Science.gov

Sample records for construction programs c-1

  1. 26 CFR 1.267(c)-1 - Constructive ownership of stock.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 3 2010-04-01 2010-04-01 false Constructive ownership of stock. 1.267(c)-1...) INCOME TAX (CONTINUED) INCOME TAXES Items Not Deductible § 1.267(c)-1 Constructive ownership of stock. (a) In general. (1) The determination of stock ownership for purposes of section 267(b) shall be...

  2. Computer Programs for Construction

    NASA Technical Reports Server (NTRS)

    1978-01-01

    A NASA computer program aids Hudson Engineering Corporation, Houston, Texas in the design and construction of huge petrochemical processing plants like the one shown, which is located at Ju'aymah, Saud Arabia. The pipes handling the flow of chemicals are subject to a variety of stresses, such as weight and variations in temperature. Hudson Engineering uses a COSMIC piping flexibility analysis computer program to analyze and insure the necessary strength and flexibility of the pipes. This program helps the company realize substantial savings in reduced engineering time.

  3. Ignitor Prototype Construction Program

    NASA Astrophysics Data System (ADS)

    Galas-So, G.; Lanzavecchia, L.; Dalmut, G.; Dra-Go, G.; Laurenti, A.; Marabotto, R.; Ghia, G.; Munaro, G.; Pirozzi, M.; Destefanis, L.; Andreani, R.; Crescenzi, C.; Cucchiaro, A.; Gasparotto, M.; Pizzuto, A.; Coppi, B.

    1997-11-01

    The design solutions adopted for Ignitor have been validated by a comprehensive engineering R&D program. The prototype construction program has been conducted by the industrial Consortium CITIF (ANSALDO and FIAT, with ABB as the main subcontractor). The manufacturing of full size prototypes of the most important components of the machine as well as tests on the adopted materials and on critical component interfaces have been carried out and have provided all the information necessary to continue the construction of the machine with the necessary confidence. In particular, an extensive test program has been launched to identify the mechanical properties of the ETP cold rolled copper plates produced for the toroidal field magnet. Tests under combined compressive/shear loads to evaluate the copper-insulation bonding shear strength have given positive results.

  4. Constructing Programs from Example Computations.

    ERIC Educational Resources Information Center

    Bierman, A. W.; Krishnaswamy, R.

    This paper describes the construction and implementation of an autoprogramming system. An autoprogrammer is an interactive computer programming system which automatically constructs computer programs from example computations executed by the user. The example calculations are done in a scratch pad fashion at a computer display, and the system…

  5. Construction of the programmed motion

    SciTech Connect

    Man, T.B.

    1982-08-01

    The objective of this paper is to outline some problems of the programmed motion. The Erugin's method for the construction of the set of differential equations according to the prescribed integrals, which plays a crucial role in the development of such motions, is discussed. Possible modifications of the inverse problem of dynamics is pointed out. One of the modifications, such as the construction of the functional, which takes stationary value on the solution of the constructed equations, has been worked out in detail.

  6. 40 CFR 265.19 - Construction quality assurance program.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... that the liners are constructed to meet the hydraulic conductivity requirements of §§ 264.221(c)(1... conductivity requirements must be verified by using in-situ testing on the constructed test fill. The test fill... conductivity requirements of §§ 264.221(c)(1), 264.251(c)(1), and 264.301(c)(1) of this chapter in the...

  7. Upgrading Programs for Construction Journeymen. Final Report.

    ERIC Educational Resources Information Center

    Franklin, William S.

    The report describes a study of industry-sponsored upgrading programs for journeymen in construction unions. Interviews with union and training officials, as well as 405 journeymen and 99 contractors, revealed that upgrading activities were concentrated in electrical work, carpentry, and the pipe trades, and that both the number of programs and…

  8. Complement Component C1q Programs a Pro-Efferocytic Phenotype while Limiting TNFα Production in Primary Mouse and Human Macrophages

    PubMed Central

    Hulsebus, Holly J.; O’Conner, Sean D.; Smith, Emily M.; Jie, Chunfa; Bohlson, Suzanne S.

    2016-01-01

    Deficiency in complement component C1q is associated with an inability to clear apoptotic cells (efferocytosis) and aberrant inflammation in lupus, and identification of the pathways involved in these processes should reveal important regulatory mechanisms in lupus and other autoimmune or inflammatory diseases. In this study, C1q-dependent regulation of TNFα/IL-6 expression and efferocytosis was investigated using primary mouse bone marrow-derived macrophages and human monocyte-derived macrophages. C1q downregulated LPS-dependent TNFα production in mouse and human macrophages. While prolonged stimulation with C1q (18 h) was required to elicit a dampening of TNFα production from mouse macrophages, the human macrophages responded to C1q with immediate downregulation of TNFα. IL-6 production was unchanged in mouse and upregulated by human macrophages following prolonged stimulation with C1q. Our previous studies indicated that C1q programmed enhanced efferocytosis in mouse macrophages by enhancing expression of Mer tyrosine kinase and its ligand Gas6, a receptor–ligand pair that also inhibits proinflammatory signaling. Here, we demonstrated that C1q-dependent programming of human macrophage efferocytosis required protein synthesis; however, neither Mer nor the related receptor Axl was upregulated in human cells. In addition, while the C1q-collagen-like tails are sufficient for promoting C1q-dependent phagocytosis of antibody-coated targets, the C1q-tails failed to program enhanced efferocytosis or dampen TNFα production. These data further elucidate the mechanisms by which C1q regulates proinflammatory signaling and efferocytosis in macrophages, functions that are likely to influence the progression of autoimmunity and chronic inflammation. PMID:27379094

  9. Epigenetic Regulation of Placental "NR3C1": Mechanism Underlying Prenatal Programming of Infant Neurobehavior by Maternal Smoking?

    ERIC Educational Resources Information Center

    Stroud, Laura R.; Papandonatos, George D.; Salisbury, Amy L.; Phipps, Maureen G.; Huestis, Marilyn A.; Niaura, Raymond; Padbury, James F.; Marsit, Carmen J.; Lester, Barry M.

    2016-01-01

    Epigenetic regulation of the placental glucocorticoid receptor gene ("NR3C1") was investigated as a mechanism underlying links between maternal smoking during pregnancy (MSDP) and infant neurobehavior in 45 mother-infant pairs (49% MSDP-exposed; 52% minorities; ages 18-35). The Neonatal Intensive Care Unit (NICU) Network Neurobehavioral…

  10. Flexible language constructs for large parallel programs

    NASA Technical Reports Server (NTRS)

    Rosing, Matthew; Schnabel, Robert

    1993-01-01

    The goal of the research described is to develop flexible language constructs for writing large data parallel numerical programs for distributed memory (MIMD) multiprocessors. Previously, several models have been developed to support synchronization and communication. Models for global synchronization include SIMD (Single Instruction Multiple Data), SPMD (Single Program Multiple Data), and sequential programs annotated with data distribution statements. The two primary models for communication include implicit communication based on shared memory and explicit communication based on messages. None of these models by themselves seem sufficient to permit the natural and efficient expression of the variety of algorithms that occur in large scientific computations. An overview of a new language that combines many of these programming models in a clean manner is given. This is done in a modular fashion such that different models can be combined to support large programs. Within a module, the selection of a model depends on the algorithm and its efficiency requirements. An overview of the language and discussion of some of the critical implementation details is given.

  11. Flexible Language Constructs for Large Parallel Programs

    DOE PAGESBeta

    Rosing, Matt; Schnabel, Robert

    1994-01-01

    The goal of the research described in this article is to develop flexible language constructs for writing large data parallel numerical programs for distributed memory (multiple instruction multiple data [MIMD]) multiprocessors. Previously, several models have been developed to support synchronization and communication. Models for global synchronization include single instruction multiple data (SIMD), single program multiple data (SPMD), and sequential programs annotated with data distribution statements. The two primary models for communication include implicit communication based on shared memory and explicit communication based on messages. None of these models by themselves seem sufficient to permit the natural and efficient expression ofmore » the variety of algorithms that occur in large scientific computations. In this article, we give an overview of a new language that combines many of these programming models in a clean manner. This is done in a modular fashion such that different models can be combined to support large programs. Within a module, the selection of a model depends on the algorithm and its efficiency requirements. In this article, we give an overview of the language and discuss some of the critical implementation details.« less

  12. Local School Construction Programs. Bulletin, 1957, No. 20

    ERIC Educational Resources Information Center

    Viles, N. E.

    1957-01-01

    This bulletin is designed as an outline to direct the attention of local officials to various features and steps meriting attention in planning and carrying out a school-plant construction program. Planning, administration, and construction practices and procedures may vary with buildings of different sizes or types of construction or with those…

  13. Construction safety program for the National Ignition Facility

    SciTech Connect

    Cerruti, S.J.

    1997-06-26

    The Construction Safety Program (CSP) for NIF sets forth the responsibilities, guidelines, rules, policies and regulations for all workers involved in the construction, special equipment installation, acceptance testing, and initial activation and operation of NIF at LLNL during the construction period of NIF.

  14. An Assessment of the Detroit Public Schools Construction Systems Program.

    ERIC Educational Resources Information Center

    Featherstone, Richard L.

    The Construction Systems Program (CSP) is the management process utilized in the construction of additions to four schools in the Detroit School District. The objectives of CSP were (1) to reduce the time required to plan and construct new buildings and additions, (2) to reduce the cost of new buildings and additions, and (3) to maintain or…

  15. Construction safety program for the National Ignition Facility

    SciTech Connect

    Cerruti, S.J.

    1997-01-01

    The Construction Safety Program (CSP) for NIF sets forth the responsibilities, guidelines, rules, policies and regulations for all workers involved in the construction, special equipment installation, acceptance testing, and initial activation and operation of NIF at LLNL during the construction period of NIF. During this period, all workers are required to implement measures to create a universal awareness which promotes safe practice at the work site, and which will achieve NIF`s management objectives in preventing accidents and illnesses. Construction safety for NIF is predicated on everyone performing their jobs in a manner which prevents job-related disabling injuries and illnesses. The CSP outlines the minimum environment, safety, and health (ES&H) standards, LLNL policies and the Construction Industry Institute (CII) Zero Injury Techniques requirements that all workers at the NIF construction site shall adhere to during the construction period of NIF. It identifies the safety requirements which the NIF organizational Elements, construction contractors and construction subcontractors must include in their safety plans for the construction period of NIF, and presents safety protocols and guidelines which workers shall follow to assure a safe and healthful work environment. The CSP also identifies the ES&H responsibilities of LLNL employees, non-LLNL employees, construction contractors, construction subcontractors, and various levels of management within the NIF Program at LLNL. In addition, the CSP contains the responsibilities and functions of ES&H support organizations and administrative groups, and describes their interactions with the NIF Program.

  16. 42 CFR 137.275 - May Self-Governance Tribes include IHS construction programs in a construction project agreement...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... programs in a construction project agreement or in a funding agreement? 137.275 Section 137.275 Public...-Governance Tribes include IHS construction programs in a construction project agreement or in a funding agreement? Yes, Self-Governance Tribes may choose to assume construction programs in a construction...

  17. 42 CFR 137.275 - May Self-Governance Tribes include IHS construction programs in a construction project agreement...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... programs in a construction project agreement or in a funding agreement? 137.275 Section 137.275 Public...-Governance Tribes include IHS construction programs in a construction project agreement or in a funding agreement? Yes, Self-Governance Tribes may choose to assume construction programs in a construction...

  18. 42 CFR 137.275 - May Self-Governance Tribes include IHS construction programs in a construction project agreement...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... programs in a construction project agreement or in a funding agreement? 137.275 Section 137.275 Public...-Governance Tribes include IHS construction programs in a construction project agreement or in a funding agreement? Yes, Self-Governance Tribes may choose to assume construction programs in a construction...

  19. Medical surveillance programs for construction workers.

    PubMed

    Welch, L; Roto, P

    1995-01-01

    In summary, the basic medical examination and carefully documented work and medical history are essential parts of the health examinations of construction workers. To achieve their best potential in preventing occupational and chronic diseases, the examinations should be conducted by professionals who know the working conditions in construction and have a positive attitude toward preventive medicine. One important aspect of these examinations is the opportunity to trigger workplace investigations, followed by exposure reduction. Simultaneously, such examinations provide an opportunity for health education of the worker and an education about work-related health problems for the health care provider.

  20. Youth Apprenticeship in Construction Trades. Student Handbook. Program Requirements.

    ERIC Educational Resources Information Center

    Manatee County Schools, Bradenton, FL.

    This student handbook contains information about participating in the construction trades program in the Manatee County (Florida) Public Schools. The first part of the handbook consists of general information about the program: program goals and objectives, intended outcomes, benefits to students, student responsibilities, contractor…

  1. Construction Pre-Apprenticeship Programs: Results from a National Survey

    ERIC Educational Resources Information Center

    Conway, Maureen; Gerber, Allison

    2009-01-01

    This publication shares findings from a WSI-conducted survey of pre-apprenticeship programs in the construction trades. Based on responses from 260 programs nationwide, the report presents information about program size, services offered, populations served, funding sources, and successes and challenges in placing trainees in apprenticeships or…

  2. 40 CFR 35.903 - Summary of construction grant program.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 1 2014-07-01 2014-07-01 false Summary of construction grant program. 35.903 Section 35.903 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE STATE AND LOCAL ASSISTANCE Grants for Construction of Treatment Works-Clean Water...

  3. 40 CFR 35.903 - Summary of construction grant program.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 1 2012-07-01 2012-07-01 false Summary of construction grant program. 35.903 Section 35.903 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE STATE AND LOCAL ASSISTANCE Grants for Construction of Treatment Works-Clean Water...

  4. 40 CFR 35.903 - Summary of construction grant program.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 1 2013-07-01 2013-07-01 false Summary of construction grant program. 35.903 Section 35.903 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE STATE AND LOCAL ASSISTANCE Grants for Construction of Treatment Works-Clean Water...

  5. Public School Construction Program. Administrative Procedures Guide. Revised.

    ERIC Educational Resources Information Center

    Maryland State Interagency Committee on School Construction, Baltimore.

    The State of Maryland has published this guide that provides State and local personnel, architects, and governmental officials with the method of operation and administration of the State's Public School Construction Program. The material is arranged in sequential order and follows a project from inception through design, construction, and…

  6. Development of a safety communication and recognition program for construction.

    PubMed

    Sparer, Emily H; Herrick, Robert F; Dennerlein, Jack T

    2015-05-01

    Leading-indicator-based (e.g., hazard recognition) incentive programs provide an alternative to controversial lagging-indicator-based (e.g., injury rates) programs. We designed a leading-indicator-based safety communication and recognition program that incentivized safe working conditions. The program was piloted for two months on a commercial construction worksite and then redesigned using qualitative interview and focus group data from management and workers. We then ran the redesigned program for six months on the same worksite. Foremen received detailed weekly feedback from safety inspections, and posters displayed worksite and subcontractor safety scores. In the final program design, the whole site, not individual subcontractors, was the unit of analysis and recognition. This received high levels of acceptance from workers, who noted increased levels of site unity and team-building. This pilot program showed that construction workers value solidarity with others on site, demonstrating the importance of health and safety programs that engage all workers through a reliable and consistent communication infrastructure.

  7. 40 CFR 264.19 - Construction quality assurance program.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... components, where applicable: (i) Foundations; (ii) Dikes; (iii) Low-permeability soil liners; (iv... 264.301. (2) The CQA program shall include test fills for compacted soil liners, using the same... fill, where data are sufficient to show that a constructed soil liner will meet the...

  8. Programmed Instruction: Construction Responding, Discrimination Responding, and Highlighted Keywords

    ERIC Educational Resources Information Center

    Miller, Matthew L.; Malott, Richard W.

    2006-01-01

    Skinner (1958) expressed the need for an increase in the effectiveness and efficiency of education. In particular, he suggested that programmed instruction could provide such efficiency. The present study used a within-subject design to compare the effects of four types of instructional materials; those requiring overt construction responses,…

  9. 40 CFR 264.19 - Construction quality assurance program.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... components, where applicable: (i) Foundations; (ii) Dikes; (iii) Low-permeability soil liners; (iv... 264.301. (2) The CQA program shall include test fills for compacted soil liners, using the same... fill, where data are sufficient to show that a constructed soil liner will meet the...

  10. 40 CFR 264.19 - Construction quality assurance program.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... components, where applicable: (i) Foundations; (ii) Dikes; (iii) Low-permeability soil liners; (iv... 264.301. (2) The CQA program shall include test fills for compacted soil liners, using the same... fill, where data are sufficient to show that a constructed soil liner will meet the...

  11. Language constructs and runtime systems for compositional parallel programming

    SciTech Connect

    Foster, I.; Kesselman, C.

    1995-03-01

    In task-parallel programs, diverse activities can take place concurrently, and communication and synchronization patterns are complex and not easily predictable. Previous work has identified compositionality as an important design principle for task-parallel programs. In this paper, we discuss alternative approaches to the realization of this principle. We first provide a review and critical analysis of Strand, an early compositional programming language. We examine the strengths of the Strand approach and also its weaknesses, which we attribute primarily to the use of a specialized language. Then, we present an alternative programming language framework that overcomes these weaknesses. This framework uses simple extensions to existing sequential languages (C++ and Fortran) and a common runtime system to provide a basis for the construction of large, task-parallel programs. We also discuss the runtime system techniques required to support these languages on parallel and distributed computer systems.

  12. 42 CFR 137.275 - May Self-Governance Tribes include IHS construction programs in a construction project agreement...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false May Self-Governance Tribes include IHS construction... OF HEALTH AND HUMAN SERVICES TRIBAL SELF-GOVERNANCE Construction Purpose and Scope § 137.275 May Self-Governance Tribes include IHS construction programs in a construction project agreement or in a...

  13. 42 CFR 137.275 - May Self-Governance Tribes include IHS construction programs in a construction project agreement...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 42 Public Health 1 2011-10-01 2011-10-01 false May Self-Governance Tribes include IHS construction... OF HEALTH AND HUMAN SERVICES TRIBAL SELF-GOVERNANCE Construction Purpose and Scope § 137.275 May Self-Governance Tribes include IHS construction programs in a construction project agreement or in a...

  14. 40 CFR 264.19 - Construction quality assurance program.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... conductivity requirements of §§ 264.221(c)(1)(i)(B), 264.251(c)(1)(i)(B), and 264.301(c)(1)(i)(B) in the field. Compliance with the hydraulic conductivity requirements must be verified by using in-situ testing on the... conductivity requirements of §§ 264.221(c)(1)(i)(B), 264.251(c)(1)(i)(B), and 264.301(c)(1)(i)(B) in the...

  15. Protein engineering studies on C1r and C1s.

    PubMed

    Závodszky, P; Gál, P; Cseh, S; Schumaker, V N

    1993-12-01

    1. C1r and C1s cDNAs were placed downstream the strong polyhedrin promoter in the Autographa californica nuclear polyhedrosis virus and the recombinant proteins were expressed in insect cells, in biologically active form. The yield of expression is high enough to get recombinant components for chemical and functional studies (5 micrograms/ml cell culture supernatant). 2. The biological activity and the post-translational modifications of the recombinant subcomponents were checked. The rC1r and rC1s proved to be biologically active in the hemolytic assay, although their glycosylations were different compared to that of the serum proteins. The insect cells are able to beta-hydroxylate the Asn residue of the EGF domain in the C1r but with a low efficiency. It is clear now, that this post-translational modification does not play a role in the Ca2+ dependent C1r-C1s interaction. 3. Two deletion mutants of C1r cDNA were constructed in order to clarify the role of domain I and II. The results show that both, domain I, and II are absolutely necessary for the tetramer formation and both have a regulatory role in the autoactivation. The autoactivation of the mutants is accelerated significantly. 4. Hybrid cDNA constructions were also made, and one of them was expressed. In the C1s alpha R hybrid the C1s alpha part cannot dimerize in presence of Ca2+, but it can form a tetramer with C1r2, that can bind to C1q. This observation indicates that the function of the C1s alpha part in the hybrid is modulated by the C1r part (gamma B) of the molecule. 5. In order to control the autoactivation process point mutant cDNAs were constructed through altering the Arg-Ile bond in the catalytic domain of the C1r. The Gln-Ile construction is a stable zymogen while the Arg-Phe mutant has a lower rate of autoactivation. These results do justify our approach of using domain-domain interchange, domain deletion and point mutations in combination, to reveal the structural background of C1 function

  16. 78 FR 13646 - Applications for New Awards; Impact Aid Discretionary Construction Grant Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-28

    ... Applications for New Awards; Impact Aid Discretionary Construction Grant Program AGENCY: Office of Elementary and Secondary Education, Department of Education. ACTION: Notice. Overview Information Impact Aid... Impact Aid Discretionary Construction Grant program provides grants for emergency repairs...

  17. 77 FR 28573 - Applications for New Awards; Impact Aid Discretionary Construction Grant Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-15

    ... Applications for New Awards; Impact Aid Discretionary Construction Grant Program AGENCY: Office of Elementary and Secondary Education, Department of Education ACTION: Notice. Overview Information Impact Aid... Aid Discretionary Construction Grant program provides grants for emergency repairs and...

  18. Feasibility of home infusion and self-administration of nanofiltered C1 esterase inhibitor for routine prophylaxis in patients with hereditary angioedema and characterization of a training and support program.

    PubMed

    Gregory, Cherylann; Landmesser, Ladonna M; Corrigan, Larry; Mariano, David

    2014-01-01

    Hereditary angioedema (HAE) is a rare, chronic disease of C1 inhibitor deficiency. Study researchers evaluated the prevalence of home and self-administration of nanofiltered, human-derived C1 esterase inhibitor infusions and the implementation of a nursing training and support program. Home administration rate increased from 49.0% to 75.8%. The percentage who self-administered increased from 20.3% to 43.9%. Doses per week averaged 1.85 at home compared with 1.40 in infusion centers and physicians' offices. Patients required an average of 5 visits to be trained. Self-administration is a viable, feasible option in the management of HAE, which is facilitated by a nurse-managed training and support program.

  19. 48 CFR 228.102-70 - Defense Environmental Restoration Program construction contracts.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Restoration Program construction contracts. 228.102-70 Section 228.102-70 Federal Acquisition Regulations... BONDS AND INSURANCE Bonds 228.102-70 Defense Environmental Restoration Program construction contracts. For Defense Environmental Restoration Program construction contracts entered into pursuant to 10...

  20. 48 CFR 228.102-70 - Defense Environmental Restoration Program construction contracts.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Restoration Program construction contracts. 228.102-70 Section 228.102-70 Federal Acquisition Regulations... BONDS AND INSURANCE Bonds 228.102-70 Defense Environmental Restoration Program construction contracts. For Defense Environmental Restoration Program construction contracts entered into pursuant to 10...

  1. Better Space Construction Decisions by Instructional Program Simulation Utilizing the Generalized Academic Simulation Programs.

    ERIC Educational Resources Information Center

    Apker, Wesley

    This school district utilized the generalized academic simulation programs (GASP) to assist in making decisions regarding the kinds of facilities that should be constructed at Pilchuck Senior High School. Modular scheduling was one of the basic educational parameters used in determining the number and type of facilities needed. The objectives of…

  2. Genetic programs constructed from layered logic gates in single cells

    PubMed Central

    Moon, Tae Seok; Lou, Chunbo; Tamsir, Alvin; Stanton, Brynne C.; Voigt, Christopher A.

    2014-01-01

    Genetic programs function to integrate environmental sensors, implement signal processing algorithms and control expression dynamics1. These programs consist of integrated genetic circuits that individually implement operations ranging from digital logic to dynamic circuits2–6, and they have been used in various cellular engineering applications, including the implementation of process control in metabolic networks and the coordination of spatial differentiation in artificial tissues. A key limitation is that the circuits are based on biochemical interactions occurring in the confined volume of the cell, so the size of programs has been limited to a few circuits1,7. Here we apply part mining and directed evolution to build a set of transcriptional AND gates in Escherichia coli. Each AND gate integrates two promoter inputs and controls one promoter output. This allows the gates to be layered by having the output promoter of an upstream circuit serve as the input promoter for a downstream circuit. Each gate consists of a transcription factor that requires a second chaperone protein to activate the output promoter. Multiple activator–chaperone pairs are identified from type III secretion pathways in different strains of bacteria. Directed evolution is applied to increase the dynamic range and orthogonality of the circuits. These gates are connected in different permutations to form programs, the largest of which is a 4-input AND gate that consists of 3 circuits that integrate 4 inducible systems, thus requiring 11 regulatory proteins. Measuring the performance of individual gates is sufficient to capture the behaviour of the complete program. Errors in the output due to delays (faults), a common problem for layered circuits, are not observed. This work demonstrates the successful layering of orthogonal logic gates, a design strategy that could enable the construction of large, integrated circuits in single cells. PMID:23041931

  3. 1-Nitropyrene (1-NP) induces apoptosis and apparently a non-apoptotic programmed cell death (paraptosis) in Hepa1c1c7 cells

    SciTech Connect

    Asare, Nana Landvik, Nina E.; Lagadic-Gossmann, Dominique; Rissel, Mary; Tekpli, Xavier; Ask, Kjetil; Lag, Marit; Holme, Jorn A.

    2008-07-15

    Mechanistic studies of nitro-PAHs (polycyclic aromatic hydrocarbons) of interest might help elucidate which chemical characteristics are most important in eliciting toxic effects. 1-Nitropyrene (1-NP) is the predominant nitrated PAH emitted in diesel exhaust. 1-NP-exposed Hepa1c1c7 cells exhibited marked changes in cellular morphology, decreased proliferation and different forms of cell death. A dramatic increase in cytoplasmic vacuolization was observed already after 6 h of exposure and the cells started to round up at 12 h. The rate of cell proliferation was markedly reduced at 24 h and apoptotic as well as propidium iodide (PI)-positive cells appeared. Electron microscopic examination revealed that the vacuolization was partly due to mitochondria swelling. The caspase inhibitor Z-VAD-FMK inhibited only the apoptotic cell death and Nec-1 (an inhibitor of necroptosis) exhibited no inhibitory effects on either cell death or vacuolization. In contrast, cycloheximide markedly reduced both the number of apoptotic and PI-positive cells as well as the cytoplasmic vacuolization, suggesting that 1-NP induced paraptotic cell death. All the MAPKs; ERK1/2, p38 and JNK, appear to be involved in the death process since marked activation was observed upon 1-NP exposure, and their inhibitors partly reduced the induced cell death. The ERK1/2 inhibitor PD 98057 completely blocked the induced vacuolization, whereas the other MAPKs inhibitors only had minor effects on this process. These findings suggest that 1-NP may cause apoptosis and paraptosis. In contrast, the corresponding amine (1-aminopyrene) elicited only minor apoptotic and necrotic cell death, and cells with characteristics typical of paraptosis were absent.

  4. 38 CFR 17.255 - Applications for grants for programs which include construction projects.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... for programs which include construction projects. 17.255 Section 17.255 Pensions, Bonuses, and... Applications for grants for programs which include construction projects. In addition to the documents and... specifications for the construction project, and where applicable, sufficient explanations of...

  5. 38 CFR 17.255 - Applications for grants for programs which include construction projects.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... for programs which include construction projects. 17.255 Section 17.255 Pensions, Bonuses, and... Applications for grants for programs which include construction projects. In addition to the documents and... specifications for the construction project, and where applicable, sufficient explanations of...

  6. 38 CFR 17.255 - Applications for grants for programs which include construction projects.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... for programs which include construction projects. 17.255 Section 17.255 Pensions, Bonuses, and... Applications for grants for programs which include construction projects. In addition to the documents and... specifications for the construction project, and where applicable, sufficient explanations of...

  7. 38 CFR 17.255 - Applications for grants for programs which include construction projects.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... for programs which include construction projects. 17.255 Section 17.255 Pensions, Bonuses, and... Applications for grants for programs which include construction projects. In addition to the documents and... specifications for the construction project, and where applicable, sufficient explanations of...

  8. 38 CFR 17.255 - Applications for grants for programs which include construction projects.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... for programs which include construction projects. 17.255 Section 17.255 Pensions, Bonuses, and... Applications for grants for programs which include construction projects. In addition to the documents and... specifications for the construction project, and where applicable, sufficient explanations of...

  9. 42 CFR 52c.1 - Applicability.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GRANTS MINORITY BIOMEDICAL RESEARCH SUPPORT PROGRAM § 52c.1 Applicability. The regulations in this part apply to grants (under the Minority... Service (PHS) Act (42 U.S.C. 241(a)(3)) to increase the numbers of ethnic minority faculty, students,...

  10. 42 CFR 52c.1 - Applicability.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GRANTS MINORITY BIOMEDICAL RESEARCH SUPPORT PROGRAM § 52c.1 Applicability. The regulations in this part apply to grants (under the Minority... Service (PHS) Act (42 U.S.C. 241(a)(3)) to increase the numbers of ethnic minority faculty, students,...

  11. 42 CFR 52c.1 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GRANTS MINORITY BIOMEDICAL RESEARCH SUPPORT PROGRAM § 52c.1 Applicability. The regulations in this part apply to grants (under the Minority... Service (PHS) Act (42 U.S.C. 241(a)(3)) to increase the numbers of ethnic minority faculty, students,...

  12. 42 CFR 52c.1 - Applicability.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GRANTS MINORITY BIOMEDICAL RESEARCH SUPPORT PROGRAM § 52c.1 Applicability. The regulations in this part apply to grants (under the Minority... Service (PHS) Act (42 U.S.C. 241(a)(3)) to increase the numbers of ethnic minority faculty, students,...

  13. 42 CFR 52c.1 - Applicability.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GRANTS MINORITY BIOMEDICAL RESEARCH SUPPORT PROGRAM § 52c.1 Applicability. The regulations in this part apply to grants (under the Minority... Service (PHS) Act (42 U.S.C. 241(a)(3)) to increase the numbers of ethnic minority faculty, students,...

  14. Corporate Mentors and Undergraduate Students: A Qualitative Study of the Advancing Women in Construction Mentorship Program

    ERIC Educational Resources Information Center

    Eicher, Matthew

    2013-01-01

    In a conscious effort to combat the low enrollment of women in construction management, a program was created to retain women through a mentorship program--Advancing Women in Construction. A qualitative analysis, facilitated through a grounded theory approach, sought to understand if the program was indeed successful, and what value did the…

  15. Extravehicular Crewman Work System (ECWS) study program. Volume 2: Construction

    NASA Technical Reports Server (NTRS)

    Wilde, R. C.

    1980-01-01

    The construction portion of the Extravehicular Crewman Work System Study defines the requirements and selects the concepts for the crewman work system required to support the construction of large structures in space.

  16. Constructing and Verifying Program Theory Using Source Documentation

    ERIC Educational Resources Information Center

    Renger, Ralph

    2010-01-01

    Making the program theory explicit is an essential first step in Theory Driven Evaluation (TDE). Once explicit, the program logic can be established making necessary links between the program theory, activities, and outcomes. Despite its importance evaluators often encounter situations where the program theory is not explicitly stated. Under such…

  17. Constructing a Conceptual Framework for Elementary Algebra through Logo Programming.

    ERIC Educational Resources Information Center

    Noss, Richard

    1986-01-01

    This study, part of a longitudinal investigation, examined the kinds of thinking which children aged 10 and 11 could carry over from Logo instruction to an algebraic context. Interviews focused on their ability to construct meaningful symbolization for the concept of variable and to construct formalized algebraic rules. (MNS)

  18. Guidelines for Selecting a Construction Approach for Education Building Programs.

    ERIC Educational Resources Information Center

    Barton Malow Co., Southfield, MI.

    This book discusses the advantages and disadvantages of the two most common construction planning methods utilized for educational facilities: general contracting and construction management. Diagrams are provided that illustrate the chain of command and communication within each approach, and highlight considerations that every school district…

  19. A Solid Foundation: Key Capacities of Construction Pre-Apprenticeship Programs

    ERIC Educational Resources Information Center

    Helmer, Matt; Blair, Amy; Gerber, Allison

    2012-01-01

    This publication shares research from site visits conducted to construction pre-apprenticeship programs in Baltimore, Hartford, Milwaukee and Portland (OR). Findings from the site visits, which included interviews and focus groups with pre-apprenticeship program staff, public officials, philanthropic leaders, construction industry leaders and…

  20. Adults' Perceptions of Knowledge Construction as Participants in Nonformal World Affairs Programs: An Interpretive Study

    ERIC Educational Resources Information Center

    Yelich Biniecki, Susan M.

    2015-01-01

    The purpose of this interpretive research study was to explore how adult learners perceive they construct knowledge in connection to their participation in nonformal world affairs programs. The study context involved the exploration of 12 adult learners' perceptions of their knowledge construction as participants in world affairs programs held in…

  1. 78 FR 25062 - Program for Construction, Renovation, Repair or Expansion of Public Schools Located on Military...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-29

    ... of the Secretary Program for Construction, Renovation, Repair or Expansion of Public Schools Located... program to construct, renovate, repair, or expand elementary and ] secondary public schools on military..., as described in the Federal Register notice dated September 9, 2011 (76 FR 55883-55886) and...

  2. 40 CFR 12.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 1 2012-07-01 2012-07-01 false Program accessibility: New construction and alterations. 12.151 Section 12.151 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... ENVIRONMENTAL PROTECTION AGENCY § 12.151 Program accessibility: New construction and alterations. Each...

  3. 40 CFR 12.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 1 2014-07-01 2014-07-01 false Program accessibility: New construction and alterations. 12.151 Section 12.151 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... ENVIRONMENTAL PROTECTION AGENCY § 12.151 Program accessibility: New construction and alterations. Each...

  4. New Tactics for Building: Experience/Analysis/Recommendations from the Detroit Public Schools Construction Systems Program.

    ERIC Educational Resources Information Center

    Cleland, Wallace B., Ed.

    This study is focused on organizational and procedural aspects of the Construction Systems Program (CSP), a developmental/demonstration project. The report is derived principally from practical observations by 42 active participants (architects, engineers, contractors, construction managers, owner's staff) in a four-school pilot program. Also…

  5. Labor productivity in heavy construction: impact on synfuels program employment

    SciTech Connect

    Kusterer, K.C.

    1980-06-01

    This study focuses on variations in labor productivity in the heavy construction industry. Productivity is one of a number of factors likely to affect the speed and cost of constructing a synthetic fuels plant. The findings of this study are presented with reference to synthetic fuels plants, but they are relevant to other large energy facilities as well. The data were gathered through a detailed literature search and extensive in-depth interviews with consultants in heavy industrial construction, union officials, and management. In this manner the most important determinants of labor productivity were identified and ranked in terms of relative significance. The type of project under construction is the most important factor affecting the productivity of heavy construction labor. Projects characterized by the utility work syndrome are large, complex, relatively unique, highly regulated, and have cost-plus contracts and tight deadlines. Such projects generally have lower-than-average levels of labor productivity. Labor productivity is also lowered by worker and management morale problems, due to delays and design changes, and by high levels of unemployment among construction workers. Finally, boom town conditions, caused by workers moving to live near large projects located in rural areas, also are likely to result in below-average labor productivity.Synthetic fuels plants are likely to have a number of these identified characteristics. Consequently, the findings suggest that labor productivity may well be a problem for the timely development of an economically competitive synthetic fuels industry.

  6. C1-inhibitor and transplantation.

    PubMed

    Kirschfink, Michael

    2002-09-01

    Excessive activation of the protein cascade systems has been associated with post-transplantation inflammatory disorders. There is increasing evidence that complement not only significantly contributes to ischemia/reperfusion injury upon cold storage of the organ but also, although to a different degree, to allograft rejection. Complement activation is most fulminant in hyperacute rejection but seems also to contribute to acute transplant rejection. Therapeutic substitution of appropriate regulators, therefore, appears to be a reasonable approach to reduce undesirable inflammatory reactions in the grafted organ. C1-inhibitor, a multifunctional regulator of the various kinin-generating cascade systems (for review see: E. Hack, chapter in this issue), is frequently reduced in patients suffering from severe inflammatory disorders. Studies applying pathophysiologically relevant animal models of allo- and xenotransplantation as well as promising first clinical results from successful allotransplantation now provide evidence that C1-inhibitor may also serve as an effective means to protect the grafted organ against inflammatory tissue injury. In xenotransplantation, complement inhibition by specific regulators such as C1-inhibitor may help to overcome hyperacute graft rejection. After a brief introduction on the significance of complement to allo- and xenotransplantation the following review will focus on the impact of C1-inhibitor treatment on transplantation-associated inflammatory disorders, where complement contributes to the pathogenesis.

  7. An Approach to Effortless Construction of Program Animations

    ERIC Educational Resources Information Center

    Velazquez-Iturbide, J. Angel; Pareja-Flores, Cristobal; Urquiza-Fuentes, Jaime

    2008-01-01

    Program animation systems have not been as widely adopted by computer science educators as we might expect from the firm belief that they can help in enhancing computer science education. One of the most notable obstacles to their adoption is the considerable effort that the production of program animations represents for the instructor. We…

  8. 40 CFR 265.19 - Construction quality assurance program.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... of inspection and sampling activities for all unit components identified in paragraph (a)(2) of this.... (c) Contents of program. (1) The CQA program must include observations, inspections, tests, and... specifications and good engineering practices, and proper installation of all components (e.g., pipes)...

  9. Utility residential new construction programs: Going beyond the code. A report from the Database on Energy Efficiency Programs (DEEP) Project

    SciTech Connect

    Vine, E.

    1995-08-01

    Based on an evaluation of 10 residential new construction programs, primarily sponsored by investor-owned utilities in the United States, we find that many of these programs are in dire straits and are in danger of being discontinued because current inclusion of only direct program effects leads to the conclusion that they are not cost-effective. We believe that the cost-effectiveness of residential new construction programs can be improved by: (1) promoting technologies and advanced building design practices that significantly exceed state and federal standards; (2) reducing program marketing costs and developing more effective marketing strategies; (3) recognizing the role of these programs in increasing compliance with existing state building codes; and (4) allowing utilities to obtain an ``energy-savings credit`` from utility regulators for program spillover (market transformation) impacts. Utilities can also leverage their resources in seizing these opportunities by forming strong and trusting partnerships with the building community and with local and state government.

  10. Financial Awareness Education with Apprentices in the Australian Construction Industry: Program Evaluation

    ERIC Educational Resources Information Center

    Du Plessis, Karin; Green, Emma

    2013-01-01

    A financial awareness education program was implemented with construction industry apprentices in Victoria, Australia. The program included face-to-face delivery of education around a range of financial management issues that apprentices face as they begin their apprenticeship. The paper reports on an evaluation of the program, which included…

  11. 40 CFR 265.19 - Construction quality assurance program.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... components, where applicable: (i) Foundations; (ii) Dikes; (iii) Low-permeability soil liners; (iv... fills for compacted soil liners, using the same compaction methods as in the full-scale unit, to ensure... requirement is waived where data are sufficient to show that a constructed soil liner meets the...

  12. 40 CFR 265.19 - Construction quality assurance program.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... components, where applicable: (i) Foundations; (ii) Dikes; (iii) Low-permeability soil liners; (iv... fills for compacted soil liners, using the same compaction methods as in the full-scale unit, to ensure... requirement is waived where data are sufficient to show that a constructed soil liner meets the...

  13. 40 CFR 265.19 - Construction quality assurance program.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... components, where applicable: (i) Foundations; (ii) Dikes; (iii) Low-permeability soil liners; (iv... fills for compacted soil liners, using the same compaction methods as in the full-scale unit, to ensure... requirement is waived where data are sufficient to show that a constructed soil liner meets the...

  14. Algorithm for constructing the programmed motion of a bounding vehicle for the flight phase

    NASA Technical Reports Server (NTRS)

    Lapshin, V. V.

    1979-01-01

    The construction of the programmed motion of a multileg bounding vehicle in the flight was studied. An algorithm is given for solving the boundary value problem for constructing this programmed motion. If the motion is shown to be symmetrical, a simplified use of the algorithm can be applied. A method is proposed for nonimpact of the legs during lift-off of the vehicle, and for softness at touchdown. Tables are utilized to construct this programmed motion over a broad set of standard motion conditions.

  15. COOPERATIVE RESEARCH IN C1 CHEMISTRY

    SciTech Connect

    Gerald P. Huffman

    2001-04-30

    Faculty and students from five universities (Kentucky, West Virginia, Utah, Pittsburgh and Auburn) are collaborating on a basic research program to develop novel C1 chemistry processes for the production of clean, high quality transportation fuel. An Industrial Advisory Board (IAB) with members from Chevron, Eastman Chemical, Energy International, Teir Associates, and the Department of Defense has been formed to provide practical guidance to the program. The program has two principal objectives. (1) Develop technology for conversion of C1 source materials (natural gas, synthesis gas, carbon dioxide and monoxide, and methanol) into clean, high efficiency transportation fuel. (2) Develop novel processes for producing hydrogen from natural gas and other hydrocarbons. Some of the principal accomplishments of the program in its first two years are: (1) The addition of acetylenic compounds in Fischer-Tropsch synthesis is found to produce significant amounts of oxygenated products in FT diesel fuels. Such oxygenated products should decrease particulate matter (PM) emissions. (2) Nanoscale, binary, Fe-based catalysts supported on alumina have been shown to have significant activity for the decomposition of methane into pure hydrogen and potentially valuable multi-walled carbon nanotubes. (3) Catalytic synthesis processes have been developed for synthesis of diethyl carbonate, higher ethers, and higher alcohols from C1 source materials. Testing of the effect of adding these oxygenates to diesel fuel on PM emissions has begun using a well-equipped small diesel engine test facility. (4) Supercritical fluid (SCF) FT synthesis has been conducted under SCF hexane using both Fe and Co catalysts. There is a marked effect on the hydrocarbon product distribution, with a shift to higher carbon number products. These and other results are summarized.

  16. Continuing medical education, needs assessment, and program development: theoretical constructs.

    PubMed

    Aherne, M; Lamble, W; Davis, P

    2001-01-01

    Continuing medical education (CME) program development and needs assessment have historically been practiced within the tradition of Ralph Tyler's education model. In light of transformational social, political, economic, and technical forces that demand greater account-ability and responsiveness from physicians, CME units are challenged to transform their cultures and structures from models that deliver education to models that support the facilitation of learning for enhanced competence and performance. This article describes key change forces for physicians and brings program development and needs assessment into focus for the discussion. The impact of change forces on program development and needs assessment are examined, and some techniques to move beyond the traditional approach of felt needs are presented as a way of enabling strategic administrative planning and change management. PMID:11291588

  17. Construction safety program for the National Ignition Facility, Appendix B

    SciTech Connect

    Cerruti, S.J.

    1997-06-26

    This Appendix contains material from the LLNL Health and Safety Manual as listed below. For sections not included in this list, please refer to the Manual itself. The areas covered are: asbestos, lead, fire prevention, lockout, and tag program confined space traffic safety.

  18. Construction Management Program Builds Financial Development from the Ground up

    ERIC Educational Resources Information Center

    Nobe, Michael D.; Shuler, Scott; Grosse, Larry

    2007-01-01

    Recent economic and legislative changes have hit higher education hard and threaten the financial viability of many educational programs nationwide. With state support dwindling to less than 10 percent in some cases, institutions across the nation face a financial crisis. Many strategies have been explored and implemented, from campaigns to…

  19. 40 CFR 264.19 - Construction quality assurance program.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... implementation of the CQA plan, and CQA officer qualifications. (3) A description of inspection and sampling... CQA program must include observations, inspections, tests, and measurements sufficient to ensure: (i... practices, and proper installation of all components (e.g., pipes) according to design specifications;...

  20. Construction safety program for the National Ignition Facility, Appendix A

    SciTech Connect

    Cerruti, S.J.

    1997-06-26

    Topics covered in this appendix include: General Rules-Code of Safe Practices; 2. Personal Protective Equipment; Hazardous Material Control; Traffic Control; Fire Prevention; Sanitation and First Aid; Confined Space Safety Requirements; Ladders and Stairways; Scaffolding and Lift Safety; Machinery, Vehicles, and Heavy Equipment; Welding and Cutting-General; Arc Welding; Oxygen/Acetylene Welding and Cutting; Excavation, Trenching, and Shoring; Fall Protection; Steel Erection; Working With Asbestos; Radiation Safety; Hand Tools; Electrical Safety; Nonelectrical Work Performed Near Exposed High-Voltage Power-Distribution Equipment; Lockout/Tagout Requirements; Rigging; A-Cranes; Housekeeping; Material Handling and Storage; Lead; Concrete and Masonry Construction.

