Accumulation of neutral mutations in growing cell colonies with competition.

PubMed

Sorace, Ron; Komarova, Natalia L

2012-12-07

Neutral mutations play an important role in many biological processes including cancer initiation and progression, the generation of drug resistance in bacterial and viral diseases as well as cancers, and the development of organs in multicellular organisms. In this paper we study how neutral mutants are accumulated in nonlinearly growing colonies of cells subject to growth constraints such as crowding or lack of resources. We investigate different types of growth control which range from "division-controlled" to "death-controlled" growth (and various mixtures of both). In division-controlled growth, the burden of handling overcrowding lies with the process of cell-divisions, the divisions slow down as the carrying capacity is approached. In death-controlled growth, it is death rate that increases to slow down expansion. We show that division-controlled growth minimizes the number of accumulated mutations, and death-controlled growth corresponds to the maximum number of mutants. We check that these results hold in both deterministic and stochastic settings. We further develop a general (deterministic) theory of neutral mutations and achieve an analytical understanding of the mutant accumulation in colonies of a given size in the absence of back-mutations. The long-term dynamics of mutants in the presence of back-mutations is also addressed. In particular, with equal forward- and back-mutation rates, if division-controlled and a death-controlled types are competing for space and nutrients, cells obeying division-controlled growth will dominate the population. Copyright © 2012 Elsevier Ltd. All rights reserved.

Instrumentation and Controls Division progress report, July 1, 1990--June 30, 1992. Volume 2

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1993-01-01

This report contains the following information from the Instrumentation and Controls Division of Oak Ridge National Laboratory: supplementary activities; seminars; publications and presentations; scientific and professional activities, achievements, and awards; and division organization charts.

Investigating Autonomic Control of the Cardiovascular System: A Battery of Simple Tests

ERIC Educational Resources Information Center

Johnson, Christopher D.; Roe, Sean; Tansey, Etain A.

2013-01-01

Sympathetic and parasympathetic divisions of the autonomic nervous system constantly control the heart (sympathetic and parasympathetic divisions) and blood vessels (predominantly the sympathetic division) to maintain appropriate blood pressure and organ blood flow over sometimes widely varying conditions. This can be adversely affected by…

Division 1137 property control system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pastor, D.J.

1982-01-01

An automated data processing property control system was developed by Mobile and Remote Range Division 1137. This report describes the operation of the system and examines ways of using it in operational planning and control.

40 CFR 52.1977 - Content of approved State submitted implementation plan.

Code of Federal Regulations, 2013 CFR

2013-07-01

...) Division 28—Stationary Source Air Pollution Control and Permitting Procedures Excess Emissions and... Requirements (11/4/93) 28-1450Enforcement Action Criteria (9/24/93) Division 200—General Air Pollution... Designations (10/14/99) 204-0090Oxygenated Gasoline Control Areas (3/27/01) Division 206—Air Pollution...

40 CFR 52.1977 - Content of approved State submitted implementation plan.

Code of Federal Regulations, 2012 CFR

2012-07-01

...) Division 28—Stationary Source Air Pollution Control and Permitting Procedures Excess Emissions and... Requirements (11/4/93) 28-1450Enforcement Action Criteria (9/24/93) Division 200—General Air Pollution... Designations (10/14/99) 204-0090Oxygenated Gasoline Control Areas (3/27/01) Division 206—Air Pollution...

40 CFR 52.1977 - Content of approved State submitted implementation plan.

Code of Federal Regulations, 2014 CFR

2014-07-01

...) Division 28—Stationary Source Air Pollution Control and Permitting Procedures Excess Emissions and... Requirements (11/4/93) 28-1450Enforcement Action Criteria (9/24/93) Division 200—General Air Pollution... Designations (10/14/99) 204-0090Oxygenated Gasoline Control Areas (3/27/01) Division 206—Air Pollution...

40 CFR 52.1977 - Content of approved State submitted implementation plan.

Code of Federal Regulations, 2011 CFR

2011-07-01

...) Division 28—Stationary Source Air Pollution Control and Permitting Procedures Excess Emissions and... Requirements (11/4/93) 28-1450Enforcement Action Criteria (9/24/93) Division 200—General Air Pollution... Designations (10/14/99) 204-0090Oxygenated Gasoline Control Areas (3/27/01) Division 206—Air Pollution...

IFLA General Conference, 1991. Division of Bibliographic Control: Open Forum of Division of Bibliographic Control; Section of Cataloguing; Section of Bibliography; Section of Classification and Indexing. Booklet 4.

ERIC Educational Resources Information Center

International Federation of Library Associations and Institutions, The Hague (Netherlands).

The 11 reports and papers in this booklet were presented at meetings of 4 sections within the Division of Bibliographic Control: (1) "Report of the Section on Cataloguing--Review of the Work 1990/1991" (Inger Cathrine Spangen, Norway); (2) "Les fichiers d'autorite auteurs: Rapport d'activite 1990-1991 (Author Authority Lists: Report…

7 CFR 94.101 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-01-01

... applicant for chemical, physical, or microbiological analyses and tests at a Science and Technology Division... Science and Technology Division laboratory, or by a laboratory approved and recognized by the Division to... quality control of procedures. Official plant or Science and Technology Division laboratories can analyze...

7 CFR 94.101 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-01-01

... applicant for chemical, physical, or microbiological analyses and tests at a Science and Technology Division... Science and Technology Division laboratory, or by a laboratory approved and recognized by the Division to... quality control of procedures. Official plant or Science and Technology Division laboratories can analyze...

7 CFR 94.101 - Definitions.

Code of Federal Regulations, 2012 CFR

2012-01-01

... applicant for chemical, physical, or microbiological analyses and tests at a Science and Technology Division... Science and Technology Division laboratory, or by a laboratory approved and recognized by the Division to... quality control of procedures. Official plant or Science and Technology Division laboratories can analyze...

RESEARCH AREA -- ARTIFICIAL INTELLIGENCE CONTROL (AIR POLLUTION TECHNOLOGY BRANCH, AIR POLLUTION PREVENTION AND CONTROL DIVISION, NRMRL)

EPA Science Inventory

The Air Pollution Technology Branch (APTB) of NRMRL's Air Pollution Prevention and Control Division in Research Triangle Park, NC, has conducted several research projects for evaluating the use of artificial intelligence (AI) to improve the control of pollution control systems an...

The C. elegans engrailed homolog ceh-16 regulates the self-renewal expansion division of stem cell-like seam cells.

PubMed

Huang, Xinxin; Tian, E; Xu, Yanhua; Zhang, Hong

2009-09-15

Stem cells undergo symmetric and asymmetric division to maintain the dynamic equilibrium of the stem cell pool and also to generate a variety of differentiated cells. The homeostatic mechanism controlling the choice between self-renewal and differentiation of stem cells is poorly understood. We show here that ceh-16, encoding the C. elegans ortholog of the transcription factor Engrailed, controls symmetric and asymmetric division of stem cell-like seam cells. Loss of function of ceh-16 causes certain seam cells, which normally undergo symmetric self-renewal expansion division with both daughters adopting the seam cell fate, to divide asymmetrically with only one daughter retaining the seam cell fate. The human engrailed homolog En2 functionally substitutes the role of ceh-16 in promoting self-renewal expansion division of seam cells. Loss of function of apr-1, encoding the C. elegans homolog of the Wnt signaling component APC, results in transformation of self-renewal maintenance seam cell division to self-renewal expansion division, leading to seam cell hyperplasia. The apr-1 mutation suppresses the seam cell division defect in ceh-16 mutants. Our study reveals that ceh-16 interacts with the Wnt signaling pathway to control the choice between self-renewal expansion and maintenance division and also demonstrates an evolutionarily conserved function of engrailed in promoting cell proliferation.

Auxin-Dependent Cell Division and Cell Elongation. 1-Naphthaleneacetic Acid and 2,4-Dichlorophenoxyacetic Acid Activate Different Pathways1

PubMed Central

Campanoni, Prisca; Nick, Peter

2005-01-01

During exponential phase, the tobacco (Nicotiana tabacum) cell line cv Virginia Bright Italia-0 divides axially to produce linear cell files of distinct polarity. This axial division is controlled by exogenous auxin. We used exponential tobacco cv Virginia Bright Italia-0 cells to dissect early auxin signaling, with cell division and cell elongation as physiological markers. Experiments with 1-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D) demonstrated that these 2 auxin species affect cell division and cell elongation differentially; NAA stimulates cell elongation at concentrations that are much lower than those required to stimulate cell division. In contrast, 2,4-D promotes cell division but not cell elongation. Pertussis toxin, a blocker of heterotrimeric G-proteins, inhibits the stimulation of cell division by 2,4-D but does not affect cell elongation. Aluminum tetrafluoride, an activator of the G-proteins, can induce cell division at NAA concentrations that are not permissive for division and even in the absence of any exogenous auxin. The data are discussed in a model where the two different auxins activate two different pathways for the control of cell division and cell elongation. PMID:15734918

Biorepositories | Division of Cancer Prevention

Cancer.gov

Carefully collected and controlled high-quality human biospecimens, annotated with clinical data and properly consented for investigational use, are available through the Division of Cancer Prevention Biorepositories listed in the charts below. Biorepositories Managed by the Division of Cancer Prevention Biorepositories Supported by the Division of Cancer Prevention Related

Instrumentation and Controls Division Overview: Sensors Development for Harsh Environments at Glenn Research Center

NASA Technical Reports Server (NTRS)

Zeller, Mary V.; Lei, Jih-Fen

2002-01-01

The Instrumentation and Controls Division is responsible for planning, conducting and directing basic and applied research on advanced instrumentation and controls technologies for aerospace propulsion and power applications. The Division's advanced research in harsh environment sensors, high temperature high power electronics, MEMS (microelectromechanical systems), nanotechnology, high data rate optical instrumentation, active and intelligent controls, and health monitoring and management will enable self-feeling, self-thinking, self-reconfiguring and self-healing Aerospace Propulsion Systems. These research areas address Agency challenges to deliver aerospace systems with reduced size and weight, and increased functionality and intelligence for future NASA missions in advanced aeronautics, economical space transportation, and pioneering space exploration. The Division also actively supports educational and technology transfer activities aimed at benefiting all humankind.

Quality of life and objective outcome assessment in women with tape division after surgery for stress urinary incontinence

PubMed Central

Ulrich, Daniela; Bjelic-Radisic, Vesna; Höllein, Anna; Tamussino, Karl; Aigmüller, Thomas

2017-01-01

Background Midurethral tapes may cause long-term complications such as voiding dysfunction, groin pain, de novo urgency or mesh erosion, which necessitate a reoperation. There is a paucity of data regarding health related quality of life in patients undergoing tape removal. The aim of the study was to evaluate quality of life (QoL) and objective outcome after midurethral tape division or excision. Methods All patients who underwent a midurethral tape division for voiding difficulties, pain or therapy resistant de novo overactive bladder between 1999 and 2014 were invited for follow-up. A control group with a suburethral tape without division was established in a 1:2 ratio and matched for age, tape used and year of tape insertion. Patients completed the Kings´ Health Questionnaire (KHQ), Incontinence Outcome Questionnaire, Female Sexual Function Index Questionnaire and the Patient Global Impression of Improvement score. Results Tape division or excision was performed in 32 women. Overall, 15 (60%) of 25 women who were alive were available for clinical examination and completed the questionnaires. Tape division was performed for voiding dysfunction (n = 7), overactive bladder (n = 2), mesh extrusion (n = 3) and ongoing pain (n = 3). Median time to tape division/excision was 10 months. Three women in the tape division group had undergone reoperation for stress urinary incontinence (SUI). At a median follow-up of 11 years (IQR 9–13) subjective SUI rate was 53% (8/15 women) in the tape division group and 17% (5/30) in the control group (p = 0.016), with no significant differences in objective SUI rates between groups. With regard to quality of life, the study group had significantly worse scores in the SUI related domains role limitation, physical limitation, severity measures and social limitations (KHQ) compared to the control group. Conclusions Women needing tape division or excision have lower SUI related QoL scores compared to controls mostly because of higher subjective SUI rates. PMID:28346541

Robust control of mitotic spindle orientation in the developing epidermis

PubMed Central

Poulson, Nicholas D.

2010-01-01

Progenitor cells must balance self-amplification and production of differentiated progeny during development and homeostasis. In the epidermis, progenitors divide symmetrically to increase surface area and asymmetrically to promote stratification. In this study, we show that individual epidermal cells can undergo both types of division, and therefore, the balance is provided by the sum of individual cells’ choices. In addition, we define two control points for determining a cell’s mode of division. First is the expression of the mouse Inscuteable gene, which is sufficient to drive asymmetric cell division (ACD). However, there is robust control of division orientation as excessive ACDs are prevented by a change in the localization of NuMA, an effector of spindle orientation. Finally, we show that p63, a transcriptional regulator of stratification, does not control either of these processes. These data have uncovered two important regulatory points controlling ACD in the epidermis and allow a framework for analysis of how external cues control this important choice. PMID:21098114

IFLA General Conference, 1989. Division of Bibliographic Control. Open Forum of the Division; Section on Cataloguing; Section on Bibliography; Section on Classification and Indexing. Booklet 40.

ERIC Educational Resources Information Center

International Federation of Library Associations, The Hague (Netherlands).

There are 16 papers in this collection from the Division of Bibliographic Control: (1) "Report from the Section on Cataloging" (Tom Delsey); (2) "Section on Bibliography Report" (B. C. Bloomfield); (3) "Report from the Section on Classification and Indexing" (Robert P. Holley); (4) "The Cost of Cataloging" (French and English versions; Genevieve…

75 FR 24748 - Johnson Controls, Inc., Automotive Experience Division, Including Workers Whose Unemployment...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-05

...., Automotive Experience Division, Including Workers Whose Unemployment Insurance (UI) Wages Are Paid Through... Assistance on October 6, 2009, applicable to workers of Johnson Controls, Inc., Automotive Experience... industry. New information shows that Johnson Controls purchased Hoover Universal in 1985 and that some...

Investigation of controlled flight into terrain : descriptions of flight paths for selected controlled flight into terrain (CFIT) aircraft accidents, 1985-1997

DOT National Transportation Integrated Search

1999-03-01

This report documents an investigation of the flight paths of 13 selected controlled flight into terrain (CFIT) aircraft accidents that occurred between 1985 and 1997. The Operations Assessment Division (DTS-43) and the Aviation Safety Division (DTS-...

Instrumentation and Controls Division progress report for the period July 1, 1986 to June 30, 1988

DOE Office of Scientific and Technical Information (OSTI.GOV)

Klobe, L.E.

1988-12-01

The Instrumentation and Controls (IandC) Division of Oak Ridge National Laboratory (ORNL) performs basic and applied instrumentation and controls research, development and design engineering, specialized instrument design and fabrication, and maintenance services for instruments, electronics, and computers. The IandC Division is one of the largest RandD organizations of its type among government laboratories, and it exists as the result of an organizational strategy to integrate ORNL's instrumentation and controls-related disciplines into one dedicated functional organization to increase the Laboratory's expertise and capabilities in these rapidly expanding, innovative areas of technology. The Division participates in the programs and projects of ORNLmore » by applying its expertise and capabilities in concert with other divisions to perform basic research and mission-oriented technology development. Many of the Division's RandD tasks that are a part of a larger ORNL program are of sufficient scope that the IandC effort constitutes a separate program element with direct funding and management responsibility within the Division. The activities of IandC include performance of an RandD task in IandC facilities, the participation of from one of many IandC engineers and scientists in a multidisciplinary team working in a specific research area or development project, design and fabrication of a special instrument or instrumentation system, or a few hours of maintenance service. In its support and maintenance work, the role of the IandC Division is to provide a level of expertise appropriate to complete a job successfully at minimum overall cost and time schedule---a role which involves IandC in almost all ORNL activities.« less

Polarity, cell division, and out-of-equilibrium dynamics control the growth of epithelial structures

PubMed Central

Cerruti, Benedetta; Puliafito, Alberto; Shewan, Annette M.; Yu, Wei; Combes, Alexander N.; Little, Melissa H.; Chianale, Federica; Primo, Luca; Serini, Guido; Mostov, Keith E.; Celani, Antonio

2013-01-01

The growth of a well-formed epithelial structure is governed by mechanical constraints, cellular apico-basal polarity, and spatially controlled cell division. Here we compared the predictions of a mathematical model of epithelial growth with the morphological analysis of 3D epithelial structures. In both in vitro cyst models and in developing epithelial structures in vivo, epithelial growth could take place close to or far from mechanical equilibrium, and was determined by the hierarchy of time-scales of cell division, cell–cell rearrangements, and lumen dynamics. Equilibrium properties could be inferred by the analysis of cell–cell contact topologies, and the nonequilibrium phenotype was altered by inhibiting ROCK activity. The occurrence of an aberrant multilumen phenotype was linked to fast nonequilibrium growth, even when geometric control of cell division was correctly enforced. We predicted and verified experimentally that slowing down cell division partially rescued a multilumen phenotype induced by altered polarity. These results improve our understanding of the development of epithelial organs and, ultimately, of carcinogenesis. PMID:24145168

Focusing light through random scattering media by four-element division algorithm

NASA Astrophysics Data System (ADS)

Fang, Longjie; Zhang, Xicheng; Zuo, Haoyi; Pang, Lin

2018-01-01

The focusing of light through random scattering materials using wavefront shaping is studied in detail. We propose a newfangled approach namely four-element division algorithm to improve the average convergence rate and signal-to-noise ratio of focusing. Using 4096 independently controlled segments of light, the intensity at the target is 72 times enhanced over the original intensity at the same position. The four-element division algorithm and existing phase control algorithms of focusing through scattering media are compared by both of the numerical simulation and the experiment. It is found that four-element division algorithm is particularly advantageous to improve the average convergence rate of focusing.

33 CFR 274.6 - Division/district pest control programs.

Code of Federal Regulations, 2010 CFR

2010-07-01

..., DEPARTMENT OF DEFENSE PEST CONTROL PROGRAM FOR CIVIL WORKS PROJECTS Project Operation § 274.6 Division... from time to time, will be used as guides in selecting the type of chemicals and the method of application in the control of vegetation and pests at civil works projects. (b) Responsibilities and reports...

Metabolism and the Control of Cell Fate Decisions and Stem Cell Renewal

PubMed Central

Ito, Kyoko; Ito, Keisuke

2016-01-01

Although the stem cells of various tissues remain in the quiescent state to maintain their undifferentiated state, they also undergo cell divisions as required, and if necessary, even a single stem cell is able to provide for lifelong tissue homeostasis. Stem cell populations are precisely controlled by the balance between their symmetric and asymmetric divisions, with their division patterns determined by whether the daughter cells involved retain their self-renewal capacities. Recent studies have reported that metabolic pathways and the distribution of mitochondria are regulators of the division balance of stem cells and that metabolic defects can shift division balance toward symmetric commitment, which leads to stem cell exhaustion. It has also been observed that in asymmetric division, old mitochondria, which are central metabolic organelles, are segregated to the daughter cell fated to cell differentiation, whereas in symmetric division, young and old mitochondria are equally distributed between both daughter cells. Thus, metabolism and mitochondrial biology play important roles in stem cell fate decisions. As these decisions directly affect tissue homeostasis, understanding their regulatory mechanisms in the context of cellular metabolism is critical. PMID:27482603

Metabolism and the Control of Cell Fate Decisions and Stem Cell Renewal.

PubMed

Ito, Kyoko; Ito, Keisuke

2016-10-06

Although the stem cells of various tissues remain in the quiescent state to maintain their undifferentiated state, they also undergo cell divisions as required, and if necessary, even a single stem cell is able to provide for lifelong tissue homeostasis. Stem cell populations are precisely controlled by the balance between their symmetric and asymmetric divisions, with their division patterns determined by whether the daughter cells involved retain their self-renewal capacities. Recent studies have reported that metabolic pathways and the distribution of mitochondria are regulators of the division balance of stem cells and that metabolic defects can shift division balance toward symmetric commitment, which leads to stem cell exhaustion. It has also been observed that in asymmetric division, old mitochondria, which are central metabolic organelles, are segregated to the daughter cell fated to cell differentiation, whereas in symmetric division, young and old mitochondria are equally distributed between both daughter cells. Thus, metabolism and mitochondrial biology play important roles in stem cell fate decisions. As these decisions directly affect tissue homeostasis, understanding their regulatory mechanisms in the context of cellular metabolism is critical.

PUBLICATIONS (AIR POLLUTION TECHNOLOGY BRANCH, AIR POLLUTION PREVENTION AND CONTROL DIVISION, NRMRL)

EPA Science Inventory

The Air Pollution Technology Branch (APTB) of NRMRL's Air Pollution Prevention and Control Division produces and publishes highly specialized technical and scientific documents related to APTB's research. Areas of research covered include artificial intelligence, CFC destruction,...

GREENHOUSE GASES (ATMOSPHERIC PROTECTION BRANCH, AIR POLLUTION PREVENTION AND CONTROL DIVISION, NRMRL)

EPA Science Inventory

Greenhouse gas (GHG) emissions are projected for various scenarios and the most appropriate approaches and technologies for mitigation are identified by NRMRL's Air Pollution Prevention and Control Division's Atmospheric Protection Branch (APB). These methods contribute to reduct...

Oriented cell division: new roles in guiding skin wound repair and regeneration

PubMed Central

Yang, Shaowei; Ma, Kui; Geng, Zhijun; Sun, Xiaoyan; Fu, Xiaobing

2015-01-01

Tissue morphogenesis depends on precise regulation and timely co-ordination of cell division and also on the control of the direction of cell division. Establishment of polarity division axis, correct alignment of the mitotic spindle, segregation of fate determinants equally or unequally between daughter cells, are essential for the realization of oriented cell division. Furthermore, oriented cell division is regulated by intrinsic cues, extrinsic cues and other cues, such as cell geometry and polarity. However, dysregulation of cell division orientation could lead to abnormal tissue development and function. In the present study, we review recent studies on the molecular mechanism of cell division orientation and explain their new roles in skin repair and regeneration. PMID:26582817

Mechanical stretch triggers rapid epithelial cell division through Piezo1.

PubMed

Gudipaty, S A; Lindblom, J; Loftus, P D; Redd, M J; Edes, K; Davey, C F; Krishnegowda, V; Rosenblatt, J

2017-03-02

Despite acting as a barrier for the organs they encase, epithelial cells turn over at some of the fastest rates in the body. However, epithelial cell division must be tightly linked to cell death to preserve barrier function and prevent tumour formation. How does the number of dying cells match those dividing to maintain constant numbers? When epithelial cells become too crowded, they activate the stretch-activated channel Piezo1 to trigger extrusion of cells that later die. However, it is unclear how epithelial cell division is controlled to balance cell death at the steady state. Here we show that mammalian epithelial cell division occurs in regions of low cell density where cells are stretched. By experimentally stretching epithelia, we find that mechanical stretch itself rapidly stimulates cell division through activation of the Piezo1 channel. To stimulate cell division, stretch triggers cells that are paused in early G2 phase to activate calcium-dependent phosphorylation of ERK1/2, thereby activating the cyclin B transcription that is necessary to drive cells into mitosis. Although both epithelial cell division and cell extrusion require Piezo1 at the steady state, the type of mechanical force controls the outcome: stretch induces cell division, whereas crowding induces extrusion. How Piezo1-dependent calcium transients activate two opposing processes may depend on where and how Piezo1 is activated, as it accumulates in different subcellular sites with increasing cell density. In sparse epithelial regions in which cells divide, Piezo1 localizes to the plasma membrane and cytoplasm, whereas in dense regions in which cells extrude, it forms large cytoplasmic aggregates. Because Piezo1 senses both mechanical crowding and stretch, it may act as a homeostatic sensor to control epithelial cell numbers, triggering extrusion and apoptosis in crowded regions and cell division in sparse regions.

Subtractive, divisive and non-monotonic gain control in feedforward nets linearized by noise and delays.

PubMed

Mejias, Jorge F; Payeur, Alexandre; Selin, Erik; Maler, Leonard; Longtin, André

2014-01-01

The control of input-to-output mappings, or gain control, is one of the main strategies used by neural networks for the processing and gating of information. Using a spiking neural network model, we studied the gain control induced by a form of inhibitory feedforward circuitry-also known as "open-loop feedback"-, which has been experimentally observed in a cerebellum-like structure in weakly electric fish. We found, both analytically and numerically, that this network displays three different regimes of gain control: subtractive, divisive, and non-monotonic. Subtractive gain control was obtained when noise is very low in the network. Also, it was possible to change from divisive to non-monotonic gain control by simply modulating the strength of the feedforward inhibition, which may be achieved via long-term synaptic plasticity. The particular case of divisive gain control has been previously observed in vivo in weakly electric fish. These gain control regimes were robust to the presence of temporal delays in the inhibitory feedforward pathway, which were found to linearize the input-to-output mappings (or f-I curves) via a novel variability-increasing mechanism. Our findings highlight the feedforward-induced gain control analyzed here as a highly versatile mechanism of information gating in the brain.

Subtractive, divisive and non-monotonic gain control in feedforward nets linearized by noise and delays

PubMed Central

Mejias, Jorge F.; Payeur, Alexandre; Selin, Erik; Maler, Leonard; Longtin, André

2014-01-01

The control of input-to-output mappings, or gain control, is one of the main strategies used by neural networks for the processing and gating of information. Using a spiking neural network model, we studied the gain control induced by a form of inhibitory feedforward circuitry—also known as “open-loop feedback”—, which has been experimentally observed in a cerebellum-like structure in weakly electric fish. We found, both analytically and numerically, that this network displays three different regimes of gain control: subtractive, divisive, and non-monotonic. Subtractive gain control was obtained when noise is very low in the network. Also, it was possible to change from divisive to non-monotonic gain control by simply modulating the strength of the feedforward inhibition, which may be achieved via long-term synaptic plasticity. The particular case of divisive gain control has been previously observed in vivo in weakly electric fish. These gain control regimes were robust to the presence of temporal delays in the inhibitory feedforward pathway, which were found to linearize the input-to-output mappings (or f-I curves) via a novel variability-increasing mechanism. Our findings highlight the feedforward-induced gain control analyzed here as a highly versatile mechanism of information gating in the brain. PMID:24616694

Military Construction: FY2017 Appropriations

DTIC Science & Technology

2016-10-04

combined the versions of the Transportation, Housing and Urban Development (T-HUD), Military Construction and Veterans Affairs (MILCON/VA), and Zika ...amended bill, and sent it to the House. The House substituted its own amendment in three divisions (Division A: MILCON/VA, Division B: Zika Response...Appropriations, and Division C: Zika Vector Control), removing the T-HUD portion for H.R. 2577, passed the bill, and requested a conference. The

Constriction of the mitochondrial inner compartment is a priming event for mitochondrial division

PubMed Central

Cho, Bongki; Cho, Hyo Min; Jo, Youhwa; Kim, Hee Dae; Song, Myungjae; Moon, Cheil; Kim, Hyongbum; Kim, Kyungjin; Sesaki, Hiromi; Rhyu, Im Joo; Kim, Hyun; Sun, Woong

2017-01-01

Mitochondrial division is critical for the maintenance and regulation of mitochondrial function, quality and distribution. This process is controlled by cytosolic actin-based constriction machinery and dynamin-related protein 1 (Drp1) on mitochondrial outer membrane (OMM). Although mitochondrial physiology, including oxidative phosphorylation, is also important for efficient mitochondrial division, morphological alterations of the mitochondrial inner-membrane (IMM) have not been clearly elucidated. Here we report spontaneous and repetitive constriction of mitochondrial inner compartment (CoMIC) associated with subsequent division in neurons. Although CoMIC is potentiated by inhibition of Drp1 and occurs at the potential division spots contacting the endoplasmic reticulum, it appears on IMM independently of OMM. Intra-mitochondrial influx of Ca2+ induces and potentiates CoMIC, and leads to K+-mediated mitochondrial bulging and depolarization. Synergistically, optic atrophy 1 (Opa1) also regulates CoMIC via controlling Mic60-mediated OMM–IMM tethering. Therefore, we propose that CoMIC is a priming event for efficient mitochondrial division. PMID:28598422

A single-cell pedigree analysis of alternative stochastic lymphocyte fates

PubMed Central

Hawkins, E. D.; Markham, J. F.; McGuinness, L. P.; Hodgkin, P. D.

2009-01-01

In contrast to most stimulated lymphocytes, B cells exposed to Toll-like receptor 9 ligands are nonself-adherent, allowing individual cells and families to be followed in vitro for up to 5 days. These B cells undergo phases typical of an adaptive response, dividing up to 6 times before losing the impetus for further growth and division and eventually dying by apoptosis. Using long-term microscopic imaging, accurate histories of individual lymphocyte fates were collected. Quantitative analysis of family relationships revealed that times to divide of siblings were strongly related but these correlations were progressively lost through consecutive divisions. A weaker, but significant, correlation was also found for death times among siblings. Division cessation is characterized by a loss of cell growth and the division in which this occurs is strongly inherited from the original founder cell and is related to the size this cell reaches before its first division. Thus, simple division-based dilution of factors synthesized during the first division may control the maximum division reached by stimulated cells. The stochastic distributions of times to divide, times to die, and divisions reached are also measured. Together, these results highlight the internal cellular mechanisms that control immune responses and provide a foundation for the development of new mathematical models that are correct at both single-cell and population levels. PMID:19633185

THE IMPORTANCE OF A SUCCESSFUL QUALITY ASSURANCE (QA) PROGRAM FROM A RESEARCH MANAGER'S PERSPECTIVE

EPA Science Inventory

The paper discusses the Air Pollution Prevention and Control Division's Quality Assurance (QA) program and the approaches used to meet QA requirements in the Division. The presentation is a technical manager's perspective of the Division's requirements for and approach to QA in i...

New Cancer Prevention and Control Central Institutional Review Board Established | Division of Cancer Prevention

Cancer.gov

The NCI Central Institutional Review Board (CIRB) Initiative announced the establishment of the Cancer Prevention and Control (CPC) CIRB January 14, extending the benefits of centralized review to investigators participating in clinical trials sponsored by the Division of Cancer Prevention (DCP). |

40 CFR 52.2239 - Original Identification of plan section.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Original Identification of plan section. (a) This section identifies the original “Tennessee Air Pollution... on February 3, 1972, by the Division of Air Pollution Control of the Tennessee Department of Public... Division of Air Pollution Control of the Tennessee Department of Public Health. (3) Statements of intent...

Role of asymmetric cell division in lifespan control in Saccharomyces cerevisiae

PubMed Central

Higuchi-Sanabria, Ryo; Pernice, Wolfgang M A; Vevea, Jason D; Alessi Wolken, Dana M; Boldogh, Istvan R; Pon, Liza A

2014-01-01

Aging determinants are asymmetrically distributed during cell division in S. cerevisiae, which leads to production of an immaculate, age-free daughter cell. During this process, damaged components are sequestered and retained in the mother cell, and higher functioning organelles and rejuvenating factors are transported to and/or enriched in the bud. Here, we will describe the key quality control mechanisms in budding yeast that contribute to asymmetric cell division of aging determinants including mitochondria, endoplasmic reticulum (ER), vacuoles, extrachromosomal rDNA circles (ERCs), and protein aggregates. PMID:25263578

Control of proliferation and cancer growth by the Hippo signaling pathway

PubMed Central

Ehmer, Ursula; Sage, Julien

2015-01-01

The control of cell division is essential for normal development and the maintenance of cellular homeostasis. Abnormal cell proliferation is associated with multiple pathological states, including cancer. While the Hippo/YAP signaling pathway was initially thought to control organ size and growth, increasing evidence indicates that this pathway also plays a major role in the control of proliferation independent of organ size control. In particular, accumulating evidence indicates that the Hippo/YAP signaling pathway functionally interacts with multiple other cellular pathways and serves as a central node in the regulation of cell division, especially in cancer cells. Here recent observations are highlighted that connect Hippo/YAP signaling to transcription, the basic cell cycle machinery, and the control of cell division. Furthermore, the oncogenic and tumor suppressive attributes of YAP/TAZ are reviewed which emphasizes the relevance of the Hippo pathway in cancer. PMID:26432795

The Division of Labor in Lesbian, Gay, and Heterosexual New Adoptive Parents

ERIC Educational Resources Information Center

Goldberg, Abbie E.; Smith, JuliAnna Z.; Perry-Jenkins, Maureen

2012-01-01

Little research has investigated the division of child care and housework in adoptive or lesbian/gay parent families, yet these contexts "control for" family characteristics such as biological relatedness and parental gender differences known to be linked to family work. This study examined predictors (measured preadoption) of the division of…

A new class of cyclin dependent kinase in Chlamydomonas is required for coupling cell size to cell division

PubMed Central

Li, Yubing; Liu, Dianyi; López-Paz, Cristina; Olson, Bradley JSC; Umen, James G

2016-01-01

Proliferating cells actively control their size by mechanisms that are poorly understood. The unicellular green alga Chlamydomonas reinhardtii divides by multiple fission, wherein a ‘counting’ mechanism couples mother cell-size to cell division number allowing production of uniform-sized daughters. We identified a sizer protein, CDKG1, that acts through the retinoblastoma (RB) tumor suppressor pathway as a D-cyclin-dependent RB kinase to regulate mitotic counting. Loss of CDKG1 leads to fewer mitotic divisions and large daughters, while mis-expression of CDKG1 causes supernumerous mitotic divisions and small daughters. The concentration of nuclear-localized CDKG1 in pre-mitotic cells is set by mother cell size, and its progressive dilution and degradation with each round of cell division may provide a link between mother cell-size and mitotic division number. Cell-size-dependent accumulation of limiting cell cycle regulators such as CDKG1 is a potentially general mechanism for size control. DOI: http://dx.doi.org/10.7554/eLife.10767.001 PMID:27015111

Audit Oversight: Quality Control System at U.S. Special Operations Command Inspector General Audit Division

DTIC Science & Technology

2002-08-21

The Audit Division provides the Commander, U.S. Special Operations Command (USSOCOM) with professional auditing services to safeguard, account for...and ensure the proper use of special operations forces assets in accomplishing the USSOCOM mission. The Audit Division reports to the USSOCOM Inspector...U.S. Army Special Operations Command, Naval Special Warfare Command, and the Joint Special Operations Command. Appendix A contains a summary of the Audit Division policy and procedures.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

This report contains the following information from the Instrumentation and Controls Division of Oak Ridge National Laboratory: supplementary activities; seminars; publications and presentations; scientific and professional activities, achievements, and awards; and division organization charts.

Responsibility for Teaching Pain Control in U.S. Dental Schools.

ERIC Educational Resources Information Center

Smith, Peter B.; Campbell, Robert L.

1993-01-01

A national survey of 53 dental schools found most were not interested in developing a separate division or department of dental anesthesiology. Of those with a dentist anesthesiologist responsible for teaching pain control, all have or favor such a division. Less than one-third employ professionals limiting their practice to anesthesiology. (MSE)

Instrumentation and Controls Division Progress Report for the Period of July 1, 1994 to December 31, 1997: Publications, Presentations, Activities, and Awards

DOE Office of Scientific and Technical Information (OSTI.GOV)

McDonald, D.W.

This report contains a record of publishing and other activities in the Oak Ridge National Laboratory (ORNL) Instrumentation and Controls (I&C) Division for the period of July 1, 1994, to December31, 1997. It is a companion volume to Working Together on New Horizons: Instrumentation and Controls Division Progress Report for the Period of July 1, 1994, to December 31, 1997 (OR.NLA4-6530). Working Together on New Horizons contains illustrated summaries of some of the projects under way in I&C Division. Both books can be obtained by contacting C. R. Brittain (brittain@ornl. gov), P.O. Box 2008, Oak Ridge, TN 37831-6005. l&C Divisionmore » Mission and Vision I&C Division develops and maintains techniques, instruments, and systems that lead to a better understanding of nature and harnessing of natural phenomena for the benefit of humankind. We have dedicated ourselves to accelerating the advancement of science and the transfer of those advancements into products and processes that benefit U.S. industry and enhance the security of our citizens.« less

Joy Osborne, MS, MPA | Division of Cancer Prevention

Cancer.gov

Joy Osborne is the ARC Director for the Division of Cancer Prevention and the Division of Cancer Control and Population Sciences. The ARC (Administrative Resource Center) provides services to DCP in the areas of budget, contracts, grants, human resources, travel, space and facilities, and other administrative areas. Joy came to NCI in 1992 as a Presidential Management Intern

Asymmetric cell division requires specific mechanisms for adjusting global transcription.

PubMed

Mena, Adriana; Medina, Daniel A; García-Martínez, José; Begley, Victoria; Singh, Abhyudai; Chávez, Sebastián; Muñoz-Centeno, Mari C; Pérez-Ortín, José E

2017-12-01

Most cells divide symmetrically into two approximately identical cells. There are many examples, however, of asymmetric cell division that can generate sibling cell size differences. Whereas physical asymmetric division mechanisms and cell fate consequences have been investigated, the specific problem caused by asymmetric division at the transcription level has not yet been addressed. In symmetrically dividing cells the nascent transcription rate increases in parallel to cell volume to compensate it by keeping the actual mRNA synthesis rate constant. This cannot apply to the yeast Saccharomyces cerevisiae, where this mechanism would provoke a never-ending increasing mRNA synthesis rate in smaller daughter cells. We show here that, contrarily to other eukaryotes with symmetric division, budding yeast keeps the nascent transcription rates of its RNA polymerases constant and increases mRNA stability. This control on RNA pol II-dependent transcription rate is obtained by controlling the cellular concentration of this enzyme. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

Cortical PAR polarity proteins promote robust cytokinesis during asymmetric cell division

PubMed Central

Jordan, Shawn N.; Davies, Tim; Zhuravlev, Yelena; Dumont, Julien; Shirasu-Hiza, Mimi

2016-01-01

Cytokinesis, the physical division of one cell into two, is thought to be fundamentally similar in most animal cell divisions and driven by the constriction of a contractile ring positioned and controlled solely by the mitotic spindle. During asymmetric cell divisions, the core polarity machinery (partitioning defective [PAR] proteins) controls the unequal inheritance of key cell fate determinants. Here, we show that in asymmetrically dividing Caenorhabditis elegans embryos, the cortical PAR proteins (including the small guanosine triphosphatase CDC-42) have an active role in regulating recruitment of a critical component of the contractile ring, filamentous actin (F-actin). We found that the cortical PAR proteins are required for the retention of anillin and septin in the anterior pole, which are cytokinesis proteins that our genetic data suggest act as inhibitors of F-actin at the contractile ring. Collectively, our results suggest that the cortical PAR proteins coordinate the establishment of cell polarity with the physical process of cytokinesis during asymmetric cell division to ensure the fidelity of daughter cell formation. PMID:26728855

Control of cell division in Streptococcus pneumoniae by the conserved Ser/Thr protein kinase StkP.

PubMed

Beilharz, Katrin; Nováková, Linda; Fadda, Daniela; Branny, Pavel; Massidda, Orietta; Veening, Jan-Willem

2012-04-10

How the human pathogen Streptococcus pneumoniae coordinates cell-wall synthesis during growth and division to achieve its characteristic oval shape is poorly understood. The conserved eukaryotic-type Ser/Thr kinase of S. pneumoniae, StkP, previously was reported to phosphorylate the cell-division protein DivIVA. Consistent with a role in cell division, GFP-StkP and its cognate phosphatase, GFP-PhpP, both localize to the division site. StkP localization depends on its penicillin-binding protein and Ser/Thr-associated domains that likely sense uncross-linked peptidoglycan, because StkP and PhpP delocalize in the presence of antibiotics that target the latest stages of cell-wall biosynthesis and in cells that have stopped dividing. Time-lapse microscopy shows that StkP displays an intermediate timing of recruitment to midcell: StkP arrives shortly after FtsA but before DivIVA. Furthermore, StkP remains at midcell longer than FtsA, until division is complete. Cells mutated for stkP are perturbed in cell-wall synthesis and display elongated morphologies with multiple, often unconstricted, FtsA and DivIVA rings. The data show that StkP plays an important role in regulating cell-wall synthesis and controls correct septum progression and closure. Overall, our results indicate that StkP signals information about the cell-wall status to key cell-division proteins and in this way acts as a regulator of cell division.

A Thermal Management System Using Ammonium Carbamate as an Endothermic Heat Sink (POSTPRINT)

DTIC Science & Technology

2017-04-01

Niedbalski and Soumya S. Patnaik Mechanical and Thermal Systems Branch Power and Control Division Douglas J. Johnson and Jamie S. Ervin University...failing to comply with a collection of information if it does not display a currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE...and Control Division Air Force Research Laboratory, Aerospace Systems Directorate Wright-Patterson Air Force Base, OH 45433-7542 Air Force Materiel

40Gbit/s MDM-WDM Laguerre-Gaussian Mode with Equalization for Multimode Fiber in Access Networks

NASA Astrophysics Data System (ADS)

Fazea, Yousef; Amphawan, Angela

2018-04-01

Modal dispersion is seen as the primary impairment for multimode fiber. Mode division multiplexing (MDM) is a promising technology that has been realized as a favorable technology for considerably upsurges the capacity and distance of multimode fiber in conjunction with Wavelength Division Multiplexing (WDM) for fiber-to-the-home. This paper reveals the importance of an equalization technique in conjunction with controlling the modes spacing of mode division multiplexing-wavelength division multiplexing of Laguerre-Gaussian modes to alleviate modal dispersion for multimode fiber. The effects of channel spacing of 20 channels MDM-WDM were examined through controlling the azimuthal mode number and the radial mode number of Laguerre-Gaussian modes. A data rate of 40Gbit/s was achieved for a distance of 1,500 m for MDM-WDM.

International Federation of Library Associations Annual Conference Papers. Bibliographic Control Division: Bibliography and Cataloguing Sections (47th, Leipzig, East Germany, August 17-22, 1981).

ERIC Educational Resources Information Center

Vita, Susan H.; And Others

This set of papers delivered to the Bibliographic Control Division of the International Federation of Library Associations (IFLA) during its 47th annual conference (1981) includes: "Cataloging in Publication in the United States--Problems and Prospects," by Susan H. Vita; "Development and Coordination of Bibliographic Activities:…

Chromosome segregation drives division site selection in Streptococcus pneumoniae.

PubMed

van Raaphorst, Renske; Kjos, Morten; Veening, Jan-Willem

2017-07-18

Accurate spatial and temporal positioning of the tubulin-like protein FtsZ is key for proper bacterial cell division. Streptococcus pneumoniae (pneumococcus) is an oval-shaped, symmetrically dividing opportunistic human pathogen lacking the canonical systems for division site control (nucleoid occlusion and the Min-system). Recently, the early division protein MapZ was identified and implicated in pneumococcal division site selection. We show that MapZ is important for proper division plane selection; thus, the question remains as to what drives pneumococcal division site selection. By mapping the cell cycle in detail, we show that directly after replication both chromosomal origin regions localize to the future cell division sites, before FtsZ. Interestingly, Z-ring formation occurs coincidently with initiation of DNA replication. Perturbing the longitudinal chromosomal organization by mutating the condensin SMC, by CRISPR/Cas9-mediated chromosome cutting, or by poisoning DNA decatenation resulted in mistiming of MapZ and FtsZ positioning and subsequent cell elongation. Together, we demonstrate an intimate relationship between DNA replication, chromosome segregation, and division site selection in the pneumococcus, providing a simple way to ensure equally sized daughter cells.

User Guide for Unmanned Aerial System (UAS) Operations on the National Ranges

DTIC Science & Technology

2007-11-01

WARFARE CENTER WEAPONS DIVISION, PT. MUGU NAVAL AIR WARFARE CENTER WEAPONS DIVISION, CHINA LAKE NAVAL AIR WARFARE CENTER AIRCRAFT DIVISION, PATUXENT...with IFR Instrument Flight Rules MRTFB Major Range and Test Facility Base NAS National Airspace System NM nautical mile NTIA National...sectional charts, Instrument Flight Rules ( IFR ) enroute charts, and terminal area charts. The floor and ceiling, operating hours, and controlling

Aligned Carbon Nanotube Carpets on Carbon Substrates for High Power Electronic Applications

DTIC Science & Technology

2016-06-01

SiOx by a vapor-solid-solid mechanism ,” J. Am. Chem. Soc., vol. 133, pp. 197–199, 2011. [146] B. Liu, W. Ren, C. Liu, C.-H. Sun , L. Gao, S. Li, C... Mechanical and Thermal Systems Branch Power and Control Division JUNE 2016 Interim Report DISTRIBUTION STATEMENT A: Approved for public release...Advisor Program Engineer Mechanical and Thermal Systems Branch Mechanical and Thermal Systems Branch Power and Control Division Power and Control

Cytoplasmic removal, enucleation, and cell fusion of mouse oocytes.

PubMed

Kyogoku, Hirohisa; Yoshida, Shuhei; Kitajima, Tomoya S

2018-01-01

Meiotic divisions in females occur in fully grown oocytes that have a large cytoplasmic volume. The intracellular processes that are needed to accomplish meiotic divisions, such as spindle formation, chromosome segregation, and polar body extrusion, are controlled by the concerted actions of nuclear and cytoplasmic factors, which exhibit dynamic quantitative and spatiotemporal changes during meiotic maturation. Thus, distinguishing between meiotic controls that are mediated by cytoplasmic factors and those mediated by nuclear factors helps in the understanding of the mechanisms underlying meiotic divisions. Here, we describe a method to artificially modify the number of nuclei and the volume of the cytoplasm of mouse oocytes through cytoplasmic removal, enucleation, and cell fusion. The oocytes generated by this method are viable and undergo reproducible meiotic divisions exhibiting the effects of altered amounts of cytoplasmic and nuclear factors, which can be analyzed by various assays, such as live imaging microscopy. © 2018 Elsevier Inc. All rights reserved.

Advanced Aircraft Electrical System Control Technology Demonstrator. Phase I. Requirements Analysis and Conceptual Design.

DTIC Science & Technology

1981-07-01

System 13 (7) Flight Critical Power 15 (8) Power Bus Configuration 16 b. System Control and Protection 20...includes the main buses, external power receptacles and distribution feeders. The function of the distribution protection system * is mainly to provide...TechnicaI rea Manager Power Systems Branch Power Systems B nch Aerospace Power Division Aerospace Power Division FOR .AKE D . REAMS Chief,

INSTRUMENTATION AND CONTROLS DIVISION, ELECTRICAL DESIGN STANDARDS AND GRAPHICAL SYMBOLS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bates, A.E.G.; Bowelle, M.M.; Horton, J.L.

1960-10-01

Recommendations of the Instrumentation and Controls Division Committee on Electrical and Electronic Symbols and Drawings are presented. The American Standards Associrtion Graphical Symbols for Electrical Diagrams are given, with certain variations or additions recommended by the Committee to clarify or more positively identify the device or element symbolized. Recommendations regarding electrical elementary diagram 1ayout, device coding, etc., are included. (W.D.M.)

IFLA General Conference, 1990. Division of Bibliographic Control: Open Forum; Section of Cataloguing; Section of Bibliography; Section of Classification and Indexing. Booklet 4.

ERIC Educational Resources Information Center

International Federation of Library Associations, The Hague (Netherlands).

The 10 papers in this collection were presented at an open forum and meetings of three sections within the Division of Bibliographic Control: (1) "Review of the Work of the Section on Cataloguing 1989/1990" (Nancy R. John and Inger Cathrine Spangen); (2) "Report from the Section on Classification and Indexing" (Dorothy…

Annual Report, Reservoir Control Center, Southwestern Division (1989)

DTIC Science & Technology

1990-01-01

Division in the water quality field . This provides for water quality objectives being included as an effective part of our total water management...WES) selected Canyon Lake as a research field site for developing techniques to evaluate the impacts associated with installation of hydropower at Corps...term continuous goals of this Division, and consequently the Water Management Branch, in the water quality field . (1) To obtain sufficient water

Distributed Processing Tools Definition. Volume 1. Hardware and Software Technologies for Tightly-Coupled Distributed Systems.

