[ANTIMICROBIAL ACTION OF NOCARDIA VACCINII IMV B-7405 SURFACTANTS].

PubMed

Pirog, T P; Beregova, K A; Savenko, I V; Shevchuk, T A; Iutynska, G O

2015-01-01

To study the effect of Nocardia vaccinii IMV B-7405 surfactants on some bacteria (including pathogens of genera Proteus, Staphylococcus, Enterobacter), yeast of Candida species and fungi (Aspergillus niger R-3, Fusarium culmorum T-7). The antimi- crobial properties of surfactant were determined in suspension culture by Koch method and also by index of the minimum inhibitory concentration. Surfactants were extracted from supernatant of cultural liquid by mixture of chloroform and methanol (2:1). It is shown that the antimicrobial properties of N. vaccinii IMV B-7405 surfactant depended on the degree of purification (supernatant, solution of surfactant), concentration and exposure. Survival of Escherichia coli IEM-1 and Bacillus subtilis BT-2 (both vegetative cells and spores) after treatment for 1-2 hours with surfactants solution and the supernatant (the surfactant concentration 21 µg/ml) was 3-28%. Minimum inhibitory concentrations of N. vaccinii IMV B-7405 surfactants on studied bacteria, yeast and micromycetes were 11.5-85.0; 11.5-22.5 and 165.0-325.0 µ/ml respectively. Minimum inhibitory concentrations of N. vaccinii IMV B-7405 surfactants are comparable to those of the known microbial surfactants. The possibility of using the supernatant of culture liquid as an effective antimicrobial agent noticeably simplifies and reduces the cost of the technology of its obtaining.

30 CFR 740.5 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-07-01

... including, with respect to leased Federal coal, the Mineral Leasing Act and its implementing regulations... PROGRAM GENERAL REQUIREMENTS FOR SURFACE COAL MINING AND RECLAMATION OPERATIONS ON FEDERAL LANDS § 740.5... official action on behalf of a Federal agency that has administrative jurisdiction over Federal lands. Coal...

30 CFR 740.5 - Definitions.

Code of Federal Regulations, 2011 CFR

2011-07-01

... including, with respect to leased Federal coal, the Mineral Leasing Act and its implementing regulations... PROGRAM GENERAL REQUIREMENTS FOR SURFACE COAL MINING AND RECLAMATION OPERATIONS ON FEDERAL LANDS § 740.5... official action on behalf of a Federal agency that has administrative jurisdiction over Federal lands. Coal...

30 CFR 740.5 - Definitions.

Code of Federal Regulations, 2012 CFR

2012-07-01

... including, with respect to leased Federal coal, the Mineral Leasing Act and its implementing regulations... PROGRAM GENERAL REQUIREMENTS FOR SURFACE COAL MINING AND RECLAMATION OPERATIONS ON FEDERAL LANDS § 740.5... official action on behalf of a Federal agency that has administrative jurisdiction over Federal lands. Coal...

30 CFR 740.5 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-07-01

... including, with respect to leased Federal coal, the Mineral Leasing Act and its implementing regulations... PROGRAM GENERAL REQUIREMENTS FOR SURFACE COAL MINING AND RECLAMATION OPERATIONS ON FEDERAL LANDS § 740.5... official action on behalf of a Federal agency that has administrative jurisdiction over Federal lands. Coal...

40 CFR 62.7405 - Identification of plan-negative declaration.

Code of Federal Regulations, 2012 CFR

2012-07-01

... PROGRAMS (CONTINUED) APPROVAL AND PROMULGATION OF STATE PLANS FOR DESIGNATED FACILITIES AND POLLUTANTS New Hampshire Emissions from Existing Municipal Solid Waste Landfills § 62.7405 Identification of plan-negative declaration. On July 22, 1998, the New Hampshire Department of Environmental Services submitted a letter...

40 CFR 62.7405 - Identification of plan-negative declaration.

Code of Federal Regulations, 2014 CFR

2014-07-01

... PROGRAMS (CONTINUED) APPROVAL AND PROMULGATION OF STATE PLANS FOR DESIGNATED FACILITIES AND POLLUTANTS New Hampshire Emissions from Existing Municipal Solid Waste Landfills § 62.7405 Identification of plan-negative declaration. On July 22, 1998, the New Hampshire Department of Environmental Services submitted a letter...

40 CFR 62.7405 - Identification of plan-negative declaration.

Code of Federal Regulations, 2011 CFR

2011-07-01

... PROGRAMS (CONTINUED) APPROVAL AND PROMULGATION OF STATE PLANS FOR DESIGNATED FACILITIES AND POLLUTANTS New Hampshire Emissions from Existing Municipal Solid Waste Landfills § 62.7405 Identification of plan-negative declaration. On July 22, 1998, the New Hampshire Department of Environmental Services submitted a letter...

40 CFR 62.7405 - Identification of plan-negative declaration.

Code of Federal Regulations, 2010 CFR

2010-07-01

... PROGRAMS (CONTINUED) APPROVAL AND PROMULGATION OF STATE PLANS FOR DESIGNATED FACILITIES AND POLLUTANTS New Hampshire Emissions from Existing Municipal Solid Waste Landfills § 62.7405 Identification of plan-negative declaration. On July 22, 1998, the New Hampshire Department of Environmental Services submitted a letter...

40 CFR 62.7405 - Identification of plan-negative declaration.

Code of Federal Regulations, 2013 CFR

2013-07-01

... PROGRAMS (CONTINUED) APPROVAL AND PROMULGATION OF STATE PLANS FOR DESIGNATED FACILITIES AND POLLUTANTS New Hampshire Emissions from Existing Municipal Solid Waste Landfills § 62.7405 Identification of plan-negative declaration. On July 22, 1998, the New Hampshire Department of Environmental Services submitted a letter...

30 CFR 7.405 - Critical characteristics.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Cable Splice Kits § 7.405 Critical characteristics. (a) A sample from each production run, batch, or lot of manufactured electric cable, signaling cable, or splice made from a splice kit shall be flame... cable or splice and a sample of the cable or splice kit assembly shall be visually inspected or tested...

30 CFR 7.405 - Critical characteristics.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Cable Splice Kits § 7.405 Critical characteristics. (a) A sample from each production run, batch, or lot of manufactured electric cable, signaling cable, or splice made from a splice kit shall be flame... cable or splice and a sample of the cable or splice kit assembly shall be visually inspected or tested...

75 FR 36681 - Manufacturer of Controlled Substances Notice of Application

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-28

...-methylaminorex (cis isomer) (1590)....... I Alpha-ethyltryptamine (7249) I Lysergic acid diethylamide (7315) I 4-methylaminorex (cis isomer) (1590)....... I Alpha-ethyltryptamine (7249) I Lysergic acid diethylamide (7315) I 2... (7405). 4-methoxyamphetamine (7411) I Alpha-methyltryptamine (7432) I Bufotenine (7433) I...

76 FR 17969 - Manufacturer of Controlled Substances; Notice of Registration

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-31

... (2565) I Alpha-ethyltryptamine (7249) I Lysergic acid diethylamide (7315) I 2,5-Dimethoxy-4-(n)- I... I (7404). 3,4-Methylenedioxymethamphetamine (7405)... I 4-Methoxyamphetamine (7411) I Alpha...-propionoxypiperidine I (9661). Tilidine (9750) I Para-Fluorofentanyl (9812) I 3-Methylfentanyl (9813) I Alpha...

Simulating Astrophysical Jets with Inertial Confinement Fusion Machines

NASA Astrophysics Data System (ADS)

Blue, Brent

2005-10-01

Large-scale directional outflows of supersonic plasma, also known as `jets', are ubiquitous phenomena in astrophysics. The traditional approach to understanding such phenomena is through theoretical analysis and numerical simulations. However, theoretical analysis might not capture all the relevant physics and numerical simulations have limited resolution and fail to scale correctly in Reynolds number and perhaps other key dimensionless parameters. Recent advances in high energy density physics using large inertial confinement fusion devices now allow controlled laboratory experiments on macroscopic volumes of plasma of direct relevance to astrophysics. This talk will present an overview of these facilities as well as results from current laboratory astrophysics experiments designed to study hydrodynamic jets and Rayleigh-Taylor mixing. This work is performed under the auspices of the U. S. DOE by Lawrence Livermore National Laboratory under Contract No. W-7405-ENG-48, Los Alamos National Laboratory under Contract No. W-7405-ENG-36, and the Laboratory for Laser Energetics under Contract No. DE-FC03-92SF19460.

Modeling Supernova Shocks with Intense Lasers.

NASA Astrophysics Data System (ADS)

Blue, Brent

2006-04-01

Large-scale directional outflows of supersonic plasma are ubiquitous phenomena in astrophysics, with specific application to supernovae. The traditional approach to understanding such phenomena is through theoretical analysis and numerical simulations. However, theoretical analysis might not capture all the relevant physics and numerical simulations have limited resolution and fail to scale correctly in Reynolds number and perhaps other key dimensionless parameters. Recent advances in high energy density physics using large inertial confinement fusion devices now allow controlled laboratory experiments on macroscopic volumes of plasma of direct relevance to astrophysics. This talk will present an overview of these facilities as well as results from current laboratory astrophysics experiments designed to study hydrodynamic jets and Rayleigh-Taylor mixing. This work is performed under the auspices of the U. S. DOE by Lawrence Livermore National Laboratory under Contract No. W-7405-ENG-48, Los Alamos National Laboratory under Contract No. W-7405-ENG-36, and the Laboratory for Laser Energetics under Contract No. DE-FC03-92SF19460.

Automated Data Processing (ADP) Research and Development,

DTIC Science & Technology

1995-08-14

individual explosions were 16x16 ft for M1 and 18x18 ft for M2. 740 I L 1 tic 4 MI I f"hom~ \\fl i\\ 1l-2 t’lkercd li111c <, Crtc > jut!d WSHItlhZ ll cro...National Laboratory under contract W-7405-ENG-48. 733 1 . OBJECTIVES Our primary objective is to develop efficient and reliable automated event location and...real seismograms; Figure 1 shows example wavelet coefficients (in the transform domain) and bandpass filtering versions of a seismogram as a function of

Inertial Confinement Fusion as an Extreme Example of Dynamic Compression

NASA Astrophysics Data System (ADS)

Moses, E.

2013-06-01

Initiating and controlling thermonuclear burn at the national ignition facility (NIF) will require the manipulation of matter to extreme energy densities. We will discuss recent advances in both controlling the dynamic compression of ignition targets and our understanding of the physical states and processes leading to ignition. This work was performed under the auspices of the U.S. Department of Energy by Lawrence Livermore National Laboratory in part under Contract W-7405-Eng-48 and in part under Contract DE-AC52-07NA27344.

Asymmetric cell division requires specific mechanisms for adjusting global transcription.

PubMed

Mena, Adriana; Medina, Daniel A; García-Martínez, José; Begley, Victoria; Singh, Abhyudai; Chávez, Sebastián; Muñoz-Centeno, Mari C; Pérez-Ortín, José E

2017-12-01

Most cells divide symmetrically into two approximately identical cells. There are many examples, however, of asymmetric cell division that can generate sibling cell size differences. Whereas physical asymmetric division mechanisms and cell fate consequences have been investigated, the specific problem caused by asymmetric division at the transcription level has not yet been addressed. In symmetrically dividing cells the nascent transcription rate increases in parallel to cell volume to compensate it by keeping the actual mRNA synthesis rate constant. This cannot apply to the yeast Saccharomyces cerevisiae, where this mechanism would provoke a never-ending increasing mRNA synthesis rate in smaller daughter cells. We show here that, contrarily to other eukaryotes with symmetric division, budding yeast keeps the nascent transcription rates of its RNA polymerases constant and increases mRNA stability. This control on RNA pol II-dependent transcription rate is obtained by controlling the cellular concentration of this enzyme. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

First-Principle Simulations of Water

NASA Astrophysics Data System (ADS)

Schwegler, Eric

2004-03-01

The structural and dynamical properties of water are investigated with a series of first-principle and classical molecular dynamics simulations. A number of effects that can influence the simulated properties of water will be discussed including temperature, intramolecular flexibility [1], and the quantum nature of protons. This work was performed under the auspices of the U.S. Dept. of Energy at the University of California/Lawrence Livermore National Laboratory under contract no. W-7405-Eng-48. [1] M. Allesch, E. Schwegler, G. Galli, F. Gygi, J. Chem. Phys. in press 2004.

The Science of Cancer Prevention

Cancer.gov

The science of cancer prevention is described by Dr. Barnett S. Kramer, M.D., M.P.H., director of the Division of Cancer Prevention, National Cancer Institute (NCI). The Division of Cancer Prevention administers a broad spectrum of research that spans basic pre-clinical, laboratory research, supportive and palliative care research, early detection, and randomized controlled clinical trials. The Division also supports the Cancer Prevention Fellowship Program and is devoted to the balanced communication of scientific results.

Quantum Corral Wave-function Engineering

NASA Astrophysics Data System (ADS)

Correa, Alfredo; Reboredo, Fernando; Balseiro, Carlos

2005-03-01

We present a theoretical method for the design and optimization of quantum corrals[1] with specific electronic properties. Taking advantage that spins are subject to a RKKY interaction that is directly controlled by the scattering of the quantum corral, we design corral structures that reproduce spin Hamiltonians with coupling constants determined a priori[2]. We solve exactly the bi-dimensional scattering problem for each corral configuration within the s-wave approximation[3] and subsequently the geometry of the quantum corral is optimized by means of simulated annealing[4] and genetic algorithms[5]. We demonstrate the possibility of automatic design of structures with complicated target electronic properties[6]. This work was performed under the auspices of the US Department of Energy by the University of California at the LLNL under contract no W-7405-Eng-48. [1] M. F. Crommie, C. P. Lutz and D. M. Eigler, Nature 403, 512 (2000) [2] D. P. DiVincenzo et al., Nature 408, 339 (2000) [3] G. A. Fiete and E. J. Heller, Rev. Mod. Phys. 75, 933 (2003) [4] M. R. A. T. N. Metropolis et al., J. Chem. Phys. 1087 (1953) [5] E. Aarts and J. K. Lenstra, eds. Local search in combinatorial problems (Princeton University Press, 1997) [6] A. A. Correa, F. Reboredo and C. Balseiro, Phys. Rev. B (in press).

Control of Anion in Corporation in the Molecular Beam Epitaxy of Ternary Antimonide Superlattices for Very Long Wavelength Infrared Detection (Postprint)

DTIC Science & Technology

2015-10-01

ASSIGNED DISTRIBUTION STATEMENT. //Signature// //Signature// GAIL J. BROWN DIANA M. CARLIN, Chief Nanoelectronic ...Materials Branch Nanoelectronic Materials Branch Functional Materials Division Functional Materials Division //Signature// KAREN

The effect of desferrioxamine on transferrin receptors, the cell cycle and growth rates of human leukaemic cells.

PubMed Central

Bomford, A; Isaac, J; Roberts, S; Edwards, A; Young, S; Williams, R

1986-01-01

The effect of the iron chelator, desferrioxamine, on transferrin binding, growth rates and the cell cycle was investigated in the human leukaemic cell line, K562. At all concentrations of the chelator (2-50 microM) binding of 125I-transferrin was increased by 24 h and reached a maximum at 72-96 h. Maximum binding (6-8-fold increased) occurred in cells treated with 20 microM-desferrioxamine, in contrast with control cells which, at 96 h, showed a 50% decrease over initial binding. Scatchard analysis at 4 degrees C showed that this increased binding was due to an increase in the number of receptors, as the Kd was similar in induced (1.8 nM) and control (1.5 nM) cells. After 96 h cells, cultured with 20 and 50 microM-desferrioxamine accumulated 59Fe from bovine transferrin at over twice the rate found with control cells, reflecting the increase in transferrin receptors. Although iron uptake was unimpaired by the chelator there was a dose-dependent inhibition of cell growth, with control cells completing three divisions in 96 h and those in 10 microM-desferrioxamine only two divisions. At the highest concentration (50 microM), cell division was abrogated although cell viability was maintained (85%). In contrast, DNA synthesis was not markedly affected, except at 50 microM-desferrioxamine when incorporation of [3H]thymidine was 52% of that in control cells. Flow cytometry revealed that there was a progressive accumulation of the cells in the active phases of their cycle (S, G2 + M). Desferrioxamine may increase transferrin receptors in two ways: by chelating a regulatory pool of iron within the cell, and by arresting cells in S phase when receptors are maximally expressed. PMID:3790074

INSTRUMENTATION AND CONTROLS DIVISION, ELECTRICAL DESIGN STANDARDS AND GRAPHICAL SYMBOLS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bates, A.E.G.; Bowelle, M.M.; Horton, J.L.

1960-10-01

Recommendations of the Instrumentation and Controls Division Committee on Electrical and Electronic Symbols and Drawings are presented. The American Standards Associrtion Graphical Symbols for Electrical Diagrams are given, with certain variations or additions recommended by the Committee to clarify or more positively identify the device or element symbolized. Recommendations regarding electrical elementary diagram 1ayout, device coding, etc., are included. (W.D.M.)

12 CFR 740.5 - Requirements for the official advertising statement.

Code of Federal Regulations, 2013 CFR

2013-01-01

... advertising statement: (1) Credit union supplies such as stationery (except when used for circular letters..., including but not limited to, promotional items such as calendars, matchbooks, pens, pencils, and key chains...

12 CFR 740.5 - Requirements for the official advertising statement.

Code of Federal Regulations, 2014 CFR

2014-01-01

... advertising statement: (1) Credit union supplies such as stationery (except when used for circular letters..., including but not limited to, promotional items such as calendars, matchbooks, pens, pencils, and key chains...

12 CFR 740.5 - Requirements for the official advertising statement.

Code of Federal Regulations, 2012 CFR

2012-01-01

... advertising statement: (1) Credit union supplies such as stationery (except when used for circular letters..., including but not limited to, promotional items such as calendars, matchbooks, pens, pencils, and key chains...

15 CFR 740.5 - Civil end-users (CIV).

Code of Federal Regulations, 2010 CFR

2010-01-01

... end-uses in Country Group D:1, except North Korea (Supplement No. 1 to part 740 of this part). (b... for how to use STELA, or electronically from the Simplified Network Application Procedure (SNAP), see...

Coaxial Measurement of the Translational Distribution of CS Produced in the Laser Photolysis of CS2 at 193nm.

DTIC Science & Technology

1983-11-04

Division AREA & WORK UNIT NUMBERS . Department of Chemistry Howard University Washington, D. C. 20059 NR-051-733 1t. CONTROLLING OFFICE NAME AND...Journal of Physical Chemistry Laser Chemistry Division Department of Chemistry Howard University Washington, D. C. 20059 November 4, 1983 *Reproduction in...Victor McCrary, David Zakheim, and William M. Jackson Laser Chemistry Division Chemistry Departmient Howard University Washington, D.C.. 20059 ABSTRACT The

Controlling Cholesterol with Statins

MedlinePlus

... already in the blood. According to James P. Smith, M.D., M.S., deputy director of the Division ... the risk of heart disease and stroke,” says Smith. “But other factors that are out of our ...

40Gbit/s MDM-WDM Laguerre-Gaussian Mode with Equalization for Multimode Fiber in Access Networks

NASA Astrophysics Data System (ADS)

Fazea, Yousef; Amphawan, Angela

2018-04-01

Modal dispersion is seen as the primary impairment for multimode fiber. Mode division multiplexing (MDM) is a promising technology that has been realized as a favorable technology for considerably upsurges the capacity and distance of multimode fiber in conjunction with Wavelength Division Multiplexing (WDM) for fiber-to-the-home. This paper reveals the importance of an equalization technique in conjunction with controlling the modes spacing of mode division multiplexing-wavelength division multiplexing of Laguerre-Gaussian modes to alleviate modal dispersion for multimode fiber. The effects of channel spacing of 20 channels MDM-WDM were examined through controlling the azimuthal mode number and the radial mode number of Laguerre-Gaussian modes. A data rate of 40Gbit/s was achieved for a distance of 1,500 m for MDM-WDM.

LexA Binds to Transcription Regulatory Site of Cell Division Gene ftsZ in Toxic Cyanobacterium Microcystis aeruginosa.

PubMed

Honda, Takashi; Morimoto, Daichi; Sako, Yoshihiko; Yoshida, Takashi

2018-05-17

Previously, we showed that DNA replication and cell division in toxic cyanobacterium Microcystis aeruginosa are coordinated by transcriptional regulation of cell division gene ftsZ and that an unknown protein specifically bound upstream of ftsZ (BpFz; DNA-binding protein to an upstream site of ftsZ) during successful DNA replication and cell division. Here, we purified BpFz from M. aeruginosa strain NIES-298 using DNA-affinity chromatography and gel-slicing combined with gel electrophoresis mobility shift assay (EMSA). The N-terminal amino acid sequence of BpFz was identified as TNLESLTQ, which was identical to that of transcription repressor LexA from NIES-843. EMSA analysis using mutant probes showed that the sequence GTACTAN 3 GTGTTC was important in LexA binding. Comparison of the upstream regions of lexA in the genomes of closely related cyanobacteria suggested that the sequence TASTRNNNNTGTWC could be a putative LexA recognition sequence (LexA box). Searches for TASTRNNNNTGTWC as a transcriptional regulatory site (TRS) in the genome of M. aeruginosa NIES-843 showed that it was present in genes involved in cell division, photosynthesis, and extracellular polysaccharide biosynthesis. Considering that BpFz binds to the TRS of ftsZ during normal cell division, LexA may function as a transcriptional activator of genes related to cell reproduction in M. aeruginosa, including ftsZ. This may be an example of informality in the control of bacterial cell division.

Drive Scaling of hohlraums heated with 2ω light

NASA Astrophysics Data System (ADS)

Oades, Kevin; Foster, John; Slark, Gary; Stevenson, Mark; Kauffman, Robert; Suter, Larry; Hinkel, Denise; Miller, Mike; Schneider, Marilyn; Springer, Paul

2002-11-01

We report on experiments using a single beam from the AWE?s HELEN laser to study scaling of hohlraum drive with hohlraum scale size. The hohlruams were heated with 400 J in a 1 ns square pulse with and without a phaseplate. The drive was measured using a PCD and an FRD. Scattered light was measured using a full aperture backscatter system. Drive is consistent with hohlraum scaling and LASNEX modeling using the absorbed laser energy. Bremsstrahlung from fast electrons and M-shell x-ray production were also measured. This work was performed under the auspices of the U.S. Department of Energy by the University of California, Lawrence Livermore National Laboratory under contract No. W-7405-Eng-48.

National Program for Inspection of Non-Federal Dams. Millen Lake Dam (NH 00236), NHWRB Number 245.04, Connecticut River Basin, Washington, New Hampshire. Phase I Inspection Report.

DTIC Science & Technology

1980-03-01

Engineering Branch Engineering Division CARNEY M. TERZIAN, KENBER Design Branch Engineering Division RICHARD DIBKO CHIRA Water Control Branch...State of New Hampshire. Authorization and notice to proceed were issued to S E A Consultants Inc. under a letter of November 5, 1979 from William

High-spin states in the N=50 nucleus ^87Rb

NASA Astrophysics Data System (ADS)

Fotiades, N.; Cizewski, J. A.; Krücken, R.; Clark, R. M.; Fallon, P.; Lee, I. Y.; Macchiavelli, A. O.; Becker, J. A.; Bernstein, L. A.; McNabb, D. P.; Younes, W.

2001-10-01

High-spin states in ^87Rb have been studied following the fission of two compound nuclei (^199Tl and ^197Pb) formed in different fusion-evaporation reactions. The Gammasphere array at LBNL was used to detect γ-ray coincidences. The level scheme has been extended above the previously known 1578 keV, 9/2^+ isomer by observation of many states up to ~7.2 MeV excitation energy. Coupling of the odd g_9/2 proton to the yrast states in the ^86Kr core accounts for the first excited states observed above the 9/2^+ isomer. The level scheme of ^87Rb is also compared to excitations in ^85Kr and the ^89Y isotone. This work has been supported in part by the U.S. Department of Energy under Contracts No. W-7405-ENG-36 (LANL), FG02-91ER-40609 (Yale), W-7405-ENG-48 (LLNL) and AC03-76SF00098 (LBNL) and by the National Science Foundation (Rutgers).

Positron Annihilation Spectroscopy Characterization of Nanostructural Features in Reactor Steels

NASA Astrophysics Data System (ADS)

Glade, Stephen; Wirth, Brian; Asoka-Kumar, Palakkal; Sterne, Philip; Alinger, Matthew; Odette, George

2004-03-01

Irradiation embrittlement in nuclear reactor pressure vessel steels results from the formation of a high number density of nanometer sized copper rich precipitates and sub-nanometer defect-solute clusters. We present results of study to characterize the size and compositions of simple binary and ternary Fe-Cu-Mn model alloys and more representative Fe-Cu-Mn-Ni-Si-Mo-C reactor pressure vessel steels using positron annihilation spectroscopy (PAS). Using a recently developed spin-polarized PAS technique, we have also measured the magnetic properties of the nanometer-sized copper rich precipitates. Mn retards the precipitation kinetics and inhibits large vacancy cluster formation, suggesting a strong Mn-vacancy interaction which reduces radiation enhanced diffusion. The spin-polarized PAS measurements reveal the non-magnetic nature of the copper precipitates, discounting the notion that the precipitates contain significant quantities of Fe and providing an upper limit of at most a few percent Fe in the precipitates. PAS results on oxide dispersion-strengthened steel for use in fusion reactors will also be presented. Part of this work was performed under the auspices of the US Department of Energy by the University of California, Lawrence Livermore National Laboratory, under contract No. W-7405-ENG-48 with partial support provided from Basic Energy Sciences, Division of Materials Science.

75 FR 7405 - Airworthiness Directives; British Aerospace Regional Aircraft Model Jetstream Series 3101 and...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-19

... Airworthiness Directives; British Aerospace Regional Aircraft Model Jetstream Series 3101 and Jetstream Model... available in the AD docket shortly after receipt. FOR FURTHER INFORMATION CONTACT: Taylor Martin, Aerospace... AD docket. Relevant Service Information BAE Systems has issued British Aerospace Jetstream Series...

Characterization of dependencies between growth and division in budding yeast.

PubMed

Mayhew, Michael B; Iversen, Edwin S; Hartemink, Alexander J

2017-02-01

Cell growth and division are processes vital to the proliferation and development of life. Coordination between these two processes has been recognized for decades in a variety of organisms. In the budding yeast Saccharomyces cerevisiae , this coordination or 'size control' appears as an inverse correlation between cell size and the rate of cell-cycle progression, routinely observed in G 1 prior to cell division commitment. Beyond this point, cells are presumed to complete S/G 2 /M at similar rates and in a size-independent manner. As such, studies of dependence between growth and division have focused on G 1 Moreover, in unicellular organisms, coordination between growth and division has commonly been analysed within the cycle of a single cell without accounting for correlations in growth and division characteristics between cycles of related cells. In a comprehensive analysis of three published time-lapse microscopy datasets, we analyse both intra- and inter-cycle dependencies between growth and division, revisiting assumptions about the coordination between these two processes. Interestingly, we find evidence (i) that S/G 2 /M durations are systematically longer in daughters than in mothers, (ii) of dependencies between S/G 2 /M and size at budding that echo the classical G 1 dependencies, and (iii) in contrast with recent bacterial studies, of negative dependencies between size at birth and size accumulated during the cell cycle. In addition, we develop a novel hierarchical model to uncover inter-cycle dependencies, and we find evidence for such dependencies in cells growing in sugar-poor environments. Our analysis highlights the need for experimentalists and modellers to account for new sources of cell-to-cell variation in growth and division, and our model provides a formal statistical framework for the continued study of dependencies between biological processes. © 2017 The Author(s).

National Program for Inspection of Non-Federal Dams. Scovill Reservoir Dam (CT 00431), Lower Connecticut River Basin, Haddam, Connecticut. Phase I Inspection Report.

DTIC Science & Technology

1980-01-01

Engineering Branch Engineering Division CARNEY M. TERZIAN, MEMBER Design Branch Engineering Division S, RICHARD DIE O CHIRA Water Control Branch...Associates, P.C. under a letter of 19 October 1979 from William E. Hodgson, Jr., Colonel, Corps of Engineers. Contract No. DACW33-80-C-0001 has been assigned

Social Work with Religious Volunteers: Activating and Sustaining Community Involvement

ERIC Educational Resources Information Center

Garland, Diana R.; Myers, Dennis M.; Wolfer, Terry A.

2008-01-01

Social workers in diverse community practice settings recruit and work with volunteers from religious congregations. This article reports findings from two surveys: 7,405 congregants in 35 Protestant congregations, including 2,570 who were actively volunteering, and a follow-up survey of 946 volunteers. It compares characteristics of congregation…

12 CFR 740.5 - Requirements for the official advertising statement.

Code of Federal Regulations, 2011 CFR

2011-01-01

... statement until the supplies are exhausted. (b) The official advertising statement is in substance as... required to be published by state or federal law or regulation; (2) Credit union supplies such as..., matchbooks, pens, pencils, and key chains; (11) Advertisements that contain a statement to the effect that...

12 CFR 740.5 - Requirements for the official advertising statement.

Code of Federal Regulations, 2010 CFR

2010-01-01

... statement until the supplies are exhausted. (b) The official advertising statement is in substance as... required to be published by state or federal law or regulation; (2) Credit union supplies such as..., matchbooks, pens, pencils, and key chains; (11) Advertisements that contain a statement to the effect that...

30 CFR 740.5 - Definitions.

Code of Federal Regulations, 2010 CFR

2010-07-01

... lease means a Federal coal lease or license issued by the Bureau of Land Management pursuant to the... and part 745 of this chapter. Federal land management agency means a Federal agency having... lease bond means the bond or equivalent security required by 43 CFR part 3400 to assure compliance with...

Tidal impacts on the subtidal flow division at the main bifurcation in the Yangtze River Delta

NASA Astrophysics Data System (ADS)

Zhang, Wei; Feng, Haochuan; Hoitink, A. J. F.; Zhu, Yuliang; Gong, Fei; Zheng, Jinhai

2017-09-01

Flow division at bifurcations in the Yangtze Estuary has received ample attention, since it may control the pathways of terrestrial sediments over downstream river branches including the 12.5 m Deepwater Navigation channel. While some efforts have been made to interpret flow division at the bifurcations of the Yangtze Estuary, little attention has been paid to the role of tides. Flow division at estuarine bifurcations is made complicated by tides that propagate from the outlet of the tidal channels into the delta. To quantify the tidal influence on the distribution of river discharge, and more generally, to understand the mechanisms governing the subtidal flow division at the tidally affected bifurcation in the Yangtze River Delta, a two-dimensional hydrodynamic model is employed. In this model, the landward boundary is chosen beyond the tidal limit, where the tidal motion has faded out entirely. The seaward boundary is chosen such that the river discharge does not influence the water level. Subtidal discharges are decomposed using the method of factor separation, to distinguish between the effects of tides, river discharge and river-tide interactions on the subtidal flow division. Results indicate that tides modify the river discharge distribution over distributary channels in the Yangtze River Delta, particularly in the dry season. A significant difference in the subtidal flow division during spring tide and neap tide shows that the tidally averaged flow division over the distributaries in the delta greatly depends on tidal amplitude. By varying the river discharge at the landward boundary and amplitudes and phases of the principal tidal constituents at the seaward boundary of the established model, the sensitivities of the subtidal flow division to the river discharge and tidal amplitude variation were investigated in detail. Generally, the tidal impacts on the subtidal flow division are around 12% to 22%, with river discharge varying from 30,000 m3s-1 to 20,000 m3s-1. This effect on the flow distribution can even overwhelm the effects induced by river discharge based on geometry only, when the flow discharge is lowest. Furthermore, the fortnightly tidal cycle plays an important role in enhancing the inequality of the subtidal flow division caused by the M2 tidal component solely at the tidal bifurcation in the Yangtze River Delta during low flow.

Extraction of maxillary first molars improves second and third molar inclinations in Class II Division 1 malocclusion.

PubMed

Livas, Christos; Halazonetis, Demetrios J; Booij, Johan Willem; Katsaros, Christos

2011-09-01

The aim of this study was to assess the changes in inclination of the maxillary second (M2) and third (M3) molars after orthodontic treatment of Class II Division 1 malocclusion with extraction of maxillary first molars. Two groups of subjects were studied. The experimental group consisted of 37 subjects, 18 boys and 19 girls (mean age, 13.2 ± 1.62 years). The inclusion criteria were white origin, Class II Division 1 malocclusion, overjet ≥4 mm, no missing teeth or agenesis, and maxillary M3 present. All patients were treated with extraction of the maxillary first molars and the Begg technique. Standardized lateral cephalometric radiographs were taken at the start of active treatment (T1) and at least 3.7 years posttreatment (T2). The control group was drawn from the archives of the Nittedal Growth Material (Oslo University, Oslo, Norway) and included 54 untreated Class I and Class II subjects,18 boys and 36 girls (mean age, 13.4 ± 1.99 years) followed up for a minimum of 3.6 years. M2 and M3 inclinations relative to the palatal plane (PP) and functional occlusal plane (FOP) were measured and compared between groups and time periods. M2 to PP inclination improved significantly in both the control group (M2-PP at T1, 17.7° ± 5.81°, and at T2, 11.9° ± 4.61°) and the experimental group (M2-PP at T1, 26.7° ± 5.75°, and at T2, 6.9° ± 6.76°). There were also significant increases of the mesial inclination of M3 in the control group (M3-PP at T1, 30.1° ± 8.54°, and at T2, 19.6° ± 9.01°) and extraction group (M3-PP at T1, 32.2° ± 7.90°, and at T2, 12.8° ± 7.36°). By using the FOP as the reference system, no significant change in the inclination of M2 was observed in the control group, whereas, in the extraction group, although more distally inclined at T1, M2 ended up mesially inclined at T2 (M2-FOP at T1, 14.2° ± 4.62°, and at T2, -6.2° ± 6.10°; P <0.0001). M3 inclinations were similar between the groups at T1 (M3-FOP control, 17.3° ± 9.35°; M3-FOP experimental, 19.6° ± 7.37°), and these improved significantly in both groups. However, M3 uprighting was almost 4 times greater in the extraction group (M3-FOP from T2-T1, 5.6° vs 19.9°). The greatest distal inclination of M3 at T2 in the extraction group was 9.4°, a value attained by only 43% of the control group. Extraction of the maxillary first molars in Class II Division 1 patients results in significant uprighting of M2 and M3 and facilitates the normal eruption of M3. Copyright © 2011 American Association of Orthodontists. Published by Mosby, Inc. All rights reserved.

On the Feasibility of Very-Low-Density Pure Metal Foams as Bright High-Energy X-ray Sources

NASA Astrophysics Data System (ADS)

Colvin, Jeffrey; Felter, Thomas

2003-10-01

We have used the Busquet approximation (M. Busquet, Phys. Fluids B 5(11), 4191 (1993)) to explore calculationally what the possible x-ray conversion efficiencies into the K-band would be from irradiating very-low-density pure metal foams with tens of kilojoules of 1/3-micron laser light. We will discuss the advantages of pure metal foams as bright high-energy x-ray sources, and some results of this calculational study. We will also present our ideas for how to fabricate pure metal foams with densities of a few milligrams per cubic centimeter. This work was performed under the auspices of the US Department of Energy by University of California Lawrence Livermore National Laboratory under contract No. W-7405-Eng-48.

75 FR 16843 - Core Manufacturing, Multi-Plastics, Inc., Division, Sipco, Inc., Division, Including Leased...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-02

... Manufacturing, Multi-Plastics, Inc., Division, Sipco, Inc., Division, Including Leased Workers of M-Ploy... Manufacturing, Multi-Plastics, Inc., Division and Sipco, Inc., Division, including leased workers of M-Ploy... applicable to TA-W-70,457 is hereby issued as follows: ``All workers of Core Manufacturing, Multi-Plastics...

Muscarinic receptor binding increases in anterior thalamus and cingulate cortex during discriminative avoidance learning

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vogt, B.A.; Gabriel, M.; Vogt, L.J.

Training-induced neuronal activity develops in the mammalian limbic system during discriminative avoidance conditioning. This study explores behaviorally relevant changes in muscarinic ACh receptor binding in 52 rabbits that were trained to one of five stages of conditioned response acquisition. Sixteen naive and 10 animals yoked to criterion performance served as control cases. Upon reaching a particular stage of training, the brains were removed and autoradiographically assayed for 3H-oxotremorine-M binding with 50 nM pirenzepine (OxO-M/PZ) or for 3H-pirenzepine binding in nine limbic thalamic nuclei and cingulate cortex. Specific OxO-M/PZ binding increased in the parvocellular division of the anterodorsal nucleus early inmore » training when the animals were first exposed to pairing of the conditional and unconditional stimuli. Elevated binding in this nucleus was maintained throughout subsequent training. In the parvocellular division of the anteroventral nucleus (AVp), OxO-M/PZ binding progressively increased throughout training, reached a peak at the criterion stage of performance, and returned to control values during extinction sessions. Peak OxO-M/PZ binding in AVp was significantly elevated over that for cases yoked to criterion performance. In the magnocellular division of the anteroventral nucleus (AVm), OxO-M/PZ binding was elevated only during criterion performance of the task, and it was unaltered in any other limbic thalamic nuclei. Specific OxO-M/PZ binding was also elevated in most layers in rostral area 29c when subjects first performed a significant behavioral discrimination. Training-induced alterations in OxO-M/PZ binding in AVp and layer Ia of area 29c were similar and highly correlated.« less

Preparation and certification of hijiki reference material, NMIJ CRM 7405-a, from the edible marine algae hijiki (Hizikia fusiforme).

PubMed

Narukawa, Tomohiro; Inagaki, Kazumi; Zhu, Yanbei; Kuroiwa, Takayoshi; Narushima, Izumi; Chiba, Koichi; Hioki, Akiharu

2012-02-01

A certified reference material, NMIJ CRM 7405-a, for the determination of trace elements and As(V) in algae was developed from the edible marine hijiki (Hizikia fusiforme) and certified by the National Metrology Institute of Japan (NMIJ), the National Institute of Advanced Industrial Science and Technology (AIST). Hijiki was collected from the Pacific coast in the Kanto area of Japan, and was washed, dried, powdered, and homogenized. The hijiki powder was placed in 400 bottles (ca. 20 g each). The concentrations of 18 trace elements and As(V) were determined by two to four independent analytical techniques, including (ID)ICP-(HR)MS, ICP-OES, GFAAS, and HPLC-ICP-MS using calibration solutions prepared from the elemental standard solution of Japan calibration service system (JCSS) and the NMIJ CRM As(V) solution, and whose concentrations are certified and SI traceable. The uncertainties of all the measurements and preparation procedures were evaluated. The values of 18 trace elements and As(V) in the CRM were certified with uncertainty (k = 2).

Solvation of Na^+ in water from first-principles molecular dynamics

NASA Astrophysics Data System (ADS)

White, J. A.; Schwegler, E.; Galli, G.; Gygi, F.

2000-03-01

We have carried out ab initio molecular dynamics (MD) simulations of the Na^+ ion in water with an MD cell containing a single alkali ion and 53 water molecules. The electron-electron and electron-ion interactions were modeled by density functional theory with a generalized gradient approximation for the exchange-correlation functional. The computed radial distribution functions, coordination numbers, and angular distributions are consistent with available experimental data. The first solvation shell contains 5.2±0.6 water molecules, with some waters occasionally exchanging with those of the second shell. The computed Na^+ hydration number is larger than that from calculations for water clusters surrounding an Na^+ ion, but is consistent with that derived from x-ray measurements. Our results also indicate that the first hydration shell is better defined for Na^+ than for K^+ [1], as indicated by the first minimum in the Na-O pair distribution function. [1] L.M. Ramaniah, M. Bernasconi, and M. Parrinello, J. Chem. Phys. 111, 1587 (1999). This work was performed for DOE under contract W-7405-ENG-48.

77 FR 73667 - National Cancer Institute; Notice of Closed Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-11

... Behavioral Research in Cancer Control. Date: January 9-10, 2013. Time: 10:00 a.m. to 2:00 p.m. Agenda: To..., Research Programs Review Branch, Division of Extramural Activities, National Cancer Institute, NIH, 6116... Committee: National Cancer Institute Special Emphasis Panel; Quantitative Imaging for the Evaluation of...

TfVPS32 Regulates Cell Division in the Parasite Tritrichomonas foetus.

PubMed

Iriarte, Lucrecia S; Midlej, Victor; Frontera, Lorena S; Moros Duarte, Daniel; Barbeito, Claudio G; de Souza, Wanderley; Benchimol, Marlene; de Miguel, Natalia; Coceres, Veronica M

2018-01-01

The flagellated protist Tritrichomonas foetus is a parasite that causes bovine trichomonosis, a major sexually transmitted disease in cattle. Cell division has been described as a key player in controlling cell survival in other cells, including parasites but there is no information on the regulation of this process in T. foetus. The regulation of cytokinetic abscission, the final stage of cell division, is mediated by members of the ESCRT (endosomal sorting complex required for transport) machinery. VPS32 is a subunit within the ESCRTIII complex and here, we report that TfVPS32 is localized on cytoplasmic vesicles and a redistribution of the protein to the midbody is observed during the cellular division. In concordance with its localization, deletion of TfVPS32 C-terminal alpha helices (α5 helix and/or α4-5 helix) leads to abnormal T. foetus growth, an increase in the percentage of multinucleated parasites and cell cycle arrest at G2/M phase. Together, these results indicate a role of this protein in controlling normal cell division. © 2017 The Author(s) Journal of Eukaryotic Microbiology © 2017 International Society of Protistologists.

Activity in the Mission Control Center during Apollo 14

NASA Image and Video Library

1971-02-04

S71-17610 (4 Feb. 1971) --- Partial view of activity in the Mission Operations Control Room in the Mission Control Center at the time the Apollo 14 S-IVB stage impacted on the lunar surface. The flight director's console is in the foreground. Eugene F. Kranz, chief of the MSC Flight Control Division, is in the right foreground. Seated at the console is Glynn S. Lunney, head of the Flight Director Office, Flight Control Division. Facing the camera is Gerald D. Griffin, flight director of the Third (Gold) Team. A seismic reading from the impact can be seen in the center background. The S-IVB impacted on the lunar surface at 1:40:54 a.m. (CST), Feb. 4, 1971, about 90 nautical miles south-southwest of the Apollo 12 passive seismometer. The energy release was comparable to 11 tons of TNT.

78 FR 49550 - Meetings of Humanities Panel

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-14

... Division of Education Programs. 2. Date: September 09, 2013. Time: 8:30 a.m. to 5:00 p.m. Room: 421. This... Initiatives at Tribal Colleges and Universities grant program, submitted to the Division of Education Programs... Division of Education Programs. 6. Date: September 12, 2013. Time: 8:30 a.m. to 5:00 p.m. Room: 421. This...

75 FR 425 - Extension of Approved Information Collection, OMB Control Number 1004-0073

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-05

... be available for public review at the L Street address during regular business hours (7:45 a.m. to 4:15 p.m.), Monday through Friday, excluding Federal holidays. FOR FURTHER INFORMATION CONTACT: You may contact John A. Lewis, Division of Solid Minerals at (202) 912-7116 (Commercial or FTS). Persons who use a...

Instability Coupling Experiments*

NASA Astrophysics Data System (ADS)

Chrien, R. E.; Hoffman, N. M.; Magelssen, G. R.; Schappert, G. T.; Smitherman, D. P.

1996-11-01

The coupling of Richtmyer-Meshkov (RM) and ablative Rayleigh-Taylor (ART) instabilities is being studied with indirectly-driven planar foil experiments on the Nova laser at Livermore. The foil is attached to a 1.6-mm-diameter, 2.75-mm-long Au hohlraum driven by a 2.2-ns long, 1:5-contrast-ratio shaped laser pulse. A shock is generated in 35-μm or 86-μm thick Al foils with a 50-μm-wavelength, 4-μm-amplitude sinusoidal perturbation on its rear surface. In some experiments, the perturbation is applied to a 10-μm Be layer on the Al. A RM instability develops when the shock encounters the perturbed surface. The flow field of the RM instability can ``feed out'' to the ablation surface of the foil and provide the seed for ART perturbation growth. Face-on and side-on x-radiography are used to observe areal density perturbations in the foil. For the 86-μm foil, the perturbation arrives at the ablation surface while the hohlraum drive is dropping and the data are consistent with RM instability alone. For the 35-μm foil, the perturbation feeds out while the hohlraum drive is close to its peak and the data appear to show strong ART perturbation growth. Comparisons with LASNEX simulations will be presented. *This work supported under USDOE contract W-7405-ENG-36.

Implications of Organizational Planning for Crisis Relocation.

DTIC Science & Technology

1982-12-01

AD-A 23 956 IMPLICATIONS OF ORGANIZATIDNAL PLANNING FOR CRISIS RELOCATION(U) NORTH CAROLINA DEPT OF CRIME CONTROL AND PUBLIC SAFETY RALEIG.. M A...policies of the Federal Emergency Management Agency. Division of Emergency Management North Carolina Department of Crime Control and Public Safety...North Carolina Department of Crime Control and Work Unit 0 4412 1 Public Safety, 116 W.Jones St. ,Raleigh, NC 27611 II. CONTROLLING OFFICE NAME AND

Regulation of the expression of the cell-cycle gene ftsZ by DicF antisense RNA. Division does not require a fixed number of FtsZ molecules.

PubMed

Tétart, F; Bouché, J P

1992-03-01

We show that the 53-nucleotide RNA molecule encoded by gene dicF blocks cell division in Escherichia coli by inhibiting the translation of ftsZ mRNA. Such a role for dicF had been predicted on the basis of the complementarity of DicF RNA with the ribosome-binding region of the ftsZ mRNA. An analysis of ftsZ expression at its chromosomal locus, and of an ftsZ-lacZ translational fusion controlled by promoters ftsZ1p and ftsZ2p only, indicates that ftsZ is not autoregulated. Partial inhibition of FtsZ synthesis leads to increased cell size. However, the number of FtsZ molecules per cell can be reduced threefold without affecting the division rate significantly. Our results suggest that septation is not triggered by a fixed number of newly synthesized FtsZ molecules per cell.

76 FR 81986 - Honeywell International, Inc., Automation and Control Solutions Division, Including On-Site...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-29

..., Inc., Automation and Control Solutions Division, Including On-Site Leased Workers From Manpower... International, Inc., Automation and Control Solutions Division, Rock Island, Illinois. The notice was published...., Automation and Control Solutions Division. The Department has determined that these workers were sufficiently...

Recommended Reading List for Faculty and Students, Military Qualification Standards (MQS) II Reading List.

DTIC Science & Technology

1983-01-01

1950s. R 6 Saxe, Comte Maurice de. Reveries on the Art of Wa- (1944). U 101 A brilliant military theorist, Maurice de Sase believed that warfare, like... Agust 1972. 4 16 Middleton. Drew. The Duel at the it: China and Russia iAia(1978). DS 740.5 Th. book chronicles te authr 1, *rSalons of his trip t ino

Method of improving superconducting qualities of fabricated constructs by shock preprocessing of precursor materials

DOEpatents

Nellis, William J.; Maple, M. Brian

1992-01-01

Disclosed is a method of improving the physical properties of superconducting materials which comprises: a. applying a high strain rate deformation to said materi The United States Government has rights in this invention pursuant to Contract No. W-7405-ENG-48 between the U.S. Department of Energy and the University of California, for the operation of Lawrence Livermore National Laboratory.

National Program for Inspection of Non-Federal Dams. Keyser Dam (VT 00097), Connecticut River Basin, Chelsea, Vermont. Phase I Inspection Report.

DTIC Science & Technology

1980-04-01

CARNEY M. TERZIAN, HENBER I Design Branch Engineering Division RICHARD DIB * Water Control Branch Engineering Division [ hPIPWVAL 220ininu: Chief...2 f. Operator 2 I g. Purpose of Dam 2 h. Design and Construction History 2 i. Normal Operational Procedure 2 1.3 Pertinent Data 2 a. Drainage...i. Spillway 5 J. Regulating Outlets 5 [I h] Section Page 2. ENGINEERING DATA 6 2.1 Design Data 6 2.2 Construction Data 6 2.3 Operation Data 6 2.4

Dynamic Stability of Maglev Systems,

DTIC Science & Technology

1992-04-01

AD-A259 178 ANL-92/21 Materials and Components Dynamic Stability of Technology Division Materials and Components Maglev Systems Technology Division...of Maglev Systems Y. Cai, S. S. Chen, and T. M. Mulcahy Materials and Components Technology Division D. M. Rote Center for Transportation Research...of Maglev System with L-Shaped Guideway ......................................... 6 3 Stability of M aglev System s

Vehicle/Guideway Interaction in Maglev Systems

DTIC Science & Technology

1992-03-01

Technology Division Materials and Components in Maglev Systems Technology Division Materials and Components Technology Division byY. Cai, S. S. Chen, and D. M...Transportation Systems Reports (UC-330, Vehicle/Guideway Interaction in Maglev Systems by Y. Cai and S. S. Chen Materials and Components Technology Division D. M...Surface Irregularities ...................................... 32 4 Vehicle/Guideway Interaction in Transrapid Maglev System .................. 34 4.1

46 CFR 108.173 - Class I, Division 2 locations.

Code of Federal Regulations, 2011 CFR

2011-10-01

... m (10 ft.) of a possible source of gas release; or (3) Within 1.5 m (5 ft.) of the boundaries of any...) Within 1.5 m (5 ft.) of a semi-enclosed Class I, Division 1 location indicated in § 108.171(b); or (2) Within 1.5 m (5 ft.) of a Class I, Division 1 space indicated in § 108.171(e). (d) A semi-enclosed area...

46 CFR 108.173 - Class I, Division 2 locations.

Code of Federal Regulations, 2013 CFR

2013-10-01

... m (10 ft.) of a possible source of gas release; or (3) Within 1.5 m (5 ft.) of the boundaries of any...) Within 1.5 m (5 ft.) of a semi-enclosed Class I, Division 1 location indicated in § 108.171(b); or (2) Within 1.5 m (5 ft.) of a Class I, Division 1 space indicated in § 108.171(e). (d) A semi-enclosed area...

46 CFR 108.173 - Class I, Division 2 locations.

Code of Federal Regulations, 2012 CFR

2012-10-01

... m (10 ft.) of a possible source of gas release; or (3) Within 1.5 m (5 ft.) of the boundaries of any...) Within 1.5 m (5 ft.) of a semi-enclosed Class I, Division 1 location indicated in § 108.171(b); or (2) Within 1.5 m (5 ft.) of a Class I, Division 1 space indicated in § 108.171(e). (d) A semi-enclosed area...

46 CFR 108.173 - Class I, Division 2 locations.

Code of Federal Regulations, 2014 CFR

2014-10-01

... m (10 ft.) of a possible source of gas release; or (3) Within 1.5 m (5 ft.) of the boundaries of any...) Within 1.5 m (5 ft.) of a semi-enclosed Class I, Division 1 location indicated in § 108.171(b); or (2) Within 1.5 m (5 ft.) of a Class I, Division 1 space indicated in § 108.171(e). (d) A semi-enclosed area...

Health hazard evaluation report heta 92-0160-2360, City of Lancaster, Division of Fire, Lancaster, Ohio

DOE Office of Scientific and Technical Information (OSTI.GOV)

Echt, A.; Blade, L.; Sheehy, J.

1993-10-01

In response to a request from the Division of Fire and the International Association of Firefighters Local 291, an evaluation was undertaken of exposure to diesel exhaust emissions in the engine houses of the City of Lancaster Division of Fire (SIC-9224) Lancaster, Ohio. At the time of the study, 77 uniformed employees and two secretaries worked in three engine houses. In Engine House 1, personal breathing zone (PBZ) samples ranged from 51.7 to 71.2 micrograms per cubic meter (microg/cu m) on the first night of sampling. PBZ samples in Engine House 2 ranged from 25.7 to 78.8 microg/cu m overmore » two nights of sampling. Results for Engine House 3 ranged from 24.0 to 60.5 microg/cu m. With the exception of one measurement of 4.6 parts per million (ppm) of carbon-monoxide (CO) measured in the smoking room of Engine House 2, only trace CO was detected. Benzene solubles measured in Engine House 2 ranged from less than the limit of detection to 313 microg/cu m. The authors conclude that exhaust emissions containing diesel particulate may present a potential risk to firefighters. The authors recommend the use of engineering controls and work practices to reduce diesel exhaust emission exposures.« less

78 FR 73520 - Agency Information Collection Activities; Proposed Collection; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-06

... Pollution Prevention and Toxics (OPPT), Environmental Protection Agency, 1200 Pennsylvania Ave. NW..., Exposure and Technology Division (7406M), Office of Pollution Prevention and Toxics, Environmental... appropriate to control exposures to lead dust resulting from renovation, repair, and painting (RRP) activities...

