ARES I-X Launch

NASA Image and Video Library

2009-10-27

NASA's Ares I-X rocket is seen through the windows of Firing Room One of teh Launch Control Center (LCC) at the Kennedy Space Center as it launches from pad 39b in Cape Canaveral, Fla., Wednesday, Oct. 28, 2009. The flight test will provide NASA with an early opportunity to test and prove flight characteristics, hardware, facilities and ground operations associated with the Ares I. Photo Credit: (NASA/Bill Ingalls)

KSC-2013-1783

NASA Image and Video Library

2013-03-05

CAPE CANAVERAL, Fla. – A telemetry antenna and tracker camera is attached to the roof of the Launch Control Center, or LCC, in Launch Complex 39 at NASA's Kennedy Space Center in Florida. This antenna and camera system is the first of three to be installed on the LCC roof for the Radio Frequency and Telemetry Station RFTS, which will be used to monitor radio frequency communications from a launch vehicle at Launch Pad 39A or B as well as provide radio frequency relay for a launch vehicle in the Vehicle Assembly Building. The RFTS replaces the shuttle-era communications and tracking labs at Kennedy. The modern RFTS checkout station is designed to primarily support NASA's Space Launch System, or SLS, and Orion spacecraft, but can support multi-user radio frequency tests as the space center transitions to support a variety of rockets and spacecraft. For more information on the modernization efforts at Kennedy, visit the Ground Systems Development and Operations, or GSDO, website at http://go.nasa.gov/groundsystems. Photo credit: NASA/Jim Grossmann

STS-135 Atlantis Launch

NASA Image and Video Library

2011-07-07

NASA Photographer Kim Shiflett, left, and Videographer Glenn Benson capture a group photo of the launch team in Firing Room Four of the NASA Kennedy Space Center Launch Control Center (LCC) shortly after the space shuttle Atlantis, STS-135, launched on Friday, July 8, 2011, in Cape Canaveral, Fla. The launch of Atlantis is the final flight of the shuttle program, a 12-day mission to the International Space Station. Photo Credit: (NASA/Bill Ingalls)

ARES I-X Launch Prep

NASA Image and Video Library

2009-10-26

NASA Ares I-X Launch Director Ed Mango monitors the launch countdown from Firing Room One of the Launch Control Center (LCC) at the Kennedy Space Center during the planned launch of the Ares I-X rocket from pad 39b at the Kennedy Space Center in Cape Canaveral, Fla., Tuesday, Oct. 27, 2009. The flight test of Ares I-X will provide NASA with an early opportunity to test and prove flight characteristics, hardware, facilities and ground operations associated with the Ares I. Photo Credit: (NASA/Bill Ingalls)

STS-135 Atlantis Launch

NASA Image and Video Library

2011-07-07

NASA Administrator Charles Bolden speaks to visitors at the NASA Kennedy Space Center Banana Creek viewing site prior to going to the Launch Control Center (LCC) for the planned launch of the space shuttle Atlantis from pad 39A on Friday, July 8, 2011, in Cape Canaveral, Fla. The launch of Atlantis, STS-135, is the final flight of the shuttle program, a 12-day mission to the International Space Station. Photo Credit: (NASA/Bill Ingalls)

KSC-2013-1782

NASA Image and Video Library

2013-03-05

CAPE CANAVERAL, Fla. – With the help of a crane, a worker helps guide a parabolic telemetry antenna and tracker camera to the roof of the Launch Control Center, or LCC, in Launch Complex 39 at NASA's Kennedy Space Center in Florida. This antenna and camera system is the first of three that will be installed on the LCC roof for the Radio Frequency and Telemetry Station RFTS, which will be used to monitor radio frequency communications from a launch vehicle at Launch Pad 39A or B as well as provide radio frequency relay for a launch vehicle in the Vehicle Assembly Building. The RFTS replaces the shuttle-era communications and tracking labs at Kennedy. The modern RFTS checkout station is designed to primarily support NASA's Space Launch System, or SLS, and Orion spacecraft, but can support multi-user radio frequency tests as the space center transitions to support a variety of rockets and spacecraft. For more information on the modernization efforts at Kennedy, visit the Ground Systems Development and Operations, or GSDO, website at http://go.nasa.gov/groundsystems. Photo credit: NASA/Jim Grossmann

Mass Analyzers Facilitate Research on Addiction

NASA Technical Reports Server (NTRS)

2012-01-01

The famous go/no go command for Space Shuttle launches comes from a place called the Firing Room. Located at Kennedy Space Center in the Launch Control Center (LCC), there are actually four Firing Rooms that take up most of the third floor of the LCC. These rooms comprise the nerve center for Space Shuttle launch and processing. Test engineers in the Firing Rooms operate the Launch Processing System (LPS), which is a highly automated, computer-controlled system for assembly, checkout, and launch of the Space Shuttle. LPS monitors thousands of measurements on the Space Shuttle and its ground support equipment, compares them to predefined tolerance levels, and then displays values that are out of tolerance. Firing Room operators view the data and send commands about everything from propellant levels inside the external tank to temperatures inside the crew compartment. In many cases, LPS will automatically react to abnormal conditions and perform related functions without test engineer intervention; however, firing room engineers continue to look at each and every happening to ensure a safe launch. Some of the systems monitored during launch operations include electrical, cooling, communications, and computers. One of the thousands of measurements derived from these systems is the amount of hydrogen and oxygen inside the shuttle during launch.

Preparation of Degradable Biological Carrier With LCC and its Application in Culture of Hepatocytes

NASA Astrophysics Data System (ADS)

Zhao, H. K.; Chen, X. K.; Wu, H. F.; Li, J. L.; Xie, Y. M.

2018-05-01

The purpose of this article is to extract lignin-carbohydrate complexes (LCC) with poplar as raw material, which was used to prepare bio-carrier by freeze-drying method. The chemical properties and morphological of LCC porous biological carriers were analyzed by GPC, FT-IR, scanning electron microscopy (SEM) and optical microscopy. The FT-IR spectrum results indicated that LCC which are composed of lignin and polysaccharide, with a typical LCC structure. Galactose have a specific ability to recognize liver cells owing to the presence of receptors on hepatocytes. Cell counting results showed that the cells increases fastest while the proliferation rate of the liver cell in LCC is obviously higher than that of control group. These results indicated that poplar LCC is very biocompatible, in which it might be a great potential biological carrier material for human hepatocyte culture.

ARES I-X Launch

NASA Image and Video Library

2009-10-27

NASA Ares I-X Launch Director Ed Mango, 3rd from left, along with other mission managers watches the launch of the Ares I-X rocket from Firing Room One of the Launch Control Center (LCC) at the Kennedy Space Center in Cape Canaveral, Fla., Wednesday, Oct. 28, 2009. The flight test of Ares I-X will provide NASA with an early opportunity to test and prove flight characteristics, hardware, facilities and ground operations associated with the Ares I. Photo Credit: (NASA/Bill Ingalls)

Diosmin/essential oil combination for dermal photo-protection using a lipoid colloidal carrier.

PubMed

Kamel, Rabab; Abbas, Haidy; Fayez, Ahmed

2017-05-01

Solar irradiation induces skin inflammatory processes causing deleterious effects like premature ageing. In this study, the designed lipoid colloidal carrier (LCC) was loaded with Diosmin in combination with different essential oils, to be used as a topical photo-protective preparation. To investigate the ability of the essential oils to potentiate Diosmin effects, the Diosmin/essential oil-loaded LCCs (LCC2, LCC3 and LCC4) were compared to the Diosmin-loaded LCC (LCC1). The incorporated essential oils were those of Rosmarinus officinalis, Zingiber officinale or Vitis vinifera in LCC2, LCC3 and LCC4, respectively. All the LCCs had particle size (PS) values ranging from 121.1 to 144.3nm with uniform distribution and, zeta potential (Z) values around 30mV. Also, they all had high drug encapsulation efficiencies. LCC1 had the lowest anti-oxidant and in-vitro sun-blocking effect (p<0.05). In-vivo photo-protective studies showed that all the formulated LCCs had a skin protective effect when compared to the positive control (p<0.05); however LCC1 had the lowest anti-erythemal and anti-wrinkling effect. Histological studies proved the efficacy of the designed LCCs as skin anti-photoageing, with LCC1 showing the lowest anti-inflammatory and anti-wrinkling effect, while LCC2 had the highest anti-wrinkling effect. These results indicated that the suggested Diosmin/essential oil combinations improved the anti-oxidant, sun-blocking and anti-photoageing effects of Diosmin. After one year of storage, the LCCs showed satisfactory physical stability. This study presents the designed LCCs as safe and effective nano-structured dermal care products containing 'all-natural' components. Copyright © 2017 Elsevier B.V. All rights reserved.

Positive tropical marine low-cloud cover feedback inferred from cloud-controlling factors

DOE PAGES

Qu, Xin; Hall, Alex; Klein, Stephen A.; ...

2015-09-28

Differences in simulations of tropical marine low-cloud cover (LCC) feedback are sources of significant spread in temperature responses of climate models to anthropogenic forcing. Here we show that in models the feedback is mainly driven by three large-scale changes—a strengthening tropical inversion, increasing surface latent heat flux, and an increasing vertical moisture gradient. Variations in the LCC response to these changes alone account for most of the spread in model-projected 21st century LCC changes. A methodology is devised to constrain the LCC response observationally using sea surface temperature (SST) as a surrogate for the latent heat flux and moisture gradient.more » In models where the current climate's LCC sensitivities to inversion strength and SST variations are consistent with observed, LCC decreases systematically, which would increase absorption of solar radiation. These results support a positive LCC feedback. Finally, correcting biases in the sensitivities will be an important step toward more credible simulation of cloud feedbacks.« less

The Irpinia Seismic Network (ISN): a new Monitoring Infrastructure for Seismic Alert Management in Campania Region, Southern Italy

NASA Astrophysics Data System (ADS)

Iannaccone, G.; Satriano, C.; Weber, E.; Cantore, L.; Corciulo, M.; Romano, L.; Martino, C.; Dicrosta, M.; Zollo, A.

2005-12-01

The Irpinia Seismic Network is an high dynamics, high density seismographic network under development in the Southern Apenninic chain. It is deployed in the area stroken by several destructive earthquakes during last centuries. In its final configuration the network will consist of more than fourty high dynamic seismic stations subdivided in physical subnetworks inter-connected by a robust data transmission system. The system is being designed with two primary targets: -Monitoring and analysis of background seismic activity produced by the active fault system which is the cause for large earthquakes in the past, included the 1980, Irpinia earthquake (Ms=6.9) - Development and experimentation of a prototype system for seismic early and post-event warning to be used for protecting public infrastructures and buildings of strategic relevance of the Regione Campania The seismic network will be completed in two stages: 1 - Deployment of 30 seismic stations along the Campania-Lucania Apenninic chain (to date almost completed) 2 - Setting up radio communication system for data transmission. Installation of 12 additional seismic stations (end of year 2006) To ensure an high dynamic recording range each site is equipped with two type of sensors: 30 force-balance accelerometer (model Guralp CMG5-T) and a velocimeter. In particular, 25 sites with short period three components instrument (model Geotech S13-J) and 5 with broad-band sensor (Nanometrics Trillium, with frequency response in the 0.033-50 Hz band). The used data logger is the Osiris-6 model produced by Agecodagis whose main features are: six channels, O/N 24 bit A/D converter, ARM processor with embedded Linux and open source software, two PCMCIA slots (used for two 5GB microdrive or one disk and wi-fi card), Ethernet, wi-fi and serial communication, low power cosumption (~1 W). Power is ensured by two 120 W solar panels and two 130 Ah gel batteries. Each recording site is equipped with a control/alarm system through a Programmable Logic Controller-GSM modem connected to several ambient sensors (forcing, battery control, fire, temperature) to remotely control the site status. Data are locally stored on the 5GB disk and continuously transmitted by the SeedLink protocol through a point-to-point wireless LAN bridge to interconnection nodes (Local Control Center, LCC). At LCC sites an Earthworm system runs on a dedicated computer to manage the data stream acquired by stations directly connected to LCC. The real time analysis system performs event detection and location based on triggers coming from data loggers and parametric information provided by the other LCCs. At present, data transmission among LCC is performed via commercial ADSL. Once an event is detected and located, the system performs automatic magnitude and focal mechanism estimation. The results of this analysis are used to build a local event Data Base (DB) and, at the same time, they are sent to other LCC and to the network operating room located in Naples (RISSC). At RISSC center the network data are managed by a system gathering parametric data from all the LCC and performing real time analysis based on which it is decided whether or not issuing a seismic alert. Future plans for network operation involve the installation of additional 12 stations, and the upgrading of data transmission system to a proprietary radio link with SDH technology. The project is financial supported by the Campania Regional Department of Civil Protection.

The multi-disciplinary design study: A life cycle cost algorithm

NASA Technical Reports Server (NTRS)

Harding, R. R.; Pichi, F. J.

1988-01-01

The approach and results of a Life Cycle Cost (LCC) analysis of the Space Station Solar Dynamic Power Subsystem (SDPS) including gimbal pointing and power output performance are documented. The Multi-Discipline Design Tool (MDDT) computer program developed during the 1986 study has been modified to include the design, performance, and cost algorithms for the SDPS as described. As with the Space Station structural and control subsystems, the LCC of the SDPS can be computed within the MDDT program as a function of the engineering design variables. Two simple examples of MDDT's capability to evaluate cost sensitivity and design based on LCC are included. MDDT was designed to accept NASA's IMAT computer program data as input so that IMAT's detailed structural and controls design capability can be assessed with expected system LCC as computed by MDDT. No changes to IMAT were required. Detailed knowledge of IMAT is not required to perform the LCC analyses as the interface with IMAT is noninteractive.

ASCANS Saturn V & LCC Tour

NASA Image and Video Library

2014-03-03

CAPE CANAVERAL, Fla. – NASA astronaut candidate Andrew Morgan surveys the mission plaques on the wall of the Launch Control Center at Kennedy Space Center in Florida during a daylong set of briefings and tours of different facilities at NASA's primary launch center. The astronaut class of 2013 was selected by NASA after an extensive year-and-a-half search. The new group will help the agency push the boundaries of exploration and travel to new destinations in the solar system. To learn more about the astronaut class of 2013, visit: http://www.nasa.gov/astronauts/2013astroclass.html Photo credit: NASA/Kim Shiflett

ARES I-X Launch Prep

NASA Image and Video Library

2009-10-26

NASA Ares I-X Assistant Launch Director Pete Nickolenko, left, and NASA Ares I-X Launch Director Ed Mango monitor the launch countdown from Firing Room One of the Launch Control Center (LCC) at the Kennedy Space Center during the planned launch of the Ares I-X rocket from pad 39b at the Kennedy Space Center in Cape Canaveral, Fla., Tuesday, Oct. 27, 2009. The flight test of Ares I-X will provide NASA with an early opportunity to test and prove flight characteristics, hardware, facilities and ground operations associated with the Ares I. Photo Credit: (NASA/Bill Ingalls)

ARES I-X Launch

NASA Image and Video Library

2009-10-27

NASA Ares I-X Launch Director Ed Mango, left, laughs as NASA Ares I-X Assistant Launch Director Pete Nickolenko looks out the window of Firing Room One of the Launch Control Center (LCC) at the Kennedy Space Center prior to the launch of the Ares I-X rocket from pad 39b at the Kennedy Space Center in Cape Canaveral, Fla., Wednesday, Oct. 28, 2009. The flight test of Ares I-X will provide NASA with an early opportunity to test and prove flight characteristics, hardware, facilities and ground operations associated with the Ares I. Photo Credit: (NASA/Bill Ingalls)

KSC-2010-5737

NASA Image and Video Library

2010-11-24

CAPE CANAVERAL, Fla. -- The finishing touches of the Propellants North Administrative and Maintenance Facility begin to take place at NASA's Kennedy Space Center in Florida. Inside the green facility is window glazing and framing from the iconic firing rooms of Kennedy's Launch Control Center (LCC). The windows are set at the same orientation and angle as they were in the LCC, looking out toward Launch Complex 39. The facility also features sustainable flooring made of polished concrete and laminated bamboo, as well as a high-efficiency roof and walls. This is the facility's two-story administrative building, which will house managers, mechanics and technicians who fuel spacecraft at Kennedy. Next door is a single-story shop that will be used to store cryogenic fuel transfer equipment. The facility is striving to qualify for the U.S. Green Building Council’s Leadership in Energy and Environmental Design (LEED) Platinum certification, which is the highest of LEED ratings. The facility, set to be complete in December 2010, was designed for NASA by Jones Edmunds and Associates. H. W. Davis Construction is the construction contractor. Photo credit: NASA/Frankie Martin

KENNEDY SPACE CENTER, FLA. - In the Launch Control Center, officials monitor the “Mode VII” emergency landing simulation being conducted at Kennedy Space Center and managed and directed from the LCC. From left are Dr. Luis Moreno and Dr. David Reed, with Bionetics Life Sciences, and Dr. Philip Scarpa, with the KSC Safety, Occupational Health and Environment Division. The purpose of the Mode VII is to exercise emergency preparedness personnel, equipment and facilities in rescuing astronauts from a downed orbiter and providing immediate medical attention. This simulation presents an orbiter that has crashed short of the Shuttle Landing Facility in a wooded area 2-1/2 miles south of Runway 33. Emergency crews are responding to the volunteer “astronauts” who are simulating various injuries inside the crew compartment mock-up. Rescuers must remove the crew, provide triage and transport to hospitals those who need further treatment. Local hospitals are participating in the exercise.

NASA Image and Video Library

2004-02-18

KENNEDY SPACE CENTER, FLA. - In the Launch Control Center, officials monitor the “Mode VII” emergency landing simulation being conducted at Kennedy Space Center and managed and directed from the LCC. From left are Dr. Luis Moreno and Dr. David Reed, with Bionetics Life Sciences, and Dr. Philip Scarpa, with the KSC Safety, Occupational Health and Environment Division. The purpose of the Mode VII is to exercise emergency preparedness personnel, equipment and facilities in rescuing astronauts from a downed orbiter and providing immediate medical attention. This simulation presents an orbiter that has crashed short of the Shuttle Landing Facility in a wooded area 2-1/2 miles south of Runway 33. Emergency crews are responding to the volunteer “astronauts” who are simulating various injuries inside the crew compartment mock-up. Rescuers must remove the crew, provide triage and transport to hospitals those who need further treatment. Local hospitals are participating in the exercise.

Concepts for Life Cycle Cost Control Required to Achieve Space Transportation Affordability and Sustainability

NASA Technical Reports Server (NTRS)

Rhodes, Russel E.; Zapata, Edgar; Levack, Daniel J. H.; Robinson, John W.; Donahue, Benjamin B.

2009-01-01

Cost control must be implemented through the establishment of requirements and controlled continually by managing to these requirements. Cost control of the non-recurring side of life cycle cost has traditionally been implemented in both commercial and government programs. The government uses the budget process to implement this control. The commercial approach is to use a similar process of allocating the non-recurring cost to major elements of the program. This type of control generally manages through a work breakdown structure (WBS) by defining the major elements of the program. If the cost control is to be applied across the entire program life cycle cost (LCC), the approach must be addressed very differently. A functional breakdown structure (FBS) is defined and recommended. Use of a FBS provides the visibifity to allow the choice of an integrated solution reducing the cost of providing many different elements of like function. The different functional solutions that drive the hardware logistics, quantity of documentation, operational labor, reliability and maintainability balance, and total integration of the entire system from DDT&E through the life of the program must be fully defined, compared, and final decisions made among these competing solutions. The major drivers of recurring cost have been identified and are presented and discussed. The LCC requirements must be established and flowed down to provide control of LCC. This LCC control will require a structured rigid process similar to the one traditionally used to control weight/performance for space transportation systems throughout the entire program. It has been demonstrated over the last 30 years that without a firm requirement and methodically structured cost control, it is unlikely that affordable and sustainable space transportation system LCC will be achieved.

ASCANS Saturn V & LCC Tour

NASA Image and Video Library

2014-03-03

CAPE CANAVERAL, Fla. – NASA astronaut candidates Jessica Meir, Tyler Nick Hague and Nicole Mann listen to a discussion about firing rooms inside the Launch Control Center at Kennedy Space Center in Florida during a daylong set of briefings and tours of different facilities at NASA's primary launch center. The astronaut class of 2013 was selected by NASA after an extensive year-and-a-half search. The new group will help the agency push the boundaries of exploration and travel to new destinations in the solar system. To learn more about the astronaut class of 2013, visit: http://www.nasa.gov/astronauts/2013astroclass.html Photo credit: NASA/Kim Shiflett

A Comparative Analysis on Assessment of Land Carrying Capacity with Ecological Footprint Analysis and Index System Method

PubMed Central

Qian, Yao; Tang, Lina; Qiu, Quanyi; Xu, Tong; Liao, Jiangfu

2015-01-01

Land carrying capacity (LCC) explains whether the local land resources are effectively used to support economic activities and/or human population. LCC can be evaluated commonly with two approaches, namely ecological footprint analysis (EFA) and the index system method (ISM). EFA is helpful to investigate the effects of different land categories whereas ISM can be used to evaluate the contributions of social, environmental, and economic factors. Here we compared the two LCC-evaluation approaches with data collected from Xiamen City, a typical region where rapid economic growth and urbanization are found in China. The results show that LCC assessments with EFA and ISM not only complement each other but also are mutually supportive. Both assessments suggest that decreases in arable land and increasingly high energy consumption have major negative effects on LCC and threaten sustainable development for Xiamen City. It is important for the local policy makers, planners and designers to reduce ecological deficits by controlling fossil energy consumption, protecting arable land and forest land from converting into other land types, and slowing down the speed of urbanization, and to promote sustainability by controlling rural-to-urban immigration, increasing hazard-free treatment rate of household garbage, and raising energy consumption per unit industrial added value. Although EFA seems more appropriate for estimating LCC for a resource-output or self-sufficient region and ISM is more suitable for a resource-input region, both approaches should be employed when perform LCC assessment in any places around the world. PMID:26121142

Developing a Peak Wind Probability Forecast Tool for Kennedy Space Center and Cape Canaveral Air Force Station

NASA Technical Reports Server (NTRS)

Lambert, WInifred; Roeder, William

2007-01-01

This conference presentation describes the development of a peak wind forecast tool to assist forecasters in determining the probability of violating launch commit criteria (LCC) at Kennedy Space Center (KSC) and Cape Canaveral Air Force Station (CCAFS) in east-central Florida. The peak winds are an important forecast element for both the Space Shuttle and Expendable Launch Vehicle (ELV) programs. The LCC define specific peak wind thresholds for each launch operation that cannot be exceeded in order to ensure the safety of the vehicle. The 45th Weather Squadron (45 WS) has found that peak winds are a challenging parameter to forecast, particularly in the cool season months of October through April. Based on the importance of forecasting peak winds, the 45 WS tasked the Applied Meteorology Unit (AMU) to develop a short-range peak-wind forecast tool to assist in forecasting LCC violations. The tool will include climatologies of the 5-minute mean and peak winds by month, hour, and direction, and probability distributions of the peak winds as a function of the 5-minute mean wind speeds.

Statistical Short-Range Guidance for Peak Wind Forecasts on Kennedy Space Center/Cape Canaveral Air Force Station, Phase III

NASA Technical Reports Server (NTRS)

Crawford, Winifred

2010-01-01

This final report describes the development of a peak wind forecast tool to assist forecasters in determining the probability of violating launch commit criteria (LCC) at Kennedy Space Center (KSC) and Cape Canaveral Air Force Station (CCAFS). The peak winds are an important forecast element for both the Space Shuttle and Expendable Launch Vehicle (ELV) programs. The LCC define specific peak wind thresholds for each launch operation that cannot be exceeded in order to ensure the safety of the vehicle. The 45th Weather Squadron (45 WS) has found that peak winds are a challenging parameter to forecast, particularly in the cool season months of October through April. Based on the importance of forecasting peak winds, the 45 WS tasked the Applied Meteorology Unit (AMU) to develop a short-range peak-wind forecast tool to assist in forecasting LCC violations.The tool includes climatologies of the 5-minute mean and peak winds by month, hour, and direction, and probability distributions of the peak winds as a function of the 5-minute mean wind speeds.

A Peak Wind Probability Forecast Tool for Kennedy Space Center and Cape Canaveral Air Force Station

NASA Technical Reports Server (NTRS)

Crawford, Winifred; Roeder, William

2008-01-01

This conference abstract describes the development of a peak wind forecast tool to assist forecasters in determining the probability of violating launch commit criteria (LCC) at Kennedy Space Center (KSC) and Cape Canaveral Air Force Station (CCAFS) in east-central Florida. The peak winds are an important forecast element for both the Space Shuttle and Expendable Launch Vehicle (ELV) programs. The LCC define specific peak wind thresholds for each launch operation that cannot be exceeded in order to ensure the safety of the vehicle. The 45th Weather Squadron (45 WS) has found that peak winds are a challenging parameter to forecast, particularly in the cool season months of October through April. Based on the importance of forecasting peak winds, the 45 WS tasked the Applied Meteorology Unit (AMU) to develop a short-range peak-wind forecast tool to assist in forecasting LCC violatioas.The tool will include climatologies of the 5-minute mean end peak winds by month, hour, and direction, and probability distributions of the peak winds as a function of the 5-minute mean wind speeds.

Peak Wind Forecasts for the Launch-Critical Wind Towers on Kennedy Space Center/Cape Canaveral Air Force Station, Phase IV

NASA Technical Reports Server (NTRS)

Crawford, Winifred

2011-01-01

This final report describes the development of a peak wind forecast tool to assist forecasters in determining the probability of violating launch commit criteria (LCC) at Kennedy Space Center (KSC) and Cape Canaveral Air Force Station (CCAFS). The peak winds arc an important forecast clement for both the Space Shuttle and Expendable Launch Vehicle (ELV) programs. The LCC define specific peak wind thresholds for each launch operation that cannot be exceeded in order to ensure the safety of the vehicle. The 45th Weather Squadron (45 WS) has found that peak winds are a challenging parameter to forecast, particularly in the cool season months of October through April. Based on the importance of forecasting peak winds, the 45 WS tasked the Applied Meteorology Unit (AMU) to update the statistics in the current peak-wind forecast tool to assist in forecasting LCC violations. The tool includes onshore and offshore flow climatologies of the 5-minute mean and peak winds and probability distributions of the peak winds as a function of the 5-minute mean wind speeds.

Application of Design of Experiments and Surrogate Modeling within the NASA Advanced Concepts Office, Earth-to-Orbit Design Process

NASA Technical Reports Server (NTRS)

Zwack, Matthew R.; Dees, Patrick D.; Holt, James B.

2016-01-01

Decisions made during early conceptual design can have a profound impact on life-cycle cost (LCC). Widely accepted that nearly 80% of LCC is committed. Decisions made during early design must be well informed. Advanced Concepts Office (ACO) at Marshall Space Flight Center aids in decision making for launch vehicles. Provides rapid turnaround pre-phase A and phase A studies. Provides customer with preliminary vehicle sizing information, vehicle feasibility, and expected performance.

ARES I-X Launch Prep

NASA Image and Video Library

2009-10-26

Mission managers, from left, NASA Constellation Program manager Jeff Hanley, Ares I-X Launch Director Ed Mango, Ares I-X mission manager Bob Ess, Ground Operations Manager Philip "Pepper" Phillips, review the latest data in Firing Room One of the Launch Control Center (LCC) at the Kennedy Space Center during the launch countdown of the Ares I-X rocket in Cape Canaveral, Fla., Tuesday, Oct. 27, 2009. The flight test of Ares I-X will provide NASA with an early opportunity to test and prove flight characteristics, hardware, facilities and ground operations associated with the Ares I. Photo Credit: (NASA/Bill Ingalls)

ARES I-X Launch Prep

NASA Image and Video Library

2009-10-26

Mission managers, from left, NASA Ares I-X Assistant Launch Director Pete Nickolenko, Ground Operations Manager Philip "Pepper" Phillips, Ares I-X Launch Director Ed Mango, and Constellation Program manager Jeff Hanley review the latest weather radar from Firing Room One of the Launch Control Center (LCC) at the Kennedy Space Center during the launch countdown of the Ares I-X rocket in Cape Canaveral, Fla., Tuesday, Oct. 27, 2009. The flight test of Ares I-X will provide NASA with an early opportunity to test and prove flight characteristics, hardware, facilities and ground operations associated with the Ares I. Photo Credit: (NASA/Bill Ingalls)

The Other Combustible Products: Prevalence and Correlates of Little Cigar/Cigarillo Use Among Cigarette Smokers.

PubMed

Cohn, Amy; Cobb, Caroline O; Niaura, Raymond S; Richardson, Amanda

2015-12-01

Despite the increasing consumption of little cigars and cigarillos (LCCs), few studies have examined unique predictors and correlates of LCC use among adult cigarette smokers. This study explored differences between cigarette smokers with and without a history of LCC use on harm perceptions, use of other tobacco products (chewing tobacco, snus, e-cigarettes, and dissolvables), cigarette smoking/cessation-related behaviors/cognitions, and mental health and substance use disorder symptoms. A geographically diverse sample of current cigarette smokers were included in analyses (n = 1270). Frequencies of LCC use, awareness, purchase, and harm perceptions were examined and logistic regression models investigated differences between LCC ever and never users on a variety of factors, controlling for demographics. Bivariate analyses showed that LCC users were more likely to be male, younger, have lower income, have tried other tobacco products, perceive LCCs as less harmful than cigarettes, and endorse lifetime substance disorder symptoms. Menthol and other tobacco product use were the only significant correlates of LCC use in logistic regression models. Post-hoc analyses showed that other tobacco product use partially mediated an association between substance use disorder symptoms and LCC use. A third of the sample had tried LCCs, and LCC users were more likely to have experimented with other tobacco products and used menthol. The high degree of co-use of cigarette smoking and LCCs with other tobacco products and the association of LCC use to substance use suggests that these users have unique risk factors and deserve specific targeting in public health campaigns. © The Author 2015. Published by Oxford University Press on behalf of the Society for Research on Nicotine and Tobacco. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

ASCANS Saturn V & LCC Tour

NASA Image and Video Library

2014-03-03

CAPE CANAVERAL, Fla. – NASA astronaut candidates Andrew Morgan, from left, Nicole Mann, Tyler Nick Hague, Josh Cassada, Anne McClain, Christina Hammock and Victor Glover listen to a discussion about firing rooms inside the Launch Control Center at Kennedy Space Center in Florida during a daylong set of briefings and tours of different facilities at NASA's primary launch center. The astronaut class of 2013 was selected by NASA after an extensive year-and-a-half search. The new group will help the agency push the boundaries of exploration and travel to new destinations in the solar system. To learn more about the astronaut class of 2013, visit: http://www.nasa.gov/astronauts/2013astroclass.html Photo credit: NASA/Kim Shiflett

Secretory expression of the non-secretory-type Lentinula edodes laccase by Aspergillus oryzae.

PubMed

Yano, Akira; Kikuchi, Sayaka; Nakagawa, Yuko; Sakamoto, Yuichi; Sato, Toshitsugu

2009-01-01

The shiitake mushroom, Lentinula edodes, has an extracelluar secretory-type laccase, Lcc1, and a fruiting-body-accumulation-type laccase, Lcc4. We previously reported the production of Lcc1 by plant cells, but had difficulty producing Lcc4. Here, we report the production of Lcc1 and Lcc4 by Aspergillus oryzae and the extracellular secretory production of Lcc4 using a modified secretion signal peptide (SP) from Lcc1. Sp-Lcc4 produced by A. oryzae had biochemical activities similar to Lcc4 produced by L. edodes. Lcc1 did not react with beta-(3,4-dihydroxyphenol) alanine (DOPA), but Lcc4 from L. edodes and A. oryzae could oxidize DOPA. K(M) values for the substrates 2,2'-azino-di-(3-ethylbenzthiazolinsulfonate), 2,6-dimethoxyphenol, guaiacol, pyrogallol, and catechol were similar for Lcc4 and Sp-Lcc4. In conclusion, a non-secretory-type fungal laccase is secreted into the culture media with its original enzymatic properties by exploiting modified secretory signal peptide. 2008 Elsevier GmbH.

Differential Regulation by Organic Compounds and Heavy Metals of Multiple Laccase Genes in the Aquatic Hyphomycete Clavariopsis aquatica

PubMed Central

Solé, Magali; Müller, Ines; Pecyna, Marek J.; Fetzer, Ingo; Harms, Hauke

2012-01-01

To advance the understanding of the molecular mechanisms controlling microbial activities involved in carbon cycling and mitigation of environmental pollution in freshwaters, the influence of heavy metals and natural as well as xenobiotic organic compounds on laccase gene expression was quantified using quantitative real-time PCR (qRT-PCR) in an exclusively aquatic fungus (the aquatic hyphomycete Clavariopsis aquatica) for the first time. Five putative laccase genes (lcc1 to lcc5) identified in C. aquatica were differentially expressed in response to the fungal growth stage and potential laccase inducers, with certain genes being upregulated by, e.g., the lignocellulose breakdown product vanillic acid, the endocrine disruptor technical nonylphenol, manganese, and zinc. lcc4 is inducible by vanillic acid and most likely encodes an extracellular laccase already excreted during the trophophase of the organism, suggesting a function during fungal substrate colonization. Surprisingly, unlike many laccases of terrestrial fungi, none of the C. aquatica laccase genes was found to be upregulated by copper. However, copper strongly increases extracellular laccase activity in C. aquatica, possibly due to stabilization of the copper-containing catalytic center of the enzyme. Copper was found to half-saturate laccase activity already at about 1.8 μM, in favor of a fungal adaptation to low copper concentrations of aquatic habitats. PMID:22544244

FPGA for Power Control of MSL Avionics

NASA Technical Reports Server (NTRS)

Wang, Duo; Burke, Gary R.

2011-01-01

A PLGT FPGA (Field Programmable Gate Array) is included in the LCC (Load Control Card), GID (Guidance Interface & Drivers), TMC (Telemetry Multiplexer Card), and PFC (Pyro Firing Card) boards of the Mars Science Laboratory (MSL) spacecraft. (PLGT stands for PFC, LCC, GID, and TMC.) It provides the interface between the backside bus and the power drivers on these boards. The LCC drives power switches to switch power loads, and also relays. The GID drives the thrusters and latch valves, as well as having the star-tracker and Sun-sensor interface. The PFC drives pyros, and the TMC receives digital and analog telemetry. The FPGA is implemented both in Xilinx (Spartan 3- 400) and in Actel (RTSX72SU, ASX72S). The Xilinx Spartan 3 part is used for the breadboard, the Actel ASX part is used for the EM (Engineer Module), and the pin-compatible, radiation-hardened RTSX part is used for final EM and flight. The MSL spacecraft uses a FC (Flight Computer) to control power loads, relays, thrusters, latch valves, Sun-sensor, and star-tracker, and to read telemetry such as temperature. Commands are sent over a 1553 bus to the MREU (Multi-Mission System Architecture Platform Remote Engineering Unit). The MREU resends over a remote serial command bus c-bus to the LCC, GID TMC, and PFC. The MREU also sends out telemetry addresses via a remote serial telemetry address bus to the LCC, GID, TMC, and PFC, and the status is returned over the remote serial telemetry data bus.

The Need for Technology Maturity of Any Advanced Capability to Achieve Better Life Cycle Cost (LCC)

NASA Technical Reports Server (NTRS)

Robinson, John W.; Levack, Daniel J. H.; Rhodes, Russel E.; Chen, Timothy T.

2009-01-01

Programs such as space transportation systems are developed and deployed only rarely, and they have long development schedules and large development and life cycle costs (LCC). They have not historically had their LCC predicted well and have only had an effort to control the DDT&E phase of the programs. One of the factors driving the predictability, and thus control, of the LCC of a program is the maturity of the technologies incorporated in the program. If the technologies incorporated are less mature (as measured by their Technology Readiness Level - TRL), then the LCC not only increases but the degree of increase is difficult to predict. Consequently, new programs avoid incorporating technologies unless they are quite mature, generally TRL greater than or equal to 7 (system prototype demonstrated in a space environment) to allow better predictability of the DDT&E phase costs unless there is no alternative. On the other hand, technology development programs rarely develop technologies beyond TRL 6 (system/subsystem model or prototype demonstrated in a relevant environment). Currently the lack of development funds beyond TRL 6 and the major funding required for full scale development leave little or no funding available to prototype TRL 6 concepts so that hardware would be in the ready mode for safe, reliable and cost effective incorporation. The net effect is that each new program either incorporates little new technology or has longer development schedules and costs, and higher LCC, than planned. This paper presents methods to ensure that advanced technologies are incorporated into future programs while providing a greater accuracy of predicting their LCC. One method is having a dedicated organization to develop X-series vehicles or separate prototypes carried on other vehicles. The question of whether such an organization should be independent of NASA and/or have an independent funding source is discussed. Other methods are also discussed. How to make the choice of which technologies to pursue to the prototype level is also discussed since, to achieve better LCC, first the selection of the appropriate technologies.

Little Cigars and Cigarillos Use Among Young Adult Cigarette Smokers in the United States: Understanding Risk of Concomitant Use Subtypes

PubMed Central

Fryer, Craig S.; Pagano, Ian; Fagan, Pebbles

2016-01-01

Introduction: In 2016, the Food and Drug Administration announced that it would regulate little cigars and cigarillos (LCCs) and expressed concern about the concomitant use of combustible tobacco products. To understand LCC use among socially-disadvantaged cigarette smokers, we assessed (1) the prevalence of concomitant use of subtypes of LCCs: LCC-tobacco, LCC-blunt, and LCC- poly use, which includes use of both LCC-tobacco and LCC-blunt and (2) and its association with sociodemographic factors and substance use behaviors using race/ethnicity and gender stratified models. Methods: In 2015, a web-based survey was administered to a national probability sample of black/African American, Hispanic/Latino, and white cigarette smokers aged 18–44 (n = 1018). Weighted estimates were used to assess current LCC-tobacco, LCC-blunt, and LCC-poly use. Multinomial regression models assessed sociodemographic, other tobacco and substance use correlates associated with LCC user subtypes. Results: Of cigarette smokers, 63% did not smoke LCCs; 15.1% were LCC-tobacco users; 11.1% were LCC-blunt users; and 10.5% were LCC-poly users. Black/African American and Hispanic/Latino cigarette smokers had higher odds of LCC-tobacco, LCC-blunt, and LCC-poly use compared to white cigarette smokers. Blacks/African Americans who initiated cigarette smoking before age 18 and smoked other tobacco products had greater odds of LCC-tobacco use than whites. Male cigarette smokers who smoked other tobacco products and females who had early onset of cigarette use also had greater odds of LCC-tobacco use. Conclusions: Over 30% of cigarette smokers concomitantly used LCCs, which may prolong smoking. Accurate estimates of diverse LCC use behaviors may increase our understanding of the potential harms of concomitant use. Implications: Aggregate measures of LCC smoking do not distinguish subtypes of use among socially-disadvantaged cigarette smokers (ie, young adults, blacks/African Americans, Hispanics/Latinos), who may engage in these unique smoking behaviors. We document the prevalence of young adult cigarette smokers who dual use LCC-tobacco and LCC-blunts and are poly users of LCC-tobacco + LCC-blunts, and identify sociodemographic groups at risk for use. The Food and Drug Administration is concerned about concomitant behavior, which may increase chronic disease risk and addiction. Accurate estimates of LCC smoking behaviors may increase our understanding of the harms of concomitant use; which can inform prevention programs that specifically target LCC subtypes. PMID:27613889

KSC-2009-5248

NASA Image and Video Library

2009-09-25

CAPE CANAVERAL, Fla. – This ribbon cutting officially turns over NASA Kennedy Space Center's Launch Control Center Firing Room 1 from the Space Shuttle Program to the Constellation Program. Participating are (from left) Pepper Phillips, director of the Constellation Project Office at Kennedy; Bob Cabana, Kennedy's director; Robert Crippen, former astronaut; Jeff Hanley, manager of the Constellation Program at NASA's Johnson Space Center; and Nancy Bray, deputy director of Center Operations at Kennedy. The room has undergone demolition and construction and been outfitted with consoles for the upcoming Ares I-X rocket flight test targeted for launch on Oct. 27. As the center of launch operations at Kennedy since the Apollo Program, the Launch Control Center, or LCC, has played a central role in NASA's human spaceflight programs. Firing Room 1 was the first operational firing room constructed. From this room, controllers launched the first Saturn V, the first crewed flight of Saturn V, the first crewed mission to the moon and the first space shuttle. Firing Room 1 will continue this tradition of firsts when controllers launch the Constellation Program's first flight test. Also, this firing room will be the center of operations for the upcoming Ares I and Orion operations. Photo credit: NASA/Kim Shiflett

Mitigation of commutation failures in LCC-HVDC systems based on superconducting fault current limiters

NASA Astrophysics Data System (ADS)

Lee, Jong-Geon; Khan, Umer Amir; Lee, Ho-Yun; Lim, Sung-Woo; Lee, Bang-Wook

2016-11-01

Commutation failure in line commutated converter based HVDC systems cause severe damages on the entire power grid system. For LCC-HVDC, thyristor valves are turned on by a firing signal but turn off control is governed by the external applied AC voltage from surrounding network. When the fault occurs in AC system, turn-off control of thyristor valves is unavailable due to the voltage collapse of point of common coupling (PCC), which causes the commutation failure in LCC-HVDC link. Due to the commutation failure, the power transfer interruption, dc voltage drop and severe voltage fluctuation in the AC system could be occurred. In a severe situation, it might cause the protection system to block the valves. In this paper, as a solution to prevent the voltage collapse on PCC and to limit the fault current, the application study of resistive superconducting fault current limiter (SFCL) on LCC-HVDC grid system was performed with mathematical and simulation analyses. The simulation model was designed by Matlab/Simulink considering Haenam-Jeju HVDC power grid in Korea which includes conventional AC system and onshore wind farm and resistive SFCL model. From the result, it was observed that the application of SFCL on LCC-HVDC system is an effective solution to mitigate the commutation failure. And then the process to determine optimum quench resistance of SFCL which enables the recovery of commutation failure was deeply investigated.

KSC-2011-7994

NASA Image and Video Library

2011-11-26

CAPE CANAVERAL, Fla. – At NASA Kennedy Space Center in Florida, Will.i.am, entertainer and member of The Black Eyed Peas, hears about the space shuttle mission plaques lining the lobby of the Launch Control Center (LCC) from NASA's Charlie Blackwell Thompson. Also viewing the plaques in the background are NASA Deputy Administrator Lori Garver and former astronaut Leland Melvin, NASA associate administrator for Education. The visit to the LCC followed their participation in a NASA Tweetup. The Tweetup is part of prelaunch activities for the agency’s Mars Science Laboratory (MSL) launch and provides the opportunity for tweeters will share their experiences with followers through the social networking site Twitter. The MSL mission will pioneer precision landing technology and a sky-crane touchdown to place a car-sized rover, Curiosity, near the foot of a mountain inside Gale Crater on Aug. 6, 2012. During a nearly two-year prime mission after landing, the rover will investigate whether the region has ever offered conditions favorable for microbial life, including the chemical ingredients for life. Liftoff of MSL aboard a United Launch Alliance Atlas V rocket from Space Launch Complex-41 on Cape Canaveral Air Force Station was at 10:02 a.m. EST on Nov. 26. For more information, visit http://www.nasa.gov/msl. Photo credit: NASA/Jim Grossmann

A Life-Cycle Cost Estimating Methodology for NASA-Developed Air Traffic Control Decision Support Tools

NASA Technical Reports Server (NTRS)

Wang, Jianzhong Jay; Datta, Koushik; Landis, Michael R. (Technical Monitor)

2002-01-01

This paper describes the development of a life-cycle cost (LCC) estimating methodology for air traffic control Decision Support Tools (DSTs) under development by the National Aeronautics and Space Administration (NASA), using a combination of parametric, analogy, and expert opinion methods. There is no one standard methodology and technique that is used by NASA or by the Federal Aviation Administration (FAA) for LCC estimation of prospective Decision Support Tools. Some of the frequently used methodologies include bottom-up, analogy, top-down, parametric, expert judgement, and Parkinson's Law. The developed LCC estimating methodology can be visualized as a three-dimensional matrix where the three axes represent coverage, estimation, and timing. This paper focuses on the three characteristics of this methodology that correspond to the three axes.

Space Transportation Systems Life Cycle Cost Assessment and Control

NASA Technical Reports Server (NTRS)

Robinson, John W.; Rhodes, Russell E.; Zapata, Edgar; Levack, Daniel J. H.; Donahue, Benjaamin B.; Knuth, William

2008-01-01

Civil and military applications of space transportation have been pursued for just over 50 years and there has been, and still is, a need for safe, dependable, affordable, and sustainable space transportation systems. Fully expendable and partially reusable space transportation systems have been developed and put in operation that have not adequately achieved this need. Access to space is technically achievable, but presently very expensive and will remain so until there is a breakthrough in the way we do business. Since 1991 the national Space Propulsion Synergy Team (SPST) has reviewed and assessed the lessons learned from the major U.S. space programs of the past decades focusing on what has been learned from the assessment and control of Life Cycle Cost (LCC) from these systems. This paper presents the results of a selected number of studies and analyses that have been conducted by the SPST addressing the need, as well as the solutions, for improvement in LCC. The major emphasis of the SPST processes is on developing the space transportation system requirements first (up front). These requirements must include both the usual system flight performance requirements and also the system functional requirements, including the infrastructure on Earth's surface, in-space and on the Moon and Mars surfaces to determine LCC. This paper describes the development of specific innovative engineering and management approaches and processes. This includes a focus on flight hardware maturity for reliability, ground operations approaches, and business processes between contractor and government organizations. A major change in program/project cost control is being proposed by the SPST to achieve a sustainable space transportation system LCC - controlling cost as a program metric in addition to the existing practice of controlling performance and weight. Without a firm requirement and methodically structured cost control, it is unlikely that an affordable and sustainable space transportation system LCC will ever be achieved. '

Comparative molecular analysis of early and late cancer cachexia-induced muscle wasting in mouse models.

PubMed

Sun, Rulin; Zhang, Santao; Lu, Xing; Hu, Wenjun; Lou, Ning; Zhao, Yan; Zhou, Jia; Zhang, Xiaoping; Yang, Hongmei

2016-12-01

Cancer-induced muscle wasting, which commonly occurs in cancer cachexia, is characterized by impaired quality of life and poor patient survival. To identify an appropriate treatment, research on the mechanism underlying muscle wasting is essential. Thus far, studies on muscle wasting using cancer cachectic models have generally focused on early cancer cachexia (ECC), before severe body weight loss occurs. In the present study, we established models of ECC and late cancer cachexia (LCC) and compared different stages of cancer cachexia using two cancer cachectic mouse models induced by colon-26 (C26) adenocarcinoma or Lewis lung carcinoma (LLC). In each model, tumor-bearing (TB) and control (CN) mice were injected with cancer cells and PBS, respectively. The TB and CN mice, which were euthanized on the 24th day or the 36th day after injection, were defined as the ECC and ECC-CN mice or the LCC and LCC-CN mice. In addition, the tissues were harvested and analyzed. We found that both the ECC and LCC mice developed cancer cachexia. The amounts of muscle loss differed between the ECC and LCC mice. Moreover, the expression of some molecules was altered in the muscles from the LCC mice but not in those from the ECC mice compared with their CN mice. In conclusion, the molecules with altered expression in the muscles from the ECC and LCC mice were not exactly the same. These findings may provide some clues for therapy which could prevent the muscle wasting in cancer cachexia from progression to the late stage.

A Hybrid LCC-VSC HVDC Transmission System Supplying a Passive Load

NASA Astrophysics Data System (ADS)

Kotb, Omar

High Voltage Direct Current (HVDC) transmission systems continue to be an excellent asset in modern power systems, mainly for their ability to overcome the problems of AC transmission, such as the interconnection of asynchronous grids, stability of long transmission lines, and use of long cables for power transmission. In the past 20 years, Voltage Source Converter (VSC)-HVDC transmission systems were developed and installed in many projects, thereby adding more operational benefits to DC transmission option, such as high controllability, ability to supply weak networks, and reduced converter reactive power demand. Nevertheless, VSC-HVDC transmission suffers from the disadvantages of high losses and cost. In this research, a hybrid HVDC employing a Line Commutated Converter (LCC) as rectifier and a VSC as inverter is used to supply a passive network through a DC cable. The hybrid system is best suited for unidirectional power transmission scenarios, such as power transmission to islands and remote load centers, where the construction of new transmission lines is prohibitively expensive. Control modes for the rectifier and inverter are selected and implemented using Proportional Integral (PI) controllers. Special control schemes are developed for abnormal operating conditions such as starting at light load and recovering from AC network faults. The system performance under steady state and transient conditions is investigated by EMTP-RV simulations. The results show the feasibility of the hybrid system.

Small-Signal Dynamic Analysis of LCC-HVDC with STATCOM at the Inverter Busbar

NASA Astrophysics Data System (ADS)

Liu, Dong; Jiang, Wen; Guo, Chunyi; Rehman, Atiq Ur; Zhao, Chengyong

2018-01-01

This paper develops a linearized small-signal dynamic model of a Line-Commutated-Converter based HVDC (LCC-HVDC) system with STATCOM at the inverter busbar, and validates its accuracy by comparing time-domain responses from small-signal model and PSCAD-based simulation results. Considering the potential impact of Phase-Locked-Loop (PLL) parameters on the study system and the close connection of STATCOM and LCC inverter station at AC busbar, this paper investigates the impact of PLL gains and AC voltage control parameters of STATCOM on the system small-signal stability. The studies show that (i) the PLL gain has highly impact on the study system and smaller PLL gains are preferable; (ii) larger values of both the proportional gain and the integral gain of AC voltage controller of STATCOM could result in oscillation/instability of the system.

Planning and Estimation of Operations Support Requirements

NASA Technical Reports Server (NTRS)

Newhouse, Marilyn E.; Barley, Bryan; Bacskay, Allen; Clardy, Dennon

2010-01-01

Life Cycle Cost (LCC) estimates during the proposal and early design phases, as well as project replans during the development phase, are heavily focused on hardware development schedules and costs. Operations (phase E) costs are typically small compared to the spacecraft development and test costs. This, combined with the long lead time for realizing operations costs, can lead to de-emphasizing estimation of operations support requirements during proposal, early design, and replan cost exercises. The Discovery and New Frontiers (D&NF) programs comprise small, cost-capped missions supporting scientific exploration of the solar system. Any LCC growth can directly impact the programs' ability to fund new missions, and even moderate yearly underestimates of the operations costs can present significant LCC impacts for deep space missions with long operational durations. The National Aeronautics and Space Administration (NASA) D&NF Program Office at Marshall Space Flight Center (MSFC) recently studied cost overruns and schedule delays for 5 missions. The goal was to identify the underlying causes for the overruns and delays, and to develop practical mitigations to assist the D&NF projects in identifying potential risks and controlling the associated impacts to proposed mission costs and schedules. The study found that 4 out of the 5 missions studied had significant overruns at or after launch due to underestimation of the complexity and supporting requirements for operations activities; the fifth mission had not launched at the time of the mission. The drivers behind these overruns include overly optimistic assumptions regarding the savings resulting from the use of heritage technology, late development of operations requirements, inadequate planning for sustaining engineering and the special requirements of long duration missions (e.g., knowledge retention and hardware/software refresh), and delayed completion of ground system development work. This paper updates the D&NF LCC study, looking at the operations (phase E) cost drivers in more detail and extending the study to include 2 additional missions and identifies areas for increased emphasis by project management in order to improve the fidelity of operations estimates.

ASCANS Saturn V & LCC Tour

NASA Image and Video Library

2014-03-03

CAPE CANAVERAL, Fla. – NASA astronaut candidates Andrew Morgan, from left, Nicole Mann, Tyler Nick Hague, Josh Cassada, Anne McClain, Christina Hammock and Victor Glover listen as Steve Cox or Flight Systems and Operations Integration in Kennedy Ground Systems Development and Operations, far right, briefed on firing rooms inside the Launch Control Center at Kennedy Space Center in Florida during a daylong set of briefings and tours of different facilities at NASA's primary launch center. The astronaut class of 2013 was selected by NASA after an extensive year-and-a-half search. The new group will help the agency push the boundaries of exploration and travel to new destinations in the solar system. To learn more about the astronaut class of 2013, visit: http://www.nasa.gov/astronauts/2013astroclass.html Photo credit: NASA/Kim Shiflett

Basal activity of voltage-gated Ca(2+) channels controls the IP3-mediated contraction by α(1)-adrenoceptor stimulation of mouse aorta segments.

PubMed

Leloup, Arthur J; Van Hove, Cor E; De Meyer, Guido R Y; Schrijvers, Dorien M; Fransen, Paul

2015-08-05

α1-Adrenoceptor stimulation of mouse aorta causes intracellular Ca(2+) release from sarcoplasmic reticulum Ca(2+) stores via stimulation of inositoltriphosphate (IP3) receptors. It is hypothesized that this Ca(2+) release from the contractile and IP3-sensitive Ca(2+) store is under the continuous dynamic control of time-independent basal Ca(2+) influx via L-type voltage-gated Ca(2+) channels (LCC) residing in their window voltage range. Mouse aortic segments were α1-adrenoceptor stimulated with phenylephrine in the absence of external Ca(2+) (0Ca) to measure phasic isometric contractions. They gradually decreased with time in 0Ca, were inhibited with 2-aminoethoxydiphenyl borate, and declined with previous membrane potential hyperpolarization (levcromakalim) or with previous inhibition of LCC (diltiazem). Former basal stimulation of LCC with depolarization (15 mM K(+)) or with BAY K8644 increased the subsequent phasic contractions by phenylephrine in 0Ca. Although exogenous NO (diethylamine NONOate) reduced the phasic contractions by phenylephrine, stimulation of endothelial cells with acetylcholine in 0Ca failed to attenuate these phasic contractions. Finally, inhibition of the basal release of NO with N(Ω)-nitro-L-arginine methyl ester also attenuated the phasic contractions by phenylephrine. Results indicated that α1-adrenoceptor stimulation with phenylephrine causes phasic contractions, which are controlled by basal LCC and endothelial NO synthase activity. Endothelial NO release by acetylcholine was absent in 0Ca. Given the growing interest in the active regulation of arterial compliance, the dependence of contractile SR Ca(2+) store-refilling in basal conditions on the activity of LCC and basal eNOS may contribute to a more thorough understanding of physiological mechanisms leading to arterial stiffness. Copyright © 2015. Published by Elsevier B.V.

The effect of life-cycle cost disclosure on consumer behavior

NASA Astrophysics Data System (ADS)

Deutsch, Matthias

For more than 20 years, analysts have reported on the so-called "energy paradox" or the "energy efficiency gap", referring to the fact that economic agents could in principle lower their total cost at current prices by using more energy-efficient technology but, nevertheless, often decide not to do so. Theory suggests that providing information in a simplified way could potentially reduce this "efficiency gap". Such simplification may be achieved by providing the estimated monetary operating cost and life-cycle cost (LCC) of a given appliance---which has been a recurring theme within the energy policy and efficiency labeling community. Yet, little is known so far about the causal effects of LCC disclosure on consumer action because of the gap between the acquisition of efficiency information and consumer purchasing behavior in the real marketplace. This dissertation bridges the gap by experimentally integrating LCC disclosure into two major German commercial websites---a price comparison engine for cooling appliances, and an online shop for washing machines. Internet users arriving on these websites were randomly assigned to two experimental groups, and the groups were exposed to different visual stimuli. The control group received regular product price information, whereas the treatment group was, in addition, offered information about operating cost and total LCC. Click-stream data of consumers' shopping behavior was evaluated with multiple regression analysis by controlling for several product characteristics. This dissertation finds that LCC disclosure reduces the mean energy use of chosen cooling appliances by 2.5% (p<0.01), and the energy use of chosen washing machines by 0.8% (p<0.001). For the latter, it also reduces the mean water use by 0.7% (p<0.05). These effects suggest a potential role for public policy in promoting LCC disclosure. While I do not attempt to estimate the costs of such a policy, a simple quantification shows that the benefits amount to 100 to 200 thousand Euros per year for Germany, given current predictions regarding the price of tradable permits for CO2, and not counting other potential benefits. Future research should strive for increasing external validity, using better instruments, and evaluating the effectiveness of different information formats for LCC disclosure.

Linking Statistically- and Physically-Based Models for Improved Streamflow Simulation in Gaged and Ungaged Areas

NASA Astrophysics Data System (ADS)

Lafontaine, J.; Hay, L.; Archfield, S. A.; Farmer, W. H.; Kiang, J. E.

2014-12-01

The U.S. Geological Survey (USGS) has developed a National Hydrologic Model (NHM) to support coordinated, comprehensive and consistent hydrologic model development, and facilitate the application of hydrologic simulations within the continental US. The portion of the NHM located within the Gulf Coastal Plains and Ozarks Landscape Conservation Cooperative (GCPO LCC) is being used to test the feasibility of improving streamflow simulations in gaged and ungaged watersheds by linking statistically- and physically-based hydrologic models. The GCPO LCC covers part or all of 12 states and 5 sub-geographies, totaling approximately 726,000 km2, and is centered on the lower Mississippi Alluvial Valley. A total of 346 USGS streamgages in the GCPO LCC region were selected to evaluate the performance of this new calibration methodology for the period 1980 to 2013. Initially, the physically-based models are calibrated to measured streamflow data to provide a baseline for comparison. An enhanced calibration procedure then is used to calibrate the physically-based models in the gaged and ungaged areas of the GCPO LCC using statistically-based estimates of streamflow. For this application, the calibration procedure is adjusted to address the limitations of the statistically generated time series to reproduce measured streamflow in gaged basins, primarily by incorporating error and bias estimates. As part of this effort, estimates of uncertainty in the model simulations are also computed for the gaged and ungaged watersheds.

KSC-04PD-0244

NASA Technical Reports Server (NTRS)

2004-01-01

KENNEDY SPACE CENTER, FLA. In the Launch Control Center, Robert Holl (left), Landing Recovery directo, and Donald Hammel, from the Shuttle Project Office, are in contact with the leaders of the Mode VII emergency landing simulation at Kennedy Space Center. The simulation is being managed and directed from the LCC. The purpose of the Mode VII is to exercise emergency preparedness personnel, equipment and facilities in rescuing astronauts from a downed orbiter and providing immediate medical attention. This simulation presents an orbiter that has crashed short of the Shuttle Landing Facility in a wooded area 2-1/2 miles south of Runway 33. Emergency crews are responding to the volunteer astronauts who are simulating various injuries inside the crew compartment mock-up. Rescuers must remove the crew, provide triage and transport to hospitals those who need further treatment. Local hospitals are participating in the exercise.

Production, purification and biochemical characterization of two laccase isoforms produced by Trametes versicolor grown on oak sawdust.

PubMed

Martínez-Morales, Fernando; Bertrand, Brandt; Pasión Nava, Angélica A; Tinoco, Raunel; Acosta-Urdapilleta, Lourdes; Trejo-Hernández, María R

2015-02-01

Two laccase isoforms (lcc1 and lcc2) produced by Trametes versicolor, grown on oak sawdust under solid-state fermentation conditions, were purified and characterized. The two isoforms showed significant biochemical differences. Lcc1 and lcc2 had MWs of 60 and 100 kDa, respectively. Both isoforms had maximal activity at pH 3 with ABTS and 2,6-dimethyloxyphenol (DMP). Lcc1 was the most attractive isoform due to its greater affinity towards all the laccase substrates used. Lcc1 had Km values of 12, 10, 15 and 17 mM towards ABTS, DMP, guaiacol and syringaldazine, respectively. Lcc2 had equivalent values of 45, 47, 15 and 39 mM. The biochemical properties of lcc1 substantiate the potential of this enzyme for application in the treatment of contaminated water with low pH values and high phenolic content.

Comparison of two laccases from Trametes versicolor for application in the decolorization of dyes.

PubMed

Li, Qi; Ge, Lin; Cai, Junli; Pei, Jianjun; Xie, Jingcong; Zhao, Linguo

2014-04-01

It has been previously demonstrated that laccases exhibit great potential for use in several industrial and environmental applications. In this paper, two laccase isoenzyme genes, lccB and lccC, were cloned and expressed in Pichia pastoris GS115. The sequence analysis indicated that the lccB and lccC genes consisted of 1,563 and 1,584 bp, and their open reading frames encoded 520 and 527 amino acids, respectively. They had 72.7% degree of identity in nucleotides and 86.7% in amino acids. The expression levels of LccB and LccC were up to 32,479 and 34,231 U/l, respectively. The recombinant laccases were purified by ultrafiltration and (NH4)2SO4 precipitation, showing a single band on SDS-PAGE, which had a molecular mass of 58 kDa. The optimal pH and temperature for LccB were 2.0 and 55°C with 2,2'-azino-bis-[3-ethylbenzthiazolinesulfonic acid (ABTS) as a substrate, whereas LccC exhibited optimal pH and temperature at 3.0 and 60°C. The apparent kinetic parameters of LccB were 0.43 mM for ABTS with a Vmax value of 51.28 U/mg, and the Km and Vmax values for LccC were 0.29 mM and 62.89 U/mg. The recombinant laccases were able to decolorize five types of dyes. Acid Violet 43 (100 g/ml) was completely decolorized by LccB or LccC (2 U/ml), and the decolorization of Reactive Blue KN-R (100 g/ml) was 91.6% by LccC (2 U/ml). Thus, the study characterizes useful laccase isoenzymes from T. versicolor that have the capability of being incorporated into the treatment of similar azo and anthraquinone dyes from dyeing industries.

Software and Human-Machine Interface Development for Environmental Controls Subsystem Support

NASA Technical Reports Server (NTRS)

Dobson, Matthew

2018-01-01

The Space Launch System (SLS) is the next premier launch vehicle for NASA. It is the next stage of manned space exploration from American soil, and will be the platform in which we push further beyond Earth orbit. In preparation of the SLS maiden voyage on Exploration Mission 1 (EM-1), the existing ground support architecture at Kennedy Space Center required significant overhaul and updating. A comprehensive upgrade of controls systems was necessary, including programmable logic controller software, as well as Launch Control Center (LCC) firing room and local launch pad displays for technician use. Environmental control acts as an integral component in these systems, being the foremost system for conditioning the pad and extremely sensitive launch vehicle until T-0. The Environmental Controls Subsystem (ECS) required testing and modification to meet the requirements of the designed system, as well as the human factors requirements of NASA software for Validation and Verification (V&V). This term saw significant strides in the progress and functionality of the human-machine interfaces used at the launch pad, and improved integration with the controller code.

Balanced Electrostatic and Structural Forces Guide the Large Conformational Change Associated with Maturation of T = 4 Virus

PubMed Central

Matsui, Tsutomu; Tsuruta, Hiro; Johnson, John E.

2010-01-01

Nudaurelia capensis omega virus has a well-characterized T = 4 capsid that undergoes a pH-dependent large conformational changes (LCC) and associated auto-catalytic cleavage of the subunit. We examined previously the particle size at different pH values and showed that maturation occurred at pH 5.5. We now characterized the LCC with time-resolved small-angle x-ray scattering and showed that there were three kinetic stages initiated with an incremental drop in pH: 1), a rapid (<10 ms) collapse to an incrementally smaller particle; 2), a continuous size reduction over the next 5 s; and 3), a smaller final transition occurring in 2–3 min. Equilibrium measurements similar to those reported previously, but now more precise, showed that the particle dimension between pH 5.5 and 5 requires the autocatalytic cleavage to achieve its final compact size. A balance of electrostatic and structural forces shapes the energy landscape of the LCC with the latter requiring annealing of portions of the subunit. Equilibrium experiments showed that many intermediate states could be populated with a homogeneous ensemble of particles by carefully controlling the pH. A titration curve for the LCC was generated that showed that the virtual pKa (i.e., the composite of all titratable residues that contribute to the LCC) is 5.8. PMID:20371334

KSC-04PD-0245

NASA Technical Reports Server (NTRS)

2004-01-01

KENNEDY SPACE CENTER, FLA. In the Launch Control Center, officials monitor the Mode VII emergency landing simulation being conducted at Kennedy Space Center and managed and directed from the LCC. From left are Dr. Luis Moreno and Dr. David Reed, with Bionetics Life Sciences, and Dr. Philip Scarpa, with the KSC Safety, Occupational Health and Environment Division. The purpose of the Mode VII is to exercise emergency preparedness personnel, equipment and facilities in rescuing astronauts from a downed orbiter and providing immediate medical attention. This simulation presents an orbiter that has crashed short of the Shuttle Landing Facility in a wooded area 2-1/2 miles south of Runway 33. Emergency crews are responding to the volunteer astronauts who are simulating various injuries inside the crew compartment mock-up. Rescuers must remove the crew, provide triage and transport to hospitals those who need further treatment. Local hospitals are participating in the exercise.

Nuclear respiratory factor-1 and bioenergetics in tamoxifen-resistant breast cancer cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Radde, Brandie N.; Ivanova, Margarita M.; Mai, Huy Xuan

Acquired tamoxifen (TAM) resistance is a significant clinical problem in treating patients with estrogen receptor α (ERα)+ breast cancer. We reported that ERα increases nuclear respiratory factor-1 (NRF-1), which regulates nuclear-encoded mitochondrial gene transcription, in MCF-7 breast cancer cells and NRF-1 knockdown stimulates apoptosis. Whether NRF-1 and target gene expression is altered in endocrine resistant breast cancer cells is unknown. We measured NRF-1and metabolic features in a cell model of progressive TAM-resistance. NRF-1 and its target mitochondrial transcription factor A (TFAM) were higher in TAM-resistant LCC2 and LCC9 cells than TAM-sensitive MCF-7 cells. Using extracellular flux assays we observed thatmore » LCC1, LCC2, and LCC9 cells showed similar oxygen consumption rate (OCR), but lower mitochondrial reserve capacity which was correlated with lower Succinate Dehydrogenase Complex, Subunit B in LCC1 and LCC2 cells. Complex III activity was lower in LCC9 than MCF-7 cells. LCC1, LCC2, and LCC9 cells had higher basal extracellular acidification (ECAR), indicating higher aerobic glycolysis, relative to MCF-7 cells. Mitochondrial bioenergetic responses to estradiol and 4-hydroxytamoxifen were reduced in the endocrine-resistant cells compared to MCF-7 cells. These results suggest the acquisition of altered metabolic phenotypes in response to long term antiestrogen treatment may increase vulnerability to metabolic stress. - Highlights: • NRF-1 and TFAM expression are higher in endocrine-resistant breast cancer cells. • Oxygen consumption rate is similar in endocrine-sensitive and resistant cells. • Mitochondrial reserve capacity is lower in endocrine-resistant cells. • Endocrine-resistant breast cancer cells have increased glycolysis. • Bioenergetic responses to E2 and tamoxifen are lower in endocrine-resistant cells.« less

Risk Perceptions of Little Cigar and Cigarillo Smoking Among Adult Current Cigarette Smokers.

PubMed

Sterling, Kymberle L; Majeed, Ban A; Nyman, Amy; Eriksen, Michael

2017-11-01

Few studies have examined the perceptions of risk of little cigar and cigarillo (LCC) smoking among cigarette smokers, which is important for expanding regulatory policies and developing prevention programs. We examined current cigarette smokers' perceived harm of LCC smoking, and determined whether these perceptions were associated with susceptibility and intention to continue smoking LCCs. Data were from the 2014 Tobacco Products and Risk Perceptions Survey of a probability sample of 5717 US adults. Data were analyzed for a subsample of 1191 current cigarette smokers who were stratified into three groups: (1) dual current cigarette smokers who had ever used LCCs, (2) current smokers susceptible to LCC smoking, and (3) current smokers who were not susceptible to LCC smoking. Overall, 47.2% of participants were dual smokers, 12.7% were susceptible to LCC smoking, and 40.1% were not susceptible. Perceptions of risk of LCCs varied across the groups. Dual smokers were more likely to perceive that daily LCC smoking is "very risky" (OR = 1.64, 95% CI = 1.08, 2.41) while occasional LCC smoking is only "somewhat risky" (OR = 1.71, 95% CI = 1.02, 2.87). Of the dual smokers, 20.7% intended to continue smoking LCCs in the future. Perceptions of addiction and risk of daily LCC smoking significantly predicted intention to continue LCC smoking. Addiction perceptions also significantly predicted susceptibility to initiate LCC smoking. Perceptions about harms from and addiction to LCCs could predict future LCC smoking. Health communication campaigns need to address the harms of LCCs. Our data suggest that perceptions of risk about the addictiveness of LCCs and frequency of use are important determinants of the LCC smoking susceptibility among some cigarette smokers and intended continued use among cigarette smokers with a history of LCC use. Health communication campaigns should address misperceptions related to LCCs. © The Author 2016. Published by Oxford University Press on behalf of the Society for Research on Nicotine and Tobacco. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Design and Development of Functionally Operative and Visually Appealing Remote Firing Room Displays

NASA Technical Reports Server (NTRS)

Quaranto, Kristy

2014-01-01

This internship provided an opportunity for an intern to work with NASA's Ground Support Equipment (GSE) for the Spaceport Command and Control System (SCCS) at Kennedy Space Center as a remote display developer, under NASA mentor Kurt Leucht. The main focus was on creating remote displays for the hypergolic and high pressure helium subsystem team to help control the filling of the respective tanks. As a remote display developer for the GSE hypergolic and high pressure helium subsystem team the intern was responsible for creating and testing graphical remote displays to be used in the Launch Control Center (LCC) on the Firing Room's computer monitors. To become more familiar with the subsystem, the individual attended multiple project meetings and acquired their specific requirements regarding what needed to be included in the remote displays. After receiving the requirements, the next step was to create a display that had both visual appeal and logical order using the Display Editor, on the Virtual Machine (VM). In doing so, all Compact Unique Identifiers (CUI), which are associated with specific components within the subsystem, will need to be included in each respective display for the system to run properly. Then, once the display was created it needed to be tested to ensure that the display runs as intended by using the Test Driver, also found on the VM. This Test Driver is a specific application that checks to make sure all the CUIs in the display are running properly and returning the correct form of information. After creating and locally testing the display it will need to go through further testing and evaluation before deemed suitable for actual use. By the end of the semester long experience at NASA's Kennedy Space Center, the individual should have gained great knowledge and experience in various areas of display development and testing. They were able to demonstrate this new knowledge obtained by creating multiple successful remote displays that will one day be used by the hypergolic and high pressure helium subsystem team in one of the LCC's firing rooms to fill the new Orion spacecraft.

Inhibition of Aerobic Glycolysis Represses Akt/mTOR/HIF-1α Axis and Restores Tamoxifen Sensitivity in Antiestrogen-Resistant Breast Cancer Cells

PubMed Central

Woo, Yu Mi; Shin, Yubin; Lee, Eun Ji; Lee, Sunyoung; Jeong, Seung Hun; Kong, Hyun Kyung; Park, Eun Young; Kim, Hyoung Kyu; Han, Jin; Chang, Minsun; Park, Jong-Hoon

2015-01-01

Tamoxifen resistance is often observed in the majority of estrogen receptor–positive breast cancers and it remains as a serious clinical problem in breast cancer management. Increased aerobic glycolysis has been proposed as one of the mechanisms for acquired resistance to chemotherapeutic agents in breast cancer cells such as adriamycin. Herein, we report that the glycolysis rates in LCC2 and LCC9—tamoxifen-resistant human breast cancer cell lines derived from MCF7— are higher than those in MCF7S, which is the parent MCF7 subline. Inhibition of key glycolytic enzyme such as hexokinase-2 resulted in cell growth retardation at higher degree in LCC2 and LCC9 than that in MCF7S. This implies that increased aerobic glycolysis even under O2-rich conditions, a phenomenon known as the Warburg effect, is closely associated with tamoxifen resistance. We found that HIF-1α is activated via an Akt/mTOR signaling pathway in LCC2 and LCC9 cells without hypoxic condition. Importantly, specific inhibition of hexokinase-2 suppressed the activity of Akt/mTOR/HIF-1α axis in LCC2 and LCC9 cells. In addition, the phosphorylated AMPK which is a negative regulator of mTOR was decreased in LCC2 and LCC9 cells compared to MCF7S. Interestingly, either the inhibition of mTOR activity or increase in AMPK activity induced a reduction in lactate accumulation and cell survival in the LCC2 and LCC9 cells. Taken together, our data provide evidence that development of tamoxifen resistance may be driven by HIF-1α hyperactivation via modulation of Akt/mTOR and/or AMPK signaling pathways. Therefore, we suggest that the HIF-1α hyperactivation is a critical marker of increased aerobic glycolysis in accordance with tamoxifen resistance and thus restoration of aerobic glycolysis may be novel therapeutic target for treatment of tamoxifen-resistant breast cancer. PMID:26158266

Observations of Local Positive Low Cloud Feedback Patterns and Their Role in Internal Variability and Climate Sensitivity

NASA Astrophysics Data System (ADS)

Yuan, Tianle; Oreopoulos, Lazaros; Platnick, Steven E.; Meyer, Kerry

2018-05-01

Modeling studies have shown that cloud feedbacks are sensitive to the spatial pattern of sea surface temperature (SST) anomalies, while cloud feedbacks themselves strongly influence the magnitude of SST anomalies. Observational counterparts to such patterned interactions are still needed. Here we show that distinct large-scale patterns of SST and low-cloud cover (LCC) emerge naturally from objective analyses of observations and demonstrate their close coupling in a positive local SST-LCC feedback loop that may be important for both internal variability and climate change. The two patterns that explain the maximum amount of covariance between SST and LCC correspond to the Interdecadal Pacific Oscillation and the Atlantic Multidecadal Oscillation, leading modes of multidecadal internal variability. Spatial patterns and time series of SST and LCC anomalies associated with both modes point to a strong positive local SST-LCC feedback. In many current climate models, our analyses suggest that SST-LCC feedback strength is too weak compared to observations. Modeled local SST-LCC feedback strength affects simulated internal variability so that stronger feedback produces more intense and more realistic patterns of internal variability. To the extent that the physics of the local positive SST-LCC feedback inferred from observed climate variability applies to future greenhouse warming, we anticipate significant amount of delayed warming because of SST-LCC feedback when anthropogenic SST warming eventually overwhelm the effects of internal variability that may mute anthropogenic warming over parts of the ocean. We postulate that many climate models may be underestimating both future warming and the magnitude of modeled internal variability because of their weak SST-LCC feedback.

Molecular characterization of thyroid hormone-inhibited atrial L-type calcium channel expression: implication for atrial fibrillation in hyperthyroidism.

PubMed

Chen, Wei-Jan; Yeh, Yung-Hsin; Lin, Kwang-Huei; Chang, Gwo-Jyh; Kuo, Chi-Tai

2011-03-01

Atrial fibrillation (AF) is a common complication in hyperthyroidism. Earlier studies demonstrate that thyroid hormone decreases L-type calcium channel (LCC) current expression with resultant shortening of action potential duration (APD), providing a substrate for AF. The aim of this study was to investigate the potential mechanism underlying the regulatory effect of thyroid hormone on LCC. In a hyperthyroid rat model, thyroid hormone (triiodothyronine [T3]) administration down-regulated atrial LCC expression. In vitro, treatment of murine atrial myocytes (HL-1) with T3 decreased the expression of LCC and its current, resulting in abbreviation of APD. Furthermore, T3 inhibited the activation of cyclic AMP response element (CRE)-binding protein (CREB), including phosphorylation at Ser133 and its nuclear translocation. Transient transfection studies in HL-1 cells indicated that T3 reduced LCC promoter activity. Deletion and mutation analysis of the LCC promoter region along with chromatin immunoprecipitation using anti-CREB antibody showed that CRE was essential for T3-mediated LCC gene expression. Transfection of dominant-negative CREB (mutated Ser133) and mutant thyroid hormone receptor (TR, mutated Cys51) abolished the T3-dependent effects, suggesting an association between both transcriptional factors. Co-immunoprecipitation documented an increased binding of TR with CREB after T3 treatment. The transcriptional cross-talk 3 between TR and CREB bound to CRE mediates T3-inhibited CREB activity and LCC expression. Thyroid hormone-induced TR binding of CREB inhibits CREB activity and LCC current expression, which may contribute to AF. These findings provide an important mechanistic insight into hyperthyroidism-induced AF.

NOAA tools to support CSC and LCC regional climate science priorities in the western Gulf of Mexico

NASA Astrophysics Data System (ADS)

Brown, D. P.; Marcy, D.; Robbins, K.; Shafer, M.; Stiller, H.

