46 CFR 112.30-10 - Restoration of normal source potential.

Code of Federal Regulations, 2012 CFR

2012-10-01

... Section 112.30-10 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatically Connected Storage Battery as the Sole Emergency Power Source § 112.30-10 Restoration of normal source potential. When the potential...

46 CFR 112.35-3 - Normal source for emergency loads.

Code of Federal Regulations, 2011 CFR

2011-10-01

....35-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Manually Controlled Emergency Systems Having a Storage Battery or a Diesel Engine or Gas Turbine Driven Generator as the Sole Emergency Power Source § 112.35-3 Normal source...

46 CFR 112.35-3 - Normal source for emergency loads.

Code of Federal Regulations, 2014 CFR

2014-10-01

....35-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Manually Controlled Emergency Systems Having a Storage Battery or a Diesel Engine or Gas Turbine Driven Generator as the Sole Emergency Power Source § 112.35-3 Normal source...

46 CFR 112.35-3 - Normal source for emergency loads.

Code of Federal Regulations, 2012 CFR

2012-10-01

....35-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Manually Controlled Emergency Systems Having a Storage Battery or a Diesel Engine or Gas Turbine Driven Generator as the Sole Emergency Power Source § 112.35-3 Normal source...

46 CFR 112.35-3 - Normal source for emergency loads.

Code of Federal Regulations, 2010 CFR

2010-10-01

....35-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Manually Controlled Emergency Systems Having a Storage Battery or a Diesel Engine or Gas Turbine Driven Generator as the Sole Emergency Power Source § 112.35-3 Normal source...

46 CFR 112.35-3 - Normal source for emergency loads.

Code of Federal Regulations, 2013 CFR

2013-10-01

....35-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Manually Controlled Emergency Systems Having a Storage Battery or a Diesel Engine or Gas Turbine Driven Generator as the Sole Emergency Power Source § 112.35-3 Normal source...

46 CFR 112.25-1 - General.

Code of Federal Regulations, 2013 CFR

2013-10-01

..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatic Starting Diesel Engine or Gas Turbine Driven Emergency Power Source as the Sole Emergency Power Source § 112.25-1 General. This subpart contains requirements applicable to...

46 CFR 112.25-1 - General.

Code of Federal Regulations, 2014 CFR

2014-10-01

..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatic Starting Diesel Engine or Gas Turbine Driven Emergency Power Source as the Sole Emergency Power Source § 112.25-1 General. This subpart contains requirements applicable to...

46 CFR 112.25-1 - General.

Code of Federal Regulations, 2010 CFR

2010-10-01

..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatic Starting Diesel Engine or Gas Turbine Driven Emergency Power Source as the Sole Emergency Power Source § 112.25-1 General. This subpart contains requirements applicable to...

46 CFR 112.25-1 - General.

Code of Federal Regulations, 2011 CFR

2011-10-01

..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatic Starting Diesel Engine or Gas Turbine Driven Emergency Power Source as the Sole Emergency Power Source § 112.25-1 General. This subpart contains requirements applicable to...

46 CFR 112.25-1 - General.

Code of Federal Regulations, 2012 CFR

2012-10-01

..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatic Starting Diesel Engine or Gas Turbine Driven Emergency Power Source as the Sole Emergency Power Source § 112.25-1 General. This subpart contains requirements applicable to...

46 CFR 112.25-10 - Transfer of emergency loads.

Code of Federal Regulations, 2013 CFR

2013-10-01

... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatic Starting Diesel Engine or Gas Turbine Driven Emergency Power Source as the Sole Emergency Power Source § 112.25-10 Transfer of emergency loads. (a) When...

46 CFR 112.25-10 - Transfer of emergency loads.

Code of Federal Regulations, 2014 CFR

2014-10-01

... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatic Starting Diesel Engine or Gas Turbine Driven Emergency Power Source as the Sole Emergency Power Source § 112.25-10 Transfer of emergency loads. (a) When...

46 CFR 112.25-10 - Transfer of emergency loads.

Code of Federal Regulations, 2012 CFR

2012-10-01

... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatic Starting Diesel Engine or Gas Turbine Driven Emergency Power Source as the Sole Emergency Power Source § 112.25-10 Transfer of emergency loads. (a) When...

46 CFR 112.25-10 - Transfer of emergency loads.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatic Starting Diesel Engine or Gas Turbine Driven Emergency Power Source as the Sole Emergency Power Source § 112.25-10 Transfer of emergency loads. (a) When...

46 CFR 112.25-10 - Transfer of emergency loads.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatic Starting Diesel Engine or Gas Turbine Driven Emergency Power Source as the Sole Emergency Power Source § 112.25-10 Transfer of emergency loads. (a) When...

46 CFR 112.30-1 - General.

Code of Federal Regulations, 2014 CFR

2014-10-01

..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatically Connected Storage Battery as the Sole Emergency Power Source § 112.30-1 General. This subpart contains requirements applicable to emergency power installations having...

46 CFR 112.30-1 - General.

Code of Federal Regulations, 2012 CFR

2012-10-01

..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatically Connected Storage Battery as the Sole Emergency Power Source § 112.30-1 General. This subpart contains requirements applicable to emergency power installations having...

46 CFR 112.30-1 - General.

Code of Federal Regulations, 2011 CFR

2011-10-01

..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatically Connected Storage Battery as the Sole Emergency Power Source § 112.30-1 General. This subpart contains requirements applicable to emergency power installations having...

46 CFR 112.30-1 - General.

Code of Federal Regulations, 2010 CFR

2010-10-01

..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatically Connected Storage Battery as the Sole Emergency Power Source § 112.30-1 General. This subpart contains requirements applicable to emergency power installations having...

46 CFR 112.30-1 - General.

Code of Federal Regulations, 2013 CFR

2013-10-01

..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatically Connected Storage Battery as the Sole Emergency Power Source § 112.30-1 General. This subpart contains requirements applicable to emergency power installations having...

46 CFR 112.35-1 - General.

Code of Federal Regulations, 2014 CFR

2014-10-01

..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS... Generator as the Sole Emergency Power Source § 112.35-1 General. This subpart contains requirements applicable to emergency power installations having a manually controlled storage battery, diesel engine, or...

46 CFR 112.35-1 - General.

Code of Federal Regulations, 2012 CFR

2012-10-01

..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS... Generator as the Sole Emergency Power Source § 112.35-1 General. This subpart contains requirements applicable to emergency power installations having a manually controlled storage battery, diesel engine, or...

46 CFR 112.35-1 - General.

Code of Federal Regulations, 2013 CFR

2013-10-01

..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS... Generator as the Sole Emergency Power Source § 112.35-1 General. This subpart contains requirements applicable to emergency power installations having a manually controlled storage battery, diesel engine, or...

77 FR 12930 - Federal Acquisition Regulation: Socioeconomic Program Parity

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-02

... on May 6, 2011, reinstating the Rule of Two. C. Sole Source Dollar Thresholds Vary Among the... all socioeconomic programs had the same sole source dollar threshold. Response: The sole source dollar... business socioeconomic contracting program to utilize. D. Sole Source Authority Under the SDVOSB Program...

21 CFR 520.2089 - Roxarsone liquid.

Code of Federal Regulations, 2010 CFR

2010-04-01

... and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) ANIMAL... teaspoon (5 milliliters) to each gallon of drinking water (0.002 percent roxarsone). (2) Indications for... for human consumption. Withdraw 5 days before slaughter. Use as sole source of organic arsenic...

21 CFR 520.1807 - Piperazine.

Code of Federal Regulations, 2010 CFR

2010-04-01

... Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) ANIMAL DRUGS... in drinking water or feed. Use as sole source of drinking water. Prepare fresh solution daily. Use as... for human consumption. Consult your veterinarian for assistance in the diagnosis, treatment, and...

Soy protein isolate protects against ethanol mediated tumor progression in diethylnitrosamine treated male mice

USDA-ARS?s Scientific Manuscript database

In this study, DEN-treated male mice were assigned to 3 groups: a 35% high fat ethanol liquid diet (EtOH) with casein as the protein source, the same EtOH liquid diet with soy protein isolate as the sole protein source (EtOH/soy) and a chow group. EtOH feeding continued for 16 wks. As expected, E...

46 CFR 112.30-5 - Transfer of emergency loads.

Code of Federal Regulations, 2014 CFR

2014-10-01

... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatically Connected Storage Battery as the Sole Emergency Power Source § 112.30-5 Transfer of emergency loads. If there is a reduction of potential of the...

46 CFR 112.35-5 - Manually started emergency systems.

Code of Federal Regulations, 2011 CFR

2011-10-01

....35-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Manually Controlled Emergency Systems Having a Storage Battery or a Diesel Engine or Gas Turbine Driven Generator as the Sole Emergency Power Source § 112.35-5 Manually...

46 CFR 112.35-5 - Manually started emergency systems.

Code of Federal Regulations, 2013 CFR

2013-10-01

....35-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Manually Controlled Emergency Systems Having a Storage Battery or a Diesel Engine or Gas Turbine Driven Generator as the Sole Emergency Power Source § 112.35-5 Manually...

46 CFR 112.30-5 - Transfer of emergency loads.

Code of Federal Regulations, 2012 CFR

2012-10-01

... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatically Connected Storage Battery as the Sole Emergency Power Source § 112.30-5 Transfer of emergency loads. If there is a reduction of potential of the...

46 CFR 112.35-5 - Manually started emergency systems.

Code of Federal Regulations, 2014 CFR

2014-10-01

....35-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Manually Controlled Emergency Systems Having a Storage Battery or a Diesel Engine or Gas Turbine Driven Generator as the Sole Emergency Power Source § 112.35-5 Manually...

46 CFR 112.35-5 - Manually started emergency systems.

Code of Federal Regulations, 2012 CFR

2012-10-01

....35-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Manually Controlled Emergency Systems Having a Storage Battery or a Diesel Engine or Gas Turbine Driven Generator as the Sole Emergency Power Source § 112.35-5 Manually...

46 CFR 112.30-5 - Transfer of emergency loads.

Code of Federal Regulations, 2013 CFR

2013-10-01

... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatically Connected Storage Battery as the Sole Emergency Power Source § 112.30-5 Transfer of emergency loads. If there is a reduction of potential of the...

46 CFR 112.30-5 - Transfer of emergency loads.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatically Connected Storage Battery as the Sole Emergency Power Source § 112.30-5 Transfer of emergency loads. If there is a reduction of potential of the...

46 CFR 112.35-5 - Manually started emergency systems.

Code of Federal Regulations, 2010 CFR

2010-10-01

....35-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Manually Controlled Emergency Systems Having a Storage Battery or a Diesel Engine or Gas Turbine Driven Generator as the Sole Emergency Power Source § 112.35-5 Manually...

46 CFR 112.30-5 - Transfer of emergency loads.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatically Connected Storage Battery as the Sole Emergency Power Source § 112.30-5 Transfer of emergency loads. If there is a reduction of potential of the...

40 CFR 149.111 - Funding to redesigned projects.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 23 2011-07-01 2011-07-01 false Funding to redesigned projects. 149.111 Section 149.111 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER... Designated Sole Source Aquifer in the San Antonio, Texas Area § 149.111 Funding to redesigned projects. After...

EPA Region 1 Sole Source Aquifers

EPA Pesticide Factsheets

This coverage contains boundaries of EPA-approved sole source aquifers. Sole source aquifers are defined as an aquifer designated as the sole or principal source of drinking water for a given aquifer service area; that is, an aquifer which is needed to supply 50% or more of the drinking water for the area and for which there are no reasonable alternative sources should the aquifer become contaminated.The aquifers were defined by a EPA hydrogeologist. Aquifer boundaries were then drafted by EPA onto 1:24000 USGS quadrangles. For the coastal sole source aquifers the shoreline as it appeared on the quadrangle was used as a boundary. Delineated boundaries were then digitized into ARC/INFO.

40 CFR 149.3 - Critical Aquifer Protection Areas.

Code of Federal Regulations, 2010 CFR

2010-07-01

... ground-water quality protection plan was approved, under section 208 of the Clean Water Act, prior to that date; or (b) All or part of a major recharge area of a sole or principal source aquifer....3 Section 149.3 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS...

40 CFR 149.3 - Critical Aquifer Protection Areas.

Code of Federal Regulations, 2011 CFR

2011-07-01

... ground-water quality protection plan was approved, under section 208 of the Clean Water Act, prior to that date; or (b) All or part of a major recharge area of a sole or principal source aquifer....3 Section 149.3 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS...

Sole Source Aquifers for NY and NJ

EPA Pesticide Factsheets

This layer is the designated sole source aquifers of New York and New Jersey. A Sole Source Aquifer, is an aquifer that supplies 50% or more of the drinking water for a given area where there are no reasonably available alternative sources should the aquifer become contaminated.

21 CFR 558.62 - Arsanilic acid.

Code of Federal Regulations, 2011 CFR

2011-04-01

... efficiency; improving pigmentation Withdraw 5 days before slaughter; as sole source of organic arsenic 015565... pigmentation. As erythromycin thiocyanate; withdraw 5 days before slaughter; as sole source of organic arsenic... pigmentation As erythromycin thiocyanate; withdraw 5 days before slaughter; as sole source of organic arsenic...

Microbial utilization of electrically reduced neutral red as the sole electron donor for growth and metabolite production

DOE Office of Scientific and Technical Information (OSTI.GOV)

Park, D.H.; Laivenieks, M.; Guettler, M.V.

1999-07-01

Electrically reduced neutral red (NR) served as the sole source of reducing power for growth and metabolism of pure and mixed cultures of H[sub 2]-consuming bacteria in a novel electrochemical bioreactor system. NR was continuously reduced by the cathodic potential ([minus]1.5 V) generated from an electric current (0.3 to 1.0 mA), and it was subsequently oxidized by Actinobacillus succinogenes or by mixed methanogenic cultures. The A. succinogenes mutant strain FZ-6 did not grow on fumarate alone unless electrically reduced NR or hydrogen was present as the electron donor for succinate production. The mutant strain, unlike the wild type, lacked pyruvatemore » formate lyase and formate dehydrogenase. Electrically reduced NR also replaced hydrogen as the sole electron donor source for growth and production of methane from CO[sub 2]. These results show that both pure and mixed cultures can function as electrochemical devices when electrically generated reducing power can be used to drive metabolism. The potential utility of utilizing electrical reducing power in enhancing industrial fermentations or biotransformation processes is discussed.« less

Biodegradation of Organofluorine Compounds

DTIC Science & Technology

2016-02-01

BIODEGRADATION OF ORGANOFLUORINE COMPOUNDS ECBC-TR-1347 Melissa M. Dixon Steve P. Harvey RESEARCH AND...2011 4. TITLE AND SUBTITLE Biodegradation of Organofluorine Compounds 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR...compounds as sole carbon sources. This work will be continued in future studies. 15. SUBJECT TERMS Organofluorine Biodegradation Defluorination

21 CFR 520.2088 - Roxarsone tablets.

Code of Federal Regulations, 2011 CFR

2011-04-01

... period. Withdraw 5 days before slaughter. Use as sole source of organic arsenic. (ii) Growing chickens—(a.... Withdraw 5 days before slaughter. Use as sole source of organic arsenic. (b)(1) Specifications. Each tablet... slaughter. Use as sole source of organic arsenic. (ii) [Reserved] (c)(1) Specifications. Each tablet...

Breastfeeding and Mental and Motor Development at 5 ½ Years

PubMed Central

Clark, Katy M.; Castillo, Marcela; Calatroni, Agustin; Walter, Tomas; Cayazzo, Marisol; Pino, Paulina; Lozoff, Betsy

2006-01-01

Objective Breastfeeding is associated with better child development outcomes, but uncertainty remains primarily due to the close relationship between breastfeeding and socioeconomic status. This study assesses the issue in a low socioeconomic status sample where breastfeeding was close to universal. Methods 784 Chilean children were followed longitudinally from infancy. All but 4 were initially breastfed, 40% nursed beyond 12 months, and infant growth was normal. Child development was assessed at 5 ½ years by a cognitive, language, and motor test battery. The duration of breastfeeding as the sole milk source was analyzed as a continuous variable, adjusting for a comprehensive set of background factors. Results The relationship between breastfeeding and most 5 ½ -year developmental outcomes was non-linear, with poorer outcome for periods of breastfeeding as the sole milk source for < 2 months or > 8 months – statistically significant for language, motor, and one comprehensive cognitive test, with a suggestive trend for IQ. Conclusions The observed non-linear relationships showed that breastfeeding as the sole milk source for < 2 months or > 8 months, compared to 2–8 months, was associated with poorer development in this sample. The latter finding requires replication in other samples where long breastfeeding is common and socioeconomic status is relatively homogeneous. PMID:16530141

75 FR 38687 - Federal Acquisition Regulation; FAR Case 2008-023, Clarification of Criteria for Sole Source...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-02

... a sole source SDVOSB concern acquisition. The final rule contains language that more closely mirrors...-AL29 Federal Acquisition Regulation; FAR Case 2008-023, Clarification of Criteria for Sole Source...: Final rule. SUMMARY: The Civilian Agency Acquisition Council and the Defense Acquisition Regulations...

INTEGRATED HUMAN AND ECOLOIGCAL RISK ASSESSMENT: A CASE STUDY OF ULTRAVIOLET RADIATION EFFECTS ON AMPHIBIANS, CORAL, HUMANS, AND OCEANIC PRIMARY PRODUCTIVITY

EPA Science Inventory

Ultraviolet radiation (UVR) is a naturally occurring stressor to most forms of life. The sole relevant source of this stressor is the sun. The Earth's stratospheric ozone layer reduces the amount of UVR that reaches the Earth's surface. The potential for continued depletion of th...

78 FR 54652 - Sole Source Cooperative Agreement Award to the Association for State and Territorial Health...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-05

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Sole Source Cooperative Agreement Award to the Association...: Notification of a Sole Source Cooperative Agreement Award to the Association for State and Territorial Health... management systems. One of the overarching goals of both the National Health Security Strategy (2009) and the...

78 FR 54651 - Sole Source Cooperative Agreement Award to the National Association of County and City Health...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-05

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Sole Source Cooperative Agreement Award to the National...: Notification of a Sole Source Cooperative Agreement Award to the National Association of County and City Health... resilience, and strengthen health care, public health, and emergency management systems. One of the...

Defense Logistics Agency Did Not Obtain Fair and Reasonable Prices From Meggitt Aircraft Braking Systems for Sole-Source Commercial Spare Parts (REDACTED)

DTIC Science & Technology

2015-05-08

Defense Logistics Agency Did Not Obtain Fair and Reasonable Prices From Meggitt Aircraft Braking Systems for Sole-Source Commercial Spare Parts...Defense Logistics Agency Did Not Obtain Fair and Reasonable Prices From Meggitt Aircraft Braking Systems for Sole-Source Commercial Spare Parts...D000AH-0180.000) │ i Results in Brief Defense Logistics Agency Did Not Obtain Fair and Reasonable Prices From Meggitt Aircraft Braking Systems for

21 CFR 520.2240b - Sulfaethoxypyridazine tablets.

Code of Federal Regulations, 2010 CFR

2010-04-01

...; do not treat within 16 days of slaughter; as sole source of sulfonamide; milk that has been taken... sole source of sulfonamide; not for use in lactating dairy cows; Federal law restricts this drug to use...

21 CFR 520.2087 - Roxarsone soluble powder.

Code of Federal Regulations, 2010 CFR

2010-04-01

... throughout growing period. Withdraw 5 days before slaughter. Use as sole source of organic arsenic. (2) Swine.... Use as sole source of organic arsenic. [46 FR 41039, Aug. 14, 1981, as amended at 55 FR 8460, Mar. 8...

78 FR 19261 - Safe Drinking Water Act Sole Source Aquifer Program; Designation of Bainbridge Island, Washington...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-29

... aquifer (U.S. EPA, 1987, Sole Source Aquifer Designation Decision Process, Petition Review Guidance... the petition; U.S. Geological Survey, 2011, Conceptual Model and Numerical Simulation of the...

21 CFR 520.2087 - Roxarsone soluble powder.

Code of Federal Regulations, 2011 CFR

2011-04-01

... throughout growing period. Withdraw 5 days before slaughter. Use as sole source of organic arsenic. (2) Swine.... Use as sole source of organic arsenic. [46 FR 41039, Aug. 14, 1981, as amended at 55 FR 8460, Mar. 8...

Light intensity and quality from sole-source light-emitting diodes impact growth, morphology, and nutrient content of Brassica microgreens

USDA-ARS?s Scientific Manuscript database

Multi-layer vertical production systems using sole-source (SS) lighting can be used for microgreen production; however, traditional SS lighting can consume large amounts of electrical energy. Light-emitting diodes (LEDs) offer many advantages over conventional light sources including: high photoelec...

Nitrogen transformation under different dissolved oxygen levels by the anoxygenic phototrophic bacterium Marichromatium gracile.

PubMed

Hong, Xuan; Chen, Zhongwei; Zhao, Chungui; Yang, Suping

2017-06-01

Marichromatium gracile: YL28 (M. gracile YL28) is an anoxygenic phototrophic bacterial strain that utilizes ammonia, nitrate, or nitrite as its sole nitrogen source during growth. In this study, we investigated the removal and transformation of ammonium, nitrate, and nitrite by M. gracile YL28 grown in a combinatorial culture system of sodium acetate-ammonium, sodium acetate-nitrate and sodium acetate-nitrite in response to different initial dissolved oxygen (DO) levels. In the sodium acetate-ammonium system under aerobic conditions (initial DO = 7.20-7.25 mg/L), we detected a continuous accumulation of nitrate and nitrite. However, under semi-anaerobic conditions (initial DO = 4.08-4.26 mg/L), we observed a temporary accumulation of nitrate and nitrite. Interestingly, under anaerobic conditions (initial DO = 0.36-0.67 mg/L), there was little accumulation of nitrate and nitrite, but an increase in nitrous oxide production. In the sodium acetate-nitrite system, nitrite levels declined slightly under aerobic conditions, and nitrite was completely removed under semi-anaerobic and anaerobic conditions. In addition, M. gracile YL28 was able to grow using nitrite as the sole nitrogen source in situations when nitrogen gas produced by denitrification was eliminated. Taken together, the data indicate that M. gracile YL28 performs simultaneous heterotrophic nitrification and denitrification at low-DO levels and uses nitrite as the sole nitrogen source for growth. Our study is the first to demonstrate that anoxygenic phototrophic bacteria perform heterotrophic ammonia-oxidization and denitrification under anaerobic conditions.

Formation of Hydride-Meisenheimer Complexes of Picric Acid (2,4,6-Trinitrophenol) and 2,4-Dinitrophenol during Mineralization of Picric Acid by Nocardioides sp. Strain CB 22-2

PubMed Central

Behrend, Christian; Heesche-Wagner, Kerstin

1999-01-01

There are only a few examples of microbial conversion of picric acid (2,4,6-trinitrophenol). None of the organisms that have been described previously is able to use this compound as a sole source of carbon, nitrogen, and energy at high rates. In this study we isolated and characterized a strain, strain CB 22-2, that was able to use picric acid as a sole source of carbon and energy at concentrations up to 40 mM and at rates of 1.6 mmol · h−1 · g (dry weight) of cells−1 in continuous cultures and 920 μmol · h−1 · g (dry weight) of cells−1 in flasks. In addition, this strain was able to use picric acid as a sole source of nitrogen at comparable rates in a nitrogen-free medium. Biochemical characterization and 16S ribosomal DNA analysis revealed that strain CB 22-2 is a Nocardioides sp. strain. High-pressure liquid chromatography and UV-visible light data, the low residual chemical oxygen demand, and the stoichiometric release of 2.9 ± 0.1 mol of nitrite per mol of picric acid provided strong evidence that complete mineralization of picric acid occurred. During transformation, the metabolites detected in the culture supernatant were the [H−]-Meisenheimer complexes of picric acid and 2,4-dinitrophenol (H−-DNP), as well as 2,4-dinitrophenol. Experiments performed with crude extracts revealed that H−-DNP formation indeed is a physiologically relevant step in picric acid metabolism. PMID:10103224

Electricity generation from synthesis gas by microbial processes: CO fermentation and microbial fuel cell technology.

PubMed

Kim, Daehee; Chang, In Seop

2009-10-01

A microbiological process was established to harvest electricity from the carbon monoxide (CO). A CO fermenter was enriched with CO as the sole carbon source. The DGGE/DNA sequencing results showed that Acetobacterium spp. were enriched from the anaerobic digester fluid. After the fermenter was operated under continuous mode, the products were then continuously fed to the microbial fuel cell (MFC) to generate electricity. Even though the conversion yield was quite low, this study proved that synthesis gas (syn-gas) can be converted to electricity with the aid of microbes that do not possess the drawbacks of metal catalysts of conventional methods.

Biodegradation of Nitriles in Shale Oil

PubMed Central

Aislabie, Jackie; Atlas, Ronald M.

1988-01-01

Enrichment cultures were obtained, after prolonged incubation on a shale oil as the sole source of nitrogen, that selectively degraded nitriles. Capillary gas chromatographic analyses showed that the mixed microbial populations in the enrichments degraded the homologous series of aliphatic nitriles but not the aliphatic hydrocarbons, aromatic hydrocarbons, or heterocyclic-nitrogen compounds found in this oil. Time course studies showed that lighter nitriles were removed more rapidly than higher-molecular-weight nitriles. A Pseudomonas fluorescens strain isolated from an enrichment, which was able to completely utilize the individual nitriles undecyl cyanide and undecanenitrile as sole sources of carbon and nitrogen, was unable to attack stearonitrile when provided alone as the growth substrate. A P. aeruginosa strain, also isolated from one of the enrichments, used nitriles but not aliphatic or aromatic hydrocarbons when the oil was used as a sole nitrogen source. However, when the shale oil was used as the sole source of carbon, aliphatic hydrocarbons in addition to nitriles were degraded but aromatic hydrocarbons were still not attacked by this P. aeruginosa strain. PMID:16347731

Alternate Sources for Propellant Ingredients.

DTIC Science & Technology

1976-07-07

0dJ variety of reasons; (3) sole source; (4) medical/ OSHA /EPA problems; (5) dependent on foreign Imports; and (6) specification problems. •’. .’ . . I...problems exist for a variety of reasons; (3) sole sourc:e; (4) medical/ OSHA /EPA problems; (5) dependent on foreign imports; and (6) specification problems...regulations of OSHA or EPA affect pro- duction or use of the product; 5. Plant capacity - when demand increases faster that; predictions; 6. Supply

Number versus Extent in Newborns' Spontaneous Preference for Collections of Dots

ERIC Educational Resources Information Center

Turati, Chiara; Gava, Lucia; Valenza, Eloisa; Ghirardi, Valentina

2013-01-01

This study investigated processing of number and extent in newborns. Using visual preference, we showed that newborns discriminated between small sets of dot collections relying solely on implicit numerical information when non-numerical continuous variables were strictly controlled (Experiment 1), and solely on continuous information when…

77 FR 35624 - Federal Acquisition Regulation; Justification and Approval of Sole-Source 8(a) Contracts: Correction

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-14

... 2] RIN 9000-AL55 Federal Acquisition Regulation; Justification and Approval of Sole-Source 8(a... removes language indicating that the applicable section of the National Defense Authorization Act for...: June 8, 2012. Laura Auletta, Director, Office of Governmentwide Acquisition Policy, Office of...

48 CFR 19.1406 - Sole source awards to service-disabled veteran-owned small business concerns.

Code of Federal Regulations, 2013 CFR

2013-10-01

... service-disabled veteran-owned small business concerns. 19.1406 Section 19.1406 Federal Acquisition...-Disabled Veteran-Owned Small Business Procurement Program 19.1406 Sole source awards to service-disabled...-disabled veteran-owned small business concerns; (2) The anticipated award price of the contract, including...

48 CFR 19.1406 - Sole source awards to service-disabled veteran-owned small business concerns.

Code of Federal Regulations, 2014 CFR

2014-10-01

... service-disabled veteran-owned small business concerns. 19.1406 Section 19.1406 Federal Acquisition...-Disabled Veteran-Owned Small Business Procurement Program 19.1406 Sole source awards to service-disabled...-disabled veteran-owned small business concerns; (2) The anticipated award price of the contract, including...

48 CFR 19.1406 - Sole source awards to service-disabled veteran-owned small business concerns.

Code of Federal Regulations, 2010 CFR

2010-10-01

... service-disabled veteran-owned small business concerns. 19.1406 Section 19.1406 Federal Acquisition...-Disabled Veteran-Owned Small Business Procurement Program 19.1406 Sole source awards to service-disabled veteran-owned small business concerns. (a) A contracting officer may award contracts to service-disabled...

48 CFR 18.116 - Service-disabled Veteran-owned Small Business (SDVOSB) sole source awards.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Service-disabled Veteran... Available Acquisition Flexibilities 18.116 Service-disabled Veteran-owned Small Business (SDVOSB) sole source awards. Contracts may be awarded to Service-disabled Veteran-owned Small Business (SDVOSB...

48 CFR 819.7008 - Sole source awards to veteran-owned small business concerns.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Sole source awards to veteran-owned small business concerns. 819.7008 Section 819.7008 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Service-Disabled Veteran-Owned...

48 CFR 19.1406 - Sole source awards to service-disabled veteran-owned small business concerns.

Code of Federal Regulations, 2011 CFR

2011-10-01

... service-disabled veteran-owned small business concerns. 19.1406 Section 19.1406 Federal Acquisition...-Disabled Veteran-Owned Small Business Procurement Program 19.1406 Sole source awards to service-disabled veteran-owned small business concerns. (a) A contracting officer may award contracts to service-disabled...

48 CFR 18.116 - Service-disabled Veteran-owned Small Business (SDVOSB) sole source awards.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 1 2012-10-01 2012-10-01 false Service-disabled Veteran... Available Acquisition Flexibilities 18.116 Service-disabled Veteran-owned Small Business (SDVOSB) sole source awards. Contracts may be awarded to Service-disabled Veteran-owned Small Business (SDVOSB...

48 CFR 18.116 - Service-disabled Veteran-owned Small Business (SDVOSB) sole source awards.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 1 2013-10-01 2013-10-01 false Service-disabled Veteran... Available Acquisition Flexibilities 18.116 Service-disabled Veteran-owned Small Business (SDVOSB) sole source awards. Contracts may be awarded to Service-disabled Veteran-owned Small Business (SDVOSB...

48 CFR 819.7008 - Sole source awards to veteran-owned small business concerns.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Sole source awards to veteran-owned small business concerns. 819.7008 Section 819.7008 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Service-Disabled Veteran-Owned...

48 CFR 18.116 - Service-disabled Veteran-owned Small Business (SDVOSB) sole source awards.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 1 2014-10-01 2014-10-01 false Service-disabled Veteran... Available Acquisition Flexibilities 18.116 Service-disabled Veteran-owned Small Business (SDVOSB) sole source awards. Contracts may be awarded to Service-disabled Veteran-owned Small Business (SDVOSB...

48 CFR 19.1406 - Sole source awards to service-disabled veteran-owned small business concerns.

Code of Federal Regulations, 2012 CFR

2012-10-01

... service-disabled veteran-owned small business concerns. 19.1406 Section 19.1406 Federal Acquisition...-Disabled Veteran-Owned Small Business Procurement Program 19.1406 Sole source awards to service-disabled...-disabled veteran-owned small business concerns; (2) The anticipated award price of the contract, including...

48 CFR 18.116 - Service-disabled Veteran-owned Small Business (SDVOSB) sole source awards.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Service-disabled Veteran... Available Acquisition Flexibilities 18.116 Service-disabled Veteran-owned Small Business (SDVOSB) sole source awards. Contracts may be awarded to Service-disabled Veteran-owned Small Business (SDVOSB...

The association between a journal’s source of revenue and the drug recommendations made in the articles it publishes

PubMed Central

Becker, Annette; Dörter, Fatma; Eckhardt, Kirsten; Viniol, Annika; Baum, Erika; Kochen, Michael M.; Lexchin, Joel; Wegscheider, Karl; Donner-Banzhoff, Norbert

2011-01-01

Background There is evidence to suggest that pharmaceutical companies influence the publication and content of research papers. Most German physicians rely on journals for their continuing medical education. We studied the influence of pharmaceutical advertising on the drug recommendations made in articles published in 11 German journals that focus on continuing medical education. Methods We conducted a cross-sectional study of all of the issues of 11 journals published in 2007. Only journals frequently read by general practitioners were chosen. Issues were screened for pharmaceutical advertisements and recommendations made in the editorial content for a specified selection of drugs. Each journal was rated on a five-point scale according to the strength with which it either recommended or discouraged the use of these drugs. We looked for differences in these ratings between free journals (i.e., those financed entirely by pharmaceutical advertising), journals with mixed sources of revenue and journals financed solely by subscription fees. The journals were also screened for the simultaneous appearance of advertisements and recommendations for the same drug within a certain period, which was adjusted for both journal and class of drug. Results We identified 313 issues containing at least one advertisement for the selected drugs and 412 articles in which drug recommendations were made. Free journals were more likely to recommend the specified drugs than journals with sources of revenue that were mixed or based solely on subscriptions. The simultaneous appearance of advertisements and recommendations for the same drug in the same issue of a journal showed an inconsistent association. Interpretation Free journals almost exclusively recommended the use of the specified drugs, whereas journals financed entirely with subscription fees tended to recommend against the use of the same drugs. Doctors should be aware of this bias in their use of material published in medical journals that focus on continuing medical education. PMID:21398223

The association between a journal's source of revenue and the drug recommendations made in the articles it publishes.

PubMed

Becker, Annette; Dörter, Fatma; Eckhardt, Kirsten; Viniol, Annika; Baum, Erika; Kochen, Michael M; Lexchin, Joel; Wegscheider, Karl; Donner-Banzhoff, Norbert

2011-03-22

There is evidence to suggest that pharmaceutical companies influence the publication and content of research papers. Most German physicians rely on journals for their continuing medical education. We studied the influence of pharmaceutical advertising on the drug recommendations made in articles published in 11 German journals that focus on continuing medical education. We conducted a cross-sectional study of all of the issues of 11 journals published in 2007. Only journals frequently read by general practitioners were chosen. Issues were screened for pharmaceutical advertisements and recommendations made in the editorial content for a specified selection of drugs. Each journal was rated on a five-point scale according to the strength with which it either recommended or discouraged the use of these drugs. We looked for differences in these ratings between free journals (i.e., those financed entirely by pharmaceutical advertising), journals with mixed sources of revenue and journals financed solely by subscription fees. The journals were also screened for the simultaneous appearance of advertisements and recommendations for the same drug within a certain period, which was adjusted for both journal and class of drug. We identified 313 issues containing at least one advertisement for the selected drugs and 412 articles in which drug recommendations were made. Free journals were more likely to recommend the specified drugs than journals with sources of revenue that were mixed or based solely on subscriptions. The simultaneous appearance of advertisements and recommendations for the same drug in the same issue of a journal showed an inconsistent association. Free journals almost exclusively recommended the use of the specified drugs, whereas journals financed entirely with subscription fees tended to recommend against the use of the same drugs. Doctors should be aware of this bias in their use of material published in medical journals that focus on continuing medical education.

40 CFR 80.169 - Liability for violations of the detergent certification program controls and prohibitions.

Code of Federal Regulations, 2011 CFR

2011-07-01

... ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) REGULATION OF FUELS AND FUEL ADDITIVES... violations caused solely by the addition of misadditized ethanol or other PRC to the gasoline. In any case in...(a) caused solely by another party's addition of misadditized ethanol or other PRC to the gasoline...

Molybdenum cofactor (chlorate-resistant) mutants of Klebsiella pneumoniae M5al can use hypoxanthine as the sole nitrogen source.

PubMed Central

Garzón, A; Li, J; Flores, A; Casadesus, J; Stewart, V

1992-01-01

Selection for chlorate resistance yields mol (formerly chl) mutants with defects in molybdenum cofactor synthesis. Complementation and genetic mapping analyses indicated that the Klebsiella pneumoniae mol genes are functionally homologous to those of Escherichia coli and occupy analogous genetic map positions. Hypoxanthine utilization in other organisms requires molybdenum cofactor as a component of xanthine dehydrogenase, and thus most chlorate-resistant mutants cannot use hypoxanthine as a sole source of nitrogen. Surprisingly, the K. pneumoniae mol mutants and the mol+ parent grew equally well with hypoxanthine as the sole nitrogen source, suggesting that K. pneumoniae has a molybdenum cofactor-independent pathway for hypoxanthine utilization. PMID:1400180

Effect of specific amino acids on growth and aflatoxin production by Aspergillus parasiticus and Aspergillus flavus in defined media.

PubMed Central

Payne, G A; Hagler, W M

1983-01-01

Four amino acids were used as sole nitrogen sources or as supplements to ammonium sulfate, and casein and ammonium sulfate were used as sole nitrogen sources to examine their effects on aflatoxin production by Aspergillus parasiticus NRRL 2999 and Aspergillus flavus 3357 grown on synthetic liquid media. In general, when proline, asparagine, casein, and ammonium sulfate were used as sole nitrogen sources, they supported more growth and toxin production than tryptophan or methionine. However, proline stimulated more toxin production per gram of mycelium in stationary cultures than the other nitrogen sources, including the amino acid asparagine, which is generally recognized as supporting good aflatoxin production. The exact responses to individual nitrogen sources were influenced by the species of fungus and whether cultures were stationary or shaken. In shake cultures, but not in stationary cultures, increased growth was generally associated with increased toxin production. PMID:6416168

31 CFR 800.223 - Solely for the purpose of passive investment.

Code of Federal Regulations, 2010 CFR

2010-07-01

... (Continued) OFFICE OF INVESTMENT SECURITY, DEPARTMENT OF THE TREASURY REGULATIONS PERTAINING TO MERGERS, ACQUISITIONS, AND TAKEOVERS BY FOREIGN PERSONS Definitions § 800.223 Solely for the purpose of passive... Board of Directors. The acquisition by Corporation A of a voting interest in Corporation B is not solely...

48 CFR 819.7007 - Sole source awards to service-disabled veteran-owned small business concerns.

Code of Federal Regulations, 2010 CFR

2010-10-01

... service-disabled veteran-owned small business concerns. 819.7007 Section 819.7007 Federal Acquisition...-Disabled Veteran-Owned and Veteran-Owned Small Business Acquisition Program 819.7007 Sole source awards to service-disabled veteran-owned small business concerns. (a) A contracting officer may award contracts to...

48 CFR 819.7007 - Sole source awards to service-disabled veteran-owned small business concerns.

Code of Federal Regulations, 2011 CFR

2011-10-01

... service-disabled veteran-owned small business concerns. 819.7007 Section 819.7007 Federal Acquisition...-Disabled Veteran-Owned and Veteran-Owned Small Business Acquisition Program 819.7007 Sole source awards to service-disabled veteran-owned small business concerns. (a) A contracting officer may award contracts to...

48 CFR 819.7007 - Sole source awards to service-disabled veteran-owned small business concerns.

Code of Federal Regulations, 2014 CFR

2014-10-01

... service-disabled veteran-owned small business concerns. 819.7007 Section 819.7007 Federal Acquisition...-Disabled Veteran-Owned and Veteran-Owned Small Business Acquisition Program 819.7007 Sole source awards to service-disabled veteran-owned small business concerns. (a) A contracting officer may award contracts to...

48 CFR 819.7007 - Sole source awards to service-disabled veteran-owned small business concerns.

Code of Federal Regulations, 2012 CFR

2012-10-01

... service-disabled veteran-owned small business concerns. 819.7007 Section 819.7007 Federal Acquisition...-Disabled Veteran-Owned and Veteran-Owned Small Business Acquisition Program 819.7007 Sole source awards to service-disabled veteran-owned small business concerns. (a) A contracting officer may award contracts to...

48 CFR 819.7007 - Sole source awards to service-disabled veteran-owned small business concerns.

Code of Federal Regulations, 2013 CFR

2013-10-01

... service-disabled veteran-owned small business concerns. 819.7007 Section 819.7007 Federal Acquisition...-Disabled Veteran-Owned and Veteran-Owned Small Business Acquisition Program 819.7007 Sole source awards to service-disabled veteran-owned small business concerns. (a) A contracting officer may award contracts to...

75 FR 53269 - Federal Acquisition Regulation; Tribal Consultation; Justification and Approval of Sole-Source 8...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-31

... Administration (NASA). ACTION: Notice of tribal consultation and outreach meetings and request for comments... owned by Indian Tribes and Alaska Native Corporations, if the sole-source award is to exceed $20 million... Indian Tribes and Alaska Native Corporations. As explained in E.O. 13175, Tribal consultation is a...

