76 FR 36151 - Notice of Inventory Completion: Museum of Anthropology, University of Michigan, Ann Arbor, MI

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-21

... of Anthropology, University of Michigan, Ann Arbor, MI AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Museum of Anthropology, University of Michigan, has completed an inventory... the Museum of Anthropology, University of Michigan. Repatriation of the human remains to the tribe...

Evaluation Of The Advanced Operating System Of The Ann Arbor Transportation Authority : Driver And Dispatcher Perceptions Of AATA'S Advanced Operating System

DOT National Transportation Integrated Search

1999-01-01

In 1997, the Ann Arbor (Michigan) Transportation Authority began deploying advanced public transportation systems (APTS) technologies in its fixed route and paratransit operations. The project's concept is the integration of a range of such technolog...

76 FR 80392 - Notice of Inventory Completion: University of Michigan Museum of Anthropology, Ann Arbor, MI

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-23

...: University of Michigan Museum of Anthropology, Ann Arbor, MI AGENCY: National Park Service, Interior. ACTION... Michigan officials and its Museum of Anthropology professional staff in consultation with representatives... accessioned into the Museum of Anthropology. Between 2007 and 2009 the remains were inventoried at the...

77 FR 34991 - Notice of Inventory Completion: Museum of Anthropology, University of Michigan, Ann Arbor, MI...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-12

... DEPARTMENT OF THE INTERIOR National Park Service [NPS-WASO-NAGPRA-10375; 2200-1100-665] Notice of Inventory Completion: Museum of Anthropology, University of Michigan, Ann Arbor, MI; Correction AGENCY: National Park Service, Interior. ACTION: Notice; correction. Notice is here given in accordance with the...

Dover Schools' Unintelligent Design

ERIC Educational Resources Information Center

Barlow, Dudley

2006-01-01

The author of this article was surprised to read in the December 21, 2005, Ann Arbor News that "The Ann Arbor-based Thomas More Law Center, which represented the Dover [Pennsylvania] School District in its federal case for the teaching of intelligent design, has threatened to sue Gull Lake [Michigan] Community Schools over its policy that…

Evaluation Of The Advanced Operating System Of The Ann Arbor Transportation Authority : Transfer And On-Time Performance Study : Before And After AOS Implementation, October 1996 - May 1999

DOT National Transportation Integrated Search

1999-01-01

In 1997, the Ann Arbor (Michigan) Transportation Authority began deploying advanced public transportation systems (APTS) technologies in its fixed route and paratransit operations. The project's concept is the integration of a range of such technolog...

Evaluation Of The Advanced Operating System Of The Ann Arbor Transportation Authority : Cost Study : Before, During And After AOS Implementation (October 1996-May 1999)

DOT National Transportation Integrated Search

1999-01-01

In 1997, the Ann Arbor (Michigan) Transportation Authority (AATA) began deploying advanced public transportation systems (APTS) technologies in its fixed route and paratransit operations. The project's concept is the integration of a range of such te...

77 FR 66547 - Approval and Promulgation of Implementation Plans; Michigan; Detroit-Ann Arbor Nonattainment Area...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-06

... 2005 Base Year Emissions Inventory AGENCY: Environmental Protection Agency (EPA). ACTION: Final rule. SUMMARY: EPA is approving the fine particulate matter (PM 2.5 ) 2005 base year emissions inventory, a... 2005 base year emissions inventory for the Detroit-Ann Arbor area. EPA did not receive any comments...

The American Military on the Frontier

DTIC Science & Technology

1976-04-01

1783. Ncrvian: University of ÖFlahoma Press, HeT. (E OQ iC9 ^7^) Flison, John 1753?-17’𔄂. The discovery and settlement of Kentucke. Ann ...8217■" Fremont, John Charles, 1813-1890. Peport of the explorlmT expedition to the Pocky Ntountalns. Ann Arbor, Michigan: Uhlverslty Vlcrofllnis...O/erslze F 592 P63e) . Sources of the Mississippi and the V.’eytem Lculrlqm ’"terri- tory. Ann Arbor. ^Ich.: Ur.lversltv Microfilms

76 FR 73670 - Notice of Inventory Completion: University of Michigan Museum of Anthropology, Ann Arbor, MI

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-29

...: University of Michigan Museum of Anthropology, Ann Arbor, MI AGENCY: National Park Service, Interior. ACTION... Museum of Anthropology NAGPRA collections staff in consultation with representatives of the Bay Mills... Anthropology purchased the human remains from Reverend L. P. Rowland in November of 1924 as part of a larger...

Lenalidomide, Ibrutinib, and Rituximab in Treating Patients With Relapsed or Refractory Chronic Lymphocytic Leukemia or Small Lymphocytic Lymphoma That Is Metastatic or Cannot Be Removed by Surgery

ClinicalTrials.gov

2018-04-13

Ann Arbor Stage III Small Lymphocytic Lymphoma; Ann Arbor Stage IV Small Lymphocytic Lymphoma; Recurrent Chronic Lymphocytic Leukemia; Recurrent Small Lymphocytic Lymphoma; Refractory Chronic Lymphocytic Leukemia; Refractory Small Lymphocytic Lymphoma; Stage III Chronic Lymphocytic Leukemia; Stage IV Chronic Lymphocytic Leukemia

Proceedings of Workshop on Priority Great Lakes Environmental Research Initiatives (Great Lakes Environmental Research Laboratory, Ann Arbor, Michigan, October 10-11, 1974).

ERIC Educational Resources Information Center

Pinsak, Arthur P., Ed.

This publication contains the proceedings of a workshop held in Ann Arbor, Michigan to identify the priority Great Lakes environmental research initiatives. The five major objectives of the workshop include the determination of research initiatives, opportunities for university research communities to discuss and recommend future research…

An Intersection of Interests: The Millennial Generation and an Alternative World Language Teacher Education Program

ERIC Educational Resources Information Center

Morrissey, Gwynne E.; Coolican, Maria J.; Wolfgang, David F.

2011-01-01

The Ann Arbor Languages Partnership (A2LP) between Ann Arbor Public Schools and the University of Michigan's School of Education recruits Spanish-speaking undergraduates from many academic majors to teach Spanish in the district's 3rd and 4th grade classrooms during the academic year. The partnership allows the district to offer students a world…

Role of c-Src Activation on Prostate Cancer Lymph Node Metastases

DTIC Science & Technology

2008-10-01

dentistry Yonsei University College of Dentistry , Seoul, Korea D.D.S. 1995-1999 Dentistry Korea University Graduate School, Seoul, Korea M.S. 1999-2001...School of Dentistry , Ann Arbor, MI Postdoctoral training 2008- Present Cancer Biology A. Positions and Honors. Positions and Employment...Michigan School of Dentistry , Ann Arbor, MI Other Experience and Professional Memberships 2007- Associate Member, American Association for

Prostate Cancer Clinical Trials Group - The University of Michigan Site

DTIC Science & Technology

2014-06-01

Medicine and Urology University of Michigan Comprehensive Cancer Center Internal Medicine , Hematology Oncology 7314 Cancer Center, SPC 5946 Ann...Arbor, MI 48109-5946 mahahuss@umich.edu David C. Smith, M.D., FACP, Professor, Departments of Internal Medicine and Urology University of Michigan...Comprehensive Cancer Center Internal Medicine , Hematology Oncology 7302 Cancer Center, SPC 5946 Ann Arbor, MI 48109-5946 dcsmith@umich.edu

Deterring Spoilers: Peace Enforcement Operations and Political Settlements to Conflict

DTIC Science & Technology

2008-03-01

Intrastate Conflict ( Ann Arbor: University of Michigan Press), 14. 5 include improving human rights standards, military codes of conduct, and the...Pamela Aall (Washington, D.C.: United States Institute of Peace, 2001), 543. 6 different indicators. Peace support operations ( PSO ) is a general term...International Affairs 81 (2005): 325-39. Regan, Patrick M. Civil Wars and Foreign Powers: Outside Intervention in Intrastate Conflict. Ann Arbor

PDI Coamplified Genes in Ovarian Cancer

DTIC Science & Technology

2017-08-01

ORGANIZATION: University of Michigan Ann Arbor, MI 48109 REPORT DATE: August 2017 TYPE OF REPORT: Final PREPARED FOR: U.S. Army Medical Research ...Michigan Ann Arbor, MI 48109 9. SPONSORING / MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSOR/MONITOR’S ACRONYM(S) U.S. Army Medical Research and...distinct subtypes having different tissues of origin, diverse genetic landscapes, and respond differently to therapy.1-3 For example , serous EOC is

Tazemetostat in Treating Patients With Relapsed or Refractory Advanced Solid Tumors, Non-Hodgkin Lymphoma, or Histiocytic Disorders With EZH2, SMARCB1, or SMARCA4 Gene Mutations (A Pediatric MATCH Treatment Trial)

ClinicalTrials.gov

2018-06-20

Advanced Malignant Solid Neoplasm; Ann Arbor Stage III Childhood Hodgkin Lymphoma; Ann Arbor Stage III Childhood Non-Hodgkin Lymphoma; Ann Arbor Stage IV Childhood Hodgkin Lymphoma; Ann Arbor Stage IV Childhood Non-Hodgkin Lymphoma; Ewing Sarcoma/Peripheral Primitive Neuroectodermal Tumor; EZH2 Gain of Function; EZH2 Gene Mutation; Histiocytosis; Loss of BRG1 Protein Expression; Loss of INI 1 Protein Expression; Low Grade Glioma; Recurrent Childhood Central Nervous System Neoplasm; Recurrent Childhood Ependymoma; Recurrent Childhood Soft Tissue Sarcoma; Recurrent Ewing Sarcoma; Recurrent Glioma; Recurrent Hepatoblastoma; Recurrent Hodgkin Lymphoma; Recurrent Langerhans Cell Histiocytosis; Recurrent Malignant Germ Cell Tumor; Recurrent Malignant Glioma; Recurrent Malignant Solid Neoplasm; Recurrent Medulloblastoma; Recurrent Neuroblastoma; Recurrent Non-Hodgkin Lymphoma; Recurrent Osteosarcoma; Recurrent Peripheral Primitive Neuroectodermal Tumor; Recurrent Rhabdoid Tumor; Recurrent Rhabdomyosarcoma; Recurrent Soft Tissue Sarcoma; Refractory Central Nervous System Neoplasm; Refractory Hodgkin Lymphoma; Refractory Langerhans Cell Histiocytosis; Refractory Malignant Germ Cell Tumor; Refractory Malignant Glioma; Refractory Medulloblastoma; Refractory Neuroblastoma; Refractory Non-Hodgkin Lymphoma; Refractory Osteosarcoma; Refractory Peripheral Primitive Neuroectodermal Tumor; Refractory Rhabdoid Tumor; Refractory Soft Tissue Sarcoma; Rhabdoid Tumor; SMARCA4 Gene Inactivation; SMARCB1 Gene Inactivation; Stage III Soft Tissue Sarcoma AJCC v7; Stage IV Soft Tissue Sarcoma AJCC v7; Wilms Tumor

Josh Novacheck | NREL

Science.gov Websites

Arbor, MI, 2014 M.S. in natural resources and environment, University of Michigan, Ann Arbor, MI, 2014 ) Research Analyst, Center for Energy and Environment, Minneapolis, MN (2010-2012)

The Potential for Carbon Sequestration in the United States

DTIC Science & Technology

2007-09-01

Potential of U.S. Cropland to Sequester Carbon and Mitigate the Greenhouse Effect (Ann Arbor, Mich.: Ann Arbor Press, 1998), pp. 18–21; R.F. Follett and...others, The Potential of U.S. Grazing Land to Sequester Carbon and Mitigate the Greenhouse Effect (Boca Raton, Fla.: CRC Press, 2001), pp. 401–430... the Greenhouse Effect , pp. 18–21; R. Lal and others, “Managing U.S. Cropland to Sequester Carbon in Soil,” Journal of Soil and Water Conservation

Ocular adnexal diffuse large B-cell lymphoma: a multicenter international study.

PubMed

Munch-Petersen, Helga D; Rasmussen, Peter K; Coupland, Sarah E; Esmaeli, Bita; Finger, Paul T; Graue, Gerardo F; Grossniklaus, Hans E; Honavar, Santosh G; Khong, Jwu Jin; McKelvie, Penny A; Mulay, Kaustubh; Prause, Jan U; Ralfkiaer, Elisabeth; Sjö, Lene D; Sniegowski, Matthew C; Vemuganti, Geeta K; Heegaard, Steffen

2015-02-01

The clinical features of diffuse large B-cell lymphoma (DLBCL) subtype of ocular adnexal lymphoma have not previously been evaluated in a large cohort to our knowledge. To investigate the clinical features of ocular adnexal DLBCL (OA-DLBCL). This retrospective international cooperative study involved 6 eye cancer centers. During 30 years, 106 patients with OA-DLBCL were identified, and 6 were excluded from the study. The median follow-up period was 52 months. Overall survival, disease-specific survival (DSS), and progression-free survival were the primary end points. One hundred patients with OA-DLBCL were included in the study (median age, 70 years), of whom 54 (54.0%) were female. The following 3 groups of patients with lymphoma could be identified: primary OA-DLBCL (57.0%), OA-DLBCL and concurrent systemic lymphoma (29.0%), and ocular adnexal lymphoma relapse of previous systemic lymphoma (14.0%). Of 57 patients with primary OA-DLBCL, 53 (93.0%) had Ann Arbor stage IE disease, and 4 (7.0%) had Ann Arbor stage IIE disease. According to the TNM staging system, 43 of 57 (75.4%) had T2 tumors. Among all patients, the most frequent treatments were external beam radiation therapy with or without surgery (31.0%) and rituximab-cyclophosphamide, hydroxydaunorubicin, vincristine sulfate, prednisone (CHOP) or rituximab-CHOP-like chemotherapy with or without external beam radiation therapy (21.0%). The 5-year overall survival among the entire cohort was 36.0% (median, 3.5 years; 95% CI, 2.5-4.5 years). Relapse occurred in 43.9% (25 of 57) of patients with primary OA-DLBCL. Increasing T category of the TNM staging system was predictive of DSS (P = .04) in primary OA-DLBCL, whereas the Ann Arbor staging system was not. However, when taking all 100 patients into account, Ann Arbor stage was able to predict DSS (P = .01). Women had a longer median DSS than men (9.8 years; 95% CI, 1.9-17.7 years vs 3.3 years; 95% CI, 1.6-5.0; P = .03). Most patients with primary OA-DLBCL were seen with Ann Arbor stage IE and TNM T2 disease. The 5-year overall survival was between 2.5 and 4.5 years, which is the 95% CI around the median of 3.5 years in this cohort. Increasing T category appears to be associated with decreased DSS among patients with primary OA-DLBCL. When taking all patients into account, sex and Ann Arbor stage also seem to be DSS predictors.

Selumetinib in Treating Patients With Relapsed or Refractory Advanced Solid Tumors, Non-Hodgkin Lymphoma, or Histiocytic Disorders With Activating MAPK Pathway Mutations (A Pediatric MATCH Treatment Trial)

ClinicalTrials.gov

2018-06-25

Advanced Malignant Solid Neoplasm; Ann Arbor Stage III Childhood Non-Hodgkin Lymphoma; Ann Arbor Stage IV Childhood Non-Hodgkin Lymphoma; BRAF Gene Mutation; GNA11 Gene Mutation; GNAQ Gene Mutation; Histiocytosis; HRAS Gene Mutation; KRAS Gene Mutation; NF1 Gene Mutation; NRAS Gene Mutation; Recurrent Childhood Central Nervous System Neoplasm; Recurrent Childhood Non-Hodgkin Lymphoma; Recurrent Malignant Solid Neoplasm; Recurrent Neuroblastoma; Refractory Central Nervous System Neoplasm; Refractory Malignant Solid Neoplasm; Refractory Neuroblastoma; Refractory Non-Hodgkin Lymphoma

Ensartinib in Treating Patients With Relapsed or Refractory Advanced Solid Tumors, Non-Hodgkin Lymphoma, or Histiocytic Disorders With ALK or ROS1 Genomic Alterations (A Pediatric MATCH Treatment Trial)

ClinicalTrials.gov

2018-06-25

Advanced Malignant Solid Neoplasm; ALK Fusion Protein Expression; ALK Gene Mutation; ALK Gene Translocation; Ann Arbor Stage III Childhood Non-Hodgkin Lymphoma; Ann Arbor Stage IV Childhood Non-Hodgkin Lymphoma; Histiocytosis; Recurrent Childhood Central Nervous System Neoplasm; Recurrent Childhood Non-Hodgkin Lymphoma; Recurrent Malignant Solid Neoplasm; Recurrent Neuroblastoma; Refractory Central Nervous System Neoplasm; Refractory Malignant Solid Neoplasm; Refractory Neuroblastoma; Refractory Non-Hodgkin Lymphoma; ROS1 Fusion Positive; ROS1 Gene Mutation; ROS1 Gene Translocation

Workshop on Surface Science and Technology Held in Ann Arbor, Michigan on 7-9 November 1990

DTIC Science & Technology

1992-03-01

assess the state of the art of surface science and technology as well as to identify new research opportunities essential for the understanding and control...The objective of this workshop was to review and assess the state of the art of surface science and technology as well as to identify new research...AD-A253 566 ’ # 4 - m~~i n~nl lInIir ~~ na Ri1 ?epoi’rt: EN 1Workshop on Surface Science and Technology DTIC ft , L-CTE I OUG0 3192 Ann Arbor

Vemurafenib in Treating Patients With Relapsed or Refractory Advanced Solid Tumors, Non-Hodgkin Lymphoma, or Histiocytic Disorders With BRAF V600 Mutations (A Pediatric MATCH Treatment Trial)

ClinicalTrials.gov

2018-06-25

Advanced Malignant Solid Neoplasm; Ann Arbor Stage III Childhood Non-Hodgkin Lymphoma; Ann Arbor Stage IV Childhood Non-Hodgkin Lymphoma; BRAF NP_004324.2:p.V600X; Ependymoma; Ewing Sarcoma; Hepatoblastoma; Histiocytosis; Langerhans Cell Histiocytosis; Malignant Germ Cell Tumor; Malignant Glioma; Osteosarcoma; Peripheral Primitive Neuroectodermal Tumor; Recurrent Childhood Central Nervous System Neoplasm; Recurrent Childhood Non-Hodgkin Lymphoma; Recurrent Malignant Solid Neoplasm; Recurrent Neuroblastoma; Refractory Central Nervous System Neoplasm; Refractory Malignant Solid Neoplasm; Refractory Neuroblastoma; Refractory Non-Hodgkin Lymphoma; Rhabdoid Tumor; Rhabdomyosarcoma; Soft Tissue Sarcoma; Wilms Tumor

76 FR 44947 - Notice of Intent To Repatriate Cultural Items: University of Michigan Museum of Anthropology, Ann...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-27

... the appropriate Indian tribe, has determined that the items meet the definition of sacred objects and.... Representatives of any Indian tribe that believes itself to be culturally affiliated with the sacred objects may... of Anthropology, Ann Arbor, MI, that meet the definition of sacred objects under 25 U.S.C. 3001. This...

Paige Jadun | NREL

Science.gov Websites

dynamics. She has performed research in sustainable mobility, network optimization, supply chain analysis Experience Supply Chain Design Consultant, LLamasoft, Ann Arbor, MI Featured Publications Laura J

AATA web survey

DOT National Transportation Integrated Search

1999-01-01

During 1997, visitors to the Ann Arbor Transportation Authority's worldwide web site were : invited to complete an electronic questionnaire about their experience with the site. Eighty surveys were collected, representing a non-scientific self-select...

Annual ARC Program Review

Science.gov Websites

management of power and energy within vehicles, mobility and survivability of the complete vehicle system : University of Michigan, Ann Arbor, MI 48109 Registration is now closed as full venue capacity has been

Negotiation in the New Strategic Environment: Lessons From Iraq

DTIC Science & Technology

2007-08-01

Andrew Terrell , Reconstructing Iraq: Insights, Challenges, and Missions for Military Forces in a Post- Conflict Scenario, Carlisle Barracks, PA...Negotiation, Ann Arbor, MI: University of Michigan Press, 2000, pp. 271-273 (hereafter Zartman and Rubin, Symmetry and Assymmetry). 80. As discussed...122. See Mnookin, Beyond Winning, pp. 28-31. 123. See Anne L. Lytle, Jeanne M. Brett, and Debra L. Shapiro, “The Strategic Use of Interests, Rights

Impacts of an advanced public transportation system : demonstration project

DOT National Transportation Integrated Search

1999-10-01

In 1997, the Ann Arbor (Michigan) Transportation Authority began deploying a set of integrated : advanced public transportation system technologies in its vehicles, stations and control center. This paper summarizes selected findings of a multidimens...

Music's Open Secret.

ERIC Educational Resources Information Center

Wilson, Robin

2002-01-01

Explores how sexual harassment has long been a problem in music departments, and that two cases at top public colleges, the University of Michigan Ann Arbor and the University of Texas Austin, are drawing attention to the issue. (EV)

78 FR 65376 - Notice of Inventory Completion: University of Michigan, Ann Arbor, MI

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-31

... operator found the remains in displaced dirt during construction activities associated with a housing... excavation. A backhoe had removed some of the remains. These were collected from the backfill dirt...

Red Blood Cell Antibody Screen

MedlinePlus

... Internet]. Ann Arbor (MI): The Regents of the University of Michigan; c1995-2017. Coombs Antibody Test (Indirect ... gov/health/health-topics/topics/bdt/with NorthShore University Health System [Internet]. NorthShore University Health System; c2017. ...

Space Power Integration: Perspectives from Space Weapons Officers

DTIC Science & Technology

2006-12-01

staff at Air University Press, Dr. Philip Adkins, Mrs. Sherry Terrell , and Mrs. Vivian O’Neal. Their creation of an integrated book from nine...Techniques of Complex Systems Science: An Overview ( Ann Arbor, MI: Center for the Study of Complex Sys- tems, University of Michigan, 9 July 2003), 34...Depart- ment of the Navy Space Policy, 26 August 1993. Shalizi, Cosma Rohilla. Methods and Techniques of Complex Systems Science: An Overview. Ann

Alternative Fuels Data Center: Hydraulic Hybrids: A Success in Ann Arbor

Science.gov Websites

off with fuel savings, lower maintenance costs, and increased productivity. American Recovery and regenerative braking system also means huge savings in brake maintenance. Normally, a truck that stops and

Development of performance measures for non-motorized dynamics.

DOT National Transportation Integrated Search

2013-12-27

This report recommends performance measures for non-motorized (pedestrian and bicyclists) : traffic safety for Michigan cities. Based on the data collected from four Michigan cities, Ann : Arbor, East Lansing, Flint, and Grand Rapids, the research te...

A Graduate Course in Energy Conservation.

ERIC Educational Resources Information Center

Fickes, Michael

1999-01-01

Examines the University of Michigan's (Ann Arbor) success with a six-year energy conservation program (The Energy Star Program) offered by the Environmental Protection Agency. Describes the program's components and areas of savings the university has achieved. (GR)

40 CFR 59.213 - Incorporations by reference.

Code of Federal Regulations, 2010 CFR

2010-07-01

...., Washington, DC 20460, the EPA Library (MD-35), U.S. EPA, Research Triangle Park, NC 27711, or at the National... University Microfilms International, 300 North Zeeb Road, Ann Arbor MI, 48106. (1) ASTM Method E220-86...

75 FR 12175 - Application(s) for Duty-Free Entry of Scientific Instruments

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-15

..., 1301 Beal Avenue, Ann Arbor, MI 49109-2122. Instrument: Tester for TFT Imager. Manufacturer: Siemens AG... capability of amorphous silicon TFT and organic photo-diode. This instrument must be capable of measuring...

78 FR 65382 - Notice of Inventory Completion: University of Michigan, Ann Arbor, MI

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-31

... Lake Superior Chippewa Indians of Wisconsin; Red Lake Band of Chippewa Indians, Minnesota; Sac & Fox Nation of Missouri in Kansas and Nebraska; Sac & Fox Nation, Oklahoma; Sac & Fox Tribe of the Mississippi...

Olaparib in Treating Patients With Relapsed or Refractory Advanced Solid Tumors, Non-Hodgkin Lymphoma, or Histiocytic Disorders With Defects in DNA Damage Repair Genes (A Pediatric MATCH Treatment Trial)

ClinicalTrials.gov

2018-06-25

Advanced Malignant Solid Neoplasm; Ann Arbor Stage III Childhood Non-Hodgkin Lymphoma; Ann Arbor Stage IV Childhood Non-Hodgkin Lymphoma; Deleterious ATM Gene Mutation; Deleterious BRCA1 Gene Mutation; Deleterious BRCA2 Gene Mutation; Deleterious RAD51C Gene Mutation; Deleterious RAD51D Gene Mutation; Histiocytosis; Low Grade Glioma; Malignant Glioma; Recurrent Childhood Central Nervous System Neoplasm; Recurrent Childhood Ependymoma; Recurrent Childhood Malignant Germ Cell Tumor; Recurrent Childhood Non-Hodgkin Lymphoma; Recurrent Childhood Rhabdomyosarcoma; Recurrent Childhood Soft Tissue Sarcoma; Recurrent Ewing Sarcoma/Peripheral Primitive Neuroectodermal Tumor; Recurrent Glioma; Recurrent Hepatoblastoma; Recurrent Langerhans Cell Histiocytosis; Recurrent Malignant Solid Neoplasm; Recurrent Medulloblastoma; Recurrent Neuroblastoma; Recurrent Osteosarcoma; Refractory Central Nervous System Neoplasm; Refractory Langerhans Cell Histiocytosis; Refractory Malignant Solid Neoplasm; Refractory Neuroblastoma; Refractory Non-Hodgkin Lymphoma; Rhabdoid Tumor; Wilms Tumor

Erdafitinib in Treating Patients With Relapsed or Refractory Advanced Solid Tumors, Non-Hodgkin Lymphoma, or Histiocytic Disorders With FGFR Mutations (A Pediatric MATCH Treatment Trial)

ClinicalTrials.gov

2018-06-25

Advanced Malignant Solid Neoplasm; Ann Arbor Stage III Childhood Non-Hodgkin Lymphoma; Ann Arbor Stage IV Childhood Non-Hodgkin Lymphoma; FGFR1 Gene Mutation; FGFR2 Gene Mutation; FGFR3 Gene Mutation; FGFR4 Gene Mutation; Histiocytosis; Low Grade Glioma; Malignant Glioma; Recurrent Central Nervous System Neoplasm; Recurrent Childhood Ependymoma; Recurrent Childhood Malignant Germ Cell Tumor; Recurrent Childhood Medulloblastoma; Recurrent Childhood Non-Hodgkin Lymphoma; Recurrent Childhood Rhabdomyosarcoma; Recurrent Childhood Soft Tissue Sarcoma; Recurrent Ewing Sarcoma/Peripheral Primitive Neuroectodermal Tumor; Recurrent Hepatoblastoma; Recurrent Langerhans Cell Histiocytosis; Recurrent Malignant Solid Neoplasm; Recurrent Neuroblastoma; Recurrent Osteosarcoma; Refractory Central Nervous System Neoplasm; Refractory Langerhans Cell Histiocytosis; Refractory Malignant Solid Neoplasm; Refractory Neuroblastoma; Refractory Non-Hodgkin Lymphoma; Rhabdoid Tumor; Stage III Soft Tissue Sarcoma AJCC v7; Stage IV Soft Tissue Sarcoma AJCC v7; Wilms Tumor

School day and weekend patterns of physical activity in urban 11-year-olds: a cross-cultural comparison.

PubMed

Jurak, Gregor; Sorić, Maroje; Starc, Gregor; Kovač, Marjeta; Mišigoj-Duraković, Marjeta; Borer, Katarina; Strel, Janko

2015-01-01

This multi-center study was conducted to objectively evaluate energy expenditure and physical activity (PA) patterns on school days and weekends in urban 11-year-olds. The sample consisted of 241 children from three cities: Zagreb, Ljubljana (both in Central Europe) and Ann Arbor (United States). Energy expenditure and PA were assessed during two school days and two weekend days using a multiple-sensor body monitor. Differences between the cities were observed for all PA variables. The highest level of moderate to vigorous PA (MVPA) was noted in Ljubljana boys [284 (98) min/day] and the lowest in Zagreb girls [179 (95) min/day]. In Zagreb and Ljubljana, boys were more physically active than girls, while in Ann Arbor the opposite was observed. In contrast, no gender difference in sedentary behavior was observed in any of the cities. A decline in PA from school days to weekends was noted in all city groups in both genders. However, the magnitude of the reduction in daily energy expenditure differed between the cities, with the largest differences being observed in Ljubljana and the smallest in Ann Arbor. In all three city groups, the great majority of boys and girls achieved current recommendations of 60 min of MVPA either during school days or weekends. Weekends seem to be an appropriate target when promoting PA in 11-year-olds in all the cities included in the study. Increasing vigorous activity on weekends seems to be of particular importance in Zagreb and Ljubljana. © 2014 Wiley Periodicals, Inc.

The University of Michigan, Kellogg Building Expansion & Renovation, Ann Arbor, Michigan.

ERIC Educational Resources Information Center

Design Cost Data, 2001

2001-01-01

Presents design, construction, and cost data for the University of Michigan's Kellogg Building expansion and renovation project. A list of project manufacturers and suppliers is provided along with four photographs and four floor plans. (GR)

Evaluation of Automatic Vehicle Location accuracy

DOT National Transportation Integrated Search

1999-01-01

This study assesses the accuracy of the Automatic Vehicle Location (AVL) data provided for the buses of the Ann Arbor Transportation Authority with Global Positioning System (GPS) technology. In a sample of eighty-nine bus trips two kinds of accuracy...

78 FR 65371 - Notice of Inventory Completion: University of Michigan, Ann Arbor, MI

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-31

... sand removal operations near Exeter Road. The remains were taken to a biology teacher at Monroe High... relationship of shared group identity cannot be reasonably traced between the Native American human remains and...

78 FR 65367 - Notice of Inventory Completion: University of Michigan, Ann Arbor, MI

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-31

... landowner was constructing a basement and spreading displaced dirt in his nearby orchard when he discovered... one adult and an unspecified number of associated funerary objects from out of the displaced dirt. The...

75 FR 5967 - Procurement List; Additions and Deletions

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-05

...., Fresno, CA 3971 RESEARCH PARK DRIVE, ANN ARBOR, MI 22600 HALL ROAD, CLINTON TOWNSHIP, MI 477 MICHIGAN AVE... COUNTRY CLUB RD, EUGENE, OR GUS J. SOLOMON CTHSE: 620 SW MAIN ST, PORTLAND, OR E.GREEN--W.WYATT FB: 1220...

Holocene precipitation in the coastal temperate rainforest complex of southern British Columbia, Canada

NASA Astrophysics Data System (ADS)

Brown, K. J.; Fitton, R. J.; Schoups, G.; Allen, G. B.; Wahl, K. A.; Hebda, R. J.

2006-11-01

Pollen data from 69 surface samples from Vancouver Island, Canada, were used to develop a ratio index of precipitation, Douglas fir-western hemlock index (DWHI). DWHI ratios were combined with interpolated estimates of mean annual precipitation to develop pollen-based precipitation transfer functions. The optimal regression model, with a predictive range of 960-2600 mm, was applied to 10 Holocene lake sediment records distributed across a ˜150 km long coastal-inland precipitation gradient. Predicted precipitation was spatially modelled in a geographic information system to examine the spatio-temporal history of precipitation from this representative portion of the coastal temperate rainforest (CTR) complex of western North America. The reconstructions show widespread early Holocene dry conditions coupled with a steep east-west precipitation gradient. Thereafter, the modern precipitation gradient established 7000 years ago, illustrating that the CTR complex has experienced marked short-distance east-west changes in precipitation in the past. Changes in the abundance of arboreal and non-arboreal vegetation, as well as fire disturbance, are often concomitant with changes in Holocene precipitation. Given the precipitation and vegetation history of the region, conservation initiatives should focus on the moist outer coastal zone since it appears to have the greatest amount of resilience to perturbations in precipitation, whereas monitoring programs for signs of climate change should be initiated in central and eastern areas as they appear sensitive to changes in the moisture regime.

Nuclear Astrophysics Before 1957

NASA Astrophysics Data System (ADS)

Salpeter, Edwin E.

I discuss especially my summer with Willy Fowler at Kellogg Radiation Laboratory in 1951, where I did my `triple alpha' work. I also go back even earlier to Arthur Eddington and Hans Bethe. The 1953 summer school in Ann Arbor only gets a mention.

40 CFR 65.13 - Incorporation by reference.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Zeeb Road, Ann Arbor, Michigan 48106. (1) ASTM D1946-77, Standard Method for Analysis of Reformed Gas by Gas Chromatography, IBR approved December 14, 2000 for §§ 65.64(e)(2) and 65.147(a)(4)(i) and (b...

40 CFR 65.13 - Incorporation by reference.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Zeeb Road, Ann Arbor, Michigan 48106. (1) ASTM D1946-77, Standard Method for Analysis of Reformed Gas by Gas Chromatography, IBR approved December 14, 2000 for §§ 65.64(e)(2) and 65.147(a)(4)(i) and (b...

Untangling the Strands of the Fourteenth Amendment.

ERIC Educational Resources Information Center

Lupu, Ira C.

1979-01-01

Explores trends in the Court's interpretation of the libertarian and egalitarian dimensions of the Fourteenth Amendment and offers a theory of the two strands. Available from Michigan Law Review, Hutchins Hall, Ann Arbor, MI 48109; single issues $3.50. (Author/IRT)

Reallocation: Strategies for Effective Resource Management.

ERIC Educational Resources Information Center

Hyatt, James A.; And Others

Retrenchment and reallocation processes at five public colleges and universities were studied: the University of Washington; University of Idaho; University of Michigan, Ann Arbor; Michigan State University; and Seattle Community College District. After an overview of institutional approaches to retrenchment and reallocation, the five individual…

Integrated Dial-A-Ride and Fixed Route Transit in Ann Arbor, Michigan

DOT National Transportation Integrated Search

1977-03-01

The report describes the development, implementation, and current status of the Teltran system as it existed in the spring of 1976. In particular, this evaluation provides a detailed description of Teltran system configuration (and changes in that co...

Identification of an amino acid residue on influenza C virus M1 protein responsible for formation of the cord-like structures of the virus.

PubMed

Muraki, Yasushi; Washioka, Hiroshi; Sugawara, Kanetsu; Matsuzaki, Yoko; Takashita, Emi; Hongo, Seiji

2004-07-01

Influenza C virus-like particles (VLPs) have been generated from cloned cDNAs. A cDNA of the green fluorescent protein (GFP) gene in antisense orientation was flanked by the 5' and 3' non-coding regions of RNA segment 5 of the influenza C virus. The cDNA cassette was inserted between an RNA polymerase I promoter and terminator of the Pol I vector. This plasmid DNA was transfected into 293T cells together with plasmids encoding virus proteins of C/Ann Arbor/1/50 or C/Yamagata/1/88. Transfer of the supernatants of the transfected 293T cells to HMV-II cells resulted in GFP expression in the HMV-II cells. The quantification of the GFP-positive HMV-II cells indicated the presence of approximately 10(6) VLPs (ml supernatant)(-1). Cords 50-300 microm in length were observed on transfected 293T cells, although the cords were not observed when the plasmid for M1 protein of C/Ann Arbor/1/50 was replaced with that of C/Taylor/1233/47. A series of transfection experiments with plasmids encoding M1 mutants of C/Ann Arbor/1/50 or C/Taylor/1233/47 showed that an amino acid at residue 24 of the M1 protein is responsible for cord formation. This finding provides direct evidence for a previous hypothesis that M1 protein is involved in the formation of cord-like structures protruding from the C/Yamagata/1/88-infected cells. Evidence was obtained by electron microscopy that transfected cells bearing cords produced filamentous VLPs, suggesting the potential role of the M1 protein in determining the filamentous/spherical morphology of influenza C virus.

The cold adapted and temperature sensitive influenza A/Ann Arbor/6/60 virus, the master donor virus for live attenuated influenza vaccines, has multiple defects in replication at the restrictive temperature

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chan, Winnie; Zhou, Helen; Kemble, George

2008-10-25

We have previously determined that the temperature sensitive (ts) and attenuated (att) phenotypes of the cold adapted influenza A/Ann Arbor/6/60 strain (MDV-A), the master donor virus for the live attenuated influenza A vaccines (FluMist), are specified by the five amino acids in the PB1, PB2 and NP gene segments. To understand how these loci control the ts phenotype of MDV-A, replication of MDV-A at the non-permissive temperature (39 deg. C) was compared with recombinant wild-type A/Ann Arbor/6/60 (rWt). The mRNA and protein synthesis of MDV-A in the infected MDCK cells were not significantly reduced at 39 deg. C during amore » single-step replication, however, vRNA synthesis was reduced and the nuclear-cytoplasmic export of viral RNP (vRNP) was blocked. In addition, the virions released from MDV-A infected cells at 39 deg. C exhibited irregular morphology and had a greatly reduced amount of the M1 protein incorporated. The reduced M1 protein incorporation and vRNP export blockage correlated well with the virus ts phenotype because these defects could be partially alleviated by removing the three ts loci from the PB1 gene. The virions and vRNPs isolated from the MDV-A infected cells contained a higher level of heat shock protein 70 (Hsp70) than those of rWt, however, whether Hsp70 is involved in thermal inhibition of MDV-A replication remains to be determined. Our studies demonstrate that restrictive replication of MDV-A at the non-permissive temperature occurs in multiple steps of the virus replication cycle.« less

Robot Training Through Incremental Learning

DTIC Science & Technology

2011-04-18

Turing Associates, Ann Arbor, MI 48103 ABSTRACT The real world is too complex and variable to directly program an autonomous ground robot’s...11 th Conf. Uncertainty in Artificial Intelligence, 338-45 (1995). [6] J. Cleary and L. Trigg, “K*: An Instance-based learner using an entropic

77 FR 1975 - Safety Advisory: Unauthorized Marking of Compressed Gas Cylinders

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-12

... Jackson Plaza, Ann Arbor, MI improperly requalified and marked high pressure compressed gas cylinders... DOT specification cylinders after its authority to requalifiy high pressure cylinders expired on... that Spears Fire & Safety continued to requalify and mark high pressure cylinders after their authority...

Tracking the deployment of the integrated metropolitan ITS infrastructure in Detroit, Ann Arbor : FY99 results

DOT National Transportation Integrated Search

2000-01-01

In January 1996, Secretary Pea set a goal of deploying the integrated metropolitan Intelligent Transportation System (ITS) infrastructure in 75 of the nations largest metropolitan areas by 2006. In 1997, the U.S. Department of Transportation ini...

Meeting report for Odd Pols 2016: Ann Arbor 2.0.

PubMed

Roy-Engel, Astrid M

2017-05-15

The Tenth International Conference on Transcription by RNA Polymerases I, III, IV and V (the 'Odd Pols') was held June 24-28, 2016 at the University of Michigan, Ann Arbor, USA and organized by David Engelke, Deborah Johnson, Richard Maraia, Lawrence Rothblum, David Schneider, Andrzej Wierzbicki and Astrid Engel. The organizers are grateful for the support from the Rackham Graduate School of the University of Michigan for providing the meeting venue. The environment provided a great background with unexpected encounters with fireflies, free live music and a festive fireworks display. The meeting was composed of eleven oral sessions and a poster session. The keynote speaker, Dave Engelke, opened the meeting with his presentation entitled "A personal history of pol III transcription - how we got here from the 'good old days'." The meeting drew attendees from sixteen countries that shared their research discoveries. Here we present some of the highlights from the meeting using summaries provided by the participants. Copyright © 2016 Elsevier B.V. All rights reserved.

Genetic engineering of live attenuated influenza viruses.

PubMed

Jin, Hong; Chen, Zhongying; Liu, Jonathan; Kemble, George

2012-01-01

The first live attenuated influenza vaccine (LAIV) was licensed in the USA in 2003; it is a trivalent vaccine composed of two type A (H1N1 and H3N2) and one type B influenza virus each at 10(7) fluorescent focus units (FFU). Each influenza vaccine strain is a reassortant virus that contains the hemagglutinin (HA) and neuraminidase (NA) gene segments from a wild-type influenza virus and the six internal protein gene segments from a master donor virus (MDV) of either cold-adapted A/Ann Arbor/6/60 or B/Ann Arbor/1/66. MDV confers the cold-adapted, temperature-sensitive, and attenuation phenotypes to the vaccine strains. The reassortant vaccine seeds are currently produced by reverse genetics and amplified in specific pathogen-free (SPF) 9-11 days old embryonated chicken eggs for manufacture. In addition, MDCK cell culture manufacture processes have been developed to produce LAIV for research use and with modifications for clinical and/or commercial grade material production.

Targeted Therapy Directed by Genetic Testing in Treating Pediatric Patients With Relapsed or Refractory Advanced Solid Tumors, Non-Hodgkin Lymphomas, or Histiocytic Disorders (The Pediatric MATCH Screening Trial)

ClinicalTrials.gov

2018-06-25

Advanced Malignant Solid Neoplasm; Ann Arbor Stage III Childhood Non-Hodgkin Lymphoma; Ann Arbor Stage IV Childhood Non-Hodgkin Lymphoma; Childhood Langerhans Cell Histiocytosis; Histiocytic Sarcoma; Juvenile Xanthogranuloma; Malignant Glioma; Recurrent Central Nervous System Neoplasm; Recurrent Childhood Ependymoma; Recurrent Childhood Malignant Germ Cell Tumor; Recurrent Childhood Medulloblastoma; Recurrent Childhood Non-Hodgkin Lymphoma; Recurrent Childhood Rhabdomyosarcoma; Recurrent Childhood Soft Tissue Sarcoma; Recurrent Ewing Sarcoma; Recurrent Glioma; Recurrent Hepatoblastoma; Recurrent Langerhans Cell Histiocytosis; Recurrent Malignant Solid Neoplasm; Recurrent Neuroblastoma; Recurrent Osteosarcoma; Recurrent Peripheral Primitive Neuroectodermal Tumor; Refractory Central Nervous System Neoplasm; Refractory Childhood Malignant Germ Cell Tumor; Refractory Langerhans Cell Histiocytosis; Refractory Malignant Solid Neoplasm; Refractory Neuroblastoma; Rhabdoid Tumor; Stage III Osteosarcoma AJCC v7; Stage III Soft Tissue Sarcoma AJCC v7; Stage IV Osteosarcoma AJCC v7; Stage IV Soft Tissue Sarcoma AJCC v7; Stage IVA Osteosarcoma AJCC v7; Stage IVB Osteosarcoma AJCC v7; Wilms Tumor

Larotrectinib in Treating Patients With Relapsed or Refractory Advanced Solid Tumors, Non-Hodgkin Lymphoma, or Histiocytic Disorders With NTRK Fusions (A Pediatric MATCH Treatment Trial)

ClinicalTrials.gov

2018-06-25

Advanced Malignant Solid Neoplasm; Ann Arbor Stage III Childhood Non-Hodgkin Lymphoma; Ann Arbor Stage IV Childhood Non-Hodgkin Lymphoma; Malignant Glioma; NTRK1 Fusion Positive; NTRK2 Fusion Positive; NTRK3 Fusion Positive; Recurrent Central Nervous System Neoplasm; Recurrent Childhood Ependymoma; Recurrent Childhood Malignant Germ Cell Tumor; Recurrent Childhood Medulloblastoma; Recurrent Childhood Non-Hodgkin Lymphoma; Recurrent Childhood Rhabdomyosarcoma; Recurrent Childhood Soft Tissue Sarcoma; Recurrent Ewing Sarcoma; Recurrent Glioma; Recurrent Hepatoblastoma; Recurrent Langerhans Cell Histiocytosis; Recurrent Malignant Solid Neoplasm; Recurrent Neuroblastoma; Recurrent Osteosarcoma; Recurrent Peripheral Primitive Neuroectodermal Tumor; Refractory Central Nervous System Neoplasm; Refractory Childhood Malignant Germ Cell Tumor; Refractory Langerhans Cell Histiocytosis; Refractory Malignant Solid Neoplasm; Refractory Neuroblastoma; Refractory Non-Hodgkin Lymphoma; Rhabdoid Tumor; Stage III Osteosarcoma AJCC v7; Stage III Soft Tissue Sarcoma AJCC v7; Stage IV Osteosarcoma AJCC v7; Stage IV Soft Tissue Sarcoma AJCC v7; Stage IVA Osteosarcoma AJCC v7; Stage IVB Osteosarcoma AJCC v7; Wilms Tumor

PI3K/mTOR Inhibitor LY3023414 in Treating Patients With Relapsed or Refractory Advanced Solid Tumors, Non-Hodgkin Lymphoma, or Histiocytic Disorders With TSC or PI3K/MTOR Mutations (A Pediatric MATCH Treatment Trial)

ClinicalTrials.gov

2018-06-18

Advanced Malignant Solid Neoplasm; Ann Arbor Stage III Non-Hodgkin Lymphoma; Ann Arbor Stage IV Non-Hodgkin Lymphoma; Malignant Glioma; Recurrent Central Nervous System Neoplasm; Recurrent Childhood Ependymoma; Recurrent Ewing Sarcoma; Recurrent Glioma; Recurrent Hepatoblastoma; Recurrent Langerhans Cell Histiocytosis; Recurrent Malignant Germ Cell Tumor; Recurrent Malignant Solid Neoplasm; Recurrent Medulloblastoma; Recurrent Neuroblastoma; Recurrent Non-Hodgkin Lymphoma; Recurrent Osteosarcoma; Recurrent Peripheral Primitive Neuroectodermal Tumor; Recurrent Rhabdomyosarcoma; Recurrent Soft Tissue Sarcoma; Refractory Central Nervous System Neoplasm; Refractory Langerhans Cell Histiocytosis; Refractory Malignant Germ Cell Tumor; Refractory Malignant Solid Neoplasm; Refractory Neuroblastoma; Refractory Non-Hodgkin Lymphoma; Rhabdoid Tumor; Stage III Osteosarcoma AJCC v7; Stage III Soft Tissue Sarcoma AJCC v7; Stage IV Osteosarcoma AJCC v7; Stage IV Soft Tissue Sarcoma AJCC v7; Stage IVA Osteosarcoma AJCC v7; Stage IVB Osteosarcoma AJCC v7; TSC1 Gene Mutation; TSC2 Gene Mutation; Wilms Tumor

Theory Y Is Not Participative Management

ERIC Educational Resources Information Center

Morton, Donald J.

1975-01-01

Participative management is not the most fruitful way to use Theory Y; much greater benefits can be obtained by stressing job characteristics that cultivate individual psychological desires. (Available from Office of Publications, Graduate School of Business Administration, University of Michigan, Ann Arbor, MI 48104, $2.50, single issue)…

Male-Female Differences in Perceived Organizational Legitimacy

ERIC Educational Resources Information Center

Bedeian, Arthur G.; Armenakis, Achilles A.

1975-01-01

Members of both sexes seemed to recognize that certain legitimate areas of organizational influence do exist; however, they disagreed somewhat on both the proper extent and composition of this influence. (Available from Office of Publications, Graduate School of Business Administration, University of Michigan, Ann Arbor, MI 48104, $2.50, single…

Black English and Reluctant Judges.

ERIC Educational Resources Information Center

Glazer, Nathan

1981-01-01

Discusses "Martin Luther King Jr. Elementary School Children, et al. v Ann Arbor School District Board," a court decision requiring that teachers be instructed in the role and significance of Black English. Argues that the issues of educational achievement and Black English do not constitute a matter for the courts. (GC)

John F. Geisz | NREL

Science.gov Websites

of Michigan (Ann Arbor). Geisz joined NREL in 1995 where he has been studying the OMVPE growth and , including dilute nitrogen and boron containing III-V alloys, III-V growth on silicon, and lattice-mismatched growth. He has developed three- and four-junction inverted metamorphic concentrator solar cells and

Improving Vocational Education in Post-High School Institutions. Final Report.

ERIC Educational Resources Information Center

MacArthur, Earl W.

Four ongoing programs in postsecondary vocational-technical education were examined in a national institute attended by 59 representatives from 31 states. Institutions reporting programs were: (1) Los Angeles Trade and Technical College, California, (2) Washtenaw Community College, Ann Arbor, Michigan, (3) Rockingham Community College, Wentworth,…

Orbiting Carbon Observatory Briefing

NASA Image and Video Library

2009-01-29

Anna Michalak, an Orbiting Carbon Observatory science team member from the University of Michigan, Ann Arbor, speaks during a media briefing to discuss the upcoming Orbiting Carbon Observatory mission, the first NASA spacecraft dedicated to studying carbon dioxide, Thursday, Jan. 29, 2009, at NASA Headquarters in Washington. Photo Credit: (NASA/Paul E. Alers)

75 FR 54652 - Notice Pursuant to the National Cooperative Research and Production Act of 1993-National Center...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-08

... Network Enterprises, Inc., Dallas, TX; Packer Engineering, Inc., Naperville, IL; PDQ Precision Inc..., Inc., Auburn Hills, MI; Pratt & Miller Engineering and Fabrication, Inc., New Hudson, MI; Seica Inc... Environmental, Inc., Plymouth, MI; Electrical-Mechanical Associates, Inc., Ann Arbor, MI; The Euclid Chemical...

Evaluation of a New Fracture Toughness Measuring Technique, and Adaptation of the Technique to Use Ultra-Small Specimens

DTIC Science & Technology

1979-09-01

University of Michigan, 2046 East Engineering, Ann Arbor, Michigan 48109 1 Dr. R.Foye, Army Research and Technology Lab, Air Movility R&D Lab, Ames Research Center, Moffett Field, California 94035 fI nu l nmIl l l l l

78 FR 65357 - Notice of Inventory Completion: University of Michigan, Ann Arbor, MI

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-31

... Hawaiian organizations, and has determined that there is no cultural affiliation between the human remains and associated funerary objects and any present-day Indian tribes or Native Hawaiian organizations. Representatives of any Indian tribe or Native Hawaiian organization not identified in this notice that wish to...

78 FR 65364 - Notice of Inventory Completion: University of Michigan, Ann Arbor, MI

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-31

... Hawaiian organizations, and has determined that there is no cultural affiliation between the human remains and associated funerary objects and any present-day Indian tribes or Native Hawaiian organizations. Representatives of any Indian tribe or Native Hawaiian organization not identified in this notice that wish to...

78 FR 65360 - Notice of Inventory Completion: University of Michigan, Ann Arbor, MI

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-31

... Hawaiian organizations, and has determined that there is no cultural affiliation between the human remains and associated funerary objects and any present-day Indian tribes or Native Hawaiian organizations. Representatives of any Indian tribe or Native Hawaiian organization not identified in this notice that wish to...

78 FR 65375 - Notice of Inventory Completion: University of Michigan, Ann Arbor, MI

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-31

... Hawaiian organizations, and has determined that there is no cultural affiliation between the human remains and associated funerary objects and any present-day Indian tribes or Native Hawaiian organizations. Representatives of any Indian tribe or Native Hawaiian organization not identified in this notice that wish to...

78 FR 65366 - Notice of Inventory Completion: University of Michigan, Ann Arbor, MI

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-31

... remains, in consultation with the appropriate Indian tribes or Native Hawaiian organizations, and has... or Native Hawaiian organizations. Representatives of any Indian tribe or Native Hawaiian organization..., transfer of control of the human remains to the Indian tribes or Native Hawaiian organizations stated in...

78 FR 65369 - Notice of Inventory Completion: University of Michigan, Ann Arbor, MI

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-31

... remains, in consultation with the appropriate Indian tribes or Native Hawaiian organizations, and has... or Native Hawaiian organizations. Representatives of any Indian tribe or Native Hawaiian organization..., transfer of control of the human remains to the Indian tribes or Native Hawaiian organizations stated in...

Language and Music as Communication: A Discussion.

ERIC Educational Resources Information Center

Brown, Roger; And Others

1979-01-01

Roger Brown, Diana Deutsch, Warren Benson, and Ruth Day comment on the similarities and differences between verbal language and music as forms of communication. This discussion occurred at the first session of the National Symposium on the Applications of Psychology to the Teaching and Learning of Music, Ann Arbor. (SJL)

Students Educating Each Other about Discrimination (SEED).

ERIC Educational Resources Information Center

Thornton, Miguel E.; And Others

This manual is for the Students Educating Each Other about Discrimination (SEED) program and describes its philosophy and curriculum. This curriculum was approved by the Ann Arbor (Michigan) Public Schools School Board. SEED tries to eliminate most forms of discrimination through a program in which high school students encourage sixth graders to…

40 CFR 80.93 - Individual baseline submission and approval.

Code of Federal Regulations, 2012 CFR

2012-07-01

... of its individual baseline to EPA (Fuel Studies and Standards Branch, Baseline Submission, U.S. EPA... Studies and Standards Branch, Baseline Petition, U.S. EPA, 2565 Plymouth Road, Ann Arbor, Michigan 48105..., used in the determination of a given fuel parameter; (iii) Identification of test method. If not per...

40 CFR 80.93 - Individual baseline submission and approval.

Code of Federal Regulations, 2013 CFR

2013-07-01

... of its individual baseline to EPA (Fuel Studies and Standards Branch, Baseline Submission, U.S. EPA... Studies and Standards Branch, Baseline Petition, U.S. EPA, 2565 Plymouth Road, Ann Arbor, Michigan 48105..., used in the determination of a given fuel parameter; (iii) Identification of test method. If not per...

40 CFR 80.93 - Individual baseline submission and approval.

Code of Federal Regulations, 2010 CFR

2010-07-01

... of its individual baseline to EPA (Fuel Studies and Standards Branch, Baseline Submission, U.S. EPA... Studies and Standards Branch, Baseline Petition, U.S. EPA, 2565 Plymouth Road, Ann Arbor, Michigan 48105..., used in the determination of a given fuel parameter; (iii) Identification of test method. If not per...

40 CFR 80.93 - Individual baseline submission and approval.

Code of Federal Regulations, 2011 CFR

2011-07-01

... of its individual baseline to EPA (Fuel Studies and Standards Branch, Baseline Submission, U.S. EPA... Studies and Standards Branch, Baseline Petition, U.S. EPA, 2565 Plymouth Road, Ann Arbor, Michigan 48105..., used in the determination of a given fuel parameter; (iii) Identification of test method. If not per...

Managerial Behavior: Developing Skills for Management Effectiveness in Times of Economic Setbacks

ERIC Educational Resources Information Center

Beatty, Richard W.; Morgan, Cyril P.

1975-01-01

The skills necessary for managerial success can be developed through methods directed at behavior change such as assessment centers, behaviorally anchored rating scales, role renegotiation, and feedback from educators. (Available from Office of Publications, Graduate School of Business Administration, University of Michigan, Ann Arbor, MI 48104,…

Homegrown Talent

ERIC Educational Resources Information Center

Masterson, Kathryn

2008-01-01

The University of Michigan at Ann Arbor is offering a development internship program that is designed to give students real-world experience working in development jobs and the chance to meet major donors and network with alumni. Its goals are lofty: to create a pipeline of young people for the development profession; diversify the fund-raising…

Group and Gender in Japanese and American Elementary Classrooms.

ERIC Educational Resources Information Center

Hamilton, V. Lee; And Others

1991-01-01

The individualism versus collectivism and androgynous versus gender-differentiated handling of children were studied in 10 Japanese (Chiba City) and 9 U.S. (Ann Arbor, Michigan) fifth grade classrooms with 407 and 246 students, respectively. Implications of the observed cultural variations in teacher behavior are discussed as they relate to…

Messages to Gail

ERIC Educational Resources Information Center

Selfe, Cindy, Ed.

2012-01-01

At the Computers and Writing 2011 Conference in Ann Arbor, Michigan, Gail E. Hawisher was celebrated for her many contributions to the field. At that conference, Hawisher gave a keynote address entitled "Our Work in the Profession: The Here and Now of the Future." This video publication includes contributions from scholars who wanted to share…

77 FR 45532 - Approval and Promulgation of Implementation Plans; Michigan; Detroit-Ann Arbor Nonattainment Area...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-01

... 2005 Base Year Emissions Inventory AGENCY: Environmental Protection Agency (EPA). ACTION: Proposed rule. SUMMARY: EPA is proposing to approve the fine particulate matter (PM 2.5 ) 2005 base year emissions...) plan, contingency measures, a 2005 base year emissions inventory and other planning SIP revisions...

Ablation Loss Studies for Capillary - Sustained Plasmas

DTIC Science & Technology

2006-11-01

forming network (PFN) power supply. The PFN power supply is used to charge a 1700-µF capacitor. The system-charging voltage ranged from 680 to 1210 V ...XAVIER BAGNOUD BLDG 1320 BEAL AVE ANN ARBOR MI 48109-2140 1 PA STATE UNIVERSITY DEPT OF MECHANICAL ENGINEERING T LITZINGER

40 CFR 59.213 - Incorporations by reference.

Code of Federal Regulations, 2014 CFR

2014-07-01

... available for inspection at the Air and Radiation Docket and Information Center, U.S. EPA, 401 M St., SW., Washington, DC 20460, the EPA Library (MD-35), U.S. EPA, Research Triangle Park, NC 27711, or at the National... University Microfilms International, 300 North Zeeb Road, Ann Arbor MI, 48106. (1) ASTM Method E220-86...

40 CFR 59.213 - Incorporations by reference.

Code of Federal Regulations, 2011 CFR

2011-07-01

... available for inspection at the Air and Radiation Docket and Information Center, U.S. EPA, 401 M St., SW., Washington, DC 20460, the EPA Library (MD-35), U.S. EPA, Research Triangle Park, NC 27711, or at the National... University Microfilms International, 300 North Zeeb Road, Ann Arbor MI, 48106. (1) ASTM Method E220-86...

40 CFR 59.213 - Incorporations by reference.

Code of Federal Regulations, 2012 CFR

2012-07-01

... available for inspection at the Air and Radiation Docket and Information Center, U.S. EPA, 401 M St., SW., Washington, DC 20460, the EPA Library (MD-35), U.S. EPA, Research Triangle Park, NC 27711, or at the National... University Microfilms International, 300 North Zeeb Road, Ann Arbor MI, 48106. (1) ASTM Method E220-86...

40 CFR 59.213 - Incorporations by reference.

Code of Federal Regulations, 2013 CFR

2013-07-01

... available for inspection at the Air and Radiation Docket and Information Center, U.S. EPA, 401 M St., SW., Washington, DC 20460, the EPA Library (MD-35), U.S. EPA, Research Triangle Park, NC 27711, or at the National... University Microfilms International, 300 North Zeeb Road, Ann Arbor MI, 48106. (1) ASTM Method E220-86...

40 CFR 72.13 - Incorporation by reference.

Code of Federal Regulations, 2010 CFR

2010-07-01

... and Material (ASTM), 1916 Race Street, Philadelphia, Pennsylvania 19103; and the University Microfilms International 300 North Zeeb Road, Ann Arbor, Michigan 48106. (1) ASTM D388-92, Standard Classification of Coals by Rank for § 72.2 of this chapter. (2) ASTM D396-90a, Standard Specification for Fuel Oils, for § 72...

An Investigation of the Memory Response of the Local Immune System to Shigella Antigens

DTIC Science & Technology

1989-03-03

CWM. Tugan NA: The histochemical demonstration of enterology 76:1035, 1979 protease by gelatin-silver film substance. J Histochem Cvtochem 23. Kotler DP...L. R. Armstrong , and J. E. Brown The Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA INTRODUCTION The

Cross-Dialectal Comparison: A Case Study and Some Theoretical Considerations

ERIC Educational Resources Information Center

Chen, Matthew

1973-01-01

Revised version of a paper delivered at the 5th International Conference on Sino-Tibetan Language and Linguistic Studies, Ann Arbor, Michigan, October 20-21, 1972. Assistance provided through a Summer Faculty Fellowship, University of California, San Diego, and the Phonology Laboratory at Berkeley (supported in part by a National Science…

How To Build It and Keep It Working.

ERIC Educational Resources Information Center

Crane, H. Richard

This book contains construction plans of exhibits that have been collected during more than 10 years, and notes for their maintenance, at the Ann Arbor (Michigan) Hands-On museum. It is a useful source of hard-to-find information for exhibit builders, science fair participants, science teachers, students, and home tinkerers. This collection…

Great River Environmental Action Team (GREAT II). Upper Mississippi River (Guttenberg, Iowa to Saverton, Missouri) Main Report.

DTIC Science & Technology

1980-12-01

followking studies: * Development studies that show the in- terrelated economic impacts (benefits and costs) that all industries located in tile The...Draitrac District. Anni Arbor Science, Ann Arbrr. Michiganr. Bowie. A. J.. C. C. Bohltot atrd J. A. Spraberr\\ . 19)75. Associated Genteral Contractors of

A North Korean Social Revolution in the Democratic People’s Republic of Korea

DTIC Science & Technology

2014-12-04

govern northern Korea. The DPRK regime began with Kim Il Sung and his North Korean Communist Party in the late Michigan: Pluto Press, 2008), 48, 79...Kwon. North Korea on the Brink: Struggle for Survival. Ann Arbor and London: Pluto Press, 2008. Fuqua Jr., Jacques L. Korean Unification: Inevitable

40 CFR 63.14 - Incorporations by reference.

Code of Federal Regulations, 2012 CFR

2012-07-01

...., Washington, DC, and at the EPA Library (MD-35), U.S. EPA, Research Triangle Park, North Carolina. For... (ASTM), 100 Barr Harbor Drive, Post Office Box C700, West Conshohocken, PA 19428-2959; or ProQuest, 300 North Zeeb Road, Ann Arbor, MI 48106. (1) ASTM D523-89, Standard Test Method for Specular Gloss, IBR...