Glucose Regulates Cyclin D2 Expression in Quiescent and Replicating Pancreatic β-Cells Through Glycolysis and Calcium Channels

PubMed Central

Salpeter, Seth J.; Klochendler, Agnes; Weinberg-Corem, Noa; Porat, Shay; Granot, Zvi; Shapiro, A. M. James; Magnuson, Mark A.; Eden, Amir; Grimsby, Joseph; Glaser, Benjamin

2011-01-01

Understanding the molecular triggers of pancreatic β-cell proliferation may facilitate the development of regenerative therapies for diabetes. Genetic studies have demonstrated an important role for cyclin D2 in β-cell proliferation and mass homeostasis, but its specific function in β-cell division and mechanism of regulation remain unclear. Here, we report that cyclin D2 is present at high levels in the nucleus of quiescent β-cells in vivo. The major regulator of cyclin D2 expression is glucose, acting via glycolysis and calcium channels in the β-cell to control cyclin D2 mRNA levels. Furthermore, cyclin D2 mRNA is down-regulated during S-G2-M phases of each β-cell division, via a mechanism that is also affected by glucose metabolism. Thus, glucose metabolism maintains high levels of nuclear cyclin D2 in quiescent β-cells and modulates the down-regulation of cyclin D2 in replicating β-cells. These data challenge the standard model for regulation of cyclin D2 during the cell division cycle and suggest cyclin D2 as a molecular link between glucose levels and β-cell replication. PMID:21521747

Concerted control of Escherichia coli cell division

PubMed Central

Osella, Matteo; Nugent, Eileen; Cosentino Lagomarsino, Marco

2014-01-01

The coordination of cell growth and division is a long-standing problem in biology. Focusing on Escherichia coli in steady growth, we quantify cell division control using a stochastic model, by inferring the division rate as a function of the observable parameters from large empirical datasets of dividing cells. We find that (i) cells have mechanisms to control their size, (ii) size control is effected by changes in the doubling time, rather than in the single-cell elongation rate, (iii) the division rate increases steeply with cell size for small cells, and saturates for larger cells. Importantly, (iv) the current size is not the only variable controlling cell division, but the time spent in the cell cycle appears to play a role, and (v) common tests of cell size control may fail when such concerted control is in place. Our analysis illustrates the mechanisms of cell division control in E. coli. The phenomenological framework presented is sufficiently general to be widely applicable and opens the way for rigorous tests of molecular cell-cycle models. PMID:24550446

Similar effects of substance P on learning and memory function between hippocampus and striatal marginal division

PubMed Central

Yu, Yan; Zeng, Changchun; Shu, Siyun; Liu, Xuemei; Li, Chuhua

2014-01-01

Substance P is an endogenous neurokinin that is present in the central and peripheral nervous systems. The neuropeptide substance P and its high-affinity receptor neurokinin 1 receptor are known to play an important role in the central nervous system in inflammation, blood pressure, motor behavior and anxiety. The effects of substance P in the hippocampus and the marginal division of the striatum on memory remain poorly understood. Compared with the hippocampus as a control, immunofluorescence showed high expression of the substance P receptor, neurokinin 1, in the marginal division of the striatum of normal rats. Unilateral or bilateral injection of an antisense oligonucleotide against neurokinin 1 receptor mRNA in the rat hippocampus or marginal division of the striatum effectively reduced neurokinin 1 receptor expression. Independent of injection site, rats that received this antisense oligonucleotide showed obviously increased footshock times in a Y-maze test. These results indicate that the marginal division of the striatum plays a similar function in learning and memory to the hippocampus, which is a valuable addition to our mechanistic understanding of the learning and memory functions of the marginal division of the striatum. PMID:25206901

77 FR 49450 - National Cancer Institute; Notice of Closed Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-16

... Committee: National Cancer Institute Special Emphasis Panel; Core Infrastructure and Methodological Research...; Small Grants for Behavioral Research in Cancer Control. Date: September 18-19, 2012.. Time: 9 a.m. to 12...., Scientific Review Officer, Research Programs Review Branch, Division of Extramural Activities, National...

76 FR 576 - National Cancer Institute; Notice of Closed Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-05

..., Cancer Centers Support; 93.398, Cancer Research Manpower; 93.399, Cancer Control, National Institutes of...: Wlodek Lopaczynski, M.D., PhD, Scientific Review Officer, Research Programs Review Branch, Division of... Institute Special Emphasis Panel; Collaborative Research in Integrative Cancer Biology and the Tumor...

Method and apparatus for secondary laser pumping by electron beam excitation

DOEpatents

George, E. Victor; Krupke, William F.; Murray, John R.; Powell, Howard T.; Swingle, James C.; Turner, Jr., Charles E.; Rhodes, Charles K.

1978-01-01

An electron beam of energy typically 100 keV excites a fluorescer gas which emits ultraviolet radiation. This radiation excites and drives an adjacent laser gas by optical pumping or photolytic dissociation to produce high efficiency pulses. The invention described herein was made in the course of, or under, United States Energy Research and Development Administration Contract No. W-7405-Eng-48 with the University of California.

’Do-It-Yourself’ Fallout/Blast Shelter Evaluation

DTIC Science & Technology

1984-03-01

N4AME & AOORIESS(I! dittvrevI !M’", Controlling Olif~t) IS. SEC’.JRITY CL-ASS. (GO this report) Lawrence Livermore National Laboratory Unclassified P...the data from the transient recorder iemory tirough the Computer Automated Measurement and Control (CAMAC) data busa und stores them on an $-inch...Command and Control Technical Center Emergency Technology Division Department of Defense 0a& Ridge Natioual Laboratory The Pentagon Attn: Librarian

INSTRUMENTATION AND CONTROLS DIVISION ELECTRICAL DESIGN STANDARDS AND GRAPHICAL SYMBOLS

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

1961-11-01

Copies are presented of the American Standards Association graphical symbols for electrical and electronic equipment and systems. Recommendations are given for electrical elementary design layout, device codings, etc., for permanent type installations. Electrical diagrams copied from American Drafting Standards Manual are presented. (M.C.G.)

76 FR 51970 - Agency Information Collection Activities; Proposed Collection; Comment Request; Underground...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-19

... recordkeeping burden for this collection of information is estimated to average 2.32 hours per response. Burden... . FOR FURTHER INFORMATION CONTACT: Robert E. Smith, Office of Ground Water and Drinking Water/Drinking Water Protection Division/Underground Injection Control Program, Mailcode: 4606M, Environmental...

Low-latency optical parallel adder based on a binary decision diagram with wavelength division multiplexing scheme

NASA Astrophysics Data System (ADS)

Shinya, A.; Ishihara, T.; Inoue, K.; Nozaki, K.; Kita, S.; Notomi, M.

2018-02-01

We propose an optical parallel adder based on a binary decision diagram that can calculate simply by propagating light through electrically controlled optical pass gates. The CARRY and CARRY operations are multiplexed in one circuit by a wavelength division multiplexing scheme to reduce the number of optical elements, and only a single gate constitutes the critical path for one digit calculation. The processing time reaches picoseconds per digit when we use a 100-μm-long optical path gates, which is ten times faster than a CMOS circuit.

Physalis floridana Cell Number Regulator1 encodes a cell membrane-anchored modulator of cell cycle and negatively controls fruit size

PubMed Central

Li, Zhichao; He, Chaoying

2015-01-01

Physalis species show a significant variation in berry size; however, the underlying molecular basis is unknown. In this work, we showed that cell division difference in the ovaries might contribute to the ultimate berry size variation within Physalis species, and that mRNA abundance of Physalis floridana Cell Number Regulator1 (PfCNR1), the putative orthologue of the tomato fruit weight 2.2 (FW2.2), was negatively correlated with cell division in the ovaries. Moreover, heterochronic expression variation of the PfCNR1 genes in the ovaries concomitantly correlated with berry weight variation within Physalis species. In transgenic Physalis, multiple organ sizes could be negatively controlled by altering PfCNR1 levels, and cell division instead of cell expansion was primarily affected. PfCNR1 was shown to be anchored in the plasma membrane and to interact with PfAG2 (an AGAMOUS-like protein determining ovary identity). The expression of PfCYCD2;1, a putative orthologue of the mitosis-specific gene CyclinD2;1 in the cell cycle was negatively correlated with the PfCNR1 mRNA levels. PfAG2 was found to selectively bind to the CArG-box in the PfCYCD2;1 promoter and to repress PfCYCD2;1 expression, thus suggesting a PfAG2-mediated pathway for PfCNR1 to regulate cell division. The interaction of PfCNR1 with PfAG2 enhanced the repression of PfCYCD2;1 expression. The nuclear import of PfAG2 was essential in the proposed pathway. Our data provide new insights into the developmental pathways of a cell membrane-anchored protein that modulates cell division and governs organ size determination. This study also sheds light on the link between organ identity and organ growth in plants. PMID:25305759

A biologically based model of growth and senescence of Syrian hamster embryo (SHE) cells after exposure to arsenic.

PubMed Central

Liao, K H; Gustafson, D L; Fox, M H; Chubb, L S; Reardon, K F; Yang, R S

2001-01-01

We modified the two-stage Moolgavkar-Venzon-Knudson (MVK) model for use with Syrian hamster embryo (SHE) cell neoplastic progression. Five phenotypic stages are proposed in this model: Normal cells can either become senescent or mutate into immortal cells followed by anchorage-independent growth and tumorigenic stages. The growth of normal SHE cells was controlled by their division, death, and senescence rates, and all senescent cells were converted from normal cells. In this report, we tested the modeling of cell kinetics of the first two phenotypic stages against experimental data evaluating the effects of arsenic on SHE cells. We assessed cell division and death rates using flow cytometry and correlated cell division rates to the degree of confluence of cell cultures. The mean cell death rate was approximately equal to 1% of the average division rate. Arsenic did not induce immortalization or further mutations of SHE cells at concentrations of 2 microM and below, and chromium (3.6 microM) and lead (100 microM) had similar negative results. However, the growth of SHE cells was inhibited by 5.4 microM arsenic after a 2-day exposure, with cells becoming senescent after only 16 population doublings. In contrast, normal cells and cells exposed to lower arsenic concentrations grew normally for at least 30 population doublings. The biologically based model successfully predicted the growth of normal and arsenic-treated cells, as well as the senescence rates. Mechanisms responsible for inducing cellular senescence in SHE cells exposed to arsenic may help explain the apparent inability of arsenic to induce neoplasia in experimental animals. PMID:11748027

Reducing risk of Anthracycline-related heart failure after childhood cancer | Division of Cancer Prevention

Cancer.gov

Childhood cancer survivors are at a 15-fold risk of developing heart failure (HF) compared to age-matched controls. There is a strong dose-dependent association between anthracyclines and risk of HF; the incidence approaches 20% at cumulative doses between 300-600 mg/m2, and exceeds 30% for doses >600 mg/m2. Outcome following HF is poor; 5-year survival rate is |

77 FR 22359 - Meetings of Humanities Panel

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-13

... Institutes grant program, submitted to the Division of Education Programs, at the March 1, 2012 deadline. 2... The Summer Seminars and Institutes grant program, submitted to the Division of Education Programs, at... Division of Education Programs, at the March 1, 2012 deadline. 4. Date: May 7, 2012. Time: 9 a.m. to 5 p.m...

77 FR 40638 - Alternative Staffing, Formerly Known as First Choice Staffing, Working On-Site at Ametek...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-10

..., Instrumentation and Speciality Controls Division, West Chicago, IL; Amended Certification Regarding Eligibility To... Nationals Controls Corporation, Instrumentation and Specialty Control Division, West Chicago, Illinois. The... Corporation, Instrumentation and Specialty Control Division, West Chicago, Illinois, separated from employment...

33 CFR 274.6 - Division/district pest control programs.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 33 Navigation and Navigable Waters 3 2013-07-01 2013-07-01 false Division/district pest control..., DEPARTMENT OF DEFENSE PEST CONTROL PROGRAM FOR CIVIL WORKS PROJECTS Project Operation § 274.6 Division/district pest control programs. (a) Guides. Referenced technical manuals, and Engineer Circulars issued...

33 CFR 274.6 - Division/district pest control programs.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 33 Navigation and Navigable Waters 3 2012-07-01 2012-07-01 false Division/district pest control..., DEPARTMENT OF DEFENSE PEST CONTROL PROGRAM FOR CIVIL WORKS PROJECTS Project Operation § 274.6 Division/district pest control programs. (a) Guides. Referenced technical manuals, and Engineer Circulars issued...

33 CFR 274.6 - Division/district pest control programs.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 33 Navigation and Navigable Waters 3 2014-07-01 2014-07-01 false Division/district pest control..., DEPARTMENT OF DEFENSE PEST CONTROL PROGRAM FOR CIVIL WORKS PROJECTS Project Operation § 274.6 Division/district pest control programs. (a) Guides. Referenced technical manuals, and Engineer Circulars issued...

33 CFR 274.6 - Division/district pest control programs.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 33 Navigation and Navigable Waters 3 2011-07-01 2011-07-01 false Division/district pest control..., DEPARTMENT OF DEFENSE PEST CONTROL PROGRAM FOR CIVIL WORKS PROJECTS Project Operation § 274.6 Division/district pest control programs. (a) Guides. Referenced technical manuals, and Engineer Circulars issued...

Publications - SR 47 | Alaska Division of Geological & Geophysical Surveys

Science.gov Websites

and Facilities Staff Seismic and Well Data Data Reports Contact Us Frequently Asked Questions Ask a , T.K., Clough, A.H., Hansen, E.W., and Nelson, M.G. Publication Date: 1993 Publisher: Alaska Division ., Clough, A.H., Hansen, E.W., and Nelson, M.G., 1993, Alaska's mineral industry 1992: Alaska Division of

Publications - GMC 397 | Alaska Division of Geological & Geophysical

Science.gov Websites

: Apache Corp., Alaska Division of Oil and Gas, and Weatherford Laboratories Publication Date: Nov 2011 Apache Corp., Alaska Division of Oil and Gas, and Weatherford Laboratories, 2011, Porosity and Files gmc397.pdf (2.8 M) gmc397.zip (24.2 M) Keywords Cook Inlet Basin; Oil and Gas; Permeability

75 FR 77664 - Honeywell International, Inc., Automation and Control Solutions Division, Including On-Site...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-13

..., Inc., Automation and Control Solutions Division, Including On-Site Leased Workers From Manpower...., Automation and Control Solutions Division, Rock Island, Illinois. The notice was published in the Federal...-site at the Rock Island, Illinois location of Honeywell International, Inc., Automation and Control...

Deregulation of cell growth and malignant transformation.

PubMed

Sulić, Sanda; Panić, Linda; Dikić, Ivan; Volarević, Sinisa

2005-08-01

Cell growth and cell division are fundamental aspects of cell behavior in all organisms. Recent insights from many model organisms have shed light on the molecular mechanisms that control cell growth and cell division. A significant body of evidence has now been accumulated, showing a direct link between deregulation of components of cell cycle machinery and cancer. In addition, defects in one or more steps that control growth are important for malignant transformation, as many tumor suppressors and proto-oncogenes have been found to regulate cell growth. The importance of cell growth in tumor development is further supported by the discovery that rapamycin, an effective anticancer drug, inhibits a key regulator of protein synthetic machinery and cell growth, mammalian target of rapamycin (mTOR). In most cases, cell growth and cell division are coupled, thereby maintaining cell size within physiological limits. We believe that, in a long-term perspective, understanding how these two processes are coordinated in vivo and how their interplay is deregulated in a number of diseases, including cancer, may have a direct impact on the efficiency of modern therapeutics.

Stewart Air National Guard Base, NY, C-5M Painting Refurbishment Assessment

DTIC Science & Technology

2012-12-06

is asthma due to sensitization. After sensitization, any exposure , even to levels below the occupational exposure limit, can produce an asthmatic...Consultative Services Division provide a comprehensive exposure and risk assessment of the corrosion control process conducted on the C-5M in Building...101 at Stewart Air National Guard Base, NY. This facility was previously a fuels systems maintenance facility. At the time of this assessment, the

Test of Magnetic Rotation near the band head in ^197,198Pb

NASA Astrophysics Data System (ADS)

Krücken, R.; Clark, R. M.; Deleplanque, M. A.; Diamond, R. M.; Fallon, P.; Macchiavelli, A. O.; Lee, I. Y.; Schmid, G. J.; Stephens, F. S.; Vetter, K.; Dewald, A.; Peusquens, R.; von Brentano, P.; Baldsiefen, G.; Chmel, S.; Hübel, H.; Becker, J. A.; Bernstein, L. A.; Hauschild, K.

1998-04-01

The concept of magnetic rotation is tested near the band head of shears-bands in ^197,198Pb by means of a lifetime experiment with the recoil distance method (RDM). The experiment was performed using the Gammasphere array in conjunction with the Cologne Plunger. The B(M1) values extracted from the measured lifetimes can prove the applicability of the concept of magnetic rotation for the states near the band head of these shears bands. The RDM results are compared with tilted axis cranking and shell model calculations. Furthermore the results will be used to test earlier DSAM lifetime measurements for states at higher spins. Preliminary results of this topic will be presented. This work is supported by DOE grant numbers DE-AC03-76SF00098 (LBNL), DE-FG02-91ER40609 (Yale), W-7405-ENG-48 (LLNL) and by the German BMBF for Cologne (No. 06 OK 668) and Bonn.

Laser plasma coupling with moderate Z, long scalelength underdense plasma

NASA Astrophysics Data System (ADS)

Kruer, William; Berger, Richard; Meezan, Nathaniel; Suter, Larry; Moody, John; Glenzer, Siegfried; Stevenson, R. M.; Oades, K.

2004-11-01

Recent experiments1,2 have focussed new attention on the coupling of laser light with moderate Z, long scalelength underdense plasmas. We discuss some intriguing features of these experiments, including a significant reduction of stimulated Raman and Brillouin scattering in higher Z plasmas, such as Krypton and Xenon. Threshold conditions for various instabilities are discussed, and potential consequences of thermal filamentation and self-focussing are explored. The presence of significant temperature modulations in the plasma can lead to a number of interesting effects not usually taken into account, such as ion wave refraction out of hot spots and instability reduction by the long wavelength modulations. We also consider the extrapolation of these results to the higher temperature regimes more relevant to ignition-scale hohlraums. 1. R. M. Stevenson, et. al, Phys. Plasmas 11, 2709 (2004) 2. J. Moody (to be published) Work performed under the auspices of the U.S. DOE by the Lawrence Livermore National Laboratory under Contract W-7405-ENG-48.

40 CFR 52.2320 - Identification of plan.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Regulations R446-1-1 and R446-1-4.9 to statutory requirements for application of reasonably available control... Metropolitan Statistical Areas as required by section 211(m) of the Clean Air Act Amendments of 1990. (i..., 1996 (with minor mathematical corrections submitted by the Utah Division of Air Quality on August 12...

77 FR 40354 - Permitting Guidance for Oil and Gas Hydraulic Fracturing Activities Using Diesel Fuels-Draft

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-09

... INFORMATION CONTACT: Sherri Comerford, Underground Injection Control (UIC) Program, Drinking Water Protection Division, Office of Ground Water and Drinking Water (MC-4606M), Environmental Protection Agency, 1200... Fracturing and the Safe Drinking Water Act Web site, http://water.epa.gov/type/groundwater/uic/class2...

Waste minimization/pollution prevention study of high-priority waste streams

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ogle, R.B.

1994-03-01

Although waste minimization has been practiced by the Metals and Ceramics (M&C) Division in the past, the effort has not been uniform or formalized. To establish the groundwork for continuous improvement, the Division Director initiated a more formalized waste minimization and pollution prevention program. Formalization of the division`s pollution prevention efforts in fiscal year (FY) 1993 was initiated by a more concerted effort to determine the status of waste generation from division activities. The goal for this effort was to reduce or minimize the wastes identified as having the greatest impact on human health, the environment, and costs. Two broadmore » categories of division wastes were identified as solid/liquid wastes and those relating to energy use (primarily electricity and steam). This report presents information on the nonradioactive solid and liquid wastes generated by division activities. More specifically, the information presented was generated by teams of M&C staff members empowered by the Division Director to study specific waste streams.« less

235U Holdup Measurements in the 321-M Exhaust Elbows

DOE Office of Scientific and Technical Information (OSTI.GOV)

Salaymeh, S.R.

The Analytical Development Section of Savannah River Technology Center (SRTC) was requested by the Facilities Disposition Division (FDD) to determine the holdup of enriched uranium in the 321-M facility as part of an overall deactivation project of the facility. The 321-M facility was used to fabricate enriched uranium fuel assemblies, lithium-aluminum target tubes, neptunium assemblies, and miscellaneous components for the production reactors. The facility also includes the 324-M storage building and the passageway connecting it to 321-M. The results of the holdup assays are essential for determining compliance with the Waste Acceptance Criteria, Material Control and Accountability, and to meetmore » criticality safety controls. This report covers holdup measurements of uranium residue in the exhaust piping elbows removed from the roof the 321-M facility.« less

Division rate, cell size and proteome allocation: impact on gene expression noise and implications for the dynamics of genetic circuits

PubMed Central

2018-01-01

The cell division rate, size and gene expression programmes change in response to external conditions. These global changes impact on average concentrations of biomolecule and their variability or noise. Gene expression is inherently stochastic, and noise levels of individual proteins depend on synthesis and degradation rates as well as on cell-cycle dynamics. We have modelled stochastic gene expression inside growing and dividing cells to study the effect of division rates on noise in mRNA and protein expression. We use assumptions and parameters relevant to Escherichia coli, for which abundant quantitative data are available. We find that coupling of transcription, but not translation rates to the rate of cell division can result in protein concentration and noise homeostasis across conditions. Interestingly, we find that the increased cell size at fast division rates, observed in E. coli and other unicellular organisms, buffers noise levels even for proteins with decreased expression at faster growth. We then investigate the functional importance of these regulations using gene regulatory networks that exhibit bi-stability and oscillations. We find that network topology affects robustness to changes in division rate in complex and unexpected ways. In particular, a simple model of persistence, based on global physiological feedback, predicts increased proportion of persister cells at slow division rates. Altogether, our study reveals how cell size regulation in response to cell division rate could help controlling gene expression noise. It also highlights that understanding circuits' robustness across growth conditions is key for the effective design of synthetic biological systems. PMID:29657814

M31 Globular Clusters and Galaxy Formation

NASA Astrophysics Data System (ADS)

Gregg, M. D.; Karick, A. M.

2005-12-01

The brightest globular cluster in the halo of M31, cluster G1, has properties which suggest that it is not an ordinary globular but an ultra-compact dwarf galaxy: its velocity dispersion, M/L, and ellipticity are all atypically large, and its color-magnitude diagram suggests an abundance spread. Using the Keck Laser Guide Star Adaptive Optics system with NIRC2, we have begun an imaging campaign of globular clusters in M31 to measure their core sizes. Combining these data with high dispersion spectroscopy will produce masses and M/L ratios to determine if there are additional UCDs masquerading as M31 globulars. UCDs are thought to be the remnant nuclei from tidally stripped dwarf ellipticals or small spirals; finding additional examples in the cluster system of M31 has implications for galaxy formation processes. The K-band image quality during our first LGS run was very stable over many hours, with Strehl ratios of 0.35 or better, producing point sources with FWHM of 0\\farcs05. The core sizes of the clusters, which range from 0\\farcs2 to 0\\farcs8 can be easily measured from these data. The observing conditions were nearly as good in the J-band, and we obtained both colors for a number of clusters. We discuss our efforts to produce photometrically-calibrated color-magnitude diagrams of the clusters. This work is supported by National Science Foundation Grant No. 0407445 and was done at the Institute of Geophysics and Planetary Physics, under the auspices of the U.S. Department of Energy by Lawrence Livermore National Laboratory under contract No. W-7405-Eng-48.

The origins of thalamic inputs to grasp zones in frontal cortex of macaque monkeys

PubMed Central

Stepniewska, Iwona; Kaas, Jon H.

2015-01-01

The hand representation in primary motor cortex (M1) is instrumental to manual dexterity in primates. In Old World monkeys, rostral and caudal aspects of the hand representation are located in the precentral gyrus and the anterior bank of the central sulcus, respectively. We previously reported the organization of the cortico-cortical connections of the grasp zone in rostral M1. Here we describe the organization of thalamocortical connections that were labeled from the same tracer injections. Thalamocortical connections of a grasp zone in ventral premotor cortex (PMv) and the M1 orofacial representation are included for direct comparison. The M1 grasp zone was primarily connected with ventral lateral divisions of motor thalamus. The largest proportion of inputs originated in the posterior division (VLp) followed by the medial and the anterior divisions. Thalamic inputs to the M1 grasp zone originated in more lateral aspects of VLp as compared to the origins of thalamic inputs to the M1 orofacial representation. Inputs to M1 from thalamic divisions connected with cerebellum constituted three fold the density of inputs from divisions connected with basal ganglia, whereas the ratio of inputs was more balanced for the grasp zone in PMv. Privileged access of the cerebellothalamic pathway to the grasp zone in rostral M1 is consistent with the connection patterns previously reported for the precentral gyrus. Thus, cerebellar nuclei are likely more involved than basal ganglia nuclei with the contributions of rostral M1 to manual dexterity. PMID:26254903

The origins of thalamic inputs to grasp zones in frontal cortex of macaque monkeys.

PubMed

Gharbawie, Omar A; Stepniewska, Iwona; Kaas, Jon H

2016-07-01

The hand representation in primary motor cortex (M1) is instrumental to manual dexterity in primates. In Old World monkeys, rostral and caudal aspects of the hand representation are located in the precentral gyrus and the anterior bank of the central sulcus, respectively. We previously reported the organization of the cortico-cortical connections of the grasp zone in rostral M1. Here we describe the organization of thalamocortical connections that were labeled from the same tracer injections. Thalamocortical connections of a grasp zone in ventral premotor cortex (PMv) and the M1 orofacial representation are included for direct comparison. The M1 grasp zone was primarily connected with ventral lateral divisions of motor thalamus. The largest proportion of inputs originated in the posterior division (VLp) followed by the medial and the anterior divisions. Thalamic inputs to the M1 grasp zone originated in more lateral aspects of VLp as compared to the origins of thalamic inputs to the M1 orofacial representation. Inputs to M1 from thalamic divisions connected with cerebellum constituted three fold the density of inputs from divisions connected with basal ganglia, whereas the ratio of inputs was more balanced for the grasp zone in PMv. Privileged access of the cerebellothalamic pathway to the grasp zone in rostral M1 is consistent with the connection patterns previously reported for the precentral gyrus. Thus, cerebellar nuclei are likely more involved than basal ganglia nuclei with the contributions of rostral M1 to manual dexterity.

Accumulation of neutral mutations in growing cell colonies with competition.

PubMed

Sorace, Ron; Komarova, Natalia L

2012-12-07

Neutral mutations play an important role in many biological processes including cancer initiation and progression, the generation of drug resistance in bacterial and viral diseases as well as cancers, and the development of organs in multicellular organisms. In this paper we study how neutral mutants are accumulated in nonlinearly growing colonies of cells subject to growth constraints such as crowding or lack of resources. We investigate different types of growth control which range from "division-controlled" to "death-controlled" growth (and various mixtures of both). In division-controlled growth, the burden of handling overcrowding lies with the process of cell-divisions, the divisions slow down as the carrying capacity is approached. In death-controlled growth, it is death rate that increases to slow down expansion. We show that division-controlled growth minimizes the number of accumulated mutations, and death-controlled growth corresponds to the maximum number of mutants. We check that these results hold in both deterministic and stochastic settings. We further develop a general (deterministic) theory of neutral mutations and achieve an analytical understanding of the mutant accumulation in colonies of a given size in the absence of back-mutations. The long-term dynamics of mutants in the presence of back-mutations is also addressed. In particular, with equal forward- and back-mutation rates, if division-controlled and a death-controlled types are competing for space and nutrients, cells obeying division-controlled growth will dominate the population. Copyright © 2012 Elsevier Ltd. All rights reserved.

Effects of dimethyl sulfoxide on asymmetric division and cytokinesis in mouse oocytes.

PubMed

Zhou, Dongjie; Shen, Xinghui; Gu, Yanli; Zhang, Na; Li, Tong; Wu, Xi; Lei, Lei

2014-06-21

Dimethyl sulfoxide (DMSO) is used extensively as a permeable cryoprotectant and is a common solvent utilized for several water-insoluble substances. DMSO has various biological and pharmacological activities; however, the effect of DMSO on mouse oocyte meiotic maturation remains unknown. In DMSO-treated oocytes, we observed abnormal MII oocytes that contained large polar bodies, including 2-cell-like MII oocytes, during in vitro maturation. Oocyte polarization did not occur, due to the absence of actin cap formation and spindle migration. These features are among the primary causes of abnormal symmetric division; however, analysis of the mRNA expression levels of genes related to asymmetric division revealed no significant difference in the expression of these factors between the 3% DMSO-treated group and the control group. After each "blastomere" of the 2-cell-like MII stage oocytes was injected by one sperm head respectively, the oocytes still possessed the ability to extrude the second polar body from each "blastomere" and to begin cleavage. However, MII oocytes with large polar bodies developed to the blastocyst stage after intracytoplasmic sperm injection (ICSI). Furthermore, other permeable cryoprotectants, such as ethylene glycol and glycerol, also caused asymmetric division failure. Permeable cryoprotectants, such as DMSO, ethylene glycol, and glycerol, affect asymmetric division. DMSO disrupts cytokinesis completion by inhibiting cortical reorganization and polarization. Oocytes that undergo symmetric division maintain the ability to begin cleavage after ICSI.

Instrumentation and Controls Division progress report, July 1, 1990--June 30, 1992. Volume 2

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1993-01-01

This report contains the following information from the Instrumentation and Controls Division of Oak Ridge National Laboratory: supplementary activities; seminars; publications and presentations; scientific and professional activities, achievements, and awards; and division organization charts.

Multi-modal virtual environment research at Armstrong Laboratory

NASA Technical Reports Server (NTRS)

Eggleston, Robert G.

1995-01-01

One mission of the Paul M. Fitts Human Engineering Division of Armstrong Laboratory is to improve the user interface for complex systems through user-centered exploratory development and research activities. In support of this goal, many current projects attempt to advance and exploit user-interface concepts made possible by virtual reality (VR) technologies. Virtual environments may be used as a general purpose interface medium, an alternative display/control method, a data visualization and analysis tool, or a graphically based performance assessment tool. An overview is given of research projects within the division on prototype interface hardware/software development, integrated interface concept development, interface design and evaluation tool development, and user and mission performance evaluation tool development.

U-235 Holdup Measurements in the 321-M Lathe HEPA Banks

DOE Office of Scientific and Technical Information (OSTI.GOV)

Salaymeh, S.R.

The Analytical Development Section of Savannah River Technology Center (SRTC) was requested by the Facilities Decommissioning Division (FDD) to determine the holdup of enriched uranium in the 321-M facility as part of an overall deactivation project of the facility. The results of the holdup assays are essential for determining compliance with the Waste Acceptance Criteria, Material Control and Accountability, and to meet criticality safety controls. This report covers holdup measurements of uranium residue in six high efficiency particulate air (HEPA) filter banks of the A-lathe and B-lathe exhaust systems of the 321-M facility. This report discusses the non-destructive assay measurements,more » assumptions, calculations, and results of the uranium holdup in these six items.« less

Polyamidoamine (PAMAM) dendrimers as potential release modulators and oral bioavailability enhancers of vardenafil hydrochloride.

PubMed

Tawfik, Mai Ahmed; Tadros, Mina Ibrahim; Mohamed, Magdy Ibrahim

2018-05-21

Vardenafil hydrochloride (VAR) is an erectile dysfunction treating drug. VAR has a short elimination half-life (4-5 h) and suffers low oral bioavailability (15%). This work aimed to explore the dual potential of VAR-dendrimer complexes as drug release modulators and oral bioavailability enhancers. VAR-dendrimer complexes were prepared by solvent evaporation technique using four dendrimer generations (G4.5, G5, G5.5 and G6) at three concentrations (190 nM, 380 nM and 950 nM). The systems were evaluated for intermolecular interactions, particle size, zeta potential, drug entrapment efficiency percentages (EE%) and drug released percentages after 2 h (Q 2h ) and 24 h (Q 24h ). The results were statistically analyzed, and the system showing the highest desirability was selected for further pharmacokinetic studies in rabbits, in comparison to Levitra ® tablets. The highest desirability (0.82) was achieved with D10 system comprising VAR (10 mg) and G6 (190 nM). It possessed small particle size (113.85 nm), low PDI (0.19), positive zeta potential (+21.53), high EE% (75.24%), promising Q 2 h (41.45%) and Q 24 h (74.05%). Compared to Levitra ® tablets, the significantly (p < 0.01) delayed T max , prolonged MRT (0-∞) and higher relative bioavailability (3.7-fold) could clarify the dual potential of D10 as a sustained release system capable of enhancing VAR oral bioavailability.

Architecture and inherent robustness of a bacterial cell-cycle control system.

PubMed

Shen, Xiling; Collier, Justine; Dill, David; Shapiro, Lucy; Horowitz, Mark; McAdams, Harley H

2008-08-12

A closed-loop control system drives progression of the coupled stalked and swarmer cell cycles of the bacterium Caulobacter crescentus in a near-mechanical step-like fashion. The cell-cycle control has a cyclical genetic circuit composed of four regulatory proteins with tight coupling to processive chromosome replication and cell division subsystems. We report a hybrid simulation of the coupled cell-cycle control system, including asymmetric cell division and responses to external starvation signals, that replicates mRNA and protein concentration patterns and is consistent with observed mutant phenotypes. An asynchronous sequential digital circuit model equivalent to the validated simulation model was created. Formal model-checking analysis of the digital circuit showed that the cell-cycle control is robust to intrinsic stochastic variations in reaction rates and nutrient supply, and that it reliably stops and restarts to accommodate nutrient starvation. Model checking also showed that mechanisms involving methylation-state changes in regulatory promoter regions during DNA replication increase the robustness of the cell-cycle control. The hybrid cell-cycle simulation implementation is inherently extensible and provides a promising approach for development of whole-cell behavioral models that can replicate the observed functionality of the cell and its responses to changing environmental conditions.

TRADOC Annual Command History, 1 January to 31 December 1991

DTIC Science & Technology

1992-06-01

24th Infantry Division (Mechanized), the French 6th Light Armored Division, the 3d Armored Cavalry Regiment, and other units assigned; and VII Corps...under Lt. Gen. Frederick M. Franks, Jr., with the U.S. Ist Infantry Division (Mechanized), Ist and 3d Armored Divisions, the Ist Cavalry Division, the...Alma Ata, Kazakhstan, the presidents of all the former republics except Georgia and the three seceding Baltic states, declared formation of the

Application of close-packed structures in dental resin composites.

PubMed

Wang, Ruili; Habib, Eric; Zhu, X X

2017-03-01

The inorganic filler particles in dental resin composites serve to improve their mechanical properties and reduce polymerization shrinkage during their use. Efforts have been made in academia and industry to increase the filler particle content, but, few studies examine the theoretical basis for the maximum particle loading. This work evaluates the packing of spherical particles in a close-packed state for highly loaded composites. Calculations show that for low dispersity particles, the maximum amount of particles is 74.05vol%, regardless of the particle size. This can be further improved by using a mix of large and small particles or by the use of non-spherical particles. For representative spherical particles with a diameter of 1000nm, two types of secondary particles with respective sizes of 414nm (d I ) and 225nm (d II ) are selected. The results show that after embedding secondary particles I & II into primary spherical particles, the packing factor is increased to 81.19% for the close-packed structures, which shows an improvement of 9.64%, compared to the 74.05% obtained only with primary spherical particles. This packing factor is also higher than either structure with the embedded secondary particles I or II. Examples of these mixtures with different spherical particle sizes are shown as a theoretical estimation, serving as a guideline for the design and formulation of new dental resin composites with better properties and improved performance. Copyright © 2017 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

Effects of dimethyl sulfoxide on asymmetric division and cytokinesis in mouse oocytes

PubMed Central

2014-01-01

Background Dimethyl sulfoxide (DMSO) is used extensively as a permeable cryoprotectant and is a common solvent utilized for several water-insoluble substances. DMSO has various biological and pharmacological activities; however, the effect of DMSO on mouse oocyte meiotic maturation remains unknown. Results In DMSO-treated oocytes, we observed abnormal MII oocytes that contained large polar bodies, including 2-cell–like MII oocytes, during in vitro maturation. Oocyte polarization did not occur, due to the absence of actin cap formation and spindle migration. These features are among the primary causes of abnormal symmetric division; however, analysis of the mRNA expression levels of genes related to asymmetric division revealed no significant difference in the expression of these factors between the 3% DMSO-treated group and the control group. After each “blastomere” of the 2-cell–like MII stage oocytes was injected by one sperm head respectively, the oocytes still possessed the ability to extrude the second polar body from each “blastomere” and to begin cleavage. However, MII oocytes with large polar bodies developed to the blastocyst stage after intracytoplasmic sperm injection (ICSI). Furthermore, other permeable cryoprotectants, such as ethylene glycol and glycerol, also caused asymmetric division failure. Conclusion Permeable cryoprotectants, such as DMSO, ethylene glycol, and glycerol, affect asymmetric division. DMSO disrupts cytokinesis completion by inhibiting cortical reorganization and polarization. Oocytes that undergo symmetric division maintain the ability to begin cleavage after ICSI. PMID:24953160

Changes in period mRNA levels in the brain and division of labor in honey bee colonies

PubMed Central

Toma, Dan P.; Bloch, Guy; Moore, Darrell; Robinson, Gene E.

2000-01-01

Previous research showed that age-related division of labor in honey bees is associated with changes in activity rhythms; young adult bees perform hive tasks with no daily rhythms, whereas older bees forage with strong daily rhythms. We report that this division of labor is also associated with differences in both circadian rhythms and mRNA levels of period, a gene well known for its role in circadian rhythms. The level of period mRNA in the brain oscillated in bees of all ages, but was significantly higher at all times in foragers. Elevated period mRNA levels cannot be attributed exclusively to aging, because bees induced to forage precociously because of a change in social environment had levels similar to normal age foragers. These results extend the regulation of a “clock gene” to a social context and suggest that there are connections at the molecular level between division of labor and chronobiology in social insects. PMID:10841583

Collaborative Information Technologies

NASA Astrophysics Data System (ADS)

Meyer, William; Casper, Thomas

1999-11-01

Significant effort has been expended to provide infrastructure and to facilitate the remote collaborations within the fusion community and out. Through the Office of Fusion Energy Science Information Technology Initiative, communication technologies utilized by the fusion community are being improved. The initial thrust of the initiative has been collaborative seminars and meetings. Under the initiative 23 sites, both laboratory and university, were provided with hardware required to remotely view, or project, documents being presented. The hardware is capable of delivering documents to a web browser, or to compatible hardware, over ESNET in an access controlled manner. The ability also exists for documents to originate from virtually any of the collaborating sites. In addition, RealNetwork servers are being tested to provide audio and/or video, in a non-interactive environment with MBONE providing two-way interaction where needed. Additional effort is directed at remote distributed computing, file systems, security, and standard data storage and retrieval methods. This work supported by DoE contract No. W-7405-ENG-48

Investigating Autonomic Control of the Cardiovascular System: A Battery of Simple Tests

ERIC Educational Resources Information Center

Johnson, Christopher D.; Roe, Sean; Tansey, Etain A.

2013-01-01

Sympathetic and parasympathetic divisions of the autonomic nervous system constantly control the heart (sympathetic and parasympathetic divisions) and blood vessels (predominantly the sympathetic division) to maintain appropriate blood pressure and organ blood flow over sometimes widely varying conditions. This can be adversely affected by…

Measurement of the 1s Hyperfine Transition of Two Tl^80+ Isotopes

NASA Astrophysics Data System (ADS)

Beiersdorfer, P.; Utter, S. B.; Wong, K. L.; Crespo López-Urrutia, J. R.; Britten, J. A.; Chen, H.; Thoe, R. S.; Thorn, D. B.; Träbert, E.; Gustavsson, M. G. H.; Forssén, C.; Mårtenson-Pendrill, A.-M.; Harris, C. L.

2001-05-01

The hyperfine splitting of the 1s ground state has been measured for the two stable isotopes of hydrogen-like Tl using emission spectroscopy in the SuperEBIT electron beam ion trap. The results are 3858.22± 0.30 Åfor ^203Tl^80+ and 3821.84± 0.34 Åfor ^205Tl^80+. These differ by about 60 Å from recent and about 19 Å from very recent calculations, illustrating unsolved issues affecting these transitions in hydrogen-like ions. The wavelength difference Δλ = 36.38± 0.35 Å is consistent with estimates based on hyperfine anomaly data for neutral Tl. By using previously determined nuclear magnetic moments and applying appropriate corrections for the nuclear charge distribution and radiative effects, the experimental splittings can be interpreted in terms of nuclear magnetization radii < r^2_m>^1/2= 5.83(14) fm for ^203Tl and < r^2_m>^1/2= 5.89(14) fm for ^205Tl. These values are 10% larger than derived from single-particle nuclear magnetization models, and are slightly larger than the corresponding charge distributions. *Work performed under the auspices of DOE by UCLLNL under contract W-7405-ENG-48 and supported by the Office of Basic Energy Sciences.

Site environmental report for Calendar Year 1994 on radiological and nonradiological parameters

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1995-06-30

Battelle Memorial Institute`s nuclear research facilities are currently being maintained in a surveillance and maintenance (S&M) mode with continual decontamination and decommissioning (D&D) activities being conducted under Department of Energy (DOE) Contract W-7405-ENG-92. These activities are referred to under the Contract as the Battelle Columbus Laboratories Decommissioning Project (BCLDP). Operations referenced in this report are performed in support of S&M and D&D activities. Battelle`s King Avenue facility is not considered in this report to the extent that the West Jefferson facility is. The source term at the King Avenue site is a small fraction of the source term at themore » West Jefferson site. Off site levels of radionuclides that could be attributed to the west Jefferson and King Avenue nuclear operations wereindistinguishable from background levels at specific locations where air, water, and direct radiation measurements were performed. Environmental monitoring continued to demonstrate compliance by Battelle with federal, state and local regulations. Routine, nonradiological activities performed include monitoring liquid effluents and monitoring the ground water system for the West Jefferson North site. Samples of various environmental media including air, water, grass, fish, field and garden crops, sediment and soil were collected from the region surrounding the two sites and analyzed.« less

77 FR 12318 - National Cancer Institute; Notice of Closed Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-29

... Research in Integrative Cancer Biology and the Tumor Microenvironment. Date: March 20, 2012. Time: 8 a.m... Review Officer, Special Review & Logistics Branch, Division of Extramural Activities, National Cancer...: Ilda M McKenna, Ph.D., Scientific Review Officer, Research Training Review Branch, Division of...

Deregulation of HEF1 Impairs M-Phase Progression by Disrupting the RhoA Activation Cycle

PubMed Central

Dadke, Disha; Jarnik, Michael; Pugacheva, Elena N.; Singh, Mahendra K.; Golemis, Erica A.

2006-01-01

The focal adhesion-associated signaling protein HEF1 undergoes a striking relocalization to the spindle at mitosis, but a function for HEF1 in mitotic signaling has not been demonstrated. We here report that overexpression of HEF1 leads to failure of cells to progress through cytokinesis, whereas depletion of HEF1 by small interfering RNA (siRNA) leads to defects earlier in M phase before cleavage furrow formation. These defects can be explained mechanistically by our determination that HEF1 regulates the activation cycle of RhoA. Inactivation of RhoA has long been known to be required for cytokinesis, whereas it has recently been determined that activation of RhoA at the entry to M phase is required for cellular rounding. We find that increased HEF1 sustains RhoA activation, whereas depleted HEF1 by siRNA reduces RhoA activation. Furthermore, we demonstrate that chemical inhibition of RhoA is sufficient to reverse HEF1-dependent cellular arrest at cytokinesis. Finally, we demonstrate that HEF1 associates with the RhoA-GTP exchange factor ECT2, an orthologue of the Drosophila cytokinetic regulator Pebble, providing a direct means for HEF1 control of RhoA. We conclude that HEF1 is a novel component of the cell division control machinery and that HEF1 activity impacts division as well as cell attachment signaling events. PMID:16394104

Division 1137 property control system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pastor, D.J.

1982-01-01

An automated data processing property control system was developed by Mobile and Remote Range Division 1137. This report describes the operation of the system and examines ways of using it in operational planning and control.

NRL Fact Book

DTIC Science & Technology

1983-03-01

Mr. W.S. Kratz BMC M.R. Fishe Mr. R.G. Trott Administrative Officer Security Officer r Operations Officer Facilities Manager and Head, Maintenance and...Division 20 Financial Management Division 22 Management Information Division 24 Civilian Personnel Division 26 THE TECHNICAL SERVICES DIRECTORATE 29 Office...the neighborhood of 3200. The overall management of the Laboratory is under the direction of a Naval Commanding Officer -nd a civilian Director of

Shear Moduli of Orthotropic Composites.

DTIC Science & Technology

1980-03-01

COMPOSITES N. J. Pagano Mechanics & Surface Interactions Branch Nonmetallic Materials Division March 1980 DT1C> Technical Report AFML-TR-79-4164 ELE(I JUN...Surface Interactions Br. Nonmetallic Materials Division Nonmetallic Materials Division FOR THE COMMANDER M.KLLChief metallic Materials Division "If your...SrtaceT’ Ileation ranch 10. PROGRAM ELEMENT. PROJECT, TASK Materials Laboratory 219 u,\\i’- Air Force Wright Aeronautical Laboratories 21310, WD Wright

40 CFR 52.1977 - Content of approved State submitted implementation plan.

Code of Federal Regulations, 2013 CFR

2013-07-01

...) Division 28—Stationary Source Air Pollution Control and Permitting Procedures Excess Emissions and... Requirements (11/4/93) 28-1450Enforcement Action Criteria (9/24/93) Division 200—General Air Pollution... Designations (10/14/99) 204-0090Oxygenated Gasoline Control Areas (3/27/01) Division 206—Air Pollution...

40 CFR 52.1977 - Content of approved State submitted implementation plan.

Code of Federal Regulations, 2012 CFR

2012-07-01

...) Division 28—Stationary Source Air Pollution Control and Permitting Procedures Excess Emissions and... Requirements (11/4/93) 28-1450Enforcement Action Criteria (9/24/93) Division 200—General Air Pollution... Designations (10/14/99) 204-0090Oxygenated Gasoline Control Areas (3/27/01) Division 206—Air Pollution...

40 CFR 52.1977 - Content of approved State submitted implementation plan.

Code of Federal Regulations, 2014 CFR

2014-07-01

...) Division 28—Stationary Source Air Pollution Control and Permitting Procedures Excess Emissions and... Requirements (11/4/93) 28-1450Enforcement Action Criteria (9/24/93) Division 200—General Air Pollution... Designations (10/14/99) 204-0090Oxygenated Gasoline Control Areas (3/27/01) Division 206—Air Pollution...

40 CFR 52.1977 - Content of approved State submitted implementation plan.

Code of Federal Regulations, 2011 CFR

2011-07-01

...) Division 28—Stationary Source Air Pollution Control and Permitting Procedures Excess Emissions and... Requirements (11/4/93) 28-1450Enforcement Action Criteria (9/24/93) Division 200—General Air Pollution... Designations (10/14/99) 204-0090Oxygenated Gasoline Control Areas (3/27/01) Division 206—Air Pollution...

75 FR 41551 - Meetings of Humanities Panel

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-16

... Psychology in Fellowships, submitted to the Division of Research Programs at the May 4, 2010 deadline. 21... applications for Advanced Social Science Research on Japan in Fellowships, submitted to the Division of...:30 a.m. to 5 p.m. Room: 415. Program: This meeting will review applications for Social Sciences and...

78 FR 50065 - National Cancer Institute; Notice of Closed Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-16

... Methodological Research for Cancer Epidemiology Cohorts. Date: October 25, 2013. Time: 10:30 a.m. to 2:30 p.m... Lopaczynski, MD, Ph.D., Scientific Review Officer, Research Programs Review Branch, Division of Extramural.... Contact Person: Shakeel Ahmad, Ph.D., Scientific Review Officer, Research Programs Review Branch, Division...

76 FR 12996 - Proposal Review Panel for Chemistry; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-09

... NATIONAL SCIENCE FOUNDATION Proposal Review Panel for Chemistry; Notice of Meeting In accordance... Awardees by NSF Division of Chemistry (CHE), 1191. Dates and Times: March 31, 2011; 8 a.m.-5:30 p.m. April... Director, Chemistry Centers Program, Division of Chemistry, Room 1055, National Science Foundation, 4201...

76 FR 6499 - Proposal Review Panel for Chemistry; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-04

... NATIONAL SCIENCE FOUNDATION Proposal Review Panel for Chemistry; Notice of Meeting In accordance... Awardees by NSF Division of Chemistry (1191). Dates and Times: February 17, 2011; 8 a.m.-6 p.m. February 18... Director, Chemistry Centers Program, Division of Chemistry, Room 1055, National Science Foundation, 4201...

75 FR 39546 - National Cancer Institute; Notice of Closed Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-09

...; Integrative Cancer Biology in the Tumor Microenvironment (U01). Date: July 22, 2010. Time: 8 a.m. to 5 p.m..., Special Review and Logistics Branch, Division of Extramural Activities, National Cancer Institute, 6116... Review and Logistic Branch, Division of Extramural Activities, NCI, National Institutes of Health, 6116...

78 FR 8155 - National Cancer Institute; Notice of Closed Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-05

...; NCI Omnibus Cancer Biology 1. Date: March 11-12, 2013. Time: 8:00 a.m. to 5:00 p.m. Agenda: To review..., Special Review and Logistics Branch, Division of Extramural Activities, National Cancer Institute, NIH..., MD, Scientific Review Officer, Research Programs Review Branch, Division of Extramural Activities...

75 FR 21640 - National Cancer Institute; Notice of Closed Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-26

..., Resources and Training Review Branch, Division of Extramural Activities, National Cancer Institute, 6116....D., PhD, Scientific Review Officer, Research Programs Review Branch, Division of Extramural... & Tissue Biology P01. Date: May 26-28, 2010. Time: 5 p.m. to 5 p.m. Agenda: To review and evaluate grant...

76 FR 14675 - National Cancer Institute; Notice of Closed Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-17

... Biology. Date: May 16-18, 2011. Time: 4 p.m. to 5 p.m. Agenda: To review and evaluate grant applications... Branch, Division of Extramural Activities, National Cancer Institute, NIH, 6116 Executive Boulevard, Room.... Wirth, PhD, Scientific Review Officer, Research Programs Review Branch, Division of Extramural...

78 FR 26379 - National Cancer Institute; Notice of Closed Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-06

... Methodological Research for Cancer Epidemiology Cohorts. Date: June 25, 2013. Time: 11:00 a.m. to 4:00 p.m... Officer, Research Programs Review Branch, Division of Extramural Activities, National Cancer Institute... Person: David G. Ransom, Ph.D., Scientific Review Officer, Research Programs Review Branch, Division of...

Physical qualities and activity profiles of sub-elite and recreational Australian football players.

PubMed

Stein, Josh G; Gabbett, Tim J; Townshend, Andrew D; Dawson, Brian T

2015-11-01

To investigate the relationship between physical qualities and match activity profiles of recreational Australian football players. Prospective cohort study. Forty players from three recreational Australian football teams (Division One, Two and Three) underwent a battery of fitness tests (vertical jump, 10 and 40 m sprint, 6 m × 30 m repeated sprint test, Yo-Yo intermittent recovery level Two and 2-km time trial). The activity profiles of competitive match-play were quantified using 10-Hz Global Positioning System units. Division One players possessed greater maximum velocity, Yo-Yo level Two and 2-km time trial performances than Division Two and Three players. In addition, Division One players covered greater relative distance, and relative distances at moderate- and high-intensities during match-play than Division Two and Three players. Division Two players had better 2-km time trial performances than Division Three players. Positive associations (P < 0.05) were found between 10 m acceleration, maximum velocity, Yo-Yo level Two and 2-km time trial performances and relative distance, and relative distances covered at moderate- and high-intensities during match-play. Moderate relationships were found between vertical jump and relative distance and high-intensity running. Sub-elite Australian football players competing at a higher level exhibit greater physical qualities and match-play activity profiles than lesser-skilled recreational players. Acceleration and maximum velocity, 2-km time trial and Yo-Yo level Two performances discriminate between players of different playing levels, and are related to physical match performance in recreational Australian football. The development of these qualities is likely to contribute to improved match performance in recreational Australian football players. Copyright © 2014 Sports Medicine Australia. Published by Elsevier Ltd. All rights reserved.