  1. 36 CFR 406.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to 101-19.607, apply to buildings... construction and alterations. 406.151 Section 406.151 Parks, Forests, and Public Property AMERICAN BATTLE... CONDUCTED BY AMERICAN BATTLE MONUMENTS COMMISSION § 406.151 Program accessibility: New construction...

  2. 36 CFR 406.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to 101-19.607, apply to buildings... construction and alterations. 406.151 Section 406.151 Parks, Forests, and Public Property AMERICAN BATTLE... CONDUCTED BY AMERICAN BATTLE MONUMENTS COMMISSION § 406.151 Program accessibility: New construction...

  3. 36 CFR 406.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to 101-19.607, apply to buildings... construction and alterations. 406.151 Section 406.151 Parks, Forests, and Public Property AMERICAN BATTLE... CONDUCTED BY AMERICAN BATTLE MONUMENTS COMMISSION § 406.151 Program accessibility: New construction...

  4. 36 CFR 406.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to 101-19.607, apply to buildings... construction and alterations. 406.151 Section 406.151 Parks, Forests, and Public Property AMERICAN BATTLE... CONDUCTED BY AMERICAN BATTLE MONUMENTS COMMISSION § 406.151 Program accessibility: New construction...

  5. RECOMMENDED FOUNDATION FILL MATERIALS CONSTRUCTION STANDARD OF THE FLORIDA RADON RESEARCH PROGRAM

    EPA Science Inventory

    The report summarizes the technical basis for a recommended foundation fill materials standard for new construction houses in Florida. he radon-control construction standard was developed by the Florida Radon Research Program (FRRP). ill material standards are formulated for: (1)...

  6. 76 FR 55883 - Department of Defense Program for Construction, Renovation, Repair or Expansion of Public Schools...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-09

    ... of the Secretary Department of Defense Program for Construction, Renovation, Repair or Expansion of... by OEA, to distribute $250 million made available by Congress to construct, renovate, repair, or..., repair, or expand elementary and secondary public schools on military installations in order to...

  7. 45 CFR 1153.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...) NATIONAL FOUNDATION ON THE ARTS AND THE HUMANITIES NATIONAL ENDOWMENT FOR THE ARTS ENFORCEMENT OF... ARTS § 1153.151 Program accessibility: New construction and alterations. Each building or part of a building that is constructed or altered by, on behalf of, or for the use of the agency shall be...

  8. A Program for Constructing and Scoring Several Types of Rating Scales and Checklists.

    ERIC Educational Resources Information Center

    Aiken, Lewis R.

    1996-01-01

    This article describes a set of 11 menu-driven procedures written in BASICA for MS-DOS based microcomputers for constructing several types of rating scales, attitude scales, and checklists, and for scoring responses to the constructed instruments. The uses of the program are described in detail. (SLD)

  9. Construction: Carpentry Concentration. Program CIP: 46.0000 Construction: Carpentry Concentration

    ERIC Educational Resources Information Center

    Agee, Kelly, Ed.

    2008-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  10. Cooperative Research in C1 Chemistry

    SciTech Connect

    Gerald P. Huffman

    2000-10-27

    C1 chemistry refers to the conversion of simple carbon-containing materials that contain one carbon atom per molecule into valuable products. The feedstocks for C1 chemistry include natural gas, carbon dioxide, carbon monoxide, methanol and synthesis gas (a mixture of carbon monoxide and hydrogen). Synthesis gas, or syngas, is produced primarily by the reaction of natural gas, which is principally methane, with steam. It can also be produced by gasification of coal, petroleum coke, or biomass. The availability of syngas from coal gasification is expected to increase significantly in the future because of increasing development of integrated gasification combined cycle (IGCC) power generation. Because of the abundance of remote natural gas, the advent of IGCC, and environmental advantages, C1 chemistry is expected to become a major area of interest for the transportation fuel and chemical industries in the relatively near future. The CFFLS will therefore perform a valuable national service by providing science and engineering graduates that are trained in this important area. Syngas is the source of most hydrogen. Approximately 10 trillion standard cubic feet (SCF) of hydrogen are manufactured annually in the world. Most of this hydrogen is currently used for the production of ammonia and in a variety of refining and chemical operations. However, utilization of hydrogen in fuel cells is expected to grow significantly in the next century. Syngas is also the feedstock for all methanol and Fischer-Tropsch plants. Currently, world consumption of methanol is over 25 million tons per year. There are many methanol plants in the U.S. and throughout the world. Methanol and oxygenated transportation fuel products play a significant role in the CFFLS C1 program. Currently, the only commercial Fischer-Tropsch plants are overseas, principally in South Africa (SASOL). However, new plants are being built or planned for a number of locations. One possible location for future F

  11. Creswell's Energy Efficient Construction Program: A Big Project for a Small School.

    ERIC Educational Resources Information Center

    Kelsh, Bruce

    1982-01-01

    In Creswell (Oregon) High School's award winning vocational education program, students study energy efficient construction along with basic building skills. Part of the program has been the active recruitment of female, minority, disadvantaged, and handicapped students into the vocational area. Students have assembled solar hot water collectors,…

  12. Constructing a Self-Funded Program Takes More than Just Dollars and Cents

    ERIC Educational Resources Information Center

    Burke, Scott

    2012-01-01

    With a little ingenuity and a lot of dedication, the author created a self-funded construction program that is weathering the ups and downs of school funding; it enjoys great support from the community, is accomplishing more with less, and collaborative efforts between teachers are paying off. Creating such a program takes time, vision,…

  13. 32 CFR 1906.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... alterations. 1906.151 Section 1906.151 National Defense Other Regulations Relating to National Defense CENTRAL INTELLIGENCE AGENCY ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE CENTRAL INTELLIGENCE AGENCY § 1906.151 Program accessibility: New construction...

  14. 32 CFR 1906.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... alterations. 1906.151 Section 1906.151 National Defense Other Regulations Relating to National Defense CENTRAL INTELLIGENCE AGENCY ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE CENTRAL INTELLIGENCE AGENCY § 1906.151 Program accessibility: New construction...

  15. 32 CFR 1906.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... alterations. 1906.151 Section 1906.151 National Defense Other Regulations Relating to National Defense CENTRAL INTELLIGENCE AGENCY ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE CENTRAL INTELLIGENCE AGENCY § 1906.151 Program accessibility: New construction...

  16. 32 CFR 1906.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... alterations. 1906.151 Section 1906.151 National Defense Other Regulations Relating to National Defense CENTRAL INTELLIGENCE AGENCY ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE CENTRAL INTELLIGENCE AGENCY § 1906.151 Program accessibility: New construction...

  17. 75 FR 60263 - Federal Acquisition Regulation; Offering a Construction Requirement-8(a) Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-29

    ... Federal Acquisition Regulation; Offering a Construction Requirement--8(a) Program AGENCIES: Department of... Regulations Council (Councils) are issuing a final rule amending the Federal Acquisition Regulation (FAR) to revise FAR subpart 19.8, Contracting with the Small Business Administration (The 8(a) Program),...

  18. Highway Safety Program Manual: Volume 12: Highway Design, Construction and Maintenance.

    ERIC Educational Resources Information Center

    National Highway Traffic Safety Administration (DOT), Washington, DC.

    Volume 12 of the 19-volume Highway Safety Program Manual (which provides guidance to State and local governments on preferred highway safety practices) focuses on highway design, construction and maintenance. The purpose and specific objectives of such a program are described. Federal authority in the area of highway safety and policies regarding…

  19. 45 CFR 606.52 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to 101-19.607, apply to buildings... SCIENCE FOUNDATION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE NATIONAL SCIENCE FOUNDATION § 606.52 Program accessibility: New construction...

  20. 22 CFR 1600.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 22 Foreign Relations 2 2011-04-01 2009-04-01 true Program accessibility: New construction and alterations. 1600.151 Section 1600.151 Foreign Relations JAPAN-UNITED STATES FRIENDSHIP COMMISSION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE...

  1. Item Pool Construction Using Mixed Integer Quadratic Programming (MIQP). GMAC® Research Report RR-14-01

    ERIC Educational Resources Information Center

    Han, Kyung T.; Rudner, Lawrence M.

    2014-01-01

    This study uses mixed integer quadratic programming (MIQP) to construct multiple highly equivalent item pools simultaneously, and compares the results from mixed integer programming (MIP). Three different MIP/MIQP models were implemented and evaluated using real CAT item pool data with 23 different content areas and a goal of equal information…

  2. 45 CFR 606.52 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to 101-19.607, apply to buildings... SCIENCE FOUNDATION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE NATIONAL SCIENCE FOUNDATION § 606.52 Program accessibility: New construction...

  3. 45 CFR 606.52 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to 101-19.607, apply to buildings... SCIENCE FOUNDATION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE NATIONAL SCIENCE FOUNDATION § 606.52 Program accessibility: New construction...

  4. LEAF: A Microcomputer Program for Constructing the Tukey Stem and Leaf Graph.

    ERIC Educational Resources Information Center

    Pascale, Pietro J.; Smith, Joseph

    1986-01-01

    This paper presents a BASIC microcomputer program that constructs the Tukey (1977) stem and leaf graph. Options within the LEAF program include a modified stem and leaf where the stem is split and a parallel stem and leaf graph where two separate sets of data are displayed from a common stem. (Author)

  5. 45 CFR 606.52 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... SCIENCE FOUNDATION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE NATIONAL SCIENCE FOUNDATION § 606.52 Program accessibility: New construction and... usable by individuals with handicaps. The definitions, requirements, and standards of the...

  6. 45 CFR 606.52 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... SCIENCE FOUNDATION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE NATIONAL SCIENCE FOUNDATION § 606.52 Program accessibility: New construction and... usable by individuals with handicaps. The definitions, requirements, and standards of the...

  7. 32 CFR 1906.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Barriers Act of 1968 (42 U.S.C. 4151-4175), as established in 41 CFR 101-19.600 to 101-19-607, apply to... INTELLIGENCE AGENCY ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE CENTRAL INTELLIGENCE AGENCY § 1906.151 Program accessibility: New construction...

  8. Software for C1 interpolation

    NASA Technical Reports Server (NTRS)

    Lawson, C. L.

    1977-01-01

    The problem of mathematically defining a smooth surface, passing through a finite set of given points is studied. Literature relating to the problem is briefly reviewed. An algorithm is described that first constructs a triangular grid in the (x,y) domain, and first partial derivatives at the modal points are estimated. Interpolation in the triangular cells using a method that gives C sup.1 continuity overall is examined. Performance of software implementing the algorithm is discussed. Theoretical results are presented that provide valuable guidance in the development of algorithms for constructing triangular grids.

  9. Accurate construction of consensus genetic maps via integer linear programming.

    PubMed

    Wu, Yonghui; Close, Timothy J; Lonardi, Stefano

    2011-01-01

    We study the problem of merging genetic maps, when the individual genetic maps are given as directed acyclic graphs. The computational problem is to build a consensus map, which is a directed graph that includes and is consistent with all (or, the vast majority of) the markers in the input maps. However, when markers in the individual maps have ordering conflicts, the resulting consensus map will contain cycles. Here, we formulate the problem of resolving cycles in the context of a parsimonious paradigm that takes into account two types of errors that may be present in the input maps, namely, local reshuffles and global displacements. The resulting combinatorial optimization problem is, in turn, expressed as an integer linear program. A fast approximation algorithm is proposed, and an additional speedup heuristic is developed. Our algorithms were implemented in a software tool named MERGEMAP which is freely available for academic use. An extensive set of experiments shows that MERGEMAP consistently outperforms JOINMAP, which is the most popular tool currently available for this task, both in terms of accuracy and running time. MERGEMAP is available for download at http://www.cs.ucr.edu/~yonghui/mgmap.html. PMID:20479505

  10. Accurate construction of consensus genetic maps via integer linear programming.

    PubMed

    Wu, Yonghui; Close, Timothy J; Lonardi, Stefano

    2011-01-01

    We study the problem of merging genetic maps, when the individual genetic maps are given as directed acyclic graphs. The computational problem is to build a consensus map, which is a directed graph that includes and is consistent with all (or, the vast majority of) the markers in the input maps. However, when markers in the individual maps have ordering conflicts, the resulting consensus map will contain cycles. Here, we formulate the problem of resolving cycles in the context of a parsimonious paradigm that takes into account two types of errors that may be present in the input maps, namely, local reshuffles and global displacements. The resulting combinatorial optimization problem is, in turn, expressed as an integer linear program. A fast approximation algorithm is proposed, and an additional speedup heuristic is developed. Our algorithms were implemented in a software tool named MERGEMAP which is freely available for academic use. An extensive set of experiments shows that MERGEMAP consistently outperforms JOINMAP, which is the most popular tool currently available for this task, both in terms of accuracy and running time. MERGEMAP is available for download at http://www.cs.ucr.edu/~yonghui/mgmap.html.

  11. MATES in construction: impact of a multimodal, community-based program for suicide prevention in the construction industry.

    PubMed

    Gullestrup, Jorgen; Lequertier, Belinda; Martin, Graham

    2011-11-01

    A large-scale workplace-based suicide prevention and early intervention program was delivered to over 9,000 construction workers on building sites across Queensland. Intervention components included universal General Awareness Training (GAT; general mental health with a focus on suicide prevention); gatekeeper training provided to construction worker volunteer 'Connectors'; Suicide First Aid (ASIST) training offered to key workers; outreach support provided by trained and supervised MIC staff; state-wide suicide prevention hotline; case management service; and postvention support provided in the event of a suicide. Findings from over 7,000 workers (April 2008 to November 2010) are reported, indicating strong construction industry support, with 67% building sites and employers approached agreeing to participate in MIC. GAT participants demonstrated significantly increased suicide prevention awareness compared with a comparison group. Connector training participants rated MIC as helpful and effective, felt prepared to intervene with a suicidal person, and knew where to seek help for a suicidal individual following the training. Workers engaged positively with the after-hours crisis support phone line and case management. MIC provided postvention support to 10 non-MIC sites and sites engaged with MIC, but not yet MIC-compliant. Current findings support the potential effectiveness and social validity of MIC for preventing suicide in construction workers.

  12. The enzymatic nature of C'1r. Conversion of C'1s to C'1 esterase and digestion of amino acid esters by C'1r.

    PubMed

    Naff, G B; Ratnoff, O S

    1968-10-01

    Human C'1, a macromolecular complex composed of three subunits, is the zymogen for at least two distinct enzymes. Preparations of one subunit, C'1r, functioned as a protease which converted another subunit, C'1s, to C'1 esterase. The conversion of C'1s to C'1 esterase by C'1r was blocked by Liquoid, phenyl methylsulfonyl fluoride, and calcium ions, but not by soybean trypsin inhibitor, hirudin, or heparin. Preparations of C'1r also possessed two additional functions, i.e., the ability to hydrolyze certain synthetic amino acid esters and to participate in immune hemolysis. Evidence was presented which indicates that these three functions are properties of a single entity, C'1r, but not of the same portion of its molecular structure. These observations suggest that C'1r has at least two active sites, one for its reaction with C'1q, an additional subunit of C'1, and one for its reaction with C'1s; together, the three subcomponents, C'1q, C'1r, and C'1s, form a single functional unit, the first component of complement.

  13. Supramolecular Differentiation for Construction of Anisotropic Fullerene Nanostructures by Time-Programmed Control of Interfacial Growth.

    PubMed

    Bairi, Partha; Minami, Kosuke; Hill, Jonathan P; Nakanishi, Waka; Shrestha, Lok Kumar; Liu, Chao; Harano, Koji; Nakamura, Eiichi; Ariga, Katsuhiko

    2016-09-27

    Supramolecular assembly can be used to construct a wide variety of ordered structures by exploiting the cumulative effects of multiple noncovalent interactions. However, the construction of anisotropic nanostructures remains subject to some limitations. Here, we demonstrate the preparation of anisotropic fullerene-based nanostructures by supramolecular differentiation, which is the programmed control of multiple assembly strategies. We have carefully combined interfacial assembly and local phase separation phenomena. Two fullerene derivatives, PhH and C12H, were together formed into self-assembled anisotropic nanostructures by using this approach. This technique is applicable for the construction of anisotropic nanostructures without requiring complex molecular design or complicated methodology. PMID:27541964

  14. Characterization of recombinant C1 inhibitor P1 variants.

    PubMed

    Eldering, E; Huijbregts, C C; Lubbers, Y T; Longstaff, C; Hack, C E

    1992-04-01

    Twelve human C1 inhibitor P1 variants were constructed by site-directed mutagenesis of the codon for arginine 444 and were expressed in COS-1 cells to analyze the functional properties. The ability to bind to target proteases, as well as potential substrate-like behavior, was investigated with radioimmunoassays. The P1-Lys variant retained binding capacity toward C1s, plasmin, and kallikrein. In addition, complex formation with C1s was detected for P1-Asn and P1-His. All other P1 substitutions resulted in C1 inhibitor variants that neither complexed with nor were inactivated by C1s, kallikrein, beta-factor XIIa, or plasmin. Electrophoretic studies confirmed that P1-Lys and P1-His can form sodium dodecyl sulfate-resistant complexes with C1s. In contrast, the C1s-P1-Asn complex dissociated upon addition of sodium dodecyl sulfate. Kinetic experiments by the method of progress curves generated association rate constants (kon) with C1s of 4.2 x 10(4) M-1 s-1 for recombinant wild-type C1 inhibitor and 1.7 x 10(4) M-1 s-1 for P1-Lys. For P1-Asn and P1-His, kon was decreased approximately 100-fold. The results from inhibition experiments were compatible with a model of reversible inhibition, although the observed dissociation rate for wild-type C1 inhibitor is too low (1-2 x 10(-6) s-1) to be physiologically relevant. The overall inhibition constant (Ki) was estimated to be 0.03 nM. With P1-Asn, reversible inhibition could be demonstrated directly upon dilution of preformed complexes; the observed dissociation rate constant was 3.2 x 10(-4) s-1; and Ki increased to approximately 380 nM. These findings are discussed in relation to inhibitor specificity and inhibition mechanism.

  15. Constructing Adult Literacies at a Local Literacy Tutor-Training Program

    ERIC Educational Resources Information Center

    Roderick, Ryan

    2013-01-01

    This study investigates how literacy was constructed at an adult literacy organization's volunteer tutor-training program. By drawing on qualitative analysis of training texts used during training, such as training evaluations, and data gathered from interviews with experienced tutors, it is possible to identify the assumptions about literacy…

  16. 75 FR 9392 - National Institute of Standards and Technology Construction Grant Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-02

    ... Cooperative Agreements, which are contained in the Federal Register Notice of February 11, 2008 (73 FR 7696-01..., ``Federal Financial Report,'' as required by the Office of Management and Budget (OMB) (73 FR 61175, October... (PMP) for construction of the research science building/facility (25 percent). The program...

  17. 6 CFR 15.51 - Program accessibility; new construction and alterations.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 through 101-19.607 apply to buildings... alterations. 15.51 Section 15.51 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY... DEPARTMENT OF HOMELAND SECURITY § 15.51 Program accessibility; new construction and alterations....

  18. Library Programs. Public Library Construction: An Overview and Analysis. Fiscal Year 1987.

    ERIC Educational Resources Information Center

    Fork, Donald J.

    Since it was first authorized as an amendment to the Library Services Act (LSCA) in 1964, the LSCA Title II program has supported projects which have resulted in obligations of more than $28.5 million in federal funds for public library construction through fiscal year 1987. During its long legislative history, which has seen many changes and…

  19. 45 CFR 1803.8 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19... 45 Public Welfare 4 2010-10-01 2010-10-01 false Program accessibility: New construction and alterations. 1803.8 Section 1803.8 Public Welfare Regulations Relating to Public Welfare (Continued) HARRY...

  20. 40 CFR 12.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ....C. 4151-4157), as established in 41 CFR 101-19.600 to 101-19.607, apply to buildings covered by this... 40 Protection of Environment 1 2011-07-01 2011-07-01 false Program accessibility: New construction and alterations. 12.151 Section 12.151 Protection of Environment ENVIRONMENTAL PROTECTION...

  1. 40 CFR 12.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ....C. 4151-4157), as established in 41 CFR 101-19.600 to 101-19.607, apply to buildings covered by this... 40 Protection of Environment 1 2013-07-01 2013-07-01 false Program accessibility: New construction and alterations. 12.151 Section 12.151 Protection of Environment ENVIRONMENTAL PROTECTION...

  2. 45 CFR 1181.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... established in 41 CFR 101-19.600 to 101-19.607, apply to buildings covered by this section. ...) NATIONAL FOUNDATION ON THE ARTS AND THE HUMANITIES INSTITUTE OF MUSEUM AND LIBRARY SERVICES ENFORCEMENT OF... LIBRARY SERVICES § 1181.151 Program accessibility: New construction and alterations. Each building or...

  3. The Laborers-AGC Construction Skills Training Program. Final Performance Report.

    ERIC Educational Resources Information Center

    Tippie, John L.; Rice, Eric

    Patterned after a previously successful Laborers-Associated General Contractors model named the Construction Skills Training Program, a demonstration project was implemented at five regional training centers. At least eight courses were created, combined, or revised. Four full-length audiovisual support pieces were completed. Three courses were…

  4. 29 CFR 1615.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... THE EQUAL EMPLOYMENT OPPORTUNITY COMMISSION AND IN ACCESSIBILITY OF COMMISSION ELECTRONIC AND INFORMATION TECHNOLOGY § 1615.151 Program accessibility: New construction and alterations. Each building or....S.C. 4151-4157), as established in 41 CFR subpart 101-19.6, apply to buildings covered by...

  5. Planning Construction Projects: An Evaluation Report for the Occupational Exploration Program.

    ERIC Educational Resources Information Center

    Altschuld, James W.; Pritz, Sandra

    The evaluation report is one of seven produced for the Occupational Exploration Program (OEP), a series of simulated occupational experiences designed for junior high school students. Describing the pilot testing of the simulation dealing with construction, the report contains sections describing the simulation context, evaluation procedures,…

  6. State Plan for Library Programs Under Library Services and Construction Act as Amended.

    ERIC Educational Resources Information Center

    Hawaii State Dept. of Education, Honolulu. Office of Library Services.

    Hawaii's State Plan for Library Programs under the Library Services and Construction Act includes the following sections: (1.0) General Provisions; (1.1) State Agency (exhibits are: Executive Order No. 19, and the Constitution of the State of Hawaii); (1.2) Free Library Services; (1.3) Tables of Organization; (1.4) Personnel; (1.5) Custody of…

  7. Programming for the Internet and Experiential Learning: A New Approach Incorporating a Constructed World

    ERIC Educational Resources Information Center

    Taylor, Adam P. R.

    2007-01-01

    This paper reports on a novel approach taken by the author to the teaching of an Internet programming course targeted at final year diploma and second year degree students. The uniqueness of engineers and their identity is discussed, as is the self-constructed world and ways of knowing in which humankind operates. The novel approach to teaching a…

  8. Constructing Reading: Building Conceptions of Literacy in a Volunteer Read-Aloud Program

    ERIC Educational Resources Information Center

    Dawes, Erika Thulin

    2007-01-01

    This study describes the interactions of three adult-child reading partnerships in a program that matches corporate volunteers with "at risk" students for weekly hour-long story reading sessions. Using discourse analysis within a sociocultural framework, the researcher identified variation in the way these partners were constructing the act and…

  9. Environmental Assessment for the Construction and Operation of the Constellation Program A-3 Test Stand

    NASA Technical Reports Server (NTRS)

    Kennedy, Carolyn D.

    2007-01-01

    This document is an environmental assessment that examines the environmental impacts of a proposed plan to clear land and to construct a test stand for use in testing the J-2X rocket engine at simulated altitude conditions in support of NASA's Constellation Program.

  10. A Scientist or Salesman? Identity Construction through Referent Honorifics on a Japanese Shopping Channel Program

    ERIC Educational Resources Information Center

    Cook, Haruko Minegishi

    2013-01-01

    This paper explores how referent honorifics contribute to identity construction on a Japanese TV shopping channel program. Drawing on Ochs' twostep model of indexicality (1993, 1996) and Agah's proposal (1993) that honorifics are not directly linked to social status but index a "relative position within events of discursive…

  11. 45 CFR 1803.8 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ..., and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.... TRUMAN SCHOLARSHIP FOUNDATION NONDISCRIMINATION ON THE BASIS OF HANDICAP § 1803.8 Program accessibility... by, on behalf of, or for the use of the Foundation shall be designed, constructed, or altered so...

  12. 45 CFR 1803.8 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ..., and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.... TRUMAN SCHOLARSHIP FOUNDATION NONDISCRIMINATION ON THE BASIS OF HANDICAP § 1803.8 Program accessibility... by, on behalf of, or for the use of the Foundation shall be designed, constructed, or altered so...

  13. 45 CFR 1803.8 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ..., and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.... TRUMAN SCHOLARSHIP FOUNDATION NONDISCRIMINATION ON THE BASIS OF HANDICAP § 1803.8 Program accessibility... by, on behalf of, or for the use of the Foundation shall be designed, constructed, or altered so...

  14. 45 CFR 1803.8 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ..., and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.... TRUMAN SCHOLARSHIP FOUNDATION NONDISCRIMINATION ON THE BASIS OF HANDICAP § 1803.8 Program accessibility... by, on behalf of, or for the use of the Foundation shall be designed, constructed, or altered so...

  15. 22 CFR 1600.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    .... 4151-4157), as established in 41 CFR 101-19.600 to 101-19.607, apply to buildings covered by this... alterations. 1600.151 Section 1600.151 Foreign Relations JAPAN-UNITED STATES FRIENDSHIP COMMISSION ENFORCEMENT... STATES FRIENDSHIP COMMISSION § 1600.151 Program accessibility: New construction and alterations....

  16. 22 CFR 1600.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    .... 4151-4157), as established in 41 CFR 101-19.600 to 101-19.607, apply to buildings covered by this... alterations. 1600.151 Section 1600.151 Foreign Relations JAPAN-UNITED STATES FRIENDSHIP COMMISSION ENFORCEMENT... STATES FRIENDSHIP COMMISSION § 1600.151 Program accessibility: New construction and alterations....

  17. 22 CFR 1600.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    .... 4151-4157), as established in 41 CFR 101-19.600 to 101-19.607, apply to buildings covered by this... alterations. 1600.151 Section 1600.151 Foreign Relations JAPAN-UNITED STATES FRIENDSHIP COMMISSION ENFORCEMENT... STATES FRIENDSHIP COMMISSION § 1600.151 Program accessibility: New construction and alterations....

  18. Dora's Program: A Constructively Marginalized Paraeducator and Her Developmental Biliteracy Program

    ERIC Educational Resources Information Center

    Lucero, Audrey

    2010-01-01

    This article discusses findings from a case study of one elementary bilingual paraeducator, highlighting how the recognition of situated cultural capital enabled her to move from traditional to constructive marginality. I argue that her actions, the actions of others, and conditions within the school enabled her to use culturally relevant funds of…

  19. Programming constructs for real-time distributed knowledge-based systems

    SciTech Connect

    Cromarty, A.S.

    1988-01-01

    This study presents a set of mechanisms for use in the construction of distributed knowledge-based systems that must meet such practical performance constraints. These mechanisms are made manifest in the form of programming-language constructs designed to support the development of DKBS's that implement a multiplicity of alternative policies and abstract architectures. The proposed constructs are studied, using two techniques: implementation in an experimental testbed environment and performance measurement in controlled experiments that assess the time cost of interagent communications among loosely-coupled LISP processes. The author's empirical data on interagent communications should prove valuable to DKBS implementors who must meet real-time constraints using contemporary hardware and software technology. It is concluded that it is possible to construct interagent communications cost models that are useful and have a high degree of predictive value. Experimental results indicate that the cost of message-based interagent communications between symbolic processing agents is very high.

  20. Military construction program economic analysis manual: Text and appendixes: Hazardous Waste Remedial Actions Program. [Contains glossary

    SciTech Connect

    Not Available

    1987-12-01

    This manual enables the US Air Force to comprehensively and systematically analyze alternative approaches to meeting its military construction requirements. The manual includes step-by-step procedures for completing economic analyses for military construction projects, beginning with determining if an analysis is necessary. Instructions and a checklist of the tasks involved for each step are provided; and examples of calculations and illustrations of completed forms are included. The manual explains the major tasks of an economic analysis, including identifying the problem, selecting realistic alternatives for solving it, formulating appropriate assumptions, determining the costs and benefits of the alternatives, comparing the alternatives, testing the sensitivity of major uncertainties, and ranking the alternatives. Appendixes are included that contain data, indexes, and worksheets to aid in performing the economic analyses. For reference, Volume 2 contains sample economic analyses that illustrate how each form is filled out and that include a complete example of the documentation required. 6 figs., 12 tabs.

  1. 25 CFR 1000.240 - What construction programs included in an AFA are subject to this subpart?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... procurement except that all project design and actual construction activities are subject to all the... AFA are subject to this subpart. This includes design, construction, repair, improvement, expansion... Tribal, facilities and projects. (b) The following programs and activities are not construction...

  2. 25 CFR 1000.240 - What construction programs included in an AFA are subject to this subpart?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... procurement except that all project design and actual construction activities are subject to all the... AFA are subject to this subpart. This includes design, construction, repair, improvement, expansion... Tribal, facilities and projects. (b) The following programs and activities are not construction...

  3. 25 CFR 1000.240 - What construction programs included in an AFA are subject to this subpart?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... procurement except that all project design and actual construction activities are subject to all the... AFA are subject to this subpart. This includes design, construction, repair, improvement, expansion... Tribal, facilities and projects. (b) The following programs and activities are not construction...

  4. 25 CFR 1000.240 - What construction programs included in an AFA are subject to this subpart?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... procurement except that all project design and actual construction activities are subject to all the... AFA are subject to this subpart. This includes design, construction, repair, improvement, expansion... Tribal, facilities and projects. (b) The following programs and activities are not construction...

  5. 25 CFR 1000.240 - What construction programs included in an AFA are subject to this subpart?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... procurement except that all project design and actual construction activities are subject to all the... AFA are subject to this subpart. This includes design, construction, repair, improvement, expansion... Tribal, facilities and projects. (b) The following programs and activities are not construction...

  6. Predators of knowledge construction: Interpreting students' metacognition in an amusement park physics program

    NASA Astrophysics Data System (ADS)

    Anderson, David; Nashon, Samson

    2007-03-01

    It is recognized widely that learning is a dynamic and idiosyncratic process of construction and reconstruction of concepts in response to new experiences. It is influenced by the learner's prior knowledge, motivation, and sociocultural context. This study investigated how year 11 and 12 physics students' metacognition influences the development of their conceptual understandings of kinematics. An interpretive case study approach was used to investigate students working in collaborative groups in the context of an amusement park physics program. The metacognitive character of individual learners was demonstrated to have a strong influence on their conceptual development. Moreover, the metacognitive character of individuals within the small group contexts investigated was a key factor influencing the groups' collective knowledge development. A coyote-rabbit metaphor was developed to interpret the resilience and weaknesses of individual and group knowledge construction processes, and elucidates new theoretical understandings regarding metacognition and its influence on knowledge construction.

  7. Curtiss SB2C-1 Helldiver

    NASA Technical Reports Server (NTRS)

    1944-01-01

    Curtiss SB2C-1 Helldiver: This Curtiss SB2C-1 Helldiver was flown by the NACA at Langley with an early radio-control system. Flying controls such as a spring tab were also examined with this airframe. Helldivers were also operated by the U. S. Army as A-25 Shrikes.

  8. C1 neurons: the body's EMTs.

    PubMed

    Guyenet, Patrice G; Stornetta, Ruth L; Bochorishvili, Genrieta; Depuy, Seth D; Burke, Peter G R; Abbott, Stephen B G

    2013-08-01

    The C1 neurons reside in the rostral and intermediate portions of the ventrolateral medulla (RVLM, IVLM). They use glutamate as a fast transmitter and synthesize catecholamines plus various neuropeptides. These neurons regulate the hypothalamic pituitary axis via direct projections to the paraventricular nucleus and regulate the autonomic nervous system via projections to sympathetic and parasympathetic preganglionic neurons. The presympathetic C1 cells, located in the RVLM, are probably organized in a roughly viscerotopic manner and most of them regulate the circulation. C1 cells are variously activated by hypoglycemia, infection or inflammation, hypoxia, nociception, and hypotension and contribute to most glucoprivic responses. C1 cells also stimulate breathing and activate brain stem noradrenergic neurons including the locus coeruleus. Based on the various effects attributed to the C1 cells, their axonal projections and what is currently known of their synaptic inputs, subsets of C1 cells appear to be differentially recruited by pain, hypoxia, infection/inflammation, hemorrhage, and hypoglycemia to produce a repertoire of stereotyped autonomic, metabolic, and neuroendocrine responses that help the organism survive physical injury and its associated cohort of acute infection, hypoxia, hypotension, and blood loss. C1 cells may also contribute to glucose and cardiovascular homeostasis in the absence of such physical stresses, and C1 cell hyperactivity may contribute to the increase in sympathetic nerve activity associated with diseases such as hypertension. PMID:23697799

  9. Relating Women's Experience and Skills to Competencies Required in Selected Occupations and Vocational Education Programs. Project Report, Task C1, Revised Competency Analysis: Credentialing Women's Life Experiences (Project ACCESS).

    ERIC Educational Resources Information Center

    Ekstrom, Ruth B.; And Others

    Competency scales were developed for 10 direct-entry occupations and 10 vocational education programs as part of Project ACCESS, a project to assess women's life experience learning for entrance into employment and for advanced placement in vocational education programs. Development of the occupational scales involved employer interviews, followed…

  10. Structure and function of the serine-protease subcomponents of C1: protein engineering studies.

    PubMed

    Gál, P; Závodszky, P

    1998-08-01

    Our protein engineering studies on human C1r and C1s revealed important characteristics of the individual domains of these multidomain serine-proteases, and supplied evidence about the cooperation of the domains to create binding sites, and to control the activation process. We expressed the recombinant subcomponents in the baculovirus-insect cell system and checked the biological activity. Deletions and point mutants of C1r were constructed and C1r-C1s chimeras were also produced. Our deletion mutants demonstrated that the N-terminal CUB domain and the EGF-like domain of C1r together are responsible for the calcium dependent C1r-C1s interaction. It seems very likely that these two modules form the calcium-binding site of the C1r alpha-fragment and participate in the tetramer formation. The deletion mutants also demonstrated that the N-terminal region of the C1r molecule contains essential elements involved in the control of activation of the serine-protease module. The substrate specificity of the serine-protease is also determined by the five N-terminal noncatalytic domain of C1r/C1s chimera, which contains the catalytic domain of C1s preceded by the N-terminal region of C1r, could replace the C1r in the hemolytically active C1 complex. The C1s/C1r chimera, in which the alpha-fragment of the C1r was replaced for that of the C1s exibits both C1r- and C1s-like characteristics. We stabilized the zymogen form of human C1r by mutating the Arg(463)-Ile(464) bond. Using our stable zymogen C1r we showed that one active C1r in the C1 complex is sufficient for the full activity of the entire complex. Further experiment with this mutant could provide us with important information about the structure of the C1 complex. PMID:9777415

  11. Structure and function of C1-inhibitor.

    PubMed

    Wagenaar-Bos, Ineke G A; Hack, C Erik

    2006-11-01

    C1-INH belongs to the family of serpins. Structural studies have yielded a clear understanding of the biochemical principle underlying the functional activities of these proteins. Although the crystal structure of C1-INH has yet to be revealed, homology modeling has provided a three-dimensional model of the serpin part of C1-INH. This model has helped us understand the biochemical consequences of mutations of the C1-INH gene as they occur in patients who have HAE. The structure of the N-terminal domain of C1-INH remains unknown; however, this part of the molecule is unlikely to be important in the inhibitory activity of C1-INH toward its target proteases. Mutations in this part have not been described in patients who have HAE, except for a deletion containing two cysteine residues involved in the stabilization of the serpin domain. Recent studies suggest some anti-inflammatory functions for this N-terminal part, possibly explaining the effects of C1-INH in diseases other than HAE.

  12. Construction safety program for the National Ignition Facility, July 30, 1999

    SciTech Connect

    Benjamin, D W

    1999-07-30

    The Construction Safety Program (CSP) for NIF sets forth the responsibilities, guidelines, rules, policies and regulations for all workers involved in the construction, special equipment installation, acceptance testing, and start-up of NIF at LLNL during the construction period of NIF. During this period, all workers are required to implement measures to create a universal awareness which promotes safe practice at the work site, and which will achieve NIF's management objectives in preventing accidents and illnesses. Construction safety for NIF is predicated on everyone performing their jobs in a manner that prevents job-related disabling injuries and illnesses. Integrated Safety Management (ISM) is practiced in the execution of all activities associated with the NIF Project. The seven Principles of ISM are: (1) Line management is responsible for safety. (2) Clear roles and responsibilities are established and maintained. (3) Personnel possess competence commensurate with responsibilities. (4) Resource allocations are balanced, making ES and H a priority in project planning and execution. (5) Safety requirements are identified and implemented. (6) Hazard controls are tailored to the project work. (7) Operations are authorized before work begins.

  13. Northwest Energy Efficient Manufactured Housing Program: High Performance Manufactured Home Prototyping and Construction Development

    SciTech Connect

    Hewes, Tom; Peeks, Brady

    2013-11-01

    The Building America Partnership for Improved Residential Construction, the Bonneville Power Administration (BPA), and Northwest Energy Works (NEW), the current Northwest Energy Efficient Manufactured Housing Program (NEEM) administrator, have been collaborating to conduct research on new specifications that would improve on the energy requirements of a NEEM home. In its role as administrator, NEW administers the technical specs, performs research and engineering analysis, implements ongoing construction quality management procedures, and maintains a central database with home tracking. This project prototyped and assessed the performances of cost-effective high performance building assemblies and mechanical systems that are not commonly deployed in the manufacturing setting. The package of measures is able to reduce energy used for space conditioning, water heating and lighting by 50% over typical manufactured homes produced in the northwest.

  14. Northwest Energy Efficient Manufactured Housing Program: High Performance Manufactured Home Prototyping and Construction Development

    SciTech Connect

    Hewes, T.; Peeks, B.

    2013-11-01

    The Building America Partnership for Improved Residential Construction, the Bonneville Power Administration (BPA), and Northwest Energy Works (NEW), the current Northwest Energy Efficient Manufactured Housing Program (NEEM) administrator, have been collaborating to conduct research on new specifications that would improve on the energy requirements of a NEEM home. In its role as administrator, NEW administers the technical specs, performs research and engineering analysis, implements ongoing construction quality management procedures, and maintains a central database with home tracking. This project prototyped and assessed the performances of cost-effective high performance building assemblies and mechanical systems that are not commonly deployed in the manufacturing setting. The package of measures is able to reduce energy used for space conditioning, water heating and lighting by 50 percent over typical manufactured homes produced in the northwest.

  15. Proteasome-mediated degradation antagonizes critical levels of the apoptosis-inducing C1D protein

    PubMed Central

    Rothbarth, Karsten; Stammer, Hermann; Werner, Dieter

    2002-01-01

    The C1D gene is expressed in a broad spectrum of mammalian cells and tissues but its product induces apoptotic cell death when exceeding a critical level. Critical levels are achieved in a fraction of cells by transient transfection with EGFP-tagged C1D expression constructs. However, transfected cells expressing sub-critical levels of C1D(EGFP) escape apoptotic cell death by activation of a proteasome-mediated rescue mechanism. Inhibition of the proteasome-dependent degradation of the C1D(EGFP) protein results in a parallel increase of the intracellular C1D level and in the fraction of apoptotic cells. PMID:12379155

  16. Curtiss SB2C-1 Helldiver

    NASA Technical Reports Server (NTRS)

    1943-01-01

    Curtiss SB2C-1 Helldiver: The front canopy came off this Curtiss SB2C-1 Helldiver while it was in flight, injuring pilot Herbert H. Hoover, but he recovered the plane back to the NACA at Langley Field. SB2Cs were license-built by Fairchild as SBFs and by Canadian Car and Foundry as SBWs. Helldivers were also flown by the British and Australians.

  17. 29 CFR 2575.502c-1 - Adjusted civil penalty under section 502(c)(1).

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... ACT OF 1974 ADJUSTMENT OF CIVIL PENALTIES UNDER ERISA TITLE I Adjustment of Civil Penalties Under ERISA Title I § 2575.502c-1 Adjusted civil penalty under section 502(c)(1). In accordance with the... section 502(c)(1) of the Employee Retirement Income Security Act of 1974, as amended (ERISA), is...

  18. 29 CFR 2575.502c-1 - Adjusted civil penalty under section 502(c)(1).

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... ACT OF 1974 ADJUSTMENT OF CIVIL PENALTIES UNDER ERISA TITLE I Adjustment of Civil Penalties Under ERISA Title I § 2575.502c-1 Adjusted civil penalty under section 502(c)(1). In accordance with the... section 502(c)(1) of the Employee Retirement Income Security Act of 1974, as amended (ERISA), is...

  19. 29 CFR 2575.502c-1 - Adjusted civil penalty under section 502(c)(1).

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... ACT OF 1974 ADJUSTMENT OF CIVIL PENALTIES UNDER ERISA TITLE I Adjustment of Civil Penalties Under ERISA Title I § 2575.502c-1 Adjusted civil penalty under section 502(c)(1). In accordance with the... section 502(c)(1) of the Employee Retirement Income Security Act of 1974, as amended (ERISA), is...

  20. 29 CFR 2575.502c-1 - Adjusted civil penalty under section 502(c)(1).

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... ACT OF 1974 ADJUSTMENT OF CIVIL PENALTIES UNDER ERISA TITLE I Adjustment of Civil Penalties Under ERISA Title I § 2575.502c-1 Adjusted civil penalty under section 502(c)(1). In accordance with the... section 502(c)(1) of the Employee Retirement Income Security Act of 1974, as amended (ERISA), is...

  1. 29 CFR 2575.502c-1 - Adjusted civil penalty under section 502(c)(1).

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... ACT OF 1974 ADJUSTMENT OF CIVIL PENALTIES UNDER ERISA TITLE I Adjustment of Civil Penalties Under ERISA Title I § 2575.502c-1 Adjusted civil penalty under section 502(c)(1). In accordance with the... section 502(c)(1) of the Employee Retirement Income Security Act of 1974, as amended (ERISA), is...

  2. EPA construction grants: Information on the Milwaukee Area Sewerage System Improvement Program

    SciTech Connect

    Not Available

    1986-01-01

    The Milwaukee Sewerage Water Pollution Abatement Program was initiated in response to federal and state court lawsuits to halt Lake Michigan's pollution. It is about $360 million under budget and on schedule with a 1996 target completion date. GAO found that the district meets the federal criteria for an eligible grantee; federal and Wisconsin regulations permit architect and engineering contracts to be competitively negotiated and do not require advertised competitive bidding; the job site rule, which limits allowable overhead billings, was not applied because it was determined that the operation of the prime contractor responsible for managing the program and its technical integrity did not constitute a job site; no federal funds have been involved in the disposition of rock from the north shore project site; and the total estimated architect and engineering costs $344.7 million represent about 28 percent of total estimated construction costs.

  3. Employers' Perception of Graduates with Entry-Level Technical Skills from Construction Industry Programs in Ghana and Nigeria

    ERIC Educational Resources Information Center

    Acheampong, Philip

    2013-01-01

    The purpose of this comparative study was to identify the technical skills and abilities needed by prospective employees of construction industries in Ghana and Nigeria. Potential employees were defined here as recent graduates of construction industry programs with entry-level technical skills. The continuous growth in and expansion of these two…

  4. In-space assembly and construction technology project summary: Infrastructure operations area of the operations technology program

    NASA Technical Reports Server (NTRS)

    Bush, Harold

    1991-01-01

    Viewgraphs describing the in-space assembly and construction technology project of the infrastructure operations area of the operation technology program are presented. Th objective of the project is to develop and demonstrate an in-space assembly and construction capability for large and/or massive spacecraft. The in-space assembly and construction technology program will support the need to build, in orbit, the full range of spacecraft required for the missions to and from planet Earth, including: earth-orbiting platforms, lunar transfer vehicles, and Mars transfer vehicles.

  5. 25 CFR 170.404 - What happens when a tribe uses its IRR Program construction funds for transportation planning?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false What happens when a tribe uses its IRR Program construction funds for transportation planning? 170.404 Section 170.404 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR LAND AND WATER INDIAN RESERVATION ROADS PROGRAM Planning, Design, and...