78 FR 41993 - Ann Arbor Railroad, Inc.-Lease Exemption-Norfolk Southern Railway Company

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-12

... number of carloads interchanged with NSR in a given year.\\1\\ AARR states that the interchange commitment..., Canadian National Railway Company (CN), CSX Transportation, Inc., and Wheeling & Lake Erie Railway in Toledo; the Indiana and Ohio Railway and CN in Diann, Mich.; NSR in Milan, Mich.; and Great Lakes Central...

Extreme Makeover: IT Edition

ERIC Educational Resources Information Center

Schaffhauser, Dian

2011-01-01

Air-traffic controller might be a relaxing second career for anyone who's coordinated IT operations at a large research university. Just ask administrators at the University of Michigan in Ann Arbor. As at most big universities, IT operations on the academic side are decentralized on a major scale. When a faculty member in one of Michigan's 19…

40 CFR 52.1173 - Control strategy: Particulates.

Code of Federal Regulations, 2014 CFR

2014-07-01

... on the quantity of total dissolved solids in the quench water. (12) The State adopts and submits... establishes 2023 motor vehicle emissions budgets for the Detroit-Ann Arbor area of 4,360 tpy for primary PM2.5...), has been approved as submitted on July 5, 2011. The maintenance plan establishes 2023 motor vehicle...

Environmental Crisis: Root Causes and Opportunities for Solution.

ERIC Educational Resources Information Center

Horn, Barbara, Ed.

Proceedings of the 18th Annual Conference of the Conservation Education Association, held in Ann Arbor, Michigan, August, 1971, are reported in this booklet. Utilizing the theme - The Environmental Crisis: Root Causes and Opportunities for Solution - the program was aimed at environmental problems where we live; the urban area. It dealt with…

NCCA 2010 Water

EPA Pesticide Factsheets

Data from the National Aquatic Resource Surveys: The following data are available for download as comma separated values (.csv) files. Sort the table using the pull down menus or headers to more easily locate the data. Right click on the file name and select Save Link As to save the file to your computer. Make sure to also download the companion metadata file (.txt) for the list of field labels. See the survey technical document for more information on the data analyses.This dataset is associated with the following publications:Yurista , P., J. Kelly , and J. Scharold. Great Lakes nearshore-offshore: Distinct water quality regions. JOURNAL OF GREAT LAKES RESEARCH. International Association for Great Lakes Research, Ann Arbor, MI, USA, 42: 375-385, (2016).Kelly , J., P. Yurista , M. Starry, J. Scharold , W. Bartsch , and A. Cotter. The first US National Coastal Condition Assessment survey in the Great Lakes: Development of the GIS frame and exploration of spatial variation in nearshore water quality results. JOURNAL OF GREAT LAKES RESEARCH. International Association for Great Lakes Research, Ann Arbor, MI, USA, 41: 1060-1074, (2015).

Safety, immunogencity, and efficacy of a cold-adapted A/Ann Arbor/6/60 (H2N2) vaccine in mice and ferrets

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chen, Grace L., E-mail: chengra@niaid.nih.go; Lamirande, Elaine W., E-mail: elamirande@niaid.nih.go; Jin Hong, E-mail: jinh@medimmune.co

We studied the attenuation, immunogenicity and efficacy of the cold-adapted A/Ann Arbor/6/60 (AA ca) (H2N2) virus in mice and ferrets to evaluate its use in the event of an H2 influenza pandemic. The AA ca virus was restricted in replication in the respiratory tract of mice and ferrets. In mice, 2 doses of vaccine elicited a > 4-fold rise in hemagglutination-inhibition (HAI) titer and resulted in complete inhibition of viral replication following lethal homologous wild-type virus challenge. In ferrets, a single dose of the vaccine elicited a > 4-fold rise in HAI titer and conferred complete protection against homologous wild-typemore » virus challenge in the upper respiratory tract. In both mice and ferrets, the AA ca virus provided significant protection from challenge with heterologous H2 virus challenge in the respiratory tract. The AA ca vaccine is safe, immunogenic, and efficacious against homologous and heterologous challenge in mice and ferrets, supporting the evaluation of this vaccine in clinical trials.« less

77 FR 65196 - Announcement of the Award of a Single-Source Program Expansion Supplement Grant to the Regents of...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-25

... Child Abuse Prevention and Treatment and Adoption Reform Act (CAPTA) of 1978, (Pub. L. 95-266), as... DEPARTMENT OF HEALTH AND HUMAN SERVICES Administration for Children and Families [CFDA Number 93... the Board of the University of Michigan in Ann Arbor, MI AGENCY: Children's Bureau, Administration on...

Proceedings of the Annual Eastern Michigan University Conference on Languages for Business and the Professions (7th, Ann Arbor, Michigan, April 7-9, 1988).

ERIC Educational Resources Information Center

Des Harnais, Gaston, Ed.

Eighty-nine conference papers are presented in 10 sections: (1) language and cultural factors in conducting international business (qualifications for success as an international manager, staffing of international departments, role of second language proficiency, and international management concepts); (2) interdisciplinary language and business…

More Colleges Are Adding Family-Friendly Benefits

ERIC Educational Resources Information Center

Wilson, Robin

2008-01-01

Results of a new survey of family-friendly benefits by the Center for the Education of Women at the University of Michigan at Ann Arbor show that stopping the tenure clock has become the most common family-friendly benefit in higher education, following paid maternity leave. Other family-friendly policies that top the list in academe allow…

Preventing Dust Collection: Transforming Student Affairs Strategic Planning into Tangible Results

ERIC Educational Resources Information Center

Taylor, Simone Himbeault; Matney, Malinda M.

2007-01-01

The Division of Student Affairs at the University of Michigan in Ann Arbor engaged in an iterative strategic process to create and implement a set of long-range goals. This strategic journey continues to evolve, uniting a guiding framework of strategic planning steps, a reflective process with an assessment component within each step, and a group…

Current Campus Issues. Proceedings June 1969 Institutes on College and University Administration.

ERIC Educational Resources Information Center

University Consultants, Inc., Cambridge, MA.

The Institutes were held in Washington, D.C. and Ann Arbor, Mich. in June 1969, and were directed toward helping administrators develop greater senstivity and understanding of the critical issues facing them. This monograph contains a description of the 3-day "campus confrontation crisis" game played by the conference participants, and presents…

Micro-Accelerometers Monitor Equipment Health

NASA Technical Reports Server (NTRS)

2014-01-01

Glenn Research Center awarded SBIR funding to Ann Arbor, Michigan-based Evigia Systems to develop a miniaturized accelerometer to account for gravitational effects in space experiments. The company has gone on to implement the technology in its suite of prognostic sensors, which are used to monitor the integrity of industrial machinery. As a result, five employees have been hired.

ENVIRONMENTAL TECHNOLOGY VERIFICATION: JOINT (NSF-EPA) VERIFICATION STATEMENT AND REPORT HYDRO COMPLIANCE MANAGEMENT, INC. HYDRO-KLEEN FILTRATION SYSTEM, 03/07/WQPC-SWP, SEPTEMBER 2003

EPA Science Inventory

Verification testing of the Hydro-Kleen(TM) Filtration System, a catch-basin filter designed to reduce hydrocarbon, sediment, and metals contamination from surface water flows, was conducted at NSF International in Ann Arbor, Michigan. A Hydro-Kleen(TM) system was fitted into a ...

Summary Report of the Demonstration and Evaluation for the City/University EPSDT Day Care Project.

ERIC Educational Resources Information Center

Hierta, Ebba L., Ed.; Axelrod, Pearl G., Ed.

This summary report from a 3-year research and demonstration project describes the framework, methods and materials used by the University of Michigan-Ann Arbor Early Periodic Screening Diagnosis and Treatment (EPSDT) Day Care Project. The project served approximately 750 children and their families through day care centers and family day care…

Development of a Formal Theory of Agent-Based Computing for System Evaluation and System-Design Guidance

DTIC Science & Technology

2004-06-01

Department of EE and Computer Science University of Michigan Ann Arbor, MI 48109, USA pollackm@eecs.umich.edu Sujata Banerjeez Info. Sci. & Telecom. Dept...University of Pittsburgh Pittsburgh, PA 15260, USA sujata @tele.pitt.edu Abstract An important aspect of Business to Business E- Commerce is the agile

Automotive Design

NASA Technical Reports Server (NTRS)

1991-01-01

Analytical Design Service Corporation, Ann Arbor, MI, used NASTRAN (a NASA Structural Analysis program that analyzes a design and predicts how parts will perform) in tests of transmissions, engine cooling systems, internal engine parts, and body components. They also use it to design future automobiles. Analytical software can save millions by allowing computer simulated analysis of performance even before prototypes are built.

75 FR 37300 - Correction of Code of Federal Regulations: Removal of Temporary Listing of Benzylfentanyl and...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-29

... scheduled fentanyl compounds at the University of Michigan Medical School in Ann Arbor and at the Medical College of Virginia in Richmond. The studies indicated that while most of the fentanyl compounds had abuse... samples with other fentanyl analogues and were most likely unreacted intermediates in the synthesis of the...

Consultation-Conference on Developmental Disabilities and Gerontology. Proceedings of a Conference (Ann Arbor, Michigan, May 8-10, 1978).

ERIC Educational Resources Information Center

Segal, Robert M., Ed.

This document contains the proceedings from the National Conference on Developmental Disabilities and Gerontology, a conference designed to develop guidelines for program development on national, state, and local levels; develop and disseminate a proceedings publication; and provide an interface between professionals working at national, state,…

40 CFR 63.14 - Incorporations by reference.

Code of Federal Regulations, 2011 CFR

2011-07-01

... (MD-35), U.S. EPA, Research Triangle Park, North Carolina. For information on the availability of this..., Post Office Box C700, West Conshohocken, PA 19428-2959; or ProQuest, 300 North Zeeb Road, Ann Arbor, MI 48106. (1) ASTM D523-89, Standard Test Method for Specular Gloss, IBR approved for § 63.782. (2) ASTM...

GREAT LAKES REGIONAL ASSESSMENT: REPORT OF A WORKSHOP ON CLIMATE CHANGE IN THE UPPER GREAT LAKES REGION

EPA Science Inventory

The Upper Great Lakes workshop, sponsored by the U.S. Environmental Protection Agency (USEPA), was held at the University of Michigan in Ann Arbor, Michigan from 4-7 May 1998 to discuss some of the potential consequences of climate change in the Upper Great Lakes region (e.g., Mi...

Adapting the ADAM Manikin Technology for Injury Probability Assessment

DTIC Science & Technology

1992-02-19

Benson, J.B., Holstein , G.L., Melvin, J.W. Whole Body Response Research Program. Ann Arbor, MI, University of Michigan, Highway Safety Research...Liver Laceration I Fatal 25 227 9. UNKNOWN - COW ’ Aassiou" Ntoobor of 14my~ PAft Irni Type [ rid Ca ry Rados Neck tam 2nd D•s"* I Minimwl 4 Neck

World War I and the Attack of Professors of German and the University of Michigan.

ERIC Educational Resources Information Center

Wilcox, Clifford

1993-01-01

Describes social and intellectual climate at beginning of World War I that was exemplified by overzealous patriots demanding public displays of loyalty. Relates how six professors of German were permanently discharged by University of Ann Arbor. Describes incidents of goading by students and colleagues, and discusses failure of the American…

Annual Adult Education Research Conference. Proceedings (20th, Ann Arbor, Michigan, April 4-6, 1979).

ERIC Educational Resources Information Center

1979

Papers from numerous research areas in the adult education field are presented. The proceedings contain thirty-five papers, five symposia, one alternate symposium, and eighteen alternate papers. Among the papers included are "A Comparison of Approaches to Measuring Outcomes in Adult Basic Education,""A Critical Analysis of Hill's…

The Role of Model Fidelity in Model Predictive Control Based Hazard Avoidance in Unmanned Ground Vehicles Using Lidar Sensors

DTIC Science & Technology

2013-03-08

Mechanical Engineering University of Michigan Ann Arbor, MI 48109 ljch@umich.edu Paramsothy Jayakumar U.S. Army RDECOM-TARDEC Warren, MI 48397...5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) Jiechao Liu; Paramsothy Jayakumar ; James Overholt; Jeffrey Stein; Tulga Ersal 5d

Proceedings of the Annual Eastern Michigan University Conference on Languages for Business and the Professions (5th, Ann Arbor, Michigan, April 10-12, 1986).

ERIC Educational Resources Information Center

Voght, Geoffrey M., Comp.

Forty-five conference papers are presented in six sections: getting started in languages for special purposes (concerning teaching, curriculum development, finding, and resources); Spanish for business and the professions; French for business and the professions; other languages (English as a second language, German, Arabic, Mandarin Chinese, and…

Languages and Communication for World Business and the Professions. Proceedings of the Annual Conference (6th, Ann Arbor, Michigan, May 8-9, 1987).

ERIC Educational Resources Information Center

Des Harnais, Gaston R., Ed.

Topics covered in papers presented at a conference on languages and communication for world business and the professions include: (1) trends and aspects of internationalizing the business curriculum; (2) internationalized programs in business, foreign language, and cultures; (3) internationalized courses in business, foreign languages, and…

FY10 Defense Environmental Programs Annual Report to Congress

DTIC Science & Technology

2011-07-01

7,412- 2019  AIR FORCE ALPENA COUNTY REGIONAL AIRPORT / MI557282890300 17 2 $6,363 $0 $862 $1,9462012 2018  FORMERLY USED DEFENSE SITES ASH FIELD...Through FY10 ($000) IRP MMRPMMRPIRP Michigan ALPENA AAF / MI59799F231100 $001 $23 ALPENA NGTR / MI59799F228900 $610 $0 ANN ARBOR NGTR / MI59799F228800

Educator House Call: On-Line Data for Educators' Needs Assessment--Summary Report. NOAA Technical Memorandum GLERL-149

ERIC Educational Resources Information Center

Sturtevant, Rochelle A.; Marshall, Ann

2009-01-01

On July 15, 2009, National Oceanic and Atmospheric Administration's (NOAA's) Great Lakes Environmental Research Laboratory (GLERL) co-hosted a focus group--Educator House Calls: On-Line Data for Educators. The focus group was conducted at GLERL's main laboratory in Ann Arbor. The workshop was organized and funded by COSEE Great Lakes with student…

75 FR 3932 - Notice of Determinations Regarding Eligibility To Apply for Worker Adjustment Assistance

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-25

... Tube Company, Sharon, PA: July 22, 2009. TA-W-71,035; Prestige Printing, J.L. Wauford, Inc./Leased from...., Mitsubishi Motors North America, Technicon Int'l, Ann Arbor, MI: September 5, 2008. TA-W-72,468; EDAG, Inc.... TA-W-72,205; Charles Conkle Motor Company, Inc., Kokomo, IN. TA-W-72,231; Lonza Inc., Riverside...

Girls and Science and Technology. Proceedings and Contributions of the GASAT Conference (4th, Ann Arbor, Michigan, July 24-29, 1987).

ERIC Educational Resources Information Center

Daniels, Jane Zimmer, Ed.; Kahle, Jane Butler, Ed.

In 1979, North European researchers met informally to discuss issues regarding women in science. In addition to discovering that the issues raised crossed national boundaries and cultural differences, they found that numerous efforts were underway to address their concerns. What started as an informal meeting has evolved today into an…

Alternative Fuels Data Center: Michigan Fleet Reduces Gasoline and Diesel

Science.gov Websites

UseA> Michigan Fleet Reduces Gasoline and Diesel Use to someone by E-mail Share Alternative Fuels %. For information about this project, contact Ann Arbor Clean Cities Coalition. Download QuickTime Video Videos Photo of a car Hydrogen Powers Fuel Cell Vehicles in California Nov. 18, 2017 Photo of a car Smart

Proceedings of the Meeting of the Coastal Engineering Research Board (49th) Held in Oconomowoc, Wisconsin on 18-20 May 1988

DTIC Science & Technology

1989-02-01

Madsen , Neilsen, Madsen Dr. Guy A. Meadows, University of and Barber, Racine, Wisconsin Michigan, Ann Arbor, Michigan Mr. Donald H. McCreedy, Great Lakes...City. A recent study of this matter by real estate consultant Jared Schlaes indicates that a full 20 percent of the gross municipal product of

Access Denied: Mandatory Expulsion Requirements and the Erosion of Educational Opportunity in Michigan.

ERIC Educational Resources Information Center

Polakow-Suransky, Sasha

This report focuses on the impact of the Federal Gun-Free Schools Act (GFSA) in the Michigan public schools. The research for this study was conducted as a project of the Ann Arbor-based Student Advocacy Center. The study's purpose is twofold: (1) explore the implications of Michigan's zero-tolerance policy for educational equity and equal…

Proceedings of the Conference on Nutrition and Mental Retardation (Ann Arbor, Michigan, February 10-11, 1971).

ERIC Educational Resources Information Center

Springer, Ninfa Saturnino, Ed.

The conference, planned primarily for nutritionists and dieticians, dealt with the role of nutrition in the prevention and management of mental retardation. Proceedings include an overview of mental retardation, an examination of nutrition manpower needs in the fields of mental health and mental retardation on both the national and state levels,…

Evaluation of Minority Retention Programs: The Undergraduate Research Opportunities Program at the University of Michigan.

ERIC Educational Resources Information Center

Jonides, John; And Others

An evaluation was done of the first year of the Undergraduate Research Opportunities Program (UROP) at the University of Michigan (Ann Arbor), which is designed not only to teach students about research and/or certain academic topics, but also to facilitate the identification of minority students with the university. This second aim is based on…

Critical Language Awareness in the United States: Revisiting Issues and Revising Pedagogies in a Resegregated Society

ERIC Educational Resources Information Center

Alim, H. Samy

2005-01-01

As scholars examine the successes and failures of more than 50 years of court-ordered desegregation since "Brown v. Board of Education of Topeka, Kansas," and 25 years of language education of Black youth since "Martin Luther King Elementary School Children v. Ann Arbor School District Board," this article revisits the key…

Integrating Conservation Education Into the Existing Curriculum of the Ann Arbor Public School System (K-12).

ERIC Educational Resources Information Center

Stapp, William B.

The guiding principles incorporated into this program are: 1) to develop a kindergarten through twelfth grade curriculum in a logical sequence to provide continuity and progression in the program; 2) to have the program enhance the instructional goals of the school system; 3) to link the subject areas that relate most closely to conservation,…

A Michigan Faculty Group Plays Role of Watchdog

ERIC Educational Resources Information Center

Gose, Ben

2008-01-01

New administrative offices for diversity are popping up at universities around the country, and one can usually count on them to cast minority-recruiting efforts in a favorable light. There is still, however, a role for the old-school rabble-rousers, as a new report from a faculty committee at the University of Michigan at Ann Arbor makes clear.…

Drop/Gas Interactions in Dense Sprays

DTIC Science & Technology

2001-06-19

Xavier Bagnoud Bldg. Ann Arbor, Michigan 48109-2140, U.S.A. SUMMARY/OVERVIEW: Turbulence generation and liquid breakup are being studied due to their...S10-2 10-2 I E-1 1 E- ’ V 10 1-3C 1 E-3 _________ CHEN&FAETH(1 999) PREDICTIONS d,.0.5rmm dp (mm) SYM. n",,(kpartshims) SYM. 0.5------------ IA- 12

9. international mouse genome conference

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

This conference was held November 12--16, 1995 in Ann Arbor, Michigan. The purpose of this conference was to provide a multidisciplinary forum for exchange of state-of-the-art information on genetic mapping in mice. This report contains abstracts of presentations, focusing on the following areas: mutation identification; comparative mapping; informatics and complex traits; mutagenesis; gene identification and new technology; and genetic and physical mapping.

Anthropometry: Basic Studies ad Applications. Volume 1. 1964-1975

DTIC Science & Technology

1977-09-01

Human factors engineering. Cadavers, Moments of inertia, Mass, Aerospace medicine, Tables(Data), Responses IDENTIFIERS: DOT/5A, NTlSDOTHTS PB-241...muscles; head/neck response to low-level acceleration, voluntary isometric muscle force in the lateral direction; and three-dimensional range of motion...Prices: PC$7.50/MFS2.25 \\ 77 22 Whole Body Response Research Program Michigan Univ., Ann Arbor. Dept* of Biomechanics.*General Motors

Dollar Summary of Prime Contract Awards by State, Place, and Contractor, FY84, Part 3, (Anderson, Indiana-Winona, Mississippi).

DTIC Science & Technology

1984-01-01

ALMONT ALMONT WELDING WORKS INC 102 102 ALPENA FLETCHER MOTEL 27 27 HARRYS OIL CO 65 65 HOMANT OIL CO 117 117 KOSS INDUSTRIES INC 538 538 PARSONS G S...COMPANY 108 Joe XEROX CORPORATION 59 59 ZOLNIEREK DAVID J INC 43 43 TOTAL - ALPENA 957 667 290 ANN ARBOR ADVERTEL COMMUNICATION INC 60 AMAX MATERIALS

Air Force Research Laboratory High Power Electric Propulsion Technology Development

DTIC Science & Technology

2009-10-27

Plasmas in a Coaxial Double Theta Pinch, “ Doctoral Dissertation, Department of Aerospace Engineering, University of Michigan, Ann Arbor, MI, 2008. [6...surpasses the level of DARPA FAST goals. Several evolving propulsion concepts may enable a viable high-power plasma propulsion device suitable for...of PEPL) 5 performance operation with multiple cathodes or in a single- shared cathode configuration [4]. However, the local plasma properties

Conflict over Relocation Divides a Catholic Law School

ERIC Educational Resources Information Center

Mangan, Katherine

2007-01-01

A mutiny may be brewing at a Catholic law school whose board has voted to pack it up and move it from Ann Arbor, Michigan to a rural community in southwest Florida. More than half of the professors at Ave Maria School of Law are fighting the move to Ave Maria, Florida, the town being created by Thomas S. Monaghan, the Domino's Pizza mogul who…

Evaluation of Motor Gasoline Stability

DTIC Science & Technology

1990-12-01

CAMPINNE AIR POLLUTION CONTROL I LABORATOIRE MECANIQUE TRANSPORT 2565 PLYMOUTH ROAD AVENUE DE LA RENAISSANCE, 3D ANN ARBOR MI 48105 B-1040 BRUSSELS 5...CUARTEL GENERAL DEL EJERCITO ATTN: MR K LAURINSEN ATTN: MAJ M ENGO NOGUES GADHOLTVEJ 11 DIVISION DE LOGISTICA (DIAM/LABCAMVE) DK-9900 FREDERIKSHAVN...Gum for Typical Civilian Gasolines Transported by CEPS and F-46 Gasolines Stored by CEPS ...................................... 47 B. Potential Gum

Improving Self Worth by Learning to Cope with Distress: A Teaching Model Produced for Mid-Adolescents in the Ann Arbor Public Schools.

ERIC Educational Resources Information Center

Love, James G.

The goal of this teaching model, which is designed to occupy approximately 8 class periods of 50 minutes each, is to improve the health and well-being of high school students through instruction in recognizing personal distress and utilizing effective coping techniques. Each of the six lessons (Introduction, Recognizing Our Stress Symptoms, How…

Campus Planning 1967. Selected Papers from the Annual Conference of the Society for College and University Planning (2nd, Ann Arbor, Michigan, August 20-22, 1967).

ERIC Educational Resources Information Center

Mayer, Frederick W., Ed.; Schmult, Carl V. Jr., Ed.

The annual conference encouraged communication among individuals and disciplines relating to long-range development of institutions of higher education. Ideas, comments, and major speeches were assembled and published. Major areas of interest following the introduction are--(1) college and university planning in Canada, (2) the educational…

Proceedings of the Annual Midwest Research-to-Practice Conference in Adult and Continuing Education (4th, Ann Arbor, Michigan, October 10-11, 1985).

ERIC Educational Resources Information Center

Berlin, L. S., Ed.

This document contains the following papers on practical applications of research on adult and continuing education: "Elderly Criminal Behavior: Linking Research to Practice," by Donald J. Bachand and Carl I. Brahce; "Father? Teacher? Friend? Instructor-Student Relationships in a Refugee Class," by Gary J. Bekker; "The Small Group: Understanding…

Interferometry of Epsilon Aurigae: Characterization of the Asymmetric Eclipsing Disk

DTIC Science & Technology

2015-09-21

Observatory, Mount Wilson, California 91023, USA; bkloppenborg@chara.gsu.edu 2 Department of Physics & Astronomy, University of Denver, Denver, CO...80208 USA 3 Department of Astronomy, University of Michigan, 941 Dennison Building, Ann Arbor, Michigan 48109, USA 4 Department of Physics , Central...Observatory Road, Flagstaff, Arizona 86001, USA 6 Department of Physics and Astronomy, Penn. State, University Park, PA 16802 7 School of Engineering

The Zapatista Social Netwar in Mexico

DTIC Science & Technology

1998-01-01

on the Internet. 4From a statement by Subcommandante Marcos, March 4, 1994, as reported by the Academia Mexicana de Derechos Humanos , Special...Indigenas de Mexico, Mexico City: Comisiön Nacional de Derechos Humanos , 1995. Evan, William M., "An Organization-Set Model of Interorganizational...City: Comision Nacional de Dere- chos Humanos , 1995. Meisel, James, The Fall of the Republic: Military Revolt in France, Ann Arbor: University of

Identification of an Adaptable Computer Program Design for Analyzing a Modular Organizational Assessment Instrument.

DTIC Science & Technology

1981-09-01

ber) Survey-guided development Organizational effectiveness Computer program Organizational diagnosis Management 20. ABSTRACT (Continue an reverse...Army. Doctoral dissertation, Purdue University, December 1977. (DTIC AD-A059-542) Bowers, D. G. Organizational diagnosis : A review and a proposed method...G. E. Compara- tive issues and methods in organizational diagnosis . Ann Arbor MI: Institute for Social Research, University of Michigan, November 1977

Visual Form Detection in 3-Dimensional Space.

DTIC Science & Technology

1982-10-01

RR04209 Ann Arbor, Michigan 48109 RR0429002; NR 197-070 - II CONTROLLING OFFICE NAME AND ADDRESS 12. REPORT DATE Engineering Psychology Group ( Code...93940 Pasadena, CA 91106 Dean of Research Administration Office of Naval Research Naval Postgraduate School Scientific Liaison Group Monterey, CA...Eisenhower Avenue Dr. Gloria Chisum Alexandria, VA 22333 Sciences Research Group Code 6003 Naval Air Development Center Warminste.’, PA 18974 -4- Department

Sound Modeling Simplifies Vehicle Noise Management

NASA Technical Reports Server (NTRS)

2015-01-01

Under two SBIR contracts with Langley Research Center, Ann Arbor, Michigan-based Comet Technology Corporation developed Comet EnFlow, a software program capable of predicting both high- and low-frequency noise and vibration behavior in plane fuselages and other structures. The company now markets the software to airplane, automobile, and ship manufacturers, and Langley has found an unexpected use for it in leak detection on the International Space Station.

Editorial Conference Comments by the Editors

NASA Astrophysics Data System (ADS)

Fabris, Lorenzo; Valentine, John D.; Barton, Paul; Derenzo, Stephen; Archer, Daniel E.; Bell, Zane W.; Brubaker, Erik; Conway, Adam M.; Dalla Betta, Gian-Franco; De Geronimo, Gianluigi; Fiorini, Carlo; Guazzoni, Chiara; Hayward, Jason P.; Kernan, Warnick J.; Labov, Simon E.; Payne, Stephen; Re, Valerio; Rozenfeld, Anatoly; Runkle, Robert; Sturm, Benjamin; Yang, Liang; Zhuravleva, Mariya; Ziock, Klaus-Peter

2017-07-01

The Symposium on Radiation Measurements and Applications (SORMA) convened for the third time on the West Coast, May 22-26, 2016, at the Clark Kerr Campus of the University of California, Berkeley, CA, USA. With radiation detectors increasing in number, variety, and societal importance, we are alternating between SORMA (in Ann Arbor, MI, USA) and SORMA West so that the forum will be available every two years.

[Change of Plasma Interleukin-17 Level in Patients with Extranodal NK/T-Cell Lymphoma and Its Clinical Significance].

PubMed

Shang, Chun-Xiang; Ma, Ji-Cheng; Nan, Zheng; Li, Ye; He, Wen-Cai; Pan, Xian-Ying

2017-06-01

To investigate the clinical significance of interleukin-17 (IL-17) level in patients with extranodal NK/T-cell lymphoma(ENKTL). Eighty patients with nasal ENKTL who received radiotherapy, chemotherapy or radiotherapy combined with chemotherapy from January 2011 to January 2012 were enrolled in the study. Eighty healthy volunteers were selected as the controls (control group). About 5 ml of peripheral blood was collected from all patients and controls. IL-17 level was determined by ELISA. The age, sex, ECOG score, B symptoms, LDH level, lymph node involvment, Ann Arbor stage, IPI, KPI, peripheral blood lymphocyte and lymph node metastasis, number of lymphocytes and monocytes in peripheral blood were recorded. All patients were followed up for 3 year progression-free survival (PFS) and overall survival (OS). The average IL-17 level in patients with ENKTL was 6.48 pg/ml and the average concentration of IL-17 in control group was 0.56 pg/ml (P<0.01). The level of IL-17 in patients with B-symptoms and lymph node involvement was significantly higher than that in the control group. The differences in IL-17 level were not statistically significant among patients with different age, sex, ECOG, LDH, Ann Arbor stage, IPI, KPI, lymphocyte count and monocyte cell count. The sensitivity and specificity of IL-17 were 74.5% and 73.7% respectively, and the optimal threshold was 3.49 pg/ml and AUC was 0.799 (95% CI: 0.688-0.909) (P<0.01). The PFS and OS were longer in the patients with IL-17≤3.49 pg/ml and longer in the patients without lymph node involvement and Ann Arbor I. Multivariate analysis showed that independent predictors of PFS and OS in patients with ENKTL were plasma IL-17 levels and age (P<0.05). ENKTL patients with different clinical characteristics have different levels of IL-17, the different level of IL-17 has different effects on prognosis of patients with ENKTL.

Hands-on physics displays for undergraduates

NASA Astrophysics Data System (ADS)

Akerlof, Carl W.

2014-07-01

Initiated by Frank Oppenheimer in 1969, the Exploratorium in San Francisco has been the model for hands-on science museums throughout the world. The key idea has been to bring people with all levels of scientific background in contact with interesting and attractive exhibits that require the active participation of the visitor. Unfortunately, many science museums are now forced to cater primarily to very young audiences, often 8 years old or less, with predictable constraints on the intellectual depth of their exhibits. To counter this trend, the author has constructed several hands-on displays for the University of Michigan Physics Department that demonstrate: (1) magnetic levitation of pyrolytic graphite, (2) the varied magnetic induction effects in aluminum, copper and air, (3) chaotic motion of a double pendulum, (4) conservation of energy and momentum in a steel ball magnetic accelerator, (5) the diffraction pattern of red and green laser pointer beams created by CDs and DVDs, (6) a magnetic analog of the refraction of light at a dielectric boundary and (7) optical rotation of light in an aqueous fructose solution. Each of these exhibits can be constructed for something like $1000 or less and are robust enough to withstand unsupervised public use. The dynamic behavior of these exhibits will be shown in accompanying video sequences. The following story has a history that goes back quite a few years. In the late 70's, I was spending time at the Stanford Linear Accelerator Center accompanied by my family that included our two grade school children. Needless to say, we much enjoyed weekend excursions to all sorts of interesting sites in the Bay Area, especially the Exploratorium, an unusual science museum created by Frank Oppenheimer that opened in 1969. The notion that exhibits would be designed specifically for "hands-on" interactions was at that time quite revolutionary. This idea captivated a number of people everywhere including a friend in Ann Arbor, Cynthia Yao. With a core group of a few dozen people, Cynthia convinced the City of Ann Arbor to allow free use of an old firehouse. The Ann Arbor Hands-On Museum opened its doors to the public in 1982 and remains a thriving institution to this day.

18F-FDG PET/CT in gastric MALT lymphoma: a bicentric experience.

PubMed

Albano, Domenico; Bertoli, Mattia; Ferro, Paola; Fallanca, Federico; Gianolli, Luigi; Picchio, Maria; Giubbini, Raffaele; Bertagna, Francesco

2017-04-01

The role of 18F-FDG-PET/CT in evaluating gastric MALT lymphoma is still controversial. In the literature the detection rate of 18F-FDG-PET/CT in patients with gastric MALT lymphoma is variable, and the reason for this heterogeneity is not still clear. Our aim was to investigate the particular metabolic behavior of these lymphoma. Sixty-nine patients (26 female, 43 male) with histologically confirmed gastric MALT lymphoma who underwent a 18F-FDG-PET/CT for initial staging from two centers were included. The PET images were analyzed visually and semi-quantitatively by measuring the maximum standardized uptake value (SUVmax), lesion-to-liver SUVmax ratio, and lesion-to-blood pool SUVmax ratio and compared with Ann Arbor stage, epidemiological (age, sex), histological (presence of gastritis, ulcer, H. pylori infection, plasmacytic differentiation, Ki-67 index), and morphological (tumor size, superficial lesions or mass-forming) characteristics. Thirty-six patients (52 %) had positive PET/CT (average SUVmax was 9±6.7; lesion-to-liver SUVmax ratio 3.7±2.6, lesion-to-blood pool SUVmax ratio 4.8±3.3) at the corresponding gastric lesion; the remaining 33 were not 18F-FDG-avid. In the univariate analysis, 18F-FDG avidity was significantly associated with morphological features (mass forming p<0.001 and high maximum diameter p<0.001), Ann Arbor stage (p=0.010), and Ki67 index (p<0.001) and not correlated with age, sex, presence of gastritis, ulcer, Helicobacter pylori infection, and plasmacytic differentiation. In the multivariate analysis, the correlations with gross morphological appearance, Ann Arbor stage, and Ki-67 score were confirmed. SUVmax, lesion-to-liver SUVmax ratio, and lesion-to-blood pool SUVmax ratio correlated significantly only with Ki67 index (p=0.047; p=0.012; p=0.042). 18F-FDG avidity was noted in 52 % of gastric MALT lymphoma and this avidity is correlated with gross morphological characteristics, tumor stage, and Ki-67 index. SUVmax, lesion-to-liver SUVmax ratio, and lesion-to-blood pool SUVmax ratio are correlated only with Ki-67 index, and only lesion-to-liver SUVmax ratio was independently associated with Ki-67 score.

A Wireless Internet-Based Observatory: The Real-time Coastal Observation Network (ReCON)

DTIC Science & Technology

2007-09-01

48105 J. J. Gray National Oceanic and Atmospheric Administration Thunder Bay National Marine Sanctuary 500 W. Fletcher St. Alpena , MI 49707 S...underwater hubs with sensors. Shore Station Buoy or Permanent Station Alpena Muskegon Milwaukee Ann Arbor Chicago Cleveland Toledo...meteorological station located on TBI, and a shore station located at the Maritime Heritage Center in Alpena , MI. A digital video camera will be connected to

The Roles of the Bone Marrow Microenvironment in Controlling Tumor Dormancy

DTIC Science & Technology

2015-10-01

Shiozawa, M.D., Ph.D. CONTRACTING ORGANIZATION: Wake Forest School of Medicine Ann Arbor, MI 48109 REPORT DATE: October 2015 TYPE OF REPORT...PERFORMING ORGANIZATION REPORT NUMBER Wake Forest University Health Sciences Medical Center Blvd. Winston-Salem NC 27157 9. SPONSORING... Wake Forest School of Medicine as of 03/01/15. The PI obtained the necessary institutional approvals (IACUC, IRB, IBC) and submitted the grant

"H TEXNH: Research Methods and Topics for the History of Rhetoric"; Proceedings of the Speech Communications Association Doctoral Honors Seminar (Ann Arbor, Michigan, March 3-5, 1978).

ERIC Educational Resources Information Center

Enos, Richard Leo, Ed.; Wiethoff, William E., Ed.

The interpretation of the history of rhetoric was the subject of the seminar reported in this document. After a preface and a discussion of the "promise" of rhetoric, abstracts of seminar presentations are grouped under the headings of rhetoric and culture, rhetoric and philosophy, methodological considerations, and discourse analysis.…

Composition-spread Growth and the Robust Topological Surface State of Kondo Insulator SmB6 Thin Films

DTIC Science & Technology

2014-01-01

1,2 1 Center for Nanophysics & Advanced Materials , University of Maryland, College Park, Maryland 20742, USA 2 Department of physics, University of...Maryland, College Park, Maryland 20742, USA 3 Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC 27708 4...Department of Materials Science and Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA 5 Department of Materials Science & Engineering

Improving Government Procurement: Lessons from the Coast Guard’s Deepwater Program

DTIC Science & Technology

2010-09-17

dilemma. Ann Arbor, MI: University of Michigan Press. Mankiw , G. (2002). Macroeconomics (5th ed.). New York, NY: Worth. Martin, L. L. (2004...Documentation Page Form ApprovedOMB No. 0704-0188 Public reporting burden for the collection of information is estimated to average 1 hour per response...the collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including

Optical Near-Field Plates

DTIC Science & Technology

2015-04-08

AFRL-OSR-VA-TR-2015-0085 OPTICAL NEAR-FILED PLATES Roberto Merlin UNIVERSITY OF MICHIGAN Final Report 04/08/2015 DISTRIBUTION A: Distribution...03-2015 Final 09/01/2009-12/31/2014 Optical Near-Field Plates FA9550-09-1-0636 erlin, Roberto, D. The University of Michigan Ann Arbor, MI 48109...distribution unlimited Optical near-field plates were designed using antisymmetric plasmon modes to generate abrupt phase changes within a fraction of a

Alternatives to Address Role Clarification at the U.S. Army Community Hospital, Fort Polk

DTIC Science & Technology

1981-06-01

Szilagyi , Andrew D., and Wallace , Marc J., Organizational Behavior and Performance (Santa Monica: Goodyear Publishing Company, 1977): Page 214. 14...Institute For Social Research, The University of Michigan, Ann Arbor, Michigan, 1972. Ivancevich, John M., Szilagyi , Andrew D. and Wallace , Marc J...opposed to professional and technical groups. 1 1 Another study by Szilagyi and Sims postulated the existence of a role ambiguity continuum which revealed

Commentary on the Future of Community Psychology: Perspective of a Research Community Psychologist.

PubMed

Milburn, Norweeta G

2016-12-01

Community psychology is commented upon from the perspective of a community psychologist who was trained in the Community Psychology Program at the University of Michigan in Ann Arbor. Her background and training are reviewed. A brief survey of research on homelessness as a frame for community psychology research is presented. Concluding remarks are provided on the future of research in community psychology. © Society for Community Research and Action 2016.

The Freshman Year in Science and Engineering: Old Problems, New Perspectives for Research Universities. Report of a Conference (Ann Arbor, Michigan, April 6-7, 1990).

ERIC Educational Resources Information Center

Wineke, William R.; Certain, Phillip

The goal of the conference reported in this document was to initiate major revitalization of freshman science by bringing together individuals who have been working to improve introductory courses with research faculty who may or may not have been actively involved in the teaching of these courses. This report tries to capture the spirit and the…

Hypertonic/Hyperoncotic Resuscitation from Shock: Reduced Volume Requirement and Lower Intracranial Pressure

DTIC Science & Technology

1989-10-01

in Dogs with Hemorrhagic Shock and an Intracranial Mass. Seventh International Symposium on Intracranial Pressure and Brain Injury , Ann Arbor, Michigan...with Hemorrhagic Shock and an Intracranial Mass. Seventh International Symposium on Intracranial Pressure and Brain Injury . Intracranial Pressure VII...and MCI US groups. Discussion: Following this severe insult a iETTiFMT3-a clinical head injury combined wit6i hemorrha Ic shock, a cobntnc/h rcctc

United States Military Retirement Migration: Patterns and Processes.

DTIC Science & Technology

1984-03-27

active duty force e) Number of heating degree days tA 23 f) Number of physicians per 1000 population g) Recreation acres per 1000 population h...Eva Mueller. 1967. The Geographic Mobility of Labor. Ann Arbor : University of Michigan. Institute for Social Research, Survey Research Center. Law, C.M... Chicken or Egg?. Southern Economic Journal. 37:295-306. Muth, J.F. 1961. Rational Expectations and the Theory of Price Movements. Econometrics. 29:315

The Growth Edge: Creative Use of Computers for Facilitating Learning and Enhancing Personal Development. Papers from the Workshop (Ann Arbor, Michigan, June 27-30, 1986).

ERIC Educational Resources Information Center

Walz, Garry R., Ed.; Bleuer, Jeanne C., Ed.

This document is the fourth publication in a series devoted to the use of computers in counseling. The outgrowth of the 1986 ERIC/CAPS workshop, it contains four of the major presentations made at the conference. "The Impact of Computers on the Future of Counseling: Boom or Boomerang" (Edwin L. Herr) examines the effect of technology…

Technical Report for Contract N00039-82-C-0235, 15 November 1981-30 September 1983

DTIC Science & Technology

1983-09-30

Management of Data, Ann Arbor, Rich., June 1982. -. 2 ’’.’ , . .. ’,, .* ,° • ,"%,.° % °%" ° %- " "%" . . ." " -.. . . ,.%. Interactive Mathematical ...developed and implemented a hierachical representation for mathematical expres- sioms that includes display position, expression dimensions, font...etc) in internal forms are accepted and converted to box frames which can be displayed. 2. Strophe’s representation of mathematical expressions is

A New Family of Models for the Multiple-Choice Item.

DTIC Science & Technology

1979-12-19

analysis of the verbal scholastic aptitude test using Birnhaum’s three-parameter logistic model. Educational and Psychological Measurement, 28, 989-1020...16. [8] McBride, J. R. Some properties of a Bayesian adaptive ability testing strategy. Applied Psychological Measurement, 1, 121-140, 1977. [9...University of Michigan Ann Arbor, MI 48106 ’~KL -137- Non Govt Mon Govt 1 Dr. Earl Hunt 1 Dr. Frederick N. Lord Dept. of Psychology Educational Testing

Mindfulness & Self-Compassion Meditation for Combat Posttraumatic Stress Disorder: Randomized Controlled Trial and Mechanistic Study

DTIC Science & Technology

2013-10-01

VA Ann Arbor PTSD clinic; and c.) conducting a translational neuroimaging mechanistic study with pre- and post fMRI and neurocognitive testing . 15...might be helpful – both in terms of the psychological characteristics of change, and in terms of neural mechanisms in the brain. Mindfulness...neurocognitive testing . Our novel 16 week Mindfulness and Self-compassion group intervention, “Mindfulness-based Exposure therapy” (MBET), was developed

A live attenuated H7N7 candidate vaccine virus induces neutralizing antibody that confers protection from challenge in mice, ferrets and monkeys

USDA-ARS?s Scientific Manuscript database

A live attenuated H7N7 candidate vaccine virus was generated by reverse genetics using the modified hemagglutinin (HA) and neuraminidase (NA) genes of HP A/Netherlands/219/03 (NL/03) (H7N7) wild-type (wt) virus and the six internal protein genes of the cold-adapted (ca) A/Ann Arbor/6/60 ca (AA ca) (...

Diesel Engine Technology Update

DTIC Science & Technology

1987-07-01

AFWAL-TR-87-20 54 83-021-DET DIESEL ENGINE TECHNOLOGY UPDATE Kaupert, Andrew W., Lt. Col. USAFR Air Force Reserves Detroit Detachment 2 Ann Arbor, MI...sponsored adiabatic turbocompound diesel engine . One goal was the use of no water or air cooling for the engine to enable the minimized heat transfer from...sector with severe • impact on the stationary engine segment of the marketplace. The effect of this proposed legislation on Air Force fuel quality is

TREC 2014 Web Track Overview

DTIC Science & Technology

2015-02-18

S) 5d. PROJECT NUMBER 5e. TASK NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) University of Michigan,Ann Arbor,MI...conference was co-sponsored by the National Institute of Standards and Technology (NIST) and the Defense Advanced Research Projects Agency (DARPA). 14...and B corpora were created may be found on the Lemur project website2. We strongly encouraged participants to use the full Category A data set if

The Detection of Faint Space Objects Using Solid State Imaging Detectors.

DTIC Science & Technology

1983-12-31

are con.iposed of baryonic matter . New arguments were presented against halos being composed of planets or asteroids. D. Hegyi was also invited to...being made up of baryonic matter . 5.0 THE CHARGE-COUPLED DEVICE IMAGING SYSTEM Our major hardware improvement during the past year is a stainless steel...Hegyi Department of Physics University of Michigan Ann Arbor, Michigan ABSIR:CT The problems with massive halos being composed of baryonic matter are

The Prevalence of Concha Bullosa and Nasal Septal Deviation and Their Relationship to Maxillary Sinusitis by Volumetric Tomography

DTIC Science & Technology

2010-07-01

2Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI 48109, USA 3Lieutenant Colonel, US Army DENTAC...of the region, independently reviewed Report Documentation Page Form ApprovedOMB No. 0704-0188 Public reporting burden for the collection of...Operations and Reports , 1215 Jefferson Davis Highway, Suite 1204, Arlington VA 22202-4302. Respondents should be aware that notwithstanding any other

Subspace Methods for Massive and Messy Data

DTIC Science & Technology

2017-07-12

Subspace Methods for Massive and Messy Data The views, opinions and/or findings contained in this report are those of the author(s) and should not...AGENCY NAME(S) AND ADDRESS (ES) U.S. Army Research Office P.O. Box 12211 Research Triangle Park, NC 27709-2211 REPORT DOCUMENTATION PAGE 11. SPONSOR...Number: W911NF-14-1-0634 Organization: University of Michigan - Ann Arbor Title: Subspace Methods for Massive and Messy Data Report Term: 0-Other

Target Surface Area Effects on Hot Electron Dynamics from High Intensity Laser-Plasma Interactions

DTIC Science & Technology

2016-08-19

New J. Phys. 18 (2016) 063020 doi:10.1088/1367-2630/18/6/063020 PAPER Target surface area effects on hot electron dynamics from high intensity laser ...Science, University ofMichigan, AnnArbor,MI 48109-2099, USA E-mail: czulick@umich.edu Keywords: laser -plasma,mass-limited, fast electrons, sheath...field Abstract Reduced surface area targets were studied using an ultra-high intensity femtosecond laser in order to determine the effect of electron

Flexural and Tensile Properties of Thin, Very High-Strength, Fiber-Reinforced Concrete Panels

DTIC Science & Technology

2008-09-01

Fiber - Reinforced Concrete Panels Michael J. Roth September 2008 G eo te ch ni ca l a nd S tr uc tu re s La bo ra to ry Approved for...Tensile Properties of Thin, Very High-Strength, Fiber - Reinforced Concrete Panels Michael J. Roth Geotechnical and Structures Laboratory U.S. Army...of Michigan, Ann Arbor, and noted authority in the field of 160 fiber - reinforced concrete . Implementation of Li’s work

Measured Properties of Turbulent Premixed Flames for Model Assessment, Including Burning Velocities, Stretch Rates, and Surface Densities (Postprint)

DTIC Science & Technology

2006-10-01

Engineering) Campbell D. Carter and Jeffrey M. Donbar (Aerospace Propulsion Division, Propulsion Sciences Branch (AFRL/ PRAS )) 5f. WORK UNIT NUMBER...Sciences Branch (AFRL/ PRAS ) Propulsion Directorate Air Force Research Laboratory, Air Force Materiel Command Wright-Patterson AFB, OH 45433-7251 Uni. of...M. Donbar b a Department of Aerospace Engineering, University of Michigan, Ann Arbor, MI 48109, USA b Air Force Research Laboratory AFRL/ PRAS , Wright

Integration of Lower Level Supervisors into the Management Structure.

DTIC Science & Technology

1981-08-31

sessions. Brief (2-3 hour) training "work sessions" could be developed to help lower level supervisors 4 solve specific problema . These sessions...34 In D. Cartwright (ed.), Studies in Social Power. Ann Arbor, MI: The University of Michigan Press, 1959. Gardner, B.B. & Whyte, W.F. "The man in the...line supervisory problem redefined." Personnel Journal, 1975, 54(12), 620-623+. Stouffer, S.A. "An analysis of conflicting social norms." American

International Conference: Paraoxonases - Basic and Clinical Directions of Current Research (1st) Held in Ann Arbor, Michigan on April 22-24, 2004

DTIC Science & Technology

2005-04-01

cholesterol lowering by simvastatin and atorvastatin Mark Roest, UMC Utrecht, Utrecht, The Netherlands Population-based and family studies suggest an...lowering by simvastatin and atorvastatin * Mark ROEST, UMC Utrecht, Utrecht, The Netherlands 20. Study of factors influencing the decreased paraoxonase...lowering by simvastatin and atorvastatin . Mark Roest1 , Thomas van Himbergen1, 2, Jacqueline de Graaf 2, Hiroaki Hattori 3, John Kastelein4

Adaptive Piezoelectric Circuitry Sensor Network with High-Frequency Harmonics Interrogation for Structural Damage Detection

DTIC Science & Technology

2014-09-17

AFRL-OSR-VA-TR-2014-0255 ADAPTIVE PIEZOELECTRIC CIRCUITRY SENSOR NETWORK KON -WELL WANG MICHIGAN UNIV ANN ARBOR Final Report 09/17/2014 DISTRIBUTION A...Harmonics Interrogation for Structural Damage Detection FA9550-11-1-0072 Kon -Well Wang and Jiong Tang The Regents of the University of Michigan, 3003...mechanism. These efforts have yielded a complete methodology of adaptive high-frequency piezoelectric self-sensing interrogation. None None None SAR Kon

NOTES FOR COMMUNITY COLLEGE TRUSTEES, I, II--REPORTS OF ANNUAL CONFERENCES CONDUCTED FOR COMMUNITY COLLEGE TRUSTEES AND PRESIDENTS BY THE MIDWEST COMMUNITY COLLEGE LEADERSHIP PROGRAM (1ST, 2D, 3D, ANN ARBOR, MICHIGAN).

ERIC Educational Resources Information Center

BANFIELD, RALPH W.; HALL, GEORGE L.

PAPERS PRESENTED AT THREE CONFERENCES FOR COMMUNITY COLLEGE TRUSTEES AND PRESIDENTS INCLUDED (1) A CONSIDERATION OF THE ROLES OF THE LIBERAL ARTS COLLEGE, THE UNIVERSITY, AND THE COMMUNITY COLLEGE, (2) FACULTY RELATIONS WITH ADMINISTRATION AND STAFF, (3) A CASE STUDY OF THE DEVELOPMENT OF THE ST. LOUIS JUNIOR COLLEGE DISTRICT, (4) FEDERAL…

Population Analysis: A Methodology for Understanding Populations in COIN Environments

DTIC Science & Technology

2008-12-01

Psychology is also known for its controversy. Theories such as those of Sigmund Freud and Abraham Maslow remain controversial, and have clouded the...from the Freud’s concept of the id and ego to Marx concept of class struggles ( Freud , 1923) (Marx, 1847). For the purpose of this thesis, we have...8217The bases of social power,’ in D. Cartwright (ed.) Studies in Social Power. Ann Arbor. Freud , S. (1923). The Ego and the Id, Joan Riviere (trans

Dose-Dependent Thresholds of 10-ns Electric Pulse Induced Plasma Membrane Disruption and Cytotoxicity in Multiple Cell Lines

DTIC Science & Technology

2011-01-01

normalized to parallel controls. Flow Cytometry and Confocal Microscopy Upon exposure to 10-ns EP, aliquots of the cellular suspension were added to a tube...Survival data was processed and plotted using GrapherH software (Golden Software, Golden, Colorado). Flow cytometry results were processed in C6 software...Accuri Cytometers, Inc., Ann Arbor, MI) and FCSExpress software (DeNovo Software, Los Angeles, CA). Final analysis and presentation of flow cytometry

Papers Selected for Presentation at the International Symposium on Remote Sensing of Environment (16th) Held at Buenos Aires, Argentina on 2-9 June 1982. Volume 1.

DTIC Science & Technology

1982-06-01

Subsurface Water Parameters Using Radiance Measurements from Space. POOP 022 Limnological Study of the Coastal Lagoon ’Coyuca de Benitez, Guerrero...unlimited. ORGANIZED BY Comisi6n Nacional de Investigaciones Espaciales Buenos Aires, Argentina Environmental Research Institute of Michigan Ann Arbor...Michigan, USA SPONSORED, IN PART, BY ARGENTINA Comando en Jefe de la Fuerza Aerea Argentina Secretarla de Planeamiento de la Presidencia de la Naci6n

IEEE International Symposium on Information Theory (ISIT): Abstracts of Papers, Held in Ann Arbor, Michigan on 6-9 October 1986.

DTIC Science & Technology

1986-10-01

BUZO, and FEDERICO KUHLMANN, Universidad Nacional Autdnoma de Mixico, Facultad de Ingenieria , Divisidn Estudios de Posgrado, P.O. Box 70-256, 04510...unsuccess- ful in this area for a long time. It was felt, e.g., in the voiceband modem industry , that the coding gains achievable by error-correction coding...without bandwidth expansion or data rate reduction, when compared to uncoded modulation. The concept was quickly adopted by industry , and is now becoming

Installation Restoration Program (IRP). Phase 2. Confirmation/Quantification. Stage 1. McEntire Air National Guard Base, Eastover, South Carolina.

DTIC Science & Technology

1986-06-10

Environmental Research Group (ERG) Laboratories of Ann Arbor, Michigan, and a duplicate set sent to OEHL’s laboratory in San Antonio, Texas. The remainder of...sites and well clusters , and the overall sparsity of data points at the base, a water table aquifer potentiometric surface map for the entire base could...L _ MW2-34 The predominant solid wastes disposed at this site were paper and domestic refuse, old wood from demolished structures, general

Self Assembly Nonlinear Optical Effects with Novel Small Metal Cluster Systems

DTIC Science & Technology

2013-07-29

Regents of the University of Michigan 3003 S. State St Ann Arbor, MI 48109 -1274 REPORT DOCUMENTATION PAGE b . ABSTRACT UU c. THIS PAGE UU 2. REPORT TYPE...Journal of Physical Chemistry Letters, (09 2011): 0. doi: 10.1021/jz200738t 10/30/2011 4.00 Travis B . Clark, Meghan E. Orr, Daniel C. Flynn, Theodore...jp2005925 10/30/2011 5.00 Mary Sajini Devadas , Junhyung Kim, Ekkehard Sinn, Dongil Lee, Theodore Goodson, Guda Ramakrishna. Unique Ultrafast Visible

Symposium of Naval Hydrodynamics (14th) held at Ann Arbor, Michigan on August 23-27, 1982,

DTIC Science & Technology

1982-01-01

Chahine -Viscous Effects on the Stability of Cavitating Line Vortices -. 195 Jaakko V. Pylkknen Nuclei and Cavitation 215 Jean -Pierre Le G9fu and Yves...the sectional area of the sheet cavity at • ,this position. . .~ . . * , ILv ’. - ,’ 4 4- ,*-. . ... . 4.4 K"% Nuclei and Cavitation Jean -Pierre Le Goff...the experiments, with analysing .- the results and with running computer programs. Thanks are also due to U H Pinto who developed a substantial part of

The influence of the multi-basic cleavage site of the H5 hemagglutinin on the attenuation, immunogenicity and efficacy of a live attenuated influenza A h5N1 cold-adapted vaccine virus

USDA-ARS?s Scientific Manuscript database

A recombinant live attenuated influenza virus (LAIV) deltaH5N1 vaccine with a modified hemagglutinin (HA) and intact neuraminidase genes from A/Vietnam/1203/04 (H5N1) and the six remaining genome segments from A/Ann Arbor/6/60 (H2N2) cold-adapted (AA ca) virus was attenuated in chickens, mice and fe...

Terahertz Tools Advance Imaging for Security, Industry

NASA Technical Reports Server (NTRS)

2010-01-01

Picometrix, a wholly owned subsidiary of Advanced Photonix Inc. (API), of Ann Arbor, Michigan, invented the world s first commercial terahertz system. The company improved the portability and capabilities of their systems through Small Business Innovation Research (SBIR) agreements with Langley Research Center to provide terahertz imaging capabilities for inspecting the space shuttle external tanks and orbiters. Now API s systems make use of the unique imaging capacity of terahertz radiation on manufacturing floors, for thickness measurements of coatings, pharmaceutical tablet production, and even art conservation.

Environmental Assessment for Tower Construction at the Brandywine Communication Receiver Site, Prince George’s County, Maryland

DTIC Science & Technology

2005-05-01

mobilization . • Place1nent of tower guy wires will be adjusted to avoid construction and disturbance to any wetlands or small tributaries through on...include combustion emissions (VOC, NOx, CO, SO2) and fugitive dust (PM10) from mobile heavy-duty diesel- and gasoline-powered equipment and soil...Pollutant Factors, Mobile Sources (AP 42). 4th Edition, U.S. Environmental Protection Agency, Ann Arbor, Michigan. Total estimated emissions for VOC and

Hemostatic Function of Apheresis Platelets Stored at 4 deg C and 22 deg C

DTIC Science & Technology

2014-05-01

utilized. Thromboxane B2 (TxB2) enzyme immunoassay kits were purchased from Cayman Chemicals (Ann Arbor, MI), and human soluble CD40L (sCD40L) extra...sensitive platinum enzyme linked immunosorbent assay kits were pur chased from eBioscience (Vienna, Austria). CG4+ and CHEM8+ cartridges were purchased from...TruCount tubes (BD Biosciences). Enzyme linked immunosorbent assay Commercially available kits were used to assess sCD40L and TxB2 levels released into

Odor modeling methodology for determining the odor buffer distance for sanitary landfills

DOE Office of Scientific and Technical Information (OSTI.GOV)

Song, Dukman.

1991-01-01

The objective of this study is to create a methodology whereby reductions in off-site odor migrations resulting from operational and design changes in new or expanded sanitary landfills can be evaluated. The Ann Arbor Sanitary Landfill was chosen as a prototype landfill to test a hypothesis for this study. This study is a unique approach to odor prediction at sanitary landfills using surface flux measurements, odor threshold panel measurements, and dispersion modeling. Flux measurements were made at open tipping face, temporary cover, final cover, vents, and composting zones of the Ann Arbor Sanitary Landfill. Surface gas velocities and in-ground concentrationsmore » were determined to allow a quantification of the total and methane gas flow rate. Odor threshold panel measurements were performed to determine the odor intensity in odor units at the corresponding sites. The used the flux and odor panel measurements in the Industrial Source Complex Terrain Model to determine the hourly averaged highest and second highest odor levels at 175 receptors placed at the property boundary and 25 nearby residential locations. Using measured values for velocity, subsurface CH{sub 4} concentration and odor intensity, it was determined that the proposed 1990 operations with a buffer distance of 600 feet provided at least a factor of five protection below 1 o.u. of the odor threshold for all receptors, and dilution protection equal to the historic 1984 operations with a 1,200 feet isolation distance.« less

Perceived difficulty quitting predicts enrollment in a smoking-cessation program for patients with head and neck cancer.

PubMed

Duffy, Sonia A; Scheumann, Angela L; Fowler, Karen E; Darling-Fisher, Cynthia; Terrell, Jeffrey E

2010-05-01

To determine the predictors of participation in a smoking-cessation program among patients with head and neck cancer. This cross-sectional study is a substudy of a larger, randomized trial of patients with head and neck cancer that determined the predictors of smokers' participation in a cessation intervention. Otolaryngology clinics at three Veterans Affairs medical centers (Ann Arbor, MI, Gainesville, FL, and Dallas, TX), and the University of Michigan Hospital in Ann Arbor. 286 patients who had smoked within six months of the screening survey were eligible for a smoking-cessation intervention. Descriptive statistics and bivariate and multivariate logistic regression were used to determine the independent predictors of smokers' participation in an intervention study. Perceived difficulty quitting (as a construct of self-efficacy), health behaviors (i.e., smoking and problem drinking), clinical characteristics (i.e., depression and cancer site and stage), and demographic variables. Forty-eight percent of those eligible participated. High perceived difficulty quitting was the only statistically significant predictor of participation, whereas problem drinking, lower depressive symptoms, and laryngeal cancer site approached significance. Special outreach may be needed to reach patients with head and neck cancer who are overly confident in quitting, problem drinkers, and patients with laryngeal cancer. Oncology nurses are in an opportune position to assess patients' perceived difficulty quitting smoking and motivate them to enroll in cessation programs, ultimately improving quality of life, reducing risk of recurrence, and increasing survival for this population.

Celebrating the International Year of Light in Michigan

NASA Astrophysics Data System (ADS)

Sala, Anca L.; Dreyer, Elizabeth F. C.; Aku-Leh, Cynthia; Jones, Timothy; Nees, John A.; Smith, Arlene

2016-09-01

The 2015 International Year of Light created a wonderful opportunity to bring light and optics events and activities to people of all ages and occupations in Michigan. A large spectrum of events took place; from events held in schools, colleges, and universities targeting various groups of students, to events associated with festivals attended by large crowds. The latter included the Ann Arbor Summer Festival held in June and the Flint Back-to-the-Bricks Festival in August. All events included interactive activities where participants learned hands-on about optics and photonics phenomena and applications. Original demonstrations and kits were developed by the Ann Arbor OSA Local Section and the Optics Society at the University of Michigan, the joint OSA/SPIE student chapter, for use during the events. The activities were funded through the student chapter's SPIE grant for IYL outreach events and corporate sponsorships. Under the name Michigan Light Project, these groups along with local technology enthusiasts and science clubs delivered several events across Michigan. Other events took place throughout the year in Mid-Michigan through the efforts of faculty and students in the Photonics and Laser Technology program at Baker College of Flint. The outreach events targeted students in K-12. Teachers, counselors, and parents also learned about the importance of optics and photonics in society. The activities developed will continue this year and in the future. The paper will provide details on the completed events and activities along with tips for implementing similar activities and outreach partnerships in other areas.

