » S. flexneri is the causative agent of endemic shigellosis in developing countries. The Congo red binding of strains is associated with increased infectivity of S. flexneri. Therefore, understanding the modulation pattern of OMV protein expression induced by Congo red will help to elucidate the bacterial pathogenesis. In the present study, we investigated the proteomic composition of OMVs and the change in OMV protein expression induced by Congo red using mTRAQ-based quantitative comparative proteomics. mTRAQ labelling increased the confidence in protein identification, and 148 total proteins were identified in S. flexneri-derived OMVs. These include a variety of important virulence factors, including Ipa proteins, TolC family, murein hydrolases, and members of the serine protease autotransporters of Enterobacteriaceae (SPATEs) family. Among the identified proteins, 28 and five proteins are significantly up- and down-regulated in the Congo red-induced OMV, respectively. Additionally, by comprehensive comparison with previous studies focused on DH5a-derived OMV, we identified some key node proteins in the protein–protein interaction network that may be involved in OMV biogenesis and are common to all gram-negative bacteria.« less

What do we learn about the impact of extreme hydrological events on tropical wetlands from the synergistic use of altimetry from Sentinel-3/SARAL-Altika and L-Band radiometry from SMOS/SMAP ?

NASA Astrophysics Data System (ADS)

Al Bitar, Ahmad; Parrens, Marie; Frappart, Frederic; Cauduro Dias de Paiva, Rodrigo; Papa, Fabrice; Kerr, Yann

2017-04-01

What do we learn about the impact of extreme hydrological events on tropical wetlands from the synergistic use of altimetry from Sentinel-3/SARAL-Altika and L-Band radiometry from SMOS/SMAP ? The question of the contribution of the tropical basins to the carbon and water cycle remains an open question in the science community. The tropical basins are highly impact by the wetlands dynamics but the also the link with extreme events like El-Nino are yet to be clarified. The main reason to this uncertainty is that the monitoring of inland water surfaces via remote sensing over tropical areas is a difficult task because of impact of vegetation and cloud cover. The most common solution is to use microwave remote sensing. In this study we combine the use of L-band microwave brightness temperatures and altimetric data from SARAL/ALTIKA and Sentinel-3 to derive water storage maps at relatively high (7days) temporal frequency. This study concerns the Amazon and Congo basin. The water fraction in inland are estimated by inversing a first order radiative model is used to derive surface water over land from the brightness temperature measured by ESA SMOS and SMAP mission at coarse resolution (25 km x 25 km) and 7-days frequency. The product is compared to the static land cover map such as ESA CCI and the International Geosphere-Biosphere Program (IGBP) and also dynamic maps from GIEMS and SWAPS products. Water storage is then obtained by combining the altimetric data from SARAL/ALTIKA and Sentinel-3 to the water surface fraction using an hypsometric approach. The water surfaces and water storage products are then compared to precipitation data from GPM TRMM datasets and river discharge data from field data. The amplitudes and time shifts of the signals is compared based on the sub-basin definition from Hydroshed database. The dataset is then divided into years of strong and weak El-Nino signal and the anomaly is between the two dataset is compared. The results show a strong influence of EL-Nino on the time shift of the different components showing that the hydrological regime of wetlands is highly impacted by these extreme events with a differentiated impact when compared to precipitation. Since the wetlands have particular impacts on the dynamics of the water and carbon cycle of the tropical basins, the results suggest that the current approach using future more accurate SWOT mission data can help better understand the physical processes in these basins.

Intrinsic controls on the range of volumes, morphologies, and dimensions of submarine lobes

NASA Astrophysics Data System (ADS)

Prélat, A.; Covault, J. A.; Hodgson, D. M.; Fildani, A.; Flint, S. S.

