Sample records for cultural differences affect

  1. Cultural Differences in Affect Intensity Perception in the Context of Advertising

    PubMed Central

    Pogosyan, Marianna; Engelmann, Jan B.

    2011-01-01

    Cultural differences in the perception of positive affect intensity within an advertising context were investigated among American, Japanese, and Russian participants. Participants were asked to rate the intensity of facial expressions of positive emotions, which displayed either subtle, low intensity, or salient, high intensity expressions of positive affect. In agreement with previous findings from cross-cultural psychological research, current results demonstrate both cross-cultural agreement and differences in the perception of positive affect intensity across the three cultures. Specifically, American participants perceived high arousal (HA) images as significantly less calm than participants from the other two cultures, while the Japanese participants perceived low arousal (LA) images as significantly more excited than participants from the other cultures. The underlying mechanisms of these cultural differences were further investigated through difference scores that probed for cultural differences in perception and categorization of positive emotions. Findings indicate that rating differences are due to (1) perceptual differences in the extent to which HA images were discriminated from LA images, and (2) categorization differences in the extent to which facial expressions were grouped into affect intensity categories. Specifically, American participants revealed significantly higher perceptual differentiation between arousal levels of facial expressions in high and intermediate intensity categories. Japanese participants, on the other hand, did not discriminate between high and low arousal affect categories to the same extent as did the American and Russian participants. These findings indicate the presence of cultural differences in underlying decoding mechanisms of facial expressions of positive affect intensity. Implications of these results for global advertising are discussed. PMID:22084635

  2. Cultural differences in affect intensity perception in the context of advertising.

    PubMed

    Pogosyan, Marianna; Engelmann, Jan B

    2011-01-01

    Cultural differences in the perception of positive affect intensity within an advertising context were investigated among American, Japanese, and Russian participants. Participants were asked to rate the intensity of facial expressions of positive emotions, which displayed either subtle, low intensity, or salient, high intensity expressions of positive affect. In agreement with previous findings from cross-cultural psychological research, current results demonstrate both cross-cultural agreement and differences in the perception of positive affect intensity across the three cultures. Specifically, American participants perceived high arousal (HA) images as significantly less calm than participants from the other two cultures, while the Japanese participants perceived low arousal (LA) images as significantly more excited than participants from the other cultures. The underlying mechanisms of these cultural differences were further investigated through difference scores that probed for cultural differences in perception and categorization of positive emotions. Findings indicate that rating differences are due to (1) perceptual differences in the extent to which HA images were discriminated from LA images, and (2) categorization differences in the extent to which facial expressions were grouped into affect intensity categories. Specifically, American participants revealed significantly higher perceptual differentiation between arousal levels of facial expressions in high and intermediate intensity categories. Japanese participants, on the other hand, did not discriminate between high and low arousal affect categories to the same extent as did the American and Russian participants. These findings indicate the presence of cultural differences in underlying decoding mechanisms of facial expressions of positive affect intensity. Implications of these results for global advertising are discussed.

  3. Culture Wires the Brain: A Cognitive Neuroscience Perspective

    PubMed Central

    Park, Denise C.; Huang, Chih-Mao

    2012-01-01

    There is clear evidence that sustained experiences may affect both brain structure and function. Thus, it is quite reasonable to posit that sustained exposure to a set of cultural experiences and behavioral practices will affect neural structure and function. The burgeoning field of cultural psychology has often demonstrated the subtle differences in the way individuals process information—differences that appear to be a product of cultural experiences. We review evidence that the collectivistic and individualistic biases of East Asian and Western cultures, respectively, affect neural structure and function. We conclude that there is limited evidence that cultural experiences affect brain structure and considerably more evidence that neural function is affected by culture, particularly activations in ventral visual cortex—areas associated with perceptual processing. PMID:22866061

  4. Depressive symptoms and positive affect in Chinese and United States breast cancer survivors: a cross-cultural comparison.

    PubMed

    Milbury, Kathrin; Kavanagh, April; Meng, Zhiqiang; Chen, Zhen; Chandwani, Kavita D; Garcia, Kay; Perkins, George H; McQuade, Jennifer; Raghuram, Nelamangala V; Nagarathna, Raghuram; Liao, Zhongxing; Nagendra, Hongasandra Ramarao; Chen, Jiayi; Guo, Xiaoma; Liu, Luming; Arun, Banu; Cohen, Lorenzo

    2017-07-01

    Research in the area of cultural response pattern on questionnaires in the oncological setting and direct cross-cultural comparisons are lacking. This study examined response pattern in the reporting of depressive symptoms in Chinese and US women with breast cancer. We hypothesized that Chinese women are less likely to endorse positive affect items compared to their US counterparts. Additionally, we explored cultural differences in the association between positive affect and QOL. Secondary analyses of baseline assessments of two mind-body intervention studies for women with breast cancer undergoing radiotherapy in the USA (N = 62) and China (N = 97) are presented. All participants completed measures of depressive symptoms (CES-D) and cancer-specific QOL (FACT-B). We examined cultural differences on positive and negative affect items on the CES-D. Controlling for demographic factors, ANCOVA revealed a significant cultural difference in positive (F = 7.99, p = 0.005) but not negative affect (p = 0.82) with Chinese women reporting lower positive affect compared to US women (Chinese = 6.97 vs. US = 8.31). There was also a significant cultural difference (F = 3.94, p = 0.03) in the association between positive affect and QOL so that lower positive affect was more strongly associated with worse emotional well-being in Chinese (beta = 0.57, p < 0.0001) than US women (beta = 0.35, p < 0.01). Chinese women reported lower positive affect compared to US women and lower levels of positive affect were more strongly associated with worse QOL. Special attention is needed when examining mental health in different cultures to ascertain effective delivery of clinical services to those in need.

  5. Depressive symptoms and positive affect in Chinese and United States breast cancer survivors: a cross-cultural comparison

    PubMed Central

    Milbury, Kathrin; Kavanagh, April; Meng, Zhiqiang; Chen, Zhen; Chandwani, Kavita D.; Garcia, Kay; Perkins, George H.; McQuade, Jennifer; Raghuram, Nelamangala V.; Nagarathna, Raghuram; Liao, Zhongxing; Nagendra, Hongasandra Ramarao; Chen, Jiayi; Guo, Xiaoma; Liu, Luming; Arun, Banu

    2018-01-01

    Purpose Research in the area of cultural response pattern on questionnaires in the oncological setting and direct cross-cultural comparisons are lacking. This study examined response pattern in the reporting of depressive symptoms in Chinese and US women with breast cancer. We hypothesized that Chinese women are less likely to endorse positive affect items compared to their US counterparts. Additionally, we explored cultural differences in the association between positive affect and QOL. Methods Secondary analyses of baseline assessments of two mind-body intervention studies for women with breast cancer undergoing radiotherapy in the USA (N = 62) and China (N = 97) are presented. All participants completed measures of depressive symptoms (CES-D) and cancer-specific QOL (FACT-B). We examined cultural differences on positive and negative affect items on the CES-D. Results Controlling for demographic factors, ANCOVA revealed a significant cultural difference in positive (F = 7.99, p = 0.005) but not negative affect (p = 0.82) with Chinese women reporting lower positive affect compared to US women (Chinese = 6.97 vs. US = 8.31). There was also a significant cultural difference (F = 3.94, p = 0.03) in the association between positive affect and QOL so that lower positive affect was more strongly associated with worse emotional well-being in Chinese (beta = 0.57, p < 0.0001) than US women (beta = 0.35, p < 0.01). Conclusions Chinese women reported lower positive affect compared to US women and lower levels of positive affect were more strongly associated with worse QOL. Special attention is needed when examining mental health in different cultures to ascertain effective delivery of clinical services to those in need. PMID:28233122

  6. Culture Wires the Brain: A Cognitive Neuroscience Perspective.

    PubMed

    Park, Denise C; Huang, Chih-Mao

    2010-07-01

    There is clear evidence that sustained experiences may affect both brain structure and function. Thus, it is quite reasonable to posit that sustained exposure to a set of cultural experiences and behavioral practices will affect neural structure and function. The burgeoning field of cultural psychology has often demonstrated the subtle differences in the way individuals process information-differences that appear to be a product of cultural experiences. We review evidence that the collectivistic and individualistic biases of East Asian and Western cultures, respectively, affect neural structure and function. We conclude that there is limited evidence that cultural experiences affect brain structure and considerably more evidence that neural function is affected by culture, particularly activations in ventral visual cortex-areas associated with perceptual processing. © The Author(s) 2010.

  7. Cross-Cultural Differences in the Processing of Non-Verbal Affective Vocalizations by Japanese and Canadian Listeners

    PubMed Central

    Koeda, Michihiko; Belin, Pascal; Hama, Tomoko; Masuda, Tadashi; Matsuura, Masato; Okubo, Yoshiro

    2013-01-01

    The Montreal Affective Voices (MAVs) consist of a database of non-verbal affect bursts portrayed by Canadian actors, and high recognitions accuracies were observed in Canadian listeners. Whether listeners from other cultures would be as accurate is unclear. We tested for cross-cultural differences in perception of the MAVs: Japanese listeners were asked to rate the MAVs on several affective dimensions and ratings were compared to those obtained by Canadian listeners. Significant Group × Emotion interactions were observed for ratings of Intensity, Valence, and Arousal. Whereas Intensity and Valence ratings did not differ across cultural groups for sad and happy vocalizations, they were significantly less intense and less negative in Japanese listeners for angry, disgusted, and fearful vocalizations. Similarly, pleased vocalizations were rated as less intense and less positive by Japanese listeners. These results demonstrate important cross-cultural differences in affective perception not just of non-verbal vocalizations expressing positive affect (Sauter et al., 2010), but also of vocalizations expressing basic negative emotions. PMID:23516137

  8. Cross-cultural differences in the processing of non-verbal affective vocalizations by Japanese and canadian listeners.

    PubMed

    Koeda, Michihiko; Belin, Pascal; Hama, Tomoko; Masuda, Tadashi; Matsuura, Masato; Okubo, Yoshiro

    2013-01-01

    The Montreal Affective Voices (MAVs) consist of a database of non-verbal affect bursts portrayed by Canadian actors, and high recognitions accuracies were observed in Canadian listeners. Whether listeners from other cultures would be as accurate is unclear. We tested for cross-cultural differences in perception of the MAVs: Japanese listeners were asked to rate the MAVs on several affective dimensions and ratings were compared to those obtained by Canadian listeners. Significant Group × Emotion interactions were observed for ratings of Intensity, Valence, and Arousal. Whereas Intensity and Valence ratings did not differ across cultural groups for sad and happy vocalizations, they were significantly less intense and less negative in Japanese listeners for angry, disgusted, and fearful vocalizations. Similarly, pleased vocalizations were rated as less intense and less positive by Japanese listeners. These results demonstrate important cross-cultural differences in affective perception not just of non-verbal vocalizations expressing positive affect (Sauter et al., 2010), but also of vocalizations expressing basic negative emotions.

  9. Can cultural differences lead to accidents? Team cultural differences and sociotechnical system operations.

    PubMed

    Strauch, Barry

    2010-04-01

    I discuss cultural factors and how they may influence sociotechnical system operations. Investigations of several major transportation accidents suggest that cultural factors may have played a role in the causes of the accidents. However, research has not fully addressed how cultural factors can influence sociotechnical systems. I review literature on cultural differences in general and cultural factors in sociotechnical systems and discuss how these differences can affect team performance in sociotechnical systems. Cultural differences have been observed in social and interpersonal dimensions and in cognitive and perceptual styles; these differences can affect multioperator team performance. Cultural factors may account for team errors in sociotechnical systems, most likely during high-workload, high-stress operational phases. However, much of the research on cultural factors has methodological and interpretive shortcomings that limit their applicability to sociotechnical systems. Although some research has been conducted on the role of cultural differences on team performance in sociotechnical system operations, considerable work remains to be done before the effects of these differences can be fully understood. I propose a model that illustrates how culture can interact with sociotechnical system operations and suggest avenues of future research. Given methodological challenges in measuring cultural differences and team performance in sociotechnical system operations, research in these systems should use a variety of methodologies to better understand how culture can affect multioperator team performance in these systems.

  10. How Cultural Differences Affect Written and Oral Communication: The Case of Peer Response Groups.

    ERIC Educational Resources Information Center

    Nelson, Gayle L.

    1997-01-01

    Peer response groups contribute to students' effectiveness as writers in any field, but cultural differences in communication affect interactions within the group. Culture-based dimensions on which communication may differ include individualism/collectivism, power distance, concept of "face," and communication style. Recommendations are…

  11. From putative genes to temperament and culture: cultural characteristics of the distribution of dominant affective temperaments in national studies.

    PubMed

    Gonda, Xenia; Vázquez, Gustavo H; Akiskal, Kareen K; Akiskal, Hagop S

    2011-06-01

    Affective temperaments may carry distinct evolutionary advantages both on the individual or a group level, so we can expect that in different cultural and national samples the frequency of dominant affective temperaments will show characteristic differences. The aim of the present study was to investigate the characteristics of distribution of dominant affective temperaments in different national studies of general non-clinical population. In our study we included six studies published in different countries around the world (Argentina, Germany, Hungary, Korea, Portugal, and Lebanon) which investigated a large sample of non-clinical population using TEMPS-A, and reported frequencies for dominant affective temperaments. The frequencies of dominant affective temperaments were compared using chi square tests. We found a significant difference in the frequency of affective temperaments among the different national studies in case of the cyclothymic, hyperthymic and irritable temperaments. We found important parallels between the frequency of affective temperaments and cultural dimensions described by Hofstede (Hofstede and Hofstede, 2005). The characteristics encompassed by the depressive temperament show considerable similarities with Hofstede's individualism-collectivism dimension, while those of the hyperthymic temperament seem to be similar to uncertainty avoidance, and the irritable temperament shows similarities with Hofstede's power distance. Furthermore, the relative frequency of these dominant temperaments in case of the different national samples paralleled the relative scores of these countries on the corresponding cultural scales. Our results indicate an important relationship between affective temperaments and cultural dimensions, which suggests that these phenomena may be the manifestations of the same genetically determined predispositions in different forms. We included a study by Erfurth et al. (2005), in which affective temperaments were evaluated using the TEMPS-M. Also, in the Korean study the Korean version of the TEMPS-A was not validated. Copyright © 2010 Elsevier B.V. All rights reserved.

  12. Nencki Affective Picture System: Cross-Cultural Study in Europe and Iran

    PubMed Central

    Riegel, Monika; Moslehi, Abnoos; Michałowski, Jarosław M.; Żurawski, Łukasz; Horvat, Marko; Wypych, Marek; Jednoróg, Katarzyna; Marchewka, Artur

    2017-01-01

    Although emotions have been assumed conventionally to be universal, recent studies have suggested that various aspects of emotions may be mediated by cultural background. The purpose of our research was to test these contradictory views, in the case of the subjective evaluation of visual affective stimuli. We also sought to validate the recently introduced Nencki Affective Picture System (NAPS) database on a different cultural group. Since there has been, to date, no attempt to compare the emotions of a culturally distinct sample of Iranians with those of Europeans, subjective ratings were collected from 40 Iranians and 39 Europeans. Each cultural group was asked separately to provide normative affective ratings and classify pictures according to discrete emotions. The results were analyzed to identify cultural differences in the ratings of individual images. One hundred and seventy NAPS pictures were rated with regard to the intensity of the basic emotions (happiness, sadness, fear, surprise, anger, and disgust) they elicited, as well as in terms of affective dimensions (valence and arousal). Contrary to previous studies using the International Affective Picture System, our results for Europeans and Iranians show that neither the ratings for affective dimensions nor for basic emotions differed across cultural groups. In both cultural groups, the relationship between valence and arousal ratings could be best described by a classical boomerang-shaped function. However, the content of the pictures (animals, faces, landscapes, objects, or people) had a significant effect on the ratings for valence and arousal. These findings indicate that further studies in cross-cultural affective research should control for the content of stimuli. PMID:28316576

  13. Nencki Affective Picture System: Cross-Cultural Study in Europe and Iran.

    PubMed

    Riegel, Monika; Moslehi, Abnoos; Michałowski, Jarosław M; Żurawski, Łukasz; Horvat, Marko; Wypych, Marek; Jednoróg, Katarzyna; Marchewka, Artur

    2017-01-01

    Although emotions have been assumed conventionally to be universal, recent studies have suggested that various aspects of emotions may be mediated by cultural background. The purpose of our research was to test these contradictory views, in the case of the subjective evaluation of visual affective stimuli. We also sought to validate the recently introduced Nencki Affective Picture System (NAPS) database on a different cultural group. Since there has been, to date, no attempt to compare the emotions of a culturally distinct sample of Iranians with those of Europeans, subjective ratings were collected from 40 Iranians and 39 Europeans. Each cultural group was asked separately to provide normative affective ratings and classify pictures according to discrete emotions. The results were analyzed to identify cultural differences in the ratings of individual images. One hundred and seventy NAPS pictures were rated with regard to the intensity of the basic emotions (happiness, sadness, fear, surprise, anger, and disgust) they elicited, as well as in terms of affective dimensions (valence and arousal). Contrary to previous studies using the International Affective Picture System, our results for Europeans and Iranians show that neither the ratings for affective dimensions nor for basic emotions differed across cultural groups. In both cultural groups, the relationship between valence and arousal ratings could be best described by a classical boomerang-shaped function. However, the content of the pictures (animals, faces, landscapes, objects, or people) had a significant effect on the ratings for valence and arousal. These findings indicate that further studies in cross-cultural affective research should control for the content of stimuli.

  14. Cultures differ in the ability to enhance affective neural responses.

    PubMed

    Varnum, Michael E W; Hampton, Ryan S

    2017-10-01

    The present study (N = 55) used an event-related potential paradigm to investigate whether cultures differ in the ability to upregulate affective responses. Using stimuli selected from the International Affective Picture System, we found that European-Americans (N = 29) enhanced central-parietal late positive potential (LPP) (400-800 ms post-stimulus) responses to affective stimuli when instructed to do so, whereas East Asians (N = 26) did not. We observed cultural differences in the ability to enhance central-parietal LPP responses for both positively and negativelyvalenced stimuli, and the ability to enhance these two types of responses was positively correlated for Americans but negatively for East Asians. These results are consistent with the notion that cultural variations in norms and values regarding affective expression and experiences shape how the brain regulates emotions.

  15. Acculturative stressors and acculturative strategies as predictors of negative affect among Chinese international students in Australia and Hong Kong: a cross-cultural comparative study.

    PubMed

    Pan, Jia-Yan; Wong, Daniel Fu Keung

    2011-11-01

    There are few studies comparing cross-cultural adaptation of migrant groups in two different cultural settings. This study compares the level of negative affect and acculturative stressors between Chinese international students in Australia and Mainland Chinese students in Hong Kong. The predictive effects of acculturative stressors and acculturative strategies on negative affect were also compared between the two groups. A total of 606 graduate students were recruited for a cross-sectional survey in Melbourne, and Hong Kong, China. The measurement included the Acculturative Hassles Scale for Chinese Students, Acculturative Strategy Scale, and Chinese Affect Scale. Independent t-tests and hierarchical regression analysis were conducted for data analysis. Chinese international students in Australia were found to encounter more acculturative stressors and experience a higher level of negative affect than their counterparts in Hong Kong. The acculturative stressor of academic work and a marginalization strategy significantly predicted negative affect in both groups. The acculturative stressor of cultural difference predicted negative affect in the Hong Kong sample, and assimilation strategy predicted negative affect in the Australian sample only. It is more difficult for Chinese international students to adapt to a host society with greater cultural distance. Cross-cultural comparative study helps to find out culture-general and culture-specific predictors of acculturation and helps design tailor-made intervention programs for international students across cultures.

  16. Learning what feelings to desire: socialization of ideal affect through children's storybooks.

    PubMed

    Tsai, Jeanne L; Louie, Jennifer Y; Chen, Eva E; Uchida, Yukiko

    2007-01-01

    Previous findings suggest that cultural factors influence ideal affect (i.e., the affective states that people ideally want to feel). Three studies tested the hypothesis that cultural differences in ideal affect emerge early in life and are acquired through exposure to storybooks. In Study 1, the authors established that consistent with previous findings, European American preschoolers preferred excited (vs. calm) states more (indexed by activity and smile preferences) and perceived excited (vs. calm) states as happier than Taiwanese Chinese preschoolers. In Study 2, it was observed that similar differences were reflected in the pictures (activities, expressions, and smiles) of best-selling storybooks in the United States and Taiwan. Study 3 found that across cultures, exposure to exciting (vs. calm) storybooks altered children's preferences for excited (vs. calm) activities and their perceptions of happiness. These findings suggest that cultural differences in ideal affect may be due partly to differential exposure to calm and exciting storybooks.

  17. Young people's topography of musical functions: personal, social and cultural experiences with music across genders and six societies.

    PubMed

    Boer, Diana; Fischer, Ronald; Tekman, Hasan Gürkan; Abubakar, Amina; Njenga, Jane; Zenger, Markus

    2012-01-01

    How can we understand the uses of music in daily life? Music is a universal phenomenon but with significant interindividual and cultural variability. Listeners' gender and cultural background may influence how and why music is used in daily life. This paper reports the first investigation of a holistic framework and a new measure of music functions (RESPECT-music) across genders and six diverse cultural samples (students from Germany, Kenya, Mexico, New Zealand, Philippines, and Turkey). Two dimensions underlie the mental representation of music functions. First, music can be used for contemplation or affective functions. Second, music can serve intrapersonal, social, and sociocultural functions. Results reveal that gender differences occur for affective functions, indicating that female listeners use music more for affective functions, i.e., emotional expression, dancing, and cultural identity. Country differences are moderate for social functions (values, social bonding, dancing) and strongest for sociocultural function (cultural identity, family bonding, political attitudes). Cultural values, such as individualism-collectivism and secularism-traditionalism, can help explain cross-cultural differences in the uses of music. Listeners from more collectivistic cultures use music more frequently for expressing values and cultural identity. Listeners from more secular and individualistic cultures like to dance more. Listeners from more traditional cultures use music more for expressing values and cultural identity, and they bond more frequently with their families over music. The two dimensions of musical functions seem systematically underpinned by listeners' gender and cultural background. We discuss the uses of music as behavioral expressions of affective and contemplative as well as personal, social, and sociocultural aspects in terms of affect proneness and cultural values.

  18. Cultural orientation moderates the association between desired affect and depressed mood among Chinese international students living in the United States.

    PubMed

    Lin, Jiquan; Dmitrieva, Julia

    2018-04-26

    Culture is thought to shape an individual's ideal/desired emotions, which may in turn regulate actual emotional experiences (Tsai, Knutson, & Fung, 2006). In particular, European Americans tend to favor high-arousal positive (HAP) affect, whereas East Asians favor low-arousal positive affect. This study examined whether cultural adaptation from the East Asian to Western culture is associated with similar differences in ideal and actual affect. We recruited 150 Chinese international students enrolled in a midsize university in the United States and investigated the role of acculturation to U.S. culture in participants' ideal and actual affect as well as associated differences in depressive symptoms. Results showed that acculturation was associated with higher ideal and actual HAP affect (but not lower low-arousal positive affect). Consistent with Mauss et al. (2012), higher ideal HAP affect was directly associated with higher depressive symptoms for all participants. However, among participants with higher orientation to the U.S. culture, higher ideal HAP also had an indirect protective association with depressed mood (i.e., higher ideal HAP affect was associated with higher actual HAP affect, which in turn was associated with lower depressed mood). (PsycINFO Database Record (c) 2018 APA, all rights reserved).

  19. A cross-cultural survey of residents' perceived barriers in questioning/challenging authority.

    PubMed

    Kobayashi, H; Pian-Smith, M; Sato, M; Sawa, R; Takeshita, T; Raemer, D

    2006-08-01

    To identify perceived barriers to residents' questioning or challenging their seniors, to determine how these barriers affect decisions, and to assess how these barriers differ across cultures. A written questionnaire was administered to residents in teaching hospitals in the US and Japan to assess factors affecting residents' willingness to question or challenge their superiors. The responses were analyzed for statistical significance of differences between the two cultures and to determine the importance of issues affecting decisions. Questionnaires were completed by 175 US and 65 Japanese residents, with an overall response rate of 71%. Trainees from both countries believe that questioning and challenging contribute to safety. The perceived importance of specific beliefs about the workplace differed across cultures in seven out of 22 questions. Residents' decisions to make a challenge were related to the relationships and perceived response of the superiors. There was no statistical difference between the US and Japanese residents in terms of the threshold for challenging their seniors. We have identified attributes of residents' beliefs of communication, including several cross-cultural differences in the importance of values and issues affecting one's decision to question or challenge. In contrast, there was no difference in the threshold for challenging seniors by the Japanese and US residents studied. Changes in organizational and professional culture may be as important, if not more so, than national culture to encourage "speaking up". Residents should be encouraged to overcome barriers to challenging, and training programs should foster improved relationships and communication between trainers and trainees.

  20. Gelling agents and culture vessels affect in vitro multiplication of banana plantlets.

    PubMed

    Kaçar, Y A; Biçen, B; Varol, I; Mendi, Y Y; Serçe, S; Cetiner, S

    2010-03-09

    Agar is the most commonly used gelling agent in media for plant tissue culture. Because of the high price of tissue-culture-grade agar, attempts have been made to identify suitable alternatives. The type of culture vessel and lid also affects the gaseous composition inside the vessel as well as light penetration. In turn, the vessel affects growth parameters, such as shoot elongation, proliferation and fresh weight, as well as hyperhydric degradation processes. We examined the effects of different culture vessels, including commercial glass jars, magenta boxes, and disposable containers, as well as different gelling agents (agar-agar, Agargel, Phytagel, and plant agar) on the micropropagation of Dwarf Cavendish bananas in an effort to find a combination that yields large numbers of high-quality seedlings. The different culture vessels did not significantly affect seedling culture success. The medium significantly affected shoot weight. Phytagel resulted in the highest shoot weight (overall mean = 2.4 g), while agar, Agargel and plant agar resulted in 1.7, 2.2 and 2.2 g, respectively. Disposable container/Phytagel and Magenta/Agargel combinations yielded the highest shoot weights (2.9 and 3.0 g, respectively). Mean shoot length increased progressively with subculture (four subcultures were made). The highest mean shoot length was obtained with Phytagel and Agargel media (6.4 and 6.3 cm, respectively). Shoot number was significantly affected by medium only at subculture 4. Overall, the highest mean shoot length was obtained with the Magenta/Agargel combination (8.5 cm). Phytagel and plant agar gave higher mean shoot number than agar and Agargel (2.1, 2.1 and 1.7 and 1.9, respectively). The costs of the media and of the culture vessels need to be taken into account for final choice of the banana shoot culture system.