Spatial-Temporal Survey and Occupancy-Abundance Modeling To Predict Bacterial Community Dynamics in the Drinking Water Microbiome

PubMed Central

Pinto, Ameet J.; Schroeder, Joanna; Lunn, Mary; Sloan, William

2014-01-01

ABSTRACT Bacterial communities migrate continuously from the drinking water treatment plant through the drinking water distribution system and into our built environment. Understanding bacterial dynamics in the distribution system is critical to ensuring that safe drinking water is being supplied to customers. We present a 15-month survey of bacterial community dynamics in the drinking water system of Ann Arbor, MI. By sampling the water leaving the treatment plant and at nine points in the distribution system, we show that the bacterial community spatial dynamics of distance decay and dispersivity conform to the layout of the drinking water distribution system. However, the patterns in spatial dynamics were weaker than those for the temporal trends, which exhibited seasonal cycling correlating with temperature and source water use patterns and also demonstrated reproducibility on an annual time scale. The temporal trends were driven by two seasonal bacterial clusters consisting of multiple taxa with different networks of association within the larger drinking water bacterial community. Finally, we show that the Ann Arbor data set robustly conforms to previously described interspecific occupancy abundance models that link the relative abundance of a taxon to the frequency of its detection. Relying on these insights, we propose a predictive framework for microbial management in drinking water systems. Further, we recommend that long-term microbial observatories that collect high-resolution, spatially distributed, multiyear time series of community composition and environmental variables be established to enable the development and testing of the predictive framework. PMID:24865557

Role of Immune Microenvironmental Factors for Improving the IPI-related Risk Stratification of Aggressive B Cell Lymphoma.

PubMed

Gong, Yi; Chen, Rui; Zhang, Xi; Zou, Zhong Min; Chen, Xing Hua

2017-07-01

To investigate the risk stratification of aggressive B cell lymphoma using the immune microenvironment and clinical factors. A total of 127 patients with aggressive B cell lymphoma between 2014 and 2015 were enrolled in this study. CD4, Foxp3, CD8, CD68, CD163, PD-1, and PD-L1 expression levels were evaluated in paraffin-embedded lymphoma tissues to identify their roles in the risk stratification. Eleven factors were identified for further evaluation using analysis of variance, chi-square, and multinomial logistic regression analysis. Significant differences in 11 factors (age, Ann Arbor stage, B symptom, ECOG performance status, infiltrating CD8+ T cells, PD-L1 expression, absolute blood monocyte count, serum lactate dehydrogenase, serum iron, serum albumin, and serum β2-microglobulin) were observed among patient groups stratified by at least two risk stratification methods [International Prognostic Index (IPI), revised IPI, and NCCN-IPI models] (P < 0.05). Concordance rates were high (81.4%-100.0%) when these factors were used for the risk stratification. No difference in the risk stratification results was observed with or without the Ann Arbor stage data. We developed a convenient and inexpensive tool for use in risk stratification of aggressive B cell lymphomas, although further studies on the role of immune microenvironmental factors are needed. Copyright © 2017 The Editorial Board of Biomedical and Environmental Sciences. Published by China CDC. All rights reserved.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Maloney, J.; Kiepper, A.F.; Simonetta, R.J.

The volume examines monitoring and reporting procedures applicable to performance and productivity. The managers of three transit systems of varying sizes discuss how productivity has been increased within their systems. The Pittsburgh discussion reviews a variety of capital projects and management improvement initiatives undertaken by Port Authority Transit, including development of maintenance manuals and an absenteeism reduction project. The Houston discussion describes how the property used a management study to identify needed organizational change and restructuring. The Ann Arbor discussion focuses on a set of service standards and performance indicators adopted to focus the management improvement process.

Direct Detection of Time-Resolved Rabi Oscillationsin a Single Quantum Dot via Resonance Fluorescence

DTIC Science & Technology

2013-03-19

Ware, E. A. Stinaff, D. Gammon, M. F. Doty, A. S . Bracker, D. Gershoni, V. L. Korenev , S . C. Bădescu, Y. Lyanda-Geller, and T. L. Reinecke, Phys. Rev...A SINGLE QUANTUM DOT VIA RESONANCE FLUORESCENCE 5a. CONTRACT NUMBER FA8750-12-2-0333 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR( S ) J...NUMBER CH 7. PERFORMING ORGANIZATION NAME( S ) AND ADDRESS(ES) University of Michigan 450 Church Street Ann Arbor MI 48109-1040 8. PERFORMING

Science and technology review: June 1996

DOE Office of Scientific and Technical Information (OSTI.GOV)

Failor, B.; Stull, S.

1996-06-01

The first feature article is a survey of four research projects showing how theory and modeling efforts by scientist in the Chemistry and Materials Science Directorate at LLNL are advancing the understanding of the property of materials with consideration of underlying structures. The second feature article discusses Livermore and DOE`s Oakland Operations Office teaming up to decontaminate, decommission, and close out--on time and under budget--the Ann Arbor Inertial Confinement Fusion Facility in Michigan. Two research highlights on Mammoth Mountain CO{sub 2} mystery and osteoporosis are also included.

Remote sensing in Michigan for land resource management

NASA Technical Reports Server (NTRS)

Sattinger, I. J.

1972-01-01

This project to demonstrate the application of earth resource survey technology to current problems in Michigan was undertaken jointly by the Environmental Research Institute of Michigan and Michigan State University. Remote sensing techniques were employed to advantage in providing management information for the Pointe Mouillee State Game Area and preparing an impact assessment in advance of the projected construction of the M-14 freeway from Ann Arbor to Plymouth, Michigan. The project also assisted the state government in its current effort to develop and implement a state-wide land management plan.

Hot Carriers in Semiconductors (Proceedings (6th) Held in Scottsdale, Arizona on 23-28 July 1989

DTIC Science & Technology

1989-07-01

Research Red Bank, NJ 07701. We compared with that in bulk material and this is observe a strongly pronounced current controlled (S- observed in our...speaker TuP-1 "Barrier controlled hot carrier cooling in InGaAs/InP quantum wells," U. Cebulla, G. Bacher, A. Forchel, D. Grutzmacher, and W. T. Tsang...10 " Design , fabrication and operation of a hot lectron resonant tunneling transistor," U. K. Reddy, I. Mehdi. R. K. Mains, and G. I. Haddqd Ann Arbor

U.S. Federal research on fisheries and limnology in the Great Lakes through 1964: an annotated bibliography

USGS Publications Warehouse

Hile, Ralph

1966-01-01

The annotated bibliography is preceded by a brief account of the Federal research program in fisheries and limnology in the Great Lakes in 1957-64. The bibliography covers 314 papers by staff members of the Bureau of Commercial Fisheries Biological Laboratory in Ann Arbor, Mich., and 35 by associated scientists with whom the Laboratory had contractual or other cooperative arrangements; included also are patents issued to Laboratory personnel. A roster of Laboratory scientists as of December 31, 1964, is appended.

International Conference on Atomic Physics (12TH) Held at Ann Arbor, Michigan on July 29-August 2, 1990. Abstracts of Contributed Papers.

DTIC Science & Technology

1990-09-26

Bloomfield IV-13 Observation of the 2 S 1/2 - 2D5/ 2 transition in laser cooled trapped Yb + A . S. Bell , H. A . Klein, G. P. Barwood, P. Gill, A . P...peaks is much less than the Doppler limit. a Supported by NSF and ONR 1. W. Bell and A . Bloom. Phys. Rev. Lett. 6. 280 (1961). 2. B. Sheehy et.al...AVAILABILITY OF REPORT 2b. OECLASSIFICATINOWGAIGSHDL Unlimited Distribution A PERFORMING ORGANIZATION REPORT NUMBER(S) S. MONITORING ORGANIZATION

Evaluation of the econo-mist device

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

The Emission Control Technology Division (ECTD) was contacted by the General Services Administration (GSA) concerning a vapor injection device for use with automobile engines. The device is called the Econo-Mist and is a product of the FAP Corporation of Albuquerque, New Mexico. GSA had received information that the Econo-Mist reduced hydrocarbon and carbon monoxide emissions and increased fuel economy. At the request of GSA, ECTD agreed to test the device. A sample of the device was brought to the EPA laboratory in Ann Arbor, Michigan by FAP Corporation personnel on January 13, 1975.

Perceived Difficulty Quitting Predicts Enrollment in a Smoking-Cessation Program for Patients With Head and Neck Cancer

PubMed Central

Duffy, Sonia A.; Scheumann, Angela L.; Fowler, Karen E.; Darling-Fisher, Cynthia; Terrell, Jeffrey E.

2013-01-01

Purpose/Objectives To determine the predictors of participation in a smoking-cessation program among patients with head and neck cancer. Design This cross-sectional study is a substudy of a larger, randomized trial of patients with head and neck cancer that determined the predictors of smokers’ participation in a cessation intervention. Setting Otolaryngology clinics at three Veterans Affairs medical centers (Ann Arbor, MI, Gainesville, FL, and Dallas, TX), and the University of Michigan Hospital in Ann Arbor. Sample 286 patients who had smoked within six months of the screening survey were eligible for a smoking-cessation intervention. Methods Descriptive statistics and bivariate and multivariate logistic regression were used to determine the independent predictors of smokers’ participation in an intervention study. Main Research Variables Perceived difficulty quitting (as a construct of self-efficacy), health behaviors (i.e., smoking and problem drinking), clinical characteristics (i.e., depression and cancer site and stage), and demographic variables. Findings Forty-eight percent of those eligible participated. High perceived difficulty quitting was the only statistically significant predictor of participation, whereas problem drinking, lower depressive symptoms, and laryngeal cancer site approached significance. Conclusions Special outreach may be needed to reach patients with head and neck cancer who are overly confident in quitting, problem drinkers, and patients with laryngeal cancer. Implications for Nursing Oncology nurses are in an opportune position to assess patients’ perceived difficulty quitting smoking and motivate them to enroll in cessation programs, ultimately improving quality of life, reducing risk of recurrence, and increasing survival for this population. PMID:20439219

A cancer center's approach to engaging African American men about cancer: the men's fellowship breakfast, Southeastern Michigan, 2008-2014.

PubMed

Langford, Aisha T; Griffith, Derek M; Beasley, Derrick D; Braxton, Effat Id-Deen

2014-09-25

Despite disproportionate rates of cancer morbidity and mortality among African American men, few community-based efforts have been developed and sustained to educate African American men about cancer. The University of Michigan Comprehensive Cancer Center implemented a series of breakfasts to improve cancer awareness, screening, and education among African American men. This article describes the rationale for and history of the community intervention. The 21 breakfasts were held from 2008 through mid-2014 in Ypsilanti and Ann Arbor, Michigan. Ypsilanti ranks below Michigan and the nation on most socioeconomic indicators, although most residents are high school graduates (88% in Ypsilanti and 96.5% in Ann Arbor). African American men in Ypsilanti have higher death rates for diseases associated with poor diet and inadequate physical activity compared with Ypsilanti whites and general populations in Michigan and the nation. We conducted a multicomponent qualitative process evaluation including staff meetings, conversations with participants, and focus groups. We collected 425 post-event surveys to evaluate the breakfasts quantitatively. Participants were African American (85%), were aged 51 to 70 years (54%), had health insurance (89%), and had some college education (38%). Fifty-three percent of participants reported interest in the breakfast topics including nutrition; 46%, prostate cancer; 34%, colorectal cancer, and 32%, pain management; 62% reported willingness to participate in a clinical trial. African American men are interested in learning about health and are willing to attend a health-focused breakfast series. The Men's Fellowship Breakfast is a promising strategy for bringing men together to discuss cancer screening and risk reduction.

AFOSR/ONR (Air Force Office of Scientific Research/Office of Naval Research) Contractors’ Meeting - Combustion Rocket Propulsion Diagnostics of Reacting Flow Held in Ann Arbor, Michigan on June 19-23, 1989

DTIC Science & Technology

1989-06-19

ORGANIZATION NAME(S) AND AOORESS(ES) L PERJORMING ORGANIZATION S Air Force Office of Scientific Research REPORT NUMBER Building 410 AF06 IR 1 7 1 j Bolling...AFB DC 20332-6448 Office of Naval Research , Arlington VA 22217-5000 9. SFONSOtrU/MONITOPING AGENCY NAME(S) AND ADORESS(ES) 10. SPONSORINGIMONITORING...CODE Approved for public release; distribution is unlimited 13. ABSTRACT (Muxmmum 200 words*) Abstracts are given for research on airbreathing

Bright Two-Photon Emission and Ultra-Fast Relaxation Dynamics in a DNA-Templated Nanocluster Investigated by Ultra-Fast Spectroscopy

DTIC Science & Technology

2012-01-01

Michigan 3003 S. State St Ann Arbor, MI 48109 -1274 REPORT DOCUMENTATION PAGE b . ABSTRACT UU c. THIS PAGE UU 2. REPORT TYPE New Reprint 17. LIMITATION OF...Figure 1: Steady state absorption for Au25 Au55, Au140, Au2406 and Mie theory calculation using parameter similar to Au25.7 B . Emission Mechanism of...short-lived (hundreds of fs), and it is most likely to be associated with the metal core (State B ).7,17 The near-infrared emission is related to the

Status, Emerging Ideas and Future Directions of Turbulence Modeling Research in Aeronautics

NASA Technical Reports Server (NTRS)

Duraisamy, Karthik; Spalart, Philippe R.; Rumsey, Christopher L.

2017-01-01

In July 2017, a three-day Turbulence Modeling Symposium sponsored by the University of Michigan and NASA was held in Ann Arbor, Michigan. This meeting brought together nearly 90 experts from academia, government and industry, with good international participation, to discuss the state of the art in turbulence modeling, emerging ideas, and to wrestle with questions surrounding its future. Emphasis was placed on turbulence modeling in a predictive context in complex problems, rather than on turbulence theory or descriptive modeling. This report summarizes many of the questions, discussions, and conclusions from the symposium, and suggests immediate next steps.

[The old man and the I sea U : Essay on faith, fate and evidence - after the manner of Hemingway].

PubMed

Lewandowski, K; Bartlett, R H

2017-01-01

Robert Bartlett, emeritus Professor of surgery at the University of Michigan in Ann Arbor, USA, transformed classical works of world literature (Charles Dickens: A Christmas Carol, Lewis Carroll: Alice in Wonderland) into teaching aids for advanced training in intensive care medicine. He recently turned his hand to the well-known work of Ernest Hemingway: the Nobel Prize winning novel The Old Man and the Sea. Subsequent to Robert Bartlett's essay this article provides background information and comments on the current problems in modern intensive care medicine addressed in his essay.

Modulation Transfer Function Analysis of Kelvin Wakes and Ambient Wave Images

DTIC Science & Technology

1991-09-01

O n s ŕ-6 1 ed.’.. to a vvff" I Pý,o r . ~, -t~m t . t," for ,. ,eo~rV (I0, msv os . fl *vo ~tn flq **ta ~ cfK tat W~.4 ~ n ?’Ct of -t¶~ .19 t.#aw...TYPE AND DATES COVERED September 1991 Technical 10/J/90 - 9/30/91 4. TITLE AND SUBTITLE S. FUNDIN i NUMBERS Modulation Transfer Function Analysis of ...ADDRESSUES) 1. PERFORMING ORGANIZATION REPORT NUMBER Environmental Research Institute of Michigan (ERIM) P.O. Box 134001 207500-7-T Ann Arbor, MI 48113-4001 9

Implications of infiltrating immune cells within bone marrow of patients with diffuse large B-cell lymphoma.

PubMed

Jeong, Juhyeon; Oh, Eun Ji; Yang, Woo Ick; Kim, Soo Jeong; Yoon, Sun Och

2017-06-01

The implications of infiltrating immune cells, especially T cells and macrophages, in the bone marrow (BM) microenvironment of patients with diffuse large B-cell lymphoma (DLBCL) have rarely been studied. We aimed to investigate the significance of infiltrating immune cells in the BM microenvironment as a prognostic factor for DLBCL patients. Using the initial pretreatment BM biopsy obtained from 198 DLBCL patients, we semiquantitatively evaluated CD3+ T cells, CD8+ T cells, and CD163+ macrophages that infiltrate into the paratrabecular and interstitial areas of BM by immunohistochemistry and analyzed their clinicopathological and prognostic implications. Levels of infiltrating CD3+ T cells, CD8+ T cells, and CD163+ macrophages were significantly higher in BM with DLBCL involvement (BMI-positive group) than in that without DLBCL involvement (BMI-negative group). Infiltration of CD8+ T cells significantly increased in cases with advanced Ann Arbor stage, elevated lactate dehydrogenase level, extranodal site involvement ≥2 sites, higher Eastern Cooperative Oncology Group performance status, and higher International Prognostic Index (IPI) risk. High levels of CD3+ T cells were significantly associated with age ≤60, and high levels of CD163+ macrophages were associated with advanced Ann Arbor stage and higher IPI risk. High infiltration of CD8+ T cells was significantly related to inferior overall and recurrence-free survival rate, even in the BMI-negative group. High infiltration of CD8+ T cells within the pretreatment BM was related to poor prognosis, and might be a useful prognostic factor of DLBCL patients. Therefore, evaluation of CD8+ T cells is helpful for predicting prognosis in initial pretreatment BM biopsy of DLBCL patients. Copyright © 2017 Elsevier Inc. All rights reserved.

A Cancer Center’s Approach to Engaging African American Men About Cancer: The Men’s Fellowship Breakfast, Southeastern Michigan, 2008–2014

PubMed Central

Griffith, Derek M.; Beasley, Derrick D.; Braxton, Effat Id-Deen

2014-01-01

Background Despite disproportionate rates of cancer morbidity and mortality among African American men, few community-based efforts have been developed and sustained to educate African American men about cancer. The University of Michigan Comprehensive Cancer Center implemented a series of breakfasts to improve cancer awareness, screening, and education among African American men. This article describes the rationale for and history of the community intervention. Community Context The 21 breakfasts were held from 2008 through mid-2014 in Ypsilanti and Ann Arbor, Michigan. Ypsilanti ranks below Michigan and the nation on most socioeconomic indicators, although most residents are high school graduates (88% in Ypsilanti and 96.5% in Ann Arbor). African American men in Ypsilanti have higher death rates for diseases associated with poor diet and inadequate physical activity compared with Ypsilanti whites and general populations in Michigan and the nation. Methods We conducted a multicomponent qualitative process evaluation including staff meetings, conversations with participants, and focus groups. We collected 425 post-event surveys to evaluate the breakfasts quantitatively. Outcomes Participants were African American (85%), were aged 51 to 70 years (54%), had health insurance (89%), and had some college education (38%). Fifty-three percent of participants reported interest in the breakfast topics including nutrition; 46%, prostate cancer; 34%, colorectal cancer, and 32%, pain management; 62% reported willingness to participate in a clinical trial. Interpretation African American men are interested in learning about health and are willing to attend a health-focused breakfast series. The Men’s Fellowship Breakfast is a promising strategy for bringing men together to discuss cancer screening and risk reduction. PMID:25254982

Characterization of anti-erythrocyte autoantibodies in non-Hodgkin's lymphoma patients in Brazil.

PubMed

Barjas de Castro, M L; Locatelli, M F; de Castilho, L M; de Souza, C A

1998-01-01

The existence of an association between autoimmune phenomena and lymphoproliferative neoplasms is well known. In Campinas at the University Hospital, seventy-seven adult patients with non-Hodgkin's lymphoma (NHL) were studied at diagnosis. The histological subgroup of NHL was performed using Kiel criteria and all patients were characterized by clinical and laboratory examinations according to the Ann Arbor staging. The results of the immunohaematological evaluation of our patients with NHL showed that: 28% presented erythrocyte autoantibodies (auto anti-I or auto-IgG without specificity) but only one developed haemolytic anaemia. There was a weak correlation between low-grade lymphoma and erythrocyte autoantibodies.

A novel application of the MIRC repository in medical education.

PubMed

Roth, Christopher J; Weadock, William J; Dipietro, Michael A

2005-06-01

Medical students on the radiology elective in our institution create electronic presentations to present to each other as part of the requirements for the rotation. Access was given to previous students' presentations via the web-based system, Medical Imaging Resource Center (MIRC) project, created and supported by the Radiological Society of North America (RSNA). RadPix Power 2 MIRC (Weadock Software, LLC, Ann Arbor, MI) software converted the Microsoft PowerPoint (Redmond, WA) presentations to a MIRC-compatible format. The textual information on each slide is searchable across the entire MIRC database. Future students will be able to benefit from the work of their predecessors.

Combined Hydrophobic/Oleophobic Membrane Separation and Extraction for Fuel Treatment (Briefing Charts)

DTIC Science & Technology

2015-03-24

Institute, Dayton, OH 45469 4 California State University, Long Beach, CA 90840 5 University of Michigan, Ann Arbor, MI 48109 2 Acknowledgements Ms. Yvonne...ppm) C2 Thiophenes 0.3 C3-C4 Thiophenes 1.4 C5 Thiophenes 3.7 C6 Thiophenes 3.5 C7 Thiophenes 4.1 C8 -C9 Thiophenes 2.9 C10 Thiophenes 0.6 C11...Thiophenes 6.3 C6 Thiophenes 6.1 C7 Thiophenes 5.8 C8 -C9 Thiophenes 4.9 C10 Thiophenes 1.3 C11 Thiophenes 0.9 C12+ Thiophenes 2.0 Standard Grade RP-1 (Errors

Two nominated for Science Board

NASA Astrophysics Data System (ADS)

President Ronald Reagan plans to nominate Thomas B. Day and James J. Duderstadt to the National Science Board (NSB), according to an announcement by the National Science Foundation (NSF). Day, a theoretical and experimental high-energy physicist, is president of San Diego State University, San Diego, Calif. Duderstadt is a nuclear engineer and is dean of the College of Engineering of the University of Michigan, Ann Arbor.The 24-member NSB is the policy-making body of the NSF. Current members of the NSB include Peter T. Flawn, a geologist who is president of the University of Texas at Austin, and William A. Nierenberg, director of the Scripps Institution of Oceanography at the University of California, San Diego.

AFOSR/ONR Contractors’ Meeting on Combustion Rocket Propulsion Diagnostics of Reacting Flow Held in Ann Arbor, Michigan on 19-23 June 1989

DTIC Science & Technology

1989-06-19

Applications of Laser-Induced Exciplex Fluorescence to Fuel Spray and Single Droplet Vaporization, L A Melton...m 0000 a a Tm ol m 0 0 4. LL o WWN 64 0 4N N WXX 0 :1N 2 0 0ŕ r. ’ 4 00 4-4 -00 co "i 0 W o> ca 0. 0 C3 (V CLw 1 o ) W4 V)V tn A Ao6 - I 0014CJ...particular turbulent str zture OL , the outer edge (air side) of the shear layer can be followed from frame to frame. The 10 us time separation between frames

Overview of the International Symposium on Eurasian Ruffe (Gymnocephalus cernuus) Biology, Impacts, and Control

USGS Publications Warehouse

Gunderson, Jeffrey L.; Klepinger, Michael R.; Bronte, Charles R.; Marsden, J. Ellen

1998-01-01

The International Symposium on the Biology and Management of Ruffe was organized to address the potential threat ruffe pose to North American fisheries. Scientists in diverse disciplines from Eurasia and North America were brought together in an attempt to examine all aspects of the North American invasion of ruffe, and to highlight the effects of similar introductions in Europe and Asia. The symposium, sponsored by the Minnesota and Michigan Sea Grant College programs, featured 48 oral and poster presentations and was held in Ann Arbor, Michigan, during 21-23 March 1997. Papers from the symposium are published in a special section of this issue of the Journal of Great Lakes Research.

Radiation-induced chondrosarcoma of the maxilla 7-year after combined chemoradiation for tonsillar lymphoma.

PubMed

Mohammadianpanah, M; Gramizadeh, B; Omidvari, Sh; Mosalaei, A

2004-01-01

Radiation-induced sarcoma is a rare complication of radiation therapy. We report a case of radiation-induced chondrosarcoma of the maxilla. An 80-year-old Persian woman developed radiation-induced chondrosarcoma of the left maxilla 7 years after combined chemotherapy and external beam radiation therapy for the Ann Arbor stage IE malignant lymphoma of the right tonsil. She underwent suboptimal tumour resection and died due to extensive locoregional disease 8 months later. An English language literature search of Medline using the terms chondrosarcoma, radiation-induced sarcoma and maxilla revealed only one earlier reported case. We describe the clinical and pathological features of this case and review the literature on radiation-induced sarcomas.

International Conference on Atomic Physics: Abstracts of Contributed Papers (12th) Held in Ann Arbor, Michigan on 29 July-3 August 1990

DTIC Science & Technology

1990-09-26

A . Bloomfield IV-13 Obsenation of the 2SI12 - 2D51 2 transition in laser cooled trapped Y7) 4 A . S. Bell , H. A . Klein, G. P...discussed. I. H A Klein, A S Bell , G P Barwood and P Gill, Appl. Phys B 0, 13 (1990). 2. H A Klein, A S Bell , 0 P Barwood, P Gill and W R C Rowley...H.C.W. IV-6; X-1 Abu-Jafar, M. VII-41 Belkacem, A . VIII-23 Abutaleb, M. VIII-19 Bell , A.S. IV-13 Adachi, H. IX-1 Bengtsson, J. VIII-12 Aggarwal,

Molecular Characterization of CTR-type Copper Transporters in an Oceanic Diatom, Thalassiosira oceanica 1005

NASA Astrophysics Data System (ADS)

Kong, L.; Price, N. M.

2016-02-01

Copper is an essential micronutrient for phytoplankton growth because of its role as a redox cofactor in electron transfer proteins in photosynthesis and respiration, and a potentially limiting resource in parts of the open sea. Thalassiosira oceanica 1005 can grow at inorganic copper concentrations varying from 10 fmol/L to 10 nmol/L by regulating copper uptake across plasma membrane. Four putative CTR-type copper transporter genes (ToCTR1, ToCTR2, ToCTR3.1 and ToCTR3.2) were identified by BLASTP search against the T. oceanica genome. Predicted gene models were revised by assembled mRNA sequencing transcripts and updated gene models contained all conserved features of characterized CTR-type copper transporters. ToCTR3.1 and ToCTR3.2 may arise from one another by gene duplication as they shared a sequence similarity of 97.6% with a peptide insertion of 5 amino acids at N-terminus of ToCTR3.1. The expression of ToCTR1, ToCTR2 and ToCTR3.1/3.2 was upregulated in low copper concentrations, but only ToCTR3.1/3.2 showed a significant increase (2.5 fold) in copper-starved cells. Both ToCTR3.1 and ToCTR3.2 restored growth of a yeast double mutant, Saccharomyces cerevisiae ctr1Δctr3Δ, in copper deficient medium. GFP-fused ToCTR expression showed that some ToCTR3.1 localized to the plasma membrane but a large portion was retained in the endoplasmic reticulum. Inefficient targeting of ToCTR3.1 to the yeast outer membrane may explain poorer growth compared to the Saccharomyces native ScCTR1 transformant. Thus, diatom CTR genes encoding CTR-type copper transporters show high-affinity copper uptake and their regulation may enable diatoms to survive in ocean environments containing a wide range of copper concentrations.

Evidence that one is more likely to see the aurora near Moscow than near Ann Arbor

NASA Astrophysics Data System (ADS)

Liemohn, Michael; Immel, Thomas; Katus, Roxanne

We present a superposed epoch analysis of solar wind drivers and geomagnetic index responses during magnetic storms, categorized as a function of universal time (UT) of the storm peak, to investigate the dependency of storm intensity on UT. Storms with Dst minimum less than - 100 nT were identified in the 1970 - 2012 era (totaling 310 events), covering four solar cycles. The storms were classified into 6 groups based on the UT of the minimum Dst (36 to 82 events per bin), then each grouping was superposed on a timeline that aligns the time of the minimum Dst. Fifteen different quantities were considered, seven solar wind parameters and eight activity indices derived from ground-based magnetometers. Statistical analyses of the superposed means against each other (between the different UT groupings) were conducted to determine the mathematical significance of similarities and differences in the time series plots. It was found that most of the solar wind parameters have essentially no significant difference between the UT groupings, as expected. The exception is solar wind velocity, which appears to be bifurcated into two levels with three of the UT groupings systematically faster than the other three (although, interestingly, not three consecutive UT bins). The geomagnetic activity indices, however, all show statistically significant differences with UT during the main phase and/or early recovery phase. Specifically, the 16, 20, and 00 UT groupings are stronger storms than those in the other UT bins. That is, storms are stronger when the Asian sector is on the nightside (American sector on the dayside) during the main phase. An inference from these findings, therefore, is that one is more likely to see the aurora near Moscow in Russia than near Ann Arbor, Michigan in the United States, even though these two cities have very similar magnetic latitudes (52 degrees).

LeCTR1, a Tomato CTR1-Like Gene, Demonstrates Ethylene Signaling Ability in Arabidopsis and Novel Expression Patterns in Tomato1

PubMed Central

Leclercq, Julie; Adams-Phillips, Lori C.; Zegzouti, Hicham; Jones, Brian; Latché, Alain; Giovannoni, James J.; Pech, Jean-Claude; Bouzayen, Mondher

2002-01-01

LeCTR1 was initially isolated by both differential display reverse transcriptase-polymerase chain reaction screening for tomato (Lycopersicon esculentum) fruit ethylene-inducible genes and through homology with the Arabidopsis CTR1 cDNA. LeCTR1 shares strong nucleotide sequence homology with Arabidopsis CTR1, a gene acting downstream of the ethylene receptor and showing similarity to the Raf family of serine/threonine protein kinases. The length of the LeCTR1 transcribed region from ATG to stop codon (12,000 bp) is more than twice that of Arabidopsis CTR1 (4,700 bp). Structural analysis reveals perfect conservation of both the number and position of introns and exons in LeCTR1 and Arabidopsis CTR1. The introns in LeCTR1 are much longer, however. To address whether this structural conservation is indicative of functional conservation of the corresponding proteins, we expressed LeCTR1 in the Arabidopsis ctr1-1 (constitutive triple response 1) mutant under the direction of the 35S promoter. Our data clearly show that ectopic expression of LeCTR1 in the Arabidopsis ctr1-1 mutant can restore normal ethylene signaling. The recovery of normal ethylene sensitivity upon heterologous expression of LeCTR1 was also confirmed by restored glucose sensitivity absent in the Arabidopsis ctr1-1 mutant. Expression studies confirm ethylene responsiveness of LeCTR1 in various tissues, including ripening fruit, and may suggest the evolution of alternate regulatory mechanisms in tomato versus Arabidopsis. PMID:12427980

Gene duplication and neo-functionalization in the evolutionary and functional divergence of the metazoan copper transporters Ctr1 and Ctr2.

PubMed

Logeman, Brandon L; Wood, L Kent; Lee, Jaekwon; Thiele, Dennis J

2017-07-07

Copper is an essential element for proper organismal development and is involved in a range of processes, including oxidative phosphorylation, neuropeptide biogenesis, and connective tissue maturation. The copper transporter (Ctr) family of integral membrane proteins is ubiquitously found in eukaryotes and mediates the high-affinity transport of Cu + across both the plasma membrane and endomembranes. Although mammalian Ctr1 functions as a Cu + transporter for Cu acquisition and is essential for embryonic development, a homologous protein, Ctr2, has been proposed to function as a low-affinity Cu transporter, a lysosomal Cu exporter, or a regulator of Ctr1 activity, but its functional and evolutionary relationship to Ctr1 is unclear. Here we report a biochemical, genetic, and phylogenetic comparison of metazoan Ctr1 and Ctr2, suggesting that Ctr2 arose over 550 million years ago as a result of a gene duplication event followed by loss of Cu + transport activity. Using a random mutagenesis and growth selection approach, we identified amino acid substitutions in human and mouse Ctr2 proteins that support copper-dependent growth in yeast and enhance copper accumulation in Ctr1 -/- mouse embryonic fibroblasts. These mutations revert Ctr2 to a more ancestral Ctr1-like state while maintaining endogenous functions, such as stimulating Ctr1 cleavage. We suggest key structural aspects of metazoan Ctr1 and Ctr2 that discriminate between their biological roles, providing mechanistic insights into the evolutionary, biochemical, and functional relationships between these two related proteins. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

Seven Enigmas: The Universe Within and Without

NASA Astrophysics Data System (ADS)

Cogswell, J.

2013-04-01

Seven Enigmas was a multi-media collaborative performance work celebrating the capacity of the human spirit to imaginatively explore the universe outside and within. It was co-created by dancer and choreographer Peter Sparling and the author, a visual artist. Inspired by the spatial movement and gestural power of seven dance miniatures choreographed by Sparling, I mapped out a multimedia sculptural installation for our stage production: objects and surfaces in motion with human bodies, along with film and video projections. The piece was realized through the joint efforts of a team of artists, scientists, dancers, and musicians, and performed at the Power Center for the Performing Arts in Ann Arbor in July and again in September 1997 by the Peter Sparling Dance Company.

The society of thoracic surgeons.

PubMed

Alley, R D

1976-11-01

The opportunity to discuss evolving socioeconomic issues affecting the medical profession as a whole was rejected in favor of personalizing The Society of Thoracic Surgeons for a membership that has grown to almost 1,500 persons. Drawing on historical insights gained through participation in the origin, growth, and development of The Society, I take readers behind the scenes and introduce to them the many people and vast resources that serve our membership. Particular attention is focused on the services of The Society's headquarters office, which is housed and managed by Smith, Bucklin and Associates, Inc, of Chicago, a multiple-association management firm that serves 60 national organizations; on our prestigious publisher, Little, Brown and Company of Boston; and on its link with our Editorial Office in Ann Arbor.

Willie Hobbs Moore (1934-1994): The First Female African American Physicist

NASA Astrophysics Data System (ADS)

Mickens, Ronald

2011-03-01

We discuss the life and career of Willie Hobbs Moore, the first African American woman to receive a doctorate degree in physics. This achievement occurred in June 1972 at the University of Michigan, Ann Arbor, MI. Her dissertation, directed by the renowned spectroscopist Samuel Krimm, was on the subject of ``A Vibrational Analysis of Secondary Chlorides," and focused on a theoretical analysis of the secondary chlorides for polyvinal-chlorine polymers. From 1972--1977, she, Krimm, and collaborators published more than thirty papers on this and related research issues. In addition to an overview of her family background, her careers as a research physicist and scientist working in various industrial laboratories, we discuss the obstacles and successes she encountered at various stages of her life.

Effect of Thermal Conductivity on the Knudsen Layer at Ablative Surfaces

DTIC Science & Technology

2007-03-23

Bagnoud Building, 1320 Beam Ave., Ann Arbor, MI 48109 J.-L. Cambier Air Force Research Laboratory, 10 East Saturn Blvd...x zyx x b V V VVuV V n VV Vf π β π r (2) 5 ( ) ( ) ⎪⎭ ⎪ ⎬ ⎫ ⎪⎩ ⎪ ⎨ ⎧ ⎥ ⎥ ⎦ ⎤ ⎢ ⎢ ⎣ ⎡ ⋅⎟ ⎟ ⎠ ⎞ ⎜ ⎜ ⎝ ⎛ − ++− ⋅ − ⋅ ⋅ −⋅⋅= T...dx d V VVuV V uVVVVfnVf zyxxTMu ln2 5)(1)()( 2 1 222 1 1 1 ν rr , (3) [ ] ⎟⎟ ⎟ ⎠ ⎞ ⎜⎜ ⎜ ⎝ ⎛ ++− −⋅⎟⎟ ⎠ ⎞ ⎜⎜ ⎝ ⎛

Expression profiles of a MhCTR1 gene in relation to banana fruit ripening.

PubMed

Hu, Huei-Lin; Do, Yi-Yin; Huang, Pung-Ling

2012-07-01

The banana (Musa spp.) is a typical climacteric fruit of high economic importance. The development of bananas from maturing to ripening is characterized by increased ethylene production accompanied by a respiration burst. To elucidate the signal transduction pathway involved in the ethylene regulation of banana ripening, a gene homologous to Arabidopsis CTR1 (constitutive triple response 1) was isolated from Musa spp. (Hsien Jin Chiao, AAA group) and designated as MhCTR1. MhCTR1 spans 11.5 kilobases and consists of 15 exons and 14 introns with consensus GT-AG nucleotides situated at their boundaries. MhCTR1 encodes a polypeptide of 805 amino acid residues with a calculated molecular weight of 88.6 kDa. The deduced amino acid sequence of MhCTR1 demonstrates 55%, 56% and 55% homology to AtCTR1, RhCTR1, and LeCTR1, respectively. MhCTR1 is expressed mostly in the mature green pulp and root organs. During fruit development MhCTR1 expression increases just before ethylene production rises. Moreover, MhCTR1 expression was detected mainly in the pulps at ripening stage 3, and correlated with the onset of peel yellowing, while MhCTR1 was constitutively expressed in the peels. MhCTR1 expression could be induced by ethylene treatment (0.01 μL L(-1)), and MhCTR1 expression decreased in both peel and pulp 24 h after treatment. Overall, changes observed in MhCTR1 expression in the pulp closely related to the regulation of the banana ripening process. Copyright © 2012. Published by Elsevier Masson SAS.

Cloning and characterisation of two CTR1-like genes in Cucurbita pepo: regulation of their expression during male and female flower development.

PubMed

Manzano, Susana; Martínez, Cecilia; Gómez, Pedro; Garrido, Dolores; Jamilena, Manuel

2010-12-01

Ethylene is an essential regulator of flower development in Cucurbita pepo, controlling the sexual expression, and the differentiation and maturation of floral organs. To study the action mechanism of ethylene during the male and female flower development, we have identified two CTR1 homologues from C. pepo, CpCTR1 and CpCTR2, and analysed their expressions during female and male flower development and in response to external treatments with ethylene. CpCTR1 and CpCTR2 share a high homology with plant CTR1-like kinases, but differ from other related kinases such as the Arabidopsis EDR1 and the tomato LeCTR2. The C-terminal ends of both CpCTR1 and CpCTR2 have all the conserved motifs of Ser/Thr kinase domains, including the ATP-binding signature and the protein kinase active site consensus sequence, which suggests that CpCTR1 and CpCTR2 could have the same function as CTR1 in ethylene signalling. The transcripts of both genes were detected in different organs of the plant, including roots, leaves and shoots, but were mostly accumulated in mature flowers. During the development of male and female flowers, CpCTR1 and CpCTR2 expressions were concomitant with ethylene production, which indicates that both genes could be upregulated by ethylene, at least in flowers. Moreover, external treatments with ethylene, although did not alter the expression of these two genes in seedlings and leaves, were able to upregulate their expression in flowers. In the earlier stages of flower development, when ethylene production is very low, the expression of CpCTR1 and CpCTR2 is higher in male floral organs, which agrees with the role of these genes as negative regulators of ethylene signalling, and explain the lower ethylene sensitivity of male flowers in comparison with female flowers. The function of the upregulation of these two genes in later stages of female flower development, when the production of ethylene is also increased, is discussed.

Rice CONSTITUTIVE TRIPLE-RESPONSE2 is involved in the ethylene-receptor signalling and regulation of various aspects of rice growth and development

PubMed Central

Wen, Chi-Kuang

2013-01-01

In Arabidopsis, the ethylene-receptor signal output occurs at the endoplasmic reticulum and is mediated by the Raf-like protein CONSTITUTIVE TRIPLE RESPONSE1 (CTR1) but is prevented by overexpression of the CTR1 N terminus. A phylogenic analysis suggested that rice OsCTR2 is closely related to CTR1, and ectopic expression of CTR1p:OsCTR2 complemented Arabidopsis ctr1-1. Arabidopsis ethylene receptors ETHYLENE RESPONSE1 and ETHYLENE RESPONSE SENSOR1 physically interacted with OsCTR2 on yeast two-hybrid assay, and green fluorescence protein-tagged OsCTR2 was localized at the endoplasmic reticulum. The osctr2 loss-of-function mutation and expression of the 35S:OsCTR2 1–513 transgene that encodes the OsCTR2 N terminus (residues 1–513) revealed several and many aspects, respectively, of ethylene-induced growth alteration in rice. Because the osctr2 allele did not produce all aspects of ethylene-induced growth alteration, the ethylene-receptor signal output might be mediated in part by OsCTR2 and by other components in rice. Yield-related agronomic traits, including flowering time and effective tiller number, were altered in osctr2 and 35S:OsCTR2 1–513 transgenic lines. Applying prolonged ethylene treatment to evaluate ethylene effects on rice without compromising rice growth is technically challenging. Our understanding of roles of ethylene in various aspects of growth and development in japonica rice varieties could be advanced with the use of the osctr2 and 35S:OsCTR2 1–513 transgenic lines. PMID:24006427

Advanced Physics Labs and Undergraduate Research: Helping Them Work Together

NASA Astrophysics Data System (ADS)

Peterson, Richard W.

2009-10-01

The 2009 Advanced Lab Topical Conference in Ann Arbor affirmed the importance of advanced labs that teach crucial skills and methodologies by carefully conducting a time-honored experiment. Others however argued that such a constrained experiment can play a complementary role to more open-ended, project experiences. A genuine ``experiment'' where neither student or faculty member is exactly sure of the best approach or anticipated result can often trigger real excitement, creativity, and career direction for students while reinforcing the advanced lab and undergraduate research interface. Several examples are cited in areas of AMO physics, optics, fluids, and acoustics. Colleges and universities that have dual-degree engineering, engineering physics, or applied physics programs may especially profit from interdisciplinary projects that utilize optical, electromagnetic, and acoustical measurements in conjunction with computational physics and simulation.

PERMEABILITY OF BACTERIAL SPORES II.

PubMed Central

Gerhardt, Philipp; Black, S. H.

1961-01-01

Gerhardt, Philipp (University of Michigan, Ann Arbor) and S. H. Black. Permeability of bacterial spores. II. Molecular variables affecting solute permeation. J. Bacteriol. 82:750–760. 1961.—More than 100 compounds were tested for their uptake by dormant spores of a bacillus. The extent of penetration was found to be dependent on at least three molecular properties: (i) The dissociation of electrolytes usually resulted in high or low uptake predictable from their charge. (ii) Lipid insolubility restricted permeation of small molecules. (iii) The molecular weight of unsubstituted glycol and sugar polymers exponentially limited penetration to eventual exclusion at mol wt above 160,000. The results were plotted as a generalized curve, calculations from which permitted an interpretation that the effective spore surface contains pores varying in diameter from 10 to 200 A. PMID:13897940

Remote sensing in Michigan for land resource management: Highway impact assessment

NASA Technical Reports Server (NTRS)

1972-01-01

An existing section of M-14 freeway constructed in 1964 and a potential extension from Ann Arbor to Plymouth, Michigan provided an opportunity for investigating the potential uses of remote sensing techniques in providing projective information needed for assessing the impact of highway construction. Remote sensing data included multispectral scanner imagery and aerial photography. Only minor effects on vegetation, soils, and land use were found to have occurred in the existing corridor. Adverse changes expected to take place in the corridor proposed for extension of the freeway can be minimized by proper design of drainage ditches and attention to good construction practices. Remote sensing can be used to collect and present many types of data useful for highway impact assessment on land use, vegetation categories and species, soil properties and hydrologic characteristics.

[Hodgkin's disease with esophageal involvement].

PubMed

Njeh, M; Yengui, N; Tahri, N; Kchaou, M; Sellami, A; Jlidi, R; Krichen, M S

2000-10-01

Esophageal involvement in Hodgkin's disease, commonly known as a belated localization of the advanced forms, has been seldom reported (3 to 5% in post-mortem series and 0.7% in clinical series). We report the case of a 61-year-old man who had an esophagus localization revealing Hodgkin's disease stage IV EBb of Ann Arbor classification. The originality of this case was represented by: the revelation mode of the esophageal involvement such as dysphagia and upper gastrointestinal bleeding; the localization at the distal third of the esophagus with contiguous involvement of the gastric fundus; the absence of mediastinal nodes showing the primitive character of the esophageal injury. This observation incites us to consider Hodgkin's disease in the list of differential diagnoses of tumoral dysphagia, even if there was no ganglionic and/or visceral localization of the disease.

Theme section for 36th International Symposium for Remote Sensing of the Environment in Berlin

NASA Astrophysics Data System (ADS)

Trinder, John; Waske, Björn

2016-09-01

The International Symposium for Remote Sensing of the Environment (ISRSE) is the longest series of international conferences held on the topic of Remote Sensing, commencing in Ann Arbor, Michigan USA in 1962. While the name of the conference has changed over the years, it is regularly held approximately every 2 years and continues to be one of the leading international conferences on remote sensing. The latest of these conferences, the 36th ISRSE, was held in Berlin, Germany from 11 to 15 May 2015. All complete papers from the conference are available in the ISPRS International Archives of the Photogrammetry, Remote Sensing and Spatial Information Sciences at http://www.int-arch-photogramm-remote-sens-spatial-inf-sci.net/XL-7-W3/index.html.

GLOBE Hydrology Workshop SEIP program

NASA Technical Reports Server (NTRS)

2005-01-01

Matt Krigbaum (left), a teacher at Mitchell Elementary in Ann Arbor, Mich., pours water from the Pearl River into a turbidity tube to measure the river's light penetration. Krigbaum, along with Lois Williams, principal at Elizabeth Courville Elementary in Detroit, Mich.; and Carolyn Martin and Arlene Wittmer, teachers at Elizabeth Courville Elementary; conducted the experiment during a GLOBE (Global Learning and Observations to Benefit the Environment) hydrology workshop. GLOBE is a worldwide, hands-on science education program in which teachers can become certified to implement the program at their schools after taking hydrology, land cover/biology, atmosphere/climate and soil protocol workshops. Twelve teachers from across the country attended the recent weeklong GLOBE training at SSC, offered through its Educator Resource Center and the NASA Explorer Schools program. All workshops are free and offer continuing education units.

GLOBE Hydrology Workshop SEIP program

NASA Image and Video Library

2005-06-30

Matt Krigbaum (left), a teacher at Mitchell Elementary in Ann Arbor, Mich., pours water from the Pearl River into a turbidity tube to measure the river's light penetration. Krigbaum, along with Lois Williams, principal at Elizabeth Courville Elementary in Detroit, Mich.; and Carolyn Martin and Arlene Wittmer, teachers at Elizabeth Courville Elementary; conducted the experiment during a GLOBE (Global Learning and Observations to Benefit the Environment) hydrology workshop. GLOBE is a worldwide, hands-on science education program in which teachers can become certified to implement the program at their schools after taking hydrology, land cover/biology, atmosphere/climate and soil protocol workshops. Twelve teachers from across the country attended the recent weeklong GLOBE training at SSC, offered through its Educator Resource Center and the NASA Explorer Schools program. All workshops are free and offer continuing education units.

Pegasus XL CYGNSS Mission Science Briefing

NASA Image and Video Library

2016-12-10

In the Kennedy Space Center’s Press Site auditorium, NASA and industry leaders speak to members of the media during a mission science briefing for the agency’s Cyclone Global Navigation Satellite System, or CYGNSS, spacecraft. From left are: Aaron Ridley, CYGNSS constellation scientist in the Climate and Space Department at the University of Michigan in Ann Arbor, Michigan; and Mary Morris, doctoral student in the Department of Climate and Space Sciences and Engineering at the University of Michigan. The eight CYGNSS satellites will make frequent and accurate measurements of ocean surface winds throughout the life cycle of tropical storms and hurricanes. The data will help scientists probe key air-sea interaction processes that take place near the core of storms, which are rapidly changing and play a crucial role in the beginning and intensification of hurricanes.

Pegasus XL CYGNSS Mission Science Briefing

NASA Image and Video Library

2016-12-10

In the Kennedy Space Center’s Press Site auditorium, NASA and industry leaders speak to members of the media during a mission science briefing for the agency’s Cyclone Global Navigation Satellite System, or CYGNSS, spacecraft. From left are: Dr. Chris Ruf, CYGNSS principal investigator, Department of Climate and Space Sciences and Engineering at the University of Michigan; and Aaron Ridley, CYGNSS constellation scientist in the Climate and Space Department at the University of Michigan in Ann Arbor, Michigan. The eight CYGNSS satellites will make frequent and accurate measurements of ocean surface winds throughout the life cycle of tropical storms and hurricanes. The data will help scientists probe key air-sea interaction processes that take place near the core of storms, which are rapidly changing and play a crucial role in the beginning and intensification of hurricanes.

Biochemical and functional analysis of CTR1, a protein kinase that negatively regulates ethylene signaling in Arabidopsis

NASA Technical Reports Server (NTRS)

Huang, Yafan; Li, Hui; Hutchison, Claire E.; Laskey, James; Kieber, Joseph J.

2003-01-01

CTR1 encodes a negative regulator of the ethylene response pathway in Arabidopsis thaliana. The C-terminal domain of CTR1 is similar to the Raf family of protein kinases, but its first two-thirds encodes a novel protein domain. We used a variety of approaches to investigate the function of these two CTR1 domains. Recombinant CTR1 protein was purified from a baculoviral expression system, and shown to possess intrinsic Ser/Thr protein kinase activity with enzymatic properties similar to Raf-1. Deletion of the N-terminal domain did not elevate the kinase activity of CTR1, indicating that, at least in vitro, this domain does not autoinhibit kinase function. Molecular analysis of loss-of-function ctr1 alleles indicated that several mutations disrupt the kinase catalytic domain, and in vitro studies confirmed that at least one of these eliminates kinase activity, which indicates that kinase activity is required for CTR1 function. One missense mutation, ctr1-8, was found to result from an amino acid substitution within a new conserved motif within the N-terminal domain. Ctr1-8 has no detectable effect on the kinase activity of CTR1 in vitro, but rather disrupts the interaction with the ethylene receptor ETR1. This mutation also disrupts the dominant negative effect that results from overexpression of the CTR1 amino-terminal domain in transgenic Arabidopsis. These results suggest that CTR1 interacts with ETR1 in vivo, and that this association is required to turn off the ethylene-signaling pathway.

Copper homeostasis in grapevine: functional characterization of the Vitis vinifera copper transporter 1.

PubMed

Martins, Viviana; Bassil, Elias; Hanana, Mohsen; Blumwald, Eduardo; Gerós, Hernâni

2014-07-01

The Vitis vinifera copper transporter 1 is capable of self-interaction and mediates intracellular copper transport. An understanding of copper homeostasis in grapevine (Vitis vinifera L.) is particularly relevant to viticulture in which copper-based fungicides are intensively used. In the present study, the Vitis vinifera copper transporter 1 (VvCTr1), belonging to the Ctr family of copper transporters, was cloned and functionally characterized. Amino acid sequence analysis showed that VvCTr1 monomers are small peptides composed of 148 amino acids with 3 transmembrane domains and several amino acid residues typical of Ctr transporters. Bimolecular fluorescence complementation (BiFC) demonstrated that Ctr monomers are self-interacting and subcellular localization studies revealed that VvCTr1 is mobilized via the trans-Golgi network, through the pre-vacuolar compartment and located to the vacuolar membrane. The heterologous expression of VvCTr1 in a yeast strain lacking all Ctr transporters fully rescued the phenotype, while a deficient complementation was observed in a strain lacking only plasma membrane-bound Ctrs. Given the common subcellular localization of VvCTr1 and AtCOPT5 and the highest amino acid sequence similarity in comparison to the remaining AtCOPT proteins, Arabidopsis copt5 plants were stably transformed with VvCTr1. The impairment in root growth observed in copt5 seedlings in copper-deficient conditions was fully rescued by VvCTr1, further supporting its involvement in intracellular copper transport. Expression studies in V. vinifera showed that VvCTr1 is mostly expressed in the root system, but transcripts were also present in leaves and stems. The functional characterization of VvCTr-mediated copper transport provides the first step towards understanding the physiological and molecular responses of grapevines to copper-based fungicides.

An Ancestral Role for CONSTITUTIVE TRIPLE RESPONSE1 Proteins in Both Ethylene and Abscisic Acid Signaling1[OPEN

PubMed Central

Yasumura, Yuki; Pierik, Ronald; Kelly, Steven; Sakuta, Masaaki; Voesenek, Laurentius A.C.J.; Harberd, Nicholas P.

2015-01-01

Land plants have evolved adaptive regulatory mechanisms enabling the survival of environmental stresses associated with terrestrial life. Here, we focus on the evolution of the regulatory CONSTITUTIVE TRIPLE RESPONSE1 (CTR1) component of the ethylene signaling pathway that modulates stress-related changes in plant growth and development. First, we compare CTR1-like proteins from a bryophyte, Physcomitrella patens (representative of early divergent land plants), with those of more recently diverged lycophyte and angiosperm species (including Arabidopsis [Arabidopsis thaliana]) and identify a monophyletic CTR1 family. The fully sequenced P. patens genome encodes only a single member of this family (PpCTR1L). Next, we compare the functions of PpCTR1L with that of related angiosperm proteins. We show that, like angiosperm CTR1 proteins (e.g. AtCTR1 of Arabidopsis), PpCTR1L modulates downstream ethylene signaling via direct interaction with ethylene receptors. These functions, therefore, likely predate the divergence of the bryophytes from the land-plant lineage. However, we also show that PpCTR1L unexpectedly has dual functions and additionally modulates abscisic acid (ABA) signaling. In contrast, while AtCTR1 lacks detectable ABA signaling functions, Arabidopsis has during evolution acquired another homolog that is functionally distinct from AtCTR1. In conclusion, the roles of CTR1-related proteins appear to have functionally diversified during land-plant evolution, and angiosperm CTR1-related proteins appear to have lost an ancestral ABA signaling function. Our study provides new insights into how molecular events such as gene duplication and functional differentiation may have contributed to the adaptive evolution of regulatory mechanisms in plants. PMID:26243614

Efficacy of cold application on pain during chest tube removal: a randomized controlled trial: A CONSORT-compliant article.

PubMed

Hsieh, Ling-Yu; Chen, Yi-Rong; Lu, Mei-Chun

2017-11-01

Use of analgesics is the most common method to alleviate the pain induced by chest tube removal (CTR), but patient response to medication can vary and may not be achieved complete relaxation. This study was to determine the effectiveness of cold application in combination with standard analgesic administration before CTR on CTR-induced pain. A prospective, randomized, single-blind, sham-controlled study was conducted. In addition to the same routine care, subjects in the experimental group (n = 30) received cold application of 600-g ice packs 15 minutes before CTR, whereas subjects in the sham group (n = 30) received tap water packs. Numerical rating scale was used to measure pain intensity before, immediately after, and 10 minutes after CTR. The generalized linear estimating equation (GEE) model, adjusted for other factors, both the groups demonstrated a trend toward decreased pain during CTR over time (P < .001), but no significant differences between the 2 groups (P = .65), even stratifying by gender. If we fixed experimental group, women significant reduced pain score of 2.7 on immediately after CTR compared with before CTR (P < .0001) and reduced pain score of 2.05 on 10 minutes after CTR compared with before CTR (P < .0001). The sham group had no similar performance as the experimental group. In the male subgroup, both experimental and sham groups, men significantly reduced pain score on immediately after CTR and 10 minutes after CTR compared with before CTR (P < .0001). The results indicate that cold application is not more effective than sham treatment in decreasing pain during CTR, even among gender. Although statistically non-significant, clinically important differences of decreased pain score were observed with cold application among women (Clinical Trial Registration: clinicaltrials.gov identifier NCT03307239).

Conference Comments by the Editors

DOE Office of Scientific and Technical Information (OSTI.GOV)

Archer, Daniel E

2009-01-01

The Symposium on Radiation Measurements and Applications (SORMA) met for the first time on the West Coast June 2-5, 2008, in Berkeley, CA. With radiation detectors increasing in number, variety, and societal importance, we plan to alternate between SORMA East (in Ann Arbor, MI) and SORMA West so that the forum will be available every two years. The topic areas for SORMA West 2008 were much the same as those of recent Ann Arbor programs, and were meant to encompass the full breadth of ionizing radiation measurement applications and technologies, with both oral and poster presentations. The technical program ofmore » SORMA 2008 included 342 scientific presentations, 116 oral presentations plus eight invited keynote lectures in plenary sessions, as well as 218 presentations in poster sessions. The SORMA 2008 meetings were attended by 439 registered participants from 25 different countries. Topics of interest at the meeting ranged from room temperature semiconductor detectors, cryogenic detectors, photodetectors, neutron detectors, novel scintillators, nonproportionality and characterization of scintillators, simulation and analysis of radiation interactions, novel radiation sources, imaging technologies and homeland security and medical applications. This was the first conference of the SORMA series to be technically cosponsored by the Institute of Electrical and Electronic Engineers (IEEE), specifically by the Nuclear and Plasma Sciences Society (NPSS) of IEEE. The co-sponsorship has been important for visibility of SORMA and for attracting a record number of new participants. This special issue of IEEE TRANSACTIONS ON NUCLEAR SCIENCE comprises the refereed proceedings of SORMA 2008, containing 128 papers on the research presentations. The Chairs of the SORMA 2008 conference would like to acknowledge partial financial support from the NNSA, DNDO, and DTRA, and organizational support from Lawrence Berkeley National Laboratory and Lawrence Livermore National Laboratory. We acknowledge our corporate supporters: Caen Nuclear, Eljen Technology, Hilger Crystals and GE Global Research. Finally, we thank the members of the local organizing committee: Diana Attila, Thomas Budinger, Joe Chew, Daniel Chivers, Rob Johnson, Laurie O'Brien, Donna Raziano, Emily Sause, and Brian Wirth for doing all the work that actually made this conference happen.« less

Evaluating effects of land pattern with regional characteristic curves: Land cover indicators and estimating building energy use from thermal image data

NASA Astrophysics Data System (ADS)

Lewis, Geoffrey Mcdowell

Sustainability is a word that represents a wide spectrum of complex and interrelated topics, but it is fundamentally a concept that describes a balanced relationship with the physical environment. There is a need for timely feedback on that relationship in order for individuals and societies to make informed decisions. Investigating the relationship between the physical form of our cities and regions and the impacts that these regions have on their supporting ecological environment requires both a method for categorizing form and a method for quantifying impact. The latter was addressed through the development of the regional characteristic curve method. This method collects, summarizes, analyzes, and displays spatial data in a graphical form that is intuitive, informative, and has advantages over existing tabular methods. Regional characteristic curves are calculated using concentric rings as the basis for evaluating impact metrics from any kind of areal data. The method was investigated in an examination of spatial change over time in the Ann Arbor region and in a comparison between Ann Arbor and Detroit. Curves for several types of metrics were used, based on land-cover and demographic data. The lack of a suitable energy use data set was highlighted, as well as great impact that energy use causes. One aspect of energy use, energy use in buildings, was singled out for further investigation. The possible link between building energy use and thermal image data collected by satellite was explored. Satellite thermal image data has been shown to be primarily related to variation in land-cover, but remaining variability in satellite data may be related to energy use in buildings. This link was investigated in both summer and winter case studies, revealing no significant relationship in summer and a weak relationship in winter. The winter relationship was also in a counterintuitive direction, in that higher building energy use was associated with cooler thermal image data. The poor spatial resolution and timing of the satellite data collection were not advantageous, so the presence of even a weak and counterintuitive result encouraged further exploration of this topic.

[Structure-functional organization of eukaryotic high-affinity copper importer CTR1 determines its ability to transport copper, silver and cisplatin].

PubMed

Skvortsov, A N; Zatulovskiĭ, E A; Puchkova, L V

2012-01-01

It was shown recently, that high affinity Cu(I) importer eukaryotic protein CTR1 can also transport in vitro abiogenic Ag(I) ions and anticancer drug cisplatin. At present there is no rational explanation how CTR1 can transfer platinum group, which is different by coordination properties from highly similar Cu(I) and Ag(I). To understand this phenomenon we analyzed 25 sequences of chordate CTR1 proteins, and found out conserved patterns of organization of N-terminal extracellular part of CTR1 which correspond to initial metal binding. Extracellular copper-binding motifs were qualified by their coordination properties. It was shown that relative position of Met- and His-rich copper-binding motifs in CTR1 predisposes the extracellular CTR1 part to binding of copper, silver and cisplatin. Relation between tissue-specific expression of CTR1 gene, steady-state copper concentration, and silver and platinum accumulation in organs of mice in vivo was analyzed. Significant positive but incomplete correlation exists between these variables. Basing on structural and functional peculiarities of N-terminal part of CTR1 a hypothesis of coupled transport of copper and cisplatin has been suggested, which avoids the disagreement between CTR1-mediated cisplatin transport in vitro, and irreversible binding of platinum to Met-rich peptides.

Accumulation of Ag and Cu in Amanita strobiliformis and characterization of its Cu and Ag uptake transporter genes AsCTR2 and AsCTR3.