2012-12-01

The National Oceanic and Atmospheric Administration (NOAA) is an active regional partner with the Department of Interior (DOI) in supplying and supporting the delivery of climate science and services. A primary mechanism for NOAA-DOI coordination at the regional scale is the Landscape Conservation Cooperative (LCC) network, which is supported in part by DOI Climate Science Centers (CSC). Together, the CSCs and LCCs provide a framework to identify landscape-scale science and services priorities for conservation and management. As a key partner of the CSCs and an active member of many LCCs, NOAA is working to ensure its own regional product and service delivery efforts will help address these conservation and management challenges. Two examples of NOAA's regional efforts are highlighted here, with a focus on the coastal and interior geographies of the western Gulf of Mexico where NOAA partners with the South Central CSC and participates as a member of the Gulf Coast Prairie LCC. Along the Texas coastline, a sea level rise and coastal flooding impacts viewer, produced by NOAA's Coastal Services Center and available via its Digital Coast interface, allows constituents to visualize estimates of sea level rise, measures of uncertainty, flood frequencies, and environmental (e.g., marsh migration) and socioeconomic (e.g., tidal flooding of built environments) impacts. In the interior of Texas and Louisiana, NOAA's Southern Regional Climate Center is leading a consortium of partners in the development of a unified source of regional water reservoir information, including current conditions, a historical database, and web-based visualization tools to illustrate spatio-temporal variations in water availability to a broad array of hydrological, agricultural, and other customers. These two examples of NOAA products can, in their existing forms, support regional conservation and management priorities for CSCs and LCCs by informing vulnerability assessments and adaptation planning. Enhancements to these and other efforts can be achieved through a robust collaboration between NOAA and DOI that links regional science priorities to regional service delivery.

Recurrent neural network control for LCC-resonant ultrasonic motor drive.

PubMed

Lin, F J; Wai, R J; Hong, C M

2000-01-01

A newly designed driving circuit for the traveling wave-type ultrasonic motor (USM), which consists of a push-pull DC-DC power converter and a two-phase voltage source inverter using one inductance and two capacitances (LCC) resonant technique, is presented in this study. Moreover, because the dynamic characteristics of the USM are difficult to obtain and the motor parameters are time varying, a recurrent neural network (RNN) controller is proposed to control the USM drive system. In the proposed controller, the dynamic backpropagation algorithm is adopted to train the RNN on-line using the proposed delta adaptation law. Furthermore, to guarantee the convergence of tracking error, analytical methods based on a discrete-type Lyapunov function are proposed to determine the varied learning rates for the training of the RNN. Finally, the effectiveness of the RNN-controlled USM drive system is demonstrated by some experimental results.

Integration of RAMS in LCC analysis for linear transport infrastructures. A case study for railways.

NASA Astrophysics Data System (ADS)

Calle-Cordón, Álvaro; Jiménez-Redondo, Noemi; Morales-Gámiz, F. J.; García-Villena, F. A.; Garmabaki, Amir H. S.; Odelius, Johan

2017-09-01

Life-cycle cost (LCC) analysis is an economic technique used to assess the total costs associated with the lifetime of a system in order to support decision making in long term strategic planning. For complex systems, such as railway and road infrastructures, the cost of maintenance plays an important role in the LCC analysis. Costs associated with maintenance interventions can be more reliably estimated by integrating the probabilistic nature of the failures associated to these interventions in the LCC models. Reliability, Maintainability, Availability and Safety (RAMS) parameters describe the maintenance needs of an asset in a quantitative way by using probabilistic information extracted from registered maintenance activities. Therefore, the integration of RAMS in the LCC analysis allows obtaining reliable predictions of system maintenance costs and the dependencies of these costs with specific cost drivers through sensitivity analyses. This paper presents an innovative approach for a combined RAMS & LCC methodology for railway and road transport infrastructures being developed under the on-going H2020 project INFRALERT. Such RAMS & LCC analysis provides relevant probabilistic information to be used for condition and risk-based planning of maintenance activities as well as for decision support in long term strategic investment planning.

User’s Manual for SEEK TALK Full Scale Engineering Development Life Cycle Cost (LCC) Model. Volume II. Model Equations and Model Operations.

DTIC Science & Technology

1981-04-01

LIFE CYCLE COST (LCC) LCC SENSITIVITY ANALYSIS LCC MODE , REPAIR LEVEL ANALYSIS (RLA) 20 ABSTRACT (Cnn tlnue on reverse side It necessary and Identify... level analysis capability. Next it provides values for Air Force input parameters and instructions for contractor inputs, general operating...Maintenance Manhour Requirements 39 5.1.4 Calculation of Repair Level Fractions 43 5.2 Cost Element Equations 47 5.2.1 Production Cost Element 47

Anti-HIV and immunomodulation activities of cacao mass lignin-carbohydrate complex.

PubMed

Sakagami, Hiroshi; Kawano, Michiyo; Thet, May Maw; Hashimoto, Ken; Satoh, Kazue; Kanamoto, Taisei; Terakubo, Shigemi; Nakashima, Hideki; Haishima, Yuji; Maeda, Yuuichi; Sakurai, Koji

2011-01-01

Recently, a prominent antiviral and macrophage stimulatory activity of cacao lignin-carbohydrate complex (LCC) has been reported. However, the solubility and sterility of LCC have not been considered yet. In the present study, complete solubilisation and sterilisation was achieved by autoclaving under mild alkaline conditions and the previously reported biological activities were re-examined. LCCs were obtained by 1% NaOH extraction and acid precipitation, and a repeated extraction-precipitation cycle. Nitric oxide (NO) and cytokine productions were assayed by the Griess method and ELISA, respectively. Inducible NO synthase (iNOS) expression was determined by Western blot analysis. Superoxide anion, hydroxyl radical and nitric oxide radical-scavenging activity was determined by ESR spectroscopy. Cacao mass LCC showed reproducibly higher anti-HIV activity than cacao husk LCC. Cacao mass LCC, up to 62.5 μg/ml, did not stimulate mouse macrophage-like cells (RAW264.7 and J774.1) to produce NO, nor did it induce iNOS protein, in contrast to lipopolysaccharide (LPS). Cacao mass LCC and LPS synergistically stimulated iNOS protein expression, suggesting a different point of action. Cacao mass LCC induced tumour necrosis factor-α production markedly less than LPS, and did not induce interleukin-1β, interferon-α or interferon-γ. ESR spectroscopy showed that cacao mass LCC, but not LPS, scavenged NO produced from NOC-7. This study demonstrated several new biological activities of LCCs distinct from LPS and further confirmed the promising antiviral and immunomodulating activities of LCCs.

Use of Laccase as a Novel, Versatile Reporter System in Filamentous Fungi

PubMed Central

Mander, Gerd J.; Wang, Huaming; Bodie, Elizabeth; Wagner, Jens; Vienken, Kay; Vinuesa, Claudia; Foster, Caroline; Leeder, Abigail C.; Allen, Gethin; Hamill, Valerie; Janssen, Giselle G.; Dunn-Coleman, Nigel; Karos, Marvin; Lemaire, Hans Georg; Subkowski, Thomas; Bollschweiler, Claus; Turner, Geoffrey; Nüsslein, Bernhard; Fischer, Reinhard

2006-01-01

Laccases are copper-containing enzymes which oxidize phenolic substrates and transfer the electrons to oxygen. Many filamentous fungi contain several laccase-encoding genes, but their biological roles are mostly not well understood. The main interest in laccases in biotechnology is their potential to be used to detoxify phenolic substances. We report here on a novel application of laccases as a reporter system in fungi. We purified a laccase enzyme from the ligno-cellulolytic ascomycete Stachybotrys chartarum. It oxidized the artificial substrate 2,2′-azino-di-(3-ethylbenzthiazolinsulfonate) (ABTS). The corresponding gene was isolated and expressed in Aspergillus nidulans, Aspergillus niger, and Trichoderma reesei. Heterologously expressed laccase activity was monitored in colorimetric enzyme assays and on agar plates with ABTS as a substrate. The use of laccase as a reporter was shown in a genetic screen for the isolation of improved T. reesei cellulase production strains. In addition to the laccase from S. charatarum, we tested the application of three laccases from A. nidulans (LccB, LccC, and LccD) as reporters. Whereas LccC oxidized ABTS (Km = 0.3 mM), LccD did not react with ABTS but with DMA/ADBP (3,5-dimethylaniline/4-amino-2,6-dibromophenol). LccB reacted with DMA/ADBP and showed weak activity with ABTS. The different catalytic properties of LccC and LccD allow simultaneous use of these two laccases as reporters in one fungal strain. PMID:16820501

Characterization and cloning of laccase gene from Hericium coralloides NBRC 7716 suitable for production of epitheaflagallin 3-O-gallate.

PubMed

Itoh, Nobuya; Takagi, Shinya; Miki, Asami; Kurokawa, Junji

2016-01-01

Epitheaflagallin 3-O-gallate (ETFGg) is a minor polyphenol found in black tea extract, which has good physiological functions. It is synthesized from epigallocatechin gallate (EGCg) with gallic acid via laccase oxidation. Various basidiomycetes and fungi were screened to find a suitable laccase for the production of ETFGg. A basidiomycete, Hericium coralloides NBRC 7716, produced an appropriate extracellular laccase. The purified laccase produced twice the level of ETFGg compared with commercially available laccase from Trametes sp. The enzyme, termed Lcc2, is a monomeric protein with an apparent molecular mass of 67.2 kDa. The N-terminal amino acid sequence of Lcc2 is quite different from laccase isolated from the fruiting bodies of Hericium. Lcc2 showed similar substrate specificity to known laccases and could oxidize various phenolic substrates, including pyrogallol, gallic acid, and 2,6-dimethoxyphenol. The full-length lcc2 gene was obtained by PCR using degenerate primers, which were designed based on the N-terminal amino acid sequence of Lcc2 and conserved copper-binding sites of laccases, and 5'-, and 3'-RACE PCR with mRNA. The Lcc2 gene showed homology with Lentinula edodes laccase (sharing 77% amino acid identity with Lcc6). We successfully produced extracellular Lcc2 using a heterologous expression system with Saccharomyces cerevisiae. Moreover, it was confirmed that the recombinant laccase generates similar levels of ETFGg as the native enzyme. Copyright © 2015 Elsevier Inc. All rights reserved.

Use of laccase as a novel, versatile reporter system in filamentous fungi.

PubMed

Mander, Gerd J; Wang, Huaming; Bodie, Elizabeth; Wagner, Jens; Vienken, Kay; Vinuesa, Claudia; Foster, Caroline; Leeder, Abigail C; Allen, Gethin; Hamill, Valerie; Janssen, Giselle G; Dunn-Coleman, Nigel; Karos, Marvin; Lemaire, Hans Georg; Subkowski, Thomas; Bollschweiler, Claus; Turner, Geoffrey; Nüsslein, Bernhard; Fischer, Reinhard

2006-07-01

Laccases are copper-containing enzymes which oxidize phenolic substrates and transfer the electrons to oxygen. Many filamentous fungi contain several laccase-encoding genes, but their biological roles are mostly not well understood. The main interest in laccases in biotechnology is their potential to be used to detoxify phenolic substances. We report here on a novel application of laccases as a reporter system in fungi. We purified a laccase enzyme from the ligno-cellulolytic ascomycete Stachybotrys chartarum. It oxidized the artificial substrate 2,2'-azino-di-(3-ethylbenzthiazolinsulfonate) (ABTS). The corresponding gene was isolated and expressed in Aspergillus nidulans, Aspergillus niger, and Trichoderma reesei. Heterologously expressed laccase activity was monitored in colorimetric enzyme assays and on agar plates with ABTS as a substrate. The use of laccase as a reporter was shown in a genetic screen for the isolation of improved T. reesei cellulase production strains. In addition to the laccase from S. charatarum, we tested the application of three laccases from A. nidulans (LccB, LccC, and LccD) as reporters. Whereas LccC oxidized ABTS (Km = 0.3 mM), LccD did not react with ABTS but with DMA/ADBP (3,5-dimethylaniline/4-amino-2,6-dibromophenol). LccB reacted with DMA/ADBP and showed weak activity with ABTS. The different catalytic properties of LccC and LccD allow simultaneous use of these two laccases as reporters in one fungal strain.

New Approaches in Reusable Booster System Life Cycle Cost Modeling

NASA Technical Reports Server (NTRS)

Zapata, Edgar

2013-01-01

This paper presents the results of a 2012 life cycle cost (LCC) study of hybrid Reusable Booster Systems (RBS) conducted by NASA Kennedy Space Center (KSC) and the Air Force Research Laboratory (AFRL). The work included the creation of a new cost estimating model and an LCC analysis, building on past work where applicable, but emphasizing the integration of new approaches in life cycle cost estimation. Specifically, the inclusion of industry processes/practices and indirect costs were a new and significant part of the analysis. The focus of LCC estimation has traditionally been from the perspective of technology, design characteristics, and related factors such as reliability. Technology has informed the cost related support to decision makers interested in risk and budget insight. This traditional emphasis on technology occurs even though it is well established that complex aerospace systems costs are mostly about indirect costs, with likely only partial influence in these indirect costs being due to the more visible technology products. Organizational considerations, processes/practices, and indirect costs are traditionally derived ("wrapped") only by relationship to tangible product characteristics. This traditional approach works well as long as it is understood that no significant changes, and by relation no significant improvements, are being pursued in the area of either the government acquisition or industry?s indirect costs. In this sense then, most launch systems cost models ignore most costs. The alternative was implemented in this LCC study, whereby the approach considered technology and process/practices in balance, with as much detail for one as the other. This RBS LCC study has avoided point-designs, for now, instead emphasizing exploring the trade-space of potential technology advances joined with potential process/practice advances. Given the range of decisions, and all their combinations, it was necessary to create a model of the original model and use genetic algorithms to explore results. A strong business case occurs when viable paths are identified for an affordable up-front investment, and these paths can credibly achieve affordable, responsive operations, characterized by smaller direct touch labor efforts at the wing level from flight to flight. The results supporting this approach, its potential, and its conclusions are presented here.

New Approaches in Reuseable Booster System Life Cycle Cost Modeling

NASA Technical Reports Server (NTRS)

Zapata, Edgar

2013-01-01

This paper presents the results of a 2012 life cycle cost (LCC) study of hybrid Reusable Booster Systems (RBS) conducted by NASA Kennedy Space Center (KSC) and the Air Force Research Laboratory (AFRL). The work included the creation of a new cost estimating model and an LCC analysis, building on past work where applicable, but emphasizing the integration of new approaches in life cycle cost estimation. Specifically, the inclusion of industry processes/practices and indirect costs were a new and significant part of the analysis. The focus of LCC estimation has traditionally been from the perspective of technology, design characteristics, and related factors such as reliability. Technology has informed the cost related support to decision makers interested in risk and budget insight. This traditional emphasis on technology occurs even though it is well established that complex aerospace systems costs are mostly about indirect costs, with likely only partial influence in these indirect costs being due to the more visible technology products. Organizational considerations, processes/practices, and indirect costs are traditionally derived ("wrapped") only by relationship to tangible product characteristics. This traditional approach works well as long as it is understood that no significant changes, and by relation no significant improvements, are being pursued in the area of either the government acquisition or industry?s indirect costs. In this sense then, most launch systems cost models ignore most costs. The alternative was implemented in this LCC study, whereby the approach considered technology and process/practices in balance, with as much detail for one as the other. This RBS LCC study has avoided point-designs, for now, instead emphasizing exploring the trade-space of potential technology advances joined with potential process/practice advances. Given the range of decisions, and all their combinations, it was necessary to create a model of the original model and use genetic algorithms to explore results. A strong business case occurs when viable paths are identified for an affordable up-front investment, and these paths can credibly achieve affordable, responsive operations, characterized by smaller direct touch labor efforts at the wing level from flight to flight. The results supporting this approach, its potential, and its conclusions are presented here.

Induction of lcc2 expression and activity by Agaricus bisporus provides defence against Trichoderma aggressivum toxic extracts

PubMed Central

Sjaarda, Calvin P; Abubaker, Kamal S; Castle, Alan J

2015-01-01

Laccases are used by fungi for several functions including defence responses to stresses associated with attack by other fungi. Laccase activity changes and the induction of two laccase genes, lcc1 and lcc2, in Agaricus bisporus were measured in response to toxic extracts of medium in which Trichoderma aggressivum, the cause of green mould disease, was grown. A strain of A. bisporus that shows resistance to the extracts showed higher basal levels and greater enzymatic activity after extract exposure than did a sensitive strain. Furthermore, pre-incubation of T. aggressivum extract with laccases reduced toxicity. Faster induction and greater numbers of lcc2 transcripts in response to the extract were noted in the resistant strain than in the sensitive strain. The timing and increase in lcc2 transcript abundance mirrored changes in total laccase activity. No correlation between resistance and lcc1 transcription was apparent. Transcript abundance in transformants with a siRNA construct homologous to both genes varied widely. A strong negative correlation between transcript abundance and sensitivity of the transformant to toxic extract was observed in plate assays. These results indicated that laccase activity and in particular that encoded by lcc2 contributes to toxin metabolism and by extension green mould disease resistance. PMID:25824278

On the spread of changes in marine low cloud cover in climate model simulations of the 21st century

NASA Astrophysics Data System (ADS)

Qu, Xin; Hall, Alex; Klein, Stephen A.; Caldwell, Peter M.

2014-05-01

In 36 climate change simulations associated with phases 3 and 5 of the Coupled Model Intercomparison Project (CMIP3 and CMIP5), changes in marine low cloud cover (LCC) exhibit a large spread, and may be either positive or negative. Here we develop a heuristic model to understand the source of the spread. The model's premise is that simulated LCC changes can be interpreted as a linear combination of contributions from factors shaping the clouds' large-scale environment. We focus primarily on two factors—the strength of the inversion capping the atmospheric boundary layer (measured by the estimated inversion strength, EIS) and sea surface temperature (SST). For a given global model, the respective contributions of EIS and SST are computed. This is done by multiplying (1) the current-climate's sensitivity of LCC to EIS or SST variations, by (2) the climate-change signal in EIS or SST. The remaining LCC changes are then attributed to changes in greenhouse gas and aerosol concentrations, and other environmental factors. The heuristic model is remarkably skillful. Its SST term dominates, accounting for nearly two-thirds of the intermodel variance of LCC changes in CMIP3 models, and about half in CMIP5 models. Of the two factors governing the SST term (the SST increase and the sensitivity of LCC to SST perturbations), the SST sensitivity drives the spread in the SST term and hence the spread in the overall LCC changes. This sensitivity varies a great deal from model to model and is strongly linked to the types of cloud and boundary layer parameterizations used in the models. EIS and SST sensitivities are also estimated using observational cloud and meteorological data. The observed sensitivities are generally consistent with the majority of models as well as expectations from prior research. Based on the observed sensitivities and the relative magnitudes of simulated EIS and SST changes (which we argue are also physically reasonable), the heuristic model predicts LCC will decrease over the 21st-century. However, to place a strong constraint, for example on the magnitude of the LCC decrease, will require longer observational records and a careful assessment of other environmental factors producing LCC changes. Meanwhile, addressing biases in simulated EIS and SST sensitivities will clearly be an important step towards reducing intermodel spread in simulated LCC changes.

ASCANS Saturn V & LCC Tour

NASA Image and Video Library

2014-03-03

CAPE CANAVERAL, Fla. – NASA astronaut candidates Victor Glover, from left, Andrew Morgan and Jessica Meir tour the Apollo Saturn V Center at Kennedy Space Center in Florida during a daylong set of briefings and tours of different facilities at NASA's primary launch center. The astronaut class of 2013 was selected by NASA after an extensive year-and-a-half search. The new group will help the agency push the boundaries of exploration and travel to new destinations in the solar system. To learn more about the astronaut class of 2013, visit: http://www.nasa.gov/astronauts/2013astroclass.html Photo credit: NASA/Kim Shiflett

Chlorophyll dynamic accounts for spatial and temporal variabilities in terrestrial carbon uptake and evapotranspiration

NASA Astrophysics Data System (ADS)

Croft, H.; Luo, X.; Chen, J. M.

2017-12-01

Terrestrial carbon and water fluxes are driven by a range of abiotic and biotic factors. State-of-art terrestrial biosphere models (TBMs) use numerical representations of these factors in conjunction with concise descriptions of biogeochemical processes to estimate terrestrial fluxes (i.e. gross primary productivity (GPP) and evapotranspiration(ET)). Leaf maximum carboxylation rate (Vcmax25) is a key biotic factor prescribed in TBM to determine CO2 assimilation rates and leaf stomatal conductivity for water transport, but the paucity of its measurements has long plagued the simulation of fluxes. This study uses leaf chlorophyll content (LCC) derived from remotely sensed data to account for spatial and temporal variations in Vcmax25 within a TBM framework. Results from the TBM with and without LCC were validated against measurements from 124 eddy-covariance towers (554 site-years) of FLUXNET. TBM using LCC reduced the biases of estimated GPP in 61% of the site-years and 59% for ET, with especially large improvements for biomes with strong seasonal cycles (e.g. deciduous forest, croplands and grasslands). In addition to the Vcmax25 adjustment imposed by LCC seasonal patterns, the spatial variability of LCC acts as an equally important part in reducing the errors of estimated fluxes by capturing the spatial variations of Vcmax25, especially during the summer. This study presents the first case of integrating satellite-derived LCC into a TBM at the global scale. Our results demonstrate the critical role of LCC in describing the variabilities in the terrestrial carbon uptake and ET and the necessity of including LCC in future TBMs.

Spaceport Command and Control System Support Software Development

NASA Technical Reports Server (NTRS)

Brunotte, Leonard

2016-01-01

The Spaceport Command and Control System (SCCS) is a project developed and used by NASA at Kennedy Space Center in order to control and monitor the Space Launch System (SLS) at the time of its launch. One integral subteam under SCCS is the one assigned to the development of a data set building application to be used both on the launch pad and in the Launch Control Center (LCC) at the time of launch. This web application was developed in Ruby on Rails, a web framework using the Ruby object-oriented programming language, by a 15 - employee team (approx.). Because this application is such a huge undertaking with many facets and iterations, there were a few areas in which work could be more easily organized and expedited. As an intern working with this team, I was charged with the task of writing web applications that fulfilled this need, creating a virtual and highly customizable whiteboard in order to allow engineers to keep track of build iterations and their status. Additionally, I developed a knowledge capture web application wherein any engineer or contractor within SCCS could ask a question, answer an existing question, or leave a comment on any question or answer, similar to Stack Overflow.

Serum DHCR24 Auto-antibody as a new Biomarker for Progression of Hepatitis C

PubMed Central

Ezzikouri, Sayeh; Kimura, Kiminori; Sunagozaka, Hajime; Kaneko, Shuichi; Inoue, Kazuaki; Nishimura, Tomohiro; Hishima, Tsunekazu; Kohara, Michinori; Tsukiyama-Kohara, Kyoko

2015-01-01

Background New biomarkers are needed to identify the stage of hepatitis C virus (HCV)-infected diseases in order to reduce the mortality rates. Herein, we investigated whether serum 3β-hydroxysterol Δ24-reductase antibody (DHCR24 Ab) may serve as a prognostic marker for hepatitis C infection progression to hepatocellular carcinoma (HCC). Methods Serum DHCR24 Abs from 395 HCV-positive patients, including 133 chronic hepatitis (CHC), 85 liver cirrhosis (LCC), and 177 HCC (HCC-C) patients; 232 hepatitis B virus (HBV)-positive patients, including 103 chronic hepatitis (CHB), 56 liver cirrhosis (LCB), and 73 HCC (HCC-B) patients; and 24 healthy controls, were measured using enzyme-linked immunosorbent assay. Results The serum DHCR24 Ab levels were significantly higher in patients with CHC than in healthy controls, in LCC than in CHC, and in LCC than in HCC-C (P < 0.0001 for all). The concentration of serum DHCR24 Ab in HCC-B patients showed no significant difference compared to CHB and LCB patients (P = 0.1247). The DHCR24 Ab levels were significantly higher in early HCC-C than CHC or LCC patients and in late HCC-C compared to early HCC-C patients. The sensitivity of the DHCR24 Ab for HCC-C detection (70.6%) was higher than that of alpha-fetoprotein (AFP; 54.8%) and protein induced by vitamin K absence or antagonist-II (PIVKA-II; 42 · 5%). Moreover, DHCR24 was up-regulated in HCV-positive, but not HBV-positive tissues or HBV-negative, HCV-negative HCC specimens. Conclusions DHCR24 auto-antibody represents a potential noninvasive biomarker for HCV-related liver disease and may facilitate the diagnosis of PIVKA-II and AFP-negative HCC. PMID:26288822

Computerized LCC/ORLA methodology. [Life cycle cost/optimum repair level analysis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Henderson, J.T.

1979-01-01

The effort by Sandia Laboratories in developing CDC6600 computer programs for Optimum Repair Level Analysis (ORLA) and Life Cycle Cost (LCC) analysis is described. Investigation of the three repair-level strategies referenced in AFLCM/AFSCM 800-4 (base discard of subassemblies, base repair of subassemblies, and depot repair of subassemblies) was expanded to include an additional three repair-level strategies (base discard of complete assemblies and, upon shipment of complete assemblies to the depot, depot repair of assemblies by subassembly repair, and depot repair of assemblies by subassembly discard). The expanded ORLA was used directly in an LCC model that was procedurally altered tomore » accommodate the ORLA input data. Available from the LCC computer run was an LCC value corresponding to the strategy chosen from the ORLA. 2 figures.« less

Physical Characterization of Gemini Surfactant-Based Synthetic Vectors for the Delivery of Linear Covalently Closed (LCC) DNA Ministrings

PubMed Central

Sum, Chi Hong; Nafissi, Nafiseh; Slavcev, Roderick A.; Wettig, Shawn

2015-01-01

In combination with novel linear covalently closed (LCC) DNA minivectors, referred to as DNA ministrings, a gemini surfactant-based synthetic vector for gene delivery has been shown to exhibit enhanced delivery and bioavailability while offering a heightened safety profile. Due to topological differences from conventional circular covalently closed (CCC) plasmid DNA vectors, the linear topology of LCC DNA ministrings may present differences with regards to DNA interaction and the physicochemical properties influencing DNA-surfactant interactions in the formulation of lipoplexed particles. In this study, N,N-bis(dimethylhexadecyl)-α,ω-propanediammonium(16-3-16)gemini-based synthetic vectors, incorporating either CCC plasmid or LCC DNA ministrings, were characterized and compared with respect to particle size, zeta potential, DNA encapsulation, DNase sensitivity, and in vitro transgene delivery efficacy. Through comparative analysis, differences between CCC plasmid DNA and LCC DNA ministrings led to variations in the physical properties of the resulting lipoplexes after complexation with 16-3-16 gemini surfactants. Despite the size disparities between the plasmid DNA vectors (CCC) and DNA ministrings (LCC), differences in DNA topology resulted in the generation of lipoplexes of comparable particle sizes. The capacity for ministring (LCC) derived lipoplexes to undergo complete counterion release during lipoplex formation contributed to improved DNA encapsulation, protection from DNase degradation, and in vitro transgene delivery. PMID:26561857

Selected Logistics Models and Techniques.

DTIC Science & Technology

1984-09-01

TI - 59 Programmable Calculator LCC...Program 27 TI - 59 Programmable Calculator LCC Model 30 Unmanned Spacecraft Cost Model 31 iv I: TABLE OF CONTENTS (CONT’D) (Subject Index) LOGISTICS...34"" - % - "° > - " ° .° - " .’ > -% > ]*° - LOGISTICS ANALYSIS MODEL/TECHNIQUE DATA MODEL/TECHNIQUE NAME: TI - 59 Programmable Calculator LCC Model TYPE MODEL: Cost Estimating DEVELOPED BY:

STS-99 Flight Day Highlights and Crew Activities Report

NASA Technical Reports Server (NTRS)

2000-01-01

Live footage shows the Blue Team (second of the dual shift crew), Dominic L. Pudwill Gorie, Janice E. Voss and Mamoru Mohri, beginning the first mapping swath covering a 140-mile-wide path. While Mohri conducts mapping operations, Voss and Gorie are seen participating in a news conference with correspondents from NBC and CNN. The Red Team (first of the dual shift crew), Kevin R. Kregel, Janet L. Kavandi and Gerhard P.J. Thiele, relieves the Blue Team and are seen continuing the mapping operations for this around the clock Shuttle Radar Topography Mission (SRTM). Commander Kregel is shown performing boom (mass) durability tests, calibrating the EarthCam Payload, and speaking with the Launch Control Center (LCC) about trouble shooting a bracket for better camera angle.

Comparative Research to Surface Aeration and Blasting Aeration System Based on LCC Theory

NASA Astrophysics Data System (ADS)

Liai, CHEN; Hongxun, HOU; Weibiao, FEI; Eryan, ZHAO

2017-05-01

It is difficult to select the suitable aeration system for the designers of wastewater treatment plant (WWTP). In this paper, taking two WWTPs with surface aeration systems and blasting aeration respectively for an example, LCC theory was adapted to analysis the cost of consumption and the environmental impact, which caused by the different aeration system. Research results showed that: (1) In the 20-year life cycle, the LCC mainly depended on the cost of energy consumption whatever blasting aeration system or surface aeration, while the LCC of blasting aeration system affected by the equipment maintenance cost, maintenance cost, economic losses caused by wastewater loss and environmental load in maintenance period. (2) The LCC of blasting aeration system was lower than the surface aeration in general, on the premise of the standard discharge; (3) the blasting aeration system estimated a saving of 60,0000RMB annually in costs compared with the surface aeration.

Navy Program Manager’s Guide, 1985 Edition

DTIC Science & Technology

1985-01-01

1-7 Relationship of Development Cost in System Life -Cycle Cost (LCC) ......................... 1-7 Realistic Costing and Budgeting...Review (PROR)..... 4-53 x MI *) First-Article Configuration Inspection (FACI) ...... 4-54 Cost Management- Life -Cycle Costing (LCC) ..................... 4...innovation and minimize costs. 4. Consideration of life -cycle cost (LCC) such that affordability is put on an equal basis with system performance, schedule

Using No-Stakes Educational Testing to Mitigate Summer Learning Loss: A Pilot Study. Research Report. ETS RR-14-21

ERIC Educational Resources Information Center

Zaromb, Franklin; Adler, Rachel M.; Bruce, Kelly; Attali, Yigal; Rock, JoAnn

2014-01-01

This study investigates the benefits of no-stakes educational testing during students' summer vacation as a strategy to mitigate summer learning loss. Fifty-one students in Grades 3-8 from the Every Child Valued (ECV) and Lawrence Community Center (LCC) summer programs in Lawrenceville, NJ, took short, online assessments throughout the summer,…

ASCANS Saturn V & LCC Tour

NASA Image and Video Library

2014-03-03

CAPE CANAVERAL, Fla. – NASA astronaut candidates Andrew Morgan, from left, Victor Glover, Josh Cassada, Anne McClain and Jessica Meir tour the Apollo Saturn V Center at Kennedy Space Center in Florida during a daylong set of briefings and tours of different facilities at NASA's primary launch center. The astronaut class of 2013 was selected by NASA after an extensive year-and-a-half search. The new group will help the agency push the boundaries of exploration and travel to new destinations in the solar system. To learn more about the astronaut class of 2013, visit: http://www.nasa.gov/astronauts/2013astroclass.html Photo credit: NASA/Kim Shiflett

Electro-optical properties of the metal oxide-carbon thin film system of CdO-LCC

NASA Astrophysics Data System (ADS)

Kokshina, A. V.; Smirnov, A. V.; Razina, A. G.

2016-08-01

This article presents the results of a study electrical and optical properties of the thin film system of CdO-LCC. Cadmium oxide films were obtained by method of thermal oxidation. CdO-LCC thin film system was produced by applying on a CdO film a linear chain carbon film in thickness of 100 nm using the ion-plasma method, after which the obtained system was annealed. The studies showed that the obtained CdO-LCC films are quite transparent in the visible region; it has polycrystalline structure, thickness around 300 nm, the band gap to 2.3 eV. The obtained thin film system has photosensitive properties.

Microstructure and physical properties of steel-ladle purging plug refractory materials

NASA Astrophysics Data System (ADS)

Long, Bin; Xu, Gui-ying; Andreas, Buhr

2017-02-01

Three different castables were prepared as steel-ladle purging-plug refractory materials: corundum-based low-cement castable (C-LCC), corundum-spinel-based low-cement castable (C-S-LCC), and no-cement corundum-spinel castable (C-S-NCC) (hydratable alumina ρ-Al2O3 bonded). The properties of these castables were characterized with regard to water demand/flow ability, cold crushing strength (CCS), cold modulus of rupture (CMoR), permanent linear change (PLC), apparent porosity, and hot modulus of rupture (HMoR). The results show the CCS/CMoR and HMoR of C-LCC and C-S-LCC are greater than those of the castable C-S-NCC. According to the microstructure analysis, the sintering effect and the bonding type of the matrix material differ among the three castables. The calcium hexaluminate (CA6) phase in the matrix of C-LCC enhances the cold and hot mechanical strengths. In the case of C-S-LCC, the CA6 and 2CaO·2MgO·14Al2O3 (C2M2A14) ternary phases generated from the matrix can greatly increase the cold and hot mechanical strengths. In the case of the no-cement castable, sintering becomes difficult, resulting in a lower mechanical strength.

Completion of Launch Director Console Project and Other Support Work

NASA Technical Reports Server (NTRS)

Steinrock, Joshua G.

2018-01-01

There were four projects that I was a part of working on during the spring semester of 2018. This included the completion of the Launch Director Console (LDC) project and the completion and submission of a Concept of Operations (ConOps) document for the Record and Playback System (RPS) at the Launch Control Center (LCC), as well as supporting the implementation of a unit in RPS known as the CDP (Communication Data Processor). Also included was my support and mentorship of a High School robotics team that is sponsored by Kennedy Space Center. The LDC project is an innovative workstation to be used by the launch director for the future Space Launch System program. I worked on the fabrication and assembly of the final console. The ConOps on RPS is a technical document for which I produced supporting information and notes. All of this was done in the support of the IT Project Management Office (IT-F). The CDP is a subsystem that will eventually be installed in and operated by RPS.

LccA, an Archaeal Laccase Secreted as a Highly Stable Glycoprotein into the Extracellular Medium by Haloferax volcanii▿ †

PubMed Central

Uthandi, Sivakumar; Saad, Boutaiba; Humbard, Matthew A.; Maupin-Furlow, Julie A.

2010-01-01

Laccases couple the oxidation of phenolic compounds to the reduction of molecular oxygen and thus span a wide variety of applications. While laccases of eukaryotes and bacteria are well characterized, these enzymes have not been described in archaea. Here, we report the purification and characterization of a laccase (LccA) from the halophilic archaeon Haloferax volcanii. LccA was secreted at high levels into the culture supernatant of a recombinant H. volcanii strain, with peak activity (170 ± 10 mU·ml−1) at stationary phase (72 to 80 h). LccA was purified 13-fold to an overall yield of 72% and a specific activity of 29.4 U·mg−1 with an absorbance spectrum typical of blue multicopper oxidases. The mature LccA was processed to expose an N-terminal Ala after the removal of 31 amino acid residues and was glycosylated to 6.9% carbohydrate content. Purified LccA oxidized a variety of organic substrates, including bilirubin, syringaldazine (SGZ), 2,2,-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and dimethoxyphenol (DMP), with DMP oxidation requiring the addition of CuSO4. Optimal oxidation of ABTS and SGZ was at 45°C and pH 6 and pH 8.4, respectively. The apparent Km values for SGZ, bilirubin, and ABTS were 35, 236, and 670 μM, with corresponding kcat values of 22, 29, and 10 s−1, respectively. The purified LccA was tolerant of high salt, mixed organosolvents, and high temperatures, with a half-life of inactivation at 50°C of 31.5 h. PMID:19966030

Life cycle costing of food waste: A review of methodological approaches.

PubMed

De Menna, Fabio; Dietershagen, Jana; Loubiere, Marion; Vittuari, Matteo

2018-03-01

Food waste (FW) is a global problem that is receiving increasing attention due to its environmental and economic impacts. Appropriate FW prevention, valorization, and management routes could mitigate or avoid these effects. Life cycle thinking and approaches, such as life cycle costing (LCC), may represent suitable tools to assess the sustainability of these routes. This study analyzes different LCC methodological aspects and approaches to evaluate FW management and valorization routes. A systematic literature review was carried out with a focus on different LCC approaches, their application to food, FW, and waste systems, as well as on specific methodological aspects. The review consisted of three phases: a collection phase, an iterative phase with experts' consultation, and a final literature classification. Journal papers and reports were retrieved from selected databases and search engines. The standardization of LCC methodologies is still in its infancy due to a lack of consensus over definitions and approaches. Research on the life cycle cost of FW is limited and generally focused on FW management, rather than prevention or valorization of specific flows. FW prevention, valorization, and management require a consistent integration of LCC and Life Cycle Assessment (LCA) to avoid tradeoffs between environmental and economic impacts. This entails a proper investigation of methodological differences between attributional and consequential modelling in LCC, especially with regard to functional unit, system boundaries, multi-functionality, included cost, and assessed impacts. Further efforts could also aim at finding the most effective and transparent categorization of costs, in particular when dealing with multiple stakeholders sustaining costs of FW. Interpretation of results from LCC of FW should take into account the effect on larger economic systems. Additional key performance indicators and analytical tools could be included in consequential approaches. Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.

‘Sweeter Than a Swisher’: amount and themes of little cigar and cigarillo content on Twitter

PubMed Central

Kostygina, Ganna; Tran, Hy; Shi, Yaru; Kim, Yoonsang; Emery, Sherry

2016-01-01

Objective Despite recent increases in little cigar and cigarillo (LCC) use—particularly among urban youth, African-Americans and Latinos—research on targeted strategies for marketing these products is sparse. Little is known about the amount or content of LCC messages users see or share on social media, a popular communication medium among youth and communities of colour. Methods Keyword rules were used to collect tweets related to LCCs from the Twitter Firehose posted in October 2014 and March–April 2015. Tweets were coded for promotional content, brand references, co-use with marijuana and subculture references (eg, rap/hip-hop, celebrity endorsements) and were classified as commercial and ‘organic’/non-commercial using a combination of machine learning methods, keyword algorithms and human coding. Metadata associated with each tweet were used to categorise users as influencers (1000 and more followers) and regular users (under 1000 followers). Results Keyword filters captured over 4 372 293 LCC tweets. Analyses revealed that 17% of account users posting about LCCs were influencers and 1% of accounts were overtly commercial. Influencers were more likely to mention LCC brands and post promotional messages. Approximately 83% of LCC tweets contained references to marijuana and 29% of tweets were memes. Tweets also contained references to rap/hip-hop lyrics and urban subculture. Conclusions Twitter is a major information-sharing and marketing platform for LCCs. Co-use of tobacco and marijuana is common and normalised on Twitter. The presence and broad reach of LCC messages on social media warrants urgent need for surveillance and serious attention from public health professionals and policymakers. Future tobacco use prevention initiatives should be adapted to ensure that they are inclusive of LCC use. PMID:27697951

Nationwide surveillance of circulatory collapse associated with levothyroxine administration in very-low-birthweight infants in Japan.

PubMed

Kawai, Masahiko; Kusuda, Satoshi; Cho, Kazutoshi; Horikawa, Reiko; Takizawa, Fumihiko; Ono, Makoto; Hattori, Tetsuo; Oshiro, Makoto

2012-04-01

Although the administration of levothyroxine sodium (LT4) to premature infants had been considered safe, several cases of late-onset circulatory collapse (LCC) following the administration of LT4 in very-low-birth-weight (VLBW) infants have been reported in Japan since 2008. This study was performed to investigate the incidence of LCC associated with the administration of LT4 to VLBW infants. A questionnaire regarding LCC with or without an association with LT4 administration in VLBW infants from 2006 to 2008, was sent to 212 hospitals belonging to the Japan Neonatologist Association. Data of 8727 VLBW infants were analyzed, and 46 cases of LCC associated with the administration of LT4 were reported in this surveillance. Especially, an analysis for infants weighing between 1000 and 1499 g at birth revealed that the incidence of LCC with the administration of LT4 was higher than that of those without LT4. LT4 is widely used for infants, including VLBW infants, and no major complications have been reported. However, our study revealed that more than a few cases of LCC were associated with the administration of LT4 in VLBW infants. In conclusion, careful attention is necessary when initiating the administration of LT4 to VLBW infants. © 2011 The Authors. Pediatrics International © 2011 Japan Pediatric Society.

Classification Schedules as Subject Enhancement in Online Catalogs. A Review of a Conference Sponsored by Forest Press, the OCLC Online Computer Library Center, and the Council on Library Resources.

ERIC Educational Resources Information Center

Mandel, Carol A.

This paper presents a synthesis of the ideas and issues developed at a conference convened to review the results of the Dewey Decimal Classification Online Project and explore the potential for future use of the Dewey Decimal Classification (DDC) and Library of Congress Classification (LCC) schedules in online library catalogs. Conference…

Laccase Gene Expression and Vinasse Biodegradation by Trametes hirsuta Strain Bm-2.

PubMed

Tapia-Tussell, Raúl; Pérez-Brito, Daisy; Torres-Calzada, Claudia; Cortés-Velázquez, Alberto; Alzate-Gaviria, Liliana; Chablé-Villacís, Rubí; Solís-Pereira, Sara

2015-08-19

Vinasse is the dark-colored wastewater that is generated by bioethanol distilleries from feedstock molasses. The vinasse that is generated from molasses contains high amounts of pollutants, including phenolic compounds and melanoindin. The goal of this work was to study the expression of laccase genes in the Trametes hirsuta strain Bm-2, isolated in Yucatan, Mexico, in the presence of phenolic compounds, as well as its effectiveness in removing colorants from vinasse. In the presence of all phenolic compounds tested (guaiacol, ferulic acid, and vanillic acid), increased levels of laccase-encoding mRNA were observed. Transcript levels in the presence of guaiacol were 40 times higher than those in the control. The lcc1 and lcc2 genes of T. hirsuta were differentially expressed; guaiacol and vanillin induced the expression of both genes, whereas ferulic acid only induced the expression of lcc2. The discoloration of vinasse was concomitant with the increase in laccase activity. The highest value of enzyme activity (2543.7 U/mL) was obtained in 10% (v/v) vinasse, which corresponded to a 69.2% increase in discoloration. This study demonstrates the potential of the Bm-2 strain of T. hirsuta for the biodegradation of vinasse.

Space Transportation System Availability Relationships to Life Cycle Cost

NASA Technical Reports Server (NTRS)

Rhodes, Russel E.; Donahue, Benjamin B.; Chen, Timothy T.

2009-01-01

Future space transportation architectures and designs must be affordable. Consequently, their Life Cycle Cost (LCC) must be controlled. For the LCC to be controlled, it is necessary to identify all the requirements and elements of the architecture at the beginning of the concept phase. Controlling LCC requires the establishment of the major operational cost drivers. Two of these major cost drivers are reliability and maintainability, in other words, the system's availability (responsiveness). Potential reasons that may drive the inherent availability requirement are the need to control the number of unique parts and the spare parts required to support the transportation system's operation. For more typical space transportation systems used to place satellites in space, the productivity of the system will drive the launch cost. This system productivity is the resultant output of the system availability. Availability is equal to the mean uptime divided by the sum of the mean uptime plus the mean downtime. Since many operational factors cannot be projected early in the definition phase, the focus will be on inherent availability which is equal to the mean time between a failure (MTBF) divided by the MTBF plus the mean time to repair (MTTR) the system. The MTBF is a function of reliability or the expected frequency of failures. When the system experiences failures the result is added operational flow time, parts consumption, and increased labor with an impact to responsiveness resulting in increased LCC. The other function of availability is the MTTR, or maintainability. In other words, how accessible is the failed hardware that requires replacement and what operational functions are required before and after change-out to make the system operable. This paper will describe how the MTTR can be equated to additional labor, additional operational flow time, and additional structural access capability, all of which drive up the LCC. A methodology will be presented that provides the decision makers with the understanding necessary to place constraints on the design definition. This methodology for the major drivers will determine the inherent availability, safety, reliability, maintainability, and the life cycle cost of the fielded system. This methodology will focus on the achievement of an affordable, responsive space transportation system. It is the intent of this paper to not only provide the visibility of the relationships of these major attribute drivers (variables) to each other and the resultant system inherent availability, but also to provide the capability to bound the variables, thus providing the insight required to control the system's engineering solution. An example of this visibility is the need to provide integration of similar discipline functions to allow control of the total parts count of the space transportation system. Also, selecting a reliability requirement will place a constraint on parts count to achieve a given inherent availability requirement, or require accepting a larger parts count with the resulting higher individual part reliability requirements. This paper will provide an understanding of the relationship of mean repair time (mean downtime) to maintainability (accessibility for repair), and both mean time between failure (reliability of hardware) and the system inherent availability.

Long Non-Coding RNA (lncRNA) Urothelial Carcinoma-Associated 1 (UCA1) Enhances Tamoxifen Resistance in Breast Cancer Cells via Inhibiting mTOR Signaling Pathway.

PubMed

Wu, Chihua; Luo, Jing

2016-10-21

BACKGROUND Long non-coding RNA (lncRNA) UCA1 is an oncogene in breast cancer. The purpose of this study was to investigate the role of UCA1 in tamoxifen resistance of estrogen receptor positive breast cancer cells. MATERIAL AND METHODS Tamoxifen sensitive MCF-7 cells were transfected for UCA1 overexpression, while tamoxifen resistant LCC2 and LCC9 cells were transfected with UCA siRNA for UCA1 knockdown. qRT-PCR was performed to analyze UCA1 expression. CCK-8 assay, immunofluorescence staining of cleaved caspase-9, and flow cytometric analysis of Annexin V/PI staining were used to assess tamoxifen sensitivity. Western blot analysis was performed to detect p-AKT and p-mTOR expression. RESULTS LncRNA UCA1 was significantly upregulated in tamoxifen resistant breast cancer cells compared to tamoxifen sensitive cells. LCC2 and LCC9 cells transfected with UCA1 siRNA had significantly higher ratio of apoptosis after tamoxifen treatment. UCA1 siRNA significantly decreased the protein levels of p-AKT and p-mTOR in LCC2 and LCC9 cells. Enforced UCA1 expression substantially reduced tamoxifen induced apoptosis in MCF-7 cells, while rapamycin treatment abrogated the protective effect of UCA1. CONCLUSIONS UCA1 upregulation was associated with tamoxifen resistance in breast cancer. Mechanistically, UCA1 confers tamoxifen resistance to breast cancer cells partly via activating the mTOR signaling pathway.

LEUKOCYTE COPING CAPACITY AS A TOOL TO ASSESS CAPTURE- AND HANDLING-INDUCED STRESS IN SCANDINAVIAN BROWN BEARS (URSUS ARCTOS).

PubMed

Esteruelas, Núria Fandos; Huber, Nikolaus; Evans, Alina L; Zedrosser, Andreas; Cattet, Marc; Palomares, Francisco; Angel, Martine; Swenson, Jon E; Arnemo, Jon M

2016-04-01

Brown bears (Ursus arctos) are often captured and handled for research and management purposes. Although the techniques used are potentially stressful for the animals and might have detrimental and long-lasting consequences, it is difficult to assess their physiological impact. Here we report the use of the leukocyte coping capacity (LCC) technique to quantify the acute stress of capture and handling in brown bears in Scandinavia. In April and May 2012 and 2013, we collected venous blood samples and recorded a range of physiological variables to evaluate the effects of capture and the added impact of surgical implantation or removal of transmitters and sensors. We studied 24 brown bears, including 19 that had abdominal surgery. We found 1) LCC values following capture were lower in solitary bears than in bears in family groups suggesting capture caused relatively more stress in solitary bears, 2) ability to cope with handling stress was better (greater LCC values) in bears with good body condition, and 3) LCC values did not appear to be influenced by surgery. Although further evaluation of this technique is required, our preliminary results support the use of the LCC technique as a quantitative measure of stress.

Evaluating the Efficacy of a Low-Impact Delivery System for In situ Treatment of Sediments Contaminated with Methylmercury and Other Hydrophobic Chemicals

DTIC Science & Technology

2016-02-01

coefficient LCC Lower Canal Creek MeHg methylmercury MNR monitored natural recovery NIEHS National Institute of Environmental Health Services PAC... Health Command, Naval Facilities Engineering Command Atlantic Division (NAVFAC LANT), U.S. Air Force (USAF), Engineer Research and Development Center...Project ER-200835); (3) a PCB-contaminated tidal creek (Bailey Creek) at Fort Eustis in Virginia (National Institute of Environmental Health Services

Electrochemical energy storage subsystems study, volume 1

NASA Technical Reports Server (NTRS)

Miller, F. Q.; Richardson, P. W.; Graff, C. L.; Jordan, M. V.; Patterson, V. L.

1981-01-01

The effects on life cycle costs (LCC) of major design and performance technology parameters for multi kW LEO and GEO energy storage subsystems using NiCd and NiH2 batteries and fuel cell/electrolysis cell devices were examined. Design, performance and LCC dynamic models are developed based on mission and system/subsystem requirements and existing or derived physical and cost data relationships. The models define baseline designs and costs. The major design and performance parameters are each varied to determine their influence on LCC around the baseline values.

Electrochemical Energy Storage Subsystems Study, Volume 2

NASA Technical Reports Server (NTRS)

Miller, F. Q.; Richardson, P. W.; Graff, C. L.; Jordan, M. V.; Patterson, V. L.

1981-01-01

The effects on life cycle costs (LCC) of major design and performance technology parameters for multi kW LEO and GEO energy storage subsystems using NiCd and NiH2 batteries and fuel cell/electrolysis cell devices were examined. Design, performance and LCC dynamic models are developed based on mission and system/subsystem requirements and existing or derived physical and cost data relationships. The models are exercised to define baseline designs and costs. Then the major design and performance parameters are each varied to determine their influence on LCC around the baseline values.

The mixture of cashew nut shell liquid and castor oil results in an efficient larvicide against Aedes aegypti that does not alter embryo-fetal development, reproductive performance or DNA integrity

PubMed Central

Vani, Juliana Miron; Monreal, Maria Tereza Ferreira Duenhas; Auharek, Sarah Alves; Cunha-Laura, Andréa Luiza; de Arruda, Eduardo José; Lima, Alessandra Ramos; da Silva, Cicera Maria; Antoniolli-Silva, Andréia Conceição Milan Brochado; de Lima, Dênis Pires; Beatriz, Adilson

2018-01-01

Dengue fever, chikungunya fever and Zika virus are epidemics in Brazil that are transmitted by mosquitoes, such as Aedes aegypti or Aedes albopictus. The liquid from shells of cashew nuts is attractive for its important biological and therapeutic activities, which include toxicity to mosquitoes of the genus Aedes. The present study evaluated the effects of a mixture of surfactants from natural cashew nutshell liquid and castor oil (named TaLCC-20) on the mortality of larvae and on the reproductive performance, embryonic and fetal development and genetic stability of Swiss mice. A total of 400 Ae. aegypti larvae (third larval stage) were treated with TaLCC-20 concentrations of 0.05 mg/L, 0.5 mg/L, or 5 mg/L (ppm). Twenty pregnant female mice were also orally administered TaLCC-20 at doses of 5 mg/kg and 50 mg/kg body weight (b.w.), and 10 animals were given only drinking water at 0.1 mL/10 g b.w. (orally). The results of a larvicide test demonstrated that 5 mg/mL TaLCC-20 killed 100% of larvae within three hours, which is comparable to the gold standard indicated by the Ministry of Health. Overall, these results show that TaLCC-20 is an efficient larvicide that does not induce genetic damage. In addition, changes in reproductive performance and embryo-fetal development appear positive, and the formulation is cost effective. Therefore, TaLCC-20 is an important product in the exploration of natural larvicides and can assist in fighting mosquitos as vectors for dengue fever, chikungunya fever and Zika virus, which are emerging/re-emerging and require proper management to ensure minimal harm to the human population. Therefore, TaLCC-20 can be considered a key alternative to commercial products, which are effective yet toxigenic. PMID:29554095

The mixture of cashew nut shell liquid and castor oil results in an efficient larvicide against Aedes aegypti that does not alter embryo-fetal development, reproductive performance or DNA integrity.

PubMed

Vani, Juliana Miron; Monreal, Maria Tereza Ferreira Duenhas; Auharek, Sarah Alves; Cunha-Laura, Andréa Luiza; de Arruda, Eduardo José; Lima, Alessandra Ramos; da Silva, Cicera Maria; Antoniolli-Silva, Andréia Conceição Milan Brochado; de Lima, Dênis Pires; Beatriz, Adilson; Oliveira, Rodrigo Juliano

2018-01-01

Dengue fever, chikungunya fever and Zika virus are epidemics in Brazil that are transmitted by mosquitoes, such as Aedes aegypti or Aedes albopictus. The liquid from shells of cashew nuts is attractive for its important biological and therapeutic activities, which include toxicity to mosquitoes of the genus Aedes. The present study evaluated the effects of a mixture of surfactants from natural cashew nutshell liquid and castor oil (named TaLCC-20) on the mortality of larvae and on the reproductive performance, embryonic and fetal development and genetic stability of Swiss mice. A total of 400 Ae. aegypti larvae (third larval stage) were treated with TaLCC-20 concentrations of 0.05 mg/L, 0.5 mg/L, or 5 mg/L (ppm). Twenty pregnant female mice were also orally administered TaLCC-20 at doses of 5 mg/kg and 50 mg/kg body weight (b.w.), and 10 animals were given only drinking water at 0.1 mL/10 g b.w. (orally). The results of a larvicide test demonstrated that 5 mg/mL TaLCC-20 killed 100% of larvae within three hours, which is comparable to the gold standard indicated by the Ministry of Health. Overall, these results show that TaLCC-20 is an efficient larvicide that does not induce genetic damage. In addition, changes in reproductive performance and embryo-fetal development appear positive, and the formulation is cost effective. Therefore, TaLCC-20 is an important product in the exploration of natural larvicides and can assist in fighting mosquitos as vectors for dengue fever, chikungunya fever and Zika virus, which are emerging/re-emerging and require proper management to ensure minimal harm to the human population. Therefore, TaLCC-20 can be considered a key alternative to commercial products, which are effective yet toxigenic.

'Sweeter Than a Swisher': amount and themes of little cigar and cigarillo content on Twitter.

PubMed

Kostygina, Ganna; Tran, Hy; Shi, Yaru; Kim, Yoonsang; Emery, Sherry

2016-10-01

Despite recent increases in little cigar and cigarillo (LCC) use-particularly among urban youth, African-Americans and Latinos-research on targeted strategies for marketing these products is sparse. Little is known about the amount or content of LCC messages users see or share on social media, a popular communication medium among youth and communities of colour. Keyword rules were used to collect tweets related to LCCs from the Twitter Firehose posted in October 2014 and March-April 2015. Tweets were coded for promotional content, brand references, co-use with marijuana and subculture references (eg, rap/hip-hop, celebrity endorsements) and were classified as commercial and 'organic'/non-commercial using a combination of machine learning methods, keyword algorithms and human coding. Metadata associated with each tweet were used to categorise users as influencers (1000 and more followers) and regular users (under 1000 followers). Keyword filters captured over 4 372 293 LCC tweets. Analyses revealed that 17% of account users posting about LCCs were influencers and 1% of accounts were overtly commercial. Influencers were more likely to mention LCC brands and post promotional messages. Approximately 83% of LCC tweets contained references to marijuana and 29% of tweets were memes. Tweets also contained references to rap/hip-hop lyrics and urban subculture. Twitter is a major information-sharing and marketing platform for LCCs. Co-use of tobacco and marijuana is common and normalised on Twitter. The presence and broad reach of LCC messages on social media warrants urgent need for surveillance and serious attention from public health professionals and policymakers. Future tobacco use prevention initiatives should be adapted to ensure that they are inclusive of LCC use. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/.

Nucleus-Selective Expression of Laccase Genes in the Dikaryotic Strain of Lentinula edodes.

PubMed

Ha, Byeongsuk; Lee, Sieun; Kim, Sinil; Kim, Minseek; Moon, Yoon Jung; Song, Yelin; Ro, Hyeon-Su

2017-12-01

In mating of Lentinula edodes , dikaryotic strains generated from certain monokaryotic strains such as the B2 used in this study tend to show better quality of fruiting bodies regardless of the mated monokaryotic strains. Unlike B2, dikaryotic strains generated from B16 generally show low yields, with deformed or underdeveloped fruiting bodies. This indicates that the two nuclei in the cytoplasm do not contribute equally to the physiology of dikaryotic L. edodes , suggesting an expression bias in the allelic genes of the two nuclei. To understand the role of each nucleus in dikaryotic strains, we investigated single nucleotide polymorphisms (SNPs) in laccase genes of monokaryotic strains to reveal nuclear origin of the expressed mRNAs in dikaryotic strain. We performed reverse transcription PCR (RT-PCR) analysis using total RNAs extracted from dikaryotic strains (A5B2, A18B2, and A2B16) as well as from compatible monokaryotic strains (A5, A18, and B2 for A5B2 and A18B2; A2 and B16 for A2B16). RT-PCR results revealed that Lcc1, Lcc2, Lcc4, Lcc7, and Lcc10 were the mainly expressed laccase genes in the L. edodes genome. To determine the nuclear origin of these laccase genes, the genomic DNA sequences in monokaryotic strains were analyzed, thereby revealing five SNPs in Lcc4 and two in Lcc7. Subsequent sequence analysis of laccase mRNAs expressed in dikaryotic strains revealed that these were almost exclusively expressed from B2-originated nuclei in A5B2 and A18B2 whereas B16 nucleus did not contribute to laccase expression in A2B16 strain. This suggests that B2 nucleus dominates the expression of allelic genes, thereby governing the physiology of dikaryons.

Nucleus-Selective Expression of Laccase Genes in the Dikaryotic Strain of Lentinula edodes

PubMed Central

Ha, Byeongsuk; Lee, Sieun; Kim, Sinil; Kim, Minseek; Moon, Yoon Jung; Song, Yelin

2017-01-01

In mating of Lentinula edodes, dikaryotic strains generated from certain monokaryotic strains such as the B2 used in this study tend to show better quality of fruiting bodies regardless of the mated monokaryotic strains. Unlike B2, dikaryotic strains generated from B16 generally show low yields, with deformed or underdeveloped fruiting bodies. This indicates that the two nuclei in the cytoplasm do not contribute equally to the physiology of dikaryotic L. edodes, suggesting an expression bias in the allelic genes of the two nuclei. To understand the role of each nucleus in dikaryotic strains, we investigated single nucleotide polymorphisms (SNPs) in laccase genes of monokaryotic strains to reveal nuclear origin of the expressed mRNAs in dikaryotic strain. We performed reverse transcription PCR (RT-PCR) analysis using total RNAs extracted from dikaryotic strains (A5B2, A18B2, and A2B16) as well as from compatible monokaryotic strains (A5, A18, and B2 for A5B2 and A18B2; A2 and B16 for A2B16). RT-PCR results revealed that Lcc1, Lcc2, Lcc4, Lcc7, and Lcc10 were the mainly expressed laccase genes in the L. edodes genome. To determine the nuclear origin of these laccase genes, the genomic DNA sequences in monokaryotic strains were analyzed, thereby revealing five SNPs in Lcc4 and two in Lcc7. Subsequent sequence analysis of laccase mRNAs expressed in dikaryotic strains revealed that these were almost exclusively expressed from B2-originated nuclei in A5B2 and A18B2 whereas B16 nucleus did not contribute to laccase expression in A2B16 strain. This suggests that B2 nucleus dominates the expression of allelic genes, thereby governing the physiology of dikaryons. PMID:29371806

Maritime Tactical Command and Control Analysis of Alternatives

DTIC Science & Technology

2016-01-01

JIIM joint, interagency, intergovernmental, and multinational LCC life-cycle cost MANA Map Aware Non-Uniform Automata MDA milestone decision authority...Map Aware Non-Uniform Automata (MANA), a combat and C4I, surveillance, and reconnaissance model developed by the New Zealand Defence Technology

Land-cover change in the Gulf Coastal Plains and Ozarks Landscape Conservation Cooperative, 1973 to 2000

USGS Publications Warehouse

Drummond, Mark A.; Stier, Michael P.; Coffin, Alisa W.

2015-01-01

This report summarizes baseline land-cover change information for four time intervals from between 1973 and 2000 for the Gulf Coastal Plains and Ozarks Landscape Conservation Cooperative (LCC). The study used sample data from the USGS Land Cover Trends dataset to develop estimates of change for 10 land-cover classes in the LCC. The results show that an estimated 17.7 percent of the LCC land cover had a change during the 27-year period. Cyclic forest dynamics—of timber harvest and regrowth—are the most extensive types of land conversion. Agricultural land had an estimated net decline of 3.5 percent as cropland and pasture were urbanized and developed and converted to forest use. Urban and other developed land covers expanded from 2.0 percent of the LCC in 1973 to 3.1 percent in 2000. The report also highlights causes and challenges of land-cover change.

A Novel Lentinula edodes Laccase and Its Comparative Enzymology Suggest Guaiacol-Based Laccase Engineering for Bioremediation

PubMed Central

Wong, Kin-Sing; Cheung, Man-Kit; Au, Chun-Hang; Kwan, Hoi-Shan

2013-01-01

Laccases are versatile biocatalysts for the bioremediation of various xenobiotics, including dyes and polyaromatic hydrocarbons. However, current sources of new enzymes, simple heterologous expression hosts and enzymatic information (such as the appropriateness of common screening substrates on laccase engineering) remain scarce to support efficient engineering of laccase for better “green” applications. To address the issue, this study began with cloning the laccase family of Lentinula edodes. Three laccases perfectio sensu stricto (Lcc4A, Lcc5, and Lcc7) were then expressed from Pichia pastoris, characterized and compared with the previously reported Lcc1A and Lcc1B in terms of kinetics, stability, and degradation of dyes and polyaromatic hydrocarbons. Lcc7 represented a novel laccase, and it exhibited both the highest catalytic efficiency (assayed with 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) [ABTS]) and thermostability. However, its performance on “green” applications surprisingly did not match the activity on the common screening substrates, namely, ABTS and 2,6-dimethoxyphenol. On the other hand, correlation analyses revealed that guaiacol is much better associated with the decolorization of multiple structurally different dyes than are the two common screening substrates. Comparison of the oxidation chemistry of guaiacol and phenolic dyes, such as azo dyes, further showed that they both involve generation of phenoxyl radicals in laccase-catalyzed oxidation. In summary, this study concluded a robust expression platform of L. edodes laccases, novel laccases, and an indicative screening substrate, guaiacol, which are all essential fundamentals for appropriately driving the engineering of laccases towards more efficient “green” applications. PMID:23799101

Corrupt practices negatively influenced food security and live expectancy in developing countries

PubMed Central

Uchendu, Florence Ngozi; Abolarin, Thaddeus Olatunbosun

2015-01-01

Malnutrition is a global public health problem more prevalent in developing countries than in developed countries. Indicators of malnutrition include household food security and life expectancy. Corruption might be one of socio-political problems fuelling malnutrition in developing countries. The aim of this paper is to compare influence of corruption on food security, live expectancy (LE) and population in developed and developing countries. Thirty two least corrupt countries (LCC) and most corrupt countries (MCC) representing developed and developing countries were systematically selected using Corruption Perceptions Index (CPI). Countries’ data on population, food security index (FSI) and LE scores were obtained from Global food security index (GFSI) and Population reference bureau. T-test, Multivariate (Wilks’ Lambda), Pearson product moment analysis were performed to determine relationship between CPI, FSI, LE, and population in LCC and MCC at p<.05. Data were presented in tables, means and percentages. Mean CPI, Population, FSI, and LE in LCC and MCC were 71.5% and 24.2%; 34.8 and 41.7million; 75.0% and 37.4%; and 78.4years and 62.4years. There was a significant difference between CPI, FSI and LE in LCC and MCC (p < 0.05). CPI had a significant positive relationship with FSI and LE in LCC not MCC. There was also a significant relationship between FSI and LE in MCC. Low CPI influenced high FSI and LE in LCC while Low LE was associated with low FSI in MCC. Policies discouraging corrupt practices and promoting good governance should be embraced to eradicate malnutrition in developing countries. PMID:26090058

Northwest Boreal Landscape Conservation Cooperative strategic plan 2015 - 2025

USGS Publications Warehouse

Markon, Carl; Schroff, Eric

2016-01-01

The Northwest Boreal Landscape Conservation Cooperative (NWB LCC) is a voluntary, diverse, self-directed management-science partnership, informing and promoting integrated science, sustainable natural and cultural resource management, and conservation to address impacts of climate change and other stressors within and across ecosystems. The NWB LCC area includes parts of Alaska, Yukon, Northwest Territories, and British Columbia. Our partnership reflects both the broad geographic scope and an extensive array of active and engaged participants including resource management organizations, government representatives, policy makers, Tribes and First Nations, industry leaders, researchers, non-governmental organizations, and research/education institutions. Bringing together diverse partners will help assure the northwest boreal is a functioning, sustainable landscape. We live in an era of profound conservation challenges, including the loss and fragmentation of habitats, genetic isolation, invasive species, and unnatural wildfire. The effects of rapidly changing climate are already evident on the landscape. In these circumstances, it is imperative that natural resource management agencies, science providers, Tribes, First Nations, conservation organizations, and other stakeholders work together to understand the drivers and impacts of landscape change and to determine how best to address those challenges. Further, it is essential that the public and communities receive clear communication about the vision and activities of the NWB LCC. Open public access to NWB LCC activities and products will promote acceptance and support of the science that guides potential changes in management action and conservation strategy. This strategic plan provides a great opportunity for the NWB LCC to share our approach and intentions to the LCC members, collaborators, communities, and the public at large.

Corrupt practices negatively influenced food security and live expectancy in developing countries.

PubMed

Uchendu, Florence Ngozi; Abolarin, Thaddeus Olatunbosun

2015-01-01

Malnutrition is a global public health problem more prevalent in developing countries than in developed countries. Indicators of malnutrition include household food security and life expectancy. Corruption might be one of socio-political problems fuelling malnutrition in developing countries. The aim of this paper is to compare influence of corruption on food security, live expectancy (LE) and population in developed and developing countries. Thirty two least corrupt countries (LCC) and most corrupt countries (MCC) representing developed and developing countries were systematically selected using Corruption Perceptions Index (CPI). Countries' data on population, food security index (FSI) and LE scores were obtained from Global food security index (GFSI) and Population reference bureau. T-test, Multivariate (Wilks' Lambda), Pearson product moment analysis were performed to determine relationship between CPI, FSI, LE, and population in LCC and MCC at p < .05. Data were presented in tables, means and percentages. Mean CPI, Population, FSI, and LE in LCC and MCC were 71.5% and 24.2%; 34.8 and 41.7 million; 75.0% and 37.4%; and 78.4 years and 62.4 years. There was a significant difference between CPI, FSI and LE in LCC and MCC (p < 0.05). CPI had a significant positive relationship with FSI and LE in LCC not MCC. There was also a significant relationship between FSI and LE in MCC. Low CPI influenced high FSI and LE in LCC while Low LE was associated with low FSI in MCC. Policies discouraging corrupt practices and promoting good governance should be embraced to eradicate malnutrition in developing countries.

Lessons Learned for Planning and Estimating Operations Support Requirements

NASA Technical Reports Server (NTRS)

Newhouse, Marilyn

2011-01-01

Operations (phase E) costs are typically small compared to the spacecraft development and test costs. This, combined with the long lead time for realizing operations costs, can lead projects to focus on hardware development schedules and costs, de-emphasizing estimation of operations support requirements during proposal, early design, and replan cost exercises. The Discovery and New Frontiers (D&NF) programs comprise small, cost-capped missions supporting scientific exploration of the solar system. Even moderate yearly underestimates of the operations costs can present significant LCC impacts for deep space missions with long operational durations, and any LCC growth can directly impact the programs ability to fund new missions. The D&NF Program Office at Marshall Space Flight Center recently studied cost overruns for 7 D&NF missions related to phase C/D development of operational capabilities and phase E mission operations. The goal was to identify the underlying causes for the overruns and develop practical mitigations to assist the D&NF projects in identifying potential operations risks and controlling the associated impacts to operations development and execution costs. The study found that the drivers behind these overruns include overly optimistic assumptions regarding the savings resulting from the use of heritage technology, late development of operations requirements, inadequate planning for sustaining engineering and the special requirements of long duration missions (e.g., knowledge retention and hardware/software refresh), and delayed completion of ground system development work. This presentation summarizes the study and the results, providing a set of lessons NASA can use to improve early estimation and validation of operations costs.

Topological Transformation of Defects in Nematic Liquid Crystals

NASA Astrophysics Data System (ADS)

Pagel, Zachary; Atherton, Timothy; Guasto, Jeffrey; Cebe, Peggy

A topological transformation around silica microsphere inclusions in nematic liquid crystal cells (LCC) is experimentally studied. Silica microspheres are coated to induce homeotropic LC anchoring to the spheres. Parallel rub directions of the alignment polymer during LCC construction create a splay wall that traps the microspheres. Application of an out-of-plane electric field then permits a transformation of hedgehog defects, reversing the orientation of the defect around microspheres. The transformation controllably reverses the microsphere's direction of travel during AC electrophoresis due to defect-dependent velocity anisotropy. A similar transformation is studied on chains of microspheres with hedgehog defects, where the defect orientation is reversed on the entire chain. Polarized and confocal microscopies are used to study the defect structures. Results contribute to recent developments in microsphere electrokinetics in nematic LCs, as the transformation adds an additional degree of control in the electrophoretic motion of microspheres and chains of microspheres with dipolar defects. The author thanks NSF Grant DMR-1608126 for funding reseearch and Tufts University for funding travel.

Report on the comparison of the scan strategies employed by the Patrick Air Force Base WSR-74C/McGill radar and the NWS Melbourne WSR-88D radar

NASA Technical Reports Server (NTRS)

Taylor, Gregory; Evans, Randolph; Manobianco, John; Schumann, Robin; Wheeler, Mark; Yersavich, Ann

1994-01-01

The objective of this investigation is to determine whether the current standard WSR-88D radar (NEXRAD) scan strategies permit the use of the Melbourne WSR-88D to perform the essential functions now performed by the Patrick Air Force Base (PAFB) WSR-74C/McGill radar for evaluating shuttle weather flight rules (FR) and launch commit criteria (LCC). To meet this objective, the investigation compared the beam coverage patterns of the WSR-74C/McGill radar located at PAFB and the WSR-88D radar located at the Melbourne National Weather Service (NWS) Office over the area of concern for weather FR and LCC evaluations. The analysis focused on beam coverage within four vertical 74 km radius cylinders (1 to 4 km above ground level (AGL), 4 to 8 km AGL, 8 to 12 km AGL, and 1 to 12 km AGL) centered on Kennedy Space Center (KSC) Launch Complex 39A. The PAFB WSR-74C/McGill radar is approximately 17 km north-northeast of the Melbourne WSR-88D radar. The beam coverage of the WSR-88D using VCP 11 located at the Melbourne NWS Office is comparable (difference in percent of the atmosphere sampled between the two radars is 10 percent or less) within the area of concern to the beam coverage of the WSR-74C/McGill radar located at PAFB. Both radars provide good beam coverage over much of the atmospheric region of concern. In addition, both radars provide poor beam coverage (coverage less than 50 percent) over limited regions near the radars due to the radars' cone of silence and gaps in coverage within the higher elevation scans. Based on scan strategy alone, the WSR-88D radar could be used to perform the essential functions now performed by the PAFB WSR-74C/McGill radar for evaluating shuttle weather FR and LCC. Other radar characteristics may, however, affect the decision as to which radar to use in a given case.

Development of three-dimensional lung multicellular spheroids in air- and liquid-interface culture for the evaluation of anticancer therapeutics.

PubMed

Meenach, Samantha A; Tsoras, Alexandra N; McGarry, Ronald C; Mansour, Heidi M; Hilt, J Zach; Anderson, Kimberly W

2016-04-01

Three-dimensional (3D) lung multicellular spheroids (MCS) in liquid-covered culture (LCC) and air-interface culture (AIC) conditions have both been developed for the evaluation of aerosol anticancer therapeutics in solution and aerosols, respectively. The MCS were formed by seeding lung cancer cells on top of collagen where they formed spheroids due to the prevalence of cell-to-cell interactions. LCC MCS were exposed to paclitaxel (PTX) in media whereas AIC MCS were exposed to dry powder PEGylated phospholipid aerosol microparticles containing paclitaxel. The difference in viability for 2D versus 3D culture for both LCC and AIC was evaluated along with the effects of the particles on lung epithelium via transepithelial electrical resistance (TEER) measurements. For LCC and AIC conditions, the 3D spheroids were more resistant to treatment with higher IC50 values for A549 and H358 cell lines. TEER results initially indicated a decrease in resistance upon drug or particle exposure, however, these values increased over the course of several days indicating the ability of the cells to recover. Overall, these studies offer a comprehensive in vitro evaluation of aerosol particles used in the treatment of lung cancer while introducing a new method for culturing lung cancer MCS in both LCC and AIC conditions.

The severity of iron chlorosis in sensitive plants is related to soil phosphorus levels.

PubMed

Sánchez-Rodríguez, Antonio Rafael; del Campillo, María Carmen; Torrent, José

2014-10-01

Iron (Fe) deficiency chlorosis, a major nutritional problem in plants growing on calcareous soils, is related to the content and reactivity of soil iron oxides and carbonates. The effects of other soil components, however, need elucidation. In this paper we tested the hypothesis that application of high doses of phosphorus (P) to the soil can aggravate Fe chlorosis. Lupin and sorghum were grown on 24 calcareous soils. Leaf chlorophyll concentration (LCC) in lupin decreased with increasing available P/available Fe ratio in the native soil but LCC in sorghum was unaffected by that ratio. Application of P to the soil resulted in significant reduction of LCC and dry weight in lupin. In sorghum, LCC and dry weight were positively affected by P fertilisation for soils poor in available P whereas the opposite effect was generally observed for the P-rich soils. In another experiment where olive plants were pot-grown on two soils during the 2009–2011 period, P fertilisation affected LCC negatively only in 2009 and 2011 and in the soil that was poorer in iron oxides. Application of fertiliser P to Fe chlorosis-inducing soils is likely to aggravate this deficiency. However, this effect depends on the plant and the Fe and P statuses of the soil. © 2014 Society of Chemical Industry.

An Estimate of the Vertical Variability of Temperature at KSC Launch Complex 39-B

NASA Technical Reports Server (NTRS)

Brenton, James

2017-01-01

The purpose of this analysis is to determine the vertical variability of the air temperature below 500 feet at Launch Complex (LC) 39-B at Kennedy Space Center (KSC). This analysis utilizes data from the LC39-B Lightning Protection System (LPS) Towers and the 500 foot Tower 313. The results of this analysis will be used to help evaluate the ambient air temperature Launch Commit Criteria (LCC) for the Exploration Mission 1 launch.

ASCANS Saturn V & LCC Tour

NASA Image and Video Library

2014-03-03

CAPE CANAVERAL, Fla. – NASA astronaut candidates Anne McClain, from left, Andrew Morgan, Nicole Mann, Victor Glover, Christina Hammock and Josh Cassada observe the Apollo 14 command module which carried astronauts Alan Shepard, Stu Roosa and Edgar Mitchell on their lunar landing mission in 1971.The astronauts toured the Apollo Saturn V Center at Kennedy Space Center in Florida during a daylong set of briefings and tours of different facilities at NASA's primary launch center. The astronaut class of 2013 was selected by NASA after an extensive year-and-a-half search. The new group will help the agency push the boundaries of exploration and travel to new destinations in the solar system. To learn more about the astronaut class of 2013, visit: http://www.nasa.gov/astronauts/2013astroclass.html Photo credit: NASA/Kim Shiflett

Lightning Launch Commit Criteria for America's Space Program

NASA Technical Reports Server (NTRS)

Roeder, W. P.; Sardonia, J. E.; Jacobs, S. C.; Hinson, M. S.; Harms, D. E.; Madura, J. T.; DeSordi, S. P.

1999-01-01

The danger of natural and triggered lightning significantly impacts space launch operations supported by the USAF. The lightning Launch Commit Criteria (LCC) are used by the USAF to avoid these lightning threats to space launches. This paper presents a brief overview of the LCC.

Shrinking Sea Ice, Thawing Permafrost, Bigger Storms, and Extremely Limited Data - Addressing Information Needs of Stakeholders in Western Alaska Through Participatory Decisions and Collaborative Science.

NASA Astrophysics Data System (ADS)

Murphy, K. A.; Reynolds, J.