Continuous operation of an ultra-low-power microcontroller using glucose as the sole energy source.

PubMed

Lee, Inyoung; Sode, Takashi; Loew, Noya; Tsugawa, Wakako; Lowe, Christopher Robin; Sode, Koji

2017-07-15

An ultimate goal for those engaged in research to develop implantable medical devices is to develop mechatronic implantable artificial organs such as artificial pancreas. Such devices would comprise at least a sensor module, an actuator module, and a controller module. For the development of optimal mechatronic implantable artificial organs, these modules should be self-powered and autonomously operated. In this study, we aimed to develop a microcontroller using the BioCapacitor principle. A direct electron transfer type glucose dehydrogenase was immobilized onto mesoporous carbon, and then deposited on the surface of a miniaturized Au electrode (7mm 2 ) to prepare a miniaturized enzyme anode. The enzyme fuel cell was connected with a 100 μF capacitor and a power boost converter as a charge pump. The voltage of the enzyme fuel cell was increased in a stepwise manner by the charge pump from 330mV to 3.1V, and the generated electricity was charged into a 100μF capacitor. The charge pump circuit was connected to an ultra-low-power microcontroller. Thus prepared BioCapacitor based circuit was able to operate an ultra-low-power microcontroller continuously, by running a program for 17h that turned on an LED every 60s. Our success in operating a microcontroller using glucose as the sole energy source indicated the probability of realizing implantable self-powered autonomously operated artificial organs, such as artificial pancreas. Copyright © 2016 Elsevier B.V. All rights reserved.

Production of FucoPol by Enterobacter A47 using waste tomato paste by-product as sole carbon source.

PubMed

Antunes, Sílvia; Freitas, Filomena; Sevrin, Chantal; Grandfils, Christian; Reis, Maria A M

2017-03-01

Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide. Among the different tested fed-batch strategies, pH-stat, DO-stat and continuous substrate feeding, the highest production (8.77gL -1 ) and overall volumetric productivity (2.92gL -1 d -1 ) were obtained with continuous substrate feeding at a constant flow rate of 11gh -1 . The polymer produced had the typical FucoPol composition (37mol% fucose, 27mol% galactose, 23mol% glucose and 12mol% glucuronic acid, with an acyl groups content of 13wt%). The average molecular weight was 4.4×10 6 Da and the polydispersity index was 1.2. This study demonstrated that out-of-specification tomato paste is a suitable low-cost substrate for the production of FucoPol, thus providing a route for the valorization of this by-product into a high-value microbial product. Copyright © 2016 Elsevier Ltd. All rights reserved.

31 CFR 360.36 - Payment during life of sole owner.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 31 Money and Finance:Treasury 2 2011-07-01 2011-07-01 false Payment during life of sole owner. 360.36 Section 360.36 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued... UNITED STATES SAVINGS BONDS, SERIES I General Provisions for Payment § 360.36 Payment during life of sole...

The use of fermentation liquid of wastewater primary sedimentation sludge as supplemental carbon source for denitrification based on enhanced anaerobic fermentation.

PubMed

Liu, Feng; Tian, Yu; Ding, Yi; Li, Zhipeng

2016-11-01

Wastewater primary sedimentation sludge was prepared into fermentation liquid as denitrification carbon source, and the main components of fermentation liquid was short-chain volatile fatty acids. Meanwhile, the acetic acid and propionic acid respectively accounted for about 29.36% and 26.56% in short-chain volatile fatty acids. The performance of fermentation liquid, methanol, acetic acid, propionic acid and glucose used as sole carbon source were compared. It was found that the denitrification rate with fermentation liquid as carbon source was 0.17mgNO3(-)-N/mg mixed liquor suspended solid d, faster than that with methanol, acetic acid, and propionic acid as sole carbon source, and lower than that with glucose as sole carbon source. For the fermentation liquid as carbon source, the transient accumulation of nitrite was insignificantly under different initial total nitrogen concentration. Therefore, the use of fermentation liquid for nitrogen removal could improve denitrification rate, and reduce nitrite accumulation in denitrification process. Copyright © 2016 Elsevier Ltd. All rights reserved.

Dynamics of the microbial community during continuous methane fermentation in continuously stirred tank reactors.

PubMed

Tang, Yue-Qin; Shigematsu, Toru; Morimura, Shigeru; Kida, Kenji

2015-04-01

Methane fermentation is an attractive technology for the treatment of organic wastes and wastewaters. However, the process is difficult to control, and treatment rates and digestion efficiency require further optimization. Understanding the microbiology mechanisms of methane fermentation is of fundamental importance to improving this process. In this review, we summarize the dynamics of microbial communities in methane fermentation chemostats that are operated using completely stirred tank reactors (CSTRs). Each chemostat was supplied with one substrate as the sole carbon source. The substrates include acetate, propionate, butyrate, long-chain fatty acids, glycerol, protein, glucose, and starch. These carbon sources are general substrates and intermediates of methane fermentation. The factors that affect the structure of the microbial community are discussed. The carbon source, the final product, and the operation conditions appear to be the main factors that affect methane fermentation and determine the structure of the microbial community. Understanding the structure of the microbial community during methane fermentation will guide the design and operation of practical wastewater treatments. Copyright © 2014 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

31 CFR 315.36 - Payment during life of sole owner.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 31 Money and Finance:Treasury 2 2011-07-01 2011-07-01 false Payment during life of sole owner. 315.36 Section 315.36 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued... § 315.36 Payment during life of sole owner. A savings bond registered in single ownership form (i.e...

Time-based comparative transcriptomics in engineered xylose-utilizing Saccharomyces cerevisiae identifies temperature-responsive genes during ethanol production.

PubMed

Ismail, Ku Syahidah Ku; Sakamoto, Takatoshi; Hasunuma, Tomohisa; Kondo, Akihiko

2013-09-01

Agricultural residues comprising lignocellulosic materials are excellent sources of pentose sugar, which can be converted to ethanol as fuel. Ethanol production via consolidated bioprocessing requires a suitable microorganism to withstand the harsh fermentation environment of high temperature, high ethanol concentration, and exposure to inhibitors. We genetically enhanced an industrial Saccharomyces cerevisiae strain, sun049, enabling it to uptake xylose as the sole carbon source at high fermentation temperature. This strain was able to produce 13.9 g/l ethanol from 50 g/l xylose at 38 °C. To better understand the xylose consumption ability during long-term, high-temperature conditions, we compared by transcriptomics two fermentation conditions: high temperature (38 °C) and control temperature (30 °C) during the first 12 h of fermentation. This is the first long-term, time-based transcriptomics approach, and it allowed us to discover the role of heat-responsive genes when xylose is the sole carbon source. The results suggest that genes related to amino acid, cell wall, and ribosomal protein synthesis are down-regulated under heat stress. To allow cell stability and continuous xylose uptake in order to produce ethanol, hexose transporter HXT5, heat shock proteins, ubiquitin proteins, and proteolysis were all induced at high temperature. We also speculate that the strong relationship between high temperature and increased xylitol accumulation represents the cell's mechanism to protect itself from heat degradation.

Engineering of Corynebacterium glutamicum for growth and succinate production from levoglucosan, a pyrolytic sugar substrate.

PubMed

Kim, Eun-Mi; Um, Youngsoon; Bott, Michael; Woo, Han Min

2015-10-01

Thermochemical processing provides continuous production of bio-oils from lignocellulosic biomass. Levoglucosan, a pyrolytic sugar substrate C6H10O5 in a bio-oil, has been used for ethanol production using engineered Escherichia coli. Here we provide the first example for succinate production from levoglucosan with Corynebacterium glutamicum, a well-known industrial amino acid producer. Heterologous expression of a gene encoding a sugar kinase from Lipomyces starkeyi, Gibberella zeae or Pseudomonas aeruginosa was employed for levoglucosan conversion in C. glutamicum because the wild type was unable to utilize levoglucosan as sole carbon source. As result, expression of a levoglucosan kinase (LGK) of L. starkeyi only enabled growth with levoglucosan as sole carbon source in CgXII minimal medium by catalyzing conversion of levoglucosan to glucose-6-phosphate. Subsequently, the lgk gene was expressed in an aerobic succinate producer of C. glutamicum, strain BL-1. The recombinant strain showed a higher succinate yield (0.25 g g(-1)) from 2% (w/v) levoglucosan than the reference strain BL-1 from 2% (w/v) glucose (0.19 g g(-1)), confirming that levoglucosan is an attractive carbon substrate for C. glutamicum producer strains. In summary, we demonstrated that a pyrolytic sugar could be a potential carbon source for microbial cell factories. © FEMS 2015. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Appetite during consumption of enteral formula as a sole source of nutrition: the effect of supplementing pea-fibre and fructo-oligosaccharides.

PubMed

Whelan, Kevin; Efthymiou, Loukia; Judd, Patricia A; Preedy, Victor R; Taylor, Moira A

2006-08-01

Liquid enteral formulas are commonly used as a sole source of nutritional support of patients in hospital and community settings. Their effect on appetite has important consequences for dietary management of such patients and is likely to be affected by the formula composition. The aim of the present study was to compare appetite within healthy subjects consuming both a standard formula and one supplemented with pea-fibre (10 g/l) and fructo-oligosaccharide (FOS; 5 g/l) as a sole source of nutrition. Eleven healthy subjects consumed a standard formula or a pea-fibre/FOS formula as a sole source of nutrition for 14 d in a double-blind, cross-over trial. Appetite was recorded using standard 100 mm lines anchored at each end by a phrase denoting the most extreme appetite sensation. Consumption of the pea-fibre/FOS formula resulted in higher mean fullness (46 v. 37 mm, P=0.035), minimum fullness (13 v. 9 mm, P=0.024) and minimum satiety (12 v. 8 mm, P=0.012) compared to the standard formula. As there were no differences in macronutrient intake between formulas, these differences are likely to be due to supplementation with pea-fibre and FOS. The effect on appetite of the composition of an enteral formula, both with respect to nutrient content and functional components such as pea-fibre and FOS, may be an important aspect to consider in the dietary management of patients consuming enteral formula as a sole source of nutrition.

[Effects of carbon sources changes on the property and morphology of 2,4-D degraded aerobic sludge granules].

PubMed

Ma, Jing-Yun; Quan, Xian-Chun; Xiong, Wei-Cong

2010-11-01

This study investigated the changes of the morphology, structure, and capability of removing the target contamination of the aerobic granules pre-cultured with mixed substrates of glucose and 2,4-dichlorophenoxyacetic acid (2,4-D) in a long-time running sequence batch reactor (SBR), when the carbon source transformed into the sole carbon source of 2,4-D. Results showed that when the substrate turned to the sole carbon source of 2,4-D, the aerobic granules still maintained a strong degradation ability to the target contamination; a 2,4-D removal percentage of 99.2% -100% and an average COD removal rate of 85.6% were achieved at the initial 2,4-D concentration of 361-564 mg/L. Carbon source transformation caused certain damages to the original aerobic granule structure, made some parts of granules disintegrated, and led to granule size decline from 513 microm to 302 microm. However, those granules maintained the main body, re-aggregated and grew after a period of adaptation due to their strong resistance to toxicity. Aerobic granules capable of utilizing 2,4-D as the sole carbon source with a good settling ability (SYI 20-40 mL/g) and a mean diameter of 489 microm were finally obtained in this study. Scanning electron microscope (SEM) observation showed that the diversity of granule microbial species was declined when turned to the sole carbon source.

Production performance and milk fatty acids profile in grazing dairy cows offered ground corn or liquid molasses as the sole supplemental nonstructural carbohydrate source

USDA-ARS?s Scientific Manuscript database

The objective of this study was to compare the effects of corn meal or liquid molasses fed as the sole supplemental nonstructural carbohydrate source on milk yield and composition, milk fatty acids, and N use efficiency in grazing dairy cows. Ten multiparous organically-certified Jersey cows averagi...

Public funding for contraceptive, sterilization and abortion services, 1994.

PubMed

Sollom, T; Gold, R B; Saul, R

1996-01-01

In 1994, federal and state funding for contraceptive services and supplies reached +715 million. Funding totaled +148 million for contraceptive sterilization and +90 million for abortion services. According to a survey of state health, Medicaid and social service agencies, reported spending on contraceptive services and supplies increased by 11% between 1992 and 1994. In the same period, spending under Title X rose by 37%, making it the third largest public funding source for contraceptive services and supplies. The largest source of public funds for family planning services continues to be the joint federal-state Medicaid program. Medicaid family planning expenditures increased by only 4% between 1992 and 1994, a sizable decrease in growth from previous years. State funds continue to be the second largest source, providing almost one-quarter of reported public expenditures in 1994. The maternal and child health and social services block grants remain relatively minor sources of support nationally, although in a handful of states they provide the majority of public-sector funds. State governments were virtually the sole source of public support for the 203,200 abortions provided in 1994 to low-income women. Despite the loosening of federal abortion funding criteria in FY 1994 permitting payment in cases of rape and incest, federally funded abortions numbered only 282.

5 CFR 890.1048 - Waiver of debarment for a provider that is the sole source of health care services in a community.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 5 Administrative Personnel 2 2010-01-01 2010-01-01 false Waiver of debarment for a provider that... HEALTH BENEFITS PROGRAM Administrative Sanctions Imposed Against Health Care Providers Exceptions to the Effect of Debarments § 890.1048 Waiver of debarment for a provider that is the sole source of health care...

Metabolic behavior and enzymatic aspects of denitrifying EBPR sludge in a continuous-flow anaerobic-anoxic system.

PubMed

Zafiriadis, Ilias; Ntougias, Spyridon; Kapagiannidis, Anastasios G; Aivasidis, Alexander

2013-10-01

The metabolic aspects of enhanced biological phosphorus removal (EBPR) were investigated for the first time in a continuous-flow anaerobic-anoxic plant fed with acetate, propionate, or substrates which are involved in the tricarboxylic acid and/or glyoxylate cycle, i.e., fumarate, malate, or oxaloacetate, as the sole carbon source. Although the polyphosphate-accumulating organisms (PAOs) population remained stable with any carbon source examined, no typical EBPR metabolism was observed during fumarate, malate, or oxaloacetate utilization. Specific enzymatic activities related to EBPR were determined in activated sludge homogenates and directly correlated with the nutrient metabolic rates. The experimental results indicated the direct involvement of alkaline phosphatase, pyrophosphatase, and exopolyphosphatase in the denitrifying EBPR process. Metabolic aspects of glyoxylate cycle enzymes are discussed with regard to the biomass anaerobic and anoxic activity. Process performance was highly influenced by the kind of substrate utilized, indicating that specific metabolic pathways should be followed to favor efficient EBPR.

Diversity of H2/CO2-utilizing acetogenic bacteria from feces of non-methane-producing humans.

PubMed

Bernalier, A; Rochet, V; Leclerc, M; Doré, J; Pochart, P

1996-08-01

The purpose of this work was to study H2/CO2-utilizing acetogenic population in the colons of non-methane-producing individuals harboring low numbers of methanogenic archaea. Among the 50 H2-consuming acetogenic strains isolated from four fecal samples and an in vitro semi-continuous culture enrichment, with H2/CO2 as sole energy source, 20 were chosen for further studies. All isolates were Gram-positive strict anaerobes. Different morphological types were identified, providing evidence of generic diversity. All acetogenic strains characterized used H2/CO2 to form acetate as the sole metabolite, following the stoichiometric equation of reductive acetogenesis. These bacteria were also able to use a variety of organic compounds for growth. The major end product of glucose fermentation was acetate, except for strains of cocci that mainly produced lactate. Yeast extract was not necessary, but was stimulatory for growth and acetogenesis from H2/CO2.

Continuous ethanol fermentation of lactose by a recombinant flocculating Saccharomyces cerevisiae strain

DOE Office of Scientific and Technical Information (OSTI.GOV)

Domingues, L.; Dantas, M.M.; Lima, N.

1999-09-20

Alcohol fermentation of lactose was investigated using a recombinant flocculating Saccharomyces cetevisiae, expressing the LAC4 (coding the {beta}-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces marxianus. Data on yeast fermentation and growth on a medium containing lactose as the sole carbon source are presented. In the range of studied lactose concentrations, total lactose consumption was observed with a conversion yield of ethanol close to the expected theoretical value. For the continuously operating bioreactor, an ethanol productivity of 11 g L{sup {minus}1} h{sup {minus}1} (corresponding to a feed lactose concentration of 50 g L{sup {minus}1} and a dilution ratemore » of 0.55 h{sup {minus}1}) was obtained, which is 7 times larger than the continuous conventional systems. The system stability was confirmed by keeping it in operation for 6 months.« less

Intelligence Sharing in Counterproliferation

DTIC Science & Technology

2007-09-01

Claims Alleged Mobile WMD Plants Solely for ‘ Agrochemicals ,” Spiegel Online, Hamburg, Germany. Translated by OpenSource.gov https...Knauer, Sebastian. "German Site Claims Alleged Mobile WMD Plants Solely for Agrochemicals ." Spiegel Online. https://www.opensource.gov (accessed June

Role of L-lysine-alpha-ketoglutarate aminotransferase in catabolism of lysine as a nitrogen source for Rhodotorula glutinis.

PubMed Central

Kinzel, J J; Winston, M K; Bhattacharjee, J K

1983-01-01

Wild-type and saccharopine dehydrogenaseless mutant strains of Rhodotorula glutinis grew in minimal medium containing lysine as the sole nitrogen source and simultaneously accumulated, in the culture supernatant, large amounts of a product identified as alpha-aminoadipic-delta-semialdehyde. The saccharopine dehydrogenase and pipecolic acid oxidase levels remained unchanged in wild-type cells grown in the presence of ammonium or lysine as the nitrogen source. Lysine-alpha-ketoglutarate aminotransferase activity was demonstrated in ammonium-grown cells. This activity was depressed in cells grown in the presence of lysine as the sole source of nitrogen. PMID:6408065

Continuous EEG source imaging enhances analysis of EEG-fMRI in focal epilepsy.

PubMed

Vulliemoz, S; Rodionov, R; Carmichael, D W; Thornton, R; Guye, M; Lhatoo, S D; Michel, C M; Duncan, J S; Lemieux, L

2010-02-15

EEG-correlated fMRI (EEG-fMRI) studies can reveal haemodynamic changes associated with Interictal Epileptic Discharges (IED). Methodological improvements are needed to increase sensitivity and specificity for localising the epileptogenic zone. We investigated whether the estimated EEG source activity improved models of the BOLD changes in EEG-fMRI data, compared to conventional < event-related > designs based solely on the visual identification of IED. Ten patients with pharmaco-resistant focal epilepsy underwent EEG-fMRI. EEG Source Imaging (ESI) was performed on intra-fMRI averaged IED to identify the irritative zone. The continuous activity of this estimated IED source (cESI) over the entire recording was used for fMRI analysis (cESI model). The maps of BOLD signal changes explained by cESI were compared to results of the conventional IED-related model. ESI was concordant with non-invasive data in 13/15 different types of IED. The cESI model explained significant additional BOLD variance in regions concordant with video-EEG, structural MRI or, when available, intracranial EEG in 10/15 IED. The cESI model allowed better detection of the BOLD cluster, concordant with intracranial EEG in 4/7 IED, compared to the IED model. In 4 IED types, cESI-related BOLD signal changes were diffuse with a pattern suggestive of contamination of the source signal by artefacts, notably incompletely corrected motion and pulse artefact. In one IED type, there was no significant BOLD change with either model. Continuous EEG source imaging can improve the modelling of BOLD changes related to interictal epileptic activity and this may enhance the localisation of the irritative zone. Copyright 2009 Elsevier Inc. All rights reserved.

Aerobic biodegradation of the sulfonamide antibiotic sulfamethoxazole by activated sludge applied as co-substrate and sole carbon and nitrogen source.

PubMed

Müller, Elisabeth; Schüssler, Walter; Horn, Harald; Lemmer, Hilde

2013-08-01

Potential aerobic biodegradation mechanisms of the widely used polar, low-adsorptive sulfonamide antibiotic sulfamethoxazole (SMX) were investigated in activated sludge at bench scale. The study focused on (i) SMX co-metabolism with acetate and ammonium nitrate and (ii) SMX utilization when present as the sole carbon and nitrogen source. With SMX adsorption being negligible, elimination was primarily based on biodegradation. Activated sludge was able to utilize SMX both as a carbon and/or nitrogen source. SMX biodegradation was enhanced when a readily degradable energy supply (acetate) was provided which fostered metabolic activity. Moreover, it was raised under nitrogen deficiency conditions. The mass balance for dissolved organic carbon showed an incomplete SMX mineralization with two scenarios: (i) with SMX as a co-substrate, 3-amino-5-methyl-isoxazole represented the main stable metabolite and (ii) SMX as sole carbon and nitrogen source possibly yielded hydroxyl-N-(5-methyl-1,2-oxazole-3-yl)benzene-1-sulfonamide as a further metabolite. Copyright © 2013 Elsevier Ltd. All rights reserved.

Differential Growth Responses of Marine Phytoplankton to Herbicide Glyphosate

PubMed Central

Wang, Cong; Lin, Xin; Li, Ling; Lin, Senjie

2016-01-01

Glyphosate is a globally popular herbicide to kill weeds and its wide applications may lead to accumulation in coastal oceans as a source of phosphorus (P) nutrient or growth inhibitor of phytoplankton. We studied the physiological effects of glyphosate on fourteen species representing five major coastal phytoplankton phyla (haptophyta, bacillariophyta, dinoflagellata, raphidophyta, and chlorophyta). Based on growth responses to different concentrations of glyphosate under contrasting dissolved inorganic phosphorus (DIP) conditions, we found that phytoplankton species could be classified into five groups. Group I (Emiliania huxleyi, Skeletonema costatum, Phaeodactylum tricornutum) could utilize glyphosate as sole P-source to support growth in axenic culture, but in the presence of DIP, they were inhibited by both 36-μM and 360-μM glyphosate. Group II (Karenia mikimotoi, Prorocentrum minimum, Dunaliella tertiolecta, Symbiodinium sp., Heterosigma akashiwo and Alexandrium catenella) could not utilize glyphosate as sole P-source to support growth, and in the presence of DIP growth was not affected by 36-μM but inhibited by 360-μM glyphosate. Glyphosate consistently enhanced growth of Group III (Isochrysis galbana) and inhibited Group IV (Thalassiosira weissflogii, Thalassiosira pseudonana and Chattonella marina) regardless of DIP condition. Group V (Amphidinium carterae) exhibited no measurable response to glyphosate regardless of DIP condition. This grouping is not congruent with the phylogenetic relationships of the phytoplankton species suggesting functional differentiation driven by environmental pressure. We conclude that glyphosate could be used as P-source by some species while is toxic to some other species and yet has no effects on others. The observed differential effects suggest that the continued use of glyphosate and increasing concentration of this herbicide in the coastal waters will likely exert significant impact on coastal marine phytoplankton community structure. PMID:26985828

Aerobic Growth of Escherichia coli with 2,4,6-Trinitrotoluene (TNT) as the Sole Nitrogen Source and Evidence of TNT Denitration by Whole Cells and Cell-Free Extracts▿ †

PubMed Central

Stenuit, Ben; Eyers, Laurent; Rozenberg, Raoul; Habib-Jiwan, Jean-Louis; Agathos, Spiros N.

2006-01-01

Escherichia coli grew aerobically with 2,4,6-trinitrotoluene (TNT) as sole nitrogen source and caused TNT's partial denitration. This reaction was enhanced in nongrowing cell suspensions with 0.516 mol nitrite released per mol TNT. Cell extracts denitrated TNT in the presence of NAD(P)H. Isomers of amino-dimethyl-tetranitrobiphenyl were detected and confirmed with U-15N-labeled TNT. PMID:17012591

Lean Six Sigma Project - Defense Logistics Agency/Honeywell Long-Term Contract Model Using One-Pass Pricing for Sole-Source Spare Parts

DTIC Science & Technology

2011-02-18

Control Limit Lower Control Limit Reaction Plan 1 Complaints from other suppliers (synopsis, award) SCG During award process Identify Sole- Source...Parts 0.0 1.0 0.0 Evaluate complaint, if valid remove item from contract. 2 Tracking timeline for procurement/reviews SCG During pre- award process...Review Solicitation 100.0 Determine where the document stands in the approval process. Adjust milestones and followup . 3 FAR/DPAP guidance SCG

Parameter-space metric of semicoherent searches for continuous gravitational waves

NASA Astrophysics Data System (ADS)

Pletsch, Holger J.

2010-08-01

Continuous gravitational-wave (CW) signals such as emitted by spinning neutron stars are an important target class for current detectors. However, the enormous computational demand prohibits fully coherent broadband all-sky searches for prior unknown CW sources over wide ranges of parameter space and for yearlong observation times. More efficient hierarchical “semicoherent” search strategies divide the data into segments much shorter than one year, which are analyzed coherently; then detection statistics from different segments are combined incoherently. To optimally perform the incoherent combination, understanding of the underlying parameter-space structure is requisite. This problem is addressed here by using new coordinates on the parameter space, which yield the first analytical parameter-space metric for the incoherent combination step. This semicoherent metric applies to broadband all-sky surveys (also embedding directed searches at fixed sky position) for isolated CW sources. Furthermore, the additional metric resolution attained through the combination of segments is studied. From the search parameters (sky position, frequency, and frequency derivatives), solely the metric resolution in the frequency derivatives is found to significantly increase with the number of segments.

31 CFR 536.304 - Foreign person.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 31 Money and Finance:Treasury 3 2013-07-01 2013-07-01 false Foreign person. 536.304 Section 536.304 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) OFFICE OF... organized solely under the laws of the United States or existing solely in the United States, but does not...

31 CFR 536.304 - Foreign person.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 31 Money and Finance:Treasury 3 2011-07-01 2011-07-01 false Foreign person. 536.304 Section 536.304 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) OFFICE OF... organized solely under the laws of the United States or existing solely in the United States, but does not...

31 CFR 595.304 - Foreign person.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 31 Money and Finance:Treasury 3 2011-07-01 2011-07-01 false Foreign person. 595.304 Section 595.304 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) OFFICE OF... organized solely under the laws of the United States or existing solely in the United States, but does not...

31 CFR 594.304 - Foreign person.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 31 Money and Finance:Treasury 3 2011-07-01 2011-07-01 false Foreign person. 594.304 Section 594.304 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) OFFICE OF... organized solely under the laws of the United States or existing solely in the United States, but does not...

31 CFR 536.304 - Foreign person.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 31 Money and Finance:Treasury 3 2014-07-01 2014-07-01 false Foreign person. 536.304 Section 536.304 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) OFFICE OF... organized solely under the laws of the United States or existing solely in the United States, but does not...

31 CFR 536.304 - Foreign person.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Foreign person. 536.304 Section 536.304 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) OFFICE OF... organized solely under the laws of the United States or existing solely in the United States, but does not...

The 8-year report on MSX thermal blanket outgassing: an inexhaustible reservoir

NASA Astrophysics Data System (ADS)

Boies, Mark T.; Green, B. David; Galica, Gary E.; Uy, O. Manuel; Benson, Richard C.; Silver, David M.; Wood, Bobby E.; Lesho, Jeffrey C.; Hall, David F.; Dyer, James S.

2004-10-01

The Midcourse Space Experiment (MSX) spacecraft has now been in orbit for over 8 years. The Total Pressure Sensor (TPS) has continually made measurements of the ambient and spacecraft induced environment since launch on April 24, 1996. The environment surrounding the satellite continues to be very dynamic. The magnitude of the solar induced pressure peaks has not decayed with time as would be expected. Even sole consideration of measured pressure versus duration of solar illumination does not illustrate long term decay. The TPS has continued to measure pressure variations of more than 2 orders of magnitude as the sun illuminates the blankets surrounding the instrument. The multi-layer thermal blankets appear to represent a large virtual source of water. The measured pressure is highly dependent upon the solar angle and the shadowing of surfaces by other structures. We will focus our attention on the results from an identical set of experiments conducted each year in July.

31 CFR 353.36 - Payment during life of sole owner.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 31 Money and Finance:Treasury 2 2011-07-01 2011-07-01 false Payment during life of sole owner. 353.36 Section 353.36 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued... UNITED STATES SAVINGS BONDS, SERIES EE AND HH General Provisions for Payment § 353.36 Payment during life...

40 CFR 1045.620 - What are the provisions for exempting engines used solely for competition?

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 34 2012-07-01 2012-07-01 false What are the provisions for exempting engines used solely for competition? 1045.620 Section 1045.620 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR POLLUTION CONTROLS CONTROL OF EMISSIONS FROM SPARK-IGNITION PROPULSION...

40 CFR 1045.620 - What are the provisions for exempting engines used solely for competition?

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 34 2013-07-01 2013-07-01 false What are the provisions for exempting engines used solely for competition? 1045.620 Section 1045.620 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR POLLUTION CONTROLS CONTROL OF EMISSIONS FROM SPARK-IGNITION PROPULSION...

40 CFR 1045.620 - What are the provisions for exempting engines used solely for competition?

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 33 2011-07-01 2011-07-01 false What are the provisions for exempting engines used solely for competition? 1045.620 Section 1045.620 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR POLLUTION CONTROLS CONTROL OF EMISSIONS FROM SPARK-IGNITION PROPULSION...

40 CFR 1045.620 - What are the provisions for exempting engines used solely for competition?

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 33 2014-07-01 2014-07-01 false What are the provisions for exempting engines used solely for competition? 1045.620 Section 1045.620 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR POLLUTION CONTROLS CONTROL OF EMISSIONS FROM SPARK-IGNITION PROPULSION...

40 CFR 1045.620 - What are the provisions for exempting engines used solely for competition?

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 32 2010-07-01 2010-07-01 false What are the provisions for exempting engines used solely for competition? 1045.620 Section 1045.620 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR POLLUTION CONTROLS CONTROL OF EMISSIONS FROM SPARK-IGNITION PROPULSION...

[Kinetic simulation of enhanced biological phosphorus removal with fermentation broth as carbon source].

PubMed

Zhang, Chao; Chen, Yin-Guang

2013-07-01

As a high-quality carbon source, fermentation broth could promote the phosphorus removal efficiency in enhanced biological phosphorus removal (EBPR). The transformation of substrates in EBPR fed with fermentation broth was well simulated using the modified activated sludge model No. 2 (ASM2) based on the carbon source metabolism. When fermentation broth was used as the sole carbon source, it was found that heterotrophic bacteria acted as a promoter rather than a competitor to the phosphorus accumulating organisms (PAO). When fermentation broth was used as a supplementary carbon source of real municipal wastewater, the wastewater composition was optimized for PAO growth; and the PAO concentration, which was increased by 3.3 times compared to that in EBPR fed with solely real municipal wastewater, accounting for about 40% of the total biomass in the reactor.

Glutamate Dehydrogenase from Apodachlya (Oomycetes) 1

PubMed Central

Price, Jeffrey S.; Gleason, Frank H.

1972-01-01

A glutamate dehydrogenase specific for nicotinamide-adenine-dinucleotide has been purified 50-fold from Apodachlya brachynema (Leptomitales). Certain physical, chemical, and kinetic properties of this enzyme have been studied, particularly specificity for coenzymes and substrates. With glucose as the sole carbon source, the synthesis of glutamate dehydrogenase was repressed, whereas glutamate, proline, alanine, or ornithine plus aspartate as sole carbon sources induced synthesis of the enzyme. These data indicate that the function of this enzyme is primarily degradative, although there is no evidence for a nicotinamide-adenine-dinucleotide-phosphate-specific biosynthetic glutamate dehydrogenase in Apodachlya. PMID:16657902

Concussion under-reporting and pressure from coaches, teammates, fans, and parents.

PubMed

Kroshus, Emily; Garnett, Bernice; Hawrilenko, Matt; Baugh, Christine M; Calzo, Jerel P

2015-06-01

Concussions from sport present a substantial public health burden given the number of youth, adolescent and emerging adult athletes that participate in contact or collision sports. Athletes who fail to report symptoms of a suspected concussion and continue play are at risk of worsened symptomatology and potentially catastrophic neurologic consequences if another impact is sustained during this vulnerable period. Understanding why athletes do or do not report their symptoms is critical for developing efficacious strategies for risk reduction. Psychosocial theories and frameworks that explicitly incorporate context, as a source of expectations about the outcomes of reporting and as a source of behavioral reinforcement, are useful in framing this problem. The present study quantifies the pressure that athletes experience to continue playing after a head impact--from coaches, teammates, parents, and fans--and assesses how this pressure, both independently and as a system, is related to future concussion reporting intention. Participants in the study were 328 male and female athletes from 19 teams competing in one of seven sports (soccer, lacrosse, basketball, softball, baseball, volleyball, field hockey) at four colleges in the northeast region of the United States. Results found that more than one-quarter of the sample had experienced pressure from at least one source to continue playing after a head impact during the previous year. Results of a latent profile mixture model indicated that athletes who experienced pressure from all four of the measured sources were significantly more likely to intend to continue playing in the future than were athletes who had not experienced pressure from all sources, or only pressure from coaches and teammates. These findings underscore the importance of designing interventions that address the system in which athletes make decisions about concussion reporting, including athletes' parents, rather than focusing solely on modifying the individual's reporting cognitions. Copyright © 2015 Elsevier Ltd. All rights reserved.

Concussion under-reporting and pressure from coaches, teammates, fans, and parents

PubMed Central

Kroshus, Emily; Garnett, Bernice; Hawrilenko, Matt; Baugh, Christine M.; Calzo, Jerel P.

2015-01-01

Concussions from sport present a substantial public health burden given the number of youth, adolescent and emerging adult athletes that participate in contact or collision sports. Athletes who fail to report symptoms of a suspected concussion and continue play are at risk of worsened symptomatology and potentially catastrophic neurologic consequences if another impact is sustained during this vulnerable period. Understanding why athletes do or do not report their symptoms is critical for developing efficacious strategies for risk reduction. Psychosocial theories and frameworks that explicitly incorporate context, as a source of expectations about the outcomes of reporting and as a source of behavioral reinforcement, are useful in framing this problem. The present study quantifies the pressure that athletes experience to continue playing after a head impact—from coaches, teammates, parents, and fans—and assesses how this pressure, both independently and as a system, is related to future concussion reporting intention. Participants in the study were 328 male and female athletes from 19 teams competing in one of seven sports (soccer, lacrosse, basketball, softball, baseball, volleyball, field hockey) at four colleges in the northeast region of the United States. Results found that more than one-quarter of the sample had experienced pressure from at least one source to continue playing after a head impact during the previous year. Results of a latent profile mixture model indicated that athletes who experienced pressure from all four of the measured sources were significantly more likely to intend to continue playing in the future than were athletes who had not experienced pressure from all sources, or only pressure from coaches and teammates. These findings underscore the importance of designing interventions that address the system in which athletes make decisions about concussion reporting, including athletes’ parents, rather than focusing solely on modifying the individual’s reporting cognitions. PMID:25917137

Release of somatic embryogenic potential from excised zygotic embryos of carrot and maintenance of proembryonic cultures in hormone-free medium.

PubMed

Smith, D L; Krikorian, A D

1989-01-01

Excised zygotic embryos, mericarps ("seeds") and hypocotyls of seedlings of cultivated carrot Daucus carota cv. Scarlet Nantes were evaluated for their ability to generate somatic embryos on a semisolid hormone-free nutrient medium. Neither intact zygotic embryos nor hypocotyls ever produced somatic embryos. However, mericarps and broken zygotic embryos were excellent sources for somatic embryo production (response levels as high as 86%). Somatic embryo formation was highest from cotyledons, but was also observed on isolated hypocotyls and root tips of mature zygotic embryos. On media containing unreduced nitrogen, somatic embryo formation led to the generation of vigorous cultures comprised entirely of somatic embryos at various stages of development which in turn proliferated still other somatic embryos. However, a medium was devised which when 1-5 mM NH4+ was the sole nitrogen source, led only to a proliferation of globular proembryos. Sustained subculturing of these proembryos at 2-3 week intervals enabled establishment of highly uniform cultures in which no further development into more mature stages of embryonic development occurred. These have been maintained, without decline, as morphogenetically competent proembryonic globules for over ten months. A basal medium containing from 1-5 mM NH4+ as the sole nitrogen source appears not to be inductive to somatic proembryo formation. Instead, such a medium is best thought of as permissive to the expression of embryogenically determined cells within zygotic embryos. By excising and breaking or wounding zygotic embryos, constituent cells are probably released from positional or chemical restraints and thus are able to express their innate embryogenic potential. Once a proembryonic culture is established, this medium containing 1-5 mM NH4+ as the sole nitrogen source provides a nonpermissive environment to the development and growth of later embryonic stages, but it does allow the continued formation and multiplication of globular somatic proembryos. The sequence of events leading from excised broken zygotic embryos to the formation of somatic embryos and the maintenance of somatic proembryos are demonstrated by scanning electron microscopy and histological preparations. Germination levels from intact zygotic embryos on media with varying levels and ratios of unreduced vs. reduced inorganic nitrogen were determined as well and provided baseline or control data on the type of response obtained from nonwounded material.

Release of somatic embryogenic potential from excised zygotic embryos of carrot and maintenance of proembryonic cultures in hormone-free medium

NASA Technical Reports Server (NTRS)

Smith, D. L.; Krikorian, A. D.

1989-01-01

Excised zygotic embryos, mericarps ("seeds") and hypocotyls of seedlings of cultivated carrot Daucus carota cv. Scarlet Nantes were evaluated for their ability to generate somatic embryos on a semisolid hormone-free nutrient medium. Neither intact zygotic embryos nor hypocotyls ever produced somatic embryos. However, mericarps and broken zygotic embryos were excellent sources for somatic embryo production (response levels as high as 86%). Somatic embryo formation was highest from cotyledons, but was also observed on isolated hypocotyls and root tips of mature zygotic embryos. On media containing unreduced nitrogen, somatic embryo formation led to the generation of vigorous cultures comprised entirely of somatic embryos at various stages of development which in turn proliferated still other somatic embryos. However, a medium was devised which when 1-5 mM NH4+ was the sole nitrogen source, led only to a proliferation of globular proembryos. Sustained subculturing of these proembryos at 2-3 week intervals enabled establishment of highly uniform cultures in which no further development into more mature stages of embryonic development occurred. These have been maintained, without decline, as morphogenetically competent proembryonic globules for over ten months. A basal medium containing from 1-5 mM NH4+ as the sole nitrogen source appears not to be inductive to somatic proembryo formation. Instead, such a medium is best thought of as permissive to the expression of embryogenically determined cells within zygotic embryos. By excising and breaking or wounding zygotic embryos, constituent cells are probably released from positional or chemical restraints and thus are able to express their innate embryogenic potential. Once a proembryonic culture is established, this medium containing 1-5 mM NH4+ as the sole nitrogen source provides a nonpermissive environment to the development and growth of later embryonic stages, but it does allow the continued formation and multiplication of globular somatic proembryos. The sequence of events leading from excised broken zygotic embryos to the formation of somatic embryos and the maintenance of somatic proembryos are demonstrated by scanning electron microscopy and histological preparations. Germination levels from intact zygotic embryos on media with varying levels and ratios of unreduced vs. reduced inorganic nitrogen were determined as well and provided baseline or control data on the type of response obtained from nonwounded material.

Special role of corn flour as an ideal carbon source for aerobic denitrification with minimized nitrous oxide emission.

PubMed

Zhu, Shuangyue; Zheng, Maosheng; Li, Can; Gui, Mengyao; Chen, Qian; Ni, Jinren

2015-06-01

Much effort has been made for reducing nitrous oxide (N2O) emission in wastewater treatment processes. This paper presents an interesting way to minimize N2O in aerobic denitrification by strain Pseudomonas stutzeri PCN-1 with help of corn flour as cheaper additional carbon source. Experimental results showed that maximal N2O accumulation by strain PCN-1 was only 0.02% of removed nitrogen if corn flour was used as sole carbon source, which was significantly reduced by 52.07-99.81% comparing with others such as succinate, glucose, acetate and citrate. Sustained release of reducing sugar from starch and continuous expression of nosZ coding for N2O reductase contributed to the special role of corn flour as the ideal carbon source for strain PCN-1. Further experiments in sequencing batch reactors (SBRs) demonstrated similarly efficient nitrogen removal with much less N2O emission due to synergy of the novel strain and activated sludge, which was then confirmed by quantitative PCR analysis. Copyright © 2015 Elsevier Ltd. All rights reserved.

Breastfeeding as the sole source of milk for 6 months and adolescent bone mineral density.