PubMed

Beneš, Vojtěch; Hložková, Kateřina; Matěnová, Michaela; Borovička, Jan; Kotrba, Pavel

2016-04-01

Macrofungi can accumulate in their sporocarps remarkably high concentrations of Cu and Ag. We have previously demonstrated that the non-essential Ag is in the ectomycorrhizal, Ag-hyperaccumulating Amanita strobiliformis sequestered by 3.4-kDa metallothioneins (MTs) produced as AsMT1a, 1b and 1c isoforms. Here, we describe two populations of wild-grown A. strobiliformis sporocarps, which showed certain correlation between the concentrations of accumulated Ag (284 ± 64 and 67 ± 15 mg kg(-1)) and Cu (76 ± 13 and 30 ± 12 mg kg(-1)), suggesting that an overlap may exist in the cell biology of Ag and Cu in this species. Metal speciation analysis revealed that the intracellular Cu in the sporocarps of both populations was, like Ag, associated with the 3.4-kDa MTs. A search of A. strobiliformis transcriptome for sequences encoding proteins of the Cu transporter (CTR) family identified four AsCTR cDNAs, which were, like AsMT1s, confirmed in both populations. The predicted AsCTR proteins showed homology to vacuolar (AsCTR1 and AsCTR4) and plasma membrane (AsCTR2 and AsCTR3) CTRs. Heterologous expression of AsCTR2, AsCTR3 and their translational fusions with green fluorescent protein (GFP) in Cu uptake-deficient S. cerevisiae indicated that both AsCTRs are functional Cu and Ag uptake transporters: recombinant genes complemented growth defects and increased Cu and Ag uptake rates in yeasts and the GFP-tagged protein localized to the cell periphery. Site directed mutagenesis revealed the importance of the conserved-among-CTRs M-X3-M motif for the AsCTR2- and AsCTR3-mediated transport of both Cu and Ag. These results provide the first evidence that fungal CTRs can recognize Ag for transport.

Rate and Regulation of Copper Transport by Human Copper Transporter 1 (hCTR1)*

PubMed Central

Maryon, Edward B.; Molloy, Shannon A.; Ivy, Kristin; Yu, Huijun; Kaplan, Jack H.

2013-01-01

Human copper transporter 1 (hCTR1) is a homotrimer of a 190-amino acid monomer having three transmembrane domains believed to form a pore for copper permeation through the plasma membrane. The hCTR1-mediated copper transport mechanism is not well understood, nor has any measurement been made of the rate at which copper ions are transported by hCTR1. In this study, we estimated the rate of copper transport by the hCTR1 trimer in cultured cells using 64Cu uptake assays and quantification of plasma membrane hCTR1. For endogenous hCTR1, we estimated a turnover number of about 10 ions/trimer/s. When overexpressed in HEK293 cells, a second transmembrane domain mutant of hCTR1 (H139R) had a 3-fold higher Km value and a 4-fold higher turnover number than WT. Truncations of the intracellular C-terminal tail and an AAA substitution of the putative metal-binding HCH C-terminal tripeptide (thought to be required for transport) also exhibited elevated transport rates and Km values when compared with WT hCTR1. Unlike WT hCTR1, H139R and the C-terminal mutants did not undergo regulatory endocytosis in elevated copper. hCTR1 mutants combining methionine substitutions that block transport (M150L,M154L) on the extracellular side of the pore and the high transport H139R or AAA intracellular side mutations exhibited the blocked transport of M150L,M154L, confirming that Cu+ first interacts with the methionines during permeation. Our results show that hCTR1 elements on the intracellular side of the hCTR1 pore, including the carboxyl tail, are not essential for permeation, but serve to regulate the rate of copper entry. PMID:23658018

Primary follicular lymphoma of the cervix uteri: a review.

PubMed

Korcum, Aylin Fidan; Karadogan, Ihsan; Aksu, Gamze; Aralasmak, Ayse; Erdogan, Gulgun

2007-09-01

Primary non-Hodgkin's lymphoma of the cervix is a rare disease, of which a subgroup of follicular lymphoma constitutes only 8.5%. There is not an established treatment protocol neither for primary cervical lymphoma nor for its follicular subgroup. We presented a case with Ann Arbor stage IEA (Extra-nodal involvement and absence of weight loss, fever, night sweat) primary follicular lymphoma of the cervix. She was treated with chemotherapy followed by pelvic radiotherapy. Upon relapse with a nodal neck mass, she was treated with rituximab alone. She remained well for 23 months after rituximab. In the 39 months of follow-up, there was no evidence of disease. In the light of our case, we reviewed the reported cases of primary follicular lymphoma of the cervix while discussing their treatment protocols and the cases of primary cervix lymphoma treated with rituximab.

Rebuilding astronomy at Michigan: from Hussey to Goldberg

NASA Astrophysics Data System (ADS)

Lindner, Rudi Paul

2003-12-01

The University of Michigan astronomy programme, in research and teaching, was in terrible shape when W.J. Hussey returned to revive it in 1905. With support from the administration and an old friend, Hussey built a new, astrophysical observatory and planned a southern station to pursue his double star campaign. His successor, Ralph Hamilton Curtiss, developed a school of astronomical spectroscopy and saw the southern station, the Lamont-Hussey Observatory, in full operation. After Ralph Curtiss' early death, Heber Curtis continued, nurtured the McMath-Hulbert Observatory and wedded it to the Unoversity, and obtained the pyrex disk for a 2.49-m (98-inch) reflector. The Great Depression deprived the Ann Arbor programme of its momentum, but after World War II a new Director, Leo Goldberg, made the Department a formidable presence in American astronomical research and training.

AORN and University of Michigan School of nursing research alliance.

PubMed

Talsma, Akkeneel; Chard, Robin; Kleiner, Catherine; Anderson, Christine; Geun, Hyogeun

2011-06-01

Research related to perioperative care requires advanced training and is well suited to take place at a research-intensive university. A recent research alliance established between AORN and the University of Michigan School of Nursing, Ann Arbor, uses the strengths of both a robust perioperative professional organization and a research-intensive university to make progress toward improving patient safety and transforming the perioperative work environment. Research activities undertaken by this alliance include investigating nurse staffing characteristics and patient outcomes, as well as evaluating the congruence and definitions of data elements contained in AORN's SYNTEGRITY™ Standardized Perioperative Framework. Disseminating the findings of the alliance is expected to facilitate the communication and application of new knowledge to nursing practice and help advance the perioperative nursing profession. Copyright © 2011 AORN, Inc. Published by Elsevier Inc. All rights reserved.

Reduced 64Cu uptake and tumor growth inhibition by knockdown of human copper transporter 1 in xenograft mouse model of prostate cancer.

PubMed

Cai, Huawei; Wu, Jiu-sheng; Muzik, Otto; Hsieh, Jer-Tsong; Lee, Robert J; Peng, Fangyu

2014-04-01

Copper is an element required for cell proliferation and angiogenesis. Human prostate cancer xenografts with increased (64)Cu radioactivity were visualized previously by PET using (64)CuCl2 as a radiotracer ((64)CuCl2 PET). This study aimed to determine whether the increased tumor (64)Cu radioactivity was due to increased cellular uptake of (64)Cu mediated by human copper transporter 1 (hCtr1) or simply due to nonspecific binding of ionic (64)CuCl2 to tumor tissue. In addition, the functional role of hCtr1 in proliferation of prostate cancer cells and tumor growth was also assessed. A lentiviral vector encoding short-hairpin RNA specific for hCtr1 (Lenti-hCtr1-shRNA) was constructed for RNA interference-mediated knockdown of hCtr1 expression in prostate cancer cells. The degree of hCtr1 knockdown was determined by Western blot, and the effect of hCtr1 knockdown on copper uptake and proliferation were examined in vitro by cellular (64)Cu uptake and cell proliferation assays. The effects of hCtr1 knockdown on tumor uptake of (64)Cu were determined by PET quantification and tissue radioactivity assay. The effects of hCtr1 knockdown on tumor growth were assessed by PET/CT and tumor size measurement with a caliper. RNA interference-mediated knockdown of hCtr1 was associated with the reduced cellular uptake of (64)Cu and the suppression of prostate cancer cell proliferation in vitro. At 24 h after intravenous injection of the tracer (64)CuCl2, the (64)Cu uptake by the tumors with knockdown of hCtr1 (4.02 ± 0.31 percentage injected dose per gram [%ID/g] in Lenti-hCtr1-shRNA-PC-3 and 2.30 ± 0.59 %ID/g in Lenti-hCtr1-shRNA-DU-145) was significantly lower than the (64)Cu uptake by the control tumors without knockdown of hCtr1 (7.21 ± 1.48 %ID/g in Lenti-SCR-shRNA-PC-3 and 5.57 ± 1.20 %ID/g in Lenti-SCR-shRNA-DU-145, P < 0.001) by PET quantification. Moreover, the volumes of prostate cancer xenograft tumors with knockdown of hCtr1 (179 ± 111 mm(3) for Lenti-hCtr1-shRNA-PC-3 or 39 ± 22 mm(3) for Lenti-hCtr1-shRNA-DU-145) were significantly smaller than those without knockdown of hCtr1 (536 ± 191 mm(3) for Lenti- SCR-shRNA-PC-3 or 208 ± 104 mm(3) for Lenti-SCR-shRNA-DU-145, P < 0.01). Overall, data indicated that hCtr1 is a promising theranostic target, which can be further developed for metabolic imaging of prostate cancer using (64)CuCl2 PET/CT and personalized cancer therapy targeting copper metabolism.

The N-terminus of the human copper transporter 1 (hCTR1) is localized extracellularly, and interacts with itself.

PubMed Central

Klomp, Adriana E M; Juijn, Jenneke A; van der Gun, Linda T M; van den Berg, Inge E T; Berger, Ruud; Klomp, Leo W J

2003-01-01

We have used indirect immunofluorescense studies and glycosylation-site insertion and deletion mapping to characterize the topology of human copper transporter 1 (hCTR1), the putative human high-affinity copper-import protein. Both approaches indicated that hCTR1 contains three transmembrane domains and that the N-terminus of hCTR1, which contains several putative copper-binding sites, is localized extracellularly, whereas the C-terminus is exposed to the cytosol. Based on previous observations that CTR1 proteins form high-molecular-mass complexes, we investigated directly whether CTR1 proteins interact with themselves. Yeast two-hybrid studies showed that interaction of yeast, mouse, rat and human CTR1 occurs at the sites of their N-terminal domains, and is not dependent on the copper concentration in the growth media. Analysis of deletion constructs indicated that multiple regions in the N-terminus are essential for this self-interaction. In contrast, the N-terminal tail of the presumed low-affinity copper transporter, hCTR2, does not interact with itself. Taken together, these results suggest that CTR1 spans the membrane at least six times, permitting formation of a channel, which is consistent with its proposed role as a copper transporter. PMID:12466020

Ctr9, a Protein in the Transcription Complex Paf1, Regulates Dopamine Transporter Activity at the Plasma Membrane.

PubMed

De Gois, Stéphanie; Slama, Patrick; Pietrancosta, Nicolas; Erdozain, Amaia M; Louis, Franck; Bouvrais-Veret, Caroline; Daviet, Laurent; Giros, Bruno

2015-07-17

Dopamine (DA) is a major regulator of sensorimotor and cognitive functions. The DA transporter (DAT) is the key protein that regulates the spatial and temporal activity of DA release into the synaptic cleft via the rapid reuptake of DA into presynaptic termini. Several lines of evidence have suggested that transporter-interacting proteins may play a role in DAT function and regulation. Here, we identified the tetratricopeptide repeat domain-containing protein Ctr9 as a novel DAT binding partner using a yeast two-hybrid system. We showed that Ctr9 is expressed in dopaminergic neurons and forms a stable complex with DAT in vivo via GST pulldown and co-immunoprecipitation assays. In mammalian cells co-expressing both proteins, Ctr9 partially colocalizes with DAT at the plasma membrane. This interaction between DAT and Ctr9 results in a dramatic enhancement of DAT-mediated DA uptake due to an increased number of DAT transporters at the plasma membrane. We determined that the binding of Ctr9 to DAT requires residues YKF in the first half of the DAT C terminus. In addition, we characterized Ctr9, providing new insight into this protein. Using three-dimensional modeling, we identified three novel tetratricopeptide repeat domains in the Ctr9 sequence, and based on deletion mutation experiments, we demonstrated the role of the SH2 domain of Ctr9 in nuclear localization. Our results demonstrate that Ctr9 localization is not restricted to the nucleus, as previously described for the transcription complex Paf1. Taken together, our data provide evidence that Ctr9 modulates DAT function by regulating its trafficking. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

LeCTR2, a CTR1-like protein kinase from tomato, plays a role in ethylene signalling, development and defence

PubMed Central

Lin, Zhefeng; Alexander, Lucy; Hackett, Rachel; Grierson, Don

2008-01-01

Arabidopsis AtCTR1 is a Raf-like protein kinase that interacts with ETR1 and ERS and negatively regulates ethylene responses. In tomato, several CTR1-like proteins could perform this role. We have characterized LeCTR2, which has similarity to AtCTR1 and also to EDR1, a CTR1-like Arabidopsis protein involved in defence and stress responses. Protein–protein interactions between LeCTR2 and six tomato ethylene receptors indicated that LeCTR2 interacts preferentially with the subfamily I ETR1-type ethylene receptors LeETR1 and LeETR2, but not the NR receptor or the subfamily II receptors LeETR4, LeETR5 and LeETR6. The C-terminus of LeCTR2 possesses serine/threonine kinase activity and is capable of auto-phosphorylation and phosphorylation of myelin basic protein in vitro. Overexpression of the LeCTR2 N-terminus in tomato resulted in altered growth habit, including reduced stature, loss of apical dominance, highly branched inflorescences and fruit trusses, indeterminate shoots in place of determinate flowers, and prolific adventitious shoot development from the rachis or rachillae of the leaves. Expression of the ethylene-responsive genes E4 and chitinase B was upregulated in transgenic plants, but ethylene production and the level of mRNA for the ethylene biosynthetic gene ACO1 was unaffected. The leaves and fruit of transgenic plants also displayed enhanced susceptibility to infection by the fungal pathogen Botrytis cinerea, which was associated with much stronger induction of pathogenesis-related genes such as PR1b1 and chitinase B compared with the wild-type. The results suggest that LeCTR2 plays a role in ethylene signalling, development and defence, probably through its interactions with the ETR1-type ethylene receptors of subfamily I. PMID:18346193

Relative contribution of CTR1 and DMT1 in copper transport by the blood–CSF barrier: Implication in manganese-induced neurotoxicity

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zheng, Gang; Department of Occupational and Environmental Health and the Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Fourth Military Medical University, Xi'an, Shanxi 710032; Chen, Jingyuan

2012-05-01

The homeostasis of copper (Cu) in the cerebrospinal fluid (CSF) is partially regulated by the Cu transporter-1 (CTR1) and divalent metal transporter-1 (DMT1) at the blood–CSF barrier (BCB) in the choroid plexus. Data from human and animal studies suggest an increased Cu concentration in blood, CSF, and brains following in vivo manganese (Mn) exposure. This study was designed to investigate the relative role of CTR1 and DMT1 in Cu transport under normal or Mn-exposed conditions using an immortalized choroidal Z310 cell line. Mn exposure in vitro resulted in an increased cellular {sup 64}Cu uptake and the up-regulation of both CTR1more » and DMT1. Knocking down CTR1 by siRNA counteracted the Mn-induced increase of {sup 64}Cu uptake, while knocking down DMT1 siRNA resulted in an increased cellular {sup 64}Cu uptake in Mn-exposed cells. To distinguish the roles of CTR1 and DMT1 in Cu transport, the Z310 cell-based tetracycline (Tet)-inducible CTR1 and DMT1 expression cell lines were developed, namely iZCTR1 and iZDMT1 cells, respectively. In iZCTR1 cells, Tet induction led to a robust increase (25 fold) of {sup 64}Cu uptake with the time course corresponding to the increased CTR1. Induction of DMT1 by Tet in iZDMT1 cells, however, resulted in only a slight increase of {sup 64}Cu uptake in contrast to a substantial increase in DMT1 mRNA and protein expression. These data indicate that CTR1, but not DMT1, plays an essential role in transporting Cu by the BCB in the choroid plexus. Mn-induced cellular overload of Cu at the BCB is due, primarily, to Mn-induced over-expression of CTR1. -- Highlights: ► This study compares the relative role of CTR1 and DMT1 in Cu transport by the BCB. ► Two novel tetracycline-inducible CTR1 and DMT1 expression cell lines are created. ► CTR1, but not DMT1, plays an essential role in transporting Cu by the BCB. ► Mn-induced cellular Cu overload is due to its induction of CTR1 rather than DMT1. ► Induction of CTR1 by Mn in the BCB contributes to an elevated Cu level in the CSF.« less

Implementation of Prolonged Exposure in the Army: Is Consultation Necessary for Effective Dissemination

DTIC Science & Technology

2014-10-01

University of Pennsylvania, Allison Chernov, was trained to assist with the study at this time. In September 2013, Sally Curtis and Kristen Butcher were...Ronald L CTR USARMY MEDCOM Hoover (US) <ronald.l.hoover.ctr@mail.mil>, Inna R CTR USARMY MEDCOM CDMRP Williams (US) <inna.r.williams.ctr@mail.mil

Cyber Operational Architecture Training System Cyber for All

DTIC Science & Technology

2015-12-30

Ingenia Services, Inc. Camp H.M. Smith , HI Camp H.M. Smith , HI william.d.wells1.ctr@pacom.mil derek.bryan.ctr@pacom.mil ABSTRACT Current...War Innovation Center USPACOM J81 / Ingenia Services, Inc. Camp H.M. Smith , HI Camp H.M. Smith , HI william.d.wells1.ctr@pacom.mil derek.bryan.ctr

Annual Research Briefs, 1990

NASA Technical Reports Server (NTRS)

1991-01-01

The 1990 annual progress reports of the Research Fellows and students of the Center for Turbulent Research (CTR) are included. It is intended primarily as a contractor report to NASA, Ames Research Center. In addition, numerous CTR Manuscript Reports were published last year. The purpose of the CTR Manuscript Series is to expedite the dissemination of research results by the CTR staff. The CTR is devoted to the fundamental study of turbulent flow; its objectives are to produce advances in physical understanding of turbulence, in turbulence modeling and simulation, and in turbulence control.

Lessons for tumor biomarker trials: vicious cycles, scientific method & developing guidelines.

PubMed

Hayes, Daniel; Raison, Claire

2015-02-01

Interview with Daniel Hayes, by Claire Raison (Commissioning Editor) Daniel F Hayes, M.D. is the Stuart A Padnos Professor of Breast Cancer Research and co-Director of the Breast Oncology Program at the University of Michigan Comprehensive Cancer Center (Ann Arbor, MI, USA). Dr Hayes has extensive experience in clinical and translational breast cancer biomarker research, and in drug development and clinical trials. Around 30 years ago, he led the discovery of the circulating breast tumor biomarker, CA15-3, which started his career into further tumor biomarker work. The main thrust of his work since then has been in clinical trials, tumor biomarkers and trying to integrate the two. Dr Hayes is Chair of the Correlative Sciences Committee of the North American Breast Cancer Group (now called the Breast Cancer Steering Committee), and co-chairs the Expert Panel for Tumor Biomarker Practice Guidelines for the American Society of Clinical Oncology.

Eighth International Chorea-Acanthocytosis Symposium: Summary of Workshop Discussion and Action Points.

PubMed

Pappas, Samuel S; Bonifacino, Juan; Danek, Adrian; Dauer, William T; De, Mithu; De Franceschi, Lucia; DiPaolo, Gilbert; Fuller, Robert; Haucke, Volker; Hermann, Andreas; Kornmann, Benoit; Landwehrmeyer, Bernhard; Levin, Johannes; Neiman, Aaron M; Rudnicki, Dobrila D; Sibon, Ody; Velayos-Baeza, Antonio; Vonk, Jan J; Walker, Ruth H; Weisman, Lois S; Albin, Roger L

2017-01-01

Chorea-Acanthocytosis (ChAc) is a rare hereditary neurological disorder characterized by abnormal movements, red blood cell pathology, and progressive neurodegeneration. Little is understood of the pathogenesis of ChAc and related disorders (collectively Neuroacanthocytosis). The Eighth International Chorea-Acanthocytosis Symposium was held in May 2016 in Ann Arbor, MI, USA, and focused on molecular mechanisms driving ChAc pathophysiology. Accompanying the meeting, members of the neuroacanthocytosis research community and other invited scientists met in a workshop to discuss the current understanding and next steps needed to better understand ChAc pathogenesis. These discussions identified several broad and critical needs for advancing ChAc research and patient care, and led to the definition of 18 specific action points related to functional and molecular studies, animal models, and clinical research. These action points, described below, represent tractable research goals to pursue for the next several years.

Breast Implant-Associated Anaplastic Large Cell Lymphoma: Case Report and Review of the Literature.

PubMed

Berlin, Eva; Singh, Kunwar; Mills, Christopher; Shapira, Ilan; Bakst, Richard L; Chadha, Manjeet

2018-01-01

We are reporting the case of a 58-year-old woman with history of bilateral silicone breast implants for cosmetic augmentation. At 2-year interval from receiving the breast implants, she presented with swelling of the right breast with associated chest wall mass, effusion around the implant, and axillary lymphadenopathy. Pathology confirmed breast implant-associated anaplastic large cell lymphoma (stage III, T4N2M0, using BIA-ALCL TNM staging and stage IIAE, using Ann-Arbor staging). The patient underwent bilateral capsulectomy and right partial mastectomy with excision of the right breast mass and received adjuvant CHOP chemotherapy and radiation to the right breast and regional nodes. Since completion of multimodality therapy, the patient has sustained remission on both clinical exam and PET/CT scan. We report this case and review of the literature on this rare form of lymphoma.

Breast Implant-Associated Anaplastic Large Cell Lymphoma: Case Report and Review of the Literature

PubMed Central

Singh, Kunwar; Mills, Christopher; Shapira, Ilan

2018-01-01

We are reporting the case of a 58-year-old woman with history of bilateral silicone breast implants for cosmetic augmentation. At 2-year interval from receiving the breast implants, she presented with swelling of the right breast with associated chest wall mass, effusion around the implant, and axillary lymphadenopathy. Pathology confirmed breast implant-associated anaplastic large cell lymphoma (stage III, T4N2M0, using BIA-ALCL TNM staging and stage IIAE, using Ann-Arbor staging). The patient underwent bilateral capsulectomy and right partial mastectomy with excision of the right breast mass and received adjuvant CHOP chemotherapy and radiation to the right breast and regional nodes. Since completion of multimodality therapy, the patient has sustained remission on both clinical exam and PET/CT scan. We report this case and review of the literature on this rare form of lymphoma. PMID:29607225

Pegasus XL CYGNSS Mission Science Briefing

NASA Image and Video Library

2016-12-10

In the Kennedy Space Center’s Press Site auditorium, NASA and industry leaders speak to members of the media during a mission science briefing for the agency’s Cyclone Global Navigation Satellite System, or CYGNSS, spacecraft. From left are: Sean Potter of NASA Communications; Dr. Chris Ruf, CYGNSS principal investigator, Department of Climate and Space Sciences and Engineering at the University of Michigan; Aaron Ridley, CYGNSS constellation scientist in the Climate and Space Department at the University of Michigan in Ann Arbor, Michigan; and Mary Morris, doctoral student in the Department of Climate and Space Sciences and Engineering at the University of Michigan. The eight CYGNSS satellites will make frequent and accurate measurements of ocean surface winds throughout the life cycle of tropical storms and hurricanes. The data will help scientists probe key air-sea interaction processes that take place near the core of storms, which are rapidly changing and play a crucial role in the beginning and intensification of hurricanes.

Pegasus XL CYGNSS Mission Science Briefing

NASA Image and Video Library

2016-12-10

In the Kennedy Space Center’s Press Site auditorium, NASA and industry leaders speak to members of the media during a mission science briefing for the agency’s Cyclone Global Navigation Satellite System, or CYGNSS, spacecraft. From left are: Dr. Chris Ruf, CYGNSS principal investigator, Department of Climate and Space Sciences and Engineering at the University of Michigan; Aaron Ridley, CYGNSS constellation scientist in the Climate and Space Department at the University of Michigan in Ann Arbor, Michigan; and Mary Morris, doctoral student in the Department of Climate and Space Sciences and Engineering at the University of Michigan. The eight CYGNSS satellites will make frequent and accurate measurements of ocean surface winds throughout the life cycle of tropical storms and hurricanes. The data will help scientists probe key air-sea interaction processes that take place near the core of storms, which are rapidly changing and play a crucial role in the beginning and intensification of hurricanes.

Measurement of solar radiation at the Earth's surface

NASA Technical Reports Server (NTRS)

Bartman, F. L.

1982-01-01

The characteristics of solar energy arriving at the surface of the Earth are defined and the history of solar measurements in the United States presented. Radiation and meteorological measurements being made at solar energy meteorological research and training sites and calibration procedures used there are outlined. Data illustrating the annual variation in daily solar radiation at Ann Arbor, Michigan and the diurnal variation in radiation at Albuquerque, New Mexico are presented. Direct normal solar radiation received at Albuquerque is contrasted with that received at Maynard, Massachusetts. Average measured global radiation for a period of one year for four locations under clear skies, 50% cloud cover, and 100% cloud cover is given and compared with the solar radiation at the top of the atmosphere. The May distribution of mean daily direct solar radiation and mean daily global solar radiation over the United States is presented. The effects of turbidity on the direct and circumsolar radiation are shown.

Strategic planning as a tool for achieving alignment in academic health centers.

PubMed

Higginbotham, Eve J; Church, Kathryn C

2012-01-01

After the passage of the Patient Protection and Affordable Care Act in March 2010, there is an urgent need for medical schools, teaching hospitals, and practice plans to work together seamlessly across a common mission. Although there is agreement that there should be greater coordination of initiatives and resources, there is little guidance in the literature to address the method to achieve the necessary transformation. Traditional approaches to strategic planning often engage a few leaders and produce a set of immeasurable initiatives. A nontraditional approach, consisting of a Whole-Scale (Dannemiller Tyson Associates, Ann Arbor, MI) engagement, appreciative inquiry, and a balanced scorecard can, more rapidly transform an academic health center. Using this nontraditional approach to strategic planning, increased organizational awareness was achieved in a single academic health center. Strategic planning can be an effective tool to achieve alignment, enhance accountability, and a first step in meeting the demands of the new landscape of healthcare.

Ranging algebraically with more observations than unknowns

NASA Astrophysics Data System (ADS)

Awange, J. L.; Fukuda, Y.; Takemoto, S.; Ateya, I. L.; Grafarend, E. W.

2003-07-01

In the recently developed Spatial Reference System that is designed to check and control the accuracy of the three-dimensional coordinate measuring machines and tooling equipment (Metronom US., Inc., Ann Arbor: http://www.metronomus.com), the coordinates of the edges of the instrument are computed from distances of the bars. The use of distances in industrial application is fast gaining momentum just as in Geodesy and in Geophysical applications and thus necessitating efficient algorithms to solve the nonlinear distance equations. Whereas the ranging problem with minimum known stations was considered in our previous contribution in the same Journal, the present contribution extends to the case where one is faced with many distance observations than unknowns (overdetermined case) as is usually the case in practise. Using the Gauss-Jacobi Combinatorial approach, we demonstrate how one can proceed to position without reverting to iterative and linearizing procedures such as Newton's or Least Squares approach.

International Symposium on Remote Sensing of Environment, 9th, University of Michigan, Ann Arbor, Mich., April 15-19, 1974, Proceedings. Volumes 1, 2 & 3

NASA Technical Reports Server (NTRS)

1974-01-01

The present work gathers together numerous papers describing the use of remote sensing technology for mapping, monitoring, and management of earth resources and man's environment. Studies using various types of sensing equipment are described, including multispectral scanners, radar imagery, spectrometers, lidar, and aerial photography, and both manual and computer-aided data processing techniques are described. Some of the topics covered include: estimation of population density in Tokyo districts from ERTS-1 data, a clustering algorithm for unsupervised crop classification, passive microwave sensing of moist soils, interactive computer processing for land use planning, the use of remote sensing to delineate floodplains, moisture detection from Skylab, scanning thermal plumes, electrically scanning microwave radiometers, oil slick detection by X-band synthetic aperture radar, and the use of space photos for search of oil and gas fields. Individual items are announced in this issue.

Laboratory-produced ball lightning

NASA Astrophysics Data System (ADS)

Golka, Robert K., Jr.

1994-05-01

For 25 years I have actively been searching for the true nature of ball lightning and attempting to reproduce it at will in the laboratory. As one might expect, many unidentified lights in the atmosphere have been called ball lightning, including Texas Maffa lights (automobile headlights), flying saucers (UFOs), swamp gas in Ann Arbor, Michigan, etc. For 15 years I thought ball lightning was strictly a high-voltage phenomenon. It was not until 1984 when I was short-circuiting the electrical output of a diesel electric railroad locomotive that I realized that the phenomenon was related more to a high current. Although I am hoping for some other types of ball lightning to emerge such as strictly electrostatic-electromagnetic manifestations, I have been unlucky in finding laboratory provable evidence. Cavity-formed plasmodes can be made by putting a 2-inch burning candle in a home kitchen microwave oven. The plasmodes float around for as long as the microwave energy is present.

Research productivity in select psychology journals, 1986-2008.

PubMed

Mahoney, Kevin T; Buboltz, Walter C; Calvert, Barbara; Hoffmann, Rebecca

2010-01-01

Examination of research productivity has a long history in psychology. Journals across psychology have periodically published research-productivity studies. An analysis of institutional research productivity was conducted for 17 journals published by the American Psychological Association for the years 1986-2008. This analysis implemented two methodologies: one a replication and extension of G. S. Howard, D. A. Cole, and S. E. Maxwell's (1987) method, the other a new method designed to give credit to psychology departments rather than only overall institutions. A system of proportional credit assured all articles with multiple institutions received credit. Results show that for the 23-year period, the University of Illinois at Urbana-Champaign was ranked 1st, followed by the University of California, Los Angeles, and the University of Michigan, Ann Arbor. Overall, results showed both consistency and change across all journals examined. The authors explore the implications of these findings in the context of the current academic environment.

An sRNA and Cold Shock Protein Homolog-Based Feedforward Loop Post-transcriptionally Controls Cell Cycle Master Regulator CtrA

PubMed Central

Robledo, Marta; Schlüter, Jan-Philip; Loehr, Lars O.; Linne, Uwe; Albaum, Stefan P.; Jiménez-Zurdo, José I.; Becker, Anke

2018-01-01

Adjustment of cell cycle progression is crucial for bacterial survival and adaptation under adverse conditions. However, the understanding of modulation of cell cycle control in response to environmental changes is rather incomplete. In α-proteobacteria, the broadly conserved cell cycle master regulator CtrA underlies multiple levels of control, including coupling of cell cycle and cell differentiation. CtrA levels are known to be tightly controlled through diverse transcriptional and post-translational mechanisms. Here, small RNA (sRNA)-mediated post-transcriptional regulation is uncovered as an additional level of CtrA fine-tuning. Computational predictions as well as transcriptome and proteome studies consistently suggested targeting of ctrA and the putative cold shock chaperone cspA5 mRNAs by the trans-encoded sRNA (trans-sRNA) GspR (formerly SmelC775) in several Sinorhizobium species. GspR strongly accumulated in the stationary growth phase, especially in minimal medium (MM) cultures. Lack of the gspR locus confers a fitness disadvantage in competition with the wild type, while its overproduction hampers cell growth, suggesting that this riboregulator interferes with cell cycle progression. An eGFP-based reporter in vivo assay, involving wild-type and mutant sRNA and mRNA pairs, experimentally confirmed GspR-dependent post-transcriptional down-regulation of ctrA and cspA5 expression, which most likely occurs through base-pairing to the respective mRNA. The energetically favored secondary structure of GspR is predicted to comprise three stem-loop domains, with stem-loop 1 and stem-loop 3 targeting ctrA and cspA5 mRNA, respectively. Moreover, this work reports evidence for post-transcriptional control of ctrA by CspA5. Thus, this regulation and GspR-mediated post-transcriptional repression of ctrA and cspA5 expression constitute a coherent feed-forward loop, which may enhance the negative effect of GspR on CtrA levels. This novel regulatory circuit involving the riboregulator GspR, CtrA, and a cold shock chaperone may contribute to fine-tuning of ctrA expression. PMID:29740411

An sRNA and Cold Shock Protein Homolog-Based Feedforward Loop Post-transcriptionally Controls Cell Cycle Master Regulator CtrA.

PubMed

Robledo, Marta; Schlüter, Jan-Philip; Loehr, Lars O; Linne, Uwe; Albaum, Stefan P; Jiménez-Zurdo, José I; Becker, Anke

2018-01-01

Adjustment of cell cycle progression is crucial for bacterial survival and adaptation under adverse conditions. However, the understanding of modulation of cell cycle control in response to environmental changes is rather incomplete. In α-proteobacteria, the broadly conserved cell cycle master regulator CtrA underlies multiple levels of control, including coupling of cell cycle and cell differentiation. CtrA levels are known to be tightly controlled through diverse transcriptional and post-translational mechanisms. Here, small RNA (sRNA)-mediated post-transcriptional regulation is uncovered as an additional level of CtrA fine-tuning. Computational predictions as well as transcriptome and proteome studies consistently suggested targeting of ctrA and the putative cold shock chaperone cspA5 mRNAs by the trans- encoded sRNA ( trans- sRNA) GspR (formerly SmelC775) in several Sinorhizobium species. GspR strongly accumulated in the stationary growth phase, especially in minimal medium (MM) cultures. Lack of the gspR locus confers a fitness disadvantage in competition with the wild type, while its overproduction hampers cell growth, suggesting that this riboregulator interferes with cell cycle progression. An eGFP-based reporter in vivo assay, involving wild-type and mutant sRNA and mRNA pairs, experimentally confirmed GspR-dependent post-transcriptional down-regulation of ctrA and cspA5 expression, which most likely occurs through base-pairing to the respective mRNA. The energetically favored secondary structure of GspR is predicted to comprise three stem-loop domains, with stem-loop 1 and stem-loop 3 targeting ctrA and cspA5 mRNA, respectively. Moreover, this work reports evidence for post-transcriptional control of ctrA by CspA5. Thus, this regulation and GspR-mediated post-transcriptional repression of ctrA and cspA5 expression constitute a coherent feed-forward loop, which may enhance the negative effect of GspR on CtrA levels. This novel regulatory circuit involving the riboregulator GspR, CtrA, and a cold shock chaperone may contribute to fine-tuning of ctrA expression.

N-Glycosylation of Asparagine 130 in the Extracellular Domain of the Human Calcitonin Receptor Significantly Increases Peptide Hormone Affinity.

PubMed

Lee, Sang-Min; Booe, Jason M; Gingell, Joseph J; Sjoelund, Virginie; Hay, Debbie L; Pioszak, Augen A

2017-07-05

The calcitonin receptor (CTR) is a class B G protein-coupled receptor that is activated by the peptide hormones calcitonin and amylin. Calcitonin regulates bone remodeling through CTR, whereas amylin regulates blood glucose and food intake by activating CTR in complex with receptor activity-modifying proteins (RAMPs). These receptors are targeted clinically for the treatment of osteoporosis and diabetes. Here, we define the role of CTR N-glycosylation in hormone binding using purified calcitonin and amylin receptor extracellular domain (ECD) glycoforms and fluorescence polarization/anisotropy and isothermal titration calorimetry peptide-binding assays. N-Glycan-free CTR ECD produced in Escherichia coli exhibited ∼10-fold lower peptide affinity than CTR ECD produced in HEK293T cells, which yield complex N-glycans, or in HEK293S GnTI - cells, which yield core N-glycans (Man 5 GlcNAc 2 ). PNGase F-catalyzed removal of N-glycans at N73, N125, and N130 in the CTR ECD decreased peptide affinity ∼10-fold, whereas Endo H-catalyzed trimming of the N-glycans to single GlcNAc residues had no effect on peptide binding. Similar results were observed for an amylin receptor RAMP2-CTR ECD complex. Characterization of peptide-binding affinities of purified N → Q CTR ECD glycan site mutants combined with PNGase F and Endo H treatment strategies and mass spectrometry to define the glycan species indicated that a single GlcNAc residue at CTR N130 was responsible for the peptide affinity enhancement. Molecular modeling suggested that this GlcNAc functions through an allosteric mechanism rather than by directly contacting the peptide. These results reveal an important role for N-linked glycosylation in the peptide hormone binding of a clinically relevant class B GPCR.

Ethylene Regulates Levels of Ethylene Receptor/CTR1 Signaling Complexes in Arabidopsis thaliana

DOE PAGES

Shakeel, Samina N.; Gao, Zhiyong; Amir, Madiha; ...

2015-03-26

The plant hormone ethylene is perceived by a five-member family of receptors in Arabidopsis thaliana. The receptors function in conjunction with the Raf-like kinase CTR1 to negatively regulate ethylene signal transduction. CTR1 interacts with multiple members of the receptor family based on co-purification analysis, interacting more strongly with receptors containing a receiver domain. Levels of membrane-associated CTR1 vary in response to ethylene, doing so in a post-transcriptional manner that correlates with ethylene-mediated changes in levels of the ethylene receptors ERS1, ERS2, EIN4, and ETR2. Interactions between CTR1 and the receptor ETR1 protect ETR1 from ethylene-induced turnover. Kinetic and dose-response analysesmore » support a model in which two opposing factors control levels of the ethylene receptor/CTR1 complexes. Ethylene stimulates the production of new complexes largely through transcriptional induction of the receptors. However, ethylene also induces turnover of receptors, such that levels of ethylene receptor/CTR1 complexes decrease at higher ethylene concentrations. Lastly, we discuss implications of this model for ethylene signaling.« less

Ethylene Regulates Levels of Ethylene Receptor/CTR1 Signaling Complexes in Arabidopsis thaliana*

PubMed Central

Shakeel, Samina N.; Gao, Zhiyong; Amir, Madiha; Chen, Yi-Feng; Rai, Muneeza Iqbal; Haq, Noor Ul; Schaller, G. Eric

2015-01-01

The plant hormone ethylene is perceived by a five-member family of receptors in Arabidopsis thaliana. The receptors function in conjunction with the Raf-like kinase CTR1 to negatively regulate ethylene signal transduction. CTR1 interacts with multiple members of the receptor family based on co-purification analysis, interacting more strongly with receptors containing a receiver domain. Levels of membrane-associated CTR1 vary in response to ethylene, doing so in a post-transcriptional manner that correlates with ethylene-mediated changes in levels of the ethylene receptors ERS1, ERS2, EIN4, and ETR2. Interactions between CTR1 and the receptor ETR1 protect ETR1 from ethylene-induced turnover. Kinetic and dose-response analyses support a model in which two opposing factors control levels of the ethylene receptor/CTR1 complexes. Ethylene stimulates the production of new complexes largely through transcriptional induction of the receptors. However, ethylene also induces turnover of receptors, such that levels of ethylene receptor/CTR1 complexes decrease at higher ethylene concentrations. Implications of this model for ethylene signaling are discussed. PMID:25814663

Primary site and regional lymph node involvement are independent prognostic factors for early-stage extranodal nasal-type natural killer/T cell lymphoma.

PubMed

Niu, Shao-Qing; Yang, Yong; Li, Yi-Yang; Wen, Ge; Wang, Liang; Li, Zhi-Ming; Wang, Han-Yu; Zhang, Lu-Lu; Xia, Yun-Fei; Zhang, Yu-Jing

2016-04-04

Nasal-type extranodal natural killer/T-cell lymphoma (ENKTCL) originates primarily in the nasal cavity or extra-nasal sites within the upper aerodigestive tract. However, it is unclear whether the primary site can serve as an independent prognostic factor or whether the varying clinical outcomes observed with different primary sites can be attributed merely to their propensities of regional lymph node involvement. The aim of this study was to investigate the prognostic implications of the primary site and regional lymph node involvement in patients with early-stage nasal-type ENKTCL. To develop a nomogram, we reviewed the clinical data of 215 consecutively diagnosed patients with early-stage nasal-type ENKTCL who were treated in Sun Yat-sen University Cancer Center with chemotherapy and radiotherapy between 2000 and 2011. The predictive accuracy and discriminative ability of the nomogram were determined using a concordance index (C-index) and calibration curve. The 5-year overall survival (OS) and progression-free survival (PFS) rates of patients with nasal ENKTCL were higher than those of patients with extra-nasal ENKTCL (OS: 68.2% vs. 46.0%, P = 0.030; PFS: 53.4% vs. 26.6%, P = 0.010). The 5-year OS and PFS rates of patients with Ann Arbor stage IE ENKTCL were higher than those of patients with Ann Arbor stage IIE ENKTCL (OS: 66.3% vs. 59.2%, P = 0.003; PFS: 51.4% vs. 40.3%, P = 0.009). Multivariate analysis showed that age >60 years, ECOG performance status score ≥2, elevated lactate dehydrogenase (LDH) level, extra-nasal primary site, and regional lymph node involvement were significantly associated with lower 5-year OS rate; age >60 years, elevated LDH level, extra-nasal primary site, and regional lymph node involvement were significantly associated with lower 5-year PFS rate. The nomogram included the primary site and regional lymph node involvement based on multivariate analysis. The calibration curve showed good agreement between the predicted and actual 5-year OS and PFS rates, and the C-indexes of the nomogram for the OS and PFS rates were 0.697 and 0.634, respectively. The primary site and regional lymph node involvement are independent prognostic factors for early-stage ENKTCL treated with chemotherapy followed by definitive radiotherapy.

Thiamine Deficiency Complex Workshop final report: November 6-7, 2008, Ann Arbor, MI

USGS Publications Warehouse

Honeyfield, Dale C.; Tillitt, Donald E.; Riley, Stephen C.

2008-01-01

Fry mortality which was first observed in the late 1960s in Great Lakes salmonines and in Baltic Sea salmon in 1974 has now been linked to thiamine deficiency (historically referred to as Early Mortality Syndrome, or EMS and M74, respectively). Over the past 14 years significant strides have been made in our understanding of this perplexing problem. It is now known that thiamine deficiency causes embryonic mortality in these salmonids. Both overt mortality and secondary effects of thiamine deficiency are observed in juvenile and adult animals. Collectively the morbidity and mortality (fry and adult mortality, secondary metabolic and behavior affects in juveniles and adult fish) are referred to as Thiamine Deficiency Complex (TDC). A workshop was held in Ann Arbor, MI on 6-7 November 2008 that brought together 38 federal, state, provincial, tribal and university scientists to share information, present data and discuss the latest observations on thiamine status of aquatic animals with thiamine deficiency and the causative agent, thiaminase. Twenty presentations (13 oral and 7 posters) detailed current knowledge. In Lake Huron, low alewife Alosa pseudoharengus abundance has persisted and egg thiamine concentrations in salmonines continue to increase, along with evidence of natural reproduction in lake trout Salvelinus namaycush. Lake Michigan Chinook salmon Oncorhynchus tshawytscha appear to have a lower thiamine requirement than other salmonids in the lake. Lake Ontario American eel Anguilla rostrata foraging on alewife have approximately one third the muscle thiamine compared to eels not feeding on alewife, suggesting that eels may be suffering from thiamine deficiency. Secondary effects of low thiamine exist in Great Lakes salmonines and should not be ignored. Thiaminase activity in dreissenid mussels is extremely high but a connection to TDC has not been made. Thiaminase in net plankton was found more consistently in lakes Michigan and Ontario than other lakes evaluated. The biological role of thiaminase I, associated with thiamine deficiency, remains to be determined whereas thiaminase II has been reported to be part of a salvage pathway leading to thiamine synthesis. The use of gene array technology and 3-dimensional histology is adding new understanding to the affects of thiamine deficiency. Research is needed to determine the thiamine status of species feeding on dreissenids, the environmental sources of thiaminase and the biological role of thiaminase I.

Detection of increased 64Cu uptake by human copper transporter 1 gene overexpression using PET with 64CuCl2 in human breast cancer xenograft model.

PubMed

Kim, Kwang Il; Jang, Su Jin; Park, Ju Hui; Lee, Yong Jin; Lee, Tae Sup; Woo, Kwang Sun; Park, Hyun; Choe, Jae Gol; An, Gwang Il; Kang, Joo Hyun

2014-10-01

Copper is an essential cofactor for a variety of biochemical processes including oxidative phosphorylation, cellular antioxidant activity, and elimination of free radicals. The copper transporter 1 is known to be involved in cellular uptake of copper ions. In this study, we evaluated the utility of human copper transporter 1 (hCTR1) gene as a new reporter gene for (64)Cu PET imaging. Human breast cancer cells (MDA-MB-231) were infected with a lentiviral vector constitutively expressing the hCTR1 gene under super cytomegalovirus promoter, and positive clones (MDA-MB-231-hCTR1) were selected. The expression of hCTR1 gene in MDA-MB-231-hCTR1 cells was measured by reverse transcription polymerase chain reaction, Western blot, and (64)Cu uptake assay. To evaluate the cytotoxic effects induced by hCTR1 expression, the dose-dependent cell survival rate after treatment with cisplatin (Cis-diaminedichloroplatinum (II) [CDDP]) was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and trypan blue dye exclusion. Small-animal PET images were acquired in tumor-bearing mice from 2 to 48 h after an intravenous injection of (64)Cu. The hCTR1 gene expression in MDA-MB-231-hCTR1 cells was confirmed at the RNA and protein expression and the cellular (64)Cu uptake level. MTT assay and trypan blue dye exclusion showed that the cell viability of MDA-MB-231-hCTR1 cells decreased more rapidly than that of MDA-MB-231 cells after treatment with CDDP for 96 or 72 h, respectively. Small-animal PET imaging revealed a higher accumulation of (64)Cu in MDA-MB-231-hCTR1 tumors than in MDA-MB-231 tumors. With respect to the biodistribution data, the percentage injected dose per gram of (64)Cu in the MDA-MB-231 tumors and MDA-MB-231-hCTR1 tumors at 48 h after (64)Cu injection was 2.581 ± 0.254 and 5.373 ± 1.098, respectively. An increase in (64)Cu uptake induced by the expression of hCTR1 gene was demonstrated in vivo and in vitro, suggesting the potential use of hCTR1 gene as a new imaging reporter gene for PET with (64)CuCl2. © 2014 by the Society of Nuclear Medicine and Molecular Imaging, Inc.

Chondrogenesis of Human Adipose-Derived Stem Cells by In Vivo Co-graft with Auricular Chondrocytes from Microtia.

PubMed

Cai, Zhen; Pan, Bo; Jiang, Haiyue; Zhang, Lixia

2015-06-01

To evaluate the efficiency of chondrogenesis of human adipose-derived stem cells (ADSCs) induced by auricular chondrocytes from microtia via subcutaneous co-graft in nude mice. Human ADSCs and auricular chondrocytes were mixed at the ratio of 7:3 and suspended in 0.2 ml of Pluronic F-127 (5.0 × 10(7) cells/ml), and injected into Balb/c nude mice as the experimental group (Exp group). The same quantity of auricular chondrocytes (Ctr.1 group) or ADSCs (Ctr.2 group) in 0.2 ml of Pluronic F-127 was set as positive and negative control groups. The mixture of auricular chondrocytes (1.5 × 10(7) cells/ml) in 0.2 ml of Pluronic F-127 was set as the low concentration of chondrocyte control group (Ctr.3). At 8 weeks after grafting, the newly generated tissue pellets were isolated for morphological examination, haematoxylin and eosin staining, toluidine blue staining and safranin O staining of glycosaminoglycan (GAG), Masson's trichrome staining and immunohistochemical staining of type II collagen, and Verhoeff-iron-hematoxylin staining of elastic fibers. GAG content was determined by Alcian blue colorimetric method, and mRNA expression of type II collagen and aggrecan were examined by real-time PCR. Cartilage-like tissue with a white translucent appearance and good elasticity was generated in the Exp and Ctr.1 groups. The tissue pellets in the Ctr.2 and Ctr.3 groups were much smaller than those in the Ctr.1 group. The mature cartilage lacunas could be observed in the Exp and Ctr.1 groups, while were rarely seen in the Ctr.3 group and not observed in the Ctr.2 group. The expression of cartilage-specific extracellular matrix such as type II collagen, GAG content, aggrecan, and elastic fibers in the Exp group was similar to that in the Ctr.1 group, whereas the expression of these extracellular matrix substances was significantly lower in the Ctr.2 and Ctr.3 groups (both P < 0.01). Auricular chondrocytes from microtia can efficiently promote the chondrogenic differentiation and chondrogenesis of ADSCs by co-grafting in vivo. This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors http://www.springer.com/00266 .

Surgical Approach and Anesthetic Modality for Carpal Tunnel Release: A Nationwide Database Study With Health Care Cost Implications.

PubMed

Foster, Brock D; Sivasundaram, Lakshmanan; Heckmann, Nathanael; Cohen, Jeremiah R; Pannell, William C; Wang, Jeffrey C; Ghiassi, Alidad

2017-03-01

Background: Carpal tunnel release (CTR) is commonly performed for carpal tunnel syndrome once conservative treatment has failed. Operative technique and anesthetic modality vary by surgeon preference and patient factors. However, CTR practices and anesthetic trends have, to date, not been described on a nationwide scale in the United States. Methods: The PearlDiver Patient Records Database was used to search Current Procedural Terminology codes for elective CTR from 2007 to 2011. Anesthetic modality (eg, general and regional anesthesia vs local anesthesia) and surgical approach (eg, endoscopic vs open) were recorded for this patient population. Cost analysis, patient demographics, regional variation, and annual changes in CTR surgery were evaluated. Results: We identified 86 687 patients who underwent carpal tunnel surgery during this 5-year time period. In this patient sample, 80.5% of CTR procedures were performed using general or regional anesthesia, compared with 19.5% of procedures performed using local anesthesia; 83.9% of all CTR were performed in an open fashion, and 16.1% were performed using an endoscopic technique. Endoscopic surgery was on average $794 more expensive than open surgery, and general or regional anesthesia was $654 more costly than local anesthesia. Conclusions: In the United States, open CTR under local anesthesia is the most cost-effective way to perform a CTR. However, only a small fraction of elective CTR procedures are performed with this technique, representing a potential area for significant health care cost savings. In addition, regional and age variations exist in procedure and anesthetic type utilized.

[The modification of electrophysiology affected by ectopic synapse in ON-retinal bipolar cells of RCS rats].

PubMed

Zhang, Chen-xing; Yin, Zheng-qin; Weng, Chuan-huang; Zeng, Yu-xiao

2011-03-01

To study the influence of the ectopic synapse for electrophysiological characteristics modification in ON retinal bipolar cells (ON-RBCs) of RCS rat. Immunofluorescence of the retinal frozen section was taken in P60 d, P90 d of RCS rat (RCS) and control rat (CTR) with the anti-mGluR6 and anti-Synaptophysin, Lucifer Yellow staining solo ON-RBCs was taken in all the group. The whole cell recording was performed in the retinal slice of P60 d, P90 d in RCS and CTR. The modification of the passive membrane properties and the outward currents properties in RCS-ON-RBCs, CTR-ON-RBCs and CTR-OFF-RBCs were observed. RCS-ON-RBCs stretched out the ectopic neurite in different direction and the activity of synapse could be detected around the ectopic neurite. From Pn60d, passive membrane properties of RCS-ON-RBCs kept immature, The RMP in RCS-ON-RBCs and CTR-ON-RBCs were (-61.8 ± 3.07), (-50.44 ± 1.36) mV and (-63.1 ± 2.59), (-48.37 ± 3.69) mV when P60 d and P90 d, there ware significantly higher than CTR group (t = 2.191, 2.435, 5.817, 6.912;P < 0.05). The IR in RCS-ON-RBCs and CTR-ON-RBCs were (323.3 ± 42.6), (337.6 ± 71.3) MΩ and (321.2 ± 58.6), (340.3 ± 62.8) MΩ when P60 d and P90 d, there ware significantly higher than CTR group (t = 3.561, 1.987, 5.211, 4.034; P < 0.05). Outward currents were recorded when giving hyper- and depolarized voltage steps. In retinal degeneration, the amplitude of outward currents in RCS-ON-RBCs is significantly different with CTR-ON-RBCs (t = 5.561, 6.341; P < 0.05) or CTR-OFF-RBCs (t = 5.357, 6.997; P < 0.05). The ectopic neurite from RCS-ON-RBCs has the possibility for translating the signal. In retinitis pigmentosa, the modification of electrophysiology characteristics in RCS-ON-RBCs was significantly different with CTR-ON-RBCs and CTR-OFF-RBCs. Influence with the ectopic neurite is the possible cause.

Ursodeoxycholic acid improves gastrointestinal motility defects in gallstone patients

PubMed Central

Colecchia, A; Mazzella, G; Sandri, L; Azzaroli, F; Magliuolo, M; Simoni, P; Bacchi-Reggiani, ML; Roda, E; Festi, D

2006-01-01

AIM: To simultaneously evaluate the presence of defects in gallbladder and gastric emptying, as well as in intestinal transit in gallstone patients (GS) and the effect of chronic ursodeoxycholic acid (UDCA) administration on these parameters and on serum bile acids and clinical outcome in GS and controls (CTR). METHODS: After a standard liquid test meal, gallbla-dder and gastric emptying (by ultrasound), oroileal transit time (OITT) (by an immunoenzymatic technique) and serum bile acids (by HPLC) were evaluated before and after 3 mo of UDCA (12 mg/kg bw/d) or placebo administration in 10 symptomatic GS and 10 matched healthy CTR. RESULTS: OITT was longer in GS than in CTR (P < 0.0001); UDCA significantly reduced OITT in GS (P < 0.0001), but not in CTR. GS had longer gastric half-emptying time (t1/2) than CTR (P < 0.0044) at baseline; after UDCA, t1/2 significantly decreased (P < 0.006) in GS but not in CTR. Placebo administration had no effect on gastric emptying and intestinal transit in both GS and CTR. CONCLUSION: The gallstone patient has simultaneous multiple impairments of gallbladder and gastric emptying, as well as of intestinal transit. UDCA administration restores these defects in GS, without any effect in CTR. These results confirm the pathogenetic role of gastrointestinal motility in gallstone disease and suggest an additional mechanism of action for UDCA in reducing bile cholesterol supersaturation. PMID:16981264

Sinorhizobium meliloti CtrA Stability Is Regulated in a CbrA-Dependent Manner That Is Influenced by CpdR1

PubMed Central

Schallies, Karla B.; Sadowski, Craig; Meng, Julia; Chien, Peter

2015-01-01

ABSTRACT CbrA is a DivJ/PleC-like histidine kinase of DivK that is required for cell cycle progression and symbiosis in the alphaproteobacterium Sinorhizobium meliloti. Loss of cbrA results in increased levels of CtrA as well as its phosphorylation. While many of the known Caulobacter crescentus regulators of CtrA phosphorylation and proteolysis are phylogenetically conserved within S. meliloti, the latter lacks the PopA regulator that is required for CtrA degradation in C. crescentus. In order to investigate whether CtrA proteolysis occurs in S. meliloti, CtrA stability was assessed. During exponential growth, CtrA is unstable and therefore likely to be degraded in a cell cycle-regulated manner. Loss of cbrA significantly increases CtrA stability, but this phenotype is restored to that of the wild type by constitutive ectopic expression of a CpdR1 variant that cannot be phosphorylated (CpdR1D53A). Addition of CpdR1D53A fully suppresses cbrA mutant cell cycle defects, consistent with regulation of CtrA stability playing a key role in mediating proper cell cycle progression in S. meliloti. Importantly, the cbrA mutant symbiosis defect is also suppressed in the presence of CpdR1D53A. Thus, regulation of CtrA stability by CbrA and CpdR1 is associated with free-living cell cycle outcomes and symbiosis. IMPORTANCE The cell cycle is a fundamental process required for bacterial growth, reproduction, and developmental differentiation. Our objective is to understand how a two-component signal transduction network directs cell cycle events during free-living growth and host colonization. The Sinorhizobium meliloti nitrogen-fixing symbiosis with plants is associated with novel cell cycle events. This study identifies a link between the regulated stability of an essential response regulator, free-living cell cycle progression, and symbiosis. PMID:25897034

The Cu(II) affinity of the N-terminus of human copper transporter CTR1: Comparison of human and mouse sequences.

PubMed

Bossak, Karolina; Drew, Simon C; Stefaniak, Ewelina; Płonka, Dawid; Bonna, Arkadiusz; Bal, Wojciech

2018-05-01

Copper Transporter 1 (CTR1) is a homotrimeric membrane protein providing the main route of copper transport into eukaryotic cells from the extracellular milieu. Its N-terminal extracellular domain, rich in His and Met residues, is considered responsible for directing copper into the transmembrane channel. Most of vertebrate CTR1 proteins contain the His residue in position three from N-terminus, creating a well-known Amino Terminal Cu(II)- and Ni(II)-Binding (ATCUN) site. CTR1 from humans, primates and many other species contains the Met-Asp-His (MDH) sequence, while some rodents including mouse have the Met-Asn-His (MNH) N-terminal sequence. CTR1 is thought to collect Cu(II) ions from blood copper transport proteins, including albumin, but previous reports indicated that the affinity of N-terminal peptide/domain of CTR1 is significantly lower than that of albumin, casting serious doubt on this aspect of CTR1 function. Using potentiometry and spectroscopic techniques we demonstrated that MDH-amide, a tripeptide model of human CTR1 N-terminus, binds Cu(II) with K of 1.3 × 10 13  M -1 at pH 7.4, ~13 times stronger than Human Serum Albumin (HSA), and MNH-amide is even stronger, K of 3.2 × 10 14  M -1 at pH 7.4. These results indicate that the N-terminus of CTR1 may serve as intermediate binding site during Cu(II) transfer from blood copper carriers to the transporter. MDH-amide, but not MNH-amide also forms a low abundance complex with non-ATCUN coordination involving the Met amine, His imidazole and Asp carboxylate. This species might assist Cu(II) relay down the peptide chain or its reduction to Cu(I), both steps necessary for the CTR1 function. Copyright © 2018 Elsevier Inc. All rights reserved.

Calcitonin and Amylin Receptor Peptide Interaction Mechanisms

PubMed Central

Lee, Sang-Min; Hay, Debbie L.; Pioszak, Augen A.

2016-01-01

Receptor activity-modifying proteins (RAMP1–3) determine the selectivity of the class B G protein-coupled calcitonin receptor (CTR) and the CTR-like receptor (CLR) for calcitonin (CT), amylin (Amy), calcitonin gene-related peptide (CGRP), and adrenomedullin (AM) peptides. RAMP1/2 alter CLR selectivity for CGRP/AM in part by RAMP1 Trp-84 or RAMP2 Glu-101 contacting the distinct CGRP/AM C-terminal residues. It is unclear whether RAMPs use a similar mechanism to modulate CTR affinity for CT and Amy, analogs of which are therapeutics for bone disorders and diabetes, respectively. Here, we reproduced the peptide selectivity of intact CTR, AMY1 (CTR·RAMP1), and AMY2 (CTR·RAMP2) receptors using purified CTR extracellular domain (ECD) and tethered RAMP1- and RAMP2-CTR ECD fusion proteins and antagonist peptides. All three proteins bound salmon calcitonin (sCT). Tethering RAMPs to CTR enhanced binding of rAmy, CGRP, and the AMY antagonist AC413. Peptide alanine-scanning mutagenesis and modeling of receptor-bound sCT and AC413 supported a shared non-helical CGRP-like conformation for their TN(T/V)G motif prior to the C terminus. After this motif, the peptides diverged; the sCT C-terminal Pro was crucial for receptor binding, whereas the AC413/rAmy C-terminal Tyr had little or no influence on binding. Accordingly, mutant RAMP1 W84A- and RAMP2 E101A-CTR ECD retained AC413/rAmy binding. ECD binding and cell-based signaling assays with antagonist sCT/AC413/rAmy variants with C-terminal residue swaps indicated that the C-terminal sCT/rAmy residue identity affects affinity more than selectivity. rAmy(8–37) Y37P exhibited enhanced antagonism of AMY1 while retaining selectivity. These results reveal unexpected differences in how RAMPs determine CTR and CLR peptide selectivity and support the hypothesis that RAMPs allosterically modulate CTR peptide affinity. PMID:26895962

Influence of depth of interaction upon the performance of scintillator detectors.

PubMed

Brown, Mark S; Gundacker, Stefan; Taylor, Alaric; Tummeltshammer, Clemens; Auffray, Etiennette; Lecoq, Paul; Papakonstantinou, Ioannis

2014-01-01

The uncertainty in time of particle detection within a scintillator detector, characterised by the coincidence time resolution (CTR), is explored with respect to the interaction position within the scintillator crystal itself. Electronic collimation between two scintillator detectors is utilised to determine the CTR with depth of interaction (DOI) for different materials, geometries and wrappings. Significantly, no relationship between the CTR and DOI is observed within experimental error. Confinement of the interaction position is seen to degrade the CTR in long scintillator crystals by 10%.