2010-12-01

Submarine lobe dimensions from six different systems are compared: 1) the exhumed Permian Fan 3 lobe complex of the Tanqua Karoo, South Africa; 2) the modern Amazon fan channel-mouth lobe complex, offshore Brazil; 3) a portion of the modern distal Zaïre fan, offshore Angola/Congo; 4) a Pleistocene fan of the Kutai basin, subsurface offshore Indonesia; 5) the modern Golo system, offshore east Corsica, France; and 6) a shallow subsurface lobe complex , offshore Nigeria. These six systems have significantly different source-to-sink configurations (shelf dimension and slope topography), sediment supply characteristics (available grain size range and supply rate), tectonic settings, (palaeo) latitude, and delivery systems. Despite these differences, lobe deposits share similar geometric and dimensional characteristics. Lobes are grouped into two distinct populations of geometries that can be related to basin floor topography. The first population corresponds to areally extensive but thin lobes (average width 14 km × length 35 km × thickness 12 m) that were deposited onto low relief basin floor areas. Examples of such systems include the Tanqua Karoo, the Amazon, and the Zaïre systems. The second population corresponds to areally smaller but thicker lobes (average width 5 km × length 8 km × thickness 30 m) that were deposited into settings with higher amplitude of relief, like in the Corsican trough, the Kutai basin, and offshore Nigeria. The two populations of lobe types, however, share similar volumes (a narrow range around 1 or 2 km 3), which suggests that there is a control to the total volume of sediment that individual lobes can reach before they shift to a new locus of deposition. This indicates that the extrinsic processes control the number of lobes deposited per unit time rather than their dimensions. Two alternative hypotheses are presented to explain the similarities in lobe volumes calculated from the six very different systems. The first states that the wide range of starting flow volume and grain size across all systems enters the basin floor as a narrow range due to slope 'filtering' via more overspill and intra-channel deposition in larger systems. The second hypothesis is a result of the gradual decrease in downstream gradient from the distributive channel base to the lobe top during lobe growth. This is not sustainable as the channel will start to aggrade, and when a steeper lateral gradient is present, an avulsion will occur to an adjacent depositional low, which will be used for flows to fill and build a new lobe. This analysis of submarine lobe volumes indicates that the basin floor topography influences lobe geometry, but the fact that lobe volumes have a narrow range indicates a strong influence of intrinsic processes.

Intrinsic controls on the range of volumes, morphologies, and dimensions of submarine lobes

USGS Publications Warehouse

Prelat, A.; Covault, J.A.; Hodgson, D.M.; Fildani, A.; Flint, S.S.

2010-01-01

Submarine lobe dimensions from six different systems are compared: 1) the exhumed Permian Fan 3 lobe complex of the Tanqua Karoo, South Africa; 2) the modern Amazon fan channel-mouth lobe complex, offshore Brazil; 3) a portion of the modern distal Za??re fan, offshore Angola/Congo; 4) a Pleistocene fan of the Kutai basin, subsurface offshore Indonesia; 5) the modern Golo system, offshore east Corsica, France; and 6) a shallow subsurface lobe complex , offshore Nigeria. These six systems have significantly different source-to-sink configurations (shelf dimension and slope topography), sediment supply characteristics (available grain size range and supply rate), tectonic settings, (palaeo) latitude, and delivery systems. Despite these differences, lobe deposits share similar geometric and dimensional characteristics. Lobes are grouped into two distinct populations of geometries that can be related to basin floor topography. The first population corresponds to areally extensive but thin lobes (average width 14km??length 35km??thickness 12m) that were deposited onto low relief basin floor areas. Examples of such systems include the Tanqua Karoo, the Amazon, and the Za??re systems. The second population corresponds to areally smaller but thicker lobes (average width 5km??length 8km??thickness 30m) that were deposited into settings with higher amplitude of relief, like in the Corsican trough, the Kutai basin, and offshore Nigeria. The two populations of lobe types, however, share similar volumes (a narrow range around 1 or 2km3), which suggests that there is a control to the total volume of sediment that individual lobes can reach before they shift to a new locus of deposition. This indicates that the extrinsic processes control the number of lobes deposited per unit time rather than their dimensions. Two alternative hypotheses are presented to explain the similarities in lobe volumes calculated from the six very different systems. The first states that the wide range of starting flow volume and grain size across all systems enters the basin floor as a narrow range due to slope 'filtering' via more overspill and intra-channel deposition in larger systems. The second hypothesis is a result of the gradual decrease in downstream gradient from the distributive channel base to the lobe top during lobe growth. This is not sustainable as the channel will start to aggrade, and when a steeper lateral gradient is present, an avulsion will occur to an adjacent depositional low, which will be used for flows to fill and build a new lobe. This analysis of submarine lobe volumes indicates that the basin floor topography influences lobe geometry, but the fact that lobe volumes have a narrow range indicates a strong influence of intrinsic processes. ?? 2010 Elsevier B.V.

