RNA interference of pax2 inhibits growth of transplanted human endometrial cancer cells in nude mice

PubMed Central

Zhang, Li-Ping; Shi, Xiao-Yan; Zhao, Chang-Yin; Liu, Yong-Zhen; Cheng, Ping

2011-01-01

The development of human endometrial carcinoma (HEC) is a complex pathologic process involves several oncogenes and tumor suppressor genes. The full-length paired-box gene 2 (pax2), a recently discovered Oncogene, promotes cell proliferation and growth and inhibits apoptosis of HEC cells. Here, we examined the effect of pax2 small interfering RNA (siRNA) on the growth of transplanted HEC cells in nude mice. The expression of Pax2 in 21 cases of normal endometrium and 38 cases of HEC was examined by immohistochemistry (IHC). HEC models were developed by subcutaneously transferring HEC cells into nude mice, followed by treatment with empty lentivirus vector, lentivirus vector-based pax2 siRNA, and phosphate buffered saline, respectively. Four weeks later, tumor size was measured, tumor inhibition rate was calculated, and histological analyses were conducted after staining with hematoxylin and eosin. The expression of Pax2 and Bcl-2 was detected by Western blot; proliferating cell nuclear antigen (PCNA) was detected by IHC. Significant differences were observed in the positive rate of Pax2 between normal endometrium and HEC (14.2% vs. 60.5%, P < 0.01). The expression index of Pax2 in well differentiated tumors was 1.88 ± 1.68, much lower than that in tumors of moderate (3.07 ± 1.96, P < 0.05) or poor differentiation (5.45 ± 2.76, P < 0.01). Tumor necrosis increased, nuclear basophilia stain decreased, tumor growth was inhibited, and PCNA, Pax2, and Bcl-2 expression was reduced in HEC models treated with pax2 siRNA. These results indicate that Pax2 expression is related to HEC tumor biology with the increased expression of Pax2 correlated to malignancy. pax2 siRNA down-regulates Pax2 expression and inhibits tumorigenesis of HEC in nude mice, possibly due to cell apoptosis and the inhibition of tumor proliferation induced by down-regulation of Bcl-2. PMID:21627862

Effects of dexamethasone-21-isonicotinate on peripheral insulin action in dairy cows 5 days after surgical correction of abomasal displacement.

PubMed

Kusenda, M; Kaske, M; Piechotta, M; Locher, L; Starke, A; Huber, K; Rehage, J

2013-01-01

Dexamethasone frequently is used for treatment of ketosis in dairy cows, but its effects are not fully understood. Dexamethasone treatment affects whole body insulin sensitivity. Twelve German Holstein cows, 2-4 weeks postpartum, 5 days after omentopexy to correct left abomasal displacement. Randomized, blinded, case-control study. Treatment with dexamethasone-21-isonicotinate (DG; 40 μg/kg IM; n = 6) or saline (control group [CG], 15 mL IM, n = 6) on day 0 (d0). Blood samples were obtained before (d0) and after treatment (d1 and d2), and analyzed for glucose, insulin, and nonesterified fatty acid (NEFA) concentrations. Hepatic triglycerides (TAG) were measured in liver samples taken on d0 and d2. Five consecutive hyperinsulinemic-euglycemic clamps (HEC-I-V; insulin dosages: 0.1, 0.5, 2, 5, 10 mU/kg/min, respectively) were performed on d1 and steady state glucose infusion rate (SSGIR), insulin concentration (SSIC), insulin sensitivity index (ISI = SSGIR/SSIC), and plasma NEFA concentration (SSNEFA) were assessed. Compared with CG-cows, DG-cows on d1 had higher plasma glucose (P = .004) and insulin (P < .001) concentrations, decreased SSGIR (HEC-II, P = .002; HEC-IV, P = .033), ISI (HEC-I, P < .015; HEC-II, P = .004), and insulin-stimulated decrease in SSNEFA (HEC-II, P = .006; HEC-III, P = .01; HEC-IV, P = .003; HEC-V, P = .011). Decrease in hepatic TAG content in DG-cows was higher compared with CG-cows (P < .001). Dexamethasone decreases whole body insulin sensitivity and affects glucose and lipid metabolism in early lactating dairy cows. Copyright © 2012 by the American College of Veterinary Internal Medicine.

Moral Reasoning among HEC Members: An Empirical Evaluation of the Relationship of Theory and Practice in Clinical Ethics Consultation.

PubMed

Wasserman, Jason Adam; Stevenson, Shannon Lindsey; Claxton, Cassandra; Krug, Ernest F

2015-01-01

In light of the ongoing development and implementation of core competencies in bioethics, it is important to proceed with a clear sense of how bioethics knowledge is utilized in the functioning of hospital ethics committees (HECs). Without such an understanding, we risk building a costly edifice on a foundation that is ambiguous at best. This article examines the empirical relationship between traditional paradigms of bioethics theory and actual decision making by HEC members using survey data from HEC members. The assumption underlying the standardization of qualifications and corresponding call for increased education of HEC members is that they will base imminent case decisions on inculcated knowledge. Our data suggest, however, that HEC members first decide intuitively and then look for justification, thereby highlighting the need to re-examine the pedagogical processes of ethics education in the process of standardizing and improving competencies. Copyright 2015 The Journal of Clinical Ethics. All rights reserved.

The Evolution of American Hospital Ethics Committees: A Systematic Review.

PubMed

Courtwright, Andrew; Jurchak, Martha

2016-01-01

During the 1970s and 1980s, legal precedent, governmental recommendations, and professional society guidelines drove the formation of hospital ethics committees (HECs). The Joint Commission on Accreditation of Health Care Organization's requirements in the early 1990s solidified the role of HECs as the primary mechanism to address ethical issues in patient care. Because external factors drove the rapid growth of HECs on an institution-by-institution basis, however, no initial consensus formed around the structure and function of these committees. There are now almost 40 years of empirical studies on the composition, administration, and activities of HECs in the United States. We conducted a systematic review of the available empirical literature on HECs to describe their evolution. As HECs changed over time, they increased their total number of members and percentage of members from nursing and the community. Although physicians increasingly chaired these committees, their presence as a percentage of overall members declined. The percentage of administrative members remained steady, although committees became increasingly likely to have at least one administrative member. HECs were also increasingly likely to report to an administrative body or to the board of trustees or directors rather than to the medical staff. Finally, consultation volume increased steadily over time. There has not, however, been a national survey of the composition of ethics committees, their administration, or volume of consultation in more than 10 years, despite increasing calls for professional standards and quality improvement assessments among HECs. Copyright 2016 The Journal of Clinical Ethics. All rights reserved.

Using HEC-HMS: Application to Karkheh river basin

USDA-ARS?s Scientific Manuscript database

This paper aims to facilitate the use of HEC-HMS model using a systematic event-based technique for manual calibration of soil moisture accounting and snowmelt degree-day parameters. Manual calibration, which helps ensure the HEC-HMS parameter values are physically-relevant, is often a time-consumin...

Surface-modified anodic aluminum oxide membrane with hydroxyethyl celluloses as a matrix for bilirubin removal.

PubMed

Xue, Maoqiang; Ling, Yisheng; Wu, Guisen; Liu, Xin; Ge, Dongtao; Shi, Wei

2013-01-01

Microporous anodic aluminum oxide (AAO) membranes were modified by 3-glycidoxypropyltrimethoxysilane to produce terminal epoxy groups. These were used to covalently link hydroxyethyl celluloses (HEC) to amplify reactive groups of AAO membrane. The hydroxyl groups of HEC-AAO composite membrane were further modified with 1,4-butanediol diglycidyl ether to link arginine as an affinity ligand. The contents of HEC and arginine of arginine-immobilized HEC-AAO membrane were 52.1 and 19.7mg/g membrane, respectively. As biomedical adsorbents, the arginine-immobilized HEC-AAO membranes were tested for bilirubin removal. The non-specific bilirubin adsorption on the unmodified HEC-AAO composite membranes was 0.8mg/g membrane. Higher bilirubin adsorption values, up to 52.6mg/g membrane, were obtained with the arginine-immobilized HEC-AAO membranes. Elution of bilirubin showed desorption ratio was up to 85% using 0.3M NaSCN solution as the desorption agent. Comparisons equilibrium and dynamic capacities showed that dynamic capacities were lower than the equilibrium capacities. In addition, the adsorption mechanism of bilirubin and the effects of temperature, initial concentration of bilirubin, albumin concentration and ionic strength on adsorption were also investigated. Copyright © 2012 Elsevier B.V. All rights reserved.

Hydroxyethyl cellulose hydrogel for wound dressing: Fabrication, characterization and in vitro evaluation.

PubMed

El Fawal, Gomaa F; Abu-Serie, Marwa M; Hassan, Mohamed A; Elnouby, Mohamed S

2018-05-01

In this study, new hydrogel membranes were developed based on hydroxyethyl cellulose (HEC) supplemented with tungsten oxide for further implementing in wound treatment. HEC hydrogel membranes were fabricated and crosslinked using citric acid (CA). Various tests were carried out including FTIR, XRD, porosity measurements, swelling, mechanical properties, gel fraction, and thermal gravimetric analysis to evaluate the efficiency of the prepared membranes as wound dressing material. In addition, wound healing activity of the examined membranes for human dermal fibroblast cell line was investigated employing in vitro scratching model. Furthermore, the potency of the prepared membranes to suppress wound complications was studied via determination of their anti-inflammatory and antibacterial activities exploiting MTT, ELISA, and disk agar diffusion methods. The results demonstrated that the HEC hydrogel membranes revealed an anti-inflammatory and antibacterial efficacy. Moreover, HEC improved the safety of tungsten oxide toward normal human cells (white blood cells and dermal fibroblast). Furthermore, HEC membranes loaded with WO 3 revealed the highest activities against Salmonella sp. pursued by P. aeruginosa in compared with the negative HEC hydrogel membrane. The current approach corroborated that HEC amended by tungsten oxide could be applied as a promising safe candidate for wound dressing material. Copyright © 2018 Elsevier B.V. All rights reserved.

S-protected thiolated hydroxyethyl cellulose (HEC): Novel mucoadhesive excipient with improved stability.

PubMed

Leonaviciute, Gintare; Bonengel, Sonja; Mahmood, Arshad; Ahmad Idrees, Muneeb; Bernkop-Schnürch, Andreas

2016-06-25

The aim of this study was the design of novel S-protected thiolated hydroxyethyl cellulose (HEC) and the assessment of its mucoadhesive properties and biodegradability compared to the corresponding unmodified polymer. Thiolated HEC was S-protected via disulfide bond formation between 6-mercaptonicotinamide (6-MNA) and the thiol substructures of the polymer. In vitro screening of mucoadhesive properties was accomplished using two different methods: rotating cylinder studies and viscosity measurements. Moreover, biodegradability of these polymers by cellulase, xylanase and lysozyme was evaluated. MTT and LDH assays were performed on Caco-2 cells to determine the cytotoxicity of S-protected thiolated HEC. Thiolated HEC displayed 280.09±1.70μmol of free thiol groups per gram polymer. S-protected thiolated HEC exhibiting 270.8±21.11μmol immobilized 6-MNA ligands per gram of polymer was shown being 2.4-fold more mucoadhesive compared to thiolated HEC. No mucoadhesion was observed in case of unmodified HEC. Results were in a good agreement with rheological studies. The presence of free thiol moieties likely caused lower degree of hydrolysis by xylanase, whereas the degradation by both enzymes cellulase and xylanase was more hampered when 6-MNA was introduced as ligand for thiol group's protection. Findings in cell viability revealed that all three conjugates were non-toxic. S-protection of thiolated hydroxyethyl cellulose improved mucoadhesive properties and provided pronounced stability towards enzymatic attack, that makes this excipient superior for non-invasive drug administration over thiolated and unmodified forms. Copyright © 2016 Elsevier Ltd. All rights reserved.

Evolutionary processes and sources of high-nitrate haze episodes over Beijing, Spring.

PubMed

Yang, Ting; Sun, Yele; Zhang, Wei; Wang, Zifa; Liu, Xingang; Fu, Pingqing; Wang, Xiquan

2017-04-01

Rare and consecutive high-nitrate haze pollution episodes were observed in Beijing in spring 2012. We present detailed characterization of the sources and evolutionary mechanisms of this haze pollution, and focus on an episode that occurred between 15 and 26 April. Submicron aerosol species were found to be substantially elevated during haze episodes, and nitrates showed the largest increase and occupation (average: 32.2%) in non-refractory submicron particles (NR-PM 1 ), which did not occur in other seasons as previously reported. The haze episode (HE) was divided into three sub-episodes, HEa, HEb, and HEc. During HEa and HEc, a shallow boundary layer, stagnant meteorological conditions, and high humidity favored the formation of high-nitrate concentrations, which were mainly produced by three different processes - daytime photochemical production, gas-particle partitioning, and nighttime heterogeneous reactions - and the decline in visibility was mainly induced by NR-PM 1. However, unlike HEa and HEc, during HEb, the contribution of high nitrates was partly from the transport of haze from the southeast of Beijing - the transport pathway was observed at ~800-1000m by aerosol Lidar - and the decline in visibility during HEb was primarily caused by PM 2.5 . Our results provide useful information for air quality improvement strategies in Beijing during Spring. Copyright © 2016. Published by Elsevier B.V.

Arabidopsis HECATE genes function in phytohormone control during gynoecium development.

PubMed

Schuster, Christoph; Gaillochet, Christophe; Lohmann, Jan U

2015-10-01

The fruit, which develops from the fertilised gynoecium formed in the innermost whorl of the flower, is the reproductive organ and one of the most complex structures of an angiosperm plant. Phytohormones play important roles during flower and fruit patterning, morphogenesis and growth, and there is emerging evidence for a cross-talk between different classes of plant hormones throughout these processes. Here, we show that the bHLH transcription factors HECATE 1 (HEC1), HEC2 and HEC3, which have previously been identified as essential components of transmitting tract formation, affect both auxin and cytokinin responses during reproductive tissue development. We find that HEC1 interacts with SPATULA (SPT) to control carpel fusion and that both transcription factors restrict sensitivity to cytokinin in the gynoecium. In addition, HEC1 is tightly integrated into the auxin-signalling network at the levels of biosynthesis, transport and transcriptional response. Based on this data, we propose that HEC1 acts as a local modulator of auxin and cytokinin responses to control gynoecium development in Arabidopsis. © 2015. Published by The Company of Biologists Ltd.

A truncated form of CD9-partner 1 (CD9P-1), GS-168AT2, potently inhibits in vivo tumour-induced angiogenesis and tumour growth

PubMed Central

Colin, S; Guilmain, W; Creoff, E; Schneider, C; Steverlynck, C; Bongaerts, M; Legrand, E; Vannier, J P; Muraine, M; Vasse, M; Al-Mahmood, S

2011-01-01

Background: Tetraspanins are transmembrane proteins known to contribute to angiogenesis. CD9 partner-1 (CD9P-1/EWI-F), a glycosylated type 1 transmembrane immunoglobulin, is a member of the tetraspanin web, but its role in angiogenesis remains to be elucidated. Methods: We measured the expression of CD9P-1 under angiogenic and angiostatic conditions, and the influence of its knockdown onto capillary structures formation by human endothelial cells (hECs). A truncated form of CDP-1, GS-168AT2, was produced and challenged vs hEC proliferation, migration and capillaries' formation. Its association with CD9P-1, CD9, CD81 and CD151 and the expressions of these later at hEC surface were analysed. Finally, its effects onto in vivo tumour-induced angiogenesis and tumour growth were investigated. Results: Vascular endothelial growth factor (VEGF)-induced capillary tube-like formation was inhibited by tumour necrosis factor α and was associated with a rise in CD9P-1 mRNA expression (P<0.05); accordingly, knockdown of CD9P-1 inhibited VEGF-dependent in vitro angiogenesis. GS-168AT2 dose-dependently inhibited in vitro angiogenesis, hEC migration and proliferation (P<0.05). Co-precipitation experiments suggest that GS-168AT2 corresponds to the sequence by which CD9P-1 physiologically associates with CD81. GS-168AT2 induced the depletion of CD151, CD9 and CD9P-1 from hEC surface, correlating with GS-168AT2 degradation. Finally, in vivo injections of GS-168AT2 inhibited tumour-associated angiogenesis by 53.4±9.5% (P=0.03), and reduced tumour growth of Calu 6 tumour xenografts by 73.9±16.4% (P=0.007) without bodyweight loss. Conclusion: The truncated form of CD9P-1, GS-168AT2, potently inhibits angiogenesis and cell migration by at least the downregulation of CD151 and CD9, which provides the first evidences for the central role of CD9P-1 in tumour-associated angiogenesis and tumour growth. PMID:21863033

Acute Effects of Aerobic Exercise on Affect and Smoking Craving in the Weeks Before and After a Cessation Attempt.

PubMed

Abrantes, Ana M; Farris, Samantha G; Minami, Haruka; Strong, David R; Riebe, Deborah; Brown, Richard A

2018-04-02

Aerobic exercise may improve smoking abstinence via reductions in craving and negative affect and increases in positive moods. Acute changes in craving and affect before and after structured exercise sessions have not been examined during the weeks prior to and following quit attempts nor has smoking status been examined in relation to these effects. Given that regular cigarette smoking can be perceived as affect enhancing and craving reducing, it is not known whether exercise could contribute additional affective benefit beyond these effects. Participants (N = 57; 68.4% women) were low-active daily smokers randomized to cessation treatments plus either group-based aerobic exercise (AE) or a health-education control (HEC). Mood, anxiety, and craving were assessed before and after each intervention session for each of the 12 weeks. Carbon monoxide (CO) breath samples ≤ 5ppm indicated smoking abstinence. During the prequit sessions, significantly greater decreases in anxiety following AE sessions relative to HEC sessions were observed. Changes in mood and craving were similar after AE and HEC sessions prior to quitting. Postquit attempt, significant reductions in craving and anxiety were observed after AE sessions but not following HEC. During the postquit period, positive mood increased following AE sessions relative to HEC only among individuals who were abstinence on that day. AE may be effective in acutely reducing anxiety prior to a quit attempt and both anxiety and craving following the quit attempt regardless of abstinence status. The mood-enhancing effects of AE may occur only in the context of smoking abstinence. The current findings underscore the importance of examining the acute effects of aerobic exercise prior to and after a cessation attempt and as a function of smoking status. Given the equivocal results from previous studies on the efficacy of exercise for smoking cessation, increasing our understanding of how aerobic exercise produces its reinforcing benefits for smokers attempting to quit could potentially inform the refinement (e.g., timing/sequencing) of exercise interventions within smoking cessation programs.

Buried waste integrated demonstration human engineered control station. Final report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1994-09-01

This document describes the Human Engineered Control Station (HECS) project activities including the conceptual designs. The purpose of the HECS is to enhance the effectiveness and efficiency of remote retrieval by providing an integrated remote control station. The HECS integrates human capabilities, limitations, and expectations into the design to reduce the potential for human error, provides an easy system to learn and operate, provides an increased productivity, and reduces the ultimate investment in training. The overall HECS consists of the technology interface stations, supporting engineering aids, platform (trailer), communications network (broadband system), and collision avoidance system.

Hospital/clinical ethics committees' notion: an overview

PubMed Central

Hajibabaee, Fatemeh; Joolaee, Soodabeh; Cheraghi, Mohammad Ali; Salari, Pooneh; Rodney, Patricia

2016-01-01

Hospital ethics committees (HECs) help clinicians deal with the ethical challenges which have been raised during clinical practice. A comprehensive literature review was conducted to provide a historical background of the development of HECs internationally and describe their functions and practical challenges of their day to day work. This is the first part of a comprehensive literature review conducted between February 2014 and August 2016 by searching through scientific databases. The keyword ethics committee, combined with hospital, clinic, and institution, was used without a time limitation. All original and discussion articles, as well as other scientific documents were included. Of all the articles and theses found using these keywords, only 56 were consistent with the objectives of the study. Based on the review goals, the findings were divided into three main categories; the inception of HECs in the world, the function of HECs, and the challenges of HECs. According to the results, the Americas Region and European Region countries have been the most prominent considering the establishment of HECs. However, the majority of the Eastern Mediterranean Region and South-East Asia Region countries are only beginning to establish these committees in their hospitals. The results highlight the status and functions of HECs in different countries and may be used as a guide by health policymakers and managers who are at the inception of establishing these committees in their hospitals. PMID:28523118

Home-Made Money: Consumer's Guide to Home Equity Conversion.

ERIC Educational Resources Information Center

Scholen, Ken

This guide was written to introduce consumers to home equity conversion (HEC) plans that are currently available and to explain the current state of HEC developments. It describes how the basic types of HEC plans work, gives examples of how how they can be used, discusses their advantages and disadvantages, and tells where they are available.…

2D Hydrodynamic Investigation of Olmsted Cofferdams

DTIC Science & Technology

2013-07-01

34  Figure 30. HEC - RAS cross-section locations in the AdH 2012. ........................................................... 34...stress in Pascals for Condition 1 (typical). ........................................................ 40  Figure 43. HEC - RAS cross-section locations in...Then in 2008, CHL used an Adaptive Hydraulics model (AdH) to further evaluate potential impacts on mussel beds. In 2012, LRL used a HEC - RAS model to

Error of the modelled peak flow of the hydraulically reconstructed 1907 flood of the Ebro River in Xerta (NE Iberian Peninsula)

NASA Astrophysics Data System (ADS)

Lluís Ruiz-Bellet, Josep; Castelltort, Xavier; Carles Balasch, J.; Tuset, Jordi

2016-04-01

The estimation of the uncertainty of the results of the hydraulic modelling has been deeply analysed, but no clear methodological procedures as to its determination have been formulated when applied to historical hydrology. The main objective of this study was to calculate the uncertainty of the resulting peak flow of a typical historical flood reconstruction. The secondary objective was to identify the input variables that influenced the result the most and their contribution to peak flow total error. The uncertainty of 21-23 October 1907 flood of the Ebro River (NE Iberian Peninsula) in the town of Xerta (83,000 km2) was calculated with a series of local sensitivity analyses of the main variables affecting the resulting peak flow. Besides, in order to see to what degree the result depended on the chosen model, the HEC-RAS resulting peak flow was compared to the ones obtained with the 2D model Iber and with Manning's equation. The peak flow of 1907 flood in the Ebro River in Xerta, reconstructed with HEC-RAS, was 11500 m3·s-1 and its total error was ±31%. The most influential input variable over HEC-RAS peak flow results was water height; however, the one that contributed the most to peak flow error was Manning's n, because its uncertainty was far greater than water height's. The main conclusion is that, to ensure the lowest peak flow error, the reliability and precision of the flood mark should be thoroughly assessed. The peak flow was 12000 m3·s-1 when calculated with the 2D model Iber and 11500 m3·s-1 when calculated with the Manning equation.

Nurses' knowledge, attitudes and willingness to participate officially in workplace Healthcare Ethics Committees (HEC).

PubMed

Rubinstein, Dorit; Tabak, Nili

2012-03-01

This research was designed to assess nurses' perceptions, knowledge, attitudes and intentions in relation to nurse participation in Healthcare Ethics Committees (HECs). A convenience sample of 87 nurses from five Israeli hospitals completed a self-administered questionnaire, whose data were then analyzed by quantitative statistics. The main findings were that large percentages of nurses were totally ignorant of the existence and functioning of the HEC in their workplaces. Nurses in managerial roles were (a) much more knowledgeable on these matters than staff nurses and (b) regarded more positively the idea that nurses had an obligation to sit on such committees. Workplace role and rank in the organizational hierarchy had a stronger impact on nurse attitudes to HEC work than level of education. Overall, nurse willingness to sit on an HEC and to take special training in preparation for such a role were high.

Design of cellulose ether-based macromolecular prodrugs of ciprofloxacin for extended release and enhanced bioavailability.

PubMed

Amin, Muhammad; Abbas, Nazia Shahana; Hussain, Muhammad Ajaz; Sher, Muhammad; Edgar, Kevin J

2018-07-01

The present study reveals the syntheses of hydroxypropylcellulose‑(HPC) and hydroxyethylcellulose‑(HEC) based macromolecular prodrugs (MPDs) of ciprofloxacin (CIP) using homogeneous reaction methodology. Covalently loaded drug content (DC) of each prodrug was quantified using UV-Vis spectrophotometry to determine degree of substitution (DS). HPC-ciprofloxacin (HPC-CIP) conjugates showed DS of CIP in the range 0.87-1.15 whereas HEC-ciprofloxacin (HEC-CIP) conjugates showed DS range 0.51-0.75. Transmission electron microscopy revealed that HPC-CIP conjugate 2 and HEC-CIP conjugate 6 self-assembled into nanoparticles of 150-300 and 180-250nm, respectively. Size exclusion chromatography revealed HPC-CIP conjugate 2 and HEC-CIP conjugate 6 as monodisperse systems. In vitro drug release studies indicated 15 and 43% CIP release from HPC-CIP conjugate 2 after 6h in simulated gastric and simulated intestinal fluids (SGF and SIF), respectively. HEC-CIP conjugate 6 showed 16% and 46% release after 6h in SGF and SIF, respectively. HPC-CIP conjugate 2 and HEC-CIP conjugate 6 exhibited half-lives of 10.87 and 11.71h, respectively with area under the curve values of 164 and 175hμgmL -1 , respectively, indicating enhanced bioavailability and improved pharmacokinetic profiles in animal model. Equal antibacterial activities to that of unmodified CIP confirmed their competitive efficacies. Cytotoxicity studies supported their non-toxic nature and biocompatibility. Copyright © 2018 Elsevier B.V. All rights reserved.

Mount St. Helens Future Expected Deposition Scenario (FEDS)

DTIC Science & Technology

2011-04-14

Gradation in HEC - RAS Sediment Transport Model of the Lower Cowlitz River ...Cowlitz 1-D/2-D modeling. Will also be used to test proposed measures where appropriate. Cowlitz River Toutle to Columbia 1-D HEC - RAS Aug 2004...Sep 2008 (6 years) Calibration Model Cowlitz River Toutle to Columbia 1-D HEC - RAS Oct 2007 – Sep 2035 (28 years) Forecast to predict trends in

Model Integration for Real-Time Flood Forecasting Inundation Mapping for Nashville Tributaries

NASA Astrophysics Data System (ADS)

Charley, W.; Moran, B.; LaRosa, J.

2012-12-01

In May of 2010, between 14 and 19 inches of rain fell on the Nashville metro area in two days, quickly overwhelming tributaries to the Cumberland River and causing wide-spread, serious flooding. Tractor-trailers and houses were seen floating down Mill Creek, a primary tributary in the south eastern area of Nashville. Twenty-six people died and over 2 billion dollars in damage occurred as a result of the flood. Since that time, several other significant rainfall events have occurred in the area. Emergency responders were unable to deliver aid or preventive measures to areas under threat of flooding (or under water) in time to reduce damages because they could not identify those areas far enough in advance of the floods. Nashville Metro Water, the National Weather Service, the US Geological Survey and the US Army Corps of Engineers established a joint venture to seek ways to better forecast short-term flood events in the region. One component of this effort was a pilot project to compute and display real time inundation maps for Mill Creek, a 108 square-mile basin to the south east of Nashville. HEC-RTS (Real-Time Simulation) was used to assimilate and integrate the hydrologic model HEC-HMS with the hydraulics model HEC-RAS and the inundation mapping program HEC-RAS Mapper. The USGS, along with the other agencies, installed additional precipitation and flow/stage gages in the area. Measurements are recorded every 5-30 minutes and are posted on the USGS NWIS database, which are downloaded by HEC-RTS. Using this data in combination with QPFs (Quantitative Precipitation Forecasts) from the NWS, HEC-RTS applies HEC-HMS and HEC-RAS to estimate current and forecast stage hydrographs. The peak stages are read by HEC-RAS Mapper to compute inundation depths for 6 by 6 foot grid cells. HEC-RTS displays the inundation on a high resolution MrSid aerial photo, along with subbasin boundary, street and various other layers. When a user zooms in and "mouses" over a cell, the inundation depth for that cell is displayed as a tool-tip. This procedure for real-time inundation mapping provides a relatively accurate depiction of water depths throughout the basin, as it is computed using the temporal and spatial distribution of rainfall that has actually occurred and will compute depths based on forecasted rainfall. In addition, the HEC-RAS hydraulics model can be modified as the event is occurring to represent changes in the stream channels, such as obstructions at bridges. This paper covers the procedure used and provides results and images from the integrated models for various precipitation scenarios.

Carbohydrate-protein interactions investigated on plastic chips statically coated with hydrophobically modified hydroxyethylcellulose.

PubMed

Dang, Fuquan; Maeda, Eiki; Osafune, Tomo; Nakajima, Kazuki; Kakehi, Kazuaki; Ishikawa, Mitsuru; Baba, Yoshinobu

2009-12-15

We developed a novel method for rapid screening of carbohydrate-protein interactions using poly(methyl methacrylate) (PMMA) channels statically coated with hydrophobically modified hydroxyethylcellulose (HM-HEC). We found that a self-assembled monolayer (SAM) of HM-HEC on a PMMA surface intact by water allows rapid and reproducible separations of glycan samples using a 20 mM phosphate without HM-HEC. The underlying mechanism for dynamic and static coatings on the PMMA surface is discussed. Simultaneous analysis of the molecular interaction between a complex mixture of carbohydrates from alpha1-acid glycoprotein and proteins has been successfully achieved in PMMA channels statically coated with a SAM of HM-HEC.

Development and characterization of a gastroretentive dosage form composed of chitosan and hydroxyethyl cellulose for alendronate

PubMed Central

Chen, Ying-Chen; Ho, Hsiu-O; Chiu, Chiao-Chi; Sheu, Ming-Thau

2014-01-01

In this study, alendronate, the most commonly used biphosphonate for treating osteoporosis, was formulated as gastroretentive dosage form (GRDF) tablets to enhance its oral bioavailability. GRDF tablets were characterized with the effects of different molecular weights (MWs) of chitosan (CS) and hydroxyethyl cellulose (HEC) at various ratios on swelling, floating, and physical integrity. The CS component was formed using various acids: acetic, lactic, malic, succinic, and citric, and a high viscosity grade of HEC was selected. The results demonstrated that the swelling ratios of the formulations comprising high MW CS were lower than those of low or medium MW CS when salts were formed with any countering acids except for acetic acid. The decreasing ranking of the swelling rates was: CS-citrate > CS-malate > CS-lactate > CS-succinate > CS-acetate. A negative correlation was found between the pKa of the respective countering acid and the swelling rate. The swelling rate was promoted if an acidic salt of CS with a lower water content was incorporated, while it became slower when tablet hardness was higher or the compression force to form tablets was increased. Although HEC did not contribute to swelling or floating, it played a role in maintaining structural integrity. A prolonged dissolution profile of alendronate GRDF tablets developed in this study was observed. PMID:24403821

Aerodynamic Comparison of Hyper-Elliptic Cambered Span (HECS) Wings with Conventional Configurations

NASA Technical Reports Server (NTRS)

Lazos, Barry S.; Visser, Kenneth D.

2006-01-01

An experimental study was conducted to examine the aerodynamic and flow field characteristics of hyper-elliptic cambered span (HECS) wings and compare results with more conventional configurations used for induced drag reduction. Previous preliminary studies, indicating improved L/D characteristics when compared to an elliptical planform prompted this more detailed experimental investigation. Balance data were acquired on a series of swept and un-swept HECS wings, a baseline elliptic planform, two winglet designs and a raked tip configuration. Seven-hole probe wake surveys were also conducted downstream of a number of the configurations. Wind tunnel results indicated aerodynamic performance levels of all but one of the HECS wings exceeded that of the other configurations. The flow field data surveys indicate the HECS configurations displaced the tip vortex farther outboard of the wing than the Baseline configuration. Minimum drag was observed on the raked tip configuration and it was noted that the winglet wake lacked the cohesive vortex structure present in the wakes of the other configurations.

Ririe Dam Release Test Assessment

DTIC Science & Technology

2013-06-01

Notes HEC - RAS Location Station (ft) Observation Notes 1420 Ririe Dam Ririe Dam 119,880 Gates opened and initial release started. 1455 115th St...16°F air temperature. Table A2. Observations made on 11 February 2013. Time Location Notes HEC - RAS Location Station (ft) Observation Notes...ERDC/CRREL TR-13-10 52 Time Location Notes HEC - RAS Location Station (ft) Observation Notes Travel Time* (sec) Vel.** (fps) 1224 5th

Comparison of an extended-release formulation of granisetron (APF530) versus palonosetron for the prevention of chemotherapy-induced nausea and vomiting associated with moderately or highly emetogenic chemotherapy: results of a prospective, randomized, double-blind, noninferiority phase 3 trial.

PubMed

Raftopoulos, Harry; Cooper, William; O'Boyle, Erin; Gabrail, Nashat; Boccia, Ralph; Gralla, Richard J

2015-03-01

Subcutaneous APF530 provides controlled sustained release of granisetron to prevent acute (0-24 h) and delayed (24-120 h) chemotherapy-induced nausea and vomiting (CINV). This randomized, double-blind phase 3 trial compared APF530 and palonosetron in preventing acute and delayed CINV after moderately (MEC) or highly emetogenic chemotherapy (HEC). Patients receiving single-day MEC or HEC received single-dose APF530 250 or 500 mg subcutaneously (SC) (granisetron 5 or 10 mg) or intravenous palonosetron 0.25 mg. Primary objectives were to establish APF530 noninferiority to palonosetron for preventing acute CINV following MEC or HEC and delayed CINV following MEC and to determine APF530 superiority to palonosetron for preventing delayed CINV following HEC. The primary efficacy end point was complete response (CR [using CI difference for APF530-palonosetron]). A lower confidence bound greater than -15 % indicated noninferiority. In the modified intent-to-treat population (MEC = 634; HEC = 707), both APF530 doses were noninferior to palonosetron in preventing acute CINV after MEC (CRs 74.8 % [-9.8, 9.3] and 76.9 % [-7.5, 11.4], respectively, vs. 75.0 % palonosetron) and after HEC (CRs 77.7 % [-11.5, 5.5] and 81.3 % [-7.7, 8.7], respectively, vs. 80.7 % palonosetron). APF530 500 mg was noninferior to palonosetron in preventing delayed CINV after MEC (CR 58.5 % [-9.5, 12.1] vs. 57.2 % palonosetron) but not superior in preventing delayed CINV after HEC. Adverse events were generally mild and unrelated to treatment, the most common (excluding injection-site reactions) being constipation. A single subcutaneous APF530 injection offers a convenient alternative to palonosetron for preventing acute and delayed CINV after MEC or HEC.

Missouri River Recovery Management Plan and Environmental Impact Statement

DTIC Science & Technology

2014-04-11

Proficient in hydrologic and hydraulic engineering computer models, particularly ResSim and HEC - RAS ; working experience with large river systems including...to help study teams determine ecosystem responses to changes in the flow regime of a river or connected wetland. HEC -EFM analyses involve: 1...Description of the Model and How It Will Be Applied in the Study Approval Status HEC - RAS The function of this model is to conduct one-dimensional hydraulic

Regional scale flood modeling using NEXRAD rainfall, GIS, and HEC-HMS/RAS: a case study for the San Antonio River Basin Summer 2002 storm event.

PubMed

Knebl, M R; Yang, Z-L; Hutchison, K; Maidment, D R

2005-06-01

This paper develops a framework for regional scale flood modeling that integrates NEXRAD Level III rainfall, GIS, and a hydrological model (HEC-HMS/RAS). The San Antonio River Basin (about 4000 square miles, 10,000 km2) in Central Texas, USA, is the domain of the study because it is a region subject to frequent occurrences of severe flash flooding. A major flood in the summer of 2002 is chosen as a case to examine the modeling framework. The model consists of a rainfall-runoff model (HEC-HMS) that converts precipitation excess to overland flow and channel runoff, as well as a hydraulic model (HEC-RAS) that models unsteady state flow through the river channel network based on the HEC-HMS-derived hydrographs. HEC-HMS is run on a 4 x 4 km grid in the domain, a resolution consistent with the resolution of NEXRAD rainfall taken from the local river authority. Watershed parameters are calibrated manually to produce a good simulation of discharge at 12 subbasins. With the calibrated discharge, HEC-RAS is capable of producing floodplain polygons that are comparable to the satellite imagery. The modeling framework presented in this study incorporates a portion of the recently developed GIS tool named Map to Map that has been created on a local scale and extends it to a regional scale. The results of this research will benefit future modeling efforts by providing a tool for hydrological forecasts of flooding on a regional scale. While designed for the San Antonio River Basin, this regional scale model may be used as a prototype for model applications in other areas of the country.

Granisetron Extended-Release Injection: A Review in Chemotherapy-Induced Nausea and Vomiting.

PubMed

Deeks, Emma D

2016-12-01

An extended-release (ER) subcutaneously injectable formulation of the first-generation 5-HT 3 receptor antagonist granisetron is now available in the USA (Sustol ® ), where it is indicated for the prevention of acute and delayed chemotherapy-induced nausea and vomiting (CINV) following moderately emetogenic chemotherapy (MEC) or anthracycline and cyclophosphamide combination chemotherapy regimens in adults. Granisetron ER is administered as a single subcutaneous injection and uses an erosion-controlled drug-delivery system to allow prolonged granisetron release. Primary endpoint data from phase III studies after an initial cycle of chemotherapy indicate that, when used as part of an antiemetic regimen, granisetron ER injection is more effective than intravenous ondansetron in preventing delayed CINV following highly emetogenic chemotherapy (HEC); is noninferior to intravenous palonosetron in preventing both acute CINV following MEC or HEC and delayed CINV following MEC; and is similar, but not superior, to palonosetron in preventing delayed CINV following HEC. The benefits of granisetron ER were seen in various patient subgroups, including those receiving anthracycline plus cyclophosphamide-based HEC, and (in an extension of one of the studies) over multiple MEC or HEC cycles. Granisetron ER injection is generally well tolerated, with an adverse event profile similar to that of ondansetron or palonosetron. Thus, granisetron ER injection expands the options for preventing both acute and delayed CINV in adults with cancer receiving MEC or anthracycline plus cyclophosphamide-based HEC.

Thiolated hydroxyethylcellulose: synthesis and in vitro evaluation.

PubMed

Sarti, Federica; Staaf, Alexander; Sakloetsakun, Duangkamon; Bernkop-Schnürch, Andreas

2010-11-01

In recent years, thiomers have received considerable interest due to advantageous characteristics, such as improved mucoadhesive and permeation enhancing properties. Thiolated polymers, however, are characterized by an ionic charge which represents for various applications a great limitation. The aim of this study was therefore to synthesize a novel thiolated polymer not exhibiting ionizable groups. Hydroxyethylcellulose (HEC) was chosen as polymer backbone. The chemical modification was achieved by the replacement of hydroxyl groups on the carbohydrate structure with thiol moieties, using thiourea as thiolating reagent. The resulting thiolated hydroxyethylcellulose (HEC-SH) was characterized in vitro regarding its gelling properties, swelling behaviour, mucoadhesion on freshly excised porcine intestinal mucosa and permeation enhancing effect across rat intestinal mucosa. The new thiomer displayed up to 131.58 ± 11.17 μmol thiol groups per gram polymer, which are responsible for the observed in situ gelling capacity. The swelling behaviour and the mucoadhesive properties of tablets based on HEC-SH were 1.5-fold and 4-fold improved compared with unmodified HEC, respectively. The permeation enhancing effect of 0.5% (m/v) HEC-SH on rhodamine 123 (Rho-123) transport was 1.9-fold improved compared with buffer only. According to these results, HEC-SH seems to represent a promising tool for the development of in situ gelling, mucoadhesive delivery systems with permeation enhancing properties. Copyright © 2010 Elsevier B.V. All rights reserved.

Osteopontin Promotes Invasion, Migration and Epithelial-Mesenchymal Transition of Human Endometrial Carcinoma Cell HEC-1A Through AKT and ERK1/2 Signaling.

PubMed

Li, Yinghua; Xie, Yunpeng; Cui, Dan; Ma, Yanni; Sui, Linlin; Zhu, Chenyang; Kong, Hui; Kong, Ying

2015-01-01

Osteopontin (OPN) is an Extracellular Matrix (ECM) molecule and is involved in many physiologic and pathologic processes, including cell adhesion, angiogenesis and tumor metastasis. OPN is a well-known multifunctional factor involved in various aspects of cancer progression, including endometrial cancer. In this study, we examined the significance of OPN in endometrial cancer. The proliferation, migration and invasion ability of HEC-1A cells were detected by Cell Counting Kit-8 (CCK-8), Wound scratch assay and transwell. Western blots were employed to detect the expression of Matrix metalloproteinase-2 (MMP-2) and epithelial-mesenchymal transition (EMT)-related factors in HEC-1A cells treated with rhOPN. rhOPN promotes cell proliferation, migration and invasion in HEC-1A cells. rhOPN influenced EMT-related factors and MMP-2 expression in HEC-1A cells. rhOPN promoted HEC-1A cells migration, invasion and EMT through protein kinase B (PKB/AKT) and Extracellular regulated protein kinases (ERK1/2) signaling pathway. These results may open up a novel therapeutic strategy for endometrial cancer: namely, rhOPN have important roles in controlling growth of endometrial of cancer cells and suggest a novel target pathway for treatment of this cancer. © 2015 The Author(s) Published by S. Karger AG, Basel.

Preparation and characterization of poly(acrylic acid)-hydroxyethyl cellulose graft copolymer.

PubMed

Abdel-Halim, E S

2012-10-01

Poly(acrylic acid) hydroxyethyl cellulose [poly(AA)-HEC] graft copolymer was prepared by polymerizing acrylic acid (AA) with hydroxyethyl cellulose (HEC) using potassium bromate/thiourea dioxide (KBrO(3)/TUD) as redox initiation system. The polymerization reaction was carried out under a variety of conditions including concentrations of AA, KBrO(3) and TUD, material to liquor ratio and polymerization temperature. The polymerization reaction was monitored by withdrawing samples from the reaction medium and measuring the total conversion. The rheological properties of the poly(AA)-HEC graft copolymer were investigated. The total conversion and rheological properties of the graft copolymer depended on the ratio of KBrO(3) to TUD and on acrylic acid concentration as well as temperature and material to liquor ratio. Optimum conditions of the graft copolymer preparation were 30 mmol KBrO(3) and 30 mmol TUD/100g HEC, 100% AA (based on weight of HEC), duration 2h at temperature 50 °C using a material to liquor ratio of 1:10. Copyright © 2012. Published by Elsevier Ltd.

Graphene-oxide stabilization in electrolyte solutions using hydroxyethyl cellulose for drug delivery application.

PubMed

Mianehrow, Hanieh; Moghadam, Mohamad Hasan Mohamadzadeh; Sharif, Farhad; Mazinani, Saeedeh

2015-04-30

Stabilization of graphene oxide (GO) in physiological solution is performed using hydroxyethyl cellulose (HEC) to make the resultant nanohybrid suitable for targeted drug delivery purposes. Short and long term stability of GO suspensions with different ionic strengths were assessed using ultraviolet-visible spectroscopy (UV-vis), atomic force microscopy (AFM) and zeta potential measurements. Results depicted that HEC effectively stabilized GO in electrolyte solutions and the mechanism of stabilization appeares to be depended on HEC content. Drug loading and release behavior of folic acid (FA) as a model drug, from GO-HEC nanohybrid were studied to assess its application in drug delivery systems. Results showed the nanohybrid could be highly loaded by folic acid. Moreover, HEC content in the nanohybrid played an important role in final application to make it applicable either as a carrier for controllable drug release or as a folate-targeted drug carrier. In addition, according to cytotoxicity results, the nanohybrid showed good biocompatibility which indeed confirms its potential application as a drug carrier. Copyright © 2015 Elsevier B.V. All rights reserved.

Discovery of 4-aryl-N-arylcarbonyl-2-aminothiazoles as Hec1/Nek2 inhibitors. Part I: optimization of in vitro potencies and pharmacokinetic properties.

PubMed

Lee, Ying-Shuan E; Chuang, Shih-Hsien; Huang, Lynn Y L; Lai, Chun-Liang; Lin, Yu-Hsiang; Yang, Ju-Ying; Liu, Chia-Wei; Yang, Sheng-chuan; Lin, Her-Sheng; Chang, Chia-chi; Lai, Jun-Yu; Jian, Pei-Shiou; Lam, King; Chang, Jia-Ming; Lau, Johnson Y N; Huang, Jiann-Jyh

2014-05-22

A series of 4-aryl-N-arylcarbonyl-2-aminothiazoles of scaffold 4 was designed and synthesized as Hec1/Nek2 inhibitors. Structural optimization of 4 led to compound 32 bearing C-4' 4-methoxyphenoxy and 4-(o-fluoropyridyl)carbonyl groups that showed low nanomolar in vitro antiproliferative activity (IC50: 16.3-42.7 nM), high intravenous AUC (64.9 μM·h, 2.0 mg/kg) in SD rats, and significant in vivo antitumor activity (T/C = 32%, 20 mg/kg, IV) in mice bearing human MDA-MB-231 xenografts. Cell responses resulting from Hec1/Nek2 inhibition were observed in cells treated with 32, including a reduced level of Hec1 coimmunoprecipitated with Nek2, degradation of Nek2, mitotic abnormalities, and apoptosis. Compound 32 showed selectivity toward cancer cells over normal phenotype cells and was inactive in a [(3)H]astemizole competitive binding assay for hERG liability screening. Therefore, 32 is as a good lead toward the discovery of a preclinical candidate targeting Hec1/Nek2 interaction.

Reasoning Backwards by Design: Commentary on "Moral Reasoning among HEC Members".

PubMed

Stephens, Ashley L; Heitman, Elizabeth

2015-01-01

Empirical assessment of the practice of clinical ethics is made difficult by the limited standardization of settings, structures, processes, roles, and training for ethics consultation, as well as by whether individual ethics consultants or hospital ethics committees (HECs) provide consultation. Efforts to study the relationship between theory and practice in the work of HECs likewise require the spelling out of assumptions and definition of key variables, based in knowledge of the core concepts of clinical ethics and logistics of clinical consultation. The survey of HEC members reported by Wasserman and colleagues illustrates the difficulty of such research and calls attention to need for studies of real-time, complex decision making to inform conclusions about how theory affects practice. Copyright 2015 The Journal of Clinical Ethics. All rights reserved.

High-End Computing Challenges in Aerospace Design and Engineering

NASA Technical Reports Server (NTRS)

Bailey, F. Ronald

2004-01-01

High-End Computing (HEC) has had significant impact on aerospace design and engineering and is poised to make even more in the future. In this paper we describe four aerospace design and engineering challenges: Digital Flight, Launch Simulation, Rocket Fuel System and Digital Astronaut. The paper discusses modeling capabilities needed for each challenge and presents projections of future near and far-term HEC computing requirements. NASA's HEC Project Columbia is described and programming strategies presented that are necessary to achieve high real performance.

A Self-Powered Thin-Film Radiation Detector Using Intrinsic High-Energy Current (HEC) (Author’s Final Version)

DTIC Science & Technology

2016-09-08

10.1118/1.4935531. A new radiation detection method relies on high-energy current (HEC) formed by secondary charged particles in the detector material...photocurrent, radiation detection , self-powered, thin-film U U U SAR 17 Dr. Joseph Wander Reset A Self-powered thin-film radiation detector using intrinsic...Program, Lowell, MA 01854 Purpose: We introduce a radiation detection method that relies on high-energy current (HEC) formed by secondary 10 charged

Loss of Life, Evacuation and Emergency Management: Comparison and Application to Case Studies in the USA

DTIC Science & Technology

2013-01-22

eK ay a nd M cC le lla nd G ra ha m Katrina HEC -FIA LifeSim Figure 1: Comparison of loss of life models (based on Johnstone et al., 2005...Katrina HEC FIA Lifesim Application: flood types Levee breaching, river , coastal Levee breaching, river , coastal levee breaching, dam failure...Mortality – Overtopping with breach Center Side of American River (OTSC) Figure 31: Mortality for the HEC -FIA method for the two

AutoRoute Rapid Flood Inundation Model

DTIC Science & Technology

2013-03-01

Res. 33(2): 309-319. U.S. Army Engineer Hydrologic Engineering Center. 2010. “ HEC - RAS : River Analysis System, User’s Manual, Version 4.1.” Davis...cross-section data does not exist. As such, the AutoRoute model is not meant to be as accurate as models such as HEC - RAS (U.S. Army Engineer...such as HEC - RAS assume that the defined low point of cross sections must be connected. However, in this approach the channel is assumed to be defined

Lysophosphatidic acid (LPA) effects on endometrial carcinoma in vitro proliferation, invasion, and matrix metalloproteinase activity.

PubMed

Wang, Feng-qiang; Ariztia, Edgardo V; Boyd, Leslie R; Horton, Faith R; Smicun, Yoel; Hetherington, Jessica A; Smith, Phillip J; Fishman, David A

2010-04-01

Lysophosphatidic acid (LPA) has potent growth-regulatory effect in many cell types and has been linked to the in vivo tumor growth and metastasis in several malignancies. The goal of this study was to assess the regulation of (EC) microenvironment by LPA through the examination of its effect on cell proliferation, migration, invasion, uPA activity, and matrix metalloproteinase (MMP) secretion/activation. All experiments were performed in vitro using an EC cell line, HEC-1A. Cell proliferation was determined using the Promega MTS proliferation assay following 48 h of exposures to different concentrations of LPA (0.1, 1.0 and 10.0 microM). Cell invasion was assessed using a modified Boyden chamber assay with collagen I coated on the membrane. HEC-1A motility was examined by Boyden chamber migration assay as well as the scratch wound closure assay on type I collagen. MMP secretion/activation in HEC-1A conditioned medium was detected by gelatin zymography. MMP-7 mRNA expression was determined using real-time PCR. uPA activity was measured using a coupled colorimetric assay. LPA, at the concentrations of 0.1 and 1.0 microM, significantly induced the proliferation of HEC-1A cells (p<0.01). At 10 microM, LPA- induced HEC-1A proliferation to a less extent and showed no significant effect on HEC-1A invasion and migration (p>0.05). Gelatin zymogram showed that HEC-1A cells secreted high levels of MMP-7, while MMP-2 and MMP-9 are barely detectable. In addition, LPA significantly enhanced uPA activity in HEC-1A conditioned medium in a concentration-dependent manner. LPA is a potent modulator of cellular proliferation and invasion for EC cells. It also has the capacity to stimulate the secretion/activity of uPA and MMP-7. Those results suggest that LPA is a bioactive modulator of EC microenvironment and may have a distinct regulation mechanism as observed in epithelial ovarian cancer. Copyright 2009. Published by Elsevier Inc.

Growth of MCF-7 breast cancer cells and efficacy of anti-angiogenic agents in a hydroxyethyl chitosan/glycidyl methacrylate hydrogel.

PubMed

Wang, Hejing; Qian, Junmin; Zhang, Yaping; Xu, Weijun; Xiao, Juxiang; Suo, Aili

2017-01-01

Breast cancer negatively affects women's health worldwide. The tumour microenvironment plays a critical role in tumour initiation, proliferation, and metastasis. Cancer cells are traditionally grown in two-dimensional (2D) cultures as monolayers on a flat solid surface lacking cell-cell and cell-matrix interactions. These experimental conditions deviate from the clinical situation. Improved experimental systems that can mimic the in vivo situation are required to discover new therapies, particularly for anti-angiogenic agents that mainly target intercellular factors and play an essential role in treating some cancers. Chitosan can be modified to construct three-dimensional (3D) tumour models. Here, we report an in vitro 3D tumour model using a hydroxyethyl chitosan/glycidyl methacrylate (HECS-GMA) hydrogel produced by a series of chitosan modifications. Parameters relating to cell morphology, viability, proliferation, and migration were analysed using breast cancer MCF-7 cells. In a xenograft model, secretion of angiogenesis-related growth factors and the anti-angiogenic efficacy of Endostar and Bevacizumab in cells grown in HECS-GMA hydrogels were assessed by immunohistochemistry. Hydroxyethyl chitosan/glycidyl methacrylate hydrogels had a highly porous microstructure, mechanical properties, swelling ratio, and morphology consistent with a 3D tumour model. Compared with a 2D monolayer culture, breast cancer MCF-7 cells residing in the HECS-GMA hydrogels grew as tumour-like clusters in a 3D formation. In a xenograft model, MCF-7 cells cultured in the HECS-GMA hydrogels had increased secretion of angiogenesis-related growth factors. Recombinant human endostatin (Endostar), but not Bevacizumab (Avastin), was an effective anti-angiogenic agent in HECS-GMA hydrogels. The HECS-GMA hydrogel provided a 3D tumour model that mimicked the in vivo cancer microenvironment and supported the growth of MCF7 cells better than traditional tissue culture plates. The HECS-GMA hydrogel may offer an improved platform to minimize the gap between traditional tissue culture plates and clinical applicability. In addition, the anti-angiogenic efficacy of drugs such as Endostar and Bevacizumab can be more comprehensively studied and assessed in HECS-GMA hydrogels.

Future efficiency of run of the river hydropower schemes based on climate change scenarios: case study in UK catchments

NASA Astrophysics Data System (ADS)

Pasten Zapata, Ernesto; Moggridge, Helen; Jones, Julie; Widmann, Martin

2017-04-01

Run-of-the-River (ROR) hydropower schemes are expected to be importantly affected by climate change as they rely in the availability of river flow to generate energy. As temperature and precipitation are expected to vary in the future, the hydrological cycle will also undergo changes. Therefore, climate models based on complex physical atmospheric interactions have been developed to simulate future climate scenarios considering the atmosphere's greenhouse gas concentrations. These scenarios are classified according to the Representative Concentration Pathways (RCP) that are generated according to the concentration of greenhouse gases. This study evaluates possible scenarios for selected ROR hydropower schemes within the UK, considering three different RCPs: 2.6, 4.5 and 8.5 W/m2 for 2100 relative to pre-industrial values. The study sites cover different climate, land cover, topographic and hydropower scheme characteristics representative of the UK's heterogeneity. Precipitation and temperature outputs from state-of-the-art Regional Climate Models (RCMs) from the Euro-CORDEX project are used as input for a HEC-HMS hydrological model to simulate the future river flow available. Both uncorrected and bias-corrected RCM simulations are analyzed. The results of this project provide an insight of the possible effects of climate change towards the generation of power from the ROR hydropower schemes according to the different RCP scenarios and contrasts the results obtained from uncorrected and bias-corrected RCMs. This analysis can aid on the adaptation to climate change as well as the planning of future ROR schemes in the region.

Comparison of 2D numerical models for river flood hazard assessment: simulation of the Secchia River flood in January, 2014

NASA Astrophysics Data System (ADS)

Shustikova, Iuliia; Domeneghetti, Alessio; Neal, Jeffrey; Bates, Paul; Castellarin, Attilio

2017-04-01

Hydrodynamic modeling of inundation events still brings a large array of uncertainties. This effect is especially evident in the models run for geographically large areas. Recent studies suggest using fully two-dimensional (2D) models with high resolution in order to avoid uncertainties and limitations coming from the incorrect interpretation of flood dynamics and an unrealistic reproduction of the terrain topography. This, however, affects the computational efficiency increasing the running time and hardware demands. Concerning this point, our study evaluates and compares numerical models of different complexity by testing them on a flood event that occurred in the basin of the Secchia River, Northern Italy, on 19th January, 2014. The event was characterized by a levee breach and consequent flooding of over 75 km2 of the plain behind the dike within 48 hours causing population displacement, one death and economic losses in excess of 400 million Euro. We test the well-established TELEMAC 2D, and LISFLOOD-FP codes, together with the recently launched HEC-RAS 5.0.3 (2D model), all models are implemented using different grid size (2-200 m) based on the 1 m digital elevation model resolution. TELEMAC is a fully 2D hydrodynamic model which is based on the finite-element or finite-volume approach. Whereas HEC-RAS 5.0.3 and LISFLOOD-FP are both coupled 1D-2D models. All models are calibrated against observed inundation extent and maximum water depths, which are retrieved from remotely sensed data and field survey reports. Our study quantitatively compares the three modeling strategies highlighting differences in terms of the ease of implementation, accuracy of representation of hydraulic processes within floodplains and computational efficiency. Additionally, we look into the different grid resolutions in terms of the results accuracy and computation time. Our study is a preliminary assessment that focuses on smaller areas in order to identify potential modeling schemes that would be efficient for simulating flooding scenarios for large and very large floodplains. This research aims at contributing to the reduction of uncertainties and limitations in hazard and risk assessment.

Cost-utility and budget impact analyses of the use of NEPA for chemotherapy-induced nausea and vomiting prophylaxis in Italy

PubMed Central

Restelli, Umberto; Saibene, Gabriella; Nardulli, Patrizia; Di Turi, Roberta; Bonizzoni, Erminio; Scolari, Francesca; Perrone, Tania; Croce, Davide; Celio, Luigi

2017-01-01

Objective To evaluate the efficiency of resources allocation and sustainability of the use of netupitant+palonosetron (NEPA) for chemotherapy-induced nausea and vomiting (CINV) prophylaxis assuming the Italian National Health Service (NHS) perspective. A published Markov model was adapted to assess the incremental cost-utility ratio of NEPA compared with aprepitant (APR) + palonosetron (PALO), fosaprepitant (fAPR) + PALO, APR + ondansetron (ONDA), fAPR + ONDA in patients receiving a highly emetogenic chemotherapy (HEC) and with APR + PALO and fAPR + PALO in patients receiving a moderately emetogenic chemotherapy (MEC). Setting Oncology hospital department in Italy. Methods A Markov model was used to determine the impact of NEPA on the budget of the Italian NHS on a 5-day time horizon, corresponding to the acute and delayed CINV prophylaxis phases. Direct medical costs considered were related to antiemetic drugs, adverse events management, CINV episodes management. Clinical and quality of life data referred to previously published works. The budget impact analysis considered the aforementioned therapies plus PALO alone (for HEC and MEC) on a 5-year time horizon, comparing two scenarios: one considering the use of NEPA and one not considering its use. Primary and secondary outcome measures Incremental cost per quality adjusted life year (QALY) and differential economic impact for the Italian NHS between the two scenarios considered. Results NEPA is more effective and less expensive (dominant) compared with APR + PALO (for HEC and MEC), fAPR + PALO (for HEC and MEC), APR + ONDA (for HEC), fAPR + ONDA (for HEC). The use of NEPA would lead to a 5-year cost decrease of €63.7 million (€42.7 million for HEC and €20.9 million for MEC). Conclusions NEPA allows an efficient allocation of resources for the Italian NHS and it is sustainable, leading to a cost decrease compared with a scenario which does not consider its use. PMID:28765126

The impact of 5-hydroxytryptamine-receptor antagonists on chemotherapy treatment adherence, treatment delay, and nausea and vomiting.

PubMed

Palli, Swetha Rao; Grabner, Michael; Quimbo, Ralph A; Rugo, Hope S

2015-01-01

To determine the incidence of chemotherapy-induced nausea/vomiting (CINV) and chemotherapy treatment delay and adherence among patients receiving palonosetron versus other 5-hydroxytryptamine receptor antagonist (5-HT3 RA) antiemetics. This retrospective claims analysis included adults with primary malignancies who initiated treatment consisting of single-day intravenous highly emetogenic chemotherapy (HEC) or moderately EC (MEC) regimens. Treatment delay was defined as a gap in treatment at least twice the National Comprehensive Cancer Network-specified cycle length, specific to each chemotherapy regimen. Treatment adherence was determined by the percentage of patients who received the regimen-specific recommended number of chemotherapy cycles within the recommended time frame. We identified 1,832 palonosetron and 2,387 other 5-HT3 RA ("other") patients who initiated HEC therapy, and 1,350 palonosetron users and 1,379 patients on other antiemetics who initiated MEC therapy. Fewer patients receiving palonosetron experienced CINV versus other (HEC, 27.5% versus 32.2%, P=0.0011; MEC, 36.1% versus 41.7%, P=0.0026), and fewer treatment delays occurred among patients receiving palonosetron versus other (HEC, 3.2% versus 6.0%, P<0.0001; MEC, 17.0% versus 26.8%, P<0.0001). Compared with the other cohort, patients receiving palonosetron were significantly more adherent to the index chemotherapy regimen with respect to the recommended time frame (HEC, 74.7% versus 69.7%, P=0.0004; MEC, 43.1% versus 37.3%, P=0.0019) and dosage (HEC, 27.3% versus 25.8%, P=0.0004; MEC, 15.0% versus 12.6%, P=0.0019). Palonosetron more effectively reduced occurrence of CINV in patients receiving HEC or MEC compared with other agents in this real-world setting. Additionally, patients receiving palonosetron had better adherence and fewer treatment delays than patients receiving other 5-HT3 RAs.

The impact of 5-hydroxytryptamine-receptor antagonists on chemotherapy treatment adherence, treatment delay, and nausea and vomiting

PubMed Central

Palli, Swetha Rao; Grabner, Michael; Quimbo, Ralph A; Rugo, Hope S

2015-01-01

Purpose To determine the incidence of chemotherapy-induced nausea/vomiting (CINV) and chemotherapy treatment delay and adherence among patients receiving palonosetron versus other 5-hydroxytryptamine receptor antagonist (5-HT3 RA) antiemetics. Materials and methods This retrospective claims analysis included adults with primary malignancies who initiated treatment consisting of single-day intravenous highly emetogenic chemotherapy (HEC) or moderately EC (MEC) regimens. Treatment delay was defined as a gap in treatment at least twice the National Comprehensive Cancer Network-specified cycle length, specific to each chemotherapy regimen. Treatment adherence was determined by the percentage of patients who received the regimen-specific recommended number of chemotherapy cycles within the recommended time frame. Results We identified 1,832 palonosetron and 2,387 other 5-HT3 RA (“other”) patients who initiated HEC therapy, and 1,350 palonosetron users and 1,379 patients on other antiemetics who initiated MEC therapy. Fewer patients receiving palonosetron experienced CINV versus other (HEC, 27.5% versus 32.2%, P=0.0011; MEC, 36.1% versus 41.7%, P=0.0026), and fewer treatment delays occurred among patients receiving palonosetron versus other (HEC, 3.2% versus 6.0%, P<0.0001; MEC, 17.0% versus 26.8%, P<0.0001). Compared with the other cohort, patients receiving palonosetron were significantly more adherent to the index chemotherapy regimen with respect to the recommended time frame (HEC, 74.7% versus 69.7%, P=0.0004; MEC, 43.1% versus 37.3%, P=0.0019) and dosage (HEC, 27.3% versus 25.8%, P=0.0004; MEC, 15.0% versus 12.6%, P=0.0019). Conclusion Palonosetron more effectively reduced occurrence of CINV in patients receiving HEC or MEC compared with other agents in this real-world setting. Additionally, patients receiving palonosetron had better adherence and fewer treatment delays than patients receiving other 5-HT3 RAs. PMID:26124681

A model study of bridge hydraulics

DOT National Transportation Integrated Search

2010-08-01

Most flood studies in the United States use the Army Corps of Engineers HEC-RAS (Hydrologic Engineering : Centers River Analysis System) computer program. This study was carried out to compare results of HEC-RAS : bridge modeling with laboratory e...

Slow-release granisetron (APF530) versus palonosetron for chemotherapy-induced nausea/vomiting: analysis by American Society of Clinical Oncology emetogenicity criteria.

PubMed

Raftopoulos, Harry; Boccia, Ralph; Cooper, William; O'Boyle, Erin; Gralla, Richard J

2015-09-01

APF530 is a novel sustained-release formulation of granisetron. In a Phase III trial, APF530 500 mg was noninferior to palonosetron 0.25 mg in preventing acute chemotherapy-induced nausea and vomiting (CINV) after moderately (MEC) or highly emetogenic chemotherapy (HEC) and delayed CINV after MEC, but not superior in preventing delayed CINV after HEC. Emetogenicity was classified by Hesketh criteria; this reanalysis uses newer American Society of Clinical Oncology criteria. Complete responses (no emesis or rescue medication) after cycle one were reanalyzed after reclassification of MEC and HEC by American Society of Clinical Oncology criteria. APF530 maintained noninferiority to palonosetron. Single-dose APF530 is a promising alternative to palonosetron for preventing acute and delayed CINV after MEC or HEC. The Clinicaltrials.gov identifier for this study is NCT00343460.

Ngas Multi-Stage Coaxial High Efficiency Cooler (hec)

NASA Astrophysics Data System (ADS)

Nguyen, T.; Toma, G.; Jaco, C.; Raab, J.

2010-04-01

This paper presents the performance data of the single and two-stage High Efficiency Cooler (HEC) tested with coaxial cold heads. The single stage coaxial cold head has been optimized to operate at temperatures of 40 K and above. The two-stage parallel cold head configuration has been optimized to operate at 30 K and above and provides a long-life, low mass and efficient two-stage version of the Northrop Grumman Aerospace Systems (NGAS) flight qualified single stage HEC cooler. The HEC pulse tube cryocoolers are the latest generation of flight coolers with heritage to the 12 Northrop Grumman Aerospace Systems (NGAS) coolers currently on orbit with 2 operating for more than 11.5 years. This paper presents the performance data of the one and two-stage versions of this cooler under a wide range of heat rejection temperature, cold head temperature and input power.

Comparison of two plethysmography systems in assessment of forearm blood flow.

PubMed

Leslie, Stephen J; Attinà, Teresa; Hultsch, Ellen; Bolscher, Luc; Grossman, Matthias; Denvir, Martin A; Webb, David J

2004-05-01

Venous occlusion plethysmography is widely used to assess forearm blood flow (FBF). We compared the established Hokanson system (HEC4) with a newly developed Filtrass 2001 system (F2001). The HEC4 uses mercury-in-Silastic strain gauges, whereas F2001 detects volume changes with a nonmercury linear displacement device. The aim of this study was to evaluate the new F2001 against the HEC4 in terms of repeatability and systematic bias. Ten subjects were studied on 4 separate days in random order using either the HEC4 on both arms, the F2001 on both arms, the HEC4 on the right arm with the F2001 on the left, or the F2001 on the right arm and the HEC4 on the left. Stroop's colored word conflict test and postocclusive hyperemia were used to increase FBF, and lower body negative pressure was used to lower FBF. Stroop's colored word conflict test and lower body negative pressure increased (24.6 +/- 1.5%, n = 240, P < 0.0001) and decreased (18.7 +/- 0.8%, n = 240, P < 0.0001) FBF, respectively. Postocclusive hyperemia after occlusion times of 5, 8, and 13 min substantially increased FBF by 390 +/- 86, 756 +/- 217, and 851 +/- 132%, respectively. Repeatability was not different between the devices (0.10 +/- 2.37 vs. -0.47 +/- 1.92 l/min, n = 125, P > 0.05), and there was no systematic bias. The F2001 is a newly developed plethysmography system that does not utilize mercury and is suitable for assessing changes of FBF in physiological studies.

An innovative modeling approach using Qual2K and HEC-RAS integration to assess the impact of tidal effect on River Water quality simulation.

PubMed

Fan, Chihhao; Ko, Chun-Han; Wang, Wei-Shen

2009-04-01

Water quality modeling has been shown to be a useful tool in strategic water quality management. The present study combines the Qual2K model with the HEC-RAS model to assess the water quality of a tidal river in northern Taiwan. The contaminant loadings of biochemical oxygen demand (BOD), ammonia nitrogen (NH(3)-N), total phosphorus (TP), and sediment oxygen demand (SOD) are utilized in the Qual2K simulation. The HEC-RAS model is used to: (i) estimate the hydraulic constants for atmospheric re-aeration constant calculation; and (ii) calculate the water level profile variation to account for concentration changes as a result of tidal effect. The results show that HEC-RAS-assisted Qual2K simulations taking tidal effect into consideration produce water quality indices that, in general, agree with the monitoring data of the river. Comparisons of simulations with different combinations of contaminant loadings demonstrate that BOD is the most import contaminant. Streeter-Phelps simulation (in combination with HEC-RAS) is also performed for comparison, and the results show excellent agreement with the observed data. This paper is the first report of the innovative use of a combination of the HEC-RAS model and the Qual2K model (or Streeter-Phelps equation) to simulate water quality in a tidal river. The combination is shown to provide an alternative for water quality simulation of a tidal river when available dynamic-monitoring data are insufficient to assess the tidal effect of the river.

Chemotherapy-induced nausea and vomiting is less controlled at delayed phase in patients with esophageal cancer: a prospective registration study by the CINV Study Group of Japan.

PubMed

Baba, Yoshifumi; Baba, Hideo; Yamamoto, Sachiko; Shimada, Hideaki; Shibata, Tomotaka; Miyazaki, Tatsuya; Yoshikawa, Takaki; Nakajima, Yasuaki; Tsuji, Yasushi; Shimokawa, Mototsugu; Kitagawa, Yuko; Aiba, Keisuke

2017-02-01

Chemotherapy is an indispensable therapeutic approach for esophageal cancer. Although chemotherapy-induced nausea and vomiting (CINV) is one of the most crucial adverse events, the current state of CINV in patients with esophageal cancer remains unclear. This multicenter prospective observational study analyzed data for 192 patents with esophageal cancer who underwent moderately emetogenic chemotherapy (MEC) or highly emetogenic chemotherapy (HEC). The patients recorded their CINV incidence and severity daily for 7 days after receiving chemotherapy, using visual analog scales (VAS). Of the 192 patients, 181 received HEC including cisplatin, and 11 patients received MEC including nedaplatin. Approximately 81% of HEC and 82% of MEC patients received antiemetic therapy in compliance with guidelines. Although CINV was controlled relatively well in the early phase (days 1-4), it was not fully controlled in late phase (days 5-7) for both the HEC and MEC groups. Female sex was a major risk factor for delayed vomiting (P=0.034). Multivariate logistic regression analysis for VAS revealed that motion sickness, age, and use of other antiemetics were risk factors for delayed nausea. Adherence to antiemetic guidelines effectively controls vomiting but is less effective against delayed CINV in both HEC and MEC patients. Identification of individual risk factors, such as female sex, will help develop personalized treatments for CINV. In the clinical setting for esophageal cancer, regimens that include nedaplatin might need to be treated as HEC. © 2016 International Society for Diseases of the Esophagus.

Nonaqueous lithium-ion capacitors with high energy densities using trigol-reduced graphene oxide nanosheets as cathode-active material.

PubMed

Aravindan, Vanchiappan; Mhamane, Dattakumar; Ling, Wong Chui; Ogale, Satishchandra; Madhavi, Srinivasan

2013-12-01

One HEC of a material: The use of trigol-reduced graphene oxide nanosheets as cathode material in hybrid lithium-ion electrochemical capacitors (Li-HECs) results in an energy density of 45 Wh kg(-1) ; much enhanced when compared to similar devices. The mass loading of the active materials is optimized, and the devices show good cycling performance. Li-HECs employing these materials outperform other supercapacitors, making them attractive for use in power sources. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Loss of Life, Evacuation and Emergency Management - Application of Dutch Models to US Case Studies

DTIC Science & Technology

2012-12-18

Risks to people M es o: Z on e or lo ca tio n Instability tests Jonkman 1953 D eK ay a nd M cC le lla nd G ra ha m Katrina HEC -FIA...mortality functions (Jonkman) o 2.2.2 New Orleans / Katrina mortality functions (Jonkman) o 2.2.3 HEC FIA approach (USACE) o 2.2.4. Loss of life methods...comparison (USACE) • 2.3 Dutch Evacuation and Evacuaid approach (Kolen) o 2.3.1 Evacuation approach implemented in HEC FIA (USACE) o 2.3.2 Evacuation

GIS For Floodplain Mapping in Design of Highway Drainage Facilities

DOT National Transportation Integrated Search

1998-08-01

Since the 1960s,civil engineers have employed a variety of computer models for stream floodplain analysis. HEC-2 and its Windows counterpart, HEC-RAS, have been the principal models used for such analyses. A significant deficiency of these programs i...

Application of HEC-6 to ephemeral rivers of Arizona

DOT National Transportation Integrated Search

1986-01-01

The U.S. Army Corps of Engineers, computer program HEC-6--"Scour and Deposition in Rivers and Reservoirs" was applied to three ephemeral rivers of Arizona--Agua Fria River, Salt River, and Rillito Creek. The input data development techniques and resu...

Development of recombinant adeno-associated virus vectors carrying small interfering RNA (shHec1)-mediated depletion of kinetochore Hec1 protein in tumor cells.

PubMed

Li, L; Yang, L; Scudiero, D A; Miller, S A; Yu, Z-X; Stukenberg, P T; Shoemaker, R H; Kotin, R M

2007-05-01

Transcript depletion using small interfering RNA (siRNA) technology represents a potentially valuable technique for the treatment of cancer. However, delivering therapeutic quantities of siRNA into solid tumors by chemical transfection is not feasible, whereas viral vectors efficiently transduce many human tumor cell lines. Yet producing sufficient quantities of viral vectors that elicit acute and selective cytotoxicity remains a major obstacle for preclinical and clinical trials. Using the invertebrate Spodoptera frugiperda (Sf9) cell line, we were able to produce high titer stocks of cytotoxic recombinant adeno-associated virus (rAAV) that express short hairpin RNA (shRNA) and that efficiently deplete Hec1 (highly expressed in cancer 1), or Kntc2 (kinetochore-associated protein 2), a kinetochore protein directly involved in kinetochore microtubule interactions, chromosome congression and spindle checkpoint signaling. Depletion of Hec1 protein results in persistent spindle checkpoint activation followed by cell death. Because Hec1 expression and activity are only present in mitotic cells, non-dividing cells were not affected by rAAV treatment. On the basis of the results of screening 56 human tumor cell lines with three different serotype vectors, we used a tumor xenograft model to test the effects in vivo. The effects of the shHec1 vector were evident in sectioned and stained tumors. The experiments with rAAV-shRNA vectors demonstrate the utility of producing vectors in invertebrate cells to obtain sufficient concentrations and quantities for solid tumor therapy. This addresses an important requirement for cancer gene therapy, to produce cytotoxic vectors in sufficient quantities and concentrations to enable quantitative transduction and selective killing of solid tumor cells.

Occurrence of putative virulence genes on Arcobacter butzleri isolated from three different environmental sites throughout the dairy chain.

PubMed

Piva, S; Gariano, G R; Bonilauri, P; Giacometti, F; Decastelli, L; Florio, D; Massella, E; Serraino, A

2017-04-01

This comparative study investigated the occurrence of cadF, cj1349, ciaB, pldA, tlyA, hecA, hecB, mviN, irgA and IroE genes in 212 Arcobacter butzleri isolated from three different environmental sites linked to the dairy chain (farms, industrial and artisanal dairy plants) located in three Italian regions (Lombardy, Emilia-Romagna and Calabria). According to the presence of these genes, different pathotypes (P-types) were determined. The main genes detected were ciaB, mviN, tlyA, cj1349, pldA and cadF, while the least common genes were iroE, hecA, hecB and irgA. TlyA, irgA, hecA, hecB and iroE, which were significantly more frequent in isolates recovered in industrial dairy plants. Twelve P-types were detected. The occurrence of the most frequently detected P-types (P-types 1, 2, 3 and 5) differed significantly (P < 0·001) in relation to both the environmental site and geographical area of isolation. The highest diversity in P-types was observed in industrial dairy plants and in the Calabria region. The results of this study show a correlation between the occurrence of putative virulence genes and virulence genotype variability depending on the environmental site and geographical origin of the isolates. The present study provides insights into the similar distribution of putative virulence genes in a dairy chain and other sources' isolates and also into a geographical distribution of some P-types. We have shown that industrial dairy plants may represent an environmental site favouring a selection of the isolates with a higher pathogenetic pattern. © 2017 The Society for Applied Microbiology.

Physical exercise enhances protein kinase C delta activity and insulin receptor tyrosine phosphorylation in diabetes-prone psammomys obesus.

PubMed

Heled, Yuval; Shapiro, Yair; Shani, Yoav; Moran, Dani S; Langzam, Leah; Braiman, Liora; Sampson, Sanford R; Meyerovitch, Joseph

2003-08-01

We recently reported that physical exercise prevents the progression of type 2 diabetes mellitus in Psammomys obesus, an animal model of nutritionally induced type 2 diabetes mellitus. In the present study we characterized the effect of physical exercise on protein kinase C delta (PKC delta) activity, as a mediator of the insulin-signaling cascade in vivo. Three groups of Psammomys obesus were exposed to a 4-week protocol: high-energy diet (HE/C), high-energy diet and exercise (HE/EX), or low-energy diet (LE/C). None of the animals in the HE/EX group became diabetic, whereas all the animals in the HE/C group became diabetic. After overnight fast, intraperitoneal (IP) insulin (1U) caused a greater reduction in blood glucose levels in the HE/EX and LE/C groups compared to the HE/C group. Tyrosine phosphorylation of insulin receptor (IR), insulin receptor substrate-1 (IRS-1), and phosphatidylinositol 3 kinase (PI3 kinase) was significantly higher in the HE/EX and LE/C groups compared with the HE/C group. Finally, IR-associated PKC delta was higher in the HE/EX and LE/C groups compared to the HE/C group. Coprecipitation of PKC delta with IR was higher in the HE/EX and LE/C groups compared to the HE/C group. Thus, we suggest that 4 weeks of physical exercise results in improved insulin-signaling response in Psammomys obesus accompanied by a direct connection between PKC delta and IR. We conclude that this mechanism may be involved in the preventive effect of exercise on type 2 diabetes mellitus in Psammomys obesus.

Clinical Practices and Outcomes on Chemotherapy-Induced Nausea and Vomiting Management in South Korea: Comparison with Asia-Pacific Data of the Pan Australasian Chemotherapy Induced Emesis Burden of Illness Study

PubMed Central

Lee, Myung Ah; Cho, Eun Kyung; Oh, Sung Yong; Ahn, Joong Bae; Lee, Ji Yun; Thomas, Burke; Jung, Hun; Kim, Jong Gwang

2016-01-01

Purpose This study reported patient outcomes of chemotherapy-induced nausea and vomiting (CINV) prophylaxis for highly emetogenic chemotherapy (HEC) and moderately emetogenic chemotherapy (MEC) regimens and evaluated its adherence to acute-phase CINV prophylaxis in the Korean population subset of the Pan Australasian Chemotherapy Induced Emesis burden of illness (PrACTICE) study. Materials and Methods This subgroup analysis evaluated 158 Korean patients receiving HEC or MEC and compared the data (wherever possible) with that of 648 patients from the Asia-Pacific (AP) region. Study endpoints included evaluation of primary CINV prophylaxis and adherence to acute-phase CINV prophylaxis in cycle 1 (American Society of Clinical Oncology [ASCO] Quality Oncology Practice Initiative [QOPI]). Results In South Korea and the AP, a 5-hydroxytryptamine-3 receptor antagonist (5HT3-RA) prophylaxis for the acute phase was administered to 79/80 patients (98.8%) for HEC and 70/71 patients (98.6%) for MEC regimens (QOPI-1). Triple regimen (corticosteroid–5HT3-RA–neurokinin 1-RA) was initiated in 46/80 patients (57.5%) for prophylaxis of acute CINV in cycle 1 of HEC (QOPI-3). Double regimen (corticosteroid–5HT3-RA, with or within NK1-RA) was initiated in 61/71 patients (83.1%) for control of acute CINV in cycle 1 of MEC a(QOPI-2). Conclusion Active management of CINV is necessary in cycle 1 of HEC in South Korea, despite higher rates than the AP region. Adherence to the international guidelines for CINV prophylaxis requires attention in the acute phase in cycle 1 of the HEC regimen. PMID:26875197

Properties and in vitro drug release of hyaluronic acid-hydroxyethyl cellulose hydrogels for transdermal delivery of isoliquiritigenin.

PubMed

Kong, Bong Ju; Kim, Ayoung; Park, Soo Nam

2016-08-20

In the present study, the properties of hydrogel systems based on hyaluronic acid (HA)-hydroxyethyl cellulose (HEC) were investigated for effective transdermal delivery of isoliquiritigenin (ILTG). Hydrogels were synthesized by chemical cross-linking, and network structures were characterised using scanning electron microscopy (SEM) and surface area analyser. Texture properties and swelling of HA-HEC hydrogels were found to be closely linked to cross-linker concentration and swelling medium. Water in HA-HEC hydrogels was found to exist mostly in the form of free water. The viscoelasticity and the network stabilization of the hydrogels were analysed via rheological studies. The release kinetics of the hydrogel followed Fickian diffusion mechanism. In an in vitro skin penetration study, the system substantially improved the delivery of ILTG into the skin. These results indicate that the hydrogel system composed of HA and HEC has potential as a transdermal delivery system, with cross-linking density and the swelling medium influencing the properties. Copyright © 2016 Elsevier Ltd. All rights reserved.

Cost-utility and budget impact analyses of the use of NEPA for chemotherapy-induced nausea and vomiting prophylaxis in Italy.