  1. Exploring the East-West Divide in Prevalence of Affective Disorder: A Case for Cultural Differences in Coping With Negative Emotion.

    PubMed

    De Vaus, June; Hornsey, Matthew J; Kuppens, Peter; Bastian, Brock

    2017-10-01

    Lifetime rates of clinical depression and anxiety in the West tend to be approximately 4 to 10 times greater than rates in Asia. In this review, we explore one possible reason for this cross-cultural difference, that Asian cultures think differently about emotion than do Western cultures and that these different systems of thought help explain why negative affect does not escalate into clinical disorder at the same rate. We review research from multiple disciplines-including cross-cultural psychology, social cognition, clinical psychology, and psychiatry-to make the case that the Eastern holistic principles of contradiction (each experience is associated with its opposite), change (the world exists in a state of constant flux), and context (the interconnectedness of all things) fundamentally shape people's experience of emotions in different cultures. We then review evidence for how these cultural differences influence how successfully people use common emotion regulation strategies such as rumination and suppression.

  2. Self-construal, affective valence of the encounter, and quality of social interactions: Within and cross-culture examination.

    PubMed

    Kafetsios, Konstantinos; Hess, Ursula; Nezlek, John B

    2018-01-01

    In two samples, one from Greece and another from Germany, we examined relationships between self-construal, emotional experience, and the quality of social interactions. In Greece, a more collectivistic culture, the negative affect people experienced in social interactions was more weakly related to the quality of social interactions for those higher in interdependent self-construal than it was for those lower in interdependent self-construal. In Germany, a more independent culture, a contrasting pattern was observed such that the positive affect people experienced in social interaction was more strongly related to the quality of social interactions for those higher in independent self-construal than it was for those lower in independent self-construal. These findings suggest that positive and negative affect in social encounters can have different effects for persons with independent and interdependent cultural orientations within different cultural settings.

  3. Culture medium composition affects the gene expression pattern and in vitro development potential of bovine somatic cell nuclear transfer (SCNT) embryos.

    PubMed

    Arias, María E; Ross, Pablo J; Felmer, Ricardo N

    2013-01-01

    Different culture systems have been studied that support development of somatic cell nuclear transfer (SCNT) embryos up to the blastocyst stage. However, the use of sequential and two-step culture systems has been less studied. The objective of the present study was to examine the developmental potential and quality of bovine SCNT embryos cultured in different two-step culture media based on KSOM, SOF and the macromolecules FBS and BSA (K-K/FBS, K-S/BSA and K-K/BSA, respectively). No differences were observed in the cleavage rate for any of the culture systems. However, there was a significant difference (P<0.01) in the rate of blastocyst development, with the K-K/ FBS culture system yielding a higher rate of blastocysts (28%) compared to other treatments (18 and 15%, for K-S/BSA and K-K/BSA, respectively). Although quality of embryos, as assessed by the total number of cells, was not different, the apoptosis index was significantly affected in the sequential culture system (K-S/BSA). Gene expression analysis showed alterations of DNMT1, IGF2, LIF, and PRDX6 genes in embryos cultured in K-S/FBS and of SOD2 in embryos cultured in K-K/BSA. In conclusion, we demonstrated that culture medium may affect not only the developmental potential of SCNT embryos but also, more importantly, the gene expression pattern and apoptotic index, presenting the possibility to manipulate the culture medium composition to modulate global gene expression and improve the overall efficiency of this technique.

  4. Cross-cultural aging in cognitive and affective components of subjective well-being.

    PubMed

    Pethtel, Olivia; Chen, Yiwei

    2010-09-01

    The present study examined age and cultural differences in cognitive and affective components of subjective well-being. A sample of 188 American and Chinese young and older adults completed surveys measuring self-life satisfaction, perceived family's life satisfaction, positive affect, and negative affect. Across cultures, older adults reported lower negative affect than did young adults. Americans reported higher self-life satisfaction, perceived family's life satisfaction, and positive affect than did Chinese. In addition, perceived family's life satisfaction was more related to self-life satisfaction for Chinese than for Americans. Findings are discussed in light of socioemotional selectivity theory and theories on culture and self-construal. (c) 2010 APA, all rights reserved.

  5. Cultural differences in sensitivity to social context: detecting affective incongruity using the N400.

    PubMed

    Goto, Sharon G; Yee, Alicia; Lowenberg, Kelly; Lewis, Richard S

    2013-01-01

    East Asians and Asian-Americans tend to allocate relatively greater attention to background context compared to European Americans across a variety of cognitive and neural measures. We sought to extend these findings of cultural differences to affective stimuli using the N400, which has been shown to be sensitive to deep processing of affective information. The degree to which Asian-Americans and European Americans responded to semantic incongruity between emotionally expressive faces (i.e., smiling or frowning) and background affective scenes was measured. As predicted, Asian-Americans showed a greater N400 to incongruent trials than to congruent trials. In contrast, European Americans showed no difference in amplitude across the two conditions. Furthermore, greater affective N400 incongruity was associated with higher interdependent self-construals. These data suggest that Asian-Americans and those with interdependent self-construals process the relationship between perceived facial emotion and affective background context to a greater degree than European Americans and those with independent self-construals. Implications for neural and cognitive differences in everyday social interactions, and cultural differences in analytic and holistic thinking are discussed.

  6. Culture and social support: neural bases and biological impact.

    PubMed

    Sherman, David K; Kim, Heejung S; Taylor, Shelley E

    2009-01-01

    Social support is an effective means by which people cope with stressful events, and consequently, it beneficially affects health and well-being. Yet there are profound cultural differences in the effectiveness of different types of support and how people use their support networks. In this paper, we examine research on the impact of culture on social support, the neural underpinnings of social support, and how cultural differences in social support seeking are manifested biologically. We focus on cultural factors that may affect individuals' decisions to seek or not to seek social support and how culture moderates the impact of support seeking on biological and psychological health outcomes. We also examine recent research on the interaction between genes and culture in social support use. Discussion centers on the importance of developing an overarching framework of social support that integrates health psychology, cultural psychology, social neuroscience, and genetics.

  7. Psychosocial and cultural factors affecting the perceived risk of genetically modified food: an overview of the literature.

    PubMed

    Finucane, Melissa L; Holup, Joan L

    2005-04-01

    The rapid globalization of the world economy has increased the need for an astute understanding of cultural differences in perceptions, values, and ways of thinking about new food technologies. In this paper, we describe how socio-psychological and cultural factors may affect public perceptions of the risk of genetically modified (GM) food. We present psychological, sociological, and anthropological research on risk perception as a framework for understanding cross-national differences in reactions to GM food. Differences in the cultural values and circumstances of people in the US, European countries, and the developing world are examined. The implications of cultural theory for risk communication and decision making about GM food are discussed and directions for future research highlighted.

  8. How European American and Taiwanese mothers talk to their children about learning.

    PubMed

    Li, Jin; Fung, Heidi; Bakeman, Roger; Rae, Katharine; Wei, Wanchun

    2014-01-01

    Little cross-cultural research exists on parental socialization of children's learning beliefs. The current study compared 218 conversations between European American and Taiwanese mothers and children (6-10 years) about good and poor learning. The findings support well-documented cultural differences in learning beliefs. European Americans mentioned mental activities and positive affect more, whereas Taiwanese mentioned learning virtues and negative affect more. Mothers, especially European American, reciprocated their children's talk about mental activities, learning virtues, and negative affect. Children, especially Taiwanese, reciprocated their mother's talk about positive affect. Mothers invoked more mental activities and positive affect when discussing good learning, but more learning virtues and negative affect when discussing poor learning. These findings reveal a source of cultural differences in beliefs and potential enculturation. © 2013 The Authors. Child Development © 2013 Society for Research in Child Development, Inc.

  9. Feelings about culture scales: development, factor structure, reliability, and validity.

    PubMed

    Maffini, Cara S; Wong, Y Joel

    2015-04-01

    Although measures of cultural identity, values, and behavior exist in the multicultural psychological literature, there is currently no measure that explicitly assesses ethnic minority individuals' positive and negative affect toward culture. Therefore, we developed 2 new measures called the Feelings About Culture Scale--Ethnic Culture and Feelings About Culture Scale--Mainstream American Culture and tested their psychometric properties. In 6 studies, we piloted the measures, conducted factor analyses to clarify their factor structure, and examined reliability and validity. The factor structure revealed 2 dimensions reflecting positive and negative affect for each measure. Results provided evidence for convergent, discriminant, criterion-related, and incremental validity as well as the reliability of the scales. The Feelings About Culture Scales are the first known measures to examine both positive and negative affect toward an individual's ethnic culture and mainstream American culture. The focus on affect captures dimensions of psychological experiences that differ from cognitive and behavioral constructs often used to measure cultural orientation. These measures can serve as a valuable contribution to both research and counseling by providing insight into the nuanced affective experiences ethnic minority individuals have toward culture. (c) 2015 APA, all rights reserved).

  10. An fMRI investigation of the effects of culture on evaluations of stigmatized individuals.

    PubMed

    Krendl, Anne C

    2016-01-01

    Certain groups (e.g., women, older adults, and the economically disadvantaged) are universally stigmatized. Numerous studies, however, have identified cross-cultural differences in the attitudes expressed toward stigmatized groups. These differences may potentially be due to existing cross-cultural dissimilarities in social status for some groups. The current study used fMRI to examine whether Chinese and Caucasian-American participants engage the same cognitive and affective mechanisms when perceiving stigmatized individuals with similarly low social status in both cultures (homeless individuals), but different cognitive and/or affective processes when evaluating stigmatized individuals whose status differs across cultures (older adults). Using a social neuroscience approach can provide unique insight into this question because the neural regions involved in cognitive and affective evaluations of stigmatized individuals have been well characterized. Results revealed that Chinese participants and Caucasian-American participants engaged similar patterns of negative affective processing associated with disgust (left anterior insula) when evaluating homeless individuals. Moreover, self-reported negative explicit attitudes toward homeless individuals were associated with increased activity in the insula. However, Chinese participants and Caucasian-American participants engaged increased activity in neural regions associated with status (ventral striatum) when they evaluated older adults. Moreover, self-reported attitudes toward older adults and ventral striatal activity were correlated with the extent to which participants reported being affiliated with their respective cultural traditions. Copyright © 2015 Elsevier Inc. All rights reserved.

  11. How does context affect assessments of facial emotion? The role of culture and age

    PubMed Central

    Ko, Seon-Gyu; Lee, Tae-Ho; Yoon, Hyea-Young; Kwon, Jung-Hye; Mather, Mara

    2010-01-01

    People from Asian cultures are more influenced by context in their visual processing than people from Western cultures. In this study, we examined how these cultural differences in context processing affect how people interpret facial emotions. We found that younger Koreans were more influenced than younger Americans by emotional background pictures when rating the emotion of a central face, especially those younger Koreans with low self-rated stress. In contrast, among older adults, neither Koreans nor Americans showed significant influences of context in their face emotion ratings. These findings suggest that cultural differences in reliance on context to interpret others' emotions depend on perceptual integration processes that decline with age, leading to fewer cultural differences in perception among older adults than among younger adults. Furthermore, when asked to recall the background pictures, younger participants recalled more negative pictures than positive pictures, whereas older participants recalled similar numbers of positive and negative pictures. These age differences in the valence of memory were consistent across culture. PMID:21038967

  12. Effects of Lactobacillus plantarum Strain OLL2712 Culture Conditions on the Anti-inflammatory Activities for Murine Immune Cells and Obese and Type 2 Diabetic Mice.

    PubMed

    Toshimitsu, T; Ozaki, S; Mochizuki, J; Furuichi, K; Asami, Y

    2017-04-01

    Studies on the health-promoting effects of lactic acid bacteria (LAB) are numerous, but few provide examples of the relationship between LAB function and culture conditions. We verified the effect of differences in culture conditions on Lactobacillus plantarum OLL2712 functionality; this strain exhibits anti-inflammatory activity and preventive effects against metabolic disorders. We measured interleukin-10 (IL-10) and IL-12 production in murine immune cells treated with OLL2712 cells prepared under various culture conditions. The results showed that the IL-10-inducing activities of OLL2712 cells on murine immune cells differed dramatically between OLL2712 groups at different culture phases and using different culture medium components, temperatures, and neutralizing pHs. In particular, exponential-phase cells had much more IL-10-inducing activity than stationary-phase cells. We confirmed that the Toll-like receptor 2 (TLR2) stimulation activity of OLL2712 cells depended on culture conditions in conjunction with IL-10-inducing activity. We also demonstrated functional differences by culture phases in vivo ; OLL2712 cells at exponential phase had more anti-inflammatory activity and anti-metabolic-disorder effects on obese and diabetic mice than those by their stationary-phase counterparts. These results suggest that culture conditions affect the functionality of anti-inflammatory LAB. IMPORTANCE While previous studies demonstrated that culture conditions affected the immunomodulatory properties of lactic acid bacteria (LAB), few have comprehensively investigated the relationship between culture conditions and LAB functionality. In this study, we demonstrated several culture conditions of Lactobacillus plantarum OLL2712 for higher anti-inflammatory activity. We also showed that culture conditions concretely influenced the health-promoting functions of OLL2712 in vivo , particularly against metabolic disorders. Further, we characterized a novel mechanism by which changing LAB culture conditions affected immunomodulatory properties. Our results suggest that culture condition optimization is important for the production of LAB with anti-inflammatory activity. Copyright © 2017 American Society for Microbiology.

  13. Culture moderates changes in linguistic self-presentation and detail provision when deceiving others

    PubMed Central

    Larner, Samuel; Conchie, Stacey M.; Menacere, Tarek

    2017-01-01

    Change in our language when deceiving is attributable to differences in the affective and cognitive experience of lying compared to truth telling, yet these experiences are also subject to substantial individual differences. On the basis of previous evidence of cultural differences in self-construal and remembering, we predicted and found evidence for cultural differences in the extent to which truths and lies contained self (versus other) references and perceptual (versus social) details. Participants (N = 320) of Black African, South Asian, White European and White British ethnicity completed a catch-the-liar task in which they provided genuine and fabricated statements about either their past experiences or an opinion and counter-opinion. Across the four groups we observed a trend for using more/fewer first-person pronouns and fewer/more third-person pronouns when lying, and a trend for including more/fewer perceptual details and fewer/more social details when lying. Contrary to predicted cultural differences in emotion expression, all participants showed more positive affect and less negative affect when lying. Our findings show that liars deceive in ways that are congruent with their cultural values and norms, and that this may result in opposing changes in behaviour. PMID:28680668

  14. Cultural differences in moral judgment and behavior, across and within societies.

    PubMed

    Graham, Jesse; Meindl, Peter; Beall, Erica; Johnson, Kate M; Zhang, Li

    2016-04-01

    We review contemporary work on cultural factors affecting moral judgments and values, and those affecting moral behaviors. In both cases, we highlight examples of within-societal cultural differences in morality, to show that these can be as substantial and important as cross-societal differences. Whether between or within nations and societies, cultures vary substantially in their promotion and transmission of a multitude of moral judgments and behaviors. Cultural factors contributing to this variation include religion, social ecology (weather, crop conditions, population density, pathogen prevalence, residential mobility), and regulatory social institutions such as kinship structures and economic markets. This variability raises questions for normative theories of morality, but also holds promise for future descriptive work on moral thought and behavior. Copyright © 2015 Elsevier Ltd. All rights reserved.

  15. Developing Cultural Differences in Face Processing

    ERIC Educational Resources Information Center

    Kelly, David J.; Liu, Shaoying; Rodger, Helen; Miellet, Sebastien; Ge, Liezhong; Caldara, Roberto

    2011-01-01

    Perception and eye movements are affected by culture. Adults from Eastern societies (e.g. China) display a disposition to process information "holistically," whereas individuals from Western societies (e.g. Britain) process information "analytically." Recently, this pattern of cultural differences has been extended to face…

  16. Cross-Cultural Communication Patterns in Computer Mediated Communication

    ERIC Educational Resources Information Center

    Panina, Daria; Kroumova, Maya

    2015-01-01

    There are important cultural differences in attitudes towards and use of electronic text communication. Consistent with Hall's high-context/low-context conceptualization of culture, electronic inter-cultural communication, just as verbal inter-cultural communication, is affected by the culturally-specific assumptions and preferences of message…

  17. Relevant principal factors affecting the reproducibility of insect primary culture.

    PubMed

    Ogata, Norichika; Iwabuchi, Kikuo

    2017-06-01

    The primary culture of insect cells often suffers from problems with poor reproducibility in the quality of the final cell preparations. The cellular composition of the explants (cell number and cell types), surgical methods (surgical duration and surgical isolation), and physiological and genetic differences between donors may be critical factors affecting the reproducibility of culture. However, little is known about where biological variation (interindividual differences between donors) ends and technical variation (variance in replication of culture conditions) begins. In this study, we cultured larval fat bodies from the Japanese rhinoceros beetle, Allomyrina dichotoma, and evaluated, using linear mixed models, the effect of interindividual variation between donors on the reproducibility of the culture. We also performed transcriptome analysis of the hemocyte-like cells mainly seen in the cultures using RNA sequencing and ultrastructural analyses of hemocytes using a transmission electron microscope, revealing that the cultured cells have many characteristics of insect hemocytes.

  18. Cultural differences among health professionals: a case illustration.

    PubMed

    Perkins, H S; Supik, J D; Hazuda, H P

    1998-01-01

    This study illustrates that cultural differences arise among similarly trained health professionals. Health professionals must learn to communicate sensitively with colleagues from other cultures, to respect their values, and to recognize and resolve cultural differences that affect patient care. In this shrinking, multicultural world, health professionals cannot afford the comfortable illusion that all similarly trained practitioners share the same values about the care of patients and professional conduct.

  19. How does context affect assessments of facial emotion? The role of culture and age.

    PubMed

    Ko, Seon-Gyu; Lee, Tae-Ho; Yoon, Hyea-Young; Kwon, Jung-Hye; Mather, Mara

    2011-03-01

    People from Asian cultures are more influenced by context in their visual processing than people from Western cultures. In this study, we examined how these cultural differences in context processing affect how people interpret facial emotions. We found that younger Koreans were more influenced than younger Americans by emotional background pictures when rating the emotion of a central face, especially those younger Koreans with low self-rated stress. In contrast, among older adults, neither Koreans nor Americans showed significant influences of context in their face emotion ratings. These findings suggest that cultural differences in reliance on context to interpret others' emotions depend on perceptual integration processes that decline with age, leading to fewer cultural differences in perception among older adults than among younger adults. Furthermore, when asked to recall the background pictures, younger participants recalled more negative pictures than positive pictures, whereas older participants recalled similar numbers of positive and negative pictures. These age differences in the valence of memory were consistent across culture. (c) 2011 APA, all rights reserved.

  20. Cultural factors affecting the differential performance of Israeli and Palestinian children on the Loewenstein Occupational Therapy Cognitive Assessment.

    PubMed

    Josman, Naomi; Abdallah, Taisir M; Engel-Yeger, Batya

    2010-01-01

    Cognitive performance is essential for children's functioning and may also predict school readiness. The suitability of Western standardized assessments for cognitive performance among children from different cultures needs to be elaborated. This study referred to the existence of differences in cognitive performance between and within children from the middle-east-Israeli and Palestinian on the Loewenstein Occupational Therapy Cognitive Assessment (LOTCA), by elucidating cultural effects on the construct validity of the LOTCA using factor analysis. Participants included 101 Israeli and 125 Palestinian children from kindergarten, first and second grade who underwent the LOTCA. Factor analysis revealed four factors underlying items on the LOTCA, explaining the differences found between Israeli and Palestinian children in most of LOTCA subtests. Culture may affect the construct validity of the LOTCA and may explain the difference in performance between both cultural groups. LOTCA's validity as well as the validity of other instruments on which norms and decisions regarding the child's development and performance are made should be further evaluated among children from different cultural backgrounds. 2010 Elsevier Ltd. All rights reserved.

  1. Early Social Cognition in Three Cultural Contexts

    ERIC Educational Resources Information Center

    Callaghan, Tara; Moll, Henrike; Rakoczy, Hannes; Warneken, Felix; Liszkowski, Ulf; Behne, Tanya; Tomasello, Michael

    2011-01-01

    The influence of culture on cognitive development is well established for school age and older children. But almost nothing is known about how different parenting and socialization practices in different cultures affect infants' and young children's earliest emerging cognitive and social-cognitive skills. In the current monograph, we report a…

  2. Perspective Taking, Cultural Stress, and the Individual: From the Inside Out

    DTIC Science & Technology

    2014-05-01

    how Soldier individual differences, cultural stress, and perspective taking affect decision making through the Relevant Information for Social ...Cultural Depiction. This report will show that inclusion of individual difference variables is essential to social -cultural model development, which will...are capable of inducing a stress response. Common external stressors fall within four general categories: personal, social /familial, work, and the

  3. Acculturative Stressors and Acculturative Strategies as Predictors of Negative Affect among Chinese International Students in Australia and Hong Kong: A Cross-Cultural Comparative Study

    ERIC Educational Resources Information Center

    Pan, Jia-Yan; Wong, Daniel Fu Keung

    2011-01-01

    Objective: There are few studies comparing cross-cultural adaptation of migrant groups in two different cultural settings. This study compares the level of negative affect and acculturative stressors between Chinese international students in Australia and Mainland Chinese students in Hong Kong. The predictive effects of acculturative stressors and…

  4. Universal and culture-specific factors in the recognition and performance of musical affect expressions.

    PubMed

    Laukka, Petri; Eerola, Tuomas; Thingujam, Nutankumar S; Yamasaki, Teruo; Beller, Grégory

    2013-06-01

    We present a cross-cultural study on the performance and perception of affective expression in music. Professional bowed-string musicians from different musical traditions (Swedish folk music, Hindustani classical music, Japanese traditional music, and Western classical music) were instructed to perform short pieces of music to convey 11 emotions and related states to listeners. All musical stimuli were judged by Swedish, Indian, and Japanese participants in a balanced design, and a variety of acoustic and musical cues were extracted. Results first showed that the musicians' expressive intentions could be recognized with accuracy above chance both within and across musical cultures, but communication was, in general, more accurate for culturally familiar versus unfamiliar music, and for basic emotions versus nonbasic affective states. We further used a lens-model approach to describe the relations between the strategies that musicians use to convey various expressions and listeners' perceptions of the affective content of the music. Many acoustic and musical cues were similarly correlated with both the musicians' expressive intentions and the listeners' affective judgments across musical cultures, but the match between musicians' and listeners' uses of cues was better in within-cultural versus cross-cultural conditions. We conclude that affective expression in music may depend on a combination of universal and culture-specific factors.

  5. Effects of Cultural Assimilation in a Cross-Border M&A

    NASA Astrophysics Data System (ADS)

    Ito, Satoshi; Tamiya, Toshihiko; Fujimura, Shuzo

    2010-06-01

    This paper examines a merger of two companies with respect to the assimilation of corporate culture differences, particularly quality culture. The study aims to clarify the root cause of declines in quality ratings from customers after a merger despite maintaining products and services as they were before the merger, and whether cultural differences affect decision-making in a newly merged company. Examination is reported of the relationship between actions taken to assimilate quality culture and the resulting quality ratings given by customers, and analyzed is the time required to address quality incidents and the progress of cultural assimilation. A total of 301 incidents in the 5 years after a merger were analyzed, focusing on the time required to resolve the incidents. These empirical analyses reveal that the extent of cultural assimilation is associated with the speed of organizational decision-making and affects the quality ratings given by customers.

  6. Cross-cultural Temperamental Differences in Infants, Children, and Adults in the United States of America and Finland

    PubMed Central

    Gaias, Larissa M.; Gartstein, Maria A.; Fisher, Philip A.; Putnam, Samuel P.; Räikkönen, Katri; Komsi, Niina

    2012-01-01

    Cross-cultural differences in temperament were investigated between infants (n = 131, 84 Finns), children (n = 653, 427 Finns), and adults (n = 759, 538 Finns) from the United States of America and Finland. Participants from both cultures completed the Infant Behavior Questionnaire, Childhood Behavior Questionnaire, and the Adult Temperament Questionnaire. Across all ages, Americans received higher ratings on temperamental fearfulness than Finnish individuals, and also demonstrated higher levels of other negative affects at several time points. During infancy and adulthood, Finns tended to score higher on positive affect and elements of temperamental effortful control. Gender differences consistent with prior studies emerged cross-culturally, and were found to be more pronounced in the U.S. during childhood and in Finland during adulthood. PMID:22428997

  7. Focusing on the negative: cultural differences in expressions of sympathy.

    PubMed

    Koopmann-Holm, Birgit; Tsai, Jeanne L

    2014-12-01

    Feeling concern about the suffering of others is considered a basic human response, and yet we know surprisingly little about the cultural factors that shape how people respond to the suffering of another person. To this end, we conducted 4 studies that tested the hypothesis that American expressions of sympathy focus on the negative less and positive more than German expressions of sympathy, in part because Americans want to avoid negative states more than Germans do. In Study 1, we demonstrate that American sympathy cards contain less negative and more positive content than German sympathy cards. In Study 2, we show that European Americans want to avoid negative states more than Germans do. In Study 3, we demonstrate that these cultural differences in "avoided negative affect" mediate cultural differences in how comfortable Americans and Germans feel focusing on the negative (vs. positive) when expressing sympathy for the hypothetical death of an acquaintance's father. To examine whether greater avoided negative affect results in lesser focus on the negative and greater focus on the positive when responding to another person's suffering, in Study 4, American and German participants were randomly assigned to 1 of 2 conditions: (a) to "push negative images away" (i.e., increasing desire to avoid negative affect) from or (b) to "pull negative images closer" (i.e., decreasing desire to avoid negative affect) to themselves. Participants were then asked to pick a card to send to an acquaintance whose father had hypothetically just died. Across cultures, participants in the "push negative away" condition were less likely to choose sympathy cards with negative (vs. positive) content than were those in the "pull negative closer" condition. Together, these studies suggest that cultures differ in their desire to avoid negative affect and that these differences influence the degree to which expressions of sympathy focus on the negative (vs. positive). We discuss the implications of these findings for current models of sympathy, compassion, and helping.

  8. Making a Case for a Cross-Cultural Approach to Literacy in Appalachia.

    ERIC Educational Resources Information Center

    Thaller, Eva A.