PubMed

Blanco, E; Burrows, R; Reyes, M; Lozoff, B; Gahagan, S; Albala, C

2017-10-01

Little is known regarding the relationship between early life factors and bone mineral density (BMD). We found a positive association between breastfeeding for at least 6 months, without formula supplementation, and whole body adolescent BMD z-score. The aim of the study is to assess the role of breastfeeding BF on adolescent bone mineral density (BMD) in a cohort prospectively followed since infancy. We studied 679 participants from an infancy iron deficiency anemia preventive trial in Santiago, Chile, followed to adolescence. Breast and bottle feeding were ascertained weekly from 4 to 12 months. At 16 years, whole body BMD was assessed by DEXA. Using linear regression, we evaluated associations between BF duration and BF as the sole source of milk and adolescent BMD z-score, adjusting for possible infancy, adolescent, and background confounders. Mean birth weight and length were 3.5 (0.3) kg and 50.7 (1.6) cm. For at least 6 months, BF was the sole source of milk for 26.3% and with supplementation for 36.7%. For 37%, BF was provided for less than 6 months. Mean 16-year BMD z-score was 0.25 (1.0). Covariates included male sex, birth length, and gestational age. BF as the sole source of milk ≥6 months, compared to BF < 6 months, was associated with higher adolescent BMD z-score adjusting for covariates (β = 0.29, p < 0.05). Mixed BF was not significantly related to adolescent BMD z-score (β = 0.06, p = 0.47). For every 30 days of BF as the sole source of milk, adolescent BMD z-score increased by 0.03 (p = 0.01). BF without formula supplementation for at least 6 months was associated with higher adolescent BMD z-score and a suggestive trend in the same direction for BMD suggests that exclusivity and duration of BF may play a role in adolescent bone health.

49 CFR 372.103 - Motor vehicles employed solely in transporting school children and teachers to or from school.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 5 2014-10-01 2014-10-01 false Motor vehicles employed solely in transporting school children and teachers to or from school. 372.103 Section 372.103 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR CARRIER SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION FEDERAL MOTOR CARRIER SAFETY...

Growth of a Strictly Anaerobic Bacterium on Furfural (2-Furaldehyde)

PubMed Central

Brune, Gerhard; Schoberth, Siegfried M.; Sahm, Hermann

1983-01-01

A strictly anaerobic bacterium was isolated from a continuous fermentor culture which converted the organic constituents of sulfite evaporator condensate to methane and carbon dioxide. Furfural is one of the major components of this condensate. This furfural isolate could degrade furfural as the sole source of carbon and energy in a defined mineral-vitamin-sulfate medium. Acetic acid was the major fermentation product. This organism could also use ethanol, lactate, pyruvate, or fumarate and contained cytochrome c3 and desulfoviridin. Except for furfural degradation, the characteristics of the furfural isolate were remarkably similar to those of the sulfate reducer Desulfovibrio gigas. The furfural isolate has been tentatively identified as Desulfovibrio sp. strain F-1. Images PMID:16346423

Regularized two-step brain activity reconstruction from spatiotemporal EEG data

NASA Astrophysics Data System (ADS)

Alecu, Teodor I.; Voloshynovskiy, Sviatoslav; Pun, Thierry

2004-10-01

We are aiming at using EEG source localization in the framework of a Brain Computer Interface project. We propose here a new reconstruction procedure, targeting source (or equivalently mental task) differentiation. EEG data can be thought of as a collection of time continuous streams from sparse locations. The measured electric potential on one electrode is the result of the superposition of synchronized synaptic activity from sources in all the brain volume. Consequently, the EEG inverse problem is a highly underdetermined (and ill-posed) problem. Moreover, each source contribution is linear with respect to its amplitude but non-linear with respect to its localization and orientation. In order to overcome these drawbacks we propose a novel two-step inversion procedure. The solution is based on a double scale division of the solution space. The first step uses a coarse discretization and has the sole purpose of globally identifying the active regions, via a sparse approximation algorithm. The second step is applied only on the retained regions and makes use of a fine discretization of the space, aiming at detailing the brain activity. The local configuration of sources is recovered using an iterative stochastic estimator with adaptive joint minimum energy and directional consistency constraints.

21 CFR 558.515 - Robenidine.

Code of Federal Regulations, 2014 CFR

2014-04-01

... infection caused by M. gallisepticum and E. coli susceptible to chlortetracycline. Feed continuously as sole... in the reduction of mortality due to E. coli susceptible to chlortetracycline. Feed continuously as... gallisepticum and E. coli susceptible to oxytetracycline. Feed continuously for 7 to 14 days. Do not feed to...

Biodegradation of munitions compounds by a sulfate reducing bacterial enrichment culture

DOE Office of Scientific and Technical Information (OSTI.GOV)

Boopathy, R.; Manning, J.

1997-08-01

The degradation of several munitions compounds was studied. The compounds included 2,4,6-trinitrotoluene (TNT), hexahydro-1,3,5-trinitro-1,3,5-triazine, octahydro-1,3,5,7-tetranitro-1,3,5,7-tetraazocine, 2,4,6-trinitrobenzene (TNB), and 2,4-dinitrotoluene. All of the compounds studied were degraded by the sulfate reducing bacterial (SRB) enrichment culture. The SRB culture did not use the munitions compounds as their sole source of carbon. However, all the munitions compounds tested served as the sole source of nitrogen for the SRB culture. Degradation of munitions compounds was achieved by a co-metabolic process. The SRB culture used a variety of carbon sources including pyruvate, ethanol, formate, lactate, and H{sub 2}-CO{sub 2}. The SRB culture was an incompletemore » oxidizer, unable to carry out the terminal oxidation of organic substrates to CO{sub 2} as the sole product, and it did not use acetate or methanol as a carbon source. In addition to serving as nitrogen sources, the munitions compounds also served as electron acceptors in the absence of sulfate. A soil slurry experiment with 5% and 10% munitions compounds-contaminated soil showed that the contaminant TNT was metabolized by the SRB culture in the presence of pyruvate as electron donor. This culture may be useful in decontaminating munitions compounds-contaminated soil and water under anaerobic conditions.« less

Polymorphism of Malassezia furfur.

PubMed

Salkin, I F; Gordon, M A

1977-04-01

Alterations in the morphologic and physiologic characters of 11 isolates of Pityrosporum orbiculare were noted upon prolonged maintenance in pure culture. Successive subculturing of each isolate resulted in its progressive conversion from globose (P. orbiculare) through ovoid to cylindrical (P. ovale) form. Globose forms utilized neither olive oil nor Tween 20 as a sole carbon source, nor KNO3 as a sole source of nitrogen, while ovoid and cylindrical forms utilized both of these carbon sources, and one of four strains of the cylindrical form assimilated KNO3. These results suggest that P. orbiculare and P. ovale are stages in the complex developmental cycle of a single species (Malassezia furfur), but the three names should be preserved until the life cycle is more fully understood.

Origins and trends in ethane and propane in the United Kingdom from 1993 to 2012

NASA Astrophysics Data System (ADS)

Derwent, R. G.; Field, R. A.; Dumitrean, P.; Murrells, T. P.; Telling, S. P.

2017-05-01

Continuous, high frequency in situ observations of ethane and propane began in the United Kingdom in 1993 and have continued through to the present day at a range of kerbside, urban background and rural locations. Whilst other monitored C2 - C8 hydrocarbons have shown dramatic declines in concentrations by close to or over an order of magnitude, ethane and propane levels have remained at or close to their 1993 values. Urban ethane sources appear to be dominated by natural gas leakage. Background levels of ethane associated with long range transport are rising. However, natural gas leakage is not the sole source of urban propane. Oil and gas operations lead to elevated propane levels in urban centres when important refinery operations are located nearby. Weekend versus weekday average diurnal curves for ethane and propane at an urban background site in London show the importance of natural gas leakage for both ethane and propane, and road traffic sources for propane. The road traffic source of propane was tentatively identified as arising from petrol-engined motor vehicle refuelling and showed a strong downwards trend at the long-running urban background and rural sites. The natural gas leakage source of ethane and propane in the observations exhibits an upwards trend whereas that in the UK emission inventory trends downwards. Also, inventory emissions for natural gas leakage appeared to be significantly underestimated compared with the observations. In addition, the observed ethane to propane ratio found here for natural gas leakage strongly disagreed with the inventory ratio.

Unraveling the sub-processes of selective attention: insights from dynamic modeling and continuous behavior.

PubMed

Frisch, Simon; Dshemuchadse, Maja; Görner, Max; Goschke, Thomas; Scherbaum, Stefan

2015-11-01

Selective attention biases information processing toward stimuli that are relevant for achieving our goals. However, the nature of this bias is under debate: Does it solely rely on the amplification of goal-relevant information or is there a need for additional inhibitory processes that selectively suppress currently distracting information? Here, we explored the processes underlying selective attention with a dynamic, modeling-based approach that focuses on the continuous evolution of behavior over time. We present two dynamic neural field models incorporating the diverging theoretical assumptions. Simulations with both models showed that they make similar predictions with regard to response times but differ markedly with regard to their continuous behavior. Human data observed via mouse tracking as a continuous measure of performance revealed evidence for the model solely based on amplification but no indication of persisting selective distracter inhibition.

40 CFR 69.31 - New exemptions.

Code of Federal Regulations, 2014 CFR

2014-07-01

.... This exemption applies solely to the PSD major source baseline date and trigger date in the... applicable requirement that is triggered by, implemented or calculated from the PSD major source baseline... the Northern Mariana Islands, use January 13, 1997 as the PSD major source baseline date and trigger...

Women Business Owners. Facts on U.S. Working Women. Fact Sheet No. 87-2.

ERIC Educational Resources Information Center

Women's Bureau (DOL), Washington, DC.

From 1977 to 1983, the number of sole proprietorships operated by women increased from 1.9 to 3.3 million. The number of self-employed women--the majority of whom are sole proprietors--continued to grow. In 1982 the median earnings of self-employed women were substantially lower than those of wage and salary women workers and self-employed men.…

48 CFR 619.808-1 - Sole source.

Code of Federal Regulations, 2010 CFR

2010-10-01

... source. (a) If the acquisition is conducted under an MOU cited in 619.800, the 8(a) contractor is... the acquisition is conducted under an MOU cited in 619.800, the agency is delegated the authority to...

CCD photometry of 1218+304 1219+28 and 1727+50: Point sources, associated nebulosity and broadband spectra

NASA Technical Reports Server (NTRS)

Weistrop, D.; Shaffer, D. B.; Mushotzky, R. F.; Reitsma, H. J.; Smith, B. A.

1981-01-01

Visual and far red surface photometry were obtained of two X-ray emitting BL Lacertae objects, 1218+304 (2A1219+305) and 1727+50 (Izw 187), as well as the highly variable object 1219+28 (ON 231, W Com). The intensity distribution for 1727+50 can be modeled using a central point source plus a de Vaucouleurs intensity law for an underlying galaxy. The broad band spectral energy distribution so derived is consistent with what is expected for an elliptical galaxy. The spectral index of the point source is alpha = 0.97. Additional VLBI and X-ray data are also reported for 1727+50. There is nebulosity associated with the recently discovered object 1218+304. No nebulosity is found associated with 1219+28. A comparison of the results with observations at X-ray and radio frequencies suggests that all the emission from 1727+50 and 1218+304 can be interpreted as due solely to direct synchrotron emission. If this is the case, the data further imply the existence of relativistic motion effects and continuous particle injection.

Cutaneous stimulation of discrete regions of the sole during locomotion produces “sensory steering” of the foot

PubMed Central

2014-01-01

Background While the neural and mechanical effects of whole nerve cutaneous stimulation on human locomotion have been previously studied, there is less information about effects evoked by activation of discrete skin regions on the sole of the foot. Electrical stimulation of discrete foot regions evokes position-modulated patterns of cutaneous reflexes in muscles acting at the ankle during standing but data during walking are lacking. Here, non-noxious electrical stimulation was delivered to five discrete locations on the sole of the foot (heel, and medial and lateral sites on the midfoot and forefoot) during treadmill walking. EMG activity from muscles acting at the hip, knee and ankle were recorded along with movement at these three joints. Additionally, 3 force sensing resistors measuring continuous force changes were placed at the heel, and the medial and lateral aspects of the right foot sole. All data were sorted based on stimulus occurrence in twelve step-cycle phases, before being averaged together within a phase for subsequent analysis. Methods Non-noxious electrical stimulation was delivered to five discrete locations on the sole of the foot (heel, and medial and lateral sites on the midfoot and forefoot) during treadmill walking. EMG activity from muscles acting at the hip, knee and ankle were recorded along with movement at these three joints. Additionally, 3 force sensing resistors measuring continuous force changes were placed at the heel, and the medial and lateral aspects of the right foot sole. All data were sorted based on stimulus occurrence in twelve step-cycle phases, before being averaged together within a phase for subsequent analysis. Results The results demonstrate statistically significant dynamic changes in reflex amplitudes, kinematics and foot sole pressures that are site-specific and phase-dependent. The general trends demonstrate responses producing decreased underfoot pressure at the site of stimulation. Conclusions The responses to stimulation of discrete locations on the foot sole evoke a kind of “sensory steering” that may promote balance and maintenance of locomotion through the modulation of limb loading and foot placement. These results have implications for using sensory stimulation as a therapeutic modality during gait retraining (e.g. after stroke) as well as for footwear design and implementation of foot sole contact surfaces during gait. PMID:25202452

IN-SITU THERMAL TREATMENT SYSTEM PERFORMANCE AND MASS REMOVAL METRICS AT FORT LEWIS

EPA Science Inventory

The EGDY is the source of a potentially expanding three mile long TCE plume in a sole source drinking water aquifer. Thermal remediation is being employed to reduce source mass loading to the dissolved phase aquifer plume and reduce the time to reach site cleanup goals. This is...

LESSONS LEARNED FROM IN-SITU RESISTIVE HEATING OF TCE AT FORT LEWIS, WASHINGTON

EPA Science Inventory

The EGDY is the source of a potentially expanding, three mile long TCE plume in a sole source drinking water aquifer. Thermal remediation is being employed to reduce source mass loading to the dissolved phase aquifer plume and reduce the time to reach site cleanup goals. This i...

Utilization of Dimethyl Sulfide as a Sulfur Source with the Aid of Light by Marinobacterium sp. Strain DMS-S1

PubMed Central

Fuse, Hiroyuki; Takimura, Osamu; Murakami, Katsuji; Yamaoka, Yukiho; Omori, Toshio

2000-01-01

Strain DMS-S1 isolated from seawater was able to utilize dimethyl sulfide (DMS) as a sulfur source only in the presence of light in a sulfur-lacking medium. Phylogenetic analysis based on 16S ribosomal DNA genes indicated that the strain was closely related to Marinobacterium georgiense. The strain produced dimethyl sulfoxide (DMSO), which was a main metabolite, and small amounts of formate and formaldehyde when grown on DMS as the sole sulfur source. The cells of the strain grown with succinate as a carbon source were able to use methyl mercaptan or methanesulfonate besides DMS but not DMSO or dimethyl sulfone as a sole sulfur source. DMS was transformed to DMSO primarily at wavelengths between 380 and 480 nm by heat-stable photosensitizers released by the strain. DMS was also degraded to formaldehyde in the presence of light by unidentified heat-stable factors released by the strain, and it appeared that strain DMS-S1 used the degradation products, which should be sulfite, sulfate, or methanesulfonate, as sulfur sources. PMID:11097944

Growth of Pseudomonas C on C1 Compounds: Continuous Culture

PubMed Central

Battat, E.; Goldberg, I.; Mateles, R. I.

1974-01-01

Pseudomonas C was grown in continuous culture on methanol, formaldehyde, or formate as sole carbon source. On methanol μmax = 0.49/h and yield constant (Y) = 0.54; on formaldehyde and on unsupplemented media, μmax was about 0.2/h and Y was 0.15, whereas addition of p-aminobenzoic acid, folic acid, serine, or glycine to the medium raised Y to about 0.26 to 0.29, and addition of p-aminobenzoic acid, folic acid, serine, nicotinamide adenine dinucleotide, and Tween 80 raised the yield to 0.35. On formate and on unsupplemented media, μmax = 0.2/h and Y = 0.02, whereas addition of 0.1 mM p-aminobenzoic acid increased μmax to about 0.47 and Y to about 0.23. At low cell concentrations or growth rates a beneficial effect of CO2 was observed. Formaldehyde or formate, when added together with methanol, were utilized simultaneously with the methanol. PMID:4375436

Production of polyhydroxybutyrate by the marine photosynthetic bacterium Rhodovulum sulfidophilum P5

NASA Astrophysics Data System (ADS)

Cai, Jinling; Wei, Ying; Zhao, Yupeng; Pan, Guanghua; Wang, Guangce

2012-07-01

The effects of different NaCl concentrations, nitrogen sources, carbon sources, and carbon to nitrogen molar ratios on biomass accumulation and polyhydroxybutyrate (PHB) production were studied in batch cultures of the marine photosynthetic bacterium Rhodovulum sulfidophilum P5 under aerobic-dark conditions. The results show that the accumulation of PHB in strain P5 is a growth-associated process. Strain P5 had maximum biomass and PHB accumulation at 2%-3% NaCl, suggesting that the bacterium can maintain growth and potentially produce PHB at natural seawater salinity. In the nitrogen source test, the maximum biomass accumulation (8.10±0.09 g/L) and PHB production (1.11±0.13 g/L and 14.62%±2.2 of the cell dry weight) were observed when peptone and ammonium chloride were used as the sole nitrogen source. NH{4/+}-N was better for PHB production than other nitrogen sources. In the carbon source test, the maximum biomass concentration (7.65±0.05 g/L) was obtained with malic acid as the sole carbon source, whereas the maximum yield of PHB (5.03±0.18 g/L and 66.93%±1.69% of the cell dry weight) was obtained with sodium pyruvate as the sole carbon source. In the carbon to nitrogen ratios test, sodium pyruvate and ammonium chloride were selected as the carbon and nitrogen sources, respectively. The best carbon to nitrogen molar ratio for biomass accumulation (8.77±0.58 g/L) and PHB production (6.07±0.25 g/L and 69.25%±2.05% of the cell dry weight) was 25. The results provide valuable data on the production of PHB by R. sulfidophilum P5 and further studies are on-going for best cell growth and PHB yield.

Metamorphic sole formation reveals plate interface rheology during early subduction

NASA Astrophysics Data System (ADS)

Mathieu, S.; Agard, P.; Dubacq, B.; Plunder, A.; Prigent, C.

2015-12-01

Metamorphic soles are m to ~500m thick tectonic slices welded beneath most large ophiolites. They correspond to highly to mildly deformed portions of oceanic lithosphere metamorphosed at amphibolite to granulite facies peak conditions. Metamorphic soles are interpreted as formed ≤1-2Ma after intraoceanic subduction initiation by heat transfer from the hot, incipient mantle wegde to the underthrusting lower plate. Their early accretion and exhumation together with the future ophiolite implies at least one jump of the subduction plate interface from above to below the metamorphic sole. Metamorphic soles thus represent one of the few remnants of the very early evolution of the subduction plate interface and provide major constraints on the thermal structure and the effective rheology of the crust and mantle along the nascent slab interface.We herein present a structural and petrological detailed description of the Oman and Turkey metamorphic soles. Both soles present a steep inverted metamorphic structure, with isograds subparallel to the peridotite contact, in which the proportion of mafic rocks, pressure and temperature conditions increase upward. They comprise, as most metamorphic soles worldwide, two main units: (1) a high-grade unit adjacent to the overlying peridotite composed of granulitized to amphibolized metabasalts, with rare metasedimentary interlayers (~800±100ºC at 10±2kbar) and (2) a low-grade greenschist facies unit composed of metasedimentary rocks with rare metatuffs (~500±100ºC at 5±2kbar). We provide for the first time refined P-T peak condition estimations by means of pseudosection modelling and maximum temperature constraints for the Oman low-grade sole by RAMAN thermometry. In order to quantify micro-scale deformations trough the sole, we also present EBSD data on the Oman garnet-bearing and garnet-free high-grade sole.With these new constraints, we finally propose a new conceptual mechanical model for metamorphic sole formation. This model excludes the presence of a continuous inverted metamorphic gradient through the sole but implies the stacking of several homogeneous slivers to constitute the present structure of the sole. These successive thrusts are the result of rheological changes as the plate interface progressively cools.

48 CFR 15.306 - Exchanges with offerors after receipt of proposals.

Code of Federal Regulations, 2011 CFR

2011-10-01

... negotiations may include bargaining. Bargaining includes persuasion, alteration of assumptions and positions... ACQUISITION REGULATION CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Source Selection 15.... Negotiations are exchanges, in either a competitive or sole source environment, between the Government and...

48 CFR 15.306 - Exchanges with offerors after receipt of proposals.

Code of Federal Regulations, 2010 CFR

2010-10-01

... negotiations may include bargaining. Bargaining includes persuasion, alteration of assumptions and positions... ACQUISITION REGULATION CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Source Selection 15.... Negotiations are exchanges, in either a competitive or sole source environment, between the Government and...

48 CFR 2901.603-70 - Responsibility of other government personnel.

Code of Federal Regulations, 2011 CFR

2011-10-01

... and open competition are satisfied to the maximum extent practicable. Sole source purchases may only... LABOR GENERAL DEPARTMENT OF LABOR ACQUISITION REGULATION SYSTEM Career Development, Contracting... being overly restrictive in accordance with FAR 11.002; (2) Competitive sources are solicited, evaluated...

Insight into subdecimeter fracturing processes during hydraulic fracture experiment in Äspö hard rock laboratory, Sweden

NASA Astrophysics Data System (ADS)

Kwiatek, Grzegorz; Martínez-Garzón, Patricia; Plenkers, Katrin; Leonhardt, Maria; Zang, Arno; Dresen, Georg; Bohnhoff, Marco

2017-04-01

We analyze the nano- and picoseismicity recorded during a hydraulic fracturing in-situ experiment performed in Äspö Hard Rock Laboratory, Sweden. The fracturing experiment included six fracture stages driven by three different water injection schemes (continuous, progressive and pulse pressurization) and was performed inside a 28 m long, horizontal borehole located at 410 m depth. The fracturing process was monitored with two different seismic networks covering a wide frequency band between 0.01 Hz and 100000 Hz and included broadband seismometers, geophones, high-frequency accelerometers and acoustic emission sensors. The combined seismic network allowed for detection and detailed analysis of seismicity with moment magnitudes MW<-4 (source sizes approx. on cm scale) that occurred solely during the hydraulic fracturing and refracturing stages. We relocated the seismicity catalog using the double-difference technique and calculated the source parameters (seismic moment, source size, stress drop, focal mechanism and seismic moment tensors). The physical characteristics of induced seismicity are compared to the stimulation parameters and to the formation parameters of the site. The seismic activity varies significantly depending on stimulation strategy with conventional, continuous stimulation being the most seismogenic. We find a systematic spatio-temporal migration of microseismic events (propagation away and towards wellbore injection interval) and temporal transitions in source mechanisms (opening - shearing - collapse) both being controlled by changes in fluid injection pressure. The derived focal mechanism parameters are in accordance with the local stress field orientation, and signify the reactivation of pre-existing rock flaws. The seismicity follows statistical and source scaling relations observed at different scales elsewhere, however, at an extremely low level of seismic efficiency.

29 CFR 2590.731 - Preemption; State flexibility; construction.

Code of Federal Regulations, 2010 CFR

2010-07-01

... continues in effect any standard or requirement solely relating to health insurance issuers in connection with group health insurance coverage except to the extent that such standard or requirement prevents... establishes, implements, or continues in effect a standard or requirement applicable to imposition of a...

32 CFR 317.4 - Responsibilities.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) PRIVACY PROGRAM DCAA PRIVACY ACT PROGRAM § 317.4 Responsibilities. (a) The Assistant Director, Resources has overall responsibility for the DCAA Privacy Act Program and will serve as the sole appellate authority for appeals to...

Mineralization of the Cyclic Nitramine Explosive Hexahydro-1,3,5-Trinitro-1,3,5-Triazine by Gordonia and Williamsia spp.

PubMed Central

Thompson, Karen T.; Crocker, Fiona H.; Fredrickson, Herbert L.

2005-01-01

Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) is a cyclic nitroamine explosive that is a major component in many military high-explosive formulations. In this study, two aerobic bacteria that are capable of using RDX as the sole source of carbon and nitrogen to support their growth were isolated from surface soil. These bacterial strains were identified by their fatty acid profiles and 16S ribosomal gene sequences as Williamsia sp. KTR4 and Gordonia sp. KTR9. The physiology of each strain was characterized with respect to the rates of RDX degradation and [U-14C]RDX mineralization when RDX was supplied as a sole carbon and nitrogen source in the presence and absence of competing carbon and nitrogen sources. Strains KTR4 and KTR9 degraded 180 μM RDX within 72 h when RDX served as the only added carbon and nitrogen source while growing to total protein concentrations of 18.6 and 16.5 μg/ml, respectively. Mineralization of [U-14C]RDX to 14CO2 was 30% by strain KTR4 and 27% by KTR9 when RDX was the only added source of carbon and nitrogen. The addition of (NH4)2SO4 greatly inhibited KTR9's degradation of RDX but had little effect on that of KTR4. These are the first two pure bacterial cultures isolated that are able to use RDX as a sole carbon and nitrogen source. These two genera possess different physiologies with respect to RDX mineralization, and each can serve as a useful microbiological model for the study of RDX biodegradation with regard to physiology, biochemistry, and genetics. PMID:16332812

Conidiation of Penicillium camemberti in submerged liquid cultures is dependent on the nitrogen source.

PubMed

Boualem, Khadidja; Labrie, Steve; Gervais, Patrick; Waché, Yves; Cavin, Jean-François

2016-02-01

To study the ability of a commercial Penicillium camemberti strain, used for Camembert type cheese ripening, to produce conidia during growth in liquid culture (LC), in media containing different sources of nitrogen as, industrially, conidia are produced by growth at the surface of a solid state culture because conidiation in stirred submerged aerobic LC is not known. In complex media containing peptic digest of meat, hyphae ends did not differentiate into phialides and conidia. Contrarily, in a synthetic media containing KNO3 as sole nitrogen source, hyphae ends differentiated into phialides producing 0.5 × 10(7) conidia/ml. Conidia produced in LC were 25 % less hydrophobic than conidia produced in solid culture, and this correlates with a seven-times-lower expression of the gene rodA encoding hydrophobin RodA in the mycelium grown in LC. Conidiation of P. camembertii is stimulated in iquid medium containing KNO3 as sole source of nitrogen and therefore opens up opportunities for using liquid medium in commercial productions.

S5 Lipase: an organic solvent tolerant enzyme.

PubMed

Rahman, Raja Noor Zaliha Abdul; Baharum, Syarul Nataqain; Salleh, Abu Bakar; Basri, Mahiran

2006-12-01

In this study, an organic solvent tolerant bacterial strain was isolated. This strain was identified as Pseudomonas sp. strain S5, and was shown to degrade BTEX (Benzene, Toluene, Ethyl-Benzene, and Xylene). Strain S5 generates an organic solvent-tolerant lipase in the late logarithmic phase of growth. Maximum lipase production was exhibited when peptone was utilized as the sole nitrogen source. Addition of any of the selected carbon sources to the medium resulted in a significant reduction of enzyme production. Lower lipase generation was noted when an inorganic nitrogen source was used as the sole nitrogen source. This bacterium hydrolyzed all tested triglycerides and the highest levels of production were observed when olive oil was used as a natural triglyceride. Basal medium containing Tween 60 enhanced lipase production to the most significant degree. The absence of magnesium ions (Mg2+) in the basal medium was also shown to stimulate lipase production. Meanwhile, an alkaline earth metal ion, Na+, was found to stimulate the production of S5 lipase.

Methanol Expression Regulator 1 (Mxr1p) Is Essential for the Utilization of Amino Acids as the Sole Source of Carbon by the Methylotrophic Yeast, Pichia pastoris.

PubMed

Sahu, Umakant; Rangarajan, Pundi N

2016-09-23

Unlike Saccharomyces cerevisiae, the methylotrophic yeast Pichia pastoris can assimilate amino acids as the sole source of carbon and nitrogen. It can grow in media containing yeast extract and peptone (YP), yeast nitrogen base (YNB) + glutamate (YNB + Glu), or YNB + aspartate (YNB + Asp). Methanol expression regulator 1 (Mxr1p), a zinc finger transcription factor, is essential for growth in these media. Mxr1p regulates the expression of several genes involved in the utilization of amino acids as the sole source of carbon and nitrogen. These include the following: (i) GDH2 encoding NAD-dependent glutamate dehydrogenase; (ii) AAT1 and AAT2 encoding mitochondrial and cytosolic aspartate aminotransferases, respectively; (iii) MDH1 and MDH2 encoding mitochondrial and cytosolic malate dehydrogenases, respectively; and (iv) GLN1 encoding glutamine synthetase. Synthesis of all these enzymes is regulated by Mxr1p at the level of transcription except GDH2, whose synthesis is regulated at the level of translation. Mxr1p activates the transcription of AAT1, AAT2, and GLN1 in cells cultured in YP as well as in YNB + Glu media, whereas transcription of MDH1 and MDH2 is activated in cells cultured in YNB + Glu but not in YP. A truncated Mxr1p composed of 400 N-terminal amino acids activates transcription of target genes in cells cultured in YP but not in YNB + Glu. Mxr1p binds to Mxr1p response elements present in the promoters of AAT2, MDH2, and GLN1 We conclude that Mxr1p is essential for utilization of amino acids as the sole source of carbon and nitrogen, and it is a global regulator of multiple metabolic pathways in P. pastoris. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

Oman metamorphic sole formation reveals early subduction dynamics

NASA Astrophysics Data System (ADS)

Soret, Mathieu; Agard, Philippe; Dubacq, Benoît; Plunder, Alexis; Ildefonse, Benoît; Yamato, Philippe; Prigent, Cécile

2016-04-01

Metamorphic soles correspond to m to ~500m thick tectonic slices welded beneath most of the large-scale ophiolites. They typically show a steep inverted metamorphic structure where the pressure and temperature conditions of crystallization increase upward (from 500±100°C at 0.5±0.2 GPa to 800±100°C at 1.0±0.2 GPa), with isograds subparallel to the contact with the overlying ophiolitic peridotite. The proportion of mafic rocks in metamorphic soles also increases from the bottom (meta-sediments rich) to the top (approaching the ophiolite peridotites). These soles are interpreted as the result of heat transfer from the incipient mantle wedge toward the nascent slab (associated with large-scale fluid transfer and possible shear heating) during the first My of intra-oceanic subduction (as indicated by radiometric ages). Metamorphic soles provide therefore major constraints on early subduction dynamics (i.e., thermal structure, fluid migration and rheology along the nascent slab interface). We present a detailed structural and petrological study of the metamorphic sole from 4 major cross-sections along the Oman ophiolite. We show precise pressure-temperature estimates obtained by pseudosection modelling and EBSD measurements performed on both the garnet-bearing and garnet-free high-grade sole. Results allow quantification of the micro-scale deformation and highlight differences in pressure-temperature-deformation conditions between the 4 different locations, showing that the inverted metamorphic gradient through the sole is not continuous in all locations. Based on these new constraints, we suggest a new tectonic-petrological model for the formation of metamorphic soles below ophiolites. This model involves the stacking of several homogeneous slivers of oceanic crust leading to the present-day structure of the sole. In this view, these thrusts are the result of rheological contrasts between the sole and the peridotite as the plate interface progressively cools down. These slivers later underwent several stages of retrogression (partly mediated by ascending fluids from the slab) from amphibolite- to prehnite/pumpellite-facies conditions.

Carbon utilization profiles of river bacterial strains facing sole carbon sources suggest metabolic interactions.

PubMed

Goetghebuer, Lise; Servais, Pierre; George, Isabelle F

2017-05-01

Microbial communities play a key role in water self-purification. They are primary drivers of biogenic element cycles and ecosystem processes. However, these communities remain largely uncharacterized. In order to understand the diversity-heterotrophic activity relationship facing sole carbon sources, we assembled a synthetic community composed of 20 'typical' freshwater bacterial species mainly isolated from the Zenne River (Belgium). The carbon source utilization profiles of each individual strain and of the mixed community were measured in Biolog Phenotype MicroArrays PM1 and PM2A microplates that allowed testing 190 different carbon sources. Our results strongly suggest interactions occurring between our planktonic strains as our synthetic community showed metabolic properties that were not displayed by its single components. Finally, the catabolic performances of the synthetic community and a natural community from the same sampling site were compared. The synthetic community behaved like the natural one and was therefore representative of the latter in regard to carbon source consumption. © FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Large Plasmids from Soil Bacteria Enriched on Halogenated Alkanoic Acids

PubMed Central

Hardman, David J.; Gowland, Peter C.; Slater, J. Howard

1986-01-01

Four Pseudomonas species and two Alcaligenes species were isolated from soil with a capacity to grow on halogenated alkanoic acids. They were shown to contain one of five large plasmids. The plasmids had molecular weights ranging from 98,800 to 190,000. They were associated with the ability to utilize the halogenated substrates 2-monochloropropionic acid and 2-monochloroacetic acid and with resistance towards one or more of the heavy metals mercury, selenium, and tellurium. The largest plasmid, pUU204, was shown to be unstable in continuous-flow culture when the organism was supplied with succinate as the sole carbon source. The dehalogenase gene associated with pUU204 appeared to be readily transferred to an incP group plasmid, R68-45. PMID:16346975

Aerobic Degradation of 2,4,6-Trinitrotoluene by Enterobacter cloacae PB2 and by Pentaerythritol Tetranitrate Reductase

PubMed Central

French, Christopher E.; Nicklin, Stephen; Bruce, Neil C.

1998-01-01

Enterobacter cloacae PB2 was originally isolated on the basis of its ability to utilize nitrate esters, such as pentaerythritol tetranitrate (PETN) and glycerol trinitrate, as the sole nitrogen source for growth. The enzyme responsible is an NADPH-dependent reductase designated PETN reductase. E. cloacae PB2 was found to be capable of slow aerobic growth with 2,4,6-trinitrotoluene (TNT) as the sole nitrogen source. Dinitrotoluenes were not produced and could not be used as nitrogen sources. Purified PETN reductase was found to reduce TNT to its hydride-Meisenheimer complex, which was further reduced to the dihydride-Meisenheimer complex. Purified PETN reductase and recombinant Escherichia coli expressing PETN reductase were able to liberate nitrogen as nitrite from TNT. The ability to remove nitrogen from TNT suggests that PB2 or recombinant organisms expressing PETN reductase may be useful for bioremediation of TNT-contaminated soil and water. PMID:9687442

Microbial recycling of glycerol to biodiesel.

PubMed

Yang, Liu; Zhu, Zhi; Wang, Weihua; Lu, Xuefeng

2013-12-01

The sustainable supply of lipids is the bottleneck for current biodiesel production. Here microbial recycling of glycerol, byproduct of biodiesel production to biodiesel in engineered Escherichia coli strains was reported. The KC3 strain with capability of producing fatty acid ethyl esters (FAEEs) from glucose was used as a starting strain to optimize fermentation conditions when using glycerol as sole carbon source. The YL15 strain overexpressing double copies of atfA gene displayed 1.7-fold increase of FAEE productivity compared to the KC3 strain. The titer of FAEE in YL15 strain reached to 813 mg L(-1) in minimum medium using glycerol as sole carbon source under optimized fermentation conditions. The titer of glycerol-based FAEE production can be significantly increased by both genetic modifications and fermentation optimization. Microbial recycling of glycerol to biodiesel expands carbon sources for biodiesel production. Copyright © 2013 Elsevier Ltd. All rights reserved.

Production of ligninolytic enzymes and some diffusible antifungal compounds by white-rot fungi using potato solid wastes as the sole nutrient source.

PubMed

Schalchli, H; Hormazábal, E; Rubilar, O; Briceño, G; Mutis, A; Zocolo, G J; Diez, M C

2017-10-01

The aim of this study was to evaluate the synthesis of ligninolytic enzymes and some diffusible antifungal compounds by white-rot fungi (WRF) using peels or discarded potato as the sole nutrient source. The strain Trametes hirsuta Ru-513 highlighted for its laccase activity (595 ± 33 U l -1 ), which is able to decolourize 87% of an anthraquinone dye using potato peels as the sole nutritional support. A native polyacrylamide gel of laccase proteins showed the presence of two isoenzymes, corresponding to proteins of 56 and 67 kDa, which were detected by SDS-PAGE. The antifungal activity of ethyl acetate extracts was evaluated by the agar diffusion method, where Anthracophyllum discolor Sp4 and Inonotus sp. Sp2 showed the highest inhibition zones of Mucor miehei. The fungal extracts also inhibited Fusarium oxysporum and Botrytis cinerea growth, with inhibition zones of up to 18 mm. The extract with the highest antifungal activity, from A. discolor Sp4 grown in discarded potato medium, was analysed using a gas chromatograph coupled to a mass spectrometer. Among the identified compounds, chlorinated aromatic compounds and veratryl alcohol were the most abundant compounds. The results revealed the relevance of potato waste valorization for the sustainable production of ligninolytic enzymes and antifungal compounds. This study reports the synthesis of ligninolytic enzymes and diffusible antifungal compounds by WRF using potato wastes as the sole nutrient source and suggests a relationship between the enzymatic activity and the synthesis of antifungal compounds. These compounds and the synthesis of halogen compounds by WRF using agro-industrial wastes have been poorly studied before. © 2017 The Society for Applied Microbiology.

40 CFR 144.86 - What are the definitions I need to know?

Code of Federal Regulations, 2014 CFR

2014-07-01

... of ground water protection areas are identified. (g) Other sensitive ground water areas. States may... to protecting underground sources of drinking water from contamination. These other sensitive ground water areas may include areas such as areas overlying sole-source aquifers; highly productive aquifers...

40 CFR 144.86 - What are the definitions I need to know?

Code of Federal Regulations, 2011 CFR

2011-07-01

... of ground water protection areas are identified. (g) Other sensitive ground water areas. States may... to protecting underground sources of drinking water from contamination. These other sensitive ground water areas may include areas such as areas overlying sole-source aquifers; highly productive aquifers...

40 CFR 144.86 - What are the definitions I need to know?

Code of Federal Regulations, 2012 CFR

2012-07-01

... of ground water protection areas are identified. (g) Other sensitive ground water areas. States may... to protecting underground sources of drinking water from contamination. These other sensitive ground water areas may include areas such as areas overlying sole-source aquifers; highly productive aquifers...

40 CFR 144.86 - What are the definitions I need to know?

Code of Federal Regulations, 2013 CFR

2013-07-01

... of ground water protection areas are identified. (g) Other sensitive ground water areas. States may... to protecting underground sources of drinking water from contamination. These other sensitive ground water areas may include areas such as areas overlying sole-source aquifers; highly productive aquifers...

24 CFR 291.565 - Continuing obligations after purchase.

Code of Federal Regulations, 2010 CFR

2010-04-01

... purchase. 291.565 Section 291.565 Housing and Urban Development Regulations Relating to Housing and Urban... Neighbor Next Door Sales Program § 291.565 Continuing obligations after purchase. To remain in compliance.../her sole residence, the home purchased through the GNND Sales Program; and (b) Certify initially and...

Case study: molasses as the primary energy source on an organic grazing dairy

USDA-ARS?s Scientific Manuscript database

Organic dairies face many challenges, one of which is the high cost of purchased organic grains. Molasses may be a less expensive energy alternative. However, anecdotal results have been mixed for farms that used molasses as the sole energy source. This research project quantified animal performance...

Evaluation of a Sustainable Remediation Option: Beneficial Reuse of Petroleum-Contaminated Sediment as an Energy Source

EPA Science Inventory

The characteristics of petroleum-contaminated sediment (PCS) have been evaluated to assess whether the practice of its beneficial reuse as a sole or supplemental energy source is sustainable relative to other sediment remediation options such as monitored natural recovery (MNR), ...

40 CFR 256.50 - Requirements.

Code of Federal Regulations, 2010 CFR

2010-07-01

... underground sources of drinking water under the authority of section 1442(a)(8)(C) of the Safe Drinking Water... Drinking Water Act (42 U.S.C. 300f et seq.) and with the designation of sole source aquifers under section... recovery and solid waste disposal programs at various installations); (8) Department of Energy (State...

40 CFR 256.50 - Requirements.

Code of Federal Regulations, 2012 CFR

2012-07-01

... underground sources of drinking water under the authority of section 1442(a)(8)(C) of the Safe Drinking Water... Drinking Water Act (42 U.S.C. 300f et seq.) and with the designation of sole source aquifers under section... recovery and solid waste disposal programs at various installations); (8) Department of Energy (State...