  6. Construction of Engineering Education Program based on the Alumni's Evaluation of the Educational Outcome

    NASA Astrophysics Data System (ADS)

    Tsukamoto, Takehiko; Nishizawa, Hitoshi

    The Department of Electrical and Electronic Engineering in Toyota National College of Technology has put great emphasis on fundamental subjects, such as “electrical and electronic circuit" and “electromagnetism" more than 40 years. On the other hand, several issues of our college were clarified by the alumni's evaluation of the educational outcome in 2002. The most serious issue was low achievement of English and Social education. The alumni of all generation are dissatisfied with their low skill in English communication. As a part of the educational reforms, our department has constructed a new engineering education program focusing on fundamental ability. We introduced many problem-based-learning experiments and the compulsory subjects such as “English communication for electrical engineers" and “Engineering Ethics" into this program. Great educative results are obtained by these improvements. As a typical example, the scores of all 2nd grade students of advanced engineering course in TOEIC tests became 450 points or more. Our program has been authorized by JABEE since 2004.

  7. Microwave spectra for the three 13C1 isotopologues of propene and new rotational constants for propene and its 13C1 isotopologues

    NASA Astrophysics Data System (ADS)

    Craig, Norman C.; Groner, Peter; Conrad, Andrew R.; Gurusinghe, Ranil; Tubergen, Michael J.

    2016-10-01

    New measurements of microwave lines (A and E) of propene and its three 13C1 isotopologues have been made in the 10-22 GHz region with FT accuracy. The revised lines for propene along with many hundreds from the literature were fitted with the ERHAM program for internal rotors to give improved rotational constants. The new constants are A0 = 46280.2904(16), B0 = 9305.24260(30), and C0 = 8134.22685(28) MHz. Lines for the 3-13C1 species were observed in a pure sample; lines for the 1-13C1 and 2-13C1 species were observed in natural abundance. In fitting the limited sets of lines for the 13C1 species, many of the centrifugal distortion constants and most of the tunneling parameters were transferred from the fit of propene itself with 27 parameters. Improved rotational constants for the 13C1 species are reported.

  8. The cultural construction of interdisciplinarity: Doctoral student socialization in an interdisciplinary neuroscience program

    NASA Astrophysics Data System (ADS)

    Holley, Karri A.

    Using the methodologies of individual and group interviews, observation, and document analysis, this dissertation examines the experiences of doctoral students enrolled in an interdisciplinary neuroscience program. A framework drawn from theories of organizational socialization is employed to understand the influence of an interdisciplinary program on doctoral student socialization. While abundant previous literature exists in regards to the socialization of doctoral students, such literature largely concentrates the disciplinary experience. The escalating import of globalization and shifting fiscal realities place new demands on Ph.D. programs and doctoral students to work as part of collaborative research teams, produce interdisciplinary knowledge, and integrate theory and practice. The increasing influence of such factors requires a new focus on interdisciplinarity and the changing Ph.D. The goal of this dissertation is to expand the existing framework of socialization by documenting the influence of such obstacles on knowledge acquisition, identity development, and professional investment. This study focuses on how interdisciplinary identities are constructed by doctoral students through individual interaction with the social environment and cultural context. Particular attention is given to the structural and cultural obstacles that doctoral students must negotiate as they navigate an interdisciplinary program. The study expands on the previous literature regarding doctoral student socialization by focusing on identity development, specifically a student's symbolic identity as a neuroscientist, a student's disciplinary identity (related to her professional background and undergraduate experiences), and a multi-disciplinary identity that allows for connections across disciplinary boundaries. In contrast to the traditional concepts of identity which focus on boundaries and differences as an inherent part of self-definition, the structure of identity advanced

  9. Some theoretical models and constructs generic to substance abuse prevention programs for adolescents: possible relevance and limitations for problem gambling.

    PubMed

    Evans, Richard I

    2003-01-01

    For the past several years the author and his colleagues have explored the area of how social psychological constructs and theoretical models can be applied to the prevention of health threatening behaviors in adolescents. In examining the need for the development of gambling prevention programs for adolescents, it might be of value to consider the application of such constructs and theoretical models as a foundation to the development of prevention programs in this emerging problem behavior among adolescents. In order to provide perspective to the reader, the present paper reviews the history of various psychosocial models and constructs generic to programs directed at prevention of substance abuse in adolescents. A brief history of some of these models, possibly most applicable to gambling prevention programs, are presented. Social inoculation, reasoned action, planned behavior, and problem behavior theory, are among those discussed. Some deficits of these models, are also articulated. How such models may have relevance to developing programs for prevention of problem gambling in adolescents is also discussed. However, the inherent differences between gambling and more directly health threatening behaviors such as substance abuse must, of course, be seriously considered in utilizing such models. Most current gambling prevention programs have seldom been guided by theoretical models. Developers of gambling prevention programs should consider theoretical foundations, particularly since such foundations not only provide a guide for programs, but may become critical tools in evaluating their effectiveness.

  10. A quenched c = 1 critical matrix model

    SciTech Connect

    Qiu, Zongan; Rey, Soo-Jong

    1990-12-01

    We study a variant of the Penner-Distler-Vafa model, proposed as a c = 1 quantum gravity: `quenched` matrix model with logarithmic potential. The model is exactly soluble, and exhibits a two-cut branching as observed in multicritical unitary matrix models and multicut Hermitian matrix models. Using analytic continuation of the power in the conventional polynomial potential, we also show that both the Penner-Distler-Vafa model and our `quenched` matrix model satisfy Virasoro algebra constraints.

  11. A quenched c = 1 critical matrix model

    SciTech Connect

    Qiu, Zongan; Rey, Soo-Jong.

    1990-12-01

    We study a variant of the Penner-Distler-Vafa model, proposed as a c = 1 quantum gravity: quenched' matrix model with logarithmic potential. The model is exactly soluble, and exhibits a two-cut branching as observed in multicritical unitary matrix models and multicut Hermitian matrix models. Using analytic continuation of the power in the conventional polynomial potential, we also show that both the Penner-Distler-Vafa model and our quenched' matrix model satisfy Virasoro algebra constraints.

  12. Notes on Liouville theory at c{<=}1

    SciTech Connect

    McElgin, Will

    2008-03-15

    The continuation of the Liouville conformal field theory to c{<=}1 is considered. The viability of an interpretation involving a timelike boson which is the conformal factor for two-dimensional asymptotically de Sitter geometries is examined. The conformal bootstrap leads to a three-point function with a unique analytic factor which is the same as that which appears along with the fusion coefficients in the minimal models. A corresponding nonanalytic factor produces a well-defined metric on fields only when the central charge is restricted to those of the topological minimal models, and when the conformal dimensions satisfy h>(c-1)/24. However, the theories considered here have a continuous spectrum which excludes the degenerate representations appearing in the minimal models. The c=1 theory has been investigated previously using similar techniques, and is identical to a nonrational conformal field theory (CFT) which arises as a limit of unitary minimal models. When coupled to unitary matter fields, the nonunitary theories with c{<=}-2 produce string amplitudes which are similar to those of the minimal string.

  13. Complement components C1r/C1s, bone morphogenic protein 1 and Xenopus laevis developmentally regulated protein UVS.2 share common repeats.

    PubMed

    Bork, P

    1991-04-22

    Property patterns were constructed, based on an alignment of related domains in human complement subcomponents C1r and C1s as well as in the sea urchin protein uEGF. This kind of consensus pattern was able to identify similar domains in a human bone morphogenic protein, in a Xenopus laevis embryonal protein involved in dorsoanterior development and in a calcium-dependent serine protease secreted from malignant hamster embryo fibroblast cells. Because of the high level of overall sequence homology this protease may be the hamsters' equivalent of the human complement subcomponent C1s. The resulting multiple alignment of all studied domains suggests functionally and structurally important regions.

  14. Accurate and interpretable nanoSAR models from genetic programming-based decision tree construction approaches.

    PubMed

    Oksel, Ceyda; Winkler, David A; Ma, Cai Y; Wilkins, Terry; Wang, Xue Z

    2016-09-01

    The number of engineered nanomaterials (ENMs) being exploited commercially is growing rapidly, due to the novel properties they exhibit. Clearly, it is important to understand and minimize any risks to health or the environment posed by the presence of ENMs. Data-driven models that decode the relationships between the biological activities of ENMs and their physicochemical characteristics provide an attractive means of maximizing the value of scarce and expensive experimental data. Although such structure-activity relationship (SAR) methods have become very useful tools for modelling nanotoxicity endpoints (nanoSAR), they have limited robustness and predictivity and, most importantly, interpretation of the models they generate is often very difficult. New computational modelling tools or new ways of using existing tools are required to model the relatively sparse and sometimes lower quality data on the biological effects of ENMs. The most commonly used SAR modelling methods work best with large datasets, are not particularly good at feature selection, can be relatively opaque to interpretation, and may not account for nonlinearity in the structure-property relationships. To overcome these limitations, we describe the application of a novel algorithm, a genetic programming-based decision tree construction tool (GPTree) to nanoSAR modelling. We demonstrate the use of GPTree in the construction of accurate and interpretable nanoSAR models by applying it to four diverse literature datasets. We describe the algorithm and compare model results across the four studies. We show that GPTree generates models with accuracies equivalent to or superior to those of prior modelling studies on the same datasets. GPTree is a robust, automatic method for generation of accurate nanoSAR models with important advantages that it works with small datasets, automatically selects descriptors, and provides significantly improved interpretability of models.

  15. A C1-inhibitor-complex assay (INCA): a method to detect C1 activation in vitro and in vivo.

    PubMed

    Hack, C E; Hannema, A J; Eerenberg-Belmer, A J; Out, T A; Aalberse, R C

    1981-10-01

    A radioimmunoassay (the C1-inhibitor-complex assay, INCA) is described for the detection of complexes that are composed of at least C1s and C1-inhibitor. This INCA is based on demonstrating that C1s and C1-inhibitor (C1-In) are linked: after an incubation with anti-C1s-Sepharose, bound C1sC1-In complexes are detected by 125I-anti-C1-In. C1sC1-In complexes were prepared by the addition of a slight excess of C1s to normal human serum (NHS). As little as 2 ng C1-In bound to C1s was detected. Additional free C1s in serum hardly influenced the detection of C1sC1-In complexes. Complexes presumably composed of C1rC1s(C1-In)2 were generated by the addition of aggregated IgG to NHS. This generation was inhibited by lowering the temperature to 0 degrees C, and by EDTA, and depended on the concentration of aggregated IgG. These complexes had a sedimentation value of approximately 9S. Complexes of C1s and C1-In were also generated in NHS by the addition of DNP-albumin and protein A, but not by zymosan. The INCA was applied to blood samples from normal donors and patients. Sixteen out of 19 samples from patients with acute glomerulonephritis contained increased amounts of C1rC1s(C1-In)2 complexes as compared with the amounts in blood samples from normal donors. The INCA provides a useful tool to assess the activation of C1 in the presence of C1-In, both in vitro and in vivo.

  16. Satellite Power System. Concept development and evaluation program, volume 6: Construction and operations

    NASA Technical Reports Server (NTRS)

    Benson, H.; Jenkins, L. M.

    1981-01-01

    The construction, operation, and maintenance requirements for a solar power satellite, including the space and ground systems, are reviewed. The basic construction guidelines are explained, and construction location options are discussed. The space construction tasks, equipment, and base configurations are discussed together with the operations required to place a solar power satellite in geosynchronous orbit. A rectenna construction technique is explained, and operation with the grid is defined. Maintenance requirements are summarized for the entire system. Key technology issues required for solar power satellite construction operations are defined.

  17. C1q protein binds to the apoptotic nucleolus and causes C1 protease degradation of nucleolar proteins.

    PubMed

    Cai, Yitian; Teo, Boon Heng Dennis; Yeo, Joo Guan; Lu, Jinhua

    2015-09-11

    In infection, complement C1q recognizes pathogen-congregated antibodies and elicits complement activation. Among endogenous ligands, C1q binds to DNA and apoptotic cells, but whether C1q binds to nuclear DNA in apoptotic cells remains to be investigated. With UV irradiation-induced apoptosis, C1q initially bound to peripheral cellular regions in early apoptotic cells. By 6 h, binding concentrated in the nuclei to the nucleolus but not the chromatins. When nucleoli were isolated from non-apoptotic cells, C1q also bound to these structures. In vivo, C1q exists as the C1 complex (C1qC1r2C1s2), and C1q binding to ligands activates the C1r/C1s proteases. Incubation of nucleoli with C1 caused degradation of the nucleolar proteins nucleolin and nucleophosmin 1. This was inhibited by the C1 inhibitor. The nucleoli are abundant with autoantigens. C1q binding and C1r/C1s degradation of nucleolar antigens during cell apoptosis potentially reduces autoimmunity. These findings help us to understand why genetic C1q and C1r/C1s deficiencies cause systemic lupus erythematosus.

  18. Microbial growth on C1 compounds

    PubMed Central

    Kemp, M. B.; Quayle, J. R.

    1967-01-01

    1. A study has been made of the incorporation of carbon from [14C]formaldehyde and [14C]formate by cultures of Pseudomonas methanica growing on methane. 2. The distribution of radioactivity within the non-volatile constituents of the ethanol-soluble fractions of the cells, after incubation with labelled compounds for periods of up to 1min., has been analysed by chromatography and radioautography. 3. Radioactivity was fixed from [14C]formaldehyde mainly into the phosphates of the sugars, glucose, fructose, sedoheptulose and allulose. 4. Very little radioactivity was fixed from [14C]formate; after 1min. the only products identified were serine and malate. 5. The distribution of radioactivity within the carbon skeleton of glucose, obtained from short-term incubations with [14C]methanol of Pseudomonas methanica growing on methane, has been investigated. At the earliest time of sampling over 70% of the radioactivity was located in C-1; as the time increased the radioactivity spread throughout the molecule. 6. The results have been interpreted in terms of a variant of the pentose phosphate cycle, involving the condensation of formaldehyde with C-1 of ribose 5-phosphate to give allulose phosphate. PMID:6030306

  19. Library Services for Indian Tribes and Hawaiian Natives Program: Review of Program Activities, 1990. Title IV, Library Services and Construction Act.

    ERIC Educational Resources Information Center

    Fine, Beth, Comp.; Woolen, Viola, Comp.

    THe Library Services for Indian Tribes and Hawaiian natives Program, Title IV of the Library Services and Construction Act (LSCA), supports public library services to Indians and Hawaiian Natives. Two types of grants--Basic Grants and Special Projects--encourage the development and improvement of public library services through selected project…

  20. Men's Adultery as Sickness: Metaphor, Gender Categories and the Discursive Construction of Gender Differences on Japanese Television Consultation Programs

    ERIC Educational Resources Information Center

    Saft, Scott; Ohara, Yumiko

    2009-01-01

    This article describes how Japanese TV programs adopting a consultation framework construct different realities for women and men concerning a specific social phenomenon, namely adultery. To do so, two perspectives, the theory of metaphor promoted by George Lakoff and membership category analysis, are combined toward a discursive analysis which…

  1. Massachusetts Long-Range Program Under Library Services and Construction Act (P.L. 91-600).

    ERIC Educational Resources Information Center

    Massachusetts State Dept. of Education, Boston. Bureau of Library Extension.

    As required under the Library Services and Construction Act, Massachusetts has developed a long-range program document delineating its goals, sub-goals, and objectives for library services. The overall goal for library service is to provide all residents of the Commonwealth access to those library and information resources which will satisfy their…

  2. When the School Bell Rings... Juvenile Crime or Constructive Time? After-School Programs Are the Answer.

    ERIC Educational Resources Information Center

    Carpenter, Tim; Cornelius, Aisha; Francis, Ann Potter; Parsons, Lena

    Noting that the after-school hours are peak hours for Illinois juveniles to be either victims of crime or involved in criminal activity, this report provides evidence that making quality after-school programs available to all youth who need them will reduce crime and provide constructive activities for youth. The report details statistics on…

  3. Lessons learned from new construction utility demand side management programs and their implications for implementing building energy codes

    SciTech Connect

    Wise, B.K.; Hughes, K.R.; Danko, S.L.; Gilbride, T.L.

    1994-07-01

    This report was prepared for the US Department of Energy (DOE) Office of Codes and Standards by the Pacific Northwest Laboratory (PNL) through its Building Energy Standards Program (BESP). The purpose of this task was to identify demand-side management (DSM) strategies for new construction that utilities have adopted or developed to promote energy-efficient design and construction. PNL conducted a survey of utilities and used the information gathered to extrapolate lessons learned and to identify evolving trends in utility new-construction DSM programs. The ultimate goal of the task is to identify opportunities where states might work collaboratively with utilities to promote the adoption, implementation, and enforcement of energy-efficient building energy codes.

  4. C1IV:. Gravitational Wave Data Analysis

    NASA Astrophysics Data System (ADS)

    Sathyaprakash, B. S.

    2002-09-01

    Resonant bar detectors are routinely searching for astronomical sources of gravitational waves and to setting upper limits on event rates. Interferometric detectors are beginning to operate at sensitivity levels good enough to set meaningful upper limits and begin astrophysical searches. With the long baseline interferometers scheduled to take data at unprecedented sensitivity levels the next few years will be a very exciting period for gravitational waves. In session C1iv there were talks focusing on gravitational wave searches (Krolak and Sintes), setting upper limits on astrophysical signals (Brady and Whelan), theoretical developments in modelling binary black holes (Iyer), testing general relativity with gravitational wave data (Will) and tools for gravitational wave data analysis (Schutz). There was also a one-hour round-table discussion on setting upper limits chaired by Andersson.

  5. Using a commercial CAD system for simultaneous input to theoretical aerodynamic programs and wind-tunnel model construction

    NASA Technical Reports Server (NTRS)

    Enomoto, F.; Keller, P.

    1984-01-01

    The Computer Aided Design (CAD) system's common geometry database was used to generate input for theoretical programs and numerically controlled (NC) tool paths for wind tunnel part fabrication. This eliminates the duplication of work in generating separate geometry databases for each type of analysis. Another advantage is that it reduces the uncertainty due to geometric differences when comparing theoretical aerodynamic data with wind tunnel data. The system was adapted to aerodynamic research by developing programs written in Design Analysis Language (DAL). These programs reduced the amount of time required to construct complex geometries and to generate input for theoretical programs. Certain shortcomings of the Design, Drafting, and Manufacturing (DDM) software limited the effectiveness of these programs and some of the Calma NC software. The complexity of aircraft configurations suggests that more types of surface and curve geometry should be added to the system. Some of these shortcomings may be eliminated as improved versions of DDM are made available.

  6. Improving safety climate through a communication and recognition program for construction: a mixed-methods study

    PubMed Central

    Sparer, Emily H; Catalano, Paul J; Herrick, Robert F; Dennerlein, Jack T

    2016-01-01

    Objectives This study aimed to evaluate the efficacy of a safety communication and recognition program (B-SAFE), designed to encourage improvement of physical working conditions and hazard reduction in construction. Methods A matched pair cluster randomized controlled trial was conducted on eight worksites (four received the B-SAFE intervention, four served as control sites) for approximately five months per site. Pre- and post-exposure worker surveys were collected at all sites (N=615, pre-exposure response rate of 74%, post-exposure response rate of 88%). Multi-level mixed-effect regression models evaluated the effect of B-SAFE on safety climate as assessed from surveys. Focus groups (N=6–8 workers/site) were conducted following data collection. Transcripts were coded and analyzed for thematic content using Atlas.ti (version 6). Results The mean safety climate score at intervention sites, as measured on a 0–50 point scale, increased 0.5 points (1%) between pre- and post-B-SAFE exposure, compared to control sites that decreased 0.8 points (1.6%). The intervention effect size was 1.64 (3.28%) (P-value=0.01) when adjusted for month the worker started on-site, total length of time on-site, as well as individual characteristics (trade, title, age, and race/ethnicity). At intervention sites, workers noted increased levels of safety awareness, communication, and teamwork compared to control sites. Conclusions B-SAFE led to many positive changes, including an improvement in safety climate, awareness, teambuilding, and communication. B-SAFE was a simple intervention that engaged workers through effective communication infrastructures and had a significant, positive effect on worksite safety. PMID:27158914

  7. Plasma levels of C1- inhibitor complexes and cleaved C1- inhibitor in patients with hereditary angioneurotic edema.

    PubMed

    Cugno, M; Nuijens, J; Hack, E; Eerenberg, A; Frangi, D; Agostoni, A; Cicardi, M

    1990-04-01

    C1- inhibitor (C1(-)-Inh) catabolism in plasma of patients with hereditary angioneurotic edema (HANE) was assessed by measuring the complexes formed by C1(-)-Inh with its target proteases (C1-s, Factor XIIa, and kallikrein) and a modified (cleaved) inactive form of C1(-)-Inh (iC1(-)-Inh). This study was performed in plasma from 18 healthy subjects and 30 patients with HANE in remission: 20 with low antigen concentration (type I) and 10 (from 5 different kindreds) with dysfunctional protein (type II). Both type-I and type-II patients had increased C1(-)-C1(-)-Inh complexes (P less than 0.0001), which in type I inversely correlated with the levels of C1(-)-Inh (P less than 0.001). iC1(-)-Inh was normal in all type-I patients and in type-II patients from three families with increased C1(-)-Inh antigen, whereas iC1(-)-Inh was higher than 20 times the normal values in patients from the remaining two families with C1(-)-Inh antigen in the normal range. None of the subjects had an increase of either Factor XIIa-C1(-)-Inh or kallikrein-C1(-)-Inh complexes. This study shows that the hypercatabolism of C1(-)-Inh in HANE patients at least in part occurs via the formation of complexes with C1- and that genetically determined differences in catabolism of dysfunctional C1(-)-Inh proteins are present in type-II patients.

  8. Libby Mitigation Program, 2007 Annual Progress Report: Mitigation for the Construction and Operation of Libby Dam.

    SciTech Connect

    Dunnigan, James; DeShazer, J.; Garrow, L.

    2009-05-26

    basin streams and lakes. 'Mitigation for the Construction and Operation of Libby Dam' is part of the Northwest Power and Conservation Council's (NPCC) resident fish and wildlife program. The program was mandated by the Northwest Planning Act of 1980, and is responsible for mitigating damages to fish and wildlife caused by hydroelectric development in the Columbia River Basin. The objective of Phase I of the project (1983 through 1987) was to maintain or enhance the Libby Reservoir fishery by quantifying seasonal water levels and developing ecologically sound operational guidelines. The objective of Phase II of the project (1988 through 1996) was to determine the biological effects of reservoir operations combined with biotic changes associated with an aging reservoir. The objectives of Phase III of the project (1996 through present) are to implement habitat enhancement measures to mitigate for dam effects, to provide data for implementation of operational strategies that benefit resident fish, monitor reservoir and river conditions, and monitor mitigation projects for effectiveness. This project completes urgent and high priority mitigation actions as directed by the Kootenai Subbasin Plan.

  9. Development of a Program Logic Model and Evaluation Plan for a Participatory Ergonomics Intervention in Construction

    PubMed Central

    Jaegers, Lisa; Dale, Ann Marie; Weaver, Nancy; Buchholz, Bryan; Welch, Laura; Evanoff, Bradley

    2013-01-01

    Background Intervention studies in participatory ergonomics (PE) are often difficult to interpret due to limited descriptions of program planning and evaluation. Methods In an ongoing PE program with floor layers, we developed a logic model to describe our program plan, and process and summative evaluations designed to describe the efficacy of the program. Results The logic model was a useful tool for describing the program elements and subsequent modifications. The process evaluation measured how well the program was delivered as intended, and revealed the need for program modifications. The summative evaluation provided early measures of the efficacy of the program as delivered. Conclusions Inadequate information on program delivery may lead to erroneous conclusions about intervention efficacy due to Type III error. A logic model guided the delivery and evaluation of our intervention and provides useful information to aid interpretation of results. PMID:24006097

  10. Structural and functional aspects of C1-inhibitor.

    PubMed

    Bos, Ineke G A; Hack, C Erik; Abrahams, Jan Pieter

    2002-09-01

    C1-Inh is a serpin that inhibits serine proteases from the complement and the coagulation pathway. C1-Inh consists of a serpin domain and a unique N-terminal domain and is heavily glycosylated. Non-functional mutants of C1-Inh can give insight into the inhibitory mechanism of C1-Inh. This review describes a novel 3D model of C1-Inh, based on a newly developed homology modelling method. This model gives insight into a possible potentiation mechanism of C1-Inh and based on this model the essential residues for efficient inhibition by C1-Inh are discussed.

  11. Evaluation of the Mandatory Construction Induction Training Program in Western Australia: Unanticipated Consequences

    ERIC Educational Resources Information Center

    Bahn, Susanne; Barratt-Pugh, Llandis

    2012-01-01

    Since January 1, 2007, Government legislation in Western Australia required all workers in construction to complete mandatory safety awareness training before they began work on site. During the implementation of this new legislation there was considerable resistance from the construction sector due to the mandatory nature of the training. The…

  12. Facility Planning in the Construction Grants Program. Instructor Guide. Working for Clean Water: An Information Program for Advisory Groups.

    ERIC Educational Resources Information Center

    Buskirk, E. Drannon, Jr.; Cole, Charles A.

    Wastewater facility planning is an essential component of the federal construction grants process. Presented in this instructor's guide is a one-hour presentation on facility planning intended for citizen advisory groups. The guide is part of the Working for Clean Water Project, which also includes a supplementary audiovisual presentation.…

  13. Characterization of C1 inhibitor binding to neutrophils.

    PubMed Central

    Chang, N S; Boackle, R J; Leu, R W

    1991-01-01

    In a previous study we have isolated neutrophil membrane proteins that non-covalently bind to native C1-INH (105,000 MW) and a non-functional, degraded C1-INH (88,000 MW; C1-INH-88). To further characterize the binding nature, we have designed a novel kinetic C1 titration assay which enables not only a quantification of the removal of fluid-phase C1-INH by neutrophils, but also a concomitant measure of residual C1-INH function. Native C1-INH, when adsorbed to EDTA-pretreated neutrophils, lost its function in the inhibition of fluid-phase C1. The non-functional C1-INH-88, which is probably devoid of a reactive centre, was found to block the binding of native C1-INH to neutrophils. Pretreatment of neutrophils with serine esterase inhibitors did not abrogate binding capacity of the cells for C1-INH, whereas the binding affinity for C1-INH was lost when the cells were pretreated with trypsin. An array of human peripheral blood leucocytes and several lymphoid cell lines has surface binding sites for C1-INH, but not on human erythrocytes and U937 cells. Binding was further confirmed using (i) C1-INH-microsphere beads to neutrophils, in which the binding was blocked when pretreating neutrophils with excess C1-INH or with trypsin, and (ii) radiolabelled C1-INH to neutrophils, which was competitively blocked by unlabelled non-functional C1-INH-88. Desialylation of C1-INH significantly reduced its binding affinity for neutrophils, indicating that the membrane receptor sites on neutrophils could be specific for the binding of sialic acid residues on C1-INH. Overall, our studies indicate that neutrophils or other leucocytes possess specific surface binding sites for the sialic acid-containing portion of C1-INH. PMID:2045131

  14. Structure and functions of the interaction domains of C1r and C1s: keystones of the architecture of the C1 complex.

    PubMed

    Thielens, N M; Bersch, B; Hernandez, J F; Arlaud, G J

    1999-05-01

    C1r and C1s, the proteases responsible for activation and proteolytic activity of the C1 complex of complement, share similar overall structural organizations featuring five nonenzymic protein modules (two CUB modules surrounding a single EGF module, and a pair of CCP modules) followed by a serine protease domain. Besides highly specific proteolytic activities, both proteases exhibit interaction properties associated with their N-terminal regions. These properties include the ability to bind Ca2+ ions with high affinity, to associate with each other within a Ca2+-dependent C1s-C1r-C1r-C1s tetramer, and to interact with C1q upon C1 assembly. Precise functional mapping of these regions has been achieved recently, allowing identification of the domains responsible for these interactions, and providing a comprehensive picture of their structure and function. The objective of this article is to provide a detailed and up-to-date overview of the information available on these domains, which are keystones of the assembly of C1, and appear to play an essential role at the interface between the recognition function of C1 and its proteolytic activity. PMID:10408360

  15. Creating, Constructing, and Cultivating Professional Development within a Reggio-Inspired Early Childhood Education Program

    ERIC Educational Resources Information Center

    Haigh, Karen M.

    2009-01-01

    The study examined the professional development system of an early childhood education program which was influenced by the Reggio Emilia Approach to early learning. This multi-site program thrived within low-income, inner-city communities of Chicago. Literature connected to the program's historical context of the Settlement House and the Reggio…

  16. 40 CFR 63.42 - Program requirements governing construction or reconstruction of major sources.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... construction or reconstruction of major sources. 63.42 Section 63.42 Protection of Environment ENVIRONMENTAL... POLLUTANTS FOR SOURCE CATEGORIES Requirements for Control Technology Determinations for Major Sources in... achievable control technology emission limitation for new sources....

  17. 11 CFR 9420.6 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... is constructed or altered by, on behalf of, or for the use of the Commission shall be designed..., requirements, and standards of the Architectural Barriers Act, 42 U.S.C. 4151-4157 apply to buildings...

  18. 49 CFR 1014.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR... that is constructed or altered by, on behalf of, or for the use of the agency shall be...

  19. An Evaluation of the Right Choices Program to Determine Effectiveness in Delivering Constructive Interventions and Providing an Early Support Program in Order to Modify Behavior of First-Time Student Offenders Who Commit Drug and Violent Acts

    ERIC Educational Resources Information Center

    Barnes, Lisa B.

    2010-01-01

    The purpose of the study was to perform a program evaluation of the Right Choices Program to determine the program's effectiveness in delivering constructive interventions that modify student behavior once students have left the program and have returned to their regular learning environment. This mixed-method evaluation consisted of an…

  20. Proteolytic inactivation of plasma C1- inhibitor in sepsis.

    PubMed

    Nuijens, J H; Eerenberg-Belmer, A J; Huijbregts, C C; Schreuder, W O; Felt-Bersma, R J; Abbink, J J; Thijs, L G; Hack, C E

    1989-08-01

    Activation of both the complement system and the contact system of intrinsic coagulation is implicated in the pathophysiology of sepsis. Because C1 inhibitor (C1-Inh) regulates the activation of both cascade systems, we studied the characteristics of plasma C1-Inh in 48 patients with severe sepsis on admission to the Intensive Care Unit at the Free University of Amsterdam. The ratio between the level of functional and antigenic C1-Inh (functional index) was significantly reduced in the patients with sepsis compared with healthy volunteers (P = 0.004). The assessment of modified (cleaved), inactive C1-Inh (iC1-Inh), and complexed forms of C1-Inh (nonfunctional C1-Inh species) revealed that the reduced functional index was mainly due to the presence of iC1-Inh. On SDS-PAGE, iC1-Inh species migrated with a lower apparent molecular weight (Mr 98,000, 91,000, and 86,000) than native C1-Inh (Mr 110,000). Elevated iC1-Inh levels (greater than or equal to 0.13 microM) were found in 81% of all patients, sometimes up to 1.6 microM. Levels of iC1-Inh on admission appeared to be of prognostic value: iC1-Inh was higher in 27 patients who died than in 21 patients who survived (P = 0.003). The mortality in 15 patients with iC1-Inh levels up to 0.2 microM was 27%, but in 12 patients with plasma iC1-Inh exceeding 0.44 microM, the mortality was 83%. The overall mortality in the patients with sepsis was 56%. We propose that the cleavage of C1-Inh in patients with sepsis reflects processes that play a major role in the development of fatal complications during sepsis.

  1. 40 CFR 63.42 - Program requirements governing construction or reconstruction of major sources.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR... Accordance With Clean Air Act Sections, Sections 112(g) and 112(j) § 63.42 Program requirements governing... cause by EPA to apply the sanctions and remedies set forth in the Clean Air Act section 502(I)....

  2. 40 CFR 63.42 - Program requirements governing construction or reconstruction of major sources.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR... Accordance With Clean Air Act Sections, Sections 112(g) and 112(j) § 63.42 Program requirements governing... cause by EPA to apply the sanctions and remedies set forth in the Clean Air Act section 502(I)....

  3. 40 CFR 63.42 - Program requirements governing construction or reconstruction of major sources.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR... Accordance With Clean Air Act Sections, Sections 112(g) and 112(j) § 63.42 Program requirements governing... cause by EPA to apply the sanctions and remedies set forth in the Clean Air Act section 502(I)....

  4. 40 CFR 63.42 - Program requirements governing construction or reconstruction of major sources.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR... Accordance With Clean Air Act Sections, Sections 112(g) and 112(j) § 63.42 Program requirements governing... cause by EPA to apply the sanctions and remedies set forth in the Clean Air Act section 502(I)....

  5. 43 CFR 17.551 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ..., requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157) as established in 41 CFR... NONDISCRIMINATION IN FEDERALLY ASSISTED PROGRAMS OF THE DEPARTMENT OF THE INTERIOR Enforcement of Nondiscrimination on the Basis of Handicap in Programs or Activities Conducted by the Department of the Interior §...

  6. 43 CFR 17.551 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ..., requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157) as established in 41 CFR... NONDISCRIMINATION IN FEDERALLY ASSISTED PROGRAMS OF THE DEPARTMENT OF THE INTERIOR Enforcement of Nondiscrimination on the Basis of Handicap in Programs or Activities Conducted by the Department of the Interior §...

  7. 43 CFR 17.551 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ..., requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157) as established in 41 CFR... NONDISCRIMINATION IN FEDERALLY ASSISTED PROGRAMS OF THE DEPARTMENT OF THE INTERIOR Enforcement of Nondiscrimination on the Basis of Handicap in Programs or Activities Conducted by the Department of the Interior §...

  8. Indigenous Worldviews in Intercultural Education: Teachers' Construction of Interculturalism in a Bilingual Quechua-Spanish Program

    ERIC Educational Resources Information Center

    Valdiviezo, Laura Alicia

    2010-01-01

    This paper examines teachers' implementation of a bilingual intercultural education (BIE) program in Peru. This program is inspired by global policies that promote cultural pluralism and educational access to marginalized indigenous populations. Broadly addressed in policy in Andean countries, interculturalism in Peru has remained a core…

  9. Studies directed toward the total synthesis of discodermolide: asymmetric synthesis of the C1-C14 fragment.

    PubMed

    Arefolov, Alexander; Panek, James S

    2002-07-11

    [structure: see text] A convergent and stereoselective assembly of the C1-C14 subunit of marine natural product (+)-discodermolide has been completed. The approach employs chiral allylsilane bond construction methodology to establish four of the eight stereogenic centers. Key fragment coupling is achieved via an efficient stereoselective acetate aldol reaction between C1-C6 and C7-C14 subunits.

  10. The Effects of "Girl-Friendly" Schools: Evidence from the BRIGHT School Construction Program in Burkina Faso. NBER Working Paper No. 18115

    ERIC Educational Resources Information Center

    Kazianga, Harounan; Levy, Dan; Linden, Leigh L.; Sloan, Matt

    2012-01-01

    We evaluate the causal effects of a program that constructed high quality "girl-friendly" primary schools in Burkina Faso, using a regression discontinuity design 2.5 years after the program started. We find that the program increased enrollment of all children between the ages of 5 and 12 by 20 percentage points and increased their test scores by…

  11. Mid-Atlantic Regional Training Center for Residential Construction Trades. Final Program Evaluation Report.

    ERIC Educational Resources Information Center

    Wasdyke (R. G.) & Associates, Annapolis, MD.

    A group of partners headed by the Home Builders Institute (HBI) created the Mid-Atlantic Regional Training (MART) Center for Residential Construction, with a primary focus on providing education and training services related to the masonry and carpentry trades at existing institutions in the District of Columbia, Maryland, Virginia, and West…

  12. 48 CFR 252.225-7045 - Balance of Payments Program-Construction Material Under Trade Agreements.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 3 2011-10-01 2011-10-01 false Balance of Payments... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions And Clauses 252.225-7045 Balance of Payments... clause: Balance of Payments Program—Construction Material Under Trade Agreements (JUN 2011)...

  13. 48 CFR 252.225-7045 - Balance of Payments Program-Construction Material Under Trade Agreements.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 3 2012-10-01 2012-10-01 false Balance of Payments... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions And Clauses 252.225-7045 Balance of Payments... clause: Balance of Payments Program—Construction Material Under Trade Agreements (MAY 2012)...

  14. 48 CFR 252.225-7044 - Balance of Payments Program-Construction Material.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 3 2011-10-01 2011-10-01 false Balance of Payments... AND CONTRACT CLAUSES Text of Provisions And Clauses 252.225-7044 Balance of Payments Program—Construction Material. As prescribed in 225.7503(a)(1), use the following clause: Balance of Payments...

  15. 48 CFR 252.225-7045 - Balance of Payments Program-Construction Material Under Trade Agreements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Balance of Payments... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions And Clauses 252.225-7045 Balance of Payments... clause: Balance of Payments Program—Construction Material Under Trade Agreements (NOV 2009)...

  16. 48 CFR 252.225-7044 - Balance of Payments Program-Construction Material.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 3 2014-10-01 2014-10-01 false Balance of Payments... AND CONTRACT CLAUSES Text of Provisions And Clauses 252.225-7044 Balance of Payments Program—Construction Material. As prescribed in 225.7503(a)(1), use the following clause: Balance of Payments...

  17. 48 CFR 252.225-7045 - Balance of Payments Program-Construction Material Under Trade Agreements.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 3 2013-10-01 2013-10-01 false Balance of Payments... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions And Clauses 252.225-7045 Balance of Payments... clause: Balance of Payments Program—Construction Material Under Trade Agreements (AUG 2013)...

  18. 48 CFR 252.225-7044 - Balance of Payments Program-Construction Material.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 3 2012-10-01 2012-10-01 false Balance of Payments... AND CONTRACT CLAUSES Text of Provisions And Clauses 252.225-7044 Balance of Payments Program—Construction Material. As prescribed in 225.7503(a)(1), use the following clause: Balance of Payments...

  19. 48 CFR 252.225-7044 - Balance of Payments Program-Construction Material.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 3 2013-10-01 2013-10-01 false Balance of Payments... AND CONTRACT CLAUSES Text of Provisions And Clauses 252.225-7044 Balance of Payments Program—Construction Material. As prescribed in 225.7503(a)(1), use the following clause: Balance of Payments...

  20. 48 CFR 252.225-7045 - Balance of Payments Program-Construction Material Under Trade Agreements.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 3 2014-10-01 2014-10-01 false Balance of Payments... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions And Clauses 252.225-7045 Balance of Payments... clause: Balance of Payments Program—Construction Material Under Trade Agreements (OCT 2013)...

  1. Facility Programming and Construction Criteria [Planning Guide]. 702 KAR 4:170.

    ERIC Educational Resources Information Center

    Kentucky State Dept. of Education, Frankfort. Div. of Facilities Management.

    This facility construction planning guide presents the minimum instructional space standards for Kentucky's public school system. It provides definitions of terms found in the regulations; presents space requirements for every type of instructional space within a public school, including circulation areas, storage, and mechanical/electrical areas;…

  2. Predators of Knowledge Construction: Interpreting Students' Metacognition in an Amusement Park Physics Program

    ERIC Educational Resources Information Center

    Anderson, David; Nashon, Samson

    2007-01-01

    It is recognized widely that learning is a dynamic and idiosyncratic process of construction and reconstruction of concepts in response to new experiences. It is influenced by the learner's prior knowledge, motivation, and sociocultural context. This study investigated how year 11 and 12 physics students' metacognition influences the development…

  3. Task Inventory Construction. Evaluation of the Marine Corps Task Analysis Program. Technical Report No. 14.

    ERIC Educational Resources Information Center

    Kishi, Akemi

    To aid in the construction of effective task analysis inventories, this technical report discusses: (1) an optimum questionnaire length that adequately covers Marine tasks without unduly fatiguing respondents; (2) procedures for the phrasing of task statements to avoid ambiguities and be understandable to as broad a range of Marines as is…

  4. Template Construction as a Basis for Error-Analysis Packages in Language Learning Programs.

    ERIC Educational Resources Information Center

    Helmreich, Stephen C.

    1987-01-01

    An "intelligent" system for constructing computer-assisted pattern drills to be used in second language instruction is proposed. First, some of the difficulties in designing intelligent error analysis are discussed briefly. Two major approaches to error analysis in computer-assisted instruction, pattern matching and parsing, are described, and…

  5. 45 CFR 1175.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...) NATIONAL FOUNDATION ON THE ARTS AND THE HUMANITIES NATIONAL ENDOWMENT FOR THE HUMANITIES ENFORCEMENT OF... building that is constructed or altered by, on behalf of, or for the use of the agency shall be designed... 41 CFR 101-19.600 to 101-19.607, apply to buildings covered by this section....

  6. 45 CFR 85.43 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... agency shall be designed, constructed, or altered so as to be readily accessible to and usable by individuals with handicaps. The definitions, requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157) as established in 41 CFR 101-19.600 to 101-19.607 apply to buildings covered...

  7. 41 CFR 51-10.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to... altered by, on behalf of, or for the use of the agency shall be designed, constructed, or altered so as...

  8. 29 CFR 2205.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to 101-19... by, on behalf of, or for the use of the agency shall be designed, constructed, or altered so as to...

  9. 16 CFR 1034.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... agency shall be designed, constructed, or altered so as to be readily accessible to and usable by handicapped persons. The definitions, requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to 101-19.607, apply to buildings covered by...

  10. 5 CFR 2416.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to 101-19.607, apply to... use of the agency shall be designed, constructed, or altered so as to be readily accessible to...

  11. 17 CFR 200.651 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... building that is constructed or altered by, on behalf of, or for the use of the agency shall be designed... definitions, requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to 101-19.607, apply to buildings covered by this section....

  12. 36 CFR 909.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to 101-19.607, apply to... of, or for the use of the agency shall be designed, constructed, or altered so as to be...

  13. 31 CFR 17.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... agency shall be designed, constructed, or altered so as to be readily accessible to and usable by individuals with handicaps. The definitions, requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 through 101-19.607 apply to buildings...

  14. 29 CFR 2706.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to 101-19... by, on behalf of, or for the use of the agency shall be designed, constructed, or altered so as to...

  15. 5 CFR 1636.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to 101-19.607, apply to... of, or for the use of the agency shall be designed, constructed, or altered so as to be...

  16. 36 CFR 1154.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to... construction and alterations. 1154.151 Section 1154.151 Parks, Forests, and Public Property ARCHITECTURAL AND... ACTIVITIES CONDUCTED BY THE ARCHITECTURAL AND TRANSPORTATION BARRIERS COMPLIANCE BOARD § 1154.151...

  17. 13 CFR 136.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... the Agency shall be designed, constructed, or altered so as to be readily accessible to and usable by individuals with handicaps. The definitions, requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600—101-19.607, apply to buildings covered...

  18. 36 CFR 1208.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to... altered by, on behalf of, or for the use of the agency shall be designed, constructed, or altered so as...

  19. 34 CFR 1200.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to... by, on behalf of, or for the use of the agency shall be designed, constructed, or altered so as to...

  20. 36 CFR 406.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... use of the agency shall be designed, constructed, or altered so as to be readily accessible to and usable by handicapped persons. The definitions, requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to 101-19.607, apply to...

  1. 38 CFR 15.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to 101-19.607, apply to..., or for the use of the agency shall be designed, constructed, or altered so as to be...

  2. 36 CFR 812.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to 101-19.607, apply to..., or for the use of the agency shall be designed, constructed, or altered so as to be...

  3. 3 CFR 102.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... building that is constructed or altered by, on behalf of, or for the use of the agency shall be designed... definitions, requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to 101-19.607, apply to buildings covered by this section....

  4. 14 CFR 1251.551 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to... by, on behalf of, or for the use of the agency shall be designed, constructed, or altered so as to...

  5. 40 CFR 12.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... shall be designed, constructed, or altered so as to be readily accessible to and usable by individuals with handicaps. The definitions, requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to 101-19.607, apply to buildings covered by...

  6. 5 CFR 1850.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... designed, constructed, or altered so as to be readily accessible to and usable by individuals with handicaps. The definitions, requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to 101-19.607, apply to buildings covered by this section....

  7. 44 CFR 16.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to... altered by, on behalf of, or for the use of the agency shall be designed, constructed, or altered so as...

  8. A Concept of Constructing a Common Information Space for High Tech Programs Using Information Analytical Systems

    NASA Astrophysics Data System (ADS)

    Zakharova, Alexandra A.; Kolegova, Olga A.; Nekrasova, Maria E.