    An insider/outsider approach to Appalachian culture examines cultural differences of Appalachian people that necessitate cross-cultural educational studies usually reserved for ethnic and racial minority groups. An overview focuses on current economic conditions affecting the region's culture and some stereotypes of Appalachians that remain…

  9. The Cultural Discontinuity Hypothesis: An Appalachian American Perspective in Eastern Kentucky

    ERIC Educational Resources Information Center

    Conner, Timothy W., II

    2017-01-01

    K.M. Tyler et al. (2008) propose a quantitative method to measure differences between school and home experiences had by students of ethnic minority status and how such differences (cultural discontinuity) may affect psychological factors related to student achievement. Although study of cultural discontinuity has been applied to understanding…

  10. A Comparative Study of Value Orientations of Chinese and American Families: A Communication View.

    ERIC Educational Resources Information Center

    Chen, Guo-Ming

    Because an understanding of the cultural value orientation leads to more effective communication with people from different cultures, this paper examines how the difference of cultural value orientations affects the communication process. The paper explores the dissimilarities of value orientations between Chinese and American families from the…

  11. Affective design identification on the development of batik convection product

    NASA Astrophysics Data System (ADS)

    Prastawa, H.; Purwaningsih, R.

    2017-11-01

    The affective design is increasingly applied to product development in order to meet the desires and preferences of customers. Batik is a traditional Indonesian culture containing historical and cultural values. The development of batik design is one of the efforts to strengthen the identity and superiority of Indonesia’s creative industries as well as to preserve batik as the cultural heritage of the nation. Batik product designs offered by the manufacturers do not necessarily correspond with the wishes of consumers, especially the affective values involved. Therefore it is necessary to identify consumer perceptions of convection- based batik product in the form of clothing and fabrics, especially the affective value as the consideration for the designer or manufacturer to develop design alternatives to batik convection products. This research aims to obtain information on consumer affective value, to identify the affective value perception differences among X and Y Generation and to classify affective value in the corresponding cluster of the batik products convection. This study uses Kansei engineering to determine the perception of affective design in the form of Kansei word. Cluster Analysis was used to form clusters that classify affective value of the same class. The results showed that there were 16 pairs of Kansei word which was worth as an affective consumer desire, the 3 indicators that had significant differences among X and Y Generation and 4 clusters with different characteristics.

  12. Explaining Differences in Subjective Well-Being Across 33 Nations Using Multilevel Models: Universal Personality, Cultural Relativity, and National Income.

    PubMed

    Cheng, Cecilia; Cheung, Mike W-L; Montasem, Alex

    2016-02-01

    This multinational study simultaneously tested three prominent hypotheses--universal disposition, cultural relativity, and livability--that explained differences in subjective well-being across nations. We performed multilevel structural equation modeling to examine the hypothesized relationships at both individual and cultural levels in 33 nations. Participants were 6,753 university students (2,215 men; 4,403 women; 135 did not specify), and the average age of the entire sample was 20.97 years (SD = 2.39). Both individual- and cultural-level analyses supported the universal disposition and cultural relativity hypotheses by revealing significant associations of subjective well-being with Extraversion, Neuroticism, and independent self-construal. In addition, interdependent self-construal was positively related to life satisfaction at the individual level only, whereas aggregated negative affect was positively linked with aggregate levels of Extraversion and interdependent self-construal at the cultural level only. Consistent with the livability hypothesis, gross national income (GNI) was related to aggregate levels of negative affect and life satisfaction. There was also a quadratic relationship between GNI and aggregated positive affect. Our findings reveal that universal disposition, cultural self-construal, and national income can elucidate differences in subjective well-being, but the multilevel analyses advance the literature by yielding new findings that cannot be identified in studies using individual-level analyses alone. © 2014 Wiley Periodicals, Inc.

  13. Cultural differences in medical communication: a review of the literature.

    PubMed

    Schouten, Barbara C; Meeuwesen, Ludwien

    2006-12-01

    Culture and ethnicity have often been cited as barriers in establishing an effective and satisfying doctor-patient relationship. The aim of this paper is to gain more insight in intercultural medical communication difficulties by reviewing observational studies on intercultural doctor-patient communication. In addition, a research model for studying this topic in future research is proposed. A literature review using online databases (Pubmed, Psychlit) was performed. Findings reveal major differences in doctor-patient communication as a consequence of patients' ethnic backgrounds. Doctors behave less affectively when interacting with ethnic minority patients compared to White patients. Ethnic minority patients themselves are also less verbally expressive; they seem to be less assertive and affective during the medical encounter than White patients. Most reviewed studies did not relate communication behaviour to possible antecedent culture-related variables, nor did they assess the effect of cultural variations in doctor-patient communication on outcomes, leaving us in the dark about reasons for and consequences of differences in intercultural medical communication. Five key predictors of culture-related communication problems are identified in the literature: (1) cultural differences in explanatory models of health and illness; (2) differences in cultural values; (3) cultural differences in patients' preferences for doctor-patient relationships; (4) racism/perceptual biases; (5) linguistic barriers. It is concluded that by incorporating these variables into a research model future research on this topic can be enhanced, both from a theoretical and a methodological perspective. Using a cultural sensitive approach in medical communication is recommended.

  14. Variability in biochemical components of the mussel (Mytilus galloprovincialis) cultured after Prestige oil spill.

    PubMed

    Peteiro, Laura G; Labarta, Uxío; Fernández-Reiriz, María José

    2007-05-01

    The biochemical composition (proteins, carbohydrates, glycogen, total lipids and lipid classes) of the mussel Mytilus galloprovincialis was investigated during an experimental culture using mussel seed from areas with different degree of exposure to the Prestige oil spill. The aim of the study was to identify alterations in the biochemical composition of mussel seed from natural populations commonly used in Galicia for mussel raft culture that might be linked to previous oil exposure. We have selected three mussel seed populations from Pindo, Miranda and Redes, that were characterised in a previous study according to the oil exposure three months after the spill. These populations were transplanted to a raft culture system in the Ría de Ares-Betanzos where our experimental culture followed standard commercial techniques from March 2003 to February 2004. Mussels from Pindo (characterised as the most affected area by the oil spill) showed marked differences in lipid composition with regard to other populations in the content of triacylglycerols, (P<0.001), free fatty acids (P<0.001) and phospholipids (P<0.05) at the onset of the culture. Although these differences in lipid composition might reflect their previous exposition to hydrocarbons, this pattern did not last longer most likely due to depuration of hydrocarbons stored in the tissues or by the development of certain tolerance to PAHs. These significant differences were not detected between Miranda (designed as hardly affected area) and Redes (designed as reference area) which may reflect that Miranda mussels were not affected or only hardly affected by the spill. With the exception of the onset of the culture, biochemical composition showed similar patterns in all mussel populations. Then, the fact of being cultured in a common environment seemed to be more responsible for the long-term variability in the energetic reserve than the origin of the populations or their previous biochemical status.

  15. Not all collectivisms are equal: opposing preferences for ideal affect between East Asians and Mexicans.

    PubMed

    Ruby, Matthew B; Falk, Carl F; Heine, Steven J; Villa, Covadonga; Silberstein, Orly

    2012-12-01

    Previous research has revealed differences in how people value and pursue positive affect in individualistic and collectivistic cultural contexts. Whereas Euro-Americans place greater value on high activation positive affect (HAP; e.g., excitement, enthusiasm, elation) than do Asian Americans and Hong Kong Chinese, the opposite is true for low activation positive affect (LAP; e.g., calmness, serenity, tranquility). Although the form of collectivism present in East Asia dictates that individuals control and subdue their emotional expressions so as to maintain harmonious relationships, the opposite norm emerges in Mexico and other Latin American countries, in that the cultural script of simpatía promotes harmony through the open and vibrant expression of positive emotion. Across two studies, we found that Mexicans display a pattern of HAP/LAP preference different from those from East Asian collectivistic cultures, endorsing HAP over LAP. PsycINFO Database Record (c) 2012 APA, all rights reserved.

  16. Sociocultural Context for Sex Differences in Addiction

    PubMed Central

    Becker, Jill B.; McClellan, Michelle; Reed, Beth Glover

    2017-01-01

    In this review we discuss the importance of investigating both sex and gender differences in addiction and relapse in studies of humans and in animal models. Addiction is both a cultural and biological phenomenon Sex and gender differences are not solely determined by our biology, nor are they entirely cultural; there are interactions between biology and the environment that are continuously played out throughout development. Lessons from the historical record illustrate how context and attitudes affect the way that substance use in men and women is regarded. Finally, cultural and environmental influences may differentially affect men and women, and affect how they respond to drugs of abuse and to treatment protocols. We recommend that both animal models and clinical research need to be developed to consider how contextual and social factors may influence the biological processes of addiction and relapse differentially in males and females. PMID:26935336

  17. Cross-cultural temperamental differences in infants, children, and adults in the United States of America and Finland.

    PubMed

    Gaias, Larissa M; Räikkönen, Katri; Komsi, Niina; Gartstein, Maria A; Fisher, Philip A; Putnam, Samuel P

    2012-04-01

    Cross-cultural differences in temperament were investigated between infants (n = 131, 84 Finns), children (n = 653, 427 Finns), and adults (n = 759, 538 Finns) from the United States of America and Finland. Participants from both cultures completed the Infant Behavior Questionnaire, Childhood Behavior Questionnaire and the Adult Temperament Questionnaire. Across all ages, Americans received higher ratings on temperamental fearfulness than Finnish individuals, and also demonstrated higher levels of other negative affects at several time points. During infancy and adulthood, Finns tended to score higher on positive affect and elements of temperamental effortful control. Gender differences consistent with prior studies emerged cross-culturally, and were found to be more pronounced in the US during childhood and in Finland during adulthood. © 2012 The Authors. Scandinavian Journal of Psychology © 2012 The Scandinavian Psychological Associations.

  18. Influence and adjustment goals: sources of cultural differences in ideal affect.

    PubMed

    Tsai, Jeanne L; Miao, Felicity F; Seppala, Emma; Fung, Helene H; Yeung, Dannii Y

    2007-06-01

    Previous studies have found that in American culture high-arousal positive states (HAP) such as excitement are valued more and low-arousal positive states (LAP) such as calm are valued less than they are in Chinese culture. What specific factors account for these differences? The authors predicted that when people and cultures aimed to influence others (i.e., assert personal needs and change others' behaviors to meet those needs), they would value HAP more and LAP less than when they aimed to adjust to others (i.e., suppress personal needs and change their own behaviors to meet others' needs). They test these predictions in 1 survey and 3 experimental studies. The findings suggest that within and across American and Chinese contexts, differences in ideal affect are due to specific interpersonal goals. (c) 2007 APA, all rights reserved.

  19. Assessing Parenting Behaviors in Euro-Canadian and East Asian Immigrant Mothers: Limitations to Observations of Responsiveness

    ERIC Educational Resources Information Center

    Chan, Kathy; Penner, Kailee; Mah, Janet W. T.; Johnston, Charlotte

    2010-01-01

    The use of parenting measures that are developed for use with Western families without testing their validity among families from non-Western cultural backgrounds may not be appropriate. Similar parenting behaviors may affect child outcomes in different ways across different cultures. This study examined the cross-cultural validity of an…

  20. Leaders' smiles reflect cultural differences in ideal affect.

    PubMed

    Tsai, Jeanne L; Ang, Jen Ying Zhen; Blevins, Elizabeth; Goernandt, Julia; Fung, Helene H; Jiang, Da; Elliott, Julian; Kölzer, Anna; Uchida, Yukiko; Lee, Yi-Chen; Lin, Yicheng; Zhang, Xiulan; Govindama, Yolande; Haddouk, Lise

    2016-03-01

    Cultures differ in the emotions they teach their members to value ("ideal affect"). We conducted 3 studies to examine whether leaders' smiles reflect these cultural differences in ideal affect. In Study 1, we compared the smiles of top-ranked American and Chinese government leaders, chief executive officers, and university presidents in their official photos. Consistent with findings that Americans value excitement and other high-arousal positive states more than Chinese, American top-ranked leaders (N = 98) showed more excited smiles than Chinese top-ranked leaders (N = 91) across occupations. In Study 2, we compared the smiles of winning versus losing political candidates and higher versus lower ranking chief executive officers and university presidents in the United States and Taiwan/China. American leaders (N = 223) showed more excited smiles than Taiwanese/Chinese leaders (N = 266), regardless of election outcome or ranking. In Study 3, we administered self-report measures of ideal affect in college student samples from 10 different nations (N = 1,267) and then 8 years later, coded the smiles that legislators from those nations showed in their official photos (N = 3,372). The more nations valued excitement and other high arousal positive states, the more their leaders showed excited smiles; similarly, the more nations valued calm and other low-arousal positive states, the more their leaders showed calm smiles. These results held after controlling for national differences in democratization, human development, and gross domestic product per capita. Together, these findings suggest that leaders' smiles reflect the affective states valued by their cultures. (c) 2016 APA, all rights reserved).

  1. Culture and Hallucinations: Overview and Future Directions

    PubMed Central

    Larøi, Frank; Luhrmann, Tanya Marie; Bell, Vaughan; Christian, William A.; Deshpande, Smita; Fernyhough, Charles; Jenkins, Janis; Woods, Angela

    2014-01-01

    A number of studies have explored hallucinations as complex experiences involving interactions between psychological, biological, and environmental factors and mechanisms. Nevertheless, relatively little attention has focused on the role of culture in shaping hallucinations. This article reviews the published research, drawing on the expertise of both anthropologists and psychologists. We argue that the extant body of work suggests that culture does indeed have a significant impact on the experience, understanding, and labeling of hallucinations and that there may be important theoretical and clinical consequences of that observation. We find that culture can affect what is identified as a hallucination, that there are different patterns of hallucination among the clinical and nonclinical populations, that hallucinations are often culturally meaningful, that hallucinations occur at different rates in different settings; that culture affects the meaning and characteristics of hallucinations associated with psychosis, and that the cultural variations of psychotic hallucinations may have implications for the clinical outcome of those who struggle with psychosis. We conclude that a clinician should never assume that the mere report of what seems to be a hallucination is necessarily a symptom of pathology and that the patient’s cultural background needs to be taken into account when assessing and treating hallucinations. PMID:24936082

  2. Ethnic Variation in Depressive Symptoms in a Community Sample in Hawai‘i

    PubMed Central

    Kanazawa, Asako; White, Patricia M.; Hampson, Sarah E.

    2008-01-01

    A modified CES-D was administered to a community sample of 176 European Americans (EA), 209 Native Hawaiians (NH), and 357 Japanese Americans (JA), yielding measures of depression, positive affect, depressed affect, somatic disturbance and disturbed interpersonal relations. Positive affect was lower in JA relative to EA, consistent with findings among Native Japanese, a pattern attributed to cultural variation in emotion regulation. NH reported lower positive affect than EA, accompanied by elevated negative affect and somatic disturbance, suggesting generally higher levels of depressive symptoms. The three ethnic groups varied in mental healthcare usage with differing associations between depressive symptoms and experiences of stressful life events. Taken together, these results suggest ethnic variation in depressive symptoms may arise from differing cultural beliefs. PMID:17227175

  3. Challenging the Western Approach to Cultural Comparisons: Young Pupils' Affective Structures Regarding Mathematics in Finland and Chile

    ERIC Educational Resources Information Center

    Tuohilampi, Laura; Hannula, Markku S.; Varas, Leonor; Giaconi, Valentina; Laine, Anu; Näveri, Liisa; i Nevado, Laia Saló

    2015-01-01

    Large-scale studies measure mathematics-related affect using questionnaires developed by researchers in primarily English-based countries and according to Western-based theories. Influential comparative conclusions about different cultures and countries are drawn based on such measurements. However, there are certain premises involved in these…

  4. Culturally Competent Counselors: A Self-Assessment Rubric.

    ERIC Educational Resources Information Center

    Ametrano, Irene Mass; Callaway, Yvonne L.; Stickel, Sue A.

    This paper examines graduate counseling students' affective and cognitive development related to cultural competence. Counseling Process and Cross Cultural Counseling, two required courses in a master's degree program at a regional university in the Midwest, were designed to provide a knowledge-base about cultural differences and to project how…

  5. Cultural Bridging through Shared Adventure: Cross-Cultural Perspectives on Adventure Therapy

    ERIC Educational Resources Information Center

    Norton, Christine L.; Hsieh, Chi-Mou

    2011-01-01

    This paper examines the importance of the therapeutic relationship and the need for cultural competence in adventure therapy. Cultural differences between therapist and client can sometimes result in possible misinterpretation and conflict, which can lead to problems in the therapeutic relationship and negatively affect treatment outcomes. This…

  6. Culturally Responsive Teaching Knowledge and Practices of Online Faculty

    ERIC Educational Resources Information Center

    Heitner, Keri L.; Jennings, Miranda

    2016-01-01

    Cultural differences between faculty and their students can create important challenges that affect the quality and efficacy of online teaching and learning. The objectives of this study were to: (a) create and pilot test an assessment for online faculty to measure culturally responsive teaching knowledge (CRT) and culturally responsive…

  7. Predicting attitudes toward nanotechnology: The influence of cultural and predispositional values

    NASA Astrophysics Data System (ADS)

    Shih, Tsung-Jen

    Past experience in dealing with biotechnology has suggested that public opinion plays an important role in determining the prosperity of emerging technologies. A great amount of money and energy, therefore, were invested to understand nanotechnology's impact on the society and the public, in addition to the technical advancement of the technology. However, most studies examining public opinion have focused on personal level factors and have ignored the potential influence of cultural factors. This study addresses this gap by analyzing public opinion in 21 countries, including the US and 20 European countries. Specifically, this study examines the impact of predispositional and cultural values on public support for nanotechnology, with the mediating roles of moral judgment and risk perception accounted for. This study also looks into the dynamics between cultural values and predispositional values; that is, how cultural values may moderate the effects of predispositional values in affecting attitudes toward nanotechnology. The results indicate that people rely on "information shortcuts," such as confidence and religious belief, for decision making. Individual-level factors still play an important role in shaping public attitudes even after country-level factors are controlled. Furthermore, aggregate cultural values provide people with important "mental programs" to interpret nanotechnology. They explain why people in different cultures have different moral and risk perceptions. However, most of the cultural values do not affect public support directly, suggesting that public support is contingent greatly on the core characteristics of nanotechnology, such as its usefulness, risk, and moral acceptability, which, in turn, is influenced by personal beliefs and cultural givens. The results also suggest that people in different cultures respond to survey questions in different manners. People living in cultures emphasizing uncertainty avoidance and individualism are more likely to provide substantive answers. Conversely, those living in cultures emphasizing masculinity have a higher chance of answering "don't know." In addition to these cultural factors, the lack of knowledge, ambivalence, and attitudes toward other controversial technologies at the personal level are also found to affect people's ability to provide substantive answers. These findings have significant implications for cross-cultural communication and risk communication with respect to emerging technologies.

  8. Leaders’ Smiles Reflect Cultural Differences in Ideal Affect

    PubMed Central

    Tsai, Jeanne L.; Ang, Jen Ying Zhen; Blevins, Elizabeth; Goernandt, Julia; Fung, Helene H.; Jiang, Da; Elliott, Julian; Kölzer, Anna; Uchida, Yukiko; Lee, Yi-Chen; Lin, Yicheng; Zhang, Xiulan; Govindama, Yolande; Haddouk, Lise

    2015-01-01

    Cultures differ in the emotions they teach their members to value (“ideal affect”). We conducted three studies to examine whether leaders’ smiles reflect these cultural differences in ideal affect. In Study 1, we compared the smiles of top ranked American and Chinese government leaders, chief-executive-officers (CEOs), and university presidents in their official photos. Consistent with findings that Americans value excitement and other high arousal positive states more than Chinese, American top ranked leaders (N = 98) showed more excited smiles than Chinese top ranked leaders (N = 91) across occupations. In Study 2, we compared the smiles of winning vs. losing political candidates and higher vs. lower ranking CEOs and university presidents in the US and Taiwan/China. American leaders (N = 223) showed more excited smiles than Taiwanese/Chinese leaders (N =266), regardless of election outcome or ranking. In Study 3, we administered self-report measures of ideal affect in college student samples from 10 different nations (N = 1,267) and then eight years later, coded the smiles that legislators from those nations showed in their official photos (N = 3,372). The more nations valued excitement and other high arousal positive states, the more their leaders showed excited smiles; similarly, the more nations valued calm and other low arousal positive states, the more their leaders showed calm smiles. These results held after controlling for national differences in GDP per capita, democratization, and human development. Together, these findings suggest that leaders’ smiles reflect the affective states valued by their cultures. PMID:26751631

  9. Affective, Normative, and Continuance Commitment Levels across Cultures: A Meta-Analysis

    ERIC Educational Resources Information Center

    Meyer, John P.; Stanley, David J.; Jackson, Timothy A.; McInnis, Kate J.; Maltin, Elyse R.; Sheppard, Leah

    2012-01-01

    With increasing globalization of business and diversity within the workplace, there has been growing interest in cultural differences in employee commitment. We used meta-analysis to compute mean levels of affective (AC; K=966, N=433,129), continuance (CC; K=428, N=199,831), and normative (NC; K=336, N=133,277) organizational commitment for as…

  10. Cultural Sensitivity Levels of University Students Receiving Education in Health Disciplines

    ERIC Educational Resources Information Center

    Meydanlioglu, Ayse; Arikan, Fatma; Gozum, Sebahat

    2015-01-01

    Medical and nursing students are expected to be more competent in terms of being acquainted with different cultures and approaching culturally, compared to many other disciplines. This descriptive study was designed to evaluate the cultural sensitivity levels of nursing and medical students and the affecting factors. One hundred and eleven nursing…

  11. Addressing Cross-Cultural Teamwork Barriers: Implications for Industry Practice and Higher Education Curricula

    ERIC Educational Resources Information Center

    Levitt, Steven R.

    2016-01-01

    This study explores cultural factors affecting international team dynamics and the implications for industry practice and higher education. Despite decades of studying and experience with cultural diversity, international work groups continue to be challenged by ethnocentrism and prejudices. Central to the context is that cultural differences in…

  12. Focusing on the Negative: Cultural Differences in Expressions of Sympathy

    PubMed Central

    Koopmann-Holm, Birgit; Tsai, Jeanne L.

    2014-01-01

    Feeling concern about the suffering of others is considered a basic human response, and yet, we know surprisingly little about the cultural factors that shape how people respond to the suffering of another person. To this end, we conducted four studies that tested the hypothesis that American expressions of sympathy focus on the negative less and positive more than German expressions of sympathy, in part because Americans want to avoid negative states more than Germans do. In Study 1, we demonstrate that American sympathy cards contained less negative and more positive content than German sympathy cards. In Study 2, we show that European Americans want to avoid negative states more than Germans do. In Study 3, we demonstrate that these cultural differences in “avoided negative affect” mediate cultural differences in how comfortable Americans and Germans felt focusing on the negative (vs. positive) when expressing sympathy for the hypothetical death of an acquaintance's father. To examine whether greater avoided negative affect results in lesser focus on the negative and greater focus on the positive when responding to another person's suffering, in Study 4, American and German participants were randomly assigned to one of two conditions: (1) to “push negative images away” (i.e., increasing desire to avoid negative affect), or (2) to “pull negative images closer” to themselves (i.e., decreasing desire to avoid negative affect). Participants were then asked to pick a card to send to an acquaintance whose father had hypothetically just died. Across cultures, participants in the “push negative away” condition were less likely to choose sympathy cards with negative (vs. positive) content than were those in the “pull negative closer” condition. Together, these studies suggest that cultures differ in their desire to avoid negative affect, and that these differences influence the degree to which expressions of sympathy focus on the negative (vs. positive). We discuss the implications of these findings for current models of sympathy, compassion, and helping. PMID:25243416

  13. Effects of bonding with parents and home culture on intercultural adaptations and the moderating role of genes.

    PubMed

    Phua, Desiree Y; Meaney, Michael J; Khor, Chiea Chuen; Lau, Ivy Y M; Hong, Ying-Yi

    2017-05-15

    In the current age of globalization, living abroad is becoming an increasingly common and highly sought after experience. Sojourners' ability to adjust to a new culture can be affected by their existing attachments, internalized as intrapsychic environment, as well as their biological sensitivity to environment. This sensitivity can be partly attributed to one's genomic endowments. As such, this prospective study sought to examine the differential effects of early experiences with parents and affection for home culture on young adults' ability to adapt to a foreign culture (n=305, students who studied overseas for a semester) - specifically, the difficulties they experience - moderated by genetic susceptibility. An additional 258 students who did not travel overseas were included as a comparison group to demonstrate the uniqueness of intercultural adaptation. Current findings suggest that the maternal, paternal and cultural bondings or affections affect different aspects of intercultural adjustment. Maternal bonding affected sojourners' relationships with host nationals, while paternal bonding affected sojourners' adjustment to a new physical environment. Moreover, individuals' genetic predispositions significantly moderate these main effects regarding how much difficulty the sojourners experienced overseas. Copyright © 2017 Elsevier B.V. All rights reserved.

  14. Attention to eye contact in the West and East: autonomic responses and evaluative ratings.

    PubMed

    Akechi, Hironori; Senju, Atsushi; Uibo, Helen; Kikuchi, Yukiko; Hasegawa, Toshikazu; Hietanen, Jari K

    2013-01-01

    Eye contact has a fundamental role in human social interaction. The special appearance of the human eye (i.e., white sclera contrasted with a coloured iris) implies the importance of detecting another person's face through eye contact. Empirical studies have demonstrated that faces making eye contact are detected quickly and processed preferentially (i.e., the eye contact effect). Such sensitivity to eye contact seems to be innate and universal among humans; however, several studies suggest that cultural norms affect eye contact behaviours. For example, Japanese individuals exhibit less eye contact than do individuals from Western European or North American cultures. However, how culture modulates eye contact behaviour is unclear. The present study investigated cultural differences in autonomic correlates of attentional orienting (i.e., heart rate) and looking time. Additionally, we examined evaluative ratings of eye contact with another real person, displaying an emotionally neutral expression, between participants from Western European (Finnish) and East Asian (Japanese) cultures. Our results showed that eye contact elicited stronger heart rate deceleration responses (i.e., attentional orienting), shorter looking times, and higher ratings of subjective feelings of arousal as compared to averted gaze in both cultures. Instead, cultural differences in the eye contact effect were observed in various evaluative responses regarding the stimulus faces (e.g., facial emotion, approachability etc.). The rating results suggest that individuals from an East Asian culture perceive another's face as being angrier, unapproachable, and unpleasant when making eye contact as compared to individuals from a Western European culture. The rating results also revealed that gaze direction (direct vs. averted) could influence perceptions about another person's facial affect and disposition. These results suggest that cultural differences in eye contact behaviour emerge from differential display rules and cultural norms, as opposed to culture affecting eye contact behaviour directly at the physiological level.

  15. Attention to Eye Contact in the West and East: Autonomic Responses and Evaluative Ratings

    PubMed Central

    Akechi, Hironori; Senju, Atsushi; Uibo, Helen; Kikuchi, Yukiko; Hasegawa, Toshikazu; Hietanen, Jari K.