DTIC Science & Technology

1983-06-01

LOSARDO Project Engineer APPROVED: .MARMCINIhI, Colonel. USAF Chief, Coaud and Control Division FOR THE CCOaIDKR: Acting Chief, Plea Off ice * **711...WORK UNIT NUMBERS General Dynamics Corporation 62702F Data Systems Division P 0 Box 748, Fort Worth TX 76101 55811829 I1. CONTROLLING OFFICE NAME AND...Processing System for 29 the Operation/Direction Center(s) 4-3 Distribution of Processing Control 30 for the Operation/Direction Center(s) 4-4 Generalized

Control of Anion in Corporation in the Molecular Beam Epitaxy of Ternary Antimonide Superlattices for Very Long Wavelength Infrared Detection (Postprint)

DTIC Science & Technology

2015-10-01

ASSIGNED DISTRIBUTION STATEMENT. //Signature// //Signature// GAIL J. BROWN DIANA M. CARLIN, Chief Nanoelectronic ...Materials Branch Nanoelectronic Materials Branch Functional Materials Division Functional Materials Division //Signature// KAREN

BASL and EPF2 act independently to regulate asymmetric divisions during stomatal development

PubMed Central

Hunt, Lee

2010-01-01

The initiation of stomatal development in the developing Arabidopsis epidermis is characterized by an asymmetric ‘entry’ division in which a small cell, known as a meristemoid, and a larger daughter cell is formed. The meristemoid may undergo further asymmetric divisions, regenerating a meristemoid each time, before differentiating into a guard mother cell which divides symmetrically to form a pair of guard cells surrounding a stomatal pore. Recently EPF2 and BASL have emerged as regulators of these asymmetric divisions and here we present results indicating that these two factors operate independently to control stomatal development PMID:20220310

CedA is a novel Escherichia coli protein that activates the cell division inhibited by chromosomal DNA over-replication.

PubMed

Katayama, T; Takata, M; Sekimizu, K

1997-11-01

We isolated and characterized a new gene related to the control of cell division regulation in Escherichia coli. At 30 degrees C, the dnaAcos mutant causes over-replication of the chromosome, and colony formation is inhibited. We found that, at this temperature, the dnaAcos cells form filaments; therefore, septum formation is inhibited. This inhibition was independent of SfiA, an inhibitor of the septum-forming protein, FtsZ. To identify factors involved in this pathway of inhibition, we isolated seven multicopy suppressors for the cold-sensitive phenotype of the dnaAcos mutant. One of these proved to be a previously unknown gene, which we named cedA. This gene encoded a 12 kDa protein and resided at 38.9min on the E. coli genome map. A multicopy supply of the cedA gene to the dnaAcos cells did not repress over-replication of the chromosome but did stimulate cell division of the host, the result being growth of cells with an abnormally elevated chromosomal copy number. Therefore, the expression level of the cedA gene seems to be important for inhibiting cell division of the dnaAcos mutant at 30 degrees C. We propose that over-replication of the chromosome activates a pathway for inhibiting cell division and that the cedA gene modulates this division control. In the dnaA+ background, cedA also seems to affect cell division.

Mechanical Forces Program the Orientation of Cell Division during Airway Tube Morphogenesis.

PubMed

Tang, Zan; Hu, Yucheng; Wang, Zheng; Jiang, Kewu; Zhan, Cheng; Marshall, Wallace F; Tang, Nan

2018-02-05

Oriented cell division plays a key role in controlling organogenesis. The mechanisms for regulating division orientation at the whole-organ level are only starting to become understood. By combining 3D time-lapse imaging, mouse genetics, and mathematical modeling, we find that global orientation of cell division is the result of a combination of two types of spindles with distinct spindle dynamic behaviors in the developing airway epithelium. Fixed spindles follow the classic long-axis rule and establish their division orientation before metaphase. In contrast, rotating spindles do not strictly follow the long-axis rule and determine their division orientation during metaphase. By using both a cell-based mechanical model and stretching-lung-explant experiments, we showed that mechanical force can function as a regulatory signal in maintaining the stable ratio between fixed spindles and rotating spindles. Our findings demonstrate that mechanical forces, cell geometry, and oriented cell division function together in a highly coordinated manner to ensure normal airway tube morphogenesis. Copyright © 2017 Elsevier Inc. All rights reserved.

Division by zero, pseudo-division by zero, Zhang dynamics method and Zhang-gradient method about control singularity conquering

NASA Astrophysics Data System (ADS)

Zhang, Yunong; Zhang, Yinyan; Chen, Dechao; Xiao, Zhengli; Yan, Xiaogang

2017-01-01

In this paper, the division-by-zero (DBO) problem in the field of nonlinear control, which is traditionally termed the control singularity problem (or specifically, controller singularity problem), is investigated by the Zhang dynamics (ZD) method and the Zhang-gradient (ZG) method. According to the impact of the DBO problem on the state variables of the controlled nonlinear system, the concepts of the pseudo-DBO problem and the true-DBO problem are proposed in this paper, which provide a new perspective for the researchers on the DBO problems as well as nonlinear control systems. Besides, the two classes of DBO problems are solved under the framework of the ZG method. Specific examples are shown and investigated in this paper to illustrate the two proposed concepts and the efficacy of the ZG method in conquering pseudo-DBO and true-DBO problems. The application of the ZG method to the tracking control of a two-wheeled mobile robot further substantiates the effectiveness of the ZG method. In addition, the ZG method is successfully applied to the tracking control of a pure-feedback nonlinear system.

Fires, A Joint Professional Bulletin for US Field & Air Defense Artillerymen. March-April 2008

DTIC Science & Technology

2008-04-01

actions. However, if the effects synchronization division were not added to the lineup , just the teaming of joint fires division and IO division still...ADAFCO would be the liaison between the two Services who coordinates fires and facilitates track identification thus preventing fratricide. Joint...decisions to be made based on current air users versus planned control measures. Interoperability. Identification is a key feature that enables AC2 nodes

The Science of Cancer Prevention

Cancer.gov

The science of cancer prevention is described by Dr. Barnett S. Kramer, M.D., M.P.H., director of the Division of Cancer Prevention, National Cancer Institute (NCI). The Division of Cancer Prevention administers a broad spectrum of research that spans basic pre-clinical, laboratory research, supportive and palliative care research, early detection, and randomized controlled clinical trials. The Division also supports the Cancer Prevention Fellowship Program and is devoted to the balanced communication of scientific results.

Interdependency of formation and localisation of the Min complex controls symmetric plastid division.

PubMed

Maple, Jodi; Møller, Simon G

2007-10-01

Plastid division represents a fundamental biological process essential for plant development; however, the molecular basis of symmetric plastid division is unclear. AtMinE1 plays a pivotal role in selection of the plastid division site in concert with AtMinD1. AtMinE1 localises to discrete foci in chloroplasts and interacts with AtMinD1, which shows a similar localisation pattern. Here, we investigate the importance of Min protein complex formation during the chloroplast division process. Dissection of the assembly of the Min protein complex and determination of the interdependency of complex assembly and localisation in planta allow us to present a model of the molecular basis of selection of the division site in plastids. Moreover, functional analysis of AtMinE1 in bacteria demonstrates the level of functional conservation and divergence of the plastidic MinE proteins.

Myo19 ensures symmetric partitioning of mitochondria and coupling of mitochondrial segregation to cell division.

PubMed

Rohn, Jennifer L; Patel, Jigna V; Neumann, Beate; Bulkescher, Jutta; Mchedlishvili, Nunu; McMullan, Rachel C; Quintero, Omar A; Ellenberg, Jan; Baum, Buzz

2014-11-03

During animal cell division, an actin-based ring cleaves the cell into two. Problems with this process can cause chromosome missegregation and defects in cytoplasmic inheritance and the partitioning of organelles, which in turn are associated with human diseases. Although much is known about how chromosome segregation is coupled to cell division, the way organelles coordinate their inheritance during partitioning to daughter cells is less well understood. Here, using a high-content live-imaging small interfering RNA screen, we identify Myosin-XIX (Myo19) as a novel regulator of cell division. Previously, this actin-based motor was shown to control the interphase movement of mitochondria. Our analysis shows that Myo19 is indeed localized to mitochondria and that its silencing leads to defects in the distribution of mitochondria within cells and in mitochondrial partitioning at division. Furthermore, many Myo19 RNAi cells undergo stochastic division failure--a phenotype that can be mimicked using a treatment that blocks mitochondrial fission and rescued by decreasing mitochondrial fusion, implying that mitochondria can physically interfere with cytokinesis. Strikingly, using live imaging we also observe the inappropriate movement of mitochondria to the poles of spindles in cells depleted for Myo19 as they enter anaphase. Since this phenocopies the results of an acute loss of actin filaments in anaphase, these data support a model whereby the Myo19 actin-based motor helps to control mitochondrial movement to ensure their faithful segregation during division. The presence of DNA within mitochondria makes their inheritance an especially important aspect of symmetrical cell division. Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.

New Mission Control Center Briefing

NASA Technical Reports Server (NTRS)

1995-01-01

Live footage shows panelists, Chief Center Systems Division John Muratore, and Acting Chief, Control Center Systems Division, Linda Uljon, giving an overview of the new Mission Control Center. Muratore and Uljon talk about the changes and modernization of the new Center. The panelists mention all the new capabilities of the new Center. They emphasize the Distributed real time command and control environment, the reduction in operation costs, and even the change from coaxial cables to fiber optic cables. Uljon also tells us that the new Control Center will experience its first mission after the launch of STS-70 and its first complete mission (both launching and landing) during STS-71.

The Nematode Caenorhabditis Elegans.

ERIC Educational Resources Information Center

Kenyon, Cynthia

1988-01-01

Discusses advantages of nematode use for studying patterns of cell division, differentiation, and morphogenesis. Describes nematode development. Cites experimental approaches available for genetic studies. Reviews the topics of control of cell division and differentiation, the nervous system, and muscle assembly and function of the organism. (RT)

Sexual Violence Prevention

MedlinePlus

... Intimate Partner and Sexual Violence Survey National Sexual Violence Resource Center Features Media Sign up for Features Get Email Updates To ... Center for Injury Prevention and Control, Division of Violence Prevention Page maintained by: Office of the Associate Director for Communication, Digital Media Branch, Division of Public Affairs ... ...

SSD1, which encodes a plant-specific novel protein, controls plant elongation by regulating cell division in rice.

PubMed

Asano, Kenji; Miyao, Akio; Hirochika, Hirohiko; Kitano, Hidemi; Matsuoka, Makoto; Ashikari, Motoyuki

2010-01-01

Plant height is one of the most important traits in crop improvement. Therefore revealing the mechanism of plant elongation and controlling plant height in accordance with breeding object is important. In this study we analyzed a novel dwarf mutant, ssd1, of which phenotype is different from typical GA- or BR-related dwarf phenotype. ssd1 exhibits pleiotropic defects in elongation of various organs such as stems, roots, leaves, and flowers. ssd1 also shows abnormal cell files and shapes, which suggests defects of normal cell division in the mutant. Map-based cloning and complementation test demonstrated that the dwarf phenotype in ssd1 mutant was caused by insertion of retrotransposon in a gene, which encodes plant-specific protein with unknown biochemical function. A BLAST search revealed that SSD1-like genes exist in diverse plant species, including monocots and dicots, but not fern and moss. Our results demonstrate that SSD1 controls plant elongation by controlling cell division in higher plants.

TfVPS32 Regulates Cell Division in the Parasite Tritrichomonas foetus.

PubMed

Iriarte, Lucrecia S; Midlej, Victor; Frontera, Lorena S; Moros Duarte, Daniel; Barbeito, Claudio G; de Souza, Wanderley; Benchimol, Marlene; de Miguel, Natalia; Coceres, Veronica M

2018-01-01

The flagellated protist Tritrichomonas foetus is a parasite that causes bovine trichomonosis, a major sexually transmitted disease in cattle. Cell division has been described as a key player in controlling cell survival in other cells, including parasites but there is no information on the regulation of this process in T. foetus. The regulation of cytokinetic abscission, the final stage of cell division, is mediated by members of the ESCRT (endosomal sorting complex required for transport) machinery. VPS32 is a subunit within the ESCRTIII complex and here, we report that TfVPS32 is localized on cytoplasmic vesicles and a redistribution of the protein to the midbody is observed during the cellular division. In concordance with its localization, deletion of TfVPS32 C-terminal alpha helices (α5 helix and/or α4-5 helix) leads to abnormal T. foetus growth, an increase in the percentage of multinucleated parasites and cell cycle arrest at G2/M phase. Together, these results indicate a role of this protein in controlling normal cell division. © 2017 The Author(s) Journal of Eukaryotic Microbiology © 2017 International Society of Protistologists.

Accurate Cell Division in Bacteria: How Does a Bacterium Know Where its Middle Is?

NASA Astrophysics Data System (ADS)

Howard, Martin; Rutenberg, Andrew

2004-03-01

I will discuss the physical principles lying behind the acquisition of accurate positional information in bacteria. A good application of these ideas is to the rod-shaped bacterium E. coli which divides precisely at its cellular midplane. This positioning is controlled by the Min system of proteins. These proteins coherently oscillate from end to end of the bacterium. I will present a reaction-diffusion model that describes the diffusion of the Min proteins, and their binding/unbinding from the cell membrane. The system possesses an instability that spontaneously generates the Min oscillations, which control accurate placement of the midcell division site. I will then discuss the role of fluctuations in protein dynamics, and investigate whether fluctuations set optimal protein concentration levels. Finally I will examine cell division in a different bacteria, B. subtilis. where different physical principles are used to regulate accurate cell division. See: Howard, Rutenberg, de Vet: Dynamic compartmentalization of bacteria: accurate division in E. coli. Phys. Rev. Lett. 87 278102 (2001). Howard, Rutenberg: Pattern formation inside bacteria: fluctuations due to the low copy number of proteins. Phys. Rev. Lett. 90 128102 (2003). Howard: A mechanism for polar protein localization in bacteria. J. Mol. Biol. 335 655-663 (2004).

Coaxial Measurement of the Translational Distribution of CS Produced in the Laser Photolysis of CS2 at 193nm.

DTIC Science & Technology

1983-11-04

Division AREA & WORK UNIT NUMBERS . Department of Chemistry Howard University Washington, D. C. 20059 NR-051-733 1t. CONTROLLING OFFICE NAME AND...Journal of Physical Chemistry Laser Chemistry Division Department of Chemistry Howard University Washington, D. C. 20059 November 4, 1983 *Reproduction in...Victor McCrary, David Zakheim, and William M. Jackson Laser Chemistry Division Chemistry Departmient Howard University Washington, D.C.. 20059 ABSTRACT The

[Right extremities pain caused by a malacia lesion in the left putamen:a resting functional magnetic resonance imaging of the marginal division of the human brain].

PubMed

Chen, Zhi-Ye; Ma, Lin

2014-04-01

To explore the role of marginal division of the human brain in the pain modulation. Resting functional magnetic resonance imaging was applied in a patient with right extremities pain caused by a malacia lesion in the left putamen and in 8 healthy volunteers. Marginal division was defined using manual drawing on structure images, and was applied to the computation of fuctional connectivity maps. The functional connectivities in the left marginal division showed an evident decrease in the patient when compared with healthy controls. These connectivities were mainly located in the bilateral head of caudate nucleus, putamen, and left globus pallidus. The marginal division may be involved in the pain modulation.

Asymmetric cell division during T cell development controls downstream fate

PubMed Central

Pham, Kim; Shimoni, Raz; Charnley, Mirren; Ludford-Menting, Mandy J.; Hawkins, Edwin D.; Ramsbottom, Kelly; Oliaro, Jane; Izon, David; Ting, Stephen B.; Reynolds, Joseph; Lythe, Grant; Molina-Paris, Carmen; Melichar, Heather; Robey, Ellen; Humbert, Patrick O.; Gu, Min

2015-01-01

During mammalian T cell development, the requirement for expansion of many individual T cell clones, rather than merely expansion of the entire T cell population, suggests a possible role for asymmetric cell division (ACD). We show that ACD of developing T cells controls cell fate through differential inheritance of cell fate determinants Numb and α-Adaptin. ACD occurs specifically during the β-selection stage of T cell development, and subsequent divisions are predominantly symmetric. ACD is controlled by interaction with stromal cells and chemokine receptor signaling and uses a conserved network of polarity regulators. The disruption of polarity by deletion of the polarity regulator, Scribble, or the altered inheritance of fate determinants impacts subsequent fate decisions to influence the numbers of DN4 cells arising after the β-selection checkpoint. These findings indicate that ACD enables the thymic microenvironment to orchestrate fate decisions related to differentiation and self-renewal. PMID:26370500

Real-time time-division color electroholography using a single GPU and a USB module for synchronizing reference light.

PubMed

Araki, Hiromitsu; Takada, Naoki; Niwase, Hiroaki; Ikawa, Shohei; Fujiwara, Masato; Nakayama, Hirotaka; Kakue, Takashi; Shimobaba, Tomoyoshi; Ito, Tomoyoshi

2015-12-01

We propose real-time time-division color electroholography using a single graphics processing unit (GPU) and a simple synchronization system of reference light. To facilitate real-time time-division color electroholography, we developed a light emitting diode (LED) controller with a universal serial bus (USB) module and the drive circuit for reference light. A one-chip RGB LED connected to a personal computer via an LED controller was used as the reference light. A single GPU calculates three computer-generated holograms (CGHs) suitable for red, green, and blue colors in each frame of a three-dimensional (3D) movie. After CGH calculation using a single GPU, the CPU can synchronize the CGH display with the color switching of the one-chip RGB LED via the LED controller. Consequently, we succeeded in real-time time-division color electroholography for a 3D object consisting of around 1000 points per color when an NVIDIA GeForce GTX TITAN was used as the GPU. Furthermore, we implemented the proposed method in various GPUs. The experimental results showed that the proposed method was effective for various GPUs.

Two Forkhead transcription factors regulate the division of cardiac progenitor cells by a Polo-dependent pathway

PubMed Central

Ahmad, Shaad M.; Tansey, Terese R.; Busser, Brian W.; Nolte, Michael T.; Jeffries, Neal; Gisselbrecht, Stephen S.; Rusan, Nasser M.; Michelson, Alan M.

2012-01-01

SUMMARY The development of a complex organ requires the specification of appropriate numbers of each of its constituent cell types, as well as their proper differentiation and correct positioning relative to each other. During Drosophila cardiogenesis, all three of these processes are controlled by jumeau (jumu) and Checkpoint suppressor homologue (CHES-1-like), two genes encoding forkhead transcription factors that we discovered utilizing an integrated genetic, genomic and computational strategy for identifying genes expressed in the developing Drosophila heart. Both jumu and CHES-1-like are required during asymmetric cell division for the derivation of two distinct cardiac cell types from their mutual precursor, and in symmetric cell divisions that produce yet a third type of heart cell. jumu and CHES-1-like control the division of cardiac progenitors by regulating the activity of Polo, a kinase involved in multiple steps of mitosis. This pathway demonstrates how transcription factors integrate diverse developmental processes during organogenesis. PMID:22814603

A Small RNA-Catalytic Argonaute Pathway Tunes Germline Transcript Levels to Ensure Embryonic Divisions

PubMed Central

Gerson-Gurwitz, Adina; Wang, Shaohe; Sathe, Shashank; Green, Rebecca; Yeo, Gene W.; Oegema, Karen; Desai, Arshad

2016-01-01

SUMMARY Multiple division cycles without growth are a characteristic feature of early embryogenesis. The female germline loads proteins and RNAs into oocytes to support these divisions, which lack many quality control mechanisms operating in somatic cells undergoing growth. Here we describe a small RNA-Argonaute pathway that ensures early embryonic divisions in C. elegans by employing catalytic slicing activity to broadly tune, instead of silence, germline gene expression. Misregulation of one target, a kinesin-13 microtubule depolymerase, underlies a major phenotype associated with pathway loss. Tuning of target transcript levels is guided by density of homologous small RNAs, whose generation must ultimately be related to target sequence. Thus, the tuning action of a small RNA-catalytic Argonaute pathway generates oocytes capable of supporting embryogenesis. We speculate that the specialized nature of germline chromatin led to emergence of small RNA-catalytic Argonaute pathways in the female germline as a post-transcriptional control layer to optimize oocyte composition. PMID:27020753

Problems and potentialities of cultured plant cells in retrospect and prospect

NASA Technical Reports Server (NTRS)

Steward, F. C.; Krikorian, A. D.

1979-01-01

The past, present and expected future accomplishments and limitations of plant cell and tissue culture are reviewed. Consideration is given to the pioneering insights of Haberlandt in 1902, the development of culture techniques, and past work on cell division, cell and tissue growth and development, somatic embryogenesis, and metabolism and respiration. Current activity in culture media and technique development for plant regions, organs, tissues, cells, protoplasts, organelles and embryos, totipotency, somatic embryogenesis and clonal propagation under normal and space conditions, biochemical potentialities, and genetic engineering is surveyed. Prospects for the investigation of the induced control of somatic cell division, the division of isolated protoplasts, the improvement of haploid cell cultures, liquid cultures for somatic embryogenesis, and the genetic control of development are outlined.

Occludin Independently Regulates Permeability under Hydrostatic Pressure and Cell Division in Retinal Pigment Epithelial Cells

PubMed Central

Phillips, Brett E.; Cancel, Limary; Tarbell, John M.; Antonetti, David A.

2008-01-01

Purpose The aim of this study was to determine the function of the tight junction protein occludin in the control of permeability, under diffusive and hydrostatic pressures, and its contribution to the control of cell division in retinal pigment epithelium. Methods Occludin expression was inhibited in the human retinal pigment epithelial cell line ARPE-19 by siRNA. Depletion of occludin was confirmed by Western blot, confocal microscopy, and RT-PCR. Paracellular permeability of cell monolayers to fluorescently labeled 70 kDa dextran, 10 kDa dextran, and 467 Da tetramethylrhodamine (TAMRA) was examined under diffusive conditions or after the application of 10 cm H2O transmural pressure. Cell division rates were determined by tritiated thymidine incorporation and Ki67 immunoreactivity. Cell cycle inhibitors were used to determine whether changes in cell division affected permeability. Results Occludin depletion increased diffusive paracellular permeability to 467 Da TAMRA by 15%, and permeability under hydrostatic pressure was increased 50% compared with control. Conversely, depletion of occludin protein with siRNA did not alter diffusive permeability to 70 kDa and 10 kDa RITC-dextran, and permeability to 70 kDa dextran was twofold lower in occludin-depleted cells under hydrostatic pressure conditions. Occludin depletion also increased thymidine incorporation by 90% and Ki67-positive cells by 50%. Finally, cell cycle inhibitors did not alter the effect of occludin siRNA on paracellular permeability. Conclusions The data suggest that occludin regulates tight junction permeability in response to changes in hydrostatic pressure. Furthermore, these data suggest that occludin also contributes to the control of cell division, demonstrating a novel function for this tight junction protein. PMID:18263810

National Program for Inspection of Non-Federal Dams. Collins Company Upper Dam (CT 00674), Connecticut River Basin, Canton, Connecticut. Phase I Inspection Report.

DTIC Science & Technology

1979-07-01

Engineering Division p 0 CAR WE H FRZIAN, NENBER Design Branch Engineering Division J SEPE FIN~EGAN, JR.,CIV ater Control Branch * Engineering Division...Operator g. Purpose of Dam h. Design and Construction History i. Normal Operational Procedures 1.3 PERTINENT DATA ........................... 4 a...Tunnel i. Spillways j. Regulating Outlets SECTION 2: ENGINEERING DATA 2.1 DESIGN .............................. 9 a. Available Data b. Design Features c

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2014-10-01

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Code of Federal Regulations, 2010 CFR

2010-01-01

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Cell-Division Behavior in a Heterogeneous Swarm Environment.

PubMed

Erskine, Adam; Herrmann, J Michael

2015-01-01

We present a system of virtual particles that interact using simple kinetic rules. It is known that heterogeneous mixtures of particles can produce particularly interesting behaviors. Here we present a two-species three-dimensional swarm in which a behavior emerges that resembles cell division. We show that the dividing behavior exists across a narrow but finite band of parameters and for a wide range of population sizes. When executed in a two-dimensional environment the swarm's characteristics and dynamism manifest differently. In further experiments we show that repeated divisions can occur if the system is extended by a biased equilibrium process to control the split of populations. We propose that this repeated division behavior provides a simple model for cell-division mechanisms and is of interest for the formation of morphological structure and to swarm robotics.

Drosophila Sulf1 is required for the termination of intestinal stem cell division during regeneration.

PubMed

Takemura, Masahiko; Nakato, Hiroshi

2017-01-15

Stem cell division is activated to trigger regeneration in response to tissue damage. The molecular mechanisms by which this stem cell mitotic activity is properly repressed at the end of regeneration are poorly understood. Here, we show that a specific modification of heparan sulfate is crucial for regulating Drosophila intestinal stem cell (ISC) division during normal midgut homeostasis and regeneration. Loss of the extracellular heparan sulfate endosulfatase Sulf1 resulted in increased ISC division during normal homeostasis, which was caused by upregulation of mitogenic signaling including the JAK-STAT, EGFR and Hedgehog pathways. Using a regeneration model, we found that ISCs failed to properly halt division at the termination stage in Sulf1 mutants, showing that Sulf1 is required for terminating ISC division at the end of regeneration. We propose that post-transcriptional regulation of mitogen signaling by heparan sulfate structural modifications provides a new regulatory step for precise temporal control of stem cell activity during regeneration. © 2017. Published by The Company of Biologists Ltd.

Cyclin D regulation of a sexually dimorphic asymmetric cell division

PubMed Central

Tilmann, Christopher; Kimble, Judith

2006-01-01

SUMMARY The C. elegans somatic gonadal precursor cell (SGP) divides asymmetrically to establish gonad-specific coordinates in both sexes. In addition, the SGP division is sexually dimorphic and initiates sex-specific programs of gonadogenesis. Wnt/MAPK signaling determines the gonadal axes, and the FKH-6 transcription factor specifies the male mode of SGP division. In this paper, we demonstrate that C. elegans cyclin D controls POP-1/TCF asymmetry in the SGP daughters as well as fkh-6 and rnr expression in the SGPs. Although cyclin D mutants have delayed SGP divisions, the cyclin D defects are not mimicked by other methods of retarding the SGP division. We find that EFL-1/E2F has an antagonistic effect on fkh-6 expression and gonadogenesis, which is relieved by cyclin D activity. We propose that cyclin D and other canonical regulators of the G1/S transition coordinate key regulators of axis formation and sex determination with cell cycle progression to achieve the sexually dimorphic SGP asymmetric division. PMID:16198291

Drosophila Sulf1 is required for the termination of intestinal stem cell division during regeneration

PubMed Central

2017-01-01

ABSTRACT Stem cell division is activated to trigger regeneration in response to tissue damage. The molecular mechanisms by which this stem cell mitotic activity is properly repressed at the end of regeneration are poorly understood. Here, we show that a specific modification of heparan sulfate is crucial for regulating Drosophila intestinal stem cell (ISC) division during normal midgut homeostasis and regeneration. Loss of the extracellular heparan sulfate endosulfatase Sulf1 resulted in increased ISC division during normal homeostasis, which was caused by upregulation of mitogenic signaling including the JAK-STAT, EGFR and Hedgehog pathways. Using a regeneration model, we found that ISCs failed to properly halt division at the termination stage in Sulf1 mutants, showing that Sulf1 is required for terminating ISC division at the end of regeneration. We propose that post-transcriptional regulation of mitogen signaling by heparan sulfate structural modifications provides a new regulatory step for precise temporal control of stem cell activity during regeneration. PMID:27888216

High frame-rate resolution of cell division during Candida albicans filamentation

PubMed Central

Thomson, Darren D.; Berman, Judith; Brand, Alexandra C.

2016-01-01

The commensal yeast, Candida albicans, is an opportunistic pathogen in humans and forms filaments called hyphae and pseudohyphae, in which cell division requires precise temporal and spatial control to produce mononuclear cell compartments. High-frame-rate live-cell imaging (1 frame/min) revealed that nuclear division did not occur across the septal plane. We detected the presence of nucleolar fragments that may be extrachromosomal molecules carrying the ribosomal RNA genes. Cells occasionally maintained multiple nucleoli, suggesting either polyploidy, multiple nuclei and/or aneuploidy of ChrR., while the migration pattern of sister nuclei differed between unbranched and branched hyphae. The presented movie challenges and extends previous concepts of C. albicans cell division. PMID:26854071

75 FR 1024 - Proposed Significant New Use Rules on Certain Chemical Substances; Reopening of Comment Period

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2010-01-08

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Monitoring and Controlling Engineering and Construction Management Cost Performance Within the Corps of Engineers

DTIC Science & Technology

1988-12-01

COST MANAGEMENT The CMIF approach addresses total costs but does not permit the analysis of indirect costs. We found that indirect costs vary...responsibility USACE/divisions Increasing CMIF Districts/divisions level of by fund type detail G&A, technical indirect, burden Districts by fund type

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The case of the downsizing decision.

PubMed

Train, A S

1991-01-01

New CEO Charles Rampart's decision to make deep across-the-board cuts at Universal Products Company, Ltd. presents division manager Andrew Jordan with a thorny problem. Plagued by slow growth, a declining stock price, and an increasingly skeptical investment community, UPC needs to control costs and control them fast. But Jordan's division is the most profitable in the company, and the 11% cut proposed by Rampart could destroy already shaky morale and seriously threaten the division's ability to compete. "There comes a time in every manager's career when he has to fight a bad decision made by his boss," argues Sam Godwyn, Jordan's vice president for marketing and sales. "To cut across the board is to take a blunt axe to the company when a surgeon's scalpel is called for." He suggests it is better to line up support for an alternative plan that links cuts to a long-term strategy and that differentiates between successful and unsuccessful divisions. "It would be a terrible mistake for us to focus only on the narrow needs of the division when the future of the whole company is at stake," counters Mary Wyatt, Jordan's vice president for finance. Yes, the downsizing will hurt the division in the short term, but the real issue is getting behind the new CEO. Supporting the downsizing decision is a necessary investment in this future credibility and effectiveness--whatever the short-term costs. Four commentators debate Jordan's dilemma and how he should resolve it.

A local maximum in gibberellin levels regulates maize leaf growth by spatial control of cell division.

PubMed

Nelissen, Hilde; Rymen, Bart; Jikumaru, Yusuke; Demuynck, Kirin; Van Lijsebettens, Mieke; Kamiya, Yuji; Inzé, Dirk; Beemster, Gerrit T S

2012-07-10

Plant growth rate is largely determined by the transition between the successive phases of cell division and expansion. A key role for hormone signaling in determining this transition was inferred from genetic approaches and transcriptome analysis in the Arabidopsis root tip. We used the developmental gradient at the maize leaf base as a model to study this transition, because it allows a direct comparison between endogenous hormone concentrations and the transitions between dividing, expanding, and mature tissue. Concentrations of auxin and cytokinins are highest in dividing tissues, whereas bioactive gibberellins (GAs) show a peak at the transition zone between the division and expansion zone. Combined metabolic and transcriptomic profiling revealed that this GA maximum is established by GA biosynthesis in the division zone (DZ) and active GA catabolism at the onset of the expansion zone. Mutants defective in GA synthesis and signaling, and transgenic plants overproducing GAs, demonstrate that altering GA levels specifically affects the size of the DZ, resulting in proportional changes in organ growth rates. This work thereby provides a novel molecular mechanism for the regulation of the transition from cell division to expansion that controls organ growth and size. Copyright © 2012 Elsevier Ltd. All rights reserved.

Uhrf1 controls the self-renewal versus differentiation of hematopoietic stem cells by epigenetically regulating the cell-division modes

PubMed Central

Zhao, Jingyao; Chen, Xufeng; Song, Guangrong; Zhang, Jiali; Liu, Haifeng; Liu, Xiaolong

2017-01-01

Hematopoietic stem cells (HSCs) are able to both self-renew and differentiate. However, how individual HSC makes the decision between self-renewal and differentiation remains largely unknown. Here we report that ablation of the key epigenetic regulator Uhrf1 in the hematopoietic system depletes the HSC pool, leading to hematopoietic failure and lethality. Uhrf1-deficient HSCs display normal survival and proliferation, yet undergo erythroid-biased differentiation at the expense of self-renewal capacity. Notably, Uhrf1 is required for the establishment of DNA methylation patterns of erythroid-specific genes during HSC division. The expression of these genes is enhanced in the absence of Uhrf1, which disrupts the HSC-division modes by promoting the symmetric differentiation and suppressing the symmetric self-renewal. Moreover, overexpression of one of the up-regulated genes, Gata1, in HSCs is sufficient to phenocopy Uhrf1-deficient HSCs, which show impaired HSC symmetric self-renewal and increased differentiation commitment. Taken together, our findings suggest that Uhrf1 controls the self-renewal versus differentiation of HSC through epigenetically regulating the cell-division modes, thus providing unique insights into the relationship among Uhrf1-mediated DNA methylation, cell-division mode, and HSC fate decision. PMID:27956603

Mitosis-Specific Mechanosensing and Contractile Protein Redistribution Control Cell Shape

PubMed Central

Effler, Janet C.; Kee, Yee-Seir; Berk, Jason M.; Tran, Minhchau N.; Iglesias, Pablo A.; Robinson, Douglas N.

2008-01-01

Summary Because cell division failure is deleterious, promoting tumorigenesis in mammals [1], cells utilize numerous mechanisms to control their cell-cycle progression [2–4]. Though cell division is considered a well-ordered sequence of biochemical events [5], cytokinesis, an inherently mechanical process, must also be mechanically controlled to ensure that two equivalent daughter cells are produced with high fidelity. Since cells respond to their mechanical environment [6, 7], we hypothesized that cells utilize mechanosensing and mechanical feedback to sense and correct shape asymmetries during cytokinesis. Because the mitotic spindle and myosin-II are vital to cell division [8, 9], we explored their roles in responding to shape perturbations during cell division. We demonstrate that the contractile proteins, myosin-II and cortexillin-I, redistribute in response to intrinsic and externally induced shape asymmetries. In early cytokinesis, mechanical load overrides spindle cues and slows cytokinesis progression while contractile proteins accumulate and correct shape asymmetries. In late cytokinesis, mechanical perturbation also directs contractile proteins but without apparently disrupting cytokinesis. Significantly, this response only occurs during anaphase through cytokinesis, does not require microtubules, is independent of spindle orientation, but is dependent on myosin-II. Our data provide evidence for a mechanosensory system that directs contractile proteins to regulate cell shape during mitosis. PMID:17027494

An Autonomous Satellite Time Synchronization System Using Remotely Disciplined VC-OCXOs.

PubMed

Gu, Xiaobo; Chang, Qing; Glennon, Eamonn P; Xu, Baoda; Dempseter, Andrew G; Wang, Dun; Wu, Jiapeng

2015-07-23

An autonomous remote clock control system is proposed to provide time synchronization and frequency syntonization for satellite to satellite or ground to satellite time transfer, with the system comprising on-board voltage controlled oven controlled crystal oscillators (VC-OCXOs) that are disciplined to a remote master atomic clock or oscillator. The synchronization loop aims to provide autonomous operation over extended periods, be widely applicable to a variety of scenarios and robust. A new architecture comprising the use of frequency division duplex (FDD), synchronous time division (STDD) duplex and code division multiple access (CDMA) with a centralized topology is employed. This new design utilizes dual one-way ranging methods to precisely measure the clock error, adopts least square (LS) methods to predict the clock error and employs a third-order phase lock loop (PLL) to generate the voltage control signal. A general functional model for this system is proposed and the error sources and delays that affect the time synchronization are discussed. Related algorithms for estimating and correcting these errors are also proposed. The performance of the proposed system is simulated and guidance for selecting the clock is provided.

Coordination of size-control, reproduction and generational memory in freshwater planarians

NASA Astrophysics Data System (ADS)

Yang, Xingbo; Kaj, Kelson; Schwab, David; Collins, Eva-Maria

Uncovering the mechanisms that control size, growth, and division rates of systems reproducing through binary division means understanding basic principles of their life cycle. Recent work has focused on how division rates are regulated in bacteria and yeast, but this question has not yet been addressed in more complex, multicellular organisms. We have acquired a unique large-scale data set on the growth and asexual reproduction of two freshwater planarian species, Dugesia japonica and Dugesia tigrina, which reproduce by transverse fission and succeeding regeneration of head and tail pieces into new worms. We developed a new additive theoretical model that mixes multiple size control strategies based on worm size, growth, and waiting time. Our model quantifies the proportions of each strategy in the mixed dynamics, revealing the ability of the two planarian species to utilize different strategies in a coordinated manner for size control. Additionally, we found that head and tail offspring of both species employ different mechanisms to monitor and trigger their reproduction cycles. Finally, we show that generation-dependent memory effects in planarians need to be taken into account to accurately capture the experimental data.

Differences in cytokinin control on cellular dynamics of zucchini cotyledons cultivated in two experimental systems.

PubMed

Stoynova-Bakalova, E; Petrov, P; Gigova, L; Ivanova, N

2011-01-01

The effect of endogenous cytokinins on the pattern of palisade cell division post-germination does not depend on the conditions of cotyledon development -in planta (attached to seedlings) or in vitro (isolated from dry zucchini seeds and cultured on water). In cotyledons originating from 4-day-old seedlings (experimental system 1), exogenous cytokinin temporarily (in the first 2 day of cultivation) enhanced post-mitotic cell enlargement of palisade cells, mainly due to enhanced water uptake and use of cell storage compounds, all of which lead to cotyledon senescence. Cytokinin is not able to resume the completed palisade cell division on day 5. As a result, the number of cells and the final areas of treated and control cotyledons are quite similar. By contrast, the effects of cytokinin on cotyledons isolated from dry seeds (experimental system 2) are better expressed, promoting an increase in number of palisade cells accompanied by additional cotyledon area enlargement. However, the prolonged post-mitotic cell expansion in control cotyledons compensates for the reduced speed of cell growth and division activity and decreases differences in final cotyledon area between treatments. The results define cell division as the primary target of cytokinin stimulation in cotyledon tissues competent for division, and determine the temporal patterns of palisade cell cycling related to cotyledon age. This knowledge permits a better choice of experimental system to study effects on cell proliferation and cell growth, as well as cell enlargement and senescence-related events using physiologically homogeneous material. © 2010 German Botanical Society and The Royal Botanical Society of the Netherlands.

Multiple Duties for Spindle Assembly Checkpoint Kinases in Meiosis

PubMed Central

Marston, Adele L.; Wassmann, Katja

2017-01-01

Cell division in mitosis and meiosis is governed by evolutionary highly conserved protein kinases and phosphatases, controlling the timely execution of key events such as nuclear envelope breakdown, spindle assembly, chromosome attachment to the spindle and chromosome segregation, and cell cycle exit. In mitosis, the spindle assembly checkpoint (SAC) controls the proper attachment to and alignment of chromosomes on the spindle. The SAC detects errors and induces a cell cycle arrest in metaphase, preventing chromatid separation. Once all chromosomes are properly attached, the SAC-dependent arrest is relieved and chromatids separate evenly into daughter cells. The signaling cascade leading to checkpoint arrest depends on several protein kinases that are conserved from yeast to man. In meiosis, haploid cells containing new genetic combinations are generated from a diploid cell through two specialized cell divisions. Though apparently less robust, SAC control also exists in meiosis. Recently, it has emerged that SAC kinases have additional roles in executing accurate chromosome segregation during the meiotic divisions. Here, we summarize the main differences between mitotic and meiotic cell divisions, and explain why meiotic divisions pose special challenges for correct chromosome segregation. The less-known meiotic roles of the SAC kinases are described, with a focus on two model systems: yeast and mouse oocytes. The meiotic roles of the canonical checkpoint kinases Bub1, Mps1, the pseudokinase BubR1 (Mad3), and Aurora B and C (Ipl1) will be discussed. Insights into the molecular signaling pathways that bring about the special chromosome segregation pattern during meiosis will help us understand why human oocytes are so frequently aneuploid. PMID:29322045

Travel guidance system for vehicles

DOE Office of Scientific and Technical Information (OSTI.GOV)

Takanabe, K.; Yamamoto, M.; Ito, K.

1987-02-24

A travel guidance system is described for vehicles including: a heading sensor for detecting a direction of movement of a vehicle; a distance sensor for detecting a distance traveled by the vehicle; a map data storage medium preliminarily storing map data; a control unit for receiving a heading signal from the heading sensor and a distance signal from the distance sensor to successively compute a present position of the vehicle and for generating video signals corresponding to display data including map data from the map data storage medium and data of the present position; and a display having first andmore » second display portions and responsive to the video signals from the control unit to display on the first display portion a map and a present portion mark, in which: the map data storage medium comprises means for preliminarily storing administrative division name data and landmark data; and the control unit comprises: landmark display means for: (1) determining a landmark closest to the present position, (2) causing a position of the landmark to be displayed on the map and (3) retrieving a landmark massage concerning the landmark from the storage medium to cause the display to display the landmark message on the second display portion; division name display means for retrieving the name of an administrative division to which the present position belongs from the storage medium and causing the display to display a division name message on the second display portion; and selection means for selectively actuating at least one of the landmark display means and the division name display means.« less

Coordination of size-control, reproduction and generational memory in freshwater planarians

NASA Astrophysics Data System (ADS)

Yang, Xingbo; Kaj, Kelson J.; Schwab, David J.; Collins, Eva-Maria S.

2017-06-01

Uncovering the mechanisms that control size, growth, and division rates of organisms reproducing through binary division means understanding basic principles of their life cycle. Recent work has focused on how division rates are regulated in bacteria and yeast, but this question has not yet been addressed in more complex, multicellular organisms. We have, over the course of several years, assembled a unique large-scale data set on the growth and asexual reproduction of two freshwater planarian species, Dugesia japonica and Girardia tigrina, which reproduce by transverse fission and succeeding regeneration of head and tail pieces into new planarians. We show that generation-dependent memory effects in planarian reproduction need to be taken into account to accurately capture the experimental data. To achieve this, we developed a new additive model that mixes multiple size control strategies based on planarian size, growth, and time between divisions. Our model quantifies the proportions of each strategy in the mixed dynamics, revealing the ability of the two planarian species to utilize different strategies in a coordinated manner for size control. Additionally, we found that head and tail offspring of both species employ different mechanisms to monitor and trigger their reproduction cycles. Thus, we find a diversity of strategies not only between species but between heads and tails within species. Our additive model provides two advantages over existing 2D models that fit a multivariable splitting rate function to the data for size control: firstly, it can be fit to relatively small data sets and can thus be applied to systems where available data is limited. Secondly, it enables new biological insights because it explicitly shows the contributions of different size control strategies for each offspring type.

Coordination of size-control, reproduction and generational memory in freshwater planarians.

PubMed

Yang, Xingbo; Kaj, Kelson J; Schwab, David J; Collins, Eva-Maria S

2017-05-23

Uncovering the mechanisms that control size, growth, and division rates of organisms reproducing through binary division means understanding basic principles of their life cycle. Recent work has focused on how division rates are regulated in bacteria and yeast, but this question has not yet been addressed in more complex, multicellular organisms. We have, over the course of several years, assembled a unique large-scale data set on the growth and asexual reproduction of two freshwater planarian species, Dugesia japonica and Girardia tigrina, which reproduce by transverse fission and succeeding regeneration of head and tail pieces into new planarians. We show that generation-dependent memory effects in planarian reproduction need to be taken into account to accurately capture the experimental data. To achieve this, we developed a new additive model that mixes multiple size control strategies based on planarian size, growth, and time between divisions. Our model quantifies the proportions of each strategy in the mixed dynamics, revealing the ability of the two planarian species to utilize different strategies in a coordinated manner for size control. Additionally, we found that head and tail offspring of both species employ different mechanisms to monitor and trigger their reproduction cycles. Thus, we find a diversity of strategies not only between species but between heads and tails within species. Our additive model provides two advantages over existing 2D models that fit a multivariable splitting rate function to the data for size control: firstly, it can be fit to relatively small data sets and can thus be applied to systems where available data is limited. Secondly, it enables new biological insights because it explicitly shows the contributions of different size control strategies for each offspring type.

Cell cycles and cell division in the archaea.

PubMed

Samson, Rachel Y; Bell, Stephen D

2011-06-01

Until recently little was known about the cell cycle parameters and division mechanisms of archaeal organisms. Although this is still the case for the majority of archaea, significant advances have been made in some model species. The information that has been gleaned thus far points to a remarkable degree of diversity within the archaeal domain of life. More specifically, members of distinct phyla have very different chromosome copy numbers, replication control systems and even employ distinct machineries for cell division. Copyright © 2011 Elsevier Ltd. All rights reserved.

A History of the Acoustics Division of the Naval Research Laboratory: The First Eight Decades 1923 - 2008

DTIC Science & Technology

2013-08-01

Superintendent” goes back to the early days of NRL in the 1920s and has been retained to the present, refer- ring to the director of each NRL research division...installation, energy for the final amplifiers was stored essentially in the fuel tank of a gas- turbine -driven generator. Signifi- cant research in the Sound...Division found a method for control of the gas turbine which permitted it to supply power in long pulses without damage to the turbine . Research on

How PI3K-derived lipids control cell division.

PubMed

Campa, Carlo C; Martini, Miriam; De Santis, Maria C; Hirsch, Emilio

2015-01-01

To succeed in cell division, intense cytoskeletal and membrane remodeling are required to allow accurate chromosome segregation and cytoplasm partitioning. Spatial restriction of the actin dynamics and vesicle trafficking define the cell symmetry and equivalent membrane scission events, respectively. Protein complexes coordinating mitosis are recruited to membrane microdomains characterized by the presence of the phosphatidylinositol lipid members (PtdIns), like PtdIns(3,4,5)P 3,PtdIns(4,5)P 2, and PtdIns(3)P. These PtdIns represent a minor component of cell membranes, defining membrane domain identity, ultimately controlling cytoskeleton and membrane dynamics during mitosis. The coordinated presence of PtdIns(3,4,5)P 3 at the cell poles and PtdIns(4,5)P 2 at the cleavage furrow controls the polarity of the actin cytoskeleton leading to symmetrical cell division. In the endosomal compartment, the trafficking of PtdIns(3)P positive vesicles allows the recruitment of the protein machinery required for the abscission.

Evaluation of selective control information detection scheme in orthogonal frequency division multiplexing-based radio-over-fiber and visible light communication links

NASA Astrophysics Data System (ADS)

Dalarmelina, Carlos A.; Adegbite, Saheed A.; Pereira, Esequiel da V.; Nunes, Reginaldo B.; Rocha, Helder R. O.; Segatto, Marcelo E. V.; Silva, Jair A. L.

2017-05-01

Block-level detection is required to decode what may be classified as selective control information (SCI) such as control format indicator in 4G-long-term evolution systems. Using optical orthogonal frequency division multiplexing over radio-over-fiber (RoF) links, we report the experimental evaluation of an SCI detection scheme based on a time-domain correlation (TDC) technique in comparison with the conventional maximum likelihood (ML) approach. When compared with the ML method, it is shown that the TDC method improves detection performance over both 20 and 40 km of standard single mode fiber (SSMF) links. We also report a performance analysis of the TDC scheme in noisy visible light communication channel models after propagation through 40 km of SSMF. Experimental and simulation results confirm that the TDC method is attractive for practical orthogonal frequency division multiplexing-based RoF and fiber-wireless systems. Unlike the ML method, another key benefit of the TDC is that it requires no channel estimation.

The subcortical maternal complex controls symmetric division of mouse zygotes by regulating F-actin dynamics.

PubMed

Yu, Xing-Jiang; Yi, Zhaohong; Gao, Zheng; Qin, Dandan; Zhai, Yanhua; Chen, Xue; Ou-Yang, Yingchun; Wang, Zhen-Bo; Zheng, Ping; Zhu, Min-Sheng; Wang, Haibin; Sun, Qing-Yuan; Dean, Jurrien; Li, Lei

2014-09-11

Maternal effect genes play critical roles in early embryogenesis of model organisms where they have been intensively investigated. However, their molecular function in mammals remains largely unknown. Recently, we identified a subcortical maternal complex (SCMC) that contains four proteins encoded by maternal effect genes (Mater, Filia, Floped and Tle6). Here we report that TLE6, similar to FLOPED and MATER, stabilizes the SCMC and is necessary for cleavage beyond the two-cell stage of development. We document that the SCMC is required for formation of the cytoplasmic F-actin meshwork that controls the central position of the spindle and ensures symmetric division of mouse zygotes. We further demonstrate that the SCMC controls formation of the actin cytoskeleton specifically via Cofilin, a key regulator of F-actin assembly. Our results provide molecular insight into the physiological function of TLE6, its interaction with the SCMC and their roles in the symmetric division of the zygote in early mouse development.