PubMed

Restelli, Umberto; Saibene, Gabriella; Nardulli, Patrizia; Di Turi, Roberta; Bonizzoni, Erminio; Scolari, Francesca; Perrone, Tania; Croce, Davide; Celio, Luigi

2017-08-01

To evaluate the efficiency of resources allocation and sustainability of the use of netupitant+palonosetron (NEPA) for chemotherapy-induced nausea and vomiting (CINV) prophylaxis assuming the Italian National Health Service (NHS) perspective. A published Markov model was adapted to assess the incremental cost-utility ratio of NEPA compared with aprepitant (APR) + palonosetron (PALO), fosaprepitant (fAPR) + PALO, APR + ondansetron (ONDA), fAPR + ONDA in patients receiving a highly emetogenic chemotherapy (HEC) and with APR + PALO and fAPR + PALO in patients receiving a moderately emetogenic chemotherapy (MEC). Oncology hospital department in Italy. A Markov model was used to determine the impact of NEPA on the budget of the Italian NHS on a 5-day time horizon, corresponding to the acute and delayed CINV prophylaxis phases. Direct medical costs considered were related to antiemetic drugs, adverse events management, CINV episodes management. Clinical and quality of life data referred to previously published works. The budget impact analysis considered the aforementioned therapies plus PALO alone (for HEC and MEC) on a 5-year time horizon, comparing two scenarios: one considering the use of NEPA and one not considering its use. Incremental cost per quality adjusted life year (QALY) and differential economic impact for the Italian NHS between the two scenarios considered. NEPA is more effective and less expensive (dominant) compared with APR + PALO (for HEC and MEC), fAPR + PALO (for HEC and MEC), APR + ONDA (for HEC), fAPR + ONDA (for HEC). The use of NEPA would lead to a 5-year cost decrease of €63.7 million (€42.7 million for HEC and €20.9 million for MEC). NEPA allows an efficient allocation of resources for the Italian NHS and it is sustainable, leading to a cost decrease compared with a scenario which does not consider its use. © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2017. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

H19 promotes endometrial cancer progression by modulating epithelial-mesenchymal transition

PubMed Central

Zhao, Le; Li, Zhen; Chen, Wei; Zhai, Wen; Pan, Jingjing; Pang, Huan; Li, Xu

2017-01-01

Endometrial cancer is one of the most common types of gynecological malignancy worldwide. Novel biomarkers and therapeutic targets are imperative for improving patients' survival. Previous studies have suggested the long non-coding RNA H19 as a potential cancer biomarker. To investigate the role of H19 in endometrial cancer, the present study examined the expression pattern of H19 in endometrial cancer tissues by quantitative polymerase chain reaction, and characterized its function in the endometrial cancer cell line via knocking down its expression with small interfering RNAs. It was found that H19 level was significantly higher in tumor tissues than in paratumoral tissues. Knockdown of H19 did not affect the growth rate of HEC-1-B endometrial cancer cells, but significantly suppressed in vitro migration and invasion of HEC-1-B cells. Furthermore, H19 downregulation decreased Snail level and increased E-cadherin expression without affecting vimentin level, indicating partial reversion of epithelial-mesenchymal transition (EMT). The present findings suggested that H19 contributed to the aggressiveness of endometrial cancer by modulating EMT process. PMID:28123568

77 FR 56581 - Airworthiness Directives; Sikorsky Aircraft Corporation Helicopters

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-13

... HEC, in this model helicopter that now meets the Category A performance standard. DATES: We must... airworthiness directive (AD) for the Sikorsky Aircraft Corporation (Sikorsky) Model S-92A helicopter, which... external load operations, including human external cargo (HEC), because this model helicopter was not...

Flood Risk and Asset Management

DTIC Science & Technology

2011-06-15

Model cascade could include HEC - RAS , HR BREACH and Dynamic RFSM. Action HRW to consider model coupling and advise DM. It was felt useful to...simple loss of life approach. WL can provide input and advise on USACE LIFESIM approaches. To enable comparison with HEC FRM approaches, it was

Impact of periodic health examination on surgical treatment for uterine fibroids in Beijing: a case-control study.

PubMed

Wu, Hai-Yun; Yang, Ling-Ling; Zhou, Shan

2010-12-07

During the past 2 decades, there has been a rapid proliferation of "health examination center (HEC)" across China. The effects of their services on public's health have not been systemically investigated. This study aimed to assess the impact of periodic health examination (PHE) at HEC on surgical treatment for uterine fibroids in Beijing residents. We identified 224 patients with a primary diagnosis of uterine fibroids who had surgical treatment at four Level-1 general hospitals in Beijing, from June 1, 2009 to October 20, 2009. Controls were women who did not have surgery for uterine fibroids, matched (1:1 ratio) for age (within 2 years). A standard questionnaire was used to inquire about whether participants had PHE at HEC during the previous 2 years. PHE at HEC within 2 years were associated with surgical treatment for uterine fibroids. Odds ratios was 4.05 (95% CI, 2.61-6.29 P < 0.001), after adjustment for marital status, whether have children, annual family income, health insurance, education level and self-rated uterine fibroids-related symptom severity. Our study showed PHE currently provided at HEC in China were associated with significantly increased use of surgical treatment for uterine fibroids in women. Further studies are needed to assess the effects of PHE on clinical as well as on broad societal outcomes in Chinese in contemporary medical settings.

Møller-Plesset perturbation energies and distances for HeC(20) extrapolated to the complete basis set limit.

PubMed

Varandas, A J C

2009-02-01

The potential energy surface for the C(20)-He interaction is extrapolated for three representative cuts to the complete basis set limit using second-order Møller-Plesset perturbation calculations with correlation consistent basis sets up to the doubly augmented variety. The results both with and without counterpoise correction show consistency with each other, supporting that extrapolation without such a correction provides a reliable scheme to elude the basis-set-superposition error. Converged attributes are obtained for the C(20)-He interaction, which are used to predict the fullerene dimer ones. Time requirements show that the method can be drastically more economical than the counterpoise procedure and even competitive with Kohn-Sham density functional theory for the title system.

Comparison of Dam Breach Parameter Estimators

DTIC Science & Technology

2008-01-01

of the methods, when used in the HEC - RAS simulation model , produced comparable results. The methods tested suggest use of ...characteristics of a dam breach, use of those parameters within the unsteady flow routing model HEC - RAS , and the computation and display of the resulting...implementation of these breach parameters in

Hydraulic Characteristics of Bedrock Constrictions and Evaluation of One- and Two-Dimensional Models of Flood Flow on the Big Lost River at the Idaho National Engineering and Environmental Laboratory, Idaho

USGS Publications Warehouse

Berenbrock, Charles; Rousseau, Joseph P.; Twining, Brian V.

2007-01-01

A 1.9-mile reach of the Big Lost River, between the Idaho National Engineering and Environmental Laboratory (INEEL) diversion dam and the Pioneer diversion structures, was investigated to evaluate the effects of streambed erosion and bedrock constrictions on model predictions of water-surface elevations. Two one-dimensional (1-D) models, a fixed-bed surface-water flow model (HEC-RAS) and a movable-bed surface-water flow and sediment-transport model (HEC-6), were used to evaluate these effects. The results of these models were compared to the results of a two-dimensional (2-D) fixed-bed model [Transient Inundation 2-Dimensional (TRIM2D)] that had previously been used to predict water-surface elevations for peak flows with sufficient stage and stream power to erode floodplain terrain features (Holocene inset terraces referred to as BLR#6 and BLR#8) dated at 300 to 500 years old, and an unmodified Pleistocene surface (referred to as the saddle area) dated at 10,000 years old; and to extend the period of record at the Big Lost River streamflow-gaging station near Arco for flood-frequency analyses. The extended record was used to estimate the magnitude of the 100-year flood and the magnitude of floods with return periods as long as 10,000 years. In most cases, the fixed-bed TRIM2D model simulated higher water-surface elevations, shallower flow depths, higher flow velocities, and higher stream powers than the fixed-bed HEC-RAS and movable-bed HEC-6 models for the same peak flows. The HEC-RAS model required flow increases of 83 percent [100 to 183 cubic meters per second (m3/s)], and 45 percent (100 to 145 m3/s) to match TRIM2D simulations of water-surface elevations at two paleoindicator sites that were used to determine peak flows (100 m3/s) with an estimated return period of 300 to 500 years; and an increase of 13 percent (150 to 169 m3/s) to match TRIM2D water-surface elevations at the saddle area that was used to establish the peak flow (150 m3/s) of a paleoflood with a return period of 10,000 years. A field survey of the saddle area, however, indicated that the elevation of the lowest point on the saddle area was 1.2 feet higher than indicated on the 2-ft contour map that was used in the TRIM2D model. Because of this elevation discrepancy, HEC-RAS model simulations indicated that a peak flow of at least 210 m3/s would be needed to initiate flow across the 10,000-year old Pleistocene surface. HEC-6 modeling results indicated that to compensate for the effects of streambed scour, additional flow increases would be needed to match HEC-RAS and TRIM2D water-surface elevations along the upper and middle reaches of the river, and to compensate for sediment deposition, a slight decrease in flows would be needed to match HEC-RAS water-surface elevations along the lower reach of the river. Differences in simulated water-surface elevations between the TRIM2D and the HEC-RAS and HEC-6 models are attributed primarily to differences in topographic relief and to differences in the channel and floodplain geometries used in these models. Topographic differences were sufficiently large that it was not possible to isolate the effects of these differences on simulated water-surface elevations from those attributable to the effects of supercritical flow, streambed scour, and sediment deposition.

Expression and Functional Significance of HtrA1 Loss in Endometrial Cancer

PubMed Central

Mullany, Sally A.; Moslemi-Kebria, Mehdi; Rattan, Ramandeep; Khurana, Ashwani; Clayton, Amy; Ota, Takayo; Mariani, Andrea; Podratz, Karl C.; Chien, Jeremy; Shridhar, Viji

2010-01-01

Purpose The purpose of this study was to determine if loss of serine protease HtrA1 in endometrial cancer will promote the invasive potential of EC cell lines. Experimental design Western blot analysis and immunohistochemistry methods were used to determine HtrA1 expression in EC cell lines and primary tumors, respectively. Migration, invasion assays and in vivo xenograft experiment were performed to compare the extent of metastasis between HtrA1 expressing and HtrA-1 knocked down clones. Results Western blot analysis of HtrA1 in 13 EC cell lines revealed complete loss of HtrA1 expression in all 7 papillary serous EC cell lines. Downregulation of HtrA1 in Hec1A and Hec1B cell lines resulted in a 3-4 fold increase in the invasive potential. Exogenous expression of HtrA1 in Ark 1 and Ark 2 cells resulted in 3-4 fold decrease in both invasive and migration potential of these cells. There was an increased rate of metastasis to the lungs associated with HtrA1 downregulation in Hec1B cells compared to control cells with endogenous HtrA1 expression. Enhanced expression of HtrA1 in Ark 2 cells resulted in significantly less tumor nodules metastasizing to the lungs compared to parental or protease deficient (SA mutant) Ark 2 cells. Immunohistochemical (IHC) analysis showed 57% (105/184) of primary EC tumors had low HtrA1 expression. The association of low HtrA1 expression with high-grade endometrioid tumors was statistically significant (p=0.016). Conclusions Collectively, these data indicate loss of HtrA1 may contribute to the aggressiveness and metastatic ability of endometrial tumors. PMID:21098697

HPMC supplementation reduces abdominal fat content, intestinal permeability, inflammation, and insulin resistance in diet-induced obese mice

USDA-ARS?s Scientific Manuscript database

Cationic hydroxyethyl cellulose (cHEC) , was fed to hamsters to determine if this new soluble fiber had an effect on hypercholesterolemia and dyslipidemia associated with cardiovascular disease. In this study, Golden Syrian hamsters were supplemented with 3-8% cHEC or microcrystalline cellulose (MC...

Does Homeownership Counseling Affect the Prepayment and Default Behavior of Affordable Mortgage Borrowers?

ERIC Educational Resources Information Center

Quercia, Roberto; Spader, Jonathan

2008-01-01

The lack of industry wide data on homeownership education and counseling (HEC) programs has severely limited evaluation. In particular, very little evidence exists on the relationship between HEC completion and loan prepayment, an outcome of interest to both mortgage lenders and consumer advocates. Where mortgage prepayment directly influences the…

Stimuli sensitive super-macroporous cryogels based on photo-crosslinked 2-hydroxyethylcellulose and chitosan.

PubMed

Stoyneva, Veselina; Momekova, Denitsa; Kostova, Bistra; Petrov, Petar

2014-01-01

Original pH sensitive cryogels, based on two biodegradable natural polymers chitosan (CS) and 2-hydroxyethylcellulose (HEC), were obtained via cryogenic treatment of semi-dilute aqueous solutions and UV induced crosslinking in frozen state. H₂O₂ and N,N'-methylenebisacrylamide (BisAAm) were used as photoinitiator and crosslinking agent, respectively. BisAAm facilitated the formation of polymer co-network and increased both the gel fraction yield and mechanical strength of cryogels. The influence of chitosan content on the physico-mechanical properties of HEC-CS cryogels was investigated. In general, the increase of CS fraction in the polymer co-network increased the degree of swelling and enhanced significantly the storage modulus of materials. All HEC-CS cryogels obtained were opalescent sponge-like materials, which quickly release/uptake water due to their open porous structure. The incorporation of CS provided pH dependent swelling and good bioadhesive properties of cryogels. HEC-CS cryogels were further exploited as drug delivery systems of the highly water soluble drug metronidazole belonging to BCS Class l. Copyright © 2013 Elsevier Ltd. All rights reserved.

Development of curcumin-loaded poly(hydroxybutyrate- co-hydroxyvalerate) nanoparticles as anti-inflammatory carriers to human-activated endothelial cells

NASA Astrophysics Data System (ADS)

Simion, Viorel; Stan, Daniela; Gan, Ana-Maria; Pirvulescu, Monica Madalina; Butoi, Elena; Manduteanu, Ileana; Deleanu, Mariana; Andrei, Eugen; Durdureanu-Angheluta, Anamaria; Bota, Marian; Enachescu, Marius; Calin, Manuela; Simionescu, Maya

2013-12-01

Curcumin (Cm)-loaded poly(hydroxybutyrate- co-hydroxyvalerate) (PHBV) nanoparticles (CmPN) were obtained and characterized and their effect on human endothelial cells (HEC) was assessed. Different CmPN formulations have been prepared using the emulsion solvent evaporation technique, and characterized for size, structure, Zeta potential, Cm entrapment efficiency, and in vitro Cm release. CmPN cytotoxicity and cellular uptake have been followed using HEC. Also, the effect of CmPN treatment on the p38MAPK signaling pathway in endothelial cells was investigated. The results obtained by electron and atomic force microscopy revealed the spherical shape of the CmPN formulation. Based on size and encapsulation efficiency, the CmPN formulation with the average diameter of 186 nm and with the highest encapsulation efficiency (83 %) has been used in the further studies. The release of Cm from CmPN was 18 % after 8 h of incubation at 37 °C, followed by a slow release until 144 h, when it reached 44 %, indicating a controlled release. CmPN are taken up by HEC and exhibited low cytotoxicity at concentrations up to 10 μM. The pre-treatment of HEC with CmPN before exposure to tumor necrosis factor-alpha (TNF-α) determined a decrease of p38MAPK phosphorylation. In conclusion, Cm encapsulated into PHBV nanoparticles, at concentration up to 10 μM, has low cytotoxicity and display anti-inflammatory activity on TNF-α-activated HEC by suppressing the phosphorylation of p38MAPK.

Hydrologic modeling of Guinale River Basin using HEC-HMS and synthetic aperture radar

NASA Astrophysics Data System (ADS)

Bien, Ferdinand E.; Plopenio, Joanaviva C.

2017-09-01

This paper presents the methods and results of hydrologic modeling of Guinale river basin through the use of HEC-HMS software and Synthetic Aperture Radar Digital Elevation Model (SAR DEM). Guinale River Basin is located in the province of Albay, Philippines which is one of the river basins covered by the Ateneo de Naga University (ADNU) Phil-LiDAR 1. This research project was funded by the Department of Science and Technology (DOST) through the Philippine Council for Industry, Energy and Emerging Technology Research and Development (PCIEERD). Its objectives are to simulate the hydrologic model of Guinale River basin using HEC-HMS software and SAR DEM. Its basin covers an area of 165.395 sq.km. and the hydrologic model was calibrated using the storm event typhoon Nona (international name Melor). Its parameter had undergone a series of optimization processes of HEC-HMS software in order to produce an acceptable level of model efficiency. The Nash-Sutcliffe (E), Percent Bias and Standard Deviation Ratio were used to measure the model efficiency, giving values of 0.880, 0.260 and 0.346 respectively which resulted to a "very good" performance rating of the model. The flood inundation model was simulated using Legazpi Rainfall Intensity Duration Frequency Curves (RIDF) and HEC-RAS software developed by the US Army corps of Engineers (USACE). This hydrologic model will provide the Municipal Disaster Risk Reduction Management Office (MDRRMO), Local Government units (LGUs) and the community a tool for the prediction of runoff in the area.

The Anticancer Effects of Radachlorin-mediated Photodynamic Therapy in the Human Endometrial Adenocarcinoma Cell Line HEC-1-A.

PubMed

Kim, Su-Mi; Rhee, Yun-Hee; Kim, Jong-Soo

2017-11-01

We investigated the effect of photodynamic therapy (PDT) using radachlorin on invasion, vascular formation and apoptosis by targeting epidermal growth factor receptor (EGFR)/vascular endothelial growth factor receptor 2 (VEGFR2) signaling pathways in the HEC-1-A endometrial adenocarcinoma cell line. To investigate the apoptotic pathway, we performed the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay, and western blot analysis. We also evaluated the effects of PDT on tubular capillary formation in and invasion by HEC-1-A cells with a tube formation assay, invasion assay, prostaglandin E2 (PGE2) assay, and western blot analysis. PDT had anticancer effects on HEC-1-A through activation of the intrinsic pathway of apoptosis via caspase-9 and poly-(ADP-ribose) polymerase (PARP). PDT also inhibited tubular capillary formation in and invasion by HEC-1-A under VEGF pretreatment, that resulted from down-regulation of VEGFR2, EGFR, Ras homolog gene family/ member A (RhoA) and PGE2. These results are indicative of the specificity of radachlorin-mediated PDT to VEGF. The major advantage of radachlorin-mediated PDT is its selectivity for cancer tissue while maintaining adjacent normal endometrial tissue. Therefore, radachlorin-mediated PDT might offer high anticancer efficacy for endometrial adenocarcinoma and an especially useful modality for preserving fertility. Copyright© 2017, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

Sleep Disruption is Associated with Increased Ventricular Ectopy and Cardiac Arrest in Hospitalized Adults

PubMed Central

Miner, Steven Edward Stuart; Pahal, Dev; Nichols, Laurel; Darwood, Amanda; Nield, Lynne Elizabeth; Wulffhart, Zaev

2016-01-01

Study Objectives: To determine whether sleep disruption increases ventricular ectopy and the risk of cardiac arrest in hospitalized patients. Methods: Hospital emergency codes (HEC) trigger multiple hospital-wide overhead announcements. In 2014 an electronic “code white” program was instituted to protect staff from violent patients. This resulted in an increase in nocturnal HEC. Telemetry data was examined between September 14 and October 2, 2014. The frequency of nocturnal announcements was correlated with changes in frequency of premature ventricular complexes per hour (PVC/h). Cardiac arrest data were examined over a 3-y period. All HEC were assumed to have triggered announcements. The relationship between nocturnal HEC and the incidence of subsequent cardiac arrest was examined. Results: 2,603 hours of telemetry were analyzed in 87 patients. During nights with two or fewer announcements, PVC/h decreased 33% and remained 30% lower the next day. On nights with four or more announcements, PVC/h increased 23% (P < 0.001) and further increased 85% the next day (P = 0.001). In 2014, following the introduction of the code white program, the frequency of all HEC increased from 1.1/day to 6.2/day (P < 0.05). The frequency of cardiac arrest/24 h rose from 0.46/day in 2012–2013 to 0.62/day in 2014 (P = 0.001). During daytime hours (06:00–22:00), from 2012 through 2014, the frequency of cardiac arrest following zero, one or at least two nocturnal HEC were 0.331 ± 0.03, 0.396 ± 0.04 and 0.471 ± 0.09 respectively (R2 = 0.99, P = 0.03). Conclusions: Sleep disruption is associated with increased ventricular ectopy and increased frequency of cardiac arrest. Citation: Miner SE, Pahal D, Nichols L, Darwood A, Nield LE, Wulffart Z. Sleep disruption is associated with increased ventricular ectopy and cardiac arrest in hospitalized adults. SLEEP 2016;39(4):927–935. PMID:26715226

Impact of 5-HT(3) RA selection within triple antiemetic regimens on uncontrolled highly emetogenic chemotherapy-induced nausea/vomiting.

PubMed

Schwartzberg, Lee; Jackson, James; Jain, Gagan; Balu, Sanjeev; Buchner, Deborah

2011-08-01

It is recommended that patients initiate triple antiemetic therapy with one of the 5-hydroxytryptamine receptor antagonists (5-HT(3) RAs), aprepitant (or its intravenous prodrug fosaprepitant) and dexamethasone prior to the start of highly emetogenic chemotherapy (HEC). However, the impact of 5-HT(3) RA selection within triple antiemetic regimens on the risk of uncontrolled chemotherapy-induced nausea and vomiting (CINV) with HEC has not been well studied. To assess the likelihood of an uncontrolled CINV event following antiemetic prophylaxis with the 5-HT(3) RA palonosetron + aprepitant/fosaprepitant + dexamethasone (palonosetron cohort) versus any of the other 5-HT(3) RAs + aprepitant/fosaprepitant + dexamethasone (other 5-HT(3) RA cohort) among single-day HEC cycles. Single-day HEC cycles (a gap of at least 5 days between two administrations) among patients with a cancer diagnosis and receiving antiemetic prophylaxis with the aforementioned regimens between 1/1/2006 and 6/30/2010 were identified from the IMS LifeLink claims database. Uncontrolled CINV events were identified through ICD-9-CM codes (nausea and vomiting), Current Procedural Terminology codes (hydration), rescue medications and/or use of antiemetic therapy from days 2-5 following HEC administration. Risks for an uncontrolled CINV event among all patients, and within breast cancer and multiple cancer subpopulations, were analyzed at cycle level using logistic multivariate regression models. A total of 8018 cycles for the palonosetron cohort and 1926 cycles for the other 5-HT(3) RA cohort (3574 and 978 patients, respectively) were analyzed. Single-day HEC cycles received by the palonosetron cohort had a significantly lower unadjusted risk of an uncontrolled CINV event (17.5 vs 20.7% for the other 5-HT(3) RA cohort; p = 0.0010), with a 17% lower adjusted risk for palonosetron-administered cycles (odds ratio: 0.83; 95% CI: 0.73-0.94; p = 0.0042). Results in the breast cancer and multiple cancer subgroups were consistent with those for the overall population. In this retrospective claims data analysis, single-day HEC cycles administered with palonosetron + aprepitant/fosaprepitant + dexamethasone had a lower risk for an uncontrolled CINV event versus other 5-HT(3) RAs + aprepitant/fosaprepitant + dexamethasone.

Flood plain analysis for Petris, , Troas, and Monoros, tia watersheds, the Arad department, Romania

NASA Astrophysics Data System (ADS)

Győri, M.-M.; Haidu, I.

2012-04-01

The present study sets out to determine the flood plains corresponding to flood discharges having 10, 50 and 100 year recurrence intervals on the Monoroštia, Petriš and Troaš Rivers, located in Western Romania, the Arad department. The data of the study area is first collected and pre-processed in ArcGIS. It consists of land use data, soil data, the DEM, stream gauges' and meteorological stations' locations, on the basis of which the watersheds' hydrologic parameters' are computed using the Geospatial Hydrologic Modelling Extension (HEC Geo-HMS). HEC Geo-HMS functions as an interface between ArcGIS and HEC-HMS (Hydrologic Engineering Centre- Hydrologic Modelling System) and converts the data collected and generated in ArcGIS to data useable by HEC-HMS. The basin model component in HEC-HMS represents the physical watershed. It facilitates the effective rainfall computation on the basis of the input hyetograph, passing the results to a transform function that converts the excess precipitation into runoff at the subwatersheds' outlet. This enables the estimation and creation of hydrographs for the ungauged watersheds. In the present study, the results are achieved through the SCS CN loss method and the SCS Unit hydrograph transform method. The simulations use rainfall data that is registered at the stations situated in the catchments' vicinity, data that spans over two decades (1989-2009) and which allows the rainfall hyetographs to be determined for the above mentioned return periods. The model will be calibrated against measured streamflow data from the gauging stations on the main rivers, leading to the adjustment of watershed parameters, such as the CN parameter. As the flood discharges for 10, 50 and 100 year return periods have been determined, the profile of the water surface elevation along the channel will be computed through a steady flow analysis, with HEC-RAS (Hydrologic Engineering Centre- River Analysis System). For each of the flood frequencies, a water surface TIN is generated and intersected with the DEM in order to create the flood plain polygons. The final result consists of the flood plain delineation and the water inundation depths for the 10, 50 and 100 year return period flood events. These could be further employed in a risk assessment. Key words : flood plain analysis, frequency analysis, HEC-HMS, HEC-RAS. Aknowledgements This work was possible with the financial support of the Sectoral Operational Programme for Human Resources Development 2007-2013, co-financed by the European Social Fund, under the project number POSDRU/107/1.5/S/76841 with the title "Modern Doctoral Studies: Internationalization and Interdisciplinarity".

ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE1 (ADPG1), ADPG2, and QUARTET2 Are Polygalacturonases Required for Cell Separation during Reproductive Development in Arabidopsis[W

PubMed Central

Ogawa, Mikihiro; Kay, Pippa; Wilson, Sarah; Swain, Stephen M.

2009-01-01

Cell separation is thought to involve degradation of pectin by several hydrolytic enzymes, particularly polygalacturonase (PG). Here, we characterize an activation tagging line with reduced growth and male sterility caused by increased expression of a PG encoded by QUARTET2 (QRT2). QRT2 is essential for pollen grain separation and is part of a small family of three closely related endo-PGs in the Arabidopsis thaliana proteome, including ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE1 (ADPG1) and ADPG2. Functional assays and complementation experiments confirm that ADPG1, ADPG2, and QRT2 are PGs. Genetic analysis demonstrates that ADPG1 and ADPG2 are essential for silique dehiscence. In addition, ADPG2 and QRT2 contribute to floral organ abscission, while all three genes contribute to anther dehiscence. Expression analysis is consistent with the observed mutant phenotypes. INDEHISCENT (IND) encodes a putative basic helix-loop-helix required for silique dehiscence, and we demonstrate that the closely related HECATE3 (HEC3) gene is required for normal seed abscission and show that IND and HEC3 are required for normal expression of ADPG1 in the silique dehiscence zone and seed abscission zone, respectively. We also show that jasmonic acid and ethylene act together with abscisic acid to regulate floral organ abscission, in part by promoting QRT2 expression. These results demonstrate that multiple cell separation events, including both abscission and dehiscence, require closely related PG genes. PMID:19168715

Important roles of the AKR1C2 and SRD5A1 enzymes in progesterone metabolism in endometrial cancer model cell lines.

PubMed

Sinreih, Maša; Anko, Maja; Zukunft, Sven; Adamski, Jerzy; Rižner, Tea Lanišnik

2015-06-05

Endometrial cancer is the most frequently diagnosed gynecological malignancy. It is associated with prolonged exposure to estrogens that is unopposed by progesterone, whereby enhanced metabolism of progesterone may decrease its protective effects, as it can deprive progesterone receptors of their active ligand. Furthermore, the 5α-pregnane metabolites formed can stimulate proliferation and may thus contribute to carcinogenesis. The aims of our study were to: (1) identify and quantify progesterone metabolites formed in the HEC-1A and Ishikawa model cell lines of endometrial cancer; and (2) pinpoint the enzymes involved in progesterone metabolism, and delineate their roles. Progesterone metabolism studies combined with liquid chromatography-tandem mass spectrometry enabled identification and quantification of the metabolites formed in these cells. Further quantitative PCR analysis and small-interfering-RNA-mediated gene silencing identified individual progesterone metabolizing enzymes and their relevant roles. In Ishikawa and HEC-1A cells, progesterone was metabolized mainly to 20α-hydroxy-pregn-4-ene-3-one, 20α-hydroxy-5α-pregnane-3-one, and 5α-pregnane-3α/β,20α-diol. The major difference between these cell lines was rate of progesterone metabolism, which was faster in HEC-1A cells. In the Ishikawa and HEC-1A cells, expression of AKR1C2 was 110-fold and 6800-fold greater, respectively, than expression of AKR1C1, which suggests that 20-ketosteroid reduction of 5α-pregnanes and 4-pregnenes is catalyzed mainly by AKR1C2. AKR1C1/AKR1C2 gene silencing showed decreased progesterone metabolism in both cell lines, thus further supporting the significant role of AKR1C2. SRD5A1 was also expressed in these cells, and its silencing confirmed that 5α-reduction is catalyzed by 5α-reductase type 1. Silencing of SRD5A1 also had the most pronounced effects, with decreased rate of progesterone metabolism, and consequently higher concentrations of unmetabolized progesterone. Our data confirm that in model cell lines of endometrial cancer, AKR1C2 and SRD5A1 have crucial roles in progesterone metabolism, and may represent novel targets for treatment. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

Light scattering experiments on aqueous solutions of selected cellulose ethers: contribution to the study of polymer-mineral interactions in a new injectable biomaterial.

PubMed

Bohic, S; Weiss, P; Roger, P; Daculsi, G

2001-03-01

Hydroxypropylmethylcellulose (HPMC) is used as a ligand for a bioactive calcium phosphate ceramic (the filler) in a ready-to-use injectable sterilized biomaterial for bone and dental surgery. Light scattering experiments were usually used to study high water-soluble polymers and to determine the basic macromolecular parameters. In order to gain a deeper understanding of polymer/mineral interactions in this type of material, we have investigated the effect of divalent and trivalent ions (Ca(2+), PO(4)(3-)) and steam sterilization on dilute solutions of HPMC and hydroxyethylcellulose (HEC). The sterilization process may cause some degradation of HEC taking into account its high molecular weight and some rigidity of the polymer chain. Moreover, in the case of HPMC, the changes in the conformations rather than degradation process are supposed. These effects of degradation and flocculation are strengthened in alkaline medium. Experimental data suggested the formation of chelate complexes between Ca(2+) and HPMC which improve its affinity to the mineral blend and consolidate the injectable biomaterial even in the case of its hydration by biological fluid. Copyright 2001 Kluwer Academic Publishers

Intestinal Targeting of Ganciclovir Release Employing a Novel HEC-PAA Blended Lyomatrix.

PubMed

Mabrouk, Mostafa; Mulla, Jameel A S; Kumar, Pradeep; Chejara, Dharmesh R; Badhe, Ravindra V; Choonara, Yahya E; du Toit, Lisa C; Pillay, Viness

2016-10-01

A hydroxyethylcellulose-poly(acrylic acid) (HEC-PAA) lyomatrix was developed for ganciclovir (GCV) intestine targeting to overcome its undesirable degradation in the stomach. GCV was encapsulated within the HEC-PAA lyomatrix prepared by lyophilization. Conventional tablets were also prepared with identical GCV concentrations in order to compare the GCV release behavior from the lyomatrix and tablets. GCV incorporation (75.12%) was confirmed using FTIR, DSC, and TGA. The effect of GCV loading on the microstructure properties of the lyomatrix was evaluated by SEM, AFM, and BET surface area measurements. The in vitro drug release study showed steady and rapid release profiles from the GCV-loaded lyomatrix compared with the tablet formulation at identical pH values. Minimum GCV release was observed at acidic pH (≤40%) and maximum release occurred at intestinal pH values (≥90%) proving the intestinal targeting ability of the lyomatrix. Kinetic modeling revealed that the GCV-loaded lyomatrix exhibited zero-order release kinetics (n = 1), while the tablets were best described via the Peppas model. Textural analysis highlighted enhanced matrix resilience and rigidity gradient (12.5%, 20 Pa) for the GCV-loaded lyomatrix compared to the pure (7%, 9.5 Pa) HEC-PAA lyomatrix. Bench-top MRI imaging was used to confirm the mechanism of GCV release behavior by monitoring the swelling and erosion rates. The swelling and erosion rate of the tablets was not sufficient to achieve rapid zero-order GCV release as with the lyomatrix. These combined results suggest that the HEC-PAA lyomatrix may be suitable for GCV intestinal targeting after oral administration.

Inhibition of angiogenesis by vitamin D-binding protein: characterization of anti-endothelial activity of DBP-maf.

PubMed

Kalkunte, Satyan; Brard, Laurent; Granai, Cornelius O; Swamy, Narasimha

2005-01-01

Angiogenesis is a complex process involving coordinated steps of endothelial cell activation, proliferation, migration, tube formation and capillary sprouting with participation of intracellular signaling pathways. Regulation of angiogenesis carries tremendous potential for cancer therapy. Our earlier studies showed that vitamin D-binding protein-macrophage activating factor (DBP-maf) acts as a potent anti-angiogenic factor and inhibits tumor growth in vivo. The goal of this investigation was to understand the effect of DBP-maf on human endothelial cell (HEC) and the mechanism of angiogenesis inhibition. DBP-maf inhibited human endothelial cell (HEC) proliferation by inhibiting DNA synthesis (IC(50) = 7.8 +/- 0.15 microg/ml). DBP-maf significantly induced S- and G0/G1-phase arrest in HEC in 72 h. DBP-maf potently blocked VEGF-induced migration, tube-formation of HEC in a dose dependent manner. In addition, DBP-maf inhibited growth factor-induced microvessel sprouting in rat aortic ring assay. Moreover, DBP-maf inhibited VEGF signaling by decreasing VEGF-mediated phosphorylation of VEGFR-2 and ERK1/2, a downstream target of VEGF signaling cascade. However, Akt activation was not affected. These studies collectively demonstrate that DBP-maf inhibits angiogenesis by blocking critical steps such as HEC proliferation, migration, tube formation and microvessel sprouting. DBP-maf exerts its effect by inhibiting VEGR-2 and ERK1/2 signaling cascades. Understanding the cellular and molecular mechanisms of anti-endothelial activity of DBP-maf will allow us to develop it as an angiogenesis targeting novel drug for tumor therapy.

Community-based human–elephant conflict mitigation: The value of an evidence-based approach in promoting the uptake of effective methods

PubMed Central

Gunaryadi, Donny; Sugiyo

2017-01-01

Human–elephant conflict (HEC) is a serious threat to elephants and can cause major economic losses. It is widely accepted that reduction of HEC will often require community-based methods for repelling elephants but there are few tests of such methods. We tested community-based crop-guarding methods with and without novel chili-based elephant deterrents and describe changes in farmers’ willingness to adopt these methods following our demonstration of their relative effectiveness. In three separate field-trials that took place over almost two years (October 2005 –May 2007) in two villages adjacent to Way Kambas National Park (WKNP) in Indonesia, we found that community-based crop-guarding was effective at keeping Asian elephants (Elephas maximus) out of crop fields in 91.2% (52 out of 57), 87.6% (156 out of 178), and 80.0% (16 out of 20) of attempted raids. Once the method had been shown to be effective at demonstration sites, farmers in 16 villages around WKNP voluntarily adopted it during the July 2008 to March 2009 period and were able to repel elephants in 73.9% (150 out of 203) of attempted raids, with seven villages repelling 100% of attempted raids. These 16 villages had all experienced high levels of HEC in the preceding years; e.g. they accounted for >97% of the 742 HEC incidents recorded for the entire park in 2006. Our work shows, therefore, that a simple evidence-based approach can facilitate significant reductions in HEC at the protected area scale. PMID:28510590

Performance Analysis of AeroRP with Ground Station Advertisements

DTIC Science & Technology

2012-03-12

results showed that AeroRP outperforms the traditional MANET routing protocols in terms of throughput and packet delivery ra - tio (PDR) [5, 6]. AeroRP...and waiting for the source to re- send the packet increases the end-to-end delay. The AeroNP corruption indicator and HEC -CRC (header error check...Dev ID | NP HEC CRC-16 | +-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+ \\ \\ / AeroTP Payload

Chk1 and Mps1 jointly regulate correction of merotelic kinetochore attachments.

PubMed

Petsalaki, Eleni; Zachos, George

2013-03-01

If uncorrected, merotelic kinetochore attachments can induce mis-segregated chromosomes in anaphase. We show that checkpoint kinase 1 (Chk1) protects vertebrate cells against merotelic attachments and lagging chromosomes and is required for correction of merotelic attachments during a prolonged metaphase. Decreased Chk1 activity leads to hyper-stable kinetochore microtubules, unstable binding of MCAK, Kif2b and Mps1 to centromeres or kinetochores and reduced phosphorylation of Hec1 by Aurora-B. Phosphorylation of Aurora-B at serine 331 (Ser331) by Chk1 is high in prometaphase and decreases significantly in metaphase cells. We propose that Ser331 phosphorylation is required for optimal localization of MCAK, Kif2b and Mps1 to centromeres or kinetochores and for Hec1 phosphorylation. Furthermore, inhibition of Mps1 activity diminishes initial recruitment of MCAK and Kif2b to centromeres or kinetochores, impairs Hec1 phosphorylation and exacerbates merotelic attachments in Chk1-deficient cells. We propose that Chk1 and Mps1 jointly regulate Aurora-B, MCAK, Kif2b and Hec1 to correct merotelic attachments. These results suggest a role for Chk1 and Mps1 in error correction.

Stable and pH-responsive core-shell nanoparticles based on HEC and PMAA networks via template copolymerization

NASA Astrophysics Data System (ADS)

Zhang, Y.; Jin, Q.; Chen, Y.; Zhao, J.

2011-10-01

Taking advantage of the specific hydrogen bonding interactions, stable and pH-responsive core-shell nanoparticles based on hydroxyethyl cellulose (HEC) and polymethacrylic acid (PMAA) networks, with a < D h > size ranging from 190 to 250 nm, can be efficiently prepared via facile one-step co-polymerization of methacrylic acid (MAA) and N, N'-methylenebisacrylamide (MBA) on HEC template in water. Using dynamic light scattering, electrophoretic light scattering, fluorescence spectrometry, thermo-gravimetric analysis, TEM, and AFM observations, the influence of crosslinker MBA as well as the reaction parameters were studied. The results show that after the introduction of crosslinker MBA, the nanoparticles became less compact; their size exhibited a smaller pH sensitivity, and their stability against pH value was improved greatly. Furthermore, the size, structure, and pH response of the nanoparticles can be adjusted via varying the reaction parameters: nanoparticles of smaller size, more compact structure, and higher swelling capacity were produced as pH value of the reaction medium increased or the HEC/MAA ratio decreased; while nanoparticles of smaller size, less compact structure and smaller swelling capacity were produced as the total feeding concentration increased.

Specific knockdown of Oct4 and beta2-microglobulin expression by RNA interference in human embryonic stem cells and embryonic carcinoma cells.

PubMed

Matin, Maryam M; Walsh, James R; Gokhale, Paul J; Draper, Jonathan S; Bahrami, Ahmad R; Morton, Ian; Moore, Harry D; Andrews, Peter W

2004-01-01

We have used RNA interference (RNAi) to downregulate beta2-microglobulin and Oct4 in human embryonal carcinoma (hEC) cells and embryonic stem (hES) cells, demonstrating that RNAi is an effective tool for regulating specific gene activity in these human stem cells. The knockdown of Oct4 but not beta2-microglobulin expression in both EC and ES cells resulted in their differentiation, as indicated by a marked change in morphology, growth rate, and surface antigen phenotype, with respect to SSEA1, SSEA3, and TRA-1-60 expression. Expression of hCG and Gcm1 was also induced following knockdown of Oct4 expression, in both 2102Ep hEC cells and in H7 and H14 hES cells, consistent with the conclusion that, as in the mouse, Oct4 is required to maintain the undifferentiated stem cell state, and that differentiation to trophectoderm occurs in its absence. NTERA2 hEC cells also differentiated, but not to trophectoderm, suggesting their equivalence to a later stage of embryogenesis than other hEC and hES cells.

An Assessment of Hydrology, Fluvial Geomorphology, and Stream Ecology in the Cardwell Branch Watershed, Nebraska, 2003-04

USGS Publications Warehouse

Rus, David L.; Dietsch, Benjamin J.; Woodward, Brenda K.; Fry, Beth E.; Wilson, Richard C.

2007-01-01

An assessment of the 16.3-square-mile Cardwell Branch watershed characterized the hydrology, fluvial geomorphology, and stream ecology in 2003-04. The study - performed by the U.S. Geological Survey in cooperation with the City of Lincoln, Nebraska, and the Lower Platte South Natural Resources District - focused on the 7.7-square-mile drainage downstream from Yankee Hill Reservoir. Hydrologic and hydraulic models were developed using the Hydrologic Modeling System (HEC-HMS) and River Analysis System (HEC-RAS) of the U.S. Army Corps of Engineers Hydraulic Engineering Center. Estimates of streamflow and water-surface elevation were simulated for 24-hour-duration design rainstorms ranging from a 50-percent frequency to a 0.2-percent frequency. An initial HEC-HMS model was developed using the standardized parameter estimation techniques associated with the Soil Conservation Service curve number technique. An adjusted HEC-HMS model also was developed in which parameters were adjusted in order for the model output to better correspond to peak streamflows estimated from regional regression equations. Comparisons of peak streamflow from the two HEC-HMS models indicate that the initial HEC-HMS model may better agree with the regional regression equations for higher frequency storms, and the adjusted HEC-HMS model may perform more closely to regional regression equations for larger, rarer events. However, a lack of observed streamflow data, coupled with conflicting results from regional regression equations and local high-water marks, introduced considerable uncertainty into the model simulations. Using the HEC-RAS model to estimate water-surface elevations associated with the peak streamflow, the adjusted HEC-HMS model produced average increases in water-surface elevation of 0.2, 1.1, and 1.4 feet for the 50-, 1-, and 0.2-percent-frequency rainstorms, respectively, when compared to the initial HEC-HMS model. Cross-sectional surveys and field assessments conducted between November 2003 and March 2004 indicated that Cardwell Branch and its unnamed tributary appear to be undergoing incision (the process of downcutting) (with three locations showing 2 or more feet of streambed incision since 1978) that is somewhat moderated by the presence of grade controls and vegetation along the channel profile. Although streambank failures were commonly observed, 96 percent of the surveyed cross sections were classified as stable by planar and rotational failure analysis-a disconnect that may have been the result of assumed soil properties. Two process-based classification systems each indicated that the reaches within the study area were incising and widening, and the Rosgen classification system characterized the streams as either type E6 or B6c. E6 channels are hydraulically efficient with low width-depth ratios, low to moderate sinuosity, and gentle to moderately steep slopes. B6c channels typically are incised with low width-depth ratios maintained by riparian vegetation, low bedload transport, and high washload transport. No obvious nickpoints (interruption or break in slope) were observed in the thalweg profile (line of maximum streambed descent), and the most acute incision occurred immediately downstream from bridges and culverts. Nine water-quality samples were collected between August 2003 and November 2004 near the mouth of the watershed. Sediment-laden rainfall-runoff substantially affected the water quality in Cardwell Branch, leading to greater biochemical and chemical oxygen demands as well as increased concentrations of several nutrient, bacteriological, sediment, and pesticide constituents. The storage of rainfall runoff in Yankee Hill Reservoir may prolong the presence of runoff-related constituents downstream. Across the study area, there was a lack of habitat availability for aquatic biota because of low dissolved oxygen levels and low streamflows or dry channels. In August 2003, the aquatic community near the mouth of

Occurrence of virulence-associated genes in Arcobacter butzleri and Arcobacter cryaerophilus isolates from foodstuff, water, and clinical samples within the Czech Republic.

PubMed

Šilha, David; Vacková, Barbora; Šilhová, Lucie

2018-06-24

Bacteria of the Arcobacter (A.) genus, originating mainly from food and water, are dreaded germs for humans as well as animals. However, the virulence of these bacteria has not been fully elucidated yet. This study looked at the occurrence of eight virulence-associated factors (ciaB, cj1349, pldA, irgA, hecA, tlyA, mviN, hecB) in a total of 80 isolates of Arcobacter butzleri and 22 isolates of A. cryaerophilus. The isolates were derived from food, water, and clinical samples. A polymerase chain reaction using specific primers was used to detect these virulence-associated genes. The presence of all genes in the isolates of A. butzleri (98.8% ciaB, 95.0% cj1349, 98.8% pldA, 22.5% irgA, 31.3% hecA, 95.0% tlyA, 97.5% mviN, 38.8% hecB) and A. cryaerophilus (95.5% ciaB, 0.0% cj1349, 9.1% pldA, 0.0% irgA, 0.0% hecA, 31.8% tlyA, 90.9% mviN, 0.0% hecB) was monitored. Among the tested isolates, there were 13 isolates (12.7%) of A. butzleri, in which the presence of all eight virulence-associated genes was recorded in the genome. In contrast, in one A. cryaerophilus strain, none of the observed genes were detected. The presence of ciaB and mviN genes was significantly more frequent in A. cryaerophilus isolates than other genes (P < 0.05). In general, more virulence-associated genes have been detected in A. butzleri isolates compared to A. cryaerophilus. The most common gene combination (ciaB, cj1349, pldA, tlyA, mviN) was detected in case of 39 isolates. In 50.0% of A. butzleri isolates derived from clinical samples, all eight virulence-associated genes were significantly more frequently detected (P < 0.05). The tlyA gene occurred significantly more frequent in A. butzleri isolates from meat and water samples and irgA and hecB genes in clinical samples. Therefore, our study provides information about occurrence of virulence-associated genes in genome of Arcobacter isolates. These findings could be hazardous to human health, because the presence of virulence-associated genes is the assumption for potential dangerousness of these bacteria. Our results indicate high incidence of virulence-associated genes in Arcobacter genomes and hence potentially pathogenic properties of the studied strains.

Getting Its Ducks in a Row? Qatar Foundation's Agreement with HEC Paris and the Launch of the Qatar Foundation Management, Education and Research Centre

ERIC Educational Resources Information Center

Observatory on Borderless Higher Education, 2010

2010-01-01

Last week, private, non-profit organisation the Qatar Foundation (QF) announced a partnership agreement with French business institution HEC Paris to offer executive education programmes. According to the terms of the agreement, a full-time faculty will provide executive and short certificate programmes and corporate-specific training, including…

Characterization of the biological activity of a potent small molecule Hec1 inhibitor TAI-1

PubMed Central

2014-01-01

Background Hec1 (NDC80) is an integral part of the kinetochore and is overexpressed in a variety of human cancers, making it an attractive molecular target for the design of novel anticancer therapeutics. A highly potent first-in-class compound targeting Hec1, TAI-1, was identified and is characterized in this study to determine its potential as an anticancer agent for clinical utility. Methods The in vitro potency, cancer cell specificity, synergy activity, and markers for response of TAI-1 were evaluated with cell lines. Mechanism of action was confirmed with western blotting and immunofluorescent staining. The in vivo potency of TAI-1 was evaluated in three xenograft models in mice. Preliminary toxicity was evaluated in mice. Specificity to the target was tested with a kinase panel. Cardiac safety was evaluated with hERG assay. Clinical correlation was performed with human gene database. Results TAI-1 showed strong potency across a broad spectrum of tumor cells. TAI-1 disrupted Hec1-Nek2 protein interaction, led to Nek2 degradation, induced significant chromosomal misalignment in metaphase, and induced apoptotic cell death. TAI-1 was effective orally in in vivo animal models of triple negative breast cancer, colon cancer and liver cancer. Preliminary toxicity shows no effect on the body weights, organ weights, and blood indices at efficacious doses. TAI-1 shows high specificity to cancer cells and to target and had no effect on the cardiac channel hERG. TAI-1 is synergistic with doxorubicin, topotecan and paclitaxel in leukemia, breast and liver cancer cells. Sensitivity to TAI-1 was associated with the status of RB and P53 gene. Knockdown of RB and P53 in cancer cells increased sensitivity to TAI-1. Hec1-overexpressing molecular subtypes of human lung cancer were identified. Conclusions The excellent potency, safety and synergistic profiles of this potent first-in-class Hec1-targeted small molecule TAI-1 show its potential for clinically utility in anti-cancer treatment regimens. PMID:24401611

G protein-coupled estrogen receptor (GPER) expression in endometrial adenocarcinoma and effect of agonist G-1 on growth of endometrial adenocarcinoma cell lines.

PubMed

Skrzypczak, Maciej; Schüler, Susanne; Lattrich, Claus; Ignatov, Atanas; Ortmann, Olaf; Treeck, Oliver

2013-11-01

The G protein-coupled estrogen receptor (GPER, GPR30) is suggested to be involved in non-nuclear estrogen signaling and is expressed in a variety of hormone dependent cancer entities. This study was performed to further elucidate the role of this receptor in endometrial adenocarcinoma. We first analyzed GPER expression at the mRNA level in 88 endometrial cancer or normal endometrial tissue samples and compared it to those of nuclear steroid hormone receptors. GPER transcript levels were found to be about 6-fold reduced, but still present in endometrial cancer. Expression of this receptor was decreased in all grading subgroups when compared to pre- or postmenopausal endometrium. GPER mRNA expression was associated with PR mRNA levels (Spearman's rho 0.4610, p<0.001). We then tested the effect of the GPER ligand G-1 on growth of three endometrial cancer cell lines with different GPER expression. GPER protein levels were highest in RL95-2 cells, moderate in HEC-1A cells and not detectable in HEC-1B cells. The moderate expression level in HEC-1A cells was similar to average tumor tissue expression. Treatment with G-1 significantly inhibited growth of the GPER-positive cell lines RL95-2 and HEC-1A in a dose-dependent manner, whereas the GPER-negative line HEC-1B was not affected. Though GPER transcript levels were found to be reduced in endometrial cancer, our in vitro data suggest that moderate GPER expression might be sufficient to mediate growth-inhibitory effects triggered by its agonist G-1. Copyright © 2013 Elsevier Inc. All rights reserved.

Advanced airway management in hoist and longline operations in mountain HEMS - considerations in austere environments: a narrative review This review is endorsed by the International Commission for Mountain Emergency Medicine (ICAR MEDCOM).

PubMed

Pietsch, Urs; Knapp, Jürgen; Kreuzer, Oliver; Ney, Ludwig; Strapazzon, Giacomo; Lischke, Volker; Albrecht, Roland; Phillips, Patrick; Rauch, Simon

2018-04-03

Providing sufficient oxygenation and ventilation is of paramount importance for the survival of emergency patients. Therefore, advanced airway management is one of the core tasks for every rescue team. Endotracheal intubation is the gold standard to secure the airway in the prehospital setting. This review aims to highlight special considerations for advanced airway management preceding human external cargo (HEC) evacuations. We systematically searched MEDLINE, EMBASE, and PubMed in August 2017 for articles on airway management and ventilation in patients before hoist or longline operation in HEMS. Relevant reference lists were hand-searched. Three articles with regard to advanced airway management and five articles concerning the epidemiology of advanced airway management in hoist or longline rescue missions were included. We found one case report regarding ventilation during hoist operations. The exact incidence of advanced airway management before evacuation of a patient by HEC is unknown but seems to be very low (< 5%). There are several hazards which can impede mechanical ventilation of patients during HEC extractions: loss of equipment, hyperventilation, inability to ventilate and consequent hypoxia, as well as inadequacy of monitoring. Advanced airway management prior to HEC operation is rarely performed. If intubation before helicopter hoist operations (HHO) and human cargo sling (HCS) extraction is considered by the rescue team, a risk/benefit analysis should be performed and a clear standard operating procedure (SOP) should be defined. Continuous and rigorous training including the whole crew is required. An international registry on airway management during HEC extraction would be desirable.

Efficacy and safety of rolapitant for prevention of chemotherapy-induced nausea and vomiting over multiple cycles of moderately or highly emetogenic chemotherapy.

PubMed

Rapoport, Bernardo; Schwartzberg, Lee; Chasen, Martin; Powers, Dan; Arora, Sujata; Navari, Rudolph; Schnadig, Ian

2016-04-01

Rolapitant, a novel neurokinin-1 receptor antagonist (RA), was shown to protect against delayed chemotherapy-induced nausea and vomiting (CINV) during the first cycle of moderately emetogenic chemotherapy (MEC) or highly emetogenic chemotherapy (HEC) in randomized, double-blind trials. This analysis explored the efficacy and safety of rolapitant in preventing CINV over multiple cycles of MEC or HEC. Patients in one phase III MEC, one phase II HEC, and two phase III HEC clinical trials were randomized to receive oral rolapitant (180 mg) or placebo in combination with a 5-hydroxytryptamine type 3 RA and dexamethasone. Regardless of response in cycle 1, patients could continue the same antiemetic treatment for up to six cycles. On days 6-8 of each subsequent chemotherapy cycle, patients reported the incidence of emesis and/or nausea interfering with normal daily life. Post hoc analyses of pooled safety and efficacy data from the four trials were performed for cycles 2-6. Significantly more patients receiving rolapitant than control reported no emesis or interfering nausea (combined measure) in cycles 2 (p = 0.006), 3 (p < 0.001), 4 (p = 0.001), and 5 (p = 0.021). Over cycles 1-6, time-to-first emesis was significantly longer with rolapitant than with control (p < 0.001). The incidence of treatment-related adverse events during cycles 2-6 was similar in rolapitant (5.5%) and control (6.8%) arms. No cumulative toxicity was observed. Over multiple cycles of MEC or HEC, rolapitant provided superior CINV protection and reduced emesis and nausea interfering with daily life compared with control and remained well tolerated. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

Antiemetic therapy in Asia Pacific countries for patients receiving moderately and highly emetogenic chemotherapy--a descriptive analysis of practice patterns, antiemetic quality of care, and use of antiemetic guidelines.

PubMed

Yu, Shiying; Burke, Thomas A; Chan, Alexandre; Kim, Hoon-Kyo; Hsieh, Ruey Kuen; Hu, Xichun; Liang, Jin-Tung; Baños, Ana; Spiteri, Carmel; Keefe, Dorothy M K

2015-01-01

This paper reports prescribing patterns for prophylaxis of chemotherapy-induced nausea and vomiting (CINV) after highly or moderately emetogenic chemotherapy (HEC or MEC) for cancer in six Asia Pacific countries. In a prospective noninterventional study, 31 sites in Australia, China, India, Singapore, South Korea, and Taiwan recorded details of CINV prophylaxis for the acute phase (first 24 h) and delayed phase (days 2-5) after single-day HEC or MEC for adult patients. Additional information on CINV prophylactic medications was collected from 6-day patient diaries. Primary antiemetic therapies were defined as corticosteroids, the 5-hydroxytryptamine-3 receptor antagonists (5HT3-RAs), and neurokinin-1 receptor antagonists (NK1-RAs). Evaluable patients in cycle 1 numbered 648 (318 [49%] HEC and 330 [51%] MEC) of mean (SD) age of 56 (12) years, including 58% women. For the acute phase after HEC, overall (and country range), 96% (91-100%) of patients received a 5HT3-RA, 87% (70-100%) a corticosteroid, and 43% (0-91%) an NK1-RA. CINV prophylaxis for the HEC delayed phase was more variable: including 22% (7-65%) 5HT3-RA, 52% (12-93%) corticosteroid, and 46% (0-88%) NK1-RA. For the MEC acute phase, 97% (87-100%) of patients received 5HT3-RA and 86% (73-97%) a corticosteroid. For the MEC delayed phase, 201 patients (61%) received a primary antiemetic, including 5HT3-RA (41%), corticosteroid (37%), and/or NK1-RA (4%). The 5HT3-RAs were prescribed consistently in all countries, while prescribing of other antiemetic therapies was variable, and corticosteroids were under-prescribed for CINV prophylaxis, particularly in the delayed phase.

Prevention of chemotherapy-induced nausea: the role of neurokinin-1 (NK1) receptor antagonists.

PubMed

Bošnjak, Snežana M; Gralla, Richard J; Schwartzberg, Lee

2017-05-01

Chemotherapy-induced nausea (CIN) has a significant negative impact on the quality of life of cancer patients. The use of 5-hydroxytryptamine-3 (5-HT 3 ) receptor antagonists (RAs) has reduced the risk of vomiting, but (except for palonosetron) their effect on nausea, especially delayed nausea, is limited. This article reviews the role of NK 1 RAs when combined with 5-HT 3 RA-dexamethasone in CIN prophylaxis. Aprepitant has not shown consistent superiority over a two-drug (ondansetron-dexamethasone) combination in nausea control after cisplatin- or anthracycline-cyclophosphamide (AC)-based highly emetogenic chemotherapy (HEC). Recently, dexamethasone and dexamethasone-metoclopramide were demonstrated to be non-inferior to aprepitant and aprepitant-dexamethasone, respectively, for the control of delayed nausea after HEC (AC/cisplatin), and are now recognized in the guidelines. The potential impact of the new NK 1 RAs rolapitant and netupitant (oral fixed combination with palonosetron, as NEPA) in CIN prophylaxis is discussed. While the clinical significance of the effect on nausea of the rolapitant-granisetron-dexamethasone combination after cisplatin is not conclusive, rolapitant addition showed no improvement in nausea prophylaxis after AC or moderately emetogenic chemotherapy (MEC). NEPA was superior to palonosetron in the control of nausea after HEC (AC/cisplatin). Moreover, the efficacy of NEPA in nausea control was maintained over multiple cycles of HEC/MEC. Recently, NK 1 RAs have been challenged by olanzapine, with olanzapine showing superior efficacy in nausea prevention after HEC. Fixed antiemetic combinations (such as NEPA) or new antiemetics with a long half-life that may be given once per chemotherapy cycle (rolapitant or NEPA) may improve patient compliance with antiemetic treatment.

Role of High-End Computing in Meeting NASA's Science and Engineering Challenges

NASA Technical Reports Server (NTRS)

Biswas, Rupak; Tu, Eugene L.; Van Dalsem, William R.

2006-01-01

Two years ago, NASA was on the verge of dramatically increasing its HEC capability and capacity. With the 10,240-processor supercomputer, Columbia, now in production for 18 months, HEC has an even greater impact within the Agency and extending to partner institutions. Advanced science and engineering simulations in space exploration, shuttle operations, Earth sciences, and fundamental aeronautics research are occurring on Columbia, demonstrating its ability to accelerate NASA s exploration vision. This talk describes how the integrated production environment fostered at the NASA Advanced Supercomputing (NAS) facility at Ames Research Center is accelerating scientific discovery, achieving parametric analyses of multiple scenarios, and enhancing safety for NASA missions. We focus on Columbia s impact on two key engineering and science disciplines: Aerospace, and Climate. We also discuss future mission challenges and plans for NASA s next-generation HEC environment.

Physical Model Study of Cross Vanes and Ice

DTIC Science & Technology

2009-08-01

spacing since, in the pre-scour state, experiments and the HEC - RAS hydraulic model (USACE 2002b) found that water surface ele- vation merged with the...docs/eng-manuals/em1110- 2-1612/toc.htm. USACE (2002b) HEC - RAS , Hydraulic Reference Manual. US Army Corps of Engineers Hydrologic Engineering Center...Currently little design guidance is available for constructing these structures on ice-affected rivers . This study used physical and numerical

Hydroxyethyl cellulose doped with copper(II) phthalocyanine-tetrasulfonic acid tetrasodium salt as an effective dual functional hole-blocking layer for polymer light-emitting diodes

NASA Astrophysics Data System (ADS)

Wu, Cheng-Liang; Chen, Yun

2017-07-01

We report a doping method to improve the performance of solution-processed polymer light-emitting diodes (PLEDs). Doping 12 wt% copper(II) phthalocyanine-tetrasulfonated acid tetrasodium salt (TS-CuPc) into hydroxyethyl cellulose (HEC) as a dual functional hole-blocking layer (df-HBL) of multilayer PLED (glass/ITO/PEDOT:PSS/HY-PPV/TS-CuPc-doped HEC/LiF/Al) significantly enhanced maximum luminance, maximum current and power efficiency over that without the df-HBL (10,319 cd/m2, 2.98 cd/A and 1.24 lm/W) to (29,205 cd/m2, 13.27 cd/A and 9.56 lm/W). CV measurements reveal that HEC possesses a powerful hole-blocking capability. Topography and conductivity AFM images show that doping TS-CuPc increases the interfacial contact area and interfacial conductivity, which can overcome the insulating nature of HEC and thus further facilitate electron injection. Enhancements in device performance are attributed to the improved carrier balance and recombination in the presence of df-HBL, confirmed in electron-only and hole-only devices. Moreover, apparently raised open-circuit voltages provide further evidence that enhanced electron injection is indeed realized by the df-HBL. This study demonstrates an effective approach to develop highly efficient PLEDs.

Ectopic expression of plasma membrane targeted subunits of the Ndc80-complex as a tool to study kinetochore biochemistry.

PubMed

Holmström, Tim H; Rehnberg, Jonathan; Ahonen, Leena J; Kallio, Marko J

2009-06-01

Genomic stability depends on the normal function of the kinetochore, a multi-protein assemblage, which consists of over 80 molecules including both constitutive and transiently binding components. Information regarding the spatial-temporal assembly of kinetochore subcomplexes is often limited by technical difficulties in their isolation. To study kinetochore subcomplex formation, we targeted separately Hec1 and Spc24, two subunits of the Ndc80 kinetochore compilation, to the plasma membrane by fusing them with the amino-terminal palmitoylation and myristoylation (pm) sequence of the receptor tyrosine kinase Fyn. We found that in early mitotic cells, pm-GFP-Hec1 and pm-GFP-Spc24 fusion proteins localised to the plasma membrane and were able to recruit all subunits of the Ndc80 complex (Ndc80/Hec1, Nuf2, Spc24 and Spc25) to these foci. In interphase cells, only Hec1-Nuf2 and Spc24-Spc25 heterodimers accumulated to the plasma membrane foci. The results propose that the assembly of Ndc80 tetramer can take place outside of the kinetochore but requires co-factors that are only present in mitotic cells. These findings provide the first experimental evidence on the successful employment of the plasma membrane targeting technique in the study of kinetochore biochemistry.

Observed and Predicted Pier Scour in Maine

USGS Publications Warehouse

Hodgkins, Glenn A.; Lombard, Pamela J.

2002-01-01

Pier-scour and related data were collected and analyzed for nine high river flows at eight bridges across Maine from 1997 through 2001. Six bridges had multiple piers. Fifteen of 23 piers where data were measured during a high flow had observed maximum scour depths ranging from 0.5 feet (ft) to 12.0 ft. No pier scour was observed at the remaining eight piers. The maximum predicted pier-scour depths associated with the 23 piers were computed using the equations in the Federal Highway Administration's Hydraulic Engineering Circular number 18 (HEC-18), with data collected for this study. The predicted HEC-18 maximum pier-scour depths were compared to the observed maximum pier-scour depths. The HEC-18 pier-scour equations are intended to be envelope equations, ideally never underpredicting scour depths and not appreciably overpredicting them. The HEC-18 pier-scour equations performed well for rivers in Maine. Twenty-two out of 23 pier-scour depths were overpredicted by 0.7 ft to 18.3 ft. One pier-scour depth was underpredicted by 4.5 ft. For one pier at each of two bridges, large amounts of debris lodged on the piers after high-flow measurements were made at those sites. The scour associated with the debris increased the maximum pier-scour depths by about 5 ft in each case.

“No-o-o-o Peeking”: Preschoolers’ Executive Control, Social Competence, and Classroom Adjustment

PubMed Central

Denham, Susanne A.; Bassett, Hideko H.; Sirotkin, Yana S.; Brown, Chavaughn; Morris, Carol S.

2015-01-01

The goals of this study were to evaluate (1) how specific aspects of executive control, briefly assessed, predict social competence and classroom adjustment during preschool; and (2) differences between two aspects of executive control, according to child’s age, socioeconomic risk status, and gender. The facets of executive control were defined as cool executive control (CEC; affectively neutral, slow acting, and late developing) and hot executive control (HEC; more emotional, fast acting, and early developing). Two hundred eighty-seven 3- to 5-year-old children from private child care and Head Start centers were directly assessed during executive control tasks, and preschool teachers provided information on their school success. Aspects of executive control varied with age, socioeconomic risk, and gender. Specifically, older children performed better on CEC tasks across three age levels; for HEC tasks, change was seen only between 3-year-olds and 4-year-olds. Children of mothers with less formal education performed less well on CEC than those whose mothers had more education; girls performed better than boys on HEC tasks. Further, facets of executive control were differentially related to later social competence and classroom adjustment. HEC predicted social competence, whereas CEC uniquely predicted classroom adjustment. Implications for everyday practice and specific curricula formulation are discussed. PMID:26166925

[The Role of 5-Aza-CdR on Methylation of Promoter in RASSF1A Gene in Endometrial Carcinoma].

PubMed

Huang, Li-ping; Chen, Chen; Wang, Xue-ping; Liu, Hui

2015-05-01

To explore the effect of demethylating drug 5-Aza-2'-deoxycytidine (5-Aza-CdR) on methtylation status of the Ras-association domain familylA gene (RASSF1A) in human endometrial carcinoma. Randomly'assign the human endometrial carcinoma cell line HEC-1-B into groups and use demethylating drug 5-Aza-CdR of different concentration to treat them. Then Methylation-specific polymerase chain reaction (MSP), real-time PCR, Western blot, TUNEL technology were used to analyze methylation status of RASSF1A promoter CpG islands, RASSF1A mRNA expression, RASSF1A protein expression and apoptosis of HEC-1-B cell. High DNA methylation in RASSF1A gene promoter region, low RASSF1A mRNA level and protein expression and out of control of human endometrial carcinoma cell HEC-1-B apoptosis were observed. 5-Aza-CdR of different concentration could reverse RASSF1A gene's methylation status, recover the expression of mRNA and protein, and control the growth of HEC-1-B by inducing apoptosis. Aberrant methylation of RASSF1A in endometrial cancer as a therapeutic target, demethylating agent 5-Aza-CdR could be an effective way of gene therapy.

Theoretical investigation of optical properties and band gap engineering for Zn1-x TM xTe (TM = Fe, Co) alloys by modified Becke-Johnson potential

NASA Astrophysics Data System (ADS)

Mahmood, Q.; Yaseen, M.; Hassan, M.; Ramay, Shahid M.; Mahmood, Asif

2017-08-01

Not Available Project supported by the Deanship of Scientific Research at King Saud University (for Shahid M Ramay) (Grant No. RG 1435-004), the University of the Punjab, Lahore for financial support through Faculty Research Grant Program (for M Hassan), and the HEC Pakistan (Grant No. 21-261/SRGP/R&D/HEC/2014) (for M Yaseen).

Potential Improvements for HEC-HMS Automated Parameter Estimation

DTIC Science & Technology

2006-08-01

and is now a graduate student in the Department of Civil and Environmental Engineering at the University of Illinois at Urbana /Champaign. Daniel...divided into 14 nested subwatersheds with a flow measuring flume constructed at each of the subwatershed outlets. The drainage areas above the...boundaries and stream network, and rain and stream gauge locations are shown in Figure 1. The first HEC-HMS model was applied to the 39.8-acre drainage

Activated carbons derived from coconut shells as high energy density cathode material for Li-ion capacitors

PubMed Central

Jain, Akshay; Aravindan, Vanchiappan; Jayaraman, Sundaramurthy; Kumar, Palaniswamy Suresh; Balasubramanian, Rajasekhar; Ramakrishna, Seeram; Madhavi, Srinivasan; Srinivasan, M. P.

2013-01-01

In this manuscript, a dramatic increase in the energy density of ~ 69 Wh kg−1 and an extraordinary cycleability ~ 2000 cycles of the Li-ion hybrid electrochemical capacitors (Li-HEC) is achieved by employing tailored activated carbon (AC) of ~ 60% mesoporosity derived from coconut shells (CS). The AC is obtained by both physical and chemical hydrothermal carbonization activation process, and compared to the commercial AC powders (CAC) in terms of the supercapacitance performance in single electrode configuration vs. Li. The Li-HEC is fabricated with commercially available Li4Ti5O12 anode and the coconut shell derived AC as cathode in non-aqueous medium. The present research provides a new routine for the development of high energy density Li-HEC that employs a mesoporous carbonaceous electrode derived from bio-mass precursors. PMID:24141527

APF530 (granisetron injection extended-release) in a three-drug regimen for delayed CINV in highly emetogenic chemotherapy.

PubMed

Schnadig, Ian D; Agajanian, Richy; Dakhil, Christopher; Gabrail, Nashat Y; Smith, Robert E; Taylor, Charles; Wilks, Sharon T; Schwartzberg, Lee S; Cooper, William; Mosier, Michael C; Payne, J Yvette; Klepper, Michael J; Vacirca, Jeffrey L

2016-06-01

APF530, extended-release granisetron, provides sustained release for ≥5 days for acute- and delayed-phase chemotherapy-induced nausea and vomiting (CINV). We compared efficacy and safety of APF530 versus ondansetron for delayed CINV after highly emetogenic chemotherapy (HEC), following a guideline-recommended three-drug regimen. HEC patients received APF530 500 mg subcutaneously or ondansetron 0.15 mg/kg intravenously, with dexamethasone and fosaprepitant. Primary end point was delayed-phase complete response (no emesis or rescue medication). A higher percentage of APF530 versus ondansetron patients had delayed-phase complete response (p = 0.014). APF530 was generally well tolerated; treatment-emergent adverse event incidence was similar across arms, mostly mild-to-moderate injection-site reactions. APF530 versus the standard three-drug regimen provided superior control of delayed-phase CINV following HEC. ClinicalTrials.gov : NCT02106494.

Opening the black box of ethics policy work: evaluating a covert practice.

PubMed

Frolic, Andrea; Drolet, Katherine; Bryanton, Kim; Caron, Carole; Cupido, Cynthia; Flaherty, Barb; Fung, Sylvia; McCall, Lori

2012-01-01

Hospital ethics committees (HECs) and ethicists generally describe themselves as engaged in four domains of practice: case consultation, research, education, and policy work. Despite the increasing attention to quality indicators, practice standards, and evaluation methods for the other domains, comparatively little is known or published about the policy work of HECs or ethicists. This article attempts to open the "black box" of this health care ethics practice by providing two detailed case examples of ethics policy reviews. We also describe the development and application of an evaluation strategy to assess the quality of ethics policy review work, and to enable continuous improvement of ethics policy review processes. Given the potential for policy work to impact entire patient populations and organizational systems, it is imperative that HECs and ethicists develop clearer roles, responsibilities, procedural standards, and evaluation methods to ensure the delivery of consistent, relevant, and high-quality ethics policy reviews.

[Regulation of [12Asp]K-ras4B on transcriptional activity of estrogen receptor in endometrial carcinoma HEC-1A cell lines].

PubMed

Gui, Li-ming; Wei, Li-hui; Xu, Ming-xu; Wang, Jian-liu; Zhong, Ying-cheng; Li, Xiao-ping; Tu, Zheng; Sun, Peng-ming; Ma, Da-long

2004-01-01

To investigate the effect of mutant-type [(12)Asp]K-ras4B gene on the expression of estrogen receptor (ER) alpha and beta and their transcriptional activity as a transcription factor in endometrial carcinoma HEC-1A cell line. (1) Effect of [(12)Asp]K-ras4B on the expression of ER alpha and beta were determined using Western blot assay. (2) Eukaryotic expression plasmid pGL3-luciferase-ERE containing luciferase report gene and estrogen receptor element (ERE) was constructed, and co-transfected into NIH3T3 and HEC-1A cell lines with pEGFP-N1 to examine the effect of [(12)Asp]K-ras4B on ER transcription that is regulated by estradiol. In addition, they were transfected into pSV5-HER0 (containing full length wide type ERalpha cDNA) and pCMV-rafS621A (inhibiting raf kinase) plasmids to test the effect of [(12)Asp]K-ras4B/raf signal pathway on transcriptional activity of ER proteins. (1) Protein level of ERs expressed in pcDI transfected control cells was low while it was increased for 3.6-fold (97 +/- 25, 349 +/- 67, P < 0.01) and 1.9-fold (128 +/- 37, 349 +/- 30, P < 0.05) in ERalpha and ERbeta, respectively, in pcDI-[(12)Asp]K-ras4B NIH3T3 cells after transfection. (2) In pcDI-[(12)Asp]K-ras4B NIH3T3 cells, the ratios for ERalpha and and ERbeta levels before transfection of rafS621A plasmids to that after the transfection, were 2.4:1 (724 +/- 45, 310 +/- 46, P < 0.05) and 1.8:1 (493 +/- 20, 284 +/- 20, P < 0.01), respectively; In HEC-1A cells, these ratios were 2.1:1 (566 +/- 22, 279 +/- 30, P < 0.01) and 2.4:1 (405 +/- 33, 165 +/- 15, P < 0.01), respectively. (3) In low serum (2%) culture condition, estradiol (E(2)) stimulated luciferase activity with an increase of 13-fold (130 +/- 42, 1681 +/- 242, P < 0.01) in pcDI-[(12)Asp] K-ras4B NIH3T3 cells, 19-fold (141 +/- 39, 2644 +/- 331, P < 0.001) in HEC-1A cells, respectively, when compared with those in the absence of E(2). (4) In pSV5-HER0 transfected pcDI-[(12)Asp] K-ras4B NIH3T3 cells and HEC-1A cells, compared to the untransfected cells, the ER transcriptional activity in the transfected cells increased markedly. The luciferase activity was increased for 8-fold (1048 +/- 91, 8099 +/- 452, P < 0.01) and 6-fold (2148 +/- 259, 12,705 +/- 2670, P < 0.001), respectively. rafS621A mutant had suppressive effects on luciferase activities in HEC-1A cells and pcDI-[(12)Asp]K-ras4B NIH3T3 cells. The ratio of luciferase activities in pcDI-[(12)Asp]K-ras4B NIH3T3 and HEC-1A cells, before and after transfection was 7.8:1 (1184 +/- 168, 152 +/- 27, P < 0.05) and 6.4:1 (1949 +/- 212, 304 +/- 60, P < 0.01), respectively. (1) [(12)Asp]K-ras4B can enhance the expression of ERalpha and beta proteins. This may be correlated with [(12)Asp]K-ras4B/raf signaling pathway. (2) The effect of mutant-type [(12)Asp]K-ras4B gene on ERs transcriptional activity in HEC-1A cells appears to need E(2).

A Study on Estimation on Flood Warning Trigger Rainfall in medium and small Stream Affected by Urban Effects

NASA Astrophysics Data System (ADS)

Youngseok, Song; Moojong, Park; JungHo, Lee; HeeSup, Lee

2013-04-01

As extreme floods occur frequently in recent years due to global climate changes, an in sudden local flooding of great volume and short duration is becoming the significant danger and loss of life and property in the Korean Peninsula as well as most parts of the world. The desire for living without hazardous damages grows these days, the city strategy to make the safer community has become an issue. Previously most of flood prevention efforts have been made for relatively large watersheds near to channel flow. However, as economical development and the expansion of city near medium and small stream, human casualty and property by flood occurs frequently. Therefore, to reduce the damage of human lives and property by flood, we develop an assessment method for flood warning trigger rainfall considering urban effect. Considering complex land use, HEC-HMS is used for rural area and SWMM is adopted for sewer networks runoff. And relationship between runoff and stream water level, HEC-RAS is accompanied with runoff results. Proposed flood warning trigger rainfall assessment method shows good agreement with gauged data and could be used for another case to mitigate damage. Acknowledgement: "This research was supported by a grant [NEMA-NH-2011-45] from the Natural Hazard Mitigation Research Group, National Emergency Management Agency of Korea." Keyword: HEC-HMS, HEC-RAS, critical precipitation, medium and small stream

Effect of mTOR Inhibitors in Nude Mice with Endometrial Carcinoma and Variable PTEN Expression Status

PubMed Central

Fong, Pedro; Meng, Li-rong

2014-01-01

Background The aim of this study was to investigate the sensitivity to rapamycin of endometrial cancer cells with different phosphatase and tensin homologue (PTEN) expression to understand the mechanism of resistance to mammalian target of rapamycin (mTOR) inhibitors in the treatment of endometrial cancer. Material/Methods Twenty specific pathogen-free female BALB/c mice received transplants of either HEC-1A (PTEN-positive) or Ishikawa (PTEN-negative) cells. Mice in the treatment group were injected intraperitoneally once a week for 4 consecutive weeks. The control group was injected weekly with phosphate buffer saline (PBS) for 4 consecutive weeks. Tumor volume, tumor mass, growth curves, and inhibition rate were measured, after which the mice were killed. Results Both tumor growth rate and size were slower in the treatment group than in the control group for all mice that received transplants of either HEC-1A or Ishikawa cells. The tumor inhibition rates in the treatment group were 48.1% and 67.1% in mice transplanted with HEC-1A and Ishikawa cells, respectively. Conclusions The inhibitory effects of rapamycin were enhanced in PTEN-negative Ishikawa tumor cells compared with PTEN-positive HEC-1A cells, which could explain the reduced effect of rapalogues in some endometrial cancer patients and help to understand the mechanism of resistance to this drug. PMID:25266877

Applying 1D Sediment Models to Reservoir Flushing Studies: Measuring, Monitoring, and Modeling the Spencer Dam Sediment Flush with HEC-RAS

DTIC Science & Technology

2016-07-01

approximately 5 hours (hr) after opening main gates. Multiple channels eroded (Figure 5), moving sediment through the dam throughout the first day...additional sediment evacuation was observed over the next 4 weeks. ERDC/CHL CHETN-XIV-52 July 2016 5 Figure 5. Multiple channels eroded...2015. A physically-based channel - modeling framework integrating HEC-RAS sediment transport capabilities and the USDA-ARS Bank-Stability and Toe-Erosion

Hydrologic Engineering Center River Analysis System (HEC-RAS) Water Temperature Models Developed for the Missouri River Recovery Management Plan and Environmental Impact Statement

DTIC Science & Technology

2017-09-18

Temperature Models Developed for the Missouri River Recovery Management Plan and Environmental Impact Statement En vi ro nm en ta l L ab or at or y...Engineering Center-River Analysis System (HEC-RAS) Water Temperature Models Developed for the Missouri River Recovery Management Plan and Environmental...Prepared for U.S. Army Corps of Engineers Washington, DC 20314-1000 Under Project 396939, “Missouri River Recovery Management Plan and Environmental

Prototypes of Self-Powered Radiation Detectors Employing Intrinsic High-Energy Current (HEC) (POSTPRINT)

DTIC Science & Technology

2016-01-01

neutron sensi- tivities of a Pt self - powered detector ,” IEEE Trans. Nucl. Sci. 25, 292–295 (1978). 6T. A. Dellin, R. E. Huddleston, and C. J...Gamma-sensitive self - powered detectors and their use for in-core flux -mapping,” IEEE Trans. Nucl. Sci. 28, 752–757 (1981). 9E. A. Burke and J. Wall...AFCEC-CX-TY-TP-2016-0006 PROTOTYPES OF SELF - POWERED RADIATION DETECTORS EMPLOYING INTRINSIC HIGH-ENERGY CURRENT (HEC) (POSTPRINT) Piotr

Jackson Bar Training Structure Study

DTIC Science & Technology

2015-05-01

comparison of the one-dimensional bridge hydraulic routines from: HEC - RAS , HEC -2, and WSPRO. Davis, CA: U.S. Army Corps of Engineers, Hydrologic Engineering...ER D C/ CH L TR -1 5- 4 Jackson Bar Training Structure Study Co as ta l a nd H yd ra ul ic s La bo ra to ry Jeremy A. Sharp and...The hydrodynamic model was validated with gage data from the U.S. Geological Survey 02470050 Tombigbee River at Steamplant near Leroy, AL, gage

Computer Operations Study of Reservoir Operations for Six Mississippi River Headwaters Dams. Appendix A.

DTIC Science & Technology

1982-06-01

p*A C.._ _ __ _ _ A, d.tibutiou is unhimta 4 iit 84~ L0 TABLE OF CONTENTS APPENDIX SCOPE OF WORK B MERGE AND COST PROGRAM DOCUMENTATION C FATSCO... PROGRAM TO COMPUTE TIME SERIES FREQUENCY RELATIONSHIPS D HEC-DSS - TIME SERIES DATA FILE MANAGEMENT SYSTEM E PLAN 1 -TIM SERIES DATA PLOTS AND ANNUAL...University of Minnesota, utilized an early version of the Hydrologic Engineering * Center’s (HEC) EEC-5c Computer Program . EEC is a Corps of Engineers

Liquid Viscosity and Density Measurement with Flexural-Plate-Wave Sensors

DTIC Science & Technology

1996-04-01

capillary-viscometer-measured viscosity in Fig. 4. "The data from solutions of poly(ethylene glycol), having average molecular weights 3350 and 15,000...have seen similar results for the FPW-measured viscosity of salmon-sperm DNA solutions. 25 glycerol WA " PEG 3,350 H-4 . e! 2 PEG 15,000 IK- ,,,," HEC...number of aqueous solutions of the polymers poly(ethylene glycol) ( PEG ) and hydroxyethyl cellulose (HEC). The response of the FPW sensor (vertical axis

New Techniques for Real-Time Stage Forecasting for Tributaries in the Nashville Area

NASA Astrophysics Data System (ADS)

Charley, W.; Moran, B.; LaRosa, J.