    2013-01-01

    Eye contact has a fundamental role in human social interaction. The special appearance of the human eye (i.e., white sclera contrasted with a coloured iris) implies the importance of detecting another person's face through eye contact. Empirical studies have demonstrated that faces making eye contact are detected quickly and processed preferentially (i.e., the eye contact effect). Such sensitivity to eye contact seems to be innate and universal among humans; however, several studies suggest that cultural norms affect eye contact behaviours. For example, Japanese individuals exhibit less eye contact than do individuals from Western European or North American cultures. However, how culture modulates eye contact behaviour is unclear. The present study investigated cultural differences in autonomic correlates of attentional orienting (i.e., heart rate) and looking time. Additionally, we examined evaluative ratings of eye contact with another real person, displaying an emotionally neutral expression, between participants from Western European (Finnish) and East Asian (Japanese) cultures. Our results showed that eye contact elicited stronger heart rate deceleration responses (i.e., attentional orienting), shorter looking times, and higher ratings of subjective feelings of arousal as compared to averted gaze in both cultures. Instead, cultural differences in the eye contact effect were observed in various evaluative responses regarding the stimulus faces (e.g., facial emotion, approachability etc.). The rating results suggest that individuals from an East Asian culture perceive another's face as being angrier, unapproachable, and unpleasant when making eye contact as compared to individuals from a Western European culture. The rating results also revealed that gaze direction (direct vs. averted) could influence perceptions about another person's facial affect and disposition. These results suggest that cultural differences in eye contact behaviour emerge from differential display rules and cultural norms, as opposed to culture affecting eye contact behaviour directly at the physiological level. PMID:23516627

  16. Cultural Adaptation of a Neurobiologically Informed Intervention in Local and International Contexts.

    PubMed

    Pakulak, Eric; Hampton Wray, Amanda; Longoria, Zayra; Garcia Isaza, Alejandra; Stevens, Courtney; Bell, Theodore; Burlingame, Sarah; Klein, Scott; Berlinski, Samuel; Attanasio, Orazio; Neville, Helen

    2017-12-01

    The relationship between early adversity and numerous negative outcomes across the lifespan is evident in a wide range of societies and cultures (e.g., Pakulak, Stevens, & Neville, 2018). Among the most affected neural systems are those supporting attention, self-regulation, and stress regulation. As such, these systems represent targets for neurobiologically informed interventions addressing early adversity. In prior work with monolingual native English-speaking families, we showed that a two-generation intervention targeting these systems in families improves outcomes across multiple domains including child brain function for selective attention (for detail, see Neville et al., 2013). Here, we discuss the translation and cultural adaptation (CA) of this intervention in local and international contexts, which required systematic consideration of cultural differences that could affect program acceptability. First, we conducted a translation and CA of our program to serve Latino families in the United States using the Cultural Adaptation Process (CAP), a model that works closely with stakeholders in a systematic, iterative process. Second, to implement the adapted program in Medellín, Colombia, we conducted a subsequent adaptation for Colombian culture using the same CAP. Our experience underscores the importance of consideration of cultural differences and a systematic approach to adaptation before assessing the efficacy of neurobiologically informed interventions in different cultural contexts. © 2017 Wiley Periodicals, Inc.

  17. Culture and hallucinations: overview and future directions.

    PubMed

    Larøi, Frank; Luhrmann, Tanya Marie; Bell, Vaughan; Christian, William A; Deshpande, Smita; Fernyhough, Charles; Jenkins, Janis; Woods, Angela

    2014-07-01

    A number of studies have explored hallucinations as complex experiences involving interactions between psychological, biological, and environmental factors and mechanisms. Nevertheless, relatively little attention has focused on the role of culture in shaping hallucinations. This article reviews the published research, drawing on the expertise of both anthropologists and psychologists. We argue that the extant body of work suggests that culture does indeed have a significant impact on the experience, understanding, and labeling of hallucinations and that there may be important theoretical and clinical consequences of that observation. We find that culture can affect what is identified as a hallucination, that there are different patterns of hallucination among the clinical and nonclinical populations, that hallucinations are often culturally meaningful, that hallucinations occur at different rates in different settings; that culture affects the meaning and characteristics of hallucinations associated with psychosis, and that the cultural variations of psychotic hallucinations may have implications for the clinical outcome of those who struggle with psychosis. We conclude that a clinician should never assume that the mere report of what seems to be a hallucination is necessarily a symptom of pathology and that the patient's cultural background needs to be taken into account when assessing and treating hallucinations. © The Author 2014. Published by Oxford University Press on behalf of the Maryland Psychiatric Research Center.

  18. The cytoskeleton of Drosophila-derived Schneider line-1 and Kc23 cells undergoes significant changes during long-term culture

    NASA Technical Reports Server (NTRS)

    Schatten, H.; Hedrick, J.; Chakrabarti, A.

    1998-01-01

    Insect cell cultures derived from Drosophila melanogaster are increasingly being used as an alternative system to mammalian cell cultures, as they are amenable to genetic manipulation. Although Drosophila cells are an excellent tool for the study of genes and expression of proteins, culture conditions have to be considered in the interpretation of biochemical results. Our studies indicate that significant differences occur in cytoskeletal structure during the long-term culture of the Drosophila-derived cell lines Schneider Line-1 (S1) and Kc23. Scanning, transmission-electron, and immunofluorescence microscopy studies reveal that microfilaments, microtubules, and centrosomes become increasingly different during the culture of these cells from 24 h to 7-14 days. Significant cytoskeletal changes are observed at the cell surface where actin polymerizes into microfilaments, during the elongation of long microvilli. Additionally, long protrusions develop from the cell surface; these protrusions are microtubule-based and establish contact with neighboring cells. In contrast, the microtubule network in the interior of the cells becomes disrupted after four days of culture, resulting in altered transport of mitochondria. Microtubules and centrosomes are also affected in a small percent of cells during cell division, indicating an instability of centrosomes. Thus, the cytoskeletal network of microfilaments, microtubules, and centrosomes is affected in Drosophila cells during long-term culture. This implies that gene regulation and post-translational modifications are probably different under different culture conditions.

  19. Dietary potassium diformate did not affect growth and survival but did reduce nutrient digestibility of Pacific white shrimp cultured under clean water conditions

    USDA-ARS?s Scientific Manuscript database

    This study investigated the effect of a dietary supplement potassium diformate (PDF) on growth performance, survival and nutrient digestibility of Pacific white shrimp cultured under clean water conditions. We found that weight gain was not significantly (P>0.05) affected by the different levels of ...

  20. Bridging a Cultural Divide

    ERIC Educational Resources Information Center

    Previdi, Patricia; Belfrage, Mary; Hu, Florence

    2005-01-01

    Playing an active role in a child's education can be especially difficult for parents of English Language Learners (ELL) and Limited English Proficiency (LEP) students. Differences in cultural beliefs and language are often barriers to effective parent-school interaction. Such cultural discontinuities between home and school can affect a child's…

  1. The effects of corporate social responsibility on employees' affective commitment: a cross-cultural investigation.

    PubMed

    Mueller, Karsten; Hattrup, Kate; Spiess, Sven-Oliver; Lin-Hi, Nick

    2012-11-01

    This study investigated the moderating effects of several Global Leadership and Organizational Behavior Effectiveness (GLOBE) cultural value dimensions on the relationship between employees' perceptions of their organization's social responsibility and their affective organizational commitment. Based on data from a sample of 1,084 employees from 17 countries, results showed that perceived corporate social responsibility (CSR) was positively related to employees' affective commitment (AC), after controlling for individual job satisfaction and gender as well as for nation-level differences in unemployment rates. In addition, several GLOBE value dimensions moderated the effects of CSR on AC. In particular, perceptions of CSR were more positively related to AC in cultures higher in humane orientation, institutional collectivism, ingroup collectivism, and future orientation and in cultures lower in power distance. Implications for future CSR research and cross-cultural human resources management are discussed. (c) 2012 APA, all rights reserved.

  2. Cultural relativity in perceiving emotion from vocalizations

    PubMed Central

    Gendron, Maria; Roberson, Debi; van der Vyver, Jacoba Marietta; Barrett, Lisa Feldman

    2014-01-01

    A central question in the study of human behavior is whether or not certain emotions, such as anger, fear and sadness, are recognized across cultures in non-verbal cues. We predicted and found that in a concept-free experimental task, participants from an isolated cultural context (the Himba ethnic group from Northwest Namibia) do not freely label Western vocalizations with expected emotion terms. Responses indicated Himba participants perceived more basic affective properties of valence (positivity or negativity) and to some extent arousal (high or low activation). In a second concept-embedded task, we manipulated whether a given trial could be solved using only affective content or discrete emotion content based on the foil choice. Above chance accuracy in Himba participants occurred only when foils differed from targets in valence, indicating that the voice can reliably convey affective meaning across cultures, but that perceptions of emotion from the voice are culturally variable. PMID:24501109

  3. Influence of Culture in Obsessive-compulsive Disorder and Its Treatment

    PubMed Central

    Nicolini, Humberto; Salin-Pascual, Rafael; Cabrera, Brenda; Lanzagorta, Nuria

    2017-01-01

    Background: The aspects of cultural identity and its impact on obsessive-compulsive disorder (OCD) have been un-derstudied. There are different opinions, ranging from the idea that culture does not affect the symptoms of this condition to the idea that cultures with high religiosity may have more severity of OCD. Also, the concept of OCD has considerably var-ied across history and cultures, from being considered an issue related to lack of control of blasphemous ideas, and a part of anxious issues, to the description of complex neurobiological systems in its causation. Objective: The aim of this review was to address OCD as a well-characterized disorder with a proposed neurobiological ba-sis which may or may not have variations depending on cultural diversity. The question that was asked in this review is whether or not there are cultural differences in the manifestations of the OCD symptomatology and which factors of cultural diversity have a major influence on such manifestations along with the differences among some cultures regarding OCD is-sues, where the difference among countries has also been highlighted. Methods: A review of the literature was conducted that includes the following words: obsessive-compulsive disorder, cul-ture, cultural identity and religion in a period of 10 years. Conclusion: Cultural variations do not seem to differ from symptomatic clusters of OCD, which may be indicating that a se-ries of adaptive behaviors is evolutionarily evolving to be constantly altered, perhaps by well-determined pathophysiological mechanisms. Some aspects that have been related to some dimensions of OCD symptomatology are religion and religiosity, affecting the content of obsessions and the severity of manifestations. Properly evaluating the education background, access to health services, food, and the genetic structure of populations, using investigational instruments sensitive to these cultural elements, will increase our understanding of the importance of culture on OCD and its treatment. PMID:29657563

  4. Influence of Culture in Obsessive-compulsive Disorder and Its Treatment.

    PubMed

    Nicolini, Humberto; Salin-Pascual, Rafael; Cabrera, Brenda; Lanzagorta, Nuria

    2017-12-01

    The aspects of cultural identity and its impact on obsessive-compulsive disorder (OCD) have been un-derstudied. There are different opinions, ranging from the idea that culture does not affect the symptoms of this condition to the idea that cultures with high religiosity may have more severity of OCD. Also, the concept of OCD has considerably var-ied across history and cultures, from being considered an issue related to lack of control of blasphemous ideas, and a part of anxious issues, to the description of complex neurobiological systems in its causation. The aim of this review was to address OCD as a well-characterized disorder with a proposed neurobiological ba-sis which may or may not have variations depending on cultural diversity. The question that was asked in this review is whether or not there are cultural differences in the manifestations of the OCD symptomatology and which factors of cultural diversity have a major influence on such manifestations along with the differences among some cultures regarding OCD is-sues, where the difference among countries has also been highlighted. A review of the literature was conducted that includes the following words: obsessive-compulsive disorder, cul-ture, cultural identity and religion in a period of 10 years. Cultural variations do not seem to differ from symptomatic clusters of OCD, which may be indicating that a se-ries of adaptive behaviors is evolutionarily evolving to be constantly altered, perhaps by well-determined pathophysiological mechanisms. Some aspects that have been related to some dimensions of OCD symptomatology are religion and religiosity, affecting the content of obsessions and the severity of manifestations. Properly evaluating the education background, access to health services, food, and the genetic structure of populations, using investigational instruments sensitive to these cultural elements, will increase our understanding of the importance of culture on OCD and its treatment.

  5. The impact of cultural differences in self-representation on the neural substrates of posttraumatic stress disorder

    PubMed Central

    Liddell, Belinda J.; Jobson, Laura

    2016-01-01

    A significant body of literature documents the neural mechanisms involved in the development and maintenance of posttraumatic stress disorder (PTSD). However, there is very little empirical work considering the influence of culture on these underlying mechanisms. Accumulating cultural neuroscience research clearly indicates that cultural differences in self-representation modulate many of the same neural processes proposed to be aberrant in PTSD. The objective of this review paper is to consider how culture may impact on the neural mechanisms underlying PTSD. We first outline five key affective and cognitive functions and their underlying neural correlates that have been identified as being disrupted in PTSD: (1) fear dysregulation; (2) attentional biases to threat; (3) emotion and autobiographical memory; (4) self-referential processing; and (5) attachment and interpersonal processing. Second, we consider prominent cultural theories and review the empirical research that has demonstrated the influence of cultural variations in self-representation on the neural substrates of these same five affective and cognitive functions. Finally, we propose a conceptual model that suggests that these five processes have major relevance to considering how culture may influence the neural processes underpinning PTSD. Highlights of the article Cultural variations in individualistic-collectivistic self-representation modulate many of the same neural and psychological processes disrupted in PTSD. These commonly affected processes include fear perception and regulation mechanisms, attentional biases (to threat), emotional and autobiographical memory systems, self-referential processing and attachment systems. A conceptual model is proposed whereby culture is considered integral to the development and maintenance of PTSD and its neural substrates. PMID:27302635

  6. Good feelings in christianity and buddhism: religious differences in ideal affect.

    PubMed

    Tsai, Jeanne L; Miao, Felicity F; Seppala, Emma

    2007-03-01

    Affect valuation theory (AVT) predicts cultural variation in the affective states that people ideally want to feel (i.e., "ideal affect"). National and ethnic comparisons support this prediction: For instance, European Americans (EA) value high arousal positive (HAP) states (e.g., excitement) more and low arousal positive (LAP) states (e.g., calm) less than Hong Kong Chinese. In this article, the authors examine whether religions differ in the ideal affective states they endorse. The authors predicted that Christianity values HAP more and LAP less than Buddhism. In Study 1, they compared Christian and Buddhist practitioners' ideal affect. In Studies 2 and 3, they compared the endorsement of HAP and LAP in Christian and Buddhist classical texts (e.g., Gospels, Lotus Sutra) and contemporary self-help books (e.g., Your Best Life Now, Art of Happiness). Findings supported predictions, suggesting that AVT applies to religious and to national and ethnic cultures.

  7. Cross-Cultural Differences in Childrearing Goals.

    ERIC Educational Resources Information Center

    Hoffman, Lois Wladis

    1988-01-01

    Data from eight countries were analyzed to explore hypotheses about cross-cultural differences in childrearing patterns. Particular attention is given to LeVine's and Kohn's theories, and Hoffman and Hoffman's new theory that contends that children satisfy certain parental needs and that the satisfaction of specific needs affects parents'…

  8. Cross Cultural Differences in Online Learning Motivation

    ERIC Educational Resources Information Center

    Lim, Doo H.

    2004-01-01

    Globalization and technology are two of the many drivers that impact today's education, locally and internationally. The purpose of the research study was to identify how online learners in Korea and the US perceived online learning motivation differently and what learner characteristics and cultural orientation affected the online learners'…

  9. Cross Cultural Differences in Online Learning Motivation

    ERIC Educational Resources Information Center

    Lim, Doo H.

    2004-01-01

    Globalization and technology are two of the many drivers that impact today's education locally and internationally. The purpose of the research study was to identify how online learners in Korea and the U.S. perceived online learning motivation differently and what learner characteristics and cultural orientation affected the online learners'…

  10. Culture as common sense: perceived consensus versus personal beliefs as mechanisms of cultural influence.

    PubMed

    Zou, Xi; Tam, Kim-Pong; Morris, Michael W; Lee, Sau-Lai; Lau, Ivy Yee-Man; Chiu, Chi-Yue

    2009-10-01

    The authors propose that culture affects people through their perceptions of what is consensually believed. Whereas past research has examined whether cultural differences in social judgment are mediated by differences in individuals' personal values and beliefs, this article investigates whether they are mediated by differences in individuals' perceptions of the views of people around them. The authors propose that individuals who perceive that traditional views are culturally consensual (e.g., Chinese participants who believe that most of their fellows hold collectivistic values) will themselves behave and think in culturally typical ways. Four studies of previously well-established cultural differences found that cultural differences were mediated by participants' perceived consensus as much as by participants' personal views. This held true for cultural differences in the bases of compliance (Study 1), attributional foci (Study 2), and counterfactual thinking styles (Study 3). To tease apart the effect of consensus perception from other possibly associated individual differences, in Study 4, the authors experimentally manipulated which of 2 cultures was salient to bicultural participants and found that judgments were guided by participants' perception of the consensual view of the salient culture. 2009 APA, all rights reserved.

  11. Production of novel types of antibacterial liamocins by diverse strains of Aureobasidium pullulans grown on different culture media.

    PubMed

    Leathers, Timothy D; Price, Neil P J; Bischoff, Kenneth M; Manitchotpisit, Pennapa; Skory, Christopher D

    2015-10-01

    To compare production of antibacterial liamocins (polyol lipids) by diverse strains of Aureobasidium pullulans grown on different culture media. Liamocins produced by strains of A. pullulans have potential agricultural and pharmaceutical applications as antibacterials with specificity against Streptococcus spp. Six strains of A. pullulans were characterized for liamocin production on four different culture media. The choice of strain and culture medium affected growth, liamocin yields, and production of contaminating pigments. Best growth and highest liamocin yields were obtained using A. pullulans strain NRRL 50384 grown on a sucrose basal medium. Unexpectedly, the choice of strain and culture medium also affected the structure of liamocins produced, providing novel types of liamocins. Liamocins varied not only in the ratios of trimer and tetramer polyester tail groups, but also in the nature of the polyol headgroup, which could include mannitol, arabitol, or glycerol. The ability to conveniently produce novel types of liamocins in good yields will provide novel antibacterials for applied uses, and facilitate structure-function studies on the mechanism of antibacterial activity.

  12. Effect of culture medium on toxic effect of ZnO nanoparticles to freshwater microalgae

    NASA Astrophysics Data System (ADS)

    Aravantinou, Andriana F.; Tsarpali, Vasiliki; Dailianis, Stefanos; Manariotis, Ioannis D.

    2014-05-01

    The widely use of nanoparticles (NPs) in many products, is increasing over time. The release of NPs into the environment may affect ecosystems, and therefore it is essential to study their impact on aquatic organisms. The aim of this work was to investigate the effect of zinc oxide (ZnO) NPs on microalgae, cultured in different mediums. Chlorococcum sp. and Scenedesmus rubescens were used as freshwater microalgae model species in order to investigate the toxic effects of ZnO NPs. Microalgae species exposed to ZnO NPs concentrations varying from 0.081 to 810 mg/L for different periods of time (24 to 96 h) and two different culture mediums. The aggregation level and particle size distribution of NPs were also determined during the experiments. The experimental results revealed significant differences on algae growth rates depending on the selected culture medium. Specifically, the toxic effect of ZnO NPs in Chlorococcum sp. was higher in cultures with 1/3N BG-11 medium than in BBM medium, despite the fact that the dissolved zinc concentration was higher in BBM medium. On the other hand, Scenedesmus rubescens exhibited the exact opposite behavior, with the highest toxic effect in cultures with BBM medium. Both species growth was significantly affected by the exposure time, the NPs concentrations, and mainly the culture medium.

  13. Cultural and Social Factors Affecting Women's Physical Activity Participation in Taiwan

    ERIC Educational Resources Information Center

    Yu, Chia-Chen; Liaw, Yin-Hua; Barnd, Susan

    2004-01-01

    The world is becoming a global village and within many professions, including physical education and sport, individuals have multiple opportunities to work and socialize with people from different ethnic backgrounds, cultures, lifestyles, religions, etc. Due to the increasing cultural diversity, it is important for physical education and sport…

  14. Cultural Factors in Learning and Education.

    ERIC Educational Resources Information Center

    Aboud, Frances E., Ed.; Meade, Robert D., Ed.

    The Fifth Western Symposium on Learning, held November 15 and 16, 1973, considered the question of how cultural factors affect learning that produces important differences in personality. The following papers, with commentaries, are collected in this volume: (1) "Cultural Factors in Learning and Education: Opening Remarks," by Paul Woodring; (2)…

  15. Factors that Affect the Decision of Refugee and Immigrant Students to Pursue Higher Education in Tennessee: The Case of Egyptian, Somali, Kurdish and Mexican Students

    ERIC Educational Resources Information Center

    Weldegebriel, Mengistu H.

    2011-01-01

    Refugee and immigrant students face challenges in pursuing higher education. This study examined factors that affect the decision of refugees and immigrant students to pursue higher education in Tennessee. The factors included cultural capital these students acquire from their parents and families; cultural difference they have with the mainstream…

  16. Cultural aspects of communication in cancer care.

    PubMed

    Surbone, A

    2006-01-01

    Cancer is increasing in incidence and prevalence worldwide, and the WHO has recently included cancer and its treatments as a health priority in developed and developing countries. The cultural diversity of oncology patients is bound to increase, and cultural sensitivity and competence are now required of all oncology professionals. A culturally competent cancer care leads to improved therapeutic outcome and it may decrease disparities in medical care. Cultural competence in medicine is a complex multilayered accomplishment, requiring knowledge, skills and attitudes whose acquisition is needed for effective cross-cultural negotiation in the clinical setting. Effective cultural competence is based on knowledge of the notion of culture; on awareness of possible biases and prejudices related to stereotyping, racism, classism, sexism; on nurturing appreciation for differences in health care values; and on fostering the attitudes of humility, empathy, curiosity, respect, sensitivity and awareness. Cultural competence in healthcare relates to individual professionals, but also to organizations and systems. A culturally competent healthcare system must consider in their separateness and yet in there reciprocal influences social, racial and cultural factors. By providing a framework of reference to interpret the external world and relate to it, culture affects patients' perceptions of disease, disability and suffering; degrees and expressions of concern about them; their responses to treatments and their relationship to individual physicians and to the healthcare system. Culture also influences the interpretation of ethical norms and principles, and especially of individual autonomy, which can be perceived either as synonymous with freedom or with isolation depending on the cultural context. This, in turn, determines the variability of truth-telling attitudes and practices worldwide as well as the different roles of family in the information and decision-making process of the cancer patient. Finally, culture affects individual views of the patient-doctor relationship in different contexts.

  17. Lay theory of race affects and moderates Asian Americans' responses toward American culture.

    PubMed

    No, Sun; Hong, Ying-yi; Liao, Hsin-Ya; Lee, Kyoungmi; Wood, Dustin; Chao, Melody Manchi

    2008-10-01

    People may hold different understandings of race that might affect how they respond to the culture of groups deemed to be racially distinct. The present research tests how this process is moderated by the minority individual's lay theory of race. An essentialist lay theory of race (i.e., that race reflects deep-seated, inalterable essence and is indicative of traits and ability) would orient racial minorities to rigidly adhere to their ethnic culture, whereas a social constructionist lay theory of race (i.e., that race is socially constructed, malleable, and arbitrary) would orient racial minorities to identify and cognitively assimilate toward the majority culture. To test these predictions, the authors conducted 4 studies with Asian American participants. The first 2 studies examine the effect of one's lay theory of race on perceived racial differences and identification with American culture. The last 2 studies tested the moderating effect of lay theory of race on identification and assimilation toward the majority American culture after this culture had been primed. The results generally supported the prediction that the social constructionist theory was associated with more perceived similarity between Asians and Americans and more consistent identification and assimilation toward American culture, compared with the essentialist theory.

  18. The impact of cultural differences in self-representation on the neural substrates of posttraumatic stress disorder.

    PubMed

    Liddell, Belinda J; Jobson, Laura

    2016-01-01

    A significant body of literature documents the neural mechanisms involved in the development and maintenance of posttraumatic stress disorder (PTSD). However, there is very little empirical work considering the influence of culture on these underlying mechanisms. Accumulating cultural neuroscience research clearly indicates that cultural differences in self-representation modulate many of the same neural processes proposed to be aberrant in PTSD. The objective of this review paper is to consider how culture may impact on the neural mechanisms underlying PTSD. We first outline five key affective and cognitive functions and their underlying neural correlates that have been identified as being disrupted in PTSD: (1) fear dysregulation; (2) attentional biases to threat; (3) emotion and autobiographical memory; (4) self-referential processing; and (5) attachment and interpersonal processing. Second, we consider prominent cultural theories and review the empirical research that has demonstrated the influence of cultural variations in self-representation on the neural substrates of these same five affective and cognitive functions. Finally, we propose a conceptual model that suggests that these five processes have major relevance to considering how culture may influence the neural processes underpinning PTSD.

  19. Workshop V: Cultural Perception and Bias/Science Practice and Ethics

    NASA Astrophysics Data System (ADS)

    Chuan, Kwek Leong; Lin, Jauyn Grace; Pierron-Bohnes, Veronique; Dawson, Silvina Ponce

    2015-12-01

    Despite the objectivity of science, the local work environment affects the daily activities of scientists. Differences in cultural perception can affect female scientists in the workplace directly. The pressure currently exerted on researchers, on the other hand, is altering how science is practiced and seems to affect women and men differently. In this paper we summarize the discussions that took place on this topic in Workshop V of the 5th IUPAP International Conference on Women in Physics. We present some of the results of the 2010 Global Survey of Physicists analyzed by region and data from France and Taiwan. We also include the recommendations that were formulated at the end of the workshop.

  20. Television Images: Exploring How They Affect People's View of Self and Others

    ERIC Educational Resources Information Center

    Alexandrin, Julie R.