Source potential of the Zairian onshore pre-salt subbasins of the West African Aptian salt basin

DOE Office of Scientific and Technical Information (OSTI.GOV)

Swirydczuk, K.; Tshiband, D.; Nyimi, M.

1996-08-01

Three pre-salt subbasins are located onshore in Zaire in the Congo-Cabinda Basin. Production exists to the west, and extensive outcrops of Mavuma tar sands are located immediately to the east of these subbasins. Five pre-salt wells confirmed that thick Barremian lacustrine claystones of the Bucomazi Formation form the main source horizon in all the subbasins. Upper Bucomazi claystones average 4% and reach 12% TOC. Lower Bucomazi claystones average 2% (high of 6%). A mixed Type I/Type II algal oil-prone kerogen predominates. Up to 1% TOC is present in claystones in the underlying Lucula section. Dry pyrolysis shows significant differences inmore » kerogen kinetics from subbasin to subbasin. R{sub o} and T{sub max} were used to model heat flow through time. Ages were from biostratigraphic analyses and radiometric dating of thin volcanics within the Lucula and Bucomazi formations. Apatite fission track analyses provided control on uplift history. Pseudowells were used in maturation modelling to predict source rock maturity in the subbasins. The upper Bucomazi is immature except in the deeper parts of two of the subbasins. The Lower - Bucomazi and Upper Lucula are mature in all subbasins and in the deepest subbasins are overmature. Oil generation occurred shortly after deposition of the Loeme Salt. Analyses of Lindu oil support this early migration. Estimates of oil that may have been generated in the eastern-most subbasin suggest that extensive Mavuma tar sands, which have been typed to lacustrine source, could have been sourced from this subbasin.« less

Removal of Congo Red from Aqueous Solution by Anion Exchange Membrane (EBTAC): Adsorption Kinetics and Themodynamics.

PubMed

Khan, Muhammad Imran; Akhtar, Shahbaz; Zafar, Shagufta; Shaheen, Aqeela; Khan, Muhammad Ali; Luque, Rafael; Rehman, Aziz Ur

2015-07-08

The adsorption behavior of anionic dye congo red (CR) from aqueous solutions using an anion exchange membrane (EBTAC) has been investigated at room temperature. The effect of several factors including contact time, membrane dosage, ionic strength and temperature were studied. Kinetic models, namely pseudo-first-order and pseudo-second-order, liquid film diffusion and Elovich models as well as Bangham and modified freundlich Equations, were employed to evaluate the experimental results. Parameters such as adsorption capacities, rate constant and related correlation coefficients for every model were calculated and discussed. The adsorption of CR on anion exchange membranes followed pseudo-second-order Kinetics. Thermodynamic parameters, namely changes in Gibbs free energy ( ∆G° ), enthalpy ( ∆H° ) and entropy ( ∆S° ) were calculated for the adsorption of congo red, indicating an exothermic process.