2015-12-01

Communities, Tribes, and decision makers in coastal western Alaska are being impacted by declining sea ice, sea level rise, changing storm patterns and intensities, and increased rates of coastal erosion. Relative to their counterparts in the contiguous USA, their ability to plan for and respond to these changes is constrained by the region's generally meager or non-existent information base. Further, the information needs and logistic challenges are of a scale that perhaps can be addressed only through strong, strategic collaboration. Landscape Conservation Cooperatives (LCCs) are fundamentally about applied science and collaboration, especially collaborative decision making. The Western Alaska LCC has established a process of participatory decision making that brings together researchers, agency managers, local experts from Tribes and field specialists to identify and prioritize shared information needs; develop a course of action to address them by using the LCC's limited resources to catalyze engagement, overcome barriers to progress, and build momentum; then ensure products are delivered in a manner that meets decision makers' needs. We briefly review the LCC's activities & outcomes from the stages of (i) collaborative needs assessment (joint with the Alaska Climate Science Center and the Alaska Ocean Observing System), (ii) strategic science activities, and (iii) product refinement and delivery. We discuss lessons learned, in the context of our recent program focused on 'Changes in Coastal Storms and Their Impacts' and current collaborative efforts focused on delivery of Coastal Resiliency planning tools and results from applied science projects. Emphasis is given to the various key interactions between scientists and decision makers / managers that have been promoted by this process to ensure alignment of final products to decision maker needs.

Need for Cost Optimization of Space Life Support Systems

NASA Technical Reports Server (NTRS)

Jones, Harry W.; Anderson, Grant

2017-01-01

As the nation plans manned missions that go far beyond Earth orbit to Mars, there is an urgent need for a robust, disciplined systems engineering methodology that can identify an optimized Environmental Control and Life Support (ECLSS) architecture for long duration deep space missions. But unlike the previously used Equivalent System Mass (ESM), the method must be inclusive of all driving parameters and emphasize the economic analysis of life support system design. The key parameter for this analysis is Life Cycle Cost (LCC). LCC takes into account the cost for development and qualification of the system, launch costs, operational costs, maintenance costs and all other relevant and associated costs. Additionally, an effective methodology must consider system technical performance, safety, reliability, maintainability, crew time, and other factors that could affect the overall merit of the life support system.

Micro-Spectroscopic Imaging of Lignin-Carbohydrate Complexes in Plant Cell Walls and Their Migration During Biomass Pretreatment

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zeng, Yining; Zhao, Shuai; Wei, Hui

2015-04-27

In lignocellulosic biomass, lignin is the second most abundant biopolymer. In plant cell walls, lignin is associated with polysaccharides to form lignin-carbohydrate complexes (LCC). LCC have been considered to be a major factor that negatively affects the process of deconstructing biomass to simple sugars by cellulosic enzymes. Here, we report a micro-spectroscopic approach that combines fluorescence lifetime imaging microscopy and Stimulated Raman Scattering microscopy to probe in situ lignin concentration and conformation at each cell wall layer. This technique does not require extensive sample preparation or any external labels. Using poplar as a feedstock, for example, we observe variation ofmore » LCC in untreated tracheid poplar cell walls. The redistribution of LCC at tracheid poplar cell wall layers is also investigated when the chemical linkages between lignin and hemicellulose are cleaved during pretreatment. Our study would provide new insights into further improvement of the biomass pretreatment process.« less

Marketing little cigars and cigarillos: advertising, price, and associations with neighborhood demographics.

PubMed

Cantrell, Jennifer; Kreslake, Jennifer M; Ganz, Ollie; Pearson, Jennifer L; Vallone, Donna; Anesetti-Rothermel, Andrew; Xiao, Haijun; Kirchner, Thomas R

2013-10-01

We have documented little cigar and cigarillo (LCC) availability, advertising, and price in the point-of-sale environment and examined associations with neighborhood demographics. We used a multimodal real-time surveillance system to survey LCCs in 750 licensed tobacco retail outlets that sold tobacco products in Washington, DC. Using multivariate models, we examined the odds of LCC availability, the number of storefront exterior advertisements, and the price per cigarillo for Black & Mild packs in relation to neighborhood demographics. The odds of LCC availability and price per cigarillo decreased significantly in nearly a dose-response manner with each quartile increase in proportion of African Americans. Prices were also lower in some young adult neighborhoods. Having a higher proportion of African American and young adult residents was associated with more exterior LCC advertising. Higher availability of LCCs in African American communities and lower prices and greater outdoor advertising in minority and young adult neighborhoods may establish environmental triggers to smoke among groups susceptible to initiation, addiction, and long-term negative health consequences.

A FORMATION SCENARIO OF YOUNG STELLAR GROUPS IN THE REGION OF THE SCORPIO CENTAURUS OB ASSOCIATION

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ortega, V. G.; Jilinski, E.; De la Reza, R.

2009-04-15

The main objective of this work is to investigate the role played by Lower Centaurus Crux (LCC) and Upper Centaurus Lupus (UCL), both subcomponents of the Scorpio Centaurus OB association (Sco-Cen), in the formation of the groups {beta} Pictoris, TW Hydrae, and the {eta} Chamaeleontis cluster. The dynamical evolution of all the stellar groups involved and of the bubbles and shells blown by LCC and UCL are calculated, and followed from the past to the present. This leads to a formation scenario in which (1) the groups {beta} Pictoris, TW Hydrae were formed in the wake of the shells createdmore » by LCC and UCL, (2) the young cluster {eta} Chamaeleontis was born as a consequence of the collision of the shells of LCC and UCL, and (3) the formation of Upper Scorpius (US), the other main subcomponent of the Sco-Cen association, may have been started by the same process that created {eta} Chamaeleontis.« less

Orpheovirus IHUMI-LCC2: A New Virus among the Giant Viruses

PubMed Central

Andreani, Julien; Khalil, Jacques Y. B.; Baptiste, Emeline; Hasni, Issam; Michelle, Caroline; Raoult, Didier; Levasseur, Anthony; La Scola, Bernard

2018-01-01

Giant viruses continue to invade the world of virology, in gigantic genome sizes and various particles shapes. Strains discoveries and metagenomic studies make it possible to reveal the complexity of these microorganisms, their origins, ecosystems and putative roles. We isolated from a rat stool sample a new giant virus “Orpheovirus IHUMI-LCC2,” using Vermamoeba vermiformis as host cell. In this paper, we describe the main genomic features and replicative cycle of Orpheovirus IHUMI-LCC2. It possesses a circular genome exceeding 1.4 Megabases with 25% G+C content and ovoidal-shaped particles ranging from 900 to 1300 nm. Particles are closed by at least one thick membrane in a single ostiole-like shape in their apex. Phylogenetic analysis and the reciprocal best hit for Orpheovirus show a connection to the proposed Pithoviridae family. However, some genomic characteristics bear witness to a completely divergent evolution for Orpheovirus IHUMI-LCC2 when compared to Cedratviruses or Pithoviruses. PMID:29403444

Biophysical effects on temperature and precipitation due to land cover change

NASA Astrophysics Data System (ADS)

Perugini, Lucia; Caporaso, Luca; Marconi, Sergio; Cescatti, Alessandro; Quesada, Benjamin; de Noblet-Ducoudré, Nathalie; House, Johanna I.; Arneth, Almut

2017-05-01

Anthropogenic land cover changes (LCC) affect regional and global climate through biophysical variations of the surface energy budget mediated by albedo, evapotranspiration, and roughness. This change in surface energy budget may exacerbate or counteract biogeochemical greenhouse gas effects of LCC, with a large body of emerging assessments being produced, sometimes apparently contradictory. We reviewed the existing scientific literature with the objective to provide an overview of the state-of-the-knowledge of the biophysical LCC climate effects, in support of the assessment of mitigation/adaptation land policies. Out of the published studies that were analyzed, 28 papers fulfilled the eligibility criteria, providing surface air temperature and/or precipitation change with respect to LCC regionally and/or globally. We provide a synthesis of the signal, magnitude and uncertainty of temperature and precipitation changes in response to LCC biophysical effects by climate region (boreal/temperate/tropical) and by key land cover transitions. Model results indicate that a modification of biophysical processes at the land surface has a strong regional climate effect, and non-negligible global impact on temperature. Simulations experiments of large-scale (i.e. complete) regional deforestation lead to a mean reduction in precipitation in all regions, while air surface temperature increases in the tropics and decreases in boreal regions. The net global climate effects of regional deforestation are less certain. There is an overall consensus in the model experiments that the average global biophysical climate response to complete global deforestation is atmospheric cooling and drying. Observed estimates of temperature change following deforestation indicate a smaller effect than model-based regional estimates in boreal regions, comparable results in the tropics, and contrasting results in temperate regions. Regional/local biophysical effects following LCC are important for local climate, water cycle, ecosystems, their productivity and biodiversity, and thus important to consider in the formulation of adaptation policy. However before considering the inclusion of biophysical climate effects of LCC under the UNFCCC, science has to provide robust tools and methods for estimation of both country and global level effects.

Lithium Batteries and Supercapacitors Capable of Operating at Low Temperatures for Planetary Exploration

NASA Technical Reports Server (NTRS)

Smart, M. C.; Ratnakumar, B. V.; West, W. C.; Brandon, E. J.

2012-01-01

Demonstrated improved performance with wide operating temperature electrolytes containing ester co - solvents (i.e., methyl propionate and ethyl butyrate) in a number of prototype cells: center dot Successfully scaled up low temperature technology to 12 Ah size prismatic Li - ion cells (Quallion, LCC), and demonstrated good performance down to - 60 o C. center dot Demonstrated wide operating temperature range performance ( - 60 o to +60 o C) in A123 Systems LiFePO 4 - based lithium - ion cells containing methyl butyrate - based low temperature electrolytes. These systems were also demonstrated to have excellent cycle life performance at ambient temperatures, as well as the ability to be cycled up to high temperatures.

Cost analysis of Navy acquisition alternatives for the NAVSTAR Global Positioning System

NASA Astrophysics Data System (ADS)

Darcy, T. F.; Smith, G. P.

1982-12-01

This research analyzes the life cycle cost (LCC) of the Navy's current and two hypothetical procurement alternatives for NAVSTAR Global Positioning System (GPS) user equipment. Costs are derived by the ARINC Research Corporation ACBEN cost estimating system. Data presentation is in a comparative format describing individual alternative LCC and differential costs between alternatives. Sensitivity analysis explores the impact receiver-processor unit (RPU) first unit production cost has on individual alternative LCC, as well as cost differentials between each alternative. Several benefits are discussed that might provide sufficient cost savings and/or system effectiveness improvements to warrant a procurement strategy other than the existing proposal.

Development and Testing of Functionally Operative and Visually Appealing Remote Firing Room Displays and Applications

NASA Technical Reports Server (NTRS)

Quaranto, Kristy

2014-01-01

This internship provided an opportunity for an intern to work with NASA's Ground Support Equipment (GSE) for the Spaceport Command and Control System (SCCS) at Kennedy Space Center as a remote display developer, under NASA technical mentor Kurt Leucht. The main focus was on creating remote displays and applications for the hypergolic and high pressure helium subsystem team to help control the filling of the respective tanks. As a remote display and application developer for the GSE hypergolic and high pressure helium subsystem team the intern was responsible for creating and testing graphical remote displays and applications to be used in the Launch Control Center (LCC) on the Firing Room's computers. To become more familiar with the subsystem, the individual attended multiple project meetings and acquired their specific requirements regarding what needed to be included in the software. After receiving the requirements for the displays, the next step was to create displays that had both visual appeal and logical order using the Display Editor, on the Virtual Machine (VM). In doing so, all Compact Unique Identifiers (CUI), which are associated with specific components within the subsystem, were need to be included in each respective display for the system to run properly. Then, once the display was created it was to be tested to ensure that the display runs as intended by using the Test Driver, also found on the VM. This Test Driver is a specific application that checks to make sure all the CUIs in the display are running properly and returning the correct form of information. After creating and locally testing the display it needed to go through further testing and evaluation before deemed suitable for actual use. For the remote applications the intern was responsible for creating a project that focused on channelizing each component included in each display. The core of the application code was created by setting up spreadsheets and having an auto test generator, generate the complete code structure. This application code was then loaded and ran on a testing environment set to ensure the code runs as anticipated. By the end of the semester-long experience at NASA's Kennedy Space Center, the individual should have gained great knowledge and experience in various areas of both display and application development and testing. They were able to demonstrate this new knowledge obtained by creating multiple successful remote displays that will one day be used by the hypergolic and high pressure helium subsystem team in the LCC's firing rooms to service the new Orion spacecraft. The completed display channelization application will be used to receive verification from NASA quality engineers.

The Life Cycle Cost (LCC) of Life Support Recycling and Resupply

NASA Technical Reports Server (NTRS)

Jones, Harry W.

2015-01-01

Brief human space missions supply all the crew's water and oxygen from Earth. The multiyear International Space Station (ISS) program instead uses physicochemical life support systems to recycle water and oxygen. This paper compares the Life Cycle Cost (LCC) of recycling to the LCC of resupply for potential future long duration human space missions. Recycling systems have high initial development costs but relatively low durationdependent support costs. This means that recycling is more cost effective for longer missions. Resupplying all the water and oxygen requires little initial development cost but has a much higher launch mass and launch cost. The cost of resupply increases as the mission duration increases. Resupply is therefore more cost effective than recycling for shorter missions. A recycling system pays for itself when the resupply LCC grows greater over time than the recycling LCC. The time when this occurs is called the recycling breakeven date. Recycling will cost very much less than resupply for long duration missions within the Earth-Moon system, such as a future space station or Moon base. But recycling would cost about the same as resupply for long duration deep space missions, such as a Mars trip. Because it is not possible to provide emergency supplies or quick return options on the way to Mars, more expensive redundant recycling systems will be needed.

Developmental Mathematics and the Lansing Community College Math Lab.

ERIC Educational Resources Information Center

Rotman, Jack W.

Based on an extensive literature search, this paper reviews recent research and theoretical studies and discusses their applicability to Lansing Community College's (LCC's) Mathematics Laboratory. After noting the steps taken in data collection, part I describes LCC and its Math Lab, which offers developmental courses in a self-paced, mastery…

Effects of land cover change on the tropical circulation in a GCM

NASA Astrophysics Data System (ADS)

Jonko, Alexandra Karolina; Hense, Andreas; Feddema, Johannes Jan

2010-09-01

Multivariate statistics are used to investigate sensitivity of the tropical atmospheric circulation to scenario-based global land cover change (LCC), with the largest changes occurring in the tropics. Three simulations performed with the fully coupled Parallel Climate Model (PCM) are compared: (1) a present day control run; (2) a simulation with present day land cover and Intergovernmental Panel on Climate Change (IPCC) Special Report on Emission Scenarios (SRES) A2 greenhouse gas (GHG) projections; and (3) a simulation with SRES A2 land cover and GHG projections. Dimensionality of PCM data is reduced by projection onto a priori specified eigenvectors, consisting of Rossby and Kelvin waves produced by a linearized, reduced gravity model of the tropical circulation. A Hotelling T 2 test is performed on projection amplitudes. Effects of LCC evaluated by this method are limited to diabatic heating. A statistically significant and recurrent signal is detected for 33% of all tests performed for various combinations of parameters. Taking into account uncertainties and limitations of the present methodology, this signal can be interpreted as a Rossby wave response to prescribed LCC. The Rossby waves are shallow, large-scale motions, trapped at the equator and most pronounced in boreal summer. Differences in mass and flow fields indicate a shift of the tropical Walker circulation patterns with an anomalous subsidence over tropical South America.

The Most Natural Tobacco Used: A Qualitative Investigation of Young Adult Smokers' Risk Perceptions of Flavored Little Cigars and Cigarillos.

PubMed

Sterling, Kymberle L; Fryer, Craig S; Fagan, Pebbles

2016-05-01

Flavored little cigar and cigarillo (LCC) smoking prevalence rate is increasing among young adults; little is known about their comprehension of its risks. To inform tobacco control regulatory policy and prevention methods, we explored young adult smokers' risk perceptions of flavored LCC products and its use. Purposive samples (n = 90) of African American, Hispanic, and white young adults who self-identified as dual (smoked ≥ 1 LCC and cigarette in past 30 days) and cigarette-only (≥1 cigarette in past 30 days) smokers participated in 12 audiotaped focus groups and a semi-structured interview conducted in the Southeastern United States. Participants discussed their experiences smoking flavored LCCs and perceived health risks of smoking flavored LCCs. A brief survey was administered to characterize participants. The participants had a mean age of 25.1 years (SD = 4.5), were majority male (53.1%), and were 60.0% African American, 29.5% white, and 17.5% Hispanic. Along with health risks and addiction, three major themes emerged as underlying contributors of risk perceptions: affect, participants' smoking practices (amount smoked and inhalation), and beliefs about the components of LCCs (including flavoring and filters). Participants' reported intention to smoke flavored LCCs with its tobacco or as blunts (filled with marijuana) also influenced perceptions. Flavored LCCs were viewed along a continuum of risks compared to cigarettes and blunt smoking. Our study revealed dimensions that were important for the formation of risk perceptions about flavored LCCs. A multidimensional conceptual model and a measure of risk perceptions that is inclusive of these dimensions should be developed and examined for LCC use patterns. © The Author 2015. Published by Oxford University Press on behalf of the Society for Research on Nicotine and Tobacco. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Influence of infrasound exposure on the whole L-type calcium currents in rat ventricular myocytes.

PubMed

Pei, Zhaohui; Zhuang, Zhiqiang; Xiao, Pingxi; Chen, Jingzao; Sang, Hanfei; Ren, Jun; Wu, Zhenbiao; Yan, Guangmei

2009-06-01

This study was designed to examine the effect of infrasound exposure (5 Hz at 130 dB) on whole-cell L-type Ca2+ currents (WLCC) in rat ventricular myocytes and the underlying mechanism(s) involved. Thirty-two adult Sprague-Dawley rats were randomly assigned to infrasound exposure and control groups. [Ca2+](i), WLCC, mRNA expression of the a(1c) subunit of L-type Ca2+ channels (LCC), and SERCA2 protein were examined on day 1, 7, and 14 after initiation of infrasound exposure. Fluo-3/AM fluorescence and the laser scanning confocal microscope techniques were used to measure [Ca2+](i) in freshly isolated ventricular myocytes. The Ca2+ fluorescence intensity (FI), denoting [Ca2+](i) in cardiomyocytes, was significantly elevated in a time-dependent manner in the exposure groups. There was a significant increase in WLCC in the 1-day group and a further significant increase in the 7- and 14-day groups. LCC mRNA expression measured by RT-PCR revealed a significant rise in the 1-day group and a significant additional rise in the 7- and 14-day groups compared with control group. SERCA2 expression was significantly upregulated in the 1-day group followed by an overt decrease in the 7- and 14-day groups. Prolonged exposure of infrasound altered WLCC in rat cardiomyocytes by shifting the steady-state inactivation curves to the right (more depolarized direction) without altering the slope and biophysical properties of I (Ca,L). Taken together, our data suggest that changes in [Ca2+](I) levels as well as expression of LCC and SERCA2 may contribute to the infrasound exposure-elicited cardiac response.

Marketing Little Cigars and Cigarillos: Advertising, Price, and Associations With Neighborhood Demographics

PubMed Central

Kreslake, Jennifer M.; Ganz, Ollie; Pearson, Jennifer L.; Vallone, Donna; Anesetti-Rothermel, Andrew; Xiao, Haijun; Kirchner, Thomas R.

2013-01-01

Objectives. We have documented little cigar and cigarillo (LCC) availability, advertising, and price in the point-of-sale environment and examined associations with neighborhood demographics. Methods. We used a multimodal real-time surveillance system to survey LCCs in 750 licensed tobacco retail outlets that sold tobacco products in Washington, DC. Using multivariate models, we examined the odds of LCC availability, the number of storefront exterior advertisements, and the price per cigarillo for Black & Mild packs in relation to neighborhood demographics. Results. The odds of LCC availability and price per cigarillo decreased significantly in nearly a dose-response manner with each quartile increase in proportion of African Americans. Prices were also lower in some young adult neighborhoods. Having a higher proportion of African American and young adult residents was associated with more exterior LCC advertising. Conclusions. Higher availability of LCCs in African American communities and lower prices and greater outdoor advertising in minority and young adult neighborhoods may establish environmental triggers to smoke among groups susceptible to initiation, addiction, and long-term negative health consequences. PMID:23948008

User and Content Characteristics of Public Tweets Referencing Little Cigars.

PubMed

Step, Mary M; Bracken, Cheryl C; Trapl, Erika S; Flocke, Susan A

2016-01-01

Compared to cigarettes, little cigars and cigarillos (LCC) are minimally regulated, affordable, and widely available to young people. Because Twitter is a preferred mode of communication among younger people, product portrayals may be useful for informing both interventions and public health or tobacco policy. A mixed-methods study was implemented to analyze the content of public tweets (N = 288) and profile photos sampled from a search of 2 LCC brands (Black & Mild and Swisher Sweets). Metadata and manifest attributes of profile photo demographic features and tweet message features were coded and analyzed. Thematic analysis of the tweets was conducted using an iterative immersion/ crystallization method. Tweeters were most often boys or men (63%) and appeared young (76%). Prevalent content themes included expressing affiliation for the LCC product and reporting smoking activity. Although men and women tweeted affiliation for LCC products and reported smoking activity in similar numbers, women were significantly less likely to tweet about blunting than men. Twitter provides a potentially potent source of nuanced information about how young people are using little cigars. These observed characteristics may be useful to inform counter-messaging strategies and interventions.

Remote Software Application and Display Development

NASA Technical Reports Server (NTRS)

Sanders, Brandon T.

2014-01-01

The era of the shuttle program has come to an end, but only to give rise to newer and more exciting projects. Now is the time of the Orion spacecraft, a work of art designed to exceed all previous endeavors of man. NASA is exiting the time of exploration and is entering a new period, a period of pioneering. With this new mission, many of NASAs organizations must undergo a great deal of change and development to support the Orion missions. The Spaceport Command and Control System (SCCS) is the new system that will provide NASA the ability to launch rockets into orbit and thus control Orion and other spacecraft as the goal of populating Mars becomes ever increasingly tangible. Since the previous control system, Launch Processing System (LPS), was primarily designed to launch the shuttles, SCCS was needed as Kennedy Space Center (KSC) reorganized to a multiuser spaceport for commercial flights, providing a more versatile control over rockets. Within SCCS, is the Launch Control System (LCS), which is the remote software behind the command and monitoring of flight and ground system hardware. This internship at KSC has involved two main components in LCS, including Remote Software Application and Display development. The display environment provides a graphical user interface for an operator to view and see if any cautions are raised, while the remote applications are the backbone that communicate with hardware, and then relay the data back to the displays. These elements go hand in hand as they provide monitoring and control over hardware and software alike from the safety of the Launch Control Center. The remote software applications are written in Application Control Language (ACL), which must undergo unit testing to ensure data integrity. This paper describes both the implementation and writing of unit tests in ACL code for remote software applications, as well as the building of remote displays to be used in the Launch Control Center (LCC).

KSC-2009-3688

NASA Image and Video Library

2009-06-11

CAPE CANAVERAL, Fla. – In the NASA News Center TV Studio at NASA's Kennedy Space Center in Florida, on view is a 1/12 model of the vehicle stabilization system that will be installed on Launch Pad 39B to hold the Ares I-X rocket for its flight test. Looking at the model are (from left) Roger Lenard, consultant with Lee & Associates, LCC; Jon Cowart, NASA's Ares I-X deputy mission manager; and Eric Mellberg, Ares I-X Vehicle Stabilization Design lead with United Space Alliance Ares I-X is the test vehicle for the Ares I, which is part of the Constellation Program to return men to the moon and beyond. Ares I-X is targeted for launch in August 2009. Photo credit: NASA/Kim Shiflett

Identification of the Mechanisms Underlying Antiestrogen Resistance: Breast Cancer Research Partnership Between FIU-UM Braman Family Breast Cancer Institute

DTIC Science & Technology

2011-06-01

sulforaphane contributes to tam sensitivity in tam resistant LCC2 cells by increasing expression of p27. In: Proceedings of the Annual Meeting of...Slingerland, J., and Roy, D. (2010) Modulation of thioredoxin reductase by sulforaphane may restore tamoxifen sensitivity in resistant LCC2 cells

Digital Avionics Information System (DAIS): Training Requirements Analysis Model (TRAMOD).

ERIC Educational Resources Information Center

Czuchry, Andrew J.; And Others

The training requirements analysis model (TRAMOD) described in this report represents an important portion of the larger effort called the Digital Avionics Information System (DAIS) Life Cycle Cost (LCC) Study. TRAMOD is the second of three models that comprise an LCC impact modeling system for use in the early stages of system development. As…

Digital Avionics Information System (DAIS): Mid-1980's Maintenance Task Analysis. Final Report.

ERIC Educational Resources Information Center

Czuchry, Andrew J.; And Others

The fundamental objective of the Digital Avionics Information System (DAIS) Life Cycle Cost (LCC) Study is to provide the Air Force with an enhanced in-house capability to incorporate LCC considerations during all stages of the system acquisition process. The purpose of this report is to describe the technical approach, results, and conclusions…

Holistic Evaluation of Decentralized Water Reuse: Life Cycle Assessment and Cost Analysis of Membrane Bioreactor Systems in Water Reuse Implementation

EPA Science Inventory

Understand environmental and cost impacts of transitional decentralized MBR systems with sewer mining Assess aerobic MBRs (AeMBR) and anaerobic MBRs (AnMBR) Use LCA and life cycle cost (LCC) analysis to quantify impacts Investigate LCA and LCC performance of MBRs under various re...

Tensile and Laterally Confined Compression Properties of Various 3-Dimensional (3-D) Woven Composites

DTIC Science & Technology

2014-11-01

such as orthogonal (Z- fiber) weave, layer-to-layer, and angle interlock. Figure 1 provides an example of 2 different types of 3-D woven structures...o.~os~~~o. t Deflection (in) (c) 90° Orientation 18 6.4 LCC Test Specimen Failure Analysis LCC posttest failure analysis was conducted

76 FR 28025 - East Maui Pumped Storage Water Supply LCC; Notice of Preliminary Permit Application Accepted for...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-13

... Storage Water Supply LCC; Notice of Preliminary Permit Application Accepted for Filing and Soliciting... Act (FPA), proposing to study the feasibility of the East Maui Pumped Storage Water Supply Project to.... Bart M. O'Keeffe, East Maui Pumped Storage Water Supply LLC; P.O. Box 1916; Discovery Bay, CA 94505...

Structural Insights into 2,2′-Azino-Bis(3-Ethylbenzothiazoline-6-Sulfonic Acid) (ABTS)-Mediated Degradation of Reactive Blue 21 by Engineered Cyathus bulleri Laccase and Characterization of Degradation Products

PubMed Central

Kenzom, T.; Srivastava, P.

2014-01-01

Advanced oxidation processes are currently used for the treatment of different reactive dyes which involve use of toxic catalysts. Peroxidases are reported to be effective on such dyes and require hydrogen peroxide and/or metal ions. Cyathus bulleri laccase, expressed in Pichia pastoris, catalyzes efficient degradation (78 to 85%) of reactive azo dyes (reactive black 5, reactive orange 16, and reactive red 198) in the presence of synthetic mediator ABTS [2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)]. This laccase was engineered to degrade effectively reactive blue 21 (RB21), a phthalocyanine dye reported to be decolorized only by peroxidases. The 816-bp segment (toward the C terminus) of the lcc gene was subjected to random mutagenesis and enzyme variants (Lcc35, Lcc61, and Lcc62) were selected based on increased ABTS oxidizing ability. Around 78 to 95% decolorization of RB21 was observed with the ABTS-supplemented Lcc variants in 30 min. Analysis of the degradation products by mass spectrometry indicated the formation of several low-molecular-weight compounds. Mapping the mutations on the modeled structure implicated residues both near and far from the T1 Cu site that affected the catalytic efficiency of the mutant enzymes on ABTS and, in turn, the rate of oxidation of RB21. Several inactive clones were also mapped. The importance of geometry as well as electronic changes on the reactivity of laccases was indicated. PMID:25261507

Structural insights into 2,2'-azino-Bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS)-mediated degradation of reactive blue 21 by engineered Cyathus bulleri Laccase and characterization of degradation products.

PubMed

Kenzom, T; Srivastava, P; Mishra, S

2014-12-01

Advanced oxidation processes are currently used for the treatment of different reactive dyes which involve use of toxic catalysts. Peroxidases are reported to be effective on such dyes and require hydrogen peroxide and/or metal ions. Cyathus bulleri laccase, expressed in Pichia pastoris, catalyzes efficient degradation (78 to 85%) of reactive azo dyes (reactive black 5, reactive orange 16, and reactive red 198) in the presence of synthetic mediator ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)]. This laccase was engineered to degrade effectively reactive blue 21 (RB21), a phthalocyanine dye reported to be decolorized only by peroxidases. The 816-bp segment (toward the C terminus) of the lcc gene was subjected to random mutagenesis and enzyme variants (Lcc35, Lcc61, and Lcc62) were selected based on increased ABTS oxidizing ability. Around 78 to 95% decolorization of RB21 was observed with the ABTS-supplemented Lcc variants in 30 min. Analysis of the degradation products by mass spectrometry indicated the formation of several low-molecular-weight compounds. Mapping the mutations on the modeled structure implicated residues both near and far from the T1 Cu site that affected the catalytic efficiency of the mutant enzymes on ABTS and, in turn, the rate of oxidation of RB21. Several inactive clones were also mapped. The importance of geometry as well as electronic changes on the reactivity of laccases was indicated. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

A new technique for the measurement of surface shear stress vectors using liquid crystal coatings

NASA Technical Reports Server (NTRS)

Reda, Daniel C.; Muratore, J. J., Jr.

1994-01-01

Research has recently shown that liquid crystal coating (LCC) color-change response to shear depends on both shear stress magnitude and direction. Additional research was thus conducted to extend the LCC method from a flow-visualization tool to a surface shear stress vector measurement technique. A shear-sensitive LCC was applied to a planar test surface and illuminated by white light from the normal direction. A fiber optic probe was used to capture light scattered by the LCC from a point on the centerline of a turbulent, tangential-jet flow. Both the relative shear stress magnitude and the relative in-plane view angle between the sensor and the centerline shear vector were systematically varied. A spectrophotometer was used to obtain scattered-light spectra which were used to quantify the LCC color (dominant wavelength) as a function of shear stress magnitude and direction. At any fixed shear stress magnitude, the minimum dominant wavelength was measured when the shear vector was aligned with and directed away from the observer; changes in the relative in-plane view angle to either side of this vector/observer aligned position resulted in symmetric Gaussian increases in measured dominant wavelength. Based on these results, a vector measurement methodology, involving multiple oblique-view observations of the test surface, was formulated. Under present test conditions, the measurement resolution of this technique was found to be +/- 1 deg for vector orientations and +/- 5% for vector magnitudes. An approach t o extend the present methodology to full-surface applications is proposed.

Association between menthol-flavoured cigarette smoking and flavoured little cigar and cigarillo use among African-American, Hispanic, and white young and middle-aged adult smokers.

PubMed

Sterling, K; Fryer, C; Pagano, I; Jones, D; Fagan, P

2016-11-01

Flavour additives in cigarettes and little cigars and cigarillos (LCCs), which influence smokers' risk perceptions, may reinforce dual flavoured tobacco use. We examined the association among mentholated cigarette use, risk perceptions for flavour additives in LCCs and flavoured LCC smoking behaviour. Data from a national probability sample of 964 young and middle-aged adult current cigarette smokers were analysed. Multinomial logistic regression models examined the relationship among mentholated cigarette smoking, risk perceptions and current flavoured LCC use for the analytic sample and gender and race/ethnicity. Daily menthol cigarette smokers, compared to occasional, non-menthol smokers, had increased odds of flavoured LCC smoking (OR=1.75, 95% CI 1.02 to 2.98). This relationship was found for males, blacks/African-Americans and Hispanics/Latinos (p<0.05). Positive perceptions of menthol-flavoured additives in LCCs was associated with increased odds of flavoured LCC use among the analytic sample, males and blacks/African-Americans (p<0.05). Positive perceptions for clove-flavoured, spice-flavoured and alcohol-flavoured additives were also associated with flavoured LCC use among the analytic sample (p<0.05). Use of menthol-flavoured cigarettes and positive perceptions about menthol-flavoured and other flavour additives in LCCs may contribute to dual use with flavoured LCCs among adult cigarette smokers, specifically those from vulnerable populations. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/.

Mental Health Associate Program T-056: A Program Review, Spring 1991.

ERIC Educational Resources Information Center

Lenoir Community Coll., Kinston, NC.

At Lenoir Community College (LCC) in Kinston, North Carolina, 20% of established programs and 33% of the non-academic support services are reviewed each year in an annual cycle, with all programs reviewed within a 5-year period. To evaluate the Mental Health Associate (MHA) Program at LCC, assessment surveys were sent to 44 graduates from the…

Preparation and Transition: The Transfer Relationship between Lane Community College and the University of Oregon.

ERIC Educational Resources Information Center

Kempner, Ken; And Others

In 1991, a survey was sent to a random sample of University of Oregon (UO) students who had attended Lane Community College (LCC) between winter 1988 and spring 1990. The purpose of the survey was to investigate the perceptions and opinions of former LCC students concerning their community college and transfer experiences. Major findings, based on…

Power Systems for Future Missions: Appendices A-L

NASA Technical Reports Server (NTRS)

Gill, S. P.; Frye, P. E.; Littman, Franklin D.; Meisl, C. J.

1994-01-01

Selection of power system technology for space applications is typically based on mass, readiness of a particular technology to meet specific mission requirements, and life cycle costs (LCC). The LCC is typically used as a discriminator between competing technologies for a single mission application. All other future applications for a given technology are usually ignored. As a result, development cost of a technology becomes a dominant factor in the LCC comparison. Therefore, it is common for technologies such as DIPS and LMR-CBC to be potentially applicable to a wide range of missions and still lose out in the initial LCC comparison due to high development costs. This collection of appendices (A through L) contains the following power systems technology plans: CBC DIPS Technology Roadmap; PEM PFC Technology Roadmap; NAS Battery Technology Roadmap; PV/RFC Power System Technology Roadmap; PV/NAS Battery Technology Roadmap; Thermionic Reactor Power System Technology Roadmap; SP-100 Power System Technology Roadmap; Dynamic SP-100 Power System Technology Roadmap; Near-Term Solar Dynamic Power System Technology Roadmap; Advanced Solar Dynamic Power System Technology Roadmap; Advanced Stirling Cycle Dynamic Isotope Power System Technology Roadmap; and the ESPPRS (Evolutionary Space Power and Propulsion Requirements System) User's Guide.

Transition index maps for urban growth simulation: application of artificial neural networks, weight of evidence and fuzzy multi-criteria evaluation.

PubMed

Shafizadeh-Moghadam, Hossein; Tayyebi, Amin; Helbich, Marco

2017-06-01

Transition index maps (TIMs) are key products in urban growth simulation models. However, their operationalization is still conflicting. Our aim was to compare the prediction accuracy of three TIM-based spatially explicit land cover change (LCC) models in the mega city of Mumbai, India. These LCC models include two data-driven approaches, namely artificial neural networks (ANNs) and weight of evidence (WOE), and one knowledge-based approach which integrates an analytical hierarchical process with fuzzy membership functions (FAHP). Using the relative operating characteristics (ROC), the performance of these three LCC models were evaluated. The results showed 85%, 75%, and 73% accuracy for the ANN, FAHP, and WOE. The ANN was clearly superior compared to the other LCC models when simulating urban growth for the year 2010; hence, ANN was used to predict urban growth for 2020 and 2030. Projected urban growth maps were assessed using statistical measures, including figure of merit, average spatial distance deviation, producer accuracy, and overall accuracy. Based on our findings, we recomend ANNs as an and accurate method for simulating future patterns of urban growth.

Molecular Origin of Strength and Stiffness in Bamboo Fibrils.

PubMed

Youssefian, Sina; Rahbar, Nima

2015-06-08

Bamboo, a fast-growing grass, has a higher strength-to-weight ratio than steel and concrete. The unique properties of bamboo come from the natural composite structure of fibers that consists mainly of cellulose microfibrils in a matrix of intertwined hemicellulose and lignin called lignin-carbohydrate complex (LCC). Here, we have used atomistic simulations to study the mechanical properties of and adhesive interactions between the materials in bamboo fibers. With this aim, we have developed molecular models of lignin, hemicellulose and LCC structures to study the elastic moduli and the adhesion energies between these materials and cellulose microfibril faces. Good agreement was observed between the simulation results and experimental data. It was also shown that the hemicellulose model has stronger mechanical properties than lignin while lignin exhibits greater tendency to adhere to cellulose microfibrils. The study suggests that the abundance of hydrogen bonds in hemicellulose chains is responsible for improving the mechanical behavior of LCC. The strong van der Waals forces between lignin molecules and cellulose microfibril is responsible for higher adhesion energy between LCC and cellulose microfibrils. We also found out that the amorphous regions of cellulose microfibrils are the weakest interfaces in bamboo fibrils. Hence, they determine the fibril strength.

Molecular Origin of Strength and Stiffness in Bamboo Fibrils

PubMed Central

Youssefian, Sina; Rahbar, Nima

2015-01-01

Bamboo, a fast-growing grass, has a higher strength-to-weight ratio than steel and concrete. The unique properties of bamboo come from the natural composite structure of fibers that consists mainly of cellulose microfibrils in a matrix of intertwined hemicellulose and lignin called lignin-carbohydrate complex (LCC). Here, we have used atomistic simulations to study the mechanical properties of and adhesive interactions between the materials in bamboo fibers. With this aim, we have developed molecular models of lignin, hemicellulose and LCC structures to study the elastic moduli and the adhesion energies between these materials and cellulose microfibril faces. Good agreement was observed between the simulation results and experimental data. It was also shown that the hemicellulose model has stronger mechanical properties than lignin while lignin exhibits greater tendency to adhere to cellulose microfibrils. The study suggests that the abundance of hydrogen bonds in hemicellulose chains is responsible for improving the mechanical behavior of LCC. The strong van der Waals forces between lignin molecules and cellulose microfibril is responsible for higher adhesion energy between LCC and cellulose microfibrils. We also found out that the amorphous regions of cellulose microfibrils are the weakest interfaces in bamboo fibrils. Hence, they determine the fibril strength. PMID:26054045

Molecular Origin of Strength and Stiffness in Bamboo Fibrils

NASA Astrophysics Data System (ADS)

Youssefian, Sina; Rahbar, Nima

2015-06-01

Bamboo, a fast-growing grass, has a higher strength-to-weight ratio than steel and concrete. The unique properties of bamboo come from the natural composite structure of fibers that consists mainly of cellulose microfibrils in a matrix of intertwined hemicellulose and lignin called lignin-carbohydrate complex (LCC). Here, we have used atomistic simulations to study the mechanical properties of and adhesive interactions between the materials in bamboo fibers. With this aim, we have developed molecular models of lignin, hemicellulose and LCC structures to study the elastic moduli and the adhesion energies between these materials and cellulose microfibril faces. Good agreement was observed between the simulation results and experimental data. It was also shown that the hemicellulose model has stronger mechanical properties than lignin while lignin exhibits greater tendency to adhere to cellulose microfibrils. The study suggests that the abundance of hydrogen bonds in hemicellulose chains is responsible for improving the mechanical behavior of LCC. The strong van der Waals forces between lignin molecules and cellulose microfibril is responsible for higher adhesion energy between LCC and cellulose microfibrils. We also found out that the amorphous regions of cellulose microfibrils are the weakest interfaces in bamboo fibrils. Hence, they determine the fibril strength.

High Laccase Expression by Trametes versicolor in a Simulated Textile Effluent with Different Carbon Sources and PHs.

PubMed

Ottoni, Cristiane; Simões, Marta F; Fernandes, Sara; Santos, Cledir R; Lima, Nelson

2016-08-02

Textile effluents are highly polluting and have variable and complex compositions. They can be extremely complex, with high salt concentrations and alkaline pHs. A fixed-bed bioreactor was used in the present study to simulate a textile effluent treatment, where the white-rot fungus, Trametes versicolor, efficiently decolourised the azo dye Reactive Black 5 over 28 days. This occurred under high alkaline conditions, which is unusual, but advantageous, for successful decolourisation processes. Active dye decolourisation was maintained by operation in continuous culture. Colour was eliminated during the course of operation and maximum laccase (Lcc) activity (80.2 U∙L(-1)) was detected after glycerol addition to the bioreactor. Lcc2 gene expression was evaluated with different carbon sources and pH values based on reverse transcriptase-PCR (polymerase chain reaction). Glycerol was shown to promote the highest lcc2 expression at pH 5.5, followed by sucrose and then glucose. The highest levels of expression occurred between three and four days, which corroborate the maximum Lcc activity observed for sucrose and glycerol on the bioreactor. These results give new insights into the use of T. versicolor in textile dye wastewater treatment with high pHs.

Is Electromagnetic Gravity Control Possible?

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vargas, Jose G.; Torr, Douglas G.

2004-02-04

We study the interplay of Einstein's Gravitation (GR) and Maxwell's Electromagnetism, where the distribution of energy-momentum is not presently known (The Feynman Lectures, Vol 2, Chapter 27, section 4). As Feynman himself stated, one might in principle use Einstein's equations of GR to find such a distribution. GR (born in 1915) presently uses the Levi-Civita connection, LCC (the LCC was born two years after GR as a new concept, and not just as the pre-existing Christoffel symbols that represent it). Around 1927, Einstein proposed for physics an alternative to the LCC that constitutes a far more sensible and powerful affinemore » enrichment of metric Riemannian geometry. It is called teleparallelism (TP). Its Finslerian version (i.e. in the space-time-velocity arena) permits an unequivocal identification of the EM field as a geometric quantity. This in turn permits one to identify a completely geometric set of Einstein equations from curvature equations. From their right hand side, one may obtain the actual distribution of EM energy-momentum. It is consistent with Maxwell's equations, since these also are implied by the equations of structure of TP. We find that the so-far-unknown terms in this distribution amount to a total differential and do not, therefore, alter the value of the total EM energy-momentum. And yet these extra terms are at macroscopic distances enormously larger than the standard quadratic terms. This allows for the generation of measurable gravitational fields by EM fields. We thus answer affirmatively the question of the title.« less

Dissecting and Targeting Latent Metastasis

DTIC Science & Technology

2015-09-01

distinct class of stem-like cancer cells , which primed to enter quiescence and evade innate immunity after infiltrating distant organs. LCC cells express...state and actively silencing WNT signaling, LCC cells can enter quiescence and evade innate immunity to remain latent for extended periods. These...mutation in Foxn1 renders the mice athymic, severely blunting the maturation of effector T cells but preserving innate immunity components including

Malachite Green and Crystal Violet Decolorization by Ganoderma lucidum and Pleurotus ostreatus Supernatant and by rGlLCC1 and rPOXA 1B Concentrates: Molecular Docking Analysis.

PubMed

Morales-Álvarez, Edwin D; Rivera-Hoyos, Claudia M; Poveda-Cuevas, Sergio A; Reyes-Guzmán, Edwin A; Pedroza-Rodríguez, Aura M; Reyes-Montaño, Edgar A; Poutou-Piñales, Raúl A

2018-03-01

Laccases catalyze the oxidation of various aromatic organic compounds concomitantly with molecular oxygen reduction to water. Triphenylmethane dyes are synthetic compounds widely used in diverse industries. Their removal from effluents is difficult, due to their high degree of structural complexity; hence, their high concentration in effluents cause a negative impact on the environment. In the present work, molecular docking was used to evaluate interactions between rGlLCC1 or rPOXA 1B enzymes with Crystal Violet (CV) or Malachite Green (MG) dyes. In addition, removal tests of the two dyes were performed. Van der Waals interactions were obtained for only the CV dye for both GlLCC1 and POXA 1B enzymes. Nevertheless, in the GlLCC1 model, two π-π interactions were observed. For the MG dye only, Van der Waals interactions were obtained. Moreover, amino acid composition interacting in each model with each dye was similar. It is important to highlight that by molecular docking, none of the estimated ligand configurations generated hydrogen bonds. Thus, explaining the difficulty to degrade CV and MG. Regarding CV, maximum decolorization percentage was 23.6 ± 1.0% using Ganoderma lucidum supernatant and 5.0 ± 0.5% with Pleurotus ostreatus supernatant. When using recombinant laccase enzyme concentrates, decolorization percentages were 9.9 ± 0.1 and 7.5 ± 1.0% for rGlLCC1 and rPOXA 1B, respectively. On the other hand, for the MG dye, maximum decolorization percentages were 52.1 ± 5.1 and 2.3 ± 0.2% using G. lucidum and P. ostreatus concentrates, respectively. Whereas with recombinant laccase enzymatic concentrates, values of 9.4 ± 0.8% were obtained, with rGlLCC1, and 2.1 ± 0.1% when using rPOXA 1B. These findings represent an important step in bioremediation processes improvement and efficiency of industry-generated products, using environmentally friendly alternatives.

Clinical significance of circulating immune cells in left- and right-sided colon cancer.

PubMed

Di, Jiabo; Zhuang, Meng; Yang, Hong; Jiang, Beihai; Wang, Zaozao; Su, Xiangqian

2017-01-01

Left-sided and right-sided colon cancers (LCCs and RCCs, respectively) differ in their epidemiology, pathogenesis, genetic and epigenetic alterations, molecular pathways and prognosis. Notably, immune response gene expression profiles have been shown to differ between patients with LCC and patients with RCC. The immune system plays an important role in tumor immunosurveillance, and there is increasing evidence that peripheral blood immune cells have a profound influence on tumor prognosis. This study aimed to determine the clinical significance of circulating immune cells with respect to colon tumor locations. Different types of circulating immune cells were separated and analysed based on their surface markers by flow cytometry. We compared the numbers of dendritic cells (DCs) and T cell subsets in the peripheral blood of 94 patients with RCC or LCC and analysed the proportions of these immune cells in relation to tumor stage, tumor differentiation and lymphatic metastasis. We show that at later tumor stages, patients with LCC had higher levels of circulating myeloid DCs ( P  = 0.049) and plasmacytoid DCs ( P  = 0.018) than patients with RCC. In poorly differentiated tumors, LCC patients had significantly higher amount of plasmacytoid DCs ( P  = 0.036), CD4 + memory T (Tm) cells ( P  = 0.012), CD4 + T cells ( P  = 0.028), Tm cells ( P  = 0.014), and regulatory T cells ( P  = 0.001) than RCC patients. The levels of circulating CD4 + T cells, Tm cells and CD4 + Tm cells were significantly elevated at later stages in patients with LCC or RCC, while these cells decreased in poorly differentiated tumors in patients with RCC. Moreover, CD4 + Tm cell and CD4 + T cell levels are significantly associated with lymph node metastasis in patients with LCC and RCC. Circulating immune cells were associated with tumor location, tumor stage and tumor differentiation, and can be used to predict lymphatic metastasis in patients with colon cancer. This variation in systemic immunity could contribute to the differential prognosis of patients with colon cancer.

External Tank Liquid Hydrogen (LH2) Prepress Regression Analysis Independent Review Technical Consultation Report

NASA Technical Reports Server (NTRS)

Parsons, Vickie s.

2009-01-01

The request to conduct an independent review of regression models, developed for determining the expected Launch Commit Criteria (LCC) External Tank (ET)-04 cycle count for the Space Shuttle ET tanking process, was submitted to the NASA Engineering and Safety Center NESC on September 20, 2005. The NESC team performed an independent review of regression models documented in Prepress Regression Analysis, Tom Clark and Angela Krenn, 10/27/05. This consultation consisted of a peer review by statistical experts of the proposed regression models provided in the Prepress Regression Analysis. This document is the consultation's final report.

STS-35: Mission Highlights Resource Tape

NASA Technical Reports Server (NTRS)

1995-01-01

Live footage shows the crewmembers of STS-35, Commander Vance D. Brand, Pilot Guy S. Gardner, Mission Specialists Jeffrey A. Hoffman, John M. Lounge, and Robert A. Parker, and Payload Specialists Samuel T. Durrance, and Ronald A. Parise, participating in the traditional breakfast prior to launch. The crew is seen suiting up, and walking out to the Astro-Van for their 1 a.m. launch. Also shown are some beautiful panoramic shots of the shuttle on the launch pad, main engine start, ignition, liftoff, and various shots of the Launch Control Center (LCC). The crew is also shown during flight performing some routine functions such as operating the trash compactor, eating, and getting into and out of their sleeping quarters. The crew is seen taking part in a conversation with the Secretary of State, and the Foreign Minister of the Soviet Union. Footage also includes the landing of Columbia, its rollout on the runway, and its crew as they depart from the vehicle.

Right- and left-sided colon cancer – clinical and pathological differences of the disease entity in one organ

PubMed Central

Berut, Maciej; Dziki, Lukasz; Trzcinski, Radzislaw; Dziki, Adam

2016-01-01

Introduction Some researchers suggest that cancers located in the right vs. the left side of the colon are different and they can be regarded as distinct disease entities. The aim of this study was to analyze differences in clinical, epidemiological and pathological features of patients with right-sided (RCC) and left-sided (LCC) colon cancer. Material and methods One thousand two hundred and twenty-four patients were operated on due to colorectal cancer. A group of 477 patients (254 women, mean age 65.5 ±11 for the whole group) with colon cancer was included (212 RCC vs. 265 LCC). Results Right colon cancer patients were older (67.8 ±11.3 vs. 63.2 ±11.2; p = 0.0087). Left colon cancer patients underwent surgery for urgent indications more often (17.0% vs. 8.5%; p = 0006). Tumor diameter was greater in the RCC group (55 ±60 mm vs. 38 ±21 mm; p = 0.0003). Total number of removed lymph nodes was higher in the RCC group (11.7 ±6 vs. 8.3 ±5; p = 0.0001). Lymph node ratio was higher in the LCC group (0.45 ±0.28 vs. 0.30 ±0.25; p = 0.0063). We found a strong positive correlation between tumor diameter and the number of removed lymph nodes in the LCC group (r = 0.531). Conclusions These differences may result from the fact that RCC patients are diagnosed at an older age. The smaller number of removed lymph nodes in LCC patients may result in incorrect staging. It is still necessary to find other biological dissimilarities of adenocarcinoma located on different sides of the colon. PMID:28144267

Right- and left-sided colon cancer - clinical and pathological differences of the disease entity in one organ.

PubMed

Mik, Michal; Berut, Maciej; Dziki, Lukasz; Trzcinski, Radzislaw; Dziki, Adam

2017-02-01

Some researchers suggest that cancers located in the right vs. the left side of the colon are different and they can be regarded as distinct disease entities. The aim of this study was to analyze differences in clinical, epidemiological and pathological features of patients with right-sided (RCC) and left-sided (LCC) colon cancer. One thousand two hundred and twenty-four patients were operated on due to colorectal cancer. A group of 477 patients (254 women, mean age 65.5 ±11 for the whole group) with colon cancer was included (212 RCC vs. 265 LCC). Right colon cancer patients were older (67.8 ±11.3 vs. 63.2 ±11.2; p = 0.0087). Left colon cancer patients underwent surgery for urgent indications more often (17.0% vs. 8.5%; p = 0006). Tumor diameter was greater in the RCC group (55 ±60 mm vs. 38 ±21 mm; p = 0.0003). Total number of removed lymph nodes was higher in the RCC group (11.7 ±6 vs. 8.3 ±5; p = 0.0001). Lymph node ratio was higher in the LCC group (0.45 ±0.28 vs. 0.30 ±0.25; p = 0.0063). We found a strong positive correlation between tumor diameter and the number of removed lymph nodes in the LCC group ( r = 0.531). These differences may result from the fact that RCC patients are diagnosed at an older age. The smaller number of removed lymph nodes in LCC patients may result in incorrect staging. It is still necessary to find other biological dissimilarities of adenocarcinoma located on different sides of the colon.

Land-Use Change Impact on Soil Sustainability in a Climate and Vegetation Transition Zone

DOE Office of Scientific and Technical Information (OSTI.GOV)

Reitsma, Kurt D.; Dunn, Barry H.; Mishra, U.

A growing world population and climate change are expected to influence future agricultural productivity and land use. This study determined the impact of land-use change on soil sustainability and discussed the factors contributing to these changes. South Dakota was selected as a model system because corn ( Zea mays L.) grain prices tripled between 2006 and 2012 and it is located in a climate and grassland/cropland transition zone. High resolution imagery was used to visually determine land uses (cropland, grassland, nonagricultural, habitat, and water) at 14,400 points in 2006 and 2012. At each point, land-use change and the USDA landmore » capability class (LCC) were determined. Over the 6-yr study period, 6.87% of the grasslands (730,000 ha) were converted to cropland, with 93% occurring on lands generally considered suitable for crop production (LCC ≤ IV) if appropriate practices are followed. Converted grasslands, however, had higher LCC values than existing croplands and lower LCC values than remaining grasslands. In addition, 4.2% of the croplands (250,000 ha) were converted to grasslands, and statewide, 20,000 ha of croplands were converted to grasslands in areas limited by excess water (LCC V). The conversion of grasslands could not be linked to one specific factor and may be related to: (i) a desire to increase financial returns, (ii) changes in the land ownership structure, (iii) technology improvements, (iv) governmental policies, (v) climate change, and (vi) an aging workforce. Here, research and outreach programs that balance the goods and services of different land uses are needed to maintain sustainable agroecosystems.« less

Experimental Investigations into U/TRU Recovery using a Liquid Cadmium Cathode and Salt Containing High Rare Earth Concentrations

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shelly X. Li; Steven D. Herrmann; Michael F. Simpson

2009-09-01

Experimental Investigations into U/TRU Recovery using a Liquid Cadmium Cathode and Salt Containing High Rare Earth Concentrations Shelly X. Li, Steven D. Herrmann, and Michael F. Simpson Pyroprocessing Technology Department Idaho National Laboratory P.O. Box 1625, Idaho Falls, ID 83415 USA Abstract - A series of six bench-scale liquid cadmium cathode (LCC) tests was performed to obtain basic separation data with focus on the behavior of rare earth elements. The electrolyte used for the tests was a mixed salt from the Mk-IV and Mk-V electrorefiners, in which spent metal fuels from Experimental Breeder Reactor-II (EBR-II) had been processed. Rare earthmore » (RE) chlorides, such as NdCl3, CeCl3, LaCl3, PrCl3, SmCl3, and YCl3, were spiked into the salt prior to the first test to create an extreme case for investigating rare earth contamination of the actinides collected by a LCC. For the first two LCC tests, an alloy with the nominal composition of 41U-30Pu-5Am-3Np-20Zr-1RE was loaded into the anode baskets as the feed material. The anode feed material for Runs 3 to 6 was spent ternary fuel (U-19Pu-10Zr). The Pu/U ratio in the salt varied from 0.6 to 1.3. Chemical and radiochemical analytical results confirmed that U and transuranics can be collected into the LCC as a group under the given run conditions. The RE contamination level in the LCC product was up to 6.7 wt% of the total metal collected. The detailed data for partitioning of actinides and REs in the salt and Cd phases are reported in the paper.« less

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hoogmartens, Rob, E-mail: rob.hoogmartens@uhasselt.be; Van Passel, Steven, E-mail: steven.vanpassel@uhasselt.be; Van Acker, Karel, E-mail: karel.vanacker@lrd.kuleuven.be

Increasing interest in sustainability has led to the development of sustainability assessment tools such as Life Cycle Analysis (LCA), Life Cycle Costing (LCC) and Cost–Benefit Analysis (CBA). Due to methodological disparity of these three tools, conflicting assessment results generate confusion for many policy and business decisions. In order to interpret and integrate assessment results, the paper provides a framework that clarifies the connections and coherence between the included assessment methodologies. Building on this framework, the paper further focuses on key aspects to adapt any of the methodologies to full sustainability assessments. Aspects dealt with in the review are for examplemore » the reported metrics, the scope, data requirements, discounting, product- or project-related and approaches with respect to scarcity and labor requirements. In addition to these key aspects, the review shows that important connections exist: (i) the three tools can cope with social inequality, (ii) processes such as valuation techniques for LCC and CBA are common, (iii) Environmental Impact Assessment (EIA) is used as input in both LCA and CBA and (iv) LCA can be used in parallel with LCC. Furthermore, the most integrated sustainability approach combines elements of LCA and LCC to achieve the Life Cycle Sustainability Assessment (LCSA). The key aspects and the connections referred to in the review are illustrated with a case study on the treatment of end-of-life automotive glass. - Highlights: • Proliferation of assessment tools creates ambiguity and confusion. • The developed assessment framework clarifies connections between assessment tools. • Broadening LCA, key aspects are metric and data requirements. • Broadening LCC, key aspects are scope, time frame and discounting. • Broadening CBA, focus point, timespan, references, labor and scarcity are key.« less

Secretory expression of Lentinula edodes intracellular laccase by yeast high-cell-density system: sub-milligram production of difficult-to-express secretory protein.

PubMed

Kurose, Takeshi; Saito, Yuta; Kimata, Koichi; Nakagawa, Yuko; Yano, Akira; Ito, Keisuke; Kawarasaki, Yasuaki

2014-06-01

While a number of heterologous expression systems have been reported for extracellular laccases, there are few for the intracellular counterparts. The Lentinula edodes intracellular laccase Lcc4 is an industrially potential enzyme with its unique substrate specificity. The heterologous production of the intracellular laccase, however, had been difficult because of its expression-dependent toxicity. We previously demonstrated that recombinant yeast cells synthesized and, interestingly, secreted Lcc4 only when they were suspended to an inducing medium in a high cell-density (J. Biosci. Bioeng., 113, 154-159, 2012). The high cell-density system was versatile and applicable to other difficult-to-express secretory proteins. Nevertheless, the system's great dependence on aeration, which was a practical obstacle to scale-up production of the enzyme and some other proteins, left the secretion pathway and enzymatic properties of the Lcc4 uncharacterized. In this report, we demonstrate a successful production of Lcc4 by applying a jar-fermentor to the high cell-density system. The elevated yield (0.6 mg L(-1)) due to the sufficient aeration allowed us to prepare and purify the enzyme to homogeneity. The enzyme had been secreted as a hyper-glycosylated protein, resulting in smear band-formations in SDS-PAGE. The amino acid sequencing analysis suggested that the N-terminal 17 residues had been recognized as a secretion signal. The recombinant enzyme showed similar enzymatic properties to the naturally occurring Lcc4. The characteristics of the scale-upped expression system, which includes helpful information for the potential users, have also been described. Copyright © 2013 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

Equivalent Mass versus Life Cycle Cost for Life Support Technology Selection

NASA Technical Reports Server (NTRS)

Jones, Harry

2003-01-01

The decision to develop a particular life support technology or to select it for flight usually depends on the cost to develop and fly it. Other criteria - performance, safety, reliability, crew time, and risk - are considered, but cost is always an important factor. Because launch cost accounts for most of the cost of planetary missions, and because launch cost is directly proportional to the mass launched, equivalent mass has been used instead of cost to select life support technology. The equivalent mass of a life support system includes the estimated masses of the hardware and of the pressurized volume, power supply, and cooling system that the hardware requires. The equivalent mass is defined as the total payload launch mass needed to provide and support the system. An extension of equivalent mass, Equivalent System Mass (ESM), has been established for use in Advanced Life Support. A crew time mass-equivalent and sometimes other non-mass factors are added to equivalent mass to create ESM. Equivalent mass is an estimate of the launch cost only. For earth orbit rather than planetary missions, the launch cost is usually exceeded by the cost of Design, Development, Test, and Evaluation (DDT&E). Equivalent mass is used only in life support analysis. Life Cycle Cost (LCC) is much more commonly used. LCC includes DDT&E, launch, and operations costs. Since LCC includes launch cost, it is always a more accurate cost estimator than equivalent mass. The relative costs of development, launch, and operations vary depending on the mission design, destination, and duration. Since DDT&E or operations may cost more than launch, LCC may give a more accurate cost ranking than equivalent mass. To be sure of identifying the lowest cost technology for a particular mission, we should use LCC rather than equivalent mass.

Land-Use Change Impact on Soil Sustainability in a Climate and Vegetation Transition Zone

DOE PAGES

Reitsma, Kurt D.; Dunn, Barry H.; Mishra, U.; ...

2015-09-11

A growing world population and climate change are expected to influence future agricultural productivity and land use. This study determined the impact of land-use change on soil sustainability and discussed the factors contributing to these changes. South Dakota was selected as a model system because corn ( Zea mays L.) grain prices tripled between 2006 and 2012 and it is located in a climate and grassland/cropland transition zone. High resolution imagery was used to visually determine land uses (cropland, grassland, nonagricultural, habitat, and water) at 14,400 points in 2006 and 2012. At each point, land-use change and the USDA landmore » capability class (LCC) were determined. Over the 6-yr study period, 6.87% of the grasslands (730,000 ha) were converted to cropland, with 93% occurring on lands generally considered suitable for crop production (LCC ≤ IV) if appropriate practices are followed. Converted grasslands, however, had higher LCC values than existing croplands and lower LCC values than remaining grasslands. In addition, 4.2% of the croplands (250,000 ha) were converted to grasslands, and statewide, 20,000 ha of croplands were converted to grasslands in areas limited by excess water (LCC V). The conversion of grasslands could not be linked to one specific factor and may be related to: (i) a desire to increase financial returns, (ii) changes in the land ownership structure, (iii) technology improvements, (iv) governmental policies, (v) climate change, and (vi) an aging workforce. Here, research and outreach programs that balance the goods and services of different land uses are needed to maintain sustainable agroecosystems.« less

Policy shifts influence the functional changes of the CNH systems on the Mongolian plateau

NASA Astrophysics Data System (ADS)

Chen, Jiquan; John, Ranjeet; Shao, Changliang; Fan, Yi; Zhang, Yaoqi; Amarjargal, Amartuvshin; Brown, Daniel G.; Qi, Jiaguo; Han, Juanjuan; Lafortezza, Raffaele; Dong, Gang

2015-08-01

By applying the concept of the coupled natural and human system (CNH), we compared spatiotemporal changes in livestock (LSK), land cover, and ecosystem production to understand the relative roles that natural and social driving forces have on CNH dynamics on the Mongolia plateau. We used socioeconomic and physical data at prefecture level for Inner Mongolia and Mongolia from 1981 through 2010 to represent changes in net primary productivity (NPP), enhanced vegetation index (EVI), precipitation, annual average temperature, LSK, livestock density (LSKD), land cover change (LCC), gross domestic production (GDP), and population (POP). The ratios such as LSK:NPP, LSKD: EVI, LSKD:albedo, LSK:POP, and LSK:GDP were examined and compared between Inner Mongolia and Mongolia, and structural equation modeling (SEM) was applied to quantify the complex interactions. Substantial differences in LSK, POP, and economic development were found among the biomes and between Inner Mongolia and Mongolia. When various indicators for policy shifts—such as the World Trade Organization (WTO) for China, the Third Campaign to Reclaim Abandoned Agriculture Lands (ATAR-3), and the Grain for Green Program for China (GFG)—were added into our SEM, the results showed significant change in the strength of the above relationships. After China joined the WTO, the relationships in Inner Mongolia between LSKD:LCC and LSKD:NPP were immensely strengthened, whereas relationships in NPP:LCC were weakened. In Mongolia, the ATAR-3 program first appeared to be an insignificant policy, but the Collapse of the Soviet Union enhanced the correlation between LSKD:LCC, weakened the connection of LCC:NPP, and did not affect LSKD:NPP. We conclude that human influences on the Mongolian CNH system exceeded those of the biophysical changes, but that the significance varies in time and per biome, as well as between Inner Mongolia and Mongolia.

Host responses of Japanese flounder Paralichthys olivaceus with lymphocystis cell formation.

PubMed

Iwakiri, Shogo; Song, Jun-Young; Nakayama, Kei; Oh, Myung-Joo; Ishida, Minoru; Kitamura, Shin-Ichi

2014-06-01

Lymphocystis disease virus (LCDV) is the causative agent of lymphocystis disease (LCD). In this study, we investigated the mechanisms of lymphocystis cell (LCC) formation from the viewpoint of gene expression changes in the infected fish. LCC occurrence and virus titers in the experimentally infected Japanese flounder, Paralichthys olivaceus were monitored by visual confirmation and real-time PCR, respectively. The gene expression changes in the fish fin were investigated by microarray experiments. LCCs firstly appeared in the fish at 21 days post infection (dpi). LCD incidence increased with time and reached 92.9% at 62 dpi. LCDV genome was firstly detected from dorsal fins at 14 dpi, and the relative amount of the genome gradually-increased until 56 dpi. Since the occurrence of LCC was approximately synchronized with increasing of the virus genome, virus replication might play important roles for LCC formation. The microarray detected a few gene expression changes until 28 dpi. However, the number of expression changed genes dramatically increased between 28 and 42 dpi in which LCCs formation was active. From the microarray data analyses, apoptosis and cell division related genes were down-regulated, whereas cell fusion and collagen related genes were up-regulated at 42 dpi. Together with the observation of morphological changes of LCCs in previous reports, it is suggested that the following steps are involved in LCC formation: the virus infected cells were (1) inhibited apoptotic death and (2) cell division before enlargement, (3) hypertrophied by cell fusion, and (4) surrounded by a hyaline capsule associated with the alteration of collagen fibers. Copyright © 2014 Elsevier Ltd. All rights reserved.

Policies and Procedures for Bachelor of Business Administration Degree through the External Plan of Study from Northwood Institute, Midland, in Cooperation with Lansing Community College.

ERIC Educational Resources Information Center

Lansing Community Coll., MI.

Information is provided for students interested in completing a bachelor's degree through a cooperative program between Lansing (Michigan) Community College (LCC) and Northwood Institute (Midland, Michigan). A 4-year program has been designed in which a student takes approximately 144 credit hours of course work at LCC and a minimum of 36 credit…

Secretion of laccase and manganese peroxidase by Pleurotus strains cultivate in solid-state using Pinus spp. sawdust

PubMed Central

Camassola, Marli; da Rosa, Letícia O.; Calloni, Raquel; Gaio, Tamara A.; Dillon, Aldo J.P.

2013-01-01

Pleurotus species secrete phenol oxidase enzymes: laccase (Lcc) and manganese peroxidase (MnP). New genotypes of these species show potential to be used in processes aiming at the degradation of phenolic compounds, polycyclic aromatic hydrocarbons and dyes. Hence, a screening of some strains of Pleurotus towards Lcc and MnP production was performed in this work. Ten strains were grown through solid-state fermentation on a medium based on Pinus spp. sawdust, wheat bran and calcium carbonate. High Lcc and MnP activities were found with these strains. Highest Lcc activity, 741 ± 245 U gdm−1 of solid state-cultivation medium, was detected on strain IB11 after 32 days, while the highest MnP activity occurred with strains IB05, IB09, and IB11 (5,333 ± 357; 4,701 ± 652; 5,999 ± 1,078 U gdm−1, respectively). The results obtained here highlight the importance of further experiments with lignocellulolytic enzymes present in different strains of Pleurotus species. Such results also indicate the possibility of selecting more valuable strains for future biotechnological applications, in soil bioremediation and biological biomass pre-treatment in biofuels production, for instance, as well as obtaining value-added products from mushrooms, like phenol oxidase enzymes. PMID:24159307

High Laccase Expression by Trametes versicolor in a Simulated Textile Effluent with Different Carbon Sources and PHs

PubMed Central

Ottoni, Cristiane; Simões, Marta F.; Fernandes, Sara; Santos, Cledir R.; Lima, Nelson

2016-01-01

Textile effluents are highly polluting and have variable and complex compositions. They can be extremely complex, with high salt concentrations and alkaline pHs. A fixed-bed bioreactor was used in the present study to simulate a textile effluent treatment, where the white-rot fungus, Trametes versicolor, efficiently decolourised the azo dye Reactive Black 5 over 28 days. This occurred under high alkaline conditions, which is unusual, but advantageous, for successful decolourisation processes. Active dye decolourisation was maintained by operation in continuous culture. Colour was eliminated during the course of operation and maximum laccase (Lcc) activity (80.2 U∙L−1) was detected after glycerol addition to the bioreactor. Lcc2 gene expression was evaluated with different carbon sources and pH values based on reverse transcriptase-PCR (polymerase chain reaction). Glycerol was shown to promote the highest lcc2 expression at pH 5.5, followed by sucrose and then glucose. The highest levels of expression occurred between three and four days, which corroborate the maximum Lcc activity observed for sucrose and glycerol on the bioreactor. These results give new insights into the use of T. versicolor in textile dye wastewater treatment with high pHs. PMID:27490563

Historical land-use influences the long-term stream turbidity response to a wildfire.

PubMed

Harrison, Evan T; Dyer, Fiona; Wright, Daniel W; Levings, Chris

2014-02-01

Wildfires commonly result in an increase in stream turbidity. However, the influence of pre-fire land-use practices on post-fire stream turbidity is not well understood. The Lower Cotter Catchment (LCC) in south-eastern Australia is part of the main water supply catchment for Canberra with land in the catchment historically managed for a mix of conservation (native eucalypt forest) and pine (Pinus radiata) plantation. In January 2003, wildfires burned almost all of the native and pine forests in the LCC. A study was established in 2005 to determine stream post-fire turbidity recovery within the native and pine forest areas of the catchment. Turbidity data loggers were deployed in two creeks within burned native forest and burned pine forest areas to determine turbidity response to fire in these areas. As a part of the study, we also determined changes in bare soil in the native and pine forest areas since the fire. The results suggest that the time, it takes turbidity levels to decrease following wildfire, is dependent upon the preceding land-use. In the LCC, turbidity levels decreased more rapidly in areas previously with native vegetation compared to areas which were previously used for pine forestry. This is likely because of a higher percentage of bare soil areas for a longer period of time in the ex-pine forest estate and instream stores of fine sediment from catchment erosion during post-fire storm events. The results of our study show that the previous land-use may exert considerable control over on-going turbidity levels following a wildfire.

Induction of laccases in Trametes versicolor by aqueous wood extracts.

PubMed

Bertrand, Brandt; Martínez-Morales, Fernando; Tinoco, Raunel; Rojas-Trejo, Sonia; Serrano-Carreón, Leobardo; Trejo-Hernández, María R

2014-01-01

The induction of laccase isoforms in Trametes versicolor HEMIM-9 by aqueous extracts (AE) from softwood and hardwood was studied. Samples of sawdust of Pinus sp., Cedrela sp., and Quercus sp. were boiled in water to obtain AE. Different volumes of each AE were added to fungal cultures to determine the amount of AE needed for the induction experiments. Laccase activity was assayed every 24 h for 15 days. The addition of each AE (50 to 150 μl) to the fungal cultures increased laccase production compared to the control (0.42 ± 0.01 U ml(-1)). The highest laccase activities detected were 1.92 ± 0.15 U ml(-1) (pine), 1.87 ± 0.26 U ml(-1) (cedar), and 1.56 ± 0.34 U ml(-1) (oak); laccase productivities were also significantly increased. Larger volumes of any AE inhibited mycelial growth. Electrophoretic analysis revealed two laccase bands (lcc1 and lcc2) for all the treatments. However, when lcc2 was analyzed by isoelectric focusing, inducer-dependent isoform patterns composed of three (pine AE), four (oak AE), and six laccase bands (cedar AE) were observed. Thus, AE from softwood and hardwood had induction effects in T. versicolor HEMIM-9, as indicated by the increase in laccase activity and different isoform patterns. All of the enzymatic extracts were able to decolorize the dye Orange II. Dye decolorization was mainly influenced by pH. The optimum pH for decolorization was pH 5 (85%), followed by pH 7 (50%) and pH 3 (15%). No significant differences in the dye decolorizing capacity were detected between the control and the differentially induced laccase extracts (oak, pine and cedar). This could be due to the catalytic activities of isoforms with pI 5.4 and 5.8, which were detected under all induction conditions.

Computer Support for Conducting Supportability Trade-Offs in a Team Setting

DTIC Science & Technology

1990-01-01

maintenance visits, and spares costs. To minimize the total system LCC, which includes both acquisition and support costs, a method for obtaining the...from different departments with a range of skills to work for a common goal is not an easy task. Ignoring the logistical concerns, a fundamental problem...maintenance visits, and spares costs. To minimize the total system LCC, which includes both acquisition and support costs, a method for obtaining the

The relationship between interannual and long-term cloud feedbacks

DOE PAGES

Zhou, Chen; Zelinka, Mark D.; Dessler, Andrew E.; ...

2015-12-11

The analyses of Coupled Model Intercomparison Project phase 5 simulations suggest that climate models with more positive cloud feedback in response to interannual climate fluctuations also have more positive cloud feedback in response to long-term global warming. Ensemble mean vertical profiles of cloud change in response to interannual and long-term surface warming are similar, and the ensemble mean cloud feedback is positive on both timescales. However, the average long-term cloud feedback is smaller than the interannual cloud feedback, likely due to differences in surface warming pattern on the two timescales. Low cloud cover (LCC) change in response to interannual andmore » long-term global surface warming is found to be well correlated across models and explains over half of the covariance between interannual and long-term cloud feedback. In conclusion, the intermodel correlation of LCC across timescales likely results from model-specific sensitivities of LCC to sea surface warming.« less

Copula Entropy coupled with Wavelet Neural Network Model for Hydrological Prediction

NASA Astrophysics Data System (ADS)

Wang, Yin; Yue, JiGuang; Liu, ShuGuang; Wang, Li

2018-02-01

Artificial Neural network(ANN) has been widely used in hydrological forecasting. in this paper an attempt has been made to find an alternative method for hydrological prediction by combining Copula Entropy(CE) with Wavelet Neural Network(WNN), CE theory permits to calculate mutual information(MI) to select Input variables which avoids the limitations of the traditional linear correlation(LCC) analysis. Wavelet analysis can provide the exact locality of any changes in the dynamical patterns of the sequence Coupled with ANN Strong non-linear fitting ability. WNN model was able to provide a good fit with the hydrological data. finally, the hybrid model(CE+WNN) have been applied to daily water level of Taihu Lake Basin, and compared with CE ANN, LCC WNN and LCC ANN. Results showed that the hybrid model produced better results in estimating the hydrograph properties than the latter models.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhou, Chen; Zelinka, Mark D.; Dessler, Andrew E.

The analyses of Coupled Model Intercomparison Project phase 5 simulations suggest that climate models with more positive cloud feedback in response to interannual climate fluctuations also have more positive cloud feedback in response to long-term global warming. Ensemble mean vertical profiles of cloud change in response to interannual and long-term surface warming are similar, and the ensemble mean cloud feedback is positive on both timescales. However, the average long-term cloud feedback is smaller than the interannual cloud feedback, likely due to differences in surface warming pattern on the two timescales. Low cloud cover (LCC) change in response to interannual andmore » long-term global surface warming is found to be well correlated across models and explains over half of the covariance between interannual and long-term cloud feedback. In conclusion, the intermodel correlation of LCC across timescales likely results from model-specific sensitivities of LCC to sea surface warming.« less

Influence of pectins on the solubility and the molar mass distribution of dehydrogenative polymers (DHPs, lignin model compounds).

PubMed

Cathala, B; Monties, B

2001-07-19

Dehydrogenation polymers (DHPs, lignin model compounds) were synthesized in the presence of increasing pectin concentrations using two different methods. The first method ('Zutropfverfahren', ZT) consists in the slow adding of monomers whereas in the second method ('Zulaufverfahren', ZL) all the reactants are added simultaneously. DHPs solubility increases with the pectin concentration in the ZT experiments and remains stable in the ZL experiments. Covalent bonds between pectin and DHP are formed during ZT polymerization resulting in lignin carbohydrate complex (LCC) which keeps the unbound DHPs in solution by the formation of aggregate or micelle-like structures. In contrast LCC are not formed during the ZL process which behave like the DHP reference. The ZT DHP molar masses increase observed is attributed to the reactivity of the high molar mass polymer solubilized by the LCC whereas ZL higher molar mass polymers are precipitated out of the solution and cannot react further.

Uncertainty analysis and global sensitivity analysis of techno-economic assessments for biodiesel production.

PubMed

Tang, Zhang-Chun; Zhenzhou, Lu; Zhiwen, Liu; Ningcong, Xiao

2015-01-01

There are various uncertain parameters in the techno-economic assessments (TEAs) of biodiesel production, including capital cost, interest rate, feedstock price, maintenance rate, biodiesel conversion efficiency, glycerol price and operating cost. However, fewer studies focus on the influence of these parameters on TEAs. This paper investigated the effects of these parameters on the life cycle cost (LCC) and the unit cost (UC) in the TEAs of biodiesel production. The results show that LCC and UC exhibit variations when involving uncertain parameters. Based on the uncertainty analysis, three global sensitivity analysis (GSA) methods are utilized to quantify the contribution of an individual uncertain parameter to LCC and UC. The GSA results reveal that the feedstock price and the interest rate produce considerable effects on the TEAs. These results can provide a useful guide for entrepreneurs when they plan plants. Copyright © 2014 Elsevier Ltd. All rights reserved.

Modeling Impacts of Climate and Land Use Change on Ecosystem Processes to Quantify Exposure to Climate Change in Two Landscape Conservation Cooperatives

NASA Astrophysics Data System (ADS)

Quackenbush, A.