    2016-04-01

    The paper deals with the issues in program management used for engineering innovative products. The existing project management tools were analyzed. The aim is to develop a decision support system that takes into account the features of program management used for high-tech products: research intensity, a high level of technical risks, unpredictable results due to the impact of various external factors, availability of several implementing agencies. The need for involving experts and using intelligent techniques for information processing is demonstrated. A conceptual model of common information space to support communication between members of the collaboration on high-tech programs has been developed. The structure and objectives of the information analysis system “Geokhod” were formulated with the purpose to implement the conceptual model of common information space in the program “Development and production of new class mining equipment – “Geokhod”.

  9. A Concept of Constructing a Common Information Space for High Tech Programs Using Information Analytical Systems

    NASA Astrophysics Data System (ADS)

    Zakharova, Alexandra A.; Kolegova, Olga A.; Nekrasova, Maria E.

    2016-04-01

    The paper deals with the issues in program management used for engineering innovative products. The existing project management tools were analyzed. The aim is to develop a decision support system that takes into account the features of program management used for high-tech products: research intensity, a high level of technical risks, unpredictable results due to the impact of various external factors, availability of several implementing agencies. The need for involving experts and using intelligent techniques for information processing is demonstrated. A conceptual model of common information space to support communication between members of the collaboration on high-tech programs has been developed. The structure and objectives of the information analysis system “Geokhod” were formulated with the purpose to implement the conceptual model of common information space in the program “Development and production of new class mining equipment - “Geokhod”.

  10. Low molecular weight C1q in systemic lupus erythematosus.

    PubMed

    Hoekzema, R; Hannema, A J; Swaak, T J; Paardekooper, J; Hack, C E

    1985-07-01

    In sera of patients suffering from an exacerbation of systemic lupus erythematosus (SLE), increased amounts of abnormal C1q were detected, contrasting with decreased or even undetectable levels of normal C1q in these sera. When analyzed immunochemically by double immunodiffusion, this low m.w. C1q (LMW-C1q) appeared to be identical with the defective C1q in serum of individuals with an inherited, homozygous inability to produce functional plasma C1q. These persons show a tendency to develop SLE-like syndromes. Like the genetically defective C1q, the abnormal C1q molecule in SLE sera was hemolytically inactive, did not incorporate in C1, was found in the supernatant of euglobulin-precipitated serum, and appeared in the break-through fraction of a cation-exchange column. Sucrose gradients and gel filtration analyses supported the putative identity of the molecules. SDS-PAGE and immunoblots revealed the presence of subunits that reacted with antibodies against C1q and confirmed the C1q-like nature of LMW-C1q. Low levels of LMW-C1q were also detected in serum and plasma of normal individuals. A radial immunodiffusion technique was used to measure LMW-C1q in the serum of 54 patients. Although these patients were not selected for parameters of disease activity, their levels of LMW-C1q were significantly higher than those of normal individuals and children with decreased C3 levels due to acute glomerulonephritis.

  11. Automatic planning and programming for robotic construction of planetary/lunar structures

    SciTech Connect

    Boissiere, P.T.

    1997-04-01

    Many difficult material handling needs exist in remote unstructured environments. Currently these operations are accomplished using personnel in direct contact with the hazards. A safe and cost-effective alternative to this approach is the use of intelligent robotic systems for the excavation, handling, transport and manipulation of materials during remote construction operations. A robotic system designed for these tasks has been developed at Sandia National Laboratories which successfully integrates computer controlled manipulation and mobility to deliver these needed robotic capabilities in remote applications such as planetary operations. The mobile robot, RETRVIR, incorporates advanced developments in the integration of sensors, advanced computing environments, and graphical user interfaces. The addition of these elements in the control of remotely operated equipment have shown great promise for reducing the cost of remote construction while providing faster and safer operations.

  12. Construction of an optimal background profile for the Kuramoto-Sivashinsky equation using semidefinite programming

    NASA Astrophysics Data System (ADS)

    Fantuzzi, G.; Wynn, A.

    2015-01-01

    A method to construct systematically an optimal background profile for the Kuramoto-Sivashinsky equation is developed by formulating the classical problem as an optimisation problem. In particular, we show that the infinite-dimensional problem can be rewritten as a finite-dimensional convex semidefinite problem, which is solved to construct a background profile and to obtain an upper bound on the energy of the solution ‖ u ‖ that applies to the infinite-dimensional PDE. The results are compared to existing analytical results, and support the fact that lim sup t → ∞ ‖ u ‖ ≤ O (L 3 / 2) is the optimal estimate achievable with the background profile method and a quadratic Lyapunov function.

  13. Recombinant human C1-inhibitor produced in Pichia pastoris has the same inhibitory capacity as plasma C1-inhibitor.

    PubMed

    Bos, Ineke G A; de Bruin, Eric C; Karuntu, Yani A; Modderman, Piet W; Eldering, Eric; Hack, C Erik

    2003-05-30

    Therapeutic application of the serpin C1-inhibitor (C1-Inh) in inflammatory diseases like sepsis, acute myocardial infarction and vascular leakage syndrome seems promising, but large doses may be required. Therefore, a high-yield recombinant expression system for C1-Inh is very interesting. Earlier attempts to produce high levels of C1-Inh resulted in predominantly inactive C1-Inh. We describe the high yield expression of rhC1-Inh in Pichia pastoris, with 180 mg/l active C1-Inh at maximum. On average, 30 mg/l of 80-100% active C1-Inh was obtained. Progress curves were used to study the interaction with C1s, kallikrein, coagulation factor XIIa and XIa, and demonstrated that rhC1-Inh had the same inhibitory capacity as plasma C1-Inh. Structural integrity, as monitored via heat stability, was comparable despite differences in extent and nature of glycosylation. We conclude that the P. pastoris system is capable of high-level production of functionally and structurally intact human C1 inhibitor.

  14. Program fullerene: a software package for constructing and analyzing structures of regular fullerenes.

    PubMed

    Schwerdtfeger, Peter; Wirz, Lukas; Avery, James

    2013-06-30

    Fullerene (Version 4.4) is a general purpose open-source program that can generate any fullerene isomer, perform topological and graph theoretical analysis, as well as calculate a number of physical and chemical properties. The program creates symmetric planar drawings of the fullerene graph and generates accurate molecular 3D geometries by way of force-field optimization, serving as a good starting point for further quantum theoretical treatments. It includes a number of fullerene-to-fullerene transformations, such as Goldberg-Coxeter transforms, Stone-Wales transforms, Endo-Kroto, Yoshida-Fowler, and Brinkmann-Fowler vertex insertions. The program is written in standard Fortran and C++ and can easily be installed in a Linux or UNIX environment. PMID:23559399

  15. Complement C1-inhibitor expression in Alzheimer's disease.

    PubMed

    Veerhuis, R; Janssen, I; Hoozemans, J J; De Groot, C J; Hack, C E; Eikelenboom, P

    1998-09-01

    In situ and in vitro studies suggest that activation of locally produced complement factors may act as a mediator between amyloid deposits and neurodegenerative changes seen in Alzheimer's disease (AD). C1-esterase inhibitor (C1-Inh), which regulates activation of C1 of the complement classical pathway, can be detected immunohistochemically in its inactivated form in activated astrocytes and dystrophic neurites in AD plaque areas. In this study, designed to investigate the cellular source of C1-Inh, C1-Inh was found to be secreted in a functionally active form by astrocytes cultured from postmortem human brain specimens as well as by neuroblastoma cell lines. Recombinant human interferon-gamma (IFN-gamma), which stimulates C1-Inh synthesis in various cell types, several-fold stimulated C1-Inh protein secretion by cultured human astrocytes derived from different regions of the central nervous system and by one (SK-N-SH) of two neuroblastoma cell lines (SK-N-SH and IMR-32) included in this study. In contrast to IFN-gamma, other cytokines [interleukin (IL)-1beta, IL-6 and tumor necrosis factor (TNF)-alpha] that can be found in brain areas affected by AD, did not stimulate C1-Inh secretion by astrocytes or neuroblastomas in vitro. This inability to secrete C1-Inh is probably due to unresponsiveness at the transcriptional level, since C1-Inh secretion paralleled the expression of the 2.1-kb C1-Inh mRNA. In situ hybridization with a C1-Inh RNA antisense probe labeled neurons rather than astrocytes, suggesting a role for neurons as producers of complement regulatory proteins in vivo. Since IFN-gamma is apparently lacking in the brain parenchyma, and amyloid plaque-associated cytokines (IL-1beta, IL-6, TNF-alpha) do not stimulate C1-Inh expression in vitro, the nature of the stimulus responsible for neuronal C1-Inh expression in AD brains remains to be investigated.

  16. 50 CFR 550.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ..., requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR... and alterations. 550.151 Section 550.151 Wildlife and Fisheries MARINE MAMMAL COMMISSION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY MARINE...

  17. 50 CFR 550.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ..., requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR... and alterations. 550.151 Section 550.151 Wildlife and Fisheries MARINE MAMMAL COMMISSION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY MARINE...

  18. 50 CFR 550.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ..., requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR... and alterations. 550.151 Section 550.151 Wildlife and Fisheries MARINE MAMMAL COMMISSION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY MARINE...

  19. 45 CFR 1706.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ..., requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR...) NATIONAL COMMISSION ON LIBRARIES AND INFORMATION SCIENCE ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY NATIONAL COMMISSION ON LIBRARIES AND INFORMATION...

  20. 50 CFR 550.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ..., requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR... and alterations. 550.151 Section 550.151 Wildlife and Fisheries MARINE MAMMAL COMMISSION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY MARINE...

  1. 50 CFR 550.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR... and alterations. 550.151 Section 550.151 Wildlife and Fisheries MARINE MAMMAL COMMISSION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY MARINE...

  2. 29 CFR 2205.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to 101-19.... 2205.151 Section 2205.151 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND... ACTIVITIES CONDUCTED BY THE OCCUPATIONAL SAFETY AND HEALTH REVIEW COMMISSION § 2205.151 Program...

  3. An Exploratory Study of the Impact of College Student Leadership Programs upon the Construct of Mindfulness

    ERIC Educational Resources Information Center

    Horowitz, Rich

    2012-01-01

    Mindfulness is a key skill that produces the greater psychological awareness identified by most leadership scholars as being essential to effective leadership. This study conducted an exploratory assessment of the effectiveness of mindfulness-based cocurricular collegiate leadership programs through pre- and post-participation application of the…

  4. 19 CFR 201.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... alterations. 201.151 Section 201.151 Customs Duties UNITED STATES INTERNATIONAL TRADE COMMISSION GENERAL RULES... Activities Conducted by the U.S. International Trade Commission § 201.151 Program accessibility: New... Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to 101-19.607, apply...

  5. 19 CFR 201.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... alterations. 201.151 Section 201.151 Customs Duties UNITED STATES INTERNATIONAL TRADE COMMISSION GENERAL RULES... Activities Conducted by the U.S. International Trade Commission § 201.151 Program accessibility: New... Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to 101-19.607, apply...

  6. Chart Your Own Course: Class Difference and the Construction of Personal Trajectories in Career Portfolio Programs

    ERIC Educational Resources Information Center

    Collin, Ross

    2011-01-01

    In this article, I examine two students' experiences with their schools' career portfolio programs. These initiatives call students to assemble papers, projects, and nonacademic items and to present them in ways that chart their personal trajectories into paid work. Shortly before graduation, students present their portfolios to exit interview…

  7. 29 CFR 2205.151 - Program accessibility: new construction and alterations.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    .... 2205.151 Section 2205.151 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH REVIEW COMMISSION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF DISABILITY IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE OCCUPATIONAL SAFETY AND HEALTH REVIEW COMMISSION AND IN ACCESSIBILITY...

  8. 29 CFR 2205.151 - Program accessibility: new construction and alterations.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    .... 2205.151 Section 2205.151 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH REVIEW COMMISSION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF DISABILITY IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE OCCUPATIONAL SAFETY AND HEALTH REVIEW COMMISSION AND IN ACCESSIBILITY...

  9. 29 CFR 2205.151 - Program accessibility: new construction and alterations.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    .... 2205.151 Section 2205.151 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH REVIEW COMMISSION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF DISABILITY IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE OCCUPATIONAL SAFETY AND HEALTH REVIEW COMMISSION AND IN ACCESSIBILITY...

  10. 22 CFR 530.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR... alterations. 530.151 Section 530.151 Foreign Relations BROADCASTING BOARD OF GOVERNORS ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE BROADCASTING BOARD...

  11. 22 CFR 530.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR... alterations. 530.151 Section 530.151 Foreign Relations BROADCASTING BOARD OF GOVERNORS ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE BROADCASTING BOARD...

  12. 22 CFR 530.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR... alterations. 530.151 Section 530.151 Foreign Relations BROADCASTING BOARD OF GOVERNORS ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE BROADCASTING BOARD...

  13. 22 CFR 530.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR... alterations. 530.151 Section 530.151 Foreign Relations BROADCASTING BOARD OF GOVERNORS ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE BROADCASTING BOARD...

  14. 22 CFR 530.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR... alterations. 530.151 Section 530.151 Foreign Relations BROADCASTING BOARD OF GOVERNORS ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE BROADCASTING BOARD...

  15. Application of Matrix Outcome Mapping to Constructively Align Program Outcomes and Course Outcomes in Higher Education

    ERIC Educational Resources Information Center

    Mazouz, Abdelkader; Crane, Keenan

    2013-01-01

    Establishing a link between Course Learning Outcomes (LOs) and Program Outcomes (POs) while assessing the course contents and delivery are among the most challenging issues in Higher Education. In the present study two forms were generated based on specific Course Learning Outcomes identified in the syllabus at the beginning of the teaching term:…

  16. 22 CFR 1600.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    .... 4151-4157), as established in 41 CFR 101-19.600 to 101-19.607, apply to buildings covered by this... alterations. 1600.151 Section 1600.151 Foreign Relations JAPAN-UNITED STATES FRIENDSHIP COMMISSION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE...

  17. Green Construction and Energy Training Program for At-Risk Individuals: A Case Study

    ERIC Educational Resources Information Center

    del Puerto, Carla Lopez; Crowson, Adrienne

    2013-01-01

    Training individuals who are at risk of unemployment/underemployment to increase their employability is a mission of many nonprofit agencies. These training programs, often supported by government funding, attempt to reduce these individuals' reliance on government assistance. The purpose of this study is to obtain hard data and an in-depth…

  18. 22 CFR 1005.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR... alterations. 1005.151 Section 1005.151 Foreign Relations INTER-AMERICAN FOUNDATION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE INTER-AMERICAN...

  19. 22 CFR 1005.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR... alterations. 1005.151 Section 1005.151 Foreign Relations INTER-AMERICAN FOUNDATION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE INTER-AMERICAN...

  20. 22 CFR 1005.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR... alterations. 1005.151 Section 1005.151 Foreign Relations INTER-AMERICAN FOUNDATION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE INTER-AMERICAN...

  1. 22 CFR 1005.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR... alterations. 1005.151 Section 1005.151 Foreign Relations INTER-AMERICAN FOUNDATION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE INTER-AMERICAN...

  2. 45 CFR 2490.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to... MADISON MEMORIAL FELLOWSHIP FOUNDATION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE JAMES MADISON MEMORIAL FELLOWSHIP FOUNDATION § 2490.151...

  3. 22 CFR 1510.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... established in 41 CFR 101-19.600 to 101-19.607, apply to buildings covered by this section. ... alterations. 1510.151 Section 1510.151 Foreign Relations AFRICAN DEVELOPMENT FOUNDATION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE AFRICAN DEVELOPMENT...

  4. 22 CFR 1510.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... established in 41 CFR 101-19.600 to 101-19.607, apply to buildings covered by this section. ... alterations. 1510.151 Section 1510.151 Foreign Relations AFRICAN DEVELOPMENT FOUNDATION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE AFRICAN DEVELOPMENT...

  5. 22 CFR 1510.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... established in 41 CFR 101-19.600 to 101-19.607, apply to buildings covered by this section. ... alterations. 1510.151 Section 1510.151 Foreign Relations AFRICAN DEVELOPMENT FOUNDATION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE AFRICAN DEVELOPMENT...

  6. 45 CFR 2490.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to... MADISON MEMORIAL FELLOWSHIP FOUNDATION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE JAMES MADISON MEMORIAL FELLOWSHIP FOUNDATION § 2490.151...

  7. 45 CFR 2490.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to... MADISON MEMORIAL FELLOWSHIP FOUNDATION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE JAMES MADISON MEMORIAL FELLOWSHIP FOUNDATION § 2490.151...

  8. 45 CFR 2490.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to... MADISON MEMORIAL FELLOWSHIP FOUNDATION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE JAMES MADISON MEMORIAL FELLOWSHIP FOUNDATION § 2490.151...

  9. 22 CFR 1510.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... established in 41 CFR 101-19.600 to 101-19.607, apply to buildings covered by this section. ... alterations. 1510.151 Section 1510.151 Foreign Relations AFRICAN DEVELOPMENT FOUNDATION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE AFRICAN DEVELOPMENT...

  10. 22 CFR 1005.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR... alterations. 1005.151 Section 1005.151 Foreign Relations INTER-AMERICAN FOUNDATION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE INTER-AMERICAN...

  11. 22 CFR 1510.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... established in 41 CFR 101-19.600 to 101-19.607, apply to buildings covered by this section. ... alterations. 1510.151 Section 1510.151 Foreign Relations AFRICAN DEVELOPMENT FOUNDATION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE AFRICAN DEVELOPMENT...

  12. 45 CFR 2490.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41 CFR 101-19.600 to... MADISON MEMORIAL FELLOWSHIP FOUNDATION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE JAMES MADISON MEMORIAL FELLOWSHIP FOUNDATION § 2490.151...

  13. Emergent Entities and Emergent Processes: Constructing Emergence through Multi-Agent Programming.

    ERIC Educational Resources Information Center

    Wilensky, Uri

    The use of programming language was studied in an eighth grade science classroom. The goal of the EMERGE curriculum is to enable students to understand the global patterns that they observe. EMERGE was used in an eighth-grade classroom of 12 students, with a range of abilities (3 had learning disabilities), in 21 sessions over 3 months. For the…

  14. 45 CFR 1706.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...) NATIONAL COMMISSION ON LIBRARIES AND INFORMATION SCIENCE ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY NATIONAL COMMISSION ON LIBRARIES AND INFORMATION SCIENCE..., requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41...

  15. 45 CFR 1706.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...) NATIONAL COMMISSION ON LIBRARIES AND INFORMATION SCIENCE ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY NATIONAL COMMISSION ON LIBRARIES AND INFORMATION SCIENCE..., requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41...

  16. 2005 Mississippi Curriculum Framework: Secondary Building Trades. (Program CIP: 46.0000 - Construction Trades, General)

    ERIC Educational Resources Information Center

    Jernigan, Jarvis; Manning, Phillip; Matkins, Billy

    2005-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  17. 45 CFR 1706.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ...) NATIONAL COMMISSION ON LIBRARIES AND INFORMATION SCIENCE ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY NATIONAL COMMISSION ON LIBRARIES AND INFORMATION SCIENCE..., requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41...

  18. 45 CFR 1706.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ...) NATIONAL COMMISSION ON LIBRARIES AND INFORMATION SCIENCE ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY NATIONAL COMMISSION ON LIBRARIES AND INFORMATION SCIENCE..., requirements, and standards of the Architectural Barriers Act (42 U.S.C. 4151-4157), as established in 41...

  19. 45 CFR 1181.151 - Program accessibility: New construction and alterations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... established in 41 CFR 101-19.600 to 101-19.607, apply to buildings covered by this section. ...) NATIONAL FOUNDATION ON THE ARTS AND THE HUMANITIES INSTITUTE OF MUSEUM AND LIBRARY SERVICES ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE INSTITUTE OF MUSEUM...

  20. Construction safety program for the National Ignition Facility Appendix A: Safety Requirements

    SciTech Connect

    Cerruti, S.J.

    1997-01-14

    These rules apply to all LLNL employees, non-LLNL employees (including contract labor, supplemental labor, vendors, personnel matrixed/assigned from other National Laboratories, participating guests, visitors and students) and construction contractors/subcontractors. The General Safety and Health rules shall be used by management to promote accident prevention through indoctrination, safety and health training and on-the-job application. As a condition for contracts award, all contractors and subcontractors and their employees must certify on Form S & H A-1 that they have read and understand, or have been briefed and understand, the National Ignition Facility OCIP Project General Safety Rules.

  1. Recent advances in the EPRI high-concentration photovoltaic program. Volume 2: Module and array programs; design, construction, and testing; system and open issues: Interim report

    SciTech Connect

    McNaughton, W.P.; Richman, R.H.

    1992-02-01

    By the early 1980s, Stanford University under contract with the Electric Power Research Institute (EPRI) produced a point-contact photovoltaic cell that has established a number of world record achievements. In 1984 a conceptual design was proposed for a cell package, module, and array system for use in utility-scale commercial power production. Since 1984, EPRI has moved the laboratory concept forward toward commercial application. More than 1500 high concentration cells have been produced in the evolutionary process. Approximately 700 of those cells have been or are presently being installed in fully operational modules for field tests. Over 130 modules have been constructed and are in field testing for electrical, thermal or weathering tests. Two full-sized array structures (each containing 60 instrumented ``thermal`` modules) have been constructed and continue to undergo field testing for alignment, performance, and reliability. This two volume report reviews the key challenges that have confronted the development of a commercial system and describes the program response to those challenges. The intent is to inform interested readers -- utilities, commercializers, researchers -- as to the primary developments and test results that have been achieved. A considerable body of ``raw data`` has been included so that interested researchers can draw their own conclusions without needing to refer to the source documents. Volume 1 discusses design fabrication and performance of the cell; design, construction and testing of the cell package and the design, fabrication and testing of the optical system. Volume 2 reviews the module and array programs, discusses other system issues and presents a short review of the technology`s open issues.

  2. [Constructing an ACP Simulation-Situation Communication Training Program for Patients With Chronic Kidney Disease].

    PubMed

    Chen, Jui-O; Lin, Chiu-Chu

    2016-06-01

    The aging population and changing lifestyles have lead to the increased general risk of chronic kidney disease. Taiwan currently has the highest incidence and prevalence of end-stage renal disease (ESRD) of any country or region in the world. Hemodialysis patients must endure comorbidities and face the uncertainties of death. The best way to achieve a good death is for patients to sign advance care planning (ACP). However, the key factors contributing to low ACP signature rates have been the lack of communication skills and related training among medical staffs. This article explores the dilemma of ACP using an example of chronic kidney disease (CKD) and proposes a theory-based approach to develop a theoretical framework for an ACP simulation-situation communication training program that integrates the simulation situation model, PREPARED model, and scaffolding theory. Readers may use this framework to design ACP simulation-situation communication training programs that conform to their own conditions and then test the effectiveness and feasibility of these programs in clinical settings.

  3. [Constructing an ACP Simulation-Situation Communication Training Program for Patients With Chronic Kidney Disease].

    PubMed

    Chen, Jui-O; Lin, Chiu-Chu

    2016-06-01

    The aging population and changing lifestyles have lead to the increased general risk of chronic kidney disease. Taiwan currently has the highest incidence and prevalence of end-stage renal disease (ESRD) of any country or region in the world. Hemodialysis patients must endure comorbidities and face the uncertainties of death. The best way to achieve a good death is for patients to sign advance care planning (ACP). However, the key factors contributing to low ACP signature rates have been the lack of communication skills and related training among medical staffs. This article explores the dilemma of ACP using an example of chronic kidney disease (CKD) and proposes a theory-based approach to develop a theoretical framework for an ACP simulation-situation communication training program that integrates the simulation situation model, PREPARED model, and scaffolding theory. Readers may use this framework to design ACP simulation-situation communication training programs that conform to their own conditions and then test the effectiveness and feasibility of these programs in clinical settings. PMID:27250964

  4. The structural basis for neutrophil inactivation of C1 inhibitor.

    PubMed Central

    Pemberton, P A; Harrison, R A; Lachmann, P J; Carrell, R W

    1989-01-01

    Limited proteolysis of C1 inhibitor (C1-INH) by neutrophil elastase, Pseudomonas elastase and snake venoms resulted in initial cleavage within the molecule's N-terminus followed by further cleavage within the molecule's C-terminally placed reactive centre. N-Terminal proteolysis occurred at peptide bonds 14-15, 36-37 and 40-41. This had no effect on either the inhibitory activity or the heat-stability of C1-INH. Proteolysis within the reactive centre occurred at peptide bonds 439-440, 440-441, 441-442 and 442-443. Cleavage at any one of these sites inactivated C1-INH and conferred enhanced heat-stability upon a previously heat-labile molecule. Released neutrophil proteinases also cleaved and inactivated C1-INH, suggesting that they may physiologically regulate C1-INH during inflammatory episodes. Images Fig. 1. Fig. 3. Fig. 5. Fig. 6. PMID:2930506

  5. Constructing a resilience index for the Enhanced Critical Infrastructure Protection Program

    SciTech Connect

    Fisher, R. E.; Bassett, G. W.; Buehring, W. A.; Collins, M. J.; Dickinson, D. C.; Eaton, L. K.; Haffenden, R. A.; Hussar, N. E.; Klett, M. S.; Lawlor, M. A.; Millier, D. J.; Petit, F. D.; Peyton, S. M.; Wallace, K. E.; Whitfield, R. G.; Peerenboom, J P

    2010-10-14

    Following recommendations made in Homeland Security Presidential Directive 7, which established a national policy for the identification and increased protection of critical infrastructure and key resources (CIKR) by Federal departments and agencies, the U.S. Department of Homeland Security (DHS) in 2006 developed the Enhanced Critical Infrastructure Protection (ECIP) program. The ECIP program aimed to provide a closer partnership with state, regional, territorial, local, and tribal authorities in fulfilling the national objective to improve CIKR protection. The program was specifically designed to identify protective measures currently in place in CIKR and to inform facility owners/operators of the benefits of new protective measures. The ECIP program also sought to enhance existing relationships between DHS and owners/operators of CIKR and to build relationships where none existed (DHS 2008; DHS 2009). In 2009, DHS and its protective security advisors (PSAs) began assessing CIKR assets using the ECIP program and ultimately produced individual protective measure and vulnerability values through the protective measure and vulnerability indices (PMI/VI). The PMI/VI assess the protective measures posture of individual facilities at their 'weakest link,' allowing for a detailed analysis of the most vulnerable aspects of the facilities (Schneier 2003), while maintaining the ability to produce an overall protective measures picture. The PMI has six main components (physical security, security management, security force, information sharing, protective measures assessments, and dependencies) and focuses on actions taken by a facility to prevent or deter the occurrence of an incident (Argonne National Laboratory 2009). As CIKR continue to be assessed using the PMI/VI and owners/operators better understand how they can prevent or deter incidents, academic research, practitioner emphasis, and public policy formation have increasingly focused on resilience as a necessary

  6. In vitro interaction of C1-inhibitor with thrombin.

    PubMed

    Cugno, M; Bos, I; Lubbers, Y; Hack, C E; Agostoni, A

    2001-06-01

    Previous observations of increased generation of thrombin during acute attacks of angioedema in plasma of patients with C1-inhibitor (C1-INH) deficiency prompted us to evaluate the interaction of C1-INH with thrombin in both purified systems and human plasma. For this purpose, we used several methods: (1) sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting analysis; (2) enzyme-linked immunosorbent assays to measure complexes between C1-INH and thrombin and inactivated C1-INH; and (3) kinetic studies using a chromogenic assay. We found that the interaction of purified C1-INH with thrombin is associated with the formation of bimolecular complexes of molecular weight (Mr) 130 000 and 120 000 as well as with the appearance of a cleaved form of C1-INH of Mr 97 000. The kinetic studies of inhibition of thrombin by C1-INH showed an average second-order rate constant of 19/s per mol/l, which was significantly increased in the presence of heparin. The addition of thrombin to human plasma was not associated with detectable C1-INH-thrombin complex formation or with cleavage of C1-INH. In conclusion, our data demonstrate that C1-INH upon interaction with thrombin, in part, forms enzyme-inhibitor complexes and, in part, is cleaved. The low second-order rate constant and the lack of a significant interaction in plasma suggest that the inhibition of thrombin by C1-INH has a minor role in circulating blood; however, its role might be important at the endothelial surface, where high concentrations of glycosaminoglycans occur.

  7. 17 CFR 240.15c1-1 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 17 Commodity and Securities Exchanges 3 2010-04-01 2010-04-01 false Definitions. 240.15c1-1... Exchange Act of 1934 Rules Relating to Over-The-Counter Markets § 240.15c1-1 Definitions. As used in any rule adopted pursuant to section 15(c)(1) of the Act: (a) The term customer shall not include a...

  8. Human genes for complement components C1r and C1s in a close tail-to-tail arrangement.

    PubMed Central

    Kusumoto, H; Hirosawa, S; Salier, J P; Hagen, F S; Kurachi, K

    1988-01-01

    Complementary DNA clones for human C1s were isolated from cDNA libraries that were prepared with poly(A)+ RNAs of human liver and HepG2 cells. A clone with the largest cDNA insert of 2664 base pairs (bp) was analyzed for its complete nucleotide sequence. It contained 202 bp of a 5' untranslated region, 45 bp of coding for a signal peptide (15 amino acid residues), 2019 bp for complement component C1s zymogen (673 amino acid residues), 378 bp for a 3' untranslated region, a stop codon, and 17 bp of a poly(A) tail. The amino acid sequence of C1s was 40.5% identical to that of C1r, with excellent matches of tentative disulfide bond locations conserving the overall domain structure of C1r. DNA blotting and sequencing analyses of genomic DNA and of an isolated genomic DNA clone clearly showed that the human genes for C1r and C1s are closely located in a "tail-to-tail" arrangement at a distance of about 9.5 kilobases. Furthermore, RNA blot analyses showed that both C1r and C1s genes are primarily expressed in liver, whereas most other tissues expressed both C1r and C1s genes at much lower levels (less than 10% of that in liver). Multiple molecular sizes of specific mRNAs were observed in the RNA blot analyses for both C1r and C1s, indicating that alternative RNA processing(s), likely an alternative polyadenylylation, might take place for both genes. Images PMID:2459702

  9. C1q rs292001 polymorphism and C1q antibodies in juvenile lupus and their relation to lupus nephritis

    PubMed Central

    Mosaad, Y M; Hammad, A; Fawzy, Z; El-Refaaey, A; Tawhid, Z; Hammad, E M; Youssef, L F; ElAttar, E A A; Radwan, D F; Fawzy, I M

    2015-01-01

    C1q deficiency is related strongly to systemic lupus erythematosus (SLE), but very few and inconsistent studies explored the single nucleotide polymorphisms of the C1q gene in relation to juvenile SLE (jSLE) and lupus nephritis (LN). The objective of this study was to analyse whether C1q rs 292001 polymorphism is associated with SLE and disease phenotype, especially nephritis, and to investigate the relation between this polymorphism and clinical data, treatment outcome, serum level of C1q protein and antibodies. Typing of C1q rs292001 polymorphism using restriction fragment length polymorphism and measuring serum levels of C1q protein and antibodies by enzyme-linked immunosorbent assay (ELISA) were performed for 130 children with SLE and 208 healthy controls. The A allele of C1q rs292001 was associated with jSLE and LN (P = 0·005 and 0·013, respectively) and the AA genotype was associated with jSLE (P = 0·036). Low serum levels of C1q protein were found in jSLE and LN (P < 0·001 and 0·009, respectively), and these levels were increased after treatment in patients with LN (P = 0·009) and active renal disease (P = 0·027). Higher titres of C1q antibodies were found in patients with LN (P = 0·015) and correlated negatively with C1q protein level (P < 0·001) and patient age (P = 0·04). The A allele and AA genotype of C1q rs292001 can be considered a susceptibility risk factor and the GG genotype could be considered protective for jSLE and LN in the studied cohort of Egyptian children. Decreased serum levels of C1q protein and increased titres of C1q antibodies may be involved in the pathogenesis of jSLE, especially LN. PMID:26095468

  10. Nash equilibrium in differential games and the construction of the programmed iteration method

    SciTech Connect

    Averboukh, Yurii V

    2011-05-31

    This work is devoted to the study of nonzero-sum differential games. The set of payoffs in a situation of Nash equilibrium is examined. It is shown that the set of payoffs in a situation of Nash equilibrium coincides with the set of values of consistent functions which are fixed points of the program absorption operator. A condition for functions to be consistent is given in terms of the weak invariance of the graph of the functions under a certain differential inclusion. Bibliography: 18 titles.

  11. Nash equilibrium in differential games and the construction of the programmed iteration method

    NASA Astrophysics Data System (ADS)

    Averboukh, Yurii V.

    2011-05-01

    This work is devoted to the study of nonzero-sum differential games. The set of payoffs in a situation of Nash equilibrium is examined. It is shown that the set of payoffs in a situation of Nash equilibrium coincides with the set of values of consistent functions which are fixed points of the program absorption operator. A condition for functions to be consistent is given in terms of the weak invariance of the graph of the functions under a certain differential inclusion. Bibliography: 18 titles.

  12. New residential construction compliance: Evaluation of the Washington State Energy Code program

    SciTech Connect

    Warwick, W.M.; Lee, A.D.; Sandahl, L.J.; Durfee, D.L.; Richman, E.E.

    1993-07-01

    This report describes the Pacific Northwest Laboratory`s (PNL`s) evaluation of the Washington State Energy Code Program (WSECP). In 1990, the Washington State Legislature passed a residential energy efficiency code to be effective July 1, 1992. Bonneville supported passage and implementation of the code to ensure that new residences in the State of Washington were as energy efficient as economically feasible. The Washington State Energy Office (WSEO) is conducting the WSECP for Bonneville to support code implementation. This support takes several forms, including providing training to code enforcement officials, technical support both in the field and through telephone ``hot lines,`` and computerized tools to review house plans for code compliance. WSEO began implementing the WSECP in 1992, prior to the effective date of the new code. This first phase of the WSECP was the subject of an earlier process evaluation conducted by PNL. From that evaluation PNL found that most new homes being built immediately after the code went into effect were ``grand-fathered`` under the old code. The training program for the new code was in place and sessions were being attended by the jurisdictions but it was too early to determine if the training was effective in improving code compliance and easing the transition to the new energy code. That is the subject of this evaluation.

  13. Shuttle Rocket Motor Program: NASA should delay awarding some construction contracts. Report to the Chair, Subcommittee on Government Activities and Transportation, Committee on Government Operations, House of Representatives

    NASA Technical Reports Server (NTRS)

    1992-01-01

    Even though the executive branch has proposed terminating the Advanced Solid Rocket Motor (ASRM) program, NASA is proceeding with all construction activity planned for FY 1992 to avoid schedule slippage if the program is reinstated by Congress. However, NASA could delay some construction activities for at least a few months without affecting the current launch data schedule. For example, NASA could delay Yellow Creek's motor storage and dock projects, Stennis' dock project, and Kennedy's rotation processing and surge facility and dock projects. Starting all construction activities as originally planned could result in unnecessarily incurring additional costs and termination liability if the funding for FY 1993 is not provided. If Congress decides to continue the program, construction could still be completed in time to avoid schedule slippage.

  14. A linear semi-infinite programming strategy for constructing optimal wavelet transforms in multivariate calibration problems.

    PubMed

    Coelho, Clarimar José; Galvão, Roberto K H; de Araújo, Mário César U; Pimentel, Maria Fernanda; da Silva, Edvan Cirino

    2003-01-01

    A novel strategy for the optimization of wavelet transforms with respect to the statistics of the data set in multivariate calibration problems is proposed. The optimization follows a linear semi-infinite programming formulation, which does not display local maxima problems and can be reproducibly solved with modest computational effort. After the optimization, a variable selection algorithm is employed to choose a subset of wavelet coefficients with minimal collinearity. The selection allows the building of a calibration model by direct multiple linear regression on the wavelet coefficients. In an illustrative application involving the simultaneous determination of Mn, Mo, Cr, Ni, and Fe in steel samples by ICP-AES, the proposed strategy yielded more accurate predictions than PCR, PLS, and nonoptimized wavelet regression. PMID:12767151

  15. 26 CFR 1.662(c)-1 - Different taxable years.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 26 Internal Revenue 8 2012-04-01 2012-04-01 false Different taxable years. 1.662(c)-1 Section 1... Corpus § 1.662(c)-1 Different taxable years. If a beneficiary has a different taxable year (as defined in section 441 or 442) from the taxable year of an estate or trust, the amount he is required to include...

  16. 26 CFR 1.662(c)-1 - Different taxable years.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 26 Internal Revenue 8 2014-04-01 2014-04-01 false Different taxable years. 1.662(c)-1 Section 1... Corpus § 1.662(c)-1 Different taxable years. If a beneficiary has a different taxable year (as defined in section 441 or 442) from the taxable year of an estate or trust, the amount he is required to include...

  17. 26 CFR 1.662(c)-1 - Different taxable years.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 26 Internal Revenue 8 2011-04-01 2011-04-01 false Different taxable years. 1.662(c)-1 Section 1... Corpus § 1.662(c)-1 Different taxable years. If a beneficiary has a different taxable year (as defined in section 441 or 442) from the taxable year of an estate or trust, the amount he is required to include...

  18. 26 CFR 1.652(c)-1 - Different taxable years.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 8 2010-04-01 2010-04-01 false Different taxable years. 1.652(c)-1 Section 1... (CONTINUED) INCOME TAXES Trusts Which Distribute Current Income Only § 1.652(c)-1 Different taxable years. If a beneficiary has a different taxable year (as defined in section 441 or 442) from the taxable...

  19. Expression of functional human C1 inhibitor in COS cells.

    PubMed

    Eldering, E; Nuijens, J H; Hack, C E

    1988-08-25

    Full length human C1 inhibitor cDNA was cloned into a vector suitable for transient expression in COS-1 cells. Transfected COS cells secreted an immunoreactive protein of Mr approximately 110,000 that appeared to be functionally equivalent to the plasma-derived protein as established by the following criteria: 1) ability to form sodium dodecyl sulfate-stable complexes with C1s, factor XIIa, and kallikrein; 2) inhibition of C1s-mediated C4 consumption; and 3) susceptibility to inactivation by the nontarget proteinase elastase. Quantitation of secreted recombinant C1 inhibitor by radioimmunoassay indicated that 72 h after transfection the level was approximately 2.2 micrograms/ml. Treatment of transfected cells with tunicamycin resulted in secretion of a protein of Mr approximately 90,000 that was also capable of complex formation with C1s.

  20. Are C1 chondrites chemically fractionated - A trace element study

    NASA Technical Reports Server (NTRS)

    Ebihara, M.; Wolf, R.; Anders, E.

    1982-01-01

    Six C1 chondrite samples and a C2 xenolith from the Plainview H5 chondrite were analyzed by radiochemical neutron activation for a large variety of elements, including rare earths. The sample processing is described, including the irradiation, chemical procedure, rare earths separation, counting techniques, radiochemical purity check, and chemical yields. The results of consistency checks on a number of elements are discussed. Abundances for siderophiles, volatiles, and rare earths are presented and discussed. Tests are presented for fractionation of rare earths and other refractories, compositional uniformity of C1's, and interelement correlations. There is no conclusive evidence for nebular fractionation affecting C1's. Three fractionation-prone rare earths have essentially the same relative abundances in C1's and all other chondrite classes, and hence are apparently not fractionated in C1's.

  1. Construction and validation of two parent-report scales for the evaluation of early intervention programs.

    PubMed

    Fisher, William P; Elbaum, Batya; Coulter, W Alan

    2012-01-01

    The State Performance Plan (SPP) developed under the 2004 reauthorization of the Individuals with Disabilities Education Act (IDEA 2004, Public Law 108-446) requires states to collect data and report on the impact of early intervention services on three key outcomes for participating families. The NCSEAM Impact on Family Scale (NIFS) and the NCSEAM Family Centered Services Scale (NFCSS) were developed to provide states with a means to address this new reporting requirement and to collect additional data that would inform program improvement efforts. Items suggested by stakeholder groups were piloted with a nationally representative sample of parents of children with developmental delays or disabilities ages birth to three participating in early intervention services in eight states. The 28-item NIFS had measurement reliabilities ranging from .93-.96 in a sample of 1,750; measurement reliabilities for the 135-item NFCSS ranged from .94 to .97 in a sample of 1,755 respondents. A 29-item version of the NFCSS had measurement reliabilities ranging from .87 to .92. Using data from the pilot study, stakeholders established a recommended performance standard, set at a meaningful point in the NIFS item hierarchy, for each of the three established outcome areas. PMID:22677497

  2. [Partnership between Psychosocial Care Center and Family Health Program: the challenge of a new knowledge construction].

    PubMed

    Delfini, Patrícia Santos de Souza; Sato, Miki Takao; Antoneli, Patrícia de Paulo; Guimarães, Paulo Octávio da Silva

    2009-10-01

    The objective of the article is to report an experience of partnership between a Psychosocial Care Center and three teams of the Family Health Program in the central region of São Paulo city. theoretical concepts like territory, subject, subjectivity/collective, receptiveness, bond, co-responsibility as well as the psychiatric and sanitary reform principles are the base and guide of this work. The partnership aims the promotion of mental health care based on articulated actions from different services. This way, the PSF's and mental health's workers are responsible for the demands of a territory. The strategies used in this partnership were meetings with both teams with training, case discussion about the families assisted, support to workers' difficulties and elaboration of therapeutical projects, and joint domiciliary visits. Some difficulties had been found during the research: great demand for health services and lack of institutional guidelines to guarantee the effectiveness of the partnership. The look directed to the family and the social context presents positive results compared to the look directed only to the illness. The partnership enriches the practice and a larger network of care in the territory becomes possible. It is necessary to bring up new proposals and innovative enterprises.

  3. Nanofiltered C1 esterase inhibitor (human) for hereditary angioedema attacks in pregnant women.

    PubMed

    Baker, James W; Craig, Timothy J; Riedl, Marc A; Banerji, Aleena; Fitts, David; Kalfus, Ira N; Uknis, Marc E

    2013-01-01

    Data are limited on hereditary angioedema (HAE) in pregnant women and the safety and efficacy of therapies for treatment and prevention of HAE attacks during pregnancy. Prospective studies are unlikely given the rarity of HAE and ethical considerations regarding enrollment of pregnant female subjects in clinical trials. A retrospective analysis of clinical trial and compassionate-use data was conducted to identify subjects who received nanofiltered C1 esterase inhibitor (C1 INH-nf; human) during pregnancy. This study evaluates the efficacy and safety of human C1 INH-nf for treatment and prevention of HAE attacks in pregnant women. Data from pregnant subjects enrolled in either open-label extensions of two randomized, double-blind, placebo-controlled trials of C1 INH-nf or in a compassionate-use program were retrospectively analyzed for efficacy (e.g., total attacks, attack frequency during prophylaxis, and monthly attack rates) and safety (e.g., pregnancy outcomes and adverse events). C1 INH-nf was administered as acute treatment, preprocedural prophylaxis, or routine prophylaxis. C1 INH-nf prophylaxis substantially reduced monthly attack rates. Of 16 subjects, 13 delivered 14 healthy neonates (1 set of twins). Two adverse fetal outcomes were reported; neither was considered by the principal investigator to be related to C1 INH-nf. One subject's pregnancy outcome was unknown. This analysis shows a favorable risk-benefit profile for C1 INH-nf for managing HAE during pregnancy. NCT Identifier: NCT00438815; NCT00462709. PMID:23484892

  4. Constructing vulnerabilty and protective measures indices for the enhanced critical infrastructure protection program.

    SciTech Connect

    Fisher, R. E.; Buehring, W. A.; Whitfield, R. G.; Bassett, G. W.; Dickinson, D. C.; Haffenden, R. A.; Klett, M. S.; Lawlor, M. A.; Decision and Information Sciences; LANL

    2009-10-14

    The US Department of Homeland Security (DHS) has directed its Protective Security Advisors (PSAs) to form partnerships with the owners and operators of assets most essential to the Nation's well being - a subclass of critical infrastructure and key resources (CIKR) - and to conduct site visits for these and other high-risk assets as part of the Enhanced Critical Infrastructure Protection (ECIP) Program. During each such visit, the PSA documents information about the facility's current CIKR protection posture and overall security awareness. The primary goals for ECIP site visits (DHS 2009) are to: (1) inform facility owners and operators of the importance of their facilities as an identified high-priority CIKR and the need to be vigilant in light of the ever-present threat of terrorism; (2) identify protective measures currently in place at these facilities, provide comparisons of CIKR protection postures across like assets, and track the implementation of new protective measures; and (3) enhance existing relationships among facility owners and operators; DHS; and various Federal, State, local tribal, and territorial partners. PSAs conduct ECIP visits to assess overall site security; educate facility owners and operators about security; help owners and operators identify gaps and potential improvements; and promote communication and information sharing among facility owners and operators, DHS, State governments, and other security partners. Information collected during ECIP visits is used to develop metrics; conduct sector-by-sector and cross-sector vulnerability comparisons; identify security gaps and trends across CIKR sectors and subsectors; establish sector baseline security survey results; and track progress toward improving CIKR security through activities, programs, outreach, and training (Snyder 2009). The data being collected are used in a framework consistent with the National Infrastructure Protection Plan (NIPP) risk criteria (DHS 2009). The NIPP

  5. Programming Constructs for Exascale Computing in Support of Space Situational Awareness

    NASA Astrophysics Data System (ADS)

    Schmalz, M.; Ranka, S.; Hayden, E.; Chapman, W.; Sahni, S.; Ritter, G.