Gender differences in nurse practitioner salaries.

PubMed

Greene, Jessica; El-Banna, Majeda M; Briggs, Linda A; Park, Jeongyoung

2017-11-01

While male nurses have been shown to earn considerably more than female nurses, there is less evidence on gender disparities in salary among nurse practitioners (NPs). This study examines whether the gender gap in NP salaries persists after controlling for differences in work setting and demographic factors. We analyzed the relationship between gender and salary (2011 pretax earnings) among 6591 NPs working as NPs at least 35 h per week, using the 2012 National Sample Survey of Nurse Practitioners. We first conducted bivariate regression analyses examining the relationship between gender and earnings, and then developed a multivariate model that controlled for individual differences in demographic and work characteristics. Male NPs earned $12,859 more than female NPs, after adjusting for individual differences in demographics and work characteristics. The gender gap was $7405 for recent NP graduates, and grew over time. Male NPs earned significantly more than female NPs across all clinical specialty areas. The gender disparities in NP salaries documented here regardless of professional seniority or clinical area should spark healthcare organizations to conduct pay equity assessments of their employees' salaries to identify and ameliorate pay inequality. ©2017 American Association of Nurse Practitioners.

Occupational exposure to coal tar pitch volatiles, benzo/a/pyrene and dust in tyre production.

PubMed

Rogaczewska, T; Ligocka, D

1994-01-01

Occupational exposure to coal tar pitch volatiles (CTPVs), benzo/a/pyrene (BaP) and dust was evaluated by means of individual measurements carried out in 80 workers and by stationary measurements on 16 work-posts in two divisions of the tyre producing plant. Dust and coal tar pitch volatiles concentrations in the air were determinated by the gravimetric method, measured, in the case of CPTVs, benzene-soluble fraction (BSF) with ultrasonic extraction. Benzo/a/pyrene analysis was performed using high performance liquid chromato-graphy (HPLC) with a spectrofluorimetric detector. It was found that nearly all personal sampling results for BaP were within the range < 4 divided by 142 ng/m3, except for the exposure of workers employed at weighing the raw materials (3,470-6,060 ng/m3) in the Semiproducts Division. Attention should be paid to the recorded CTPVs concentrations (benzene solubles). About 56% of the Vulcanizing Division workers and about 90% of the Semiproducts Division workers were exposed to these substances at concentrations of over 0.2 mg/m3 (hygienic standard for benzene solubles in USA). Exposure to dust (of high respirable fraction percentage > 90%) which exceeded the admissible value (4 mg/m3) was found mainly only in the workers of the Semiproducts Division at some work-posts.

Evaluation of Motor Gasoline Stability

DTIC Science & Technology

1990-12-01

CAMPINNE AIR POLLUTION CONTROL I LABORATOIRE MECANIQUE TRANSPORT 2565 PLYMOUTH ROAD AVENUE DE LA RENAISSANCE, 3D ANN ARBOR MI 48105 B-1040 BRUSSELS 5...CUARTEL GENERAL DEL EJERCITO ATTN: MR K LAURINSEN ATTN: MAJ M ENGO NOGUES GADHOLTVEJ 11 DIVISION DE LOGISTICA (DIAM/LABCAMVE) DK-9900 FREDERIKSHAVN...Gum for Typical Civilian Gasolines Transported by CEPS and F-46 Gasolines Stored by CEPS ...................................... 47 B. Potential Gum

IFLA General Conference, 1991. Division of Bibliographic Control: Open Forum of Division of Bibliographic Control; Section of Cataloguing; Section of Bibliography; Section of Classification and Indexing. Booklet 4.

ERIC Educational Resources Information Center

International Federation of Library Associations and Institutions, The Hague (Netherlands).

The 11 reports and papers in this booklet were presented at meetings of 4 sections within the Division of Bibliographic Control: (1) "Report of the Section on Cataloguing--Review of the Work 1990/1991" (Inger Cathrine Spangen, Norway); (2) "Les fichiers d'autorite auteurs: Rapport d'activite 1990-1991 (Author Authority Lists: Report…

7 CFR 94.101 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-01-01

... applicant for chemical, physical, or microbiological analyses and tests at a Science and Technology Division... Science and Technology Division laboratory, or by a laboratory approved and recognized by the Division to... quality control of procedures. Official plant or Science and Technology Division laboratories can analyze...

7 CFR 94.101 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-01-01

... applicant for chemical, physical, or microbiological analyses and tests at a Science and Technology Division... Science and Technology Division laboratory, or by a laboratory approved and recognized by the Division to... quality control of procedures. Official plant or Science and Technology Division laboratories can analyze...

7 CFR 94.101 - Definitions.

Code of Federal Regulations, 2012 CFR

2012-01-01

... applicant for chemical, physical, or microbiological analyses and tests at a Science and Technology Division... Science and Technology Division laboratory, or by a laboratory approved and recognized by the Division to... quality control of procedures. Official plant or Science and Technology Division laboratories can analyze...

Staff - Susan S. Seitz | Alaska Division of Geological & Geophysical

Science.gov Websites

, 2017, Geologic Photos of Alaska: Alaska Division of Geological & Geophysical Surveys Digital Data ., Seitz, S.S., and Mulliken, K.M., 2016, Digital compilation of geochemical data for historical samples (presentation): Digital Mapping Techniques Workshop, Champaign, Illinois, May 20-23, 2012: Alaska Division of

RESEARCH AREA -- ARTIFICIAL INTELLIGENCE CONTROL (AIR POLLUTION TECHNOLOGY BRANCH, AIR POLLUTION PREVENTION AND CONTROL DIVISION, NRMRL)

EPA Science Inventory

The Air Pollution Technology Branch (APTB) of NRMRL's Air Pollution Prevention and Control Division in Research Triangle Park, NC, has conducted several research projects for evaluating the use of artificial intelligence (AI) to improve the control of pollution control systems an...

The C. elegans engrailed homolog ceh-16 regulates the self-renewal expansion division of stem cell-like seam cells.

PubMed

Huang, Xinxin; Tian, E; Xu, Yanhua; Zhang, Hong

2009-09-15

Stem cells undergo symmetric and asymmetric division to maintain the dynamic equilibrium of the stem cell pool and also to generate a variety of differentiated cells. The homeostatic mechanism controlling the choice between self-renewal and differentiation of stem cells is poorly understood. We show here that ceh-16, encoding the C. elegans ortholog of the transcription factor Engrailed, controls symmetric and asymmetric division of stem cell-like seam cells. Loss of function of ceh-16 causes certain seam cells, which normally undergo symmetric self-renewal expansion division with both daughters adopting the seam cell fate, to divide asymmetrically with only one daughter retaining the seam cell fate. The human engrailed homolog En2 functionally substitutes the role of ceh-16 in promoting self-renewal expansion division of seam cells. Loss of function of apr-1, encoding the C. elegans homolog of the Wnt signaling component APC, results in transformation of self-renewal maintenance seam cell division to self-renewal expansion division, leading to seam cell hyperplasia. The apr-1 mutation suppresses the seam cell division defect in ceh-16 mutants. Our study reveals that ceh-16 interacts with the Wnt signaling pathway to control the choice between self-renewal expansion and maintenance division and also demonstrates an evolutionarily conserved function of engrailed in promoting cell proliferation.

Publications - RI 2005-1B | Alaska Division of Geological & Geophysical

Science.gov Websites

half of the Solomon C-5 Quadrangle, Seward Peninsula, Alaska Authors: Werdon, M.B., Newberry, R.J publication sales page for more information. Quadrangle(s): Solomon Bibliographic Reference Werdon, M.B area, northern half of the Solomon C-5 Quadrangle, Seward Peninsula, Alaska: Alaska Division of

MORPHOMETRIC ANALYSIS OF RAT HINDLIMB MALFORMATIONS DUE TO GESTATIONAL EXPOSURE TO 5-FU

EPA Science Inventory

SETZER1, R. W., M. D. WILLIAMS2, D. LITTON3, M.G. NAROTSKY4, 1Experimental Toxicology Division and 4Reproductive Toxicology Division, NHEERL, ORD, USEPA, Research Triangle Park, North Carolina; 2UNC School of Medicine, Chapel Hill, North Carolina; and 3Department of Aerospace E...

Publications - RI 2011-3B | Alaska Division of Geological & Geophysical

Science.gov Websites

structural cross sections for the Kavik River map area, Alaska Authors: Wallace, W.K., Wartes, M.A., Decker Kavik River map area, Alaska: Alaska Division of Geological & Geophysical Surveys Report of area, Alaska (144.0 M) Sheet 2 Interpretations of seismic reflection data and structural cross sections

DEVELOPMENTAL TOXICITY OF PERFLUOROOCATANE SULFONATE (PFOS) IN THE RAT AND MOUSE

EPA Science Inventory

1Lau, C., 1J.M. Rogers, 1R.G. Hanson*, 1B.D. Barbee*, 1M.G. Narotsky, 1J.E. Schmid* and 2J.H. Richards*. 1Reproductive Toxicology Division, and 2Environmental Toxicology Division, NHEERL, US EPA, Research Triangle Park, North Carolina. Developmental toxicity of Perfluorooctane ...

77 FR 9706 - Meetings of Humanities Panel

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-17

... submitted to the Scholarly Editions grant program in the Division of Research Programs, at the December 8... Division of Research Programs, at the December 8, 2011 deadline. 3. Date: March 20, 2012. Time: 8:30 a.m. to 5 p.m. Room: 315. Program: This meeting will review applications on the subject of Philosophy and...

Patterns of cell division, DNA base compositions, and fine structures of some radiation-resistant vegetative bacteria found in food

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sanders, S.W.; Maxcy, R.B.

1979-01-01

Representative highly radiation-resistant Moraxella-Acinetobacter (M-A), Pseudomonas radiora, Micrococcus radiodurans, and Micrococcus radiophilus exhibited a wide variety of division systems and cell wall characteristics. However, the more resistant M-A possessed unusually thick cell walls, indicating a possible role of the cell wall in radiation resistance in the M-A. Thick septation was present in most of the bacteria studied, but was absent in P. radiora, thus excluding this as a necessity for high resistance. Reliable determination of the number of division planes of the M-A for use as a taxonomic criterion was achieved by the direct observation of dividing cells. The highlymore » resistant M-A were found to divide in multiple planes and had base compositions of 54.0 to 57.5%, unlike typical Moraxella and/or Acinetobacter species. The taxonomic position of most highly resistant bacteria remains unclear.« less

Images multiplexing by code division technique

NASA Astrophysics Data System (ADS)

Kuo, Chung J.; Rigas, Harriett

Spread Spectrum System (SSS) or Code Division Multiple Access System (CDMAS) has been studied for a long time, but most of the attention was focused on the transmission problems. In this paper, we study the results when the code division technique is applied to the image at the source stage. The idea is to convolve the N different images with the corresponding m-sequence to obtain the encrypted image. The superimposed image (summation of the encrypted images) is then stored or transmitted. The benefit of this is that no one knows what is stored or transmitted unless the m-sequence is known. The recovery of the original image is recovered by correlating the superimposed image with corresponding m-sequence. Two cases are studied in this paper. First, the two-dimensional image is treated as a long one-dimensional vector and the m-sequence is employed to obtain the results. Secondly, the two-dimensional quasi m-array is proposed and used for the code division multiplexing. It is shown that quasi m-array is faster when the image size is 256 x 256. The important features of the proposed technique are not only the image security but also the data compactness. The compression ratio depends on how many images are superimposed.

Images Multiplexing By Code Division Technique

NASA Astrophysics Data System (ADS)

Kuo, Chung Jung; Rigas, Harriett B.

1990-01-01

Spread Spectrum System (SSS) or Code Division Multiple Access System (CDMAS) has been studied for a long time, but most of the attention was focused on the transmission problems. In this paper, we study the results when the code division technique is applied to the image at the source stage. The idea is to convolve the N different images with the corresponding m-sequence to obtain the encrypted image. The superimposed image (summation of the encrypted images) is then stored or transmitted. The benefit of this is that no one knows what is stored or transmitted unless the m-sequence is known. The recovery of the original image is recovered by correlating the superimposed image with corresponding m-sequence. Two cases are studied in this paper. First, the 2-D image is treated as a long 1-D vector and the m-sequence is employed to obtained the results. Secondly, the 2-D quasi m-array is proposed and used for the code division multiplexing. It is showed that quasi m-array is faster when the image size is 256x256. The important features of the proposed technique are not only the image security but also the data compactness. The compression ratio depends on how many images are superimposed.

Characterization of dependencies between growth and division in budding yeast

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mayhew, Michael B.; Iversen, Edwin S.; Hartemink, Alexander J.

Cell growth and division are processes vital to the proliferation and development of life. Coordination between these two processes has been recognized for decades in a variety of organisms. In the budding yeast Saccharomyces cerevisiae, this coordination or ‘size control’ appears as an inverse correlation between cell size and the rate of cell-cycle progression, routinely observed in G1 prior to cell division commitment. Beyond this point, cells are presumed to complete S/G 2/M at similar rates and in a size-independent manner. As such, studies of dependence between growth and division have focused on G1. Moreover, in unicellular organisms, coordination betweenmore » growth and division has commonly been analyzed within the cycle of a single cell without accounting for correlations in growth and division characteristics between cycles of related cells. In a comprehensive analysis of three published time-lapse microscopy datasets, we analyze both intra- and inter-cycle dependencies between growth and division, revisiting assumptions about the coordination between these two processes. Interestingly, we find evidence (1) that S/G 2/M durations are systematically longer in daughters than in mothers, (2) of dependencies between S/G2/M and size at budding that echo the classical G1 dependencies, and, (3) in contrast with recent bacterial studies, of negative dependencies between size at birth and size accumulated during the cell cycle. In addition, we develop a novel hierarchical model to uncover inter-cycle dependencies, and we find evidence for such dependencies in cells growing in sugar-poor environments. Our analysis highlights the need for experimentalists and modelers to account for new sources of cell-to-cell variation in growth and division, and our model provides a formal statistical framework for the continued study of dependencies between biological processes.« less

Characterization of dependencies between growth and division in budding yeast

DOE PAGES

Mayhew, Michael B.; Iversen, Edwin S.; Hartemink, Alexander J.

2017-02-01

Cell growth and division are processes vital to the proliferation and development of life. Coordination between these two processes has been recognized for decades in a variety of organisms. In the budding yeast Saccharomyces cerevisiae, this coordination or ‘size control’ appears as an inverse correlation between cell size and the rate of cell-cycle progression, routinely observed in G1 prior to cell division commitment. Beyond this point, cells are presumed to complete S/G 2/M at similar rates and in a size-independent manner. As such, studies of dependence between growth and division have focused on G1. Moreover, in unicellular organisms, coordination betweenmore » growth and division has commonly been analyzed within the cycle of a single cell without accounting for correlations in growth and division characteristics between cycles of related cells. In a comprehensive analysis of three published time-lapse microscopy datasets, we analyze both intra- and inter-cycle dependencies between growth and division, revisiting assumptions about the coordination between these two processes. Interestingly, we find evidence (1) that S/G 2/M durations are systematically longer in daughters than in mothers, (2) of dependencies between S/G2/M and size at budding that echo the classical G1 dependencies, and, (3) in contrast with recent bacterial studies, of negative dependencies between size at birth and size accumulated during the cell cycle. In addition, we develop a novel hierarchical model to uncover inter-cycle dependencies, and we find evidence for such dependencies in cells growing in sugar-poor environments. Our analysis highlights the need for experimentalists and modelers to account for new sources of cell-to-cell variation in growth and division, and our model provides a formal statistical framework for the continued study of dependencies between biological processes.« less

Characterization of dependencies between growth and division in budding yeast

PubMed Central

Iversen, Edwin S.; Hartemink, Alexander J.

2017-01-01

Cell growth and division are processes vital to the proliferation and development of life. Coordination between these two processes has been recognized for decades in a variety of organisms. In the budding yeast Saccharomyces cerevisiae, this coordination or ‘size control’ appears as an inverse correlation between cell size and the rate of cell-cycle progression, routinely observed in G1 prior to cell division commitment. Beyond this point, cells are presumed to complete S/G2/M at similar rates and in a size-independent manner. As such, studies of dependence between growth and division have focused on G1. Moreover, in unicellular organisms, coordination between growth and division has commonly been analysed within the cycle of a single cell without accounting for correlations in growth and division characteristics between cycles of related cells. In a comprehensive analysis of three published time-lapse microscopy datasets, we analyse both intra- and inter-cycle dependencies between growth and division, revisiting assumptions about the coordination between these two processes. Interestingly, we find evidence (i) that S/G2/M durations are systematically longer in daughters than in mothers, (ii) of dependencies between S/G2/M and size at budding that echo the classical G1 dependencies, and (iii) in contrast with recent bacterial studies, of negative dependencies between size at birth and size accumulated during the cell cycle. In addition, we develop a novel hierarchical model to uncover inter-cycle dependencies, and we find evidence for such dependencies in cells growing in sugar-poor environments. Our analysis highlights the need for experimentalists and modellers to account for new sources of cell-to-cell variation in growth and division, and our model provides a formal statistical framework for the continued study of dependencies between biological processes. PMID:28228543

The R and M 2000 Process and Reliability and Maintainability Management: Attitudes of Senior Level Managers in Aeronautical Systems Division

DTIC Science & Technology

1988-09-01

I .. I . . .. . - - AFIT/GLM/LSM/88S-59 THE R& M 2000 PROCESS AND RELIABILITY AND MAINTAINABILITY...respondents provided verbal responses to this question. Although one-half of these responses spoke 65 k - ’ ’ ’ i l l I l l i favorably of R& M 2000 , there were...GROUP SUB-GROUP Attitudes, Reliability, Maintainability, R& M , R&" 2000 , 05 01 I Aeronautical Systems Division, ASD 19. ABSTRACT (Continue on

Auxin-Dependent Cell Division and Cell Elongation. 1-Naphthaleneacetic Acid and 2,4-Dichlorophenoxyacetic Acid Activate Different Pathways1

PubMed Central

Campanoni, Prisca; Nick, Peter

2005-01-01

During exponential phase, the tobacco (Nicotiana tabacum) cell line cv Virginia Bright Italia-0 divides axially to produce linear cell files of distinct polarity. This axial division is controlled by exogenous auxin. We used exponential tobacco cv Virginia Bright Italia-0 cells to dissect early auxin signaling, with cell division and cell elongation as physiological markers. Experiments with 1-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D) demonstrated that these 2 auxin species affect cell division and cell elongation differentially; NAA stimulates cell elongation at concentrations that are much lower than those required to stimulate cell division. In contrast, 2,4-D promotes cell division but not cell elongation. Pertussis toxin, a blocker of heterotrimeric G-proteins, inhibits the stimulation of cell division by 2,4-D but does not affect cell elongation. Aluminum tetrafluoride, an activator of the G-proteins, can induce cell division at NAA concentrations that are not permissive for division and even in the absence of any exogenous auxin. The data are discussed in a model where the two different auxins activate two different pathways for the control of cell division and cell elongation. PMID:15734918

Biorepositories | Division of Cancer Prevention

Cancer.gov

Carefully collected and controlled high-quality human biospecimens, annotated with clinical data and properly consented for investigational use, are available through the Division of Cancer Prevention Biorepositories listed in the charts below. Biorepositories Managed by the Division of Cancer Prevention Biorepositories Supported by the Division of Cancer Prevention Related

Instrumentation and Controls Division Overview: Sensors Development for Harsh Environments at Glenn Research Center

NASA Technical Reports Server (NTRS)

Zeller, Mary V.; Lei, Jih-Fen

2002-01-01

The Instrumentation and Controls Division is responsible for planning, conducting and directing basic and applied research on advanced instrumentation and controls technologies for aerospace propulsion and power applications. The Division's advanced research in harsh environment sensors, high temperature high power electronics, MEMS (microelectromechanical systems), nanotechnology, high data rate optical instrumentation, active and intelligent controls, and health monitoring and management will enable self-feeling, self-thinking, self-reconfiguring and self-healing Aerospace Propulsion Systems. These research areas address Agency challenges to deliver aerospace systems with reduced size and weight, and increased functionality and intelligence for future NASA missions in advanced aeronautics, economical space transportation, and pioneering space exploration. The Division also actively supports educational and technology transfer activities aimed at benefiting all humankind.

Improvement of Fish Sauce Quality by Strain CMC5-3-1: A Novel Species of Staphylococcus sp.

PubMed

Udomsil, Natteewan; Rodtong, Sureelak; Tanasupawat, Somboon; Yongsawatdigul, Jirawat

2015-09-01

Staphylococcus sp. CMC5-3-1 and CMS5-7-5 isolated from fermented fish sauce at 3 to 7 mo, respectively, showed different characteristics on protein hydrolysis and volatile formation. These Gram-positive cocci were able to grow in up to 15% NaCl with the optimum at 0.5% to 5% NaCl in tryptic soy broth. Based on ribosomal 16S rRNA gene sequences, Staphylococcus sp. CMC5-3-1 and CMS5-7-5 showed 99.0% similarity to that of Staphylococcus piscifermentans JCM 6057(T) , but DNA-DNA relatedness was <30%, indicating that they were likely to be new species. DNA relatedness between these 2 strains was only 65%, suggesting that they also belonged to different species. The α-amino group content of 6-month-old fish sauce inoculated with Staphylococcus sp. CMC5-3-1 was 740.5 mM, which was higher than that inoculated by the strain CMS5-7-5 (662.14 mM, P < 0.05). Histamine was not produced during fermentations with both strains. Fish sauce inoculated with Staphylococcus sp. CMC5-3-1 showed the highest content of total glutamic acid (P < 0.05). The major volatile compound detected in fish sauce inoculated with Staphylococcus sp. CMC5-3-1 was 2-methypropanal, contributing to the desirable dark chocolate note. Staphylococcus sp. CMC5-3-1 could be applied as a starter culture to improve the umami and aroma of fish sauce. © 2015 Institute of Food Technologists®

Comparison of Chosen Physical Fitness Characteristics of Turkish Professional Basketball Players by Division and Playing Position

PubMed Central

Köklü, Yusuf; Alemdaroğlu, Utku; Koçak, Fatma Ünver; Erol, A. Emre; Fındıkoğlu, Gülin

2011-01-01

The purpose of the present study was to compare chosen physical fitness characteristics of Turkish professional basketball players in different divisions (first and second division) and playing positions. Forty-five professional male basketball players (14 guards, 15 forwards, 16 centers) participated in this study voluntarily. For each player, anthropometric measurements were performed, as well as a multi-stage 20 m shuttle run, isokinetic leg strength, squat jump (SJ), countermovement jump (CMJ), 10–30 meter single-sprint and T-drill agility tests. The differences in terms of division were evaluated by independent t-test and the differences by playing position were evaluated by one-way ANOVA with Post Hoc Tukey test. First division players’ CMJ measurements were significantly higher than those of second division players’ (p≤0.05), whereas second division players’ 10 m sprint times were significantly better than those of first division players’ (p≤0.05). In addition, forwards and centers were significantly taller than guards. Centers were significantly heavier and their T-drill test performances were inferior to those of forwards and guards (p≤0.05). Moreover, guards had a significantly higher maximal oxygen uptake (VO2 max) than centers. Guards and forwards showed significantly better performance in the 10 and 30 m sprint tests than centers (p≤0.05). Forwards and centers had significantly better left leg flexor strength at 180°.s−1(p≤0.05). In conclusion, the findings of the present study indicated that physical performance of professional basketball players differed among guards, forwards and centers, whereas there were not significant differences between first and second division players. According to the present study, court positions have different demands and physical attributes which are specific to each playing position in professional basketball players. Therefore, these results suggest that coaches should tailor fitness programs according to specific positions on the court. PMID:23486863

Comparison of chosen physical fitness characteristics of Turkish professional basketball players by division and playing position.

PubMed

Köklü, Yusuf; Alemdaroğlu, Utku; Koçak, Fatma Ünver; Erol, A Emre; Fındıkoğlu, Gülin

2011-12-01

The purpose of the present study was to compare chosen physical fitness characteristics of Turkish professional basketball players in different divisions (first and second division) and playing positions. Forty-five professional male basketball players (14 guards, 15 forwards, 16 centers) participated in this study voluntarily. For each player, anthropometric measurements were performed, as well as a multi-stage 20 m shuttle run, isokinetic leg strength, squat jump (SJ), countermovement jump (CMJ), 10-30 meter single-sprint and T-drill agility tests. The differences in terms of division were evaluated by independent t-test and the differences by playing position were evaluated by one-way ANOVA with Post Hoc Tukey test. First division players' CMJ measurements were significantly higher than those of second division players' (p≤0.05), whereas second division players' 10 m sprint times were significantly better than those of first division players' (p≤0.05). In addition, forwards and centers were significantly taller than guards. Centers were significantly heavier and their T-drill test performances were inferior to those of forwards and guards (p≤0.05). Moreover, guards had a significantly higher maximal oxygen uptake (VO2 max) than centers. Guards and forwards showed significantly better performance in the 10 and 30 m sprint tests than centers (p≤0.05). Forwards and centers had significantly better left leg flexor strength at 180°.s(-1)(p≤0.05). In conclusion, the findings of the present study indicated that physical performance of professional basketball players differed among guards, forwards and centers, whereas there were not significant differences between first and second division players. According to the present study, court positions have different demands and physical attributes which are specific to each playing position in professional basketball players. Therefore, these results suggest that coaches should tailor fitness programs according to specific positions on the court.

ANALYTICAL CHEMISTRY DIVISION ANNUAL PROGRESS REPORT FOR PERIOD ENDING DECEMBER 31, 1961

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

1962-02-01

Research and development progress is reported on analytlcal instrumentation, dlssolver-solution analyses, special research problems, reactor projects analyses, x-ray and spectrochemical analyses, mass spectrometry, optical and electron microscopy, radiochemical analyses, nuclear analyses, inorganic preparations, organic preparations, ionic analyses, infrared spectral studies, anodization of sector coils for the Analog II Cyclotron, quality control, process analyses, and the Thermal Breeder Reactor Projects Analytical Chemistry Laboratory. (M.C.G.)

Joint Service Chemical and Biological Defense Program. FY00-02 Overview

DTIC Science & Technology

2001-09-01

Development. Contractors: 12 BI DS Biological Integrated Detection System (BIDS) Lead Service Bio Road HERCULES, CA Bruker Analytical Systems BILLERICA, MA...Dynamics Land Systems Division DETROIT, MI Henschel Wehrtechnik GERMANY Bruker -Franzen GERMANY Block II – TBD Milestones Block I MS III (2QFY94) Block...ground. Accessories include hoses and hose reels, two trigger-controlled spray wands , and two electrical-powered scrub brush assemblies. The M22

77 FR 29231 - Oklahoma: Incorporation by Reference of Approved State Hazardous Waste Management Program

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-17

... Oversight Section (6PD-O), Multimedia Planning and Permitting Division, EPA Region 6, 1445 Ross Avenue... Permitting Division, EPA Region 6, 1445 Ross Avenue, Dallas, Texas 75202-2733. Instructions: Direct your....m. to 4:00 p.m. Monday through Friday at the following location: EPA Region 6, 1445 Ross Avenue...

Publications - GMC 195 | Alaska Division of Geological & Geophysical

Science.gov Websites

Oil NPRA Tunalik #1 well, Western Alaska Authors: Worrall, D.M., and Shell Oil Company Publication Reference Worrall, D.M., and Shell Oil Company, 1992, Evaluation of basalt samples (17,859-17,888') from the Husky Oil NPRA Tunalik #1 well, Western Alaska: Alaska Division of Geological & Geophysical Surveys

Calibration of hydrometers

NASA Astrophysics Data System (ADS)

Lorefice, Salvatore; Malengo, Andrea

2006-10-01

After a brief description of the different methods employed in periodic calibration of hydrometers used in most cases to measure the density of liquids in the range between 500 kg m-3 and 2000 kg m-3, particular emphasis is given to the multipoint procedure based on hydrostatic weighing, known as well as Cuckow's method. The features of the calibration apparatus and the procedure used at the INRiM (formerly IMGC-CNR) density laboratory have been considered to assess all relevant contributions involved in the calibration of different kinds of hydrometers. The uncertainty is strongly dependent on the kind of hydrometer; in particular, the results highlight the importance of the density of the reference buoyant liquid, the temperature of calibration and the skill of operator in the reading of the scale in the whole assessment of the uncertainty. It is also interesting to realize that for high-resolution hydrometers (division of 0.1 kg m-3), the uncertainty contribution of the density of the reference liquid is the main source of the total uncertainty, but its importance falls under about 50% for hydrometers with a division of 0.5 kg m-3 and becomes somewhat negligible for hydrometers with a division of 1 kg m-3, for which the reading uncertainty is the predominant part of the total uncertainty. At present the best INRiM result is obtained with commercially available hydrometers having a scale division of 0.1 kg m-3, for which the relative uncertainty is about 12 × 10-6.

Quality of life and objective outcome assessment in women with tape division after surgery for stress urinary incontinence

PubMed Central

Ulrich, Daniela; Bjelic-Radisic, Vesna; Höllein, Anna; Tamussino, Karl; Aigmüller, Thomas

2017-01-01

Background Midurethral tapes may cause long-term complications such as voiding dysfunction, groin pain, de novo urgency or mesh erosion, which necessitate a reoperation. There is a paucity of data regarding health related quality of life in patients undergoing tape removal. The aim of the study was to evaluate quality of life (QoL) and objective outcome after midurethral tape division or excision. Methods All patients who underwent a midurethral tape division for voiding difficulties, pain or therapy resistant de novo overactive bladder between 1999 and 2014 were invited for follow-up. A control group with a suburethral tape without division was established in a 1:2 ratio and matched for age, tape used and year of tape insertion. Patients completed the Kings´ Health Questionnaire (KHQ), Incontinence Outcome Questionnaire, Female Sexual Function Index Questionnaire and the Patient Global Impression of Improvement score. Results Tape division or excision was performed in 32 women. Overall, 15 (60%) of 25 women who were alive were available for clinical examination and completed the questionnaires. Tape division was performed for voiding dysfunction (n = 7), overactive bladder (n = 2), mesh extrusion (n = 3) and ongoing pain (n = 3). Median time to tape division/excision was 10 months. Three women in the tape division group had undergone reoperation for stress urinary incontinence (SUI). At a median follow-up of 11 years (IQR 9–13) subjective SUI rate was 53% (8/15 women) in the tape division group and 17% (5/30) in the control group (p = 0.016), with no significant differences in objective SUI rates between groups. With regard to quality of life, the study group had significantly worse scores in the SUI related domains role limitation, physical limitation, severity measures and social limitations (KHQ) compared to the control group. Conclusions Women needing tape division or excision have lower SUI related QoL scores compared to controls mostly because of higher subjective SUI rates. PMID:28346541

Publications - NL 2002-1 | Alaska Division of Geological & Geophysical

Science.gov Websites

Visiting Alaska State Employees DGGS State of Alaska search Alaska Division of Geological & Geophysical 2002 Publisher: Alaska Division of Geological & Geophysical Surveys Ordering Info: Download below Reference DGGS Staff, and Werdon, M.B., 2002, Alaska GeoSurvey News - Geologic Investigations in the Salcha

Robust control of mitotic spindle orientation in the developing epidermis

PubMed Central

Poulson, Nicholas D.

2010-01-01

Progenitor cells must balance self-amplification and production of differentiated progeny during development and homeostasis. In the epidermis, progenitors divide symmetrically to increase surface area and asymmetrically to promote stratification. In this study, we show that individual epidermal cells can undergo both types of division, and therefore, the balance is provided by the sum of individual cells’ choices. In addition, we define two control points for determining a cell’s mode of division. First is the expression of the mouse Inscuteable gene, which is sufficient to drive asymmetric cell division (ACD). However, there is robust control of division orientation as excessive ACDs are prevented by a change in the localization of NuMA, an effector of spindle orientation. Finally, we show that p63, a transcriptional regulator of stratification, does not control either of these processes. These data have uncovered two important regulatory points controlling ACD in the epidermis and allow a framework for analysis of how external cues control this important choice. PMID:21098114

IFT Proteins Accumulate during Cell Division and Localize to the Cleavage Furrow in Chlamydomonas

PubMed Central

Wood, Christopher R.; Wang, Zhaohui; Diener, Dennis; Zones, James Matt; Rosenbaum, Joel; Umen, James G.

2012-01-01

Intraflagellar transport (IFT) proteins are well established as conserved mediators of flagellum/cilium assembly and disassembly. However, data has begun to accumulate in support of IFT protein involvement in other processes elsewhere in the cell. Here, we used synchronous cultures of Chlamydomonas to investigate the temporal patterns of accumulation and localization of IFT proteins during the cell cycle. Their mRNAs showed periodic expression that peaked during S and M phase (S/M). Unlike most proteins that are synthesized continuously during G1 phase, IFT27 and IFT46 levels were found to increase only during S/M phase. During cell division, IFT27, IFT46, IFT72, and IFT139 re-localized from the flagella and basal bodies to the cleavage furrow. IFT27 was further shown to be associated with membrane vesicles in this region. This localization pattern suggests a role for IFT in cell division. PMID:22328921

The Petsamo-Kirkenes Operation: Soviet Breakthrough and Pursuit in the Arctic, October 1944 (Leavenworth Papers, Number 17)

DTIC Science & Technology

1989-09-01

Roschmann , "Small Unit Tactics, Combats in Taiga and Tundra," Foreign Military Studies no. MS P-060m (Historical Division, U.S. Army, Europe, 1952), 148...Brockelmann, Klaus, and Hans Roschmann . "Small Unit Tactics, Combats in Taiga and Tundra." Foreign Military Studies no. MS P-060m. Historical Division

77 FR 50198 - Self-Regulatory Organizations; The Fixed Income Clearing Corporation; Notice of Filing Proposed...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-20

... Time at Which the Mortgage-Backed Securities Division Runs Its Daily Morning Pass August 14, 2012... Division (``MBSD'') runs its first processing pass of the day from 2 p.m. to 4 p.m. Eastern Standard Time... MBSD intends to move the time at which it runs its first processing pass of the day (historically...

Publications - SR 48 | Alaska Division of Geological & Geophysical Surveys

Science.gov Websites

and Facilities Staff Seismic and Well Data Data Reports Contact Us Frequently Asked Questions Ask a , R.C., Clough, A.H., Henning, M.W., and Hansen, E.W. Publication Date: 1994 Publisher: Alaska Division ., Swainbank, R.C., Clough, A.H., Henning, M.W., and Hansen, E.W., 1994, Alaska's mineral industry 1993: Alaska

Publications - SR 49 | Alaska Division of Geological & Geophysical Surveys

Science.gov Websites

and Facilities Staff Seismic and Well Data Data Reports Contact Us Frequently Asked Questions Ask a , T.K., Clough, A.H., Henning, M.W., and Hansen, E.W. Publication Date: 1995 Publisher: Alaska Division ., Bundtzen, T.K., Clough, A.H., Henning, M.W., and Hansen, E.W., 1995, Alaska's mineral industry 1994: Alaska

Laboratory Measurements of Cometary Photochemical Phenomena.

DTIC Science & Technology

1981-12-04

PROGFIAM ELEMENT.PROJECT TASK Laser .Chemistry Division AREA & WORK UNIT NUMaZRS Department of Chemistry - Howard University NR.051-733 Wash’ ngtQn, D. C...William M. Jackson Laser Chemistry Division Department of Chemistry Howard University .Washington, D. C. 20059 / Published by Jet Propulsion Laboratory...MEASUREMENTS OF COMETARY PHOTOCHEMICAL PHENOMENA William M. Jackson Howard University Washington, DC 20059 Abstract Laboratory experiments are described

IFLA General Conference, 1989. Division of Bibliographic Control. Open Forum of the Division; Section on Cataloguing; Section on Bibliography; Section on Classification and Indexing. Booklet 40.

ERIC Educational Resources Information Center

International Federation of Library Associations, The Hague (Netherlands).

There are 16 papers in this collection from the Division of Bibliographic Control: (1) "Report from the Section on Cataloging" (Tom Delsey); (2) "Section on Bibliography Report" (B. C. Bloomfield); (3) "Report from the Section on Classification and Indexing" (Robert P. Holley); (4) "The Cost of Cataloging" (French and English versions; Genevieve…

75 FR 24748 - Johnson Controls, Inc., Automotive Experience Division, Including Workers Whose Unemployment...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-05

...., Automotive Experience Division, Including Workers Whose Unemployment Insurance (UI) Wages Are Paid Through... Assistance on October 6, 2009, applicable to workers of Johnson Controls, Inc., Automotive Experience... industry. New information shows that Johnson Controls purchased Hoover Universal in 1985 and that some...

Acoustic Directivity Patterns for Army Weapons

DTIC Science & Technology

1979-01-01

work was performed by the Environmental Division (EN), u.S. Army Construction Engineering Research Laboratory (CERL). Dr. R. K. Jain is Chief of EN...V) P.0. Schomer,,, L. M./Little I rTPRFORMING ORGANI ZATION NAME AND ADDRESS 0.PROG3RAM ELEMENT. PPOJECT, TAWF U.S. ARMY AREA & WORK UNIT NUMBERS...34Environmental Quality for Construction and Operation of Military Facilities" Task 03, "Pollution Control Technology" and Work Unit 001, "Prediction of

History of the Waterways Experiment Station

DTIC Science & Technology

1968-06-01

Washington Headquarters Services , Directorate for Information Operations and Reports, 1215 Jefferson Davis Highway, Suite 1204, Arlington VA 22202-4302...Soils Division; G. L. Arbuthnot, Jr., Chief, Nuclear Weapons Effects Division; C. B. Patterson, Chief, Technical Services Division; J. M. McCaskill...Chief, Reproduction and Reports Branch; J. S. Taylor, Jr. , Chief, Office of Administfative Services ; J. W. Pettigrew, Chief, Safety Office; E. P

An Analysis of the Perceptions of Division Senior Combat Arms Leaders of the Tactical Competence of Division Signal Officers and Assistant Division Signal Officers.

DTIC Science & Technology

1987-06-05

field the equipment ( Valletta , 1980). The need for signal officers to be trained to employ new communications systems in support of the tactical...Gordon, GAO 30905). Valletta , A. M. (1980). Tactical automnation-communications: Supporting corps rear to front line. Army Magazine, 1(4), 25-28

Ventilation and perfusion imaging by electrical impedance tomography: a comparison with radionuclide scanning.

PubMed

Kunst, P W; Vonk Noordegraaf, A; Hoekstra, O S; Postmus, P E; de Vries, P M

1998-11-01

Electrical impedance tomography (EIT) is a technique that makes it possible to measure ventilation and pulmonary perfusion in a volume that approximates to a 2D plane. The possibility of using EIT for measuring the left-right division of ventilation and perfusion was compared with that of radionuclide imaging. Following routine ventilation (81mKr) and perfusion scanning (99mTc-MAA), EIT measurements were performed at the third and the sixth intercostal level in 14 patients with lung cancer. A correlation (r = 0.98, p < 0.005) between the left-right division for the ventilation measured with EIT and that with 81mKr was found. For the left-right division of pulmonary perfusion a correlation of 0.95 (p < 0.005) was found between the two methods. The reliability coefficient (RC) was calculated for estimating the left-right division with EIT. The RC for the ventilation measurements was 94% and 96% for the perfusion measurements. The correlation analysis for reproducibility of the EIT measurements was 0.95 (p < 0.001) for the ventilation and 0.93 (p < 0.001) for the perfusion measurements. In conclusion, EIT can be regarded as a promising technique to estimate the left-right division of pulmonary perfusion and ventilation.

Investigation of controlled flight into terrain : descriptions of flight paths for selected controlled flight into terrain (CFIT) aircraft accidents, 1985-1997

DOT National Transportation Integrated Search

1999-03-01

This report documents an investigation of the flight paths of 13 selected controlled flight into terrain (CFIT) aircraft accidents that occurred between 1985 and 1997. The Operations Assessment Division (DTS-43) and the Aviation Safety Division (DTS-...

Regulation of transcription of the cell division gene ftsA during sporulation of Bacillus subtilis.

PubMed Central

Gholamhoseinian, A; Shen, Z; Wu, J J; Piggot, P

1992-01-01

Three distinct 5' ends of ftsA mRNA were identified by S1 mapping and by primer extension analysis. These are thought to represent three transcription start sites. The transcripts from the downstream and upstream sites were detected throughout growth. The transcript from the middle site was not detected during exponential growth but was detected within 30 min of the start of sporulation, when it was the predominant transcript. Insertion of a cat cassette in the middle promoter, ftsAp2 (p2), did not affect vegetative growth but prevented postexponential symmetrical division and spore formation. Transcription from p2 was dependent on RNA polymerase containing sigma H, and promoter p2 resembled the consensus sigma H promoter. Transcription from p2 did not require expression of the spo0A, spo0B, spo0E, spo0F, or spo0K loci. Northern (RNA) blot analysis indicated that ftsA is cotranscribed with the adjacent ftsZ gene. Multiple promoters provide a mechanism by which essential vegetative genes can be subjected to sporulation control independent of control during vegetative growth. In the case of ftsA,Z, the promoters provide a mechanism to permit septum formation in conditions of nutrient depletion that might be expected to shut down the vegetative division machinery. Images PMID:1624452

The Effectiveness of Power Tool Cleaning as an Alternative to Abrasive Blasting

DTIC Science & Technology

1995-06-01

Lamar Avenue Houston, TX 77251 3M Building Service and Cleaning Products Division Building 223-65-03 St. Paul, MN 55144-1000 Marindus Company P. O...Mill Scale Removal with 3M Brand Heavy Duty RotoPeen Flap Wheel”;”Scotch-Brite” Surface Conditioning Products, 3M Building Service and Cleaning ... Products Division. 18. “The Dilemma of Removing Lead-Based Paint,” January 1988, p. 34-35. Specifications: ASTM TEST METHODS: B1 17, Salt Spray (Fog

Instrumentation and Controls Division progress report for the period July 1, 1986 to June 30, 1988

DOE Office of Scientific and Technical Information (OSTI.GOV)

Klobe, L.E.

1988-12-01

The Instrumentation and Controls (IandC) Division of Oak Ridge National Laboratory (ORNL) performs basic and applied instrumentation and controls research, development and design engineering, specialized instrument design and fabrication, and maintenance services for instruments, electronics, and computers. The IandC Division is one of the largest RandD organizations of its type among government laboratories, and it exists as the result of an organizational strategy to integrate ORNL's instrumentation and controls-related disciplines into one dedicated functional organization to increase the Laboratory's expertise and capabilities in these rapidly expanding, innovative areas of technology. The Division participates in the programs and projects of ORNLmore » by applying its expertise and capabilities in concert with other divisions to perform basic research and mission-oriented technology development. Many of the Division's RandD tasks that are a part of a larger ORNL program are of sufficient scope that the IandC effort constitutes a separate program element with direct funding and management responsibility within the Division. The activities of IandC include performance of an RandD task in IandC facilities, the participation of from one of many IandC engineers and scientists in a multidisciplinary team working in a specific research area or development project, design and fabrication of a special instrument or instrumentation system, or a few hours of maintenance service. In its support and maintenance work, the role of the IandC Division is to provide a level of expertise appropriate to complete a job successfully at minimum overall cost and time schedule---a role which involves IandC in almost all ORNL activities.« less

Directed self-assembly of virus particles at nanoscale chemical templates

NASA Astrophysics Data System (ADS)

Chung, Sung-Wook; Cheung, Chin Li; Chatterji, Anju; Lin, Tianwei; Johnson, Jack; de Yoreo, Jim

2006-03-01

Because viruses can be site-specifically engineered to present catalytic, electronic, and optical moieties, they are attractive as building blocks for hierarchical nanostructures. We report results using scanned probe nanolithography to direct virus organization into 1D and 2D patterns and in situ AFM investigations of organization dynamics as pattern geometry, inter-viral potential, virus flux, and virus-pattern interaction are varied. Cowpea Mosaic Virus was modified to present surface sites with histidine (His) or cysteine (Cys) groups. Flat gold substrates were patterned with 10-100nm features of alkyl thiols terminated by Ni-NTA or meleimide groups to reversibly and irreversibly bind to the Hys and Cys groups, respectively. We show how assembly kinetics, degree of ordering and cluster-size distribution at these templates depend on the control parameters and present a physical picture of virus assembly at templates that incorporates growth dynamics of small-molecule epitaxial systems and condensation dynamics of colloidal systems. This work was performed under the auspices of the U. S. Department of Energy by the University of California, Lawrence Livermore National Laboratory under Contract No. W-7405-Eng-48.

Modeling Hohlraum-Based Laser Plasma Instability Experiments

NASA Astrophysics Data System (ADS)

Meezan, N. B.

2005-10-01

Laser fusion targets must control laser-plasma instabilities (LPI) in order to perform as designed. We present analyses of recent hohlraum LPI experiments from the Omega laser facility. The targets, gold hohlraums filled with gas or SiO2 foam, are preheated by several 3φ beams before an interaction beam (2φ or 3φ) is fired along the hohlraum axis. The experiments are simulated in 2-D and 3-D using the code hydra. The choice of electron thermal conduction model in hydra strongly affects the simulated plasma conditions. This work is part of a larger effort to systematically explore the usefulness of linear gain as a design tool for fusion targets. We find that the measured Raman and Brillouin backscatter scale monotonically with the peak linear gain calculated for the target; however, linear gain is not sufficient to explain all trends in the data. This work was performed under the auspices of the U.S. Department of Energy by the University of California Lawrence Livermore National Laboratory under contract No. W-7405-ENG-48.

Smoothing Polymer Surfaces by Solvent-Vapor Exposure

NASA Astrophysics Data System (ADS)

Anthamatten, Mitchell

2003-03-01

Ultra-smooth polymer surfaces are of great importance in a large body of technical applications such as optical coatings, supermirrors, waveguides, paints, and fusion targets. We are investigating a simple approach to controlling surface roughness: by temporarily swelling the polymer with solvent molecules. As the solvent penetrates into the polymer, its viscosity is lowered, and surface tension forces drive surface flattening. To investigate sorption kinetics and surface-smoothing phenomena, a series of vapor-deposited poly(amic acid) films were exposed to dimethyl sulfoxide vapors. During solvent exposure, the surface topology was continuously monitored using light interference microscopy. The resulting power spectra indicate that high-frequency defects smooth faster than low-frequency defects. This frequency dependence was studied by depositing polymer films onto a series of 2D sinusoidal surfaces and performing smoothing experiments. Results show that the amplitudes of the sinusoidal surfaces decay exponentially with solvent exposure time, and the exponential decay constants are proportional to surface frequency. This work was performed under the auspices of the U.S. Department of Energy by the University of California Lawrence Livermore National Laboratory under contract No. W-7405-Eng-48.

Polarity, cell division, and out-of-equilibrium dynamics control the growth of epithelial structures

PubMed Central

Cerruti, Benedetta; Puliafito, Alberto; Shewan, Annette M.; Yu, Wei; Combes, Alexander N.; Little, Melissa H.; Chianale, Federica; Primo, Luca; Serini, Guido; Mostov, Keith E.; Celani, Antonio

2013-01-01

The growth of a well-formed epithelial structure is governed by mechanical constraints, cellular apico-basal polarity, and spatially controlled cell division. Here we compared the predictions of a mathematical model of epithelial growth with the morphological analysis of 3D epithelial structures. In both in vitro cyst models and in developing epithelial structures in vivo, epithelial growth could take place close to or far from mechanical equilibrium, and was determined by the hierarchy of time-scales of cell division, cell–cell rearrangements, and lumen dynamics. Equilibrium properties could be inferred by the analysis of cell–cell contact topologies, and the nonequilibrium phenotype was altered by inhibiting ROCK activity. The occurrence of an aberrant multilumen phenotype was linked to fast nonequilibrium growth, even when geometric control of cell division was correctly enforced. We predicted and verified experimentally that slowing down cell division partially rescued a multilumen phenotype induced by altered polarity. These results improve our understanding of the development of epithelial organs and, ultimately, of carcinogenesis. PMID:24145168

Dynamics and control of sister kinetochore behavior during the meiotic divisions in Drosophila spermatocytes

PubMed Central

2018-01-01

Sister kinetochores are connected to the same spindle pole during meiosis I and to opposite poles during meiosis II. The molecular mechanisms controlling the distinct behavior of sister kinetochores during the two meiotic divisions are poorly understood. To study kinetochore behavior during meiosis, we have optimized time lapse imaging with Drosophila spermatocytes, enabling kinetochore tracking with high temporal and spatial resolution through both meiotic divisions. The correct bipolar orientation of chromosomes within the spindle proceeds rapidly during both divisions. Stable bi-orientation of the last chromosome is achieved within ten minutes after the onset of kinetochore-microtubule interactions. Our analyses of mnm and tef mutants, where univalents instead of bivalents are present during meiosis I, indicate that the high efficiency of normal bi-orientation depends on pronounced stabilization of kinetochore attachments to spindle microtubules by the mechanical tension generated by spindle forces upon bi-orientation. Except for occasional brief separation episodes, sister kinetochores are so closely associated that they cannot be resolved individually by light microscopy during meiosis I, interkinesis and at the start of meiosis II. Permanent evident separation of sister kinetochores during M II depends on spindle forces resulting from bi-orientation. In mnm and tef mutants, sister kinetochore separation can be observed already during meiosis I in bi-oriented univalents. Interestingly, however, this sister kinetochore separation is delayed until the metaphase to anaphase transition and depends on the Fzy/Cdc20 activator of the anaphase-promoting complex/cyclosome. We propose that univalent bi-orientation in mnm and tef mutants exposes a release of sister kinetochore conjunction that occurs also during normal meiosis I in preparation for bi-orientation of dyads during meiosis II. PMID:29734336

77 FR 46125 - Sunoco, Inc., R&M, Refining Division, Marcus Hook, PA; Sunoco, Inc., 10 Industrial Hwy., MS4...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-02

... DEPARTMENT OF LABOR Employment and Training Administration [TA-W-81,145; TA-W-81,145A] Sunoco, Inc., R&M, Refining Division, Marcus Hook, PA; Sunoco, Inc., 10 Industrial Hwy., MS4 Building G, Lester, PA; Notice of Negative Determination on Reconsideration On April 30, 2012, the Department of Labor issued an Affirmative Determination Regarding...

77 FR 29362 - Sunoco, Inc., R&M Refining Division, Marcus Hook, PA; Sunoco, Inc., 10 Industrial Hwy, MS4...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-17

... DEPARTMENT OF LABOR Employment and Training Administration [TA-W-81,145; TA-W-81,145A] Sunoco, Inc., R&M Refining Division, Marcus Hook, PA; Sunoco, Inc., 10 Industrial Hwy, MS4 Building G, Lester, PA; Notice of Affirmative Determination Regarding Application for Reconsideration By application dated March 26, 2012, the United Steel Workers Union...

Presentations - Herriott, T.M. and others, 2015 | Alaska Division of

Science.gov Websites

Details Title: Sequence stratigraphic framework of the Upper Jurassic Naknek Formation, Cook Inlet forearc Resident Business in Alaska Visiting Alaska State Employees DGGS State of Alaska search Alaska Division of ., Wartes, M.A., and Decker, P.L., 2015, Sequence stratigraphic framework of the Upper Jurassic Naknek

Focusing light through random scattering media by four-element division algorithm

NASA Astrophysics Data System (ADS)

Fang, Longjie; Zhang, Xicheng; Zuo, Haoyi; Pang, Lin

2018-01-01

The focusing of light through random scattering materials using wavefront shaping is studied in detail. We propose a newfangled approach namely four-element division algorithm to improve the average convergence rate and signal-to-noise ratio of focusing. Using 4096 independently controlled segments of light, the intensity at the target is 72 times enhanced over the original intensity at the same position. The four-element division algorithm and existing phase control algorithms of focusing through scattering media are compared by both of the numerical simulation and the experiment. It is found that four-element division algorithm is particularly advantageous to improve the average convergence rate of focusing.

INFLIGHT (MISSION CONTROL CENTER [MCC]) - STS-2 - JSC

NASA Image and Video Library

1981-11-14

S81-39508 (14 Nov. 1981) --- The successful STS-2 landing at Edwards Air Force Base in California was cause for celebration in the Johnson Space Center?s Mission Control Center shortly before 3:30 p.m. (CST) on Nov. 14, 1981. JSC Director Christopher C. Kraft Jr. (center), enjoys a traditional ?touchdown? cigar. He is flanked by retiring lead engineer Maxime Faget (left) and Thomas L. Moser of the structures and mechanics division, who join the celebration. The second flight of the space shuttle Columbia lasted two days, six hours, 13 minutes and a few seconds. Photo credit: NASA

33 CFR 274.6 - Division/district pest control programs.

Code of Federal Regulations, 2010 CFR

2010-07-01

..., DEPARTMENT OF DEFENSE PEST CONTROL PROGRAM FOR CIVIL WORKS PROJECTS Project Operation § 274.6 Division... from time to time, will be used as guides in selecting the type of chemicals and the method of application in the control of vegetation and pests at civil works projects. (b) Responsibilities and reports...