    2009-01-01

    Through television, many different images of ethnic, cultural, and ability groups are presented. Different people perceive these images in different ways. These perceptions affect how people value themselves and judge and interact with others. This article first summaries research on TV images and people's meaning and reaction to them. Second, it…

  1. Cultural differences in human brain activity: a quantitative meta-analysis.

    PubMed

    Han, Shihui; Ma, Yina

    2014-10-01

    Psychologists have been trying to understand differences in cognition and behavior between East Asian and Western cultures within a single cognitive framework such as holistic versus analytic or interdependent versus independent processes. However, it remains unclear whether cultural differences in multiple psychological processes correspond to the same or different neural networks. We conducted a quantitative meta-analysis of 35 functional MRI studies to examine cultural differences in brain activity engaged in social and non-social processes. We showed that social cognitive processes are characterized by stronger activity in the dorsal medial prefrontal cortex, lateral frontal cortex and temporoparietal junction in East Asians but stronger activity in the anterior cingulate, ventral medial prefrontal cortex and bilateral insula in Westerners. Social affective processes are associated with stronger activity in the right dorsal lateral frontal cortex in East Asians but greater activity in the left insula and right temporal pole in Westerners. Non-social processes induce stronger activity in the left inferior parietal cortex, left middle occipital and left superior parietal cortex in East Asians but greater activations in the right lingual gyrus, right inferior parietal cortex and precuneus in Westerners. The results suggest that cultural differences in social and non-social processes are mediated by distinct neural networks. Moreover, East Asian cultures are associated with increased neural activity in the brain regions related to inference of others' mind and emotion regulation whereas Western cultures are associated with enhanced neural activity in the brain areas related to self-relevance encoding and emotional responses during social cognitive/affective processes. Copyright © 2014 Elsevier Inc. All rights reserved.

  2. Cultural invariance of goal orientation and self-efficacy in New Zealand: Relations with achievement.

    PubMed

    Meissel, Kane; Rubie-Davies, Christine M

    2016-03-01

    There is substantial evidence indicating that various psychological processes are affected by cultural context, but such research is comparatively nascent within New Zealand. As there are four large cultural groups in New Zealand, representing an intersection of individualist, collectivist, indigenous, colonial, and immigrant cultures, New Zealand is an important context in which to investigate the role of culture in such processes. This study investigated goal orientation and self-efficacy beliefs among students of different cultural backgrounds in New Zealand, associations between motivational beliefs and achievement, and whether any relations differed by cultural background. Participants were 2,210 students attending three intermediate schools. Participants responded to a questionnaire at the beginning of the school year to evaluate self-efficacy for mathematics and mastery and performance goal orientation. Participants also completed a standardized mathematics achievement test at the beginning and end of the year. The factor structure was sufficiently invariant by cultural group, but with statistically significant differences in average level of endorsement. Self-efficacy for mathematics predicted marginally higher end-of-year achievement after controlling for beginning-of-year achievement, with a stronger relationship for Māori and Pasifika, but no statistically significant relationship with achievement among Asian students. The questionnaire used was a valid instrument for the four main cultural groups in New Zealand. Differences were found in motivation levels, and Māori and Pasifika were more affected by their self-reported self-efficacy. Teachers may be able to raise students' self-beliefs by conveying high expectations for these students, potentially supporting higher academic outcomes. © 2015 The British Psychological Society.

  3. Comparative analysis of metagenomes from three methanogenic hydrocarbon-degrading enrichment cultures with 41 environmental samples.

    PubMed

    Tan, Boonfei; Fowler, S Jane; Abu Laban, Nidal; Dong, Xiaoli; Sensen, Christoph W; Foght, Julia; Gieg, Lisa M

    2015-09-01

    Methanogenic hydrocarbon metabolism is a key process in subsurface oil reservoirs and hydrocarbon-contaminated environments and thus warrants greater understanding to improve current technologies for fossil fuel extraction and bioremediation. In this study, three hydrocarbon-degrading methanogenic cultures established from two geographically distinct environments and incubated with different hydrocarbon substrates (added as single hydrocarbons or as mixtures) were subjected to metagenomic and 16S rRNA gene pyrosequencing to test whether these differences affect the genetic potential and composition of the communities. Enrichment of different putative hydrocarbon-degrading bacteria in each culture appeared to be substrate dependent, though all cultures contained both acetate- and H2-utilizing methanogens. Despite differing hydrocarbon substrates and inoculum sources, all three cultures harbored genes for hydrocarbon activation by fumarate addition (bssA, assA, nmsA) and carboxylation (abcA, ancA), along with those for associated downstream pathways (bbs, bcr, bam), though the cultures incubated with hydrocarbon mixtures contained a broader diversity of fumarate addition genes. A comparative metagenomic analysis of the three cultures showed that they were functionally redundant despite their enrichment backgrounds, sharing multiple features associated with syntrophic hydrocarbon conversion to methane. In addition, a comparative analysis of the culture metagenomes with those of 41 environmental samples (containing varying proportions of methanogens) showed that the three cultures were functionally most similar to each other but distinct from other environments, including hydrocarbon-impacted environments (for example, oil sands tailings ponds and oil-affected marine sediments). This study provides a basis for understanding key functions and environmental selection in methanogenic hydrocarbon-associated communities.

  4. Design Factors Affect User Experience for Different Cultural Populations

    ERIC Educational Resources Information Center

    Chu, Sauman

    2016-01-01

    With increasing changes in our demographic populations and new immigrants settling in the US, there is an increasing need for visual communications that address the diversity of our populations. This paper draws from the results of the researcher's several past research and teaching projects that worked with different cultural populations. These…

  5. The ESL Noise Test: Cultural Differences in Affect and Performance.

    ERIC Educational Resources Information Center

    Hansen, Lynne

    This study investigates cultural differences in second language aural comprehension levels among Asians under the constraints of background noise. It is determined that noise is an important component of language proficiency, for it is found where most interactions take place. Students from Hong Kong, Seoul (Korea), Tokyo (Japan), and the South…

  6. What constitutes a good life? Cultural differences in the role of positive and negative affect in subjective well-being.

    PubMed

    Wirtz, Derrick; Chiu, Chi-yue; Diener, Ed; Oishi, Shigehiro

    2009-08-01

    East Asians and Asian Americans report lower levels of subjective well-being than Europeans and European Americans. Three studies found support for the hypothesis that such differences may be due to the psychological meanings Eastern and Western cultures attach to positive and negative affect. Study 1 demonstrated that the desire to repeat a recent vacation was significantly predicted by recalled positive affect-but not recalled negative affect-for European Americans, whereas Asian Americans considered both positive and negative affect. Study 2 replicated this effect in judging satisfaction with a personal friendship. Study 3 linked changes in European Americans' life satisfaction to everyday positive events caused by the self (vs. others) and changes in Japanese life satisfaction to everyday negative events caused by others (vs. the self). Positive affect appears particularly meaningful for European Americans and negative affect for Asian Americans and Japanese when judging a satisfying vacation, friendship, or life.

  7. Agency and facial emotion judgment in context.

    PubMed

    Ito, Kenichi; Masuda, Takahiko; Li, Liman Man Wai

    2013-06-01

    Past research showed that East Asians' belief in holism was expressed as their tendencies to include background facial emotions into the evaluation of target faces more than North Americans. However, this pattern can be interpreted as North Americans' tendency to downplay background facial emotions due to their conceptualization of facial emotion as volitional expression of internal states. Examining this alternative explanation, we investigated whether different types of contextual information produce varying degrees of effect on one's face evaluation across cultures. In three studies, European Canadians and East Asians rated the intensity of target facial emotions surrounded with either affectively salient landscape sceneries or background facial emotions. The results showed that, although affectively salient landscapes influenced the judgment of both cultural groups, only European Canadians downplayed the background facial emotions. The role of agency as differently conceptualized across cultures and multilayered systems of cultural meanings are discussed.

  8. Cultural differences in emotion regulation during self-reflection on negative personal experiences.

    PubMed

    Tsai, William; Lau, Anna S

    2013-01-01

    Reflecting on negative personal experiences has implications for mood that may vary as a function of specific domains (e.g., achievement vs. interpersonal) and cultural orientation (e.g., interdependence vs. independence). This study investigated cultural differences in the social-cognitive and affective processes undertaken as Easterners and Westerners reflected on negative interpersonal and performance experiences. One hundred Asian Americans and 92 European-American college students were randomly assigned to one of three conditions: interpersonal rejection, achievement failure, or a control condition. Results revealed that Asian Americans experienced greater distress than European Americans after self-reflecting over a failed interpersonal experience, suggesting cultural sensitivity in the relational domain. Consistent with theoretical predictions, analysis of the social cognitive and affective processes that participants engaged in during self-reflection provided some evidence that self-enhancement may buffer distress for European Americans, while emotion suppression may be adaptive for Asian Americans.

  9. Designing Transition Programs for Culturally & Linguistically Diverse Students with Disabilities

    ERIC Educational Resources Information Center

    Cote, Debra L.; Jones, Vita L.; Sparks, Shannon L.; Aldridge, Patricia A.

    2012-01-01

    Parents from culturally diverse backgrounds need to feel that they play a vital role in the future success of their sons or daughters with disabilities. Differences in culture and ethnicity can affect families' involvement in transition planning and the goals that they emphasize for their children. Families of diverse backgrounds were surveyed and…

  10. The Impact of National Culture on Informal Learning in the Workplace

    ERIC Educational Resources Information Center

    Kim, Sehoon; McLean, Gary N.

    2014-01-01

    The purpose of this article was to identify how differing cultural factors affect informal learning in the workplace. We have introduced concepts and reviewed studies on informal learning and national culture based on an extensive literature review on the factors influencing informal learning, particularly based on five Hofstede's dimensions of…

  11. Cross-Cultural Transfer in Gesture Frequency in Chinese-English Bilinguals

    ERIC Educational Resources Information Center

    So, Wing Chee

    2010-01-01

    The purpose of this paper is to examine cross-cultural differences in gesture frequency and the extent to which exposure to two cultures would affect the gesture frequency of bilinguals when speaking in both languages. The Chinese-speaking monolinguals from China, English-speaking monolinguals from America, and Chinese-English bilinguals from…

  12. Tissue culture-induced genetic and epigenetic variation in triticale (× Triticosecale spp. Wittmack ex A. Camus 1927) regenerants.

    PubMed

    Machczyńska, Joanna; Zimny, Janusz; Bednarek, Piotr Tomasz

    2015-10-01

    Plant regeneration via in vitro culture can induce genetic and epigenetic variation; however, the extent of such changes in triticale is not yet understood. In the present study, metAFLP, a variation of methylation-sensitive amplified fragment length polymorphism analysis, was used to investigate tissue culture-induced variation in triticale regenerants derived from four distinct genotypes using androgenesis and somatic embryogenesis. The metAFLP technique enabled identification of both sequence and DNA methylation pattern changes in a single experiment. Moreover, it was possible to quantify subtle effects such as sequence variation, demethylation, and de novo methylation, which affected 19, 5.5, 4.5% of sites, respectively. Comparison of variation in different genotypes and with different in vitro regeneration approaches demonstrated that both the culture technique and genetic background of donor plants affected tissue culture-induced variation. The results showed that the metAFLP approach could be used for quantification of tissue culture-induced variation and provided direct evidence that in vitro plant regeneration could cause genetic and epigenetic variation.

  13. Composition of single-step media used for human embryo culture.

    PubMed

    Morbeck, Dean E; Baumann, Nikola A; Oglesbee, Devin

    2017-04-01

    To determine compositions of commercial single-step culture media and test with a murine model whether differences in composition are biologically relevant. Experimental laboratory study. University-based laboratory. Inbred female mice were superovulated and mated with outbred male mice. Amino acid, organic acid, and ions content were determined for single-step culture media: CSC, Global, G-TL, and 1-Step. To determine whether differences in composition of these media are biologically relevant, mouse one-cell embryos were cultured for 96 hours in each culture media at 5% and 20% oxygen in a time-lapse incubator. Compositions of four culture media were analyzed for concentrations of 30 amino acids, organic acids, and ions. Blastocysts at 96 hours of culture and cell cycle timings were calculated, and experiments were repeated in triplicate. Of the more than 30 analytes, concentrations of glucose, lactate, pyruvate, amino acids, phosphate, calcium, and magnesium varied in concentrations. Mouse embryos were differentially affected by oxygen in G-TL and 1-Step. Four single-step culture media have compositions that vary notably in pyruvate, lactate, and amino acids. Blastocyst development was affected by culture media and its interaction with oxygen concentration. Copyright © 2017 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

  14. Choice and dissonance in a European cultural context: the case of Western and Eastern Europeans.

    PubMed

    Kokkoris, Michail D; Kühnen, Ulrich

    2013-01-01

    Prior research demonstrates that members of collectivistic cultures are less likely to reduce cognitive dissonance after making a choice, compared to members of individualistic cultures. This difference has been attributed to different conceptualizations of choice that derive from different self-construals across cultures. In individualistic cultures, choice leads to stronger commitment to the chosen option compared to collectivistic cultures, because it implicates core aspects of the independent self, such as personal preferences. However, this cultural variation in postchoice dissonance has thus far been studied exclusively by comparing East Asians and North Americans. Building on the assumption that this difference is due to different construals of the self, we conducted an experiment with movie choices using the classic free-choice paradigm to examine differences in dissonance reduction between Western and Eastern Europeans, two populations known to differ with respect to interdependence. The results show that Eastern Europeans are less likely than Western Europeans to reduce postchoice dissonance by spreading their alternatives. Our findings speak to the generalizability of the hypothesis that in cultures differing in independence or interdependence people also differ in the way they construe choice, as well as in the way the act of choosing affects their self-concept.

  15. Affective reactions differ between Chinese and American healthy young adults: a cross-cultural study using the international affective picture system.

    PubMed

    Huang, Jinwen; Xu, Dongrong; Peterson, Bradley S; Hu, Jianbo; Cao, Linfeng; Wei, Ning; Zhang, Yingran; Xu, Weijuan; Xu, Yi; Hu, Shaohua

    2015-03-27

    Several cross-cultural studies have suggested that emotions are influenced by the cultural background. Emotional reactions to International Affective Picture System (IAPS) images were compared between Chinese and American young adults. 120 Chinese undergraduates (53 females, 67 males; aged 18-25 years) were enrolled at Zhejiang University, China, and the valence and arousal components of their emotional responses to IAPS images were rated using the Self-Assessment Manikin (SAM) system. Then, valence and arousal scores were compared to those of 100 American undergraduates (50 females, 50 males) of the same age group, enrolled at Florida University and surveyed by Prof. PJ Lang in 2001. Valence scores assigned to 259/816 (31.74%) pictures differed significantly between Chinese and American female participants, while those assigned to 165/816 (20.22%) pictures differed significantly between Chinese and American males (P < 6 × 10(-5)). Of the 816 pictures, the arousal scores assigned to 101/816 (12.38%) pictures differed significantly between Chinese and American female participants; these scores significantly differed in 130/816 (15.93%) pictures between Chinese and American males (P < 6 × 10(-5)). Valence scores for pictures in the Erotic category differed significantly between Chinese and American females (P < 6 × 10(-5)). There were no significant differences in valence scores for the remaining eight categories studied between participants from the two countries, whether female or male. The IAPS norms require a modification for their appropriate application in Asian cultures.

  16. Institutional Guidance of Affective Bonding: Moral Values Development in Brazilian Military Education.

    PubMed

    Wortmeyer, Daniela Schmitz; Branco, Angela Uchoa

    2016-09-01

    In this article, our aim is to analyze institutional practices guided to promote the development of moral values within the context of military education of Brazilian Army combatant commissioned officers. From a cultural psychological approach, we discuss how social guidance within military culture operates at different levels of the affective-semiotic regulation of individuals, structuring complex experiences that give rise to hypergeneralized meaning fields regarding morality and military values. For this goal, we first introduce some theoretical topics related to values development, emphasizing their affective roots and role in the emergence, maintenance, amplification and attenuation of all relations between the person and the environment. Following a brief discussion on how social institutions try to promote changes in personal values, we provide an overview of values present in the military culture and socialization. Finally, the text focuses on the education of Brazilian Army combatant commissioned officers, describing how practices related to different levels of affective-semiotic experience combine in order to promote the internalization and externalization of specific moral values. We conclude suggesting issues for future investigation.

  17. Optimum culture in the cockpit

    NASA Technical Reports Server (NTRS)

    Yamamori, Hisaaki

    1987-01-01

    Even with the same program and objectives, if the culture is different, there will be different approaches to the goal of flight safety. However, the cockpit environment is culture-free so it is not as important to think of a person's cultural background as it is to think of the approach to the goal of ultimate safety. Crew members can look at their individual safety goals and compare them to their own performance to see if their behavior matches their own safety goals. The cockpit environment must be culture-free in order to obtain the ultimate safety goal. One must first realize how their culture affects their behavior before they can begin to change their attitude and actions in the cockpit.

  18. Two Sides of Emotion: Exploring Positivity and Negativity in Six Basic Emotions across Cultures.

    PubMed

    An, Sieun; Ji, Li-Jun; Marks, Michael; Zhang, Zhiyong

    2017-01-01

    We employ a novel paradigm to test whether six basic emotions (sadness, fear, disgust, anger, surprise, and happiness; Ekman, 1992) contain both negativity and positivity, as opposed to consisting of a single continuum between negative and positive. We examined the perceived negativity and positivity of these emotions in terms of their affective and cognitive components among Korean, Chinese, Canadian, and American students. Assessing each emotion at the cognitive and affective levels cross-culturally provides a fairly comprehensive picture of the positivity and negativity of emotions. Affective components were rated as more divergent than cognitive components. Cross-culturally, Americans and Canadians gave higher valence ratings to the salient valence of each emotion, and lower ratings to the non-salient valence of an emotion, compared to Chinese and Koreans. The results suggest that emotions encompass both positivity and negativity, and there were cross-cultural differences in reported emotions. This paradigm complements existing emotion theories, building on past research and allowing for more parsimonious explanations of cross-cultural research on emotion.

  19. A Crosscultural Analysis of Argumentative Strategies in Student Essays.

    ERIC Educational Resources Information Center

    Kamimura, Taeko; Oi, Kyoko

    A study of essays on a single topic (capital punishment) written by 22 American high school students and 30 second-year Japanese college students investigated: cultural differences in organizational patterns in argumentative essays; comparative use of rational and affective appeals; differences in content of rational and affective appeals;…

  20. Cultural Factors Affecting the Differential Performance of Israeli and Palestinian Children on the Loewenstein Occupational Therapy Cognitive Assessment

    ERIC Educational Resources Information Center

    Josman, Naomi; Abdallah, Taisir M.; Engel-Yeger, Batya

    2010-01-01

    Cognitive performance is essential for children's functioning and may also predict school readiness. The suitability of Western standardized assessments for cognitive performance among children from different cultures needs to be elaborated. This study referred to the existence of differences in cognitive performance between and within children…

  1. The Relationship between Degree of Acculturation and Adjustment Difficulties among Korean Immigrants Living in a Western Society

    ERIC Educational Resources Information Center

    Shim, Young R.; Schwartz, Robert C.

    2007-01-01

    When immigrating to a new society individuals typically have different behavioural patterns and cultural values than the host country. These differences often lead to acculturation difficulties among immigrants. The purpose of this study was to examine how behavioural and value acculturation affects cultural adjustment problems among Korean…

  2. Effects of Working Couple's Retirement Sequence on Satisfaction in Patriarchal Culture Country: Probing on Gender Difference.

    PubMed

    Lee, Ayoung; Cho, Joonmo

    2017-01-01

    We examined the effects of the differences in the retirement sequence (i.e., who retires first between spouses) on satisfaction in Korea of patriarchal culture. Our empirical study demonstrates that households where men retired first had a much lower satisfaction than households where women retired first. In addition, men were found to show lower satisfaction than wives in both households where women retire first and the households where men retire first. Retirement sequence affecting their satisfaction at the point when only one of the spouses is retired continues to affect their satisfaction after both of them are retired. This means that the difference in the couple's retirement sequence has an ongoing effect on their later happiness. The analysis of the effect of a couple's retirement sequence on the satisfaction in their old life may be useful for improving an individual and couples' quality of life in countries with similar cultures.

  3. Organizational Culture and Climate and Mental Health Provider Attitudes Toward Evidence-Based Practice

    PubMed Central

    Aarons, Gregory A.; Sawitzky, Angelina C.

    2006-01-01

    Mental health provider attitudes toward adopting evidence-based practice (EBP) are associated with organizational context and provider individual differences. Organizational culture and climate are contextual factors that can affect staff acceptance of innovation. This study examined the association of organizational culture and climate with attitudes toward adopting EBP. Participants were 301 public sector mental health service providers from 49 programs providing mental health services for youths and families. Correlation analyses and multilevel hierarchical regressions, controlling for effects of provider characteristics, showed that constructive culture was associated with more positive attitudes toward adoption of EBP and poor organizational climates with perceived divergence of usual practice and EBP. Behavioral health organizations may benefit from consideration of how culture and climate affect staff attitudes toward change in practice. PMID:17183411

  4. Organizational Culture and Climate and Mental Health Provider Attitudes Toward Evidence-Based Practice.

    PubMed

    Aarons, Gregory A; Sawitzky, Angelina C

    2006-02-01

    Mental health provider attitudes toward adopting evidence-based practice (EBP) are associated with organizational context and provider individual differences. Organizational culture and climate are contextual factors that can affect staff acceptance of innovation. This study examined the association of organizational culture and climate with attitudes toward adopting EBP. Participants were 301 public sector mental health service providers from 49 programs providing mental health services for youths and families. Correlation analyses and multilevel hierarchical regressions, controlling for effects of provider characteristics, showed that constructive culture was associated with more positive attitudes toward adoption of EBP and poor organizational climates with perceived divergence of usual practice and EBP. Behavioral health organizations may benefit from consideration of how culture and climate affect staff attitudes toward change in practice.

  5. Foreign Culture Awareness Needs of Saudi English Language Majors at Buraydah Community College

    ERIC Educational Resources Information Center

    Alsamani, Abdul-Aziz Saleh

    2014-01-01

    Although many EFL learners have a command of internalized foreign language knowledge, they may have difficulty using this knowledge in different contexts. This is due to many interacting factors affecting their performance, mainly lack of target culture awareness. This study intended to identify the cultural aspects suitable to be integrated into…

  6. An Investigation of Cultural Differences in Perceived Speaker Effectiveness.

    ERIC Educational Resources Information Center

    Masterson, John T.; Watson, Norman H.

    Culture is a powerful force that may affect the reception and acceptance of communication. To determine if culture has an effect on perceived speaker effectiveness, students in one introductory speech communication class in a Florida university and a variety of courses in a university in the Bahamas, were given an 80-item questionnaire that was…

  7. Is it culture or is it language? Examination of language effects in cross-cultural research on categorization.

    PubMed

    Ji, Li-Jun; Zhang, Zhiyong; Nisbett, Richard E

    2004-07-01

    Differences in reasoning styles between Chinese and European Americans held even when controlling for the language of testing. Bilingual Chinese organized objects in a more relational and less categorical way than European Americans, whether tested in English or in Chinese. Thus, culture affects categorization independent of the testing language. Nevertheless, language affected some Chinese bilinguals' categorization. The responses of Chinese from the Mainland and Taiwan were more relational when tested in Chinese than when tested in English. Responses of Chinese from Hong Kong and Singapore were equally relational when tested in Chinese and in English. Age and context of learning English are discussed to explain the differential language effects among different Chinese groups. Theoretical and methodological implications are discussed. Copyright 2004 American Psychological Association

  8. Age affects not only metabolome but also metal toxicity in Scenedesmus quadricauda cultures.

    PubMed

    Kováčik, Jozef; Klejdus, Bořivoj; Babula, Petr; Hedbavny, Josef

    2016-04-05

    Responses of Scenedesmus quadricauda grown in vitro and differing in age (old culture-13 months, young culture-1 month) to short-term cadmium (Cd) or nickel (Ni) excess (24h) were compared. Higher age of the culture led to lower amount of chlorophylls, ascorbic acid and glutathione but higher signal of ROS. Surprisingly, sucrose was detected using DART-Orbitrap MS in both old and young culture and subsequent quantification confirmed its higher amount (ca. 3-times) in the old culture. Cd affected viability and ROS amount more negatively than Ni that could arise from excessive Cd uptake which was also higher in all treatments than in respective Ni counterparts. Surprisingly, nitric oxide was not extensively different in response to age or metals. Strong induction of phytochelatin 2 is certainly Cd-specific response while Ni also elevated ascorbate content. Krebs cycle acids were more accumulated in the young culture but they were rather elevated in the old culture (citric acid under Ni excess). We conclude that organic solid 'Milieu Bristol' medium we tested is suitable for long-term storage of unicellular green algae (also successfully tested for Coccomyxa sp. and Parachlorella sp.) and the impact of age on metal uptake may be useful for bioremediation purposes. Copyright © 2015 Elsevier B.V. All rights reserved.

  9. Wanting to Maximize the Positive and Minimize the Negative: Implications for Mixed Affective Experience in American and Chinese Contexts

    PubMed Central

    Sims, Tamara; Tsai, Jeanne L.; Jiang, Da; Wang, Yaheng; Fung, Helene H.; Zhang, Xiulan

    2016-01-01

    Previous studies have demonstrated that European Americans have fewer mixed affective experiences (i.e., are less likely to experience the bad with the good) compared to Chinese. In this paper, we argue that these cultural differences are due to “ideal affect,” or how people ideally want to feel. Specifically, we predict that people from individualistic cultures want to maximize positive and minimize negative affect more than people from collectivistic cultures, and as a result, they are less likely to actually experience mixed emotions (reflected by a more negative within-person correlation between actual positive and negative affect). We find support for this prediction in two experience sampling studies conducted in the U.S. and China (Studies 1 and 2). In addition, we demonstrate that ideal affect is a distinct construct from dialectical view of the self, which has also been related to mixed affective experience (Study 3). Finally, in Study 4, we demonstrate that experimentally manipulating the desire to maximize the positive and minimize the negative alters participants' actual experience of mixed emotions during a pleasant (but not unpleasant or combined pleasant and unpleasant) television clip in the U.S. and Hong Kong. Together, these findings suggest that across cultures, how people want to feel shapes how they actually feel, particularly people's mixed affective experience. PMID:26121525

  10. Wanting to maximize the positive and minimize the negative: implications for mixed affective experience in American and Chinese contexts.

    PubMed

    Sims, Tamara; Tsai, Jeanne L; Jiang, Da; Wang, Yaheng; Fung, Helene H; Zhang, Xiulan

    2015-08-01

    Previous studies have demonstrated that European Americans have fewer mixed affective experiences (i.e., are less likely to experience the bad with the good) compared with Chinese. In this article, we argue that these cultural differences are due to "ideal affect," or how people ideally want to feel. Specifically, we predict that people from individualistic cultures want to maximize positive and minimize negative affect more than people from collectivistic cultures, and as a result, they are less likely to actually experience mixed emotions (reflected by a more negative within-person correlation between actual positive and negative affect). We find support for this prediction in 2 experience sampling studies conducted in the United States and China (Studies 1 and 2). In addition, we demonstrate that ideal affect is a distinct construct from dialectical view of the self, which has also been related to mixed affective experience (Study 3). Finally, in Study 4, we demonstrate that experimentally manipulating the desire to maximize the positive and minimize the negative alters participants' actual experience of mixed emotions during a pleasant (but not unpleasant or combined pleasant and unpleasant) TV clip in the United States and Hong Kong. Together, these findings suggest that across cultures, how people want to feel shapes how they actually feel, particularly people's experiences of mixed affect. (c) 2015 APA, all rights reserved.