Dissecting a new connection between cytokinin and jasmonic acid in control of leaf growth

USDA-ARS?s Scientific Manuscript database

Plant growth is mediated by two cellular processes: division and elongation. The maize leaf is an excellent model to study plant growth since these processes are spatially separated into discreet zones - a division zone (DZ), transition zone (TZ), and elongation zone (EZ) - at the base of the leaf. ...

78 FR 66279 - Significant New Use Rules on Certain Chemical Substances; Removal of Significant New Use Rules

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-05

....gov or at the Office of Pollution Prevention and Toxics Docket (OPPT Docket), Environmental Protection... information contact: Kenneth Moss, Chemical Control Division (7405M), Office of Pollution Prevention and... Division, Office of Pollution Prevention and Toxics. Therefore, 40 CFR parts 9 and 721 are amended as...

Strategic Planning in a Government Organization: The Experience of the Technical Information Division at NRaD.

ERIC Educational Resources Information Center

Cathcart, Margaret E.

1997-01-01

Describes development of strategic objectives at the Research, Development, Test, and Evaluation Division of the Naval Command, Control and Ocean Surveillance Center (NRaD). Discusses its plan to achieve these objectives. States that the organization's responsibilities span the continuum of information using technical communication skills. Uses…

ZapE Is a Novel Cell Division Protein Interacting with FtsZ and Modulating the Z-Ring Dynamics

PubMed Central

Marteyn, Benoit S.; Karimova, Gouzel; Fenton, Andrew K.; Gazi, Anastasia D.; West, Nicholas; Touqui, Lhousseine; Prevost, Marie-Christine; Betton, Jean-Michel; Poyraz, Oemer; Ladant, Daniel; Gerdes, Kenn; Sansonetti, Philippe J.; Tang, Christoph M.

2014-01-01

ABSTRACT Bacterial cell division requires the formation of a mature divisome complex positioned at the midcell. The localization of the divisome complex is determined by the correct positioning, assembly, and constriction of the FtsZ ring (Z-ring). Z-ring constriction control remains poorly understood and (to some extent) controversial, probably due to the fact that this phenomenon is transient and controlled by numerous factors. Here, we characterize ZapE, a novel ATPase found in Gram-negative bacteria, which is required for growth under conditions of low oxygen, while loss of zapE results in temperature-dependent elongation of cell shape. We found that ZapE is recruited to the Z-ring during late stages of the cell division process and correlates with constriction of the Z-ring. Overexpression or inactivation of zapE leads to elongation of Escherichia coli and affects the dynamics of the Z-ring during division. In vitro, ZapE destabilizes FtsZ polymers in an ATP-dependent manner. PMID:24595368

Industrial workers' health and environmental pollution under the new international division of labor: the Taiwan experience.

PubMed Central

Chen, M S; Huang, C L

1997-01-01

Using Taiwan as an example, this paper conducts a historical analysis of the relationship between economic development in the new international division of labor and environmental pollution and industrial workers' health. Three industries-asbestos, plastic, and dye-were chosen for case studies. We trace the emergence of each industry in Taiwan and study each industry's protection of workers' health and environmental quality. Under the new international division of labor, the state's prioritization of economic development leads to lenient regulation. Under such state policies, employers have few incentives to invest in the protection of their workers' health and in the control of environmental pollution. Workers and the public are constrained in their efforts to protect their own health and prevent environmental pollution. This situation is exemplified by the deplorable working conditions and inadequate environmental pollution controls in the asbestos, plastic, and dye industries. Workers' health and the public's health are greatly compromised by economic development in the new international division of labor. Images p1228-a p1228-b PMID:9240119

Crown-root morphology of lower incisors in patients with class III malocclusion.

PubMed

Wang, Bo; Shen, Guofang; Fang, Bing; Zhang, Li

2012-07-01

The purpose of this study was to investigate the crown-root morphology of lower incisors in patients with class III malocclusion using cone-beam computed tomography. Cone-beam computed tomography images were analyzed from 53 adult class I patients (group 1), 37 preadolescent class III patients (group 2), and 66 adult class III patients (group 3) comprising 3 divisions (divisions 1, 2, and 3 corresponded to mild, moderate, and severe class III malocclusions). The size and crown-root angulations of lower incisors in different groups and divisions were statistically appraised with group 1 used as the control group. No significant differences were found for the size of lower incisors among different groups and divisions (P > 0.05). Compared with group 1, the crown-root angulations of lower incisors in groups 2 and 3 were significantly larger (P < 0.01), and among 3 divisions, the lower incisors of division 3 rather than divisions 2 and 3 exhibited larger crown-root angulations (P < 0.01). Attention should be paid to the enlarged crown-root angulations of lower incisors in class III patients during orthodontic and orthognathic treatment, especially in severe ones.

Cell size control and homeostasis in bacteria

NASA Astrophysics Data System (ADS)

Bradde, Serena; Taheri, Sattar; Sauls, John; Hill, Nobert; Levine, Petra; Paulsson, Johan; Vergassola, Massimo; Jun, Suckjoon

2015-03-01

How cells control their size is a fundamental question in biology. The mechanisms for sensing size, time, or a combination of the two are not supported by experimental evidence. By analysing distributions of size at division at birth and generation time of hundreds of thousands of Gram-negative E. coli and Gram-positive B. subtilis cells under a wide range of tightly controlled steady-state growth conditions, we are now in the position to validate different theoretical models. In this talk I will present all possible models in details and present a general mechanism that quantitatively explains all measurable aspects of growth and cell division at both population and single-cell levels.

Myc/Mycn-mediated glycolysis enhances mouse spermatogonial stem cell self-renewal

PubMed Central

Kanatsu-Shinohara, Mito; Tanaka, Takashi; Ogonuki, Narumi; Ogura, Atsuo; Morimoto, Hiroko; Cheng, Pei Feng; Eisenman, Robert N.; Trumpp, Andreas; Shinohara, Takashi

2016-01-01

Myc plays critical roles in the self-renewal division of various stem cell types. In spermatogonial stem cells (SSCs), Myc controls SSC fate decisions because Myc overexpression induces enhanced self-renewal division, while depletion of Max, a Myc-binding partner, leads to meiotic induction. However, the mechanism by which Myc acts on SSC fate is unclear. Here we demonstrate a critical link between Myc/Mycn gene activity and glycolysis in SSC self-renewal. In SSCs, Myc/Mycn are regulated by Foxo1, whose deficiency impairs SSC self-renewal. Myc/Mycn-deficient SSCs not only undergo limited self-renewal division but also display diminished glycolytic activity. While inhibition of glycolysis decreased SSC activity, chemical stimulation of glycolysis or transfection of active Akt1 or Pdpk1 (phosphoinositide-dependent protein kinase 1 ) augmented self-renewal division, and long-term SSC cultures were derived from a nonpermissive strain that showed limited self-renewal division. These results suggested that Myc-mediated glycolysis is an important factor that increases the frequency of SSC self-renewal division. PMID:28007786

Myc/Mycn-mediated glycolysis enhances mouse spermatogonial stem cell self-renewal.

PubMed

Kanatsu-Shinohara, Mito; Tanaka, Takashi; Ogonuki, Narumi; Ogura, Atsuo; Morimoto, Hiroko; Cheng, Pei Feng; Eisenman, Robert N; Trumpp, Andreas; Shinohara, Takashi

2016-12-01

Myc plays critical roles in the self-renewal division of various stem cell types. In spermatogonial stem cells (SSCs), Myc controls SSC fate decisions because Myc overexpression induces enhanced self-renewal division, while depletion of Max, a Myc-binding partner, leads to meiotic induction. However, the mechanism by which Myc acts on SSC fate is unclear. Here we demonstrate a critical link between Myc/Mycn gene activity and glycolysis in SSC self-renewal. In SSCs, Myc/Mycn are regulated by Foxo1, whose deficiency impairs SSC self-renewal. Myc/Mycn-deficient SSCs not only undergo limited self-renewal division but also display diminished glycolytic activity. While inhibition of glycolysis decreased SSC activity, chemical stimulation of glycolysis or transfection of active Akt1 or Pdpk1 (phosphoinositide-dependent protein kinase 1 ) augmented self-renewal division, and long-term SSC cultures were derived from a nonpermissive strain that showed limited self-renewal division. These results suggested that Myc-mediated glycolysis is an important factor that increases the frequency of SSC self-renewal division. © 2016 Kanatsu-Shinohara et al.; Published by Cold Spring Harbor Laboratory Press.

Mammalian aPKC/Par polarity complex mediated regulation of epithelial division orientation and cell fate

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vorhagen, Susanne; Niessen, Carien M., E-mail: carien.niessen@uni-koeln.de

2014-11-01

Oriented cell division is a key regulator of tissue architecture and crucial for morphogenesis and homeostasis. Balanced regulation of proliferation and differentiation is an essential property of tissues not only to drive morphogenesis but also to maintain and restore homeostasis. In many tissues orientation of cell division is coupled to the regulation of differentiation producing daughters with similar (symmetric cell division, SCD) or differential fate (asymmetric cell division, ACD). This allows the organism to generate cell lineage diversity from a small pool of stem and progenitor cells. Division orientation and/or the ratio of ACD/SCD need to be tightly controlled. Lossmore » of orientation or an altered ratio can promote overgrowth, alter tissue architecture and induce aberrant differentiation, and have been linked to morphogenetic diseases, cancer and aging. A key requirement for oriented division is the presence of a polarity axis, which can be established through cell intrinsic and/or extrinsic signals. Polarity proteins translate such internal and external cues to drive polarization. In this review we will focus on the role of the polarity complex aPKC/Par3/Par6 in the regulation of division orientation and cell fate in different mammalian epithelia. We will compare the conserved function of this complex in mitotic spindle orientation and distribution of cell fate determinants and highlight common and differential mechanisms in which this complex is used by tissues to adapt division orientation and cell fate to the specific properties of the epithelium.« less

Business Opportunities Guide

DTIC Science & Technology

1987-01-01

Electronic Systems Division Business Opportunities Guide. We remain committed to providing industry the best information possible on pending ESD...Commander Justific ill,):, By_ D.is’ * . ’ . Dist, : :,. ELECTRONIC SYSTEMS DIVISION BUSINESS OPPORTUNITIES GUIDE The complex Command, Control...Communications (C) systems necessary for tomorrow’s Air Foice can only be developed by a stronq’ESD/industry team. Our first Business Opportunities Guide

75 FR 60490 - 60-Day Notice of Proposed Information Collection: Bureau of Educational and Cultural Affairs...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-30

... Policy and Evaluation, Evaluation Division Survey Question Bank. OMB Control Number: 1405-0158. Type of... Cultural Affairs, Office of Policy and Evaluation, Evaluation Division (ECA/P/ V). Form Number: New surveys...-ROM submissions): ECA/P/V, SA-5, 5th Floor, Department of State, Washington, DC 20522-0505. Fax: 202...

Sporulation-specific cell division defects in ylmE mutants of Streptomyces coelicolor are rescued by additional deletion of ylmD.

PubMed

Zhang, Le; Willemse, Joost; Hoskisson, Paul A; van Wezel, Gilles P

2018-05-09

Cell division during the reproductive phase of the Streptomyces life-cycle requires tight coordination between synchronous formation of multiple septa and DNA segregation. One remarkable difference with most other bacterial systems is that cell division in Streptomyces is positively controlled by the recruitment of FtsZ by SsgB. Here we show that deletion of ylmD (SCO2081) or ylmE (SCO2080), which lie in operon with ftsZ in the dcw cluster of actinomycetes, has major consequences for sporulation-specific cell division in Streptomyces coelicolor. Electron and fluorescence microscopy demonstrated that ylmE mutants have a highly aberrant phenotype with defective septum synthesis, and produce very few spores with low viability and high heat sensitivity. FtsZ-ring formation was also highly disturbed in ylmE mutants. Deletion of ylmD had a far less severe effect on sporulation. Interestingly, the additional deletion of ylmD restored sporulation to the ylmE null mutant. YlmD and YlmE are not part of the divisome, but instead localize diffusely in aerial hyphae, with differential intensity throughout the sporogenic part of the hyphae. Taken together, our work reveals a function for YlmD and YlmE in the control of sporulation-specific cell division in S. coelicolor, whereby the presence of YlmD alone results in major developmental defects.

Stable Regulation of Cell Cycle Events in Mycobacteria: Insights From Inherently Heterogeneous Bacterial Populations.

PubMed

Logsdon, Michelle M; Aldridge, Bree B

2018-01-01

Model bacteria, such as E. coli and B. subtilis , tightly regulate cell cycle progression to achieve consistent cell size distributions and replication dynamics. Many of the hallmark features of these model bacteria, including lateral cell wall elongation and symmetric growth and division, do not occur in mycobacteria. Instead, mycobacterial growth is characterized by asymmetric polar growth and division. This innate asymmetry creates unequal birth sizes and growth rates for daughter cells with each division, generating a phenotypically heterogeneous population. Although the asymmetric growth patterns of mycobacteria lead to a larger variation in birth size than typically seen in model bacterial populations, the cell size distribution is stable over time. Here, we review the cellular mechanisms of growth, division, and cell cycle progression in mycobacteria in the face of asymmetry and inherent heterogeneity. These processes coalesce to control cell size. Although Mycobacterium smegmatis and Mycobacterium bovis Bacillus Calmette-Guérin (BCG) utilize a novel model of cell size control, they are similar to previously studied bacteria in that initiation of DNA replication is a key checkpoint for cell division. We compare the regulation of DNA replication initiation and strategies used for cell size homeostasis in mycobacteria and model bacteria. Finally, we review the importance of cellular organization and chromosome segregation relating to the physiology of mycobacteria and consider how new frameworks could be applied across the wide spectrum of bacterial diversity.

Gain control through divisive inhibition prevents abrupt transition to chaos in a neural mass model.

PubMed

Papasavvas, Christoforos A; Wang, Yujiang; Trevelyan, Andrew J; Kaiser, Marcus

2015-09-01

Experimental results suggest that there are two distinct mechanisms of inhibition in cortical neuronal networks: subtractive and divisive inhibition. They modulate the input-output function of their target neurons either by increasing the input that is needed to reach maximum output or by reducing the gain and the value of maximum output itself, respectively. However, the role of these mechanisms on the dynamics of the network is poorly understood. We introduce a novel population model and numerically investigate the influence of divisive inhibition on network dynamics. Specifically, we focus on the transitions from a state of regular oscillations to a state of chaotic dynamics via period-doubling bifurcations. The model with divisive inhibition exhibits a universal transition rate to chaos (Feigenbaum behavior). In contrast, in an equivalent model without divisive inhibition, transition rates to chaos are not bounded by the universal constant (non-Feigenbaum behavior). This non-Feigenbaum behavior, when only subtractive inhibition is present, is linked to the interaction of bifurcation curves in the parameter space. Indeed, searching the parameter space showed that such interactions are impossible when divisive inhibition is included. Therefore, divisive inhibition prevents non-Feigenbaum behavior and, consequently, any abrupt transition to chaos. The results suggest that the divisive inhibition in neuronal networks could play a crucial role in keeping the states of order and chaos well separated and in preventing the onset of pathological neural dynamics.

Individuality and universality in the growth-division laws of single E. coli cells

NASA Astrophysics Data System (ADS)

Kennard, Andrew S.; Osella, Matteo; Javer, Avelino; Grilli, Jacopo; Nghe, Philippe; Tans, Sander J.; Cicuta, Pietro; Cosentino Lagomarsino, Marco

2016-01-01

The mean size of exponentially dividing Escherichia coli cells in different nutrient conditions is known to depend on the mean growth rate only. However, the joint fluctuations relating cell size, doubling time, and individual growth rate are only starting to be characterized. Recent studies in bacteria reported a universal trend where the spread in both size and doubling times is a linear function of the population means of these variables. Here we combine experiments and theory and use scaling concepts to elucidate the constraints posed by the second observation on the division control mechanism and on the joint fluctuations of sizes and doubling times. We found that scaling relations based on the means collapse both size and doubling-time distributions across different conditions and explain how the shape of their joint fluctuations deviates from the means. Our data on these joint fluctuations highlight the importance of cell individuality: Single cells do not follow the dependence observed for the means between size and either growth rate or inverse doubling time. Our calculations show that these results emerge from a broad class of division control mechanisms requiring a certain scaling form of the "division hazard rate function," which defines the probability rate of dividing as a function of measurable parameters. This "model free" approach gives a rationale for the universal body-size distributions observed in microbial ecosystems across many microbial species, presumably dividing with multiple mechanisms. Additionally, our experiments show a crossover between fast and slow growth in the relation between individual-cell growth rate and division time, which can be understood in terms of different regimes of genome replication control.

Cell division and endoreduplication: doubtful engines of vegetative growth.

PubMed

John, Peter C L; Qi, Ruhu

2008-03-01

Currently, there is little information to indicate whether plant cell division and development is the collective effect of individual cell programming (cell-based) or is determined by organ-wide growth (organismal). Modulation of cell division does not confirm cell autonomous programming of cell expansion; instead, final cell size seems to be determined by the balance between cells formed and subsequent tissue growth. Control of growth in regions of the plant therefore has great importance in determining cell, organ and plant development. Here, we question the view that formation of new cells and their programmed expansion is the driving force of growth. We believe there is evidence that division does not drive, but requires, cell growth and a similar requirement for growth is detected in the modified cycle termed endoreduplication.

CoMIC, the hidden dynamics of mitochondrial inner compartments

PubMed Central

Cho, Bongki; Sun, Woong

2017-01-01

Mitochondria have evolutionarily, functionally and structurally distinct outer- (OMM) and inner-membranes (IMM). Thus, mitochondrial morphology is controlled by independent but coordinated activity of fission and fusion of the OMM and IMM. Constriction and division of the OMM are mediated by endocytosis-like machineries, which include dynamin-related protein 1 with additional cytosolic vesicle scissoring machineries such as actin filament and Dynamin 2. However, structural alteration of the IMM during mitochondrial division has been poorly understood. Recently, we found that the IMM and the inner compartments undergo transient and reversible constriction prior to the OMM division, which we termed CoMIC, Constriction of Mitochondrial Inner Compartment. In this short review, we further discuss the evolutionary perspective and the regulatory mechanism of CoMIC during mitochondrial division. PMID:28803609

CoMIC, the hidden dynamics of mitochondrial inner compartments.

PubMed

Cho, Bongki; Sun, Woong

2017-12-01

Mitochondria have evolutionarily, functionally and structurally distinct outer- (OMM) and inner-membranes (IMM). Thus, mitochondrial morphology is controlled by independent but coordinated activity of fission and fusion of the OMM and IMM. Constriction and division of the OMM are mediated by endocytosis-like machineries, which include dynamin-related protein 1 with additional cytosolic vesicle scissoring machineries such as actin filament and Dynamin 2. However, structural alteration of the IMM during mitochondrial division has been poorly understood. Recently, we found that the IMM and the inner compartments undergo transient and reversible constriction prior to the OMM division, which we termed CoMIC, Constriction of Mitochondrial Inner Compartment. In this short review, we further discuss the evolutionary perspective and the regulatory mechanism of CoMIC during mitochondrial division. [BMB Reports 2017; 50(12): 597-598].

Measuring Clinical Productivity.

PubMed

Hudson, Mark E; Lebovitz, Evan E

2018-06-01

Productivity measurements have been used to evaluate and compare physicians and physician practices. Anesthesiology is unique in that factors outside anesthesiologist control impact opportunity for revenue generation and make comparisons between providers and facilities challenging. This article uses data from the multicenter University of Pittsburgh Physicians Department of Anesthesiology to demonstrate factors influencing productivity opportunity by surgical facility, between department divisions and subspecialties within multispecialty divisions, and by individuals within divisions. The complexities of benchmarking anesthesiology productivity are demonstrated, and the potential value of creating a productivity profile for facilities and groups is illustrated. Copyright © 2018 Elsevier Inc. All rights reserved.

An Autonomous Satellite Time Synchronization System Using Remotely Disciplined VC-OCXOs

PubMed Central

Gu, Xiaobo; Chang, Qing; Glennon, Eamonn P.; Xu, Baoda; Dempseter, Andrew G.; Wang, Dun; Wu, Jiapeng

2015-01-01

An autonomous remote clock control system is proposed to provide time synchronization and frequency syntonization for satellite to satellite or ground to satellite time transfer, with the system comprising on-board voltage controlled oven controlled crystal oscillators (VC-OCXOs) that are disciplined to a remote master atomic clock or oscillator. The synchronization loop aims to provide autonomous operation over extended periods, be widely applicable to a variety of scenarios and robust. A new architecture comprising the use of frequency division duplex (FDD), synchronous time division (STDD) duplex and code division multiple access (CDMA) with a centralized topology is employed. This new design utilizes dual one-way ranging methods to precisely measure the clock error, adopts least square (LS) methods to predict the clock error and employs a third-order phase lock loop (PLL) to generate the voltage control signal. A general functional model for this system is proposed and the error sources and delays that affect the time synchronization are discussed. Related algorithms for estimating and correcting these errors are also proposed. The performance of the proposed system is simulated and guidance for selecting the clock is provided. PMID:26213929

Hematopoietic stem cells can differentiate into restricted myeloid progenitors before cell division in mice.

PubMed

Grinenko, Tatyana; Eugster, Anne; Thielecke, Lars; Ramasz, Beáta; Krüger, Anja; Dietz, Sevina; Glauche, Ingmar; Gerbaulet, Alexander; von Bonin, Malte; Basak, Onur; Clevers, Hans; Chavakis, Triantafyllos; Wielockx, Ben

2018-05-15

Hematopoietic stem cells (HSCs) continuously replenish all blood cell types through a series of differentiation steps and repeated cell divisions that involve the generation of lineage-committed progenitors. However, whether cell division in HSCs precedes differentiation is unclear. To this end, we used an HSC cell-tracing approach and Ki67 RFP knock-in mice, in a non-conditioned transplantation model, to assess divisional history, cell cycle progression, and differentiation of adult HSCs. Our results reveal that HSCs are able to differentiate into restricted progenitors, especially common myeloid, megakaryocyte-erythroid and pre-megakaryocyte progenitors, without undergoing cell division and even before entering the S phase of the cell cycle. Additionally, the phenotype of the undivided but differentiated progenitors correlated with the expression of lineage-specific genes and loss of multipotency. Thus HSC fate decisions can be uncoupled from physical cell division. These results facilitate a better understanding of the mechanisms that control fate decisions in hematopoietic cells.

Direct interaction of FtsZ and MreB is required for septum synthesis and cell division in Escherichia coli.

PubMed

Fenton, Andrew K; Gerdes, Kenn

2013-07-03

How bacteria coordinate cell growth with division is not well understood. Bacterial cell elongation is controlled by actin-MreB while cell division is governed by tubulin-FtsZ. A ring-like structure containing FtsZ (the Z ring) at mid-cell attracts other cell division proteins to form the divisome, an essential protein assembly required for septum synthesis and cell separation. The Z ring exists at mid-cell during a major part of the cell cycle without contracting. Here, we show that MreB and FtsZ of Escherichia coli interact directly and that this interaction is required for Z ring contraction. We further show that the MreB-FtsZ interaction is required for transfer of cell-wall biosynthetic enzymes from the lateral to the mature divisome, allowing cells to synthesise the septum. Our observations show that bacterial cell division is coupled to cell elongation via a direct and essential interaction between FtsZ and MreB.

Activity and Accumulation of Cell Division-Promoting Phenolics in Tobacco Tissue Cultures 1

PubMed Central

Teutonico, Rita A.; Dudley, Matthew W.; Orr, John D.; Lynn, David G.; Binns, Andrew N.

1991-01-01

Dehydrodiconiferyl alcohol glucosides (DCGs) are derivatives of the phenylpropanoid pathway that have been isolated from Catharansus roseus L. (Vinca rosea) crown gall tumors. Fractions containing purified DCGs have been shown previously to promote the growth of cytokinin-requiring tissues of tobacco in the absence of exogenous cytokinins. In this study, we utilized synthetic DCG isomers to confirm the cell division-promoting activity of DCG isomers A and B and show that they neither promote shoot meristem initiation on Nicotiana tabacum L., cv Havana 425, leaf explants nor induce betacyanin synthesis in amaranth seedlings. Analysis of cultured tobacco pith tissue demonstrated that DCG accumulation was stimulated by cytokinin treatment and correlated with cytokinin-induced cell division. Thus, the accumulation of metabolites that could replace cytokinin in cell division bioassays is stimulated by cytokinins. These data support the model that DCGs are a component of a cytokinin-mediated regulatory circuit controlling cell division. ImagesFigure 2 PMID:16668384

Direct interaction of FtsZ and MreB is required for septum synthesis and cell division in Escherichia coli

PubMed Central

Fenton, Andrew K; Gerdes, Kenn

2013-01-01

How bacteria coordinate cell growth with division is not well understood. Bacterial cell elongation is controlled by actin–MreB while cell division is governed by tubulin–FtsZ. A ring-like structure containing FtsZ (the Z ring) at mid-cell attracts other cell division proteins to form the divisome, an essential protein assembly required for septum synthesis and cell separation. The Z ring exists at mid-cell during a major part of the cell cycle without contracting. Here, we show that MreB and FtsZ of Escherichia coli interact directly and that this interaction is required for Z ring contraction. We further show that the MreB–FtsZ interaction is required for transfer of cell-wall biosynthetic enzymes from the lateral to the mature divisome, allowing cells to synthesise the septum. Our observations show that bacterial cell division is coupled to cell elongation via a direct and essential interaction between FtsZ and MreB. PMID:23756461

OSD1 promotes meiotic progression via APC/C inhibition and forms a regulatory network with TDM and CYCA1;2/TAM.

PubMed

Cromer, Laurence; Heyman, Jefri; Touati, Sandra; Harashima, Hirofumi; Araou, Emilie; Girard, Chloe; Horlow, Christine; Wassmann, Katja; Schnittger, Arp; De Veylder, Lieven; Mercier, Raphael

2012-01-01

Cell cycle control is modified at meiosis compared to mitosis, because two divisions follow a single DNA replication event. Cyclin-dependent kinases (CDKs) promote progression through both meiosis and mitosis, and a central regulator of their activity is the APC/C (Anaphase Promoting Complex/Cyclosome) that is especially required for exit from mitosis. We have shown previously that OSD1 is involved in entry into both meiosis I and meiosis II in Arabidopsis thaliana; however, the molecular mechanism by which OSD1 controls these transitions has remained unclear. Here we show that OSD1 promotes meiotic progression through APC/C inhibition. Next, we explored the functional relationships between OSD1 and the genes known to control meiotic cell cycle transitions in Arabidopsis. Like osd1, cyca1;2/tam mutation leads to a premature exit from meiosis after the first division, while tdm mutants perform an aberrant third meiotic division after normal meiosis I and II. Remarkably, while tdm is epistatic to tam, osd1 is epistatic to tdm. We further show that the expression of a non-destructible CYCA1;2/TAM provokes, like tdm, the entry into a third meiotic division. Finally, we show that CYCA1;2/TAM forms an active complex with CDKA;1 that can phosphorylate OSD1 in vitro. We thus propose that a functional network composed of OSD1, CYCA1;2/TAM, and TDM controls three key steps of meiotic progression, in which OSD1 is a meiotic APC/C inhibitor.

Incidence of chronic groin pain following open mesh inguinal hernia repair, and effect of elective division of the ilioinguinal nerve: meta-analysis of randomized controlled trials.

PubMed

Charalambous, M P; Charalambous, C P

2018-06-01

Chronic post-operative groin pain is a substantial complication following open mesh inguinal hernia repair. The exact cause of this pain is still unclear, but entrapment or trauma of the ilioinguinal nerve may have a role to play. Elective division of this nerve during hernia repair has been proposed in an attempt to reduce the incidence of chronic groin pain. We performed a meta-analysis of nine randomized controlled trials comparing preservation versus elective division of the ilioinguinal nerve during this operation. A substantial proportion of patients having open mesh inguinal hernia repair experience chronic groin pain when the ilioinguinal nerve is preserved (estimated rate of 9.4% at 6 months and 4.8% at 1 year). Elective division of the nerve resulted in a significant reduction of groin pain at 6-months post-surgery (RR 0.47, p = 0.02), including moderate/severe pain (RR 0.57, p = 0.01). However, division of the nerve also resulted in an increase of subjective groin numbness at this time point (RR 1.55, p = 0.06). At 12-month post-surgery, the beneficial effect of nerve division on chronic pain was reduced, with no significant difference in the rates of overall groin pain (RR 0.69, p = 0.38), or of moderate-to-severe groin pain (RR 0.99, p = 0.98) between the two groups. The prevalence of groin numbness was also similar between the two groups at 12-month post-surgery (RR 0.79, p = 0.48). Routine elective division of the ilioinguinal nerve during open mesh inguinal hernia repair does not significantly reduce chronic groin pain beyond 6 months, and may result in increased rates of groin numbness, especially in the first 6-months post-surgery.

76 FR 63899 - Positive Train Control Systems

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-14

...-0028, Notice No. 2] RIN 2130-AC27 Positive Train Control Systems AGENCY: Federal Railroad...-based criteria in order to avoid positive train control (PTC) system implementation on track segments... and Compliance, Staff Director, Signal & Train Control Division, Federal Railroad Administration, Mail...

Mountain-Plains Handbook: The Design and Operation of a Residential Family Based Education Program. Appendix. Supplement II to Volume 5. Operational Support: Administrative Services Division.

ERIC Educational Resources Information Center

Anderson, Newell B.; And Others

One of two supplements which accompany chapter 5 of "Mountain-Plains Handbook: The Design and Operation of a Residential, Family Oriented Career Education Model" (CE 014 630), this document contains specific information concerning the following components of the administrative services division: purchasing, property control, and…

76 FR 26721 - Re-Issuance of a General Permit to the National Science Foundation for the Ocean Disposal of Man...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-09

... Scientist, Marine Pollution Control Branch, Oceans and Coastal Protection Division (4504T), U.S... requires the NSF to report by June 30 of every year to the Director of the Oceans and Coastal Protection... the Director of the Oceans and Coastal Protection Division, in the Office of Water, at EPA...

78 FR 39772 - Huntingdon County Site, FCI USA, LLC, Americas Division, a Subsidiary of FCI SA, Including On...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-02

... USA, LLC. The Department has determined that these workers were sufficiently under the control of the..., FCI USA, LLC, Americas Division, a Subsidiary of FCI SA, Including On-Site Leased Workers From... Eligibility To Apply for Worker Adjustment Assistance In accordance with Section 223 of the Trade Act of 1974...

National Program for Inspection of Non-Federal Dams. Millen Lake Dam (NH 00236), NHWRB Number 245.04, Connecticut River Basin, Washington, New Hampshire. Phase I Inspection Report.

DTIC Science & Technology

1980-03-01

Engineering Branch Engineering Division CARNEY M. TERZIAN, KENBER Design Branch Engineering Division RICHARD DIBKO CHIRA Water Control Branch...State of New Hampshire. Authorization and notice to proceed were issued to S E A Consultants Inc. under a letter of November 5, 1979 from William

UGV Interoperability Profile (IOP) Communications Profile, Version 0

DTIC Science & Technology

2011-12-21

some UGV systems employ Orthogonal Frequency Division Multiplexing ( OFDM ) or Coded Orthogonal Frequency Division Multiplexing (COFDM) waveforms which...other portions of the IOP. Attribute Paragraph Title Values Waveform 3.3 Air Interface/ Waveform OFDM , COFDM, DDL, CDL, None OCU to Platform...Sight MANET Mobile Ad-hoc Network Mbps Megabits per second MC/PM Master Controller/ Payload Manager MHz Megahertz MIMO Multiple Input Multiple

GLABROUS INFLORESCENCE STEMS regulates trichome branching by genetically interacting with SIM in Arabidopsis.

PubMed

Sun, Li-Li; Zhou, Zhong-Jing; An, Li-Jun; An, Yan; Zhao, Yong-Qin; Meng, Xiao-Fang; Steele-King, Clare; Gan, Yin-Bo

2013-07-01

Arabidopsis trichomes are large branched single cells that protrude from the epidermis. The first morphological indication of trichome development is an increase in nuclear content resulting from an initial cycle of endoreduplication. Our previous study has shown that the C2H2 zinc finger protein GLABROUS INFLORESCENCE STEMS (GIS) is required for trichome initiation in the inflorescence organ and for trichome branching in response to gibberellic acid signaling, although GIS gene does not play a direct role in regulating trichome cell division. Here, we describe a novel role of GIS, controlling trichome cell division indirectly by interacting genetically with a key endoreduplication regulator SIAMESE (SIM). Our molecular and genetic studies have shown that GIS might indireclty control cell division and trichome branching by acting downstream of SIM. A loss of function mutation of SIM signficantly reduced the expression of GIS. Futhermore, the overexpression of GIS rescued the trichome cluster cell phenotypes of sim mutant. The gain or loss of function of GIS had no significant effect on the expression of SIM. These results suggest that GIS may play an indirect role in regulating trichome cell division by genetically interacting with SIM.

On the interrelation of multiplication and division in secondary school children

PubMed Central

Huber, Stefan; Fischer, Ursula; Moeller, Korbinian; Nuerk, Hans-Christoph

2013-01-01

Multiplication and division are conceptually inversely related: Each division problem can be transformed into as a multiplication problem and vice versa. Recent research has indicated strong developmental parallels between multiplication and division in primary school children. In this study, we were interested in (i) whether these developmental parallels persist into secondary school, (ii) whether similar developmental parallels can be observed for simple and complex problems, (iii) whether skill level modulates this relationship, and (iv) whether the correlations are specific and not driven by general cognitive or arithmetic abilities. Therefore, we assessed performance of 5th and 6th graders attending two secondary school types of the German educational system in simple and complex multiplication as well as division while controlling for non-verbal intelligence, short-term memory, and other arithmetic abilities. Accordingly, we collected data from students differing in skills levels due to either age (5th < 6th grade) or school type (general < intermediate secondary school). We observed moderate to strong bivariate and partial correlations between multiplication and division with correlations being higher for simple tasks but nevertheless reliable for complex tasks. Moreover, the association between simple multiplication and division depended on students' skill levels as reflected by school types, but not by age. Partial correlations were higher for intermediate than for general secondary school children. In sum, these findings emphasize the importance of the inverse relationship between multiplication and division which persists into later developmental stages. However, evidence for skill-related differences in the relationship between multiplication and division was restricted to the differences for school types. PMID:24133476

Spaceport Command and Control System Software Development

NASA Technical Reports Server (NTRS)

Glasser, Abraham

2017-01-01

The Spaceport Command and Control System (SCCS) is the National Aeronautics and Space Administration's (NASA) launch control system for the Orion capsule and Space Launch System, the next generation manned rocket currently in development. This large system requires a large amount of intensive testing that will properly measure the capabilities of the system. Automating the test procedures would save the project money from human labor costs, as well as making the testing process more efficient. Therefore, the Exploration Systems Division (formerly the Electrical Engineering Division) at Kennedy Space Center (KSC) has recruited interns for the past two years to work alongside full-time engineers to develop these automated tests, as well as innovate upon the current automation process.

Hematopoietic stem cell fate through metabolic control.

PubMed

Ito, Kyoko; Ito, Keisuke

2018-05-25

Hematopoietic stem cells (HSCs) maintain a quiescent state in the bone marrow to preserve their self-renewal capacity, but also undergo cell divisions as required. Organelles such as the mitochondria sustain cumulative damage during these cell divisions, and this damage may eventually compromise the cells' self-renewal capacity. HSC divisions result in either self-renewal or differentiation, with the balance between the two directly impacting hematopoietic homeostasis; but the heterogeneity of available HSC-enriched fractions, together with the technical challenges of observing HSC behavior, has long hindered the analysis of individual HSCs, and prevented the elucidation of this process. However, recent advances in genetic models, metabolomics analyses and single-cell approaches have revealed the contributions made to HSC self-renewal by metabolic cues, mitochondrial biogenesis, and autophagy/mitophagy, which have highlighted mitochondrial quality as a key control factor in the equilibrium of HSCs. A deeper understanding of precisely how specific modes of metabolism control HSC fate at the single cell level is therefore not only of great biological interest, but will have clear clinical implications for the development of therapies for hematological disease. Copyright © 2018. Published by Elsevier Inc.

Activity in the Mission Control Center during Apollo 14

NASA Image and Video Library

1971-02-04

S71-17610 (4 Feb. 1971) --- Partial view of activity in the Mission Operations Control Room in the Mission Control Center at the time the Apollo 14 S-IVB stage impacted on the lunar surface. The flight director's console is in the foreground. Eugene F. Kranz, chief of the MSC Flight Control Division, is in the right foreground. Seated at the console is Glynn S. Lunney, head of the Flight Director Office, Flight Control Division. Facing the camera is Gerald D. Griffin, flight director of the Third (Gold) Team. A seismic reading from the impact can be seen in the center background. The S-IVB impacted on the lunar surface at 1:40:54 a.m. (CST), Feb. 4, 1971, about 90 nautical miles south-southwest of the Apollo 12 passive seismometer. The energy release was comparable to 11 tons of TNT.

The Arf GAP CNT-2 regulates the apoptotic fate in C. elegans asymmetric neuroblast divisions.

PubMed

Singhvi, Aakanksha; Teuliere, Jerome; Talavera, Karla; Cordes, Shaun; Ou, Guangshuo; Vale, Ronald D; Prasad, Brinda C; Clark, Scott G; Garriga, Gian

2011-06-07

During development, all cells make the decision to live or die. Although the molecular mechanisms that execute the apoptotic program are well defined, less is known about how cells decide whether to live or die. In C. elegans, this decision is linked to how cells divide asymmetrically [1, 2]. Several classes of molecules are known to regulate asymmetric cell divisions in metazoans, yet these molecules do not appear to control C. elegans divisions that produce apoptotic cells [3]. We identified CNT-2, an Arf GTPase-activating protein (GAP) of the AGAP family, as a novel regulator of this type of neuroblast division. Loss of CNT-2 alters daughter cell size and causes the apoptotic cell to adopt the fate of its sister cell, resulting in extra neurons. CNT-2's Arf GAP activity is essential for its function in these divisions. The N terminus of CNT-2, which contains a GTPase-like domain that defines the AGAP class of Arf GAPs, negatively regulates CNT-2's function. We provide evidence that CNT-2 regulates receptor-mediated endocytosis and consider the implications of its role in asymmetric cell divisions. Copyright © 2011 Elsevier Ltd. All rights reserved.

Similar effects of substance P on learning and memory function between hippocampus and striatal marginal division

PubMed Central

Yu, Yan; Zeng, Changchun; Shu, Siyun; Liu, Xuemei; Li, Chuhua

2014-01-01

Substance P is an endogenous neurokinin that is present in the central and peripheral nervous systems. The neuropeptide substance P and its high-affinity receptor neurokinin 1 receptor are known to play an important role in the central nervous system in inflammation, blood pressure, motor behavior and anxiety. The effects of substance P in the hippocampus and the marginal division of the striatum on memory remain poorly understood. Compared with the hippocampus as a control, immunofluorescence showed high expression of the substance P receptor, neurokinin 1, in the marginal division of the striatum of normal rats. Unilateral or bilateral injection of an antisense oligonucleotide against neurokinin 1 receptor mRNA in the rat hippocampus or marginal division of the striatum effectively reduced neurokinin 1 receptor expression. Independent of injection site, rats that received this antisense oligonucleotide showed obviously increased footshock times in a Y-maze test. These results indicate that the marginal division of the striatum plays a similar function in learning and memory to the hippocampus, which is a valuable addition to our mechanistic understanding of the learning and memory functions of the marginal division of the striatum. PMID:25206901

Variable threshold algorithm for division of labor analyzed as a dynamical system.

PubMed

Castillo-Cagigal, Manuel; Matallanas, Eduardo; Navarro, Iñaki; Caamaño-Martín, Estefanía; Monasterio-Huelin, Félix; Gutiérrez, Álvaro

2014-12-01

Division of labor is a widely studied aspect of colony behavior of social insects. Division of labor models indicate how individuals distribute themselves in order to perform different tasks simultaneously. However, models that study division of labor from a dynamical system point of view cannot be found in the literature. In this paper, we define a division of labor model as a discrete-time dynamical system, in order to study the equilibrium points and their properties related to convergence and stability. By making use of this analytical model, an adaptive algorithm based on division of labor can be designed to satisfy dynamic criteria. In this way, we have designed and tested an algorithm that varies the response thresholds in order to modify the dynamic behavior of the system. This behavior modification allows the system to adapt to specific environmental and collective situations, making the algorithm a good candidate for distributed control applications. The variable threshold algorithm is based on specialization mechanisms. It is able to achieve an asymptotically stable behavior of the system in different environments and independently of the number of individuals. The algorithm has been successfully tested under several initial conditions and number of individuals.

Gain control through divisive inhibition prevents abrupt transition to chaos in a neural mass model

PubMed Central

Papasavvas, Christoforos A.; Wang, Yujiang; Trevelyan, Andrew J.; Kaiser, Marcus

2016-01-01

Experimental results suggest that there are two distinct mechanisms of inhibition in cortical neuronal networks: subtractive and divisive inhibition. They modulate the input-output function of their target neurons either by increasing the input that is needed to reach maximum output or by reducing the gain and the value of maximum output itself, respectively. However, the role of these mechanisms on the dynamics of the network is poorly understood. We introduce a novel population model and numerically investigate the influence of divisive inhibition on network dynamics. Specifically, we focus on the transitions from a state of regular oscillations to a state of chaotic dynamics via period-doubling bifurcations. The model with divisive inhibition exhibits a universal transition rate to chaos (Feigenbaum behavior). In contrast, in an equivalent model without divisive inhibition, transition rates to chaos are not bounded by the universal constant (non-Feigenbaum behavior). This non-Feigenbaum behavior, when only subtractive inhibition is present, is linked to the interaction of bifurcation curves in the parameter space. Indeed, searching the parameter space showed that such interactions are impossible when divisive inhibition is included. Therefore, divisive inhibition prevents non-Feigenbaum behavior and, consequently, any abrupt transition to chaos. The results suggest that the divisive inhibition in neuronal networks could play a crucial role in keeping the states of order and chaos well separated and in preventing the onset of pathological neural dynamics. PMID:26465514

On the interrelation of multiplication and division in secondary school children.

PubMed

Huber, Stefan; Fischer, Ursula; Moeller, Korbinian; Nuerk, Hans-Christoph

2013-01-01

Each division problem can be transformed into as a multiplication problem and vice versa. Recent research has indicated strong developmental parallels between multiplication and division in primary school children. In this study, we were interested in (i) whether these developmental parallels persist into secondary school, (ii) whether similar developmental parallels can be observed for simple and complex problems, (iii) whether skill level modulates this relationship, and (iv) whether the correlations are specific and not driven by general cognitive or arithmetic abilities. Therefore, we assessed performance of 5th and 6th graders attending two secondary school types of the German educational system in simple and complex multiplication as well as division while controlling for non-verbal intelligence, short-term memory, and other arithmetic abilities. Accordingly, we collected data from students differing in skills levels due to either age (5th < 6th grade) or school type (general < intermediate secondary school). We observed moderate to strong bivariate and partial correlations between multiplication and division with correlations being higher for simple tasks but nevertheless reliable for complex tasks. Moreover, the association between simple multiplication and division depended on students' skill levels as reflected by school types, but not by age. Partial correlations were higher for intermediate than for general secondary school children. In sum, these findings emphasize the importance of the inverse relationship between multiplication and division which persists into later developmental stages. However, evidence for skill-related differences in the relationship between multiplication and division was restricted to the differences for school types.

77 FR 28285 - Positive Train Control Systems (RRR)

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-14

...-0028, Notice No. 3] RIN 2130-AC27 Positive Train Control Systems (RRR) AGENCY: Federal Railroad... railroads to install positive train control (PTC) systems. This final rule removes regulatory provisions... Safety Assurance and Compliance, Staff Director, Signal & Train Control Division, Federal Railroad...

Orbiter data reduction complex data processing requirements for the OFT mission evaluation team (level C)

NASA Technical Reports Server (NTRS)

1979-01-01

This document addresses requirements for post-test data reduction in support of the Orbital Flight Tests (OFT) mission evaluation team, specifically those which are planned to be implemented in the ODRC (Orbiter Data Reduction Complex). Only those requirements which have been previously baselined by the Data Systems and Analysis Directorate configuration control board are included. This document serves as the control document between Institutional Data Systems Division and the Integration Division for OFT mission evaluation data processing requirements, and shall be the basis for detailed design of ODRC data processing systems.

Proceedings of the Annual Meeting of the Association for Education in Journalism and Mass Communication (85th, Miami, Florida, August 5-8, 2002). Law Division.

ERIC Educational Resources Information Center

2002

The Law Division of the proceedings contains the following 8 papers: "Trademarks and the First Amendment: The Anatomy of a Conflict" (Retha J. Martin); "Exit Polls and Other Bad Habits: An Analysis of First Amendment Considerations Concerning Policy Recommendations to Control or Prohibit Media Election Forecasts" (Niels…

National Program for Inspection of Non-Federal Dams. Scovill Reservoir Dam (CT 00431), Lower Connecticut River Basin, Haddam, Connecticut. Phase I Inspection Report.

DTIC Science & Technology

1980-01-01

Engineering Branch Engineering Division CARNEY M. TERZIAN, MEMBER Design Branch Engineering Division S, RICHARD DIE O CHIRA Water Control Branch...Associates, P.C. under a letter of 19 October 1979 from William E. Hodgson, Jr., Colonel, Corps of Engineers. Contract No. DACW33-80-C-0001 has been assigned

LocZ Is a New Cell Division Protein Involved in Proper Septum Placement in Streptococcus pneumoniae

PubMed Central

Holečková, Nela; Molle, Virginie; Buriánková, Karolína; Benada, Oldřich; Kofroňová, Olga; Ulrych, Aleš; Branny, Pavel

2014-01-01

ABSTRACT How bacteria control proper septum placement at midcell, to guarantee the generation of identical daughter cells, is still largely unknown. Although different systems involved in the selection of the division site have been described in selected species, these do not appear to be widely conserved. Here, we report that LocZ (Spr0334), a newly identified cell division protein, is involved in proper septum placement in Streptococcus pneumoniae. We show that locZ is not essential but that its deletion results in cell division defects and shape deformation, causing cells to divide asymmetrically and generate unequally sized, occasionally anucleated, daughter cells. LocZ has a unique localization profile. It arrives early at midcell, before FtsZ and FtsA, and leaves the septum early, apparently moving along with the equatorial rings that mark the future division sites. Consistently, cells lacking LocZ also show misplacement of the Z-ring, suggesting that it could act as a positive regulator to determine septum placement. LocZ was identified as a substrate of the Ser/Thr protein kinase StkP, which regulates cell division in S. pneumoniae. Interestingly, homologues of LocZ are found only in streptococci, lactococci, and enterococci, indicating that this close phylogenetically related group of bacteria evolved a specific solution to spatially regulate cell division. PMID:25550321

Timing of Tissue-specific Cell Division Requires a Differential Onset of Zygotic Transcription during Metazoan Embryogenesis*

PubMed Central

Wong, Ming-Kin; Guan, Daogang; Ng, Kaoru Hon Chun; Ho, Vincy Wing Sze; An, Xiaomeng; Li, Runsheng; Ren, Xiaoliang

2016-01-01

Metazoan development demands not only precise cell fate differentiation but also accurate timing of cell division to ensure proper development. How cell divisions are temporally coordinated during development is poorly understood. Caenorhabditis elegans embryogenesis provides an excellent opportunity to study this coordination due to its invariant development and widespread division asynchronies. One of the most pronounced asynchronies is a significant delay of cell division in two endoderm progenitor cells, Ea and Ep, hereafter referred to as E2, relative to its cousins that mainly develop into mesoderm organs and tissues. To unravel the genetic control over the endoderm-specific E2 division timing, a total of 822 essential and conserved genes were knocked down using RNAi followed by quantification of cell cycle lengths using in toto imaging of C. elegans embryogenesis and automated lineage. Intriguingly, knockdown of numerous genes encoding the components of general transcription pathway or its regulatory factors leads to a significant reduction in the E2 cell cycle length but an increase in cell cycle length of the remaining cells, indicating a differential requirement of transcription for division timing between the two. Analysis of lineage-specific RNA-seq data demonstrates an earlier onset of transcription in endoderm than in other germ layers, the timing of which coincides with the birth of E2, supporting the notion that the endoderm-specific delay in E2 division timing demands robust zygotic transcription. The reduction in E2 cell cycle length is frequently associated with cell migration defect and gastrulation failure. The results suggest that a tissue-specific transcriptional activation is required to coordinate fate differentiation, division timing, and cell migration to ensure proper development. PMID:27056332

76 FR 16446 - Delphi Corporation Electronics And Safety Division Including On-Site Leased Workers From Acro...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-23

...: Heating, ventilating, air-conditioning systems (HVAC), amplifiers, mainboards, gas control modules, hybrid airmeter electronics, hybrid ignition electronics, pressure sensors, transmission control modules, crash...