2015-12-01

Urban land cover and associated impervious surface area is expected to increase by as much as 50% over the next few decades across substantial portions of the United States. In combination with urban expansion, increases in temperature and changes in precipitation are expected to impact ecosystems through changes in productivity, disturbance and hydrological properties. In this study, we use the NASA Terrestrial Observation and Prediction System Biogeochemical Cycle (TOPS-BGC) model to explore the combined impacts of urbanization and climate change on hydrologic dynamics (snowmelt, runoff, and evapotranspiration) and vegetation carbon uptake (gross productivity). The model is driven using land cover predictions from the Spatially Explicit Regional Growth Model (SERGoM) to quantify projected changes in impervious surface area, and climate projections from the 30 arc-second NASA Earth Exchange Downscaled Climate Projection (NEX-DCP30) dataset derived from the CMIP5 climate scenarios. We present the modeling approach and an analysis of the ecosystem impacts projected to occur in the US, with an emphasis on protected areas in the Great Northern and Appalachian Landscape Conservation Cooperatives (LCC). Under the ensemble average of the CMIP5 models and land cover change scenarios for both representative concentration pathways (RCPs) 4.5 and 8.5, both LCCs are predicted to experience increases in maximum and minimum temperatures as well as annual average precipitation. In the Great Northern LCC, this is projected to lead to increased annual runoff, especially under RCP 8.5. Earlier melt of the winter snow pack and increased evapotranspiration, however, reduces summer streamflow and soil water content, leading to a net reduction in vegetation productivity across much of the Great Northern LCC, with stronger trends occurring under RCP 8.5. Increased runoff is also projected to occur in the Appalachian LCC under both RCP 4.5 and 8.5. However, under RCP 4.5, the model predicts that the warmer wetter conditions will lead to increases in vegetation productivity across much of the Appalachian LCC, while under RCP 8.5, the effects of increased precipitation are not enough to keep up with increases in evapotranspiration, leading to projected reductions in vegetation productivity for this LCC by the end of this century.

LCC-Demons: a robust and accurate symmetric diffeomorphic registration algorithm.

PubMed

Lorenzi, M; Ayache, N; Frisoni, G B; Pennec, X

2013-11-01

Non-linear registration is a key instrument for computational anatomy to study the morphology of organs and tissues. However, in order to be an effective instrument for the clinical practice, registration algorithms must be computationally efficient, accurate and most importantly robust to the multiple biases affecting medical images. In this work we propose a fast and robust registration framework based on the log-Demons diffeomorphic registration algorithm. The transformation is parameterized by stationary velocity fields (SVFs), and the similarity metric implements a symmetric local correlation coefficient (LCC). Moreover, we show how the SVF setting provides a stable and consistent numerical scheme for the computation of the Jacobian determinant and the flux of the deformation across the boundaries of a given region. Thus, it provides a robust evaluation of spatial changes. We tested the LCC-Demons in the inter-subject registration setting, by comparing with state-of-the-art registration algorithms on public available datasets, and in the intra-subject longitudinal registration problem, for the statistically powered measurements of the longitudinal atrophy in Alzheimer's disease. Experimental results show that LCC-Demons is a generic, flexible, efficient and robust algorithm for the accurate non-linear registration of images, which can find several applications in the field of medical imaging. Without any additional optimization, it solves equally well intra & inter-subject registration problems, and compares favorably to state-of-the-art methods. Copyright © 2013 Elsevier Inc. All rights reserved.

Comparative life cycle cost assessment of painted and hot-dip galvanized bridges.

PubMed

Rossi, B; Marquart, S; Rossi, G

2017-07-15

The study addresses the life cycle cost assessment (LCCA) of steel bridges, focusing on the maintenance activities and the maintenance scenario. Firstly, the unit costs of maintenance activities and their durability (i.e. the time between two activities) are evaluated. Pragmatic data are provided for the environment category C4 and for three activities: Patch Up, Overcoating and Remove & Replace. A comparative LCCA for a typical hypothetic steel girder bridge is carried out, either painted or hot-dip galvanized (HDG), in the environmental class C4. The LCC versus the cumulated life is provided for both options. The initial cost of the steel unpainted option is only 50.3% of the HDG option. It is shown that after 'Overcoating' occurring at 18.5 years, the total Net Present Value (NPV) of the painted option surpasses that of the HDG option. A sensitivity analysis of the NPV to the cost and service life parameters, the escalation and discount rates is then performed. The discount and escalation rates, considerably influences the total LCC, following a non-linear trend. The total LCC decreases with the discount rate increasing and, conversely, increases with the escalation rate increasing. Secondly, the influence of the maintenance scenario on the total LCC is assessed based on a probabilistic approach. A permutation of the three independent maintenance activities assumed to occur six times over the life of the bridge is considered and a probability of occurrence is associated to each unique scenario. The most probable scenarios are then classified according to their NPV or achieved service life. This approach leads to the definition of a cost-effective maintenance scenario i.e. the scenario, within all the considered permutations, that has the minimum LCC in a range of lifespan. Besides, the probabilistic analysis also shows that, whatever the scenario, the return on investment period ranges between 18.5 years and 24.2 years. After that period, the HDG option becomes economic. Copyright © 2017 Elsevier Ltd. All rights reserved.

β-D-glucan inhibits endocrine-resistant breast cancer cell proliferation and alters gene expression

PubMed Central

JAFAAR, ZAINAB M.T.; LITCHFIELD, LACEY M.; IVANOVA, MARGARITA M.; RADDE, BRANDIE N.; AL-RAYYAN, NUMAN; KLINGE, CAROLYN M.

2014-01-01

Endocrine therapies have been successfully used for breast cancer patients with estrogen receptor α (ERα) positive tumors, but ∼40% of patients relapse due to endocrine resistance. β-glucans are components of plant cell walls that have immunomodulatory and anticancer activity. The objective of this study was to examine the activity of β-D-glucan, purified from barley, in endocrine-sensitive MCF-7 versus endocrine-resistant LCC9 and LY2 breast cancer cells. β-D-glucan dissolved in DMSO but not water inhibited MCF-7 cell proliferation in a concentration-dependent manner as measured by BrdU incorporation with an IC50 of ∼164±12 μg/ml. β-D-glucan dissolved in DMSO inhibited tamoxifen/endocrine-resistant LCC9 and LY2 cell proliferation with IC50 values of 4.6±0.3 and 24.2±1.4 μg/ml, respectively. MCF-10A normal breast epithelial cells showed a higher IC50 ∼464 μg/ml and the proliferation of MDA-MB-231 triple negative breast cancer cells was not inhibited by β-D-glucan. Concentration-dependent increases in the BAX/BCL2 ratio and cell death with β-D-glucan were observed in MCF-7 and LCC9 cells. PCR array analysis revealed changes in gene expression in response to 24-h treatment with 10 or 50 μg/ml β-D-glucan that were different between MCF-7 and LCC9 cells as well as differences in basal gene expression between the two cell lines. Select results were confirmed by quantitative real-time PCR demonstrating that β-D-glucan increased RASSF1 expression in MCF-7 cells and IGFBP3, CTNNB1 and ERβ transcript expression in LCC9 cells. Our data indicate that β-D-glucan regulates breast cancer-relevant gene expression and may be useful for inhibiting endocrine-resistant breast cancer cell proliferation. PMID:24534923

Optimization of a one-step heat-inducible in vivo mini DNA vector production system.

PubMed

Nafissi, Nafiseh; Sum, Chi Hong; Wettig, Shawn; Slavcev, Roderick A

2014-01-01

While safer than their viral counterparts, conventional circular covalently closed (CCC) plasmid DNA vectors offer a limited safety profile. They often result in the transfer of unwanted prokaryotic sequences, antibiotic resistance genes, and bacterial origins of replication that may lead to unwanted immunostimulatory responses. Furthermore, such vectors may impart the potential for chromosomal integration, thus potentiating oncogenesis. Linear covalently closed (LCC), bacterial sequence free DNA vectors have shown promising clinical improvements in vitro and in vivo. However, the generation of such minivectors has been limited by in vitro enzymatic reactions hindering their downstream application in clinical trials. We previously characterized an in vivo temperature-inducible expression system, governed by the phage λ pL promoter and regulated by the thermolabile λ CI[Ts]857 repressor to produce recombinant protelomerase enzymes in E. coli. In this expression system, induction of recombinant protelomerase was achieved by increasing culture temperature above the 37°C threshold temperature. Overexpression of protelomerase led to enzymatic reactions, acting on genetically engineered multi-target sites called "Super Sequences" that serve to convert conventional CCC plasmid DNA into LCC DNA minivectors. Temperature up-shift, however, can result in intracellular stress responses and may alter plasmid replication rates; both of which may be detrimental to LCC minivector production. We sought to optimize our one-step in vivo DNA minivector production system under various induction schedules in combination with genetic modifications influencing plasmid replication, processing rates, and cellular heat stress responses. We assessed different culture growth techniques, growth media compositions, heat induction scheduling and temperature, induction duration, post-induction temperature, and E. coli genetic background to improve the productivity and scalability of our system, achieving an overall LCC DNA minivector production efficiency of ∼ 90%.We optimized a robust technology conferring rapid, scalable, one-step in vivo production of LCC DNA minivectors with potential application to gene transfer-mediated therapeutics.

Optimization of a One-Step Heat-Inducible In Vivo Mini DNA Vector Production System

PubMed Central

Wettig, Shawn; Slavcev, Roderick A.

2014-01-01

While safer than their viral counterparts, conventional circular covalently closed (CCC) plasmid DNA vectors offer a limited safety profile. They often result in the transfer of unwanted prokaryotic sequences, antibiotic resistance genes, and bacterial origins of replication that may lead to unwanted immunostimulatory responses. Furthermore, such vectors may impart the potential for chromosomal integration, thus potentiating oncogenesis. Linear covalently closed (LCC), bacterial sequence free DNA vectors have shown promising clinical improvements in vitro and in vivo. However, the generation of such minivectors has been limited by in vitro enzymatic reactions hindering their downstream application in clinical trials. We previously characterized an in vivo temperature-inducible expression system, governed by the phage λ pL promoter and regulated by the thermolabile λ CI[Ts]857 repressor to produce recombinant protelomerase enzymes in E. coli. In this expression system, induction of recombinant protelomerase was achieved by increasing culture temperature above the 37°C threshold temperature. Overexpression of protelomerase led to enzymatic reactions, acting on genetically engineered multi-target sites called “Super Sequences” that serve to convert conventional CCC plasmid DNA into LCC DNA minivectors. Temperature up-shift, however, can result in intracellular stress responses and may alter plasmid replication rates; both of which may be detrimental to LCC minivector production. We sought to optimize our one-step in vivo DNA minivector production system under various induction schedules in combination with genetic modifications influencing plasmid replication, processing rates, and cellular heat stress responses. We assessed different culture growth techniques, growth media compositions, heat induction scheduling and temperature, induction duration, post-induction temperature, and E. coli genetic background to improve the productivity and scalability of our system, achieving an overall LCC DNA minivector production efficiency of ∼90%.We optimized a robust technology conferring rapid, scalable, one-step in vivo production of LCC DNA minivectors with potential application to gene transfer-mediated therapeutics. PMID:24586704

Sol-gel derived (La 0.8M 0.2)CrO 3 (M dbnd Ca, Sr) coating layer on stainless-steel substrate for use as a separator in intermediate-temperature solid oxide fuel cell

NASA Astrophysics Data System (ADS)

A Lee, E.; Lee, S.; Hwang, H. J.; Moon, J.-W.

A ceramic coating technique is applied to reduce the voltage drop caused by oxidation of the metallic separator (SUS444) in intermediate-temperature (IT) solid oxide fuel cell (SOFCs) systems. Precursor solutions for (La, Ca)CrO 3 (LCC) and (La, Sr)CrO 3 (LSC) coatings are prepared by adding nitric acid and ethylene glycol into an aqueous solution of lanthanum, strontium (or calcium) and chromium nitrates. Dried LCC and LSC gel films are heat-treated at 400-800 °C after dip-coating on the SUS444 substrate. XRD and Fourier-transform infrared (FT-IR) analysis is used to examine the crystallization behaviour and chemical structure of the precursor solution. The oxidation behaviour of the coated SUS444 substrate is compared with an uncoated SUS444 substrate. The oxidation of the SUS444 is inhibited by the LCC and LSC thin film layers.

Systems integration and demonstration of advanced reusable structure for ALS

NASA Technical Reports Server (NTRS)

Gibbins, Martin N.

1991-01-01

The objective was to investigate the potential of advanced material to achieve life cycle cost (LCC) benefits for reusable structure on the advanced launch system. Three structural elements were investigated - all components of an Advanced Launch System reusable propulsion/avionics module. Leading aeroshell configurations included sandwich structure using titanium, graphite/polyimide (Gr/PI), or high-temperature aluminum (HTA) face sheets. Thrust structure truss concepts used titanium, graphite/epoxy, or silicon carbide/aluminum struts. Leading aft bulkhead concepts employed graphite epoxy and aluminum. The technical effort focused on the aeroshell because the greatest benefits were expected there. Thermal analyses show the structural temperature profiles during operation. Finite element analyses show stresses during splash-down. Weight statements and manufacturing cost estimates were prepared for calculation of LCC for each design. The Gr/PI aeroshell showed the lowest potential LCC, but the HTA aeroshell was judged to be lower risk. A technology development plan was prepared to validate the applicable structural technology.

Modelling of concrete topping thickness effects on the vibration behaviour for lvl-concrete composite floor (LCC)

NASA Astrophysics Data System (ADS)

Ghafar, NH Abd; Sahban, N. M.

2017-11-01

This research was conducted on 2 m LVL - concrete composite (LCC) floor consisting of two parts between concrete floor and laminated veneer lumber (LVL) timber joist. The floor system was model using SAP 2000 software package. The aim of this research to study the vibration behaviour of the LCC floor with different concrete topping thickness which 25 mm, 65 mm and from 20 mm until 200 mm in every 20 mm interval. Natural frequency decision produced through SAP 2000 in thickness 25 mm and 65 mm is 57.45 Hz and 57.19 Hz. In thickness from 20 mm until 200 mm in every 20 mm interval, optimum value which found is during thickness reach 65 mm. For concrete topping below 65 mm thickness, the mass will be domain the behavior of the floor. When concrete topping increased more than 65 mm, the behavior of the floor will be domain by floor stiffness.

Comparative study of superconducting fault current limiter both for LCC-HVDC and VSC-HVDC systems

NASA Astrophysics Data System (ADS)

Lee, Jong-Geon; Khan, Umer Amir; Lim, Sung-Woo; Shin, Woo-ju; Seo, In-Jin; Lee, Bang-Wook

2015-11-01

High Voltage Direct Current (HVDC) system has been evaluated as the optimum solution for the renewable energy transmission and long-distance power grid connections. In spite of the various advantages of HVDC system, it still has been regarded as an unreliable system compared to AC system due to its vulnerable characteristics on the power system fault. Furthermore, unlike AC system, optimum protection and switching device has not been fully developed yet. Therefore, in order to enhance the reliability of the HVDC systems mitigation of power system fault and reliable fault current limiting and switching devices should be developed. In this paper, in order to mitigate HVDC fault, both for Line Commutated Converter HVDC (LCC-HVDC) and Voltage Source Converter HVDC (VSC-HVDC) system, an application of resistive superconducting fault current limiter which has been known as optimum solution to cope with the power system fault was considered. Firstly, simulation models for two types of LCC-HVDC and VSC-HVDC system which has point to point connection model were developed. From the designed model, fault current characteristics of faulty condition were analyzed. Second, application of SFCL on each types of HVDC system and comparative study of modified fault current characteristics were analyzed. Consequently, it was deduced that an application of AC-SFCL on LCC-HVDC system with point to point connection was desirable solution to mitigate the fault current stresses and to prevent commutation failure in HVDC electric power system interconnected with AC grid.

Operations and support cost modeling using Markov chains

NASA Technical Reports Server (NTRS)

Unal, Resit

1989-01-01

Systems for future missions will be selected with life cycle costs (LCC) as a primary evaluation criterion. This reflects the current realization that only systems which are considered affordable will be built in the future due to the national budget constaints. Such an environment calls for innovative cost modeling techniques which address all of the phases a space system goes through during its life cycle, namely: design and development, fabrication, operations and support; and retirement. A significant portion of the LCC for reusable systems are generated during the operations and support phase (OS). Typically, OS costs can account for 60 to 80 percent of the total LCC. Clearly, OS costs are wholly determined or at least strongly influenced by decisions made during the design and development phases of the project. As a result OS costs need to be considered and estimated early in the conceptual phase. To be effective, an OS cost estimating model needs to account for actual instead of ideal processes by associating cost elements with probabilities. One approach that may be suitable for OS cost modeling is the use of the Markov Chain Process. Markov chains are an important method of probabilistic analysis for operations research analysts but they are rarely used for life cycle cost analysis. This research effort evaluates the use of Markov Chains in LCC analysis by developing OS cost model for a hypothetical reusable space transportation vehicle (HSTV) and suggests further uses of the Markov Chain process as a design-aid tool.

Evaluation of Externality Costs in Life-Cycle Optimization of Municipal Solid Waste Management Systems.

PubMed

Martinez-Sanchez, Veronica; Levis, James W; Damgaard, Anders; DeCarolis, Joseph F; Barlaz, Morton A; Astrup, Thomas F

2017-03-21

The development of sustainable solid waste management (SWM) systems requires consideration of both economic and environmental impacts. Societal life-cycle costing (S-LCC) provides a quantitative framework to estimate both economic and environmental impacts, by including "budget costs" and "externality costs". Budget costs include market goods and services (economic impact), whereas externality costs include effects outside the economic system (e.g., environmental impact). This study demonstrates the applicability of S-LCC to SWM life-cycle optimization through a case study based on an average suburban U.S. county of 500 000 people generating 320 000 Mg of waste annually. Estimated externality costs are based on emissions of CO 2 , CH 4 , N 2 O, PM 2.5 , PM 10 , NO x , SO 2 , VOC, CO, NH 3 , Hg, Pb, Cd, Cr (VI), Ni, As, and dioxins. The results indicate that incorporating S-LCC into optimized SWM strategy development encourages the use of a mixed waste material recovery facility with residues going to incineration, and separated organics to anaerobic digestion. Results are sensitive to waste composition, energy mix and recycling rates. Most of the externality costs stem from SO 2 , NO x , PM 2.5 , CH 4 , fossil CO 2 , and NH 3 emissions. S-LCC proved to be a valuable tool for policy analysis, but additional data on key externality costs such as organic compounds emissions to water would improve future analyses.

Life Cycle Cost Management, Methodology, and Case Studies

DTIC Science & Technology

1977-10-01

PAGES60 14. MONITORING AGENCY NAME G ADORESS(If different from Controlling Office) IS. SECURITY CLASS. (of this report) UNCLASSIFIED 150. DECLASSI FI...DISTRIBUTION STATEMENT (of thA abstract entered In Block 20, It different from Report) I8. SUPPLEMEINTARY NOTES ,.,IOUNCEI 0 Uy...... ....... 1. KEY WORDS...to provide the reader with an overview of the guidelines content and a preview of CAIG O&S costing method - ology .:,idelines. The RzMD report on LCC

Life cycle costing of waste management systems: Overview, calculation principles and case studies

DOE Office of Scientific and Technical Information (OSTI.GOV)

Martinez-Sanchez, Veronica, E-mail: vems@env.dtu.dk; Kromann, Mikkel A.; Astrup, Thomas Fruergaard

2015-02-15

Highlights: • We propose a comprehensive model for cost assessment of waste management systems. • The model includes three types of LCC: Conventional, Environmental and Societal LCCs. • The applicability of the proposed model is tested with two case studies. - Abstract: This paper provides a detailed and comprehensive cost model for the economic assessment of solid waste management systems. The model was based on the principles of Life Cycle Costing (LCC) and followed a bottom-up calculation approach providing detailed cost items for all key technologies within modern waste systems. All technologies were defined per tonne of waste input, andmore » each cost item within a technology was characterised by both a technical and an economic parameter (for example amount and cost of fuel related to waste collection), to ensure transparency, applicability and reproducibility. Cost items were classified as: (1) budget costs, (2) transfers (for example taxes, subsidies and fees) and (3) externality costs (for example damage or abatement costs related to emissions and disamenities). Technology costs were obtained as the sum of all cost items (of the same type) within a specific technology, while scenario costs were the sum of all technologies involved in a scenario. The cost model allows for the completion of three types of LCC: a Conventional LCC, for the assessment of financial costs, an Environmental LCC, for the assessment of financial costs whose results are complemented by a Life Cycle Assessment (LCA) for the same system, and a Societal LCC, for socio-economic assessments. Conventional and Environmental LCCs includes budget costs and transfers, while Societal LCCs includes budget and externality costs. Critical aspects were found in the existing literature regarding the cost assessment of waste management, namely system boundary equivalency, accounting for temporally distributed emissions and impacts, inclusions of transfers, the internalisation of environmental impacts and the coverage of shadow prices, and there was also significant confusion regarding terminology. The presented cost model was implemented in two case study scenarios assessing the costs involved in the source segregation of organic waste from 100,000 Danish households and the subsequent co-digestion of organic waste with animal manure. Overall, source segregation resulted in higher financial costs than the alternative of incinerating the organic waste with the residual waste: 1.6 M€/year, of which 0.9 M€/year was costs for extra bins and bags used by the households, 1.0 M€/year for extra collections and −0.3 M€/year saved on incineration.« less

White matter fiber integrity of the saccadic eye movement network differs between schizophrenia and healthy groups.

PubMed

Schaeffer, David J; Rodrigue, Amanda L; Burton, Courtney R; Pierce, Jordan E; Murphy, Megan N; Clementz, Brett A; McDowell, Jennifer E

2017-12-01

Recent diffusion tensor imaging (DTI) studies suggest that altered white matter fiber integrity is a pathophysiological feature of schizophrenia. Lower white matter integrity is associated with poor cognitive control, a characteristic of schizophrenia that can be measured using antisaccade tasks. Although the functional neural correlates of poor antisaccade performance have been well documented, fewer studies have investigated the extent to which white matter fibers connecting the functional nodes of this network contribute to antisaccade performance. The aim of the present study was to assess the white matter structural integrity of fibers connecting two functional nodes (putamen and medial frontal eye fields) of the saccadic eye movement network implicated in poor antisaccade performance in schizophrenia. To evaluate white matter integrity, DTI was acquired on subjects with schizophrenia and two comparison groups: (a) behaviorally matched healthy comparison subjects with low levels of cognitive control (LCC group), and (b) healthy subjects with high levels of cognitive control (HCC group). White matter fibers were tracked between functional regions of interest generated from antisaccade fMRI activation maps, and measures of diffusivity were quantified. The results demonstrated lower white matter integrity in the schizophrenia group than in the HCC group, but not the LCC group who showed similarly poor cognitive control performance. Overall, the results suggest that these alterations are not specific to the disease process of schizophrenia, but may rather be a function of uncontrolled cognitive factors that are concomitant with the disease but also observed in some healthy people. © 2017 Society for Psychophysiological Research.

Supporting Multiple Programs and Projects at NASA's Kennedy Space Center

NASA Technical Reports Server (NTRS)

Stewart, Camiren L.

2014-01-01

With the conclusion of the shuttle program in 2011, the National Aeronautics and Space Administration (NASA) had found itself at a crossroads for finding transportation of United States astronauts and experiments to space. The agency would eventually hand off the taxiing of American astronauts to the International Space Station (ISS) that orbits in Low Earth Orbit (LEO) about 210 miles above the earth under the requirements of the Commercial Crew Program (CCP). By privatizing the round trip journey from Earth to the ISS, the space agency has been given the additional time to focus funding and resources to projects that operate beyond LEO; however, adding even more stress to the agency, the premature cancellation of the program that would succeed the Shuttle Program - The Constellation Program (CxP) -it would inevitably delay the goal to travel beyond LEO for a number of years. Enter the Space Launch System (SLS) and the Orion Multipurpose Crew Vehicle (MPCV). Currently, the SLS is under development at NASA's Marshall Spaceflight Center in Huntsville, Alabama, while the Orion Capsule, built by government contractor Lockheed Martin Corporation, has been assembled and is currently under testing at the Kennedy Space Center (KSC) in Florida. In its current vision, SLS will take Orion and its crew to an asteroid that had been captured in an earlier mission in lunar orbit. Additionally, this vehicle and its configuration is NASA's transportation to Mars. Engineers at the Kennedy Space Center are currently working to test the ground systems that will facilitate the launch of Orion and the SLS within its Ground Services Development and Operations (GSDO) Program. Firing Room 1 in the Launch Control Center (LCC) has been refurbished and outfitted to support the SLS Program. In addition, the Spaceport Command and Control System (SCCS) is the underlying control system for monitoring and launching manned launch vehicles. As NASA finds itself at a junction, so does all of its associated centers across the US. KSC has found itself at the blunt end of change as the entire center has transitioned from an operations mindset to a development mentality. The author of this paper has had the fortunate privilege and opportunity to be part of a transforming NASA during the fall months of 2014. The following is a high level account of projects that he had the chance to work on including the Spaceport Command and Control System, the Advanced Ground System and Maintenance Program Project, Customer Avionics Development & Analysis (CAIDA) Lab and Swamp Works.

Personnel Data Analysis and Retrieval of Phase 1 Move To LC-39 Area

NASA Technical Reports Server (NTRS)

Davis, Derrick D.

2013-01-01

As a technology major from Jackson State University (JSU) I was called in as a summer intern at Kennedy Space Center (KSC) to work in the NASA Engineering, Control and Data Systems (NE-C) Division supporting the Spaceport Command and Control System (SCCS) at the Space Station Processing Facility (SSPF). I was given a two-part project; the first consisted of lending support relocating SCCS Computer Equipment and Project Personnel to the Launch Control Center (LCC). This task involved me using a Microsoft Office data processing tool to assist with the analysis and information management of logistics worth millions of dollars. With the assistance of two other interns, I was responsible for collecting data on equipment used, on a daily basis, by over 200 KSC employees. The many network servers, enterprise switches, desktop computers, and fiber optics had to be handled in an equally prompt and precise manner in order to ensure a minimal amount of equipment down time; which is critical in ensuring a properly secured networking environment. The second part of my project was to assist KSC in developing a more cost effective way of maintaining and taking full advantage of the functionality of some new kiosk units. Since KSC currently has no expert on the servicing and maintenance of the units, I, as a computer technology major, was given the opportunity to assess the hardware and software of the machines. The goal was to learn to establish a secure and remote environment for the kiosks; a goal highly valuing convenience by preserving valuable man-hours saved by not having to travel to each individual kiosk location. In addition, I was to leave a clear and precise plan for future users and administrators of the devices to follow.

Equivalent Mass versus Life Cycle Cost for Life Support Technology Selection

NASA Technical Reports Server (NTRS)

Jones, Harry

2003-01-01

The decision to develop a particular life support technology or to select it for flight usually depends on the cost to develop and fly it. Other criteria such as performance, safety, reliability, crew time, and technical and schedule risk are considered, but cost is always an important factor. Because launch cost would account for much of the cost of a future planetary mission, and because launch cost is directly proportional to the mass launched, equivalent mass has been used instead of cost to select advanced life support technology. The equivalent mass of a life support system includes the estimated mass of the hardware and of the spacecraft pressurized volume, power supply, and cooling system that the hardware requires. The equivalent mass of a system is defined as the total payload launch mass needed to provide and support the system. An extension of equivalent mass, Equivalent System Mass (ESM), has been established for use in the Advanced Life Support project. ESM adds a mass-equivalent of crew time and possibly other cost factors to equivalent mass. Traditional equivalent mass is strictly based on flown mass and reflects only the launch cost. ESM includes other important cost factors, but it complicates the simple flown mass definition of equivalent mass by adding a non-physical mass penalty for crew time that may exceed the actual flown mass. Equivalent mass is used only in life support analysis. Life Cycle Cost (LCC) is much more commonly used. LCC includes DDT&E, launch, and operations costs. For Earth orbit rather than planetary missions, the launch cost is less than the cost of Design, Development, Test, and Evaluation (DDTBE). LCC is a more inclusive cost estimator than equivalent mass. The relative costs of development, launch, and operations vary depending on the mission destination and duration. Since DDTBE or operations may cost more than launch, LCC gives a more accurate relative cost ranking than equivalent mass. To select the lowest cost technology for a particular application we should use LCC rather than equivalent mass.

Land-Use and Climate : first results from the LUCID experiments ; implications for experimental design in IPCC-AR5

NASA Astrophysics Data System (ADS)

de Noblet, N.; Pitman, A.; Participants, Lucid

2009-04-01

The project "Land-Use and Climate, IDentification of robust impacts" (LUCID) was conceived under the auspices of IGBP-iLEAPS and GEWEX-GLASS, to address the robustness of 'local' and possible remote impacts of land-use induced land-cover changes (LCC). LUCID explores, using methodologies that major climate modelling groups recognise, those impacts of LCC that are robust - that is, above the noise generated by model variability and consistent across a suite of climate models. To start with, seven climate models were run, in ensemble mode (5 realisations per 31-years long experiment), with prescribed observed sea-surface temperatures (SSTs) and sea ice extent (SIc). Pre-industrial and present-day simulations were used to explore the impacts of biogeophysical impacts of human-induced land cover change. The imposed LCC perturbation led to statistically significant changes in latent heat flux and near-surface temperature over the regions of land cover change, but few significant changes in precipitation. Our results show no common remote impacts of land cover change. They also highlight a dilemma for both historical hind-casts and future projections; land cover change is regionally important, but it is not feasible within the time frame of the next IPCC (AR5) assessment to implement this change commonly across multiple models. Further analysis are in progress and will be presented to identify the continental regions where changes in LCC may have been more important than the combined changes in SSTs, SIc and CO2 between the pre-industrial times and nowadays.

Tobacco industry use of flavours to recruit new users of little cigars and cigarillos.

PubMed

Kostygina, Ganna; Glantz, Stanton A; Ling, Pamela M

2016-01-01

While flavoured cigarettes were prohibited in the USA in 2009, flavoured little cigars and cigarillos (LCCs) remain on the market. We describe the evolving strategies used by tobacco companies to encourage uptake of flavoured LCCs and industry research findings on consumer perceptions of flavoured LCC products. Analysis of internal tobacco industry documents was triangulated with data from tobacco advertisement archives, national newspapers, trade press and the internet. Flavoured LCC products were associated with young and inexperienced tobacco users, women and African-Americans. Internal industry studies confirmed that menthol and candy-like flavours (eg, vanilla and cherry) increased LCC appeal to starters by masking the heavy cigar taste, reducing throat irritation and making LCC smoke easier to inhale. To appeal to new users, manufacturers also reduced the size of cigars to make them more cigarette-like, introduced filters and flavoured filter tips, emphasised mildness and ease of draw in advertising, and featured actors using little cigars in television commercials. RJ Reynolds tried to capitalise on the popularity of menthol cigarettes among African-Americans and marketed a menthol little cigar to African-Americans. Tobacco companies engaged in a calculated effort to blur the line between LCCs to increase the appeal to cigarette smokers, and the use of flavours facilitated these efforts. Bans on flavoured cigarettes should be expanded to include flavoured LCCs, and tobacco use prevention initiatives should include LCCs. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/

Optimizing conceptual aircraft designs for minimum life cycle cost

NASA Technical Reports Server (NTRS)

Johnson, Vicki S.

1989-01-01

A life cycle cost (LCC) module has been added to the FLight Optimization System (FLOPS), allowing the additional optimization variables of life cycle cost, direct operating cost, and acquisition cost. Extensive use of the methodology on short-, medium-, and medium-to-long range aircraft has demonstrated that the system works well. Results from the study show that optimization parameter has a definite effect on the aircraft, and that optimizing an aircraft for minimum LCC results in a different airplane than when optimizing for minimum take-off gross weight (TOGW), fuel burned, direct operation cost (DOC), or acquisition cost. Additionally, the economic assumptions can have a strong impact on the configurations optimized for minimum LCC or DOC. Also, results show that advanced technology can be worthwhile, even if it results in higher manufacturing and operating costs. Examining the number of engines a configuration should have demonstrated a real payoff of including life cycle cost in the conceptual design process: the minimum TOGW of fuel aircraft did not always have the lowest life cycle cost when considering the number of engines.

The Effect of Water Molecules on Mechanical Properties of Bamboo Microfibrils

NASA Astrophysics Data System (ADS)

Rahbar, Nima

Bamboo fibers have higher strength-to-weight ratios than steel and concrete. The unique properties of bamboo fibers come from their natural composite structures that comprise mainly cellulose nanofibrils in a matrix of intertwined hemicellulose and lignin called lignin-carbohydrate complex (LCC). Here, we have utilized atomistic simulations to investigate the mechanical properties and mechanisms of interactions between these materials, in the presence of water molecules. Our results suggest that hemicellulose exhibits better mechanical properties and lignin shows greater tendency to adhere to cellulose nanofibrils. Consequently, the role of hemicellulose found to be enhancing the mechanical properties and lignin found to be providing the strength of bamboo fibers. The abundance of Hbonds in hemicellulose chains is responsible for improving the mechanical behavior of LCC. The strong van der Waals forces between lignin molecules and cellulose nanofibrils is responsible for higher adhesion energy between LCC/cellulose nanofibrils. We also found out that the amorphous regions of cellulose nanofibrils is the weakest interface in bamboo Microfibrils. In presence of water, the elastic modulus of lignin increases at low water content (less than 10 NSF CAREER Grant No. 1261284.

Mapping Climate Science Information Needs and Networks in the Northwest, USA through Evaluating the Northwest Climate Science Center Climate Science Digest

NASA Astrophysics Data System (ADS)

Gergel, D. R.; Watts, L. H.; Salathe, E. P.; Mankowski, J. D.

2017-12-01

Climate science, already a highly interdisciplinary field, is rapidly evolving, and natural resource managers are increasingly involved in policymaking and adaptation decisions to address climate change that need to be informed by state-of-the-art climate science. Consequently, there is a strong demand for unique organizations that engender collaboration and cooperation between government, non-profit, academic and for-profit sectors that are addressing issues relating to natural resources management and climate adaptation and resilience. These organizations are often referred to as boundary organizations. The Northwest Climate Science Center (NW CSC) and the North Pacific Landscape Conservation Cooperative (NP LCC) are two such boundary organizations operating in different contexts. Together, the NW CSC and the NP LCC fulfill the need for sites of co-production between researchers and managers working on climate-related issues, and a key component of this work is a monthly climate science newsletter that includes recent climate science journal articles, reports, and climate-related events. Our study evaluates the effectiveness of the climate science digest (CSD) through a three-pronged approach: a) in-depth interviews with natural resource managers who use the CSD, b) poll questions distributed to CSD subscribers, and c) quantitative analysis of CSD effectiveness using analytics from MailChimp distribution. We aim to a) map the reach of the CSD across the Northwest and at a national level; b) understand the efficacy of the CSD at communicating climate science to diverse audiences; c) evaluate the usefulness of CSD content for diverse constituencies of subscribers; d) glean transferrable knowledge for future evaluations of boundary management tools; and e) establish a protocol for designing climate science newsletters for other agencies disseminating climate science information. We will present results from all three steps of our evaluation process and describe their implications for future evaluations of climate science communications products and other boundary management tools in the field of natural resources management.

Robustness of airline route networks

NASA Astrophysics Data System (ADS)

Lordan, Oriol; Sallan, Jose M.; Escorihuela, Nuria; Gonzalez-Prieto, David

2016-03-01

Airlines shape their route network by defining their routes through supply and demand considerations, paying little attention to network performance indicators, such as network robustness. However, the collapse of an airline network can produce high financial costs for the airline and all its geographical area of influence. The aim of this study is to analyze the topology and robustness of the network route of airlines following Low Cost Carriers (LCCs) and Full Service Carriers (FSCs) business models. Results show that FSC hubs are more central than LCC bases in their route network. As a result, LCC route networks are more robust than FSC networks.

Main propulsion system design recommendations for an advanced Orbit Transfer Vehicle

NASA Technical Reports Server (NTRS)

Redd, L.

1985-01-01

Various main propulsion system configurations of an advanced OTV are evaluated with respect to the probability of nonindependent failures, i.e., engine failures that disable the entire main propulsion system. Analysis of the life-cycle cost (LCC) indicates that LCC is sensitive to the main propulsion system reliability, vehicle dry weight, and propellant cost; it is relatively insensitive to the number of missions/overhaul, failures per mission, and EVA and IVA cost. In conclusion, two or three engines are recommended in view of their highest reliability, minimum life-cycle cost, and fail operational/fail safe capability.

Early Cretaceous adakitic magmatism in central eastern China controlled by ridge subduction

NASA Astrophysics Data System (ADS)

Ling, M.; Luo, Z.; Sun, W.

2017-12-01

Early Cretaceous adakites are widely distributed in central eastern China, e.g., Lower Yangtze River Belt (LYRB), Dabie orogen and south Tan-Lu Fault (STLF) area. Adakite from the LYRB is closely associated with mineralization, while adakites from Dabie orogen and STLF are ore barren. Their origins, however, remain controversial. Detailed geochemical comparison between these adakites indicates that the LYRB adakite are formed by partial melting of oceanic crust, i.e., slab melting, whereas those from Dabie orogen and STLF (e.g., Guandian pluton) have origin of lower continental crust (LCC) 1,2. Base on the distribution of igneous rocks, e.g., adakite, A-type granite and Nb-enriched basalts, as well as other lines of evidence, ridge subduction of the Pacific and Izanagi plates was proposed to explain the genesis of Cretaceous magmatism and associated mineralization in the LYRB 1. Ridge subduction is a special plate tectonic process that can provide both physical erosion and thermal erosion 3. Flat subduction of a spreading ridge will result in strong physical subduction-related erosion, and trigger destruction (e.g., in the Dabie orogen) or delamination (e.g., in the STLF) of the thickened LCC. Subsequently, ridge subduction, accompanied by opening of a slab window, will facilitate partial melting of the LCC by thermal erosion. References: 1. Ling, M. X. et al. Cretaceous ridge subduction along the Lower Yangtze river belt, eastern China. Econ. Geol. 104, 303-321, doi:10.2113/gsecongeo.104.2.303 (2009). 2. Ling, M. X., Wang, F. Y., Ding, X., Zhou, J. B. & Sun, W. D. Different origins of adakites from the Dabie Mountains and the Lower Yangtze River Belt, eastern China: Geochemical constraints. International Geology Review 53, 727-740 (2011). 3. Ling, M. X. et al. Destruction of the North China Craton Induced by Ridge Subductions. Journal of Geology 121, 197-213 (2013).

Origins location of the outflow tract ventricular arrhythmias exhibiting qrS pattern or QS pattern with a notch on the descending limb in lead V1.

PubMed

Lin, Cong; Zheng, Cheng; Zhou, De-Pu; Li, Xiao-Wei; Wu, Shu-Jie; Lin, Jia-Feng

2017-05-15

Ventricular outflow tract(VOT) ventricular arrhythmias(VAs) presenting qrS pattern or QS pattern with a notch on the descending limb in lead V1 were consistently thought of arising from the commissure between left and right coronary cusp (L-RCC) by previous studies. However, we found they could originate from other anatomic structures in VOT. This study aimed to investigate the exact origin of this kind VAs. Forty-nine patients of VOT premature ventricular contrations/ventricular tachycardia(PVCs/VT) with lead V1 presenting qrS pattern or QS pattern with a notch on the descending limb undergoing successful radiofrequency catheter ablation(RFCA) in our center were analyzed. 12-lead electrocardiogram(ECG) of these PVCs/VT were summarized. Among these PVCs/VT, 37 cases exhibited qrS morphology in lead V1, 12 cases presented QS pattern with a notch on the descending limb in the same lead. Based on the successful ablation sites, these PVCs/VT were divided into 2 groups: (1)Right ventricular outflow tract(RVOT) group (26 cases), and (2) Left ventricular outflow tract (LVOT) group(23 cases, 4 cases originating from the left coronary cusp(LCC), 2 from the right coronary cusp(RCC), 16 from the L-RCC, 1 from the area inferior to LCC(ILCC)). The ECG characteristics of each PVCs/VT were analyzed. Among these PVCs/VT, applying the precordial transitional zone index(TZ index) < 0 to predict LVOT origin was demonstrated with sensitivity of 95.65%, specificity of 96.15%, positive predicting value(PPV) of 95.65% and negative predicting value(NPV) of 96.15%. In LVOT group, further applying the r, R, m,or Rs morphology in lead I to predict L-RCC and RCC origin was demonstrated with sensitivity of 94.44%, specificity of 60.00%, PPV of 89.47% and NPV of 75.00%. Ventricular outflow tract PVCs/VT with lead V1 presenting qrS pattern or QS pattern with a notch on descending limb not only arising from L-RCC, but also RVOT, LCC, RCC and ILCC. Combining TZ index and QRS morphology in lead I to predict origin site of these kind VAs is a convenient, simple and reliable method and facilitates the RFCA procedure.

Preliminary testing of flow-ecology hypotheses developed for the GCP LCC region

USGS Publications Warehouse

Brewer, Shannon K.; Davis, Mary

2014-01-01

The Ecological Limits of Hydrological Alteration (ELOHA) framework calls for the development of flow-ecology hypotheses to support protection of the flow regime from ecologically harmful alteration due to human activities. As part of a larger instream flow project for the Gulf Coast Prairie Landscape Conservation Cooperative (GCP LCC), regional flow-ecology hypotheses were developed for fish, mussels, birds, and riparian vegetation (Davis and Brewer 20141). The objective of this study was to assess the usefulness of existing ecological and hydrological data to test these hypotheses or others that may be developed in the future. Several databases related to biological collections and hydrologic data from Oklahoma, Texas, and Louisiana were compiled. State fish-community data from Oklahoma and Louisiana were summarized and paired with existing USGS gage data having at least a 40-year period of record that could be separated into reference and current conditions for comparison. The objective of this study was not to conduct exhaustive analyses of these data, the hypotheses, or analyses interpretation, but rather to use these data to determine if existing data were adequate to statistically test the regional flow-ecology hypotheses. The regional flow-ecology hypotheses were developed for the GCP LCC by a committee chaired by Shannon Brewer and Mary Davis (Davis and Brewer 2014). Existing data were useful for informing the hypotheses and suggest support for some hypotheses, but also highlight the need for additional testing and development as some results contradicted hypotheses. Results presented here suggest existing data are adequate to support some flow-ecology hypotheses; however, lack of sampling effort reported with the fish collections and the need for ecoregion-specific analyses suggest more data would be beneficial to analyses in some ecoregions. Additional fish sampling data from Texas and Louisiana will be available for future analyses and may ameliorate some of the data concerns and improve hypothesis interpretation. If the regional hydrologic model currently under development by the U.S. Geological Survey for the South-Central Climate Science Center is improved to produce daily hydrographs, it will enable use of fish data at ungaged locations. In future efforts, exhaustive analyses using these data, in addition to the development of more complex multivariate hypotheses, would be beneficial to understanding data gaps, particularly as relevant to species of conservation concern.

Comparative study of lignin characteristics from wheat straw obtained by soda-AQ and kraft pretreatment and effect on the following enzymatic hydrolysis process

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yang, Haitao; Xie, Yimin; Zheng, Xing

With this study, to understand the structural changes of lignin after soda-AQ and kraft pretreatment, milled straw lignin, black liquor lignin and residual lignin extracted from wheat straw were characterized by FT-IR, UV, GPC and NMR. The results showed that the main lignin linkages were β-aryl ether substructures (β-O-4'), followed by phenylcoumaran (β-5') and resinol (β-β') substructures, while minor content of spirodienone (β-1'), dibenzodioxocin (5-5') and α,β-diaryl ether linkages were detected as well. After pretreatment, most lignin inter-units and lignin-carbohydrate complex (LCC) linkages were degraded and dissolved in black liquor, with minor amount left in residual pretreated biomass. In addition,more » through quantitative 13C and 2D-HSQC NMR spectral analysis, lignin and LCC were found to be more degraded after kraft pretreatment than soda-AQ pretreatment. Furthermore, the subsequent enzymatic hydrolysis results showed that more cellulose in wheat straw was converted to glucose after kraft pretreatment, indicating that LCC linkages were important in the enzymatic hydrolysis process.« less

COUP-TFII inhibits NFkappaB activation in endocrine-resistant breast cancer cells

PubMed Central

Litchfield, Lacey M.; Appana, Savitri N.; Datta, Susmita; Klinge, Carolyn M.

2016-01-01

Reduced COUP-TFII expression contributes to endocrine resistance in breast cancer cells. Endocrine-resistant breast cancer cells have higher NFkappa B (NFκB) activity and target gene expression. The goal of this study was to determine if COUP-TFII modulates NFκB activity. Endocrine-resistant LCC9 cells with low endogenous COUP-TFII displayed ~5-fold higher basal NFκB activity than parental endocrine-sensitive MCF-7 breast cancer cells. Transient transfection of LCC9 cells with COUP-TFII inhibited NFκB activation and reduced NFκB target gene expression. COUP-TFII and NFκB were inversely correlated in breast cancer patient samples. Endogenous COUP-TFII coimmunoprecipitated with NFκB subunits RelB and NFκB1 in MCF-7 cells. COUP-TFII inhibited NFκB-DNA binding in vitro and impaired coactivator induced NFκB transactivation. LCC9 cells were growth-inhibited by an NFκB inhibitor and 4-hydroxytamoxifen compared to MCF-7 cells. Together these data indicate a novel role for COUP-TFII in suppression of NFκB activity and explain, in part, why decreased COUP-TFII expression results in an endocrine-resistant phenotype. PMID:24141032

Comparative study of lignin characteristics from wheat straw obtained by soda-AQ and kraft pretreatment and effect on the following enzymatic hydrolysis process

DOE PAGES

Yang, Haitao; Xie, Yimin; Zheng, Xing; ...

2016-02-18

With this study, to understand the structural changes of lignin after soda-AQ and kraft pretreatment, milled straw lignin, black liquor lignin and residual lignin extracted from wheat straw were characterized by FT-IR, UV, GPC and NMR. The results showed that the main lignin linkages were β-aryl ether substructures (β-O-4'), followed by phenylcoumaran (β-5') and resinol (β-β') substructures, while minor content of spirodienone (β-1'), dibenzodioxocin (5-5') and α,β-diaryl ether linkages were detected as well. After pretreatment, most lignin inter-units and lignin-carbohydrate complex (LCC) linkages were degraded and dissolved in black liquor, with minor amount left in residual pretreated biomass. In addition,more » through quantitative 13C and 2D-HSQC NMR spectral analysis, lignin and LCC were found to be more degraded after kraft pretreatment than soda-AQ pretreatment. Furthermore, the subsequent enzymatic hydrolysis results showed that more cellulose in wheat straw was converted to glucose after kraft pretreatment, indicating that LCC linkages were important in the enzymatic hydrolysis process.« less

Life cycle costing of waste management systems: overview, calculation principles and case studies.

PubMed

Martinez-Sanchez, Veronica; Kromann, Mikkel A; Astrup, Thomas Fruergaard

2015-02-01

This paper provides a detailed and comprehensive cost model for the economic assessment of solid waste management systems. The model was based on the principles of Life Cycle Costing (LCC) and followed a bottom-up calculation approach providing detailed cost items for all key technologies within modern waste systems. All technologies were defined per tonne of waste input, and each cost item within a technology was characterised by both a technical and an economic parameter (for example amount and cost of fuel related to waste collection), to ensure transparency, applicability and reproducibility. Cost items were classified as: (1) budget costs, (2) transfers (for example taxes, subsidies and fees) and (3) externality costs (for example damage or abatement costs related to emissions and disamenities). Technology costs were obtained as the sum of all cost items (of the same type) within a specific technology, while scenario costs were the sum of all technologies involved in a scenario. The cost model allows for the completion of three types of LCC: a Conventional LCC, for the assessment of financial costs, an Environmental LCC, for the assessment of financial costs whose results are complemented by a Life Cycle Assessment (LCA) for the same system, and a Societal LCC, for socio-economic assessments. Conventional and Environmental LCCs includes budget costs and transfers, while Societal LCCs includes budget and externality costs. Critical aspects were found in the existing literature regarding the cost assessment of waste management, namely system boundary equivalency, accounting for temporally distributed emissions and impacts, inclusions of transfers, the internalisation of environmental impacts and the coverage of shadow prices, and there was also significant confusion regarding terminology. The presented cost model was implemented in two case study scenarios assessing the costs involved in the source segregation of organic waste from 100,000 Danish households and the subsequent co-digestion of organic waste with animal manure. Overall, source segregation resulted in higher financial costs than the alternative of incinerating the organic waste with the residual waste: 1.6 M€/year, of which 0.9 M€/year was costs for extra bins and bags used by the households, 1.0 M€/year for extra collections and -0.3 M€/year saved on incineration. Copyright © 2014 Elsevier Ltd. All rights reserved.

Investigating the Potential of Activity Tracking App Data to Estimate Cycle Flows in Urban Areas

NASA Astrophysics Data System (ADS)

Haworth, J.

2016-06-01

Traffic congestion and its associated environmental effects pose a significant problem for large cities. Consequently, promoting and investing in green travel modes such as cycling is high on the agenda for many transport authorities. In order to target investment in cycling infrastructure and improve the experience of cyclists on the road, it is important to know where they are. Unfortunately, investment in intelligent transportation systems over the years has mainly focussed on monitoring vehicular traffic, and comparatively little is known about where cyclists are on a day to day basis. In London, for example, there are a limited number of automatic cycle counters installed on the network, which provide only part of the picture. These are supplemented by surveys that are carried out infrequently. Activity tracking apps on smart phones and GPS devices such as Strava have become very popular over recent years. Their intended use is to track physical activity and monitor training. However, many people routinely use such apps to record their daily commutes by bicycle. At the aggregate level, these data provide a potentially rich source of information about the movement and behaviour of cyclists. Before such data can be relied upon, however, it is necessary to examine their representativeness and understand their potential biases. In this study, the flows obtained from Strava Metro (SM) are compared with those obtained during the 2013 London Cycle Census (LCC). A set of linear regression models are constructed to predict LCC flows using SM flows along with a number of dummy variables including road type, hour of day, day of week and presence/absence of cycle lane. Cross-validation is used to test the fitted models on unseen LCC sites. SM flows are found to be a statistically significant (p<0.0001) predictor of total flows as measured by the LCC and the models yield R squared statistics of ~0.7 before considering spatio-temporal variation. The initial results indicate that data collected using fitness tracking apps such as Strava are a promising data source for traffic managers. Future work will incorporate the spatio-temporal structure in the data to better account for the spatial and temporal variation in the ratio of SM flows to LCC flows.

Application of Life Support Cost, Provisioning, and Repair/Discard Models to Weapon System Procurement Decisions by Small Countries.

DTIC Science & Technology

1981-12-01

the LCC for use in system evaluation was motivated by the fact that the major part of user budgets are spent on operations and support activities...Furthermore, it was recognized that these ownership costs ex- ceed systems procurement costs by up to several times. The main motivation behind the LCC...8217 󈧄 4 ~ ~ ~ ( CL f %AO 09*. 𔃾.𔃺 𔄃 0 4 . -~4 4C4 - CL44 N 10 z3 w 4 . - 4)..LIP 4 .J~272 .0co A pF 1 PS4 ~~~~m a0~-eN ON~ - .q r N0 .. ~ ~ 7O N#04

Stationary variational estimates for the effective response and field fluctuations in nonlinear composites

NASA Astrophysics Data System (ADS)

Ponte Castañeda, Pedro

2016-11-01

This paper presents a variational method for estimating the effective constitutive response of composite materials with nonlinear constitutive behavior. The method is based on a stationary variational principle for the macroscopic potential in terms of the corresponding potential of a linear comparison composite (LCC) whose properties are the trial fields in the variational principle. When used in combination with estimates for the LCC that are exact to second order in the heterogeneity contrast, the resulting estimates for the nonlinear composite are also guaranteed to be exact to second-order in the contrast. In addition, the new method allows full optimization with respect to the properties of the LCC, leading to estimates that are fully stationary and exhibit no duality gaps. As a result, the effective response and field statistics of the nonlinear composite can be estimated directly from the appropriately optimized linear comparison composite. By way of illustration, the method is applied to a porous, isotropic, power-law material, and the results are found to compare favorably with earlier bounds and estimates. However, the basic ideas of the method are expected to work for broad classes of composites materials, whose effective response can be given appropriate variational representations, including more general elasto-plastic and soft hyperelastic composites and polycrystals.

Adolescent and Young Adult Perceptions of Hookah and Little Cigars/Cigarillos: Implications for Risk Messages

PubMed Central

Cornacchione, Jennifer; Wagoner, Kimberly G.; Wiseman, Kimberly D.; Kelley, Dannielle; Noar, Seth M.; Smith, Margaret H.; Sutfin, Erin L.

2016-01-01

Use of hookah and little cigars/cigarillos (LCCs) is high among adolescents and young adults. Although these products have health effects similar to cigarettes, adolescents and young adults believe them to be safer. This study examined adolescent and young adult perceptions of hookah and LCCs to develop risk messages aimed at discouraging use among users and at-risk nonusers. Ten focus groups with 77 adolescents and young adults were conducted to explore their perceptions about the perceived risks and benefits of hookah and LCC use. Participants were users of other (non-cigarette) tobacco products (n=47) and susceptible nonusers (n=30). Transcripts were coded for emergent themes on participants’ perceptions of hookah and LCCs. Participants did not perceive health effects associated with hookah and LCC use to be serious or likely to happen given their infrequency of use and perceptions that they are less harmful than cigarettes. Participants generally had positive associations with smoking hookah and LCCs for several reasons, including that they are used in social gatherings, come in various flavors, and can be used to perform smoke tricks. Because adolescents and young adults underestimate and discount the long-term risks associated with hookah and LCC use, effective messages may be those that focus on the acute/immediate health and cosmetic effects. PMID:27337629

The Effect of Water Molecules on Mechanical Properties of Cell Walls

NASA Astrophysics Data System (ADS)

Rahbar, Nima; Youssefian, Sina

The unique properties of bamboo fibers come from their natural composite structures that comprise mainly cellulose nanofibrils in a matrix of intertwined hemicellulose and lignin called lignin-carbohydrate complex (LCC). Here, we have utilized atomistic simulations to investigate the mechanical properties and mechanisms of interactions between these materials, in the presence of water molecules. The role of hemicellulose found to be enhancing the mechanical properties and lignin found to be providing the strength of bamboo fibers. The abundance of Hbonds in hemicellulose chains is responsible for improving the mechanical behavior of LCC. The strong van der Waals forces between lignin molecules and cellulose nanofibrils are responsible for higher adhesion energy between LCC/cellulose nanofibrils. We also found out that the amorphous regions of cellulose nanofibrils is the weakest interface in bamboo Microfibrils. In presence of water, the elastic modulus of lignin increases at low water content and decreases in higher water content, whereas the hemicellulose elastic modulus constantly decreases. The variations of Radial Distribution Function and Free Fractional Volume of these materials with water suggest that water molecules enhance the mechanical properties of lignin by filling voids in the system and creating Hbond bridges between polymer chains. For hemicellulose, however, the effect is always regressive due to the destructive effect of water molecules on the Hbond of its dense structure.

The variation of cloud amount and light rainy days under heavy pollution over South China during 1960-2009.

PubMed

Fu, Chuanbo; Dan, Li

2018-01-01

The ground observation data was used to analyze the variation of cloud amount and light precipitation over South China during 1960-2009. The total cloud cover (TCC) decreases in this period, whereas the low cloud cover (LCC) shows the obvious opposite change with increasing trends. LCP defined as low cloud cover/total cloud cover has increased, and small rainy days (< 10 mm day -1 ) decreased significantly (passing 0.001 significance level) during the past 50 years, which is attributed to the enhanced levels of air pollution in the form of anthropogenic aerosols. The horizontal visibility and sunshine duration are used to depict the anthropogenic aerosol loading. When horizontal visibility declines to 20 km or sunshine duration decreases to 5 h per day, LCC increases 52% or more and LCP increases significantly. The correlation coefficients between LCC and horizontal visibility or sunshine duration are - 0.533 and - 0.927, and the values between LCP and horizontal visibility or sunshine duration are - 0.849 and - 0.641, which pass 0.001 significance level. The results indicated that aerosols likely impacted the long-term trend of cloud amount and light precipitation over South China.

Web-based automation of green building rating index and life cycle cost analysis

NASA Astrophysics Data System (ADS)

Shahzaib Khan, Jam; Zakaria, Rozana; Aminuddin, Eeydzah; IzieAdiana Abidin, Nur; Sahamir, Shaza Rina; Ahmad, Rosli; Nafis Abas, Darul

2018-04-01

Sudden decline in financial markets and economic meltdown has slow down adaptation and lowered interest of investors towards green certified buildings due to their higher initial costs. Similarly, it is essential to fetch investor’s attention towards more development of green buildings through automated tools for the construction projects. Though, historical dearth is found on the automation of green building rating tools that brings up an essential gap to develop an automated analog computerized programming tool. This paper present a proposed research aim to develop an integrated web-based automated analog computerized programming that applies green building rating assessment tool, green technology and life cycle cost analysis. It also emphasizes to identify variables of MyCrest and LCC to be integrated and developed in a framework then transformed into automated analog computerized programming. A mix methodology of qualitative and quantitative survey and its development portray the planned to carry MyCrest-LCC integration to an automated level. In this study, the preliminary literature review enriches better understanding of Green Building Rating Tools (GBRT) integration to LCC. The outcome of this research is a pave way for future researchers to integrate other efficient tool and parameters that contributes towards green buildings and future agendas.

LCC demons with divergence term for liver MRI motion correction

NASA Astrophysics Data System (ADS)

Oh, Jihun; Martin, Diego; Skrinjar, Oskar

2010-03-01

Contrast-enhanced liver MR image sequences acquired at multiple times before and after contrast administration have been shown to be critically important for the diagnosis and monitoring of liver tumors and may be used for the quantification of liver inflammation and fibrosis. However, over multiple acquisitions, the liver moves and deforms due to patient and respiratory motion. In order to analyze contrast agent uptake one first needs to correct for liver motion. In this paper we present a method for the motion correction of dynamic contrastenhanced liver MR images. For this purpose we use a modified version of the Local Correlation Coefficient (LCC) Demons non-rigid registration method. Since the liver is nearly incompressible its displacement field has small divergence. For this reason we add a divergence term to the energy that is minimized in the LCC Demons method. We applied the method to four sequences of contrast-enhanced liver MR images. Each sequence had a pre-contrast scan and seven post-contrast scans. For each post-contrast scan we corrected for the liver motion relative to the pre-contrast scan. Quantitative evaluation showed that the proposed method improved the liver alignment relative to the non-corrected and translation-corrected scans and visual inspection showed no visible misalignment of the motion corrected contrast-enhanced scans and pre-contrast scan.

The Influences of Drought and Land-Cover Conversion on Inter-Annual Variation of NPP in the Three-North Shelterbelt Program Zone of China Based on MODIS Data

PubMed Central

Wu, Chaoyang; Zhang, Bing; Huete, Alfredo; Zhang, Xiaoyang; Sun, Rui; Lei, Liping; Huang, Wenjing; Liu, Liangyun; Liu, Xinjie; Li, Jun; Luo, Shezhou; Fang, Bin

2016-01-01

Terrestrial ecosystems greatly contribute to carbon (C) emission reduction targets through photosynthetic C uptake.Net primary production (NPP) represents the amount of atmospheric C fixed by plants and accumulated as biomass. The Three-North Shelterbelt Program (TNSP) zone accounts for more than 40% of China’s landmass. This zone has been the scene of several large-scale ecological restoration efforts since the late 1990s, and has witnessed significant changes in climate and human activities.Assessing the relative roles of different causal factors on NPP variability in TNSP zone is very important for establishing reasonable local policies to realize the emission reduction targets for central government. In this study, we examined the relative roles of drought and land cover conversion(LCC) on inter-annual changes of TNSP zone for 2001–2010. We applied integrated correlation and decomposition analyses to a Standardized Evapotranspiration Index (SPEI) and MODIS land cover dataset. Our results show that the 10-year average NPP within this region was about 420 Tg C. We found that about 60% of total annual NPP over the study area was significantly correlated with SPEI (p<0.05). The LCC-NPP relationship, which is especially evident for forests in the south-central area, indicates that ecological programs have a positive impact on C sequestration in the TNSP zone. Decomposition analysis generally indicated that the contributions of LCC, drought, and other Natural or Anthropogenic activities (ONA) to changes in NPP generally had a consistent distribution pattern for consecutive years. Drought and ONA contributed about 74% and 23% to the total changes in NPP, respectively, and the remaining 3% was attributed to LCC. Our results highlight the importance of rainfall supply on NPP variability in the TNSP zone. PMID:27348303

Downcycling versus recycling of construction and demolition waste: Combining LCA and LCC to support sustainable policy making.

PubMed

Di Maria, Andrea; Eyckmans, Johan; Van Acker, Karel

2018-05-01

Urgent solutions are needed in Europe to deal with construction and demolition waste (CDW). EU policy has contributed to significantly reducing the amount of CDW going to landfill, but most of the effort has been put in downcycling practices. Therefore, further policies are needed to stimulate high-quality recycling of CDW. The present paper presents a combined life cycle assessment (LCA) and life cycle costing (LCC) methodologies to analyse the environmental and the economic drivers in four alternative CDW end-of-life scenarios in the region of Flanders, in Belgium. The four analysed alternatives are (i) landfilling, (ii) downcycling, (iii) advanced recycling and (iv) recycling after selective demolition. LCA results show that landiflling is the scenario having the highest environmental impacts in terms of person equivalent (PE), followed by downcycling and recycling (-36%) and recycling after selective demolition (-59%). The decrease in environmental impacts is mostly due to the avoided landfilling of CDW and the recovery of materials from selective demolition. LCC results indicate that landfilling is the scenario bearing the highest total economic costs. This is due to the high landfill tax in Flanders. The recycling after selective demolition bears the second highest cost. The increase of high-quality CDW recycling can significantly reduce the overall environmental impact of the system. Implementing a high landfill tax, increasing the gate fee to the recycling plant, and boosting the sales price of recycled aggregates are the most effective drivers to facilitate a transition towards a more sustainable CDW management system. The paper demonstrates that the combined LCA and LCC results can highlight the environmental and economic drivers in CDW management. The results of the combined analysis can help policymakers to promote the aspects contributing to sustainability and to limit the ones creating a barrier. Copyright © 2018 Elsevier Ltd. All rights reserved.

Improved estimation of leaf area index and leaf chlorophyll content of a potato crop using multi-angle spectral data - potential of unmanned aerial vehicle imagery

NASA Astrophysics Data System (ADS)

Roosjen, Peter P. J.; Brede, Benjamin; Suomalainen, Juha M.; Bartholomeus, Harm M.; Kooistra, Lammert; Clevers, Jan G. P. W.

2018-04-01

In addition to single-angle reflectance data, multi-angular observations can be used as an additional information source for the retrieval of properties of an observed target surface. In this paper, we studied the potential of multi-angular reflectance data for the improvement of leaf area index (LAI) and leaf chlorophyll content (LCC) estimation by numerical inversion of the PROSAIL model. The potential for improvement of LAI and LCC was evaluated for both measured data and simulated data. The measured data was collected on 19 July 2016 by a frame-camera mounted on an unmanned aerial vehicle (UAV) over a potato field, where eight experimental plots of 30 × 30 m were designed with different fertilization levels. Dozens of viewing angles, covering the hemisphere up to around 30° from nadir, were obtained by a large forward and sideways overlap of collected images. Simultaneously to the UAV flight, in situ measurements of LAI and LCC were performed. Inversion of the PROSAIL model was done based on nadir data and based on multi-angular data collected by the UAV. Inversion based on the multi-angular data performed slightly better than inversion based on nadir data, indicated by the decrease in RMSE from 0.70 to 0.65 m2/m2 for the estimation of LAI, and from 17.35 to 17.29 μg/cm2 for the estimation of LCC, when nadir data were used and when multi-angular data were used, respectively. In addition to inversions based on measured data, we simulated several datasets at different multi-angular configurations and compared the accuracy of the inversions of these datasets with the inversion based on data simulated at nadir position. In general, the results based on simulated (synthetic) data indicated that when more viewing angles, more well distributed viewing angles, and viewing angles up to larger zenith angles were available for inversion, the most accurate estimations were obtained. Interestingly, when using spectra simulated at multi-angular sampling configurations as were captured by the UAV platform (view zenith angles up to 30°), already a huge improvement could be obtained when compared to solely using spectra simulated at nadir position. The results of this study show that the estimation of LAI and LCC by numerical inversion of the PROSAIL model can be improved when multi-angular observations are introduced. However, for the potato crop, PROSAIL inversion for measured data only showed moderate accuracy and slight improvements.

Development of LC-13C NMR

NASA Technical Reports Server (NTRS)

Dorn, H. C.; Wang, J. S.; Glass, T. E.

1986-01-01

This study involves the development of C-13 nuclear resonance as an on-line detector for liquid chromatography (LC-C-13 NMR) for the chemical characterization of aviation fuels. The initial focus of this study was the development of a high sensitivity flow C-13 NMR probe. Since C-13 NMR sensitivity is of paramount concern, considerable effort during the first year was directed at new NMR probe designs. In particular, various toroid coil designs were examined. In addition, corresponding shim coils for correcting the main magnetic field (B sub 0) homogeneity were examined. Based on these initial probe design studies, an LC-C-13 NMR probe was built and flow C-13 NMR data was obtained for a limited number of samples.

Cost Structure and Life Cycle Cost (LCC) for Military Systems (structures de couts globaux de possession (LCC) pour systemes militaires)

DTIC Science & Technology

2003-06-01

variables. Dans le plan (p = 2), la méthode de régression linéaire ajuste au nuage de points une droite qui minimise la somme des écarts au carré entre...cohérence de la définition du produit. Par exemple, pour un blindé, il faut valider l ’ « harmonie » du trio masse, puissance du moteur et vitesse maximale. Il...en condition opérationnelle. De façon plus générale, la DGA devrait s’orienter vers l ‘élaboration et l’acquisition de modèle d’estimation de coûts du

Small Engine Component Technology (SECT) study

NASA Technical Reports Server (NTRS)

Singh, B.

1986-01-01

Small advanced (450 to 850 pounds thrust, 2002 to 3781 N) gas turbine engines were studied for a subsonic strategic cruise missile application, using projected year 2000 technology. An aircraft, mission characteristics, and baseline (state-of-the-art) engine were defined to evaluate technology benefits. Engine performance and configuration analyses were performed for two and three spool turbofan and propfan engine concepts. Mission and Life Cycle Cost (LCC) analyses were performed in which the candidate engines were compared to the baseline engines over a prescribed mission. The advanced technology engines reduced system LCC up to 41 percent relative to the baseline engine. Critical aerodynamic, materials, and mechanical systems turbine engine technologies were identified and program plans were defined for each identified critical technology.

Network immunization under limited budget using graph spectra

NASA Astrophysics Data System (ADS)

Zahedi, R.; Khansari, M.

2016-03-01

In this paper, we propose a new algorithm that minimizes the worst expected growth of an epidemic by reducing the size of the largest connected component (LCC) of the underlying contact network. The proposed algorithm is applicable to any level of available resources and, despite the greedy approaches of most immunization strategies, selects nodes simultaneously. In each iteration, the proposed method partitions the LCC into two groups. These are the best candidates for communities in that component, and the available resources are sufficient to separate them. Using Laplacian spectral partitioning, the proposed method performs community detection inference with a time complexity that rivals that of the best previous methods. Experiments show that our method outperforms targeted immunization approaches in both real and synthetic networks.

Landscape Conservation Cooperatives: Creating a Collaborative Conservation Vision in the Face of Climate Change Uncertainty

NASA Astrophysics Data System (ADS)

Athearn, N.; Schlafmann, D.

2015-12-01

The 22 Landscape Conservation Cooperatives (LCCs) form a "network of networks," each defined by the characteristics of its ecoregion and its unique community of conservation managers, practitioners, and scientists. As self-directed partnerships, LCCs are strongly influenced not only by the landscape but by the evolving cultures and values that define the multi-faceted relationships between people and place. LCCs maintain an ecologically connected network across these diverse landscapes by transcending borders and leveraging resources. Natural resource managers are challenged to make decisions in the face of multiple uncertainties, and several partners across the network have recognized that climate change is one important uncertainty that spans boundaries - both across the conservation community and beyond. The impacts of climate change across the LCC Network are likely to be as diverse as the network itself - manifesting as, for example, sea level rise, ocean acidification, loss of sea ice, and shifts in climate patterns and timing - but synergies are being leveraged within and between LCCs and national climate-focused programs to systematically address the needs of the network to support a collaborative conservation vision that addresses multiple landscape-scale stressors in the face of climate uncertainties. This vision is being achieved by leveraging the convening power of the LCCs and collaborating with DOI Climate Science Centers and others. Selected case studies will demonstrate how the network finds strength in its differences, but also reveals powerful collaborative opportunities through integrated science, shared conservation strategies, and strategic approaches for translating targeted science to conservation action. These examples exemplify past successes as well as ongoing efforts as the network continues to bring about effective application of climate science to achieve conservation outcomes across the LCC Network in an uncertain future climate.

Studying the Effects of Amazonian Land Cover Change on Glacier Mass Balance in the Tropical Andes

NASA Astrophysics Data System (ADS)

Mark, B. G.; Fernandez, A.; Gabrielli, P.; Montenegro, A.; Postigo, J.; Hellstrom, R. A.

2017-12-01

Recent research has highlighted several ongoing environmental changes occurring across Tropical South America, including Andean glacier retreat, drought, as well as changes in land-use and land-cover. As the regional climate of the area is mostly characterized by land-ocean interactions, the atmospheric convection in the Amazon, and the effect of the Andes on circulation patterns, it follows that changes in one of those regions may affect the other. Most scholars who have studied the causes of tropical glaciers' fluctuations have not analyzed the linkages with changes in the Amazon with the same attention paid to the influence of Pacific sea surface temperature. Here we study the response of glacier surface mass balance in the Cordillera Blanca, Peru (10°S), to a scenario where the Amazonian rainforest is replaced by savannas. We ran climatic simulations at 2-km spatial resolution utilizing the Weather Research and Forecasting (WRF) model considering two scenarios: (a) control (CRTL), with today's rainforest extent; and (b) land cover change (LCC), where all the rainforest was replaced by savanna. WRF output was in turn ingested into a glacier energy and mass balance (GEMB) model that we validate by reconstructing both the accumulated mass balance from available observations, and the altitudinal distribution of mass balance in the region. Seasonal comparison between CRTL and LCC scenarios indicates that forest replacement by savanna results in more positive glacier mass balance. This shift to more positive mass balance contrasts with a (WRF) modeled rise in the elevation of the freezing line (0°C) between 30 to 120 m for the LCC scenario. Our results are surprising because most previous studies have shown that reducing Amazon forest cover diminishes rainfall and increases temperature, suggesting that glaciers should lose mass. We hypothesize and discuss implications of possible land-atmospheric processes that might drive this tropical glacier response to Amazonian forest change, including: the large-scale influence of Amazonian albedo change on the interaction between the Walker and Hadley cells and the effect of mountain meteorology dynamics.

Computational Analysis and Low-Scale Constitutive Expression of Laccases Synthetic Genes GlLCC1 from Ganoderma lucidum and POXA 1B from Pleurotus ostreatus in Pichia pastoris

PubMed Central

Reyes-Guzmán, Edwin Alfredo; Poutou-Piñales, Raúl A.; Reyes-Montaño, Edgar Antonio; Pedroza-Rodríguez, Aura Marina; Rodríguez-Vázquez, Refugio; Cardozo-Bernal, Ángela M.

2015-01-01

Lacasses are multicopper oxidases that can catalyze aromatic and non-aromatic compounds concomitantly with reduction of molecular oxygen to water. Fungal laccases have generated a growing interest due to their biotechnological potential applications, such as lignocellulosic material delignification, biopulping and biobleaching, wastewater treatment, and transformation of toxic organic pollutants. In this work we selected fungal genes encoding for laccase enzymes GlLCC1 in Ganoderma lucidum and POXA 1B in Pleurotus ostreatus. These genes were optimized for codon use, GC content, and regions generating secondary structures. Laccase proposed computational models, and their interaction with ABTS [2, 2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)] substrate was evaluated by molecular docking. Synthetic genes were cloned under the control of Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase (GAP) constitutive promoter. P. pastoris X-33 was transformed with pGAPZαA-LaccGluc-Stop and pGAPZαA-LaccPost-Stop constructs. Optimization reduced GC content by 47 and 49% for LaccGluc-Stop and LaccPost-Stop genes, respectively. A codon adaptation index of 0.84 was obtained for both genes. 3D structure analysis using SuperPose revealed LaccGluc-Stop is similar to the laccase crystallographic structure 1GYC of Trametes versicolor. Interaction analysis of the 3D models validated through ABTS, demonstrated higher substrate affinity for LaccPost-Stop, in agreement with our experimental results with enzymatic activities of 451.08 ± 6.46 UL-1 compared to activities of 0.13 ± 0.028 UL-1 for LaccGluc-Stop. This study demonstrated that G. lucidum GlLCC1 and P. ostreatus POXA 1B gene optimization resulted in constitutive gene expression under GAP promoter and α-factor leader in P. pastoris. These are important findings in light of recombinant enzyme expression system utility for environmentally friendly designed expression systems, because of the wide range of substrates that laccases can transform. This contributes to a great gamut of products in diverse settings: industry, clinical and chemical use, and environmental applications. PMID:25611746

Computational analysis and low-scale constitutive expression of laccases synthetic genes GlLCC1 from Ganoderma lucidum and POXA 1B from Pleurotus ostreatus in Pichia pastoris.

PubMed

Rivera-Hoyos, Claudia M; Morales-Álvarez, Edwin David; Poveda-Cuevas, Sergio Alejandro; Reyes-Guzmán, Edwin Alfredo; Poutou-Piñales, Raúl A; Reyes-Montaño, Edgar Antonio; Pedroza-Rodríguez, Aura Marina; Rodríguez-Vázquez, Refugio; Cardozo-Bernal, Ángela M

2015-01-01

Lacasses are multicopper oxidases that can catalyze aromatic and non-aromatic compounds concomitantly with reduction of molecular oxygen to water. Fungal laccases have generated a growing interest due to their biotechnological potential applications, such as lignocellulosic material delignification, biopulping and biobleaching, wastewater treatment, and transformation of toxic organic pollutants. In this work we selected fungal genes encoding for laccase enzymes GlLCC1 in Ganoderma lucidum and POXA 1B in Pleurotus ostreatus. These genes were optimized for codon use, GC content, and regions generating secondary structures. Laccase proposed computational models, and their interaction with ABTS [2, 2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)] substrate was evaluated by molecular docking. Synthetic genes were cloned under the control of Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase (GAP) constitutive promoter. P. pastoris X-33 was transformed with pGAPZαA-LaccGluc-Stop and pGAPZαA-LaccPost-Stop constructs. Optimization reduced GC content by 47 and 49% for LaccGluc-Stop and LaccPost-Stop genes, respectively. A codon adaptation index of 0.84 was obtained for both genes. 3D structure analysis using SuperPose revealed LaccGluc-Stop is similar to the laccase crystallographic structure 1GYC of Trametes versicolor. Interaction analysis of the 3D models validated through ABTS, demonstrated higher substrate affinity for LaccPost-Stop, in agreement with our experimental results with enzymatic activities of 451.08 ± 6.46 UL-1 compared to activities of 0.13 ± 0.028 UL-1 for LaccGluc-Stop. This study demonstrated that G. lucidum GlLCC1 and P. ostreatus POXA 1B gene optimization resulted in constitutive gene expression under GAP promoter and α-factor leader in P. pastoris. These are important findings in light of recombinant enzyme expression system utility for environmentally friendly designed expression systems, because of the wide range of substrates that laccases can transform. This contributes to a great gamut of products in diverse settings: industry, clinical and chemical use, and environmental applications.

Three-Dimensional Cell Printing of Large-Volume Tissues: Application to Ear Regeneration.

PubMed

Lee, Jung-Seob; Kim, Byoung Soo; Seo, Donghwan; Park, Jeong Hun; Cho, Dong-Woo

2017-03-01

The three-dimensional (3D) printing of large-volume cells, printed in a clinically relevant size, is one of the most important challenges in the field of tissue engineering. However, few studies have reported the fabrication of large-volume cell-printed constructs (LCCs). To create LCCs, appropriate fabrication conditions should be established: Factors involved include fabrication time, residence time, and temperature control of the cell-laden hydrogel in the syringe to ensure high cell viability and functionality. The prolonged time required for 3D printing of LCCs can reduce cell viability and result in insufficient functionality of the construct, because the cells are exposed to a harsh environment during the printing process. In this regard, we present an advanced 3D cell-printing system composed of a clean air workstation, a humidifier, and a Peltier system, which provides a suitable printing environment for the production of LCCs with high cell viability. We confirmed that the advanced 3D cell-printing system was capable of providing enhanced printability of hydrogels and fabricating an ear-shaped LCC with high cell viability. In vivo results for the ear-shaped LCC also showed that printed chondrocytes proliferated sufficiently and differentiated into cartilage tissue. Thus, we conclude that the advanced 3D cell-printing system is a versatile tool to create cell-printed constructs for the generation of large-volume tissues.