  11. Cross-Cultural Differences in Beliefs and Practices that Affect the Language Spoken to Children: Mothers with Indian and Western Heritage

    ERIC Educational Resources Information Center

    Simmons, Noreen; Johnston, Judith

    2007-01-01

    Background: Speech-language pathologists often advise families about interaction patterns that will facilitate language learning. This advice is typically based on research with North American families of European heritage and may not be culturally suited for non-Western families. Aims: The goal of the project was to identify differences in the…

  12. On "feeling right" in cultural contexts: how person-culture match affects self-esteem and subjective well-being.

    PubMed

    Fulmer, C Ashley; Gelfand, Michele J; Kruglanski, Arie W; Kim-Prieto, Chu; Diener, Ed; Pierro, Antonio; Higgins, E Tory

    2010-11-01

    Whether one is in one's native culture or abroad, one's personality can differ markedly from the personalities of the majority, thus failing to match the "cultural norm." Our studies examined how the interaction of individual- and cultural-level personality affects people's self-esteem and well-being. We propose a person-culture match hypothesis that predicts that when a person's personality matches the prevalent personalities of other people in a culture, culture functions as an important amplifier of the positive effect of personality on self-esteem and subjective well-being at the individual level. Across two studies, using data from more than 7,000 individuals from 28 societies, multilevel random-coefficient analyses showed that when a relation between a given personality trait and well-being or self-esteem exists at the individual level, the relation is stronger in cultures characterized by high levels of that personality dimension. Results were replicated across extraversion, promotion focus, and locomotive regulatory mode. Our research has practical implications for the well-being of both cultural natives and migrants.

  13. Cultural and language differences in voice quality perception: a preliminary investigation using synthesized signals.

    PubMed

    Yiu, Edwin M-L; Murdoch, Bruce; Hird, Kathryn; Lau, Polly; Ho, Elaine Mandy

    2008-01-01

    Perceptual voice evaluation is a common clinical tool. However, to date, there is no consensus yet as to which common quality should be measured. Some available evidence shows that voice quality is a language-specific property which may be different across different languages. The familiarity of a language may affect the perception and reliability in rating voice quality. The present study set out to investigate the effects of listeners' cultural and language backgrounds on the perception of voice qualities. Forty speech pathology students from Australia and Hong Kong were asked to rate the breathy and rough qualities of synthesized voice signals in Cantonese and English. Results showed that the English stimulus sets as a whole were rated less severely than the Cantonese stimuli by both groups of listeners. In addition, the male Cantonese and English breathy stimuli were rated differently by the Australian and Hong Kong listeners. These results provided some evidence to support the claim that cultural and language backgrounds of the listeners would affect the perception for some voice quality types. Thus, the cultural and language backgrounds of judges should be taken into consideration in clinical voice evaluation. 2008 S. Karger AG, Basel.

  14. Pb and Cd binding to natural freshwater biofilms developed at different pH: the important role of culture pH.

    PubMed

    Hua, Xiuyi; Dong, Deming; Ding, Xiaoou; Yang, Fan; Jiang, Xu; Guo, Zhiyong

    2013-01-01

    The effects of solution pH on adsorption of trace metals to different types of natural aquatic solid materials have been studied extensively, but few studies have been carried out to investigate the effect of pH at which the solid materials were formed on the adsorption. The purpose of present study is to examine this effect of culture pH on metal adsorption to natural freshwater biofilms. The adsorption of Pb and Cd to biofilms which were developed at different culture pH values (ranging from 6.5 to 9.0) was measured at the same adsorption pH value (6.5). The culture pH had considerable effects on both composition and metal adsorption ability of the biofilms. Higher culture pH usually promoted the accumulation of organic material and Fe oxides in the biofilms. The culture pH also affected the quantity and species of algae in the biofilms. The adsorption of Pb and Cd to the biofilms generally increased with the increase of culture pH. This increase was minor at lower pH range and significant at higher pH range and was more remarkable for Cd adsorption than for Pb adsorption. The notable contribution of organic material to the adsorption at higher culture pH values was also observed. The profound impacts of culture pH on adsorption behavior of biofilms mainly resulted from the variation of total contents of the biofilm components and were also affected by the alteration of composition and properties of the components.

  15. Even subtle cultural differences affect face tuning.

    PubMed

    Pavlova, Marina A; Heiz, Julie; Sokolov, Alexander N; Fallgatter, Andreas J; Barisnikov, Koviljka

    2018-01-01

    Culture shapes social cognition in many ways. Yet cultural impact on face tuning remains largely unclear. Here typically developing females and males from the French-speaking part of Switzerland were presented with a set of Arcimboldo-like Face-n-Food images composed of food ingredients and in different degree resembling a face. The outcome had been compared with previous findings obtained in young adults of the South-West Germany. In that study, males exhibit higher thresholds for face tuning on the Face-n-Food task than females. In Swiss participants, no gender differences exist in face tuning. Strikingly, males from the French-speaking part of Switzerland possess higher sensitivity to faces than their German peers, whereas no difference in face tuning occurs between females. The outcome indicates that even relatively subtle cultural differences as well as culture by gender interaction can modulate social cognition. Clarification of the nature of cultural impact on face tuning as well as social cognition at large is of substantial value for understanding a wide range of neuropsychiatric and neurodevelopmental conditions.

  16. Health-related quality of life of irritable bowel syndrome patients in different cultural settings.

    PubMed

    Faresjö, Ashild; Anastasiou, Foteini; Lionis, Christos; Johansson, Saga; Wallander, Mari-Ann; Faresjö, Tomas

    2006-03-27

    Persons with Irritable bowel syndrome (IBS) are seriously affected in their everyday life. The effect across different cultural settings of IBS on their quality of life has been little studied. The aim was to compare health-related quality of life (HRQOL) of individuals suffering from IBS in two different cultural settings; Crete, Greece and Linköping, Sweden. This study is a sex and age-matched case-control study, with n = 30 Cretan IBS cases and n = 90 Swedish IBS cases and a Swedish control group (n = 300) randomly selected from the general population. Health-related quality of life, measured by SF-36 and demographics, life style indicators and co-morbidity, was measured. Cretan IBS cases reported lower HRQOL on most dimensions of SF-36 in comparison to the Swedish IBS cases. Significant differences were found for the dimensions mental health (p < 0.0001) and general health (p = 0.05) even after adjustments for educational level and co-morbidity. Women from Crete with IBS scored especially low on the dimensions general health (p = 0.009) and mental health (p < 0.0001) in comparison with Swedish women with IBS. The IBS cases, from both sites, reported significantly lower scores on all HRQOL dimensions in comparison with the Swedish control group. The results from this study tentatively support that the claim that similar individuals having the same disease, e.g. IBS, but living in different cultural environments could perceive their disease differently and that the disease might affect their everyday life and quality of life in a different way. The Cretan population, and especially women, are more seriously affected mentally by their disease than Swedish IBS cases. Coping with IBS in everyday life might be more problematic in the Cretan environment than in the Swedish setting.

  17. Cultural values and the prevalence of mental disorders in 25 countries: A secondary data analysis.

    PubMed

    Heim, Eva; Wegmann, Iris; Maercker, Andreas

    2017-09-01

    The prevalence of common mental disorders (CMDs, i.e., depression and anxiety) worldwide is substantial, and prevalence rates are higher in high-income than in low- and middle-income countries. This difference might reflect both underlying prevalence rates as well as the measurement model used in cross-national epidemiological studies. Schwartz' cultural values provide a meaningful taxonomy to describe 'culture' and to examine how culture affects both the aetiology and phenomenology of CMDs. The present study examines to what extent Schwartz' cultural values correlate with prevalence rates of CMDs at the country-level. Twenty-five countries were included in this study. Countries were included if data on cultural values and lifetime prevalence rates, from either the World Mental Health Surveys or the Global Burden of Disease Study, were available for at least one CMD. Spearman rank correlations were calculated between prevalence rates and cultural values, controlling for gross national income (GNI) per capita. Affective disorders correlated with cultural values, after controlling for GNI. For anxiety disorders, correlations were lower but still offered meaningful insights. Correlations followed the circular structure of values, meaning that the strength of relationship decreased and increased again when moving around the circle: the strongest positive correlations were found with egalitarianism, and the strongest negative correlations with hierarchy and mastery. The autonomy-embeddedness dimension correlated weakly with the prevalence of CMDs. Diverging prevalence rates between high-income countries and low- and middle-income countries are associated with differences in cultural values. Values might not only relate to the aetiology of mental disorders, but most possibly affect the way in which psychological distress is expressed. As an example, in societies with a strong focus on embeddedness, the fear of stigma might be more pronounced. Cultural values offer a middle ground between culturally specific (i.e., emic) and universalist (i.e., etic) research. Copyright © 2017 Elsevier Ltd. All rights reserved.

  18. Early competitive effects on growth of loblolly pine grown in co-culture with switchgrass

    Treesearch

    Kurt J. Krapfl; Scott D. Roberts; Randall J. Rosseau; Jeff A. Hatten

    2015-01-01

    This study: (1) examined competitive interactions between switchgrass and loblolly pine grown in co-culture, and (2) assessed early growth rates of loblolly pine as affected by differing switchgrass competition treatments. Co-cultures were established and monitored on two Upper Coastal Plain sites for 2 years. The Pontotoc site has a history of agricultural use with...

  19. Study of American and Chinese family members' evaluations on institutionalized care for their older parents: potential development in the future.

    PubMed

    Li, Yushi; Buechel, Annie

    2007-01-01

    The evaluations on institutionalized care facilities from family members, after their loved ones moved into such services, are very different from culture to culture, family to family and person to person. According to a recent survey in the United States and China, it is found that different cultures and the different health conditions of the residents strongly influence family member's viewpoints on institutionalized care services. It is also found that the availability of the institutionalized care facilities plays a significant role, which strongly affects family members' evaluations on nursing home services.

  20. Skin antiseptics in venous puncture site disinfection for preventing blood culture contamination: A Bayesian network meta-analysis of randomized controlled trials.

    PubMed

    Liu, Wenjie; Duan, Yuchen; Cui, Wenyao; Li, Li; Wang, Xia; Dai, Heling; You, Chao; Chen, Maojun

    2016-07-01

    To compare the efficacy of several antiseptics in decreasing the blood culture contamination rate. Network meta-analysis. Electronic searches of PubMed and Embase were conducted up to November 2015. Only randomized controlled trials or quasi-randomized controlled trials were eligible. We applied no language restriction. A comprehensive review of articles in the reference lists was also accomplished for possible relevant studies. Relevant studies evaluating efficacy of different antiseptics in venous puncture site for decreasing the blood culture contamination rate were included. The data were extracted from the included randomized controlled trials by two authors independently. The risk of bias was evaluated using Detsky scale by two authors independently. We used WinBUGS1.43 software and statistic model described by Chaimani to perform this network meta-analysis. Then graphs of statistical results of WinBUGS1.43 software were generated using 'networkplot', 'ifplot', 'netfunnel' and 'sucra' procedure by STATA13.0. Odds ratio and 95% confidence intervals were assessed for dichotomous data. A probability of p less than 0.05 was considered to be statistically significant. Compared with ordinary meta-analyses, this network meta-analysis offered hierarchies for the efficacy of different antiseptics in decreasing the blood culture contamination rate. Seven randomized controlled trials involving 34,408 blood samples were eligible for the meta-analysis. No significant difference was found in blood culture contamination rate among different antiseptics. No significant difference was found between non-alcoholic antiseptics and alcoholic antiseptics, alcoholic chlorhexidine and povidone iodine, chlorhexidine and iodine compounds, povidone iodine and iodine tincture in this aspect, respectively. Different antiseptics may not affect the blood culture contamination rate. Different intervals between the skin disinfection and the venous puncture, the different settings (emergency room, medical wards, and intensive care units) and the performance of the phlebotomy may affect the blood culture contamination rate. Copyright © 2016 Elsevier Ltd. All rights reserved.

  1. A Multinational Study Examining the Cross Cultural Differences in Reported Symptoms of Autism Spectrum Disorders: Israel, South Korea, the United Kingdom, and the United States of America

    ERIC Educational Resources Information Center

    Matson, Johnny L.; Worley, Julie A.; Fodstad, Jill C.; Chung, Kyong-Mee; Suh, Dongsoo; Jhin, Hea Kyung; Ben-Itzchak, Esther; Zachor, Ditza A.; Furniss, Frederick

    2011-01-01

    Purportedly, there is a worldwide acceptance of diagnostic criteria for Autism Spectrum Disorders (ASD); however, cultural differences in regards to what is considered normal development may affect the diagnosis despite the biological basis for the condition. The aim of the current study was to examine the differences in reports of symptoms of ASD…

  2. Exploring the influence of cultural orientations on assessment of communication behaviours during patient-practitioner interactions.

    PubMed

    Wilby, Kyle J; Govaerts, Marjan J B; Austin, Zubin; Dolmans, Diana H J M

    2017-03-21

    Research has shown that patients' and practitioners' cultural orientations affect communication behaviors and interpretations in cross-cultural patient-practitioner interactions. Little is known about the effect of cultural orientations on assessment of communication behaviors in cross-cultural educational settings. The purpose of this study is to explore cultural orientation as a potential source of assessor idiosyncrasy or between-assessor variability in assessment of communication skills. More specifically, we explored if and how (expert) assessors' valuing of communication behaviours aligned with their cultural orientations (power-distance, masculinity-femininity, uncertainty avoidance, and individualism-collectivism). Twenty-five pharmacist-assessors watched 3 videotaped scenarios (patient-pharmacist interactions) and ranked each on a 5-point global rating scale. Videotaped scenarios demonstrated combinations of well-portrayed and borderline examples of instrumental and affective communication behaviours. We used stimulated recall and verbal protocol analysis to investigate assessors' interpretations and evaluations of communication behaviours. Uttered assessments of communication behaviours were coded as instrumental (task-oriented) or affective (socioemotional) and either positive or negative. Cultural orientations were measured using the Individual Cultural Values Scale. Correlations between cultural orientations and global scores, and frequencies of positive, negative, and total utterances of instrumental and affective behaviours were determined. Correlations were found to be scenario specific. In videos with poor or good performance, no differences were found across cultural orientations. When borderline performance was demonstrated, high power-distance and masculinity were significantly associated with higher global ratings (r = .445, and .537 respectively, p < 0.05) as well as with fewer negative utterances regarding instrumental (task focused) behaviours (r = -.533 and - .529, respectively). Higher masculinity scores were furthermore associated with positive utterances of affective (socioemotional) behaviours (r = .441). Our findings thus confirm cultural orientation as a source of assessor idiosyncrasy and meaningful variations in interpretation of communication behaviours. Interestingly, expert assessors generally agreed on scenarios of good or poor performances but borderline performance was influenced by cultural orientation. Contrary to current practices of assessor and assessment instrument standardization, findings support the use of multiple assessors for patient-practitioner interactions and development of qualitative assessment tools to capture these varying, yet valid, interpretations of performance.

  3. What Constitutes a Good Life? Cultural Differences in the Role of Positive and Negative Affect in Subjective Well-Being

    PubMed Central

    Wirtz, Derrick; Chiu, Chi-yue; Diener, Ed; Oishi, Shigehiro

    2009-01-01

    East Asians and Asian Americans report lower levels of subjective well-being than Europeans and European Americans. Three studies found support for the hypothesis that such differences may be due to the psychological meanings Eastern and Western cultures attach to positive and negative affect. Study 1 demonstrated that the desire to repeat a recent vacation was significantly predicted by recalled positive affect—but not recalled negative affect—for European Americans, whereas Asian Americans considered both positive and negative affect. Study 2 replicated this effect in judging satisfaction with a personal friendship. Study 3 linked changes in European Americans’ life satisfaction to everyday positive events caused by the self (vs. others) and changes in Japanese life satisfaction to everyday negative events caused by others (vs. the self). Positive affect appears particularly meaningful for European Americans and negative affect for Asian Americans and Japanese when judging a satisfying vacation, friendship, or life. PMID:19558439

  4. A cross-cultural perspective on irritable bowel syndrome.

    PubMed

    Gerson, Charles D; Gerson, Mary-Joan

    2010-01-01

    Irritable bowel syndrome is a functional gastrointestinal illness, defined by symptoms. Irritable bowel syndrome has been described as a biopsychosocial condition, in which colonic dysfunction is affected by psychological and social factors. As a result of this unusual constellation, irritable bowel syndrome may be subject to cultural variables that differ in different parts of the globe. In this article, we describe some of the ways in which irritable bowel syndrome may be experienced differently, depending on local belief systems, psychological pressures, acceptance or resistance to a mind-body paradigm, and breakdown in support or relationship structure. Examples are given in which irritable bowel syndrome investigators from countries around the world describe various aspects of the syndrome that may affect the illness experience of their patients. We describe our own research studies that have demonstrated possible adverse effects on disease severity from relationship conflict, attribution of symptoms to physical rather than emotional cause, and the belief that irritable bowel syndrome is enduring and mysterious. Also described is our finding that symptom patterns may differ significantly between different geographic locations. Finally, we discuss the importance of "cultural competence" on the part of healthcare professionals in regard to caring for patients of diverse cultural backgrounds. © 2010 Mount Sinai School of Medicine.

  5. Is Perceptual Priming Affected by Culture? A Study With German Middle-Class and Cameroonian Nso Farmer Children.

    PubMed

    Vöhringer, Isabel Aline; Poloczek, Sonja; Graf, Frauke; Lamm, Bettina; Teiser, Johanna; Fassbender, Ina; Freitag, Claudia; Suhrke, Janina; Teubert, Manuel; Keller, Heidi; Lohaus, Arnold; Schwarzer, Gudrun; Knopf, Monika

    2015-01-01

    The authors explored priming in children from different cultural environments with the aim to provide further evidence for the robustness of the priming effect. Perceptual priming was assessed by a picture fragment completion task in 3-year-old German middle-class and Cameroonian Nso farmer children. As expected, 3-year-olds from both highly diverging cultural contexts under study showed a priming effect, and, moreover, the effect was of comparable size in both cultural contexts. Hence, the children profited similarly from priming, which was supported by the nonsignificant interaction between cultural background and identification performance as well as the analysis of absolute difference scores. However, a culture-specific difference regarding the level of picture identification was found in that German middle-class children identified target as well as control pictures with less perceptual information than children in the Nso sample. Explanations for the cross-cultural demonstration of the priming effect as well as for the culturally diverging levels on which priming occurs are discussed.

  6. Multicultural Nursing: Providing Better Employee Care.

    PubMed

    Rittle, Chad

    2015-12-01

    Living in an increasingly multicultural society, nurses are regularly required to care for employees from a variety of cultural backgrounds. An awareness of cultural differences focuses occupational health nurses on those differences and results in better employee care. This article explores the concept of culturally competent employee care, some of the non-verbal communication cues among cultural groups, models associated with completing a cultural assessment, and how health disparities in the workplace can affect delivery of employee care. Self-evaluation of the occupational health nurse for personal preferences and biases is also discussed. Development of cultural competency is a process, and occupational health nurses must develop these skills. By developing cultural competence, occupational health nurses can conduct complete cultural assessments, facilitate better communication with employees from a variety of cultural backgrounds, and improve employee health and compliance with care regimens. Tips and guidelines for facilitating communication between occupational health nurses and employees are also provided. © 2015 The Author(s).

  7. Evaluation of the udder health status in subclinical mastitis affected dairy cows through bacteriological culture, somatic cell count and thermographic imaging.

    PubMed

    Bortolami, A; Fiore, E; Gianesella, M; Corrò, M; Catania, S; Morgante, M

    2015-01-01

    Subclinical mastitis in dairy cows is a big economic loss for farmers. The monitoring of subclinical mastitis is usually performed through Somatic Cell Count (SCC) in farm but there is the need of new diagnostic systems able to quickly identify cows affected by subclinical infections of the udder. The aim of this study was to evaluate the potential application of thermographic imaging compared to SCC and bacteriological culture for infection detection in cow affected by subclinical mastitis and possibly to discriminate between different pathogens. In this study we evaluated the udder health status of 98 Holstein Friesian dairy cows with high SCC in 4 farms. From each cow a sample of milk was collected from all the functional quarters and submitted to bacteriological culture, SCC and Mycoplasma spp. culture. A thermographic image was taken from each functional udder quarter and nipple. Pearson's correlations and Analysis of Variance were performed in order to evaluate the different diagnostic techniques. The most frequent pathogen isolated was Staphylococcus aureus followed by Coagulase Negative Staphylococci (CNS), Streptococcus uberis, Streptococcus agalactiae and others. The Somatic Cell Score (SCS) was able to discriminate (p<0.05) cows positive for a pathogen from cows negative at the bacteriological culture except for cows with infection caused by CNS. Infrared thermography was correlated to SCS (p<0.05) but was not able to discriminate between positive and negative cows. Thermographic imaging seems to be promising in evaluating the inflammation status of cows affected by subclinical mastitis but seems to have a poor diagnostic value.

  8. Dynamic cultural influences on neural representations of the self.

    PubMed

    Chiao, Joan Y; Harada, Tokiko; Komeda, Hidetsugu; Li, Zhang; Mano, Yoko; Saito, Daisuke; Parrish, Todd B; Sadato, Norihiro; Iidaka, Tetsuya

    2010-01-01

    People living in multicultural environments often encounter situations which require them to acquire different cultural schemas and to switch between these cultural schemas depending on their immediate sociocultural context. Prior behavioral studies show that priming cultural schemas reliably impacts mental processes and behavior underlying self-concept. However, less well understood is whether or not cultural priming affects neurobiological mechanisms underlying the self. Here we examined whether priming cultural values of individualism and collectivism in bicultural individuals affects neural activity in cortical midline structures underlying self-relevant processes using functional magnetic resonance imaging. Biculturals primed with individualistic values showed increased activation within medial prefrontal cortex (MPFC) and posterior cingulate cortex (PCC) during general relative to contextual self-judgments, whereas biculturals primed with collectivistic values showed increased response within MPFC and PCC during contextual relative to general self-judgments. Moreover, degree of cultural priming was positively correlated with degree of MPFC and PCC activity during culturally congruent self-judgments. These findings illustrate the dynamic influence of culture on neural representations underlying the self and, more broadly, suggest a neurobiological basis by which people acculturate to novel environments.

  9. The effects of the gender-culture interaction on self-reports of depressive symptoms: cross-cultural study among Egyptians and Canadians.

    PubMed

    Huang, Vivian; Beshai, Shadi; Yu, Mabel

    2016-01-01

    Research in depression has revealed differences in the way depressed individuals across cultures report their symptoms. This literature also points to possible differences in symptom reporting patterns between men and women. Using data from a larger dataset (Beshai et al. 2016), the current study examined whether non-depressed and depressed Egyptian and Canadian men and women differed in their self-report of the various domains of the Beck Depression Inventory -II (BDI-II). We recruited a total of 131 depressed and non-depressed participants from both Egypt ( n = 29 depressed; n = 29 non-depressed) and Canada ( n = 35 depressed; n = 38 non-depressed). Depression status was ascertained using a structured interview. All participants were asked to complete the BDI-II along with other self-report measures of depression. BDI-II items were divided into two subscales in accordance with Dozois, Dobson & Ahnberg (1998) factor analysis: cognitive-affective and somatic-vegetative subscales. We found a significant three-way interaction effect on the cognitive-affective ( F (1,121) = 9.51, p = .003) and main effect of depression status on somatic-vegetative subscales ( F (1,121) = 42.80, p < .001). Post hoc analyses revealed that depressed Egyptian men reported lower scores on the cognitive-affective subscale of the BDI-II compared to their depressed Canadian male counterparts. These results suggest that males across cultures may differentially report cognitive symptoms of depression. These results also suggest that clinicians and clinical scientists need to further examine the interaction effect of culture and gender when investigating self-reported symptoms of depression.

  10. Cultural and communicative memories: contrasting Argentina's 1976 coup d'état and the 2001 economic-political-social crisis.

    PubMed

    Muller, Felipe; Bermejo, Federico; Hirst, William

    2018-08-01

    Studies on collective memory have recently addressed the distinction between cultural and communicative memory as a way to understand how the source of a memory affects its structure or form. When a groups' memory is mediated by memorials, documentaries or any other cultural artifacts, collective memory is shaped by cultural memory. When it is based mostly in communication with other people, its source is communicative memory. We address this distinction by studying two recent events in Argentinean history: the 2001 economic-political-social crisis (communicative memory) and the 1976 coup (cultural memory). We also examine the political ideology and the type of memory involved in collective memory. The memory of the studied events may occur during the lifetime of the rememberer (Lived Memory) or refer to distant events (Distant Memory). 100 participants responded to a Free Recall task about the events of 2001 in Argentina. Narrative analysis allowed comparing these recalls with our 1976 study. Results show: 1) Cultural memories are more contextualised, more impersonal and less affective. 2) Communicative memories are more personal and affective. Study shows how collective memory form changes when it has a different prevalent source.

  11. Two Sides of Emotion: Exploring Positivity and Negativity in Six Basic Emotions across Cultures

    PubMed Central

    An, Sieun; Ji, Li-Jun; Marks, Michael; Zhang, Zhiyong

    2017-01-01

    We employ a novel paradigm to test whether six basic emotions (sadness, fear, disgust, anger, surprise, and happiness; Ekman, 1992) contain both negativity and positivity, as opposed to consisting of a single continuum between negative and positive. We examined the perceived negativity and positivity of these emotions in terms of their affective and cognitive components among Korean, Chinese, Canadian, and American students. Assessing each emotion at the cognitive and affective levels cross-culturally provides a fairly comprehensive picture of the positivity and negativity of emotions. Affective components were rated as more divergent than cognitive components. Cross-culturally, Americans and Canadians gave higher valence ratings to the salient valence of each emotion, and lower ratings to the non-salient valence of an emotion, compared to Chinese and Koreans. The results suggest that emotions encompass both positivity and negativity, and there were cross-cultural differences in reported emotions. This paradigm complements existing emotion theories, building on past research and allowing for more parsimonious explanations of cross-cultural research on emotion. PMID:28473791

  12. "Voices" and "Voces": Cultural and Linguistic Dimensions of Giftedness.