2011-12-01

On Saturday, May 1, 2010, heavy rain began falling in the Cumberland River Valley, Tennessee, and continued through the following day. 13.5 inches was measured at Nashville, an unprecedented amount that doubled the previous 2-day record, and exceeded the May monthly total record of 11 inches. Elsewhere in the valley, amounts of over 19 inches were measured. This intensity of rainfall quickly overwhelmed tributaries to the Cumberland in the Nashville area, causing wide-spread and serious flooding. Tractor-trailers and houses were seen floating down Mill Creek, a primary tributary in the south eastern area of Nashville. Twenty-six people died and over 2 billion dollars in damage occurred as a result of the flood. Since that time, several other significant rainfall events have occurred in the area. As a result of the flood, agencies in the Nashville area want better capabilities to forecast stages for the local tributaries. Better stage forecasting will help local agencies close roads, evacuate homes and businesses and similar actions. An interagency group, consisting of Metro Nashville Water Services and Office of Emergency Management, the National Weather Service, the US Geological Survey and the US Army Corps of Engineers, has been established to seek ways to better forecast short-term events in the region. It should be noted that the National Weather Service has the official responsibility of forecasting stages. This paper examines techniques and algorithms that are being developed to meet this need and the practical aspects of integrating them into a usable product that can quickly and accurately forecast stages in the short-time frame of the tributaries. This includes not only the forecasting procedure, but also the procedure to acquire the latest precipitation and stage data to make the forecasts. These procedures are integrated into the program HEC-RTS, the US Army Corps of Engineers Real-Time Simulation program. HEC-RTS is a Java-based integration tool that has been derived from the Corps Water Management System (CWMS). The modeling component takes observed and forecasted rainfall to compute river flow with the program HEC-HMS. The river hydraulics program, HEC-RAS, computes river stages and water surface profiles. An inundation boundary and depth map of water in the flood plain is computed from HEC-RAS Mapper. The user-configurable sequence of modeling software allows engineers to evaluate and compare hydraulic impacts for various "what if?" scenarios. The implementation of these techniques and HEC-RTS is examined for the Mill Creek basin, the 108 square mile tributary basin south east of Nashville. Mill Creek has an average annual flow of 150 CFS and a short response time. It has suffered major damage from the 2010 and other events. The accuracy and effectiveness of the techniques in the integrated tool HEC-RTS is evaluated.

Ethiopian Central Rift Valley basin hydrologic modelling using HEC-HMS and ArcSWAT

NASA Astrophysics Data System (ADS)

Pascual-Ferrer, Jordi; Candela, Lucila; Pérez-Foguet, Agustí

2013-04-01

An Integrated Water Resources Management (IWRM) shall be applied to achieve a sustainable development, to increase population incomes without affecting lives of those who are highly dependent on the environment. First step should be to understand water dynamics at basin level, starting by modeling the basin water resources. For model implementation, a large number of data and parameters are required, but those are not always available, especially in some developing countries where different sources may have different data, there is lack of information on data collection, etc. The Ethiopian Central Rift Valley (CRV) is an endorheic basin covering an area of approximately 10,000 km2. For the period 1996-2005, the average annual volume of rainfall accounted for 9.1 Mm3, and evapotranspiration for 8 Mm3 (Jansen et al., 2007). From the environmental point of view, basin ecosystems are endangered due to human activities. Also, poverty is widespread all over the basin, with population mainly living from agriculture on a subsistence economy. Hence, there is an urgent need to set an IWRM, but datasets required for water dynamics simulation are not too reliable. In order to reduce uncertainty of numerical simulation, two semi-distributed open software hydrologic models were implemented: HEC-HMS and ArcSWAT. HEC-HMS was developed by the United States Army Corps of Engineers (USACoE) Hydrologic Engineering Center (HEC) to run precipitation-runoff simulations for a variety of applications in dendritic watershed systems. ArcSWAT includes the SWAT (Soil and Water Assessment Tool, Arnold et al., 1998) model developed for the USDA Agricultural Research Service into ArcGIS (ESRI®). SWAT was developed to assess the impact of land management practices on large complex watersheds with varying soils, land use and management conditions over long periods of time (Neitsch et al., 2005). According to this, ArcSWAT would be the best option for IWRM implementation in the basin. However, considering data uncertainty and model complexity a previous hydrologic assessment of the basin based in HEC-HMS simulation is advisable. As a first approach HEC-HMS was implemented for basin modeling in order to get physical parameters of interest, results from HEC-HMS calibration were used to setup the accuracy of the ArcSWAT numerical modelling. References Arnold, J.G., Srinivasan, R., Muttiah, R.S. & Williams, J.R. (1998). Large Area Hydrologic Modeling and Assessment Part I: Model Development. JAWRA Journal of the American Water Resources Association, Vol. 34, No. 1, pp. 73-89. Jansen, H., Hengsdijk, H., Legesse, D., Ayenew, T., Hellegers, P. & Spliethoff, P. (2007). Land and water resources assessment in the Ethiopian Central Rift Valley. In Alterra report 1587. Wageningen: Alterra. p. 81. Neitsch, S.L., Arnold, J.G., Kiniry, J.R. & Williams, J.R. (2005). Soil and Water Assessment Tool Theoretical Documentation. Version 2005, Temple, Texas.

Hydration properties of regioselectively etherified celluloses monitored by 2H and 13C solid-state MAS NMR spectroscopy.

PubMed

Larsen, Flemming H; Schöbitz, Michael; Schaller, Jens

2012-06-20

The hydration properties of 2,3-O-hydroxypropylcellulose (HPC) and 2,3-O-hydroxyethylcellulose (HEC) were analyzed by multi-nuclear solid-state MAS NMR spectroscopy. By 13C single-pulse (SP) MAS and cross-polarization (CP) MAS NMR, differences between the immobile regions and all parts of the polysaccharides were detected as a function of hydration. Complementary information about the water environments was observed by 2H MAS NMR. By this approach it was demonstrated that side chains in 2,3-O-HPC and 2,3-O-HEC were easier to hydrate than the cellulose backbone. Furthermore the motion of water was more restricted (slower) in 2,3-O-HPC than in 2,3-O-HEC. For both polysaccharides the hydration could be explained by a two-step process: in step one increased ordering of the immobile regions occurs after which the entire polymer is hydrated in step two. Copyright © 2012 Elsevier Ltd. All rights reserved.

Habitable evaporated cores: transforming mini-Neptunes into super-Earths in the habitable zones of M dwarfs.

PubMed

Luger, R; Barnes, R; Lopez, E; Fortney, J; Jackson, B; Meadows, V

2015-01-01

We show that photoevaporation of small gaseous exoplanets ("mini-Neptunes") in the habitable zones of M dwarfs can remove several Earth masses of hydrogen and helium from these planets and transform them into potentially habitable worlds. We couple X-ray/extreme ultraviolet (XUV)-driven escape, thermal evolution, tidal evolution, and orbital migration to explore the types of systems that may harbor such "habitable evaporated cores" (HECs). We find that HECs are most likely to form from planets with ∼1 M⊕ solid cores with up to about 50% H/He by mass, though whether or not a given mini-Neptune forms a HEC is highly dependent on the early XUV evolution of the host star. As terrestrial planet formation around M dwarfs by accumulation of local material is likely to form planets that are small and dry, evaporation of small migrating mini-Neptunes could be one of the dominant formation mechanisms for volatile-rich Earths around these stars.

Dosimetric properties of high energy current (HEC) detector in keV x-ray beams.

PubMed

Zygmanski, Piotr; Shrestha, Suman; Elshahat, Bassem; Karellas, Andrew; Sajo, Erno

2015-04-07

We introduce a new x-ray radiation detector. The detector employs high-energy current (HEC) formed by secondary electrons consisting predominantly of photoelectrons and Auger electrons, to directly convert x-ray energy to detector signal without externally applied power and without amplification. The HEC detector is a multilayer structure composed of thin conducting layers separated by dielectric layers with an overall thickness of less than a millimeter. It can be cut to any size and shape, formed into curvilinear surfaces, and thus can be designed for a variety of QA applications. We present basic dosimetric properties of the detector as function of x-ray energy, depth in the medium, area and aspect ratio of the detector, as well as other parameters. The prototype detectors show similar dosimetric properties to those of a thimble ionization chamber, which operates at high voltage. The initial results obtained for kilovoltage x-rays merit further research and development towards specific medical applications.

Aurora B potentiates Mps1 activation to ensure rapid checkpoint establishment at the onset of mitosis.

PubMed

Saurin, Adrian T; van der Waal, Maike S; Medema, René H; Lens, Susanne M A; Kops, Geert J P L

2011-01-01

The mitotic checkpoint prevents mitotic exit until all chromosomes are attached to spindle microtubules. Aurora B kinase indirectly invokes this checkpoint by destabilizing incorrect attachments; however, a more direct role remains controversial. In contrast, activity of the kinase Mps1 is indispensible for the mitotic checkpoint. Here we show that Aurora B and Hec1 are needed for efficient Mps1 recruitment to unattached kinetochores, allowing rapid Mps1 activation at the onset of mitosis. Live monitoring of cyclin B degradation reveals that this is essential to establish the mitotic checkpoint quickly at the start of mitosis. Delayed Mps1 activation and checkpoint establishment upon Aurora B inhibition or Hec1 depletion are rescued by tethering Mps1 to kinetochores, demonstrating that Mps1 recruitment is the primary role of Aurora B and Hec1 in mitotic checkpoint signalling. These data demonstrate a direct role for Aurora B in initiating the mitotic checkpoint rapidly at the onset of mitosis.

Palonosetron Prevents Highly Emetogenic Chemotherapy-induced Nausea and Vomiting in Oral Cancer Patients.

PubMed

Sento, Shinya; Kitamura, Naoya; Yamamoto, Tetsuya; Nakashiro, Koichi; Hamakawa, Hiroyuki; Ibaragi, Soichiro; Sasaki, Akira; Takamaru, Natsumi; Miyamoto, Yoji; Kodani, Isamu; Ryoke, Kazuo; Mishima, Katsuaki; Ueyama, Yoshiya

2017-12-01

To evaluate the efficacy of palonosetron in preventing acute and delayed nausea and vomiting in patients receiving highly emetogenic chemotherapy (HEC) in oral cancer patients. Oral cancer patients receiving HEC were enrolled; among the 40 patients, 87 courses of chemotherapy were administered. On day 1, 0.75 mg palonosetron was intravenously administrated just before chemotherapy. The primary endpoint was the proportion of patients with a complete response (CR) and the secondary endpoint was the proportion of patients with complete control (CC) during the acute and delayed phase. During the acute phase, 86 of 87 courses (98.9%) had CR and 84 of 87 courses (96.6%) had CC. During the delayed phase, 84 of 87 courses (96.6%) had CR and 70 of 87 courses (80.5%) had CC. Palonosetron is effective at preventing HEC-induced chemotherapy-induced nausea and vomiting (CINV) in oral cancer chemotherapeutic regimens in the acute and delayed phases. Copyright© 2017, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

Effect of sieving polymer concentration on separation of 100 bp DNA Ladder by capillary gel electrophoresis

NASA Astrophysics Data System (ADS)

Nakazumi, T.; Hara, Y.

2017-09-01

We studied the effect of sieving polymer concentration on separation of a 100 bp DNA Ladder by capillary gel electrophoresis (CGE) using hydroxyethyl cellulose (HEC) with a molecular size of 1000 k. For measurement purposes, we selected a fused silica capillary with total length of 15 cm and effective length of 7.5 cm; this was applied to compact CGE equipment for a Point-Care-Testing (POCT) system. Measurement results of the 100 bp DNA Ladder sample indicated that small DNA separation was significantly affected by HEC sieving polymer concentration. This was due to the level of entanglement between small DNA molecules and the sieving polymer chain significantly influencing migration time, mobility, and resolution length of the CGE process. We concluded that 1.0 w/v % HEC sieving polymer concentration was optimal for CGE separation of DNA ≥1000bp in the 100 bp DNA Ladder (100-1500 bp) when using the short-length capillary.

Spatiotemporal floodplain mapping and prediction using HEC-RAS - GIS tools: Case of the Mejerda river, Tunisia

NASA Astrophysics Data System (ADS)

Ben Khalfallah, C.; Saidi, S.

2018-06-01

The floods have become a scourge in recent years (Floods of, 2003, 2006, 2009, 2011, and 2012), increasingly frequent and devastating. Tunisia does not escape flooding problems, the flood management requires basically a better knowledge of the phenomenon (flood), and the use of predictive methods. In order to limit this risk, we became interested in hydrodynamics modeling of Medjerda basin. To reach this aim, rainfall distribution is studied and mapped using GIS tools. In addition, flood and return period estimation of rainfall are calculated using Hyfran. Also, Simulations of recent floods are calculated and mapped using HEC-RAS and HEC-GeoRAS for the most recent flood occurred in February-March 2015 in Medjerda basin. The analysis of the results shows a good correlation between simulated parameters and those measured. There is a flood of the river exceeding 240 m3/s (DGRE, 2015) and more flowing sections are observed in the future simulations; for return periods of 10yr, 20yr and 50yr.

Hydrological modelling in sandstone rocks watershed

NASA Astrophysics Data System (ADS)

Ponížilová, Iva; Unucka, Jan

2015-04-01

The contribution is focused on the modelling of surface and subsurface runoff in the Ploučnice basin. The used rainfall-runoff model is HEC-HMS comprising of the method of SCS CN curves and a recession method. The geological subsurface consisting of sandstone is characterised by reduced surface runoff and, on the contrary, it contributes to subsurface runoff. The aim of this paper is comparison of the rate of influence of sandstone on reducing surface runoff. The recession method for subsurface runoff was used to determine the subsurface runoff. The HEC-HMS model allows semi- and fully distributed approaches to schematisation of the watershed and rainfall situations. To determine the volume of runoff the method of SCS CN curves is used, which results depend on hydrological conditions of the soils. The rainfall-runoff model assuming selection of so-called methods of event of the SCS-CN type is used to determine the hydrograph and peak flow rate based on simulation of surface runoff in precipitation exceeding the infiltration capacity of the soil. The recession method is used to solve the baseflow (subsurface) runoff. The method is based on the separation of hydrograph to direct runoff and subsurface or baseflow runoff. The study area for the simulation of runoff using the method of SCS CN curves to determine the hydrological transformation is the Ploučnice basin. The Ploučnice is a hydrologically significant river in the northern part of the Czech Republic, it is a right tributary of the Elbe river with a total basin area of 1.194 km2. The average value of CN curves for the Ploučnice basin is 72. The geological structure of the Ploučnice basin is predominantly formed by Mesozoic sandstone. Despite significant initial loss of rainfall the basin response to the causal rainfall was demonstrated by a rapid rise of the surface runoff from the watershed and reached culmination flow. Basically, only surface runoff occures in the catchment during the initial phase of this extreme event. The increase of the baseflow runoff is slower and remains constant after reaching a certain level. The rise of the baseflow runoff is showed in a descending part of the hydrograph. The recession method in this case shows almost 20 hours delay. Results from the HEC-HMS prove availability of both methods for the runoff modeling in this type of catchment. When simulating extreme short-term rainfall-runoff episodes, the influence of geological subsurface is not significant, but it is manifested. Using more relevant rainfall events would bring more satisfactory results.

Quality of life after quitting smoking and initiating aerobic exercise.

PubMed

Bloom, Erika Litvin; Minami, Haruka; Brown, Richard A; Strong, David R; Riebe, Deborah; Abrantes, Ana M

2017-10-01

Quitting smoking and aerobic exercise each improve health. Although smokers may be concerned that quitting smoking will reduce their quality of life (QOL), recent research has shown that cessation is associated with QOL benefits. Elements of smoking cessation interventions, such as exercise, may contribute to changes in QOL. However, it is unknown whether initiating exercise in the context of smoking cessation is associated with greater or different effects on QOL than smoking cessation alone. The current study is a secondary analysis of data from a randomized trial (n = 61) of an exercise intervention for smoking cessation. We hypothesized that smoking abstinence and engagement in exercise would have positive, additive effects on QOL at end-of-treatment, 6- and, 12-month follow-ups. Sedentary adult smokers were randomized to the exercise intervention or a health education control (HEC) group. Additionally, all participants received smoking cessation counseling and nicotine patches. Data were analyzed using actual engagement in exercise, rather than group assignment as a proxy for exercise engagement, because some HEC participants also began exercising. Abstinence was positively associated with higher total and physical health QOL at follow-up. Exercise was not associated with total QOL and only marginally associated with physical health QOL, but was positively related to overall sense of well-being. Emphasizing that smoking cessation is associated with higher QOL may help motivate smokers to initiate quit attempts.

Numerical modeling of rapidly varying flows using HEC-RAS and WSPG models.

PubMed

Rao, Prasada; Hromadka, Theodore V

2016-01-01

The performance of two popular hydraulic models (HEC-RAS and WSPG) for modeling hydraulic jump in an open channel is investigated. The numerical solutions are compared with a new experimental data set obtained for varying channel bottom slopes and flow rates. Both the models satisfactorily predict the flow depths and location of the jump. The end results indicate that the numerical models output is sensitive to the value of chosen roughness coefficient. For this application, WSPG model is easier to implement with few input variables.

Inhibition of Expression of the S100A8 Gene Encoding the S100 Calcium-Binding Protein A8 Promotes Apoptosis by Suppressing the Phosphorylation of Protein Kinase B (Akt) in Endometrial Carcinoma and HEC-1A Cells.

PubMed

Liu, Chang; Xing, Guangyang; Wu, Cailiang; Zhu, Jun; Wei, Min; Liu, Dajiang; Ge, Yan; Chen, Yao; Lei, Ting; Yang, Yongxiu

2018-03-29

BACKGROUND The aim of this study was to investigate the expression and silencing of the S100A8 gene, which encodes the S100 calcium-binding protein A8 (S100A8), and apoptosis and phosphorylation of protein kinase B (Akt) in tissue samples of endometrial carcinoma and HEC-1A endometrial adenocarcinoma cells in vitro. MATERIAL AND METHODS Immunohistochemistry (IHC) was used to detect expression of the S100A8 protein in 74 tissue samples of endometrial cancer and 22 normal endometrial tissue samples. A stable S100A8 gene knockdown cell line was constructed using lentiviral packing short hairpin RNA (shRNA) transfected into HEC-1A cells. S100A8 mRNA and S100A8 protein levels were detected by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blotting. The effects of expression of the S100A8 gene by endometrial cancer cells was investigated by the MTT assay, cell cycle and apoptotic assays, qRT-PCR, and Western blotting. RESULTS IHC showed high levels of expression of S100A8 protein in endometrial carcinoma tissues, and HEC-1A adenocarcinoma cells (in G1 and G2). Increased expression of S100A8 protein was found endometrial cancer tissues compared with normal endometrial tissues (79.7% vs. 4.5%). S100A8 gene knockdown reduced cell proliferation in the HEC-1A cells compared with control cells, induced cell apoptosis, inhibited the phosphorylation of protein kinase B (Akt), and induced the expression of pro-apoptotic genes, including the cytochrome C gene, CYCS, BAD, BAX, FOXO1, FOXO3, CASP9, and CASP3. CONCLUSIONS In endometrial carcinoma cells, down-regulation of the S100A8 gene induced cell apoptosis via inhibition of the phosphorylated or active form of protein kinase B (Akt).

Inhibition of Expression of the S100A8 Gene Encoding the S100 Calcium-Binding Protein A8 Promotes Apoptosis by Suppressing the Phosphorylation of Protein Kinase B (Akt) in Endometrial Carcinoma and HEC-1A Cells

PubMed Central

Liu, Chang; Xing, Guangyang; Wu, Cailiang; Zhu, Jun; Wei, Min; Liu, Dajiang; Ge, Yan; Chen, Yao; Lei, Ting

2018-01-01

Background The aim of this study was to investigate the expression and silencing of the S100A8 gene, which encodes the S100 calcium-binding protein A8 (S100A8), and apoptosis and phosphorylation of protein kinase B (Akt) in tissue samples of endometrial carcinoma and HEC-1A endometrial adenocarcinoma cells in vitro. Material/Methods Immunohistochemistry (IHC) was used to detect expression of the S100A8 protein in 74 tissue samples of endometrial cancer and 22 normal endometrial tissue samples. A stable S100A8 gene knockdown cell line was constructed using lentiviral packing short hairpin RNA (shRNA) transfected into HEC-1A cells. S100A8 mRNA and S100A8 protein levels were detected by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blotting. The effects of expression of the S100A8 gene by endometrial cancer cells was investigated by the MTT assay, cell cycle and apoptotic assays, qRT-PCR, and Western blotting. Results IHC showed high levels of expression of S100A8 protein in endometrial carcinoma tissues, and HEC-1A adenocarcinoma cells (in G1 and G2). Increased expression of S100A8 protein was found endometrial cancer tissues compared with normal endometrial tissues (79.7% vs. 4.5%). S100A8 gene knockdown reduced cell proliferation in the HEC-1A cells compared with control cells, induced cell apoptosis, inhibited the phosphorylation of protein kinase B (Akt), and induced the expression of pro-apoptotic genes, including the cytochrome C gene, CYCS, BAD, BAX, FOXO1, FOXO3, CASP9, and CASP3. Conclusions In endometrial carcinoma cells, down-regulation of the S100A8 gene induced cell apoptosis via inhibition of the phosphorylated or active form of protein kinase B (Akt). PMID:29595187

Improving predictions of the effects of extreme events, land use, and climate change on the hydrology of watersheds in the Philippines

NASA Astrophysics Data System (ADS)

Benavidez, Rubianca; Jackson, Bethanna; Maxwell, Deborah; Paringit, Enrico

2016-05-01

Due to its location within the typhoon belt, the Philippines is vulnerable to tropical cyclones that can cause destructive floods. Climate change is likely to exacerbate these risks through increases in tropical cyclone frequency and intensity. To protect populations and infrastructure, disaster risk management in the Philippines focuses on real-time flood forecasting and structural measures such as dikes and retaining walls. Real-time flood forecasting in the Philippines mostly utilises two models from the Hydrologic Engineering Center (HEC): the Hydrologic Modeling System (HMS) for watershed modelling, and the River Analysis System (RAS) for inundation modelling. This research focuses on using non-structural measures for flood mitigation, such as changing land use management or watershed rehabilitation. This is being done by parameterising and applying the Land Utilisation and Capability Indicator (LUCI) model to the Cagayan de Oro watershed (1400 km2) in southern Philippines. The LUCI model is capable of identifying areas providing ecosystem services such as flood mitigation and agricultural productivity, and analysing trade-offs between services. It can also assess whether management interventions could enhance or degrade ecosystem services at fine spatial scales. The LUCI model was used to identify areas within the watershed that are providing flood mitigating services and areas that would benefit from management interventions. For the preliminary comparison, LUCI and HEC-HMS were run under the same scenario: baseline land use and the extreme rainfall event of Typhoon Bopha. The hydrographs from both models were then input to HEC-RAS to produce inundation maps. The novelty of this research is two-fold: (1) this type of ecosystem service modelling has not been carried out in the Cagayan de Oro watershed; and (2) this is the first application of the LUCI model in the Philippines. Since this research is still ongoing, the results presented in this paper are preliminary. As the land use and soil parameterisation for this watershed are refined and more scenarios are run through the model, more robust comparisons can be made between the hydrographs produced by LUCI and HEC-HMS and how those differences affect the inundation map produced by HEC-RAS.

Virulence genotypes and antimicrobial susceptibility patterns of Arcobacter butzleri isolated from seafood and its environment.

PubMed

Rathlavath, Srinu; Kohli, Vandita; Singh, Asem Sanjit; Lekshmi, Manjusha; Tripathi, Gayatri; Kumar, Sanath; Nayak, Binaya Bhusan

2017-12-18

Arcobacter butzleri is an emerging pathogen isolated from animals, food and the environment. In this study, 147 A. butzleri isolated from seafood and the coastal environment were tested for the presence of ten putative virulence genes (cadF, cj1349, ciaB, mviN, pldA, tlyA, hecA, hecB, irgA, iroE) and antimicrobial susceptibilities. Majority of the isolates harbored mviN (100%), cj1349 (97.2%), ciaB (95.9%), tlyA (91.8%), pldA (91.1%) and cadF (89.7%). Lower detection rates were observed for hecA (10.8%), hecB (19%), iroE (12.9%) and irgA (17.6%). Three A. butzleri isolates harbored all ten virulence genes. The occurrence of cj1349, ciaB, pldA, tlyA and hecA genes was significantly different (P≤0.05) among the isolates from different sources. All (100%) A. butzleri isolates were resistant to vancomycin, cephalothin, cefoxitin and sulphamethizole and susceptible to polymyxin-B, kanamycin, streptomycin, gentamicin, tetracycline and imipenem. Resistance to clinically important antibiotics such as cefotaxime (99.3%), ceftazidime (87.7%), nalidixic acid (70.7%), ampicillin (72.1%), ertapenem and amoxicillin-clavulanic acid (41.9%) was observed in A. butzleri from the environment. The isolates were highly susceptible to norfloxacin (97.9%) and colistin (97.2%), followed by ciprofloxacin (88.4%), meropenem (74.8%), chloramphenicol (72.7%) and erythromycin (69.3%). A. butzleri from different sources were not significantly different with respect to their antimicrobial susceptibility patterns. Multidrug resistance was observed in 66 (81.4%) isolates from fish, 29 (72.5%) isolates from shellfish and 17 (65.3%) isolates from coastal water. A. butzleri harboring virulence genes and resistance to multiple antibiotics found in seafood could be a potential health risk to seafood handlers and consumers. Continuous monitoring of seafood for potentially pathogenic A. butzleri is important to understand the evolution of antibiotic resistance in this emerging food pathogen and to determine the antimicrobial therapy regimen in the event of food-borne A. butzleri infections. Copyright © 2017 Elsevier B.V. All rights reserved.

Calibration of HEC-Ras hydrodynamic model using gauged discharge data and flood inundation maps

NASA Astrophysics Data System (ADS)

Tong, Rui; Komma, Jürgen

2017-04-01

The estimation of flood is essential for disaster alleviation. Hydrodynamic models are implemented to predict the occurrence and variance of flood in different scales. In practice, the calibration of hydrodynamic models aims to search the best possible parameters for the representation the natural flow resistance. Recent years have seen the calibration of hydrodynamic models being more actual and faster following the advance of earth observation products and computer based optimization techniques. In this study, the Hydrologic Engineering River Analysis System (HEC-Ras) model was set up with high-resolution digital elevation model from Laser scanner for the river Inn in Tyrol, Austria. 10 largest flood events from 19 hourly discharge gauges and flood inundation maps were selected to calibrate the HEC-Ras model. Manning roughness values and lateral inflow factors as parameters were automatically optimized with the Shuffled complex with Principal component analysis (SP-UCI) algorithm developed from the Shuffled Complex Evolution (SCE-UA). Different objective functions (Nash-Sutcliffe model efficiency coefficient, the timing of peak, peak value and Root-mean-square deviation) were used in single or multiple way. It was found that the lateral inflow factor was the most sensitive parameter. SP-UCI algorithm could avoid the local optimal and achieve efficient and effective parameters in the calibration of HEC-Ras model using flood extension images. As results showed, calibration by means of gauged discharge data and flood inundation maps, together with objective function of Nash-Sutcliffe model efficiency coefficient, was very robust to obtain more reliable flood simulation, and also to catch up with the peak value and the timing of peak.

Psychometric properties of the Persian version of the “Hospital Ethical Climate Survey”

PubMed Central

Khalesi, Nader; Arabloo, Jalal; Khosravizadeh, Omid; Taghizadeh, Sanaz; Heyrani, Ali; Ebrahimian, Abbasali

2014-01-01

In order to improve the ethical climate in health care organizations, it is important to apply a valid measure. This study aimed to investigate the psychometric properties of the Persian version of the Hospital Ethical Climate Survey (HECS) and to assess nurses’ perceptions of the ethical climate in teaching hospitals of Iran. A cross-sectional study of randomly selected nurses (n = 187) was conducted in three teaching general hospitals of Tehran, capital of Iran. Olson’s Hospital Ethical Climate Survey (HECS), a self-administered questionnaire, was used to assess the nurses’ perceptions of the hospital ethical climate. Descriptive statistics, confirmatory factor analysis (CFA), internal consistency, and correlation were used to analyze the data. CFA showed acceptable model fit: an standardized root mean square residual (SRMR) of 0.064, an non-normalized fit index (NNFI) of 0.96, a comparative fit index (CFI) of 0.96, and an root mean square error of approximation (RMSEA) of 0.075. The Cronbach’s alpha values were acceptable and ranging from 0.69 to 0.85. The overall Cronbach’s alpha coefficient was 0.94. The factor loadings for all ethical climate items were between 0.50 and 0.80, which revealed good structure of the Persian version of the HECS. Survey findings showed that the “managers” subscale had the highest score and the subscale of “doctors” had the lowest score. This study shows that the Persian version of the HECS is a valid and reliable instrument for measuring nurses’ perceptions of the ethical climate in hospitals of Iran PMID:25512834

New configuration for efficient and durable copper coating on the outer surface of a tube

DOE PAGES

Ahmad, Irfan; Chapman, Steven F.; Velas, Katherine M.; ...

2017-03-27

A well-adhered copper coating on stainless steel power coupler parts is required in superconducting radio frequency (SRF) accelerators. Radio frequency power coupler parts are complex, tubelike stainless steel structures, which require copper coating on their outer and inner surfaces. Conventional copper electroplating sometimes produces films with inadequate adhesion strength for SRF applications. Electroplating also requires a thin nickel strike layer under the copper coating, whose magnetic properties can be detrimental to SRF applications. Coaxial energetic deposition (CED) and sputtering methods have demonstrated efficient conformal coating on the inner surfaces of tubes but coating the outer surface of a tube ismore » challenging because these coating methods are line of sight. When the substrate is off axis and the plasma source is on axis, only a small section of the substrate’s outer surface is exposed to the source cathode. The conventional approach is to rotate the tube to achieve uniformity across the outer surface. This method results in poor film thickness uniformity and wastes most of the source plasma. Alameda Applied Sciences Corporation (AASC) has developed a novel configuration called hollow external cathode CED (HEC-CED) to overcome these issues. HEC-CED produces a film with uniform thickness and efficiently uses all eroded source material. Furthermore, the Cu film deposited on the outside of a stainless steel tube using the new HEC-CED configuration survived a high pressure water rinse adhesion test. HEC-CED can be used to coat the outside of any cylindrical structure.« less

Breathing awareness meditation and LifeSkills Training programs influence upon ambulatory blood pressure and sodium excretion among African American adolescents.

PubMed

Gregoski, Mathew J; Barnes, Vernon A; Tingen, Martha S; Harshfield, Gregory A; Treiber, Frank A

2011-01-01

To evaluate the effect of breathing awareness meditation (BAM), Botvin LifeSkills Training (LST), and health education control (HEC) on ambulatory blood pressure and sodium excretion in African American adolescents. Following 3 consecutive days of systolic blood pressure (SBP) screenings, 166 eligible participants (i.e., SBP >50th-95th percentile) were randomized by school to either BAM (n = 53), LST (n = 69), or HEC (n = 44). In-school intervention sessions were administered for 3 months by health education teachers. Before and after the intervention, overnight urine samples and 24-hour ambulatory SBP, diastolic blood pressure, and heart rate were obtained. Significant group differences were found for changes in overnight SBP and SBP, diastolic blood pressure, and heart rate over the 24-hour period and during school hours. The BAM treatment exhibited the greatest overall decreases on these measures (Bonferroni adjusted, ps < .05). For example, for school-time SBP, BAM showed a change of -3.7 mmHg compared with no change for LST and a change of -.1 mmHg for HEC. There was a nonsignificant trend for overnight urinary sodium excretion (p = .07), with the BAM group displaying a reduction of -.92 ± 1.1 mEq/hr compared with increases of .89 ± 1.2 mEq/hr for LST and .58 ± .9 mEq/hr for HEC group. BAM appears to improve hemodynamic function and may affect sodium handling among African American adolescents who are at increased risk for development of cardiovascular disease. Published by Elsevier Inc.

Influences of neutralization of superabsorbent hydrogel from hydroxyethyl cellulose on water swelling capacities

NASA Astrophysics Data System (ADS)

Adair, Ajaman; Klinpituksa, Pairote; Kaesaman, Azizon

2017-08-01

In this research, superabsorbent hydrogels were synthesized by graft copolymerization of hydroxyethyl cellulose (HEC) and polyacrylamide (PAM) under the initiation of potassium persulfate (KPS). The polymer networks were constructed using N,N'-methylenebisacrylamide (MBA), and the reaction was performed in an aqueous solution. The extent of grafting products was evaluated form grafting efficiency (%GE) and percentage of add-ons at HEC/AM ratios of 1: 10. The water swelling capacities, in terms of swelling capacity and weight loss, of resultant superabsorbent polymers (SAPs) after solvent extraction were determined for swelling behaviors. The result showed that the SAP had poor water absorption of approximately up to 23 g/g. To enhance swelling capacity of SAPs, an alkaline hydrolysis was done by using two types of alkaline bases, i.e., 2 M NaOH and 2 M KOH solution. The obtained treatment SAPs were neutralized by washing with distilled water and 0.5 M HCl until the liquors pH was nearly 7. They were found that the treatment SAPs showed the highest water absorption up to 317 g/g. Influences of various fluids pH values ranging between 4 and 10, on water swelling capacities of SAPs were also investigated. Under optimal pH value, the highest water absorptions of SAP was 382 g/g. To confirm the grafting reaction of PAM onto HEC backbone, FT-IR analysis was used. The results revealed absorption bands of the HEC backbone and new absorption bands from the grafted copolymer. Furthermore, the FT-IR spectrum was proved that washing with distilled water can alter the chemical functional group of SAPs.

New configuration for efficient and durable copper coating on the outer surface of a tube

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ahmad, Irfan; Chapman, Steven F.; Velas, Katherine M.

A well-adhered copper coating on stainless steel power coupler parts is required in superconducting radio frequency (SRF) accelerators. Radio frequency power coupler parts are complex, tubelike stainless steel structures, which require copper coating on their outer and inner surfaces. Conventional copper electroplating sometimes produces films with inadequate adhesion strength for SRF applications. Electroplating also requires a thin nickel strike layer under the copper coating, whose magnetic properties can be detrimental to SRF applications. Coaxial energetic deposition (CED) and sputtering methods have demonstrated efficient conformal coating on the inner surfaces of tubes but coating the outer surface of a tube ismore » challenging because these coating methods are line of sight. When the substrate is off axis and the plasma source is on axis, only a small section of the substrate’s outer surface is exposed to the source cathode. The conventional approach is to rotate the tube to achieve uniformity across the outer surface. This method results in poor film thickness uniformity and wastes most of the source plasma. Alameda Applied Sciences Corporation (AASC) has developed a novel configuration called hollow external cathode CED (HEC-CED) to overcome these issues. HEC-CED produces a film with uniform thickness and efficiently uses all eroded source material. Furthermore, the Cu film deposited on the outside of a stainless steel tube using the new HEC-CED configuration survived a high pressure water rinse adhesion test. HEC-CED can be used to coat the outside of any cylindrical structure.« less

EphA2 Targeted Chemotherapy Using an Antibody Drug Conjugate in Endometrial Carcinoma

PubMed Central

Lee, Jeong-Won; Stone, Rebecca L.; Lee, Sun Joo; Nam, Eun Ji; Roh, Ju-Won; Nick, Alpa M.; Han, Hee-Dong; Shahzad, Mian M.K.; Kim, Hye-Sun; Mangala, Lingegowda S.; Jennings, Nicholas B.; Mao, Shenlan; Gooya, John; Jackson, Dowdy; Coleman, Robert L.; Sood, Anil K.

2013-01-01

Purpose EphA2 overexpression is frequently observed in endometrial cancers, and is predictive of poor clinical outcome. Here, we utilize an antibody drug conjugate (MEDI-547) composed of a fully human monoclonal antibody against both human and murine EphA2 (1C1) and the tubulin polymerization inhibitor, monomethylauristatin F (MMAF). Experimental design EphA2 expression was examined in endometrial cancer cell lines by Western Blot. Specificity of MEDI-547 was examined by antibody degradation and internalization assays. Viability and apoptosis were investigated in endometrial cancer cell lines and orthotopic tumor models. Results EphA2 was expressed in the Hec-1A and Ishikawa cells, but was absent in the SPEC-2 cells. Antibody degradation and internalization assays showed that the antibody drug conjugate decreased EphA2 protein levels and was internalized in EphA2 positive cells (Hec-1A and Ishikawa). Moreover, in vitro cytotoxicity and apoptosis assays demonstrated that the antibody drug conjugate decreased viability and increased apoptosis of Hec-1A and Ishikawa cells. In vivo therapy experiments in mouse orthotopic models with this antibody drug conjugate resulted in 86 to 88% growth inhibition (P < 0.001) in the orthotopic Hec-1A and Ishikawa models compared to controls. Moreover, the mice treated with this antibody drug conjugate had a lower incidence of distant metastasis compared with controls. The anti-tumor effects of the therapy were related to decreased proliferation and increased apoptosis of tumor and associated endothelial cells. Conclusions The preclinical data for endometrial cancer treatment using MEDI-547 demonstrate substantial anti-tumor activity. PMID:20388851

Hydrogel-based electrochemical sensor for non-invasive and continuous glucose monitoring

NASA Astrophysics Data System (ADS)

Park, Habeen; Lee, Ji-Young; Kim, Dong-Chul; Koh, Younggook; Cha, Junhoe

2017-07-01

Monitoring blood glucose level of diabetic patients is crucial in diabetes care from life threating complications. Selfmonitoring blood glucose (SMBG) that involves finger prick to draw blood samples into the measurement system is a widely-used method of routine measurement of blood glucose levels to date. SMBG includes, however, unavoidable pain problems resulting from the repetitive measurements. We hereby present a hydrogel-based electrochemical (H-EC) sensor to monitor the glucose level, non-invasively. Glucose oxidase (GOx) was immobilized in the disc-type hydroxyethyl methacrylate (HEMA) based hydrogel and kept intact in the hydrogel. Fast electron transfer mediated by Prussian blue (PB, hexacyanoferrate) generated efficient signal amplifications to facilitate the detection of the extracted glucose from the interstitial fluid. The linear response and the selectivity against glucose of the H-EC sensor were validated by chronoamperometry. For the practical use, the outcomes from the correlation of the extracted glucose concentration and the blood glucose value by on-body extraction, as well as the validation of the hydrogel-based electrochemical (H-EC) device, were applied to the on-body glucose monitoring.

On the Role of Specific Interactions in the Diffusion of Nanoparticles in Aqueous Polymer Solutions

PubMed Central

2013-01-01

Understanding nanoparticle diffusion within non-Newtonian biological and synthetic fluids is essential in designing novel formulations (e.g., nanomedicines for drug delivery, shampoos, lotions, coatings, paints, etc.), but is presently poorly defined. This study reports the diffusion of thiolated and PEGylated silica nanoparticles, characterized by small-angle neutron scattering, in solutions of various water-soluble polymers such as poly(acrylic acid) (PAA), poly(N-vinylpyrrolidone) (PVP), poly(ethylene oxide) (PEO), and hydroxyethylcellulose (HEC) probed using NanoSight nanoparticle tracking analysis. Results show that the diffusivity of nanoparticles is affected by their dimensions, medium viscosity, and, in particular, the specific interactions between nanoparticles and the macromolecules in solution; strong attractive interactions such as hydrogen bonding hamper diffusion. The water-soluble polymers retarded the diffusion of thiolated particles in the order PEO > PVP > PAA > HEC whereas for PEGylated silica particles retardation followed the order PAA > PVP = HEC > PEO. In the absence of specific interactions with the medium, PEGylated nanoparticles exhibit enhanced mobility compared to their thiolated counterparts despite some increase in their dimensions. PMID:24354390

On the role of specific interactions in the diffusion of nanoparticles in aqueous polymer solutions.

PubMed

Mun, Ellina A; Hannell, Claire; Rogers, Sarah E; Hole, Patrick; Williams, Adrian C; Khutoryanskiy, Vitaliy V

2014-01-14

Understanding nanoparticle diffusion within non-Newtonian biological and synthetic fluids is essential in designing novel formulations (e.g., nanomedicines for drug delivery, shampoos, lotions, coatings, paints, etc.), but is presently poorly defined. This study reports the diffusion of thiolated and PEGylated silica nanoparticles, characterized by small-angle neutron scattering, in solutions of various water-soluble polymers such as poly(acrylic acid) (PAA), poly(N-vinylpyrrolidone) (PVP), poly(ethylene oxide) (PEO), and hydroxyethylcellulose (HEC) probed using NanoSight nanoparticle tracking analysis. Results show that the diffusivity of nanoparticles is affected by their dimensions, medium viscosity, and, in particular, the specific interactions between nanoparticles and the macromolecules in solution; strong attractive interactions such as hydrogen bonding hamper diffusion. The water-soluble polymers retarded the diffusion of thiolated particles in the order PEO > PVP > PAA > HEC whereas for PEGylated silica particles retardation followed the order PAA > PVP = HEC > PEO. In the absence of specific interactions with the medium, PEGylated nanoparticles exhibit enhanced mobility compared to their thiolated counterparts despite some increase in their dimensions.

Estimating design flood and HEC-RAS modelling approach for flood analysis in Bojonegoro city

NASA Astrophysics Data System (ADS)

Prastica, R. M. S.; Maitri, C.; Hermawan, A.; Nugroho, P. C.; Sutjiningsih, D.; Anggraheni, E.

2018-03-01

Bojonegoro faces flood every year with less advanced prevention development. Bojonegoro city development could not peak because the flood results material losses. It affects every sectors in Bojonegoro: education, politics, economy, social, and infrastructure development. This research aims to analyse and to ensure that river capacity has high probability to be the main factor of flood in Bojonegoro. Flood discharge analysis uses Nakayasu synthetic unit hydrograph for period of 5 years, 10 years, 25 years, 50 years, and 100 years. They would be compared to the water maximum capacity that could be loaded by downstream part of Bengawan Solo River in Bojonegoro. According to analysis result, Bengawan Solo River in Bojonegoro could not able to load flood discharges. Another method used is HEC-RAS analysis. The conclusion that shown by HEC-RAS analysis has the same view. It could be observed that flood water loading is more than full bank capacity elevation in the river. To conclude, the main factor that should be noticed by government to solve flood problem is river capacity.

A TPR domain–containing N-terminal module of MPS1 is required for its kinetochore localization by Aurora B

PubMed Central

Nijenhuis, Wilco; von Castelmur, Eleonore; Littler, Dene; De Marco, Valeria; Tromer, Eelco; Vleugel, Mathijs; van Osch, Maria H.J.; Snel, Berend

2013-01-01

The mitotic checkpoint ensures correct chromosome segregation by delaying cell cycle progression until all kinetochores have attached to the mitotic spindle. In this paper, we show that the mitotic checkpoint kinase MPS1 contains an N-terminal localization module, organized in an N-terminal extension (NTE) and a tetratricopeptide repeat (TPR) domain, for which we have determined the crystal structure. Although the module was necessary for kinetochore localization of MPS1 and essential for the mitotic checkpoint, the predominant kinetochore binding activity resided within the NTE. MPS1 localization further required HEC1 and Aurora B activity. We show that MPS1 localization to kinetochores depended on the calponin homology domain of HEC1 but not on Aurora B–dependent phosphorylation of the HEC1 tail. Rather, the TPR domain was the critical mediator of Aurora B control over MPS1 localization, as its deletion rendered MPS1 localization insensitive to Aurora B inhibition. These data are consistent with a model in which Aurora B activity relieves a TPR-dependent inhibitory constraint on MPS1 localization. PMID:23569217

A TPR domain-containing N-terminal module of MPS1 is required for its kinetochore localization by Aurora B.

PubMed

Nijenhuis, Wilco; von Castelmur, Eleonore; Littler, Dene; De Marco, Valeria; Tromer, Eelco; Vleugel, Mathijs; van Osch, Maria H J; Snel, Berend; Perrakis, Anastassis; Kops, Geert J P L

2013-04-15

The mitotic checkpoint ensures correct chromosome segregation by delaying cell cycle progression until all kinetochores have attached to the mitotic spindle. In this paper, we show that the mitotic checkpoint kinase MPS1 contains an N-terminal localization module, organized in an N-terminal extension (NTE) and a tetratricopeptide repeat (TPR) domain, for which we have determined the crystal structure. Although the module was necessary for kinetochore localization of MPS1 and essential for the mitotic checkpoint, the predominant kinetochore binding activity resided within the NTE. MPS1 localization further required HEC1 and Aurora B activity. We show that MPS1 localization to kinetochores depended on the calponin homology domain of HEC1 but not on Aurora B-dependent phosphorylation of the HEC1 tail. Rather, the TPR domain was the critical mediator of Aurora B control over MPS1 localization, as its deletion rendered MPS1 localization insensitive to Aurora B inhibition. These data are consistent with a model in which Aurora B activity relieves a TPR-dependent inhibitory constraint on MPS1 localization.

Mucosal integrity and inflammatory markers in the female lower genital tract as potential screening tools for vaginal microbicides.

PubMed

Su, H Irene; Schreiber, Courtney A; Fay, Courtney; Parry, Sam; Elovitz, Michal A; Zhang, Jian; Shaunik, Alka; Barnhart, Kurt

2011-11-01

In the female genital tract, vaginal colposcopy, endometrial mucosal integrity and inflammatory mediators are potential in vivo biomarkers of microbicide and contraceptive safety. A randomized, blinded crossover trial of 18 subjects comparing effects of nonoxynol-9 vaginal gel (Gynol II; putative inflammatory gel), hydroxyethyl cellulose gel (HEC; putative inert gel) and no gel exposure on endometrial and vaginal epithelial integrity and endometrial and vaginal inflammatory markers [interleukin (IL) 1β, IL-6, IL-8, MCP-1, MIP-1α, MIP-1β, RANTES, tumor necrosis factor α, IL-1RA, IL-10, SLPI). Gynol II was associated with more vaginal lesions. No endometrial disruptions were observed across conditions. In the vagina, RANTES (p=.055) and IL-6 (p=.04) were higher after HEC exposure than at baseline. In the endometrium, IL-1β (p=.003) and IL-8 (p=.025) were lower after Gynol II cycles than after no gel. Gynol II and HEC may modulate inflammatory markers in the vagina and endometrium. How these changes relate to infection susceptibility warrants further study. Copyright © 2011 Elsevier Inc. All rights reserved.

Molecular weight distribution characterization of hydrophobe-modified hydroxyethyl cellulose by size-exclusion chromatography.

PubMed

Li, Yongfu; Meunier, David M; Partain, Emmett M

2014-09-12

Size-exclusion chromatography (SEC) of hydrophobe-modified hydroxyethyl cellulose (HmHEC) is challenging because polymer chains are not isolated in solution due to association of hydrophobic groups and hydrophobic interaction with column packing materials. An approach to neutralize these hydrophobic interactions was developed by adding β-cyclodextrin (β-CD) to the aqueous eluent. SEC mass recovery, especially for the higher molecular weight chains, increased with increasing concentration of β-CD in the eluent. A β-CD concentration of 0.75wt% in the eluent was determined to be optimal for the HmHEC polymers studied. These conditions enabled precise determinations of apparent molecular weight distributions exhibiting less than 2% relative standard deviation in the measured weight-average molecular weight (MW) for five injections on three studied samples and showed no significant differences in MW determined on two different days. The developed technology was shown to be very robust for characterizing HmHEC having MW from 500kg/mol to 2000kg/mol, and it can be potentially applied to other hydrophobe-modified polymers. Copyright © 2014 Elsevier B.V. All rights reserved.

Sleep patterns during rearing under different environmental conditions in juvenile rats.

PubMed

Mirmiran, M; van den Dungen, H; Uylings, H B

1982-02-11

Male Wistar rats were chronically implanted at weaning with electrodes for recording EEG amd EMG. From one month of age, they were randomly assigned to either 24 h or 2 h daily 'enriched' (EC), 'standard' (SC) or 'isolated' (IC) rearing conditions. The 24 h-EC group showed the following changes with respect to both the SC and the IC groups: (a) more quiet sleep time; (b) more active sleep time, and (c) shorter active sleep latency. These differences were evident by the third week of environmental conditioning, became statistically significant by 4 weeks, and continued to increase throughout the rest of the enrichment period. None of the sleep parameters showed any significant differences between the SC and the IC groups. The 2 h-EC rats showed an increase in both quiet sleep and active sleep time during the experimental period, whereas the controls showed the expected decline. Morphological investigations at 3 months of age revealed a significant weight increase in the cerebral cortex, and even more so in the hypothalamus as compared with the control group in the 2 h-EC rats.

Design, characterization and evaluation of hydroxyethylcellulose based novel regenerable supersorbent for heavy metal ions uptake and competitive adsorption.

PubMed

Abbas, Azhar; Hussain, Muhammad Ajaz; Sher, Muhammad; Irfan, Muhammad Imran; Tahir, Muhammad Nawaz; Tremel, Wolfgang; Hussain, Syed Zajif; Hussain, Irshad

2017-09-01

Hydroxyethylcellulose succinate-Na (HEC-Suc-Na) was designed and evaluated for removal of some heavy metal ions from aqueous solution. Pristine sorbent HEC-Suc-Na was thoroughly characterized by FTIR and solid-state CP/MAS 13 C NMR spectroscopy, SEM-EDS and zero point charge analyses. Langmuir isotherm, pseudo second order kinetic and ion exchange models provided best fit to the experimental data of sorption of metal ions. Maximum sorption capacities of supersorbent HEC-Suc-Na for sorption of heavy metal ions from aqueous solution as calculated by Langmuir isotherm model were found to be 1000, 909.09, 666.6, 588 and 500mgg -1 for Pb(II), Cr(VI), Co(II), Cu(II) and Ni(II), respectively. Competitive sorption of these heavy metal ions was carried out from galvanic and nuclear waste water simulated environment. The negative values of ΔG° and ΔH° indicated spontaneity and exothermic nature of sorption. The sorbent was efficiently regenerated with no significant decrease in sorption capacity after five cycles. Copyright © 2017 Elsevier B.V. All rights reserved.

1D and 2D urban dam-break flood modelling in Istanbul, Turkey

NASA Astrophysics Data System (ADS)

Ozdemir, Hasan; Neal, Jeffrey; Bates, Paul; Döker, Fatih

2014-05-01

Urban flood events are increasing in frequency and severity as a consequence of several factors such as reduced infiltration capacities due to continued watershed development, increased construction in flood prone areas due to population growth, the possible amplification of rainfall intensity due to climate change, sea level rise which threatens coastal development, and poorly engineered flood control infrastructure (Gallegos et al., 2009). These factors will contribute to increased urban flood risk in the future, and as a result improved modelling of urban flooding according to different causative factor has been identified as a research priority (Gallegos et al., 2009; Ozdemir et al. 2013). The flooding disaster caused by dam failures is always a threat against lives and properties especially in urban environments. Therefore, the prediction of dynamics of dam-break flows plays a vital role in the forecast and evaluation of flooding disasters, and is of long-standing interest for researchers. Flooding occurred on the Ayamama River (Istanbul-Turkey) due to high intensity rainfall and dam-breaching of Ata Pond in 9th September 2009. The settlements, industrial areas and transportation system on the floodplain of the Ayamama River were inundated. Therefore, 32 people were dead and millions of Euros economic loses were occurred. The aim of this study is 1 and 2-Dimensional flood modelling of the Ata Pond breaching using HEC-RAS and LISFLOOD-Roe models and comparison of the model results using the real flood extent. The HEC-RAS model solves the full 1-D Saint Venant equations for unsteady open channel flow whereas LISFLOOD-Roe is the 2-D shallow water model which calculates the flow according to the complete Saint Venant formulation (Villanueva and Wright, 2006; Neal et al., 2011). The model consists a shock capturing Godunov-type scheme based on the Roe Riemann solver (Roe, 1981). 3 m high resolution Digital Surface Model (DSM), natural characteristics of the pond and its breaching such as depth, wide, length, volume and breaching shape and daily total rainfall data were used in the models. The simulated flooding in the both models were compared with the real flood extent which gathered from photos taken after the flood event, high satellite images acquired after 20 days from the flood event, and field works. The results show that LISFLOOD-Roe hydraulic model gives more than 80% fit to the extent of real flood event. Also both modelling results show that the embankment breaching of the Ata Pond directly affected the flood magnitude and intensity on the area. This study reveals that modelling of the probable flooding in urban areas is necessary and very important in urban planning. References Gallegos, H. A., Schubert, J. E., and Sanders, B. F.: Two dimensional, high-resolution modeling of urban dam-break flooding: A case study of Baldwin Hills California, Adv. Water Resour., 32, 1323-1335, 2009. Neal, J., Villanueva, I., Wright, N., Willis, T., Fewtrell, T. and Bates, P.: How mush physical complexity is needed to model flood inundation? Hydrological Processes, DOI: 10.1002/hyp.8339. Ozdemir H., Sampson C., De Almeida G., Bates P.D.: Evaluating scale and roughness effects in urban flood modelling using terrestrial LiDAR data, Hydrology and Earth System Sciences, vol.17, pp.4015-4030, 2013. Roe P.: Approximate Riemann solvers, parameter vectors, and difference-schemes. Journal of Computational Physics 43(2): 357-372, 1981. Villanueva I, Wright NG.: Linking Riemann and storage cell models for flood prediction. Proceedings of the Institution of Civil Engineers, Journal of Water Management 159: 27-33, 2006.

Mechanistic evaluation of alginate-HEC gelisphere compacts for controlled intrastriatal nicotine release in Parkinson's disease.

PubMed

Choonara, Yahya E; Pillay, Viness; Khan, Riaz A; Singh, Neha; du Toit, Lisa C

2009-06-01

This study focused on elucidating a mechanistic understanding in support of the multiple mechanisms which govern the formation of crosslinked alginate-hydroxyethylcellulose (Alg-HEC) gelispheres intended for the controlled intrastriatal release of nicotine as a neuroprotectant in Parkinson's Disease. HEC was incorporated as a reinforcing "protective" colloidal polymer to induce interactions between the free carboxyl groups of alginate with hydroxylated HEC monomers. Gelispheres were compressed within an external poly(lactic-co-glycolic acid) (PLGA) matrix to further prolong the release of nicotine. Sol-gel interconversion mechanisms, matrix deformability moduli, matrix fracture energies and chemometric models of the associated energy paradigms were analyzed for their influence on the mechanism and extent of nicotine release. Textural profiling demonstrated higher fracture energies (7.94-26.69 x 10(-4) J) and lower deformability moduli (12.24-58.36 N/mm) when gelispheres were cured in 2 M HCl as a postcuring step. Ba(2+) crosslinked gelispheres resulted in superiorly compact matrices with an increase in volume of 201-329% as compared to the Ca(2+) and Zn(2+) crosslinked matrices. The order of matrix compactness was as follows: Zn(2+) < Ca(2+) < Ba(2+). Molecular mechanisms of formation, interaction, conversion, and stability of sol-gel transitions depended on the type of crosslinker, crosslinking time, energy transactions, and interactions with molecules of the hydration medium. Ba(2+) crosslinked gelispheres released nicotine slower than Ca(2+) and Zn(2+) crosslinked gelispheres due to the higher energy requirement for interconversion to sol while the energy requirements for Ca(2+) and Zn(2+) was at a lower demand. Ba(2+) crosslinked gelispheres within PLGA matrices therefore retarded nicotine release in a pseudo-zero-order manner over 21 days. (c) 2008 Wiley-Liss, Inc.

Properties of thin film radiation detectors and their application to dosimetry and quality assurance in x-ray imaging

NASA Astrophysics Data System (ADS)

Elshahat, Bassem

The characteristics of two different types of thin-film radiation detectors are experimentally investigated: organic photovoltaic cells (OPV) and a new self-powered detector that operates based on high-energy secondary electrons (HEC). Although their working principles are substantially different, they both can be used for radiation detection and image formation in medical applications. OPVs with different active layer material thicknesses and aluminum electrode areas were fabricated. The OPV cell consisted of P3HT: PCBM photoactive materials, composed of donor and acceptor semiconducting organic materials, sandwiched between an aluminum electrode as anode and an indium tin oxide (ITO) electrode as a cathode. The detectors were exposed to 60150 kVp x rays, which generated photocurrent in the active layer. The electric charge production in the OPV cells was measured. The net current as function of beam energy (kVp) was proportional to ~1/kVp0.45 when adjusted for x-ray beam output. The best combination of parameters for these cells was 270-nm active layer thicknesses for 0.7cm-2 electrode area. The measured current ranged from about 0.7 to 2.4 nA/cm2 for 60-150 kVp, corresponding to about 0.09 -- 0.06 nA/cm2/mGy, respectively, when adjusted for the output x-ray source flux. The HEC detection concept was recently proposed and experimentally demonstrated by a UML/HMS research group. HEC detection employs direct conversion of high-energy electron current to detector signal without external power and amplification. The potential of using HEC detectors for diagnostic imaging application was investigated by using a heterogeneous phantom consisting of a water cylinder with Al and wax rod inserts.

Estimation of Channel-Forming Discharge and Large-Event Geomorphic Response Using HEC-RAS

NASA Astrophysics Data System (ADS)

Hamilton, P.; Strom, K.; Hosseiny, S. M. H.

2015-12-01

The goal of the present work was to consider the functionality and applicability of HEC-RAS sediment transport simulations in two situations. The first was as a mode for obtaining quick estimates of the effective discharge, one measure of channel-forming discharge, and the second was as a mode to quickly estimate sediment transport and the commensurate potential erosion and deposition during large flood events. Though there are many other sediment transport and morphodynamic models available, e.g., CCHE1D, Nays2DH, we were interested in using HEC-RAS since this is the model of choice for many regulatory bodies, e.g., FEMA, cities, and counties. This makes using the sediment transport capability of HEC-RAS a natural extension of models that already otherwise exist and are well calibrated. In first looking at the utility of these models, we wanted to estimate the effective discharge of streams. Effective discharge is one way of defining the channel-forming discharge for a stream and is therefore an important parameter in natural channel design and restoration efforts. By running this range of floods, one can easily obtain an estimate for recurrence interval most responsible for moving the majority of sediment over a long time period. Results were compared to data collected within our research group on the Brazos River (TX). Effective discharge is an important estimate, particularly in understanding the equilibrium channel condition. Nevertheless, large floods are contemporaneously catastrophic and understanding their potential effects is desirable. Finally, we performed some sensitivity analysis to better understand the underlying assumptions of the various sediment transport model options and how they might affect the outcome of the aforementioned computations.

The Photocatalytic Application of Semiconductor Stibnite Nanostructure Synthesized via a Simple Microwave-Assisted Approach in Propylene Glycol for Degradation of Dye Pollutants and its Optical Property

NASA Astrophysics Data System (ADS)

Saksornchai, Eksuree; Kavinchan, Jutarat; Thongtem, Somchai; Thongtem, Titipun

2017-11-01

Stibnite (Sb2S3) semiconducting material was successfully synthesized by a rapid and facile microwave route using antimony chloride (SbCl3) and sodiumthiosulfate (Na2S2O3) dissolved in propylene glycol (PG) containing different hydroxyethyl cellulose (HEC) masses. The phase identification, morphology, and elemental composition of products were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), field- emission scanning electron microscopy (FE-SEM), energy dispersive X-ray (EDX) spectroscopy, and Fourier transform infrared spectroscopy (FTIR). The results revealed the orthorhombic phase of Sb2S3 single crystal-forming sheaf-like nanostructure, and a possible formation mechanism was proposed and discussed. Its direct band gap calculated from UV-visible absorption is 1.60 eV. In this research, the photocatalytic activities of Sb2S3 nanostructure were investigated through the degradation of methyl orange (MO) and methylene blue (MB) under visible light irradiation. The as-obtained 0.30 g HEC-added solution (0.3 HEC-Sb2S3) photocatalyst exhibited better photocatalytic activity than the other products, which degraded 91% of MO within 300 min and 90% of MB within 240 min under the Xe-lamp irradiation. The first-order plot was fitted with this experiment which the rate constant ( k) of 0.3 HEC-Sb2S3 for MO and MB degradation are 0.0085 and 0.0098 min-1, respectively. Therefore, the new experience with a novel and simple synthetic procedure of Sb2S3 photocatalyst that exhibits the characteristics of a highly effective photocatalyst under visible light irradiation was discovered.

Changing stakeholder relationships in nature reserve management: a case study on Snake Island-Laotie Mountain National Nature Reserve, Liaoning, China.

PubMed

Zhou, Dan; Wang, Ziyan; Lassoie, James; Wang, Xiaoping; Sun, Lixin

2014-12-15

The number and total area of nature reserves in China has increased rapidly over the past couple of decades; however, the ability to effectively manage these reserves has not kept pace and conflicts between conservation efforts and economic development have emerged. The Snake Island-Laotie Mountain National Nature Reserve (SILMNNR) currently is experiencing the challenges of balancing conservation with local community development. This paper uses components analysis of human ecosystems (HEC) to examine conflicts arising from the management of the nature reserve and uses a stakeholder analysis to identify and better understand stakeholder inter-relationships in the SILMNNR-HEC. The goal of this study is to identify critical factors influencing stakeholder relationships in order to find ways of relieving conflicts between the reserve management and development. The stakeholder analysis revealed that the key stakeholders in the SILMNNR-HEC are natural resources, the Liaoning SILMNNR Authority, local residents, and enterprise developers; however, there was unequal power among stakeholders in the decision making process affecting the nature reserve. The paper evaluated the conditions and processes of SILMNNR-HEC through a framework of stakeholder relationships where critical factors, such as policy, finance, technology, and labor, and their respective strengths and feedbacks among stakeholders, were assessed and showed unequal flows of power among stakeholders. Two approaches are provided for transforming the unbalanced relationships into a stable and sustainable framework to sustainably manage the nature reserve: the first is by changing stakeholder relationships from opposition to cooperation; and the second by enhancing feedbacks and dynamics among stakeholders. The analysis used in this paper can be used as a model to assess conflicts around other protected areas in China and elsewhere. Copyright © 2014 Elsevier Ltd. All rights reserved.

Prevalence of Putative Virulence Genes in Campylobacter and Arcobacter Species Isolated from Poultry and Poultry By-Products in Tunisia.

PubMed

Jribi, Hela; Sellami, Hanen; Hassena, Amal Ben; Gdoura, Radhouane

2017-10-01

Campylobacter and Arcobacter spp. are common causes of gastroenteritis in humans; these infections are commonly due to undercooked poultry. However, their virulence mechanism is still poorly understood. The aim of this study was to evaluate the presence of genotypic virulence markers in Campylobacter and Arcobacter species using PCR. The prevalence of virulence and cytolethal distending toxin (CDT) genes was estimated in 71 Campylobacteraceae isolates. PCR was used to detect the presence of virulence genes (iam, cadF, virB1, flaA, cdtA, cdtB, and cdtC) using specific primers for a total of 45 Campylobacter isolates, including 37 C. jejuni and 8 C. coli. All the Campylobacter isolates were positive for the cadF gene. The plasmid gene virB11 was not detected in any strain. The invasion associated marker was not detected in C. jejuni. Lower detection rates were observed for flaA, cdtA, cdtB, and cdtC. The presence of nine putative Arcobacter virulence genes (cadF, ciaB, cj1349, mviN, pldA, tlyA, irgA, hecA, and hecB) was checked in a set of 22 Arcobacter butzleri and 4 Arcobacter cryaerophilus isolates. The pldA and mviN genes were predominant (88.64%). Lower detection rates were observed for tlyA (84.76%), ciaB (84.61%), cadF and cj1349 (76.92%), IrgA and hecA (61.53%), and hecB (57.69%). The findings revealed that a majority of the Campylobacteraceae strains have these putative virulence genes that may lead to pathogenic effects in humans.

A characterization methodology for post-wildfire flood hazard assessments

DOE Office of Scientific and Technical Information (OSTI.GOV)

McLin, Stephen G.; Van Eeckhout, M. E.; Springer, E. P.

A combined GIS-HEC modeling application for floodplain analysis of pre- and post-burned watersheds is described. The burned study area is located on Pajarito Plateau near Los Alamos, New Mexico (USA), where the Cerro Grande Wildfire burned 17,353 ha (42,878 ac) in May 2000. This area is dominated by rugged mountains that are dissected by numerous steep canyons having both ephemeral and perennial channel reaches. Vegetation consists of pinon-juniper woodlands located between 1,829-2,134 m (6,000-7,000 ft) above mean sea level (m MSL), and Ponderosa pine stands between 2,134-3,048 m MSL (7,000-10,000 ft). Approximately seventeen percent of the burned area is locatedmore » within Los Alamos National Laboratory, and the remainder is located in upstream or adjacent watersheds. Pre-burn floodplains were previously mapped in 1991-92 using early HEC models as part of the RCRA/HSWA permitting process. Numerous recording precipitation and stream gages have also been installed. These data provide essential information characterizing rainfall-runoff relationships before and after the fire. They are also being used to monitor spatial and temporal changes as forest recovery progresses. Post-burn changes in HEC-HMS predicted rainfall-runoff patterns are related to changes in watershed vegetation cover and hydrophobic soil conditions. Stream channel cross-sectional geometries were extracted from 0.3 m (1 ft) DEM data using ArcView GIS. Then floodpool topwidths, depths, and flow velocities were remapped using the HEC-RAS model. Finally, numerous surveyed channel sections were selectively made at crucial sites for model verification. Direct comparisons are made between alternative data acquisition and mapping techniques.« less

Evaluation of pier-scour equations for coarse-bed streams

USGS Publications Warehouse

Chase, Katherine J.; Holnbeck, Stephen R.

2004-01-01

Streambed scour at bridge piers is among the leading causes of bridge failure in the United States. Several pier-scour equations have been developed to calculate potential scour depths at existing and proposed bridges. Because many pier-scour equations are based on data from laboratory flumes and from cohesionless silt- and sand-bottomed streams, they tend to overestimate scour for piers in coarse-bed materials. Several equations have been developed to incorporate the mitigating effects of large particle sizes on pier scour, but further investigations are needed to evaluate how accurately pier-scour depths calculated by these equations match measured field data. This report, prepared in cooperation with the Montana Department of Transportation, describes the evaluation of five pier-scour equations for coarse-bed streams. Pier-scour and associated bridge-geometry, bed-material, and streamflow-measurement data at bridges over coarse-bed streams in Montana, Alaska, Maryland, Ohio, and Virginia were selected from the Bridge Scour Data Management System. Pier scour calculated using the Simplified Chinese equation, the Froehlich equation, the Froehlich design equation, the HEC-18/Jones equation and the HEC-18/Mueller equation for flood events with approximate recurrence intervals of less than 2 to 100 years were compared to 42 pier-scour measurements. Comparison of results showed that pier-scour depths calculated with the HEC-18/Mueller equation were seldom smaller than measured pier-scour depths. In addition, pier-scour depths calculated using the HEC-18/Mueller equation were closer to measured scour than for the other equations that did not underestimate pier scour. However, more data are needed from coarse-bed streams and from less frequent flood events to further evaluate pier-scour equations.

Helium isotope, C/3He, and Ba-Nb-Ti signatures in the northern Lau Basin: Distinguishing arc, back-arc, and hotspot affinities

NASA Astrophysics Data System (ADS)

Lupton, John; Rubin, Ken H.; Arculus, Richard; Lilley, Marvin; Butterfield, David; Resing, Joseph; Baker, Edward; Embley, Robert

2015-04-01

The northern Lau Basin hosts a complicated pattern of volcanism, including Tofua Arc volcanoes, several back-arc spreading centers, and individual "rear-arc" volcanoes not associated with these structures. Elevated 3He/4He ratios in lavas of the NW Lau Spreading Center suggest the influence of a mantle plume, possibly from Samoa. We show that lavas from mid-ocean ridges, volcanic arcs, and hotspots occupy distinct, nonoverlapping fields in a 3He/4He versus C/3He plot. Applied to the northern Lau Basin, this approach shows that most of Lau back-arc spreading systems have mid-ocean ridge 3He/4He-C/3He characteristics, except the NW Lau spreading center, which has 3He/4He-C/3He similar to "high 3He" hotspots such as Loihi, Kilauea, and Yellowstone, but with slightly lower C/3He. Niua seamount, on the northern extension of the Tofua Arc, falls squarely in the arc field. All the NE Lau rear-arc volcanoes, including the recently erupting West Mata, also have arc-like 3He/4He-C/3He characteristics. Ba-Nb-Ti contents of the lavas, which are more traditional trace element indicators of mantle source enrichment, depletion, and subduction input, likewise indicate arc and hot spot influences in the lavas of the northern Lau Basin, but in a more ambiguous fashion because of a complex prior history. This verifies that 3He/4He-C/3He systematics are useful for differentiating between mid-ocean ridge, arc, and hotspot affinities in submarine volcanic systems, that all three of these affinities are expressed in the northern Lau Basin, and provides additional support for the Samoan plume influence in the region.

Integration of contributed data with HEC-RAS hydrodynamic model for flood inundation and damage assessment: 2015 Dallas Texas Case Study

NASA Astrophysics Data System (ADS)

Sava, E.; Thornton, J. C.; Kalyanapu, A. J.; Cervone, G.

2016-12-01

Transportation infrastructure networks in urban areas are highly sensitive to natural disasters, yet are a very critical source for the success of rescue, recovery, and renovation operations. Therefore, prompt restoration of such networks is of high importance for disaster relief services. Satellite and aerial images provide data with high spatial and temporal resolution and are a powerful tool for monitoring the environment and mapping the spatio-temporal variability of the Earth's surface. They provide a synoptic overview and give useful environmental information for a wide range of scales, from entire continents to urban areas, with spatial pixel resolutions ranging from kilometers to centimeters. However, sensor limitations are often a serious drawback since no single sensor offers the optimal spectral, spatial, and temporal resolution at the same time. Specific data may not be collected in the time and space most urgently required and/or may it contain gaps as a result of the satellite revisit time, atmospheric opacity, or other obstructions. In this study, the feasibility of integrating multiple sources of contributed data including remotely sensed datasets and open-source geospatial datasets, into hydrodynamic models for flood inundation simulations is assessed. The 2015 Dallas floods that caused up to $61 million dollars in damage was selected for this study. A Hydraulic Engineering Center - River Analysis System (HEC-RAS) model was developed for the study area, using reservoir surcharge releases and geometry provided by the U.S. Army Corps of Engineers Fort Worth District. The simulated flood inundation is compared with the "contributed data" for the location (such as Civil Air Patrol data and WorldView 3 dataset) which indicated the model's lack of representing lateral inflows near the upstream section. An Artificial Neural Network (ANN) model is developed that used local precipitation and discharge values in the vicinity to estimate the lateral flows. This addition of estimated lateral inflows is expected to improve the model performance to match with the observed flows. Future work will focus on extending this preliminary work to assess the model performance after integrating these additional data sources.

Miniature Pulse Tube Cooler

NASA Astrophysics Data System (ADS)

Tward, E.; Nguyen, T.; Godden, J.; Toma, G.

2004-06-01

A high capacity miniature pulse tube cooler for space that is scaled from the High Efficiency Cryocooler (HEC) is being developed. The low mass (1.5 kg) integral pulse tube cryocooler can provide large cooling power over a wide temperature range (e.g., 5 W at 95 K). The cooler is designed to be compatible with the existing HEC flight electronics. A small back-to-back flexure compressor drives a pulse tube cold head which is integrated with the compressor. The cooler has been tested with both linear and coaxial cold heads. A description of the cooler and its performance in both linear and coaxial cold head versions is presented.

Helmet Exhalation Capture System (HECS) Sizing Evaluation for an Advanced Space Suit Portable Life Support System

NASA Technical Reports Server (NTRS)

Paul, Heather L.; Waguespack, Glenn M.; Paul, Thomas H.; Conger, Bruce C.

2008-01-01

As part of NASA s initiative to develop an advanced portable life support system (PLSS), a baseline schematic has been chosen that includes gaseous oxygen in a closed circuit ventilation configuration. Supply oxygen enters the suit at the back of the helmet and return gases pass over the astronaut s body to be extracted at the astronaut s wrists and ankles through the liquid cooling and ventilation garment (LCVG). The extracted gases are then treated using a rapid cycling amine (RCA) system for carbon dioxide and water removal and activated carbon for trace gas removal before being mixed with makeup oxygen and reintroduced into the helmet. Thermal control is provided by a suit water membrane evaporator (SWME). As an extension of the original schematic development, NASA evaluated several Helmet Exhalation Capture System (HECS) configurations as alternatives to the baseline. The HECS configurations incorporate the use of full contact masks or non-contact masks to reduce flow requirements within the PLSS ventilation subsystem. The primary scope of this study was to compare the alternatives based on mass and volume considerations; however other design issues were also briefly investigated. This paper summarizes the results of this sizing analysis task.

The basilar membrane acts as a passive support structure at the cochlear apex

NASA Astrophysics Data System (ADS)

Warren, Rebecca L.; Fridberger, Anders

2015-12-01

The precise mechanical behavior of the basilar membrane (BM) at low frequencies is still unknown. To address this issue we use an in vitro preparation of the guinea pig temporal bone to investigate the mechanical behaviour of the organ of Corti at the apex of the cochlea. Confocal laser interferometry is used to record the nanometre displacements of both Hensen's cells (HeC) and the BM in response to sound and electrical stimulation. We show that at low frequencies, the BM exhibits greatly reduced sound-evoked movement (˜35dB less) and no current-evoked movement, when compared to the HeC at the same position along the spiral. The BM best frequency is found to be an average of 52Hz (0.35 octave) higher than the HeC best frequency. In addition, we demonstrate that BM motion is not affected by inhibition of somatic electromotility or by blocking the mechanoelectrical transduction channels.We therefore propose that the BM primarily acts as a passive support structure at the cochlear apex. We suggest that the micromechanics of the cochlea that are vital to low-frequency amplification and frequency selectivity take place predominantly at the surface of the organ of Corti.

Rolapitant: A Review in Chemotherapy-Induced Nausea and Vomiting.

PubMed

Heo, Young-A; Deeks, Emma D

2017-10-01

Oral rolapitant (Varubi™; Varuby ® ), a long-acting neurokinin-1 (NK 1 ) receptor antagonist (RA), is indicated in the USA and EU as part of an antiemetic regimen to prevent delayed chemotherapy-induced nausea and vomiting (CINV) in adults receiving highly or moderately emetogenic chemotherapy (HEC or MEC). In randomized, phase III trials, a single oral dose of rolapitant 180 mg was effective in preventing delayed CINV compared with placebo, when each was used in combination with a 5-HT 3 RA plus dexamethasone, in adults receiving their first course of HEC or MEC. The benefits of rolapitant were maintained over multiple cycles of chemotherapy. The tolerability profile of rolapitant is similar to that of placebo and consistent with that of other NK 1 RAs. However, rolapitant differs from other existing NK 1 RAs in that it does not interact with CYP3A4, thereby negating the need for dexamethasone dose adjustments and potentially making rolapitant a more suitable option for patients receiving CYP3A4 substrates. Thus, oral rolapitant is an effective and well tolerated NK 1 RA that expands the treatment options for preventing delayed CINV in adults receiving HEC or MEC.

The Cumberland River Flood of 2010 and Corps Reservoir Operations

NASA Astrophysics Data System (ADS)

Charley, W.; Hanbali, F.; Rohrbach, B.

2010-12-01

On Saturday, May 1, 2010, heavy rain began falling in the Cumberland River Valley and continued through the following day. 13.5 inches was measured at Nashville, an unprecedented amount that doubled the previous 2-day record, and exceeded the May monthly total record of 11 inches. Elsewhere in the valley, amounts of over 19 inches were measured. The frequency of this storm was estimated to exceed the one-thousand year event. This historic rainfall brought large scale flooding to the Cumberland-Ohio-Tennessee River Valleys, and caused over 2 billion dollars in damages, despite the numerous flood control projects in the area, including eight U.S. Army Corps of Engineers projects. The vast majority of rainfall occurred in drainage areas that are uncontrolled by Corps flood control projects, which lead to the wide area flooding. However, preliminary analysis indicates that operations of the Corps projects reduced the Cumberland River flood crest in Nashville by approximately five feet. With funding from the American Recovery and Reinvestment Act (ARRA) of 2009, hydrologic, hydraulic and reservoir simulation models have just been completed for the Cumberland-Ohio-Tennessee River Valleys. These models are being implemented in the Corps Water Management System (CWMS), a comprehensive data acquisition and hydrologic modeling system for short-term decision support of water control operations in real time. The CWMS modeling component uses observed rainfall and forecasted rainfall to compute forecasts of river flows into and downstream of reservoirs, using HEC-HMS. Simulation of reservoir operations, utilizing either the HEC-ResSim or CADSWES RiverWare program, uses these flow scenarios to provide operational decision information for the engineer. The river hydraulics program, HEC-RAS, computes river stages and water surface profiles for these scenarios. An inundation boundary and depth map of water in the flood plain can be calculated from the HEC-RAS results using ArcInfo. The economic impacts of the different inundation depths are computed by HEC-FIA. The user-configurable sequence of modeling software allows engineers to evaluate operational decisions for reservoirs and other control structures, and view and compare hydraulic and economic impacts for various “what if?” scenarios. This paper reviews the Cumberland River May 2010 event, the impact of Corps reservoirs and reservoir operations and the expected future benefits and effects of the ARRA funded models and CWMS on future events for this area.

Experimental Investigation and Analysis of HEC-6 River Morphological Model

NASA Astrophysics Data System (ADS)

Tingsanchali, Tawatchai; Supharatid, Seree

1996-05-01

Only comparatively few experimental studies have been carried out to investigate the performance of the HEC-6 river morphological model. The model was developed by the Hydrologic Engineering Center of the US Army Corps of Engineers. In this study, experiments were carried out in a 20 m long concrete flume 0.6 m wide with varying rectangular cross-sections. The channel bed is paved with uniform sand of D50 = 0.9 mm and D90 = 1.2 mm within the test reach of 12 m. Two types of experiments were carried out with sediment transport, one under steady uniform flow and another under steady non-uniform flow conditions. Nine steady uniform flow experiments were carried out to compare the measured equilibrium relationship of flow and sediment transport rate with two bedload formulae, namely, Du Boys and Meyer-Peter and Muller, and with three total load formulae, namely, Toffaleti, Laursen and Yang. It was found that even though the sediment transport consists of a certain portion of bedload, the total load formulae give satisfactory results and better agreement than the two bedload formulae. Five steady non-uniform flow experiments were carried out under various conditions of varying bed profile and channel width and also with sediment addition and withdrawal. The measured transient water surface and bed profiles are compared with the computed results from the HEC-6 model. It was found that the Toffaleti and Yang total load formulae used in the HEC-6 model give the most satisfactory prediction of actual bed profiles under various conditions of non-uniform flow and sediment transport. The effects of Manning's n, variations of sediment inflow, various sediment transport formulae, sediment grain size and the model numerical parameters, i.e. distance interval x and numerical weighting factor, on the computed water surface and bed profiles were determined. It was found that the selection of the sediment transport formulae has the most significant effect on the computed results. It can be concluded that the HEC-6 model can predict satisfactorily a long-term average pattern of local scour and deposition along a channel with either a small abrupt change in geometry or gradually varying cross-sections. However, the accuracy of the model prediction is reduced in the regions where highly non-uniform flow occurs.

Experiments with Interaction between the National Water Model and the Reservoir System Simulation Model: A Case Study of Russian River Basin

NASA Astrophysics Data System (ADS)

Kim, J.; Johnson, L.; Cifelli, R.; Chandra, C. V.; Gochis, D.; McCreight, J. L.; Yates, D. N.; Read, L.; Flowers, T.; Cosgrove, B.

2017-12-01

NOAA National Water Center (NWC) in partnership with the National Centers for Environmental Prediction (NCEP), the National Center for Atmospheric Research (NCAR) and other academic partners have produced operational hydrologic predictions for the nation using a new National Water Model (NWM) that is based on the community WRF-Hydro modeling system since the summer of 2016 (Gochis et al., 2015). The NWM produces a variety of hydrologic analysis and prediction products, including gridded fields of soil moisture, snowpack, shallow groundwater levels, inundated area depths, evapotranspiration as well as estimates of river flow and velocity for approximately 2.7 million river reaches. Also included in the NWM are representations for more than 1,200 reservoirs which are linked into the national channel network defined by the USGS NHDPlusv2.0 hydrography dataset. Despite the unprecedented spatial and temporal coverage of the NWM, many known deficiencies exist, including the representation of lakes and reservoirs. This study addresses the implementation of a reservoir assimilation scheme through coupling of a reservoir simulation model to represent the influence of managed flows. We examine the use of the reservoir operations to dynamically update lake/reservoir storage volume states, characterize flow characteristics of river reaches flowing into and out of lakes and reservoirs, and incorporate enhanced reservoir operating rules for the reservoir model options within the NWM. Model experiments focus on a pilot reservoir domain-Lake Mendocino, CA, and its contributing watershed, the East Fork Russian River. This reservoir is modeled using United States Army Corps of Engineers (USACE) HEC-ResSim developed for application to examine forecast informed reservoir operations (FIRO) in the Russian River basin.