    2013-09-01

    Increasing image and signal data burden associated with astronomical image processing in support of space situational awareness implies much-needed growth of computational throughput beyond petascale (1015 FLOP/s) to exascale regimes (1018 FLOP/s, 1018 bytes of memory, 1018 disks and Input/Output (I/O) channels, etc.) In addition to growth in applications data burden and diversity, the breadth and diversity of high performance computing architectures and their various organizations have confounded the development of a single, unifying, practicable model of parallel computation. Therefore, models for parallel Exa Scale processing have leveraged architectural and structural idiosyncrasies, yielding potential misapplications. In response to this challenge, we have developed a concise, efficient computational paradigm and software called Parallel Computing with Exascale Mapping (PCEM) to facilitate efficient mapping of annotated application codes to parallel exascale processors. Our theory, algorithms, software, and experimental results support annotation-based parallelization of application codes for envisioned exascale architectures, based on Image Algebra (IA) [Rit01]. Because of the rigor, completeness, conciseness, and layered design of image algebra notation, application-to-architecture mapping is feasible and scalable for exascale architectures. In particular, parallel operations and programs can be categorized in terms of six types of parallel operations - each type is mapped to heterogeneous exascale processors via simple rules in the PCEM annotation language. In this paper, we overview the opportunities and challenges of exascale computing for image and signal computing in support of astronomical image processing in space situational awareness applications. We discuss software interfaces and several demonstration applications, with performance analysis and results in terms of execution time as well as storage, power, and energy consumption for bus

  6. A neoepitope-based enzyme immunoassay for quantification of C1-inhibitor in complex with C1r and C1s.

    PubMed

    Fure, H; Nielsen, E W; Hack, C E; Mollnes, T E

    1997-12-01

    Monoclonal antibodies (MoAb) recognizing neoepitopes exposed on activation products of complement proteins but hidden in the native components have been used for quantification of activated complement. A previously produced and characterized mouse MoAb, recognizing a neoepitope on the human plasma protein C1-inhibitor complexed with its substrates, was used to design an enzyme immunoassay for detection of C1-inhibitor complexed with C1r and C1s. These complexes are indicators of early classical complement pathway activation. The standard was serum activated with heat aggregated IgG defined to contain 1000 arbitrary units (AU)/ml. The lower detection limit was approximately 0.05 AU/ml corresponding to 0.005% of fully activated serum. The reliability of the assay, including day-to-day variation, was tested. Intra-assay variation coefficients were 12% for low plasma control and 13% for high plasma control (n = 12 for both). Inter-assay variation coefficients were 12% for low control (n = 6), 19% for high control (n = 6) and 15% for the normal plasma control (n = 9). A 2.5-97.5 percentile reference range (normal blood donors) was 16-33 AU/ml. Two patients with systemic lupus erythematosus had considerably elevated plasma levels of the activation product (56 and 62 AU/ml), and six patients with hereditary angioedema had normal plasma levels despite considerably reduced C1-inhibitor concentration. We conclude that the present method is sensitive and reliable for detection of early classical pathway activation and superior to previously published methods by utilizing neoepitope specificity and non-radiolabelled reagents.

  7. [Interaction of Arenicin-1 with C1q Protein].

    PubMed

    Berlov, M N; Umnyakova, E S; Leonova, T S; Milman, B L; Krasnodembskaya, A D; Ovchinnikova, T V; Kokryakov, V N

    2015-01-01

    The interaction between arenicin-1, that is an antimicrobial peptide from polychaeta Arenicola marina, and human complement system protein C1q was studied using enzyme-linked receptor sorbent assay and ELISA. We revealed that arenicin-1 and C1q form complex that is stable in high ionic strength condition 0.5 M NaCl. The ability of C1q to interact with arenicin-1 is comparable with the binding activity of C1q towards another antimicrobial peptide, porcine cathelicidin protegrin-1, which has a similar spatial arrangement with arenicin-1. Namely, both arenicin-1 and protegrin-1 form cystine-stabilized antiparallel β-hairpin structure. PMID:27125019

  8. Treatment of type I and II hereditary angioedema with Rhucin, a recombinant human C1 inhibitor.

    PubMed

    Varga, Lilian; Farkas, Henriette

    2008-11-01

    Hereditary and acquired angioedema are of outstanding clinical importance, as edematous attacks associated with these conditions can thrust afflicted patients into mortal danger. Currently, C1 inhibitor concentrate - a human blood product - is available as a replacement therapy. In view of the limited number of donors, as well as the risk of transmission of blood-borne infections, it is a reasonable expectation to develop a therapeutic alternative based on recombinant technology, which would eliminate all these shortcomings. Pharming (Leiden, The Netherlands) has developed Rhucin, a recombinant human C1 inhibitor, as a proprietary product, which is currently being evaluated in Phase III clinical trials. Ongoing studies conducted within the framework of the development program are almost complete and their interim findings are reassuring. This should facilitate successful regulatory approval in the near future, which is indispensable in order to make Rhucin available for patients with hereditary angioedema or other disorders amenable to C1 inhibitor replacement.

  9. Treatment of type I and II hereditary angioedema with Rhucin, a recombinant human C1 inhibitor.

    PubMed

    Varga, Lilian; Farkas, Henriette

    2008-11-01

    Hereditary and acquired angioedema are of outstanding clinical importance, as edematous attacks associated with these conditions can thrust afflicted patients into mortal danger. Currently, C1 inhibitor concentrate - a human blood product - is available as a replacement therapy. In view of the limited number of donors, as well as the risk of transmission of blood-borne infections, it is a reasonable expectation to develop a therapeutic alternative based on recombinant technology, which would eliminate all these shortcomings. Pharming (Leiden, The Netherlands) has developed Rhucin, a recombinant human C1 inhibitor, as a proprietary product, which is currently being evaluated in Phase III clinical trials. Ongoing studies conducted within the framework of the development program are almost complete and their interim findings are reassuring. This should facilitate successful regulatory approval in the near future, which is indispensable in order to make Rhucin available for patients with hereditary angioedema or other disorders amenable to C1 inhibitor replacement. PMID:20477114

  10. 26 CFR 1.1402(c)-1 - Trade or business.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 12 2010-04-01 2010-04-01 false Trade or business. 1.1402(c)-1 Section 1.1402(c... (CONTINUED) INCOME TAXES Tax on Self-Employment Income § 1.1402(c)-1 Trade or business. In order for an individual to have net earnings from self-employment, he must carry on a trade or business, either as...

  11. Potential Roles for C1 Inhibitor in Transplantation.

    PubMed

    Berger, Mel; Baldwin, William M; Jordan, Stanley C

    2016-07-01

    Complement is a major contributor to inflammation and graft injury. This system is especially important in ischemia-reperfusion injury/delayed graft function as well as in acute and chronic antibody-mediated rejection (AMR). The latter is increasingly recognized as a major cause of late graft loss, for which we have few effective therapies. C1 inhibitor (C1-INH) regulates several pathways which contribute to both acute and chronic graft injuries. However, C1-INH spares the alternative pathway and the membrane attack complex (C5-9) so innate antibacterial defenses remain intact. Plasma-derived C1-INH has been used to treat hereditary angioedema for more than 30 years with excellent safety. Studies with C1-INH in transplant recipients are limited, but have not revealed any unique toxicity or serious adverse events attributed to the protein. Extensive data from animal and ex vivo models suggest that C1-INH ameliorates ischemia-reperfusion injury. Initial clinical studies suggest this effect may allow transplantation of donor organs which are now discarded because the risk of primary graft dysfunction is considered too great. Although the incidence of severe early AMR is declining, accumulating evidence strongly suggests that complement is an important mediator of chronic AMR, a major cause of late graft loss. Thus, C1-INH may also be helpful in preserving function of established grafts. Early clinical studies in transplantation suggest significant beneficial effects of C1-INH with minimal toxicity. Recent results encourage continued investigation of this already-available therapeutic agent.

  12. 26 CFR 1.1402(c)-1 - Trade or business.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 26 Internal Revenue 12 2013-04-01 2013-04-01 false Trade or business. 1.1402(c)-1 Section 1.1402(c... (CONTINUED) INCOME TAXES (CONTINUED) Tax on Self-Employment Income § 1.1402(c)-1 Trade or business. In order for an individual to have net earnings from self-employment, he must carry on a trade or...

  13. 26 CFR 1.1402(c)-1 - Trade or business.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 26 Internal Revenue 12 2012-04-01 2012-04-01 false Trade or business. 1.1402(c)-1 Section 1.1402(c... (CONTINUED) INCOME TAXES (CONTINUED) Tax on Self-Employment Income § 1.1402(c)-1 Trade or business. In order for an individual to have net earnings from self-employment, he must carry on a trade or...

  14. Uncoupling complement C1s activation from C1q binding in apoptotic cell phagocytosis and immunosuppressive capacity.

    PubMed

    Colonna, Lucrezia; Parry, Graham C; Panicker, Sandip; Elkon, Keith B

    2016-02-01

    Complement activation contributes to inflammation in many diseases, yet it also supports physiologic apoptotic cells (AC) clearance and its downstream immunosuppressive effects. The roles of individual complement components in AC phagocytosis have been difficult to dissect with artificially depleted sera. Using human in vitro systems and the novel antibody complement C1s inhibitor TNT003, we uncoupled the role of the enzymatic activation of the classical pathway from the opsonizing role of C1q in mediating a) the phagocytosis of early and late AC, and b) the immunosuppressive capacity of early AC. We found that C1s inhibition had a small impact on the physiologic clearance of early AC, leaving their immunosuppressive properties entirely unaffected, while mainly inhibiting the phagocytosis of late apoptotic/secondary necrotic cells. Our data suggest that C1s inhibition may represent a valuable therapeutic strategy to control classical pathway activation without causing significant AC accumulation in diseases without defects in AC phagocytosis.

  15. C1-Pathways in Methyloversatilis universalis FAM5: Genome Wide Gene Expression and Mutagenesis Studies

    PubMed Central

    Good, Nathan M.; Lamb, Andrew; Beck, David A. C.; Martinez-Gomez, N. Cecilia; Kalyuzhnaya, Marina G.

    2015-01-01

    Methyloversatilis universalis FAM5 utilizes single carbon compounds such as methanol or methylamine as a sole source of carbon and energy. Expression profiling reveals distinct sets of genes altered during growth on methylamine vs methanol. As expected, all genes for the N-methylglutamate pathway were induced during growth on methylamine. Among other functions responding to the aminated source of C1-carbon, are a heme-containing amine dehydrogenase (Qhp), a distant homologue of formaldehyde activating enzyme (Fae3), molybdenum-containing formate dehydrogenase, ferredoxin reductase, a set of homologues to urea/ammonium transporters and amino-acid permeases. Mutants lacking one of the functional subunits of the amine dehydrogenase (ΔqhpA) or Δfae3 showed no growth defect on C1-compounds. M. universalis FAM5 strains with a lesion in the H4-folate pathway were not able to use any C1-compound, methanol or methylamine. Genes essential for C1-assimilation (the serine cycle and glyoxylate shunt) and H4MTP-pathway for formaldehyde oxidation showed similar levels of expression on both C1-carbon sources. M. universalis FAM5 possesses three homologs of the formaldehyde activating enzyme, a key enzyme of the H4MTP-pathway. Strains lacking the canonical Fae (fae1) lost the ability to grow on both C1-compounds. However, upon incubation on methylamine the fae1-mutant produced revertants (Δfae1R), which regained the ability to grow on methylamine. Double and triple mutants (Δfae1RΔfae3, or Δfae1RΔfae2 or Δfae1RΔfae2Δfae3) constructed in the revertant strain background showed growth similar to the Δfae1R phenotype. The metabolic pathways for utilization of methanol and methylamine in Methyloversatilis universalis FAM5 are reconstructed based on these gene expression and phenotypic data.

  16. C1-Pathways in Methyloversatilis universalis FAM5: Genome Wide Gene Expression and Mutagenesis Studies

    PubMed Central

    Good, Nathan M.; Lamb, Andrew; Beck, David A. C.; Martinez-Gomez, N. Cecilia; Kalyuzhnaya, Marina G.

    2015-01-01

    Methyloversatilis universalis FAM5 utilizes single carbon compounds such as methanol or methylamine as a sole source of carbon and energy. Expression profiling reveals distinct sets of genes altered during growth on methylamine vs methanol. As expected, all genes for the N-methylglutamate pathway were induced during growth on methylamine. Among other functions responding to the aminated source of C1-carbon, are a heme-containing amine dehydrogenase (Qhp), a distant homologue of formaldehyde activating enzyme (Fae3), molybdenum-containing formate dehydrogenase, ferredoxin reductase, a set of homologues to urea/ammonium transporters and amino-acid permeases. Mutants lacking one of the functional subunits of the amine dehydrogenase (ΔqhpA) or Δfae3 showed no growth defect on C1-compounds. M. universalis FAM5 strains with a lesion in the H4-folate pathway were not able to use any C1-compound, methanol or methylamine. Genes essential for C1-assimilation (the serine cycle and glyoxylate shunt) and H4MTP-pathway for formaldehyde oxidation showed similar levels of expression on both C1-carbon sources. M. universalis FAM5 possesses three homologs of the formaldehyde activating enzyme, a key enzyme of the H4MTP-pathway. Strains lacking the canonical Fae (fae1) lost the ability to grow on both C1-compounds. However, upon incubation on methylamine the fae1-mutant produced revertants (Δfae1R), which regained the ability to grow on methylamine. Double and triple mutants (Δfae1RΔfae3, or Δfae1RΔfae2 or Δfae1RΔfae2Δfae3) constructed in the revertant strain background showed growth similar to the Δfae1R phenotype. The metabolic pathways for utilization of methanol and methylamine in Methyloversatilis universalis FAM5 are reconstructed based on these gene expression and phenotypic data. PMID:27682085

  17. Combination of skull traction with posterior C1-2 fusion for old C1-2 dislocations.

    PubMed

    Li, Xue Feng; Yang, Hui Lin; Jiang, Wei Min; Tang, Tian Si; Gong, Xiao Hong; Yuan, Jing; Zhang, Ji Gang; Wang, Gen Lin

    2011-06-01

    Between January 2003 and December 2009, 23 patients who had suffered old C1-2 dislocations, were surgically treated in our orthopedics department. Fifteen patients underwent direct posterior C1-2 fusion following pre-operative reduction by skull traction. In eight patients, reduction was achieved only by skull traction under general anesthesia, facilitated by manual hyperextension of the cervical spine and maintained by simultaneous posterior C1-2 fusion. Intra-operative traction was monitored using C-arm fluoroscopy and cortical somatosensory-evoked potentials. Posterior C1-2 fixation was achieved in nine patients using C1-2 laminar hooks and in 14 patients using C1 laminar hooks with C2 pedicle screws. During the follow-up of 5 to 72 months (mean: 42.8 months), solid bony fusion was accomplished in all patients. Using Di Lorenzo's grades and Japanese Orthopedics Association scores, there was significant improvement (p<0.05). The cervical medullary angle exhibited a significant improvement of 31.7°, from 121.6° to 153.3° (p<0.05). There were no complications, including dural tears, spinal cord damage, vertebral artery damage, or breakage or loosening of implants.

  18. Emerging and Novel Functions of Complement Protein C1q

    PubMed Central

    Kouser, Lubna; Madhukaran, Shanmuga Priyaa; Shastri, Abhishek; Saraon, Anuvinder; Ferluga, Janez; Al-Mozaini, Maha; Kishore, Uday

    2015-01-01

    Complement protein C1q, the recognition molecule of the classical pathway, performs a diverse range of complement and non-complement functions. It can bind various ligands derived from self, non-self, and altered self and modulate the functions of immune and non-immune cells including dendritic cells and microglia. C1q involvement in the clearance of apoptotic cells and subsequent B cell tolerance is more established now. Recent evidence appears to suggest that C1q plays an important role in pregnancy where its deficiency and dysregulation can have adverse effects, leading to preeclampsia, missed abortion, miscarriage or spontaneous loss, and various infections. C1q is also produced locally in the central nervous system, and has a protective role against pathogens and possible inflammatory functions while interacting with aggregated proteins leading to neurodegenerative diseases. C1q role in synaptic pruning, and thus CNS development, its anti-cancer effects as an immune surveillance molecule, and possibly in aging are currently areas of extensive research. PMID:26175731

  19. Self-Help Construction of 1-Story Buildings. Appropriate Technologies for Development. Program & Training Journal Manual Series, Number 6.

    ERIC Educational Resources Information Center

    Gallant, Peter

    This manual is designed to assist Peace Corps field workers who have little or no construction experience in helping communities or families to construct one-story buildings. Discussed in a chapter on basic planning and design are site and position; size, shape, and floor plan; and construction materials. The next chapter is a detailed examination…

  20. Engagement in a Community of Learners as a Mediating Agent toward the Construction of Technological Pedagogical Content Knowledge (TPACK) in an Online Master's Program

    ERIC Educational Resources Information Center

    Gillow-Wiles, Henry

    2012-01-01

    This study investigates how teachers develop and extend their understanding and knowledge of teaching and learning with digital technologies in a primarily online Master of Science program. The investigation focuses on exploring the relationship between developing an online community of learners and the construction of technological pedagogical…

  1. Inactivation of C-1 inhibitor by proteases: demonstration by a monoclonal antibody of a neodeterminant on inactivated, non-complexed C-1 inhibitor.

    PubMed

    Nuijens, J H; Huijbregts, C C; van Mierlo, G M; Hack, C E

    1987-07-01

    Monoclonal antibodies were raised against kallikrein-C-1 inhibitor and factor XIIa-C-1 inhibitor complexes. One of the monoclonal antibodies (KII) appeared to react predominantly with C-1 inhibitor complexes in an ELISA. However, the apparent binding of KII to C-1 inhibitor complexes was probably due to the presence of proteolytically inactivated C-1 inhibitor in the complex mixture used for the coating:KII did not bind either kallikrein-C-1 inhibitor or factor XIIa-C-1 inhibitor complexes generated in plasma by dextran sulphate. SDS-PAGE analysis of C-1 inhibitor incubated with proteases revealed that KII-reactive C-1 inhibitor has a lower molecular weight than native C-1 inhibitor. We propose that the determinant that reacts with KII is exposed after cleavage of C-1 inhibitor in its reactive site. The monoclonal antibody KII will enable us to study the inactivation of C-1 inhibitor in human inflammatory disease.

  2. 26 CFR 1.267(c)-1 - Constructive ownership of stock.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... indirectly by or for a corporation, partnership, estate, or trust shall be considered as actual ownership of... descendants includes children and grandchildren. (b) Examples. The application of section 267(c) may be... X, and A's partner, AP, owned 60 percent in value of the outstanding stock of X. The...

  3. 26 CFR 1.267(c)-1 - Constructive ownership of stock.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... indirectly by or for a corporation, partnership, estate, or trust shall be considered as actual ownership of... descendants includes children and grandchildren. (b) Examples. The application of section 267(c) may be... X, and A's partner, AP, owned 60 percent in value of the outstanding stock of X. The...

  4. Project W-320, 241-C-106 sluicing: Construction specification W-320-C1

    SciTech Connect

    Bailey, J.W.

    1998-07-20

    Project W-320, Waste Retrieval Sluicing System (WRSS), specification is for procurement, fabrication and installation of equipment at the C Tank Farm, including Operator Station and some equipment just outside the C Tank Farm fence, necessary to support the sluicing operation. Work consists of furnishing labor, equipment, and materials to provide the means to procure materials and equipment, fabricate items, excavate and place concrete, and install equipment, piping, wiring, and structures in accordance with the Contract Documents. Major work elements include: Excavation for process and fire protection piping, electrical conduit trenches, and foundations for small structures; Placement of concrete cover blocks, foundations, and equipment pads; Procurement and installation of double walled piping, electrical conduit, fire and raw water piping, chilled water piping, and electrical cable; Procurement and installation of above-ground ventilation system piping between the (HVAC) Process building and Tank C-106; Core drill existing concrete; Furnish and installation of electrical distribution equipment; Installation of the concrete foundation, and assembly installation of the two Seismic Shutdown Systems with Environmental Enclosures; Fabrication and installation of in-pit pipe jumpers, including related valves, instruments and wiring; and Installation of a vertical submersible pump, horizontal booster pump, and winch assembly into tank access riser pits.

  5. Report to the Board of Regents State University System of Florida. Review of Programs: Architecture, Architectural Technology, Landscape Architecture, Interior Design, Construction and Construction Technology, Building Construction, Urban and Regional Planning.

    ERIC Educational Resources Information Center

    McMinn, William G.

    An evaluation and report was done on the status of programs in architecture and related fields in the Florida State University System as a follow-up to a 1983 evaluation. The evaluation involved self-studies prepared by each program and a series of site visits to each of seven campuses and two centers with programs under review. These institutions…

  6. 26 CFR 1.652(c)-1 - Different taxable years.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 26 Internal Revenue 8 2011-04-01 2011-04-01 false Different taxable years. 1.652(c)-1 Section 1... taxable years. If a beneficiary has a different taxable year (as defined in section 441 or 442) from the taxable year of the trust, the amount he is required to include in gross income in accordance with...

  7. 26 CFR 1.652(c)-1 - Different taxable years.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 26 Internal Revenue 8 2012-04-01 2012-04-01 false Different taxable years. 1.652(c)-1 Section 1... taxable years. If a beneficiary has a different taxable year (as defined in section 441 or 442) from the taxable year of the trust, the amount he is required to include in gross income in accordance with...

  8. 26 CFR 1.662(c)-1 - Different taxable years.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 8 2010-04-01 2010-04-01 false Different taxable years. 1.662(c)-1 Section 1...)-1 Different taxable years. If a beneficiary has a different taxable year (as defined in section 441 or 442) from the taxable year of an estate or trust, the amount he is required to include in...

  9. 26 CFR 1.652(c)-1 - Different taxable years.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 26 Internal Revenue 8 2014-04-01 2014-04-01 false Different taxable years. 1.652(c)-1 Section 1... taxable years. If a beneficiary has a different taxable year (as defined in section 441 or 442) from the taxable year of the trust, the amount he is required to include in gross income in accordance with...

  10. 26 CFR 1.652(c)-1 - Different taxable years.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 26 Internal Revenue 8 2013-04-01 2013-04-01 false Different taxable years. 1.652(c)-1 Section 1... taxable years. If a beneficiary has a different taxable year (as defined in section 441 or 442) from the taxable year of the trust, the amount he is required to include in gross income in accordance with...

  11. 26 CFR 1.678(c)-1 - Trusts for support.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... (CONTINUED) INCOME TAXES Grantors and Others Treated As Substantial Owners § 1.678(c)-1 Trusts for support... cotrustee, to apply the income of the trust to the support or maintenance of a person whom the holder is obligated to support, except to the extent the income is so applied. See paragraphs (a), (b), and (c) of §...

  12. C1q Nephropathy: The Unique Underrecognized Pathological Entity

    PubMed Central

    Devasahayam, Joe; Erode-Singaravelu, Gowrishankar; Bhat, Zeenat; Oliver, Tony; Chandran, Arul; Zeng, Xu; Dakshinesh, Paramesh; Pillai, Unni

    2015-01-01

    C1q nephropathy is a rare glomerular disease with characteristic mesangial C1q deposition noted on immunofluorescence microscopy. It is histologically defined and poorly understood. Light microscopic features are heterogeneous and comprise minimal change disease (MCD), focal segmental glomerulosclerosis (FSGS), and proliferative glomerulonephritis. Clinical presentation is also diverse, and ranges from asymptomatic hematuria or proteinuria to frank nephritic or nephrotic syndrome in both children and adults. Hypertension and renal insufficiency at the time of diagnosis are common findings. Optimal treatment is not clear and is usually guided by the underlying light microscopic lesion. Corticosteroids are the mainstay of treatment, with immunosuppressive agents reserved for steroid resistant cases. The presence of nephrotic syndrome and FSGS appear to predict adverse outcomes as opposed to favorable outcomes in those with MCD. Further research is needed to establish C1q nephropathy as a universally recognized distinct clinical entity. In this paper, we discuss the current understanding of pathogenesis, histopathology, clinical features, therapeutic options, and outcomes of C1q nephropathy. PMID:26640759

  13. Participatory Training to Improve Safety and Health in Small Construction Sites in Some Countries in Asia: Development and Application of the WISCON Training Program.

    PubMed

    Kawakami, Tsuyoshi

    2016-08-01

    A participatory training program, Work Improvement in Small Construction Sites, was developed to provide practical support measures to the small construction sector. Managers and workers from selected small sites were interviewed about their occupational safety and health risks. The Work Improvement in Small Construction Sites training program comprised a 45-item action checklist, photos, and illustrations showing local examples and group work methods. Pilot training workshops were carried out with workers and employers in Cambodia, Laos, Mongolia, Thailand, and Vietnam. Participants subsequently planned, and using locally available low-cost materials, implemented their own improvements such as hand-made hand trucks to carry heavy materials, removal of projecting nails from timber materials, and fences to protect roof workers from falling. Local Work Improvement in Small Construction Sites trainers consisting of government officials, workers, employers, and nongovernment organization representatives were then trained to implement the Work Improvement in Small Construction Sites training widely. Keys to success were easy-to-apply training tools aiming at immediate, low-cost improvements, and collaboration with various local people's networks. PMID:27296976

  14. Participatory Training to Improve Safety and Health in Small Construction Sites in Some Countries in Asia: Development and Application of the WISCON Training Program.

    PubMed

    Kawakami, Tsuyoshi

    2016-08-01

    A participatory training program, Work Improvement in Small Construction Sites, was developed to provide practical support measures to the small construction sector. Managers and workers from selected small sites were interviewed about their occupational safety and health risks. The Work Improvement in Small Construction Sites training program comprised a 45-item action checklist, photos, and illustrations showing local examples and group work methods. Pilot training workshops were carried out with workers and employers in Cambodia, Laos, Mongolia, Thailand, and Vietnam. Participants subsequently planned, and using locally available low-cost materials, implemented their own improvements such as hand-made hand trucks to carry heavy materials, removal of projecting nails from timber materials, and fences to protect roof workers from falling. Local Work Improvement in Small Construction Sites trainers consisting of government officials, workers, employers, and nongovernment organization representatives were then trained to implement the Work Improvement in Small Construction Sites training widely. Keys to success were easy-to-apply training tools aiming at immediate, low-cost improvements, and collaboration with various local people's networks.

  15. Isolation and mode of action of bacteriocin BacC1 produced by nonpathogenic Enterococcus faecium C1.

    PubMed

    Goh, H F; Philip, K

    2015-08-01

    Lactic acid bacteria are present in fermented food products and help to improve shelf life and enhance the flavor of the food. They also produce metabolites such as bacteriocins to prevent the growth of undesirable or pathogenic bacteria. In this study, Enterococcus faecium C1 isolated from fermented cow milk was able to produce bacteriocin BacC1 and inhibit the growth of selected food-spoilage bacteria. The bacteriocin was purified through 4 steps: ammonium sulfate precipitation, hydrophobic interaction column, a series of centrifugal steps, and finally reversed-phase HPLC. A membrane permeability test using SYTOX green dye (Invitrogen, Grand Island, NY) showed that the bacteriocin caused significant disruptions to the test bacterial membrane, as shown by transmission electron microscopy. The molecular weight of the BacC1 obtained from SDS-PAGE was around 10kDa, and N-terminal sequencing revealed a partial amino acid sequence of BacC1: GPXGPXGP. The bacterial strain was nonhemolytic and not antibiotic resistant. Therefore, it has high potential for application in the food industry as an antimicrobial agent to extend the shelf life of food products.

  16. Isolation and mode of action of bacteriocin BacC1 produced by nonpathogenic Enterococcus faecium C1.

    PubMed

    Goh, H F; Philip, K

    2015-08-01

    Lactic acid bacteria are present in fermented food products and help to improve shelf life and enhance the flavor of the food. They also produce metabolites such as bacteriocins to prevent the growth of undesirable or pathogenic bacteria. In this study, Enterococcus faecium C1 isolated from fermented cow milk was able to produce bacteriocin BacC1 and inhibit the growth of selected food-spoilage bacteria. The bacteriocin was purified through 4 steps: ammonium sulfate precipitation, hydrophobic interaction column, a series of centrifugal steps, and finally reversed-phase HPLC. A membrane permeability test using SYTOX green dye (Invitrogen, Grand Island, NY) showed that the bacteriocin caused significant disruptions to the test bacterial membrane, as shown by transmission electron microscopy. The molecular weight of the BacC1 obtained from SDS-PAGE was around 10kDa, and N-terminal sequencing revealed a partial amino acid sequence of BacC1: GPXGPXGP. The bacterial strain was nonhemolytic and not antibiotic resistant. Therefore, it has high potential for application in the food industry as an antimicrobial agent to extend the shelf life of food products. PMID:26004828

  17. The effect of excision of the posterior arch of C1 on C1/C2 fusion using transarticular screws.

    PubMed

    Chang, K C; Samartzis, D; Fuego, S M; Dhatt, S S; Wong, Y W; Cheung, W Y; Luk, K D K; Cheung, K M C

    2013-07-01

    Transarticular screw fixation with autograft is an established procedure for the surgical treatment of atlantoaxial instability. Removal of the posterior arch of C1 may affect the rate of fusion. This study assessed the rate of atlantoaxial fusion using transarticular screws with or without removal of the posterior arch of C1. We reviewed 30 consecutive patients who underwent atlantoaxial fusion with a minimum follow-up of two years. In 25 patients (group A) the posterior arch of C1 was not excised (group A) and in five it was (group B). Fusion was assessed on static and dynamic radiographs. In selected patients CT imaging was also used to assess fusion and the position of the screws. There were 15 men and 15 women with a mean age of 51.2 years (23 to 77) and a mean follow-up of 7.7 years (2 to 11.6). Stable union with a solid fusion or a stable fibrous union was achieved in 29 patients (97%). In Group A, 20 patients (80%) achieved a solid fusion, four (16%) a stable fibrous union and one (4%) a nonunion. In Group B, stable union was achieved in all patients, three having a solid fusion and two a stable fibrous union. There was no statistically significant difference between the status of fusion in the two groups. Complications were noted in 12 patients (40%); these were mainly related to the screws, and included malpositioning and breakage. The presence of an intact or removed posterior arch of C1 did not affect the rate of fusion in patients with atlantoaxial instability undergoing C1/C2 fusion using transarticular screws and autograft.

  18. Using Scratch with Primary School Children: An Evaluation of Games Constructed to Gauge Understanding of Programming Concepts

    ERIC Educational Resources Information Center

    Wilson, Amanda; Hainey, Thomas; Connolly, Thomas M.

    2013-01-01

    Newer approaches such as games-based learning (GBL) and games-based construction are being adopted to motivate and engage students within the Curriculum for Excellence (CfE) in Scotland. GBL and games-based construction suffer from a dearth of empirical evidence supporting their validity as teaching and learning approaches. To address this issue…

  19. Project Build: Integrating Technical and Employability Skills in a Construction Industry-Based Welfare-to-Work Training Program.

    ERIC Educational Resources Information Center

    Overtoom, Christine G.

    Project Build is an eight-week course in construction industry basics for unemployed residents of the neighborhoods surrounding The Ohio State University. Developing curriculum for Project Build revealed that existing training materials in the construction industry focus on technical skills. Extensive conversations with industry experts about…

  20. The C1s core line in irradiated graphite

    SciTech Connect

    Speranza, Giorgio; Minati, Luca; Anderle, Mariano

    2007-08-15

    Recently, plasma deposited amorphous carbon films have been the subject of extensive experimental and theoretical investigations aimed at correlating their electronic, structural, and mechanical properties to growth parameters. To investigate these properties, different spectral parameters reflecting the electronic structure of carbon-based materials are proposed in literature. The effects of various electronic configurations on the carbon photoelectron spectra are analyzed here with particular attention to C1s core line with the aim to better interpret its structure. The latter is commonly fitted under the assumption that it can be described by using just two spectral components related to sp{sup 2} and sp{sup 3} hybrids. Their relative intensities are then used to estimate the sp{sup 2} and sp{sup 3} phases. We show that, in the presence of an amorphous network, the C1s line shape is the result of a more complex mixture of electronic states. Ar{sup +} irradiated graphite and successive oxidation was used to identify spectral features to better describe the C1s line shape.

  1. Immune complex processing in C1q-deficient mice.

    PubMed

    Nash, J T; Taylor, P R; Botto, M; Norsworthy, P J; Davies, K A; Walport, M J

    2001-02-01

    Complement and Fcgamma receptors are known to mediate the processing of immune complexes (IC), and abnormalities in these mechanisms may predispose to the development of lupus. We explored the processing of IC in mice deficient in complement component C1q. 125I-labelled IC comprising Hepatitis B surface antigen (HBsAg)/human anti-HBsAg (HBsAg/Ab) were injected intravenously and the sites of IC clearance determined by direct counting of organ uptake at various time points. The liver and spleen were the main sites of IC uptake in all mice. The splenic uptake of IC was significantly reduced in the C1q-deficient mice compared with the control mice. C1q-deficient mice also exhibited an initial accelerated hepatic uptake of IC similar to that seen in human subjects with hypocomplementaemia. The hepatic localization of IC at later time points was similar in both groups of mice. These data in mice are consistent with previous observations in humans that confirm that the classical pathway of complement plays an important role in the appropriate processing of IC in vivo.

  2. Logan Library, Final Performance Report for Library Services and Construction Act (LSCA) Title VI, Library Literacy Program.

    ERIC Educational Resources Information Center

    Steinhoff, Nadene; Jenkins, Ronald

    The Bridgerland Literacy program of Logan Library (Logan, Utah) involved recruitment, retention, public awareness, training, basic literacy, collection development, tutoring, computer assisted, employment oriented, intergenerational/family, and English as a Second Language (ESL) programs. The program served a community of 50,000-100,000 people,…

  3. C1 CHEMISTRY FOR THE PRODUCTION OF ULTRA-CLEAN LIQUID TRANSPORTATION FUELS AND HYDROGEN

    SciTech Connect

    Gerald P. Huffman

    2003-03-31

    Faculty and students from five universities--the University of Kentucky, University of Pittsburgh, University of Utah, West Virginia University, and Auburn University--are collaborating in a research program to develop C1 chemistry processes to produce ultra-clean liquid transportation fuels and hydrogen, the zero-emissions transportation fuel of the future. The feedstocks contain one carbon atom per molecular unit. They include synthesis gas (syngas), a mixture of carbon monoxide and hydrogen produced by coal gasification or reforming of natural gas, methane, methanol, carbon dioxide, and carbon monoxide. An important objective is to develop C1 technology for the production of transportation fuel from domestically plentiful resources such as coal, coalbed methane, and natural gas. An Industrial Advisory Board with representatives from Chevron-Texaco, Eastman Chemical, Conoco-Phillips, Energy International, the Department of Defense, and Tier Associates provides guidance on the practicality of the research.

  4. The distortive mechanism for the activation of complement component C1 supported by studies with a monoclonal antibody against the "arms" of C1q.

    PubMed

    Hoekzema, R; Martens, M; Brouwer, M C; Hack, C E

    1988-05-01

    A mouse monoclonal antibody (IgG1 isotype) against human C1q (MAb 130) is presented that activates C1 in serum through its antigen-binding sites at an optimal molar ratio of 3 MAbs:1 C1q. The antibody does not inhibit binding of C1q to IgG. Experiments with pepsin- and collagenase-digested C1q showed that MAb 130 binds to the fibril-like strands (arms) of C1q, close to the globular heads. Bivalency of MAb 130 was a requirement for C1-activation, but not for binding to C1q. Increasing the segmental flexibility of the intact antibody by reduction and alkylation destroyed its capacity to activate C1. A MAb against the globular heads of C1q completely inhibited C1-activation by aggregated IgG (AHG), but did not prevent activation by MAb 130. C1, reconstituted by adding C1q-stalks that lack the globular heads to C1q-depleted serum was not activated by AHG, whereas activation by MAb 130 was not affected. Activation of serum-C1 by AHG and MAb 130 was inhibited by addition of excess purified C1-inhibitor in a comparable and dose-dependent manner. Sucrose-gradient analysis indicated a predominance of stable complexes of a single C1q-molecule with three MAbs at the optimal activating ratio. When isolated and added to C1q-depleted serum, these complexes activated C1 efficiently. A mechanism for activation by MAb 130 is proposed that supports the "distortive" model of C1-activation.

  5. Phase equilibria of NdC1 3NaClKCl

    NASA Astrophysics Data System (ADS)

    Hosoya, Yuji; Terai, Takayuki; Tanaka, Satoru; Takahashi, Yoichi

    1997-08-01

    Molten chloride is considered to be applied to a fast-breeder-reactor fuel and a solvent in the pyrochemical reprocessing of spent nuclear fuel. In this work, phase diagrams for molten chloride systems were constructed, using NdCl 3 as an imitative substance in place of UCl 3 or PuCl 3. A compound of 3NdC1 3 · NaCl, which melts incongruently at 540°C to NdCl 3 and liquid and a eutectic at 437°C were found in the NdC1 3NaCl system. In the NdCl 3KCl system, many invariant reactions were observed: the decomposition of NdCl 3 · 2KCl and 2NdCl 3 · KCl at 444 and 474°C, respectively; a eutectic at 489°C; a peritectic at 506°C and a monotectic at 624°C. It is thought that there should be a peritectic compound of 6NdCl 3 · KCl above 474°C. A compound of 2NdC1 3 · NaCl · KCl was considered to exist in the ternary system of NdCl 3NaClKCl, to which attention should be paid in determining the composition of the fuel of the molten-salt fast breeder reactor.

  6. Workshops and incentive loans program for construction of solar greenhouses, grain dryers, window boxes and food dryers. Final performance report

    SciTech Connect

    Walker, D.H.

    1982-02-01

    A Solar Loan Fund was established to provide low-interest incentive loans to families desiring to construct their own solar devices, the only stipulations being allowance of reasonable public visitation and monitoring and employment of parish-trained construction teams if labor is to be hired. About 30 vocational agriculture students built window box collector units as part of their classroom experience, trying several designs. Later, 12 to 15 families built window box collectors for their use. A week long Energy Responsibility: Today and Tomorrow workshop was held involving construction projects and mini-seminars. Another workshop was held to construct bread box type solar hot water heaters, and yet another to build solar food dryers. (LEW)

  7. Lack of activation of C1, despite circulating immune complexes detected by two C1q methods, in patients with rheumatoid arthritis.

    PubMed

    Hack, C E; Eerenberg-Belmer, A J; Lim, U G; Haverman, J; Aalberse, R C

    1984-01-01

    The activation of C1 by circulating immune complexes in patients with rheumatoid arthritis was investigated. C1rC1s(C1-In)2 complexes in EDTA-plasma, reflecting C1 activation in vivo, were slightly raised in 35 of 57 patients with rheumatoid arthritis, though most patients had elevated levels of circulating immune complexes as measured with either the 125I-C1q binding test or the C1q solid phase assay. The activation of C1 by circulating immune complexes in vitro was investigated by measuring the generation of C1rC1s(C1-In)2 complexes during 60 minutes at 37 degrees C in diluted recalcified EDTA-plasma. In 16 of the 57 patients, a slightly increased C1 activation in vitro was observed. These patients tended to have high levels of circulating immune complexes. However, the majority of the patients with high levels of circulating immune complexes showed a normal C1 activation in vitro. Therefore, it was concluded that measurement of circulating immune complexes by either of the two C1q methods in patients with rheumatoid arthritis does not imply that these circulating immune complexes are able to activate C1.

  8. Rapid Reactivation of Deep Subsurface Microbes in the Presence of C-1 Compounds.

    PubMed

    Rajala, Pauliina; Bomberg, Malin; Kietäväinen, Riikka; Kukkonen, Ilmo; Ahonen, Lasse; Nyyssönen, Mari; Itävaara, Merja

    2015-02-05

    Microorganisms in the deep biosphere are believed to conduct little metabolic activity due to low nutrient availability in these environments. However, destructive penetration to long-isolated bedrock environments during construction of underground waste repositories can lead to increased nutrient availability and potentially affect the long-term stability of the repository systems, Here, we studied how microorganisms present in fracture fluid from a depth of 500 m in Outokumpu, Finland, respond to simple carbon compounds (C-1 compounds) in the presence or absence of sulphate as an electron acceptor. C-1 compounds such as methane and methanol are important intermediates in the deep subsurface carbon cycle, and electron acceptors such as sulphate are critical components of oxidation processes. Fracture fluid samples were incubated in vitro with either methane or methanol in the presence or absence of sulphate as an electron acceptor. Metabolic response was measured by staining the microbial cells with fluorescent dyes that indicate metabolic activity and transcriptional response with RT-qPCR. Our results show that deep subsurface microbes exist in dormant states but rapidly reactivate their transcription and respiration systems in the presence of C-1 substrates, particularly methane. Microbial activity was further enhanced by the addition of sulphate as an electron acceptor. Sulphate- and nitrate-reducing microbes were particularly responsive to the addition of C-1 compounds and sulphate. These taxa are common in deep biosphere environments and may be affected by conditions disturbed by bedrock intrusion, as from drilling and excavation for long-term storage of hazardous waste.

  9. Rapid Reactivation of Deep Subsurface Microbes in the Presence of C-1 Compounds

    PubMed Central

    Rajala, Pauliina; Bomberg, Malin; Kietäväinen, Riikka; Kukkonen, Ilmo; Ahonen, Lasse; Nyyssönen, Mari; Itävaara, Merja

    2015-01-01

    Microorganisms in the deep biosphere are believed to conduct little metabolic activity due to low nutrient availability in these environments. However, destructive penetration to long-isolated bedrock environments during construction of underground waste repositories can lead to increased nutrient availability and potentially affect the long-term stability of the repository systems, Here, we studied how microorganisms present in fracture fluid from a depth of 500 m in Outokumpu, Finland, respond to simple carbon compounds (C-1 compounds) in the presence or absence of sulphate as an electron acceptor. C-1 compounds such as methane and methanol are important intermediates in the deep subsurface carbon cycle, and electron acceptors such as sulphate are critical components of oxidation processes. Fracture fluid samples were incubated in vitro with either methane or methanol in the presence or absence of sulphate as an electron acceptor. Metabolic response was measured by staining the microbial cells with fluorescent dyes that indicate metabolic activity and transcriptional response with RT-qPCR. Our results show that deep subsurface microbes exist in dormant states but rapidly reactivate their transcription and respiration systems in the presence of C-1 substrates, particularly methane. Microbial activity was further enhanced by the addition of sulphate as an electron acceptor. Sulphate- and nitrate-reducing microbes were particularly responsive to the addition of C-1 compounds and sulphate. These taxa are common in deep biosphere environments and may be affected by conditions disturbed by bedrock intrusion, as from drilling and excavation for long-term storage of hazardous waste.

  10. Rapid Reactivation of Deep Subsurface Microbes in the Presence of C-1 Compounds

    PubMed Central

    Rajala, Pauliina; Bomberg, Malin; Kietäväinen, Riikka; Kukkonen, Ilmo; Ahonen, Lasse; Nyyssönen, Mari; Itävaara, Merja

    2015-01-01

    Microorganisms in the deep biosphere are believed to conduct little metabolic activity due to low nutrient availability in these environments. However, destructive penetration to long-isolated bedrock environments during construction of underground waste repositories can lead to increased nutrient availability and potentially affect the long-term stability of the repository systems, Here, we studied how microorganisms present in fracture fluid from a depth of 500 m in Outokumpu, Finland, respond to simple carbon compounds (C-1 compounds) in the presence or absence of sulphate as an electron acceptor. C-1 compounds such as methane and methanol are important intermediates in the deep subsurface carbon cycle, and electron acceptors such as sulphate are critical components of oxidation processes. Fracture fluid samples were incubated in vitro with either methane or methanol in the presence or absence of sulphate as an electron acceptor. Metabolic response was measured by staining the microbial cells with fluorescent dyes that indicate metabolic activity and transcriptional response with RT-qPCR. Our results show that deep subsurface microbes exist in dormant states but rapidly reactivate their transcription and respiration systems in the presence of C-1 substrates, particularly methane. Microbial activity was further enhanced by the addition of sulphate as an electron acceptor. Sulphate- and nitrate-reducing microbes were particularly responsive to the addition of C-1 compounds and sulphate. These taxa are common in deep biosphere environments and may be affected by conditions disturbed by bedrock intrusion, as from drilling and excavation for long-term storage of hazardous waste. PMID:27682076

  11. The Social Construction of "Evidence-Based" Drug Prevention Programs: A Reanalysis of Data from the Drug Abuse Resistance Education (DARE) Program

    ERIC Educational Resources Information Center

    Gorman, Dennis M.; Huber, J. Charles, Jr.