Scale-up laccase production from Trametes versicolor stimulated by vanillic acid.

PubMed

Wang, Ke-Feng; Hu, Jian-Hua; Guo, Chen; Liu, Chun-Zhao

2016-07-01

An efficient strategy for laccase production in Trametes versicolor cultures was developed using vanillic acid as the inducer. The optimized vanillic acid treatment strategy consisted of exposing 2-day-old mycelia cultures to 80 mg/L vanillic acid. After 4 days, laccase activity of 588.84 U/L was achieved in flasks which represented a 1.79-fold increase compared to the control. In 200-L airlift bioreactor, the maximal laccase activity reached up to 785.12 U/L using the optimized vanillic acid treatment strategy. The zymograms of culture supernatants revealed three bands with laccase activity, among which Lac1 and Lac2 were abundant laccase isoforms constitutively expressed, and Lac3 was an inducible isozyme by vanillic acid. The results of real-time quantitative PCR showed that the transcription level of lcc in T. versicolor cultures grown with vanillic acid for 7 days was about 5.64-fold greater than that without vanillic acid in flasks. In 200-L airlift bioreactor cultures of T. versicolor with addition of vanillic acid, the transcript level of lcc at day 7 was 2.62-fold higher than that in flasks with vanillic acid due to the good mass transfer and oxygen supply in the bioreactor system. This study provides a basis for understanding the induction mechanism of vanillic acid for laccase production and has good potential for industrial applications.

Adolescent and Young Adult Perceptions of Hookah and Little Cigars/Cigarillos: Implications for Risk Messages.

PubMed

Cornacchione, Jennifer; Wagoner, Kimberly G; Wiseman, Kimberly D; Kelley, Dannielle; Noar, Seth M; Smith, Margaret H; Sutfin, Erin L

2016-07-01

Use of hookah and little cigars/cigarillos (LCCs) is high among adolescents and young adults. Although these products have health effects similar to cigarettes, adolescents and young adults believe them to be safer. This study examined adolescent and young adult perceptions of hookah and LCCs to develop risk messages aimed at discouraging use among users and at-risk nonusers. Ten focus groups with 77 adolescents and young adults were conducted to explore their perceptions about the perceived risks and benefits of hookah and LCC use. Participants were users of other (non-cigarette) tobacco products (n = 47) and susceptible nonusers (n = 30). Transcripts were coded for emergent themes on participants' perceptions of hookah and LCCs. Participants did not perceive health effects associated with hookah and LCC use to be serious or likely to happen given their infrequency of use and perceptions that they are less harmful than cigarettes. Participants generally had positive associations with smoking hookah and LCCs for several reasons, including that they are used in social gatherings, come in various flavors, and can be used to perform smoke tricks. Because adolescents and young adults underestimate and discount the long-term risks associated with hookah and LCC use, effective messages may be those that focus on the acute/immediate health and cosmetic effects.

Robotics and Automation for Flight Deck Aircraft Servicing

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chesser, J.B.; Draper, J.V.; Pin, F.G.

1999-03-01

One of the missions of the Future Aircraft Carriers Program is to investigate methods that would improve aircraft turnaround servicing activities on carrier decks. The major objectives and criteria for evaluating alternative aircraft servicing methods are to reduce workload requirements, turnaround times (TAT), and life-cycle costs (LCC). Technologies in the field of Robotics and Automation (R and A) have the potential to significantly contribute to these objectives. The objective of this study was to investigate aircraft servicing functions on carrier decks which would offer the potentially most significant payoff if improved by various R and A technologies. Improvement in thismore » case means reducing workload, time and LCC. This objective was accomplished using a ''bottom-up'' formalized approach as described in the following.« less

[Letters] LightSquared Subsidiary LCC.

DOT National Transportation Integrated Search

2014-07-01

For consideration in the above-referenced pending proceeding before the Federal : Communications Commission (FCC), the National Telecommunications and Information : Administration (NTIA) forwards for inclusion in the record the enclosed letter from t...

Next-Generation Sequencing of a Cohort of Pulmonary Large Cell Carcinomas Reclassified by World Health Organization 2015 Criteria.

PubMed

Driver, Brandon R; Portier, Bryce P; Mody, Dina R; Deavers, Michael; Bernicker, Eric H; Kim, Min P; Teh, Bin S; Santacruz, Jose F; Kopas, Lisa; Munden, Reginald F; Cagle, Philip T

2016-04-01

The classification of pulmonary large cell carcinoma has undergone a major revision with the recent World Health Organization (WHO) 2015 Classification. Many large cell carcinomas are now reassigned to either adenocarcinoma with solid pattern or nonkeratinizing squamous cell carcinoma based on immunopositivity for adenocarcinoma markers or squamous cell carcinoma markers, respectively. Large cell carcinomas that are negative for adenocarcinoma and squamous cell carcinoma immunomarkers are now classified as large cell carcinoma with null immunohistochemical features (LCC-N). Although a few studies investigated the mutation profile of large cell carcinomas grouped by immunostain profile before the publication of the new WHO classification, investigation of tumors previously diagnosed as large cell carcinoma and reclassified according to the 2015 WHO classification has not, to our knowledge, been reported. To determine the mutation profiles of pulmonary large cell carcinomas reclassified by WHO 2015 criteria. Archival cases of non-small cell lung carcinoma with large cell carcinoma morphology (n = 17) were reclassified according to 2015 WHO criteria. To determine mutation profile, we employed Ion Torrent (Life Technologies, Carlsbad, California)-based next-generation sequencing (50 genes; more than 2800 mutations) in addition to real-time quantitative reverse transcription polymerase chain reaction for ALK translocation detection. Two of 17 cases (12%) were reclassified as LCC-N, and both had mutations-BRAF D594N in one case and KRAS G12C in the other case. Seven of 17 cases (41%) were reclassified in the adenocarcinoma with solid pattern group, which showed one KRAS G12C and one EGFR E709K + G719C double mutation in addition to mutations in TP53. Eight of 17 cases (47%) were reclassified in the nonkeratinizing squamous cell carcinoma group, which showed mutations in PIK3CA, CDKN2A, and TP53. No ALK translocations or amplifications were detected. The adenocarcinoma with solid pattern group showed mutations typical of adenocarcinoma, whereas the nonkeratinizing squamous cell carcinoma group showed mutations typical of squamous cell carcinoma. Both LCC-N cases had mutations associated with adenocarcinoma, supporting the hypothesis that LCC-N is related to adenocarcinoma.

Effects of Mental Fatigue on Physical Endurance Performance and Muscle Activation Are Attenuated by Monetary Incentives.

PubMed

Brown, Denver M Y; Bray, Steven R

2017-12-01

Physical performance is impaired following cognitive control exertion. Incentives can ameliorate adverse carryover effects of cognitive control exertion but have not been investigated for physical endurance. This study examined the effect of monetary incentives on physical performance and muscle activation following exposure to a mentally fatiguing, cognitive control task. Participants (N = 82) performed two isometric endurance handgrip trials separated by a 12-min cognitive control manipulation using a 2 (high cognitive control [HCC]/low cognitive control [LCC]) × 2 (incentive/no incentive) design. Mental fatigue was significantly higher in the HCC conditions. Performance decreased in the HCC/no incentive condition but was unaffected in the HCC/incentive condition, which did not differ from the low cognitive control conditions. Electromyography data revealed increased muscle activation in the HCC/no incentive condition, which was also attenuated in the HCC/incentive condition. Findings show that incentives counteract the negative effects of HCC on physical endurance and alter central drive to motor units.

Simplified Life-Cycle Cost Estimation

NASA Technical Reports Server (NTRS)

Remer, D. S.; Lorden, G.; Eisenberger, I.

1983-01-01

Simple method for life-cycle cost (LCC) estimation avoids pitfalls inherent in formulations requiring separate estimates of inflation and interest rates. Method depends for validity observation that interest and inflation rates closely track each other.

Local Laplacian Coding From Theoretical Analysis of Local Coding Schemes for Locally Linear Classification.

PubMed

Pang, Junbiao; Qin, Lei; Zhang, Chunjie; Zhang, Weigang; Huang, Qingming; Yin, Baocai

2015-12-01

Local coordinate coding (LCC) is a framework to approximate a Lipschitz smooth function by combining linear functions into a nonlinear one. For locally linear classification, LCC requires a coding scheme that heavily determines the nonlinear approximation ability, posing two main challenges: 1) the locality making faraway anchors have smaller influences on current data and 2) the flexibility balancing well between the reconstruction of current data and the locality. In this paper, we address the problem from the theoretical analysis of the simplest local coding schemes, i.e., local Gaussian coding and local student coding, and propose local Laplacian coding (LPC) to achieve the locality and the flexibility. We apply LPC into locally linear classifiers to solve diverse classification tasks. The comparable or exceeded performances of state-of-the-art methods demonstrate the effectiveness of the proposed method.

Life cycle cost assessment of future low heat rejection engines

NASA Technical Reports Server (NTRS)

Petersen, D. R.

1986-01-01

The Adiabatic Diesel Engine Component Development (ADECD) represents a project which has the objective to accelerate the development of highway truck engines with advanced technology aimed at reduced fuel consumption. The project comprises three steps, including the synthesis of a number of engine candidate designs, the coupling of each with a number of systems for utilizing exhaust gas energy, and the evaluation of each combination in terms of desirability. Particular attention is given to the employed evaluation method and the development of this method. The objective of Life Cycle Cost (LCC) evaluation in the ADECD program was to select the best from among 42 different low heat rejection engine (LHRE)/exhaust energy recovery system configurations. The LCC model is discussed along with a maintenance cost model, the evaluation strategy, the selection of parameter ranges, and a full factorial analysis.

Tobacco Industry Use of Flavours to Recruit New Users of Little Cigars and Cigarillos

PubMed Central

Kostygina, Ganna; Glantz, Stanton A.; Ling, Pamela M.

2014-01-01

Objective While flavoured cigarettes were prohibited in the US in 2009, flavoured little cigars and cigarillos (LCCs) remain on the market. We describe the evolving strategies used by tobacco companies to encourage uptake of flavoured little cigars and cigarillos and industry research findings on consumer perceptions of flavoured LCC products. Methods Analysis of internal tobacco industry documents was triangulated with data from tobacco advertisement archives, national newspapers, trade press, and the Internet. Results Flavoured little cigar and cigarillo products were associated with young and inexperienced tobacco users, women and African Americans. Internal industry studies confirmed that menthol and candy-like flavours (e.g., vanilla, cherry) increased LCC appeal to starters by masking the heavy cigar taste, reducing throat irritation, and making LCC smoke easier to inhale. To appeal to new users, manufacturers also reduced the size of cigars to make them more cigarette-like, introduced filters and flavoured filter tips, emphasized mildness and ease of draw in advertising, and featured actors using little cigars in television commercials. RJ Reynolds tried to capitalize on the popularity of menthol cigarettes among African Americans and marketed a menthol little cigar to African Americans. Conclusions Tobacco companies engaged in a calculated effort to blur the line between little cigars and cigarettes to increase appeal to cigarette smokers, and the use of flavours facilitated these efforts. Bans on flavoured cigarettes should be expanded to include flavoured LCCs, and tobacco use prevention initiatives should include LCCs. PMID:25354674

Life Cycle Cost Growth Study for the Discovery and New Frontiers Program Office

NASA Technical Reports Server (NTRS)

Barley, Bryan; Gilbert, Paul; Newhouse, Marilyn

2010-01-01

The D&NF Program Office LCC Management Study provides a detailed look at the drivers underlying cost overruns and schedule delays for five D&NF missions. While none of the findings are new, the study underlines the importance of continued emphasis on sound project management techniques: a clean project management structure with a clear definition of roles and responsibilities across the various partners in a project, an understanding of institutional standards and procedures and any differences among the partners, and the critical need for a comprehensive IMS that can be used easily and routinely to identify potential threats to the critical path. The study also highlights the continuing need for realistic estimates of the total LCC. Sufficient time and resources must be allocated early in a project to ensure that the appropriate trade studies and analyses are performed across all aspects of a mission: spacecraft, ground system, operations concept, and fault management, to ensure that proposed and confirmed costs truly reflect the resource requirements over the entire mission life cycle. These studies need to include a realistic review of the assumptions underlying the use of new technologies, the integration of heritage and new hardware and software into the total mission environment, and any development and test savings based on heritage technology and lessons learned. Finally, the LCC Management Study stresses the need to listen to, carefully consider, and take positive action regarding the issues raised during reviews by the expert review teams.

A study on stimulation of DC high voltage power of LCC series parallel resonant in projectile velocity measurement system

NASA Astrophysics Data System (ADS)

Lu, Dong-dong; Gu, Jin-liang; Luo, Hong-e.; Xia, Yan

2017-10-01

According to specific requirements of the X-ray machine system for measuring velocity of outfield projectile, a DC high voltage power supply system is designed for the high voltage or the smaller current. The system comprises: a series resonant circuit is selected as a full-bridge inverter circuit; a high-frequency zero-current soft switching of a high-voltage power supply is realized by PWM output by STM32; a nanocrystalline alloy transformer is chosen as a high-frequency booster transformer; and the related parameters of an LCC series-parallel resonant are determined according to the preset parameters of the transformer. The concrete method includes: a LCC series parallel resonant circuit and a voltage doubling circuit are stimulated by using MULTISM and MATLAB; selecting an optimal solution and an optimal parameter of all parts after stimulation analysis; and finally verifying the correctness of the parameter by stimulation of the whole system. Through stimulation analysis, the output voltage of the series-parallel resonant circuit gets to 10KV in 28s: then passing through the voltage doubling circuit, the output voltage gets to 120KV in one hour. According to the system, the wave range of the output voltage is so small as to provide the stable X-ray supply for the X-ray machine for measuring velocity of outfield projectile. It is fast in charging and high in efficiency.

Quantifying capture stress in free ranging European roe deer (Capreolus capreolus).

PubMed

Huber, Nikolaus; Vetter, Sebastian G; Evans, Alina L; Kjellander, Petter; Küker, Susanne; Bergvall, Ulrika A; Arnemo, Jon M

2017-05-10

To understand and reduce the concomitant effects of trapping and handling procedures in wildlife species, it is essential to measure their physiological impact. Here, we examined individual variation in stress levels in non-anesthetized European roe deer (Capreolus capreolus), which were captured in box traps and physically restrained for tagging, biometrics and bio-sampling. In winter 2013, we collected venous blood samples from 28 individuals during 28 capture events and evaluated standard measurements for stress (heart rate, body temperature, neutrophil to lymphocyte ratio, lactate and total cortisol). Additionally, we assessed stress using the immunological tool, Leukocyte Coping Capacity (LCC), a real-time proxy for stress measuring oxygen radical production by leukocytes. Finally, the behavioral response to handling was recorded using a scoring system. LCC and therefore stress levels were negatively influenced by the time animals spent in the box trap with human presence at the capture site prior to handling. In contrast, none of the classical stress measures, including total cortisol, nor the behavioral assessment, were correlated with the stressor tested (time of human presence prior to handling) and thus did not provide a clear depiction regarding the extent of the animals short-term stress response. Overall our study verifies the LCC as a strong method to quantify short-term stress reactions in wildlife. Moreover, our results clearly show that human presence at the trapping site prior to handling should be kept to an absolute minimum in order to reduce stress levels.

Two-scale evaluation of remediation technologies for a contaminated site by applying economic input-output life cycle assessment: risk-cost, risk-energy consumption and risk-CO2 emission.

PubMed

Inoue, Yasushi; Katayama, Arata

2011-09-15

A two-scale evaluation concept of remediation technologies for a contaminated site was expanded by introducing life cycle costing (LCC) and economic input-output life cycle assessment (EIO-LCA). The expanded evaluation index, the rescue number for soil (RN(SOIL)) with LCC and EIO-LCA, comprises two scales, such as risk-cost, risk-energy consumption or risk-CO(2) emission of a remediation. The effectiveness of RN(SOIL) with LCC and EIO-LCA was examined in a typical contamination and remediation scenario in which dieldrin contaminated an agricultural field. Remediation was simulated using four technologies: disposal, high temperature thermal desorption, biopile and landfarming. Energy consumption and CO(2) emission were determined from a life cycle inventory analysis using monetary-based intensity based on an input-output table. The values of RN(SOIL) based on risk-cost, risk-energy consumption and risk-CO(2) emission were calculated, and then rankings of the candidates were compiled according to RN(SOIL) values. A comparison between three rankings showed the different ranking orders. The existence of differences in ranking order indicates that the scales would not have reciprocal compatibility for two-scale evaluation and that each scale should be used independently. The RN(SOIL) with LCA will be helpful in selecting a technology, provided an appropriate scale is determined. Copyright © 2011 Elsevier B.V. All rights reserved.

Overexpression and characterization of laccase from Trametes versicolor in Pichia pastoris.

PubMed

Li, Q; Pei, J; Zhao, L; Xie, J; Cao, F; Wang, G

2014-01-01

A laccase-encoding gene of Trametes versicolor, lccA, was cloned and expressed in Pichia pastoris X33. The lccA gene consists ofa 1560 bp open reading frame encoding 519 amino acids, which was classified into family copper blue oxidase. To improve the expression level of recombinant laccase in P. pastoris, conditions of the fermentation were optimized by the single factor experiments. The optimal fermentation conditions for the laccase production in shake flask cultivation using BMGY medium were obtained: the optimal initial pH 7.0, the presence of 0.5 mM Cu2+, 0.6% methanol added into the culture every 24 h. The laccase activity was up to 11.972 U/L under optimal conditions after 16 days of induction in a medium with 4% peptone. After 100 h of large scale production in 5 L fermenter the enzyme activity reached 18.123 U/L. The recombinant laccase was purified by ultrafiltration and (NH4)2SO4 precipitation showing a single band on SDS-PAGE, which had a molecular mass of 58 kDa. The optimum pH and temperature for the laccase were pH 2.0 and 50 degrees C with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) as a substrate. The recombinant laccase was stable over a pH range of 2.0-7.0. The K(m) and the V(max) value of LccA were 0.43 mM and 82.3 U/mg for ABTS, respectively.

Analysis and Design of High-Order Parallel Resonant Converters

NASA Astrophysics Data System (ADS)

Batarseh, Issa Eid

1990-01-01

In this thesis, a special state variable transformation technique has been derived for the analysis of high order dc-to-dc resonant converters. Converters comprised of high order resonant tanks have the advantage of utilizing the parasitic elements by making them part of the resonant tank. A new set of state variables is defined in order to make use of two-dimensional state-plane diagrams in the analysis of high order converters. Such a method has been successfully used for the analysis of the conventional Parallel Resonant Converters (PRC). Consequently, two -dimensional state-plane diagrams are used to analyze the steady state response for third and fourth order PRC's when these converters are operated in the continuous conduction mode. Based on this analysis, a set of control characteristic curves for the LCC-, LLC- and LLCC-type PRC are presented from which various converter design parameters are obtained. Various design curves for component value selections and device ratings are given. This analysis of high order resonant converters shows that the addition of the reactive components to the resonant tank results in converters with better performance characteristics when compared with the conventional second order PRC. Complete design procedure along with design examples for 2nd, 3rd and 4th order converters are presented. Practical power supply units, normally used for computer applications, were built and tested by using the LCC-, LLC- and LLCC-type commutation schemes. In addition, computer simulation results are presented for these converters in order to verify the theoretical results.

Interactive effects of climate change with nutrients, mercury, and freshwater acidification on key taxa in the North Atlantic Landscape Conservation Cooperative region.

PubMed

Pinkney, Alfred E; Driscoll, Charles T; Evers, David C; Hooper, Michael J; Horan, Jeffrey; Jones, Jess W; Lazarus, Rebecca S; Marshall, Harold G; Milliken, Andrew; Rattner, Barnett A; Schmerfeld, John; Sparling, Donald W

2015-07-01

The North Atlantic Landscape Conservation Cooperative LCC (NA LCC) is a public-private partnership that provides information to support conservation decisions that may be affected by global climate change (GCC) and other threats. The NA LCC region extends from southeast Virginia to the Canadian Maritime Provinces. Within this region, the US National Climate Assessment documented increases in air temperature, total precipitation, frequency of heavy precipitation events, and rising sea level, and predicted more drastic changes. Here, we synthesize literature on the effects of GCC interacting with selected contaminant, nutrient, and environmental processes to adversely affect natural resources within this region. Using a case study approach, we focused on 3 stressors with sufficient NA LCC region-specific information for an informed discussion. We describe GCC interactions with a contaminant (Hg) and 2 complex environmental phenomena-freshwater acidification and eutrophication. We also prepared taxa case studies on GCC- and GCC-contaminant/nutrient/process effects on amphibians and freshwater mussels. Several avian species of high conservation concern have blood Hg concentrations that have been associated with reduced nesting success. Freshwater acidification has adversely affected terrestrial and aquatic ecosystems in the Adirondacks and other areas of the region that are slowly recovering due to decreased emissions of N and sulfur oxides. Eutrophication in many estuaries within the region is projected to increase from greater storm runoff and less denitrification in riparian wetlands. Estuarine hypoxia may be exacerbated by increased stratification. Elevated water temperature favors algal species that produce harmful algal blooms (HABs). In several of the region's estuaries, HABs have been associated with bird die-offs. In the NA LCC region, amphibian populations appear to be declining. Some species may be adversely affected by GCC through higher temperatures and more frequent droughts. GCC may affect freshwater mussel populations via altered stream temperatures and increased sediment loading during heavy storms. Freshwater mussels are sensitive to un-ionized ammonia that more toxic at higher temperatures. We recommend studying the interactive effects of GCC on generation and bioavailability of methylmercury and how GCC-driven shifts in bird species distributions will affect avian exposure to methylmercury. Research is needed on how decreases in acid deposition concurrent with GCC will alter the structure and function of sensitive watersheds and surface waters. Studies are needed to determine how GCC will affect HABs and avian disease, and how more severe and extensive hypoxia will affect fish and shellfish populations. Regarding amphibians, we suggest research on 1) thermal tolerance and moisture requirements of species of concern, 2) effects of multiple stressors (temperature, desiccation, contaminants, nutrients), and 3) approaches to mitigate impacts of increased temperature and seasonal drought. We recommend studies to assess which mussel species and populations are vulnerable and which are resilient to rising stream temperatures, hydrological shifts, and ionic pollutants, all of which are influenced by GCC. © 2015 SETAC.

Interactive effects of climate change with nutrients, mercury, and freshwater acidification on key taxa in the North Atlantic Landscape Conservation Cooperative region

USGS Publications Warehouse

Pinkney, Alfred E.; Driscoll, Charles T.; Evers, David C.; Hooper, Michael J.; Horan, Jeffrey; Jones, Jess W.; Lazarus, Rebecca S.; Marshall, Harold G.; Milliken, Andrew; Rattner, Barnett A.; Schmerfeld, John J.; Sparling, Donald W.

2015-01-01

The North Atlantic Landscape Conservation Cooperative LCC (NA LCC) is a public–private partnership that provides information to support conservation decisions that may be affected by global climate change (GCC) and other threats. The NA LCC region extends from southeast Virginia to the Canadian Maritime Provinces. Within this region, the US National Climate Assessment documented increases in air temperature, total precipitation, frequency of heavy precipitation events, and rising sea level, and predicted more drastic changes. Here, we synthesize literature on the effects of GCC interacting with selected contaminant, nutrient, and environmental processes to adversely affect natural resources within this region. Using a case study approach, we focused on 3 stressors with sufficient NA LCC region-specific information for an informed discussion. We describe GCC interactions with a contaminant (Hg) and 2 complex environmental phenomena—freshwater acidification and eutrophication. We also prepared taxa case studies on GCC- and GCC-contaminant/nutrient/process effects on amphibians and freshwater mussels. Several avian species of high conservation concern have blood Hg concentrations that have been associated with reduced nesting success. Freshwater acidification has adversely affected terrestrial and aquatic ecosystems in the Adirondacks and other areas of the region that are slowly recovering due to decreased emissions of N and sulfur oxides. Eutrophication in many estuaries within the region is projected to increase from greater storm runoff and less denitrification in riparian wetlands. Estuarine hypoxia may be exacerbated by increased stratification. Elevated water temperature favors algal species that produce harmful algal blooms (HABs). In several of the region's estuaries, HABs have been associated with bird die-offs. In the NA LCC region, amphibian populations appear to be declining. Some species may be adversely affected by GCC through higher temperatures and more frequent droughts. GCC may affect freshwater mussel populations via altered stream temperatures and increased sediment loading during heavy storms. Freshwater mussels are sensitive to un-ionized ammonia that more toxic at higher temperatures. We recommend studying the interactive effects of GCC on generation and bioavailability of methylmercury and how GCC-driven shifts in bird species distributions will affect avian exposure to methylmercury. Research is needed on how decreases in acid deposition concurrent with GCC will alter the structure and function of sensitive watersheds and surface waters. Studies are needed to determine how GCC will affect HABs and avian disease, and how more severe and extensive hypoxia will affect fish and shellfish populations. Regarding amphibians, we suggest research on 1) thermal tolerance and moisture requirements of species of concern, 2) effects of multiple stressors (temperature, desiccation, contaminants, nutrients), and 3) approaches to mitigate impacts of increased temperature and seasonal drought. We recommend studies to assess which mussel species and populations are vulnerable and which are resilient to rising stream temperatures, hydrological shifts, and ionic pollutants, all of which are influenced by GCC.

Research on the optimization of quota design in real estate

NASA Astrophysics Data System (ADS)

Sun, Chunling; Ma, Susu; Zhong, Weichao

2017-11-01

Quota design is one of the effective methods of cost control in real estate development project and widely used in the current real estate development project to control the engineering construction cost, but quota design have many deficiencies in design process. For this purpose, this paper put forward a method to achieve investment control of real estate development project, which combine quota design and value engineering(VE) at the stage of design. Specifically, it’s an optimizing for the structure of quota design. At first, determine the design limits by investment estimate value, then using VE to carry on initial allocation of design limits and gain the functional target cost, finally, consider the whole life cycle cost (LCC) and operational problem in practical application to finish complex correction for the functional target cost. The improved process can control the project cost more effectively. It not only can control investment in a certain range, but also make the project realize maximum value within investment.

Liposome-based drug co-delivery systems in cancer cells.

PubMed

Zununi Vahed, Sepideh; Salehi, Roya; Davaran, Soodabeh; Sharifi, Simin

2017-02-01

Combination therapy and nanotechnology offer a promising therapeutic method in cancer treatment. By improving drug's pharmacokinetics, nanoparticulate systems increase the drug's therapeutic effects while decreasing its adverse side effects related to high dosage. Liposomes are extensively used as drug delivery systems and several liposomal nanomedicines have been approved for clinical applications. In this regard, liposome-based combination chemotherapy (LCC) opens a novel avenue in drug delivery research and has increasingly become a significant approach in clinical cancer treatment. This review paper focuses on LCC strategies including co-delivery of: two chemotherapeutic drugs, chemotherapeutic agent with anti-cancer metals, and chemotherapeutic agent with gene agents and ligand-targeted liposome for co-delivery of chemotherapeutic agents. Definitely, the multidisciplinary method may help improve the efficacy of cancer therapy. An extensive literature review was performed mainly using PubMed. Copyright © 2016 Elsevier B.V. All rights reserved.

Melanoidin-containing wastewaters induce selective laccase gene expression in the white-rot fungus Trametes sp. I-62.

PubMed

González, Tania; Terrón, María Carmen; Yagüe, Susana; Junca, Howard; Carbajo, José María; Zapico, Ernesto Javier; Silva, Ricardo; Arana-Cuenca, Ainhoa; Téllez, Alejandro; González, Aldo Enrique

2008-03-01

Wastewaters generated from the production of ethanol from sugar cane molasses may have detrimental effects on the environment due to their high chemical oxygen demand and dark brown color. The color is mainly associated with the presence of melanoidins, which are highly recalcitrant to biodegradation. We report here the induction of laccases by molasses wastewaters and molasses melanoidins in the basidiomycetous fungus Trametes sp. I-62. The time course of effluent decolorization and laccase activity in the culture supernatant of the fungus were correlated. The expression of laccase genes lcc1 and lcc2 increased as a result of the addition of complete molasses wastewater and its high molecular weight fraction to fungal cultures. This is the first time differential laccase gene expression has been reported to occur upon exposure of fungal cultures to molasses wastewaters and their melanoidins.

Optimization of the expression of a laccase gene from Trametes versicolor in Pichia methanolica.

PubMed

Guo, Mei; Lu, Fuping; Du, Lianxiang; Pu, Jun; Bai, Dongqing

2006-08-01

A cDNA encoding for laccase (Lcc1) was isolated from the ligninolytic fungus Trametes versicolor by reverse transcriptase polymerase chain reaction. The Lcc1 gene was subcloned into the Pichia methanolica expression vector pMETalphaA and transformed into the P. methanolica strains PMAD11 and PMAD16. The extracellular laccase activity of the PMAD11 recombinants was found to be 1.3-fold higher than that of the PMAD16 recombinants. The identity of the recombinant protein was further confirmed by immunodetection using the Western blot analysis. As expected, the molecular mass of the mature laccase was 64.0 kDa, similar to that of the native form. The effects of copper concentration, cultivation temperature, pH and methanol concentration in the BMMY on laccase expression were investigated. The laccase activity in the PMAD11 recombinant was up to 12.6 U ml(-1) by optimization.

A Novel Remote Sensing Approach for Prediction of Maize Yield Under Different Conditions of Nitrogen Fertilization

PubMed Central

Vergara-Díaz, Omar; Zaman-Allah, Mainassara A.; Masuka, Benhildah; Hornero, Alberto; Zarco-Tejada, Pablo; Prasanna, Boddupalli M.; Cairns, Jill E.; Araus, José L.

2016-01-01

Maize crop production is constrained worldwide by nitrogen (N) availability and particularly in poor tropical and subtropical soils. The development of affordable high-throughput crop monitoring and phenotyping techniques is key to improving maize cultivation under low-N fertilization. In this study several vegetation indices (VIs) derived from Red-Green-Blue (RGB) digital images at the leaf and canopy levels are proposed as low-cost tools for plant breeding and fertilization management. They were compared with the performance of the normalized difference vegetation index (NDVI) measured at ground level and from an aerial platform, as well as with leaf chlorophyll content (LCC) and other leaf composition and structural parameters at flowering stage. A set of 10 hybrids grown under five different nitrogen regimes and adequate water conditions were tested at the CIMMYT station of Harare (Zimbabwe). Grain yield and leaf N concentration across N fertilization levels were strongly predicted by most of these RGB indices (with R2~ 0.7), outperforming the prediction power of the NDVI and LCC. RGB indices also outperformed the NDVI when assessing genotypic differences in grain yield and leaf N concentration within a given level of N fertilization. The best predictor of leaf N concentration across the five N regimes was LCC but its performance within N treatments was inefficient. The leaf traits evaluated also seemed inefficient as phenotyping parameters. It is concluded that the adoption of RGB-based phenotyping techniques may significantly contribute to the progress of plant breeding and the appropriate management of fertilization. PMID:27242867

Fit-for-purpose wastewater treatment: Testing to implementation of decision support tool (II).

PubMed

Chhipi-Shrestha, Gyan; Hewage, Kasun; Sadiq, Rehan

2017-12-31

This paper is the second in a series of two papers. In Paper I, a decision support tool (DST), FitWater, was developed for evaluating the potential of wastewater treatment (WWT) trains for various water reuse applications. In the present paper, the proposed DST has been tested and implemented. FitWater has been tested with several existing WWT plants in Canada and the USA, demonstrating FitWater's effectiveness in estimating life cycle cost (LCC), health risk, and energy use. FitWater has also been implemented in a newly planned neighbourhood in the Okanagan Valley (BC, Canada) by developing 12 alternative WWT trains for water reuse in lawn and public parks irrigation. The results show that FitWater can effectively rank WWT train alternatives based on LCC, health risk, amount of reclaimed water, energy use, and carbon emissions. Moreover, functions have been developed for the variation of unit annualized LCC and energy intensity per unit log removal of microorganisms in different treatment technologies with varying plant capacities. The functions have power relations, showing the economies of scale. FitWater can be applied to identify a cost-effective, risk-acceptable, and energy efficient wastewater treatment train with a plant capacity of 500m 3 /day or more. Furthermore, FitWater can be used to assess potential economic impacts of developing microbiologically stringent effluent standards. The capability of FitWater can be enhanced by including physio-chemical quality of wastewater, additional treatment technologies, and carbon emissions from wastewater decomposition processes. Copyright © 2017 Elsevier B.V. All rights reserved.

Does duration of caffeine therapy in preterm infants born ≤1250 g at birth influence neurodevelopmental (ND) outcomes at 3 years of age?

PubMed

Lodha, A; Rabi, Y; Soraisham, A; Dobry, J; Lodha, Arijit; Amin, H; Awad, E Al; Tang, S; Sahai, A; Bhandari, V

2018-05-08

To evaluate the effect of duration of caffeine use on long-term neurodevelopmental (ND) outcomes at 3 years corrected age (CA) in preterm infants with birthweights (BW) ≤ 1250 g. All surviving infants with BW ≤ 1250 g admitted to the Foothills Medical Center neonatal intensive care unit (NICU) from January 2002 to December 2009 who received the first dose of caffeine in the first week of life and were followed up at three years CA were included in the study. Demographics and follow-up outcomes were compared based on early cessation of caffeine ≤ 14 days (ECC), intermediate cessation of caffeine 15-30 days (ICC), and late cessation of caffeine >30 days (LCC). The primary outcome of ND impairment was present if a child had any one of the following: cerebral palsy, cognitive delay, visual impairment, or hearing impairment or deafness. Univariate and logistic regression analyses were performed. Of the 508 eligible infants, 448 (88%) were seen at 3 years CA at follow-up. ECC (n = 139), ICC (n = 122) and LCC (n = 187) groups had a median (range) BW of 979 (560-1250), 1010 (530-1250), and 980 (520-1250) g (p = 0.524) and median (range) gestational age (GA) of 27 (23-33), 28 (24-33), and 27 (24-32) weeks, respectively (p = 0.034). In logistic regression models adjusting for GA, maternal smoking, and each neonatal risk factor separately (IVH, NEC, sepsis, blood transfusions, BPD, postnatal dexamethasone, SNAP-II, and ventilator days), none of the models showed a statistically significant association between caffeine duration and ND impairment. The duration of caffeine use in premature infants in the NICU does not impact on long-term ND outcomes at 3 years CA.

Development challenges of secondary and small airports in California.

DOT National Transportation Integrated Search

2012-06-01

This study investigates the development of secondary and smaller airports in California. Low-Cost Carrier (LCC) business is growing at these airports because they offer reduced operating costs, and they have adequate capacity to help LCCs avoid battl...

Development challenges of secondary and small airports in California [research brief].

DOT National Transportation Integrated Search

2012-06-01

This report investigates the challenges facing secondary and small airports in California. Low-cost carriers (LCC) have slowly risen to become the dominant air service providers in the state. Their dominance and their unique business model have signi...

Patterns of combustible tobacco use in U.S. young adults and potential response to graphic cigarette health warning labels.

PubMed

Villanti, Andrea C; Pearson, Jennifer L; Cantrell, Jennifer; Vallone, Donna M; Rath, Jessica M

2015-03-01

In the evolving landscape of tobacco use, it remains unclear how tobacco control efforts should be designed and promoted for maximum impact. The current study links the identification of latent classes of young adult combustible tobacco users with anticipated responses to graphic health warning labels (HWLs). Data were collected in January 2012 using an online address-based panel as part of the Legacy Young Adult Cohort Study, and analyses were conducted in 2013. Latent class analyses identified five groups of tobacco users in a national sample of 4,236 young adults aged 18-34years: (1) little cigar/cigarillo/bidi (LCC) and hookah users (4%); (2) nonusers, open to smoking (3%); (3) daily smokers who self-identify as "smokers" (11%); (4) nondaily, light smokers who self-identify as "social or occasional smokers" (9%); and (5) nonusers closed to smoking (73%). Of the nonusers closed to smoking, 23% may be better characterized as at risk for tobacco initiation. Results indicate differences in the potential effectiveness of HWLs across classes. Compared to the daily "smokers," LCC and hookah users (RRR=2.35) and nonusers closed to smoking (RRR=2.33) were more than twice as likely to report that new graphic HWLs would make them think about not smoking. This study supports the potential of graphic HWLs to prevent young nonusers from using tobacco products. It suggests that the extension of prominent HWLs to other tobacco products, including LCCs and hookah tobacco, may also serve a prevention function. Copyright © 2014 Elsevier Ltd. All rights reserved.

Network Systems Administration Needs Assessment.

ERIC Educational Resources Information Center

Lexington Community Coll., KY. Office of Institutional Research.

In spring 1996, Lexington Community College (LCC) in Kentucky, conducted a survey to gather information on employment trends and educational needs in the field of network systems administration (NSA). NSA duties involve the installation and administration of network operating systems, applications software, and networking infrastructure;…

@Caribbean_LCC | CARIBBEAN LANDSCAPE CONSERVATION COOPERATIVE (A2)

Science.gov Websites

Monitoring Data Ecosystem Governance Community Get involved Advisory Groups Scientific Community Practitioner ! Caribbean Agriculture, Forestry and Climate Governance Database Slide background LANDSCAPE Conservation Is Caribbean. Ecosystem Governance Discover our compendium of NGOs and coalition groups doing conservation

Customized Training Marketing Plan.

ERIC Educational Resources Information Center

Lay, Ted

This report outlines Oregon's Lane Community College's (LCC's) plan for marketing its customized training program for business, community organizations, public agencies, and their employees. Following a mission statement for the customized training program, a brief analysis is provided of the economic environment; of competition from educational…

Does Evapotranspiration Increase When Forests are converted to Grasslands?

NASA Astrophysics Data System (ADS)

Varcoe, Robert; Sterling, Shannon

2017-04-01

The conversion of forests to grasslands (FGC) is a widespread land cover change (LCC) and is also among the most commonly studied changes with respect to its impact on ET; such research employs a variety of experimental approaches, including, paired catchment (PC), Budyko and land surface models (LSM), and measurement methods, including the catchment water balance (CWB), eddy covariance (EC) and remote sensing (RS). Until recently, there has been consensus in the scientific literature that rates of ET decrease when a forest is converted to grassland; however, this consensus has recently come into question. Williams (2012) applied the Budyko framework to a global network of eddy covariance measurements with the results that grasslands have a 9% greater evaporative index than forests. In addition, HadGEM2, a recent Hadley Centre LSM, produced increased ET in the northern Amazon Basin after simulating global scale tropical deforestation (Brovkin et al., 2015). Here we present an analysis of available estimates of how ET rates change with FGC to increase our understanding of the forest - grassland-ET paradigm. We used two datasets to investigate the impacts land cover change on ET. I compiled a dataset of change in ET with land cover change (ΔETLCC) using published experiments that compare forest and grassland ET under conditions controlled for meteorological and landscape influences. Using the ΔETLCC dataset, we show that, in all cases, forest ET is higher than grassland under controlled conditions. Results suggest that the eddy covariance method measures smaller changes in ET when forests are converted to grasslands, though more data are needed for this result to be statistically significant. Finally, GETA2.0, a new global dataset of annual ET, projects that forest ET is greater than grassland, except at high latitudes and areas where orography influences precipitation (P). The data included in this study represent the data available on forest and grassland ET comparison and reveal an important gap in the scientific literature: the lack of data available regarding forest to grassland LCC.

Functional reconstitution of the Mycobacterium tuberculosis long-chain acyl-CoA carboxylase from multiple acyl-CoA subunits.

PubMed

Bazet Lyonnet, Bernardo; Diacovich, Lautaro; Gago, Gabriela; Spina, Lucie; Bardou, Fabienne; Lemassu, Anne; Quémard, Annaïk; Gramajo, Hugo

2017-04-01

Mycobacterium tuberculosis produces a large number of structurally diverse lipids that have been implicated in the pathogenicity, persistence and antibiotic resistance of this organism. Most building blocks involved in the biosynthesis of all these lipids are generated by acyl-CoA carboxylases whose subunit composition and physiological roles have not yet been clearly established. Inconclusive data in the literature refer to the exact protein composition and substrate specificity of the enzyme complex that produces the long-chain α-carboxy-acyl-CoAs, which are substrates involved in the last step of condensation mediated by the polyketide synthase 13 to synthesize mature mycolic acids. Here we have successfully reconstituted the long-chain acyl-CoA carboxylase (LCC) complex from its purified components, the α subunit (AccA3), the ε subunit (AccE5) and the two β subunits (AccD4 and AccD5), and demonstrated that the four subunits are essential for its activity. Furthermore, we also showed by substrate competition experiments and the use of a specific inhibitor that the AccD5 subunit's role in the carboxylation of the long acyl-CoAs, as part of the LCC complex, was structural rather than catalytic. Moreover, AccD5 was also able to carboxylate its natural substrates, acetyl-CoA and propionyl-CoA, in the context of the LCC enzyme complex. Thus, the supercomplex formed by these four subunits has the potential to generate the main substrates, malonyl-CoA, methylmalonyl-CoA and α-carboxy-C 24-26 -CoA, used as condensing units for the biosynthesis of all the lipids present in this pathogen. © 2017 Federation of European Biochemical Societies.

Construction and characterization of an in-vivo linear covalently closed DNA vector production system.

PubMed

Nafissi, Nafiseh; Slavcev, Roderick

2012-12-06

While safer than their viral counterparts, conventional non-viral gene delivery DNA vectors offer a limited safety profile. They often result in the delivery of unwanted prokaryotic sequences, antibiotic resistance genes, and the bacterial origins of replication to the target, which may lead to the stimulation of unwanted immunological responses due to their chimeric DNA composition. Such vectors may also impart the potential for chromosomal integration, thus potentiating oncogenesis. We sought to engineer an in vivo system for the quick and simple production of safer DNA vector alternatives that were devoid of non-transgene bacterial sequences and would lethally disrupt the host chromosome in the event of an unwanted vector integration event. We constructed a parent eukaryotic expression vector possessing a specialized manufactured multi-target site called "Super Sequence", and engineered E. coli cells (R-cell) that conditionally produce phage-derived recombinase Tel (PY54), TelN (N15), or Cre (P1). Passage of the parent plasmid vector through R-cells under optimized conditions, resulted in rapid, efficient, and one step in vivo generation of mini lcc--linear covalently closed (Tel/TelN-cell), or mini ccc--circular covalently closed (Cre-cell), DNA constructs, separated from the backbone plasmid DNA. Site-specific integration of lcc plasmids into the host chromosome resulted in chromosomal disruption and 10(5) fold lower viability than that seen with the ccc counterpart. We offer a high efficiency mini DNA vector production system that confers simple, rapid and scalable in vivo production of mini lcc DNA vectors that possess all the benefits of "minicircle" DNA vectors and virtually eliminate the potential for undesirable vector integration events.

A tool to evaluate local biophysical effects on temperature due to land cover change transitions

NASA Astrophysics Data System (ADS)

Perugini, Lucia; Caporaso, Luca; Duveiller, Gregory; Cescatti, Alessandro; Abad-Viñas, Raul; Grassi, Giacomo; Quesada, Benjamin

2017-04-01

Land Cover Changes (LCC) affect local, regional and global climate through biophysical variations of the surface energy budget mediated by albedo, evapotranspiration, and roughness. Assessment of the full climate impacts of anthropogenic LCC are incomplete without considering biophysical effects, but the high level of uncertainties in quantifying their impacts to date have made it impractical to offer clear advice on which policy makers could act. To overcome this barrier, we provide a tool to evaluate the biophysical impact of a matrix of land cover transitions, following a tiered methodological approach similar to the one provided by the IPCC to estimate the biogeochemical effects, i.e. through three levels of methodological complexity, from Tier 1 (i.e. default method and factors) to Tier 3 (i.e. specific methods and factors). In particular, the tool provides guidance for quantitative assessment of changes in temperature following a land cover transition. The tool focuses on temperature for two main reasons (i) it is the main variable of interest for policy makers at local and regional level, and (ii) temperature is able to summarize the impact of radiative and non-radiative processes following LULCC. The potential changes in annual air temperature that can be expected from various land cover transitions are derived from a dedicated dataset constructed by the JRC in the framework of the LUC4C FP7 project. The inputs for the dataset are air temperature values derived from satellite Earth Observation data (MODIS) and land cover characterization from the ESA Climate Change Initiative product reclassified into their IPCC land use category equivalent. This data, originally at 0.05 degree of spatial resolution, is aggregated and analysed at regional level to provide guidance on the expected temperature impact following specific LCC transitions.

Environmental impact assessment and eco-friendly decision-making in civil structures.

PubMed

Kim, Sang-Hyo; Choi, Moon-Seock; Mha, Ho-Seong; Joung, Jung-Yeun

2013-09-15

This study develops two useful procedures in performing an environmental-impact assessment. One is the advanced life-cycle assessment (LCA) method, which effectively tracks the flow of materials and considers the recycling and demolition of a civil structure. The other is an eco-friendly decision-making procedure, which may effectively apply when determining the prototype of a civil structure. The advanced LCA method differs from traditional LCA procedure, as it classifies the input material prior to the impact assessment. Classification work is performed to establish independent life-cycle stages for each material. The processes of recycling and demolition are appropriately added to the life-cycle stages. The impact assessment is performed separately for the materials, and results are aggregated at the end of the analysis. The eco-friendly decision-making procedure enables designers to choose an economical, and environmentally friendly, alternative during the planning phase of the construction project. This procedure rationally amalgamates economical value and environmental effects into a single indicator. The life cycle cost (LCC) of a structure can be analysed by using conventional LCC tools, whereas the environmental impact is estimated by LCA. The results from LCC and LCA are then integrated by using either a CO2 conversion method or an analytical hierarchy process (AHP). The CO2 conversion method presents the result as a monetary value, whereas the AHP presents the result as a non-dimensional value. A practical example using a steel box girder bridge and a pre-stressed concrete (PSC) box-girder bridge is also given in order to aid the understanding of the presented procedure. Copyright © 2013 Elsevier Ltd. All rights reserved.

Eco-efficiency for greenhouse gas emissions mitigation of municipal solid waste management: a case study of Tianjin, China.

PubMed

Zhao, Wei; Huppes, Gjalt; van der Voet, Ester

2011-06-01

The issue of municipal solid waste (MSW) management has been highlighted in China due to the continually increasing MSW volumes being generated and the limited capacity of waste treatment facilities. This article presents a quantitative eco-efficiency (E/E) analysis on MSW management in terms of greenhouse gas (GHG) mitigation. A methodology for E/E analysis has been proposed, with an emphasis on the consistent integration of life cycle assessment (LCA) and life cycle costing (LCC). The environmental and economic impacts derived from LCA and LCC have been normalized and defined as a quantitative E/E indicator. The proposed method was applied in a case study of Tianjin, China. The study assessed the current MSW management system, as well as a set of alternative scenarios, to investigate trade-offs between economy and GHG emissions mitigation. Additionally, contribution analysis was conducted on both LCA and LCC to identify key issues driving environmental and economic impacts. The results show that the current Tianjin's MSW management system emits the highest GHG and costs the least, whereas the situation reverses in the integrated scenario. The key issues identified by the contribution analysis show no linear relationship between the global warming impact and the cost impact in MSW management system. The landfill gas utilization scenario is indicated as a potential optimum scenario by the proposed E/E analysis, given the characteristics of MSW, technology levels, and chosen methodologies. The E/E analysis provides an attractive direction towards sustainable waste management, though some questions with respect to uncertainty need to be discussed further. Copyright © 2011 Elsevier Ltd. All rights reserved.

Up-scaling Stream Ecosystem Processes to Predict the Effects of Land Cover Change at a Watershed Scale in the Atlantic Tropical Rainforest.

NASA Astrophysics Data System (ADS)

Tromboni, F.; Feijó de Lima, R.; Silva-Júnior, E. F.; Lourenço-Amorim, C.; Zandoná, E.; Moulton, T. P.; Da Silva, B. S.; Silva-Araújo, M.; Thomas, S. A.

2015-12-01

Riparian land-cover change (LCC) causes a cascade of subsequent hierarchical effects that propagate through abiotic compartments until reaching the biota, altering stream ecosystem functioning. Due to the movement of water downstream, these lateral effects co-occur with longitudinal influences. We investigated both the lateral and longitudinal effects of deforestation in four streams in the Atlantic tropical rainforest of Brazil. We collected physical-chemical, geomorphic, hydrological data and samples of macroinvertebrates assemblages. We then categorized land cover at different scales (from different riparian and reach buffer sizes to sub and total watershed) using a SPOT-5 satellite image and ArcGIS. We also carried out a series of experiments along the streams to understand: 1) the mechanisms by which LCC affects periphyton and how these changes alter metabolism and nutrient uptake rates; 2) the downstream distance at which periphyton and the associated variables change in the transitions from one riparian category to the other. We used (i) a path analysis to test if our hypothesized land-cover cascade model described our data and (ii) non-linear models to describe the longitudinal effect on each variable. Our results showed that deforestation produced a range of physical changes at different spatial scale, longitudinally altering periphyton taxonomic composition (taxa depending on light), stoichiometry (nutritionally richer with increasing deforestation) and growth rates (greater in deforested). Macroinvertebrate assemblages behaved similarly to chlorophyll a in response to forest loss. Respiration rate increased with deforestation probably due to higher nutrient concentrations but primary production did not increase. Models were used to upscale LCC impacts on ecosystem processes from local scale experiments to landscape and our work has important implications for socio-economic decisions concerning ecosystem management and conservation.

Polytobacco, marijuana, and alcohol use patterns in college students: A latent class analysis.

PubMed

Haardörfer, Regine; Berg, Carla J; Lewis, Michael; Payne, Jackelyn; Pillai, Drishti; McDonald, Bennett; Windle, Michael

2016-08-01

Limited research has examined polysubstance use profiles among young adults focusing on the various tobacco products currently available. We examined use patterns of various tobacco products, marijuana, and alcohol using data from the baseline survey of a multiwave longitudinal study of 3418 students aged 18-25 recruited from seven U.S. college campuses. We assessed sociodemographics, individual-level factors (depression; perceptions of harm and addictiveness,), and sociocontextual factors (parental/friend use). We conducted a latent class analysis and multivariable logistic regression to examine correlates of class membership (Abstainers were referent group). Results indicated five classes: Abstainers (26.1% per past 4-month use), Alcohol only users (38.9%), Heavy polytobacco users (7.3%), Light polytobacco users (17.3%), and little cigar and cigarillo (LCC)/hookah/marijuana co-users (10.4%). The most stable was LCC/hookah/marijuana co-users (77.3% classified as such in past 30-day and 4-month timeframes), followed by Heavy polytobacco users (53.2% classified consistently). Relative to Abstainers, Heavy polytobacco users were less likely to be Black and have no friends using alcohol and perceived harm of tobacco and marijuana use lower. Light polytobacco users were older, more likely to have parents using tobacco, and less likely to have friends using tobacco. LCC/hookah/marijuana co-users were older and more likely to have parents using tobacco. Alcohol only users perceived tobacco and marijuana use to be less socially acceptable, were more likely to have parents using alcohol and friends using marijuana, but less likely to have friends using tobacco. These findings may inform substance use prevention and recovery programs by better characterizing polysubstance use patterns. Copyright © 2016 Elsevier Ltd. All rights reserved.

Polytobacco, marijuana, and alcohol use patterns in college students: A latent class analysis

PubMed Central

Haardörfer, Regine; Berg, Carla J.; Lewis, Michael; Payne, Jackelyn; Pillai, Drishti; McDonald, Bennett; Windle, Michael

2016-01-01

Limited research has examined polysubstance use profiles among young adults focusing on the various tobacco products currently available. We examined use patterns of various tobacco products, marijuana, and alcohol using data from the baseline survey of a multiwave longitudinal study of 3418 students aged 18-25 recruited from seven U.S. college campuses. We assessed sociodemographics, individual-level factors (depression; perceptions of harm and addictiveness,), and sociocontextual factors (parental/friend use). We conducted a latent class analysis and multivariable logistic regression to examine correlates of class membership (Abstainers were referent group). Results indicated five classes: Abstainers (26.1% per past 4-month use), Alcohol only users (38.9%), Heavy polytobacco users (7.3%), Light polytobacco users (17.3%), and little cigar and cigarillo (LCC)/hookah/marijuana co-users (10.4%). The most stable was LCC/hookah/marijuana co-users (77.3% classified as such in past 30-day and 4-month timeframes), followed by Heavy polytobacco users (53.2% classified consistently). Relative to Abstainers, Heavy polytobacco users were less likely to be Black and have no friends using alcohol and perceived harm of tobacco and marijuana use lower. Light polytobacco users were older, more likely to have parents using tobacco, and less likely to have friends using tobacco. LCC/hookah/marijuana co-users were older and more likely to have parents using tobacco. Alcohol only users perceived tobacco and marijuana use to be less socially acceptable, were more likely to have parents using alcohol and friends using marijuana, but less likely to have friends using tobacco. These findings may inform substance use prevention and recovery programs by better characterizing polysubstance use patterns. PMID:27074202

A multi-scale homogenization model for fine-grained porous viscoplastic polycrystals: I - Finite-strain theory

NASA Astrophysics Data System (ADS)

Song, Dawei; Ponte Castañeda, P.

2018-06-01

We make use of the recently developed iterated second-order homogenization method to obtain finite-strain constitutive models for the macroscopic response of porous polycrystals consisting of large pores randomly distributed in a fine-grained polycrystalline matrix. The porous polycrystal is modeled as a three-scale composite, where the grains are described by single-crystal viscoplasticity and the pores are assumed to be large compared to the grain size. The method makes use of a linear comparison composite (LCC) with the same substructure as the actual nonlinear composite, but whose local properties are chosen optimally via a suitably designed variational statement. In turn, the effective properties of the resulting three-scale LCC are determined by means of a sequential homogenization procedure, utilizing the self-consistent estimates for the effective behavior of the polycrystalline matrix, and the Willis estimates for the effective behavior of the porous composite. The iterated homogenization procedure allows for a more accurate characterization of the properties of the matrix by means of a finer "discretization" of the properties of the LCC to obtain improved estimates, especially at low porosities, high nonlinearties and high triaxialities. In addition, consistent homogenization estimates for the average strain rate and spin fields in the pores and grains are used to develop evolution laws for the substructural variables, including the porosity, pore shape and orientation, as well as the "crystallographic" and "morphological" textures of the underlying matrix. In Part II of this work has appeared in Song and Ponte Castañeda (2018b), the model will be used to generate estimates for both the instantaneous effective response and the evolution of the microstructure for porous FCC and HCP polycrystals under various loading conditions.

Transit bus life cycle cost and year 2007 emissions estimation.

DOT National Transportation Integrated Search

2007-06-01

The report presents a study of transit bus life cycle cost (LCC) analysis, and projected transit bus emissions and fuel economy for 2007 : model year buses. It covers four bus types: diesel buses using ultra low sulfur diesel (ULSD), diesel buses usi...

Enhancing the biodegradation of oil in sandy sediments with choline: a naturally methylated nitrogen compound.

PubMed

Mortazavi, Behzad; Horel, Agota; Anders, Jennifer S; Mirjafari, Arsalan; Beazley, Melanie J; Sobecky, Patricia A

2013-11-01

We investigated how additions of choline, a naturally occurring methylated nitrogen-containing compound, accelerated hydrocarbon degradation in sandy sediments contaminated with moderately weathered crude oil (4000 mg kg(-1) sediment). Addition of lauroylcholine chloride (LCC) and tricholine citrate (TCC) to oil contaminated sediments resulted in 1.6 times higher hydrocarbon degradation rates compared to treatments without added choline derivatives. However, the degradation rate constant for the oil contaminated sediments amended with LCC was similar to that in contaminated sediments amended with inorganic nitrogen, phosphorus, and glucose. Additions of LLC and TCC to sediments containing extensively weathered oil also resulted in enhanced mineralization rates. Cultivation-free 16S rRNA analysis revealed the presence of an extant microbial community with clones closely related to known hydrocarbon degraders from the Gammaproteobacteria, Alphaproteobacteria, and Firmicutes phyla. The results demonstrate that the addition of minimal amounts of organic compounds to oil contaminated sediments enhances the degradation of hydrocarbons. Copyright © 2013 Elsevier Ltd. All rights reserved.

Strategy on energy saving reconstruction of distribution networks based on life cycle cost

NASA Astrophysics Data System (ADS)

Chen, Xiaofei; Qiu, Zejing; Xu, Zhaoyang; Xiao, Chupeng

2017-08-01

Because the actual distribution network reconstruction project funds are often limited, the cost-benefit model and the decision-making method are crucial for distribution network energy saving reconstruction project. From the perspective of life cycle cost (LCC), firstly the research life cycle is determined for the energy saving reconstruction of distribution networks with multi-devices. Then, a new life cycle cost-benefit model for energy-saving reconstruction of distribution network is developed, in which the modification schemes include distribution transformers replacement, lines replacement and reactive power compensation. In the operation loss cost and maintenance cost area, the operation cost model considering the influence of load season characteristics and the maintenance cost segmental model of transformers are proposed. Finally, aiming at the highest energy saving profit per LCC, a decision-making method is developed while considering financial and technical constraints as well. The model and method are applied to a real distribution network reconstruction, and the results prove that the model and method are effective.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hart, Philip R.; Athalye, Rahul A.; Xie, YuLong

Moving to the ASHRAE Standard 90.1-2013 (ASHRAE 2013) edition from Standard 90.1-2010 (ASHRAE 2010) is cost-effective for the State of Arizona. The table below shows the state-wide economic impact of upgrading to Standard 90.1-2013 in terms of the annual energy cost savings in dollars per square foot, additional construction cost per square foot required by the upgrade, and life-cycle cost (LCC) per square foot. These results are weighted averages for all building types in all climate zones in the state, based on weightings shown in Table 4. The methodology used for this analysis is consistent with the methodology used inmore » the national cost-effectiveness analysis. Additional results and details on the methodology are presented in the following sections. The report provides analysis of two LCC scenarios: Scenario 1, representing publicly-owned buildings, considers initial costs, energy costs, maintenance costs, and replacement costs—without borrowing or taxes. Scenario 2, representing privately-owned buildings, adds borrowing costs and tax impacts.« less

Self-healing Li-Al layered double hydroxide conversion coating modified with aspartic acid for 6N01 Al alloy

NASA Astrophysics Data System (ADS)

Zhang, Caixia; Luo, Xiaohu; Pan, Xinyu; Liao, Liying; Wu, Xiaosong; Liu, Yali

2017-02-01

A self-healing Li-Al layered double hydroxide conversion coating (LCC) modified with aspartic acid (ALCC) was prepared on 6N01 Al alloy for corrosion protection. Scanning electron microscopy (SEM) showed that a compact thin film has been successfully formed on the alloy. X-ray diffraction (XRD) and FT-IR spectra proved that species of aspartic acid anions were successfully intercalated into LCC. Potentiodynamic polarization, electrochemical impedance spectroscopy (EIS) and neutral salt spray (NSS) testing showed that the resultant ALCC could provide effective corrosion protection for the Al alloy. During immersion of the ALCC-coated alloy in 3.5% NaCl solution, new film was formed in the area of artificially introduced scratch, indicating its self-healing capability. XPS results demonstrated that Cl- anions exchange partial Asp anions according to the change content of element on conversion coating. From the above results, the possible mechanism via exchange/self-assembly was proposed to illustrate the phenomenon of self-healing.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hart, Philip R.; Athalye, Rahul A.; Xie, YuLong

Moving to the ASHRAE Standard 90.1-2013 (ASHRAE 2013) edition from Standard 90.1-2010 (ASHRAE 2010) is cost-effective for the State of Hawaii. The table below shows the state-wide economic impact of upgrading to Standard 90.1-2013 in terms of the annual energy cost savings in dollars per square foot, additional construction cost per square foot required by the upgrade, and life-cycle cost (LCC) per square foot. These results are weighted averages for all building types in all climate zones in the state, based on weightings shown in Table 4. The methodology used for this analysis is consistent with the methodology used inmore » the national cost-effectiveness analysis. Additional results and details on the methodology are presented in the following sections. The report provides analysis of two LCC scenarios: Scenario 1, representing publicly-owned buildings, considers initial costs, energy costs, maintenance costs, and replacement costs—without borrowing or taxes. Scenario 2, representing privately-owned buildings, adds borrowing costs and tax impacts.« less

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hart, Philip R.; Athalye, Rahul A.; Xie, YuLong

Moving to the ASHRAE Standard 90.1-2013 (ASHRAE 2013) edition from Standard 90.1-2010 (ASHRAE 2010) is cost-effective for the State of Connecticut. The table below shows the state-wide economic impact of upgrading to Standard 90.1-2013 in terms of the annual energy cost savings in dollars per square foot, additional construction cost per square foot required by the upgrade, and life-cycle cost (LCC) per square foot. These results are weighted averages for all building types in all climate zones in the state, based on weightings shown in Table 4. The methodology used for this analysis is consistent with the methodology used inmore » the national cost-effectiveness analysis. Additional results and details on the methodology are presented in the following sections. The report provides analysis of two LCC scenarios: Scenario 1, representing publicly-owned buildings, considers initial costs, energy costs, maintenance costs, and replacement costs—without borrowing or taxes. Scenario 2, representing privately-owned buildings, adds borrowing costs and tax impacts.« less

Economic and environmental sustainability of submerged anaerobic MBR-based (AnMBR-based) technology as compared to aerobic-based technologies for moderate-/high-loaded urban wastewater treatment.

PubMed

Pretel, R; Robles, A; Ruano, M V; Seco, A; Ferrer, J

2016-01-15

The objective of this study was to assess the economic and environmental sustainability of submerged anaerobic membrane bioreactors (AnMBRs) in comparison with aerobic-based technologies for moderate-/high-loaded urban wastewater (UWW) treatment. To this aim, a combined approach of steady-state performance modelling, life cycle analysis (LCA) and life cycle costing (LCC) was used, in which AnMBR (coupled with an aerobic-based post-treatment) was compared to aerobic membrane bioreactor (AeMBR) and conventional activated sludge (CAS). AnMBR with CAS-based post-treatment for nutrient removal was identified as a sustainable option for moderate-/high-loaded UWW treatment: low energy consumption and reduced sludge production could be obtained at given operating conditions. In addition, significant reductions can be achieved in different aspects of environmental impact (global warming potential (GWP), abiotic depletion, acidification, etc.) and LCC over existing UWW treatment technologies. Copyright © 2015 Elsevier Ltd. All rights reserved.

Life-cycle cost as basis to optimize waste collection in space and time: A methodology for obtaining a detailed cost breakdown structure.

PubMed

Sousa, Vitor; Dias-Ferreira, Celia; Vaz, João M; Meireles, Inês

2018-05-01

Extensive research has been carried out on waste collection costs mainly to differentiate costs of distinct waste streams and spatial optimization of waste collection services (e.g. routes, number, and location of waste facilities). However, waste collection managers also face the challenge of optimizing assets in time, for instance deciding when to replace and how to maintain, or which technological solution to adopt. These issues require a more detailed knowledge about the waste collection services' cost breakdown structure. The present research adjusts the methodology for buildings' life-cycle cost (LCC) analysis, detailed in the ISO 15686-5:2008, to the waste collection assets. The proposed methodology is then applied to the waste collection assets owned and operated by a real municipality in Portugal (Cascais Ambiente - EMAC). The goal is to highlight the potential of the LCC tool in providing a baseline for time optimization of the waste collection service and assets, namely assisting on decisions regarding equipment operation and replacement.

75 FR 26299 - Self-Regulatory Organizations; Notice of Filing and Immediate Effectiveness of Proposed Rule...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-11

... Kinross Gold Corp. 273 EP El Paso Corp. 274 SEED Origin Agritech Ltd. 275 WIN Windstream Corp. 279 DHI DR Horton Inc. 280 ADBE Adobe Systems Inc. 281 PCX Patriot Coal Corp. 282 SPWRA SunPower Corp. 284 LCC US...

Infusing Technology into Education.

ERIC Educational Resources Information Center

Bowen, Raymond C.

At LaGuardia Community College (LCC), technology has become central to the college curriculum, and has enriched the college's cooperative and secondary education programs. In addition to employing technology to accommodate various teaching/learning styles at the college, LaGuardia provides students with as many opportunities as possible to use…

Flavors and Risk: Perceptions of Flavors in Little Cigars and Cigarillos Among U.S. Adults, 2015.

PubMed

Nyman, Amy L; Sterling, Kymberle L; Majeed, Ban A; Jones, Dina M; Eriksen, Michael P

2017-06-28

Flavored little cigars, cigarillos, and filtered cigars (LCCs) are popular and pose unique health risks. This study explored risk perceptions of flavors in LCCs and the relationship between perceptions and use among U.S. adults. Data were from the 2015 Tobacco Products and Risk Perceptions Survey of a national probability sample of 6,051 adults, conducted online, August-September, 2015. The analytic sample consisted of 5,105 adults aware of LCCs and 2,174 who had ever used any type of LCCs. Just over half of adults reported flavors in LCCs as "very" or "somewhat" risky, while more than one-third of adults reported they didn't know the risks of flavors in LCCs. Younger adults, males, and users of any LCCs were more likely than older adults, females and non-users, respectively, to perceive LCC flavors as less risky. Those who perceived LCC flavors as "not at all risky" or "a little risky" were roughly twice as likely to have ever used flavored LCCs compared to those who reported not knowing the risks (AOR=2.07, 95% CI=1.16, 3.69 and AOR=1.96, 95% CI=1.26, 3.06). Those who reported LCC flavors as "very risky" were also more likely to have ever used flavored LCCs than those who reported not knowing the risks (AOR=1.50, 95% CI=1.13, 1.98). Though the proportion of adults assigning low risk to flavors in LCCs is small, these adults are more likely to use flavored LCCs. The association of use with risk perceptions of flavors in LCCs should be addressed in health risk campaigns. Flavored LCCs are popular, particularly among young adults. While understanding the impact of flavors is an FDA research priority, little is currently known about perceptions of risk associated with flavors in LCCs. This study explores the nature of risk perceptions of flavors in LCCs, and the relationship between perceptions and flavored LCC use. We conclude that perception of risk of flavors in LCCs is related to use of these products, particularly perception of less risk. Considering the relationship between use and risk perceptions would be helpful in constructing health risk messaging. © The Author 2017. Published by Oxford University Press on behalf of the Society for Research on Nicotine and Tobacco. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

An Assessment of Cost Improvements in the NASA COTS - CRS Program and Implications for Future NASA Missions

NASA Technical Reports Server (NTRS)

Zapata, Edgar

2017-01-01

This review brings rigorous life cycle cost (LCC) analysis into discussions about COTS program costs. We gather publicly available cost data, review the data for credibility, check for consistency among sources, and rigorously define and analyze specific cost metrics.

Developing a Metric for the Cost of Green House Gas Abatement

DOT National Transportation Integrated Search

2017-02-28

The authors introduce the levelized cost of carbon (LCC), a metric that can be used to evaluate MassDOT CO2 abatement projects in terms of their cost-effectiveness. The study presents ways in which the metric can be used to rank projects. The data ar...

PREDICTING CHEMICAL REACTIVITY OF HUMIC SUBSTANCES FOR MINERALS AND XENOBIOTICS: USE OF COMPUTATIONAL CHEMISTRY, SCANNING PROBE MICROSCOPY AND VIRTUAL REALITY

EPA Science Inventory

In this chapter we review the literature on scanning probe microscopy (SPM), virtual reality (VR), and computational chemistry and our earlier work dealing with modeling lignin, lignin-carbohydrate complexes (LCC), humic substances (HSs) and non-bonded organo-mineral interactions...

Effect of menstrual cycle phase on corticolimbic brain activation by visual food cues.

PubMed

Frank, Tamar C; Kim, Ginah L; Krzemien, Alicja; Van Vugt, Dean A

2010-12-02

Food intake is decreased during the late follicular phase and increased in the luteal phase of the menstrual cycle. While a changing ovarian steroid milieu is believed to be responsible for this behavior, the specific mechanisms involved are poorly understood. Brain activity in response to visual food stimuli was compared during the estrogen dominant peri-ovulatory phase and the progesterone dominant luteal phase of the menstrual cycle. Twelve women underwent functional magnetic resonance imaging during the peri-ovulatory and luteal phases of the menstrual cycle in a counterbalanced fashion. Whole brain T2* images were collected while subjects viewed pictures of high calorie (HC) foods, low calorie (LC) foods, and control (C) pictures presented in a block design. Blood oxygen level dependent (BOLD) signal in the late follicular phase and luteal phase was determined for the contrasts HC-C, LC-C, HC-LC, and LC-HC. Both HC and LC stimuli activated numerous corticolimbic brain regions in the follicular phase, whereas only HC stimuli were effective in the luteal phase. Activation of the nucleus accumbens (NAc), amygdala, and hippocampus in response to the HC-C contrast and the hippocampus in response to the LC-C contrast was significantly increased in the late follicular phase compared to the luteal phase. Activation of the orbitofrontal cortex and mid cingulum in response to the HC-LC contrast was greater during the luteal phase. These results demonstrate for the first time that brain responses to visual food cues are influenced by menstrual cycle phase. We postulate that ovarian steroid modulation of the corticolimbic brain contributes to changes in ingestive behavior during the menstrual cycle. Copyright © 2010 Elsevier B.V. All rights reserved.

Inhibition of mTOR's Catalytic Site by PKI-587 Is a Promising Therapeutic Option for Gastroenteropancreatic Neuroendocrine Tumor Disease

PubMed Central

Freitag, Helma; Christen, Friederike; Lewens, Florentine; Grass, Irina; Briest, Franziska; Iwaszkiewicz, Sara; Siegmund, Britta; Grabowski, Patricia

2017-01-01

Background The characteristic clinical heterogeneity and mostly slow-growing behavior of gastroenteropancreatic neuroendocrine neoplasms (GEP-NENs) cause problems in finding appropriate treatments. Thus, the current therapy options are not satisfactory. PKI-587 is a highly potent, novel dual inhibitor of PI3K and mTORC1/C2. Aim We assessed the effects of PKI-587 in different GEP-NEN tumor models, including the poorly differentiated cell line LCC-18, and compared them with those of the established mTORC1 inhibitor everolimus. Methods We treated BON, QGP-1, KRJ-I, and LCC-18 cell lines with increasing concentrations of the inhibitor PKI-587, and compared the results with those of everolimus and DMSO. We assessed the impact of the treatments on viability (WST-1 assay), on apoptotic processes (caspase 3/7 assay, JC-1), and on cell cycle regulation (flow cytometry). We determined alterations in signaling mediators by phosphor-specific Western blot analysis and conducted multiplexed gene expression analysis (nCounter® technology). Results In all cell lines, PKI-587 dose-dependently inhibited proliferation, whereas everolimus was less effective. Treatment with PKI-587 led to cell cycle arrest and induction of apoptosis and successfully suppressed activity of the direct mTORC1 target 4E-BP1, a crucial factor for tumor genesis only partially inhibited by everolimus. Gene expression analyses revealed relevant changes of RAS, MAPK, STAT, and PI3K pathway genes after treatment. Treatment-dependent and cell line-characteristic effects on AKT/Rb/E2F signaling regarding cell cycle control and apoptosis are extensively discussed in this paper. Conclusion PI3K/mTOR dual targeting is a promising new therapeutic approach in neuroendocrine tumor disease that should be evaluated in further clinical trials. PMID:27513674

NASA Weather Support 2017

NASA Technical Reports Server (NTRS)

Carroll, Matt

2017-01-01

In the mid to late 1980's, as NASA was studying ways to improve weather forecasting capabilities to reduce excessive weather launch delays and to reduce excessive weather Launch Commit Criteria (LCC) waivers, the Challenger Accident occurred and the AC-67 Mishap occurred.[1] NASA and USAF weather personnel had advance knowledge of extremely high levels of weather hazards that ultimately caused or contributed to both of these accidents. In both cases, key knowledge of the risks posed by violations of weather LCC was not in the possession of final decision makers on the launch teams. In addition to convening the mishap boards for these two lost missions, NASA convened expert meteorological boards focusing on weather support. These meteorological boards recommended the development of a dedicated organization with the highest levels of weather expertise and influence to support all of American spaceflight. NASA immediately established the Weather Support Office (WSO) in the Office of Space Flight (OSF), and in coordination with the United Stated Air Force (USAF), initiated an overhaul of the organization and an improvement in technology used for weather support as recommended. Soon after, the USAF established a senior civilian Launch Weather Officer (LWO) position to provide meteorological support and continuity of weather expertise and knowledge over time. The Applied Meteorology Unit (AMU) was established by NASA, USAF, and the National Weather Service to support initiatives to place new tools and methods into an operational status. At the end of the Shuttle Program, after several weather office reorganizations, the WSO function had been assigned to a weather branch at Kennedy Space Center (KSC). This branch was dismantled in steps due to further reorganization, loss of key personnel, and loss of budget line authority. NASA is facing the loss of sufficient expertise and leadership required to provide current levels of weather support. The recommendation proposed herein is to re-establish the WSO under a high level office, with funding set at about the same levels as today, with a revitalized charter and focus to allow for the WSO to operate as originally intended.

EDOS operations concept and development approach

NASA Technical Reports Server (NTRS)

Knoble, G.; Garman, C.; Alcott, G.; Ramchandani, C.; Silvers, J.

1994-01-01

The Earth Observing System (EOS) Data and Operations System (EDOS) is being developed by the National Aeronautics and Space Administration (NASA) Goddard Space Flight Center (GSFC) for the capture, level zero processing, distribution, and backup archiving of high speed telemetry data received from EOS spacecraft. All data received will conform to the Consultative Committee for Space Data Standards (CCSDS) recommendations. The major EDOS goals are to: (1) minimize EOS program costs to implement and operate EDOS; (2) respond effectively to EOS growth requirements; and (3) maintain compatibility with existing and enhanced versions of NASA institutional systems required to support EOS spacecraft. In order to meet these goals, the following objectives have been defined for EDOS: (1) standardize EDOS interfaces to maximize utility for future requirements; (2) emphasize life-cycle cost (LCC) considerations (rather than procurement costs) in making design decisions and meeting reliability, maintainability, availability (RMA) and upgradability requirements; (3) implement data-driven operations to the maximum extent possible to minimize staffing requirements and to maximize system responsiveness; (4) provide a system capable of simultaneously supporting multiple spacecraft, each in different phases of their life-cycles; (5) provide for technology insertion features to accommodate growth and future LCC reductions during the operations phase; and (6) provide a system that is sufficiently robust to accommodate incremental performance upgrades while supporting operations. Operations concept working group meetings were facilitated to help develop the EDOS operations concept. This provided a cohesive concept that met with approval of responsible personnel from the start. This approach not only speeded up the development process by reducing review cycles, it also provided a medium for generating good ideas that were immediately molded into feasible concepts. The operations concept was then used as a basis for the EDOS specification. When it was felt that concept elements did not support detailed requirements, the facilitator process was used to resolve discrepancies or to add new concept elements to support the specification. This method provided an ongoing revisal of the operations concept and prevented large revisions at the end of the requirement analysis phase of system development.

Navigating the high seas of Federal Programs to ensure usable science delivery

NASA Astrophysics Data System (ADS)

Bachelet, D. M.; Gough, M.; Baker, B.; Sheehan, T.; Mutch, T.; Brown, M.

2016-12-01

Conservation Biology Institute (CBI) has been developing web applications to serve credible and usable information that allows land stewards, as well as the general public, to better understand the challenges posed by on-going environmental change. Working with 18 Landscape Conservation Cooperatives (LCCs), CBI has developed Conservation Atlases that allow users to visualize regional spatial data. Some include specific tools such as the Conservation Blueprint in the South Atlantic LCC conservation atlas which is a living spatial plan that identifies priority areas for shared conservation action based on ecosystem indicator condition and connectivity. More than 400 people from over 100 different organizations have actively participated in its development so far. CBI worked closely with 4 LCCs to design a Landscape Climate Dashboard (http://bit.ly/2atu8Df) that provides CMIP5 climate projections averaged over federally and tribally protected lands in the western US. With support from BLM and Great Basin LCC, a Sagebrush Climate Console provides managers short-term NOAA forecasts, CMIP5 climate projections, regional intactness and site sensitivity over sagebrush extent and grouse range. The USDA Pacific Northwest Climate Hub and the US Forest Service are currently funding a collaborative effort between CBI programmers, USFS and Oregon State University forest geneticists to develop a seedlot source tool for the conterminous US and Alaska. This tool has inspired Great Basin sagebrush restoration scientists who have collected information on seed provenance and will hopefully soon collaborate to help develop the first seed source tool for sagebrush managers. Furthermore, with support from USDA Northwest Climate Hub, CBI's Data Basin site (http://bit.ly/2aLRlhZ) is providing downscaled CMIP5 climate projections and impacts from a Climate Science Center and NOAA Regional Integrated Science Assessment funded project that was the result of a collaborative effort between Oregon State University, University of Idaho, and the University of Washington. With much experience with local and regional managers and federal programs we will show examples of exciting successes and report on some challenges in coordinating exchanges and extracting valuable feedback to improve the usability of web applications.