Metabolism and the Control of Cell Fate Decisions and Stem Cell Renewal

PubMed Central

Ito, Kyoko; Ito, Keisuke

2016-01-01

Although the stem cells of various tissues remain in the quiescent state to maintain their undifferentiated state, they also undergo cell divisions as required, and if necessary, even a single stem cell is able to provide for lifelong tissue homeostasis. Stem cell populations are precisely controlled by the balance between their symmetric and asymmetric divisions, with their division patterns determined by whether the daughter cells involved retain their self-renewal capacities. Recent studies have reported that metabolic pathways and the distribution of mitochondria are regulators of the division balance of stem cells and that metabolic defects can shift division balance toward symmetric commitment, which leads to stem cell exhaustion. It has also been observed that in asymmetric division, old mitochondria, which are central metabolic organelles, are segregated to the daughter cell fated to cell differentiation, whereas in symmetric division, young and old mitochondria are equally distributed between both daughter cells. Thus, metabolism and mitochondrial biology play important roles in stem cell fate decisions. As these decisions directly affect tissue homeostasis, understanding their regulatory mechanisms in the context of cellular metabolism is critical. PMID:27482603

Metabolism and the Control of Cell Fate Decisions and Stem Cell Renewal.

PubMed

Ito, Kyoko; Ito, Keisuke

2016-10-06

Although the stem cells of various tissues remain in the quiescent state to maintain their undifferentiated state, they also undergo cell divisions as required, and if necessary, even a single stem cell is able to provide for lifelong tissue homeostasis. Stem cell populations are precisely controlled by the balance between their symmetric and asymmetric divisions, with their division patterns determined by whether the daughter cells involved retain their self-renewal capacities. Recent studies have reported that metabolic pathways and the distribution of mitochondria are regulators of the division balance of stem cells and that metabolic defects can shift division balance toward symmetric commitment, which leads to stem cell exhaustion. It has also been observed that in asymmetric division, old mitochondria, which are central metabolic organelles, are segregated to the daughter cell fated to cell differentiation, whereas in symmetric division, young and old mitochondria are equally distributed between both daughter cells. Thus, metabolism and mitochondrial biology play important roles in stem cell fate decisions. As these decisions directly affect tissue homeostasis, understanding their regulatory mechanisms in the context of cellular metabolism is critical.

IFLA General Conference, 1991. Division of Collections and Services: Open Forum of Division of Collections and Services; Section of Acquisition and Exchange; Section of Interlending and Document Delivery; Section of Serial Publications; Newspapers; Section of Government Information and Official Publications; Section of Rare [Books] and Manuscripts. Booklet 5.

ERIC Educational Resources Information Center

International Federation of Library Associations and Institutions, The Hague (Netherlands).

The 14 papers in this collection were presented at 6 sections of the Division of Collections and Services: (1) "Open Forum of the Division of Collections and Services Report of the Section on Acquisition and Exchange" (Ulrich Montag); (2) "Acquisition Policy of the USSR National Library Collection" (Z. P. Sorokina and S. M.…

Publications - GMC 400 | Alaska Division of Geological & Geophysical

Science.gov Websites

, Kashwitna Lake #1, Little Su #1, Sheep Creek #1, and Slats #1 coal-bed methane wells Authors: Posey, C.M methane wells: Alaska Division of Geological & Geophysical Surveys Geologic Materials Center Data

Publications - GMC 363 | Alaska Division of Geological & Geophysical

Science.gov Websites

Division of Geological & Geophysical Surveys Comments: Excel files of grain size data and point count . http://doi.org/10.14509/20244 Publication Products Report Report Information gmc363.pdf (6.6 M) Excel

PUBLICATIONS (AIR POLLUTION TECHNOLOGY BRANCH, AIR POLLUTION PREVENTION AND CONTROL DIVISION, NRMRL)

EPA Science Inventory

The Air Pollution Technology Branch (APTB) of NRMRL's Air Pollution Prevention and Control Division produces and publishes highly specialized technical and scientific documents related to APTB's research. Areas of research covered include artificial intelligence, CFC destruction,...

GREENHOUSE GASES (ATMOSPHERIC PROTECTION BRANCH, AIR POLLUTION PREVENTION AND CONTROL DIVISION, NRMRL)

EPA Science Inventory

Greenhouse gas (GHG) emissions are projected for various scenarios and the most appropriate approaches and technologies for mitigation are identified by NRMRL's Air Pollution Prevention and Control Division's Atmospheric Protection Branch (APB). These methods contribute to reduct...

Interaction of a supernova shock with two interstellar clouds

NASA Astrophysics Data System (ADS)

Hansen, J. F.; McKee, C. F.

2005-10-01

The interaction of supernova shocks and interstellar clouds is an important astrophysical phenomenon since it can result in stellar and planetary formation. Our experiments attempt to simulate this mass-loading as it occurs when a shock passes through interstellar clouds. We drive a strong shock using a 5 kJ laser into a foam-filled cylinder with embedded Al spheres (diameter D=120 μm) simulating interstellar clouds. The density ratio between Al and foam is ˜9. We have previously reported on the interaction between shock and a single cloud, and the ensuing Kelvin-Helmholtz and Widnall instabilities. We now report on experiments under way in which two clouds are placed side by side. Cloud separation (center to center) is either 1.2xD or 1.5xD. Initial results for 1.2xD show that cloud material merges and travels further downstream than in the single cloud case. For 1.5xD, material does not merge, but the clouds tilt toward each other. Work performed under the auspices of the Department of Energy by the Lawrence Livermore National Laboratory under contract number W-7405-ENG-48.

Ion Temperature Measurements in an electron beam ion trap (EBIT)

NASA Astrophysics Data System (ADS)

Beiersdorfer, P.; Decaux, V.; Widmann, K.

1997-11-01

An electron beam ion trap consists of a Penning-type cylindrical trap traversed by a high-energy (<= 200 keV), high-density (Ne <= 10^13 cm-3) electron beam. Ions are trapped by the space charge potential of the electron beam, a static potential on the end electrodes, and a 3-T axial magnetic field [1]. The ions are heated by the electron beam and leave the trap once their kinetic energy suffices to overcome the potential barriers. Using high-resolution x-ray spectroscopy, we have made systematic measurements of the temperature of Ti^20+ and Cs^45+ ions in the trap [2]. The dependence of the ion temperature on operating parameters, such as trapping potential, beam current, and neutral gas pressure, will be presented. Temperatures as low as 15.4 ± 4.4 eV and as high as 2 keV were observed. *Work performed under the auspices of the U.S.D.o.E. by Lawrence Livermore National Laboratory under contract No. W-7405-ENG-48. [1] M. Levine et al., Phys. Scripta T22, 157 (1989). [2]P. Beiersdorfer et al., PRL 77, 5356 (1996); P. Beiersdorfer, in AIP Conf. Proc. No. 389, p. 121 (1997).

Oriented cell division: new roles in guiding skin wound repair and regeneration

PubMed Central

Yang, Shaowei; Ma, Kui; Geng, Zhijun; Sun, Xiaoyan; Fu, Xiaobing

2015-01-01

Tissue morphogenesis depends on precise regulation and timely co-ordination of cell division and also on the control of the direction of cell division. Establishment of polarity division axis, correct alignment of the mitotic spindle, segregation of fate determinants equally or unequally between daughter cells, are essential for the realization of oriented cell division. Furthermore, oriented cell division is regulated by intrinsic cues, extrinsic cues and other cues, such as cell geometry and polarity. However, dysregulation of cell division orientation could lead to abnormal tissue development and function. In the present study, we review recent studies on the molecular mechanism of cell division orientation and explain their new roles in skin repair and regeneration. PMID:26582817

Mechanical stretch triggers rapid epithelial cell division through Piezo1.

PubMed

Gudipaty, S A; Lindblom, J; Loftus, P D; Redd, M J; Edes, K; Davey, C F; Krishnegowda, V; Rosenblatt, J

2017-03-02

Despite acting as a barrier for the organs they encase, epithelial cells turn over at some of the fastest rates in the body. However, epithelial cell division must be tightly linked to cell death to preserve barrier function and prevent tumour formation. How does the number of dying cells match those dividing to maintain constant numbers? When epithelial cells become too crowded, they activate the stretch-activated channel Piezo1 to trigger extrusion of cells that later die. However, it is unclear how epithelial cell division is controlled to balance cell death at the steady state. Here we show that mammalian epithelial cell division occurs in regions of low cell density where cells are stretched. By experimentally stretching epithelia, we find that mechanical stretch itself rapidly stimulates cell division through activation of the Piezo1 channel. To stimulate cell division, stretch triggers cells that are paused in early G2 phase to activate calcium-dependent phosphorylation of ERK1/2, thereby activating the cyclin B transcription that is necessary to drive cells into mitosis. Although both epithelial cell division and cell extrusion require Piezo1 at the steady state, the type of mechanical force controls the outcome: stretch induces cell division, whereas crowding induces extrusion. How Piezo1-dependent calcium transients activate two opposing processes may depend on where and how Piezo1 is activated, as it accumulates in different subcellular sites with increasing cell density. In sparse epithelial regions in which cells divide, Piezo1 localizes to the plasma membrane and cytoplasm, whereas in dense regions in which cells extrude, it forms large cytoplasmic aggregates. Because Piezo1 senses both mechanical crowding and stretch, it may act as a homeostatic sensor to control epithelial cell numbers, triggering extrusion and apoptosis in crowded regions and cell division in sparse regions.

Subtractive, divisive and non-monotonic gain control in feedforward nets linearized by noise and delays.

PubMed

Mejias, Jorge F; Payeur, Alexandre; Selin, Erik; Maler, Leonard; Longtin, André

2014-01-01

The control of input-to-output mappings, or gain control, is one of the main strategies used by neural networks for the processing and gating of information. Using a spiking neural network model, we studied the gain control induced by a form of inhibitory feedforward circuitry-also known as "open-loop feedback"-, which has been experimentally observed in a cerebellum-like structure in weakly electric fish. We found, both analytically and numerically, that this network displays three different regimes of gain control: subtractive, divisive, and non-monotonic. Subtractive gain control was obtained when noise is very low in the network. Also, it was possible to change from divisive to non-monotonic gain control by simply modulating the strength of the feedforward inhibition, which may be achieved via long-term synaptic plasticity. The particular case of divisive gain control has been previously observed in vivo in weakly electric fish. These gain control regimes were robust to the presence of temporal delays in the inhibitory feedforward pathway, which were found to linearize the input-to-output mappings (or f-I curves) via a novel variability-increasing mechanism. Our findings highlight the feedforward-induced gain control analyzed here as a highly versatile mechanism of information gating in the brain.

Subtractive, divisive and non-monotonic gain control in feedforward nets linearized by noise and delays

PubMed Central

Mejias, Jorge F.; Payeur, Alexandre; Selin, Erik; Maler, Leonard; Longtin, André

2014-01-01

The control of input-to-output mappings, or gain control, is one of the main strategies used by neural networks for the processing and gating of information. Using a spiking neural network model, we studied the gain control induced by a form of inhibitory feedforward circuitry—also known as “open-loop feedback”—, which has been experimentally observed in a cerebellum-like structure in weakly electric fish. We found, both analytically and numerically, that this network displays three different regimes of gain control: subtractive, divisive, and non-monotonic. Subtractive gain control was obtained when noise is very low in the network. Also, it was possible to change from divisive to non-monotonic gain control by simply modulating the strength of the feedforward inhibition, which may be achieved via long-term synaptic plasticity. The particular case of divisive gain control has been previously observed in vivo in weakly electric fish. These gain control regimes were robust to the presence of temporal delays in the inhibitory feedforward pathway, which were found to linearize the input-to-output mappings (or f-I curves) via a novel variability-increasing mechanism. Our findings highlight the feedforward-induced gain control analyzed here as a highly versatile mechanism of information gating in the brain. PMID:24616694

Military Construction: FY2017 Appropriations

DTIC Science & Technology

2016-10-04

combined the versions of the Transportation, Housing and Urban Development (T-HUD), Military Construction and Veterans Affairs (MILCON/VA), and Zika ...amended bill, and sent it to the House. The House substituted its own amendment in three divisions (Division A: MILCON/VA, Division B: Zika Response...Appropriations, and Division C: Zika Vector Control), removing the T-HUD portion for H.R. 2577, passed the bill, and requested a conference. The

Ultraviolet B and Incidence Rates of Leukemia Worldwide

DTIC Science & Technology

2011-07-01

basics. Gaithersburg MD: Aspen, 2000:141–5. 49. DeanHT, Arnold FA Jr, Jay P, Knutson JW. Studies onmass control of dental caries through fluoridation...Journal of Preventive Medicine.z D m s v t o Introduction Approximately 300,500 new cases and 225,500deaths from leukemia occur annually world-wide.1 In...expo- sure to tobacco smoke,3 electromagnetic fıelds,4 ben- From the Division of Epidemiology, Department of Family and Preventive Medicine, University

Constriction of the mitochondrial inner compartment is a priming event for mitochondrial division

PubMed Central

Cho, Bongki; Cho, Hyo Min; Jo, Youhwa; Kim, Hee Dae; Song, Myungjae; Moon, Cheil; Kim, Hyongbum; Kim, Kyungjin; Sesaki, Hiromi; Rhyu, Im Joo; Kim, Hyun; Sun, Woong

2017-01-01

Mitochondrial division is critical for the maintenance and regulation of mitochondrial function, quality and distribution. This process is controlled by cytosolic actin-based constriction machinery and dynamin-related protein 1 (Drp1) on mitochondrial outer membrane (OMM). Although mitochondrial physiology, including oxidative phosphorylation, is also important for efficient mitochondrial division, morphological alterations of the mitochondrial inner-membrane (IMM) have not been clearly elucidated. Here we report spontaneous and repetitive constriction of mitochondrial inner compartment (CoMIC) associated with subsequent division in neurons. Although CoMIC is potentiated by inhibition of Drp1 and occurs at the potential division spots contacting the endoplasmic reticulum, it appears on IMM independently of OMM. Intra-mitochondrial influx of Ca2+ induces and potentiates CoMIC, and leads to K+-mediated mitochondrial bulging and depolarization. Synergistically, optic atrophy 1 (Opa1) also regulates CoMIC via controlling Mic60-mediated OMM–IMM tethering. Therefore, we propose that CoMIC is a priming event for efficient mitochondrial division. PMID:28598422

Elliptic net and its cryptographic application

NASA Astrophysics Data System (ADS)

Muslim, Norliana; Said, Mohamad Rushdan Md

2017-11-01

Elliptic net is a generalization of elliptic divisibility sequence and in cryptography field, most cryptographic pairings that are based on elliptic curve such as Tate pairing can be improved by applying elliptic nets algorithm. The elliptic net is constructed by using n dimensional array of values in rational number satisfying nonlinear recurrence relations that arise from elliptic divisibility sequences. The two main properties hold in the recurrence relations are for all positive integers m>n, hm +nhm -n=hm +1hm -1hn2-hn +1hn -1hm2 and hn divides hm whenever n divides m. In this research, we discuss elliptic divisibility sequence associated with elliptic nets based on cryptographic perspective and its possible research direction.

Management implications of brood division in Golden-winged Warblers: Chapter 10

USGS Publications Warehouse

Peterson, Sean M.; Streby, Henry M.; Andersen, David E.

2016-01-01

Brood division in the postfledging period is a common avian behavior that is not well understood. Brood division has been reported in Golden-winged Warblers (Vermivora chrysoptera), but it is not known how common this behavior is, whether males and females exhibit different strategies related to parental care and habitat use, or how brood division might influence management strategies. We radiomarked fledglings and monitored divided broods of Golden-winged Warblers from fledging until independence from parental care at three sites in the western Great Lakes region from 2010 to 2012 to assess differences in strategies between male and female parents and to consider possible management implications. Male - and female-reared sub-broods exhibited different space use during the dependent post-fledging period despite similar fledgling survivial, cover-type use, and microhabitat use. By independence, female-reared sub-broods traveled over twice as far from the nest (mean = 461 ± 81) SE m) as male-reared sub-broods (164 ± 41 m). Additionally, female-reared sub-broods traveled over three times as far from the natal patch edge (35 ± 72 m) as male-reared sub-broods (108 ± 36 m). Without accounting for differential space use by male- and female-reared sub-broods, we would have reported broods traveling 292 (± 46 m) from the nest and 214 (± 40m) from the natal patch edge - distances that do not reflect how far females move sub-broods. Parental strategies differ between sexes with regard to movement patterns, and we recommend incorporating the differences in space use between sexes in future management plans for Golden-winged Warblers  and other species that employ brood division. Specifically, management actions might be most effective when they are applied at spatial scales large enough to incorporate the habitat requirements of both sexes throughout the entire reproductive season. 

Tri-channel single-mode terahertz quantum cascade laser.

PubMed

Wang, Tao; Liu, Jun-Qi; Liu, Feng-Qi; Wang, Li-Jun; Zhang, Jin-Chuan; Wang, Zhan-Guo

2014-12-01

We report on a compact THz quantum cascade laser source emitting at, individually controllable, three different wavelengths (92.6, 93.9, and 95.1 μm). This multiwavelength laser array can be used as a prototype of the emission source of THz wavelength division multiplex (WDM) wireless communication system. The source consists of three tapered single-mode distributed feedback (DFB) terahertz quantum cascade lasers fabricated monolithically on a single chip. All array elements feature longitudinal as well as lateral single-mode in the entire injection range. The peak output powers of individual lasers are 42, 73, and 37 mW at 10 K, respectively.

A single-cell pedigree analysis of alternative stochastic lymphocyte fates

PubMed Central

Hawkins, E. D.; Markham, J. F.; McGuinness, L. P.; Hodgkin, P. D.

2009-01-01

In contrast to most stimulated lymphocytes, B cells exposed to Toll-like receptor 9 ligands are nonself-adherent, allowing individual cells and families to be followed in vitro for up to 5 days. These B cells undergo phases typical of an adaptive response, dividing up to 6 times before losing the impetus for further growth and division and eventually dying by apoptosis. Using long-term microscopic imaging, accurate histories of individual lymphocyte fates were collected. Quantitative analysis of family relationships revealed that times to divide of siblings were strongly related but these correlations were progressively lost through consecutive divisions. A weaker, but significant, correlation was also found for death times among siblings. Division cessation is characterized by a loss of cell growth and the division in which this occurs is strongly inherited from the original founder cell and is related to the size this cell reaches before its first division. Thus, simple division-based dilution of factors synthesized during the first division may control the maximum division reached by stimulated cells. The stochastic distributions of times to divide, times to die, and divisions reached are also measured. Together, these results highlight the internal cellular mechanisms that control immune responses and provide a foundation for the development of new mathematical models that are correct at both single-cell and population levels. PMID:19633185

Expression of genomic AtCYCD2;1 in Arabidopsis induces cell division at smaller cell sizes: implications for the control of plant growth.

PubMed

Qi, Ruhu; John, Peter Crook Lloyd

2007-07-01

The Arabidopsis (Arabidopsis thaliana) CYCD2;1 gene introduced in genomic form increased cell formation in the Arabidopsis root apex and leaf, while generating full-length mRNA, raised CDK/CYCLIN enzyme activity, reduced G1-phase duration, and reduced size of cells at S phase and division. Other cell cycle genes, CDKA;1, CYCLIN B;1, and the cDNA form of CYCD2;1 that produced an aberrantly spliced mRNA, produced smaller or zero increases in CDK/CYCLIN activity and did not increase the number of cells formed. Plants with a homozygous single insert of genomic CYCD2;1 grew with normal morphology and without accelerated growth of root or shoot, not providing evidence that cell formation or CYCLIN D2 controls growth of postembryonic vegetative tissues. At the root apex, cells progressed normally from meristem to elongation, but their smaller size enclosed less growth and a 40% reduction in final size of epidermal and cortical cells was seen. Smaller elongated cell size inhibited endoreduplication, indicating a cell size requirement. Leaf cells were also smaller and more numerous during proliferation and epidermal pavement and palisade cells attained 59% and 69% of controls, whereas laminas reached normal size. Autonomous control of expansion was therefore not evident in abundant cell types that formed tissues of root or leaf. Cell size was reduced by a greater number formed in a tissue prior to cell and tissue expansion. Initiation and termination of expansion did not correlate with cell dimension or number and may be determined by tissue-wide signals acting across cellular boundaries.

1997 JOURNAL ARTICLES FROM LRPCD (TREATMENT AND DESTRUCTION BRANCH, LAND REMEDIATION AND POLLUTION CONTROL DIVISION, NRMRL)

EPA Science Inventory

The Treatment and Destruction Branch (TDB) of NRMRL's Land Remediation and Pollution Control Division (LRPCD) produces and publishes highly technical and scientific documents relating to TDB's research. TDB conducts bioremediation and physical/chemical treatment research. The res...

1996 JOURNAL ARTICLES FROM LRPCD (TREATMENT AND DESTRUCTION BRANCH, LAND REMEDIATION AND POLLUTION CONTROL DIVISION, NRMRL)

EPA Science Inventory

The Treatment and Destruction Branch (TDB) of NRMRL's Land Remediation and Pollution Control Division (LRPCD) produces and publishes highly technical and scientific articles relating to TDB's research. TDB conducts bioremediation and physical/chemical treatment research. The rese...

2000 JOURNAL ARTICLES FROM LRPCD (TREATMENT AND DESTRUCTION BRANCH, LAND REMEDIATION AND POLLUTION CONTROL DIVISION, NRMRL)

EPA Science Inventory

The Treatment and Destruction Branch (TDB) of NRMRL's Land Remediation and Pollution Control Division (LRPCD)produces and publishes highly specialized technical and scientific documents relating to TDB's research. TDB conducts bioremediation and physical/chemical treatment resear...

1998 JOURNAL ARTICLES FROM LRPCD (TREATMENT AND DESTRUCTION BRANCH, LAND REMEDIATION AND POLLUTION CONTROL DIVISION, NRMRL)

EPA Science Inventory

The Treatment and Destruction Branch (TDB) of NRMRL's Land Remediation and Pollution Control Division (LRPCD) produces and publishes highly technical and scientific documents relating to TDB's research. TDB conducts bioremediation and physical/chemical treatment research. The res...

1999 JOURNAL ARTICLES FROM LRPCD (TREATMENT AND DESTRUCTION BRANCH, LAND REMEDIATION AND POLLUTION CONTROL DIVISION, NRMRL)

EPA Science Inventory

The Treatment and Destruction Branch (TDB) of NRMRL's Land Remediation and Pollution Control Division (LRPCD) produces and publishes highly technical and scientific documents relating to TDB's research. TDB conducts bioremediation and physical/chemical treatment research. The res...

Free-standing oxide superconducting articles

DOEpatents

Wu, Xin D.; Muenchausen, Ross E.

1993-01-01

A substrate-free, free-standing epitaxially oriented superconductive film including a layer of a template material and a layer of a ceramic superconducting material is provided together with a method of making such a substrate-free ceramic superconductive film by coating an etchable material with a template layer, coating the template layer with a layer of a ceramic superconductive material, coating the layer of ceramic superconductive material with a protective material, removing the etchable material by an appropriate means so that the etchable material is separated from a composite structure including the template lay This invention is the result of a contract with the Department of Energy (Contract No. W-7405-ENG-36).

Clamshell tomograph

DOEpatents

Derenzo, Stephen E.; Budinger, Thomas F.

1984-01-01

In brief, the invention is a tomograph modified to be in a clamshell configuration so that the ring or rings may be moved to multiple sampling positions. The tomograph includes an array of detectors arranged in successive adjacent relative locations along a closed curve in a first position in a selected plane, and means for securing the detectors in the relative locations in a first sampling position. The securing means is movable in the plane in two sections and pivotable at one p The U.S. Government has rights in this invention pursuant to Contract No. W-7405-ENG-48 between the U.S. Department of Energy and the University of California.

THE IMPORTANCE OF A SUCCESSFUL QUALITY ASSURANCE (QA) PROGRAM FROM A RESEARCH MANAGER'S PERSPECTIVE

EPA Science Inventory

The paper discusses the Air Pollution Prevention and Control Division's Quality Assurance (QA) program and the approaches used to meet QA requirements in the Division. The presentation is a technical manager's perspective of the Division's requirements for and approach to QA in i...

Body Size Changes Among National Collegiate Athletic Association New England Division III Football Players, 1956-2014: Comparison With Age-Matched Population Controls.

PubMed

Elliott, Kayla R; Harmatz, Jerold S; Zhao, Yanli; Greenblatt, David J

2016-05-01

Collegiate football programs encourage athletes to pursue high body weights. To examine position-dependent trends over time in body size characteristics among football players in the National Collegiate Athletic Association Division III New England Small College Athletic Conference (NESCAC) from 1956 to 2014 and to compare the observed absolute and relative changes with those in age-matched male population controls. Descriptive laboratory study. Medical school affiliated with a NESCAC institution. Football team rosters from the 10-member NESCAC schools, available as public documents, were analyzed along with body size data from general population males aged 20 to 29 years from the National Health and Nutrition Examination Survey (NHANES). Body weight, height, and calculated body mass index were evaluated using analysis of variance, linear regression, and nonlinear regression to determine the distribution features of size variables and changes associated with time (year), school, and position. Among NESCAC linemen, absolute and relative changes over time in body weight and body mass index exceeded corresponding changes in the NHANES population controls. New England Small College Athletic Conference offensive linemen body weights increased by 37.5% from 1956 to 2014 (192 to 264 lb [86.4 to 118.8 kg]), compared with a 12% increase (164 to 184 lb [73.8 to 82.8 kg]) since 1961 in the NHANES population controls. Body mass index changed in parallel with body weight and exceeded 35 kg/m(2) in more than 30% of contemporary NESCAC offensive linemen. Among skill players in the NESCAC group, time-related changes in body size characteristics generally paralleled those in the NHANES controls. High body weight and body mass indices were evident in offensive linemen, even among those in Division III football programs with no athletic scholarships. These characteristics may be associated with adverse cardiovascular and metabolic outcomes. We need approaches to encourage risk modification in the postfootball lifestyles of these individuals.

New Cancer Prevention and Control Central Institutional Review Board Established | Division of Cancer Prevention

Cancer.gov

The NCI Central Institutional Review Board (CIRB) Initiative announced the establishment of the Cancer Prevention and Control (CPC) CIRB January 14, extending the benefits of centralized review to investigators participating in clinical trials sponsored by the Division of Cancer Prevention (DCP). |

Attitude and knowledge changes in collegiate dancers following a short-term, team-centered prevention program on eating disorders.

PubMed

Torres-McGehee, Toni M; Green, James M; Leaver-Dunn, Deidre; Leeper, James D; Bishop, Phillip A; Richardson, Mark T

2011-06-01

Eating knowledge, nutritional knowledge, and psychological changes among female collegiate dancers were examined before and after a 4-wk. team-centered program on sport nutrition, exercise, and disordered eating consequences. Collegiate female dancers from two NCAA Division I institutions participated in a control (n = 19; M age = 19.1 yr., SD = 1.0) or intervention (n = 21; M age = 19.2 yr., SD = 1.2) group. Measures were administered to both groups before and after intervention to assess eating disorders, depression, and nutritional and disordered eating knowledge. There was a statistically significant increase in scores on nutritional and overall eating disorder knowledge in the intervention group compared to the control group. Mean scores on depression, drive for thinness, body dissatisfaction, and maturity fears decreased in the intervention group.

Clark AFB, Philippines. Revised Uniform Summary of Surface Weather Observations (RUSSWO). Parts A-F.

DTIC Science & Technology

1984-02-01

DATA PROCESSING DIVISION USAFETAC Air Weather Service ( MAC) 2L._qi( .3 I-H M3,; #983270 :.15 11 E 120 33 EL --V478 FT ?.p1K I-ARTS A-F HUMS...THIS PAGE .a.. E ...d) REPORT DOCUMENTATION PAGE READ INSTRUCTIONS REPORTDOCUMENTATIONPAGE_ BEFORE COMPLETING FORM I REPORT NUMBER 12 GOVT ACCESSION NO. 3...62225 P. 320 14 MONITORING AGENCY NAME & ADDRESS(f dlle’. e , Ifr Controlling Offfe) 15. SECURITY CLASS. (of this report) UNCLASSIFIED T5

40 CFR 52.2239 - Original Identification of plan section.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Original Identification of plan section. (a) This section identifies the original “Tennessee Air Pollution... on February 3, 1972, by the Division of Air Pollution Control of the Tennessee Department of Public... Division of Air Pollution Control of the Tennessee Department of Public Health. (3) Statements of intent...

2x2 MIMO-OFDM Gigabit fiber-wireless access system based on polarization division multiplexed WDM-PON.

PubMed

Deng, Lei; Pang, Xiaodan; Zhao, Ying; Othman, M B; Jensen, Jesper Bevensee; Zibar, Darko; Yu, Xianbin; Liu, Deming; Monroy, Idelfonso Tafur

2012-02-13

We propose a spectral efficient radio over wavelength division multiplexed passive optical network (WDM-PON) system by combining optical polarization division multiplexing (PDM) and wireless multiple input multiple output (MIMO) spatial multiplexing techniques. In our experiment, a training-based zero forcing (ZF) channel estimation algorithm is designed to compensate the polarization rotation and wireless multipath fading. A 797 Mb/s net data rate QPSK-OFDM signal with error free (<1 × 10(5)) performance and a 1.59 Gb/s net data rate 16QAM-OFDM signal with BER performance of 1.2 × 10(2) are achieved after transmission of 22.8 km single mode fiber followed by 3 m and 1 m air distances, respectively.

INFLIGHT (MISSION CONTROL CENTER [MCC]) - STS-2 - JSC

NASA Image and Video Library

1981-11-14

S81-39511 (14 Nov. 1981) --- The successful STS-2 landing at Edwards Air Force Base in California was cause for celebration in the Johnson Space Center?s Mission Control Center shortly before 3:30 p.m. (CST) on Nov. 14, 1981. JSC Director Christopher C. Kraft Jr. (center), not only applauds but enjoys a traditional ?touchdown? cigar, as well. Eugene F. Kranz (left), deputy director of flight operations at JSC, and Thomas L. Moser of the structures and mechanics division join the celebration. The second flight of the space shuttle Columbia lasted two days, six hours, 13 minutes and a few seconds. Photo credit: NASA

Role of asymmetric cell division in lifespan control in Saccharomyces cerevisiae

PubMed Central

Higuchi-Sanabria, Ryo; Pernice, Wolfgang M A; Vevea, Jason D; Alessi Wolken, Dana M; Boldogh, Istvan R; Pon, Liza A

2014-01-01

Aging determinants are asymmetrically distributed during cell division in S. cerevisiae, which leads to production of an immaculate, age-free daughter cell. During this process, damaged components are sequestered and retained in the mother cell, and higher functioning organelles and rejuvenating factors are transported to and/or enriched in the bud. Here, we will describe the key quality control mechanisms in budding yeast that contribute to asymmetric cell division of aging determinants including mitochondria, endoplasmic reticulum (ER), vacuoles, extrachromosomal rDNA circles (ERCs), and protein aggregates. PMID:25263578

Control of proliferation and cancer growth by the Hippo signaling pathway

PubMed Central

Ehmer, Ursula; Sage, Julien

2015-01-01

The control of cell division is essential for normal development and the maintenance of cellular homeostasis. Abnormal cell proliferation is associated with multiple pathological states, including cancer. While the Hippo/YAP signaling pathway was initially thought to control organ size and growth, increasing evidence indicates that this pathway also plays a major role in the control of proliferation independent of organ size control. In particular, accumulating evidence indicates that the Hippo/YAP signaling pathway functionally interacts with multiple other cellular pathways and serves as a central node in the regulation of cell division, especially in cancer cells. Here recent observations are highlighted that connect Hippo/YAP signaling to transcription, the basic cell cycle machinery, and the control of cell division. Furthermore, the oncogenic and tumor suppressive attributes of YAP/TAZ are reviewed which emphasizes the relevance of the Hippo pathway in cancer. PMID:26432795

The Division of Labor in Lesbian, Gay, and Heterosexual New Adoptive Parents

ERIC Educational Resources Information Center

Goldberg, Abbie E.; Smith, JuliAnna Z.; Perry-Jenkins, Maureen

2012-01-01

Little research has investigated the division of child care and housework in adoptive or lesbian/gay parent families, yet these contexts "control for" family characteristics such as biological relatedness and parental gender differences known to be linked to family work. This study examined predictors (measured preadoption) of the division of…

A new class of cyclin dependent kinase in Chlamydomonas is required for coupling cell size to cell division

PubMed Central

Li, Yubing; Liu, Dianyi; López-Paz, Cristina; Olson, Bradley JSC; Umen, James G

2016-01-01

Proliferating cells actively control their size by mechanisms that are poorly understood. The unicellular green alga Chlamydomonas reinhardtii divides by multiple fission, wherein a ‘counting’ mechanism couples mother cell-size to cell division number allowing production of uniform-sized daughters. We identified a sizer protein, CDKG1, that acts through the retinoblastoma (RB) tumor suppressor pathway as a D-cyclin-dependent RB kinase to regulate mitotic counting. Loss of CDKG1 leads to fewer mitotic divisions and large daughters, while mis-expression of CDKG1 causes supernumerous mitotic divisions and small daughters. The concentration of nuclear-localized CDKG1 in pre-mitotic cells is set by mother cell size, and its progressive dilution and degradation with each round of cell division may provide a link between mother cell-size and mitotic division number. Cell-size-dependent accumulation of limiting cell cycle regulators such as CDKG1 is a potentially general mechanism for size control. DOI: http://dx.doi.org/10.7554/eLife.10767.001 PMID:27015111

Alternative Ways to Think about Cellular Internal Ribosome Entry*

PubMed Central

Gilbert, Wendy V.

2010-01-01

Internal ribosome entry sites (IRESs) are specialized mRNA elements that allow recruitment of eukaryotic ribosomes to naturally uncapped mRNAs or to capped mRNAs under conditions in which cap-dependent translation is inhibited. Putative cellular IRESs have been proposed to play crucial roles in stress responses, development, apoptosis, cell cycle control, and neuronal function. However, most of the evidence for cellular IRES activity rests on bicistronic reporter assays, the reliability of which has been questioned. Here, the mechanisms underlying cap-independent translation of cellular mRNAs and the contributions of such translation to cellular protein synthesis are discussed. I suggest that the division of cellular mRNAs into mutually exclusive categories of “cap-dependent” and “IRES-dependent” should be reconsidered and that the implications of cellular IRES activity need to be incorporated into our models of cap-dependent initiation. PMID:20576611

Bisphenol A alters oocyte maturation by prematurely closing gap junctions in the cumulus cell-oocyte complex.

PubMed

Acuña-Hernández, Deyanira Guadalupe; Arreola-Mendoza, Laura; Santacruz-Márquez, Ramsés; García-Zepeda, Sihomara Patricia; Parra-Forero, Lyda Yuliana; Olivares-Reyes, Jesús Alberto; Hernández-Ochoa, Isabel

2018-04-01

In ovarian follicles, cumulus cells communicate with the oocyte through gap junction intercellular communication (GJIC), to nurture the oocyte and control its meiosis arrest and division. Bisphenol A (BPA) is a monomer found in polycarbonate-made containers that can induce functional alterations, including impaired oocyte meiotic division and reduced molecule transfer in GJIC. However, how BPA alters oocyte meiotic division is unclear. We investigated whether BPA effects on oocyte meiotic division were correlated with reduced transfer in GJIC. Cumulus cell-oocyte complexes (COCs) isolated from mouse preovulatory follicles were cultured with 0, 0.22, 2.2, 22, 220, and 2200 nM BPA for 2 h. An additional 16-h incubation with epidermal growth factor (EGF) was performed to promote the occurrence of meiotic resumption and progression to metaphase II. Without EGF stimulus, BPA treatment increased the percentage of oocytes undergoing meiotic resumption, decreased GJIC in the COCs, and did not modify GJIC gene (Cx43 and Cx37) and protein (CX43) expression. Following EGF stimulus, BPA increased the percentage of oocytes that remained at the anaphase and telophase stages, and decreased the percentage of oocytes reaching the metaphase II stage. Concomitantly, BPA reduced the expansion of cumulus cells. Carbenoxolone (a GJIC inhibitor) and 6-diazo-5-oxo-l-norleucine (a cumulus cell-expansion inhibitor) exerted effects on meiotic division similar to those exerted by BPA. These data suggest that BPA accelerates meiotic progression, leading to impaired prophase I-to-metaphase II transition, and that this adverse effect is correlated with reduced bidirectional communication in the COC. Copyright © 2018 Elsevier Inc. All rights reserved.

Measurement And Modeling Of Fe VIII To Fe XVI M-shell Emission In The Extreme Ultraviolet

NASA Astrophysics Data System (ADS)

Beiersdorfer, Peter; Lepson, J. K.; Hurwitz, M.

2007-05-01

The solar EUV emission near 200 Å is presently being studied with high resolution with the Cosmic Hot Interstellar Plasma Spectrometer (CHIPS), which focuses on the emission between 90 and 270 Å, and with the EUV Imaging Spectrometer on Hinode, which focuses on the region 180 to 204 Å and 250 to 290 Å. The Solar EUV Experiment on the TIMED spacecraft also observes this spectral band but with greatly reduced resolution. The spectrum in this region is dominated by emission from moderate charge states of iron. The interpretation of the data relies on accurate and complete plasma emission models, notably CHIANTI. We have performed a series of laboratory measurements of the 3-3 emission from M-shell iron ions. The measurements cover the range 170 - 250 Å and are made at an electron density of about 1011 cm-3. Emission from Fe VIII through Fe XVI has been identified. Excellent agreement with CHIANTI predictions is found. A few weak transitions are noted in the laboratory data that are predicted by CHIANTI to be vanishingly small and should not have been observed. These are tentatively attributed to transitions in Fe XV. A comparison with observations from CHIPS is also presented. This work was supported in part by NASA's Solar and Heliospheric Physics Supporting Research and Technology Program. Work at UC-LLNL was performed under the auspices of the DOE by under Contract W-7405-Eng-48.

Effects of Ion-ion Collisions and Inhomogeneity in Two-dimensional Simulations of Stimulated Brillouin Backscattering*

NASA Astrophysics Data System (ADS)

Cohen, B. I.

2005-10-01

Two-dimensional simulations of stimulated Brillouin backscattering (SBBS) with the BZOHAR^1 code have been extended to include ion-ion collisions and spatial nonuniformity in the mean ion flow. BZOHAR hybrid simulations (particle-in-cell kinetic ions and Boltzmann fluid electrons) have shown^2 that SBBS saturation is dominated by ion trapping effects and secondary instability of the primary ion wave (decay into subharmonic ion waves and ion quasi-modes). Here we address the effects of ion collisions^3 on SBBS saturation and employ the efficient Langevin ion collision algorithm of Ref. 4 and the Fokker-Planck collision operator of Ref. 5. We also report simulations of SBBS with a linear gradient in the mean ion drift, which in conjunction with the nonlinear frequency shift due to ion trapping can introduce auto-resonance effects that may enhance reflectivities.^6 For SBBS in a high-gain limit with ion collisions or inhomogeneity, we find that ion trapping and secondary ion wave instabilities are robust saturation mechanisms. *Work performed for US DOE by UC LLNL under Contr. W-7405-ENG-48. ^1B.I. Cohen, et al., Phys. Plasmas 4, 956 (1997). ^2B.I. Cohen, et al., Phys. Plasmas, 12, 052703 (2005),. ^ 3P.W. Rambo, et al., Phys. Rev. Lett. 79, 83 (1997). ^ 4M.E. Jones, et al., J. Comp. Phys. 123, 169, (1996). ^ 5W. M. Manheimer, et al., J. Comp. Phys. 138, 563 (1997). ^ 6E.A. Williams, et al., Phys. Plasmas 11, 231 (2004).

Audit Oversight: Quality Control System at U.S. Special Operations Command Inspector General Audit Division

DTIC Science & Technology

2002-08-21

The Audit Division provides the Commander, U.S. Special Operations Command (USSOCOM) with professional auditing services to safeguard, account for...and ensure the proper use of special operations forces assets in accomplishing the USSOCOM mission. The Audit Division reports to the USSOCOM Inspector...U.S. Army Special Operations Command, Naval Special Warfare Command, and the Joint Special Operations Command. Appendix A contains a summary of the Audit Division policy and procedures.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

This report contains the following information from the Instrumentation and Controls Division of Oak Ridge National Laboratory: supplementary activities; seminars; publications and presentations; scientific and professional activities, achievements, and awards; and division organization charts.

75 FR 41895 - Whirlpool Corporation, Evansville Division, Including On-Site Leased Workers From Andrews...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-19

... DEPARTMENT OF LABOR Employment and Training Administration [TA-W-72,585] Whirlpool Corporation, Evansville Division, Including On-Site Leased Workers From Andrews International, Inc., M.H. Equipment, and Kenco Logistics Services, LLC, Evansville, IN; Amended Certification Regarding Eligibility To Apply for...

Meiosis Drives Extraordinary Genome Plasticity in the Haploid Fungal Plant Pathogen Mycosphaerella Graminicola

USDA-ARS?s Scientific Manuscript database

Meiosis in the plant-pathogenic fungus Mycosphaerella graminicola results in eight ascospores due to a mitotic division following the two meiotic divisions. The transient diploid phase allows for recombination among homologous chromosomes. However, some chromosomes of M. graminicola lack homologs an...

High Energy Density Dielectrics for Pulsed Power Applications

DTIC Science & Technology

2008-09-01

Energy/Power/Thermal Division //Signature// DEREK M. LINCOLN, Major, USAF Deputy Chief Energy/Power/Thermal Division Propulsion Directorate...comparative study," J. Vac. Sci. Technol. A 16, (1998), 2804-2815. 11 T.T. Leung and C.W. Ong . “Nearly amorphous to epitxial growth of aluminum nitride

Groundwater Technical Support Center (GWTSC) Annual Report Fiscal Year 2016 (FY16)

EPA Science Inventory

The Groundwater Technical Support Center (GWTSC) is part of the Groundwater, Watershed, and Ecosystem Restoration Division (GWERD), which is based in the Robert S. Kerr Environmental Research Center in Ada, Oklahoma. The GWERD is a research division of EPA’s National Risk M...

MinD and MinE Interact with Anionic Phospholipids and Regulate Division Plane Formation in Escherichia coli*

PubMed Central

Renner, Lars D.; Weibel, Douglas B.

2012-01-01

The Min proteins (MinC, MinD, and MinE) form a pole-to-pole oscillator that controls the spatial assembly of the division machinery in Escherichia coli cells. Previous studies identified that interactions of MinD with phospholipids positioned the Min machinery at the membrane. We extend these studies by measuring the affinity, kinetics, and ATPase activity of E. coli MinD, MinE, and MinDE binding to supported lipid bilayers containing varying compositions of anionic phospholipids. Using quartz crystal microbalance measurements, we found that the binding affinity (Kd) for the interaction of recombinant E. coli MinD and MinE with lipid bilayers increased with increasing concentration of the anionic phospholipids phosphatidylglycerol and cardiolipin. The Kd for MinD (1.8 μm) in the presence of ATP was smaller than for MinE (12.1 μm) binding to membranes consisting of 95:5 phosphatidylcholine/cardiolipin. The simultaneous binding of MinD and MinE to membranes revealed that increasing the concentration of anionic phospholipid stimulates the initial rate of adsorption (kon). The ATPase activity of MinD decreased in the presence of anionic phospholipids. These results indicate that anionic lipids, which are concentrated at the poles, increase the retention of MinD and MinE and explain its dwell time at this region of bacterial cells. These studies provide insight into interactions between MinD and MinE and between these proteins and membranes that are relevant to understanding the process of bacterial cell division, in which the interaction of proteins and membranes is essential. PMID:23012351

Responsibility for Teaching Pain Control in U.S. Dental Schools.

ERIC Educational Resources Information Center

Smith, Peter B.; Campbell, Robert L.

1993-01-01

A national survey of 53 dental schools found most were not interested in developing a separate division or department of dental anesthesiology. Of those with a dentist anesthesiologist responsible for teaching pain control, all have or favor such a division. Less than one-third employ professionals limiting their practice to anesthesiology. (MSE)

Instrumentation and Controls Division Progress Report for the Period of July 1, 1994 to December 31, 1997: Publications, Presentations, Activities, and Awards

DOE Office of Scientific and Technical Information (OSTI.GOV)

McDonald, D.W.

This report contains a record of publishing and other activities in the Oak Ridge National Laboratory (ORNL) Instrumentation and Controls (I&C) Division for the period of July 1, 1994, to December31, 1997. It is a companion volume to Working Together on New Horizons: Instrumentation and Controls Division Progress Report for the Period of July 1, 1994, to December 31, 1997 (OR.NLA4-6530). Working Together on New Horizons contains illustrated summaries of some of the projects under way in I&C Division. Both books can be obtained by contacting C. R. Brittain (brittain@ornl. gov), P.O. Box 2008, Oak Ridge, TN 37831-6005. l&C Divisionmore » Mission and Vision I&C Division develops and maintains techniques, instruments, and systems that lead to a better understanding of nature and harnessing of natural phenomena for the benefit of humankind. We have dedicated ourselves to accelerating the advancement of science and the transfer of those advancements into products and processes that benefit U.S. industry and enhance the security of our citizens.« less

High variability of facial muscle innervation by facial nerve branches: A prospective electrostimulation study.

PubMed

Raslan, Ashraf; Volk, Gerd Fabian; Möller, Martin; Stark, Vincent; Eckhardt, Nikolas; Guntinas-Lichius, Orlando

2017-06-01

To examine by intraoperative electric stimulation which peripheral facial nerve (FN) branches are functionally connected to which facial muscle functions. Single-center prospective clinical study. Seven patients whose peripheral FN branching was exposed during parotidectomy under FN monitoring received a systematic electrostimulation of each branch starting with 0.1 mA and stepwise increase to 2 mA with a frequency of 3 Hz. The electrostimulation and the facial and neck movements were video recorded simultaneously and evaluated independently by two investigators. A uniform functional allocation of specific peripheral FN branches to a specific mimic movement was not possible. Stimulation of the whole spectrum of branches of the temporofacial division could lead to eye closure (orbicularis oculi muscle function). Stimulation of the spectrum of nerve branches of the cervicofacial division could lead to reactions in the midface (nasal and zygomatic muscles) as well as around the mouth (orbicularis oris and depressor anguli oris muscle function). Frontal and eye region were exclusively supplied by the temporofacial division. The region of the mouth and the neck was exclusively supplied by the cervicofacial division. Nose and zygomatic region were mainly supplied by the temporofacial division, but some patients had also nerve branches of the cervicofacial division functionally supplying the nasal and zygomatic region. FN branches distal to temporofacial and cervicofacial division are not necessarily covered by common facial nerve monitoring. Future bionic devices will need a patient-specific evaluation to stimulate the correct peripheral nerve branches to trigger distinct muscle functions. 4 Laryngoscope, 127:1288-1295, 2017. © 2016 The American Laryngological, Rhinological and Otological Society, Inc.

Protein Kinases and Phosphatases in the Control of Cell Fate

PubMed Central

Bononi, Angela; Agnoletto, Chiara; De Marchi, Elena; Marchi, Saverio; Patergnani, Simone; Bonora, Massimo; Giorgi, Carlotta; Missiroli, Sonia; Poletti, Federica; Rimessi, Alessandro; Pinton, Paolo

2011-01-01

Protein phosphorylation controls many aspects of cell fate and is often deregulated in pathological conditions. Several recent findings have provided an intriguing insight into the spatial regulation of protein phosphorylation across different subcellular compartments and how this can be finely orchestrated by specific kinases and phosphatases. In this review, the focus will be placed on (i) the phosphoinositide 3-kinase (PI3K) pathway, specifically on the kinases Akt and mTOR and on the phosphatases PP2a and PTEN, and on (ii) the PKC family of serine/threonine kinases. We will look at general aspects of cell physiology controlled by these kinases and phosphatases, highlighting the signalling pathways that drive cell division, proliferation, and apoptosis. PMID:21904669

Program for the Division of Chemical Education: Chicago, March 25 29, 2007

NASA Astrophysics Data System (ADS)

Middlecamp, Catherine H.; Bodner, George M.; Jones, Wayne E., Jr.

2007-03-01

Program for the Division of Chemical Education March 2007 meeting in Chicago. All CHED technical sessions including the High School Program will be held in the McCormick Place Convention Complex North, 2301 South Lake Shore Drive. Exceptions are the Undergraduate Program (in the Westin Hotel Michigan Avenue), the Undergraduate Research Poster Sessions (in the Sheraton Chicago Hotel), and any evening programs. Unless otherwise noted, morning sessions begin at 8:30 a.m., afternoon sessions at 1:30 p.m.

Joy Osborne, MS, MPA | Division of Cancer Prevention

Cancer.gov

Joy Osborne is the ARC Director for the Division of Cancer Prevention and the Division of Cancer Control and Population Sciences. The ARC (Administrative Resource Center) provides services to DCP in the areas of budget, contracts, grants, human resources, travel, space and facilities, and other administrative areas. Joy came to NCI in 1992 as a Presidential Management Intern

40 CFR 1065.307 - Linearity verification.

Code of Federal Regulations, 2010 CFR

2010-07-01

... different flow rates. Use a gravimetric reference measurement (such as a scale, balance, or mass comparator... the gas-division system to divide the span gas with purified air or nitrogen. Select gas divisions... PM balance, m max refers to the typical mass of a PM filter. (ii) For linearity verification of...

Publications - MP 126 | Alaska Division of Geological & Geophysical Surveys

Science.gov Websites

DGGS MP 126 Publication Details Title: 400 MHz ground-penetrating radar, Itkillik River, North Slope , Itkillik River, North Slope, Alaska: Alaska Division of Geological & Geophysical Surveys Miscellaneous , North Slope, Alaska, scale 1:100 (8.6 M) Keywords Fluvial; Ground-Penetrating Radar; Itkillik River

PHARMACOKINETIC EVALUATION OF PERFLUOROOCTANOIC ACID IN THE MOUSE

EPA Science Inventory

Pharmacokinetic evaluation of perfluorooctanoic acid in the mouse.

1C. Lau, 2M.J. Strynar, 2A.B. Lindstrom, 1R.G. Hanson, 1J.R. Thibodeaux and 3H.A. Barton.

1Reproductive Toxicology Division, 3Experimental Toxicology Division, NHEERL, 2Human Exposure and Atmospheric...

Publications - MP 8 | Alaska Division of Geological & Geophysical Surveys

Science.gov Websites

MAPTEACH Tsunami Inundation Mapping Energy Resources Gas Hydrates STATEMAP Program information Geologic DGGS MP 8 Publication Details Title: Geothermal resources of Alaska Authors: Motyka, R.J., Moorman, M.A , S.A., 1983, Geothermal resources of Alaska: Alaska Division of Geological & Geophysical Surveys

Publications - GMC 381 | Alaska Division of Geological & Geophysical

Science.gov Websites

DGGS GMC 381 Publication Details Title: 1974 summary report of exploration activities, Orange Hill information. Quadrangle(s): Nabesna Bibliographic Reference Trautwein, C.M., 2010, 1974 summary report of exploration activities, Orange Hill, Alaska: Alaska Division of Geological & Geophysical Surveys Geologic

76 FR 50275 - Guidance for the Assessment of Beyond-Design-Basis Aircraft Impacts

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-12

...: Mekonen M. Bayssie, Regulatory Guide Development Branch, Division of Engineering, Office of Nuclear... e-mail to [email protected] . SUPPLEMENTARY INFORMATION: I. Introduction The Nuclear..., Division of Engineering, Office of Nuclear Regulatory Research. [FR Doc. 2011-20513 Filed 8-11-11; 8:45 am...

75 FR 16525 - Notice of Issuance of Regulatory Guide

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-01

... CONTACT: Mekonen M. Bayssie, Regulatory Guide Development Branch, Division of Engineering, Office of...) 251-7489 or e-mail [email protected] . SUPPLEMENTARY INFORMATION: I. Introduction The U.S..., Division of Engineering, Office of Nuclear Regulatory Research. [FR Doc. 2010-7391 Filed 3-31-10; 8:45 am...