40 CFR 256.50 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-07-01

... underground sources of drinking water under the authority of section 1442(a)(8)(C) of the Safe Drinking Water... Drinking Water Act (42 U.S.C. 300f et seq.) and with the designation of sole source aquifers under section... recovery and solid waste disposal programs at various installations); (8) Department of Energy (State...

40 CFR 256.50 - Requirements.

Code of Federal Regulations, 2011 CFR

2011-07-01

... underground sources of drinking water under the authority of section 1442(a)(8)(C) of the Safe Drinking Water... Drinking Water Act (42 U.S.C. 300f et seq.) and with the designation of sole source aquifers under section... recovery and solid waste disposal programs at various installations); (8) Department of Energy (State...

40 CFR 256.50 - Requirements.

Code of Federal Regulations, 2013 CFR

2013-07-01

... underground sources of drinking water under the authority of section 1442(a)(8)(C) of the Safe Drinking Water... Drinking Water Act (42 U.S.C. 300f et seq.) and with the designation of sole source aquifers under section... recovery and solid waste disposal programs at various installations); (8) Department of Energy (State...

48 CFR 1306.302-5 - Authorized or required by statute.

Code of Federal Regulations, 2010 CFR

2010-10-01

... COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Other Than Full and Open Competition 1306.302-5 Authorized or required by statute. In accordance with Executive Order 13457, a sole source acquisition may not be justified on the basis of any earmark included in any non-statutory source, except when...

BOOK REVIEW OF "ASSESSMENT AND CONTROL OF NONPOINT SOURCE POLLUTION OF AQUATIC ECOSYSTEMS: A PRACTICAL APPROACH"

EPA Science Inventory

This book is geared to environmental specialists and planners, heavy on the technical side. It goes beyond tranditional nonpoint source (NPS) approaches which typically only look at stormwater as athe sole NPS pollution driver. There is some overreaching material beyond the conte...

Multiple Sources of Prescription Payment and Risky Opioid Therapy Among Veterans.

PubMed

Becker, William C; Fenton, Brenda T; Brandt, Cynthia A; Doyle, Erin L; Francis, Joseph; Goulet, Joseph L; Moore, Brent A; Torrise, Virginia; Kerns, Robert D; Kreiner, Peter W

2017-07-01

Opioid overdose and other related harms are a major source of morbidity and mortality among US Veterans, in part due to high-risk opioid prescribing. We sought to determine whether having multiple sources of payment for opioids-as a marker for out-of-system access-is associated with risky opioid therapy among veterans. Cross-sectional study examining the association between multiple sources of payment and risky opioid therapy among all individuals with Veterans Health Administration (VHA) payment for opioid analgesic prescriptions in Kentucky during fiscal year 2014-2015. Source of payment categories: (1) VHA only source of payment (sole source); (2) sources of payment were VHA and at least 1 cash payment [VHA+cash payment(s)] whether or not there was a third source of payment; and (3) at least one other noncash source: Medicare, Medicaid, or private insurance [VHA+noncash source(s)]. Our outcomes were 2 risky opioid therapies: combination opioid/benzodiazepine therapy and high-dose opioid therapy, defined as morphine equivalent daily dose ≥90 mg. Of the 14,795 individuals in the analytic sample, there were 81.9% in the sole source category, 6.6% in the VHA+cash payment(s) category, and 11.5% in the VHA+noncash source(s) category. In logistic regression, controlling for age and sex, persons with multiple payment sources had significantly higher odds of each risky opioid therapy, with those in the VHA+cash having significantly higher odds than those in the VHA+noncash source(s) group. Prescribers should examine the prescription monitoring program as multiple payment sources increase the odds of risky opioid therapy.

Future groundwater extraction scenarios for an aquifer in a semiarid environment: case study of Guadalupe Valley Aquifer, Baja California, Northwest Mexico.

PubMed

Campos-Gaytan, Jose Ruben; Kretzschmar, Thomas; Herrera-Oliva, Claudia Soledad

2014-11-01

Semiarid northwestern Mexico presents a growing water demand produced by agricultural and domestic requirements during the last two decades. The community of Guadalupe Valley and the city of Ensenada rely on groundwater pumping from the local aquifer as its sole source of water supply. This dependency has resulted in an imbalance between groundwater pumpage and natural recharge. A two-dimensional groundwater flow model was applied to the Guadalupe Valley Aquifer, which was calibrated and validated for the period 1984-2005. The model analysis verified that groundwater levels in the region are subject to steep declines due to decades of intensive groundwater exploitation for agricultural and domestic purposes. The calibrated model was used to assess the effects of different water management scenarios for the period 2007-2025. If the base case (status quo) scenario continues, groundwater levels are in a continuous drawdown trend. Some wells would run dry by August 2017, and water demand may not be met without incurring in an overdraft. The optimistic scenario implies the achievement of the mean groundwater recharge and discharge. Groundwater level depletion could be stopped and restored. The sustainable scenario implies the reduction of current extraction (up to about 50 %), when groundwater level depletion could be stopped. A reduction in current extraction mitigates water stress in the aquifer but cannot solely reverse declining water tables across the region. The combination of reduced current extraction and an implemented alternative solution (such as groundwater artificial recharge), provides the most effective measure to stabilize and reverse declining groundwater levels while meeting water demands in the region.

Degradation and mineralization of atrazine by a soil bacterial isolate.

PubMed Central

Radosevich, M; Traina, S J; Hao, Y L; Tuovinen, O H

1995-01-01

An atrazine-degrading bacterial culture was isolated from an agricultural soil previously impacted by herbicide spills. The organism was capable of using atrazine under aerobic conditions as the sole source of C and N. Cyanuric acid could replace atrazine as the sole source of N, indicating that the organism was capable of ring cleavage. Ring cleavage was confirmed in 14CO2 evolution experiments with [U-14C-ring]atrazine. Between 40 and 50% of ring-14C was mineralized to 14CO2. [14C]biuret and [14C]urea were detected in spent culture media. Cellular assimilation of 14C was negligible, in keeping with the fully oxidized valence of the ring carbon. Chloride release was stoichiometric. The formation of ammonium during atrazine degradation was below the stoichiometric amount, suggesting a deficit due to cellular assimilation and metabolite-N accumulation. With excess glucose and with atrazine as the sole N source, free ammonium was not detected, suggesting assimilation into biomass. The organism degraded atrazine anaerobically in media which contained (i) atrazine only, (ii) atrazine and glucose, and (iii) atrazine, glucose, and nitrate. To date, this is the first report of a pure bacterial isolate with the ability to cleave the s-triazine ring structure of atrazine. It was also concluded that this bacterium was capable of dealkylation, dechlorination, and deamination in addition to ring cleavage. PMID:7887609

Science for the stewardship of the groundwater resources of Cape Cod, Massachusetts

USGS Publications Warehouse

Barbaro, Jeffrey R.; Masterson, John P.; LeBlanc, Denis R.

2014-01-01

Groundwater is the sole source of drinking water and a major source of freshwater for domestic, industrial, and agricultural uses on Cape Cod, Massachusetts. Groundwater discharged from aquifers also supports freshwater pond and stream ecosystems and coastal wetlands. Six hydraulically distinct groundwater-flow systems (lenses) have been delineated on Cape Cod. Of the approximately 450 million gallons per day of water that enters these lenses as recharge from precipitation, about 69 percent discharges directly to the coast, about 24 percent discharges to streams, and almost 7 percent is withdrawn by public-supply wells. In most areas, groundwater in the sand and gravel aquifers is shallow and susceptible to contamination from anthropogenic sources and saltwater intrusion. Continued land development and population growth on Cape Cod have created concerns that potable water will become less available and that the quantity and quality of water flowing to natural discharge areas such as ponds, streams, and coastal waters will continue to decline. The U.S. Geological Survey (USGS) has been investigating groundwater and surface-water resources on Cape Cod for more than 50 years. Recent studies highlighted in this fact sheet have focused on the sources of water to public-supply wells, ponds, streams, and coastal areas; the transport and discharge of nitrogen derived from domestic and municipal disposal of wastewater; and the effects of climate change on groundwater and surface-water resources. Other USGS activities include long-term monitoring of groundwater and pond levels and field research on groundwater contamination at the USGS Cape Cod Toxic Substances Hydrology Research Site (http://ma.water.usgs.gov/MMRCape/) near the Joint Base Cape Cod (JBCC), formerly the Massachusetts Military Reservation.

26 CFR 1.312-4 - Examples of adjustments provided in section 312(c).

Code of Federal Regulations, 2010 CFR

2010-04-01

... 26 Internal Revenue 4 2010-04-01 2010-04-01 false Examples of adjustments provided in section 312... (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES Effects on Corporation § 1.312-4 Examples of adjustments... examples: Example 1. On December 2, 1954, Corporation X distributed to its sole shareholder, A, an...

48 CFR 1503.670 - Solicitation of disclosure provision.

Code of Federal Regulations, 2010 CFR

2010-10-01

... GENERAL IMPROPER BUSINESS PRACTICES AND PERSONAL CONFLICTS OF INTEREST Contracts with Government Employees... Certification, in all solicitations for sole source acquisitions. [50 FR 14357, Apr. 11, 1985] ...

The effect of spiked boots on logger safety, productivity and workload.

PubMed

Kirk, P; Parker, R

1994-04-01

Analysis of 1657 lost-time logging accidents in the New Zealand logging industry (1985-1991) indicates that 17.5% were as a result of slips, trips and falls and a total of 2870 days were lost. Most (56%) of these slipping, tripping and falling accidents occurred in the felling and delimbing phase of the logging operation, where 37% of the workforce are employed. In an attempt to reduce the number of slipping injuries to loggers employed in felling and delimbing, a study of the effectiveness of spike-soled (caulk) boots was undertaken. Four loggers were intensively observed at work, by continuous time-study methods, while wearing their conventional rubber-soled boots and then spike-soled boots. The number of slips, work methods used, physiological workload and productivity were compared for loggers wearing the two footwear types. Results indicated that spike-soled boots were associated with a significant reduction in the frequency of slips and had no adverse effect on work methods, physiological workload or productivity. Spike-soled boots are now being promoted for use by loggers in New Zealand as a simple method to reduce slipping, tripping and falling accidents.

Influence of electron donors and copper concentration on geochemical and mineralogical processes under conditions of biological sulphate reduction

NASA Astrophysics Data System (ADS)

Wolicka, Dorota; Borkowski, Andrzej

2014-03-01

Sulphidogenous microorganism communities were isolated from soil polluted by crude oil. The study was focused on determining the influence of 1) copper (II) concentration on the activity of selected microorganism communities and 2) the applied electron donor on the course and evolution of mineral-forming processes under conditions favouring growth of sulphate-reducing bacteria (SRB). The influence of copper concentration on the activity of selected microorganism communities and the type of mineral phases formed was determined during experiments in which copper (II) chloride at concentrations of 0.1, 0.2, 0.5 and 0.7 g/L was added to SRB cultures. The experiments were performed in two variants: with ethanol (4 g/L) or lactate (4 g/L) as the sole carbon source. In order to determine the taxonomic composition of the selected microorganism communities, the 16S rRNA method was used. Results of this analysis confirmed the presence of Desulfovibrio, Desulfohalobium, Desulfotalea, Thermotoga, Solibacter, Gramella, Anaeromyxobacter and Myxococcus sp. in the stationary cultures. The post-culture sediments contained covelline (CuS) and digenite (Cu9S5 ). Based on the results, it can be stated that the type of carbon source applied during incubation plays a crucial role in determining the mineral composition of the post-culture sediments. Thus, regardless of the amount of copper ion introduced to a culture with lactate as the sole carbon source, no copper sulphide was observed in the post-culture sediments. Cultures with ethanol as the sole carbon source, on the other hand, yielded covelline or digenite in all post-culture sediments.

Methanol Metabolism in Pseudomonad C

PubMed Central

Stieglitz, B.; Mateles, R. I.

1973-01-01

Cell suspensions of pseudomonad C, a bacterium capable of growth on methanol as sole carbon source, were able to oxidize methanol, formaldehyde, and formate, although the rates of oxidation for the latter two compounds were much slower. The latter compounds also could not serve as sole carbon sources. Through the use of labeled compounds, it was shown that in the presence of methanol, formaldehyde, formate, and bicarbonate were incorporated into trichloroacetic acid-precipitable material. Hexose phosphate synthetase activity was found, indicating the assimilation of methanol via an allulose pathway. No hydroxypyruvate reductase activity was found, nor was any complex membrane structure observed. Such a combination of characteristics has been observed in an obligate methylotroph (Pseudomonas W1), but pseudomonad C can utilize a variety of non-methyl substrates. Images PMID:4349032

15 CFR 930.42 - Public participation.

Code of Federal Regulations, 2011 CFR

2011-01-01

...) Specify a source for additional information, e.g., a State agency web site; and (4) Specify a contact for... sites. However, electronic notices, e.g., web sites, shall not be the sole source of a public notification, but may be used in conjunction with other means. Web sites may be used to provide a location for...

Cyclic variations in nitrogen uptake rate of soybean plants: ammonium as a nitrogen source

NASA Technical Reports Server (NTRS)

Henry, L. T.; Raper, C. D. Jr

1989-01-01

When NO3- is the sole nitrogen source in flowing solution culture, the net rate of nitrogen uptake by nonnodulated soybean (Glycine max L. Merr. cv Ransom) plants cycles between maxima and minima with a periodicity of oscillation that corresponds with the interval of leaf emergence. Since soybean plants accumulate similar quantities of nitrogen when either NH4+ or NO3- is the sole source in solution culture controlled at pH 6.0, an experiment was conducted to determine if the oscillations in net rate of nitrogen uptake also occur when NH4+ is the nitrogen source. During a 21-day period of vegetative development, net uptake of NH4+ was measured daily by ion chromatography as depletion of NH4+ from a replenished nutrient solution containing 1.0 millimolar NH4+. The net rate of NH4+ uptake oscillated with a periodicity that was similar to the interval of leaf emergence. Instances of negative net rates of uptake indicate that the transition between maxima and minima involved changes in influx and efflux components of net NH4+ uptake.

Utilization of 2,6-diaminopurine by Salmonella typhimurium.

PubMed Central

Garber, B B; Gots, J S

1980-01-01

The pathway for the utilization of 2,6-diaminopurine (DAP) as an exogenous purine source in Salmonella typhimurium was examined. In strains able to use DAP as a purine source, mutant derivatives lacking either purine nucleoside phosphorylase or adenosine deaminase activity lost the ability to do so. The implied pathway of DAP utilization was via its conversion to DAP ribonucleoside by purine nucleoside phosphorylase, followed by deamination to guanosine by adenosine deaminase. Guanosine can then enter the established purine salvage pathways. In the course of defining this pathway, purine auxotrophs able to utilize DAP as sole purine source were isolated and partially characterized. These mutants fell into several classes, including (i) strains that only required an exogenous source of guanine nucleotides (e.g., guaA and guaB strains); (ii) strains that had a purF genetic lesion (i.e., were defective in alpha-5-phosphoribosyl 1-pyrophosphate amidotransferase activity); and (iii) strains that had constitutive levels of purine nucleoside phosphorylase. Selection among purine auxotrophs blocked in the de novo synthesis of inosine 5'-monophosphate, for efficient growth on DAP as sole source of purine nucleotides, readily yielded mutants which were defective in the regulation of their deoxyribonucleoside-catabolizing enzymes (e.g., deoR mutants). PMID:6782081

48 CFR 5.202 - Exceptions.

Code of Federal Regulations, 2012 CFR

2012-10-01

... contract action results from acceptance of a proposal under the Small Business Innovation Development Act... proposal and acceptance is based solely upon the unique capability of the source to perform the particular...

48 CFR 5.202 - Exceptions.

Code of Federal Regulations, 2013 CFR

2013-10-01

... contract action results from acceptance of a proposal under the Small Business Innovation Development Act... proposal and acceptance is based solely upon the unique capability of the source to perform the particular...

Vegetation component of geothermal EIS studies: Introduced plants, ecosystem stability, and geothermal development

NASA Astrophysics Data System (ADS)

1994-10-01

This paper contributes new information about the impacts from introduced plant invasions on the native Hawaiian vegetation as consequences of land disturbance and geothermal development activities. In this regard, most geothermal development is expected to act as another recurring source of physical disturbance which favors the spread and maintenance of introduced organisms throughout the region. Where geothermal exploration and development activities extend beyond existing agricultural and residential development, they will become the initial or sole source of disturbance to the naturalized vegetation of the area. Kilauea has a unique ecosystem adapted to the dynamics of a volcanically active landscape. The characteristics of this ecosystem need to be realized in order to understand the major threats to the ecosystem and to evaluate the effects of and mitigation for geothermal development in Puna. The native Puna vegetation is well adapted to disturbances associated with volcanic eruption, but it is ill-adapted to compete with alien plant species in secondary disturbances produced by human activities. Introduced plant and animal species have become a major threat to the continued presence of the native biota in the Puna region.

Somatic embryogenesis of carrot in hormone-free medium: external pH control over morphogenesis

NASA Technical Reports Server (NTRS)

Smith, D. L.; Krikorian, A. D.

1990-01-01

Cultures of preglobular stage proembryos (PGSPs) were initiated from mechanically wounded mature zygotic embryos of carrot, Daucus carota, on a hormone-free, semisolid medium. These PGSPs have been maintained and multiplied for extended periods without their progression into later embryo stages on the same hormone-free medium containing 1 mM NH4+ as the sole nitrogen source. Sustained maintenance of cultures comprised exclusively of PGSPs was dependent on medium pH throughout the culture period. Best growth and multiplication of PGSP cultures occurred when the pH of unbuffered, hormone-free medium fell from 4.5 to 4 over a 2-week period or when buffered medium was titrated to pH 4. If the hormone-free medium was buffered to sustain a pH at or above 4.5, PGSPs developed into later embryo stages. Maintenance with continuous multiplication of PGSPs occurred equally well on medium containing NH4+ or NH4+ and NO3-, but growth was poor with NO3- alone. Additional observations on the effects of medium components such as various nitrogen sources and levels, sucrose concentration, semisolid supports, type of buffer, borate concentration, activated charcoal, and initial pH that permit optimum maintenance of the PGSPs or foster their continued developmental progression into mature embryos and plantlets are reported. The influence of the pH of the hormone-free medium as a determinant in maintaining cultures as PGSPs or allowing their continued embryonic development are unequivocally demonstrated by gross morphology, scanning electron microscopy, and histological preparations.

Microbial Thiocyanate Utilization under Highly Alkaline Conditions

PubMed Central

Sorokin, Dimitry Y.; Tourova, Tatyana P.; Lysenko, Anatoly M.; Kuenen, J. Gijs

2001-01-01

Three kinds of alkaliphilic bacteria able to utilize thiocyanate (CNS−) at pH 10 were found in highly alkaline soda lake sediments and soda soils. The first group included obligate heterotrophs that utilized thiocyanate as a nitrogen source while growing at pH 10 with acetate as carbon and energy sources. Most of the heterotrophic strains were able to oxidize sulfide and thiosulfate to tetrathionate. The second group included obligately autotrophic sulfur-oxidizing alkaliphiles which utilized thiocyanate nitrogen during growth with thiosulfate as the energy source. Genetic analysis demonstrated that both the heterotrophic and autotrophic alkaliphiles that utilized thiocyanate as a nitrogen source were related to the previously described sulfur-oxidizing alkaliphiles belonging to the gamma subdivision of the division Proteobacteria (the Halomonas group for the heterotrophs and the genus Thioalkalivibrio for autotrophs). The third group included obligately autotrophic sulfur-oxidizing alkaliphilic bacteria able to utilize thiocyanate as a sole source of energy. These bacteria could be enriched on mineral medium with thiocyanate at pH 10. Growth with thiocyanate was usually much slower than growth with thiosulfate, although the biomass yield on thiocyanate was higher. Of the four strains isolated, the three vibrio-shaped strains were genetically closely related to the previously described sulfur-oxidizing alkaliphiles belonging to the genus Thioalkalivibrio. The rod-shaped isolate differed from the other isolates by its ability to accumulate large amounts of elemental sulfur inside its cells and by its ability to oxidize carbon disulfide. Despite its low DNA homology with and substantial phenotypic differences from the vibrio-shaped strains, this isolate also belonged to the genus Thioalkalivibrio according to a phylogenetic analysis. The heterotrophic and autotrophic alkaliphiles that grew with thiocyanate as an N source possessed a relatively high level of cyanase activity which converted cyanate (CNO−) to ammonia and CO2. On the other hand, cyanase activity either was absent or was present at very low levels in the autotrophic strains grown on thiocyanate as the sole energy and N source. As a result, large amounts of cyanate were found to accumulate in the media during utilization of thiocyanate at pH 10 in batch and thiocyanate-limited continuous cultures. This is a first direct proof of a “cyanate pathway” in pure cultures of thiocyanate-degrading bacteria. Since it is relatively stable under alkaline conditions, cyanate is likely to play a role as an N buffer that keeps the alkaliphilic bacteria safe from inhibition by free ammonia, which otherwise would reach toxic levels during dissimilatory degradation of thiocyanate. PMID:11157213

Ammonium as sole N source improves grain quality in wheat.

PubMed

Fuertes-Mendizábal, Teresa; González-Torralba, Jon; Arregui, Luis M; González-Murua, Carmen; González-Moro, M Begoña; Estavillo, José M

2013-07-01

The skilful handling of N fertilizer, including N source type and its timing, is necessary to obtain maximum profitability in wheat crops in terms of production and quality. Studies on grain yield and quality with ammonium as sole N source have not yet been conducted. The aim of this study was to evaluate the effect of N source management (nitrate vs. ammonium), and splitting it into two or three amendments during the wheat life cycle, on grain yield and quality under irrigated conditions. This experiment demonstrates that Cezanne wheat plants growing with ammonium as exclusive N source are able to achieve the same yield as plants growing with nitrate and that individual wheat plants grown in irrigated pots can efficiently use late N applied in GS37. Ammonium nutrition increased both types of grain reserve proteins (gliadins and glutenins) and also increased the ratio gli/glu with respect to nitrate nutrition. The splitting of the N rate enhanced the ammonium effect on grain protein composition. The application of ammonium N source, especially when split into three amendments, has an analogous effect on grain protein content and composition to applications at a higher N rate, leading to higher N use efficiency. © 2012 Society of Chemical Industry.

Molecular characterization and analysis of the acrB gene of Aspergillus nidulans: a gene identified by genetic interaction as a component of the regulatory network that includes the CreB deubiquitination enzyme.

PubMed Central

Boase, Natasha A; Lockington, Robin A; Adams, Julian R J; Rodbourn, Louise; Kelly, Joan M

2003-01-01

Mutations in the acrB gene, which were originally selected through their resistance to acriflavine, also result in reduced growth on a range of sole carbon sources, including fructose, cellobiose, raffinose, and starch, and reduced utilization of omega-amino acids, including GABA and beta-alanine, as sole carbon and nitrogen sources. The acrB2 mutation suppresses the phenotypic effects of mutations in the creB gene that encodes a regulatory deubiquitinating enzyme, and in the creC gene that encodes a WD40-repeat-containing protein. Thus AcrB interacts with a regulatory network controlling carbon source utilization that involves ubiquitination and deubiquitination. The acrB gene was cloned and physically analyzed, and it encodes a novel protein that contains three putative transmembrane domains and a coiled-coil region. AcrB may play a role in the ubiquitination aspect of this regulatory network. PMID:12750323

Constitutive cellulase production from glucose using the recombinant Trichoderma reesei strain overexpressing an artificial transcription activator.

PubMed

Zhang, Xiaoyue; Li, Yonghao; Zhao, Xinqing; Bai, Fengwu

2017-01-01

The high cost of cellulase production presents biggest challenge in biomass deconstruction. Cellulase production by Trichoderma reesei using low cost carbon source is of great interest. In this study, an artificial transcription activator containing the Cre1 binding domain linked to the Xyr1 effector and binding domains was designed and constitutively overexpressed in T. reesei RUT C30. The recombinant strain T. reesei zxy-2 displayed constitutive cellulase production using glucose as a sole carbon source, and the production titer was 12.75-fold of that observed with T. reesei RUT C30 in shake flask culture. Moreover, FPase and xylanase titers of 2.63 and 108.72IU/mL, respectively, were achieved using glucose as sole carbon source within 48h in a 7-L fermenter by batch fermentation using T. reesei zxy-2. The crude enzyme obtained was used to hydrolyze alkali pretreated corn stover, and a high glucose yield of 99.18% was achieved. Copyright © 2016. Published by Elsevier Ltd.

Biodegradation of Polyester Polyurethane by Endophytic Fungi▿

PubMed Central

Russell, Jonathan R.; Huang, Jeffrey; Anand, Pria; Kucera, Kaury; Sandoval, Amanda G.; Dantzler, Kathleen W.; Hickman, DaShawn; Jee, Justin; Kimovec, Farrah M.; Koppstein, David; Marks, Daniel H.; Mittermiller, Paul A.; Núñez, Salvador Joel; Santiago, Marina; Townes, Maria A.; Vishnevetsky, Michael; Williams, Neely E.; Vargas, Mario Percy Núñez; Boulanger, Lori-Ann; Bascom-Slack, Carol; Strobel, Scott A.

2011-01-01

Bioremediation is an important approach to waste reduction that relies on biological processes to break down a variety of pollutants. This is made possible by the vast metabolic diversity of the microbial world. To explore this diversity for the breakdown of plastic, we screened several dozen endophytic fungi for their ability to degrade the synthetic polymer polyester polyurethane (PUR). Several organisms demonstrated the ability to efficiently degrade PUR in both solid and liquid suspensions. Particularly robust activity was observed among several isolates in the genus Pestalotiopsis, although it was not a universal feature of this genus. Two Pestalotiopsis microspora isolates were uniquely able to grow on PUR as the sole carbon source under both aerobic and anaerobic conditions. Molecular characterization of this activity suggests that a serine hydrolase is responsible for degradation of PUR. The broad distribution of activity observed and the unprecedented case of anaerobic growth using PUR as the sole carbon source suggest that endophytes are a promising source of biodiversity from which to screen for metabolic properties useful for bioremediation. PMID:21764951

Aerobic degradation of 2,4,6-trinitrotoluene by Enterobacter cloacae PB2 and by pentaerythritol tetranitrate reductase

DOE Office of Scientific and Technical Information (OSTI.GOV)

French, C.E.; Bruce, N.C.; Nicklin, S.

1998-08-01

Enterobacter cloacae PB2 was originally isolated on the basis of its ability to utilize nitrate esters, such as pentaerythritol tetranitrate (PETN) and glycerol trinitrate, as the sole nitrogen source for growth. The enzyme responsible is an NADPH-dependent reductase designated PETN reductase. E. cloacae PB2 was found to be capable of slow aerobic growth with 2,4,6-trinitrotoluene (TNT) as the sole nitrogen source. Dinitrotoluenes were not produced and could not be used as nitrogen sources. Purified PETN reductase was found to reduce TNT to its hydride-Meisenheimer complex, which was further reduced to the dihydride-Meisenheimer complex. Purified PETN reductase and recombinant Escherichia colimore » expressing PETN reductase were able to liberate nitrogen as nitrite from TNT. The ability to remove nitrogen from TNT suggests that PB2 or recombinant organisms expressing PETN reductase may be useful for bioremediation of TNT-contaminated soil and water.« less

Biodegradation of polyester polyurethane by endophytic fungi.

PubMed

Russell, Jonathan R; Huang, Jeffrey; Anand, Pria; Kucera, Kaury; Sandoval, Amanda G; Dantzler, Kathleen W; Hickman, DaShawn; Jee, Justin; Kimovec, Farrah M; Koppstein, David; Marks, Daniel H; Mittermiller, Paul A; Núñez, Salvador Joel; Santiago, Marina; Townes, Maria A; Vishnevetsky, Michael; Williams, Neely E; Vargas, Mario Percy Núñez; Boulanger, Lori-Ann; Bascom-Slack, Carol; Strobel, Scott A

2011-09-01

Bioremediation is an important approach to waste reduction that relies on biological processes to break down a variety of pollutants. This is made possible by the vast metabolic diversity of the microbial world. To explore this diversity for the breakdown of plastic, we screened several dozen endophytic fungi for their ability to degrade the synthetic polymer polyester polyurethane (PUR). Several organisms demonstrated the ability to efficiently degrade PUR in both solid and liquid suspensions. Particularly robust activity was observed among several isolates in the genus Pestalotiopsis, although it was not a universal feature of this genus. Two Pestalotiopsis microspora isolates were uniquely able to grow on PUR as the sole carbon source under both aerobic and anaerobic conditions. Molecular characterization of this activity suggests that a serine hydrolase is responsible for degradation of PUR. The broad distribution of activity observed and the unprecedented case of anaerobic growth using PUR as the sole carbon source suggest that endophytes are a promising source of biodiversity from which to screen for metabolic properties useful for bioremediation.

21 CFR 520.2158a - Streptomycin sulfate oral solution.

Code of Federal Regulations, 2011 CFR

2011-04-01

... sole source of streptomycin. Warning: Certain strains of bacteria may develop a tolerance for...: Certain strains of bacteria may develop a tolerance for streptomycin. Consult a veterinarian or animal...

21 CFR 520.2158a - Streptomycin sulfate oral solution.

Code of Federal Regulations, 2010 CFR

2010-04-01

... sole source of streptomycin. Warning: Certain strains of bacteria may develop a tolerance for...: Certain strains of bacteria may develop a tolerance for streptomycin. Consult a veterinarian or animal...

21 CFR 522.2240 - Sulfaethoxypyridazine.

Code of Federal Regulations, 2011 CFR

2011-04-01

... of respiratory infection (pneumonia, shipping fever), foot rot, calf scours; as adjunctive therapy in... sulfaethoxypyridazine; do not treat within 16 days of slaughter; as sole source of sulfonamide; milk that has been taken...

48 CFR 5.202 - Exceptions.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Innovation Development Act of 1982 (Pub. L. 97-219); (8) The proposed contract action results from the... unsolicited research proposal and acceptance is based solely upon the unique capability of the source to...

48 CFR 5.202 - Exceptions.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Innovation Development Act of 1982 (Pub. L. 97-219); (8) The proposed contract action results from the... unsolicited research proposal and acceptance is based solely upon the unique capability of the source to...

Repeated-batch fermentations of xylose and glucose-xylose mixtures using a respiration-deficient Saccharomyces cerevisiae engineered for xylose metabolism.

PubMed

Kim, Soo Rin; Lee, Ki-Sung; Choi, Jin-Ho; Ha, Suk-Jin; Kweon, Dae-Hyuk; Seo, Jin-Ho; Jin, Yong-Su

2010-11-01

Xylose-fermenting Saccharomyces strains are needed for commercialization of ethanol production from lignocellulosic biomass. Engineered Saccharomyces cerevisiae strains expressing XYL1, XYL2 and XYL3 from Pichia stipitis, however, utilize xylose in an oxidative manner, which results in significantly lower ethanol yields from xylose as compared to glucose. As such, we hypothesized that reconfiguration of xylose metabolism from oxidative into fermentative manner might lead to efficient ethanol production from xylose. To this end, we generated a respiration-deficient (RD) mutant in order to enforce engineered S. cerevisiae to utilize xylose only through fermentative metabolic routes. Three different repeated-batch fermentations were performed to characterize characteristics of the respiration-deficient mutant. When fermenting glucose as a sole carbon source, the RD mutant exhibited near theoretical ethanol yields (0.46 g g(-1)) during repeated-batch fermentations by recycling the cells. As the repeated-batch fermentation progressed, the volumetric ethanol productivity increased (from 7.5 to 8.3 g L(-1)h(-1)) because of the increased biomass from previous cultures. On the contrary, the mutant showed decreasing volumetric ethanol productivities during the repeated-batch fermentations using xylose as sole carbon source (from 0.4 to 0.3 g L(-1)h(-1)). The mutant did not grow on xylose and lost fermenting ability gradually, indicating that the RD mutant cannot maintain a good fermenting ability on xylose as a sole carbon source. However, the RD mutant was capable of fermenting a mixture of glucose and xylose with stable yields (0.35 g g(-1)) and productivities (0.52 g L(-1)h(-1)) during the repeated-batch fermentation. In addition, ethanol yields from xylose during the mixed sugar fermentation (0.30 g g(-1)) were higher than ethanol yields from xylose as a sole carbon source (0.21 g g(-1)). These results suggest that a strategy for increasing ethanol yield through respiration-deficiency can be applied for the fermentation of lignocellulosic hydrolyzates containing glucose and xylose. Copyright © 2010 Elsevier B.V. All rights reserved.

26 CFR 1.501(c)(7)-1 - Social clubs.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 26 Internal Revenue 7 2013-04-01 2013-04-01 false Social clubs. 1.501(c)(7)-1 Section 1.501(c)(7... (CONTINUED) INCOME TAXES (CONTINUED) Exempt Organizations § 1.501(c)(7)-1 Social clubs. (a) The exemption... general, this exemption extends to social and recreation clubs which are supported solely by membership...

26 CFR 1.501(c)(7)-1 - Social clubs.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 26 Internal Revenue 7 2012-04-01 2012-04-01 false Social clubs. 1.501(c)(7)-1 Section 1.501(c)(7... (CONTINUED) INCOME TAXES (CONTINUED) Exempt Organizations § 1.501(c)(7)-1 Social clubs. (a) The exemption... general, this exemption extends to social and recreation clubs which are supported solely by membership...

Biodegradable Core-shell Dual-Metal-Organic-Frameworks Nanotheranostic Agent for Multiple Imaging Guided Combination Cancer Therapy

PubMed Central

Wang, Dongdong; Zhou, Jiajia; Shi, Ruohong; Wu, Huihui; Chen, Ruhui; Duan, Beichen; Xia, Guoliang; Xu, Pengping; Wang, Hui; Zhou, Shu; Wang, Chengming; Wang, Haibao; Guo, Zhen; Chen, Qianwang

2017-01-01

Metal-organic-frameworks (MOFs) possess high porosity, large surface area, and tunable functionality are promising candidates for synchronous diagnosis and therapy in cancer treatment. Although large number of MOFs has been discovered, conventional MOF-based nanoplatforms are mainly limited to the sole MOF source with sole functionality. In this study, surfactant modified Prussian blue (PB) core coated by compact ZIF-8 shell (core-shell dual-MOFs, CSD-MOFs) has been reported through a versatile stepwise approach. With Prussian blue as core, CSD-MOFs are able to serve as both magnetic resonance imaging (MRI) and fluorescence optical imaging (FOI) agents. We show that CSD-MOFs crystals loading the anticancer drug doxorubicin (DOX) are efficient pH and near-infrared (NIR) dual-stimuli responsive drug delivery vehicles. After the degradation of ZIF-8, simultaneous NIR irradiation to the inner PB MOFs continuously generate heat that kill cancer cells. Their efficacy on HeLa cancer cell lines is higher compared with the respective single treatment modality, achieving synergistic chemo-thermal therapy efficacy. In vivo results indicate that the anti-tumor efficacy of CSD-MOFs@DOX+NIR was 7.16 and 5.07 times enhanced compared to single chemo-therapy and single thermal-therapy respectively. Our strategy opens new possibilities to construct multifunctional theranostic systems through integration of two different MOFs. PMID:29158848

Mollusc-Algal Chloroplast Endosymbiosis. Photosynthesis, Thylakoid Protein Maintenance, and Chloroplast Gene Expression Continue for Many Months in the Absence of the Algal Nucleus1

PubMed Central

Green, Brian J.; Li, Wei-Ye; Manhart, James R.; Fox, Theodore C.; Summer, Elizabeth J.; Kennedy, Robert A.; Pierce, Sidney K.; Rumpho, Mary E.

2000-01-01

Early in its life cycle, the marine mollusc Elysia chlorotica Gould forms an intracellular endosymbiotic association with chloroplasts of the chromophytic alga Vaucheria litorea C. Agardh. As a result, the dark green sea slug can be sustained in culture solely by photoautotrophic CO2 fixation for at least 9 months if provided with only light and a source of CO2. Here we demonstrate that the sea slug symbiont chloroplasts maintain photosynthetic oxygen evolution and electron transport activity through photosystems I and II for several months in the absence of any external algal food supply. This activity is correlated to the maintenance of functional levels of chloroplast-encoded photosystem proteins, due in part at least to de novo protein synthesis of chloroplast proteins in the sea slug. Levels of at least one putative algal nuclear encoded protein, a light-harvesting complex protein homolog, were also maintained throughout the 9-month culture period. The chloroplast genome of V. litorea was found to be 119.1 kb, similar to that of other chromophytic algae. Southern analysis and polymerase chain reaction did not detect an algal nuclear genome in the slug, in agreement with earlier microscopic observations. Therefore, the maintenance of photosynthetic activity in the captured chloroplasts is regulated solely by the algal chloroplast and animal nuclear genomes. PMID:10982447

Mollusc-algal chloroplast endosymbiosis. Photosynthesis, thylakoid protein maintenance, and chloroplast gene expression continue for many months in the absence of the algal nucleus.

PubMed

Green, B J; Li, W Y; Manhart, J R; Fox, T C; Summer, E J; Kennedy, R A; Pierce, S K; Rumpho, M E

2000-09-01

Early in its life cycle, the marine mollusc Elysia chlorotica Gould forms an intracellular endosymbiotic association with chloroplasts of the chromophytic alga Vaucheria litorea C. Agardh. As a result, the dark green sea slug can be sustained in culture solely by photoautotrophic CO(2) fixation for at least 9 months if provided with only light and a source of CO(2). Here we demonstrate that the sea slug symbiont chloroplasts maintain photosynthetic oxygen evolution and electron transport activity through photosystems I and II for several months in the absence of any external algal food supply. This activity is correlated to the maintenance of functional levels of chloroplast-encoded photosystem proteins, due in part at least to de novo protein synthesis of chloroplast proteins in the sea slug. Levels of at least one putative algal nuclear encoded protein, a light-harvesting complex protein homolog, were also maintained throughout the 9-month culture period. The chloroplast genome of V. litorea was found to be 119.1 kb, similar to that of other chromophytic algae. Southern analysis and polymerase chain reaction did not detect an algal nuclear genome in the slug, in agreement with earlier microscopic observations. Therefore, the maintenance of photosynthetic activity in the captured chloroplasts is regulated solely by the algal chloroplast and animal nuclear genomes.

31 CFR 539.504 - Departments and agencies of the United States Government.

Code of Federal Regulations, 2012 CFR

2012-07-01

... requirements under defense production agreements; intelligence requirements; sole source suppliers, spare parts... Office of Chemical, Biological and Missile Nonproliferation and the Office of the Assistant Legal Adviser...

31 CFR 539.504 - Departments and agencies of the United States Government.

Code of Federal Regulations, 2011 CFR

2011-07-01

... requirements under defense production agreements; intelligence requirements; sole source suppliers, spare parts... Office of Chemical, Biological and Missile Nonproliferation and the Office of the Assistant Legal Adviser...

31 CFR 539.504 - Departments and agencies of the United States Government.

Code of Federal Regulations, 2014 CFR

2014-07-01

... requirements under defense production agreements; intelligence requirements; sole source suppliers, spare parts... Office of Chemical, Biological and Missile Nonproliferation and the Office of the Assistant Legal Adviser...

31 CFR 539.504 - Departments and agencies of the United States Government.

Code of Federal Regulations, 2013 CFR

2013-07-01

... requirements under defense production agreements; intelligence requirements; sole source suppliers, spare parts... Office of Chemical, Biological and Missile Nonproliferation and the Office of the Assistant Legal Adviser...

Wildlife

Treesearch

Norbert V. DeByle

1985-01-01

Aspen forests provide important habitat for many species of wildlife (Gullion 1977b), especially in the West (see the appendix to this chapter). In the coniferous forests of the interior West, aspen groves may be the only source of abundant forage; in the grasslands they may be the sole source of cover. A primary value of the aspen ecosystem in the West during the past...

Effects of feeding hulled and hull-less barley with low- and high-forage diets on lactation performance, nutrient digestibility, and milk fatty acid composition of lactating dairy cows.