78 FR 13563 - Energy Conservation Program: Availability of the Preliminary Technical Support Document for...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-28

... consideration: (1) Engineering, (2) energy-use characterization, (3) product price determination, (4) LCC and... use by incorporating projected energy prices and installed stock in each year. DOE calculates annual... DEPARTMENT OF ENERGY 10 CFR Part 430 [Docket No. EERE-2011-BT-STD-0006] RIN 1904-AC43 Energy...

A Blueprint for Excellence: Lakeland Community College.

ERIC Educational Resources Information Center

Lakeland Community Coll., Mentor, OH.

In 1988, Lakeland Community College (LCC), in Mentor, Ohio, began a strategic planning process to refocus the college on its mission, identify priorities for the years ahead, and establish a process to achieve them. Five strategic priorities were identified: (1) sustain and strengthen academic quality for students by adding at least 25 full-time…

Regional Training Needs Assessment: Dual Perspectives Eliminate the Guessing Game.

ERIC Educational Resources Information Center

Goodkind, Hilary M.

To better determine the educational and training needs of local employees, Lane Community College (LCC), in Oregon, undertook a project to analyze responses from a statewide survey of employers' perceptions of training needs and conduct focus groups with Lane County employees regarding their perceptions. The statewide study surveyed 6,010…

The land-cover cascade: relationships coupling land and water

Treesearch

C.L. Burcher; H.M. Valett; E.F. Benfield

2007-01-01

We introduce the land-cover cascade (LCC) as a conceptual framework to quantify the transfer of land-cover-disturbance effects to stream biota. We hypothesize that disturbance is propagated through multivariate systems through key variables that transform a disturbance and pass a reorganized disturbance effect to the next hierarchical level where the process repeats...

Follow-Up of 1974 Graduates in Selected Technology Programs, Lakeland Community College.

ERIC Educational Resources Information Center

Lakeland Community Coll., Mentor, OH.

In order to fulfill the requirements of the Ohio State Department of Vocational Education, Lakeland Community College (LCC) conducted a telephone followup survey to determine the employment activities of students who graduated from selected technology programs in 1974. Information regarding type of employment, job titles, and salaries was sought.…

Cost Effectiveness of ASHRAE Standard 90.1-2013 for the State of Texas

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hart, Philip R.; Athalye, Rahul A.; Xie, YuLong

Moving to the ASHRAE Standard 90.1-2013 (ASHRAE 2013) edition from Standard 90.1-2010 (ASHRAE 2010) is cost-effective for the State of Texas. The table below shows the state-wide economic impact of upgrading to Standard 90.1-2013 in terms of the annual energy cost savings in dollars per square foot, additional construction cost per square foot required by the upgrade, and life-cycle cost (LCC) per square foot. These results are weighted averages for all building types in all climate zones in the state, based on weightings shown in Table 4. The methodology used for this analysis is consistent with the methodology used inmore » the national cost-effectiveness analysis. Additional results and details on the methodology are presented in the following sections. The report provides analysis of two LCC scenarios: Scenario 1, representing publicly-owned buildings, considers initial costs, energy costs, maintenance costs, and replacement costs—without borrowing or taxes. Scenario 2, representing privately-owned buildings, adds borrowing costs and tax impacts.« less

Origin of the DUPAL anomaly in mantle xenoliths of Patagonia (Argentina) and geodynamic consequences

NASA Astrophysics Data System (ADS)

Mazzucchelli, Maurizio; Cipriani, Anna; Hémond, Christophe; Zanetti, Alberto; Bertotto, Gustavo Walter; Cingolani, Carlos Alberto

2016-04-01

The sub-continental lithospheric mantle of South America has been known for some time to carry the DUPAL isotope anomaly as seen in volcanics from the Paraná volcanic province. However, this has not allowed discriminating whether the DUPAL anomaly is a primary feature of the mantle source or acquired during the upwelling and emplacement of the primary magmas. We discovered mantle xenoliths from the Tres Lagos location in Patagonia that carry evidence of percolation by metasomatic melts that imparted the DUPAL isotope anomaly signature. We discuss a model that requires four isotope components (LCC, EM2, HIMU and DM) to account for the Sr, Nd and Pb isotope variability of our samples. We propose that upwelling of hot astenosphere during the Miocene could have triggered the melting of the LCC and EM2 components carrying the DUPAL anomaly, previously entrained in the subcontinental mantle by subduction. These ascending melts would have then metasomatised the local SCLM characterised by DMM and HIMU geochemical affinity generating the hybrid DUPAL-bearing mantle sampled by the Tres Lagos xenoliths.

Cost Effectiveness of ASHRAE Standard 90.1-2013 for the State of Minnesota

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hart, Philip R.; Athalye, Rahul A.; Xie, YuLong

2015-12-01

Moving to the ASHRAE Standard 90.1-2013 (ASHRAE 2013) edition from Standard 90.1-2010 (ASHRAE 2010) is cost-effective for the State of Minnesota. The table below shows the state-wide economic impact of upgrading to Standard 90.1-2013 in terms of the annual energy cost savings in dollars per square foot, additional construction cost per square foot required by the upgrade, and life-cycle cost (LCC) per square foot. These results are weighted averages for all building types in all climate zones in the state, based on weightings shown in Table 4. The methodology used for this analysis is consistent with the methodology used inmore » the national cost-effectiveness analysis. Additional results and details on the methodology are presented in the following sections. The report provides analysis of two LCC scenarios: Scenario 1, representing publicly-owned buildings, considers initial costs, energy costs, maintenance costs, and replacement costs—without borrowing or taxes. Scenario 2, representing privately-owned buildings, adds borrowing costs and tax impacts.« less

Cost Effectiveness of ASHRAE Standard 90.1-2013 for the State of Indiana

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hart, Philip R.; Athalye, Rahul A.; Xie, YuLong

Moving to the ASHRAE Standard 90.1-2013 (ASHRAE 2013) edition from Standard 90.1-2010 (ASHRAE 2010) is cost-effective for the State of Indiana. The table below shows the state-wide economic impact of upgrading to Standard 90.1-2013 in terms of the annual energy cost savings in dollars per square foot, additional construction cost per square foot required by the upgrade, and life-cycle cost (LCC) per square foot. These results are weighted averages for all building types in all climate zones in the state, based on weightings shown in Table 4. The methodology used for this analysis is consistent with the methodology used inmore » the national cost-effectiveness analysis. Additional results and details on the methodology are presented in the following sections. The report provides analysis of two LCC scenarios: Scenario 1, representing publicly-owned buildings, considers initial costs, energy costs, maintenance costs, and replacement costs—without borrowing or taxes. Scenario 2, representing privately-owned buildings, adds borrowing costs and tax impacts.« less

A 55-year-old female with leukoencephalopathy with cerebral calcifications and cysts: Case report and radiopathologic description.

PubMed

Novo, Jorge; Lin, Diana; Shanks, Megan; Kocak, Mehmet; Arvanitis, Leonidas

2017-11-01

Adult-onset leukoencephalopathies with increased cerebral volume can present a potentially challenging diagnosis for the pathologist. We present the case of a patient with a rare adult-onset disease called Leukoencephalopathy with cerebral Calcifications and Cysts (LCC). A 55-year-old woman with a history of morning headaches, mild memory loss, diabetes, and hypertension presented to the emergency department with acute onset altered mental status. CT scan revealed multiple small hypodense lesions in the white matter with calcifications in the bilateral cerebral hemispheres, basal ganglia, pons, and cerebellar hemispheres. MRI showed multiple complex/hemorrhagic cystic lesions with partial enhancement in addition to calcifications bilaterally in the frontotemporal white matter, pons, and cerebellar hemispheres, and diffuse white matter signal abnormality. The differential diagnosis included chronic infection, chronic thromboembolic disease, and neoplasm. The biopsy revealed extensive geode-like mineralization as well as smaller calcifications (calcospherites) with associated sclerosis, Rosenthal fibers, angiomatous proliferation of blood vessels with thrombosis and microbleeds. We discuss the differential diagnosis, radiologic and detailed histologic features of LCC. Copyright © 2017 Elsevier GmbH. All rights reserved.

Robust 3D face landmark localization based on local coordinate coding.

PubMed

Song, Mingli; Tao, Dacheng; Sun, Shengpeng; Chen, Chun; Maybank, Stephen J

2014-12-01

In the 3D facial animation and synthesis community, input faces are usually required to be labeled by a set of landmarks for parameterization. Because of the variations in pose, expression and resolution, automatic 3D face landmark localization remains a challenge. In this paper, a novel landmark localization approach is presented. The approach is based on local coordinate coding (LCC) and consists of two stages. In the first stage, we perform nose detection, relying on the fact that the nose shape is usually invariant under the variations in the pose, expression, and resolution. Then, we use the iterative closest points algorithm to find a 3D affine transformation that aligns the input face to a reference face. In the second stage, we perform resampling to build correspondences between the input 3D face and the training faces. Then, an LCC-based localization algorithm is proposed to obtain the positions of the landmarks in the input face. Experimental results show that the proposed method is comparable to state of the art methods in terms of its robustness, flexibility, and accuracy.

Cost Effectiveness of ASHRAE Standard 90.1-2013 for the State of Florida

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hart, Philip R.; Athalye, Rahul A.; Xie, YuLong

2015-12-01

Moving to the ASHRAE Standard 90.1-2013 (ASHRAE 2013) edition from Standard 90.1-2010 (ASHRAE 2010) is cost-effective for the State of Florida. The table below shows the state-wide economic impact of upgrading to Standard 90.1-2013 in terms of the annual energy cost savings in dollars per square foot, additional construction cost per square foot required by the upgrade, and life-cycle cost (LCC) per square foot. These results are weighted averages for all building types in all climate zones in the state, based on weightings shown in Table 4. The methodology used for this analysis is consistent with the methodology used inmore » the national cost-effectiveness analysis. Additional results and details on the methodology are presented in the following sections. The report provides analysis of two LCC scenarios: Scenario 1, representing publicly-owned buildings, considers initial costs, energy costs, maintenance costs, and replacement costs—without borrowing or taxes. Scenario 2, representing privately-owned buildings, adds borrowing costs and tax impacts.« less

Cost Effectiveness of ASHRAE Standard 90.1-2013 for the State of Maine

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hart, Philip R.; Athalye, Rahul A.; Xie, YuLong

2015-12-01

Moving to the ASHRAE Standard 90.1-2013 (ASHRAE 2013) edition from Standard 90.1-2010 (ASHRAE 2010) is cost-effective for the State of Maine. The table below shows the state-wide economic impact of upgrading to Standard 90.1-2013 in terms of the annual energy cost savings in dollars per square foot, additional construction cost per square foot required by the upgrade, and life-cycle cost (LCC) per square foot. These results are weighted averages for all building types in all climate zones in the state, based on weightings shown in Table 4. The methodology used for this analysis is consistent with the methodology used inmore » the national cost-effectiveness analysis. Additional results and details on the methodology are presented in the following sections. The report provides analysis of two LCC scenarios: Scenario 1, representing publicly-owned buildings, considers initial costs, energy costs, maintenance costs, and replacement costs—without borrowing or taxes. Scenario 2, representing privately-owned buildings, adds borrowing costs and tax impacts.« less

Cost Effectiveness of ASHRAE Standard 90.1-2013 for the State of Vermont

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hart, Philip R.; Athalye, Rahul A.; Xie, YuLong

2015-12-01

Moving to the ASHRAE Standard 90.1-2013 (ASHRAE 2013) edition from Standard 90.1-2010 (ASHRAE 2010) is cost-effective for the State of Vermont. The table below shows the state-wide economic impact of upgrading to Standard 90.1-2013 in terms of the annual energy cost savings in dollars per square foot, additional construction cost per square foot required by the upgrade, and life-cycle cost (LCC) per square foot. These results are weighted averages for all building types in all climate zones in the state, based on weightings shown in Table 4. The methodology used for this analysis is consistent with the methodology used inmore » the national cost-effectiveness analysis. Additional results and details on the methodology are presented in the following sections. The report provides analysis of two LCC scenarios: Scenario 1, representing publicly-owned buildings, considers initial costs, energy costs, maintenance costs, and replacement costs—without borrowing or taxes. Scenario 2, representing privately-owned buildings, adds borrowing costs and tax impacts.« less

Cost Effectiveness of ASHRAE Standard 90.1-2013 for the State of Michigan

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hart, Philip R.; Athalye, Rahul A.; Xie, YuLong

Moving to the ASHRAE Standard 90.1-2013 (ASHRAE 2013) edition from Standard 90.1-2010 (ASHRAE 2010) is cost-effective for the State of Michigan. The table below shows the state-wide economic impact of upgrading to Standard 90.1-2013 in terms of the annual energy cost savings in dollars per square foot, additional construction cost per square foot required by the upgrade, and life-cycle cost (LCC) per square foot. These results are weighted averages for all building types in all climate zones in the state, based on weightings shown in Table 4. The methodology used for this analysis is consistent with the methodology used inmore » the national cost-effectiveness analysis. Additional results and details on the methodology are presented in the following sections. The report provides analysis of two LCC scenarios: Scenario 1, representing publicly-owned buildings, considers initial costs, energy costs, maintenance costs, and replacement costs—without borrowing or taxes. Scenario 2, representing privately-owned buildings, adds borrowing costs and tax impacts.« less

Cost Effectiveness of ASHRAE Standard 90.1-2013 for the State of Alabama

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hart, Philip R.; Athalye, Rahul A.; Xie, YuLong

2015-12-01

Moving to the ASHRAE Standard 90.1-2013 (ASHRAE 2013) edition from Standard 90.1-2010 (ASHRAE 2010) is cost-effective for the State of Alabama. The table below shows the state-wide economic impact of upgrading to Standard 90.1-2013 in terms of the annual energy cost savings in dollars per square foot, additional construction cost per square foot required by the upgrade, and life-cycle cost (LCC) per square foot. These results are weighted averages for all building types in all climate zones in the state, based on weightings shown in Table 4. The methodology used for this analysis is consistent with the methodology used inmore » the national cost-effectiveness analysis. Additional results and details on the methodology are presented in the following sections. The report provides analysis of two LCC scenarios: Scenario 1, representing publicly-owned buildings, considers initial costs, energy costs, maintenance costs, and replacement costs—without borrowing or taxes. Scenario 2, representing privately-owned buildings, adds borrowing costs and tax impacts.« less

Cost Effectiveness of ASHRAE Standard 90.1-2013 for the State of New Hampshire

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hart, Philip R.; Athalye, Rahul A.; Xie, YuLong

2015-12-01

Moving to the ASHRAE Standard 90.1-2013 (ASHRAE 2013) edition from Standard 90.1-2010 (ASHRAE 2010) is cost-effective for the State of New Hampshire. The table below shows the state-wide economic impact of upgrading to Standard 90.1-2013 in terms of the annual energy cost savings in dollars per square foot, additional construction cost per square foot required by the upgrade, and life-cycle cost (LCC) per square foot. These results are weighted averages for all building types in all climate zones in the state, based on weightings shown in Table 4. The methodology used for this analysis is consistent with the methodology usedmore » in the national cost-effectiveness analysis. Additional results and details on the methodology are presented in the following sections. The report provides analysis of two LCC scenarios: Scenario 1, representing publicly-owned buildings, considers initial costs, energy costs, maintenance costs, and replacement costs—without borrowing or taxes. Scenario 2, representing privately-owned buildings, adds borrowing costs and tax impacts.« less

Cost Effectiveness of ASHRAE Standard 90.1-2013 for the State of New Mexico

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hart, Philip R.; Athalye, Rahul A.; Xie, YuLong

2015-12-01

Moving to the ASHRAE Standard 90.1-2013 (ASHRAE 2013) edition from Standard 90.1-2010 (ASHRAE 2010) is cost-effective for the State of New Mexico. The table below shows the state-wide economic impact of upgrading to Standard 90.1-2013 in terms of the annual energy cost savings in dollars per square foot, additional construction cost per square foot required by the upgrade, and life-cycle cost (LCC) per square foot. These results are weighted averages for all building types in all climate zones in the state, based on weightings shown in Table 4. The methodology used for this analysis is consistent with the methodology usedmore » in the national cost-effectiveness analysis. Additional results and details on the methodology are presented in the following sections. The report provides analysis of two LCC scenarios: Scenario 1, representing publicly-owned buildings, considers initial costs, energy costs, maintenance costs, and replacement costs—without borrowing or taxes. Scenario 2, representing privately-owned buildings, adds borrowing costs and tax impacts.« less

Cost Effectiveness of ASHRAE Standard 90.1-2013 for the State of Colorado

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hart, Philip R.; Athalye, Rahul A.; Xie, YuLong

2015-12-01

Moving to the ASHRAE Standard 90.1-2013 (ASHRAE 2013) edition from Standard 90.1-2010 (ASHRAE 2010) is cost-effective for the State of Colorado. The table below shows the state-wide economic impact of upgrading to Standard 90.1-2013 in terms of the annual energy cost savings in dollars per square foot, additional construction cost per square foot required by the upgrade, and life-cycle cost (LCC) per square foot. These results are weighted averages for all building types in all climate zones in the state, based on weightings shown in Table 4. The methodology used for this analysis is consistent with the methodology used inmore » the national cost-effectiveness analysis. Additional results and details on the methodology are presented in the following sections. The report provides analysis of two LCC scenarios: Scenario 1, representing publicly-owned buildings, considers initial costs, energy costs, maintenance costs, and replacement costs—without borrowing or taxes. Scenario 2, representing privately-owned buildings, adds borrowing costs and tax impacts.« less

Cost Effectiveness of ASHRAE Standard 90.1-2013 for the State of Washington

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hart, Philip R.; Athalye, Rahul A.; Xie, YuLong

2015-12-01

Moving to the ASHRAE Standard 90.1-2013 (ASHRAE 2013) edition from Standard 90.1-2010 (ASHRAE 2010) is cost-effective for the State of Washington. The table below shows the state-wide economic impact of upgrading to Standard 90.1-2013 in terms of the annual energy cost savings in dollars per square foot, additional construction cost per square foot required by the upgrade, and life-cycle cost (LCC) per square foot. These results are weighted averages for all building types in all climate zones in the state, based on weightings shown in Table 4. The methodology used for this analysis is consistent with the methodology used inmore » the national cost-effectiveness analysis. Additional results and details on the methodology are presented in the following sections. The report provides analysis of two LCC scenarios: Scenario 1, representing publicly-owned buildings, considers initial costs, energy costs, maintenance costs, and replacement costs—without borrowing or taxes. Scenario 2, representing privately-owned buildings, adds borrowing costs and tax impacts.« less

Cost Effectiveness of ASHRAE Standard 90.1-2013 for the State of Montana

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hart, Philip R.; Athalye, Rahul A.; Xie, YuLong

2015-12-01

Moving to the ASHRAE Standard 90.1-2013 (ASHRAE 2013) edition from Standard 90.1-2010 (ASHRAE 2010) is cost-effective for the State of Montana. The table below shows the state-wide economic impact of upgrading to Standard 90.1-2013 in terms of the annual energy cost savings in dollars per square foot, additional construction cost per square foot required by the upgrade, and life-cycle cost (LCC) per square foot. These results are weighted averages for all building types in all climate zones in the state, based on weightings shown in Table 4. The methodology used for this analysis is consistent with the methodology used inmore » the national cost-effectiveness analysis. Additional results and details on the methodology are presented in the following sections. The report provides analysis of two LCC scenarios: Scenario 1, representing publicly-owned buildings, considers initial costs, energy costs, maintenance costs, and replacement costs—without borrowing or taxes. Scenario 2, representing privately-owned buildings, adds borrowing costs and tax impacts.« less

Molecular cloning of the cDNA encoding laccase from Trametes versicolor and heterologous expression in Pichia methanolica.

PubMed

Guo, Mei; Lu, Fuping; Pu, Jun; Bai, Dongqing; Du, Lianxiang

2005-11-01

A cDNA encoding for laccase was isolated from the ligninolytic fungus Trametes versicolor by RNA-PCR. The cDNA corresponds to the gene Lcc1, which encodes a laccase isoenzyme of 498 amino acid residues preceded by a 22-residue signal peptide. The Lcc1 cDNA was cloned into the vectors pMETA and pMETalphaA and expressed in Pichia methanolica. The laccase activity obtained with the Saccharomyces cerevisiae alpha-factor signal peptide was found to be twofold higher than that obtained with the native secretion signal peptide. The extracellular laccase activity in recombinants with the alpha-factor signal peptide was 9.79 U ml(-1). The presence of 0.2 mM copper was necessary for optimal activity of laccase. The expression level was favoured by lower cultivation temperature. The identity of the recombinant protein was further confirmed by immunodetection using Western blot analysis. As expected, the molecular mass of the mature laccase was 64.0 kDa, similar to that of the native form.

Cost Effectiveness of ASHRAE Standard 90.1-2013 for the District of Columbia

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hart, Philip R.; Athalye, Rahul A.; Xie, YuLong

2015-12-01

Moving to the ASHRAE Standard 90.1-2013 (ASHRAE 2013) edition from Standard 90.1-2010 (ASHRAE 2010) is cost-effective for the District of Columbia. The table below shows the state-wide economic impact of upgrading to Standard 90.1-2013 in terms of the annual energy cost savings in dollars per square foot, additional construction cost per square foot required by the upgrade, and life-cycle cost (LCC) per square foot. These results are weighted averages for all building types in all climate zones in the state, based on weightings shown in Table 4. The methodology used for this analysis is consistent with the methodology used inmore » the national cost-effectiveness analysis. Additional results and details on the methodology are presented in the following sections. The report provides analysis of two LCC scenarios: Scenario 1, representing publicly-owned buildings, considers initial costs, energy costs, maintenance costs, and replacement costs—without borrowing or taxes. Scenario 2, representing privately-owned buildings, adds borrowing costs and tax impacts.« less

Cost Effectiveness of ASHRAE Standard 90.1-2013 for the State of Massachusetts

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hart, Philip R.; Athalye, Rahul A.; Xie, YuLong

2015-12-01

Moving to the ASHRAE Standard 90.1-2013 (ASHRAE 2013) edition from Standard 90.1-2010 (ASHRAE 2010) is cost-effective for the State of Massachusetts. The table below shows the state-wide economic impact of upgrading to Standard 90.1-2013 in terms of the annual energy cost savings in dollars per square foot, additional construction cost per square foot required by the upgrade, and life-cycle cost (LCC) per square foot. These results are weighted averages for all building types in all climate zones in the state, based on weightings shown in Table 4. The methodology used for this analysis is consistent with the methodology used inmore » the national cost-effectiveness analysis. Additional results and details on the methodology are presented in the following sections. The report provides analysis of two LCC scenarios: Scenario 1, representing publicly-owned buildings, considers initial costs, energy costs, maintenance costs, and replacement costs—without borrowing or taxes. Scenario 2, representing privately-owned buildings, adds borrowing costs and tax impacts.« less

Characterization of the IMF By-dependent field-aligned currents in the cleft region based on DE 2 observations

NASA Technical Reports Server (NTRS)

Taguchi, S.; Sugiura, M.; Winningham, J. D.; Slavin, J. A.

1993-01-01

The magnetic field and plasma data from 47 passes of DE-2 are used to study the IMF By-dependent distribution of field-aligned currents in the cleft region. It is proposed that the low-latitude cleft current (LCC) region is not an extension of the region 1 or region 2 current system and that a pair of LCCs and high-latitude cleft currents (HCCs) constitutes the cleft field-aligned current regime. The proposed pair of cleft field-aligned currents is explained with a qualitative model in which this pair of currents is generated on open field lines that have just been reconnected on the dayside magnetopause. The electric fields are transmitted along the field lines to the ionosphere, creating a poleward electric field and a pair of field-aligned currents when By is positive; the pair of field-aligned currents consists of a downward current at lower latitudes and an upward current at higher latitudes. In the By negative case, the model explains the reversal of the field-aligned current direction in the LCC and HCC regions.

Cost Effectiveness of ASHRAE Standard 90.1-2013 for the State of Oregon

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hart, Philip R.; Athalye, Rahul A.; Xie, YuLong

2015-12-01

Moving to the ASHRAE Standard 90.1-2013 (ASHRAE 2013) edition from Standard 90.1-2010 (ASHRAE 2010) is cost-effective for the State of Oregon. The table below shows the state-wide economic impact of upgrading to Standard 90.1-2013 in terms of the annual energy cost savings in dollars per square foot, additional construction cost per square foot required by the upgrade, and life-cycle cost (LCC) per square foot. These results are weighted averages for all building types in all climate zones in the state, based on weightings shown in Table 4. The methodology used for this analysis is consistent with the methodology used inmore » the national cost-effectiveness analysis. Additional results and details on the methodology are presented in the following sections. The report provides analysis of two LCC scenarios: Scenario 1, representing publicly-owned buildings, considers initial costs, energy costs, maintenance costs, and replacement costs—without borrowing or taxes. Scenario 2, representing privately-owned buildings, adds borrowing costs and tax impacts.« less

Cost Effectiveness of ASHRAE Standard 90.1-2013 for the State of Wisconsin

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hart, Philip R.; Athalye, Rahul A.; Xie, YuLong

2015-12-01

Moving to the ASHRAE Standard 90.1-2013 (ASHRAE 2013) edition from Standard 90.1-2010 (ASHRAE 2010) is cost-effective for the State of Wisconsin. The table below shows the state-wide economic impact of upgrading to Standard 90.1-2013 in terms of the annual energy cost savings in dollars per square foot, additional construction cost per square foot required by the upgrade, and life-cycle cost (LCC) per square foot. These results are weighted averages for all building types in all climate zones in the state, based on weightings shown in Table 4. The methodology used for this analysis is consistent with the methodology used inmore » the national cost-effectiveness analysis. Additional results and details on the methodology are presented in the following sections. The report provides analysis of two LCC scenarios: Scenario 1, representing publicly-owned buildings, considers initial costs, energy costs, maintenance costs, and replacement costs—without borrowing or taxes. Scenario 2, representing privately-owned buildings, adds borrowing costs and tax impacts.« less

Cost Effectiveness of ASHRAE Standard 90.1-2013 for the State of Ohio

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hart, Philip R.; Athalye, Rahul A.; Xie, YuLong

2015-12-01

Moving to the ASHRAE Standard 90.1-2013 (ASHRAE 2013) edition from Standard 90.1-2010 (ASHRAE 2010) is cost-effective for the State of Ohio. The table below shows the state-wide economic impact of upgrading to Standard 90.1-2013 in terms of the annual energy cost savings in dollars per square foot, additional construction cost per square foot required by the upgrade, and life-cycle cost (LCC) per square foot. These results are weighted averages for all building types in all climate zones in the state, based on weightings shown in Table 4. The methodology used for this analysis is consistent with the methodology used inmore » the national cost-effectiveness analysis. Additional results and details on the methodology are presented in the following sections. The report provides analysis of two LCC scenarios: Scenario 1, representing publicly-owned buildings, considers initial costs, energy costs, maintenance costs, and replacement costs—without borrowing or taxes. Scenario 2, representing privately-owned buildings, adds borrowing costs and tax impacts.« less

Cost Effectiveness of ASHRAE Standard 90.1-2013 for the State of South Carolina

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hart, Philip R.; Athalye, Rahul A.; Xie, YuLong

2015-12-01

Moving to the ASHRAE Standard 90.1-2013 (ASHRAE 2013) edition from Standard 90.1-2010 (ASHRAE 2010) is cost-effective for the State of South Carolina. The table below shows the state-wide economic impact of upgrading to Standard 90.1-2013 in terms of the annual energy cost savings in dollars per square foot, additional construction cost per square foot required by the upgrade, and life-cycle cost (LCC) per square foot. These results are weighted averages for all building types in all climate zones in the state, based on weightings shown in Table 4. The methodology used for this analysis is consistent with the methodology usedmore » in the national cost-effectiveness analysis. Additional results and details on the methodology are presented in the following sections. The report provides analysis of two LCC scenarios: Scenario 1, representing publicly-owned buildings, considers initial costs, energy costs, maintenance costs, and replacement costs—without borrowing or taxes. Scenario 2, representing privately-owned buildings, adds borrowing costs and tax impacts.« less

Cost Effectiveness of ASHRAE Standard 90.1-2013 for the State of North Carolina

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hart, Philip R.; Athalye, Rahul A.; Xie, YuLong

2015-12-01

Moving to the ASHRAE Standard 90.1-2013 (ASHRAE 2013) edition from Standard 90.1-2010 (ASHRAE 2010) is cost-effective for the State of North Carolina. The table below shows the state-wide economic impact of upgrading to Standard 90.1-2013 in terms of the annual energy cost savings in dollars per square foot, additional construction cost per square foot required by the upgrade, and life-cycle cost (LCC) per square foot. These results are weighted averages for all building types in all climate zones in the state, based on weightings shown in Table 4. The methodology used for this analysis is consistent with the methodology usedmore » in the national cost-effectiveness analysis. Additional results and details on the methodology are presented in the following sections. The report provides analysis of two LCC scenarios: Scenario 1, representing publicly-owned buildings, considers initial costs, energy costs, maintenance costs, and replacement costs—without borrowing or taxes. Scenario 2, representing privately-owned buildings, adds borrowing costs and tax impacts.« less

Cost Effectiveness of ASHRAE Standard 90.1-2013 for the State of Iowa

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hart, Philip R.; Athalye, Rahul A.; Xie, YuLong

2015-12-01

Moving to the ASHRAE Standard 90.1-2013 (ASHRAE 2013) edition from Standard 90.1-2010 (ASHRAE 2010) is cost-effective for the State of Iowa. The table below shows the state-wide economic impact of upgrading to Standard 90.1-2013 in terms of the annual energy cost savings in dollars per square foot, additional construction cost per square foot required by the upgrade, and life-cycle cost (LCC) per square foot. These results are weighted averages for all building types in all climate zones in the state, based on weightings shown in Table 4. The methodology used for this analysis is consistent with the methodology used inmore » the national cost-effectiveness analysis. Additional results and details on the methodology are presented in the following sections. The report provides analysis of two LCC scenarios: Scenario 1, representing publicly-owned buildings, considers initial costs, energy costs, maintenance costs, and replacement costs—without borrowing or taxes. Scenario 2, representing privately-owned buildings, adds borrowing costs and tax impacts.« less

Cradle-to-grave analysis on batik cabut product to reduce environmental impact

NASA Astrophysics Data System (ADS)

Djunaidi, Much.; Nursanti, Ida; Suryadarmawan, Viditwo Ashari

2017-06-01

Nowadays, the development of batik industry is growing rapidly. Efforts to protect the environment are increasing fast as well. This makes both of them go in line. In one of batik producers residing in Kampung Batik Laweyan, the process of making batik needs raw and supporting materials, both of which contain chemicals potentially dangerous to the environment. Assessment methods, such as life cycle assessment (LCA) and life cycle cost (LCC), to determine the value of the environmental impact are, therefore, needed. LCA and LCC are used to assess the level of product eco-efficiency. The eco-efficiency combines concepts of economic efficiency and efficiency of environmental resources. Based on assessment of 3,120 pieces of batik cabut product, it is found that scores of life cycle assessment (SLCA) and of life cycle cost (SLCC) are 4,049.15 points and IDR 146,437,138.29, respectively. Improvements proposed in this research are by replacing synthetic dyes with natural ones and by substituting wood with LPG. The proposal can reduce the environmental impact as much as 6.65%.

Analysis of the March 30, 2011 Hail Event at Shuttle Launch Pad 39A

NASA Technical Reports Server (NTRS)

Lane, John E.; Doesken, Nolan J.; Kasparis, Takis C.; Sharp, David W.

2012-01-01

The Kennedy Space Center (KSC) Hail Monitor System, a joint effort of the NASA KSC Physics Lab and the KSC Engineering Services Contract (ESC) Applied Technology Lab, was first deployed for operational testing in the fall of 2006. Volunteers from the Community Collaborative Rain, Hail, and Snow Network (CoCoRaHS) in conjunction with Colorado State University have been instrumental in validation testing using duplicate hail monitor systems at sites in the hail prone high plains of Colorado. The KSC Hail Monitor System (HMS), consisting of three stations positioned approximately 500 ft from the launch pad and forming an approximate equilateral triangle, as shown in Figure 1, was first deployed to Pad 39B for support of STS-115. Two months later, the HMS was deployed to Pad 39A for support of STS-116. During support of STS-117 in late February 2007, an unusually intense (for Florida standards) hail event occurred in the immediate vicinity of the exposed space shuttle and launch pad. Hail data of this event was collected by the HMS and analyzed. Support of STS-118 revealed another important application of the hail monitor system. Ground Instrumentation personnel check the hail monitors daily when a vehicle is on the launch pad, with special attention after any storm suspected of containing hail. If no hail is recorded by the HMS, the vehicle and pad inspection team has no need to conduct a thorough inspection of the vehicle immediately following a storm. On the afternoon of July 13, 2007, hail on the ground was reported by observers at the Vertical Assembly Building (VAB) and Launch Control Center (LCC), about three miles west of Pad 39A, as well as at several other locations at KSC. The HMS showed no impact detections, indicating that the shuttle had not been damaged by any of the numerous hail events which occurred on that day.

Application and Comparison of Laboratory Parameters for Forecasting Severe Hand-Foot-Mouth Disease Using Logistic Regression, Discriminant Analysis and Decision Tree.

PubMed

Sui, Meili; Huang, Xueyong; Li, Yi; Ma, Xiaomei; Zhang, Chao; Li, Xingle; Chen, Zhijuan; Feng, Huifen; Ren, Jingchao; Wang, Fang; Xu, Bianli; Duan, Guangcai

2016-01-01

In recent years, the prevalence of hand-foot-mouth disease (HFMD) in China and some other countries has caused worldwide concern. Mild cases tend to recover within a week, while severe cases may progress rapidly and tend to have bad outcome. Since there is no vaccine for HFMD and anti-inflammatory treatment is not ideal. In this study, we aimed to establish a valid forecasting model for severe HFMD using common laboratory parameters. Retrospectively, 77 severe HFMD cases from Zhengzhou Children's hospital in the peaking period between years 2013 to 2015 were collected, with 77 mild HFMD cases in the same area. The study recorded common laboratory parameters to assist in establishment of the severe HFMD model. After screening the important variables using Mann-Whitney U test, the study also matched the logistic regression (LR), discriminant analysis (DA), and decision tree (DT) to make a comparison. Compared with that of the mild group, serum levels of WBC, PLT, PCT, MCV, MCH, LCR, SCR, LCC, GLO, CK-MB, K, S100, and B in the severe group were higher (p < 0.05), while MCR, EOR, BASOR, SCC, MCC, EO, BASO, NA, CL, T, Th, and Th/Ts were lower (p < 0.05). Five indicators including MCR, LCC, Th, CK-MB, and CL were screened out by LR and the same for DA, and five variables including EO, LCC, CL, GLO, and MCC screened out by DT. The area under the curve (AUC) of LR, DA, and DT was 0.805, 0.779 and 0.864, respectively. The findings were that common laboratory indexes were effectively used to distinguish the mild HFMD cases and severe HFMD cases by LR, DA, and DT, and DT had the best classification effect with an AUC of 0.864.

Influence Diagram Use With Respect to Technology Planning and Investment

NASA Technical Reports Server (NTRS)

Levack, Daniel J. H.; DeHoff, Bryan; Rhodes, Russel E.

2009-01-01

Influence diagrams are relatively simple, but powerful, tools for assessing the impact of choices or resource allocations on goals or requirements. They are very general and can be used on a wide range of problems. They can be used for any problem that has defined goals, a set of factors that influence the goals or the other factors, and a set of inputs. Influence diagrams show the relationship among a set of results and the attributes that influence them and the inputs that influence the attributes. If the results are goals or requirements of a program, then the influence diagram can be used to examine how the requirements are affected by changes to technology investment. This paper uses an example to show how to construct and interpret influence diagrams, how to assign weights to the inputs and attributes, how to assign weights to the transfer functions (influences), and how to calculate the resulting influences of the inputs on the results. A study is also presented as an example of how using influence diagrams can help in technology planning and investment. The Space Propulsion Synergy Team (SPST) used this technique to examine the impact of R&D spending on the Life Cycle Cost (LCC) of a space transportation system. The question addressed was the effect on the recurring and the non-recurring portions of LCC of the proportion of R&D resources spent to impact technology objectives versus the proportion spent to impact operational dependability objectives. The goals, attributes, and the inputs were established. All of the linkages (influences) were determined. The weighting of each of the attributes and each of the linkages was determined. Finally the inputs were varied and the impacts on the LCC determined and are presented. The paper discusses how each of these was accomplished both for credibility and as an example for future studies using influence diagrams for technology planning and investment planning.

Real-Time Shear Wave versus Transient Elastography for Predicting Fibrosis: Applicability, and Impact of Inflammation and Steatosis. A Non-Invasive Comparison.

PubMed

Poynard, Thierry; Pham, Tam; Perazzo, Hugo; Munteanu, Mona; Luckina, Elena; Elaribi, Djamel; Ngo, Yen; Bonyhay, Luminita; Seurat, Noemie; Legroux, Muriel; Ngo, An; Deckmyn, Olivier; Thabut, Dominique; Ratziu, Vlad; Lucidarme, Olivier

2016-01-01

Real-time shear wave elastography (2D-SWE) is a two-dimensional transient elastography and a competitor as a biomarker of liver fibrosis in comparison with the standard reference transient elastography by M probe (TE-M). The aims were to compare several criteria of applicability, and to assess inflammation and steatosis impact on elasticity values, two unmet needs. We took FibroTest as the fibrosis reference and ActiTest and SteatoTest as quantitative estimates of inflammation and steatosis. After standardization of estimates, analyses used curve fitting, quantitative Lin concordance coefficient [LCC], and multivariate logistic regression. A total of 2,251 consecutive patients were included. We validated the predetermined 0.2 kPa cut-off as a too low minimal elasticity value identifying not-reliable 2D-SWE results (LCC with FibroTest = 0.0281[-0.119;0.175]. Other criteria, elasticity CV, body mass index and depth of measures were not sufficiently discriminant. The applicability of 2D-SWE (95%CI) 89.6%(88.2-90.8), was significantly higher than that of TE, 85.6%(84.0-87.0; P<0.0001). In patients with non-advanced fibrosis (METAVIR F0F1F2), elasticity values estimated by 2D-SWE was less impacted by inflammation and steatosis than elasticity value estimated by TE-M: LCC (95%CI) 0.039 (0.021;0.058) vs 0.090 (0.068;0.112;P<0.01) and 0.105 (0.068;0.141) vs 0.192 (0.153;0.230; P<0.01) respectively. The three analyses methods gave similar results. Elasticity results including very low minimal signal in the region of interest should be considered not reliable. 2D-SWE had a higher applicability than TE, the reference elastography, with less impact of inflammation and steatosis especially in patients with non-advanced fibrosis, as presumed by blood tests. ClinicalTrials.gov NCT01927133.

Enhancement of doxorubicin cytotoxicity of human cancer cells by tyrosine kinase inhibition of insulin receptor and type I IGF receptor

PubMed Central

Zeng, Xianke; Zhang, Hua; Oh, Annabell; Zhang, Yan; Yee, Douglas

2015-01-01

The type I insulin-like growth factor receptor (IGF1R) contributes to cancer cell biology. Disruption of IGF1R signaling alone or in combination with cytotoxic agents has emerged as a new therapeutic strategy. Our laboratory has shown that sequential treatment with doxorubicin (DOX) and anti-IGF1R antibodies significantly enhanced the response to chemotherapy. In this study, we examined whether inhibition of the tyrosine kinase activity of this receptor family would also enhance chemotherapy response. Cis-3-[3-(4-methyl-piperazin-l-yl)-cyclobutyl]-1-(2-phenyl-quinolin-7-yl)-imidazo[1,5-a]pyrazin-8-ylamine (PQIP) inhibited IGF1R and insulin receptor (InsR) kinase activity and downstream activation of ERK1/2 and Akt in MCF-7 and LCC6 cancer cells. PQIP inhibited both monolayer growth and anchorage-independent growth in a dose-dependent manner. PQIP did not induce apoptosis, but rather, PQIP treatment was associated with an increase in autophagy. We examined whether sequential or combination therapy of PQIP with DOX could enhance growth inhibition. PQIP treatment together with DOX or DOX followed by PQIP significantly inhibited anchorage-independent growth in MCF-7 and LCC6 cells compared to single agent alone. In contrast, pre-treatment with PQIP followed by DOX did not enhance the cytotoxicity of DOX in vitro. Furthermore, OSI-906, a PQIP derivative, inhibited IGF-I signaling in LCC6 xenograft tumors in vivo. When given once a week, simultaneous administration of OSI-906 and DOX significantly enhanced the anti-tumor effect of DOX. In summary, these results suggest that timing and duration of the IGF1R/InsR tyrosine kinase inhibitors with chemotherapeutic agents should be evaluated in clinical trials. Long-term disruption of IGF1R/InsR may not be necessary when combined with cytotoxic chemotherapy. PMID:21850397

Estimating babassu palm density using automatic palm tree detection with very high spatial resolution satellite images.

PubMed

Dos Santos, Alessio Moreira; Mitja, Danielle; Delaître, Eric; Demagistri, Laurent; de Souza Miranda, Izildinha; Libourel, Thérèse; Petit, Michel

2017-05-15

High spatial resolution images as well as image processing and object detection algorithms are recent technologies that aid the study of biodiversity and commercial plantations of forest species. This paper seeks to contribute knowledge regarding the use of these technologies by studying randomly dispersed native palm tree. Here, we analyze the automatic detection of large circular crown (LCC) palm tree using a high spatial resolution panchromatic GeoEye image (0.50 m) taken on the area of a community of small agricultural farms in the Brazilian Amazon. We also propose auxiliary methods to estimate the density of the LCC palm tree Attalea speciosa (babassu) based on the detection results. We used the "Compt-palm" algorithm based on the detection of palm tree shadows in open areas via mathematical morphology techniques and the spatial information was validated using field methods (i.e. structural census and georeferencing). The algorithm recognized individuals in life stages 5 and 6, and the extraction percentage, branching factor and quality percentage factors were used to evaluate its performance. A principal components analysis showed that the structure of the studied species differs from other species. Approximately 96% of the babassu individuals in stage 6 were detected. These individuals had significantly smaller stipes than the undetected ones. In turn, 60% of the stage 5 babassu individuals were detected, showing significantly a different total height and a different number of leaves from the undetected ones. Our calculations regarding resource availability indicate that 6870 ha contained 25,015 adult babassu palm tree, with an annual potential productivity of 27.4 t of almond oil. The detection of LCC palm tree and the implementation of auxiliary field methods to estimate babassu density is an important first step to monitor this industry resource that is extremely important to the Brazilian economy and thousands of families over a large scale. Copyright © 2017 Elsevier Ltd. All rights reserved.

Lignin-carbohydrate complexes from sisal (Agave sisalana) and abaca (Musa textilis): chemical composition and structural modifications during the isolation process.

PubMed

Del Río, José C; Prinsen, Pepijn; Cadena, Edith M; Martínez, Ángel T; Gutiérrez, Ana; Rencoret, Jorge

2016-05-01

Two types of lignins occurred in different lignin-carbohydrate fractions, a lignin enriched in syringyl units, less condensed, preferentially associated with xylans, and a lignin with more guaiacyl units, more condensed, associated with glucans. Lignin-carbohydrate complexes (LCC) were isolated from the fibers of sisal (Agave sisalana) and abaca (Musa textilis) according to a plant biomass fractionation procedure recently developed and which was termed as "universally" applicable to any type of lignocellulosic material. Two LCC fractions, namely glucan-lignin (GL) and xylan-lignin (XL), were isolated and differed in the content and composition of carbohydrates and lignin. In both cases, GL fractions were enriched in glucans and comparatively depleted in lignin, whereas XL fractions were depleted in glucans, but enriched in xylans and lignin. Analysis by two-dimensional Nuclear Magnetic Resonance (2D-NMR) and Derivatization Followed by Reductive Cleavage (DFRC) indicated that the XL fractions were enriched in syringyl (S)-lignin units and β-O-4' alkyl-aryl ether linkages, whereas GL fractions have more guaiacyl (G)-lignin units and less β-O-4' alkyl-aryl ether linkages per lignin unit. The data suggest that the structural characteristics of the lignin polymers are not homogeneously distributed within the same plant and that two different lignin polymers with different composition and structure might be present. The analyses also suggested that acetates from hemicelluloses and the acyl groups (acetates and p-coumarates) attached to the γ-OH of the lignin side chains were extensively hydrolyzed and removed during the LCC fractionation process. Therefore, caution must be paid when using this fractionation approach for the structural characterization of plants with acylated hemicelluloses and lignins. Finally, several chemical linkages (phenylglycosides and benzyl ethers) could be observed to occur between lignin and xylans in these plants.

Identification and Characterization of Cells with Cancer Stem Cell Properties in Human Primary Lung Cancer Cell Lines

PubMed Central

Suo, Zhenhe; Munthe, Else; Solberg, Steinar; Ma, Liwei; Wang, Mengyu; Westerdaal, Nomdo Anton Christiaan; Kvalheim, Gunnar; Gaudernack, Gustav

2013-01-01

Lung cancer (LC) with its different subtypes is generally known as a therapy resistant cancer with the highest morbidity rate worldwide. Therapy resistance of a tumor is thought to be related to cancer stem cells (CSCs) within the tumors. There have been indications that the lung cancer is propagated and maintained by a small population of CSCs. To study this question we established a panel of 15 primary lung cancer cell lines (PLCCLs) from 20 fresh primary tumors using a robust serum-free culture system. We subsequently focused on identification of lung CSCs by studying these cell lines derived from 4 representative lung cancer subtypes such as small cell lung cancer (SCLC), large cell carcinoma (LCC), squamous cell carcinoma (SCC) and adenocarcinoma (AC). We identified a small population of cells strongly positive for CD44 (CD44high) and a main population which was either weakly positive or negative for CD44 (CD44low/−). Co-expression of CD90 further narrowed down the putative stem cell population in PLCCLs from SCLC and LCC as spheroid-forming cells were mainly found within the CD44highCD90+ sub-population. Moreover, these CD44highCD90+ cells revealed mesenchymal morphology, increased expression of mesenchymal markers N-Cadherin and Vimentin, increased mRNA levels of the embryonic stem cell related genes Nanog and Oct4 and increased resistance to irradiation compared to other sub-populations studied, suggesting the CD44highCD90+ population a good candidate for the lung CSCs. Both CD44highCD90+ and CD44highCD90− cells in the PLCCL derived from SCC formed spheroids, whereas the CD44low/− cells were lacking this potential. These results indicate that CD44highCD90+ sub-population may represent CSCs in SCLC and LCC, whereas in SCC lung cancer subtype, CSC potentials were found within the CD44high sub-population. PMID:23469181

Predictive value of background experiences and visual spatial ability testing on laparoscopic baseline performance among residents entering postgraduate surgical training.

PubMed

Louridas, Marisa; Quinn, Lauren E; Grantcharov, Teodor P

2016-03-01

Emerging evidence suggests that despite dedicated practice, not all surgical trainees have the ability to reach technical competency in minimally invasive techniques. While selecting residents that have the ability to reach technical competence is important, evidence to guide the incorporation of technical ability into selection processes is limited. Therefore, the purpose of the present study was to evaluate whether background experiences and 2D-3D visual spatial test results are predictive of baseline laparoscopic skill for the novice surgical trainee. First-year residents were studied. Demographic data and background surgical and non-surgical experiences were obtained using a questionnaire. Visual spatial ability was evaluated using the PicSOr, cube comparison (CC) and card rotation (CR) tests. Technical skill was assessed using the camera navigation (LCN) task and laparoscopic circle cut (LCC) task. Resident performance on these technical tasks was compared and correlated with the questionnaire and visual spatial findings. Previous experience in observing laparoscopic procedures was associated with significantly better LCN performance, and experience in navigating the laparoscopic camera was associated with significantly better LCC task results. Residents who scored higher on the CC test demonstrated a more accurate LCN path length score (r s(PL) = -0.36, p = 0.03) and angle path (r s(AP) = -0.426, p = 0.01) score when completing the LCN task. No other significant correlations were found between the visual spatial tests (PicSOr, CC or CR) and LCC performance. While identifying selection tests for incoming surgical trainees that predict technical skill performance is appealing, the surrogate markers evaluated correlate with specific metrics of surgical performance related to a single task but do not appear to reliably predict technical performance of different laparoscopic tasks. Predicting the acquisition of technical skills will require the development of a series of evidence-based tests that measure a number of innate abilities as well as their inherent interactions.

Relevance of long term time - Series of atmospheric parameters at a mountain observatory to models for climate change

NASA Astrophysics Data System (ADS)

Kancírová, M.; Kudela, K.; Erlykin, A. D.; Wolfendale, A. W.

2016-10-01

A detailed analysis has been made based on annual meteorological and cosmic ray data from the Lomnicky stit mountain observatory (LS, 2634 masl; 49.40°N, 20.22°E; vertical cut-off rigidity 3.85 GV), from the standpoint of looking for possible solar cycle (including cosmic ray) manifestations. A comparison of the mountain data with the Global average for the cloud cover in general shows no correlation but there is a possible small correlation for low clouds (LCC in the Global satellite data). However, whereas it cannot be claimed that cloud cover observed at Lomnicky stit (LSCC) can be used directly as a proxy for the Global LCC, its examination has value because it is an independent estimate of cloud cover and one that has a different altitude weighting to that adopted in the satellite-derived LCC. This statement is derived from satellite data (http://isccp.giss.nasa.gov/climanal7.html) which shows the time series for the period 1983-2010 for 9 cloud regimes. There is a significant correlation only between cosmic ray (CR) intensity (and sunspot number (SSN)) and the cloud cover of the types cirrus and stratus. This effect is mainly confined to the CR intensity minimum during the epoch around 1990, when the SSN was at its maximum. This fact, together with the present study of the correlation of LSCC with our measured CR intensity, shows that there is no firm evidence for a significant contribution of CR induced ionization to the local (or, indeed, Global) cloud cover. Pressure effects are the preferred cause of the cloud cover changes. A consequence is that there is no evidence favouring a contribution of CR to the Global Warming problem. Our analysis shows that the LS data are consistent with the Gas Laws for a stable mass of atmosphere.

Impacts of future deforestation and climate change on the hydrology of the Amazon Basin: a multi-model analysis with a new set of land-cover change scenarios

NASA Astrophysics Data System (ADS)

Guimberteau, Matthieu; Ciais, Philippe; Ducharne, Agnès; Boisier, Juan Pablo; Dutra Aguiar, Ana Paula; Biemans, Hester; De Deurwaerder, Hannes; Galbraith, David; Kruijt, Bart; Langerwisch, Fanny; Poveda, German; Rammig, Anja; Andres Rodriguez, Daniel; Tejada, Graciela; Thonicke, Kirsten; Von Randow, Celso; Von Randow, Rita C. S.; Zhang, Ke; Verbeeck, Hans

2017-03-01

Deforestation in Amazon is expected to decrease evapotranspiration (ET) and to increase soil moisture and river discharge under prevailing energy-limited conditions. The magnitude and sign of the response of ET to deforestation depend both on the magnitude and regional patterns of land-cover change (LCC), as well as on climate change and CO2 levels. On the one hand, elevated CO2 decreases leaf-scale transpiration, but this effect could be offset by increased foliar area density. Using three regional LCC scenarios specifically established for the Brazilian and Bolivian Amazon, we investigate the impacts of climate change and deforestation on the surface hydrology of the Amazon Basin for this century, taking 2009 as a reference. For each LCC scenario, three land surface models (LSMs), LPJmL-DGVM, INLAND-DGVM and ORCHIDEE, are forced by bias-corrected climate simulated by three general circulation models (GCMs) of the IPCC 4th Assessment Report (AR4). On average, over the Amazon Basin with no deforestation, the GCM results indicate a temperature increase of 3.3 °C by 2100 which drives up the evaporative demand, whereby precipitation increases by 8.5 %, with a large uncertainty across GCMs. In the case of no deforestation, we found that ET and runoff increase by 5.0 and 14 %, respectively. However, in south-east Amazonia, precipitation decreases by 10 % at the end of the dry season and the three LSMs produce a 6 % decrease of ET, which is less than precipitation, so that runoff decreases by 22 %. For instance, the minimum river discharge of the Rio Tapajós is reduced by 31 % in 2100. To study the additional effect of deforestation, we prescribed to the LSMs three contrasted LCC scenarios, with a forest decline going from 7 to 34 % over this century. All three scenarios partly offset the climate-induced increase of ET, and runoff increases over the entire Amazon. In the south-east, however, deforestation amplifies the decrease of ET at the end of dry season, leading to a large increase of runoff (up to +27 % in the extreme deforestation case), offsetting the negative effect of climate change, thus balancing the decrease of low flows in the Rio Tapajós. These projections are associated with large uncertainties, which we attribute separately to the differences in LSMs, GCMs and to the uncertain range of deforestation. At the subcatchment scale, the uncertainty range on ET changes is shown to first depend on GCMs, while the uncertainty of runoff projections is predominantly induced by LSM structural differences. By contrast, we found that the uncertainty in both ET and runoff changes attributable to uncertain future deforestation is low.

Using biological data to test climate change refugia

NASA Astrophysics Data System (ADS)

Morelli, T. L.; Maher, S. P.

2015-12-01

The concept of refugia has been discussed from theoretical and paleontological perspectives to address how populations persisted during periods of unfavorable climate. Recently, several studies have applied the idea to contemporary landscapes to identify locations that are buffered from climate change effects so as to favor greater persistence of valued resources relative to other areas. Refugia are now being discussed among natural resource agencies as a potential adaptation option in the face of anthropogenic climate change. Using downscaled climate data, we identified hypothetical refugial meadows in the Sierra Nevada and then tested them using survey and genetic data from Belding's ground squirrel (Urocitellus beldingi) populations. We predicted that refugial meadows would show higher genetic diversity, higher rates of occupancy and lower rates of extirpation over time. At each step of the research, we worked with managers to ensure the largest impact. Although no panacea, identifying climate change refugia could be an important strategy for prioritizing habitats for management intervention in order to conserve populations. This research was supported by the California LCC, the Northeast Climate Science Center, and NSF.

STS-55 pad abort: Engine 2011 oxidizer preburner augmented spark igniter check valve leak

NASA Technical Reports Server (NTRS)

1993-01-01

The STS-55 initial launch attempt of Columbia (OV102) was terminated on KSC launch pad A March 22, 1993 at 9:51 AM E.S.T. due to violation of an ME-3 (Engine 2011) Launch Commit Criteria (LCC) limit exceedance. The event description and timeline are summarized. Propellant loading was initiated on 22 March, 1993 at 1:15 AM EST. All SSME chill parameters and launch commit criteria (LCC) were nominal. At engine start plus 1.44 seconds, a Failure Identification (FID) was posted against Engine 2011 for exceeding the 50 psia Oxidizer Preburner (OPB) purge pressure redline. The engine was shut down at 1.50 seconds followed by Engines 2034 and 2030. All shut down sequences were nominal and the mission was safely aborted. The OPB purge pressure redline violation and the abort profile/overlay for all three engines are depicted. SSME Avionics hardware and software performed nominally during the incident. A review of vehicle data table (VDT) data and controller software logic revealed no failure indications other than the single FID 013-414, OPB purge pressure redline exceeded. Software logic was executed according to requirements and there was no anomalous controller software operation. Immediately following the abort, a Rocketdyne/NASA failure investigation team was assembled. The team successfully isolated the failure cause to the oxidizer preburner augmented spark igniter purge check valve not being fully closed due to contamination. The source of the contaminant was traced to a cut segment from a rubber O-ring which was used in a fine clean tool during valve production prior to 1992. The valve was apparently contaminated during its fabrication in 1985. The valve had performed acceptably on four previous flights of the engine, and SSME flight history shows 780 combined check valve flights without failure. The failure of an Engine 3 (SSME No. 2011) check valve to close was sensed by onboard engine instruments even though all other engine operations were normal. This resulted in an engine shutdown and safe sequential shutdown of all three engines prior to ignition of the solid boosters.

Personal and Formal Backgrounds as Factors Which Influence Linguistic and Cultural Competency in the Teaching of Mathematics

ERIC Educational Resources Information Center

Nguyen-Le, Khanh

2010-01-01

This dissertation addresses the need for effective teaching approaches in a society with increasing numbers of culturally and linguistically diverse (CLD) students. The focus of this study is on teachers' linguistic and cultural competency (LCC), which I define as teachers' critical thinking and sensitivity concerning issues of language and…

Identification of the Mechanisms Underlying Antiestrogen Resistance: Breast Cancer Research Partnership between FIU-UM Braman Family Breast Cancer Institute

DTIC Science & Technology

2012-06-01

D. Increase in TrxR by sulforaphane contributes to tam sensitivity in tam resistant LCC2 cells by increasing expression of p27. In: Proceedings of...9. Penney R., Felty, Q., Slingerland, J., and Roy, D. (2010) Modulation of thioredoxin reductase by sulforaphane may restore tamoxifen sensitivity

Lane Community College Library Policy Manual. First Edition.

ERIC Educational Resources Information Center

Macnaughtan, Don, Ed.

In support of the stated goals and objectives of Lane Community College (LCC) in Oregon, the college library is developing a unified program of library and media resources and services. The purpose of the program is to enhance instruction and learning in a manner consistent with the philosophy and curriculum of the college. The goals of the…

Digital Avionics Information System (DAIS): Impact of DAIS Concept on Life Cycle Cost--Supplement. Final Report.

ERIC Educational Resources Information Center

Goclowski, John C.; And Others

This supplement to a technical report providing the results of a preliminary investigation of the potential impact of the Digital Avionics Information System (DAIS) concept on system support personnel requirements and life cycle cost (LCC) includes: (1) additional details of the cost comparison of a hypothetical application of a conceptual…

Digital Avionics Information System (DAIS): Impact of DAIS Concept on Life Cycle Cost. Final Report.

ERIC Educational Resources Information Center

Goclowski, John C.; And Others

Designed to identify and quantify the potential impacts of the Digital Avionics Information System (DAIS) on weapon system personnel requirements and life cycle cost (LCC), this study postulated a typical close-air-support (CAS) mission avionics suite to serve as a basis for comparing present day and DAIS configuration specifications. The purpose…

Five Year Strategic Plan, 1993-1997, Lakeland Community College.

ERIC Educational Resources Information Center

Lakeland Community Coll., Mentor, OH.

In 1990, the newly formed Planning Advisory Committee at Lakewood Community College (LCC) in Mentor, Ohio, developed the college's first 5-year strategic plan with input from across the campus. This plan for 1993-97 updates the original 1990-95 plan and is designed to assist in the development of annual goals and objectives. Following an…

Bibliography of In-House and Contract Reports. Supplement 9.

DTIC Science & Technology

1981-02-01

11;il CasCil roads andl ril roads would significantlN rcduLcc tlic ~anIual labor IMnOls d III V, i 1111111 d il.1 . graphic data bascs. ile effort...GUIDE FOR CLIMA TE (Rpt. No. 5 in the ETL Series on Guides for Army Terrain Analysts) September 1980 Keywords: Climatic Analysis, Factor Mapping

Deflection Measurements on Propeller 5503 in Ahead and Crashback

DTIC Science & Technology

2016-10-01

the dots on the blade that were visible for the run . Not all points could be determined for each picture during each run . One issue discovered with...Channel (LCC) in February and April of 2009. The deflection of the blades was measured using defocused particle image velocimetry. Comparisons were made... Blade Deflection Measurement CalTech

Exploiting LCSH, LCC, and DDC To Retrieve Networked Resources: Issues and Challenges.

ERIC Educational Resources Information Center

Chan, Lois Mai

This paper examines how the nature of the World Wide Web and characteristics of networked resources affect subject access and analyzes the requirements of effective indexing and retrieval tools. The current and potential uses of existing tools and possible courses of future development are explored in the context of recent research. The first…

Statistical Analysis of the Association Between Subject Headings and Their Corresponding Class Notations in Science and Technology Monographs.

ERIC Educational Resources Information Center

Khosh-khui, Abolghasem

This study investigates the degree of relationship between scientific and technical subject headings and their corresponding class notations in the Dewey Decimal (DDC) and Library of Congress Classification (LCC) systems. The degree of association between a subject heading and its corresponding class of notation or notations is measured by…

Effects of weighting schemes on the identification of wildlife corridors generated with least-cost methods

Treesearch

Sean A. Parks; Kevin S. McKelvey; Michael K. Schwartz

2012-01-01

The importance of movement corridors for maintaining connectivity within metapopulations of wild animals is a cornerstone of conservation. One common approach for determining corridor locations is least-cost corridor (LCC) modeling, which uses algorithms within a geographic information system to search for routes with the lowest cumulative resistance between target...

A Study of Attrition among Students at LaGuardia Community College: Report of Findings.

ERIC Educational Resources Information Center

Kane, Parsons and Associates, Inc., New York, NY.

In 1981-82, a study was conducted to determine why students left LaGuardia Community College (LCC) before completing the requirements for an associate degree. Interviews were conducted with dropout students over the course of the study, and questionnaires were administered to entering and continuing students to provide a comparative framework. The…

Defense ADP Acquisition Study.

DTIC Science & Technology

1981-11-30

Logistics ALS - Advanced Logistics System AMP - ADPS Master Plan ANSI - American National Standards Institute APR - Agency Procurement Request ASD(C...Computers IRM - Information Resources Management ISO - International Standards Organization L LCC - Life Cycle Costs LCM - Life Cycle Management LE...man- agement in the process * Lack of a mission orientation . Lack of systems management and life cycle perspectives * Lack of effective leadership

Comparison of leaf color chart observations with digital photographs and spectral measurements for estimating maize leaf chlorophyll content

USDA-ARS?s Scientific Manuscript database

Crop nitrogen management is important world-wide, as much for small fields as it is for large operations. Developed as a non-destructive aid for estimating nitrogen content in rice crops, leaf color charts (LCC) are a numbered series of plastic panels that range from yellowgreen to dark green. By vi...

A Decision Support Model Using Life Cycle Cost (LCC) Analysis to Select Cost-Effective Alternatives for Hazardous Materials

DTIC Science & Technology

1993-09-01

AF89-071. Washington: GPO, 1989. 159 14. Diaz , SSgt Laura, 648 Med Sq/SGHL. Personal interview. Brooks AFB, San Antonio TX, 14 July 1993. 15. EA...Training Strateav of the Acauisition Management of Hazardous Materials Program. Draft Report. McLean VA, August 1991. 36. Moore, SSgt Alicia . AL/OEVP

Littoral Combat Ship (LCS) Civilian Aviation Alternative Support Study: Report of Findings and Recommendations

DTIC Science & Technology

2006-07-30

instructions for: amphibious class (LHA, LHD, LSD , LPD, LST), combatant class (CG 16, CG 47, DD 963, DDG 51, FFG 7, PHM 1, PG 1), command class (LCC 19...20376-7003Kevin Boissonneault – Kevin.Boissonneault@Navy.Mil NAVAIR 4.10.1.2 48110 Shaw Road Bldg 2187, Suite 1180 49. Patuxent River, MD 20670

After Three Years: A Status Report on the International High School at LaGuardia Community College.

ERIC Educational Resources Information Center

Lieberman, Janet E.; And Others

In 1985, the International High School (IHS) at LaGuardia Community College (LCC) in New York City was established to address the crisis in retention of high school aged immigrants with low English proficiency. IHS has had great success in improving student attendance, retention, academic achievement, and graduation. Because of its location on the…

An Evaluation of the ELNP e-Learning Quality Assurance Program: Perspectives of Gap Analysis and Innovation Diffusion

ERIC Educational Resources Information Center

Chen, Ming-Puu

2009-01-01

The purpose of this study was to examine the appropriateness of a nationwide quality assurance framework for e-learning from participants' perspectives. Two types of quality evaluation programs were examined in this study, including the e-Learning Service Certification program (eLSC) and the e-Learning Courseware Certification program (eLCC). Gap…

Environmental Scanning Project: A Dimensional Aspect of Needs Assessment in the Strategic Planning Process, Fall 1987.

ERIC Educational Resources Information Center

Lomax, Lynn A.

The environmental scanning project described in this report was undertaken at Lane Community College (LCC) in Eugene, Oregon, to enhance the college's strategic planning process by anticipating events that might differ from the economic, social, and political conditions of the present. First, an overview is provided of the purpose and intent of…

Land Warrior (LW)/Mounted Warrior (MW) DOTMLPF Assessment

DTIC Science & Technology

2007-06-01

the DOTMLPF impacts of equipping a Stryker battalion with MW and LW? 3) What is the estimated life cycle cost (LCC) of each LW BOIP alternative? 4...A R E D A T A Assessed costs included: - Support Equipment/Disposal. - Contractor Logistics Support. - Ensemble Hardware...DOTMLPF . 3 - Cost . LUT Navigation Experiment Lethality Experiment AMSAA TRAC-WSMR TRAC-WSMR ATEC LW Test Unit

Application of lag-k autocorrelation coefficient and the TGA signals approach to detecting and quantifying adulterations of extra virgin olive oil with inferior edible oils.

PubMed

Torrecilla, José S; García, Julián; García, Silvia; Rodríguez, Francisco

2011-03-04

The combination of lag-k autocorrelation coefficients (LCCs) and thermogravimetric analyzer (TGA) equipment is defined here as a tool to detect and quantify adulterations of extra virgin olive oil (EVOO) with refined olive (ROO), refined olive pomace (ROPO), sunflower (SO) or corn (CO) oils, when the adulterating agents concentration are less than 14%. The LCC is calculated from TGA scans of adulterated EVOO samples. Then, the standardized skewness of this coefficient has been applied to classify pure and adulterated samples of EVOO. In addition, this chaotic parameter has also been used to quantify the concentration of adulterant agents, by using successful linear correlation of LCCs and ROO, ROPO, SO or CO in 462 EVOO adulterated samples. In the case of detection, more than 82% of adulterated samples have been correctly classified. In the case of quantification of adulterant concentration, by an external validation process, the LCC/TGA approach estimates the adulterant agents concentration with a mean correlation coefficient (estimated versus real adulterant agent concentration) greater than 0.90 and a mean square error less than 4.9%. Copyright © 2011 Elsevier B.V. All rights reserved.

Techno-economic Analysis of Acid Gas Removal and Liquefaction for Pressurized LNG

NASA Astrophysics Data System (ADS)

Lee, S. H.; Seo, Y. K.; Chang, D. J.

2018-05-01

This study estimated the life cycle cost (LCC) of an acid gas removal and a liquefaction processes for Pressurized LNG (PLNG) production and compared the results with the cost of normal LNG production. PLNG is pressurized LNG that is liquefied at a higher pressure and temperature than normal LNG. Due to the high temperature, the energy for liquefaction is reduced. The allowable CO2 concentration in PLNG is increased up to 3 mol% when the product pressure 25 bar. An amine process with 35 wt% of diethanolamine (DEA) aqueous solution and a nitrogen expansion cycle were selected for the acid gas removal and the liquefaction processes, respectively. Two types of CO2 concentration in the feed gas were investigated to analyze their impacts on the acid gas removal unit. When the CO2 concentration was 5 mol%, the acid gas removal unit was required for both LNG and PLNG production. However, the acid gas removal unit was not necessary in PLNG when the concentration was 0.5 mol% and the pressure was higher than 15 bar. The results showed that the LCC of PLNG was reduced by almost 35% relative to that of LNG when the PLNG pressure was higher than 15 bar.

Environmental and economic assessment of a road safety product made with virgin and recycled HDPE: a comparative study.

PubMed

L Simões, Carla; Costa Pinto, Lígia M; Bernardo, C A

2013-01-15

The development of value-added products made from post-consumer plastic recyclates has become an important goal in the quest for a sustainable society. To attain such goal, tools with higher accuracy and wider scope are increasingly necessary. The present work describes the application of a Life Cycle Assessment (LCA)/Life Cycle Costing (LCC) integrated model, with inclusion of externalities (environmental and social costs), to Anti-Glare Lamellae (AGL) made with High Density Polyethylene (HDPE). It compares an AGL currently manufactured from virgin HDPE (current AGL) with an alternative one made with recycled HDPE (optional AGL). The results obtained show that neither the current nor the optional AGL depict the best environmental performance in all impact categories. Nevertheless, there is a clear overall environmental and economic advantage in replacing virgin HDPE with recycled HDPE. The present work also makes evident that the LCA/LCC integrated model allows the identification of economic and environmental win-win and trade-off situations related to the full life cycle of products. As such, its results can be used as valuable guidelines in product development. Copyright © 2012 Elsevier Ltd. All rights reserved.

Comparison of algae cultivation methods for bioenergy production using a combined life cycle assessment and life cycle costing approach.

PubMed

Resurreccion, Eleazer P; Colosi, Lisa M; White, Mark A; Clarens, Andres F

2012-12-01

Algae are an attractive energy source, but important questions still exist about the sustainability of this technology on a large scale. Two particularly important questions concern the method of cultivation and the type of algae to be used. This present study combines elements of life cycle analysis (LCA) and life cycle costing (LCC) to evaluate open pond (OP) systems and horizontal tubular photobioreactors (PBRs) for the cultivation of freshwater (FW) or brackish-to-saline water (BSW) algae. Based on the LCA, OPs have lower energy consumption and greenhouse gas emissions than PBRs; e.g., 32% less energy use for construction and operation. According to the LCC, all four systems are currently financially unattractive investments, though OPs are less so than PBRs. BSW species deliver better energy and GHG performance and higher profitability than FW species in both OPs and PBRs. Sensitivity analyses suggest that improvements in critical cultivation parameters (e.g., CO(2) utilization efficiency or algae lipid content), conversion parameters (e.g., anaerobic digestion efficiency), and market factors (e.g., costs of CO(2) and electricity, or sale prices for algae biodiesel) could alter these results. Copyright © 2012 Elsevier Ltd. All rights reserved.

Clinical initiatives linking Japanese and Swedish healthcare resources on cancer studies utilizing Biobank Repositories.

PubMed

Nishimura, Toshihide; Kawamura, Takeshi; Sugihara, Yutaka; Bando, Yasuhiko; Sakamoto, Shigeru; Nomura, Masaharu; Ikeda, Norihiko; Ohira, Tatsuo; Fujimoto, Junichiro; Tojo, Hiromasa; Hamakubo, Takao; Kodama, Tatsuhiko; Andersson, Roland; Fehniger, Thomas E; Kato, Harubumi; Marko-Varga, György

2014-12-01

The Tokyo Medical University Hospital in Japan and the Lund University hospital in Sweden have recently initiated a research program with the objective to impact on patient treatment by clinical disease stage characterization (phenotyping), utilizing proteomics sequencing platforms. By sharing clinical experiences, patient treatment principles, and biobank strategies, our respective clinical teams in Japan and Sweden will aid in the development of predictive and drug related protein biomarkers. Data from joint lung cancer studies are presented where protein expression from Neuro- Endocrine lung cancer (LCNEC) phenotype patients can be separated from Small cell- (SCLC) and Large Cell lung cancer (LCC) patients by deep sequencing and spectral counting analysis. LCNEC, a subtype of large cell carcinoma (LCC), is characterized by neuroendocrine differentiation that small cell lung carcinoma (SCLC) shares. Pre-therapeutic histological distinction between LCNEC and SCLC has so far been problematic, leading to adverse clinical outcome. An establishment of protein targets characteristic of LCNEC is quite helpful for decision of optimal therapeutic strategy by diagnosing individual patients. Proteoform annotation and clinical biobanking is part of the HUPO initiative (http://www.hupo.org) within chromosome 10 and chromosome 19 consortia.

HAT1 induces lung cancer cell apoptosis via up regulating Fas.

PubMed

Han, Na; Shi, Lei; Guo, Qiuyun; Sun, Wei; Yu, Yang; Yang, Li; Zhang, Xiaoxi; Zhang, Mengxian

2017-10-27

The dysfunction of apoptosis is one of the factors contributing to lung cancer (LC) growth. Histone acetyltransferase HAT1 can up regulate cell apoptosis. This study aims to investigate the mechanism by which HAT1 induces LC cell (LCC) apoptosis via up regulating the expression of Fas. In this study, the surgically removed human LC tissues were collected. LCCs were isolated from the LC tissues and analyzed for the expression of HAT1 and Fas by RT-qPCR and Western blotting. We observed that the expression of Fas was negatively correlated with PAR2 in LCCs. Activation of PAR2 suppressed the expression of Fas in normal lung epithelial cells. The expression of HAT1 was lower and positively correlated with Fas expression and negatively correlated with PAR2 expression in LCCs. Activation of PAR2 suppressed Fas expression in lung epithelial cells via inhibiting HAT1. Restoration of HAT1 expression restored Fas expression in LCCs and induced LCC apoptosis. In conclusion, less expression of HAT1 in LCCs was associated with the pathogenesis of LC. Up regulation of HAT1 expression in LCCs can induce LCCs apoptosis, which may be a potential novel therapy for the treatment of LC.

Stable calcium isotope composition of a pedogenic carbonate in forested ecosystem: the case of the needle fibre calcite (NFC).

NASA Astrophysics Data System (ADS)

Milliere, Laure; Verrecchia, Eric; Gussone, Nikolaus

2014-05-01

Calcium (Ca), carbon (C) and oxygen (O) are important elements in terrestrial environment, as their biogeochemical cycles are directly related to the storage of atmospheric carbon. Nevertheless, contrarily to C and O, Ca isotope composition has been only poorly studied in the terrestrial carbonates. Needle Fibre Calcite (NFC) is one of the most common pedogenic carbonates, unless its origin is still under debate. Recent studies explain its formation by precipitation inside fungal hyphae. Due to this possible biogenic origin, NFC can be considered as a potential bridge between the biochemistry (precipitation inside organic structure) and geochemistry (pedogenic carbonate related to soil conditions) of the Ca. Thus, the study of the Ca isotope composition of NFC seem to be of first interest in order to shed light on the behaviour of Ca in terrestrial environment, especially when precipitation of secondary carbonates is involved. The sampling site is situated in the Swiss Jura Mountains and has been chosen due to a previous complete study of the C and O isotope composition of NFC in relation to the ecosystem, which represent a good precondition for the understanding of the NFC Ca isotope signatures in this context. In this study, the implication of the fungi in the origin of NFC is investigated, by comparing the Ca isotope composition of NFC and a purely physicochemical calcite cement (LCC), both precipitated in the same environment. The δ44Ca signature of NFC and LCC crystals were used to determine possible differences of the precipitation rate during their formation. NFC and LCC have similar δ18O composition and are supposed to precipitate at the same temperature (Milliere et al., 2011a). Thus the study of Ca isotope composition of NFC seems to demonstrate that the elongated shape of the calcite needle can be explained by different precipitation processes than the rhombohedric calcite crystals precipitated in the same environment; and more precisely, the specific shape of NFC could be ascribed to a growth related to fungal organic molecules or potentially inside fungal hyphae. Three microscopic morphologies of NFC, previously defined (Milliere et al., 2011a), have been also examined in order to trace the evolution of the NFC inside the soil porosity. The Ca isotope composition of the simple needles, which are supposed to be the original form of NFC are the less fractionated compared to the soil solution, whereas the Ca isotope composition of the two other microscopic morphologies, namely the simple needle with nanofibres and the simple needle with overgrowths, are more fractionated, like the LCC, indicating potentially the influence of biogenic processes in the formation of the simple needles. Milliere L, Hasinger O, Bindschedler S, Cailleau G, Spangenberg JE, Verrecchia EP. 2011a. Stable carbon and oxygen isotopic signatures of pedogenic needle fibre calcite. Geoderma 161, 74-87.

A REVISED AGE FOR UPPER SCORPIUS AND THE STAR FORMATION HISTORY AMONG THE F-TYPE MEMBERS OF THE SCORPIUS-CENTAURUS OB ASSOCIATION

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pecaut, Mark J.; Mamajek, Eric E.; Bubar, Eric J.