    2009-01-01

    This study explores the possibility that any drug prevention program might be considered "evidence-based" given the use of data analysis procedures that optimize the chance of producing statistically significant results by reanalyzing data from a Drug Abuse Resistance Education (DARE) program evaluation. The analysis produced a number of…

  12. Reduction of CO2 to C1 products and fuel

    USGS Publications Warehouse

    Mill, T.; Ross, D.

    2002-01-01

    Photochemical semiconductor processes readily reduced CO2 to a broad range of C1 products. However the intrinsic and solar efficiencies for the processes were low. Improved quantum efficiencies could be realized utilizing quantum-sized particles, but at the expense of using less of the visible solar spectrum. Conversely, semiconductors with small bandgaps used more of the visible solar spectrum at the expense of quantum efficiency. Thermal reduction of CO2 with Fe(II) was thermodynamically favored for forming many kinds of organic compounds and occurred readily with olivine and other Fe(II) minerals above 200??C to form higher alkanes and alkenes. No added hydrogen was required.

  13. "You Get to Be Yourself": Visual Arts Programs, Identity Construction and Learners of English as an Additional Language

    ERIC Educational Resources Information Center

    Wielgosz, Meg; Molyneux, Paul

    2015-01-01

    Students learning English as an additional language (EAL) in Australian schools frequently struggle with the cultural and linguistic demands of the classroom while concurrently grappling with issues of identity and belonging. This article reports on an investigation of the role primary school visual arts programs, distinct programs with a…

  14. Santa Clara County Library, Final Performance Report for Library Services and Construction Act (LSCA) Title VI, Library Literacy Program.

    ERIC Educational Resources Information Center

    Willingham, Taylor; Trajico, Charity A.; Levy, Michael

    This final performance report provides project outcome information and data to the U.S. Department of Education for the federally-funded Library Literacy Program. The Santa Clara County (California) Library Reading Program Computer Aided Literacy (CAL) Project involved recruitment, public awareness, training, basic literacy, collection…

  15. Lowell Public Library, Final Performance Report for Library Services and Construction Act (LSCA) Title VI, Library Literacy Program.

    ERIC Educational Resources Information Center

    Maravilla, Virginia

    The Lowell Public Library (Indiana) Adult Literacy Program expanded literacy efforts of the library and its volunteer tutors by increasing the program enrollment numbers of the functionally illiterate English-speaking, English as a Second Language (ESL), migrant workers, and Basic Math students; assisted students in achieving their stated goals in…

  16. Bayard Taylor Memorial Library, Final Performance Report for Library Services and Construction Act (LSCA) Title VI, Library Literacy Program.

    ERIC Educational Resources Information Center

    Peters, Alice L.; Calio, Susan

    This final performance report provides project outcome information and data to the U.S. Department of Education for the federally-funded Library Literacy Program. The Bayard Taylor Memorial Library (Kennett Square, Pennsylvania) conducted the Outreach Project, a project that involved public awareness, training, rural oriented programs, basic…

  17. Creating an Anti-Bullying Culture in Secondary Schools: Characteristics to Consider When Constructing Appropriate Anti-Bullying Programs

    ERIC Educational Resources Information Center

    Jones, Joseph R.; Augustine, Sharon Murphy

    2015-01-01

    Bullying in schools is a tremendous challenge that many secondary educators are attempting to address within their school environments. However, educators are often unsure of the attributes of an effective anti-bullying program; thus, they tend to create programs on a "trial and error" basis. This article provides an overview of the…

  18. Blanchard Community Library, Final Performance Report for Library Services and Construction Act (LSCA) Title VI, Library Literacy Program.

    ERIC Educational Resources Information Center

    Hunt, Elaine

    The Family Literacy: Aid in Reading (FLAIR) program was a project of the Blanchard Community Library (Santa Paula, California), conducted during fiscal year 1992-93. The project involved recruitment, retention, public awareness, basic literacy, tutoring, computer assisted, employment oriented, and English as a Second Language (ESL) programs. The…

  19. Alameda County Library Workplace Literacy, Final Performance Report for Library Services and Construction Act (LSCA) Title VI, Library Literacy Program.

    ERIC Educational Resources Information Center

    Drobner, Sherry

    The Adult Literacy Program at the Alameda County Library provided employment oriented programs. The project served a community of over 200,000 people, and targeted workforce/workplace learners. The project served 578 adult learners and provided 35,904 hours of direct tutoring service. The report provides a comparison of actual accomplishments to…

  20. A job safety program for construction workers designed to reduce the potential for occupational injury using tool box training sessions and computer-assisted biofeedback stress management techniques.

    PubMed

    Johnson, Kenneth A; Ruppe, Joan

    2002-01-01

    This project was conducted with a multicultural construction company in Hawaii, USA. The job duties performed included drywall and carpentry work. The following objectives were selected for this project: (a) fire prevention training and inspection of first aid equipment; (b) blood-borne pathogen training and risk evaluation; (c) ergonomic and risk evaluation intervention program; (d) electrical safety training and inspection program; (e) slips, trips, and falls safety training; (f) stress assessment and Personal Profile System; (g) safety and health program survey; (h) improving employee relations and morale by emphasizing spirituality; and (i) computer-assisted biofeedback stress management training. Results of the project indicated that observed safety hazards, reported injuries, and levels of perceived stress. were reduced for the majority of the population. PMID:12189103

  1. C1 inhibitor deficiency: 2014 United Kingdom consensus document.

    PubMed

    Longhurst, H J; Tarzi, M D; Ashworth, F; Bethune, C; Cale, C; Dempster, J; Gompels, M; Jolles, S; Seneviratne, S; Symons, C; Price, A; Edgar, D

    2015-06-01

    C1 inhibitor deficiency is a rare disorder manifesting with recurrent attacks of disabling and potentially life-threatening angioedema. Here we present an updated 2014 United Kingdom consensus document for the management of C1 inhibitor-deficient patients, representing a joint venture between the United Kingdom Primary Immunodeficiency Network and Hereditary Angioedema UK. To develop the consensus, we assembled a multi-disciplinary steering group of clinicians, nurses and a patient representative. This steering group first met in 2012, developing a total of 48 recommendations across 11 themes. The statements were distributed to relevant clinicians and a representative group of patients to be scored for agreement on a Likert scale. All 48 statements achieved a high degree of consensus, indicating strong alignment of opinion. The recommendations have evolved significantly since the 2005 document, with particularly notable developments including an improved evidence base to guide dosing and indications for acute treatment, greater emphasis on home therapy for acute attacks and a strong focus on service organization. PMID:25605519

  2. Construction and bootstrap analysis of DNA fingerprinting-based phylogenetic trees with the freeware program FreeTree: application to trichomonad parasites.

    PubMed

    Hampl, V; Pavlícek, A; Flegr, J

    2001-05-01

    The Win95/98/NT program FreeTree for computation of distance matrices and construction of phylogenetic or phenetic trees on the basis of random amplified polymorphic DNA (RAPD), RFLP and allozyme data is presented. In contrast to other similar software, the program FreeTree (available at http://www.natur.cuni.cz/~flegr/programs/freetree or http://ijs.sgmjournals.org/content/vol51/issue3/) can also assess the robustness of the tree topology by bootstrap, jackknife or operational taxonomic unit-jackknife analysis. Moreover, the program can be also used for the analysis of data obtained in several independent experiments performed with non-identical subsets of taxa. The function of the program was demonstrated by an analysis of RAPD data from 42 strains of 10 species of trichomonads. On the phylogenetic tree constructed using FreeTree, the high bootstrap values and short terminal branches for the Tritrichomonas foetus/suis 14-strain branch suggested relatively recent and probably clonal radiation of this species. At the same time, the relatively lower bootstrap values and long terminal branches for the Trichomonas vaginalis 20-strain branch suggested more ancient radiation of this species and the possible existence of genetic recombination (sexual reproduction) in this human pathogen. The low bootstrap values and the star-like topology of the whole Trichomonadidae tree confirm that the RAPD method is not suitable for phylogenetic analysis of protozoa at the level of higher taxa. It is proposed that the repeated bootstrap analysis should be an obligatory part of any RAPD study. It makes it possible to assess the reliability of the tree obtained and to adjust the amount of collected data (the number of random primers) to the amount of phylogenetic signals in the RAPD data of the taxon analysed. The FreeTree program makes such analysis possible. PMID:11411692

  3. Polyethylene glycol enhances the binding of C1q to circulating immune complexes.

    PubMed

    Hack, C E; Eerenberg-Belmer, A J; Hannema, A J; Out, T A; Aalberse, R C

    1981-01-01

    By radioimmunoassay we measured the amount of endogenous C1q that was precipitated by polyethylene glycol (PEG) under the conditions of the 125I-C1q-binding test (C1q-BT). We found a linear correlation between the percentage endogenous C1q that was precipitated and the 125I-C1q-binding activity (C1q-BA). We concluded that the 125I-C1q behaves like the endogenous C1q. To detect circulating immune complexes (CIC) which had already bound C1q, human sera were added to tubes coated with anti-C1q. Under the conditions used, no C1q-bearing CIC were detected. In addition, 7 sera from patients with high C1q-BA were analyzed by sucrose-gradient ultracentrifugation. No C1q was found in the fast sedimenting fractions, although C1q-BA was detected in these fractions. With IgG-coated tubes we observed that PEG enhanced the binding of 125I-C1q as well as endogenous C1q to aggregated and monomeric IgG. PEG also enhanced the binding of CIC to C1q-coated tubes. The results suggest that CIC detected by the C1q-BT do not bear C1q in significant amounts in the circulation and that these CIC become detectable only in the presence of PEG.

  4. Review of Programs: Architecture; Architectural Technology; Landscape Architecture; Interior Design; Construction and Construction Technology; Building Construction; Urban and Regional Planning. Report to the Board of Regents, State University System of Florida.

    ERIC Educational Resources Information Center

    McMinn, William G.

    This report is an update of a report on the development and status of various programs in architecture and related fields in the State University System of Florida, a report that was submitted to the Board of Regents in May 1983. The objectives of this updated report, like those of the earlier one, are to review the anticipated needs of the…

  5. Determining the locations of the various CIRC recording format information blocks (user data blocks, C2 and C1 words and EFM frames) on a recorded compact disc

    NASA Technical Reports Server (NTRS)

    Howe, Dennis G.

    1993-01-01

    Just prior to its being EFM modulated (i.e., converted to eight-to-fourteen channel data by the EFM encoder) and written to a Compact Disc (CD), information that passes through the CIRC Block Encoder is grouped into 33-byte blocks referred to as EFM frames. Twenty four of the bytes that make up a given EFM frame are user data that was input into the CIRC encoder at various (different) times, 4 of the bytes of this same EFM frame were created by the C2 ECC encoder (each at a different time), and another 4 were created by the C1 ECC encoder (again, each at a different time). The one remaining byte of the given EFM frame, which is known as the EFM frame C&D (for Control & Display) byte, carries information that identifies which portion of the current disc program track the given EFM frame belongs to and also specifies the location of the given EFM frame on the disc (in terms of a time stamp that has a resolution of l/75th second, or 98 EFM frames). (Note: since the program track and time information is stored as a 98-byte word, a logical group consisting of 98 consecutive EFM frames must be read, and their respective C&D bytes must be catenated and decoded, before the program track identification and time position information that pertains to the entire block of 98 EFM frames can be obtained.) The C&D byte is put at the start (0th byte) of an EFM frame in real time; its placement completes the construction of the EFM frame - it is assigned just before the EFM frame enters the EFM encoder. Four distinct blocks of data are referred to: 24-byte User Input Data Blocks; 28-byte C2 words; 32-byte C1 words; and 33-byte EFM frames.

  6. Architectural and Building Construction Technology; A Suggested 2-Year Post High School Curriculum. Technical Education Program Series No. 9.

    ERIC Educational Resources Information Center

    Office of Education (DHEW), Washington, DC.

    This curriculum guide is for administrators and their advisors to use in meeting local, state, and regional needs in training architectual and building construction technicians at the post-high school level. It was developed by a technical education specialist at the national level. The guide provides: (1) a suggested curriculum plan, (2) course…

  7. Encouraging PV Adoption in New Market-Rate ResidentialConstruction: A Critical Review of Program Experiences to Date

    SciTech Connect

    Barbose, Galen; Wiser, Ryan; Bolinger, Mark

    2006-04-24

    In this paper, we review experiences with programs to support the deployment of photovoltaics (PV) in new, market-rate homes, drawing upon interviews with program managers around the country, project data, and publicly-available documentation on program design, impacts, and experiences. We focus on state clean energy funds, which have been established in 14 U.S. states to build markets for clean energy resources, as well as a select number of other state or local organizations whose activities are particularly noteworthy. We describe the types of programs implemented and their impacts to date, and discuss key issues and lessons learned for initiatives aimed at growing the new home market for PV.

  8. Programs.

    ERIC Educational Resources Information Center

    Community College Journal, 1996

    1996-01-01

    Includes a collection of eight short articles describing model community college programs. Discusses a literacy program, a mobile computer classroom, a support program for at-risk students, a timber-harvesting program, a multimedia presentation on successful women graduates, a career center, a collaboration with NASA, and an Israeli engineering…

  9. A review of the constructs, curriculum and training data from a workforce development program for recovery support specialists.

    PubMed

    Stoneking, Beth C; McGuffin, Beverly A

    2007-01-01

    The theoretical underpinnings, training content and initial training data of a workforce development program are described. The program was designed for people with psychiatric and/or substance use disorders, and provides skills to improve their employability in the behavioral health system. Trainees rated their knowledge, skills and attitudes as improved after training. Supervisor ratings of participants after three months of employment are consistent with the participant's self-ratings.

  10. Microbial growth on C1 compounds. Incorporation of C1 units into allulose phosphate by extracts of Pseudomonas methanica

    PubMed Central

    Kemp, M. B.; Quayle, J. R.

    1966-01-01

    1. Incubation of cell-free extracts of methane- or methanol-grown Pseudomonas methanica with [14C]formaldehyde and d-ribose 5-phosphate leads to incorporation of radioactivity into a non-volatile product, which has the chromatographic properties of a phosphorylated compound. 2. Treatment of this reaction product with a phosphatase, followed by chromatography, shows the presence of two compounds whose chromatographic properties are consistent with their being free sugars. 3. The minor component of the dephosphorylated products has been identified as fructose. The major component has been identified as allulose (psicose) on the basis of co-chromatography, co-crystallization of the derived phenylosazone and dinitrophenylosazone with authentic derivatives of allulose and behaviour towards oxidation with bromine water. 4. It is suggested that the bacterial extracts catalyse the condensation of a C1 unit identical with, or derived from, formaldehyde with ribose 5-phosphate to give allulose 6-phosphate. 5. Testing of hexose phosphates and pentose phosphates as substrates has so far shown the reaction to be specific for ribose 5-phosphate. 6. The condensation reaction is not catalysed by extracts of methanol-grown Pseudomonas AM1. 7. A variant of the pentose phosphate cycle, involving this condensation reaction, is suggested as an explanation for the net synthesis of C3 compounds from C1 units by P. methanica. PMID:5965346

  11. [C1Q NEPHROPATHY: CASE REPORTS AND LITERATURE REVIEW].

    PubMed

    Galešić, Krešimir; Horvatić, Ivica; Batinić, Danica; Milošević, Danko; Saraga, Marijan; Durdov, Merica Glavina; Ljubanović, Danica Galešić

    2015-01-01

    C1q nephropathy is considered a form of glomerulonephritis, defined by histological findings of dominant Clq immune deposits in renal biopsy. It is a rare disease, most often manifested in children and young adults. The most common clinical manifestation of the disease is nephrotic syndrome, but other renal syndromes could also be found. The cause of the disease is not known, but the immune pathogenesis could be assumed. Often, resistance to glucocorticoid or other immunosuppressive therapy is present, potentially leading to chronic renal insufficiency. We present ten patients with renal biopsy and clinical findings of Clq nephropathy. None of the patients had clinical or serological manifestations of systemic lupus. All patients had normal findings of C3 and C4 components of complement, as well as normal ANF, anti-dsD-NA and ANCA antibodies. PMID:26749950

  12. A Selection of Recent Advances in C1 Chemistry.

    PubMed

    Mesters, Carl

    2016-06-01

    This review presents a selection of recent publications related to the chemistry and catalysis of C1 molecules, including methane, methanol, carbon monoxide, and carbon dioxide. These molecules play an important role in the current supply of energy and chemicals and will likely become even more relevant because of the need to decarbonize fuels (shift from coal to natural gas) in line with CO2 capture and use to mitigate global warming, as well as a gradual shift on the supply side from crude oil to natural gas. This review includes both recent industrial developments, such as the huge increase in methanol-to-olefins-capacity build in China and the demonstration of oxidative coupling of methane, and scientific developments in these chemistries facilitated by improved capabilities in, for example, analytical tools and computational modeling.

  13. Plasma enhanced C1 chemistry for green technology

    NASA Astrophysics Data System (ADS)

    Nozaki, Tomohiro

    2013-09-01

    Plasma catalysis is one of the innovative next generation green technologies that meet the needs for energy and materials conservation as well as environmental protection. Non-thermal plasma uniquely generates reactive species independently of reaction temperature, and these species are used to initiate chemical reactions at unexpectedly lower temperatures than normal thermochemical reactions. Non-thermal plasma thus broadens the operation window of existing chemical conversion processes, and ultimately allows modification of the process parameters to minimize energy and material consumption. We have been specifically focusing on dielectric barrier discharge (DBD) as one of the viable non-thermal plasma sources for practical fuel reforming. In the presentation, room temperature one-step conversion of methane to methanol and hydrogen using a miniaturized DBD reactor (microplasma reactor) is highlighted. The practical impact of plasma technology on existing C1-chemistry is introduced, and then unique characteristics of plasma fuel reforming such as non-equilibrium product distribution is discussed.

  14. A Selection of Recent Advances in C1 Chemistry.

    PubMed

    Mesters, Carl

    2016-06-01

    This review presents a selection of recent publications related to the chemistry and catalysis of C1 molecules, including methane, methanol, carbon monoxide, and carbon dioxide. These molecules play an important role in the current supply of energy and chemicals and will likely become even more relevant because of the need to decarbonize fuels (shift from coal to natural gas) in line with CO2 capture and use to mitigate global warming, as well as a gradual shift on the supply side from crude oil to natural gas. This review includes both recent industrial developments, such as the huge increase in methanol-to-olefins-capacity build in China and the demonstration of oxidative coupling of methane, and scientific developments in these chemistries facilitated by improved capabilities in, for example, analytical tools and computational modeling. PMID:27276549

  15. Quantification of the Raf-C1 interaction with solid-supported bilayers.

    PubMed

    Eing, Andreas; Janshoff, Andreas; Galla, Hans-Joachim; Block, Christoph; Steinem, Claudia

    2002-03-01

    By use of the quartz crystal microbalance technique, the interaction of the Raf-Ras binding domain (RafRBD) and the cysteine-rich domain Raf-C1 with lipids was quantified by using solid-supported bilayers immobilized on gold electrodes deposited on 5 MHz quartz plates. Solid-supported lipid bilayers were composed of an initial octanethiol monolayer chemisorbed on gold and a physisorbed phospholipid monolayer varying in its lipid composition as the outermost layer. The integrity of bilayer preparation was monitored by impedance spectroscopy. For binding experiments, a protein construct comprising the RafRBD and Raf-C1 linked to the maltose binding protein and a His tag, termed MBP-Raf-C1, was used. Dissociation constants and rate constants of the association and dissociation were obtained for various 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC)/1,2-dimyristoyl-sn-glycero-3-phosphoserine (DMPS) lipid mixtures. Independently of the phosphatidylserine (PS) content, the dissociation constants were in the order of 5x10(-7) M, while the on-rate constants were in the range of 2x10(3) (M s)(-1) and the off-rate constants in the range of 1x10(-3) s(-1). The maximum frequency shift increased significantly with increasing amounts of DMPS; this indicates that this negatively charged lipid is the primary binding site for MBP-Raf-C1. Exchange of DMPS for 1,2-dimyristoyl-sn-glycero-3-phosphoglycerol (DMPG) did not alter the thermodynamics and kinetics of protein binding, which implies that the protein interaction is mainly electrostatically driven. Scanning force microscopy (SFM) was employed to render protein adsorption visible and to confirm the assumption of a protein monolayer on the lipid layer. SFM images clearly revealed that the protein binds preferentially, but not solely, to negatively charged phosphatidylserine headgroups. We hypothesize that PS-enriched domains are initial binding sites with high affinity for Raf-C1, but that lateral interactions may account for

  16. C(1) metabolism and CVD outcomes in older adults.

    PubMed

    McNulty, Helene; Strain, J J; Pentieva, Kristina; Ward, Mary

    2012-05-01

    CVD is the most common cause of death in people over 65 years. This review considers the latest evidence for a potential protective effect of C(1) donors (folate and the metabolically related B-vitamins) in CVD. Such an effect may or may not be mediated via the role of these nutrients in maintaining plasma homocysteine concentrations within a desirable range. Despite predictions from epidemiological studies that lowering plasma homocysteine would reduce cardiovascular risk, several secondary prevention trials in at-risk patients published since 2004 have failed to demonstrate a benefit of homocysteine-lowering therapy with B-vitamins on CVD events generally. All these trials were performed in CVD patients with advanced disease; thus current evidence suggests that intervention with high-dose folic acid is of no benefit in preventing another event, at least in the case of heart disease. The evidence at this time, however, is stronger for stroke, with meta-analyses of randomised trials showing that folic acid reduces the risk of stroke, particularly in people with no history of stroke. Genetic studies provide convincing evidence to support a causal relationship between sub-optimal B-vitamin status and CVD. People homozygous for the common C677T variant in the gene encoding the folate-metabolising enzyme, methylenetetrahydrofolate reductase (MTHFR), typically have a 14-21% higher risk of CVD. Apart from folate, riboflavin is required as a co-factor for MTHFR. New evidence shows that riboflavin intervention results in marked lowering of blood pressure, specifically in patients with the MTHFR 677TT genotype. This novel gene-nutrient interaction may provide insights as to the mechanism that links C(1) metabolism with CVD outcomes. PMID:22152927

  17. Improvement in the occupational health program in a Finnish construction company by means of systematic workplace investigation of job load and hazard analysis.

    PubMed

    Mattila, M

    1989-01-01

    The aim of the present study was to improve an occupational health program by means of systematic workplace investigations. The 8-month study was done at three building sites of one construction firm. The method for workplace investigations was a simple job hazard analysis of chemical hazards, physical hazards, physical work load, mental stress, and risk of injury, each factor being rated on a three-point scale. Information was gathered by observations, interviews, and a worker questionnaire. Occupational health and safety personnel and worker representatives dealt with problems cooperatively. Together they assessed occupational loads and hazards, whereafter the occupational health and safety personnel devised an occupational health care program and proposed preventive measures. The workplace investigation method proved to function well. It improved the occupational health care program, produced an overall analysis of occupational hazards, and dramatically increased the number and quality of proposed preventive measures. The new method was evaluated to be clearly superior to previous practices and was implemented at moderate cost. The study showed that attention to issues of environmental and occupational health can effectively prevent health impairment even in difficult setting such as construction work. More research is needed for effective utilization of information accumulated by systematic workplace investigations. PMID:2929609

  18. Improvement in the occupational health program in a Finnish construction company by means of systematic workplace investigation of job load and hazard analysis.

    PubMed

    Mattila, M

    1989-01-01

    The aim of the present study was to improve an occupational health program by means of systematic workplace investigations. The 8-month study was done at three building sites of one construction firm. The method for workplace investigations was a simple job hazard analysis of chemical hazards, physical hazards, physical work load, mental stress, and risk of injury, each factor being rated on a three-point scale. Information was gathered by observations, interviews, and a worker questionnaire. Occupational health and safety personnel and worker representatives dealt with problems cooperatively. Together they assessed occupational loads and hazards, whereafter the occupational health and safety personnel devised an occupational health care program and proposed preventive measures. The workplace investigation method proved to function well. It improved the occupational health care program, produced an overall analysis of occupational hazards, and dramatically increased the number and quality of proposed preventive measures. The new method was evaluated to be clearly superior to previous practices and was implemented at moderate cost. The study showed that attention to issues of environmental and occupational health can effectively prevent health impairment even in difficult setting such as construction work. More research is needed for effective utilization of information accumulated by systematic workplace investigations.

  19. Translating evidence based violence and drug use prevention to obesity prevention: development and construction of the Pathways program

    PubMed Central

    Sakuma, Kari-Lyn K.; Riggs, Nathaniel R.; Pentz, Mary Ann

    2012-01-01

    Effective school-based obesity prevention programs are needed to prevent and reduce the growing obesity risk among youth. Utilizing the evidence-rich areas of violence and substance use prevention, translation science may provide an efficient means for developing curricula across multiple health behaviors. This paper introduces Pathways to Health, a school-based obesity prevention program that was developed by translating from evidence-based violence and drug use prevention programs, Promoting Alternative THinking Strategies and the Midwestern Prevention Project STAR (STAR). We illustrate how a hypothesized underlying behavior change mechanism in two domains of risk behavior, violence and substance use, can be applied to obesity prevention. A 4-step translational process is provided and may be relevant for use in developing other curricula to address multiple health risk behaviors. Practical application and decision points are also provided. PMID:21987475

  20. Simplifying the construction of domain-specific automatic programming systems: The NASA automated software development workstation project

    NASA Technical Reports Server (NTRS)

    Allen, Bradley P.; Holtzman, Peter L.

    1988-01-01

    An overview is presented of the Automated Software Development Workstation Project, an effort to explore knowledge-based approaches to increasing software productivity. The project focuses on applying the concept of domain specific automatic programming systems (D-SAPSs) to application domains at NASA's Johnson Space Flight Center. A version of a D-SAPS developed in Phase 1 of the project for the domain of space station momentum management is described. How problems encountered during its implementation led researchers to concentrate on simplifying the process of building and extending such systems is discussed. Researchers propose to do this by attacking three observed bottlenecks in the D-SAPS development process through the increased automation of the acquisition of programming knowledge and the use of an object oriented development methodology at all stages of the program design. How these ideas are being implemented in the Bauhaus, a prototype workstation for D-SAPS development is discussed.

  1. Simplifying the construction of domain-specific automatic programming systems: The NASA automated software development workstation project

    NASA Technical Reports Server (NTRS)

    Allen, Bradley P.; Holtzman, Peter L.

    1987-01-01

    An overview is presented of the Automated Software Development Workstation Project, an effort to explore knowledge-based approaches to increasing software productivity. The project focuses on applying the concept of domain specific automatic programming systems (D-SAPSs) to application domains at NASA's Johnson Space Center. A version of a D-SAPS developed in Phase 1 of the project for the domain of space station momentum management is described. How problems encountered during its implementation led researchers to concentrate on simplifying the process of building and extending such systems is discussed. Researchers propose to do this by attacking three observed bottlenecks in the D-SAPS development process through the increased automation of the acquisition of programming knowledge and the use of an object oriented development methodology at all stages of the program design. How these ideas are being implemented in the Bauhaus, a prototype workstation for D-SAPS development is discussed.

  2. C1 Chemistry for the Production of Ultra-Clean Liquid Transportation Fuels and Hydrogen

    SciTech Connect

    Gerald P. Huffman

    2005-03-31

    Faculty and students from five universities--the University of Kentucky, University of Pittsburgh, University of Utah, West Virginia University, and Auburn University--are collaborating in a research program to develop C1 chemistry processes to produce ultra-clean liquid transportation fuels and hydrogen, the zero-emissions transportation fuel of the future. The feedstocks contain one carbon atom per molecular unit. They include synthesis gas (syngas), a mixture of carbon monoxide and hydrogen produced by coal gasification or reforming of natural gas, methane, methanol, carbon dioxide, and carbon monoxide. An important objective is to develop C1 technology for the production of liquid transportation fuel and hydrogen from domestically plentiful resources such as coal, coalbed methane, and natural gas. An Industrial Advisory Board with representatives from Chevron-Texaco, Eastman Chemical, Conoco-Phillips, the Air Force Research Laboratory, the U.S. Army National Automotive Center (Tank & Automotive Command--TACOM), and Tier Associates provides guidance on the practicality of the research. The current report presents results obtained in this research program during the six months of the subject contract from October 1, 2002 through March 31, 2003. The results are presented in thirteen detailed reports on research projects headed by various faculty members at each of the five CFFS Universities. Additionally, an Executive Summary has been prepared that summarizes the principal results of all of these projects during the six-month reporting period.

  3. C1 CHEMISTRY FOR THE PRODUCTION OF ULTRA-CLEAN LIQUID TRANSPORTATION FUELS AND HYDROGEN

    SciTech Connect

    Gerald P. Huffman

    2004-03-31

    Faculty and students from five universities--the University of Kentucky, University of Pittsburgh, University of Utah, West Virginia University, and Auburn University--are collaborating in a research program to develop C1 chemistry processes to produce ultra-clean liquid transportation fuels and hydrogen, the zero-emissions transportation fuel of the future. The feedstocks contain one carbon atom per molecular unit. They include synthesis gas (syngas), a mixture of carbon monoxide and hydrogen produced by coal gasification or reforming of natural gas, methane, methanol, carbon dioxide, and carbon monoxide. An important objective is to develop C1 technology for the production of liquid transportation fuel and hydrogen from domestically plentiful resources such as coal, coalbed methane, and natural gas. An Industrial Advisory Board with representatives from Chevron-Texaco, Eastman Chemical, Conoco-Phillips, the Air Force Research Laboratory, the U.S. Army National Automotive Center (Tank & Automotive Command--TACOM), and Tier Associates provides guidance on the practicality of the research. The current report presents results obtained in this research program during the six months of the subject contract from October 1, 2002 through March 31, 2003. The results are presented in thirteen detailed reports on research projects headed by various faculty members at each of the five CFFS Universities. Additionally, an Executive Summary has been prepared that summarizes the principal results of all of these projects during the six-month reporting period.

  4. C1 Chemistry for the Production of Ultra-Clean Liquid Transportation Fuels and Hydrogen

    SciTech Connect

    Gerald P. Huffman

    2003-03-31

    Faculty and students from five universities--the University of Kentucky, University of Pittsburgh, University of Utah, West Virginia University, and Auburn University--are collaborating in a research program to develop C1 chemistry processes to produce ultra-clean liquid transportation fuels and hydrogen, the zero-emissions transportation fuel of the future. The feedstocks contain one carbon atom per molecular unit. They include synthesis gas (syngas), a mixture of carbon monoxide and hydrogen produced by coal gasification or reforming of natural gas, methane, methanol, carbon dioxide, and carbon monoxide. An important objective is to develop C1 technology for the production of transportation fuel from domestically plentiful resources such as coal, coalbed methane, and natural gas. An Industrial Advisory Board with representatives from Chevron-Texaco, Eastman Chemical, Conoco-Phillips, Energy International, the Department of Defense, and Tier Associates provides guidance on the practicality of the research. The current report presents results obtained in this research program during the first six months of the subject contract (DE-FC26-02NT-4159), from October 1, 2002 through March 31, 2003.

  5. Measuring the Effectiveness of Transfer of Learning Constructs and Intent to Transfer in a Simulation-Based Leadership Training Program

    ERIC Educational Resources Information Center

    Hix, Joanne W.

    2013-01-01

    The purpose of business training programs is to improve performance, which improved performance changes leadership behaviors based on the knowledge, skills, and abilities (KSAs) learned in training. One of the most common criticisms of leadership training is the tendency to focus on teaching theory but not on applying theory into practice, that…

  6. Jackson District Library, Final Performance Report for Library Services and Construction Act (LSCA) Title VI, Library Literacy Program.

    ERIC Educational Resources Information Center

    Rosynek, Joy

    This final performance report provides project outcome information and data to the U.S. Department of Education for the federally-funded Library Literacy Program. The Jackson District Library (Michigan) conducted a project that involved recruitment, retention, public awareness, training, basic literacy, collection development, tutoring, employment…

  7. Hopkinsville-Christian County Library, Final Performance Report for Library Services and Construction Act (LSCA) Title VI, Library Literacy Program.

    ERIC Educational Resources Information Center

    Nevels, Vada Germaine

    The Hopkinsville-Christian County Library (Kentucky) conducted a project that involved recruitment, public awareness, basic literacy, collection development, tutoring, computer-assisted, other technology, and intergenerational/family programs. The project served a community of 50,000-100,000 people, and targeted the learning disabled,…

  8. Mesa County Public Library District, Final Performance Report for Library Services and Construction Act (LSCA) Title VI, Library Literacy Program.

    ERIC Educational Resources Information Center

    LaDuke, Caryl

    The Adult Reading Program, a project of the Mesa County Public Library District (Grand Junction, Colorado), involved recruitment, retention, coalition building, public awareness, training, rural oriented, basic literacy, collection development, tutoring, employment oriented, intergenerational/family, and English as a Second Language (ESL)…

  9. Wayne Township Public Library, Final Performance Report for Library Services and Construction Act (LSCA) Title VI, Library Literacy Program.

    ERIC Educational Resources Information Center

    Smyth, Carol B.; Grannell, Dorothy S.; Moore, Miriam

    The Literacy Resource Center project, a program of the Wayne Township Public Library also known as the Morrisson-Reeves Library (Richmond, Indiana), involved recruitment, retention, coalition building, public awareness, training, basic literacy, collection development, tutoring, computer-assisted, other technology, employment oriented,…

  10. Lee Library Association, Final Performance Report for Library Services and Construction Act (LSCA) Title VI, Library Literacy Program.

    ERIC Educational Resources Information Center

    Dalheim, Zoe; Mauke, Martha

    The Lee Library Association conducted a project that involved recruitment, public awareness, training, rural oriented, basic literacy, collection development, tutoring, computer assisted services, and English as a Second Language (ESL) programs. The project served a community of 25,000-50,000 people, and targeted the homeless, learning disabled,…

  11. Evangeline Parish Library, Final Performance Report for Library Services and Construction Act (LSCA) Title VI, Library Literacy Program, FY 1992.

    ERIC Educational Resources Information Center

    Fontenot, Jerry

    This final performance report provides project outcome information and data to the U.S. Department of Education for the federally-funded Library Literacy Program. The Evangeline Parish Library (Louisiana) conducted a project that involved recruitment, retention, public awareness, training, rural oriented, basic literacy, collection development,…

  12. Irvington Public Library, Final Performance Report for Library Services and Construction Act (LSCA) Title VI, Library Literacy Program.

    ERIC Educational Resources Information Center

    Shader, Holly

    The Irvington Public Library (Irvington, New Jersey) conducted a project that involved recruitment, retention, public awareness, training, basic literacy, collection development, tutoring, and intergenerational/family programs. The project served a community of 25,000-50,000 people, and targeted the learned disabled and intergenerational/families.…

  13. Marin County Free Library, Final Performance Report for Library Services and Construction Act (LSCA) Title VI, Library Literacy Program.

    ERIC Educational Resources Information Center

    Mooney, Sharon Lopez

    The West Marin Literacy Project, a project of the Marin County Free Library (San Rafael, California), involved recruitment, retention, coalition building, public awareness, training, rural oriented, tutoring, computer- assisted, intergenerational/family, and English as a Second Language (ESL) programs. The project served a community of under…

  14. Constructing Knowledge with an Agent-Based Instructional Program: A Comparison of Cooperative and Individual Meaning Making

    ERIC Educational Resources Information Center

    Moreno, Roxana

    2009-01-01

    Participants in the present study were 87 college students who learned about botany using an agent-based instructional program with three different learning approaches: individual, jigsaw, or cooperative learning. Results showed no differences among learning approaches on retention. Students in jigsaw groups reported higher cognitive load during…

  15. Pikes Peak Library District, Final Performance Report for Library Services and Construction Act (LSCA) Title VI, Library Literacy Program.

    ERIC Educational Resources Information Center

    Hammond, Susan; Maio, Jean

    This final performance report for the Pikes Peak Library District literacy project begins with a section that provides quantitative data. The next section compares actual accomplishments to the project goal and related objectives for 1993: to reduce obstacles to success of students enrolled in the Right To Read program through student…

  16. Orange Public Library, Final Performance Report for Library Services and Construction Act (LSCA) Title VI, Library Literacy Program.

    ERIC Educational Resources Information Center

    Miller, Rosanne

    The Orange Public Library (California) conducted a project that involved recruitment, retention, coalition building, public awareness, training, basic literacy, collection development, tutoring, intergenerational/family, and English as a Second Language (ESL) programs. The project served a community of 100,000-200,000 and targeted…

  17. Columbia County Public Library, Final Performance Report for Library Services and Construction Act (LSCA) Title VI, Library Literacy Program.

    ERIC Educational Resources Information Center

    Cole, Lucy; Fraser, Ruth

    The Columbia County Public Library (Lake City, Florida) conducted a project that involved recruitment, retention, public awareness, training, basic literacy, collection development, tutoring, computer- assisted, other technology, intergenerational/family, and English as a Second Language (ESL) programs. The project served a community of…

  18. Elizabeth Jones Library, Final Performance Report for Library Services and Construction Act (LSCA) Title VI, Library Literacy Program.

    ERIC Educational Resources Information Center

    Smith, Valinda C.

    This final performance report for the Elizabeth Jones Library literacy project begins with a section that provides quantitative data. The next section compares actual accomplishments to the following project goals and related objectives for fiscal year 1992: (1) to provide a versatile workplace literacy program useful to any business in the…

  19. Jefferson-Madison Regional Library, Final Performance Report for Library Services and Construction Act (LSCA) Title VI, Library Literacy Program.

    ERIC Educational Resources Information Center

    Morris, Karen L.; Porter, Connie

    The Jefferson-Madison Regional Library (Charlottesville, Virginia) conducted a project that involved training, basic literacy, collection development, tutoring, and computer assisted programs. The project served a population of 100,000-200,000, and targeted the homeless, workforce/workplace learning, and adult non- and new-readers. Tutoring was…

  20. Stratford Library Association, Final Performance Report for Library Services and Construction Act (LSCA) Title VI, Library Literacy Program.

    ERIC Educational Resources Information Center

    Moren, Barbara

    This final performance report provides project outcome information and data to the U.S. Department of Education for the federally-funded Library Literacy Program. The Stratford Library Association (Connecticut) conducted a project that involved recruitment, retention, training, basic literacy, collection development, tutoring,…

  1. Lebanon Community Library, Final Performance Report for Library Services and Construction Act (LSCA) Title VI, Library Literacy Program.

    ERIC Educational Resources Information Center

    Kline, Patricia A.; Long, Sandy

    The Lebanon County Family Literacy Program, a project of the Lebanon Community Library (Pennsylvania), targeted intergenerational/families and focused on coalition building, public awareness, training, and collection development. The Lebanon Community Library serves as: a centralized location for storing and disseminating materials and handouts on…

  2. Translating Evidence Based Violence and Drug Use Prevention to Obesity Prevention: Development and Construction of the Pathways Program

    ERIC Educational Resources Information Center

    Sakuma, Kari-Lyn K.; Riggs, Nathaniel R.; Pentz, Mary Ann

    2012-01-01

    Effective school-based obesity prevention programs are needed to prevent and reduce the growing obesity risk among youth. Utilizing the evidence-rich areas of violence and substance use prevention, translation science may provide an efficient means for developing curricula across multiple health behaviors. This paper introduces Pathways to Health,…

  3. Darboux transformations of lower degree for two-dimensional C(1)l and D(2)l+1 Toda equations

    NASA Astrophysics Data System (ADS)

    Zhou, Zi-Xiang

    2008-08-01

    For the two-dimensional Toda equations corresponding to the Kac-Moody algebras C(1)l and D(2)l+1, the Darboux transformations with a special choice of spectral parameters are constructed so that the degree of these Darboux transformations is half of that for usual Darboux transformations and the derived solutions become simpler. These Darboux transformations for a Lax pair are constructed from real solutions of itself or a slightly different Lax pair corresponding to the same Toda equation, depending on the parity of l. Exact solutions of these Toda equations are presented by simplifying the results derived from the Darboux transformations.

  4. Near-ultraviolet spectroscopy of Comet Austin (1989c1)

    NASA Technical Reports Server (NTRS)

    Valk, Jacobus H.; O'Dell, C. R.; Cochran, Anita L.; Cochran, William D.; Opal, Chet B. S.; Barker, Edwin S.

    1992-01-01

    Comet Austin (1989c1) was observed post-perihelion at a heliocentric distance near 1.25 AU. The wavelength range was from the atmospheric cutoff at 3000 to 4000 A. The coma spectra were calibrated into flux units and the contaminating sky spectrum and solar scattered light continuum were subtracted, leaving an ultraviolet spectrum of about 1.5-A resolution and excellent signal-to-noise ratio. The spectrum is dominated by emissions from OH, NH, CH, C3, and CN, some of the weaker emissions of which are seen here for the first time. More bands of CO(2+) were found than in any previous investigation and several intensity anomalies were noted; H2CO, OH(+), NCN, N(2+), and CN(+) may be present. Several emission features well above the noise level remain unidentified. The relative intensities of the OH and CN bands agree with the predictions of resonance fluorescence when one considers the potential effects of contamination by other molecules. The effects of the ozone absorption spectrum are not fully removed by the data-reduction process, although this does not affect these results.

  5. Structure of human Niemann-Pick C1 protein.

    PubMed

    Li, Xiaochun; Wang, Jiawei; Coutavas, Elias; Shi, Hang; Hao, Qi; Blobel, Günter

    2016-07-19

    Niemann-Pick C1 protein (NPC1) is a late-endosomal membrane protein involved in trafficking of LDL-derived cholesterol, Niemann-Pick disease type C, and Ebola virus infection. NPC1 contains 13 transmembrane segments (TMs), five of which are thought to represent a "sterol-sensing domain" (SSD). Although present also in other key regulatory proteins of cholesterol biosynthesis, uptake, and signaling, the structure and mechanism of action of the SSD are unknown. Here we report a crystal structure of a large fragment of human NPC1 at 3.6 Å resolution, which reveals internal twofold pseudosymmetry along TM 2-13 and two structurally homologous domains that protrude 60 Å into the endosomal lumen. Strikingly, NPC1's SSD forms a cavity that is accessible from both the luminal bilayer leaflet and the endosomal lumen; computational modeling suggests that this cavity is large enough to accommodate one cholesterol molecule. We propose a model for NPC1 function in cholesterol sensing and transport. PMID:27307437

  6. Structure of human Niemann–Pick C1 protein

    PubMed Central

    Li, Xiaochun; Wang, Jiawei; Coutavas, Elias; Shi, Hang; Hao, Qi; Blobel, Günter

    2016-01-01

    Niemann–Pick C1 protein (NPC1) is a late-endosomal membrane protein involved in trafficking of LDL-derived cholesterol, Niemann–Pick disease type C, and Ebola virus infection. NPC1 contains 13 transmembrane segments (TMs), five of which are thought to represent a “sterol-sensing domain” (SSD). Although present also in other key regulatory proteins of cholesterol biosynthesis, uptake, and signaling, the structure and mechanism of action of the SSD are unknown. Here we report a crystal structure of a large fragment of human NPC1 at 3.6 Å resolution, which reveals internal twofold pseudosymmetry along TM 2–13 and two structurally homologous domains that protrude 60 Å into the endosomal lumen. Strikingly, NPC1's SSD forms a cavity that is accessible from both the luminal bilayer leaflet and the endosomal lumen; computational modeling suggests that this cavity is large enough to accommodate one cholesterol molecule. We propose a model for NPC1 function in cholesterol sensing and transport. PMID:27307437

  7. Preliminary design data package, appendices C1 and C2

    NASA Technical Reports Server (NTRS)

    1980-01-01

    The HYBRID2 program which computes the fuel and energy consumption of a hybrid vehicle with a bi-modal control strategy over specified component driving cycles is described. Fuel and energy consumption are computed separately for the two modes of operation. The program also computes yearly average fuel and energy consumption using a composite driving cycle which varies as a function of daily travel. The modelling techniques are described, and subroutines and their functions are given. The composition of modern automobiles is discussed along with the energy required to manufacture an American automobile. The energy required to scrap and recycle automobiles is also discussed.