Cardiothoracic ratio within the “normal” range independently predicts mortality in patients undergoing coronary angiography

PubMed Central

Zaman, M Justin S; Sanders, Julie; Crook, Angela M; Feder, Gene; Shipley, Martin; Timmis, Adam; Hemingway, Harry

2007-01-01

Objective To determine whether cardiothoracic ratio (CTR), within the range conventionally considered normal, predicted prognosis in patients undergoing coronary angiography. Design Cohort study with a median of 7‐years follow‐up. Setting Consecutive patients undergoing coronary angiography at Barts and The London National Health Service (NHS) Trust. Subjects 1005 patients with CTRs measured by chest radiography, and who subsequently underwent coronary angiography. Of these patients, 7.3% had a CTR ⩾0.5 and were excluded from the analyses. Outcomes All‐cause mortality and coronary event (non‐fatal myocardial infarction or coronary death). Adjustments were made for age, left ventricular dysfunction, ACE inhibitor treatment, body mass index, number of diseased coronary vessels and past coronary artery bypass graft. Results The risk of death was increased among patients with a CTR in the upper part of the normal range. In total, 94 (18.9%) of those with a CTR below the median of 0.42 died compared with 120 (27.8%) of those with a CTR between 0.42 and 0.49 (log rank test p<0.001). After adjusting for potential confounders, this increased risk remained (adjusted HR 1.45, 95% CI 1.03 to 2.05). CTR, at values below 0.5, was linearly related to the risk of coronary event (test for trend p = 0.024). Conclusion : In patients undergoing coronary angiography, CTR between 0.42 and 0.49 was associated with higher mortality than in patients with smaller hearts. There was evidence of a continuous increase in risk with higher CTR. These findings, along with those in healthy populations, question the conventional textbook cut‐off point of ⩾0.5 being an abnormal CTR. PMID:17164481

An Assessment of Civil Tiltrotor Concept of Operations in the Next Generation Air Transportation System

NASA Technical Reports Server (NTRS)

Chung, William W.; Salvano, Dan; Rinehart, David; Young, Ray; Cheng, Victor; Lindsey, James

2012-01-01

Based on a previous Civil Tiltrotor (CTR) National Airspace System (NAS) performance analysis study, CTR operations were evaluated over selected routes and terminal airspace configurations assuming noninterference operations (NIO) and runway-independent operations (RIO). This assessment aims to further identify issues associated with these concepts of operations (ConOps), and their dependency on the airspace configuration and interaction with conventional fixed-wing traffic. Safety analysis following a traditional Safety Management System (SMS) methodology was applied to CTR-unique departure and arrival failures in the selected airspace to identify any operational and certification issues. Additional CTR operational cases were then developed to get a broader understanding of issues and gaps that will need to be addressed in future CTR operational studies. Finally, needed enhancements to National Airspace System performance analysis tools were reviewed, and recommendations were made on improvements in these tools that are likely to be required to support future progress toward CTR fleet operations in the Next Generation Air Transportation System (NextGen).

The protease ClpXP and the PAS-domain protein DivL regulate CtrA and gene transfer agent production in Rhodobacter capsulatus.

PubMed

Westbye, A B; Kater, L; Wiesmann, C; Ding, H; Yip, C K; Beatty, J T

2018-04-06

Several members of the Rhodobacterales ( Alphaproteobacteria ) produce a conserved horizontal gene transfer vector, called gene transfer agent (GTA), that appears to have evolved from a bacteriophage. The model system to study GTA biology is the R. capsulatus GTA (RcGTA), a small, tailed bacteriophage-like particle produced by a subset of the cells in a culture. The response regulator CtrA is conserved in the Alphaproteobacteria and is an essential regulator of RcGTA production: it controls production and maturation of the RcGTA particle and RcGTA release from cells. CtrA also controls the natural transformation-like system required for cells to receive RcGTA-donated DNA. Here, we report that a dysregulation of the CckA-ChpT-CtrA phosphorelay - either by loss of the PAS-domain protein DivL or by substituting the autophosphorylation residue of the hybrid histidine kinase CckA - decreased CtrA phosphorylation and greatly increased RcGTA protein production in R. capsulatus We show that a loss of the ClpXP protease or the three C-terminal residues of CtrA resulted in increased CtrA levels in R. capsulatus, and identify ClpX(P) as essential for maturation of RcGTA particles. Furthermore, we show that CtrA phosphorylation is important for head spike production. Our results provide novel insight into the regulation of CtrA and GTAs in the Rhodobacterales Importance Members of the Rhodobacterales are abundant in ocean and fresh water environments. The conserved GTA produced by many Rhodobacterales may have an important role in horizontal gene transfer (HGT) in aquatic environments and provide a significant contribution to their adaptation. GTA production is controlled by bacterial regulatory systems, including the conserved CckA-ChpT-CtrA phosphorelay, however several questions about GTA regulation remains. Our identification that a 'short' DivL homologue and ClpXP regulate CtrA in R. capsulatus extends the model of CtrA regulation from Caulobacter crescentus to a member of the Rhodobacterales. We found that the magnitude of RcGTA production greatly depended on DivL and CckA kinase activity, adding yet a layer of regulatory complexity to RcGTA. RcGTA is known to undergo CckA-dependent maturation, and we extend the understanding of this process by showing that the ClpX chaperone is required for formation of tailed, DNA-containing particles. Copyright © 2018 American Society for Microbiology.

Influence of Depth of Interaction upon the Performance of Scintillator Detectors

PubMed Central

Brown, Mark S.; Gundacker, Stefan; Taylor, Alaric; Tummeltshammer, Clemens; Auffray, Etiennette; Lecoq, Paul; Papakonstantinou, Ioannis

2014-01-01

The uncertainty in time of particle detection within a scintillator detector, characterised by the coinci- dence time resolution (CTR), is explored with respect to the interaction position within the scintillator crystal itself. Electronic collimation between two scintillator detectors is utilised to determine the CTR with depth of interaction (DOI) for different materials, geometries and wrappings. Significantly, no rela- tionship between the CTR and DOI is observed within experimental error. Confinement of the interaction position is seen to degrade the CTR in long scintillator crystals by 10%. PMID:24875832

Functional relevance of G-protein-coupled-receptor-associated proteins, exemplified by receptor-activity-modifying proteins (RAMPs).

PubMed

Fischer, J A; Muff, R; Born, W

2002-08-01

The calcitonin (CT) receptor (CTR) and the CTR-like receptor (CRLR) are close relatives within the type II family of G-protein-coupled receptors, demonstrating sequence identity of 50%. Unlike the interaction between CT and CTR, receptors for the related hormones and neuropeptides amylin, CT-gene-related peptide (CGRP) and adrenomedullin (AM) require one of three accessory receptor-activity-modifying proteins (RAMPs) for ligand recognition. An amylin/CGRP receptor is revealed when CTR is co-expressed with RAMP1. When complexed with RAMP3, CTR interacts with amylin alone. CRLR, initially classed as an orphan receptor, is a CGRP receptor when co-expressed with RAMP1. The same receptor is specific for AM in the presence of RAMP2. Together with human RAMP3, CRLR defines an AM receptor, and with mouse RAMP3 it is a low-affinity CGRP/AM receptor. CTR-RAMP1, antagonized preferentially by salmon CT-(8-32) and not by CGRP-(8-37), and CRLR-RAMP1, antagonized by CGRP-(8-37), are two CGRP receptor isotypes. Thus amylin and CGRP interact specifically with heterodimeric complexes between CTR and RAMP1 or RAMP3, and CGRP and AM interact with complexes between CRLR and RAMP1, RAMP2 or RAMP3.

Calcitonin and Amylin Receptor Peptide Interaction Mechanisms: INSIGHTS INTO PEPTIDE-BINDING MODES AND ALLOSTERIC MODULATION OF THE CALCITONIN RECEPTOR BY RECEPTOR ACTIVITY-MODIFYING PROTEINS.

PubMed

Lee, Sang-Min; Hay, Debbie L; Pioszak, Augen A

2016-04-15

Receptor activity-modifying proteins (RAMP1-3) determine the selectivity of the class B G protein-coupled calcitonin receptor (CTR) and the CTR-like receptor (CLR) for calcitonin (CT), amylin (Amy), calcitonin gene-related peptide (CGRP), and adrenomedullin (AM) peptides. RAMP1/2 alter CLR selectivity for CGRP/AM in part by RAMP1 Trp-84 or RAMP2 Glu-101 contacting the distinct CGRP/AM C-terminal residues. It is unclear whether RAMPs use a similar mechanism to modulate CTR affinity for CT and Amy, analogs of which are therapeutics for bone disorders and diabetes, respectively. Here, we reproduced the peptide selectivity of intact CTR, AMY1 (CTR·RAMP1), and AMY2 (CTR·RAMP2) receptors using purified CTR extracellular domain (ECD) and tethered RAMP1- and RAMP2-CTR ECD fusion proteins and antagonist peptides. All three proteins bound salmon calcitonin (sCT). Tethering RAMPs to CTR enhanced binding of rAmy, CGRP, and the AMY antagonist AC413. Peptide alanine-scanning mutagenesis and modeling of receptor-bound sCT and AC413 supported a shared non-helical CGRP-like conformation for their TN(T/V)G motif prior to the C terminus. After this motif, the peptides diverged; the sCT C-terminal Pro was crucial for receptor binding, whereas the AC413/rAmy C-terminal Tyr had little or no influence on binding. Accordingly, mutant RAMP1 W84A- and RAMP2 E101A-CTR ECD retained AC413/rAmy binding. ECD binding and cell-based signaling assays with antagonist sCT/AC413/rAmy variants with C-terminal residue swaps indicated that the C-terminal sCT/rAmy residue identity affects affinity more than selectivity. rAmy(8-37) Y37P exhibited enhanced antagonism of AMY1 while retaining selectivity. These results reveal unexpected differences in how RAMPs determine CTR and CLR peptide selectivity and support the hypothesis that RAMPs allosterically modulate CTR peptide affinity. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

Novel quinolone chalcones targeting colchicine-binding pocket kill multidrug-resistant cancer cells by inhibiting tubulin activity and MRP1 function.

PubMed

Lindamulage, I Kalhari; Vu, Hai-Yen; Karthikeyan, Chandrabose; Knockleby, James; Lee, Yi-Fang; Trivedi, Piyush; Lee, Hoyun

2017-08-31

Agents targeting colchicine-binding pocket usually show a minimal drug-resistance issue, albeit often associated with high toxicity. Chalcone-based compounds, which may bind to colchicine-binding site, are found in many edible fruits, suggesting that they can be effective drugs with less toxicity. Therefore, we synthesized and examined 24 quinolone chalcone compounds, from which we identified ((E)-3-(3-(2-Methoxyphenyl)-3-oxoprop-1-enyl) quinolin-2(1H)-one) (CTR-17) and ((E)-6-Methoxy-3-(3-(2-methoxyphenyl)-3-oxoprop-1-enyl) quinolin-2(1H)-one) (CTR-20) as promising leads. In particular, CTR-20 was effective against 65 different cancer cell lines originated from 12 different tissues, largely in a cancer cell-specific manner. We found that both CTR-17 and CTR-20 reversibly bind to the colchicine-binding pocket on β-tubulin. Interestingly however, both the CTRs were highly effective against multidrug-resistant cancer cells while colchicine, paclitaxel and vinblastine were not. Our study with CTR-20 showed that it overcomes multidrug-resistance through its ability to impede MRP1 function while maintaining strong inhibition against microtubule activity. Data from mice engrafted with the MDA-MB-231 triple-negative breast cancer cells showed that both CTR-17 and CTR-20 possess strong anticancer activity, alone or in combination with paclitaxel, without causing any notable side effects. Together, our data demonstrates that both the CTRs can be effective and safe drugs against many different cancers, especially against multidrug-resistant tumors.

Automatic estimation of heart boundaries and cardiothoracic ratio from chest x-ray images

NASA Astrophysics Data System (ADS)

Dallal, Ahmed H.; Agarwal, Chirag; Arbabshirani, Mohammad R.; Patel, Aalpen; Moore, Gregory

2017-03-01

Cardiothoracic ratio (CTR) is a widely used radiographic index to assess heart size on chest X-rays (CXRs). Recent studies have suggested that also two-dimensional CTR might contain clinical information about the heart function. However, manual measurement of such indices is both subjective and time consuming. This study proposes a fast algorithm to automatically estimate CTR indices based on CXRs. The algorithm has three main steps: 1) model based lung segmentation, 2) estimation of heart boundaries from lung contours, and 3) computation of cardiothoracic indices from the estimated boundaries. We extended a previously employed lung detection algorithm to automatically estimate heart boundaries without using ground truth heart markings. We used two datasets: a publicly available dataset with 247 images as well as clinical dataset with 167 studies from Geisinger Health System. The models of lung fields are learned from both datasets. The lung regions in a given test image are estimated by registering the learned models to patient CXRs. Then, heart region is estimated by applying Harris operator on segmented lung fields to detect the corner points corresponding to the heart boundaries. The algorithm calculates three indices, CTR1D, CTR2D, and cardiothoracic area ratio (CTAR). The method was tested on 103 clinical CXRs and average error rates of 7.9%, 25.5%, and 26.4% (for CTR1D, CTR2D, and CTAR respectively) were achieved. The proposed method outperforms previous CTR estimation methods without using any heart templates. This method can have important clinical implications as it can provide fast and accurate estimate of cardiothoracic indices.

Alternate Splicing of CD44 Messenger RNA in Prostate Cancer Growth

DTIC Science & Technology

2009-10-01

CT-cells have endog- enous CT stably knocked down to undetectable levels using anti-CT hammerhead ribozymes [25]. Salmon CT (BAChem, Torrance, CA) was...and cells called CTR-, derived from PC-3M cells after anti-CT receptor ribozyme knock- down of CTR[18]. CTR-cells have very low levels of CD44v

Prediction of clinical response to drugs in ovarian cancer using the chemotherapy resistance test (CTR-test).

PubMed

Kischkel, Frank Christian; Meyer, Carina; Eich, Julia; Nassir, Mani; Mentze, Monika; Braicu, Ioana; Kopp-Schneider, Annette; Sehouli, Jalid

2017-10-27

In order to validate if the test result of the Chemotherapy Resistance Test (CTR-Test) is able to predict the resistances or sensitivities of tumors in ovarian cancer patients to drugs, the CTR-Test result and the corresponding clinical response of individual patients were correlated retrospectively. Results were compared to previous recorded correlations. The CTR-Test was performed on tumor samples from 52 ovarian cancer patients for specific chemotherapeutic drugs. Patients were treated with monotherapies or drug combinations. Resistances were classified as extreme (ER), medium (MR) or slight (SR) resistance in the CTR-Test. Combination treatment resistances were transformed by a scoring system into these classifications. Accurate sensitivity prediction was accomplished in 79% of the cases and accurate prediction of resistance in 100% of the cases in the total data set. The data set of single agent treatment and drug combination treatment were analyzed individually. Single agent treatment lead to an accurate sensitivity in 44% of the cases and the drug combination to 95% accuracy. The detection of resistances was in both cases to 100% correct. ROC curve analysis indicates that the CTR-Test result correlates with the clinical response, at least for the combination chemotherapy. Those values are similar or better than the values from a publication from 1990. Chemotherapy resistance testing in vitro via the CTR-Test is able to accurately detect resistances in ovarian cancer patients. These numbers confirm and even exceed results published in 1990. Better sensitivity detection might be caused by a higher percentage of drug combinations tested in 2012 compared to 1990. Our study confirms the functionality of the CTR-Test to plan an efficient chemotherapeutic treatment for ovarian cancer patients.

Speckle tracking as a method to measure hemidiaphragm excursion.

PubMed

Goutman, Stephen A; Hamilton, James D; Swihart, Blake; Foerster, Bradley; Feldman, Eva L; Rubin, Jonathan M

2017-01-01

Diaphragm excursion measured via ultrasound may be an important imaging outcome measure of respiratory function. We developed a new method for measuring diaphragm movement and compared it to the more traditional M-mode method. Ultrasound images of the right and left hemidiaphragms were collected to compare speckle tracking and M-mode measurements of diaphragm excursion. Speckle tracking was performed using EchoInsight (Epsilon Imaging, Ann Arbor, Michigan). Six healthy subjects without a history of pulmonary diseases were included in this proof-of-concept study. Speckle tracking of the diaphragm is technically possible. Unlike M-mode, speckle tracking carries the advantage of reliable visualization and measurement of the left hemidiaphragm. Speckle tracking accounted for diaphragm movement simultaneously in the cephalocaudad and mediolateral directions, unlike M-mode, which is 1-dimensional. Diaphragm speckle tracking may represent a novel, more robust method for measuring diaphragm excursion, especially for the left hemidiaphragm. Muscle Nerve 55: 125-127, 2017. © 2016 Wiley Periodicals, Inc.

Comparison of linear cephalometric dimensions in Americans of European descent (Ann Arbor, Cleveland, Philadelphia) and Americans of African descent (Nashville).

PubMed

Dibbets, Jos M H; Nolte, Kai

2002-08-01

Eleven dimensions, extracted from four commercially available cephalometric atlases were compared. Three populations were American of European descent and one was American of African descent. The source data were carefully corrected for linear enlargement. The confounding effect of linear radiographic enlargement is exemplified by depicting the often-used distance, sella-nasion, before and after correction. Total face height was smallest in the Cleveland population and largest in the Nashville population. The difference was fully accounted for by differences in lower face height and that was the most variable of all dimensions studied. Upper face height was almost identical in all four populations. Posterior face height was largest in the Nashville population. The mandible in the Nashville population had an average ramus height, but a longer corpus. Mandibular dimensions were equal in the three other populations. The maxilla was clearly shortest in the Cleveland population and almost of equal length in the three others.

Remote sensing of changes in morphology and physiology of trees under stress. [for detecting Fomes annosus

NASA Technical Reports Server (NTRS)

Olson, C. F., Jr.

1972-01-01

Previsual detection of Fomes annosus in pine plantations was studied. Detailed analyses of photographic imagery obtained over the Ann Arbor Test Site during 1969 and 1970 reveal that the Ektachrome Infrared film was superior to Ektachrome Aerographic, Infrared Aerographic, or Plus-X Aerographic films for detecting Fomes annosus damage. Of far more significance in controlling the accuracy of damage detection, however, was the experience of the photo interpreter. Ratio-processing of multispectral scanner data was investigated with data collected in June of 1970 and in June of 1972. Ratioing of the 1.5-1.8 and 1.0-1.4 micrometer channels gave good results at detecting openings in the crown canopy and adjacent infected trees. Combined level slicing of the 1.5-1.8 micrometer channel and the 1.5-1.8 to 1.0-1.4 micrometer ratio permitted separation and recognition of forest litter in the openings and stressed trees adjacent ot the openings.

Industry's tireless eyes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1987-08-01

This article reports that there are literally hundreds of machine vision systems from which to choose. They range in cost from $10,000 to $1,000,000. Most have been designed for specific applications; the same systems if used for a different application may fail dismally. How can you avoid wasting money on inferior, useless, or nonexpandable systems. A good reference is the Automated Vision Association in Ann Arbor, Mich., a trade group comprised of North American machine vision manufacturers. Reputable suppliers caution users to do their homework before making an investment. Important considerations include comprehensive details on the objects to be viewed-thatmore » is, quantity, shape, dimension, size, and configuration details; lighting characteristics and variations; component orientation details. Then, what do you expect the system to do-inspect, locate components, aid in robotic vision. Other criteria include system speed and related accuracy and reliability. What are the projected benefits and system paybacks.. Examine primarily paybacks associated with scrap and rework reduction as well as reduced warranty costs.« less

A valiant little terminal: A VLT user's manual

DOE Office of Scientific and Technical Information (OSTI.GOV)

Weinstein, A.

1992-08-01

VLT came to be used at SLAC (Stanford Linear Accelerator Center), because SLAC wanted to assess the Amiga's usefulness as a color graphics terminal and T{sub E}X workstation. Before the project could really begin, the people at SLAC needed a terminal emulator which could successfully talk to the IBM 3081 (now the IBM ES9000-580) and all the VAXes on the site. Moreover, it had to compete in quality with the Ann Arbor Ambassador GXL terminals which were already in use at the laboratory. Unfortunately, at the time there was no commercial program which fit the bill. Luckily, Willy Langeveld hadmore » been independently hacking up a public domain VT100 emulator written by Dave Wecker et al. and the result, VLT, suited SLAC's purpose. Over the years, as the program was debugged and rewritten, the original code disappeared, so that now, in the present version of VLT, none of the original VT100 code remains.« less

A valiant little terminal: A VLT user`s manual. Revision 4

DOE Office of Scientific and Technical Information (OSTI.GOV)

Weinstein, A.

1992-08-01

VLT came to be used at SLAC (Stanford Linear Accelerator Center), because SLAC wanted to assess the Amiga`s usefulness as a color graphics terminal and T{sub E}X workstation. Before the project could really begin, the people at SLAC needed a terminal emulator which could successfully talk to the IBM 3081 (now the IBM ES9000-580) and all the VAXes on the site. Moreover, it had to compete in quality with the Ann Arbor Ambassador GXL terminals which were already in use at the laboratory. Unfortunately, at the time there was no commercial program which fit the bill. Luckily, Willy Langeveld hadmore » been independently hacking up a public domain VT100 emulator written by Dave Wecker et al. and the result, VLT, suited SLAC`s purpose. Over the years, as the program was debugged and rewritten, the original code disappeared, so that now, in the present version of VLT, none of the original VT100 code remains.« less

Strategic Planning as a Tool for Achieving Alignment in Academic Health Centers

PubMed Central

Higginbotham, Eve J.; Church, Kathryn C.

2012-01-01

After the passage of the Patient Protection and Affordable Care Act in March 2010, there is an urgent need for medical schools, teaching hospitals, and practice plans to work together seamlessly across a common mission. Although there is agreement that there should be greater coordination of initiatives and resources, there is little guidance in the literature to address the method to achieve the necessary transformation. Traditional approaches to strategic planning often engage a few leaders and produce a set of immeasurable initiatives. A nontraditional approach, consisting of a Whole-Scale (Dannemiller Tyson Associates, Ann Arbor, MI) engagement, appreciative inquiry, and a balanced scorecard can, more rapidly transform an academic health center. Using this nontraditional approach to strategic planning, increased organizational awareness was achieved in a single academic health center. Strategic planning can be an effective tool to achieve alignment, enhance accountability, and a first step in meeting the demands of the new landscape of healthcare. PMID:23303997

Meeting report for "OddPols" 2014: the odds invite an even.

PubMed

Roy-Engel, Astrid M

2015-02-01

The Ninth International Biennial Conference on RNA Polymerases I and III (the "OddPols") was held on June 19-21, 2014 at the University of Michigan, Ann Arbor, USA. Sponsored by New England Biolabs, the Cayman Chemical Company, the Rackham Graduate School and the University of Michigan Health System, and organized by David Engelke, Craig Pikaard, Lawrence Rothblum, Andrzej Wierzbicki and Astrid Engel. This year at the conference, the "odds" were increased by expanding the usual topics on the advances in RNA polymerases I and III research to include presentations on RNA polymerase IV and V. The keynote speaker, Craig Pikaard, opened the meeting with his presentation entitled "Five nuclear multisubunit RNA polymerases". The meeting drew attendees from fourteen countries that shared their research discoveries through oral and poster presentations. The talks were organized into 11 sessions covering seven distinct topics. Here we present some of the highlights from the meeting using summaries provided by the participants. Copyright © 2014 Elsevier B.V. All rights reserved.

Concurrent colonic mucosa-associated lymphoid tissue lymphoma and adenoma diagnosed after a positive fecal occult blood test: a case report.

PubMed

Lin, Pei-Chiang; Chen, Jinn-Shiun; Deng, Po; Wang, Chih-Wei; Huang, Chiung-Huei; Tang, Reiping; Chiang, Jy-Ming; Yeh, Chien-Yuh; Hsieh, Pao-Shiu; Tsai, Wen-Sy; Chiang, Sum-Fu

2016-01-27

Colonic lymphoma is an uncommon presentation of extranodal lymphoma. Colonic mucosa-associated lymphoid tissue lymphoma is a different entity from gastric mucosa-associated lymphoid tissue lymphoma, and very rare. The presentation and management of colonic mucosa-associated lymphoid tissue are highly variable in the literature. We report the case of a 59-year-old Taiwanese man who underwent a colonoscopy after a positive test for fecal occult blood. His past history included hypertension and hyperthyroidism. The colonoscopy revealed an adenomatous polyp and mucosa-associated lymphoid tissue lymphoma. We successfully performed a polypectomy and endoscopic mucosal resection. The lymphoma was staged according to the Ann Arbor system modified by Musshoff as E-I. Our patient showed no lymphoma recurrence over a 3-year follow-up. Endoscopic mucosal resection for colonic mucosa-associated lymphoid tissue lymphoma without disseminated disease may be feasible. We successfully used colonoscopic treatment without adjuvant therapy to treat early-stage pathogen-free colonic mucosa-associated lymphoid tissue lymphoma.

Primary gastric mucosa associated lymphoid tissue lymphoma: Clinical data predicted treatment outcome

PubMed Central

Todorovic, Milena; Balint, Bela; Jevtic, Miodrag; Suvajdzic, Nada; Ceric, Amela; Stamatovic, Dragana; Markovic, Olivera; Perunicic, Maja; Marjanovic, Slobodan; Krstic, Miodrag

2008-01-01

AIM: To determine clinical characteristics and treatment outcome of gastric lymphoma after chemotherapy and immuno-chemotherapy. METHODS: Thirty four patients with primary gastric mucosa associated lymphoid tissue (MALT) lymphoma (Ann Arbor stages I to IV) were enrolled. All had upper gastric endoscopy, abdominal ultrasonography, CT and H pylori status assessment (histology and serology). After anti-H pylori treatment and initial chemotherapy, patients were re-examined every 4 mo. RESULTS: Histological regression of the lymphoma was complete in 22/34 (64.7%) and partial in 9 (26.5%) patients. Median follow up time for these 31 responders was 60 mo (range 48-120). No regression was noted in 3 patients. Among the 25 (73.5%) H pylori positive patients, the eradication rate was 100%. CONCLUSION: Using univariate analysis, predictive factors for overall survival were international prognostic index (IPI) score, hemoglobin level, erythrocyte sedimentation rate (ESR), and platelet numbers (P < 0.005). In addition to this, Cox proportion hazard model differentiate IPI score, ESR, and platelets as predictors of survival. PMID:18416467

International Symposium on Remote Sensing of Environment, 17th, University of Michigan, Ann Arbor, MI, May 9-13, 1983, Proceedings. Volumes 1, 2 & 3

NASA Technical Reports Server (NTRS)

1984-01-01

The collection, processing, and analysis of remote-sensing data from ground-based, airborne, and spaceborne instruments for application to the monitoring and management of the earth and environment and resources are examined in reviews and reports, some in summary form. Subject areas covered include US policy and directions on remote sensing (RS); the future of terrestrial RS from space; RS of land, oceans, and atmosphere from a global perspective; RS in hydrological modeling; microprocessing technology; array processors; geobased information systems; artificial intelligence; the Shuttle imaging radar; and current results from Landsat-4. Among the specific topics discussed are RS application to hydrocarbon exploration, airborne gamma-radiation assessment of snow water equivalent, surface-vegetation-biomass modeling from AVHRR and Landsat data, Landsat imagery of Mediterranean pollution, fast two-dimensional filtering of thermal-scanner data, RS of severe convective storms, registration of rotated images by invariant moments, and the geometric accuracy of Landsat-4 Thematic-Mapper P-tapes.

Tethers in Space

NASA Astrophysics Data System (ADS)

Johnson, Les; Bilén, Sven G.; Gilchrist, Brian E.; Krause, Linda Habash

2017-09-01

This Special Section of Acta Astronautica contains several peer-reviewed papers selected from among those presented at the Fifth International Conference on Tethers in Space (TiS2016). After a hiatus of 21 years since the last Conference on Tethers in Space, TiS2016 brought together experts, practitioners, and other interested in space tethers and related fields. TiS2016 was held May 24-26, 2016 at The University of Michigan in Ann Arbor, Michigan, U.S.A. Leveraging the hard work of a great many volunteers, the conference co-chairs Prof. Brian Gilchrist and Prof. Sven Bilén welcomed an international contingent with authors from the U.S., Canada, Japan, Spain, China, Finland, Estonia, and Italy. The community provided updates on what has transpired since their last gathering by providing lessons learned; describing new technologies and subsystems; and proposing new tether missions and applications. Papers overviewed de-orbit systems, power generation, orbital maneuvering, momentum capture, debris removal, space tugs, space elevators, collision avoidance, and tether dynamics, among others.

Alternate Splicing of CD44 Messenger RNA in Prostate Cancer Growth

DTIC Science & Technology

2008-04-01

hammerhead ribozymes .25 Salmon CT (BAChem, Torrance, CA) was used at physiologic 50 nM dose14,16, which effectively alters CD44,6 or at 250 nM. To detect...receptor14), and cells called CTR-, derived from PC-3M cells after anti-CT receptor ribozyme knockdown of CTR.18 CTR- cells have very low levels of

Electrically Stimulated Antagonist Muscle Contraction Increased Muscle Mass and Bone Mineral Density of One Astronaut - Initial Verification on the International Space Station.

PubMed

Shiba, Naoto; Matsuse, Hiroo; Takano, Yoshio; Yoshimitsu, Kazuhiro; Omoto, Masayuki; Hashida, Ryuki; Tagawa, Yoshihiko; Inada, Tomohisa; Yamada, Shin; Ohshima, Hiroshi

2015-01-01

Musculoskeletal atrophy is one of the major problems of extended periods of exposure to weightlessness such as on the International Space Station (ISS). We developed the Hybrid Training System (HTS) to maintain an astronaut's musculoskeletal system using an electrically stimulated antagonist to resist the volitional contraction of the agonist instead of gravity. The present study assessed the system's orbital operation capability and utility, as well as its preventative effect on an astronaut's musculoskeletal atrophy. HTS was attached to the non-dominant arm of an astronaut staying on the ISS, and his dominant arm without HTS was established as the control (CTR). 10 sets of 10 reciprocal elbow curls were one training session, and 12 total sessions of training (3 times per week for 4 weeks) were performed. Pre and post flight ground based evaluations were performed by Biodex (muscle performance), MRI (muscle volume), and DXA (BMD, lean [muscle] mass, fat mass). Pre and post training inflight evaluations were performed by a hand held dynamometer (muscle force) and a measuring tape (upper arm circumference). The experiment was completed on schedule, and HTS functioned well without problems. Isokinetic elbow extension torque (Nm) changed -19.4% in HTS, and -21.7% in CTR. Isokinetic elbow flexion torque changed -23.7% in HTS, and there was no change in CTR. Total Work (Joule) of elbow extension changed -8.3% in HTS, and +0.3% in CTR. For elbow flexion it changed -23.3% in HTS and -32.6% in CTR. Average Power (Watts) of elbow extension changed +22.1% in HTS and -8.0% in CTR. For elbow flexion it changed -6.5% in HTS and -4.8% in CTR. Triceps muscle volume according to MRI changed +11.7% and that of biceps was +2.1% using HTS, however -0.1% and -0.4% respectively for CTR. BMD changed +4.6% in the HTS arm and -1.2% for CTR. Lean (muscle) mass of the arm changed only +10.6% in HTS. Fat mass changed -12.6% in HTS and -6.4% in CTR. These results showed the orbital operation capability and utility, and the preventive effect of HTS for an astronaut's musculoskeletal atrophy. The initial flight data together with the ground data obtained so far will be utilized in the future planning of human space exploration.

Association between serum aluminum levels and cardiothoracic ratio in patients on chronic hemodialysis

PubMed Central

Wang, Tzu-Lin; Fang, Yu-Wei; Leu, Jyh-Gang

2017-01-01

The cardiothoracic ratio (CTR) and serum aluminum levels are both associated with mortality in hemodialysis patients. However, limited data regarding the association between serum aluminum levels and the CTR have been published to date. Therefore, we aimed to elucidate this association in patients on chronic hemodialysis (CHD). We investigated the association between the serum aluminum level and the CTR in CHD in a retrospective cross-sectional study of 547 Taiwanese patients on CHD. The mean age of patients was 62.5±13.2 years, with a mean hemodialysis time of 7.1±5.2 years. Among the patients, 36.9% were diabetic and 47.9% were male. After natural logarithmic transformation (ln(aluminum)), the serum aluminum level exhibited an independent and linear relationship with the CTR (β: 1.40, 95% confidence interval (CI), 0.6–2.2). A high serum aluminum level (≥6 ng/dL) was significantly associated with a CTR >0.5 in the crude analysis (odds ratio (OR): 2.15, 95% CI, 1.52–3.04) and remained significant after multivariable adjustment (OR: 2.45, 95% CI, 1.63–3.67). Moreover, the ln(aluminum) value was significantly associated with a CTR >0.5 (OR: 1.71, 95%CI, 1.28–2.29) in multivariable analysis, indicating a dose effect of aluminum on cardiomegaly. In conclusion, the serum aluminum level was independently associated with cardiac remodeling (elevated CTR) in patients on CHD. PMID:29261793

Platinum transfer from hCTR1 to Atox1 is dependent on the type of platinum complex.

PubMed

Wu, Xuelei; Yuan, Siming; Wang, Erqiong; Tong, Yang; Ma, Guolin; Wei, Kaiju; Liu, Yangzhong

2017-05-24

In spite of their wide application, the cellular uptake of platinum based anticancer drugs is still unclear. The copper transport protein, hCTR1, is proposed to facilitate the cellular uptake of cisplatin, whereas organic cation transport (OCT) is more important for oxaliplatin. It has been reported that both N-terminal and C-terminal metal binding motifs of hCTR1 are highly reactive to cisplatin, which is the initial step of protein assisted cellular uptake of cisplatin. It is still unknown how the platinum drugs in hCTR1 transfer to cytoplasmic media, and whether various platinum complexes possess different activities in this process. Herein, we investigated the reaction of the platinated C-terminal metal binding motif of hCTR1 (C8) with the down-stream protein Atox1. Results show that Atox1 is highly reactive to the platinated C8 adducts of cisplatin and transplatin, whereas the oxaliplatin/C8 adduct is much less reactive. The platinum transfer from C8 to Atox1 occurs in the reaction, which results in the protein unfolding of Atox1. These results demonstrated that the platinated intracellular-domain of hCTR1 is reactive to Atox1, and the reactivity is dependent on the ligand and the coordination structure of platinum complexes. The different reactivity is consistent with the hypothesis that hCTR1 is more significant in the transport of cisplatin than that of oxaliplatin.

Characterizing the Spatial Density Functions of Neural Arbors

NASA Astrophysics Data System (ADS)

Teeter, Corinne Michelle

Recently, it has been proposed that a universal function describes the way in which all arbors (axons and dendrites) spread their branches over space. Data from fish retinal ganglion cells as well as cortical and hippocampal arbors from mouse, rat, cat, monkey and human provide evidence that all arbor density functions (adf) can be described by a Gaussian function truncated at approximately two standard deviations. A Gaussian density function implies that there is a minimal set of parameters needed to describe an adf: two or three standard deviations (depending on the dimensionality of the arbor) and an amplitude. However, the parameters needed to completely describe an adf could be further constrained by a scaling law found between the product of the standard deviations and the amplitude of the function. In the following document, I examine the scaling law relationship in order to determine the minimal set of parameters needed to describe an adf. First, I find that the at, two-dimensional arbors of fish retinal ganglion cells require only two out of the three fundamental parameters to completely describe their density functions. Second, the three-dimensional, volume filling, cortical arbors require four fundamental parameters: three standard deviations and the total length of an arbor (which corresponds to the amplitude of the function). Next, I characterize the shape of arbors in the context of the fundamental parameters. I show that the parameter distributions of the fish retinal ganglion cells are largely homogenous. In general, axons are bigger and less dense than dendrites; however, they are similarly shaped. The parameter distributions of these two arbor types overlap and, therefore, can only be differentiated from one another probabilistically based on their adfs. Despite artifacts in the cortical arbor data, different types of arbors (apical dendrites, non-apical dendrites, and axons) can generally be differentiated based on their adfs. In addition, within arbor type, there is evidence of different neuron classes (such as interneurons and pyramidal cells). How well different types and classes of arbors can be differentiated is quantified using the Random ForestTM supervised learning algorithm.

In vitro antibacterial action of Tetraclean, MTAD and five experimental irrigation solutions.

PubMed

Pappen, F G; Shen, Y; Qian, W; Leonardo, M R; Giardino, L; Haapasalo, M

2010-06-01

To investigate the antibacterial effect of Tetraclean, MTAD and five experimental irrigants using both direct exposure test with planktonic cultures and mixed-species in vitro biofilm model. Tetraclean, MTAD and five experimental solutions that were modifications of existing formulae including MTAD + 0.01% cetrimide (CTR), MTAD + 0.1% CTR, MTAC-1 (Tween 80 replaced by 0.01% CTR in MTAD), MTAC-2 (Tween 80 replaced by 0.1% CTR) and MTAD-D (MTAD without the Tween 80 and no CTR added) were used as disinfectants in the experiments. In the direct exposure test, a suspension of Enterococcus faecalis was mixed with each of the solutions. After 0.5, 1, 3 and 10 min, an inactivator was added and the number of surviving bacteria was calculated. A mixed-species biofilm from subgingival plaque bacteria was grown in brain heart infusion broth in anaerobic conditions on synthetic hydroxyapatite discs. Two-week-old biofilms were exposed to the solutions for 0.5, 1 and 3 min. The samples were observed by confocal laser scanning microscopy after bacterial viability staining. The scans were quantitatively analysed, and the volume of killed cells of all cells was calculated for each medicament. Tetraclean and MTAC-2 (0.1% CTR) killed planktonic E. faecalis in <30 s. Complete killing of bacteria required 1 min by MTAC-1, 3 min by MTAD + 0.1% CTR and 10 min by MTAD, MTAD-D and MTAD + 0.01% CTR. In the biofilm test, there were significant differences in microbial killing between the different solutions and times of exposure (P < 0.005). MTAC-2 showed the best performance, killing 71% of the biofilm bacteria in 3 min, followed by MTAC-1 and Tetraclean. MTAD and the three MTAD modifications demonstrated the lowest antibacterial activity. Tetraclean was more effective than MTAD against E. faecalis in planktonic culture and in mixed-species in vitro biofilm. CTR improved the antimicrobial properties of the solutions, whereas Tween 80 seemed to have a neutral or negative impact on their antimicrobial effectiveness.

MULTIMODAL CLASSIFICATION OF DEMENTIA USING FUNCTIONAL DATA, ANATOMICAL FEATURES AND 3D INVARIANT SHAPE DESCRIPTORS

PubMed Central

Mikhno, Arthur; Nuevo, Pablo Martinez; Devanand, Davangere P.; Parsey, Ramin V.; Laine, Andrew F.

2013-01-01

Multimodality classification of Alzheimer’s disease (AD) and its prodromal stage, Mild Cognitive Impairment (MCI), is of interest to the medical community. We improve on prior classification frameworks by incorporating multiple features from MRI and PET data obtained with multiple radioligands, fluorodeoxyglucose (FDG) and Pittsburg compound B (PIB). We also introduce a new MRI feature, invariant shape descriptors based on 3D Zernike moments applied to the hippocampus region. Classification performance is evaluated on data from 17 healthy controls (CTR), 22 MCI, and 17 AD subjects. Zernike significantly outperforms volume, accuracy (Zernike to volume): CTR/AD (90.7% to 71.6%), CTR/MCI (76.2% to 60.0%), MCI/AD (84.3% to 65.5%). Zernike also provides comparable and complementary performance to PET. Optimal accuracy is achieved when Zernike and PET features are combined (accuracy, specificity, sensitivity), CTR/AD (98.8%, 99.5%, 98.1%), CTR/MCI (84.3%, 82.9%, 85.9%) and MCI/AD (93.3%, 93.6%, 93.3%). PMID:24576927

MULTIMODAL CLASSIFICATION OF DEMENTIA USING FUNCTIONAL DATA, ANATOMICAL FEATURES AND 3D INVARIANT SHAPE DESCRIPTORS.

PubMed

Mikhno, Arthur; Nuevo, Pablo Martinez; Devanand, Davangere P; Parsey, Ramin V; Laine, Andrew F

2012-01-01

Multimodality classification of Alzheimer's disease (AD) and its prodromal stage, Mild Cognitive Impairment (MCI), is of interest to the medical community. We improve on prior classification frameworks by incorporating multiple features from MRI and PET data obtained with multiple radioligands, fluorodeoxyglucose (FDG) and Pittsburg compound B (PIB). We also introduce a new MRI feature, invariant shape descriptors based on 3D Zernike moments applied to the hippocampus region. Classification performance is evaluated on data from 17 healthy controls (CTR), 22 MCI, and 17 AD subjects. Zernike significantly outperforms volume, accuracy (Zernike to volume): CTR/AD (90.7% to 71.6%), CTR/MCI (76.2% to 60.0%), MCI/AD (84.3% to 65.5%). Zernike also provides comparable and complementary performance to PET. Optimal accuracy is achieved when Zernike and PET features are combined (accuracy, specificity, sensitivity), CTR/AD (98.8%, 99.5%, 98.1%), CTR/MCI (84.3%, 82.9%, 85.9%) and MCI/AD (93.3%, 93.6%, 93.3%).

Functional characterization of the copper transcription factor AfMac1 from Aspergillus fumigatus.

PubMed

Park, Yong-Sung; Kim, Tae-Hyoung; Yun, Cheol-Won

2017-07-03

Although copper functions as a cofactor in many physiological processes, copper overload leads to harmful effects in living cells. Thus, copper homeostasis is tightly regulated. However, detailed copper metabolic pathways have not yet been identified in filamentous fungi. In this report, we investigated the copper transcription factor AfMac1 ( A spergillus f umigatus Mac1 homolog) and identified its regulatory mechanism in A. fumigatus AfMac1 has domains homologous to the DNA-binding and copper-binding domains of Mac1 from Saccharomyces cerevisiae , and AfMac1 efficiently complemented Mac1 in S. cerevisiae Expression of Afmac1 resulted in CTR1 up-regulation, and mutation of the DNA-binding domain of Afmac1 failed to activate CTR1 expression in S. cerevisiae The Afmac1 deletion strain of A. fumigatus failed to grow in copper-limited media, and its growth was restored by introducing ctrC We found that AfMac1 specifically bound to the promoter region of ctrC based on EMSA. The AfMac1-binding motif 5'-TGTGCTCA-3' was identified from the promoter region of ctrC , and the addition of mutant ctrC lacking the AfMac1-binding motif failed to up-regulate ctrC in A. fumigatus Furthermore, deletion of Afmac1 significantly reduced strain virulence and activated conidial killing activity by neutrophils and macrophages. Taken together, these results suggest that AfMac1 is a copper transcription factor that regulates cellular copper homeostasis in A. fumigatus . © 2017 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society.

Electrically Stimulated Antagonist Muscle Contraction Increased Muscle Mass and Bone Mineral Density of One Astronaut - Initial Verification on the International Space Station

PubMed Central

Takano, Yoshio; Yoshimitsu, Kazuhiro; Omoto, Masayuki; Hashida, Ryuki; Tagawa, Yoshihiko; Inada, Tomohisa; Yamada, Shin; Ohshima, Hiroshi

2015-01-01

Background Musculoskeletal atrophy is one of the major problems of extended periods of exposure to weightlessness such as on the International Space Station (ISS). We developed the Hybrid Training System (HTS) to maintain an astronaut’s musculoskeletal system using an electrically stimulated antagonist to resist the volitional contraction of the agonist instead of gravity. The present study assessed the system’s orbital operation capability and utility, as well as its preventative effect on an astronaut’s musculoskeletal atrophy. Methods HTS was attached to the non-dominant arm of an astronaut staying on the ISS, and his dominant arm without HTS was established as the control (CTR). 10 sets of 10 reciprocal elbow curls were one training session, and 12 total sessions of training (3 times per week for 4 weeks) were performed. Pre and post flight ground based evaluations were performed by Biodex (muscle performance), MRI (muscle volume), and DXA (BMD, lean [muscle] mass, fat mass). Pre and post training inflight evaluations were performed by a hand held dynamometer (muscle force) and a measuring tape (upper arm circumference). Results The experiment was completed on schedule, and HTS functioned well without problems. Isokinetic elbow extension torque (Nm) changed -19.4% in HTS, and -21.7% in CTR. Isokinetic elbow flexion torque changed -23.7% in HTS, and there was no change in CTR. Total Work (Joule) of elbow extension changed -8.3% in HTS, and +0.3% in CTR. For elbow flexion it changed -23.3% in HTS and -32.6% in CTR. Average Power (Watts) of elbow extension changed +22.1% in HTS and -8.0% in CTR. For elbow flexion it changed -6.5% in HTS and -4.8% in CTR. Triceps muscle volume according to MRI changed +11.7% and that of biceps was +2.1% using HTS, however -0.1% and -0.4% respectively for CTR. BMD changed +4.6% in the HTS arm and -1.2% for CTR. Lean (muscle) mass of the arm changed only +10.6% in HTS. Fat mass changed -12.6% in HTS and -6.4% in CTR. Conclusions These results showed the orbital operation capability and utility, and the preventive effect of HTS for an astronaut’s musculoskeletal atrophy. The initial flight data together with the ground data obtained so far will be utilized in the future planning of human space exploration. PMID:26296204

SLC31 (CTR) Family of Copper Transporters in Health and Disease

PubMed Central

Kim, Heejeong; Wu, Xiaobin; Lee, Jaekwon

2012-01-01

Copper is a vital mineral for many organisms, yet it is highly toxic as demonstrated by serious health concerns associated with its deficiency or excess accumulation. The SLC31 (CTR) family of copper transporters is a major gateway of copper acquisition in eukaryotes, ranging from yeast to humans. Characterization of the function, modes of action, and regulation of CTR and other molecular factors that functionally cooperate with CTR for copper transport, compartmentalization, incorporation into cuproproteins, and detoxification has revealed that organisms have evolved fascinating mechanisms for tight control of copper metabolism. This research progress further indicates the significance of copper in health and disease and opens avenues for therapeutic control of copper bioavailability and its metabolic pathways. PMID:23506889

Review of the registration of clinical trials in UMIN-CTR from 2 June 2005 to 1 June 2010 - focus on Japan domestic, academic clinical trials

PubMed Central

2013-01-01

Background Established on 1 June 2005, the University Hospital Medical Information Network Clinical Trials Registry (UMIN-CTR) is the largest clinical trial registry in Japan, and joined the World Health Organization (WHO) registry network in October 2008. Our aim was to understand the registration trend and overall characteristics of Japan domestic, academic (non-industry-funded) clinical trials, which constitute the main body of registrations in UMIN-CTR. In addition, we aimed to investigate the accessibility of clinical trials in UMIN-CTR to people worldwide, as well as the accessibility of clinical trials conducted in Japan but registered abroad to Japanese people in the Japanese language. Methods We obtained the data for registrations in UMIN-CTR from the UMIN Center, and extracted Japan domestic, academic clinical trials to analyze their registration trend and overall characteristics. We also investigated how many of the trials registered in UMIN-CTR could be accessed from the International Clinical Trials Registry Platform (ICTRP). Finally, we searched ClinicalTrials.gov for all clinical trials conducted in Japan and investigated how many of them were also registered in Japanese registries. All of the above analyses included clinical trials registered from 2 June 2005 to 1 June 2010. Results During the period examined, the registration trend showed an obvious peak around September 2005 and rapid growth from April 2009. Of the registered trials, 46.4% adopted a single-arm design, 34.5% used an active control, only 10.9% were disclosed before trial commencement, and 90.0% did not publish any results. Overall, 3,063 of 3,064 clinical trials registered in UMIN-CTR could be accessed from ICTRP. Only 8.7% of all clinical trials conducted in Japan and registered in ClinicalTrials.gov were also registered in Japanese registries. Conclusions The International Committee of Medical Journal Editors (ICMJE) announcements about clinical trial registration and the Ethical Guidelines for Clinical Research published by the Japanese government are considered to have promoted clinical trial registration in UMIN-CTR. However, problems associated with trial design, retrospective registration, and publication of trial results need to be addressed in future. Almost all clinical trials registered in UMIN-CTR are accessible to people worldwide through ICTRP. However, many trials conducted in Japan but registered abroad cannot be accessed from Japanese registries in Japanese. PMID:24124926

Clinical and optical intraocular performance of rotationally asymmetric multifocal IOL plate-haptic design versus C-loop haptic design.

PubMed

Alió, Jorge L; Plaza-Puche, Ana B; Javaloy, Jaime; Ayala, María José; Vega-Estrada, Alfredo

2013-04-01

To compare the visual and intraocular optical quality outcomes with different designs of the refractive rotationally asymmetric multifocal intraocular lens (MFIOL) (Lentis Mplus; Oculentis GmbH, Berlin, Germany) with or without capsular tension ring (CTR) implantation. One hundred thirty-five consecutive eyes of 78 patients with cataract (ages 36 to 82 years) were divided into three groups: 43 eyes implanted with the C-Loop haptic design without CTR (C-Loop haptic only group); 47 eyes implanted with the C-Loop haptic design with CTR (C-Loop haptic with CTR group); and 45 eyes implanted with the plate-haptic design (plate-haptic group). Visual acuity, contrast sensitivity, defocus curve, and ocular and intraocular optical quality were evaluated at 3 months postoperatively. Significant differences in the postoperative sphere were found (P = .01), with a more myopic postoperative refraction for the C-Loop haptic only group. No significant differences were detected in photopic and scotopic contrast sensitivity among groups (P ⩾ .05). Significantly better visual acuities were present in the C-Loop haptic with CTR group for the defocus levels of -2.0, -1.5, -1.0, and -0.50 D (P ⩽.03). Statistically significant differences among groups were found in total intraocular root mean square (RMS), high-order intraocular RMS, and intraocular coma-like RMS aberrations (P ⩽.04), with lower values from the plate-haptic group. The plate-haptic design and the C-Loop haptic design with CTR implantation both allow good visual rehabilitation. However, better refractive predictability and intraocular optical quality was obtained with the plate-haptic design without CTR implantation. The plate-haptic design seems to be a better design to support rotational asymmetric MFIOL optics. Copyright 2013, SLACK Incorporated.

Improving clinical and translational research training: a qualitative evaluation of the Atlanta Clinical and Translational Science Institute KL2-mentored research scholars program

PubMed Central

Comeau, Dawn L; Escoffery, Cam; Freedman, Ariela; Ziegler, Thomas R; Blumberg, Henry M

2017-01-01

A major impediment to improving the health of communities is the lack of qualified clinical and translational research (CTR) investigators. To address this workforce shortage, the National Institutes of Health (NIH) developed mechanisms to enhance the career development of CTR physician, PhD, and other doctoral junior faculty scientists including the CTR-focused K12 program and, subsequently, the KL2-mentored CTR career development program supported through the Clinical and Translational Science Awards (CTSAs). Our evaluation explores the impact of the K12/KL2 program embedded within the Atlanta Clinical and Translational Science Institute (ACTSI), a consortium linking Emory University, Morehouse School of Medicine and the Georgia Institute of Technology. We conducted qualitative interviews with program participants to evaluate the impact of the program on career development and collected data on traditional metrics (number of grants, publications). 46 combined K12/KL2 scholars were supported between 2002 and 2016. 30 (65%) of the 46 K12/KL2 scholars are women; 24 (52%) of the trainees are minorities, including 10 (22%) scholars who are members of an underrepresented minority group. Scholars reported increased research skills, strong mentorship experiences, and positive impact on their career trajectory. Among the 43 scholars who have completed the program, 39 (91%) remain engaged in CTR and received over $89 000 000 as principal investigators on federally funded awards. The K12/KL2 funding provided the training and protected time for successful career development of CTR scientists. These data highlight the need for continued support for CTR training programs for junior faculty. PMID:27591319

Copper transporters and chaperones CTR1, CTR2, ATOX1, and CCS as determinants of cisplatin sensitivity.

PubMed

Bompiani, Kristin M; Tsai, Cheng-Yu; Achatz, Felix P; Liebig, Janika K; Howell, Stephen B

2016-09-01

The development of resistance to cisplatin (cDDP) is commonly accompanied by reduced drug uptake or increased efflux. Previous studies in yeast and murine embryonic fibroblasts have reported that the copper (Cu) transporters and chaperones participate in the uptake, efflux, and intracellular distribution of cDDP. However, there is conflicting data from studies in human cells. We used CRISPR-Cas9 genome editing to individually knock out the human copper transporters CTR1 and CTR2 and the copper chaperones ATOX1 and CCS. Isogenic knockout cell lines were generated in both human HEK-293T and ovarian carcinoma OVCAR8 cells. All knockout cell lines had slowed growth compared to parental cells, small changes in basal Cu levels, and varying sensitivities to Cu depending on the gene targeted. However, all of the knockouts demonstrated only modest 2 to 5-fold changes in cDDP sensitivity that did not differ from the range of sensitivities of 10 wild type clones grown from the same parental cell population. We conclude that, under basal conditions, loss of CTR1, CTR2, ATOX1, or CCS does not produce a change in cisplatin sensitivity that exceeds the variance found within the parental population, suggesting that they are not essential to the mechanism by which cDDP enters these cell lines and is transported to the nucleus.

Single TRAM domain RNA-binding proteins in Archaea: functional insight from Ctr3 from the Antarctic methanogen Methanococcoides burtonii.

PubMed

Taha; Siddiqui, K S; Campanaro, S; Najnin, T; Deshpande, N; Williams, T J; Aldrich-Wright, J; Wilkins, M; Curmi, P M G; Cavicchioli, R

2016-09-01

TRAM domain proteins present in Archaea and Bacteria have a β-barrel shape with anti-parallel β-sheets that form a nucleic acid binding surface; a structure also present in cold shock proteins (Csps). Aside from protein structures, experimental data defining the function of TRAM domains is lacking. Here, we explore the possible functional properties of a single TRAM domain protein, Ctr3 (cold-responsive TRAM domain protein 3) from the Antarctic archaeon Methanococcoides burtonii that has increased abundance during low temperature growth. Ribonucleic acid (RNA) bound by Ctr3 in vitro was determined using RNA-seq. Ctr3-bound M. burtonii RNA with a preference for transfer (t)RNA and 5S ribosomal RNA, and a potential binding motif was identified. In tRNA, the motif represented the C loop; a region that is conserved in tRNA from all domains of life and appears to be solvent exposed, potentially providing access for Ctr3 to bind. Ctr3 and Csps are structurally similar and are both inferred to function in low temperature translation. The broad representation of single TRAM domain proteins within Archaea compared with their apparent absence in Bacteria, and scarcity of Csps in Archaea but prevalence in Bacteria, suggests they represent distinct evolutionary lineages of functionally equivalent RNA-binding proteins. © 2016 Society for Applied Microbiology and John Wiley & Sons Ltd.

Novel process for production of micro lenses with increased centering accuracy and imaging performance

NASA Astrophysics Data System (ADS)

Wilde, C.; Langehanenberg, P.; Schenk, T.

2017-10-01

For modern production of micro lens systems, such as cementing of doublets or more lenses, precise centering of the lens edge is crucial. Blocking the lens temporarily on a centering arbor ensures that the centers of all optical lens surfaces coincide with the lens edge, while the arbor's axis serves as reference for both alignment and edging process. This theoretical assumption of the traditional cementing technology is not applicable for high-end production. In reality cement wedges between the bottom lens surface and the arbor's ring knife edge may occur and even expensive arbors with single-micron precision suffer from reduced quality of the ring knife edge after multiple usages and cleaning cycles. Consequently, at least the position of the bottom lens surface is undefined and the optical axis does not coincide with the arbor's reference axis! In order to overcome this basic problem in using centering arbors, we present a novel and efficient technique which can measure and align both surfaces of a lens with respect to the arbor axis with high accuracy and furthermore align additional lenses to the optical axis of the bottom lens. This is accomplished by aligning the lens without mechanical contact to the arbor. Thus the lens can be positioned in four degrees of freedom, while the centration errors of all lens surfaces are measured and considered. Additionally the arbor's reference axis is not assumed to be aligned to the rotation axis, but simultaneously measured with high precision.

Chimpanzee ankle and foot joint kinematics: Arboreal versus terrestrial locomotion.

PubMed

Holowka, Nicholas B; O'Neill, Matthew C; Thompson, Nathan E; Demes, Brigitte

2017-09-01

Many aspects of chimpanzee ankle and midfoot joint morphology are believed to reflect adaptations for arboreal locomotion. However, terrestrial travel also constitutes a significant component of chimpanzee locomotion, complicating functional interpretations of chimpanzee and fossil hominin foot morphology. Here we tested hypotheses of foot motion and, in keeping with general assumptions, we predicted that chimpanzees would use greater ankle and midfoot joint ranges of motion during travel on arboreal supports than on the ground. We used a high-speed motion capture system to measure three-dimensional kinematics of the ankle and midfoot joints in two male chimpanzees during three locomotor modes: terrestrial quadrupedalism on a flat runway, arboreal quadrupedalism on a horizontally oriented tree trunk, and climbing on a vertically oriented tree trunk. Chimpanzees used relatively high ankle joint dorsiflexion angles during all three locomotor modes, although dorsiflexion was greatest in arboreal modes. They used higher subtalar joint coronal plane ranges of motion during terrestrial and arboreal quadrupedalism than during climbing, due in part to their use of high eversion angles in the former. Finally, they used high midfoot inversion angles during arboreal locomotor modes, but used similar midfoot sagittal plane kinematics across all locomotor modes. The results indicate that chimpanzees use large ranges of motion at their various ankle and midfoot joints during both terrestrial and arboreal locomotion. Therefore, we argue that chimpanzee foot anatomy enables a versatile locomotor repertoire, and urge caution when using foot joint morphology to reconstruct arboreal behavior in fossil hominins. © 2017 Wiley Periodicals, Inc.

Clotrimazole and econazole inhibit Streptococcus mutans biofilm and virulence in vitro.

PubMed

Qiu, Wei; Ren, Biao; Dai, Huanqin; Zhang, Lixin; Zhang, Qiong; Zhou, Xuedong; Li, Yuqing

2017-01-01

The aim of this study was to determine the inhibitory effect of eight antifungal drugs on S. mutans growth, biofilm formation and virulence factors. The actions of antifungal drugs on S. mutans were determined by recovery plates and survival kinetic curves. Biofilms were observed by scanning electron microscopy and the viable cells were recovered on BHI plates, meanwhile biofilms were stained by BacLight live/dead kit to investigate the biofilm viability. Bacteria/extracellular polysaccharides staining assays were performed to determine the EPS production of S. mutans biofilms. Acidogenicity and acidurity of S. mutans were determined using pH drop and acid tolerance assays, and the expression of ldh gene was evaluated using qPCR. We found that clotrimazole (CTR) and econazole (ECO) showed antibacterial activities on S. mutans UA159 and S. mutans clinical isolates at 12.5 and 25mg/L, respectively. CTR and ECO could also inhibit S. mutans biofilm formation and reduce the viability of preformed biofilm. CTR and ECO affected the live/dead ratio and the EPS/bacteria ratio of S. mutans biofilms. CTR and ECO also inhibited the pH drop, lactate acid production, and acid tolerance. The abilities of CTR and ECO to inhibit S. mutans ldh expression were also confirmed. We found that two antifungal azoles, CTR and ECO, had the abilities to inhibit the growth and biofilm formation of S. mutans and more importantly, they could also inhibit the virulence factors of S. mutans. Copyright © 2016 Elsevier Ltd. All rights reserved.

SOX9/miR-130a/CTR1 axis modulates DDP-resistance of cervical cancer cell.

PubMed

Feng, Chenzhe; Ma, Fang; Hu, Chunhong; Ma, Jin-An; Wang, Jingjing; Zhang, Yang; Wu, Fang; Hou, Tao; Jiang, Shun; Wang, Yapeng; Feng, Yeqian

2018-01-01

Cisplatin (DDP) -based chemotherapy is a standard strategy for cervical cancer, while chemoresistance remains a huge challenge. Copper transporter protein 1 (CTR1), a copper influx transporter required for high affinity copper (probably reduced Cu I) transport into the cell, reportedly promotes a significant fraction of DDP internalization in tumor cells. In the present study, we evaluated the function of CTR1 in the cell proliferation of cervical cancer upon DDP treatment. MicroRNAs (miRNAs) have been regarded as essential regulators of cell proliferation, apoptosis, migration, as well as chemoresistance. By using online tools, we screened for candidate miRNAs potentially regulate CTR1, among which miR-130a has been proved to promote cervical cancer cell proliferation through targeting PTEN in our previous study. In the present study, we investigated the role of miR-130a in cervical cancer chemoresistance to DDP, and confirmed the binding of miR-130a to CTR1. SOX9 also reportedly act on cancer chemoresistance. In the present study, we revealed that SOX9 inversely regulated miR-130a through direct targeting the promoter of miR-130a. Consistent with previous studies, SOX9 could affect cervical cancer chemoresistance to DDP. Taken together, we demonstrated a SOX9/miR-130a/CTR1 axis which modulated the chemoresistance of cervical cancer cell to DDP, and provided promising targets for dealing with the chemoresistance of cervical cancer.

Copper/MYC/CTR1 interplay: a dangerous relationship in hepatocellular carcinoma.

PubMed

Porcu, Cristiana; Antonucci, Laura; Barbaro, Barbara; Illi, Barbara; Nasi, Sergio; Martini, Maurizio; Licata, Anna; Miele, Luca; Grieco, Antonio; Balsano, Clara

2018-02-06

Free serum copper correlates with tumor incidence and progression of human cancers, including hepatocellular carcinoma (HCC). Copper extracellular uptake is provided by the transporter CTR1, whose expression is regulated to avoid excessive intracellular copper entry. Inadequate copper serum concentration is involved in the pathogenesis of Non Alcoholic Fatty Liver Disease (NAFLD), which is becoming a major cause of liver damage progression and HCC incidence. Finally, MYC is over-expressed in most of HCCs and is a critical regulator of cellular growth, tumor invasion and metastasis. The purpose of our study was to understand if higher serum copper concentrations might be involved in the progression of NAFLD-cirrhosis toward-HCC. We investigated whether high exogenous copper levels sensitize liver cells to transformation and if it exists an interplay between copper-related proteins and MYC oncogene. NAFLD-cirrhotic patients were characterized by a statistical significant enhancement of serum copper levels, even more evident in HCC patients. We demonstrated that high extracellular copper concentrations increase cell growth, migration, and invasion of liver cancer cells by modulating MYC/CTR1 axis. We highlighted that MYC binds a specific region of the CTR1 promoter, regulating its transcription. Accordingly, CTR1 and MYC proteins expression were progressively up-regulated in liver tissues from NAFLD-cirrhotic to HCC patients. This work provides novel insights on the molecular mechanisms by which copper may favor the progression from cirrhosis to cancer. The Cu/MYC/CTR1 interplay opens a window to refine HCC diagnosis and design new combined therapies.