Conceptual Assessment Tool for Advanced Undergraduate Electrodynamics

ERIC Educational Resources Information Center

Baily, Charles; Ryan, Qing X.; Astolfi, Cecilia; Pollock, Steven J.

2017-01-01

As part of ongoing investigations into student learning in advanced undergraduate courses, we have developed a conceptual assessment tool for upper-division electrodynamics (E&M II): the Colorado UppeR-division ElectrodyNamics Test (CURrENT). This is a free response, postinstruction diagnostic with 6 multipart questions, an optional 3-question…

The TORMOZ Gene Encodes a Nucleolar Protein Required for Regulated Division Planes and Embryo Development in Arabidopsis[W

PubMed Central

Griffith, Megan E.; Mayer, Ulrike; Capron, Arnaud; Ngo, Quy A.; Surendrarao, Anandkumar; McClinton, Regina; Jürgens, Gerd; Sundaresan, Venkatesan

2007-01-01

Embryogenesis in Arabidopsis thaliana is marked by a predictable sequence of oriented cell divisions, which precede cell fate determination. We show that mutation of the TORMOZ (TOZ) gene yields embryos with aberrant cell division planes and arrested embryos that appear not to have established normal patterning. The defects in toz mutants differ from previously described mutations that affect embryonic cell division patterns. Longitudinal division planes of the proembryo are frequently replaced by transverse divisions and less frequently by oblique divisions, while divisions of the suspensor cells, which divide only transversely, appear generally unaffected. Expression patterns of selected embryo patterning genes are altered in the mutant embryos, implying that the positional cues required for their proper expression are perturbed by the misoriented divisions. The TOZ gene encodes a nucleolar protein containing WD repeats. Putative TOZ orthologs exist in other eukaryotes including Saccharomyces cerevisiae, where the protein is predicted to function in 18S rRNA biogenesis. We find that disruption of the Sp TOZ gene results in cell division defects in Schizosaccharomyces pombe. Previous studies in yeast and animal cells have identified nucleolar proteins that regulate the exit from M phase and cytokinesis, including factors involved in pre-rRNA processing. Our study suggests that in plant cells, nucleolar functions might interact with the processes of regulated cell divisions and influence the selection of longitudinal division planes during embryogenesis. PMID:17616738

Cortical PAR polarity proteins promote robust cytokinesis during asymmetric cell division

PubMed Central

Jordan, Shawn N.; Davies, Tim; Zhuravlev, Yelena; Dumont, Julien; Shirasu-Hiza, Mimi

2016-01-01

Cytokinesis, the physical division of one cell into two, is thought to be fundamentally similar in most animal cell divisions and driven by the constriction of a contractile ring positioned and controlled solely by the mitotic spindle. During asymmetric cell divisions, the core polarity machinery (partitioning defective [PAR] proteins) controls the unequal inheritance of key cell fate determinants. Here, we show that in asymmetrically dividing Caenorhabditis elegans embryos, the cortical PAR proteins (including the small guanosine triphosphatase CDC-42) have an active role in regulating recruitment of a critical component of the contractile ring, filamentous actin (F-actin). We found that the cortical PAR proteins are required for the retention of anillin and septin in the anterior pole, which are cytokinesis proteins that our genetic data suggest act as inhibitors of F-actin at the contractile ring. Collectively, our results suggest that the cortical PAR proteins coordinate the establishment of cell polarity with the physical process of cytokinesis during asymmetric cell division to ensure the fidelity of daughter cell formation. PMID:26728855

Efficient Production of 4-KeV X Rays from Laser-Heated Xe Gas = Confined Within a Hohlraum

NASA Astrophysics Data System (ADS)

Grun, Jacob; Suter, Larry J.; Back, Christina A.; Decker, Chris; Kauffman, Robert L.; Davis, John F.

1996-11-01

Clean (debris-free) and efficient multi-kilovolt x-ray sources are needed for irradiating large military test objects and for use as backlighters in future Inertial Confinement Fusion experiments. Laser-plasma x-ray sources are particularly attractive for these uses since their spectrum can be controlled by proper choice of plasma material and laser intensity; and because many laser-plasma sources can be designed to produce little or no particulate debris. We report on an experiment in which we measured the production-efficiency, spectrum, and time history of 1-4 KeV x-rays from beryllium hohlraums which were filled with 1 and 2 atm of Xe gas and then irradiated by a 2-nsec pulse from the NOVA laser. It is predicted that 17be converted into > 4KeV x rays and 30history of >4KeV part of the spectrum is predicted to exhibit a dip in intensity whose depth and location vary with fill pressure and hohlraum size.. We also measured the debris produced by these sources. Work supported by the Defense Special Weapons Agency and the U.S. Department of Energy at LLNL under W-7405-ENG-48.

An adaptive optics search for young extrasolar planets

NASA Astrophysics Data System (ADS)

Macintosh, B.; Zuckerman, B.; Becklin, E. E.; Kaisler, D.; Lowrance, P.; Max, C. E.; Olivier, S.

2000-10-01

In the past five years, many extrasolar planets have been detected indirectly, through radial velocity variations induced in their parent stars. Advances in technology now open up the possibility of directly detecting extrasolar planets through the photons they emit. Direct detection would allow determination of the temperature, radius, and composition of a planet, particularly one in a wide orbit - an important complement to radial velocity techniques. Seeing a planet against the halo of scattered light from its parent star is extremely challenging, but adaptive optics (AO) on 8-10 m telescopes can make this possible. The first such large-telescope AO system is now operational on the 10-m W.M. Keck II telescope. Its current performance is sufficient to detect objects at contrast ratios of 105 at separations of 1" and 106 at 2". This is insufficient to detect the reflected light from a mature Jupiter-like planet, but we can easily detect the near-infrared thermal emission from young (<10-50 MYr) planets, or older brown dwarfs. We are carrying out a search for such planetary companions to young nearby stars, including the TW Hydrae association. We present preliminary results from this survey, including sensitivity limits and follow-up of candidate companions originally detected by NICMOS. We have also imaged the Epsilon Eridani system, and present upper limits on the brightness of the planet detected via radial velocity variations by Cochran et al. This research was performed under the auspices of the U.S. Department of Energy by Lawrence Livermore National Laboratory under Contract W-7405-ENG-48, and also supported in part by the Center for Adaptive Optics under the STC Program of the National Science Foundation under Agreement No. AST-9876783

Control of cell division in Streptococcus pneumoniae by the conserved Ser/Thr protein kinase StkP.

PubMed

Beilharz, Katrin; Nováková, Linda; Fadda, Daniela; Branny, Pavel; Massidda, Orietta; Veening, Jan-Willem

2012-04-10

How the human pathogen Streptococcus pneumoniae coordinates cell-wall synthesis during growth and division to achieve its characteristic oval shape is poorly understood. The conserved eukaryotic-type Ser/Thr kinase of S. pneumoniae, StkP, previously was reported to phosphorylate the cell-division protein DivIVA. Consistent with a role in cell division, GFP-StkP and its cognate phosphatase, GFP-PhpP, both localize to the division site. StkP localization depends on its penicillin-binding protein and Ser/Thr-associated domains that likely sense uncross-linked peptidoglycan, because StkP and PhpP delocalize in the presence of antibiotics that target the latest stages of cell-wall biosynthesis and in cells that have stopped dividing. Time-lapse microscopy shows that StkP displays an intermediate timing of recruitment to midcell: StkP arrives shortly after FtsA but before DivIVA. Furthermore, StkP remains at midcell longer than FtsA, until division is complete. Cells mutated for stkP are perturbed in cell-wall synthesis and display elongated morphologies with multiple, often unconstricted, FtsA and DivIVA rings. The data show that StkP plays an important role in regulating cell-wall synthesis and controls correct septum progression and closure. Overall, our results indicate that StkP signals information about the cell-wall status to key cell-division proteins and in this way acts as a regulator of cell division.

A Thermal Management System Using Ammonium Carbamate as an Endothermic Heat Sink (POSTPRINT)

DTIC Science & Technology

2017-04-01

Niedbalski and Soumya S. Patnaik Mechanical and Thermal Systems Branch Power and Control Division Douglas J. Johnson and Jamie S. Ervin University...failing to comply with a collection of information if it does not display a currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE...and Control Division Air Force Research Laboratory, Aerospace Systems Directorate Wright-Patterson Air Force Base, OH 45433-7542 Air Force Materiel

Functional group interactions with single wall carbon NT studied by ab-initio calculations

NASA Astrophysics Data System (ADS)

Cicero, Giancarlo

2005-03-01

With the goal of designing functionalized nanotube materials, recent AFM measurements have succeeded in determining the force between individual chemical groups an single-wall carbon nanotubes (SWCNT) [1]. In order to rationalize and understand these experimental results, we have performed Density Functional Theory calculations for a number of structural arrangements of model tips functionalized with the same groups as those used experimentally. Our calculations include full geometry optimization of the composite SWCNT/tip system as well as `pulling-out' simulations to compute interaction forces. We considered (14x0), semi- conducting tubes, and AFM tips where modeled by a SiH3CH2-X molecule, with X- representing -CN, -CH3, -NH2 or -CH2OCH2. As X is varied, computed forces reproduce the same trend as that observed experimentally when n-doped SWCNT are considered; significantly different trends are observed for neutral and p-doped tubes. We propose that the polar solvent present in the experimental setup may be responsible for the n-doping of the nanotube suggested by our calculations. This work was performed under the auspices of the U.S. Department of Energy by University of California Lawrence Livermore National Laboratory under contract No. W-7405-Eng-48. [1] M.C. LeMieux et al, preprint

Drosophila tubulin-binding cofactor B is required for microtubule network formation and for cell polarity

PubMed Central

Baffet, Alexandre D.; Benoit, Béatrice; Januschke, Jens; Audo, Jennifer; Gourhand, Vanessa; Roth, Siegfried; Guichet, Antoine

2012-01-01

Microtubules (MTs) are essential for cell division, shape, intracellular transport, and polarity. MT stability is regulated by many factors, including MT-associated proteins and proteins controlling the amount of free tubulin heterodimers available for polymerization. Tubulin-binding cofactors are potential key regulators of free tubulin concentration, since they are required for α-β–tubulin dimerization in vitro. In this paper, we show that mutation of the Drosophila tubulin-binding cofactor B (dTBCB) affects the levels of both α- and β-tubulins and dramatically destabilizes the MT network in different fly tissues. However, we find that dTBCB is dispensable for the early MT-dependent steps of oogenesis, including cell division, and that dTBCB is not required for mitosis in several tissues. In striking contrast, the absence of dTBCB during later stages of oogenesis causes major defects in cell polarity. We show that dTBCB is required for the polarized localization of the axis-determining mRNAs within the oocyte and for the apico-basal polarity of the surrounding follicle cells. These results establish a developmental function for the dTBCB gene that is essential for viability and MT-dependent cell polarity, but not cell division. PMID:22855530

78 FR 65718 - Notice of Applications for Deregistration Under Section 8(f) of the Investment Company Act of 1940

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-01

... (202) 551-6810, SEC, Division of Investment Management, Exemptive Applications Office, 100 F Street NE... incurred in connection with the liquidation were paid by SEI Investments Management Corporation, applicant... Division of Investment Management, pursuant to delegated authority. Kevin M. O'Neill, Deputy Secretary. [FR...

Geometric Reasoning in an Active-Engagement Upper-Division E&M Classroom

ERIC Educational Resources Information Center

Cerny, Leonard Thomas

2012-01-01

A combination of theoretical perspectives is used to create a rich description of student reasoning when facing a highly-geometric electricity and magnetism problem in an upper-division active-engagement physics classroom at Oregon State University. Geometric reasoning as students encounter problem situations ranging from familiar to novel is…

Publications - RI 94-28 | Alaska Division of Geological & Geophysical

Science.gov Websites

Fault, southcentral Alaska Authors: Combellick, R.A., Cruse, G.R., and Hammond, W.R. Publication Date profiles across the Castle Mountain Fault, southcentral Alaska: Alaska Division of Geological & Fault, southcentral Alaska, scale 1:40,000 (715.0 M) Keywords Castle Mountain Fault; Faults; Geophysical

PARTICLE ACCELERATOR DIVISION SUMMARY REPORT FOR NOVEMBER 1958 THROUGH MAY 1959

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

Work in the division is summarized in the areas of theoretical studies, model magnet studies, ring magnet vacuum chamber, vacuum pumping system, ring magnet power supply, radio-frequency system, injection system, theoretical studies on radial motion through the linac, outgassing, and ferrite bonding. (For preceding period see ANL-5956.) (W.D.M.)

Inhibition of phenylpropanoid biosynthesis increases cell wall digestibility, protoplast isolation, and facilitates sustained cell division in American elm (Ulmus americana).

PubMed

Jones, A Maxwell P; Chattopadhyay, Abhishek; Shukla, Mukund; Zoń, Jerzy; Saxena, Praveen K

2012-05-30

Protoplast technologies offer unique opportunities for fundamental research and to develop novel germplasm through somatic hybridization, organelle transfer, protoclonal variation, and direct insertion of DNA. Applying protoplast technologies to develop Dutch elm disease resistant American elms (Ulmus americana L.) was proposed over 30 years ago, but has not been achieved. A primary factor restricting protoplast technology to American elm is the resistance of the cell walls to enzymatic degradation and a long lag phase prior to cell wall re-synthesis and cell division. This study suggests that resistance to enzymatic degradation in American elm was due to water soluble phenylpropanoids. Incubating tobacco (Nicotiana tabacum L.) leaf tissue, an easily digestible species, in aqueous elm extract inhibits cell wall digestion in a dose dependent manner. This can be mimicked by p-coumaric or ferulic acid, phenylpropanoids known to re-enforce cell walls. Culturing American elm tissue in the presence of 2-aminoindane-2-phosphonic acid (AIP; 10-150 μM), an inhibitor of phenylalanine ammonia lyase (PAL), reduced flavonoid content, decreased tissue browning, and increased isolation rates significantly from 11.8% (±3.27) in controls to 65.3% (±4.60). Protoplasts isolated from callus grown in 100 μM AIP developed cell walls by day 2, had a division rate of 28.5% (±3.59) by day 6, and proliferated into callus by day 14. Heterokaryons were successfully produced using electrofusion and fused protoplasts remained viable when embedded in agarose. This study describes a novel approach of modifying phenylpropanoid biosynthesis to facilitate efficient protoplast isolation which has historically been problematic for American elm. This isolation system has facilitated recovery of viable protoplasts capable of rapid cell wall re-synthesis and sustained cell division to form callus. Further, isolated protoplasts survived electrofusion and viable heterokaryons were produced. Together, these results provide the first evidence of sustained cell division, callus regeneration, and potential application of somatic cell fusion in American elm, suggesting that this source of protoplasts may be ideal for genetic manipulation of this species. The technological advance made with American elm in this study has potential implications in other woody species for fundamental and applied research which require availability of viable protoplasts.

Inhibition of phenylpropanoid biosynthesis increases cell wall digestibility, protoplast isolation, and facilitates sustained cell division in American elm (Ulmus americana)

PubMed Central

2012-01-01

Background Protoplast technologies offer unique opportunities for fundamental research and to develop novel germplasm through somatic hybridization, organelle transfer, protoclonal variation, and direct insertion of DNA. Applying protoplast technologies to develop Dutch elm disease resistant American elms (Ulmus americana L.) was proposed over 30 years ago, but has not been achieved. A primary factor restricting protoplast technology to American elm is the resistance of the cell walls to enzymatic degradation and a long lag phase prior to cell wall re-synthesis and cell division. Results This study suggests that resistance to enzymatic degradation in American elm was due to water soluble phenylpropanoids. Incubating tobacco (Nicotiana tabacum L.) leaf tissue, an easily digestible species, in aqueous elm extract inhibits cell wall digestion in a dose dependent manner. This can be mimicked by p-coumaric or ferulic acid, phenylpropanoids known to re-enforce cell walls. Culturing American elm tissue in the presence of 2-aminoindane-2-phosphonic acid (AIP; 10-150 μM), an inhibitor of phenylalanine ammonia lyase (PAL), reduced flavonoid content, decreased tissue browning, and increased isolation rates significantly from 11.8% (±3.27) in controls to 65.3% (±4.60). Protoplasts isolated from callus grown in 100 μM AIP developed cell walls by day 2, had a division rate of 28.5% (±3.59) by day 6, and proliferated into callus by day 14. Heterokaryons were successfully produced using electrofusion and fused protoplasts remained viable when embedded in agarose. Conclusions This study describes a novel approach of modifying phenylpropanoid biosynthesis to facilitate efficient protoplast isolation which has historically been problematic for American elm. This isolation system has facilitated recovery of viable protoplasts capable of rapid cell wall re-synthesis and sustained cell division to form callus. Further, isolated protoplasts survived electrofusion and viable heterokaryons were produced. Together, these results provide the first evidence of sustained cell division, callus regeneration, and potential application of somatic cell fusion in American elm, suggesting that this source of protoplasts may be ideal for genetic manipulation of this species. The technological advance made with American elm in this study has potential implications in other woody species for fundamental and applied research which require availability of viable protoplasts. PMID:22646730

International Federation of Library Associations Annual Conference Papers. Bibliographic Control Division: Bibliography and Cataloguing Sections (47th, Leipzig, East Germany, August 17-22, 1981).

ERIC Educational Resources Information Center

Vita, Susan H.; And Others

This set of papers delivered to the Bibliographic Control Division of the International Federation of Library Associations (IFLA) during its 47th annual conference (1981) includes: "Cataloging in Publication in the United States--Problems and Prospects," by Susan H. Vita; "Development and Coordination of Bibliographic Activities:…

Chromosome segregation drives division site selection in Streptococcus pneumoniae.

PubMed

van Raaphorst, Renske; Kjos, Morten; Veening, Jan-Willem

2017-07-18

Accurate spatial and temporal positioning of the tubulin-like protein FtsZ is key for proper bacterial cell division. Streptococcus pneumoniae (pneumococcus) is an oval-shaped, symmetrically dividing opportunistic human pathogen lacking the canonical systems for division site control (nucleoid occlusion and the Min-system). Recently, the early division protein MapZ was identified and implicated in pneumococcal division site selection. We show that MapZ is important for proper division plane selection; thus, the question remains as to what drives pneumococcal division site selection. By mapping the cell cycle in detail, we show that directly after replication both chromosomal origin regions localize to the future cell division sites, before FtsZ. Interestingly, Z-ring formation occurs coincidently with initiation of DNA replication. Perturbing the longitudinal chromosomal organization by mutating the condensin SMC, by CRISPR/Cas9-mediated chromosome cutting, or by poisoning DNA decatenation resulted in mistiming of MapZ and FtsZ positioning and subsequent cell elongation. Together, we demonstrate an intimate relationship between DNA replication, chromosome segregation, and division site selection in the pneumococcus, providing a simple way to ensure equally sized daughter cells.

User Guide for Unmanned Aerial System (UAS) Operations on the National Ranges

DTIC Science & Technology

2007-11-01

WARFARE CENTER WEAPONS DIVISION, PT. MUGU NAVAL AIR WARFARE CENTER WEAPONS DIVISION, CHINA LAKE NAVAL AIR WARFARE CENTER AIRCRAFT DIVISION, PATUXENT...with IFR Instrument Flight Rules MRTFB Major Range and Test Facility Base NAS National Airspace System NM nautical mile NTIA National...sectional charts, Instrument Flight Rules ( IFR ) enroute charts, and terminal area charts. The floor and ceiling, operating hours, and controlling

Aligned Carbon Nanotube Carpets on Carbon Substrates for High Power Electronic Applications

DTIC Science & Technology

2016-06-01

SiOx by a vapor-solid-solid mechanism ,” J. Am. Chem. Soc., vol. 133, pp. 197–199, 2011. [146] B. Liu, W. Ren, C. Liu, C.-H. Sun , L. Gao, S. Li, C... Mechanical and Thermal Systems Branch Power and Control Division JUNE 2016 Interim Report DISTRIBUTION STATEMENT A: Approved for public release...Advisor Program Engineer Mechanical and Thermal Systems Branch Mechanical and Thermal Systems Branch Power and Control Division Power and Control

AAPG forms environmental unit; Geosat moving

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1992-07-20

This paper reports that the American Association of Petroleum Geologists has established a new environmental geology division, while a geology related committee is moving from Oklahoma. AAPG set up a Division of Environmental Geosciences to provide services and information to its members working in environmental geosciences, the Bernold M. Bruno Hanson of Midland, Tex., the DEG's first president. The division will publish a newsletter in October, and plans are being set to solicit technical articles for consideration by the AAPG Bulletin and for a possible future separate DEG technical publication.

Cytoplasmic removal, enucleation, and cell fusion of mouse oocytes.

PubMed

Kyogoku, Hirohisa; Yoshida, Shuhei; Kitajima, Tomoya S

2018-01-01

Meiotic divisions in females occur in fully grown oocytes that have a large cytoplasmic volume. The intracellular processes that are needed to accomplish meiotic divisions, such as spindle formation, chromosome segregation, and polar body extrusion, are controlled by the concerted actions of nuclear and cytoplasmic factors, which exhibit dynamic quantitative and spatiotemporal changes during meiotic maturation. Thus, distinguishing between meiotic controls that are mediated by cytoplasmic factors and those mediated by nuclear factors helps in the understanding of the mechanisms underlying meiotic divisions. Here, we describe a method to artificially modify the number of nuclei and the volume of the cytoplasm of mouse oocytes through cytoplasmic removal, enucleation, and cell fusion. The oocytes generated by this method are viable and undergo reproducible meiotic divisions exhibiting the effects of altered amounts of cytoplasmic and nuclear factors, which can be analyzed by various assays, such as live imaging microscopy. © 2018 Elsevier Inc. All rights reserved.

Body Size Changes Among National Collegiate Athletic Association New England Division III Football Players, 1956−2014: Comparison With Age-Matched Population Controls

PubMed Central

Elliott, Kayla R.; Harmatz, Jerold S.; Zhao, Yanli; Greenblatt, David J.

2016-01-01

Context:  Collegiate football programs encourage athletes to pursue high body weights. Objective:  To examine position-dependent trends over time in body size characteristics among football players in the National Collegiate Athletic Association Division III New England Small College Athletic Conference (NESCAC) from 1956 to 2014 and to compare the observed absolute and relative changes with those in age-matched male population controls. Design:  Descriptive laboratory study. Setting:  Medical school affiliated with a NESCAC institution. Patients or Other Participants:  Football team rosters from the 10-member NESCAC schools, available as public documents, were analyzed along with body size data from general population males aged 20 to 29 years from the National Health and Nutrition Examination Survey (NHANES). Main Outcome Measure(s):  Body weight, height, and calculated body mass index were evaluated using analysis of variance, linear regression, and nonlinear regression to determine the distribution features of size variables and changes associated with time (year), school, and position. Results:  Among NESCAC linemen, absolute and relative changes over time in body weight and body mass index exceeded corresponding changes in the NHANES population controls. New England Small College Athletic Conference offensive linemen body weights increased by 37.5% from 1956 to 2014 (192 to 264 lb [86.4 to 118.8 kg]), compared with a 12% increase (164 to 184 lb [73.8 to 82.8 kg]) since 1961 in the NHANES population controls. Body mass index changed in parallel with body weight and exceeded 35 kg/m2 in more than 30% of contemporary NESCAC offensive linemen. Among skill players in the NESCAC group, time-related changes in body size characteristics generally paralleled those in the NHANES controls. Conclusions:  High body weight and body mass indices were evident in offensive linemen, even among those in Division III football programs with no athletic scholarships. These characteristics may be associated with adverse cardiovascular and metabolic outcomes. We need approaches to encourage risk modification in the postfootball lifestyles of these individuals. PMID:27159189

Timing of distant flap pedicle division using xenon 133 clearance

DOE Office of Scientific and Technical Information (OSTI.GOV)

Snelling, C.F.; Poomee, A.; Sutherland, J.B.

1980-09-01

Clearance of intradermally injected xenon 133 was used to measure blood flow in distant flaps in humans with the donor pedicle temporarily clamped just prior to division. All 18 flaps with a blood flow of 0.5 ml per 100 gm of tissue per minute or more survived completely after separation. Of 7 with lesser flow, 3 underwent marginal necrosis adjacent to the line of division and 4 survived entirely. Xenon 133 washout does permit quantitative evaluation of blood flow, and since it is a clean isotope, it appears superior to sodium 24 and technetium 99m, which have been used inmore » a similar manner. The test is proposed as an adjunct to clinical judgment in timing pedicle division.« less

MICROTIA AND HEARING DEFICITS IN CD-1 MICE FOLLOWING GESTATIONAL EXPOSURE TO ETHANE DIMETHANESULFONATE (EDS)

EPA Science Inventory

TARKA1,2, D.K., G.R. KLINEFELTER1,2, D.W. HERR3, and J.M. ROGERS 1,2. 1University of North Carolina, School of Medicine, Curriculum in Toxicology, Chapel Hill, North Carolina; and USEPA, NHEERL, 2Reproductive Toxicology Division and 3Neurotoxicology Division, Research Triangle Pa...

Fuels management in the southern Appalachian Mountains, hot continental division

Treesearch

Matthew J. Reilly; Thomas A. Waldrop; Joseph J. O’Brien

2012-01-01

The Southern Appalachian Mountains, Hot Continental Mountains Division, M220 (McNab and others 2007) are a topographically and biologically complex area with over 10 million ha of forested land, where complex environmental gradients have resulted in a great diversity of forest types. Abundant moisture and a long, warm growing season support high levels of productivity...

Publications - PIR 2001-3A | Alaska Division of Geological & Geophysical

Science.gov Websites

content DGGS PIR 2001-3A Publication Details Title: Geologic map of the Eagle A-2 Quadrangle, Fortymile ): Eagle Bibliographic Reference Werdon, M.B., Newberry, R.J., Szumigala, D.J., and Pinney, D.S., 2001 , Geologic map of the Eagle A-2 Quadrangle, Fortymile mining district, Alaska: Alaska Division of Geological

77 FR 73064 - Notice of Applications for Deregistration Under Section 8(f) of the Investment Company Act of 1940

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-07

...) 551-6810, SEC, Division of Investment Management, Office of Investment Company Regulation, 100 F... the Division of Investment Management, pursuant to delegated authority. Kevin M. O'Neill, Deputy... Deregistration Under Section 8(f) of the Investment Company Act of 1940 November 30, 2012. The following is a...

Observations on Student Difficulties with Mathematics in Upper-Division Electricity and Magnetism

ERIC Educational Resources Information Center

Pepper, Rachel E.; Chasteen, Stephanie V.; Pollock, Steven J.; Perkins, Katherine K.

2012-01-01

We discuss common difficulties in upper-division electricity and magnetism (E&M) in the areas of Gauss's law, vector calculus, and electric potential using both quantitative and qualitative evidence. We also show that many of these topical difficulties may be tied to student difficulties with mathematics. At the junior level, some students…

Publications - RI 2013-2 | Alaska Division of Geological & Geophysical

Science.gov Websites

content DGGS RI 2013-2 Publication Details Title: Surficial-geologic map of the Livengood area, central Burns, P.A.C., 2013, Surficial-geologic map of the Livengood area, central Alaska: Alaska Division of Sheet 1 Surficial-geologic map of the Livengood area, central Alaska, scale 1:50,000 (30.0 M) Digital

Opportunistic Access in Frequency Hopping Cognitive Radio Networks

DTIC Science & Technology

2014-03-27

thresholding MA multiple access MFSK M-ary frequency shift keying MIMO multiple-input/multiple-output OFDM orthogonal frequency-division multiplexing x...adaptive BER performance as a function of ISR with orthogonal frequency-division multiplexing ( OFDM ) interference present. . . . . . . . . . 41 4.15 Non...adaptive BER performance as a function of EB/N0 with OFDM interfer- ence present

Advanced Aircraft Electrical System Control Technology Demonstrator. Phase I. Requirements Analysis and Conceptual Design.

DTIC Science & Technology

1981-07-01

System 13 (7) Flight Critical Power 15 (8) Power Bus Configuration 16 b. System Control and Protection 20...includes the main buses, external power receptacles and distribution feeders. The function of the distribution protection system * is mainly to provide...TechnicaI rea Manager Power Systems Branch Power Systems B nch Aerospace Power Division Aerospace Power Division FOR .AKE D . REAMS Chief,

IFLA General Conference, 1990. Division of Bibliographic Control: Open Forum; Section of Cataloguing; Section of Bibliography; Section of Classification and Indexing. Booklet 4.

ERIC Educational Resources Information Center

International Federation of Library Associations, The Hague (Netherlands).

The 10 papers in this collection were presented at an open forum and meetings of three sections within the Division of Bibliographic Control: (1) "Review of the Work of the Section on Cataloguing 1989/1990" (Nancy R. John and Inger Cathrine Spangen); (2) "Report from the Section on Classification and Indexing" (Dorothy…

Annual Report, Reservoir Control Center, Southwestern Division (1989)

DTIC Science & Technology

1990-01-01

Division in the water quality field . This provides for water quality objectives being included as an effective part of our total water management...WES) selected Canyon Lake as a research field site for developing techniques to evaluate the impacts associated with installation of hydropower at Corps...term continuous goals of this Division, and consequently the Water Management Branch, in the water quality field . (1) To obtain sufficient water

Distributed Processing Tools Definition. Volume 1. Hardware and Software Technologies for Tightly-Coupled Distributed Systems.

DTIC Science & Technology

1983-06-01

LOSARDO Project Engineer APPROVED: .MARMCINIhI, Colonel. USAF Chief, Coaud and Control Division FOR THE CCOaIDKR: Acting Chief, Plea Off ice * **711...WORK UNIT NUMBERS General Dynamics Corporation 62702F Data Systems Division P 0 Box 748, Fort Worth TX 76101 55811829 I1. CONTROLLING OFFICE NAME AND...Processing System for 29 the Operation/Direction Center(s) 4-3 Distribution of Processing Control 30 for the Operation/Direction Center(s) 4-4 Generalized

Kinetic simulation of hydrodynamic equivalent capsule implosions

NASA Astrophysics Data System (ADS)

Kwan, Thomas; Le, Ari; Schmitt, Mark; Herrmann, Hans

2016-10-01

We have carried out simulations of direct-drive hydrodynamic equivalent capsule implosion experiments conducted on Omega laser facility at the Laboratory of Laser Energetics of the University of Rochester. The capsules had a glass shell (SiO2) 4.87 μm with an inner diameter of 1086 μm. One was filled with deuterium (D) and tritium (T) at 6.635 and 2.475 atmospheric pressure respectively. The other capsule with D, T, and He-3 at 2.475, 2.475, and 5.55 atmospheric pressure respectively. The capsules were imploded with 60 laser beams with a square pulse length of 0.6ns of total energy of 15.6 kJ. One-dimensional radiation hydrodynamic calculations with HYDRA and kinetic particle/hybrid simulations with LSP are carried out for the post-shot analysis. HYDRA outputs at 0.6ns are linked to LSP, in which the electrons are treated as a fluid while all the ion dynamics is simulated by the standard particle-in-cell technique. Additionally, simulations with the new photon package in LSP are initiated at the beginning of the implosion to include the implosion phase of the capsule. The simulation results of density, temperature, and velocity profiles of the electrons, D, T, He-3, and SiO2species are compared with HYDRA. Detail comparisons among the kinetic simulations, rad-hydro simulations, and experimental results of neutron yield, yield ratio, fusion burn histories, and shell convergence will be presented to assess plasma kinetic effects. Work performed under the auspices of the US DOE by the Los Alamos National Laboratory under Contract No. W7405-ENG-36.

BASL and EPF2 act independently to regulate asymmetric divisions during stomatal development

PubMed Central

Hunt, Lee

2010-01-01

The initiation of stomatal development in the developing Arabidopsis epidermis is characterized by an asymmetric ‘entry’ division in which a small cell, known as a meristemoid, and a larger daughter cell is formed. The meristemoid may undergo further asymmetric divisions, regenerating a meristemoid each time, before differentiating into a guard mother cell which divides symmetrically to form a pair of guard cells surrounding a stomatal pore. Recently EPF2 and BASL have emerged as regulators of these asymmetric divisions and here we present results indicating that these two factors operate independently to control stomatal development PMID:20220310

An All-Optical Access Metro Interface for Hybrid WDM/TDM PON Based on OBS

NASA Astrophysics Data System (ADS)

Segarra, Josep; Sales, Vicent; Prat, Josep

2007-04-01

A new all-optical access metro network interface based on optical burst switching (OBS) is proposed. A hybrid wavelength-division multiplexing/time-division multiplexing (WDM/TDM) access architecture with reflective optical network units (ONUs), an arrayed-waveguide-grating outside plant, and a tunable laser stack at the optical line terminal (OLT) is presented as a solution for the passive optical network. By means of OBS and a dynamic bandwidth allocation (DBA) protocol, which polls the ONUs, the available access bandwidth is managed. All the network intelligence and costly equipment is located at the OLT, where the DBA module is centrally implemented, providing quality of service (QoS). To scale this access network, an optical cross connect (OXC) is then used to attain a large number of ONUs by the same OLT. The hybrid WDM/TDM structure is also extended toward the metropolitan area network (MAN) by introducing the concept of OBS multiplexer (OBS-M). The network element OBS-M bridges the MAN and access networks by offering all-optical cross connection, wavelength conversion, and data signaling. The proposed innovative OBS-M node yields a full optical data network, interfacing access and metro with a geographically distributed access control. The resulting novel access metro architectures are nonblocking and, with an improved signaling, provide QoS, scalability, and very low latency. Finally, numerical analysis and simulations demonstrate the traffic performance of the proposed access scheme and all-optical access metro interface and architectures.

CedA is a novel Escherichia coli protein that activates the cell division inhibited by chromosomal DNA over-replication.

PubMed

Katayama, T; Takata, M; Sekimizu, K

1997-11-01

We isolated and characterized a new gene related to the control of cell division regulation in Escherichia coli. At 30 degrees C, the dnaAcos mutant causes over-replication of the chromosome, and colony formation is inhibited. We found that, at this temperature, the dnaAcos cells form filaments; therefore, septum formation is inhibited. This inhibition was independent of SfiA, an inhibitor of the septum-forming protein, FtsZ. To identify factors involved in this pathway of inhibition, we isolated seven multicopy suppressors for the cold-sensitive phenotype of the dnaAcos mutant. One of these proved to be a previously unknown gene, which we named cedA. This gene encoded a 12 kDa protein and resided at 38.9min on the E. coli genome map. A multicopy supply of the cedA gene to the dnaAcos cells did not repress over-replication of the chromosome but did stimulate cell division of the host, the result being growth of cells with an abnormally elevated chromosomal copy number. Therefore, the expression level of the cedA gene seems to be important for inhibiting cell division of the dnaAcos mutant at 30 degrees C. We propose that over-replication of the chromosome activates a pathway for inhibiting cell division and that the cedA gene modulates this division control. In the dnaA+ background, cedA also seems to affect cell division.

Wavelength Measurements of Ni L-shell Lines between 9 and 15 A

NASA Astrophysics Data System (ADS)

Gu, Ming F.; Beiersdorfer, P.; Brown, G. V.; Chen, H.; Thorn, D. B.; Kahn, S. M.

2006-09-01

We present accurate wavelength measurements of nikel L-shell X-ray lines resulting from Δ n ≥ 1 transitions (mostly, 2 - 3 transitions) between 9 and 15 Å. We have used the electron beam ion trap, SuperEBIT, at the Lawrence Livermore National Laboratory and a flat field grating spectrometer to record the spectra. Most significant emission lines of Ni XIX -- XXVI in our spectral coverage are identified. The resulting data set provides valuable input in the analyses of high resolution X-ray spectra of stellar coronae sources, including the Sun. This work was performed under the auspices of U.S. DOE contract No. W-7405-Eng-48, and supported by NASA APRA Grant NAG5-5419.

Mechanical Forces Program the Orientation of Cell Division during Airway Tube Morphogenesis.

PubMed

Tang, Zan; Hu, Yucheng; Wang, Zheng; Jiang, Kewu; Zhan, Cheng; Marshall, Wallace F; Tang, Nan

2018-02-05

Oriented cell division plays a key role in controlling organogenesis. The mechanisms for regulating division orientation at the whole-organ level are only starting to become understood. By combining 3D time-lapse imaging, mouse genetics, and mathematical modeling, we find that global orientation of cell division is the result of a combination of two types of spindles with distinct spindle dynamic behaviors in the developing airway epithelium. Fixed spindles follow the classic long-axis rule and establish their division orientation before metaphase. In contrast, rotating spindles do not strictly follow the long-axis rule and determine their division orientation during metaphase. By using both a cell-based mechanical model and stretching-lung-explant experiments, we showed that mechanical force can function as a regulatory signal in maintaining the stable ratio between fixed spindles and rotating spindles. Our findings demonstrate that mechanical forces, cell geometry, and oriented cell division function together in a highly coordinated manner to ensure normal airway tube morphogenesis. Copyright © 2017 Elsevier Inc. All rights reserved.

Journey of oocyte from metaphase-I to metaphase-II stage in mammals.

PubMed

Sharma, Alka; Tiwari, Meenakshi; Gupta, Anumegha; Pandey, Ashutosh N; Yadav, Pramod K; Chaube, Shail K

2018-08-01

In mammals, journey from metaphase-I (M-I) to metaphase-II (M-II) is important since oocyte extrude first polar body (PB-I) and gets converted into haploid gamete. The molecular and cellular changes associated with meiotic cell cycle progression from M-I to M-II stage and extrusion of PB-I remain ill understood. Several factors drive oocyte meiosis from M-I to M-II stage. The mitogen-activated protein kinase3/1 (MAPK3/1), signal molecules and Rho family GTPases act through various pathways to drive cell cycle progression from M-I to M-II stage. The down regulation of MOS/MEK/MAPK3/1 pathway results in the activation of anaphase-promoting complex/cyclosome (APC/C). The active APC/C destabilizes maturation promoting factor (MPF) and induces meiotic resumption. Several signal molecules such as, c-Jun N-terminal kinase (JNK2), SENP3, mitotic kinesin-like protein 2 (MKlp2), regulator of G-protein signaling (RGS2), Epsin2, polo-like kinase 1 (Plk1) are directly or indirectly involved in chromosomal segregation. Rho family GTPase is another enzyme that along with cell division cycle (Cdc42) to form actomyosin contractile ring required for chromosomal segregation. In the presence of origin recognition complex (ORC4), eccentrically localized haploid set of chromosomes trigger cortex differentiation and determine the division site for polar body formation. The actomyosin contractile activity at the site of division plane helps to form cytokinetic furrow that results in the formation and extrusion of PB-I. Indeed, oocyte journey from M-I to M-II stage is coordinated by several factors and pathways that enable oocyte to extrude PB-I. Quality of oocyte directly impact fertilization rate, early embryonic development, and reproductive outcome in mammals. © 2018 Wiley Periodicals, Inc.

Division by zero, pseudo-division by zero, Zhang dynamics method and Zhang-gradient method about control singularity conquering

NASA Astrophysics Data System (ADS)

Zhang, Yunong; Zhang, Yinyan; Chen, Dechao; Xiao, Zhengli; Yan, Xiaogang

2017-01-01

In this paper, the division-by-zero (DBO) problem in the field of nonlinear control, which is traditionally termed the control singularity problem (or specifically, controller singularity problem), is investigated by the Zhang dynamics (ZD) method and the Zhang-gradient (ZG) method. According to the impact of the DBO problem on the state variables of the controlled nonlinear system, the concepts of the pseudo-DBO problem and the true-DBO problem are proposed in this paper, which provide a new perspective for the researchers on the DBO problems as well as nonlinear control systems. Besides, the two classes of DBO problems are solved under the framework of the ZG method. Specific examples are shown and investigated in this paper to illustrate the two proposed concepts and the efficacy of the ZG method in conquering pseudo-DBO and true-DBO problems. The application of the ZG method to the tracking control of a two-wheeled mobile robot further substantiates the effectiveness of the ZG method. In addition, the ZG method is successfully applied to the tracking control of a pure-feedback nonlinear system.

Fires, A Joint Professional Bulletin for US Field & Air Defense Artillerymen. March-April 2008

DTIC Science & Technology

2008-04-01

actions. However, if the effects synchronization division were not added to the lineup , just the teaming of joint fires division and IO division still...ADAFCO would be the liaison between the two Services who coordinates fires and facilitates track identification thus preventing fratricide. Joint...decisions to be made based on current air users versus planned control measures. Interoperability. Identification is a key feature that enables AC2 nodes

Quantitative Effects of 2,4-Dichlorophenoxyacetic Acid on Growth of Suspension-cultured Acer pseudoplatanus Cells

PubMed Central

Leguay, Jean-Jacques; Guern, Jean

1977-01-01

The utilization of 2,4-dichlorophenoxyacetic acid (2,4-D) molecules by Acer pseudoplatanus cells is governed mainly by a glucosylation process. Evidence that 2,4-D glucoside molecules are biologically inactive is presented. 2,3,5-Triiodobenzoic acid (TIBA), by inhibiting 2,4-D glucosylation, has a sparing effect on 2,4-D molecules; thus TIBA treatments increase growth yield (expressed as the ratio of the maximum number of cells produced to the initial concentration of 2,4-D in the culture medium). Significant amounts of intact 2,4-D molecules remain outside and inside the cells when cell division stops at the onset of the stationary phase. This result and the previous demonstration that, at the onset of the stationary phase, 2,4-D is the specific limiting factor of cell division (Leguay JJ, J Guern 1975 Plant Physiol 56: 356-359) suggest that a threshold concentration of auxin is needed for cell division to proceed. The distribution of 2,4-D molecules between the cells and the culture medium is dependent on the population density at the stationary phase. The extracellular 2,4-D concentration at that time is a linear function of the population density whereas intracellular amounts of 2,4-D and 2,4-D metabolites are constant. By using a modified 2-14C,-5,5-dimethyloxazolidine-2,4-dione technique, it has been shown that the intracellular pH is markedly lowered as the population density at the plateau is increased. This intracellular pH modification is likely to be responsible for a large modification of the ratio between intracellular and extracellular auxin concentrations. The intracellular auxin concentration reaches a constant value (about 3 × 10−7m), independent of population density when cell division stops at the onset of the stationary phase suggesting that it represents the threshold value of the control for cell division. PMID:16660072

Methylglyoxal synthase regulates cell elongation via alterations of cellular methylglyoxal and spermidine content in Bacillus subtilis.

PubMed

Shin, Sang-Min; Song, Sung-Hyun; Lee, Jin-Woo; Kwak, Min-Kyu; Kang, Sa-Ouk

2017-10-01

Methylglyoxal regulates cell division and differentiation through its interaction with polyamines. Loss of their biosynthesizing enzyme causes physiological impairment and cell elongation in eukaryotes. However, the reciprocal effects of methylglyoxal and polyamine production and its regulatory metabolic switches on morphological changes in prokaryotes have not been addressed. Here, Bacillus subtilis methylglyoxal synthase (mgsA) and polyamine biosynthesizing genes encoding arginine decarboxylase (SpeA), agmatinase (SpeB), and spermidine synthase (SpeE), were disrupted or overexpressed. Treatment of 0.2mM methylglyoxal and 1mM spermidine led to the elongation and shortening of B. subtilis wild-type cells to 12.38±3.21μm (P<0.05) and 3.24±0.73μm (P<0.01), respectively, compared to untreated cells (5.72±0.68μm). mgsA-deficient (mgsA - ) and -overexpressing (mgsA OE ) mutants also demonstrated cell shortening and elongation, similar to speB- and speE-deficient (speB - and speE - ) and -overexpressing (speB OE and speE OE ) mutants. Importantly, both mgsA-depleted speB OE and speE OE mutants (speB OE /mgsA - and speE OE /mgsA - ) were drastically shortened to 24.5% and 23.8% of parental speB OE and speE OE mutants, respectively. These phenotypes were associated with reciprocal alterations of mgsA and polyamine transcripts governed by the contents of methylglyoxal and spermidine, which are involved in enzymatic or genetic metabolite-control mechanisms. Additionally, biophysically detected methylglyoxal-spermidine Schiff bases did not affect morphogenesis. Taken together, the findings indicate that methylglyoxal triggers cell elongation. Furthermore, cells with methylglyoxal accumulation commonly exhibit an elongated rod-shaped morphology through upregulation of mgsA, polyamine genes, and the global regulator spx, as well as repression of the cell division and shape regulator, FtsZ. Copyright © 2017 Elsevier Ltd. All rights reserved.

Electromagnetic Energy Deposition in a Concentric Spherical Model of the Human or Animal Head.

DTIC Science & Technology

1979-12-01

by personnel of the Btomthm tics M4odeling Branch, Data Sciences Division, USAF School of Aerospace 4bd - icine, Aerogace Medical Division, AFSC...hood of S, then curl (A) is a vector field such that fcurl ) = A Tds , (22) S C where N and T are, respectively, the unit normals and the unit

Lisa B Signorello, ScD, ScM | Division of Cancer Prevention

Cancer.gov

Lisa Signorello is the Director and Chief of the Cancer Prevention Fellowship Program (CPFP) Branch in the National Cancer Institute's Division of Cancer Prevention. Dr. Signorello served as Deputy Director of the CPFP from August 2014 to November 2017 and came to the NCI after having held academic positions at the Harvard School of Public Health, Harvard Medical School, and

Publications - RI 97-15B | Alaska Division of Geological & Geophysical

Science.gov Websites

content DGGS RI 97-15B Publication Details Title: Interpretive geologic bedrock map of the Tanana B-1 ., 1997, Interpretive geologic bedrock map of the Tanana B-1 Quadrangle, central Alaska: Alaska Division bedrock map of the Tanana B-1 Quadrangle, Central Alaska, scale 1:63,360 (8.3 M) Digital Geospatial Data

Colorado Upper-Division Electrostatics Diagnostic: A Conceptual Assessment for the Junior Level

ERIC Educational Resources Information Center

Chasteen, Stephanie V.; Pepper, Rachel E.; Caballero, Marcos D.; Pollock, Steven J.; Perkins, Katherine K.

2012-01-01

As part of an effort to systematically improve our junior-level E&M I course, we have developed a tool to assess student conceptual learning of electrostatics at the upper division. Together with a group of physics faculty, we established a list of learning goals for the course that, with results from student observations and interviews,…

Interdependency of formation and localisation of the Min complex controls symmetric plastid division.

PubMed

Maple, Jodi; Møller, Simon G

2007-10-01

Plastid division represents a fundamental biological process essential for plant development; however, the molecular basis of symmetric plastid division is unclear. AtMinE1 plays a pivotal role in selection of the plastid division site in concert with AtMinD1. AtMinE1 localises to discrete foci in chloroplasts and interacts with AtMinD1, which shows a similar localisation pattern. Here, we investigate the importance of Min protein complex formation during the chloroplast division process. Dissection of the assembly of the Min protein complex and determination of the interdependency of complex assembly and localisation in planta allow us to present a model of the molecular basis of selection of the division site in plastids. Moreover, functional analysis of AtMinE1 in bacteria demonstrates the level of functional conservation and divergence of the plastidic MinE proteins.

75 FR 20007 - Proposal Review Panel for Chemistry; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-16

... NATIONAL SCIENCE FOUNDATION Proposal Review Panel for Chemistry; Notice of Meeting In accordance... California Institute of Technology by NSF Division of Chemistry (1191). Dates & Times: May 9, 2010; 8 p.m.-9 p.m., May 10, 2010; 8 a.m.- 9 p.m., May 11, 2010; 8 a.m.-1 p.m. Place: Department of Chemistry...

Myo19 ensures symmetric partitioning of mitochondria and coupling of mitochondrial segregation to cell division.

PubMed

Rohn, Jennifer L; Patel, Jigna V; Neumann, Beate; Bulkescher, Jutta; Mchedlishvili, Nunu; McMullan, Rachel C; Quintero, Omar A; Ellenberg, Jan; Baum, Buzz

2014-11-03

During animal cell division, an actin-based ring cleaves the cell into two. Problems with this process can cause chromosome missegregation and defects in cytoplasmic inheritance and the partitioning of organelles, which in turn are associated with human diseases. Although much is known about how chromosome segregation is coupled to cell division, the way organelles coordinate their inheritance during partitioning to daughter cells is less well understood. Here, using a high-content live-imaging small interfering RNA screen, we identify Myosin-XIX (Myo19) as a novel regulator of cell division. Previously, this actin-based motor was shown to control the interphase movement of mitochondria. Our analysis shows that Myo19 is indeed localized to mitochondria and that its silencing leads to defects in the distribution of mitochondria within cells and in mitochondrial partitioning at division. Furthermore, many Myo19 RNAi cells undergo stochastic division failure--a phenotype that can be mimicked using a treatment that blocks mitochondrial fission and rescued by decreasing mitochondrial fusion, implying that mitochondria can physically interfere with cytokinesis. Strikingly, using live imaging we also observe the inappropriate movement of mitochondria to the poles of spindles in cells depleted for Myo19 as they enter anaphase. Since this phenocopies the results of an acute loss of actin filaments in anaphase, these data support a model whereby the Myo19 actin-based motor helps to control mitochondrial movement to ensure their faithful segregation during division. The presence of DNA within mitochondria makes their inheritance an especially important aspect of symmetrical cell division. Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.

New Mission Control Center Briefing

NASA Technical Reports Server (NTRS)

1995-01-01

Live footage shows panelists, Chief Center Systems Division John Muratore, and Acting Chief, Control Center Systems Division, Linda Uljon, giving an overview of the new Mission Control Center. Muratore and Uljon talk about the changes and modernization of the new Center. The panelists mention all the new capabilities of the new Center. They emphasize the Distributed real time command and control environment, the reduction in operation costs, and even the change from coaxial cables to fiber optic cables. Uljon also tells us that the new Control Center will experience its first mission after the launch of STS-70 and its first complete mission (both launching and landing) during STS-71.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

This eighth annual report of the Division covers work done during FY 1981 (October 1, 1980, through September 30, 1981). As with these documents in the past, the format follows approximately the organizational structure of the Energy Division. Chapters 2 through 6 summarize the activities of the sections of the Division: Environmental Impact Section, headed by H.E. Zittel; Regional and Urban Studies Section, R.M. Davis; Economic Analysis Section, R.B. Shelton; Data and Analysis Section, A.S. Loebl; and Efficiency and Renewables Research Section, J.W. Michel. In addition, work on a variety of projects which cut across section lines is reported inmore » Chapter 7, Integrated Programs. These activities are under the supervision of T.J. Wilbanks, Associate Director for the Division. Separate abstracts are included for individual projects.« less

A 16-Channel Distributed-Feedback Laser Array with a Monolithic Integrated Arrayed Waveguide Grating Multiplexer for a Wavelength Division Multiplex-Passive Optical Network System Network

NASA Astrophysics Data System (ADS)

Zhao, Jian-Yi; Chen, Xin; Zhou, Ning; Huang, Xiao-Dong; Cao, Ming-De; Liu, Wen

2014-07-01

A 16-channel distributed-feedback (DFB) laser array with a monolithic integrated arrayed waveguide grating multiplexer for a wavelength division multiplex-passive optical network system is fabricated by using the butt-joint metal organic chemical vapor deposition technology and nanoimpirnt technology. The results show that the threshold current is about 20-30 mA at 25°C. The DFB laser side output power is about 16 mW with a 150 mA injection current. The lasing wavelength is from 1550 nm to 1575 nm covering a more than 25 nm range with 200 GHz channel space. A more than 55 dB sidemode suppression ratio is obtained.

The Nematode Caenorhabditis Elegans.

ERIC Educational Resources Information Center

Kenyon, Cynthia

1988-01-01

Discusses advantages of nematode use for studying patterns of cell division, differentiation, and morphogenesis. Describes nematode development. Cites experimental approaches available for genetic studies. Reviews the topics of control of cell division and differentiation, the nervous system, and muscle assembly and function of the organism. (RT)

Sexual Violence Prevention

MedlinePlus

... Intimate Partner and Sexual Violence Survey National Sexual Violence Resource Center Features Media Sign up for Features Get Email Updates To ... Center for Injury Prevention and Control, Division of Violence Prevention Page maintained by: Office of the Associate Director for Communication, Digital Media Branch, Division of Public Affairs ... ...

SSD1, which encodes a plant-specific novel protein, controls plant elongation by regulating cell division in rice.

PubMed

Asano, Kenji; Miyao, Akio; Hirochika, Hirohiko; Kitano, Hidemi; Matsuoka, Makoto; Ashikari, Motoyuki

2010-01-01

Plant height is one of the most important traits in crop improvement. Therefore revealing the mechanism of plant elongation and controlling plant height in accordance with breeding object is important. In this study we analyzed a novel dwarf mutant, ssd1, of which phenotype is different from typical GA- or BR-related dwarf phenotype. ssd1 exhibits pleiotropic defects in elongation of various organs such as stems, roots, leaves, and flowers. ssd1 also shows abnormal cell files and shapes, which suggests defects of normal cell division in the mutant. Map-based cloning and complementation test demonstrated that the dwarf phenotype in ssd1 mutant was caused by insertion of retrotransposon in a gene, which encodes plant-specific protein with unknown biochemical function. A BLAST search revealed that SSD1-like genes exist in diverse plant species, including monocots and dicots, but not fern and moss. Our results demonstrate that SSD1 controls plant elongation by controlling cell division in higher plants.