  8. The conversion of C'IS to C'1 esterase by plasmin and trypsin.

    PubMed

    Ratnoff, O D; Naff, G B

    1967-02-01

    The formation of C'1 esterase from C'1, the first component of complement, may be brought about by the action of plasmin or trypsin upon C'1s, a subcomponent of C'1. These enzymes also decrease the esterolytic activity of C'1 esterase. The formation of C'1 esterase was demonstrated by measuring the appearance of an agent or agents with esterolytic properties and the capacity to inactivate C'2 and C'4, attributes of C'1 esterase. The activity of the agent which evolved was blocked by serum inhibitor of C'1 esterase. The implications of these observations, that the formation of C'1 esterase during complement fixation is mediated by proteolytic processes, are under study. The possible inhibition of C'1q by soybean trypsin inhibitor is in agreement with this hypothesis.

  9. Haploinsufficiency for NR3C1, the gene encoding the glucocorticoid receptor, in blastic plasmacytoid dendritic cell neoplasms.

    PubMed

    Emadali, Anouk; Hoghoughi, Neda; Duley, Samuel; Hajmirza, Azadeh; Verhoeyen, Els; Cosset, Francois-Loic; Bertrand, Philippe; Roumier, Christophe; Roggy, Anne; Suchaud-Martin, Céline; Chauvet, Martine; Bertrand, Sarah; Hamaidia, Sieme; Rousseaux, Sophie; Josserand, Véronique; Charles, Julie; Templier, Isabelle; Maeda, Takahiro; Bruder-Costa, Juliana; Chaperot, Laurence; Plumas, Joel; Jacob, Marie-Christine; Bonnefoix, Thierry; Park, Sophie; Gressin, Remy; Tensen, Cornelis P; Mecucci, Cristina; Macintyre, Elizabeth; Leroux, Dominique; Brambilla, Elisabeth; Nguyen-Khac, Florence; Luquet, Isabelle; Penther, Dominique; Bastard, Christian; Jardin, Fabrice; Lefebvre, Christine; Garnache, Francine; Callanan, Mary B

    2016-06-16

    Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare and highly aggressive leukemia for which knowledge on disease mechanisms and effective therapies are currently lacking. Only a handful of recurring genetic mutations have been identified and none is specific to BPDCN. In this study, through molecular cloning in an index case that presented a balanced t(3;5)(q21;q31) and molecular cytogenetic analyses in a further 46 cases, we identify monoallelic deletion of NR3C1 (5q31), encoding the glucocorticoid receptor (GCR), in 13 of 47 (28%) BPDCN patients. Targeted deep sequencing in 36 BPDCN cases, including 10 with NR3C1 deletion, did not reveal NR3C1 point mutations or indels. Haploinsufficiency for NR3C1 defined a subset of BPDCN with lowered GCR expression and extremely poor overall survival (P = .0006). Consistent with a role for GCR in tumor suppression, functional analyses coupled with gene expression profiling identified corticoresistance and loss-of-EZH2 function as major downstream consequences of NR3C1 deletion in BPDCN. Subsequently, more detailed analyses of the t(3;5)(q21;q31) revealed fusion of NR3C1 to a long noncoding RNA (lncRNA) gene (lincRNA-3q) that encodes a novel, nuclear, noncoding RNA involved in the regulation of leukemia stem cell programs and G1/S transition, via E2F. Overexpression of lincRNA-3q was a consistent feature of malignant cells and could be abrogated by bromodomain and extraterminal domain (BET) protein inhibition. Taken together, this work points to NR3C1 as a haploinsufficient tumor suppressor in a subset of BPDCN and identifies BET inhibition, acting at least partially via lncRNA blockade, as a novel treatment option in BPDCN.

  10. Haploinsufficiency for NR3C1, the gene encoding the glucocorticoid receptor, in blastic plasmacytoid dendritic cell neoplasms

    PubMed Central

    Emadali, Anouk; Hoghoughi, Neda; Duley, Samuel; Hajmirza, Azadeh; Verhoeyen, Els; Cosset, Francois-Loic; Bertrand, Philippe; Roumier, Christophe; Roggy, Anne; Suchaud-Martin, Céline; Chauvet, Martine; Bertrand, Sarah; Hamaidia, Sieme; Rousseaux, Sophie; Josserand, Véronique; Charles, Julie; Templier, Isabelle; Maeda, Takahiro; Bruder-Costa, Juliana; Chaperot, Laurence; Plumas, Joel; Jacob, Marie-Christine; Bonnefoix, Thierry; Park, Sophie; Gressin, Remy; Tensen, Cornelis P.; Mecucci, Cristina; Macintyre, Elizabeth; Leroux, Dominique; Brambilla, Elisabeth; Nguyen-Khac, Florence; Luquet, Isabelle; Penther, Dominique; Bastard, Christian; Jardin, Fabrice; Lefebvre, Christine; Garnache, Francine

    2016-01-01

    Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare and highly aggressive leukemia for which knowledge on disease mechanisms and effective therapies are currently lacking. Only a handful of recurring genetic mutations have been identified and none is specific to BPDCN. In this study, through molecular cloning in an index case that presented a balanced t(3;5)(q21;q31) and molecular cytogenetic analyses in a further 46 cases, we identify monoallelic deletion of NR3C1 (5q31), encoding the glucocorticoid receptor (GCR), in 13 of 47 (28%) BPDCN patients. Targeted deep sequencing in 36 BPDCN cases, including 10 with NR3C1 deletion, did not reveal NR3C1 point mutations or indels. Haploinsufficiency for NR3C1 defined a subset of BPDCN with lowered GCR expression and extremely poor overall survival (P = .0006). Consistent with a role for GCR in tumor suppression, functional analyses coupled with gene expression profiling identified corticoresistance and loss-of-EZH2 function as major downstream consequences of NR3C1 deletion in BPDCN. Subsequently, more detailed analyses of the t(3;5)(q21;q31) revealed fusion of NR3C1 to a long noncoding RNA (lncRNA) gene (lincRNA-3q) that encodes a novel, nuclear, noncoding RNA involved in the regulation of leukemia stem cell programs and G1/S transition, via E2F. Overexpression of lincRNA-3q was a consistent feature of malignant cells and could be abrogated by bromodomain and extraterminal domain (BET) protein inhibition. Taken together, this work points to NR3C1 as a haploinsufficient tumor suppressor in a subset of BPDCN and identifies BET inhibition, acting at least partially via lncRNA blockade, as a novel treatment option in BPDCN. PMID:27060168

  11. Studies on the haemolytic activity of circulating C1q-C3/C4 complexes.

    PubMed

    Wouters, Diana; Brouwer, Mieke C; Daha, Mohamed R; Hack, C Erik

    2008-04-01

    During classical complement pathway activation, the internal thio-ester of both C3 and C4 becomes exposed which enables C3 and C4 to bind covalently to nearby molecules. Recently, we described that C3 and C4 bind to C1q, the recognition molecule of the classical pathway, upon activation of this pathway. Covalently linked complexes between C1q and activated C4 (C1q-C4 complexes) are specific markers for classical complement pathway activation. In the present study we further investigated the molecular characteristics of complexes between C1q and activated C3 or C4 that occur in vivo. In human serum only complexes of C1q with C3d or C4d fragments were detected but not those with the larger C3b/bi or C4b/bi fragments. We identified that C1q-C4 complexes circulate as part of the intact C1 complex instead of as free C1q. Finally, we investigated whether deposited C3d or C4d affect C1 haemolytic activity. We observed that both C1q-C3 and C1q-C4 complexes are significantly (P<0.05) less active in a C1q-haemolytic assay than non-complexed C1q. Thus, the dominant types of C1q complexes that circulate in vivo are C1q-C3d and C1q-C4d complexes. These complexes are still able to interact with C1r and C1s to form a C1 complex, but seem to have a reduced activity as compared to C1q not carrying C3- or C4-fragments.

  12. 15 CFR Schedule I to Part 700 - Approved Programs and Delegate Agencies

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Trade (Continued) BUREAU OF INDUSTRY AND SECURITY, DEPARTMENT OF COMMERCE NATIONAL SECURITY INDUSTRIAL... (Government owned) Do. C1 Food resources (combat rations) Do. C2 Department of Defense construction Do. C3... Designated Programs Do. K1 Federal supply items General Services Administration. Homeland security...

  13. Transcriptional regulation of aldo-keto reductase 1C1 in HT29 human colon cancer cells resistant to methotrexate: role in the cell cycle and apoptosis.

    PubMed

    Selga, Elisabet; Noé, Véronique; Ciudad, Carlos J

    2008-01-15

    While studying differentially expressed genes between sensitive and 10(-5)M Methotrexate (MTX) resistant HT29 human colon cancer cells, we identified some members of the aldo-keto reductase (AKR) superfamily. The study was followed with the member AKR1C1 (EC 1.1.1.213), validating its increase in mRNA and protein levels in MTX resistant cells. The genomic content for AKR1C1 remained unchanged between sensitive and resistant cells, thereby excluding a mechanism of AKR1C1 gene amplification. Thus, we cloned the AKR1C1 human promoter and performed luciferase experiments that revealed a transcriptional regulation of the gene in the resistant cells. Computational studies showed a putative binding site for the transcription factor Sp1. The co-transfection of Sp1 or Sp3 with different constructs of AKR1C1 promoter deletions, including and excluding the proximal GC-box, demonstrated a key role for these factors in regulating AKR1C1 transcriptional activity. Gel-shift assays revealed an increase in Sp1 and Sp3 binding in resistant compared to sensitive cells, without differences in Sp1 protein levels. Dephosphorylation of the extracts coincided with a decrease in Sp1 binding, which is consistent with a process of regulation of Sp1 by phosphorylation. We also investigated the possible relationship between AKR1C1 expression and MTX action. Overexpression of AKR1C1 counteracted the S-phase accumulation of cells and apoptosis caused by MTX treatment. This suggests a role of AKR1C1 in cell proliferation. Finally, overexpression of AKR1C1 in MTX sensitive HT29 cells conferred resistance to the chemotherapeutic agent and silencing of AKR1C1 by means of iRNA technology sensitized the cells to MTX.

  14. Molecular analysis of protein domain function encoded by the myb-homologous maize genes C1, Zm 1 and Zm 38.

    PubMed

    Franken, P; Schrell, S; Peterson, P A; Saedler, H; Wienand, U

    1994-07-01

    Two maize genes, Zm 1 and Zm 38, related to the regulatory anthocyanin gene C1 were analyzed molecularly and used for fusion constructs in transient domain swapping experiments with the C1 wild-type gene. It was shown that both genes (Zm 1 and Zm 38) influence the expression of the A1 locus, a target gene for C1. Zm 1 activates the A1 promoter, however it does not turn on the whole anthocyanin pathway. The Zm 38 gene product shows functions similar to C1-I, a dominant inhibitor of the C1 wild-type gene. Concerning the trans-inhibition by C1-I two effects seem to be involved, competition for binding and formation of heterodimers. Further analysis of C1 function was carried out by a fine structure analysis of C1 mutants induced by the insertion and excision of transposable elements. These experiments indicate that for the activating domain of the protein, the formation of an alpha helix seems to be more important than a high negative charge.

  15. Recombinant C1 inhibitor P5/P3 variants display resistance to catalytic inactivation by stimulated neutrophils.

    PubMed

    Eldering, E; Huijbregts, C C; Nuijens, J H; Verhoeven, A J; Hack, C E

    1993-03-01

    Proteolytic inactivation of serine protease inhibitors (serpins) by neutrophil elastase (HNE) is presumed to contribute to the deregulation of plasma cascade systems in septic shock. Here, we report a supplementary approach to construct serpins, in our case C1 inhibitor, that are resistant to catalytic inactivation by HNE. Instead of shifting the specificity of alpha 1-antitrypsin towards the proteases of the contact activation and complement systems, we attempted to obtain a C1 inhibitor species which resists proteolytic inactivation by HNE. 12 recombinant C1 inhibitor variants were produced with mainly conservative substitutions at the cleavage sites for HNE, 440-Ile and/or 442-Val. Three variants significantly resisted proteolytic inactivation, both by purified HNE, as well as by activated neutrophils. The increase in functional half-life in the presence of FMLP-stimulated cells was found to be 18-fold for the 440-Leu/442-Ala variant. Inhibitory function of these variants was relatively unimpaired, as examined by the formation of stable complexes with C1s, beta-Factor XIIa, kallikrein, and plasmin, and as determined by kinetic analysis. The calculated association rate constants (k(on)) were reduced twofold at most for C1s, and appeared unaffected for beta-Factor XIIa. The effect on the k(on) with kallikrein was more pronounced, ranging from a significant ninefold reduction to an unmodified rate. The results show that the reactive centre loop of C1 inhibitor can be modified towards decreased sensitivity for nontarget proteases without loss of specificity for target proteases. We conclude that this approach extends the possibilities of applying recombinant serpin variants for therapeutic use in inflammatory diseases.

  16. Synthesis of C3/C1-Substituted Tetrahydroisoquinolines.

    PubMed

    Mihoubi, Mohamed; Micale, Nicola; Scala, Angela; Jarraya, Raoudha Mezghani; Bouaziz, Amira; Schirmeister, Tanja; Risitano, Francesco; Piperno, Anna; Grassi, Giovanni

    2015-01-01

    A broad biological screening of the natural alkaloid N-methylisosalsoline (2) extracted from Hammada scoparia leaves against a panel of human and parasitic proteases revealed an interesting activity profile of 2 towards human 20S proteasome. This outcome suggests that the 1,2,3,4-tetrahydroisoquinoline skeleton may be exploited as a template for the development of novel anticancer agents. In this article, we report the synthesis and chemical characterization of a new series of isosalsoline-type alkaloids (10-11) with variations at N2 and C3 positions with respect to the natural Compound 2, obtained by a synthetic strategy that involves the Bischler-Napieralski cyclization. The substrate for the condensation to the tetrahydroisoquinoline system, i.e., a functionalized β-arylethyl amine, was obtained through an original double reduction of nitroalkene. The synthetic strategy can be directed to the construction of highly substituted and functionalized 1,2,3,4-tetrahydroisoquinolines. PMID:26287146

  17. Self-assembly of Carbon Vacancies in Sub-stoichiometric ZrC1−x

    PubMed Central

    Zhang, Yanhui; Liu, Bin; Wang, Jingyang

    2015-01-01

    Sub-stoichiometric interstitial compounds, including binary transition metal carbides (MC1−x), maintain structural stability even if they accommodate abundant anion vacancies. This unique character endows them with variable-composition, diverse-configuration and controllable-performance through composition and structure design. Herein, the evolution of carbon vacancy (VC) configuration in sub-stoichiometric ZrC1−x is investigated by combining the cluster expansion method and first-principles calculations. We report the interesting self-assembly of VCs and the fingerprint VC configuration (VC triplet constructed by 3rd nearest neighboring vacancies) in all the low energy structures of ZrC1−x. When VC concentration is higher than the critical value of 0.5 (x > 0.5), the 2nd nearest neighboring VC configurations with strongly repulsive interaction inevitably appear, and meanwhile, the system energy (or formation enthalpy) of ZrC1−x increases sharply which suggests the material may lose phase stability. The present results clarify why ZrC1−x bears a huge amount of VCs, tends towards VC ordering, and retains stability up to a stoichiometry of x = 0.5. PMID:26667083

  18. C1 CHEMISTRY FOR THE PRODUCTION OF ULTRA-CLEAN LIQUID TRANSPORTATION FUELS AND HYDROGEN

    SciTech Connect

    Gerald P. Huffman

    2004-09-30

    The Consortium for Fossil Fuel Science (CFFS) is a research consortium with participants from the University of Kentucky, University of Pittsburgh, West Virginia University, University of Utah, and Auburn University. The CFFS is conducting a research program to develop C1 chemistry technology for the production of clean transportation fuel from resources such as coal and natural gas, which are more plentiful domestically than petroleum. The processes under development will convert feedstocks containing one carbon atom per molecular unit into ultra clean liquid transportation fuels (gasoline, diesel, and jet fuel) and hydrogen, which many believe will be the transportation fuel of the future. Feedstocks include synthesis gas, a mixture of carbon monoxide and hydrogen produced by coal gasification, coalbed methane, light products produced by Fischer-Tropsch (FT) synthesis, methanol, and natural gas.

  19. Influence of ionic strength, EDTA concentration, endogenous C1q and polyanions on the 125I-C1q-binding test.

    PubMed

    Hack, C E; Huijbregts, C C; Paardekooper, J

    1984-08-01

    Several parameters of the 125I-C1q-binding test were investigated: ionic strength, pH, concentration of EDTA, influence of serum C1q and the possibility of interference by polyanions. Lowering the ionic strength of the borate buffer resulted in increased precipitation of 125I-C1q in normal human serum. This increase was dependent on the presence of serum proteins, probably immunoglobulins. When the concentration of the EDTA was decreased, increased precipitation of 125I-C1q in normal human serum was also observed. This was prevented by adding NaCl to the EDTA solution. However at very low concentrations of EDTA (too low to chelate all calcium ions in the serum), increased precipitation of 125I-C1q in normal human serum was observed even in the presence of added NaCl. Addition of purified C1q to sera from patients with very low C1q levels had varying effects on the results of the C1q-binding test: (a) it decreased the C1q-binding activity of some sera, probably by competition with 125I-C1q for binding sites on the immune complexes; (b) it increased the C1q-binding activity of other sera, probably by enhancing the precipitation of immune complexes as a consequence of the cross-linking effect of C1q; or (c) it had no influence, possibly due to the opposite effects of (a) and (b). The addition of dextran sulphate resulted in a dose-dependent increase in the 125I-C1q-binding activity of normal human serum. This effect was dependent on the interaction of dextran sulphate with either C1q or low-density lipoproteins and was prevented by addition of polybrene to the assay. However, addition of polybrene to sera with a high C1q-binding activity scarcely influenced binding activity.

  20. Construction of knowledge about teaching practice and educating students from diverse cultures in an online induction program

    NASA Astrophysics Data System (ADS)

    Bice, Lawrence Raymond

    2005-11-01

    Beginning teachers in both urban areas and geographically isolated rural areas often do not have access to a mentor teacher of the same content area or grade level in their school or district. This project is a study of learning in the on-line e-Mentoring for Student Success (eMSS) program, which provides induction for science and mathematics teachers in Montana and California. The study centered on a particular segment of eMSS called the Diversity Module. Two examinations were conducted: (1) Analysis of discourse by all participants in the Diversity Module, and (2) case study of five beginning teachers with diverse student populations. Analysis of learning by cases was conducted by examining discourse in the Diversity Module, private on-line discussions with their assigned mentors during a two-year period, and pre and post Diversity Module interviews and interviews of their mentors. Three frameworks were developed to aid understanding of findings: (1) discourse analysis, (2) competencies of multicultural teachers, and, (3) competencies of pedagogical and pedagogical content knowledge. Cases developed their knowledge of teaching along a continuum of needs over two years of participation in the eMSS program. Initial needs expressed by mentees were in areas such as classroom management and general methods of instruction. Cases increased their knowledge in virtually all aspects of pedagogical knowledge, changing their expressed needs to pedagogical content knowledge concerns such as adapting and differentiating instruction for particular content and individual students, and building their repertoire of instructional representations. Through on-line discussion, teachers developed or advanced awareness of student culture and learning characteristics, and adapted their practice to foster a climate of student respect. Findings provided little evidence of adapting instruction for diverse student learning. Teachers who had a strong awareness of their own and their students

  1. Significance of low molecular weight C1q in systemic lupus erythematosus.

    PubMed

    Hoekzema, R; Swaak, A J; Brouwer, M C; van Rooijen, A; Nieuwenhuys, E J; Hack, C E

    1990-09-01

    The significance of high serum concentrations of low molecular weight C1q (LMW-C1q) in patients with systemic lupus erythematosus (SLE) was studied. Concentrations of LMW-C1q were increased in SLE, but not in rheumatoid arthritis or acute poststreptococcal glomerulonephritis. Concentrations of LMW-C1q in SLE serum samples correlated with titres of anti-dsDNA and were inversely related to concentrations of normal C1q and C3. Serial studies in six patients, who had rising anti-dsDNA titres and who developed a major exacerbation requiring admission to hospital, showed that LMW-C1q increased in parallel with anti-dsDNA, reaching peak values of more than 2000% of normal just before or at the time of clinical relapse and decreasing during convalescence. Most marked increases in LMW-C1q were noted in the three patients in whom C1q concentrations remained normal, whereas increases were less in the three patients who had strongly depressed concentrations of normal C1q. A study of C1q biosynthesis by macrophages cultured from patients with SLE and high serum concentrations of LMW-C1q did not show impaired secretion of normal C1q in favour of LMW-C1q, but indicated that serum concentrations of LMW-C1q may reflect the synthetic rate of C1q in vivo. The results show that increased serum concentrations of LMW-C1q may be helpful in diagnosing SLE and suggest that serial determination of LMW-C1q in serum may have predictive value in monitoring patients with SLE.

  2. In silico structural characteristics and α-amylase inhibitory properties of Ric c 1 and Ric c 3, allergenic 2S albumins from Ricinus communis seeds.

    PubMed

    Do Nascimento, Viviane Veiga; Castro, Helena Carla; Abreu, Paula Alvarez; Oliveira, Antônia Elenir Amâncio; Fernandez, Jorge Hernandez; Araújo, Jucélia Da Silva; Machado, Olga Lima Tavares

    2011-05-11

    The major Ricinus communis allergens are the 2S albumins, Ric c 1 and Ric c 3. These proteins contain a trypsin/α-amylase inhibitor family domain, suggesting that they have a role in insect resistance. In this study, we verified that Ric c 1 and Ric c 3 inhibited the α-amylase activity of Callosobruchus maculatus, Zabrotes subfasciatus, and Tenebrio molitor (TMA) larvae as well as mammalian α-amylase. The toxicity of 2S albumin was determined through its incorporation in C. maculatus larvae as part of an artificial diet. Bioassays revealed that 2S albumin reduced larval growth by 20%. We also analyzed the tridimensional structures of Ric c 1 and Ric c 3 by (a) constructing a comparative model of Ric c 1 based on Ric c 3 NMR structure and (b) constructing the theoretical structure of the Ric c 1-TMA and Ric c 3-TMA complexes. Our biological and theoretical results revealed that Ric c 1 and Ric c 3 are a new class of α-amylase inhibitors. They could potentially be used to help design inhibitors that would be useful in diverse fields, ranging from diabetes treatment to crop protection.

  3. rhC1INH: a new drug for the treatment of attacks in hereditary angioedema caused by C1-inhibitor deficiency.

    PubMed

    Varga, Lilian; Farkas, Henriette

    2011-03-01

    Recombinant human C1 esterase inhibitor (rhC1INH) (Ruconest(®), Pharming) is a new drug developed for the relief of symptoms occurring in patients with angioedema due to C1-inhibitor deficiency. Pertinent results have already been published elsewhere; this article summarizes the progress made since then. Similar to the purified C1-inhibitor derived from human plasma, the therapeutic efficacy of rhC1INH results from its ability to block the actions of enzymes belonging to the overactivated bradykinin-forming pathway, at multiple locations. During clinical trials into the management of acute edema, a total of 190 subjects received recombinant C1-inhibitor by intravenous infusion on 714 occasions altogether. Dose-ranging efficacy studies established 50 U/kg as the recommended dose, and demonstrated the effectiveness of this agent in all localizations of hereditary angioedema attacks. Studies into the safety of rhC1INH based on 300 administrations to healthy subjects or hereditary angioedema patients followed-up for 90 days have not detected the formation of autoantibodies against rhC1INH or IgE antibodies directed against rabbit proteins, even after repeated administration on multiple occasions. These findings met favorable appraisal by the EMA, which granted European marketing authorization for rhC1INH. Pharming is expected to file a biological licence with the US FDA by the end of 2010 to obtain marketing approval in the USA. The launch of rhC1INH onto the pharmaceutical market may represent an important progress in the management of hereditary angioedema patients.

  4. rhC1INH: a new drug for the treatment of attacks in hereditary angioedema caused by C1-inhibitor deficiency.

    PubMed

    Varga, Lilian; Farkas, Henriette

    2011-03-01

    Recombinant human C1 esterase inhibitor (rhC1INH) (Ruconest(®), Pharming) is a new drug developed for the relief of symptoms occurring in patients with angioedema due to C1-inhibitor deficiency. Pertinent results have already been published elsewhere; this article summarizes the progress made since then. Similar to the purified C1-inhibitor derived from human plasma, the therapeutic efficacy of rhC1INH results from its ability to block the actions of enzymes belonging to the overactivated bradykinin-forming pathway, at multiple locations. During clinical trials into the management of acute edema, a total of 190 subjects received recombinant C1-inhibitor by intravenous infusion on 714 occasions altogether. Dose-ranging efficacy studies established 50 U/kg as the recommended dose, and demonstrated the effectiveness of this agent in all localizations of hereditary angioedema attacks. Studies into the safety of rhC1INH based on 300 administrations to healthy subjects or hereditary angioedema patients followed-up for 90 days have not detected the formation of autoantibodies against rhC1INH or IgE antibodies directed against rabbit proteins, even after repeated administration on multiple occasions. These findings met favorable appraisal by the EMA, which granted European marketing authorization for rhC1INH. Pharming is expected to file a biological licence with the US FDA by the end of 2010 to obtain marketing approval in the USA. The launch of rhC1INH onto the pharmaceutical market may represent an important progress in the management of hereditary angioedema patients. PMID:21426252

  5. Do the mutations of C1GALT1C1 gene play important roles in the genetic susceptibility to Chinese IgA nephropathy?

    PubMed Central

    Li, Gui-Sen; Nie, Guang-Jun; Zhang, Hong; LV, Ji-Cheng; Shen, Yan; Wang, Hai-Yan

    2009-01-01

    Background The deficiency of β1,3 galactose in hinge region of IgA1 molecule played a pivotal role in pathogenesis of IgA nephropathy (IgAN). Cosmc, encoded by C1GALT1C1 gene, was indispensable to β1,3 galactosylation of IgA1. We designed a serial study to investigate the relationship between the mutations of C1GALT1C1 gene and the genetic susceptibility to IgAN. Methods Nine hundred and thirty-eight subjects, including 661 patients with IgAN and 277 healthy controls were enrolled in the study. Firstly, single nucleotide polymorphisms (SNPs) in the promoter region of C1GALT1C1 gene were screened. Then the c.-347-190G>A was analyzed by PCR-restriction fragment length polymorphism (PCR-RFLP) for further case-control association analysis. Secondly the somatic mutations of DNAs from peripheral blood B lymphocytes were detected in 15 patients and 7 normal controls. Results No significant association was observed between the different alleles or genotypes of c.-347-190G>A and IgAN. The patients with different genotypes of C1GALT1C1 gene did not significantly associate with clinical manifestations, including hematuria, proteinuria, and serum creatinine of patients with IgAN. There was no somatic mutation detected in total 202 clones of 22 individuals. Conclusion The c.-347-190G>A polymorphism and the somatic mutation of encoding region of C1GALT1C1 gene were not significantly related to the genetic susceptibility to IgAN in Northern Chinese population. PMID:19778426

  6. Clearance of human native, proteinase-complexed, and proteolytically inactivated C1-inhibitor in rats.

    PubMed

    de Smet, B J; de Boer, J P; Agterberg, J; Rigter, G; Bleeker, W K; Hack, C E

    1993-01-01

    C1-inhibitor is the only known inhibitor of the classical pathway of complement and the major inhibitor of the contact pathway of coagulation. Like other serine proteinase inhibitors, C1-inhibitor can exist in three conformations, ie, the native, the proteinase-complexed, and the proteolytically inactivated form. Here we studied the plasma elimination kinetics of these three forms of human C1-inhibitor in rats. The clearance of the complexed form of C1-inhibitor appeared to be the most rapid and depended in part on the proteinase involved (observed plasma t1/2 was 20 minutes for C1s-C1-inhibitor, 32 minutes for kallikrein-C1-inhibitor, and 47 minutes for beta XIIa-C1-inhibitor), whereas that of native C1-inhibitor was the slowest (observed plasma t1/2 4.5 hours). Inactivated C1-inhibitor was cleared with an apparent plasma t1/2 of 1.6 hours. Thus, the short plasma t1/2 of complexed relative to native C1-inhibitor explains why in patients only low concentrations of C1-inhibitor complexes may be observed despite activation of the contact and/or complement systems.

  7. SLE like syndrome and functional deficiency of C1q in members of a large family.

    PubMed

    Hannema, A J; Kluin-Nelemans, J C; Hack, C E; Eerenberg-Belmer, A J; Mallée, C; van Helden, H P

    1984-01-01

    Two sisters and a brother from one family are described whose sera were deficient in haemolytic complement function. This defect was restored by addition of purified C1q. In their sera, C1q like material was found, whereas C1r and C1s were normal or increased in concentration, as were the other complement components tested. All three had suffered from glomerulonephritis during childhood. A renal biopsy in the brother recently disclosed a membranous glomerulopathy stage 1; otherwise, he is apparently healthy. In both sisters, a systemic lupus erythematosus like disease became manifest at the age of 20 and 23, respectively, resulting in the death of one of them. In the serum of these three family members, the C1q like material was antigenically deficient compared with normal C1q and had, on sucrose gradient analysis, a molecular weight of approximately 65,000 daltons. It did not bind to C1r and C1s. Binding of the dysfunctional C1q to aggregated human gammaglobulin could be demonstrated. On double immunodiffusion analysis, the abnormal C1q was identical with reduced and alkylated C1q. The possible structure of the abnormal C1q molecule is discussed.

  8. Biochemical and functional characterization of the interaction between pentraxin 3 and C1q.

    PubMed

    Nauta, Alma J; Bottazzi, Barbara; Mantovani, Alberto; Salvatori, Giovanni; Kishore, Uday; Schwaeble, Wilhelm J; Gingras, Alexandre R; Tzima, Sotiria; Vivanco, Fernando; Egido, Jesús; Tijsma, Odette; Hack, Erik C; Daha, Mohamed R; Roos, Anja

    2003-02-01

    Pentraxin 3 (PTX3) is a recently characterized member of the pentraxin family of acute-phase proteins produced during inflammation. Classical short pentraxins, C-reactive protein, and serum amyloid P component can bind to C1q and thereby activate the classical complement pathway. Since PTX3 can also bind C1q, the present study was designed to define the interaction between PTX3 and C1q and to examine the functional consequences of this interaction. A dose-dependent binding of both C1q and the C1 complex to PTX3 was observed. Experiments with recombinant globular head domains of human C1q A, B, and C chains indicated that C1q interacts with PTX3 via its globular head region. Binding of C1q to immobilized PTX3 induced activation of the classical complement pathway as assessed by C4 deposition. Furthermore, PTX3 enhanced C1q binding and complement activation on apoptotic cells. However, in the fluid-phase, pre-incubation of PTX3 with C1q resulted in inhibition of complement activation by blocking the interaction of C1q with immunoglobulins. These results indicate that PTX3 can both inhibit and activate the classical complement pathway by binding C1q, depending on the way it is presented. PTX3 may therefore be involved in the regulation of the innate immune response.

  9. 26 CFR 1.673(c)-1 - Reversionary interest after income beneficiary's death.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 26 Internal Revenue 8 2011-04-01 2011-04-01 false Reversionary interest after income beneficiary's death. 1.673(c)-1 Section 1.673(c)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE... Substantial Owners § 1.673(c)-1 Reversionary interest after income beneficiary's death. The subject matter...

  10. 26 CFR 1.673(c)-1 - Reversionary interest after income beneficiary's death.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 26 Internal Revenue 8 2012-04-01 2012-04-01 false Reversionary interest after income beneficiary's death. 1.673(c)-1 Section 1.673(c)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE... Substantial Owners § 1.673(c)-1 Reversionary interest after income beneficiary's death. The subject matter...

  11. 26 CFR 1.673(c)-1 - Reversionary interest after income beneficiary's death.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 26 Internal Revenue 8 2014-04-01 2014-04-01 false Reversionary interest after income beneficiary's death. 1.673(c)-1 Section 1.673(c)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE... Substantial Owners § 1.673(c)-1 Reversionary interest after income beneficiary's death. The subject matter...

  12. 26 CFR 1.415(c)-1 - Limitations for defined contribution plans.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 5 2010-04-01 2010-04-01 false Limitations for defined contribution plans. 1.415(c)-1 Section 1.415(c)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES Pension, Profit-Sharing, Stock Bonus Plans, Etc. § 1.415(c)-1 Limitations for defined contribution...

  13. 17 CFR 270.22c-1 - Pricing of redeemable securities for distribution, redemption and repurchase.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...) (17 CFR 270.14a-3(b))) from selling or repurchasing Trust units in a secondary market at a price based... securities for distribution, redemption and repurchase. 270.22c-1 Section 270.22c-1 Commodity and Securities... 1940 § 270.22c-1 Pricing of redeemable securities for distribution, redemption and repurchase. (a)...

  14. 17 CFR 270.22c-1 - Pricing of redeemable securities for distribution, redemption and repurchase.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...) (17 CFR 270.14a-3(b))) from selling or repurchasing Trust units in a secondary market at a price based... securities for distribution, redemption and repurchase. 270.22c-1 Section 270.22c-1 Commodity and Securities... 1940 § 270.22c-1 Pricing of redeemable securities for distribution, redemption and repurchase. (a)...

  15. 26 CFR 301.6323(c)-1 - Protection for commercial transactions financing agreements.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... financing agreements. 301.6323(c)-1 Section 301.6323(c)-1 Internal Revenue INTERNAL REVENUE SERVICE... General Provisions § 301.6323(c)-1 Protection for commercial transactions financing agreements. (a) In... financing agreement entered into before the tax lien filing, and (3) Is protected under local law against...

  16. 26 CFR 1.179C-1T - Election to expense certain refineries (temporary).

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 3 2010-04-01 2010-04-01 false Election to expense certain refineries (temporary). 1.179C-1T Section 1.179C-1T Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES Itemized Deductions for Individuals and Corporations (continued) § 1.179C-1T Election to...

  17. 26 CFR 1.503(c)-1 - Future status of organizations denied exemption.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 26 Internal Revenue 7 2014-04-01 2013-04-01 true Future status of organizations denied exemption. 1.503(c)-1 Section 1.503(c)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES (CONTINUED) Exempt Organizations § 1.503(c)-1 Future status of organizations denied exemption. (a)...

  18. 26 CFR 1.509(c)-1 - Status of organization after termination of private foundation status.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... private foundation status. 1.509(c)-1 Section 1.509(c)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES (CONTINUED) Private Foundations § 1.509(c)-1 Status of organization after termination of private foundation status. (a) In...

  19. 26 CFR 1.509(c)-1 - Status of organization after termination of private foundation status.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... private foundation status. 1.509(c)-1 Section 1.509(c)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES (CONTINUED) Private Foundations § 1.509(c)-1 Status of organization after termination of private foundation status. (a) In...

  20. 26 CFR 1.509(c)-1 - Status of organization after termination of private foundation status.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... private foundation status. 1.509(c)-1 Section 1.509(c)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES (CONTINUED) Private Foundations § 1.509(c)-1 Status of organization after termination of private foundation status. (a) In...

  1. 26 CFR 1.509(c)-1 - Status of organization after termination of private foundation status.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... private foundation status. 1.509(c)-1 Section 1.509(c)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES (CONTINUED) Private Foundations § 1.509(c)-1 Status of organization after termination of private foundation status. (a) In...

  2. Retinoic acid receptor alpha mediates growth inhibition by retinoids in rat pancreatic carcinoma DSL-6A/C1 cells.

    PubMed

    Brembeck, F H; Kaiser, A; Detjen, K; Hotz, H; Foitzik, T; Buhr, H J; Riecken, E O; Rosewicz, S

    1998-11-01

    During carcinogenesis, pancreatic acinar cells can dedifferentiate into ductal adenocarcinoma of the pancreas. DSL-6A/C1 cells represent an in vitro model of this carcinogenic sequence. This study was designed to examine the effects of retinoids on cell growth in DSL-6A/C1 cells and to characterize further the molecular mechanisms underlying the antiproliferative actions of retinoids. Treatment of DSL-6A/C1 cells with retinoids results in a time- and dose-dependent inhibition of cell growth, paralleled by a retinoid-mediated transactivation of a pTK::betaRAREx2-luciferase reporter construct transiently transfected into DSL-6A/C1 cells. Retinoid receptor expression was evaluated by reverse transcriptase polymerase chain reaction (RT-PCR) using subtype-specific primers and demonstrated expression of retinoic acid receptor alpha (RAR-alpha), RAR-beta and retinoid X receptor alpha (RXR-alpha). Using a panel of receptor subtype-specific agonists, the RAR-alpha specific agonist Ro 40-6055 was the most potent retinoid in terms of growth inhibition. Furthermore, all-trans-retinoic acid-mediated growth inhibition and transactivation was completely blocked by the RAR-alpha-specific antagonist Ro 41-5253. In summary, the RAR-alpha subtype predominantly mediates the antiproliferative effects of retinoids in DSL-6A/C1 cells. Furthermore, this cell system provides a feasible tool to study the molecular mechanisms underlying the growth inhibitory effects of retinoids in ductal pancreatic carcinoma cells derived from a primary acinar cell phenotype.

  3. Passageway: A Novel Approach to Success of Conditional Release - Principles and Constructs of the Model Residential Program for the Forensic Mentally III Patient.

    PubMed

    Melnick, Ilan

    2016-03-01

    With the advent of psychotropic medications and with the deinstitutionalization of psychiatry starting in 1968, patients were prematurely discharged from forensic state hospitals. Due to lack of resources, psychiatric forensic patients ended up in the correctional system or homeless with the reduction of psychiatric beds in forensic and civil state hospitals. Lacking proper training and medication management, the recidivism rate of this population was close to 10% for rearrest and about 35% for revocation of conditional release (CR; Manguno-Mire et al., ). A new treatment modality was created to successfully transition patients from the forensic state hospital system to the community. This article describes and analyzes the principles and constructs of Passageway, a model residential program for patients found not guilty by reason of insanity or those incompetent to proceed to CR. The CR allows for a program like Passageway to be successful in transitioning patients back into the community. This is accomplished with minimal government funding, and since 1982 has resulted in a 0% recidivism rate, for any known arrests or convictions and for recommittal of a felony, defined in the state of Florida as, "any criminal offense that is punishable under the laws of this state, or that would be punishable, if committed in this state, by death or imprisonment in a state penitentiary. " (Fla. Stat. § 775.08). Copyright © 2016 John Wiley & Sons, Ltd.

  4. Passageway: A Novel Approach to Success of Conditional Release - Principles and Constructs of the Model Residential Program for the Forensic Mentally III Patient.

    PubMed

    Melnick, Ilan

    2016-03-01

    With the advent of psychotropic medications and with the deinstitutionalization of psychiatry starting in 1968, patients were prematurely discharged from forensic state hospitals. Due to lack of resources, psychiatric forensic patients ended up in the correctional system or homeless with the reduction of psychiatric beds in forensic and civil state hospitals. Lacking proper training and medication management, the recidivism rate of this population was close to 10% for rearrest and about 35% for revocation of conditional release (CR; Manguno-Mire et al., ). A new treatment modality was created to successfully transition patients from the forensic state hospital system to the community. This article describes and analyzes the principles and constructs of Passageway, a model residential program for patients found not guilty by reason of insanity or those incompetent to proceed to CR. The CR allows for a program like Passageway to be successful in transitioning patients back into the community. This is accomplished with minimal government funding, and since 1982 has resulted in a 0% recidivism rate, for any known arrests or convictions and for recommittal of a felony, defined in the state of Florida as, "any criminal offense that is punishable under the laws of this state, or that would be punishable, if committed in this state, by death or imprisonment in a state penitentiary. " (Fla. Stat. § 775.08). Copyright © 2016 John Wiley & Sons, Ltd. PMID:27097984

  5. Glucocorticoid Receptor (NR3C1) Variants Associate with the Muscle Strength and Size Response to Resistance Training.

    PubMed

    Ash, Garrett I; Kostek, Matthew A; Lee, Harold; Angelopoulos, Theodore J; Clarkson, Priscilla M; Gordon, Paul M; Moyna, Niall M; Visich, Paul S; Zoeller, Robert F; Price, Thomas B; Devaney, Joseph M; Gordish-Dressman, Heather; Thompson, Paul D; Hoffman, Eric P; Pescatello, Linda S

    2016-01-01

    Glucocorticoid receptor (NR3C1) polymorphisms associate with obesity, muscle strength, and cortisol sensitivity. We examined associations among four NR3C1 polymorphisms and the muscle response to resistance training (RT). European-American adults (n = 602, 23.8±0.4yr) completed a 12 week unilateral arm RT program. Maximum voluntary contraction (MVC) assessed isometric strength (kg) and MRI assessed biceps size (cm2) pre- and post-resistance training. Subjects were genotyped for NR3C1 -2722G>A, -1887G>A, -1017T>C, and +363A>G. Men carrying the -2722G allele gained less relative MVC (17.3±1.2vs33.5±6.1%) (p = 0.010) than AA homozygotes; men with -1887GG gained greater relative MVC than A allele carriers (19.6±1.4vs13.2±2.3%) (p = 0.016). Women carrying the -1017T allele gained greater relative size (18.7±0.5vs16.1±0.9%) (p = 0.016) than CC homozygotes. We found sex-specific NR3C1 associations with the muscle strength and size response to RT. Future studies should investigate whether these associations are partially explained by cortisol's actions in muscle tissue as they interact with sex differences in cortisol production. PMID:26821164

  6. Glucocorticoid Receptor (NR3C1) Variants Associate with the Muscle Strength and Size Response to Resistance Training.

    PubMed

    Ash, Garrett I; Kostek, Matthew A; Lee, Harold; Angelopoulos, Theodore J; Clarkson, Priscilla M; Gordon, Paul M; Moyna, Niall M; Visich, Paul S; Zoeller, Robert F; Price, Thomas B; Devaney, Joseph M; Gordish-Dressman, Heather; Thompson, Paul D; Hoffman, Eric P; Pescatello, Linda S

    2016-01-01

    Glucocorticoid receptor (NR3C1) polymorphisms associate with obesity, muscle strength, and cortisol sensitivity. We examined associations among four NR3C1 polymorphisms and the muscle response to resistance training (RT). European-American adults (n = 602, 23.8±0.4yr) completed a 12 week unilateral arm RT program. Maximum voluntary contraction (MVC) assessed isometric strength (kg) and MRI assessed biceps size (cm2) pre- and post-resistance training. Subjects were genotyped for NR3C1 -2722G>A, -1887G>A, -1017T>C, and +363A>G. Men carrying the -2722G allele gained less relative MVC (17.3±1.2vs33.5±6.1%) (p = 0.010) than AA homozygotes; men with -1887GG gained greater relative MVC than A allele carriers (19.6±1.4vs13.2±2.3%) (p = 0.016). Women carrying the -1017T allele gained greater relative size (18.7±0.5vs16.1±0.9%) (p = 0.016) than CC homozygotes. We found sex-specific NR3C1 associations with the muscle strength and size response to RT. Future studies should investigate whether these associations are partially explained by cortisol's actions in muscle tissue as they interact with sex differences in cortisol production.

  7. Glucocorticoid Receptor (NR3C1) Variants Associate with the Muscle Strength and Size Response to Resistance Training

    PubMed Central

    Ash, Garrett I.; Kostek, Matthew A.; Lee, Harold; Angelopoulos, Theodore J.; Gordon, Paul M.; Moyna, Niall M.; Visich, Paul S.; Zoeller, Robert F.; Price, Thomas B.; Devaney, Joseph M.; Gordish-Dressman, Heather; Thompson, Paul D.; Hoffman, Eric P.; Pescatello, Linda S.