PubMed

Yang, Y; Ferreira, G; Teets, C L; Corl, B A; Thomason, W E; Griffey, C A

2018-04-01

The objective of this study was to evaluate lactation performance, nutrient digestibility, and milk fatty acid composition of high-producing dairy cows consuming diets containing hulled or hull-less barley as the grain source when feeding low-forage (LF) or high-forage (HF) diets. Eight primiparous (610 ± 40 kg of body weight and 72 ± 14 d in milk) and 16 multiparous (650 ± 58 kg of body weight and 58 ± 16 d in milk) Holstein cows were randomly assigned to 1 of 4 diets in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments and 21-d periods. Cows were assigned to squares based on parity (1, 2, and ≥3) and days in milk. Diets were formulated to contain on a dry matter basis (1) 45% forage and hulled barley as the sole grain source, (2) 65% forage and hulled barley as the sole grain source, (3) 45% forage and hull-less barley as the sole grain source, and (4) 65% forage and hull-less barley as the sole grain source. Dry matter intake tended to be lower for the diet with 65% forage and hulled barley than for the rest of the diets (24.4 vs. 26.6 kg/d). Neither the type of barley nor the forage-to-concentrate ratio affected milk yield (41.7 kg/d). Barley type did not affect milk fat or protein concentrations. Feeding LF diets decreased milk fat concentration from 3.91% to 3.50%. This decrease was less than anticipated and resulted in a 7% decrease in milk fat yield relative to cows consuming HF diets (1.60 and 1.49 kg/d for HF and LF diets, respectively). Feeding LF diets increased the concentration of C18:1 trans-10 in milk fat, suggesting that feeding LF diets may have marginally altered rumen function. In conclusion, LF diets containing barley grains can marginally decrease milk fat concentration. Overall, and based on the conditions of this study, there is limited evidence to anticipate a dramatic or acute milk fat depression when feeding hull-less barley as the grain source in diets for high-producing dairy cows. Copyright © 2018 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

48 CFR 873.104 - Competition requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... be approved if determined legally to be associated with affiliated institutions), or from blood banks, organ banks, or research centers, the resource may be acquired on a sole source basis. (38 U.S.C. 8153...

13 CFR 126.600 - What are HUBZone contracts?

Code of Federal Regulations, 2010 CFR

2010-01-01

... qualified HUBZone SBC through any of the following procurement methods: (a) Sole source awards to qualified...) Awards to qualified HUBZone SBCs through full and open competition after a price evaluation preference in...

46 CFR 112.35-7 - Activating means.

Code of Federal Regulations, 2010 CFR

2010-10-01

... POWER SYSTEMS Manually Controlled Emergency Systems Having a Storage Battery or a Diesel Engine or Gas Turbine Driven Generator as the Sole Emergency Power Source § 112.35-7 Activating means. The activating...

46 CFR 112.35-7 - Activating means.

Code of Federal Regulations, 2011 CFR

2011-10-01

... POWER SYSTEMS Manually Controlled Emergency Systems Having a Storage Battery or a Diesel Engine or Gas Turbine Driven Generator as the Sole Emergency Power Source § 112.35-7 Activating means. The activating...

Emerging contaminants at a closed and an operating landfill in Oklahoma

USGS Publications Warehouse

Andrews, William J.; Masoner, Jason R.; Cozzarelli, Isabelle M.

2012-01-01

Landfills are the final depositories for a wide range of solid waste from both residential and commercial sources, and therefore have the potential to produce leachate containing many organic compounds found in consumer products such as pharmaceuticals, plasticizers, disinfectants, cleaning agents, fire retardants, flavorings, and preservatives, known as emerging contaminants (ECs). Landfill leachate was sampled from landfill cells of three different age ranges from two landfills in Central Oklahoma. Samples were collected from an old cell containing solid waste greater than 25 years old, an intermediate age cell with solid waste between 16 and 3 years old, and operating cell with solid waste less than 5 years old to investigate the chemical variability and persistence of selected ECs in landfill leachate of differing age sources. Twenty-eight of 69 analyzed ECs were detected in one or more samples from the three leachate sources. Detected ECs ranged in concentration from 0.11 to 114 μg/L and included 4 fecal and plant sterols, 13 household\\industrial, 7 hydrocarbon, and 4 pesticide compounds. Four ECs were solely detected in the oldest leachate sample, two ECs were solely detected in the intermediate leachate sample, and no ECs were solely detected in the youngest leachate sample. Eleven ECs were commonly detected in all three leachate samples and are an indication of the contents of solid waste deposited over several decades and the relative resistance of some ECs to natural attenuation processes in and near landfills.

Stable isotopes in juvenile marine fishes and their invertebrate prey from the Thames Estuary, UK, and adjacent coastal regions

NASA Astrophysics Data System (ADS)

Leakey, Chris D. B.; Attrill, Martin J.; Jennings, Simon; Fitzsimons, Mark F.

2008-04-01

Estuaries are regarded as valuable nursery habitats for many commercially important marine fishes, potentially providing a thermal resource, refuge from predators and a source of abundant prey. Stable isotope analysis may be used to assess relative resource use from isotopically distinct sources. This study comprised two major components: (1) development of a spatial map and discriminant function model of stable isotope variation in selected invertebrate groups inhabiting the Thames Estuary and adjacent coastal regions; and (2) analysis of stable isotope signatures of juvenile bass ( Dicentrarchus labrax), sole ( Solea solea) and whiting ( Merlangius merlangus) for assessment of resource use and feeding strategies. The data were also used to consider anthropogenic enrichment of the estuary and potential energetic benefits of feeding in estuarine nursery habitat. Analysis of carbon (δ 13C), nitrogen (δ 15N) and sulphur (δ 34S) isotope data identified significant differences in the 'baseline' isotopic signatures between estuarine and coastal invertebrates, and discriminant function analysis allowed samples to be re-classified to estuarine and coastal regions with 98.8% accuracy. Using invertebrate signatures as source indicators, stable isotope data classified juvenile fishes to the region in which they fed. Feeding signals appear to reflect physiological (freshwater tolerance) and functional (mobility) differences between species. Juvenile sole were found to exist as two isotopically-discrete sub-populations, with no evidence of mixing between the two. An apparent energetic benefit of estuarine feeding was only found for sole.

Isolation and characterization of heterotrophic bacteria able to grow aerobically with quaternary ammonium alcohols as sole source of carbon and nitrogen.

PubMed

Kaech, Andres; Vallotton, Nathalie; Egli, Thomas

2005-04-01

The quaternary ammonium alcohols (QAAs) 2,3-dihydroxypropyl-trimethyl-ammonium (TM), dimethyl-diethanol-ammonium (DM) and methyl-triethanol-ammonium (MM) are hydrolysis products of their parent esterquat surfactants, which are widely used as softeners in fabric care. We isolated several bacteria growing with QAAs as the sole source of carbon and nitrogen. The strains were compared with a previously isolated TM-degrading bacterium, which was identified as a representative of the species Pseudomonas putida (Syst. Appl. Microbiol. 24 (2001) 252). Two bacteria were isolated with DM, referred to as strains DM 1 and DM 2, respectively. Based on 16S-rDNA analysis, they provided 97% (DM 1) and 98% (DM 2) identities to the closest related strain Zoogloea ramigera Itzigsohn 1868AL. Both strains were long, slim, motile rods but only DM 1 showed the floc forming activity, which is typical for representatives of the genus Zoogloea. Using MM we isolated a Gram-negative, non-motile rod referred to as strain MM 1. The 16S-rDNA sequence of the isolated bacterium revealed 94% identities (best match) to Rhodobacter sphaeroides only. The strains MM 1 and DM 1 exclusively grew with the QAA which was used for their isolation. DM 2 was also utilizing TM as sole source of carbon and nitrogen. However, all of the isolated bacteria were growing with the natural and structurally related compound choline.

Anaerobic oxidation of 2-chloroethanol under denitrifying conditions by Pseudomonas stutzeri strain JJ.

PubMed

Dijk, J A; Stams, A J M; Schraa, G; Ballerstedt, H; de Bont, J A M; Gerritse, J

2003-11-01

A bacterium that uses 2-chloroethanol as sole energy and carbon source coupled to denitrification was isolated from 1,2-dichloroethane-contaminated soil. Its 16 S rDNA sequence showed 98% similarity with the type strain of Pseudomonas stutzeri (DSM 5190) and the isolate was tentatively identified as Pseudomonas stutzeri strain JJ. Strain JJ oxidized 2-chloroethanol completely to CO(2) with NO(3)(- )or O(2) as electron acceptor, with a preference for O(2) if supplied in combination. Optimum growth on 2-chloroethanol with nitrate occurred at 30 degrees C with a mu(max) of 0.14 h(-1) and a yield of 4.4 g protein per mol 2-chloroethanol metabolized. Under aerobic conditions, the mu(max) was 0.31 h(-1). NO(2)(-) also served as electron acceptor, but reduction of Fe(OH)(3), MnO(2), SO(4)(2-), fumarate or ClO(3)(-) was not observed. Another chlorinated compound used as sole energy and carbon source under aerobic and denitrifying conditions was chloroacetate. Various different bacterial strains, including some closely related Pseudomonas stutzeri strains, were tested for their ability to grow on 2-chloroethanol as sole energy and carbon source under aerobic and denitrifying conditions, respectively. Only three strains, Pseudomonas stutzeri strain LMD 76.42, Pseudomonas putida US2 and Xanthobacter autotrophicus GJ10, grew aerobically on 2-chloroethanol. This is the first report of oxidation of 2-chloroethanol under denitrifying conditions by a pure bacterial culture.

Scale-up of an alkaline protease from Bacillus pumilus MTCC 7514 utilizing fish meal as a sole source of nutrients.

PubMed

Gupta, Rishikesh Kumar; Prasad, Dinesh; Sathesh, Jaykumar; Naidu, Ramachandra Boopathy; Kamini, Numbi Ramudu; Palanivel, Saravanan; Gowthaman, Marichetti Kuppuswami

2012-09-01

Fish meal grades SL1 and SL2 from Sardine (Sardinella longiceps) and NJ from Pink Perch (Nemipterus japonicas) were evaluated as a sole source of carbon and nitrogen in the medium for alkaline protease production by Bacillus pumilus MTCC 7514. The analysis of the fish meal suggests that the carbon and nitrogen contents in fish meal are sufficient to justify its choice as replacement for other nutrients. Protease production increased significantly (4,914 U/ml) in medium containing only fish meal, compared with the basal medium (2,646 U/ml). However, the elimination of inorganic salts from media reduced the protease productivity. In addition, all the three grades of fish meal yielded almost the same amounts of protease when employed as the sole source of carbon and nitrogen. Nevertheless, the best results were observed in fish meal SL1 medium. Furthermore, protease production was enhanced to 6,966 U/ml and 7,047 U/ml on scaling up from flask (4,914 U/ml) to 3.7 and 20 L fermenters, respectively, using fish meal (10 g/l). Similarly, the corresponding improvement in productivities over flask (102.38 U/ml/h) was 193.5 and 195.75 U/ml/h in 3.7 and 20 L fermenters, respectively. The crude protease was found to have dehairing ability in leather processing, which is bound to have great environmental benefits.

Enhanced biological phosphorus removal in a sequencing batch reactor using propionate as the sole carbon source.

PubMed

Pijuan, M; Saunders, A M; Guisasola, A; Baeza, J A; Casas, C; Blackall, L L

2004-01-05

An enhanced biological phosphorus removal (EBPR) system was developed in a sequencing batch reactor (SBR) using propionate as the sole carbon source. The microbial community was followed using fluorescence in situ hybridization (FISH) techniques and Candidatus 'Accumulibacter phosphatis' were quantified from the start up of the reactor until steady state. A series of SBR cycle studies was performed when 55% of the SBR biomass was Accumulibacter, a confirmed polyphosphate accumulating organism (PAO) and when Candidatus 'Competibacter phosphatis', a confirmed glycogen-accumulating organism (GAO), was essentially undetectable. These experiments evaluated two different carbon sources (propionate and acetate), and in every case, two different P-release rates were detected. The highest rate took place while there was volatile fatty acid (VFA) in the mixed liquor, and after the VFA was depleted a second P-release rate was observed. This second rate was very similar to the one detected in experiments performed without added VFA.A kinetic and stoichiometric model developed as a modification of Activated Sludge Model 2 (ASM2) including glycogen economy, was fitted to the experimental profiles. The validation and calibration of this model was carried out with the cycle study experiments performed using both VFAs. The effect of pH from 6.5 to 8.0 on anaerobic P-release and VFA-uptake and aerobic P-uptake was also studied using propionate. The optimal overall working pH was around 7.5. This is the first study of the microbial community involved in EBPR developed with propionate as a sole carbon source along with detailed process performance investigations of the propionate-utilizing PAOs. Copyright 2003 Wiley Periodicals, Inc.

Addendum to the Final Audit Report on Procurement of Spare Parts and Supplies

DTIC Science & Technology

1993-06-04

Contraves USA, Simulation and Systems Integration, Tampa, FL Contract No.: N00104-89-G-A050-4009 Awarded: April 20, 1990 Procurement Method...awarded sole source to Contraves . The amplifier is a source-controlled item, and Contraves is the only approved source. There are no commercial...The total contract value was $69,552. Contraves assembled and tested the amplifiers. For the subject procurement, Contraves paid $* per unit for the

26 CFR 1.514(c)-1 - Acquisition indebtedness.

Code of Federal Regulations, 2013 CFR

2013-04-01

... continue current operations. Example 3. (a) U, an exempt private preparatory school, as its sole... as a down payment from Y and takes back a purchase money mortgage of $2 million which bears interest...

26 CFR 1.514(c)-1 - Acquisition indebtedness.

Code of Federal Regulations, 2012 CFR

2012-04-01

... continue current operations. Example 3. (a) U, an exempt private preparatory school, as its sole... as a down payment from Y and takes back a purchase money mortgage of $2 million which bears interest...

26 CFR 1.514(c)-1 - Acquisition indebtedness.

Code of Federal Regulations, 2014 CFR

2014-04-01

... continue current operations. Example 3. (a) U, an exempt private preparatory school, as its sole... as a down payment from Y and takes back a purchase money mortgage of $2 million which bears interest...

26 CFR 1.514(c)-1 - Acquisition indebtedness.

Code of Federal Regulations, 2011 CFR

2011-04-01

... continue current operations. Example 3. (a) U, an exempt private preparatory school, as its sole... as a down payment from Y and takes back a purchase money mortgage of $2 million which bears interest...

Bioreactors for removing methyl bromide following contained fumigations

USGS Publications Warehouse

Miller, L.G.; Baesman, S.M.; Oremland, R.S.

2003-01-01

Use of methyl bromide (MeBr) as a quarantine, commodity, or structural fumigant is under scrutiny because its release to the atmosphere contributes to the depletion of stratospheric ozone. A closed-system bioreactor consisting of 0.5 L of a growing culture of a previously described bacterium, strain IMB-1, removed MeBr (> 110 ??mol L-1) from recirculating air. Strain IMB-1 grew slowly to high cell densities in the bioreactor using MeBr as its sole carbon and energy source. Bacterial oxidation of MeBr produced CO2 and hydrobromic acid (HBr), which required continuous neutralization with NaOH for the system to operate effectively. Strain IMB-1 was capable of sustained oxidation of large amounts of MeBr (170 mmol in 46 d). In an open-system bioreactor (10-L fermenter), strain IMB-1 oxidized a continuous supply of MeBr (220 ??mol L-1 in air). Growth was continuous, and 0.5 mol of MeBr was removed from the air supply in 14 d. The specific rate of MeBr oxidation was 7 ?? 10-16 mol cell-1 h-1. Bioreactors such as these can therefore be used to remove large quantities of contaminant MeBr, which opens the possibility of biodegradation as a practical means for its disposal.

76 FR 35842 - Endangered and Threatened Species; Take of Anadromous Fish

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-20

... populations. These recommendations are not formal ESA standards nor will they constitute the sole source of.... However, debating the relative magnitude of improvements in productivity that might occur from a given set...

48 CFR 1513.170-1 - Contents of sole source justifications.

Code of Federal Regulations, 2010 CFR

2010-10-01

... the micro-purchase threshold. The statement must cite one or more of the circumstances in FAR 6.302 and the necessary facts to support each circumstance. Although program offices may not cite the...

13 CFR 124.403 - How is a business plan updated and modified?

Code of Federal Regulations, 2011 CFR

2011-01-01

... aggregate dollar value of 8(a) contracts to be sought, broken down by sole source and competitive... primary industry classification falls within that Major Group. Any adjustment will take into account...

21 CFR 558.369 - Nitarsone.

Code of Federal Regulations, 2014 CFR

2014-04-01

... edible tissues. The drug is dangerous for ducks, geese, and dogs. Overdosage or lack of water may result... dangerous for ducks, geese, and dogs. Use as sole source of arsenic. [46 FR 47535, Sept. 29, 1981, as...

21 CFR 558.369 - Nitarsone.

Code of Federal Regulations, 2013 CFR

2013-04-01

... edible tissues. The drug is dangerous for ducks, geese, and dogs. Overdosage or lack of water may result... dangerous for ducks, geese, and dogs. Use as sole source of arsenic. [46 FR 47535, Sept. 29, 1981, as...

21 CFR 558.369 - Nitarsone.

Code of Federal Regulations, 2010 CFR

2010-04-01

... edible tissues. The drug is dangerous for ducks, geese, and dogs. Overdosage or lack of water may result... dangerous for ducks, geese, and dogs. Use as sole source of arsenic. [46 FR 47535, Sept. 29, 1981, as...

21 CFR 558.369 - Nitarsone.

Code of Federal Regulations, 2012 CFR

2012-04-01

... edible tissues. The drug is dangerous for ducks, geese, and dogs. Overdosage or lack of water may result... dangerous for ducks, geese, and dogs. Use as sole source of arsenic. [46 FR 47535, Sept. 29, 1981, as...

21 CFR 558.369 - Nitarsone.

Code of Federal Regulations, 2011 CFR

2011-04-01

... edible tissues. The drug is dangerous for ducks, geese, and dogs. Overdosage or lack of water may result... dangerous for ducks, geese, and dogs. Use as sole source of arsenic. [46 FR 47535, Sept. 29, 1981, as...

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shao, Jiahang; Antipov, Sergey P.; Baryshev, Sergey V.

Field emission from a solid metal surface has been continuously studied for a century over macroscopic to atomic scales. It is general knowledge that, other than the surface properties, the emitted current is governed solely by the applied electric field. A pin cathode has been used to study the dependence of field emission on stored energy in an L-band rf gun. The stored energy was changed by adjusting the axial position (distance between the cathode base and the gun back surface) of the cathode while the applied electric field on the cathode tip is kept constant. Avery strong correlation ofmore » the field-emission current with the stored energy has been observed. While eliminating all possible interfering sources, an enhancement of the current by a factor of 5 was obtained as the stored energy was increased by a factor of 3. It implies that under certain circumstances a localized field emission may be significantly altered by the global parameters in a system.« less

A Small Acoustic Goniometer for General Purpose Research

PubMed Central

Pook, Michael L.; Loo, Sin Ming

2016-01-01

Understanding acoustic events and monitoring their occurrence is a useful aspect of many research projects. In particular, acoustic goniometry allows researchers to determine the source of an event based solely on the sound it produces. The vast majority of acoustic goniometry research projects used custom hardware targeted to the specific application under test. Unfortunately, due to the wide range of sensing applications, a flexible general purpose hardware/firmware system does not exist for this purpose. This article focuses on the development of such a system which encourages the continued exploration of general purpose hardware/firmware and lowers barriers to research in projects requiring the use of acoustic goniometry. Simulations have been employed to verify system feasibility, and a complete hardware implementation of the acoustic goniometer has been designed and field tested. The results are reported, and suggested areas for improvement and further exploration are discussed. PMID:27136563

Versatile Cellulose-Based Carbon Aerogel for the Removal of Both Cationic and Anionic Metal Contaminants from Water.

PubMed

Alatalo, Sara-Maaria; Pileidis, Filoklis; Mäkilä, Ermei; Sevilla, Marta; Repo, Eveliina; Salonen, Jarno; Sillanpää, Mika; Titirici, Maria-Magdalena

2015-11-25

Hydrothermal carbonization of cellulose in the presence of the globular protein ovalbumin leads to the formation of nitrogen-doped carbon aerogel with a fibrillar continuous carbon network. The protein plays here a double role: (i) a natural source of nitrogen functionalities (2.1 wt %) and (ii) structural directing agent (S(BET) = 38 m(2)/g). The applicability in wastewater treatment, namely, for heavy metal removal, was examined through adsorption of Cr(VI) and Pb(II) ion solely and in a mixed bicomponent aqueous solutions. This cellulose-based carbogel shows an enhanced ability to remove both Cr(VI) (∼68 mg/g) and Pb(II) (∼240 mg/g) from the targeted solutions in comparison to other carbon materials reported in the literature. The presence of competing ions showed little effect on the adsorption efficiency toward Cr(VI) and Pb(II).

Role of mass-kill hunting strategies in the extirpation of Persian gazelle (Gazella subgutturosa) in the northern Levant

PubMed Central

Bar-Oz, Guy; Zeder, Melinda; Hole, Frank

2011-01-01

Continuous and intensive exploitation of wildlife resources by early agricultural societies had major ecological consequences in the ancient Near East. In particular, hunting strategies of post-Neolithic societies involving the mass killing of wild ungulates contributed to the eventual extirpation of a number of wild species. A remarkable deposit of bones of Persian gazelle (Gazella subgutarosa) from fourth millennium BCE levels at Tell Kuran in northeastern Syria provides insight into the unsustainable hunting practices that disrupted gazelle migratory patterns and helped set the course for the virtual extinction of this species and possibly other steppe species in the Levant. The social context of mass kills conducted during periods when people relied primarily on domestic livestock for animal resources sets them apart from the more targeted and sustainable practices of earlier periods, when wild animals were the major or sole source of animal protein. PMID:21502520

Electricity generation from carbon monoxide in a single chamber microbial fuel cell.

PubMed

Mehta, P; Hussain, A; Tartakovsky, B; Neburchilov, V; Raghavan, V; Wang, H; Guiot, S R

2010-05-05

Electricity production from carbon monoxide (CO) is demonstrated in a single chamber microbial fuel cell (MFC) with a CoTMPP-based air cathode. The MFC was inoculated with anaerobic sludge and continuously sparged with CO as a sole carbon source. Volumetric power output was maximized at a CO flow rate of 4.8LLR(-1)d(-1) reaching 6.4mWLR(-1). Several soluble and gaseous degradation products including hydrogen, methane, and acetate were detected, resulting in a relatively low apparent Coulombic efficiency of 8.7%. Tests also demonstrated electricity production from hydrogen and acetate with the highest and fastest increase in voltage exhibited after acetate injection. It is hypothesized that electricity generation in a CO-fed MFC is accomplished by a consortium of carboxydotrophic and carbon monoxide - tolerant anodophilic microorganisms. Crown Copyright © 2010. Published by Elsevier Inc. All rights reserved.

Open-Source web-based geographical information system for health exposure assessment

PubMed Central

2012-01-01

This paper presents the design and development of an open source web-based Geographical Information System allowing users to visualise, customise and interact with spatial data within their web browser. The developed application shows that by using solely Open Source software it was possible to develop a customisable web based GIS application that provides functions necessary to convey health and environmental data to experts and non-experts alike without the requirement of proprietary software. PMID:22233606

A method for separation of heavy metal sources in urban groundwater using multiple lines of evidence.

PubMed

Hepburn, Emily; Northway, Anne; Bekele, Dawit; Liu, Gang-Jun; Currell, Matthew

2018-06-11

Determining sources of heavy metals in soils, sediments and groundwater is important for understanding their fate and transport and mitigating human and environmental exposures. Artificially imported fill, natural sediments and groundwater from 240 ha of reclaimed land at Fishermans Bend in Australia, were analysed for heavy metals and other parameters to determine the relative contributions from different possible sources. Fishermans Bend is Australia's largest urban re-development project, however, complicated land-use history, geology, and multiple contamination sources pose challenges to successful re-development. We developed a method for heavy metal source separation in groundwater using statistical categorisation of the data, analysis of soil leaching values and fill/sediment XRF profiling. The method identified two major sources of heavy metals in groundwater: 1. Point sources from local or up-gradient groundwater contaminated by industrial activities and/or legacy landfills; and 2. contaminated fill, where leaching of Cu, Mn, Pb and Zn was observed. Across the precinct, metals were most commonly sourced from a combination of these sources; however, eight locations indicated at least one metal sourced solely from fill leaching, and 23 locations indicated at least one metal sourced solely from impacted groundwater. Concentrations of heavy metals in groundwater ranged from 0.0001 to 0.003 mg/L (Cd), 0.001-0.1 mg/L (Cr), 0.001-0.2 mg/L (Cu), 0.001-0.5 mg/L (Ni), 0.001-0.01 mg/L (Pb), and 0.005-1.2 mg/L (Zn). Our method can determine the likely contribution of different metal sources to groundwater, helping inform more detailed contamination assessments and precinct-wide management and remediation strategies. Copyright © 2018 Elsevier Ltd. All rights reserved.

Role of Reduced Exogenous Organic Compounds in the Physiology of the Blue-Green Bacteria (Algae): Photoheterotrophic Growth of a “Heterotrophic” Blue-Green Bacterium

PubMed Central

Ingram, L. O.; Calder, J. A.; Van Baalen, C.; Plucker, F. E.; Parker, P. L.

1973-01-01

Nostoc sp. (strain Mac) was shown to be capable of using glucose, fructose, or sucrose as a sole source of carbon and energy in the dark. In the light in the absence of exogenously supplied CO2, this strain exhibited a more versatile metabolism. In addition to the three sugars above, glycerol and acetate served as sole sources of carbon. This photoheterotrophic growth in the absence of exogenously supplied CO2 appears to involve O2-evolving photosynthesis. The action spectrum for photoheterotrophic growth on acetate closely resembles the action spectrum for photosynthesis. The physiology of photoheterotrophic growth was further investigated through determinations of stable carbon isotope ratios and measurements of gas exchanges. These investigations suggest that respired CO2 from substrate oxidation is assimilated by the photosynthetic machinery. PMID:4196252

Microbial Degradation of Isopropyl-N-3-Chlorophenylcarbamate and 2-Chloroethyl-N-3-Chlorophenylcarbamate

PubMed Central

Kaufman, D. D.; Kearney, P. C.

1965-01-01

Microbial degradation of isopropyl-N-3-chlorophenylcarbamate (CIPC) and 2-chloroethyl-N-3-chlorophenylcarbamate (CEPC) was observed in a soil perfusion system. Degradation in perfused soils, and by pure cultures of effective bacterial isolates, was demonstrated by the production of 3-chloroaniline and the subsequent liberation of free chloride ion. Identified isolates effective in degrading and utilizing CIPC as a sole source of carbon included Pseudomonas striata Chester, a Flavobacterium sp., an Agrobacterium sp., and an Achromobacter sp. Identified isolates, effective in degrading and utilizing CEPC as a sole source of carbon, included an Achromobacter sp. and an Arthrobacter sp. CIPC-effective isolates degraded CEPC more slowly than CIPC, whereas CEPC-effective isolates degraded CIPC more rapidly than CEPC. Both CIPC- and CEPC-effective isolates degraded isopropyl N-phenylcarbamate (IPC) more rapidly than either CIPC or CEPC. Images Fig. 3 PMID:14325285

Biodegradation of chlorpyrifos by lactic acid bacteria during kimchi fermentation.

PubMed

Cho, Kye Man; Math, Reukaradhya K; Islam, Shah Md Asraful; Lim, Woo Jin; Hong, Su Young; Kim, Jong Min; Yun, Myoung Geun; Cho, Ji Joong; Yun, Han Dae

2009-03-11

We examined the role of microorganisms in the degradation of the organophosphorus (OP) insecticide chlorpyrifos (CP) during kimchi fermentation. During the fermentation of kimchi, 30 mg L(-1) of CP was added and its stability assayed during fermentation. CP was degraded rapidly until day 3 (83.3%) and degraded completely by day 9. Four CP-degrading lactic acid bacteria (LAB) were isolated from kimchi fermentation in the presence of 200 mg L(-1) CP and were identified as Leuconostoc mesenteroides WCP907, Lactobacillus brevis WCP902, Lactobacillus plantarum WCP931, and Lactobacillus sakei WCP904. CP could be utilized by these four strains as the sole source of carbon and phosphorus. Coumaphos (CM), diazinon (DZ), parathion (PT), and methylparathion (MPT) were also degraded by WCP907, WCP902, WCP931, and WCP904 when provided as sole sources of carbon and phosphorus.

Initial proteome analysis of caffeine-induced proteins in Aspergillus tamarii using two-dimensional fluorescence difference gel electrophoresis.

PubMed

Gutiérrez-Sánchez, Gerardo; Atwood, James; Kolli, V S Kumar; Roussos, Sévastianos; Augur, Christopher

2012-04-01

Caffeine is toxic to most microorganisms. However, some filamentous fungi, such as Aspergillus tamarii, are able to metabolize this alkaloid when fed caffeine as the sole nitrogen source. The aim of the present work was to identify intracellular A. tamarii proteins, regulated by caffeine, using fluorescence difference two-dimensional gel electrophoresis. Specific proteins from two culture media of A. tamarii grown either on ammonium sulfate or caffeine as the sole nitrogen source were analysed by mass spectrometry. Thirteen out of a total of 85 differentially expressed spots were identified after database search. Identified up-regulated proteins include phosphoglycerate kinase, malate dehydrogenase, dyp-type peroxidase family protein, heat shock protein, Cu, Zn superoxidase dismutase and xanthine dehydrogenase. Some of the proteins identified in this study are involved in the caffeine degradation pathway as well as in stress response, suggesting that stress proteins could be involved in caffeine metabolism in filamentous fungi.

Growth of Prototheca isolates on n-hexadecane and mixed-hydrocarbon substrate.

PubMed Central

Walker, J D; Pore, R S

1978-01-01

Prototheca zopfii, an achlorphyllous alga, was capable of using hydrocarbons as sole carbon and energy source. The ability of P. zopfii to use hydrocarbons did not correlate with source of isolation. Seventy-five percent of the P. zopfii cultures recovered from sewage, plants, or animals utilized hydrocarbons. Other Prototheca species and P. zopfii that did not utilize hydrocarbons were isolated simultaneously from several sources with isolates that did use hydrocarbons. Species type rather than source of isolation was the predominant factor that determined hydrocarbon utilization. PMID:565616

Subduction starts by stripping slabs

NASA Astrophysics Data System (ADS)

Soret, Mathieu; Agard, Philippe; Dubacq, Benoît; Prigent, Cécile; Plunder, Alexis; Yamato, Philippe; Guillot, Stéphane

2017-04-01

Metamorphic soles correspond to tectonic slices welded beneath most large-scale ophiolites. These slivers of oceanic crust metamorphosed up to granulite facies conditions are interpreted as having formed during the first My of intra-oceanic subduction from heat transfer from the incipient mantle wedge towards the top of the subducting plate. Our study reappraises the formation of metamorphic sole through detailed field and petrological work on three classical key sections across the Semail ophiolite (Oman and United Arab Emirates). Geothermobarometry and thermodynamic modelling show that metamorphic soles do not record a continuous temperature gradient, as expected from simple heating by the upper plate or by shear heating and proposed by previous studies. The upper, high-temperature metamorphic sole is subdivided in at least two units, testifying to the stepwise formation, detachment and accretion of successive slices from the downgoing slab to the mylonitic base of the ophiolite. Estimated peak pressure-temperature conditions through the metamorphic sole are, from top to bottom, 850˚C - 1GPa, 725°C - 0.8 GPa and 530°C - 0.5 GPa. These estimates appear constant within each unit but separated by a gap of 100 to 200˚C and 0.2 GPa. Despite being separated by hundreds of kilometres below the Semail ophiolite and having contrasting locations with respect to the ophiolite ridge axis, metamorphic soles show no evidence for significant petrological variations along strike. These constraints allow to refine the tectonic-petrological model for the genesis of metamorphic soles, formed through the stepwise stacking of several homogeneous slivers of oceanic crust and its sedimentary cover. Metamorphic soles do not so much result from downward heat transfer (ironing effect) but rather from progressive metamorphism during strain localization and cooling of the plate interface. The successive thrusts are the result of rheological contrasts between the sole (initially at the subducting slab) and the peridotite above as the plate interface progressively cools down. These findings have implications for the thickness, the scale and the coupling state at the plate interface during the early history of subduction/obduction systems.

Potential urban runoff impacts and contaminant distributions in shoreline and reservoir environments of Lake Havasu, southwestern United States.

PubMed

Wilson, Doyle C

2018-04-15

Heavy metal, nutrient, and hydrocarbon levels in and adjacent to Lake Havasu, a regionally significant water supply reservoir with a highly controlled, dynamic flow regime, are assessed in relation to possible stormwater runoff impacts from an arid urban center. Shallow groundwater and sediment analyses from ephemeral drainage (wash) mouths that convey stormwater runoff from Lake Havasu City, Arizona to the reservoir, provided contaminant control points and correlation ties with the reservoir environment. Fine-grain sediments tend to contain higher heavy metal concentrations whereas nutrients are more evenly distributed, except low total organic carbon levels from young wash mouth surfaces devoid of vegetation. Heavy metal and total phosphate sediment concentrations in transects from wash mouths into the reservoir have mixed and decreasing trends, respectively. Both series may indicate chemical depositional influences from urban runoff, yet no statistically significant concentration differences occur between specific wash mouths and corresponding offshore transects. Heavy metal pollution indices of all sediments indicate no discernible to minor contamination, indicating that runoff impacts are minimal. Nevertheless, several heavy metal concentrations from mid-reservoir sediment sites increase southward through the length of the reservoir. Continual significant water flow through the reservoir may help to disperse locally derived runoff particulates, which could mix and settle down gradient with chemical loads from upriver sources and local atmospheric deposition. Incorporating the shoreline environment with the reservoir investigation provides spatial continuity in assessing contaminant sources and distribution patterns. This is particularly acute in the investigation of energetic, flow-through reservoirs in which sources may be overlooked if solely analyzing the reservoir environment. Copyright © 2017 Elsevier B.V. All rights reserved.

Key Enzymes Enabling the Growth of Arthrobacter sp. Strain JBH1 with Nitroglycerin as the Sole Source of Carbon and Nitrogen

PubMed Central

Husserl, Johana; Hughes, Joseph B.

2012-01-01

Flavoprotein reductases that catalyze the transformation of nitroglycerin (NG) to dinitro- or mononitroglycerols enable bacteria containing such enzymes to use NG as the nitrogen source. The inability to use the resulting mononitroglycerols limits most strains to incomplete denitration of NG. Recently, Arthrobacter strain JBH1 was isolated for the ability to grow on NG as the sole source of carbon and nitrogen, but the enzymes and mechanisms involved were not established. Here, the enzymes that enable the Arthrobacter strain to incorporate NG into a productive pathway were identified. Enzyme assays indicated that the transformation of nitroglycerin to mononitroglycerol is NADPH dependent and that the subsequent transformation of mononitroglycerol is ATP dependent. Cloning and heterologous expression revealed that a flavoprotein catalyzes selective denitration of NG to 1-mononitroglycerol (1-MNG) and that 1-MNG is transformed to 1-nitro-3-phosphoglycerol by a glycerol kinase homolog. Phosphorylation of the nitroester intermediate enables the subsequent denitration of 1-MNG in a productive pathway that supports the growth of the isolate and mineralization of NG. PMID:22427495

Key enzymes enabling the growth of Arthrobacter sp. strain JBH1 with nitroglycerin as the sole source of carbon and nitrogen.

PubMed

Husserl, Johana; Hughes, Joseph B; Spain, Jim C

2012-05-01

Flavoprotein reductases that catalyze the transformation of nitroglycerin (NG) to dinitro- or mononitroglycerols enable bacteria containing such enzymes to use NG as the nitrogen source. The inability to use the resulting mononitroglycerols limits most strains to incomplete denitration of NG. Recently, Arthrobacter strain JBH1 was isolated for the ability to grow on NG as the sole source of carbon and nitrogen, but the enzymes and mechanisms involved were not established. Here, the enzymes that enable the Arthrobacter strain to incorporate NG into a productive pathway were identified. Enzyme assays indicated that the transformation of nitroglycerin to mononitroglycerol is NADPH dependent and that the subsequent transformation of mononitroglycerol is ATP dependent. Cloning and heterologous expression revealed that a flavoprotein catalyzes selective denitration of NG to 1-mononitroglycerol (1-MNG) and that 1-MNG is transformed to 1-nitro-3-phosphoglycerol by a glycerol kinase homolog. Phosphorylation of the nitroester intermediate enables the subsequent denitration of 1-MNG in a productive pathway that supports the growth of the isolate and mineralization of NG.

Heterotrophic cultivation of Chlorella pyrenoidosa using sucrose as the sole carbon source by co-culture with Rhodotorula glutinis.

PubMed

Wang, Shikai; Wu, Yong; Wang, Xu

2016-11-01

Heterotrophic cultivation of microalgae is a feasible alternative strategy to avoid the light limitation of photoautotrophic culture, but the heterotrophic utilization of disaccharides is difficult for microalgae. Aimed at this problem, a co-culture system was developed by mix culture of C. pyrenoidosa and R. glutinis using sucrose as the sole carbon source. In this system, C. pyrenoidosa could utilize glucose and fructose which were hydrolyzed from sucrose by R. glutinis. The highest specific growth rate and final cell number proportion of algae was 1.02day(-1) and 45%, respectively, when cultured at the initial algal cell number proportion of 95.24% and the final algal cell density was 111.48×10(6)cells/mL. In addition, the lipid content was also promoted due to the synergistic effects in mix culture. This study provides a novel approach using sucrose-riched wastes for the heterotrophic culture of microalgae and may effectively decrease the cost of carbon source. Copyright © 2016 Elsevier Ltd. All rights reserved.

Functional Characterization of Alanine Racemase from Schizosaccharomyces pombe: a Eucaryotic Counterpart to Bacterial Alanine Racemase

PubMed Central

Uo, Takuma; Yoshimura, Tohru; Tanaka, Naotaka; Takegawa, Kaoru; Esaki, Nobuyoshi

2001-01-01

Schizosaccharomyces pombe has an open reading frame, which we named alr1+, encoding a putative protein similar to bacterial alanine racemase. We cloned the alr1+ gene in Escherichia coli and purified the gene product (Alr1p), with an Mr of 41,590, to homogeneity. Alr1p contains pyridoxal 5′-phosphate as a coenzyme and catalyzes the racemization of alanine with apparent Km and Vmax values as follows: for l-alanine, 5.0 mM and 670 μmol/min/mg, respectively, and for d-alanine, 2.4 mM and 350 μmol/min/mg, respectively. The enzyme is almost specific to alanine, but l-serine and l-2-aminobutyrate are racemized slowly at rates 3.7 and 0.37% of that of l-alanine, respectively. S. pombe uses d-alanine as a sole nitrogen source, but deletion of the alr1+ gene resulted in retarded growth on the same medium. This indicates that S. pombe has catabolic pathways for both enantiomers of alanine and that the pathway for l-alanine coupled with racemization plays a major role in the catabolism of d-alanine. Saccharomyces cerevisiae differs markedly from S. pombe: S. cerevisiae uses l-alanine but not d-alanine as a sole nitrogen source. Moreover, d-alanine is toxic to S. cerevisiae. However, heterologous expression of the alr1+ gene enabled S. cerevisiae to grow efficiently on d-alanine as a sole nitrogen source. The recombinant yeast was relieved from the toxicity of d-alanine. PMID:11244061

Low-threshold, CW, all-solid-state Ti:Al2O3 laser

NASA Technical Reports Server (NTRS)

Harrison, James; Finch, Andrew; Rines, David M.; Rines, Glen A.; Moulton, Peter F.

1991-01-01

A CW Ti:Al2O3 ring laser with a threshold power of 119 mW is demonstrated. It provides a tunable source of single-frequency, diffraction-limited radiation that is suitable for injection seeding. The Ti:Al2O3 laser is operated with a diode-laser-pumped, frequency-doubled, Nd:YAG laser as the sole pump source.

Safe arming system for two-explosive munitions

DOEpatents

Jaroska, Miles F.; Niven, William A.; Morrison, Jasper J.

1978-01-01

A system for safely and positively detonating high-explosive munitions, including a source of electrical signals, a split-phase square-loop transformer responsive solely to a unique series of signals from the source for charging an energy storage circuit through a voltage doubling circuit, and a spark-gap trigger for initiating discharge of the energy in the storage circuit to actuate a detonator and thereby fire the munitions.

The Tell-Tale: What Do Heart Rate; Skin Temperature and Skin Conductance Reveal about Emotions of People with Severe and Profound Intellectual Disabilities?