A randomized control trial to evaluate the importance of pre-training basic laparoscopic psychomotor skills upon the learning curve of laparoscopic intra-corporeal knot tying.

PubMed

Molinas, Carlos Roger; Binda, Maria Mercedes; Sisa, Cesar Manuel; Campo, Rudi

2017-01-01

Training of basic laparoscopic psychomotor skills improves the acquisition of more advanced laparoscopic tasks, such as laparoscopic intra-corporeal knot tying (LICK). This randomized controlled trial was designed to evaluate whether pre-training of basic skills, as laparoscopic camera navigation (LCN), hand-eye coordination (HEC), and bimanual coordination (BMC), and the combination of the three of them, has any beneficial effect upon the learning curve of LICK. The study was carried out in a private center in Asunción, Paraguay, by 80 medical students without any experience in surgery. Four laparoscopic tasks were performed in the ENCILAP model (LCN, HEC, BMC, and LICK). Participants were allocated to 5 groups (G1-G5). The study was structured in 5 phases. In phase 1, they underwent a base-line test ( T 1 ) for all tasks (1 repetition of each task in consecutive order). In phase 2, participants underwent different training programs (30 consecutive repetitions) for basic tasks according to the group they belong to (G1: none; G2: LCN; G3: HEC; G4: BMC; and G5: LCN, HEC, and BMC). In phase 3, they were tested again ( T 2 ) in the same manner than at T 1 . In phase 4, they underwent a standardized training program for LICK (30 consecutive repetitions). In phase 5, they were tested again ( T 3 ) in the same manner than at T 1 and T 2 . At each repetition, scoring was based on the time taken for task completion system. The scores were plotted and non-linear regression models were used to fit the learning curves to one- and two-phase exponential decay models for each participant (individual curves) and for each group (group curves). The LICK group learning curves fitted better to the two-phase exponential decay model. From these curves, the starting points ( Y 0), the point after HEC training/before LICK training ( Y 1), the Plateau, and the rate constants ( K ) were calculated. All groups, except for G4, started from a similar point ( Y 0). At Y 1, G5 scored already better than the others (G1 p  = .004; G2 p  = .04; G3 p  < .0001; G4 NS). Although all groups reached a similar Plateau, G5 has a quicker learning than the others, demonstrated by a higher K (G1 p  < 0.0001; G2 p  < 0.0001; G3 p  < 0.0001; and G4 p  < 0.0001). Our data confirms that training improves laparoscopic skills and demonstrates that pre-training of all basic skills (i.e., LCN, HEC, and BMC) shortens the LICK learning curve.

The effect of replacing pnictogen elements on the physical properties SrMg2 {{\\boldsymbol{X}}}_{2} ({\\boldsymbol{X}}=N, P, As, Sb, Bi) Zintl compounds

NASA Astrophysics Data System (ADS)

Murtaza, G.; Ahad Khan, Abdul; Yaseen, M.; Laref, A.; Ullah, Naeem; Rahman, Inayat ur

2018-04-01

Not Available Project supported by a grant from the “Research Center of Female Scientific and Medical Colleges”, the Deanship of Scientific Research, King Saud University. Yaseen M is thankful to Higher Education Commission (HEC), Pakistan for funding (Grant No. 6410/Punjab/NRPU/R&D/HEC/2016), and also this study is made possible by the support of the United States Government and the American people through the United States Agency for International Development (USAID). The contents are the sole responsibility of National University of Sciences and Technology and do not necessarily reflect the views of USAID or the United States Government.

Role of High-End Computing in Meeting NASA's Science and Engineering Challenges

NASA Technical Reports Server (NTRS)

Biswas, Rupak

2006-01-01

High-End Computing (HEC) has always played a major role in meeting the modeling and simulation needs of various NASA missions. With NASA's newest 62 teraflops Columbia supercomputer, HEC is having an even greater impact within the Agency and beyond. Significant cutting-edge science and engineering simulations in the areas of space exploration, Shuttle operations, Earth sciences, and aeronautics research, are already occurring on Columbia, demonstrating its ability to accelerate NASA s exploration vision. The talk will describe how the integrated supercomputing production environment is being used to reduce design cycle time, accelerate scientific discovery, conduct parametric analysis of multiple scenarios, and enhance safety during the life cycle of NASA missions.

Nonstationarities in Catchment Response According to Basin and Rainfall Characteristics: Application to Korean Watershed

NASA Astrophysics Data System (ADS)

Kwon, Hyun-Han; Kim, Jin-Guk; Jung, Il-Won

2015-04-01

It must be acknowledged that application of rainfall-runoff models to simulate rainfall-runoff processes are successful in gauged watershed. However, there still remain some issues that will need to be further discussed. In particular, the quantitive representation of nonstationarity issue in basin response (e.g. concentration time, storage coefficient and roughness) along with ungauged watershed needs to be studied. In this regard, this study aims to investigate nonstationarity in basin response so as to potentially provide useful information in simulating runoff processes in ungauged watershed. For this purpose, HEC-1 rainfall-runoff model was mainly utilized. In addition, this study combined HEC-1 model with Bayesian statistical model to estimate uncertainty of the parameters which is called Bayesian HEC-1 (BHEC-1). The proposed rainfall-runofall model is applied to various catchments along with various rainfall patterns to understand nonstationarities in catchment response. Further discussion about the nonstationarity in catchment response and possible regionalization of the parameters for ungauged watershed are discussed. KEYWORDS: Nonstationary, Catchment response, Uncertainty, Bayesian Acknowledgement This research was supported by a Grant (13SCIPA01) from Smart Civil Infrastructure Research Program funded by the Ministry of Land, Infrastructure and Transport (MOLIT) of Korea government and the Korea Agency for Infrastructure Technology Advancement (KAIA).

Numerical Analysis of Flood modeling of upper Citarum River under Extreme Flood Condition

NASA Astrophysics Data System (ADS)

Siregar, R. I.

2018-02-01

This paper focuses on how to approach the numerical method and computation to analyse flood parameters. Water level and flood discharge are the flood parameters solved by numerical methods approach. Numerical method performed on this paper for unsteady flow conditions have strengths and weaknesses, among others easily applied to the following cases in which the boundary irregular flow. The study area is in upper Citarum Watershed, Bandung, West Java. This paper uses computation approach with Force2 programming and HEC-RAS to solve the flow problem in upper Citarum River, to investigate and forecast extreme flood condition. Numerical analysis based on extreme flood events that have occurred in the upper Citarum watershed. The result of water level parameter modeling and extreme flood discharge compared with measurement data to analyse validation. The inundation area about flood that happened in 2010 is about 75.26 square kilometres. Comparing two-method show that the FEM analysis with Force2 programs has the best approach to validation data with Nash Index is 0.84 and HEC-RAS that is 0.76 for water level. For discharge data Nash Index obtained the result analysis use Force2 is 0.80 and with use HEC-RAS is 0.79.

Incorporation of dosimetry in the derivation of reference concentrations for ambient or workplace air: a conceptual approach.

PubMed

Oller, Adriana R; Oberdörster, Günter

2016-09-01

Dosimetric models are essential tools to refine inhalation risk assessments based on local respiratory effects. Dosimetric adjustments to account for differences in aerosol particle size and respiratory tract deposition and/or clearance among rodents, workers, and the general public can be applied to experimentally- and epidemiologically-determined points of departure (PODs) to calculate size-selected (e.g., PM 10 , inhalable aerosol fraction, respirable aerosol fraction) equivalent concentrations (e.g., HEC or Human Equivalent Concentration; REC or Rodent Equivalent Concentration). A modified POD (e.g., HEC) can then feed into existing frameworks for the derivation of occupational or ambient air concentration limits or reference concentrations. HECs that are expressed in terms of aerosol particle sizes experienced by humans but are derived from animal studies allow proper comparison of exposure levels and associated health effects in animals and humans. This can inform differences in responsiveness between animals and humans, based on the same deposited or retained doses and can also allow the use of both data sources in an integrated weight of evidence approach for hazard and risk assessment purposes. Whenever possible, default values should be replaced by substance-specific and target population-specific parameters. Assumptions and sources of uncertainty need to be clearly reported.

Incorporation of dosimetry in the derivation of reference concentrations for ambient or workplace air: a conceptual approach

PubMed Central

Oberdörster, Günter

2016-01-01

Dosimetric models are essential tools to refine inhalation risk assessments based on local respiratory effects. Dosimetric adjustments to account for differences in aerosol particle size and respiratory tract deposition and/or clearance among rodents, workers, and the general public can be applied to experimentally- and epidemiologically-determined points of departure (PODs) to calculate size-selected (e.g., PM10, inhalable aerosol fraction, respirable aerosol fraction) equivalent concentrations (e.g., HEC or Human Equivalent Concentration; REC or Rodent Equivalent Concentration). A modified POD (e.g., HEC) can then feed into existing frameworks for the derivation of occupational or ambient air concentration limits or reference concentrations. HECs that are expressed in terms of aerosol particle sizes experienced by humans but are derived from animal studies allow proper comparison of exposure levels and associated health effects in animals and humans. This can inform differences in responsiveness between animals and humans, based on the same deposited or retained doses and can also allow the use of both data sources in an integrated weight of evidence approach for hazard and risk assessment purposes. Whenever possible, default values should be replaced by substance-specific and target population-specific parameters. Assumptions and sources of uncertainty need to be clearly reported. PMID:27721518

From reactive to proactive: developing a valid clinical ethics needs assessment survey to support ethics program strategic planning (part 1 of 2).

PubMed

Frolic, Andrea; Jennings, Barb; Seidlitz, Wendy; Andreychuk, Sandy; Djuric-Paulin, Angela; Flaherty, Barb; Peace, Donna

2013-03-01

As ethics committees and programs become integrated into the "usual business" of healthcare organizations, they are likely to face the predicament of responding to greater demands for service and higher expectations, without an influx of additional resources. This situation demands that ethics committees and programs allocate their scarce resources (including their time, skills and funds) strategically, rather than lurching from one ad hoc request to another; finding ways to maximize the effectiveness, efficiency, impact and quality of ethics services is essential in today's competitive environment. How can Hospital Ethics Committees (HECs) begin the process of strategic priority-setting to ensure they are delivering services where and how they are most needed? This paper describes the creation of the Clinical Ethics Needs Assessment Survey (CENAS) as a tool to understand interprofessional staff perceptions of the organization's ethical climate, challenging ethical issues and educational priorities. The CENAS was designed to support informed resource allocation and advocacy by HECs. By sharing our process of developing and validating this ethics needs assessment survey we hope to enable strategic priority-setting in other resource-strapped ethics programs, and to empower HECs to shift their focus to more proactive, quality-focused initiatives.

Ethanol production using immobilized Saccharomyces cerevisiae in lyophilized cellulose gel.

PubMed

Winkelhausen, Eleonora; Velickova, Elena; Amartey, Samuel A; Kuzmanova, Slobodanka

2010-12-01

A new lyophilization technique was used for immobilization of Saccharomyces cerevisiae cells in hydroxyethylcellulose (HEC) gels. The suitability of the lyophilized HEC gels to serve as immobilization matrices for the yeast cells was assessed by calculating the immobilization efficiency and the cell retention in three consecutive batches, each in duration of 72 h. Throughout the repeated batch fermentation, the immobilization efficiency was almost constant with an average value of 0.92 (12-216 h). The maximum value of cell retention was 0.24 g immobilized cells/g gel. Both parameters indicated that lyophilized gels are stable and capable of retaining the immobilized yeast cells. Showing the yeast cells propagation within the polymeric matrix, the scanning electron microscope images also confirmed that the lyophilization technique for immobilization of S. cerevisiae cells in the HEC gels was successful. The activity of the immobilized yeast cells was demonstrated by their capacity to convert glucose to ethanol. Ethanol yield of 0.40, 0.43 and 0.30 g ethanol/g glucose corresponding to 79%, 84% and 60% of the theoretical yield was attained in the first, second and third batches, respectively. The cell leakage was less than 10% of the average concentration of the immobilized cells.

Clay Nanoparticles Elicit Long-Term Immune Responses by Forming Biodegradable Depots for Sustained Antigen Stimulation.

PubMed

Chen, Weiyu; Zuo, Huali; Li, Bei; Duan, Chengcheng; Rolfe, Barbara; Zhang, Bing; Mahony, Timothy J; Xu, Zhi Ping

2018-05-01

Nanomaterials have been widely tested as new generation vaccine adjuvants, but few evoke efficient immunoreactions. Clay nanoparticles, for example, layered double hydroxide (LDH) and hectorite (HEC) nanoparticles, have shown their potent adjuvanticity in generating effective and durable immune responses. However, the mechanism by which clay nanoadjuvants stimulate the immune system is not well understood. Here, it is demonstrated that LDH and HEC-antigen complexes form loose agglomerates in culture medium/serum. They also form nodules with loose structures in tissue after subcutaneous injection, where they act as a depot for up to 35 d. More importantly, clay nanoparticles actively and continuously recruit immune cells into the depot for up to one month, and stimulate stronger immune responses than FDA-approved adjuvants, Alum and QuilA. Sustained antigen release is also observed in clay nanoparticle depots, with 50-60% antigen released after 35 d. In contrast, Alum-antigen complexes show minimal antigen release from the depot. Importantly, LDH and HEC are more effective than QuilA and Alum in promoting memory T-cell proliferation. These findings suggest that both clay nanoadjuvants can serve as active vaccine platforms for sustained and potent immune responses. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

An Operational Short-Term Forecasting System for Regional Hydropower Management

NASA Astrophysics Data System (ADS)

Gronewold, A.; Labuhn, K. A.; Calappi, T. J.; MacNeil, A.

2017-12-01

The Niagara River is the natural outlet of Lake Erie and drains four of the five Great lakes. The river is used to move commerce and is home to both sport fishing and tourism industries. It also provides nearly 5 million kilowatts of hydropower for approximately 3.9 million homes. Due to a complex international treaty and the necessity of balancing water needs for an extensive tourism industry, the power entities operating on the river require detailed and accurate short-term river flow forecasts to maximize power output. A new forecast system is being evaluated that takes advantage of several previously independent components including the NOAA Lake Erie operational Forecast System (LEOFS), a previously developed HEC-RAS model, input from the New York Power Authority(NYPA) and Ontario Power Generation (OPG) and lateral flow forecasts for some of the tributaries provided by the NOAA Northeast River Forecast Center (NERFC). The Corps of Engineers updated the HEC-RAS model of the upper Niagara River to use the output forcing from LEOFS and a planned Grass Island Pool elevation provided by the power entities. The entire system has been integrated at the NERFC; it will be run multiple times per day with results provided to the Niagara River Control Center operators. The new model helps improve discharge forecasts by better accounting for dynamic conditions on Lake Erie. LEOFS captures seiche events on the lake that are often several meters of displacement from still water level. These seiche events translate into flow spikes that HEC-RAS routes downstream. Knowledge of the peak arrival time helps improve operational decisions at the Grass Island Pool. This poster will compare and contrast results from the existing operational flow forecast and the new integrated LEOFS/HEC-RAS forecast. This additional model will supply the Niagara River Control Center operators with multiple forecasts of flow to help improve forecasting under a wider variety of conditions.

Evaluation of in vitro anticancer activity of 1,8-Cineole-containing n-hexane extract of Callistemon citrinus (Curtis) Skeels plant and its apoptotic potential.

PubMed

Sampath, Sowndarya; Veeramani, Vidhya; Krishnakumar, Gopal Shankar; Sivalingam, Udhayakumar; Madurai, Suguna Lakshmi; Chellan, Rose

2017-09-01

Plants are the source of a variety of secondary metabolites, which are often used in the anticancer activity. Discovering new anticancer drug from herbal source is more important in both biological and pharmacological activities. Hence, the objective of this study is to identify the anticancer agent in Callistemon citrinus (Curtis) Skeels (CC) for the treatment of cancer. Very recently we have reported an increased antioxidant activity in the ethanolic and methanolic extracts (EE and ME) of CC but significantly reduced activity (rather increased cytotoxicity), in the n-hexane extract (HE). In this study, the cytotoxicity of all the three solvent extracts was tested against A431, MG-63 and HaCaT cell lines by MTT assay. Interestingly HE has showed increased anti-proliferative effect against the cancer cells but was resisted by non-malignant cells. HPLC and GC-MS analysis revealed the presence of 1,8-Cineole as a predominant compound in HE, the semi-purified bioactive extract. Henceforth, this would be called HE-C and be used for further analyses to understand its mode of action on induced apoptosis/necrosis. Alamar blue assay of HE-C showed cytotoxicity and change in morphological characteristics, which was confirmed by AO/EB staining using fluorescence microscopy, ultra-structural features of apoptosis using SEM and TEM. HE-C induced cell death was also detected by FACS using FITC-labelled Annexin-V and Propidium iodide. ROS generation was monitored using DCF-DA by flow cytometry. The overall results suggested that the selective extract (HE-C) containing 1,8-Cineole has shown potential anti-cancer activity in a dose-dependent manner, and cell death was induced through ROS-mediated apoptosis. Our findings provide an insight into the potential of 1,8-Cineole as a novel drug for killing cancer cells. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

Swelling/Floating Capability and Drug Release Characterizations of Gastroretentive Drug Delivery System Based on a Combination of Hydroxyethyl Cellulose and Sodium Carboxymethyl Cellulose

PubMed Central

Chen, Ying-Chen; Ho, Hsiu-O; Liu, Der-Zen; Siow, Wen-Shian; Sheu, Ming-Thau

2015-01-01

The aim of this study was to characterize the swelling and floating behaviors of gastroretentive drug delivery system (GRDDS) composed of hydroxyethyl cellulose (HEC) and sodium carboxymethyl cellulose (NaCMC) and to optimize HEC/NaCMC GRDDS to incorporate three model drugs with different solubilities (metformin, ciprofloxacin, and esomeprazole). Various ratios of NaCMC to HEC were formulated, and their swelling and floating behaviors were characterized. Influences of media containing various NaCl concentrations on the swelling and floating behaviors and drug solubility were also characterized. Finally, release profiles of the three model drugs from GRDDS formulation (F1-4) and formulation (F1-1) were examined. Results demonstrated when the GRDDS tablets were tested in simulated gastric solution, the degree of swelling at 6 h was decreased for each formulation that contained NaCMC in comparison to those in de-ionized water (DIW). Of note, floating duration was enhanced when in simulated gastric solution compared to DIW. Further, the hydration of tablets was found to be retarded as the NaCl concentration in the medium increased resulting in smaller gel layers and swelling sizes. Dissolution profiles of the three model drugs in media containing various concentrations of NaCl showed that the addition of NaCl to the media affected the solubility of the drugs, and also their gelling behaviors, resulting in different mechanisms for controlling a drug’s release. The release mechanism of the freely water-soluble drug, metformin, was mainly diffusion-controlled, while those of the water-soluble drug, ciprofloxacin, and the slightly water-soluble drug, esomeprazole, were mainly anomalous diffusion. Overall results showed that the developed GRDDS composed of HEC 250HHX and NaCMC of 450 cps possessed proper swelling extents and desired floating periods with sustained-release characteristics. PMID:25617891

Predicting floodplain boundary changes following the Cerro Grande wildfire

NASA Astrophysics Data System (ADS)

McLin, Stephen G.; Springer, Everett P.; Lane, Leonard J.

2001-10-01

A combined ArcView GIS-HEC modelling application for floodplain analysis of pre- and post-burned watersheds is described. The burned study area is located on Pajarito Plateau near Los Alamos National Laboratory (the Laboratory), where the Cerro Grande Wildfire burned 42 878 acres (17 352 ha) in May 2000. This area is dominated by rugged mountains that are dissected by numerous steep canyons having both ephemeral and perennial channel reaches. Vegetation consists of pinon-juniper woodlands located between 6000 and 7000 ft (1829-2134 m) above mean sea level (MSL), and Ponderosa pine stands between 7000 and 10000 ft MSL (2134-3048 m). Approximately 17% of the burned area is located within the Laboratory, and the remainder is located in upstream or adjacent watersheds. Pre-burn floodplains were previously mapped in 1990-91 using early HEC models as part of the hazardous waste site permitting process. Precipitation and stream gauge data provide essential information characterizing rainfall-runoff relationships before and after the fire. They also provide a means of monitoring spatial and temporal changes as forest recovery progresses. The 2000 summer monsoon began in late June and provided several significant runoff events for model calibration. HEC-HMS modelled responses were sequentially refined so that observed and predicted hydrograph peaks were matched at numerous channel locations. The 100 year, 6 h design storm was eventually used to predict peak hydrographs at critical sites. These results were compared with pre-fire simulations so that new flood-prone areas could be systematically identified. Stream channel cross-sectional geometries were extracted from a gridded 1 ft (0·3 m) digital elevation model (DEM) using ArcView GIS. Then floodpool topwidths, depths, and flow velocities were remapped using the HEC-RAS model. Finally, numerous surveyed channel sections were selectively made at crucial sites for DEM verification. These evaluations provided timely guidance that influenced the decision to construct several flood detention structures that were completed in September 2000. Published in 2001 John Wiley & Sons, Ltd.

Enteropathogen infections in canine puppies: (Co-)occurrence, clinical relevance and risk factors.

PubMed

Duijvestijn, Mirjam; Mughini-Gras, Lapo; Schuurman, Nancy; Schijf, Wim; Wagenaar, Jaap A; Egberink, Herman

2016-11-15

Laboratory confirmation of the causative agent(s) of diarrhoea in puppies may allow for appropriate treatment. The presence of potential pathogens however, does not prove a causal relationship with diarrhoea. The aim of this study was to identify specific enteropathogens in ≤12 month old puppies with and without acute diarrhoea and to assess their associations with clinical signs, putative risk factors and pathogen co-occurrence. Faecal samples from puppies with (n=113) and without (n=56) acute diarrhoea were collected and screened for Canine Parvovirus (CPV), Canine Coronavirus (CCoV), Salmonella spp., Campylobacter spp., Clostridium perfringens, Clostridium difficile, β-hemolytic Eschericha coli (hEC), Giardia spp., Toxocara spp., Cystoisospora spp., and Cyniclomyces guttulatus. One or more pathogens were detected in 86.5% of diarrhoeic puppies and in 77.8% of asymptomatic puppies. Significant positive associations were found between CPV and CCoV, CPV and Cystoisospora spp., Toxocara spp. and hEC, Giardia spp. and C. guttulatus. Only CPV and CCoV were significantly associated with diarrhoea, hEC with a subset of puppies that had diarrhoea and severe clinical signs. CPV was more prevalent in puppies under 3 months of age. Puppies from high-volume dog breeders were significantly at increased risk for CPV (OR 4.20), CCoV (OR 4.50) and Cystoisospora spp. (OR 3.60). CCoV occurred significantly more often in winter (OR 3.35), and CPV in winter (OR 3.78) and spring (OR 4.72) as compared to summer. We conclude that routine screening for CPV, CCoV and hEC is recommended in puppies with acute diarrhoea, especially if they are under 3 months of age and originate from high-volume dog breeders. Routine screening for other pathogens may lead to less conclusive results. Copyright © 2016 The Authors. Published by Elsevier B.V. All rights reserved.

Sensitivity of outflow peaks and flood stages to the selection of dam breach parameters and simulation models

NASA Astrophysics Data System (ADS)

Singh, Krishan P.; Snorrason, Arni

1984-02-01

Important breach parameters were identified and their ranges were estimated from a detailed study of historical earthdam failures due to overtopping. The U.S. Army Corps of Engineers Hydrologic Engineering Center (HEC) and the National Weather Service (NWS) dam breach models were chosen for evaluation and simulation. Both models use similar input data and breach descriptions, but the HEC uses the hydrologic routing method (modified Puls method), whereas the NWS uses the St. Vénant equations for routing. Information on eight dams in Illinois was taken from the Corps of Engineers inspection reports, and surveyed cross-sections of the downstream channels were supplied by the Division of Water Resources of the Illinois Department of Transportation. Various combinations of breach parameters (failure time, TF; depth of overtopping, hf; and breach size, B) were used for breach simulations by both methods with the 1.00PMF, 0.50PMF and 0.25PMF (probable maximum flood) inflow hydrographs. In general, the flood stage profiles predicted by the NWS were smoother and more reasonable than those predicted by the HEC. For channels with relatively steep slopes, the methods compared fairly well, whereas for the channels with mild slope, the HEC model often predicted oscillating, erratic flood stages, mainly due to its inability to route flood waves satisfactorily in non-prismatic channels. The breach outflow peaks are affected significantly by B but less so by hf. The ratio of outflow peak to inflow peak and the effect of TF on outflow decrease as the drainage area above the dam and impounded storage increase. Flood stage profiles predicted with cross-sections taken from 7.5' maps compared favorably with those predicted using surveyed cross-sections. For the range of breach parameters studied, the range of outflow peaks and flood stages downstream from the dam can be determined for regulatory and disaster prevention measures.

Sub-hectare crop area mapped wall-to-wall in Tigray Ethiopia with HEC processing of WorldView sub-meter panchromatic image texture

NASA Astrophysics Data System (ADS)

Neigh, C. S. R.; Carroll, M.; Wooten, M.; McCarty, J. L.; Powell, B.; Husak, G. J.; Enenkel, M.; Hain, C.

2017-12-01

Global food production in the developing world occurs within sub-hectare fields that are difficult to identify with moderate resolution satellite imagery. Knowledge about the distribution of these fields is critical in food security programs. We developed a semi-automated image segmentation approach using wall-to-wall sub-meter imagery with high-end computing (HEC) to map crop area (CA) throughout Tigray, Ethiopia that encompasses over 41,000 km2. Our approach tested multiple HEC processing streams to reduce processing time and minimize mapping error. We applied multiple resolution smoothing kernels to capture differences in land surface texture associated to CA. Typically, very-small fields (mean < 2 ha) have a smooth image roughness compared to natural scrub/shrub woody vegetation at the 1 m scale and these features can be segmented in panchromatic imagery with multi-level histogram thresholding. We found multi-temporal very-high resolution (VHR) panchromatic imagery with multi-spectral VHR and moderate resolution imagery are sufficient in extracting critical CA information needed in food security programs. We produced a 2011 ‒ 2015 CA map using over 3,000 WorldView-1 panchromatic images wall-to-wall in 1/2° mosaics for Tigray, Ethiopia in 1 week. We evaluated CA estimates with nearly 3,000 WorldView-2 2 m multispectral 250 × 250 m image subsets, with seven expert interpretations, and with in-situ global positioning system (GPS) photography. Our CA estimates ranged from 32 to 41% in sub-regions of Tigray with median maximum per bin commission and omission errors of 11% and 1% respectively, with most of the error occurring in bins less than 15%. This empirical, simple, and low direct cost approach via U.S. government license agreement and HEC could be a viable big-data methodology to extract wall-to-wall CA for other regions of the world that have very-small agriculture fields with similar image texture.

Chromokinesin Kid and kinetochore kinesin CENP-E differentially support chromosome congression without end-on attachment to microtubules.

PubMed

Iemura, Kenji; Tanaka, Kozo

2015-03-06

Chromosome congression is the alignment of chromosomes at the spindle equator, and is a prerequisite for faithful chromosome segregation. Recent data suggest that before kinetochores attach to the end of microtubules (end-on attachment), chromosomes can move along microtubules towards the spindle equator through attachment of kinetochores to the lateral surface of microtubules (lateral attachment). Here we address this mechanism, focusing on the contribution of two mitotic motors, Kid and CENP-E. In cells depleted of Hec1, which is essential for end-on attachment, chromosomes show partial and transient congression. This transient congression is further perturbed by co-depletion of Kid, suggesting its role in chromosome congression. In comparison, CENP-E suppresses chromosome congression, probably by tethering kinetochores to short, unstable microtubules, and works in congression only when microtubules are stabilized. Our results may reflect the differential contributions of Kid and CENP-E in chromosome congression in physiological conditions where stabilized microtubules are becoming increased.

Effects of plane of nutrition and feed deprivation on insulin responses in dairy cattle during late gestation.

PubMed

Schoenberg, K M; Ehrhardt, R M; Overton, T R

2012-02-01

Nonlactating Holstein cows (n=12) in late pregnancy were used to determine effects of plane of nutrition followed by feed deprivation on metabolic responses to insulin. Beginning 48 d before expected parturition, cows were fed to either a high plane (HP) or a low plane (LP) of nutrition (162 and 90% of calculated energy requirements, respectively). Cows were subjected to an intravenous glucose tolerance test [GTT; 0.25 g of dextrose/kg of body weight (BW)] on d 14 of treatment and a hyperinsulinemic-euglycemic clamp (HEC; 1 μg/kg of BW/h) on d 15. Following 24 h of feed removal, cows were subjected to a second GTT on d 17 and a second HEC on d 18 after 48 h of feed removal. During the feeding period, plasma nonesterified fatty acid (NEFA) concentrations were higher for cows fed the LP diet compared with those fed the HP diet (163.6 vs. 73.1 μEq/L), whereas plasma insulin was higher for cows fed the HP diet during the feeding period (11.1 vs. 5.2 μIU/mL). Glucose areas under the curve during both GTT were higher for cows fed the LP diet than for those fed the HP diet (4,213 vs. 3,750 mg/dL × 60 min) and was higher during the GTT in the feed-deprived state (4,878 vs. 3,085 mg/dL × 60 min) than in the GTT during the fed state, suggesting slower clearance of glucose during negative energy balance either pre-or post-feed deprivation. This corresponded with a higher dextrose infusion rate during the fed-state HEC than during the feed-deprived-state HEC (203.3 vs. 90.1 mL/h). Plasma NEFA decreased at a faster rate following GTT during feed deprivation compared with that during the fed state (8.7 vs. 2.9%/min). Suppression of NEFA was highest for cows fed the HP diet during the GTT conducted during feed deprivation, and lowest for cows fed the HP diet during the fed-state GTT (68.6 vs. 50.3% decrease from basal). Plasma insulin responses to GTT were affected by feed deprivation such that cows had a much lower insulin response to GTT by 24 h after feed removal (995 vs. 3,957 μIU/mL × 60 min). During the fed-state HEC, circulating concentrations of NEFA were 21% below basal for cows fed the HP diet and 62% below basal for cows fed the LP diet; during feed deprivation, NEFA were 79 and 59% below basal for the HP and LP diets, respectively (diet × HEC). Cows that are fed below energy requirements or are feed deprived have slower clearance of glucose and greater NEFA responses to glucose challenge. Additionally, feed deprivation had a large effect on insulin secretion. Overall, effects of feed deprivation were larger than effects of plane of nutrition. Copyright © 2012 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

Remediation scenarios for attenuating peak flows and reducing sediment transport in Fountain Creek, Colorado, 2013

USGS Publications Warehouse

Kohn, Michael S.; Fulton, John W.; Williams, Cory A.; Stogner, Sr., Robert W.

2014-01-01

The U.S. Geological Survey (USGS) in cooperation with the Fountain Creek Watershed, Flood Control and Greenway District assessed remediation scenarios to attenuate peak flows and reduce sediment loads in the Fountain Creek watershed. To evaluate these strategies, the U.S. Army Corps of Engineers Hydrologic Engineering Center (HEC) hydrologic and hydraulic models were employed. The U.S. Army Corps of Engineers modeling system HEC-HMS (Hydrologic Modeling System) version 3.5 was used to simulate runoff in the Fountain Creek watershed, Colorado, associated with storms of varying magnitude and duration. Rain-gage precipitation data and radar-based precipitation data from the April 28–30, 1999, and September 14–15, 2011, storm events were used in the calibration process for the HEC-HMS model. The curve number and lag time for each subwatershed and Manning's roughness coefficients for each channel reach were adjusted within an acceptable range so that the simulated and measured streamflow hydrographs for each of the 12 USGS streamgages approximated each other. The U.S. Army Corps of Engineers modeling system HEC-RAS (River Analysis System) versions 4.1 and 4.2 were used to simulate streamflow and sediment transport, respectively, for the Fountain Creek watershed generated by a particular storm event. Data from 15 USGS streamgages were used for model calibration and 7 of those USGS streamgages were used for model validation. The calibration process consisted of comparing the simulated water-surface elevations and the cross-section-averaged velocities from the model with those surveyed in the field at the cross section at the corresponding 15 and 7 streamgages, respectively. The final Manning’s roughness coefficients were adjusted between –30 and 30 percent at the 15 calibration streamgages from the original left, right, and channel-averaged Manning's roughness coefficients upon completion of calibration. The U.S. Army Corps of Engineers modeling system HEC-RAS version 4.2 was used to simulate streamflow and sediment transport for the Fountain Creek watershed generated by a design-storm event. The Laursen-Copeland sediment-transport function was used in conjunction with the Exner 5 sorting method and the Ruby fall-velocity method to predict sediment transport. Six USGS streamgages equipped with suspended-sediment samplers were used to develop sediment-flow rating curves for the sediment-transport-model calibration. The critical Shields number in the Laursen-Copeland sediment-transport function and the volume of sediment available at a given cross section were adjusted during the HEC-RAS sediment-model calibration process. HEC-RAS model simulations used to evaluate the 14 remediation scenarios were based on unsteady-state streamflows associated with a 24-hour, 1-percent annual exceedance probability (100-year) National Oceanic and Atmospheric Administration Type II precipitation event. Scenario 0 represents the baseline or current conditions in the watershed and was used to compare the remaining 13 scenarios. Scenarios 1–8 and 12 rely on side-detention facilities to reduce peak flows and sediment transport. Scenario 9 has a diversion channel, and scenario 10 has a reservoir. Scenarios 11 and 13 incorporate channel armoring and channel widening, respectively. Scenarios 8 and 10, the scenario with the most side-detention facilities, and the scenario with the reservoir, respectively, were the most effective at reducing sediment transport and peak flow at the Pueblo, Colorado, streamgage. Scenarios 8 and 10 altered the peak flow by –58.9 and –56.4 percent, respectively. In turn, scenarios 8 and 10 altered the sediment transport by –17.7 and –62.1 percent, respectively.

Assessment of Short Term Flood Operation Strategies Using Numerical Weather Prediction Data in YUVACΙK DAM Reservoir, Turkey

NASA Astrophysics Data System (ADS)

Uysal, G.; Yavuz, O.; Sensoy, A.; Sorman, A.; Akgun, T.; Gezgin, T.

2011-12-01

Yuvacik Dam Reservoir Basin, located in the Marmara region of Turkey with 248 km2 drainage area, has steep topography, mild and rainy climate thus induces high flood potential with fast flow response, especially to early spring and fall precipitation events. Moreover, the basin provides considerable snowmelt contribution to the streamflow during melt season since the elevation ranges between 80 - 1548 m. The long term strategies are based on supplying annual demand of 142 hm3 water despite a relatively small reservoir capacity of 51 hm3. This situation makes short term release decisions as the challenging task regarding the constrained downstream safe channel capacity especially in times of floods. Providing the demand of 1.5 million populated city of Kocaeli is the highest priority issue in terms of reservoir management but risk optimization is also required due to flood regulation. Although, the spillway capacity is 1560 m3/s, the maximum amount of water to be released is set as 100 m3/s by the regional water authority taking into consideration the downstream channel capacity which passes through industrial region of the city. The reservoir is a controlled one and it is possible to hold back the 15 hm3 additional water by keeping the gates closed. Flood regulation is set to achieve the maximum possible flood attenuation by using the full flood-control zone capacity in the reservoir before making releases in excess of the downstream safe-channel capacity. However, the operators still need to exceed flood regulation zones to take precautions for drought summer periods in order to supply water without any shortage that increases the risk in times of flood. Regarding to this circumstances, a hydrological model integrated reservoir modeling system, is applied to account for the physical behavior of the system. Hence, this reservoir modeling is carried out to analyze both previous decisions and also the future scenarios as a decision support tool for operators. In the first step, a hydrological model with an embedded snow module is used to establish a rainfall-runoff relationship to calculate the inflow into the dam reservoir. The basin is divided into four sub-basins, along with the three elevation zones for each subbasin. Hydro-meteorological data are collected via 11 automated stations in and around the basin and a semi-distributed rainfall-runoff model, HEC-HMS, is calibrated for sub-basins. Then, HEC-ResSim is used to create simulation alternatives of reservoir system according to user defined guide curves and rules based on internal and/or external variables. The decision support modeling scenarios are tested with Numerical Weather Prediction Mesoscale Model 5 (MM5) daily total precipitation and daily average temperature data. Predicted precipitation and temperature data are compared with ground observations to examine the consistency. Predicted inflows computed by HEC-HMS are used as main forcing inputs into HEC-ResSim for the short term operation of reservoir during the flood events.

Search for anomalous deuterons in the reaction /sup 3/He+C. -->. d+X at p3He = 10. 8 GeV/c

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ableev, V.G.; Vorob'ev, G.G.; Gasparyan, A.P.

1985-07-01

The Alpha apparatus in a beam of 10.78-GeV/c /sup 3/He nuclei has been used to search for anomalous deuterons (demons) in the reaction /sup 3/He+C ..-->.. d+X. The deuteron yield was measured at angles theta< or approx. =20 mrad as a function of the target thickness, which varied from 0.6 to 70 cm. The data obtained exclude the production of demons in this reaction over a wide range of expected values of the cross sections for their production and interaction with matter. The technique used can be applied also to beams of relativistic nuclei in experiments to search for anomalons.

Secure Cluster Head Sensor Elections Using Signal Strength Estimation and Ordered Transmissions

PubMed Central

Wang, Gicheol; Cho, Gihwan

2009-01-01

In clustered sensor networks, electing CHs (Cluster Heads) in a secure manner is very important because they collect data from sensors and send the aggregated data to the sink. If a compromised node is elected as a CH, it can illegally acquire data from all the members and even send forged data to the sink. Nevertheless, most of the existing CH election schemes have not treated the problem of the secure CH election. Recently, random value based protocols have been proposed to resolve the secure CH election problem. However, these schemes cannot prevent an attacker from suppressing its contribution for the change of CH election result and from selectively forwarding its contribution for the disagreement of CH election result. In this paper, we propose a modified random value scheme to prevent these disturbances. Our scheme dynamically adjusts the forwarding order of contributions and discards a received contribution when its signal strength is lower than the specified level to prevent these malicious actions. The simulation results have shown that our scheme effectively prevents attackers from changing and splitting an agreement of CH election result. Also, they have shown that our scheme is relatively energy-efficient than other schemes. PMID:22408550

The use of water-soluble mucoadhesive gels for the intravesical delivery of epirubicin to the bladder for the treatment of non-muscle-invasive bladder cancer.

PubMed

Chatta, Dani; Cottrell, Lewis; Burnett, Bruce; Laverty, Garry; McConville, Christopher

2015-10-01

To develop an epirubicin-loaded, water-soluble mucoadhesive gels that have the correct rheological properties to facilitate their delivery into the bladder via a catheter, while allowing for their spread across the bladder wall with limited expansion of the bladder and increasing the retention of epirubicin in the bladder and flushing with urine. Epirubicin-loaded hydroxyl ethyl cellulose (HEC) and hydroxy propyl methyl cellulose (HPMC) gels were manufactured and tested for their rheological properties. Their ability to be pushed through a catheter was also assessed as was their in-vitro drug release, spreading in a bladder and retention of epirubicin after flushing with simulated urine. Epirubicin drug release was viscosity-dependent. The 1 and 1.5% HEC gels and the 1, 1.5 and 2% HPMC gels had the correct viscosity to be administered through a model catheter and spread evenly across the bladder wall under the pressure of the detrusor muscle. The epirubicin-loaded gels had an increased retention time in the bladder when compared with a standard intravesical solution of epirubicin, even after successive flushes with simulated urine. The increased retention of epirubicin in the bladder by the HEC and HPMC gels warrant further investigation, using an in-vivo model, to assess their potential for use as treatment for non-muscle-invasive bladder cancer. © 2015 Royal Pharmaceutical Society.

Plasma concentrations and subcutaneous adipose tissue mRNA expression of clusterin in obesity and type 2 diabetes mellitus: the effect of short-term hyperinsulinemia, very-low-calorie diet and bariatric surgery.

PubMed

Kloučková, J; Lacinová, Z; Kaválková, P; Trachta, P; Kasalický, M; Haluzíková, D; Mráz, M; Haluzík, M

2016-07-18

Clusterin is a heterodimeric glycoprotein with wide range of functions. To further explore its possible regulatory role in energy homeostasis and in adipose tissue, we measured plasma clusterin and its mRNA expression in subcutaneous adipose tissue (SCAT) of 15 healthy lean women, 15 obese women (OB) and 15 obese women with type 2 diabetes mellitus (T2DM) who underwent a 2-week very low-calorie diet (VLCD), 10 obese women without T2DM who underwent laparoscopic sleeve gastrectomy (LSG) and 8 patients with T2DM, 8 patients with impaired glucose tolerance (IGT) and 8 normoglycemic patients who underwent hyperinsulinemic euglycemic clamp (HEC). VLCD decreased plasma clusterin in OB but not in T2DM patients while LSG and HEC had no effect. Clusterin mRNA expression in SCAT at baseline was increased in OB and T2DM patients compared with controls. Clusterin mRNA expression decreased 6 months after LSG and remained decreased 12 months after LSG. mRNA expression of clusterin was elevated at the end of HEC compared with baseline only in normoglycemic but not in IGT or T2DM patients. In summary, our data suggest a possible local regulatory role for clusterin in the adipose tissue rather than its systemic involvement in the regulation of energy homeostasis.

Cosmogenic 3He age estimates of Plio-Pleistocene alluvial-fan surfaces in the Lower Colorado River Corridor, Arizona, USA

NASA Astrophysics Data System (ADS)

Fenton, Cassandra R.; Pelletier, Jon D.

2013-01-01

Plio-Pleistocene deposits of the Lower Colorado River (LCR) and tributary alluvial fans emanating from the Black Mountains near Golden Shores, Arizona record six cycles of Late Cenozoic aggradation and incision of the LCR and its adjacent alluvial fans. Cosmogenic 3He (3Hec) ages of basalt boulders on fan terraces yield age ranges of: 3.3-2.2 Ma, 2.2-1.1 Ma, 1.1 Ma to 110 ka, < 350 ka, < 150 ka, and < 63 ka. T1 and Q1 fans are especially significant, because they overlie Bullhead Alluvium, i.e. the first alluvial deposit of the LCR since its inception ca. 4.2 Ma. 3Hec data suggest that the LCR began downcutting into the Bullhead Alluvium as early as 3.3 Ma and as late as 2.2 Ma. Younger Q2a to Q4 fans very broadly correlate in number and age with alluvial terraces elsewhere in the southwestern USA. Large uncertainties in 3Hec ages preclude a temporal link between the genesis of the Black Mountain fans and specific climate transitions. Fan-terrace morphology and the absence of significant Plio-Quaternary faulting in the area, however, indicate regional, episodic increases in sediment supply, and that climate change has possibly played a role in Late Cenozoic piedmont and valley-floor aggradation in the LCR valley.

Air Emission Reduction Benefits of Biogas Electricity Generation at Municipal Wastewater Treatment Plants.

PubMed

Gingerich, Daniel B; Mauter, Meagan S

2018-02-06

Conventional processes for municipal wastewater treatment facilities are energy and materially intensive. This work quantifies the air emission implications of energy consumption, chemical use, and direct pollutant release at municipal wastewater treatment facilities across the U.S. and assesses the potential to avoid these damages by generating electricity and heat from the combustion of biogas produced during anaerobic sludge digestion. We find that embedded and on-site air emissions from municipal wastewater treatment imposed human health, environmental, and climate (HEC) damages on the order of $1.63 billion USD in 2012, with 85% of these damages attributed to the estimated consumption of 19 500 GWh of electricity by treatment processes annually, or 0.53% of the US electricity demand. An additional 11.8 million tons of biogenic CO 2 are directly emitted by wastewater treatment and sludge digestion processes currently installed at plants. Retrofitting existing wastewater treatment facilities with anaerobic sludge digestion for biogas production and biogas-fueled heat and electricity generation has the potential to reduce HEC damages by up to 24.9% relative to baseline emissions. Retrofitting only large plants (>5 MGD), where biogas generation is more likely to be economically viable, would generate HEC benefits of $254 annually. These findings reinforce the importance of accounting for use-phase embedded air emissions and spatially resolved marginal damage estimates when designing sustainable infrastructure systems.

Circulating endocannabinoids in insulin sensitive vs. insulin resistant obese postmenopausal women. A MONET group study.

PubMed

Abdulnour, Joseph; Yasari, Siham; Rabasa-Lhoret, Rémi; Faraj, May; Petrosino, Stefania; Piscitelli, Fabiana; Prud' Homme, Denis; Di Marzo, Vincenzo

2014-01-01

To measure the circulating levels of endocannabinoids and related molecules at fasting, after acute hyperinsulinemia and after weight loss in insulin sensitive vs. insulin resistant obese postmenopausal women. The sample consisted of 30 obese postmenopausal women (age: 58.9 ± 5.2 yrs; BMI: 32.9 ± 3.6 kg/m(2) ). Subjects underwent a 3-hour hyperinsulinaemic-euglycaemic clamp (HEC) (glucose disposal rate (M-value): 10.7 ± 3.3 mg min(-1) kg(-1) FFM) and 6-month weight loss intervention. Participants were classified as insulin sensitive obese (ISO) or insulin resistant obese (IRO) based on a predefined cutoff. Plasma levels of the endocannabinoids, anandamide (AEA), 2-arachidonoylglycerol (2-AG), and of the AEA-related compounds, palmitoylethanolamide (PEA) and oleoylethanolamide (OEA), were measured by liquid chromatography-mass spectrometry. IRO presented higher levels of 2-AG (P < 0.05) independently of the HEC and weight loss, whereas the HEC had an independent inhibitory effect on AEA, PEA, and OEA levels (P < 0.05) in both groups. Furthermore, there was an independent stimulatory effect of weight loss only on PEA levels in both groups (P < 0.05). This study is the first to show that higher circulating levels of the endocannabinoid 2-AG are found in IRO compared to ISO postmenopausal women, and that weight loss is associated with an increase in PEA, a PPAR-α ligand. © 2013 The Obesity Society.

Uncertainty of the peak flow reconstruction of the 1907 flood in the Ebro River in Xerta (NE Iberian Peninsula)

NASA Astrophysics Data System (ADS)

Ruiz-Bellet, Josep Lluís; Castelltort, Xavier; Balasch, J. Carles; Tuset, Jordi

2017-02-01

There is no clear, unified and accepted method to estimate the uncertainty of hydraulic modelling results. In historical floods reconstruction, due to the lower precision of input data, the magnitude of this uncertainty could reach a high value. With the objectives of giving an estimate of the peak flow error of a typical historical flood reconstruction with the model HEC-RAS and of providing a quick, simple uncertainty assessment that an end user could easily apply, the uncertainty of the reconstructed peak flow of a major flood in the Ebro River (NE Iberian Peninsula) was calculated with a set of local sensitivity analyses on six input variables. The peak flow total error was estimated at ±31% and water height was found to be the most influential variable on peak flow, followed by Manning's n. However, the latter, due to its large uncertainty, was the greatest contributor to peak flow total error. Besides, the HEC-RAS resulting peak flow was compared to the ones obtained with the 2D model Iber and with Manning's equation; all three methods gave similar peak flows. Manning's equation gave almost the same result than HEC-RAS. The main conclusion is that, to ensure the lowest peak flow error, the reliability and precision of the flood mark should be thoroughly assessed.

Evaluation of the lime-cooking and tortilla making properties of quality protein maize hybrids grown in Mexico.

PubMed

Serna-Saldivar, Sergio O; Amaya Guerra, Carlos A; Herrera Macias, Pedro; Melesio Cuellar, Jose L; Preciado Ortiz, Ricardo E; Terron Ibarra, Arturo D; Vazquez Carrillo, Gricelda

2008-09-01

Eleven experimental and three commercial white quality protein maize (QPM) hybrids and two regular endosperm controls were planted at Celaya, Guanajuato, Mexico with the aim of comparing grain physical characteristics, protein quality, lime-cooking and tortilla making properties. All genotypes were planted under irrigation using a density of 80,000 plants/ha and fertilized with 250 kg N-60 P-60 K per hectare. When compared with the controls these QPM genotypes had lower test (77.4 vs. 76.5 kg/hL) and 1,000 kernel weights (327 vs. 307 g), softer endosperm texture (2.5 vs. 1.8 where 1 = soft, 2 intermediate and 3 hard endosperm), lower protein (10.0 vs. 8.0%), higher nixtamal water uptake after 30 min lime-cooking (50.0 vs. 53.1% moisture) and lower pericarp removal scores. The lower thousand-kernel weight and softer endosperm texture observed in the QPM genotypes lowered the optimum lime-cooking time as estimated with regression equations. Most QPM genotypes had higher amounts of lysine, tryptophan and albumins/globulins when compared with the controls. QPMs HEC 424973, HEC 774986 and HEC 734286 had the best grain traits for nixtamalization and therefore the best potential for industrial utilization. The commercial use of these QPM hybrids should benefit Mexicans who depend on tortillas as the main staple.

Screening of plant extracts for anthelmintic activity against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus).

PubMed

Huang, Ai-Guo; Yi, Yang-Lei; Ling, Fei; Lu, Lin; Zhang, Qi-Zhong; Wang, Gao-Xue

2013-12-01

With the aim of finding natural anthelmintic agents against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus), 26 plants were screened for antiparasitic properties using in vivo anthelmintic efficacy assay. The results showed that Caesalpinia sappan, Lysima chiachristinae, Cuscuta chinensis, Artemisia argyi, and Eupatorium fortunei were found to have 100% anthelmintic efficacy at 125, 150, 225, 300, and 500 mg L(-1) after 48 h of exposure. Crude extract of the five plants were further partitioned with petroleum ether, chloroform, ethyl acetate, methanol, and water to obtain anthelmintically active fractions with various polarity. Among these fractions tested, the ethyl acetate extract of L. chiachristinae was found to be the most effective with a 50% effective concentration (EC50) value of 5.1 mg/L after 48 h of exposure. This was followed by ethyl acetate extract of C. chinensis (48 h-EC50 = 8.5 mg L(-1)), chloroform extracts of C. sappan (48 h-EC50 = 15.6 mg L(-1)), methanol extract of C. chinensis (48 h-EC50 = 15.9 mg L(-1)), and chloroform and petroleum ether extract of L. chiachristinae (EC50 values of 17.2 and 21.1 mg/L, respectively), suggesting that these plants, as well as the active fractions, provide potential sources of botanic drugs for the control of D. intermedius in aquaculture.

EPA's National Coastal Condition Assessment: Pilot research ...

EPA Pesticide Factsheets

The EPA Office of Water’s 5-year cycles of national surveys of wetlands, lakes, rivers, and coastal areas help satisfy the assessment and antidegradation provisions of the Clean Water Act. Measuring extant conditions precedes measuring change in conditions. Surveys are challenged to adequately sample extreme conditions occurring in small areas. Extremely bad conditions are targets for remediation. Extremely good conditions are targets for protection. In 2010, the National Coastal Condition Assessment (NCCA) found the majority of the coastal Great Lakes (by area) was in good condition for water (60%) and sediment (51%) quality but not benthos (20%) and fish tissue contaminants (<1%). Low sampling success for biological sampling was an issue. As part of the 2014 Lake Erie CSMI field year, EPA’s Great Lakes National Program Office, working with the Office of Research and Development, began pilot research to integrate connecting channels into Great Lakes surveys. Assessments of the Huron-Erie corridor (HEC; 2014, 2015) and St Marys River (SMR; 2015, 2016) which have previously gone unassessed by NCCA, are being developed. Water, sediment, and benthic quality data from the 2014 HEC survey (n=60) were compared to 2010 NCCA data from adjacent lakes. Water quality rated “poor” (as % area) in HEC was intermediate compared to Lake Huron and Erie regardless of which lake-specific thresholds were used. However, the amount of area classified as “good” was highl

Human Antibodies to PhtD, PcpA, and Ply Reduce Adherence to Human Lung Epithelial Cells and Murine Nasopharyngeal Colonization by Streptococcus pneumoniae

PubMed Central

Kaur, Ravinder; Surendran, Naveen; Ochs, Martina

2014-01-01

Streptococcus pneumoniae adherence to human epithelial cells (HECs) is the first step in pathogenesis leading to infections. We sought to determine the role of human antibodies against S. pneumoniae protein vaccine candidates PhtD, PcpA, and Ply in preventing adherence to lung HECs in vitro and mouse nasopharyngeal (NP) colonization in vivo. Human anti-PhtD, -PcpA, and -Ply antibodies were purified and Fab fragments generated. Fabs were used to test inhibition of adherence of TIGR4 and nonencapsulated strain RX1 to A549 lung HECs. The roles of individual proteins in adherence were tested using isogenic mutants of strain TIGR4. Anti-PhtD, -PcpA, and -Ply human antibodies were assessed for their ability to inhibit NP colonization in vivo by passive transfer of human antibody in a murine model. Human antibodies generated against PhtD and PcpA caused a decrease in adherence to A549 cells (P < 0.05). Anti-PhtD but not anti-PcpA antibodies showed a protective role against mouse NP colonization. To our surprise, anti-Ply antibodies also caused a significant (P < 0.05) reduction in S. pneumoniae colonization. Our results support the potential of PhtD, PcpA, and Ply protein vaccine candidates as alternatives to conjugate vaccines to prevent non-serotype-specific S. pneumoniae colonization and invasive infection. PMID:25245804

[Transforming gene in human esophageal carcinoma tissue].

PubMed

Jiang, W

1988-09-01

The transforming gene in human esophageal carcinoma (HEC) tissues collected from Lin-xian county, a high incidence area of esophageal cancer was studied. Eight primary HEC tissues were used as sources for the preparation of DNA. High molecular weight DNAs were separately added to NIH 3T3 cells by the calcium phosphate coprecipitation method. Of the 8 HEC tissues examined, 3 DNAs showed transforming activity and produced secondary transformants. The use of uncloned NIH 3T3 cells resulted in the appearances of non-transforming. The efficiency of primary transfection foci was low (0.025--0.05 focus per ug of DNA). In the secondary transfection, the efficiency was increased (0.30 focus per ug of DNA). The primary and secondary transformants were capable of forming colonies in soft agar (0.33%) in contrast to the control NIH 3T3 cells, which did not show any anchorage-independent growth. About 1 X 10(6) cells of the cloned secondary transformants were injected subcutaneously into athymic BALB/c nude mice. The mice developed large tumors (approximately 20-30 mm in diameter) within 5--15 days after injection. No tumor developed in mice injected with control NIH 3T3 cells even after 2 months. The transforming DNA had a linkage to the Alu sequence, indicating that a common human DNA fragment is conserved in the tumors. H-ras was found in the transforming DNA using Southern blot assay.

The further environmental development of Polyphyto Hydroelectric Project reservoir in Kozani prefecture and its contribution to the life quality improvement

NASA Astrophysics Data System (ADS)

Saounatsou, Chara; Georgi, Julia

2014-08-01

The Polyphyto Hydroelectric Project was constructed in 1974 and it has been operating since on the Aliakmonas River, Kozani prefecture, by the Greek Public Power Corporation. The construction of the Ilarion Hydroelectric Project, upstream from the Polyphyto Reservoir, has been recently completed and will start operating in the near future. Apart from hydroelectric power production, the Polyphyto reservoir provides flood control to the areas below the Polyphyto dam. It is also used to manage water provision to the city of Thessaloniki and adjacent agricultural plain, providing at the same time cooling water to the Thermo Electric Projects in Ptolemaida. The Polyphyto reservoir has potential for further development as an economic fulcrum to the region in which is located. The Kozani and Servia-Velvendos Municipalities have proceeded to the construction of several touristic, nautical - athletic and fishing projects. In order to promote such developments, while preserving the artificial wetland, flora and fauna of the Polyphyto Reservoir, it is important to reduce the fluctuation of the reservoir elevation which according to its technical characteristics is 21m. The aim of this paper is to propose the combined operation of the two Hydroelectric Project reservoirs to satisfy all the present Polyphyto Hydroelectric Project functions and to reduce the annual fluctuation of the Polyphyto Reservoir. The HEC-5, Version 8 / 1998 computer model was used in our calculations, as developed by the Hydrologic Engineering Center (HEC) of the US Army Corps of Engineers for reservoir operation simulation. Five possible operation scenarios are tested in this paper to show that the present fluctuation of the Polyphyto Reservoir can be reduced, with some limitations, except during dry weather periods.

Prototypes of self-powered radiation detectors employing intrinsic high-energy current.

PubMed

Zygmanski, Piotr; Shrestha, Suman; Briovio, Davide; Karellas, Andrew; Sajo, Erno

2016-01-01

The authors experimentally investigate the effect of direct energy conversion of x-rays via selfpowered Auger- and photocurrent, potentially suitable to practical radiation detection and dosimetry in medical applications. Experimental results are compared to computational predictions. The detector the authors consider is a thin-film multilayer device, composed of alternating disparate electrically conductive and insulating layers. This paper focuses on the experiments while a companion paper introduces the fundamental concepts of high-energy current (HEC) detectors. The energy of ionizing radiation is directly converted to detector signal via electric current induced by high-energy secondary electrons generated in the detector material by the incident primary radiation. The HEC electrons also ionize the dielectric and the resultant charge carriers are selfcollected due to the contact potential of the disparate electrodes. Thus, an electric current is induced in the conductors in two different ways without the need for externally applied bias voltage or amplification. Thus, generated signal in turn is digitized by a data acquisition system. To determine the fundamental properties of the HEC detector and to demonstrate its feasibility for medical applications, the authors used a planar geometry composed of multilayer microstructures. Various detectors with up to seven conducting layers with different combinations of materials (250 μm Al, 35 μm Cu, 100 μm Pb) and air gaps (100 μm) were exposed to nearly plane-parallel 60-120 kVp x-ray beams. For the experimental design and verification, the authors performed coupled electron-photon radiation transport computations. The detector signal was measured using a commercial data acquisition system with 24 bits dynamic range, 0.4 fC sensitivity, and 0.9 ms sampling time. Measured signals for the prototype detector varied depending on the number of layers, material type, and incident photon energy, and it was in the range of 30-150 nA/cm(2) for unit air kerma (1 Gy), which is viable for practical applications. The experiments had an excellent agreement with the computations. Within the examined range of 60-120 kVp, the energy dependence of the HEC (normalized to the x-ray tube output) was relatively small. Based on the experimental results for 100 ms sampling time, it would be possible to measure the time dependence of x-ray beams for x-ray tube current of 0.1 mA or higher. Significant advantages of the HEC device are that generation of its signal does not require external power supply, it can be made in any size and shape, including flexible curvilinear forms, and it is inexpensive. It remains to be determined, which of the potential applications in medical dosimetry (both in vivo and external), or radiation protection would benefit from such selfpowered detectors.

[Influence of pertussis toxin on GPER-mediated activation of phosphatidylinositol 3-kinase/protein kinase B signaling induced by 17β-estradiol in endometrial carcinoma cells].

PubMed

Guo, Rui-xia; Lei, Jia; Wang, Xin-yan; Ge, Xin; Hu, Dong-mei; Ma, Xiu-ying; Li, Liu-xia; Qiao, Yu-huan

2013-02-01

To investigate the influence of pertussis toxin (PTX) on G protein-coupled estrogen receptor (GPER)-mediated activation of phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signaling activated by 17β-estradiol (17β-E2) in endometrial carcinoma cells. Expressions of GPER protein were detected by immunohistochemical SP method in Ishikawa and HEC-1A cells. Changes of levels of GPER, ERα and ERβ protein and the activation of Akt protein were observed by western blot in the two cells after they were treated by PTX for 30 minutes at different concentrations (0, 0.1, 0.5, 1.0 µg/ml), and then co-stimulated with with 1×10(-6) mol/L 17β-E2 respectively at different time (Ishikawa 30 minutes, HEC-1A 15 minutes). (1) Immunohistochemical SP method showed that GPER was positive stained in cell cytoplasm of Ishikawa and HEC-1A cell. (2) After co-treated with PTX at different concentrations (0, 0.1, 0.5, 1.0 µg/ml) and 10(-6) mol/L 17β-E2, in Ishikawa cell, the ratio of p-Akt/Akt was 0.74 ± 0.54, 0.34 ± 0.06, 0.18 ± 0.03, 0.07 ± 0.15, the gray values of GPER was 0.872 ± 0.490, 0.395 ± 0.054, 0.145 ± 0.014, 0.034 ± 0.008, and with increasing concentration of PTX, the ratio of p-Akt/Akt and the expression of GPER decreased gradually (P < 0.05), which was most obviously when the concentration was 1.0 µg/ml (F = 63.729, P = 0.0001; F = 160.284, P = 0.0001); ERα and ERβ protein had no significant change among different groups (P > 0.05). In HEC-1A cell, the ratio of p-Akt/Akt was 0.73 ± 0.09, 0.26 ± 0.14, 0.11 ± 0.03, 0, the Gray values of GPER is 0.927 ± 0.134, 0.485 ± 0.022, 0.194 ± 0.004, 0, and with increasing concentration of PTX, the ratio of p-Akt/Akt and the expression of GPER decreased gradually (P < 0.05), which were also completely inhibited when the concentration was 1 µg/ml (F = 1039.321, P = 0.0001; F = 109.646, P = 0.0001), ERα protein had no significant differences (P > 0.05) among different groups. ERβ was negatively expressed. The results proposed that the activation of PI3K/Akt signaling in Ishikawa and HEC-1A cells could be inhibited after blocking the role of GPER by PTX.

Massive star-forming regions across the galaxy

NASA Astrophysics Data System (ADS)

Rygl, Kazi Lucie Jessica

2010-04-01

Star-forming regions trace the spiral structure of the Galaxy. They are regions of increased column density and therefore traced well by the extinction in the mid-infrared based on the Spitzer/GLIMPSE 3.6-4.5 micron color excess maps. A sample of 25 high extinction clouds (HECs) was studied in the 1.2 mm dust continuum emission, and followed up by observations of ammonia plus several other molecules using the Effelsberg 100m, IRAM 30m and APEX telescopes. With these data we want to investigate the most early stages of massive star formation, which are currently still largely unknown. Three cloud classes were defined from their morphology in the 1.2 mm continuum maps: the early diffuse HECs, with a low contrast between the clump and cloud emission; the peaked HECs, with an increased contrast; the late multiply peaked HECs, with more than one clump and a high contrast between the clump and the cloud emission. The clouds are cold (T 16 K) and massive (M 800 M_sun) and contain dense clumps (n 10^5 cm^{-3}) of 0.3 pc in size. These clumps were investigated for evidence of gravitational collapse or expansion, for high velocity outflows, and for the presence of young stellar objects. Based on these results we interpret the three cloud classes as an evolutionary sequence of star-forming clouds. Accurate distances are a crucial parameter for establishing the mass, size, and luminosity of an object. Also, for understanding the spiral structure of the Galaxy trustworthy distances are necessary. The most accurate method to measure these is the trigonometric parallax. Using the European Very Large Baseline Interferometry Network of radio antennas we measured, for the first time, parallaxes of 6.7 GHz methanol masers. This transition belongs to the strongest maser species in the Galaxy, it is stable and observed toward numerous massive star-forming regions. We measured distances and proper motions toward L 1287, L 1206, NGC 281-W, ON 1 and S 255, and obtained their 3-dimensional space velocities. Similar to previous studies, these star-forming regions rotate slower than Galactic rotation.

Combined Hydrologic (AGWA-KINEROS2) and Hydraulic (HEC2) Modeling for Post-Fire Runoff and Inundation Risk Assessment through a Set of Python Tools

NASA Astrophysics Data System (ADS)

Barlow, J. E.; Goodrich, D. C.; Guertin, D. P.; Burns, I. S.

2016-12-01

Wildfires in the Western United States can alter landscapes by removing vegetation and changing soil properties. These altered landscapes produce more runoff than pre-fire landscapes which can lead to post-fire flooding that can damage infrastructure and impair natural resources. Resources, structures, historical artifacts and others that could be impacted by increased runoff are considered values at risk. .The Automated Geospatial Watershed Assessment tool (AGWA) allows users to quickly set up and execute the Kinematic Runoff and Erosion model (KINEROS2 or K2) in the ESRI ArcMap environment. The AGWA-K2 workflow leverages the visualization capabilities of GIS to facilitate evaluation of rapid watershed assessments for post-fire planning efforts. High relative change in peak discharge, as simulated by K2, provides a visual and numeric indicator to investigate those channels in the watershed that should be evaluated for more detailed analysis, especially if values at risk are within or near that channel. Modeling inundation extent along a channel would provide more specific guidance about risk along a channel. HEC-2 and HEC-RAS can be used for hydraulic modeling efforts at the reach and river system scale. These models have been used to address flood boundaries and, accordingly, flood risk. However, data collection and organization for hydraulic models can be time consuming and therefore a combined hydrologic-hydraulic modeling approach is not often employed for rapid assessments. A simplified approach could streamline this process and provide managers with a simple workflow and tool to perform a quick risk assessment for a single reach. By focusing on a single reach highlighted by large relative change in peak discharge, data collection efforts can be minimized and the hydraulic computations can be performed to supplement risk analysis. The incorporation of hydraulic analysis through a suite of Python tools (as outlined by HEC-2) with AGWA-K2 will allow more rapid applications of combined hydrologic-hydraulic modeling. This combined modeling approach is built in the ESRI ArcGIS application to enable rapid model preparation, execution and result visualization for risk assessment in post-fire environments.

Antitumor effect of the selective COX-2 inhibitor celecoxib on endometrial adenocarcinoma in vitro and in vivo

PubMed Central

XIAO, YITAO; TENG, YINCHENG; ZHANG, RUI; LUO, LAIMIN

2012-01-01

The aim of this study was to investigate the antitumor effect of the selective cyclooxygenase-2 (COX-2) inhibitor celecoxib on endometrial adenocarcinoma in mice. Various amounts of celecoxib were added to HEC-1B cells in vitro for different durations. Cell cycle and apoptosis were analyzed using flow cytometry. HEC-1B cytostasis, invasiveness and COX-2 expression were examined by MTT, transwell cabin and western blot assays, respectively. An in vivo human endometrial adenocarcinoma model was established in BALB/c nude mice using HEC-1B cells. For two weeks, the celecoxib groups were treated with celecoxib 2 or 4 mg/day via oral administration and the control group was treated with saline. Tumor volume, growth curves and the inhibition rate (IR) were recorded. COX-2 expression levels and microvessel density (MVD) were investigated using an immunohistochemical technique. In the celecoxib groups, cell proliferation was significantly inhibited in a concentration- and time-dependent manner. The proportion of cells in the G0/G1 phase increased within 24 h after the addition of celecoxib whereas those in the S and G2/M phases decreased with an increasing apoptosis peak (sub-G1) and apoptosis rate. The microporous Matrigel-coated polycarbonate membrane of the Transwell cabin was traversable for the HEC-1B cells. The invasiveness was attenuated when the celecoxib concentration was increased. The tumor growth was also greatly inhibited when the celecoxib concentration was increased. The tumor IRs were 32.4 and 48.6% following treatment with 2 and 4 mg/day celecoxib, respectively. COX-2 was mainly expressed in the cytoplasm of the tumor cells. In the celecoxib groups, the COX-2 expression levels were concentration-dependent. The COX-2 expression level and MVD decreased when the celecoxib concentration was increased. The results of dependability analysis revealed that the COX-2 expression level was positively correlated with MVD (r=0.921; P<0.01). The antitumor effect of celecoxib on endometrial adenocarcinoma in nude mice may be related to the inhibition of COX-2 expression and microangiogenesis. PMID:23226798

Integrating Satellite Image Identification and River Routing Simulation into the Groundwater Simulation of Chou-Shui Basin

NASA Astrophysics Data System (ADS)

Yao, Y.; Yang, S.; Chen, Y.; Chang, L.; Chiang, C.; Huang, C.; Chen, J.

2012-12-01

Many groundwater simulation models have been developed for Chou-Shui River alluvial fan which is one of the most important groundwater areas in Taiwan. However, the exchange quantity between Chou-Shui River, the major river in this area, and the groundwater system itself is seldom studied. In this study, the exchange is evaluated using a river package (RIV) in the groundwater simulation model, MODFLOW 2000. Several critical parameters and variables used in RIV such as wet area and river level for each cell below the Chou-Shui River are respectively determined by satellite image identification and HEC-RAS simulation. The monthly average of river levels obtained from four stations include Chang-Yun Bridge, Xi-Bin Bridge, Chi-Chiang Bridge and Si-Jou Bridge during 2008 and the river cross-section measured on December 2007 are used in the construction of HEC-RAS model. Four FORMOSAT multispectral satellite images respectively obtained on January 2008, April 2008, July 2008, and November 2008 are used to identify the wet area of Chou-Shui River during different seasons. Integrating the simulation level provided by HEC-RAS and the identification result are used as the assignment of RIV. First, based on the simulation results of HEC-RAS, the water level differences between flooding period and draught period are 1.4 (m) and 2.0 (m) for Xi-Bin Bridge station (downstream) and Chang-Yun Bridge station (upstream) respectively. Second, based on the identified results, the wet areas for four seasons are 24, 24, 40 and 12 (km2) respectively. The variation range of areas in 2008 is huge that the area for winter is just 30% of the area for summer. Third, based on the simulation of MODFLOW 2000 and RIV, the exchange between the river and the groundwater system is 414 million cubic meters which contains 526 for recharge to river and 112 for discharging from river during 2008. The total recharge includes river exchange and recharge from non-river area is 2023 million cubic meters. The pumping quantity is 1930 million cubic meters.

NEPA, a new fixed combination of netupitant and palonosetron, is a cost-effective intervention for the prevention of chemotherapy-induced nausea and vomiting in the UK

PubMed Central

Cawston, Helene; Bourhis, Francois; Eriksson, Jennifer; Ruffo, Pierfrancesco; D’Agostino, Paolo; Turini, Marco; Schwartzberg, Lee; McGuire, Alistair

2017-01-01

Background The objective was to evaluate the cost-effectiveness of NEPA, an oral fixed combination netupitant (NETU, 300 mg) and palonosetron (PA, 0.5 mg) compared with aprepitant and palonosetron (APPA) or palonosetron (PA) alone, to prevent chemotherapy-induced nausea and vomiting (CINV) in patients undergoing treatment with highly or moderately emetogenic chemotherapy (HEC or MEC) in the UK. Scope A systematic literature review and meta-analysis were undertaken to compare NEPA with currently recommended anti-emetics. Relative effectiveness was estimated over the acute (day 1) and overall treatment (days 1–5) phases, taking complete response (CR, no emesis and no rescue medication) and complete protection (CP, CR and no more than mild nausea [VAS scale <25 mm]) as primary efficacy outcomes. A three-health-state Markov cohort model, including CP, CR and incomplete response (no CR) for HEC and MEC, was constructed. A five-day time horizon and UK NHS perspective were adopted. Transition probabilities were obtained by combining the response rates of CR and CP from NEPA trials and odds ratios from the meta-analysis. Utilities of 0.90, 0.70 and 0.24 were defined for CP, CR and incomplete response, respectively. Costs included medications and management of CINV-related events and were obtained from the British National Formulary and NHS Reference Costs. The expected budgetary impact of NEPA was also evaluated. Findings In HEC patients, the NEPA strategy was more effective than APPA (quality-adjusted life days [QALDs] of 4.263 versus 4.053; incremental emesis-free and CINV-free days of +0.354 and +0.237, respectively) and was less costly (£80 versus £124), resulting in NEPA being the dominant strategy. In MEC patients, NEPA was cost effective, cumulating in an estimated 0.182 extra QALDs at an incremental cost of £6.65 compared with PA. Conclusion Despite study limitations (study setting, time horizon, utility measure), the results suggest NEPA is cost effective for preventing CINV associated with HEC and MEC in the UK. PMID:28392826

A Reliability Estimation in Modeling Watershed Runoff With Uncertainties

NASA Astrophysics Data System (ADS)

Melching, Charles S.; Yen, Ben Chie; Wenzel, Harry G., Jr.

1990-10-01

The reliability of simulation results produced by watershed runoff models is a function of uncertainties in nature, data, model parameters, and model structure. A framework is presented here for using a reliability analysis method (such as first-order second-moment techniques or Monte Carlo simulation) to evaluate the combined effect of the uncertainties on the reliability of output hydrographs from hydrologic models. For a given event the prediction reliability can be expressed in terms of the probability distribution of the estimated hydrologic variable. The peak discharge probability for a watershed in Illinois using the HEC-1 watershed model is given as an example. The study of the reliability of predictions from watershed models provides useful information on the stochastic nature of output from deterministic models subject to uncertainties and identifies the relative contribution of the various uncertainties to unreliability of model predictions.

Design of bridge deck drainage : HEC 21

DOT National Transportation Integrated Search

1993-05-01

The manual provides guidelines and procedures for designing bridge deck drainage systems, inclusing illustrative examples. Should the design process indicate a drainage system is needed, utilization of the most hydraulically efficient and maintenance...

Transbuccal delivery of chlorpheniramine maleate from mucoadhesive buccal patches.

PubMed

Sekhar, K Chandra; Naidu, K V S; Vishnu, Y Vamshi; Gannu, Ramesh; Kishan, V; Rao, Y Madhusudan

2008-01-01

This article describes buccal permeation of chlorpheniramine maleate (CPM) and its transbuccal delivery using mucoadhesive buccal patches. Permeation of CPM was calculated in vitro using porcine buccal membrane and in vivo in healthy humans. Buccal formulations were developed with hydroxyethylcellulose (HEC) and evaluated for in vitro release, moisture absorption, mechanical properties, and bioadhesion, and optimized formulation was subjected for bioavailability studies in healthy human volunteers. In vitro flux of CPM was calculated to be 0.14 +/- 0.03 mg.h(-1).cm(-2) and buccal absorption also was demonstrated in vivo in human volunteers. In vitro drug release and moisture absorbed were governed by HEC content and formulations exhibited good tensile and mucoadhesive properties. Bioavailability from optimized buccal patch was 1.46 times higher than the oral dosage form and the results showed statistically significant difference.

Comparative Study of Antimicrobial Activity of AgBr and Ag Nanoparticles (NPs)

PubMed Central

Suchomel, Petr; Kvitek, Libor; Panacek, Ales; Prucek, Robert; Hrbac, Jan; Vecerova, Renata; Zboril, Radek

2015-01-01

The diverse mechanism of antimicrobial activity of Ag and AgBr nanoparticles against gram-positive and gram-negative bacteria and also against several strains of candida was explored in this study. The AgBr nanoparticles (NPs) were prepared by simple precipitation of silver nitrate by potassium bromide in the presence of stabilizing polymers. The used polymers (PEG, PVP, PVA, and HEC) influence significantly the size of the prepared AgBr NPs dependently on the mode of interaction of polymer with Ag+ ions. Small NPs (diameter of about 60–70 nm) were formed in the presence of the polymer with low interaction as are PEG and HEC, the polymers which interact with Ag+ strongly produce nearly two times bigger NPs (120–130 nm). The prepared AgBr NPs were transformed to Ag NPs by the reduction using NaBH4. The sizes of the produced Ag NPs followed the same trends – the smallest NPs were produced in the presence of PEG and HEC polymers. Prepared AgBr and Ag NPs dispersions were tested for their biological activity. The obtained results of antimicrobial activity of AgBr and Ag NPs are discussed in terms of possible mechanism of the action of these NPs against tested microbial strains. The AgBr NPs are more effective against gram-negative bacteria and tested yeast strains while Ag NPs show the best antibacterial action against gram-positive bacteria strains. PMID:25781988

Physicochemical properties of pH-sensitive hydrogels based on hydroxyethyl cellulose-hyaluronic acid and for applications as transdermal delivery systems for skin lesions.

PubMed

Kwon, Soon Sik; Kong, Bong Ju; Park, Soo Nam

2015-05-01

We investigated the physicochemical properties of pH-sensitive hydroxyethyl cellulose (HEC)/hyaluronic acid (HA) complex hydrogels containing isoliquiritigenin (ILTG), and discussed potential applications as transdermal delivery systems for the treatment of skin lesions caused by pH imbalance. HA has skin compatibility and pH functional groups and HEC serves as scaffold to build hydrogels with varied HCE:HA mass ratio. Hydrogels were synthesized via chemical cross-linking, and three-dimensional network structures were characterized via scanning electron microscopy (SEM). The swelling properties and polymer ratios of the hydrogels were investigated at pH values in the range 1-13. HECHA13 (i.e., an HEC:HA mass ratio of 1:3) was found to have optimal rheological and adhesive properties, and was used to investigate the drug release efficiency as a function of pH; the efficiency was greater than 70% at pH 7. Antimicrobial activity assays against Propionibacterium acnes were conducted to take advantage of the pH-sensitive properties of HECHA13. At pH 7, we found that HECHA13, which contained ILTG, inhibited the growth of P. acnes. Furthermore, HECHA13 was found to exhibit excellent permeability into the skin, which penetrated mostly via the hair follicle. These results indicate that this pH-sensitive hydrogel is effective as a transdermal delivery system for antimicrobial therapeutics, with potential applications in the treatment of acne. Copyright © 2015 Elsevier B.V. All rights reserved.

A bipartite signal mediates the transfer of type IV secretion substrates of Bartonella henselae into human cells.

PubMed

Schulein, Ralf; Guye, Patrick; Rhomberg, Thomas A; Schmid, Michael C; Schröder, Gunnar; Vergunst, Annette C; Carena, Ilaria; Dehio, Christoph

2005-01-18

Bacterial type IV secretion (T4S) systems mediate the transfer of macromolecular substrates into various target cells, e.g., the conjugative transfer of DNA into bacteria or the transfer of virulence proteins into eukaryotic host cells. The T4S apparatus VirB of the vascular tumor-inducing pathogen Bartonella henselae causes subversion of human endothelial cell (HEC) function. Here we report the identification of multiple protein substrates of VirB, which, upon translocation into HEC, mediate all known VirB-dependent cellular changes. These Bartonella-translocated effector proteins (Beps) A-G are encoded together with the VirB system and the T4S coupling protein VirD4 on a Bartonella-specific pathogenicity island. The Beps display a modular architecture, suggesting an evolution by extensive domain duplication and reshuffling. The C terminus of each Bep harbors at least one copy of the Bep-intracellular delivery domain and a short positively charged tail sequence. This biparte C terminus constitutes a transfer signal that is sufficient to mediate VirB/VirD4-dependent intracellular delivery of reporter protein fusions. The Bep-intracellular delivery domain is also present in conjugative relaxases of bacterial conjugation systems. We exemplarily show that the C terminus of such a conjugative relaxase mediates protein transfer through the Bartonella henselae VirB/VirD4 system into HEC. Conjugative relaxases may thus represent the evolutionary origin of the here defined T4S signal for protein transfer into human cells.

Human antibodies to PhtD, PcpA, and Ply reduce adherence to human lung epithelial cells and murine nasopharyngeal colonization by Streptococcus pneumoniae.

PubMed

Kaur, Ravinder; Surendran, Naveen; Ochs, Martina; Pichichero, Michael E

2014-12-01

Streptococcus pneumoniae adherence to human epithelial cells (HECs) is the first step in pathogenesis leading to infections. We sought to determine the role of human antibodies against S. pneumoniae protein vaccine candidates PhtD, PcpA, and Ply in preventing adherence to lung HECs in vitro and mouse nasopharyngeal (NP) colonization in vivo. Human anti-PhtD, -PcpA, and -Ply antibodies were purified and Fab fragments generated. Fabs were used to test inhibition of adherence of TIGR4 and nonencapsulated strain RX1 to A549 lung HECs. The roles of individual proteins in adherence were tested using isogenic mutants of strain TIGR4. Anti-PhtD, -PcpA, and -Ply human antibodies were assessed for their ability to inhibit NP colonization in vivo by passive transfer of human antibody in a murine model. Human antibodies generated against PhtD and PcpA caused a decrease in adherence to A549 cells (P < 0.05). Anti-PhtD but not anti-PcpA antibodies showed a protective role against mouse NP colonization. To our surprise, anti-Ply antibodies also caused a significant (P < 0.05) reduction in S. pneumoniae colonization. Our results support the potential of PhtD, PcpA, and Ply protein vaccine candidates as alternatives to conjugate vaccines to prevent non-serotype-specific S. pneumoniae colonization and invasive infection. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

A description of the nearshore fish communities in the Huron-Erie Corridor using multiple gear types

USGS Publications Warehouse

Francis, James T.; Chiotti, Justin A.; Boase, James C.; Thomas, Mike V.; Manny, Bruce A.; Roseman, Edward F.

2013-01-01

Great Lakes coastal wetlands provide a critical habitat for many fish species throughout their life cycles. Once home to one of the largest wetland complexes in the Great Lakes, coastal wetlands in the Huron–Erie Corridor (HEC) have decreased dramatically since the early 1900s. We characterized the nearshore fish communities at three different wetland complexes in the HEC using electrofishing, seines, and fyke nets. Species richness was highest in the Detroit River (63), followed by the St. Clair Delta (56), and Western Lake Erie (47). The nearshore fish communities in the Detroit River and St. Clair Delta consisted primarily of shiners, bluntnose minnow, centrarchids, and brook silverside, while the Western Lake Erie sites consisted of high proportions of non-native taxa including common carp, gizzard shad, goldfish, and white perch. Species richness estimates using individual-based rarefaction curves were higher when using electrofishing data compared to fyke nets or seine hauls at each wetland. Twelve fish species were captured exclusively during electrofishing assessments, while one species was captured exclusively in fyke nets, and none exclusively during seine hauls. Western Lake Erie wetlands were more indicative of degraded systems with lower species richness, lower proportion of turbidity intolerant species, and increased abundance of non-native taxa. This work highlights the importance of coastal wetlands in the HEC by capturing 69 different fish species utilizing these wetlands to fulfill life history requirements and provides insight when selecting gears to sample nearshore littoral areas.