Calcitonin and calcitonin receptor-like receptors: common themes with family B GPCRs?

PubMed

Barwell, James; Gingell, Joseph J; Watkins, Harriet A; Archbold, Julia K; Poyner, David R; Hay, Debbie L

2012-05-01

The calcitonin receptor (CTR) and calcitonin receptor-like receptor (CLR) are two of the 15 human family B (or Secretin-like) GPCRs. CTR and CLR are of considerable biological interest as their pharmacology is moulded by interactions with receptor activity-modifying proteins. They also have therapeutic relevance for many conditions, such as osteoporosis, diabetes, obesity, lymphatic insufficiency, migraine and cardiovascular disease. In light of recent advances in understanding ligand docking and receptor activation in both the family as a whole and in CLR and CTR specifically, this review reflects how applicable general family B GPCR themes are to these two idiosyncratic receptors. We review the main functional domains of the receptors; the N-terminal extracellular domain, the juxtamembrane domain and ligand interface, the transmembrane domain and the intracellular C-terminal domain. Structural and functional findings from the CLR and CTR along with other family B GPCRs are critically appraised to gain insight into how these domains may function. The ability for CTR and CLR to interact with receptor activity-modifying proteins adds another level of sophistication to these receptor systems but means careful consideration is needed when trying to apply generic GPCR principles. This review encapsulates current thinking in the realm of family B GPCR research by highlighting both conflicting and recurring themes and how such findings relate to two unusual but important receptors, CTR and CLR. © 2011 The Authors. British Journal of Pharmacology © 2011 The British Pharmacological Society.

Preliminary Investigation of Civil Tiltrotor in NextGen Airspace

NASA Technical Reports Server (NTRS)

Young, Larry A.; Salvano, Dan; Wright, Ken; Chung, William; Young, Ray; Miller, David; Paris, Alfanso; Gao, Huina; Cheng, Victor

2010-01-01

Presentation intro: Tiltrotor aircraft have long been envisioned as being a potentially viable means of commercial aviation transport. Preliminary results from an ongoing study into the operational and technological considerations of Civil Tiltrotor (CTR) operation in the Next Generation airspace, circa the 2025 time-frame, are presented and discussed. In particular, a fleet of CTR aircraft has been conceptually designed. The performance characteristics of this CTR fleet was subsequently translated into BADA (Base of Aircraft DAta) models that could be used as input to emulate CTR aircraft operations in the ACES and AvTerminal airspace and terminal area simulation tools. A network of nine North-Eastern corridor airports is the focus of the airspace simulation effort; the results from this airport network viII then be extrapolated to provide insights into systemic impact of CTRs on the National Airspace System (NAS). Future work will also be detailed as to attempts to model the systemic effects of noise and emissions from this fleet of new aircraft as well as assess their leveraged impact on public service missions, in time of need, such as major regional/national disaster relief efforts. The ideal outcome of this study is a set of results whereby Next Gen airspace CONOPs can be refined to reflect potential CTR capabilities and, conversely, CTR technology development efforts can be better informed as to key performance requirement thresholds needed to be met in order to successfully introduce these aircraft into civilian aviation operation.

Water-use data-collection programs and regional data base of the Great Lakes-St. Lawrence River Basin states and provinces; a comparison of withdrawal-data programs by water-use category and by state and province

USGS Publications Warehouse

Snavely, D.S.

1986-01-01

As a result of the Great Lakes Charter (signed by Illinois, Indiana, Michigan, Minnesota, New York, Ohio, Ontario, Pennsylvania, Quebec, and Wisconsin), the Geological Survey worked with a committee of the Council of Great Lakes Governors to document the water use data collection programs in the Basin. These programs are described for public water supply; supplies for domestic, irrigation, agricultural, commercial, industrial, and mining uses; and supplies for powerplants. Frequency of collection, trigger levels, storage methods, and legislative framework are described. A regional water use data base was designed to store withdrawal, diversion, and consumptive use data on a drainage basin basis. The data base will be used to decide the advisability of proposed water diversion and consumptive use projects as part of a water management strategy. Data base formats, requirements, and methods of data transmittal from each area are described. Methods for acquiring missing data are suggested. The data base will be housed by the Great Lakes Commission, Ann Arbor, Michigan. (USGS)

A rare case of anasarca caused by infiltration of the pituitary gland by diffuse large B-cell lymphoma.

PubMed

Kumabe, Ayako; Kenzaka, Tsuneaki; Nishimura, Yoshioki; Aikawa, Masaki; Mori, Masaki; Matsumura, Masami

2015-03-25

Anasarca in patients with lymphoma is a rare symptom. We report a patient with DLBCL associated with pituitary gland infiltration that was diagnosed based on significant anasarca. A 72-year-old woman with a 10-year history of hypertension visited a local hospital presenting with anasarca and 15-kg weight gain in the past 3 months. we clinically diagnosed central hypothyroidism caused by pituitary gland infiltration of diffuse large B-cell lymphoma (DLBCL) (clinical stage IV in the Ann Arbor staging classification). The first course of chemotherapy improved anasarca remarkably and the patient's body weight returned to what it was 3 months before. We experienced a patient with remarkable anasarca caused by DLBCL infiltration of the pituitary gland. A pituitary gland lesion with central hypothyroidism should be considered as one of the differential diagnoses of edema. This case was very valuable because we could assess it by following the time course of symptoms (edema and delayed relaxation time of the Achilles tendon reflex), laboratory data, and imaging findings (swelling anterior pituitary lobe).

Entropically Stabilized Colloidal Crystals Hold Entropy in Collective Modes

NASA Astrophysics Data System (ADS)

Antonaglia, James; van Anders, Greg; Glotzer, Sharon

Ordered structures can be stabilized by entropy if the system has more ordered microstates available than disordered ones. However, ``locating'' the entropy in an ordered system is challenging because entropic ordering is necessarily a collective effort emerging from the interactions of large numbers of particles. Yet, we can characterize these crystals using simple traditional tools, because entropically stabilized crystals exhibit collective motion and effective stiffness. For a two-dimensional system of hard hexagons, we calculate the dispersion relations of both vibrational and librational collective modes. We find the librational mode is gapped, and the gap provides an emergent, macroscopic, and density-dependent length scale. We quantify the entropic contribution of each collective mode and find that below this length scale, the dominant entropic contributions are librational, and above this length scale, vibrations dominate. This length scale diverges in the high-density limit, so entropy is found predominantly in libration near dense packing. National Science Foundation Graduate Research Fellowship Program Grant No. DGE 1256260, Advanced Research Computing at the University of Michigan, Ann Arbor, and the Simons Foundation.

High energy gamma-ray astronomy; Proceedings of the International Conference, ANN Arbor, MI, Oct. 2-5, 1990

NASA Astrophysics Data System (ADS)

Matthews, James

The present volume on high energy gamma-ray astronomy discusses the composition and properties of heavy cosmic rays greater than 10 exp 12 eV, implications of the IRAS Survey for galactic gamma-ray astronomy, gamma-ray emission from young neutron stars, and high-energy diffuse gamma rays. Attention is given to observations of TeV photons at the Whipple Observatory, TeV gamma rays from millisecond pulsars, recent data from the CYGNUS experiment, and recent results from the Woomera Telescope. Topics addressed include bounds on a possible He/VHE gamma-ray line signal of Galactic dark matter, albedo gamma rays from cosmic ray interactions on the solar surface, source studies, and the CANGAROO project. Also discussed are neural nets and other methods for maximizing the sensitivity of a low-threshold VHE gamma-ray telescope, a prototype water-Cerenkov air-shower detector, detection of point sources with spark chamber gamma-ray telescopes, and real-time image parameterization in high energy gamma-ray astronomy using transputers. (For individual items see A93-25002 to A93-25039)

POROSITY OF ISOLATED CELL WALLS OF SACCHAROMYCES CEREVISIAE AND BACILLUS MEGATERIUM.

PubMed

GERHARDT, P; JUDGE, J A

1964-04-01

Gerhardt, Philipp (The University of Michigan, Ann Arbor), and Jean A. Judge. Porosity of isolated cell walls of a yeast and a bacillus. J. Bacteriol. 87:945-951. 1964.-Decagram masses of cell walls were isolated from Saccharomyces cerevisiae and Bacillus megaterium; their porosity was examined by measuring the extent of uptake with polyethylene glycols and dextrans varying in molecular weight from 62 to 2,000,000. The results indicated that both walls are heteroporous. The near equality of extrapolated water-uptake values and determined moisture contents suggested that water in the cell walls is mainly free for distribution of solutes. Polymers with molecular weights of 4,500 and above were excluded by the yeast walls, and those with molecular weights of 57,000 were excluded by the bacillus walls; from these results, maximal openings of 36 and 107 A, respectively, were calculated. Electron micrographs of shadowed, stained, and sectioned walls revealed fine structure not inconsistent with heteroporosity, but the predicted openings were not seen. Altogether, in structure and permeability behavior, the cell walls were like a random meshwork of cross-linked macromolecular strands.

Permeability of bacterial spores. IV. Water content, uptake, and distribution.

PubMed

BLACK, S H; GERHARDT, P

1962-05-01

Black, S. H. (The University of Michigan, Ann Arbor) and Philipp Gerhardt. Permeability of bacterial spores. IV. Water content, uptake, and distribution. J. Bacteriol. 83:960-967. 1962.-Dormant and germinated spores of Bacillus cereus strain terminalis were examined for water properties. Respectively, they exhibited a mean density of 1.28 and 1.11 g/ml, a water content of 64.8 and 73.0%, and a total water uptake of 66.6 and 75.6%, based on spore weight, or 86.0 and 83.9%, based on spore volume. The results confirmed a previous report that internal and external water are in virtually complete equilibrium, but refuted a prevailing hypothesis that heat resistance is attributable to a dry core. A model of spore ultrastructure that evolved from the cumulative results pictures a moist, dense, heteroporous core. A new hypothesis is formulated as an explanation for thermostability in spores and possibly in other instances; it postulates the occurrence of an insolubly gelled core with cross-linking between macromolecules through stable but reversible bonds so as to form a high-polymer matrix with entrapped free water.

Federal Great Lakes fishery research objectives, priorities, and projects

USGS Publications Warehouse

Tait, Howard D.

1973-01-01

Fishery productivity of the Great Lakes has declined drastically since settlement of the area. Premium quality fishes of the Great Lakes such as whitefish, lake trout, and walleyes have been replaced by less desired species. This change is attributed to selective overfishing, pollution, and the extreme instability of fish populations. Sea lamprey predation is still a vexing problem but progress is being made in controlling this parasite. The federal fishery research program with headquarters in Ann Arbor, Michigan, has the objective of providing baseline information, needed in resource use decisions, about the fishes of the Great Lakes. Studies of the habitat requirements of fish are high priority. The program includes fish population assessments, studies of the effects of mercury and other contaminants on fish, thermal effects studies, and general investigation of the impact of engineering projects on Great Lakes fisheries. The work is closely coordinated with state and Canadian agencies through the Great Lakes Fishery Commission. Four small research vessels and four field stations are utilized with a staff of 90 and an annual budget of about $1.5 million.

A Danish population-based analysis of 105 mantle cell lymphoma patients: incidences, clinical features, response, survival and prognostic factors.

PubMed

Andersen, N S; Jensen, M K; de Nully Brown, P; Geisler, C H

2002-02-01

This study presents the first large clinical analysis of 105 unselected mantle cell lymphoma (MCL) patients diagnosed from 1992 to 2000 in a well-defined Danish population. The annual incidences were 0.7/100000 for men and 0.2/100000 for women, with no significant change during the study period. Of 97 evaluable cases, 43% achieved a complete response (CR) after initial therapy. The median disease-free (DFS) and overall survival (OS) rates were 15 and 30 months, respectively. In multivariate analysis, splenomegaly (P=0.002), anaemia (P=0.0001) and age (P=0.002), but not the international prognostic index (IPI) and the Ann Arbor staging system, had an independent impact on survival. Moreover, in a sub-analysis of 45 younger MCL patients (<65 years), a trend towards an OS plateau of 58% was observed in cases without splenomegaly and anaemia (n=29). Thus, in contrast to previously suggested prognostic factors, these variables may prove useful for clinical decisions in a significant subset of MCL patients.

More than meets the eye: the 'pink salmon patch'.

PubMed

Pallavi, Ranjita; Popescu-Martinez, Andrea

2014-08-28

Ocular adnexal lymphomas account for 1-2% of all non-Hodgkin's lymphomas. Conjunctiva is the primary site of involvement in one-third of cases. We present a case of a 47-year-old Hispanic woman who presented with left eye itching and irritation associated with a painless pink mass. Physical examination revealed the presence of a 'pink salmon-patch' involving her left medial conjunctiva. Orbital CT showed a subcentimeter left preseptal soft tissue density. Biopsy revealed a dense subepithelial lymphoid infiltrate comprised predominantly of B cells that did not coexpress CD5 or CD43. These findings were consistent with B-cell marginal zone lymphoma. Further staging assessment did not reveal disseminated disease. She had stage 1E extranodal marginal zone lymphoma as per Ann Arbor staging system. She received external beam radiotherapy to her left eye with complete resolution of the lymphoma in 2 months and continues to remain tumour free at 8-month follow-up. She will be followed up closely for development of any local (unilateral or contralateral eye) or systemic recurrence in the long run. 2014 BMJ Publishing Group Ltd.

Measurement of intrinsic rise times for various L(Y)SO and LuAG scintillators with a general study of prompt photons to achieve 10 ps in TOF-PET.

PubMed

Gundacker, Stefan; Auffray, Etiennette; Pauwels, Kristof; Lecoq, Paul

2016-04-07

The coincidence time resolution (CTR) of scintillator based detectors commonly used in positron emission tomography is well known to be dependent on the scintillation decay time (τd) and the number of photons detected (n'), i.e. CTR proportional variant √τd/n'. However, it is still an open question to what extent the scintillation rise time (τr) and other fast or prompt photons, e.g. Cherenkov photons, at the beginning of the scintillation process influence the CTR. This paper presents measurements of the scintillation emission rate for different LSO type crystals, i.e. LSO:Ce, LYSO:Ce, LSO:Ce codoped Ca and LGSO:Ce. For the various LSO-type samples measured we find an average value of 70 ps for the scintillation rise time, although some crystals like LSO:Ce codoped Ca seem to have a much faster rise time in the order of 20 ps. Additional measurements for LuAG:Ce and LuAG:Pr show a rise time of 535 ps and 251 ps, respectively. For these crystals, prompt photons (Cherenkov) can be observed at the beginning of the scintillation event. Furthermore a significantly lower rise time value is observed when codoping with calcium. To quantitatively investigate the influence of the rise time to the time resolution we measured the CTR with the same L(Y)SO samples and compared the values to Monte Carlo simulations. Using the measured relative light yields, rise- and decay times of the scintillators we are able to quantitatively understand the measured CTRs in our simulations. Although the rise time is important to fully explain the CTR variation for the different samples tested we determined its influence on the CTR to be in the order of a few percent only. This result is surprising because, if only photonstatistics of the scintillation process is considered, the CTR would be proportional to the square root of the rise time. The unexpected small rise time influence on the CTR can be explained by the convolution of the scintillation rate with the single photon time resolution (SPTR) of the photodetector and the photon travel spread (PTS) in the crystal. The timing benefits of prompt photons at the beginning of the scintillation process (Cherenkov etc) are further studied, which leads to the conclusion that the scintillation rise time, SPTR and PTS have to be lowered simultaneously to fully profit from these fast photons in order to improve the CTR significantly.

The functional coupling of the deep abdominal and paraspinal muscles: the effects of simulated paraspinal muscle contraction on force transfer to the middle and posterior layer of the thoracolumbar fascia

PubMed Central

Vleeming, A; Schuenke, M D; Danneels, L; Willard, F H

2014-01-01

The thoracolumbar fascia (TLF) consists of aponeurotic and fascial layers that interweave the paraspinal and abdominal muscles into a complex matrix stabilizing the lumbosacral spine. To better understand low back pain, it is essential to appreciate how these muscles cooperate to influence lumbopelvic stability. This study tested the following hypotheses: (i) pressure within the TLF's paraspinal muscular compartment (PMC) alters load transfer between the TLF's posterior and middle layers (PLF and MLF); and (ii) with increased tension of the common tendon of the transversus abdominis (CTrA) and internal oblique muscles and incremental PMC pressure, fascial tension is primarily transferred to the PLF. In cadaveric axial sections, paraspinal muscles were replaced with inflatable tubes to simulate paraspinal muscle contraction. At each inflation increment, tension was created in the CTrA to simulate contraction of the deep abdominal muscles. Fluoroscopic images and load cells captured changes in the size, shape and tension of the PMC due to inflation, with and without tension to the CTrA. In the absence of PMC pressure, increasing tension on the CTrA resulted in anterior and lateral movement of the PMC. PMC inflation in the absence of tension to the CTrA resulted in a small increase in the PMC perimeter and a larger posterior displacement. Combining PMC inflation and tension to the CTrA resulted in an incremental increase in PLF tension without significantly altering tension in the MLF. Paraspinal muscle contraction leads to posterior displacement of the PLF. When expansion is combined with abdominal muscle contraction, the CTrA and internal oblique transfers tension almost exclusively to the PLF, thereby girdling the paraspinal muscles. The lateral border of the PMC is restrained from displacement to maintain integrity. Posterior movement of the PMC represents an increase of the PLF extension moment arm. Dysfunctional paraspinal muscles would reduce the posterior displacement of the PLF and increase the compliance of the lateral border. The resulting change in PMC geometry could diminish any effects of increased tension of the CTrA. This study reveals a co-dependent mechanism involving balanced tension between deep abdominal and lumbar spinal muscles, which are linked through the aponeurotic components of the TLF. This implies the existence of a point of equal tension between the paraspinal muscles and the transversus abdominis and internal oblique muscles, acting through the CTrA. PMID:25139243

The functional coupling of the deep abdominal and paraspinal muscles: the effects of simulated paraspinal muscle contraction on force transfer to the middle and posterior layer of the thoracolumbar fascia.

PubMed

Vleeming, A; Schuenke, M D; Danneels, L; Willard, F H

2014-10-01

The thoracolumbar fascia (TLF) consists of aponeurotic and fascial layers that interweave the paraspinal and abdominal muscles into a complex matrix stabilizing the lumbosacral spine. To better understand low back pain, it is essential to appreciate how these muscles cooperate to influence lumbopelvic stability. This study tested the following hypotheses: (i) pressure within the TLF's paraspinal muscular compartment (PMC) alters load transfer between the TLF's posterior and middle layers (PLF and MLF); and (ii) with increased tension of the common tendon of the transversus abdominis (CTrA) and internal oblique muscles and incremental PMC pressure, fascial tension is primarily transferred to the PLF. In cadaveric axial sections, paraspinal muscles were replaced with inflatable tubes to simulate paraspinal muscle contraction. At each inflation increment, tension was created in the CTrA to simulate contraction of the deep abdominal muscles. Fluoroscopic images and load cells captured changes in the size, shape and tension of the PMC due to inflation, with and without tension to the CTrA. In the absence of PMC pressure, increasing tension on the CTrA resulted in anterior and lateral movement of the PMC. PMC inflation in the absence of tension to the CTrA resulted in a small increase in the PMC perimeter and a larger posterior displacement. Combining PMC inflation and tension to the CTrA resulted in an incremental increase in PLF tension without significantly altering tension in the MLF. Paraspinal muscle contraction leads to posterior displacement of the PLF. When expansion is combined with abdominal muscle contraction, the CTrA and internal oblique transfers tension almost exclusively to the PLF, thereby girdling the paraspinal muscles. The lateral border of the PMC is restrained from displacement to maintain integrity. Posterior movement of the PMC represents an increase of the PLF extension moment arm. Dysfunctional paraspinal muscles would reduce the posterior displacement of the PLF and increase the compliance of the lateral border. The resulting change in PMC geometry could diminish any effects of increased tension of the CTrA. This study reveals a co-dependent mechanism involving balanced tension between deep abdominal and lumbar spinal muscles, which are linked through the aponeurotic components of the TLF. This implies the existence of a point of equal tension between the paraspinal muscles and the transversus abdominis and internal oblique muscles, acting through the CTrA. © 2014 Anatomical Society.

Measurement of intrinsic rise times for various L(Y)SO and LuAG scintillators with a general study of prompt photons to achieve 10 ps in TOF-PET

NASA Astrophysics Data System (ADS)

Gundacker, Stefan; Auffray, Etiennette; Pauwels, Kristof; Lecoq, Paul

2016-04-01

The coincidence time resolution (CTR) of scintillator based detectors commonly used in positron emission tomography is well known to be dependent on the scintillation decay time ({τd} ) and the number of photons detected ({{n}\\prime} ), i.e. CTR\\propto \\sqrt{{τd}/{{n}\\prime}} . However, it is still an open question to what extent the scintillation rise time ({τr} ) and other fast or prompt photons, e.g. Cherenkov photons, at the beginning of the scintillation process influence the CTR. This paper presents measurements of the scintillation emission rate for different LSO type crystals, i.e. LSO:Ce, LYSO:Ce, LSO:Ce codoped Ca and LGSO:Ce. For the various LSO-type samples measured we find an average value of 70 ps for the scintillation rise time, although some crystals like LSO:Ce codoped Ca seem to have a much faster rise time in the order of 20 ps. Additional measurements for LuAG:Ce and LuAG:Pr show a rise time of 535 ps and 251 ps, respectively. For these crystals, prompt photons (Cherenkov) can be observed at the beginning of the scintillation event. Furthermore a significantly lower rise time value is observed when codoping with calcium. To quantitatively investigate the influence of the rise time to the time resolution we measured the CTR with the same L(Y)SO samples and compared the values to Monte Carlo simulations. Using the measured relative light yields, rise- and decay times of the scintillators we are able to quantitatively understand the measured CTRs in our simulations. Although the rise time is important to fully explain the CTR variation for the different samples tested we determined its influence on the CTR to be in the order of a few percent only. This result is surprising because, if only photonstatistics of the scintillation process is considered, the CTR would be proportional to the square root of the rise time. The unexpected small rise time influence on the CTR can be explained by the convolution of the scintillation rate with the single photon time resolution (SPTR) of the photodetector and the photon travel spread (PTS) in the crystal. The timing benefits of prompt photons at the beginning of the scintillation process (Cherenkov etc) are further studied, which leads to the conclusion that the scintillation rise time, SPTR and PTS have to be lowered simultaneously to fully profit from these fast photons in order to improve the CTR significantly.

Gait characteristics and spatio-temporal variables of climbing in bonobos (Pan paniscus).

PubMed

Schoonaert, Kirsten; D'Août, Kristiaan; Samuel, Diana; Talloen, Willem; Nauwelaerts, Sandra; Kivell, Tracy L; Aerts, Peter

2016-11-01

Although much is known about the terrestrial locomotion of great apes, their arboreal locomotion has been studied less extensively. This study investigates arboreal locomotion in bonobos (Pan paniscus), focusing on the gait characteristics and spatio-temporal variables associated with locomotion on a pole. These features are compared across different substrate inclinations (0°, 30°, 45°, 60°, and 90°), and horizontal quadrupedal walking is compared between an arboreal and a terrestrial substrate. Our results show greater variation in footfall patterns with increasing incline, resulting in more lateral gait sequences. During climbing on arboreal inclines, smaller steps and strides but higher stride frequencies and duty factors are found compared to horizontal arboreal walking. This may facilitate better balance control and dynamic stability on the arboreal substrate. We found no gradual change in spatio-temporal variables with increasing incline; instead, the results for all inclines were clustered together. Bonobos take larger strides at lower stride frequencies and lower duty factors on a horizontal arboreal substrate than on a flat terrestrial substrate. We suggest that these changes are the result of the better grip of the grasping feet on an arboreal substrate. Speed modulation of the spatio-temporal variables is similar across substrate inclinations and between substrate types, suggesting a comparable underlying motor control. Finally, we contrast these variables of arboreal inclined climbing with those of terrestrial bipedal locomotion, and briefly discuss the results with respect to the origin of habitual bipedalism. Am. J. Primatol. 78:1165-1177, 2016. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

The Extracellular Domain of Human High Affinity Copper Transporter (hNdCTR1), Synthesized by E. coli Cells, Chelates Silver and Copper Ions In Vivo

PubMed Central

Sankova, Tatiana P.; Orlov, Iurii A.; Saveliev, Andrey N.; Kirilenko, Demid A.; Babich, Polina S.; Brunkov, Pavel N.; Puchkova, Ludmila V.

2017-01-01

There is much interest in effective copper chelators to correct copper dyshomeostasis in neurodegenerative and oncological diseases. In this study, a recombinant fusion protein for expression in Escherichia coli cells was constructed from glutathione-S-transferase (GST) and the N-terminal domain (ectodomain) of human high affinity copper transporter CTR1 (hNdCTR1), which has three metal-bound motifs. Several biological properties of the GST-hNdCTR1 fusion protein were assessed. It was demonstrated that in cells, the protein was prone to oligomerization, formed inclusion bodies and displayed no toxicity. Treatment of E. coli cells with copper and silver ions reduced cell viability in a dose- and time-dependent manner. Cells expressing GST-hNdCTR1 protein demonstrated resistance to the metal treatments. These cells accumulated silver ions and formed nanoparticles that contained AgCl and metallic silver. In this bacterial population, filamentous bacteria with a length of about 10 µm were often observed. The possibility for the fusion protein carrying extracellular metal binding motifs to integrate into the cell’s copper metabolism and its chelating properties are discussed. PMID:29099786

The Extracellular Domain of Human High Affinity Copper Transporter (hNdCTR1), Synthesized by E. coli Cells, Chelates Silver and Copper Ions In Vivo.

PubMed

Sankova, Tatiana P; Orlov, Iurii A; Saveliev, Andrey N; Kirilenko, Demid A; Babich, Polina S; Brunkov, Pavel N; Puchkova, Ludmila V

2017-11-03

There is much interest in effective copper chelators to correct copper dyshomeostasis in neurodegenerative and oncological diseases. In this study, a recombinant fusion protein for expression in Escherichia coli cells was constructed from glutathione-S-transferase (GST) and the N-terminal domain (ectodomain) of human high affinity copper transporter CTR1 (hNdCTR1), which has three metal-bound motifs. Several biological properties of the GST-hNdCTR1 fusion protein were assessed. It was demonstrated that in cells, the protein was prone to oligomerization, formed inclusion bodies and displayed no toxicity. Treatment of E. coli cells with copper and silver ions reduced cell viability in a dose- and time-dependent manner. Cells expressing GST-hNdCTR1 protein demonstrated resistance to the metal treatments. These cells accumulated silver ions and formed nanoparticles that contained AgCl and metallic silver. In this bacterial population, filamentous bacteria with a length of about 10 µm were often observed. The possibility for the fusion protein carrying extracellular metal binding motifs to integrate into the cell's copper metabolism and its chelating properties are discussed.

Use of arboreal nests of tree voles (Arborimus spp.) by amphibians.

Treesearch

Eric D. Forsman; James K. Swingle

2007-01-01

We describe occupancy of arboreal nests of tree voles (Arborintus spp.) by four amphibian species in western Oregon and northern California, including clouded salamanders (Aneides ferreus), arboreal salamanders (Aneides lugubris), Pacific tree frogs (Pseudacris regilla), and a...

Evaluation of a clinical TOF-PET detector design that achieves ⩽100 ps coincidence time resolution

NASA Astrophysics Data System (ADS)

Cates, Joshua W.; Levin, Craig S.

2018-06-01

Commercially available clinical positron emission tomography (PET) detectors employ scintillation crystals that are long (20 mm length) and narrow (4–5 mm width) optically coupled on their narrow end to a photosensor. The aspect ratio of this traditional crystal rod configuration and 511 keV photon attenuation properties yield significant variances in scintillation light collection efficiency and transit time to the photodetector, due to variations in the 511 keV photon interaction depth in the crystal. These variances contribute significant to coincidence time resolution degradation. If instead, crystals are coupled to a photosensor on their long side, near-complete light collection efficiency can be achieved, and scintillation photon transit time jitter is reduced. In this work, we compare the achievable coincidence time resolution (CTR) of LGSO:Ce(0.025 mol%) crystals 3–20 mm in length when optically coupled to silicon photomultipliers (SiPMs) on either their short end or long side face. In this ‘side readout’ configuration, a CTR of 102  ±  2 ps FWHM was measured with mm3 crystals coupled to rows of mm2 SensL-J SiPMs using leading edge time pickoff and a single timing channel. This is in contrast to a CTR of 137  ±  3 ps FWHM when the same crystals were coupled to single mm2 SiPMs on their narrow ends. We further study the statistical limit on CTR using side readout via the Cramér–Rao lower bound (CRLB), with consideration given to ongoing work to further improve photosensor technologies and exploit fast phenomena to ultimately achieve 10 ps FWHM CTR. Potential design aspects of scalable front-end signal processing readout electronics using this side readout configuration are discussed. Altogether, we demonstrate that the side readout configuration offers an immediate solution for 100 ps CTR clinical PET detectors and mitigates factors prohibiting future efforts to achieve 10 ps FWHM CTR.

Increasing arboreality with altitude: a novel biogeographic dimension

PubMed Central

Scheffers, Brett R.; Phillips, Ben L.; Laurance, William F.; Sodhi, Navjot S.; Diesmos, Arvin; Williams, Stephen E.

2013-01-01

Biodiversity is spatially organized by climatic gradients across elevation and latitude. But do other gradients exist that might drive biogeographic patterns? Here, we show that rainforest's vertical strata provide climatic gradients much steeper than those offered by elevation and latitude, and biodiversity of arboreal species is organized along this gradient. In Philippine and Singaporean rainforests, we demonstrate that rainforest frogs tend to shift up in the rainforest strata as altitude increases. Moreover, a Philippine-wide dataset of frog distributions shows that frog assemblages become increasingly arboreal at higher elevations. Thus, increased arboreality with elevation at broad biogeographic scales mirrors patterns we observed at local scales. Our proposed ‘arboreality hypothesis’ suggests that the ability to exploit arboreal habitats confers the potential for larger geographical distributions because species can shift their location in the rainforest strata to compensate for shifts in temperature associated with elevation and latitude. This novel finding may help explain patterns of species richness and abundance wherever vegetation produces a vertical microclimatic gradient. Our results further suggest that global warming will ‘flatten’ the biodiversity in rainforests by pushing arboreal species towards the cooler and wetter ground. This ‘flattening’ could potentially have serious impacts on forest functioning and species survival. PMID:24026817

Increasing arboreality with altitude: a novel biogeographic dimension.

PubMed

Scheffers, Brett R; Phillips, Ben L; Laurance, William F; Sodhi, Navjot S; Diesmos, Arvin; Williams, Stephen E

2013-11-07

Biodiversity is spatially organized by climatic gradients across elevation and latitude. But do other gradients exist that might drive biogeographic patterns? Here, we show that rainforest's vertical strata provide climatic gradients much steeper than those offered by elevation and latitude, and biodiversity of arboreal species is organized along this gradient. In Philippine and Singaporean rainforests, we demonstrate that rainforest frogs tend to shift up in the rainforest strata as altitude increases. Moreover, a Philippine-wide dataset of frog distributions shows that frog assemblages become increasingly arboreal at higher elevations. Thus, increased arboreality with elevation at broad biogeographic scales mirrors patterns we observed at local scales. Our proposed 'arboreality hypothesis' suggests that the ability to exploit arboreal habitats confers the potential for larger geographical distributions because species can shift their location in the rainforest strata to compensate for shifts in temperature associated with elevation and latitude. This novel finding may help explain patterns of species richness and abundance wherever vegetation produces a vertical microclimatic gradient. Our results further suggest that global warming will 'flatten' the biodiversity in rainforests by pushing arboreal species towards the cooler and wetter ground. This 'flattening' could potentially have serious impacts on forest functioning and species survival.

The civil tiltrotor aircraft's potential in developing economies

NASA Technical Reports Server (NTRS)

Alton, Larry R.; Lane, Theodore

1991-01-01

The civilian tiltrotor (CTR) is analyzed as a new transportation technology with the potential for changing one of the key economic factors linked to Third World economic development. It is contended that efficient, low-cost transport services are a necessary condition for the economic development of Third World countries and that the CTR's capabilities and operating costs can provide more efficient transport services than have heretofore been available to such countries. A case study of potential CTR use among the nations of the Carribean Basin region appears to offer both analytical and empirical support to these contentions. The analysis indicates that normal market mechanisms are adequate for development of air cargo services using the CTR's capabilities. It is suggested that implementation of this service may require new institutional arrangements, but overall it is concluded that tiltrotor technology could make an important contribution to the economic development of Third World countries.

Cooperative Threat Reduction: Cooperation Threat Reduction Program Liquid Propellant Disposition Project

NASA Astrophysics Data System (ADS)

2002-09-01

This audit is one in a series of audits the Deputy Secretary of Defense requested. As part of the Cooperative Threat Reduction (CTR) Program, DoD agreed to assist the Russian Federation in disposing of its liquid rocket propellant. Public Law 102-228 (section 2551 NOTE, title 22, United States Code), the Soviet Nuclear Threat Reduction Act of 1991 designates DoD as the executive agent for the CTR Program. Specific objectives of the act are to destroy chemical, nuclear, and other weapons; transport, store, disable, and safeguard weapons in connection with their destruction; and establish verifiable safeguards against proliferation of weapons of mass destruction. The Office of the Assistant Secretary of Defense (International Security Policy), under the Office of the Under Secretary of Defense for Policy, develops, coordinates, and oversees implementation of policy for the CTR Program. The CTR Directorate, Defense Threat Reduction Agency operates the program.

Overcoming platinum drug resistance with copper-lowering agents.

PubMed

Chen, Helen H W; Kuo, Macus Tien

2013-10-01

Platinum (Pt)-based antitumor agents have been the mainstay of cancer chemotherapy for the last three decades. While multiple mechanisms are responsible for treatment failure, deficiency in drug transport is an important contributor. The human high-affinity copper (Cu) transporter-1 (hCtr1) can also transport Pt-based drugs including cisplatin (cDDP) and carboplatin. Reduced hCtr1 expression frequently occurs in cDDP-resistant cell lines and in cancer in patients who failed chemotherapy with these drugs. We previously demonstrated that Cu chelation induces the expression of transcription factor Sp1 which binds the promoters of Sp1 and hCtr1, thereby, up-regulating their expression, whereas Cu overload shuts down hCtr1 and Sp1 expression by dissociating Sp1 from their promoter promoters. Thus, mammalian Cu homeostasis is transcriptionally regulated within a loop consisting of Sp1, hCtr1, and Cu in a three-way mutually regulated manner. These findings suggest that it is feasible to module cDDP transport capacity through intervention of mammalian Cu homeostasis. Indeed, we found that cDDP resistance can be overcome by Cu-lowering agents through enhanced hCtr1 expression by up-regulation of Sp1 in cultured cells. This discovery provided a mechanistic basis for the ongoing clinical study using Cu chelator to overcome cDDP resistance in ovarian cancer chemotherapy. Preliminary study using copper chelator (trientine) for enhancing the treatment efficacy of carboplatin in 5 ovarian cancer patients showed encouraging results. This short review describes the perspectives of using Cu-lowering agents in overcoming Pt resistance in cancer chemotherapy.

Copper/MYC/CTR1 interplay: a dangerous relationship in hepatocellular carcinoma

PubMed Central

Barbaro, Barbara; Illi, Barbara; Nasi, Sergio; Martini, Maurizio; Licata, Anna; Miele, Luca; Grieco, Antonio; Balsano, Clara

2018-01-01

Free serum copper correlates with tumor incidence and progression of human cancers, including hepatocellular carcinoma (HCC). Copper extracellular uptake is provided by the transporter CTR1, whose expression is regulated to avoid excessive intracellular copper entry. Inadequate copper serum concentration is involved in the pathogenesis of Non Alcoholic Fatty Liver Disease (NAFLD), which is becoming a major cause of liver damage progression and HCC incidence. Finally, MYC is over-expressed in most of HCCs and is a critical regulator of cellular growth, tumor invasion and metastasis. The purpose of our study was to understand if higher serum copper concentrations might be involved in the progression of NAFLD-cirrhosis toward-HCC. We investigated whether high exogenous copper levels sensitize liver cells to transformation and if it exists an interplay between copper-related proteins and MYC oncogene. NAFLD-cirrhotic patients were characterized by a statistical significant enhancement of serum copper levels, even more evident in HCC patients. We demonstrated that high extracellular copper concentrations increase cell growth, migration, and invasion of liver cancer cells by modulating MYC/CTR1 axis. We highlighted that MYC binds a specific region of the CTR1 promoter, regulating its transcription. Accordingly, CTR1 and MYC proteins expression were progressively up-regulated in liver tissues from NAFLD-cirrhotic to HCC patients. This work provides novel insights on the molecular mechanisms by which copper may favor the progression from cirrhosis to cancer. The Cu/MYC/CTR1 interplay opens a window to refine HCC diagnosis and design new combined therapies. PMID:29507693

The Popularity of Outcome Measures for Hip and Knee Arthroplasties.

PubMed

Lovelock, Thomas M; Broughton, Nigel S; Williams, Cylie M

2018-01-01

The optimal methods of determining outcomes following hip and knee arthroplasty remain controversial. The objectives of this study were to determine the most frequently used outcome measures in randomized controlled trials (RCT) and study protocols registered with clinical trials registries (CTR) on hip and knee arthroplasty. A systematic search strategy was undertaken to identify the outcome measures used in RCT and CTR following joint arthroplasty. Databases searched included Embase, Ovid MEDLINE (including In-Process), Cochrane Central Register of Controlled Trials, CINAHL Plus, clinicaltrials.gov, ISRCTN registry, and ANZCTR. Differences in the use of outcome measures between RCT and CTR were assessed using logistic regression. There were 291 RCT and 113 CTR on hip arthroplasty and 452 RCT and 184 CTR on knee arthroplasty that met the inclusion criteria. The most popular outcome measures were the Harris Hip Score and the Knee Society Score. Multiple outcome measures were used in greater than 50% of the included studies. The Oxford Hip Score, Oxford Knee Score, EuroQol-5D, and Knee Injury and Osteoarthritis Outcome Score (all P < .001) were used in significantly more CTR than RCT. There is a clear preference for the use of the Harris Hip Score and Knee Society Score, contrary to existing international guidelines and reviews on the topic. Both measures require clinician input, which potentially influences their validity and increases their overall administration cost. Some patient-reported outcome measures, such as the Oxford Hip and Knee Scores, EuroQol-5D, and KOOS, appear to be increasing in popularity. Copyright © 2017 Elsevier Inc. All rights reserved.

Overexpression of copper transporter CTR1 in the brain barrier of North Ronaldsay sheep: implications for the study of neurodegenerative disease.

PubMed

Haywood, S; Vaillant, C

2014-01-01

Age-related regulatory failure of the brain barrier towards the influx of redox metals such as copper and iron may be associated with the pathological changes that characterize dementias such as Alzheimer's diseases (ADs) and amyotrophic lateral sclerosis (ALS). The integrity of the brain barrier to regulate copper in the brain is maintained by the complex interplay of membrane-located transporters, of which copper transporter 1 (CTR1) exerts a defining role. North Ronaldsay (NR) sheep are a primitive breed that have adapted to a copper-deficient environment by an enhanced uptake of the metal, resulting in copper overload in the liver and brain. This study reports that CTR1 is overexpressed in both the blood-brain barrier (BBB) and the blood-cerebrospinal fluid barrier (BCB) of adult NR sheep when compared with a domesticated breed. The excess copper is stored ultimately in astrocytes as non-injurious copper-metallothionein (MT). NR sheep have apparently retained an immature regulatory setting for CTR1 in the BBB, promoting facilitated copper uptake into the brain. This putative failure of maturation of CTR1 allows insight into the regulatory control of brain copper homeostasis, whereby the BBB and BCB act in concert to sequester excess copper and protect neurons from injury. The elevated copper content of the ageing human brain may derive from a dysregulation of CTR1 at the brain barrier, with a return to the default (immature) setting and implications for neurodegenerative disease. Copyright © 2013 Elsevier Ltd. All rights reserved.

Annual Research Briefs - 2000: Center for Turbulence Research

NASA Technical Reports Server (NTRS)

2000-01-01

This report contains the 2000 annual progress reports of the postdoctoral Fellows and visiting scholars of the Center for Turbulence Research (CTR). It summarizes the research efforts undertaken under the core CTR program. Last year, CTR sponsored sixteen resident Postdoctoral Fellows, nine Research Associates, and two Senior Research Fellows, hosted seven short term visitors, and supported four doctoral students. The Research Associates are supported by the Departments of Defense and Energy. The reports in this volume are divided into five groups. The first group largely consists of the new areas of interest at CTR. It includes efficient algorithms for molecular dynamics, stability in protoplanetary disks, and experimental and numerical applications of evolutionary optimization algorithms for jet flow control. The next group of reports is in experimental, theoretical, and numerical modeling efforts in turbulent combustion. As more challenging computations are attempted, the need for additional theoretical and experimental studies in combustion has emerged. A pacing item for computation of nonpremixed combustion is the prediction of extinction and re-ignition phenomena, which is currently being addressed at CTR. The third group of reports is in the development of accurate and efficient numerical methods, which has always been an important part of CTR's work. This is the tool development part of the program which supports our high fidelity numerical simulations in such areas as turbulence in complex geometries, hypersonics, and acoustics. The final two groups of reports are concerned with LES and RANS prediction methods. There has been significant progress in wall modeling for LES of high Reynolds number turbulence and in validation of the v(exp 2) - f model for industrial applications.

The effect of post-exsanguination infusion on the composition, exudation, color and post-mortem metabolic changes in lamb.

PubMed

Farouk, M M; Price, J F

1994-01-01

Twenty-four lamb carcasses were assigned to three treatment groups: (1) control (Ctr), (2) infused with 10% (vol/wt) of a tenderizing blend (NCa), and (3) NCa plus 0·015 m CaCl(2) (WCa). Results indicated that the infused carcass solution was retained in the following order: shoulder > lion > leg. Infusion had no effect (P > 0·05) on drip and cooking losses in refrigerated samples. Samples frozen and then thawed from infused carcasses had greater thaw drip (P < 0·05) and cooking losses (P < 0·01) than control samples. The amounts of drip and cooking losses were in the order: WCa > NCa > Ctr. Frozen storage preserved the red color but lowered the lightness and yellowness of ovine muscles; the opposite effect was observed following refrigerated storage. Infused samples were lighter and yellower than control in both fresh and frozen samples (P < 0·01). WCa had less red color (P < 0·01) than NCa and Ctr at all times and storage conditions. Infusion lowered (P < 0·05) the temperature of carcasses over the first 3 h postmortem (pm) compared with Ctr. The rate of glycolysis was higher in infraspinatus (IS) than in longissimus thoracis et lumborum muscle (LTL or longissimus). In both IS and LTL, glycolysis was completed within the first 6 h postmortem in NCa, whereas in Ctr and WCa, it took 12-24 h for glycolysis to be completed. The rate of glycolysis was in the order: NCa > WCa > Ctr. Copyright © 1994. Published by Elsevier Ltd.

The effect of cold application and lavender oil inhalation in cardiac surgery patients undergoing chest tube removal

PubMed Central

Hasanzadeh, Farzaneh; Kashouk, Narges Mohammadi; Amini, Shahram; Asili, Javad; Emami, Seyed Ahmad; Vashani, Hamidreza Behnam; Sahebkar, Amirhossein

2016-01-01

Post-surgical chest tube removal (CTR) is associated with a significant pain and discomfort for patients. Current treatment strategies for reducing CTR-associated pain and anxiety are limited and partially efficacious. To determine the effects of cold application, inhalation of lavender essential oil, and their combination on pain and anxiety during CTR was investigated. This randomized controlled open-label trial was conducted with 80 patients in the cardiac surgery intensive care unit who had a chest tube for duration of at least 24 hours after coronary artery bypass grafting (CABG). Patients were randomized (n=20 in each group) to receive cold application, aromatherapy with lavender oil, cold application in combination with lavender oil inhalation, or none of the above interventions (control group). The intensity and quality of pain and anxiety were evaluated using the visual analogue scale, short form and modified-McGill pain questionnaire (SFM-MPQ) and the Spielberger situational anxiety level inventory (STAII) scale, respectively. Patients in all treatment groups had significantly lower pain intensity and anxiety compared with the control group immediately, 5, 10 and 15 min after CTR. There was no statistically significant difference in the SFM-MPQ total scores between the intervention groups. With respect to anxiety score, there was a significantly reduced anxiety level immediately after CTR in the aromatherapy and cold-aromatherapy combination groups versus the cold application group. The present results suggested the efficacy of cold application and aromatherapy with lavender oil in reducing pain and anxiety associated with post-CABG CTR. PMID:27047319

High-current, relativistic electron-beam transport in metals and the role of magnetic collimation.

PubMed

Storm, M; Solodov, A A; Myatt, J F; Meyerhofer, D D; Stoeckl, C; Mileham, C; Betti, R; Nilson, P M; Sangster, T C; Theobald, W; Guo, Chunlei

2009-06-12

High-resolution coherent transition radiation (CTR) imaging diagnoses electrons accelerated in laser-solid interactions with intensities of approximately 10;{19} W/cm;{2}. The CTR images indicate electron-beam filamentation and annular propagation. The beam temperature and half-angle divergence are inferred to be approximately 1.4 MeV and approximately 16 degrees , respectively. Three-dimensional hybrid-particle-in-cell code simulations reproduce the details of the CTR images assuming an initial half-angle divergence of approximately 56 degrees . Self-generated resistive magnetic fields are responsible for the difference between the initial and measured divergence.

Surface Relaxation in Protein Crystals

NASA Technical Reports Server (NTRS)

Boutet, S.; Robinson, I. K.; Hu, Z. W.; Thomas, B. R.; Chernov, A. A.

2002-01-01

Surface X-ray diffraction measurements were performed on (111) growth faces of crystals of the Cellular iron-storage protein horse spleen ferritin. Crystal Trunkation Rods (CTR) were measured. A fit of the measured profile of the CTR revealed a surface roughness of 48 +/- 4.5 A and a top layer spacing contraction of 3.9 +/- 1.5%. In addition to the peak from the CTR, the rocking curves of the crystals displayed unexpected extra peaks. Multiple-scattering is demonstrated to account for them. Future applications of the method could allow the exploration of hydration effects on the growth of protein crystals.

Auto-SCT improves survival in systemic light chain amyloidosis: a retrospective analysis with 14-year follow-up.

PubMed

Parmar, S; Kongtim, P; Champlin, R; Dinh, Y; Elgharably, Y; Wang, M; Bashir, Q; Shah, J J; Shah, N; Popat, U; Giralt, S A; Orlowski, R Z; Qazilbash, M H

2014-08-01

Optimal treatment approach continues to remain a challenge for systemic light chain amyloidosis (AL). So far, Auto-SCT is the only modality associated with long-term survival. However, failure to show survival benefit in randomized study raises questions regarding its efficacy. We present a comparative outcome analysis of Auto-SCT to conventional therapies (CTR) in AL patients treated over a 14-year period at our institution. Out of the 145 AL amyloidosis patients, Auto-SCT was performed in 80 patients with 1-year non-relapse mortality rate of 12.5%. Novel agents were used as part of induction therapy in 56% of transplant recipients vs 46% of CTR patients. Hematological and organ responses were seen in 74.6% and 39% in the Auto-SCT arm vs 53% and 12% in the CTR arm, respectively. The projected 5-year survival for Auto-SCT vs CTR was 63% vs 38%, respectively. Landmark analysis of patients alive at 1-year after diagnosis showed improved 5-year OS of 72% with Auto-SCT vs 65% in the CTR arm. In the multivariate analysis, age <60 years, induction therapy with novel agents, kidney only involvement and Auto-SCT were associated with improved survival. In conclusion, Auto-SCT is associated with long-term survival for patients with AL amyloidosis.

How the Wisconsin Cancer Reporting System's Data Quality Task Force Started a Cancer Information Management Education Program to Improve Certified Tumor Registrar Recruitment in Wisconsin.

PubMed

Foote, Mary

2015-01-01

The role of the cancer registrar is essential in the effort to gather essential information on most types of cancer diagnosed or treated within a health care institution or within a defined population. These data are used to inform a variety of public health decisions and provide information for cancer diagnosis, treatment, and prevention programs. Effective January 1, 2015, all abstraction of medical records for cancer cases at Commission on Cancer (CoC)-accredited facilities must be performed by cancer registrars who have achieved the Certified Tumor Registrar (CTR) credential. There is a national shortage of CTRs; the National Cancer Registrars Association (NCRA) registration directory, accessed in January 2013, listed just 70 CTRs for Wisconsin. Based on the average annual number of over 29,000 invasive, consolidated cancer cases in Wisconsin (diagnosed in 2006-2010), the average number of cases per CTR was 415, while the US average was 328 cases per CTR. Using this workload estimate, in comparison with other states, Wisconsin was burdened with the sixth highest caseload per CTR in the United States and the highest in the Midwest. Further, there were only 6 Wisconsin candidates for the NCRA CTR-certification exam in 2013.

Does the Morphology of the Forelimb Flexor Muscles Differ Between Lizards Using Different Habitats?

PubMed

Lowie, Aurélien; Herrel, Anthony; Abdala, Virginia; Manzano, Adriana S; Fabre, Anne-Claire

2018-03-01

Lizards are an interesting group to study how habitat use impacts the morphology of the forelimb because they occupy a great diversity of ecological niches. In this study, we specifically investigated whether habitat use impacts the morphology of the forelimb flexor muscles in lizards. To do so, we performed dissections and quantified the physiological cross sectional area (PCSA), the fiber length, and the mass of four flexor muscles in 21 different species of lizards. Our results show that only the PCSA of the m. flexor carpi radialis is different among lizards with different ecologies (arboreal versus non-arboreal). This difference disappeared, however, when taking phylogeny into account. Arboreal species have a higher m. flexor carpi radialis cross sectional area likely allowing them to flex the wrist more forcefully which may allow them climb and hold on to branches better. In contrast, other muscles are not different between arboreal and non-arboreal species. Further studies focusing on additional anatomical features of the lizard forelimb as well as studies documenting how lizards use the arboreal niche are needed to fully understand how an arboreal life style may constrain limb morphology in lizards. Anat Rec, 301:424-433, 2018. © 2018 Wiley Periodicals, Inc. © 2018 Wiley Periodicals, Inc.

Silvicultural rehabilitation of cutover mixedwood stands

Treesearch

Laura S. Kenefic; Mohammad Bataineh; Jeremy S. Wilson; John C. Brissette; Ralph D. Nyland

2014-01-01

We investigated rehabilitation of mixedwood stands degraded by exploitative cutting on the Penobscot Experimental Forest in Maine. Three precommercial rehabilitation treatments were applied: control (no rehabilitation), moderate rehabilitation (crop tree release [CTR]), and intensive rehabilitation (CTR, timber stand improvement [TSI], and red spruce fill planting)....

Enhanced ethylene responsiveness in the Arabidopsis eer1 mutant results from a loss-of-function mutation in the protein phosphatase 2A A regulatory subunit, RCN1.

PubMed

Larsen, Paul Brian; Cancel, Jesse Daniel

2003-06-01

Ethylene signaling in Arabidopsis begins with a family of five ethylene receptors that regulate the activity of the Raf-like kinase, CTR1. Recent work to identify novel factors required for modulating ethylene signaling resulted in the isolation of enhanced ethylene response 1 (eer1), a mutant that displays both increased sensitivity and increased amplitude of response to ethylene. Molecular cloning of eer1 reveals that its mutant phenotype results from a loss-of-function mutation in the previously characterized RCN1, one of three PP2A A regulatory subunits in Arabidopsis. Our analysis shows that neither RCN1 expression nor PP2A activity is regulated by ethylene. Instead, we found that Arabidopsis PP2A-1C, a PP2A catalytic subunit previously characterized as interacting with RCN1, associates strongly with the kinase domain of CTR1 in vitro. This likely represents a role for PP2A in modulation of CTR1 activity because an in vitro kinase assay did not reveal phosphorylation of either RCN1 or PP2A-1C by CTR1, indicating that neither of them is a substrate for CTR1. PP2A activity is required for Ras-dependent activation of mammalian Raf, with reductions in PP2A activity significantly compromising the effectiveness of this mechanism. Our genetic and biochemical results suggest that a similar requirement for PP2A activity exists for ethylene signaling, with loss-of-function mutations affecting PP2A activity possibly reducing the effectiveness of CTR1 activation, thus lowering the threshold required for manifestation of ethylene response.

Novel evolutionary lineages of the invertebrate oxytocin/vasopressin superfamily peptides and their receptors in the common octopus (Octopus vulgaris)

PubMed Central

Kanda, Atsuhiro; Satake, Honoo; Kawada, Tsuyoshi; Minakata, Hiroyuki

2004-01-01

The common octopus, Octopus vulgaris, is the first invertebrate species that was shown to possess two oxytocin/vasopressin (OT/VP) superfamily peptides, octopressin (OP) and cephalotocin (CT). Previously, we cloned a GPCR (G-protein-coupled receptor) specific to CT [CTR1 (CT receptor 1)]. In the present study, we have identified an additional CTR, CTR2, and a novel OP receptor, OPR. Both CTR2 and OPR include domains and motifs typical of GPCRs, and the intron– exon structures are in accord with those of OT/VP receptor genes. CTR2 and OPR expressed in Xenopus oocytes induced calcium-mediated inward chloride current in a CT- and OP-specific manner respectively. Several regions and residues, which are requisite for binding of the vertebrate OT/VP receptor family with their ligands, are highly conserved in CTRs, but not in OPR. These different sequences between CTRs and OPR, as well as the amino acid residues of OP and CT at positions 2–5, were presumed to play crucial roles in the binding selectivity to their receptors, whereas the difference in the polarity of OT/VP family peptide residues at position 8 confers OT and VP with the binding specificity in vertebrates. CTR2 mRNA was present in various peripheral tissues, and OPR mRNA was detected in both the nervous system and peripheral tissues. Our findings suggest that the CT and OP genes, similar to the OT/VP family, evolved through duplication, but the ligand–receptor selectivity were established through different evolutionary lineages from those of their vertebrate counterparts. PMID:15504101

Patterns of livestock depredation by tiger (Panthera tigris) and leopard (Panthera pardus) in and around Corbett Tiger Reserve, Uttarakhand, India.

PubMed

Bargali, Harendra Singh; Ahmed, Tanveer

2018-01-01

India with estimated more than 2000 tigers (across 18 states) accounts for more than half of the remaining tigers across its range countries. Long-term conservation requires measures to protect the large carnivores and its prey base beyond the Protect Areas. The Corbett Tiger Reserve (CTR) and adjoining forest divisions with high density of tigers play a crucial role in conservation of tiger in Uttarakhand state as well as the Terai-Arc Landscape. However, CTR is surrounded with multiple-use forest (forest divisions), agriculture land, human habitation, townships and developmental projects. The movement of large carnivores and other wildlife through such habitats adds to the chances of human-wildlife conflict. The aim of the current study was to understand the patterns of livestock depredation by tigers and leopards in and around CTR. We examined a total of 8365 incidents of livestock depredation between 2006 and 2015 with tigers killing more livestock in a year (573.3±41.2) than leopards (263.2±9.9). Geographically, in north zone of CTR leopards were the major livestock predator (166.6±11), whereas tigers (547.7±40.1) in south zone. Examination of livestock kills indicated cows (75%) as the main victim, followed by buffaloes and other species. Analysis revealed that the livestock depredation by tigers varied significantly among seasons in south zone but not in north zone. However, such an explicit seasonal variation was not observed for leopards in north and south zone of CTR. Hotspots of livestock predation were identified around CTR. Addressing a conflict situation in a time-bound manner, timely disbursement of ex-gratia payment, involving locals at various tourism related activities and consistent rapport building initiatives are required to mitigate the human-wildlife conflict.

Effect of number of pig embryos in the uterus on their survival and development and on maternal metabolism.

PubMed

Père, M C; Dourmad, J Y; Etienne, M

1997-05-01

The effects of pig embryo number on fetal survival and growth and maternal metabolism were evaluated with 114 Large White gilts. Gilts were assigned at 38 kg to three treatments: control (CTR), ligature of the left oviduct (LIG), or right hemi-hysteroovariectomy (HHO). Insemination occurred at 311 +/- 18 d of age. A laparotomy was performed at d 35 of gestation, and gilts were slaughtered at d 112. Ovulation rate per uterine horn was 4.30, 8.70, and 17.12 in the LIG, CTR, and HHO groups, respectively. The hierarchy was the same for litter size at d 35 of gestation, but the relative differences were reduced (3.24, 5.98, and 8.40 fetuses/uterine horn, respectively). Litter size per uterine horn was similar in the CTR and HHO groups at d 112 of pregnancy (2.93, 4.69, and 4.76 fetuses in the LIG, CTR, and HHO groups, respectively). Early (before d 35 of gestation), late, and total fetal mortality increased with embryo potential per uterine horn. There was a compensation between early and late fetal mortality in the CTR and HHO groups. Fetal weight at d 112 was related to litter size in early pregnancy (1.50, 1.38, and 1.27 kg in the LIG, CTR, and HHO groups, respectively). Uterine capacity limits litter size and fetal development, even in sows with a conventional potential of embryos. Availability of energetic and gluconeogenic substrates was higher at 110 than at 60 d of gestation in the three groups. Blood substrate levels suggested that lipid mobilization and glucose uptake were higher in the gilts with a larger litter weight.

Maximization of the connectivity repertoire as a statistical principle governing the shapes of dendritic arbors

PubMed Central

Wen, Quan; Stepanyants, Armen; Elston, Guy N.; Grosberg, Alexander Y.; Chklovskii, Dmitri B.

2009-01-01

The shapes of dendritic arbors are fascinating and important, yet the principles underlying these complex and diverse structures remain unclear. Here, we analyzed basal dendritic arbors of 2,171 pyramidal neurons sampled from mammalian brains and discovered 3 statistical properties: the dendritic arbor size scales with the total dendritic length, the spatial correlation of dendritic branches within an arbor has a universal functional form, and small parts of an arbor are self-similar. We proposed that these properties result from maximizing the repertoire of possible connectivity patterns between dendrites and surrounding axons while keeping the cost of dendrites low. We solved this optimization problem by drawing an analogy with maximization of the entropy for a given energy in statistical physics. The solution is consistent with the above observations and predicts scaling relations that can be tested experimentally. In addition, our theory explains why dendritic branches of pyramidal cells are distributed more sparsely than those of Purkinje cells. Our results represent a step toward a unifying view of the relationship between neuronal morphology and function. PMID:19622738

Translational Research from an Informatics Perspective

NASA Technical Reports Server (NTRS)

Bernstam, Elmer; Meric-Bernstam, Funda; Johnson-Throop, Kathy A.; Turley, James P.; Smith, Jack W.

2007-01-01

Clinical and translational research (CTR) is an essential part of a sustainable global health system. Informatics is now recognized as an important en-abler of CTR and informaticians are increasingly called upon to help CTR efforts. The US National Institutes of Health mandated biomedical informatics activity as part of its new national CTR grant initiative, the Clinical and Translational Science Award (CTSA). Traditionally, translational re-search was defined as the translation of laboratory discoveries to patient care (bench to bedside). We argue, however, that there are many other kinds of translational research. Indeed, translational re-search requires the translation of knowledge dis-covered in one domain to another domain and is therefore an information-based activity. In this panel, we will expand upon this view of translational research and present three different examples of translation to illustrate the point: 1) bench to bedside, 2) Earth to space and 3) academia to community. We will conclude with a discussion of our local translational research efforts that draw on each of the three examples.

Calcitonin and calcitonin receptors: bone and beyond

PubMed Central

Pondel, Marc

2000-01-01

Calcitonin (CT), a 32 amino acid peptide hormone produced primarily by the thyroid, and its receptor (CTR) are well known for their ability to regulate osteoclast mediated bone resorption and enhance Ca2+ excretion by the kidney. However, recent studies now suggest that CT and CTRs may play an important role in a variety of processes as wide ranging as embryonic/foetal development and sperm function/physiology. In this review article, CT and CTR gene transcription, signal transduction and function are addressed. The effects of CT on the physiology of a variety of organ systems are discussed and the relationship between polymorphisms in the CTR gene and bone mineral density (BMD)/osteoporosis is examined. Recent studies demonstrating the ability of receptor activity modifying proteins (RAMPs) to post-translationally modify the calcitonin receptor-like receptor (CRLR) are detailed and studies employing transgenic mouse technology to determine the temporal and tissue specific transcriptional activity of the CTR gene in vivo are discussed. PMID:11298188

Civil Tiltrotor Feasibility Study for the New York and Washington Terminal Areas

NASA Technical Reports Server (NTRS)

Stouffer, Virginia; Johnson, Jesse; Gribko, Joana; Yackovetsky, Robert (Technical Monitor)

2001-01-01

NASA tasked LMI to assess the potential contributions of a yet-undeveloped Civil Tiltrotor aircraft (CTR) in improving capacity in the National Airspace System in all weather conditions. The CTRs studied have assumed operating parameters beyond current CTR capabilities. LMI analyzed CTRs three ways: in fast-time terminal area modeling simulations of New York and Washington to determine delay and throughput impacts; in the Integrated Noise Model, to determine local environmental impact; and with an economic model, to determine the price viability of a CTR. The fast-time models encompassed a 250 nmi range and included traffic interactions from local airports. Both the fast-time simulation and the noise model assessed impacts from traffic levels projected for 1999, 2007, and 2017. Results: CTRs can reduce terminal area delays due to concrete congestion in all time frames. The maximum effect, the ratio of CTRs to jets and turboprop aircraft at a subject airport should be optimized. The economic model considered US traffic only and forecasted CTR sales beginning in 2010.