Removal of Congo Red from Aqueous Solution by Anion Exchange Membrane (EBTAC): Adsorption Kinetics and Themodynamics

PubMed Central

Khan, Muhammad Imran; Akhtar, Shahbaz; Zafar, Shagufta; Shaheen, Aqeela; Khan, Muhammad Ali; Luque, Rafael; ur Rehman, Aziz

2015-01-01

The adsorption behavior of anionic dye congo red (CR) from aqueous solutions using an anion exchange membrane (EBTAC) has been investigated at room temperature. The effect of several factors including contact time, membrane dosage, ionic strength and temperature were studied. Kinetic models, namely pseudo-first-order and pseudo-second-order, liquid film diffusion and Elovich models as well as Bangham and modified freundlich Equations, were employed to evaluate the experimental results. Parameters such as adsorption capacities, rate constant and related correlation coefficients for every model were calculated and discussed. The adsorption of CR on anion exchange membranes followed pseudo-second-order Kinetics. Thermodynamic parameters, namely changes in Gibbs free energy (∆G°), enthalpy (∆H°) and entropy (∆S°) were calculated for the adsorption of congo red, indicating an exothermic process. PMID:28793430

Les granitoïdes de la couverture protérozoïque de la bordure nord du craton du Congo (Sud-Est du Cameroun et Sud-Ouest de la République centrafricaine), témoins d'une activité magmatique post-kibarienne à pré-panafricaineGranitoids of the Proterozoic cover of the Congo craton northern edge (South-East of Cameroon and South-West of the Central African Republic), witnesses of a post-Kibarian to pre-Pan-African magmatic activity

NASA Astrophysics Data System (ADS)

Vicat, Jean-Paul; Moloto-A-Kenguemba, Gaétan; Pouclet, André

2001-02-01

Granitoid bodies dated from the Late Mesoproterozoic intrude the Palaeoproterozoic cover of the northern edge of the Congo craton. They line up a north-south left-lateral shear zone related to the Late Kibaran tectonics. They originated from crustal melting, may be due to the thermal anomalies, that were responsible of the large basaltic production during the pre-Pan-African extension of the Central Africa rift system.

Genomic analysis of filoviruses associated with four viral hemorrhagic fever outbreaks in Uganda and the Democratic Republic of the Congo in 2012.

PubMed

Albariño, C G; Shoemaker, T; Khristova, M L; Wamala, J F; Muyembe, J J; Balinandi, S; Tumusiime, A; Campbell, S; Cannon, D; Gibbons, A; Bergeron, E; Bird, B; Dodd, K; Spiropoulou, C; Erickson, B R; Guerrero, L; Knust, B; Nichol, S T; Rollin, P E; Ströher, U

2013-08-01

In 2012, an unprecedented number of four distinct, partially overlapping filovirus-associated viral hemorrhagic fever outbreaks were detected in equatorial Africa. Analysis of complete virus genome sequences confirmed the reemergence of Sudan virus and Marburg virus in Uganda, and the first emergence of Bundibugyo virus in the Democratic Republic of the Congo. Published by Elsevier Inc.

Violence against sex workers by police and military in Democratic Republic of Congo.

PubMed

Ombeni, Alphonse Mihigo; Crago, Anna Louise

2008-12-01

Sex workers in the Sud-Kivu district of the Democratic Republic of Congo (DRC) are regularly subjected to sexual and other forms of violence. In this article, based on a presentation at a concurrent session at the conference, Alphonse Mihigo Ombeni and Anna Louise Crago describe the negative impacts of this violence on the sex workers' health and working conditions. Many have become HIV-positive.

Crimean Congo Hemorrhagic Fever Virus and Alkhurma (Alkhumra) Virus in Ticks in Djibouti.

PubMed

Horton, Katherine C; Fahmy, Nermeen T; Watany, Noha; Zayed, Alia; Mohamed, Abro; Ahmed, Ammar Abdo; Rollin, Pierre E; Dueger, Erica L

2016-10-01

Crimean Congo hemorrhagic fever virus and Alkhumra virus, not previously reported in Djibouti, were detected among 141 (infection rate = 15.7 per 100, 95% CI: 13.4-18.1) tick pools from 81 (37%) cattle and 2 (infection rate = 0.2 per 100, 95% CI: 0.0-0.7) tick pools from 2 (1%) cattle, respectively, collected at an abattoir in 2010 and 2011.