Temporal controls of the asymmetric cell division cycle in Caulobacter crescentus.

PubMed

Li, Shenghua; Brazhnik, Paul; Sobral, Bruno; Tyson, John J

2009-08-01

The asymmetric cell division cycle of Caulobacter crescentus is orchestrated by an elaborate gene-protein regulatory network, centered on three major control proteins, DnaA, GcrA and CtrA. The regulatory network is cast into a quantitative computational model to investigate in a systematic fashion how these three proteins control the relevant genetic, biochemical and physiological properties of proliferating bacteria. Different controls for both swarmer and stalked cell cycles are represented in the mathematical scheme. The model is validated against observed phenotypes of wild-type cells and relevant mutants, and it predicts the phenotypes of novel mutants and of known mutants under novel experimental conditions. Because the cell cycle control proteins of Caulobacter are conserved across many species of alpha-proteobacteria, the model we are proposing here may be applicable to other genera of importance to agriculture and medicine (e.g., Rhizobium, Brucella).

RESEARCH & DEVELOPMENT OF PREVENTION AND CONTROL MEASURES FOR MOLD CONTAMINATION

EPA Science Inventory

The U.S. Environmental Protection Agency, Air Pollution Prevention and Control Division, Indoor Environment Management Branch has, since 1995, conducted research into controlling biological contamination in the indoor environment. In this paper four areas of research are discusse...

Bibliography Section. Bibliographic Control Division. Papers.

ERIC Educational Resources Information Center

International Federation of Library Associations, The Hague (Netherlands).

Papers on national bibliographies and bibliographic control, which were presented at the 1983 International Federation of Library Associations (IFLA) conference, include: (1) "Bibliographic Control of IFLA Publications: Retrospective and Current" by Mona Madsen (Denmark), which outlines types of IFLA documents, existing bibliographies,…

40 CFR 60.4 - Address.

Code of Federal Regulations, 2012 CFR

2012-07-01

.... Environmental Protection Agency, 1200 Sixth Avenue, Seattle, WA 98101. (b) Section 111(c) directs the..., Division of Air Pollution Control, Arkansas Department of Pollution Control and Ecology, 8001 National... Park Avenue, Victorville, CA 92392-2310. Monterey Bay Unified Air Pollution Control District, 24580...

Dynamic bandwidth allocation based on multiservice in software-defined wavelength-division multiplexing time-division multiplexing passive optical network

NASA Astrophysics Data System (ADS)

Wang, Fu; Liu, Bo; Zhang, Lijia; Jin, Feifei; Zhang, Qi; Tian, Qinghua; Tian, Feng; Rao, Lan; Xin, Xiangjun

2017-03-01

The wavelength-division multiplexing passive optical network (WDM-PON) is a potential technology to carry multiple services in an optical access network. However, it has the disadvantages of high cost and an immature technique for users. A software-defined WDM/time-division multiplexing PON was proposed to meet the requirements of high bandwidth, high performance, and multiple services. A reasonable and effective uplink dynamic bandwidth allocation algorithm was proposed. A controller with dynamic wavelength and slot assignment was introduced, and a different optical dynamic bandwidth management strategy was formulated flexibly for services of different priorities according to the network loading. The simulation compares the proposed algorithm with the interleaved polling with adaptive cycle time algorithm. The algorithm shows better performance in average delay, throughput, and bandwidth utilization. The results show that the delay is reduced to 62% and the throughput is improved by 35%.

The Division of Household Labor: Longitudinal Changes and Within-Couple Variation.

PubMed

Lam, Chun Bun; McHale, Susan M; Crouter, Ann C

2012-10-01

This study examined how the division of household labor changed as a function of marital duration and whether within-couple variation in spouses' relative power and availability were linked to within-couple variation in the division of labor. On 4 occasions over 7 years, 188 stably married couples reported on their housework activities using daily diaries. Multilevel models revealed that wives' portions of household responsibilities declined over time, and that changes in spouses' relative income and work hours were linked to changes in housework allocation. Wives with husbands who perceived greater marital control, on average, did proportionally more housework, and for couples with husbands who had highly autonomous jobs, changes in spouses' relative psychological job involvement were linked to changes in housework allocation. Findings highlight the importance of understanding household division of labor as a lifespan phenomenon, the distinction between within- versus between-couple associations, and the multidimensional nature of power and availability.

Spire, an actin nucleation factor, regulates cell division during Drosophila heart development.

PubMed

Xu, Peng; Johnson, Tamara L; Stoller-Conrad, Jessica R; Schulz, Robert A

2012-01-01

The Drosophila dorsal vessel is a beneficial model system for studying the regulation of early heart development. Spire (Spir), an actin-nucleation factor, regulates actin dynamics in many developmental processes, such as cell shape determination, intracellular transport, and locomotion. Through protein expression pattern analysis, we demonstrate that the absence of spir function affects cell division in Myocyte enhancer factor 2-, Tinman (Tin)-, Even-skipped- and Seven up (Svp)-positive heart cells. In addition, genetic interaction analysis shows that spir functionally interacts with Dorsocross, tin, and pannier to properly specify the cardiac fate. Furthermore, through visualization of double heterozygous embryos, we determines that spir cooperates with CycA for heart cell specification and division. Finally, when comparing the spir mutant phenotype with that of a CycA mutant, the results suggest that most Svp-positive progenitors in spir mutant embryos cannot undergo full cell division at cell cycle 15, and that Tin-positive progenitors are arrested at cell cycle 16 as double-nucleated cells. We conclude that Spir plays a crucial role in controlling dorsal vessel formation and has a function in cell division during heart tube morphogenesis.

Division rate, cell size and proteome allocation: impact on gene expression noise and implications for the dynamics of genetic circuits

PubMed Central

2018-01-01

The cell division rate, size and gene expression programmes change in response to external conditions. These global changes impact on average concentrations of biomolecule and their variability or noise. Gene expression is inherently stochastic, and noise levels of individual proteins depend on synthesis and degradation rates as well as on cell-cycle dynamics. We have modelled stochastic gene expression inside growing and dividing cells to study the effect of division rates on noise in mRNA and protein expression. We use assumptions and parameters relevant to Escherichia coli, for which abundant quantitative data are available. We find that coupling of transcription, but not translation rates to the rate of cell division can result in protein concentration and noise homeostasis across conditions. Interestingly, we find that the increased cell size at fast division rates, observed in E. coli and other unicellular organisms, buffers noise levels even for proteins with decreased expression at faster growth. We then investigate the functional importance of these regulations using gene regulatory networks that exhibit bi-stability and oscillations. We find that network topology affects robustness to changes in division rate in complex and unexpected ways. In particular, a simple model of persistence, based on global physiological feedback, predicts increased proportion of persister cells at slow division rates. Altogether, our study reveals how cell size regulation in response to cell division rate could help controlling gene expression noise. It also highlights that understanding circuits' robustness across growth conditions is key for the effective design of synthetic biological systems. PMID:29657814

Symmetry of initial cell divisions among primitive hematopoietic progenitors is independent of ontogenic age and regulatory molecules.

PubMed

Huang, S; Law, P; Francis, K; Palsson, B O; Ho, A D

1999-10-15

We have developed a time-lapse camera system to follow the replication history and the fate of hematopoietic stem cells (HSC) at a single-cell level. Combined with single-cell culture, we correlated the early replication behavior with colony development after 14 days. The membrane dye PKH26 was used to monitor cell division. In addition to multiple, synchronous, and symmetric divisions, single-sorted CD34(+)/CD38(-) cells derived from fetal liver (FLV) also gave rise to a daughter cell that remained quiescent for up to 8 days, whereas the other daughter cell proliferated exponentially. Upon separation and replating as single cells onto medium containing a cytokine cocktail, 60.6% +/- 9.8% of the initially quiescent cells (PKH26 bright) gave rise again to colonies and 15.8% +/- 7.8% to blast colonies that could be replated. We have then determined the effects of various regulatory molecules on symmetry of initial cell divisions. After single-cell sorting, the CD34(+)/CD38(-) cells derived from FLV were exposed to flt3-ligand, thrombopoietin, stem cell factor (SCF), or medium containing a cytokine cocktail (with SCF, interleukin-3, interleukin-6, granulocyte-macrophage colony-stimulating factor, and erythropoietin). Whereas mitotic rate, colony efficiency, and asymmetric divisions could be altered using various regulatory molecules, the asymmetric division index, defined as the number of asymmetric divisions versus the number of dividing cells, was not altered significantly. This observation suggests that, although lineage commitment and cell proliferation can be skewed by extrinsic signaling, symmetry of early divisions is probably under the control of intrinsic factors.

System access control study

DOT National Transportation Integrated Search

1974-06-01

The report presents a summary of a study conducted for the Transportation Systems Center of promising access control techniques which are applicable to an aeronautical satellite system. Several frequency division multiple access (FDMA) and time divis...

Visible-Light Resin Curing Units.

DTIC Science & Technology

1984-03-01

dental unit’s pilot or drive air pressure, and is activated via the foot control that also operates the high-speed and low-speed handpieces . For all...the Dental Investigation Sqtc* kvmtc)b Cini’cal Sciences Division, USAF School of Aerospace 114,~e Ameoae ~dical Division, APSC, Brooks Air force... Dental curing units White light activation II Visible-light activation 19. AISTRACT (Continue on ,werse if necenawy and identify by

Assessing the use of Low Voltage UV-light Emitting Miniature LEDs for Marine Biofouling Control

DTIC Science & Technology

2016-07-01

Piola, 1Bart Salters, Clare Grandison, Mark Ciacic and 1Roelant Hietbrink Maritime Division Defence Science and Technology Group 1Philips...uniformly all over the coating layer. In this report, we present a description of the technology and experimental setup, as well as the results of...UNCLASSIFIED Published by Maritime Division Defence Science and Technology Group 506 Lorimer St Fishermans Bend, Victoria 3207 Australia

Homeostasis 5: nurses as external agents of control in breast cancer.

PubMed

Clancy, John; McVicar, Andrew

Breast cancer is caused by a homeostatic imbalance of cell division. Healthcare practitioners need to understand cellular activities to appreciate the physiological basis of health (homeostasis), the pathophysiological basis of illness and the physiological rationale of healthcare. Cells are the 'basic unit of life' (Clancy and McVicar, 2011a). This article describes normal cell division and the anatomy and physiology of the breast and, using a case study, will show how breast cancer is a homeostatic imbalance of cell division. There are analogies between the components of homeostasis and the components of the nursing (healthcare) process (Clancy and McVicar, 2011b) in the condition of breast cancer. After reading this article, nurses should be able to: understand that breast cancer is a cellular hence chemical imbalance that causes uncontrollable mitotic division of breast cells; understand how the cell cycle of cancer cells differs from that of normal cells; identify nature-nurture interactions involved in the aetiology of breast cancer; understand that when caring for people with breast cancer, health professionals including oncology nurses are acting as external agents of homeostatic control as the patient 'recovers' from breast cancer, and also to some extent when reducing signs and symptoms, hence quality of life, by providing palliative care.

LAM-1 and FAT Genes Control Development of the Leaf Blade in Nicotiana sylvestris.

PubMed Central

McHale, NA

1993-01-01

Leaf primordia of the lam-1 mutant of Nicotiana sylvestris grow normally in length but remain bladeless throughout development. The blade initiation site is established at the normal time and position in lam-1 primordia. Anticlinal divisions proceed normally in the outer L1 and L2 layers, but the inner L3 cells fail to establish the periclinal divisions that normally generate the middle mesophyll core. The lam-1 mutation also blocks formation of blade mesophyll from distal L2 cells. This suggests that LAM-1 controls a common step in initiation of blade tissue from the L2 and L3 lineage of the primordium. Another recessive mutation (fat) was isolated in N. sylvestris that induces abnormal periclinal divisions in the mesophyll during blade initiation and expansion. This generates a blade approximately twice its normal thickness by doubling the number of mesophyll cell layers from four to approximately eight. Presumably, the fat mutation defines a negative regulator involved in repression of periclinal divisions in the blade. The lam-1 fat double mutant shows radial proliferation of mesophyll cells at the blade initiation site. This produces a highly disorganized, club-shaped blade that appears to represent an additive effect of the lam-1 and fat mutations on blade founder cells. PMID:12271096

Arabidopsis JACKDAW and MAGPIE zinc finger proteins delimit asymmetric cell division and stabilize tissue boundaries by restricting SHORT-ROOT action

PubMed Central

Welch, David; Hassan, Hala; Blilou, Ikram; Immink, Richard; Heidstra, Renze; Scheres, Ben

2007-01-01

In the Arabidopsis root, the SHORT-ROOT transcription factor moves outward to the ground tissue from its site of transcription in the stele and is required for the specification of the endodermis and the stem cell organizing quiescent center cells. In addition, SHORT-ROOT and the downstream transcription factor SCARECROW control an oriented cell division in ground tissue stem cell daughters. Here, we show that the JACKDAW and MAGPIE genes, which encode members of a plant-specific family of zinc finger proteins, act in a SHR-dependent feed-forward loop to regulate the range of action of SHORT-ROOT and SCARECROW. JACKDAW expression is initiated independent of SHORT-ROOT and regulates the SCARECROW expression domain outside the stele, while MAGPIE expression depends on SHORT-ROOT and SCARECROW. We provide evidence that JACKDAW and MAGPIE regulate tissue boundaries and asymmetric cell division and can control SHORT-ROOT and SCARECROW activity in a transcriptional and protein interaction network. PMID:17785527

Marginalization in neural circuits with divisive normalization

PubMed Central

Beck, J.M.; Latham, P.E.; Pouget, A.

2011-01-01

A wide range of computations performed by the nervous system involves a type of probabilistic inference known as marginalization. This computation comes up in seemingly unrelated tasks, including causal reasoning, odor recognition, motor control, visual tracking, coordinate transformations, visual search, decision making, and object recognition, to name just a few. The question we address here is: how could neural circuits implement such marginalizations? We show that when spike trains exhibit a particular type of statistics – associated with constant Fano factors and gain-invariant tuning curves, as is often reported in vivo – some of the more common marginalizations can be achieved with networks that implement a quadratic nonlinearity and divisive normalization, the latter being a type of nonlinear lateral inhibition that has been widely reported in neural circuits. Previous studies have implicated divisive normalization in contrast gain control and attentional modulation. Our results raise the possibility that it is involved in yet another, highly critical, computation: near optimal marginalization in a remarkably wide range of tasks. PMID:22031877

Parental age and the origin of trisomy 21. A study of 302 families.

PubMed

Dagna Bricarelli, F; Pierluigi, M; Landucci, M; Arslanian, A; Coviello, D A; Ferro, M A; Strigini, P

1989-04-01

Several studies have attempted to define the role of parental age in determining the prevalence of 47, +21 according to the origin of nondisjunction. This report analyzes the original data of 197 informative families from Italy and reviews the available literature (96 families from Denmark and 201 from other countries). Mothers whose gametes showed nondisjunction are treated as cases, and those with normal meiosis as controls within each study. To utilize the data fully, maternal age at birth of a 47, +21 individual is treated as a continuous variable in a nonparametric comparison. The combined evidence indicates that nondisjunction in the female is associated with a significant age difference between cases and controls which is mostly due to errors in the second meiotic division. It may be inferred that in the general population, aging enhances nondisjunction at both first and second division in the female, while aging in the male is presumably associated mostly (or only) with first division errors. Implications and alternative models are discussed.

Perceived facial changes of Class II Division 1 patients with convex profiles after functional orthopedic treatment followed by fixed orthodontic appliances.

PubMed

Tsiouli, Kleopatra; Topouzelis, Nikolaos; Papadopoulos, Moschos A; Gkantidis, Nikolaos

2017-07-01

The aim of this research was to investigate the perceived facial changes in Class II Division 1 patients with convex profiles after functional orthopedic treatment followed by fixed orthodontic appliances. Pretreatment and posttreatment profile photographs of 12 Class II Division 1 patients treated with activators, 12 Class II Division 1 patients treated with Twin-block appliances, and 12 controls with normal profiles treated without functional appliances were presented in pairs to 10 orthodontists, 10 patients, 10 parents, and 10 laypersons. The raters assessed changes in facial appearance on a visual analog scale. Two-way multivariate analysis of variance was used to evaluate differences among group ratings. Intrarater reliability was strong in most cases (intraclass correlation coefficients, >0.7). The internal consistency of the assessments was high (alpha, >0.87), both within and between groups. The raters consistently perceived more positive changes in the Class II Division 1 groups compared with the control group. However, this difference hardly exceeded 1/10th of the total visual analog scale length in its highest value and was mostly evident in the lower face and chin. No significant differences were found between the activator and the Twin-block groups. Although the raters perceived improvements of the facial profiles after functional orthopedic treatment followed by fixed orthodontic appliances, these were quite limited. Thus, orthodontists should be tentative when predicting significant improvement of a patient's profile with this treatment option. Copyright © 2017 American Association of Orthodontists. Published by Elsevier Inc. All rights reserved.

Activation of Meiosis-Specific Genes is Associated with Depolyploidization of Human Tumor Cells Following Radiation-Induced Mitotic Catastrophe

PubMed Central

Ianzini, Fiorenza; Kosmacek, Elizabeth A.; Nelson, Elke S.; Napoli, Eleonora; Erenpreisa, Jekaterina; Kalejs, Martins; Mackey, Michael A.

2009-01-01

Cancer is frequently characterized histologically by the appearance of large cells that are either aneuploid or polyploid. Aneuploidy and polyploidy are hallmarks of radiation-induced mitotic catastrophe (MC), a common phenomenon occurring in tumor cells with impaired p53 function exposed to various cytotoxic and genotoxic agents. MC is characterized by altered expression of mitotic regulators, untimely and abnormal cell division, delayed DNA damage, and changes in morphology. We report here that cells undergoing radiation-induced MC are more plastic with regards to ploidy and that this plasticity allows them to reorganize their genetic material through reduction divisions to produce smaller cells morphologically indistinguishable from control cells. Experiments conducted with the Large Scale Digital Cell Analysis System (LSDCAS) are discussed that show that a small fraction of polyploid cancer cells formed via radiation-induced MC can survive and start a process of depolyploidization that yields various outcomes. While most multipolar divisions failed and cell fusion occurred; some of these divisions were successful and originated a variety of cell progeny characterized by different ploidy. Among these ploidy phenotypes, a progeny of small mononucleated cells, indistinguishable from the untreated control cells, is often seen. We report here evidence that meiosis-specific genes are expressed in the polyploid cells during depolyploidization. Tumor cells might take advantage of the temporary change from a pro-mitotic to a pro-meiotic division regimen to facilitate depolyploidization and restore the proliferative state of the tumor cell population. These events might be mechanisms by which tumor progression and resistance to treatment occur in vivo. PMID:19258501

Analysis of Noise Mechanisms in Cell-Size Control.

PubMed

Modi, Saurabh; Vargas-Garcia, Cesar Augusto; Ghusinga, Khem Raj; Singh, Abhyudai

2017-06-06

At the single-cell level, noise arises from multiple sources, such as inherent stochasticity of biomolecular processes, random partitioning of resources at division, and fluctuations in cellular growth rates. How these diverse noise mechanisms combine to drive variations in cell size within an isoclonal population is not well understood. Here, we investigate the contributions of different noise sources in well-known paradigms of cell-size control, such as adder (division occurs after adding a fixed size from birth), sizer (division occurs after reaching a size threshold), and timer (division occurs after a fixed time from birth). Analysis reveals that variation in cell size is most sensitive to errors in partitioning of volume among daughter cells, and not surprisingly, this process is well regulated among microbes. Moreover, depending on the dominant noise mechanism, different size-control strategies (or a combination of them) provide efficient buffering of size variations. We further explore mixer models of size control, where a timer phase precedes/follows an adder, as has been proposed in Caulobacter crescentus. Although mixing a timer and an adder can sometimes attenuate size variations, it invariably leads to higher-order moments growing unboundedly over time. This results in a power-law distribution for the cell size, with an exponent that depends inversely on the noise in the timer phase. Consistent with theory, we find evidence of power-law statistics in the tail of C. crescentus cell-size distribution, although there is a discrepancy between the observed power-law exponent and that predicted from the noise parameters. The discrepancy, however, is removed after data reveal that the size added by individual newborns in the adder phase itself exhibits power-law statistics. Taken together, this study provides key insights into the role of noise mechanisms in size homeostasis, and suggests an inextricable link between timer-based models of size control and heavy-tailed cell-size distributions. Copyright © 2017 Biophysical Society. Published by Elsevier Inc. All rights reserved.

Respiratory Syncytial Virus Infection (RSV): Transmission and Prevention

MedlinePlus

... of Search Controls Search Form Controls Cancel Submit Respiratory Syncytial Virus Infection (RSV) Note: Javascript is disabled ... 2018 Content source: National Center for Immunization and Respiratory Diseases (NCIRD) , Division of Viral Diseases Email Recommend ...

Environment, Safety, and Health Self-Assessment Report, Fiscal Year 2008

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chernowski, John

2009-02-27

Lawrence Berkeley National Laboratory's Environment, Safety, and Health (ES&H) Self-Assessment Program ensures that Integrated Safety Management (ISM) is implemented institutionally and by all divisions. The Self-Assessment Program, managed by the Office of Contract Assurance (OCA), provides for an internal evaluation of all ES&H programs and systems at LBNL. The functions of the program are to ensure that work is conducted safely, and with minimal negative impact to workers, the public, and the environment. The Self-Assessment Program is also the mechanism used to institute continuous improvements to the Laboratory's ES&H programs. The program is described in LBNL/PUB 5344, Environment, Safety, andmore » Health Self-Assessment Program and is composed of four distinct assessments: the Division Self-Assessment, the Management of Environment, Safety, and Health (MESH) review, ES&H Technical Assurance, and the Appendix B Self-Assessment. The Division Self-Assessment uses the five core functions and seven guiding principles of ISM as the basis of evaluation. Metrics are created to measure performance in fulfilling ISM core functions and guiding principles, as well as promoting compliance with applicable regulations. The five core functions of ISM are as follows: (1) Define the Scope of Work; (2) Identify and Analyze Hazards; (3) Control the Hazards; (4) Perform the Work; and (5) Feedback and Improvement. The seven guiding principles of ISM are as follows: (1) Line Management Responsibility for ES&H; (2) Clear Roles and Responsibilities; (3) Competence Commensurate with Responsibilities; (4) Balanced Priorities; (5) Identification of ES&H Standards and Requirements; (6) Hazard Controls Tailored to the Work Performed; and (7) Operations Authorization. Performance indicators are developed by consensus with OCA, representatives from each division, and Environment, Health, and Safety (EH&S) Division program managers. Line management of each division performs the Division Self-Assessment annually. The primary focus of the review is workplace safety. The MESH review is an evaluation of division management of ES&H in its research and operations, focusing on implementation and effectiveness of the division's ISM plan. It is a peer review performed by members of the LBNL Safety Review Committee (SRC), with staff support from OCA. Each division receives a MESH review every two to four years, depending on the results of the previous review. The ES&H Technical Assurance Program (TAP) provides the framework for systematic reviews of ES&H programs and processes. The intent of ES&H Technical Assurance assessments is to provide assurance that ES&H programs and processes comply with their guiding regulations, are effective, and are properly implemented by LBNL divisions. The Appendix B Performance Evaluation and Measurement Plan (PEMP) requires that LBNL sustain and enhance the effectiveness of integrated safety, health, and environmental protection through a strong and well-deployed system. Information required for Appendix B is provided by EH&S Division functional managers. The annual Appendix B report is submitted at the close of the fiscal year. This assessment is the Department of Energy's (DOE) primary mechanism for evaluating LBNL's contract performance in ISM.« less

LexA Binds to Transcription Regulatory Site of Cell Division Gene ftsZ in Toxic Cyanobacterium Microcystis aeruginosa.

PubMed

Honda, Takashi; Morimoto, Daichi; Sako, Yoshihiko; Yoshida, Takashi

2018-05-17

Previously, we showed that DNA replication and cell division in toxic cyanobacterium Microcystis aeruginosa are coordinated by transcriptional regulation of cell division gene ftsZ and that an unknown protein specifically bound upstream of ftsZ (BpFz; DNA-binding protein to an upstream site of ftsZ) during successful DNA replication and cell division. Here, we purified BpFz from M. aeruginosa strain NIES-298 using DNA-affinity chromatography and gel-slicing combined with gel electrophoresis mobility shift assay (EMSA). The N-terminal amino acid sequence of BpFz was identified as TNLESLTQ, which was identical to that of transcription repressor LexA from NIES-843. EMSA analysis using mutant probes showed that the sequence GTACTAN 3 GTGTTC was important in LexA binding. Comparison of the upstream regions of lexA in the genomes of closely related cyanobacteria suggested that the sequence TASTRNNNNTGTWC could be a putative LexA recognition sequence (LexA box). Searches for TASTRNNNNTGTWC as a transcriptional regulatory site (TRS) in the genome of M. aeruginosa NIES-843 showed that it was present in genes involved in cell division, photosynthesis, and extracellular polysaccharide biosynthesis. Considering that BpFz binds to the TRS of ftsZ during normal cell division, LexA may function as a transcriptional activator of genes related to cell reproduction in M. aeruginosa, including ftsZ. This may be an example of informality in the control of bacterial cell division.

Cytokinesis-Based Constraints on Polarized Cell Growth in Fission Yeast

PubMed Central

Bohnert, K. Adam; Gould, Kathleen L.

2012-01-01

The rod-shaped fission yeast Schizosaccharomyces pombe, which undergoes cycles of monopolar-to-bipolar tip growth, is an attractive organism for studying cell-cycle regulation of polarity establishment. While previous research has described factors mediating this process from interphase cell tips, we found that division site signaling also impacts the re-establishment of bipolar cell growth in the ensuing cell cycle. Complete loss or targeted disruption of the non-essential cytokinesis protein Fic1 at the division site, but not at interphase cell tips, resulted in many cells failing to grow at new ends created by cell division. This appeared due to faulty disassembly and abnormal persistence of the cell division machinery at new ends of fic1Δ cells. Moreover, additional mutants defective in the final stages of cytokinesis exhibited analogous growth polarity defects, supporting that robust completion of cell division contributes to new end-growth competency. To test this model, we genetically manipulated S. pombe cells to undergo new end take-off immediately after cell division. Intriguingly, such cells elongated constitutively at new ends unless cytokinesis was perturbed. Thus, cell division imposes constraints that partially override positive controls on growth. We posit that such constraints facilitate invasive fungal growth, as cytokinesis mutants displaying bipolar growth defects formed numerous pseudohyphae. Collectively, these data highlight a role for previous cell cycles in defining a cell's capacity to polarize at specific sites, and they additionally provide insight into how a unicellular yeast can transition into a quasi-multicellular state. PMID:23093943

Divisions of general practice in Australia: how do they measure up in the international context?

PubMed Central

Smith, Judith; Sibthorpe, Beverly

2007-01-01

Background Since the late 1980s, there has been evidence of an international trend towards more organised primary care. This has taken a number of forms including the emergence of primary care organisations. Underpinning such developments is an inherent belief in evidence that suggests that well-developed primary care is associated with improved health outcomes and greater cost-effectiveness within health systems. In Australia, primary care organisations have emerged as divisions of general practice. These are professionally-led, regionally-based, and largely government-funded voluntary associations of general practitioners that seek to co-ordinate local primary care services, and improve the quality of care and health outcomes for local communities. Discussion In this paper, we examine and debate the development of divisions in the international context, using six roles of primary care organisations outlined in published research. The six roles that are used as the basis for the critique are the ability of primary care organisations to: improve health outcomes; manage demand and control costs; engage primary care physicians; enable greater integration of health services; develop more accessible services in community and primary care settings; and enable greater scrutiny and assurance of quality of primary care services. Summary We conclude that there has been an evolutionary approach to divisions' development and they now appear embedded as geographically-based planning and development organisations within the Australian primary health care system. The Australian Government has to date been cautious in its approach to intervention in divisions' direction and performance. However, options for the next phase include: making greater use of contracts between government and divisions; introducing and extending proposed national quality targets for divisions, linked with financial or other incentives for performance; government sub-contracting with state-based organisations to act as purchasers of care; pursuing a fund-holding approach within divisions; and developing divisions as a form of health maintenance organisation. The challenge for the Australian Government, should it wish to see divisions' role expand, is to find mechanisms to enable this without compromising the relatively strong GP engagement that increasingly distinguishes divisions of general practice within the international experience of primary care organisations. PMID:17626642

A central mesencephalic reticular formation projection to the Edinger-Westphal nuclei.

PubMed

May, Paul J; Warren, Susan; Bohlen, Martin O; Barnerssoi, Miriam; Horn, Anja K E

2016-11-01

The central mesencephalic reticular formation, a region associated with horizontal gaze control, has recently been shown to project to the supraoculomotor area in primates. The Edinger-Westphal nucleus is found within the supraoculomotor area. It has two functionally and anatomically distinct divisions: (1) the preganglionic division, which contains motoneurons that control both the actions of the ciliary muscle, which focuses the lens, and the sphincter pupillae muscle, which constricts the iris, and (2) the centrally projecting division, which contains peptidergic neurons that play a role in food and fluid intake, and in stress responses. In this study, we used neuroanatomical tracers in conjunction with immunohistochemistry in Macaca fascicularis monkeys to examine whether either of these Edinger-Westphal divisions receives synaptic input from the central mesencephalic reticular formation. Anterogradely labeled reticular axons were observed making numerous boutonal associations with the cholinergic, preganglionic motoneurons of the Edinger-Westphal nucleus. These associations were confirmed to be synaptic contacts through the use of confocal and electron microscopic analysis. The latter indicated that these terminals generally contained pleomorphic vesicles and displayed symmetric, synaptic densities. Examination of urocortin-1-positive cells in the same cases revealed fewer examples of unambiguous synaptic relationships, suggesting the centrally projecting Edinger-Westphal nucleus is not the primary target of the projection from the central mesencephalic reticular formation. We conclude from these data that the central mesencephalic reticular formation must play a here-to-for unexpected role in control of the near triad (vergence, lens accommodation and pupillary constriction), which is used to examine objects in near space.

A central mesencephalic reticular formation projection to the Edinger–Westphal nuclei

PubMed Central

May, Paul J.; Warren, Susan; Bohlen, Martin O.; Barnerssoi, Miriam

2016-01-01

The central mesencephalic reticular formation, a region associated with horizontal gaze control, has recently been shown to project to the supraoculomotor area in primates. The Edinger–Westphal nucleus is found within the supraoculomotor area. It has two functionally and anatomically distinct divisions: (1) the preganglionic division, which contains motoneurons that control both the actions of the ciliary muscle, which focuses the lens, and the sphincter pupillae muscle, which constricts the iris, and (2) the centrally projecting division, which contains peptidergic neurons that play a role in food and fluid intake, and in stress responses. In this study, we used neuroanatomical tracers in conjunction with immunohistochemistry in Macaca fascicularis monkeys to examine whether either of these Edinger–Westphal divisions receives synaptic input from the central mesencephalic reticular formation. Anterogradely labeled reticular axons were observed making numerous boutonal associations with the cholinergic, preganglionic motoneurons of the Edinger–Westphal nucleus. These associations were confirmed to be synaptic contacts through the use of confocal and electron microscopic analysis. The latter indicated that these terminals generally contained pleomorphic vesicles and displayed symmetric, synaptic densities. Examination of urocortin-1-positive cells in the same cases revealed fewer examples of unambiguous synaptic relationships, suggesting the centrally projecting Edinger–Westphal nucleus is not the primary target of the projection from the central mesencephalic reticular formation. We conclude from these data that the central mesencephalic reticular formation must play a here-to-for unexpected role in control of the near triad (vergence, lens accommodation and pupillary constriction), which is used to examine objects in near space. PMID:26615603

Hyperthyroidism Due to a Thyrotropin Secreting Pituitary Adenoma: Studies of Thyrotropin and Subunit Secretion

DTIC Science & Technology

1982-03-10

ADDRESS 10. PROGRAM ELEMENT. PROJECT. TASK Division of Medicine , Walter Reed Army Institute o7 AREA & WORK UNIT NUMBERS Research, Endocrine Metabolic...Service, Walter Reed C Army Medical Center, Washington, D.C. 20012 I. CONTROLLING OFFICE NAME AND ADDRESS 12. REPORT DATE Division of Medicine Walter...THIS PAGE (When Date Entered) * .1 Unclassified SECURITY CLASSIFICATION OF THIS PAGIE(When "ea Entmuod)- antithyroid drug therapy. Estrogens produced a

Air Force Roles and Missions: A History

DTIC Science & Technology

1998-01-01

armies fighting on the Western Front. In July, Foch gave Pershing tactical control of a sector in the Chateau- Thierry region, with only one division...In the battle at Chateau- Thierry in July, air squadrons flying support for the American division learned a costly lesson. Air units were deci- mated... Thierry , he saw Trenchard’s views on the need to concentrate air forces to achieve aerial superiority confirmed. The losses helped Mitchell convince

Reformulation of the CBR Procedure. Report 1: Basic Report

DTIC Science & Technology

2012-04-01

originate a superior method of compaction control, the modified density test associated with the name of Ralph R. Proctor . In time, Porter was able to...enough for the subsoil moisture to equalize. Colonel Bragdon in the South Atlantic Division was to choose an airstrip built on sandy clay , a fairly good...subsoil; Colonel Scott in the Southwestern Division, one of lean black clay , a rather poor foundation; Colonel Elliott in the Upper Mississippi

Genetic Dissection of DivIVA Functions in Listeria monocytogenes.

PubMed

Kaval, Karan Gautam; Hauf, Samuel; Rismondo, Jeanine; Hahn, Birgitt; Halbedel, Sven

2017-12-15

DivIVA is a membrane binding protein that clusters at curved membrane regions, such as the cell poles and the membrane invaginations occurring during cell division. DivIVA proteins recruit many other proteins to these subcellular sites through direct protein-protein interactions. DivIVA-dependent functions are typically associated with cell growth and division, even though species-specific differences in the spectrum of DivIVA functions and their causative interaction partners exist. DivIVA from the Gram-positive human pathogen Listeria monocytogenes has at least three different functions. In this bacterium, DivIVA is required for precise positioning of the septum at midcell, it contributes to the secretion of autolysins required for the breakdown of peptidoglycan at the septum after the completion of cell division, and it is essential for flagellar motility. While the DivIVA interaction partners for control of division site selection are well established, the proteins connecting DivIVA with autolysin secretion or swarming motility are completely unknown. We set out to identify divIVA alleles in which these three DivIVA functions could be separated, since the question of the degree to which the three functions of L. monocytogenes DivIVA are interlinked could not be answered before. Here, we identify such alleles, and our results show that division site selection, autolysin secretion, and swarming represent three discrete pathways that are independently influenced by DivIVA. These findings provide the required basis for the identification of DivIVA interaction partners controlling autolysin secretion and swarming in the future. IMPORTANCE DivIVA of the pathogenic bacterium Listeria monocytogenes is a central scaffold protein that influences at least three different cellular processes, namely, cell division, protein secretion, and bacterial motility. How DivIVA coordinates these rather unrelated processes is not known. We here identify variants of L. monocytogenes DivIVA, in which these functions are separated from each other. These results have important implications for the models explaining how DivIVA interacts with other proteins. Copyright © 2017 American Society for Microbiology.

Green Retrofits to Bring Jobs, Stormwater Controls

EPA Pesticide Factsheets

Community-based public-private partnership fostered by EPA’s Mid-Atlantic Water Protection Division is underway in Prince George’s County, Maryland, to generate “faster, cheaper, greener” controls for stormwater and benefit the local economy and community.

Energy-efficient orthogonal frequency division multiplexing-based passive optical network based on adaptive sleep-mode control and dynamic bandwidth allocation

NASA Astrophysics Data System (ADS)

Zhang, Chongfu; Xiao, Nengwu; Chen, Chen; Yuan, Weicheng; Qiu, Kun

2016-02-01

We propose an energy-efficient orthogonal frequency division multiplexing-based passive optical network (OFDM-PON) using adaptive sleep-mode control and dynamic bandwidth allocation. In this scheme, a bidirectional-centralized algorithm named the receiver and transmitter accurate sleep control and dynamic bandwidth allocation (RTASC-DBA), which has an overall bandwidth scheduling policy, is employed to enhance the energy efficiency of the OFDM-PON. The RTASC-DBA algorithm is used in an optical line terminal (OLT) to control the sleep mode of an optical network unit (ONU) sleep and guarantee the quality of service of different services of the OFDM-PON. The obtained results show that, by using the proposed scheme, the average power consumption of the ONU is reduced by ˜40% when the normalized ONU load is less than 80%, compared with the average power consumption without using the proposed scheme.

Characterization of dependencies between growth and division in budding yeast.

PubMed

Mayhew, Michael B; Iversen, Edwin S; Hartemink, Alexander J

2017-02-01

Cell growth and division are processes vital to the proliferation and development of life. Coordination between these two processes has been recognized for decades in a variety of organisms. In the budding yeast Saccharomyces cerevisiae , this coordination or 'size control' appears as an inverse correlation between cell size and the rate of cell-cycle progression, routinely observed in G 1 prior to cell division commitment. Beyond this point, cells are presumed to complete S/G 2 /M at similar rates and in a size-independent manner. As such, studies of dependence between growth and division have focused on G 1 Moreover, in unicellular organisms, coordination between growth and division has commonly been analysed within the cycle of a single cell without accounting for correlations in growth and division characteristics between cycles of related cells. In a comprehensive analysis of three published time-lapse microscopy datasets, we analyse both intra- and inter-cycle dependencies between growth and division, revisiting assumptions about the coordination between these two processes. Interestingly, we find evidence (i) that S/G 2 /M durations are systematically longer in daughters than in mothers, (ii) of dependencies between S/G 2 /M and size at budding that echo the classical G 1 dependencies, and (iii) in contrast with recent bacterial studies, of negative dependencies between size at birth and size accumulated during the cell cycle. In addition, we develop a novel hierarchical model to uncover inter-cycle dependencies, and we find evidence for such dependencies in cells growing in sugar-poor environments. Our analysis highlights the need for experimentalists and modellers to account for new sources of cell-to-cell variation in growth and division, and our model provides a formal statistical framework for the continued study of dependencies between biological processes. © 2017 The Author(s).

Time-Lapse Cinemicrographic Studies of X-Irradiated HeLa S3 Cells

PubMed Central

Hurwitz, Camilla; Tolmach, L. J.

1969-01-01

Analysis of time-lapse cinemicrographs of X-irradiated HeLa S3 cells has shown that the incidence of cell fusion was increased from 0.9% (following 1267 divisions) in control cells to an average of 22% (following 655 divisions) in cells irradiated with 500 rad doses of 220 kv X-rays. The incidence depended on the stage of the generation cycle at which the parent cells were irradiated. It was nearly constant in the first three postirradiation generations. Fusion occurred at all stages of the generation cycle, but preferentially during the first 20%. Cells undergoing fusion progressed more slowly through the generation cycle and had a higher probability of disintegrating than did irradiated cells that did not fuse. The occurrence of fusion was clonally distributed in the population. It took place only between sister (or closely related) cells. Protoplasmic bridges were often visible between sister cells prior to fusion. Giant cells arose only as a result of fusion. The incidence of multipolar divisions, though higher than in unirradiated cells, was only 5.5% in cultures irradiated with 500 rads. Fusion occurred following 85% of the multipolar divisions and was often followed by a multipolar division. ImagesFigure 1 PMID:5807221

Worker Personality and Its Association with Spatially Structured Division of Labor

PubMed Central

Pamminger, Tobias; Foitzik, Susanne; Kaufmann, Katharina C.; Schützler, Natalie; Menzel, Florian

2014-01-01

Division of labor is a defining characteristic of social insects and fundamental to their ecological success. Many of the numerous tasks essential for the survival of the colony must be performed at a specific location. Consequently, spatial organization is an integral aspect of division of labor. The mechanisms organizing the spatial distribution of workers, separating inside and outside workers without central control, is an essential, but so far neglected aspect of division of labor. In this study, we investigate the behavioral mechanisms governing the spatial distribution of individual workers and its physiological underpinning in the ant Myrmica rubra. By investigating worker personalities we uncover position-associated behavioral syndromes. This context-independent and temporally stable set of correlated behaviors (positive association between movements and attraction towards light) could promote the basic separation between inside (brood tenders) and outside workers (foragers). These position-associated behavior syndromes are coupled with a high probability to perform tasks, located at the defined position, and a characteristic cuticular hydrocarbon profile. We discuss the potentially physiological causes for the observed behavioral syndromes and highlight how the study of animal personalities can provide new insights for the study of division of labor and self-organized processes in general. PMID:24497911

Effects of dimethyl sulfoxide on asymmetric division and cytokinesis in mouse oocytes.

PubMed

Zhou, Dongjie; Shen, Xinghui; Gu, Yanli; Zhang, Na; Li, Tong; Wu, Xi; Lei, Lei

2014-06-21

Dimethyl sulfoxide (DMSO) is used extensively as a permeable cryoprotectant and is a common solvent utilized for several water-insoluble substances. DMSO has various biological and pharmacological activities; however, the effect of DMSO on mouse oocyte meiotic maturation remains unknown. In DMSO-treated oocytes, we observed abnormal MII oocytes that contained large polar bodies, including 2-cell-like MII oocytes, during in vitro maturation. Oocyte polarization did not occur, due to the absence of actin cap formation and spindle migration. These features are among the primary causes of abnormal symmetric division; however, analysis of the mRNA expression levels of genes related to asymmetric division revealed no significant difference in the expression of these factors between the 3% DMSO-treated group and the control group. After each "blastomere" of the 2-cell-like MII stage oocytes was injected by one sperm head respectively, the oocytes still possessed the ability to extrude the second polar body from each "blastomere" and to begin cleavage. However, MII oocytes with large polar bodies developed to the blastocyst stage after intracytoplasmic sperm injection (ICSI). Furthermore, other permeable cryoprotectants, such as ethylene glycol and glycerol, also caused asymmetric division failure. Permeable cryoprotectants, such as DMSO, ethylene glycol, and glycerol, affect asymmetric division. DMSO disrupts cytokinesis completion by inhibiting cortical reorganization and polarization. Oocytes that undergo symmetric division maintain the ability to begin cleavage after ICSI.

Cyclin-dependent kinases: engines, clocks, and microprocessors.

PubMed

Morgan, D O

1997-01-01

Cyclin-dependent kinases (Cdks) play a well-established role in the regulation of the eukaryotic cell division cycle and have also been implicated in the control of gene transcription and other processes. Cdk activity is governed by a complex network of regulatory subunits and phosphorylation events whose precise effects on Cdk conformation have been revealed by recent crystallographic studies. In the cell, these regulatory mechanisms generate an interlinked series of Cdk oscillators that trigger the events of cell division.

COMPARISON OF FEMTOSECOND AND NANOSECOND TWO PHOTON ABSORPTION LASER INDUCED FLUORESCENCE (TALIF) OF ATOMIC OXYGEN IN ATMOSPHERIC PRESSURE PLASMAS

DTIC Science & Technology

2016-08-01

OXYGEN IN ATMOSPHERIC PRESSURE PLASMAS James D. Scofield (AFRL/RQQE) and James R. Gord (AFRL/RQTC) Electrical Systems Branch, Power and Control...Division (AFRL/RQQE) Combustion Branch, Turbine Engine Division (AFRL/RQTC) Jacob B. Schmidt and Sukesh Roy Spectral Energies LLC Brian Sands...LASER-INDUCED FLUORESCENCE (TALIF) OF ATOMIC OXYGEN IN ATMOSPHERIC PRESSURE PLASMAS 5a. CONTRACT NUMBER In-house 5b. GRANT NUMBER 5c. PROGRAM

Principles and Application of Magnetic Rubber Testing for Crack Detection in High-Strength Steel Components: I. Active-Field Inspection

DTIC Science & Technology

2014-12-01

Historically, MRT found its most extensive application in the inspection of critical high-strength steel components of the F-111 aircraft to...Steve Burke is Group Leader Acoustic Material Systems within Maritime Division and Task Leader for AIR 07/101 Assessment and Control of Aircraft ...Maritime Division. He has previously led research programs in advanced electromagnetic and ultrasonic NDE for aircraft applications. Geoff has BSc and BE

Strategic Defense Initiative Demonstration/Validation Program Environmental Assessment. Battle Management/Command and Control, and Communications (BM/C3),

DTIC Science & Technology

1987-08-01

POR A ENVIOMNE. STRATEGIC DEFENSE INITRIEDNU T IATI V EI 1 0193 O RTEI ONE..()SRTGC DEFENSE INITIATV E S RATION V EO ORORNIZATION WASINZGTON DC...facilities where Demonstration/Validation activities are planned.- e Ten areas of environmental consideration are addressed: (1) air quality; (2) . water...air quality, rater quality, and hazardous vaste (63). 2.2 ELCTRONIC SYSTEMS DIVISION The Electronic Systems Division administrative offices are located

Broadband Time Division Multiple Access (TDMA) Solution (Tech Insertion - C4 Enhancement for the U.S. Army in Transformation)

DTIC Science & Technology

2005-06-01

synchronization , timing and frequency to all of the traffic terminals through the reference burst. The MRT also periodically synchronizes all of the traffic...1 2005 Command and Control Research and Technology Symposium The Future of C2 Broadband Time Division Multiple Access (TDMA) Solution...UK Army) Dale White Barry Kruse Shawn White LTC Edward Eidson Thomas Mims COL Charles Dunn III Charlie Pangle Battle Command Battle

Quality Control Review of the Defense Intelligence Agency, Office of Inspector General, Audit Division

DTIC Science & Technology

2015-02-26

mission is to support the overall DIA OIG role of providing oversight of and reporting upon matters, which pertain to the performance of mission and...as discussed throughout this report. In the two IPERA audits, the DIA OIG Audit Division auditors found that DIA did not comply with the IPERA. For...Independence Not Followed For two of the three audits reviewed, the auditors did not follow the GAGAS conceptual framework for independence. GAGAS

More tooth, Less Skull: Force Structure Changes for an Uncertain Future

DTIC Science & Technology

2012-05-17

deployments in OIF and OEF are the archetypes for force employment and span of control, highlighting the limits of modularity and the creation of ad hoc...square miles in extent. There was a wider dispersion of formations to minimize the effect of an enemy’s tactical atomic weapons. At the same time, this...The sweeping reorganization to meet the requirements of the atomic battlefield became the Pentomic Division. The Pentomic Division, officially known

Cell division and density of symbiotic Chlorella variabilis of the ciliate Paramecium bursaria is controlled by the host's nutritional conditions during early infection process.

PubMed

Kodama, Yuuki; Fujishima, Masahiro

2012-10-01

The association of ciliate Paramecium bursaria with symbiotic Chlorella sp. is a mutualistic symbiosis. However, both the alga-free paramecia and symbiotic algae can still grow independently and can be reinfected experimentally by mixing them. Effects of the host's nutritional conditions against the symbiotic algal cell division and density were examined during early reinfection. Transmission electron microscopy revealed that algal cell division starts 24 h after mixing with alga-free P. bursaria, and that the algal mother cell wall is discarded from the perialgal vacuole membrane, which encloses symbiotic alga. Labelling of the mother cell wall with Calcofluor White Stain, a cell-wall-specific fluorochrome, was used to show whether alga had divided or not. Pulse labelling of alga-free P. bursaria cells with Calcofluor White Stain-stained algae with or without food bacteria for P. bursaria revealed that the fluorescence of Calcofluor White Stain in P. bursaria with bacteria disappeared within 3 days after mixing, significantly faster than without bacteria. Similar results were obtained both under constant light and dark conditions. This report is the first describing that the cell division and density of symbiotic algae of P. bursaria are controlled by the host's nutritional conditions during early infection. © 2012 Society for Applied Microbiology and Blackwell Publishing Ltd.

Electro-optic architecture (EOA) for sensors and actuators in aircraft propulsion systems

NASA Technical Reports Server (NTRS)

Glomb, W. L., Jr.