Accurate Cell Division in Bacteria: How Does a Bacterium Know Where its Middle Is?

NASA Astrophysics Data System (ADS)

Howard, Martin; Rutenberg, Andrew

2004-03-01

I will discuss the physical principles lying behind the acquisition of accurate positional information in bacteria. A good application of these ideas is to the rod-shaped bacterium E. coli which divides precisely at its cellular midplane. This positioning is controlled by the Min system of proteins. These proteins coherently oscillate from end to end of the bacterium. I will present a reaction-diffusion model that describes the diffusion of the Min proteins, and their binding/unbinding from the cell membrane. The system possesses an instability that spontaneously generates the Min oscillations, which control accurate placement of the midcell division site. I will then discuss the role of fluctuations in protein dynamics, and investigate whether fluctuations set optimal protein concentration levels. Finally I will examine cell division in a different bacteria, B. subtilis. where different physical principles are used to regulate accurate cell division. See: Howard, Rutenberg, de Vet: Dynamic compartmentalization of bacteria: accurate division in E. coli. Phys. Rev. Lett. 87 278102 (2001). Howard, Rutenberg: Pattern formation inside bacteria: fluctuations due to the low copy number of proteins. Phys. Rev. Lett. 90 128102 (2003). Howard: A mechanism for polar protein localization in bacteria. J. Mol. Biol. 335 655-663 (2004).

[Right extremities pain caused by a malacia lesion in the left putamen:a resting functional magnetic resonance imaging of the marginal division of the human brain].

PubMed

Chen, Zhi-Ye; Ma, Lin

2014-04-01

To explore the role of marginal division of the human brain in the pain modulation. Resting functional magnetic resonance imaging was applied in a patient with right extremities pain caused by a malacia lesion in the left putamen and in 8 healthy volunteers. Marginal division was defined using manual drawing on structure images, and was applied to the computation of fuctional connectivity maps. The functional connectivities in the left marginal division showed an evident decrease in the patient when compared with healthy controls. These connectivities were mainly located in the bilateral head of caudate nucleus, putamen, and left globus pallidus. The marginal division may be involved in the pain modulation.

Asymmetric cell division during T cell development controls downstream fate

PubMed Central

Pham, Kim; Shimoni, Raz; Charnley, Mirren; Ludford-Menting, Mandy J.; Hawkins, Edwin D.; Ramsbottom, Kelly; Oliaro, Jane; Izon, David; Ting, Stephen B.; Reynolds, Joseph; Lythe, Grant; Molina-Paris, Carmen; Melichar, Heather; Robey, Ellen; Humbert, Patrick O.; Gu, Min

2015-01-01

During mammalian T cell development, the requirement for expansion of many individual T cell clones, rather than merely expansion of the entire T cell population, suggests a possible role for asymmetric cell division (ACD). We show that ACD of developing T cells controls cell fate through differential inheritance of cell fate determinants Numb and α-Adaptin. ACD occurs specifically during the β-selection stage of T cell development, and subsequent divisions are predominantly symmetric. ACD is controlled by interaction with stromal cells and chemokine receptor signaling and uses a conserved network of polarity regulators. The disruption of polarity by deletion of the polarity regulator, Scribble, or the altered inheritance of fate determinants impacts subsequent fate decisions to influence the numbers of DN4 cells arising after the β-selection checkpoint. These findings indicate that ACD enables the thymic microenvironment to orchestrate fate decisions related to differentiation and self-renewal. PMID:26370500

Real-time time-division color electroholography using a single GPU and a USB module for synchronizing reference light.

PubMed

Araki, Hiromitsu; Takada, Naoki; Niwase, Hiroaki; Ikawa, Shohei; Fujiwara, Masato; Nakayama, Hirotaka; Kakue, Takashi; Shimobaba, Tomoyoshi; Ito, Tomoyoshi

2015-12-01

We propose real-time time-division color electroholography using a single graphics processing unit (GPU) and a simple synchronization system of reference light. To facilitate real-time time-division color electroholography, we developed a light emitting diode (LED) controller with a universal serial bus (USB) module and the drive circuit for reference light. A one-chip RGB LED connected to a personal computer via an LED controller was used as the reference light. A single GPU calculates three computer-generated holograms (CGHs) suitable for red, green, and blue colors in each frame of a three-dimensional (3D) movie. After CGH calculation using a single GPU, the CPU can synchronize the CGH display with the color switching of the one-chip RGB LED via the LED controller. Consequently, we succeeded in real-time time-division color electroholography for a 3D object consisting of around 1000 points per color when an NVIDIA GeForce GTX TITAN was used as the GPU. Furthermore, we implemented the proposed method in various GPUs. The experimental results showed that the proposed method was effective for various GPUs.

Astrophysics and astronomy (Scientific session of the Physical Sciences Division of the Russian Academy of Sciences, 26 January 2011)

NASA Astrophysics Data System (ADS)

2011-10-01

An Astrophysics and Astronomy scientific session of the Physical Sciences Division of the Russian Academy of Sciences (RAS) was held in the Conference Hall of the P N Lebedev Physical Institute, RAS, on 26 January 2011. The following reports were put on the session's agenda posted on the web site www.gpad.ac.ru of the Physical Sciences Division, RAS: (1) Cherepashchuk A M (Sternberg Astronomical Institute, Moscow State University, Moscow) "Investigation of X-ray sources"; (2) Shustov B M (Institute of Astronomy, Russian Academy of Sciences, Moscow) "Asteroid and comet hazards: physical and other aspects"; (3) Sazhin M V (Sternberg Astronomical Institute, Moscow State University, Moscow) "Search for cosmic strings"; (4) Zakharov A F (Russian Federation State Scientific Center 'A I Alikhanov Institute for Theoretical and Experimental Physics', Moscow) "Exoplanet search using gravitational microlensing". Papers written on the basis of the reports are published below. • Optical investigations of X-ray binary systems, A M Cherepashchuk Physics-Uspekhi, 2011, Volume 54, Number 10, Pages 1061-1067 • Asteroid and comet hazards: the role of physical sciences in solving the problem, B M Shustov Physics-Uspekhi, 2011, Volume 54, Number 10, Pages 1068-1071 • Search for cosmic strings using optical and radio astronomy methods, O S Sazhina, M V Sazhin, M Capaccioli, G Longo Physics-Uspekhi, 2011, Volume 54, Number 10, Pages 1072-1077 • Search for exoplanets using gravitational microlensing, A F Zakharov Physics-Uspekhi, 2011, Volume 54, Number 10, Pages 1077-1084

Diel Variations in Optical Properties of Micromonas pusilla, a Prasinophyte

NASA Technical Reports Server (NTRS)

DuRand, Michele D.; Green, Rebecca E.; Sosik, Heidi M.; Olson, Robert J.

2001-01-01

A laboratory experiment was conducted on cultures of Micromonas pusilla, a marine prasinophyte, to investigate how cell growth and division affect the optical properties over the light:dark cycle. Measurements were made of cell size and concentration, attenuation and absorption coefficients, flow cytometric light scattering (in forward and side directions), chlorophyll and carbon content. Refractive index was calculated using the anomalous diffraction approximation Cells were about 1.5 micrometers in diameter and exhibited phased division, with the major division burst occurring during the night. Typical diel variations were observed, with cells increasing in size and light scattering during the day as they photosynthesize and decreasing at night upon division. The cells were in ultradian growth, with more than one division per day, at a light level of 120 Mu-mol photons m/sq/sec. Since these cells are similar in size to small phytoplankton that are typically abundant in field samples, these results can be used in the interpretation of diel variations in light scattering in natural populations of phytoplankton.

Two Forkhead transcription factors regulate the division of cardiac progenitor cells by a Polo-dependent pathway

PubMed Central

Ahmad, Shaad M.; Tansey, Terese R.; Busser, Brian W.; Nolte, Michael T.; Jeffries, Neal; Gisselbrecht, Stephen S.; Rusan, Nasser M.; Michelson, Alan M.

2012-01-01

SUMMARY The development of a complex organ requires the specification of appropriate numbers of each of its constituent cell types, as well as their proper differentiation and correct positioning relative to each other. During Drosophila cardiogenesis, all three of these processes are controlled by jumeau (jumu) and Checkpoint suppressor homologue (CHES-1-like), two genes encoding forkhead transcription factors that we discovered utilizing an integrated genetic, genomic and computational strategy for identifying genes expressed in the developing Drosophila heart. Both jumu and CHES-1-like are required during asymmetric cell division for the derivation of two distinct cardiac cell types from their mutual precursor, and in symmetric cell divisions that produce yet a third type of heart cell. jumu and CHES-1-like control the division of cardiac progenitors by regulating the activity of Polo, a kinase involved in multiple steps of mitosis. This pathway demonstrates how transcription factors integrate diverse developmental processes during organogenesis. PMID:22814603

A Small RNA-Catalytic Argonaute Pathway Tunes Germline Transcript Levels to Ensure Embryonic Divisions

PubMed Central

Gerson-Gurwitz, Adina; Wang, Shaohe; Sathe, Shashank; Green, Rebecca; Yeo, Gene W.; Oegema, Karen; Desai, Arshad

2016-01-01

SUMMARY Multiple division cycles without growth are a characteristic feature of early embryogenesis. The female germline loads proteins and RNAs into oocytes to support these divisions, which lack many quality control mechanisms operating in somatic cells undergoing growth. Here we describe a small RNA-Argonaute pathway that ensures early embryonic divisions in C. elegans by employing catalytic slicing activity to broadly tune, instead of silence, germline gene expression. Misregulation of one target, a kinesin-13 microtubule depolymerase, underlies a major phenotype associated with pathway loss. Tuning of target transcript levels is guided by density of homologous small RNAs, whose generation must ultimately be related to target sequence. Thus, the tuning action of a small RNA-catalytic Argonaute pathway generates oocytes capable of supporting embryogenesis. We speculate that the specialized nature of germline chromatin led to emergence of small RNA-catalytic Argonaute pathways in the female germline as a post-transcriptional control layer to optimize oocyte composition. PMID:27020753

Problems and potentialities of cultured plant cells in retrospect and prospect

NASA Technical Reports Server (NTRS)

Steward, F. C.; Krikorian, A. D.

1979-01-01

The past, present and expected future accomplishments and limitations of plant cell and tissue culture are reviewed. Consideration is given to the pioneering insights of Haberlandt in 1902, the development of culture techniques, and past work on cell division, cell and tissue growth and development, somatic embryogenesis, and metabolism and respiration. Current activity in culture media and technique development for plant regions, organs, tissues, cells, protoplasts, organelles and embryos, totipotency, somatic embryogenesis and clonal propagation under normal and space conditions, biochemical potentialities, and genetic engineering is surveyed. Prospects for the investigation of the induced control of somatic cell division, the division of isolated protoplasts, the improvement of haploid cell cultures, liquid cultures for somatic embryogenesis, and the genetic control of development are outlined.

3D Grain Reconstruction from Boxscan Data

DTIC Science & Technology

2010-01-01

H.F. Poulsen∗ and E. M. Lauridsen∗ ∗ Center for Fundamental Research: Metal Structures in Four Dimensions, Materials Research Division, Risø- DTU , DK...Center for Fundamental Research,Metal Structures in FOur Dimensions, Materials Research Division,Riso- DTU , DK-4000,Roskilde, Denmark, 8. PERFORMING...provision of beam time by the ESRF, Grenoble, France, is greatly acknowledged. Furthermore, the staff at Risø- DTU thanks R. Gardner for valuable

Proceedings of the Annual Meeting of the Association for Education in Journalism and Mass Communication (86th, Kansas City, Missouri, July 30-August 2, 2003). Mass Communication and Society Division.

ERIC Educational Resources Information Center

2003

The Mass Communication and Society Division of the proceedings contains the following 12 papers: "Free Congress Research and Education Foundation: An Extremist Organization in Think Tank Clothing?" (Sharron M. Hope); "Presence in Informative Virtual Environments: The Effects of Self-Efficacy, Spatial Ability and Mood" (Lynette…

Presentations - Herriott, T.M. and others, 2011 | Alaska Division of

Science.gov Websites

Details Title: Detailed geologic mapping and overview of structural and stratigraphic studies in the east Resident Business in Alaska Visiting Alaska State Employees DGGS State of Alaska search Alaska Division of in the east-central North Slope foothills, Alaska (poster): 3P Arctic, The Polar Petroleum Potential

National Program for Inspection of Non-Federal Dams. Highland Lakes-Lower Lake Dam (MA 00598), Connecticut River Basin, Goshen, Massachusetts. Phase I Inspection Report.

DTIC Science & Technology

1981-08-01

approval. CARNEY M. TERZLAN, KDIBER Design Branch Engineering Division Water\\ontrol Brancr%.J Engineering Division ARAiQAST MANTESI, CHIRA Geotechnical...issued to Hayden, Harding & Buchanan, Inc. on 26 June 1981 by William E. Hodgson Jr., Colonel, Corps of Engineers. Contract No. DACW 33-80-C-0006 has been

Publications - PDF 95-33A | Alaska Division of Geological & Geophysical

Science.gov Websites

the B-1 quadrangles, east-central Alaska Authors: Clough, J.G., Reifenstuhl, R.R., Mull, C.G., Pinney -1, C-1, and part of the B-1 quadrangles, east-central Alaska: Alaska Division of Geological & :63,360 (10.0 M) Sheet 2 Geologic map of the Charley River C-1 and part of the B-1 Quadrangle, east

Proceedings of the Annual Meeting of the Association for Education in Journalism and Mass Communication (83rd, Phoenix, Arizona, August 9-12, 2000). Advertising Division.

ERIC Educational Resources Information Center

Association for Education in Journalism and Mass Communication.

The Advertising Division section of the proceedings contains the following 14 papers: "The Effectiveness of Banner Advertisements: Involvement and Click-Through" (Chang-Hoan Cho and John D. Leckenby); "Messages of Hope: Developing Health Campaigns that Address Misperceptions of Breast Cancer Held by Women of Color" (Cynthia M.…

Proceedings of the Annual Meeting of the Association for Education in Journalism and Mass Communication (86th, Kansas City, Missouri, July 30-August 2, 2003). Miscellaneous Divisions.

ERIC Educational Resources Information Center

2003

The Miscellaneous Divisions of the proceedings contains the following 17 papers: "Analyzing Sequential Art: Visual Narrative Techniques in 'Calvin and Hobbes'" (Sharron M. Hope); "A Critical Vision of Gender in 2002 Campaign Ads" (Janis Teruggi Page); "Personal Impact Assessment of Advertising Culture of 'Whiteness':…

Pomegranate polyphenols and extract inhibit nuclear factor of activated T-cell activity and microglial activation in vitro and in a transgenic mouse model of Alzheimer disease.

PubMed

Rojanathammanee, Lalida; Puig, Kendra L; Combs, Colin K

2013-05-01

Alzheimer disease (AD) brain is characterized by extracellular plaques of amyloid β (Aβ) peptide with reactive microglia. This study aimed to determine whether a dietary intervention could attenuate microgliosis. Memory was assessed in 12-mo-old male amyloid precursor protein/presenilin 1 (APP/PS1) transgenic mice via Barnes maze testing followed by division into either a control-fed group provided free access to normal chow and water or a treatment group provided free access to normal chow and drinking water supplemented with pomegranate extract (6.25 mL/L) for 3 mo followed by repeat Barnes maze testing for both groups. Three months of pomegranate feeding decreased the path length to escape of mice compared with their initial 12-mo values (P < 0.05) and their control-fed counterparts (P < 0.05). Brains of the 3-mo study pomegranate-fed mice had lower tumor necrosis factor α (TNF-α) concentrations (P < 0.05) and lower nuclear factor of activated T-cell (NFAT) transcriptional activity (P < 0.05) compared with controls. Brains of the 3-mo pomegranate or control mice were also compared with an additional control group of 12-mo-old mice for histologic analysis. Immunocytochemistry showed that pomegranate- but not control-fed mice had attenuated microgliosis (P < 0.05) and Aβ plaque deposition (P < 0.05) compared with 12-mo-old mice. An additional behavioral study again used 12-mo-old male APP/PS1 mice tested by T-maze followed by division into a control group provided with free access to normal chow and sugar supplemented drinking water or a treatment group provided with normal chow and pomegranate extract-supplemented drinking water (6.25 mL/L) for 1 mo followed by repeat T-maze testing in both groups. One month of pomegranate feeding increased spontaneous alternations versus control-fed mice (P < 0.05). Cell culture experiments verified that 2 polyphenol components of pomegranate extract, punicalagin and ellagic acid, attenuated NFAT activity in a reporter cell line (P < 0.05) and decreased Aβ-stimulated TNF-α secretion by murine microglia (P < 0.05). These data indicate that dietary pomegranate produces brain antiinflammatory effects that may attenuate AD progression.

Pomegranate Polyphenols and Extract Inhibit Nuclear Factor of Activated T-Cell Activity and Microglial Activation In Vitro and in a Transgenic Mouse Model of Alzheimer Disease123

PubMed Central

Rojanathammanee, Lalida; Puig, Kendra L.; Combs, Colin K.

2013-01-01

Alzheimer disease (AD) brain is characterized by extracellular plaques of amyloid β (Aβ) peptide with reactive microglia. This study aimed to determine whether a dietary intervention could attenuate microgliosis. Memory was assessed in 12-mo-old male amyloid precursor protein/presenilin 1 (APP/PS1) transgenic mice via Barnes maze testing followed by division into either a control-fed group provided free access to normal chow and water or a treatment group provided free access to normal chow and drinking water supplemented with pomegranate extract (6.25 mL/L) for 3 mo followed by repeat Barnes maze testing for both groups. Three months of pomegranate feeding decreased the path length to escape of mice compared with their initial 12-mo values (P < 0.05) and their control-fed counterparts (P < 0.05). Brains of the 3-mo study pomegranate-fed mice had lower tumor necrosis factor α (TNF-α) concentrations (P < 0.05) and lower nuclear factor of activated T-cell (NFAT) transcriptional activity (P < 0.05) compared with controls. Brains of the 3-mo pomegranate or control mice were also compared with an additional control group of 12-mo-old mice for histologic analysis. Immunocytochemistry showed that pomegranate- but not control-fed mice had attenuated microgliosis (P < 0.05) and Aβ plaque deposition (P < 0.05) compared with 12-mo-old mice. An additional behavioral study again used 12-mo-old male APP/PS1 mice tested by T-maze followed by division into a control group provided with free access to normal chow and sugar supplemented drinking water or a treatment group provided with normal chow and pomegranate extract–supplemented drinking water (6.25 mL/L) for 1 mo followed by repeat T-maze testing in both groups. One month of pomegranate feeding increased spontaneous alternations versus control-fed mice (P < 0.05). Cell culture experiments verified that 2 polyphenol components of pomegranate extract, punicalagin and ellagic acid, attenuated NFAT activity in a reporter cell line (P < 0.05) and decreased Aβ-stimulated TNF-α secretion by murine microglia (P < 0.05). These data indicate that dietary pomegranate produces brain antiinflammatory effects that may attenuate AD progression. PMID:23468550

Interplay between CedA, rpoB and double stranded DNA: A step towards understanding CedA mediated cell division in E. coli.

PubMed

Sharma, Pankaj; Tomar, Anil Kumar; Kundu, Bishwajit

2018-02-01

Cell division is compromised in DnaAcos mutant E. coli cells due to chromosome over-replication. In these cells, CedA acts as a regulatory protein and initiates cell division by a hitherto unknown mechanism. CedA, a double stranded DNA binding protein, interacts with various subunits of RNA polymerase complex, including rpoB. To reveal how this concert between CedA, rpoB and DNA brings about cell division in E. coli, we performed biophysical and in silico analysis and obtained mechanistic insights. Interaction between CedA and rpoB was shown by circular dichroism spectrometry and in silico docking experiments. Further, CedA and rpoB were allowed to interact individually to a selected DNA and their binding was monitored by fluorescence spectroscopy. The binding constants of these interactions as determined by BioLayer Interferometry clearly show that rpoB binds to DNA with higher affinity (K D2 =<1.0E-12M) as compared to CedA (K D2 =9.58E-09M). These findings were supported by docking analysis where 12 intermolecular H-bonds were formed in rpoB-DNA complex as compared to 4 in CedA-DNA complex. Based on our data we propose that in E. coli cells chromosome over-replication signals CedA to recruit rpoB to specific DNA site(s), which initiates transcription of cell division regulatory elements. Copyright © 2017 Elsevier B.V. All rights reserved.

Gibberellin Produced in the Cotyledon Is Required for Cell Division during Tissue Reunion in the Cortex of Cut Cucumber and Tomato Hypocotyls1

PubMed Central

Asahina, Masashi; Iwai, Hiroaki; Kikuchi, Akira; Yamaguchi, Shinjiro; Kamiya, Yuji; Kamada, Hiroshi; Satoh, Shinobu

2002-01-01

Cucumber (Cucumis sativus) hypocotyls were cut to one-half of their diameter transversely, and morphological and histochemical analyses of the process of tissue reunion in the cortex were performed. Cell division in the cortex commenced 3 d after cutting, and the cortex was nearly fully united within 7 d. 4′,6-Diamidino-2-phenylindole staining and 5-bromo-2′-deoxyuridine labeling experiments indicate that nDNA synthesis occurred during this process. In addition, specific accumulation of pectic substances was observed in the cell wall of attached cells in the reunion region of the cortex. Cell division during tissue reunion was strongly inhibited when the cotyledon was removed. This inhibition was reversed by applying gibberellin (GA, 10−4 m GA3) to the apical tip of the cotyledon-less plant. Supporting this observation, cell division in the cortex was inhibited by treatment of the cotyledon with 10−4 m uniconazole-P (an inhibitor of GA biosynthesis), and this inhibition was also reversed by simultaneous application of GA. In contrast to the essential role of cotyledon, normal tissue reunion in cut hypocotyls was still observed when the shoot apex was removed. The requirement of GA for tissue reunion in cut hypocotyls was also evident in the GA-deficient gib-1 mutant of tomato (Lycopersicon esculentum). Our results suggest that GA, possibly produced in cotyledons, is essential for cell division in reuniting cortex of cut hypocotyls. PMID:12011351

Laboratory Studies on the Charging of Dust Grains in a Plasma

NASA Astrophysics Data System (ADS)

Xu, Wenjun

1993-01-01

The charging of dust grains by the surrounding plasma is studied in a dusty plasma device (DPD) (Xu, W., B. Song, R. L. Merlino, and N. D'Angelo, Rev. Sci. Instrum., 63, 5266, 1992). The dusty plasma device consists of a rotating-drum dust dispersal device used in conjunction with an existing Q-machine, to produce extended, steady state, magnetized plasma columns. The dust density in the dust chamber is controlled by the drum rotation speed. The device is capable of generating a dusty plasma in which as much as about 90% of the negative charge is attached to the dust grains of 1-10mu m size. Measurements of the dust parameter eta, the percentage of negative charge on free electrons in the dusty plasma, are presented. The dust parameter eta is found to depend on the rotational speed of the dust chamber, plasma density and the type and size of different dust. The dust parameter eta is calculated from a pair of Langmuir curves taken with and without dust under the same conditions. The operation of the dust chamber as described above has been confirmed by the agreement between the measurements of eta and the direct mechanical measurements consisting of weighing dust samples collected within the rotation dust chamber, at different rotation rates. By varying the ratio d/lambda_ {rm D} between the intergrain distance and the plasma Debye length, the effects predicted by Goertz and Ip (Goertz, C. K., and W-H. Ip, Geophys. Res. Lett., 11, 349, 1984), and subsequently reanalyzed in a more general fashion by Whipple et al. (Whipple, E. C., T. G. Northrop, and D. A. Mendis, J. Geophys. Rev., 90, 7405, 1985), as "isolated" dust grains become "closely packed" grains, have been demonstrated experimentally (Xu, W., N. D'Angelo, and R. L. Merlino, J. Geophys. Rev., 98, 7843, 1993). Similar results are presented and compared for two types of dust, kaolin and Al_2O _3, which have been studied in the experiment.

The Effects of a Sports Nutrition Education Intervention on Nutritional Status, Sport Nutrition Knowledge, Body Composition, and Performance during Off Season Training in NCAA Division I Baseball Players

PubMed Central

Rossi, Fabrício Eduardo; Landreth, Andrew; Beam, Stacey; Jones, Taylor; Norton, Layne; Cholewa, Jason Michael

2017-01-01

This study investigated the effects of a sport nutrition education intervention (SNEI) on dietary intake, knowledge, body composition, and performance in NCAA Division I baseball players. Resistance trained NCAA Division I baseball players (82.4 ± 8.2 kg; 1.83 ± 0.06 m; 13.7 ± 5 % body fat) participated in the study during 12 weeks of off-season training. Fifteen players volunteered for SNEI while 15 players matched for position served as controls (C) for body composition and performance. The nutrition intervention group (NI) received a 90 min SNEI encompassing energy intake (Kcal), carbohydrate (CHO), protein (PRO), fat, food sources, and hydration. Sport nutrition knowledge questionnaires were administered to NI pre and post. Nutritional status was determined by three-day dietary logs administered to NI pre and post. Body composition and performance (5-10-5 shuttle test, vertical jump, broad jump, 1 RM squat) were measured pre and post for C and NI. Knowledge increased in NI. Pro and fat, but not CHO intake increased in NI. FM decreased pre to post in NI (11.5 ± 4.8 vs. 10.5 ± 5.4 kg) but not C (11.3 ± 4.7 vs. 11.9 ± 4.5 kg). FFM increased pre to post with no differences between groups. The 5-10-5 shuttle times decreased significantly more in NI (4.58 ± 0.15 vs. 4.43 ± 0.13 sec) compared to C (4.56 ± 0.18 vs. 4.50 ± 0.16 sec). Jump and squat performance increased pre to post with no differences between groups. Our findings indicate that an off season SNEI is effective at improving sport nutrition knowledge and some, but not all, nutrient intakes and performance measures in Division I baseball players. Key points Sport nutrition education intervention increased nutritional knowledge and nutritional status. Sport nutrition education intervention reduced body fat percentage, total fat mass, 5-10-5 shuttle times, and trended towards greater increases in lean mass compared to controls. Both groups increased strength and jump ability similarly. PMID:28344452

Occludin Independently Regulates Permeability under Hydrostatic Pressure and Cell Division in Retinal Pigment Epithelial Cells

PubMed Central

Phillips, Brett E.; Cancel, Limary; Tarbell, John M.; Antonetti, David A.

2008-01-01

Purpose The aim of this study was to determine the function of the tight junction protein occludin in the control of permeability, under diffusive and hydrostatic pressures, and its contribution to the control of cell division in retinal pigment epithelium. Methods Occludin expression was inhibited in the human retinal pigment epithelial cell line ARPE-19 by siRNA. Depletion of occludin was confirmed by Western blot, confocal microscopy, and RT-PCR. Paracellular permeability of cell monolayers to fluorescently labeled 70 kDa dextran, 10 kDa dextran, and 467 Da tetramethylrhodamine (TAMRA) was examined under diffusive conditions or after the application of 10 cm H2O transmural pressure. Cell division rates were determined by tritiated thymidine incorporation and Ki67 immunoreactivity. Cell cycle inhibitors were used to determine whether changes in cell division affected permeability. Results Occludin depletion increased diffusive paracellular permeability to 467 Da TAMRA by 15%, and permeability under hydrostatic pressure was increased 50% compared with control. Conversely, depletion of occludin protein with siRNA did not alter diffusive permeability to 70 kDa and 10 kDa RITC-dextran, and permeability to 70 kDa dextran was twofold lower in occludin-depleted cells under hydrostatic pressure conditions. Occludin depletion also increased thymidine incorporation by 90% and Ki67-positive cells by 50%. Finally, cell cycle inhibitors did not alter the effect of occludin siRNA on paracellular permeability. Conclusions The data suggest that occludin regulates tight junction permeability in response to changes in hydrostatic pressure. Furthermore, these data suggest that occludin also contributes to the control of cell division, demonstrating a novel function for this tight junction protein. PMID:18263810

National Program for Inspection of Non-Federal Dams. Collins Company Upper Dam (CT 00674), Connecticut River Basin, Canton, Connecticut. Phase I Inspection Report.

DTIC Science & Technology

1979-07-01

Engineering Division p 0 CAR WE H FRZIAN, NENBER Design Branch Engineering Division J SEPE FIN~EGAN, JR.,CIV ater Control Branch * Engineering Division...Operator g. Purpose of Dam h. Design and Construction History i. Normal Operational Procedures 1.3 PERTINENT DATA ........................... 4 a...Tunnel i. Spillways j. Regulating Outlets SECTION 2: ENGINEERING DATA 2.1 DESIGN .............................. 9 a. Available Data b. Design Features c

Development of Trypanosoma (M.) theileri in tabanids.

PubMed

Böse, R; Heister, N C

1993-01-01

Thus far the life cycle of Trypanosoma (Megatrypanum) theileri has not been studied. We collected tabanids during the mass hatching, when only few tabanids are infected with trypanosomes. Tabanids were caught immediately after attacking a bait cow to serve as controls or after they had been allowed to engorge on the Trypanosoma (M.) theileri-infected cow. Tabanids were kept in the laboratory and used to study the developmental cycle of T. (M.) theileri in the tabanid gut. From day 1 to day 10 the presumably unfed controls and the engorged tabanids were dissected and cytological smears made from the mid- and hindgut. In total 2.6% (1/38) of the controls and 39% (23/59) of the engorged tabanids were positive for trypanosomes in the 1991 season. From day 1 to day 4 after engorgement trypanosomes were found in the midgut. Epimastigotes with a length of 29 microm on day 1 after infection multiplied by inequal division to form smaller epimastigotes of 26 microm on day 3. On day 4 morphologically indistinguishable trypanosomes of 21 microm total length were found in both mid- and hindgut. From day 5 to day 10 trypanosomes were found only in the hindgut in which the transformation to metacyclics was demonstrated, i.e., epimastigotes transformed to amastigote stages of 5 microm in total length.

47 CFR 1.2105 - Bidding application and certification procedures; prohibition of certain communications.

Code of Federal Regulations, 2014 CFR

2014-10-01

... include changes in ownership of the applicant that would constitute an assignment or transfer of control... Auctions and Spectrum Access Division, Wireless Telecommunications Bureau, by the most expeditious means... Auctions and Spectrum Access Division, Wireless Telecommunications Bureau, by the most expeditious means...

14 CFR 325.4 - State and local participation.

Code of Federal Regulations, 2010 CFR

2010-01-01

... the Documentary Services Division, unless the Department specifies another date. If no response is... point by filing in the Documentary Services Division, five copies of a document titled with the name of... questionnaire. (Approved by the Office of Management and Budget under control number 3024-0037) ...

Investigation of Performance of Concrete and Concreting Materials Exposed to Natural Weathering. Volume 1. Active Investigations. Revision

DTIC Science & Technology

1980-08-01

Ms. K. Mather, Messrs. B. Mather, J. M. Scanlon, B. R. Sullivan, R. V. Tye, Jr., E. E. McCoy, E. C. Roshore, H. T. Thornton, R. E. Black, D. Glass , D...Investigations, Phases D (CW R&D) Section 9: Passamaquoddy Tidal Power Project Section 10: Missouri River Division Program Section 1: Portland Blast-Furnace Slag ...Missouri River Division Props.m 3- by 4-1/2- by 16-in. beam 3 2 No? 1965 10 pack 5 Portland lst-Puiiace Slag 3-1/2- by 1-1/2- by 16-in. beam 108 66 May

Disorganization of cell division of methicillin-resistant Staphylococcus aureus by methanolic extract from Phyllanthus columnaris stem bark

DOE Office of Scientific and Technical Information (OSTI.GOV)

Adnalizawati, A. Siti Noor; Nazlina, I.; Yaacob, W. A.

The in vitro activity of methanolic extract from Phyllanthus columnaris stem bark was studied against Methicillin-resistant Staphylococcus aureus (MRSA) ATCC 43300 and MRSA BM1 (clinical strain) using time-kill curves in conjunction with scanning and transmission electron microscopy. The extract showed more markedly bactericidal activity in MRSA BM1 clinical strain within less than 4 h by 6.25-12.5 mg/mL and within 6 h by 1.56 mg/mL. Scanning electron microscopy of MRSA BM1 revealed distortion of cell whilst transmission electron microscopy revealed disruption in cell wall division.

Milestone Completion Report WBS 1.3.5.05 ECP/VTK-m FY17Q4 [MS-17/03-06] Key Reduce / Spatial Division / Basic Advect / Normals STDA05-4.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Moreland, Kenneth D.

The FY17Q4 milestone of the ECP/VTK-m project includes the completion of a key-reduce scheduling mechanism, a spatial division algorithm, an algorithm for basic particle advection, and the computation of smoothed surface normals. With the completion of this milestone, we are able to, respectively, more easily group like elements (a common visualization algorithm operation), provide the fundamentals for geometric search structures, provide the fundamentals for many flow visualization algorithms, and provide more realistic rendering of surfaces approximated with facets.

Disorganization of cell division of methicillin-resistant Staphylococcus aureus by methanolic extract from Phyllanthus columnaris stem bark

NASA Astrophysics Data System (ADS)

Adnalizawati, A. Siti Noor; Nazlina, I.; Yaacob, W. A.

2013-11-01

The in vitro activity of methanolic extract from Phyllanthus columnaris stem bark was studied against Methicillin-resistant Staphylococcus aureus (MRSA) ATCC 43300 and MRSA BM1 (clinical strain) using time-kill curves in conjunction with scanning and transmission electron microscopy. The extract showed more markedly bactericidal activity in MRSA BM1 clinical strain within less than 4 h by 6.25-12.5 mg/mL and within 6 h by 1.56 mg/mL. Scanning electron microscopy of MRSA BM1 revealed distortion of cell whilst transmission electron microscopy revealed disruption in cell wall division.

77 FR 2096 - Proposal Review Panel for Materials Research; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-13

... Engineering Center (MRSEC) at Massachusetts Institute of Technology (MIT) by the Division of Materials... 16, 2012 7:15 a.m.-8:15 a.m. Closed--Executive Session 8:15 a.m.-5 p.m. Open--Review of the MIT MRSEC... session 9 a.m.-10 a.m. Open--Review of the MIT MRSEC 10 a.m.-4:30 p.m. Closed--Executive Session, Report...

Cell-Division Behavior in a Heterogeneous Swarm Environment.

PubMed

Erskine, Adam; Herrmann, J Michael

2015-01-01

We present a system of virtual particles that interact using simple kinetic rules. It is known that heterogeneous mixtures of particles can produce particularly interesting behaviors. Here we present a two-species three-dimensional swarm in which a behavior emerges that resembles cell division. We show that the dividing behavior exists across a narrow but finite band of parameters and for a wide range of population sizes. When executed in a two-dimensional environment the swarm's characteristics and dynamism manifest differently. In further experiments we show that repeated divisions can occur if the system is extended by a biased equilibrium process to control the split of populations. We propose that this repeated division behavior provides a simple model for cell-division mechanisms and is of interest for the formation of morphological structure and to swarm robotics.

Cyclin D2 in the basal process of neural progenitors is linked to non-equivalent cell fates

PubMed Central

Tsunekawa, Yuji; Britto, Joanne M; Takahashi, Masanori; Polleux, Franck; Tan, Seong-Seng; Osumi, Noriko

2012-01-01

Asymmetric cell division plays an indispensable role during corticogenesis for producing new neurons while maintaining a self-renewing pool of apical progenitors. The cellular and molecular determinants favouring asymmetric division are not completely understood. Here, we identify a novel mechanism for generating cellular asymmetry through the active transportation and local translation of Cyclin D2 mRNA in the basal process. This process is regulated by a unique cis-regulatory sequence found in the 3′ untranslated region (3′UTR) of the mRNA. Unequal inheritance of Cyclin D2 protein to the basally positioned daughter cell with the basal process confers renewal of the apical progenitor after asymmetric division. Conversely, depletion of Cyclin D2 in the apically positioned daughter cell results in terminal neuronal differentiation. We demonstrate that Cyclin D2 is also expressed in the developing human cortex within similar domains, thus indicating that its role as a fate determinant is ancient and conserved. PMID:22395070

Quantifying cell turnover using CFSE data.

PubMed

Ganusov, Vitaly V; Pilyugin, Sergei S; de Boer, Rob J; Murali-Krishna, Kaja; Ahmed, Rafi; Antia, Rustom

2005-03-01

The CFSE dye dilution assay is widely used to determine the number of divisions a given CFSE labelled cell has undergone in vitro and in vivo. In this paper, we consider how the data obtained with the use of CFSE (CFSE data) can be used to estimate the parameters determining cell division and death. For a homogeneous cell population (i.e., a population with the parameters for cell division and death being independent of time and the number of divisions cells have undergone), we consider a specific biologically based "Smith-Martin" model of cell turnover and analyze three different techniques for estimation of its parameters: direct fitting, indirect fitting and rescaling method. We find that using only CFSE data, the duration of the division phase (i.e., approximately the S+G2+M phase of the cell cycle) can be estimated with the use of either technique. In some cases, the average division or cell cycle time can be estimated using the direct fitting of the model solution to the data or by using the Gett-Hodgkin method [Gett A. and Hodgkin, P. 2000. A cellular calculus for signal integration by T cells. Nat. Immunol. 1:239-244]. Estimation of the death rates during commitment to division (i.e., approximately the G1 phase of the cell cycle) and during the division phase may not be feasible with the use of only CFSE data. We propose that measuring an additional parameter, the fraction of cells in division, may allow estimation of all model parameters including the death rates during different stages of the cell cycle.

Task Division within the Prefrontal Cortex: Distinct Neuron Populations Selectively Control Different Aspects of Aggressive Behavior via the Hypothalamus.

PubMed

Biro, Laszlo; Sipos, Eszter; Bruzsik, Biborka; Farkas, Imre; Zelena, Dora; Balazsfi, Diana; Toth, Mate; Haller, Jozsef

2018-04-25

An important question in behavioral neurobiology is how particular neuron populations and pathways mediate the overall roles of brain structures. Here we investigated this issue by studying the medial prefrontal cortex (mPFC), an established locus of inhibitory control of aggression. We established in male rats that dominantly distinct mPFC neuron populations project to and produce dense fiber networks with glutamate release sites in the mediobasal hypothalamus (MBH) and lateral hypothalamus (LH; i.e., two executory centers of species-specific and violent bites, respectively). Optogenetic stimulation of mPFC terminals in MBH distinctively increased bite counts in resident/intruder conflicts, whereas the stimulation of similar terminals in LH specifically resulted in violent bites. No other behaviors were affected by stimulations. These findings show that the mPFC controls aggressiveness by behaviorally dedicated neuron populations and pathways, the roles of which may be opposite to those observed in experiments where the role of the whole mPFC (or of its major parts) has been investigated. Overall, our findings suggest that the mPFC organizes into working units that fulfill specific aspects of its wide-ranging roles. SIGNIFICANCE STATEMENT Aggression control is associated with many cognitive and emotional aspects processed by the prefrontal cortex (PFC). However, how the prefrontal cortex influences quantitative and qualitative aspects of aggressive behavior remains unclear. We demonstrated that dominantly distinct PFC neuron populations project to the mediobasal hypothalamus (MBH) and the lateral hypothalamus (LH; i.e., two executory centers of species-specific and violent bites, respectively). Stimulation of mPFC fibers in MBH distinctively increased bite counts during fighting, whereas stimulation of similar terminals in LH specifically resulted in violent bites. Overall, our results suggest a direct prefrontal control over the hypothalamus, which is involved in the modulation of quantitative and qualitative aspects of aggressive behavior through distinct prefrontohypothalamic projections. Copyright © 2018 the authors 0270-6474/18/384065-11$15.00/0.

Drosophila Sulf1 is required for the termination of intestinal stem cell division during regeneration.

PubMed

Takemura, Masahiko; Nakato, Hiroshi

2017-01-15

Stem cell division is activated to trigger regeneration in response to tissue damage. The molecular mechanisms by which this stem cell mitotic activity is properly repressed at the end of regeneration are poorly understood. Here, we show that a specific modification of heparan sulfate is crucial for regulating Drosophila intestinal stem cell (ISC) division during normal midgut homeostasis and regeneration. Loss of the extracellular heparan sulfate endosulfatase Sulf1 resulted in increased ISC division during normal homeostasis, which was caused by upregulation of mitogenic signaling including the JAK-STAT, EGFR and Hedgehog pathways. Using a regeneration model, we found that ISCs failed to properly halt division at the termination stage in Sulf1 mutants, showing that Sulf1 is required for terminating ISC division at the end of regeneration. We propose that post-transcriptional regulation of mitogen signaling by heparan sulfate structural modifications provides a new regulatory step for precise temporal control of stem cell activity during regeneration. © 2017. Published by The Company of Biologists Ltd.

Cyclin D regulation of a sexually dimorphic asymmetric cell division

PubMed Central

Tilmann, Christopher; Kimble, Judith

2006-01-01

SUMMARY The C. elegans somatic gonadal precursor cell (SGP) divides asymmetrically to establish gonad-specific coordinates in both sexes. In addition, the SGP division is sexually dimorphic and initiates sex-specific programs of gonadogenesis. Wnt/MAPK signaling determines the gonadal axes, and the FKH-6 transcription factor specifies the male mode of SGP division. In this paper, we demonstrate that C. elegans cyclin D controls POP-1/TCF asymmetry in the SGP daughters as well as fkh-6 and rnr expression in the SGPs. Although cyclin D mutants have delayed SGP divisions, the cyclin D defects are not mimicked by other methods of retarding the SGP division. We find that EFL-1/E2F has an antagonistic effect on fkh-6 expression and gonadogenesis, which is relieved by cyclin D activity. We propose that cyclin D and other canonical regulators of the G1/S transition coordinate key regulators of axis formation and sex determination with cell cycle progression to achieve the sexually dimorphic SGP asymmetric division. PMID:16198291

Drosophila Sulf1 is required for the termination of intestinal stem cell division during regeneration

PubMed Central

2017-01-01

ABSTRACT Stem cell division is activated to trigger regeneration in response to tissue damage. The molecular mechanisms by which this stem cell mitotic activity is properly repressed at the end of regeneration are poorly understood. Here, we show that a specific modification of heparan sulfate is crucial for regulating Drosophila intestinal stem cell (ISC) division during normal midgut homeostasis and regeneration. Loss of the extracellular heparan sulfate endosulfatase Sulf1 resulted in increased ISC division during normal homeostasis, which was caused by upregulation of mitogenic signaling including the JAK-STAT, EGFR and Hedgehog pathways. Using a regeneration model, we found that ISCs failed to properly halt division at the termination stage in Sulf1 mutants, showing that Sulf1 is required for terminating ISC division at the end of regeneration. We propose that post-transcriptional regulation of mitogen signaling by heparan sulfate structural modifications provides a new regulatory step for precise temporal control of stem cell activity during regeneration. PMID:27888216

High frame-rate resolution of cell division during Candida albicans filamentation

PubMed Central

Thomson, Darren D.; Berman, Judith; Brand, Alexandra C.

2016-01-01

The commensal yeast, Candida albicans, is an opportunistic pathogen in humans and forms filaments called hyphae and pseudohyphae, in which cell division requires precise temporal and spatial control to produce mononuclear cell compartments. High-frame-rate live-cell imaging (1 frame/min) revealed that nuclear division did not occur across the septal plane. We detected the presence of nucleolar fragments that may be extrachromosomal molecules carrying the ribosomal RNA genes. Cells occasionally maintained multiple nucleoli, suggesting either polyploidy, multiple nuclei and/or aneuploidy of ChrR., while the migration pattern of sister nuclei differed between unbranched and branched hyphae. The presented movie challenges and extends previous concepts of C. albicans cell division. PMID:26854071

77 FR 58871 - Proposal Review Panel for Materials Research; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-24

... Source (CHESS) at Cornell University by the Division of Materials Research (DMR) 1203 Dates & Times... and further support of the CHESS facility at Cornell. Agenda Sunday, October 28, 2012 5:45 p.m.-7 p.m. Closed--Executive Session 7 p.m.-8:30 p.m. Open--Tour of CHESS Monday, October 29, 2012 8 a.m.-4 p.m...

Human factors in telemanipulation: Perspectives from the Oak Ridge National Laboratory experience

DOE Office of Scientific and Technical Information (OSTI.GOV)

Draper, J.V.

1994-01-01

Personnel at the Robotics and Process Systems Division (RPSD) of the Oak Ridge National Laboratory (ORNL) have extensive experience designing, building, and operating teleoperators for a variety of settings, including space, battlefields, nuclear fuel reprocessing plants, and hazardous waste retrieval. In the course of the last decade and a half, the RPSD designed, built, and operated 4 telemanipulators (M-2, ASM, LTM, CESAR arm) and operated another half dozen (M-8, Model 50, TOS SM-229, RM-10, PaR 5000, BilArm 83A). During this period, human factors professionals have been closely integrated with RPSD design teams, investigating telemanipulator feedback and feed forward, designing cockpitsmore » and control rooms, training users and designers, and helping to develop performance specifications for telemanipulators. This paper presents a brief review of this and other work, with an aim towards providing perspectives on some of the human factors aspects of telemanipulation. The first section of the paper examines user tasks during supervisory control and discusses how telemanipulator responsiveness determines the appropriate control metaphor for continuous manual control. The second section provides an ecological perspective on telemanipulator feedback and feed-forward. The third section briefly describes the RPSD control room design approach and how design projects often serve as systems integrators.« less

77 FR 42501 - Agency Information Collection Activities; Submission for Office of Management and Budget Review...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-19

...] Agency Information Collection Activities; Submission for Office of Management and Budget Review; Comment Request; Guidance for Industry on Formal Dispute Resolution; Appeals Above the Division Level AGENCY: Food... the Division Level--(OMB Control Number 0910-0430)--(Extension) This information collection approval...

Monitoring and Controlling Engineering and Construction Management Cost Performance Within the Corps of Engineers

DTIC Science & Technology

1988-12-01

COST MANAGEMENT The CMIF approach addresses total costs but does not permit the analysis of indirect costs. We found that indirect costs vary...responsibility USACE/divisions Increasing CMIF Districts/divisions level of by fund type detail G&A, technical indirect, burden Districts by fund type

14 CFR Sec. 2-2 - Basis of allocation between entities.

Code of Federal Regulations, 2010 CFR

2010-01-01

... the air carrier, as well as each transport entity and organizational division of the air carrier for... recorded and placed initially under accounting controls of the particular air transport entity or organizational division of the air carrier or member of an affiliated group to which directly traceable. If...

21 CFR 1404.625 - What is the scope of a suspension or debarment?

Code of Federal Regulations, 2010 CFR

2010-04-01

...? 1404.625 Section 1404.625 Food and Drugs OFFICE OF NATIONAL DRUG CONTROL POLICY GOVERNMENTWIDE... debarment of all of your divisions and other organizational elements from all covered transactions, unless... identified individuals, divisions, or other organizational elements; or (2) To specific types of transactions...

77 FR 43368 - Joerns Healthcare, LLC, Stevens Point, Wisconsin Division, Including On-Site Leased Workers From...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-24

.... The Department has determined that these workers were sufficiently under the control of the subject..., Stevens Point, Wisconsin Division, Including On-Site Leased Workers From ABR, Aerotek, and Manpower, Stevens Point, WI; Amended Certification Regarding Eligibility To Apply for Worker Adjustment Assistance...

78 FR 52982 - Regal Beloit Corporation; Springfield, Missouri Division Including On-site Leased Workers From...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-27

... these workers were sufficiently under the control of the subject firm to be considered leased workers...; Springfield, Missouri Division Including On-site Leased Workers From Penmac Personnel Services and GCA Services Group Springfield, Missouri; Amended Certification Regarding Eligibility to Apply for Worker...

76 FR 4485 - Privacy Act of 1974; Report of Modified or Altered System of Records

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-25

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention Privacy Act of 1974; Report of Modified or Altered System of Records AGENCY: Division of Global Migration and... Infectious Diseases (CCID), Division of Global Migration and Quarantine, National Center for the Preparedness...

Leucine Modulation of the mTOR Pathway for Cognition Modulation: Kinetic and In Vitro Studies and Model Development

DTIC Science & Technology

2015-09-30

isoleucine non-polar L1 131 Aromatic side chain phenylalanine non-polar L1 165 tyrosine polar L1 181 tryptophan slightly...Human Performance Wing Human Effectiveness Directorate Bioeffects Division Molecular Bioeffects Branch Wright-Patterson AFB OH 45433-5707 NOTICE...IV, DAF Chief, Bioeffects Division Human Effectiveness Directorate 711th Human Performance Wing Air Force Research Laboratory This report is

IFLA General Conference, 1992. Division of Special Libraries: Section on Art Libraries; Section on Geography and Map Libraries; Section on Government Libraries; Section on Science and Technology Libraries. Papers.

ERIC Educational Resources Information Center

International Federation of Library Associations and Institutions, London (England).

The following 21 papers were delivered for the Special Libraries Division of the International Federation of Library Associations and Institutions at its 1992 annual meeting: (1) "From Indochina to Afghanistan: Arts from Abroad in Parisian Libraries" (M. F. Macouin); (2) "The Indonesian Archeology Photograph and Documentation System…

Near-Earth space hazards and their detection (Scientific session of the Physical Sciences Division of the Russian Academy of Sciences, 27 March 2013)

NASA Astrophysics Data System (ADS)

2013-08-01

A scientific session of the Physical Sciences Division of the Russian Academy of Sciences (RAS), titled "Near-Earth space hazards and their detection", was held on 27 March 2013 at the conference hall of the Lebedev Physical Institute, RAS. The agenda posted on the website of the Physical Sciences Division, RAS, http://www.gpad.ac.ru, included the following reports: (1) Emel'yanenko V V, Shustov B M (Institute of Astronomy, RAS, Moscow) "The Chelyabinsk event and the asteroid-comet hazard"; (2) Chugai N N (Institute of Astronomy, RAS, Moscow) "A physical model of the Chelyabinsk event"; (3) Lipunov V M (Lomonosov Moscow State University, Sternberg Astronomical Institute, Moscow) "MASTER global network of optical monitoring"; (4) Beskin G M (Special Astrophysical Observatory, RAS, Arkhyz, Karachai-Cirkassian Republic) "Wide-field optical monitoring systems with subsecond time resolution for the detection and study of cosmic threats". The expanded papers written on the base of oral reports 1 and 4 are given below. • The Chelyabinsk event and the asteroid-comet hazard, V V Emel'yanenko, B M Shustov Physics-Uspekhi, 2013, Volume 56, Number 8, Pages 833-836 • Wide-field subsecond temporal resolution optical monitoring systems for the detection and study of cosmic hazards, G M Beskin, S V Karpov, V L Plokhotnichenko, S F Bondar, A V Perkov, E A Ivanov, E V Katkova, V V Sasyuk, A Shearer Physics-Uspekhi, 2013, Volume 56, Number 8, Pages 836-842

An essential role for the RNA-binding protein Smaug during the Drosophila maternal-to-zygotic transition.

PubMed

Benoit, Beatrice; He, Chun Hua; Zhang, Fan; Votruba, Sarah M; Tadros, Wael; Westwood, J Timothy; Smibert, Craig A; Lipshitz, Howard D; Theurkauf, William E

2009-03-01

Genetic control of embryogenesis switches from the maternal to the zygotic genome during the maternal-to-zygotic transition (MZT), when maternal mRNAs are destroyed, high-level zygotic transcription is initiated, the replication checkpoint is activated and the cell cycle slows. The midblastula transition (MBT) is the first morphological event that requires zygotic gene expression. The Drosophila MBT is marked by blastoderm cellularization and follows 13 cleavage-stage divisions. The RNA-binding protein Smaug is required for cleavage-independent maternal transcript destruction during the Drosophila MZT. Here, we show that smaug mutants also disrupt syncytial blastoderm stage cell-cycle delays, DNA replication checkpoint activation, cellularization, and high-level zygotic expression of protein coding and micro RNA genes. We also show that Smaug protein levels increase through the cleavage divisions and peak when the checkpoint is activated and zygotic transcription initiates, and that transgenic expression of Smaug in an anterior-to-posterior gradient produces a concomitant gradient in the timing of maternal transcript destruction, cleavage cell cycle delays, zygotic gene transcription, cellularization and gastrulation. Smaug accumulation thus coordinates progression through the MZT.