More intensive CMV-infection in chronic heart failure patients contributes to higher T-lymphocyte differentiation degree.

PubMed

Moro-García, Marco Antonio; López-Iglesias, Fernando; Marcos-Fernández, Raquel; Bueno-García, Eva; Díaz-Molina, Beatriz; Lambert, José Luis; Suárez-García, Francisco Manuel; Morís de la Tassa, Cesar; Alonso-Arias, Rebeca

2018-03-30

Immunosenescence in chronic heart failure (CHF) is characterized by a high frequency of differentiated T-lymphocytes, contributing to an inflammatory status and a deficient ability to generate immunocompetent responses. CMV is the best known inducer of T-lymphocyte differentiation, and is associated with the phenomenon of immunosenescence. In this study, we included 58 elderly chronic heart failure patients (ECHF), 60 healthy elderly controls (HEC), 40 young chronic heart failure patients (YCHF) and 40 healthy young controls (HYC). High differentiation of CD8+ T-lymphocytes was found in CMV-seropositive patients; however, the differentiation of CD4+ T-lymphocytes was increased in CMV-seropositive but also in CHF patients. Anti-CMV antibody titers showed positive correlation with more differentiated CD4+ and CD8+ subsets and inverse correlation with CD4/CD8 ratio. Immunosenescence found in CHF patients is mainly due to the dynamics of CMV-infection, since the differentiation of T-lymphocyte subsets is related not only to CMV-infection, but also to anti-CMV antibody titers. Copyright © 2018 Elsevier Inc. All rights reserved.

Work-Centered Technology Development (WTD)

DTIC Science & Technology

2005-03-01

theoretical, testable, inductive, and repeatable foundations of science. o Theoretical foundations include notions such as statistical versus analytical...Human Factors and Ergonomics Society, 263-267. 179 Eggleston, R. G. (2005). Coursebook : Work-Centered Design (WCD). AFRL/HECS WCD course training

NASA Advanced Supercomputing Facility Expansion

NASA Technical Reports Server (NTRS)

Thigpen, William W.

2017-01-01

The NASA Advanced Supercomputing (NAS) Division enables advances in high-end computing technologies and in modeling and simulation methods to tackle some of the toughest science and engineering challenges facing NASA today. The name "NAS" has long been associated with leadership and innovation throughout the high-end computing (HEC) community. We play a significant role in shaping HEC standards and paradigms, and provide leadership in the areas of large-scale InfiniBand fabrics, Lustre open-source filesystems, and hyperwall technologies. We provide an integrated high-end computing environment to accelerate NASA missions and make revolutionary advances in science. Pleiades, a petaflop-scale supercomputer, is used by scientists throughout the U.S. to support NASA missions, and is ranked among the most powerful systems in the world. One of our key focus areas is in modeling and simulation to support NASA's real-world engineering applications and make fundamental advances in modeling and simulation methods.

Hydrological Modelling using HEC-HMS for Flood Risk Assessment of Segamat Town, Malaysia

NASA Astrophysics Data System (ADS)

Romali, N. S.; Yusop, Z.; Ismail, A. Z.

2018-03-01

This paper presents an assessment of the applicability of using Hydrologic Modelling System developed by the Hydrologic Engineering Center (HEC-HMS) for hydrological modelling of Segamat River. The objective of the model application is to assist in the assessment of flood risk by providing the peak flows of 2011 Segamat flood for the generation of flood mapping of Segamat town. The capability of the model was evaluated by comparing the historical observed data with the simulation results of the selected flood events. The model calibration and validation efficiency was verified using Nash-Sutcliffe model efficiency coefficient. The results demonstrate the interest to implement the hydrological model for assessing flood risk where the simulated peak flow result is in agreement with historical observed data. The model efficiency of the calibrated and validated exercises is 0.90 and 0.76 respectively, which is acceptable.

Impact of the Columbia Supercomputer on NASA Space and Exploration Mission

NASA Technical Reports Server (NTRS)

Biswas, Rupak; Kwak, Dochan; Kiris, Cetin; Lawrence, Scott

2006-01-01

NASA's 10,240-processor Columbia supercomputer gained worldwide recognition in 2004 for increasing the space agency's computing capability ten-fold, and enabling U.S. scientists and engineers to perform significant, breakthrough simulations. Columbia has amply demonstrated its capability to accelerate NASA's key missions, including space operations, exploration systems, science, and aeronautics. Columbia is part of an integrated high-end computing (HEC) environment comprised of massive storage and archive systems, high-speed networking, high-fidelity modeling and simulation tools, application performance optimization, and advanced data analysis and visualization. In this paper, we illustrate the impact Columbia is having on NASA's numerous space and exploration applications, such as the development of the Crew Exploration and Launch Vehicles (CEV/CLV), effects of long-duration human presence in space, and damage assessment and repair recommendations for remaining shuttle flights. We conclude by discussing HEC challenges that must be overcome to solve space-related science problems in the future.

The HEC RAS model of regulated stream for purposes of flood risk reduction

NASA Astrophysics Data System (ADS)

Fijko, Rastislav; Zeleňáková, Martina

2016-06-01

The work highlights the modeling of water flow in open channels using 1D mathematical model HEC-RAS in the area of interest Lopuchov village in eastern Slovakia. We created a digital model from a geodetic survey, which was used to show the area of inundation in ArcGIS software. We point out the modeling methodology with emphasis to collection of the data and their relevance for determination of boundary conditions in 3D model of the study area in GIS platform. The BIM objects can be exported to the defined model of the area. The obtained results were used for simulation of flooding. The results give to us clearly and distinctly defined areas of inundation, which we used in the processing of Cost benefit analysis. We used the developed model for stating the potential damages in flood vulnerable areas.

Selective attention impairments in Alzheimer's disease: evidence for dissociable components.

PubMed

Levinoff, Elise J; Li, Karen Z H; Murtha, Susan; Chertkow, Howard

2004-07-01

Tasks emphasizing 3 different aspects of selective attention-inhibition, visuospatial selective attention, and decision making-were administered to subjects with mild Alzheimer's disease (AD) and to healthy elderly control (HEC) subjects to determine which components of selective attention were impaired in AD subjects and whether selective attention could be dissociated into different components. The tasks were administered with easy versus hard levels of difficulty to assess proportional slowing as the key variable across tasks. The results indicated that the inhibitory and visual search tasks showed greater proportional slowing in subjects with AD than in HEC subjects, and that the task involving inhibition was significantly more affected in subjects with AD. Furthermore, there were no significant intertask correlations, and the results cannot be explained simply in terms of generalized cognitive slowing. These results provide evidence that inhibition is the most strikingly affected aspect of selective attention that is observed to be impaired in early stages of AD.

Synthesis and characterization of biopolymer based mixed matrix membranes for pervaporative dehydration.

PubMed

Das, Paramita; Ray, Samit Kumar

2014-03-15

Several blend membranes were prepared from different weight ratios of polyvinyl alcohol (PVA) and hydroxyethyl cellulose (HEC) and these unfilled membranes were crosslinked with maleic acid. In a similar way mixed matrix blend membranes were also prepared by varying weight ratio of PVA and HEC with micro and nano bentonite filler in each of these blends. These membranes were used for pervaporative dehydration of 89 wt% tetrahydrofuran (THF). Three membranes designated as UF (unfilled), MF2 (containing 2 wt% micro filler) and NF2 (containing 2 wt% nano filler) showing the best results for flux and selectivity were identified. These membranes were characterized by FTIR, UV, XRD and DTA-TG and used for separation of 80-99 wt% THF from water by pervaporation. The NF2 membrane was found to show the best results in terms of flux and separation factor. Copyright © 2013 Elsevier Ltd. All rights reserved.

The use and management of water in the Likangala Irrigation Scheme Complex in Southern Malawi

NASA Astrophysics Data System (ADS)

Mulwafu, Wapulumuka O.; Nkhoma, Bryson G.

This paper examines the uses and management of water for agriculture in Lake Chilwa catchment area in Zomba district of Southern Malawi. It focuses on the Likangala Rice Irrigation Scheme Complex situated along the Likangala River. The scheme is one of the largest government-run schemes. Established in the late 1960s by the government to meet the growing demand for rice, the scheme contributes greatly to the agricultural industry of the country. Besides, the scheme was established to ensure maximum utilization of Malawi's largest wetland, which, due to its hydromorphic soils and the littoral floodplains, does not favour the production of traditional upland seasonal crops such as maize. The scheme's overdependence on water from the Likangala River has attracted a considerable degree of academic interest in the use and management of the river to ensure that there is equity and efficiency for both productive and domestic users. The paper focuses on four main issues: the historical development of the scheme, the distribution of water to farmers, social relations, and the overall contribution of the scheme towards the social and economic development of the area and the country in general. The paper contends that the growing population of the basin and the increase in the number of formal and informal smallholder farmers, contributes greatly to the growth of competition and conflicts over water, which tends to undermine the economic potential of the scheme. Furthermore, the paper provides clearest indication of the need for a realistic and informed water management policy and strategy to solve the growing problem of social inequity without necessarily compromising the production of rice in the scheme.

78 FR 64916 - Circular Welded Carbon Steel Pipes and Tubes From Turkey: Final Results of Countervailing Duty...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-30

...: Land Allocation National Restructuring Program Regional Incentive Scheme: Reduced Corporate Tax Rates Regional Incentive Scheme: Social Security Premium Contribution for Employees Regional Incentive Scheme: Allocation of State Land Regional Incentive Scheme: Interest Support OIZ: Waste Water Charges OIZ: Exemptions...

Aurora A kinase contributes to a pole-based error correction pathway

PubMed Central

Ye, Anna A.; Deretic, Jovana; Hoel, Christopher M.; Hinman, Albert W.; Cimini, Daniela; Welburn, Julie P.; Maresca, Thomas J.

2015-01-01

Summary Chromosome biorientation, where sister kinetochores attach to microtubules (MTs) from opposing spindle poles, is the configuration that best ensures equal partitioning of the genome during cell division. Erroneous kinetochore-microtubule (kt-MT) attachments are commonplace but often corrected prior to anaphase [1, 2]. Error correction, thought to be mediated primarily by the centromere-enriched Aurora B kinase (ABK) [3-5], typically occurs near spindle poles [6]; albeit, the relevance of this locale is unclear. Furthermore, polar ejection forces (PEFs), highest near poles [7], can stabilize improper attachments by pushing mal-oriented chromosome arms away from spindle poles [8, 9]. Hence, a conundrum: erroneous kt-MT attachments are weakened where PEFs are most likely to strengthen them. Here, we report that Aurora A kinase (AAK) opposes the stabilizing effect of PEFs. AAK activity contributes to phosphorylation of kinetochore substrates near poles and its inhibition results in chromosome mis-alignment and an increased incidence of erroneous kt-MT attachments. Furthermore, AAK directly phosphorylates a site in the N-terminal tail of Ndc80/Hec1 that has been implicated in reducing the affinity of the Ndc80 complex for MTs when phosphorylated [10-12]. We propose that an AAK activity gradient contributes to correcting mal-oriented kt-MT attachments in the vicinity of spindle poles. PMID:26166783

Regulation of matriptase and HAI-1 system, a novel therapeutic target in human endometrial cancer cells.

PubMed

Sun, Pengming; Xue, Lifang; Song, Yiyi; Mao, Xiaodan; Chen, Lili; Dong, Binhua; Braicu, Elena Loana; Sehouli, Jalid

2018-02-27

The effects of specific and non-specific regulation of matriptase on endometrial cancer cells in vitro were investigated. Messenger ribonucleic acid (mRNA) and protein expression of matriptase and hepatocyte growth factor activator inhibitor-1 (HAI-1) in RL-952, HEC-1A, and HEC-1B endometrial cancer cells were detected by real-time quantitative PCR (RT-qPCR) and western blot. The cells were infected with lentivirus-mediated small-interfering RNA (siRNA) targeted on matriptase (MA-siRNA) or treated with different cisplatin (DDP) concentrations. After treatment, invasion, migration, and cellular apoptosis were analyzed. Matriptase mRNA and protein expression significantly decreased to 80% after infection with MA-siRNA ( P < 0.01), and scratch and trans-well chamber assays showed significant inhibition of invasiveness and metastasis. Upon incubation with cisplatin at concentrations higher than the therapeutic dose for 24 h, the expressions of matriptase and HAI-1 significantly decreased ( P < 0.001). Moreover, the invasiveness, metastasis, and survival rate of HEC-1A and RL-952 endometrial cancer cells were significantly decreased ( P < 0.001) due to the down-regulation of matriptase and HAI-1 upon increasing cisplatin concentration. However, a slight increase in matriptase and HAI-1 expression was observed in cells treated with low cisplatin concentration ( P = 0.01). Moreover, matriptase expression was associated with metastasis and invasiveness. Down-regulation of matriptase by specific Ma-SiRNA or non-specific cisplatin in matriptase/HAI-1-positive endometrial cancer cells showed promising therapeutic features.

Regulation of matriptase and HAI-1 system, a novel therapeutic target in human endometrial cancer cells

PubMed Central

Sun, Pengming; Xue, Lifang; Song, Yiyi; Mao, Xiaodan; Chen, Lili; Dong, Binhua; Braicu, Elena Loana; Sehouli, Jalid

2018-01-01

The effects of specific and non-specific regulation of matriptase on endometrial cancer cells in vitro were investigated. Messenger ribonucleic acid (mRNA) and protein expression of matriptase and hepatocyte growth factor activator inhibitor-1 (HAI-1) in RL-952, HEC-1A, and HEC-1B endometrial cancer cells were detected by real-time quantitative PCR (RT-qPCR) and western blot. The cells were infected with lentivirus-mediated small-interfering RNA (siRNA) targeted on matriptase (MA-siRNA) or treated with different cisplatin (DDP) concentrations. After treatment, invasion, migration, and cellular apoptosis were analyzed. Matriptase mRNA and protein expression significantly decreased to 80% after infection with MA-siRNA (P < 0.01), and scratch and trans-well chamber assays showed significant inhibition of invasiveness and metastasis. Upon incubation with cisplatin at concentrations higher than the therapeutic dose for 24 h, the expressions of matriptase and HAI-1 significantly decreased (P < 0.001). Moreover, the invasiveness, metastasis, and survival rate of HEC-1A and RL-952 endometrial cancer cells were significantly decreased (P < 0.001) due to the down-regulation of matriptase and HAI-1 upon increasing cisplatin concentration. However, a slight increase in matriptase and HAI-1 expression was observed in cells treated with low cisplatin concentration (P = 0.01). Moreover, matriptase expression was associated with metastasis and invasiveness. Down-regulation of matriptase by specific Ma-SiRNA or non-specific cisplatin in matriptase/HAI-1–positive endometrial cancer cells showed promising therapeutic features. PMID:29560101

A bipartite signal mediates the transfer of type IV secretion substrates of Bartonella henselae into human cells

PubMed Central

Schulein, Ralf; Guye, Patrick; Rhomberg, Thomas A.; Schmid, Michael C.; Schröder, Gunnar; Vergunst, Annette C.; Carena, Ilaria; Dehio, Christoph

2005-01-01

Bacterial type IV secretion (T4S) systems mediate the transfer of macromolecular substrates into various target cells, e.g., the conjugative transfer of DNA into bacteria or the transfer of virulence proteins into eukaryotic host cells. The T4S apparatus VirB of the vascular tumor-inducing pathogen Bartonella henselae causes subversion of human endothelial cell (HEC) function. Here we report the identification of multiple protein substrates of VirB, which, upon translocation into HEC, mediate all known VirB-dependent cellular changes. These Bartonella-translocated effector proteins (Beps) A-G are encoded together with the VirB system and the T4S coupling protein VirD4 on a Bartonella-specific pathogenicity island. The Beps display a modular architecture, suggesting an evolution by extensive domain duplication and reshuffling. The C terminus of each Bep harbors at least one copy of the Bep-intracellular delivery domain and a short positively charged tail sequence. This biparte C terminus constitutes a transfer signal that is sufficient to mediate VirB/VirD4-dependent intracellular delivery of reporter protein fusions. The Bep-intracellular delivery domain is also present in conjugative relaxases of bacterial conjugation systems. We exemplarily show that the C terminus of such a conjugative relaxase mediates protein transfer through the Bartonella henselae VirB/VirD4 system into HEC. Conjugative relaxases may thus represent the evolutionary origin of the here defined T4S signal for protein transfer into human cells. PMID:15642951

Physical and Cognitive Stimulation Using an Exergame in Subjects with Normal Aging, Mild and Moderate Cognitive Impairment.

PubMed

Ben-Sadoun, Grégory; Sacco, Guillaume; Manera, Valeria; Bourgeois, Jérémy; König, Alexandra; Foulon, Pierre; Fosty, Baptiste; Bremond, François; d'Arripe-Longueville, Fabienne; Robert, Philippe

2016-06-30

The use of Serious exerGames (SeG) as enriched environments (EE), which promotes cognitive simulation with physical activity in a positive emotional context, has been proposed to represent a powerful method to slow down the decline due to neurodegenerative diseases (ND), such as Alzheimer's disease (AD). However, so far, no SeG targeting EE has been tested in ND subjects. This study aimed at evaluating the usability and short-term training effects of X-Torp, an action SeG designed for elderly ND subjects with mild cognitive impairment (MCI) and AD. X-Torp is a SeG played using the Microsoft® Kinect™. 10 ND subjects and 8 healthy elderly controls (HEC) were enrolled in a 1-month program with three training sessions per week. Usability was evaluated through game time, game performance, the aerobic intensity level reached, perceived emotions, and perceived usability. All participants successfully completed the training program. ND subjects played less and had a lower game performance compared to HEC. During the sessions, ND subjects maintained a light intensity of aerobic activity, while HEC maintained a moderate intensity. Both groups experienced only positive emotions, and reported a 'moderate' to 'high' perceived competence, a 'moderate' game difficulty, and a 'high' interest in the game. Usability results suggest that X-Torp represents a usable EE for healthy subjects and persons with MCI and AD. However, in order to reach moderate or high intensity of aerobic activity, X-Torp control modes should be adapted to become more physically stimulating.

Development of swelling/floating gastroretentive drug delivery system based on a combination of hydroxyethyl cellulose and sodium carboxymethyl cellulose for Losartan and its clinical relevance in healthy volunteers with CYP2C9 polymorphism.

PubMed

Chen, Ray-Neng; Ho, Hsiu-O; Yu, Chiao-Ya; Sheu, Ming-Thau

2010-01-31

The aim of this study was to develop an optimal gastroretentive drug delivery system (GRDDS) for administering Losartan. Additionally, the influence of optimized GRDDS on the bioavailability of Losartan and the formation extent of active metabolite E3174 by CYP2C9 polymorphism was investigated. Swellable and floatable GRDDS tablets combining hydroxyethyl cellulose (HEC), sodium carboxymethyl cellulose (NaCMC), and sodium bicarbonate were prepared at various compression pressures for evaluating swelling characteristics and floating capacity. Then Losartan was incorporated into optimized formulations for in vitro and in vivo characterizations. An appropriate ratio of HEC to NaCMC, addition of sodium bicarbonate, and compression at lower pressures resulted in the tablets floating over SGF for more than 16 h and swelling to 2 cm in diameter within 3h. The release patterns of Losartan from these tablets were pH-dependent. Results of the clinical trials showed that the mean bioavailability from GRD-A (HEC 91.67%, sodium bicarbonate 3.33% and Losartan 8.33%) was approximately 164%, relative to the immediate-release product (Cozaar). MRT and t(max) values were greater and C(max) values were lower for the GRDDS tablets compared with Cozaa. The lower bioavailability of Losartan in the CYP2C9*1/*1 subjects than CYP2C9*1/*3 subjects was found and could be due to the variety of enzymatic activity. Copyright 2009 Elsevier B.V. All rights reserved.

An Ethylene-Protected Achilles’ Heel of Etiolated Seedlings for Arthropod Deterrence

PubMed Central

Boex-Fontvieille, Edouard; Rustgi, Sachin; von Wettstein, Diter; Pollmann, Stephan; Reinbothe, Steffen; Reinbothe, Christiane

2016-01-01

A small family of Kunitz protease inhibitors exists in Arabidopsis thaliana, a member of which (encoded by At1g72290) accomplishes highly specific roles during plant development. Arabidopsis Kunitz-protease inhibitor 1 (Kunitz-PI;1), as we dubbed this protein here, is operative as cysteine PI. Activity measurements revealed that despite the presence of the conserved Kunitz-motif the bacterially expressed Kunitz-PI;1 was unable to inhibit serine proteases such as trypsin and chymotrypsin, but very efficiently inhibited the cysteine protease RESPONSIVE TO DESICCATION 21. Western blotting and cytolocalization studies using mono-specific antibodies recalled Kunitz-PI;1 protein expression in flowers, young siliques and etiolated seedlings. In dark-grown seedlings, maximum Kunitz-PI;1 promoter activity was detected in the apical hook region and apical parts of the hypocotyls. Immunolocalization confirmed Kunitz-PI;1 expression in these organs and tissues. No transmitting tract (NTT) and HECATE 1 (HEC1), two transcription factors previously implicated in the formation of the female reproductive tract in flowers of Arabidopsis, were identified to regulate Kunitz-PI;1 expression in the dark and during greening, with NTT acting negatively and HEC1 acting positively. Laboratory feeding experiments with isopod crustaceans such as Porcellio scaber (woodlouse) and Armadillidium vulgare (pillbug) pinpointed the apical hook as ethylene-protected Achilles’ heel of etiolated seedlings. Because exogenous application of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) and mechanical stress (wounding) strongly up-regulated HEC1-dependent Kunitz-PI;1 gene expression, our results identify a new circuit controlling herbivore deterrence of etiolated plants in which Kunitz-PI;1 is involved. PMID:27625656

Differences in Chlamydia trachomatis Serovar E Growth Rate in Polarized Endometrial and Endocervical Epithelial Cells Grown in Three-Dimensional Culture▿

PubMed Central

Guseva, Natalia V.; Dessus-Babus, Sophie; Moore, Cheryl G.; Whittimore, Judy D.; Wyrick, Priscilla B.

2007-01-01

In vitro studies of obligate intracellular chlamydia biology and pathogenesis are highly dependent on the use of experimental models and growth conditions that mimic the mucosal architecture and environment these pathogens encounter during natural infections. In this study, the growth of Chlamydia trachomatis genital serovar E was monitored in mouse fibroblast McCoy cells and compared to more relevant host human epithelial endometrium-derived HEC-1B and cervix-derived HeLa cells, seeded and polarized on collagen-coated microcarrier beads, using a three-dimensional culture system. Microscopy analysis of these cell lines prior to infection revealed morphological differences reminiscent of their in vivo architecture. Upon infection, early chlamydial inclusion distribution was uniform in McCoy cells but patchy in both epithelial cell lines. Although no difference in chlamydial attachment to or entry into the two genital epithelial cell lines was noted, active bacterial genome replication and transcription, as well as initial transformation of elementary bodies to reticulate bodies, were detected earlier in HEC-1B than in HeLa cells, suggesting a faster growth, which led to higher progeny counts and titers in HEC-1B cells upon completion of the developmental cycle. Chlamydial development in the less relevant McCoy cells was very similar to that in HeLa cells, although higher progeny counts were obtained. In conclusion, this three-dimensional bead culture system represents an improved model for harvesting large quantities of infectious chlamydia progeny from their more natural polarized epithelial host cells. PMID:17088348

[Aromatase activities of endometrial carcinomas and both basic and clinical analyses of endometrial hyperplasia as a premalignant disease].

PubMed

Sasaki, H

1993-08-01

Paraffin-embedded materials obtained from 117 cases of endometrial hyperplasia and 84 cases of carcinoma were used for measurement of both ki-ras and p53 gene mutation and aromatase (ARO) and TGF-alpha immunostaining. The overall incidence of ki-ras mutations in the hyperplasia specimens (16%) was similar to the incidence detected in carcinomas (18%). None of 117 endometrial hyperplasias were found to have mutations in the p53 gene, whereas mutations were seen in 3 (13.3%) endometrial carcinomas. The intensity of both ARO and TGF-alpha immunostaining was increased in glands of both hyperplasia and carcinoma, and also in the interstitium of carcinoma. The positive sites of both ARO and TGF-alpha were almost the same, with an incidence below 40% in both hyperplasias and carcinomas. The cultured cells of endometrial carcinoma showed aromatase activity below MCF-7 cells, because testosterone was converted to estradiol (E2). TGF-alpha induced cell growth with at an optimal concentration. In HEC-59 cells, TGF-alpha increased both ARO-activity and mRNA. Some promoters on ARO-exon 1 in HEC-59 cells were different from those in BeWo cells. Progesterone inhibited the E2-induced excretion of pre TGF-alpha in endometrial carcinoma cells. These findings suggest that endometrial hyperplasia can be a premalignant condition of carcinoma, and can be initiated by both ki-ras codon 12 mutation and abnormal activity of ARO induced by TGF-alpha. In addition, HEC-59 cells may possess autocrine/paracrine properties involving ARO, E2 and TGF-alpha.

[The factors involved in invasive ability of endometrial carcinoma cells].

PubMed

Mori, Y; Mizuuchi, H; Sato, K; Okamura, N; Kudo, R

1994-06-01

The in vitro invasive ability, the expression of cell adhesion molecule E-cadherin, activity of matrix metalloproteinase (MMP) and K-ras point mutation were investigated in eight human endometrial carcinoma cell lines. 1) In vitro invasive abilities of endometrial carcinoma cell lines depend on the degree of cell differentiation and the origin of cell lines. A poorly-differentiated carcinoma cell line (NUE-1) and a cell line derived from metastatic lymph node (SNG-M) were more invasive than moderately-(HEC-1A, HEC-1BE) and well-differentiated (HEC-6, Ishikawa) cell lines. 2) Immunohistochemically, less or non-invasive cell lines expressed E-cadherin strongly, whereas a highly invasive cell line (NUE-1) expressed E-cadherin weakly. 3) When cultured on Matrigel-coated dishes, the tumor cells derived from moderately- and well-differentiated carcinoma aggregated with each other and did not invade Matrigel in the invasion assay. The aggregated cells expressed E-cadherin more strongly when cultured on Matrigel. 4) 72-kD gelatinase (MMP-2) was secreted in serum-free conditioned medium of all cell lines. In an invasive cell line (NUE-1,SNG-M), the activity of MMP-2 was stronger than in other cell lines. And the activity of 92-kDa gelatinase (MMP-9) was detected in most invasive cell line (NUE-1). 5) Point mutation of K-ras codon 12 was detected in four of eight (50%) cell lines by the PCR-RFLP method. The changes in the DNA sequence were identified, but K-ras point mutation was not correlated with in vitro invasiveness of the tumor cells.

Microarray pathway analysis indicated that mitogen-activated protein kinase/extracellular signal-regulated kinase and insulin growth factor 1 signaling pathways were inhibited by small interfering RNA against AT-rich interactive domain 1A in endometrial cancer

PubMed Central

Yang, Ye; Bao, Wei; Sang, Zhengyu; Yang, Yongbing; Lu, Meng; Xi, Xiaowei

2018-01-01

Mutations in the gene encoding AT-rich interactive domain 1A (ARID1A) are frequently observed in endometrial cancer (EC) but the molecular mechanisms linking the genetic changes remain to be fully understood. The present study aimed to elucidate the influence of ARID1A mutations on signaling pathways. Missense, synonymous and nonsense heterozygous ARID1A mutations in the EC HEC-1-A cell line were verified by Sanger sequencing. Mutated ARID1A small interfering RNA was transfected into HEC-1-A cells. Biochemical microarray analysis revealed 13 upregulated pathways, 17 downregulated pathways, 14 significantly affected disease states and functions, 662 upstream and 512 downstream genes in mutated ARID1A-depleted HEC-1-A cells, among which the mitogen-activated protein kinase/extracellular signal-regulated kinase and insulin-like growth factor-1 (IGF1) signaling pathways were the 2 most downregulated pathways. Furthermore, the forkhead box protein O1 pathway was upregulated, while the IGF1 receptor, insulin receptor substrate 1 and phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit b pathways were downregulated. Carcinoma tumorigenesis, tumor cell mitosis and tumor cell death were significantly upregulated disease states and functions, while cell proliferation and tumor growth were significantly downregulated. The results of the present study suggested that ARID1A may be a potential prognostic and therapeutic molecular drug target for the prevention of EC progression. PMID:29399196

Association of physical performance and biochemical profile of mice with intrinsic endurance swimming.

PubMed

Huang, Wen-Ching; Hsu, Yi-Ju; Wei, Li; Chen, Ying-Ju; Huang, Chi-Chang

2016-01-01

We aimed to investigate the potential mediators and relationship affecting congenital exercise performance in an animal model with physical activity challenge from physiological and biochemical perspectives. A total of 75 male ICR mice (5 weeks old) were adapted for 1 week, then mice performed a non-loading and exhaustive swimming test and were assigned to 3 groups by exhaustive swimming time: low exercise capacity (LEC) (<3 hr), medium exercise capacity (MEC) (3-5 hr), and high exercise capacity (HEC) (>5 hr). After a 1-week rest, the 3 groups of mice performed an exhaustive swimming test with a 5% and 7.5% weight load and a forelimb grip-strength test, with a 1-week rest between tests. Blood samples were collected immediately after an acute exercise challenge and at the end of the experiment (resting status) to evaluate biochemical blood variables and their relation with physical performance. Physical activity, including exhaustive swimming and grip strength, was greater for HEC than other mice. The swimming performance and grip strength between groups were moderately correlated (r=0.443, p <0.05). Resting serum ammonium level was moderately correlated with endurance with a 7.5% weight load (r=-0.447, p <0.05) and with lactate level (r=0.598, p <0.05). The pulmonary morphology of the HEC group seemed to indicate benefits for aerobic exercise. Mice showed congenital exercise performance, which was significantly correlated with different physical challenges and biochemical variable values. This study may have implications for interference in intrinsic characteristics.

Production of light elements and 98Tc through the ν-process with the neutrino oscillation in supernova explosion

NASA Astrophysics Data System (ADS)

Ko, Heamin; Kusakabe, Motohiko; Cheoun, Myung-Ki; Kwak, Kyujin; Kim, Kyungsik

2018-04-01

Stars ending with core collapse supernovae (SNe) emit a tremendous number of neutrinos during their explosions. While these neutrinos pass through each layer of the stars, they react with the nuclides in the progenitor. Although the neutrino cross sections are very small, its huge flux is high enough to react with nuclides to change their abundances. We consider this ν-process, by which abundances of some elements may be explained exclusively by this neutrino process. One of the candidates is 98Tc. In this study, we check the ν-process contributions due to the neutrino reactions. In this calculation we also include the neutrino oscillation effect in the matter with varying density, so called MSW effect, not only for 98Tc production but also 4He and 12C destruction, which occurs at first in the He/C layer in the present model.

Evaluating scour at bridges.

DOT National Transportation Integrated Search

1995-11-01

This document is the third of HEC 18, i.e., presents the state of knowledge and practice for the design, : evaluation, and inspection of bridges for scour. It contains updated material not included in the second : edition dated April 1993. This docum...

A model study of bridge hydraulics : technical summary.

DOT National Transportation Integrated Search

2010-08-01

Most flood studies in the United States use the Army Corps of Engineers Hydrologic Engineering Centers River Analysis System (HEC-RAS) computer program. This report is the second edition. The first edition of the report considered the laboratory m...

A Comparison of Different Slicing Planes in Preservation of Major Hippocampal Pathway Fibers in the Mouse

PubMed Central

Xiong, Guoxiang; Metheny, Hannah; Johnson, Brian N.; Cohen, Akiva S.

2017-01-01

The hippocampus plays a critical role in learning and memory and higher cognitive functions, and its dysfunction has been implicated in various neuropathological disorders. Electrophysiological recording undertaken in live brain slices is one of the most powerful tools for investigating hippocampal cellular and network activities. The plane for cutting the slices determines which afferent and/or efferent connections are best preserved, and there are three commonly used slices: hippocampal-entorhinal cortex (HEC), coronal and transverse. All three slices have been widely used for studying the major afferent hippocampal pathways including the perforant path (PP), the mossy fibers (MFs) and the Schaffer collaterals (SCs). Surprisingly, there has never been a systematic investigation of the anatomical and functional consequences of slicing at a particular angle. In the present study, we focused on how well fiber pathways are preserved from the entorhinal cortex (EC) to the hippocampus, and within the hippocampus, in slices generated by sectioning at different angles. The postmortem neural tract tracer 1,1′-dioctadecyl-3,3,3′3′-tetramethylindocarbocyanine perchlorate (DiI) was used to label afferent fibers to hippocampal principal neurons in fixed slices or whole brains. Laser scanning confocal microscopy was adopted for imaging DiI-labeled axons and terminals. We demonstrated that PP fibers were well preserved in HEC slices, MFs in both HEC and transverse slices and SCs in all three types of slices. Correspondingly, field excitatory postsynaptic potentials (fEPSPs) could be consistently evoked in HEC slices when stimulating PP fibers and recorded in stratum lacunosum-moleculare (sl-m) of area CA1, and when stimulating the dentate granule cell layer (gcl) and recording in stratum lucidum (sl) of area CA3. The MF evoked fEPSPs could not be recorded in CA3 from coronal slices. In contrast to our DiI-tracing data demonstrating severely truncated PP fibers in coronal slices, fEPSPs could still be recorded in CA1 sl-m in this plane, suggesting that an additional afferent fiber pathway other than PP might be involved. The present study increases our understanding of which hippocampal pathways are best preserved in the three most common brain slice preparations, and will help investigators determine the appropriate slices to use for physiological studies depending on the subregion of interest. PMID:29201002

Corps Water Management System (CWMS) Decision Support Modeling and Integration Use in the June 2007 Texas Floods

NASA Astrophysics Data System (ADS)

Charley, W. J.; Luna, M.

2007-12-01

The U.S. Army Corps of Engineers Corps Water Management System (CWMS) is a comprehensive data acquisition and hydrologic modeling system for short-term decision support of water control operations in real time. It encompasses data collection, validation and transformation, data storage, visualization, real time model simulation for decision-making support, and data dissemination. CWMS uses an Oracle database and Sun Solaris workstations for data processes, storage and the execution of models, with a client application (the Control and Visualization Interface, or CAVI) that can run on a Windows PC. CWMS was used by the Lower Colorado River Authority (LCRA) to make hydrologic forecasts of flows on the Lower Colorado River and operate reservoirs during the June 2007 event in Texas. The LCRA receives real-time observed gridded spatial rainfall data from OneRain, Inc. that which is a result of adjusting NexRad rainfall data with precipitation gages. This data is used, along with future precipitation estimates, for hydrologic forecasting by the rainfall-runoff modeling program HEC-HMS. Forecasted flows from HEC-HMS and combined with observed flows and reservoir information to simulate LCRA's reservoir operations and help engineers make release decisions based on the results. The river hydraulics program, HEC-RAS, computes river stages and water surface profiles for the computed flow. An inundation boundary and depth map of water in the flood plain can be calculated from the HEC-RAS results using ArcInfo. By varying future precipitation and releases, engineers can evaluate different "What if?" scenarios. What was described as an "extraordinary cluster of thunderstorms" that stalled over Burnet and Llano counties in Texas on June 27, 2007, dropped 17 to 19 inches of rainfall over a 6-hour period. The storm was classified over a 500-year event and the resulting flow over some of the smaller tributaries as a 100-year or better. CWMS was used by LCRA for flood forecasting and reservoir operations. The models accurately forecasting the flows and allowed engineers to determine that only four floodgates needed to be opened for Mansfield dam, in the Chain of Highland lakes. CWMS also forecasted the peak of the flood well before it happened. Smaller rain storms continued for a period of weeks and CWMS was used throughout the event calculating lake levels, closing of gates along with a hydro-generation schedule.

Anti-tumor activity of olaparib, a poly (ADP-ribose) polymerase (PARP) inhibitor, in cultured endometrial carcinoma cells

PubMed Central

2014-01-01

Background PTEN inactivation is the most frequent genetic aberration in endometrial cancer. One of the phosphatase-independent roles of PTEN is associated with homologous recombination (HR) in nucleus. Poly (ADP-ribose) polymerase (PARP) plays key roles in the repair of DNA single-strand breaks, and a PARP inhibitor induces synthetic lethality in cancer cells with HR deficiency. We examined the anti-tumor activity of olaparib, a PARP inhibitor, and its correlation between the sensitivity and status of PTEN in endometrial cancer cell lines. Methods The response to olaparib was evaluated using a clonogenic assay with SF50 values (concentration to inhibit cell survival to 50%) in 16 endometrial cancer cell lines. The effects of PTEN on the sensitivity to olaparib and ionizing radiation (IR) exposure were compared between parental HEC-6 (PTEN-null) and HEC-6 PTEN + (stably expressing wild-type PTEN) cells by clonogenic assay, foci formation of RAD51 and γH2AX, and induction of cleaved PARP. The effects of siRNA to PTEN were analyzed in cells with wild-type PTEN. Results The SF50 values were 100 nM or less in four (25%: sensitive) cell lines; whereas, SF50 values were 1,000 nM or more in four (25%: resistant) cell lines. PTEN mutations were not associated with sensitivity to olaparib (Mutant [n = 12]: 746 ± 838 nM; Wild-type [n = 4]: 215 ± 85 nM, p = 0.26 by Student’s t test). RAD51 expression was observed broadly and was not associated with PTEN status in the 16 cell lines. The number of colonies in the clonogenic assay, the foci formation of RAD51 and γH2AX, and the induction of apoptosis were not affected by PTEN introduction in the HEC-6 PTEN + cells. The expression level of nuclear PTEN was not elevated within 24 h following IR in the HEC-6-PTEN + cells. In addition, knocking down PTEN by siRNA did not alter the sensitivity to olaparib in 2 cell lines with wild-type PTEN. Conclusions Our results suggest that olaparib, a PARP inhibitor, is effective on certain endometrial cancer cell lines. Inactivation of PTEN might not affect the DNA repair function. Predictive biomarkers are warranted to utilize olaparib in endometrial cancer. PMID:24625059

Characterizing coarse bedload transport during floods with RFID and accelerometer tracers, in-stream RFID antennas and HEC-RAS modeling

NASA Astrophysics Data System (ADS)

Olinde, L.; Johnson, J. P.

2013-12-01

By monitoring the transport timing and distances of tracer grains in a steep mountains stream, we collected data that can constrain numerical bedload transport models considered for these systems. We captured bedload activity during a weeks-spanning snowmelt period in Reynolds Creek, Idaho by deploying Radio Frequency Identification (RFID) and accelerometer embedded tracers with in-stream stationary RFID antennas. During transport events, RFID dataloggers recorded the times when tracers passed over stationary antennas. The accelerometer tracers also logged x, y, z-axis accelerations every 10 minutes to identify times of motion and rest. After snowmelt flows receded, we found tracers with mobile antennas and surveyed their positions. We know the timing and tracer locations when accelerometer tracers were initially entrained, passed stationary antennas, and were finally deposited at the surveyed locations. The fraction of moving accelerometers over time correlates well with discharge. Comparisons of the transported tracer fraction between rising and falling limbs over multiple flood peaks suggest that some degree of clockwise hysteresis persisted during the snowmelt period. Additionally, we apply accelerometer transport durations and displacement distances to calculate virtual velocities over full tracer path lengths and over lengths between initial locations to stationary antennas as well as between stationary antennas to final positions. The accelerometer-based virtual velocities are significantly faster than those estimated from traditional tracer methods that estimate bedload transport durations by assuming threshold flow conditions. We also subsample the motion data to calculate how virtual velocities change over the measurement intervals. Regressions of these relations are in turn used to extrapolate virtual velocities at smaller sampling timescales. Minimum hop lengths are also evaluated for each accelerometer tracer. Finally, flow conditions during the snowmelt hydrograph are modeled over the 11 kilometers of surveyed stream by utilizing 1m airborne LiDAR and HEC-GeoRAS. Cross-sectional HEC-RAS results are used to estimate the spatial distribution of longitudinal shear velocities over the observed discharges. At final accelerometer tracer positions, we analyze the HEC-RAS generated flow conditions for each disentrainment discharge magnitude. The techniques developed here have the potential to link individual grain characteristics during floods to a range of time and length scales.

77 FR 46713 - Circular Welded Carbon Steel Pipes and Tubes From Turkey: Final Results of Countervailing Duty...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-06

... Support. L. IEP: Land Allocation. M. National Restructuring Program. N. Regional Incentive Scheme: Reduced Corporate Tax Rates. O. Regional Incentive Scheme: Social Security Premium Contribution for Employees. P. Regional Incentive Scheme: Allocation of State Land. Q. Regional Incentive Scheme: Interest Support. R. OIZ...

Final Scientific/Technical Report for "Enabling Exascale Hardware and Software Design through Scalable System Virtualization"

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dinda, Peter August

2015-03-17

This report describes the activities, findings, and products of the Northwestern University component of the "Enabling Exascale Hardware and Software Design through Scalable System Virtualization" project. The purpose of this project has been to extend the state of the art of systems software for high-end computing (HEC) platforms, and to use systems software to better enable the evaluation of potential future HEC platforms, for example exascale platforms. Such platforms, and their systems software, have the goal of providing scientific computation at new scales, thus enabling new research in the physical sciences and engineering. Over time, the innovations in systems softwaremore » for such platforms also become applicable to more widely used computing clusters, data centers, and clouds. This was a five-institution project, centered on the Palacios virtual machine monitor (VMM) systems software, a project begun at Northwestern, and originally developed in a previous collaboration between Northwestern University and the University of New Mexico. In this project, Northwestern (including via our subcontract to the University of Pittsburgh) contributed to the continued development of Palacios, along with other team members. We took the leadership role in (1) continued extension of support for emerging Intel and AMD hardware, (2) integration and performance enhancement of overlay networking, (3) connectivity with architectural simulation, (4) binary translation, and (5) support for modern Non-Uniform Memory Access (NUMA) hosts and guests. We also took a supporting role in support for specialized hardware for I/O virtualization, profiling, configurability, and integration with configuration tools. The efforts we led (1-5) were largely successful and executed as expected, with code and papers resulting from them. The project demonstrated the feasibility of a virtualization layer for HEC computing, similar to such layers for cloud or datacenter computing. For effort (3), although a prototype connecting Palacios with the GEM5 architectural simulator was demonstrated, our conclusion was that such a platform was less useful for design space exploration than anticipated due to inherent complexity of the connection between the instruction set architecture level and the microarchitectural level. For effort (4), we found that a code injection approach proved to be more fruitful. The results of our efforts are publicly available in the open source Palacios codebase and published papers, all of which are available from the project web site, v3vee.org. Palacios is currently one of the two codebases (the other being Sandia’s Kitten lightweight kernel) that underlies the node operating system for the DOE Hobbes Project, one of two projects tasked with building a systems software prototype for the national exascale computing effort.« less

Determination of rainfall losses in Virginia : the effects of urbanization.

DOT National Transportation Integrated Search

1983-01-01

The effects of urbanization on the Corps of Engineers' HEC-I rainfall-runoff model parameters were examined. Data on rainfall events and corresponding streamflow hydrographs were gathered for five watersheds in rural and highly urbanized areas in Vir...

HEC-RAS 2.2 for backwater and scour analysis - phase one

DOT National Transportation Integrated Search

2000-09-01

The Kansas Department of Transportation (KDOT) and most bridge consultants in Kansas have been using the DOS-WSPRO program and the KDOT scour spreadsheets to perform bridge hydraulics and scour analysis for the past several years. Unfortunately, DOS-...

Swift fox survey along Heartland Expressway Corridor.

DOT National Transportation Integrated Search

2015-05-01

The swift fox (Vulpes velox) is a small canid classified as endangered within the : state of Nebraska. Future construction of the Heartland Expressway Corridor (HEC), a : 300 km road expansion project in the panhandle of the state, may impact the res...

Updating HEC-18 pier scour equations for noncohesive soils.

DOT National Transportation Integrated Search

2016-10-01

A dataset of 594 bridge pier scour observations from two laboratory and three field studies was compiled. The dataset served as the testing ground for evaluating potential enhancements to the pier scour tools for noncohesive soils in Hydraulic Engine...

Developing a contributing factor classification scheme for Rasmussen's AcciMap: Reliability and validity evaluation.

PubMed

Goode, N; Salmon, P M; Taylor, N Z; Lenné, M G; Finch, C F

2017-10-01

One factor potentially limiting the uptake of Rasmussen's (1997) Accimap method by practitioners is the lack of a contributing factor classification scheme to guide accident analyses. This article evaluates the intra- and inter-rater reliability and criterion-referenced validity of a classification scheme developed to support the use of Accimap by led outdoor activity (LOA) practitioners. The classification scheme has two levels: the system level describes the actors, artefacts and activity context in terms of 14 codes; the descriptor level breaks the system level codes down into 107 specific contributing factors. The study involved 11 LOA practitioners using the scheme on two separate occasions to code a pre-determined list of contributing factors identified from four incident reports. Criterion-referenced validity was assessed by comparing the codes selected by LOA practitioners to those selected by the method creators. Mean intra-rater reliability scores at the system (M = 83.6%) and descriptor (M = 74%) levels were acceptable. Mean inter-rater reliability scores were not consistently acceptable for both coding attempts at the system level (M T1  = 68.8%; M T2  = 73.9%), and were poor at the descriptor level (M T1  = 58.5%; M T2  = 64.1%). Mean criterion referenced validity scores at the system level were acceptable (M T1  = 73.9%; M T2  = 75.3%). However, they were not consistently acceptable at the descriptor level (M T1  = 67.6%; M T2  = 70.8%). Overall, the results indicate that the classification scheme does not currently satisfy reliability and validity requirements, and that further work is required. The implications for the design and development of contributing factors classification schemes are discussed. Copyright © 2017 Elsevier Ltd. All rights reserved.

Evaluation of design methods to determine scour depths for bridge structures : [technical summary].

DOT National Transportation Integrated Search

2013-01-01

Scour of bridge foundations is the most common cause of bridge failures. The Federal Highway Administration : (FHWA) has developed a design method, HEC-18, for the state Departments of Transportation (DOTs) to evaluate : the scour potential of existi...

Evaluation of design methods to determine scour depths for bridge structures.

DOT National Transportation Integrated Search

2013-03-01

Scour of bridge foundations is the most common cause of bridge failures. The overall goal of this project was to evaluate the applicability of the existing Hydraulic Engineering Circular (HEC-18) documents method to Louisiana bridges that are mostly ...

Chattahoochee River Water Quality Analysis.

DTIC Science & Technology

1978-04-01

in 1972 with WRE for addition of storm water quality computations. Since then the HEC has added other capabilities including snowmelt and land surface...Geological Survey. The storm water quality data were reported in reference 2. Quantity The quantity calibration involved adjusting the pervious area

Design and Evaluation of Scour for Bridges Using HEC-18 : technical brief.

DOT National Transportation Integrated Search

2017-07-01

This project developed a new approach for evaluating erosive scour at New Jersey bridges over non-tidal waterways. The main deliverable was the Scour Evaluation Model (SEM), which offers new analysis procedures, while still retaining the applicable p...

Public and Private Hospital Nurses’ Perceptions of the Ethical Climate in Their Work Settings, Sari City, 2011

PubMed Central

Ghorbani, Ali Asghar; Hesamzadeh, Ali; Khademloo, Mohammad; Khalili, Salimeh; Hesamzadeh, Shamim; Berger, Valerie

2014-01-01

Background: Nurses’ perceptions of ethical climate patterns have certain undeniable effects on hospitals. There is little evidence of possible differences in this element between public and private hospitals and contributing factors. Objectives: This study investigated whether the perceptions of the ethical climate in nurses’ working in public hospitals differ from that of nurses in private hospitals, and which factors may affect nurses’ perceptions. Materials and Methods: A cross-sectional study of randomly selected registered nurses (n = 235), working in four public hospitals affiliated to Mazandaran University of Medical Sciences, and three private hospitals, was conducted in Sari City, Iran. A self-administered questionnaire, containing demographic characteristics and the Hospital Ethical Climate Survey (HECS), were used to assess registered nurses’ perceptions of public and private hospitals ethical climate. An independent t-test and one-way ANOVA were used to analyze the data. Results: Across the five factors of HECS, the highest and lowest mean scores pertained to managers and physicians, respectively, in both public and private hospitals. Nurses who had a conditional employment situation and those working in pediatric intensive care units showed significantly more positive perceptions of the ethical work climate when compared to their peers (P < 0.05). Although the mean score of ethical work climate in private hospitals (3.82 ± 0.61) was higher than that in public hospitals (3.76 ± 0.54), no significant difference was found (P = 0.44). Conclusions: Hospital managers need to discover better ways to promote safety and health programs for their staff according to nurses’ area of work and their type of units. They should also encourage greater levels of participation in safety-enhancing initiatives in the hospital’s ethical climate, especially in the areas of nurses’ perceptions of their physician colleagues, and for nurses with a conditional employment situation. PMID:25414890

Thermal and Hydraulic Conditions Supporting the Recruitment of Asian Carp in Seiche Affected Rivers of Lake Erie Basin - A Case Study of the Lower Sandusky River in Ohio

NASA Astrophysics Data System (ADS)

Soong, D. T.; Santacruz, S.; Jones, L.; Garcia, T.; Kočovský, P. M.; Embke, H.

2017-12-01

Grass Carp Ctenopharyngodon idella (Cyprinidae) is an invasive fish species that spawns in rivers during high-flow events. In their native range, it is believed eggs must hatch within the riverine environment in order to eventually result in production of adult fish. The lower Sandusky River is approximately 26 km long extending from its confluence with Sandusky Bay upstream to the Ballville Dam, which is impassible for Grass Carp. Grass Carp are known to have spawned in the Sandusky River, a tributary to Lake Erie, in 2011, 2013, 2015, and 2017. This study characterizes the thermal and hydraulic conditions under which these eggs could hatch in the lower Sandusky River, a relatively short river reach for egg hatching. Grass Carp eggs collected in 2015 were previously analyzed for hatching locations using a one-dimensional steady-state HEC-RAS hydraulic model. In this study we refine estimates of hatching locations by incorporating the influence of fluctuating water levels downstream due to seiches in Lake Erie and overland and tributary inflows using an unsteady 1D/2D HEC-RAS hydraulic model. Additionally, conditions conducive to successful hatching, which occurs when eggs reach the hatching stage within the river, were analyzed from nine high-flow events between 2011 and 2015. Simulated hydraulic and water temperature data were used as inputs to the Fluvial Egg Drift Simulator (FluEgg) model, which was used to analyze the transport and dispersal of Grass carp eggs until hatching. We will describe the differences in steady- and unsteady-state hydraulic modeling in predicting hatching locations of Grass Carp eggs for the 2015 spawning events. Results will also include hydraulic and temperature variables that contribute to the successful/unsuccessful in-river hatching for the nine flow events simulated.

Event-based rainfall-runoff modelling of the Kelantan River Basin

NASA Astrophysics Data System (ADS)

Basarudin, Z.; Adnan, N. A.; Latif, A. R. A.; Tahir, W.; Syafiqah, N.

2014-02-01

Flood is one of the most common natural disasters in Malaysia. According to hydrologists there are many causes that contribute to flood events. The two most dominant factors are the meteorology factor (i.e climate change) and change in land use. These two factors contributed to floods in recent decade especially in the monsoonal catchment such as Malaysia. This paper intends to quantify the influence of rainfall during extreme rainfall events on the hydrological model in the Kelantan River catchment. Therefore, two dynamic inputs were used in the study: rainfall and river discharge. The extreme flood events in 2008 and 2004 were compared based on rainfall data for both years. The events were modeled via a semi-distributed HEC-HMS hydrological model. Land use change was not incorporated in the study because the study only tries to quantify rainfall changes during these two events to simulate the discharge and runoff value. Therefore, the land use data representing the year 2004 were used as inputs in the 2008 runoff model. The study managed to demonstrate that rainfall change has a significant impact to determine the peak discharge and runoff depth for the study area.

DNA profiling analysis of endometrial and ovarian cell lines reveals misidentification, redundancy and contamination.

PubMed

Korch, Christopher; Spillman, Monique A; Jackson, Twila A; Jacobsen, Britta M; Murphy, Susan K; Lessey, Bruce A; Jordan, V Craig; Bradford, Andrew P

2012-10-01

Cell lines derived from human ovarian and endometrial cancers, and their immortalized non-malignant counterparts, are critical tools to investigate and characterize molecular mechanisms underlying gynecologic tumorigenesis, and facilitate development of novel therapeutics. To determine the extent of misidentification, contamination and redundancy, with evident consequences for the validity of research based upon these models, we undertook a systematic analysis and cataloging of endometrial and ovarian cell lines. Profiling of cell lines by analysis of DNA microsatellite short tandem repeats (STR), p53 nucleotide polymorphisms and microsatellite instability was performed. Fifty-one ovarian cancer lines were profiled with ten found to be redundant and five (A2008, OV2008, C13, SK-OV-4 and SK-OV-6) identified as cervical cancer cells. Ten endometrial cell lines were analyzed, with RL-92, HEC-1A, HEC-1B, HEC-50, KLE, and AN3CA all exhibiting unique, uncontaminated STR profiles. Multiple variants of Ishikawa and ECC-1 endometrial cancer cell lines were genotyped and analyzed by sequencing of mutations in the p53 gene. The profile of ECC-1 cells did not match the EnCa-101 tumor, from which it was reportedly derived, and all ECC-1 isolates were genotyped as Ishikawa cells, MCF-7 breast cancer cells, or a combination thereof. Two normal, immortalized endometrial epithelial cell lines, HES cells and the hTERT-EEC line, were identified as HeLa cervical carcinoma and MCF-7 breast cancer cells, respectively. Results demonstrate significant misidentification, duplication, and loss of integrity of endometrial and ovarian cancer cell lines. Authentication by STR DNA profiling is a simple and economical method to verify and validate studies undertaken with these models. Copyright © 2012 Elsevier Inc. All rights reserved.

Spatially resolved air-water emissions tradeoffs improve regulatory impact analyses for electricity generation.

PubMed

Gingerich, Daniel B; Sun, Xiaodi; Behrer, A Patrick; Azevedo, Inês L; Mauter, Meagan S

2017-02-21

Coal-fired power plants (CFPPs) generate air, water, and solids emissions that impose substantial human health, environmental, and climate change (HEC) damages. This work demonstrates the importance of accounting for cross-media emissions tradeoffs, plant and regional emissions factors, and spatially variation in the marginal damages of air emissions when performing regulatory impact analyses for electric power generation. As a case study, we assess the benefits and costs of treating wet flue gas desulfurization (FGD) wastewater at US CFPPs using the two best available treatment technology options specified in the 2015 Effluent Limitation Guidelines (ELGs). We perform a life-cycle inventory of electricity and chemical inputs to FGD wastewater treatment processes and quantify the marginal HEC damages of associated air emissions. We combine these spatially resolved damage estimates with Environmental Protection Agency estimates of water quality benefits, fuel-switching benefits, and regulatory compliance costs. We estimate that the ELGs will impose average net costs of $3.01 per cubic meter for chemical precipitation and biological wastewater treatment and $11.26 per cubic meter for zero-liquid discharge wastewater treatment (expected cost-benefit ratios of 1.8 and 1.7, respectively), with damages concentrated in regions containing a high fraction of coal generation or a large chemical manufacturing industry. Findings of net cost for FGD wastewater treatment are robust to uncertainty in auxiliary power source, location of chemical manufacturing, and binding air emissions limits in noncompliant regions, among other variables. Future regulatory design will minimize compliance costs and HEC tradeoffs by regulating air, water, and solids emissions simultaneously and performing regulatory assessments that account for spatial variation in emissions impacts.

Does des-acyl ghrelin improve glycemic control in obese diabetic subjects by decreasing acylated ghrelin levels?

PubMed

Özcan, Behiye; Neggers, Sebastian J C M M; Miller, Anne Reifel; Yang, Hsiu-Chiung; Lucaites, Virginia; Abribat, Thierry; Allas, Soraya; Huisman, Martin; Visser, Jenny A; Themmen, Axel P N; Sijbrands, Eric J G; Delhanty, Patric J D; van der Lely, Aart Jan

2014-06-01

The objective of this study was to assess the effects of a continuous overnight infusion of des-acyl ghrelin (DAG) on acylated ghrelin (AG) levels and glucose and insulin responses to a standard breakfast meal (SBM) in eight overweight patients with type 2 diabetes. Furthermore, in the same patients and two additional subjects, the effects of DAG infusion on AG concentrations and insulin sensitivity during a hyperinsulinemic-euglycemic clamp (HEC) were assessed. A double-blind, placebo-controlled cross-over study design was implemented, using overnight continuous infusions of 3 and 10  μg DAG/kg per h and placebo to study the effects on a SBM. During a HEC, we studied the insulin sensitivity. We observed that, compared with placebo, overnight DAG administration significantly decreased postprandial glucose levels, both during continuous glucose monitoring and at peak serum glucose levels. The degree of improvement in glycemia was correlated with baseline plasma AG concentrations. Concurrently, DAG infusion significantly decreased fasting and postprandial AG levels. During the HEC, 2.5  h of DAG infusion markedly decreased AG levels, and the M-index, a measure of insulin sensitivity, was significantly improved in the six subjects in whom we were able to attain steady-state euglycemia. DAG administration was not accompanied by many side effects when compared with placebo. DAG administration improves glycemic control in obese subjects with type 2 diabetes through the suppression of AG levels. DAG is a good candidate for the development of compounds in the treatment of metabolic disorders or other conditions with a disturbed AG:DAG ratio, such as type 2 diabetes mellitus or Prader-Willi syndrome. © 2014 European Society of Endocrinology.

[Expression of SLP-2 mRNA in endometrial cancer and its significance].

PubMed

Feng, Wang-qin; Cui, Zhu-mei; Feng, Feng-zhi; Qi, Xiu-juan; Ding, Fang; Li, Wen-dong; Liu, Zhi-hua

2005-08-01

To characterize the differential expression of SLP-2 in endometrial cancer, and to study the effect of human SLP-2 gene on human endometrial cancer cell line. The expression of SLP-2 gene in 32 cases of endometrial cancer and 28 cases of normal endometrial tissues was examined by semi-quantitative RT-PCR. Eukaryotic expression vectors of sense and antisense SLP-2 were constructed and transfected into HEC-1B cell line using lipofectamine 2000 respectively. The morphological changes of the cell were observed by phase contrast microscopy. The cell growth was detected by methyl thiazolyl tetrazolium (MTT) assay, and the cell cycles were analyzed by flow cytometry. The expression of SLP-2 mRNA in endometrial cancer tissues was higher than that in normal endometrial tissues (1.6 +/- 0.7 vs 0.7 +/- 0.3, P < 0.05). Sense and antisense human SLP-2 constructs were transfected into HEC-1B cell line respectively. After being transfected with sense SLP-2, the expression of SLP-2 mRNA in HEC-1B cell line was increased by about 2.4 times that of the control group, the cell growth was accelerated, and the number of cells in G(1) phase was decreased by 12.5%, S phase was increased by 8.0%. After being transfected with antisense SLP-2, the expression of SLP-2 mRNA was declined 50%. The transfected cells showed slower growth, and the number of cells in G(1) phase was significantly increased by 10.5%, and S phase was declined by 9.8%. SLP-2 mRNA shows up-regulation in endometrial cancer tissues, and it may have some relationship with carcinogenesis of endometrial cancer.

Twenty-four hour hyperinsulinemic-euglycemic clamp improves postoperative nitrogen balance only in low insulin sensitivity patients following cardiac surgery.

PubMed

Donatelli, F; Nafi, M; Di Nicola, M; Macchitelli, V; Mirabile, C; Lorini, L; Carli, F

2015-07-01

Critically ill patients often suffer from a protein catabolic state. The aim of this study was to demonstrate that nitrogen balance (NB) in cardiac patients admitted to the intensive care unit (ICU) is related to their insulin sensitivity level and that supraphysiologic doses of insulin can restore anabolism. Twenty-eight patients that were admitted to ICU in enteral and/or parenteral nutrition have been enrolled in this study. All patients received a standard nutrition protocol for at least 3 days before starting the study. These patients received either enteral or parenteral nutrition based on 1.4 kcal/kg/h and 1.1 g/kg/24 h of proteins. Participants were studied for three 24 h periods (P1 , P2 , and P3 ). Twenty-four hour NB was calculated from urinary urea nitrogen excretion, fixed protein and energy intake during each of the three periods (P1 , P2 , and P3 ). Simultaneous to P2, a 24 h hyperinsulinemic-euglycemic clamp (HEC) was performed to determine patients' insulin sensitivity (IS) or insulin resistance (IR), as well as the impact of high doses of insulin on NB. Nitrogen balance remained consistently positive in the IS group regardless of the clamp. In IR patients, NB was negative before the clamp and became positive during P2 and P3 . Insulin sensitivity improved during the HEC in IR patients (P < 0.001). A negative NB was found only in insulin resistant patients admitted to the ICU for more than 7 days. A 24-h period HEC improved NB in these patients. © 2015 The Acta Anaesthesiologica Scandinavica Foundation. Published by John Wiley & Sons Ltd.

Emotion recognition in early Parkinson's disease patients undergoing deep brain stimulation or dopaminergic therapy: a comparison to healthy participants.

PubMed

McIntosh, Lindsey G; Mannava, Sishir; Camalier, Corrie R; Folley, Bradley S; Albritton, Aaron; Konrad, Peter E; Charles, David; Park, Sohee; Neimat, Joseph S

2014-01-01

Parkinson's disease (PD) is traditionally regarded as a neurodegenerative movement disorder, however, nigrostriatal dopaminergic degeneration is also thought to disrupt non-motor loops connecting basal ganglia to areas in frontal cortex involved in cognition and emotion processing. PD patients are impaired on tests of emotion recognition, but it is difficult to disentangle this deficit from the more general cognitive dysfunction that frequently accompanies disease progression. Testing for emotion recognition deficits early in the disease course, prior to cognitive decline, better assesses the sensitivity of these non-motor corticobasal ganglia-thalamocortical loops involved in emotion processing to early degenerative change in basal ganglia circuits. In addition, contrasting this with a group of healthy aging individuals demonstrates changes in emotion processing specific to the degeneration of basal ganglia circuitry in PD. Early PD patients (EPD) were recruited from a randomized clinical trial testing the safety and tolerability of deep brain stimulation (DBS) of the subthalamic nucleus (STN-DBS) in early-staged PD. EPD patients were previously randomized to receive optimal drug therapy only (ODT), or drug therapy plus STN-DBS (ODT + DBS). Matched healthy elderly controls (HEC) and young controls (HYC) also participated in this study. Participants completed two control tasks and three emotion recognition tests that varied in stimulus domain. EPD patients were impaired on all emotion recognition tasks compared to HEC. Neither therapy type (ODT or ODT + DBS) nor therapy state (ON/OFF) altered emotion recognition performance in this study. Finally, HEC were impaired on vocal emotion recognition relative to HYC, suggesting a decline related to healthy aging. This study supports the existence of impaired emotion recognition early in the PD course, implicating an early disruption of fronto-striatal loops mediating emotional function.

Aprepitant, granisetron, and dexamethasone versus palonosetron and dexamethasone for prophylaxis of cisplatin-induced nausea and vomiting in patients with upper gastrointestinal cancer: a randomized crossover phase II trial (KDOG 1002).

PubMed

Ishido, Kenji; Higuchi, Katsuhiko; Azuma, Mizutomo; Sasaki, Tohru; Tanabe, Satoshi; Katada, Chikatoshi; Yano, Takafumi; Wada, Takuya; Koizumi, Wasaburo

2016-10-01

We conducted a randomized trial to compare the safety and effectiveness of aprepitant, granisetron, and dexamethasone (AGD) with those of palonosetron and dexamethasone (PD) in patients who received highly emetogenic chemotherapy (HEC). Patients with esophageal or gastric cancer who were scheduled to receive HEC including at least 60 mg/m of cisplatin as the first-line treatment were randomly assigned to receive AGD (oral aprepitant 125 mg on day 1 and 80 mg on days 2-3; intravenous granisetron 3 mg on day 1; intravenous dexamethasone 6.6 mg on day 1 and oral dexamethasone 4 mg on days 2-3) or PD (intravenous palonosetron 0.75 mg on day 1; intravenous dexamethasone 13.2 mg on day 1 and oral dexamethasone 8 mg on days 2-3). The primary endpoint was a complete response during the overall study period (0-120 h after the start of chemotherapy) in the first cycle. Eighty-five patients were enrolled, and 84 were eligible. The complete response rate did not differ between the treatment groups, but the proportion of patients with no vomiting was significantly higher in the AGD group than in the PD group (81.4 vs. 58.5%; P=0.031). The results of a quality-of-life survey indicated that the proportion of patients with no or minimal impact on daily life in the vomiting domain was significantly higher in the AGD group (79.1 vs. 53.7%; P=0.020). The primary endpoint of complete response was not achieved, but AGD seems to be more effective than PD for the prevention of HEC-induced vomiting.

Investigating the Performance of One- and Two-dimensional Flood Models in a Channelized River Network: A Case Study of the Obion River System

NASA Astrophysics Data System (ADS)

Kalyanapu, A. J.; Dullo, T. T.; Thornton, J. C.; Auld, L. A.

2015-12-01

Obion River, is located in the northwestern Tennessee region, and discharges into the Mississippi River. In the past, the river system was largely channelized for agricultural purposes that resulted in increased erosion, loss of wildlife habitat and downstream flood risks. These impacts are now being slowly reversed mainly due to wetland restoration. The river system is characterized by a large network of "loops" around the main channels that hold water either from excess flows or due to flow diversions. Without data on each individual channel, levee, canal, or pond it is not known where the water flows from or to. In some segments along the river, the natural channel has been altered and rerouted by the farmers for their irrigation purposes. Satellite imagery can aid in identifying these features, but its spatial coverage is temporally sparse. All the alterations that have been done to the watershed make it difficult to develop hydraulic models, which could predict flooding and droughts. This is especially true when building one-dimensional (1D) hydraulic models compared to two-dimensional (2D) models, as the former cannot adequately simulate lateral flows in the floodplain and in complex terrains. The objective of this study therefore is to study the performance of 1D and 2D flood models in this complex river system, evaluate the limitations of 1D models and highlight the advantages of 2D models. The study presents the application of HEC-RAS and HEC-2D models developed by the Hydrologic Engineering Center (HEC), a division of the US Army Corps of Engineers. The broader impacts of this study is the development of best practices for developing flood models in channelized river systems and in agricultural watersheds.

Predicting the occurrence of channelized debris flow by an integrated cascading model: A case study of a small debris flow-prone catchment in Zhejiang Province, China

NASA Astrophysics Data System (ADS)

Wei, Zhen-lei; Xu, Yue-Ping; Sun, Hong-yue; Xie, Wei; Wu, Gang

2018-05-01

Excessive water in a channel is an important factor that triggers channelized debris flows. Floods and debris flows often occur in a cascading manner, and thus, calculating the amount of runoff accurately is important for predicting the occurrence of debris flows. In order to explore the runoff-rainfall relationship, we placed two measuring facilities at the outlet of a small, debris flow-prone headwater catchment to explore the hydrological response of the catchment. The runoff responses generally consisted of a rapid increase in runoff followed by a slower decrease. The peak runoff often occurred after the rainfall ended. The runoff discharge data were simulated by two different modeling approaches, i.e., the NAM model and the Hydrologic Engineering Center-Hydrologic Modeling System (HEC-HMS) model. The results showed that the NAM model performed better than the HEC-HMS model. The NAM model provided acceptable simulations, while the HEC-HMS model did not. Then, we coupled the calculated results of the NAM model with an empirically based debris flow initiation model to obtain a new integrated cascading disaster modeling system to provide improved disaster preparedness and hazard management. In this case study, we found that the coupled model could correctly predict the occurrence of debris flows. Furthermore, we evaluated the effect of the range of input parameter values on the hydrographical shape of the runoff. We also used the grey relational analysis to conduct a sensitivity analysis of the parameters of the model. This study highlighted the important connections between rainfall, hydrological processes, and debris flow, and it provides a useful prototype model system for operational forecasting of debris flows.

Effect of telmisartan on selected adipokines, insulin sensitivity, and substrate utilization during insulin-stimulated conditions in patients with metabolic syndrome and impaired fasting glucose.

PubMed

Wohl, Petr; Krusinová, Eva; Hill, Martin; Kratochvílová, Simona; Zídková, Katerina; Kopecký, Jan; Neskudla, Tomás; Pravenec, Michal; Klementová, Marta; Vrbíková, Jana; Wohl, Pavel; Mlejnek, Petr; Pelikánová, Terezie

2010-10-01

Telmisartan improves glucose and lipid metabolism in rodents. This study evaluated the effect of telmisartan on insulin sensitivity, substrate utilization, selected plasma adipokines and their expressions in subcutaneous adipose tissue (SAT) in metabolic syndrome. Twelve patients with impaired fasting glucose completed the double-blind, randomized, crossover trial. Patients received telmisartan (160 mg/day) or placebo for 3 weeks and vice versa with a 2-week washout period. At the end of each period, a hyperinsulinemic euglycemic clamp (HEC) combined with indirect calorimetry was performed. During HEC (0, 30, and 120 min), plasma levels of adipokines were measured and a needle biopsy (0 and 30 min) of SAT was performed. Fasting plasma glucose was lower after telmisartan compared with placebo (P<0.05). There were no differences in insulin sensitivity and substrate utilization. We found no differences in basal plasma adiponectin, resistin and tumour necrosis factor α (TNFα), but an increase was found in basal leptin, after telmisartan treatment. Insulin-stimulated plasma adiponectin (P<0.05), leptin and resistin (P<0.001) were increased, whereas TNFα was decreased (P<0.05) after telmisartan treatment. Expression of resistin, but not adiponectin, TNFα and leptin was increased after telmisartan treatment. Despite the decrease in fasting plasma glucose, telmisartan does not improve insulin sensitivity and substrate utilization. Telmisartan increases plasma leptin as well as insulin-stimulated plasma adiponectin, leptin and resistin, and decreases plasma TNFα during HEC. Changes in plasma adipokines cannot be explained by their expressions in SAT. The changes in plasma adipokines might be involved in the metabolic effects of telmisartan in metabolic syndrome.

Spatially resolved air-water emissions tradeoffs improve regulatory impact analyses for electricity generation

PubMed Central

Gingerich, Daniel B.; Behrer, A. Patrick; Azevedo, Inês L.

2017-01-01

Coal-fired power plants (CFPPs) generate air, water, and solids emissions that impose substantial human health, environmental, and climate change (HEC) damages. This work demonstrates the importance of accounting for cross-media emissions tradeoffs, plant and regional emissions factors, and spatially variation in the marginal damages of air emissions when performing regulatory impact analyses for electric power generation. As a case study, we assess the benefits and costs of treating wet flue gas desulfurization (FGD) wastewater at US CFPPs using the two best available treatment technology options specified in the 2015 Effluent Limitation Guidelines (ELGs). We perform a life-cycle inventory of electricity and chemical inputs to FGD wastewater treatment processes and quantify the marginal HEC damages of associated air emissions. We combine these spatially resolved damage estimates with Environmental Protection Agency estimates of water quality benefits, fuel-switching benefits, and regulatory compliance costs. We estimate that the ELGs will impose average net costs of $3.01 per cubic meter for chemical precipitation and biological wastewater treatment and $11.26 per cubic meter for zero-liquid discharge wastewater treatment (expected cost-benefit ratios of 1.8 and 1.7, respectively), with damages concentrated in regions containing a high fraction of coal generation or a large chemical manufacturing industry. Findings of net cost for FGD wastewater treatment are robust to uncertainty in auxiliary power source, location of chemical manufacturing, and binding air emissions limits in noncompliant regions, among other variables. Future regulatory design will minimize compliance costs and HEC tradeoffs by regulating air, water, and solids emissions simultaneously and performing regulatory assessments that account for spatial variation in emissions impacts. PMID:28167772

Effects of fasting on insulin action and glucose kinetics in lean and obese men and women.

PubMed

Bergman, Bryan C; Cornier, Marc-Andre; Horton, Tracy J; Bessesen, Daniel H

2007-10-01

The development of insulin resistance in the obese individual could impair the ability to appropriately adjust metabolism to perturbations in energy balance. We investigated a 12- vs. 48-h fast on hepatic glucose production (R(a)), peripheral glucose uptake (R(d)), and skeletal muscle insulin signaling in lean and obese subjects. Healthy lean [n = 14; age = 28.0 +/- 1.4 yr; body mass index (BMI) = 22.8 +/- 0.42] and nondiabetic obese (n = 11; age = 34.6 +/- 2.3 yr; BMI = 36.1 +/- 1.5) subjects were studied following a 12- and 48-h fast during 2 h of rest and a 3-h 40 mUxm(-2)xmin(-1) hyperinsulinemic-euglycemic clamp (HEC). Basal glucose R(a) decreased significantly from the 12- to 48-h fast (lean 1.96 +/- 0.23 to 1.63 +/- 0.15; obese 1.23 +/- 0.07 to 1.07 +/- 0.07 mgxkg(-1)xmin(-1); P = 0.004) and was equally suppressed during the HEC after both fasts. The increase in glucose R(d) during the HEC after the 12-h fast was significantly decreased in lean and obese subjects after the 48-h fast (lean 9.03 +/- 1.17 to 4.16 +/- 0.34, obese 6.10 +/- 0.77 to 3.56 +/- 0.30 mgxkg FFM(-1)xmin(-1); P < 0.001). After the 12- but not the 48-h fast, insulin-stimulated AKT Ser(473) phosphorylation was greater in lean than obese subjects. We conclude that 1) 48 h of fasting produces a marked decline in peripheral insulin action, while suppression of hepatic glucose production is maintained in lean and obese men and women; and 2) the magnitude of this decline is greater in lean vs. obese subjects.

River analysis and floodplain modeling using HEC-GeoRAS/RAS, GIS and ArcGIS: a case study for the Salinas River

NASA Astrophysics Data System (ADS)

Mishra, P. K.; Bernini Campos, H. E.

2016-12-01

The lower portion of the Salinas River in Monterey bay, California has a history of flood, lots of study has been made ab out the water quality since the river provides water for the crops around, but is still in need a detailed study about the river behavior and flood analysis. The floods did significant damage, affecting valuable landing farms, residences and businesses in Monterey County. The first step for this study is comprehend and collect the river bathymetry and surroundings and then analyze the discharge and how it is going to change with time. This thesis develops a model about the specific site, recruiting real data from GIS and performing a flow simulation according to flow data provided by USGS, to verify water surface elevation and floodplain. The ArcMap, developed by ESRI, was used along with an extension (HEC-GeoRAS) because it was indeed the most appropriate model to work with the Digital Elevation Model, develop the floodplain and characterizing the land surface accurately in the study site. The HEC-RAS software, developed by US Army Corp of Engineers, was used to compute one-dimension steady flow and two-dimension unsteady flow, providing flow velocity, water surface elevation and profiles, total surface area, head and friction loss and other characteristics, allowing the analysis of the flow. A mean discharge, a mean peak streamflow and a peak discharge were used for the steady flow and a Hydrograph was used for the unsteady flow, both are based on the 1995 flood and discharge history. This study provides important information about water surface elevation and water flow, allowing stakeholders and the government to analyze solutions to avoid damage to the society and landowners.

Eupatilin, a dietary flavonoid, induces G2/M cell cycle arrest in human endometrial cancer cells.

PubMed

Cho, Jung-Hoon; Lee, Jong-Gyu; Yang, Yeong-In; Kim, Ji-Hyun; Ahn, Ji-Hye; Baek, Nam-In; Lee, Kyung-Tae; Choi, Jung-Hye

2011-08-01

This study is the first to investigate the antiproliferative effect of eupatilin in human endometrial cancer cells. Eupatilin, a naturally occurring flavonoid isolated from Artemisia princeps, has anti-inflammatory, anti-oxidative, and anti-tumor activities. In the present study, we investigated the potential effect of eupatilin on cell growth and its molecular mechanism of action in human endometrial cancer cells. Eupatilin was more potent than cisplatin in inhibiting cell viability in the human endometrial cancer cell lines Hec1A and KLE. Eupatilin showed relatively low cytotoxicity in normal human endometrial cells HES and HESC cells when compared to cisplatin. Eupatilin induced G2/M phase cell cycle arrest in a time- and dose-dependent manner, as indicated by flow cytometry analysis. In addition, treatment of Hec1A cells with eupatilin resulted in a significant increase in the expression of p21(WAF1/CIP1) and in the phosphorylation of Cdc25C and Cdc2. Knockdown of p21 using specific siRNAs significantly compromised eupatilin-induced cell growth inhibition. Interestingly, levels of mutant p53 in Hec1A cells decreased markedly upon treatment with eupatilin, and p53 siRNA significantly increased p21 expression. Moreover, eupatilin modulated the phosphorylation of protein kinases ERK1/2, Akt, ATM, and Chk2. These results suggest that eupatilin inhibits the growth of human endometrial cancer cells via G2/M phase cell cycle arrest through the up-regulation of p21 by the inhibition of mutant p53 and the activation of the ATM/Chk2/Cdc25C/Cdc2 checkpoint pathway. Copyright © 2011 Elsevier Ltd. All rights reserved.

Flood Prediction for the Tam Nong District in Mekong Delta Using Hydrological Modelling and Hydrologic Remote Sensing Technique

NASA Astrophysics Data System (ADS)

Kappas, Martin; Nguyen Hong, Quang; Thanh, Nga Pham Thi; Thu, Hang Le Thi; Nguyen Vu, Giang; Degener, Jan; Rafiei Emam, Ammar

2017-04-01

There has been an increasing attention to the large trans-boundary Mekong river basin due to various problems related to water management and flood control, for instance. Vietnam Mekong delta is located at the downstream of the river basin where is affected most by this human-induced reduction in flows from the upstream. On the other hand, the flood plain of nine anastomosing channels is increasingly effected by the seawater intrusion due to sea level rising of climate change. This results in negative impacts of salinization, drought, and floods, while formerly flooding had frequently brought positive natural gain of irrigation water and alluvial aggradation. In this research, our aim is to predict flooding for the better water management adaptation and control. We applied the model HEC-SSP 2.1 to analyze flood flow frequency, two-dimensional unsteady flow calculations in HEC-RAS 5.0 for simulating a floodplain inundation. Remote sensing-based water level (Jason-2) and inundation map were used for validation and comparison with the model simulations. The results revealed a reduction of water level at all the monitoring stations, particularly in the last decade. In addition, a trend of the inundation extension gradually declined, but in some periods it remained severe due to water release from upstream reservoirs during the rainy season (October-November). We found an acceptable agreement between the HEC-RAS and remote sensing flooding maps (around 70%). Based on the flood routine analysis, we could conclude that the water level will continue lower and lead to a trend of drought and salinization harsher in the near future. Keywords: Mekong delta, flood control, inundation, water management, hydrological modelling, remote sensing

Using HEC-RAS to Enhance Interpretive Capabilities of Geomorphic Assessments

NASA Astrophysics Data System (ADS)

Keefer, L. L.

2005-12-01

The purpose of a geomorphic assessment is to characterize and evaluate a fluvial system for determining the past watershed and channel conditions, current geomorphic character and potential future channel adjustments. The geomorphic assessment approach utilized by the Illinois State Water Survey assesses channel response to disturbance at multiple temporal and spatial scales to help identify the underlying factors and events which led to the existing channel morphology. This is accomplished through two phases of investigation that involve a historical and physical analysis of the watershed, disturbance history, and field work at increasing levels of detail. To infer future channel adjustments, the geomorphic assessment protocol combines two methods of analyses that are dependent on the quantity and detail of the available data. The first method is the compilation of multiple lines of evidence using qualitative information related to the dominant fluvial environment, channel gradient, stream power thresholds, and channel evolution models. The second method is the use of hydraulic models which provide additional interpretative skills to evaluate potential channel adjustments. The structured data collection framework of the geomorphic assessment approach is used for the development of a HEC-RAS model. The model results are then used as another tool to determine the influence of bridges and control structures on channel stability, stream power profiles to identify potential channel bed degradation zones, and provide data for physically-based bank stability models. This poster will demonstrate the advantages of using a hydraulic model, such as HEC-RAS, to expand the interpretive capabilities of geomorphic assessments. The results from applying this approach will be demonstrated for the Big Creek watershed of the Cache River Basin in southern Illinois.