ERIC Educational Resources Information Center

Vos, Pieter; De Cock, Paul; Munde, Vera; Petry, Katja; Van Den Noortgate, Wim; Maes, Bea

2012-01-01

Identifying emotions in people with severe and profound intellectual disabilities is a difficult challenge. Since self-reports are not available, behaviour is the most used source of information. Given the limitations and caveats associated with using behaviour as the sole source of information about their emotions, it is important to supplement…

7 CFR 4290.210 - Minimum capital requirements for RBICs.

Code of Federal Regulations, 2010 CFR

2010-01-01

..., as determined solely by the Secretary; (ii) Has a viable business plan reasonably projecting... Section 4290.210 Agriculture Regulations of the Department of Agriculture (Continued) RURAL BUSINESS-COOPERATIVE SERVICE AND RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE RURAL BUSINESS INVESTMENT COMPANY...

50 CFR Table 34 to Part 679 - Annual Apportionment of BSAI Yellowfin Sole Between the Amendment 80 and BSAI Trawl Limited...

Code of Federal Regulations, 2010 CFR

2010-10-01

... and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE (CONTINUED) FISHERIES OF THE EXCLUSIVE ECONOMIC ZONE OFF ALASKA Pt. 679, Table 34...

40 CFR 211.110-1 - Testing exemption.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 211.110-1 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS PRODUCT NOISE LABELING General Provisions § 211.110-1 Testing exemption. (a) A new product intended to be used solely for research, investigations, studies, demonstrations or training, and so...

47 CFR 22.503 - Paging geographic area authorizations.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Section 22.503 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES PUBLIC MOBILE SERVICES Paging and Radiotelephone Service § 22.503 Paging geographic area authorizations... in its sole discretion, the FCC determines that the public interest would be served by such...

47 CFR 22.503 - Paging geographic area authorizations.

Code of Federal Regulations, 2014 CFR

2014-10-01

... Section 22.503 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES PUBLIC MOBILE SERVICES Paging and Radiotelephone Service § 22.503 Paging geographic area authorizations... in its sole discretion, the FCC determines that the public interest would be served by such...

47 CFR 22.503 - Paging geographic area authorizations.

Code of Federal Regulations, 2013 CFR

2013-10-01

... Section 22.503 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES PUBLIC MOBILE SERVICES Paging and Radiotelephone Service § 22.503 Paging geographic area authorizations... in its sole discretion, the FCC determines that the public interest would be served by such...

Vegetation component of geothermal EIS studies: Introduced plants, ecosystem stability, and geothermal development

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

This paper contributes new information about the impacts from introduced plant invasions on the native Hawaiian vegetation as consequences of land disturbance and geothermal development activities. In this regard, most geothermal development is expected to act as another recurring source of physical disturbance which favors the spread and maintenance of introduced organisms throughout the region. Where geothermal exploration and development activities extend beyond existing agricultural and residential development, they will become the initial or sole source of disturbance to the naturalized vegetation of the area. Kilauea has a unique ecosystem adapted to the dynamics of a volcanically active landscape. Themore » characteristics of this ecosystem need to be realized in order to understand the major threats to the ecosystem and to evaluate the effects of and mitigation for geothermal development in Puna. The native Puna vegetation is well adapted to disturbances associated with volcanic eruption, but it is ill-adapted to compete with alien plant species in secondary disturbances produced by human activities. Introduced plant and animal species have become a major threat to the continued presence of the native biota in the Puna region of reference.« less

Innovative Approaches to Continuous Professional Development (CPD) in Early Childhood Education and Care (ECEC) in Europe: Findings from a Comparative Review

ERIC Educational Resources Information Center

Jensen, Bente; Iannone, Rosa Lisa

2018-01-01

This article explores innovation as an aspect of in-service continuous professional development (CPD) in ECEC. Based on a literature review and a cross-country analysis conducted in ten European countries, we found that innovation in CPD was understood as a way to improving quality in ECEC. CPD no longer solely deals with practitioners' knowledge…

Optimisation of continuous gas fermentation by immobilisation of acetate-producing Acetobacterium woodii.

PubMed

Steger, Franziska; Rachbauer, Lydia; Windhagauer, Matthias; Montgomery, Lucy F R; Bochmann, Günther

2017-08-01

Hydrogen from water electrolysis is often suggested as a way of storing the excess energy from wind and solar power plants. However, unlike natural gas, hydrogen is difficult to store and distribute. One solution is to convert the hydrogen into other fuels or bulk chemicals. In this study we investigated fermentation in which homoacetogenic clostridia apply the Wood-Ljungdahl pathway to generate acetate from H 2 and CO 2 . Acetate can be used as a bulk chemical or further transformed into biofuels. Autotrophic growth with CO 2 as the sole carbon source is slow compared to heterotrophic growth, so the aim of this work was to improve continuous gas fermentation by immobilising the acetate-producing clostridia, thus preventing their wash out from the bioreactor. Two homoacetogenic bacterial strains (Acetobacterium woodii and Moorella thermoacetica) were tested for their acetate production potential, with A. woodii proving to be the better strain with maximum acetate concentration of 29.57 g l -1 . Due to its stability during fermentation and good bacterial immobilisation, linen was chosen as immobilisation material for continuous fermentation. This study demonstrates the successful continuous fermentation of acetate from H 2 and CO 2 using A. woodii immobilised on a low-cost surface at high volumetric productivity of 1.21 ± 0.05 g acetate l -1 d -1 . This has great industrial potential and future studies should focus on the scale-up of this process. Copyright © 2017 Elsevier Ltd. All rights reserved.

Novel nuclear-cytoplasmic interaction in wheat (Triticum aestivum) induces vigorous plants

USDA-ARS?s Scientific Manuscript database

Interspecific hybridization can be considered an accelerator of evolution, otherwise a slow process, solely dependent on mutation and recombination. Upon interspecific hybridization, several novel interactions between nuclear and cytoplasmic genomes emerge which provide additional sources of diversi...

48 CFR 819.7008 - Sole source awards to veteran-owned small business concerns.

Code of Federal Regulations, 2011 CFR

2011-10-01

... determined to be a responsible contractor with respect to performance; (4) Award can be made at a fair and... businesses are registered and verified as eligible in the VIP database prior to making an award. ...

Acral peeling skin syndrome in two East-African siblings: case report.

PubMed

Kiprono, Samson K; Chaula, Baraka M; Naafs, Bernard; Masenga, John E

2012-03-19

Acral peeling skin syndrome is a rare autosomal recessive genodermatosis due to a missense mutation in transglutaminase 5. The skin peeling occurs at the separation of the stratum corneum from the stratum granulosum. We present a case of two siblings who developed continuous peeling of the palms and soles from the first year of life. This peeling was more severe on the soles than palms and on younger sibling than elder sibling. Peeling is worsened by occlusion and sweating. Sporadic cases of Acral Peeling Skin Syndrome occur in African population. There is variability in time of presentation and clinical severity even within families.

Induction of L-phenylalanine ammonia-lyase during utilization of phenylalanine as a carbon or nitrogen source in Rhodotorula glutinis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Marusich, W.C.; Jensen, R.A.; Zamir, L.O.

Rhodotorula glutinis is a convenient source of L-phenylalanine ammonia-lyase, an enzyme that is useful as a biochemical reagent in the assay of L-phenylalanine. There have been previous descriptions of induced lyase production in complex medium where induction occurs late in exponential growth, suggesting a role in secondary metabolism such as is the case in higher plants. A higher specific activity of L-phenylalanine ammonia-lyase (sixfold higher than in complex medium) can be obtained during midexponential growth in a defined medium containing L-phenylalanine as the sole source of carbon. L-phenylalanine will also induce lyase synthesis during exponential growth in minimal medium inmore » which L-phenylalanine is the sole source of nitrogen. The appearance of lyase in complex medium supplemented with L-phenylalanine is probably triggered fortuitously by exhaustion late in growth of a prime source of nitrogen. In this study, R. glutinis appeared to express a single lyase enzyme, regardless of whether induction was nitrogen signaled or carbon signaled. Thin-layer chromatographic analysis of ether extracts prepared fom cultures induced with doubly labeled (U-/sup 14/C; ring-4-/sup 3/H) L-phenylalanine provided evidence of a catabolic sequence containing cinnamic acid, benzoic acid, and 4-hydroxybenzoic acid as degradative intermediates. 3,4-Dihydroxybenzoic acid was not identified as a catabolic intermediate.« less

Induction of L-phenylalanine ammonia-lyase during utilization of phenylalanine as a carbon or nitrogen source in Rhodotorula glutinis.

PubMed Central

Marusich, W C; Jensen, R A; Zamir, L O

1981-01-01

Rhodotorula glutinis is a convenient source of L-phenylalanine ammonia-lyase, an enzyme that is useful as a biochemical reagent in the assay of L-phenylalanine. There have been previous descriptions of induced lyase production in complex medium where induction occurs late in exponential growth, suggesting a role in secondary metabolism such as is the case in higher plants. A higher specific activity of L-phenylalanine ammonia-lyase (sixfold higher than a complex medium) can be obtained during midexponential growth in a defined medium containing L-phenylalanine as the sole source of carbon. L-Phenylalanine will also induce lyase synthesis during exponential growth in minimal in which L-phenylalanine is the sole source of nitrogen. The appearance of lyase in complex medium supplemented with L-phenylalanine is probably triggered fortuitously by exhaustion late in growth of a prime source of nitrogen. In this study, R. glutinis appeared to express a single lyase enzyme, regardless of whether induction was nitrogen signaled or carbon signaled. Thin-layer chromatographic analysis of ether extracts prepared from cultures induced with doubly labeled (U-14C; ring-4-3H) L-phenylalanine provided evidence of a catabolic sequence containing cinnamic acid, benzoic acid, and 4-hydroxybenzoic acid as degradative intermediates. 3,4-Dihydroxybenzoic acid was not identified as a catabolic intermediate. PMID:7195398

Deformation of allochthonous salt and evolution of related salt-structural systems, eastern Louisiana Gulf Coast

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schuster, D.C.

1996-12-31

Salt tectonics in the northern Gulf of Mexico involves both vertical diapirism and lateral silling or flow of salt into wings and tablets (sheets). Combinations of these two modes of salt deformation, concurrent with sediment loading and salt evacuation, have produced complex structures in the coastal and offshore region of southeastern Louisiana, a prolific oil and gas province. Many large growth faults and salt domes in the study area root into intra-Tertiary salt welds that were formerly occupied by allochthonous salt tablets. Two end-member structural systems involving evacuation of former tabular salt are recognized: roho systems and stepped counter-regional systems.more » Both end-member systems share a similar multi-staged evolution, including (1) initial formation of a south-leaning salt dome or wall sourced from the Jurassic salt level; (2) progressive development into a semi-tabular allochthonous salt body; and (3) subsequent loading, evacuation, and displacement of the tabular salt into secondary domes. In both systems, it is not uncommon to find salt displaced as much as 16-24 km south of its autochthonous source, connected by a horizontal salt weld to an updip, deflated counter-regional feeder. Although both end-member structural systems may originate before loading of allochthonous salt having grossly similar geometry, their final structural configurations after loading and salt withdrawal are distinctly different. Roho systems are characterized by large-displacement, listric, south-dipping growth faults that sole into intra-Tertiary salt welds marked by high-amplitude reflections continuous with residual salt masses. Salt from the former salt tablets has been loaded and squeezed laterally and downdip. Stepped counter-regional systems, in contrast, comprise large salt domes and adjacent large-displacement, north-dipping growth faults that sole into intra-Tertiary salt welds before stepping down again farther north.« less

Isolation and characterization of sulphur-oxidizing Thiomonas sp. and its potential application in biological deodorization.

PubMed

Chen, X-G; Geng, A-L; Yan, R; Gould, W D; Ng, Y-L; Liang, D T

2004-01-01

To isolate and characterize a sulphur-oxidizing bacterial strain from activated sludge and to evaluate its potential application in biological deodorization. A dominant sulphur-oxidizing bacterial strain, designated as strain SS, was isolated from an enrichment culture using thiosulphate as a sole energy source and CO2 as a sole carbon source. The cells of this organism were aerobic, rod-shaped, Gram-negative and motile. Strain SS could grow autotrophically, heterotrophically as well as mixotrophically. Autotrophic growth was observed at pH values ranging from 2.3 to 9.0. Phylogenetic analyses revealed that strain SS belonged to Group 1 of the genus Thiomonas, closely related to Thiomonas perometabolis and Thiomonas intermedia. The thiosulphate oxidation rates of strain SS at different pH values were evaluated in terms of oxygen uptake using a Micro-Oxymax respirometer. The results showed that the maximum oxidation rate of 5.65 mg l(-1) h(-1) occurred at 56 h of growth and pH 6.0. Continuous H2S removal study demonstrated that strain SS could remove more than 99% of H2S when the inlet concentration was below 58.6 ppm. Further increase of the inlet concentration to 118 ppm gave rise to a decline in the removal efficiency to ca 90%. The strong acidification of the culture medium during the later period could result in the deterioration of the growth activity and the metabolism activity of strain SS. In practical application, the problems caused by the end-product inhibition and the acidification can be alleviated by periodical replacement of culture medium with fresh medium. Given the physiological flexibility and the ability to remove H2S rapidly and efficiently, strain SS could be a good 'deodorizing' candidate. This is the first time that Thiomonas species has been reported for biological deodorization application.

Poly-β-hydroxybutyrate and exopolysaccharide biosynthesis by bacterial isolates from pigeonpea [Cajanus cajan (L.) Millsp] root nodules.

PubMed

Fernandes, Paulo Ivan; de Oliveira, Paulo Jansen; Rumjanek, Norma Gouvêa; Xavier, Gustavo Ribeiro

2011-02-01

The bacterial strains that are able to produce biopolymers that are applied in industrial sectors present a source of renewable resources. Some microorganisms are already applied at several industrial sectors, but the prospecting of new microbes must bring microorganisms that are feasible to produce interesting biopolymers more efficiently and in cheaper conditions. Among the biopolymers applied industrially, polyhydroxybutyrate (PHB) and exopolysaccharides (EPS) stand out because of its applications, mainly in biodegradable plastic production and in food industry, respectively. In this context, the capacity of bacteria isolated from pigeonpea root nodules to produce EPS and PHB was evaluated, as well as the cultural characterization of these isolates. Among the 38 isolates evaluated, the majority presented fast growth and ability to acidify the culture media. Regarding the biopolymer production, five isolates produced more than 10 mg PHB per liter of culture medium. Six EPS producing bacteria achieved more than 200 mg EPS per liter of culture medium. Evaluating different carbon sources, the PHB productivity of the isolate 24.6b reached 69% of cell dry weight when cultured with starch as sole carbon source, and the isolate 8.1c synthesized 53% PHB in dry cell biomass and more than 1.3 g L⁻¹ of EPS when grown using xylose as sole carbon source.

7 CFR 3565.455 - Alternative disposition methods.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 15 2011-01-01 2011-01-01 false Alternative disposition methods. 3565.455 Section 3565.455 Agriculture Regulations of the Department of Agriculture (Continued) RURAL HOUSING SERVICE....455 Alternative disposition methods. The Agency, in its sole discretion, may choose to obtain an...

5 CFR 831.642 - Marriage duration requirements.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 5 Administrative Personnel 2 2014-01-01 2014-01-01 false Marriage duration requirements. 831.642 Section 831.642 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT (CONTINUED) CIVIL SERVICE... infection of and through a visible wound sustained solely through violent, external, and accidental means...

5 CFR 831.642 - Marriage duration requirements.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 5 Administrative Personnel 2 2013-01-01 2013-01-01 false Marriage duration requirements. 831.642 Section 831.642 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT (CONTINUED) CIVIL SERVICE... infection of and through a visible wound sustained solely through violent, external, and accidental means...

5 CFR 831.642 - Marriage duration requirements.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 5 Administrative Personnel 2 2011-01-01 2011-01-01 false Marriage duration requirements. 831.642 Section 831.642 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT (CONTINUED) CIVIL SERVICE... infection of and through a visible wound sustained solely through violent, external, and accidental means...

5 CFR 831.642 - Marriage duration requirements.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 5 Administrative Personnel 2 2012-01-01 2012-01-01 false Marriage duration requirements. 831.642 Section 831.642 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT (CONTINUED) CIVIL SERVICE... infection of and through a visible wound sustained solely through violent, external, and accidental means...

Low external pH replaces 2,4-D in maintaining and multiplying 2,4-D-initiated embryogenic cells of carrot

NASA Technical Reports Server (NTRS)

Smith, D. L.; Krikorian, A. D.

1990-01-01

A mixed culture comprised of both embryonic globules and nonembryogenic callus was derived from seedling hypocotyls of Daucus carota cv. Scarlet Nantes on 2,4-D- containing medium using well-established methods. Then the mixed cultures were transferred to, and serially subcultured on, a hormone-free medium near pH 4. The medium contained 1 mM NH4+ as the sole nitrogen source. When cultured in this way, embryonic globules were able to multiply without development into later embryo stages. Nonembryogenic callus did not survive. Continuous culture of embryonic globules on this low pH hormone-free medium yielded cultures consisting entirely of preglobular stage proembryos (PGSPs). PGSP cultures have been maintained as such with continuous multiplication for nearly 2 years without loss of embryogenic potential. These hormone-free-maintained PGSPs continue their development to later embryo stages when cultured on the same hormone-free medium buffered at pH 5.8. We show that hormone-free medium near pH 4 can replace 2,4-D in its ability to sustain multiplication of 2,4-D-initiated embryogenic cells of carrot at an acceptable growth rate without their development into later embryo stages. This procedure provides selective conditions that do not permit the growth of non-embryogenic cells while providing an adequate environment for embryogenic cell proliferation and should prove invaluable in studying habituation.

RELATIONSHIPS OF NATURAL ENEMIES AND NON-PREY FOODS

USDA-ARS?s Scientific Manuscript database

There are very few entomophagous species so maladapted as to rely on prey as their sole nutritional resource. Although a rich source of nutrients, prey/host availability to predators and parasitoids is restricted temporally by ephemeral population dynamics, spatial differences in microclimate, struc...

OBSERVATIONS FROM CONTAMINANT PLUMES ON LONG ISLAND

EPA Science Inventory

The aquifers of Long Island serve as a sole source drinking water supply for the entire
local population of about three million people. Where the shallow Upper Glacial Aquifer has been contaminated with petroleum hydrocarbons and methyl tert-butyl ether (MTBE), intensive site ...

Effect of varying dietary levels of LC-PUFA and vegetable oil sources on performance and fatty acids of Senegalese sole post larvae: puzzling results suggest complete biosynthesis pathway from C18 PUFA to DHA.

PubMed

Navarro-Guillén, Carmen; Engrola, Sofia; Castanheira, Filipa; Bandarra, Narcisa; Hachero-Cruzado, Ismael; Tocher, Douglas R; Conceição, Luís E C; Morais, Sofia

2014-01-01

Lipid nutrition of marine fish larvae has focused on supplying essential fatty acids (EFA) at high levels to meet requirements for survival, growth and development. However, some deleterious effects have been reported suggesting that excessive supply of EFA might result in insufficient supply of energy substrates, particularly in species with lower EFA requirements such as Senegalese sole (Solea senegalensis). This study addressed how the balance between EFA and non-EFA (better energy sources) affects larval performance, body composition and metabolism and retention of DHA, by formulating enrichment emulsions containing two different vegetable oil sources (olive oil or soybean oil) and three DHA levels. DHA positively affected growth and survival, independent of oil source, confirming that for sole post-larvae it is advantageous to base enrichments on vegetable oils supplying higher levels of energy, and supplement these with a DHA-rich oil. In addition, body DHA levels were generally comparable considering the large differences in their dietary supply, suggesting that the previously reported ∆4 fatty acyl desaturase (fad) operates in vivo and that DHA was synthesized at physiologically significant rates through a mechanism involving transcriptional up-regulation of ∆4fad, which was significantly up-regulated in the low DHA treatments. Furthermore, data suggested that DHA biosynthesis may be regulated by an interaction between dietary n-3 and n-6 PUFA, as well as by levels of LC-PUFA, and this may, under certain nutritional conditions, lead to DHA production from C18 precursors. The molecular basis of putative fatty acyl ∆5 and ∆6 desaturation activities remains to be fully determined as thorough searches have found only a single (∆4) Fads2-type transcript. Therefore, further studies are required but this might represent a unique activity described within vertebrate fads. © 2013.

Generation and Phenotypic Characterization of Aspergillus nidulans Methylisocitrate Lyase Deletion Mutants: Methylisocitrate Inhibits Growth and Conidiation

PubMed Central

Brock, Matthias

2005-01-01

Propionate is a very abundant carbon source in soil, and many microorganisms are able to use this as the sole carbon source. Nevertheless, propionate not only serves as a carbon source for filamentous fungi but also acts as a preservative when added to glucose containing media. To solve this contradiction between carbon source and preservative effect, propionate metabolism of Aspergillus nidulans was studied and revealed the methylcitrate cycle as the responsible pathway. Methylisocitrate lyase is one of the key enzymes of that cycle. It catalyzes the cleavage of methylisocitrate into succinate and pyruvate and completes the α-oxidation of propionate. Previously, methylisocitrate lyase was shown to be highly specific for the substrate (2R,3S)-2-methylisocitrate. Here, the identification of the genomic sequence of the corresponding gene and the generation of deletion mutants is reported. Deletion mutants did not grow on propionate as sole carbon and energy source and were severely inhibited during growth on alternative carbon sources, when propionate was present. The strongest inhibitory effect was observed, when glycerol was the main carbon source, followed by glucose and acetate. In addition, asexual conidiation was strongly impaired in the presence of propionate. These effects might be caused by competitive inhibition of the NADP-dependent isocitrate dehydrogenase, because the Ki of (2R,3S)-2-methylisocitrate, the product of the methylcitrate cycle, on NADP-dependent isocitrate dehydrogenase was determined as 1.55 μM. Other isomers had no effect on enzymatic activity. Therefore, methylisocitrate was identified as a potential toxic compound for cellular metabolism. PMID:16151139

Childhood bereavement services: a survey of UK provision.

PubMed

Rolls, L; Payne, S

2003-07-01

The purpose of the study was to identify the location, range and type of childhood bereavement service provision in the UK. A questionnaire was mailed to 127 services who were either solely dedicated to childhood bereavement or who offered a service within the range of work of a host organization and for which there was a supporting organizational structure. Responses were received from 108 services (a response rate of 85%). The findings identified that 85% of childhood bereavement services are located in the voluntary sector; 14% are dedicated childhood bereavement services, while 86% are offered as part of a host organization. Forty-four per cent of host organizations are hospices. The majority of services (73%) relied on both paid and unpaid staff, with 11% relying entirely on paid staff and 14% of services relying entirely on unpaid staff. The interventions offered ranged from individual family work (86%), individual child work (62%), groupwork with families (53%) and groupwork with children (45%). In addition, services offered prebereavement support (64%), a 'drop-in' service (17%), information and advice (95%), training (32%) and the provision of resources (88%). As well as offering a service to children and their families, 74% of childhood bereavement services provided a service to 'secondary users', such as schools (66%), the emergency services (28%) and other professionals (63%). In terms of funding, 12% of services relied solely on external sources of funding, including donations, legacies, revenue from the host organization or grants, while 12% of services relied solely on internal sources of funding, including fundraising and training. The majority of services (73%), however, gained income from a range of sources. The study identifies the diversity of provision that has implications for the evaluation of childhood bereavement services.

Two pectin lyase genes, pnl-1 and pnl-2, from Colletotrichum gloeosporioides f. sp. malvae differ in a cellulose-binding domain and in their expression during infection of Malva pusilla.

PubMed

Wei, Yangdou; Shih, Jenny; Li, Jieran; Goodwin, Paul H

2002-07-01

Two pectin lyase genes, designated pnl-1 and pnl-2, were cloned from Colletotrichum gloeosporioides f. sp. malvae, a pathogen of round-leaved mallow (Malva pusilla). pnl-1 was isolated using cDNA from infected plant material; pnl-2 was isolated using cDNA from 3-day-old mycelia grown in mallow-cell-wall extract (MCWE) broth. pnl-1 is the first pectinase gene described thus far to encode a cellulose-binding domain (CBD), which is common in cellulases and xylanases, whereas pnl-2 encodes a pectin lyase that lacks a CBD. In pure culture, pnl-1 expression could be detected when purified pectin or glucose was the sole carbon source, but not when MCWE was the sole carbon source. The lack of pnl-1 expression appeared to be due to gene repression by some unknown factor(s) in the cell-wall extract. In contrast, expression of pnl-2 was detected in cultures when MCWE, but not when purified pectin or glucose, was the sole carbon source. In infected tissue, detection of pnl-1 expression by Northern-blot hybridization and by RT-PCR began with the onset of the necrotrophic phase of infection. Expression ofpnl-2 was not detectable by Northern-blot hybridization, but was observed byRT-PCR in both the biotrophic and necrotrophic phases of infection. The differences between pnl-1 and pnl-2 (i.e. pnl-1 encoding a CBD and differences in the expression patterns of both genes) may be related to the requirements of C. gloeosporioides f. sp. malvae to be able to grow in host tissue under the different conditions present during the biotrophic and necrotrophic phases of infection.

Biodegradation of the Organic Disulfide 4,4′-Dithiodibutyric Acid by Rhodococcus spp.

PubMed Central

Khairy, Heba; Wübbeler, Jan Hendrik

2015-01-01

Four Rhodococcus spp. exhibited the ability to use 4,4′-dithiodibutyric acid (DTDB) as a sole carbon source for growth. The most important step for the production of a novel polythioester (PTE) using DTDB as a precursor substrate is the initial cleavage of DTDB. Thus, identification of the enzyme responsible for this step was mandatory. Because Rhodococcus erythropolis strain MI2 serves as a model organism for elucidation of the biodegradation of DTDB, it was used to identify the genes encoding the enzymes involved in DTDB utilization. To identify these genes, transposon mutagenesis of R. erythropolis MI2 was carried out using transposon pTNR-TA. Among 3,261 mutants screened, 8 showed no growth with DTDB as the sole carbon source. In five mutants, the insertion locus was mapped either within a gene coding for a polysaccharide deacetyltransferase, a putative ATPase, or an acetyl coenzyme A transferase, 1 bp upstream of a gene coding for a putative methylase, or 176 bp downstream of a gene coding for a putative kinase. In another mutant, the insertion was localized between genes encoding a putative transcriptional regulator of the TetR family (noxR) and an NADH:flavin oxidoreductase (nox). Moreover, in two other mutants, the insertion loci were mapped within a gene encoding a hypothetical protein in the vicinity of noxR and nox. The interruption mutant generated, R. erythropolis MI2 noxΩtsr, was unable to grow with DTDB as the sole carbon source. Subsequently, nox was overexpressed and purified, and its activity with DTDB was measured. The specific enzyme activity of Nox amounted to 1.2 ± 0.15 U/mg. Therefore, we propose that Nox is responsible for the initial cleavage of DTDB into 2 molecules of 4-mercaptobutyric acid (4MB). PMID:26407888

47 CFR 80.880 - Vessel radio equipment.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 5 2010-10-01 2010-10-01 false Vessel radio equipment. 80.880 Section 80.880 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES STATIONS IN THE... Vessel radio equipment. (a) Vessels operated solely within twenty nautical miles of shore must be...

40 CFR 152.6 - Substances excluded from regulation by FIFRA.

Code of Federal Regulations, 2012 CFR

2012-07-01

... FIFRA. 152.6 Section 152.6 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... from FIFRA regulation if they meet the specified conditions or criteria. (a) Liquid chemical sterilants... urease production through action affecting soil bacteria and is distributed and sold solely for those...

40 CFR 152.6 - Substances excluded from regulation by FIFRA.

Code of Federal Regulations, 2014 CFR

2014-07-01

... FIFRA. 152.6 Section 152.6 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... from FIFRA regulation if they meet the specified conditions or criteria. (a) Liquid chemical sterilants... urease production through action affecting soil bacteria and is distributed and sold solely for those...

40 CFR 152.6 - Substances excluded from regulation by FIFRA.

Code of Federal Regulations, 2013 CFR

2013-07-01

... FIFRA. 152.6 Section 152.6 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... from FIFRA regulation if they meet the specified conditions or criteria. (a) Liquid chemical sterilants... urease production through action affecting soil bacteria and is distributed and sold solely for those...

24 CFR 242.67 - New corporations, subsidiaries, affiliations, and mergers.

Code of Federal Regulations, 2012 CFR

2012-04-01

... and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING.... The mortgagor shall not establish, develop, organize, acquire, become the sole member of, or acquire an interest sufficient to require disclosure on the audited financial statements of the mortgagor, in...

24 CFR 242.67 - New corporations, subsidiaries, affiliations, and mergers.

Code of Federal Regulations, 2011 CFR

2011-04-01

... and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING.... The mortgagor shall not establish, develop, organize, acquire, become the sole member of, or acquire an interest sufficient to require disclosure on the audited financial statements of the mortgagor, in...

24 CFR 242.67 - New corporations, subsidiaries, affiliations, and mergers.

Code of Federal Regulations, 2013 CFR

2013-04-01

... and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING.... The mortgagor shall not establish, develop, organize, acquire, become the sole member of, or acquire an interest sufficient to require disclosure on the audited financial statements of the mortgagor, in...

38 CFR 60.8 - Lodging availability.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 38 Pensions, Bonuses, and Veterans' Relief 2 2010-07-01 2010-07-01 false Lodging availability. 60.8 Section 60.8 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS (CONTINUED) FISHER HOUSES AND OTHER TEMPORARY LODGING § 60.8 Lodging availability. Fisher Houses are available solely...

38 CFR 60.8 - Lodging availability.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 38 Pensions, Bonuses, and Veterans' Relief 2 2011-07-01 2011-07-01 false Lodging availability. 60.8 Section 60.8 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS (CONTINUED) FISHER HOUSES AND OTHER TEMPORARY LODGING § 60.8 Lodging availability. Fisher Houses are available solely...

29 CFR 1641.5 - Processing of complaints filed with OFCCP.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 1641.5 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION... act as EEOC's agent for the sole purposes of receiving, investigating and processing the ADA charge... work-sharing agreements between EEOC and State and local agencies designated as FEP agencies, the...

29 CFR 1641.5 - Processing of complaints filed with OFCCP.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 1641.5 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION... act as EEOC's agent for the sole purposes of receiving, investigating and processing the ADA charge... work-sharing agreements between EEOC and State and local agencies designated as FEP agencies, the...

29 CFR 1641.5 - Processing of complaints filed with OFCCP.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 1641.5 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION... act as EEOC's agent for the sole purposes of receiving, investigating and processing the ADA charge... work-sharing agreements between EEOC and State and local agencies designated as FEP agencies, the...

29 CFR 1641.5 - Processing of complaints filed with OFCCP.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 1641.5 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION... act as EEOC's agent for the sole purposes of receiving, investigating and processing the ADA charge... work-sharing agreements between EEOC and State and local agencies designated as FEP agencies, the...

29 CFR 1641.5 - Processing of complaints filed with OFCCP.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 1641.5 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION... act as EEOC's agent for the sole purposes of receiving, investigating and processing the ADA charge... work-sharing agreements between EEOC and State and local agencies designated as FEP agencies, the...

Electrolytic photodissociation of chemical compounds by iron oxide electrodes

DOEpatents

Somorjai, Gabor A.; Leygraf, Christofer H.

1984-01-01

Chemical compounds can be dissociated by contacting the same with a p/n type semi-conductor diode having visible light as its sole source of energy. The diode consists of low cost, readily available materials, specifically polycrystalline iron oxide doped with silicon in the case of the n-type semi-conductor electrode, and polycrystalline iron oxide doped with magnesium in the case of the p-type electrode. So long as the light source has an energy greater than 2.2 electron volts, no added energy source is needed to achieve dissociation.

Electrolytic photodissociation of chemical compounds by iron oxide photochemical diodes

DOEpatents

Somorjai, Gabor A.; Leygraf, Christofer H.

1985-01-01

Chemical compounds can be dissociated by contacting the same with a p/n type semi-conductor photochemical diode having visible light as its sole source of energy. The photochemical diode consists of low cost, readily available materials, specifically polycrystalline iron oxide doped with silicon in the case of the n-type semi-conductor electrode, and polycrystalline iron oxide doped with magnesium in the case of the p-type electrode. So long as the light source has an energy greater than 2.2 electron volts, no added energy source is needed to achieve dissociation.

Reduction of blood serum cholesterol

NASA Technical Reports Server (NTRS)

Winitz, M. (Inventor)

1974-01-01

By feeding a human subject as the sole source of sustenance a defined diet wherein the carbohydrate consists substantially entirely of glucose, maltose or a polysaccharide of glucose, the blood serum cholesterol level of the human subject is substantially reduced. If 25 percent of the carbohydrate is subsequently supplied in the form of sucrose, an immediate increase from the reduced level is observed. The remainder of the defined diet normally includes a source of amino acids, such as protein or a protein hydrolysate, vitamins, minerals and a source of essential fatty acid.

Enrichments of methanotrophic-heterotrophic cultures with high poly-β-hydroxybutyrate (PHB) accumulation capacities.

PubMed

Zhang, Tingting; Wang, Xiaowei; Zhou, Jiti; Zhang, Yu

2018-03-01

Methanotrophic-heterotrophic communities were selectively enriched from sewage sludge to obtain a mixed culture with high levels of poly-β-hydroxybutyrate (PHB) accumulation capacity from methane. Methane was used as the carbon source, N 2 as sole nitrogen source, and oxygen and Cu content were varied. Copper proved essential for PHB synthesis. All cultures enriched with Cu could accumulate high content of PHB (43.2%-45.9%), while only small amounts of PHB were accumulated by cultures enriched without Cu (11.9%-17.5%). Batch assays revealed that communities grown with Cu and a higher O 2 content synthesized more PHB, which had a wider optimal CH 4 :O 2 range and produced a high PHB content (48.7%) even though in the presence of N 2 . In all methanotrophic-heterotrophic communities, both methanotrophic and heterotrophic populations showed the ability to accumulate PHB. Although methane was added as the sole carbon source, heterotrophs dominated with abundances between 77.2% and 85.6%. All methanotrophs detected belonged to type II genera, which formed stable communities with heterotrophs of different PHB production capacities. Copyright © 2017. Published by Elsevier B.V.

Isolation of Bacteria Capable of Growth with 2-Methylisoborneol and Geosmin as the Sole Carbon and Energy Sources

PubMed Central

Guttman, Lior

2012-01-01

Using a relatively simple enrichment technique, geosmin and 2-methylisoborneol (MIB)-biodegrading bacteria were isolated from a digestion basin in an aquaculture unit. Comparison of 16S rRNA gene sequences affiliated one of the three isolates with the Gram-positive genus Rhodococcus, while the other two isolates were found to be closely related to the Gram-negative family Comamonadaceae (Variovorax and Comamonas). Growth rates and geosmin and MIB removal rates by the isolates were determined under aerated and nonaerated conditions in mineral medium containing either of the two compounds as the sole carbon and energy source. All isolates exhibited their fastest growth under aerobic conditions, with generation times ranging from 3.1 to 5.7 h, compared to generation times of up to 19.1 h in the nonaerated flasks. Incubation of the isolates with additional carbon sources caused a significant increase in their growth rates, while removal rates of geosmin and MIB were significantly lower than those for incubation with only geosmin or MIB. By fluorescence in situ hybridization, members of the genera Rhodococcus and Comamonas were detected in geosmin- and MIB-enriched sludge from the digestion basin. PMID:22081577

Management of Electronic Test Equipment. Volume 4. DoD Policy.

DTIC Science & Technology

1986-07-01

sources of supply , the PIL should not mandate sole source dependenc . but rather limit the variety to a minimum of two items. To clarify the controversy...equipment/ supplies on-hand, equipment readiness, and training. The resource area C-ratings are based on stated criteria. The criteria for equipment...respectively). The UNITREP leaves it up to the Military Services whether to include test equipment in the equipment/ supplies on-hand resource area. Although

Lipid formation and γ-linolenic acid production by Mucor circinelloides and Rhizopus sp., grown on vegetable oil

PubMed Central

Tauk-Tornisielo, Sâmia M.; Arasato, Luciana S.; de Almeida, Alex F.; Govone, José S.; Malagutti, Eleni N.

2009-01-01

The fungi strains were tested in Bioscreen automated system to select the best nutritional source. Following, shaking submserse cultures were studied in media containing sole carbon or nitrogen source. The growth of these strains improved in media containing vegetable oil, with high concentration of lipids. The high concentration of γ-linolenic acid was obtained with M. circinelloides in culture containing sesame oil. PMID:24031370

Formation and Fate of Bacterial Sulfonates

DTIC Science & Technology

1989-01-05

sulfonates under phototrophic, anaerobic conditions’ Three cyanobacteria--a strain each of Synechococcus, Anabena, and Nostoc --have been examined for...their ability to utilize the sulfonate taurine as sole source of S for their oxygenic phototrophic growth; only Anabena and Nostoc were able to do so, and

COMPARATIVE EVALUATION OF MTBE SITES ON LONG ISLAND

EPA Science Inventory

The aquifers of Long Island serve as the sole-source drinking water supply for approximately 3 million people. About 20 percent of this population obtains drinking water directly from the water table aquifer (Upper Glacial) whereas the remainder obtain their drinking water from d...

77 FR 23369 - Federal Acquisition Regulation; Justification and Approval of Sole-Source 8(a) Contracts

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-18

... DEPARTMENT OF DEFENSE GENERAL SERVICES ADMINISTRATION NATIONAL AERONAUTICS AND SPACE... AGENCY: Department of Defense (DoD), General Services Administration (GSA), and National Aeronautics and... Department of Defense, the General Services Administration, and the National Aeronautics and Space...

15 CFR 23.3 - Plan.

Code of Federal Regulations, 2010 CFR

2010-01-01

... children information in domestic penalty mail directed to the public and Federal employees. (b) The... newsletters, bulletins, etc. (d) Missing children information will not be placed on letter-size envelopes in... Children (National Center) will be the sole source from which the Department of Commerce will obtain the...

40 CFR 149.101 - Definitions.

Code of Federal Regulations, 2010 CFR

2010-07-01

... AQUIFERS Review of Projects Affecting the Edwards Underground Reservoir, A Designated Sole Source Aquifer... specifically provided, the term(s): (a) Act means the Public Health Service Act, as amended by the Safe Drinking Water Act, Public Law 93-523. (b) Contaminant means any physical, chemical, biological, or...

21 CFR 558.600 - Tiamulin.

Code of Federal Regulations, 2011 CFR

2011-04-01

... treated with tiamulin should not have access to feeds containing polyether ionophores (e.g., lasalocid... not been determined. (3) Use as sole source of tiamulin. (e) Conditions of use—(1) Swine. It is used... bacterial enteritis caused by Escherichia coli and Salmonella choleraesuis and bacterial pneumonia caused by...

Sports Facilities, Zapopan, Jalisco, Mexico.

ERIC Educational Resources Information Center

Amelar, Sarah

2001-01-01

Highlights a new K-12 school gymnasium in Mexico that changes and reacts to weather conditions, requires no air conditioning, and, on typical days, uses sunlight filtering through its ample clerestory as the sole source of illumination. Includes numerous photographs, a section drawing, and a site plan. (GR)

46 CFR 112.35-1 - General.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Manually Controlled Emergency Systems Having a Storage Battery or a Diesel Engine or Gas Turbine Driven Generator as the Sole Emergency Power Source § 112.35-1 General. This subpart contains requirements applicable to emergency power installations having a manually controlled storage battery, diesel engine, or...

46 CFR 112.35-1 - General.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Manually Controlled Emergency Systems Having a Storage Battery or a Diesel Engine or Gas Turbine Driven Generator as the Sole Emergency Power Source § 112.35-1 General. This subpart contains requirements applicable to emergency power installations having a manually controlled storage battery, diesel engine, or...

Characterization of medium-chain-length polyhydroxyalkanoate biosynthesis by Pseudomonas mosselii TO7 using crude glycerol.

PubMed

Liu, Ming-Hsu; Chen, Yi-Jr; Lee, Chia-Yin

2018-03-01

Polyhydroxyalkanoates (PHAs) are biopolyesters produced by microorganisms that are environmentally friendly. PHAs can be used to replace traditional plastic to reduce environmental pollution in various fields. PHA production costs are high because PHA must be produced from a carbon substrate. The purpose of this study was to find the strain that can used the BDF by-product as the sole carbon source to produce high amounts of medium-chain-length PHA. Three isolates were evaluated for potential PHA production by using biodiesel-derived crude glycerol as the sole carbon source. Among them, Pseudomonas mosselii TO7 yielded high PHA content. The PHA produced from P. mosselii TO7 were medium-chain-length-PHAs. The PHA content of 48% cell dry weight in 48 h with a maximum PHA productivity of 13.16 mg PHAs L -1  h -1 . The narrow polydispersity index value of 1.3 reflected the homogeneity of the polymer chain, which was conducive to industrial applications.