Malaria surveillance in the Democratic Republic of the Congo: comparison of microscopy, PCR, and rapid diagnostic test.

PubMed

Doctor, Stephanie M; Liu, Yunhao; Whitesell, Amy; Thwai, Kyaw L; Taylor, Steve M; Janko, Mark; Emch, Michael; Kashamuka, Melchior; Muwonga, Jérémie; Tshefu, Antoinette; Meshnick, Steven R

2016-05-01

Malaria surveillance is critical for control efforts, but diagnostic methods frequently disagree. Here, we compare microscopy, PCR, and a rapid diagnostic test in 7137 samples from children in the Democratic Republic of the Congo using latent class analysis. PCR had the highest sensitivity (94.6%) and microscopy had the lowest (76.7%). Copyright © 2016 Elsevier Inc. All rights reserved.

Group Trauma-Focused Cognitive-Behavioural Therapy with Former Child Soldiers and Other War-Affected Boys in the DR Congo: A Randomised Controlled Trial

ERIC Educational Resources Information Center

McMullen, John; O'Callaghan, Paul; Shannon, Ciaran; Black, Alastair; Eakin, John

2013-01-01

Background: The Democratic Republic of Congo (DRC) has been home to the world's deadliest conflict since World War II and is reported to have the largest number of child soldiers in the world. Despite evidence of the debilitating impact of war, no group-based mental health or psychosocial intervention has been evaluated in a randomised controlled…

[Preliminary results about loiasis in the district of Likouala in the Republic of the Congo].

PubMed

Akiana, J; Bokilo Dzia Lepfoundzou, A; Mokondjimobe, E; Parra, H J; Sokhna, C S; Faye, O

2013-05-01

The authors report results of a prospective study to assess the prevalence of loiasis in populations older than 18 years in two villages of the Likouala district in the Congo: the study took place during a visit to Lopola in 2012 and to Lopola and Lombo in 2013. The prevalence rate was 20%. Loa loa carriers were treated with diethylcarbamazine citrate and celestamine.

Relative Abundance and Diversity of Bacterial Methanotrophs at the Oxic–Anoxic Interface of the Congo Deep-Sea Fan

PubMed Central

Bessette, Sandrine; Moalic, Yann; Gautey, Sébastien; Lesongeur, Françoise; Godfroy, Anne; Toffin, Laurent

2017-01-01

Sitting at ∼5,000 m water depth on the Congo-Angola margin and ∼760 km offshore of the West African coast, the recent lobe complex of the Congo deep-sea fan receives large amounts of fluvial sediments (3–5% organic carbon). This organic-rich sedimentation area harbors habitats with chemosynthetic communities similar to those of cold seeps. In this study, we investigated relative abundance, diversity and distribution of aerobic methane-oxidizing bacteria (MOB) communities at the oxic–anoxic interface of sedimentary habitats by using fluorescence in situ hybridization and comparative sequence analysis of particulate mono-oxygenase (pmoA) genes. Our findings revealed that sedimentary habitats of the recent lobe complex hosted type I and type II MOB cells and comparisons of pmoA community compositions showed variations among the different organic-rich habitats. Furthermore, the pmoA lineages were taxonomically more diverse compared to methane seep environments and were related to those found at cold seeps. Surprisingly, MOB phylogenetic lineages typical of terrestrial environments were observed at such water depth. In contrast, MOB cells or pmoA sequences were not detected at the previous lobe complex that is disconnected from the Congo River inputs. PMID:28487684

Discrepancies between descriptions and illustrations of colours in Congo red-stained amyloid, and explanation of discrepant colours.