Infrared Spectra of He-, Ne-, and Ar-C_2D_2 Complexes

NASA Astrophysics Data System (ADS)

Rezai, M.; Moazzen-Ahmadi, N.; McKellar, A. R. W.; Fernandez, Berta; Farrelly, David

2012-06-01

Remarkably, there are no previously published experimental spectra of the helium-acetylene van der Waals complex. Apparently, infrared spectra of He-C_2H_2 were recorded around 1990 in Roger Miller's lab, but a detailed rotational assignment was not possible even with the help of two extensive sets of theoretical predictions. Here, we study rare gas-C_2D_2 complexes in the νb{3} region (˜2439 wn) using a rapid-scan tuneable diode laser spectrometer to probe a pulsed supersonic slit-jet expansion. The He-C_2D_2 assignment problem is readily apparent: most of the absorption is piled-up in a very narrow region around 2440.85 wn, close to the R(0) line of the C_2D_2 monomer. This pile-up is a signature of very weak anisotropy in the helium-acetylene intermolecular potential, leading to almost free internal rotation of the C_2D_2. We are able to achieve a convincing rotational assignment with the help of theoretical energy level calculations based on the intermolecular potential surface of Munteanu and Fernández. So far the results are limited to He-C_2D_2 transitions which correlate with the monomer R(0) transition. Ne-C_2D_2 also shows a free-rotation pile-up of lines near R(0) which makes assignment tricky. In contrast, Ar-C_2D_2 exhibits more conventional behavior and a normal asymmetric rotor analysis is possible. [1] T. Slee, R.J. Le Roy, and C.E. Chuaqui, Mol. Phys. 77, 111 (1992); R. Moszynski, P.E.S. Wormer, and A. van der Avoird, J. Chem. Phys. 102, 8385 (1995). [2] R. Munteanu and B. Fernández, J. Chem. Phys. 123, 014309 (2005).

Citizen Science Opportunity With the NASA Heliophysics Education Consortium (HEC)-Radio JOVE Project

NASA Astrophysics Data System (ADS)

Fung, S. F.; Higgins, C.; Thieman, J.; Garcia, L. N.; Young, C. A.

2016-12-01

The Radio JOVE project has long been a hands-on inquiry-based educational project that allows students, teachers and the general public to learn and practice radio astronomy by building their own radio antenna and receiver system from an inexpensive kit that operates at 20.1 MHz and/or using remote radio telescopes through the Internet. Radio JOVE participants observe and analyze natural radio emissions from Jupiter and the Sun. Within the last few years, several Radio JOVE amateurs have upgraded their equipment to make semi-professional spectrographic observations in the frequency band of 15-30 MHz. Due to the widely distributed Radio JOVE observing stations across the US, the Radio JOVE observations can uniquely augment observations by professional telescopes, such as the Long Wavelength Array (LWA) . The Radio JOVE project has recently partnered with the NASA Heliophysics Education Consortium (HEC) to work with students and interested amateur radio astronomers to establish additional spectrograph and single-frequency Radio JOVE stations. These additional Radio JOVE stations will help build a larger amateur radio science network and increase the spatial coverage of long-wavelength radio observations across the US. Our presentation will describe the Radio JOVE project within the context of the HEC. We will discuss the potential for citizen scientists to make and use Radio JOVE observations to study solar radio bursts (particularly during the upcoming solar eclipse in August 2017) and Jovian radio emissions. Radio JOVE observations will also be used to study ionospheric radio scintillation, promoting appreciation and understanding of this important space weather effect.

A study of combined filtration and adsorption on nylon-based dye-affinity membranes: separation of recombinant L-alanine dehydrogenase from crude fermentation broth.

PubMed

Weissenborn, M; Hutter, B; Singh, M; Beeskow, T C; Anspach, F B

1997-04-01

Dextran, hydroxyethylcellulose (HEC), and poly(vinyl alcohol) PVA were covalently linked to bisoxirane-activated nylon membranes. Cibacron Blue F3G-A was immobilized on to these membranes to yield a dye-affinity membrane. The hydrodynamic permeability of affinity membranes was reduced to approximately 50% of that of the original Nylon membrane due to extension of polymer coils into flow-through pores. Adsorption of pre-purified human serum albumin (HSA) and malate dehydrogenase (MDH) displayed highest maximum binding capacities on HEC-coated dye-ligand-affinity membranes, ranging from (163 micrograms/cm2 for HSA to 316 micrograms/cm2 for MDH. The protein recovery of HSA was 100% on dextran-coated membranes compared with 70% on PVA-coated membranes, whereas almost 100% recovery was found for MDH, independent of the polymer. Application of crude supernatant from recombinant Escherichia coli yielded purification factors of 7.4, 8.9 and 11.2 for recombinant alanine dehydrogenase from Mycobacterium tuberculosis for HEC-, dextran- and PVA-coated membranes respectively. Dynamic capacities decreased remarkably to approximately 3 micrograms/cm2 due to co-adsorption of host proteins. The presence of cell debris caused only a slight decrease of purification factors, but a dramatic decrease of the permeability of affinity membranes due to development of a particle layer in front of the membranes. Although enzyme recoveries were up to 90% using cell-free supernatant, more than 50% of the product was lost due to polarization, concentration and rejection at particle layers when using crude homogenates. In order to further improve this integrated downstream process, sophisticated membrane techniques are required by which the formation of a filter cake is circumvented. Further refinement of polymer-coated membranes would not help one to avoid this problem.

Efficacy and safety of olanzapine combined with aprepitant, palonosetron, and dexamethasone for preventing nausea and vomiting induced by cisplatin-based chemotherapy in gynecological cancer: KCOG-G1301 phase II trial.

PubMed

Abe, Masakazu; Hirashima, Yasuyuki; Kasamatsu, Yuka; Kado, Nobuhiro; Komeda, Satomi; Kuji, Shiho; Tanaka, Aki; Takahashi, Nobutaka; Takekuma, Munetaka; Hihara, Hanako; Ichikawa, Yoshikazu; Itonaga, Yui; Hirakawa, Tomoko; Nasu, Kaei; Miyagi, Kanoko; Murakami, Junko; Ito, Kimihiko

2016-02-01

Olanzapine is effective in chemotherapy-induced nausea and vomiting (CINV). In patients receiving highly emetogenic chemotherapy (HEC), its efficacy was reported as rescue therapy for breakthrough emesis refractory to triplet therapy (palonosetron, aprepitant, and dexamethasone). However, its preventive effects with triplet therapy for CINV are unknown. This study aimed to investigate efficacy and safety of preventive use of olanzapine with triplet therapy for CINV of HEC. This study is a prospective multicenter study conducted by Kansai Clinical Oncology Group. Forty chemo-naïve gynecological cancer patients receiving HEC with cisplatin (≥50 mg/m(2)) were enrolled. Oral olanzapine (5 mg) was administered with triplet therapy a day prior to cisplatin administration and on days 1-5. The primary endpoint was complete response (no vomiting and no rescue) rate for the overall phase (0-120 h post-chemotherapy). Secondary endpoints were complete response rate for acute phase (0-24 h post-chemotherapy) and delayed phase (24-120 h post-chemotherapy) and complete control (no vomiting, no rescue, and no significant nausea) rate and total control (no vomiting, no rescue, and no nausea) rate for each phase. These endpoints were evaluated during the first cycle of chemotherapy. Complete response rates for acute, delayed, and overall phases were 97.5, 95.0, and 92.5 %, respectively. Complete control rates were 92.5, 87.5, and 82.5 %, respectively. Total control rates were 87.5, 67.5, and 67.5 %, respectively. There were no grade 3 or 4 adverse events. Preventive use of olanzapine combined with triplet therapy gives better results than those from previously reported studies of triplet therapy.

Supporting the Work Arrangements of Cooperating Teachers and University Supervisors to Better Train Preservice Teachers: A New Theoretical Contribution

ERIC Educational Resources Information Center

Escalié, Guillaume; Chaliès, Sébastien

2016-01-01

This longitudinal case study examines whether a school-based training scheme that brings together different categories of teacher educators (university supervisors and cooperating teachers) engenders true collective training activity and, if so, whether this collective work contributes to pre-service teacher education. The scheme grew out of a…

Hydrologic Modeling in the Kenai River Watershed using Event Based Calibration

NASA Astrophysics Data System (ADS)

Wells, B.; Toniolo, H. A.; Stuefer, S. L.

2015-12-01

Understanding hydrologic changes is key for preparing for possible future scenarios. On the Kenai Peninsula in Alaska the yearly salmon runs provide a valuable stimulus to the economy. It is the focus of a large commercial fishing fleet, but also a prime tourist attraction. Modeling of anadromous waters provides a tool that assists in the prediction of future salmon run size. Beaver Creek, in Kenai, Alaska, is a lowlands stream that has been modeled using the Army Corps of Engineers event based modeling package HEC-HMS. With the use of historic precipitation and discharge data, the model was calibrated to observed discharge values. The hydrologic parameters were measured in the field or calculated, while soil parameters were estimated and adjusted during the calibration. With the calibrated parameter for HEC-HMS, discharge estimates can be used by other researches studying the area and help guide communities and officials to make better-educated decisions regarding the changing hydrology in the area and the tied economic drivers.

Cellulose nanofiber orientation in nanopaper and nanocomposites by cold drawing.

PubMed

Sehaqui, Houssine; Ezekiel Mushi, Ngesa; Morimune, Seira; Salajkova, Michaela; Nishino, Takashi; Berglund, Lars A

2012-02-01

To exploit the mechanical potential of native cellulose fibrils, we report on the preparation of nanopaper with preferred orientation of nanofibrillated cellulose (TEMPO-NFC) by cold drawing. The preparation route is papermaking-like and includes vacuum filtering of a TEMPO-oxidated NFC water dispersion, drawing in wet state and drying. The orientation of the fibrils in the nanopaper was assessed by AFM and wide-angle X-ray diffraction analysis, and the effect on mechanical properties of the resulting nanopaper structure was investigated by tensile tests. At high draw ratio, the degree of orientation is as high as 82 and 89% in-the-plane and cross-sectional planes of the nanopaper, respectively, and the Young's modulus is 33 GPa. This is much higher than mechanical properties of isotropic nanopaper. The cold drawing method can be also applied to NFC nanocomposites as demonstrated by preparation of TEMPO-NFC/hydroxyethyl cellulose (HEC) nanocomposites. The introduction of the soft HEC matrix allows further tailoring of the mechanical properties.

Managing water and riparian habitats on the Bill Williams River with scientific benefit for other desert river systems

USGS Publications Warehouse

John Hickey,; Woodrow Fields,; Andrew Hautzinger,; Steven Sesnie,; Shafroth, Patrick B.; Dick Gilbert,

2016-01-01

This report details modeling to: 1) codify flow-ecology relationships for riparian species of the Bill Williams River as operational guidance for water managers, 2) test the guidance under different climate scenarios, and 3) revise the operational guidance as needed to address the effects of climate change. Model applications detailed herein include the River Analysis System  (HEC-RAS) and the Ecosystem Functions Model  (HEC-EFM), which was used to generate more than three million estimates of local seedling recruitment areas. Areas were aggregated and compared to determine which scenarios generated the most seedling area per unit volume of water. Scenarios that maximized seedling area were grouped into a family of curves that serve as guidance for water managers. This work has direct connections to water management decision-making and builds upon and adds to the rich history of science-based management for the Bill Williams River, Arizona, USA. 

Green synthesis and characterization of hybrid collagen-cellulose-albumin biofibers from skin waste.

PubMed

Amsaveni, Manickam; Anumary, Ayyappan; Ashokkumar, Meiyazhagan; Chandrasekaran, Bangaru; Thanikaivelan, Palanisamy

2013-11-01

Collagen (C) and cellulose are prominent biopolymers from the animal and plant kingdom and widely used in bioengineering. Albumin, on the other hand, is the most abundant plasma protein present in mammalian blood. In this work, collagen extracted from animal skin waste was blended with hydroxyethyl cellulose (HEC) and bovine serum albumin (A) and wet-spun to form hybrid biodegradable C/HEC/A fibers. They were further cross-linked with glutaraldehyde vapors and analyzed. X-ray diffraction and infra-red spectroscopic studies of the hybrid fibers display peaks corresponding to collagen, cellulose, and albumin. Incorporation of cellulose into the biopolymeric matrix leads to a reasonable improvement in mechanical, swelling, and thermal properties of hybrid fibers. Addition of albumin improves the regularity of fiber surface without altering the porosity as observed under a microscope. Hence, the formed hybrid biofibers can be potentially used as a suture material as well as for different biomedical applications due to their improved properties.

Effects of cationic hydroxyethyl cellulose on glucose tolerance and obesity

USDA-ARS?s Scientific Manuscript database

Cholestyramine is a cationic polymer prescribed to lower cholesterol in humans. We investigated the effects of cationic hydroxyethyl cellulose (cHEC) on weight loss and metabolic disorders associated with obesity using both hamster and diet-induced obese mouse models. Golden Syrian hamsters and ob...

Calibrating a hydraulic model using water levels derived from time series high-resolution Radarsat-2 synthetic aperture radar images and elevation data

NASA Astrophysics Data System (ADS)

Trudel, M.; Desrochers, N.; Leconte, R.

2017-12-01

Knowledge of water extent (WE) and level (WL) of rivers is necessary to calibrate and validate hydraulic models and thus to better simulate and forecast floods. Synthetic aperture radar (SAR) has demonstrated its potential for delineating water bodies, as backscattering of water is much lower than that of other natural surfaces. The ability of SAR to obtain information despite cloud cover makes it an interesting tool for temporal monitoring of water bodies. The delineation of WE combined with a high-resolution digital terrain model (DTM) allows extracting WL. However, most research using SAR data to calibrate hydraulic models has been carried out using one or two images. The objectives of this study is to use WL derived from time series high resolution Radarsat-2 SAR images for the calibration of a 1-D hydraulic model (HEC-RAS). Twenty high-resolution (5 m) Radarsat-2 images were acquired over a 40 km reach of the Athabasca River, in northern Alberta, Canada, between 2012 and 2016, covering both low and high flow regimes. A high-resolution (2m) DTM was generated combining information from LIDAR data and bathymetry acquired between 2008 and 2016 by boat surveying. The HEC-RAS model was implemented on the Athabasca River to simulate WL using cross-sections spaced by 100 m. An image histogram thresholding method was applied on each Radarsat-2 image to derive WE. WE were then compared against each cross-section to identify those were the slope of the banks is not too abrupt and therefore amenable to extract WL. 139 observations of WL at different locations along the river reach and with streamflow measurements were used to calibrate the HEC-RAS model. The RMSE between SAR-derived and simulated WL is under 0.35 m. Validation was performed using in situ observations of WL measured in 2008, 2012 and 2016. The RMSE between the simulated water levels calibrated with SAR images and in situ observations is less than 0.20 m. In addition, a critical success index (CSI) was performed to compare the WE simulated by HEC-RAS and that derived from SARs images. The CSI is higher than 0.85 for each date, which means that simulated WE is highly similar to the WE derived from SARs images. Thereby, the results of our analysis indicate that calibration of a hydraulic model can be performed from WL derived from time series of high-resolution SAR images.

Palonosetron versus other 5-HT₃ receptor antagonists for prevention of chemotherapy-induced nausea and vomiting in patients with hematologic malignancies treated with emetogenic chemotherapy in a hospital outpatient setting in the United States.

PubMed

Craver, Chris; Gayle, Julie; Balu, Sanjeev; Buchner, Deborah

2011-01-01

This study evaluated the rate of uncontrolled chemotherapy-induced nausea and vomiting (CINV) after initiating antiemetic prophylaxis with palonosetron versus other 5-HT₃ receptor antagonists (RAs) in patients diagnosed with hematologic malignancies (lymphoma and leukemia) and receiving highly emetogenic chemotherapy (HEC) or moderately emetogenic chemotherapy (MEC) in a hospital outpatient setting. Patients aged ≥ 18 years and diagnosed with hematologic malignancies initiating HEC or MEC and antiemetic prophylaxis with palonosetron (Group 1) and other 5-HT₃ RAs (Group 2) for the first time in a hospital outpatient setting between 4/1/2007 and 3/31/2009 were identified from the Premier Perspective Database. Within each cycle, CINV events were identified (in the hospital outpatient, inpatient, and emergency room settings) through ICD-9 codes for nausea, vomiting, and/or volume depletion (from each CT administration day 1 until the end of the CT cycle), or use of rescue medications (day 2 until the end of the CT cycle). Negative binomial distribution generalized linear multivariate regression model estimating the CINV event rate on CT, specific CT cycles, and cancer diagnosis (leukemia/lymphoma)-matched groups in the follow-up period (first of 8 cycles or 6 months) was developed. Of 971 identified patients, 211 initiated palonosetron (Group 1). Group 1 patients comprised of more females [50.2 vs. 41.4%; p = 0.0226], Whites [74.4 vs. 70.4%, and Hispanics [7.6 vs. 6.3%; all races p = 0.0105], received more HEC treatments [89.6 vs. 84.2%; all CT types p = 0.0129], and had more lymphoma diagnosed patients [89.6 vs. 76.3%; all cancer types p = 0.0033] at baseline. After controlling for differences in several demographic and clinical variables, the regression model predicted a 20.4% decrease in CINV event rate per CT cycle for Group 1 versus Group 2 patients. Study limitations include potential lack of generalizability, absence of data on certain confounders including alcohol consumption and prior history of motion sickness, potential underestimation of incidence of uncontrolled CINV, and inability to draw conclusions pertaining to cause and effect relationship. In this retrospective hospital study, patients with hematologic malignancies treated with HEC or MEC and initiated on antiemetic prophylaxis with palonosetron in the hospital outpatient setting were more likely to experience significantly lower CINV event rates (in the hospital outpatient, inpatient, and emergency room settings) versus patients initiated on other 5-HT₃ RAs.

Rural health prepayment schemes in China: towards a more active role for government.

PubMed

Bloom, G; Shenglan, T

1999-04-01

A large majority of China's rural population were members of health prepayment schemes in the 1970's. Most of these schemes collapsed during the transition to a market economy. Some localities subsequently reestablished schemes. In early 1997 a new government policy identified health prepayment as a major potential source of rural health finance. This paper draws on the experience of existing schemes to explore how government can support implementation of this policy. The decision to support the establishment of health prepayment schemes is part of the government's effort to establish new sources of finance for social services. It believes that individuals are more likely to accept voluntary contributions to a prepayment scheme than tax increases. The voluntary nature of the contributions limits the possibilities for risk-sharing and redistribution between rich and poor. This underlines the need for the government to fund a substantial share of health expenditure out of general revenues, particularly in poor localities. The paper notes that many successful prepayment schemes depend on close supervision by local political leaders. It argues that the national programme will have to translate these measures into a regulatory system which defines the responsibilities of scheme management bodies and local governments. A number of prepayment schemes have collapsed because members did not feel they got value for money. Local health bureaux will have to cooperate with prepayment schemes to ensure that health facilities provide good quality services at a reasonable cost. Users' representatives can also monitor performance. The paper concludes that government needs to clarify the relationship between health prepayment schemes and other actors in rural localities in order to increase the chance that schemes will become a major source rural health finance.

An Investigation of Generic Structures of Pakistani Doctoral Thesis Acknowledgements

ERIC Educational Resources Information Center

Rofess, Sakander; Mahmood, Muhammad Asim

2015-01-01

This paper investigates Pakistani doctoral thesis acknowledgements from genre analysis perspective. A corpus of 235 PhD thesis acknowledgements written in English was taken from Pakistani doctoral theses collected from eight different disciplines. HEC Research Repository of Pakistan was used as a data sources. The theses written by Pakistani…

Mobile Learning: A Kaleidoscope

ERIC Educational Resources Information Center

Kruger, Marlena; Bester, Riana

2014-01-01

CTI is an accredited private higher education institution (university) with the Higher Education Council (HEC) in South Africa. Its head office is in Fourways, Johannesburg. CTI has 12 campuses nationwide and offers higher certificates and degrees in commerce and information technology. These BCom and BSc degrees were rolled out to all 12…

Design and Evaluation of Scour for Bridges Using HEC-18 (Volume 3 of 3).

DOT National Transportation Integrated Search

2017-07-04

The overall objective of this research is the development of a new approach for evaluating bridge scour for New Jersey's bridges on non-tidal waterways. The study commenced with a web-based survey of scour practice within the U.S. and a literature re...

Design and Evaluation of Scour for Bridges Using HEC-18 (Volume 1 of 3).

DOT National Transportation Integrated Search

2017-07-04

The overall objective of this research is the development of a new approach for evaluating bridge scour for New Jersey's bridges on non-tidal waterways. The study commenced with a web-based survey of scour practice within the U.S. and a literature re...

The Impact of HECS Debt on Australian Students' Tertiary Academic Performance

ERIC Educational Resources Information Center

Birch, Elisa R.; Miller, Paul W.

2006-01-01

The Australian literature suggests that students' academic success in tertiary education is principally influenced by their university entrance score. Personal, secondary school and university characteristics have more minor impacts on tertiary outcomes. Little research has been undertaken into the relationship between students' marks and the…

Sediment and Water Quality Insights from the Great Lakes Connecting Channels NCCA Surveys

EPA Science Inventory

Probability-based surveys of the U.S. Great Lakes coastal waters (excluding connecting channels) were conducted in 2010 and 2015 as part of EPA’s National Coastal Condition Assessment (NCCA). Research on the Huron-Erie corridor (HEC; 2014, 2015) and the St. Marys River (SMR; 2015...

Design and Evaluation of Scour for Bridges Using HEC-18 (Volume 2 of 3).

DOT National Transportation Integrated Search

2017-07-04

The overall objective of this research is the development of a new approach for evaluating bridge scour for New Jersey's bridges on non-tidal waterways. The study commenced with a web-based survey of scour practice within the U.S. and a literature re...

Using Student Performance to Judge the Difficulty of Examinations

ERIC Educational Resources Information Center

Roegner, Katherine

2015-01-01

This contribution focuses on a scheme developed to characterize the level of difficulty of an examination in the course "Linear Algebra for Engineers" and on the transfer of the underlying idea to a similar scheme for examinations in the course "Analysis I for Engineers". Using these schemes, it is possible to define standards…

Factors affecting sustainability of rural water schemes in Swaziland

NASA Astrophysics Data System (ADS)

Peter, Graciana; Nkambule, Sizwe E.

The Millennium Development Goal (MDG) target to reduce the proportion of people without sustainable access to safe drinking water by the year 2015 has been met as of 2010, but huge disparities exist. Some regions, particularly Sub-Saharan Africa are lagging behind it is also in this region where up to 30% of the rural schemes are not functional at any given time. There is need for more studies on factors affecting sustainability and necessary measures which when implemented will improve the sustainability of rural water schemes. The main objective of this study was to assess the main factors affecting the sustainability of rural water schemes in Swaziland using a Multi-Criteria Analysis Approach. The main factors considered were: financial, social, technical, environmental and institutional. The study was done in Lubombo region. Fifteen functional water schemes in 11 communities were studied. Data was collected using questionnaires, checklist and focused group discussion guide. A total of 174 heads of households were interviewed. Statistical Package for Social Sciences (SPSS) was used to analyse the data and to calculate sustainability scores for water schemes. SPSS was also used to classify sustainability scores according to sustainability categories: sustainable, partially sustainable and non-sustainable. The averages of the ratings for the different sub-factors studied and the results on the sustainability scores for the sustainable, partially sustainable and non-sustainable schemes were then computed and compared to establish the main factors influencing sustainability of the water schemes. The results indicated technical and social factors as most critical while financial and institutional, although important, played a lesser role. Factors which contributed to the sustainability of water schemes were: functionality; design flow; water fetching time; ability to meet additional demand; use by population; equity; participation in decision making on operation and maintenance; existence of fund for operation and maintenance; willingness to contribute money; existence of a user’s committee; participation in the initial planning and design of the water scheme; and coordination between the local leaders and user’s committee. The main factors which made the schemes unsustainable were: long fetching time; non-involvement in decision making; lack of willingness to contribute funds; absence of users committee; and lack of cooperation between local leaders and the users committee. Water service providers should address the technical, social, financial and institutional factors identified affecting sustainability in their planning and implementation of rural water schemes.

Mapping Mangrove Density from Rapideye Data in Central America

NASA Astrophysics Data System (ADS)

Son, Nguyen-Thanh; Chen, Chi-Farn; Chen, Cheng-Ru

2017-06-01

Mangrove forests provide a wide range of socioeconomic and ecological services for coastal communities. Extensive aquaculture development of mangrove waters in many developing countries has constantly ignored services of mangrove ecosystems, leading to unintended environmental consequences. Monitoring the current status and distribution of mangrove forests is deemed important for evaluating forest management strategies. This study aims to delineate the density distribution of mangrove forests in the Gulf of Fonseca, Central America with Rapideye data using the support vector machines (SVM). The data collected in 2012 for density classification of mangrove forests were processed based on four different band combination schemes: scheme-1 (bands 1-3, 5 excluding the red-edge band 4), scheme-2 (bands 1-5), scheme-3 (bands 1-3, 5 incorporating with the normalized difference vegetation index, NDVI), and scheme-4 (bands 1-3, 5 incorporating with the normalized difference red-edge index, NDRI). We also hypothesized if the obvious contribution of Rapideye red-edge band could improve the classification results. Three main steps of data processing were employed: (1), data pre-processing, (2) image classification, and (3) accuracy assessment to evaluate the contribution of red-edge band in terms of the accuracy of classification results across these four schemes. The classification maps compared with the ground reference data indicated the slightly higher accuracy level observed for schemes 2 and 4. The overall accuracies and Kappa coefficients were 97% and 0.95 for scheme-2 and 96.9% and 0.95 for scheme-4, respectively.

Power Analysis in Flexible Automation

DTIC Science & Technology

1992-12-15

is a motor conlstant, that, rep~resenlts I hec \\-ollllet~ric dlispliacemnent of fluid iii the motor per radian of angula~r inovement .. It is...lIJhA/AI ,.qiup. Theory and Practicc Robots a nd A1an jp ilaiors. Al.Ilsie rdani: Elsevier. pp. 3(66- 374. Zabrow, Poland , 1981. :3. Shin-Min Song and

Urbanization Impacts on Flooding in the Kansas River Basin and Evaluation of Wetlands as a Mitigation Measure

EPA Science Inventory

This study evaluates the impacts of future land use changes on flooding in the Kansas River Basin. It also studies the impacts of wetlands on flood reduction. The study presents Hydrologic Engineering Centers-Hydrologic Modeling System (HEC-HMS) based runoff modeling and River A...

78 FR 57001 - Proposed Collection of Information; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-16

.... Customer Modality Administrative Survey, VA Form 10-0151. b. VA Form Letter 10-491 & HEC Online Survey, 10... processes. The goal of the Customer Modality Survey is to implement a survey process that allows the organization to be proactive in caring for Veterans prior to them receiving services. DATES: Written comments...

Advising Older Homeowners on Home Equity Conversion: A Guide for Attorneys.

ERIC Educational Resources Information Center

Schimeall, Kent M.; Ernst, Trudy A.

This manual is intended to introduce attorneys to the concept of home equity conversion (HEC) so that they can knowledgeably advise elderly homeowners who may be considering this type of transaction. It is directed to attorneys representing the elderly rather than to attorneys representing lenders or investors in home equity conversion…

Teacher Educators' Evaluation of the English Language Teaching Program: A Turkish Case

ERIC Educational Resources Information Center

Yavuz, Aysun; Zehir Topkaya, Ece

2013-01-01

This study explored the perceptions of teacher educators regarding the changes in the English Language Teacher Education Program introduced by the Turkish Higher Education Council (HEC) in 2006. Employing a qualitative design, open-ended questionnaires were administered to 18 lecturers working at five different state universities. The analysis of…

The Special Educator and the Hospital Ethics Committee.

ERIC Educational Resources Information Center

Lowenthal, Barbara; And Others

With the advent of Public Law 99-457, special educators may be expected to observe and/or serve on Hospital Ethics Committees (HECs) in order to better assist families who have seriously ill and handicapped infants and children. Issues considered by such committees include alternative viewpoints on infant euthanasia and their right to life, the…

Leadership, Partnerships, and Organizational Development: Exploring Components of Effectiveness in Three Full-Service Community Schools

ERIC Educational Resources Information Center

Sanders, Mavis

2016-01-01

Full-service community schools are viewed as an approach to improve educational opportunities and outcomes for underserved student populations. The realization of these goals, however, is not guaranteed. According to Richardson's (2009) research-based model of highly effective community schools (HECS), the effectiveness of full-service community…

Creating the Hybrid Electronic Course: An Instructor's Journal.

ERIC Educational Resources Information Center

Ross, Jeff

This paper details the day to day curriculum of an e-mail-based English class at Central Arizona College. The intent of the class--a Hybrid Electronic Course (HEC)--was to expose the students to both independent research and writing, while also giving them opportunities for traditional classroom meetings. An entire semester was compressed into…

75 FR 3540 - Agency Information Collection (Health Eligibility Center (HEC) New Enrollee Survey) Activity...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-21

... used to improve customer service processes for Veterans applying for health care benefits. VA will use this information to determine the quality of customer service given to the Veteran and to identify what... FURTHER INFORMATION CONTACT: Denise McLamb, Enterprise Records Service (005R1B), Department of Veterans...

Evaluation of protease resistance and toxicity of amyloid-like food fibrils from whey, soy, kidney bean, and egg white.

PubMed

Lassé, Moritz; Ulluwishewa, Dulantha; Healy, Jackie; Thompson, Dion; Miller, Antonia; Roy, Nicole; Chitcholtan, Kenny; Gerrard, Juliet A

2016-02-01

The structural properties of amyloid fibrils combined with their highly functional surface chemistry make them an attractive new food ingredient, for example as highly effective gelling agents. However, the toxic role of amyloid fibrils in disease may cause some concern about their food safety because it has not been established unequivocally if consumption of food fibrils poses a health risk to consumers. Here we present a study of amyloid-like fibrils from whey, kidney bean, soy bean, and egg white to partially address this concern. Fibrils showed varied resistance to proteolytic digestion in vitro by either Proteinase K, pepsin or pancreatin. The toxicity of mature fibrils was measured in vitro and compared to native protein, early-stage-fibrillar protein, and sonicated fibrils in two immortalised human cancer cell lines, Caco-2 and Hec-1a. There was no reduction in the viability of either Caco-2 or Hec-1a cells after treatment with a fibril concentration of up to 0.25 mg/mL. Copyright © 2015 Elsevier Ltd. All rights reserved.

Preparation of novel stable antibacterial nanoparticles using hydroxyethylcellulose and application in paper.

PubMed

Wei, Dafu; Chen, Yan; Zhang, Youwei

2016-01-20

Taking advantage of the self-assembly between the components, novel stable antibacterial nanoparticles were efficiently fabricated via a facile one-step co-polymerization of acrylic acid (AA) and N,N'-methylenebisacrylamide (MBA) on a mixed aqueous solution of poly(hexamethylene guanidine hydrochloride) (PHMG) and hydroxyethylcellulose (HEC). The z-average hydrodynamic diameters of the nanoparticles ranged from 220 nm to 450 nm. The inner layer of the nanoparticles is composed of water-insoluble interpolymer complexes of PHMG and PAA networks, while the outer layer is composed of PHMG and HEC. The nanoparticles are stabilized by electrostatic interactions, hydrogen bonding interactions, and the chemical bonds. The nanoparticle solution remained stable in a wide pH range of 2.0-12.0 and at salt concentrations below 0.25 mol/L. The nanoparticles were incorporated into handsheets using a dipping treatment. The resulted handsheets exhibited excellent antimicrobial activities even after multiple water washing treatments. The nanoparticles are promising in fabricating paper, water-based coatings and textiles with permanent antibacterial activity. Copyright © 2015 Elsevier Ltd. All rights reserved.

Low-energy positron scattering upon endohedrals

NASA Astrophysics Data System (ADS)

Amusia, M. Ya.; Chernysheva, L. V.

2017-07-01

We investigate positron scattering upon endohedrals and compare it with electron-endohedral scattering. We show that the polarization of the fullerene shell considerably alters the polarization potential of an atom, stuffed inside a fullerene. This essentially affects both the positron and electron elastic scattering phases as well as corresponding cross sections. Of great importance is also the interaction between the incoming positron and the target electrons that leads to formation of the virtual positronium P˜s. We illustrate the general trend by concrete examples of positron and electron scattering upon endohedrals He@C60 and Ar@C60, and compare it to scattering upon fullerene C60. To obtain the presented results, we have employed new simplified approaches that permit to incorporate the effect of fullerenes polarizability into the He@C60 and Ar@C60 polarization potential and to take into account the virtual positronium formation. Using these approaches, we obtained numeric results that show strong variations in shape and magnitudes of scattering phases and cross sections due to effect of endohedral polarization and P˜s formation.

Occurrence of Putative Virulence Genes in Arcobacter Species Isolated from Humans and Animals

PubMed Central

Douidah, Laid; de Zutter, Lieven; Baré, Julie; De Vos, Paul; Vandamme, Peter; Vandenberg, Olivier; Van den Abeele, Anne-Marie

2012-01-01

Interest in arcobacters in veterinary and human public health has increased since the first report of the isolation of arcobacters from food of animal origin. Since then, studies worldwide have reported the occurrence of arcobacters on food and in food production animals and have highlighted possible transmission, especially of Arcobacter butzleri, to the human population. In humans, arcobacters are associated with enteritis and septicemia. To assess their clinical relevance for humans and animals, evaluation of potential virulence factors is required. However, up to now, little has been known about the mechanisms of pathogenicity. Because of their close phylogenetic affiliation to the food-borne pathogen Campylobacter and their similar clinical manifestations, the presence of nine putative Campylobacter virulence genes (cadF, ciaB, cj1349, hecA, hecB, irgA, mviN, pldA, and tlyA) previously identified in the recent Arcobacter butzleri ATCC 49616 genome sequence was determined in a large set of human and animal Arcobacter butzleri, Arcobacter cryaerophilus, and Arcobacter skirrowii strains after the development of rapid and accurate PCR assays and confirmed by sequencing and dot blot hybridization. PMID:22170914

Independent technical review and analysis of hydraulic modeling and hydrology under low-flow conditions of the Des Plaines River near Riverside, Illinois

USGS Publications Warehouse

Over, Thomas M.; Straub, Timothy D.; Hortness, Jon E.; Murphy, Elizabeth A.

2012-01-01

The U.S. Geological Survey (USGS) has operated a streamgage and published daily flows for the Des Plaines River at Riverside since Oct. 1, 1943. A HEC-RAS model has been developed to estimate the effect of the removal of Hofmann Dam near the gage on low-flow elevations in the reach approximately 3 miles upstream from the dam. The Village of Riverside, the Illinois Department of Natural Resources-Office of Water Resources (IDNR-OWR), and the U. S. Army Corps of Engineers-Chicago District (USACE-Chicago) are interested in verifying the performance of the HEC-RAS model for specific low-flow conditions, and obtaining an estimate of selected daily flow quantiles and other low-flow statistics for a selected period of record that best represents current hydrologic conditions. Because the USGS publishes streamflow records for the Des Plaines River system and provides unbiased analyses of flows and stream hydraulic characteristics, the USGS served as an Independent Technical Reviewer (ITR) for this study.

Effects of oxytetracycline and amoxicillin on development and biomarkers activities of zebrafish (Danio rerio).

PubMed

Oliveira, Rhaul; McDonough, Sakchai; Ladewig, Jessica C L; Soares, Amadeu M V M; Nogueira, António J A; Domingues, Inês

2013-11-01

Antibiotics have been widely used in human and veterinary medicine to treat or prevent diseases. Residues of antibiotics have been found in aquatic environments, but their effects on fish have been not properly investigated. This work aimed to assess the sub-lethal effects of oxytetracycline and amoxicillin on zebrafish development and biomarkers. Embryos and adults were exposed during 96 h to amoxicillin and oxytetracycline following OECD guidelines. Tissues of adults and pools of embryos were used for catalase, glutathione-S-transferases and lactate dehydrogenase determinations. Amoxicillin caused premature hatching (48 h-EC50=132.4 mg/l) whereas oxytetracycline cause delayed hatching of embryos (72 h-EC50=127.6 mg/l). Moreover, both antibiotics inhibited catalase and induced glutathione-S-transferases in zebrafish adults. However, only oxytetracycline induced lactate dehydrogenase. Short-term effects of antibiotics were observed at high doses (mg/l) indicating that physiological impairment in fish populations is unlike to occur. However, effects of chronic exposures to low doses of ABs must be investigated. Copyright © 2013 Elsevier B.V. All rights reserved.

Conservation Reserve Program effects on floodplain land cover management.

PubMed

Jobe, Addison; Kalra, Ajay; Ibendahl, Elise

2018-05-15

Growing populations and industrialized agriculture practices have eradicated much of the United States wetlands along river floodplains. One program available for the restoration of floodplains is the Conservation Reserve Program (CRP). The current research explores the effects CRP land change has on flooding zones, utilizing Flood Modeller and HEC-RAS. Flood Modeller is proven a viable tool for flood modeling within the United States when compared to HEC-RAS. Application of the software is used in the Nodaway River system located in the western halves of Iowa and Missouri to model effects of introducing new forest areas within the region. Flood stage during the conversion first decreases in the early years, before rising to produce greater heights. Flow velocities where CRP land is present are reduced for long-term scopes. Velocity reduction occurs as the Manning's roughness increases due to tree diameter and brush density. Flood zones become more widespread with the implementation of CRP. Future model implementations are recommended to witness the effects of smaller flood recurrence intervals. Copyright © 2018 Elsevier Ltd. All rights reserved.

Assessing the recharge process and importance of montane water to adjacent tectonic valley-plain groundwater using a ternary end-member mixing analysis based on isotopic and chemical tracers

NASA Astrophysics Data System (ADS)

Peng, Tsung-Ren; Zhan, Wen-Jun; Tong, Lun-Tao; Chen, Chi-Tsun; Liu, Tsang-Sen; Lu, Wan-Chung

2018-03-01

A study in eastern Taiwan evaluated the importance of montane water contribution (MC) to adjacent valley-plain groundwater (VPG) in a tectonic suture zone. The evaluation used a ternary natural-tracer-based end-member mixing analysis (EMMA). With this purpose, VPG and three end-member water samples of plain precipitation (PP), mountain-front recharge (MFR), and mountain-block recharge (MBR) were collected and analyzed for stable isotopic compositions (δ 2H and δ 18O) and chemical concentrations (electrical conductivity (EC) and Cl-). After evaluation, Cl- is deemed unsuitable for EMMA in this study, and the contribution fractions of respective end members derived by the δ 18O-EC pair are similar to those derived by the δ 2H-EC pair. EMMA results indicate that the MC, including MFR and MBR, contributes at least 70% (679 × 106 m3 water volume) of the VPG, significantly greater than the approximately 30% of PP contribution, and greater than the 20-50% in equivalent humid regions worldwide. The large MC is attributable to highly fractured strata and the steep topography of studied catchments caused by active tectonism. Furthermore, the contribution fractions derived by EMMA reflect the unique hydrogeological conditions in the respective study sub-regions. A region with a large MBR fraction is indicative of active lateral groundwater flow as a result of highly fractured strata in montane catchments. On the other hand, a region characterized by a large MFR fraction may possess high-permeability stream beds or high stream gradients. Those hydrogeological implications are helpful for water resource management and protection authorities of the studied regions.

Co-Production of Quality in the Applied Education Research Scheme

ERIC Educational Resources Information Center

Ozga, Jenny

2007-01-01

This contribution looks at the ways in which research quality is defined and addressed in the Applied Education Research Scheme (AERS), particularly within the network on Schools and Social Capital, which is one of the four areas of work within the overall AERS scheme. AERS is a five-year programme, funded jointly by the Scottish Executive and the…

Adopt a Care Home: An intergenerational initiative bringing children into care homes.

PubMed

Di Bona, Laura; Kennedy, Sheila; Mountain, Gail

2017-01-01

Dementia friendly communities, in which people living with dementia actively participate and those around them are educated about dementia, may improve the wellbeing of those living with dementia and reduce the associated stigma. The Adopt a Care Home scheme aims to contribute towards this by teaching schoolchildren about dementia and linking them with people living with dementia in a local care home. Forty-one children, 10 people living with dementia and 8 school/care home staff participated in a mixed methods (questionnaires, observations, interviews and focus groups) evaluation to assess the scheme's feasibility and impact. Data were analysed statistically and thematically. The scheme was successfully implemented, increased children's dementia awareness and appeared enjoyable for most participants. Findings, therefore, demonstrate the scheme's potential to contribute towards dementia friendly communities by increasing children's knowledge and understanding of dementia and engaging people living with dementia in an enjoyable activity, increasing their social inclusion.

Validation of different measures of insulin sensitivity of glucose metabolism in dairy cows using the hyperinsulinemic euglycemic clamp test as the gold standard.

PubMed

De Koster, J; Hostens, M; Hermans, K; Van den Broeck, W; Opsomer, G

2016-10-01

The aim of the present research was to compare different measures of insulin sensitivity in dairy cows at the end of the dry period. To do so, 10 clinically healthy dairy cows with a varying body condition score were selected. By performing hyperinsulinemic euglycemic clamp (HEC) tests, we previously demonstrated a negative association between the insulin sensitivity and insulin responsiveness of glucose metabolism and the body condition score of these animals. In the same animals, other measures of insulin sensitivity were determined and the correlation with the HEC test, which is considered as the gold standard, was calculated. Measures derived from the intravenous glucose tolerance test (IVGTT) are based on the disappearance of glucose after an intravenous glucose bolus. Glucose concentrations during the IVGTT were used to calculate the area under the curve of glucose and the clearance rate of glucose. In addition, glucose and insulin data from the IVGTT were fitted in the minimal model to derive the insulin sensitivity parameter, Si. Based on blood samples taken before the start of the IVGTT, basal concentrations of glucose, insulin, NEFA, and β-hydroxybutyrate were determined and used to calculate surrogate indices for insulin sensitivity, such as the homeostasis model of insulin resistance, the quantitative insulin sensitivity check index, the revised quantitative insulin sensitivity check index and the revised quantitative insulin sensitivity check index including β-hydroxybutyrate. Correlation analysis revealed no association between the results obtained by the HEC test and any of the surrogate indices for insulin sensitivity. For the measures derived from the IVGTT, the area under the curve for the first 60 min of the test and the Si derived from the minimal model demonstrated good correlation with the gold standard. Copyright © 2016 Elsevier Inc. All rights reserved.

Mitochondrial lineage sorting in action – historical biogeography of the Hyles euphorbiae complex (Sphingidae, Lepidoptera) in Italy

PubMed Central

2013-01-01

Background Mitochondrial genes are among the most commonly used markers in studies of species’ phylogeography and to draw conclusions about taxonomy. The Hyles euphorbiae complex (HEC) comprises six distinct mitochondrial lineages in the Mediterranean region, of which one exhibits a cryptic disjunct distribution. The predominant mitochondrial lineage in most of Europe, euphorbiae, is also present on Malta; however, it is nowadays strangely absent from Southern Italy and Sicily, where it is replaced by 'italica'. A separate biological entity in Italy is further corroborated by larval colour patterns with a congruent, confined suture zone along the Northern Apennines. By means of historic DNA extracted from museum specimens, we aimed to investigate the evolution of the mitochondrial demographic structure of the HEC in Italy and Malta throughout the Twentieth Century. Results At the beginning of the Twentieth Century, the European mainland lineages were also present at a moderate frequency in Southern Italy and Sicily. The proportion of 'italica' then steadily increased in this area from below 60 percent to near fixation in about 120 years. Thus, geographical sorting of mitochondrial lineages in the HEC was not as complete then as the current demography suggests. The pattern of an integral 'italica' core region and a disjunct euphorbiae distribution evolved very recently. To explain these strong demographic changes, we propose genetic drift due to anthropogenic habitat loss and fragmentation in combination with an impact from recent climate warming that favoured the spreading of the potentially better adapted 'italica' populations. Conclusions The pattern of geographically separated mitochondrial lineages is commonly interpreted as representing long term separated entities. However, our results indicate that such a pattern can emerge surprisingly quickly, even in a widespread and rather common taxon. We thus caution against drawing hasty taxonomic conclusions from biogeographical patterns of mitochondrial markers derived from modern sampling alone. PMID:23594258

Pier and contraction scour prediction in cohesive soils at selected bridges in Illinois

USGS Publications Warehouse

Straub, Timothy D.; Over, Thomas M.

2010-01-01

This report presents the results of testing the Scour Rate In Cohesive Soils-Erosion Function Apparatus (SRICOS-EFA) method for estimating scour depth of cohesive soils at 15 bridges in Illinois. The SRICOS-EFA method for complex pier and contraction scour in cohesive soils has two primary components. The first component includes the calculation of the maximum contraction and pier scour (Zmax). The second component is an integrated approach that considers a time factor, soil properties, and continued interaction between the contraction and pier scour (SRICOS runs). The SRICOS-EFA results were compared to scour prediction results for non-cohesive soils based on Hydraulic Engineering Circular No. 18 (HEC-18). On average, the HEC-18 method predicted higher scour depths than the SRICOS-EFA method. A reduction factor was determined for each HEC-18 result to make it match the maximum of three types of SRICOS run results. The unconfined compressive strength (Qu) for the soil was then matched with the reduction factor and the results were ranked in order of increasing Qu. Reduction factors were then grouped by Qu and applied to each bridge site and soil. These results, and comparison with the SRICOS Zmax calculation, show that less than half of the reduction-factor method values were the lowest estimate of scour; whereas, the Zmax method values were the lowest estimate for over half. A tiered approach to predicting pier and contraction scour was developed. There are four levels to this approach numbered in order of complexity, with the fourth level being a full SRICOS-EFA analysis. Levels 1 and 2 involve the reduction factors and Zmax calculation, and can be completed without EFA data. Level 3 requires some surrogate EFA data. Levels 3 and 4 require streamflow for input into SRICOS. Estimation techniques for both EFA surrogate data and streamflow data were developed.

Developing Sediment Transport and Dredging Prediction Model of Ohio River at Olmsted Locks and Dams Area using HEC-RAS (1D/2D)By Ganesh Raj Ghimire1 and Bruce A. Devantier 2

NASA Astrophysics Data System (ADS)

Ghimire, G. R.

2015-12-01

Sediment deposition is a serious issue in the construction and operation of large reservoir and inland navigation projects in the United States and around the world. Olmsted Locks and Dams in the Ohio River navigation system is facing similar challenges of huge sediment deposition during the ongoing in-wet construction methodology since 1993. HEC-RAS 5.0 integrated with ArcGIS, will be used to yield unsteady 2D hydrodynamic model of Ohio River at Olmsted area. Velocity, suspended sediment, bed sediment and hydrographic survey data acquired from public archives of USGS and USACE Louisville District will be input into the model. Calibration and validation of model will be performed against the measured stage, flow and velocity data. It will be subjected to completely unsteady 1D sediment transport modeling new to HEC-RAS 5.0 which incorporates sediment load and bed gradation via a DSS file, commercial dredging and BSTEM model. Sediment model will be calibrated to replicate the historical bed volume changes. Excavated cross-sections at Olmsted area will also be used to predict the sediment volume trapped inside the ditch over the period between excavations and placement of dam shells at site. Model will attempt to replicate historical dredging volume data and compare with the deposition volume from simulation model to formulate the dredging prediction model. Hence, the results of this research will generate a model that can form a basis for scheduling the dredging event prior to the placement of off-shore cast shells replacing the current as and when required approach of dredging plan. 1 Graduate Student, Department of Civil Engineering, Southern Illinois University Carbondale Carbondale, Illinois, 62901-6603 2 Professor, Department of Civil Engineering, Southern Illinois University Carbondale Carbondale, Illinois, 62901-6603

Divergent migration within lake sturgeon (Acipenser fulvescens) populations: Multiple distinct patterns exist across an unrestricted migration corridor

USGS Publications Warehouse

Kessel, Steven T.; Hondorp, Darryl W.; Holbrook, Christopher; Boase, James C.; Chiotti, Justin A.; Thomas, Michael V.; Wills, Todd C.; Roseman, Edward; Drouin, Richard; Krueger, Charles C.

2018-01-01

Population structure, distribution, abundance, and dispersal arguably underpin the entire field of animal ecology, with consequences for regional species persistence, and provision of ecosystem services. Divergent migration behaviours among individuals or among populations is an important aspect of the ecology of highly-mobile animals, allowing populations to exploit spatially- or temporally-distributed food and space resources.This study investigated the spatial ecology of lake sturgeon (Acipenser fulvescens) within the barrier free Huron-Erie Corridor (HEC), which connects Lake Huron and Lake Erie of the North American Laurentian Great Lakes.Over six years (2011 – 2016), movements of 268 lake sturgeon in the HEC were continuously monitored across the Great Lakes using acoustic telemetry (10 yr battery life acoustic transmitters). Five distinct migration behaviours were identified with hierarchical cluster analysis, based on the phenology and duration of river and lake use.Lake sturgeon in the HEC were found to contain a high level of intraspecific divergent migration, including partial migration with the existence of residents. Specific behaviours included year-round river residency and multiple lake-migrant behaviours that involved movements between lakes and rivers. Over 85% of individuals were assign to migration behaviours as movements were consistently repeated over the study, which suggested migration behaviours were consistent and persistent in lake sturgeon. Differential use of specific rivers or lakes by acoustic-tagged lake sturgeon further subdivided individuals into 14 “contingents” (spatiotemporally segregated subgroups).Contingents associated with one river (Detroit or St. Clair) were rarely detected in the other river, which confirmed that lake sturgeon in the Detroit and St. Clair represent two semi-independent populations that could require separate management consideration for their conservation. The distribution of migration behaviours did not vary between populations, sexes, body size, or among release locations, which indicated that intrapopulation variability in migratory behaviour is a general feature of the spatial ecology of lake sturgeon in un-fragmented landscapes.

Comparison of new generation low-complexity flood inundation mapping tools with a hydrodynamic model

NASA Astrophysics Data System (ADS)

Afshari, Shahab; Tavakoly, Ahmad A.; Rajib, Mohammad Adnan; Zheng, Xing; Follum, Michael L.; Omranian, Ehsan; Fekete, Balázs M.

2018-01-01

The objective of this study is to compare two new generation low-complexity tools, AutoRoute and Height Above the Nearest Drainage (HAND), with a two-dimensional hydrodynamic model (Hydrologic Engineering Center-River Analysis System, HEC-RAS 2D). The assessment was conducted on two hydrologically different and geographically distant test-cases in the United States, including the 16,900 km2 Cedar River (CR) watershed in Iowa and a 62 km2 domain along the Black Warrior River (BWR) in Alabama. For BWR, twelve different configurations were set up for each of the models, including four different terrain setups (e.g. with and without channel bathymetry and a levee), and three flooding conditions representing moderate to extreme hazards at 10-, 100-, and 500-year return periods. For the CR watershed, models were compared with a simplistic terrain setup (without bathymetry and any form of hydraulic controls) and one flooding condition (100-year return period). Input streamflow forcing data representing these hypothetical events were constructed by applying a new fusion approach on National Water Model outputs. Simulated inundation extent and depth from AutoRoute, HAND, and HEC-RAS 2D were compared with one another and with the corresponding FEMA reference estimates. Irrespective of the configurations, the low-complexity models were able to produce inundation extents similar to HEC-RAS 2D, with AutoRoute showing slightly higher accuracy than the HAND model. Among four terrain setups, the one including both levee and channel bathymetry showed lowest fitness score on the spatial agreement of inundation extent, due to the weak physical representation of low-complexity models compared to a hydrodynamic model. For inundation depth, the low-complexity models showed an overestimating tendency, especially in the deeper segments of the channel. Based on such reasonably good prediction skills, low-complexity flood models can be considered as a suitable alternative for fast predictions in large-scale hyper-resolution operational frameworks, without completely overriding hydrodynamic models' efficacy.

Estimation of sensible and latent heat flux from natural sparse vegetation surfaces using surface renewal

NASA Astrophysics Data System (ADS)

Zapata, N.; Martínez-Cob, A.

2001-12-01

This paper reports a study undertaken to evaluate the feasibility of the surface renewal method to accurately estimate long-term evaporation from the playa and margins of an endorreic salty lagoon (Gallocanta lagoon, Spain) under semiarid conditions. High-frequency temperature readings were taken for two time lags ( r) and three measurement heights ( z) in order to get surface renewal sensible heat flux ( HSR) values. These values were compared against eddy covariance sensible heat flux ( HEC) values for a calibration period (25-30 July 2000). Error analysis statistics (index of agreement, IA; root mean square error, RMSE; and systematic mean square error, MSEs) showed that the agreement between HSR and HEC improved as measurement height decreased and time lag increased. Calibration factors α were obtained for all analyzed cases. The best results were obtained for the z=0.9 m ( r=0.75 s) case for which α=1.0 was observed. In this case, uncertainty was about 10% in terms of relative error ( RE). Latent heat flux values were obtained by solving the energy balance equation for both the surface renewal ( LESR) and the eddy covariance ( LEEC) methods, using HSR and HEC, respectively, and measurements of net radiation and soil heat flux. For the calibration period, error analysis statistics for LESR were quite similar to those for HSR, although errors were mostly at random. LESR uncertainty was less than 9%. Calibration factors were applied for a validation data subset (30 July-4 August 2000) for which meteorological conditions were somewhat different (higher temperatures and wind speed and lower solar and net radiation). Error analysis statistics for both HSR and LESR were quite good for all cases showing the goodness of the calibration factors. Nevertheless, the results obtained for the z=0.9 m ( r=0.75 s) case were still the best ones.

Inhibition of Wnt Signaling Pathways Impairs Chlamydia trachomatis Infection in Endometrial Epithelial Cells

PubMed Central

Kintner, Jennifer; Moore, Cheryl G.; Whittimore, Judy D.; Butler, Megan; Hall, Jennifer V.

2017-01-01

Chlamydia trachomatis infections represent the predominant cause of bacterial sexually transmitted infections. As an obligate intracellular bacterium, C. trachomatis is dependent on the host cell for survival, propagation, and transmission. Thus, factors that affect the host cell, including nutrition, cell cycle, and environmental signals, have the potential to impact chlamydial development. Previous studies have demonstrated that activation of Wnt/β-catenin signaling benefits C. trachomatis infections in fallopian tube epithelia. In cervical epithelial cells chlamydiae sequester β-catenin within the inclusion. These data indicate that chlamydiae interact with the Wnt signaling pathway in both the upper and lower female genital tract (FGT). However, hormonal activation of canonical and non-canonical Wnt signaling pathways is an essential component of cyclic remodeling in another prominent area of the FGT, the endometrium. Given this information, we hypothesized that Wnt signaling would impact chlamydial infection in endometrial epithelial cells. To investigate this hypothesis, we analyzed the effect of Wnt inhibition on chlamydial inclusion development and elementary body (EB) production in two endometrial cell lines, Ishikawa (IK) and Hec-1B, in nonpolarized cell culture and in a polarized endometrial epithelial (IK)/stromal (SHT-290) cell co-culture model. Inhibition of Wnt by the small molecule inhibitor (IWP2) significantly decreased inclusion size in IK and IK/SHT-290 cultures (p < 0.005) and chlamydial infectivity (p ≤ 0.01) in both IK and Hec-1B cells. Confocal and electron microscopy analysis of chlamydial inclusions revealed that Wnt inhibition caused chlamydiae to become aberrant in morphology. EB formation was also impaired in IK, Hec-1B and IK/SHT-290 cultures regardless of whether Wnt inhibition occurred throughout, in the middle (24 hpi) or late (36 hpi) during the development cycle. Overall, these data lead us to conclude that Wnt signaling in the endometrium is a key host pathway for the proper development of C. trachomatis. PMID:29322031

Modeling Guru: Knowledge Base for NASA Modelers

NASA Astrophysics Data System (ADS)

Seablom, M. S.; Wojcik, G. S.; van Aartsen, B. H.

2009-05-01

Modeling Guru is an on-line knowledge-sharing resource for anyone involved with or interested in NASA's scientific models or High End Computing (HEC) systems. Developed and maintained by the NASA's Software Integration and Visualization Office (SIVO) and the NASA Center for Computational Sciences (NCCS), Modeling Guru's combined forums and knowledge base for research and collaboration is becoming a repository for the accumulated expertise of NASA's scientific modeling and HEC communities. All NASA modelers and associates are encouraged to participate and provide knowledge about the models and systems so that other users may benefit from their experience. Modeling Guru is divided into a hierarchy of communities, each with its own set forums and knowledge base documents. Current modeling communities include those for space science, land and atmospheric dynamics, atmospheric chemistry, and oceanography. In addition, there are communities focused on NCCS systems, HEC tools and libraries, and programming and scripting languages. Anyone may view most of the content on Modeling Guru (available at http://modelingguru.nasa.gov/), but you must log in to post messages and subscribe to community postings. The site offers a full range of "Web 2.0" features, including discussion forums, "wiki" document generation, document uploading, RSS feeds, search tools, blogs, email notification, and "breadcrumb" links. A discussion (a.k.a. forum "thread") is used to post comments, solicit feedback, or ask questions. If marked as a question, SIVO will monitor the thread, and normally respond within a day. Discussions can include embedded images, tables, and formatting through the use of the Rich Text Editor. Also, the user can add "Tags" to their thread to facilitate later searches. The "knowledge base" is comprised of documents that are used to capture and share expertise with others. The default "wiki" document lets users edit within the browser so others can easily collaborate on the same document, even allowing the author to select those who may edit and approve the document. To maintain knowledge integrity, all documents are moderated before they are visible to the public. Modeling Guru, running on Clearspace by Jive Software, has been an active resource to the NASA modeling and HEC communities for more than a year and currently has more than 100 active users. SIVO will soon install live instant messaging support, as well as a user-customizable homepage with social-networking features. In addition, SIVO plans to implement a large dataset/file storage capability so that users can quickly and easily exchange datasets and files with one another. Continued active community participation combined with periodic software updates and improved features will ensure that Modeling Guru remains a vibrant, effective, easy-to-use tool for the NASA scientific community.

The Impact of Corps Flood Control Reservoirs in the June 2008 Upper Mississippi Flood

NASA Astrophysics Data System (ADS)

Charley, W. J.; Stiman, J. A.

2008-12-01

The US Army Corps of Engineers is responsible for a multitude of flood control project on the Mississippi River and its tributaries, including levees that protect land from flooding, and dams to help regulate river flows. The first six months of 2008 were the wettest on record in the upper Mississippi Basin. During the first 2 weeks of June, rainfall over the Midwest ranged from 6 to as much as 16 inches, overwhelming the flood protection system, causing massive flooding and damage. Most severely impacted were the States of Iowa, Illinois, Indiana, Missouri, and Wisconsin. In Iowa, flooding occurred on almost every river in the state. On the Iowa River, record flooding occurred from Marshalltown, Iowa, downstream to its confluence with the Mississippi River. At several locations, flooding exceeded the 500-year event. The flooding affected agriculture, transportation, and infrastructure, including homes, businesses, levees, and other water-control structures. It has been estimated that there was at least 7 billion dollars in damages. While the flooding in Iowa was extraordinary, Corps of Engineers flood control reservoirs helped limit damage and prevent loss of life, even though some reservoirs were filled beyond their design capacity. Coralville Reservoir on the Iowa River, for example, filled to 135% of its design flood storage capacity, with stage a record five feet over the crest of the spillway. In spite of this, the maximum reservoir release was limited to 39,500 cfs, while a peak inflow of 57,000 cfs was observed. CWMS, the Corps Water Management System, is used to help regulate Corps reservoirs, as well as track and evaluate flooding and flooding potential. CWMS is a comprehensive data acquisition and hydrologic modeling system for short-term decision support of water control operations in real time. It encompasses data collection, validation and transformation, data storage, visualization, real time model simulation for decision-making support, and data dissemination. The system uses precipitation and flow data, collected in real-time, along with forecasted flow from the National Weather Service to model and optimize reservoir operations and forecast downstream flows and stages, providing communities accurate and timely information to aid their flood-fighting. This involves integrating several simulation modeling programs, including HEC-HMS to forecast flows, HEC-ResSim to model reservoir operations and HEC-RAS to compute forecasted stage hydrographs. An inundation boundary and depth map of water in the flood plain can be calculated from the HEC-RAS results using ArcInfo. By varying future precipitation and releases, engineers can evaluate different "What if?" scenarios. The effectiveness of this tool and Corps reservoirs are examined.

Numerical Study on the Partitioning of the Molecular Polarizability into Fluctuating Charge and Induced Atomic Dipole Contributions

PubMed Central

Mei, Ye; Simmonett, Andrew C.; Pickard, Frank C.; DiStasio, Robert A.; Brooks, Bernard R.; Shao, Yihan

2015-01-01

In order to carry out a detailed analysis of the molecular static polarizability, which is the response of the molecule to a uniform external electric field, the molecular polarizability was computed using the finite-difference method for 21 small molecules, using density functional theory. Within nine charge population schemes (Löwdin, Mulliken, Becke, Hirshfeld, CM5, Hirshfeld-I, NPA, CHELPG, MK-ESP) in common use, the charge fluctuation contribution is found to dominate the molecular polarizability, with its ratio ranging from 59.9% with the Hirshfeld or CM5 scheme to 96.2% with the Mulliken scheme. The Hirshfeld-I scheme is also used to compute the other contribution to the molecular polarizability coming from the induced atomic dipoles, and the atomic polarizabilities in 8 small molecules and water pentamer are found to be highly anisotropic for most atoms. Overall, the results suggest that (a) more emphasis probably should be placed on the charge fluctuation terms in future polarizable force field development; (b) an anisotropic polarizability might be more suitable than an isotropic one in polarizable force fields based entirely or partially on the induced atomic dipoles. PMID:25945749

Numerical study on the partitioning of the molecular polarizability into fluctuating charge and induced atomic dipole contributions

DOE PAGES

Mei, Ye; Simmonett, Andrew C.; Pickard, IV, Frank C.; ...

2015-05-06

In order to carry out a detailed analysis of the molecular static polarizability, which is the response of the molecule to a uniform external electric field, the molecular polarizability was computed in this study using the finite-difference method for 21 small molecules, using density functional theory. Within nine charge population schemes (Lowdin, Mulliken, Becke, Hirshfeld, CM5, Hirshfeld-I, NPA, CHELPG, MK-ESP) in common use, the charge fluctuation contribution is found to dominate the molecular polarizability, with its ratio ranging from 59.9% with the Hirshfeld or CM5 scheme to 96.2% with the Mulliken scheme. The Hirshfeld-I scheme is also used to computemore » the other contribution to the molecular polarizability coming from the induced atomic dipoles, and the atomic polarizabilities in eight small molecules and water pentamer are found to be highly anisotropic for most atoms. In conclusion, the overall results suggest that (a) more emphasis probably should be placed on the charge fluctuation terms in future polarizable force field development and (b) an anisotropic polarizability might be more suitable than an isotropic one in polarizable force fields based entirely or partially on the induced atomic dipoles.« less

Floodplain Modeling in the Kansas River Basin Using Hydrologic Engineering Center (HEC) Models: Impacts of Urbanization and Wetlands for Mitigation

EPA Science Inventory

Flooding is a major natural hazard which every year impacts different regions across the world. Between 2000 and 2008, various types of natural hazards, mainly floods have affected the largest number of people worldwide, averaging 99 million people per year (WDR, 2010). In the U...

Morphological and electrical properties of SrTiO3/TiO2/SrTiO3 sandwich structures prepared by plasma sputtering

NASA Astrophysics Data System (ADS)

Jabbar, Saqib; Ahmad, Riaz; Chu, Paul K.

2017-01-01

Not Available Project supported by the City University of Hong Kong Applied Research Grant (ARG) of China (Grant No. 9667122). One of the authors, Mr. Saqib Jabbar, is grateful to the Higher Education Commission (HEC) of Pakistan for financial support under IRSIP.

The Implementation and Evaluation of a Clinical Supervision Model in Teacher Education in Turkey: Is It an Effective Method?

ERIC Educational Resources Information Center

Bulunz, Nermin; Gursoy, Esim; Kesner, John; Baltaci Goktalay, Sehnaz; Salihoglu, Umut M.

2014-01-01

Implementation of the standards established by the Higher Education Council (HEC) has shown great variation between universities, between departments and even between supervisors. A TUBITAK (111K162)-EVRENA project designed to develop a "teaching practice program" using a Clinical Supervision Model (CSM) was conducted. The present study…

A physically-based channel-modeling framework integrating HEC-RAS sediment transport capabilities and the USDA-ARS bank-stability and toe-erosion model (BSTEM)

USDA-ARS?s Scientific Manuscript database

Classical, one-dimensional, mobile bed, sediment-transport models simulate vertical channel adjustment, raising or lowering cross-section node elevations to simulate erosion or deposition. This approach does not account for bank erosion processes including toe scour and mass failure. In many systems...

A remark on the GNSS single difference model with common clock scheme for attitude determination

NASA Astrophysics Data System (ADS)

Chen, Wantong

2016-09-01

GNSS-based attitude determination technique is an important field of study, in which two schemes can be used to construct the actual system: the common clock scheme and the non-common clock scheme. Compared with the non-common clock scheme, the common clock scheme can strongly improve both the reliability and the accuracy. However, in order to gain these advantages, specific care must be taken in the implementation. The cares are thus discussed, based on the generating technique of carrier phase measurement in GNSS receivers. A qualitative assessment of potential phase bias contributes is also carried out. Possible technical difficulties are pointed out for the development of single-board multi-antenna GNSS attitude systems with a common clock.

The effects of nutrition labeling on consumer food choice: a psychological experiment and computational model.

PubMed

Helfer, Peter; Shultz, Thomas R

2014-12-01

The widespread availability of calorie-dense food is believed to be a contributing cause of an epidemic of obesity and associated diseases throughout the world. One possible countermeasure is to empower consumers to make healthier food choices with useful nutrition labeling. An important part of this endeavor is to determine the usability of existing and proposed labeling schemes. Here, we report an experiment on how four different labeling schemes affect the speed and nutritional value of food choices. We then apply decision field theory, a leading computational model of human decision making, to simulate the experimental results. The psychology experiment shows that quantitative, single-attribute labeling schemes have greater usability than multiattribute and binary ones, and that they remain effective under moderate time pressure. The computational model simulates these psychological results and provides explanatory insights into them. This work shows how experimental psychology and computational modeling can contribute to the evaluation and improvement of nutrition-labeling schemes. © 2014 New York Academy of Sciences.

Excellence in Business Education (A "FRUCE" Model for Higher Education Commission-Recognized Business Schools in Pakistan)

ERIC Educational Resources Information Center

Kolachi, Nadir Ali; Mohammad, Jan

2013-01-01

The paper develops a new model of the essential factors required to be a top business school in the world for the benefit of schools recognized by the Higher Education Commission (HEC) in Pakistan. Globally, top business schools are those that excel in research, attract strong faculty, and successfully foster student development. The present…

Hunter Ethics: A Look at Hunter Behavior and Hunter Education in the U.S. and Canada.

ERIC Educational Resources Information Center

Elliott, Denis S.

This study examines issues of unethical hunting practices and promotion of ethical hunter behavior in the United States and Canada. Hunter education coordinators (HECs) in all states and provinces were surveyed, with a response rate of 66 percent. Results indicate: (1) there has been little change in the types of hunter ethics problems in recent…

Federal microcomputer software for urban hydrology

USGS Publications Warehouse

Jennings, Marshall E.; Smith, Roger H.; Jennings, Ross B.

1988-01-01

The purpose of this paper is to describe the development, availability, and general use of selected urban hydrology microcomputer software developed by: U.S. Soil Conservation Service (SCS); U.S. Army Corps of Engineers, Hydrologic Engineering Center (HEC); U.S. Environmental Protection Agency (EPA); and U.S. Geological Survey (USGS). The discussion is limited to software used for design and planning for urban stormwater flows.

Sleep and Alertness Management III: Effects of a Nap and Hypnotics on Performance During the Late Evening, Night and Early Morning in Marmosets

DTIC Science & Technology

2006-11-01

positive reinforcement as a motivating stimulus (small pieces of marshmallow ) has been used to asses the HEC [Philippens et al. 2000]. The marmoset...apparatus using positive reinforcement as a motivating stimulus for the animals (small pieces of marshmallow ) has been used to asses the Hand-eye

Interactions between sodium dodecyl sulphate and non-ionic cellulose derivatives studied by size exclusion chromatography with online multi-angle light scattering and refractometric detection.

PubMed

Wittgren, Bengt; Stefansson, Morgan; Porsch, Bedrich

2005-08-05

The novel approach described allows to characterise the surfactant-polymer interaction under several sodium dodecyl sulphate (SDS) concentrations (0-20 mM) using size exclusion chromatography (SEC) with online multi-angle light scattering (MALS) and refractometric (RI) detection. Three different cellulose derivatives, hydroxypropyl cellulose (HPC), hydroxypropyl methyl cellulose (HPMC) and hydroxyethyl cellulose (HEC), have been studied in solution containing 10 mM NaCl and various concentrations of sodium dodecyl sulphate. It is shown that this approach is well suited for successful application of both Hummel-Dreyer and multi-component light scattering principles and yields reliable molecular masses of both the polymer complex and the polymer itself within the complex, the amount of surfactant bound into the complex as well as appropriate values of the refractive index increment (dn/dc)micro, of both the complex and the polymer in question. The more hydrophobic derivatives HPC and HPMC adsorbed significantly more SDS than HEC. The inter-chain interactions close to critical aggregation concentration (cac) were clearly seen for HPC and HPMC as an almost two-fold average increase in polymer molecular mass contained in the complex.

Tyrosol and its metabolites as antioxidative and anti-inflammatory molecules in human endothelial cells.

PubMed

Muriana, Francisco J G; Montserrat-de la Paz, Sergio; Lucas, Ricardo; Bermudez, Beatriz; Jaramillo, Sara; Morales, Juan C; Abia, Rocio; Lopez, Sergio

2017-08-01

Tyrosol (Tyr) is a phenolic compound found in virgin olive oil. After ingestion, Tyr undergoes extensive first pass intestinal/hepatic metabolism. However, knowledge about the biological effects of Tyr metabolites is scarce. We chemically synthesized Tyr glucuronate (Tyr-GLU) and sulphate (Tyr-SUL) metabolites and explored their properties against oxidative stress and inflammation in TNF-α-treated human umbilical vein endothelial cells (hECs). Tyr and Tyr-SUL prevented the rise of reactive oxygen species, the depletion of glutathione, and the down-regulation of glutathione peroxidase 1, glutamate-cysteine ligase catalytic subunit, and heme oxygenase-1 genes. Tyr-SUL and to a lower extent Tyr and Tyr-GLU prevented the phosphorylation of NF-κB signaling proteins. Tyr-GLU and Tyr-SUL also prevented the over-expression of adhesion molecules at gene, protein, and secretory levels, and the adhesion (Tyr-SUL > Tyr-GLU) of human monocytes to hECs. In vivo, Tyr, and most notably Tyr-SUL in a dose-dependent manner, ameliorated plantar and ear edemas in mice models of acute and chronic inflammation. This study demonstrates the antioxidant and/or anti-inflammatory properties of Tyr metabolites, with Tyr-SUL being the most effective.

Determination of Heavy Metal Concentrations in Normal and Pathological Human Endometrial Biopsies and In Vitro Regulation of Gene Expression by Metals in the Ishikawa and Hec-1b Endometrial Cell Line

PubMed Central

Tomkiewicz, Céline; Leblanc, Alix; Pierre, Stéphane; El Balkhi, Souleiman; Le Frère-Belda, Marie-Aude; Lecuru, Fabrice; Poupon, Joël; Barouki, Robert; Aggerbeck, Martine; Coumoul, Xavier

2015-01-01

It is well known that several metals, such as lead, mercury, cadmium, and vanadium, can mimic the effects of estrogens (metallo-estrogens). Nevertheless, there are only a few studies that have assessed the effects of toxic metals on the female genital tract and, in particular, endometrial tissue. In this context, we measured the concentrations of several trace elements in human endometrial tissue samples from individuals with hyperplasia or adenocarcinoma and in normal tissues. Hyperplasic endometrial tissue has a 4-fold higher concentration of mercury than normal tissue. Mercury can affect both the AhR and ROS signaling pathways. Thus, we investigated the possible toxic effects of mercury by in vitro studies. We found that mercury increases oxidative stress (increased HO1 and NQO1 mRNA levels) and alters the cytoskeleton in the human endometrial Ishikawa cell line and to a lesser extent, in the “less-differentiated” human endometrial Hec-1b cells. The results might help to explain a potential link between this metal and the occurrence of endometrial hyperplasia. PMID:26600472

Deoxycholate-hydrogels: novel drug carrier systems for topical use.

PubMed

Valenta, C; Nowack, E; Bernkop-Schnürch, A

1999-08-05

Na-deoxycholate (Na-DOC) forms a viscous thixotropic gel when in contact with excess buffer systems. The resulting gels have been tested as novel drug carrier systems for topical use. The influence of differing amounts of mannitol, glycerol and xylitol on the viscous modulus (G"/Pa) was evaluated by oscillatory measurements. Na-DOC (0.5%) in phosphate buffered saline (PBS) with 5% mannitol was chosen as an optimised formulation, taking into account viscosity, distribution and appearance. The release rate of the model drug rutin through an artificial membrane was higher than those from hydroxyethylcellulose- (HEC) and sodium polyacrylate (NaC934)-gels; permeation through excised rat skin was also highest for the Na-DOC systems. The results indicate that Na-DOC significantly increases the membrane permeability. The microbial stability was in the same range as HEC- and NaC934-gels, making a preservation necessary. Na-DOC-gels are novel low molecular weight, multifunctional drug carriers, which also act as penetration enhancers. Their thixotropy is an additional advantage for better application to large skin areas, nasal, vaginal and buccal membranes. Therefore, Na-DOC-gels can be considered promising, alternative drug carrier systems for topical pharmaceutical as well as cosmetic use.

"Flooding Risk Analysis and the Understanding of Hydrological Disturbance due to the Rapid Urbanization in a Low-Scale Subwatershed in Houston Area". ( The project develops a relavant Model of flooding risk assessment to define the connection between increased streamflow/flooding and the rapid urban land development).

NASA Astrophysics Data System (ADS)

Geldiyev, P.

2017-12-01

Rapid urban development and changing climate influences the frequency and magnitude of flooding in Houston area. This proposed project aims to evaluate the flooding risks with the current and future land use changes by 2040 for one subbasin of the San Jacinto Brazos/Neches-Trinity Coastal basin. Surface environments and streamflow data of the Clear Creek are analyzed and stimulated to discuss the possible impact of urbanization on the occurrence of floods. The streamflow data is analyzed and simulated with the application of the Geographic Information Systems and its extensions. Both hydrologic and hydraulic models of the Clear Creek are created with the use of HEC-HMS and HEC-RAS software. Both models are duplicated for the year 2040, based on projected 2040 Landcover Maps developed by Houston and Galveston Area Council. This project examines a type of contemporary hydrologic disturbance and the interaction between land cover and changes in hydrological processes. Expected results will be very significant for urban development and flooding management.

Analysis of flood vulnerability in urban area; a case study in deli watershed

NASA Astrophysics Data System (ADS)

Indrawan, I.; Siregar, R. I.

2018-03-01

Based on the National Disaster Management Agency of Indonesia, the distribution of disasters and victims died until the year 2016 is the largest flood disaster. Deli River is a river that has the greatest flood potential through Medan City. In Deli Watershed, flow discharge affected by the discharge from its tributaries, the high rainfall intensity and human activity. We should anticipate reducing and preventing the occurrence of losses due to flood damage. One of the ways to anticipate flood disaster is to predict which part of urban area is would flood. The objective of this study is to analyze the flood inundation areas due to overflow of Deli River through Medan city. Two-dimensional modeling by HEC-RAS 5.0.3 is a widely used hydraulic software tool developed by the U.S Army Corps of Engineers, which combined with the HEC-HMS for hydrological modeling. The result shows flood vulnerability in Medan by a map to present the spot that vulnerable about flood. The flooded area due to the overflowing of Deli River consists of seven sub districts, namely Medan Johor, Medan Selayang, Medan Kota, Medan Petisah, Medan Maimun, Medan Perjuangan and Medan Barat.

Potentiality of rainwater harvesting for an urban community in Bangladesh

NASA Astrophysics Data System (ADS)

Akter, Aysha; Ahmed, Shoukat

2015-09-01

Due to cost effectiveness, rainwater harvesting (RWH) systems are practicing already in some rural parts of Bangladesh but very few in urban areas. This paper aimed to evaluate the potentiality of RWH systems in the South Agrabad in Chittagong city with an average annual precipitation of 3000 mm, experiencing both water scarcity and urban flooding in the same year. The adopted approach was Analytic Hierarchy Process (AHP) based multicriteria decision analysis technique, and the evaluation criteria were roof area, slope, drainage density and runoff coefficient. Geospatial Hydrologic Modeling Extension supported hydrologic model viz. HEC-HMS used to simulate the precipitation-runoff process, the model outcomes showed RWH potentiality which could minimize stagnant storm water up to 26% through supplementing city water supply annually up to 20 liter/person/day. Then, assigning suitable weightage to the evaluation criteria with their associated features in ArcGIS 9.3, the study area was reasonably divided into three potential zones i.e. good, moderate and poor covering 19%, 64% and 17% of the total area respectively. Thus, this is envisaged AHP using HEC-HMS could provide important guidance to the decision supporting system not only for urban areas but also for the wide sub-basin/basin context.

Design of a shape-memory alloy actuated macro-scale morphing aircraft mechanism

NASA Astrophysics Data System (ADS)

Manzo, Justin; Garcia, Ephrahim; Wickenheiser, Adam; Horner, Garnett C.

2005-05-01

As more alternative, lightweight actuators have become available, the conventional fixed-wing configuration seen on modern aircraft is under investigation for efficiency on a broad scale. If an aircraft could be designed with multiple functional equilibria of drastically varying aerodynamic parameters, one craft capable of 'morphing' its shape could be used to replace two or three designed with particular intentions. One proposed shape for large-scale (geometry change on the same order of magnitude as wingspan) morphing is the Hyper-Elliptical Cambered Span (HECS) wing, designed at NASA Langley to be implemented on an unmanned aerial vehicle (UAV). Proposed mechanisms to accomplish the spanwise curvature (in the y-z plane of the craft) that allow near-continuous bending of the wing are narrowed to a tendon-based DC motor actuated system, and a shape memory alloy-based (SMA) mechanism. At Cornell, simulations and wind tunnel experiments assess the validity of the HECS wing as a potential shape for a blended-wing body craft with the potential to effectively serve the needs of two conventional UAVs, and analyze the energetics of actuation associated with a morphing maneuver accomplished with both a DC motor and SMA wire.

Simulation of Flow, Sediment Transport, and Sediment Mobility of the Lower Coeur d'Alene River, Idaho

USGS Publications Warehouse

Berenbrock, Charles; Tranmer, Andrew W.

2008-01-01

A one-dimensional sediment-transport model and a multi-dimensional hydraulic and bed shear stress model were developed to investigate the hydraulic, sediment transport, and sediment mobility characteristics of the lower Coeur d?Alene River in northern Idaho. This report documents the development and calibration of those models, as well as the results of model simulations. The one-dimensional sediment-transport model (HEC-6) was developed, calibrated, and used to simulate flow hydraulics and erosion, deposition, and transport of sediment in the lower Coeur d?Alene River. The HEC-6 modeled reach, comprised of 234 cross sections, extends from Enaville, Idaho, on the North Fork of the Coeur d?Alene River and near Pinehurst, Idaho, on the South Fork of the river to near Harrison, Idaho, on the main stem of the river. Bed-sediment samples collected by previous investigators and samples collected for this study in 2005 were used in the model. Sediment discharge curves from a previous study were updated using suspended-sediment samples collected at three sites since April 2000. The HEC-6 was calibrated using river discharge and water-surface elevations measured at five U.S. Geological Survey gaging stations. The calibrated HEC-6 model allowed simulation of management alternatives to assess erosion and deposition from proposed dredging of contaminated streambed sediments in the Dudley reach. Four management alternatives were simulated with HEC-6. Before the start of simulation for these alternatives, seven cross sections in the reach near Dudley, Idaho, were deepened 20 feet?removing about 296,000 cubic yards of sediments?to simulate dredging. Management alternative 1 simulated stage-discharge conditions from 2000, and alternative 2 simulated conditions from 1997. Results from alternatives 1 and 2 indicated that about 6,500 and 12,300 cubic yards, respectively, were deposited in the dredged reach. These figures represent 2 and 4 percent, respectively, of the total volume of dredged sediments removed before the start of simulation. In alternatives 3 and 4, the incoming total sediment discharges from the South Fork of the river were decreased by one-half. Management alternative 3 simulated stage-discharge conditions from 2000, and alternative 4 simulated conditions from 1997. Reducing incoming sediment discharge from the South Fork did not affect the streambed and deposition in the Dudley and downstream reaches, probably because the distance between the South Fork and the Dudley reach is long enough for sediment supply, transport capacity, and channel geometry to be balanced before reaching the Dudley and downstream reaches. Development and calibration of a multi-dimensional hydraulic and bed shear stress model (FASTMECH) allowed simulation of water-surface elevation, depth, velocity, bed shear stress, and sediment mobility in the Dudley reach (5.3 miles). The computational grid incorporated bathymetric and Light Detection and Ranging (LIDAR) data, with a node spacing of about 2.5 meters. With the exception of the fourth FASTMECH calibration simulation, results from the FASTMECH calibration simulations indicated that flow depths, flow velocities, and bed shear stresses increased as river discharge increased. Water-surface elevations in the fourth calibration simulation were about 2 feet higher than those in the other simulations because high lake levels in Coeur d?Alene Lake caused backwater conditions. Average simulated velocities along the thalweg ranged from about 3 to 5.3 feet per second, and maximum simulated velocities ranged from 3.9 to 7 feet per second. In the dredged reach, average simulated velocity along the thalweg ranged from 3.5 to 6 feet per second. The model also simulated several back-eddies (flow reversal); the largest eddy encompassed about one-third of the river width. Average bed shear stresses increased more than 200 percent from the first to the last simulation. Simulated sediment mobility, asses

A 40 GHz fully integrated circuit with a vector network analyzer and a coplanar-line-based detection area for circulating tumor cell analysis using 65 nm CMOS technology

NASA Astrophysics Data System (ADS)

Nakanishi, Taiki; Matsunaga, Maya; Kobayashi, Atsuki; Nakazato, Kazuo; Niitsu, Kiichi

2018-03-01

A 40-GHz fully integrated CMOS-based circuit for circulating tumor cells (CTC) analysis, consisting of an on-chip vector network analyzer (VNA) and a highly sensitive coplanar-line-based detection area is presented in this paper. In this work, we introduce a fully integrated architecture that eliminates unwanted parasitic effects. The proposed analyzer was designed using 65 nm CMOS technology, and SPICE and MWS simulations were used to validate its operation. The simulation confirmed that the proposed circuit can measure S-parameter shifts resulting from the addition of various types of tumor cells to the detection area, the data of which are provided in a previous study: the |S 21| values for HepG2, A549, and HEC-1-A cells are -0.683, -0.580, and -0.623 dB, respectively. Additionally, the measurement demonstrated an S-parameters reduction of -25.7% when a silicone resin was put on the circuit. Hence, the proposed system is expected to contribute to cancer diagnosis.