1989-01-01

Results of a study to design an optimal architecture for electro-optical sensing and control in advanced aircraft and space systems are described. The propulsion full authority digital Electronic Engine Control (EEC) was the focus for the study. The recommended architecture is an on-engine EEC which contains electro-optic interface circuits for fiber-optic sensors on the engine. Size and weight are reduced by multiplexing arrays of functionally similar sensors on a pair of optical fibers to common electro-optical interfaces. The architecture contains common, multiplex interfaces to seven sensor groups: (1) self luminous sensors; (2) high temperatures; (3) low temperatures; (4) speeds and flows; (5) vibration; (6) pressures; and (7) mechanical positions. Nine distinct fiber-optic sensor types were found to provide these sensing functions: (1) continuous wave (CW) intensity modulators; (2) time division multiplexing (TDM) digital optic codeplates; (3) time division multiplexing (TDM) analog self-referenced sensors; (4) wavelength division multiplexing (WDM) digital optic code plates; (5) wavelength division multiplexing (WDM) analog self-referenced intensity modulators; (6) analog optical spectral shifters; (7) self-luminous bodies; (8) coherent optical interferometers; and (9) remote electrical sensors. The report includes the results of a trade study including engine sensor requirements, environment, the basic sensor types, and relevant evaluation criteria. These figures of merit for the candidate interface types were calculated from the data supplied by leading manufacturers of fiber-optic sensors.

Earth Control and Investigations, Training Course 1972.

ERIC Educational Resources Information Center

Department of the Interior, Denver, CO. Engineering and Research Center.

Compiled in this notebook is material from an Earth Control and Investigations Course offered by the Earth Science Branch of the Division of General Research, Engineering and Research Center, Denver, Colorado. The training is designed to promote consistency and uniformity in control and investigation procedures throughout the Bureau of…

75 FR 55274 - Change of Address for Region 5 State and Local Agencies; Technical Correction

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-10

... Health District, Air Pollution Control, 33 Mill Street, Painesville, OH 44077. (xiv) Mahoning and...; Director, Canton City Health Department, Air Pollution Control Division, 420 Market Avenue North, Canton.... (xiii) Geauga and Lake Counties; Lake County General Health District, Air Pollution Control, 33 Mill...

Control of the proportion of inner cells by asymmetric divisions and the ensuing resilience of cloned rabbit embryos

PubMed Central

Duranthon, Véronique

2018-01-01

ABSTRACT Mammalian embryo cloning by nuclear transfer has a low success rate. This is hypothesized to correlate with a high variability of early developmental steps that segregate outer cells, which are fated to extra-embryonic tissues, from inner cells, which give rise to the embryo proper. Exploring the cell lineage of wild-type embryos and clones, imaged in toto until hatching, highlights the respective contributions of cell proliferation, death and asymmetric divisions to phenotypic variability. Preferential cell death of inner cells in clones, probably pertaining to the epigenetic plasticity of the transferred nucleus, is identified as a major difference with effects on the proportion of inner cell. In wild type and clones, similar patterns of outer cell asymmetric divisions are shown to be essential to the robust proportion of inner cells observed in wild type. Asymmetric inner cell division, which is not described in mice, is identified as a regulator of the proportion of inner cells and likely gives rise to resilient clones. PMID:29567671

Publications in biomedical and environmental sciences programs, 1981

DOE Office of Scientific and Technical Information (OSTI.GOV)

Moody, J.B.

1982-07-01

This bibliography contains 698 references to articles in journals, books, and reports published in the subject area of biomedical and environmental sciences during 1981. There are 520 references to articles published in journals and books and 178 references to reports. Staff members in the Biomedical and Environmental Sciences divisions have other publications not included in this bibliography; for example, theses, book reviews, abstracts published in journals or symposia proceedings, pending journal publications and reports such as monthly, bimonthly, and quarterly progress reports, contractor reports, and reports for internal distribution. This document is sorted by the division, and then alphabetically bymore » author. The sorting by divisions separates the references by subject area in a simple way. The divisions represented in the order that they appear in the bibliography are Analytical Chemistry, Biology, Chemical Technology, Information R and D, Health and Safety Research, Instrumentation and Controls, Computer Sciences, Energy, Engineering Technology, Solid State, Central Management, Operations, and Environmental Sciences. Indexes are provided by author, title, and journal reference.« less

The Yeast Cyclin-Dependent Kinase Routes Carbon Fluxes to Fuel Cell Cycle Progression.

PubMed

Ewald, Jennifer C; Kuehne, Andreas; Zamboni, Nicola; Skotheim, Jan M

2016-05-19

Cell division entails a sequence of processes whose specific demands for biosynthetic precursors and energy place dynamic requirements on metabolism. However, little is known about how metabolic fluxes are coordinated with the cell division cycle. Here, we examine budding yeast to show that more than half of all measured metabolites change significantly through the cell division cycle. Cell cycle-dependent changes in central carbon metabolism are controlled by the cyclin-dependent kinase (Cdk1), a major cell cycle regulator, and the metabolic regulator protein kinase A. At the G1/S transition, Cdk1 phosphorylates and activates the enzyme Nth1, which funnels the storage carbohydrate trehalose into central carbon metabolism. Trehalose utilization fuels anabolic processes required to reliably complete cell division. Thus, the cell cycle entrains carbon metabolism to fuel biosynthesis. Because the oscillation of Cdk activity is a conserved feature of the eukaryotic cell cycle, we anticipate its frequent use in dynamically regulating metabolism for efficient proliferation. Copyright © 2016 Elsevier Inc. All rights reserved.

Division and dynamic morphology of plastids.

PubMed

Osteryoung, Katherine W; Pyke, Kevin A

2014-01-01

Plastid division is fundamental to the biology of plant cells. Division by binary fission entails the coordinated assembly and constriction of four concentric rings, two internal and two external to the organelle. The internal FtsZ ring and external dynamin-like ARC5/DRP5B ring are connected across the two envelopes by the membrane proteins ARC6, PARC6, PDV1, and PDV2. Assembly-stimulated GTPase activity drives constriction of the FtsZ and ARC5/DRP5B rings, which together with the plastid-dividing rings pull and squeeze the envelope membranes until the two daughter plastids are formed, with the final separation requiring additional proteins. The positioning of the division machinery is controlled by the chloroplast Min system, which confines FtsZ-ring formation to the plastid midpoint. The dynamic morphology of plastids, especially nongreen plastids, is also considered here, particularly in relation to the production of stromules and plastid-derived vesicles and their possible roles in cellular communication and plastid functionality.

Analysis of Cell Division and Elongation Underlying the Developmental Acceleration of Root Growth in Arabidopsis thaliana1

PubMed Central

Beemster, Gerrit T.S.; Baskin, Tobias I.

1998-01-01

To investigate the relation between cell division and expansion in the regulation of organ growth rate, we used Arabidopsis thaliana primary roots grown vertically at 20°C with an elongation rate that increased steadily during the first 14 d after germination. We measured spatial profiles of longitudinal velocity and cell length and calculated parameters of cell expansion and division, including rates of local cell production (cells mm−1 h−1) and cell division (cells cell−1 h−1). Data were obtained for the root cortex and also for the two types of epidermal cell, trichoblasts and atrichoblasts. Accelerating root elongation was caused by an increasingly longer growth zone, while maximal strain rates remained unchanged. The enlargement of the growth zone and, hence, the accelerating root elongation rate, were accompanied by a nearly proportionally increased cell production. This increased production was caused by increasingly numerous dividing cells, whereas their rates of division remained approximately constant. Additionally, the spatial profile of cell division rate was essentially constant. The meristem was longer than generally assumed, extending well into the region where cells elongated rapidly. In the two epidermal cell types, meristem length and cell division rate were both very similar to that of cortical cells, and differences in cell length between the two epidermal cell types originated at the apex of the meristem. These results highlight the importance of controlling the number of dividing cells, both to generate tissues with different cell lengths and to regulate the rate of organ enlargement. PMID:9536070

Operation Eclipse; Appreciation and Outline Plan

DTIC Science & Technology

1945-04-25

the boundaries of GERMANY . " . • g« Engineering tools, equipment, plant and stores0 specially, adapted for military purposes. Bridging material of...Control Council ( Germany ) Control Commission for Germany ’ (British Element) Supreme Hq AEF Mission (Denmark) Supreme Headquarters, Allied...Mission (Norway) U.S. Group Control Council (Austria) Control Commission for Germany (Air Division) War Office, MO 1 (SP) • ’ Control Commission for

Auxin minimum triggers the developmental switch from cell division to cell differentiation in the Arabidopsis root

PubMed Central

De Ruvo, Micol; Pacifici, Elena; Salvi, Elena; Sozzani, Rosangela; Benfey, Philip N.; Di Paola, Luisa; Marée, Athanasius F. M.; Costantino, Paolo; Grieneisen, Verônica A.; Sabatini, Sabrina

2017-01-01

In multicellular organisms, a stringent control of the transition between cell division and differentiation is crucial for correct tissue and organ development. In the Arabidopsis root, the boundary between dividing and differentiating cells is positioned by the antagonistic interaction of the hormones auxin and cytokinin. Cytokinin affects polar auxin transport, but how this impacts the positional information required to establish this tissue boundary, is still unknown. By combining computational modeling with molecular genetics, we show that boundary formation is dependent on cytokinin’s control on auxin polar transport and degradation. The regulation of both processes shapes the auxin profile in a well-defined auxin minimum. This auxin minimum positions the boundary between dividing and differentiating cells, acting as a trigger for this developmental transition, thus controlling meristem size. PMID:28831001

Deregulation of cell growth and malignant transformation.

PubMed

Sulić, Sanda; Panić, Linda; Dikić, Ivan; Volarević, Sinisa

2005-08-01

Cell growth and cell division are fundamental aspects of cell behavior in all organisms. Recent insights from many model organisms have shed light on the molecular mechanisms that control cell growth and cell division. A significant body of evidence has now been accumulated, showing a direct link between deregulation of components of cell cycle machinery and cancer. In addition, defects in one or more steps that control growth are important for malignant transformation, as many tumor suppressors and proto-oncogenes have been found to regulate cell growth. The importance of cell growth in tumor development is further supported by the discovery that rapamycin, an effective anticancer drug, inhibits a key regulator of protein synthetic machinery and cell growth, mammalian target of rapamycin (mTOR). In most cases, cell growth and cell division are coupled, thereby maintaining cell size within physiological limits. We believe that, in a long-term perspective, understanding how these two processes are coordinated in vivo and how their interplay is deregulated in a number of diseases, including cancer, may have a direct impact on the efficiency of modern therapeutics.

Temporal Control of Plant Organ Growth by TCP Transcription Factors.

PubMed

Huang, Tengbo; Irish, Vivian F

2015-06-29

The Arabidopsis petal is a simple laminar organ whose development is largely impervious to environmental effects, making it an excellent model for dissecting the regulation of cell-cycle progression and post-mitotic cell expansion that together sculpt organ form. Arabidopsis petals grow via basipetal waves of cell division, followed by a phase of cell expansion. RABBIT EARS (RBE) encodes a C2H2 zinc finger transcriptional repressor and is required for petal development. During the early phase of petal initiation, RBE regulates a microRNA164-dependent pathway that controls cell proliferation at the petal primordium boundaries. The effects of rbe mutations on petal lamina growth suggest that RBE is also required to regulate later developmental events during petal organogenesis. Here, we demonstrate that, early in petal development, RBE represses the transcription of a suite of CIN-TCP genes that in turn act to inhibit the number and duration of cell divisions; the temporal alleviation of that repression results in the transition from cell division to post-mitotic cell expansion and concomitant petal maturation. Copyright © 2015 Elsevier Ltd. All rights reserved.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fujimura, Hiroaki

Mating pheromones, a- and {alpha}-factors, arrest the division of cells of opposite mating types, {alpha} and a cells, respectively. The author has isolated a sterile mutant of Saccharomyces cerevisiae using EMS that is defective in division arrest in response to {alpha}-factor but not defective in morphological changes and agglutinin induction. The mutation was designated dac2 for division arrest control by mating pheromones. The dac2 mutation was closely linked to gal1 and was different from the previously identified cell type nonspecific sterile mutations (ste4, ste5, ste7, ste11, ste12, ste18, and dac1). Although dac2 cells had no phenotype in the absence ofmore » pheromones, they showed morphological alterations and divided continuously in the presence of pheromones. As a result, dac2 cells had a mating defect. The dac2 mutation could suppress the lethality caused by the disruption of the GPA1 gene. These results suggest that the DAC2 product may control the signal for G-protein-mediated cell-cycle arrest and indicate that the synchronization of haploid yeast cell cycles by mating pheromones is essential for cell fusion during conjugation.« less

Negotiating jurisdiction in the workplace: a multiple-case study of nurse prescribing in hospital settings.

PubMed

Kroezen, M; Mistiaen, P; van Dijk, L; Groenewegen, P P; Francke, A L

2014-09-01

This paper reports on a multiple-case study of prescribing by nurse specialists in Dutch hospital settings. Most analyses of interprofessional negotiations over professional boundaries take a macro sociological approach and ignore workplace jurisdictions. Yet boundary blurring takes place and healthcare professionals renegotiate formal policies in the workplace. This paper studies the division of jurisdictional control over prescribing between nurse specialists and medical specialists in the workplace, and examines the relationship between workplace jurisdiction and legal jurisdiction over prescribing. Data collection took place in the Netherlands during the first half of 2013. The study used in-depth interviews with fifteen nurse specialists and fourteen medical specialists, non-participant observation of nurse specialists' prescribing consultations and document analysis. Great variety was found in the extent to which and way in which nurse specialists' legal prescriptive authority had been implemented. These findings suggest that there is considerable discrepancy between the division of jurisdictional control over prescribing at the macro (legal) level and the division at the micro (workplace) level. Copyright © 2014 Elsevier Ltd. All rights reserved.

The SsgA-like proteins in actinomycetes: small proteins up to a big task.

PubMed

Traag, Bjørn A; van Wezel, Gilles P

2008-06-01

Several unique protein families have been identified that play a role in the control of developmental cell division in streptomycetes. The SsgA-like proteins or SALPs, of which streptomycetes typically have at least five paralogues, control specific steps of sporulation-specific cell division in streptomycetes, affecting cell wall-related events such as septum localization and synthesis, thickening of the spore wall and autolytic spore separation. The expression level of SsgA, the best studied SALP, has a rather dramatic effect on septation and on hyphal morphology, which is not only of relevance for our understanding of (developmental) cell division but has also been successfully applied in industrial fermentation, to improve growth and production of filamentous actinomycetes. Recent observations suggest that SsgB most likely is the archetypal SALP, with only SsgB orthologues occurring in all morphologically complex actinomycetes. Here we review 10 years of research on the SsgA-like proteins in actinomycetes and discuss the most interesting regulatory, functional, phylogenetic and applied aspects of this relatively unknown protein family.

Inheritance of the Golgi Apparatus and Cytokinesis Are Controlled by Degradation of GBF1.

PubMed

Magliozzi, Roberto; Carrero, Zunamys I; Low, Teck Yew; Yuniati, Laurensia; Valdes-Quezada, Christian; Kruiswijk, Flore; van Wijk, Koen; Heck, Albert J R; Jackson, Catherine L; Guardavaccaro, Daniele

2018-06-12

Although much is known about how chromosome segregation is coupled to cell division, how intracellular organelles partition during mitotic division is poorly understood. We report that the phosphorylation-dependent degradation of the ARFGEF GBF1 regulates organelle trafficking during cell division. We show that, in mitosis, GBF1 is phosphorylated on Ser292 and Ser297 by casein kinase-2 allowing recognition by the F-box protein βTrCP. GBF1 interaction with βTrCP recruits GBF1 to the SCF βTrCP ubiquitin ligase complex, triggering its degradation. Phosphorylation and degradation of GBF1 occur along microtubules at the intercellular bridge of telophase cells and are required for Golgi membrane positioning and postmitotic Golgi reformation. Indeed, expression of a non-degradable GBF1 mutant inhibits the transport of the Golgi cluster adjacent to the midbody toward the Golgi twin positioned next to the centrosome and results in defective Golgi reassembly and cytokinesis failure. These findings define a mechanism that controls postmitotic Golgi reassembly and inheritance. Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.

PUBLICATIONS - AIR POLLUTION PREVENTION AND CONTROL TECHNOLOGY

EPA Science Inventory

The Air Pollution Prevention and Control Division (APPCD)publishes highly scientific and technical information developed through its four research branches. A list of key publications produced by the individual branches can be viewed by visiting the website for the respective bra...

The Identification and Care of Those at Highest Risk of Cancer

Cancer.gov

Frederick P. Li, MD, Chief, Cancer Control Program, Division of Cancer Epidemiology and Control, Adult Oncology Department at Dana-Farber/Harvard Cancer Center, Boston, MA, presented "The Identification and Care of Those at Highest Risk of Cancer".

Worklife and Wellness in Academic General Internal Medicine: Results from a National Survey.

PubMed

Linzer, Mark; Poplau, Sara; Babbott, Stewart; Collins, Tracie; Guzman-Corrales, Laura; Menk, Jeremiah; Murphy, Mary Lou; Ovington, Kay

2016-09-01

General internal medicine (GIM) careers are increasingly viewed as challenging and unsustainable. We aimed to assess academic GIM worklife and determine remediable predictors of stress and burnout. We conducted an email survey. Physicians, nurse practitioners, and physician assistants in 15 GIM divisions participated. A ten-item survey queried stress, burnout, and work conditions such as electronic medical record (EMR) challenges. An open-ended question assessed stressors and solutions. Results were categorized into burnout, high stress, high control, chaos, good teamwork, high values alignment, documentation time pressure, and excessive home EMR use. Frequencies were determined for national data, Veterans Affairs (VA) versus civilian populations, and hospitalist versus ambulatory roles. A General Linear Mixed Model (GLMM) evaluated associations with burnout. A formal content analysis was performed for open-ended question responses. Of 1235 clinicians sampled, 579 responded (47 %). High stress was present in 67 %, with 38 % burned out (burnout range 10-56 % by division). Half of respondents had low work control, 60 % reported high documentation time pressure, half described too much home EMR time, and most reported very busy or chaotic workplaces. Two-thirds felt aligned with departmental leaders' values, and three-quarters were satisfied with teamwork. Burnout was associated with high stress, low work control, and low values alignment with leaders (all p < 0.001). The 45 VA faculty had less burnout than civilian counterparts (17 % vs. 40 %, p < 0.05). Hospitalists described better teamwork than ambulatory clinicians and fewer hospitalists noted documentation time pressure (both p < 0.001). Key themes from the qualitative analysis were short visits, insufficient support staff, a Relative Value Unit mentality, documentation time pressure, and undervaluing education. While GIM divisions overall demonstrate high stress and burnout, division rates vary widely. Sustainability efforts within GIM could focus on visit length, staff support, schedule control, clinic chaos, and EMR stress.

Tidal impacts on the subtidal flow division at the main bifurcation in the Yangtze River Delta

NASA Astrophysics Data System (ADS)

Zhang, Wei; Feng, Haochuan; Hoitink, A. J. F.; Zhu, Yuliang; Gong, Fei; Zheng, Jinhai

2017-09-01

Flow division at bifurcations in the Yangtze Estuary has received ample attention, since it may control the pathways of terrestrial sediments over downstream river branches including the 12.5 m Deepwater Navigation channel. While some efforts have been made to interpret flow division at the bifurcations of the Yangtze Estuary, little attention has been paid to the role of tides. Flow division at estuarine bifurcations is made complicated by tides that propagate from the outlet of the tidal channels into the delta. To quantify the tidal influence on the distribution of river discharge, and more generally, to understand the mechanisms governing the subtidal flow division at the tidally affected bifurcation in the Yangtze River Delta, a two-dimensional hydrodynamic model is employed. In this model, the landward boundary is chosen beyond the tidal limit, where the tidal motion has faded out entirely. The seaward boundary is chosen such that the river discharge does not influence the water level. Subtidal discharges are decomposed using the method of factor separation, to distinguish between the effects of tides, river discharge and river-tide interactions on the subtidal flow division. Results indicate that tides modify the river discharge distribution over distributary channels in the Yangtze River Delta, particularly in the dry season. A significant difference in the subtidal flow division during spring tide and neap tide shows that the tidally averaged flow division over the distributaries in the delta greatly depends on tidal amplitude. By varying the river discharge at the landward boundary and amplitudes and phases of the principal tidal constituents at the seaward boundary of the established model, the sensitivities of the subtidal flow division to the river discharge and tidal amplitude variation were investigated in detail. Generally, the tidal impacts on the subtidal flow division are around 12% to 22%, with river discharge varying from 30,000 m3s-1 to 20,000 m3s-1. This effect on the flow distribution can even overwhelm the effects induced by river discharge based on geometry only, when the flow discharge is lowest. Furthermore, the fortnightly tidal cycle plays an important role in enhancing the inequality of the subtidal flow division caused by the M2 tidal component solely at the tidal bifurcation in the Yangtze River Delta during low flow.

Spontaneous Division and Motility in Active Nematic Droplets

NASA Astrophysics Data System (ADS)

Giomi, Luca; DeSimone, Antonio

2014-04-01

We investigate the mechanics of an active droplet endowed with internal nematic order and surrounded by an isotropic Newtonian fluid. Using numerical simulations we demonstrate that, due to the interplay between the active stresses and the defective geometry of the nematic director, this system exhibits two of the fundamental functions of living cells: spontaneous division and motility, by means of self-generated hydrodynamic flows. These behaviors can be selectively activated by controlling a single physical parameter, namely, an active variant of the capillary number.

DoD Veterinary Service Activity Role in DoD Food Safety.

DTIC Science & Technology

1998-01-01

medical research and development; zoonotic disease prevention and control; and food safety and quality assurance. The latter mission is not all encompassing...within DoD. This paper reviews the division of responsibilities, within DoD, for food safety and quality assurance. The complexity of the division...and the problem it causes joint operations planners are explored. A proposal for integrating overall strategic responsibility for food safety and quality assurance into the DoD Veterinary Service Activity is developed.

National Program for Inspection of Non-Federal Dams. Knox Mill Dam (ME 00276), Megunticook River Basin, Camden, Maine. Phase I Inspection Report.

DTIC Science & Technology

1978-08-01

been furnished the owner, Camden Water & Power Co., 33 Mechanic Street, ..... a ine 0,-43. Co-ies of this report will be made available to the public...gn Branch Engineering Division SAUL CO ER, Member Chief, Water Control Branch Engineering Division APPROVAL RECOMMENDED: JOE B. FRYAR Chief...Camden Water & Power Co. 33 Mechanic Street Camden, Maine 04843 Tne Camden Water and Power Company is an affiliate of Knox Woolen Mills Company. f

Free-space optics mode-wavelength division multiplexing system using LG modes based on decision feedback equalization

NASA Astrophysics Data System (ADS)

Amphawan, Angela; Ghazi, Alaan; Al-dawoodi, Aras

2017-11-01

A free-space optics mode-wavelength division multiplexing (MWDM) system using Laguerre-Gaussian (LG) modes is designed using decision feedback equalization for controlling mode coupling and combating inter symbol interference so as to increase channel diversity. In this paper, a data rate of 24 Gbps is achieved for a FSO MWDM channel of 2.6 km in length using feedback equalization. Simulation results show significant improvement in eye diagrams and bit-error rates before and after decision feedback equalization.

Networks Technology Conference

NASA Technical Reports Server (NTRS)

Tasaki, Keiji K. (Editor)

1993-01-01

The papers included in these proceedings represent the most interesting and current topics being pursued by personnel at GSFC's Networks Division and supporting contractors involved in Space, Ground, and Deep Space Network (DSN) technical work. Although 29 papers are represented in the proceedings, only 12 were presented at the conference because of space and time limitations. The proceedings are organized according to five principal technical areas of interest to the Networks Division: Project Management; Network Operations; Network Control, Scheduling, and Monitoring; Modeling and Simulation; and Telecommunications Engineering.

Divide and shape: an endosymbiont in action.

PubMed

Pyke, Kevin A

2013-02-01

The endosymbiotic evolution of the plastid within the host cell required development of a mechanism for efficient division of the plastid. Whilst a model for the mechanism of chloroplast division has been constructed, little is known of how other types of plastids divide, especially the proplastid, the progenitor of all plastid types in the cell. It has become clear that plastid shape is highly heterogeneous and dynamic, especially stromules. This article considers how such variation in morphology might be controlled and how such plastids might divide efficiently.

Modification of cytogenetic and physiological effects of space flight factors by biologically active compounds

NASA Technical Reports Server (NTRS)

Aliyev, A. A.; Mekhti-Zade, E. R.; Mashinskiy, A. L.; Alekperov, U. K.

1986-01-01

Physiological and cytogenetic changes in the Welsh onion plants induced by a short (82 days) and long term (522 days) space flight are expressed in decrease of seed germination, inhibition of stem growth, depression of cell division in root meristem, and increase in the number of structural chromosome rearrangements. The treatment of such plants with solutions of a-tocopherol, auxin, and kinetin decreased the level of chromosome aberrations to the control one and normalized cell divisions and growth partly or completely.

Title V Evaluation Reports - Nevada

EPA Pesticide Factsheets

Title V Operating Permit Program Evaluation Final Reports: Nevada Department of Conservation & Natural Resources, Division of Environmental Protection, Bureau of Air Pollution Control and Clark County

40 CFR 147.2250 - State-administered program-Class I, III, IV, and V wells.

Code of Federal Regulations, 2011 CFR

2011-07-01

... of the Federal Register on June 25, 1984. (1) Utah Water Pollution Control Act, Utah Code Annotated... Executive Secretary of Utah Water Pollution Control Committee on August 16, 1990). (b) Other laws. The... Department of Health, Division of Environmental Health, Bureau of Water Pollution Control, to EPA Region VIII...

40 CFR 147.2250 - State-administered program-Class I, III, IV, and V wells.

Code of Federal Regulations, 2010 CFR

2010-07-01

... of the Federal Register on June 25, 1984. (1) Utah Water Pollution Control Act, Utah Code Annotated... Executive Secretary of Utah Water Pollution Control Committee on August 16, 1990). (b) Other laws. The... Department of Health, Division of Environmental Health, Bureau of Water Pollution Control, to EPA Region VIII...

Trade Electricity. Motors & Controls--Level 3. Standardized Curriculum.

ERIC Educational Resources Information Center

New York City Board of Education, Brooklyn, NY. Office of Occupational and Career Education.

This curriculum guide consists of seven modules on motors and controls, one of the three divisions of the standardized trade electricity curriculum in high schools in New York City. The seven modules cover the following subjects: energy conservation wiring, direct current (DC) motor repair and rewinding, DC motor controls, alternating current (AC)…

IFLA General Conference, 1986. Bibliographic Control Division. Section: Bibliography. Papers.

ERIC Educational Resources Information Center

International Federation of Library Associations and Institutions, The Hague (Netherlands).

Papers on bibliographic control presented at the 1986 International Federation of Library Associations (IFLA) conference include: (1) "Bibliographic Interchange/Coordination in Southeast Asia (Huck Tee Lim, Malaysia); (2) "Project for 'Chinese National Bibliography' and Its Progress" (Huang Jungui, China); (3) "Subject…

76 FR 29756 - Healthcare Infection Control Practices Advisory Committee (HICPAC)

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-23

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention Healthcare... Director, Division of Healthcare Quality Promotion regarding (1) The practice of healthcare infection... infections), antimicrobial resistance, and related events in settings where healthcare is provided; and (3...

76 FR 63622 - Healthcare Infection Control Practices Advisory Committee, (HICPAC)

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-13

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Disease Control and Prevention Healthcare... Director, Division of Healthcare Quality Promotion regarding (1) The practice of healthcare infection... infections), antimicrobial resistance, and related events in settings where healthcare is provided; and (3...

Some karyological observations on plants grown in space

NASA Technical Reports Server (NTRS)

Krikorian, A. D.; Oconnor, S. A.

1982-01-01

Experiments were conducted to assess whether cell division in a plant root would be affected by prolonged exposure to microgravity. Root materials from sunflower, oat, and mung bean plants grown on STS-2 and STS-3 were utilized for the experiments. It is found that all oat, sunflower, and mung seedlings showed a reduced number of cells in division as they went through their first cell division cycle on earth when compared to their ground controls. A significant number of oat, mung, and sunflower plantlets exhibited random root orientation and the lack of strictly orthotropic growth of their shoot systems in the flight samples. In addition, it is found that the mung roots were apparently least affected in terms of their cytology despite the fact that their roots were often randomly oriented.

A satellite mobile communication system based on Band-Limited Quasi-Synchronous Code Division Multiple Access (BLQS-CDMA)

NASA Technical Reports Server (NTRS)

Degaudenzi, R.; Elia, C.; Viola, R.

1990-01-01

Discussed here is a new approach to code division multiple access applied to a mobile system for voice (and data) services based on Band Limited Quasi Synchronous Code Division Multiple Access (BLQS-CDMA). The system requires users to be chip synchronized to reduce the contribution of self-interference and to make use of voice activation in order to increase the satellite power efficiency. In order to achieve spectral efficiency, Nyquist chip pulse shaping is used with no detection performance impairment. The synchronization problems are solved in the forward link by distributing a master code, whereas carrier forced activation and closed loop control techniques have been adopted in the return link. System performance sensitivity to nonlinear amplification and timing/frequency synchronization errors are analyzed.

Ethylene is an endogenous stimulator of cell division in the cambial meristem of Populus

PubMed Central

Love, Jonathan; Björklund, Simon; Vahala, Jorma; Hertzberg, Magnus; Kangasjärvi, Jaakko; Sundberg, Björn

2009-01-01

The plant hormone ethylene is an important signal in plant growth responses to environmental cues. In vegetative growth, ethylene is generally considered as a regulator of cell expansion, but a role in the control of meristem growth has also been suggested based on pharmacological experiments and ethylene-overproducing mutants. In this study, we used transgenic ethylene-insensitive and ethylene-overproducing hybrid aspen (Populus tremula × tremuloides) in combination with experiments using an ethylene perception inhibitor [1-methylcyclopropene (1-MCP)] to demonstrate that endogenous ethylene produced in response to leaning stimulates cell division in the cambial meristem. This ethylene-controlled growth gives rise to the eccentricity of Populus stems that is formed in association with tension wood. PMID:19293381

Design of CMOS compatible and compact, thermally-compensated electro-optic modulator based on off-axis microring resonator for dense wavelength division multiplexing applications.

PubMed

Haldar, Raktim; Banik, Abhik D; Varshney, Shailendra K

2014-09-22

In this work, we propose and demonstrate the performance of silicon-on-insulator (SOI) off-axis microring resonator (MRR) as electro-optic modulator (EOM). Adding an extra off-axis inner-ring in conventional microring structure provides control to compensate thermal effects on EOM. It is shown that dynamically controlled bias-voltage applied to the outer ring has the potency to quell the thermal effects over a wide range of temperature. Thus, besides the appositely biased conventional microring, off-axis inner microring with pre-emphasized electrical input message signal enables our proposed structure suitable for high data-rate dense wavelength division multiplexing scheme of optical communication within a very compact device size.

Endosymbiosis in trypanosomatid protozoa: the bacterium division is controlled during the host cell cycle

PubMed Central

Catta-Preta, Carolina M. C.; Brum, Felipe L.; da Silva, Camila C.; Zuma, Aline A.; Elias, Maria C.; de Souza, Wanderley; Schenkman, Sergio; Motta, Maria Cristina M.

2015-01-01

Mutualism is defined as a beneficial relationship for the associated partners and usually assumes that the symbiont number is controlled. Some trypanosomatid protozoa co-evolve with a bacterial symbiont that divides in coordination with the host in a way that results in its equal distribution between daughter cells. The mechanism that controls this synchrony is largely unknown, and its comprehension might provide clues to understand how eukaryotic cells evolved when acquiring symbionts that later became organelles. Here, we approached this question by studying the effects of inhibitors that affect the host exclusively in two symbiont-bearing trypanosomatids, Strigomonas culicis and Angomonas deanei. We found that inhibiting host protein synthesis using cycloheximide or host DNA replication using aphidicolin did not affect the duplication of bacterial DNA. Although the bacteria had autonomy to duplicate their DNA when host protein synthesis was blocked by cycloheximide, they could not complete cytokinesis. Aphidicolin promoted the inhibition of the trypanosomatid cell cycle in the G1/S phase, leading to symbiont filamentation in S. culicis but not in A. deanei. Treatment with camptothecin blocked the host protozoa cell cycle in the G2 phase and induced the formation of filamentous symbionts in both species. Oryzalin, which affects host microtubule polymerization, blocked trypanosomatid mitosis and abrogated symbiont division. Our results indicate that host factors produced during the cell division cycle are essential for symbiont segregation and may control the bacterial cell number. PMID:26082757

Effects of dimethyl sulfoxide on asymmetric division and cytokinesis in mouse oocytes

PubMed Central

2014-01-01

Background Dimethyl sulfoxide (DMSO) is used extensively as a permeable cryoprotectant and is a common solvent utilized for several water-insoluble substances. DMSO has various biological and pharmacological activities; however, the effect of DMSO on mouse oocyte meiotic maturation remains unknown. Results In DMSO-treated oocytes, we observed abnormal MII oocytes that contained large polar bodies, including 2-cell–like MII oocytes, during in vitro maturation. Oocyte polarization did not occur, due to the absence of actin cap formation and spindle migration. These features are among the primary causes of abnormal symmetric division; however, analysis of the mRNA expression levels of genes related to asymmetric division revealed no significant difference in the expression of these factors between the 3% DMSO-treated group and the control group. After each “blastomere” of the 2-cell–like MII stage oocytes was injected by one sperm head respectively, the oocytes still possessed the ability to extrude the second polar body from each “blastomere” and to begin cleavage. However, MII oocytes with large polar bodies developed to the blastocyst stage after intracytoplasmic sperm injection (ICSI). Furthermore, other permeable cryoprotectants, such as ethylene glycol and glycerol, also caused asymmetric division failure. Conclusion Permeable cryoprotectants, such as DMSO, ethylene glycol, and glycerol, affect asymmetric division. DMSO disrupts cytokinesis completion by inhibiting cortical reorganization and polarization. Oocytes that undergo symmetric division maintain the ability to begin cleavage after ICSI. PMID:24953160

FORESEE: Fully Outsourced secuRe gEnome Study basEd on homomorphic Encryption

PubMed Central

2015-01-01

Background The increasing availability of genome data motivates massive research studies in personalized treatment and precision medicine. Public cloud services provide a flexible way to mitigate the storage and computation burden in conducting genome-wide association studies (GWAS). However, data privacy has been widely concerned when sharing the sensitive information in a cloud environment. Methods We presented a novel framework (FORESEE: Fully Outsourced secuRe gEnome Study basEd on homomorphic Encryption) to fully outsource GWAS (i.e., chi-square statistic computation) using homomorphic encryption. The proposed framework enables secure divisions over encrypted data. We introduced two division protocols (i.e., secure errorless division and secure approximation division) with a trade-off between complexity and accuracy in computing chi-square statistics. Results The proposed framework was evaluated for the task of chi-square statistic computation with two case-control datasets from the 2015 iDASH genome privacy protection challenge. Experimental results show that the performance of FORESEE can be significantly improved through algorithmic optimization and parallel computation. Remarkably, the secure approximation division provides significant performance gain, but without missing any significance SNPs in the chi-square association test using the aforementioned datasets. Conclusions Unlike many existing HME based studies, in which final results need to be computed by the data owner due to the lack of the secure division operation, the proposed FORESEE framework support complete outsourcing to the cloud and output the final encrypted chi-square statistics. PMID:26733391

Control of cell division and the spatial localization of assembled gene products in Caulobacter crescentus

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nathan, P.D.

Experiments are described that examine the role of penicillin-binding proteins (PBPs) in the regulation of cell division in Caulobacter crescentus; and the spatial localization of methyl-accepting chemotaxis proteins (MCPs) in C. crescentus swarmer and predivisional cells. In the analysis of PBP function, in vivo and in vitro assays are used to directly label C. crescentus PBPs with (/sup 3/H) penicillin G in wild type strain CB15, in a series of conditional cell division mutants and in new temperature sensitive cephalosporin C resistant mutants PC8002 and PC8003. 14 PBPs are characterized and a high molecular weight PBP (PBP 1B) that ismore » required for cell division is identified. PBP 1B competes for ..beta..-lactams that induce filament formation and may be a high affinity binding protein. A second high molecular weight PBP (PBP 1C) is also associated with defective cell division. The examination of PBP patterns in synchronous swarmer cells reveals that the in vivo activity of PBP 1B and PBP 1C increases at the time that the cell division pathway is initiated. None of the PBPs, however, appear to be differentially localized in the C. crescentus cell. In the analysis of MCP localization, in vivo and in vitro assays are used to directly label C. crescentus MCPs with methyl-/sup 3/H. MCPs are examined in flagellated and non-flagellated vesicles prepared from cells by immunoaffinity chromatography.« less

FORESEE: Fully Outsourced secuRe gEnome Study basEd on homomorphic Encryption.

PubMed

Zhang, Yuchen; Dai, Wenrui; Jiang, Xiaoqian; Xiong, Hongkai; Wang, Shuang

2015-01-01

The increasing availability of genome data motivates massive research studies in personalized treatment and precision medicine. Public cloud services provide a flexible way to mitigate the storage and computation burden in conducting genome-wide association studies (GWAS). However, data privacy has been widely concerned when sharing the sensitive information in a cloud environment. We presented a novel framework (FORESEE: Fully Outsourced secuRe gEnome Study basEd on homomorphic Encryption) to fully outsource GWAS (i.e., chi-square statistic computation) using homomorphic encryption. The proposed framework enables secure divisions over encrypted data. We introduced two division protocols (i.e., secure errorless division and secure approximation division) with a trade-off between complexity and accuracy in computing chi-square statistics. The proposed framework was evaluated for the task of chi-square statistic computation with two case-control datasets from the 2015 iDASH genome privacy protection challenge. Experimental results show that the performance of FORESEE can be significantly improved through algorithmic optimization and parallel computation. Remarkably, the secure approximation division provides significant performance gain, but without missing any significance SNPs in the chi-square association test using the aforementioned datasets. Unlike many existing HME based studies, in which final results need to be computed by the data owner due to the lack of the secure division operation, the proposed FORESEE framework support complete outsourcing to the cloud and output the final encrypted chi-square statistics.

The TCP4 transcription factor of Arabidopsis blocks cell division in yeast at G1 {yields} S transition

DOE Office of Scientific and Technical Information (OSTI.GOV)

Aggarwal, Pooja; Padmanabhan, Bhavna; Bhat, Abhay

2011-07-01

Highlights: {yields} TCP4 is a class II TCP transcription factor, that represses cell division in Arabidopsis. {yields} TCP4 expression in yeast retards cell division by blocking G1 {yields} S transition. {yields} Genome-wide expression studies and Western analysis reveals stabilization of cell cycle inhibitor Sic1, as possible mechanism. -- Abstract: The TCP transcription factors control important aspects of plant development. Members of class I TCP proteins promote cell cycle by regulating genes directly involved in cell proliferation. In contrast, members of class II TCP proteins repress cell division. While it has been postulated that class II proteins induce differentiation signal, theirmore » exact role on cell cycle has not been studied. Here, we report that TCP4, a class II TCP protein from Arabidopsis that repress cell proliferation in developing leaves, inhibits cell division by blocking G1 {yields} S transition in budding yeast. Cells expressing TCP4 protein with increased transcriptional activity fail to progress beyond G1 phase. By analyzing global transcriptional status of these cells, we show that expression of a number of cell cycle genes is altered. The possible mechanism of G1 {yields} S arrest is discussed.« less

Home Advantage in Men's and Women's Spanish First and Second Division Water Polo Leagues.

PubMed

Prieto, Jaime; Gómez, Miguel-Ángel; Pollard, Richard

2013-01-01

The purpose of this study was to quantify the home advantage in both men's and women's First and Second Division water polo leagues, to compare the results obtained according to sex of participants and the level of competition, and to test for possible differences in home advantage when considering the interaction between these two factors. The sample comprised four seasons from 2007-2008 to 2010-2011 for a total of 1942 games analyzed. The results showed the existence of home advantage in both men's and women's First and Second Divisions. After controlling for the competitive balance of each league in each season, there was a significant difference between men's and women's leagues, with higher home advantage for men's leagues (58.60% compared with 53.70% for women's leagues). There was also a significant difference between the levels of competition, with greater home advantage for the Second Division (57.95% compared with 54.35% for First Division). No significant differences in home advantage were found when considering the interaction between sex of participants and the level of competition. The results in relation to sex of participants and the level of competition are consistent with previous studies in other sports such as football or handball.

Home Advantage in Men’s and Women’s Spanish First and Second Division Water Polo Leagues

PubMed Central

Prieto, Jaime; Gómez, Miguel-Ángel; Pollard, Richard

The purpose of this study was to quantify the home advantage in both men’s and women’s First and Second Division water polo leagues, to compare the results obtained according to sex of participants and the level of competition, and to test for possible differences in home advantage when considering the interaction between these two factors. The sample comprised four seasons from 2007–2008 to 2010–2011 for a total of 1942 games analyzed. The results showed the existence of home advantage in both men’s and women’s First and Second Divisions. After controlling for the competitive balance of each league in each season, there was a significant difference between men’s and women’s leagues, with higher home advantage for men’s leagues (58.60% compared with 53.70% for women’s leagues). There was also a significant difference between the levels of competition, with greater home advantage for the Second Division (57.95% compared with 54.35% for First Division). No significant differences in home advantage were found when considering the interaction between sex of participants and the level of competition. The results in relation to sex of participants and the level of competition are consistent with previous studies in other sports such as football or handball. PMID:24146714

Visual speech perception in foveal and extrafoveal vision: further implications for divisions in hemispheric projections.

PubMed

Jordan, Timothy R; Sheen, Mercedes; Abedipour, Lily; Paterson, Kevin B

2014-01-01

When observing a talking face, it has often been argued that visual speech to the left and right of fixation may produce differences in performance due to divided projections to the two cerebral hemispheres. However, while it seems likely that such a division in hemispheric projections exists for areas away from fixation, the nature and existence of a functional division in visual speech perception at the foveal midline remains to be determined. We investigated this issue by presenting visual speech in matched hemiface displays to the left and right of a central fixation point, either exactly abutting the foveal midline or else located away from the midline in extrafoveal vision. The location of displays relative to the foveal midline was controlled precisely using an automated, gaze-contingent eye-tracking procedure. Visual speech perception showed a clear right hemifield advantage when presented in extrafoveal locations but no hemifield advantage (left or right) when presented abutting the foveal midline. Thus, while visual speech observed in extrafoveal vision appears to benefit from unilateral projections to left-hemisphere processes, no evidence was obtained to indicate that a functional division exists when visual speech is observed around the point of fixation. Implications of these findings for understanding visual speech perception and the nature of functional divisions in hemispheric projection are discussed.

Activities of the Structures Division, Lewis Research Center

NASA Technical Reports Server (NTRS)

1990-01-01

The purpose of the NASA Lewis Research Center, Structures Division's 1990 Annual Report is to give a brief, but comprehensive, review of the technical accomplishments of the Division during the past calendar year. The report is organized topically to match the Center's Strategic Plan. Over the years, the Structures Division has developed the technology base necessary for improving the future of aeronautical and space propulsion systems. In the future, propulsion systems will need to be lighter, to operate at higher temperatures and to be more reliable in order to achieve higher performance. Achieving these goals is complex and challenging. Our approach has been to work cooperatively with both industry and universities to develop the technology necessary for state-of-the-art advancement in aeronautical and space propulsion systems. The Structures Division consists of four branches: Structural Mechanics, Fatigue and Fracture, Structural Dynamics, and Structural Integrity. This publication describes the work of the four branches by three topic areas of Research: (1) Basic Discipline; (2) Aeropropulsion; and (3) Space Propulsion. Each topic area is further divided into the following: (1) Materials; (2) Structural Mechanics; (3) Life Prediction; (4) Instruments, Controls, and Testing Techniques; and (5) Mechanisms. The publication covers 78 separate topics with a bibliography containing 159 citations. We hope you will find the publication interesting as well as useful.

16 CFR 801.1 - Definitions.

Code of Federal Regulations, 2010 CFR

2010-01-01

... means an ultimate parent entity and all entities which it controls directly or indirectly. Examples: 1... parent corporations, subsidiaries and divisions (whether consolidated or unconsolidated, and whether... controls them is not an “entity” (see § 801.1(a)(2)). Corporations A and B* are the ultimate parent...

16 CFR 801.1 - Definitions.

Code of Federal Regulations, 2011 CFR

2011-01-01

... means an ultimate parent entity and all entities which it controls directly or indirectly. Examples: 1... parent corporations, subsidiaries and divisions (whether consolidated or unconsolidated, and whether... controls them is not an “entity” (see § 801.1(a)(2)). Corporations A and B* are the ultimate parent...

16 CFR 801.1 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-01-01

... means an ultimate parent entity and all entities which it controls directly or indirectly. Examples: 1... parent corporations, subsidiaries and divisions (whether consolidated or unconsolidated, and whether... controls them is not an “entity” (see § 801.1(a)(2)). Corporations A and B* are the ultimate parent...

16 CFR 801.1 - Definitions.

Code of Federal Regulations, 2012 CFR

2012-01-01

... means an ultimate parent entity and all entities which it controls directly or indirectly. Examples: 1... parent corporations, subsidiaries and divisions (whether consolidated or unconsolidated, and whether... controls them is not an “entity” (see § 801.1(a)(2)). Corporations A and B* are the ultimate parent...

16 CFR 801.1 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-01-01

... means an ultimate parent entity and all entities which it controls directly or indirectly. Examples: 1... parent corporations, subsidiaries and divisions (whether consolidated or unconsolidated, and whether... controls them is not an “entity” (see § 801.1(a)(2)). Corporations A and B* are the ultimate parent...

40 CFR 766.7 - Submission of information.

Code of Federal Regulations, 2013 CFR

2013-07-01

....20) and must be addressed to: Document Control Office, (7407), Information Management Division... 40 Protection of Environment 32 2013-07-01 2013-07-01 false Submission of information. 766.7... CONTROL ACT DIBENZO-PARA-DIOXINS/DIBENZOFURANS General Provisions § 766.7 Submission of information. All...

40 CFR 766.7 - Submission of information.

Code of Federal Regulations, 2012 CFR

2012-07-01

....20) and must be addressed to: Document Control Office, (7407), Information Management Division... 40 Protection of Environment 32 2012-07-01 2012-07-01 false Submission of information. 766.7... CONTROL ACT DIBENZO-PARA-DIOXINS/DIBENZOFURANS General Provisions § 766.7 Submission of information. All...

40 CFR 766.7 - Submission of information.

Code of Federal Regulations, 2011 CFR

2011-07-01

....20) and must be addressed to: Document Control Office, (7407), Information Management Division... 40 Protection of Environment 31 2011-07-01 2011-07-01 false Submission of information. 766.7... CONTROL ACT DIBENZO-PARA-DIOXINS/DIBENZOFURANS General Provisions § 766.7 Submission of information. All...

40 CFR 766.7 - Submission of information.

Code of Federal Regulations, 2010 CFR

2010-07-01

....20) and must be addressed to: Document Control Office, (7407), Information Management Division... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Submission of information. 766.7... CONTROL ACT DIBENZO-PARA-DIOXINS/DIBENZOFURANS General Provisions § 766.7 Submission of information. All...

IFLA General Conference, 1987. Division of Bibliographic Control. Cataloging Section. Papers.

ERIC Educational Resources Information Center

International Federation of Library Associations, The Hague (Netherlands).

The five papers in this collection focus on bibliographic control: (1) "Developments and Progress in Bibliography" (B. C. Bloomfield, Chairman, Section on Bibliography); (2) "Developments and Progress in Cataloguing" (Tom Delsey, Chairman, Section on Cataloging); (3) "Developments and Progress in Classification and…

Combination of Synthetic Chemistry and Live-Cell Imaging Identified a Rapid Cell Division Inhibitor in Tobacco and Arabidopsis thaliana.