Depositional environment of downdip Yegua (Eocene) sandstones, Jackson County, Texas

DOE Office of Scientific and Technical Information (OSTI.GOV)

Whitten, C.J.; Berg, R.R.

Yegua sandstones at a depth of 8300-8580 ft (2530-2615 m) were partly cored in the Arco Jansky 1 dry hole. Total thickness of the sandstone section is approximately 240 ft (73 m). The sandstones are enclosed in thick marine shales and are about 20 mi (32 km) downdip from thicker and more abundant sandstones in the Yegua Formation. The section is similar to reservoirs recently discovered in the area at the Toro Grande (1984), Lost Bridge (1984), and El Torito (1985) fields. The sandstones are fine to very fine grained and occur in thin beds that are 0.5-9 ft (0.15-2.7more » m) thick. Sedimentary structures within the beds range from a lower massive division to a laminated or rippled upper division. Grain size within beds fines upward from 0.18 mm at the base to 0.05 mm at the top. The sandstones are interpreted to be turbidites of the AB type that were deposited within channels. The sandstones contain an average of 50% quartz and are classified as volcanic-arenites to feldspathic litharenites. Carbonate cement ranges from 0 to 27%. Average porosity is 29% and permeabilities are in the range of 60-1600 md in the clean sandstones. Much of the porosity is secondary and is the result of the dissolution of cements, volcanic rock fragments, and feldspar grains. Yegua sandstones produce gas and condensate at nearby Toro Grande field on a gentle, faulted anticline. The local trend of reservoir sandstones may be controlled in part by faulting that was contemporaneous with deposition.« less

77 FR 27115 - Newport to Bermuda Regatta, Narragansett Bay, Newport, RI

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-09

... Bermuda Regatta, Narragansett Bay, Rhode Island, from 10 a.m. to 3:30 p.m. on Friday, June 15, 2012... will be enforced from 10 a.m. to 3:30 p.m. on June 15, 2012. FOR FURTHER INFORMATION CONTACT: Mr. Edward LeBlanc, Chief, Waterways Management Division, Sector Southeastern New England, (401) 435-2351...

The case of the downsizing decision.

PubMed

Train, A S

1991-01-01

New CEO Charles Rampart's decision to make deep across-the-board cuts at Universal Products Company, Ltd. presents division manager Andrew Jordan with a thorny problem. Plagued by slow growth, a declining stock price, and an increasingly skeptical investment community, UPC needs to control costs and control them fast. But Jordan's division is the most profitable in the company, and the 11% cut proposed by Rampart could destroy already shaky morale and seriously threaten the division's ability to compete. "There comes a time in every manager's career when he has to fight a bad decision made by his boss," argues Sam Godwyn, Jordan's vice president for marketing and sales. "To cut across the board is to take a blunt axe to the company when a surgeon's scalpel is called for." He suggests it is better to line up support for an alternative plan that links cuts to a long-term strategy and that differentiates between successful and unsuccessful divisions. "It would be a terrible mistake for us to focus only on the narrow needs of the division when the future of the whole company is at stake," counters Mary Wyatt, Jordan's vice president for finance. Yes, the downsizing will hurt the division in the short term, but the real issue is getting behind the new CEO. Supporting the downsizing decision is a necessary investment in this future credibility and effectiveness--whatever the short-term costs. Four commentators debate Jordan's dilemma and how he should resolve it.

Entanglement witness criteria of strong- k-separability for multipartite quantum states

NASA Astrophysics Data System (ADS)

Yan, Siqing; Hou, Jinchuan

2018-07-01

Let H1, H2,\\ldots ,Hn be separable complex Hilbert spaces with \\dim Hi≥ 2 and n≥ 2. Assume that ρ is a state in H=H_1⊗ H_2⊗ \\cdots ⊗ H_n. ρ is called strong- k-separable (2≤ k≤ n) if ρ is separable for any k-partite division of H. In this paper, an entanglement witnesses criterion of strong- k-separability is obtained, which says that ρ is not strong- k-separable if and only if there exist a k-division space H_{m1}⊗ \\cdots ⊗ H_{mk} of H, a finite-rank linear elementary operator positive on product states Λ: B(H_{m2}⊗ \\cdots ⊗ H_{mk})→ B(H_{m1}) and a state ρ 0\\in S(H_{m1}⊗ H_{m1}), such that Tr(Wρ )<0, where W=(Id⊗ Λ ^{\\dagger })ρ 0 is an entanglement witness. In addition, several different methods of constructing entanglement witnesses for multipartite states are also given.

78 FR 70953 - Draft Guidance for Industry on Bioequivalence Recommendations for Fluticasone Propionate...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-27

...), Food and Drug Administration, 7519 Standish Pl., Rockville, MD 20855, 240-276-8465. SUPPLEMENTARY... in the Division of Dockets Management between 9 a.m. and 4 p.m., Monday through Friday, and will be...

Publications - AR 1981 | Alaska Division of Geological & Geophysical

Science.gov Websites

Sheets Maps & Other Oversized Sheets Plate 1 Significant mineral deposits and prospects in Alaska (6.8 M) Plate 2 Major active claim blocks and development projects in Alaska, scale 1:42,240 (13.0 M

A local maximum in gibberellin levels regulates maize leaf growth by spatial control of cell division.

PubMed

Nelissen, Hilde; Rymen, Bart; Jikumaru, Yusuke; Demuynck, Kirin; Van Lijsebettens, Mieke; Kamiya, Yuji; Inzé, Dirk; Beemster, Gerrit T S

2012-07-10

Plant growth rate is largely determined by the transition between the successive phases of cell division and expansion. A key role for hormone signaling in determining this transition was inferred from genetic approaches and transcriptome analysis in the Arabidopsis root tip. We used the developmental gradient at the maize leaf base as a model to study this transition, because it allows a direct comparison between endogenous hormone concentrations and the transitions between dividing, expanding, and mature tissue. Concentrations of auxin and cytokinins are highest in dividing tissues, whereas bioactive gibberellins (GAs) show a peak at the transition zone between the division and expansion zone. Combined metabolic and transcriptomic profiling revealed that this GA maximum is established by GA biosynthesis in the division zone (DZ) and active GA catabolism at the onset of the expansion zone. Mutants defective in GA synthesis and signaling, and transgenic plants overproducing GAs, demonstrate that altering GA levels specifically affects the size of the DZ, resulting in proportional changes in organ growth rates. This work thereby provides a novel molecular mechanism for the regulation of the transition from cell division to expansion that controls organ growth and size. Copyright © 2012 Elsevier Ltd. All rights reserved.

Uhrf1 controls the self-renewal versus differentiation of hematopoietic stem cells by epigenetically regulating the cell-division modes

PubMed Central

Zhao, Jingyao; Chen, Xufeng; Song, Guangrong; Zhang, Jiali; Liu, Haifeng; Liu, Xiaolong

2017-01-01

Hematopoietic stem cells (HSCs) are able to both self-renew and differentiate. However, how individual HSC makes the decision between self-renewal and differentiation remains largely unknown. Here we report that ablation of the key epigenetic regulator Uhrf1 in the hematopoietic system depletes the HSC pool, leading to hematopoietic failure and lethality. Uhrf1-deficient HSCs display normal survival and proliferation, yet undergo erythroid-biased differentiation at the expense of self-renewal capacity. Notably, Uhrf1 is required for the establishment of DNA methylation patterns of erythroid-specific genes during HSC division. The expression of these genes is enhanced in the absence of Uhrf1, which disrupts the HSC-division modes by promoting the symmetric differentiation and suppressing the symmetric self-renewal. Moreover, overexpression of one of the up-regulated genes, Gata1, in HSCs is sufficient to phenocopy Uhrf1-deficient HSCs, which show impaired HSC symmetric self-renewal and increased differentiation commitment. Taken together, our findings suggest that Uhrf1 controls the self-renewal versus differentiation of HSC through epigenetically regulating the cell-division modes, thus providing unique insights into the relationship among Uhrf1-mediated DNA methylation, cell-division mode, and HSC fate decision. PMID:27956603

Speed and countermovement-jump characteristics of elite female soccer players, 1995-2010.

PubMed

Haugen, Thomas A; Tønnessen, Espen; Seiler, Stephen

2012-12-01

The purpose of this investigation was to compare sprint and countermovement-jump (CMJ) performance among female competitive soccer players as a function of performance level, field position, and age. In addition, the authors wanted to quantify the evolution of these physical characteristics among elite players over a 15-y period. 194 female elite players (22± 4.1 y, 63 ± 5.6 kg), including an Olympic winning squad, tested 40-m sprint with electronic timing and CMJ on a force platform at the Norwegian Olympic training center from 1995 to 2010. Moderate to large velocity differences across performance levels and positions were observed. National-team players were 2% faster than 1st-division players (P = .027, d = 0.5) and 5% faster than 2nd-division players (P < .001, d = 1.3) over 0-20 m. National-team players jumped 8-9% higher than 1st-division players (P = .001, d = 0.6) and junior elite players (P = .023, d = 0.5). Forwards were 3-4% faster than midfielders (P < .001, d = 0.8) and goalkeepers (P = .003, d = 0.9) over 0-20 m. No differences in velocity or CMJ height were observed among the age categories. Players from 2006-2010 were 2% faster (P < .05, d = 0.6) than players from 1995-1999 over 20 m, whereas no differences in 20- to 40-m velocity or CMJ performance were observed. This study provides effect-magnitude estimates for the influence of performance level, age, and player position on sprint and CMJ performance in female soccer players. While 20- to 40-m velocity and CMJ performance have remained stable over the time, there has been a moderate but positive development in 0- to 20-m velocity among elite performers.

Arrest of cell cycle by inhibition of ribonucleotide reductase induces accumulation of NAD+ by Mn2+-supplemented growth of Corynebacterium ammoniagenes.

PubMed

Abbouni, Bouziane; Elhariry, Hesham M; Auling, Georg

2003-01-01

Cell division of the wild type strain Corynebacterium (formerly Brevibacterium) ammoniagenes ATCC 6872 which requires 1 microM Mn2+ for balanced growth was inhibited by addition of 20 mM hydroxyurea (HU) or 10 mM p-methoxyphenol (MP) to a Mn2+-supplemented fermentation medium at an appropriate time. Scanning electron microscopy (SEM) showed a restricted elongation characteristic of arrest of the cell cycle in coryneform bacteria. The cultures treated with HU or MP had, respectively, a fourfold or sixfold enhanced accumulation of NAD+ by a salvage biosynthetic pathway. An assay of nucleotide-permeable cells for ribonucleotide reductase activity using [3H-CDP] as substrate revealed a pre-early and complete decline of DNA precursor biosynthesis not found in the untreated control. Overproduction of NAD+ is an alternative to the conventional fermentation process using Mn2+ deficiency. A simple model is presented to discuss the metabolic regulation of the new process based on the presence of a manganese ribonucleotide reductase (Mn-RNR) in the producing strain.

Mitosis-Specific Mechanosensing and Contractile Protein Redistribution Control Cell Shape

PubMed Central

Effler, Janet C.; Kee, Yee-Seir; Berk, Jason M.; Tran, Minhchau N.; Iglesias, Pablo A.; Robinson, Douglas N.

2008-01-01

Summary Because cell division failure is deleterious, promoting tumorigenesis in mammals [1], cells utilize numerous mechanisms to control their cell-cycle progression [2–4]. Though cell division is considered a well-ordered sequence of biochemical events [5], cytokinesis, an inherently mechanical process, must also be mechanically controlled to ensure that two equivalent daughter cells are produced with high fidelity. Since cells respond to their mechanical environment [6, 7], we hypothesized that cells utilize mechanosensing and mechanical feedback to sense and correct shape asymmetries during cytokinesis. Because the mitotic spindle and myosin-II are vital to cell division [8, 9], we explored their roles in responding to shape perturbations during cell division. We demonstrate that the contractile proteins, myosin-II and cortexillin-I, redistribute in response to intrinsic and externally induced shape asymmetries. In early cytokinesis, mechanical load overrides spindle cues and slows cytokinesis progression while contractile proteins accumulate and correct shape asymmetries. In late cytokinesis, mechanical perturbation also directs contractile proteins but without apparently disrupting cytokinesis. Significantly, this response only occurs during anaphase through cytokinesis, does not require microtubules, is independent of spindle orientation, but is dependent on myosin-II. Our data provide evidence for a mechanosensory system that directs contractile proteins to regulate cell shape during mitosis. PMID:17027494

An Autonomous Satellite Time Synchronization System Using Remotely Disciplined VC-OCXOs.

PubMed

Gu, Xiaobo; Chang, Qing; Glennon, Eamonn P; Xu, Baoda; Dempseter, Andrew G; Wang, Dun; Wu, Jiapeng

2015-07-23

An autonomous remote clock control system is proposed to provide time synchronization and frequency syntonization for satellite to satellite or ground to satellite time transfer, with the system comprising on-board voltage controlled oven controlled crystal oscillators (VC-OCXOs) that are disciplined to a remote master atomic clock or oscillator. The synchronization loop aims to provide autonomous operation over extended periods, be widely applicable to a variety of scenarios and robust. A new architecture comprising the use of frequency division duplex (FDD), synchronous time division (STDD) duplex and code division multiple access (CDMA) with a centralized topology is employed. This new design utilizes dual one-way ranging methods to precisely measure the clock error, adopts least square (LS) methods to predict the clock error and employs a third-order phase lock loop (PLL) to generate the voltage control signal. A general functional model for this system is proposed and the error sources and delays that affect the time synchronization are discussed. Related algorithms for estimating and correcting these errors are also proposed. The performance of the proposed system is simulated and guidance for selecting the clock is provided.

Coordination of size-control, reproduction and generational memory in freshwater planarians

NASA Astrophysics Data System (ADS)

Yang, Xingbo; Kaj, Kelson; Schwab, David; Collins, Eva-Maria

Uncovering the mechanisms that control size, growth, and division rates of systems reproducing through binary division means understanding basic principles of their life cycle. Recent work has focused on how division rates are regulated in bacteria and yeast, but this question has not yet been addressed in more complex, multicellular organisms. We have acquired a unique large-scale data set on the growth and asexual reproduction of two freshwater planarian species, Dugesia japonica and Dugesia tigrina, which reproduce by transverse fission and succeeding regeneration of head and tail pieces into new worms. We developed a new additive theoretical model that mixes multiple size control strategies based on worm size, growth, and waiting time. Our model quantifies the proportions of each strategy in the mixed dynamics, revealing the ability of the two planarian species to utilize different strategies in a coordinated manner for size control. Additionally, we found that head and tail offspring of both species employ different mechanisms to monitor and trigger their reproduction cycles. Finally, we show that generation-dependent memory effects in planarians need to be taken into account to accurately capture the experimental data.

HEU Holdup Measurements on 321-M A-Lathe

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dewberry, R.A.

The Analytical Development Section of SRTC was requested by the Facilities Disposition Division (FDD) of the Savannah River Site to determine the holdup of enriched uranium in the 321-M facility as part of an overall deactivation project of the facility. The 321-M facility was used to fabricate enriched uranium fuel assemblies, lithium-aluminum target tubes, neptunium assemblies, and miscellaneous components for the production reactors. The results of the holdup assays are essential for determining compliance with the solid waste Waste Acceptance Criteria, Material Control and Accountability, and to meet criticality safety controls. Three measurement systems were used to determine highly enrichedmore » uranium (HEU) holdup. This report covers holdup measurements on the A-Lathe that was used to machine uranium-aluminum-alloy (U-Al). Our results indicated that the lathe contained more than the limits stated in the Waste Acceptance Criteria (WAC) for the solid waste E-Area Vaults. Thus the lathe was decontaminated three times and assayed four times in order to bring the amounts of uranium to an acceptable content. This report will discuss the methodology, Non-Destructive Assay (NDA) measurements, and results of the U-235 holdup on the lathe.« less

Clumping in the Cassini Division and C Ring: Constraints from Stellar Occultations

NASA Astrophysics Data System (ADS)

Colwell, J. E.; Jerousek, R. G.; Esposito, L. W.

2014-12-01

Particles in Saturn's rings are engaged in a constant tug-of-war between interparticle gravitational and adhesive forces that lead to clumping, on the one hand, and Keplerian shear that inhibits accretion on the other. Depending on the surface mass density of the rings and the local orbital velocity, ephemeral clumps or self-gravity wakes can form, giving the rings granularity on the scale of the most-unstable length scale against gravitational collapse. The A ring and many regions of the B ring are dominated by self-gravity wakes with a typical radial wavelength of ~50-100 m. A characteristic of self-gravity wakes is that they can effectively shadow the relatively empty spaces in between them, depending on viewing geometry. This leads to geometry-dependent measurements of optical depth in occultations of the rings. The C ring and Cassini Division have significantly lower surface mass densities than the A and B ring such that in most of these regions the most-unstable wavelength is comparable to the size of the ring particles (~1 m) so that self-gravity wake formation is not expected nor have its characteristics in various measurements been observed. Here we present measurements of the optical depth of the C ring and Cassini Division with the Cassini Ultraviolet Imaging Spectrograph (UVIS) showing variations with viewing geometry in the "ramp" regions and the Cassini Division "triple band". These variations are characteristic of self-gravity wakes. We place limits on clumping in other regions of the C ring and Cassini Division.

Grain Filling Characteristics and Their Relations with Endogenous Hormones in Large- and Small-Grain Mutants of Rice.

PubMed

Zhang, Weiyang; Cao, Zhuanqin; Zhou, Qun; Chen, Jing; Xu, Gengwen; Gu, Junfei; Liu, Lijun; Wang, Zhiqin; Yang, Jianchang; Zhang, Hao

2016-01-01

This study determined if the variation in grain filling parameters between two different spikelet types of rice (Oryza sativa L.) is regulated by the hormonal levels in the grains. Two rice mutants, namely, a large-grain mutant (AZU-M) and a small-grain mutant (ZF802-M), and their respective wild types (AZU-WT and ZF802-WT) were grown in the field. The endosperm cell division rate, filling rate, and hormonal levels: zeatin + zeatin riboside (Z+ZR), indo-3-acetic acid (IAA), polyamines (PAs), and abscisic acid (ABA) were determined. The results showed that there was no significant difference between the filling and endosperm cell division rates. These rates were synchronous between the superior and inferior spikelets for both mutants. However, the abovementioned parameters were significantly different between the two spikelet types for the two wild types. The superior spikelets filled faster and their filling rate was higher compared to the inferior ones. Changes in the concentrations of plant hormones were consistent with the observed endosperm cell division rate and the filling rate for both types of spikelets of mutant and wild type plants. Regression analysis showed a significant positive correlation between cell division and filling rates with the concentrations of the investigated hormones. Exogenous chemical application verified the role of ABA, IAA, and PAs in grain filling. The results indicate that poor filling of inferior spikelets in rice occurs primarily due to the reduced hormone concentrations therein, leading to lower division rate of endosperm cells, fewer endosperm cells, slower filling rate, and smaller grain weight.

Grain Filling Characteristics and Their Relations with Endogenous Hormones in Large- and Small-Grain Mutants of Rice

PubMed Central

Zhang, Weiyang; Cao, Zhuanqin; Zhou, Qun; Chen, Jing; Xu, Gengwen; Gu, Junfei; Liu, Lijun; Wang, Zhiqin; Yang, Jianchang; Zhang, Hao

2016-01-01

This study determined if the variation in grain filling parameters between two different spikelet types of rice (Oryza sativa L.) is regulated by the hormonal levels in the grains. Two rice mutants, namely, a large-grain mutant (AZU-M) and a small-grain mutant (ZF802-M), and their respective wild types (AZU-WT and ZF802-WT) were grown in the field. The endosperm cell division rate, filling rate, and hormonal levels: zeatin + zeatin riboside (Z+ZR), indo-3-acetic acid (IAA), polyamines (PAs), and abscisic acid (ABA) were determined. The results showed that there was no significant difference between the filling and endosperm cell division rates. These rates were synchronous between the superior and inferior spikelets for both mutants. However, the abovementioned parameters were significantly different between the two spikelet types for the two wild types. The superior spikelets filled faster and their filling rate was higher compared to the inferior ones. Changes in the concentrations of plant hormones were consistent with the observed endosperm cell division rate and the filling rate for both types of spikelets of mutant and wild type plants. Regression analysis showed a significant positive correlation between cell division and filling rates with the concentrations of the investigated hormones. Exogenous chemical application verified the role of ABA, IAA, and PAs in grain filling. The results indicate that poor filling of inferior spikelets in rice occurs primarily due to the reduced hormone concentrations therein, leading to lower division rate of endosperm cells, fewer endosperm cells, slower filling rate, and smaller grain weight. PMID:27780273

Differences in cytokinin control on cellular dynamics of zucchini cotyledons cultivated in two experimental systems.

PubMed

Stoynova-Bakalova, E; Petrov, P; Gigova, L; Ivanova, N

2011-01-01

The effect of endogenous cytokinins on the pattern of palisade cell division post-germination does not depend on the conditions of cotyledon development -in planta (attached to seedlings) or in vitro (isolated from dry zucchini seeds and cultured on water). In cotyledons originating from 4-day-old seedlings (experimental system 1), exogenous cytokinin temporarily (in the first 2 day of cultivation) enhanced post-mitotic cell enlargement of palisade cells, mainly due to enhanced water uptake and use of cell storage compounds, all of which lead to cotyledon senescence. Cytokinin is not able to resume the completed palisade cell division on day 5. As a result, the number of cells and the final areas of treated and control cotyledons are quite similar. By contrast, the effects of cytokinin on cotyledons isolated from dry seeds (experimental system 2) are better expressed, promoting an increase in number of palisade cells accompanied by additional cotyledon area enlargement. However, the prolonged post-mitotic cell expansion in control cotyledons compensates for the reduced speed of cell growth and division activity and decreases differences in final cotyledon area between treatments. The results define cell division as the primary target of cytokinin stimulation in cotyledon tissues competent for division, and determine the temporal patterns of palisade cell cycling related to cotyledon age. This knowledge permits a better choice of experimental system to study effects on cell proliferation and cell growth, as well as cell enlargement and senescence-related events using physiologically homogeneous material. © 2010 German Botanical Society and The Royal Botanical Society of the Netherlands.

Multiple Duties for Spindle Assembly Checkpoint Kinases in Meiosis

PubMed Central

Marston, Adele L.; Wassmann, Katja

2017-01-01

Cell division in mitosis and meiosis is governed by evolutionary highly conserved protein kinases and phosphatases, controlling the timely execution of key events such as nuclear envelope breakdown, spindle assembly, chromosome attachment to the spindle and chromosome segregation, and cell cycle exit. In mitosis, the spindle assembly checkpoint (SAC) controls the proper attachment to and alignment of chromosomes on the spindle. The SAC detects errors and induces a cell cycle arrest in metaphase, preventing chromatid separation. Once all chromosomes are properly attached, the SAC-dependent arrest is relieved and chromatids separate evenly into daughter cells. The signaling cascade leading to checkpoint arrest depends on several protein kinases that are conserved from yeast to man. In meiosis, haploid cells containing new genetic combinations are generated from a diploid cell through two specialized cell divisions. Though apparently less robust, SAC control also exists in meiosis. Recently, it has emerged that SAC kinases have additional roles in executing accurate chromosome segregation during the meiotic divisions. Here, we summarize the main differences between mitotic and meiotic cell divisions, and explain why meiotic divisions pose special challenges for correct chromosome segregation. The less-known meiotic roles of the SAC kinases are described, with a focus on two model systems: yeast and mouse oocytes. The meiotic roles of the canonical checkpoint kinases Bub1, Mps1, the pseudokinase BubR1 (Mad3), and Aurora B and C (Ipl1) will be discussed. Insights into the molecular signaling pathways that bring about the special chromosome segregation pattern during meiosis will help us understand why human oocytes are so frequently aneuploid. PMID:29322045

Travel guidance system for vehicles

DOE Office of Scientific and Technical Information (OSTI.GOV)

Takanabe, K.; Yamamoto, M.; Ito, K.

1987-02-24

A travel guidance system is described for vehicles including: a heading sensor for detecting a direction of movement of a vehicle; a distance sensor for detecting a distance traveled by the vehicle; a map data storage medium preliminarily storing map data; a control unit for receiving a heading signal from the heading sensor and a distance signal from the distance sensor to successively compute a present position of the vehicle and for generating video signals corresponding to display data including map data from the map data storage medium and data of the present position; and a display having first andmore » second display portions and responsive to the video signals from the control unit to display on the first display portion a map and a present portion mark, in which: the map data storage medium comprises means for preliminarily storing administrative division name data and landmark data; and the control unit comprises: landmark display means for: (1) determining a landmark closest to the present position, (2) causing a position of the landmark to be displayed on the map and (3) retrieving a landmark massage concerning the landmark from the storage medium to cause the display to display the landmark message on the second display portion; division name display means for retrieving the name of an administrative division to which the present position belongs from the storage medium and causing the display to display a division name message on the second display portion; and selection means for selectively actuating at least one of the landmark display means and the division name display means.« less

Coordination of size-control, reproduction and generational memory in freshwater planarians

NASA Astrophysics Data System (ADS)

Yang, Xingbo; Kaj, Kelson J.; Schwab, David J.; Collins, Eva-Maria S.

2017-06-01

Uncovering the mechanisms that control size, growth, and division rates of organisms reproducing through binary division means understanding basic principles of their life cycle. Recent work has focused on how division rates are regulated in bacteria and yeast, but this question has not yet been addressed in more complex, multicellular organisms. We have, over the course of several years, assembled a unique large-scale data set on the growth and asexual reproduction of two freshwater planarian species, Dugesia japonica and Girardia tigrina, which reproduce by transverse fission and succeeding regeneration of head and tail pieces into new planarians. We show that generation-dependent memory effects in planarian reproduction need to be taken into account to accurately capture the experimental data. To achieve this, we developed a new additive model that mixes multiple size control strategies based on planarian size, growth, and time between divisions. Our model quantifies the proportions of each strategy in the mixed dynamics, revealing the ability of the two planarian species to utilize different strategies in a coordinated manner for size control. Additionally, we found that head and tail offspring of both species employ different mechanisms to monitor and trigger their reproduction cycles. Thus, we find a diversity of strategies not only between species but between heads and tails within species. Our additive model provides two advantages over existing 2D models that fit a multivariable splitting rate function to the data for size control: firstly, it can be fit to relatively small data sets and can thus be applied to systems where available data is limited. Secondly, it enables new biological insights because it explicitly shows the contributions of different size control strategies for each offspring type.

Coordination of size-control, reproduction and generational memory in freshwater planarians.

PubMed

Yang, Xingbo; Kaj, Kelson J; Schwab, David J; Collins, Eva-Maria S

2017-05-23

Uncovering the mechanisms that control size, growth, and division rates of organisms reproducing through binary division means understanding basic principles of their life cycle. Recent work has focused on how division rates are regulated in bacteria and yeast, but this question has not yet been addressed in more complex, multicellular organisms. We have, over the course of several years, assembled a unique large-scale data set on the growth and asexual reproduction of two freshwater planarian species, Dugesia japonica and Girardia tigrina, which reproduce by transverse fission and succeeding regeneration of head and tail pieces into new planarians. We show that generation-dependent memory effects in planarian reproduction need to be taken into account to accurately capture the experimental data. To achieve this, we developed a new additive model that mixes multiple size control strategies based on planarian size, growth, and time between divisions. Our model quantifies the proportions of each strategy in the mixed dynamics, revealing the ability of the two planarian species to utilize different strategies in a coordinated manner for size control. Additionally, we found that head and tail offspring of both species employ different mechanisms to monitor and trigger their reproduction cycles. Thus, we find a diversity of strategies not only between species but between heads and tails within species. Our additive model provides two advantages over existing 2D models that fit a multivariable splitting rate function to the data for size control: firstly, it can be fit to relatively small data sets and can thus be applied to systems where available data is limited. Secondly, it enables new biological insights because it explicitly shows the contributions of different size control strategies for each offspring type.

CONFOCAL MICROSCOPY SYSTEM PERFORMANCE: QA TESTS, QUANTITATION AND SPECTROSCOPY

EPA Science Inventory

Confocal Microscopy System Performance: QA tests, Quantitation and Spectroscopy.

Robert M. Zucker 1 and Jeremy M. Lerner 2,
1Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, Office of Research Development, U.S. Environmen...

Multi-Variant/Capability Next Generation Troop Seat (M-V/C NGTS)

DTIC Science & Technology

2009-01-01

John Plaga , Work Unit Manager MARK M. HOFFMAN Deputy Chief Biomechanics Branch Biosciences and Protection Division Human...John A. Plaga a. REPORT U b. ABSTRACT U c. THIS PAGE U SAR 20 19b. TELEPHONE NUMBER (include area

75 FR 157 - National Institute on Deafness and Other Communication Disorders; Notice of Closed Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-04

... Grant. Date: January 28, 2010. Time: 11 a.m. to 2 p.m. Agenda: To review and evaluate grant applications... Call.) Contact Person: Sheo Singh, PhD, Scientific Review Officer, Scientific Review Branch, Division...

Publications - PIR 2008-1E | Alaska Division of Geological & Geophysical

Science.gov Websites

River--Evidence for a thermogenic origin Authors: Decker, P.L., and Wartes, M.A. Publication Date: Mar thermogenic origin, in Wartes, M.A., and Decker, P.L., eds., Preliminary results of recent geologic field

78 FR 19535 - Proposal Review Panel for Materials Research; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-01

... Source (CHESS) at Cornell University by the Division of Materials Research (DMR) 1203 Dates & Times... and further support of the CHESS facility at Cornell. Agenda: Sunday, April 28, 2013 5:45 p.m.-7:15 p...:00 a.m.-3:00 p.m. Open--Review of CHESS 3:00 p.m.-4:40 p.m. Closed--Executive session 4:40 p.m.-8:00...

Cell cycles and cell division in the archaea.

PubMed

Samson, Rachel Y; Bell, Stephen D

2011-06-01

Until recently little was known about the cell cycle parameters and division mechanisms of archaeal organisms. Although this is still the case for the majority of archaea, significant advances have been made in some model species. The information that has been gleaned thus far points to a remarkable degree of diversity within the archaeal domain of life. More specifically, members of distinct phyla have very different chromosome copy numbers, replication control systems and even employ distinct machineries for cell division. Copyright © 2011 Elsevier Ltd. All rights reserved.

A History of the Acoustics Division of the Naval Research Laboratory: The First Eight Decades 1923 - 2008

DTIC Science & Technology

2013-08-01

Superintendent” goes back to the early days of NRL in the 1920s and has been retained to the present, refer- ring to the director of each NRL research division...installation, energy for the final amplifiers was stored essentially in the fuel tank of a gas- turbine -driven generator. Signifi- cant research in the Sound...Division found a method for control of the gas turbine which permitted it to supply power in long pulses without damage to the turbine . Research on

How PI3K-derived lipids control cell division.

PubMed

Campa, Carlo C; Martini, Miriam; De Santis, Maria C; Hirsch, Emilio

2015-01-01

To succeed in cell division, intense cytoskeletal and membrane remodeling are required to allow accurate chromosome segregation and cytoplasm partitioning. Spatial restriction of the actin dynamics and vesicle trafficking define the cell symmetry and equivalent membrane scission events, respectively. Protein complexes coordinating mitosis are recruited to membrane microdomains characterized by the presence of the phosphatidylinositol lipid members (PtdIns), like PtdIns(3,4,5)P 3,PtdIns(4,5)P 2, and PtdIns(3)P. These PtdIns represent a minor component of cell membranes, defining membrane domain identity, ultimately controlling cytoskeleton and membrane dynamics during mitosis. The coordinated presence of PtdIns(3,4,5)P 3 at the cell poles and PtdIns(4,5)P 2 at the cleavage furrow controls the polarity of the actin cytoskeleton leading to symmetrical cell division. In the endosomal compartment, the trafficking of PtdIns(3)P positive vesicles allows the recruitment of the protein machinery required for the abscission.

Evaluation of selective control information detection scheme in orthogonal frequency division multiplexing-based radio-over-fiber and visible light communication links

NASA Astrophysics Data System (ADS)

Dalarmelina, Carlos A.; Adegbite, Saheed A.; Pereira, Esequiel da V.; Nunes, Reginaldo B.; Rocha, Helder R. O.; Segatto, Marcelo E. V.; Silva, Jair A. L.

2017-05-01

Block-level detection is required to decode what may be classified as selective control information (SCI) such as control format indicator in 4G-long-term evolution systems. Using optical orthogonal frequency division multiplexing over radio-over-fiber (RoF) links, we report the experimental evaluation of an SCI detection scheme based on a time-domain correlation (TDC) technique in comparison with the conventional maximum likelihood (ML) approach. When compared with the ML method, it is shown that the TDC method improves detection performance over both 20 and 40 km of standard single mode fiber (SSMF) links. We also report a performance analysis of the TDC scheme in noisy visible light communication channel models after propagation through 40 km of SSMF. Experimental and simulation results confirm that the TDC method is attractive for practical orthogonal frequency division multiplexing-based RoF and fiber-wireless systems. Unlike the ML method, another key benefit of the TDC is that it requires no channel estimation.

The subcortical maternal complex controls symmetric division of mouse zygotes by regulating F-actin dynamics.

PubMed

Yu, Xing-Jiang; Yi, Zhaohong; Gao, Zheng; Qin, Dandan; Zhai, Yanhua; Chen, Xue; Ou-Yang, Yingchun; Wang, Zhen-Bo; Zheng, Ping; Zhu, Min-Sheng; Wang, Haibin; Sun, Qing-Yuan; Dean, Jurrien; Li, Lei

2014-09-11

Maternal effect genes play critical roles in early embryogenesis of model organisms where they have been intensively investigated. However, their molecular function in mammals remains largely unknown. Recently, we identified a subcortical maternal complex (SCMC) that contains four proteins encoded by maternal effect genes (Mater, Filia, Floped and Tle6). Here we report that TLE6, similar to FLOPED and MATER, stabilizes the SCMC and is necessary for cleavage beyond the two-cell stage of development. We document that the SCMC is required for formation of the cytoplasmic F-actin meshwork that controls the central position of the spindle and ensures symmetric division of mouse zygotes. We further demonstrate that the SCMC controls formation of the actin cytoskeleton specifically via Cofilin, a key regulator of F-actin assembly. Our results provide molecular insight into the physiological function of TLE6, its interaction with the SCMC and their roles in the symmetric division of the zygote in early mouse development.

Staff - Nina T. Harun | Alaska Division of Geological & Geophysical Surveys

Science.gov Websites

mapping of the Upper Jurassic Naknek Formation in a footwall syncline associated with the Bruin Bay fault Ivishak Formation in the northeastern Brooks Range, Alaska: University of Alaska Fairbanks, M.S. thesis Triassic Ivishak Formation in the Sadlerochit Mountains, northeastern Alaska: Alaska Division of Geological

Propulsion and Energetics Panel Working Group 14 on Suitable Averaging Techniques in Non-Uniform Internal Flows

DTIC Science & Technology

1983-06-01

PANEL WORKING GROUP 14 on SUITABLE AVERAGING TECHNIQUES IN NON-UNIFORM INTERNAL FLOWS Edited by M.Pianko Office National d’Etudes et de...d’Etudes et de Recherches Aerospatiales Pratt and Whitney Government Products Division Rocketdyne Division of Rockwell International , Inc. Teledyne CAE...actions exerted by individual components on the gas flow must be known. These specific component effects are distributed internally within the

An Analysis of the Marine Corps Individual Ready Reserve Screening Process

DTIC Science & Technology

2015-03-01

Identification of anticipated requirements among M&RA and HQMC’s Manpower Management division (MM) and Manpower Plans and Policy division (MP) for...Retain a militruy identification card • Attend musters when ordered • Maintain physical, height, and weight standards IRR MEMBER’S YEARS IN THE...their components.32 The identification of these specialties and required training might prove advantageous to the AC and RC.33 b. The Transition to

Determination de la Distribution Granulometrique des Particules par la Mesure de la Transmission d’un Aerosol Sedimentant (Aerosol Size Distribution Using the Extinction-Sedimentation Inversion Technique),

DTIC Science & Technology

1982-11-01

est la longueur du chemin optique. La fonction K(xn, n) peut Stre evaluee 1 l’aide de la theorie de Mie. Elle est du type oscillant et tend vers 2...Directeur, Division de l1informatlque - Directeur, Division de la Propulsion 10 - Bibliotheque - M. G. Roy ( auteur ) - Dr R. Lavertu - Dr B.T.N

Cognitive Code-Division Channelization

DTIC Science & Technology

2011-04-01

22] G. N. Karystinos and D. A. Pados, “New bounds on the total squared correlation and optimum design of DS - CDMA binary signature sets,” IEEE Trans...Commun., vol. 51, pp. 48-51, Jan. 2003. [23] C. Ding, M. Golin, and T. Klve, “Meeting the Welch and Karystinos- Pados bounds on DS - CDMA binary...receiver pair coexisting with a primary code-division multiple-access ( CDMA ) system. Our objective is to find the optimum transmitting power and code

Ergonomic Survey, Hill AFB, UT

DTIC Science & Technology

1991-03-01

Hygiene NIOSH a. CAPT Roger Jensen, Division of Safety Research b. LCDR Patricia Schnitzer, Division of Safety Research c. GS-14 Vern Putz-Anderson... Patricia Schnitzer was provided a current listing (October 1988-October 1990) of the CTD cases from the PHOENIX data base for review. There were a...MOTIVE AERO-MOTIVE U.K. LTD. RO. Box 2678 1090 Brevik Place Rassau Industrial Estate Kalamazoo, M1 49003 Mississauga, Ontario Ebbw Vale Gwent NP3 5SD Ph

Role Of Social Networks In Resilience Of Naval Recruits: A Quantitative Analysis

DTIC Science & Technology

2016-06-01

comprises 1,297 total surveys from a total of eight divisions of recruits at two different time periods. Quantitative analyses using surveys and network... surveys from a total of eight divisions of recruits at two different time periods. Quantitative analyses using surveys and network data examine the effects...NETWORKS IN RESILIENCE OF NAVAL RECRUITS: A QUANTITATIVE ANALYSIS by Andrea M. Watling June 2016 Thesis Advisor: Edward H. Powley Co

Dissecting a new connection between cytokinin and jasmonic acid in control of leaf growth

USDA-ARS?s Scientific Manuscript database

Plant growth is mediated by two cellular processes: division and elongation. The maize leaf is an excellent model to study plant growth since these processes are spatially separated into discreet zones - a division zone (DZ), transition zone (TZ), and elongation zone (EZ) - at the base of the leaf. ...

Strategic Planning in a Government Organization: The Experience of the Technical Information Division at NRaD.

ERIC Educational Resources Information Center

Cathcart, Margaret E.

1997-01-01

Describes development of strategic objectives at the Research, Development, Test, and Evaluation Division of the Naval Command, Control and Ocean Surveillance Center (NRaD). Discusses its plan to achieve these objectives. States that the organization's responsibilities span the continuum of information using technical communication skills. Uses…

ZapE Is a Novel Cell Division Protein Interacting with FtsZ and Modulating the Z-Ring Dynamics

PubMed Central

Marteyn, Benoit S.; Karimova, Gouzel; Fenton, Andrew K.; Gazi, Anastasia D.; West, Nicholas; Touqui, Lhousseine; Prevost, Marie-Christine; Betton, Jean-Michel; Poyraz, Oemer; Ladant, Daniel; Gerdes, Kenn; Sansonetti, Philippe J.; Tang, Christoph M.

2014-01-01

ABSTRACT Bacterial cell division requires the formation of a mature divisome complex positioned at the midcell. The localization of the divisome complex is determined by the correct positioning, assembly, and constriction of the FtsZ ring (Z-ring). Z-ring constriction control remains poorly understood and (to some extent) controversial, probably due to the fact that this phenomenon is transient and controlled by numerous factors. Here, we characterize ZapE, a novel ATPase found in Gram-negative bacteria, which is required for growth under conditions of low oxygen, while loss of zapE results in temperature-dependent elongation of cell shape. We found that ZapE is recruited to the Z-ring during late stages of the cell division process and correlates with constriction of the Z-ring. Overexpression or inactivation of zapE leads to elongation of Escherichia coli and affects the dynamics of the Z-ring during division. In vitro, ZapE destabilizes FtsZ polymers in an ATP-dependent manner. PMID:24595368

Industrial workers' health and environmental pollution under the new international division of labor: the Taiwan experience.

PubMed Central

Chen, M S; Huang, C L

1997-01-01

Using Taiwan as an example, this paper conducts a historical analysis of the relationship between economic development in the new international division of labor and environmental pollution and industrial workers' health. Three industries-asbestos, plastic, and dye-were chosen for case studies. We trace the emergence of each industry in Taiwan and study each industry's protection of workers' health and environmental quality. Under the new international division of labor, the state's prioritization of economic development leads to lenient regulation. Under such state policies, employers have few incentives to invest in the protection of their workers' health and in the control of environmental pollution. Workers and the public are constrained in their efforts to protect their own health and prevent environmental pollution. This situation is exemplified by the deplorable working conditions and inadequate environmental pollution controls in the asbestos, plastic, and dye industries. Workers' health and the public's health are greatly compromised by economic development in the new international division of labor. Images p1228-a p1228-b PMID:9240119

Division of Labor in Biofilms: the Ecology of Cell Differentiation.

PubMed

van Gestel, Jordi; Vlamakis, Hera; Kolter, Roberto

2015-04-01

The dense aggregation of cells on a surface, as seen in biofilms, inevitably results in both environmental and cellular heterogeneity. For example, nutrient gradients can trigger cells to differentiate into various phenotypic states. Not only do cells adapt physiologically to the local environmental conditions, but they also differentiate into cell types that interact with each other. This allows for task differentiation and, hence, the division of labor. In this article, we focus on cell differentiation and the division of labor in three bacterial species: Myxococcus xanthus, Bacillus subtilis, and Pseudomonas aeruginosa. During biofilm formation each of these species differentiates into distinct cell types, in some cases leading to cooperative interactions. The division of labor and the cooperative interactions between cell types are assumed to yield an emergent ecological benefit. Yet in most cases the ecological benefits have yet to be elucidated. A notable exception is M. xanthus, in which cell differentiation within fruiting bodies facilitates the dispersal of spores. We argue that the ecological benefits of the division of labor might best be understood when we consider the dynamic nature of both biofilm formation and degradation.

Crown-root morphology of lower incisors in patients with class III malocclusion.

PubMed

Wang, Bo; Shen, Guofang; Fang, Bing; Zhang, Li

2012-07-01

The purpose of this study was to investigate the crown-root morphology of lower incisors in patients with class III malocclusion using cone-beam computed tomography. Cone-beam computed tomography images were analyzed from 53 adult class I patients (group 1), 37 preadolescent class III patients (group 2), and 66 adult class III patients (group 3) comprising 3 divisions (divisions 1, 2, and 3 corresponded to mild, moderate, and severe class III malocclusions). The size and crown-root angulations of lower incisors in different groups and divisions were statistically appraised with group 1 used as the control group. No significant differences were found for the size of lower incisors among different groups and divisions (P > 0.05). Compared with group 1, the crown-root angulations of lower incisors in groups 2 and 3 were significantly larger (P < 0.01), and among 3 divisions, the lower incisors of division 3 rather than divisions 2 and 3 exhibited larger crown-root angulations (P < 0.01). Attention should be paid to the enlarged crown-root angulations of lower incisors in class III patients during orthodontic and orthognathic treatment, especially in severe ones.

Publications - Beikman, H.M., 1980 | Alaska Division of Geological &

Science.gov Websites

main content USGS Beikman, H.M., 1980 Publication Details Title: Geologic map of Alaska Authors Warehouse Bibliographic Reference Beikman, H.M., 1980, Geologic map of Alaska: U.S. Geological Survey, 1 USGS website Maps & Other Oversized Sheets Maps & Other Oversized Sheets Sheet 1 Geologic Map

Resveratrol protects mouse embryonic stem cells from ionizing radiation by accelerating recovery from DNA strand breakage.

PubMed

Denissova, Natalia G; Nasello, Cara M; Yeung, Percy L; Tischfield, Jay A; Brenneman, Mark A

2012-01-01

Resveratrol has elicited many provocative anticancer effects in laboratory animals and cultured cells, including reduced levels of oxidative DNA damage, inhibition of tumor initiation and progression and induction of apoptosis in tumor cells. Use of resveratrol as a cancer-preventive agent in humans will require that its anticancer effects not be accompanied by damage to normal tissue stem or progenitor cells. In mouse embryonic stem cells (mESC) or early mouse embryos exposed to ethanol, resveratrol has been shown to suppress apoptosis and promote survival. However, in cells exposed to genotoxic stress, survival may come at the expense of genome stability. To learn whether resveratrol can protect stem cells from DNA damage and to study its effects on genomic integrity, we exposed mESC pretreated with resveratrol to ionizing radiation (IR). Forty-eight hours pretreatment with a comparatively low concentration of resveratrol (10 μM) improved survival of mESC >2-fold after exposure to 5 Gy of X-rays. Cells pretreated with resveratrol sustained the same levels of reactive oxygen species and DNA strand breakage after IR as mock-treated controls, but repaired DNA damage more rapidly and resumed cell division sooner. Frequencies of IR-induced mutation at a chromosomal reporter locus were not increased in cells pretreated with resveratrol as compared with controls, indicating that resveratrol can improve viability in mESC after DNA damage without compromising genomic integrity.

Reversible Lipid Accumulation and Associated Division Arrest of Mycobacterium avium in Lipoprotein-Induced Foamy Macrophages May Resemble Key Events during Latency and Reactivation of Tuberculosis

PubMed Central

Caire-Brändli, Irène; Papadopoulos, Alexia; Malaga, Wladimir; Marais, David; Canaan, Stéphane; Thilo, Lutz

2014-01-01

During the dormant phase of tuberculosis, Mycobacterium tuberculosis persists in lung granulomas by residing in foamy macrophages (FM) that contain abundant lipid bodies (LB) in their cytoplasm, allowing bacilli to accumulate lipids as intracytoplasmic lipid inclusions (ILI). An experimental model of FM is presented where bone marrow-derived mouse macrophages are infected with M. avium and exposed to very-low-density lipoprotein (VLDL) as a lipid source. Quantitative analysis of detailed electron microscope observations showed the following results. (i) Macrophages became foamy, and mycobacteria formed ILI, for which host triacylglycerides, rather than cholesterol, was essential. (ii) Lipid transfer occurred via mycobacterium-induced fusion between LB and phagosomes. (iii) Mycobacteria showed a thinned cell wall and became elongated but did not divide. (iv) Upon removal of VLDL, LB and ILI declined within hours, and simultaneous resumption of mycobacterial division restored the number of mycobacteria to the same level as that found in untreated control macrophages. This showed that the presence of ILI resulted in a reversible block of division without causing a change in the mycobacterial replication rate. Fluctuation between ILI either partially or fully extending throughout the mycobacterial cytoplasm was suggestive of bacterial cell cycle events. We propose that VLDL-driven FM constitute a well-defined cellular system in which to study changed metabolic states of intracellular mycobacteria that may relate to persistence and reactivation of tuberculosis. PMID:24478064

Prediction of Strong Earthquake Ground Motion for the M=7.4 and M=7.2 1999, Turkey Earthquakes based upon Geological Structure Modeling and Local Earthquake Recordings

NASA Astrophysics Data System (ADS)

Gok, R.; Hutchings, L.

2004-05-01

We test a means to predict strong ground motion using the Mw=7.4 and Mw=7.2 1999 Izmit and Duzce, Turkey earthquakes. We generate 100 rupture scenarios for each earthquake, constrained by a prior knowledge, and use these to synthesize strong ground motion and make the prediction. Ground motion is synthesized with the representation relation using impulsive point source Green's functions and synthetic source models. We synthesize the earthquakes from DC to 25 Hz. We demonstrate how to incorporate this approach into standard probabilistic seismic hazard analyses (PSHA). The synthesis of earthquakes is based upon analysis of over 3,000 aftershocks recorded by several seismic networks. The analysis provides source parameters of the aftershocks; records available for use as empirical Green's functions; and a three-dimensional velocity structure from tomographic inversion. The velocity model is linked to a finite difference wave propagation code (E3D, Larsen 1998) to generate synthetic Green's functions (DC < f < 0.5 Hz). We performed the simultaneous inversion for hypocenter locations and three-dimensional P-wave velocity structure of the Marmara region using SIMULPS14 along with 2,500 events. We also obtained source moment and corner frequency and individual station attenuation parameter estimates for over 500 events by performing a simultaneous inversion to fit these parameters with a Brune source model. We used the results of the source inversion to deconvolve out a Brune model from small to moderate size earthquake (M<4.0) recordings to obtain empirical Green's functions for the higher frequency range of ground motion (0.5 < f < 25.0 Hz). Work performed under the auspices of the U.S. Department of Energy by Lawrence Livermore National Laboratory under Contract W-7405-ENG-48.

Translatome analysis at the egg-to-embryo transition in sea urchin

PubMed Central

Chassé, Héloïse; Aubert, Julie; Boulben, Sandrine; Le Corguillé, Gildas; Corre, Erwan; Cormier, Patrick

2018-01-01

Abstract Early embryogenesis relies on the translational regulation of maternally stored mRNAs. In sea urchin, fertilization triggers a dramatic rise in translation activity, necessary for the onset of cell division. Here, the full spectrum of the mRNAs translated upon fertilization was investigated by polysome profiling and sequencing. The translatome of the early sea urchin embryo gave a complete picture of the polysomal recruitment dynamics following fertilization. Our results indicate that only a subset of maternal mRNAs were selectively recruited onto polysomes, with over-represented functional categories in the translated set. The increase in translation upon fertilization depends on the formation of translation initiation complexes following mTOR pathway activation. Surprisingly, mTOR pathway inhibition differentially affected polysomal recruitment of the newly translated mRNAs, which thus appeared either mTOR-dependent or mTOR-independent. Therefore, our data argue for an alternative to the classical cap-dependent model of translation in early development. The identification of the mRNAs translated following fertilization helped assign translational activation events to specific mRNAs. This translatome is the first step to a comprehensive analysis of the molecular mechanisms governing translation upon fertilization and the translational regulatory networks that control the egg-to-embryo transition as well as the early steps of embryogenesis. PMID:29660001

Translatome analysis at the egg-to-embryo transition in sea urchin.

PubMed

Chassé, Héloïse; Aubert, Julie; Boulben, Sandrine; Le Corguillé, Gildas; Corre, Erwan; Cormier, Patrick; Morales, Julia

2018-05-18

Early embryogenesis relies on the translational regulation of maternally stored mRNAs. In sea urchin, fertilization triggers a dramatic rise in translation activity, necessary for the onset of cell division. Here, the full spectrum of the mRNAs translated upon fertilization was investigated by polysome profiling and sequencing. The translatome of the early sea urchin embryo gave a complete picture of the polysomal recruitment dynamics following fertilization. Our results indicate that only a subset of maternal mRNAs were selectively recruited onto polysomes, with over-represented functional categories in the translated set. The increase in translation upon fertilization depends on the formation of translation initiation complexes following mTOR pathway activation. Surprisingly, mTOR pathway inhibition differentially affected polysomal recruitment of the newly translated mRNAs, which thus appeared either mTOR-dependent or mTOR-independent. Therefore, our data argue for an alternative to the classical cap-dependent model of translation in early development. The identification of the mRNAs translated following fertilization helped assign translational activation events to specific mRNAs. This translatome is the first step to a comprehensive analysis of the molecular mechanisms governing translation upon fertilization and the translational regulatory networks that control the egg-to-embryo transition as well as the early steps of embryogenesis.

15. Amygdala pain mechanisms

PubMed Central

Neugebauer, Volker

2015-01-01

A limbic brain area the amygdala plays a key role in emotional responses and affective states and disorders such as learned fear, anxiety and depression. The amygdala has also emerged as an important brain center for the emotional-affective dimension of pain and for pain modulation. Hyperactivity in the laterocapsular division of the central nucleus of the amygdala (CeLC, also termed the “nociceptive amygdala”) accounts for pain-related emotional responses and anxiety-like behavior. Abnormally enhanced output from the CeLC is the consequence of an imbalance between excitatory and inhibitory mechanisms. Impaired inhibitory control mediated by a cluster of GABAergic interneurons in the intercalated cell masses (ITC) allows the development of glutamate- and neuropeptide-driven synaptic plasticity of excitatory inputs from the brainstem (parabrachial area) and from the lateral-basolateral amygdala network (LA-BLA, site of integration of polymodal sensory information). BLA hyperactivity also generates abnormally enhanced feedforward inhibition of principal cells in the medial prefrontal cortex (mPFC), a limbic cortical area that is strongly interconnected with the amygdala. Pain-related mPFC deactivation results in cognitive deficits and failure to engage cortically driven ITC-mediated inhibitory control of amygdala processing. Impaired cortical control allows the uncontrolled persistence of amygdala pain mechanisms. PMID:25846623

Cyclin B in mouse oocytes and embryos: importance for human reproduction and aneuploidy.