Effect of time-dependent cryotherapy on redox balance of quadriceps injuries.

PubMed

Silva, Marco Aurélio dos Santos; Carvalho, Taiara Ramos de; Cruz, Amanda Cristina Marques Barros da; Jesus, Lennon Rafael Guedine de; Silva Neto, Larissa Alexsandra da; Trajano, Eduardo Tavares Lima; Bezerra, Frank Silva

2016-02-01

Muscle trauma represents a high number of injuries in professional sport and recreation and may occur through several mechanisms. This study aims at analyzing time-dependent effects of cryotherapy on the redox balance in lesioned quadriceps muscles in F1 mice. Twenty male F1 mice were divided into five groups: (a) animals were not subjected to muscle lesioning or treatment (CTR); (b) quadriceps muscle was lesioned without treatment (L); (c) quadriceps muscle was lesioned and treated with cryotherapy for 5 min (LC5); (d) quadriceps muscle was lesioned and treated with cryotherapy for 20 min (LC20); and quadriceps muscle was lesioned and treated with cryotherapy for 40 min (LC40). The mice were euthanized; the quadriceps muscles were collected and subjected to analyses for levels of protein, hydroperoxides, nitrite, catalase (CAT) activity, oxidized glutathione (GSSG) and reduced glutathione (GSH). Protein levels were reduced in L (-39%; p < 0.05), LC5 (-54%; p < 0.05), LC20 (-40%; p < 0.05) and LC40 (-50%; p < 0.05) compared to CTR. There was an increase in lipid peroxidation in L (158%; p < 0.05), LC5 (300%; p < 0.01), LC20 (292%; p < 0.01) and LC40 (362%; p < 0.01) compared to CTR. We observed a significant increase in CAT activity in L (164%; p < 0.05) and LC5 (193%; p < 0.01) compared to CTR; a significant reduction in GSH in L (-60%; p < 0.05) and LC20 (-61%; p < 0.05) compared to CTR; and a significant increase in GSSG in LC5 (171%; p < 0.05) compared to CTR. In addition, GSH/GSSG was reduced in L (-89%; p < 0.01), LC5 (-95%; p < 0.01), LC20 (-59%; p < 0.05), and LC40 (-82%; p < 0.01) compared to CTR. This study showed that the cryotherapy does not improve the oxidative stress in lesioned muscles. Copyright © 2016 Elsevier Inc. All rights reserved.

Evaluation of a clinical TOF-PET detector design that achieves ⩽100 ps coincidence time resolution.

PubMed

Cates, Joshua W; Levin, Craig S

2018-06-07

Commercially available clinical positron emission tomography (PET) detectors employ scintillation crystals that are long ([Formula: see text]20 mm length) and narrow (4-5 mm width) optically coupled on their narrow end to a photosensor. The aspect ratio of this traditional crystal rod configuration and 511 keV photon attenuation properties yield significant variances in scintillation light collection efficiency and transit time to the photodetector, due to variations in the 511 keV photon interaction depth in the crystal. These variances contribute significant to coincidence time resolution degradation. If instead, crystals are coupled to a photosensor on their long side, near-complete light collection efficiency can be achieved, and scintillation photon transit time jitter is reduced. In this work, we compare the achievable coincidence time resolution (CTR) of LGSO:Ce(0.025 mol%) crystals 3-20 mm in length when optically coupled to silicon photomultipliers (SiPMs) on either their short end or long side face. In this 'side readout' configuration, a CTR of 102  ±  2 ps FWHM was measured with [Formula: see text] mm 3 crystals coupled to rows of [Formula: see text] mm 2 SensL-J SiPMs using leading edge time pickoff and a single timing channel. This is in contrast to a CTR of 137  ±  3 ps FWHM when the same crystals were coupled to single [Formula: see text] mm 2 SiPMs on their narrow ends. We further study the statistical limit on CTR using side readout via the Cramér-Rao lower bound (CRLB), with consideration given to ongoing work to further improve photosensor technologies and exploit fast phenomena to ultimately achieve 10 ps FWHM CTR. Potential design aspects of scalable front-end signal processing readout electronics using this side readout configuration are discussed. Altogether, we demonstrate that the side readout configuration offers an immediate solution for 100 ps CTR clinical PET detectors and mitigates factors prohibiting future efforts to achieve 10 ps FWHM CTR.

Going the distance for certified cancer registrars.

PubMed

Backus, Amanda; Kolender, Ellen R

2009-01-01

Cancer registry departments are using electronic technology to solve the local and national Certified Tumor Registrar (CTR) shortages. As demand for CTRs continues to increase without an accompanied increase in the supply of qualified personnel, cancer registry departments are looking for new solutions to this growing local and national trend. In order to solve this problem, some cancer registries have started using telecommunication to fill the empty positions within their departments. This is the case at Roper St. Francis Healthcare (RSFH) in Charleston, SC, where Cancer Registry Manager, Ellen Kolender, RHIA, CTR, used telecommuting to fill one full-time and one part-time CTR position.

Simplification of arboreal marsupial assemblages in response to increasing urbanization.

PubMed

Isaac, Bronwyn; White, John; Ierodiaconou, Daniel; Cooke, Raylene

2014-01-01

Arboreal marsupials play an essential role in ecosystem function including regulating insect and plant populations, facilitating pollen and seed dispersal and acting as a prey source for higher-order carnivores in Australian environments. Primarily, research has focused on their biology, ecology and response to disturbance in forested and urban environments. We used presence-only species distribution modelling to understand the relationship between occurrences of arboreal marsupials and eco-geographical variables, and to infer habitat suitability across an urban gradient. We used post-proportional analysis to determine whether increasing urbanization affected potential habitat for arboreal marsupials. The key eco-geographical variables that influenced disturbance intolerant species and those with moderate tolerance to disturbance were natural features such as tree cover and proximity to rivers and to riparian vegetation, whereas variables for disturbance tolerant species were anthropogenic-based (e.g., road density) but also included some natural characteristics such as proximity to riparian vegetation, elevation and tree cover. Arboreal marsupial diversity was subject to substantial change along the gradient, with potential habitat for disturbance-tolerant marsupials distributed across the complete gradient and potential habitat for less tolerant species being restricted to the natural portion of the gradient. This resulted in highly-urbanized environments being inhabited by a few generalist arboreal marsupial species. Increasing urbanization therefore leads to functional simplification of arboreal marsupial assemblages, thus impacting on the ecosystem services they provide.

Simplification of Arboreal Marsupial Assemblages in Response to Increasing Urbanization

PubMed Central

Isaac, Bronwyn; White, John; Ierodiaconou, Daniel; Cooke, Raylene

2014-01-01

Arboreal marsupials play an essential role in ecosystem function including regulating insect and plant populations, facilitating pollen and seed dispersal and acting as a prey source for higher-order carnivores in Australian environments. Primarily, research has focused on their biology, ecology and response to disturbance in forested and urban environments. We used presence-only species distribution modelling to understand the relationship between occurrences of arboreal marsupials and eco-geographical variables, and to infer habitat suitability across an urban gradient. We used post-proportional analysis to determine whether increasing urbanization affected potential habitat for arboreal marsupials. The key eco-geographical variables that influenced disturbance intolerant species and those with moderate tolerance to disturbance were natural features such as tree cover and proximity to rivers and to riparian vegetation, whereas variables for disturbance tolerant species were anthropogenic-based (e.g., road density) but also included some natural characteristics such as proximity to riparian vegetation, elevation and tree cover. Arboreal marsupial diversity was subject to substantial change along the gradient, with potential habitat for disturbance-tolerant marsupials distributed across the complete gradient and potential habitat for less tolerant species being restricted to the natural portion of the gradient. This resulted in highly-urbanized environments being inhabited by a few generalist arboreal marsupial species. Increasing urbanization therefore leads to functional simplification of arboreal marsupial assemblages, thus impacting on the ecosystem services they provide. PMID:24608165

78 FR 23542 - Procurement List Additions

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2013-04-19

...: Federal Acquisition Service, GSA/FSS OFC SUP CTR--Paper Products, New York, NY Coverage: A-List for the..., NY Contracting Activity: Federal Acquisition Service, GSA/FSS OFC SUP CTR--Paper Products, New York... Procurement List. SUMMARY: This action adds products and services to the Procurement List that will be...

Arboreal seed removal and insect damage in three California oaks

Treesearch

Walter D. Koenig; Johannes M. H. Knops; William J. Carmen

2002-01-01

We investigated arboreal removal and insect damage to acorns in an undisturbed oak woodland in central coastal California. Arboreal seed removal was determined for four to eight individual Quercus lobata trees over a period of 14 years by comparing visual estimates of the acorn crop with the number of acorns caught in seed traps. Insect damage was...

Antitumoral activity of indole-3-carbinol cyclic tri- and tetrameric derivatives mixture in human breast cancer cells: in vitro and in vivo studies.

PubMed

Brandi, Giorgio; Fraternale, Alessandra; Lucarini, Simone; Paiardini, Mirko; De Santi, Mauro; Cervasi, Barbara; Paoletti, Maria F; Galluzzi, Luca; Duranti, Andrea; Magnani, Mauro

2013-05-01

Indole-3-carbinol (I3C) and its oligomeric derivatives have been widely studied for their chemopreventive and chemotherapeutic properties. We have previously shown that the I3C cyclic tetrameric derivative CTet inhibits breast cancer cell proliferation in vitro and in xenotrasplanted tumor. Here we report the antitumoral activity of a mixture of tri- and tetrameric cyclic I3C derivatives (CTr/CTet) both in vitro (MCF-7 and MDA-MB-231 breast cancer cell lines) and in vivo in a tumor xenograft model. CTr/CTet mixture avoids the low solubility drawbacks of CTet, thus favouring its solubilization, and reducing purification process, time and costs. CTr/CTet mixture has been shown to inhibit breast cancer cell proliferation (IC50 = 1.3 and 1.6 μg/ml in MCF-7 and MDAMB- 231, respectively) inducing the G0/1 cell cycle phase accumulation. The main molecular events related to CTr/CTet activity are the overexpression of p21, p27 and GADD45A, nuclear translocation of FOXO3a, inhibition of Akt activity and downregulation of estrogen receptor. In vivo, the growth of xenotransplanted tumor has been inhibited and the pro-tumoral low molecular weight cyclin E downregulation has been detected. Our data indicate that CTr/CTet is a potential anticancer combination agent for both hormone-responsive and triple-negative breast tumors.

Intravaginal probiotics modulated metabolic status and improved milk production and composition of transition dairy cows.

PubMed

Deng, Q; Odhiambo, J F; Farooq, U; Lam, T; Dunn, S M; Ametaj, B N

2016-02-01

The objective of this investigation was to evaluate whether intravaginal infusion of probiotics (a lactic acid bacteria cocktail) around parturition would influence metabolic status and increase milk production of transition dairy cows. One hundred pregnant Holstein dairy cows were assigned to 1 of the 3 experimental groups receiving intravaginal infusion of probiotics or carrier (i.e., sterile skim milk) once a week at wk -2, -1, and +1 relative to calving as follows: 2 consecutive probiotics before parturition and 1 carrier dose after parturition (TRT1), 3 consecutive probiotics doses around parturition (TRT2), and 3 consecutive carrier doses around parturition (CTR). The probiotics were a lyophilized culture mixture composed of FUA3089 and FUA3138 and FUA3140 with a cell count of 10 to 10 cfu/dose. Blood was sampled from wk -2 to +3 and milk was sampled on the third day in milk (DIM) and from wk +1 to +5 on a weekly basis. Feed intake and milk production was monitored until wk +8. Results showed that the TRT2 group (366.12 ± 49.77 μmol/L) had a lower ( = 0.01) concentration of NEFA in the serum than the CTR group (550.85 ± 47.16 μmol/L). The concentrations of IgG in the milk were 32.71 ± 3.00 mg/mL in the TRT1 group, 17.47 ± 4.54 mg/mL in the TRT2 group, and 6.73 ± 3.43 mg/mL in the CTR group at 3 DIM ( < 0.01). Meanwhile, both the TRT1 and the TRT2 group had lower haptoglobin in the milk compared with the CTR group at 3 DIM ( < 0.01). The TRT1 group had greater milk protein content than the CTR group (2.99 ± 0.04 vs. 2.82 ± 0.04%; = 0.02), whereas the TRT2 group tended to have greater lactose content compared with the CTR group (4.53 ± 0.03 vs. 4.44 ± 0.03%; = 0.05). The effect of treatment interacted with parity with regards to milk production and feed efficiency. Multiparous cows in the TRT1 and TRT2 groups had greater milk production and feed efficiency than those in the CTR group ( < 0.01 and = 0.02, respectively). Among primiparous cows, those in the TRT2 group had greater milk production ( = 0.04) whereas those in the TRT1 group had lower feed intake ( < 0.01) than those in the CTR group. Both the TRT1 and the TRT2 groups had enhanced feed efficiency compared with the CTR group ( < 0.01). In conclusion, intravaginal infusion of lactic acid bacteria modulated concentrations of selected serum metabolites and milk components and increased milk efficiency of transition dairy cows.

Center for Transportation Research | The University of Tennessee, Knoxville

Science.gov Websites

Group Tennessee Vans Research CTR Fellows Projects Publications Presentations Education Rail Courses Graders July 9-13, 2018 | 9 a.m. - Noon National Transportation Research Center (NTRC) | 2360 Cherahala -4621. Celebrating CTR's 45th Year (and counting!), 1972-2017 The Center for Transportation Research

Three descending interneurons reporting deviation from course in the locust. I. Anatomy.

PubMed

Griss, C; Rowell, C H

1986-06-01

Three descending brain interneurons (DNI, DNM, DNC) are described from Locusta migratoria. All are paired, dorsally situated neurons, with soma in the protocerebrum, input dendrites in the proto- and deuterocerebrum, and a single axon running to the metathoracic ganglion and sometimes further. In DNI the soma and all cerebral arborizations lie ipsilateral to the axon. Discrete regions of arborization lie in the ipsilateral and medial ocellar tracts, the midprotocerebrum and the deuterocerebrum. In the other ganglia the axon branches only ipsilaterally, principally laterally in the flight motor neuropil but also towards the midline. DNC is similarly organized to DNI, but the cell crosses the midline in the brain. Soma, the single projection into a lateral ocellar tract, and the midprotocerebral arborization all lie contralateral to the axon. The deuterocerebral arborization is, however, ipsilateral to the axon. The pattern of projections in the remaining ganglia resembles that of DNI. The soma and all cerebral arborizations of DNM lie ipsilateral to the axon. The arborization is only weakly subdivided into protocerebral, deuterocerebral and medial ocellar tract regions. In the remaining ganglia the arborization extends bilaterally to similar areas of both left and right flight motor neuropil. A table of synonymy is given, equating the various names used for these neurons by previous authors. The morphology correlates well with the known input and output connections. They respond physiologically to deviations from the normal flight posture mediated by ocelli, eyes and wind hairs and connect to the thoracic flight apparatus.

An improved advertising CTR prediction approach based on the fuzzy deep neural network

PubMed Central

Gao, Shu; Li, Mingjiang

2018-01-01

Combining a deep neural network with fuzzy theory, this paper proposes an advertising click-through rate (CTR) prediction approach based on a fuzzy deep neural network (FDNN). In this approach, fuzzy Gaussian-Bernoulli restricted Boltzmann machine (FGBRBM) is first applied to input raw data from advertising datasets. Next, fuzzy restricted Boltzmann machine (FRBM) is used to construct the fuzzy deep belief network (FDBN) with the unsupervised method layer by layer. Finally, fuzzy logistic regression (FLR) is utilized for modeling the CTR. The experimental results show that the proposed FDNN model outperforms several baseline models in terms of both data representation capability and robustness in advertising click log datasets with noise. PMID:29727443

An improved advertising CTR prediction approach based on the fuzzy deep neural network.

PubMed

Jiang, Zilong; Gao, Shu; Li, Mingjiang

2018-01-01

Combining a deep neural network with fuzzy theory, this paper proposes an advertising click-through rate (CTR) prediction approach based on a fuzzy deep neural network (FDNN). In this approach, fuzzy Gaussian-Bernoulli restricted Boltzmann machine (FGBRBM) is first applied to input raw data from advertising datasets. Next, fuzzy restricted Boltzmann machine (FRBM) is used to construct the fuzzy deep belief network (FDBN) with the unsupervised method layer by layer. Finally, fuzzy logistic regression (FLR) is utilized for modeling the CTR. The experimental results show that the proposed FDNN model outperforms several baseline models in terms of both data representation capability and robustness in advertising click log datasets with noise.

Archeological Surveys

NASA Technical Reports Server (NTRS)

1978-01-01

NASA remote sensing technology is being employed in archeological studies of the Anasazi Indians, who lived in New Mexico one thousand years ago. Under contract with the National Park Service, NASA's Technology Applications Center at the University of New Mexico is interpreting multispectral scanner data and demonstrating how aerospace scanning techniques can uncover features of prehistoric ruins not visible in conventional aerial photographs. The Center's initial study focused on Chaco Canyon, a pre-Columbia Anasazi site in northeastern New Mexico. Chaco Canyon is a national monument and it has been well explored on the ground and by aerial photography. But the National Park Service was interested in the potential of multispectral scanning for producing evidence of prehistoric roads, field patterns and dwelling areas not discernible in aerial photographs. The multispectral scanner produces imaging data in the invisible as well as the visible portions of the spectrum. This data is converted to pictures which bring out features not visible to the naked eye or to cameras. The Technology Applications Center joined forces with Bendix Aerospace Systems Division, Ann Arbor, Michigan, which provided a scanner-equipped airplane for mapping the Chaco Canyon area. The NASA group processed the scanner images and employed computerized image enhancement techniques to bring out additional detail.

Monitoring Outpatient Care

NASA Technical Reports Server (NTRS)

2003-01-01

Each year, health care costs for managing chronically ill patients increase as the life expectancy of Americans continues to grow. To handle this situation, many hospitals, doctors practices, and home care providers are turning to disease management, a system of coordinated health care interventions and communications, to improve outpatient care. By participating in daily monitoring programs, patients with congestive heart failure, chronic obstructive pulmonary disease, diabetes, and other chronic conditions requiring significant self-care are facing fewer emergency situations and hospitalizations. Cybernet Medical, a division of Ann Arbor, Michigan-based Cybernet Systems Corporation, is using the latest communications technology to augment the ways health care professionals monitor and assess patients with chronic diseases, while at the same time simplifying the patients interaction with technology. Cybernet s newest commercial product for this purpose evolved from research funded by NASA, the National Institute of Mental Health, and the Advanced Research Projects Agency. The research focused on the physiological assessment of astronauts and soldiers, human performance evaluation, and human-computer interaction. Cybernet Medical's MedStar Disease Management Data Collection System is an affordable, widely deployable solution for improving in-home-patient chronic disease management. The system's battery-powered and portable interface device collects physiological data from off-the-shelf instruments.

A live attenuated cold-adapted influenza A H7N3 virus vaccine provides protection against homologous and heterologous H7 viruses in mice and ferrets

DOE Office of Scientific and Technical Information (OSTI.GOV)

Joseph, Tomy; MedImmune Inc., Mountain View, CA 94043; McAuliffe, Josephine

2008-08-15

The appearance of human infections caused by avian influenza A H7 subtype viruses underscores their pandemic potential and the need to develop vaccines to protect humans from viruses of this subtype. A live attenuated H7N3 virus vaccine was generated by reverse genetics using the HA and NA genes of a low pathogenicity A/chicken/BC/CN-6/04 (H7N3) virus and the six internal protein genes of the cold-adapted A/Ann Arbor/6/60 ca (H2N2) virus. The reassortant H7N3 BC 04 ca vaccine virus was temperature sensitive and showed attenuation in mice and ferrets. Intranasal immunization with one dose of the vaccine protected mice and ferrets whenmore » challenged with homologous and heterologous H7 viruses. The reassortant H7N3 BC 04 ca vaccine virus showed comparable levels of attenuation, immunogenicity and efficacy in mice and ferret models. The safety, immunogenicity, and efficacy of this vaccine in mice and ferrets support the evaluation of this vaccine in clinical trials.« less

2011 Einstein Fellows Chosen

NASA Astrophysics Data System (ADS)

2011-03-01

ASA has announced the selection of the 2011 Einstein Fellows who will conduct research related to NASA's Physics of the Cosmos program, which aims to expand our knowledge of the origin, evolution, and fate of the Universe. The Einstein Fellowship provides support to the awardees for three years, and the Fellows may pursue their research at a host university or research center of their choosing in the United States. The new Fellows will begin their programs in the fall of 2011. The new Einstein Fellows and their host institutions are listed below: * Akos Bogdan (Smithsonian Astrophysical Observatory, Cambridge, Mass.) * Samuel Gralla (University of Maryland, College Park, Md.) * Philip Hopkins (University of California at Berkeley) * Matthew Kunz (Princeton University, Princeton, N.J.) * Laura Lopez (Massachusetts Institute of Technology, Cambridge, Mass.) * Amy Reines (National Radio Astronomy Observatory, Charlottesville, Virg.) * Rubens Reis (University of Michigan, Ann Arbor) * Ken Shen (Lawrence Berkeley National Laboratory, Berkeley, Calif.) * Jennifer Siegal-Gaskins (California Institute of Technology, Pasadena) * Lorenzo Sironi (Harvard University, Cambridge, Mass.) NASA has two other astrophysics theme-based fellowship programs: the Sagan Fellowship Program, which supports research into exoplanet exploration, and the Hubble Fellowship Program, which supports research into cosmic origins. More information on the Einstein Fellowships can be found at: http://cxc.harvard.edu/fellows/

Electron beam observation opportunity

NASA Astrophysics Data System (ADS)

Raitt, John; Banks, Peter

Ionospheric researchers will have the opportunity to conduct coordinated radio, radar, and optical observations in conjunction with other space-based electron beam experiments as part of the upcoming Cooperative High-Altitude Rocket Gun Experiments (CHARGE) 3 rocket flight in November 1988 at White Sands Missile Range (WSMR, White Sands, N.Mex.). The flight will be particularly interesting because of the high power planned for the electron gun (3.5 kV at 5 A), which is based, in part, on technology developments planned for the successful Excede series of rockets.CHARGE 3 is the third in a series of U.S. flights made in collaboration with investigators from Utah State University (Logan), Stanford University (Stanford, Calif.), the Institute for Space and Astronautical Sciences (Sendai, Japan), and the University of Michigan (Ann Arbor). The general objectives for CHARGE 3 are fourfold: to study vehicle charging and diagnosecontributory effects at higher beam currents and energy,to study ELF/VLF wave generation and propagation from dc and modulated electron beams,to diagnose beam propagation remotely by studying E region ionization and optical excitation, andto investigate voltage and current distribution in an electrically tethered space vehicle system at higher beam currents.

Studies on the drug resistance profile of Enterococcus faecium distributed from poultry retailers to hospitals.

PubMed

Limayem, Alya; Donofrio, Robert Scott; Zhang, Chao; Haller, Edward; Johnson, Michael G

2015-01-01

The multidrug resistant Enterococcus faecium (MEF) strains originating from farm animals are proliferating at a substantial pace to impact downstream food chains and could reach hospitals. This study was conducted to elucidate the drug susceptibility profile of MEF strains collected from poultry products in Ann Arbor, MI area and clinical settings from Michigan State Lab and Moffitt Cancer Center (MCC) in Florida. Presumptive positive Enterococcus isolates at species level were identified by Matrix Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) analysis. The antibiotic susceptibility profile for both poultry and clinical strains was determined by the Thermo Scientific's Sensititre conform to the National Committee for Clinical Laboratory Standards (NCCLS) and validated via quantitative real-time PCR (qPCR) methods. Out of 50 poultry samples (Turkey: n = 30; Chicken: n = 20), 36 samples were positive for Enterococcus species from which 20.83% were identified as E. faecium. All the E. faecium isolates were multidrug resistant and displayed resistance to the last alternative drug, quinupristin/dalfopristin (QD) used to treat vancomycin resistant E. faecium (VRE) in hospitals. Results indicate the presence of MEF strains in food animals and clinical settings that are also resistant to QD.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhang Xinxing; Xie Conghua; Xu Yong

Purpose: To evaluate the clinical outcome of salvage treatment for patients with relapsed natural killer (NK)/T-cell lymphoma, nasal type. Methods and Materials: Forty-four patients who had achieved complete response during initial treatment and experienced histologically proven relapse were reviewed. Twenty-nine of them received salvage treatment with radiotherapy (RT) alone (n = 7), chemotherapy (CT) alone (n = 10), or both RT and CT (n = 12); the other 15 patients received best supportive care alone. Results: The estimated 5-year overall survival (OS) rate for patients with or without salvage treatment was 37.8% vs. 0 (p < 0.0001), respectively. Salvage CTmore » did not improve survival of relapsed Stage IE and IIE patients. Among relapsed Stage IIIE and IVE patients who received salvage treatment, RT developed significantly better survival when compared with that of non-RT (1-year OS, 62.5% vs. 0, p = 0.006). Relapsed Ann Arbor stage and receiving salvage treatment were found to be significant factors influencing OS at both univariate and multivariate levels. Conclusions: Salvage treatment improved survival in patients with relapsed NK/T-cell lymphoma, nasal type. Salvage RT may play an important role in salvage treatment of relapsed extranodal NK/T-cell lymphoma.« less

Effect of cannula shape on aortic wall and flow turbulence: hydrodynamic study during extracorporeal circulation in mock thoracic aorta.

PubMed

Minakawa, Masahito; Fukuda, Ikuo; Yamazaki, Junichi; Fukui, Kozo; Yanaoka, Hideki; Inamura, Takao

2007-12-01

This study was designed to analyze flow pattern, velocity, and strain on the aortic wall of a glass aortic model during extracorporeal circulation, and to elucidate the characteristics of flow pattern in four aortic cannulas. Different patterns of large vortices and helical flow were made by each cannula. The high-velocity flow (0.6 m/s) was observed in end-hole cannula, causing high strain rate tensor (0.3~0.4 without unit) on the aortic arch. In dispersion cannula, a decreased strain rate tensor (less than 0.1) was found on the outer curvature of the aortic arch. In Soft-flow cannula (3M Cardiovascular, Ann Arbor, MI, USA), further decreased flow velocity (0.2 m/s) and strain (less than 0.2) were observed. In Select 3D cannula (Medtronic, Inc., Minneapolis, MN, USA), a high strain (0.4~0.5) was observed along the inner curvature of the aortic arch. In conclusion, end-hole cannula should not be used in atherosclerotic aorta. Particular attention should be paid both for selection of cannulas and cannulation site based on this result.

Report from the Workshop on Coregonine Restoration Science

USGS Publications Warehouse

Bronte, Charles R.; Bunnell, David B.; David, Solomon R.; Gordon, Roger; Gorsky, Dimitry; Millard, Michael J.; Read, Jennifer; Stein, Roy A.; Vaccaro, Lynn

2017-08-03

SummaryGreat Lakes fishery managers have the opportunity and have expressed interest in reestablishing a native forage base in the Great Lakes consisting of various forms and species within the genus Coregonus. This report summarizes the proceedings of a workshop focused on a subset of the genus, and the term “coregonines” is used to refer to several species of deepwater ciscoes (also known as “chubs”) and the one more pelagic-oriented cisco species (Coregonus artedi, also known as “lake herring”). As the principal conservation agency for the United States Government, the Department of Interior has unique and significant authorities and capacities to support a coregonine reestablishment program in the Great Lakes. To identify and discuss key uncertainties associated with such a program and develop a coordinated approach, the U.S. Geological Survey (USGS) and the U.S. Fish and Wildlife Service (FWS), the principal Department of the Interior bureaus to address Great Lakes fishery issues, held the first of a series of workshops on coregonine science in Ann Arbor, Michigan, on October 11–13, 2016. Workshop objectives were to identify (1) perceived key uncertainties associated with coregonine restoration in the Great Lakes and (2) DOI capacities for addressing these key uncertainties.

A live attenuated cold adapted influenza A H7N3 virus vaccine provides protection against homologous and heterologous H7 viruses in mice and ferrets

PubMed Central

Joseph, Tomy; McAuliffe, Josephine; Lu, Bin; Vogel, Leatrice; Swayne, David; Jin, Hong; Kemble, George; Subbarao, Kanta

2008-01-01

The appearance of human infections caused by avian influenza A H7 subtype viruses underscore their pandemic potential and the need to develop vaccines to protect humans from viruses of this subtype. A live attenuated H7N3 virus vaccine was generated by reverse genetics using the HA and NA genes of a low pathogenicity A/chicken/BC/CN-6/04 (H7N3) virus and the six internal protein genes of the cold-adapted A/Ann Arbor/6/60 ca (H2N2) virus. The reassortant H7N3 BC 04 ca vaccine virus was temperature sensitive and showed attenuation in mice and ferrets. Intranasal immunization with one dose of the vaccine protected mice and ferrets when challenged with homologous and heterologous H7 viruses. The reassortant H7N3 BC 04 ca vaccine virus showed comparable levels of attenuation, immunogenicity and efficacy in mice and ferret models. The safety, immunogenicity, and efficacy of this vaccine in mice and ferrets support the evaluation of this vaccine in clinical trials. PMID:18585748

Using continuous in-situ measurements to adaptively trigger urban storm water samples

NASA Astrophysics Data System (ADS)

Wong, B. P.; Kerkez, B.

2015-12-01

Until cost-effective in-situ sensors are available for biological parameters, nutrients and metals, automated samplers will continue to be the primary source of reliable water quality measurements. Given limited samples bottles, however, autosamplers often obscure insights on nutrient sources and biogeochemical processes which would otherwise be captured using a continuous sampling approach. To that end, we evaluate the efficacy a novel method to measure first-flush nutrient dynamics in flashy, urban watersheds. Our approach reduces the number of samples required to capture water quality dynamics by leveraging an internet-connected sensor node, which is equipped with a suite of continuous in-situ sensors and an automated sampler. To capture both the initial baseflow as well as storm concentrations, a cloud-hosted adaptive algorithm analyzes the high-resolution sensor data along with local weather forecasts to optimize a sampling schedule. The method was tested in a highly developed urban catchment in Ann Arbor, Michigan and collected samples of nitrate, phosphorus, and suspended solids throughout several storm events. Results indicate that the watershed does not exhibit first flush dynamics, a behavior that would have been obscured when using a non-adaptive sampling approach.

75 FR 71079 - Determination on Use of Cooperative Threat Reduction Funds in Pakistan and Afghanistan Under...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-22

... DEPARTMENT OF DEFENSE Office of the Secretary Determination on Use of Cooperative Threat Reduction Funds in Pakistan and Afghanistan Under Section 1308 of the National Defense Authorization Act for... Threat Reduction (CTR) funds for the implementation of CTR programs in Pakistan and Afghanistan will...

Proton Damage Effects on Carbon Nanotube Field-Effect Transistors

DTIC Science & Technology

2014-06-19

PROTON DAMAGE EFFECTS ON CARBON NANOTUBE FIELD-EFFECT TRANSISTORS THESIS Evan R. Kemp, Ctr...United States. AFIT-ENP-T-14-J-39 PROTON DAMAGE EFFECTS ON CARBON NANOTUBE FIELD-EFFECT TRANSISTORS THESIS Presented to...PROTON DAMAGE EFFECTS ON CARBON NANOTUBE FIELD-EFFECT TRANSISTORS Evan R. Kemp, BS Ctr, USAF Approved: // Signed

Owner ID of DCP Owner/Operators

Science.gov Websites

INDIAN AFFAIRS DEPT OF INTERIOR BLM001 BOISE INTRAGNCY FIRE CTR-BUR OF LAND MANAGEMENT DEPT OF INTERIOR BLMFIR BOISE INTRAGNCY FIRE CTR-BUR OF LAND MANAGEMENT DEPT OF INTERIOR BLMWRF White River Field Office FIRE DEPT USMC DOIFWS FISH & WILDLIFE SERVICE DEPT OF INTERIOR DRIEDU DESERT RESEARCH INSTITUTE

Copper transport and regulation in Schizosaccharomyces pombe

PubMed Central

Beaudoin, Jude; Ekici, Seda; Daldal, Fevzi; Ait-Mohand, Samia; Guérin, Brigitte; Labbé, Simon

2016-01-01

The fission yeast Schizosaccharomyces pombe has been successfully used as a model to gain fundamental knowledge in understanding how eukaryotic cells acquire copper during vegetative growth. These studies have revealed the existence of a heteromeric Ctr4–Ctr5 plasma membrane complex that mediates uptake of copper within the cells. Furthermore, additional studies have led to the identification of one of the first vacuolar copper transporters, Ctr6, as well as the copper-responsive Cuf1 transcription factor. Recent investigations have extended the use of S. pombe to elucidate new roles for copper metabolism in meiotic differentiation. For example, these studies have led to the discovery of Mfc1, which turned out to be the first example of a meiosis-specific copper transporter. Whereas copper-dependent transcriptional regulation of the Ctr family members is under the control of Cuf1 during mitosis or meiosis, meiosis-specific copper transporter Mfc1 is regulated by the recently discovered transactivator Mca1. It is foreseeable that identification of novel meiotic copper-related proteins will serve as stepping stones to unravel fundamental aspects of copper homoeostasis. PMID:24256274

Copper transport and regulation in Schizosaccharomyces pombe.

PubMed

Beaudoin, Jude; Ekici, Seda; Daldal, Fevzi; Ait-Mohand, Samia; Guérin, Brigitte; Labbé, Simon

2013-12-01

The fission yeast Schizosaccharomyces pombe has been successfully used as a model to gain fundamental knowledge in understanding how eukaryotic cells acquire copper during vegetative growth. These studies have revealed the existence of a heteromeric Ctr4-Ctr5 plasma membrane complex that mediates uptake of copper within the cells. Furthermore, additional studies have led to the identification of one of the first vacuolar copper transporters, Ctr6, as well as the copper-responsive Cuf1 transcription factor. Recent investigations have extended the use of S. pombe to elucidate new roles for copper metabolism in meiotic differentiation. For example, these studies have led to the discovery of Mfc1, which turned out to be the first example of a meiosis-specific copper transporter. Whereas copper-dependent transcriptional regulation of the Ctr family members is under the control of Cuf1 during mitosis or meiosis, meiosis-specific copper transporter Mfc1 is regulated by the recently discovered transactivator Mca1. It is foreseeable that identification of novel meiotic copper-related proteins will serve as stepping stones to unravel fundamental aspects of copper homoeostasis.

CTR1 phosphorylates the central regulator EIN2 to control ethylene hormone signaling from the ER membrane to the nucleus in Arabidopsis

PubMed Central

Ju, Chuanli; Yoon, Gyeong Mee; Shemansky, Jennifer Marie; Lin, David Y.; Ying, Z. Irene; Chang, Jianhong; Garrett, Wesley M.; Kessenbrock, Mareike; Groth, Georg; Tucker, Mark L.; Cooper, Bret; Kieber, Joseph J.; Chang, Caren

2012-01-01

The gaseous phytohormone ethylene C2H4 mediates numerous aspects of growth and development. Genetic analysis has identified a number of critical elements in ethylene signaling, but how these elements interact biochemically to transduce the signal from the ethylene receptor complex at the endoplasmic reticulum (ER) membrane to transcription factors in the nucleus is unknown. To close this gap in our understanding of the ethylene signaling pathway, the challenge has been to identify the target of the CONSTITUTIVE TRIPLE RESPONSE1 (CTR1) Raf-like protein kinase, as well as the molecular events surrounding ETHYLENE-INSENSITIVE2 (EIN2), an ER membrane-localized Nramp homolog that positively regulates ethylene responses. Here we demonstrate that CTR1 interacts with and directly phosphorylates the cytosolic C-terminal domain of EIN2. Mutations that block the EIN2 phosphorylation sites result in constitutive nuclear localization of the EIN2 C terminus, concomitant with constitutive activation of ethylene responses in Arabidopsis. Our results suggest that phosphorylation of EIN2 by CTR1 prevents EIN2 from signaling in the absence of ethylene, whereas inhibition of CTR1 upon ethylene perception is a signal for cleavage and nuclear localization of the EIN2 C terminus, allowing the ethylene signal to reach the downstream transcription factors. These findings significantly advance our understanding of the mechanisms underlying ethylene signal transduction. PMID:23132950

Calculation of the cardiothoracic ratio from portable anteroposterior chest radiography.

PubMed

Chon, Sung Bin; Oh, Won Sup; Cho, Jun Hwi; Kim, Sam Soo; Lee, Seung-Joon

2011-11-01

Cardiothoracic ratio (CTR), the ratio of cardiac diameter (CD) to thoracic diameter (TD), is a useful screening method to detect cardiomegaly, but is reliable only on posteroanterior chest radiography (chest PA). We performed this cross-sectional 3-phase study to establish reliable CTR from anteroposterior chest radiography (chest AP). First, CD(Chest PA)/CD(Chest AP) ratios were determined at different radiation distances by manipulating chest computed tomography to simulate chest PA and AP. CD(Chest PA) was inferred from multiplying CD(Chest AP) by this ratio. Incorporating this CD and substituting the most recent TD(Chest PA), we calculated the 'corrected' CTR and compared it with the conventional one in patients who took both the chest radiographies. Finally, its validity was investigated among the critically ill patients who performed portable chest AP. CD(Chest PA)/CD(Chest AP) ratio was {0.00099 × (radiation distance [cm])} + 0.79 (n = 61, r = 1.00, P < 0.001). The corrected CTR was highly correlated with the conventional one (n = 34, difference: 0.00016 ± 0.029; r = 0.92, P < 0.001). It was higher in congestive than non-congestive patients (0.53 ± 0.085; n = 38 vs 0.49 ± 0.061; n = 46, P = 0.006). Its sensitivity and specificity was 61% and 54%. In summary, reliable CTR can be calculated from chest AP with an available previous chest PA. This might help physicians detect congestive cardiomegaly for patients undergoing portable chest AP.

Prospective Evaluation of Opioid Consumption Following Carpal Tunnel Release Surgery

PubMed Central

Chapman, Talia; Kim, Nayoung; Maltenfort, Mitchell; Ilyas, Asif M.

2016-01-01

Background: Postoperative pain management and opioid consumption following carpal tunnel release (CTR) surgery may be influenced by many variables. To understand factors affecting opioid consumption, a prospective study was undertaken with the hypothesis that CTR performed under local anesthesia (wide awake local anesthesia with no tourniquet [WALANT]) would result in increased opioid consumption postoperatively compared with cases performed under sedation. Methods: All patients undergoing open CTR surgery were consecutively enrolled over a 6-month period. Information collected included patient demographics, surgical technique, amount and type of narcotic prescribed, number of pills taken, and type of anesthesia. Results: 277 patients were enrolled (56% women, 44% men). On average, 21 pills were prescribed, and 4.3 pills (median = 2) were consumed. There was no difference in consumption between patients who received WALANT (78 cases) versus (198 cases) sedation (4.9 vs 3.9 pills, respectively) (P = .22). There was no difference in opioid consumption based on insurance type (P = .47) or type of narcotic (P = .85). However, more men consumed no opioids (47%) compared with women (36%) (P < .05) and older patients consumed less than younger patients (P < .05). Conclusions: Opioid consumption following CTR is more influenced by age and gender, and less influenced by anesthesia type, insurance type, or the type of opioid prescribed. Many more opioids were prescribed than needed, on an average of 5:1. Many patients, particularly older patients, do not require any opioid analgesia after CTR. PMID:28082841

Nonylphenol and Octylphenol Differently Affect Cell Redox Balance by Modulating the Nitric Oxide Signaling

PubMed Central

Magnifico, Maria Chiara; Xhani, Marla; Popov, Milica

2018-01-01

Nonylphenol (NP) and octylphenol (OP) are pervasive environmental contaminants belonging to the broader class of compounds known as alkylphenols, with potential human toxic effects. Classified as “xenoestrogens,” NP and OP are able to interfere with the cell endocrine physiology via a direct interaction with the estrogen receptors. Here, using HepG2 cells in culture, the changes of the cell redox balance and mitochondrial activity induced by OP and NP have been investigated at μM concentrations, largely below those provoking acute toxicity, as those typical of environmental contaminants. Following 24 h cell exposure to both OP and NP, ROS production appeared significantly increased (p ≤ 0.01), together with the production of higher NO oxides (p = 0.003) and peroxynitrated protein-derivatives (NP versus CTR, p = 0.003). The mitochondrial proton electrochemical potential gradient instead was decreased (p ≤ 0.05), as the oxygen consumption by complex IV, particularly following incubation with NP (NP versus CTR, p = 0.017). Consistently, the RT-PCR and Western blot analyses proved that the OP and NP can modulate to a different extent the expression of the inducible NOS (NP versus CTR, p ≤ 0.01) and the endothelial NOS (OP versus CTR, p ≤ 0.05), with a significant variation of the coupling efficiency of the latter (NP versus CTR, p ≤ 0.05), a finding that may provide a novel clue to understand the specific xenoestrogenic properties of OP and NP. PMID:29805728

Calculation of the Cardiothoracic Ratio from Portable Anteroposterior Chest Radiography

PubMed Central

Chon, Sung Bin; Oh, Won Sup; Cho, Jun Hwi; Kim, Sam Soo

2011-01-01

Cardiothoracic ratio (CTR), the ratio of cardiac diameter (CD) to thoracic diameter (TD), is a useful screening method to detect cardiomegaly, but is reliable only on posteroanterior chest radiography (chest PA). We performed this cross-sectional 3-phase study to establish reliable CTR from anteroposterior chest radiography (chest AP). First, CDChest PA/CDChest AP ratios were determined at different radiation distances by manipulating chest computed tomography to simulate chest PA and AP. CDChest PA was inferred from multiplying CDChest AP by this ratio. Incorporating this CD and substituting the most recent TDChest PA, we calculated the 'corrected' CTR and compared it with the conventional one in patients who took both the chest radiographies. Finally, its validity was investigated among the critically ill patients who performed portable chest AP. CDChest PA/CDChest AP ratio was {0.00099 × (radiation distance [cm])} + 0.79 (n = 61, r = 1.00, P < 0.001). The corrected CTR was highly correlated with the conventional one (n = 34, difference: 0.00016 ± 0.029; r = 0.92, P < 0.001). It was higher in congestive than non-congestive patients (0.53 ± 0.085; n = 38 vs 0.49 ± 0.061; n = 46, P = 0.006). Its sensitivity and specificity was 61% and 54%. In summary, reliable CTR can be calculated from chest AP with an available previous chest PA. This might help physicians detect congestive cardiomegaly for patients undergoing portable chest AP. PMID:22065900

EphrinA2 Receptor (EphA2) Is an Invasion and Intracellular Signaling Receptor for Chlamydia trachomatis

PubMed Central

Subbarayal, Prema; Karunakaran, Karthika; Winkler, Ann-Cathrin; Rother, Marion; Gonzalez, Erik; Meyer, Thomas F.; Rudel, Thomas

2015-01-01

The obligate intracellular bacterium Chlamydia trachomatis invades into host cells to replicate inside a membrane-bound vacuole called inclusion. Multiple different host proteins are recruited to the inclusion and are functionally modulated to support chlamydial development. Invaded and replicating Chlamydia induces a long-lasting activation of the PI3 kinase signaling pathway that is required for efficient replication. We identified the cell surface tyrosine kinase EphrinA2 receptor (EphA2) as a chlamydial adherence and invasion receptor that induces PI3 kinase (PI3K) activation, promoting chlamydial replication. Interfering with binding of C. trachomatis serovar L2 (Ctr) to EphA2, downregulation of EphA2 expression or inhibition of EphA2 activity significantly reduced Ctr infection. Ctr interacts with and activates EphA2 on the cell surface resulting in Ctr and receptor internalization. During chlamydial replication, EphA2 remains active accumulating around the inclusion and interacts with the p85 regulatory subunit of PI3K to support the activation of the PI3K/Akt signaling pathway that is required for normal chlamydial development. Overexpression of full length EphA2, but not the mutant form lacking the intracellular cytoplasmic domain, enhanced PI3K activation and Ctr infection. Despite the depletion of EphA2 from the cell surface, Ctr infection induces upregulation of EphA2 through the activation of the ERK pathway, which keeps the infected cell in an apoptosis-resistant state. The significance of EphA2 as an entry and intracellular signaling receptor was also observed with the urogenital C. trachomatis-serovar D. Our findings provide the first evidence for a host cell surface receptor that is exploited for invasion as well as for receptor-mediated intracellular signaling to facilitate chlamydial replication. In addition, the engagement of a cell surface receptor at the inclusion membrane is a new mechanism by which Chlamydia subverts the host cell and induces apoptosis resistance. PMID:25906164

EphrinA2 receptor (EphA2) is an invasion and intracellular signaling receptor for Chlamydia trachomatis.

PubMed

Subbarayal, Prema; Karunakaran, Karthika; Winkler, Ann-Cathrin; Rother, Marion; Gonzalez, Erik; Meyer, Thomas F; Rudel, Thomas

2015-04-01

The obligate intracellular bacterium Chlamydia trachomatis invades into host cells to replicate inside a membrane-bound vacuole called inclusion. Multiple different host proteins are recruited to the inclusion and are functionally modulated to support chlamydial development. Invaded and replicating Chlamydia induces a long-lasting activation of the PI3 kinase signaling pathway that is required for efficient replication. We identified the cell surface tyrosine kinase EphrinA2 receptor (EphA2) as a chlamydial adherence and invasion receptor that induces PI3 kinase (PI3K) activation, promoting chlamydial replication. Interfering with binding of C. trachomatis serovar L2 (Ctr) to EphA2, downregulation of EphA2 expression or inhibition of EphA2 activity significantly reduced Ctr infection. Ctr interacts with and activates EphA2 on the cell surface resulting in Ctr and receptor internalization. During chlamydial replication, EphA2 remains active accumulating around the inclusion and interacts with the p85 regulatory subunit of PI3K to support the activation of the PI3K/Akt signaling pathway that is required for normal chlamydial development. Overexpression of full length EphA2, but not the mutant form lacking the intracellular cytoplasmic domain, enhanced PI3K activation and Ctr infection. Despite the depletion of EphA2 from the cell surface, Ctr infection induces upregulation of EphA2 through the activation of the ERK pathway, which keeps the infected cell in an apoptosis-resistant state. The significance of EphA2 as an entry and intracellular signaling receptor was also observed with the urogenital C. trachomatis-serovar D. Our findings provide the first evidence for a host cell surface receptor that is exploited for invasion as well as for receptor-mediated intracellular signaling to facilitate chlamydial replication. In addition, the engagement of a cell surface receptor at the inclusion membrane is a new mechanism by which Chlamydia subverts the host cell and induces apoptosis resistance.

Interactions with Astroglia Influence the Shape of the Developing Dendritic Arbor and Restrict Dendrite Growth Independent of Promoting Synaptic Contacts

PubMed Central

Farley, Jennifer R.; Sterritt, Jeffrey R.; Crane, Andrés B.; Wallace, Christopher S.

2017-01-01

Astroglia play key roles in the development of neurons, ranging from regulating neuron survival to promoting synapse formation, yet basic questions remain about whether astrocytes might be involved in forming the dendritic arbor. Here, we used cultured hippocampal neurons as a simple in vitro model that allowed dendritic growth and geometry to be analyzed quantitatively under conditions where the extent of interactions between neurons and astrocytes varied. When astroglia were proximal to neurons, dendrites and dendritic filopodia oriented toward them, but the general presence of astroglia significantly reduced overall dendrite growth. Further, dendritic arbors in partial physical contact with astroglia developed a pronounced pattern of asymmetrical growth, because the dendrites in direct contact were significantly smaller than the portion of the arbor not in contact. Notably, thrombospondin, the astroglial factor shown previously to promote synapse formation, did not inhibit dendritic growth. Thus, while astroglia promoted the formation of presynaptic contacts onto dendrites, dendritic growth was constrained locally within a developing arbor at sites where dendrites contacted astroglia. Taken together, these observations reveal influences on spatial orientation of growth as well as influences on morphogenesis of the dendritic arbor that have not been previously identified. PMID:28081563

Changes in species diversity of arboreal spiders in Mexican coffee agroecosystems: untangling the web of local and landscape influences driving diversity

PubMed Central

Gonthier, David J.; Marín, Linda; Iverson, Aaron L.; Perfecto, Ivette

2014-01-01

Agricultural intensification is implicated as a major driver of global biodiversity loss. Local management and landscape scale factors both influence biodiversity in agricultural systems, but there are relatively few studies to date looking at how local and landscape scales influence biodiversity in tropical agroecosystems. Understanding what drives the diversity of groups of organisms such as spiders is important from a pragmatic point of view because of the important biocontrol services they offer to agriculture. Spiders in coffee are somewhat enigmatic because of their positive or lack of response to agricultural intensification. In this study, we provide the first analysis, to our knowledge, of the arboreal spiders in the shade trees of coffee plantations. In the Soconusco region of Chiapas, Mexico we sampled across 38 sites on 9 coffee plantations. Tree and canopy connectedness were found to positively influence overall arboreal spider richness and abundance. We found that different functional groups of spiders are responding to different local and landscape factors, but overall elevation was most important variable influencing arboreal spider diversity. Our study has practical management applications that suggest having shade grown coffee offers more suitable habitat for arboreal spiders due to a variety of the characteristics of the shade trees. Our results which show consistently more diverse arboreal spider communities in lower elevations are important in light of looming global climate change. As the range of suitable elevations for coffee cultivation shrinks promoting arboreal spider diversity will be important in sustaining the viability of coffee. PMID:25392751

Wiring Economy of Pyramidal Cells in the Juvenile Rat Somatosensory Cortex

PubMed Central

Bielza, Concha; Larrañaga, Pedro; DeFelipe, Javier

2016-01-01

Ever since Cajal hypothesized that the structure of neurons is designed in such a way as to save space, time and matter, numerous researchers have analyzed wiring properties at different scales of brain organization. Here we test the hypothesis that individual pyramidal cells, the most abundant type of neuron in the cerebral cortex, optimize brain connectivity in terms of wiring length. In this study, we analyze the neuronal wiring of complete basal arborizations of pyramidal neurons in layer II, III, IV, Va, Vb and VI of the hindlimb somatosensory cortical region of postnatal day 14 rats. For each cell, we search for the optimal basal arborization and compare its length with the length of the real dendritic structure. Here the optimal arborization is defined as the arborization that has the shortest total wiring length provided that all neuron bifurcations are respected and the extent of the dendritic arborizations remain unchanged. We use graph theory and evolutionary computation techniques to search for the minimal wiring arborizations. Despite morphological differences between pyramidal neurons located in different cortical layers, we found that the neuronal wiring is near-optimal in all cases (the biggest difference between the shortest synthetic wiring found for a dendritic arborization and the length of its real wiring was less than 5%). We found, however, that the real neuronal wiring was significantly closer to the best solution found in layers II, III and IV. Our studies show that the wiring economy of cortical neurons is related not to the type of neurons or their morphological complexities but to general wiring economy principles. PMID:27832100

Wiring Economy of Pyramidal Cells in the Juvenile Rat Somatosensory Cortex.

PubMed

Anton-Sanchez, Laura; Bielza, Concha; Larrañaga, Pedro; DeFelipe, Javier

2016-01-01

Ever since Cajal hypothesized that the structure of neurons is designed in such a way as to save space, time and matter, numerous researchers have analyzed wiring properties at different scales of brain organization. Here we test the hypothesis that individual pyramidal cells, the most abundant type of neuron in the cerebral cortex, optimize brain connectivity in terms of wiring length. In this study, we analyze the neuronal wiring of complete basal arborizations of pyramidal neurons in layer II, III, IV, Va, Vb and VI of the hindlimb somatosensory cortical region of postnatal day 14 rats. For each cell, we search for the optimal basal arborization and compare its length with the length of the real dendritic structure. Here the optimal arborization is defined as the arborization that has the shortest total wiring length provided that all neuron bifurcations are respected and the extent of the dendritic arborizations remain unchanged. We use graph theory and evolutionary computation techniques to search for the minimal wiring arborizations. Despite morphological differences between pyramidal neurons located in different cortical layers, we found that the neuronal wiring is near-optimal in all cases (the biggest difference between the shortest synthetic wiring found for a dendritic arborization and the length of its real wiring was less than 5%). We found, however, that the real neuronal wiring was significantly closer to the best solution found in layers II, III and IV. Our studies show that the wiring economy of cortical neurons is related not to the type of neurons or their morphological complexities but to general wiring economy principles.

2007-2008 Weapon Systems

DTIC Science & Technology

2007-01-01

240 Tactical Operations Center ( TOC ) .......................................................................242 Tactical Unmanned Aerial...4QFY06: CHS-3 contract transferred from AMCOM Acq Ctr to CECOM Acq Ctr as a result of the PD CHS move from PM TOCs /AMDCCS to PM TRCS in FY06...SALES None CONTRACTORS IRB Bays manufacturer: General Dynamics Santa Barbara Sistemas (Kaiserslautern, Germany) Logistic support: AM General (Livonia, MI

Fishtail on a line technique for capsular tension ring insertion.

PubMed

Rixen, Jordan J; Oetting, Thomas A

2014-07-01

We describe a capsular tension ring (CTR) insertion technique that is a modification of the previously described fishtail technique. A suture line is used to pull the leading eyelet out through the main incision to form the fish configuration. Similar to the fishtail technique, this insertion technique minimizes the risk for zonular damage or a capsule tear because the CTR is not dialed into the capsular bag. The advantage of the suture line is that it prevents over bending of the CTR during insertion through the main incision, which can occur using the traditional fishtail technique. Neither author has a financial or proprietary interest in any material or method mentioned. Copyright © 2014 ASCRS and ESCRS. Published by Elsevier Inc. All rights reserved.

Annual Research Briefs, 2004: Center for Turbulence Research

NASA Technical Reports Server (NTRS)

Moin, Parviz; Mansour, Nagi N.

2004-01-01

This report contains the 2004 annual progress reports of the Research Fellows and students of the Center for Turbulence Research in its eighteenth year of operation. Since its inception in 1987, the objective of the CTR has been to advance the physical understanding of turbulent flows and development of physics based predictive tools for engineering analysis and turbulence control. Turbulence is ubiquitous in nature and in engineering devices. The studies at CTR have been motivated by applications where turbulence effects are significant; these include a broad range of technical areas such as planetary boundary layers, formation of planets, solar convection, magnetohydrodynamics, environmental and eco systems, aerodynamic noise, propulsion systems and high speed transportation. Numerical simulation has been the predominant research tool at CTR which has required a critical mass of researchers in numerical analysis and computer science in addition to core disciplines such as applied mathematics, chemical kinetics and fluid mechanics. Maintaining and promoting this interdisciplinary culture has been a hallmark of CTR and has been responsible for the realization of the results of its basic research in applications. The first group of reports in this volume are directed towards development, analysis and application of novel numerical methods for ow simulations. Development of methods for large eddy simulation of complex flows has been a central theme in this group. The second group is concerned with turbulent combustion, scalar transport and multi-phase ows. The nal group is devoted to geophysical turbulence where the problem of solar convection has been a new focus of considerable attention recently at CTR.

Effects of Bacillus subtilis natto and Different Components in Culture on Rumen Fermentation and Rumen Functional Bacteria In Vitro.

PubMed

Sun, Peng; Li, Jinan; Bu, Dengpan; Nan, Xuemei; Du, Hong

2016-05-01

This study was to investigate the effects of live or autoclaved Bacillus subtilis natto, their fermented products and media on rumen fermentation and rumen functional bacteria in vitro. Rumen fluid from three multiparous lactating Holstein cows was combined and transferred into serum bottles after diluted. Fifteen serum bottles were divided into five treatments, which were designed as following: CTR (the fermentation of 0.5 g TMR and ruminal fluids from dairy cows), LBS (CTR plus a minimum of 10(11) cfu live Bacillus subtilis natto), ABS (CTR plus a minimum of 10(11) cfu autoclaved Bacillus subtilis natto), BSC (CTR plus 1 ml Bacillus subtilis natto fermentation products without bacteria), and BSM (CTR plus 1 ml liquid fermentation medium). When separated from the culture, live Bacillus subtilis natto individually increased the concentrations of ammonia-N (P < 0.01), MCP production (P < 0.01), and tended to elevate total VFA (P = 0.07), but decreased the ratio of acetate and propionate (P < 0.01). Autoclaved Bacillus subtilis natto has the similar function with the live bacteria except for the ratio of acetate and propionate. Except B. fibrisolvens, live or autoclaved Bacillus subtilis natto did not influence or decreased the 16S rRNA gene quantification of the detected bacteria. BSC and BSM altered the relative expression of certain functional bacteria in the rumen. These results indicated that it was Bacillus subtilis natto thalli that played the important role in promoting rumen fermentation when applied as a probiotic in dairy ration.

A Mutation in the Start Codon of γ-Crystallin D Leads to Nuclear Cataracts in the Dahl SS/Jr-Ctr Strain

PubMed Central

Johnson, Ashley C.; Lee, Jonathan W.; Harmon, Ashlyn C.; Morris, Zaliya; Wang, Xuexiang; Fratkin, Jonathan; Rapp, John P.; Gomez-Sanchez, Elise; Garrett, Michael R.

2013-01-01

Cataracts are a major cause of blindness. The most common forms of cataracts are age and UV related and develops mostly in the elderly, while congenital cataracts appear at birth or in early childhood. The Dahl salt-sensitive (SS/Jr) rat is an extensively used model of salt-sensitive hypertension that exhibits concomitant renal disease. In the mid 1980’s, cataracts appeared in a few animals in the Dahl S colony, presumably the result of a spontaneous mutation. The mutation was fixed and bred to establish the SS/Jr-Ctr substrain. The SS/Jr-Ctr substrain has been exclusively used by a single investigator to study the role of steroids and hypertension. Using a classical positional cloning approach, we localized the cataract gene with high-resolution to a less than 1 Mbp region on chromosome 9 using an F1 (SS/Jr-Ctr X SHR) X SHR backcross population. The 1 Mbp region contained only 13 genes, including 4 genes from the γ-crystallins (Cryg) gene family which are known to play a role in cataract formation. All of the γ-crystallins were sequenced and a novel point mutation in the start codon (ATG → GTG) of the Crygd gene was identified which led to the complete absence of CRYGD protein in the eyes of the SS/Jr-Ctr strain. In summary, the identification of the genetic cause in this novel cataract model may provide an opportunity to better understand the development of cataracts, particularly in the context of hypertension. PMID:23404175

Forensic postmortem computed tomography: volumetric measurement of the heart and liver.

PubMed

Jakobsen, Lykke Schrøder; Lundemose, Sissel; Banner, Jytte; Lynnerup, Niels; Jacobsen, Christina

2016-12-01

The purpose of this study was to investigate the utility of postmortem computed tomography (PMCT) images in estimating organ sizes and to examine the use of the cardiothoracic ratio (CTR). We included 45 individuals (19 females), who underwent a medico-legal autopsy. Using the computer software program Mimics ® , we determined in situ heart and liver volumes derived from linear measurements (width, height and depth) on a whole body PMCT-scan, and compared the volumes with ex vivo volumes derived by CT-scan of the eviscerated heart and liver. The ex vivo volumes were also compared with the organ weights. Further, we compared the CTR with the ex vivo heart volume and a heart weight-ratio (HWR). Intra- and inter-observer analyses were performed. We found no correlation between the in situ and ex vivo volumes of the heart and liver. However, a highly significant correlation was found between the ex vivo volumes and weights of the heart and liver. No correlations between CTR and the ex vivo heart volume nor with HWR was found. Concerning cardiomegaly, we found no agreement between the CTR and HWR. The intra- and inter-observer analyses showed no significant differences. Noninvasive in situ PMCT methods for organ measuring, as performed in this study, are not useful tools in forensic pathology. The best method to estimate organ volume is a CT-scan of the eviscerated organ. PMCT-determined CTR seems to be useless for ascertaining cardiomegaly, as it neither correlated with the ex vivo heart volume nor with the HWR.

Proline isomerization in the C-terminal region of HSP27.

PubMed

Alderson, T Reid; Benesch, Justin L P; Baldwin, Andrew J

2017-07-01

In mammals, small heat-shock proteins (sHSPs) typically assemble into interconverting, polydisperse oligomers. The dynamic exchange of sHSP oligomers is regulated, at least in part, by molecular interactions between the α-crystallin domain and the C-terminal region (CTR). Here we report solution-state nuclear magnetic resonance (NMR) spectroscopy investigations of the conformation and dynamics of the disordered and flexible CTR of human HSP27, a systemically expressed sHSP. We observed multiple NMR signals for residues in the vicinity of proline 194, and we determined that, while all observed forms are highly disordered, the extra resonances arise from cis-trans peptidyl-prolyl isomerization about the G193-P194 peptide bond. The cis-P194 state is populated to near 15% at physiological temperatures, and, although both cis- and trans-P194 forms of the CTR are flexible and dynamic, both states show a residual but differing tendency to adopt β-strand conformations. In NMR spectra of an isolated CTR peptide, we observed similar evidence for isomerization involving proline 182, found within the IPI/V motif. Collectively, these data indicate a potential role for cis-trans proline isomerization in regulating the oligomerization of sHSPs.