PubMed

Nambo, Masakazu; Kurihara, Daisuke; Yamada, Tomomi; Nishiwaki-Ohkawa, Taeko; Kadofusa, Naoya; Kimata, Yusuke; Kuwata, Keiko; Umeda, Masaaki; Ueda, Minako

2016-11-01

Cell proliferation is crucial to the growth of multicellular organisms, and thus the proper control of cell division is important to prevent developmental arrest or overgrowth. Nevertheless, tools for controlling cell proliferation are still poor in plant. To develop novel tools, we focused on a specific compound family, triarylmethanes, whose members show various antiproliferative activities in animals. By combining organic chemistry to create novel and diverse compounds containing the triarylmethyl moiety and biological screens based on live-cell imaging of a fluorescently labeled tobacco Bright Yellow-2 (BY-2) culture cell line (Nicotiana tabacum), we isolated (3-furyl)diphenylmethane as a strong but partially reversible inhibitor of plant cell division. We also found that this agent had efficient antiproliferative activity in developing organs of Arabidopsis thaliana without causing secondary defects in cell morphology, and induced rapid cell division arrest independent of the cell cycle stage. Given that (3-furyl)diphenylmethane did not affect the growth of a human cell line (HeLa) and a budding yeast (Saccharomyces cerevisiae), it should act specifically on plants. Taking our results together, we propose that the combination of desired chemical synthesis and detailed biological analysis is an effective tool to create novel drugs, and that (3-furyl)diphenylmethane is a specific antiproliferative agent for plants. © The Author 2016. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.

Administrative Services Division - Alaska Department of Law

Science.gov Websites

Attorney General Opinions Executive Branch Ethics Criminal Justice Alaska Medicaid Fraud Control Anchorage digital communication. Department-wide Information Technology issues, strategic planning, resource

40 CFR 86.1801-12 - Applicability.

Code of Federal Regulations, 2011 CFR

2011-07-01

... brand of the producing manufacturer but were sold by or otherwise under the control of another... the following address: Director, Compliance and Innovative Strategies Division, U.S. Environmental...

Guidance, Navigation and Control Innovations at the NASA Goddard Space Flight Center

NASA Technical Reports Server (NTRS)

Ericsson, Aprille Joy

2002-01-01

A viewgraph presentation on guidance navigation and control innovations at the NASA Goddard Space Flight Center is presented. The topics include: 1) NASA's vision; 2) NASA's Mission; 3) Earth Science Enterprise (ESE); 4) Guidance, Navigation and Control Division (GN&C); 5) Landsat-7 Earth Observer-1 Co-observing Program; and 6) NASA ESE Vision.

Molecular Programs Underlying Asymmetric Stem Cell Division and Their Disruption in Malignancy.

PubMed

Mukherjee, Subhas; Brat, Daniel J

2017-01-01

Asymmetric division of stem cells is a highly conserved and tightly regulated process by which a single stem cell produces two unequal daughter cells. One retains its stem cell identity while the other becomes specialized through a differentiation program and loses stem cell properties. Coordinating these events requires control over numerous intra- and extracellular biological processes and signaling networks. In the initial stages, critical events include the compartmentalization of fate determining proteins within the mother cell and their subsequent passage to the appropriate daughter cell in order to direct their destiny. Disturbance of these events results in an altered dynamic of self-renewing and differentiation within the cell population, which is highly relevant to the growth and progression of cancer. Other critical events include proper asymmetric spindle assembly, extrinsic regulation through micro-environmental cues, and non-canonical signaling networks that impact cell division and fate determination. In this review, we discuss mechanisms that maintain the delicate balance of asymmetric cell division in normal tissues and describe the current understanding how some of these mechanisms are deregulated in cancer.

Inhibition of cell division in hupA hupB mutant bacteria lacking HU protein.

PubMed Central

Dri, A M; Rouviere-Yaniv, J; Moreau, P L

1991-01-01

Escherichia coli hupA hypB double mutants that lack HU protein have severe cellular defects in cell division, DNA folding, and DNA partitioning. Here we show that the sfiA11 mutation, which alters the SfiA cell division inhibitor, reduces filamentation and production of anucleate cells in AB1157 hupA hupB strains. However, lexA3(Ind-) and sfiB(ftsZ)114 mutations, which normally counteract the effect of the SfiA inhibitor, could not restore a normal morphology to hupA hupB mutant bacteria. The LexA repressor, which controls the expression of the sfiA gene, was present in hupA hupB mutant bacteria in concentrations half of those of the parent bacteria, but this decrease was independent of the specific cleavage of the LexA repressor by activated RecA protein. One possibility to account for the filamentous morphology of hupA hupB mutant bacteria is that the lack of HU protein alters the expression of specific genes, such as lexA and fts cell division genes. Images PMID:2019558

POINTS-OF-CONTACT (AIR POLLUTION TECHNOLOGY BRANCH, AIR POLLUTION PREVENTION AND CONTROL DIVISION, NRMRL)

EPA Science Inventory

The Air Pollution Technology Branch's (APTB) Point-of-Contact page lists APTB research areas along with the name, telephone number, and e-mail address for each responsible person. APTB's research areas include NOx Control, Hazardous Waste Incineration, Municipal Waste Combustion,...

76 FR 78732 - Petition for Waiver of Compliance

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-19

... or modification of a signal system. FRA has assigned the petition Docket Number FRA-2011-0092. CSX seeks approval of the proposed modification of the bridge tender controlled signals to automatic signals..., Jacksonville Division. The modification consists of the conversion of bridge tender controlled signals to...

77 FR 47394 - Proposed Data Collections Submitted for Public Comment and Recommendations

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-08

...--New-- National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP), Centers for... and Health Promotion, Division of Cancer Prevention and Control (DCPC). Through NCCCP, CDC supports... environmental changes to guide sustainable cancer control; (5) promote health equity as it relates to cancer...

2015 New Grantee Workshop Overview | DCCPS/NCI/NIH

Cancer.gov

At the 2015 New Grantee Workshop, the Division of Cancer Control & Population Sciences (DCCPS) brought together approximately forty new investigators who received their first R01 in 2012 and 2013 to build a strong and vibrant cancer control research program and to help advance their careers.

76 FR 29251 - Guidance for Industry and Food and Drug Administration Staff; Class II Special Controls; Guidance...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-20

...: Topical Oxygen Chamber for Extremities; Availability; Correction AGENCY: Food and Drug Administration, HHS... Special Controls Guidance Documents: Topical Oxygen Chamber for Extremities.'' The document published... Oxygen Chamber for Extremities'' to the Division of Small Manufacturers, International, and Consumer...

A Microbial Avenue to Cell Cycle Control in the Plant Superkingdom[C][W][OPEN

PubMed Central

Tulin, Frej; Cross, Frederick R.

2014-01-01

Research in yeast and animals has resulted in a well-supported consensus model for eukaryotic cell cycle control. The fit of this model to early diverging eukaryotes, such as the plant kingdom, remains unclear. Using the green alga Chlamydomonas reinhardtii, we developed an efficient pipeline, incorporating robotics, semiautomated image analysis, and deep sequencing, to molecularly identify >50 genes, mostly conserved in higher plants, specifically required for cell division but not cell growth. Mutated genes include the cyclin-dependent kinases CDKA (resembling yeast and animal Cdk1) and the plant-specific CDKB. The Chlamydomonas cell cycle consists of a long G1 during which cells can grow >10-fold, followed by multiple rapid cycles of DNA replication and segregation. CDKA and CDKB execute nonoverlapping functions: CDKA promotes transition between G1 and entry into the division cycle, while CDKB is essential specifically for spindle formation and nuclear division, but not for DNA replication, once CDKA-dependent initiation has occurred. The anaphase-promoting complex is required for similar steps in the Chlamydomonas cell cycle as in Opisthokonts; however, the spindle assembly checkpoint, which targets the APC in Opisthokonts, appears severely attenuated in Chlamydomonas, based on analysis of mutants affecting microtubule function. This approach allows unbiased integration of the consensus cell cycle control model with innovations specific to the plant lineage. PMID:25336509

Energy-Efficient Next-Generation Passive Optical Networks Based on Sleep Mode and Heuristic Optimization

NASA Astrophysics Data System (ADS)

Zulai, Luis G. T.; Durand, Fábio R.; Abrão, Taufik

2015-05-01

In this article, an energy-efficiency mechanism for next-generation passive optical networks is investigated through heuristic particle swarm optimization. Ten-gigabit Ethernet-wavelength division multiplexing optical code division multiplexing-passive optical network next-generation passive optical networks are based on the use of a legacy 10-gigabit Ethernet-passive optical network with the advantage of using only an en/decoder pair of optical code division multiplexing technology, thus eliminating the en/decoder at each optical network unit. The proposed joint mechanism is based on the sleep-mode power-saving scheme for a 10-gigabit Ethernet-passive optical network, combined with a power control procedure aiming to adjust the transmitted power of the active optical network units while maximizing the overall energy-efficiency network. The particle swarm optimization based power control algorithm establishes the optimal transmitted power in each optical network unit according to the network pre-defined quality of service requirements. The objective is controlling the power consumption of the optical network unit according to the traffic demand by adjusting its transmitter power in an attempt to maximize the number of transmitted bits with minimum energy consumption, achieving maximal system energy efficiency. Numerical results have revealed that it is possible to save 75% of energy consumption with the proposed particle swarm optimization based sleep-mode energy-efficiency mechanism compared to 55% energy savings when just a sleeping-mode-based mechanism is deployed.

Psychological Sciences Division 1979 Programs.

DTIC Science & Technology

1979-11-01

the potential for substantially improving the ONR effectiveness of Navy undersea manipulator sys- tems. Computer aided controls can be used to Report...OPERATOR VIEWING AND CONTROL OF the operator enters control orders) to determine UNDERSEA VEHICLE AND WORK SYS- ihe structure and mode of command inputs...efforts focus upon a class of control-display ele- Current Navy submersible work systems, such as ments common to general purpose undersea work CURV

Research Programs & Initiatives

Cancer.gov

CGH develops international initiatives and collaborates with other NCI divisions, NCI-designated Cancer Centers, and other countries to support cancer control planning, encourage capacity building, and support cancer research and research networks.

Informed Consent

MedlinePlus

... Education Achieving Zero Preventable Deaths Conference Publications and Posters National Trauma System Injury Prevention and Control Quality ... Abstracts Hotel Accommodations Travel Information Moderator/Speaker ... Poster Presenter Information 2017 Presentations Education Education Overview Division ...

42 CFR 7.4 - Schedule of charges.

Code of Federal Regulations, 2013 CFR

2013-10-01

... is available from the Division of Scientific Resources, Centers for Disease Control, 1600 Clifton..., and a proportionate share of management and administrative costs), and the cost of particular...

42 CFR 7.4 - Schedule of charges.

Code of Federal Regulations, 2014 CFR

2014-10-01

... is available from the Division of Scientific Resources, Centers for Disease Control, 1600 Clifton..., and a proportionate share of management and administrative costs), and the cost of particular...

Frank Princiotta

EPA Pesticide Factsheets

Frank Princiotta retired from EPA in 2015 after a 43-year career with the agency as Director of the Air Pollution Prevention and Control Division (APPCD) of the National Risk Management Research Laboratory.

National Program for Inspection of Non-Federal Dams. Keyser Dam (VT 00097), Connecticut River Basin, Chelsea, Vermont. Phase I Inspection Report.

DTIC Science & Technology

1980-04-01

CARNEY M. TERZIAN, HENBER I Design Branch Engineering Division RICHARD DIB * Water Control Branch Engineering Division [ hPIPWVAL 220ininu: Chief...2 f. Operator 2 I g. Purpose of Dam 2 h. Design and Construction History 2 i. Normal Operational Procedure 2 1.3 Pertinent Data 2 a. Drainage...i. Spillway 5 J. Regulating Outlets 5 [I h] Section Page 2. ENGINEERING DATA 6 2.1 Design Data 6 2.2 Construction Data 6 2.3 Operation Data 6 2.4

National Program for Inspection of Non-Federal Dams. Williams Pond Dam (CT 00551), Thames River Basin, Lebanon, Connecticut. Phase I Inspection Program.

DTIC Science & Technology

1978-10-01

GRA&I UnTucea B WILLIAMS POND DAM ~~1Z~ CT 00551 _ Distribution/ Availabilit Y Codes Avail and/or Dis~tj pecialS RIVER BASIN ~lIILEBANON, COXNNECTICUT...Inspection Report. Alternatives to these recommendations r 1 would include reducing the Williams Pond water levels during expected periods of intense storm...Materials Branch Engi’neering Division FRED J. VNS. Jr., Member Chief, De ’ggn Branch Engineering Division SAUL COOPER, -r Chief, Water Control Branch

Comparison of FDMA and CDMA for second generation land-mobile satellite communications

NASA Technical Reports Server (NTRS)

Yongacoglu, A.; Lyons, R. G.; Mazur, B. A.

1990-01-01

Code Division Multiple Access (CDMA) and Frequency Division Multiple Access (FDMA) (both analog and digital) systems capacities are compared on the basis of identical link availabilities and physical propagation models. Parameters are optimized for a bandwidth limited, multibeam environment. For CDMA, the benefits of voice activated carriers, antenna discrimination, polarization reuse, return link power control and multipath suppression are included in the analysis. For FDMA, the advantages of bandwidth efficient modulation/coding combinations, voice activated carriers, polarization reuse, beam placement, and frequency staggering were taken into account.

Multi-carrier mobile TDMA system with active array antenna

NASA Technical Reports Server (NTRS)

Suzuki, Ryutaro; Matsumoto, Yasushi; Hamamoto, Naokazu

1990-01-01

A multi-carrier time division multiple access (TDMA) is proposed for the future mobile satellite communications systems that include a multi-satellite system. This TDMA system employs the active array antenna in which the digital beam forming technique is adopted to control the antenna beam direction. The antenna beam forming is carried out at the base band frequency by using the digital signal processing technique. The time division duplex technique is applied for the TDM/TDMA burst format, in order not to overlap transmit and receive timing.

Excavation of attractor modules for nasopharyngeal carcinoma via integrating systemic module inference with attract method.

PubMed

Jiang, T; Jiang, C-Y; Shu, J-H; Xu, Y-J

2017-07-10

The molecular mechanism of nasopharyngeal carcinoma (NPC) is poorly understood and effective therapeutic approaches are needed. This research aimed to excavate the attractor modules involved in the progression of NPC and provide further understanding of the underlying mechanism of NPC. Based on the gene expression data of NPC, two specific protein-protein interaction networks for NPC and control conditions were re-weighted using Pearson correlation coefficient. Then, a systematic tracking of candidate modules was conducted on the re-weighted networks via cliques algorithm, and a total of 19 and 38 modules were separately identified from NPC and control networks, respectively. Among them, 8 pairs of modules with similar gene composition were selected, and 2 attractor modules were identified via the attract method. Functional analysis indicated that these two attractor modules participate in one common bioprocess of cell division. Based on the strategy of integrating systemic module inference with the attract method, we successfully identified 2 attractor modules. These attractor modules might play important roles in the molecular pathogenesis of NPC via affecting the bioprocess of cell division in a conjunct way. Further research is needed to explore the correlations between cell division and NPC.

Equilibrium between cell division and apoptosis in immortal cells as an alternative to the G1 restriction mechanism in mammalian cells.

PubMed

Dedov, Vadim N; Dedova, Irina V; Nicholson, Garth A

2004-04-01

Starvation arrests cultured mammalian cells in the G(1) restriction point of the cell cycle, whereas cancer cells generally lose the regulatory control of the cell cycle. Human lymphocytes, infected with Epstein-Barr virus (EBV), also lose their cell cycle control and produce immortal lymphoblastoid cell lines. We show that during starvation, EBV-lymphoblasts override the cell cycle arrest in the G(1) restriction point and continue cell division. Simultaneously, starvation activates apoptosis in an approximately half of the daughter cells in each cell generation. Continuos cell division and partial removal of cells by apoptosis results in stabilization of viable cell numbers, where a majority of viable cells are in the G(1) phase of the cell cycle. In contrast to starvation, anticancer drug etoposide activates apoptosis indiscriminately in all EBV-lymphoblasts and convertes all the viable cells into apoptotic. We conclude that the removal of surplus cells by apoptosis may represent a survival mechanism of transformed (i.e., cancer) cell population in nutrient restricted conditions, whereas nontransformed mammalian cells are arrested in the G(1) restriction point of the cell cycle.

The SsgA-like proteins in actinomycetes: small proteins up to a big task

PubMed Central

Traag, Bjørn A.

2008-01-01

Several unique protein families have been identified that play a role in the control of developmental cell division in streptomycetes. The SsgA-like proteins or SALPs, of which streptomycetes typically have at least five paralogues, control specific steps of sporulation-specific cell division in streptomycetes, affecting cell wall-related events such as septum localization and synthesis, thickening of the spore wall and autolytic spore separation. The expression level of SsgA, the best studied SALP, has a rather dramatic effect on septation and on hyphal morphology, which is not only of relevance for our understanding of (developmental) cell division but has also been succesfully applied in industrial fermentation, to improve growth and production of filamentous actinomycetes. Recent observations suggest that SsgB most likely is the archetypal SALP, with only SsgB orthologues occurring in all morphologically complex actinomycetes. Here we review 10 years of research on the SsgA-like proteins in actinomycetes and discuss the most interesting regulatory, functional, phylogenetic and applied aspects of this relatively unknown protein family. Electronic supplementary material The online version of this article (doi:10.1007/s10482-008-9225-3) contains supplementary material, which is available to authorized users. PMID:18273689

5 CFR 2601.301 - Accounting of gifts.

Code of Federal Regulations, 2010 CFR

2010-01-01

... accounted for by following appropriate internal controls and accounting procedures. (b) The Administration Division shall maintain an inventory of donated personal property valued at over $500. The inventory shall...

Epidemiology & Genomics Research Program

Cancer.gov

The Epidemiology and Genomics Research Program, in the National Cancer Institute's Division of Cancer Control and Population Sciences, funds research in human populations to understand the determinants of cancer occurrence and outcomes.

23 CFR 645.207 - Definitions.

Code of Federal Regulations, 2010 CFR

2010-04-01

... inspection and copying from the FHWA Washington Headquarters and all FHWA Division Offices as prescribed in... also mean the utility company inclusive of any substantially owned or controlled subsidiary. For the...

The effect of desferrioxamine on transferrin receptors, the cell cycle and growth rates of human leukaemic cells.

PubMed Central

Bomford, A; Isaac, J; Roberts, S; Edwards, A; Young, S; Williams, R

1986-01-01

The effect of the iron chelator, desferrioxamine, on transferrin binding, growth rates and the cell cycle was investigated in the human leukaemic cell line, K562. At all concentrations of the chelator (2-50 microM) binding of 125I-transferrin was increased by 24 h and reached a maximum at 72-96 h. Maximum binding (6-8-fold increased) occurred in cells treated with 20 microM-desferrioxamine, in contrast with control cells which, at 96 h, showed a 50% decrease over initial binding. Scatchard analysis at 4 degrees C showed that this increased binding was due to an increase in the number of receptors, as the Kd was similar in induced (1.8 nM) and control (1.5 nM) cells. After 96 h cells, cultured with 20 and 50 microM-desferrioxamine accumulated 59Fe from bovine transferrin at over twice the rate found with control cells, reflecting the increase in transferrin receptors. Although iron uptake was unimpaired by the chelator there was a dose-dependent inhibition of cell growth, with control cells completing three divisions in 96 h and those in 10 microM-desferrioxamine only two divisions. At the highest concentration (50 microM), cell division was abrogated although cell viability was maintained (85%). In contrast, DNA synthesis was not markedly affected, except at 50 microM-desferrioxamine when incorporation of [3H]thymidine was 52% of that in control cells. Flow cytometry revealed that there was a progressive accumulation of the cells in the active phases of their cycle (S, G2 + M). Desferrioxamine may increase transferrin receptors in two ways: by chelating a regulatory pool of iron within the cell, and by arresting cells in S phase when receptors are maximally expressed. PMID:3790074

A Two-Year Longitudinal MRI Study of the Corpus Callosum in Autism

PubMed Central

Frazier, Thomas W.; Keshavan, Matcheri S.; Minshew, Nancy J.; Hardan, Antonio Y.

2015-01-01

A growing body of literature has identified size reductions of the corpus callosum (CC) in autism. However, to our knowledge, no published studies have reported on the growth of CC volumes in youth with autism. Volumes of the total CC and its sub-divisions were obtained from 23 male children with autism and 23 age-matched male controls at baseline and 2-year follow-up. Persistent reductions in total CC volume were observed in participants with autism relative to controls. Only the rostral body sub-division showed a normalization of size over time. Persistent reductions are consistent with the diagnostic stability and life-long impairment observed in many individuals with autism. Multimodal imaging studies are needed to identify specific fiber tracks contributing to CC reductions. PMID:22350341

76 FR 59162 - Notice Pursuant to the National Cooperative Research and Production Act of 1993-National Center...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-23

... Lake, IL; Climax Portable Machine Tools, Inc., Newberg, OR; Clockwork Solutions, Inc. (CSI), Austin, TX..., MA; University of Texas Austin, Austin, TX; Vista Controls, Inc., dba Curtiss-Wright Controls... DEPARTMENT OF JUSTICE Antitrust Division Notice Pursuant to the National Cooperative Research and...

QUALITY CONTROL FOR RESEARCH STUDIES: A CRITICAL PART OF THE QUALITY SYSTEM AT THE U. S. EPA

EPA Science Inventory

QUALITY CONTROL FOR RESEARCH STUDIES: A CRITICAL PART OF THE QUALITY SYSTEM AT THE U.S. EPA Mette C.J. Schladweiler, Scientist, and Thomas J. Hughes, QA and Records Manager, Experimental Toxicology Division (ETD), National Health and Environmental Effects Research Laboratory (NHE...

40 CFR 1060.105 - What diurnal requirements apply for equipment?

Code of Federal Regulations, 2014 CFR

2014-07-01

...) AIR POLLUTION CONTROLS CONTROL OF EVAPORATIVE EMISSIONS FROM NEW AND IN-USE NONROAD AND STATIONARY... meet the diurnal emission standards adopted by the California Air Resources Board in the Final Regulation Order, Article 1, Chapter 15, Division 3, Title 13, California Code of Regulations, July 26, 2004...

40 CFR 1060.105 - What diurnal requirements apply for equipment?

Code of Federal Regulations, 2013 CFR

2013-07-01

...) AIR POLLUTION CONTROLS CONTROL OF EVAPORATIVE EMISSIONS FROM NEW AND IN-USE NONROAD AND STATIONARY... meet the diurnal emission standards adopted by the California Air Resources Board in the Final Regulation Order, Article 1, Chapter 15, Division 3, Title 13, California Code of Regulations, July 26, 2004...

40 CFR 1060.105 - What diurnal requirements apply for equipment?

Code of Federal Regulations, 2010 CFR

2010-07-01

...) AIR POLLUTION CONTROLS CONTROL OF EVAPORATIVE EMISSIONS FROM NEW AND IN-USE NONROAD AND STATIONARY... equipment may optionally meet the diurnal emission standards adopted by the California Air Resources Board in the Final Regulation Order, Article 1, Chapter 15, Division 3, Title 13, California Code of...

40 CFR 1060.105 - What diurnal requirements apply for equipment?

Code of Federal Regulations, 2011 CFR

2011-07-01

...) AIR POLLUTION CONTROLS CONTROL OF EVAPORATIVE EMISSIONS FROM NEW AND IN-USE NONROAD AND STATIONARY... meet the diurnal emission standards adopted by the California Air Resources Board in the Final Regulation Order, Article 1, Chapter 15, Division 3, Title 13, California Code of Regulations, July 26, 2004...

40 CFR 1060.105 - What diurnal requirements apply for equipment?

Code of Federal Regulations, 2012 CFR

2012-07-01

...) AIR POLLUTION CONTROLS CONTROL OF EVAPORATIVE EMISSIONS FROM NEW AND IN-USE NONROAD AND STATIONARY... meet the diurnal emission standards adopted by the California Air Resources Board in the Final Regulation Order, Article 1, Chapter 15, Division 3, Title 13, California Code of Regulations, July 26, 2004...

IFLA General Conference, 1984. Bibliographic Control Division. Sections on Bibliography, Cataloging, and Classification. Part 1. Papers.

ERIC Educational Resources Information Center

International Federation of Library Associations, The Hague (Netherlands).

Papers on bibliography, cataloging, and classification presented at the 1984 IFLA general conference include: (1) "Bibliographic Control: Zimbabwe's Present Practices and Hopes for the Future" (Angeline S. Kamba, Zimbabwe); (2) "Classification and Subject Cataloguing in Kenya" (Margaret N. Muriuki, Kenya); (3)…

RESEARCH AREA -- MUNICIPAL WASTE COMBUSTION (AIR POLLUTION TECHNOLOGY BRANCH, AIR POLLUTION PREVENTION AND CONTROL DIVISION, NRMRL)

EPA Science Inventory

The municipal waste combustion (MWC) program supports the development of revised rules for air pollutant emissions from the MWC source category. Basic research is performed on MWC pollutant formation and control mechanisms for acid gas, trace organic, and trace metal emissions. T...

Publications - GMC 380 | Alaska Division of Geological & Geophysical

Science.gov Websites

Publications Geologic Materials Center General Information Inventory Monthly Report Hours and Location Policy DGGS GMC 380 Publication Details Title: 1974 control survey report for Orange Hill, Alaska Authors ): Nabesna Bibliographic Reference Smith, W.H., 2010, 1974 control survey report for Orange Hill, Alaska

75 FR 53370 - RailAmerica, Inc., Palm Beach Holding, Inc., RailAmerica Transportation Corp., Central Railroad...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-31

...America, with Fortress retaining indirect control of RailAmerica, in Fortress Inv. Group, LLC et al..., Chicago Ft. Wayne and Eastern Railroad Division, Fortress Investment Group, LLC, and RR Acquisition Holding, LLC--Control Exemption--Delphos Terminal Company, Inc. AGENCY: Surface Transportation Board...

VISITOR - SULTAN - JSC

NASA Image and Video Library

1985-04-04

S85-29711 (April 1985) --- Ronald C. Epps, right of the training division in the mission operations directorate, briefs the Saudi Arabian payload specialist, Sultan Salman Abdelazize Al-Saud, and his backup, Abdulmohsen Hamad Al-Bassam, in the flight control room (FCR) of the mission control center (MCC). Erlinda Stevenson is also pictured.

Cataloguing and Classification Section. Bibliographic Control Division. Papers.

ERIC Educational Resources Information Center

International Federation of Library Associations, The Hague (Netherlands).

Papers on cataloging, classification, and coding systems which were presented at the 1982 International Federation of Library Associations (IFLA) conference include: (1) "Numbering and Coding Systems for Bibliographic Control in Use in North America" by Lois Mai Chan (United States); (2) "A Project Undertaken by the Library of…

DOE Office of Scientific and Technical Information (OSTI.GOV)

Roy, Madhuparna, E-mail: mroy17@jhmi.edu; Itoh, Kie, E-mail: kito5@jhmi.edu; Iijima, Miho, E-mail: miijima@jhmi.edu

The cycle of mitochondrial division and fusion disconnect and reconnect individual mitochondria in cells to remodel this energy-producing organelle. Although dynamin-related protein 1 (Drp1) plays a major role in mitochondrial division in cells, a reduced level of mitochondrial division still persists even in the absence of Drp1. It is unknown how much Drp1-mediated mitochondrial division accounts for the connectivity of mitochondria. The role of a Parkinson’s disease-associated protein—parkin, which biochemically and genetically interacts with Drp1—in mitochondrial connectivity also remains poorly understood. Here, we quantified the number and connectivity of mitochondria using mitochondria-targeted photoactivatable GFP in cells. We show that themore » loss of Drp1 increases the connectivity of mitochondria by 15-fold in mouse embryonic fibroblasts (MEFs). While a single loss of parkin does not affect the connectivity of mitochondria, the connectivity of mitochondria significantly decreased compared with a single loss of Drp1 when parkin was lost in the absence of Drp1. Furthermore, the loss of parkin decreased the frequency of depolarization of the mitochondrial inner membrane that is caused by increased mitochondrial connectivity in Drp1-knockout MEFs. Therefore, our data suggest that parkin negatively regulates Drp1-indendent mitochondrial division. -- Highlights: •A Drp1-mediated mechanism accounts for ∼95% of mitochondrial division. •Parkin controls the connectivity of mitochondria via a mechanism that is independent of Drp1. •In the absence of Drp1, connected mitochondria transiently depolarize. •The transient depolarization is independent of calcium signaling and uncoupling protein 2.« less

Physalis floridana Cell Number Regulator1 encodes a cell membrane-anchored modulator of cell cycle and negatively controls fruit size

PubMed Central

Li, Zhichao; He, Chaoying

2015-01-01

Physalis species show a significant variation in berry size; however, the underlying molecular basis is unknown. In this work, we showed that cell division difference in the ovaries might contribute to the ultimate berry size variation within Physalis species, and that mRNA abundance of Physalis floridana Cell Number Regulator1 (PfCNR1), the putative orthologue of the tomato fruit weight 2.2 (FW2.2), was negatively correlated with cell division in the ovaries. Moreover, heterochronic expression variation of the PfCNR1 genes in the ovaries concomitantly correlated with berry weight variation within Physalis species. In transgenic Physalis, multiple organ sizes could be negatively controlled by altering PfCNR1 levels, and cell division instead of cell expansion was primarily affected. PfCNR1 was shown to be anchored in the plasma membrane and to interact with PfAG2 (an AGAMOUS-like protein determining ovary identity). The expression of PfCYCD2;1, a putative orthologue of the mitosis-specific gene CyclinD2;1 in the cell cycle was negatively correlated with the PfCNR1 mRNA levels. PfAG2 was found to selectively bind to the CArG-box in the PfCYCD2;1 promoter and to repress PfCYCD2;1 expression, thus suggesting a PfAG2-mediated pathway for PfCNR1 to regulate cell division. The interaction of PfCNR1 with PfAG2 enhanced the repression of PfCYCD2;1 expression. The nuclear import of PfAG2 was essential in the proposed pathway. Our data provide new insights into the developmental pathways of a cell membrane-anchored protein that modulates cell division and governs organ size determination. This study also sheds light on the link between organ identity and organ growth in plants. PMID:25305759

14 CFR 204.7 - Revocation for dormancy.

Code of Federal Regulations, 2010 CFR

2010-01-01

.... Such filings shall be addressed to the Documentary Services Division, Department of Transportation... carrier by the FAA. (Approved by the Office of Management and Budget under control number 2106-0023) ...

Metacognition in Upper-Division Biology Students: Awareness Does Not Always Lead to Control

ERIC Educational Resources Information Center

Dye, Kathryn Morris; Stanton, Julie Dangremond

2017-01-01

Students with awareness and control of their own thinking can learn more and perform better than students who are not metacognitive. Metacognitive regulation is how you control your thinking in order to learn. It includes the skill of evaluation, which is the ability to appraise your approaches to learning and then modify future plans based on…

Acupressure in Controlling Nausea in Young Patients Receiving Highly Emetogenic Chemotherapy | Division of Cancer Prevention

Cancer.gov

RATIONALE: Acupressure wristbands may prevent or reduce nausea and caused by chemotherapy. It is not yet known whether standard care is more effective with or without acupressure wristbands in controlling acute and delayed nausea. PURPOSE: This randomized phase III trial is studying how well acupressure wristbands work with or without standard care in controlling nausea in

Mitochondrial division/mitophagy inhibitor (Mdivi) Ameliorates Pressure Overload Induced Heart Failure

PubMed Central

Givvimani, Srikanth; Munjal, Charu; Tyagi, Neetu; Sen, Utpal; Metreveli, Naira; Tyagi, Suresh C.

2012-01-01

Background We have previously reported the role of anti-angiogenic factors in inducing the transition from compensatory cardiac hypertrophy to heart failure and the significance of MMP-9 and TIMP-3 in promoting this process during pressure overload hemodynamic stress. Several studies reported the evidence of cardiac autophagy, involving removal of cellular organelles like mitochondria (mitophagy), peroxisomes etc., in the pathogenesis of heart failure. However, little is known regarding the therapeutic role of mitochondrial division inhibitor (Mdivi) in the pressure overload induced heart failure. We hypothesize that treatment with mitochondrial division inhibitor (Mdivi) inhibits abnormal mitophagy in a pressure overload heart and thus ameliorates heart failure condition. Materials and Methods To verify this, ascending aortic banding was done in wild type mice to create pressure overload induced heart failure and then treated with Mdivi and compared with vehicle treated controls. Results Expression of MMP-2, vascular endothelial growth factor, CD31, was increased, while expression of anti angiogenic factors like endostatin and angiostatin along with MMP-9, TIMP-3 was reduced in Mdivi treated AB 8 weeks mice compared to vehicle treated controls. Expression of mitophagy markers like LC3 and p62 was decreased in Mdivi treated mice compared to controls. Cardiac functional status assessed by echocardiography showed improvement and there is also a decrease in the deposition of fibrosis in Mdivi treated mice compared to controls. Conclusion Above results suggest that Mdivi inhibits the abnormal cardiac mitophagy response during sustained pressure overload stress and propose the novel therapeutic role of Mdivi in ameliorating heart failure. PMID:22479323

Application of AI techniques to blast furnace operations

DOE Office of Scientific and Technical Information (OSTI.GOV)

Iida, Osamu; Ushijima, Yuichi; Sawada, Toshiro

1995-10-01

It was during the first stages of application of artificial intelligence (AI) to industrial fields, that the ironmaking division of Mizushima works at Kawasaki Steel recognized its potential. Since that time, the division has sought applications for these techniques to solve various problems. AI techniques applied to control the No. 3 blast furnace operations at the Mizushima works include: Blast furnace control by a diagnostic type of expert system that gives guidance to the actions required for blast furnace operation as well as control of furnace heat by automatically setting blast temperature; Hot stove combustion control by a combination ofmore » fuzzy inference and a physical model to insure good thermal efficiency of the stove; and blast furnace burden control using neural networks makes it possible to connect the pattern of gas flow distribution with the condition of the furnace. Experience of AI to control the blast furnace and other ironmaking operations has proved its capability for achieving automation and increased operating efficiency. The benefits are very high. For these reasons, the applications of AI techniques will be extended in the future and new techniques studied to further improve the power of AI.« less

Architecture and inherent robustness of a bacterial cell-cycle control system.

PubMed

Shen, Xiling; Collier, Justine; Dill, David; Shapiro, Lucy; Horowitz, Mark; McAdams, Harley H

2008-08-12

A closed-loop control system drives progression of the coupled stalked and swarmer cell cycles of the bacterium Caulobacter crescentus in a near-mechanical step-like fashion. The cell-cycle control has a cyclical genetic circuit composed of four regulatory proteins with tight coupling to processive chromosome replication and cell division subsystems. We report a hybrid simulation of the coupled cell-cycle control system, including asymmetric cell division and responses to external starvation signals, that replicates mRNA and protein concentration patterns and is consistent with observed mutant phenotypes. An asynchronous sequential digital circuit model equivalent to the validated simulation model was created. Formal model-checking analysis of the digital circuit showed that the cell-cycle control is robust to intrinsic stochastic variations in reaction rates and nutrient supply, and that it reliably stops and restarts to accommodate nutrient starvation. Model checking also showed that mechanisms involving methylation-state changes in regulatory promoter regions during DNA replication increase the robustness of the cell-cycle control. The hybrid cell-cycle simulation implementation is inherently extensible and provides a promising approach for development of whole-cell behavioral models that can replicate the observed functionality of the cell and its responses to changing environmental conditions.

Growth patterns of the Cambrian microbialite: Phototropism and speciation of Epiphyton

NASA Astrophysics Data System (ADS)

Woo, J.; Chough, S. K.

2010-07-01

Microbes started constructing shallow marine stromatolitic bioherms in the Archean, but they transferred their role as a major buildup maker to metazoans in Phanerozoic. Microbial buildups often recovered their predominance in the carbonate platform when reefal metazoan communities collapsed. Epiphyton, an extinct taxon of calcified microbe that possessed branching filamentous trichomes, was an important reef builder in the shallow marine carbonate platform during Middle Cambrian, aftermath of an extinction of archaeocyath sponges which were major reef-building sessile organisms in the Early Cambrian. Here we present direct evidence of phototropism of Epiphyton, found from fossilized behavior in micro- and macro-structures of meter-scale microbial bioherms of the Zhangxia Formation (Middle Cambrian), North China Platform, Shandong Province, China. The bioherms consist of stacked growth layers with the inner and outer divisions divided by distinct boundary. The inner division of growth layers of the Epiphyton bioherm is dominated by dense uniform bush-shaped Epiphyton thalli, whereas the curved outer division has layered texture normal to the surface, comprised of elongated and chambered thalli. It suggests that photosynthetic Epiphyton reacted actively to the spatial changes in intensity of sunlight, controlled by angle of illumination on the curved growth surface of the bioherm. The inner and the outer divisions comprise different morpho-types of Epiphyton. The spatial distributions of different morpho-types in variously illuminated divisions of Epiphyton might have caused further speciation of Epiphyton.

TDM1 Regulation Determines the Number of Meiotic Divisions

PubMed Central

Cifuentes, Marta; Jolivet, Sylvie; Cromer, Laurence; Harashima, Hirofumi; Bulankova, Petra; Renne, Charlotte; Crismani, Wayne; Nomura, Yuko; Nakagami, Hirofumi; Sugimoto, Keiko; Schnittger, Arp; Riha, Karel; Mercier, Raphael

2016-01-01

Cell cycle control must be modified at meiosis to allow two divisions to follow a single round of DNA replication, resulting in ploidy reduction. The mechanisms that ensure meiosis termination at the end of the second and not at the end of first division are poorly understood. We show here that Arabidopsis thaliana TDM1, which has been previously shown to be essential for meiotic termination, interacts directly with the Anaphase-Promoting Complex. Further, mutations in TDM1 in a conserved putative Cyclin-Dependant Kinase (CDK) phosphorylation site (T16-P17) dominantly provoked premature meiosis termination after the first division, and the production of diploid spores and gametes. The CDKA;1-CYCA1.2/TAM complex, which is required to prevent premature meiotic exit, phosphorylated TDM1 at T16 in vitro. Finally, while CYCA1;2/TAM was previously shown to be expressed only at meiosis I, TDM1 is present throughout meiosis. These data, together with epistasis analysis, lead us to propose that TDM1 is an APC/C component whose function is to ensure meiosis termination at the end of meiosis II, and whose activity is inhibited at meiosis I by CDKA;1-TAM-mediated phosphorylation to prevent premature meiotic exit. This provides a molecular mechanism for the differential decision of performing an additional round of division, or not, at the end of meiosis I and II, respectively. PMID:26871453

Implications of Organizational Planning for Crisis Relocation.

DTIC Science & Technology

1982-12-01

AD-A 23 956 IMPLICATIONS OF ORGANIZATIDNAL PLANNING FOR CRISIS RELOCATION(U) NORTH CAROLINA DEPT OF CRIME CONTROL AND PUBLIC SAFETY RALEIG.. M A...policies of the Federal Emergency Management Agency. Division of Emergency Management North Carolina Department of Crime Control and Public Safety...North Carolina Department of Crime Control and Work Unit 0 4412 1 Public Safety, 116 W.Jones St. ,Raleigh, NC 27611 II. CONTROLLING OFFICE NAME AND

Gender, division of unpaid family work and psychological distress in dual-earner families.

PubMed

Tao, Wenting; Janzen, Bonnie L; Abonyi, Sylvia

2010-06-18

Epidemiological studies have only recently begun to address the consequences of unpaid family work (ie., housework and child rearing) for mental health. Although research is suggestive of an association between the division of unpaid family work and psychological health, especially for women, additional research is required to clarify the conditions under which such a relationship holds. The purpose of the present study was to examine more nuanced relationships between the division of family work and psychological distress by disaggregating the family work construct according to type (housework/child rearing), control over scheduling, and evaluations of fairness. Analysis of data obtained from a cross-sectional telephone survey conducted in a Canadian city. Analyses were based on 293 employed parents (182 mothers and 111 fathers), with at least one preschool child, living in dual-earner households. Several multiple linear regression models were estimated with psychological distress as the outcome, adjusting for confounders. For mothers, more perceived time spent in child rearing (particularly primary child care) and high-schedule-control housework tasks (e.g. yard work) relative to one's partner, were associated with greater distress. For fathers, perceived unfairness in the division of housework and child rearing were associated with greater distress. Although methodological limitations temper firm conclusions, these results suggest that the gendered nature of household work has implications for the psychological well-being of both mothers and fathers of preschool children in dual-earner households. However, more longitudinal research and the development of theoretically-informed measures of family work are needed to advance the field.

75 FR 57019 - Pesticide Product Registrations; Conditional Approval

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-17

... foliar use on soybeans to control brown spot, cercospora blight, frogeye leaf spot, leaf spot, powdery..., 2010. G. Jeffrey Herndon, Acting Director, Registration Division, Office of Pesticide Programs. [FR Doc...

10 CFR 95.9 - Communications.

Code of Federal Regulations, 2011 CFR

2011-01-01

...: (a) By mail addressed to: ATTN: Document Control Desk, Director, Division of Security Operations....gov/site-help/e-submittals.html; by e-mail to [email protected]; or by writing the Office of...

76 FR 3641 - National Cancer Institute; Notice of Closed Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-20

... Training Review Branch, Division of Extramural Activities, National Cancer Institute, 6116 Executive... Biology Research; 93.397, Cancer Centers Support; 93.398, Cancer Research Manpower; 93.399, Cancer Control...

Association of Employee Attributes and Exceptional Performance Rating at a National Center of the US Centers for Disease Control and Prevention, 2011.

PubMed

Roberts, Henry; Myles, Ranell L; Truman, Benedict I; Dean, Hazel D

2015-01-01

Employee performance evaluation motivates and rewards exceptional individual performance that advances the achievement of organizational goals. The Centers for Disease Control and Prevention (CDC) and its operating units evaluate employee performance annually and reward exceptional performance with a cash award or quality step increase in pay. A summary performance rating (SPR) of "exceptional" indicated personal achievements in 2011 that were beyond expectations described in the employee's performance plan. To determine whether personal attributes and job setting of civil service employees were associated with an exceptional SPR in National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention (NCHHSTP) in 2011. Data from the CDC 2011 performance management database collected in 2012 were analyzed in 2013 to identify SPR, personal attributes, and job settings of full-time civil service employees. Multivariate logistic regression controlled for confounding and stratified analysis detected effect modifiers of the association between receiving an exceptional SPR in 2011 and gender, race/ethnicity, education, job location, job series, grade level, years in grade, years of federal service, supervisory role, and NCHHSTP division. Among the 1037 employees, exceptional SPR was independently associated with: female gender (adjusted odds ratio: 1.7 [1.3, 2.3]), advanced degrees (doctorate: 1.7 [1.1, 2.5] master's: [1.1, 2.0]), headquarters location (2.8 [1.9, 4.1]), higher pay grade (3.3 [2.4, 4.5]) and years in grade (0-1 years: 1.7 [1.3, 2.4]; 2-4 years: 1.5 [1.1, 2.0]), division level (Division A: 5.0 [2.5, 9.9]; Division B: 5.5 [3.5, 8.8]), and supervisory status (at a lower-pay grade) (odds ratio: 3.7 [1.1, 11.3]). Exceptional SPR is independently associated with personal employee attributes and job settings that are not modifiable by interventions designed to improve employee performance based on accomplishments.

F-actin mechanics control spindle centring in the mouse zygote

NASA Astrophysics Data System (ADS)

Chaigne, Agathe; Campillo, Clément; Voituriez, Raphaël; Gov, Nir S.; Sykes, Cécile; Verlhac, Marie-Hélène; Terret, Marie-Emilie

2016-01-01

Mitotic spindle position relies on interactions between astral microtubules nucleated by centrosomes and a rigid cortex. Some cells, such as mouse oocytes, do not possess centrosomes and astral microtubules. These cells rely only on actin and on a soft cortex to position their spindle off-centre and undergo asymmetric divisions. While the first mouse embryonic division also occurs in the absence of centrosomes, it is symmetric and not much is known on how the spindle is positioned at the exact cell centre. Using interdisciplinary approaches, we demonstrate that zygotic spindle positioning follows a three-step process: (1) coarse centring of pronuclei relying on the dynamics of an F-actin/Myosin-Vb meshwork; (2) fine centring of the metaphase plate depending on a high cortical tension; (3) passive maintenance at the cell centre. Altogether, we show that F-actin-dependent mechanics operate the switch between asymmetric to symmetric division required at the oocyte to embryo transition.

The TCP4 transcription factor of Arabidopsis blocks cell division in yeast at G1→S transition.

PubMed

Aggarwal, Pooja; Padmanabhan, Bhavna; Bhat, Abhay; Sarvepalli, Kavitha; Sadhale, Parag P; Nath, Utpal

2011-07-01

The TCP transcription factors control important aspects of plant development. Members of class I TCP proteins promote cell cycle by regulating genes directly involved in cell proliferation. In contrast, members of class II TCP proteins repress cell division. While it has been postulated that class II proteins induce differentiation signal, their exact role on cell cycle has not been studied. Here, we report that TCP4, a class II TCP protein from Arabidopsis that repress cell proliferation in developing leaves, inhibits cell division by blocking G1→S transition in budding yeast. Cells expressing TCP4 protein with increased transcriptional activity fail to progress beyond G1 phase. By analyzing global transcriptional status of these cells, we show that expression of a number of cell cycle genes is altered. The possible mechanism of G1→S arrest is discussed. Copyright © 2011 Elsevier Inc. All rights reserved.

Substantial contribution of extrinsic risk factors to cancer development

PubMed Central

Wu, Song; Powers, Scott; Zhu, Wei; Hannun, Yusuf A

2015-01-01

Summary Recent research has highlighted a strong correlation between tissue-specific cancer risk and the lifetime number of tissue-specific stem cell divisions. Whether such correlation implies a high unavoidable intrinsic cancer risk has become a key public health debate with dissemination of the ‘bad luck’ hypothesis. Here we provide evidence that intrinsic risk factors contribute only modestly (<10~30%) to cancer development. First, we demonstrate that the correlation between stem-cell division and cancer risk does not distinguish between the effects of intrinsic and extrinsic factors. Next, we show that intrinsic risk is better estimated by the lower bound risk controlling for total stem cell divisions. Finally, we show that the rates of endogenous mutation accumulation by intrinsic processes are not sufficient to account for the observed cancer risks. Collectively, we conclude that cancer risk is heavily influenced by extrinsic factors. These results carry immense consequences for strategizing cancer prevention, research, and public health. PMID:26675728

Identification of a new stem cell population that generates Drosophila flight muscles.

PubMed

Gunage, Rajesh D; Reichert, Heinrich; VijayRaghavan, K

2014-08-18

How myoblast populations are regulated for the formation of muscles of different sizes is an essentially unanswered question. The large flight muscles of Drosophila develop from adult muscle progenitor (AMP) cells set-aside embryonically. The thoracic segments are all allotted the same small AMP number, while those associated with the wing-disc proliferate extensively to give rise to over 2500 myoblasts. An initial amplification occurs through symmetric divisions and is followed by a switch to asymmetric divisions in which the AMPs self-renew and generate post-mitotic myoblasts. Notch signaling controls the initial amplification of AMPs, while the switch to asymmetric division additionally requires Wingless, which regulates Numb expression in the AMP lineage. In both cases, the epidermal tissue of the wing imaginal disc acts as a niche expressing the ligands Serrate and Wingless. The disc-associated AMPs are a novel muscle stem cell population that orchestrates the early phases of adult flight muscle development.

CDC's Cervical Cancer Study

MedlinePlus

... Radon What We Know What States Can Do Case Study: Reducing Radon in Illinois What Comprehensive Cancer Control ... reviewed: July 9, 2015 Page last updated: December 1, 2015 Content source: Division of Cancer Prevention and ...

78 FR 26056 - National Cancer Institute; Notice of Closed Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-03

... Cancer Institute, Division of Extramural Activities, Research Programs Review Branch, 9609 Medical Center... Biology Research; 93.397, Cancer Centers Support; 93.398, Cancer Research Manpower; 93.399, Cancer Control...

78 FR 26055 - National Cancer Institute; Notice of Closed Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-03

... Logistics Branch, Division of Extramural Activities, National Cancer Institute, NIH, 9609 Medical Center... Biology Research; 93.397, Cancer Centers Support; 93.398, Cancer Research Manpower; 93.399, Cancer Control...