    ERIC Educational Resources Information Center

    Clark, Ellen Riojas; Gonzalez, Virginia

    1998-01-01

    Assessments of a 6-year-old Spanish monolingual and 5-year-old bilingual children included a home-language survey, parents' and teachers' ratings, cartoon conservation scales, and standardized tests. Nonverbal cognitive development was shown to influence language acquisition. Cultural and linguistic factors affected children differently. (SK)

  13. Culture and Happiness.

    PubMed

    Ye, Dezhu; Ng, Yew-Kwang; Lian, Yujun

    Culture is an important factor affecting happiness. This paper examines the predictive power of cultural factors on the cross-country differences in happiness and explores how different dimensions of cultural indices differ in their effects on happiness. Our empirical results show that the global leadership and organizational behavior effectiveness nine culture indices are all significantly related with happiness. Out of these nine indices, power distance (PDI) and gender egalitarianism (GEI) play the most important and stable role in determining subjective well-being (SWB). We further examine the relative importance of the various variables in contributing to the R-squared of the regression. The results show that PDI is the most important, accounting for 50 % of the contributions to R-squared of all variables, or equalling the combined contributions of income, population density and four other traditional variables. The contribution of GEI is 37.1 %, also well surpassing other variables. Our results remain robust even taking account of the different data for culture and SWB.

  14. Social, Cultural and Linguistic Factors Affecting the Teaching of Physical Education in the Inner City.

    ERIC Educational Resources Information Center

    Leibowitz, Harold

    The work of the inner city student differs markedly from that of the middle class teacher, resulting in communication problems between teachers and students. The major problem appears to be the clash of cultures that is sustained by the dissimilar value system of the two groups. For instance, the cultural environment of most inner city students is…

  15. Culture Shapes Empathic Responses to Physical and Social Pain

    PubMed Central

    2016-01-01

    The present research investigates the extent to which cultural background moderates empathy in response to observing someone undergoing physical or social pain. In 3 studies, we demonstrate that, East Asian and White British participants differ in both affective and cognitive components of their empathic reactions in response to someone else’s pain. Compared with East Asian participants, British participants report greater empathic concern and show lower empathic accuracy. More important, findings cannot be explained by an in-group advantage effect. Potential reasons for observed cultural differences are discussed. PMID:26950365

  16. Cultural differences in distraction processing: influence of context at retrieval.

    PubMed

    Ngo, Ka Wai Joan; Amer, Tarek; Man, Louisa; Hasher, Lynn

    2018-06-04

    Cultural differences in information processing affect perceptual judgment, attention, and memory. We investigated whether cultural differences in processing patterns, specifically East Asian participants' tendency to encode holistically, compared to Western tendencies to process analytically, affect performance on an implicit memory test. First, participants completed a 1-back task on pictures with superimposed distracting words. After a delay filled with a computerised Corsi block task, they performed a word fragment task in which some fragments could be completed with the distracting words from the 1-back task. Critically, fragments were presented with the same pictures as previously seen (matched condition), with no pictures (control condition), or with pictures from other trials on the 1-back task (mismatched condition). Non-Asian Canadian participants showed virtually no priming for distraction, independent of the reinstatement of encoding context. East Asian Canadian participants showed superior priming for fragments that had been paired with their original pictures. They did not show evidence of a detriment for the mismatched, relative to control, condition.

  17. Generality and cultural variation in the experience of regret.

    PubMed

    Breugelmans, Seger M; Zeelenberg, Marcel; Gilovich, Thomas; Huang, Wen-Hsien; Shani, Yaniv

    2014-12-01

    Regret is the prototypical decision-related emotion. Most theory and research on regret comes from the United States and Europe, but recent research has suggested potential cross-cultural differences in regret. We examined generality and cultural variation in the experience of regret. A cross-cultural study compared experiences of regret with those of disappointment and guilt as reported by participants from the United States (n = 143), the Netherlands (n = 147), Israel (n = 148), and Taiwan (n = 115). We found strong evidence for generality of the distinct emotion components of regret, compared with those of disappointment and guilt. We also found cultural variation in the frequency and intensity of regret in intrapersonal situations (regrets about outcomes affecting the self) and interpersonal regrets (regrets about outcomes affecting others). Whereas in the U.S. sample, regret was experienced more intensely in intrapersonal than interpersonal situations, both emotions were experienced more intensely in interpersonal situations in the Taiwanese sample.

  18. Cultural differences in the correction of social inferences: Does the dispositional rebound occur in an interdependent culture?

    PubMed

    Geeraert, Nicolas; Yzerbyt, Vincent Y

    2007-06-01

    Although social observers have been found to rely heavily on dispositions in their causal analysis, it has been proposed that culture strongly affects this tendency. Recent research has shown that suppressing dispositional inferences during social judgment can lead to a dispositional rebound, that is relying more on dispositional information in subsequent judgments. In the present research, we investigated whether culture also affects this rebound tendency. First, Thai and Belgian participants took part in a typical attitude attribution paradigm. Next, dispositional rebound was assessed by having participants describe a series of pictures. The dispositional rebound occurred for both Belgian and Thai participants when confronted with a forced target, but disappeared for Thai participants when the situational constraints of the target were made salient. The findings are discussed in light of the current cultural models of attribution theory.

  19. Cultural relativity in perceiving emotion from vocalizations.

    PubMed

    Gendron, Maria; Roberson, Debi; van der Vyver, Jacoba Marieta; Barrett, Lisa Feldman

    2014-04-01

    A central question in the study of human behavior is whether certain emotions, such as anger, fear, and sadness, are recognized in nonverbal cues across cultures. We predicted and found that in a concept-free experimental task, participants from an isolated cultural context (the Himba ethnic group from northwestern Namibia) did not freely label Western vocalizations with expected emotion terms. Responses indicate that Himba participants perceived more basic affective properties of valence (positivity or negativity) and to some extent arousal (high or low activation). In a second, concept-embedded task, we manipulated whether the target and foil on a given trial matched in both valence and arousal, neither valence nor arousal, valence only, or arousal only. Himba participants achieved above-chance accuracy only when foils differed from targets in valence only. Our results indicate that the voice can reliably convey affective meaning across cultures, but that perceptions of emotion from the voice are culturally variable.

  20. Cross-cultural differences in somatic awareness and interoceptive accuracy: a review of the literature and directions for future research

    PubMed Central

    Ma-Kellams, Christine

    2014-01-01

    This review examines cross-cultural differences in interoception and the role of culturally bound epistemologies, historical traditions, and contemplative practices to assess four aspects of culture and interoception: (1) the extent to which members from Western and non-Western cultural groups exhibit differential levels of interoceptive accuracy and somatic awareness; (2) the mechanistic origins that can explain these cultural differences, (3) culturally bound behavioral practices that have been empirically shown to affect interoception, and (4) consequences for culturally bound psychopathologies. The following outlines the scope of the scientific review. Part 1 reviews studies on cultural variation in spontaneous somatic word use, linguistic expressions, traditional medical practices, and empirical laboratory studies to assess the evidence for cultural differences in somatic processes. Integration of these findings suggests a startling paradox: on the one hand, non-Western cultures consistently exhibit heightened somatic focus and awareness across a variety of contexts; on the other hand, non-Western cultures also exhibit less interoceptive accuracy in laboratory studies. Part 2 discusses the various mechanistic explanations that have been proposed to explain these cultural differences in somatic awareness and interoceptive accuracy, focusing on cultural schemas and epistemologies. Part 3 addresses the behavioral and contemplative practices that have been proposed as possible “interventions,” or methods of cultivating bodily awareness and perceptual accuracy. Finally, Part 4 reviews the consequences of interoception for psychopathology, including somatization, body dysmorphia, eating disorders, and anxiety disorders. PMID:25520688

  1. Cross-cultural differences in somatic awareness and interoceptive accuracy: a review of the literature and directions for future research.

    PubMed

    Ma-Kellams, Christine

    2014-01-01

    This review examines cross-cultural differences in interoception and the role of culturally bound epistemologies, historical traditions, and contemplative practices to assess four aspects of culture and interoception: (1) the extent to which members from Western and non-Western cultural groups exhibit differential levels of interoceptive accuracy and somatic awareness; (2) the mechanistic origins that can explain these cultural differences, (3) culturally bound behavioral practices that have been empirically shown to affect interoception, and (4) consequences for culturally bound psychopathologies. The following outlines the scope of the scientific review. Part 1 reviews studies on cultural variation in spontaneous somatic word use, linguistic expressions, traditional medical practices, and empirical laboratory studies to assess the evidence for cultural differences in somatic processes. Integration of these findings suggests a startling paradox: on the one hand, non-Western cultures consistently exhibit heightened somatic focus and awareness across a variety of contexts; on the other hand, non-Western cultures also exhibit less interoceptive accuracy in laboratory studies. Part 2 discusses the various mechanistic explanations that have been proposed to explain these cultural differences in somatic awareness and interoceptive accuracy, focusing on cultural schemas and epistemologies. Part 3 addresses the behavioral and contemplative practices that have been proposed as possible "interventions," or methods of cultivating bodily awareness and perceptual accuracy. Finally, Part 4 reviews the consequences of interoception for psychopathology, including somatization, body dysmorphia, eating disorders, and anxiety disorders.

  2. Cultural Variation in Implicit Mental Illness Stigma.

    PubMed

    Cheon, Bobby K; Chiao, Joan Y

    2012-10-01

    Culture shapes how individuals perceive and respond to others with mental illness. Prior studies have suggested that Asians and Asian Americans typically endorse greater stigma of mental illness compared to Westerners (White Europeans and Americans). However, whether these differences in stigma arise from cultural variations in automatic affective reactions or deliberative concerns of the appropriateness of one's reactions to mental illness remains unknown. Here we compared implicit and explicit attitudes toward mental illness among Asian and Caucasian Americans. Asian Americans showed stronger negative implicit attitudes toward mental illness relative to Caucasian Americans, suggesting that cultural variation in stigma of mental illness can be observed even when concerns regarding the validity and appropriateness of one's attitudes toward mental illness are minimized. Asian Americans also explicitly endorsed greater desire for social distance from mental illness relative to Caucasian Americans. These findings suggest that cultural variations in mental illness stigma may arise from cultural differences in automatic reactions to mental illness, though cultural variations in deliberative processing may further shape differences in these immediate reactions to mental illness.

  3. Type of culture media does not affect embryo kinetics: a time-lapse analysis of sibling oocytes.

    PubMed

    Basile, Natalia; Morbeck, Dean; García-Velasco, Juan; Bronet, Fernando; Meseguer, Marcos

    2013-03-01

    Are the morphokinetics of growing embryos affected by the type of culture media utilized? Morphokinetic parameters used for embryo selection are not affected between the two different concept culture media analyzed. Studies on the effect of culture media on human embryos have focused on evaluating different in-house and commercially available media as well as comparing outcomes among different commercial media. Nonetheless, the evaluation of embryo development in these studies was based on static observations and very little is known from a dynamic point of view. Prospective cohort study, October 2010 and April 2011. University-affiliated infertility center. Patients undergoing egg donation (n = 75) in which embryos were cultured with two different types of media in a time-lapse system. Embryo development was analyzed with time-lapse imaging for single step media (Global®) and sequential media (Sage® Cleavage). Variables studied included the timing to two cells (t2), three cells (t3), four cells (t4) and five cells (t5) as well as the length of the second cell cycle (cc2 = t3 - t2) and the synchrony in the division from two to four cells (s2 = t4 - t3). Implantation and clinical pregnancy rates were also analyzed. No statistically significant differences were observed between the two media for all the variables analyzed. When analyzing the percentage of embryos falling within the optimal ranges proposed for s2, cc2 and t5, we did not find significant differences between the two media. Pregnancy and implantation rates were similar for the three types of transfers: 48.0% (CI 95% 28.4-67.6) and 42.0% (CI 95% 22.5-61.4) with Global media; 58.8% (CI 95% 35.4-82.2) and 38.2% (CI 95% 15.0-61.4) with Cleavage media; and 58.1% (CI 95% 40.7-75.4) and 37.1% (CI 95% 22.1-52.1) with mixed transferred, respectively. Multiple implantations (twins) were also similar among the three groups, with 24.0% (CI 95% 9.3-45.1) for transfers with embryos cultured in Global media, 17.6% (CI 95% 3.7-43.3) for transfers with embryos cultured in Cleavage media and 22.5% (CI 95% 9.5-41.0) with mixed transfers. The study was not powered to test differences in pregnancy rates between the two culture media, as this was not the hypothesis tested. Results are based on observations with embryos from oocyte donors and need to be repeated with embryos from infertile patients of different ages. The absence of differences in morphokinetics between two different media concepts validates the algorithm for embryo selection in diverse culture conditions. No specific funding was obtained for this study; it was solely funded by IVI. None of the authors have any economic affiliation with Unisense Fertilitech A/S but IVI is a minor shareholder in Unisense Fertilitech A/S.

  4. Addressing Cultural Diversity: Effects of a Problem-Based Intercultural Learning Unit

    ERIC Educational Resources Information Center

    Busse, Vera; Krause, Ulrike-Marie

    2015-01-01

    This article explores to what extent a problem-based learning unit in combination with cooperative learning and affectively oriented teaching methods facilitates intercultural learning. As part of the study, students reflected on critical incidents, which display misunderstandings or conflicts that arise as a result of cultural differences. In…

  5. Understanding Chinese Immigrant and European American Mothers' Expressions of Warmth

    ERIC Educational Resources Information Center

    Cheah, Charissa S. L.; Li, Jin; Zhou, Nan; Yamamoto, Yoko; Leung, Christy Y. Y.

    2015-01-01

    Maternal warmth, the quality of the affectional bond between mothers and their children, has been found to be consistently associated with children's positive developmental outcomes in Western cultures. However, researchers debate the potential differences in the cultural meanings of maternal warmth, particularly between Chinese and European…

  6. Cultural Complexity That Affects Young Children's Contemporary Growth, Change, and Learning.

    ERIC Educational Resources Information Center

    Hyun, Eunsook

    Based on the view that the group orientation to multicultural education reinforces group stereotyping and seldom allows acknowledgement of diverse children's unique capabilities and differences or helps children build self-identity while learning to appreciate others, this paper presents and discusses contemporary cultures of young children's…

  7. The Social Meaning of Leisure in Uganda and America.

    ERIC Educational Resources Information Center

    Crandall, Rich; Thompson, Richard W.

    1978-01-01

    This paper analyzes cross-culturally the importance of social contact for leisure. The general findings of considerable similarity in evaluating preferences and the importance of social considerations provide a basis for preliminary comparisons and suggest that similar factors can affect leisure preferences in different cultural settings.…

  8. The Institution of Advertising: Predictors of Cross-National Differences in Consumer Confidence.

    ERIC Educational Resources Information Center

    Zinkhan, George M.; Balazs, Anne L.

    1998-01-01

    Contributes to scholarship on advertising and cross-cultural studies by exploring cultural factors affecting customer confidence in advertising. Uses a sample of 16 European nations to test G. Hofstede's theory of cross-national values. Finds that Hofstede's dimensions of uncertainty avoidance, masculinity, and individualism are important…

  9. Gender, Religion, and Sociopolitical Issues in Cross-Cultural Online Education

    ERIC Educational Resources Information Center

    Zaidi, Zareen; Verstegen, Daniëlle; Naqvi, Rahat; Morahan, Page; Dornan, Tim

    2016-01-01

    Cross-cultural education is thought to develop critical consciousness of how unequal distributions of power and privilege affect people's health. Learners in different sociopolitical settings can join together in developing critical consciousness--awareness of power and privilege dynamics in society--by means of communication technology. The aim…

  10. Access to cancer services--do culture and ethnicity make a difference?

    PubMed

    Thompson, Rose; Van Der Molen, Beverley

    2009-01-01

    This module discusses the diverse cultural needs of people affected by cancer and how those needs can be assessed and met. The UK experience with Black or Minority Ethnic (BME) communities will be used to explore the issues of awareness of and detection of cancer.

  11. Comparing the Use of Evidence and Culture in Targeted Colorectal Cancer Communication for African Americans

    PubMed Central

    Thompson, Vetta L. Sanders; Kalesan, Bindu; Wells, Anjanette; Williams, Sha-Lai; Caito, Nicole M.

    2010-01-01

    Objective This study examined the effects (affective reactions, cognitive reactions and processing, perceived benefits and barriers and intent to screen) of targeted Peripheral + Evidential (PE) and Peripheral+Evidential+Socio-cultural (PE+SC) colorectal cancer communications. Methods This study was a two-arm randomized control study of cancer communication effects on affective, cognitive processing, and behavioral outcomes over a 22-week intervention. There were 771 African American participants, 45 to 75 years, participating in the baseline survey related to CRC screening. Three follow-up interviews that assessed intervention effects on affective response to the publications, cognitive processing, and intent to obtain CRC screening were completed. Results There were no statistically significant differences between PE and PE+SC intervention groups for affect, cognitive processing or intent to screen. However, there were significant interactions effects on outcome variables. Conclusions The advantages and disadvantages of PE+SC targeted cancer communications and implications of sex differences are considered. Practice Implications While there do not appear to be significant differences in behavioral outcomes when using PE and PE+SC strategies, there appear to be subtle differences in affective and cognitive processing outcomes related to medical suspicion and ethnic identity, particularly as it relates to gender. PMID:20702056

  12. Comparing the use of evidence and culture in targeted colorectal cancer communication for African Americans.

    PubMed

    Thompson, Vetta L Sanders; Kalesan, Bindu; Wells, Anjanette; Williams, Sha-Lai; Caito, Nicole M

    2010-12-01

    This study examined the effects (affective reactions, cognitive reactions and processing, perceived benefits and barriers and intent to screen) of targeted peripheral+evidential (PE) and peripheral+evidential+socio-cultural (PE+SC) colorectal cancer communications. This study was a two-arm randomized control study of cancer communication effects on affective, cognitive processing, and behavioral outcomes over a 22-week intervention. There were 771 African American participants, 45-75 years, participating in the baseline survey related to CRC screening. Three follow-up interviews that assessed intervention effects on affective response to the publications, cognitive processing, and intent to obtain CRC screening were completed. There were no statistically significant differences between PE and PE+SC intervention groups for affect, cognitive processing or intent to screen. However, there were significant interactions effects on outcome variables. The advantages and disadvantages of PE+SC targeted cancer communications and implications of sex differences are considered. While there do not appear to be significant differences in behavioral outcomes when using PE and PE+SC strategies, there appear to be subtle differences in affective and cognitive processing outcomes related to medical suspicion and ethnic identity, particularly as it relates to gender. Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.

  13. Initial pH of medium affects organic acids production but do not affect phosphate solubilization.

    PubMed

    Marra, Leandro M; de Oliveira-Longatti, Silvia M; Soares, Cláudio R F S; de Lima, José M; Olivares, Fabio L; Moreira, Fatima M S

    2015-06-01

    The pH of the culture medium directly influences the growth of microorganisms and the chemical processes that they perform. The aim of this study was to assess the influence of the initial pH of the culture medium on the production of 11 low-molecular-weight organic acids and on the solubilization of calcium phosphate by bacteria in growth medium (NBRIP). The following strains isolated from cowpea nodules were studied: UFLA03-08 (Rhizobium tropici), UFLA03-09 (Acinetobacter sp.), UFLA03-10 (Paenibacillus kribbensis), UFLA03-106 (Paenibacillus kribbensis) and UFLA03-116 (Paenibacillus sp.). The strains UFLA03-08, UFLA03-09, UFLA03-10 and UFLA03-106 solubilized Ca3(PO4)2 in liquid medium regardless of the initial pH, although without a significant difference between the treatments. The production of organic acids by these strains was assessed for all of the initial pH values investigated, and differences between the treatments were observed. Strains UFLA03-09 and UFLA03-10 produced the same acids at different initial pH values in the culture medium. There was no correlation between phosphorus solubilized from Ca3(PO4)2 in NBRIP liquid medium and the concentration of total organic acids at the different initial pH values. Therefore, the initial pH of the culture medium influences the production of organic acids by the strains UFLA03-08, UFLA03-09, UFLA03-10 and UFLA03-106 but it does not affect calcium phosphate solubilization.

  14. Descent with modification and the archaeological record

    PubMed Central

    Shennan, Stephen

    2011-01-01

    Recent years have seen major advances in our understanding of the way in which cultural transmission takes place and the factors that affect it. The theoretical foundations of those advances have been built by postulating the existence of a variety of different processes and deriving their consequences mathematically or by simulation. The operation of these processes in the real world can be studied through experiment and naturalistic observation. In contrast, archaeologists have an ‘inverse problem’. For them the object of study is the residues of different behaviours represented by the archaeological record and the problem is to infer the microscale processes that produced them, a vital task for cultural evolution since this is the only direct record of past cultural patterns. The situation is analogous to that faced by population geneticists scanning large number of genes and looking for evidence of selection as opposed to drift, but more complicated for many reasons, not least the enormous variety of different forces that affect cultural transmission. This paper reviews the progress that has been made in inferring processes from patterns and the role of demography in those processes, together with the problems that have arisen. PMID:21357229

  15. Cultural perceptions and clinical experiences of nursing students in Eastern Turkey.

    PubMed

    Karatay, G; Bowers, B; Karadağ, E B; Demir, M C

    2016-12-01

    This study explored Turkish nursing students' perceptions of providing care to patients culturally different from themselves. Increasing migration will increase the need for nurses to provide care across cultural groups. Twenty one students in the second year of a 4-year nursing programme participated in 3 focus groups. Data were analysed using directed content analysis. Research questions were based on Campinha-Bacote's model. Three themes were identified: perceived cultural barriers, perceived cultural facilitators and identifying culturally sensitive actions. Generally, students were able to define culture but were unable to say how culture would affect nursing practice. Students were unprepared to practice in a multicultural setting. Cultural awareness is insufficient for determining how to respond to cultural differences. The study is limited by its restriction to a single school of nursing and a single curriculum. The multiple, ongoing political, religious and ethnic conflicts will require nurses to provide care to patients from other cultural groups, in some instances to people identified as adversaries to the group the nurse may represent. Understanding cultural differences is insufficient to do this effectively. Learning culturally competent care requires opportunities to provide, be guided through and reflect on care to individuals from different cultural groups. Standards for culturally competent care should be adopted by all care delivery settings. Public and organizational policies openly declaring healthcare settings as cultural safe zones, and explicit organizational commitment to culturally safe care, would set clear expectations for providers and help ensure a positive patient experience. © 2016 International Council of Nurses.

  16. Cultural differences in emotion: differences in emotional arousal level between the East and the West.

    PubMed

    Lim, Nangyeon

    2016-06-01

    Whether emotion is universal or social is a recurrent issue in the history of emotion study among psychologists. Some researchers view emotion as a universal construct, and that a large part of emotional experience is biologically based. However, emotion is not only biologically determined, but is also influenced by the environment. Therefore, cultural differences exist in some aspects of emotions, one such important aspect of emotion being emotional arousal level. All affective states are systematically represented as two bipolar dimensions, valence and arousal. Arousal level of actual and ideal emotions has consistently been found to have cross-cultural differences. In Western or individualist culture, high arousal emotions are valued and promoted more than low arousal emotions. Moreover, Westerners experience high arousal emotions more than low arousal emotions. By contrast, in Eastern or collectivist culture, low arousal emotions are valued more than high arousal emotions. Moreover, people in the East actually experience and prefer to experience low arousal emotions more than high arousal emotions. Mechanism of these cross-cultural differences and implications are also discussed.

  17. Does cultural and linguistic diversity affect health-related outcomes for people with stroke at discharge from hospital?

    PubMed

    Davies, Sarah E; Dodd, Karen J; Hill, Keith D

    2017-04-01

    Primary purpose to determine if cultural and linguistic diversity affects health-related outcomes in people with stroke at discharge from hospital and secondary purpose to explore whether interpreter use alters these outcomes. Systematic search of: Cochrane, PEDro, CINAHL, Medline, Pubmed, Embase, PsycINFO and Ageline databases. Publications were classified into whether they examined the impact of diversity in culture, or language or culture and language combined. Quality of evidence available was summarized using Best Evidence Synthesis. Eleven studies met inclusion criteria and were reviewed. Best Evidence Synthesis indicated conflicting evidence about the impact of culture alone and language barriers alone on health-related outcomes. There was strong evidence that hospital length of stay does not differ between groups when the combined impact of culture and language was investigated. Conflicting evidence was found for other outcomes including admission, discharge and change in FIM scores, and post-hospital discharge living arrangements. It is unknown if interpreter use alters health-related outcomes, because this was infrequently reported. The current limited research suggests that cultural and linguistic diversity does not appear to impact on health-related outcomes at discharge from hospital for people who have had a stroke, however further research is needed to address identified gaps. Implications for Rehabilitation The different language, culture and beliefs about health demonstrated by patients with stroke from minority groups in North America do not appear to significantly impact on their health-related outcomes during their admission to hospital. It is not known whether interpreter use influences outcomes in stroke rehabilitation because there is insufficient high quality research in this area. Clinicians in countries with different health systems and different cultural and linguistic groups within their communities need to view the results with caution as further investigation is required outside North America to ensure optimal and equitable care for these groups. In the absence of clear outcomes from high quality research, clinicians should ensure patients and their families have an optimal understanding of the health condition, the rehabilitation process and the service system, irrespective of language or cultural differences.

  18. The Relation Between Valence and Arousal in Subjective Experience Varies With Personality and Culture.

    PubMed

    Kuppens, Peter; Tuerlinckx, Francis; Yik, Michelle; Koval, Peter; Coosemans, Joachim; Zeng, Kevin J; Russell, James A

    2017-08-01

    While in general arousal increases with positive or negative valence (a so-called V-shaped relation), there are large differences among individuals in how these two fundamental dimensions of affect are related in people's experience. In two studies, we examined two possible sources of this variation: personality and culture. In Study 1, participants (Belgian university students) recalled a recent event that was characterized by high or low valence or arousal and reported on their feelings and their personality in terms of the Five-Factor Model. In Study 2, participants from Canada, China/Hong Kong, Japan, Korea, and Spain reported on their feelings in a thin slice of time and on their personality. In Study 1, we replicated the V-shape as characterizing the relation between valence and arousal, and identified personality correlates of experiencing particular valence-arousal combinations. In Study 2, we documented how the V-shaped relation varied as a function of Western versus Eastern cultural background and personality. The results showed that the steepness of the V-shaped relation between valence and arousal increases with Extraversion within cultures, and with a West-East distinction between cultures. Implications for the personality-emotion link and research on cultural differences in affect are discussed. © 2016 Wiley Periodicals, Inc.

  19. The partial pressure of oxygen affects biomarkers of oxidative stress in cultured rainbow trout (Oncorhynchus mykiss) hepatocytes.