2012-02-20

We present an analysis of the ages and star formation history of the F-type stars in the Upper Scorpius (US), Upper Centaurus-Lupus (UCL), and Lower Centaurus-Crux (LCC) subgroups of Scorpius-Centaurus (Sco-Cen), the nearest OB association. Our parent sample is the kinematically selected Hipparcos sample of de Zeeuw et al., restricted to the 138 F-type members. We have obtained classification-resolution optical spectra and have also determined the spectroscopic accretion disk fraction. With Hipparcos and 2MASS photometry, we estimate the reddening and extinction for each star and place the candidate members on a theoretical H-R diagram. For each subgroup we construct empiricalmore » isochrones and compare to published evolutionary tracks. We find that (1) our empirical isochrones are consistent with the previously published age-rank of the Sco-Cen subgroups; (2) subgroups LCC and UCL appear to reach the main-sequence turn-on at spectral types {approx}F4 and {approx}F2, respectively. An analysis of the A-type stars shows US reaching the main sequence at about spectral type {approx}A3. (3) The median ages for the pre-main-sequence members of UCL and LCC are 16 Myr and 17 Myr, respectively, in agreement with previous studies, however we find that (4) Upper Sco is much older than previously thought. The luminosities of the F-type stars in US are typically a factor of {approx}2.5 less luminous than predicted for a 5 Myr old population for four sets of evolutionary tracks. We re-examine the evolutionary state and isochronal ages for the B-, A-, and G-type Upper Sco members, as well as the evolved M supergiant Antares, and estimate a revised mean age for Upper Sco of 11 {+-} 1 {+-} 2 Myr (statistical, systematic). Using radial velocities and Hipparcos parallaxes we calculate a lower limit on the kinematic expansion age for Upper Sco of >10.5 Myr (99% confidence). However, the data are statistically consistent with no expansion. We reevaluate the inferred masses for the known substellar companions in Upper Sco using the revised age and find that the inferred masses are typically {approx}20%-70% higher than the original estimates which had assumed a much younger age; specifically, we estimate the mass of 1RXS J1609-2105b to be 14{sup +2}{sub -3} M{sub Jup}, suggesting that it is a brown dwarf rather than a planet. Finally, we find the fraction of F-type stars exhibiting H{alpha} emission and/or a K-band excess consistent with accretion to be 0/17 (<19%; 95% CL) in US at {approx}11 Myr, while UCL has 1/41 (2{sup +5}{sub -1}%; 68% CL) accretors and LCC has 1/50 (2{sup +4}{sub -1}%; 68% CL) accretors at {approx}16 Myr and {approx}17 Myr, respectively.« less

A Revised Age for Upper Scorpius and the Star Formation History among the F-type Members of the Scorpius-Centaurus OB Association

NASA Astrophysics Data System (ADS)

Pecaut, Mark J.; Mamajek, Eric E.; Bubar, Eric J.

2012-02-01

We present an analysis of the ages and star formation history of the F-type stars in the Upper Scorpius (US), Upper Centaurus-Lupus (UCL), and Lower Centaurus-Crux (LCC) subgroups of Scorpius-Centaurus (Sco-Cen), the nearest OB association. Our parent sample is the kinematically selected Hipparcos sample of de Zeeuw et al., restricted to the 138 F-type members. We have obtained classification-resolution optical spectra and have also determined the spectroscopic accretion disk fraction. With Hipparcos and 2MASS photometry, we estimate the reddening and extinction for each star and place the candidate members on a theoretical H-R diagram. For each subgroup we construct empirical isochrones and compare to published evolutionary tracks. We find that (1) our empirical isochrones are consistent with the previously published age-rank of the Sco-Cen subgroups; (2) subgroups LCC and UCL appear to reach the main-sequence turn-on at spectral types ~F4 and ~F2, respectively. An analysis of the A-type stars shows US reaching the main sequence at about spectral type ~A3. (3) The median ages for the pre-main-sequence members of UCL and LCC are 16 Myr and 17 Myr, respectively, in agreement with previous studies, however we find that (4) Upper Sco is much older than previously thought. The luminosities of the F-type stars in US are typically a factor of ~2.5 less luminous than predicted for a 5 Myr old population for four sets of evolutionary tracks. We re-examine the evolutionary state and isochronal ages for the B-, A-, and G-type Upper Sco members, as well as the evolved M supergiant Antares, and estimate a revised mean age for Upper Sco of 11 ± 1 ± 2 Myr (statistical, systematic). Using radial velocities and Hipparcos parallaxes we calculate a lower limit on the kinematic expansion age for Upper Sco of >10.5 Myr (99% confidence). However, the data are statistically consistent with no expansion. We reevaluate the inferred masses for the known substellar companions in Upper Sco using the revised age and find that the inferred masses are typically ~20%-70% higher than the original estimates which had assumed a much younger age; specifically, we estimate the mass of 1RXS J1609-2105b to be 14+2 -3 M Jup, suggesting that it is a brown dwarf rather than a planet. Finally, we find the fraction of F-type stars exhibiting Hα emission and/or a K-band excess consistent with accretion to be 0/17 (<19% 95% CL) in US at ~11 Myr, while UCL has 1/41 (2+5 -1% 68% CL) accretors and LCC has 1/50 (2+4 -1% 68% CL) accretors at ~16 Myr and ~17 Myr, respectively.

Inhibition of mTOR's Catalytic Site by PKI-587 Is a Promising Therapeutic Option for Gastroenteropancreatic Neuroendocrine Tumor Disease.

PubMed

Freitag, Helma; Christen, Friederike; Lewens, Florentine; Grass, Irina; Briest, Franziska; Iwaszkiewicz, Sara; Siegmund, Britta; Grabowski, Patricia

2017-01-01

The characteristic clinical heterogeneity and mostly slow-growing behavior of gastroenteropancreatic neuroendocrine neoplasms (GEP-NENs) cause problems in finding appropriate treatments. Thus, the current therapy options are not satisfactory. PKI-587 is a highly potent, novel dual inhibitor of PI3K and mTORC1/C2. We assessed the effects of PKI-587 in different GEP-NEN tumor models, including the poorly differentiated cell line LCC-18, and compared them with those of the established mTORC1 inhibitor everolimus. We treated BON, QGP-1, KRJ-I, and LCC-18 cell lines with increasing concentrations of the inhibitor PKI-587, and compared the results with those of everolimus and DMSO. We assessed the impact of the treatments on viability (WST-1 assay), on apoptotic processes (caspase 3/7 assay, JC-1), and on cell cycle regulation (flow cytometry). We determined alterations in signaling mediators by phosphor-specific Western blot analysis and conducted multiplexed gene expression analysis (nCounter® technology). In all cell lines, PKI-587 dose-dependently inhibited proliferation, whereas everolimus was less effective. Treatment with PKI-587 led to cell cycle arrest and induction of apoptosis and successfully suppressed activity of the direct mTORC1 target 4E-BP1, a crucial factor for tumor genesis only partially inhibited by everolimus. Gene expression analyses revealed relevant changes of RAS, MAPK, STAT, and PI3K pathway genes after treatment. Treatment-dependent and cell line-characteristic effects on AKT/Rb/E2F signaling regarding cell cycle control and apoptosis are extensively discussed in this paper. PI3K/mTOR dual targeting is a promising new therapeutic approach in neuroendocrine tumor disease that should be evaluated in further clinical trials. © 2016 The Author(s) Published by S. Karger AG, Basel.

The Impact of Environmental Light Intensity on Experimental Tumor Growth.

PubMed

Suckow, Mark A; Wolter, William R; Duffield, Giles E

2017-09-01

Cancer research requires for consistent models that minimize environmental variables. Within the typical laboratory animal housing facility, animals may be exposed to varying intensities of light as a result of cage type, cage position, light source, and other factors; however, studies evaluating the differential effect of light intensity during the light phase on tumor growth are lacking. The effect of cage face light intensity, as determined by cage rack position was evaluated with two tumor models using the C57Bl/6NHsd mouse and transplantable B16F10 melanoma cells or Lewis lung carcinoma (LLC) cells. Animals were housed in individually-ventilated cages placed at the top, middle, or bottom of the rack in a diagonal pattern so that the top cage was closest to the ceiling light source, and cage face light intensity was measured. Following a two-week acclimation period at the assigned cage position, animals were subcutaneously administered either 1.3×10 6 B16F10 melanoma cells or 2.5×10 5 Lewis lung carcinoma cells. Weights of excised tumors were measured following euthanasia 18 days (melanoma) or 21 days (LCC) after tumor cell administration. Cage face light intensity was significantly different depending on the location of the cage, with cages closest to the light source have the greatest intensity. Mean tumor weights were significantly less (p<0.001 for melanoma; p≤0.01 for LCC) in middle light intensity mice compared to high and low light intensity mice. The environmental light intensity to which experimental animals are exposed may vary markedly with cage location and can significantly influence experimental tumor growth, thus supporting the idea that light intensity should be controlled as an experimental variable for animals used in cancer research. Copyright© 2017, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

Linking statistically-and physically-based models for improved streamflow simulation in gaged and ungaged watersheds

Treesearch

Jacob LaFontaine; Lauren Hay; Stacey Archfield; William Farmer; Julie Kiang

2016-01-01

The U.S. Geological Survey (USGS) has developed a National Hydrologic Model (NHM) to support coordinated, comprehensive and consistent hydrologic model development, and facilitate the application of hydrologic simulations within the continental US. The portion of the NHM located within the Gulf Coastal Plains and Ozarks Landscape Conservation Cooperative (GCPO LCC) is...

Cost Effectiveness of Two versus Three Levels of Maintenance for Turbine Engines in the Air Force Inventory

DTIC Science & Technology

1990-09-01

increasing 2 complexity of Air Force weapon systems makes the estimation of LCC an extremely difficult task. To accomplish this, mathematical models are...maintenance is nearly horizontal. A reduction in this rate from 2.8 to 2.6 (approx. 7%j resulted in cost savings of around 0.1% for the three lvel concept. The

Collective Bargaining Agreement between Board of Trustees of Lower Columbia College District 13 and Lower Columbia Faculty Association, 1987-1990.

ERIC Educational Resources Information Center

Lower Columbia Coll., Longview, WA.

This contractual agreement between the Board of Trustees of Lower Columbia College (LCC) District 13 and the Lower Columbia College Faculty Association outlines the terms of employment for all academic employees of the district. The 13 articles in the agreement set forth provisions related to: (1) recognition of the association as exclusive…

New Generation Energy Efficient Refractory Application in Soaking Pits of Bhilai Steel Plant, Sail

NASA Astrophysics Data System (ADS)

Roy, Indranil; Chintaiah, Perumetla; Bhattacharya, Ajoy Kr.; Garai, Swapan Kr.; Ray Choudhury, Pankaj Kr.; Tiwari, Laksman

In Bhilai Steel Plant (BSP), soaking pits are used for heating ingots for successive rolling into blooms. Pits are operated at a temperature of around 1350°C. Mixed gas (Mixture of Blast Furnace gas & Coke Oven gas) of calorific value around 2040 kcal/Nm3 is used as fuel. The walls of soaking pits were lined with traditional 38% Al2O3 firebricks and top 500mm was cast with 70% Al2O3 low cement castable (LCC). This type of lining results in frequent damages due to hitting by ingots while being lifted from pit by overhead cranes thus affecting the availability of pit. Life of pits was 2 to 2.5 years in BSP with 3-4 cold repairs and 3-4 hot repairs. Energy loss through the wall is also quite high in this type of lining. To triumph over the limitations of the conventional lining, a lining design was developed for the walls which consist of special 70% Al2O3 LCC having high hot strength (HMOR) in combination with specially design flexible SS-304 anchors. Ceramic fiber blanket and insulation bricks were provided between castable and the metallic shell of the pit to minimize the heat loss. A heating schedule was developed and introduced based on available infrastructure at BSP for proper curing of modified LCC based lining. After introduction of modified lining, pit no. 14/2 is running for more than 2.5 years without any repair. To capitalize the success, two more pits i.e. 12/1 and 9/2 were converted to modified lining. These pits are also running satisfactorily for more than 1.5 years. The modification has resulted in higher availability with substantial increase in production. Shell temperature of the modified pits reduced to 90° - 140°C from 120° - 200°C of conventional pits. This shows reduction in heat loss through walls, resulting less fuel consumption and energy saving of about 18%.

Real-Time Shear Wave versus Transient Elastography for Predicting Fibrosis: Applicability, and Impact of Inflammation and Steatosis. A Non-Invasive Comparison

PubMed Central

Poynard, Thierry; Pham, Tam; Perazzo, Hugo; Munteanu, Mona; Luckina, Elena; Elaribi, Djamel; Ngo, Yen; Bonyhay, Luminita; Seurat, Noemie; Legroux, Muriel; Ngo, An; Deckmyn, Olivier; Thabut, Dominique; Ratziu, Vlad; Lucidarme, Olivier

2016-01-01

Background and Aims Real-time shear wave elastography (2D-SWE) is a two-dimensional transient elastography and a competitor as a biomarker of liver fibrosis in comparison with the standard reference transient elastography by M probe (TE-M). The aims were to compare several criteria of applicability, and to assess inflammation and steatosis impact on elasticity values, two unmet needs. Methods We took FibroTest as the fibrosis reference and ActiTest and SteatoTest as quantitative estimates of inflammation and steatosis. After standardization of estimates, analyses used curve fitting, quantitative Lin concordance coefficient [LCC], and multivariate logistic regression. Results A total of 2,251 consecutive patients were included. We validated the predetermined 0.2 kPa cut-off as a too low minimal elasticity value identifying not-reliable 2D-SWE results (LCC with FibroTest = 0.0281[-0.119;0.175]. Other criteria, elasticity CV, body mass index and depth of measures were not sufficiently discriminant. The applicability of 2D-SWE (95%CI) 89.6%(88.2–90.8), was significantly higher than that of TE, 85.6%(84.0–87.0; P<0.0001). In patients with non-advanced fibrosis (METAVIR F0F1F2), elasticity values estimated by 2D-SWE was less impacted by inflammation and steatosis than elasticity value estimated by TE-M: LCC (95%CI) 0.039 (0.021;0.058) vs 0.090 (0.068;0.112;P<0.01) and 0.105 (0.068;0.141) vs 0.192 (0.153;0.230; P<0.01) respectively. The three analyses methods gave similar results. Conclusions Elasticity results including very low minimal signal in the region of interest should be considered not reliable. 2D-SWE had a higher applicability than TE, the reference elastography, with less impact of inflammation and steatosis especially in patients with non-advanced fibrosis, as presumed by blood tests. Trial Registration ClinicalTrials.gov NCT01927133 PMID:27706177

IT Labs Proof-of-Concept Project: Technical Data Interoperability (TDI) Pathfinder Via Emerging Standards

NASA Technical Reports Server (NTRS)

Conroy, Mike; Gill, Paul; Ingalls, John; Bengtsson, Kjell

2014-01-01

No known system is in place to allow NASA technical data interoperability throughout the whole life cycle. Life Cycle Cost (LCC) will be higher on many developing programs if action isn't taken soon to join disparate systems efficiently. Disparate technical data also increases safety risks from poorly integrated elements. NASA requires interoperability and industry standards, but breaking legacy ways is a challenge.

Integrated Instrumentation and Sensor Systems Enabling Condition-Based Maintenance of Aerospace Equipment

DTIC Science & Technology

2012-01-01

systems . For some specific sensor requirements in the domains considered here, for example, assessing system behavior and component state in gas turbine ...Cost Objectives. In general , the implication of the suitability and life cycle cost [LCC] driven objectives for integrated instrumentation/sensor system ...section should be considered. In general , the systems engineering approach provided clear benefits in defining user significant IISS system requirements and

Correction to: Malachite Green and Crystal Violet Decolorization by Ganoderma lucidum and Pleurotus ostreatus Supernatant and by rGlLCC1 and rPOXA 1B Concentrates: Molecular Docking Analysis.

PubMed

Morales-Álvarez, Edwin D; Rivera-Hoyos, Claudia M; Poveda-Cuevas, Sergio A; Reyes-Guzmán, Edwin A; Pedroza-Rodríguez, Aura M; Reyes-Montaño, Edgar A; Poutou-Piñales, Raúl A

2017-12-01

The original version of this article unfortunately contained a mistake. The replacement image of Fig. 4 provided by the first corresponding author, Aura M. Pedroza-Rodríguez, is incorrect and that the originally submitted Fig. 4 should have been retained. The original article has been corrected.

A High Resolution Phase Shifting Interferometer.

NASA Astrophysics Data System (ADS)

Bayda, Michael; Bartscher, Christoph; Wilkinson, Allen

1997-03-01

Configuration, operation, and performance details of a high resolution phase shifting Twyman-Green interferometer are presented. The instrument was used for density relaxation experiments of very compressible liquid-vapor critical fluids.(A companion talk in the Nonequilibrium Phenomena session under Complex Fluids presents density equilibration work.) A sample assembly contained the cell, beam splitter, phase shifter, and mirrors inside a 6 cm diameter by 6 cm long aluminum cylinder. This sample assembly was contained inside a thermostat stable to 50 μK RMS deviation. A thin phase retarding Liquid Crystal Cell (LCC) was placed in the reference arm of the interferometer. The LCC provided four cumulative 90 degree phase shifts to produce four images used in computing each phase map. The Carré technique was used to calculate a phase value for each pixel from the four intensities of each pixel. Four images for one phase map could be acquired in less than two seconds. The spatial resolution was 25 μm. The phase resolution of the interferometer in a six second period was better than λ/400. The phase stability of the interferometer during 25 hours was better than λ/70. Factors affecting timing, resolution, and other phase shifting devices will be discussed. WWW Presentation

Exploring the role of green and blue infrastructure in reducing temperature in Iskandar Malaysia using remote sensing approach

NASA Astrophysics Data System (ADS)

Kanniah, K. D.; sheikhi, A.; Kang, C. S.

2014-02-01

Development of cities has led to various environmental problems as a consequence of non sustaibale town planning. One of the strategies to make cities a livable place and to achieve low levels of CO2 emissions (low carbon cities or LCC) is the integration of the blue and green infrastructure into the development and planning of new urban areas. Iskandar Malaysia (IM) located in the southern part of Malaysia is a special economic zone that has major urban centres. The planning of these urban centres will incorporate LCC strategies to achieve a sustainable development. The role of green (plants) and blue bodies (lakes and rivers) in moderating temperature in IM have been investigated in the current study. A remotely sensed satellite imagery was used to calculate the vegetation density and land surface temperature (LST). The effect of lakes in cooling the surrounding temperature was also investigated. Results show that increasing vegetation density by 1% can decrease the LST by 0.09°C. As for the water bodies we found as the distance increased from the lake side the temperature also increased about 1.7°C and the reduction in air humidity is 9% as the distance increased to 100 meter away from the lake.

Direct human DNA protection by Coriolus versicolor (Yunzhi) extract.

PubMed

Szeto, Yim Tong; Lau, Po Chun; Kalle, Wouter; Pak, Sok Cheon

2013-07-01

Scientific evidence has shown Coriolus versicolor (L. ex Fr.) Quel (also known as Yunzhi) has the role of immunomodulator in therapeutic effect. The aim of this in vitro study was to investigate the antioxidative effect of Yunzhi and to explore the mechanisms behind its DNA protection. Commercial Yunzhi extract was dissolved in water and diluted in five concentrations (10(1)-10(5) μg/L) with appropriate buffers. Lymphocytes harvested from three healthy subjects were incubated with Yunzhi extract for 30 min. Cells were then subjected to 5 min oxidant challenge by 45 μM hydrogen peroxide. The standard alkaline comet (SAC) assay and lysed cell comet (LCC) assay were performed in parallel. DNA damage of each treatment was scored under a fluorescence microscope and compared with the cells without Yunzhi pretreatment. U-shaped dose-response was seen in both versions of the comet assay. Yunzhi at 10(4) μg/L demonstrated a genoprotective effect against oxidative damage in the SAC assay (25% decrease in comet score). In the LCC assay, a trend of protection in lymphocytes was observed but it did not reach statistical significance. A direct antioxidant effect of Yunzhi against oxidant challenge on the DNA of lymphocytes was evidenced. The active component in Yunzhi was likely to be membrane permeable.

A global view of regulatory networks in lung cancer: An approach to understand homogeneity and heterogeneity.

PubMed

Lu, Jiapei; Wang, William; Xu, Menglin; Li, Yuping; Chen, Chengshui; Wang, Xiangdong

2017-02-01

A number of new biotechnologies are used to identify potential biomarkers for the early detection of lung cancer, enabling a personalized therapy to be developed in response. The combinatorial cross-regulation of hundreds of biological function-specific transcription factors (TFs) is defined as the understanding of regulatory networks of molecules within the cell. Here we integrated global databases with 537 patients with lung adenocarcinoma (ADC), 140 with lung squamous carcinoma (SCC), 9 with lung large-cell carcinoma (LCC), 56 with small-cell lung cancer (SCLC), and 590 without cancer with the understanding of TF functions. The present review aims at the homogeneity or heterogeneity of gene expression profiles among subtypes of lung cancer. About 5, 136, 52, or 16 up-regulated or 19, 24, 122, or 97down-regulated type-special TF genes were identified in ADC, SCC, LCC or SCLC, respectively. DNA-binding and transcription regulator activity associated genes play a dominant role in the differentiation of subtypes in lung cancer. Subtype-specific TF gene regulatory networks with elements should be an alternative for diagnostic and therapeutic targets for early identification of lung cancer and can provide insightful clues to etiology and pathogenesis. Copyright © 2016 Elsevier Ltd. All rights reserved.

Large Crater Clustering tool

NASA Astrophysics Data System (ADS)

Laura, Jason; Skinner, James A.; Hunter, Marc A.

2017-08-01

In this paper we present the Large Crater Clustering (LCC) tool set, an ArcGIS plugin that supports the quantitative approximation of a primary impact location from user-identified locations of possible secondary impact craters or the long-axes of clustered secondary craters. The identification of primary impact craters directly supports planetary geologic mapping and topical science studies where the chronostratigraphic age of some geologic units may be known, but more distant features have questionable geologic ages. Previous works (e.g., McEwen et al., 2005; Dundas and McEwen, 2007) have shown that the source of secondary impact craters can be estimated from secondary impact craters. This work adapts those methods into a statistically robust tool set. We describe the four individual tools within the LCC tool set to support: (1) processing individually digitized point observations (craters), (2) estimating the directional distribution of a clustered set of craters, back projecting the potential flight paths (crater clusters or linearly approximated catenae or lineaments), (3) intersecting projected paths, and (4) intersecting back-projected trajectories to approximate the local of potential source primary craters. We present two case studies using secondary impact features mapped in two regions of Mars. We demonstrate that the tool is able to quantitatively identify primary impacts and supports the improved qualitative interpretation of potential secondary crater flight trajectories.

Structural differences between the lignin-carbohydrate complexes present in wood and in chemical pulps.

PubMed

Lawoko, Martin; Henriksson, Gunnar; Gellerstedt, Göran

2005-01-01

Lignin-carbohydrate complexes (LCCs) were prepared in quantitative yield from spruce wood and from the corresponding kraft and oxygen-delignified pulps and were separated into different fractions on the basis of their carbohydrate composition. To obtain an understanding of the differences in lignin structure and reactivity within the various LCC fractions, thioacidolysis in combination with gas chromatography was used to quantify the content of beta-O-4 structures in the lignin. Periodate oxidation followed by determination of methanol was used to quantify the phenolic hydroxyl groups. Furthermore, size exclusion chromatography (SEC) of the thioacidolysis fractions was used to monitor any differences between the original molecular size distribution and that after the delignification processes. Characteristic differences between the various LCC fractions were observed, clearly indicating that two different forms of lignin are present in the wood fiber wall. These forms are linked to glucomannan and xylan, respectively. On pulping, the different LCCs have different reactivities. The xylan-linked lignin is to a large extent degraded, whereas the glucomannan-linked lignin undergoes a partial condensation to form more high molecular mass material. The latter seems to be rather unchanged during a subsequent oxygen-delignification stage. On the basis of these findings, a modified arrangement of the fiber wall polymers is suggested.

Life-Cycle-Cost Analysis of the Microwave Landing System Ground and Airborne Systems

DTIC Science & Technology

1981-10-01

constant 1980 dollars, with a production rate variability. Table S-3 presents the life-cycle costs by MLS configuration and total system implementation... PRODUCTION RATE VARIABILITY OVER A THREE-YEAR PFODUCTXION RUN (MILLIONS OF CONSTANT 1980 DOLLARS) Pruduction (Juantitl•e and Costs system Typ 75...Implementation strategies * Production schedules for MLS equipment The LCC was determined to be relatively insensitive to changes in MTBF. This was expected

ACARA user's manual

NASA Technical Reports Server (NTRS)

Stalnaker, Dale K.

1993-01-01

ACARA (Availability, Cost, and Resource Allocation) is a computer program which analyzes system availability, lifecycle cost (LCC), and resupply scheduling using Monte Carlo analysis to simulate component failure and replacement. This manual was written to: (1) explain how to prepare and enter input data for use in ACARA; (2) explain the user interface, menus, input screens, and input tables; (3) explain the algorithms used in the program; and (4) explain each table and chart in the output.

Identification of the Mechanisms Underlying Antiestrogen Resistance: Breast Cancer Research Partnership between FIU-UM Braman Family Breast Cancer Institute

DTIC Science & Technology

2008-06-01

inhibited in antiestrogen resistant breast cancer cell line LCC-2 when exposed to ebselen or by overexpression of the antioxidant enzyme MnSOD...treatment with the antioxidant ebselen as well as by the overexpression of the antioxidant enzyme MnSOD. We performed comparative studies with the...Tamoxifen and Fulvestrant when treated with the antioxidant ebselen . 6 Challenges and difficulties encountered: Training: Immediately upon

WREP: A wavelet-based technique for extracting the red edge position from reflectance spectra for estimating leaf and canopy chlorophyll contents of cereal crops

NASA Astrophysics Data System (ADS)

Li, Dong; Cheng, Tao; Zhou, Kai; Zheng, Hengbiao; Yao, Xia; Tian, Yongchao; Zhu, Yan; Cao, Weixing

2017-07-01

Red edge position (REP), defined as the wavelength of the inflexion point in the red edge region (680-760 nm) of the reflectance spectrum, has been widely used to estimate foliar chlorophyll content from reflectance spectra. A number of techniques have been developed for REP extraction in the past three decades, but most of them require data-specific parameterization and the consistence of their performance from leaf to canopy levels remains poorly understood. In this study, we propose a new technique (WREP) to extract REPs based on the application of continuous wavelet transform to reflectance spectra. The REP is determined by the zero-crossing wavelength in the red edge region of a wavelet transformed spectrum for a number of scales of wavelet decomposition. The new technique is simple to implement and requires no parameterization from the user as long as continuous wavelet transforms are applied to reflectance spectra. Its performance was evaluated for estimating leaf chlorophyll content (LCC) and canopy chlorophyll content (CCC) of cereal crops (i.e. rice and wheat) and compared with traditional techniques including linear interpolation, linear extrapolation, polynomial fitting and inverted Gaussian. Our results demonstrated that WREP obtained the best estimation accuracy for both LCC and CCC as compared to traditional techniques. High scales of wavelet decomposition were favorable for the estimation of CCC and low scales for the estimation of LCC. The difference in optimal scale reveals the underlying mechanism of signature transfer from leaf to canopy levels. In addition, crop-specific models were required for the estimation of CCC over the full range. However, a common model could be built with the REPs extracted with Scale 5 of the WREP technique for wheat and rice crops when CCC was less than 2 g/m2 (R2 = 0.73, RMSE = 0.26 g/m2). This insensitivity of WREP to crop type indicates the potential for aerial mapping of chlorophyll content between growth seasons of cereal crops. The new REP extraction technique provides us a new insight for understanding the spectral changes in the red edge region in response to chlorophyll variation from leaf to canopy levels.

Ranking of Air Force Heating Plants Relative to the Economic Benefit of Coal Utilization

DTIC Science & Technology

1989-11-01

HTlW Output Capacity ..................... 27 5.2.2 Combustion Technologies ......................... 31 5.3 COMPUTER MODEL FOR LCC ANALISIS ...and field-erected units have been examined. The packaged units are factory -built, shell (fire-tube) boilers that are small enotgh to be shipped by...40 HBtMu/h with a thermal energy capacity factory of about 65% if used as a baseload heating plant. A water- tube boiler with a steam rating of 1200

Exact Recovery of Chaotic Systems from Highly Corrupted Data

DTIC Science & Technology

2016-08-01

dimension to reconstruct a state space which preserves the topological properties of the original system. In [CM87, RS92], the authors use the singular...in high dimensional nonlinear functional spaces [Spr94, SL00, LCC04]. In this work, we bring together connections between compressed sensing, splitting... compact , connected attractor Λ and the flow admits a unique so-called “physical" measure µ with supp(µ) = Λ. An invariant probability measure µ for a flow

Consistent Yokoya-Chen Approximation to Beamstrahlung(LCC-0010)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Peskin, M

2004-04-22

I reconsider the Yokoya-Chen approximate evolution equation for beamstrahlung and modify it slightly to generate simple, consistent analytical approximations for the electron and photon energy spectra. I compare these approximations to previous ones, and to simulation data.I reconsider the Yokoya-Chen approximate evolution equation for beamstrahlung and modify it slightly to generate simple, consistent analytical approximations for the electron and photon energy spectra. I compare these approximations to previous ones, and to simulation data.

JFMCC: Theater C2 in Need of SOLE

DTIC Science & Technology

2003-02-03

four command ships in service presently in the Navy. “Of the four command ships in service today, two—the Mount Whitney (LCC-20) and the Blue Ridge ...Dr. Henry H. Gaffney , Jr., The Top 100 Rules of the New American Way of War, (Washington, D.C.: Office of Force Transformation, Office of the...2001. Barnett, Thomas and Gaffney , Henry H. The Top 100 Rules of the New American Way of War. Washington, D.C.: Office of

Robustness testing in pharmaceutical freeze-drying: inter-relation of process conditions and product quality attributes studied for a vaccine formulation.

PubMed

Schneid, Stefan C; Stärtzel, Peter M; Lettner, Patrick; Gieseler, Henning

2011-01-01

The recent US Food and Drug Administration (FDA) legislation has introduced the evaluation of the Design Space of critical process parameters in manufacturing processes. In freeze-drying, a "formulation" is expected to be robust when minor deviations of the product temperature do not negatively affect the final product quality attributes. To evaluate "formulation" robustness by investigating the effect of elevated product temperature on product quality using a bacterial vaccine solution. The vaccine solution was characterized by freeze-dry microscopy to determine the critical formulation temperature. A conservative cycle was developed using the SMART™ mode of a Lyostar II freeze dryer. Product temperature was elevated to imitate intermediate and aggressive cycle conditions. The final product was analyzed using X-ray powder diffraction (XRPD), scanning electron microscopy (SEM), Karl Fischer, and modulated differential scanning calorimetry (MDSC), and the life cell count (LCC) during accelerated stability testing. The cakes processed at intermediate and aggressive conditions displayed larger pores with microcollapse of walls and stronger loss in LCC than the conservatively processed product, especially during stability testing. For all process conditions, a loss of the majority of cells was observed during storage. For freeze-drying of life bacterial vaccine solutions, the product temperature profile during primary drying appeared to be inter-related to product quality attributes.

Environmental and Economic Implications of Distributed Additive Manufacturing: The Case of Injection Mold Tooling: Environmental Implications of Additive Manufacturing

DOE Office of Scientific and Technical Information (OSTI.GOV)

Huang, Runze; Riddle, Matthew E.; Graziano, Diane

Additive manufacturing (AM) holds great potentials in enabling superior engineering functionality, streamlining supply chains, and reducing life cycle impacts compared to conventional manufacturing (CM). This study estimates the net changes in supply-chain lead time, life cycle primary energy consumption, greenhouse gas (GHG) emissions, and life cycle costs (LCC) associated with AM technologies for the case of injection molding, to shed light on the environmental and economic advantages of a shift from international or onshore CM to AM in the United States. A systems modeling framework is developed, with integrations of lead-time analysis, life cycle inventory analysis, LCC model, and scenariosmore » considering design differences, supply-chain options, productions, maintenance, and AM technological developments. AM yields a reduction potential of 3% to 5% primary energy, 4% to 7% GHG emissions, 12% to 60% lead time, and 15% to 35% cost over 1 million cycles of the injection molding production depending on the AM technology advancement in future. The economic advantages indicate the significant role of AM technology in raising global manufacturing competitiveness of local producers, while the relatively small environmental benefits highlight the necessity of considering trade-offs and balance techniques between environmental and economic performances when AM is adopted in the tooling industry. The results also help pinpoint the technological innovations in AM that could lead to broader benefits in future.« less

Bio-electro oxidation of indigo carmine by using microporous activated carbon fiber felt as anode and bioreactor support.

PubMed

Garcia, Luane Ferreira; Rodrigues Siqueira, Ana Claudia; Lobón, Germán Sanz; Marcuzzo, Jossano Saldanha; Pessela, Benevides Costa; Mendez, Eduardo; Garcia, Telma Alves; de Souza Gil, Eric

2017-11-01

The bioremediation and electro-oxidation (EO) processes are included among the most promising cleaning and decontamination mechanisms of water. The efficiency of bioremediation is dictated by the biological actuator for a specific substrate, its suitable immobilization and all involved biochemical concepts. The EO performance is defined by the anode efficiency to perform the complete mineralization of target compounds and is highlighted by the low or null use of reagent. Recently, the combination of both technologies has been proposed. Thus, the development of high efficient, low cost and eco-friendly anodes for sustainable EO, as well as, supporting devices for immobilization of biological systems applied in bioremediation is an open field of research. Therefore, the aim of this work was to promote the bio-electrochemical remediation of indigo carmine dye (widely common in textile industry), using new anode based on a microporous activated carbon fiber felt (ACFF) and ACFF with immobilized Laccase (Lcc) from Pycnoporus sanguineus. The results were discolorations of 62.7% with ACFF anode and 83.60% with ACFF-MANAE-Lcc anode, both for 60 min in tap water. This remediation rates show that this new anode has low cost and efficiency in the degradation of indigo dye and can be applied for other organic pollutant. Copyright © 2017 Elsevier Ltd. All rights reserved.

Environmental and Economic Implications of Distributed Additive Manufacturing: The Case of Injection Mold Tooling: Environmental Implications of Additive Manufacturing

DOE PAGES

Huang, Runze; Riddle, Matthew E.; Graziano, Diane; ...

2017-08-26

Additive manufacturing (AM) holds great potentials in enabling superior engineering functionality, streamlining supply chains, and reducing life cycle impacts compared to conventional manufacturing (CM). This study estimates the net changes in supply-chain lead time, life cycle primary energy consumption, greenhouse gas (GHG) emissions, and life cycle costs (LCC) associated with AM technologies for the case of injection molding, to shed light on the environmental and economic advantages of a shift from international or onshore CM to AM in the United States. A systems modeling framework is developed, with integrations of lead-time analysis, life cycle inventory analysis, LCC model, and scenariosmore » considering design differences, supply-chain options, productions, maintenance, and AM technological developments. AM yields a reduction potential of 3% to 5% primary energy, 4% to 7% GHG emissions, 12% to 60% lead time, and 15% to 35% cost over 1 million cycles of the injection molding production depending on the AM technology advancement in future. The economic advantages indicate the significant role of AM technology in raising global manufacturing competitiveness of local producers, while the relatively small environmental benefits highlight the necessity of considering trade-offs and balance techniques between environmental and economic performances when AM is adopted in the tooling industry. The results also help pinpoint the technological innovations in AM that could lead to broader benefits in future.« less

Economic and environmental evaluation model for selecting the optimum design of green roof systems in elementary schools.

PubMed

Kim, JiMin; Hong, TaeHoon; Koo, Choong-Wan

2012-08-07

Green-roof systems offer various benefits to man and nature, such as establishing ecological environments, improving landscape and air quality, and offering pleasant living environments. This study aimed to develop an optimal-scenario selection model that considers both the economic and the environmental effect in applying GRSs to educational facilities. The following process was carried out: (i) 15 GRSs scenarios were established by combining three soil and five plant types and (ii) the results of the life cycle CO(2) analyses with the GRSs scenarios were converted to an economic value using certified emission reductions (CERs) carbon credits. Life cycle cost (LCC) analyses were performed based on these results. The results showed that when considering only the currently realized economic value, the conventional roof system is superior to the GRSs. However, the LCC analysis that included the environmental value, revealed that compared to the conventional roof system, the following six GRSs scenarios are superior (cost reduction; reduction ratio; in descending order): scenarios 13 ($195,229; 11.0%), 3 ($188,178; 10.6%), 8 ($181,558; 10.3%), 12 ($130,464; 7.4%), 2 ($124,566; 7.0%), and 7 ($113,931; 6.4%). Although the effect is relatively small in terms of cost reduction, environmental value attributes cannot be ignored in terms of the reduction ratio.

Effect of Berry Extracts and Bioactive Compounds on Fulvestrant (ICI 182,780) Sensitive and Resistant Cell Lines.

PubMed

Woode, Denzel R; Aiyer, Harini S; Sie, Nicole; Zwart, Alan L; Li, Liya; Seeram, Navindra P; Clarke, Robert

2012-01-01

Fulvestrant (ICI 182,780; ICI) is approved for the treatment of advanced metastatic breast cancer that is unresponsive to other endocrine therapies. Berries are frequently consumed for their antioxidant, anti-inflammatory, and anticancer potential. In this study, we tested the efficacy of two berry extracts (Jamun-EJAE and red raspberry-RRE) and their bioactive compounds (Delphinidin-Del and Ellagic acid-EA) to inhibit cell proliferation with or without a sublethal dose of ICI in various breast cancer cell lines. ICI-sensitive (LCC1, ZR75-1, and BT474) and -resistant (LCC9, ZR75-1R) cells were subjected to treatment with berry extracts alone (0.1-100 μg/mL) or with a sub-lethal dose of ICI ( 1). EA, in doses tested, did not have any significant effects on any of the cell lines. Finally, we found that the extracts were more effective at lower, physiologically relevant concentrations than at higher experimental doses.

Effect of Berry Extracts and Bioactive Compounds on Fulvestrant (ICI 182,780) Sensitive and Resistant Cell Lines

PubMed Central

Woode, Denzel R.; Aiyer, Harini S.; Sie, Nicole; Zwart, Alan L.; Li, Liya; Seeram, Navindra P.; Clarke, Robert

2012-01-01

Fulvestrant (ICI 182,780; ICI) is approved for the treatment of advanced metastatic breast cancer that is unresponsive to other endocrine therapies. Berries are frequently consumed for their antioxidant, anti-inflammatory, and anticancer potential. In this study, we tested the efficacy of two berry extracts (Jamun-EJAE and red raspberry-RRE) and their bioactive compounds (Delphinidin-Del and Ellagic acid-EA) to inhibit cell proliferation with or without a sublethal dose of ICI in various breast cancer cell lines. ICI-sensitive (LCC1, ZR75-1, and BT474) and -resistant (LCC9, ZR75-1R) cells were subjected to treatment with berry extracts alone (0.1–100 μg/mL) or with a sub-lethal dose of ICI ( 1). EA, in doses tested, did not have any significant effects on any of the cell lines. Finally, we found that the extracts were more effective at lower, physiologically relevant concentrations than at higher experimental doses. PMID:23346406

Greening of the Earth and its drivers

DOE PAGES

Zhu, Zaichun; Piao, Shilong; Myneni, Ranga B.; ...

2016-04-25

Global environmental change is rapidly altering the dynamics of terrestrial vegetation, with consequences for the functioning of the Earth system and provision of ecosystem services 1, 2. Yet how global vegetation is responding to the changing environment is not well established. Here we use three long-term satellite leaf area index (LAI) records and ten global ecosystem models to investigate four key drivers of LAI trends during 1982 2009. We show a persistent and widespread increase of growing season integrated LAI (greening) over 25% to 50% of the global vegetated area, whereas less than 4% of the globe shows decreasing LAImore » (browning). Factorial simulations with multiple global ecosystem models suggest that CO 2 fertilization effects explain 70% of the observed greening trend, followed by nitrogen deposition (9%), climate change (8%) and land cover change (LCC) (4%). CO 2 fertilization effects explain most of the greening trends in the tropics, whereas climate change resulted in greening of the high latitudes and the Tibetan Plateau. LCC contributed most to the regional greening observed in southeast China and the eastern United States. In conclusion, the regional effects of unexplained factors suggest that the next generation of ecosystem models will need to explore the impacts of forest demography, differences in regional management intensities for cropland and pastures, and other emerging productivity constraints such as phosphorus availability.« less

New Low-Mass Wide Companions to Members of the Sco-Cen OB Association

NASA Astrophysics Data System (ADS)

Finn, Molly; Mamajek, Eric E.; Luhman, Kevin; Murphy, Simon

2017-01-01

We have conducted a survey for wide common proper motion companions to 512 members of the Upper Cen-Lup (UCL) and Lower Cen-Cru (LCC) subgroups of the Sco-Cen OB association using astrometry and photometry from the SPM4 catalog. Companion candidates were selected within 3 arcminutes (23 kau = 0.11 pc at d = 130 pc). We find that 12% of the Sco-Cen members have stellar companions in the separation range 3-20 kau. Optical spectra of 16 candidate companions were taken with the new COSMOS spectrograph on the Blanco 4-m telescope and the Wide Field Spectrograph on the Australian National University 2.3-m telescope. The spectroscopic survey yielded 14 M-type stars with Na I surface gravity index indicative of pre-MS status, of which half were Li-rich (along with two background giant interlopers). Seven stars in the range M1-M4.5 appear to be Li depleted, but otherwise seem to be pre-MS due to their chromospheric activity, low surface gravity via the Na I index, and their co- movement with other young stars in Sco-Cen. We suggest that these stars represent the Li depletion boundary for UCL and LCC. We acknowledge support from NSF award AST-1313029 and the REU Site in Physics and Astrophysics at the University of Rochester supported by NSF award PHY-1156339.

Greening of the Earth and its drivers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhu, Zaichun; Piao, Shilong; Myneni, Ranga B.

Global environmental change is rapidly altering the dynamics of terrestrial vegetation, with consequences for the functioning of the Earth system and provision of ecosystem services 1, 2. Yet how global vegetation is responding to the changing environment is not well established. Here we use three long-term satellite leaf area index (LAI) records and ten global ecosystem models to investigate four key drivers of LAI trends during 1982 2009. We show a persistent and widespread increase of growing season integrated LAI (greening) over 25% to 50% of the global vegetated area, whereas less than 4% of the globe shows decreasing LAImore » (browning). Factorial simulations with multiple global ecosystem models suggest that CO 2 fertilization effects explain 70% of the observed greening trend, followed by nitrogen deposition (9%), climate change (8%) and land cover change (LCC) (4%). CO 2 fertilization effects explain most of the greening trends in the tropics, whereas climate change resulted in greening of the high latitudes and the Tibetan Plateau. LCC contributed most to the regional greening observed in southeast China and the eastern United States. In conclusion, the regional effects of unexplained factors suggest that the next generation of ecosystem models will need to explore the impacts of forest demography, differences in regional management intensities for cropland and pastures, and other emerging productivity constraints such as phosphorus availability.« less

Benchmarking wastewater treatment plants under an eco-efficiency perspective.

PubMed

Lorenzo-Toja, Yago; Vázquez-Rowe, Ian; Amores, María José; Termes-Rifé, Montserrat; Marín-Navarro, Desirée; Moreira, María Teresa; Feijoo, Gumersindo

2016-10-01

The new ISO 14045 framework is expected to slowly start shifting the definition of eco-efficiency toward a life-cycle perspective, using Life Cycle Assessment (LCA) as the environmental impact assessment method together with a system value assessment method for the economic analysis. In the present study, a set of 22 wastewater treatment plants (WWTPs) in Spain were analyzed on the basis of eco-efficiency criteria, using LCA and Life Cycle Costing (LCC) as a system value assessment method. The study is intended to be useful to decision-makers in the wastewater treatment sector, since the combined method provides an alternative scheme for analyzing the relationship between environmental impacts and costs. Two midpoint impact categories, global warming and eutrophication potential, as well as an endpoint single score indicator were used for the environmental assessment, while LCC was used for value assessment. Results demonstrated that substantial differences can be observed between different WWTPs depending on a wide range of factors such as plant configuration, plant size or even legal discharge limits. Based on these results the benchmarking of wastewater treatment facilities was performed by creating a specific classification and certification scheme. The proposed eco-label for the WWTPs rating is based on the integration of the three environmental indicators and an economic indicator calculated within the study under the eco-efficiency new framework. Copyright © 2016 Elsevier B.V. All rights reserved.

Amplified in Breast Cancer Regulates Transcription and Translation in Breast Cancer Cells.

PubMed

Ochnik, Aleksandra M; Peterson, Mark S; Avdulov, Svetlana V; Oh, Annabell S; Bitterman, Peter B; Yee, Douglas

2016-02-01

Control of mRNA translation is fundamentally altered in cancer. Insulin-like growth factor-I (IGF-I) signaling regulates key translation mediators to modulate protein synthesis (e.g. eIF4E, 4E-BP1, mTOR, and S6K1). Importantly the Amplified in Breast Cancer (AIB1) oncogene regulates transcription and is also a downstream mediator of IGF-I signaling. To determine if AIB1 also affects mRNA translation, we conducted gain and loss of AIB1 function experiments in estrogen receptor alpha (ERα)(+) (MCF-7L) and ERα(-) (MDA-MB-231, MDA-MB-435 and LCC6) breast cancer cells. AIB1 positively regulated IGF-I-induced mRNA translation in both ERα(+) and ERα(-) cells. Formation of the eIF4E-4E-BP1 translational complex was altered in the AIB1 ERα(+) and ERα(-) knockdown cells, leading to a reduction in the eIF4E/4E-BP1 and eIF4G/4E-BP1 ratios. In basal and IGF-I stimulated MCF-7 and LCC6 cells, knockdown of AIB1 decreased the integrity of the cap-binding complex, reduced global IGF-I stimulated polyribosomal mRNA recruitment with a concomitant decrease in ten of the thirteen genes tested in polysome-bound mRNAs mapping to proliferation, cell cycle, survival, transcription, translation and ribosome biogenesis ontologies. Specifically, knockdown of AIB1 decreased ribosome-bound mRNA and steady-state protein levels of the transcription factors ERα and E2F1 in addition to reduced ribosome-bound mRNA of the ribosome biogenesis factor BYSL in a cell-line specific manner to regulate mRNA translation. The oncogenic transcription factor AIB1 has a novel role in the regulation of polyribosome recruitment and formation of the translational complex. Combinatorial therapies targeting IGF signaling and mRNA translation in AIB1 expressing breast cancers may have clinical benefit and warrants further investigation. Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.

Role of Reactive Mn Complexes in a Litter Decomposition Model System

NASA Astrophysics Data System (ADS)

Nico, P. S.; Keiluweit, M.; Bougoure, J.; Kleber, M.; Summering, J. A.; Maynard, J. J.; Johnson, M.; Pett-Ridge, J.

2012-12-01

The search for controls on litter decomposition rates and pathways has yet to return definitive characteristics that are both statistically robust and can be understood as part of a mechanistic or numerical model. Herein we focus on Mn, an element present in all litter that is likely an active chemical agent of decomposition. Berg and co-workers (2010) found a strong correlation between Mn concentration in litter and the magnitude of litter degradation in boreal forests, suggesting that litter decomposition proceeds more efficiently in the presence of Mn. Although there is much circumstantial evidence for the potential role of Mn in lignin decomposition, few reports exist on mechanistic details of this process. For the current work, we are guided by the hypothesis that the dependence of decomposition on Mn is due to Mn (III)-oxalate complexes act as a 'pretreatment' for structurally intact ligno-carbohydrate complexes (LCC) in fresh plant cell walls (e.g. in litter, root and wood). Manganese (III)-ligand complexes such as Mn (III)-oxalate are known to be potent oxidizers of many different organic and inorganic compounds. In the litter system, the unique property of these complexes may be that they are much smaller than exo-enzymes and therefore more easily able to penetrate LCC complexes in plant cell walls. By acting as 'diffusible oxidizers' and reacting with the organic matrix of the cell wall, these compounds can increase the porosity of fresh litter thereby facilitating access of more specific lignin- and cellulose decomposing enzymes. This possibility was investigated by reacting cell walls of single Zinnia elegans tracheary elements with Mn (III)-oxalate complexes in a continuous flow reactor. The uniformity of these individual plant cells allowed us to examine Mn (III)-induced changes in cell wall chemistry and ultrastructure on the micro-scale using fluorescence and electron microscopy as well as IR and X-ray spectromicroscopy. This presentation will discuss the chemical changes induced by reaction of Mn (III)-complexes with the Zinnia cells, the impact of such reactions on cell integrity, and potential implications for soil C cycling.

A new technique based on Artificial Bee Colony Algorithm for optimal sizing of stand-alone photovoltaic system.

PubMed

Mohamed, Ahmed F; Elarini, Mahdi M; Othman, Ahmed M

2014-05-01

One of the most recent optimization techniques applied to the optimal design of photovoltaic system to supply an isolated load demand is the Artificial Bee Colony Algorithm (ABC). The proposed methodology is applied to optimize the cost of the PV system including photovoltaic, a battery bank, a battery charger controller, and inverter. Two objective functions are proposed: the first one is the PV module output power which is to be maximized and the second one is the life cycle cost (LCC) which is to be minimized. The analysis is performed based on measured solar radiation and ambient temperature measured at Helwan city, Egypt. A comparison between ABC algorithm and Genetic Algorithm (GA) optimal results is done. Another location is selected which is Zagazig city to check the validity of ABC algorithm in any location. The ABC is more optimal than GA. The results encouraged the use of the PV systems to electrify the rural sites of Egypt.

A new technique based on Artificial Bee Colony Algorithm for optimal sizing of stand-alone photovoltaic system

PubMed Central

Mohamed, Ahmed F.; Elarini, Mahdi M.; Othman, Ahmed M.

2013-01-01

One of the most recent optimization techniques applied to the optimal design of photovoltaic system to supply an isolated load demand is the Artificial Bee Colony Algorithm (ABC). The proposed methodology is applied to optimize the cost of the PV system including photovoltaic, a battery bank, a battery charger controller, and inverter. Two objective functions are proposed: the first one is the PV module output power which is to be maximized and the second one is the life cycle cost (LCC) which is to be minimized. The analysis is performed based on measured solar radiation and ambient temperature measured at Helwan city, Egypt. A comparison between ABC algorithm and Genetic Algorithm (GA) optimal results is done. Another location is selected which is Zagazig city to check the validity of ABC algorithm in any location. The ABC is more optimal than GA. The results encouraged the use of the PV systems to electrify the rural sites of Egypt. PMID:25685507

Application of Design of Experiments and Surrogate Modeling within the NASA Advanced Concepts Office, Earth-to-Orbit Design Process

NASA Technical Reports Server (NTRS)

Zwack, Mathew R.; Dees, Patrick D.; Holt, James B.

2016-01-01

Decisions made during early conceptual design have a large impact upon the expected life-cycle cost (LCC) of a new program. It is widely accepted that up to 80% of such cost is committed during these early design phases [1]. Therefore, to help minimize LCC, decisions made during conceptual design must be based upon as much information as possible. To aid in the decision making for new launch vehicle programs, the Advanced Concepts Office (ACO) at NASA Marshall Space Flight Center (MSFC) provides rapid turnaround pre-phase A and phase A concept definition studies. The ACO team utilizes a proven set of tools to provide customers with a full vehicle mass breakdown to tertiary subsystems, preliminary structural sizing based upon worst-case flight loads, and trajectory optimization to quantify integrated vehicle performance for a given mission [2]. Although the team provides rapid turnaround for single vehicle concepts, the scope of the trade space can be limited due to analyst availability and the manpower requirements for manual execution of the analysis tools. In order to enable exploration of a broader design space, the ACO team has implemented an advanced design methods (ADM) based approach. This approach applies the concepts of design of experiments (DOE) and surrogate modeling to more exhaustively explore the trade space and provide the customer with additional design information to inform decision making. This paper will first discuss the automation of the ACO tool set, which represents a majority of the development effort. In order to fit a surrogate model within tolerable error bounds a number of DOE cases are needed. This number will scale with the number of variable parameters desired and the complexity of the system's response to those variables. For all but the smallest design spaces, the number of cases required cannot be produced within an acceptable timeframe using a manual process. Therefore, automation of the tools was a key enabler for the successful application of an ADM approach to an ACO design study. Following the overview of the tool set automation, an example problem will be given to illustrate the implementation of the ADM approach. The example problem will first cover the inclusion of ground rules and assumptions (GR&A) for a study. The GR&A are very important to the study as they determine the constraints within which a trade study can be conducted. These trades must ultimately reconcile with the customer's desired output and any anticipated "what if" questions.

Applied Meteorology Unit (AMU)

NASA Technical Reports Server (NTRS)

Bauman, William H., Jr.; Crawford, Winifred; Short, David; Barrett, Joe; Watson, Leela

2008-01-01

This report summarizes the Applied Meteorology Unit (AMU) activities for the second quarter of Fiscal Year 2008 (January - March 2008). Projects described are: (1) Peak Wind Tool for User Launch Commit Criteria (LCC), (2) Peak Wind Tool for General Forecasting, (3) Situational Lightning Climatologies for Central Florida. Phase III, (4) Volume Averaged Height Integrated Radar Reflectivity (VAHIRR), (5) Impact of Local Sensors, (6) Radar Scan Strategies for the PAFB WSR-74C Replacement and (7) WRF Wind Sensitivity Study at Edwards Air Force Base.

Army Manpower Cost System (AMCOS): Active Enlisted Force Prototype

DTIC Science & Technology

1986-03-01

cost element in both economic and budget models includes both a soldier’s Base Pay and the Service’s FICA contribu- tion at the current tax rate . a...mean base pay for the position calculated from BP T I FCAP - curret maxilum ICA payable FRATE - current FICA tax rate Tlij - total base pay distributed...Group, Santa Monica, 1982. Butler, R. and T. Neches, " HARDMAN Program Manager’s LCC Handbook: Avionics Equip- ments," D-201, The Assessment Group

Cost Structure and Life Cycle Costs for Military Systems

DTIC Science & Technology

2003-09-01

LCC 1-1 Chapter 2 – Scope of the Study 2-1 2.1 Contribution of other NATO Entities 2-1 2.2 Review of National Practises and CBS 2-1 2.3 Development...Office – NCO) gave a positive answer and designated a representative in the working group. 2.2 REVIEW OF NATIONAL PRACTISES AND CBS The two first...time preference when spending money obtained through taxation ) with the effectiveness of alternate ways of meeting an objective. By combining the

The Reliability Mandate: Optimizing the Use of Highly Reliable Parts, Materials, and Processes (PM&P) to Maximize System Component Reliability in the Life Cycle

DTIC Science & Technology

2002-06-01

projects are converted into bricks and mortar , as Figure 5 illustrates. Making major changes in LCC after projects are turned over to production is...matter experts ( SMEs ) in the parts, materials, and processes functional area. Data gathering and analysis were conducted through structured interviews...The analysis synthesized feedback and searched for collective issues from the various SMEs on managing PM&P Program requirements, the

Advanced Warheads Concepts: An Advanced Equation of State for Overdriven Detonation

DTIC Science & Technology

1991-05-01

equation of state (Jones-Wilkens Lee-Baker ( JWLB )] for high explosive detonation products. JWLB is suitable for overdriven detonation and material...In order to achieve a suitable equation of state, an appropriate equation of state form ( JWLB ) was derived. A standard explosive (octol 75/25) was...resulting equation of slate form, named Jones-Wilkens-Lcc-Baker ( JWLB ), is as follows: L ’L RiVJ .RiV+AJE + C(1.W(oH-l) -RoV X-JA^VC’V + O) The

A Novel Approach to River Basin Management that Utilizes a Multi-Day Forum to Educate Stakeholders

NASA Astrophysics Data System (ADS)

Langston, M. A.

2015-12-01

Large scale river basin management has long been a challenging task. Stakeholder involvement has often been posited as a means to provide a broad base of input and support for management decisions. This has been successful in some situations and not in others. The Desert Landscape Conservation Cooperative (LCC) has proposed a novel approach to large scale watershed management for conservation purposes by stakeholders. This approach involves conducting a multi-day stakeholder forum to gather interested parties, provide them science-based information about the watershed, and solicit their input regarding the research and management needs within the basin. Included within this forum is a Water Tournament patterned after those being developed by the U.S. Army Corps of Engineers' Institute for Water Resources. These tournaments bring stakeholders (such as the various water users, agencies, conservation organizations, and others) in small teams that develop watershed management scenarios (within appropriate constraints) that are then judged based on their merit for addressing the various issues within the basin. These tournaments serve to educate participants and to sensitize them to the perspectives of other participants. Another goal of the forum is to recruit a representative group of stakeholders who will provide guidance for further research to meet the basins management needs. The South Central Climate Science Center (SC CSC) has partnered with the Desert, Southern Rockies, Gulf Coast Prairie, Great Plains, and Gulf Coastal Plains and Ozarks LCCs to implement this approach in the Rio Grande and the Red River Basins. The LCCs are well positioned to convene stakeholders from across political boundaries and throughout these basins. The SC CSC's roles will be providing leadership, funding climate science for the effort, and evaluating the effectiveness of the forum-centered approach.

21st century projections of snowfall and winter severity across central-eastern North America

NASA Astrophysics Data System (ADS)

Notaro, M.; Lorenz, D. J.; Hoving, C.; Schummer, M.

2014-12-01

Statistically downscaled climate projections from nine global climate models (GCMs) are used to force a snow accumulation and ablation model (SNOW-17) across the central-eastern North American Landscape Conservation Cooperatives (LCCs) to develop high-resolution projections of snowfall, snow depth, and winter severity index (WSI) by the mid- and late 21st century. Here, we use projections of a cumulative WSI (CWSI) known to influence autumn-winter waterfowl migration to demonstrate the utility of SNOW-17 results. The application of statistically downscaled climate data and a snow model leads to a better representation of lake processes in the Great Lakes Basin, topographic effects in the Appalachian Mountains, and spatial patterns of climatological snowfall, compared to the original GCMs. Annual mean snowfall is simulated to decline across the region, particularly in early winter (December-January), leading to a delay in the mean onset of the snow season. Due to a warming-induced acceleration of snowmelt, the percentage loss in snow depth exceeds that of snowfall. Across the Plains and Prairie Potholes LCC and Upper Midwest and Great Lakes LCC, daily snowfall events are projected to become less common, but more intense. The greatest reductions in the number of days per year with a present snowpack are expected close to the historical position of the -5°C isotherm in DJFM, around 44°N. The CWSI is projected to decline substantially during December-January, leading to increased likelihood of delays in timing and intensity of autumn-winter waterfowl migrations.

Esthetic soft tissue management for teeth and implants.

PubMed

Fu, Jia-Hui; Su, Chuan-Yi; Wang, Hom-Lay

2012-09-01

Can newly introduced graft materials be successfully used in soft tissue augmentation around teeth and dental implants? An electronic search on the PubMed database for English articles published before March 31, 2012, was performed using the following key words: "root coverage," "soft tissue graft," "periodontal plastic surgery," "subepithelial connective graft (SCTG)," "acellular dermal matrix (ADM)," "guided tissue regeneration based root coverage (GTRC)," "recession defects," "mucogingival defects," "collagen matrix," "living cellular construct (LCC)," "mucograft," and "biologic agents." Literature featuring new soft tissue graft materials, such as ADM, collagen matrix, GTRC, and biologic agents, were included. Data showed (1) allogeneic grafts were comparable to SCTG in terms of mean complete root coverage (CRC), mean root coverage (RC), and mean amount of keratinized tissue (KT) gain; (2) xenogeneic collagen matrix was as comparable to SCTG in terms of mean amount of KT gain around teeth and dental implants but inferior in achieving RC; (3) GTRC was inferior to SCTG in terms of mean CRC and mean RC; (4) LCC was inferior to free gingival graft in terms of mean amount of KT gain but was superior in esthetics and patient satisfaction; and (5) adjunctive use of biologic agents did not exert a significant effect on mean CRC, mean RC, and mean amount of KT gain. Although these new materials do not surpass the gold standard (SCTG), they do provide improved patient satisfaction and esthetics, are available in abundance, and lead to reduced postoperative discomfort and surgical time. Copyright © 2012 Elsevier Inc. All rights reserved.

Commercial Discount Rate Estimation for Efficiency Standards Analysis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fujita, K. Sydny

2016-04-13

Underlying each of the Department of Energy's (DOE's) federal appliance and equipment standards are a set of complex analyses of the projected costs and benefits of regulation. Any new or amended standard must be designed to achieve significant additional energy conservation, provided that it is technologically feasible and economically justified (42 U.S.C. 6295(o)(2)(A)). A proposed standard is considered economically justified when its benefits exceed its burdens, as represented by the projected net present value of costs and benefits. DOE performs multiple analyses to evaluate the balance of costs and benefits of commercial appliance and equipment e efficiency standards, at themore » national and individual building or business level, each framed to capture different nuances of the complex impact of standards on the commercial end user population. The Life-Cycle Cost (LCC) analysis models the combined impact of appliance first cost and operating cost changes on a representative commercial building sample in order to identify the fraction of customers achieving LCC savings or incurring net cost at the considered efficiency levels.1 Thus, the choice of commercial discount rate value(s) used to calculate the present value of energy cost savings within the Life-Cycle Cost model implicitly plays a key role in estimating the economic impact of potential standard levels.2 This report is intended to provide a more in-depth discussion of the commercial discount rate estimation process than can be readily included in standard rulemaking Technical Support Documents (TSDs).« less

Comparative life cycle assessment and life cycle costing of four disposal scenarios for used polyethylene terephthalate bottles in Mauritius.

PubMed

Foolmaun, Rajendra Kumar; Ramjeeawon, Toolseeram

2012-09-01

The annual rise in population growth coupled with the flourishing tourism industry in Mauritius has lead to a considerable increase in the amount of solid waste generated. In parallel, the disposal of non-biodegradable wastes, especially plastic packaging and plastic bottles, has also shown a steady rise. Improper disposal of used polyethylene terephthalate (PET) bottles constitutes an eyesore to the environmental landscape and is a threat to the flourishing tourism industry. It is of utmost importance, therefore, to determine a suitable disposal method for used PET bottles which is not only environmentally efficient but is also cost effective. This study investigated the environmental impacts and the cost effectiveness of four selected disposal alternatives for used PET bottles in Mauritius. The four disposal routes investigated were: 100% landfilling; 75% incineration with energy recovery and 25% landfilling; 40% flake production (partial recycling) and 60% landfilling; and 75% flake production and 25% landfilling. Environmental impacts of the disposal alternatives were determined using ISO standardized life cycle assessment (LCA) and with the support of SimaPro 7.1 software. Cost effectiveness was determined using life cycle costing (LCC). Collected data were entered into a constructed Excel-based model to calculate the different cost categories, Net present values, damage costs and payback periods. LCA and LCC results indicated that 75% flake production and 25% landfilling was the most environmentally efficient and cost-effective disposal route for used PET bottles in Mauritius.

Pavlovian disgust conditioning as a model for contamination-based OCD: Evidence from an analogue study

PubMed Central

Armstrong, Thomas; Olatunji, Bunmi O.

2017-01-01

Pavlovian fear conditioning provides a model for anxiety-related disorders, including obsessive-compulsive disorder (OCD). However, disgust is the predominant emotional response to contamination, which is a common theme in OCD. The present study sought to identify disgust conditioning abnormalities that may underlie excessive contamination concerns relevant to OCD. Individuals high and low in contamination concern (HCC, n = 32; LCC, n = 30) completed an associative learning task in which one neutral face (conditioned stimulus; CS+) was followed by a disgusting image (unconditioned stimulus; US) and another neutral face (CS−) was unreinforced. Following this acquisition procedure, there was an extinction procedure in which both CSs were presented unreinforced. The groups did not show significant differences in discriminant responding to the CSs following acquisition. However, following extinction, the HCC group reported less reduction in their expectancy of the US following the CS+, and also reported greater disgust to the CS+, compared to the LCC group. Increased disgust to the CS+ following both acquisition and extinction was correlated with increased symptoms of contamination-based OCD and increased disgust sensitivity. Additionally, disgust sensitivity mediated group differences in disgust responding to the CS+ at acquisition and extinction. Also, failure to adjust US expectancy in response to extinction partially mediated group differences in disgust to the CS+ following extinction. Together, these findings suggest that excessive contamination concerns observed in OCD may be related to difficulty inhibiting acquired disgust, possibly due to elevated disgust sensitivity that characterizes the disorder. PMID:28391115

Small Engine Component Technology (SECT) studies

NASA Technical Reports Server (NTRS)

Meyer, P. K.; Harbour, L.

1986-01-01

A study was conducted to identify component technology requirements for small, expendable gas turbine engines that would result in substantial improvements in performance and cost by the year 2000. A subsonic, 2600 nautical mile (4815 km) strategic cruise missile mission was selected for study. A baseline (state-of-the-art) engine and missile configuration were defined to evaluate the advanced technology engines. Two advanced technology engines were configured and evaluated using advanced component efficiencies and ceramic composite materials; a 22:1 overall pressure ratio, 3.85 bypass ratio twin-spool turbofan; and an 8:1 overall pressure, 3.66 bypass ratio, single-spool recuperated turbofan with 0.85 recuperator effectiveness. Results of mission analysis indicated a reduction in fuel burn of 38 and 47 percent compared to the baseline engine when using the advanced turbofan and recuperated turbofan, respectively. While use of either advanced engine resulted in approximately a 25 percent reduction in missile size, the unit life cycle (LCC) cost reduction of 56 percent for the advanced turbofan relative to the baseline engine gave it a decisive advantage over the recuperated turbofan with 47 percent LCC reduction. An additional range improvement of 10 percent results when using a 56 percent loaded carbon slurry fuel with either engine. These results can be realized only if significant progress is attained in the fields of solid lubricated bearings, small aerodynamic component performance, composite ceramic materials and integration of slurry fuels. A technology plan outlining prospective programs in these fields is presented.

Retrieval of LAI and leaf chlorophyll content from remote sensing data by agronomy mechanism knowledge to solve the ill-posed inverse problem

NASA Astrophysics Data System (ADS)

Li, Zhenhai; Nie, Chenwei; Yang, Guijun; Xu, Xingang; Jin, Xiuliang; Gu, Xiaohe

2014-10-01

Leaf area index (LAI) and LCC, as the two most important crop growth variables, are major considerations in management decisions, agricultural planning and policy making. Estimation of canopy biophysical variables from remote sensing data was investigated using a radiative transfer model. However, the ill-posed problem is unavoidable for the unique solution of the inverse problem and the uncertainty of measurements and model assumptions. This study focused on the use of agronomy mechanism knowledge to restrict and remove the ill-posed inversion results. For this purpose, the inversion results obtained using the PROSAIL model alone (NAMK) and linked with agronomic mechanism knowledge (AMK) were compared. The results showed that AMK did not significantly improve the accuracy of LAI inversion. LAI was estimated with high accuracy, and there was no significant improvement after considering AMK. The validation results of the determination coefficient (R2) and the corresponding root mean square error (RMSE) between measured LAI and estimated LAI were 0.635 and 1.022 for NAMK, and 0.637 and 0.999 for AMK, respectively. LCC estimation was significantly improved with agronomy mechanism knowledge; the R2 and RMSE values were 0.377 and 14.495 μg cm-2 for NAMK, and 0.503 and 10.661 μg cm-2 for AMK, respectively. Results of the comparison demonstrated the need for agronomy mechanism knowledge in radiative transfer model inversion.

[Angiographic evaluation of branching pattern and anatomy of the aortic arch].

PubMed

Ergun, Onur; Tatar, İdil Güneş; Birgi, Erdem; Durmaz, Hasan Ali; Akçalar, Seray; Kurt, Aydın; Hekimoğlu, Baki

2015-04-01

The study aimed to investigate anatomical variations in branching pattern and anatomy of the aortic arch, and the prevalence of each type. Between September 2011 and November 2013, angiographic studies of 270 patients (144 male, 126 female) were analyzed retrospectively for variations in branching pattern and anatomy of the aortic arch. Patient mean age was 59.8 years (range, 13-88). Branching variations were found and divided into subtypes. Patients were also classified according to arch anatomy. Incidence of variations and types of aortic arch were statistically analysed. Analysis of the 270 patients revealed six types of branching pattern. Type I, classical pattern arch with three branches (TB, LCC, LS), was observed in 198 cases (73.3%). Type II (bovine arch), the most commonly observed variation, in which LCC originates from TB, was observed in 58 cases (21.5%). Type III, in which the left vertebral artery arises from the arch, was seen in seven cases (2.6%). Type IV, a combination of types II and III, was observed in three cases (1.1%). Type V, common origin of common carotids, LS and aberrant RS, was found in three cases (1.1%). Type VI (avian type), arch with only two branches, was observed in one case (0.4%). When patients were classified according to aortic arch anatomy, Type 1, Type 2 and Type 3 were observed in 195, 40 and 35 patients respectively. Knowledge of the variations and anatomy of the aortic arch is essential during interventional procedures and neck-thorax surgery.

Automatic liver contouring for radiotherapy treatment planning

NASA Astrophysics Data System (ADS)

Li, Dengwang; Liu, Li; Kapp, Daniel S.; Xing, Lei

2015-09-01

To develop automatic and efficient liver contouring software for planning 3D-CT and four-dimensional computed tomography (4D-CT) for application in clinical radiation therapy treatment planning systems. The algorithm comprises three steps for overcoming the challenge of similar intensities between the liver region and its surrounding tissues. First, the total variation model with the L1 norm (TV-L1), which has the characteristic of multi-scale decomposition and an edge-preserving property, is used for removing the surrounding muscles and tissues. Second, an improved level set model that contains both global and local energy functions is utilized to extract liver contour information sequentially. In the global energy function, the local correlation coefficient (LCC) is constructed based on the gray level co-occurrence matrix both of the initial liver region and the background region. The LCC can calculate the correlation of a pixel with the foreground and background regions, respectively. The LCC is combined with intensity distribution models to classify pixels during the evolutionary process of the level set based method. The obtained liver contour is used as the candidate liver region for the following step. In the third step, voxel-based texture characterization is employed for refining the liver region and obtaining the final liver contours. The proposed method was validated based on the planning CT images of a group of 25 patients undergoing radiation therapy treatment planning. These included ten lung cancer patients with normal appearing livers and ten patients with hepatocellular carcinoma or liver metastases. The method was also tested on abdominal 4D-CT images of a group of five patients with hepatocellular carcinoma or liver metastases. The false positive volume percentage, the false negative volume percentage, and the dice similarity coefficient between liver contours obtained by a developed algorithm and a current standard delineated by the expert group are on an average 2.15-2.57%, 2.96-3.23%, and 91.01-97.21% for the CT images with normal appearing livers, 2.28-3.62%, 3.15-4.33%, and 86.14-93.53% for the CT images with hepatocellular carcinoma or liver metastases, and 2.37-3.96%, 3.25-4.57%, and 82.23-89.44% for the 4D-CT images also with hepatocellular carcinoma or liver metastases, respectively. The proposed three-step method can achieve efficient automatic liver contouring for planning CT and 4D-CT images with follow-up treatment planning and should find widespread applications in future treatment planning systems.

A Comparison of Wind Speed Data from Mechanical and Ultrasonic Anemometers

NASA Technical Reports Server (NTRS)

Short, D.; Wells, L.; Merceret, F.; Roeder, W. P.

2006-01-01

This study compared the performance of mechanical and ultrasonic anemometers at the Eastern Range (ER; Kennedy Space Center and Cape Canaveral Air Force Station on Florida's Atlantic coast) and the Western Range (WR; Vandenberg Air Force Base on California's Pacific coast). Launch Weather Officers, forecasters, and Range Safety analysts need to understand the performance of wind sensors at the ER and WR for weather warnings, watches, advisories, special ground processing operations, launch pad exposure forecasts, user Launch Commit Criteria (LCC) forecasts and evaluations, and toxic dispersion support. The current ER and WR weather tower wind instruments are being changed from the current propeller-and-vane (ER) and cup-and-vane (WR) sensors to ultrasonic sensors through the Range Standardization and Automation (RSA) program. The differences between mechanical and ultrasonic techniques have been found to cause differences in the statistics of peak wind speed in previous studies. The 45th Weather Squadron (45 WS) and the 30th Weather Squadron (30 WS) requested the Applied Meteorology Unit (AMU) to compare data between RSA and current sensors to determine if there are significant differences. Approximately 3 weeks of Legacy and RSA wind data from each range were used in the study, archived during May and June 2005. The ER data spanned the full diurnal cycle, while the WR data was confined to 1000-1600 local time. The sample of 1-minute data from numerous levels on 5 different towers on each range totaled more than 500,000 minutes of data (482,979 minutes of data after quality control). The 10 towers were instrumented at several levels, ranging from 12 ft to 492 ft above ground level. The RSA sensors were collocated at the same vertical levels as the present sensors and typically within 15 ft horizontally of each another. Data from a total of 53 RSA ultrasonic sensors, collocated with present sensors were compared. The 1-minute average wind speed/direction and the 1-second peak wind speed/direction were compared.

Applied Meteorology Unit (AMU) Quarterly Report - Fourth Quarter FY-09

NASA Technical Reports Server (NTRS)

Bauman, William; Crawford, Winifred; Barrett, Joe; Watson, Leela; Wheeler, Mark

2009-01-01

This report summarizes the Applied Meteorology Unit (AMU) activities for the fourth quarter of Fiscal Year 2009 (July - September 2009). Tasks reports include: (1) Peak Wind Tool for User Launch Commit Criteria (LCC), (2) Objective Lightning Probability Tool. Phase III, (3) Peak Wind Tool for General Forecasting. Phase II, (4) Update and Maintain Advanced Regional Prediction System (ARPS) Data Analysis System (ADAS), (5) Verify MesoNAM Performance (6) develop a Graphical User Interface to update selected parameters for the Hybrid Single-Particle Lagrangian Integrated Trajectory (HYSPLlT)

Applied Meteorology Unit (AMU) Quarterly Report Third Quarter FY-08

NASA Technical Reports Server (NTRS)

Bauman, William; Crawford, Winifred; Barrett, Joe; Watson, Leela; Dreher, Joseph

2008-01-01

This report summarizes the Applied Meteorology Unit (AMU) activities for the third quarter of Fiscal Year 2008 (April - June 2008). Tasks reported on are: Peak Wind Tool for User Launch Commit Criteria (LCC), Anvil Forecast Tool in AWIPS Phase II, Completion of the Edward Air Force Base (EAFB) Statistical Guidance Wind Tool, Volume Averaged Height Integ rated Radar Reflectivity (VAHIRR), Impact of Local Sensors, Radar Scan Strategies for the PAFB WSR-74C Replacement, VAHIRR Cost Benefit Analysis, and WRF Wind Sensitivity Study at Edwards Air Force Base

Contingency power concepts for helicopter turboshaft engine

NASA Technical Reports Server (NTRS)

Hirschkron, R.; Davis, R. H.; Goldstein, D. N.; Haynes, J. F.; Gauntner, J. W.

1984-01-01

Twin helicopter engines are often sized by power requirement of safe mission completion after the failure of one of the two engines. This study was undertaken for NASA Lewis by General Electric Co. to evaluate the merits of special design features to provide a 2-1/2 minute Contingency Power rating, permitting an engine size reduction. The merits of water injection, cooling flow modulation, throttle push and an auxiliary power plant were evaluated using military life cycle cost (LCC) and commercial helicopter direct operating cost (DOC) merit factors in a rubber engine/rubber aircraft scenario.

Analysis of environmental factors impacting the life cycle cost analysis of conventional and fuel cell/battery-powered passenger vehicles. Final report

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

This report presents the results of the further developments and testing of the Life Cycle Cost (LCC) Model previously developed by Engineering Systems Management, Inc. (ESM) on behalf of the U.S. Department of Energy (DOE) under contract No. DE-AC02-91CH10491. The Model incorporates specific analytical relationships and cost/performance data relevant to internal combustion engine (ICE) powered vehicles, battery powered electric vehicles (BPEVs), and fuel cell/battery-powered electric vehicles (FCEVs).

The General Aviation Dynamics Model Volume III. Systems Manual.