75 FR 16706 - Proposed Significant New Use Rule for 1-Propene, 2,3,3,3-tetrafluoro-

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-02

... section 5(a)(2) of the Toxic Substances Control Act (TSCA) for the chemical substance identified as 1... address: [email protected] . For technical information contact: Kenneth Moss, Chemical Control Division... be potentially affected by this action if you manufacture, import, process, or use the chemical...

46 CFR 127.225 - Structural fire protection.

Code of Federal Regulations, 2014 CFR

2014-10-01

... wheelhouses, containing accommodation, service and control spaces, facing the cargo area must be constructed of steel and comply with §§ 32.56-20, 32.56-21, and 32.56-22 of this chapter. (d) Cargo pump rooms must be separated from accommodation spaces, service spaces, and control stations by A-60 divisions. (e...

CPFP Summer Curriculum: Principles and Practices of Cancer Prevention and Control Course | Division of Cancer Prevention

Cancer.gov

This Cancer Prevention Fellowship Program four-week summer course provides specialized instruction in the principles and practice of cancer prevention and control. Participants will gain a broad-based perspective on concepts, issues, and applications related to this field. The course typically covers the following topics: |

Board of Pesticides Control: Maine ACF

Science.gov Websites

| Search Maine.gov Department of Agriculture, Conservation and Forestry Contact Us | Get Email/SMS Updates ; Programs → Bureau of Agriculture → Division of Animal and Plant Health → Board of Pesticides Control Plates Outdoor Heritage Fund Lottery Ticket Donations & More Contact Department of Agriculture

Citrus Quality Control: An NMR/MRI Problem-Based Experiment

ERIC Educational Resources Information Center

Erhart, Sarah E.; McCarrick, Robert M.; Lorigan, Gary A.; Yezierski, Ellen J.

2016-01-01

An experiment seated in an industrial context can provide an engaging framework and unique learning opportunity for an upper-division physical chemistry laboratory. An experiment that teaches NMR/MRI through a problem-based quality control of citrus products was developed. In this experiment, using a problem-based learning (PBL) approach, students…

Instructional Choices in Language Arts: Reality or Illusion.

ERIC Educational Resources Information Center

Hensley, Robin

A first-year first grade teacher's professional disappointments were broken down into three divisions: (1) power and control, or the lack of them; (2) co-worker socialization; and (3) lack of professionalism and support. Lack of control was reflected in pressure from administrators to use certain texts, or even to decorate classrooms in a certain…

76 FR 33302 - Proposed Data Collections Submitted for Public Comment and Recommendations

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-08

... disease, including cancer, and death. The only proven strategy for reducing the risk of tobacco- related... Comprehensive Cancer Control and Tobacco Control Program Partnerships -- New -- Division of Cancer Prevention... cause of death in the United States, causing over 443,000 deaths each year and resulting in an annual...

75 FR 26962 - Agency Information Collection Activities; Submission for OMB Review; Comment Request; Correction

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-13

... Rule), 16 CFR part 315. The document contained an incorrect OMB Control No. for the pre-existing..., Attorney, Division of Advertising Practices, Bureau of Consumer Protection, Federal Trade Commission, 600... comment while seeking OMB approval to renew the pre-existing clearance for the Rule (OMB Control No. 3084...

Collaborative Tool for Command and Control Team Effectiveness Studies: Experimental Test of Interventions to Improve Performance in Command and Control

DTIC Science & Technology

2008-11-01

weapons system in the United States Air Force (USAF) inventory (Garrity, Morley, Rodriguez , & Tossell, 2004). It is important for potential AOC operators...Research Division in Mesa , AZ. Additionally, cadets were able to participate in two conferences. Please see conference report at Attachment 3

Cancer Prevention Fellowship Program (CPFP) | Division of Cancer Prevention

Cancer.gov

The Cancer Prevention Fellowship provides a strong foundation for scientists and clinicians to train in the field of cancer prevention and control. This structured, multidisciplinary program offers early career scientists from different health disciplines a variety of postdoctoral training opportunities . | Training to form a strong foundation in cancer prevention and control

Model transformations for state-space self-tuning control of multivariable stochastic systems

NASA Technical Reports Server (NTRS)

Shieh, Leang S.; Bao, Yuan L.; Coleman, Norman P.

1988-01-01

The design of self-tuning controllers for multivariable stochastic systems is considered analytically. A long-division technique for finding the similarity transformation matrix and transforming the estimated left MFD to the right MFD is developed; the derivation is given in detail, and the procedures involved are briefly characterized.

Legal Education in Great Britain. Bulletin, 1915, No. 18. Whole Number 643

ERIC Educational Resources Information Center

Richards, H. S.

1915-01-01

Admission to the legal profession in England is controlled by organizations representing the solicitors and barristers, the two great divisions into which the profession is divided. this control by private organizations is peculiar to England, and a proper appreciation of the present condition and tendencies in English legal education therefore…

H. W. Laboratory manual: 100 Area section

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1950-07-01

The purpose of this manual is to present a Hazard Breakdown of all jobs normally encountered in the laboratory work of the three sections comprising the Analytic Section, Metallurgy and Control Division of the Technical Department. A Hazard Breakdown is a careful analysis of any job in which the source of possible dangers is clearly indicated for each particular step. The analysis is prepared by individuals who are thoroughly familiar with the specific job or procedure. It is felt that if the hazards herein outlined are recognized by the Laboratory personnel and the suggested safety cautions followed, the chance formore » injury will be minimized and the worker will become generally more safety conscious. The manual, which is prefaced by the general safety rules applying to all the laboratories, is divided into three main sections, one for each of the three sections into which the Laboratories Division is divided. These sections are as follows: Section 1 -- 200 Area Control; Section 2 -- 100 Area Control; Section 3 -- 300 Area Control, Essential Materials, and Methods Improvement.« less

Bacterial size matters: Multiple mechanisms controlling septum cleavage and diplococcus formation are critical for the virulence of the opportunistic pathogen Enterococcus faecalis

PubMed Central

Salamaga, Bartłomiej; Prajsnar, Tomasz K.; Willemse, Joost; Bewley, Martin A.; Chau, Françoise

2017-01-01

Enterococcus faecalis is an opportunistic pathogen frequently isolated in clinical settings. This organism is intrinsically resistant to several clinically relevant antibiotics and can transfer resistance to other pathogens. Although E. faecalis has emerged as a major nosocomial pathogen, the mechanisms underlying the virulence of this organism remain elusive. We studied the regulation of daughter cell separation during growth and explored the impact of this process on pathogenesis. We demonstrate that the activity of the AtlA peptidoglycan hydrolase, an enzyme dedicated to septum cleavage, is controlled by several mechanisms, including glycosylation and recognition of the peptidoglycan substrate. We show that the long cell chains of E. faecalis mutants are more susceptible to phagocytosis and are no longer able to cause lethality in the zebrafish model of infection. Altogether, this work indicates that control of cell separation during division underpins the pathogenesis of E. faecalis infections and represents a novel enterococcal virulence factor. We propose that inhibition of septum cleavage during division represents an attractive therapeutic strategy to control infections. PMID:28742152

Case-control study on uveal melanoma (RIFA): rational and design

PubMed Central

Schmidt-Pokrzywniak, Andrea; Jöckel, Karl-Heinz; Bornfeld, Norbert; Stang, Andreas

2004-01-01

Background Although a rare disease, uveal melanoma is the most common primary intraocular malignancy in adults, with an incidence rate of up to 1.0 per 100,000 persons per year in Europe. Only a few consistent risk factors have been identified for this disease. We present the study design of an ongoing incident case-control study on uveal melanoma (acronym: RIFA study) that focuses on radiofrequency radiation as transmitted by radio sets and wireless telephones, occupational risk factors, phenotypical characteristics, and UV radiation. Methods/Design We conduct a case-control study to identify the role of different exposures in the development of uveal melanoma. The cases of uveal melanoma were identified at the Division of Ophthalmology, University of Essen, a referral centre for tumours of the eye. We recruit three control groups: population controls, controls sampled from those ophthalmologists who referred cases to the Division of Ophthalmology, University of Duisburg-Essen, and sibling controls. For each case the controls are matched on sex and age (five year groups), except for sibling controls. The data are collected from the study participants by short self-administered questionnaire and by telephone interview. During and at the end of the field phase, the data are quality-checked. To estimate the effect of exposures on uveal melanoma risk, we will use conditional logistic regression that accounts for the matching factors and allows to control for potential confounding. PMID:15318944

Long-term, high-resolution confocal time lapse imaging of Arabidopsis cotyledon epidermis during germination.

PubMed

Peterson, Kylee M; Torii, Keiko U

2012-12-31

Imaging in vivo dynamics of cellular behavior throughout a developmental sequence can be a powerful technique for understanding the mechanics of tissue patterning. During animal development, key cell proliferation and patterning events occur very quickly. For instance, in Caenorhabditis elegans all cell divisions required for the larval body plan are completed within six hours after fertilization, with seven mitotic cycles(1); the sixteen or more mitoses of Drosophila embryogenesis occur in less than 24 hr(2). In contrast, cell divisions during plant development are slow, typically on the order of a day (3,4,5) . This imposes a unique challenge and a need for long-term live imaging for documenting dynamic behaviors of cell division and differentiation events during plant organogenesis. Arabidopsis epidermis is an excellent model system for investigating signaling, cell fate, and development in plants. In the cotyledon, this tissue consists of air- and water-resistant pavement cells interspersed with evenly distributed stomata, valves that open and close to control gas exchange and water loss. Proper spacing of these stomata is critical to their function, and their development follows a sequence of asymmetric division and cell differentiation steps to produce the organized epidermis (Fig. 1). This protocol allows observation of cells and proteins in the epidermis over several days of development. This time frame enables precise documentation of stem-cell divisions and differentiation of epidermal cells, including stomata and epidermal pavement cells. Fluorescent proteins can be fused to proteins of interest to assess their dynamics during cell division and differentiation processes. This technique allows us to understand the localization of a novel protein, POLAR(6), during the proliferation stage of stomatal-lineage cells in the Arabidopsis cotyledon epidermis, where it is expressed in cells preceding asymmetric division events and moves to a characteristic area of the cell cortex shortly before division occurs. Images can be registered and streamlined video easily produced using public domain software to visualize dynamic protein localization and cell types as they change over time.

Reward value-based gain control: divisive normalization in parietal cortex.

PubMed

Louie, Kenway; Grattan, Lauren E; Glimcher, Paul W

2011-07-20

The representation of value is a critical component of decision making. Rational choice theory assumes that options are assigned absolute values, independent of the value or existence of other alternatives. However, context-dependent choice behavior in both animals and humans violates this assumption, suggesting that biological decision processes rely on comparative evaluation. Here we show that neurons in the monkey lateral intraparietal cortex encode a relative form of saccadic value, explicitly dependent on the values of the other available alternatives. Analogous to extra-classical receptive field effects in visual cortex, this relative representation incorporates target values outside the response field and is observed in both stimulus-driven activity and baseline firing rates. This context-dependent modulation is precisely described by divisive normalization, indicating that this standard form of sensory gain control may be a general mechanism of cortical computation. Such normalization in decision circuits effectively implements an adaptive gain control for value coding and provides a possible mechanistic basis for behavioral context-dependent violations of rationality.

Ovarian control of nectar collection in the honey bee (Apis mellifera).

PubMed

Siegel, Adam J; Freedman, Colin; Page, Robert E

2012-01-01

Honey bees are a model system for the study of division of labor. Worker bees demonstrate a foraging division of labor (DOL) by biasing collection towards carbohydrates (nectar) or protein (pollen). The Reproductive ground-plan hypothesis of Amdam et al. proposes that foraging DOL is regulated by the networks that controlled foraging behavior during the reproductive life cycle of honey bee ancestors. Here we test a proposed mechanism through which the ovary of the facultatively sterile worker impacts foraging bias. The proposed mechanism suggests that the ovary has a regulatory effect on sucrose sensitivity, and sucrose sensitivity impacts nectar loading. We tested this mechanism by measuring worker ovary size (ovariole number), sucrose sensitivity, and sucrose solution load size collected from a rate-controlled artificial feeder. We found a significant interaction between ovariole number and sucrose sensitivity on sucrose solution load size when using low concentration nectar. This supports our proposed mechanism. As nectar and pollen loading are not independent, a mechanism impacting nectar load size would also impact pollen load size.

NPS Government Purchase Card Program: An Analysis of Internal Controls

DTIC Science & Technology

2014-03-01

approving official APC agency program coordinator CCPMD Consolidated Card Program Management Division CH cardholder COSO Committee of Sponsoring...correct, and minimize fraud, waste, and abuse” (DPAP, 2011, p. 2-2). To minimize risks , the management and internal controls should have support from...three interrelated subjects: enterprise risk management (ERM), internal control, and fraud deterrence” (para. 6). The 23 five components of an

American British Canadian, Australian Armies Standardization Program Catalog of War Games

DTIC Science & Technology

1991-05-21

Defense Netwrrk (ADNET), Command, Control Communication, Intelligence, Prioritization (C3IP) Links, FM2 Grid (read-in optimization) in Terrain Database...at a given echelon such as division and below. Force level models may be interactive with players performing various cnmard, control , and staff...and may require compliance with appropriate configuration control procedures. Also, release to contractors may be prohibited. FOR THE WASHINGTON

Performance analysis of Integrated Communication and Control System networks

NASA Technical Reports Server (NTRS)

Halevi, Y.; Ray, A.

1990-01-01

This paper presents statistical analysis of delays in Integrated Communication and Control System (ICCS) networks that are based on asynchronous time-division multiplexing. The models are obtained in closed form for analyzing control systems with randomly varying delays. The results of this research are applicable to ICCS design for complex dynamical processes like advanced aircraft and spacecraft, autonomous manufacturing plants, and chemical and processing plants.

Monitor and Control of the Deep-Space network via Secure Web

NASA Technical Reports Server (NTRS)

Lamarra, N.

1997-01-01

(view graph) NASA lead center for robotic space exploration. Operating division of Caltech/Jet Propulsion Laboratory. Current missions, Voyagers, Galileo, Pathfinder, Global Surveyor. Upcoming missions, Cassini, Mars and New Millennium.

78 FR 19605 - Greenhouse Gas Reporting Rule: Revision to Best Available Monitoring Method Request Submission...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-02

..., Administrative practice and procedures, Air pollution control, Greenhouse gases, Monitoring, Reporting and..., 2013. FOR FURTHER INFORMATION CONTACT: Carole Cook, Climate Change Division, Office of Atmospheric...

Advanced Control Techniques with Fuzzy Logic

DTIC Science & Technology

2014-06-01

ORGANIZATION Structural Validation Branch ( AFRL /RQVV) Aerospace Vehicles Division Air Force Research Laboratory , Aerospace Systems Directorate......SPONSORING/MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSORING/MONITORING Air Force Research Laboratory Aerospace Systems Directorate Wright

OVERVIEW: CCL PATHOGENS RESEARCH AT NRMRL

EPA Science Inventory

The Microbial Contaminants Control Branch (MCCB), Water Supply and Water Resources Division, National Risk Management Research Laboratory, conducts research on microbiological problems associated with source water quality, treatment processes, distribution and storage of drin...

78 FR 78967 - Agency Information Collection Activities: Proposed Collection; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-27

... Regulatory Affairs, Division of Regulations Development, Attention: Document Identifier/OMB Control Number... State Medicaid Programs; Use: In accordance with the Deficit Act of 2005, states are required to provide...

75 FR 51462 - Agency Information Collection Activities: Proposed Collection; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-20

... Submitting Drug Identifying Information to State Medicaid Programs; Use: Section 6002 of the Deficit..., Division of Regulations Development, Attention: Document Identifier/OMB Control Number, Room C4-26-05, 7500...

10 CFR 75.35 - Material status reports.

Code of Federal Regulations, 2010 CFR

2010-01-01

... physical inventory which is taken as part of the material accounting and control procedures required by... instructions may be obtained from the U.S. Nuclear Regulatory Commission, Division of Fuel Cycle Safety and...

40 CFR 62.6875 - Identification of plan-negative declaration.

Code of Federal Regulations, 2010 CFR

2010-07-01

...—negative declaration. Letter from the Chief of the Air Pollution Control Division of the Department of... in the State of Nebraska. Total Reduced Sulfur Emissions From Existing Kraft Pulp Mills ...

40 CFR 62.6880 - Identification of plan-negative declaration.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Nebraska Total Reduced Sulfur Emissions from Existing Kraft Pulp Mills § 62.6880 Identification of plan—negative declaration. Letter from the Chief of the Air Pollution Control Division of the Department of...

76 FR 3157 - Joint Operations Center Relocation Project, Sacramento County, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-19

... Water Resources (DWR), the lead State agency, will prepare a joint EIS/EIR for the proposed Joint... following departments: DWR's Division of Operations and Maintenance, Operations Control Office, State Water...

10 CFR 12.310 - Payment of award.

Code of Federal Regulations, 2010 CFR

2010-01-01

... award, accompanied by a certification that the applicant will not seek review of the decision in the... submission to the Director, Division of Accounting and Finance, Office of the Controller, U.S. Nuclear...

Controlling Cholesterol with Statins

MedlinePlus

... already in the blood. According to James P. Smith, M.D., M.S., deputy director of the Division ... the risk of heart disease and stroke,” says Smith. “But other factors that are out of our ...

Systemic control of cell division and endoreduplication by NAA and BAP by modulating CDKs in root tip cells of Allium cepa.

PubMed

Tank, Jigna G; Thaker, Vrinda S

2014-01-01

Molecular mechanism regulated by auxin and cytokinin during endoreduplication, cell division, and elongation process is studied by using Allium cepa roots as a model system. The activity of CDK genes modulated by auxin and cytokinin during cell division, elongation, and endoreduplication process is explained in this research work. To study the significance of auxin and cytokinin in the management of cell division and endoreduplication process in plant meristematic cells at molecular level endoreduplication was developed in root tips of Allium cepa by giving colchicine treatment. There were inhibition of vegetative growth, formation of c-tumor at root tip, and development of endoreduplicated cells after colchicine treatment. This c-tumor was further treated with NAA and BAP to reinitiate vegetative growth in roots. BAP gave positive response in reinitiation of vegetative growth of roots from center of c-tumor. However, NAA gave negative response in reinitiation of vegetative growth of roots from c-tumor. Further, CDKs gene expression analysis from normal, endoreduplicated, and phytohormone (NAA or BAP) treated root tip was done and remarkable changes in transcription level of CDK genes in normal, endoreduplicated, and phytohormones treated cells were observed.

Multiple access techniques and spectrum utilization of the GLOBALSTAR mobile satellite system

NASA Astrophysics Data System (ADS)

Louie, Ming; Cohen, Michel; Rouffet, Denis; Gilhousen, Klein S.

The GLOBALSTAR System is a Low Earth Orbit (LEO) satellite-based mobile communications system that is interoperable with the current and future Public Land Mobile Network (PLMN). The GLOBALSTAR System concept is based upon technological advancement in two key areas: (1) the advancement in LEO satellite technology; (2) the advancement in cellular telephone technology, including the commercial applications of Code Division Multiple Access (CDMA) technologies, and of the most recent progress in Time Division Multiple Access technologies. The GLOBALSTAR System uses elements of CDMA, Frequency Division Multiple Access (FDMA), and Time Division Multiple Access (TDMA) technology, combining with satellite Multiple Beam Antenna (MBA) technology, to arrive at one of the most efficient modulation and multiple access system ever proposed for a satellite communications system. The technology used in GLOBALSTAR exploits the following techniques in obtaining high spectral efficiency and affordable cost per channel, with minimum coordination among different systems: power control, in open and closed loops, voice activation, spot beam satellite antenna for frequency reuse, weighted satellite antenna gain, multiple satellite coverage, and handoff between satellites. The GLOBALSTAR system design will use the following frequency bands: 1610-1626.5 MHz for up-link and 2483.5-2500 MHz for down-link.

Systemic Control of Cell Division and Endoreduplication by NAA and BAP by Modulating CDKs in Root Tip Cells of Allium cepa

PubMed Central

Tank, Jigna G.; Thaker, Vrinda S.

2014-01-01

Molecular mechanism regulated by auxin and cytokinin during endoreduplication, cell division, and elongation process is studied by using Allium cepa roots as a model system. The activity of CDK genes modulated by auxin and cytokinin during cell division, elongation, and endoreduplication process is explained in this research work. To study the significance of auxin and cytokinin in the management of cell division and endoreduplication process in plant meristematic cells at molecular level endoreduplication was developed in root tips of Allium cepa by giving colchicine treatment. There were inhibition of vegetative growth, formation of c-tumor at root tip, and development of endoreduplicated cells after colchicine treatment. This c-tumor was further treated with NAA and BAP to reinitiate vegetative growth in roots. BAP gave positive response in reinitiation of vegetative growth of roots from center of c-tumor. However, NAA gave negative response in reinitiation of vegetative growth of roots from c-tumor. Further, CDKs gene expression analysis from normal, endoreduplicated, and phytohormone (NAA or BAP) treated root tip was done and remarkable changes in transcription level of CDK genes in normal, endoreduplicated, and phytohormones treated cells were observed. PMID:24955358

High-Explosives Applications Facility (HEAF)

NASA Astrophysics Data System (ADS)

Morse, J. L.; Weingart, R. C.

1989-03-01

This Safety Analysis Report (SAR) reviews the safety and environmental aspects of the High Explosives Applications Facility (HEAF). Topics covered include the site selected for the HEAF, safety design criteria, operations planned within the facility, and the safety and environmental analyses performed on this project to date. Provided in the Summary section is a review of hazards and the analyses, conclusions, and operating limits developed in this SAR. Appendices provide supporting documents relating to this SAR. This SAR is required by the LLNL Health and Safety Manual and DOE Order 5481.1B(2) to document the safety analysis efforts. The SAR was assembled by the Hazards Control Department, B-Division, and HEAF project personnel. This document was reviewed by B Division, the Chemistry Department, the Hazards Control Department, the Laboratory Associate Director for Administration and Operations, and the Associate Directors ultimately responsible for HEAF operations.

In tobacco BY-2 cells xyloglucan oligosaccharides alter the expression of genes involved in cell wall metabolism, signalling, stress responses, cell division and transcriptional control.

PubMed

González-Pérez, Lien; Perrotta, Lara; Acosta, Alexis; Orellana, Esteban; Spadafora, Natasha; Bruno, Leonardo; Bitonti, Beatrice M; Albani, Diego; Cabrera, Juan Carlos; Francis, Dennis; Rogers, Hilary J

2014-10-01

Xyloglucan oligosaccharides (XGOs) are breakdown products of XGs, the most abundant hemicelluloses of the primary cell walls of non-Poalean species. Treatment of cell cultures or whole plants with XGOs results in accelerated cell elongation and cell division, changes in primary root growth, and a stimulation of defence responses. They may therefore act as signalling molecules regulating plant growth and development. Previous work suggests an interaction with auxins and effects on cell wall loosening, however their mode of action is not fully understood. The effect of an XGO extract from tamarind (Tamarindus indica) on global gene expression was therefore investigated in tobacco BY-2 cells using microarrays. Over 500 genes were differentially regulated with similar numbers and functional classes of genes up- and down-regulated, indicating a complex interaction with the cellular machinery. Up-regulation of a putative XG endotransglycosylase/hydrolase-related (XTH) gene supports the mechanism of XGO action through cell wall loosening. Differential expression of defence-related genes supports a role for XGOs as elicitors. Changes in the expression of genes related to mitotic control and differentiation also support previous work showing that XGOs are mitotic inducers. XGOs also affected expression of several receptor-like kinase genes and transcription factors. Hence, XGOs have significant effects on expression of genes related to cell wall metabolism, signalling, stress responses, cell division and transcriptional control.

Substrate-specific regulation of ubiquitination by the anaphase-promoting complex

PubMed Central

Song, Ling

2011-01-01

By orchestrating the sequential degradation of a large number of cell cycle regulators, the ubiquitin ligase anaphase-promoting complex (APC/C) is essential for proliferation in all eukaryotes. The correct timing of APC/C-dependent substrate degradation, a critical feature of progression through mitosis, was long known to be controlled by mechanisms targeting the core APC/C-machinery. Recent experiments, however have revealed an important contribution of substrate-specific regulation of the APC/C to achieve accurate cell division. In this perspective, we describe different mechanisms of substrate-specific APC/C-regulation and discuss their importance for cell division. PMID:21191176

[Effect of ectopic expression of NtEXPA5 gene on cell size and growth of organs of transgenic tobacco plants].

PubMed

Kuluev, B R; Safiullina, M G; Kniazev, A V; Chemeris, A V

2013-01-01

We obtained transgenic tobacco plants demonstrating overexpression of NtEXPA5 gene that encodes alpha-expansin of Nicotiana tabacum. The transgenic plants were characterized by increased size of leaves and stems. However, size of flowers remained almost unchanged. The increase of organ sizes was induced by cell stretching only. Moreover, the number of cell divisions was even decreased. The obtained data suggest tight interaction between cell stretching regulation and cell division, which together provide the basic mechanism aimed at the controlling of plant organ sizes.

Integrated Safety Analysis Tiers

NASA Technical Reports Server (NTRS)

Shackelford, Carla; McNairy, Lisa; Wetherholt, Jon

2009-01-01

Commercial partnerships and organizational constraints, combined with complex systems, may lead to division of hazard analysis across organizations. This division could cause important hazards to be overlooked, causes to be missed, controls for a hazard to be incomplete, or verifications to be inefficient. Each organization s team must understand at least one level beyond the interface sufficiently enough to comprehend integrated hazards. This paper will discuss various ways to properly divide analysis among organizations. The Ares I launch vehicle integrated safety analyses effort will be utilized to illustrate an approach that addresses the key issues and concerns arising from multiple analysis responsibilities.

Low-latency optical parallel adder based on a binary decision diagram with wavelength division multiplexing scheme

NASA Astrophysics Data System (ADS)

Shinya, A.; Ishihara, T.; Inoue, K.; Nozaki, K.; Kita, S.; Notomi, M.

2018-02-01

We propose an optical parallel adder based on a binary decision diagram that can calculate simply by propagating light through electrically controlled optical pass gates. The CARRY and CARRY operations are multiplexed in one circuit by a wavelength division multiplexing scheme to reduce the number of optical elements, and only a single gate constitutes the critical path for one digit calculation. The processing time reaches picoseconds per digit when we use a 100-μm-long optical path gates, which is ten times faster than a CMOS circuit.

Characterization of a Null Allelic Mutant of the Rice NAL1 Gene Reveals Its Role in Regulating Cell Division

PubMed Central

Jiang, Dan; Fang, Jingjing; Lou, Lamei; Zhao, Jinfeng; Yuan, Shoujiang; Yin, Liang; Sun, Wei; Peng, Lixiang; Guo, Baotai; Li, Xueyong

2015-01-01

Leaf morphology is closely associated with cell division. In rice, mutations in Narrow leaf 1 (NAL1) show narrow leaf phenotypes. Previous studies have shown that NAL1 plays a role in regulating vein patterning and increasing grain yield in indica cultivars, but its role in leaf growth and development remains unknown. In this report, we characterized two allelic mutants of NARROW LEAF1 (NAL1), nal1-2 and nal1-3, both of which showed a 50% reduction in leaf width and length, as well as a dwarf culm. Longitudinal and transverse histological analyses of leaves and internodes revealed that cell division was suppressed in the anticlinal orientation but enhanced in the periclinal orientation in the mutants, while cell size remained unaltered. In addition to defects in cell proliferation, the mutants showed abnormal midrib in leaves. Map-based cloning revealed that nal1-2 is a null allelic mutant of NAL1 since both the whole promoter and a 404-bp fragment in the first exon of NAL1 were deleted, and that a 6-bp fragment was deleted in the mutant nal1-3. We demonstrated that NAL1 functions in the regulation of cell division as early as during leaf primordia initiation. The altered transcript level of G1- and S-phase-specific genes suggested that NAL1 affects cell cycle regulation. Heterogenous expression of NAL1 in fission yeast (Schizosaccharomyces pombe) further supported that NAL1 affects cell division. These results suggest that NAL1 controls leaf width and plant height through its effects on cell division. PMID:25658704

Time to Stabilization of Anterior Cruciate Ligament–Reconstructed Versus Healthy Knees in National Collegiate Athletic Association Division I Female Athletes

PubMed Central

Webster, Kathryn A.; Gribble, Phillip A.

2010-01-01

Abstract Context: Jump landing is a common activity in collegiate activities, such as women's basketball, volleyball, and soccer, and is a common mechanism for anterior cruciate ligament (ACL) injury. It is important to better understand how athletes returning to competition after ACL reconstruction are able to maintain dynamic postural control during a jump landing. Objective: To use time to stabilization (TTS) to measure differences in dynamic postural control during jump landing in ACL-reconstructed (ACLR) knees compared with healthy knees among National Collegiate Athletic Association Division I female athletes. Design: Case-control study. Setting: University athletic training research laboratory. Patients or Other Participants: Twenty-four Division I female basketball, volleyball, and soccer players volunteered and were assigned to the healthy control group (n  =  12) or the ACLR knee group (n  =  12). Participants with ACLR knees were matched to participants with healthy knees by sport and by similar age, height, and mass. Intervention(s): At 1 session, participants performed a single-leg landing task for both limbs. They were instructed to stabilize as quickly as possible in a single-limb stance and remain as motionless as possible for 10 seconds. Main Outcome Measure(s): The anterior-posterior TTS and medial-lateral TTS ground reaction force data were used to calculate resultant vector of the TTS (RVTTS) during a jump landing. A 1-way analysis of variance was used to determine group differences on RVTTS. The means and SDs from the participants' 10 trials in each leg were used for the analyses. Results: The ACLR group (2.01 ± 0.15 seconds, 95% confidence interval [CI]  =  1.91, 2.10) took longer to stabilize than the control group (1.90 ± 0.07 seconds, 95% CI  =  1.86, 1.95) (F1,22  =  4.28, P  =  .05). This result was associated with a large effect size and a 95% CI that did not cross zero (Cohen d  =  1.0, 95% CI  =  0.91, 1.09). Conclusions: Although they were Division I female athletes at an average of 2.5 years after ACL reconstruction, participants with ACLR knees demonstrated dynamic postural-control deficits as evidenced by their difficulty in controlling ground reaction forces. This increased TTS measurement might contribute to the established literature reflecting differences in single-limb dynamic control. Clinicians might need to focus rehabilitation efforts on stabilization after jump landing. Further research is needed to determine if TTS is a contributing factor in future injury. PMID:21062181

Hounsfield unit change in root and alveolar bone during canine retraction.

PubMed

Jiang, Feifei; Liu, Sean S-Y; Xia, Zeyang; Li, Shuning; Chen, Jie; Kula, Katherine S; Eckert, George

2015-04-01

The objective of this study was to determine the Hounsfield unit (HU) changes in the alveolar bone and root surfaces during controlled canine retractions. Eighteen maxillary canine retraction patients were selected for this split-mouth design clinical trial. The canines in each patient were randomly assigned to receive either translation or controlled tipping treatment. Pretreatment and posttreatment cone-beam computed tomography scans of each patient were used to determine tooth movement direction and HU changes. The alveolar bone and root surface were divided into 108 divisions, respectively. The HUs in each division were measured. Mixed-model analysis of variance was applied to test the HU change distribution at the P <0.05 significance level. The HU changes varied with the directions relative to the canine movement. The HU reductions occurred at the root surfaces. Larger reductions occurred in the divisions that were perpendicular to the moving direction. However, HUs decreased in the alveolar bone in the moving direction. The highest HU reduction was at the coronal level. HU reduction occurs on the root surface in the direction perpendicular to tooth movement and in the alveolar bone in the direction of tooth movement when a canine is retracted. Copyright © 2015 American Association of Orthodontists. Published by Elsevier Inc. All rights reserved.

Successful Treatment of Postpeak Stage Patients with Class II Division 1 Malocclusion Using Non-extraction and Multiloop Edgewise Archwire Therapy: A Report on 16 Cases

PubMed Central

Liu, Jun; Zou, Ling; Zhao, Zhi-he; Welburn, Neala; Yang, Pu; Tang, Tian; Li, Yu

2009-01-01

Aim To determine cephalometrically the mechanism of the treatment effects of non-extraction and multiloop edgewise archwire (MEAW) technique on postpeak Class II Division 1 patients. Methodology In this retrospective study, 16 postpeak Class II Division 1 patients successfully corrected using a non-extraction and MEAW technique were cephalometrically evaluated and compared with 16 matched control subjects treated using an extraction technique. Using CorelDRAW® software, standardized digital cephalograms pre- and post-active treatments were traced and a reference grid was set up. The superimpositions were based on the cranial base, the mandibular and the maxilla regions,and skeletal and dental changes were measured. Changes following treatment were evaluated using the paired-sample t-test. Student's t-test for unpaired samples was used to assess the differences in changes between the MEAW and the extraction control groups. Results The correction of the molar relationships comprised 54% skeletal change (mainly the advancement of the mandible) and 46% dental change. Correction of the anterior teeth relationships comprised 30% skeletal change and 70% dental change. Conclusion The MEAW technique can produce the desired vertical and sagittal movement of the tooth segment and then effectively stimulate mandibular advancement by utilizing the residual growth potential of the condyle. PMID:20690424

Hounsfield Unit Change in Root and Alveolar Bone during Canine Retraction

PubMed Central

Jiang, Feifei; Liu, Sean Y.; Xia, Zeyang; Li, Shuning; Chen, Jie; Kula, Katherine S.; Eckert, George

2014-01-01

Objectives The objective of this study was to determine the Hounsfield unit (HU) changes in the alveolar bone and root surface during controlled canine retractions. Methods Eighteen maxillary canine retraction patients were selected for this split mouth design clinical trial. The canines in each patient were randomly assigned to receive either translation or controlled tipping treatment strategy. Pre- and post-treatment cone beam computed tomography scans of each patient were used to determine tooth movement direction and HU changes. The alveolar bone and root surface were divided into 108 divisions, respectively. The HU in each division was measured. The Mixed-model ANOVA was applied to test the HU change distribution at the p<0.05 significant level. Results The HU changes varied with the directions relative to the canine movement. The HU reduction occurred at the root surface. Larger reductions occurred in the divisions that were perpendicular to the moving direction. However, HU decreased in the alveolar bone in the moving direction. The highest HU reduction was at the coronal level. Conclusions HU reduction occurs on the root surface in the direction perpendicular to the tooth movement and in the alveolar bone in the direction of tooth movement when a canine is retracted. PMID:25836004

Self-organizing feature maps for dynamic control of radio resources in CDMA microcellular networks

NASA Astrophysics Data System (ADS)

Hortos, William S.

1998-03-01

The application of artificial neural networks to the channel assignment problem for cellular code-division multiple access (CDMA) cellular networks has previously been investigated. CDMA takes advantage of voice activity and spatial isolation because its capacity is only interference limited, unlike time-division multiple access (TDMA) and frequency-division multiple access (FDMA) where capacities are bandwidth-limited. Any reduction in interference in CDMA translates linearly into increased capacity. To satisfy the high demands for new services and improved connectivity for mobile communications, microcellular and picocellular systems are being introduced. For these systems, there is a need to develop robust and efficient management procedures for the allocation of power and spectrum to maximize radio capacity. Topology-conserving mappings play an important role in the biological processing of sensory inputs. The same principles underlying Kohonen's self-organizing feature maps (SOFMs) are applied to the adaptive control of radio resources to minimize interference, hence, maximize capacity in direct-sequence (DS) CDMA networks. The approach based on SOFMs is applied to some published examples of both theoretical and empirical models of DS/CDMA microcellular networks in metropolitan areas. The results of the approach for these examples are informally compared to the performance of algorithms, based on Hopfield- Tank neural networks and on genetic algorithms, for the channel assignment problem.

76 FR 77550 - Extension of Approved Information Collection; OMB Control No. 1004-0162

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-13

...'' regardless of the form of your comments. FOR FURTHER INFORMATION CONTACT: Barbara Gamble, Division of Fluid... Ms. Gamble. SUPPLEMENTARY INFORMATION: OMB regulations at 5 CFR 1320, which implement provisions of...

Selection, Interviews, and Appointments | Cancer Prevention Fellowship Program

Cancer.gov

The CPFP Scientific Education Committee reviews complete applications submitted on time. This committee is comprised of scientists from different divisions of NCI, and FDA, and a non-NCI expert in cancer prevention and control.

MOBILE EMISSIONS CHARACTERIZATION TEAM (HANDOUT)

EPA Science Inventory

The handout describes the Mobile Emissions Characterization Team of EPA's Office of Research and Development, National Risk Management Research Laboratory, Air Pollution Prevention and Control Division. The team conducts research to characterize and evaluate emissions of volatile...

Regional variation in drug purchase opportunity among youths in the United States, 1996-1997.

PubMed

James, Kirk E; Wagner, Fernando A; Anthony, James C

2002-03-01

This study was designed to examine geographic variation in illegal drug purchase opportunity among young people living in the United States; there was a subfocus on age, sex, and urban/rural residence. Data from the 1996-1997 National Household Surveys on Drug Abuse were analyzed; the nationally representative sample of community residents included 21,531 participants aged 12-24 years old. Respondents were asked if someone had approached them to sell them an illegal drug during the past 30 days. To protect respondents' confidentiality, there is no finegrained geographical coding of data in the National Household Surveys on Drug Abuse public use data files, but nine geographical divisional indicators are provided (i.e., West North Central, New England, etc.). Results indicated males were an estimated 1.8 times more likely than females to have had a recent illicit drug purchase opportunity, and urban residents were 1.5 times more likely than rural residents to have had a recent drug purchase opportunity. As for geographic divisions, the Pacific division surpassed all other divisions: Its residents were 1.5 times more likely to have recent drug purchase opportunities than the West North Central division (used here as a reference category). After controlling statistically for age, sex, and urban/rural residence, residence in four divisions was found to be associated with greater likelihood of an illicit drug purchase opportunity. The observed patterns of drug purchase opportunity add new features to our understanding of illicit drug involvement across the United States.

Dynamics and control of sister kinetochore behavior during the meiotic divisions in Drosophila spermatocytes

PubMed Central

2018-01-01

Sister kinetochores are connected to the same spindle pole during meiosis I and to opposite poles during meiosis II. The molecular mechanisms controlling the distinct behavior of sister kinetochores during the two meiotic divisions are poorly understood. To study kinetochore behavior during meiosis, we have optimized time lapse imaging with Drosophila spermatocytes, enabling kinetochore tracking with high temporal and spatial resolution through both meiotic divisions. The correct bipolar orientation of chromosomes within the spindle proceeds rapidly during both divisions. Stable bi-orientation of the last chromosome is achieved within ten minutes after the onset of kinetochore-microtubule interactions. Our analyses of mnm and tef mutants, where univalents instead of bivalents are present during meiosis I, indicate that the high efficiency of normal bi-orientation depends on pronounced stabilization of kinetochore attachments to spindle microtubules by the mechanical tension generated by spindle forces upon bi-orientation. Except for occasional brief separation episodes, sister kinetochores are so closely associated that they cannot be resolved individually by light microscopy during meiosis I, interkinesis and at the start of meiosis II. Permanent evident separation of sister kinetochores during M II depends on spindle forces resulting from bi-orientation. In mnm and tef mutants, sister kinetochore separation can be observed already during meiosis I in bi-oriented univalents. Interestingly, however, this sister kinetochore separation is delayed until the metaphase to anaphase transition and depends on the Fzy/Cdc20 activator of the anaphase-promoting complex/cyclosome. We propose that univalent bi-orientation in mnm and tef mutants exposes a release of sister kinetochore conjunction that occurs also during normal meiosis I in preparation for bi-orientation of dyads during meiosis II. PMID:29734336

Variability in the mechanisms controlling Southern Ocean phytoplankton bloom phenology in an ocean model and satellite observations

NASA Astrophysics Data System (ADS)

Rohr, Tyler; Long, Matthew C.; Kavanaugh, Maria T.; Lindsay, Keith; Doney, Scott C.

2017-05-01

A coupled global numerical simulation (conducted with the Community Earth System Model) is used in conjunction with satellite remote sensing observations to examine the role of top-down (grazing pressure) and bottom-up (light, nutrients) controls on marine phytoplankton bloom dynamics in the Southern Ocean. Phytoplankton seasonal phenology is evaluated in the context of the recently proposed "disturbance-recovery" hypothesis relative to more traditional, exclusively "bottom-up" frameworks. All blooms occur when phytoplankton division rates exceed loss rates to permit sustained net population growth; however, the nature of this decoupling period varies regionally in Community Earth System Model. Regional case studies illustrate how unique pathways allow blooms to emerge despite very poor division rates or very strong grazing rates. In the Subantarctic, southeast Pacific small spring blooms initiate early cooccurring with deep mixing and low division rates, consistent with the disturbance-recovery hypothesis. Similar systematics are present in the Subantarctic, southwest Atlantic during the spring but are eclipsed by a subsequent, larger summer bloom that is coincident with shallow mixing and the annual maximum in division rates, consistent with a bottom-up, light limited framework. In the model simulation, increased iron stress prevents a similar summer bloom in the southeast Pacific. In the simulated Antarctic zone (70°S-65°S) seasonal sea ice acts as a dominant phytoplankton-zooplankton decoupling agent, triggering a delayed but substantial bloom as ice recedes. Satellite ocean color remote sensing and ocean physical reanalysis products do not precisely match model-predicted phenology, but observed patterns do indicate regional variability in mechanism across the Atlantic and Pacific.

Establishment of effective control factors to achieve federal enforcement consistency with the Highway Beautification Act : a final report submitted to Florida Department of Transportation, Right of Way Division.

DOT National Transportation Integrated Search

2017-10-01

The Highway Beautification Act, which was enforced in 1965, aims to establish an efficient outdoor advertising control (OAC) program for erection and maintenance of the outdoor advertising signs, displays and devices, which are located in a close pro...

Southwestern Division 2012 History Report

DTIC Science & Technology

2013-05-01

maintenance to be performed. Examples of additional maintenance included hydraulic piping replacement, motor control center upgrades, miter gates anchorage...in CEBIS. WATER MANAGEMENT PROGRAM 2012 Hydrology , Hydraulics and Water Management Programs 2012: In March 2012, the Corps’ SWD office...hosted the annual “2012 Reservoir Control Center/Hydrology and Hydraulics annual meeting. The meeting was held in Tulsa, Oklahoma at the Tulsa

78 FR 60880 - Statement of Organization, Functions, and Delegations of Authority

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-02

... Commissioner (KFB) Division of Business and Resource Management (KFB2) Division of Customer Communications... Systems, Division of Business and Resource Management, Division of Customer Communications, Division of...: The Division of Management Services to the Division of Business and Resource Management; the Division...

Publications of the NASA Controlled Ecological Life Support Systems (CELSS) Program 1984-86

NASA Technical Reports Server (NTRS)

1987-01-01

Publications of research sponsored by the NASA CELSS (Controlled Ecological Life Support Systems) Program are listed, along with publications of interest to the Program. The bibliography is divided into the three major divisions of CELSS research: (1) Food Production; (2) Waste Management; and (3) Systems Management and Control. This bibliography is an update of NASA CR-3911 and includes references from 1984 through 1986.

Need for Robust Sensors for Inherently Fail-Safe Gas Turbine Engine Controls, Monitoring, and Prognostics (Postprint)

DTIC Science & Technology

2006-09-01

MONITORING , AND PROGNOSTICS Alireza R. Behbahani Controls / Engine Health Management Turbine Engine Division / PRTS U.S. Air Force Research...Technical Report 2005. 8. Greitzer, Frank et al, “Gas Turbine Engine Health Monitoring and Prognostics ”, International Society of Logistics (SOLE...AFRL-PR-WP-TP-2007-217 NEED FOR ROBUST SENSORS FOR INHERENTLY FAIL-SAFE GAS TURBINE ENGINE CONTROLS, MONITORING , AND PROGNOSTICS (POSTPRINT

Cross Polarization Interference Reduction Techniques

DTIC Science & Technology

1979-06-01

Description of the Conceptual Design ................... 2-3 2.1.2.1 Performance Measurement Circuitry ............ . ....... 2-6 2.1.2.2 Control Logic...for publication. APPROVED: FRE"DERIC1K D. SCWTANDT Prqjec1 Engineer APPROVED: Technical Director Comunicatiorna and Control Division Th2R TUHE C...Griffiss AFB NY 13441 UBHOPAE 14. MONITORING AGENCY NArJE & ADDRESS(II diferent from Controling office) IS. SECUR!TY CLASS. (.1 this roport) Same

Handling Quality Requirements for Advanced Aircraft Design: Longitudinal Mode

DTIC Science & Technology

1979-08-01

phases of air -to- air combat, for example). This is far simpler than the general problem of control law definition. How- ever, the results of such...unlimited. Ali FORCE FUGHT DYNAMICS LABORATORYAIR FORCE WRIGHT AERONAUTICALLABORATORIES AIR FORCE SYSTEMS COMMANDI * WRIGHT-PATITERSON AIR FORCE BASE...not necessarily shared by the Air Force. Brian. W. VauVliet Project Engineer S Rorad0. Anderson, Chief Control Dynamics Branch Flight Control Division

16 CFR 1500.43a - Method of test for flashpoint of volatile flammable materials.

Code of Federal Regulations, 2012 CFR

2012-01-01

... between the temperature control dial and the thermometer readings at each major (numbered) dial division... block of suitable conductivity with a cylindrical depression, or sample cup, over which is fitted a...

16 CFR 1500.43a - Method of test for flashpoint of volatile flammable materials.

Code of Federal Regulations, 2011 CFR

2011-01-01

... between the temperature control dial and the thermometer readings at each major (numbered) dial division... block of suitable conductivity with a cylindrical depression, or sample cup, over which is fitted a...

16 CFR 1500.43a - Method of test for flashpoint of volatile flammable materials.

Code of Federal Regulations, 2014 CFR

2014-01-01

... between the temperature control dial and the thermometer readings at each major (numbered) dial division... block of suitable conductivity with a cylindrical depression, or sample cup, over which is fitted a...

Litter survey : status report 1.

DOT National Transportation Integrated Search

1979-01-01

At the request of the Division of Litter Control of the Department of Conservation and Economic Development, the Research Council has initiated a litter survey program at approximately 25 sites on the state highway system. The purpose of this program...

75 FR 5624 - Extension of Approval of Information Collection, OMB Control Number 1004-0137

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-03

... information collection request, contact Barbara Gamble, Division of Fluid Minerals, at 202-912-7148... Information Relay Service (FIRS) at 1-800-877-8339, to leave a message for Ms. Gamble. SUPPLEMENTARY...

40 CFR 52.1126 - Control strategy: Sulfur oxides.

Code of Federal Regulations, 2012 CFR

2012-07-01

... granted by the Massachusetts Department of Environmental Quality Engineering.) Massachusetts Mutual Life... stack). Central Massachusetts APCD Borden, Inc., Chemical Division, Leominster (conditioned upon first... Department of Environmental Quality Engineering.). Gardner State Hospital, Gardner. Grafton State Hospital...

78 FR 8591 - Notice of Determinations Regarding Eligibility To Apply for Worker Adjustment Assistance

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-06

... Dura Automotive Systems Milan, TN December 6, 2011. Cable Operations LLC, Control Systems Div... Corporate Division. 82,304 Tyco Electronics, Telecom Shakopee, MN March 3, 2013. Networks Business Unit. 82...

40 CFR 52.1126 - Control strategy: Sulfur oxides.

Code of Federal Regulations, 2010 CFR

2010-07-01

... granted by the Massachusetts Department of Environmental Quality Engineering.) Massachusetts Mutual Life... stack). Central Massachusetts APCD Borden, Inc., Chemical Division, Leominster (conditioned upon first... Department of Environmental Quality Engineering.). Gardner State Hospital, Gardner. Grafton State Hospital...

40 CFR 52.1126 - Control strategy: Sulfur oxides.

Code of Federal Regulations, 2011 CFR

2011-07-01

... granted by the Massachusetts Department of Environmental Quality Engineering.) Massachusetts Mutual Life... stack). Central Massachusetts APCD Borden, Inc., Chemical Division, Leominster (conditioned upon first... Department of Environmental Quality Engineering.). Gardner State Hospital, Gardner. Grafton State Hospital...

75 FR 38128 - Sensata Technologies MA, Inc., Power Controls Division, Formerly Known As Airpax Corp., Cambridge...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-01

... subject firm. The workers are engaged in activities related to the production of hydraulic magnetic... shift in production of hydraulic magnetic circuit breakers to Aguascalientes, Mexico. The amended notice...