Genetic Analysis of Benzothiophene Biodesulfurization Pathway of Gordonia terrae Strain C-6

PubMed Central

Lian, Kehui; Zhang, Yue; Tian, Huimei; Ji, Kaihua; Li, Guoqiang

2013-01-01

Sulfur can be removed from benzothiophene (BT) by some bacteria without breaking carbon-carbon bonds. However, a clear mechanism for BT desulfurization and its genetic components have not been reported in literatures so far. In this study, we used comparative transcriptomics to study differential expression of genes in Gordonia terrae C-6 cultured with BT or sodium sulfate as the sole source of sulfur. We found that 135 genes were up-regulated with BT relative to sodium sulfate as the sole sulfur source. Many of these genes encode flavin-dependent monooxygenases, alkane sulfonate monooxygenases and desulfinase, which perform similar functions to those involved in the 4S pathway of dibenzothiophene (DBT) biodesulfurization. Three of the genes were found to be located in the same operon, designated bdsABC. Cell extracts of pET28a-bdsABC transfected E. coli Rosetta (DE3) converted BT to a phenolic compound, identified as o-hydroxystyrene. These results advance our understanding of enzymes involved in the BT biodesulfurization pathway. PMID:24367657

Phoma herbarum, a soil fungus able to grow on natural lignin and synthetic lignin (DHP) as sole carbon source and cause lignin degradation.

PubMed

Bi, Ran; Lawoko, Martin; Henriksson, Gunnar

2016-08-01

The fungus Phoma herbarum isolated from soil showed growth on highly pure lignin extracted from spruce wood and on synthetic lignin (DHP). The lignin remaining after cultivation was shown to have a lower molecular weight. The reduction in the numbers of ether linkages of the extracted lignins was also observed by derivatization followed by reductive cleavage (DFRC) in combination with (31)P NMR studies. The fungal strain showed an ability to degrade synthetic lignin by extracellular catalysts. GC-MS was applied to study the evolution of low molar mass adducts, e.g., monolignols and it was shown that a reduced coniferyl alcohol product was produced from DHP in a cell-free environment. The work has demonstrated the ability of soil microbes to grow on lignin as sole carbon source. The potential impact is in the production of low molar mass renewable phenols for material application.

Financial Impact of Dual Vendor, Matrix Pricing, and Sole-Source Contracting on Implant Costs.

PubMed

Althausen, Peter L; Lapham, Joan; Mead, Lisa

2016-12-01

Implant costs comprise the largest proportion of operating room supply costs for orthopedic trauma care. Over the years, hospitals have devised several methods of controlling these costs with the help of physicians. With increasing economic pressure, these negotiations have a tremendous ability to decrease the cost of trauma care. In the past, physicians have taken no responsibility for implant pricing which has made cost control difficult. The reasons have been multifactorial. However, industry surgeon consulting fees, research support, and surgeon comfort with certain implant systems have played a large role in slowing adoption of cost-control measures. With the advent of physician gainsharing and comanagement agreements, physicians now have impetus to change. At our facility, we have used 3 methods for cost containment since 2009: dual vendor, matrix pricing, and sole-source contracting. Each has been increasingly successful, resulting in massive savings for the institution. This article describes the process and benefits of each model.

Characterization and Genomic Analysis of a Highly Efficient Dibutyl Phthalate-Degrading Bacterium Gordonia sp. Strain QH-12.

PubMed

Jin, Decai; Kong, Xiao; Liu, Huijun; Wang, Xinxin; Deng, Ye; Jia, Minghong; Yu, Xiangyang

2016-06-25

A bacterial strain QH-12 isolated from activated sludge was identified as Gordonia sp. based on analysis of 16S rRNA gene sequence and was found to be capable of utilizing dibutyl phthalate (DBP) and other common phthalate esters (PAEs) as the sole carbon and energy source. The degradation kinetics of DBP under different concentrations by the strain QH-12 fit well with the modified Gompertz model (R² > 0.98). However, strain QH-12 could not utilize the major intermediate product phthalate (phthalic acid; PA) as the sole carbon and energy source, and only a little amount of PA was detected. The QH-12 genome analysis revealed the presence of putative hydrolase/esterase genes involved in PAEs-degradation but no phthalic acid catabolic gene cluster was found, suggesting that a novel degradation pathway of PAEs was present in Gordonia sp. QH-12. This information will be valuable for obtaining a more holistic understanding on diverse genetic mechanisms of PAEs-degrading Gordonia sp. strains.

Two-dimensional isobutyl acetate production pathways to improve carbon yield

PubMed Central

Tashiro, Yohei; Desai, Shuchi H.; Atsumi, Shota

2015-01-01

For an economically competitive biological process, achieving high carbon yield of a target chemical is crucial. In biochemical production, pyruvate and acetyl-CoA are primary building blocks. When sugar is used as the sole biosynthetic substrate, acetyl-CoA is commonly generated by pyruvate decarboxylation. However, pyruvate decarboxylation during acetyl-CoA formation limits the theoretical maximum carbon yield (TMCY) by releasing carbon, and in some cases also leads to redox imbalance. To avoid these problems, we describe here the construction of a metabolic pathway that simultaneously utilizes glucose and acetate. Acetate is utilized to produce acetyl-CoA without carbon loss or redox imbalance. We demonstrate the utility of this approach for isobutyl acetate (IBA) production, wherein IBA production with glucose and acetate achieves a higher carbon yield than with either sole carbon source. These results highlight the potential for this multiple carbon source approach to improve the TMCY and balance redox in biosynthetic pathways. PMID:26108471

47 CFR 76.980 - Charges for customer changes.

Code of Federal Regulations, 2012 CFR

2012-10-01

....980 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) BROADCAST RADIO SERVICES... charge for customer changes in service tiers effected solely by coded entry on a computer terminal or by... involve more than coded entry on a computer or other similarly simple method shall be based on actual cost...

47 CFR 76.980 - Charges for customer changes.

Code of Federal Regulations, 2011 CFR

2011-10-01

....980 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) BROADCAST RADIO SERVICES... charge for customer changes in service tiers effected solely by coded entry on a computer terminal or by... involve more than coded entry on a computer or other similarly simple method shall be based on actual cost...

47 CFR 76.980 - Charges for customer changes.

Code of Federal Regulations, 2014 CFR

2014-10-01

....980 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) BROADCAST RADIO SERVICES... charge for customer changes in service tiers effected solely by coded entry on a computer terminal or by... involve more than coded entry on a computer or other similarly simple method shall be based on actual cost...

47 CFR 76.980 - Charges for customer changes.

Code of Federal Regulations, 2013 CFR

2013-10-01

....980 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) BROADCAST RADIO SERVICES... charge for customer changes in service tiers effected solely by coded entry on a computer terminal or by... involve more than coded entry on a computer or other similarly simple method shall be based on actual cost...

47 CFR 76.980 - Charges for customer changes.

Code of Federal Regulations, 2010 CFR

2010-10-01

....980 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) BROADCAST RADIO SERVICES... charge for customer changes in service tiers effected solely by coded entry on a computer terminal or by... involve more than coded entry on a computer or other similarly simple method shall be based on actual cost...

7 CFR 4290.310 - Contents of application.

Code of Federal Regulations, 2010 CFR

2010-01-01

... (a)(1) of that section, as determined solely by the Secretary; (3) Has a viable business plan... business plan. The Applicant must submit a comprehensive business plan covering at least a five-year period... Agriculture Regulations of the Department of Agriculture (Continued) RURAL BUSINESS-COOPERATIVE SERVICE AND...

49 CFR 1242.86 - Industrial development (account XX-61-90).

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 9 2010-10-01 2010-10-01 false Industrial development (account XX-61-90). 1242.86 Section 1242.86 Transportation Other Regulations Relating to Transportation (Continued) SURFACE....86 Industrial development (account XX-61-90). These accounts pertain solely to freight service and...

32 CFR 634.15 - Restricted driving privileges or probation.

Code of Federal Regulations, 2012 CFR

2012-07-01

... assigned duties. In this instance, a limited exception can be granted for the sole purpose of driving... 32 National Defense 4 2012-07-01 2011-07-01 true Restricted driving privileges or probation. 634... (CONTINUED) LAW ENFORCEMENT AND CRIMINAL INVESTIGATIONS MOTOR VEHICLE TRAFFIC SUPERVISION Driving Privileges...

32 CFR 634.15 - Restricted driving privileges or probation.

Code of Federal Regulations, 2014 CFR

2014-07-01

... assigned duties. In this instance, a limited exception can be granted for the sole purpose of driving... 32 National Defense 4 2014-07-01 2013-07-01 true Restricted driving privileges or probation. 634... (CONTINUED) LAW ENFORCEMENT AND CRIMINAL INVESTIGATIONS MOTOR VEHICLE TRAFFIC SUPERVISION Driving Privileges...

32 CFR 634.15 - Restricted driving privileges or probation.

Code of Federal Regulations, 2013 CFR

2013-07-01

... assigned duties. In this instance, a limited exception can be granted for the sole purpose of driving... 32 National Defense 4 2013-07-01 2013-07-01 false Restricted driving privileges or probation. 634... (CONTINUED) LAW ENFORCEMENT AND CRIMINAL INVESTIGATIONS MOTOR VEHICLE TRAFFIC SUPERVISION Driving Privileges...

32 CFR 634.15 - Restricted driving privileges or probation.

Code of Federal Regulations, 2011 CFR

2011-07-01

... assigned duties. In this instance, a limited exception can be granted for the sole purpose of driving... 32 National Defense 4 2011-07-01 2011-07-01 false Restricted driving privileges or probation. 634... (CONTINUED) LAW ENFORCEMENT AND CRIMINAL INVESTIGATIONS MOTOR VEHICLE TRAFFIC SUPERVISION Driving Privileges...

42 CFR 489.18 - Change of ownership or leasing: Effect on provider agreement.

Code of Federal Regulations, 2010 CFR

2010-10-01

... HEALTH AND HUMAN SERVICES (CONTINUED) STANDARDS AND CERTIFICATION PROVIDER AGREEMENTS AND SUPPLIER..., constitutes change of ownership. (2) Unincorporated sole proprietorship. Transfer of title and property to...) Conditions that apply to assigned agreements. An assigned agreement is subject to all applicable statutes and...

49 CFR 574.4 - Applicability.

Code of Federal Regulations, 2010 CFR

2010-10-01

..., DEPARTMENT OF TRANSPORTATION (CONTINUED) TIRE IDENTIFICATION AND RECORDKEEPING § 574.4 Applicability. This... retreaded tires, and new non-pneumatic tires and non-pneumatic tire assemblies for use on motor vehicles... does not apply to persons who retread tires solely for their own use. [36 FR 5422, Mar. 23, 1971, as...

31 CFR 409.1 - Standard.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 31 Money and Finance:Treasury 2 2012-07-01 2012-07-01 false Standard. 409.1 Section 409.1 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) SECRET SERVICE, DEPARTMENT... the Secret Service will be guided solely by the principle of whether the applicant presents a...

31 CFR 409.1 - Standard.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 31 Money and Finance: Treasury 2 2014-07-01 2014-07-01 false Standard. 409.1 Section 409.1 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) SECRET SERVICE, DEPARTMENT... the Secret Service will be guided solely by the principle of whether the applicant presents a...

31 CFR 409.1 - Standard.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 31 Money and Finance:Treasury 2 2011-07-01 2011-07-01 false Standard. 409.1 Section 409.1 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) SECRET SERVICE, DEPARTMENT... the Secret Service will be guided solely by the principle of whether the applicant presents a...

31 CFR 409.1 - Standard.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Standard. 409.1 Section 409.1 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) SECRET SERVICE, DEPARTMENT... the Secret Service will be guided solely by the principle of whether the applicant presents a...

31 CFR 409.1 - Standard.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 31 Money and Finance:Treasury 2 2013-07-01 2013-07-01 false Standard. 409.1 Section 409.1 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) SECRET SERVICE, DEPARTMENT... the Secret Service will be guided solely by the principle of whether the applicant presents a...

Learning with Calculators: Doing More with Less

ERIC Educational Resources Information Center

Kissane, Barry

2017-01-01

It seems that calculators continue to be misunderstood as devices solely for calculation, although the likely contributions to learning mathematics with modern calculators arise from other characteristics. A four-part model to understand the educational significance of calculators underpins this paper. Each of the four components (representation,…

Pushing the Envelope

NASA Image and Video Library

2017-10-12

The first generation X-1 aircraft changed aviation history in numerous ways, and not simply because they were the first aircraft to fly faster than the speed of sound. Rather, they established the concept of the research aircraft, built solely for experimental purposes. NASA continues this legacy of experimental aircraft today.

21 CFR 558.95 - Bambermycins.

Code of Federal Regulations, 2014 CFR

2014-04-01

... week after date of manufacture. (c) [Reserved] (d) Conditions of use—(1) Chickens. Use in medicated... chickens: For increased rate of weight gain and improved feed efficiency Feed continuously as the sole... International Feed No. Percent Deflorinated phosphate (20.5% calcium, 18.5% phosphorus) 6-01-080 42.50 Sodium...

42 CFR 414.313 - Initial method of payment.

Code of Federal Regulations, 2010 CFR

2010-10-01

... inpatients who were not admitted solely to receive maintenance dialysis. (iv) Administration of hepatitis B... (CONTINUED) MEDICARE PROGRAM PAYMENT FOR PART B MEDICAL AND OTHER HEALTH SERVICES Determination of Reasonable... of this subchapter. (b) Services for which payment is not included in the add-on payment. (1...

12 CFR 263.405 - Petition for reinstatement.

Code of Federal Regulations, 2012 CFR

2012-01-01

... SYSTEM (CONTINUED) RULES OF PRACTICE FOR HEARINGS Removal, Suspension, and Debarment of Accountants From... ordered by the Board, a petition for reinstatement by an independent public accountant, an accounting firm... under this section may, in the sole discretion of the Board, be afforded a hearing. The accountant or...

12 CFR 263.405 - Petition for reinstatement.

Code of Federal Regulations, 2014 CFR

2014-01-01

... SYSTEM (CONTINUED) RULES OF PRACTICE FOR HEARINGS Removal, Suspension, and Debarment of Accountants From... ordered by the Board, a petition for reinstatement by an independent public accountant, an accounting firm... under this section may, in the sole discretion of the Board, be afforded a hearing. The accountant or...

12 CFR 263.405 - Petition for reinstatement.

Code of Federal Regulations, 2013 CFR

2013-01-01

... SYSTEM (CONTINUED) RULES OF PRACTICE FOR HEARINGS Removal, Suspension, and Debarment of Accountants From... ordered by the Board, a petition for reinstatement by an independent public accountant, an accounting firm... under this section may, in the sole discretion of the Board, be afforded a hearing. The accountant or...

Improving Quality of Shoe Soles Product using Six Sigma

NASA Astrophysics Data System (ADS)

Jesslyn Wijaya, Athalia; Trusaji, Wildan; Akbar, Muhammad; Ma’ruf, Anas; Irianto, Dradjad

2018-03-01

A manufacture in Bandung produce kind of rubber-based product i.e. trim, rice rollers, shoe soles, etc. After penetrating the shoe soles market, the manufacture has met customer with tight quality control. Based on the past data, defect level of this product was 18.08% that caused the manufacture’s loss of time and money. Quality improvement effort was done using six sigma method that included phases of define, measure, analyse, improve, and control (DMAIC). In the design phase, the object’s problem and definition were defined. Delphi method was also used in this phase to identify critical factors. In the measure phase, the existing process stability and sigma quality level were measured. Fishbone diagram and failure mode and effect analysis (FMEA) were used in the next phase to analyse the root cause and determine the priority issues. Improve phase was done by designing alternative improvement strategy using 5W1H method. Some improvement efforts were identified, i.e. (i) modifying design of the hanging rack, (ii) create pantone colour book and check sheet, (iii) provide pedestrian line at compound department, (iv) buying stop watch, and (v) modifying shoe soles dies. Some control strategies for continuous improvement were proposed such as SOP or reward and punishment system.

Acral peeling skin syndrome in two East-African siblings: case report

PubMed Central

2012-01-01

Background Acral peeling skin syndrome is a rare autosomal recessive genodermatosis due to a missense mutation in transglutaminase 5. The skin peeling occurs at the separation of the stratum corneum from the stratum granulosum. Case presentation We present a case of two siblings who developed continuous peeling of the palms and soles from the first year of life. This peeling was more severe on the soles than palms and on younger sibling than elder sibling. Peeling is worsened by occlusion and sweating. Conclusions Sporadic cases of Acral Peeling Skin Syndrome occur in African population. There is variability in time of presentation and clinical severity even within families. PMID:22429841

Isolation and Characterization of Rhamnolipid-Producing Bacterial Strains from a Biodiesel Facility

USDA-ARS?s Scientific Manuscript database

Novel strains of rhamnolipid-producing bacteria were isolated from soils at a biodiesel facility on the basis of their ability to grow on glycerol as a sole carbon source. Strains were identified as Acinetobacter calcoaceticus, Enterobacter asburiae, E. hormaecheii, Pantoea stewartii and Pseudomona...

48 CFR 8.405-6 - Limiting sources.

Code of Federal Regulations, 2013 CFR

2013-10-01

... or BPA with an estimated value exceeding the micro-purchase threshold not placed or established in... Schedule ordering procedures. The original order or BPA must not have been previously issued under sole... order or BPA exceeding the simplified acquisition threshold. (2) Posting. (i) Within 14 days after...

48 CFR 8.405-6 - Limiting sources.

Code of Federal Regulations, 2011 CFR

2011-10-01

... or BPA with an estimated value exceeding the micro-purchase threshold not placed or established in... Schedule ordering procedures. The original order or BPA must not have been previously issued under sole... order or BPA exceeding the simplified acquisition threshold. (2) Posting. (i) Within 14 days after...

48 CFR 8.405-6 - Limiting sources.

Code of Federal Regulations, 2012 CFR

2012-10-01

... or BPA with an estimated value exceeding the micro-purchase threshold not placed or established in... Schedule ordering procedures. The original order or BPA must not have been previously issued under sole... order or BPA exceeding the simplified acquisition threshold. (2) Posting. (i) Within 14 days after...

48 CFR 8.405-6 - Limiting sources.

Code of Federal Regulations, 2014 CFR

2014-10-01

... or BPA with an estimated value exceeding the micro-purchase threshold not placed or established in... Schedule ordering procedures. The original order or BPA must not have been previously issued under sole... order or BPA exceeding the simplified acquisition threshold. (2) Posting. (i) Within 14 days after...

Impact of cascadia subduction zone earthquake on the seismic evaluation criteria of bridges : technical report : SPR 770.

DOT National Transportation Integrated Search

2016-12-01

A large magnitude long duration subduction earthquake is impending in the Pacific Northwest, which lies near the : Cascadia Subduction Zone (CSZ). Great subduction zone earthquakes are the largest earthquakes in the world and are the sole source : zo...

46 CFR 183.354 - Battery installations.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Battery installations. 183.354 Section 183.354 Shipping...) ELECTRICAL INSTALLATION Power Sources and Distribution Systems § 183.354 Battery installations. (a) Large batteries. Each large battery installation must be located in a locker, room or enclosed box solely...

Jose Vasconcelos, Philosopher-Librarian.

ERIC Educational Resources Information Center

Christensen, Paul Martin

Jose Vasconcelos (1882-1959), a major figure of modern Mexican education, also was instrumental in the development of the field of librarianship in Mexico. This research paper presents Vasconcelos' philosophy of librarianship through a detailed study of his writings and a review of major secondary sources. Relying solely on the subjective…

Psychological Testing and Psychological Assessment: A Review of Evidence and Issues.

ERIC Educational Resources Information Center

Meyer, Gregory J.; Finn, Stephen E.; Eyde, Lorraine D.; Kay, Gary G.; Moreland, Kevin L.; Dies, Robert R.; Eisman, Elena J.; Kubiszyn, Tom W.; Reed, Geoffrey M.

2001-01-01

Summarizes issues associated with psychological assessment, concluding that: psychological test validity is strong and is comparable to medical test validity; distinct assessment methods provide unique sources of information; and clinicians who rely solely on interviews are prone to incomplete understandings. Suggests that multimethod assessment…

The Image of Africa in Our Classrooms.

ERIC Educational Resources Information Center

Pahl, Ronald H.

1995-01-01

Argues that the media portrayal of Africa remains highly selective, focusing almost solely on negative and violent stories. Positive stories (local initiatives, political reform) go unreported as does much of the Western powers involvement in famine and destruction. Includes a list of sources on African current affairs. (MJP)

46 CFR 183.354 - Battery installations.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 7 2012-10-01 2012-10-01 false Battery installations. 183.354 Section 183.354 Shipping...) ELECTRICAL INSTALLATION Power Sources and Distribution Systems § 183.354 Battery installations. (a) Large batteries. Each large battery installation must be located in a locker, room or enclosed box solely...

46 CFR 183.354 - Battery installations.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 7 2013-10-01 2013-10-01 false Battery installations. 183.354 Section 183.354 Shipping...) ELECTRICAL INSTALLATION Power Sources and Distribution Systems § 183.354 Battery installations. (a) Large batteries. Each large battery installation must be located in a locker, room or enclosed box solely...

46 CFR 183.354 - Battery installations.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 7 2011-10-01 2011-10-01 false Battery installations. 183.354 Section 183.354 Shipping...) ELECTRICAL INSTALLATION Power Sources and Distribution Systems § 183.354 Battery installations. (a) Large batteries. Each large battery installation must be located in a locker, room or enclosed box solely...

46 CFR 183.354 - Battery installations.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 7 2014-10-01 2014-10-01 false Battery installations. 183.354 Section 183.354 Shipping...) ELECTRICAL INSTALLATION Power Sources and Distribution Systems § 183.354 Battery installations. (a) Large batteries. Each large battery installation must be located in a locker, room or enclosed box solely...

48 CFR 2901.603-70 - Responsibility of other government personnel.

Code of Federal Regulations, 2010 CFR

2010-10-01

... buying process, and execution of the contract rests with the contracting officer. (b) Personnel... obligation. However, DOL personnel who must use the contracting process to accomplish their programs must... and open competition are satisfied to the maximum extent practicable. Sole source purchases may only...

A fungal transcription factor essential for starch degradation affects integration of carbon and nitrogen metabolism

DOE PAGES

Xiong, Yi; Wu, Vincent W.; Lubbe, Andrea; ...

2017-05-03

In Neurospora crassa, the transcription factor COL-26 functions as a regulator of glucose signaling and metabolism. Its loss leads to resistance to carbon catabolite repression. Here, we report that COL-26 is necessary for the expression of amylolytic genes in N. crassa and is required for the utilization of maltose and starch. Additionally, the Δcol-26 mutant shows growth defects on preferred carbon sources, such as glucose, an effect that was alleviated if glutamine replaced ammonium as the primary nitrogen source. This rescue did not occur when maltose was used as a sole carbon source. Transcriptome and metabolic analyses of the Δcol-26more » mutant relative to its wild type parental strain revealed that amino acid and nitrogen metabolism, the TCA cycle and GABA shunt were adversely affected. Phylogenetic analysis showed a single col-26 homolog in Sordariales, Ophilostomatales, and the Magnaporthales, but an expanded number of col-26 homologs in other filamentous fungal species. Deletion of the closest homolog of col-26 in Trichoderma reesei, bglR, resulted in a mutant with similar preferred carbon source growth deficiency, and which was alleviated if glutamine was the sole nitrogen source, suggesting conservation of COL-26 and BglR function. Our finding provides novel insight into the role of COL-26 for utilization of starch and in integrating carbon and nitrogen metabolism for balanced metabolic activities for optimal carbon and nitrogen distribution.« less

A fungal transcription factor essential for starch degradation affects integration of carbon and nitrogen metabolism

DOE Office of Scientific and Technical Information (OSTI.GOV)

Xiong, Yi; Wu, Vincent W.; Lubbe, Andrea

In Neurospora crassa, the transcription factor COL-26 functions as a regulator of glucose signaling and metabolism. Its loss leads to resistance to carbon catabolite repression. Here, we report that COL-26 is necessary for the expression of amylolytic genes in N. crassa and is required for the utilization of maltose and starch. Additionally, the Δcol-26 mutant shows growth defects on preferred carbon sources, such as glucose, an effect that was alleviated if glutamine replaced ammonium as the primary nitrogen source. This rescue did not occur when maltose was used as a sole carbon source. Transcriptome and metabolic analyses of the Δcol-26more » mutant relative to its wild type parental strain revealed that amino acid and nitrogen metabolism, the TCA cycle and GABA shunt were adversely affected. Phylogenetic analysis showed a single col-26 homolog in Sordariales, Ophilostomatales, and the Magnaporthales, but an expanded number of col-26 homologs in other filamentous fungal species. Deletion of the closest homolog of col-26 in Trichoderma reesei, bglR, resulted in a mutant with similar preferred carbon source growth deficiency, and which was alleviated if glutamine was the sole nitrogen source, suggesting conservation of COL-26 and BglR function. Our finding provides novel insight into the role of COL-26 for utilization of starch and in integrating carbon and nitrogen metabolism for balanced metabolic activities for optimal carbon and nitrogen distribution.« less

Independent Component Analysis-motivated Approach to Classificatory Decomposition of Cortical Evoked Potentials

PubMed Central

Smolinski, Tomasz G; Buchanan, Roger; Boratyn, Grzegorz M; Milanova, Mariofanna; Prinz, Astrid A

2006-01-01

Background Independent Component Analysis (ICA) proves to be useful in the analysis of neural activity, as it allows for identification of distinct sources of activity. Applied to measurements registered in a controlled setting and under exposure to an external stimulus, it can facilitate analysis of the impact of the stimulus on those sources. The link between the stimulus and a given source can be verified by a classifier that is able to "predict" the condition a given signal was registered under, solely based on the components. However, the ICA's assumption about statistical independence of sources is often unrealistic and turns out to be insufficient to build an accurate classifier. Therefore, we propose to utilize a novel method, based on hybridization of ICA, multi-objective evolutionary algorithms (MOEA), and rough sets (RS), that attempts to improve the effectiveness of signal decomposition techniques by providing them with "classification-awareness." Results The preliminary results described here are very promising and further investigation of other MOEAs and/or RS-based classification accuracy measures should be pursued. Even a quick visual analysis of those results can provide an interesting insight into the problem of neural activity analysis. Conclusion We present a methodology of classificatory decomposition of signals. One of the main advantages of our approach is the fact that rather than solely relying on often unrealistic assumptions about statistical independence of sources, components are generated in the light of a underlying classification problem itself. PMID:17118151

A fungal transcription factor essential for starch degradation affects integration of carbon and nitrogen metabolism

PubMed Central

Xiong, Yi; Qin, Lina; Kennedy, Megan; Bauer, Diane; Barry, Kerrie; Northen, Trent R.; Grigoriev, Igor V.

2017-01-01

In Neurospora crassa, the transcription factor COL-26 functions as a regulator of glucose signaling and metabolism. Its loss leads to resistance to carbon catabolite repression. Here, we report that COL-26 is necessary for the expression of amylolytic genes in N. crassa and is required for the utilization of maltose and starch. Additionally, the Δcol-26 mutant shows growth defects on preferred carbon sources, such as glucose, an effect that was alleviated if glutamine replaced ammonium as the primary nitrogen source. This rescue did not occur when maltose was used as a sole carbon source. Transcriptome and metabolic analyses of the Δcol-26 mutant relative to its wild type parental strain revealed that amino acid and nitrogen metabolism, the TCA cycle and GABA shunt were adversely affected. Phylogenetic analysis showed a single col-26 homolog in Sordariales, Ophilostomatales, and the Magnaporthales, but an expanded number of col-26 homologs in other filamentous fungal species. Deletion of the closest homolog of col-26 in Trichoderma reesei, bglR, resulted in a mutant with similar preferred carbon source growth deficiency, and which was alleviated if glutamine was the sole nitrogen source, suggesting conservation of COL-26 and BglR function. Our finding provides novel insight into the role of COL-26 for utilization of starch and in integrating carbon and nitrogen metabolism for balanced metabolic activities for optimal carbon and nitrogen distribution. PMID:28467421

Characterisation of a resolution enhancing image inversion interferometer.

PubMed

Wicker, Kai; Sindbert, Simon; Heintzmann, Rainer

2009-08-31

Image inversion interferometers have the potential to significantly enhance the lateral resolution and light efficiency of scanning fluorescence microscopes. Self-interference of a point source's coherent point spread function with its inverted copy leads to a reduction in the integrated signal for off-axis sources compared to sources on the inversion axis. This can be used to enhance the resolution in a confocal laser scanning microscope. We present a simple image inversion interferometer relying solely on reflections off planar surfaces. Measurements of the detection point spread function for several types of light sources confirm the predicted performance and suggest its usability for scanning confocal fluorescence microscopy.

45 CFR 1703.302 - Role of observers.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 45 Public Welfare 4 2011-10-01 2011-10-01 false Role of observers. 1703.302 Section 1703.302 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL COMMISSION ON LIBRARIES AND... public may attend open meetings for the sole purpose of observation and may not record any of the...

45 CFR 1703.302 - Role of observers.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 45 Public Welfare 4 2010-10-01 2010-10-01 false Role of observers. 1703.302 Section 1703.302 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL COMMISSION ON LIBRARIES AND... public may attend open meetings for the sole purpose of observation and may not record any of the...

40 CFR 168.66 - Labeling of pesticide products and devices intended solely for export.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 25 2013-07-01 2013-07-01 false Labeling of pesticide products and... PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS STATEMENTS OF ENFORCEMENT POLICIES AND INTERPRETATIONS Export Policy and Procedures for Exporting Pesticides § 168.66 Labeling of pesticide products and devices...

40 CFR 168.66 - Labeling of pesticide products and devices intended solely for export.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 24 2014-07-01 2014-07-01 false Labeling of pesticide products and... PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS STATEMENTS OF ENFORCEMENT POLICIES AND INTERPRETATIONS Export Policy and Procedures for Exporting Pesticides § 168.66 Labeling of pesticide products and devices...

BY HOW MUCH DO SHAPES OF TOXICOLOGICAL DOSE-RESPONSE RELATIONSHIPS VARY? (SOT)

EPA Science Inventory

A re-analysis of a large number of historical dose-response data for continuous endpoints showed that the shapes of the dose-response relationships were surprisingly homogenous. The datasets were selected on the sole criterion that they were expected to provide relatively good in...

Age Specific Programming.

ERIC Educational Resources Information Center

Morse, Neil

Are children going to continue to serve television by providing a trusting audience for its commercial message, or is television going to begin to serve children? Current children's programs are designed for the sole purpose of holding the attention of the broadest age span possible. Today's television fails to enhance the small child's…

Commentary: You're Not Studying, You're Just...

ERIC Educational Resources Information Center

Purushotma, Ravi

2005-01-01

As often as language teachers lecture about the importance of continual practice to adolescent learners, the dullness of homework exercises designed primarily to be educational has difficulty competing with popular media designed solely to be entertaining. Recently, numerous attempts have been made to develop "edutainment" titles that seek to…

Seeking Excellence in Counselor Education: One School's Journey

ERIC Educational Resources Information Center

Sheehan, Timothy J.

2003-01-01

Assuring learning outcomes that result in expert alcohol and drug dependency counseling is a continuous challenge for educators. Working systemically to craft a solid vision for the future, the Hazelden Foundation developed a graduate school for the sole purpose of educating highly skilled clinicians. Key ingredients included building a…

19 CFR 146.44 - Zone-restricted status.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 19 Customs Duties 2 2010-04-01 2010-04-01 false Zone-restricted status. 146.44 Section 146.44... TREASURY (CONTINUED) FOREIGN TRADE ZONES Status of Merchandise in a Zone § 146.44 Zone-restricted status. (a) General. Merchandise taken into a zone for the sole purpose of exportation, destruction (except...

29 CFR 1918.106 - Payment for protective equipment.

Code of Federal Regulations, 2011 CFR

2011-07-01

...) Ordinary clothing, skin creams, or other items, used solely for protection from weather, such as winter... 29 Labor 7 2011-07-01 2011-07-01 false Payment for protective equipment. 1918.106 Section 1918.106..., DEPARTMENT OF LABOR (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Personal Protective Equipment...

29 CFR 1926.95 - Criteria for personal protective equipment.

Code of Federal Regulations, 2010 CFR

2010-07-01

... shoes, and normal work boots; or (ii) Ordinary clothing, skin creams, or other items, used solely for... 29 Labor 8 2010-07-01 2010-07-01 false Criteria for personal protective equipment. 1926.95 Section..., DEPARTMENT OF LABOR (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR CONSTRUCTION Personal Protective and Life...

29 CFR 1926.95 - Criteria for personal protective equipment.

Code of Federal Regulations, 2011 CFR

2011-07-01

... shoes, and normal work boots; or (ii) Ordinary clothing, skin creams, or other items, used solely for... 29 Labor 8 2011-07-01 2011-07-01 false Criteria for personal protective equipment. 1926.95 Section..., DEPARTMENT OF LABOR (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR CONSTRUCTION Personal Protective and Life...

29 CFR 1918.106 - Payment for protective equipment.

Code of Federal Regulations, 2010 CFR

2010-07-01

...) Ordinary clothing, skin creams, or other items, used solely for protection from weather, such as winter... 29 Labor 7 2010-07-01 2010-07-01 false Payment for protective equipment. 1918.106 Section 1918.106..., DEPARTMENT OF LABOR (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Personal Protective Equipment...

The Neural Basis of Speech Parsing in Children and Adults

ERIC Educational Resources Information Center

McNealy, Kristin; Mazziotta, John C.; Dapretto, Mirella

2010-01-01

Word segmentation, detecting word boundaries in continuous speech, is a fundamental aspect of language learning that can occur solely by the computation of statistical and speech cues. Fifty-four children underwent functional magnetic resonance imaging (fMRI) while listening to three streams of concatenated syllables that contained either high…

46 CFR 42.05-45 - International voyage.

Code of Federal Regulations, 2014 CFR

2014-10-01

... COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) LOAD LINES DOMESTIC AND FOREIGN VOYAGES BY SEA...) of the 1966 Convention, an international voyage means a sea voyage from any country to a port outside... solely navigating the Great Lakes. Accordingly, such vessels shall not be considered as being on an...

46 CFR 42.05-45 - International voyage.

Code of Federal Regulations, 2011 CFR

2011-10-01

... COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) LOAD LINES DOMESTIC AND FOREIGN VOYAGES BY SEA...) of the 1966 Convention, an international voyage means a sea voyage from any country to a port outside... solely navigating the Great Lakes. Accordingly, such vessels shall not be considered as being on an...

46 CFR 42.05-45 - International voyage.

Code of Federal Regulations, 2012 CFR

2012-10-01

... COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) LOAD LINES DOMESTIC AND FOREIGN VOYAGES BY SEA...) of the 1966 Convention, an international voyage means a sea voyage from any country to a port outside... solely navigating the Great Lakes. Accordingly, such vessels shall not be considered as being on an...

46 CFR 42.05-45 - International voyage.

Code of Federal Regulations, 2013 CFR

2013-10-01

... COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) LOAD LINES DOMESTIC AND FOREIGN VOYAGES BY SEA...) of the 1966 Convention, an international voyage means a sea voyage from any country to a port outside... solely navigating the Great Lakes. Accordingly, such vessels shall not be considered as being on an...

21 CFR 558.680 - Zoalene.

Code of Federal Regulations, 2014 CFR

2014-04-01

... by No. 054771 in § 510.600(c) of this chapter 054771 (2) Turkeys— Zoalene in grams/ton Combination in grams per ton Indications for use Limitations Sponsor (i) 113.5 to 170.3 Growing turkeys: For prevention and control of coccidiosis Feed continuously as sole ration. For turkeys grown for meat purposes only...

Engineering the Space Age: A Rocket Scientist Remembers

DTIC Science & Technology

2008-07-01

energy sources -Research I. Title. 629.130092-dc22 Disclaimer Opinions, conclusions, and recommendations expressed or implied within are solely those of the...technical armed forces oc- 12 AERONAUTICAL ENGINEERING cupation specialty and keep flying. At the time, the atomic bombs were bulky and heavy and could only...way to learn about the F-84 aircraft. A welcome respite occurred when Major Wailer sent me to RAC to monitor a test to find the source and correct the

Evaluation of a Recombinant Escherichia coli Strain that Uses the Sarin Simulant Isopropylmethylphosphonic Acid (IMPA) as a Sole Carbon and Phosphate Source

DTIC Science & Technology

2016-04-01

phosphate use by these recombinant strains was evaluated because carbon use by these strains is still undergoing optimization by LBNL. The E . coli ...plasmids, had successful growth when transformed into a different E . coli background, which correlated with IMPA degradation. Ultimately, the...transformed E . coli strains, optimized at ECBC, were able to grow using IMPA as the phosphate source. 15. SUBJECT TERMS Acetylcholinesterase (AChE

Isolation and Characterization of Anaerobic Bacteria for Symbiotic Recycling of Uric Acid Nitrogen in the Gut of Various Termites

PubMed Central

Thong-On, Arunee; Suzuki, Katsuyuki; Noda, Satoko; Inoue, Jun-ichi; Kajiwara, Susumu; Ohkuma, Moriya

2012-01-01

Recycling of the nitrogenous waste uric acid (UA) of wood-feeding termites by their gut bacteria is one of the significant aspects of symbiosis for the conservation of nitrogen sources. Diverse anaerobic UA-degrading bacteria comprising 16 species were isolated from the gut of eight termite species, and were assigned to Clostridia, Enterobacteriaceae, and low G+C Gram-positive cocci. UA-degrading Clostridia had never been isolated from termite guts. UA-degrading ability was sporadically distributed among phylogenetically various culturable anaerobic bacteria from termite guts. A strain of Clostridium sp., which was commonly isolated from three termite species and represented a probable new species in cluster XIVa of clostridia, utilized UA as a nitrogen source but not as a sole carbon and energy source. This feature is in clear contrast to that of well-studied purinolytic clostridia or previously isolated UA degraders from termite guts, which also utilize UA as a sole carbon and energy source. Ammonia is the major nitrogenous product of UA degradation. Various purines stimulated the growth of this strain when added to an otherwise growth-limiting, nitrogen poor medium. The bacterial species involved the recycling of UA nitrogen in the gut microbial community of termites are more diverse in terms of both taxonomy and nutritional physiology than previously recognized. PMID:22791052

Microbial removal of alkanes from dilute gaseous waste streams: kinetics and mass transfer considerations.

PubMed

Barton, J W; Klasson, K T; Koran, L J; Davison, B H

1997-01-01

Treatment of dilute gaseous hydrocarbon waste streams remains a current need for many industries, particularly as increasingly stringent environmental regulations and oversight force emission reduction. Biofiltration systems hold promise for providing low-cost alternatives to more traditional, energy-intensive treatment methods such as incineration and adsorption. Elucidation of engineering principles governing the behavior of such systems, including mass transfer limitations, will broaden their applicability. Our processes exploit a microbial consortium to treat a mixture of 0.5% n-pentane and 0.5% isobutane in air. Since hydrocarbon gases are sparingly soluble in water, good mixing and high surface area between the gas and liquid phases are essential for biodegradation to be effective. One liquid-continuous columnar bioreactor was operated for more than 30 months with continued degradation of n-pentane and isobutane as sole carbon and energy sources. The maximum degradation rate observed in this gas-recycle system was 2 g of volatile organic compounds (VOC)/(m3.h). A trickle-bed bioreactor was operated continuously for over 24 months to provide a higher surface area (using a structured packing) with increased rates. Degradation rates consistently achieved were approximately 50 g of VOC/(m3.h) via single pass in this gas-continuous columnar system. Effective mass transfer coefficients comparable to literature values were also measured for this reactor; these values were substantially higher than those found in the gas-recycle reactor. Control of biomass levels was implemented by limiting the level of available nitrogen in the recirculating aqueous media, enabling long-term stability of reactor performance.

21 CFR 520.2640 - Tylosin.

Code of Federal Regulations, 2010 CFR

2010-04-01

... use—(1) Chickens—(i) Amount. 2 grams per gallon for 1 to 5 days as the sole source of drinking water. Treated chickens should consume enough medicated drinking water to provide 50 milligrams (mg) tylosin per... sensitive to tylosin at time of vaccination or other stress in chickens. For the control of chronic...

21 CFR 520.2640 - Tylosin.

Code of Federal Regulations, 2011 CFR

2011-04-01

...) Amount. 2 grams per gallon for 1 to 5 days as the sole source of drinking water. Treated chickens should consume enough medicated drinking water to provide 50 milligrams (mg) tylosin per pound of body weight per... of vaccination or other stress in chickens. For the control of chronic respiratory disease (CRD...