DTIC Science & Technology

1979-07-01

SEXi and SEX2 or in some other way, as desired by the user. The descriptors of an index are used as stubs (row descriptors) and/or spanners (column...BAR A produces the following plot [7 SUWPLO~TS DATASET A, 1970 TO 1975 8+ .QL xx YXXMX 4+ MXUXX IDLMXX XXXXXX * yyx :mx0(c XXX *m XXXX XX XXXXX XMxK...VXXXCK 2+ mm~ XXXXX MMXXX I~CUM LXXMXx *X2~ XXX xXX =uXX ImXXXLMX * xx Iac lxxno IXxc= )LccIQX * XX= )LXXX ILMKXX X.XXXX IXXCX SHOW

Identification of the Properties of Gum Arabic Used as a Binder in 7.62-mm Ammunition Primers

DTIC Science & Technology

2010-06-01

Solution - LCC Testing (ATK Task 700) 51 Cartridge - Ballistic Testing (ATK Task 800) 51 ATK Elemental Analysis 52 Moisture Loss and Friability...Hummel sample 7 3 SDT summary for Quadra sample 8 4 Particle size analysis summary for gum arabic samples 9 5 SEM images of Colony gum arabic at 230x...strengths 21 16 Color analysis : Colony after 5.0 hrs 23 17 Color analysis : Hummel after 5.0 hrs 23 18 Color analysis : Brenntag after 5.0 hrs 23 19 Gel

Total Life Cycle Management for the Special Operations Craft Riverine

DTIC Science & Technology

2012-12-04

Logistics Command in Albany, GA. He is married to the former Catherine Simmons of Blackwater , Australia, and they have a daughter, two sons, and...those of the author and do not reflect the official policy position of the Navy , the Department of Defense, or the Federal Government. = ^Åèìáëáíáçå...consumption. If the Navy sets an upper limit on the LCC of a new conventionally powered vessel, fuel consumption over the lifetime = ^Åèìáëáíáçå=oÉëÉ

Analysis of the Proposed Use of Minuteman Missile Combat Crew Members as Maintenance Extenders.

DTIC Science & Technology

1984-09-01

which was actually closed and secured after duty hours by a large bank vault-type door. D022. Acronym for the Plans and Intelligence Division, a DO...was secured with a bank vault-type door. 12 p I I I Maintenance I I----lt --u ----ty I -- -- I Training I I Mantenance I I I OHMS I FMMS...adding more duties? Are you crazy? -. OHMS Shop Chiefs .. every time I go down to the LCC the MCCMs are sitting around in their pajamas reading dirty

Somatic alterations of the serine/threonine kinase LKB1 gene in squamous cell (SCC) and large cell (LCC) lung carcinoma.

PubMed

Strazisar, Mojca; Mlakar, Vid; Rott, Tomaz; Glavac, Damjan

2009-05-01

Somatic LKB1 serine/threonine kinase alterations are rare in sporadic cancers, with the exception lung adenocarcinoma, but no mutations in squamous cell or large cell primary carcinoma were discovered. We screened the LKB1 gene in 129 primary nonsmall cell lung carcinomas, adjacent healthy lung tissue, and control blood samples. Forty-five percent of nonsmall cell lung tumors harbored either intron or exon alterations. We identified R86G, F354L, Y272Y and three polymorphisms: 290+36G/T, 386+156G/T, and 862+145C/T (novel). R86G (novel) and F354L mutations were found in six squamous cell carcinomas and three large cell cancer carcinomas, but not in the adjacent healthy tissue or controls samples. The F354L mutation was found in advanced squamous cell carcinomas with elevated COX-2 expression, rare P53, and no K-RAS mutation. Results indicate that the LKB1 gene is changed in a certain proportion of nonsmall cell lung tumors, predominately in advanced squamous lung carcinoma. Inactivation of the gene takes place via the C-terminal domain and could be related to mechanisms influencing tumor initiation, differentiation, and metastasis.

Proposed Trace Gas Measurements Over the Western United States for TROPOMI Validation

NASA Technical Reports Server (NTRS)

Parworth, Caroline L.; Marrero, Josette E.; Yates, Emma L.; Ryoo, Ju-Mee; Iraci, Laura T.

2018-01-01

The Alpha Jet Atmospheric eXperiment (AJAX), located in the Bay Area of California, is a joint effort between NASA Ames Research Center and H211, LCC. AJAX makes in-situ airborne measurements of trace gases 2-4 times per month, resulting in over 216 flights since 2011. Current measurements include ozone (O3), carbon dioxide (CO2), methane (CH4), water (H2O), formaldehyde (HCHO), and meteorological measurements (i.e., ambient pressure, temperature, and 3D winds). Currently, the AJAX team is working to incorporate nitrogen dioxide (NO2) measurements with a Cavity Attenuated Phase Shift Spectrometer (CAPS). Successful science flights coincident with satellite overpasses have been performed since 2011 by the Alpha Jet, with more than 40 flights under the Greenhouse Observing SATellite (GOSAT) and several flights under the Orbiting Carbon Observatory-2 (OCO-2). Results from these flights, which have covered a range of different surfaces and seasonal conditions, will be presented. In-situ vertical profiles of O3, CO2, CH4, H2O, HCHO, and NO2 from the surface to 28,000 feet made by AJAX will also be valuable for satellite validation of data products obtained from the TROPOspheric Montoring Instrument (TROPOMI). TROPOMI is on board the Copernicus Sentinel-5 precursor (S5p) satellite, with level 2 products including O3, CO, CH4, HCHO, NO2, and aerosols.

Transformer Efficiency Assessment - Okinawa, Japan

DOE Office of Scientific and Technical Information (OSTI.GOV)

Thomas L. Baldwin; Robert J. Turk; Kurt S. Myers

The US Army Engineering & Support Center, Huntsville (USAESCH), and the US Marine Corps Base (MCB), Okinawa, Japan retained Idaho National Laboratory (INL) to conduct a Transformer Efficiency Assessment of “key” transformers located at multiple military bases in Okinawa, Japan. The purpose of this assessment is to support the Marine Corps Base, Okinawa in evaluating medium voltage distribution transformers for potential efficiency upgrades. The original scope of work included the MCB providing actual transformer nameplate data, manufacturer’s factory test sheets, electrical system data (kWh), demand data (kWd), power factor data, and electricity cost data. Unfortunately, the MCB’s actual data ismore » not available and therefore making it necessary to de-scope the original assessment. Note: Any similar nameplate data, photos of similar transformer nameplates, and basic electrical details from one-line drawings (provided by MCB) are not a replacement for actual load loss test data. It is recommended that load measurements are performed on the high and low sides of transformers to better quantify actual load losses, demand data, and power factor data. We also recommend that actual data, when available, be inserted by MCB Okinawa where assumptions have been made and then the LCC analysis updated. This report covers a generalized assessment of modern U.S. transformers in a three level efficiency category, Low-Level efficiency, Medium-Level efficiency, and High-Level efficiency.« less

Transformer Efficiency Assessment - Okinawa, Japan

DOE Office of Scientific and Technical Information (OSTI.GOV)

Thomas L. Baldwin; Robert J. Turk; Kurt S. Myers

2012-05-01

The US Army Engineering & Support Center, Huntsville (USAESCH), and the US Marine Corps Base (MCB), Okinawa, Japan retained Idaho National Laboratory (INL) to conduct a Transformer Efficiency Assessment of “key” transformers located at multiple military bases in Okinawa, Japan. The purpose of this assessment is to support the Marine Corps Base, Okinawa in evaluating medium voltage distribution transformers for potential efficiency upgrades. The original scope of work included the MCB providing actual transformer nameplate data, manufacturer’s factory test sheets, electrical system data (kWh), demand data (kWd), power factor data, and electricity cost data. Unfortunately, the MCB’s actual data ismore » not available and therefore making it necessary to de-scope the original assessment. Note: Any similar nameplate data, photos of similar transformer nameplates, and basic electrical details from one-line drawings (provided by MCB) are not a replacement for actual load loss test data. It is recommended that load measurements are performed on the high and low sides of transformers to better quantify actual load losses, demand data, and power factor data. We also recommend that actual data, when available, be inserted by MCB Okinawa where assumptions have been made and then the LCC analysis updated. This report covers a generalized assessment of modern U.S. transformers in a three level efficiency category, Low-Level efficiency, Medium-Level efficiency, and High-Level efficiency.« less

Estimating the Life Cycle Cost of Space Systems

NASA Technical Reports Server (NTRS)

Jones, Harry W.

2015-01-01

A space system's Life Cycle Cost (LCC) includes design and development, launch and emplacement, and operations and maintenance. Each of these cost factors is usually estimated separately. NASA uses three different parametric models for the design and development cost of crewed space systems; the commercial PRICE-H space hardware cost model, the NASA-Air Force Cost Model (NAFCOM), and the Advanced Missions Cost Model (AMCM). System mass is an important parameter in all three models. System mass also determines the launch and emplacement cost, which directly depends on the cost per kilogram to launch mass to Low Earth Orbit (LEO). The launch and emplacement cost is the cost to launch to LEO the system itself and also the rockets, propellant, and lander needed to emplace it. The ratio of the total launch mass to payload mass depends on the mission scenario and destination. The operations and maintenance costs include any material and spares provided, the ground control crew, and sustaining engineering. The Mission Operations Cost Model (MOCM) estimates these costs as a percentage of the system development cost per year.

Ultrasound-intensified laccase production from Trametes versicolor.

PubMed

Wang, Feng; Ma, An-Zhou; Guo, Chen; Zhuang, Guo-Qiang; Liu, Chun-Zhao

2013-01-01

An efficient intermittent ultrasonic treatment strategy was developed to improve laccase production from Trametes versicolor mycelia cultures. The optimized strategy consisted of exposing 2-day-old mycelia cultures to 5-min ultrasonic treatments for two times with a 12-h interval at the fixed ultrasonic power and frequency (120 W, 40 kHz). After 5 days of culture, this strategy produced the highest extracellular laccase activity of 588.9 U/L among all treatments tested which was 1.8-fold greater than the control without ultrasound treatment. The ultrasonic treatment resulted in a higher pellet porosity that facilitated the mass transfer of nutrients and metabolites from the pellets to the surrounding liquid. Furthermore, the ultrasonic treatment induced the expression of the laccase gene (lcc), which correlated with a sharp increase in both extracellular and intracellular laccase activity. This is the first study to find positive effects of ultrasound on gene expression in fungal cells. These results provide a basis for understanding the stimulation of metabolite production and process intensification by ultrasonic treatment in filamentous fungal culture. Copyright © 2012 Elsevier B.V. All rights reserved.

Laccase detoxification mediates the nutritional alliance between leaf-cutting ants and fungus-garden symbionts

PubMed Central

De Fine Licht, Henrik H.; Schiøtt, Morten; Rogowska-Wrzesinska, Adelina; Nygaard, Sanne; Roepstorff, Peter; Boomsma, Jacobus J.

2013-01-01

Leaf-cutting ants combine large-scale herbivory with fungus farming to sustain advanced societies. Their stratified colonies are major evolutionary achievements and serious agricultural pests, but the crucial adaptations that allowed this mutualism to become the prime herbivorous component of neotropical ecosystems has remained elusive. Here we show how coevolutionary adaptation of a specific enzyme in the fungal symbiont has helped leaf-cutting ants overcome plant defensive phenolic compounds. We identify nine putative laccase-coding genes in the fungal genome of Leucocoprinus gongylophorus cultivated by the leaf-cutting ant Acromyrmex echinatior. One of these laccases (LgLcc1) is highly expressed in the specialized hyphal tips (gongylidia) that the ants preferentially eat, and we confirm that these ingested laccase molecules pass through the ant guts and remain active when defecated on the leaf pulp that the ants add to their gardens. This accurate deposition ensures that laccase activity is highest where new leaf material enters the fungus garden, but where fungal mycelium is too sparse to produce extracellular enzymes in sufficient quantities to detoxify phenolic compounds. Phylogenetic analysis of LgLcc1 ortholog sequences from symbiotic and free-living fungi revealed significant positive selection in the ancestral lineage that gave rise to the gongylidia-producing symbionts of leaf-cutting ants and their non–leaf-cutting ant sister group. Our results are consistent with fungal preadaptation and subsequent modification of a particular laccase enzyme for the detoxification of secondary plant compounds during the transition to active herbivory in the ancestor of leaf-cutting ants between 8 and 12 Mya. PMID:23267060

Laccase detoxification mediates the nutritional alliance between leaf-cutting ants and fungus-garden symbionts.

PubMed

De Fine Licht, Henrik H; Schiøtt, Morten; Rogowska-Wrzesinska, Adelina; Nygaard, Sanne; Roepstorff, Peter; Boomsma, Jacobus J

2013-01-08

Leaf-cutting ants combine large-scale herbivory with fungus farming to sustain advanced societies. Their stratified colonies are major evolutionary achievements and serious agricultural pests, but the crucial adaptations that allowed this mutualism to become the prime herbivorous component of neotropical ecosystems has remained elusive. Here we show how coevolutionary adaptation of a specific enzyme in the fungal symbiont has helped leaf-cutting ants overcome plant defensive phenolic compounds. We identify nine putative laccase-coding genes in the fungal genome of Leucocoprinus gongylophorus cultivated by the leaf-cutting ant Acromyrmex echinatior. One of these laccases (LgLcc1) is highly expressed in the specialized hyphal tips (gongylidia) that the ants preferentially eat, and we confirm that these ingested laccase molecules pass through the ant guts and remain active when defecated on the leaf pulp that the ants add to their gardens. This accurate deposition ensures that laccase activity is highest where new leaf material enters the fungus garden, but where fungal mycelium is too sparse to produce extracellular enzymes in sufficient quantities to detoxify phenolic compounds. Phylogenetic analysis of LgLcc1 ortholog sequences from symbiotic and free-living fungi revealed significant positive selection in the ancestral lineage that gave rise to the gongylidia-producing symbionts of leaf-cutting ants and their non-leaf-cutting ant sister group. Our results are consistent with fungal preadaptation and subsequent modification of a particular laccase enzyme for the detoxification of secondary plant compounds during the transition to active herbivory in the ancestor of leaf-cutting ants between 8 and 12 Mya.

The New Weather Radar for America's Space Program in Florida: A Temperature Profile Adaptive Scan Strategy

NASA Technical Reports Server (NTRS)

Carey, L. D.; Petersen, W. A.; Deierling, W.; Roeder, W. P.

2009-01-01

A new weather radar is being acquired for use in support of America s space program at Cape Canaveral Air Force Station, NASA Kennedy Space Center, and Patrick AFB on the east coast of central Florida. This new radar replaces the modified WSR-74C at Patrick AFB that has been in use since 1984. The new radar is a Radtec TDR 43-250, which has Doppler and dual polarization capability. A new fixed scan strategy was designed to best support the space program. The fixed scan strategy represents a complex compromise between many competing factors and relies on climatological heights of various temperatures that are important for improved lightning forecasting and evaluation of Lightning Launch Commit Criteria (LCC), which are the weather rules to avoid lightning strikes to in-flight rockets. The 0 C to -20 C layer is vital since most generation of electric charge occurs within it and so it is critical in evaluating Lightning LCC and in forecasting lightning. These are two of the most important duties of 45 WS. While the fixed scan strategy that covers most of the climatological variation of the 0 C to -20 C levels with high resolution ensures that these critical temperatures are well covered most of the time, it also means that on any particular day the radar is spending precious time scanning at angles covering less important heights. The goal of this project is to develop a user-friendly, Interactive Data Language (IDL) computer program that will automatically generate optimized radar scan strategies that adapt to user input of the temperature profile and other important parameters. By using only the required scan angles output by the temperature profile adaptive scan strategy program, faster update times for volume scans and/or collection of more samples per gate for better data quality is possible, while maintaining high resolution at the critical temperature levels. The temperature profile adaptive technique will also take into account earth curvature and refraction when geo-locating the radar beam (i.e., beam height and arc distance), including non-standard refraction based on the user-input temperature profile. In addition to temperature profile adaptivity, this paper will also summarize the other requirements for this scan strategy program such as detection of low-level boundaries, detection of anvil clouds, reducing the Cone Of Silence, and allowing for times when deep convective clouds will not occur. The adaptive technique will be carefully compared to and benchmarked against the new fixed scan strategy. Specific environmental scenarios in which the adaptive scan strategy is able to optimize and improve coverage and resolution at critical heights, scan time, and/or sample numbers relative to the fixed scan strategy will be presented.

Applied Meteorology Unit (AMU)

NASA Technical Reports Server (NTRS)

Bauman, William; Crawford, Winifred; Barrett, Joe; Watson, Leela; Wheeler, Mark

2010-01-01

This report summarizes the Applied Meteorology Unit (AMU) activities for the first quarter of Fiscal Year 2010 (October - December 2009). A detailed project schedule is included in the Appendix. Included tasks are: (1) Peak Wind Tool for User Launch Commit Criteria (LCC), (2) Objective Lightning Probability Tool, Phase III, (3) Peak Wind Tool for General Forecasting, Phase II, (4) Upgrade Summer Severe Weather Tool in Meteorological Interactive Data Display System (MIDDS), (5) Advanced Regional Prediction System (ARPS) Data Analysis System (ADAS) Update and Maintainability, (5) Verify 12-km resolution North American Model (MesoNAM) Performance, and (5) Hybrid Single-Particle Lagrangian Integrated Trajectory (HYSPLIT) Graphical User Interface.

Rotorcraft contingency power study

NASA Technical Reports Server (NTRS)

Hirschkron, R.; Haynes, J. F.; Goldstein, D. N.; Davis, R. H.

1984-01-01

Twin helicopter engines are often sized by the power requirement of a safe mission completion after the failure of one of the two engines. This study was undertaken for NASA Lewis by General Electric Co. to evaluate the merits of special design features to provide a 2-1/2 Contingency Power rating, permitting an engine size reduction. The merits of water injection, turbine cooling airflow modulation, throttle push, and a propellant auxiliary power plant were evaluated using military Life Cycle Cost (LCC) and commercial helicopter Direct Operating Cost (DOC) merit factors in a rubber engine and a rubber aircraft scenario.

Financing Humanitarian Assistance and Disaster Response: The Case of the Tohoku Earthquake and Operation Tomodachi

DTIC Science & Technology

2012-05-16

Fred Dini, SC, USN, JSF Japan Comptroller, May 2011 Explosion at Unit 4 at Fukushima Daiichi Stand-off area set at 30km radius USS Tortuga offloads...X X X X X X X X X X X X X X X X X X X X X O USNS TORTUGA LSD U U U X X X X X X X X X X X X X X X X X X X X X X X X X O USNS BLUE RIDGE LCC U U U U U

Life Cycle Assessment and Costing Methods for Device Procurement: Comparing Reusable and Single-Use Disposable Laryngoscopes.

PubMed

Sherman, Jodi D; Raibley, Lewis A; Eckelman, Matthew J

2018-01-09

Traditional medical device procurement criteria include efficacy and safety, ease of use and handling, and procurement costs. However, little information is available about life cycle environmental impacts of the production, use, and disposal of medical devices, or about costs incurred after purchase. Reusable and disposable laryngoscopes are of current interest to anesthesiologists. Facing mounting pressure to quickly meet or exceed conflicting infection prevention guidelines and oversight body recommendations, many institutions may be electively switching to single-use disposable (SUD) rigid laryngoscopes or overcleaning reusables, potentially increasing both costs and waste generation. This study provides quantitative comparisons of environmental impacts and total cost of ownership among laryngoscope options, which can aid procurement decision making to benefit facilities and public health. We describe cradle-to-grave life cycle assessment (LCA) and life cycle costing (LCC) methods and apply these to reusable and SUD metal and plastic laryngoscope handles and tongue blade alternatives at Yale-New Haven Hospital (YNHH). The US Environmental Protection Agency's Tool for the Reduction and Assessment of Chemical and other environmental Impacts (TRACI) life cycle impact assessment method was used to model environmental impacts of greenhouse gases and other pollutant emissions. The SUD plastic handle generates an estimated 16-18 times more life cycle carbon dioxide equivalents (CO2-eq) than traditional low-level disinfection of the reusable steel handle. The SUD plastic tongue blade generates an estimated 5-6 times more CO2-eq than the reusable steel blade treated with high-level disinfection. SUD metal components generated much higher emissions than all alternatives. Both the SUD handle and SUD blade increased life cycle costs compared to the various reusable cleaning scenarios at YNHH. When extrapolated over 1 year (60,000 intubations), estimated costs increased between $495,000 and $604,000 for SUD handles and between $180,000 and $265,000 for SUD blades, compared to reusables, depending on cleaning scenario and assuming 4000 (rated) uses. Considering device attrition, reusable handles would be more economical than SUDs if they last through 4-5 uses, and reusable blades 5-7 uses, before loss. LCA and LCC are feasible methods to ease interpretation of environmental impacts and facility costs when weighing device procurement options. While management practices vary between institutions, all standard methods of cleaning were evaluated and sensitivity analyses performed so that results are widely applicable. For YNHH, the reusable options presented a considerable cost advantage, in addition to offering a better option environmentally. Avoiding overcleaning reusable laryngoscope handles and blades is desirable from an environmental perspective. Costs may vary between facilities, and LCC methodology demonstrates the importance of time-motion labor analysis when comparing reusable and disposable device options.

Launch Control System Software Development System Automation Testing

NASA Technical Reports Server (NTRS)

Hwang, Andrew

2017-01-01

The Spaceport Command and Control System (SCCS) is the National Aeronautics and Space Administration's (NASA) launch control system for the Orion capsule and Space Launch System, the next generation manned rocket currently in development. This system requires high quality testing that will measure and test the capabilities of the system. For the past two years, the Exploration and Operations Division at Kennedy Space Center (KSC) has assigned a group including interns and full-time engineers to develop automated tests to save the project time and money. The team worked on automating the testing process for the SCCS GUI that would use streamed simulated data from the testing servers to produce data, plots, statuses, etc. to the GUI. The software used to develop automated tests included an automated testing framework and an automation library. The automated testing framework has a tabular-style syntax, which means the functionality of a line of code must have the appropriate number of tabs for the line to function as intended. The header section contains either paths to custom resources or the names of libraries being used. The automation library contains functionality to automate anything that appears on a desired screen with the use of image recognition software to detect and control GUI components. The data section contains any data values strictly created for the current testing file. The body section holds the tests that are being run. The function section can include any number of functions that may be used by the current testing file or any other file that resources it. The resources and body section are required for all test files; the data and function sections can be left empty if the data values and functions being used are from a resourced library or another file. To help equip the automation team with better tools, the Project Lead of the Automated Testing Team, Jason Kapusta, assigned the task to install and train an optical character recognition (OCR) tool to Brandon Echols, a fellow intern, and I. The purpose of the OCR tool is to analyze an image and find the coordinates of any group of text. Some issues that arose while installing the OCR tool included the absence of certain libraries needed to train the tool and an outdated software version. We eventually resolved the issues and successfully installed the OCR tool. Training the tool required many images and different fonts and sizes, but in the end the tool learned to accurately decipher the text in the images and their coordinates. The OCR tool produced a file that contained significant metadata for each section of text, but only the text and coordinates of the text was required for our purpose. The team made a script to parse the information we wanted from the OCR file to a different file that would be used by automation functions within the automated framework. Since a majority of development and testing for the automated test cases for the GUI in question has been done using live simulated data on the workstations at the Launch Control Center (LCC), a large amount of progress has been made. As of this writing, about 60% of all of automated testing has been implemented. Additionally, the OCR tool will help make our automated tests more robust due to the tool's text recognition being highly scalable to different text fonts and text sizes. Soon we will have the whole test system automated, allowing for more full-time engineers working on development projects.

Changes in land cover and vegetation carbon stocks in Andalusia, Southern Spain (1956-2007).

PubMed

Muñoz-Rojas, M; De la Rosa, D; Zavala, L M; Jordán, A; Anaya-Romero, M

2011-06-15

Land use has significantly changed during the recent decades at global and local scale, while the importance of ecosystems as sources/sinks of C has been highlighted, emphasizing the global impact of land use changes. Land use changes can increase C loss rates which are extremely difficult to reverse, in the short term, opposite to organic carbon (OC) which accumulates in soil in the long-term. The aim of this research is to improve and test methodologies to assess land cover change (LCC) dynamics and temporal and spatial variability in C stored in vegetation at a wide scale. LCCs between 1956 and 2007 in Andalusia (Southern Spain) were selected for this pilot study, assessed by comparison of spatial data from 1956 to 2007 and were reclassified following land cover flows (LCFs) reported in major areas in Europe. Carbon vegetation densities were related to land cover, and C vegetation stocks for 1956 and 2007 were calculated by multiplying C density for each land cover class with land cover areas. The study area has supported important changes during the studied period with significant consequences for vegetation C stocks, mainly due to afforestation and intensification of agriculture, resulting in a total vegetation C stock of 156.08Tg in 2007, with an increase of 17.24Tg since 1956. This study demonstrates the importance of LCC for C sequestration in vegetation from Mediterranean areas, highlighting possible directions for management policies in order to mitigate climate change as well as promoting land conservation. The methodologies and information generated in this project will be a useful basis for designing land management strategies helpful for decision makers. Copyright © 2011 Elsevier B.V. All rights reserved.

Natural history of major complications in hepatitis C virus-related cirrhosis evaluated by per-rectal portal scintigraphy

PubMed Central

Kawamura, Etsushi; Habu, Daiki; Hayashi, Takehiro; Oe, Ai; Kotani, Jin; Ishizu, Hirotaka; Torii, Kenji; Kawabe, Joji; Fukushima, Wakaba; Tanaka, Takashi; Nishiguchi, Shuhei; Shiomi, Susumu

2005-01-01

AIM: To examine the correlation between the porto-systemic hypertension evaluated by portal shunt index (PSI) and life-threatening complications, including hepatocellular carcinoma (HCC), liver failure (Child-Pugh stage progression), and esophagogastric varices. METHODS: Two hundred and twelve consecutive subjects with HCV-related cirrhosis (LC-C) underwent per-rectal portal scintigraphy. They were allocated into three groups according to their PSI: group I, PSI ≤ 10%; group II, 10%

Construction and Characterization of an in-vivo Linear Covalently Closed DNA Vector Production System

PubMed Central

2012-01-01

Background While safer than their viral counterparts, conventional non-viral gene delivery DNA vectors offer a limited safety profile. They often result in the delivery of unwanted prokaryotic sequences, antibiotic resistance genes, and the bacterial origins of replication to the target, which may lead to the stimulation of unwanted immunological responses due to their chimeric DNA composition. Such vectors may also impart the potential for chromosomal integration, thus potentiating oncogenesis. We sought to engineer an in vivo system for the quick and simple production of safer DNA vector alternatives that were devoid of non-transgene bacterial sequences and would lethally disrupt the host chromosome in the event of an unwanted vector integration event. Results We constructed a parent eukaryotic expression vector possessing a specialized manufactured multi-target site called “Super Sequence”, and engineered E. coli cells (R-cell) that conditionally produce phage-derived recombinase Tel (PY54), TelN (N15), or Cre (P1). Passage of the parent plasmid vector through R-cells under optimized conditions, resulted in rapid, efficient, and one step in vivo generation of mini lcc—linear covalently closed (Tel/TelN-cell), or mini ccc—circular covalently closed (Cre-cell), DNA constructs, separated from the backbone plasmid DNA. Site-specific integration of lcc plasmids into the host chromosome resulted in chromosomal disruption and 105 fold lower viability than that seen with the ccc counterpart. Conclusion We offer a high efficiency mini DNA vector production system that confers simple, rapid and scalable in vivo production of mini lcc DNA vectors that possess all the benefits of “minicircle” DNA vectors and virtually eliminate the potential for undesirable vector integration events. PMID:23216697

Comparison of four statistical and machine learning methods for crash severity prediction.

PubMed

Iranitalab, Amirfarrokh; Khattak, Aemal

2017-11-01

Crash severity prediction models enable different agencies to predict the severity of a reported crash with unknown severity or the severity of crashes that may be expected to occur sometime in the future. This paper had three main objectives: comparison of the performance of four statistical and machine learning methods including Multinomial Logit (MNL), Nearest Neighbor Classification (NNC), Support Vector Machines (SVM) and Random Forests (RF), in predicting traffic crash severity; developing a crash costs-based approach for comparison of crash severity prediction methods; and investigating the effects of data clustering methods comprising K-means Clustering (KC) and Latent Class Clustering (LCC), on the performance of crash severity prediction models. The 2012-2015 reported crash data from Nebraska, United States was obtained and two-vehicle crashes were extracted as the analysis data. The dataset was split into training/estimation (2012-2014) and validation (2015) subsets. The four prediction methods were trained/estimated using the training/estimation dataset and the correct prediction rates for each crash severity level, overall correct prediction rate and a proposed crash costs-based accuracy measure were obtained for the validation dataset. The correct prediction rates and the proposed approach showed NNC had the best prediction performance in overall and in more severe crashes. RF and SVM had the next two sufficient performances and MNL was the weakest method. Data clustering did not affect the prediction results of SVM, but KC improved the prediction performance of MNL, NNC and RF, while LCC caused improvement in MNL and RF but weakened the performance of NNC. Overall correct prediction rate had almost the exact opposite results compared to the proposed approach, showing that neglecting the crash costs can lead to misjudgment in choosing the right prediction method. Copyright © 2017 Elsevier Ltd. All rights reserved.

Hydroxychloroquine inhibits autophagy to potentiate antiestrogen responsiveness in ER+ breast cancer

PubMed Central

Cook, Katherine L.; Wärri, Anni; Soto-Pantoja, David R.; Clarke, Pamela A.G.; Cruz, M. Idalia; Zwart, Alan; Clarke, Robert

2014-01-01

Purpose Estrogen receptor-α (ERα) targeted therapies including tamoxifen (TAM) or Faslodex (ICI) are used to treat ER+ breast cancers. Up to 50% of tumors will acquire resistance to these interventions. Autophagy has been implicated as a major driver of antiestrogen resistance. We have explored the ability of hydroxychloroquine (HCQ), which inhibits autophagy, to affect antiestrogen responsiveness. Experimental Design TAM-resistant MCF7-RR and ICI-resistant/TAM cross-resistant LCC9 ER+ breast cancer cells were injected into mammary fat pads of female athymic mice and treated with TAM and/or ICI in combination with oral low-dose HCQ. Results We show HCQ can increase antiestrogen responsiveness in MCF7-RR and LCC9 cells and tumors, likely through the inhibition of autophagy. However, the combination of ICI+HCQ was less effective than HCQ alone in vivo, unlike the TAM+HCQ combination. Antiestrogen treatment stimulated angiogenesis in tumors but did not prevent HCQ effectiveness. The lower efficacy of ICI+HCQ was associated with ICI effects on cell-mediated immunity within the tumor microenvironment. The mouse chemokine KC (CXCL1) and IFNγ were differentially regulated by both TAM and ICI treatments, suggesting a possible effect on macrophage development/activity. Consistent with these observations, TAM+HCQ treatment increased tumor CD68+ cells infiltration, whereas ICI and ICI+HCQ reduced peripheral tumor macrophage content. Moreover, macrophage elimination of breast cancer target cells in vitro was reduced following exposure to ICI. Conclusion HCQ restores antiestrogen sensitivity to resistant tumors. Moreover, the beneficial combination of TAM+HCQ suggests a positive outcome for ongoing neoadjuvant clinical trials using this combination for the treatment of ER+ ductal carcinoma in situ lesions. PMID:24928945

DOE Office of Scientific and Technical Information (OSTI.GOV)

Doug Cathro

The Lake Charles CCS Project is a large-scale industrial carbon capture and sequestration (CCS) project which will demonstrate advanced technologies that capture and sequester carbon dioxide (CO{sub 2}) emissions from industrial sources into underground formations. Specifically the Lake Charles CCS Project will accelerate commercialization of large-scale CO{sub 2} storage from industrial sources by leveraging synergy between a proposed petroleum coke to chemicals plant (the LCC Gasification Project) and the largest integrated anthropogenic CO{sub 2} capture, transport, and monitored sequestration program in the U.S. Gulf Coast Region. The Lake Charles CCS Project will promote the expansion of EOR in Texas andmore » Louisiana and supply greater energy security by expanding domestic energy supplies. The capture, compression, pipeline, injection, and monitoring infrastructure will continue to sequester CO{sub 2} for many years after the completion of the term of the DOE agreement. The objectives of this project are expected to be fulfilled by working through two distinct phases. The overall objective of Phase 1 was to develop a fully definitive project basis for a competitive Renewal Application process to proceed into Phase 2 - Design, Construction and Operations. Phase 1 includes the studies attached hereto that will establish: the engineering design basis for the capture, compression and transportation of CO{sub 2} from the LCC Gasification Project, and the criteria and specifications for a monitoring, verification and accounting (MVA) plan at the Hastings oil field in Texas. The overall objective of Phase 2, provided a successful competitive down-selection, is to execute design, construction and operations of three capital projects: (1) the CO{sub 2} capture and compression equipment, (2) a Connector Pipeline from the LLC Gasification Project to the Green Pipeline owned by Denbury and an affiliate of Denbury, and (3) a comprehensive MVA system at the Hastings oil field.« less

Angular Momentum Evolution of Young Stars in the nearby Scorpius-Centaurus OB Association

NASA Astrophysics Data System (ADS)

Mellon, Samuel N.; Mamajek, Eric E.; Oberst, Thomas E.; Pecaut, Mark J.

2017-07-01

We report the results of a study of archival SuperWASP light curves for stars in Scorpius-Centaurus (Sco-Cen), the nearest OB association. We use SuperWASP time-series photometry to extract rotation periods for 189 candidate members of the Sco-Cen complex and verify that 162 of those are members of the classic Sco-Cen subgroups of Upper Scorpius (US), Upper Centaurus-Lupus (UCL), and Lower Centaurus-Crux (LCC). This study provides the first measurements of rotation periods explicitly for large samples of pre-main-sequence (pre-MS) stars spanning the UCL and LCC subgroups. Our final sample of 157 well-characterized pre-MS stars spans ages of ˜10-20 Myr, spectral types of ˜F3-M0, and masses of M ≃ 0.3-1.5 {{ M }}⊙ {{N}}. For this sample, we find a distribution of stellar rotation periods with a median of P rot ≃ 2.4 days, an overall range of 0.2 < P rot < 8 days, and a fairly well-defined mass-dependent upper envelope of rotation periods. This distribution of periods is consistent with recently developed stellar angular momentum evolution models. These data are significant because they represent an undersampled age range and the number of measurable rotation periods is large compared to recent studies of other regions. We also search for new examples of eclipsing disk or ring systems analogous to 1SWASP J140747.93-394542.6 (J1407), but find none. Our survey yielded five eclipsing binaries, but only one appears to be physically associated with the Sco-Cen complex. V2394 Oph is a heavily reddened (A V ≃ 5 mag) massive contact binary in the LDN 1689 cloud whose Gaia astrometry is clearly consistent with kinematic membership with the Ophiuchus star-forming region.

National Soil Information System in Turkey

NASA Astrophysics Data System (ADS)

Emrah Erdogan, Hakki; Sahin, Mehmet; Sahin, Yuksel

2013-04-01

Land consolidation (LC) represents complexity if management, legal, economic and technical procedures realized in order to adjust the land structure according to actual human preferences and needs. It includes changes in ownership rights to land and other real estate property, exchange of parcels among owners, changes in parcel borders, parcel size and shape, joining and dividing of parcels, changes in land use, construction works as roads, bridges, water changes etc.. Since the subject of LC is agricultural lands, the quality of consolidation depends on the quality of soil data. General Directorate of Agrarian Reform (GDAR) is the responsible institution on land consolidation whole of Turkey. Under GDAR, National Soil Information System (NSIS) has been build up with base soil data in relevant scale (1:5000). NSIS contain detailed information on soil chemical and physical properties, current land use, parent material, land capability class, Storie Index Values. SI were used on land consolidation, land use planning and farm development services. LCC was used for land distribution, rental land; define of village settlement, consolidation, expropriation, reconstruction, reclamation, non-agricultural usage. LCC were also specified to subclasses in four different limited factors as i) flow and erosion risk ii) requirement of drainage and soil moisture iii) Limits of soil tillage and root (shallow soils, low water retention capacity, stony, salty .etc) iv) climatic limits. In this study, digital soil survey and mapping project located in Yumurtalik, Adana is presented as an example of NSIS data structure. The project cover an area of 45709 ha that include crop lands as an area of 28528 ha and other land use (urban, roads..etc) as an area of 17181 ha. Soil profiles were described in 45 different points and totally 1279 soil samples were collected in field study and the check bore hole were made in 3170 points.

Use of Treated Municipal Wastewater as Power Plant Cooling System Makeup Water: Tertiary Treatment versus Expanded Chemical Regimen for Recirculating Water Quality Management

DOE Office of Scientific and Technical Information (OSTI.GOV)

David Dzombak; Radisav Vidic; Amy Landis

Treated municipal wastewater is a common, widely available alternative source of cooling water for thermoelectric power plants across the U.S. However, the biodegradable organic matter, ammonia-nitrogen, carbonate and phosphates in the treated wastewater pose challenges with respect to enhanced biofouling, corrosion, and scaling, respectively. The overall objective of this study was to evaluate the benefits and life cycle costs of implementing tertiary treatment of secondary treated municipal wastewater prior to use in recirculating cooling systems. The study comprised bench- and pilot-scale experimental studies with three different tertiary treated municipal wastewaters, and life cycle costing and environmental analyses of various tertiarymore » treatment schemes. Sustainability factors and metrics for reuse of treated wastewater in power plant cooling systems were also evaluated. The three tertiary treated wastewaters studied were: secondary treated municipal wastewater subjected to acid addition for pH control (MWW_pH); secondary treated municipal wastewater subjected to nitrification and sand filtration (MWW_NF); and secondary treated municipal wastewater subjected nitrification, sand filtration, and GAC adsorption (MWW_NFG). Tertiary treatment was determined to be essential to achieve appropriate corrosion, scaling, and biofouling control for use of secondary treated municipal wastewater in power plant cooling systems. The ability to control scaling, in particular, was found to be significantly enhanced with tertiary treated wastewater compared to secondary treated wastewater. MWW_pH treated water (adjustment to pH 7.8) was effective in reducing scale formation, but increased corrosion and the amount of biocide required to achieve appropriate biofouling control. Corrosion could be adequately controlled with tolytriazole addition (4-5 ppm TTA), however, which was the case for all of the tertiary treated waters. For MWW_NF treated water, the removal of ammonia by nitrification helped to reduce the corrosivity and biocide demand. Also, the lower pH and alkalinity resulting from nitrification reduced the scaling to an acceptable level, without the addition of anti-scalant chemicals. Additional GAC adsorption treatment, MWW_NFG, yielded no net benefit. Removal of organic matter resulted in pitting corrosion in copper and cupronickel alloys. Negligible improvement was observed in scaling control and biofouling control. For all of the tertiary treatments, biofouling control was achievable, and most effectively with pre-formed monochloramine (2-3 ppm) in comparison with NaOCl and ClO2. Life cycle cost (LCC) analyses were performed for the tertiary treatment systems studied experimentally and for several other treatment options. A public domain conceptual costing tool (LC3 model) was developed for this purpose. MWW_SF (lime softening and sand filtration) and MWW_NF were the most cost-effective treatment options among the tertiary treatment alternatives considered because of the higher effluent quality with moderate infrastructure costs and the relatively low doses of conditioning chemicals required. Life cycle inventory (LCI) analysis along with integration of external costs of emissions with direct costs was performed to evaluate relative emissions to the environment and external costs associated with construction and operation of tertiary treatment alternatives. Integrated LCI and LCC analysis indicated that three-tiered treatment alternatives such as MWW_NSF and MWW_NFG, with regular chemical addition for treatment and conditioning and/or regeneration, tend to increase the impact costs and in turn the overall costs of tertiary treatment. River water supply and MWW_F alternatives with a single step of tertiary treatment were associated with lower impact costs, but the contribution of impact costs to overall annual costs was higher than all other treatment alternatives. MWW_NF and MWW_SF alternatives exhibited moderate external impact costs with moderate infrastructure and chemical conditioner dosing, which makes them (especially MWW_NF) better treatment alternatives from the environmental sustainability perspective since they exhibited minimal contribution to environmental damage from emissions.« less

David Grant Medical Center energy use baseline and integrated resource assessment

DOE Office of Scientific and Technical Information (OSTI.GOV)

Richman, E.E.; Hoshide, R.K.; Dittmer, A.L.

1993-04-01

The US Air Mobility Command (AMC) has tasked Pacific Northwest Laboratory (PNL) with supporting the US Department of Energy (DOE) Federal Energy Management Program`s (FEMP) mission to identify, evaluate, and assist in acquiring all cost-effective energy resource opportunities (EROs) at the David Grant Medical Center (DGMC). This report describes the methodology used to identify and evaluate the EROs at DGMC, provides a life-cycle cost (LCC) analysis for each ERO, and prioritizes any life-cycle cost-effective EROs based on their net present value (NPV), value index (VI), and savings to investment ratio (SIR or ROI). Analysis results are presented for 17 EROsmore » that involve energy use in the areas of lighting, fan and pump motors, boiler operation, infiltration, electric load peak reduction and cogeneration, electric rate structures, and natural gas supply. Typical current energy consumption is approximately 22,900 MWh of electricity (78,300 MBtu), 87,600 kcf of natural gas (90,300 MBtu), and 8,300 gal of fuel oil (1,200 MBtu). A summary of the savings potential by energy-use category of all independent cost-effective EROs is shown in a table. This table includes the first cost, yearly energy consumption savings, and NPV for each energy-use category. The net dollar savings and NPV values as derived by the life-cycle cost analysis are based on the 1992 federal discount rate of 4.6%. The implementation of all EROs could result in a yearly electricity savings of more than 6,000 MWh or 26% of current yearly electricity consumption. More than 15 MW of billable load (total billed by the utility for a 12-month period) or more than 34% of current billed demand could also be saved. Corresponding natural gas savings would be 1,050 kcf (just over 1% of current consumption). Total yearly net energy cost savings for all options would be greater than $343,340. This value does not include any operations and maintenance (O&M) savings.« less

David Grant Medical Center energy use baseline and integrated resource assessment

DOE Office of Scientific and Technical Information (OSTI.GOV)

Richman, E.E.; Hoshide, R.K.; Dittmer, A.L.

1993-04-01

The US Air Mobility Command (AMC) has tasked Pacific Northwest Laboratory (PNL) with supporting the US Department of Energy (DOE) Federal Energy Management Program's (FEMP) mission to identify, evaluate, and assist in acquiring all cost-effective energy resource opportunities (EROs) at the David Grant Medical Center (DGMC). This report describes the methodology used to identify and evaluate the EROs at DGMC, provides a life-cycle cost (LCC) analysis for each ERO, and prioritizes any life-cycle cost-effective EROs based on their net present value (NPV), value index (VI), and savings to investment ratio (SIR or ROI). Analysis results are presented for 17 EROsmore » that involve energy use in the areas of lighting, fan and pump motors, boiler operation, infiltration, electric load peak reduction and cogeneration, electric rate structures, and natural gas supply. Typical current energy consumption is approximately 22,900 MWh of electricity (78,300 MBtu), 87,600 kcf of natural gas (90,300 MBtu), and 8,300 gal of fuel oil (1,200 MBtu). A summary of the savings potential by energy-use category of all independent cost-effective EROs is shown in a table. This table includes the first cost, yearly energy consumption savings, and NPV for each energy-use category. The net dollar savings and NPV values as derived by the life-cycle cost analysis are based on the 1992 federal discount rate of 4.6%. The implementation of all EROs could result in a yearly electricity savings of more than 6,000 MWh or 26% of current yearly electricity consumption. More than 15 MW of billable load (total billed by the utility for a 12-month period) or more than 34% of current billed demand could also be saved. Corresponding natural gas savings would be 1,050 kcf (just over 1% of current consumption). Total yearly net energy cost savings for all options would be greater than $343,340. This value does not include any operations and maintenance (O M) savings.« less

Operations and support cost modeling of conceptual space vehicles

NASA Technical Reports Server (NTRS)

Ebeling, Charles

1994-01-01

The University of Dayton is pleased to submit this annual report to the National Aeronautics and Space Administration (NASA) Langley Research Center which documents the development of an operations and support (O&S) cost model as part of a larger life cycle cost (LCC) structure. It is intended for use during the conceptual design of new launch vehicles and spacecraft. This research is being conducted under NASA Research Grant NAG-1-1327. This research effort changes the focus from that of the first two years in which a reliability and maintainability model was developed to the initial development of an operations and support life cycle cost model. Cost categories were initially patterned after NASA's three axis work breakdown structure consisting of a configuration axis (vehicle), a function axis, and a cost axis. A revised cost element structure (CES), which is currently under study by NASA, was used to established the basic cost elements used in the model. While the focus of the effort was on operations and maintenance costs and other recurring costs, the computerized model allowed for other cost categories such as RDT&E and production costs to be addressed. Secondary tasks performed concurrent with the development of the costing model included support and upgrades to the reliability and maintainability (R&M) model. The primary result of the current research has been a methodology and a computer implementation of the methodology to provide for timely operations and support cost analysis during the conceptual design activities.

Automatic alignment of pre- and post-interventional liver CT images for assessment of radiofrequency ablation

NASA Astrophysics Data System (ADS)

Rieder, Christian; Wirtz, Stefan; Strehlow, Jan; Zidowitz, Stephan; Bruners, Philipp; Isfort, Peter; Mahnken, Andreas H.; Peitgen, Heinz-Otto

2012-02-01

Image-guided radiofrequency ablation (RFA) is becoming a standard procedure for minimally invasive tumor treatment in clinical practice. To verify the treatment success of the therapy, reliable post-interventional assessment of the ablation zone (coagulation) is essential. Typically, pre- and post-interventional CT images have to be aligned to compare the shape, size, and position of tumor and coagulation zone. In this work, we present an automatic workflow for masking liver tissue, enabling a rigid registration algorithm to perform at least as accurate as experienced medical experts. To minimize the effect of global liver deformations, the registration is computed in a local region of interest around the pre-interventional lesion and post-interventional coagulation necrosis. A registration mask excluding lesions and neighboring organs is calculated to prevent the registration algorithm from matching both lesion shapes instead of the surrounding liver anatomy. As an initial registration step, the centers of gravity from both lesions are aligned automatically. The subsequent rigid registration method is based on the Local Cross Correlation (LCC) similarity measure and Newton-type optimization. To assess the accuracy of our method, 41 RFA cases are registered and compared with the manually aligned cases from four medical experts. Furthermore, the registration results are compared with ground truth transformations based on averaged anatomical landmark pairs. In the evaluation, we show that our method allows to automatic alignment of the data sets with equal accuracy as medical experts, but requiring significancy less time consumption and variability.

Vehicle Assembly Building Fire Mishap Investigation Report. Volume I of V

NASA Technical Reports Server (NTRS)

Kight, Ira; Luciano, Steven; Stevens, Michael B.; Farley, W. Max; Collins, Bryce D.; Potterger, William C.; Levesque, Jodi

2005-01-01

On January 13, 2005, at approximately 1355, smoke was noticed on the 4th floor of D Tower in the Vehicle Assembly Building (VAB). Subsequently, a 911 call was made, a fire alarm pull station was activated, and the VAB was evacuated. The source of the smoke was determined to be a fire on the Low Bay M/N section roof near the Launch Control Center (LCC) Crossover. Due to the high visibility of the mishap, the KSC Center Director appointed a Mishap Investigation Board. Damage to government property was limited to the roof and a small number of ceiling tiles that were damaged by the fire fighters during the response. At the time of the mishap, there were hazardous commodities in the VAB including Solid Rocket Motors (SRMs) with open grain due to Solid Rocket Booster (SRB) igniter inspections. The Board agrees with the SGS Fire Services' theory that large amounts of smoke concentrated in the VAB D Tower and moved downward into the cable tunnel. The Board determined the proximate cause of this incident to be torching. HRI was installing a torch applied roof membrane which resulted in the ignition of combustible materials under the membrane near a wooden roof expansion joint. The torch applied roofing method is a universally accepted safe industry practice when applied to non-combustible surfaces. The combination of an open flame torch and combustible materiaLs presents an increased level of risk even with skilled applicators. The addition of high winds to this combination results in a risk the Board thinks can not be adequately mitigated. An appropriate risk assessment and analysis must be performed on the proposed roofing method to be used on high visibility facilities which represent unique national assets even when using common industry practices for repair and modification. The Board identified three root causes which contributed to or created the proximate cause and, if eliminated or modified, would have prevented the mishap: 1. Combustible materials in existing roof system 2. Wind speed and direction 3. Inadequate fire watch technique. Two contributing factors were identified which may have contributed to the occurrence but, if eliminated or modified, would not have prevented the occurrence: 1. HRI rushed to dry in and seal the roof on January 13 because heavy rain was predicted for the next day 2. No guidance on torching in windy conditions A total of 17 significant observations were noted during this investigation, which could lead to another mishap, or increase the severity of a mishap, but were not contributing factors in this mishap.

Application of Design of Experiments and Surrogate Modeling within the NASA Advanced Concepts Office, Earth-to-Orbit Design Process

NASA Technical Reports Server (NTRS)

Zwack, Mathew R.; Dees, Patrick D.; Holt, James B.

2016-01-01

Decisions made during early conceptual design have a large impact upon the expected life-cycle cost (LCC) of a new program. It is widely accepted that up to 80% of such cost is committed during these early design phases. Therefore, to help minimize LCC, decisions made during conceptual design must be based upon as much information as possible. To aid in the decision making for new launch vehicle programs, the Advanced Concepts Office (ACO) at NASA Marshall Space Flight Center (MSFC) provides rapid turnaround pre-phase A and phase A concept definition studies. The ACO team utilizes a proven set of tools to provide customers with a full vehicle mass breakdown to tertiary subsystems, preliminary structural sizing based upon worst-case flight loads, and trajectory optimization to quantify integrated vehicle performance for a given mission. Although the team provides rapid turnaround for single vehicle concepts, the scope of the trade space can be limited due to analyst availability and the manpower requirements for manual execution of the analysis tools. In order to enable exploration of a broader design space, the ACO team has implemented an advanced design methods (ADM) based approach. This approach applies the concepts of design of experiments (DOE) and surrogate modeling to more exhaustively explore the trade space and provide the customer with additional design information to inform decision making. This paper will first discuss the automation of the ACO tool set, which represents a majority of the development effort. In order to fit a surrogate model within tolerable error bounds a number of DOE cases are needed. This number will scale with the number of variable parameters desired and the complexity of the system's response to those variables. For all but the smallest design spaces, the number of cases required cannot be produced within an acceptable timeframe using a manual process. Therefore, automation of the tools was a key enabler for the successful application of an ADM approach to an ACO design study. Following the overview of the tool set automation, an example problem will be given to illustrate the implementation of the ADM approach. The example problem will first cover the inclusion of ground rules and assumptions (GR&A) for a study. The GR&A are very important to the study as they determine the constraints within which a trade study can be conducted. These trades must ultimately reconcile with the customer's desired output and any anticipated "what if" questions. The example problem will then illustrate the setup and execution of a DOE through the automated ACO tools. This process is accomplished more efficiently in this work by splitting the tools into two separate environments. The first environment encompasses the structural optimization and mass estimation tools, while the second is focused on trajectory optimization. Surrogate models are fit to the outputs of each environment and are "integrated" via connection of the surrogate equations. Throughout this process, checks are implemented to compare the output of the surrogates to the output of manually run cases to ensure that the error of the final surrogates is at an acceptable level. The conclusion of the example problem demonstrates the utility of the ADM based approach. Using surrogate models gives the ACO team the ability to visualize vehicle sensitivities to various design parameters and identify regions of interest within the design space. The ADM approach can thus be used to inform concept down selection and isolate promising vehicle configurations to be explored in more detail through the manual design process. In addition it provides the customer with an almost instantaneous turnaround on any ''what if" questions that may arise within the bounds of the surrogate model. This approach ultimately expands the ability of the ACO team to provide its customer with broad and rapid turnaround trade studies for launch vehicle conceptual design. The ability to identify a selection of designs which can meet the customer requirements will help ensure lower LCC of launch vehicle designs originating from ACO.

Application of Design of Experiments and Surrogate Modeling within the NASA Advanced Concepts Office, Earth-to-Orbit Design Process

NASA Technical Reports Server (NTRS)

Zwack, Mathew R.; Dees, Patrick D.; Holt, James B.

2016-01-01

Decisions made during early conceptual design have a large impact upon the expected life-cycle cost (LCC) of a new program. It is widely accepted that up to 80% of such cost is committed during these early design phases.1 Therefore, to help minimize LCC, decisions made during conceptual design must be based upon as much information as possible. To aid in the decision making for new launch vehicle programs, the Advanced Concepts Office (ACO) at NASA Marshall Space Flight Center (MSFC) provides rapid turnaround pre-phase A and phase A concept definition studies. The ACO team utilizes a proven set of tools to provide customers with a full vehicle mass breakdown to tertiary subsystems, preliminary structural sizing based upon worst-case flight loads, and trajectory optimization to quantify integrated vehicle performance for a given mission.2 Although the team provides rapid turnaround for single vehicle concepts, the scope of the trade space can be limited due to analyst availability and the manpower requirements for manual execution of the analysis tools. In order to enable exploration of a broader design space, the ACO team has implemented an Advanced Design Methods (ADM) based approach. This approach applies the concepts of Design of Experiments (DOE) and surrogate modeling to more exhaustively explore the trade space and provide the customer with additional design information to inform decision making. This paper will first discuss the automation of the ACO tool set, which represents a majority of the development e ort. In order to t a surrogate model within tolerable error bounds a number of DOE cases are needed. This number will scale with the number of variable parameters desired and the complexity of the system's response to those variables. For all but the smallest design spaces, the number of cases required cannot be produced within an acceptable timeframe using a manual process. Therefore, automation of the tools was a key enabler for the successful application of an ADM approach to an ACO design study. Following the overview of the tool set automation, an example problem will be given to illustrate the implementation of the ADM approach. The example problem will first cover the inclusion of Ground Rules and Assumptions (GR&A) for a study. The GR&A are very important to the study as they determine the constraints within which a trade study can be conducted. These trades must ultimately reconcile with the customer's desired output and any anticipated \\what if" questions. The example problem will then illustrate the setup and execution of a DOE through the automated ACO tools. This process is accomplished more efficiently in this work by splitting the tools into two separate environments. The first environment encompasses the structural optimization and mass estimation tools, while the second is focused on trajectory optimization. Surrogate models are t to the outputs of each environment and are integrated via connection of the surrogate equations. Throughout this process, checks are implemented to compare the output of the surrogates to the output of manually run cases to ensure that the error of the final surrogates is at an acceptable level. The conclusion of the example problem demonstrates the utility of the ADM based approach. Using surrogate models gives the ACO team the ability to visualize vehicle sensitivities to various design parameters and identify regions of interest within the design space. The ADM approach can thus be used to inform concept down selection and isolate promising vehicle configurations to be explored in more detail through the manual design process. In addition it provides the customer with an almost instantaneous turnaround on any \\what if" questions that may arise within the bounds of the surrogate model. This approach ultimately expands the ability of the ACO team to provide its customer with broad and rapid turnaround trade studies for launch vehicle conceptual design. The ability to identify a selection of designs which can meet the customer requirements will have the potential to lower LCC of launch vehicle designs originating from ACO.

2ND Annual Report on Contract N00014-88-K-0059

DTIC Science & Technology

1989-10-01

dogs, and adrenally insufficient humans are very fragile and do not survive this type of stress. Further studies in this lab have revealed that the...epinephrine and dopamine . Life Sci 21:625. 33) Pirkle JC, Gann DS, Allen-Rolands CF 1982 role of the pituitary in restitution of blood volume after...represent means+ SE. 17 Li z LO 0~0 0 I0 0 0r -C C a- C 0 00 00 Li LCC) 0 1 (lw/6d) HIDV Uol G6uDJ) Plasma AVP (pg/ml) 10 co C Do-+- 3 CA / 0j x N~ Q~N + 0

Optimization strategies with resource scarcity: From immunization of networks to the traveling salesman problem

NASA Astrophysics Data System (ADS)

Bellingeri, Michele; Agliari, Elena; Cassi, Davide

2015-10-01

The best strategy to immunize a complex network is usually evaluated in terms of the percolation threshold, i.e. the number of vaccine doses which make the largest connected cluster (LCC) vanish. The strategy inducing the minimum percolation threshold represents the optimal way to immunize the network. Here we show that the efficacy of the immunization strategies can change during the immunization process. This means that, if the number of doses is limited, the best strategy is not necessarily the one leading to the smallest percolation threshold. This outcome should warn about the adoption of global measures in order to evaluate the best immunization strategy.

Thermal Testing and Quality Assurance of BGA LCC & QFN Electronic Packages

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kuper, Cameron Mathias

The purpose of this project is to experimentally validate the thermal fatigue life of solder interconnects for a variety of surface mount electronic packages. Over the years, there has been a significant amount of research and analysis in the fracture of solder joints on printed circuit boards. Solder is important in the mechanical and electronic functionality of the component. It is important throughout the life of the product that the solder remains crack and fracture free. The specific type of solder used in this experiment is a 63Sn37Pb eutectic alloy. Each package was surrounded conformal coating or underfill material.

Design and operations technologies - Integrating the pieces. [for future space systems design

NASA Technical Reports Server (NTRS)

Eldred, C. H.

1979-01-01

As major elements of life-cycle costs (LCC) having critical impacts on the initiation and utilization of future space programs, the areas of vehicle design and operations are reviewed in order to identify technology requirements. Common to both areas is the requirement for efficient integration of broad, complex systems. Operations technologies focus on the extension of space-based capabilities and cost reduction through the combination of innovative design, low-maintenance hardware, and increased manpower productivity. Design technologies focus on computer-aided techniques which increase productivity while maintaining a high degree of flexibility which enhances creativity and permits graceful design changes.

An Analysis of Peak Wind Speed Data from Collocated Mechanical and Ultrasonic Anemometers

NASA Technical Reports Server (NTRS)

Short, David A.; Wells, Leonard; Merceret, Francis J.; Roeder, William P.

2007-01-01

This study compared peak wind speeds reported by mechanical and ultrasonic anemometers at Cape Canaveral Air Force Station and Kennedy Space Center (CCAFS/KSC) on the east central coast of Florida and Vandenberg Air Force Base (VAFB) on the central coast of California. Launch Weather Officers, forecasters, and Range Safety analysts need to understand the performance of wind sensors at CCAFS/KSC and VAFB for weather warnings, watches, advisories, special ground processing operations, launch pad exposure forecasts, user Launch Commit Criteria (LCC) forecasts and evaluations, and toxic dispersion support. The legacy CCAFS/KSC and VAFB weather tower wind instruments are being changed from propeller-and-vane (CCAFS/KSC) and cup-and-vane (VAFB) sensors to ultrasonic sensors under the Range Standardization and Automation (RSA) program. Mechanical and ultrasonic wind measuring techniques are known to cause differences in the statistics of peak wind speed as shown in previous studies. The 45th Weather Squadron (45 WS) and the 30th Weather Squadron (30 WS) requested the Applied Meteorology Unit (AMU) to compare data between the RSA ultrasonic and legacy mechanical sensors to determine if there are significant differences. Note that the instruments were sited outdoors under naturally varying conditions and that this comparison was not designed to verify either technology. Approximately 3 weeks of mechanical and ultrasonic wind data from each range from May and June 2005 were used in this study. The CCAFS/KSC data spanned the full diurnal cycle, while the VAFB data were confined to 1000-1600 local time. The sample of 1-minute data from numerous levels on five different towers on each range totaled more than 500,000 minutes of data (482,979 minutes of data after quality control). The ten towers were instrumented at several levels, ranging from 12 ft to 492 ft above ground level. The ultrasonic sensors were collocated at the same vertical levels as the mechanical sensors and typically within 15 ft horizontally of each another. Data from a total of 53 RSA ultrasonic sensors, collocated with mechanical sensors were compared. The 1- minute average wind speed/direction and the 1-second peak wind speed/direction were compared.

U.S. Department of the Interior Southeast Climate Science Center Science and Operational Plan

USGS Publications Warehouse

Jones, Sonya A.; Dalton, Melinda S.

2012-01-01

Climate change challenges many of the basic assumptions routinely used by conservation planners and managers, including the identification and prioritization of areas for conservation based on current environmental conditions and the assumption those conditions could be controlled by management actions. Climate change will likely alter important ecosystem drivers (temperature, precipitation, and sea-level rise) and make it difficult, if not impossible, to maintain current environmental conditions into the future. Additionally, the potential for future conservation of non-conservation lands may be affected by climate change, which further complicates resource planning. Potential changes to ecosystem drivers, as a result of climate change, highlight the need to develop and adapt effective conservation strategies to cope with the effects of climate and landscape change. The U.S. Congress, recognized the potential effects of climate change and authorized the creation of the U.S. Geological Survey National Climate Change and Wildlife Science Center (NCCWSC) in 2008. The directive of the NCCWSC is to produce science that supports resource-management agencies as they anticipate and adapt to the effects of climate change on fish, wildlife, and their habitats. On September 14, 2009, U.S. Department of the Interior (DOI) Secretary Ken Salazar signed Secretarial Order 3289 (amended February 22, 2010), which expanded the mandate of the NCCWSC to address climate-change-related impacts on all DOI resources. Secretarial Order 3289 "Addressing the Impacts of Climate Change on America's Water, Land, and Other Natural and Cultural Resources," established the foundation of two partner-based conservation science entities: Climate Science Centers (CSC) and their primary partners, Landscape Conservation Cooperatives (LCC). CSCs and LCCs are the Department-wide approach for applying scientific tools to increase the understanding of climate change, and to coordinate an effective response to its impacts on tribes and the land, water, ocean, fish and wildlife, and cultural-heritage resources that DOI manages. The NCCWSC is establishing a network of eight DOI CSCs (Alaska, Southeast, Northwest, North Central, Pacific Islands, Southwest, Northeast, and South Central) that will work with a variety of partners and stakeholders to provide resource managers the tools and information they need to help them anticipate and adapt conservation planning and design for projected climate change. The Southeast CSC, a federally led research collaboration hosted by North Carolina State University, was established in 2010. The Southeast CSC brings together the expertise of federal and university scientists to address climate-change priority needs of federal, state, non-governmental, and tribal resource managers. This document is the first draft of a science and operational plan for the Southeast CSC. The document describes operational considerations, provides the context for climate-change impacts in the Southeastern United States, and establishes six major science themes the Southeast CSC will address in collaboration with partners. This document is intended to be reevaluated and modified as partner needs change.

Methodology for Evaluating Cost-effectiveness of Commercial Energy Code Changes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hart, Philip R.; Liu, Bing

This document lays out the U.S. Department of Energy’s (DOE’s) method for evaluating the cost-effectiveness of energy code proposals and editions. The evaluation is applied to provisions or editions of the American Society of Heating, Refrigerating and Air-Conditioning Engineers (ASHRAE) Standard 90.1 and the International Energy Conservation Code (IECC). The method follows standard life-cycle cost (LCC) economic analysis procedures. Cost-effectiveness evaluation requires three steps: 1) evaluating the energy and energy cost savings of code changes, 2) evaluating the incremental and replacement costs related to the changes, and 3) determining the cost-effectiveness of energy code changes based on those costs andmore » savings over time.« less

A Survey of Human Factors Methodologies and Models for Improving the Maintainability Design of Emerging Army Aviation Systems

DTIC Science & Technology

1990-02-01

V)*g 3, 44 4H 44 W M. s- 0 M 0) >t W 04.)0 It lU 44G M 1 >1 4 4 >4C -H> EU ) A d Ix 44to I H ,4 u- j . -H 0 0 9 L$cC4c 01 0 HO a)0 E-1 14 -4 -0 EUH )U 0...materiel maintenance policy and retail maintenance operations (AR 750-1). Washington, DC: Headquarters, Department of the Army. Drury, D . G., Paramore , B...Associates. Folley, J . D ., Jr., & Altman, J . W. (1956). Guide to design of electronic equipment for maintainability (Report WADC-TR-56- 218). Wright

Beach Profile Analysis System (BPAS). Volume VIII. Supporting Appendixes for BPAS User’s Guides.

DTIC Science & Technology

1982-06-01

V) 0 4)C CC0~ID ~C ~ C C~ a) L C C~ ~ C L- d C C C a . C.. -’ -C--C 0 ) w 0 0 (a) 0 (L 00.0V a) L)~a LCC Uda C - -La r CC-->C> C ) a ).C C...2+"- 21 14 1 4 1 a7Sooo2 IIoQ2’l 4 0# 1la+ t0+ 1ab # 37# 153s So* 185# .8. l8i 340 3 w 2 4 7+ 1# 1 + l o+t 174+ 1IS+ 100+ lab+ 147’ + 1 + 1 9Q a IIIa 1

Using Technology Readiness Level (TRL), Life Cycle Cost (LCC), and Other Metrics to Supplement Equivalent System Mass (ESM) in Advanced Life Support (ALS)

NASA Technical Reports Server (NTRS)

Jones, Harry

2003-01-01

The ALS project plan goals are reducing cost, improving performance, and achieving flight readiness. ALS selects projects to advance the mission readiness of low cost, high performance technologies. The role of metrics is to help select good projects and report progress. The Equivalent Mass (EM) of a system is the sum of the estimated mass of the hardware, of its required materials and spares, and of the pressurized volume, power supply, and cooling system needed to support the hardware in space. EM is the total payload launch mass needed to provide and support a system. EM is directly proportional to the launch cost.

Characteristics of W-26% Re Target Material(LCC-0103)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sunwoo, A.

2003-10-07

The W-26 wt-% Re alloy was selected as a Stanford Linear Collider (SLC) target material for its exceptional physics properties and for the high strength and good ductility at the anticipated target operating temperatures, above the DBTT. After several years of operation, the target failed catastrophically. A detailed microstructural and mechanical characterization of the non-irradiated disk indicates that the material has been PM processed, nonuniformly mechanically worked and stress relieved. As a result, the ductility of the material varies through the thickness of the disk, making it difficult to determine the DBTT. The results of tensile and fatigue properties aremore » reported with the corresponding fractography of the fracture surfaces.« less

Model Development of Cold Chains for Fresh Fruits and Vegetables Distribution: A Case Study in Bali Province

NASA Astrophysics Data System (ADS)

Waisnawa, I. N. G. S.; Santosa, I. D. M. C.; Sunu, I. P. W.; Wirajati, IGAB

2018-01-01

In developing countries such as Indonesia, as much as 40% of total vegetables and fruits production becomes waste because of lack refrigeration. This condition also contributes a food crisis problem besides other factor such as, climate change and number of population. Cold chain system that will be modelled in this study is for vegetables and fruits and refrigeration system as the main devices. In future, this system will play an important role for the food crisis solution where fresh food can be distributed very well with significant low waste. The fresh food also can be kept with good quality and hygienist (bacteria contaminated). Cold Chain model will be designed using refrigeration components including, pre cooling chiller, cold room, and truck refrigeration. This study will be conducted by survey and observation di around Bali Province focus on vegetables and fruits production center. Interviews and questionnaire will be also done to get some information about the conventional distribution obstacles and problem. Distribution mapping will be developed and created. The data base of the storage characteristic of the fruits and vegetable also collected through experiment and secondary data. Depend on the mapping and data base can be developed a cold chain model that has the best performance application. The model will be can directly apply in Bali to get eligible cold chain in Bali. The cold chain model will be compared with the conventional distribution system using ALCC/LCC method and also others factor and will be weighted to get better results.

Computational analysis of the regulation of Ca2+ dynamics in rat ventricular myocytes

NASA Astrophysics Data System (ADS)

Bugenhagen, Scott M.; Beard, Daniel A.

2015-10-01

Force-frequency relationships of isolated cardiac myocytes show complex behaviors that are thought to be specific to both the species and the conditions associated with the experimental preparation. Ca2+ signaling plays an important role in shaping the force-frequency relationship, and understanding the properties of the force-frequency relationship in vivo requires an understanding of Ca2+ dynamics under physiologically relevant conditions. Ca2+ signaling is itself a complicated process that is best understood on a quantitative level via biophysically based computational simulation. Although a large number of models are available in the literature, the models are often a conglomeration of components parameterized to data of incompatible species and/or experimental conditions. In addition, few models account for modulation of Ca2+ dynamics via β-adrenergic and calmodulin-dependent protein kinase II (CaMKII) signaling pathways even though they are hypothesized to play an important regulatory role in vivo. Both protein-kinase-A and CaMKII are known to phosphorylate a variety of targets known to be involved in Ca2+ signaling, but the effects of these pathways on the frequency- and inotrope-dependence of Ca2+ dynamics are not currently well understood. In order to better understand Ca2+ dynamics under physiological conditions relevant to rat, a previous computational model is adapted and re-parameterized to a self-consistent dataset obtained under physiological temperature and pacing frequency and updated to include β-adrenergic and CaMKII regulatory pathways. The necessity of specific effector mechanisms of these pathways in capturing inotrope- and frequency-dependence of the data is tested by attempting to fit the data while including and/or excluding those effector components. We find that: (1) β-adrenergic-mediated phosphorylation of the L-type calcium channel (LCC) (and not of phospholamban (PLB)) is sufficient to explain the inotrope-dependence; and (2) that CaMKII-mediated regulation of neither the LCC nor of PLB is required to explain the frequency-dependence of the data.

Validating the use of a luciferase labeled breast cancer cell line, MDA435LCC6, as a means to monitor tumor progression and to assess the therapeutic activity of an established anticancer drug, docetaxel (Dt) alone or in combination with the ILK inhibitor, QLT0267

PubMed Central

Anantha, Malathi; Warburton, Corinna; Waterhouse, Dawn; Yan, Hong; Yang, Young-joo; Strut, Dita; Osooly, Maryam; Masin, Dana; Bally, Marcel B

2011-01-01

A significant issue in drug efficacy studies is animal study design. Here we hypothesize that when evaluating new or existing therapeutics for the treatment of cancer, the location of disease burden will influence drug efficacy. To study this, female NCr nude mice were inoculated with luciferase-positive human breast cancer cells (LCC6WT-luc) orthotopically (o.t.), intraperitoneally (i.p.) or intracardiacly (i.c.) to create localized, ascites or disseminated disease, respectively. Tumor development was monitored using bioluminescence imaging. Docetaxel (Dt) pharmacokinetics and distribution to sites of tumor growth were determined. Disease progression was followed in animals treated with Dt alone and in combination with QLT0267, an integrin linked kinase inhibitor. Tumor related morbidity was most rapid when cells were inoculated i.c., where disease progression was observed in brain, ovaries, adrenal glands and lungs. Dt pharmacokinetics were comparable regardless of the model used (mean plasma AUC0–24 hrs 482.6 ng/ml*hr), however, Dt levels were lowest in those tissues developing disease following i.c. cell injection. Treatment with low dose Dt (5 mg/kg) increased overall survival and reduced tumor cell growth in all three models but the activity was greatest in mice with orthotopic tumors. Higher doses of Dt (15 mg/kg) was able to prolong survival in animals bearing i.p. tumors but not i.c. tumors. Addition of QLT0267 provided no added benefit above Dt alone in the disseminated model. These studies highlight a need for more comprehensive in vivo efficacy studies designed to assess multiple disease models and multiple endpoints, focusing analysis of drug parameters on the most chemoresistant disease. PMID:21358264

Knockdown of estrogen receptor-α induces autophagy and inhibits antiestrogen-mediated unfolded protein response activation, promoting ROS-induced breast cancer cell death

PubMed Central

Cook, Katherine L.; Clarke, Pamela A. G.; Parmar, Jignesh; Hu, Rong; Schwartz-Roberts, Jessica L.; Abu-Asab, Mones; Wärri, Anni; Baumann, William T.; Clarke, Robert

2014-01-01

Approximately 70% of all newly diagnosed breast cancers express estrogen receptor (ER)-α. Although inhibiting ER action using targeted therapies such as fulvestrant (ICI) is often effective, later emergence of antiestrogen resistance limits clinical use. We used antiestrogen-sensitive and -resistant cells to determine the effect of antiestrogens/ERα on regulating autophagy and unfolded protein response (UPR) signaling. Knockdown of ERα significantly increased the sensitivity of LCC1 cells (sensitive) and also resensitized LCC9 cells (resistant) to antiestrogen drugs. Interestingly, ERα knockdown, but not ICI, reduced nuclear factor (erythroid-derived 2)-like (NRF)-2 (UPR-induced antioxidant protein) and increased cytosolic kelch-like ECH-associated protein (KEAP)-1 (NRF2 inhibitor), consistent with the observed increase in ROS production. Furthermore, autophagy induction by antiestrogens was prosurvival but did not prevent ERα knockdown–mediated death. We built a novel mathematical model to elucidate the interactions among UPR, autophagy, ER signaling, and ROS regulation of breast cancer cell survival. The experimentally validated mathematical model explains the counterintuitive result that knocking down the main target of ICI (ERα) increased the effectiveness of ICI. Specifically, the model indicated that ERα is no longer present in excess and that the effect on proliferation from further reductions in its level by ICI cannot be compensated for by increased autophagy. The stimulation of signaling that can confer resistance suggests that combining autophagy or UPR inhibitors with antiestrogens would reduce the development of resistance in some breast cancers.—Cook, K. L., Clarke, P. A. G., Parmar, J., Hu, R., Schwartz-Roberts, J. L., Abu-Asab, M., Wärri, A., Baumann, W. T., Clarke, R. Knockdown of estrogen receptor-α induces autophagy and inhibits antiestrogen-mediated unfolded protein response activation, promoting ROS-induced breast cancer cell death. PMID:24858277

Trait-abundance relation in response to nutrient addition in a Tibetan alpine meadow: The importance of species trade-off in resource conservation and acquisition.

PubMed

Liu, Huiying; Li, Ying; Ren, Fei; Lin, Li; Zhu, Wenyan; He, Jin-Sheng; Niu, Kechang

2017-12-01

In competition-dominated communities, traits promoting resource conservation and competitive ability are expected to have an important influence on species relative abundance (SRA). Yet, few studies have tested the trait-abundance relations in the line of species trade-off in resource conservation versus acquisition, indicating by multiple traits coordination. We measured SRA and key functional traits involving leaf economic spectrum (SLA, specific leaf area; LDMC, leaf dry matter content; LCC, leaf carbon concentration; LNC, leaf nitrogen concentration; LPC, leaf phosphorus concentration; Hs, mature height) for ten common species in all plots subjected to addition of nitrogen fertilizer (N), phosphorus fertilizer (P), or both of them (NP) in a Tibetan alpine meadow. We test whether SRA is positively related with traits promoting plant resource conservation, while negatively correlated with traits promoting plant growth and resource acquisition. We found that species were primarily differentiated along a trade-off axis involving traits promoting nutrient acquisition and fast growth (e.g., LPC and SLA) versus traits promoting resource conservation and competition ability (e.g., large LDMC). We further found that SRA was positively correlated with plant height, LDMC, and LCC, but negatively associated with SLA and leaf nutrient concentration irrespective of fertilization. A stronger positive height-SRA was found in NP-fertilized plots than in other plots, while negative correlations between SRA and SLA and LPC were found in N or P fertilized plots. The results indicate that species trade-off in nutrient acquisition and resource conservation was a key driver of SRA in competition-dominated communities following fertilization, with the linkage between SRA and traits depending on plant competition for specific soil nutrient and/or light availability. The results highlight the importance of competitive exclusion in plant community assembly following fertilization and suggest that abundant species in local communities become dominated at expense of growth while infrequent species hold an advantage in fast growth and dispersals to neighbor meta-communities.

Using System Mass (SM), Equivalent Mass (EM), Equivalent System Mass (ESM) or Life Cycle Mass (LCM) in Advanced Life Support (ALS) Reporting

NASA Technical Reports Server (NTRS)

Jones, Harry

2003-01-01

The Advanced Life Support (ALS) has used a single number, Equivalent System Mass (ESM), for both reporting progress and technology selection. ESM is the launch mass required to provide a space system. ESM indicates launch cost. ESM alone is inadequate for technology selection, which should include other metrics such as Technology Readiness Level (TRL) and Life Cycle Cost (LCC) and also consider perfom.arxe 2nd risk. ESM has proven difficult to implement as a reporting metric, partly because it includes non-mass technology selection factors. Since it will not be used exclusively for technology selection, a new reporting metric can be made easier to compute and explain. Systems design trades-off performance, cost, and risk, but a risk weighted cost/benefit metric would be too complex to report. Since life support has fixed requirements, different systems usually have roughly equal performance. Risk is important since failure can harm the crew, but it is difficult to treat simply. Cost is not easy to estimate, but preliminary space system cost estimates are usually based on mass, which is better estimated than cost. Amass-based cost estimate, similar to ESM, would be a good single reporting metric. The paper defines and compares four mass-based cost estimates, Equivalent Mass (EM), Equivalent System Mass (ESM), Life Cycle Mass (LCM), and System Mass (SM). EM is traditional in life support and includes mass, volume, power, cooling and logistics. ESM is the specifically defined ALS metric, which adds crew time and possibly other cost factors to EM. LCM is a new metric, a mass-based estimate of LCC measured in mass units. SM includes only the factors of EM that are originally measured in mass, the hardware and logistics mass. All four mass-based metrics usually give similar comparisons. SM is by far the simplest to compute and easiest to explain.