Soil Moisture Content Estimation Based on Sentinel-1 and Auxiliary Earth Observation Products. A Hydrological Approach

PubMed Central

Alexakis, Dimitrios D.; Mexis, Filippos-Dimitrios K.; Vozinaki, Anthi-Eirini K.; Daliakopoulos, Ioannis N.; Tsanis, Ioannis K.

2017-01-01

A methodology for elaborating multi-temporal Sentinel-1 and Landsat 8 satellite images for estimating topsoil Soil Moisture Content (SMC) to support hydrological simulation studies is proposed. After pre-processing the remote sensing data, backscattering coefficient, Normalized Difference Vegetation Index (NDVI), thermal infrared temperature and incidence angle parameters are assessed for their potential to infer ground measurements of SMC, collected at the top 5 cm. A non-linear approach using Artificial Neural Networks (ANNs) is tested. The methodology is applied in Western Crete, Greece, where a SMC gauge network was deployed during 2015. The performance of the proposed algorithm is evaluated using leave-one-out cross validation and sensitivity analysis. ANNs prove to be the most efficient in SMC estimation yielding R2 values between 0.7 and 0.9. The proposed methodology is used to support a hydrological simulation with the HEC-HMS model, applied at the Keramianos basin which is ungauged for SMC. Results and model sensitivity highlight the contribution of combining Sentinel-1 SAR and Landsat 8 images for improving SMC estimates and supporting hydrological studies. PMID:28635625

Soil Moisture Content Estimation Based on Sentinel-1 and Auxiliary Earth Observation Products. A Hydrological Approach.

PubMed

Alexakis, Dimitrios D; Mexis, Filippos-Dimitrios K; Vozinaki, Anthi-Eirini K; Daliakopoulos, Ioannis N; Tsanis, Ioannis K

2017-06-21

A methodology for elaborating multi-temporal Sentinel-1 and Landsat 8 satellite images for estimating topsoil Soil Moisture Content (SMC) to support hydrological simulation studies is proposed. After pre-processing the remote sensing data, backscattering coefficient, Normalized Difference Vegetation Index (NDVI), thermal infrared temperature and incidence angle parameters are assessed for their potential to infer ground measurements of SMC, collected at the top 5 cm. A non-linear approach using Artificial Neural Networks (ANNs) is tested. The methodology is applied in Western Crete, Greece, where a SMC gauge network was deployed during 2015. The performance of the proposed algorithm is evaluated using leave-one-out cross validation and sensitivity analysis. ANNs prove to be the most efficient in SMC estimation yielding R² values between 0.7 and 0.9. The proposed methodology is used to support a hydrological simulation with the HEC-HMS model, applied at the Keramianos basin which is ungauged for SMC. Results and model sensitivity highlight the contribution of combining Sentinel-1 SAR and Landsat 8 images for improving SMC estimates and supporting hydrological studies.

Evaluating the effects of dam breach methodologies on Consequence Estimation through Sensitivity Analysis

NASA Astrophysics Data System (ADS)

Kalyanapu, A. J.; Thames, B. A.

2013-12-01

Dam breach modeling often includes application of models that are sophisticated, yet computationally intensive to compute flood propagation at high temporal and spatial resolutions. This results in a significant need for computational capacity that requires development of newer flood models using multi-processor and graphics processing techniques. Recently, a comprehensive benchmark exercise titled the 12th Benchmark Workshop on Numerical Analysis of Dams, is organized by the International Commission on Large Dams (ICOLD) to evaluate the performance of these various tools used for dam break risk assessment. The ICOLD workshop is focused on estimating the consequences of failure of a hypothetical dam near a hypothetical populated area with complex demographics, and economic activity. The current study uses this hypothetical case study and focuses on evaluating the effects of dam breach methodologies on consequence estimation and analysis. The current study uses ICOLD hypothetical data including the topography, dam geometric and construction information, land use/land cover data along with socio-economic and demographic data. The objective of this study is to evaluate impacts of using four different dam breach methods on the consequence estimates used in the risk assessments. The four methodologies used are: i) Froehlich (1995), ii) MacDonald and Langridge-Monopolis 1984 (MLM), iii) Von Thun and Gillete 1990 (VTG), and iv) Froehlich (2008). To achieve this objective, three different modeling components were used. First, using the HEC-RAS v.4.1, dam breach discharge hydrographs are developed. These hydrographs are then provided as flow inputs into a two dimensional flood model named Flood2D-GPU, which leverages the computer's graphics card for much improved computational capabilities of the model input. Lastly, outputs from Flood2D-GPU, including inundated areas, depth grids, velocity grids, and flood wave arrival time grids, are input into HEC-FIA, which provides the consequence assessment for the solution to the problem statement. For the four breach methodologies, a sensitivity analysis of four breach parameters, breach side slope (SS), breach width (Wb), breach invert elevation (Elb), and time of failure (tf), is conducted. Up to, 68 simulations are computed to produce breach hydrographs in HEC-RAS for input into Flood2D-GPU. The Flood2D-GPU simulation results were then post-processed in HEC-FIA to evaluate: Total Population at Risk (PAR), 14-yr and Under PAR (PAR14-), 65-yr and Over PAR (PAR65+), Loss of Life (LOL) and Direct Economic Impact (DEI). The MLM approach resulted in wide variability in simulated minimum and maximum values of PAR, PAR 65+ and LOL estimates. For PAR14- and DEI, Froehlich (1995) resulted in lower values while MLM resulted in higher estimates. This preliminary study demonstrated the relative performance of four commonly used dam breach methodologies and their impacts on consequence estimation.

A Regev-type fully homomorphic encryption scheme using modulus switching.

PubMed

Chen, Zhigang; Wang, Jian; Chen, Liqun; Song, Xinxia

2014-01-01

A critical challenge in a fully homomorphic encryption (FHE) scheme is to manage noise. Modulus switching technique is currently the most efficient noise management technique. When using the modulus switching technique to design and implement a FHE scheme, how to choose concrete parameters is an important step, but to our best knowledge, this step has drawn very little attention to the existing FHE researches in the literature. The contributions of this paper are twofold. On one hand, we propose a function of the lower bound of dimension value in the switching techniques depending on the LWE specific security levels. On the other hand, as a case study, we modify the Brakerski FHE scheme (in Crypto 2012) by using the modulus switching technique. We recommend concrete parameter values of our proposed scheme and provide security analysis. Our result shows that the modified FHE scheme is more efficient than the original Brakerski scheme in the same security level.

Robust Prediction of Hydraulic Roughness

DTIC Science & Technology

2011-03-01

Manning’s n were required as input for further hydraulic analyses with HEC - RAS . HYDROCAL was applied to compare different estimates of resistance... River Restoration Science Synthesis (NRRSS) demonstrated that, in 2007, river and stream restoration projects and funding were at an all time high...behavior makes this parameter very difficult to quan- tify repeatedly and accurately. A fundamental concept of hydraulic theory in the context of river

The cascade high productivity language

NASA Technical Reports Server (NTRS)

Callahan, David; Chamberlain, Branford L.; Zima, Hans P.

2004-01-01

This paper describes the design of Chapel, the Cascade High Productivity Language, which is being developed in the DARPA-funded HPCS project Cascade led by Cray Inc. Chapel pushes the state-of-the-art in languages for HEC system programming by focusing on productivity, in particular by combining the goal of highest possible object code performance with that of programmability offered by a high-level user interface.

USACE Regional Sediment Management and Engineering with Nature 2013 Workshop Summary

DTIC Science & Technology

2014-08-01

Tools into HEC-RAS St. Louis Kaskaskia River Navigation Channel Mobile Biodegradable Containment Structures for RSM Honolulu Hawaii RSM – Haleiwa...sediment from USACE to private entities for use. Share successes and lessons learned.  Increase the use of vegetation and other biodegradable ...beaches, and submerged mounds) and construction materials (e.g., reef balls, combination of rock sills and oyster bags , native plants, and

Optimum Selection of Clustered Conservation Areas Within Military Installations

DTIC Science & Technology

2011-10-01

Installations Co ns tr uc tio n En gi ne er in g R es ea rc h La bo ra to ry Sahan T. M. Dissanayake, Hayri Önal, James D. Westervelt, and Harold...with this grid cell value to reflect the sustainable number of GTs for each CMA. A 1 hec - tare land parcel can support between 2 to 4 GTs. This is

Divergent migration within lake sturgeon (Acipenser fulvescens) populations: Multiple distinct patterns exist across an unrestricted migration corridor.

PubMed

Kessel, Steven T; Hondorp, Darryl W; Holbrook, Christopher M; Boase, James C; Chiotti, Justin A; Thomas, Michael V; Wills, Todd C; Roseman, Edward F; Drouin, Richard; Krueger, Charles C

2018-01-01

Population structure, distribution, abundance and dispersal arguably underpin the entire field of animal ecology, with consequences for regional species persistence, and provision of ecosystem services. Divergent migration behaviours among individuals or among populations are an important aspect of the ecology of highly mobile animals, allowing populations to exploit spatially or temporally distributed food and space resources. This study investigated the spatial ecology of lake sturgeon (Acipenser fulvescens) within the barrier free Huron-Erie Corridor (HEC), which connects Lake Huron and Lake Erie of the North American Laurentian Great Lakes. Over 6 years (2011-2016), movements of 268 lake sturgeon in the HEC were continuously monitored across the Great Lakes using acoustic telemetry (10 years battery life acoustic transmitters). Five distinct migration behaviours were identified with hierarchical cluster analysis, based on the phenology and duration of river and lake use. Lake sturgeon in the HEC were found to contain a high level of intraspecific divergent migration, including partial migration with the existence of residents. Specific behaviours included year-round river residency and multiple lake-migrant behaviours that involved movements between lakes and rivers. Over 85% of individuals were assigned to migration behaviours as movements were consistently repeated over the study, which suggested migration behaviours were consistent and persistent in lake sturgeon. Differential use of specific rivers or lakes by acoustic-tagged lake sturgeon further subdivided individuals into 14 "contingents" (spatiotemporally segregated subgroups). Contingents associated with one river (Detroit or St. Clair) were rarely detected in the other river, which confirmed that lake sturgeon in the Detroit and St. Clair represent two semi-independent populations that could require separate management consideration for their conservation. The distribution of migration behaviours did not vary between populations, sexes, body size or among release locations, which indicated that intrapopulation variability in migratory behaviour is a general feature of the spatial ecology of lake sturgeon in unfragmented landscapes. 2017 The Authors. Journal of Animal Ecology published by John Wiley & Sons Ltd on behalf of British Ecological Society.

Asymmetrical flow field-flow fractionation coupled to inductively coupled plasma mass spectrometry for sizing SeNPs for packaging applications

NASA Astrophysics Data System (ADS)

Palomo-Siguero, María; Vera, Paula; Echegoyen, Yolanda; Nerin, Cristina; Cámara, Carmen; Madrid, Yolanda

2017-06-01

This paper describes the application of Asymmetrical Flow Field-Flow Fractionation (AF4) coupled to diode array detector (DAD) and inductively coupled plasma mass spectrometry (AF4-UV-ICP-MS) to characterize selenium nanoparticles (SeNPs) in an aqueous acrylic adhesive to be used in a multilayer food packaging material. SeNPs were synthesized using a solution-phase approach based on the reduction of selenite with ascorbic acid in presence of different stabilizers compatible with food industry such as polysaccharides (chitosan (poly(D-glucosamine) and hydroxyethylcellulose (HEC)) and non-ionic surfactants (Triton X-100 (t-octylphenoxypolyethoxyethanol), 2,4,7,9-tetramethyl 5decyne-4,7-diol ethoxylate, and isotridecanol ethoxylate). Several parameters such as pH, ascorbic acid and stabilizers concentration, and compatibility of the stabilizer with the adhesive were evaluated. SeNPs suspensions with spherical morphology were obtained except when isotridecanol ethoxylate was employed which provides SeNPs with a nanorod morphology. AF4-DAD-ICP-MS was further applied for sizing the different SeNPs preparations. DAD was used as detector for selecting the best AF4 separation conditions before coupling to ICP-MS to ensure unequivocal identification of NPs. AF4 calibration with polystyrene latex (PSL) beads of known sizes allowed size determination of the different SeNPs. The following estimated hydrodynamic sizes (expressed as the mean ± standard deviation, n = 6 replicates) were found: chitosan-SeNPs- (26 ± 3 nm), TritonX100-SeNPs (22 ± 10 nm) HEC- SeNPs (91 ± 8 nm) and 2,4,7,9-tetramethyl 5decyne-4,7-diol ethoxylate- SeNPs (59 ± 4 nm). The proposed methodology was successfully applied to the characterization in terms of size of aqueous acrylic adhesives containing SeNPs Results from AF4-ICP-MS and TEM shown that only those SeNPs obtained with non-ionic surfactants and HEC were compatible with the adhesive. The results reported here evidence the usefulness of AF4-ICP-MS as analytical tool for controlling those manufacturing process involving nanoparticles which opens new frontiers in applicability of AF4-ICP-MS.

On Space-Time Inversion Invariance and its Relation to Non-Dissipatedness of a CESE Core Scheme

NASA Technical Reports Server (NTRS)

Chang, Sin-Chung

2006-01-01

The core motivating ideas of the space-time CESE method are clearly presented and critically analyzed. It is explained why these ideas result in all the simplifying and enabling features of the CESE method. A thorough discussion of the a scheme, a two-level non-dissipative CESE solver of a simple advection equation with two independent mesh variables and two equations per mesh point is also presented. It is shown that the scheme possesses some rather intriguing properties such as: (i) its two independent mesh variables separately satisfy two decoupled three-level leapfrog schemes and (ii) it shares with the leapfrog scheme the same amplification factors, even though the a scheme and the leapfrog scheme have completely different origins and structures. It is also explained why the leapfrog scheme is not as robust as the a scheme. The amplification factors/matrices of several non-dissipative schemes are carefully studied and the key properties that contribute to their non-dissipatedness are clearly spelled out. Finally we define and establish space-time inversion (STI) invariance for several non-dissipative schemes and show that their non-dissipatedness is a result of their STI invariance.

New Imaging Operation Scheme at VLTI

NASA Astrophysics Data System (ADS)

Haubois, Xavier

2018-04-01

After PIONIER and GRAVITY, MATISSE will soon complete the set of 4 telescope beam combiners at VLTI. Together with recent developments in the image reconstruction algorithms, the VLTI aims to develop its operation scheme to allow optimized and adaptive UV plane coverage. The combination of spectro-imaging instruments, optimized operation framework and image reconstruction algorithms should lead to an increase of the reliability and quantity of the interferometric images. In this contribution, I will present the status of this new scheme as well as possible synergies with other instruments.

A subtraction scheme for computing QCD jet cross sections at NNLO: integrating the doubly unresolved subtraction terms

NASA Astrophysics Data System (ADS)

Somogyi, Gábor

2013-04-01

We finish the definition of a subtraction scheme for computing NNLO corrections to QCD jet cross sections. In particular, we perform the integration of the soft-type contributions to the doubly unresolved counterterms via the method of Mellin-Barnes representations. With these final ingredients in place, the definition of the scheme is complete and the computation of fully differential rates for electron-positron annihilation into two and three jets at NNLO accuracy becomes feasible.

Employer contribution and premium growth in health insurance.

PubMed

Liu, Yiyan; Jin, Ginger Zhe

2015-01-01

We study whether employer premium contribution schemes could impact the pricing behavior of health plans and contribute to rising premiums. Using 1991-2011 data before and after a 1999 premium subsidy policy change in the Federal Employees Health Benefits Program (FEHBP), we find that the employer premium contribution scheme has a differential impact on health plan pricing based on two market incentives: 1) consumers are less price sensitive when they only need to pay part of the premium increase, and 2) each health plan has an incentive to increase the employer's premium contribution to that plan. Both incentives are found to contribute to premium growth. Counterfactual simulation shows that average premium would have been 10% less than observed and the federal government would have saved 15% per year on its premium contribution had the subsidy policy change not occurred in the FEHBP. We discuss the potential of similar incentives in other government-subsidized insurance systems such as the Medicare Part D and the Health Insurance Marketplace under the Affordable Care Act. Copyright © 2014 Elsevier B.V. All rights reserved.

Possible stimuli for strength and power adaptation : acute metabolic responses.

PubMed

Crewther, Blair; Cronin, John; Keogh, Justin

2006-01-01

The metabolic response to resistance exercise, in particular lactic acid or lactate, has a marked influence upon the muscular environment, which may enhance the training stimulus (e.g. motor unit activation, hormones or muscle damage) and thereby contribute to strength and power adaptation. Hypertrophy schemes have resulted in greater lactate responses (%) than neuronal and dynamic power schemes, suggesting possible metabolic-mediated changes in muscle growth. Factors such as age, sex, training experience and nutrition may also influence the lactate responses to resistance exercise and thereafter, muscular adaptation. Although the importance of the mechanical and hormonal stimulus to strength and power adaptation is well recognised, the contribution of the metabolic stimulus is largely unknown. Relatively few studies for example, have examined metabolic change across neuronal and dynamic power schemes, and not withstanding the fact that those mechanisms underpinning muscular adaptation, in relation to the metabolic stimulus, remain highly speculative. Inconsistent findings and methodological limitations within research (e.g. programme design, sampling period, number of samples) make interpretation further difficult. We contend that strength and power research needs to investigate those metabolic mechanisms likely to contribute to weight-training adaptation. Further research is also needed to examine the metabolic responses to different loading schemes, as well as interactions across age, sex and training status, so our understanding of how to optimise strength and power development is improved.

Content Analysis Coding Schemes for Online Asynchronous Discussion

ERIC Educational Resources Information Center

Weltzer-Ward, Lisa

2011-01-01

Purpose: Researchers commonly utilize coding-based analysis of classroom asynchronous discussion contributions as part of studies of online learning and instruction. However, this analysis is inconsistent from study to study with over 50 coding schemes and procedures applied in the last eight years. The aim of this article is to provide a basis…

Costs and economic consequences of a help-at-home scheme for older people in England.

PubMed

Bauer, Annette; Knapp, Martin; Wistow, Gerald; Perkins, Margaret; King, Derek; Iemmi, Valentina

2017-03-01

Solutions to support older people to live independently and reduce the cost of an ageing population are high on the political agenda of most developed countries. Help-at-home schemes offer a mix of community support with the aim to address a range of well-being needs. However, not much is currently known about the costs, outcomes and economic consequences of such schemes. Understanding their impact on individuals' well-being and the economic consequences for local and central government can contribute to decisions about sustainable long-term care financing. This article presents results from a mixed-methods study of a voluntary sector-provided help-at-home scheme in England for people of 55 years and older. The study followed a participatory approach, which involved staff and volunteers. Data were collected during 2012 and 2013. Social care-related quality of life was measured with the Adult Social Care Outcomes Toolkit for 24 service users (59% response rate) when they started using the scheme and 4-6 months later. A customised questionnaire that captured resource use and well-being information was sent to 1064 service users (63% response rate). The same tool was used in assessment with service users who started using the scheme between November 2012 and April 2013 (100% response rate). Costs of the scheme were established from local budget and activity data. The scheme was likely to achieve a mean net benefit of £1568 per person from a local government and National Health Service (NHS) perspective and £3766 from the perspective of the individual. An expenditure of £2851 per person accrued to central government for the additional redistribution of benefit payments to older people. This article highlights the potential contribution of voluntary sector-run help-at-home schemes to an affordable welfare system for ageing societies. © 2016 John Wiley & Sons Ltd.

The Politico-Economic Challenges of Ghana’s National Health Insurance Scheme Implementation

PubMed Central

Fusheini, Adam

2016-01-01

Background: National/social health insurance schemes have increasingly been seen in many low- and middle-income countries (LMICs) as a vehicle to universal health coverage (UHC) and a viable alternative funding mechanism for the health sector. Several countries, including Ghana, have thus introduced and implemented mandatory national health insurance schemes (NHIS) as part of reform efforts towards increasing access to health services. Ghana passed mandatory national health insurance (NHI) legislation (ACT 650) in 2003 and commenced nationwide implementation in 2004. Several peer review studies and other research reports have since assessed the performance of the scheme with positive rating while challenges also noted. This paper contributes to the literature on economic and political implementation challenges based on empirical evidence from the perspectives of the different category of actors and institutions involved in the process. Methods: Qualitative in-depth interviews were held with 33 different category of participants in four selected district mutual health insurance schemes in Southern (two) and Northern (two) Ghana. This was to ascertain their views regarding the main challenges in the implementation process. The participants were selected through purposeful sampling, stakeholder mapping, and snowballing. Data was analysed using thematic grouping procedure. Results: Participants identified political issues of over politicisation and political interference as main challenges. The main economic issues participants identified included low premiums or contributions; broad exemptions, poor gatekeeper enforcement system; and culture of curative and hospital-centric care. Conclusion: The study establishes that political and economic factors have influenced the implementation process and the degree to which the policy has been implemented as intended. Thus, we conclude that there is a synergy between implementation and politics; and achieving UHC under the NHIS requires political stewardship. Political leadership has the responsibility to build trust and confidence in the system by providing the necessary resources and backing with minimal interference in the operations. For sustainability of the scheme, authorities need to review the exemption policy, rate of contributions, especially, from informal sector employees and recruitment criteria of scheme workers, explore additional sources of funding and re-examine training needs of employees to strengthen their competences among others. PMID:27694681

The Politico-Economic Challenges of Ghana's National Health Insurance Scheme Implementation.

PubMed

Fusheini, Adam

2016-04-27

National/social health insurance schemes have increasingly been seen in many low- and middle-income countries (LMICs) as a vehicle to universal health coverage (UHC) and a viable alternative funding mechanism for the health sector. Several countries, including Ghana, have thus introduced and implemented mandatory national health insurance schemes (NHIS) as part of reform efforts towards increasing access to health services. Ghana passed mandatory national health insurance (NHI) legislation (ACT 650) in 2003 and commenced nationwide implementation in 2004. Several peer review studies and other research reports have since assessed the performance of the scheme with positive rating while challenges also noted. This paper contributes to the literature on economic and political implementation challenges based on empirical evidence from the perspectives of the different category of actors and institutions involved in the process. Qualitative in-depth interviews were held with 33 different category of participants in four selected district mutual health insurance schemes in Southern (two) and Northern (two) Ghana. This was to ascertain their views regarding the main challenges in the implementation process. The participants were selected through purposeful sampling, stakeholder mapping, and snowballing. Data was analysed using thematic grouping procedure. Participants identified political issues of over politicisation and political interference as main challenges. The main economic issues participants identified included low premiums or contributions; broad exemptions, poor gatekeeper enforcement system; and culture of curative and hospital-centric care. The study establishes that political and economic factors have influenced the implementation process and the degree to which the policy has been implemented as intended. Thus, we conclude that there is a synergy between implementation and politics; and achieving UHC under the NHIS requires political stewardship. Political leadership has the responsibility to build trust and confidence in the system by providing the necessary resources and backing with minimal interference in the operations. For sustainability of the scheme, authorities need to review the exemption policy, rate of contributions, especially, from informal sector employees and recruitment criteria of scheme workers, explore additional sources of funding and re-examine training needs of employees to strengthen their competences among others. © 2016 by Kerman University of Medical Sciences

Simple aerosol correction technique based on the spectral relationships of the aerosol multiple-scattering reflectances for atmospheric correction over the oceans.

PubMed

Ahn, Jae-Hyun; Park, Young-Je; Kim, Wonkook; Lee, Boram

2016-12-26

An estimation of the aerosol multiple-scattering reflectance is an important part of the atmospheric correction procedure in satellite ocean color data processing. Most commonly, the utilization of two near-infrared (NIR) bands to estimate the aerosol optical properties has been adopted for the estimation of the effects of aerosols. Previously, the operational Geostationary Color Ocean Imager (GOCI) atmospheric correction scheme relies on a single-scattering reflectance ratio (SSE), which was developed for the processing of the Sea-viewing Wide Field-of-view Sensor (SeaWiFS) data to determine the appropriate aerosol models and their aerosol optical thicknesses. The scheme computes reflectance contributions (weighting factor) of candidate aerosol models in a single scattering domain then spectrally extrapolates the single-scattering aerosol reflectance from NIR to visible (VIS) bands using the SSE. However, it directly applies the weight value to all wavelengths in a multiple-scattering domain although the multiple-scattering aerosol reflectance has a non-linear relationship with the single-scattering reflectance and inter-band relationship of multiple scattering aerosol reflectances is non-linear. To avoid these issues, we propose an alternative scheme for estimating the aerosol reflectance that uses the spectral relationships in the aerosol multiple-scattering reflectance between different wavelengths (called SRAMS). The process directly calculates the multiple-scattering reflectance contributions in NIR with no residual errors for selected aerosol models. Then it spectrally extrapolates the reflectance contribution from NIR to visible bands for each selected model using the SRAMS. To assess the performance of the algorithm regarding the errors in the water reflectance at the surface or remote-sensing reflectance retrieval, we compared the SRAMS atmospheric correction results with the SSE atmospheric correction using both simulations and in situ match-ups with the GOCI data. From simulations, the mean errors for bands from 412 to 555 nm were 5.2% for the SRAMS scheme and 11.5% for SSE scheme in case-I waters. From in situ match-ups, 16.5% for the SRAMS scheme and 17.6% scheme for the SSE scheme in both case-I and case-II waters. Although we applied the SRAMS algorithm to the GOCI, it can be applied to other ocean color sensors which have two NIR wavelengths.

Ten Years in the Making: AUSM-Family

NASA Technical Reports Server (NTRS)

Liou, Meng-Sing

2001-01-01

We begin by describing the motivations that gave birth to the original AUSM scheme and then focus on the ingredients that has spurred its growth and acceptance by the world of computational fluid dynamics. As it has played out more in the field, weaknesses have also surfaced. Hence, nutrients and supplements are prescribed to help it grow and stay strong and robust. In this paper, we will describe the saga of efforts owing to researchers who have contributed to building up the AUSM-family for the CFD community. It is hoped that a healthy scheme will contribute to the accurate and robust solution of problems encountered in a wide range of disciplines. We analyze numerical mass fluxes with an emphasis on their capability for accurately capturing shock and contact discontinuities. We will present a new scheme for the pressure flux, along with results for a host of test problems.

Security-Oriented and Load-Balancing Wireless Data Routing Game in the Integration of Advanced Metering Infrastructure Network in Smart Grid

DOE Office of Scientific and Technical Information (OSTI.GOV)

He, Fulin; Cao, Yang; Zhang, Jun Jason

Ensuring flexible and reliable data routing is indispensable for the integration of Advanced Metering Infrastructure (AMI) networks, we propose a secure-oriented and load-balancing wireless data routing scheme. A novel utility function is designed based on security routing scheme. Then, we model the interactive security-oriented routing strategy among meter data concentrators or smart grid meters as a mixed-strategy network formation game. Finally, such problem results in a stable probabilistic routing scheme with proposed distributed learning algorithm. One contributions is that we studied that different types of applications affect the routing selection strategy and the strategy tendency. Another contributions is that themore » chosen strategy of our mixed routing can adaptively to converge to a new mixed strategy Nash equilibrium (MSNE) during the learning process in the smart grid.« less

Near East/North Africa Report, Number 2603

DTIC Science & Technology

1982-08-17

Electricity Stations 118 Gum Arabic Belt 118 Popular Development 118 Foreign Exchange Regulating Measures 118 Tombura on HEC Policy 119...Middle East and that this underlines the need for a just solution to the Palestinian problem—a solution that guar - antees the legitimate rights of the...last months of 1360. It must be noted that prices of a number of goods & services, such as cold drinks, washing powder , some cigarettes and telephone

Methodology for predicting maximum velocity and shear stress in a sinuous channel with bendway weirs using 1-D HEC-RAS modeling results

Treesearch

Paul Sclafani; Christopher I. Thornton; Amanda L. Cox; Steven R. Abt

2012-01-01

As with every natural process, river morphology incorporates a complex and nuanced set of processes that combine to produce an infinite set of possible outcomes. Natural laws that govern natural systems cause rivers to react to changes in the environment in order to maintain equilibrium. Changes in hydraulics, sediment transport, and habitat help keep natural systems...

Assessment of insulin sensitivity by the hyperinsulinemic euglycemic clamp: Comparison with the spectral analysis of photoplethysmography.

PubMed

De Souza, Aglecio Luiz; Batista, Gisele Almeida; Alegre, Sarah Monte

2017-01-01

We compare spectral analysis of photoplethysmography (PTG) with insulin resistance measured by the hyperinsulinemic euglycemic clamp (HEC) technique. A total of 100 nondiabetic subjects, 43 men and 57 women aged 20-63years, 30 lean, 42 overweight and 28 obese were enrolled in the study. These patients underwent an examination with HEC, and an examination with the PTG spectral analysis and calculation of the PTG Total Power (PTG-TP). Receiver-operating characteristic (ROC) curves were constructed to determine the specificity and sensitivity of PTG-TP in the assessment of insulin resistance. There is a moderate correlation between insulin sensitivity (M-value) and PTG-TP (r=- 0.64, p<0.0001). The ROC curves showed that the most relevant cutoff to the whole study group was a PTG-TP>406.2. This cut-off had a sensitivity=95.7%, specificity =84,4% and the area under the ROC curve (AUC)=0.929 for identifying insulin resistance. All AUC ROC curve analysis were significant (p<0.0001). The use of the PTG-TP marker measured from the PTG spectral analysis is a useful tool in screening and follow up of IR, especially in large-scale studies. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

Water quality modeling in the systems impact assessment model for the Klamath River basin - Keno, Oregon to Seiad Valley, California

USGS Publications Warehouse

Hanna, R. Blair; Campbell, Sharon G.

2000-01-01

This report describes the water quality model developed for the Klamath River System Impact Assessment Model (SIAM). The Klamath River SIAM is a decision support system developed by the authors and other US Geological Survey (USGS), Midcontinent Ecological Science Center staff to study the effects of basin-wide water management decisions on anadromous fish in the Klamath River. The Army Corps of Engineersa?? HEC5Q water quality modeling software was used to simulate water temperature, dissolved oxygen and conductivity in 100 miles of the Klamath River Basin in Oregon and California. The water quality model simulated three reservoirs and the mainstem Klamath River influenced by the Shasta and Scott River tributaries. Model development, calibration and two validation exercises are described as well as the integration of the water quality model into the SIAM decision support system software. Within SIAM, data are exchanged between the water quantity model (MODSIM), the water quality model (HEC5Q), the salmon population model (SALMOD) and methods for evaluating ecosystem health. The overall predictive ability of the water quality model is described in the context of calibration and validation error statistics. Applications of SIAM and the water quality model are described.

Carbon coated nano-LiTi2(PO4)3 electrodes for non-aqueous hybrid supercapacitors.

PubMed

Aravindan, V; Chuiling, W; Reddy, M V; Rao, G V Subba; Chowdari, B V R; Madhavi, S

2012-04-28

The Pechini type polymerizable complex decomposition method is employed to prepare LiTi(2)(PO(4))(3) at 1000 °C in air. High energy ball milling followed by carbon coating by the glucose-method yielded C-coated nano-LiTi(2)(PO(4))(3) (LTP) with a crystallite size of 80(±5) nm. The phase is characterized by X-ray diffraction, Rietveld refinement, thermogravimetry, SEM, HR-TEM and Raman spectra. Lithium cycling properties of LTP show that 1.75 moles of Li (~121 mA h g(-1) at 15 mA g(-1) current) per formula unit can be reversibly cycled between 2 and 3.4 V vs. Li with 83% capacity retention after 70 cycles. Cyclic voltammograms (CV) reveal the two-phase reaction mechanism during Li insertion/extraction. A hybrid electrochemical supercapacitor (HEC) with LTP as negative electrode and activated carbon (AC) as positive electrode in non-aqueous electrolyte is studied by CV at various scan rates and by galvanostatic cycling at various current rates up to 1000 cycles in the range 0-3 V. Results show that the HEC delivers a maximum energy density of 14 W h kg(-1) and a power density of 180 W kg(-1). This journal is © the Owner Societies 2012

Improving a DSM Obtained by Unmanned Aerial Vehicles for Flood Modelling

NASA Astrophysics Data System (ADS)

Mourato, Sandra; Fernandez, Paulo; Pereira, Luísa; Moreira, Madalena

2017-12-01

According to the EU flood risks directive, flood hazard map must be used to assess the flood risk. These maps can be developed with hydraulic modelling tools using a Digital Surface Runoff Model (DSRM). During the last decade, important evolutions of the spatial data processing has been developed which will certainly improve the hydraulic models results. Currently, images acquired with Red/Green/Blue (RGB) camera transported by Unmanned Aerial Vehicles (UAV) are seen as a good alternative data sources to represent the terrain surface with a high level of resolution and precision. The question is if the digital surface model obtain with this data is adequate enough for a good representation of the hydraulics flood characteristics. For this purpose, the hydraulic model HEC-RAS was run with 4 different DSRM for an 8.5 km reach of the Lis River in Portugal. The computational performance of the 4 modelling implementations is evaluated. Two hydrometric stations water level records were used as boundary conditions of the hydraulic model. The records from a third hydrometric station were used to validate the optimal DSRM. The HEC-RAS results had the best performance during the validation step were the ones where the DSRM with integration of the two altimetry data sources.

Stream profile analysis using a step backwater model for selected reaches in the Chippewa Creek basin in Medina, Wayne, and Summit Counties, Ohio

USGS Publications Warehouse

Straub, David E.; Ebner, Andrew D.

2011-01-01

The USGS, in cooperation with the Chippewa Subdistrict of the Muskingum Watershed Conservancy District, performed hydrologic and hydraulic analyses for selected reaches of three streams in Medina, Wayne, Stark, and Summit Counties in northeast Ohio: Chippewa Creek, Little Chippewa Creek, and River Styx. This study was done to facilitate assessment of various alternatives for mitigating flood hazards in the Chippewa Creek basin. StreamStats regional regression equations were used to estimate instantaneous peak discharges approximately corresponding to bankfull flows. Explanatory variables used in the regression equations were drainage area, main-channel slope, and storage area. Hydraulic models were developed to determine water-surface profiles along the three stream reaches studied for the bankfull discharges established in the hydrologic analyses. The HEC-RAS step-backwater hydraulic analysis model was used to determine water-surface profiles for the three streams. Starting water-surface elevations for all streams were established using normal depth computations in the HEC-RAS models. Cross-sectional elevation data, hydraulic-structure geometries, and roughness coefficients were collected in the field and (along with peak-discharge estimates) used as input for the models. Reach-averaged reductions in water-surface elevations ranged from 0.11 to 1.29 feet over the four roughness coefficient reduction scenarios.

Autoantibodies Targeting AT1 Receptor from Patients with Acute Coronary Syndrome Upregulate Proinflammatory Cytokines Expression in Endothelial Cells Involving NF-κB Pathway

PubMed Central

Li, Weijuan; Li, Zhi; Chen, Yaoqi; Li, Songhai; Lv, Yuanyuan; Zhou, Wenping; Liao, Mengyang; Zhu, Feng; Zhou, Zihua; Cheng, Xiang; Zeng, Qiutang; Liao, Yuhua; Wei, Yumiao

2014-01-01

Our study intended to prove whether agonistic autoantibodies to angiotensin II type 1 receptor (AT1-AAs) exist in patients with coronary heart disease (CHD) and affect the human endothelial cell (HEC) by upregulating proinflammatory cytokines expression involved in NF-κB pathway. Antibodies were determined by chronotropic responses of cultured neonatal rat cardiomyocytes coupled with receptor-specific antagonists (valsartan and AT1-EC2) as described previously. Interleukin-6 (IL-6), vascular cell adhesion molecule-1 (VCAM-1), and monocyte chemotactic protein-1 (MCP-1) expression were improved at both mRNA and protein levels in HEC, while NF-κB in the DNA level was improved detected by electrophoretic mobility shift assays (EMSA). These improvements could be inhibited by specific AT1 receptor blocker valsartan, NF-κB blocker pyrrolidine dithiocarbamate (PDTC), and specific short peptides from the second extracellular loop of AT1 receptor. These results suggested that AT1-AAs, via the AT1 receptor, induce expression of proinflammatory cytokines involved in the activation of NF-κB. AT1-AAs may play a great role in the pathogenesis of the acute coronary syndrome by mediating vascular inflammatory effects involved in the NF-κB pathway. PMID:25762441

Autoantibodies targeting AT1 receptor from patients with acute coronary syndrome upregulate proinflammatory cytokines expression in endothelial cells involving NF-κB pathway.

PubMed

Li, Weijuan; Li, Zhi; Chen, Yaoqi; Li, Songhai; Lv, Yuanyuan; Zhou, Wenping; Liao, Mengyang; Zhu, Feng; Zhou, Zihua; Cheng, Xiang; Zeng, Qiutang; Liao, Yuhua; Wei, Yumiao

2014-01-01

Our study intended to prove whether agonistic autoantibodies to angiotensin II type 1 receptor (AT1-AAs) exist in patients with coronary heart disease (CHD) and affect the human endothelial cell (HEC) by upregulating proinflammatory cytokines expression involved in NF-κB pathway. Antibodies were determined by chronotropic responses of cultured neonatal rat cardiomyocytes coupled with receptor-specific antagonists (valsartan and AT1-EC2) as described previously. Interleukin-6 (IL-6), vascular cell adhesion molecule-1 (VCAM-1), and monocyte chemotactic protein-1 (MCP-1) expression were improved at both mRNA and protein levels in HEC, while NF-κB in the DNA level was improved detected by electrophoretic mobility shift assays (EMSA). These improvements could be inhibited by specific AT1 receptor blocker valsartan, NF-κB blocker pyrrolidine dithiocarbamate (PDTC), and specific short peptides from the second extracellular loop of AT1 receptor. These results suggested that AT1-AAs, via the AT1 receptor, induce expression of proinflammatory cytokines involved in the activation of NF-κB. AT1-AAs may play a great role in the pathogenesis of the acute coronary syndrome by mediating vascular inflammatory effects involved in the NF-κB pathway.

Examining the effects of urban agglomeration polders on flood events in Qinhuai River basin, China with HEC-HMS model.

PubMed

Gao, Yuqin; Yuan, Yu; Wang, Huaizhi; Schmidt, Arthur R; Wang, Kexuan; Ye, Liu

2017-05-01

The urban agglomeration polders type of flood control pattern is a general flood control pattern in the eastern plain area and some of the secondary river basins in China. A HEC-HMS model of Qinhuai River basin based on the flood control pattern was established for simulating basin runoff, examining the impact of urban agglomeration polders on flood events, and estimating the effects of urbanization on hydrological processes of the urban agglomeration polders in Qinhuai River basin. The results indicate that the urban agglomeration polders could increase the peak flow and flood volume. The smaller the scale of the flood, the more significant the influence of the polder was to the flood volume. The distribution of the city circle polder has no obvious impact on the flood volume, but has effect on the peak flow. The closer the polder is to basin output, the smaller the influence it has on peak flows. As the level of urbanization gradually improving of city circle polder, flood volumes and peak flows gradually increase compared to those with the current level of urbanization (the impervious rate was 20%). The potential change in flood volume and peak flow with increasing impervious rate shows a linear relationship.

Impact of sea level rise on tide gate function.

PubMed

Walsh, Sean; Miskewitz, Robert

2013-01-01

Sea level rise resulting from climate change and land subsidence is expected to severely impact the duration and associated damage resulting from flooding events in tidal communities. These communities must continuously invest resources for the maintenance of existing structures and installation of new flood prevention infrastructure. Tide gates are a common flood prevention structure for low-lying communities in the tidal zone. Tide gates close during incoming tides to prevent inundation from downstream water propagating inland and open during outgoing tides to drain upland areas. Higher downstream mean sea level elevations reduce the effectiveness of tide gates by impacting the hydraulics of the system. This project developed a HEC-RAS and HEC-HMS model of an existing tide gate structure and its upland drainage area in the New Jersey Meadowlands to simulate the impact of rising mean sea level elevations on the tide gate's ability to prevent upstream flooding. Model predictions indicate that sea level rise will reduce the tide gate effectiveness resulting in longer lasting and deeper flood events. The results indicate that there is a critical point in the sea level elevation for this local area, beyond which flooding scenarios become dramatically worse and would have a significantly negative impact on the standard of living and ability to do business in one of the most densely populated areas of America.

Optimal RTP Based Power Scheduling for Residential Load in Smart Grid

NASA Astrophysics Data System (ADS)

Joshi, Hemant I.; Pandya, Vivek J.

2015-12-01

To match supply and demand, shifting of load from peak period to off-peak period is one of the effective solutions. Presently flat rate tariff is used in major part of the world. This type of tariff doesn't give incentives to the customers if they use electrical energy during off-peak period. If real time pricing (RTP) tariff is used, consumers can be encouraged to use energy during off-peak period. Due to advancement in information and communication technology, two-way communications is possible between consumers and utility. To implement this technique in smart grid, home energy controller (HEC), smart meters, home area network (HAN) and communication link between consumers and utility are required. HEC interacts automatically by running an algorithm to find optimal energy consumption schedule for each consumer. However, all the consumers are not allowed to shift their load simultaneously during off-peak period to avoid rebound peak condition. Peak to average ratio (PAR) is considered while carrying out minimization problem. Linear programming problem (LPP) method is used for minimization. The simulation results of this work show the effectiveness of the minimization method adopted. The hardware work is in progress and the program based on the method described here will be made to solve real problem.

Mitochondrial ribosomal protein S18-2 is highly expressed in endometrial cancers along with free E2F1

PubMed Central

Iurchenko, Natalia; Kovalevska, Larysa; Stip, Maria C; Budnikova, Daria; Andersson, Sonia; Polischuk, Ludmila; Buchynska, Lubov; Kashuba, Elena

2016-01-01

Endometrial cancer (EC) is one of the most frequent causes of cancer death among women in developed countries. Histopathological diagnosis and imaging techniques for EC are limited, thus new prognostic markers are needed to offer patients the best treatment and follow-up. In the present paper we showed that the level of mitochondrial ribosomal protein MRPS18-2 (S18-2) increased in EC compared with the normal endometrium and hyperplasia, based on a study of 42 patient biopsies. Importantly, high expression of free E2F1 in EC correlates well with high S18-2 expression. The EC cell line HEC-1-A, which overexpresses S18-2 constitutively, showed an increased proliferation capacity in vitro and in vivo (in SCID mice). Moreover, pan-keratin, beta-catenin and E-cadherin signals are diminished in these cells, compared to the parental HEC-1-A line, in contrast to vimentin signal that is increased. This may be associated with epithelial-mesenchymal cell transition (EMT). We conclude that high expression of S18-2 and free E2F1, and low pan-keratin, beta-catenin, and E-cadherin signals might be a good set of prognostic markers for EC. PMID:26959119

A Regev-Type Fully Homomorphic Encryption Scheme Using Modulus Switching

PubMed Central

Chen, Zhigang; Wang, Jian; Song, Xinxia

2014-01-01

A critical challenge in a fully homomorphic encryption (FHE) scheme is to manage noise. Modulus switching technique is currently the most efficient noise management technique. When using the modulus switching technique to design and implement a FHE scheme, how to choose concrete parameters is an important step, but to our best knowledge, this step has drawn very little attention to the existing FHE researches in the literature. The contributions of this paper are twofold. On one hand, we propose a function of the lower bound of dimension value in the switching techniques depending on the LWE specific security levels. On the other hand, as a case study, we modify the Brakerski FHE scheme (in Crypto 2012) by using the modulus switching technique. We recommend concrete parameter values of our proposed scheme and provide security analysis. Our result shows that the modified FHE scheme is more efficient than the original Brakerski scheme in the same security level. PMID:25093212

Temporal and spatial characteristics of dust devils and their contribution to the aerosol budget in East Asia-An analysis using a new parameterization scheme for dust devils

NASA Astrophysics Data System (ADS)

Tang, Yaoguo; Han, Yongxiang; Liu, Zhaohuan

2018-06-01

Dust aerosols are the main aerosol components of the atmosphere that affect climate change, but the contribution of dust devils to the atmospheric dust aerosol budget is uncertain. In this study, a new parameterization scheme for dust devils was established and coupled with WRF-Chem, and the diurnal and monthly variations and the contribution of dust devils to the atmospheric dust aerosol budget in East Asia was simulated. The results show that 1) both the diurnal and monthly variations in dust devil emissions in East Asia had unimodal distributions, with peaks in the afternoon and the summer that were similar to the observations; 2) the simulated dust devils occurred frequently in deserts, including the Gobi. The distributed area and the intensity center of the dust devil moved from east to west during the day; 3) the ratio between the availability of convective buoyancy relative to the frictional dissipation was the main factor that limited the presence of dust devils. The position of the dust devil formation, the surface temperature, and the boundary layer height determined the dust devil intensity; 4) the contribution of dust devils to atmospheric dust aerosols determined in East Asia was 30.4 ± 13%, thereby suggesting that dust devils contribute significantly to the total amount of atmospheric dust aerosols. Although the new parameterization scheme for dust devils was rough, it was helpful for understanding the distribution of dust devils and their contribution to the dust aerosol budget.

Proton mass decomposition

NASA Astrophysics Data System (ADS)

Yang, Yi-Bo; Chen, Ying; Draper, Terrence; Liang, Jian; Liu, Keh-Fei

2018-03-01

We report the results on the proton mass decomposition and also on the related quark and glue momentum fractions. The results are based on overlap valence fermions on four ensembles of Nf = 2 + 1 DWF configurations with three lattice spacings and volumes, and several pion masses including the physical pion mass. With 1-loop pertur-bative calculation and proper normalization of the glue operator, we find that the u, d, and s quark masses contribute 9(2)% to the proton mass. The quark energy and glue field energy contribute 31(5)% and 37(5)% respectively in the MS scheme at µ = 2 GeV. The trace anomaly gives the remaining 23(1)% contribution. The u, d, s and glue momentum fractions in the MS scheme are consistent with the global analysis at µ = 2 GeV.

Intra-generational Redistribution under Public Pension Planning Based on Generation-based Funding Scheme

NASA Astrophysics Data System (ADS)

Banjo, Daisuke; Tamura, Hiroyuki; Murata, Tadahiko

In this paper, we propose a method of determining the pension in the generation-based funding scheme. In this proposal, we include two types of pensions in the scheme. One is the payment-amount related pension and the other is the payment-frequency related pension. We set the ratio of the total amount of payment-amount related pension to the total amount of both pensions, and simulate income gaps and the relationship between contributions and benefits for each individual when the proposed method is applied.

Sedimentation Impacts Modeling for the Lower Elwha River

NASA Astrophysics Data System (ADS)

Beggs, M.; Kosaka, M.; Sigel, A.; Vandermause, R.; Lauer, J. W.

2012-12-01

The removal of Glines Canyon and Elwha Dams from the Elwha River, northwest Washington, is intended to restore natural geomorphic and ecological processes to the Elwha River basin. Prior to the start of dam removal, over 16 million cubic meters of sediment had accumulated in the reservoirs above the two dams. As dam removal progresses, a portion of this sediment will erode and then be deposited on the downstream river bed and floodplain. To address uncertainty in downstream response to the project, the United States Bureau of Reclamation is implementing an adaptive management plan that relies upon continuous monitoring of water levels at a set of stream gages along the river. To interpret the monitoring data and allow for rapid assessment of the rate of downstream sedimentation, we developed rating curves at several locations along the lower Elwha River. The curves consider a range of possible sedimentation scenarios, each involving different sedimentation levels and/or locations. One scenario considers sedimentation primarily in the river channel, another considers sedimentation primarily on the floodplain, and a third considers both possibilities in tandem. We modeled these scenarios using two separate approaches. First, we modified the cross sections in an existing U.S. Army Corps of Engineers HEC-RAS model to represent possible changes associated with geomorphic adjustment to the dam removals. In-channel sedimentation was assumed to occur as a constant fraction of the bankfull depth at any given section, thereby focusing geomorphic change in relatively deep pool areas. In the HEC-RAS model, off-channel sedimentation was assumed uniform. The HEC-RAS model showed that both low-flow and flood hydraulics are much more sensitive to plausible levels of in-channel sedimentation than to plausible levels of overbank sedimentation. The wide floodplain, complex secondary channels, and geomorphic evolution since the original cross sections were surveyed raise some questions regarding the validity of our 1-D HEC-RAS results and motivated our second approach, which involved developing an independent 2-D hydraulic model using the U.S. Bureau of Reclamation SRH-2d program. This model had the added benefit of being able to utilize more recently surveyed bathymetric and topographic data. The 2-D model was used to improve the representation of spatial variability of likely floodplain sedimentation. For this, we used a preliminary run of the program to characterize the water surface elevation for a typical flood event. We then used the modeled water surface as an input for an eight direction pour point determination of flow direction in ArcGIS. This allowed us to approximate the flow distance from the main channel along streamlines crossing the floodplain. Using observed levee morphology, we developed an ad-hoc exponential function for overbank sedimentation as a function of flow distance from the channel. This tended to focus deposition on natural levees at the upstream side of point bars or meander necks. Despite the more narrowly focused zone of floodplain sedimentation, however, the results were consistent with the 1-D result that in-channel sedimentation is like to have a greater relative impact on system-wide hydraulics than does overbank sedimentation.

Maternal healthcare financing: Gujarat's Chiranjeevi Scheme and its beneficiaries.

PubMed

Bhat, Ramesh; Mavalankar, Dileep V; Singh, Prabal V; Singh, Neelu

2009-04-01

Maternal mortality is an important public-health issue in India, specifically in Gujarat. Contributing factors are the Government's inability to operationalize the First Referral Units and to provide an adequate level of skilled birth attendants, especially to the poor. In response, the Gujarat state has developed a unique public-private partnership called the Chiranjeevi Scheme. This scheme focuses on institutional delivery, specifically emergency obstetric care for the poor. The objective of the study was to explore the targeting of the scheme, its coverage, and socioeconomic profile of the beneficiaries and to assess financial protection offered by the scheme, if any, in Dahod, one of the initial pilot districts of Gujarat. A household-level survey of beneficiaries (n=262) and non-users (n=394) indicated that the scheme is well-targeted to the poor but many poor people do not use the services. The beneficiaries saved more than Rs 3000 (US$ 75) in delivery-related expenses and were generally satisfied with the scheme. The study provided insights on how to improve the scheme further. Such a financing scheme could be replicated in other states and countries to address the cost barrier, especially in areas where high numbers of private specialists are available.

Application of Virtual Rain and Stream Gauge Information Service for Improved Flood Early Warning System in Lower Mekong Countries

NASA Astrophysics Data System (ADS)

Basnayake, S. B.; Jayasinghe, S.; Meechaiya, C.; Markert, K. N.; Lee, H.; Towashiraporn, P.; Anderson, E.; Okeowo, M. A.

2017-12-01

Asia is the most vulnerable region in the world to hydro-meteorological extreme events, exacerbated by climate variability and change. Impacts of floods have been on the rapid increase in the recent decades. Myanmar is one of the most vulnerable countries in the lower Mekong region due to its socioeconomic situation (eg; Nargis in 2008, monsoon floods in 2015, etc). Early warning is an effective way to prepare for hydro-meteorological hazards, to minimize disaster risks; however, early warning systems in Myanmar are seriously hampered by limited observation networks. The Virtual Rain and Stream Gauge Information Service (VRSGIS) has been developed by SERVIR-Mekong program of Asian Disaster Preparedness Center (ADPC) to address these gaps and to provide dense, satellite-based rainfall and water level data, which are calibrated and validated with available in-situ observations. This service would enhance decision making in lower Mekong countries, including Myanmar, to minimize impacts of impending disasters. This service contains rainfall data from GPM IMERG and GSMap, CMORPH, TRMM, and CHIRPS, and water levels for 15 locations using Jason-2/3 altimetry. The virtual daily rainfall data sets are being calibrated with Gamma distribution method and are made publicly accessible through a user-friendly web interface.This paper presents a case study of satellite-derived rainfall data accessed from VRSGIS for hydrological modeling in Myanmar, to estimate inundation areas in Kalay township area of Chindwin River basin during the country's worst flood in 2015. Twelve out of fourteen States of Myanmar were severely affected, 103 people were killed, and one million were displaced due to heavy rains associated with Komen cyclone. The aforementioned rainfall data products are used as inputs for HEC-HMS hydrological runoff model to calculate river flows along Chindwin River, and HEC-RAS hydraulic model is used to estimate inundation areas in downstream including Kalay township area. Model results (inundations) are compared with the estimates of water levels of Jason 2/3 measurements from two locations along the river. The results encourage us to use satellite-derived rainfall data over upstream areas to improve flood modeling, which contributes to improved flood early warning in Myanmar and other lower Mekong countries.

Generation of skeletal mechanism by means of projected entropy participation indices

NASA Astrophysics Data System (ADS)

Paolucci, Samuel; Valorani, Mauro; Ciottoli, Pietro Paolo; Galassi, Riccardo Malpica

2017-11-01

When the dynamics of reactive systems develop very-slow and very-fast time scales separated by a range of active time scales, with gaps in the fast/active and slow/active time scales, then it is possible to achieve multi-scale adaptive model reduction along-with the integration of the ODEs using the G-Scheme. The scheme assumes that the dynamics is decomposed into active, slow, fast, and invariant subspaces. We derive expressions that establish a direct link between time scales and entropy production by using estimates provided by the G-Scheme. To calculate the contribution to entropy production, we resort to a standard model of a constant pressure, adiabatic, batch reactor, where the mixture temperature of the reactants is initially set above the auto-ignition temperature. Numerical experiments show that the contribution to entropy production of the fast subspace is of the same magnitude as the error threshold chosen for the identification of the decomposition of the tangent space, and the contribution of the slow subspace is generally much smaller than that of the active subspace. The information on entropy production associated with reactions within each subspace is used to define an entropy participation index that is subsequently utilized for model reduction.

Supporting public involvement in research design and grant development: a case study of a public involvement award scheme managed by a National Institute for Health Research (NIHR) Research Design Service (RDS).

PubMed

Boote, Jonathan D; Twiddy, Maureen; Baird, Wendy; Birks, Yvonne; Clarke, Clare; Beever, Daniel

2015-10-01

It is good practice for the public to be involved in developing health research. Resources should be available for researchers to fund the involvement of the public in the development of their grants. To describe a funding award scheme to support public involvement in grant development, managed by an NIHR Research Design Service (RDS). Case examples of how the award contributed to successful grant applications and findings from a recent evaluation of the scheme are presented. A case study of resource provision to support public involvement activities in one region of England. University and NHS-based researchers, and members of the public. Between 2009 and 2012, the RDS approved 45 public involvement funding awards (totalling nearly £19,000). These awards contributed to 27 submitted applications at the time of writing, of which 11 were successful (totalling over £7.5 million). The evaluation revealed difficulties encountered by some researchers when involving the public in grant development, which led to suggestions about how the scheme could be improved. This award scheme represents an efficient method of providing researchers with resources to involve the public in grant development and would appear to represent good value for money. © 2013 John Wiley & Sons Ltd.

Pakistan Journal of Medical Sciences: A bibliometric assessment 2001-2010.

PubMed

Baladi, Zameer Hussain; Umedani, Loung V

2017-01-01

The aim of this study was to measure the growth of scientific research, authors' productivity, affiliation with the institute and geographic locations published in the Pakistan Journal of Medical Sciences during the period of 2001 - 2010. This numerical analysis was conducted during mid-August 2016 to mid-October, 2016. The data for the study was downloaded from websites of e-journal of Pakistan Journal of Medical Sciences (PJMS) and Pak Medi-Net Com. A total number of 1199 articled were covered by PJMS in 10 volumes and 40 issues with contribution of 3798 (3%) authors during 2001 - 2010. The average number of papers per issue is 30%. A gender wise contribution of males was higher 3050 (80%) than the females 748 (20%). A majority of articles were multi-authored 1052 (87%) as opposed to single author contribution 147 (13%). All 1199 articles were covered under four major disciplines i.e Basic medical sciences, medicine & allied, surgery & allied and radiological sciences and 39 sub-specialties according to medical subject headings (MeSH). It observed that 467 (39%) articles were published in Pakistan and 732 (61%) articles produced by other 32 countries. The Karachi city of Pakistan has produced 199 (16%) articles as highest as its national level and followed by Tehran (Iran) 77 (6%) as followed internationally. This study reveals that the participation of 32 countries in the PJMS publications proves it to be an internationally circulated journal to support research with the constant approach of publishing articles to each volume in basic medical sciences, biomedical, clinical and public health sciences. Abbreviations: DOAJ: Directory of Open Access Journals IMEMR: Index Medicus Eastern Mediterranean Region HEC: Higher Education Commission (Pakistan) PJMS: Pakistan Journal of Medical Sciences MeSH: Medical Subject Headings PMDC: Pakistan Medical & Dental Council SCIE: Science Citation Index Expanded.

Estimating the proportion of groundwater recharge from flood events in relation to total annual recharge in a karst aquifer

NASA Astrophysics Data System (ADS)

Dvory, N. Z.; Ronen, A.; Livshitz, Y.; Adar, E.; Kuznetsov, M.; Yakirevich, A.

2017-12-01

Sustainable groundwater production from karstic aquifers is primarily dictated by its recharge rate. Therefore, in order to limit over-exploitation, it is essential to accurately quantify groundwater recharge. Infiltration during erratic floods in karstic basins may contribute substantial amount to aquifer recharge. However, the complicated nature of karst systems, which are characterized in part by multiple springs, sinkholes, and losing/gaining streams, present a large obstacle to accurately assess the actual contribution of flood water to groundwater recharge. In this study, we aim to quantify the proportion of groundwater recharge during flood events in relation to the annual recharge for karst aquifers. The role of karst conduits on flash flood infiltration was examined during four flood and artificial runoff events in the Sorek creek near Jerusalem, Israel. The events were monitored in short time steps (four minutes). This high resolution analysis is essential to accurately estimating surface flow volumes, which are of particular importance in arid and semi-arid climate where ephemeral flows may provide a substantial contribution to the groundwater reservoirs. For the present investigation, we distinguished between direct infiltration, percolation through karst conduits and diffused infiltration, which is most affected by evapotranspiration. A water balance was then calculated for the 2014/15 hydrologic year using the Hydrologic Engineering Center - Hydrologic Modelling System (HEC-HMS). Simulations show that an additional 8% to 24% of the annual recharge volume is added from runoff losses along the creek that infiltrate through the karst system into the aquifer. The results improve the understanding of recharge processes and support the use of the proposed methodology for quantifying groundwater recharge.

A Simulation of an Income Contingent Tuition Scheme in a Transition Economy

ERIC Educational Resources Information Center

Vodopivec, Milan

2009-01-01

The paper takes advantage of exceptionally rich longitudinal data on the universe of labor force participants in Slovenia and simulates the working of an income contingent loan scheme that seeks to recover part of schooling costs. The simulations show that under the base variant (where the target cost recovery rate is 20% and the contribution rate…

VA/DoD Joint Executive Council FY 2009: Joint Strategic Plan FY 2010-2012

DTIC Science & Technology

2009-01-01

through the Military Health System (MHS) Population Health Portal . HEC Traumatic Brain Injury and Psychological Health In FY 2009, VA and DoD made...available using the Deployment Occupational and Environmental Health Surveillance Portal . TBI and MH assessment tools were evaluated and monitored through...with increased access, VA maintained the frequency of encounters for treatment of PTSD and other MH conditions in Veterans of prior eras. To forecast

Health Care Coverage Decision Making in Low- and Middle-Income Countries: Experiences from 25 Coverage Schemes.

PubMed

Gutierrez, Hialy; Shewade, Ashwini; Dai, Minghan; Mendoza-Arana, Pedro; Gómez-Dantés, Octavio; Jain, Nishant; Khonelidze, Irma; Nabyonga-Orem, Juliet; Saleh, Karima; Teerawattananon, Yot; Nishtar, Sania; Hornberger, John

2015-08-01

Lessons learned by countries that have successfully implemented coverage schemes for health services may be valuable for other countries, especially low- and middle-income countries (LMICs), which likewise are seeking to provide/expand coverage. The research team surveyed experts in population health management from LMICs for information on characteristics of health care coverage schemes and factors that influenced decision-making processes. The level of coverage provided by the different schemes varied. Nearly all the health care coverage schemes involved various representatives and stakeholders in their decision-making processes. Maternal and child health, cardiovascular diseases, cancer, and HIV were among the highest priorities guiding coverage development decisions. Evidence used to inform coverage decisions included medical literature, regional and global epidemiology, and coverage policies of other coverage schemes. Funding was the most commonly reported reason for restricting coverage. This exploratory study provides an overview of health care coverage schemes from participating LMICs and contributes to the scarce evidence base on coverage decision making. Sharing knowledge and experiences among LMICs can support efforts to establish systems for accessible, affordable, and equitable health care.

A no-fault compensation system for medical injury is long overdue.

PubMed

Weisbrot, David; Breen, Kerry J

2012-09-03

The 2011 report of the Productivity Commission (PC) recommended the establishment of a no-fault national injury insurance scheme limited to "catastrophic" injury, including medical injury. The report is welcome, but represents a missed opportunity to establish simultaneously a much-needed no-fault scheme for all medical injuries. The existing indemnity scheme based on negligence remains a slow, costly, inefficient, ill targeted and stress-creating system. A fault-based negligence scheme cannot deter non-intentional errors and does little to identify or prevent systems failures. In addition, it discourages reporting, and thus is antithetical to the modern focus on universal patient safety. A no-fault scheme has the potential to be fairer, quicker and no more costly, and to contribute to patient safety. No-fault schemes have been in place in at least six developed countries for many years. This extensive experience in comparable countries should be examined to assist Australia to design an effective, comprehensive system. Before implementing the recommendations of the PC, the federal government should ask the Commission to study and promptly report on an ancillary no-fault scheme that covers all medical injury.

A Scheme to Smooth Aggregated Traffic from Sensors with Periodic Reports

PubMed Central

Oh, Sungmin; Jang, Ju Wook

2017-01-01

The possibility of smoothing aggregated traffic from sensors with varying reporting periods and frame sizes to be carried on an access link is investigated. A straightforward optimization would take O(pn) time, whereas our heuristic scheme takes O(np) time where n, p denote the number of sensors and size of periods, respectively. Our heuristic scheme performs local optimization sensor by sensor, starting with the smallest to largest periods. This is based on an observation that sensors with large offsets have more choices in offsets to avoid traffic peaks than the sensors with smaller periods. A MATLAB simulation shows that our scheme excels the known scheme by M. Grenier et al. in a similar situation (aggregating periodic traffic in a controller area network) for almost all possible permutations. The performance of our scheme is very close to the straightforward optimization, which compares all possible permutations. We expect that our scheme would greatly contribute in smoothing the traffic from an ever-increasing number of IoT sensors to the gateway, reducing the burden on the access link to the Internet. PMID:28273831

A Scheme to Smooth Aggregated Traffic from Sensors with Periodic Reports.

PubMed

Oh, Sungmin; Jang, Ju Wook

2017-03-03

The possibility of smoothing aggregated traffic from sensors with varying reporting periods and frame sizes to be carried on an access link is investigated. A straightforward optimization would take O(pn) time, whereas our heuristic scheme takes O(np) time where n, p denote the number of sensors and size of periods, respectively. Our heuristic scheme performs local optimization sensor by sensor, starting with the smallest to largest periods. This is based on an observation that sensors with large offsets have more choices in offsets to avoid traffic peaks than the sensors with smaller periods. A MATLAB simulation shows that our scheme excels the known scheme by M. Grenier et al. in a similar situation (aggregating periodic traffic in a controller area network) for almost all possible permutations. The performance of our scheme is very close to the straightforward optimization, which compares all possible permutations. We expect that our scheme would greatly contribute in smoothing the traffic from an ever-increasing number of IoT sensors to the gateway, reducing the burden on the access link to the Internet.

Mirror Birefringence in a Fabry-Perot Cavity and the Detection of Vacuum Birefringence in a Magnetic Field

NASA Technical Reports Server (NTRS)

Chui, T. C. P.; Shao, M.; Redding, D.; Gursel, Y.; Boden, A.

1995-01-01

We discuss the effect of mirror birefringence in two optical schemes designed to detect the quantum-electrodynamics (QED) predictions of vacuum birefringence under the influence of a strong magnetic field, B. Both schemes make use of a high finesse Fabry-Perot cavity (F-P) to increase the average path length of the light in the magnetic field. The first scheme, which we called the frequency scheme, is based on measurement of the beat frequency of two orthogonal polarized laser beams in the cavity. We show that mirror birefringence contributes to the detection uncertainties in first order, resulting in a high susceptibility to small thermal disturbances. We estimate that an unreasonably high thermal stability of 10-9 K is required to resolve the effect to 0.1%. In the second scheme, which we called the polarization rotation scheme, laser polarized at 45 relative to the B field is injected into the cavity.

Design of an extensive information representation scheme for clinical narratives.

PubMed

Deléger, Louise; Campillos, Leonardo; Ligozat, Anne-Laure; Névéol, Aurélie

2017-09-11

Knowledge representation frameworks are essential to the understanding of complex biomedical processes, and to the analysis of biomedical texts that describe them. Combined with natural language processing (NLP), they have the potential to contribute to retrospective studies by unlocking important phenotyping information contained in the narrative content of electronic health records (EHRs). This work aims to develop an extensive information representation scheme for clinical information contained in EHR narratives, and to support secondary use of EHR narrative data to answer clinical questions. We review recent work that proposed information representation schemes and applied them to the analysis of clinical narratives. We then propose a unifying scheme that supports the extraction of information to address a large variety of clinical questions. We devised a new information representation scheme for clinical narratives that comprises 13 entities, 11 attributes and 37 relations. The associated annotation guidelines can be used to consistently apply the scheme to clinical narratives and are https://cabernet.limsi.fr/annotation_guide_for_the_merlot_french_clinical_corpus-Sept2016.pdf . The information scheme includes many elements of the major schemes described in the clinical natural language processing literature, as well as a uniquely detailed set of relations.

Vanpool trip planning based on evolutionary multiple objective optimization

NASA Astrophysics Data System (ADS)

Zhao, Ming; Yang, Disheng; Feng, Shibing; Liu, Hengchang

2017-08-01

Carpool and vanpool draw a lot of researchers’ attention, which is the emphasis of this paper. A concrete vanpool operation definition is given, based on the given definition, this paper tackles vanpool operation optimization using user experience decline index(UEDI). This paper is focused on making each user having identical UEDI and the system having minimum sum of all users’ UEDI. Three contributions are made, the first contribution is a vanpool operation scheme diagram, each component of the scheme is explained in detail. The second contribution is getting all customer’s UEDI as a set, standard deviation and sum of all users’ UEDI set are used as objectives in multiple objective optimization to decide trip start address, trip start time and trip destination address. The third contribution is a trip planning algorithm, which tries to minimize the sum of all users’ UEDI. Geographical distribution of the charging stations and utilization rate of the charging stations are considered in the trip planning process.

Modelling winter organic aerosol at the European scale with CAMx: evaluation and source apportionment with a VBS parameterization based on novel wood burning smog chamber experiments

NASA Astrophysics Data System (ADS)

Ciarelli, Giancarlo; Aksoyoglu, Sebnem; El Haddad, Imad; Bruns, Emily A.; Crippa, Monica; Poulain, Laurent; Äijälä, Mikko; Carbone, Samara; Freney, Evelyn; O'Dowd, Colin; Baltensperger, Urs; Prévôt, André S. H.

2017-06-01

We evaluated a modified VBS (volatility basis set) scheme to treat biomass-burning-like organic aerosol (BBOA) implemented in CAMx (Comprehensive Air Quality Model with extensions). The updated scheme was parameterized with novel wood combustion smog chamber experiments using a hybrid VBS framework which accounts for a mixture of wood burning organic aerosol precursors and their further functionalization and fragmentation in the atmosphere. The new scheme was evaluated for one of the winter EMEP intensive campaigns (February-March 2009) against aerosol mass spectrometer (AMS) measurements performed at 11 sites in Europe. We found a considerable improvement for the modelled organic aerosol (OA) mass compared to our previous model application with the mean fractional bias (MFB) reduced from -61 to -29 %. We performed model-based source apportionment studies and compared results against positive matrix factorization (PMF) analysis performed on OA AMS data. Both model and observations suggest that OA was mainly of secondary origin at almost all sites. Modelled secondary organic aerosol (SOA) contributions to total OA varied from 32 to 88 % (with an average contribution of 62 %) and absolute concentrations were generally under-predicted. Modelled primary hydrocarbon-like organic aerosol (HOA) and primary biomass-burning-like aerosol (BBPOA) fractions contributed to a lesser extent (HOA from 3 to 30 %, and BBPOA from 1 to 39 %) with average contributions of 13 and 25 %, respectively. Modelled BBPOA fractions were found to represent 12 to 64 % of the total residential-heating-related OA, with increasing contributions at stations located in the northern part of the domain. Source apportionment studies were performed to assess the contribution of residential and non-residential combustion precursors to the total SOA. Non-residential combustion and road transportation sector contributed about 30-40 % to SOA formation (with increasing contributions at urban and near industrialized sites), whereas residential combustion (mainly related to wood burning) contributed to a larger extent, around 60-70 %. Contributions to OA from residential combustion precursors in different volatility ranges were also assessed: our results indicate that residential combustion gas-phase precursors in the semivolatile range (SVOC) contributed from 6 to 30 %, with higher contributions predicted at stations located in the southern part of the domain. On the other hand, the oxidation products of higher-volatility precursors (the sum of intermediate-volatility compounds (IVOCs) and volatile organic compounds (VOCs)) contribute from 15 to 38 % with no specific gradient among the stations. Although the new parameterization leads to a better agreement between model results and observations, it still under-predicts the SOA fraction, suggesting that uncertainties in the new scheme and other sources and/or formation mechanisms remain to be elucidated. Moreover, a more detailed characterization of the semivolatile components of the emissions is needed.

Numerical Limitations of 1D Hydraulic Models Using MIKE11 or HEC-RAS software - Case study of Baraolt River, Romania

NASA Astrophysics Data System (ADS)

Andrei, Armas; Robert, Beilicci; Erika, Beilicci

2017-10-01

MIKE 11 is an advanced hydroinformatic tool, a professional engineering software package for simulation of one-dimensional flows in estuaries, rivers, irrigation systems, channels and other water bodies. MIKE 11 is a 1-dimensional river model. It was developed by DHI Water · Environment · Health, Denmark. The basic computational procedure of HEC-RAS for steady flow is based on the solution of the one-dimensional energy equation. Energy losses are evaluated by friction and contraction / expansion. The momentum equation may be used in situations where the water surface profile is rapidly varied. These situations include hydraulic jumps, hydraulics of bridges, and evaluating profiles at river confluences. For unsteady flow, HEC-RAS solves the full, dynamic, 1-D Saint Venant Equation using an implicit, finite difference method. The unsteady flow equation solver was adapted from Dr. Robert L. Barkau’s UNET package. Fluid motion is controlled by the basic principles of conservation of mass, energy and momentum, which form the basis of fluid mechanics and hydraulic engineering. Complex flow situations must be solved using empirical approximations and numerical models, which are based on derivations of the basic principles (backwater equation, Navier-Stokes equation etc.). All numerical models are required to make some form of approximation to solve these principles, and consequently all have their limitations. The study of hydraulics and fluid mechanics is founded on the three basic principles of conservation of mass, energy and momentum. Real-life situations are frequently too complex to solve without the aid of numerical models. There is a tendency among some engineers to discard the basic principles taught at university and blindly assume that the results produced by the model are correct. Regardless of the complexity of models and despite the claims of their developers, all numerical models are required to make approximations. These may be related to geometric limitations, numerical simplification, or the use of empirical correlations. Some are obvious: one-dimensional models must average properties over the two remaining directions. It is the less obvious and poorly advertised approximations that pose the greatest threat to the novice user. Some of these, such as the inability of one-dimensional unsteady models to simulate supercritical flow can cause significant inaccuracy in the model predictions.

Combining abiotic and biotic models - Hydraulical modeling to fill the gap between catchment and hydro-dynamic models

NASA Astrophysics Data System (ADS)

Guse, B.; Sulc, D.; Schmalz, B.; Fohrer, N.

2012-04-01

The European Water Framework Directive (WFD) requires a catchment-based approach, which is assessed in the IMPACT project by combining abiotic and biotic models. The core point of IMPACT is a model chain (catchment model -> 1-D-hydraulic model -> 3-D-hydro-morphodynamic model -> biotic habitat model) with the aim to estimate the occurrence of the target species of the WFD. Firstly, the model chain is developed for the current land use and climate conditions. Secondly, land use and climate change scenarios are developed at the catchment scale. The outputs of the catchment model for the scenarios are used as input for the next models within the model chain to estimate the effect of these changes on the target species. The eco-hydrological catchment model SWAT is applied for the Treene catchment in Northern Germany and delivers discharge and water quality parameters as a spatial explicit output for each subbasin. There is no water level information given by SWAT. However, water level values are needed as lower boundary condition for the hydro-dynamic and habitat models which are applied for the 300 m candidate reference reach. In order to fill the gap between the catchment and the hydro-morphodynamic model, the 1-D hydraulic model HEC-RAS is applied for a 3 km long reach transect from the next upstream hydrological station until the upper bound of the candidate study reach. The channel geometry for HEC-RAS was estimated based on 96 cross-sections which were measured in the IMPACT project. By using available discharge and water level measurements from the hydrological station and own flow velocity measurements, the channel resistence was estimated. HEC-RAS was run with different statistical indices (mean annual drought, mean discharge, …) for steady flow conditions. The rating curve was then constructed for the target cross-section, i.e. the lower bound of the candidate study reach, to fulfill the combining with the hydro- and morphodynamic models. These statistical indices can also be calculated for the discharge series provided by land use and climate scenarios. In this way, the effect of land use and climate change on the catchment and the hydraulic processes can be assessed.

New auto-tuning technique for the hydrogen maser

NASA Technical Reports Server (NTRS)

Sydnor, R. L.; Maleki, L.

1983-01-01

Auto-tuning of the maser cavity compensates for cavity pulling effect, and other sources of contribution to the long term frequency drift. Schemes previously proposed for the maser cavity auto-tuning can have adverse effects on the performance of the maser. A new scheme is proposed based on the phase relationship between the electric and the magnetic fields inside the cavity. This technique has the desired feature of auto-tuning the cavity with a very high sensitivity and without disturbing the maser performance. Some approaches for the implementation of this scheme and possible areas of difficulty are examined.

The analytical {\\mathscr{O}}({a}_{s}^{4}) expression for the polarized Bjorken sum rule in the miniMOM scheme and the consequences for the generalized Crewther relation

NASA Astrophysics Data System (ADS)

Kataev, A. L.; Molokoedov, V. S.

2017-12-01

The analytical {\\mathscr{O}}({a}s4) perturbative QCD expression for the flavour non-singlet contribution to the Bjorken polarized sum rule in the rather applicable at present gauge-dependent miniMOM scheme is obtained. For the considered three values of the gauge parameter, namely ξ = 0 (Landau gauge), ξ = -1 (anti-Feynman gauge) and ξ = -3 (Stefanis-Mikhailov gauge), the scheme-dependent coefficients are considerably smaller than the gauge-independent {\\overline{{MS}}} results. It is found that the fundamental property of the factorization of the QCD renormalization group β-function in the generalized Crewther relation, which is valid in the gauge-invariant {\\overline{{MS}}} scheme up to {\\mathscr{O}}({a}s4)-level at least, is unexpectedly valid at the same level in the miniMOM-scheme for ξ = 0, and for ξ = -1 and ξ = -3 in part.

Hybrid fiber links for accurate optical frequency comparison

NASA Astrophysics Data System (ADS)

Lee, Won-Kyu; Stefani, Fabio; Bercy, Anthony; Lopez, Olivier; Amy-Klein, Anne; Pottie, Paul-Eric

2017-05-01

We present the experimental demonstration of a local two-way optical frequency comparison over a 43-km-long urban fiber network without any requirement for measurement synchronization. We combined the local two-way scheme with a regular active noise compensation scheme that was implemented on another parallel fiber leading to a highly reliable and robust frequency transfer. This hybrid scheme allowed us to investigate the major limiting factors of the local two-way comparison. We analyzed the contributions of the interferometers at both local and remote locations to the phase noise of the local two-way signal. Using the ability of this setup to be injected by either a single laser or two independent lasers, we measured the contributions of the demodulated laser instabilities to the long-term instability. We show that a fractional frequency instability level of 10-20 at 10,000 s can be obtained using this simple setup after propagation over a distance of 43 km in an urban area.

Methodology for predicting maximum velocity and shear stress in a sinuous channel with bendway weirs using 1-D HEC-RAS modeling results

Treesearch

Paul Sclafani

2011-01-01

The Middle Rio Grande is a 29-mi reach of the Rio Grande River in central New Mexico that extends from downstream of Cochiti Dam to Bernalillo, New Mexico. A series of anthropogenic factors including the construction of flood control levees and Cochiti Dam have altered the historically-braided morphology of the Middle Rio Grande to a more sinuous, degrading reach, with...

Design of Explosion Blast Containment Vessels for Explosive Ordnance Disposal Units

DTIC Science & Technology

1975-06-01

gages installed on Vessels 1 and 3 were Type EP-08-250BF-350. ,hec: gages, which are special , annealed constantan foil, have a quoted strain range of...either 32 or 24 Vdc from automotive-type wet cell batteries, although large dry cells may be used with slightly reduced stability. The bridge output is...RST-CYCLE VESEL RESPONSE I. -- ~-~- --. - - ,--~ -- --- -~ - - -- ~ ~ -- 72 TABLE 10. DESCRIPTION OF BLACK POWDER SHOTS FIRED IN 3.0-FT VESSEL Shot

Impact of different parameterization schemes on simulation of mesoscale convective system over south-east India

NASA Astrophysics Data System (ADS)

Madhulatha, A.; Rajeevan, M.

2018-02-01

Main objective of the present paper is to examine the role of various parameterization schemes in simulating the evolution of mesoscale convective system (MCS) occurred over south-east India. Using the Weather Research and Forecasting (WRF) model, numerical experiments are conducted by considering various planetary boundary layer, microphysics, and cumulus parameterization schemes. Performances of different schemes are evaluated by examining boundary layer, reflectivity, and precipitation features of MCS using ground-based and satellite observations. Among various physical parameterization schemes, Mellor-Yamada-Janjic (MYJ) boundary layer scheme is able to produce deep boundary layer height by simulating warm temperatures necessary for storm initiation; Thompson (THM) microphysics scheme is capable to simulate the reflectivity by reasonable distribution of different hydrometeors during various stages of system; Betts-Miller-Janjic (BMJ) cumulus scheme is able to capture the precipitation by proper representation of convective instability associated with MCS. Present analysis suggests that MYJ, a local turbulent kinetic energy boundary layer scheme, which accounts strong vertical mixing; THM, a six-class hybrid moment microphysics scheme, which considers number concentration along with mixing ratio of rain hydrometeors; and BMJ, a closure cumulus scheme, which adjusts thermodynamic profiles based on climatological profiles might have contributed for better performance of respective model simulations. Numerical simulation carried out using the above combination of schemes is able to capture storm initiation, propagation, surface variations, thermodynamic structure, and precipitation features reasonably well. This study clearly demonstrates that the simulation of MCS characteristics is highly sensitive to the choice of parameterization schemes.