PubMed

Polański, Zbigniew; Homer, Hayden; Kubiak, Jacek Z

2012-01-01

Oocyte maturation and early embryo development require precise coordination between cell cycle progression and the developmental programme. Cyclin B plays a major role in this process: its accumulation and degradation is critical for driving the cell cycle through activation and inactivation of the major cell cycle kinase, CDK1. CDK1 activation is required for M-phase entry whereas its inactivation leads to exit from M-phase. The tempo of oocyte meiotic and embryonic mitotic divisions is set by the rate of cyclin B accumulation and the timing of its destruction. By controlling when cyclin B destruction is triggered and by co-ordinating this with the completion of chromosome alignment, the spindle assembly checkpoint (SAC) is a critical quality control system important for averting aneuploidy and for building in the flexibility required to better integrate cell cycle progression with development. In this review we focus on cyclin B metabolism in mouse oocytes and embryos and illustrate how the cell cycle-powered clock (in fact cyclin B-powered clock) controls oocyte maturation and early embryo development, thereby providing important insight into human reproduction and potential causes of Down syndrome.

The Impact of Mitochondrial Complex Inhibition on mESC Differentiation

EPA Science Inventory

The Impact of Mitochondrial Complex Inhibition on mESC Differentiation JE Royland, SH Warren, S Jeffay, MR Hoopes, HP Nichols, ES Hunter U.S. Environmental Protection Agency, Integrated Systems Toxicology Division, Research Triangle Park, NC The importance of mitochondrial funct...

Publications - PIR 2015-5-1 | Alaska Division of Geological & Geophysical

Science.gov Websites

content DGGS PIR 2015-5-1 Publication Details Title: An introduction to 2014 field studies in western Cook , M.A., 2015, An introduction to 2014 field studies in western Cook Inlet, Alaska, in Wartes, M.A., ed

Effective GTP-replacing FtsZ inhibitors and antibacterial mechanism of action.

PubMed

Artola, Marta; Ruiz-Avila, Laura B; Vergoñós, Albert; Huecas, Sonia; Araujo-Bazán, Lidia; Martín-Fontecha, Mar; Vázquez-Villa, Henar; Turrado, Carlos; Ramírez-Aportela, Erney; Hoegl, Annabelle; Nodwell, Matthew; Barasoain, Isabel; Chacón, Pablo; Sieber, Stephan A; Andreu, Jose M; López-Rodríguez, María L

2015-03-20

Essential cell division protein FtsZ is considered an attractive target in the search for antibacterials with novel mechanisms of action to overcome the resistance problem. FtsZ undergoes GTP-dependent assembly at midcell to form the Z-ring, a dynamic structure that evolves until final constriction of the cell. Therefore, molecules able to inhibit its activity will eventually disrupt bacterial viability. In this work, we report a new series of small molecules able to replace GTP and to specifically inhibit FtsZ, blocking the bacterial division process. These new synthesized inhibitors interact with the GTP-binding site of FtsZ (Kd = 0.4-0.8 μM), display antibacterial activity against Gram-positive pathogenic bacteria, and show selectivity against tubulin. Biphenyl derivative 28 stands out as a potent FtsZ inhibitor (Kd = 0.5 μM) with high antibacterial activity [MIC (MRSA) = 7 μM]. In-depth analysis of the mechanism of action of compounds 22, 28, 33, and 36 has revealed that they act as effective inhibitors of correct FtsZ assembly, blocking bacterial division and thus leading to filamentous undivided cells. These findings provide a compelling rationale for the development of compounds targeting the GTP-binding site as antibacterial agents and open the door to antibiotics with novel mechanisms of action.

Cell size control and homeostasis in bacteria

NASA Astrophysics Data System (ADS)

Bradde, Serena; Taheri, Sattar; Sauls, John; Hill, Nobert; Levine, Petra; Paulsson, Johan; Vergassola, Massimo; Jun, Suckjoon

2015-03-01

How cells control their size is a fundamental question in biology. The mechanisms for sensing size, time, or a combination of the two are not supported by experimental evidence. By analysing distributions of size at division at birth and generation time of hundreds of thousands of Gram-negative E. coli and Gram-positive B. subtilis cells under a wide range of tightly controlled steady-state growth conditions, we are now in the position to validate different theoretical models. In this talk I will present all possible models in details and present a general mechanism that quantitatively explains all measurable aspects of growth and cell division at both population and single-cell levels.

Scalable autonomous operations of unmanned assets

NASA Astrophysics Data System (ADS)

Jung, Sunghun

Although there have been great theoretical advances in the region of Unmanned Aerial Vehicle (UAV) autonomy, applications of those theories into real world are still hesitated due to unexpected disturbances. Most of UAVs which are currently used are mainly, strictly speaking, Remotely Piloted Vehicles (RPA) since most works related with the flight control, sensor data analysis, and decision makings are done by human operators. To increase the degree of autonomy, many researches are focused on developing Unmanned Autonomous Aerial Vehicle (UAAV) which can takeoff, fly to the interested area by avoiding unexpected obstacles, perform various missions with decision makings, come back to the base station, and land on by itself without any human operators. To improve the performance of UAVs, the accuracies of position and orientation sensors are enhanced by integrating a Unmanned Ground Vehicle (UGV) or a solar compass to a UAV; Position sensor accuracy of a GPS sensor on a UAV is improved by referencing the position of a UGV which is calculated by using three GPS sensors and Weighted Centroid Localization (WCL) method; Orientation sensor accuracy is improved as well by using Three Pixel Theorem (TPT) and integrating a solar compass which composed of nine light sensors to a magnetic compass. Also, improved health management of a UAV is fulfilled by developing a wireless autonomous charging station which uses four pairs of transmitter and receiver magnetic loops with four robotic arms. For the software aspect, I also analyze the error propagation of the proposed mission planning hierarchy to achieve the safest size of the buffer zone. In addition, among seven future research areas regarding UAV, this paper mainly focuses on developing algorithms of path planning, trajectory generation, and cooperative tactics for the operations of multiple UAVs using GA based multiple Traveling Salesman Problem (mTSP) which is solved by dividing into m number of Traveling Salesman Problems (TSP) using two region division methods such as Uniform Region Division (URD) and K-means Voronoi Region Division (KVRD). The topic of the maximum fuel efficiency is also dealt to ensure the minimum amount fuel consumption to perform surveillance on a given region using multiple UAVs. Last but not least, I present an application example of cattle roundup with two UAVs and two animals using the feedback linearization controller.

Novel division level bacterial diversity in a Yellowstone hot spring.

PubMed

Hugenholtz, P; Pitulle, C; Hershberger, K L; Pace, N R

1998-01-01

A culture-independent molecular phylogenetic survey was carried out for the bacterial community in Obsidian Pool (OP), a Yellowstone National Park hot spring previously shown to contain remarkable archaeal diversity (S. M. Barns, R. E. Fundyga, M. W. Jeffries, and N. R. Page, Proc. Natl. Acad. Sci. USA 91:1609-1613, 1994). Small-subunit rRNA genes (rDNA) were amplified directly from OP sediment DNA by PCR with universally conserved or Bacteria-specific rDNA primers and cloned. Unique rDNA types among > 300 clones were identified by restriction fragment length polymorphism, and 122 representative rDNA sequences were determined. These were found to represent 54 distinct bacterial sequence types or clusters (> or = 98% identity) of sequences. A majority (70%) of the sequence types were affiliated with 14 previously recognized bacterial divisions (main phyla; kingdoms); 30% were unaffiliated with recognized bacterial divisions. The unaffiliated sequence types (represented by 38 sequences) nominally comprise 12 novel, division level lineages termed candidate divisions. Several OP sequences were nearly identical to those of cultivated chemolithotrophic thermophiles, including the hydrogen-oxidizing Calderobacterium and the sulfate reducers Thermodesulfovibrio and Thermodesulfobacterium, or belonged to monophyletic assemblages recognized for a particular type of metabolism, such as the hydrogen-oxidizing Aquificales and the sulfate-reducing delta-Proteobacteria. The occurrence of such organisms is consistent with the chemical composition of OP (high in reduced iron and sulfur) and suggests a lithotrophic base for primary productivity in this hot spring, through hydrogen oxidation and sulfate reduction. Unexpectedly, no archaeal sequences were encountered in OP clone libraries made with universal primers. Hybridization analysis of amplified OP DNA with domain-specific probes confirmed that the analyzed community rDNA from OP sediment was predominantly bacterial. These results expand substantially our knowledge of the extent of bacterial diversity and call into question the commonly held notion that Archaea dominate hydrothermal environments. Finally, the currently known extent of division level bacterial phylogenetic diversity is collated and summarized.

Myc/Mycn-mediated glycolysis enhances mouse spermatogonial stem cell self-renewal

PubMed Central

Kanatsu-Shinohara, Mito; Tanaka, Takashi; Ogonuki, Narumi; Ogura, Atsuo; Morimoto, Hiroko; Cheng, Pei Feng; Eisenman, Robert N.; Trumpp, Andreas; Shinohara, Takashi

2016-01-01

Myc plays critical roles in the self-renewal division of various stem cell types. In spermatogonial stem cells (SSCs), Myc controls SSC fate decisions because Myc overexpression induces enhanced self-renewal division, while depletion of Max, a Myc-binding partner, leads to meiotic induction. However, the mechanism by which Myc acts on SSC fate is unclear. Here we demonstrate a critical link between Myc/Mycn gene activity and glycolysis in SSC self-renewal. In SSCs, Myc/Mycn are regulated by Foxo1, whose deficiency impairs SSC self-renewal. Myc/Mycn-deficient SSCs not only undergo limited self-renewal division but also display diminished glycolytic activity. While inhibition of glycolysis decreased SSC activity, chemical stimulation of glycolysis or transfection of active Akt1 or Pdpk1 (phosphoinositide-dependent protein kinase 1 ) augmented self-renewal division, and long-term SSC cultures were derived from a nonpermissive strain that showed limited self-renewal division. These results suggested that Myc-mediated glycolysis is an important factor that increases the frequency of SSC self-renewal division. PMID:28007786

Myc/Mycn-mediated glycolysis enhances mouse spermatogonial stem cell self-renewal.

PubMed

Kanatsu-Shinohara, Mito; Tanaka, Takashi; Ogonuki, Narumi; Ogura, Atsuo; Morimoto, Hiroko; Cheng, Pei Feng; Eisenman, Robert N; Trumpp, Andreas; Shinohara, Takashi

2016-12-01

Myc plays critical roles in the self-renewal division of various stem cell types. In spermatogonial stem cells (SSCs), Myc controls SSC fate decisions because Myc overexpression induces enhanced self-renewal division, while depletion of Max, a Myc-binding partner, leads to meiotic induction. However, the mechanism by which Myc acts on SSC fate is unclear. Here we demonstrate a critical link between Myc/Mycn gene activity and glycolysis in SSC self-renewal. In SSCs, Myc/Mycn are regulated by Foxo1, whose deficiency impairs SSC self-renewal. Myc/Mycn-deficient SSCs not only undergo limited self-renewal division but also display diminished glycolytic activity. While inhibition of glycolysis decreased SSC activity, chemical stimulation of glycolysis or transfection of active Akt1 or Pdpk1 (phosphoinositide-dependent protein kinase 1 ) augmented self-renewal division, and long-term SSC cultures were derived from a nonpermissive strain that showed limited self-renewal division. These results suggested that Myc-mediated glycolysis is an important factor that increases the frequency of SSC self-renewal division. © 2016 Kanatsu-Shinohara et al.; Published by Cold Spring Harbor Laboratory Press.

Mammalian aPKC/Par polarity complex mediated regulation of epithelial division orientation and cell fate

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vorhagen, Susanne; Niessen, Carien M., E-mail: carien.niessen@uni-koeln.de

2014-11-01

Oriented cell division is a key regulator of tissue architecture and crucial for morphogenesis and homeostasis. Balanced regulation of proliferation and differentiation is an essential property of tissues not only to drive morphogenesis but also to maintain and restore homeostasis. In many tissues orientation of cell division is coupled to the regulation of differentiation producing daughters with similar (symmetric cell division, SCD) or differential fate (asymmetric cell division, ACD). This allows the organism to generate cell lineage diversity from a small pool of stem and progenitor cells. Division orientation and/or the ratio of ACD/SCD need to be tightly controlled. Lossmore » of orientation or an altered ratio can promote overgrowth, alter tissue architecture and induce aberrant differentiation, and have been linked to morphogenetic diseases, cancer and aging. A key requirement for oriented division is the presence of a polarity axis, which can be established through cell intrinsic and/or extrinsic signals. Polarity proteins translate such internal and external cues to drive polarization. In this review we will focus on the role of the polarity complex aPKC/Par3/Par6 in the regulation of division orientation and cell fate in different mammalian epithelia. We will compare the conserved function of this complex in mitotic spindle orientation and distribution of cell fate determinants and highlight common and differential mechanisms in which this complex is used by tissues to adapt division orientation and cell fate to the specific properties of the epithelium.« less

On-the-Job Training and Social Learning Theory. A Literature Review

DTIC Science & Technology

1980-05-01

Trott , H. An Analysis of a Human Relations Training PoA? m for Managers and Supervisors, PhD dissertation, University of Maryland, 1976. 153 53 I;,, 1...Division of HumRRO in Alexandria, Virginia, where Dr. Robert J. Seidel is the Division rirector. Mr. Paul E. Loustaunau was the Project Director. The...ways: a training method based on social learning theory has been used to teach managers to deal more effectively with human relations problems

Development of the Zebra Danio Model: Carcinogenesis and Gene Transfer Studies

DTIC Science & Technology

1996-09-01

J., and Enomoto, M. (1988). Liver cell carcinomas in the medaka (Oryzias latipes) induced by methylazoxymethanol-acetate. J. Comp. Path. 98, 441-452...accelerate steroid- induced cell division in Xenopus oocytes (Sadler et al., 1986). More recently, ras p21 has been implicated in the transduction of a... induced cell division in Xenopus laevis oocytes. Mol Cell Biol 6:719-722. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989). Molecular Cloning: A

Evaluation of Evidence for Altered Behavior and Auditory Deficits in Fishes Due to Human-Generated Noise Sources

DTIC Science & Technology

2006-04-01

prepared by the Research and Animal Care Branch, Code 2351, of the Biosciences Division, Code 235, SSC San Diego. This is a work of the United...and Animal Care Branch Under authority of M. Rothe, Head Biosciences Division i EXECUTIVE SUMMARY In this study, we have evaluated peer... sharks , skates, and rays) and teleost fishes (modern bony fishes) and provide recommendations for research to address remaining issues. Clear responses

Business Opportunities Guide

DTIC Science & Technology

1987-01-01

Electronic Systems Division Business Opportunities Guide. We remain committed to providing industry the best information possible on pending ESD...Commander Justific ill,):, By_ D.is’ * . ’ . Dist, : :,. ELECTRONIC SYSTEMS DIVISION BUSINESS OPPORTUNITIES GUIDE The complex Command, Control...Communications (C) systems necessary for tomorrow’s Air Foice can only be developed by a stronq’ESD/industry team. Our first Business Opportunities Guide

75 FR 60490 - 60-Day Notice of Proposed Information Collection: Bureau of Educational and Cultural Affairs...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-30

... Policy and Evaluation, Evaluation Division Survey Question Bank. OMB Control Number: 1405-0158. Type of... Cultural Affairs, Office of Policy and Evaluation, Evaluation Division (ECA/P/ V). Form Number: New surveys...-ROM submissions): ECA/P/V, SA-5, 5th Floor, Department of State, Washington, DC 20522-0505. Fax: 202...

Sporulation-specific cell division defects in ylmE mutants of Streptomyces coelicolor are rescued by additional deletion of ylmD.

PubMed

Zhang, Le; Willemse, Joost; Hoskisson, Paul A; van Wezel, Gilles P

2018-05-09

Cell division during the reproductive phase of the Streptomyces life-cycle requires tight coordination between synchronous formation of multiple septa and DNA segregation. One remarkable difference with most other bacterial systems is that cell division in Streptomyces is positively controlled by the recruitment of FtsZ by SsgB. Here we show that deletion of ylmD (SCO2081) or ylmE (SCO2080), which lie in operon with ftsZ in the dcw cluster of actinomycetes, has major consequences for sporulation-specific cell division in Streptomyces coelicolor. Electron and fluorescence microscopy demonstrated that ylmE mutants have a highly aberrant phenotype with defective septum synthesis, and produce very few spores with low viability and high heat sensitivity. FtsZ-ring formation was also highly disturbed in ylmE mutants. Deletion of ylmD had a far less severe effect on sporulation. Interestingly, the additional deletion of ylmD restored sporulation to the ylmE null mutant. YlmD and YlmE are not part of the divisome, but instead localize diffusely in aerial hyphae, with differential intensity throughout the sporogenic part of the hyphae. Taken together, our work reveals a function for YlmD and YlmE in the control of sporulation-specific cell division in S. coelicolor, whereby the presence of YlmD alone results in major developmental defects.

Stable Regulation of Cell Cycle Events in Mycobacteria: Insights From Inherently Heterogeneous Bacterial Populations.

PubMed

Logsdon, Michelle M; Aldridge, Bree B

2018-01-01

Model bacteria, such as E. coli and B. subtilis , tightly regulate cell cycle progression to achieve consistent cell size distributions and replication dynamics. Many of the hallmark features of these model bacteria, including lateral cell wall elongation and symmetric growth and division, do not occur in mycobacteria. Instead, mycobacterial growth is characterized by asymmetric polar growth and division. This innate asymmetry creates unequal birth sizes and growth rates for daughter cells with each division, generating a phenotypically heterogeneous population. Although the asymmetric growth patterns of mycobacteria lead to a larger variation in birth size than typically seen in model bacterial populations, the cell size distribution is stable over time. Here, we review the cellular mechanisms of growth, division, and cell cycle progression in mycobacteria in the face of asymmetry and inherent heterogeneity. These processes coalesce to control cell size. Although Mycobacterium smegmatis and Mycobacterium bovis Bacillus Calmette-Guérin (BCG) utilize a novel model of cell size control, they are similar to previously studied bacteria in that initiation of DNA replication is a key checkpoint for cell division. We compare the regulation of DNA replication initiation and strategies used for cell size homeostasis in mycobacteria and model bacteria. Finally, we review the importance of cellular organization and chromosome segregation relating to the physiology of mycobacteria and consider how new frameworks could be applied across the wide spectrum of bacterial diversity.

Gain control through divisive inhibition prevents abrupt transition to chaos in a neural mass model.

PubMed

Papasavvas, Christoforos A; Wang, Yujiang; Trevelyan, Andrew J; Kaiser, Marcus

2015-09-01

Experimental results suggest that there are two distinct mechanisms of inhibition in cortical neuronal networks: subtractive and divisive inhibition. They modulate the input-output function of their target neurons either by increasing the input that is needed to reach maximum output or by reducing the gain and the value of maximum output itself, respectively. However, the role of these mechanisms on the dynamics of the network is poorly understood. We introduce a novel population model and numerically investigate the influence of divisive inhibition on network dynamics. Specifically, we focus on the transitions from a state of regular oscillations to a state of chaotic dynamics via period-doubling bifurcations. The model with divisive inhibition exhibits a universal transition rate to chaos (Feigenbaum behavior). In contrast, in an equivalent model without divisive inhibition, transition rates to chaos are not bounded by the universal constant (non-Feigenbaum behavior). This non-Feigenbaum behavior, when only subtractive inhibition is present, is linked to the interaction of bifurcation curves in the parameter space. Indeed, searching the parameter space showed that such interactions are impossible when divisive inhibition is included. Therefore, divisive inhibition prevents non-Feigenbaum behavior and, consequently, any abrupt transition to chaos. The results suggest that the divisive inhibition in neuronal networks could play a crucial role in keeping the states of order and chaos well separated and in preventing the onset of pathological neural dynamics.

Subsurface microbial diversity in deep-granitic-fracture water in Colorado

USGS Publications Warehouse

Sahl, J.W.; Schmidt, R.; Swanner, E.D.; Mandernack, K.W.; Templeton, A.S.; Kieft, Thomas L.; Smith, R.L.; Sanford, W.E.; Callaghan, R.L.; Mitton, J.B.; Spear, J.R.

2008-01-01

A microbial community analysis using 16S rRNA gene sequencing was performed on borehole water and a granite rock core from Henderson Mine, a >1,000-meter-deep molybdenum mine near Empire, CO. Chemical analysis of borehole water at two separate depths (1,044 m and 1,004 m below the mine entrance) suggests that a sharp chemical gradient exists, likely from the mixing of two distinct subsurface fluids, one metal rich and one relatively dilute; this has created unique niches for microorganisms. The microbial community analyzed from filtered, oxic borehole water indicated an abundance of sequences from iron-oxidizing bacteria (Gallionella spp.) and was compared to the community from the same borehole after 2 weeks of being plugged with an expandable packer. Statistical analyses with UniFrac revealed a significant shift in community structure following the addition of the packer. Phospholipid fatty acid (PLFA) analysis suggested that Nitrosomonadales dominated the oxic borehole, while PLFAs indicative of anaerobic bacteria were most abundant in the samples from the plugged borehole. Microbial sequences were represented primarily by Firmicutes, Proteobacteria, and a lineage of sequences which did not group with any identified bacterial division; phylogenetic analyses confirmed the presence of a novel candidate division. This "Henderson candidate division" dominated the clone libraries from the dilute anoxic fluids. Sequences obtained from the granitic rock core (1,740 m below the surface) were represented by the divisions Proteobacteria (primarily the family Ralstoniaceae) and Firmicutes. Sequences grouping within Ralstoniaceae were also found in the clone libraries from metal-rich fluids yet were absent in more dilute fluids. Lineage-specific comparisons, combined with phylogenetic statistical analyses, show that geochemical variance has an important effect on microbial community structure in deep, subsurface systems. Copyright ?? 2008, American Society for Microbiology. All Rights Reserved.

Publications - RDF 2002-1 | Alaska Division of Geological & Geophysical

Science.gov Websites

., Clautice, K.H., Flynn, R.L., Grady, J.C., Munly, W.C., and Johnson, M.R. Publication Date: Aug 2002 ., Flynn, R.L., Grady, J.C., Munly, W.C., and Johnson, M.R., 2002, Major-oxide, minor-oxide, trace-element

76 FR 20375 - Meetings of Humanities Panel

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-12

... Committee Management Officer, National Endowment for the Humanities, Washington, DC 20506; telephone (202..., 2011. Time: 9 a.m. to 5 p.m. Location: Room 315. Program: This meeting will review applications for Landmark Workshops for School Teachers and Community College Faculty, submitted to the Division of...

Radiosensitivity of Mammalian Cells

PubMed Central

Walters, R. A.; Petersen, D. F.

1968-01-01

Radiation effects on macromolecular synthesis essential for the Chinese hamster cell to traverse the life cycle and to divide have been investigated. Life-cycle analysis techniques employing inhibitors of macromolecular synthesis were used in determining the kinetics of cell growth for specific segments of the population following spontaneous recovery from radiation-induced division delay. The results indicated that recovery does not occur in the absence of functional protein synthesis. Under conditions which inhibit normal RNA and DNA synthesis, irradiated cells can recover the capacity to traverse the life cycle and to divide. The stability of mRNA species coding for proteins essential for division in irradiated cells was also measured. The mean functional lifetime of these mRNA species was 1 hr. The data demonstrate the existence of a specific segment of the population consisting of cells which have completed transcription related to division but not concomitant translation and which can recover from the radiation injury without synthesis of additional RNA. Thus, initial recovery of the ability to divide has an obligate requirement for protein synthesis but no corresponding requirement for nucleic acid synthesis during the period when original messenger remains intact. PMID:5753224

Department of Defense August 2003 Influencer Poll 1 Overview Report

DTIC Science & Technology

2004-03-01

more positive change in 4 Ajzen , I . & Fishbein , M . (1980). Understanding Attitudes and Predicting...Social Behavior. Englewood Cliffs, NJ: Prentice-Hall. 5 Fishbein , M . & Ajzen , I . (1975). Belief, Attitude, Intention and Behavior: An Introduction to...Mavor, editors. Division of Behavioral and Social Sciences and Education. Washington, DC: The National Academies Press. 12 Fishbein , M ., Ajzen , I

THE ARCHITECTURE OF THE CASSINI DIVISION

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hedman, M. M.; Nicholson, P. D.; Baines, K. H.

2010-01-15

The Cassini Division in Saturn's rings contains a series of eight named gaps, three of which contain dense ringlets. Observations of stellar occultations by the Visual and Infrared Mapping Spectrometer onboard the Cassini spacecraft have yielded {approx}40 accurate and precise measurements of the radial position of the edges of all of these gaps and ringlets. These data reveal suggestive patterns in the shapes of many of the gap edges: the outer edges of the five gaps without ringlets are circular to within 1 km, while the inner edges of six of the gaps are eccentric, with apsidal precession rates consistentmore » with those expected for eccentric orbits near each edge. Intriguingly, the pattern speeds of these eccentric inner gap edges, together with that of the eccentric Huygens Ringlet, form a series with a characteristic spacing of 0.{sup 0}06 day{sup -1}. The two gaps with non-eccentric inner edges lie near first-order inner Lindblad resonances (ILRs) with moons. One such edge is close to the 5:4 ILR with Prometheus, and the radial excursions of this edge do appear to have an m = 5 component aligned with that moon. The other resonantly confined edge is the outer edge of the B ring, which lies near the 2:1 Mimas ILR. Detailed investigation of the B-ring-edge data confirm the presence of an m = 2 perturbation on the B-ring edge, but also show that during the course of the Cassini Mission, this pattern has drifted backward relative to Mimas. Comparisons with earlier occultation measurements going back to Voyager suggest the possibility that the m = 2 pattern is actually librating relative to Mimas with a libration frequency L {approx} 0.{sup 0}06 day{sup -1} (or possibly 0.{sup 0}12 day{sup -1}). In addition to the m = 2 pattern, the B-ring edge also has an m = 1 component that rotates around the planet at a rate close to the expected apsidal precession rate. Thus, the pattern speeds of the eccentric edges in the Cassini Division can be generated from various combinations of the pattern speeds of structures observed on the edge of the B ring. We therefore suggest that most of the gaps in the Cassini Division are produced by resonances involving perturbations from the massive edge of the B ring. We find that a combination of gravitational perturbations generated by the radial excursions in the B-ring edge and the gravitational perturbations from the Mimas 2:1 ILR yields terms in the equations of motion that should act to constrain the pericenter location of particle orbits in the vicinity of each of the eccentric inner gap edges in the Cassini Division. This alignment of pericenters could be responsible for forming the Cassini-Division Gaps and thus explain why these gaps are located where they are.« less

The architecture of the Cassini division

USGS Publications Warehouse

Hedman, M.M.; Nicholson, P.D.; Baines, K.H.; Buratti, B.J.; Sotin, Christophe; Clark, R.N.; Brown, R.H.; French, R.G.; Marouf, E.A.

2010-01-01

The Cassini Division in Saturn's rings contains a series of eight named gaps, three of which contain dense ringlets. Observations of stellar occultations by the Visual and Infrared Mapping Spectrometer onboard the Cassini spacecraft have yielded 40 accurate and precise measurements of the radial position of the edges of all of these gaps and ringlets. These data reveal suggestive patterns in the shapes of many of the gap edges: the outer edges of the five gaps without ringlets are circular to within 1 km, while the inner edges of six of the gaps are eccentric, with apsidal precession rates consistent with those expected for eccentric orbits near each edge. Intriguingly, the pattern speeds of these eccentric inner gap edges, together with that of the eccentric Huygens Ringlet, form a series with a characteristic spacing of 006 day-1. The two gaps with non-eccentric inner edges lie near first-order inner Lindblad resonances (ILRs) with moons. One such edge is close to the 5:4 ILR with Prometheus, and the radial excursions of this edge do appear to have an m = 5 component aligned with that moon. The other resonantly confined edge is the outer edge of the B ring, which lies near the 2:1 Mimas ILR. Detailed investigation of the B-ring-edge data confirm the presence of an m = 2 perturbation on the B-ring edge, but also show that during the course of the Cassini Mission, this pattern has drifted backward relative to Mimas. Comparisons with earlier occultation measurements going back to Voyager suggest the possibility that the m = 2 pattern is actually librating relative to Mimas with a libration frequency L 006 day-1 (or possibly 012 day -1). In addition to the m = 2 pattern, the B-ring edge also has an m = 1 component that rotates around the planet at a rate close to the expected apsidal precession rate (?? ?? ?? B ??? 5.??06 day -1). Thus, the pattern speeds of the eccentric edges in the Cassini Division can be generated from various combinations of the pattern speeds of structures observed on the edge of the B ring: ??p = ?? ?? ??B -jL for j = 1, 2, 3, ???, 7. We therefore suggest that most of the gaps in the Cassini Division are produced by resonances involving perturbations from the massive edge of the B ring. We find that a combination of gravitational perturbations generated by the radial excursions in the B-ring edge and the gravitational perturbations from the Mimas 2:1 ILR yields terms in the equations of motion that should act to constrain the pericenter location of particle orbits in the vicinity of each of the eccentric inner gap edges in the Cassini Division. This alignment of pericenters could be responsible for forming the Cassini-Division Gaps and thus explain why these gaps are located where they are. ?? 2010. The American Astronomical Society. All rights reserved.

Mammograms

MedlinePlus

... gov/widgets/fahc.html" width="243" height="179" title="Find a Health Center Widget" ... M.D., Community Oncology and Prevention Trials Research Group, Division of Cancer Prevention, National Cancer Institute, ...

Towards a mulitphase equation of state of Carbon from first principles

NASA Astrophysics Data System (ADS)

Correa, Alfredo; Benedict, Lorin; Schwegler, Eric

2007-03-01

Ab initio molecular dynamics and electronic structure calculation had become one of the most useful tools to investigate properties of materials. Unfortunately these atomistic detailed results are rarely reused in calculations at a higher level of description, such as fluid dynamics and finite elements calculations. In this talk we present a concrete example showing the way that first principles results can be expressed in a way that is useful for hydrodynamics calculations, in particular we show how to build a analytic equation of state for Carbon that involves solid (diamond and BC8) and liquid phases. Applications of this newly obtained equation of state will be presented. This work was performed under the auspices of the U.S. Dept. of Energy at the University of California/Lawrence Livermore National Laboratory under contract no. W-7405-Eng-48.

Damage evolution in viscoelastic polymers

NASA Astrophysics Data System (ADS)

Clements, B. E.

2000-04-01

Constitutive relations are derived for viscoelastic polymers. These relations are applicable to polymers for temperatures above their glass transition temperature and strain rates ranging from quasistatic up to shock regimes. Linear viscoelasticity is assumed for small tensile deformations but nonlinear effects, arising from void growth, become important at larger strains. Our void growth model is based on a generalization of Eshelby's Green's function solution to the problem of an ellipsoidal void in an elastic material. We apply our analysis to study the mechanical properties of polyvinyl acetate under dynamic loading conditions. Void concentration and aspect ratio considerations are found to be important in general deformation events. Uniaxial tension tends to favor aspect ratio change, while non-spherical voids are observed to evolve into spherical ones as tensile strain approaches triaxiality. [Research supported by the USDOE under contract W-7405-ENG-36

Individuality and universality in the growth-division laws of single E. coli cells

NASA Astrophysics Data System (ADS)

Kennard, Andrew S.; Osella, Matteo; Javer, Avelino; Grilli, Jacopo; Nghe, Philippe; Tans, Sander J.; Cicuta, Pietro; Cosentino Lagomarsino, Marco

2016-01-01

The mean size of exponentially dividing Escherichia coli cells in different nutrient conditions is known to depend on the mean growth rate only. However, the joint fluctuations relating cell size, doubling time, and individual growth rate are only starting to be characterized. Recent studies in bacteria reported a universal trend where the spread in both size and doubling times is a linear function of the population means of these variables. Here we combine experiments and theory and use scaling concepts to elucidate the constraints posed by the second observation on the division control mechanism and on the joint fluctuations of sizes and doubling times. We found that scaling relations based on the means collapse both size and doubling-time distributions across different conditions and explain how the shape of their joint fluctuations deviates from the means. Our data on these joint fluctuations highlight the importance of cell individuality: Single cells do not follow the dependence observed for the means between size and either growth rate or inverse doubling time. Our calculations show that these results emerge from a broad class of division control mechanisms requiring a certain scaling form of the "division hazard rate function," which defines the probability rate of dividing as a function of measurable parameters. This "model free" approach gives a rationale for the universal body-size distributions observed in microbial ecosystems across many microbial species, presumably dividing with multiple mechanisms. Additionally, our experiments show a crossover between fast and slow growth in the relation between individual-cell growth rate and division time, which can be understood in terms of different regimes of genome replication control.

Cell division and endoreduplication: doubtful engines of vegetative growth.

PubMed

John, Peter C L; Qi, Ruhu

2008-03-01

Currently, there is little information to indicate whether plant cell division and development is the collective effect of individual cell programming (cell-based) or is determined by organ-wide growth (organismal). Modulation of cell division does not confirm cell autonomous programming of cell expansion; instead, final cell size seems to be determined by the balance between cells formed and subsequent tissue growth. Control of growth in regions of the plant therefore has great importance in determining cell, organ and plant development. Here, we question the view that formation of new cells and their programmed expansion is the driving force of growth. We believe there is evidence that division does not drive, but requires, cell growth and a similar requirement for growth is detected in the modified cycle termed endoreduplication.

CoMIC, the hidden dynamics of mitochondrial inner compartments

PubMed Central

Cho, Bongki; Sun, Woong

2017-01-01

Mitochondria have evolutionarily, functionally and structurally distinct outer- (OMM) and inner-membranes (IMM). Thus, mitochondrial morphology is controlled by independent but coordinated activity of fission and fusion of the OMM and IMM. Constriction and division of the OMM are mediated by endocytosis-like machineries, which include dynamin-related protein 1 with additional cytosolic vesicle scissoring machineries such as actin filament and Dynamin 2. However, structural alteration of the IMM during mitochondrial division has been poorly understood. Recently, we found that the IMM and the inner compartments undergo transient and reversible constriction prior to the OMM division, which we termed CoMIC, Constriction of Mitochondrial Inner Compartment. In this short review, we further discuss the evolutionary perspective and the regulatory mechanism of CoMIC during mitochondrial division. PMID:28803609

CoMIC, the hidden dynamics of mitochondrial inner compartments.

PubMed

Cho, Bongki; Sun, Woong

2017-12-01

Mitochondria have evolutionarily, functionally and structurally distinct outer- (OMM) and inner-membranes (IMM). Thus, mitochondrial morphology is controlled by independent but coordinated activity of fission and fusion of the OMM and IMM. Constriction and division of the OMM are mediated by endocytosis-like machineries, which include dynamin-related protein 1 with additional cytosolic vesicle scissoring machineries such as actin filament and Dynamin 2. However, structural alteration of the IMM during mitochondrial division has been poorly understood. Recently, we found that the IMM and the inner compartments undergo transient and reversible constriction prior to the OMM division, which we termed CoMIC, Constriction of Mitochondrial Inner Compartment. In this short review, we further discuss the evolutionary perspective and the regulatory mechanism of CoMIC during mitochondrial division. [BMB Reports 2017; 50(12): 597-598].

Seismology program; California Division of Mines and Geology

USGS Publications Warehouse

Sherburne, R. W.

1981-01-01

The year 1980 marked the centennial of the California Division of Mines and Geology (CDMG) and a decade of the Division's involvement in seismology. Factors which contributed to the formation of a Seismology Group within CDMG included increased concerns for environmental and earthquake safety, interest in earthquake prediction, the 1971 San Fernando earthquake and the 1973 publication by CDMG of an urban geology master plan for California. Reasons to be concerned about California's earthquake problem are demonstrated by the accompanying table and the figures. Recent seismicity in California, the Southern California uplift reflecting changes in crustal strain, and other possible earthquake precursors have heightened concern among scientific and governmental groups about the possible occurrence of a major damaging earthquake )M>7) in California. 

Measuring Clinical Productivity.

PubMed

Hudson, Mark E; Lebovitz, Evan E

2018-06-01

Productivity measurements have been used to evaluate and compare physicians and physician practices. Anesthesiology is unique in that factors outside anesthesiologist control impact opportunity for revenue generation and make comparisons between providers and facilities challenging. This article uses data from the multicenter University of Pittsburgh Physicians Department of Anesthesiology to demonstrate factors influencing productivity opportunity by surgical facility, between department divisions and subspecialties within multispecialty divisions, and by individuals within divisions. The complexities of benchmarking anesthesiology productivity are demonstrated, and the potential value of creating a productivity profile for facilities and groups is illustrated. Copyright © 2018 Elsevier Inc. All rights reserved.

A 400 Gbps/100 m free-space optical link

NASA Astrophysics Data System (ADS)

Lin, Chun-Yu; Lu, Hai-Han; Ho, Chun-Ming; Cheng, Ming-Te; Huang, Sheng-Jhe; Wang, Yun-Chieh; Chi, Jing-Kai

2017-02-01

A 400 Gbps/100 m free-space optical (FSO) link with dense-wavelength-division-multiplexing (DWDM)/space-division-multiplexing (SDM) techniques and a doublet lens scheme is proposed. To the best of our knowledge, this is the first time that a link adopting DWDM and SDM techniques and a doublet lens scheme has demonstrated a 400 Gbps/100 m FSO link. The experimental results show that the free-space transmission rate is significantly enhanced by the DWDM and SDM techniques, and the free-space transmission distance is greatly increased by the doublet lens scheme. A 16-channel FSO link with a total transmission rate of 400 Gbps (25 Gbps/λ  ×  16 λ  =  400 Gbps) over a 100 m free-space link is successfully demonstrated. Such a 400 Gbps/100 m DWDM/SDM FSO link provides the advantages of optical wireless communications for high transmission rates and long transmission distances, which is very useful for high-speed and long-haul light-based WiFi (LiFi) applications.

An Autonomous Satellite Time Synchronization System Using Remotely Disciplined VC-OCXOs

PubMed Central

Gu, Xiaobo; Chang, Qing; Glennon, Eamonn P.; Xu, Baoda; Dempseter, Andrew G.; Wang, Dun; Wu, Jiapeng

2015-01-01

An autonomous remote clock control system is proposed to provide time synchronization and frequency syntonization for satellite to satellite or ground to satellite time transfer, with the system comprising on-board voltage controlled oven controlled crystal oscillators (VC-OCXOs) that are disciplined to a remote master atomic clock or oscillator. The synchronization loop aims to provide autonomous operation over extended periods, be widely applicable to a variety of scenarios and robust. A new architecture comprising the use of frequency division duplex (FDD), synchronous time division (STDD) duplex and code division multiple access (CDMA) with a centralized topology is employed. This new design utilizes dual one-way ranging methods to precisely measure the clock error, adopts least square (LS) methods to predict the clock error and employs a third-order phase lock loop (PLL) to generate the voltage control signal. A general functional model for this system is proposed and the error sources and delays that affect the time synchronization are discussed. Related algorithms for estimating and correcting these errors are also proposed. The performance of the proposed system is simulated and guidance for selecting the clock is provided. PMID:26213929

OPC-12759 increases proliferation of cultured rat conjunctival goblet cells.

PubMed

Ríos, José D; Shatos, Marie; Urashima, Hiroki; Tran, Hao; Dartt, Darlene A

2006-06-01

To determine if the gastroprotective drug OPC-12759 increased proliferation of rat conjunctival goblet cells in culture. Cultured goblet cells were incubated with 10(-12) to 10(-8) M OPC-12759 for 1 to 7 days. Fetal bovine serum (FBS) was used as a positive control. Cell proliferation was determined by a MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] colorimetric assay and by immunohistochemical staining with anti-Ki-67, a marker of cell division. Goblet cells were identified by double-labeling with anti-Ki-67, a marker of cell division, and Ulex europaeus agglutinin I lectin, anti-MUC5AC and anticytokeratin 7. Stratified squamous cells were identified by using Griffonia (Bandeiraea) simplicifolia lectin and anticytokeratin 4 antibody. As determined by MTT conversion to formazan, OPC-12579 at 10(-11) M induced an almost 2-fold increase in goblet cell proliferation on Days 1 and 3 of incubation but not on Days 5 and 7. The FBS at 10% increased cell proliferation by 2- to 3-fold at each time point. Daily replenishment of OPC-12579 for 3 consecutive days induced cell proliferation at all concentrations. Proliferation as determined by the number of Ki-67 positive cells increased by 4- and 3-fold at Days 1 and 3, respectively with addition of 10(-11) M OPC-12579. The FBS at 10% induced a 10-fold increase in goblet cell proliferation on Days 1, 3, and 5. Colocalization of Ulex europaeus agglutinin I, MUC5AC and anticytokeratin 7 with Ki-67 indicated that proliferating cells were goblet cells. Proliferating cells were negative for the nongoblet cell markers Bandeiraea lectin and anticytokeratin 4. The OPC-12759 stimulates proliferation of conjunctival goblet cells in primary culture.

Hybrid silica coarse wavelength-division multiplexer transmitter optical subassembly

NASA Astrophysics Data System (ADS)

An, Jun-Ming; Zhang, Jia-Shun; Wang, Liang-Liang; Zhu, Kaiwu; Sun, Bingli; Li, Yong; Hou, Jie; Li, Jian-Guang; Wu, Yuan-Da; Wang, Yue; Yin, Xiao-Jie

2018-01-01

Based on silica arrayed waveguide grating technology, a hybrid integrated transmitter optical subassembly was developed. Four direct-modulating distributed feedback lasers and four focusing microlenses were integrated to a coarse wavelength-division multiplexer (CWDM) on a CuW substrate. The four-channel silica-on-silicon CWDM was fabricated with 1.5% refractive index difference and 20-nm wavelength spacing. The experimental results showed that the output optical power was >3 mW with 45 mA of injection current, the slope efficiency was >0.0833 W/A, and the 3-dB bandwidth was broader than 18.15 GHz. The 1-dB compress points were higher than 18 and 15.8 dBm for frequency of 10 and 18 GHz, respectively.

Gender Differences in NATO Anthropometry and the Implication for Protective Equipment

DTIC Science & Technology

2008-09-01

National Research Council 2800 Q Street Wright-Patterson AFB OH 45433-7947 Kathleen M. Robinette Biomechanics Branch Biosciences and Protection...Air Force Research Laboratory Human Effectiveness Directorate Biosciences and Protection Division Biomechanics ...BARRY REEDER, Work Unit Manager MARK M. HOFFMAN, Deputy Chief Biomechanics

MECHANISMS OF ZN-INDUCED SIGNAL INITIATION THROUGH THE EPIDERMAL GROWTH FACTOR RECEPTOR (EGFR)

EPA Science Inventory

MECHANISMS OF Zn-INDUCED SIGNAL INITIATION THROUGH THE EPIDERMAL GROWTH FACTOR RECEPTOR (EGFR)
James M. Samet*, Lee M. Graves? and Weidong Wu?. *Human Studies Division, NHEERL, ORD, Research Triangle Park, NC 27711, and ?Center for Environmental Medicine, University of North C...

75 FR 79010 - National Cancer Institute; Notice of Closed Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-17

... DEPARTMENT OF HEALTH AND HUMAN SERVICES National Institutes of Health National Cancer Institute... Cancer Institute Special Emphasis Panel Education. Date: January 25, 2011. Time: 3 p.m. to 4:30 p.m..., Resources and Training Review Branch, Division of Extramural Activities, National Cancer Institute, 6116...

Publications - RI 2006-2 v. 1.0.1 | Alaska Division of Geological &

Science.gov Websites

, R.J., Werdon, M.B., Freeman, L.K., Smith, R.L., and Szumigala, D.J. Publication Date: Nov 2006 Bibliographic Reference Athey, J.E., Newberry, R.J., Werdon, M.B., Freeman, L.K., Smith, R.L., and Szumigala

76 FR 20375 - Meetings of Humanities Panel

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-12

...., Washington, DC 20506. FOR FURTHER INFORMATION CONTACT: Michael P. McDonald, Advisory Committee Management.... Time: 9 a.m. to 5 p.m. Location: Room 315. Program: This meeting will review applications for Landmark Workshops for School Teachers and Community College Faculty, submitted to the Division of Education Programs...

Hematopoietic stem cells can differentiate into restricted myeloid progenitors before cell division in mice.

PubMed

Grinenko, Tatyana; Eugster, Anne; Thielecke, Lars; Ramasz, Beáta; Krüger, Anja; Dietz, Sevina; Glauche, Ingmar; Gerbaulet, Alexander; von Bonin, Malte; Basak, Onur; Clevers, Hans; Chavakis, Triantafyllos; Wielockx, Ben

2018-05-15

Hematopoietic stem cells (HSCs) continuously replenish all blood cell types through a series of differentiation steps and repeated cell divisions that involve the generation of lineage-committed progenitors. However, whether cell division in HSCs precedes differentiation is unclear. To this end, we used an HSC cell-tracing approach and Ki67 RFP knock-in mice, in a non-conditioned transplantation model, to assess divisional history, cell cycle progression, and differentiation of adult HSCs. Our results reveal that HSCs are able to differentiate into restricted progenitors, especially common myeloid, megakaryocyte-erythroid and pre-megakaryocyte progenitors, without undergoing cell division and even before entering the S phase of the cell cycle. Additionally, the phenotype of the undivided but differentiated progenitors correlated with the expression of lineage-specific genes and loss of multipotency. Thus HSC fate decisions can be uncoupled from physical cell division. These results facilitate a better understanding of the mechanisms that control fate decisions in hematopoietic cells.

Direct interaction of FtsZ and MreB is required for septum synthesis and cell division in Escherichia coli.

PubMed

Fenton, Andrew K; Gerdes, Kenn

2013-07-03

How bacteria coordinate cell growth with division is not well understood. Bacterial cell elongation is controlled by actin-MreB while cell division is governed by tubulin-FtsZ. A ring-like structure containing FtsZ (the Z ring) at mid-cell attracts other cell division proteins to form the divisome, an essential protein assembly required for septum synthesis and cell separation. The Z ring exists at mid-cell during a major part of the cell cycle without contracting. Here, we show that MreB and FtsZ of Escherichia coli interact directly and that this interaction is required for Z ring contraction. We further show that the MreB-FtsZ interaction is required for transfer of cell-wall biosynthetic enzymes from the lateral to the mature divisome, allowing cells to synthesise the septum. Our observations show that bacterial cell division is coupled to cell elongation via a direct and essential interaction between FtsZ and MreB.

Activity and Accumulation of Cell Division-Promoting Phenolics in Tobacco Tissue Cultures 1

PubMed Central

Teutonico, Rita A.; Dudley, Matthew W.; Orr, John D.; Lynn, David G.; Binns, Andrew N.

1991-01-01

Dehydrodiconiferyl alcohol glucosides (DCGs) are derivatives of the phenylpropanoid pathway that have been isolated from Catharansus roseus L. (Vinca rosea) crown gall tumors. Fractions containing purified DCGs have been shown previously to promote the growth of cytokinin-requiring tissues of tobacco in the absence of exogenous cytokinins. In this study, we utilized synthetic DCG isomers to confirm the cell division-promoting activity of DCG isomers A and B and show that they neither promote shoot meristem initiation on Nicotiana tabacum L., cv Havana 425, leaf explants nor induce betacyanin synthesis in amaranth seedlings. Analysis of cultured tobacco pith tissue demonstrated that DCG accumulation was stimulated by cytokinin treatment and correlated with cytokinin-induced cell division. Thus, the accumulation of metabolites that could replace cytokinin in cell division bioassays is stimulated by cytokinins. These data support the model that DCGs are a component of a cytokinin-mediated regulatory circuit controlling cell division. ImagesFigure 2 PMID:16668384

Direct interaction of FtsZ and MreB is required for septum synthesis and cell division in Escherichia coli

PubMed Central

Fenton, Andrew K; Gerdes, Kenn

2013-01-01

How bacteria coordinate cell growth with division is not well understood. Bacterial cell elongation is controlled by actin–MreB while cell division is governed by tubulin–FtsZ. A ring-like structure containing FtsZ (the Z ring) at mid-cell attracts other cell division proteins to form the divisome, an essential protein assembly required for septum synthesis and cell separation. The Z ring exists at mid-cell during a major part of the cell cycle without contracting. Here, we show that MreB and FtsZ of Escherichia coli interact directly and that this interaction is required for Z ring contraction. We further show that the MreB–FtsZ interaction is required for transfer of cell-wall biosynthetic enzymes from the lateral to the mature divisome, allowing cells to synthesise the septum. Our observations show that bacterial cell division is coupled to cell elongation via a direct and essential interaction between FtsZ and MreB. PMID:23756461

Weapon System Software Acquisition and Support: A Theory of System Structure and Behavior.

DTIC Science & Technology

1982-03-01

Labs, Raytheon, FMC Inorganic Chemicals Division, Motorola Military Electroncs, Hughes Aircraft, General Dynamics/Fort Worth, Grumman and IBM, among...Organization Engineering Manpower M-16A Engineers Authorized ENGEN Expected Nunber of M-17 Engineers Engineers LEI Level of Engineers M-17A Engineers...productivity sector are listed below: ENGEX.K=ENGEX.J+DT(ENGCT.JK-REXEL.JK -RREA.JK) M-21,Level ENGEN =LEI M-17N,Initial ENGTOT=LEI M-18N,Initial ENGEX=LEI M

OSD1 promotes meiotic progression via APC/C inhibition and forms a regulatory network with TDM and CYCA1;2/TAM.

PubMed

Cromer, Laurence; Heyman, Jefri; Touati, Sandra; Harashima, Hirofumi; Araou, Emilie; Girard, Chloe; Horlow, Christine; Wassmann, Katja; Schnittger, Arp; De Veylder, Lieven; Mercier, Raphael

2012-01-01

Cell cycle control is modified at meiosis compared to mitosis, because two divisions follow a single DNA replication event. Cyclin-dependent kinases (CDKs) promote progression through both meiosis and mitosis, and a central regulator of their activity is the APC/C (Anaphase Promoting Complex/Cyclosome) that is especially required for exit from mitosis. We have shown previously that OSD1 is involved in entry into both meiosis I and meiosis II in Arabidopsis thaliana; however, the molecular mechanism by which OSD1 controls these transitions has remained unclear. Here we show that OSD1 promotes meiotic progression through APC/C inhibition. Next, we explored the functional relationships between OSD1 and the genes known to control meiotic cell cycle transitions in Arabidopsis. Like osd1, cyca1;2/tam mutation leads to a premature exit from meiosis after the first division, while tdm mutants perform an aberrant third meiotic division after normal meiosis I and II. Remarkably, while tdm is epistatic to tam, osd1 is epistatic to tdm. We further show that the expression of a non-destructible CYCA1;2/TAM provokes, like tdm, the entry into a third meiotic division. Finally, we show that CYCA1;2/TAM forms an active complex with CDKA;1 that can phosphorylate OSD1 in vitro. We thus propose that a functional network composed of OSD1, CYCA1;2/TAM, and TDM controls three key steps of meiotic progression, in which OSD1 is a meiotic APC/C inhibitor.

An Evaluation of Sea Turtle Populations and Survival Status on Vieques Island.

DTIC Science & Technology

1982-06-22

Pritchard T. H. Stubbs Florida Audubon Society (N66001-80-C-0560) 22 June 1982 Prepared for Marine Sciences Division Approved for public release...the Florida Audubon Society for NOSC Marine Sciences Division (Code 513). I Released by Under authority of S. Yamamoto. Head H.O. Porter. Head Marine ...Coollco so wo . i .0*W ow a AomY b block4 m.. Reptiles -. HawksbilIVieques Isand Loaesbsd Green turtle Nestn Turtles Loatharback 2 0. AGSTA ACT

Influence of Organizational Culture on the Acquisition of the M16 Rifle

DTIC Science & Technology

2006-12-15

plan was developed named “ Broiler ” which began to integrate atomic weapons into the nation’s security strategy. By 1948 the emphasis continued to...38 Ibid., 37. 39 Ibid., 37-38. 40 Patrick Sweeney, The Gun Digest Book of the AR-15 (Iola, WI: KP Books, A Division of F+W Publicaitons, 2005), 10...Patrick. 2005. The Gun Digest Book of the AR-15. Iola, WI: KP Books, A Division of F+W Publicaitons. Whetten, David A., and Kim S. Cameron. 2002