    2016-01-01

    Glucocorticoid receptor (NR3C1) polymorphisms associate with obesity, muscle strength, and cortisol sensitivity. We examined associations among four NR3C1 polymorphisms and the muscle response to resistance training (RT). European-American adults (n = 602, 23.8±0.4yr) completed a 12 week unilateral arm RT program. Maximum voluntary contraction (MVC) assessed isometric strength (kg) and MRI assessed biceps size (cm2) pre- and post-resistance training. Subjects were genotyped for NR3C1 -2722G>A, -1887G>A, -1017T>C, and +363A>G. Men carrying the -2722G allele gained less relative MVC (17.3±1.2vs33.5±6.1%) (p = 0.010) than AA homozygotes; men with -1887GG gained greater relative MVC than A allele carriers (19.6±1.4vs13.2±2.3%) (p = 0.016). Women carrying the -1017T allele gained greater relative size (18.7±0.5vs16.1±0.9%) (p = 0.016) than CC homozygotes. We found sex-specific NR3C1 associations with the muscle strength and size response to RT. Future studies should investigate whether these associations are partially explained by cortisol’s actions in muscle tissue as they interact with sex differences in cortisol production. PMID:26821164

  8. Characterization of recombinant human C1 inhibitor secreted in milk of transgenic rabbits.

    PubMed

    van Veen, Harrie A; Koiter, Jaco; Vogelezang, Carla J M; van Wessel, Noucha; van Dam, Tijtje; Velterop, Ingeborg; van Houdt, Kristina; Kupers, Luc; Horbach, Danielle; Salaheddine, Mourad; Nuijens, Jan H; Mannesse, Maurice L M

    2012-12-31

    C1 inhibitor (C1INH) is a single-chain glycoprotein that inhibits activation of the contact system of coagulation and the complement system. C1INH isolated from human blood plasma (pd-hC1INH) is used for the management of hereditary angioedema (HAE), a disease caused by heterozygous deficiency of C1INH, and is a promise for treatment of ischemia-reperfusion injuries like acute myocardial or cerebral infarction. To obtain large quantities of C1INH, recombinant human C1INH (rhC1INH) was expressed in the milk of transgenic rabbits (12 g/l) harboring genomic human C1INH sequences fused to 5' bovine αS(1) casein promoter sequences. Recombinant hC1INH was isolated from milk to a specific activity of 6.1 U/mg and a purity of 99%; by size-exclusion chromatography the 1% impurities consisted of multimers and N-terminal cleaved C1INH species. Mass spectrometric analysis of purified rhC1INH revealed a relative molecular mass (M(r)) of 67,200. Differences in M(r) on SDS PAGE and mass spectrometric analysis between rhC1INH and pd-hC1INH are explained by differential glycosylation (calculated carbohydrate contents of 21% and 28%, respectively), since protein sequencing analysis of rhC1INH revealed intact N- and C-termini. Host-related impurity analysis by ELISA revealed trace amounts of rabbit protein (approximately 10 ppm) in purified batches, but not endogenous rabbit C1INH. The kinetics of inhibition of the target proteases C1s, Factor XIIa, kallikrein and Factor XIa by rhC1INH and pd-hC1INH, indicated comparable inhibitory potency and specificity. Recently, rhC1INH (Ruconest(®)) has been approved by the European Medicines Agency for the treatment of acute attacks of HAE. PMID:22995741

  9. Effect of reactive site loop elongation on the inhibitory activity of C1-inhibitor.

    PubMed

    Bos, Ineke G A; Lubbers, Yvonne T P; Eldering, Eric; Abrahams, Jan Pieter; Hack, C Erik

    2004-06-01

    The serine protease inhibitor C1-Inhibitor (C1-Inh) inhibits several complement- and contact-system proteases, which play an important role in inflammation. C1-Inh has a short reactive site loop (RSL) compared to other serpins. RSL length determines the inhibitory activity of serpins. We investigated the effect of RSL elongation on inhibitory activity of C1-Inh by insertion of one or two alanine residues in the RSL. One of five mutants had an increased association rate with kallikrein, but was nevertheless a poor inhibitor because of a simultaneous high stoichiometry of inhibition (>10). The association rate of the other variants was lower than that of wild-type C1-Inh. These data suggest that the relatively weak inhibitory activity of C1-Inh is not the result of its short RSL. The short RSL of C1-Inh has, surprisingly, the optimal length for inhibition.

  10. Preface: phys. stat. sol. (c) 1/7

    NASA Astrophysics Data System (ADS)

    Cheikhrouhou, Abdelwaheb

    2004-05-01

    Physics Laboratory, Sciences Faculty of Sfax (Tunisia) and the Co-Chairmen were Professor Sami Mahmood, Dean of Sciences at Yarmouk University (Jordan) and Professor Mohamed Akhavan from the Sharif University of Technology (Iran). The four-day conference consisted of several oral and poster sessions, followed by social programs in the evenings. The success of the event could be measured during the closing session on the last day, when several delegates emphasized the high-quality science that had been evident at the conference. A post conference three-day tour to the south of Tunisia (Matmata, Douz City: the gate of desert and the mountains oasis: Tamerza, Mides and Chebika) was also arranged. The conference was generously sponsored by: - The Tunisian Ministry of High Education, Scientific Research and Technology - The Tunisian Secretary of State for Scientific Research and Technology - The Tunisian National Office of Tourism - The Abdus Salam International Centre for Theoretical Physics (ICTP) - French Institute for Cooperation in Tunisia - Tunisian-Italian Scientific Partnership - British Gas - Tunisian Society for Electricity and Gas - Imex Olive Oil -Confiserie TRIKI Le Moulin The next MSM conference in 2005 will be held in Morocco.

  11. Skeletally Diverse Synthesis of Innovative [2,1-c]-1,4-Oxazepine and [1,4]-Quinoxaline Systems.

    PubMed

    Lee, Chia-Hsin; Wu, Wen-Chun; Dangate, Prasad S; Shen, Li-Ching; Chung, Wen-Sheng; Sun, Chung-Ming

    2015-10-12

    An efficient, innovative synthesis of [2,1-c]-1, 4-oxazepine and [1,4]-quinoxaline heterocycles along with the embodied pyrimido-pyrrolo motifs was established. Initially, the pyrrole ring was installed using microwave irradiation through an intramolecular base-catalyzed cyclization between acetyl bromomethyl pyrimidine dione and o-amino phenyl methanol or o-phenylenediamine methyl benzoates. Furthermore, oxazepine, and quinoxaline scaffolds were constructed by an acid-catalyzed condensation with a variety of aldehydes by an unconventional Pictet-Spengler reaction strategy. An important aspect of this work is to build novel heterocyclic ring systems with potential medicinal interest.

  12. Skeletally Diverse Synthesis of Innovative [2,1-c]-1,4-Oxazepine and [1,4]-Quinoxaline Systems.

    PubMed

    Lee, Chia-Hsin; Wu, Wen-Chun; Dangate, Prasad S; Shen, Li-Ching; Chung, Wen-Sheng; Sun, Chung-Ming

    2015-10-12

    An efficient, innovative synthesis of [2,1-c]-1, 4-oxazepine and [1,4]-quinoxaline heterocycles along with the embodied pyrimido-pyrrolo motifs was established. Initially, the pyrrole ring was installed using microwave irradiation through an intramolecular base-catalyzed cyclization between acetyl bromomethyl pyrimidine dione and o-amino phenyl methanol or o-phenylenediamine methyl benzoates. Furthermore, oxazepine, and quinoxaline scaffolds were constructed by an acid-catalyzed condensation with a variety of aldehydes by an unconventional Pictet-Spengler reaction strategy. An important aspect of this work is to build novel heterocyclic ring systems with potential medicinal interest. PMID:26379108

  13. Construction & Basic Skills.

    ERIC Educational Resources Information Center

    BCEL Newsletter for the Business and Literacy Communities, 1991

    1991-01-01

    Basic skills education has become a pressing need in the construction industry as jobs become more complex and fewer workers have needed skills. However, the construction industry lags in spending on training for entry-level workers. The Home Builders Institute (HBI) is testing a pilot basic skills program that it hopes will prove useful to the…

  14. CT-based morphometric analysis of C1 laminar dimensions: C1 translaminar screw fixation is a feasible technique for salvage of atlantoaxial fusions

    PubMed Central

    Yew, Andrew; Lu, Derek; Lu, Daniel C.

    2015-01-01

    Background: Translaminar screw fixation has become an alternative in the fixation of the axial and subaxial cervical spine. We report utilization of this approach in the atlas as a salvage technique for atlantoaxial stabilization when C1 lateral mass screws are precluded. To assess the feasibility of translaminar fixation at the atlas, we have characterized the dimensions of the C1 lamina in the general adult population using computed tomography (CT)-based morphometry. Methods: A 46-year-old male with symptomatic atlantoaxial instability secondary to os odontoideum underwent bilateral C1 and C2 translaminar screw/rod fixation as C1 lateral mass fixation was precluded by an anomalous vertebral artery. The follow-up evaluation 2½ years postoperatively revealed an asymptomatic patient without recurrent neck/shoulder pain or clinical signs of instability. To better assess the feasibility of utilizing this approach in the general population, we retrospectively analyzed 502 consecutive cervical CT scans performed over a 3-month period in patients aged over 18 years at a single institution. Measurements of C1 bicortical diameter, bilateral laminar length, height, and angulation were performed. Laminar and screw dimensions were compared to assess instrumentation feasibility. Results: Review of CT imaging found that 75.9% of C1 lamina had a sufficient bicortical diameter, and 63.7% of C1 lamina had sufficient height to accept bilateral translaminar screw placement. Conclusions: CT-based measurement of atlas morphology in the general population revealed that a majority of C1 lamina had sufficient dimensions to accept translaminar screw placement. Although these screws appear to be a feasible alternative when lateral mass screws are precluded, further research is required to determine if they provide comparable fixation strength versus traditional instrumentation methods. PMID:26005585

  15. Biodegradation and adsorption of C1- and C2-phenanthrenes and C1- and C2-dibenzothiophenes in the presence of clay minerals: effect on forensic diagnostic ratios.

    PubMed

    Ugochukwu, Uzochukwu C; Head, Ian M; Manning, David A C

    2014-07-01

    The impact of modified montmorillonites on adsorption and biodegradation of crude oil C1-phenanthrenes, C1-dibenzothiophenes, C2-phenanthrenes and C2-dibenzothiophenes was investigated in aqueous clay/oil microcosm experiments with a hydrocarbon degrading microorganism community. Consequently, the effect on C1-dibenzothiophenes/C1-phenanthrenes, C2-dibenzothiophenes/C2-phenanthrenes, 2+3-methyldibenzothiophene/4-methyldibenzothiophene and 1-methyldibenzothiophene/4-methyldibenzothiophene ratios commonly used as diagnostic ratios for oil forensic studies was evaluated. The clay mineral samples were treated to produce acid activated montmorillonite, organomontmorillonite and homoionic montmorillonite which were used in this study. The different clay minerals (modified and unmodified) showed varied degrees of biodegradation and adsorption of the C1-phenanthrenes, C1-dibenzothiophenes, C2-phenanthrenes and C2-dibenzothiophenes. The study indicated that as opposed to biodegradation, adsorption has no effect on the diagnostic ratios. Among the diagnostic ratios reviewed, only C2-dibenzothiophenes/C2-phenanthrenes ratio was neither affected by adsorption nor biodegradation making this ratio very useful in forensic studies of oil spills and oil-oil correlation.

  16. Designing of skull defect implants using C1 rational cubic Bezier and offset curves

    NASA Astrophysics Data System (ADS)

    Mohamed, Najihah; Majid, Ahmad Abd; Piah, Abd Rahni Mt; Rajion, Zainul Ahmad

    2015-05-01

    Some of the reasons to construct skull implant are due to head trauma after an accident or an injury or an infection or because of tumor invasion or when autogenous bone is not suitable for replacement after a decompressive craniectomy (DC). The main objective of our study is to develop a simple method to redesign missing parts of the skull. The procedure begins with segmentation, data approximation, and estimation process of the outer wall by a C1 continuous curve. Its offset curve is used to generate the inner wall. A metaheuristic algorithm, called harmony search (HS) is a derivative-free real parameter optimization algorithm inspired from the musical improvisation process of searching for a perfect state of harmony. In this study, data approximation by a rational cubic Bézier function uses HS to optimize position of middle points and value of the weights. All the phases contribute significantly in making our proposed technique automated. Graphical examples of several postoperative skulls are displayed to show the effectiveness of our proposed method.

  17. Functional C1-inhibitor diagnostics in hereditary angioedema: assay evaluation and recommendations.

    PubMed

    Wagenaar-Bos, Ineke G A; Drouet, Christian; Aygören-Pursun, Emel; Bork, Konrad; Bucher, Christoph; Bygum, Anette; Farkas, Henriette; Fust, George; Gregorek, Hanna; Hack, C Erik; Hickey, Alaco; Joller-Jemelka, Helen I; Kapusta, Maria; Kreuz, Wolfhart; Longhurst, Hilary; Lopez-Trascasa, Margarita; Madalinski, Kazimierz; Naskalski, Jerzy; Nieuwenhuys, Ed; Ponard, Denise; Truedsson, Lennart; Varga, Lilian; Nielsen, Erik Waage; Wagner, Eric; Zingale, Lorenza; Cicardi, Marco; van Ham, S Marieke

    2008-09-30

    Hereditary angioedema (HAE) is an autosomal dominant disease characterized by recurrent episodes of potentially life-threatening angioedema. The most widespread underlying genetic deficiency is a heterozygous deficiency of the serine protease inhibitor C1 esterase inhibitor (C1-Inh). In addition to low C4 levels, the most important laboratory parameter for correct diagnosis of HAE or angioedema due to acquired C1-Inh deficiency is reduced C1-Inh function (fC1-Inh). No direct recommendations about the assays for fC1-Inh or sample handling conditions are available, although this would prove especially useful when a laboratory first starts to offer assays on fC1-Inh for HAE diagnosis. In the present study we evaluated the performance of fC1-Inh assays in the 15 different laboratories that are specialised in HAE diagnostics and assessed inter-laboratory variation with each laboratory using their own assays and standards. A double-blind survey was conducted using plasma/serum samples from healthy donors and HAE patients and the uniformity of HAE diagnosis was evaluated. It can be concluded that the diagnosis of fC1-Inh deficiency was made correctly in most cases in this survey. We can recommend the chromogenic assay for the determination of fC1-Inh, while the complex ELISA needs further investigation.

  18. Gonococcal infection in a nonhuman host is determined by human complement C1q.

    PubMed

    Nowicki, S; Martens, M G; Nowicki, B J

    1995-12-01

    Human C1q displayed a dose-dependent protection of gonococcal cells (GC) from the bactericidal effect of newborn rat serum. All rat pups injected with C1q-preincubated GC developed bacteremia, while none of the animals injected with GC only were infected. After clearance of bacteremia at day 6, live GC could still be recovered from tested organs, including the liver. Preincubation of GC with higher concentrations of C1q was associated with increased morbidity. In contrast to human serum as a source of C1q, rat, rabbit, and mouse sera did not increase the in vivo virulence of Neisseria gonorrhoeae. C1q-deficient human serum, heat-inactivated C1q or human serum, type IV collagen, and complement C3 were inefficient in inducing infection. Experimental infection by C1q-preincubated GC was inhibited by anti-C1q antibodies in a dose-dependent fashion, demonstrating a causal effect of C1q function. This report demonstrates the novel finding that human C1q, a component of the human immune system with a general function for elimination of infection, may increase GC virulence and result in the development of disseminated infection in a nonhuman host.

  19. Gonococcal infection in a nonhuman host is determined by human complement C1q.

    PubMed Central

    Nowicki, S; Martens, M G; Nowicki, B J

    1995-01-01

    Human C1q displayed a dose-dependent protection of gonococcal cells (GC) from the bactericidal effect of newborn rat serum. All rat pups injected with C1q-preincubated GC developed bacteremia, while none of the animals injected with GC only were infected. After clearance of bacteremia at day 6, live GC could still be recovered from tested organs, including the liver. Preincubation of GC with higher concentrations of C1q was associated with increased morbidity. In contrast to human serum as a source of C1q, rat, rabbit, and mouse sera did not increase the in vivo virulence of Neisseria gonorrhoeae. C1q-deficient human serum, heat-inactivated C1q or human serum, type IV collagen, and complement C3 were inefficient in inducing infection. Experimental infection by C1q-preincubated GC was inhibited by anti-C1q antibodies in a dose-dependent fashion, demonstrating a causal effect of C1q function. This report demonstrates the novel finding that human C1q, a component of the human immune system with a general function for elimination of infection, may increase GC virulence and result in the development of disseminated infection in a nonhuman host. PMID:7591137

  20. The importance of polarizability: comparison of models of carbon disulphide in the ionic liquids [C1C1im][NTf2] and [C4C1im][NTf2].

    PubMed

    Lynden-Bell, Ruth M; Quitevis, Edward L

    2016-06-28

    The local environment of CS2 and in solution in two ionic liquids ([C1C1im][NTf2] and [C4C1im][NTf2]) are investigated by atomistic simulation and compared with that in neat CS2. The intermolecular vibrational densities of states of CS2 are calculated and compared with experimental OHD-RIKES spectra. The fair agreement of the results from solutions but poor agreement of the results from neat CS2 suggest that while collective effects are unimportant in solutions, they have a major effect on the OHD-RIKES spectrum of neat CS2. Comparing polarizable and unpolarizable models for CS2 emphasizes the importance of polarizability in determining local structure.

  1. Aviation Safety Modeling and Simulation (ASMM) Propulsion Fleet Modeling: A Tool for Semi-Automatic Construction of CORBA-based Applications from Legacy Fortran Programs

    NASA Technical Reports Server (NTRS)

    Sang, Janche

    2003-01-01

    Within NASA's Aviation Safety Program, NASA GRC participates in the Modeling and Simulation Project called ASMM. NASA GRC s focus is to characterize the propulsion systems performance from a fleet management and maintenance perspective by modeling and through simulation predict the characteristics of two classes of commercial engines (CFM56 and GE90). In prior years, the High Performance Computing and Communication (HPCC) program funded, NASA Glenn in developing a large scale, detailed simulations for the analysis and design of aircraft engines called the Numerical Propulsion System Simulation (NPSS). Three major aspects of this modeling included the integration of different engine components, coupling of multiple disciplines, and engine component zooming at appropriate level fidelity, require relatively tight coupling of different analysis codes. Most of these codes in aerodynamics and solid mechanics are written in Fortran. Refitting these legacy Fortran codes with distributed objects can increase these codes reusability. Aviation Safety s modeling and simulation use in characterizing fleet management has similar needs. The modeling and simulation of these propulsion systems use existing Fortran and C codes that are instrumental in determining the performance of the fleet. The research centers on building a CORBA-based development environment for programmers to easily wrap and couple legacy Fortran codes. This environment consists of a C++ wrapper library to hide the details of CORBA and an efficient remote variable scheme to facilitate data exchange between the client and the server model. Additionally, a Web Service model should also be constructed for evaluation of this technology s use over the next two- three years.

  2. C1 Chemistry for the Production of Ultra-Clean Liquid Transportation Fuels and Hydrogen

    SciTech Connect

    Gerald P. Huffman

    2006-03-30

    Professors and graduate students from five universities--the University of Kentucky, University of Pittsburgh, University of Utah, West Virginia University, and Auburn University--are collaborating in a research program to develop C1 chemistry processes to produce ultra-clean liquid transportation fuels and hydrogen, the zero-emissions transportation fuel of the future. The feedstocks contain one carbon atom per molecular unit. They include synthesis gas (syngas), a mixture of carbon monoxide and hydrogen produced by coal gasification or reforming of natural gas, methane, methanol, carbon dioxide, and carbon monoxide. An important objective is to develop C1 technology for the production of liquid transportation fuel and hydrogen from domestically plentiful resources such as coal, coalbed methane, and hydrocarbon gases and liquids produced from coal. An Advisory Board with representatives from Chevron-Texaco, Eastman Chemical, Conoco-Phillips, the Air Force Research Laboratory, the U.S. Army National Automotive Center, and Tier Associates provides guidance on the practicality of the research. The current report summarizes the results obtained in this program during the period October 1, 2002 through March 31, 2006. The results are presented in detailed reports on 16 research projects headed by professors at each of the five CFFS Universities and an Executive Summary. Some of the highlights from these results are: (1) Small ({approx}1%) additions of acetylene or other alkynes to the Fischer-Tropsch (F-T) reaction increases its yield, causes chain initiation, and promotes oxygenate formation. (2) The addition of Mo to Fe-Cu-K/AC F-T catalysts improves catalyst lifetime and activity. (3) The use of gas phase deposition to place highly dispersed metal catalysts on silica or ceria aerogels offers promise for both the F-T and the water-gas shift WGS reactions. (4) Improved activity and selectivity are exhibited by Co F-T catalysts in supercritical hexane. (5) Binary Fe

  3. C1 CHEMISTRY FOR THE PRODUCTION OF ULTRA-CLEAN LIQUID TRANSPORTATION FUELS AND HYDROGEN

    SciTech Connect

    Gerald P. Huffman

    2003-09-30

    The Consortium for Fossil Fuel Science (CFFS) is a research consortium with participants from the University of Kentucky, University of Pittsburgh, University of Utah, West Virginia University, and Auburn University. The CFFS is conducting a research program to develop C1 chemistry technology for the production of clean transportation fuel from resources such as coal and natural gas, which are more plentiful domestically than petroleum. The processes under development will convert feedstocks containing one carbon atom per molecular unit into ultra clean liquid transportation fuels (gasoline, diesel, and jet fuel) and hydrogen, which many believe will be the transportation fuel of the future. These feedstocks include synthesis gas, a mixture of carbon monoxide and hydrogen produced by coal gasification or reforming of natural gas, methane, methanol, carbon dioxide, and carbon monoxide. Some highlights of the results obtained during the first year of the current research contract are summarized as: (1) Terminal alkynes are an effective chain initiator for Fischer-Tropsch (FT) reactions, producing normal paraffins with C numbers {ge} to that of the added alkyne. (2) Significant improvement in the product distribution towards heavier hydrocarbons (C{sub 5} to C{sub 19}) was achieved in supercritical fluid (SCF) FT reactions compared to that of gas-phase reactions. (3) Xerogel and aerogel silica supported cobalt catalysts were successfully employed for FT synthesis. Selectivity for diesel range products increased with increasing Co content. (4) Silicoaluminophosphate (SAPO) molecular sieve catalysts have been developed for methanol to olefin conversion, producing value-added products such as ethylene and propylene. (5) Hybrid Pt-promoted tungstated and sulfated zirconia catalysts are very effective in cracking n-C{sub 36} to jet and diesel fuel; these catalysts will be tested for cracking of FT wax. (6) Methane, ethane, and propane are readily decomposed to pure

  4. C1 inhibitor hinge region mutations produce dysfunction by different mechanisms.

    PubMed

    Davis, A E; Aulak, K; Parad, R B; Stecklein, H P; Eldering, E; Hack, C E; Kramer, J; Strunk, R C; Bissler, J; Rosen, F S

    1992-08-01

    Heterozygosity for a mutant dysfunctional C1 inhibitor protein, a member of the serine proteinase inhibitor (serpin) superfamily, results in type II hereditary angioneurotic oedema. We identified a "hinge" region mutation in C1 inhibitor with a Val to Glu replacement at P14 Val-432. Recombinant C1 inhibitors P10 Ala-->Thr and P14Val-->Glu did not form stable complexes with fluid phase C1s or kallikrein. The P14 Val-->Glu mutant, however, was cleaved to a 96K form by C1s, while the P10 Ala-->Thr mutant was not. The recombinant P10 mutant also did not complex with C1s, kallikrein or beta-factor Xlla-Sepharose. The two mutations, therefore, result in dysfunction by different mechanisms: in one (P14 Val-->Glu), the inhibitor is converted to a substrate, while in the other (P10 Ala-->Thr), interaction with target protease is blocked.

  5. C1 inhibitor treatment improves host defense in pneumococcal meningitis in rats and mice.

    PubMed

    Zwijnenburg, Petra J G; van der Poll, Tom; Florquin, Sandrine; Polfliet, Machteld M J; van den Berg, Timo K; Dijkstra, Christine D; Roord, John J; Hack, C Erik; van Furth, A Marceline

    2007-07-01

    In spite of antibiotic treatment, pneumococcal meningitis continues to be associated with significant morbidity and mortality. The complement system is a key component of innate immunity against invading pathogens. However, activation of complement is also involved in tissue damage, and complement inhibition by C1 inhibitor (C1-inh) is beneficial in animal models of endotoxemia and sepsis. In the present study, we demonstrate classical pathway complement activation during pneumococcal meningitis in rats. We also evaluate the effect of C1-inh treatment on clinical illness, bacterial clearance, and inflammatory responses in rats and mice with pneumococcal meningitis. C1-inh treatment was associated with reduced clinical illness, a less-pronounced inflammatory infiltrate around the meninges, and lower brain levels of proinflammatory cytokines and chemokines. C1-inh treatment increased bacterial clearance, possibly through an up-regulation of CR3. Hence, C1-inh may be a useful agent in the treatment of pneumococcal meningitis.

  6. New insight into the effects of heparinoids on complement inhibition by C1-inhibitor.

    PubMed

    Poppelaars, F; Damman, J; de Vrij, E L; Burgerhof, J G M; Saye, J; Daha, M R; Leuvenink, H G; Uknis, M E; Seelen, M A J

    2016-06-01

    Complement activation is of major importance in numerous pathological conditions. Therefore, targeted complement inhibition is a promising therapeutic strategy. C1-esterase inhibitor (C1-INH) controls activation of the classical pathway (CP) and the lectin pathway (LP). However, conflicting data exist on inhibition of the alternative pathway (AP) by C1-INH. The inhibitory capacity of C1-INH for the CP is potentiated by heparin and other glycosaminoglycans, but no data exist for the LP and AP. The current study investigates the effects of C1-INH in the presence or absence of different clinically used heparinoids on the CP, LP and AP. Furthermore, the combined effects of heparinoids and C1-INH on coagulation were investigated. C1-INH, heparinoids or combinations were analysed in a dose-dependent fashion in the presence of pooled serum. Functional complement activities were measured simultaneously using the Wielisa(®) -kit. The activated partial thrombin time was determined using an automated coagulation analyser. The results showed that all three complement pathways were inhibited significantly by C1-INH or heparinoids. Next to their individual effects on complement activation, heparinoids also enhanced the inhibitory capacity of C1-INH significantly on the CP and LP. For the AP, significant potentiation of C1-INH by heparinoids was found; however, this was restricted to certain concentration ranges. At low concentrations the effect on blood coagulation by combining heparinoids with C1-INH was minimal. In conclusion, our study shows significant potentiating effects of heparinoids on the inhibition of all complement pathways by C1-INH. Therefore, their combined use is a promising and a potentially cost-effective treatment option for complement-mediated diseases.

  7. Home treatment of attacks with conestat alfa in hereditary angioedema due to C1-inhibitor deficiency.

    PubMed

    Farkas, Henriette; Csuka, Dorottya; Veszeli, Nóra; Zotter, Zsuzsanna; Szabó, Erika; Varga, Lilian

    2014-01-01

    Conestat alfa, a recombinant human C1 inhibitor (rhC1-INH) is a novel therapeutic option for the acute treatment of hereditary angioedema due to C1-INH (HAE-C1-INH) deficiency. Our aim was to investigate the efficacy and safety profile of conestat alfa in patients with HAE-C1-INH, under real-life conditions. We analyzed 65 edematous episodes requiring acute treatment and occurring in two female HAE-C1-INH patients. The patients were treated at home with rhC1-INH per occasion. They recorded the time of rhC1-INH administration, the time to the onset of improvement, and time to the complete resolution of symptoms, as well as the side effects. Symptom severity and patient satisfaction were measured with a visual analog scale (VAS). Thirty-three HAE attacks occurred in submucosal tissue, 17 in subcutaneous tissue, and 15 had mixed locations. After the administration of rhC1-INH, clinical symptoms improved within 0.50 (0.17-4.50 hours) hours and resolved completely within 9.00 (1.67-58.75 hours) hours. The time between the onset of the attack and the administration of rhC1-INH was correlated with the time when the symptoms stopped worsening (R = 0.3212; p = 0.0096) and the time to complete resolution of the symptoms (R = 0.4774; p < 0.0001). The time to response to the drug differed with attack location. The efficacy and safety of rhC1-INH persisted after repeated use. None of the patients experienced a recurrence of the HAE attack or drug-related systemic adverse events. The mean VAS score of patient satisfaction was 93.14. Home treatment with rhC1-INH was an effective and well-tolerated therapy for all types of HAE attacks.

  8. C1-esterase inhibitor treatment: preclinical safety aspects on the potential prothrombotic risk.

    PubMed

    Schürmann, Daniel; Herzog, Eva; Raquet, Elmar; Nolte, Marc W; May, Frauke; Müller-Cohrs, Jochen; Björkqvist, Jenny; Dickneite, Gerhard; Pragst, Ingo

    2014-11-01

    Human plasma-derived C1-esterase inhibitor (C1-INH) is an efficacious and safe treatment for hereditary angioedema. However, thrombotic events in subjects treated with C1-INH at recommended or off-label, high doses have been reported. In this study, we addressed the potential prothrombotic risk of C1-INH treatment in high doses using a non-clinical rabbit model. Following intravenous infusion of C1-INH to rabbits at doses up to 800 IU/kg, the exposure and the pharmacodynamic efficacy of C1-INH in rabbits were confirmed by activity measurements of C1-esterase, and coagulation factors XIa and XIIa, respectively. Potential prothrombotic effects were assessed following induction of venous and arterial thrombosis using in vivo models of venous and arterial stasis, complemented by various in vitro assays of coagulation markers. Administration of C1-INH at doses up to 800 IU/kg did not potentiate thrombus formation during venous stasis. In contrast, inhibition of arterial occlusion was observed upon C1-INH administration when compared with isotonic saline treatment, indicating antithrombotic rather than prothrombotic activity of high dose C1-INH treatment in vivo. This was further confirmed in vitro by decreased thrombin generation, increased activated partial thromboplastin time, clotting time and clot formation time, and inhibition of platelet aggregation. No relevant changes in fibrinolysis or in the levels of thrombin-antithrombin complexes, and prothrombin fragment 1+2 were observed upon high dose C1-INH treatment. The data suggest that treatment of healthy rabbits with high doses of C1-INH could potentially inhibit coagulation and thrombus formation rather than induce a prothrombotic risk.

  9. Recombinant human C1 esterase inhibitor in the management of hereditary angioedema.

    PubMed

    Riedl, Marc

    2015-07-01

    Hereditary angioedema (HAE), a rare autosomal dominant genetic disorder, is caused by a deficiency in functional C1 esterase inhibitor (C1-INH). This potentially life-threatening condition manifests as recurrent attacks of subcutaneous and submucosal swelling of the skin, gastrointestinal tract and larynx. The management of HAE includes treatment of acute episodes, short-term prophylaxis in preparation for exposure to known triggers and long-term prophylaxis to decrease the incidence and severity of HAE attacks. Four products are approved in the USA for the treatment of acute attacks of HAE, including one human plasma-derived C1-INH therapy, a recombinant human C1-INH product (rhC1-INH), a plasma kallikrein inhibitor and a bradykinin B2 receptor antagonist. In addition, one human plasma-derived C1-INH therapy and danazol are approved for prophylaxis of HAE attacks. rhC1-INH, extracted from the milk of transgenic rabbits, is a glycoprotein of 478 amino acids with an identical amino acid sequence to the endogenous human C1-INH protein. Population pharmacokinetic analysis of rhC1-INH supports an intravenous dosing strategy of 50 U/kg (maximum 4200 U). The safety and efficacy of rhC1-INH in the treatment of acute attacks in patients with HAE were demonstrated in three randomized, double-blind, placebo-controlled studies and two open-label extension studies. In a pilot prophylaxis study, weekly administration of rhC1-INH 50 U/kg for 8 weeks reduced the incidence of HAE attacks and was well tolerated. Administration of rhC1-INH has not been associated with the development of anti-drug antibodies or antibodies to anti-host-related impurities.

  10. New insight into the effects of heparinoids on complement inhibition by C1-inhibitor.

    PubMed

    Poppelaars, F; Damman, J; de Vrij, E L; Burgerhof, J G M; Saye, J; Daha, M R; Leuvenink, H G; Uknis, M E; Seelen, M A J

    2016-06-01

    Complement activation is of major importance in numerous pathological conditions. Therefore, targeted complement inhibition is a promising therapeutic strategy. C1-esterase inhibitor (C1-INH) controls activation of the classical pathway (CP) and the lectin pathway (LP). However, conflicting data exist on inhibition of the alternative pathway (AP) by C1-INH. The inhibitory capacity of C1-INH for the CP is potentiated by heparin and other glycosaminoglycans, but no data exist for the LP and AP. The current study investigates the effects of C1-INH in the presence or absence of different clinically used heparinoids on the CP, LP and AP. Furthermore, the combined effects of heparinoids and C1-INH on coagulation were investigated. C1-INH, heparinoids or combinations were analysed in a dose-dependent fashion in the presence of pooled serum. Functional complement activities were measured simultaneously using the Wielisa(®) -kit. The activated partial thrombin time was determined using an automated coagulation analyser. The results showed that all three complement pathways were inhibited significantly by C1-INH or heparinoids. Next to their individual effects on complement activation, heparinoids also enhanced the inhibitory capacity of C1-INH significantly on the CP and LP. For the AP, significant potentiation of C1-INH by heparinoids was found; however, this was restricted to certain concentration ranges. At low concentrations the effect on blood coagulation by combining heparinoids with C1-INH was minimal. In conclusion, our study shows significant potentiating effects of heparinoids on the inhibition of all complement pathways by C1-INH. Therefore, their combined use is a promising and a potentially cost-effective treatment option for complement-mediated diseases. PMID:26874675

  11. C 1s Near Edge X-ray Absorption Fine Structure (NEXAFS) of substituted benzoic acids: a theoretical and experimental study

    SciTech Connect

    Baldea,I.; Schimmelpfennig, B.; Plaschke, M.; Rothe, J.; Schirmer, J.; Trofimov, A.; Fanghaenel, T.

    2007-01-01

    Ab initio calculations are performed to explain the discrete transitions in experimental C 1s-NEXAFS (near edge X-ray absorption fine structure) spectra of various benzoic acid derivates. Transition energies and oscillator strengths of the contributing C 1s-{pi}* excitations are computed using the ADC(2) (second-order algebraic-diagrammatic construction) method. This method is demonstrated to be well suited for the finite electronic systems represented by these simple organic acids. There is good agreement between experiment and theory reproducing all the relevant spectral features. Some transitions can only be assigned based on a theoretical foundation. Remaining discrepancies between experimental and computed spectra are discussed.

  12. Acquired C1 esterase inhibitor deficiency in lymphomas: prevalence, symptoms, and response to treatment.

    PubMed

    Bekos, Christine; Perkmann, Thomas; Krauth, Maria; Raderer, Markus; Lechner, Klaus; Jaeger, Ulrich

    2016-09-01

    We retrospectively studied the prevalence of C1 esterase inhibitor (C1 INH) deficiency in 131 patients with various lymphomas. We determined C1 INH activity, C1 INH antigen, and C4 concentration at diagnosis and after chemotherapy. In follicular lymphoma (FL), diffuse large B-cell lymphoma (DLBCL) and chronic lymphocytic leukemia (CLL) consecutive patients were studied. In these entities, the prevalence of C1 INH deficiency was 10.2% in DLBCL, 4.1% in CLL, and 0% in FL and Hodgkin lymphoma. In indolent lymphomas, we identified only single cases of C1 INH deficiency, predominantly in splenic marginal zone lymphomas (SMZL) (four cases). Only three patients were symptomatic while the majority (11 cases) was asymptomatic. In DLBCL patients who were successfully treated with chemotherapy, complete normalization of C1 INH activity and C4 was observed. In contrast, C1 INH deficiency remained in SMZL patients after splenectomy. We conclude that C1 INH deficiency in lymphomas is frequently asymptomatic and responsive to immunochemotherapy.

  13. Treatment of hereditary angioedema with plasma-derived C1 inhibitor

    PubMed Central

    Prematta, Michael J; Prematta, Tracy; Craig, Timothy J

    2008-01-01

    Background: Plasma-derived C1 inhibitor (C1-INH) concentrate is a treatment option for acute hereditary angioedema (HAE) attacks and is considered the standard-of-care in many countries, although it is not yet available in the United States. Studies are still being conducted to establish its safety and efficacy as required by the FDA. Objective: To review the medical literature to determine if C1-INH concentrate is a safe and effective treatment for acute HAE attacks. Methods: The following keywords were searched in PubMed and OVID: C1 esterase inhibitor, C1-inhibitor, C1 inhibitor, and hereditary angioedema treatment. English-language articles were searched from 1966 to the present to look for studies demonstrating the efficacy and the safety of C1-INH concentrate. Results: The English-language literature search revealed several studies showing significantly improved relief of HAE symptoms with the administration of C1-INH concentrate – many studies demonstrated some improvement of symptoms within 30 minutes. Side effects have been similar to placebo, and no proven cases of viral transmission have occurred in over 20 years. Conclusion: C1-INH concentrate appears to be a very safe and effective treatment option for HAE. PMID:19209279

  14. Contact system activation in patients with HAE and normal C1 inhibitor function.

    PubMed

    Ghannam, Arije; Defendi, Federica; Charignon, Delphine; Csopaki, Françoise; Favier, Bertrand; Habib, Mohammed; Cichon, Sven; Drouet, Christian

    2013-11-01

    In addition to hereditary angioedema (HAE) with C1 inhibitor (C1INH) deficiency, a type of HAE with dominant inheritance and normal C1INH function (HAE with normal C1INH) has been described. This relates to contact phase activation with exaggerated kinin formation, and mutations in the coagulation factor XII gene have been identified in some affected families, but the cause of the disease has remained elusive in a majority of families. Several triggering factors are responsible for developing kinin forming system, with participation of endothelium and mast cell component. Angioedema conditions meet the accumulation of kinins with failed kinin catabolism. PMID:24176216

  15. Fundamentals of Construction. Introduction to Construction Series. Instructor Edition.

    ERIC Educational Resources Information Center

    Oklahoma State Dept. of Vocational and Technical Education, Stillwater. Curriculum and Instructional Materials Center.

    This competency-based curriculum guide begins the Introduction to Construction series. The series is designed with the flexible training requirements of open shop contractors, preapprenticeship programs, multicraft high school programs, technology education programs, and cooperative education programs in mind. This guide contains 3 sections and 15…

  16. The thrombogenicity of C1 esterase inhibitor (human): review of the evidence.

    PubMed

    Crowther, Mark; Bauer, Kenneth A; Kaplan, Allen P

    2014-01-01

    Thromboembolic events associated with human plasma-derived C1 esterase inhibitor (C1-INH) use in patients with hereditary angioedema (HAE) have been reported in the U.S. Food and Drug Administration (FDA) Adverse Event Reporting System database. The purpose of this article is to review and assess the strength of available evidence regarding the thrombogenicity of human plasma-derived C1-INH. A PubMed search was conducted of English language articles from January 1990 to December 2013 reporting the thrombogenicity of C1-INH. Original research articles were selected if the following criteria were met: (1) C1-INH was the focus of the study and (2) the authors addressed the pro- or antithrombotic potential of C1-INH. Additional articles on the clinical use of C1-INH in disease states other than HAE were obtained using reference lists of selected articles. Pivotal studies and prescribing information for C1-INH products were also reviewed. Limited animal and clinical data suggest that C1-INH, particularly at high doses of up to 500 U/kg (compared with the U.S. FDA-approved 20-U/kg dose), may be prothrombotic. In contrast, C1-INH has been used in some patients with myocardial infarction, ischemic stroke, sepsis, and capillary leak syndrome at off-label supratherapeutic doses (up to 100 U/kg) without evidence of a thrombogenic effect. Based on our review, thromboembolic events reported with C1-INH use are rare and patients with HAE who experienced such events often have underlying thromboembolic risk factors.

  17. Functional C1q is present in the skin mucus of Siberian sturgeon (Acipenser baerii).

    PubMed

    Fan, Chunxin; Wang, Jian; Zhang, Xuguang; Song, Jiakun

    2015-01-01

    The skin mucus of fish acts as the first line of self-protection against pathogens in the aquatic environment and comprises a number of innate immune components. However, the presence of the critical classical complement component C1q, which links the innate and adaptive immune systems of mammalians, has not been explored in a primitive actinopterygian fish. In this study, we report that C1q is present in the skin mucus of the Siberian sturgeon (Acipenser baerii). The skin mucus was able to inhibit the growth of Escherichia coli. The bacteriostatic activity of the skin mucus was reduced by heating and by pre-incubation with EDTA or mouse anti-human C1q antibody. We also detected C1q protein in skin mucus using the western blot procedure and isolated a cDNA that encodes the Siberian sturgeon C1qC, which had 44.7-51.4% identity with C1qCs in teleosts and tetrapods. A phylogenetic analysis revealed that Siberian sturgeon C1qC lies at the root of the actinopterygian branch and is separate from the tetrapod branch. The C1qC transcript was expressed in many tissues as well as in skin. Our data indicate that C1q is present in the skin mucus of the Siberian sturgeon to protect against water-borne bacteria, and the C1qC found in the sturgeon may represent the primitive form of teleost and tetrapod C1qCs.

  18. The C1q Family of Proteins: Insights into the Emerging Non-Traditional Functions

    PubMed Central

    Ghebrehiwet, Berhane; Hosszu, Kinga K.; Valentino, Alisa; Peerschke, Ellinor I. B.

    2012-01-01

    Research conducted over the past 20 years have helped us unravel not only the hidden structural and functional subtleties of human C1q, but also has catapulted the molecule from a mere recognition unit of the classical pathway to a well-recognized molecular sensor of damage-modified self or non-self antigens. Thus, C1q is involved in a rapidly expanding list of pathological disorders – including autoimmunity, trophoblast migration, preeclampsia, and cancer. The results of two recent reports are provided to underscore the critical role C1q plays in health and disease. First is the observation by Singh et al. (2011) showing that pregnant C1q−/− mice recapitulate the key features of human preeclampsia that correlate with increased fetal death. Treatment of the C1q−/− mice with pravastatin restored trophoblast invasiveness, placental blood flow, and angiogenic balance and, thus, prevented the onset of preeclampsia. Second is the report by Hong et al. (2009) which showed that C1q can induce apoptosis of prostate cancer cells by activating the tumor suppressor molecule WW-domain containing oxydoreductase (WWOX or WOX1) and destabilizing cell adhesion. Downregulation of C1q on the other hand, enhanced prostate hyperplasia and cancer formation due to failure of WOX1 activation. C1q belongs to a family of structurally and functionally related TNF-α-like family of proteins that may have arisen from a common ancestral gene. Therefore C1q not only shares the diverse functions with the tumor necrosis factor family of proteins, but also explains why C1q has retained some of its ancestral “cytokine-like” activities. This review is intended to highlight some of the structural and functional aspects of C1q by underscoring the growing list of its non-traditional functions. PMID:22536204

  19. Construction aggregates

    USGS Publications Warehouse

    Tepordei, V.V.

    1995-01-01

    Part of the 1994 Industrial Minerals Review. The production, consumption, and applications of construction aggregates are reviewed. In 1994, the production of construction aggregates, which includes crushed stone and construction sand and gravel combined, increased 7.7 percent to 2.14 Gt compared with the previous year. These record production levels are mostly a result of funding for highway construction work provided by the Intermodal Surface Transportation Efficiency Act of 1991. Demand is expected to increase for construction aggregates in 1995.

  20. TPX tokamak construction management

    SciTech Connect

    Knutson, D.; Kungl, D.; Seidel, P.; Halfast, C.

    1995-12-31

    A construction management contract normally involves the acquisition of a construction management firm to assist in the design, planning, budget conformance, and coordination of the construction effort. In addition the construction management firm acts as an agent in the awarding of lower tier contracts. The TPX Tokamak Construction Management (TCM) approach differs in that the construction management firm is also directly responsible for the assembly and installation of the tokamak including the design and fabrication of all tooling required for assembly. The Systems Integration Support (SIS) contractor is responsible for the architect-engineering design of ancillary systems, such as heating and cooling, buildings, modifications and site improvements, and a variety of electrical requirements, including switchyards and >4kV power distribution. The TCM will be responsible for the procurement of materials and the installation of the ancillary systems, which can either be performed directly by the TCM or subcontracted to a lower tier subcontractor. Assurance that the TPX tokamak is properly assembled and ready for operation when turned over to the operations team is the primary focus of the construction management effort. To accomplish this a disciplined constructability program will be instituted. The constructability effort will involve the effective and timely integration of construction expertise into the planning, component design, and field operations. Although individual component design groups will provide liaison during the machine assembly operations, the construction management team is responsible for assembly.