AACRAO's Basic Guide to Enrollment Management

ERIC Educational Resources Information Center

Westman, Craig

2005-01-01

The sole purpose of this book is to provide a primer or "how-to" resource for new or seasoned enrollment managers. Its core chapters are practical applications of Strategic Enrollment Management (SEM) best practices. While incorporating some secondary source referencing, the dialogue primarily outlines how making some basic adjustments to business…

48 CFR 217.7505 - Limitations on price increases.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Limitations on price... Acquisition of Replenishment Parts 217.7505 Limitations on price increases. This section provides implementing... award, on a sole source basis, a contract for any centrally managed replenishment part when the price of...

Effects of artemisinin and Artemisia annua extracts on Haemonchus contortus in gerbils (Meriones unguiculatus)

USDA-ARS?s Scientific Manuscript database

Haemonchus contortus is a blood-sucking abomasal parasite of small ruminants that is responsible for major losses to producers worldwide. Resistance of this nematode to commercial anthelmintics has produced a demand for alternative control methods. Artemisia annua is the sole commercial source of ...

48 CFR 619.811-1 - Sole source.

Code of Federal Regulations, 2011 CFR

2011-10-01

... contracting activity shall include SBA's requirement number on the award document. (4) A single award document... sign the contract as a third party. The 8(a) contractor's signature shall be placed on the award... and open competition. (2) The contracting officer shall insert FAR 52.219-14, Limitations on...

48 CFR 619.811-1 - Sole source.

Code of Federal Regulations, 2010 CFR

2010-10-01

... contracting activity shall include SBA's requirement number on the award document. (4) A single award document... sign the contract as a third party. The 8(a) contractor's signature shall be placed on the award... and open competition. (2) The contracting officer shall insert FAR 52.219-14, Limitations on...

76 FR 55947 - Industrial Relations Promotion Project, Phase II in Vietnam

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-09

... to award sole source (Cooperative Agreement). SUMMARY: The U.S. Department of Labor (USDOL), Bureau.../Nathan Group LLC (DAI) for the purpose of implementing a program to strengthen compliance with... to perform the type of activity to be funded.. DAI, through its Industrial Relations Promotion...

46 CFR 129.356 - Battery installations.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Battery installations. 129.356 Section 129.356 Shipping... INSTALLATIONS Power Sources and Distribution Systems § 129.356 Battery installations. (a) Large. Each large battery-installation must be located in a locker, room, or enclosed box dedicated solely to the storage of...

Cellobiose fermenting yeast produces varied forms of native ß-glucosidase

USDA-ARS?s Scientific Manuscript database

The rapid growing yeast strain NRRL Y-50464 is robust to environmental stress and resistant to 2-furaldehyde (furfural) and 5-[hydroxymethyl]-2-furaldehyde (HMF). It is able to utilize cellobiose as its sole source of carbon and produces ethanol from lignocellulosic biomass by simultaneous saccharif...

OXIDATION OF BIPHENYL BY A MULTICOMPONENT ENZYME SYSTEM FROM PSEUDOMONAS SP. STRAIN LB400

EPA Science Inventory

Pseudomonas sp. strain LB400 grows on biphenyl as the sole carbon and energy source. This organism also cooxidizes several chlorinated biphenyl congeners. Biphenyl dioxygenase activity in cell extract required addition of NAD(P)H as an electron donor for the conversion of bipheny...

Applications of Peer Assessment and Self-Assessment in Music

ERIC Educational Resources Information Center

Valle, Christopher; Andrade, Heidi; Palma, Maria; Hefferen, Joanna

2016-01-01

Feedback is crucial to students' growth as musicians. Fortunately, the teacher is not the sole source of feedback in the music classroom. Under the right conditions, students can provide actionable feedback to themselves and each other. This article showcases the work of three elementary music specialists who have innovatively incorporated…

NOVEL ORGANIZATION OF THE GENES FOR PHTHALATE DEGRADATION FROM BURKHOLDERIA CEPACIA DBO1

EPA Science Inventory

Burkholderia cepacia DBO1 is able to utilize phthalate as the sole source of carbon and energy for growth. Two overlapping cosmid clones containing the genes for phthalate degradation were isolated from this strain. Subcloning and activity analysis localized the genes for phthala...

34 CFR 675.27 - Nature and source of institutional share.

Code of Federal Regulations, 2010 CFR

2010-07-01

... employment agreement to do so. (Approved by the Office of Management and Budget under control number 1840..., board, and books. (2) The institution shall document all amounts claimed as non-cash contributions. (3) Non-cash compensation may not include forgiveness of a charge assessed solely because of a student's...

BIODEGRADABILITY OF ALKYLATES AS A SOLE CARBON SOURCE IN THE PRESENCE OF ETHANOL OR BTEX

EPA Science Inventory

The biodegradability of alkylate compounds in serum bottles was investigated in the presence and absence of ethanol or benzene, toluene, ethylbenzene, and p-xylene (BTEX). The biomass was acclimated to three different alkylates, 2,3-dimethylpentane, 2,4-dimethylpentane, an...

75 FR 31288 - Plant-Verified Drop Shipment (PVDS)-Nonpostal Documentation

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-03

... POSTAL SERVICE 39 CFR Part 111 Plant-Verified Drop Shipment (PVDS)--Nonpostal Documentation AGENCY... 8125, Plant-Verified Drop Shipment (PVDS) Verification and Clearance, is the sole source of evidence... induction points of plant-verified drop shipment mailings, the Postal Service is adopting this final rule to...

Conversion of xylose to ethanol under aerobic conditions by Candida tropicalis

Treesearch

T. W. Jeffries

1981-01-01

Candida tropicalis converts xylose to ethanol under aerobic, but not anaerobic, conditions. Ethanol production lags behind growth and is accelerated by increased aeration. Adding xylose to active cultures stimulates ethanol production as does serial subculture in a medium containing xylose as a sole carbon source.

46 CFR 129.356 - Battery installations.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Battery installations. 129.356 Section 129.356 Shipping... INSTALLATIONS Power Sources and Distribution Systems § 129.356 Battery installations. (a) Large. Each large battery-installation must be located in a locker, room, or enclosed box dedicated solely to the storage of...

46 CFR 129.356 - Battery installations.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Battery installations. 129.356 Section 129.356 Shipping... INSTALLATIONS Power Sources and Distribution Systems § 129.356 Battery installations. (a) Large. Each large battery-installation must be located in a locker, room, or enclosed box dedicated solely to the storage of...

46 CFR 129.356 - Battery installations.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Battery installations. 129.356 Section 129.356 Shipping... INSTALLATIONS Power Sources and Distribution Systems § 129.356 Battery installations. (a) Large. Each large battery-installation must be located in a locker, room, or enclosed box dedicated solely to the storage of...

46 CFR 129.356 - Battery installations.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Battery installations. 129.356 Section 129.356 Shipping... INSTALLATIONS Power Sources and Distribution Systems § 129.356 Battery installations. (a) Large. Each large battery-installation must be located in a locker, room, or enclosed box dedicated solely to the storage of...

CLASSIFICATION OF PAH-DEGRADING BACTERIA BY PAH UTILIZATION PATTERNS AND THE COMPARISON OF METABOLIC PRODUCTS

EPA Science Inventory

Bacterial strains capable of using either phenanthrene, fluoranthene, or pyrene as sole carbon and energy sources were isolated from 16 different soil samples collected from the United States, Germany, and Norway. Thirty one strains were isolated on fluoranthene and the other twe...

76 FR 61362 - Proposed Data Collections Submitted for Public Comment and Recommendations

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-04

... deaths, marriages, and divorces. Similar data have been published since 1937 and are the sole source of... (county) officials in New Mexico who record marriages occurring and divorces and annulments granted in... occurrences of births, deaths, infant deaths, marriages, and divorces immediately following the month of...

Somatic proembryo production from excised, wounded zygotic carrot embryos on hormone-free medium: evaluation of the effects of pH, ethylene and activated charcoal

NASA Technical Reports Server (NTRS)

Smith, D. L.; Krikorian, A. D.

1990-01-01

Wounded zygotic embryos of cultivated carrot produce somatic proembryos on hormone-free nutrient medium containing 1 mM NH4+ as the sole nitrogen source. Continued maintenance of proembryos on this medium leads to a "pure" culture of preglobular stage proembryos (PGSPs). Ethylene had no effect on this process. Also, somatic embryo production was not affected by growing cultures on activated charcoal-impregnated filter papers. However, somatic proembyros initiated on activated charcoal papers were not maintainable as PGSPs and developed into later embryo stages. Normally, medium pH dropped from 5.7 to 4 during each subculture period, but when using activated charcoal papers the pH endpoint was around 6 - 7 due to a leachable substance(s) within the filter papers. When powdered, activated charcoal was used in the medium as an adsorbent of products potentially released after wounding, pH dropped at the normal rate and to the expected levels; proembryos did not mature into later embryo stages and were maintainable exclusively as PGSPs. Low pH (approximately 4) is detrimental to proembyro production, but is essential to maintaining PGSPs on hormone-free nutrient medium, whereas a sustained pH > or = 5.7 allows continued development of PGSPs into later embryo stages.

Single-cell protein from methanol with Enterobacter aerogenes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gnan, S.O.; Abodreheba, A.O.

1987-02-20

An identified Enterobacter aerogenes utilizing methanol as a sole carbon source was studied for the optimization of biomass production and the reduction of its nucleic acid content. Results indicated that the highest yield and conversion were obtained at 0.5% methanol. The addition of seawater as a source of trace elements has an adverse effect. However, the addition of urea as source of nitrogen enhanced the growth of E. aerogenes. Heat shock at 60 degrees C for one minute followed by incubation at 50 degrees C for 2 hours caused 72.6% reduction in the nucleic acid. 12 references.

Carbon metabolism influenced for promoters and temperature used in the heterologous protein production using Pichia pastoris yeast.

PubMed

Zepeda, Andrea B; Pessoa, Adalberto; Farías, Jorge G

2018-05-19

Nowadays, it is necessary to search for different high-scale production strategies to produce recombinant proteins of economic interest. Only a few microorganisms are industrially relevant for recombinant protein production: methylotrophic yeasts are known to use methanol efficiently as the sole carbon and energy source. Pichia pastoris is a methylotrophic yeast characterized as being an economical, fast and effective system for heterologous protein expression. Many factors can affect both the product and the production, including the promoter, carbon source, pH, production volume, temperature, and many others; but to control all of them most of the time is difficult and this depends on the initial selection of each variable. Therefore, this review focuses on the selection of the best promoter in the recombination process, considering different inductors, and the temperature as a culture medium variable in methylotrophic Pichia pastoris yeast. The goal is to understand the effects associated with different factors that influence its cell metabolism and to reach the construction of an expression system that fulfills the requirements of the yeast, presenting an optimal growth and development in batch, fed-batch or continuous cultures, and at the same time improve its yield in heterologous protein production. Copyright © 2018 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. All rights reserved.

Rand Corporation National Security Research Division. Annual Report 2010-2011

DTIC Science & Technology

2011-01-01

Approval of Sole-Source DoD Contracts to Native American Organizations A n nuA l R e p o R t 2 0 1 0 – 2 0 11 29 Alternative Fuels for the...average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed...shall be subject to a penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number. 1

Army Contracting Officials Could Have Purchased Husky Mounted Detection System Spare Parts at Lower Prices (Redacted)

DTIC Science & Technology

2016-03-31

system mounted on an armored vehicle . It detects and marks landmines and other buried explosive hazards, and serves an important role in keeping...countries. Figure 1. HMDS Shown on a Husky Vehicle Source: Project Manager Close Combat Systems FOR OFFICIAL USE ONLY FOR OFFICIAL USE ONLY Introduction...Reasonable Prices From Meggitt Aircraft Braking Systems for Sole-Source Commercial Spare Parts,” May 8, 2015 Report No. DODIG-2015-103, “Summary of

Exploring Fingerprints of the Extreme Thermoacidophile Metallosphaera sedula Grown on Synthetic Martian Regolith Materials as the Sole Energy Sources.

PubMed

Kölbl, Denise; Pignitter, Marc; Somoza, Veronika; Schimak, Mario P; Strbak, Oliver; Blazevic, Amir; Milojevic, Tetyana

2017-01-01

The biology of metal transforming microorganisms is of a fundamental and applied importance for our understanding of past and present biogeochemical processes on Earth and in the Universe. The extreme thermoacidophile Metallosphaera sedula is a metal mobilizing archaeon, which thrives in hot acid environments (optimal growth at 74°C and pH 2.0) and utilizes energy from the oxidation of reduced metal inorganic sources. These characteristics of M. sedula make it an ideal organism to further our knowledge of the biogeochemical processes of possible life on extraterrestrial planetary bodies. Exploring the viability and metal extraction capacity of M. sedula living on and interacting with synthetic extraterrestrial minerals, we show that M. sedula utilizes metals trapped in the Martian regolith simulants (JSC Mars 1A; P-MRS; S-MRS; MRS07/52) as the sole energy sources. The obtained set of microbiological and mineralogical data suggests that M. sedula actively colonizes synthetic Martian regolith materials and releases free soluble metals. The surface of bioprocessed Martian regolith simulants is analyzed for specific mineralogical fingerprints left upon M. sedula growth. The obtained results provide insights of biomining of extraterrestrial material as well as of the detection of biosignatures implementing in life search missions.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wear, Jr., John Edmund

The objective of this study was to examine the hypothesis that select functional groups of bacteria from pristine sites have an innate ability to degrade synthetic aromatics that often contaminate groundwater environments,due to exposure to naturally occurring recalcitrant aromatics in their environment. This study demonstrates that subsurface microbial communities are capable of utilizing lignin and humic acid breakdown products. Utilizers of these compounds were found to be present in most all the wells tested. Even the deepest aquifer tested had utilizers present for all six of the aromatics tested. Highest counts for the aromatics tested were observed with the naturallymore » occurring breakdown products of either lignin or humic acid. Carboxylic acids were found to be an important sole carbon source for groundwater bacteria possibly explained by the fact that they are produced by the oxidative cleavage of aromatic ring structures. The carbohydrate sole carbon sources that demonstrated the greatest densities were ones commonly associated with humics. This study indicates that utilization of naturally occurring aromatic compounds in the subsurface is an important nutritional source for groundwater bacteria. In addition, it suggests that adaptation to naturally occurring recalcitrant substrates is the origin of degradative pathways for xenobiotic compounds with analogous structure. This work has important implications for in situ bioremediation as a method of environmental cleanup.« less

Evolved hexose transporter enhances xylose uptake and glucose/xylose co-utilization in Saccharomyces cerevisiae

DOE Office of Scientific and Technical Information (OSTI.GOV)

Reider Apel, Amanda; Ouellet, Mario; Szmidt-Middleton, Heather

Enhancing xylose utilization has been a major focus in Saccharomyces cerevisiae strain-engineering efforts. The incentive for these studies arises from the need to use all sugars in the typical carbon mixtures that comprise standard renewable plant-biomass-based carbon sources. While major advances have been made in developing utilization pathways, the efficient import of five carbon sugars into the cell remains an important bottleneck in this endeavor. Here we use an engineered S. cerevisiae BY4742 strain, containing an established heterologous xylose utilization pathway, and imposed a laboratory evolution regime with xylose as the sole carbon source. We obtained several evolved strains withmore » improved growth phenotypes and evaluated the best candidate using genome resequencing. We observed remarkably few single nucleotide polymorphisms in the evolved strain, among which we confirmed a single amino acid change in the hexose transporter HXT7 coding sequence to be responsible for the evolved phenotype. Lastly, the mutant HXT7(F79S) shows improved xylose uptake rates (Vmax = 186.4 ± 20.1 nmol•min -1•mg -1) that allows the S. cerevisiae strain to show significant growth with xylose as the sole carbon source, as well as partial co-utilization of glucose and xylose in a mixed sugar cultivation.« less

Identification and proteomic analysis of a novel gossypol-degrading fungal strain.

PubMed

Yang, Xia; Sun, Jian-Yi; Guo, Jian-Lin; Weng, Xiao-Yan

2012-03-15

Cottonseed meal, an important source of feed raw materials, has limited use in the feed industry because of the presence of the highly toxic gossypol. The aim of the current work was to isolate the gossypol-degrading fungus from a soil microcosm and investigate the proteins involved in gossypol degradation. A fungal strain, AN-1, that uses gossypol as its sole carbon source was isolated and identified as Aspergillus niger. A large number of intracellular proteins were detected using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, but no significant difference was observed between the glucose-containing and gossypol-containing mycelium extracts. Two-dimensional gel electrophoresis results showed that the protein spots were concentrated in the 25.0-66.2 kDa range and distributed in different pI gradients. PDQuest software showed that 51 protein spots in the gels were differentially expressed. Of these, 20 differential protein spots, including six special spots expressed in gossypol, were analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The fungus AN-1 biodegraded gossypol and the proteomic analysis results indicate that some proteins were involved in the gossypol biodegradation during fungus survival, using gossypol as its sole carbon source. Copyright © 2011 Society of Chemical Industry.

Evolved hexose transporter enhances xylose uptake and glucose/xylose co-utilization in Saccharomyces cerevisiae

DOE PAGES

Reider Apel, Amanda; Ouellet, Mario; Szmidt-Middleton, Heather; ...

2016-01-19

Enhancing xylose utilization has been a major focus in Saccharomyces cerevisiae strain-engineering efforts. The incentive for these studies arises from the need to use all sugars in the typical carbon mixtures that comprise standard renewable plant-biomass-based carbon sources. While major advances have been made in developing utilization pathways, the efficient import of five carbon sugars into the cell remains an important bottleneck in this endeavor. Here we use an engineered S. cerevisiae BY4742 strain, containing an established heterologous xylose utilization pathway, and imposed a laboratory evolution regime with xylose as the sole carbon source. We obtained several evolved strains withmore » improved growth phenotypes and evaluated the best candidate using genome resequencing. We observed remarkably few single nucleotide polymorphisms in the evolved strain, among which we confirmed a single amino acid change in the hexose transporter HXT7 coding sequence to be responsible for the evolved phenotype. Lastly, the mutant HXT7(F79S) shows improved xylose uptake rates (Vmax = 186.4 ± 20.1 nmol•min -1•mg -1) that allows the S. cerevisiae strain to show significant growth with xylose as the sole carbon source, as well as partial co-utilization of glucose and xylose in a mixed sugar cultivation.« less

A Novel Denitrifying Extreme Halophile That Grows in a Simple Mineral Salts Medium

NASA Technical Reports Server (NTRS)

Hochstein, L. I.; Oremland, R. S.; Gherna, R.; Cote, R.; Chang, Sherwood (Technical Monitor)

1995-01-01

An extremely halophilic bacterium (strain CH-1) was isolated from a saltern adjacent to San Francisco Bay. It grew in a mineral salts medium with ammonium and glucose as sole sources of nitrogen and carbon as well as energy, respectively Cells lysed at less than 10% NaCl and growth was most rapid in medium containing 20% NaCl. Cells were pieomorphic ranging from disc to ovoid-shaved and used a variety of carbohydrates as sole carbon sources. the utilization of certain carbon sources was controlled by temperature with some used at 37 degrees but not 45 C. CH-1 grew between 30 degrees and 50 C with the optimum at 45 C in the presence of 20% NaCl. CH-1 contained 2,3-di-O-isoprenyl glcerol diethers and was sensitive to aphidicofin. The major polar lipid was glucosyl-mannosyl-alucosyl diether, which is diagnostic of the Haloarcula. Thus CH-1 is an extreme halophile and a member of this genus. Among the novel characteristics of this organism was its ability to grow anaerobically in synthetic medium when nitrate was present which was only reduced to nitrous oxide. This organism should prove useful for studying denitrification and carbohydrate metabolism in the extreme halophiles; and to be a valuable resource for generic studies.

Learning Analytics in Higher Education Development: A Roadmap

ERIC Educational Resources Information Center

Adejo, Olugbenga; Connolly, Thomas

2017-01-01

The increase in education data and advance in technology are bringing about enhanced teaching and learning methodology. The emerging field of Learning Analytics (LA) continues to seek ways to improve the different methods of gathering, analysing, managing and presenting learners' data with the sole aim of using it to improve the student learning…

Syphilis as a Sole Indicator of Sexual Abuse: Two Cases with No Intervention.

ERIC Educational Resources Information Center

Horowitz, Susan; Chadwick, David L.

1990-01-01

Two cases of suspected sexual abuse of five-year-old children with syphilis are reported. Lack of confirmation of abuse by either child or in parental interviews led to closing of the cases by Child Protection Services and continued residence by the children in their original homes. (DB)

49 CFR 1242.23 - Shop buildings-freight cars (account XX-13-25).

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 9 2013-10-01 2013-10-01 false Shop buildings-freight cars (account XX-13-25). 1242.23 Section 1242.23 Transportation Other Regulations Relating to Transportation (Continued) SURFACE... Structures § 1242.23 Shop buildings—freight cars (account XX-13-25). These accounts pertain solely to freight...

49 CFR 1242.23 - Shop buildings-freight cars (account XX-13-25).

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 9 2010-10-01 2010-10-01 false Shop buildings-freight cars (account XX-13-25). 1242.23 Section 1242.23 Transportation Other Regulations Relating to Transportation (Continued) SURFACE... Structures § 1242.23 Shop buildings—freight cars (account XX-13-25). These accounts pertain solely to freight...

49 CFR 1242.23 - Shop buildings-freight cars (account XX-13-25).

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 9 2011-10-01 2011-10-01 false Shop buildings-freight cars (account XX-13-25). 1242.23 Section 1242.23 Transportation Other Regulations Relating to Transportation (Continued) SURFACE... Structures § 1242.23 Shop buildings—freight cars (account XX-13-25). These accounts pertain solely to freight...

49 CFR 1242.23 - Shop buildings-freight cars (account XX-13-25).

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 9 2014-10-01 2014-10-01 false Shop buildings-freight cars (account XX-13-25). 1242.23 Section 1242.23 Transportation Other Regulations Relating to Transportation (Continued) SURFACE... Structures § 1242.23 Shop buildings—freight cars (account XX-13-25). These accounts pertain solely to freight...

49 CFR 1242.23 - Shop buildings-freight cars (account XX-13-25).

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 9 2012-10-01 2012-10-01 false Shop buildings-freight cars (account XX-13-25). 1242.23 Section 1242.23 Transportation Other Regulations Relating to Transportation (Continued) SURFACE... Structures § 1242.23 Shop buildings—freight cars (account XX-13-25). These accounts pertain solely to freight...

Children's Gestures and the Embodied Knowledge of Geometry

ERIC Educational Resources Information Center

Kim, Mijung; Roth, Wolff-Michael; Thom, Jennifer

2011-01-01

There is mounting research evidence that contests the metaphysical perspective of knowing as mental process detached from the physical world. Yet education, especially in its teaching and learning practices, continues to treat knowledge as something that is necessarily and solely expressed in ideal verbal form. This study is part of a funded…

26 CFR 301.7701(b)-2 - Closer connection exception.

Code of Federal Regulations, 2010 CFR

2010-04-01

... term “tax home” has the same meaning that it has for purposes of section 162(a)(2) (relating to travel... individual's tax home is the individual's regular place of abode in a real and substantial sense. (2.... It is material, however, that the dwelling be available at all times, continuously, and not solely...

Psychological Measurement Needs Units, Ratios, and Real Quantities: A Commentary on Humphry

ERIC Educational Resources Information Center

Kyngdon, Andrew

2011-01-01

Behavioral scientists have struggled with units of measurement for as long as they have struggled with measurement itself. Psychology's sole attempt at an explicit unit of measurement--the Lexile Framework for Reading (Stenner, Burdick, Sanford, & Burdick, 2006)--has been and continues to be ignored by the psychometric "cognoscenti."…

Implementation of Positive Behavior Intervention and Supports in Louisiana

ERIC Educational Resources Information Center

Hill, Juanita Mathis

2013-01-01

Many teachers have expressed their concern about continuous classroom disruption. Time taken to correct undesired behaviors is reducing the number of instructional minutes in the classroom on a daily basis. Instead of relying solely on classroom rules, the teacher who wishes to implement Positive Behavior Intervention and Supports should use and…

42 CFR 436.111 - Individuals who are not eligible for cash assistance because of a requirement not applicable...

Code of Federal Regulations, 2010 CFR

2010-10-01

... CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL... AFDC solely because of policies requiring the deeming of income and resources of the following...: (i) Stepparents who are not legally liable for support of stepchildren under a State law of general...

75 FR 8352 - Agency Information Collection Activities: Final Collection; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-24

... utilization and management prospective insurance liability relative to risk premiums received. DATES: Comments...). SUMMARY: The Export-Import Bank of the United States (Ex-Im Bank), as a part of its continuing effort to... Institution'' as an option; b. Under ``Obligo Types'' we have deleted ``Eximbank Sole Risk'' as an option; c...

21 CFR 558.365 - Nequinate.

Code of Federal Regulations, 2010 CFR

2010-04-01

... used as follows: (1) Broiler or fryer chickens—(i) Amount per ton. Nequinate, 18.16 grams. (ii...) Roaster chickens or replacement chickens for caged layers—(i) Amount per ton. Nequinate, 18.16 grams (0... continuously as the sole ration; do not feed to chickens over 16 weeks of age. [40 FR 13959, Mar. 27, 1975, as...

46 CFR 42.05-40 - Great Lakes.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 2 2012-10-01 2012-10-01 false Great Lakes. 42.05-40 Section 42.05-40 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) LOAD LINES DOMESTIC AND FOREIGN VOYAGES BY SEA... North America. (b) As used in this part, the term solely navigating the Great Lakes includes any special...

46 CFR 42.05-40 - Great Lakes.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 2 2014-10-01 2014-10-01 false Great Lakes. 42.05-40 Section 42.05-40 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) LOAD LINES DOMESTIC AND FOREIGN VOYAGES BY SEA... North America. (b) As used in this part, the term solely navigating the Great Lakes includes any special...

46 CFR 42.05-40 - Great Lakes.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 2 2013-10-01 2013-10-01 false Great Lakes. 42.05-40 Section 42.05-40 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) LOAD LINES DOMESTIC AND FOREIGN VOYAGES BY SEA... North America. (b) As used in this part, the term solely navigating the Great Lakes includes any special...

31 CFR 353.47 - Authorized reissue-during lifetime.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 31 Money and Finance:Treasury 2 2012-07-01 2012-07-01 false Authorized reissue-during lifetime. 353.47 Section 353.47 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued... are parties to a divorce or annulment; or (iii) The new sole owner is the trustee of a personal trust...

26 CFR 26.2653-1 - Taxation of multiple skips.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 26 Internal Revenue 14 2011-04-01 2010-04-01 true Taxation of multiple skips. 26.2653-1 Section 26.2653-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) ESTATE AND... Taxation of multiple skips. (a) General rule. If property is held in trust immediately after a GST, solely...

Efficient azo dye decolorization in a continuous stirred tank reactor (CSTR) with built-in bioelectrochemical system.

PubMed

Cui, Min-Hua; Cui, Dan; Gao, Lei; Cheng, Hao-Yi; Wang, Ai-Jie

2016-10-01

A continuous stirred tank reactor with built-in bioelectrochemical system (CSTR-BES) was developed for azo dye Alizarin Yellow R (AYR) containing wastewater treatment. The decolorization efficiency (DE) of the CSTR-BES was 97.04±0.06% for 7h with sludge concentration of 3000mg/L and initial AYR concentration of 100mg/L, which was superior to that of the sole CSTR mode (open circuit: 54.87±4.34%) and the sole BES mode (without sludge addition: 91.37±0.44%). The effects of sludge concentration and sodium acetate (NaAc) concentration on azo dye decolorization were investigated. The highest DE of CSTR-BES for 4h was 87.66±2.93% with sludge concentration of 12,000mg/L, NaAc concentration of 2000mg/L and initial AYR concentration of 100mg/L. The results in this study indicated that CSTR-BES could be a practical strategy for upgrading conventional anaerobic facilities against refractory wastewater treatment. Copyright © 2016 Elsevier Ltd. All rights reserved.

[Safety and efficacy of percutaneous patent ductus arteriosus closure solely under thoracic echocardiography guidance].

PubMed

Pan, Xiangbin; Ouyang, Wenbin; Li, Shoujun; Guo, Gaili; Liu, Yao; Zhang, Dawei; Zhang, Fengwen; Pang, Kunjing; Fang, Nengxin; Hu, Shengshou

2015-01-01

To avoid the radiation injuries and use of contrast agent, we assessed the safety and efficacy of percutaneous patent ductus arteriosus closure solely under thoracic echocardiography guidance. From June 2013 to June 2014, thirty patients (mean age: (6.3 ± 2.5) years, mean body weight:(22.5 ± 7.3) kg) with pure patent ductus arteriosus were continuously included in this study. The mean diameter of patent ductus arteriosus was (3.8 ± 0.9) mm. Patients were all treated by percutaneous patent ductus arteriosus closure via right femoral artery solely under thoracic echocardiography guidance. The efficacy of the procedure was evaluated by thoracic echocardiography. Follow-up was performed at one month after procedure. All 30 cases were successfully treated with percutaneous patent ductus arteriosus closure solely under thracic echocardiography guidance. The procedural time was (32.8 ± 5.7) minutes. The mean diameter of Amplatzer ADO II was (4.9 ± 1.0) mm. Postoperative trivial residual shunt occurred in six patients immediately after the procedure. All patients survived without peripheral vascular injury or complications such as cardiac perforation. Hospitalization time was (3.4 ± 0.7) days. At one-month follow-up, no complications such as residual shunt or pericardial effusion were observed. Echocardiography guided percutaneous patent ductus arteriosus closure by femoral artery approach is safe and effective, and can avoid X-ray and the use of contrast agents.

Development of a hybrid molecular beam epitaxy deposition system for in situ surface x-ray studies

NASA Astrophysics Data System (ADS)

Andersen, Tassie K.; Cook, Seyoung; Benda, Erika; Hong, Hawoong; Marks, Laurence D.; Fong, Dillon D.

2018-03-01

A portable metalorganic gas delivery system designed and constructed to interface with an existing molecular beam epitaxy chamber at beamline 33-ID-E of the Advanced Photon Source is described. This system offers the ability to perform in situ X-ray measurements of complex oxide growth via hybrid molecular beam epitaxy. The performance of the hybrid molecular beam epitaxy system while delivering metalorganic source materials is described. The high-energy X-ray scattering capabilities of the hybrid molecular beam epitaxy system are demonstrated both on oxide films grown solely from the metalorganic source and ABO3 oxide perovskites containing elements from both the metalorganic source and a traditional effusion cell.

Urease Activity Represents an Alternative Pathway for Mycobacterium tuberculosis Nitrogen Metabolism

PubMed Central

Lin, Wenwei; Mathys, Vanessa; Ang, Emily Lei Yin; Koh, Vanessa Hui Qi; Martínez Gómez, Julia María; Ang, Michelle Lay Teng; Zainul Rahim, Siti Zarina; Tan, Mai Ping; Pethe, Kevin

2012-01-01

Urease represents a critical virulence factor for some bacterial species through its alkalizing effect, which helps neutralize the acidic microenvironment of the pathogen. In addition, urease serves as a nitrogen source provider for bacterial growth. Pathogenic mycobacteria express a functional urease, but its role during infection has yet to be characterized. In this study, we constructed a urease-deficient Mycobacterium tuberculosis strain and confirmed the alkalizing effect of the urease activity within the mycobacterium-containing vacuole in resting macrophages but not in the more acidic phagolysosomal compartment of activated macrophages. However, the urease-mediated alkalizing effect did not confer any growth advantage on M. tuberculosis in macrophages, as evidenced by comparable growth profiles for the mutant, wild-type (WT), and complemented strains. In contrast, the urease-deficient mutant exhibited impaired in vitro growth compared to the WT and complemented strains when urea was the sole source of nitrogen. Substantial amounts of ammonia were produced by the WT and complemented strains, but not with the urease-deficient mutant, which represents the actual nitrogen source for mycobacterial growth. However, the urease-deficient mutant displayed parental colonization profiles in the lungs, spleen, and liver in mice. Together, our data demonstrate a role for the urease activity in M. tuberculosis nitrogen metabolism that could be crucial for the pathogen's survival in nutrient-limited microenvironments where urea is the sole nitrogen source. Our work supports the notion that M. tuberculosis virulence correlates with its unique metabolic versatility and ability to utilize virtually any carbon and nitrogen sources available in its environment. PMID:22645285

CANCER MORTALITY AMONG UNITED STATES COUNTIES WITH HAZARDOUS WASTE SITES AND SOLE SOURCE DRINKING WATER CONTAMINATION

EPA Science Inventory

Since the late 1950's more than 750 million tons of toxic wastes have been discarded in an estimated 30,000 to 50,000 hazardous waste sies (HWS). he uncontrolled discarding of chemical wastes creates the potential for risks to human health. tilizing the National Priorities Listin...

Publicizing Your Web Resources for Maximum Exposure.

ERIC Educational Resources Information Center

Smith, Kerry J.

2001-01-01

Offers advice to librarians for marketing their Web sites on Internet search engines. Advises against relying solely on spiders and recommends adding metadata to the source code and delivering that information directly to the search engines. Gives an overview of metadata and typical coding for meta tags. Includes Web addresses for a number of…

Complete genome sequence of a carbon monoxide-utilizing acetogen, Eubacterium limosum KIST612.

PubMed

Roh, Hanseong; Ko, Hyeok-Jin; Kim, Daehee; Choi, Dong Geon; Park, Shinyoung; Kim, Sujin; Chang, In Seop; Choi, In-Geol

2011-01-01

Eubacterium limosum KIST612 is an anaerobic acetogenic bacterium that uses CO as the sole carbon/energy source and produces acetate, butyrate, and ethanol. To evaluate its potential as a syngas microbial catalyst, we have sequenced the complete 4.3-Mb genome of E. limosum KIST612.

40 CFR 419.34 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2010 CFR

2010-07-01

... limitations for contaminated runoff. The following effluent limitations constitute the quantity and quality of pollutants or pollutant properties controlled by this paragraph and attributable to contaminated runoff which... point source subject to this subpart. (1) If wastewater consists solely of contaminated runoff and is...

40 CFR 149.105 - Decision to review.

Code of Federal Regulations, 2012 CFR

2012-07-01

... Sole Source Aquifer in the San Antonio, Texas Area § 149.105 Decision to review. (a) The Regional... of the aquifer for which a draft or final EIS is submitted which may have an impact on ground water... of a public petition, the Regional Administrator shall decide whether the project which is the...

40 CFR 149.105 - Decision to review.

Code of Federal Regulations, 2013 CFR

2013-07-01

... Sole Source Aquifer in the San Antonio, Texas Area § 149.105 Decision to review. (a) The Regional... of the aquifer for which a draft or final EIS is submitted which may have an impact on ground water... of a public petition, the Regional Administrator shall decide whether the project which is the...

40 CFR 149.105 - Decision to review.

Code of Federal Regulations, 2014 CFR

2014-07-01

... Sole Source Aquifer in the San Antonio, Texas Area § 149.105 Decision to review. (a) The Regional... of the aquifer for which a draft or final EIS is submitted which may have an impact on ground water... of a public petition, the Regional Administrator shall decide whether the project which is the...

48 CFR 19.307 - Protesting a firm's status as a service-disabled veteran-owned small business concern.

Code of Federal Regulations, 2012 CFR

2012-10-01

... as a service-disabled veteran-owned small business concern. 19.307 Section 19.307 Federal Acquisition...-disabled veteran-owned small business concern. (a) For sole source acquisitions, the SBA or the contracting officer may protest the apparently successful offeror's service-disabled veteran-owned small business...

48 CFR 19.307 - Protesting a firm's status as a service-disabled veteran-owned small business concern.

Code of Federal Regulations, 2011 CFR

2011-10-01

... as a service-disabled veteran-owned small business concern. 19.307 Section 19.307 Federal Acquisition...-disabled veteran-owned small business concern. (a) For sole source acquisitions, the SBA or the contracting officer may protest the apparently successful offeror's service-disabled veteran-owned small business...

48 CFR 19.307 - Protesting a firm's status as a service-disabled veteran-owned small business concern.

Code of Federal Regulations, 2013 CFR

2013-10-01

... as a service-disabled veteran-owned small business concern. 19.307 Section 19.307 Federal Acquisition...-disabled veteran-owned small business concern. (a) For sole source acquisitions, the SBA or the contracting officer may protest the apparently successful offeror's service-disabled veteran-owned small business...

Can an increase in leaf iron reductase activity enhance seed iron accumulation in soybean?

USDA-ARS?s Scientific Manuscript database

Iron is an important micronutrient for human nutrition, with plant foods providing a significant amount of dietary iron in certain population groups, and in some cases, providing the sole source of dietary iron. Because iron deficiency is unfortunately common in many human populations, we have been...

EFFECTS ON THE FETAL RAT INTESTINE OF MATERNAL MALNUTRITION AND EXPOSURE TO NITROFEN (2,4-DICHLOROPHENYL-P-NITROPHENYL ETHER)

EPA Science Inventory

The effects of maternal protein-energy malnutrition and exposure to nitrofen on selected aspects of intestinal morphology and function were studied in the fetal rat. Pregnant rats were fed, throughout gestation, diets containing 24% or 6% casein as the sole source of protein. Red...

Propagating native milkweeds for restoring monarch butterfly habitat

Treesearch

Thomas D. Landis; R. Kasten Dumroese

2015-01-01

The number of monarch butterflies, charismatic nomads of North America, is rapidly declining. Milkweeds (Asclepias spp.), which are the sole food source for monarch caterpillars, have also experienced a decline throughout the breeding range of this butterfly. Milkweeds can be grown from seeds or vegetatively from root cuttings or rhizomes. Seed germination is often...

Classes of Legitimate Evidence for Identifying Effective Teaching.

ERIC Educational Resources Information Center

Wagner, Paul A.

A criterion for selecting sources of evidence to evaluate effective teaching is described. It is suggested that teaching effectiveness is not measured solely in terms of cognitive change in students but in the extent to which academics practice teaching in accordance with the moral dictates of the profession. In developing a teacher effectiveness…

Novel ferulate esterase from Gram-positive lactic acid bacteria and analyses of the recombinant enzyme produced in E. coli

USDA-ARS?s Scientific Manuscript database

Using a plate containing ethyl ferulate as sole carbon source, various bacteria cultures were screened for ferulate esterase (FAE). Among a dozen of species showing positive FAE, one Lactobacillus fermentum strain NRRL 1932 demonstrated the strongest activity. Using a published sequence of ferulate ...

Novel feruloyl esterase from Lactobacillus fermentum NRRL B-1932 and analysis of the recombinant enzyme produced in Escherichia coli.

USDA-ARS?s Scientific Manuscript database

Using agar plates containing ethyl ferulate as the sole carbon source, 33 Lactobacillus strains were screened for feruloyl esterase (FE) activity. Among a dozen species showing a clearing zone on the opaque plate containing ethyl ferulate, Lactobacillus fermentum NRRL B-1932 demonstrated the stronge...

Seasonal habitat associations of the wolverine in central Idaho

Treesearch

Jeffrey P. Copeland; James M. Peek; Craig R. Groves; Wayne E. Melquist; Kevin S. Mckelvey; Gregory W. McDaniel; Clinton D. Long; Charles E. Harris

2007-01-01

Although understanding habitat relationships remains fundamental to guiding wildlife management, these basic prerequisites remain vague and largely unstudied for the wolverine. Currently, a study of wolverine ecology conducted in Montana, USA, in the 1970s is the sole source of information on habitat requirements of wolverines in the conterminous United States. The...

A Culturally Sensitive Analysis of Culture in the Context of Context: When Is Enough Enough?

ERIC Educational Resources Information Center

Kahn, Peter H., Jr.

Cultural context is not the sole source of human knowledge. Postmodern theory, in both its deconstructionist and affirmative approaches, offers an incomplete basis by which to study race, class, and gender, and undermines ethical interaction. Deconstructionism calls for the abandonment of generalizable research findings, asserting that the concept…

18 CFR 292.602 - Exemption to qualifying facilities from the Public Utility Holding Company Act of 2005 and...

Code of Federal Regulations, 2014 CFR

2014-04-01

... PRODUCTION AND COGENERATION Exemption of Qualifying Small Power Production Facilities and Cogeneration... small power production facility with a power production capacity over 30 megawatts if such facility produces electric energy solely by the use of biomass as a primary energy source. (b) Exemption from the...