Resting metabolic connectivity in prodromal Alzheimer's disease. A European Alzheimer Disease Consortium (EADC) project.

PubMed

Morbelli, Silvia; Drzezga, Alex; Perneczky, Robert; Frisoni, Giovanni B; Caroli, Anna; van Berckel, Bart N M; Ossenkoppele, Rik; Guedj, Eric; Didic, Mira; Brugnolo, Andrea; Sambuceti, Gianmario; Pagani, Marco; Salmon, Eric; Nobili, Flavio

2012-11-01

We explored resting-state metabolic connectivity in prodromal Alzheimer's disease (pAD) patients and in healthy controls (CTR), through a voxel-wise interregional correlation analysis of 18F-fluorodeoxyglucose (FDG)-positron emission tomography (PET) by means of statistical parametric mapping. Baseline 18F-fluorodeoxyglucose-positron emission tomography of 36 patients with amnestic mild cognitive impairment who converted to Alzheimer's disease (AD) dementia after an average time of 2 years (pAD) and of 105 CTR were processed. The area of hypometabolism in pAD showed less metabolic connectivity in patients than in CTR (autocorrelation and correlation with large temporal and frontal areas, respectively). pAD patients showed limited correlation even in selected nonhypometabolic areas, including the hippocampi and the dorsolateral prefrontal cortex (DLFC). On the contrary, in CTR group correlation was highlighted between hippocampi and precuneus/posterior cingulate and frontal cortex, and between dorsolateral prefrontal cortex and caudate nuclei and parietal cortex. The reduced metabolic connections both in hypometabolic and nonhypometabolic areas in pAD patients suggest that metabolic disconnection (reflecting early diaschisis) may antedate remote hypometabolism (early sign of synaptic degeneration). Copyright © 2012 Elsevier Inc. All rights reserved.

Early Evolution of Modern Birds Structured by Global Forest Collapse at the End-Cretaceous Mass Extinction.

PubMed

Field, Daniel J; Bercovici, Antoine; Berv, Jacob S; Dunn, Regan; Fastovsky, David E; Lyson, Tyler R; Vajda, Vivi; Gauthier, Jacques A

2018-06-04

The fossil record and recent molecular phylogenies support an extraordinary early-Cenozoic radiation of crown birds (Neornithes) after the Cretaceous-Paleogene (K-Pg) mass extinction [1-3]. However, questions remain regarding the mechanisms underlying the survival of the deepest lineages within crown birds across the K-Pg boundary, particularly since this global catastrophe eliminated even the closest stem-group relatives of Neornithes [4]. Here, ancestral state reconstructions of neornithine ecology reveal a strong bias toward taxa exhibiting predominantly non-arboreal lifestyles across the K-Pg, with multiple convergent transitions toward predominantly arboreal ecologies later in the Paleocene and Eocene. By contrast, ecomorphological inferences indicate predominantly arboreal lifestyles among enantiornithines, the most diverse and widespread Mesozoic avialans [5-7]. Global paleobotanical and palynological data show that the K-Pg Chicxulub impact triggered widespread destruction of forests [8, 9]. We suggest that ecological filtering due to the temporary loss of significant plant cover across the K-Pg boundary selected against any flying dinosaurs (Avialae [10]) committed to arboreal ecologies, resulting in a predominantly non-arboreal post-extinction neornithine avifauna composed of total-clade Palaeognathae, Galloanserae, and terrestrial total-clade Neoaves that rapidly diversified into the broad range of avian ecologies familiar today. The explanation proposed here provides a unifying hypothesis for the K-Pg-associated mass extinction of arboreal stem birds, as well as for the post-K-Pg radiation of arboreal crown birds. It also provides a baseline hypothesis to be further refined pending the discovery of additional neornithine fossils from the Latest Cretaceous and earliest Paleogene. Copyright © 2018 Elsevier Ltd. All rights reserved.

Developing neurons use a putative pioneer's peripheral arbor to establish their terminal fields.

PubMed

Gan, W B; Macagno, E R

1995-05-01

Pioneer neurons are known to guide later developing neurons during the initial phases of axonal outgrowth. To determine whether they are also important in the formation of terminal fields by the follower cells, we studied the role of a putative leech pioneer neuron, the pressure-sensitive (PD) neuron, in the establishment of other neurons' peripheral arbors. The PD neuron has a major axon that exits from its segmental ganglion to grow along the dorsal-posterior (DP) nerve to the dorsal body wall, where it arborizes extensively mainly in its own segment. It also has two minor axons that project to the two adjacent segments but branch to a lesser degree. We found that the peripheral projections of several later developing neurons, including the AP motor neuron and the TD sensory neuron, followed, with great precision, the major axon and peripheral arbor of the consegmental PD neuron, up to its fourth-order branches. When a PD neuron was ablated before it had grown to the body wall, the AP and TD axons grew normally toward and reached the target area, but then formed terminal arbors that were greatly reduced in size and abnormal in morphology. Further, if the ablation of a PD neuron was accompanied by the induction, in the same segment, of greater outgrowth of the minor axon of a PD neuron from the adjacent segment, the arbors of the same AP neurons grew along these novel PD neuron branches. These results demonstrate that the peripheral arbor of a PD neuron is a both necessary and sufficient template for the formation of normal terminal fields by certain later growing follower neurons.

Population-scale three-dimensional reconstruction and quantitative profiling of microglia arbors

PubMed Central

Rey-Villamizar, Nicolas; Merouane, Amine; Lu, Yanbin; Mukherjee, Amit; Trett, Kristen; Chong, Peter; Harris, Carolyn; Shain, William; Roysam, Badrinath

2015-01-01

Motivation: The arbor morphologies of brain microglia are important indicators of cell activation. This article fills the need for accurate, robust, adaptive and scalable methods for reconstructing 3-D microglial arbors and quantitatively mapping microglia activation states over extended brain tissue regions. Results: Thick rat brain sections (100–300 µm) were multiplex immunolabeled for IBA1 and Hoechst, and imaged by step-and-image confocal microscopy with automated 3-D image mosaicing, producing seamless images of extended brain regions (e.g. 5903 × 9874 × 229 voxels). An over-complete dictionary-based model was learned for the image-specific local structure of microglial processes. The microglial arbors were reconstructed seamlessly using an automated and scalable algorithm that exploits microglia-specific constraints. This method detected 80.1 and 92.8% more centered arbor points, and 53.5 and 55.5% fewer spurious points than existing vesselness and LoG-based methods, respectively, and the traces were 13.1 and 15.5% more accurate based on the DIADEM metric. The arbor morphologies were quantified using Scorcioni’s L-measure. Coifman’s harmonic co-clustering revealed four morphologically distinct classes that concord with known microglia activation patterns. This enabled us to map spatial distributions of microglial activation and cell abundances. Availability and implementation: Experimental protocols, sample datasets, scalable open-source multi-threaded software implementation (C++, MATLAB) in the electronic supplement, and website (www.farsight-toolkit.org). http://www.farsight-toolkit.org/wiki/Population-scale_Three-dimensional_Reconstruction_and_Quanti-tative_Profiling_of_Microglia_Arbors Contact: broysam@central.uh.edu Supplementary information: Supplementary data are available at Bioinformatics online. PMID:25701570

Unsupervised segmentation of lung fields in chest radiographs using multiresolution fractal feature vector and deformable models.

PubMed

Lee, Wen-Li; Chang, Koyin; Hsieh, Kai-Sheng

2016-09-01

Segmenting lung fields in a chest radiograph is essential for automatically analyzing an image. We present an unsupervised method based on multiresolution fractal feature vector. The feature vector characterizes the lung field region effectively. A fuzzy c-means clustering algorithm is then applied to obtain a satisfactory initial contour. The final contour is obtained by deformable models. The results show the feasibility and high performance of the proposed method. Furthermore, based on the segmentation of lung fields, the cardiothoracic ratio (CTR) can be measured. The CTR is a simple index for evaluating cardiac hypertrophy. After identifying a suspicious symptom based on the estimated CTR, a physician can suggest that the patient undergoes additional extensive tests before a treatment plan is finalized.

Disruption of copper-dependent signaling pathway in the nitrofen-induced congenital diaphragmatic hernia.

PubMed

Takahashi, Toshiaki; Friedmacher, Florian; Takahashi, Hiromizu; Hofmann, Alejandro Daniel; Puri, Prem

2015-01-01

Normal development of the fetal diaphragm requires muscularization of the diaphragm as well as the structural integrity of its underlying connective tissue components. Developmental mutations that inhibit the formation of extracellular matrix (ECM) have been shown to result in congenital diaphragmatic hernia (CDH). Copper (Cu) is an important element during diaphragm morphogenesis by participating in cross-linking of collagen and elastin fibers. Cu transport is strictly regulated by two membrane proteins: Cu-uptake transporter 1 (CTR1) and the Cu-efflux pump ATP7A. Animals lacking Cu-dependent enzymes exhibit abnormal connective tissue with diaphragmatic defects. However, the molecular basis of disruptions in Cu-mediated ECM formation in CDH remains unclear. We designed this study to investigate the hypothesis that diaphragmatic expression of CTR1 and ATP7A is decreased in the nitrofen-induced CDH model. Timed-pregnant rats were exposed to either nitrofen or vehicle on gestational day 9 (D9), and fetuses were harvested on selected time-points D15 and D18. Microdissected fetal diaphragms (n = 48) were divided into control and nitrofen-induced CDH samples (n = 12 per experimental group and time-point). Diaphragmatic gene expression levels of CTR1 and ATP7A were analyzed by quantitative real-time polymerase chain reaction. Immunohistochemistry was performed to evaluate CTR1 and ATP7A protein expression in fetal diaphragms, which was combined with specific rhodanine staining to determine diaphragmatic Cu content. Relative mRNA levels of CTR1 and ATP7A were significantly reduced in diaphragms of nitrofen-exposed fetuses on D15 (0.06 ± 0.02 vs. 0.18 ± 0.08; p < 0.05 and 0.04 ± 0.02 vs. 0.08 ± 0.02; p < 0.05) and D18 (0.10 ± 0.03 vs. 0.17 ± 0.02; p < 0.05 and 0.09 ± 0.03 vs. 0.16 ± 0.04; p < 0.05) compared to controls. Immunoreactivity of CTR1 and ATP7A was markedly decreased in the malformed diaphragmatic ECM of nitrofen-exposed fetuses on D15 and D18, which was associated with a significantly decreased diaphragmatic Cu content on D15 (7.22 ± 2.91 vs. 17.50 ± 3.09; p < 0.05) and D18 (17.60 ± 3.54 vs. 28.20 ± 4.63; p < 0.05) compared to controls. Reduced diaphragmatic expression of CTR1 and ATP7A during morphogenesis may impair the activity of Cu-dependent enzymes and thus contribute to defective ECM during diaphragmatic development.

Exploring the optimal economic timing for crop tree release treatments in hardwoods: results from simulation

Treesearch

Chris B. LeDoux; Gary W. Miller

2008-01-01

In this study we used data from 16 Appalachian hardwood stands, a growth and yield computer simulation model, and stump-to-mill logging cost-estimating software to evaluate the optimal economic timing of crop tree release (CTR) treatments. The simulated CTR treatments consisted of one-time logging operations at stand age 11, 23, 31, or 36 years, with the residual...

A Descriptive Study of the Tuition Reimbursement Policies of Ten Major Corporations in Cuyahoga County, Ohio.

ERIC Educational Resources Information Center

Lidstrom, Kermit

This study was conducted to learn about the impact and extent of Corporate Tuition Reimbursement (CTR) in Cuyahoga County, Ohio. Tuition is a factor in Ohio because tuition is charged in the community colleges. Literature on the trends in income sources in higher education and the shift to older part-time students are reviewed. Ten CTR policies of…

Mutations That Affect the Efficiency of Translation of mRNA for the cII Gene of Coliphage Lambda

PubMed Central

Dul, Ed; Mahoney, Michael E.; Wulff, Daniel L.

1987-01-01

Starting with the λ pRE- strain λctr1 cy3008, which forms clear plaques, we have isolated two mutant strains, λdya2 ctr1 cy3008 and λ dya3 ctr1 cy3008, that form plaques with very slightly turbid centers. The dya2 and dya3 mutations lie in the region of overlap between the PRE promoter and the ribosome recognition region of the cII gene, and have nucleotide alterations at positions -1 and +5 of pRE, and alterations of cII mRNA at -16 and -21 nucleotides before the initial AUG codon of the gene. Both mutations destabilize a stem structure that may be formed by cII mRNA, and dya2 also changes the sequence on cII mRNA that is complementary to the 3'-end of 16 S rRNA from 5'-UAAGGA-3' to 5'-UGAGGA-3'.—The dya2 and dya3 mutations, along with the ctr1 mutation, which destabilizes either of two alternate stem structures which may be formed by cII mRNA (these being more stable stem structures than the one affected by dya2 and dya3), were tested for their ability to reverse two cII- mutations that are characterized by inefficient translation of cII mRNA. These are cII3088, an A → G mutation four bases before the initial AUG codon, and cII3059 , a GUU → GAU (Val2 → Asp) second codon mutation. It was found that ctr1 completely reverses the translation defects of these two mutations, while dya2 partially reverses these translation defects. The dya3 mutation has no effect on translation efficiency under any condition tested. However neither the ctr1 mutation nor the dya2 mutation has much effect on translation efficiency in an otherwise cII+ background, indicating that other factors must limit the rate of translation of cII mRNA under these conditions. PMID:2953647

Comparisons of bone mineral density and bone quality in adult rock climbers, resistance-trained men, and untrained men.

PubMed

Sherk, Vanessa D; Bemben, Michael G; Bemben, Debra A

2010-09-01

The nature of muscular contractions and episodes of impact loading during technical rock climbing are often varied and complex, and the resulting effects on bone health are unclear. The purpose of this study was to compare total body, lumbar spine, proximal femur, and forearm areal bone mineral density (aBMD) and tibia and forearm bone quality in male rock climbers (RC) (n = 15), resistance trained men (RT) (n = 16), and untrained male controls (CTR) (n = 16). Total body, anteroposterior (AP) lumbar spine, proximal femur, and forearm aBMD and body composition were measured using dual-energy X-ray absorptiometry (DXA) (Lunar Prodigy, v. 10.50.086; GE Healthcare, Waukesha, Wisconsin, U.S.A.). Volumetric BMD (vBMD), bone content, bone area, and muscle cross-sectional area (MCSA) of the tibia and forearm were measured using pQCT (peripheral quantitative computed tomography; Stratec XCT 3000, Pforzheim, Germany). No significant group differences were seen in bone-free lean body mass. CTR had significantly (p < 0.05) greater body fat % than RC and RT and significantly (p < 0.05) greater fat mass than RC. Lumbar spine and femoral neck aBMD were significantly (p < 0.05) greater in RT compared to both RC and CTR. RC had significantly (p < 0.05) lower aBMD at the 33% radius site than CTR. Forearm MCSA was significantly (p < 0.05) lower in CTR than in the other groups. No significant differences were seen between groups for vBMD or bone area of the tibia and forearm. In conclusion, resistance-trained men had higher bone density at the central skeletal sites than rock climbers; however, bone quality variables of the peripheral limbs were similar in rock climber and resistance-trained groups.

Primary treatment regimen and diabetes insipidus as predictors of health outcomes in adults with childhood-onset craniopharyngioma.

PubMed

Yuen, Kevin C J; Kołtowska-Häggström, Maria; Cook, David M; Fox, Janet L; Jönsson, Peter J; Geffner, Mitchell E; Abs, Roger

2014-04-01

Craniopharyngiomas are often associated with significant morbidity due to their location and treatment effects. Little is known of the effects of primary treatment regimen and diabetes insipidus (DI), a clinical surrogate of hypothalamic obesity, on health outcomes in adults with childhood-onset craniopharyngioma (COCP). The objective of the study was to examine health outcomes of adults with COCP based on primary treatment regimens and the presence of DI. This study included a retrospective KIMS (Pfizer International Metabolic Database) data analysis of 180 adults with COCP according to the primary treatment regimen [one surgery (1Surg) vs complex treatment regimen (CTrR) of more than 1Surg and/or radiotherapy] and the presence of DI. The majority of COCP patients underwent transcranial surgery (77%) without receiving radiotherapy (84%). Compared with the 1Surg group, more CTrR patients developed visual field defects and ophthalmoplegia (all P < .01). Compared with patients without DI, those with DI had higher rates of anterior pituitary hormone deficits, body mass index, and fat mass (all P < .01). By contrast, fasting glucose, hemoglobin A1c, lipid panel, and quality of life were comparable among 1Surg vs CTrR patients, and patients with vs without DI. Regardless of primary treatment received, the presence of DI in either group was associated with higher rates of anterior pituitary hormone deficits and obesity. CTrR and DI predicted health outcomes differently. CTrR predisposed to the development of visual dysfunction, whereas DI was associated with higher rates of anterior pituitary dysfunction and weight gain. Higher body mass index and fat mass in patients with DI further implicate the role of hypothalamic damage as an important causal factor of obesity in these patients.

Value of platelet indices in identifying complete resolution of thrombus in deep venous thrombosis patients.

PubMed

Sevuk, Utkan; Altindag, Rojhat; Bahadir, Mehmet Veysi; Ay, Nurettin; Demirtas, Ertan; Ayaz, Fırat

2015-03-01

We aimed to evaluate whether mean platelet volume (MPV) and platelet distribution width (PDW) are helpful to identify complete thrombus resolution (CTR) after acute deep venous thrombosis (DVT). Patients who had first-time episode of acute proximal DVT were included in this retrospective study. 100 patients with DVT were divided into two groups according to absence (group 1; n = 68) or presence (group 2; n = 32) of CTR on doppler ultrasonography at month 6. There were no significant difference in admission MPV and PDW levels between group 1 and group 2. MPV (p = 0.03) and PDW (p < 0.001) levels at month 6 were significantly higher in group 1 than in group 2. CTR showed a moderate negative correlation with PDW at month 6 (ρ = -0.47) and a weak negative correlation with MPV at month 6 (ρ = -0.26). Logistic regression analysis showed that PDW (OR, 2.2; p = 0.004) at month 6 was an independent risk factor for the presence of residual venous thrombosis in DVT patients. Receiver operating characteristics analysis revealed that a 8.4 % decrease in admission MPV at month 6 provided 62 % sensitivity and 62 % specificity (AUC: 0.64) and a 15.4 % decrease in admission PDW at month 6 provided 87 % sensitivity and 94 % specificity (AUC: 0.89) for prediction of CTR in DVT patients. Percent change in admission MPV and PDW levels at month 6 may be used to identify the patients with CTR after a first episode of acute proximal DVT.

Introduction of a covalent histidine-heme linkage in a hemoglobin: a promising tool for heme protein engineering.

PubMed

Rice, Selena L; Preimesberger, Matthew R; Johnson, Eric A; Lecomte, Juliette T J

2014-12-01

The hemoglobins of the cyanobacteria Synechococcus and Synechocystis (GlbNs) are capable of spontaneous and irreversible attachment of the b heme to the protein matrix. The reaction, which saturates the heme 2-vinyl by addition of a histidine residue, is reproduced in vitro by preparing the recombinant apoprotein, adding ferric heme, and reducing the iron to the ferrous state. Spontaneous covalent attachment of the heme is potentially useful for protein engineering purposes. Thus, to explore whether the histidine-heme linkage can serve in such applications, we attempted to introduce it in a test protein. We selected as our target the heme domain of Chlamydomonas eugametos LI637 (CtrHb), a eukaryotic globin that exhibits less than 50% sequence identity with the cyanobacterial GlbNs. We chose two positions, 75 in the FG corner and 111 in the H helix, to situate a histidine near a vinyl group. We characterized the proteins with gel electrophoresis, absorbance spectroscopy, and NMR analysis. Both T111H and L75H CtrHbs reacted upon reduction of the ferric starting material containing cyanide as the distal ligand to the iron. With L75H CtrHb, nearly complete (>90%) crosslinking was observed to the 4-vinyl as expected from the X-ray structure of wild-type CtrHb. Reaction of T111H CtrHb also occurred at the 4-vinyl, in a 60% yield indicating a preference for the flipped heme orientation in the starting material. The work suggests that the His-heme modification will be applicable to the design of proteins with a non-dissociable heme group. Copyright © 2014 Elsevier Inc. All rights reserved.

7. EXTERIOR, SIDE VIEW FROM GARDEN SHOWING GRAPE ARBOR undated ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. EXTERIOR, SIDE VIEW FROM GARDEN SHOWING GRAPE ARBOR undated - Jean Baptiste Valle House, 99 South Main Street (Northwest corner of Main & Market Streets), Sainte Genevieve, Ste. Genevieve County, MO

20. STREAM ARBOR, LOOKING NORTHWEST Photocopy of photograph, 1930s National ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

20. STREAM ARBOR, LOOKING NORTHWEST Photocopy of photograph, 1930s National Park Service, National Capital Region files - Dumbarton Oaks Park, Thirty-second & R Streets Northwest, Washington, District of Columbia, DC

Nocturnal arboreality in snakes in the swamplands of the Atchafalaya Basin of south-central Louisiana and Big Thicket National Preserve of Southeast Texas

USGS Publications Warehouse

Glorioso, Brad M.; Waddle, J. Hardin

2017-01-01

The southeastern United States is home to a diverse assemblage of snakes, but only one species, the Rough Greensnake (Opheodrys aestivus), is considered specialized for a predominantly arboreal lifestyle. Other species, such as Ratsnakes (genus Pantherophis) and Ribbonsnakes/Gartersnakes (genus Thamnophis), are widely known to climb into vegetation and trees. Some explanations given for snake climbing behavior are foraging, thermoregulation, predator avoidance, and response to flood. Reports of arboreality in snake species typically not associated with life in the trees (such as terrestrial, aquatic, and even fossorial species) usually come from single observations, with no knowledge of prevalence of the behavior. Here, we report on arboreality of snake species detected during 8 years of night surveys in the Atchafalaya Basin of south-central Louisiana and 5+ years of night surveys in Big Thicket National Preserve in southeast Texas. We recorded a total of 1,088 detections of 19 snake species between the two study areas, with 348 detections above ground level (32%). The Rough Greensnake and Western Ribbonsnake (Thamnophis proximus) accounted for nearly 75% of total arboreal detections among the two study areas. However, with one exception, all snake species detected more than once between both study areas had at least one arboreal detection. These observations demonstrate that snakes with widely varying natural histories may be found in the trees at night, and for some species, this behavior may be more common than previously believed.

Action potentials reliably invade axonal arbors of rat neocortical neurons

PubMed Central

Cox, Charles L.; Denk, Winfried; Tank, David W.; Svoboda, Karel

2000-01-01

Neocortical pyramidal neurons have extensive axonal arborizations that make thousands of synapses. Action potentials can invade these arbors and cause calcium influx that is required for neurotransmitter release and excitation of postsynaptic targets. Thus, the regulation of action potential invasion in axonal branches might shape the spread of excitation in cortical neural networks. To measure the reliability and extent of action potential invasion into axonal arbors, we have used two-photon excitation laser scanning microscopy to directly image action-potential-mediated calcium influx in single varicosities of layer 2/3 pyramidal neurons in acute brain slices. Our data show that single action potentials or bursts of action potentials reliably invade axonal arbors over a range of developmental ages (postnatal 10–24 days) and temperatures (24°C-30°C). Hyperpolarizing current steps preceding action potential initiation, protocols that had previously been observed to produce failures of action potential propagation in cultured preparations, were ineffective in modulating the spread of action potentials in acute slices. Our data show that action potentials reliably invade the axonal arbors of neocortical pyramidal neurons. Failures in synaptic transmission must therefore originate downstream of action potential invasion. We also explored the function of modulators that inhibit presynaptic calcium influx. Consistent with previous studies, we find that adenosine reduces action-potential-mediated calcium influx in presynaptic terminals. This reduction was observed in all terminals tested, suggesting that some modulatory systems are expressed homogeneously in most terminals of the same neuron. PMID:10931955

Assessing arboreal adaptations of bird antecedents: testing the ecological setting of the origin of the avian flight stroke.

PubMed

Dececchi, T Alexander; Larsson, Hans C E

2011-01-01

The origin of avian flight is a classic macroevolutionary transition with research spanning over a century. Two competing models explaining this locomotory transition have been discussed for decades: ground up versus trees down. Although it is impossible to directly test either of these theories, it is possible to test one of the requirements for the trees-down model, that of an arboreal paravian. We test for arboreality in non-avian theropods and early birds with comparisons to extant avian, mammalian, and reptilian scansors and climbers using a comprehensive set of morphological characters. Non-avian theropods, including the small, feathered deinonychosaurs, and Archaeopteryx, consistently and significantly cluster with fully terrestrial extant mammals and ground-based birds, such as ratites. Basal birds, more advanced than Archaeopteryx, cluster with extant perching ground-foraging birds. Evolutionary trends immediately prior to the origin of birds indicate skeletal adaptations opposite that expected for arboreal climbers. Results reject an arboreal capacity for the avian stem lineage, thus lending no support for the trees-down model. Support for a fully terrestrial ecology and origin of the avian flight stroke has broad implications for the origin of powered flight for this clade. A terrestrial origin for the avian flight stroke challenges the need for an intermediate gliding phase, presents the best resolved series of the evolution of vertebrate powered flight, and may differ fundamentally from the origin of bat and pterosaur flight, whose antecedents have been postulated to have been arboreal and gliding.

Limited distal organelles and synaptic function in extensive monoaminergic innervation.

PubMed

Tao, Juan; Bulgari, Dinara; Deitcher, David L; Levitan, Edwin S

2017-08-01

Organelles such as neuropeptide-containing dense-core vesicles (DCVs) and mitochondria travel down axons to supply synaptic boutons. DCV distribution among en passant boutons in small axonal arbors is mediated by circulation with bidirectional capture. However, it is not known how organelles are distributed in extensive arbors associated with mammalian dopamine neuron vulnerability, and with volume transmission and neuromodulation by monoamines and neuropeptides. Therefore, we studied presynaptic organelle distribution in Drosophila octopamine neurons that innervate ∼20 muscles with ∼1500 boutons. Unlike in smaller arbors, distal boutons in these arbors contain fewer DCVs and mitochondria, although active zones are present. Absence of vesicle circulation is evident by proximal nascent DCV delivery, limited impact of retrograde transport and older distal DCVs. Traffic studies show that DCV axonal transport and synaptic capture are not scaled for extensive innervation, thus limiting distal delivery. Activity-induced synaptic endocytosis and synaptic neuropeptide release are also reduced distally. We propose that limits in organelle transport and synaptic capture compromise distal synapse maintenance and function in extensive axonal arbors, thereby affecting development, plasticity and vulnerability to neurodegenerative disease. © 2017. Published by The Company of Biologists Ltd.

Spatial complexity of carcass location influences vertebrate scavenger efficiency and species composition.

PubMed

Smith, Joshua B; Laatsch, Lauren J; Beasley, James C

2017-08-31

Scavenging plays an important role in shaping communities through inter- and intra-specific interactions. Although vertebrate scavenger efficiency and species composition is likely influenced by the spatial complexity of environments, heterogeneity in carrion distribution has largely been disregarded in scavenging studies. We tested this hypothesis by experimentally placing juvenile bird carcasses on the ground and in nests in trees to simulate scenarios of nestling bird carrion availability. We used cameras to record scavengers removing carcasses and elapsed time to removal. Carrion placed on the ground was scavenged by a greater diversity of vertebrates and at > 2 times the rate of arboreal carcasses, suggesting arboreal carrion may represent an important resource to invertebrate scavengers, particularly in landscapes with efficient vertebrate scavenging communities. Nonetheless, six vertebrate species scavenged arboreal carcasses. Rat snakes (Elaphe obsolete), which exclusively scavenged from trees, and turkey vultures (Cathartes aura) were the primary scavengers of arboreal carrion, suggesting such resources are potentially an important pathway of nutrient acquisition for some volant and scansorial vertebrates. Our results highlight the intricacy of carrion-derived food web linkages, and how consideration of spatial complexity in carcass distribution (i.e., arboreal) may reveal important pathways of nutrient acquisition by invertebrate and vertebrate scavenging guilds.

Serotonergic neurosecretory synapse targeting is controlled by Netrin-releasing guidepost neurons in C. elegans

PubMed Central

Nelson, Jessica C.; Colón-Ramos, Daniel A.

2013-01-01

Neurosecretory release sites lack distinct post-synaptic partners, yet target to specific circuits. This targeting specificity regulates local release of neurotransmitters and modulation of adjacent circuits. How neurosecretory release sites target to specific regions is not understood. Here we identify a molecular mechanism that governs the spatial specificity of extrasynaptic neurosecretory terminal formation in the serotonergic NSM neurons of C. elegans. We show that post-embryonic arborization and neurosecretory terminal targeting of the C. elegans NSM neuron is dependent on the Netrin receptor UNC-40/DCC. We observe that UNC-40 localizes to specific neurosecretory terminals at the time of axon arbor formation. This localization is dependent on UNC-6/Netrin, which is expressed by nerve ring neurons that act as guideposts to instruct local arbor and release site formation. We find that both UNC-34/Enabled and MIG-10/Lamellipodin are required downstream of UNC-40 to link the sites of ENT formation to nascent axon arbor extensions. Our findings provide a molecular link between release site development and axon arborization, and introduce a novel mechanism that governs the spatial specificity of serotonergic extrasynaptic neurosecretory terminals in vivo. PMID:23345213

Hemorrhage Control for Major Traumatic Vascular Injuries

DTIC Science & Technology

2015-10-01

Human Subjects Protection Scientist, at 301-619-9268/karen.m.eaton.ctr@mail.mil. SHARON A. EVANS, PhD, CIP Deputy Director, Human Research... CIP Deputy Director, Human Research Protection Office Office of Research Protections US Army Medical Research and Materiel Command Note: The...debra.depaul.ctr@mail.mil; the POC for the USAMRMC ORP HRPO review is the undersigned at 301-619-7801. 25 ANDREA J. KLINE, MS, CIP Director

National Positioning, Navigation, and Timing Architecture Study

DTIC Science & Technology

2008-09-01

Ballooning • Trip Planning Environment • Tide / Current Measures • BLM Tract Management • Oil Spill Containment • Hazardous Waste Remediation...SMC/GPSW (Aerospace) Adde, Barbara GOVT NASA Alexander, Dexter LTC USA SMDC Alexander, Ken GOVT FAA Rep to NCO Allen, Leonard GOVT FRA...Shawn Lt Col USAF NSSO/PNT Brewer, Mike Col USAF OASD/NII Space Programs Brodsky, Beryl CTR NASA (Overlook) Broussard, Robert CTR AFSPC/A5

‘A delicate diplomatic situation’: Tobacco industry efforts to gain control of the Framingham study

PubMed Central

Cataldo, J. K.; Bero, L. A.; Malone, R. E.

2010-01-01

The Framingham Heart Study (henceforth Framingham) is among the gold standards for epidemiological research. A prospective cohort study of 5,000+ men and women, it provided early findings about causes of coronary heart disease (CHD), following a cohort over 24 years. After US government funding ended, the tobacco industry-funded Council for Tobacco Research (CTR) provided continued funding for analyses related to smoking. Objective This study sought to understand the tobacco industry's motivation and activities in funding Framingham. Study Design We analyzed previously undisclosed tobacco industry documents, conducting iterative searches of the Legacy Tobacco Documents Library (http://legacy.library.ucsf.edu/), and assembled an historical case study. Results CTR funded Framingham to obtain full access to Framingham data. CTR planned for longtime industry consultant Carl Seltzer to reanalyze them to suggest that tobacco-related morbidity and mortality primarily resulted from “constitutional” factors such as age/ethnicity. Once data were obtained, CTR terminated funding for the Framingham principal investigator, who disagreed with Seltzer. Seltzer's critical analyses of subsequently-published work by the Framingham team created confusion about the association between coronary heart disease and cigarette smoking. Conclusion Researchers accepting tobacco industry funding risk losing control of data, analysis, and publication. PMID:20630333

Candida transmission and sexual behaviors as risks for a repeat episode of Candida vulvovaginitis.

PubMed

Reed, Barbara D; Zazove, Philip; Pierson, Carl L; Gorenflo, Daniel W; Horrocks, Julie

2003-12-01

To assess associations between female and male factors and the risk of recurring Candida vulvovaginitis. A prospective cohort study of 148 women with Candida vulvovaginitis and 78 of their male sexual partners was conducted at two primary care practices in the Ann Arbor, Michigan, area. Thirty-three of 148 women developed at least one further episode of Candida albicans vulvovaginitis within 1 year of follow-up. Cultures of Candida species from various sites of the woman (tongue, feces, vulva, and vagina) and from her partner (tongue, feces, urine, and semen) did not predict recurrences. Female factors associated with recurrence included recent masturbating with saliva (hazard ratio 2.66 [95% CI 1.17-6.06]) or cunnilingus (hazard ratio 2.94 [95% CI 1.12-7.68]) and ingestion of two or more servings of bread per day (p

Intestinal diffuse large B-cell lymphoma: an evaluation of different staging systems.

PubMed

Hwang, Hee Sang; Yoon, Dok Hyun; Suh, Cheolwon; Park, Chan-Sik; Huh, Jooryung

2014-01-01

The gastrointestinal tract is the most common primary extranodal site for diffuse large B-cell lymphoma (DLBCL). However, there is no consensus on the most appropriate staging system for intestinal DLBCL. We evaluated the utility of the modified Ann Arbor system, the Lugano system, and the Paris staging system (a modification of the Tumor, Node, Metastases [TNM] staging for epithelial tumors) in 66 cases of resected intestinal DLBCL. The cases were treated with surgery, plus either cyclophosphamide, doxorubicin, vincristine, and prednisolone (CHOP) chemotherapy alone (n=26) or with the addition of rituximab immunotherapy (n=40). Median follow-up time was 40.4 months (range, 2.1-171.6 months). Fifty-six patients (84.8%) achieved complete remission. The overall 5-yr survival rate was 86.4% (57/66). Of the stage categories defined for each staging system, only the T stage of the Paris classification showed prognostic significance for overall survival by univariate analysis. However, none of the stage parameters was significantly correlated with patient survival on multivariate analysis. In conclusion, the results suggest that the T stage of the Paris classification system may be a prognostic indicator in intestinal DLBCL. The results also imply that in surgically resected intestinal DLBCL, the addition of rituximab to the CHOP regimen does not confer significant survival advantage.

Thermospheric wind effects on the global distribution of helium in the earth's upper atmosphere. Ph.D. Thesis - Michigan Univ., Ann Arbor

NASA Technical Reports Server (NTRS)

Reber, C. A.

1973-01-01

The momentum and continuity equations for a minor gas are combined with the momentum equation for the major constituents to obtain the time dependent continuity equation for the minor species reflecting a wind field in the background gas. This equation is used to study the distributions of helium and argon at times of low, medium, and high solar activity for a variety of latitudinal-seasonal wind cells. For helium, the exospheric return flow at the higher thermospheric temperatures dominates the distribution to the extent that much larger latitudinal gradients can be maintained during periods of low solar activity than during periods of high activity. By comparison to the exospheric flow, the smoothing effect of horizontal diffusion is almost negligible. The latitudinal variation of helium observed by satellite mass spectrometers can be reproduced by the effect of a wind system of air rising in the summer hemisphere, flowing across the equator with speeds on the order of 100 to 200 m/sec, and descending in the winter hemisphere. Argon, being heavier than the mean mass in the lower thermosphere, reacts oppositely to helium in that it is enhanced in the summer hemisphere and depleted in the winter.

Remote sensing of changes in morphology and physiology of trees under stress

NASA Technical Reports Server (NTRS)

Olson, C. E., Jr.; Rohde, W. G.; Ward, J. M.

1970-01-01

Results of continuing studies of forest trees subjected to varying types of stress are reported. Both greenhouse and field studies are included. Greenhouse work with tree seedlings exposed to varying levels of NaCl and CaCl2 in the soil indicated that, in the initial stages, palisade cells shrink and the amount of air space in the leaf increases. As the severity of damage increases, the cells of the spongy mesophyll shrink and flatten, and the amount of air space in the leaf decreases. Statistical analysis of foliar reflectance and associated moisture content data led to a series of regression equations for predicting foliar moisture content from reflectance data. Equations were calculated for three species, yellow birch (Betula alleghaniensis Britton), sugar maple (Acer saccharum Marsh.) and white ash (Fraxinus americana L.) having multiple correlation coefficients of 0.98, 0.94 and 0.93 respectively. Interpretation of multispectral imagery of the Ann Arbor Forestry Test Site (NASA Site 190) provided evidence that infections of Fomes annosus can be detected in the early stages. Infections of two needle cast diseases were also detected in conifer plantations in the test site. A study of automatic interpretation of multispectral scanner imagery for tree species recognition provided encouraging results.

Disparities in survival after Hodgkin lymphoma: a population-based study

PubMed Central

Keegan, Theresa H.M.; Clarke, Christina A.; Chang, Ellen T.; Shema, Sarah J.; Glaser, Sally L.

2009-01-01

Survival after Hodgkin lymphoma (HL) is generally favorable, but may vary by patient demographic characteristics. The authors examined HL survival according to race/ethnicity and neighborhood socioeconomic status (SES), determined from residential census block group at diagnosis. For 12,492 classical HL patients ≥15 years diagnosed in California during 1988-2006 and followed through 2007, we determined risk of overall and HL-specific death using Cox proportional hazards regression; analyses were stratified by age and Ann Arbor stage. Irrespective of disease stage, patients with lower neighborhood SES had worse overall and HL-specific survival than patients with higher SES. Patients with the lowest quintile of neighborhood SES had a 64% (patients aged 15-44 years) and 36% (≥45 years) increased risk of HL-death compared to patients with the highest quintile of SES; SES results were similar for overall survival. Even after adjustment for neighborhood SES, blacks and Hispanics had increased risks of HL-death 74% and 43% (15-44 years) and 40% and 17% (≥45 years), respectively, higher than white patients. The racial/ethnic differences in survival were evident for all stages of disease. These data provide evidence for substantial, and probably remediable, racial/ethnic and neighborhood SES disparities in HL outcomes. PMID:19557531

Stillbirth in week 19 of pregnancy followed by maternal death as a consequence of refused chemotherapy for non-hodgkin's lymphoma--significance of adjuvant chemotherapy in women of reproductive age.

PubMed

Hauenstein, Evelyn; Seidl, Stefan; Schneider, Karl T M; Fischer, Thorsten

2010-01-01

Due to rising cure rates in cancer, the question of preserving fertility in young female patients becomes more important. Especially in lymphomas, incidence and long-time survival have increased. Hematologists and gynecologists have to treat more and more female patients who wish to become pregnant despite their disease and/or after finishing treatment. We report on a 28-year-old patient with highly malignant non-Hodgkin's lymphoma (peripheral T cell lymphoma, Ann Arbor stage IV) and main manifestation at the gastric antrum, with a distinct wish for becoming pregnant. Chemotherapy was strongly recommended to her, but she refused. After she had conceived, the disease recurred, followed by stillbirth in week 19 of gestation and death due to gastric perforation and septic shock. Facing the risk of sterility after chemotherapy should not induce patients to refuse chemotherapy and risk their lives. Treatment of young female cancer patients should therefore always include a thorough discussion about other ways of preserving fertility for the time after treatment. Such strategies exist, although their success is still limited and not every patient is eligible for them. Copyright © 2010 S. Karger AG, Basel.

Glucose tolerance in two unacculturated Indian tribes of Brazil.

PubMed

Spielman, R S; Fajans, S S; Neel, J V; Pek, S; Floyd, J C; Oliver, W J

1982-08-01

Plasma levels of glucose, insulin, growth hormone, and pancreatic polypeptide in response to a standard oral glucose load were studied in the Yanomama and the Marubo, two relatively unacculturated Amerindian tribes of the Brazilian Amazon. The findings in the two tribes differed significantly from each other and in the degree of deviation from control subjects. The average responses in both tribes differed significantly from those of age- and sex-matched Caucasoid control subjects studied in Ann Arbor, Michigan; however, of the two tribes, the Marubo, the more acculturated group, resembled the controls more closely. Plasma concentrations of glucose and the hormones at three time points (fasting, 1 h, 2 h) were compared by means of a multivariate analysis. When the Marubo were compared with the control subjects, the only highly significant difference was in the plasma glucose concentrations (all three points were higher in the Marubo); however, the Yanomama differed significantly from the control subjects with respect to all four plasma indicators (p less than 0.05). Unlike the Marubo, the Yanomama showed no significant rise in plasma glucose at 1 h and no decrease at 2 h. Neither tribe exhibited the bimodality of the 2 h glucose value characteristic of acculturated Amerindians, such as the Pima, but the samples studied were small.

Evaluation of the TRPM2 channel as a biomarker in breast cancer using public databases analysis.

PubMed

Sumoza-Toledo, Adriana; Espinoza-Gabriel, Mario Iván; Montiel-Condado, Dvorak

Breast cancer is one of the most common malignancies affecting women. Recent investigations have revealed a major role of ion channels in cancer. The transient receptor potential melastatin-2 (TRPM2) is a plasma membrane and lysosomal channel with important roles in cell migration and cell death in immune cells and tumor cells. In this study, we investigated the prognostic value of TRPM2 channel in breast cancer, analyzing public databases compiled in Oncomine™ (Thermo Fisher, Ann Arbor, MI) and online Kaplan-Meier Plotter platforms. The results revealed that TRPM2 mRNA overexpression is significant in situ and invasive breast carcinoma compared to normal breast tissue. Furthermore, multi-gene validation using Oncomine™ showed that this channel is coexpressed with proteins related to cellular migration, transformation, and apoptosis. On the other hand, Kaplan-Meier analysis exhibited that low expression of TRPM2 could be used to predict poor outcome in ER- and HER2+ breast carcinoma patients. TRPM2 is a promising biomarker for aggressiveness of breast cancer, and a potential target for the development of new therapies. Copyright © 2016 Hospital Infantil de México Federico Gómez. Publicado por Masson Doyma México S.A. All rights reserved.

Workshop summary: connecting social and environmental factors to measure and track environmental health disparities.

PubMed

Payne-Sturges, Devon; Gee, Gilbert C; Crowder, Kirstin; Hurley, Bradford J; Lee, Charles; Morello-Frosch, Rachel; Rosenbaum, Arlene; Schulz, Amy; Wells, Charles; Woodruff, Tracey; Zenick, Hal

2006-10-01

On May 24-25, 2005 in Ann Arbor, Michigan, the US Environmental Protection Agency, the National Institute of Environmental Health Sciences, and the University of Michigan sponsored a technical workshop on the topic of connecting social and environmental factors to measure and track environmental health disparities. The workshop was designed to develop a transdisciplinary scientific foundation for exploring the conceptual issues, data needs, and policy applications associated with social and environmental factors used to measure and track racial, ethnic, and class disparities in environmental health. Papers, presentations, and discussions focused on the use of multilevel analysis to study environmental health disparities, the development of an organizing framework for evaluating health disparities, the development of indicators, and the generation of community-based participatory approaches for indicator development and use. Group exercises were conducted to identify preliminary lists of priority health outcomes and potential indicators and to discuss policy implications and next steps. Three critical issues that stem from the workshop were: (a) stronger funding support is needed for community-based participatory research in environmental health disparities, (b) race/ethnicity and socioeconomic position need to be included in environmental health surveillance and research, and (c) models to elucidate the interrelations between social, physical, and built environments should continue to be developed and empirically tested.

The Michigan Binary Star Program

NASA Astrophysics Data System (ADS)

Lindner, Rudi P.

2007-07-01

At the end of the nineteenth century, William J. Hussey and Robert G. Aitken, both at Lick Observatory, began a systematic search for unrecorded binary stars with the aid of the 12" and 36" refracting telescopes at Lick Observatory. Aitken's work (and book on binary stars) are well known, Hussey's contributions less so. In 1905 Hussey, a Michigan engineering graduate, returned to direct the Ann Arbor astronomy program, and immediately he began to design new instrumentation for the study of binary stars and to train potential observers. For a time, he spent six months a year at the La Plata Observatory, where he discovered a number of new pairs and decided upon a major southern hemisphere campaign. He spent a decade obtaining the lenses for a large refractor, through the vicissitudes of war and depression. Finally, he obtained a site in South Africa, a 26" refractor, and a small corps of observers, but he died in London en route to fulfill his dream. His right hand man, Richard Rossiter, established the observatory and spent the next thirty years discovering and measuring binary stars: his personal total is a record for the field. This talk is an account of the methods, results, and utility of the extraordinary binary star factory in the veldt.

The influence of the multi-basic cleavage site of the H5 hemagglutinin on the attenuation, immunogenicity and efficacy of a live attenuated influenza A H5N1 cold-adapted vaccine virus.

PubMed

Suguitan, Amorsolo L; Marino, Michael P; Desai, Purvi D; Chen, Li-Mei; Matsuoka, Yumiko; Donis, Ruben O; Jin, Hong; Swayne, David E; Kemble, George; Subbarao, Kanta

2009-12-20

A recombinant live attenuated influenza virus DeltaH5N1 vaccine with a modified hemagglutinin (HA) and intact neuraminidase genes from A/Vietnam/1203/04 (H5N1) and six remaining genome segments from A/Ann Arbor/6/60 (H2N2) cold-adapted (AA ca) virus was previously shown to be attenuated in chickens, mice and ferrets. Evaluation of the recombinant H5N1 viruses in mice indicated that three independent factors contributed to the attenuation of the DeltaH5N1 vaccine: the attenuating mutations specified by the AA ca loci had the greatest influence, followed by the deletion of the H5 HA multi-basic cleavage site (MBS), and the constellation effects of the AA genes acting in concert with the H5N1 glycoproteins. Restoring the MBS in the H5 HA of the vaccine virus improved its immunogenicity and efficacy, likely as a consequence of increased virus replication, indicating that removal of the MBS had a deleterious effect on the immunogenicity and efficacy of the DeltaH5N1 vaccine in mice.

Christopher M. Peterson (1950-2012).

PubMed

Park, Nansook; Seligman, Martin E P

2013-01-01

Presents an obituary for Christopher M. Peterson. "Other people matter. Period," said Christopher M. Peterson when asked for a concise definition of "positive psychology," the field he helped to found and then helped to guide through the first decade of the 21st century. He researched, taught, and lived positive psychology. When Chris died prematurely on October 9, 2012, in Ann Arbor, Michigan, from sudden heart failure, the world lost a distinguished scholar, an inspirational teacher, and a wonderful human being. Chris loved people. He was genuinely interested in getting to know others. He made everyone around him feel comfortable and respected. An understated, humble, and generous person, it was no accident that his nickname was "Mother Theresa." He always found joy in letting other people shine and sharing the credit with them. He had a gift for seeing the best in each person. He also had a strong sense of fairness and duty without being rigid or allowing his personal feelings to obscure his judgment. He had fun whatever he did and brought fun for others. Although his scholarly contributions are significant, his lasting legacy will be what he shared with other people. So many of us are indebted to him for making us better researchers, better teachers, and better people. (PsycINFO Database Record (c) 2013 APA, all rights reserved).

The influence of the multi-basic cleavage site of the H5 hemagglutinin on the attenuation, immunogenicity and efficacy of a live attenuated influenza A H5N1 cold-adapted vaccine virus

PubMed Central

Suguitan, Amorsolo L.; Marino, Michael P.; Desai, Purvi D.; Chen, Li-Mei; Matsuoka, Yumiko; Donis, Ruben O.; Jin, Hong; Swayne, David E.; Kemble, George; Subbarao, Kanta

2009-01-01

A recombinant live attenuated influenza virus ΔH5N1 vaccine with a modified hemagglutinin (HA) and intact neuraminidase genes from A/Vietnam/1203/04 (H5N1) and six remaining genome segments from A/Ann Arbor/6/60 (H2N2) cold-adapted (AA ca) virus was previously shown to be attenuated in chickens, mice and ferrets. Evaluation of the recombinant H5N1 viruses in mice indicated that three independent factors contributed to the attenuation of the ΔH5N1 vaccine: the attenuating mutations specified by the AA ca loci had the greatest influence, followed by the deletion of the H5 HA multi-basic cleavage site (MBS), and the constellation effects of the AA genes acting in concert with the H5N1 glycoproteins. Restoring the MBS in the H5 HA of the vaccine virus improved its immunogenicity and efficacy, likely as a consequence of increased virus replication, indicating that removal of the MBS had a deleterious effect on the immunogenicity and efficacy of the ΔH5N1 vaccine in mice. PMID:19833372

Manual and Flow-Injection Detection/Quantification of Polyquaterniums via Fully Reversible Polyion-Sensitive Polymeric Membrane-Based Ion-Selective Electrodes.

PubMed

Ferguson, Stephen A; Meyerhoff, Mark E

2017-10-27

The detection of four different polyquaterniums (PQs) using a fully reversible potentiometric polyion sensor in three different detection modes is described. The polyion sensing "pulstrodes" serve as the detector for direct dose-response experiments, beaker titrations, and in a flow-injection analysis (FIA) system. Direct polycation response toward PQ-2, PQ-6, PQ-10, and poly(2-methacryloxyethyltrimethylammonium) chloride (PMETAC) yields characteristic information about each PQ species (e.g., relative charge densities, etc.) via syringe pump addition of each PQ species to a background electrolyte solution. Quantitative titrations are performed using a syringe pump to deliver heparin as the polyanion titrant to quantify all four PQs at μg/mL levels. Both the direct and indirect methods incorporate the use of a three-electrode system including counter, double junction reference, and working electrodes. The working electrode possesses a plasticized poly(vinyl chloride) (PVC) membrane containing the neutral lipophilic salt of dinonylnaphthalenesulfonate (DNNS - ) tridodecylmethylammonium (TDMA + ). Further, the titration method is shown to be useful to quantify PQ-6 levels in recreational swimming pool water collected in Ann Arbor, MI. Finally, a FIA system equipped with a pulstrode detector is used to demonstrate the ability to potentially quantify PQ levels via a more streamlined and semiautomated testing platform.

A geothermal AMTEC system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schuller, M.J.; LeMire, R.A.; Horner-Richardson, K.

1995-12-31

The Phillips Laboratory Power and Thermal Management Division (PL/VTP), with the support of ORION International Technologies, is investigating new methods of advanced thermal to electric power conversion for space and terrestrial applications. The alkali metal thermal-to-electric converter (AMTEC), manufactured primarily by Advanced Modular Power Systems (AMPS) of Ann Arbor, MI, has reached a level of technological maturity which would allow its use in a constant, unattended thermal source, such as a geothermal field. Approximately 95,000 square miles in the western United States has hot dry rock with thermal gradients of 60 C/km and higher. Several places in the United Statesmore » and the world have thermal gradients of 500 C/km. Such heat sources represent an excellent thermal source for a system of modular power units using AMTEC devices to convert the heat to electricity. AMTEC cells using sodium as a working fluid require heat input at temperatures between 500 and 1,000 C to generate power. The present state of the art is capable of 15% efficiency with 800 C heat input and has demonstrated 18% efficiency for single cells. This paper discusses the basics of AMTEC operation, current drilling technology as a cost driver, design of modular AMTEC power units, heat rejection technologies, materials considerations, and estimates of power production from a geothermal AMTEC concept.« less

High Resolution Sensing and Control of Urban Water Networks

NASA Astrophysics Data System (ADS)

Bartos, M. D.; Wong, B. P.; Kerkez, B.

2016-12-01

We present a framework to enable high-resolution sensing, modeling, and control of urban watersheds using (i) a distributed sensor network based on low-cost cellular-enabled motes, (ii) hydraulic models powered by a cloud computing infrastructure, and (iii) automated actuation valves that allow infrastructure to be controlled in real time. This platform initiates two major advances. First, we achieve a high density of measurements in urban environments, with an anticipated 40+ sensors over each urban area of interest. In addition to new measurements, we also illustrate the design and evaluation of a "smart" control system for real-world hydraulic networks. This control system improves water quality and mitigates flooding by using real-time hydraulic models to adaptively control releases from retention basins. We evaluate the potential of this platform through two ongoing deployments: (i) a flood monitoring network in the Dallas-Fort Worth metropolitan area that detects and anticipates floods at the level of individual roadways, and (ii) a real-time hydraulic control system in the city of Ann Arbor, MI—soon to be one of the most densely instrumented urban watersheds in the United States. Through these applications, we demonstrate that distributed sensing and control of water infrastructure can improve flash flood predictions, emergency response, and stormwater contaminant mitigation.

Dynamics of terminal arbor formation and target approach of retinotectal axons in living zebrafish embryos: a time-lapse study of single axons.

PubMed

Kaethner, R J; Stuermer, C A

1992-08-01

In a variety of species, developing retinal axons branch initially more widely in their visual target centers and only gradually restrict their terminal arbors to smaller and defined territories. Retinotectal axons in fish, however, appeared to grow in a directed manner and to arborize only at their retinotopic target sites. To visualize the dynamics of retinal axon growth and arbor formation in fish, time-lapse recordings were made of individual retinal ganglion cell axons in the tectum in live zebrafish embryos. Axons were labeled with the fluorescent carbocyanine dyes Dil or DiO inserted as crystals into defined regions of the retina, viewed with 40x and 100x objectives with an SIT camera, and recorded, with exposure times of 200 msec at 30 or 60 sec intervals, over time periods of up to 13 hr. (1) Growth cones advanced rapidly, but the advance was punctuated by periods of rest. During the rest periods, the growth cones broadened and developed filopodia, but during extension they were more streamlined. (2) Growth cones traveled unerringly into the direction of their retinotopic targets without branching en route. At their target and only there, the axons began to form terminal arborizations, a process that involved the emission and retraction of numerous short side branches. The area that was permanently occupied or touched by transient branches of the terminal arbor--"the exploration field"--was small and almost circular and covered not more than 5.3% of the entire tectal surface area, but represented up to six times the size of the arbor at any one time. These findings are consistent with the idea that retinal axons are guided to their retinotopic target sites by sets of positional markers, with a graded distribution over the axes of the tectum.

22. MEADOW, LOOKING EAST WITH STREAM ARBOR ON RIGHT Photocopy ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

22. MEADOW, LOOKING EAST WITH STREAM ARBOR ON RIGHT Photocopy of photograph, 1930s National Park Service, National Capital Region files - Dumbarton Oaks Park, Thirty-second & R Streets Northwest, Washington, District of Columbia, DC

Habitat use affects morphological diversification in dragon lizards

PubMed Central

COLLAR, D C; SCHULTE, J A; O’MEARA, B C; LOSOS, J B

2010-01-01

Habitat use may lead to variation in diversity among evolutionary lineages because habitats differ in the variety of ways they allow for species to make a living. Here, we show that structural habitats contribute to differential diversification of limb and body form in dragon lizards (Agamidae). Based on phylogenetic analysis and ancestral state reconstructions for 90 species, we find that multiple lineages have independently adopted each of four habitat use types: rock-dwelling, terrestriality, semi-arboreality and arboreality. Given these reconstructions, we fit models of evolution to species’ morphological trait values and find that rock-dwelling and arboreality limit diversification relative to terrestriality and semi-arboreality. Models preferred by Akaike information criterion infer slower rates of size and shape evolution in lineages inferred to occupy rocks and trees, and model-averaged rate estimates are slowest for these habitat types. These results suggest that ground-dwelling facilitates ecomorphological differentiation and that use of trees or rocks impedes diversification. PMID:20345808

Visualization of Motor Axon Navigation and Quantification of Axon Arborization In Mouse Embryos Using Light Sheet Fluorescence Microscopy.

PubMed

Liau, Ee Shan; Yen, Ya-Ping; Chen, Jun-An

2018-05-11

Spinal motor neurons (MNs) extend their axons to communicate with their innervating targets, thereby controlling movement and complex tasks in vertebrates. Thus, it is critical to uncover the molecular mechanisms of how motor axons navigate to, arborize, and innervate their peripheral muscle targets during development and degeneration. Although transgenic Hb9::GFP mouse lines have long served to visualize motor axon trajectories during embryonic development, detailed descriptions of the full spectrum of axon terminal arborization remain incomplete due to the pattern complexity and limitations of current optical microscopy. Here, we describe an improved protocol that combines light sheet fluorescence microscopy (LSFM) and robust image analysis to qualitatively and quantitatively visualize developing motor axons. This system can be easily adopted to cross genetic mutants or MN disease models with Hb9::GFP lines, revealing novel molecular mechanisms that lead to defects in motor axon navigation and arborization.

Assessing Arboreal Adaptations of Bird Antecedents: Testing the Ecological Setting of the Origin of the Avian Flight Stroke

PubMed Central

Dececchi, T. Alexander; Larsson, Hans C. E.

2011-01-01

The origin of avian flight is a classic macroevolutionary transition with research spanning over a century. Two competing models explaining this locomotory transition have been discussed for decades: ground up versus trees down. Although it is impossible to directly test either of these theories, it is possible to test one of the requirements for the trees-down model, that of an arboreal paravian. We test for arboreality in non-avian theropods and early birds with comparisons to extant avian, mammalian, and reptilian scansors and climbers using a comprehensive set of morphological characters. Non-avian theropods, including the small, feathered deinonychosaurs, and Archaeopteryx, consistently and significantly cluster with fully terrestrial extant mammals and ground-based birds, such as ratites. Basal birds, more advanced than Archaeopteryx, cluster with extant perching ground-foraging birds. Evolutionary trends immediately prior to the origin of birds indicate skeletal adaptations opposite that expected for arboreal climbers. Results reject an arboreal capacity for the avian stem lineage, thus lending no support for the trees-down model. Support for a fully terrestrial ecology and origin of the avian flight stroke has broad implications for the origin of powered flight for this clade. A terrestrial origin for the avian flight stroke challenges the need for an intermediate gliding phase, presents the best resolved series of the evolution of vertebrate powered flight, and may differ fundamentally from the origin of bat and pterosaur flight, whose antecedents have been postulated to have been arboreal and gliding. PMID:21857918

Slow but tenacious: an analysis of running and gripping performance in chameleons.

PubMed

Herrel, Anthony; Tolley, Krystal A; Measey, G John; da Silva, Jessica M; Potgieter, Daniel F; Boller, Elodie; Boistel, Renaud; Vanhooydonck, Bieke

2013-03-15

Chameleons are highly specialized and mostly arboreal lizards characterized by a suite of derived characters. The grasping feet and tail are thought to be related to the arboreal lifestyle of chameleons, yet specializations for grasping are thought to exhibit a trade-off with running ability. Indeed, previous studies have demonstrated a trade-off between running and clinging performance, with faster species being poorer clingers. Here we investigate the presence of trade-offs by measuring running and grasping performance in four species of chameleon belonging to two different clades (Chamaeleo and Bradypodion). Within each clade we selected a largely terrestrial species and a more arboreal species to test whether morphology and performance are related to habitat use. Our results show that habitat drives the evolution of morphology and performance but that some of these effects are specific to each clade. Terrestrial species in both clades show poorer grasping performance than more arboreal species and have smaller hands. Moreover, hand size best predicts gripping performance, suggesting that habitat use drives the evolution of hand morphology through its effects on performance. Arboreal species also had longer tails and better tail gripping performance. No differences in sprint speed were observed between the two Chamaeleo species. Within Bradypodion, differences in sprint speed were significant after correcting for body size, yet the arboreal species were both better sprinters and had greater clinging strength. These results suggest that previously documented trade-offs may have been caused by differences between clades (i.e. a phylogenetic effect) rather than by design conflicts between running and gripping per se.

Hand pressures during arboreal locomotion in captive bonobos (Pan paniscus).

PubMed

Samuel, Diana S; Nauwelaerts, Sandra; Stevens, Jeroen M G; Kivell, Tracy L

2018-04-19

Evolution of the human hand has undergone a transition from use during locomotion to use primarily for manipulation. Previous comparative morphological and biomechanical studies have focused on potential changes in manipulative abilities during human hand evolution, but few have focused on functional signals for arboreal locomotion. Here, we provide this comparative context though the first analysis of hand loading in captive bonobos during arboreal locomotion. We quantify pressure experienced by the fingers, palm and thumb in bonobos during vertical locomotion, suspension and arboreal knuckle-walking. The results show that pressure experienced by the fingers is significantly higher during knuckle-walking compared with similar pressures experienced by the fingers and palm during suspensory and vertical locomotion. Peak pressure is most often experienced at or around the third digit in all locomotor modes. Pressure quantified for the thumb is either very low or absent, despite the thumb making contact with the substrate during all suspensory and vertical locomotor trials. Unlike chimpanzees, bonobos do not show a rolling pattern of digit contact with the substrate during arboreal knuckle-walking - instead, we found that digits 3 and 4 typically touch down first and digit 5 almost always made contact with the substrate. These results have implications for interpreting extant and fossilized hand morphology; we expect bonobo (and chimpanzee) bony morphology to primarily reflect the biomechanical loading of knuckle-walking, while functional signals for arboreal locomotion in fossil hominins are most likely to appear in the fingers, particularly digit 3, and least likely to appear in the morphology of the thumb. © 2018. Published by The Company of Biologists Ltd.

Coherent Transition Radiation Generated from Transverse Electron Density Modulation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Halavanau, A.; Piot, P.; Tyukhtin, A. V.

Coherent Transition radiation (CTR) of a given frequency is commonly generated with longitudinal electron bunch trains. In this paper, we present a study of CTR properties produced from simultaneous electron transverse and longitudinal density modulation. We demonstrate via numerical simulations a simple technique to generate THz-scale frequencies from mm-scale transversely separated electron beamlets formed into a ps-scale bunch train. The results and a potential experimental setup are discussed.