Optimality in the Development of Intestinal Crypts

PubMed Central

Itzkovitz, Shalev; Blat, Irene C.; Jacks, Tyler; Clevers, Hans; van Oudenaarden, Alexander

2012-01-01

SUMMARY Intestinal crypts in mammals are comprised of long-lived stem cells and shorter-lived progenies. These two populations are maintained in specific proportions during adult life. Here, we investigate the design principles governing the dynamics of these proportions during crypt morphogenesis. Using optimal control theory, we show that a proliferation strategy known as a “bang-bang” control minimizes the time to obtain a mature crypt. This strategy consists of a surge of symmetric stem cell divisions, establishing the entire stem cell pool first, followed by a sharp transition to strictly asymmetric stem cell divisions, producing nonstem cells with a delay. We validate these predictions using lineage tracing and single-molecule fluorescence in situ hybridization of intestinal crypts in infant mice, uncovering small crypts that are entirely composed of Lgr5-labeled stem cells, which become a minority as crypts continue to grow. Our approach can be used to uncover similar design principles in other developmental systems. PMID:22304925

Optimality in the Development of Intestinal Crypts

NASA Astrophysics Data System (ADS)

van Oudenaarden, Alexander

2012-02-01

Intestinal crypts in mammals are comprised of long-lived stem cells and shorter-lived progenies, maintained under tight proportions during adult life. Here we ask what are the design principles that govern the dynamics of these proportions during crypt morphogenesis. We use optimal control theory to show that a stem cell proliferation strategy known as a `bang-bang' control minimizes the time to obtain a mature crypt. This strategy consists of a surge of symmetric stem cell divisions, establishing the entire stem cell pool first, followed by a sharp transition to strictly asymmetric stem cell divisions, producing non-stem cells with a delay. We validate these predictions using lineage tracing and single molecule fluorescent in-situ hybridization of intestinal crypts in newborn mice and find that small crypts are entirely composed of Lgr5 stem cells, which become a minority as crypts further grow. Our approach can be used to uncover similar design principles in other developmental systems.

Cell division versus cell elongation: the control of radicle elongation during thermoinhibition of Tagetes minuta achenes.

PubMed

Taylor, Nicky J; Hills, Paul N; van Staden, Johannes

2007-12-01

Endogenous embryo factors, which act mainly in the radicle, prevent germination in Tagetes minuta at high temperatures. These factors act to prevent cell elongation, which is critical for radicle protrusion under optimal conditions. Once the radicle has emerged both cell elongation and cell division are required for post-germination growth. Germination can be induced at high temperatures by fusicoccin, which rapidly stimulates cell elongation. In addition, priming seeds at 25 degrees C on polyethylene glycol (PEG) 6000 and mannitol could also induce germination on water at 36 degrees C, indicating that priming prevents radicle protrusion at a point subsequent to the point of control in thermoinhibited achenes. Flow cytometry studies revealed that DNA synthesis occurs during thermoinhibition and the inhibition of DNA synthesis during this process inhibits subsequent germination on water under optimal conditions, suggesting a protective role for DNA synthesis in thermoinhibited achenes of T. minuta.

Ready, Willing, and Able? Impediments to the Onset of Marital Fertility Decline in the United States

PubMed Central

Hacker, J. David

2016-01-01

This study relies on IPUMS samples of the 1850, 1860, 1870, and 1880 censuses, aggregate census data, and the timing of state laws criminalizing abortion to construct regional estimates of marital fertility in the United States and estimate correlates of marital fertility. The results show a significant lag between the onset of marital fertility decline in the nation’s northeastern census divisions and its onset in western and southern census divisions. Empirical models indicate the presence of cultural, economic, and legal impediments to the diffusion of marital fertility control and illustrate the need for more inclusive models of fertility decline. PMID:27757800

Veterinary public health activities at FAO: echinococcosis/hydatid disease.

PubMed

Eddi, C; de Balogh, K; Lubroth, J; Amanfu, W; Speedy, A; Battaglia, D

2004-12-01

Cystic hydatidosis is a zoonotic disease that remain as a significant cause of human morbidity and mortality in many parts of the world. The disease has veterinary public health implications. FAO is involved with some activities in the control of echinococcosis/hydatid disease: within the Animal Production and Health Division the Veterinary Public Health (VHP) Programme is constituted by members of the different Services (Animal Health, Animal Production, and Livestock Policy) within the Division. FAO regular programme has also established a global network of professionals directly involved in VPH. Furthermore FAO's Technical Cooperation Projects (TCP) is a tool to assist member countries in responding to urgent and unforeseen demands.

Ready, Willing, and Able? Impediments to the Onset of Marital Fertility Decline in the United States.

PubMed

Hacker, J David

2016-12-01

This study relies on IPUMS samples of the 1850, 1860, 1870, and 1880 censuses, aggregate census data, and the timing of state laws criminalizing abortion to construct regional estimates of marital fertility in the United States and estimate correlates of marital fertility. The results show a significant lag between the onset of marital fertility decline in the nation's northeastern census divisions and its onset in western and southern census divisions. Empirical models indicate the presence of cultural, economic, and legal impediments to the diffusion of marital fertility control and illustrate the need for more inclusive models of fertility decline.

Body Size Changes Among National Collegiate Athletic Association New England Division III Football Players, 1956-2014: Comparison With Age-Matched Population Controls.

PubMed

Elliott, Kayla R; Harmatz, Jerold S; Zhao, Yanli; Greenblatt, David J

2016-05-01

Collegiate football programs encourage athletes to pursue high body weights. To examine position-dependent trends over time in body size characteristics among football players in the National Collegiate Athletic Association Division III New England Small College Athletic Conference (NESCAC) from 1956 to 2014 and to compare the observed absolute and relative changes with those in age-matched male population controls. Descriptive laboratory study. Medical school affiliated with a NESCAC institution. Football team rosters from the 10-member NESCAC schools, available as public documents, were analyzed along with body size data from general population males aged 20 to 29 years from the National Health and Nutrition Examination Survey (NHANES). Body weight, height, and calculated body mass index were evaluated using analysis of variance, linear regression, and nonlinear regression to determine the distribution features of size variables and changes associated with time (year), school, and position. Among NESCAC linemen, absolute and relative changes over time in body weight and body mass index exceeded corresponding changes in the NHANES population controls. New England Small College Athletic Conference offensive linemen body weights increased by 37.5% from 1956 to 2014 (192 to 264 lb [86.4 to 118.8 kg]), compared with a 12% increase (164 to 184 lb [73.8 to 82.8 kg]) since 1961 in the NHANES population controls. Body mass index changed in parallel with body weight and exceeded 35 kg/m(2) in more than 30% of contemporary NESCAC offensive linemen. Among skill players in the NESCAC group, time-related changes in body size characteristics generally paralleled those in the NHANES controls. High body weight and body mass indices were evident in offensive linemen, even among those in Division III football programs with no athletic scholarships. These characteristics may be associated with adverse cardiovascular and metabolic outcomes. We need approaches to encourage risk modification in the postfootball lifestyles of these individuals.

How oocytes try to get it right: spindle checkpoint control in meiosis.

PubMed

Touati, Sandra A; Wassmann, Katja

2016-06-01

The generation of a viable, diploid organism depends on the formation of haploid gametes, oocytes, and spermatocytes, with the correct number of chromosomes. Halving the genome requires the execution of two consecutive specialized cell divisions named meiosis I and II. Unfortunately, and in contrast to male meiosis, chromosome segregation in oocytes is error prone, with human oocytes being extraordinarily "meiotically challenged". Aneuploid oocytes, that are with the wrong number of chromosomes, give rise to aneuploid embryos when fertilized. In humans, most aneuploidies are lethal and result in spontaneous abortions. However, some trisomies survive to birth or even adulthood, such as the well-known trisomy 21, which gives rise to Down syndrome (Nagaoka et al. in Nat Rev Genet 13:493-504, 2012). A staggering 20-25 % of oocytes ready to be fertilized are aneuploid in humans. If this were not bad enough, there is an additional increase in meiotic missegregations as women get closer to menopause. A woman above 40 has a risk of more than 30 % of getting pregnant with a trisomic child. Worse still, in industrialized western societies, child birth is delayed, with women getting their first child later in life than ever. This trend has led to an increase of trisomic pregnancies by 70 % in the last 30 years (Nagaoka et al. in Nat Rev Genet 13:493-504, 2012; Schmidt et al. in Hum Reprod Update 18:29-43, 2012). To understand why errors occur so frequently during the meiotic divisions in oocytes, we review here the molecular mechanisms at works to control chromosome segregation during meiosis. An important mitotic control mechanism, namely the spindle assembly checkpoint or SAC, has been adapted to the special requirements of the meiotic divisions, and this review will focus on our current knowledge of SAC control in mammalian oocytes. Knowledge on how chromosome segregation is controlled in mammalian oocytes may help to identify risk factors important for questions related to human reproductive health.

Laboratory directed research and development program FY 1997

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1998-03-01

This report compiles the annual reports of Laboratory Directed Research and Development projects supported by the Berkeley Lab. Projects are arranged under the following topical sections: (1) Accelerator and fusion research division; (2) Chemical sciences division; (3) Computing Sciences; (4) Earth sciences division; (5) Environmental energy technologies division; (6) life sciences division; (7) Materials sciences division; (8) Nuclear science division; (9) Physics division; (10) Structural biology division; and (11) Cross-divisional. A total of 66 projects are summarized.

49 CFR 177.841 - Division 6.1 and Division 2.3 materials.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 2 2010-10-01 2010-10-01 false Division 6.1 and Division 2.3 materials. 177.841... PUBLIC HIGHWAY Loading and Unloading § 177.841 Division 6.1 and Division 2.3 materials. (See also § 177...) or Division 6.1 (poisonous) materials. The transportation of a Division 2.3 (poisonous gas) or...

Myosin‑II heavy chain and formin mediate the targeting of myosin essential light chain to the division site before and during cytokinesis

PubMed Central

Feng, Zhonghui; Okada, Satoshi; Cai, Guoping; Zhou, Bing; Bi, Erfei

2015-01-01

MLC1 is a haploinsufficient gene encoding the essential light chain for Myo1, the sole myosin‑II heavy chain in the budding yeast Saccharomyces cerevisiae. Mlc1 defines an essential hub that coordinates actomyosin ring function, membrane trafficking, and septum formation during cytokinesis by binding to IQGAP, myosin‑II, and myosin‑V. However, the mechanism of how Mlc1 is targeted to the division site during the cell cycle remains unsolved. By constructing a GFP‑tagged MLC1 under its own promoter control and using quantitative live‑cell imaging coupled with yeast mutants, we found that septin ring and actin filaments mediate the targeting of Mlc1 to the division site before and during cytokinesis, respectively. Both mechanisms contribute to and are collectively required for the accumulation of Mlc1 at the division site during cytokinesis. We also found that Myo1 plays a major role in the septin‑dependent Mlc1 localization before cytokinesis, whereas the formin Bni1 plays a major role in the actin filament–dependent Mlc1 localization during cytokinesis. Such a two‑tiered mechanism for Mlc1 localization is presumably required for the ordered assembly and robustness of cytokinesis machinery and is likely conserved across species. PMID:25631819

Antiproliferative effects of yogurt fractions obtained by membrane dialysis on cultured mammalian intestinal cells.

PubMed

Ganjam, L S; Thornton, W H; Marshall, R T; MacDonald, R S

1997-10-01

The consumption of yogurt has been associated with a reduced incidence of colon cancer in population groups. Bioactive peptides produced during bacterial fermentation may alter the risk of colon cancer via modification of cell proliferation in the colon. Using our previously described cell culture model system, we have isolated a yogurt fraction that decreases cell proliferation. Yogurt was fractionated using 10,000- and 500-Da membrane dialysis. When the yogurt fraction was incubated with IEC-6 or Caco-2 cells, cell division was decreased compared with control treatments, as determined by thymidine incorporation. Cell division was not inhibited in response to a similarly produced milk fraction or in response to solutions of lactic acid. The determination of cell kinetics by flow cytometry revealed a decrease in the number of cells in the initial growth phase in response to the yogurt fraction for the IEC-6 cells, but not the Caco-2 cells. Alpha-Lactalbumin inhibited cell division of both cell lines, but beta-casein did not.

Regulation of the expression of the cell-cycle gene ftsZ by DicF antisense RNA. Division does not require a fixed number of FtsZ molecules.

PubMed

Tétart, F; Bouché, J P

1992-03-01

We show that the 53-nucleotide RNA molecule encoded by gene dicF blocks cell division in Escherichia coli by inhibiting the translation of ftsZ mRNA. Such a role for dicF had been predicted on the basis of the complementarity of DicF RNA with the ribosome-binding region of the ftsZ mRNA. An analysis of ftsZ expression at its chromosomal locus, and of an ftsZ-lacZ translational fusion controlled by promoters ftsZ1p and ftsZ2p only, indicates that ftsZ is not autoregulated. Partial inhibition of FtsZ synthesis leads to increased cell size. However, the number of FtsZ molecules per cell can be reduced threefold without affecting the division rate significantly. Our results suggest that septation is not triggered by a fixed number of newly synthesized FtsZ molecules per cell.

The Biochemistry of Mitosis

PubMed Central

Wieser, Samuel; Pines, Jonathon

2015-01-01

In this article, we will discuss the biochemistry of mitosis in eukaryotic cells. We will focus on conserved principles that, importantly, are adapted to the biology of the organism. It is vital to bear in mind that the structural requirements for division in a rapidly dividing syncytial Drosophila embryo, for example, are markedly different from those in a unicellular yeast cell. Nevertheless, division in both systems is driven by conserved modules of antagonistic protein kinases and phosphatases, underpinned by ubiquitin-mediated proteolysis, which create molecular switches to drive each stage of division forward. These conserved control modules combine with the self-organizing properties of the subcellular architecture to meet the specific needs of the cell. Our discussion will draw on discoveries in several model systems that have been important in the long history of research on mitosis, and we will try to point out those principles that appear to apply to all cells, compared with those in which the biochemistry has been specifically adapted in a particular organism. PMID:25663668

PASTA repeats of the protein kinase StkP interconnect cell constriction and separation of Streptococcus pneumoniae.

PubMed

Zucchini, Laure; Mercy, Chryslène; Garcia, Pierre Simon; Cluzel, Caroline; Gueguen-Chaignon, Virginie; Galisson, Frédéric; Freton, Céline; Guiral, Sébastien; Brochier-Armanet, Céline; Gouet, Patrice; Grangeasse, Christophe

2018-02-01

Eukaryotic-like serine/threonine kinases (eSTKs) with extracellular PASTA repeats are key membrane regulators of bacterial cell division. How PASTA repeats govern eSTK activation and function remains elusive. Using evolution- and structural-guided approaches combined with cell imaging, we disentangle the role of each PASTA repeat of the eSTK StkP from Streptococcus pneumoniae. While the three membrane-proximal PASTA repeats behave as interchangeable modules required for the activation of StkP independently of cell wall binding, they also control the septal cell wall thickness. In contrast, the fourth and membrane-distal PASTA repeat directs StkP localization at the division septum and encompasses a specific motif that is critical for final cell separation through interaction with the cell wall hydrolase LytB. We propose a model in which the extracellular four-PASTA domain of StkP plays a dual function in interconnecting the phosphorylation of StkP endogenous targets along with septal cell wall remodelling to allow cell division of the pneumococcus.

LGN plays distinct roles in oral epithelial stratification, filiform papilla morphogenesis and hair follicle development

PubMed Central

Lough, Kendall J.; Patel, Jeet H.; Descovich, Carlos Patiño; Curtis, T. Anthony

2016-01-01

Oral epithelia protect against constant challenges by bacteria, viruses, toxins and injury while also contributing to the formation of ectodermal appendages such as teeth, salivary glands and lingual papillae. Despite increasing evidence that differentiation pathway genes are frequently mutated in oral cancers, comparatively little is known about the mechanisms that regulate normal oral epithelial development. Here, we characterize oral epithelial stratification and describe multiple distinct functions for the mitotic spindle orientation gene LGN (Gpsm2) in promoting differentiation and tissue patterning in the mouse oral cavity. Similar to its function in epidermis, apically localized LGN directs perpendicular divisions that promote stratification of the palatal, buccogingival and ventral tongue epithelia. Surprisingly, however, in dorsal tongue LGN is predominantly localized basally, circumferentially or bilaterally and promotes planar divisions. Loss of LGN disrupts the organization and morphogenesis of filiform papillae but appears to be dispensable for embryonic hair follicle development. Thus, LGN has crucial tissue-specific functions in patterning surface ectoderm and its appendages by controlling division orientation. PMID:27317810

Introduction to Robust Multivariable Control

DTIC Science & Technology

1986-02-01

AX DAVIS, Assistant for Research and Technology Flight Control Division Flight Dynamics Laboratory "If your address has changed, if you wish to be...particular chapter is given at the end of each chapter. It is needless to say that there are numerour other - publications in this area of controls research ...tco in this Area of research comprehensively in a single report. Finally, even though most of the material in this report comes from publications of W

Computer Decision Support Software Safely Improves Glycemic Control in the Burn Intensive Care Unit: A Randomized Controlled Clinical Study

DTIC Science & Technology

2011-01-01

Program Jointly Managed by the USA MRMC, NIH, NASA, and the Juvenile Diabetes Research Foundation and Combat Casualty Care Division, United States Army...were performed in the CP group (p = 0.0003), and nursing staff compliance with CP recommendations was greater (p < 0.0001). Conclusions—Glycemic...enhanced consistency in practice, providing standardization among nursing staff. Keywords Glycemic control; hypoglycemia; computer decision support

Heavy Oil Recovery Ohmsett Test Report

DTIC Science & Technology

2012-06-01

systems’ effects on wildlife and the bottom environment is also needed. Use of sonar or laser may be limited by the presence of marine mammals or other...Technology Corporation, Marine Pollution Control, and the Oil Stop Division of American Pollution Control. In 2011, these three companies were awarded...ROVs may need more power and the pump intake nozzle may need to be smaller.  Marine Pollution Control (MPC) designed a system based on a manned

Views of the mission control center during STS-9

NASA Technical Reports Server (NTRS)

1983-01-01

Busy moment in the customer management room (CMR) of JSC's mission control center during Spacelab 1 day 2. Three personnel from the European Space Agency (ESA) huddle around a console along with Ralph Hoodless (seated at left), of the George C. Marshall Space Flight Center. Others pictured are Lars Tedeman and Hildegard Binck (standing); and Frank Longhurst (seated right). Tedeman is with ESA's quality control division and Longhurst is Spacelab operations manager.

’Do-It-Yourself’ Fallout/Blast Shelter Evaluation

DTIC Science & Technology

1984-03-01

N4AME & AOORIESS(I! dittvrevI !M’", Controlling Olif~t) IS. SEC’.JRITY CL-ASS. (GO this report) Lawrence Livermore National Laboratory Unclassified P...the data from the transient recorder iemory tirough the Computer Automated Measurement and Control (CAMAC) data busa und stores them on an $-inch...Command and Control Technical Center Emergency Technology Division Department of Defense 0a& Ridge Natioual Laboratory The Pentagon Attn: Librarian

IFLA General Conference, 1987. Division on Bibliographic Control. Bibliography and Round Table on A/V Media Section. Papers.

ERIC Educational Resources Information Center

International Federation of Library Associations, The Hague (Netherlands).

The two papers in this section focus on the bibliographic control of sound recordings, primarily phonograph records. In the first, "The National Discography for the United Kingdom," Christopher Roads discusses the problem of lack of easily accessible and up-to-date information about the growing collection of new sound recordings as they…

Kinetics and clonality of immunological memory in humans.

PubMed

Beverley, Peter C L

2004-10-01

T-cell immunological memory consists largely of clones of proliferating lymphocytes maintained by antigenic stimulation and the survival and proliferative effects of cytokines. The duration of survival of memory clones in humans is determine by the Hayflick limit on the number of cell divisions, the rate of cycling of memory cells and factors that control erosion of telomeres, including mechanisms that control telomerase.

Emergence of telerobotic control enhancement from research in machine autonomy

NASA Astrophysics Data System (ADS)

Haddad, Albert G., Sr.; Adams, John C.; Berardo, Peter A.; Ohlund, Kent O.; Van Vactor, David L.

1992-03-01

This paper provides a description of the Robotic Research Program being conducted at the Lockheed Research and Development Division Laboratories. It details the approach taken to fuse autonomy with teleoperative control. The component/enabling technologies are defined and the status of the development of those technologies is reported. CASE tools used in an accelerated development environment are identified and discussed.

Prevalence of Disordered Eating and Pathogenic Weight Control Behaviors among NCAA Division I Female Collegiate Gymnasts and Swimmers

ERIC Educational Resources Information Center

Anderson, Carlin; Petrie, Trent A.

2012-01-01

Eating disorders and related weight control behaviors, such as excessive exercising and restrictive eating, represent serious health problems for girls and women in the United States and other industrialized nations. Female athletes, in particular, have been identified as a subgroup to study because of the unique weight, performance, and body…

Process for control of cell division

NASA Technical Reports Server (NTRS)

Cone, C. D., Jr. (Inventor)

1977-01-01

A method of controlling mitosis of biological cells was developed, which involved inducing a change in the intracellular ionic hierarchy accompanying the cellular electrical transmembrane potential difference (Esubm) of the cells. The ionic hierarchy may be varied by imposing changes on the relative concentrations of Na(+), K(+) and Cl(-), or by directly imposing changes in the physical Esubm level across the cell surface.

Best Manufacturing Practices Survey Conducted at General Dynamics, Fort Worth Division, Fort Worth, Texas

DTIC Science & Technology

1988-05-01

funded by the U.S. Air Force and GDFW. The system will be capable of unmanned operation and will encompass design, engineering , fabrication, and product...Industrial Engineering Production (309) 782-4619 ActivityRock Island, IL Richard Celin Naval Air Engineering Center Production (201) 323-2173 Lakehurst...CONFIGURATION CONTROL Engineering Change Control Room ............................................ 15 Implementatlon af Retofit Changes

16 CFR § 1500.43a - Method of test for flashpoint of volatile flammable materials.

Code of Federal Regulations, 2013 CFR

2013-01-01

... between the temperature control dial and the thermometer readings at each major (numbered) dial division... block of suitable conductivity with a cylindrical depression, or sample cup, over which is fitted a...

40 CFR 205.4 - Inspection and monitoring.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Section 205.4 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS General Provisions § 205.4 Inspection and... comply with these regulations. (b) The Director, Noise Enforcement Division, may request that a...

77 FR 30972 - Certain Polybrominated Diphenylethers; Significant New Use Rule and Test Rule; Extension of...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-24

... technical information contact: Catherine Roman, Chemical Control Division, Office of Pollution Prevention... Safety and Pollution Prevention. [FR Doc. 2012-12625 Filed 5-23-12; 8:45 am] BILLING CODE 6560-50-P ...

STS-3 FLIGHT DAY 1 ACTIVITIES - MISSION OPERATIONS CONTROL ROOM (MOCR) - JSC

NASA Image and Video Library

1982-03-22

MOCR during Flight Day 1 of the STS-3 Mission. View: Thomas L. Moser, of the Structures and Mechanics Division, briefing Flight Director Eugene Kranz, Flight Operations, and Dr. Kraft, JSC Director. JSC, HOUSTON, TX

Engineering Administration.

ERIC Educational Resources Information Center

Naval Personnel Program Support Activity, Washington, DC.

This book is intended to acquaint naval engineering officers with their duties in the engineering department. Standard shipboard organizations are analyzed in connection with personnel assignments, division operations, and watch systems. Detailed descriptions are included for the administration of directives, ship's bills, damage control, training…

75 FR 5623 - Extension of Approval of Information Collection, OMB Control Number 1004-0132

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-03

... Barbara Gamble, Division of Fluid Minerals, at 202-912-7148 (Commercial or FTS). Persons who use a..., to leave a message for Ms. Gamble. SUPPLEMENTARY INFORMATION: OMB regulations at 5 CFR 1320, which...

40 CFR 205.4 - Inspection and monitoring.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Section 205.4 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS General Provisions § 205.4 Inspection and... comply with these regulations. (b) The Director, Noise Enforcement Division, may request that a...

77 FR 28281 - Withdrawal of Revocation of TSCA Section 4 Testing Requirements for One High Production Volume...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-14

... information contact: Catherine Roman, Chemical Control Division (7405M), Office of Pollution Prevention and... number: (202) 564-8157; email address: roman[email protected] . For general information contact: The...

The Arabidopsis arc5 and arc6 mutations differentially affect plastid morphology in pavement and guard cells in the leaf epidermis.

PubMed

Fujiwara, Makoto T; Yasuzawa, Mana; Kojo, Kei H; Niwa, Yasuo; Abe, Tomoko; Yoshida, Shigeo; Nakano, Takeshi; Itoh, Ryuuichi D

2018-01-01

Chloroplasts, or photosynthetic plastids, multiply by binary fission, forming a homogeneous population in plant cells. In Arabidopsis thaliana, the division apparatus (or division ring) of mesophyll chloroplasts includes an inner envelope transmembrane protein ARC6, a cytoplasmic dynamin-related protein ARC5 (DRP5B), and members of the FtsZ1 and FtsZ2 families of proteins, which co-assemble in the stromal mid-plastid division ring (FtsZ ring). FtsZ ring placement is controlled by several proteins, including a stromal factor MinE (AtMinE1). During leaf mesophyll development, ARC6 and AtMinE1 are necessary for FtsZ ring formation and thus plastid division initiation, while ARC5 is essential for a later stage of plastid division. Here, we examined plastid morphology in leaf epidermal pavement cells (PCs) and stomatal guard cells (GCs) in the arc5 and arc6 mutants using stroma-targeted fluorescent proteins. The arc5 PC plastids were generally a bit larger than those of the wild type, but most had normal shapes and were division-competent, unlike mutant mesophyll chloroplasts. The arc6 PC plastids were heterogeneous in size and shape, including the formation of giant and mini-plastids, plastids with highly developed stromules, and grape-like plastid clusters, which varied on a cell-by-cell basis. Moreover, unique plastid phenotypes for stomatal GCs were observed in both mutants. The arc5 GCs rarely lacked chlorophyll-bearing plastids (chloroplasts), while they accumulated minute chlorophyll-less plastids, whereas most GCs developed wild type-like chloroplasts. The arc6 GCs produced large chloroplasts and/or chlorophyll-less plastids, as previously observed, but unexpectedly, their chloroplasts/plastids exhibited marked morphological variations. We quantitatively analyzed plastid morphology and partitioning in paired GCs from wild-type, arc5, arc6, and atminE1 plants. Collectively, our results support the notion that ARC5 is dispensable in the process of equal division of epidermal plastids, and indicate that dysfunctions in ARC5 and ARC6 differentially affect plastid replication among mesophyll cells, PCs, and GCs within a single leaf.

A bHLH-Based Feedback Loop Restricts Vascular Cell Proliferation in Plants.

PubMed

Vera-Sirera, Francisco; De Rybel, Bert; Úrbez, Cristina; Kouklas, Evangelos; Pesquera, Marta; Álvarez-Mahecha, Juan Camilo; Minguet, Eugenio G; Tuominen, Hannele; Carbonell, Juan; Borst, Jan Willem; Weijers, Dolf; Blázquez, Miguel A

2015-11-23

Control of tissue dimensions in multicellular organisms requires the precise quantitative regulation of mitotic activity. In plants, where cells are immobile, tissue size is achieved through control of both cell division orientation and mitotic rate. The bHLH transcription factor heterodimer formed by target of monopteros5 (TMO5) and lonesome highway (LHW) is a central regulator of vascular width-increasing divisions. An important unanswered question is how its activity is limited to specify vascular tissue dimensions. Here we identify a regulatory network that restricts TMO5/LHW activity. We show that thermospermine synthase ACAULIS5 antagonizes TMO5/LHW activity by promoting the accumulation of SAC51-LIKE (SACL) bHLH transcription factors. SACL proteins heterodimerize with LHW-therefore likely competing with TMO5/LHW interactions-prevent activation of TMO5/LHW target genes, and suppress the over-proliferation caused by excess TMO5/LHW activity. These findings connect two thus-far disparate pathways and provide a mechanistic understanding of the quantitative control of vascular tissue growth. Copyright © 2015 Elsevier Inc. All rights reserved.

The Use of Protein Hydrolysates for Weed Control

NASA Astrophysics Data System (ADS)

Christians, Nick; Liu, Dianna; Unruh, Jay Bryan

Corn gluten meal, the protein fraction of corn (Zea mays L.) grain, is commercially used as a natural weed control agent and nitrogen source in horticultural crops and in the turf and ornamental markets. Corn gluten hydrolysate, a water soluble form of gluten meal, has also been proposed for the same purpose, although it could be sprayed on the soil rather than applied in the granular form. Five depeptides, glutaminyl-glutamine (Gln-Gln), glycinyl-alanine (Gly-Ala), alanyl-­glutamine (Ala-Glu), alanyl-asparagine (Ala-Asp), and alaninyl-alanine (Ala-Ala) and a pentapeptide leucine-serine-proline-alanine-glutamine (Leu-Ser-Pro-Ala-Gln) were identified as the active components of the hydrolysate. Microscopic analysis revealed that Ala-Ala acted on some metabolic process rather than directly on the mitotic apparatus. Similar to the chloracetamides and sulfonyl-urea hebicides, Ala-Ala inhibits cell division rather than disrupting of cell division processes. Cellular ultrastructure changes caused by exposure to Ala-Ala implicate Ala-Ala as having membrane-disrupting characteristics similar to several synthetic herbicides. The potential use of the hydrolysate and the peptides as weed controls is discussed.

Cross-regulation between Aurora B and Citron kinase controls midbody architecture in cytokinesis

PubMed Central

McKenzie, Callum; Bassi, Zuni I.; Debski, Janusz; Gottardo, Marco; Callaini, Giuliano; Dadlez, Michal; D'Avino, Pier Paolo

2016-01-01

Cytokinesis culminates in the final separation, or abscission, of the two daughter cells at the end of cell division. Abscission relies on an organelle, the midbody, which forms at the intercellular bridge and is composed of various proteins arranged in a precise stereotypic pattern. The molecular mechanisms controlling midbody organization and function, however, are obscure. Here we show that proper midbody architecture requires cross-regulation between two cell division kinases, Citron kinase (CIT-K) and Aurora B, the kinase component of the chromosomal passenger complex (CPC). CIT-K interacts directly with three CPC components and is required for proper midbody architecture and the orderly arrangement of midbody proteins, including the CPC. In addition, we show that CIT-K promotes Aurora B activity through phosphorylation of the INCENP CPC subunit at the TSS motif. In turn, Aurora B controls CIT-K localization and association with its central spindle partners through phosphorylation of CIT-K's coiled coil domain. Our results identify, for the first time, a cross-regulatory mechanism between two kinases during cytokinesis, which is crucial for establishing the stereotyped organization of midbody proteins. PMID:27009191

A Brief Survey of Media Access Control, Data Link Layer, and Protocol Technologies for Lunar Surface Communications

NASA Technical Reports Server (NTRS)

Wallett, Thomas M.

2009-01-01

This paper surveys and describes some of the existing media access control and data link layer technologies for possible application in lunar surface communications and the advanced wideband Direct Sequence Code Division Multiple Access (DSCDMA) conceptual systems utilizing phased-array technology that will evolve in the next decade. Time Domain Multiple Access (TDMA) and Code Division Multiple Access (CDMA) are standard Media Access Control (MAC) techniques that can be incorporated into lunar surface communications architectures. Another novel hybrid technique that is recently being developed for use with smart antenna technology combines the advantages of CDMA with those of TDMA. The relatively new and sundry wireless LAN data link layer protocols that are continually under development offer distinct advantages for lunar surface applications over the legacy protocols which are not wireless. Also several communication transport and routing protocols can be chosen with characteristics commensurate with smart antenna systems to provide spacecraft communications for links exhibiting high capacity on the surface of the Moon. The proper choices depend on the specific communication requirements.

Ovarian Control of Nectar Collection in the Honey Bee (Apis mellifera)

PubMed Central

Siegel, Adam J.; Freedman, Colin; Page, Robert E.

2012-01-01

Honey bees are a model system for the study of division of labor. Worker bees demonstrate a foraging division of labor (DOL) by biasing collection towards carbohydrates (nectar) or protein (pollen). The Reproductive ground-plan hypothesis of Amdam et al. proposes that foraging DOL is regulated by the networks that controlled foraging behavior during the reproductive life cycle of honey bee ancestors. Here we test a proposed mechanism through which the ovary of the facultatively sterile worker impacts foraging bias. The proposed mechanism suggests that the ovary has a regulatory effect on sucrose sensitivity, and sucrose sensitivity impacts nectar loading. We tested this mechanism by measuring worker ovary size (ovariole number), sucrose sensitivity, and sucrose solution load size collected from a rate-controlled artificial feeder. We found a significant interaction between ovariole number and sucrose sensitivity on sucrose solution load size when using low concentration nectar. This supports our proposed mechanism. As nectar and pollen loading are not independent, a mechanism impacting nectar load size would also impact pollen load size. PMID:22558073

Two dynamin-like proteins stabilize FtsZ rings during Streptomyces sporulation.

PubMed

Schlimpert, Susan; Wasserstrom, Sebastian; Chandra, Govind; Bibb, Maureen J; Findlay, Kim C; Flärdh, Klas; Buttner, Mark J

2017-07-25

During sporulation, the filamentous bacteria Streptomyces undergo a massive cell division event in which the synthesis of ladders of sporulation septa convert multigenomic hyphae into chains of unigenomic spores. This process requires cytokinetic Z-rings formed by the bacterial tubulin homolog FtsZ, and the stabilization of the newly formed Z-rings is crucial for completion of septum synthesis. Here we show that two dynamin-like proteins, DynA and DynB, play critical roles in this process. Dynamins are a family of large, multidomain GTPases involved in key cellular processes in eukaryotes, including vesicle trafficking and organelle division. Many bacterial genomes encode dynamin-like proteins, but the biological function of these proteins has remained largely enigmatic. Using a cell biological approach, we show that the two Streptomyces dynamins specifically localize to sporulation septa in an FtsZ-dependent manner. Moreover, dynamin mutants have a cell division defect due to the decreased stability of sporulation-specific Z-rings, as demonstrated by kymographs derived from time-lapse images of FtsZ ladder formation. This defect causes the premature disassembly of individual Z-rings, leading to the frequent abortion of septum synthesis, which in turn results in the production of long spore-like compartments with multiple chromosomes. Two-hybrid analysis revealed that the dynamins are part of the cell division machinery and that they mediate their effects on Z-ring stability during developmentally controlled cell division via a network of protein-protein interactions involving DynA, DynB, FtsZ, SepF, SepF2, and the FtsZ-positioning protein SsgB.

Evaluating Effects of Divided Hemispheric Processing on Word Recognition in Foveal and Extrafoveal Displays: The Evidence from Arabic

PubMed Central

Almabruk, Abubaker A. A.; Paterson, Kevin B.; McGowan, Victoria; Jordan, Timothy R.

2011-01-01

Background Previous studies have claimed that a precise split at the vertical midline of each fovea causes all words to the left and right of fixation to project to the opposite, contralateral hemisphere, and this division in hemispheric processing has considerable consequences for foveal word recognition. However, research in this area is dominated by the use of stimuli from Latinate languages, which may induce specific effects on performance. Consequently, we report two experiments using stimuli from a fundamentally different, non-Latinate language (Arabic) that offers an alternative way of revealing effects of split-foveal processing, if they exist. Methods and Findings Words (and pseudowords) were presented to the left or right of fixation, either close to fixation and entirely within foveal vision, or further from fixation and entirely within extrafoveal vision. Fixation location and stimulus presentations were carefully controlled using an eye-tracker linked to a fixation-contingent display. To assess word recognition, Experiment 1 used the Reicher-Wheeler task and Experiment 2 used the lexical decision task. Results Performance in both experiments indicated a functional division in hemispheric processing for words in extrafoveal locations (in recognition accuracy in Experiment 1 and in reaction times and error rates in Experiment 2) but no such division for words in foveal locations. Conclusions These findings from a non-Latinate language provide new evidence that although a functional division in hemispheric processing exists for word recognition outside the fovea, this division does not extend up to the point of fixation. Some implications for word recognition and reading are discussed. PMID:21559084

Deconstructing Calculation Methods, Part 4: Division

ERIC Educational Resources Information Center

Thompson, Ian

2008-01-01

In the final article of a series of four, the author deconstructs the primary national strategy's approach to written division. The approach to division is divided into five stages: (1) mental division using partition; (2) short division of TU / U; (3) "expanded" method for HTU / U; (4) short division of HTU / U; and (5) long division.…

Regulation of transcription of the cell division gene ftsA during sporulation of Bacillus subtilis.

PubMed Central

Gholamhoseinian, A; Shen, Z; Wu, J J; Piggot, P

1992-01-01

Three distinct 5' ends of ftsA mRNA were identified by S1 mapping and by primer extension analysis. These are thought to represent three transcription start sites. The transcripts from the downstream and upstream sites were detected throughout growth. The transcript from the middle site was not detected during exponential growth but was detected within 30 min of the start of sporulation, when it was the predominant transcript. Insertion of a cat cassette in the middle promoter, ftsAp2 (p2), did not affect vegetative growth but prevented postexponential symmetrical division and spore formation. Transcription from p2 was dependent on RNA polymerase containing sigma H, and promoter p2 resembled the consensus sigma H promoter. Transcription from p2 did not require expression of the spo0A, spo0B, spo0E, spo0F, or spo0K loci. Northern (RNA) blot analysis indicated that ftsA is cotranscribed with the adjacent ftsZ gene. Multiple promoters provide a mechanism by which essential vegetative genes can be subjected to sporulation control independent of control during vegetative growth. In the case of ftsA,Z, the promoters provide a mechanism to permit septum formation in conditions of nutrient depletion that might be expected to shut down the vegetative division machinery. Images PMID:1624452

49 CFR 239.7 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-10-01

... the Administrator's delegate. Control center means a central location on a railroad with....g., a superintendent, division manager, or equivalent), who has jurisdiction over a portion of the... passenger train emergency. Emergency response communications center means a central location, or a group of...

46 CFR 78.01-2 - Incorporation by reference.

Code of Federal Regulations, 2011 CFR

2011-10-01

...), and at the U.S. Coast Guard, Lifesaving and Fire Safety Division (CG-5214), 2100 2nd St., SW., Stop... Kingdom. Resolution A.654(16), Graphical Symbols for Fire Control Plans—78.45-1 [CGD 95-028, 62 FR 51204...

46 CFR 78.01-2 - Incorporation by reference.

Code of Federal Regulations, 2012 CFR

2012-10-01

...), and at the U.S. Coast Guard, Lifesaving and Fire Safety Division (CG-ENG-4), 2100 2nd St., SW., Stop... Kingdom. Resolution A.654(16), Graphical Symbols for Fire Control Plans—78.45-1 [CGD 95-028, 62 FR 51204...

46 CFR 78.01-2 - Incorporation by reference.

Code of Federal Regulations, 2010 CFR

2010-10-01

...), and at the U.S. Coast Guard, Lifesaving and Fire Safety Division (CG-5214), 2100 2nd St., SW., Stop... Kingdom. Resolution A.654(16), Graphical Symbols for Fire Control Plans—78.45-1 [CGD 95-028, 62 FR 51204...

76 FR 16802 - Extension of Approval of Information Collection, OMB Control Number 1004-0196

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-25

...'' regardless of the form of your comments. FOR FURTHER INFORMATION CONTACT: Barbara Gamble, Division of Fluid... Federal Information Relay Service (FIRS) at 1-800-877-8339, to contact Ms. Gamble. You may also review the...

75 FR 50775 - Extension of Approval of Information Collection, OMB Control Number 1004-0196

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-17

... INFORMATION CONTACT: To receive a copy of the information collection request, contact Barbara Gamble, Division... call the Federal Information Relay Service (FIRS) at 1- 800-877-8339, to leave a message for Ms. Gamble...

Annual Industrial Capabilities Report to Congress

DTIC Science & Technology

2008-03-01

61 5.1 Aircraft Sector Industrial Summary...basis, key contractor workforce capabilities necessary for successful programs. Industry segment-level baseline assessments ( aircraft ; command, control...monitored representative individual corporate divisions for each major industry segment ( aircraft parts and components, water craft, ground vehicles

An Overview Of Current Research At The Environmental Protection Agency

EPA Science Inventory

The purpose of this presentation is to provide an overview of the current research at the Environmental Protection Agency. FOR FURTHER INFORMATION, CONTACT ROGER B. YEARDLEY, JR., LAND REMEDIATION AND POLLUTION CONTROL DIVISION, 513-569-7548.

78 FR 37657 - Petition for Waiver of Compliance

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-21

... Division of the Regional Transportation Authority (Metra) and its operating company, the Northeast Illinois... CFR Part 236, Rules, Standards, and Instructions Governing the Installation, Inspection, Maintenance... each train operating in train stop, train control or cab signal territory; equipped. Specifically...

Reflectorized license plates : do they reduce nighttime rear-end collisions?.

DOT National Transportation Integrated Search

1974-01-01

The Commonwealth of Virginia randomly distributed 100,000 sets of experimental reflectorized and 100,000 sets of control nonreflective 1971 license plates, Each Division of Motor Vehicles distribution point in the state received and sold a pro rata n...

5 CFR 1312.31 - Security violations.

Code of Federal Regulations, 2010 CFR

2010-01-01

... States Secret Service when an office/division fails to properly secure classified information. Upon... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Security violations. 1312.31 Section 1312..., DOWNGRADING, DECLASSIFICATION AND SAFEGUARDING OF NATIONAL SECURITY INFORMATION Control and Accountability of...

40 CFR 62.7851 - Identification of sources.

Code of Federal Regulations, 2013 CFR

2013-07-01

... Air Pollution Control Division dated November 23, 2004, certifying that there are no existing sulfuric... Section 62.7851 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) APPROVAL AND PROMULGATION OF STATE PLANS FOR DESIGNATED FACILITIES AND POLLUTANTS New Mexico...

40 CFR 62.7851 - Identification of sources.

Code of Federal Regulations, 2014 CFR

2014-07-01

... Air Pollution Control Division dated November 23, 2004, certifying that there are no existing sulfuric... Section 62.7851 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) APPROVAL AND PROMULGATION OF STATE PLANS FOR DESIGNATED FACILITIES AND POLLUTANTS New Mexico...

40 CFR 62.7851 - Identification of sources.

Code of Federal Regulations, 2012 CFR

2012-07-01

... Air Pollution Control Division dated November 23, 2004, certifying that there are no existing sulfuric... Section 62.7851 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) APPROVAL AND PROMULGATION OF STATE PLANS FOR DESIGNATED FACILITIES AND POLLUTANTS New Mexico...

42 CFR 71.44 - Disinsection of aircraft.

Code of Federal Regulations, 2013 CFR

2013-10-01

... current list of approved insecticides and sources may be obtained from the Division of Quarantine, Center for Prevention Services, Centers for Disease Control, Atlanta, GA 30333. ... area that is infected with insect-borne communicable disease and the aircraft is suspected of harboring...

42 CFR 71.44 - Disinsection of aircraft.

Code of Federal Regulations, 2010 CFR

2010-10-01

... current list of approved insecticides and sources may be obtained from the Division of Quarantine, Center for Prevention Services, Centers for Disease Control, Atlanta, GA 30333. ... area that is infected with insect-borne communicable disease and the aircraft is suspected of harboring...

42 CFR 71.44 - Disinsection of aircraft.

Code of Federal Regulations, 2012 CFR

2012-10-01

... current list of approved insecticides and sources may be obtained from the Division of Quarantine, Center for Prevention Services, Centers for Disease Control, Atlanta, GA 30333. ... area that is infected with insect-borne communicable disease and the aircraft is suspected of harboring...

42 CFR 71.44 - Disinsection of aircraft.

Code of Federal Regulations, 2014 CFR

2014-10-01

... current list of approved insecticides and sources may be obtained from the Division of Quarantine, Center for Prevention Services, Centers for Disease Control, Atlanta, GA 30333. ... area that is infected with insect-borne communicable disease and the aircraft is suspected of harboring...

42 CFR 71.44 - Disinsection of aircraft.

Code of Federal Regulations, 2011 CFR

2011-10-01

... current list of approved insecticides and sources may be obtained from the Division of Quarantine, Center for Prevention Services, Centers for Disease Control, Atlanta, GA 30333. ... area that is infected with insect-borne communicable disease and the aircraft is suspected of harboring...

Accelerator Technology Division annual report, FY 1989

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1990-06-01

This paper discusses: accelerator physics and special projects; experiments and injectors; magnetic optics and beam diagnostics; accelerator design and engineering; radio-frequency technology; accelerator theory and simulation; free-electron laser technology; accelerator controls and automation; and high power microwave sources and effects.

DETERMINATION OF SORPTION PARAMETERS FOR 36 VOC/MATERIAL COMBINATIONS

EPA Science Inventory

EPA's Air Pollution Prevention and Control Division is currently investigating sorptive interactions (sink effects) of volatile organic compounds and semi-volatile organic compounds when exposed to common indoor surface materials. The objective is to recommend the best sink mode...

On-Site Construction Productivity Improvement Through Total Quality Management

DTIC Science & Technology

1991-12-01

morale of the work force. i Kaoru Ishikawa , Guide to Ouality Control, (2d ed; New York: Asian Productivity Organization, 1982), p. 45. ’"Ibid. 34...Curve Kaoru Ishikawa , Guide to Quality Control, (2d ed; New York: Asian Productivity Organization, 1982), p. 62. 54 For any normal distribution, 99.73...KAIZEN The Key to Japan’s Comoetitive Success. New York: Random House Business Division, 1986. Ishikawa , Kaoru , Guide to Ouality Control. 2d ed; New

Army Corps of Engineers, Southwestern Division, Reservoir Control Center Annual Report 1988

DTIC Science & Technology

1989-01-01

water control data system. This system includes the equipment and software used for the acquisition, transmission and processing of real-time hydrologic... transmission . The SWD system was installed at the Federal Center in Fort Worth, Texas, in September 1983. This is a Synergetics Model 10C direct Readout Ground...reservoir projects under control of the Department of the Army in the area comprising all of Arkansas and Louisiana and portions of Missouri, Kansas

Drone Control System

NASA Technical Reports Server (NTRS)

1983-01-01

Drones, subscale vehicles like the Firebees, and full scale retired military aircraft are used to test air defense missile systems. The DFCS (Drone Formation Control System) computer, developed by IBM (International Business Machines) Federal Systems Division, can track ten drones at once. A program called ORACLS is used to generate software to track and control Drones. It was originally developed by Langley and supplied by COSMIC (Computer Software Management and Information Center). The program saved the company both time and money.

Mechanical signals in plant development: a new method for single cell studies

NASA Technical Reports Server (NTRS)

Lynch, T. M.; Lintilhac, P. M.

1997-01-01

Cell division, which is critical to plant development and morphology, requires the orchestration of hundreds of intracellular processes. In the end, however, cells must make critical decisions, based on a discrete set of mechanical signals such as stress, strain, and shear, to divide in such a way that they will survive the mechanical loads generated by turgor pressure and cell enlargement within the growing tissues. Here we report on a method whereby tobacco protoplasts swirled into a 1.5% agarose entrapment medium will survive and divide. The application of a controlled mechanical load to agarose blocks containing protoplasts orients the primary division plane of the embedded cells. Photoelastic analysis of the agarose entrapment medium can identify the lines of principal stress within the agarose, confirming the hypothesis that cells divide either parallel or perpendicular to the principal stress tensors. The coincidence between the orientation of the new division wall and the orientation of the principal stress tensors suggests that the perception of mechanical stress is a characteristic of individual plant cells. The ability of a cell to determine a shear-free orientation for a new partition wall may be related to the applied load through the deformation of the matrix material. In an isotropic matrix a uniaxial load will produce a rotationally symmetric strain field, which will define a shear-free plane. Where high stress intensities combine with the loading geometry to produce multiaxial loads there will be no axis of rotational symmetry and hence no shear free plane. This suggests that two mechanisms may be orienting the division plane, one a mechanism that works in rotationally symmetrical fields, yielding divisions perpendicular to the compressive tensor, parallel to the long axis of the cell, and one in asymmetric fields, yielding divisions parallel to the short axis of the cell and the compressive tensor.