PubMed

Howie, Alexander J; Owen-Casey, Mared P

2010-09-01

Congo red-stained sections of amyloid may show various colours between crossed polariser and analyser. The aims were to see how papers described the colours, to compare descriptions with illustrations, and to explain the colours. In 160 papers on Congo red-stained amyloid, the commonest descriptions were 'green birefringence' and 'apple-green birefringence'. In 191 figures in 82 papers, 59 (31%) showed a pure green colour, 62 (32%) showed green and yellow or blue and yellow, 38 (20%) showed green and a colour other than yellow, mostly red, and 32 (17%) showed other colours. Discrepancies between colours reported and illustrated were noted in 127 figures (66%). Most (77) were between green alone in descriptions and green and another colour in figures, and 30 were between green in descriptions and no green at all in figures. Pure green can be seen in ideal conditions, but more often there are green and yellow, explained by strain birefringence, and green and red or other combinations, explained by uncrossing of polariser and analyser. These other anomalous colours are just as characteristic of amyloid as the pure green colour. Many papers on Congo red-stained amyloid appear to describe what is expected theoretically rather than what is actually seen.

Tumor size and stage of breast cancer in Côte d'Ivoire and Republic of Congo - Results from population-based cancer registries.

PubMed

Islami, Farhad; Lortet-Tieulent, Joannie; Okello, Catherine; Adoubi, Innocent; Mbalawa, Charles Gombé; Ward, Elizabeth M; Parkin, D Maxwell; Jemal, Ahmedin

2015-12-01

Breast cancer is now the leading female cancer in sub-Saharan Africa, but there is relatively little information on breast cancer characteristics from this region. We studied, on a population basis, the size and stage of female breast cancer at diagnosis in Côte d'Ivoire and Republic of Congo. Data on tumor size and stage of breast cancer at diagnosis were collected by population-based cancer registries in Abidjan (the capital of Côte d'Ivoire; 141 cases) and Brazzaville (the capital of Republic of Congo; 139 cases) from a random group of female breast cancer cases that were diagnosed in 2008-2009 using the same protocol. The majority of breast cancers in both countries were advanced cancers. In Côte d'Ivoire, 68% of tumors were ≥5 cm in diameter and 74% of cancers were stage III or IV at diagnosis; the corresponding proportions in Republic of Congo were 63% and 81%. These results underscore the importance of increased awareness about early detection of breast cancer, as well as expansion of the capacity to provide appropriate diagnosis, treatment, and palliative care in sub-Saharan Africa. Copyright © 2015 Elsevier Ltd. All rights reserved.

Investigation of Crimean-Congo Hemorrhagic Fever and Hemorrhagic Fever with Renal Syndrome in Greece

DTIC Science & Technology

1988-10-12

patients with rheumatoid arthritis (20 samples), with macroglobulinemia (3 samples), with pyrexia of unknown origin (41 samples) and from healthy...precomatose or comatose and 10 had renal failure with oliguria or anuria, requiring hemodialysis . The indication for dialysis in these patients was serum...against Hantaan vi- us and Crimean-Congo Hemorrhagic Fever respectively. Two hundred and two blood samples were aken from patients with disease resembling

The Democratic Republic of Congo: Background and Current Developments

DTIC Science & Technology

2011-04-29

Rwanda and Uganda, marched into Kinshasa and ousted longtime dictator Mobutu Sese Seko. Within one year, tensions between then- President Laurent...Congolese were registered to vote in the 2006 elections, including 2.9 million in Kinshasa , the capital. In the presidential elections, there were 33...Bemba and the Movement for the Liberation of Congo (MLC) In March 2007, the Congolese army and Bemba’s militia clashed in Kinshasa , forcing Bemba to

Intellectual and developmental disabilities in Kinshasa, Democratic Republic of the Congo: causality and implications for resilience and support.

PubMed

Aldersey, Heather M; Turnbull, H Rutherford; Turnbull, Ann P

2014-06-01

This article reports results of a 7-month qualitative study on intellectual and related developmental disabilities in Kinshasa, Democratic Republic of the Congo, particularly as they relate to the causes and meaning of intellectual and developmental disabilities (IDD). This study raises important questions related to the understanding of resilience of persons affected by IDD and the nature and purpose of support they use or desire.