Emodin enhances the chemosensitivity of endometrial cancer by inhibiting ROS-mediated Cisplatin-resistance.

PubMed

Ding, Ning; Zhang, Hong; Su, Shan; Ding, Yumei; Yu, Xiaohui; Tang, Yujie; Wang, Qingfang; Liu, Peishu

2017-12-18

Background Endometrial cancer is a common cause of death in gynecological malignancies. Cisplatin is a clinically chemotherapeutic agent. However, drug-resistance is the primary cause of treatment failure. Objective Emodin is commonly used clinically to increase the sensitivity of chemotherapeutic agents, yet whether Emodin promotes the role of Cisplatin in the treatment of endometrial cancer has not been studied. Method CCK-8 kit was utilized to determine the growth of two endometrial cancer cell lines, Ishikawa and HEC-IB. The apoptosis level of Ishikawa and HEC-IB cells was detected by Annexin V / propidium iodide double-staining assay. ROS level was detected by DCFH-DA and NADPH oxidase expression. Expressions of drug-resistant genes were examined by real-time PCR and Western blotting. Results Emodin combined with Cisplatin reduced cell growth and increased the apoptosis of endometrial cancer cells. Co-treatment of Emodin and Cisplatin increased chemosensitivity by inhibiting the expression of drug-resistant genes through reducing the ROS levels in endometrial cancer cells. In an endometrial cancer xenograft murine model, the tumor size was reduced and animal survival time was increased by co-treatment of Emodin and Cisplatin. Conclusion This study demonstrates that Emodin enhances the chemosensitivity of Cisplatin on endometrial cancer by inhibiting ROS-mediated expression of drug-resistance genes. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

Comparison of Chlamydia trachomatis serovar L2 growth in polarized genital epithelial cells grown in three-dimensional culture with non-polarized cells.

PubMed

Dessus-Babus, Sophie; Moore, Cheryl G; Whittimore, Judy D; Wyrick, Priscilla B

2008-04-01

A common model for studying Chlamydia trachomatis and growing chlamydial stocks uses Lymphogranuloma venereum serovar L2 and non-polarized HeLa cells. However, recent publications indicate that the growth rate and progeny yields can vary considerably for a particular strain depending on the cell line/type used, and seem to be partially related to cell tropism. In the present study, the growth of invasive serovar L2 was compared in endometrial HEC-1B and endocervical HeLa cells polarized on collagen-coated microcarrier beads, as well as in HeLa cells grown in tissue culture flasks. Microscopy analysis revealed no difference in chlamydial attachment/entry patterns or in inclusion development throughout the developmental cycle between cell lines. Very comparable growth curves in both cell lines were also found using real-time PCR analysis, with increases in chlamydial DNA content of 400-500-fold between 2 and 36 h post-inoculation. Similar progeny yields with comparable infectivity were recovered from HEC-1B and HeLa cell bead cultures, and no difference in chlamydial growth was found in polarized vs. non-polarized HeLa cells. In conclusion, unlike other C. trachomatis strains such as urogenital serovar E, invasive serovar L2 grows equally well in physiologically different endometrial and endocervical environments, regardless of the host cell polarization state.

G Protein-Coupled Estrogen Receptor-Selective Ligands Modulate Endometrial Tumor Growth

PubMed Central

Petrie, Whitney K.; Dennis, Megan K.; Dai, Donghai; Arterburn, Jeffrey B.; Smith, Harriet O.; Hathaway, Helen J.; Prossnitz, Eric R.

2013-01-01

Endometrial carcinoma is the most common cancer of the female reproductive tract. GPER/GPR30 is a 7-transmembrane spanning G protein-coupled receptor that has been identified as the third estrogen receptor, in addition to ERα and ERβ. High GPER expression is predictive of poor survival in endometrial and ovarian cancer, but despite this, the estrogen-mediated signaling pathways and specific estrogen receptors involved in endometrial cancer remain unclear. Here, employing ERα-negative Hec50 endometrial cancer cells, we demonstrate that GPER mediates estrogen-stimulated activation of ERK and PI3K via matrix metalloproteinase activation and subsequent transactivation of the EGFR and that ER-targeted therapeutic agents (4-hydroxytamoxifen, ICI182,780/fulvestrant, and Raloxifene), the phytoestrogen genistein, and the “ERα-selective” agonist propylpyrazole triol also function as GPER agonists. Furthermore, xenograft tumors of Hec50 cells yield enhanced growth with G-1 and estrogen, the latter being inhibited by GPER-selective pharmacologic antagonism with G36. These results have important implications with respect to the use of putatively ER-selective ligands and particularly for the widespread long-term use of “ER-targeted” therapeutics. Moreover, our findings shed light on the potential mechanisms of SERM/SERD side effects reported in many clinical studies. Finally, our results provide the first demonstration that pharmacological inhibition of GPER activity in vivo prevents estrogen-mediated tumor growth. PMID:24379833

Developing an Approach to Prioritize River Restoration using Data Extracted from Flood Risk Information System Databases.

NASA Astrophysics Data System (ADS)

Vimal, S.; Tarboton, D. G.; Band, L. E.; Duncan, J. M.; Lovette, J. P.; Corzo, G.; Miles, B.

2015-12-01

Prioritizing river restoration requires information on river geometry. In many states in the US detailed river geometry has been collected for floodplain mapping and is available in Flood Risk Information Systems (FRIS). In particular, North Carolina has, for its 100 Counties, developed a database of numerous HEC-RAS models which are available through its Flood Risk Information System (FRIS). These models that include over 260 variables were developed and updated by numerous contractors. They contain detailed surveyed or LiDAR derived cross-sections and modeled flood extents for different extreme event return periods. In this work, over 4700 HEC-RAS models' data was integrated and upscaled to utilize detailed cross-section information and 100-year modelled flood extent information to enable river restoration prioritization for the entire state of North Carolina. We developed procedures to extract geomorphic properties such as entrenchment ratio, incision ratio, etc. from these models. Entrenchment ratio quantifies the vertical containment of rivers and thereby their vulnerability to flooding and incision ratio quantifies the depth per unit width. A map of entrenchment ratio for the whole state was derived by linking these model results to a geodatabase. A ranking of highly entrenched counties enabling prioritization for flood allowance and mitigation was obtained. The results were shared through HydroShare and web maps developed for their visualization using Google Maps Engine API.

G protein-coupled estrogen receptor-selective ligands modulate endometrial tumor growth.

PubMed

Petrie, Whitney K; Dennis, Megan K; Hu, Chelin; Dai, Donghai; Arterburn, Jeffrey B; Smith, Harriet O; Hathaway, Helen J; Prossnitz, Eric R

2013-01-01

Endometrial carcinoma is the most common cancer of the female reproductive tract. GPER/GPR30 is a 7-transmembrane spanning G protein-coupled receptor that has been identified as the third estrogen receptor, in addition to ERα and ERβ. High GPER expression is predictive of poor survival in endometrial and ovarian cancer, but despite this, the estrogen-mediated signaling pathways and specific estrogen receptors involved in endometrial cancer remain unclear. Here, employing ERα-negative Hec50 endometrial cancer cells, we demonstrate that GPER mediates estrogen-stimulated activation of ERK and PI3K via matrix metalloproteinase activation and subsequent transactivation of the EGFR and that ER-targeted therapeutic agents (4-hydroxytamoxifen, ICI182,780/fulvestrant, and Raloxifene), the phytoestrogen genistein, and the "ERα-selective" agonist propylpyrazole triol also function as GPER agonists. Furthermore, xenograft tumors of Hec50 cells yield enhanced growth with G-1 and estrogen, the latter being inhibited by GPER-selective pharmacologic antagonism with G36. These results have important implications with respect to the use of putatively ER-selective ligands and particularly for the widespread long-term use of "ER-targeted" therapeutics. Moreover, our findings shed light on the potential mechanisms of SERM/SERD side effects reported in many clinical studies. Finally, our results provide the first demonstration that pharmacological inhibition of GPER activity in vivo prevents estrogen-mediated tumor growth.

Preclinical pilot study monitoring topical drug penetration and dermal bioavailability of a peptidase inhibitor from different galenic formulations into pig dermis, using cutaneous microdialysis.

PubMed

Quist, S R; Heimburg, A; Bank, U; Mahnkopf, D; Koch, G; Gollnick, H; Täger, M; Ansorge, S

2017-08-01

Cutaneous microdialysis (CM) is an ex vivo technique that allows study of tissue chemistry, including bioavailability of actual tissue concentration of unbound drug in the interstitial fluid of the body. To test the penetration and dermal bioavailability of galenic formulations of the small-molecule IP10.C8, a dual-protease inhibitor of the dipeptidyl peptidase and aminopeptidase families. Using CM, we tested the penetration and dermal bioavailability of IP10.C8 into the dermis and subcutis of pigs, and determined the tissue concentration of IP10.C8 enzymatically, using an enzyme activity assay (substrate Gly-Pro-pNA) and high performance liquid chromatography. Dermal bioavailability was enhanced by using microemulsion or the addition of the penetration enhancer oleic acid to a hydroxyethylcellulose (HEC) gel formulation. Dermal bioavailability was also enhanced when galenic formulations were prepared with higher pH (7.5 vs. 6.5) or higher drug concentration (5% vs. 1%) in HEC gel. It seems possible, using CM for topical skin penetration testing in anaesthetized domestic pigs, to test the bioavailability of newly designed drugs. However, the experimental time is limited due to the anaesthesia, and is dependent on drug recovery. Validation of this technique for routine use is challenging, and more experiments are needed to validate this preclinical set-up. © 2017 British Association of Dermatologists.

Study of the influence of platinum, palladium and rhodium on duckweed (Lemna minor).

PubMed

Bednarova, Ivana; Mikulaskova, Hana; Havelkova, Barbora; Strakova, Lenka; Beklova, Miroslava; Sochor, Jiri; Hynek, David; Adam, Vojtech; Kizek, Rene

2014-01-01

Road traffic pollutants and the residues of cytostatics that are widely used in anti-cancer therapy are a significant sources of platinum group elements (PGE; Pt, Pd and Rh) in environment. These metals can migrate into sewage and thus pollute surface waters. The purpose of our study was to evaluate the effect of PtCl4 on the antioxidant and enzymatic activity of duckweed (Lemna minor), a bioindicator of the aquatic environment. The study was performed using a 7-day conventional test based on the OECD 221 (CSN EN ISO 20079)--Lemna sp. Growth Inhibition Test. We also conducted a microbiotest to analyse the effects of PtC4, PdCl2 and RhCl3 on the morphology and vegetative growth of colonies of this plant and compared their inhibitory effects during the microbiotest. We observed inhibition of colony growth and clear morphological changes. Antioxidant and enzymatic activities increased with platinum doses increased. The 168hEC50 of PtCl4 was 12.16 μM (95% confidence interval = 9.88-14.44) and the 168hEC50 of PdCl2 was 50.39 (95% confidence interval = 23.83-76.96). The greatest inhibition of growth by RhCl3 was observed at 25 μM. The obtained results suggest that L. minor phytotoxicity tests should be widely used in the biomonitoring.

Barriers and facilitators to implementation, uptake and sustainability of community-based health insurance schemes in low- and middle-income countries: a systematic review.

PubMed

Fadlallah, Racha; El-Jardali, Fadi; Hemadi, Nour; Morsi, Rami Z; Abou Samra, Clara Abou; Ahmad, Ali; Arif, Khurram; Hishi, Lama; Honein-AbouHaidar, Gladys; Akl, Elie A

2018-01-29

Community-based health insurance (CBHI) has evolved as an alternative health financing mechanism to out of pocket payments in low- and middle-income countries (LMICs), particularly in areas where government or employer-based health insurance is minimal. This systematic review aimed to assess the barriers and facilitators to implementation, uptake and sustainability of CHBI schemes in LMICs. We searched six electronic databases and grey literature. We included both quantitative and qualitative studies written in English language and published after year 1992. Two reviewers worked in duplicate and independently to complete study selection, data abstraction, and assessment of methodological features. We synthesized the findings based on thematic analysis and categorized according to the ecological model into individual, interpersonal, community and systems levels. Of 15,510 citations, 51 met the eligibility criteria. Individual factors included awareness and understanding of the concept of CBHI, trust in scheme and scheme managers, perceived service quality, and demographic characteristics, which influenced enrollment and sustainability. Interpersonal factors such as household dynamics, other family members enrolled in the scheme, and social solidarity influenced enrollment and renewal of membership. Community-level factors such as culture and community involvement in scheme development influenced enrollment and sustainability of scheme. Systems-level factors encompassed governance, financial and delivery arrangement. Government involvement, accountability of scheme management, and strong policymaker-implementer relation facilitated implementation and sustainability of scheme. Packages that covered outpatient and inpatient care and those tailored to community needs contributed to increased enrollment. Amount and timing of premium collection was reported to negatively influence enrollment while factors reported as threats to sustainability included facility bankruptcy, operating on small budgets, rising healthcare costs, small risk pool, irregular contributions, and overutilization of services. At the delivery level, accessibility of facilities, facility environment, and health personnel influenced enrollment, service utilization and dropout rates. There are a multitude of interrelated factors at the individual, interpersonal, community and systems levels that drive the implementation, uptake and sustainability of CBHI schemes. We discuss the implications of the findings at the policy and research level. The review protocol is registered in PROSPERO International prospective register of systematic reviews (ID =  CRD42015019812 ).

The social benefits of WEEE re-use schemes. A cost benefit analysis for PCs in Spain.

PubMed

González, Xose Manuel; Rodríguez, Miguel; Pena-Boquete, Yolanda

2017-06-01

One goal of the new European legislation set out in WEEE Directive 2012/19/UE is the promotion of WEEE re-use schemes. However, some authors are rather sceptical about the contribution of WEEE re-use schemes to improve resource efficiency. In order to evaluate and to design adequate policy instruments, some authors recommend the cost-benefit analysis (CBA) as a compulsory first step. In this context, the main contribution of this paper is to enlarge the empirical literature by providing a CBA of re-use schemes versus recycling processes of PCs. The analysis is made for Spain by quantifying in monetary terms the social damages of environmental impacts such as climate change, human toxicity, particulate matter formation, metal depletion, etc. Our results suggest that promoting re-use against recycling (and consequently the need for manufacturing a new PC from raw materials) may reduce environmental costs by 45.20€ per PC. Those social benefits are mainly generated in the re-use preparation process and distribution activities, whereas the re-use scenario displays a worse performance in energy consumption. The difference in the distribution stage during the second life cycle originates from the fact that the ready to re-use product is produced locally, while the brand new product is manufactured and distributed from abroad, mainly Asia. These results provide valuable information to policymakers and think tanks willing to design support schemes for re-use over recycling operations. Copyright © 2017 Elsevier Ltd. All rights reserved.

A fast and efficient segmentation scheme for cell microscopic image.

PubMed

Lebrun, G; Charrier, C; Lezoray, O; Meurie, C; Cardot, H

2007-04-27

Microscopic cellular image segmentation schemes must be efficient for reliable analysis and fast to process huge quantity of images. Recent studies have focused on improving segmentation quality. Several segmentation schemes have good quality but processing time is too expensive to deal with a great number of images per day. For segmentation schemes based on pixel classification, the classifier design is crucial since it is the one which requires most of the processing time necessary to segment an image. The main contribution of this work is focused on how to reduce the complexity of decision functions produced by support vector machines (SVM) while preserving recognition rate. Vector quantization is used in order to reduce the inherent redundancy present in huge pixel databases (i.e. images with expert pixel segmentation). Hybrid color space design is also used in order to improve data set size reduction rate and recognition rate. A new decision function quality criterion is defined to select good trade-off between recognition rate and processing time of pixel decision function. The first results of this study show that fast and efficient pixel classification with SVM is possible. Moreover posterior class pixel probability estimation is easy to compute with Platt method. Then a new segmentation scheme using probabilistic pixel classification has been developed. This one has several free parameters and an automatic selection must dealt with, but criteria for evaluate segmentation quality are not well adapted for cell segmentation, especially when comparison with expert pixel segmentation must be achieved. Another important contribution in this paper is the definition of a new quality criterion for evaluation of cell segmentation. The results presented here show that the selection of free parameters of the segmentation scheme by optimisation of the new quality cell segmentation criterion produces efficient cell segmentation.

The social security scheme in Thailand: what lessons can be drawn?

PubMed

Tangcharoensathien, V; Supachutikul, A; Lertiendumrong, J

1999-04-01

The Social Security Scheme was launched in 1990, covering formal sector private employees for non-work related sickness, maternity and invalidity including cash benefits and funeral grants. The scheme is financed by tripartite contributions from government, employers and employees, each of 1.5% of payroll (total of 4.5%). The scheme decided to pay health care providers, whether public or private, on a flat rate capitation basis to cover both ambulatory and inpatient care. Registration of the insured with a contractor hospital was a necessary consequence of the chosen capitation payment system. The aim of this paper is to review the operation of the scheme, and to explore the implications of capitation payment and registration for utilisation levels and provider behaviour. A key weakness of the scheme's design is suggested to be the initial decision to give employers not employees the responsibility for choosing the registered hospitals. This was done for administrative reasons, but it contributed to low levels of use of the contractor hospitals. In addition, low levels of use were also probably the result of the potential for cream skimming, cost shifting from inpatient to ambulatory care and under-provision of patient care, though since monitoring mechanisms by the Social Security Office were weak, these effects are difficult to detect conclusively. Mechanisms to improve utilisation levels were gradually introduced, such as employee choice of registered hospitals and the formation of sub-contractor networks to improve access to care. A beneficial effect of the capitation payment system was that the Social Security Fund generated substantial reserves and expenditures on sickness benefits were well stabilised. The paper ends by recommending that future policy amendments should be guided by research and empirical findings and that tougher monitoring and enforcement of quality of care standards are required.

[Extension of health coverage and community based health insurance schemes in Africa: Myths and realities].

PubMed

Boidin, B

2015-02-01

This article tackles the perspectives and limits of the extension of health coverage based on community based health insurance schemes in Africa. Despite their strong potential contribution to the extension of health coverage, their weaknesses challenge their ability to play an important role in this extension. Three limits are distinguished: financial fragility; insufficient adaptation to characteristics and needs of poor people; organizational and institutional failures. Therefore lessons can be learnt from the limits of the institutionalization of community based health insurance schemes. At first, community based health insurance schemes are to be considered as a transitional but insufficient solution. There is also a stronger role to be played by public actors in improving financial support, strengthening health services and coordinating coverage programs.

Dynamic Non-Hierarchical File Systems for Exascale Storage

DOE Office of Scientific and Technical Information (OSTI.GOV)

Long, Darrell E.; Miller, Ethan L

This constitutes the final report for “Dynamic Non-Hierarchical File Systems for Exascale Storage”. The ultimate goal of this project was to improve data management in scientific computing and high-end computing (HEC) applications, and to achieve this goal we proposed: to develop the first, HEC-targeted, file system featuring rich metadata and provenance collection, extreme scalability, and future storage hardware integration as core design goals, and to evaluate and develop a flexible non-hierarchical file system interface suitable for providing more powerful and intuitive data management interfaces to HEC and scientific computing users. Data management is swiftly becoming a serious problem in themore » scientific community – while copious amounts of data are good for obtaining results, finding the right data is often daunting and sometimes impossible. Scientists participating in a Department of Energy workshop noted that most of their time was spent “...finding, processing, organizing, and moving data and it’s going to get much worse”. Scientists should not be forced to become data mining experts in order to retrieve the data they want, nor should they be expected to remember the naming convention they used several years ago for a set of experiments they now wish to revisit. Ideally, locating the data you need would be as easy as browsing the web. Unfortunately, existing data management approaches are usually based on hierarchical naming, a 40 year-old technology designed to manage thousands of files, not exabytes of data. Today’s systems do not take advantage of the rich array of metadata that current high-end computing (HEC) file systems can gather, including content-based metadata and provenance1 information. As a result, current metadata search approaches are typically ad hoc and often work by providing a parallel management system to the “main” file system, as is done in Linux (the locate utility), personal computers, and enterprise search appliances. These search applications are often optimized for a single file system, making it difficult to move files and their metadata between file systems. Users have tried to solve this problem in several ways, including the use of separate databases to index file properties, the encoding of file properties into file names, and separately gathering and managing provenance data, but none of these approaches has worked well, either due to limited usefulness or scalability, or both. Our research addressed several key issues: High-performance, real-time metadata harvesting: extracting important attributes from files dynamically and immediately updating indexes used to improve search; Transparent, automatic, and secure provenance capture: recording the data inputs and processing steps used in the production of each file in the system; Scalable indexing: indexes that are optimized for integration with the file system; Dynamic file system structure: our approach provides dynamic directories similar to those in semantic file systems, but these are the native organization rather than a feature grafted onto a conventional system. In addition to these goals, our research effort will include evaluating the impact of new storage technologies on the file system design and performance. In particular, the indexing and metadata harvesting functions can potentially benefit from the performance improvements promised by new storage class memories.« less

A Description of Exotic Nuclei by the Aid of Virtual States

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vertse, T.; University of Debrecen, Faculty of Information Science, H-4010 Debrecen, Pf. 12; Id Betan, R.

2005-11-21

A new unified shell model scheme is introduced in which the single particle basis is a generalization of the Berggren representation. This repesentation includes virtual i.e. antibound basis states as well. We apply the new scheme to the ground state of the exotic nucleus 11Li. Here the antibound pole and the complex continuum both have large contributions and neither of them can be neglected.

An atomic mean-field spin-orbit approach within exact two-component theory for a non-perturbative treatment of spin-orbit coupling

NASA Astrophysics Data System (ADS)

Liu, Junzi; Cheng, Lan

2018-04-01

An atomic mean-field (AMF) spin-orbit (SO) approach within exact two-component theory (X2C) is reported, thereby exploiting the exact decoupling scheme of X2C, the one-electron approximation for the scalar-relativistic contributions, the mean-field approximation for the treatment of the two-electron SO contribution, and the local nature of the SO interactions. The Hamiltonian of the proposed SOX2CAMF scheme comprises the one-electron X2C Hamiltonian, the instantaneous two-electron Coulomb interaction, and an AMF SO term derived from spherically averaged Dirac-Coulomb Hartree-Fock calculations of atoms; no molecular relativistic two-electron integrals are required. Benchmark calculations for bond lengths, harmonic frequencies, dipole moments, and electric-field gradients for a set of diatomic molecules containing elements across the periodic table show that the SOX2CAMF scheme offers a balanced treatment for SO and scalar-relativistic effects and appears to be a promising candidate for applications to heavy-element containing systems. SOX2CAMF coupled-cluster calculations of molecular properties for bismuth compounds (BiN, BiP, BiF, BiCl, and BiI) are also presented and compared with experimental results to further demonstrate the accuracy and applicability of the SOX2CAMF scheme.

Prediction-based association control scheme in dense femtocell networks.

PubMed

Sung, Nak Woon; Pham, Ngoc-Thai; Huynh, Thong; Hwang, Won-Joo; You, Ilsun; Choo, Kim-Kwang Raymond

2017-01-01

The deployment of large number of femtocell base stations allows us to extend the coverage and efficiently utilize resources in a low cost manner. However, the small cell size of femtocell networks can result in frequent handovers to the mobile user, and consequently throughput degradation. Thus, in this paper, we propose predictive association control schemes to improve the system's effective throughput. Our design focuses on reducing handover frequency without impacting on throughput. The proposed schemes determine handover decisions that contribute most to the network throughput and are proper for distributed implementations. The simulation results show significant gains compared with existing methods in terms of handover frequency and network throughput perspective.

Absence of even-integer ζ-function values in Euclidean physical quantities in QCD

NASA Astrophysics Data System (ADS)

Jamin, Matthias; Miravitllas, Ramon

2018-04-01

At order αs4 in perturbative quantum chromodynamics, even-integer ζ-function values are present in Euclidean physical correlation functions like the scalar quark correlation function or the scalar gluonium correlator. We demonstrate that these contributions cancel when the perturbative expansion is expressed in terms of the so-called C-scheme coupling αˆs which has recently been introduced in Ref. [1]. It is furthermore conjectured that a ζ4 term should arise in the Adler function at order αs5 in the MS ‾-scheme, and that this term is expected to disappear in the C-scheme as well.

Transmutation of fullerenes.

PubMed

Cross, R James; Saunders, Martin

2005-03-09

Fullerenes were pyrolyzed by subliming them into a stream of flowing argon gas and then passing them through an oven heated to approximately 1000 degrees C. C(76), C(78), and C(84) all readily lost carbons to form smaller fullerenes. In the case of C(78), some isomerization was seen. Pyrolysis of (3)He@C(76) showed that all or most of the (3)He was lost during the decomposition. C(60) passes through the apparatus with no decomposition and no loss of helium.

Combining Hydrological Modeling and Remote Sensing Observations to Enable Data-Driven Decision Making for Devils Lake Flood Mitigation in a Changing Climate

NASA Technical Reports Server (NTRS)

Zhang, Xiaodong; Kirilenko, Andrei; Lim, Howe; Teng, Williams

2010-01-01

This slide presentation reviews work to combine the hydrological models and remote sensing observations to monitor Devils Lake in North Dakota, to assist in flood damage mitigation. This reports on the use of a distributed rainfall-runoff model, HEC-HMS, to simulate the hydro-dynamics of the lake watershed, and used NASA's remote sensing data, including the TRMM Multi-Satellite Precipitation Analysis (TMPA) and AIRS surface air temperature, to drive the model.

Chicago’s O’Hare Runway Configuration Management System (RCMS). Volume 1. Description of the Operational Software,

DTIC Science & Technology

1987-07-01

9 ...Glossary D-i E- Distribution List E-I D. tSl ’S I hec 3 tttt ,"t!<_-Z. :,,’ e ’: :’ :.,", : - ’,,"/ . .>" ",;’ 9 ’ ’:/’,"" ’,,-’¢’..’:, : ,’-.’ ,.-;.;.;- 4...2 Sheets) 23 6 Foreground Program Flowchart (2 Sheets) 25 7 PF-1 Master Auto Panel 27 8 PF-2 Master Panel 28 9 PF-3 Eligible Current

An Objective Summary of US Army Electro-Optical Modeling and Field Testing in an Obscuring Environment.

DTIC Science & Technology

1981-10-01

NUMBER 7. AUTHOR( e ) 8. CONTRACT OR GRANT NUMBER(&) Robert A. Sutherland Donald W. Hoock Rirhard R r.nM NA 9. PERFORMING ORGANIZATION NAME AND ADDRESS...smoke produced by a burning tank (BURN), another treats fires in general (FIRE), four are inventory smoke munitions expenditure models ( STILES , SEMM... E . Cramer (HEC) Company under contract to ASL and Tforms the basic transport and diffusion routine for the larger system called Experimental Prototype

Fair ranking of researchers and research teams.

PubMed

Vavryčuk, Václav

2018-01-01

The main drawback of ranking of researchers by the number of papers, citations or by the Hirsch index is ignoring the problem of distributing authorship among authors in multi-author publications. So far, the single-author or multi-author publications contribute to the publication record of a researcher equally. This full counting scheme is apparently unfair and causes unjust disproportions, in particular, if ranked researchers have distinctly different collaboration profiles. These disproportions are removed by less common fractional or authorship-weighted counting schemes, which can distribute the authorship credit more properly and suppress a tendency to unjustified inflation of co-authors. The urgent need of widely adopting a fair ranking scheme in practise is exemplified by analysing citation profiles of several highly-cited astronomers and astrophysicists. While the full counting scheme often leads to completely incorrect and misleading ranking, the fractional or authorship-weighted schemes are more accurate and applicable to ranking of researchers as well as research teams. In addition, they suppress differences in ranking among scientific disciplines. These more appropriate schemes should urgently be adopted by scientific publication databases as the Web of Science (Thomson Reuters) or the Scopus (Elsevier).

Contribution of bioaerosols to the global organic aerosol budget

NASA Astrophysics Data System (ADS)

Janssen, R.; Heald, C. L.

2017-12-01

Bioaerosols are ubiquitous in the atmosphere and may contribute significantly to cloud condensation and ice nuclei populations, and consequently to formation of clouds and precipitation. However, quantifying the contribution of bioaerosols to the global organic aerosol budget is a challenge, since bioaerosol sources are poorly constrained on the global scale. Previous global estimates of global primary biological aerosol particle (PBAP) emissions, including bacteria, fungal spores and pollen, range from 78-296 Tg/year. Over the past several years, size-resolved measurements of fluorescent biological aerosol particles have been made in tropical, temperate and boreal ecosystems. Besides, single particle mass spectroscopy has been used to quantify bioaerosol concentrations at various locations in the US. We use these observations to develop and evaluate a bioaerosol emission scheme that describes both the biological production of bioaerosols and the meteorological drivers of the emission of these particles into the atmosphere. Then, we implement this scheme in the GEOS-Chem global chemical transport model to estimate the emission, burden and lifetime of bioaerosols. Finally, we evaluate the contribution of bioaerosols to the total organic aerosol budget, which further consists of primary and secondary organic aerosol.

DREAM: Classification scheme for dialog acts in clinical research query mediation.

PubMed

Hoxha, Julia; Chandar, Praveen; He, Zhe; Cimino, James; Hanauer, David; Weng, Chunhua

2016-02-01

Clinical data access involves complex but opaque communication between medical researchers and query analysts. Understanding such communication is indispensable for designing intelligent human-machine dialog systems that automate query formulation. This study investigates email communication and proposes a novel scheme for classifying dialog acts in clinical research query mediation. We analyzed 315 email messages exchanged in the communication for 20 data requests obtained from three institutions. The messages were segmented into 1333 utterance units. Through a rigorous process, we developed a classification scheme and applied it for dialog act annotation of the extracted utterances. Evaluation results with high inter-annotator agreement demonstrate the reliability of this scheme. This dataset is used to contribute preliminary understanding of dialog acts distribution and conversation flow in this dialog space. Copyright © 2015 Elsevier Inc. All rights reserved.

Multi-criteria decision-making on assessment of proposed tidal barrage schemes in terms of environmental impacts.

PubMed

Wu, Yunna; Xu, Chuanbo; Ke, Yiming; Chen, Kaifeng; Xu, Hu

2017-12-15

For tidal range power plants to be sustainable, the environmental impacts caused by the implement of various tidal barrage schemes must be assessed before construction. However, several problems exist in the current researches: firstly, evaluation criteria of the tidal barrage schemes environmental impact assessment (EIA) are not adequate; secondly, uncertainty of criteria information fails to be processed properly; thirdly, correlation among criteria is unreasonably measured. Hence the contributions of this paper are as follows: firstly, an evaluation criteria system is established from three dimensions of hydrodynamic, biological and morphological aspects. Secondly, cloud model is applied to describe the uncertainty of criteria information. Thirdly, Choquet integral with respect to λ-fuzzy measure is introduced to measure the correlation among criteria. On the above bases, a multi-criteria decision-making decision framework for tidal barrage scheme EIA is established to select the optimal scheme. Finally, a case study demonstrates the effectiveness of the proposed framework. Copyright © 2017 Elsevier Ltd. All rights reserved.

Technical note: Improving the AWAT filter with interpolation schemes for advanced processing of high resolution data

NASA Astrophysics Data System (ADS)

Peters, Andre; Nehls, Thomas; Wessolek, Gerd

2016-06-01

Weighing lysimeters with appropriate data filtering yield the most precise and unbiased information for precipitation (P) and evapotranspiration (ET). A recently introduced filter scheme for such data is the AWAT (Adaptive Window and Adaptive Threshold) filter (Peters et al., 2014). The filter applies an adaptive threshold to separate significant from insignificant mass changes, guaranteeing that P and ET are not overestimated, and uses a step interpolation between the significant mass changes. In this contribution we show that the step interpolation scheme, which reflects the resolution of the measuring system, can lead to unrealistic prediction of P and ET, especially if they are required in high temporal resolution. We introduce linear and spline interpolation schemes to overcome these problems. To guarantee that medium to strong precipitation events abruptly following low or zero fluxes are not smoothed in an unfavourable way, a simple heuristic selection criterion is used, which attributes such precipitations to the step interpolation. The three interpolation schemes (step, linear and spline) are tested and compared using a data set from a grass-reference lysimeter with 1 min resolution, ranging from 1 January to 5 August 2014. The selected output resolutions for P and ET prediction are 1 day, 1 h and 10 min. As expected, the step scheme yielded reasonable flux rates only for a resolution of 1 day, whereas the other two schemes are well able to yield reasonable results for any resolution. The spline scheme returned slightly better results than the linear scheme concerning the differences between filtered values and raw data. Moreover, this scheme allows continuous differentiability of filtered data so that any output resolution for the fluxes is sound. Since computational burden is not problematic for any of the interpolation schemes, we suggest always using the spline scheme.

Two particle tracking and detection in a single Gaussian beam optical trap.

PubMed

Praveen, P; Yogesha; Iyengar, Shruthi S; Bhattacharya, Sarbari; Ananthamurthy, Sharath

2016-01-20

We have studied in detail the situation wherein two microbeads are trapped axially in a single-beam Gaussian intensity profile optical trap. We find that the corner frequency extracted from a power spectral density analysis of intensity fluctuations recorded on a quadrant photodetector (QPD) is dependent on the detection scheme. Using forward- and backscattering detection schemes with single and two laser wavelengths along with computer simulations, we conclude that fluctuations detected in backscattering bear true position information of the bead encountered first in the beam propagation direction. Forward scattering, on the other hand, carries position information of both beads with substantial contribution from the bead encountered first along the beam propagation direction. Mie scattering analysis further reveals that the interference term from the scattering of the two beads contributes significantly to the signal, precluding the ability to resolve the positions of the individual beads in forward scattering. In QPD-based detection schemes, detection through backscattering, thereby, is imperative to track the true displacements of axially trapped microbeads for possible studies on light-mediated interbead interactions.

Flood Study of Warren Brook in Alstead and Cold River in Alstead, Langdon, and Walpole, New Hampshire, 2005

USGS Publications Warehouse

Flynn, Robert H.

2006-01-01

This report presents water-surface elevations and profiles as determined using the U.S. Army Corps of Engineers (USACE) one-dimensional Hydrologic Engineering Center River Analysis System, also known as HEC-RAS. Steady flow water-surface profiles were developed for two stream reaches: the Cold River from its confluence with the Connecticut River in Walpole, through Alstead to the McDermott Bridge in Langdon, NH, and Warren Brook from its confluence with the Cold River to Warren Lake in Alstead, NH. Flood events of a magnitude, which are expected to be equaled or exceeded once on the average during any 10-, 50-, 100-, or 500-year period (recurrence interval), were modeled using HEC-RAS as these flood events are recognized as being significant for flood-plain management, determination of flood insurance rates, and design of structures such as bridges and culverts. These flood events are referred to as the 10-, 50-, 100-, and 500-year floods and have a 10-, 2-, 1-, and 0.2-percent chance, respectively, of being equaled or exceeded during any year. The recurrence intervals represent the long-term average between floods of a specific magnitude. The risk of experiencing rare floods at short intervals or within the same year increases when periods greater than one year are considered. The analyses in this study reflect the flooding potentials based on conditions existing in the communities of Walpole, Alstead and Langdon at the time of completion of this study.

Application of HEC-RAS for flood forecasting in perched river-A case study of hilly region, China

NASA Astrophysics Data System (ADS)

Sun, Pingping; Wang, Shuqian; Gan, Hong; Liu, Bin; Jia, Ling

2017-04-01

Flooding in small and medium rivers are seriously threatening the safety of human beings’ life and property. The simulation forecasting of the river flood and bank risk in hilly region has gradually become a hotspot. At present, there are few studies on the simulation of hilly perched river, especially in the case of lacking section flow data. And the method of how to determine the position of the levee breach along the river bank is not much enough. Based on the characteristics of the sections in hilly perched river, an attempt is applied in this paper which establishes the correlation between the flow profile computed by HEC-RAS model and the river bank. A hilly perched river in Lingshi County, Shanxi Province of China, is taken as the study object, the levee breach positions along the bank are simulated under four different design storm. The results show that the flood control standard of upper reach is high, which can withstand the design storm of 100 years. The current standard of lower reach is low, which is the flooding channel with high frequency. As the standard of current channel between the 2rd and the 11th section is low, levee along that channel of the river bank is considered to be heighten and reinforced. The study results can provide some technical support for flood proofing in hilly region and some reference for the reinforcement of river bank.

Assessment Approach for Identifying Compatibility of Restoration Projects with Geomorphic and Flooding Processes in Gravel Bed Rivers

NASA Astrophysics Data System (ADS)

DeVries, Paul; Aldrich, Robert

2015-08-01

A critical requirement for a successful river restoration project in a dynamic gravel bed river is that it be compatible with natural hydraulic and sediment transport processes operating at the reach scale. The potential for failure is greater at locations where the influence of natural processes is inconsistent with intended project function and performance. We present an approach using practical GIS, hydrologic, hydraulic, and sediment transport analyses to identify locations where specific restoration project types have the greatest likelihood of working as intended because their function and design are matched with flooding and morphologic processes. The key premise is to identify whether a specific river analysis segment (length ~1-10 bankfull widths) within a longer reach is geomorphically active or inactive in the context of vertical and lateral stabilities, and hydrologically active for floodplain connectivity. Analyses involve empirical channel geometry relations, aerial photographic time series, LiDAR data, HEC-RAS hydraulic modeling, and a time-integrated sediment transport budget to evaluate trapping efficiency within each segment. The analysis segments are defined by HEC-RAS model cross sections. The results have been used effectively to identify feasible projects in a variety of alluvial gravel bed river reaches with lengths between 11 and 80 km and 2-year flood magnitudes between ~350 and 1330 m3/s. Projects constructed based on the results have all performed as planned. In addition, the results provide key criteria for formulating erosion and flood management plans.

Assessment Approach for Identifying Compatibility of Restoration Projects with Geomorphic and Flooding Processes in Gravel Bed Rivers.

PubMed

DeVries, Paul; Aldrich, Robert

2015-08-01

A critical requirement for a successful river restoration project in a dynamic gravel bed river is that it be compatible with natural hydraulic and sediment transport processes operating at the reach scale. The potential for failure is greater at locations where the influence of natural processes is inconsistent with intended project function and performance. We present an approach using practical GIS, hydrologic, hydraulic, and sediment transport analyses to identify locations where specific restoration project types have the greatest likelihood of working as intended because their function and design are matched with flooding and morphologic processes. The key premise is to identify whether a specific river analysis segment (length ~1-10 bankfull widths) within a longer reach is geomorphically active or inactive in the context of vertical and lateral stabilities, and hydrologically active for floodplain connectivity. Analyses involve empirical channel geometry relations, aerial photographic time series, LiDAR data, HEC-RAS hydraulic modeling, and a time-integrated sediment transport budget to evaluate trapping efficiency within each segment. The analysis segments are defined by HEC-RAS model cross sections. The results have been used effectively to identify feasible projects in a variety of alluvial gravel bed river reaches with lengths between 11 and 80 km and 2-year flood magnitudes between ~350 and 1330 m(3)/s. Projects constructed based on the results have all performed as planned. In addition, the results provide key criteria for formulating erosion and flood management plans.

D¯0D0* (D0D¯0*) system in QCD-improved many body potential

NASA Astrophysics Data System (ADS)

Jamil, M. Imran; Masud, Bilal; Akram, Faisal; Sohail Gilani, S. M.

2017-01-01

For a system of current interest (composed of charm, anticharm and a pair of light quarks), we show trends in phenomenological implications of QCD-based improvements to a simple quark model treatment. We employ a resonating group method to render this difficult four-body problem manageable. We use a quadratic confinement so as to be able to improve beyond the Born approximation. We report the position of the pole corresponding to the D¯0D0* molecule for the best fit of a model parameter to the relevant QCD simulations. We point out the interesting possibility that the pole can be shifted to 3872 MeV by introducing another parameter I 0 that changes the strength of the interaction in this one component of X(3872). The revised value of this second parameter can guide future trends in modeling of the full exotic meson X(3872). We also report the changes with I 0 in the S-wave spin averaged cross sections for D¯0D0* ⟶ ωJ/ψ and D¯0D0* ⟶ ρJ/ψ. These cross sections are important regarding the study of QGP (quark gluon plasma). BM and FA acknowledge the support of PU research (D/605/Est.I Sr. 20 Project 2014-15, D/34/Est.1 Sr. 109 Project 2013-14), SG is thankful to the Higher Education Commission (HEC) of Pakistan for its financial support through (17-5-4(Ps3-128) HEC/Sch/2006)

Thiolated hydroxyethyl cellulose: design and in vitro evaluation of mucoadhesive and permeation enhancing nanoparticles.

PubMed

Rahmat, Deni; Müller, Christiane; Barthelmes, Jan; Shahnaz, Gul; Martien, Ronny; Bernkop-Schnürch, Andreas

2013-02-01

Within this study, HEC-cysteamine nanoparticles with free thiol groups in the range of 117-1548 μmol/g were designed and characterized. Nanoparticles were generated via ionic gelation of the cationic polymer with tripolyphosphate (TPP) followed by covalent crosslinking via disulfide bond formation using H2O2 as oxidant. The mean diameter of the particles was in the range of 270-360 nm, and zeta potential was determined to be +4 to +10 mV. Nanoparticles were evaluated in terms of mucoadhesive, permeation enhancing, and biocompatible properties as well as biodegradability. The particles remained attached to porcine intestinal mucosa up to 70% after 3h of incubation. The more nanoparticles were oxidized; however, the less were their mucoadhesive properties. Nanoparticles applied in a concentration of 0.5% (m/v) with the highest content of free thiol groups improved the transport of fluorescein isothiocyanate dextran 4 (FD4) across Caco-2 cell monolayer 3.94-fold in comparison with control (buffer). In addition, the transport of FD4 was even 1.84-fold enhanced in the presence of 0.5% (m/v) nanoparticles with the lowest free thiol group content. The higher the disulfide bond content within nanoparticles was, to a lower degree nanoparticles were hydrolyzed by cellulase. None of these nanoparticles showed pronounced cytotoxicity. Accordingly, HEC-cysteamine could be a promising excipient for nanoparticulate delivery systems for poorly absorbed drugs. Copyright © 2012 Elsevier B.V. All rights reserved.

Retinoic Acid Receptor β: A Potential Therapeutic Target in Retinoic Acid Treatment of Endometrial Cancer.

PubMed

Tsuji, Keita; Utsunomiya, Hiroki; Miki, Yasuhiro; Hanihara, Mayu; Fue, Misaki; Takagi, Kiyoshi; Nishimoto, Mitsuo; Suzuki, Fumihiko; Yaegashi, Nobuo; Suzuki, Takashi; Ito, Kiyoshi

2017-05-01

Several studies have reported that retinoic acid (RA) might be used to treat malignancies. The effects of RA are mediated by the RA receptor (RAR), and RARα/RARβ especially acts as a tumor suppressor. However, little is known about its role in human endometrial cancer. In this study, we examined the effects of all-trans RA (ATRA) on progression of human endometrial cancer cell line, RL95-2 and Hec1A. We then examined the expression of RARα and RARβ in 50 endometrial cancer tissues by using immunohistochemistry. We found inhibitory effects of ATRA on cell proliferation, apoptosis, and migration in RL95-2 cells, but not in Hec1A cells. RARα or RARβ knockdown individually could not cancel out the inhibition of cell proliferation by ATRA in RL95-2 cells, but simultaneous knockdown of RARα and RARβ could block its effect on proliferation. RARα and RARβ knockdown dose dependently reduced the inhibition of migration by ATRA, but the effect was more pronounced with RARβ knockdown than with RARα knockdown. We confirmed that RARβ gene was directly regulated by ATRA in microarray and real-time reverse transcription polymerase chain reaction. Furthermore, the RARβ agonist (BMS453) significantly suppressed proliferation of RL95-2 cells. In immunohistochemical analysis, RARα expression was positively correlated with tumor grade, and RARβ showed the opposite tendency in endometrial cancer. Retinoic acid might have multiple antitumor effects, and RARβ may be a potent therapeutic target in RA treatment for endometrial cancers.

A coupled hydrological-hydraulic flood inundation model calibrated using post-event measurements and integrated uncertainty analysis in a poorly gauged Mediterranean basin

NASA Astrophysics Data System (ADS)

Hdeib, Rouya; Abdallah, Chadi; Moussa, Roger; Colin, Francois

2017-04-01

Developing flood inundation maps of defined exceedance probabilities is required to provide information on the flood hazard and the associated risk. A methodology has been developed to model flood inundation in poorly gauged basins, where reliable information on the hydrological characteristics of floods are uncertain and partially captured by the traditional rain-gauge networks. Flood inundation is performed through coupling a hydrological rainfall-runoff (RR) model (HEC-HMS) with a hydraulic model (HEC-RAS). The RR model is calibrated against the January 2013 flood event in the Awali River basin, Lebanon (300 km2), whose flood peak discharge was estimated by post-event measurements. The resulting flows of the RR model are defined as boundary conditions of the hydraulic model, which is run to generate the corresponding water surface profiles and calibrated against 20 post-event surveyed cross sections after the January-2013 flood event. An uncertainty analysis is performed to assess the results of the models. Consequently, the coupled flood inundation model is simulated with design storms and flood inundation maps are generated of defined exceedance probabilities. The peak discharges estimated by the simulated RR model were in close agreement with the results from different empirical and statistical methods. This methodology can be extended to other poorly gauged basins facing common stage-gauge failure or characterized by floods with a stage exceeding the gauge measurement level, or higher than that defined by the rating curve.

Curcumin exhibits anti-tumor effect and attenuates cellular migration via Slit-2 mediated down-regulation of SDF-1 and CXCR4 in endometrial adenocarcinoma cells.

PubMed

Sirohi, Vijay Kumar; Popli, Pooja; Sankhwar, Pushplata; Kaushal, Jyoti Bala; Gupta, Kanchan; Manohar, Murli; Dwivedi, Anila

2017-06-01

Although curcumin shows anti-proliferative and anti-inflammatory activities in various cancers, the effect of curcumin on cellular migration in endometrial adenocarcinoma cells remains to be understood. The current investigation was aimed to explore the anti-proliferative and anti-migratory effects of curcumin and its mechanism of action in endometrial cancer cells. Our in-vitro and in-vivo experimental studies showed that curcumin inhibited the proliferation of endometrial cancer cells and suppressed the tumor growth in Ishikawa xenograft mouse model. Curcumin induced ROS-mediated apoptosis in endometrial cancer cells. Curcumin suppressed the migration rate of Ishikawa and Hec-1B cells as analyzed by scratch wound assay. In transwell migration studies, knock down of Slit-2 reversed the anti-migratory effect of curcumin in these cell lines. Curcumin significantly up-regulated the expression of Slit-2 in Ishikawa, Hec-1B and primary endometrial cancer cells while it down-regulated the expression of stromal cell-derived factor-1 (SDF-1) and CXCR4 which in turn, suppressed the expression of matrix metallopeptidases (MMP) 2 and 9, thus attenuating the migration of endometrial cancer cells. In summary, we have demonstrated that curcumin has inhibitory effect on cellular migration via Slit-2 mediated down-regulation of CXCR4, SDF-1, and MMP2/MMP9 in endometrial carcinoma cells. These findings helped explore the role of Slit-2 in endometrial cancer cells. Copyright © 2017 Elsevier Inc. All rights reserved.

Exploring the Behavior and Metabolic Transformations of SeNPs in Exposed Lactic Acid Bacteria. Effect of Nanoparticles Coating Agent

PubMed Central

Palomo-Siguero, Maria; Madrid, Yolanda

2017-01-01

The behavior and transformation of selenium nanoparticles (SeNPs) in living systems such as microorganisms is largely unknown. To address this knowledge gap, we examined the effect of three types of SeNP suspensions toward Lactobacillus delbrueckii subsp. bulgaricus LB-12 using a variety of techniques. SeNPs were synthesized using three types of coating agents (chitosan (CS-SeNPs), hydroxyethyl cellulose (HEC-SeNPs) and a non-ionic surfactant, surfynol (ethoxylated-SeNPs)). Morphologies of SeNPs were all spherical. Transmission electron microscopy (TEM) was used to locate SeNPs in the bacteria. High performance liquid chromatography (HPLC) on line coupled to inductively coupled plasma mass spectrometry (ICP-MS) was applied to evaluate SeNP transformation by bacteria. Finally, flow cytometry employing the live/dead test and optical density measurements at 600 nm (OD600) were used for evaluating the percentages of bacteria viability when supplementing with SeNPs. Negligible damage was detected by flow cytometry when bacteria were exposed to HEC-SeNPs or CS-SeNPs at a level of 10 μg Se mL−1. In contrast, ethoxylated-SeNPs were found to be the most harmful nanoparticles toward bacteria. CS-SeNPs passed through the membrane without causing damage. Once inside, SeNPs were metabolically transformed to organic selenium compounds. Results evidenced the importance of capping agents when establishing the true behavior of NPs. PMID:28783048

Displacement rates on the Toroweap and Hurricane faults: implications for Quaternary downcutting in the Grand Canyon, Arizona

USGS Publications Warehouse

Fenton, Cassandra R.; Webb, Robert H.; Pearthree, Philip A.; Cerling, Thure E.; Poreda, Robert J.

2001-01-01

The Toroweap and Hurricane faults, considered to be the most active in Arizona, cross the Uinkaret volcanic field in the western Grand Canyon. These normal faults are downthrown to the west, and the Colorado River crosses these faults as it flows west in the Grand Canyon. Cosmogenic 3He (3Hec) dates on basalt flows and related landforms are used to calculate vertical displacement rates for these faults. The two faults cross unruptured alluvial fans dated as 3 ka (Toroweap) and 8 ka (Hurricane), and 10 other landforms that range in age from 30 to 400 ka are displaced. Middle and late Quaternary displacement rates of the Toroweap and Hurricane faults are 70–180 and 70–170 m/m.y., respectively. On the basis of these rates, the combined displacement of 580 m on these faults could have occurred in the past 3 to 5 m.y. All 3Hec dates are younger than existing K- Ar dates and are consistent with new 40Ar/39Ar dates and existing thermoluminescence (TL) dates on basalt flows. These different dating techniques may be combined in an analysis of displacement rates. Downcutting rates for the Colorado River in the eastern Grand Canyon (400 m/m.y.) are at least double the downcutting rates west of the faults (70–160 m/m.y.). Faulting probably increased downcutting in the eastern Grand Canyon relative to downcutting in the western Grand Canyon during the late Quaternary.

Prevalence and trends of cellulosics in pharmaceutical dosage forms.

PubMed

Mastropietro, David J; Omidian, Hossein

2013-02-01

Many studies have shown that cellulose derivatives (cellulosics) can provide various benefits when used in virtually all types of dosage forms. Nevertheless, the popularity of their use in approved drug products is rather unknown. This research reports the current prevalence and trends of use for 15 common cellulosics in prescription drug products. The cellulosics were powdered and microcrystalline cellulose (MCC), ethyl cellulose, hydroxypropyl cellulose (HPC), hydroxyethyl cellulose (HEC), hypromellose (HPMC), HPMC phthalate, HPMC acetate succinate, cellulose acetate (CA), CA phthalate, sodium (Na) and calcium (Ca) carboxymethylcellulose (CMC), croscarmellose sodium (XCMCNa), methyl cellulose, and low substituted HPC. The number of brand drug products utilizing each cellulosics was determined using the online drug index Rxlist. A total of 607 brand products were identified having one or more of the cellulosics as an active or inactive ingredient. An array of various dosage forms was identified and revealed HPMC and MCC to be the most utilized cellulosics in all products followed by XCMCNa and HPC. Many products contained two or more cellulosics in the formulation (42% containing two, 23% containing three, and 4% containing 4-5). The largest combination occurrence was HPMC with MCC. The use of certain cellulosics within different dosage form types was found to contain specific trends. All injectables utilized only CMCNa, and the same with all ophthalmic solutions utilizing HPMC, and otic suspensions utilizing HEC. Popularity and trends regarding cellulosics use may occur based on many factors including functionality, safety, availability, stability, and ease of manufacturing.

Flood Discharge Analysis with Nakayasu Method Using Combination of HEC-RAS Method on Deli River in Medan City

NASA Astrophysics Data System (ADS)

Harahap, Rumilla; Jeumpa, Kemala; Hadibroto, Bambang

2018-03-01

The problem in this research is how in the rainy season the water does not overflow, does not occur flood and during the dry season does not occur drought so it can adjust the condition or existence of Deli river which is around Medan city. Deli River floods often occur, either caused by a smaller capacity than the existing discharge, lack of maintenance and drainage and disposal systems that do not fit with the environment, resulting in flood subscriptions every year. The purpose of this research is to know flood discharge at Deli river as Flood control in Medan city. This research is analyzed on several methods such as log Pearson, Gumbel and hydrograph unit, while HEC-RAS method is modeling conducted in analyzing the water profile of the Deli River. Furthermore, the calculation of the periodic flood discharge using the Nakayasu Method. Calculation result at Deli River return period flood discharge 2 years with an area of 14.8 km2 annual flood hydrograph the total is 26.79 m3/sec on the hours at the 4th time. Return period flood discharge 5 years with an area of 14.8 km2 annual flood hydrograph the total is 73,44 m3/sec. While 25 annual return period total flood hydrograph is 146.50 m3/sec. With flood analysis can reduce and minimize the risk of losses and land can be mapped if in the area there is flooding.

Small Molecule TH-39 Potentially Targets Hec1/Nek2 Interaction and Exhibits Antitumor Efficacy in K562 Cells via G0/G1 Cell Cycle Arrest and Apoptosis Induction.

PubMed

Zhu, Yongxia; Wei, Wei; Ye, Tinghong; Liu, Zhihao; Liu, Li; Luo, Yong; Zhang, Lidan; Gao, Chao; Wang, Ningyu; Yu, Luoting

2016-01-01

Cancer is still a major public health issue worldwide, and new therapeutics with anti-tumor activity are still urgently needed. The anti-tumor activity of TH-39, which shows potent anti-proliferative activity against K562 cells with an IC50 of 0.78 µM, was investigated using immunoblot, co-immunoprecipitation, the MTT assay, and flow cytometry. Mechanistically, TH-39 may disrupt the interaction between Hec1 and Nek2 in K562 cells. Moreover, TH-39 inhibited cell proliferation in a concentration- and time-dependent manner by influencing the morphology of K562 cells and inducing G0/G1 phase arrest. G0/G1 phase arrest was associated with down-regulation of CDK2-cyclin E complex and CDK4/6-cyclin D complex activities. Furthermore, TH-39 also induced cell apoptosis, which was associated with activation of caspase-3, down-regulation of Bcl-2 expression and up-regulation of Bax. TH-39 could also decrease mitochondrial membrane potential (Δψm) and increase reactive oxygen species (ROS) accumulation in K562 cells. The results indicated that TH-39 might induce apoptosis via the ROS-mitochondrial apoptotic pathway. This study highlights the potential therapeutic efficacy of the anti-cancer compound TH-39 in treatment-resistant chronic myeloid leukemia. © 2016 The Author(s) Published by S. Karger AG, Basel.

Comparing the Hydrologic and Watershed Processes between a Full Scale Stochastic Model Versus a Scaled Physical Model of Bell Canyon

NASA Astrophysics Data System (ADS)

Hernandez, K. F.; Shah-Fairbank, S.

2016-12-01

The San Dimas Experimental Forest has been designated as a research area by the United States Forest Service for use as a hydrologic testing facility since 1933 to investigate watershed hydrology of the 27 square mile land. Incorporation of a computer model provides validity to the testing of the physical model. This study focuses on San Dimas Experimental Forest's Bell Canyon, one of the triad of watersheds contained within the Big Dalton watershed of the San Dimas Experimental Forest. A scaled physical model was constructed of Bell Canyon to highlight watershed characteristics and each's effect on runoff. The physical model offers a comprehensive visualization of a natural watershed and can vary the characteristics of rainfall intensity, slope, and roughness through interchangeable parts and adjustments to the system. The scaled physical model is validated and calibrated through a HEC-HMS model to assure similitude of the system. Preliminary results of the physical model suggest that a 50-year storm event can be represented by a peak discharge of 2.2 X 10-3 cfs. When comparing the results to HEC-HMS, this equates to a flow relationship of approximately 1:160,000, which can be used to model other return periods. The completion of the Bell Canyon physical model can be used for educational instruction in the classroom, outreach in the community, and further research using the model as an accurate representation of the watershed present in the San Dimas Experimental Forest.

A Higher Order Iterative Method for Computing the Drazin Inverse

PubMed Central

Soleymani, F.; Stanimirović, Predrag S.

2013-01-01

A method with high convergence rate for finding approximate inverses of nonsingular matrices is suggested and established analytically. An extension of the introduced computational scheme to general square matrices is defined. The extended method could be used for finding the Drazin inverse. The application of the scheme on large sparse test matrices alongside the use in preconditioning of linear system of equations will be presented to clarify the contribution of the paper. PMID:24222747

Prediction-based association control scheme in dense femtocell networks

PubMed Central

Pham, Ngoc-Thai; Huynh, Thong; Hwang, Won-Joo; You, Ilsun; Choo, Kim-Kwang Raymond

2017-01-01

The deployment of large number of femtocell base stations allows us to extend the coverage and efficiently utilize resources in a low cost manner. However, the small cell size of femtocell networks can result in frequent handovers to the mobile user, and consequently throughput degradation. Thus, in this paper, we propose predictive association control schemes to improve the system’s effective throughput. Our design focuses on reducing handover frequency without impacting on throughput. The proposed schemes determine handover decisions that contribute most to the network throughput and are proper for distributed implementations. The simulation results show significant gains compared with existing methods in terms of handover frequency and network throughput perspective. PMID:28328992

On the convergence of nonconvex minimization methods for image recovery.

PubMed

Xiao, Jin; Ng, Michael Kwok-Po; Yang, Yu-Fei

2015-05-01

Nonconvex nonsmooth regularization method has been shown to be effective for restoring images with neat edges. Fast alternating minimization schemes have also been proposed and developed to solve the nonconvex nonsmooth minimization problem. The main contribution of this paper is to show the convergence of these alternating minimization schemes, based on the Kurdyka-Łojasiewicz property. In particular, we show that the iterates generated by the alternating minimization scheme, converges to a critical point of this nonconvex nonsmooth objective function. We also extend the analysis to nonconvex nonsmooth regularization model with box constraints, and obtain similar convergence results of the related minimization algorithm. Numerical examples are given to illustrate our convergence analysis.

Three-dimensional modelling of trace species in the Arctic lower stratosphere

NASA Technical Reports Server (NTRS)

Chipperfield, Martyn; Cariolle, Daniel; Simon, Pascal; Ramaroson, Richard

1994-01-01

A three-dimensional radiative-dynamical-chemical model has been developed and used to study some aspects of modeling the polar lower stratosphere. The model includes a comprehensive gas-phase chemistry scheme as well as a treatment of heterogeneous reactions occurring on the surface of polar stratospheric clouds. Tracer transport is treated by an accurate, nondispersive scheme with little diffusion suited to the representation of strong gradients. Results from a model simulation of early February 1990 are presented and used to illustrate the importance of the model transport scheme. The model simulation is also used to examine the potential for Arctic ozone destruction and the relative contributions of the chemical cycles responsible.

Policy Options to Reduce Fragmentation in the Pooling of Health Insurance Funds in Iran

PubMed Central

Bazyar, Mohammad; Rashidian, Arash; Kane, Sumit; Vaez Mahdavi, Mohammad Reza; Akbari Sari, Ali; Doshmangir, Leila

2016-01-01

There are fragmentations in Iran’s health insurance system. Multiple health insurance funds exist, without adequate provisions for transfer or redistribution of cross subsidy among them. Multiple risk pools, including several private secondary insurance schemes, have resulted in a tiered health insurance system with inequitable benefit packages for different segments of the population. Also fragmentation might have contributed to inefficiency in the health insurance systems, a low financial protection against healthcare expenditures for the insured persons, high coinsurance rates, a notable rate of insurance coverage duplication, low contribution of well-funded institutes with generous benefit package to the public health insurance schemes, underfunding and severe financial shortages for the public funds, and a lack of transparency and reliable data and statistics for policy-making. We have conducted a policy analysis study, including qualitative interviews of key informants and document analysis. As a result we introduce three policy options: keeping the existing structural fragmentations of social health insurance (SHI)schemes but implementing a comprehensive "policy integration" strategy; consolidation of existing health insurance funds and creating a single national health insurance scheme; and reducing fragmentation by merging minor well-resourced funds together and creating two or three large insurance funds under the umbrella of the existing organizations. These policy options with their advantages and disadvantages are explained in the paper. PMID:27239868

Policy Options to Reduce Fragmentation in the Pooling of Health Insurance Funds in Iran.

PubMed

Bazyar, Mohammad; Rashidian, Arash; Kane, Sumit; Vaez Mahdavi, Mohammad Reza; Akbari Sari, Ali; Doshmangir, Leila

2016-02-11

There are fragmentations in Iran's health insurance system. Multiple health insurance funds exist, without adequate provisions for transfer or redistribution of cross subsidy among them. Multiple risk pools, including several private secondary insurance schemes, have resulted in a tiered health insurance system with inequitable benefit packages for different segments of the population. Also fragmentation might have contributed to inefficiency in the health insurance systems, a low financial protection against healthcare expenditures for the insured persons, high coinsurance rates, a notable rate of insurance coverage duplication, low contribution of well-funded institutes with generous benefit package to the public health insurance schemes, underfunding and severe financial shortages for the public funds, and a lack of transparency and reliable data and statistics for policy-making. We have conducted a policy analysis study, including qualitative interviews of key informants and document analysis. As a result we introduce three policy options: keeping the existing structural fragmentations of social health insurance (SHI)schemes but implementing a comprehensive "policy integration" strategy; consolidation of existing health insurance funds and creating a single national health insurance scheme; and reducing fragmentation by merging minor well-resourced funds together and creating two or three large insurance funds under the umbrella of the existing organizations. These policy options with their advantages and disadvantages are explained in the paper. © 2016 by Kerman University of Medical Sciences.

A Target Coverage Scheduling Scheme Based on Genetic Algorithms in Directional Sensor Networks

PubMed Central

Gil, Joon-Min; Han, Youn-Hee

2011-01-01

As a promising tool for monitoring the physical world, directional sensor networks (DSNs) consisting of a large number of directional sensors are attracting increasing attention. As directional sensors in DSNs have limited battery power and restricted angles of sensing range, maximizing the network lifetime while monitoring all the targets in a given area remains a challenge. A major technique to conserve the energy of directional sensors is to use a node wake-up scheduling protocol by which some sensors remain active to provide sensing services, while the others are inactive to conserve their energy. In this paper, we first address a Maximum Set Covers for DSNs (MSCD) problem, which is known to be NP-complete, and present a greedy algorithm-based target coverage scheduling scheme that can solve this problem by heuristics. This scheme is used as a baseline for comparison. We then propose a target coverage scheduling scheme based on a genetic algorithm that can find the optimal cover sets to extend the network lifetime while monitoring all targets by the evolutionary global search technique. To verify and evaluate these schemes, we conducted simulations and showed that the schemes can contribute to extending the network lifetime. Simulation results indicated that the genetic algorithm-based scheduling scheme had better performance than the greedy algorithm-based scheme in terms of maximizing network lifetime. PMID:22319387

Sensitivity and Specificity of the World Health Organization Dengue Classification Schemes for Severe Dengue Assessment in Children in Rio de Janeiro

PubMed Central

Macedo, Gleicy A.; Gonin, Michelle Luiza C.; Pone, Sheila M.; Cruz, Oswaldo G.; Nobre, Flávio F.; Brasil, Patrícia

2014-01-01

Background The clinical definition of severe dengue fever remains a challenge for researchers in hyperendemic areas like Brazil. The ability of the traditional (1997) as well as the revised (2009) World Health Organization (WHO) dengue case classification schemes to detect severe dengue cases was evaluated in 267 children admitted to hospital with laboratory-confirmed dengue. Principal Findings Using the traditional scheme, 28.5% of patients could not be assigned to any category, while the revised scheme categorized all patients. Intensive therapeutic interventions were used as the reference standard to evaluate the ability of both the traditional and revised schemes to detect severe dengue cases. Analyses of the classified cases (n = 183) demonstrated that the revised scheme had better sensitivity (86.8%, P<0.001), while the traditional scheme had better specificity (93.4%, P<0.001) for the detection of severe forms of dengue. Conclusions/Significance This improved sensitivity of the revised scheme allows for better case capture and increased ICU admission, which may aid pediatricians in avoiding deaths due to severe dengue among children, but, in turn, it may also result in the misclassification of the patients' condition as severe, reflected in the observed lower positive predictive value (61.6%, P<0.001) when compared with the traditional scheme (82.6%, P<0.001). The inclusion of unusual dengue manifestations in the revised scheme has not shifted the emphasis from the most important aspects of dengue disease and the major factors contributing to fatality in this study: shock with consequent organ dysfunction. PMID:24777054

Sensitivity and specificity of the World Health Organization dengue classification schemes for severe dengue assessment in children in Rio de Janeiro.

PubMed

Macedo, Gleicy A; Gonin, Michelle Luiza C; Pone, Sheila M; Cruz, Oswaldo G; Nobre, Flávio F; Brasil, Patrícia

2014-01-01

The clinical definition of severe dengue fever remains a challenge for researchers in hyperendemic areas like Brazil. The ability of the traditional (1997) as well as the revised (2009) World Health Organization (WHO) dengue case classification schemes to detect severe dengue cases was evaluated in 267 children admitted to hospital with laboratory-confirmed dengue. Using the traditional scheme, 28.5% of patients could not be assigned to any category, while the revised scheme categorized all patients. Intensive therapeutic interventions were used as the reference standard to evaluate the ability of both the traditional and revised schemes to detect severe dengue cases. Analyses of the classified cases (n = 183) demonstrated that the revised scheme had better sensitivity (86.8%, P<0.001), while the traditional scheme had better specificity (93.4%, P<0.001) for the detection of severe forms of dengue. This improved sensitivity of the revised scheme allows for better case capture and increased ICU admission, which may aid pediatricians in avoiding deaths due to severe dengue among children, but, in turn, it may also result in the misclassification of the patients' condition as severe, reflected in the observed lower positive predictive value (61.6%, P<0.001) when compared with the traditional scheme (82.6%, P<0.001). The inclusion of unusual dengue manifestations in the revised scheme has not shifted the emphasis from the most important aspects of dengue disease and the major factors contributing to fatality in this study: shock with consequent organ dysfunction.

Power Smoothing of a Variable-Speed Wind Turbine Generator in Association With the Rotor-Speed-Dependent Gain

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kim, Yeonhee; Kang, Moses; Muljadi, Eduard

This paper proposes a power-smoothing scheme for a variable-speed wind turbine generator (WTG) that can smooth out the WTG's fluctuating power caused by varying wind speeds, and thereby keep the system frequency within a narrow range. The proposed scheme employs an additional loop based on the system frequency deviation that operates in conjunction with the maximum power point tracking (MPPT) control loop. Unlike the conventional, fixed-gain scheme, its control gain is modified with the rotor speed. In the proposed scheme, the control gain is determined by considering the ratio of the output of the additional loop to that of themore » MPPT loop. To improve the contribution of the scheme toward maintaining the frequency while ensuring the stable operation of WTGs, in the low rotor speed region, the ratio is set to be proportional to the rotor speed; in the high rotor speed region, the ratio remains constant. The performance of the proposed scheme is investigated under varying wind conditions for the IEEE 14-bus system. The simulation results demonstrate that the scheme successfully operates regardless of the output power fluctuation of a WTG by adjusting the gain with the rotor speed, and thereby improves the frequency-regulating capability of a WTG.« less

A comparison of two multi-variable integrator windup protection schemes

NASA Technical Reports Server (NTRS)

Mattern, Duane

1993-01-01

Two methods are examined for limit and integrator wind-up protection for multi-input, multi-output linear controllers subject to actuator constraints. The methods begin with an existing linear controller that satisfies the specifications for the nominal, small perturbation, linear model of the plant. The controllers are formulated to include an additional contribution to the state derivative calculations. The first method to be examined is the multi-variable version of the single-input, single-output, high gain, Conventional Anti-Windup (CAW) scheme. Except for the actuator limits, the CAW scheme is linear. The second scheme to be examined, denoted the Modified Anti-Windup (MAW) scheme, uses a scalar to modify the magnitude of the controller output vector while maintaining the vector direction. The calculation of the scalar modifier is a nonlinear function of the controller outputs and the actuator limits. In both cases the constrained actuator is tracked. These two integrator windup protection methods are demonstrated on a turbofan engine control system with five measurements, four control variables, and four actuators. The closed-loop responses of the two schemes are compared and contrasted during limit operation. The issue of maintaining the direction of the controller output vector using the Modified Anti-Windup scheme is discussed and the advantages and disadvantages of both of the IWP methods are presented.

The incidence of health financing in South Africa: findings from a recent data set.

PubMed

Ataguba, John E; McIntyre, Di

2018-01-01

There is an international call for countries to ensure universal health coverage. This call has been embraced in South Africa (SA) in the form of a National Health Insurance (NHI). This is expected to be financed through general tax revenue with the possibility of additional earmarked taxes including a surcharge on personal income and/or a payroll tax for employers. Currently, health services are financed in SA through allocations from general tax revenue, direct out-of-pocket payments, and contributions to medical scheme. This paper uses the most recent data set to assess the progressivity of each health financing mechanism and overall financing system in SA. Applying standard and innovative methodologies for assessing progressivity, the study finds that general taxes and medical scheme contributions remain progressive, and direct out-of-pocket payments and indirect taxes are regressive. However, private health insurance contributions, across only the insured, are regressive. The policy implications of these findings are discussed in the context of the NHI.

Sele coastal plain flood risk due to wave storm and river flow interaction

NASA Astrophysics Data System (ADS)

Benassai, Guido; Aucelli, Pietro; Di Paola, Gianluigi; Della Morte, Renata; Cozzolino, Luca; Rizzo, Angela

2016-04-01

Wind waves, elevated water levels and river discharge can cause flooding in low-lying coastal areas, where the water level is the interaction between wave storm elevated water levels and river flow interaction. The factors driving the potential flood risk include weather conditions, river water stage and storm surge. These data are required to obtain inputs to run the hydrological model used to evaluate the water surface level during ordinary and extreme events regarding both the fluvial overflow and storm surge at the river mouth. In this paper we studied the interaction between the sea level variation and the river hydraulics in order to assess the location of the river floods in the Sele coastal plain. The wave data were acquired from the wave buoy of Ponza, while the water level data needed to assess the sea level variation were recorded by the tide gauge of Salerno. The water stages, river discharges and rating curves for Sele river were provided by Italian Hydrographic Service (Servizio Idrografico e Mareografico Nazionale, SIMN).We used the dataset of Albanella station (40°29'34.30"N, 15°00'44.30"E), located around 7 km from the river mouth. The extreme river discharges were evaluated through the Weibull equation, which were associated with their return period (TR). The steady state river water levels were evaluated through HEC-RAS 4.0 model, developed by Hydrologic Engineering Center (HEC) of the United States Army Corps of Engineers Hydrologic Engineering Center (USACE,2006). It is a well-known 1D model that computes water surface elevation (WSE) and velocity at discrete cross-sections by solving continuity, energy and flow resistance (e.g., Manning) equation. Data requirements for HEC-RAS include topographic information in the form of a series of cross-sections, friction parameter in the form of Manning's n values across each cross-section, and flow data including flow rates, flow change locations, and boundary conditions. For a steady state sub-critical simulation, the boundary condition is a known downstream WSE, in this case the elevated water level due to wave setup, wind setup and inverted barometer, while the upstream boundary condition consisted in WSE corresponding to river discharges associated to different return periods. The results of the simulations evidence, for the last 10 kilometers of the river, the burst of critical inundation scenarios even with moderate flow discharge, if associated with concurrent storm surge which increase the water level at the river mouth, obstructing normal flow discharge.

Simulations of flow and prediction of sediment movement in Wymans Run, Cochranton Borough, Crawford County, Pennsylvania

USGS Publications Warehouse

Hittle, Elizabeth

2011-01-01

In small watersheds, runoff entering local waterways from large storms can cause rapid and profound changes in the streambed that can contribute to flooding. Wymans Run, a small stream in Cochranton Borough, Crawford County, experienced a large rain event in June 2008 that caused sediment to be deposited at a bridge. A hydrodynamic model, Flow and Sediment Transport and Morphological Evolution of Channels (FaSTMECH), which is incorporated into the U.S. Geological Survey Multi-Dimensional Surface-Water Modeling System (MD_SWMS) was constructed to predict boundary shear stress and velocity in Wymans Run using data from the June 2008 event. Shear stress and velocity values can be used to indicate areas of a stream where sediment, transported downstream, can be deposited on the streambed. Because of the short duration of the June 2008 rain event, streamflow was not directly measured but was estimated using U.S. Army Corps of Engineers one-dimensional Hydrologic Engineering Centers River Analysis System (HEC-RAS). Scenarios to examine possible engineering solutions to decrease the amount of sediment at the bridge, including bridge expansion, channel expansion, and dredging upstream from the bridge, were simulated using the FaSTMECH model. Each scenario was evaluated for potential effects on water-surface elevation, boundary shear stress, and velocity.

Maternity Charities, the Edinburgh Maternity Scheme and the Medicalisation of Childbirth, 1900–1925

PubMed Central

Nuttall, Alison

2011-01-01

Summary Increased medical involvement in maternal welfare has been linked with the introduction of local authority administered schemes associated with government concern for women's health that reached a peak during the First World War. Although local studies have noted the work of philanthropic groups, the implication has been that their contribution to the medicalisation of childbirth was small. This article uses analysis of the personal health records of users of Edinburgh's maternity charities to argue that the process of medicalisation was begun by these charities, and preceded the introduction of the Edinburgh Maternity and Child Welfare Scheme in 1917. However, whilst it is argued that initially the Scheme had limited impact, the article concludes that its funding and stability offered the opportunity for more dynamic management of abnormal pregnancies. Thus this encouraged a gradual shift in attitude to birth from an essentially physiological event to a potentially pathological incident.

Contribution of the Recent AUSM Schemes to the Overflow Code: Implementation and Validation

NASA Technical Reports Server (NTRS)

Liou, Meng-Sing; Buning, Pieter G.

2000-01-01

We shall present results of a recent collaborative effort between the authors attempting to implement the numerical flux scheme, AUSM+ and its new developments, into a widely used NASA code, OVERFLOW. This paper is intended to give a thorough and systematic documentation about the solutions of default test cases using the AUSNI+ scheme. Hence we will address various aspects of numerical solutions, such as accuracy, convergence rate, and effects of turbulence models, over a variety of geometries, speed regimes. We will briefly describe the numerical schemes employed in the calculations, including the capability of solving for low-speed flows and multiphase flows by employing the concept of numerical speed of sound. As a bonus, this low Mach number formulations also enhances convergence to steady solutions for flows even at transonic speed. Calculations for complex 3D turbulent flows were performed with several turbulence models and the results display excellent agreements with measured data.

Oral health finance and expenditure in South Africa.

PubMed

Naidoo, L C; Stephen, L X

1997-12-01

The objective of this paper was to examine the cost of oral health in South Africa over the past decade Particular emphasis was placed on the contribution made by medical schemes which is the main source of private health care funding. Some of the problems facing this huge industry were also briefly explored. Primary aggregate data on oral health expenditure were obtained from the Department of Health, Pretoria and from the offices of the Registrar of Medical Schemes, Pretoria. The results show that in 1994, 4.7 per cent of the total health care budget was allocated to oral health. Of this amount, 14.2 per cent came from the state, 71.9 per cent from medical schemes and the remainder calculated to be from direct out-of-pocket payments. Furthermore, real expenditure for oral health by medical schemes grew robustly and almost continuously from 1984 through to 1994, generally outstripping medical inflation.

Fair ranking of researchers and research teams

PubMed Central

2018-01-01

The main drawback of ranking of researchers by the number of papers, citations or by the Hirsch index is ignoring the problem of distributing authorship among authors in multi-author publications. So far, the single-author or multi-author publications contribute to the publication record of a researcher equally. This full counting scheme is apparently unfair and causes unjust disproportions, in particular, if ranked researchers have distinctly different collaboration profiles. These disproportions are removed by less common fractional or authorship-weighted counting schemes, which can distribute the authorship credit more properly and suppress a tendency to unjustified inflation of co-authors. The urgent need of widely adopting a fair ranking scheme in practise is exemplified by analysing citation profiles of several highly-cited astronomers and astrophysicists. While the full counting scheme often leads to completely incorrect and misleading ranking, the fractional or authorship-weighted schemes are more accurate and applicable to ranking of researchers as well as research teams. In addition, they suppress differences in ranking among scientific disciplines. These more appropriate schemes should urgently be adopted by scientific publication databases as the Web of Science (Thomson Reuters) or the Scopus (Elsevier). PMID:29621316

Renormalization scheme and gauge (in)dependence of the generalized Crewther relation: what are the real grounds of the β-factorization property?

NASA Astrophysics Data System (ADS)

Garkusha, A. V.; Kataev, A. L.; Molokoedov, V. S.

2018-02-01

The problem of scheme and gauge dependence of the factorization property of the renormalization group β-function in the SU( N c ) QCD generalized Crewther relation (GCR), which connects the flavor non-singlet contributions to the Adler and Bjorken polarized sum rule functions, is investigated at the O({a}_s^4) level of perturbation theory. It is known that in the gauge-invariant renormalization \\overline{MS} -scheme this property holds in the QCD GCR at least at this order. To study whether this factorization property is true in all gauge-invariant schemes, we consider the MS-like schemes in QCD and the QED-limit of the GCR in the \\overline{MS} -scheme and in two other gauge-independent subtraction schemes, namely in the momentum MOM and the on-shell OS schemes. In these schemes we confirm the existence of the β-function factorization in the QCD and QED variants of the GCR. The problem of the possible β-factorization in the gauge-dependent renormalization schemes in QCD is studied. To investigate this problem we consider the gauge non-invariant mMOM and MOMgggg-schemes. We demonstrate that in the mMOM scheme at the O({a}_s^3) level the β-factorization is valid for three values of the gauge parameter ξ only, namely for ξ = -3 , -1 and ξ = 0. In the O({a}_s^4) order of PT it remains valid only for case of the Landau gauge ξ = 0. The consideration of these two gauge-dependent schemes for the QCD GCR allows us to conclude that the factorization of RG β-function will always be implemented in any MOM-like renormalization schemes with linear covariant gauge at ξ = 0 and ξ = -3 at the O({a}_s^3) approximation. It is demonstrated that if factorization property for the MS-like schemes is true in all orders of PT, as theoretically indicated in the several works on the subject, then the factorization will also occur in the arbitrary MOM-like scheme in the Landau gauge in all orders of perturbation theory as well.

A TorPath to TorCoin: Proof-of-Bandwidth Altcoins for Compensating Relays

DTIC Science & Technology

2014-07-18

incentive scheme for Tor relying on two novel concepts. We introduce TorCoin, an “altcoin” that uses the Bitcoin protocol to re- ward relays for...or altcoin, based on the Bitcoin protocol [8]. Unlike Bitcoin , its proof-of-work scheme is based on bandwidth rather than computation. To “mine” a...concepts. We introduce TorCoin, an altcoin" that uses the Bitcoin protocol to re- ward relays for contributing bandwidth. Relays mine" TorCoins, then

Costs and effects of the Tanzanian national voucher scheme for insecticide-treated nets

PubMed Central

Mulligan, Jo-Ann; Yukich, Joshua; Hanson, Kara

2008-01-01

Background The cost-effectiveness of insecticide-treated nets (ITNs) in reducing morbidity and mortality is well established. International focus has now moved on to how best to scale up coverage and what financing mechanisms might be used to achieve this. The approach in Tanzania has been to deliver a targeted subsidy for those most vulnerable to the effects of malaria while at the same time providing support to the development of the commercial ITN distribution system. In October 2004, with funds from the Global Fund to Fight AIDS Tuberculosis and Malaria, the government launched the Tanzania National Voucher Scheme (TNVS), a nationwide discounted voucher scheme for ITNs for pregnant women and their infants. This paper analyses the costs and effects of the scheme and compares it with other approaches to distribution. Methods Economic costs were estimated using the ingredients approach whereby all resources required in the delivery of the intervention (including the user contribution) are quantified and valued. Effects were measured in terms of number of vouchers used (and therefore nets delivered) and treated nets years. Estimates were also made for the cost per malaria case and death averted. Results and Conclusion The total financial cost of the programme represents around 5% of the Ministry of Health's total budget. The average economic cost of delivering an ITN using the voucher scheme, including the user contribution, was $7.57. The cost-effectiveness results are within the benchmarks set by other malaria prevention studies. The Government of Tanzania's approach to scaling up ITNs uses both the public and private sectors in order to achieve and sustain the level of coverage required to meet the Abuja targets. The results presented here suggest that the TNVS is a cost-effective strategy for delivering subsidized ITNs to targeted vulnerable groups. PMID:18279509