    PubMed

    Finne, E F; Olsvik, P A; Berntssen, M H G; Hylland, K; Tollefsen, K E

    2008-09-01

    Oxidative stress, the imbalance between production of reactive oxygen species and the cellular detoxification of these reactive compounds, is believed to be involved in the pathology of various diseases. Several biomarkers for oxidative stress have been proposed to serve as tools in toxicological and ecotoxicological research. Not only may exposure to various pro-oxidants create conditions of cellular oxidative stress, but hyperoxic conditions may also increase the production of reactive oxygen species. The objective of the current study was to determine the extent to which differences in oxygen partial pressure would affect biomarkers of oxidative stress in a primary culture of hepatocytes from rainbow trout (Oncorhynchus mykiss). Membrane integrity, metabolic activity, levels of total and oxidized glutathione (tGSH/GSSG) was determined, as well as mRNA expression levels of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GSSG-R), gamma-glutamyl-cystein synthetase (GCS) and thioredoxin (TRX). The results show that different biomarkers of oxidative stress are affected when the cell culture is exposed to atmospheric oxygen, and that changes such as increased GSSG content and induction of GSSG-R and GSH-Px can be reduced by culturing the cells under lower oxygen tension. Oxygen tension may thus influence results of in vitro based cell research and is particularly important when assessing parameters in the antioxidant defence system. Further research is needed to establish the magnitude of this effect in different cellular systems.

  20. Age differences in cognitive performance: A study of cultural differences in Historical Context.

    PubMed

    Ojeda, Natalia; Aretouli, Eleni; Peña, Javier; Schretlen, David J

    2016-03-01

    Ethnicity and cultural experience can affect neuropsychological performance, but they are rarely assessed in historical context. Attention measures are considered strongly biologically determined and therefore potentially culture-fair. In this study, we assessed the cross-cultural equivalence of Spanish and English versions of the Trail Making Test (TMT; Reitan, 1958, Perceptual and Motor Skills, 8, 271-276) and the Brief Test of Attention (BTA; Schretlen et al., 1996, The Clinical Neuropsychologist, 10, 80-89) in two large samples of Americans (N = 203) and Spaniards (N = 213), divided into younger and older subgroups. The older Spaniards lived under Franco's political regime (1936-1975), whereas the Americans never experienced such repression. Overall, TMT performance was culture-sensitive, whereas BTA performance was not. However, when both groups were stratified by age, cultural differences in TMT performance were restricted to older participants, suggesting that historical experience across generations might have contributed to the observed differences in cognitive performance. Even such basic cognitive processes as attention, working memory, and resource sharing might be shaped to some degree by historical experiences that contribute to cultural differences. © 2015 The Authors. Journal of Neuropsychology published by John Wiley & Sons Ltd on behalf of British Psychological Society.

  1. Differential bacterial load on components of total knee prosthesis in patients with prosthetic joint infection.

    PubMed

    Holinka, Johannes; Pilz, Magdalena; Hirschl, Alexander M; Graninger, Wolfgang; Windhager, Reinhard; Presterl, Elisabeth

    2012-10-01

    The purpose of our study was to evaluate and quantify the bacterial adherence on different components of total knee prosthesis with the sonication culture method. Explanted components of all patients with presumptive prosthetic or implant infection were treated by sonication separately in sterile containers to dislodge the adherent bacteria from the surfaces and cultured. The bacterial load of the different knee components (femur, tibia, PE-inlay and patella) was evaluated by counting of colony-forming units (CFU) dislodged from the components surfaces using the sonication culture method. Overall, 27 patients had positive sonication cultures of explanted total knee prostheses. Microorganisms were detected from 88 of 100 explanted components. Twenty femoral components were culture positive and 7 negative, 23 tibial components as well as 23 polyethylene (PE) platforms had positive microorganism detection from the surface. Staphylococcus epidermidis adhered to the highest number of components whereas Staphylococcus aureus yielded the highest load of CFU in the sonication cultures. Although not significant, PE-inlays and tibial components were most often affected. The highest CFU count was detected in polyethylene components. The sonication culture method is a reliable method to detect bacteria from the components. Additionally, the results demonstrate that bacterial adherence is not affecting a single component of knee prosthesis only. Thus, in septic revision surgery partial prosthetic exchange or exchange of single polyethylene components alone may be not sufficient.

  2. Esthetic preferences of laypersons of different cultures and races with regard to smile attractiveness.

    PubMed

    Sadrhaghighi, Amir Houman; Zarghami, Afsaneh; Sadrhaghighi, Shahrzad; Mohammadi, Amir; Eskandarinezhad, Mahsa

    2017-01-01

    Culture and ethnicity are among the factors affecting esthetic judgment of individuals. This study aimed to assess the acceptability threshold of variations in four components of an esthetic smile namely vertical lip thickness, dental midline deviation, buccal corridor, and the golden ratio in maxillary lateral incisors display among laypersons of different races and cultures. Raters (n = 35 in each city) among laypersons of nine cities namely Istanbul, Isfahan, Tabriz, Tehran, Doha, Rome, Sydney, Chicago, and Yazd, were given a photo album containing 27 random images of an attractive female smile, digitally altered with regard to the four smile components. They scored each picture from 0 to 100 in terms of smile attractiveness. Data were analyzed using SPSS 13 and the acceptability threshold for each component was calculated in each city using the Spearman and Wilcoxon tests. P< 0.05 was considered statistically significant. No significant differences were noted with regard to the increased vertical lip thickness, and an acceptability threshold could not be determined for it. The acceptability thresholds for midline deviations, buccal corridor, and the golden ratio were different among different cities. One-millimeter increase in the displayed width of maxillary lateral incisors was more desirable than the golden ratio standard width. Culture and race may significantly affect the esthetic preference of individuals with regard to smile attractiveness.

  3. Individual Differences in Verbal and Non-Verbal Affective Responses to Smells: Influence of Odor Label Across Cultures.

    PubMed

    Ferdenzi, Camille; Joussain, Pauline; Digard, Bérengère; Luneau, Lucie; Djordjevic, Jelena; Bensafi, Moustafa

    2017-01-01

    Olfactory perception is highly variable from one person to another, as a function of individual and contextual factors. Here, we investigated the influence of 2 important factors of variation: culture and semantic information. More specifically, we tested whether cultural-specific knowledge and presence versus absence of odor names modulate odor perception, by measuring these effects in 2 populations differing in cultural background but not in language. Participants from France and Quebec, Canada, smelled 4 culture-specific and 2 non-specific odorants in 2 conditions: first without label, then with label. Their ratings of pleasantness, familiarity, edibility, and intensity were collected as well as their psychophysiological and olfactomotor responses. The results revealed significant effects of culture and semantic information, both at the verbal and non-verbal level. They also provided evidence that availability of semantic information reduced cultural differences. Semantic information had a unifying action on olfactory perception that overrode the influence of cultural background. © The Author 2016. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  4. Bacillus Classification Based on Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry-Effects of Culture Conditions.

    PubMed

    Shu, Lin-Jie; Yang, Yu-Liang

    2017-11-14

    Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a reliable and rapid technique applied widely in the identification and classification of microbes. MALDI-TOF MS has been used to identify many endospore-forming Bacillus species; however, endospores affect the identification accuracy when using MALDI-TOF MS because they change the protein composition of samples. Since culture conditions directly influence endospore formation and Bacillus growth, in this study we clarified how culture conditions influence the classification of Bacillus species by using MALDI-TOF MS. We analyzed members of the Bacillus subtilis group and Bacillus cereus group using different incubation periods, temperatures and media. Incubation period was found to affect mass spectra due to endospores which were observed mixing with vegetative cells after 24 hours. Culture temperature also resulted in different mass spectra profiles depending on the temperature best suited growth and sporulation. Conversely, the four common media for Bacillus incubation, Luria-Bertani agar, nutrient agar, plate count agar and brain-heart infusion agar did not result in any significant differences in mass spectra profiles. Profiles in the range m/z 1000-3000 were found to provide additional data to the standard ribosomal peptide/protein region m/z 3000-15000 profiles to enable easier differentiation of some highly similar species and the identification of new strains under fresh culture conditions. In summary, control of culture conditions is vital for Bacillus identification and classification by MALDI-TOF MS.

  5. Dimensions of patient safety culture in family practice.

    PubMed

    Palacios-Derflingher, Luz; O'Beirne, Maeve; Sterling, Pam; Zwicker, Karen; Harding, Brianne K; Casebeer, Ann

    2010-01-01

    Safety culture has been shown to affect patient safety in healthcare. While the United States and United Kingdom have studied the dimensions that reflect patient safety culture in family practice settings, to date, this has not been done in Canada. Differences in the healthcare systems between these countries and Canada may affect the dimensions found to be relevant here. Thus, it is important to identify and compare the dimensions from the United States and the United Kingdom in a Canadian context. The objectives of this study were to explore the dimensions of patient safety culture that relate to family practice in Canada and to determine if differences and similarities exist between dimensions found in Canada and those found in previous studies undertaken in the United States and the United Kingdom. A qualitative study was undertaken applying thematic analysis using focus groups with family practice offices and supplementary key stakeholders. Analysis of the data indicated that most of the dimensions from the United States and United Kingdom are appropriate in our Canadian context. Exceptions included owner/managing partner/leadership support for patient safety, job satisfaction and overall perceptions of patient safety and quality. Two unique dimensions were identified in the Canadian context: disclosure and accepting responsibility for errors. Based on this early work, it is important to consider differences in care settings when understanding dimensions of patient safety culture. We suggest that additional research in family practice settings is critical to further understand the influence of context on patient safety culture.

  6. Big Five personality traits: are they really important for the subjective well-being of Indians?

    PubMed

    Tanksale, Deepa

    2015-02-01

    This study empirically examined the relationship between the Big Five personality traits and subjective well-being (SWB) in India. SWB variables used were life satisfaction, positive affect and negative affect. A total of 183 participants in the age range 30-40 years from Pune, India, completed the personality and SWB measures. Backward stepwise regression analysis showed that the Big Five traits accounted for 17% of the variance in life satisfaction, 35% variance in positive affect and 28% variance in negative affect. Conscientiousness emerged as the strongest predictor of life satisfaction. In line with the earlier research findings, neuroticism and extraversion were found to predict negative affect and positive affect, respectively. Neither openness to experience nor agreeableness contributed to SWB. The research emphasises the need to revisit the association between personality and SWB across different cultures, especially non-western cultures. © 2014 International Union of Psychological Science.

  7. The relation between language, culture, and thought.

    PubMed

    Imai, Mutsumi; Kanero, Junko; Masuda, Takahiko

    2016-04-01

    The relationship between culture, language, and thought has long been one of the most important topics for those who wish to understand the nature of human cognition. This issue has been investigated for decades across a broad range of research disciplines. However, there has been scant communication across these different disciplines, a situation largely arising through differences in research interests and discrepancies in the definitions of key terms such as 'culture,' 'language,' and 'thought.' This article reviews recent trends in research on the relation between language, culture and thought to capture how cognitive psychology and cultural psychology have defined 'language' and 'culture,' and how this issue was addressed within each research discipline. We then review recent research conducted in interdisciplinary perspectives, which directly compared the roles of culture and language. Finally, we highlight the importance of considering the complex interplay between culture and language to provide a comprehensive picture of how language and culture affect thought. Copyright © 2015 Elsevier Ltd. All rights reserved.

  8. Growth Phase, Oxygen, Temperature, and Starvation Affect the Development of Viable but Non-culturable State of Vibrio cholerae.

    PubMed

    Wu, Bin; Liang, Weili; Kan, Biao

    2016-01-01

    Vibrio cholerae can enter into a viable but non-culturable (VBNC) state in order to survive in unfavorable environments. In this study, we studied the roles of five physicochemical and microbiological factors or states, namely, different strains, growth phases, oxygen, temperature, and starvation, on the development of VBNC of V. cholerae in artificial sea water (ASW). Different strains of the organism, the growth phase, and oxygen levels affected the progress of VBNC development. It was found that the VBNC state was induced faster in V. cholerae serogroup O1 classical biotype strain O395 than in O1 El Tor biotype strains C6706 and N16961. When cells in different growth phases were used for VBNC induction, stationary-phase cells lost their culturability more quickly than exponential-phase cells, while induction of a totally non-culturable state took longer to achieve for stationary-phase cells in all three strains, suggesting that heterogeneity of cells should be considered. Aeration strongly accelerated the loss of culturability. During the development of the VBNC state, the culturable cell count under aeration conditions was almost 10(6)-fold lower than under oxygen-limited conditions for all three strains. The other two factors, temperature and nutrients-rich environment, may prevent the induction of VBNC cells. At 22 or 37°C in ASW, most of the cells rapidly died and the culturable cell count reduced from about 10(8) to 10(6)-10(5) CFU/mL. The total cell counts showed that cells that lost viability were decomposed, and the viable cell counts were the same as culturable cell counts, indicating that the cells did not reach the VBNC state. VBNC state development was blocked when ASW was supplied with Luria-Bertani broth (LB), but it was not affected in ASW with M9, suggesting that specific nutrients in LB may prevent the development of VBNC state. These results revealed that the five factors evaluated in this study had different roles during the progress of VBNC induction. Changing a single factor could influence and even block the development of the VBNC state. These findings provide new insight to help design further studies to better understand the mechanisms which trigger the development and regulation of the VBNC state.

  9. Maternofetal outcome of asymptomatic bacteriuria among pregnant women in a Nigerian Teaching Hospital.

    PubMed

    Izuchukwu, Kenneth Ebele; Oranu, Emmanuel Okwudili; Bassey, Goddy; Orazulike, Ngozi Clare

    2017-01-01

    Asymptomatic bacteriuria has been reported to be associated with adverse pregnancy outcome. This study sought to determine the prevalence and complications of asymptomatic bacteriuria amongst parturient in the University of Port Harcourt Teaching Hospital (UPTH). The study was a prospective cohort study involving 220 eligible antenatal attendees. Urine culture and sensitivity was conducted for each participant and the fetomaternal outcome between affected and unaffected women were compared and p value <0.05 was considered significant. Sixty-five of the participants had asymptomatic bacteriuria giving a prevalence of 29.5%. Twenty-three (35.4%) cultures yielded Klebsiella spp while Fifty-eight (89%) of the cultured organisms were sensitive to Nitrofurantoin. There was no statistical difference in the rate of prelabour rupture of membranes, preeclampsia, preterm delivery, birth asphyxia and low birth weight between affected and unaffected women. Contrary to widely held view, there was no significant increase in adverse pregnancy outcome amongst affected women.

  10. Some implications of populus intensive culture on nongame birds

    Treesearch

    Richard L. Verch

    1983-01-01

    Intensive culture of Populus will affect nongame bird habitat. Conversion of old fields to Populus plantations will destroy habitat favorable to certain species and produce habitat that will attract different species. Effects of this conversion can be lessened by planting plantations with irregular shapes and by leaving patches (.4 hectares) of...

  11. Nature and Nurture: Williams Syndrome across Cultures

    ERIC Educational Resources Information Center

    Zitzer-Comfort, Carol; Doyle, Teresa; Masataka, Nobuo; Korenberg, Julie; Bellugi, Ursula

    2007-01-01

    This study is concerned with ways in which children with Williams syndrome (WS), a rare neurodevelopmental disorder arising from a hemizygous deletion in chromosome band 7q11.23 including the gene for elastin (ELN) and approximately 20 surrounding genes, are affected by social mores of vastly differing cultures: the United States and Japan. WS…

  12. Italian and Swedish adolescents: differences and associations in subjective well-being and psychological well-being

    PubMed Central

    Sagone, Elisabetta; De Caroli, Maria Elvira; Nima, Ali Al

    2017-01-01

    Background One important aspect of subjective judgments about one’s well-being (i.e., subjective well-being: life satisfaction, positive affect, and negative affect) is that cultural features, such as, nationality seem to shape cognitive judgments about the “the ideal life.” In this comparative study we examined differences in subjective well-being and psychological well-being between Italian and Swedish adolescents and tested if the relationship between the three constructs of subjective well-being (i.e., satisfaction with life, positive affect, and negative affect) and psychological well-being was moderated by the adolescents’ nationality. Method Italian (n = 255) and Swedish (n = 277) adolescents answered to the Satisfaction with Life Scale, the Positive Affect Negative Affect Schedule, and Ryff’s Scales of Psychological Well-Being. Differences between samples were tested using a Multiple Analysis of Variance. We also conducted a multiple group analysis (Italy and Sweden) using Structural Equation Modelling to investigate the relationship between all three subjective well-being constructs and psychological well-being. Results Italian adolescents scored significantly higher in satisfaction with life than Swedish adolescents. Additionally, across countries, girls scored significantly higher in negative affect than boys. In both countries, all three constructs of subjective well-being were significantly associated to adolescents’ psychological well-being. Nevertheless, while the effect of the relationship between affect and psychological well-being was almost the same across countries, life satisfaction was more strongly related to psychological well-being among Swedish adolescents. Conclusions The present study shows that there are larger variations between these two cultures in the cognitive construct of subjective well-being than in the affective construct. Accordingly, associations between the cognitive component, not the affective component, of subjective well-being and psychological well-being differ between countries as well. PMID:28097069

  13. Italian and Swedish adolescents: differences and associations in subjective well-being and psychological well-being.

    PubMed

    Garcia, Danilo; Sagone, Elisabetta; De Caroli, Maria Elvira; Nima, Ali Al

    2017-01-01

    One important aspect of subjective judgments about one's well-being (i.e., subjective well-being: life satisfaction, positive affect, and negative affect) is that cultural features, such as, nationality seem to shape cognitive judgments about the "the ideal life." In this comparative study we examined differences in subjective well-being and psychological well-being between Italian and Swedish adolescents and tested if the relationship between the three constructs of subjective well-being (i.e., satisfaction with life, positive affect, and negative affect) and psychological well-being was moderated by the adolescents' nationality. Italian ( n = 255) and Swedish ( n = 277) adolescents answered to the Satisfaction with Life Scale, the Positive Affect Negative Affect Schedule, and Ryff's Scales of Psychological Well-Being. Differences between samples were tested using a Multiple Analysis of Variance. We also conducted a multiple group analysis (Italy and Sweden) using Structural Equation Modelling to investigate the relationship between all three subjective well-being constructs and psychological well-being. Italian adolescents scored significantly higher in satisfaction with life than Swedish adolescents. Additionally, across countries, girls scored significantly higher in negative affect than boys. In both countries, all three constructs of subjective well-being were significantly associated to adolescents' psychological well-being. Nevertheless, while the effect of the relationship between affect and psychological well-being was almost the same across countries, life satisfaction was more strongly related to psychological well-being among Swedish adolescents. The present study shows that there are larger variations between these two cultures in the cognitive construct of subjective well-being than in the affective construct. Accordingly, associations between the cognitive component, not the affective component, of subjective well-being and psychological well-being differ between countries as well.

  14. Scalable cell alignment on optical media substrates.

    PubMed

    Anene-Nzelu, Chukwuemeka G; Choudhury, Deepak; Li, Huipeng; Fraiszudeen, Azmall; Peh, Kah-Yim; Toh, Yi-Chin; Ng, Sum Huan; Leo, Hwa Liang; Yu, Hanry

    2013-07-01

    Cell alignment by underlying topographical cues has been shown to affect important biological processes such as differentiation and functional maturation in vitro. However, the routine use of cell culture substrates with micro- or nano-topographies, such as grooves, is currently hampered by the high cost and specialized facilities required to produce these substrates. Here we present cost-effective commercially available optical media as substrates for aligning cells in culture. These optical media, including CD-R, DVD-R and optical grating, allow different cell types to attach and grow well on them. The physical dimension of the grooves in these optical media allowed cells to be aligned in confluent cell culture with maximal cell-cell interaction and these cell alignment affect the morphology and differentiation of cardiac (H9C2), skeletal muscle (C2C12) and neuronal (PC12) cell lines. The optical media is amenable to various chemical modifications with fibronectin, laminin and gelatin for culturing different cell types. These low-cost commercially available optical media can serve as scalable substrates for research or drug safety screening applications in industry scales. Copyright © 2013 Elsevier Ltd. All rights reserved.

  15. Culture shapes empathic responses to physical and social pain.

    PubMed

    Atkins, David; Uskul, Ayse K; Cooper, Nicholas R

    2016-08-01

    The present research investigates the extent to which cultural background moderates empathy in response to observing someone undergoing physical or social pain. In 3 studies, we demonstrate that East Asian and White British participants differ in both affective and cognitive components of their empathic reactions in response to someone else's pain. Compared with East Asian participants, British participants report greater empathic concern and show lower empathic accuracy. More important, findings cannot be explained by an in-group advantage effect. Potential reasons for observed cultural differences are discussed. (PsycINFO Database Record (c) 2016 APA, all rights reserved).

  16. A multinational review of recent trends and reports in dementia caregiver burden.

    PubMed

    Torti, Frank M; Gwyther, Lisa P; Reed, Shelby D; Friedman, Joëlle Y; Schulman, Kevin A

    2004-01-01

    This systematic review of the literature focuses on the influence of ethnic, cultural, and geographic factors on the caregivers of patients with dementia. In particular, we explore the impact of cultural expectations on five important questions: 1) Do the characteristics of dementia affect caregiver burden? 2) Do characteristics of the caregiver independently predict burden? 3) Does the caregiver affect patient outcomes? 4) Does support or intervention for caregiver result in reduced caregiver burden or improved patient outcomes? 5) Finally, do patient interventions result in reduced caregiver burden or improved patient outcomes? Our findings suggest that noncognitive, behavioral disturbances of patients with dementia result in increased caregiver burden and that female caregivers bear a particularly heavy burden across cultures, particularly in Asian societies. Caregiver burden influences time to medical presentation of patients with dementia, patient condition at presentation, and patient institutionalization. Moreover, interventions designed to reduce caregiver burden have been largely, although not universally, unsuccessful. Pharmacological treatments for symptoms of dementia were found to be beneficial in reducing caregiver burden. The consistency of findings across studies, geographic regions, cultural differences, and heathcare delivery systems is striking. Yet, there are critical differences in cultural expectations and social resources. Future interventions to reduce caregiver burden must consider these differences, identify patients and caregivers at greatest risk, and develop targeted programs that combine aspects of a number of interventional strategies.

  17. [Blood stream infection and blood culture--"progress" and "blind" in blood culture testing].

    PubMed

    Kobayashi, Intetsu

    2005-04-01

    We have investigated various types of blood culture bottles which are mainly used at present and posed problems present in the blood culture bottles. First, there are differences between resin and ecosorb in the ability to adsorb and inactivate antibiotics in the blood. Second, the delay in placing the bottle (into which blood was inoculated) to the automatic instrument (delay in the start of incubation) greatly affects the automatic detection by BACTEC system and shows false negatives. Third, when the same blood is incubated in plural bottles (aerobic and anaerobic bottles), the differences among the detected organisms in the number are comparatively high, i.e., about 40%. In addition, there are differences among the organisms in the number of days required for the detection of the organisms. In this case, the detected organisms are clearly different in many cases. The technology of blood culture has been progressed remarkably. However, the efficiency of utilization of automatic instruments for diagnosis of infection depends greatly on the ability of laboratory technicians.

  18. Ecological Effects in Cross-Cultural Differences Between U.S. and Japanese Color Preferences.

    PubMed

    Yokosawa, Kazuhiko; Schloss, Karen B; Asano, Michiko; Palmer, Stephen E

    2016-09-01

    We investigated cultural differences between U.S. and Japanese color preferences and the ecological factors that might influence them. Japanese and U.S. color preferences have both similarities (e.g., peaks around blue, troughs around dark-yellow, and preferences for saturated colors) and differences (Japanese participants like darker colors less than U.S. participants do). Complex gender differences were also evident that did not conform to previously reported effects. Palmer and Schloss's (2010) weighted affective valence estimate (WAVE) procedure was used to test the Ecological Valence Theory's (EVT's) prediction that within-culture WAVE-preference correlations should be higher than between-culture WAVE-preference correlations. The results supported several, but not all, predictions. In the second experiment, we tested color preferences of Japanese-U.S. multicultural participants who could read and speak both Japanese and English. Multicultural color preferences were intermediate between U.S. and Japanese preferences, consistent with the hypothesis that culturally specific personal experiences during one's lifetime influence color preferences. Copyright © 2015 Cognitive Science Society, Inc.

  19. Eye contact perception in the West and East: a cross-cultural study.

    PubMed

    Uono, Shota; Hietanen, Jari K

    2015-01-01

    This study investigated whether eye contact perception differs in people with different cultural backgrounds. Finnish (European) and Japanese (East Asian) participants were asked to determine whether Finnish and Japanese neutral faces with various gaze directions were looking at them. Further, participants rated the face stimuli for emotion and other affect-related dimensions. The results indicated that Finnish viewers had a smaller bias toward judging slightly averted gazes as directed at them when judging Finnish rather than Japanese faces, while the bias of Japanese viewers did not differ between faces from their own and other cultural backgrounds. This may be explained by Westerners experiencing more eye contact in their daily life leading to larger visual experience of gaze perception generally, and to more accurate perception of eye contact with people from their own cultural background particularly. The results also revealed cultural differences in the perception of emotion from neutral faces that could also contribute to the bias in eye contact perception.

  20. Who I am depends on how I feel: the role of affect in the expression of culture.

    PubMed

    Ashton-James, Claire E; Maddux, William W; Galinsky, Adam D; Chartrand, Tanya L

    2009-03-01

    We present a novel role of affect in the expression of culture. Four experiments tested whether individuals' affective states moderate the expression of culturally normative cognitions and behaviors. We consistently found that value expressions, self-construals, and behaviors were less consistent with cultural norms when individuals were experiencing positive rather than negative affect. Positive affect allowed individuals to explore novel thoughts and behaviors that departed from cultural constraints, whereas negative affect bound people to cultural norms. As a result, when Westerners experienced positive rather than negative affect, they valued self-expression less, showed a greater preference for objects that reflected conformity, viewed the self in more interdependent terms, and sat closer to other people. East Asians showed the reverse pattern for each of these measures, valuing and expressing individuality and independence more when experiencing positive than when experiencing negative affect. The results suggest that affect serves an important functional purpose of attuning individuals more or less closely to their cultural heritage.

  1. Self-perceptions of cultural competence among dental students and recent graduates.

    PubMed

    Aleksejuniene, Jolanta; Zed, Chris; Marino, Rodrigo

    2014-03-01

    This study assessed self-perceptions of cultural competence in dental students and recent graduates of the University of British Columbia. The sample consisted of 106 predoctoral students (response rate 98 percent) and thirty-three recent graduates (response rate 43 percent). The two cohorts completed similar questionnaires. Over 80 percent of responding predoctoral students reported encountering patients from culturally different groups, 50 percent of them admitted that their communication is not effective, two-thirds were not confident in caring for patients from diverse cultural groups, and over 60 percent perceived that sociocultural differences affect the provision of care. Some significant differences between the genders and study years were observed. Exploratory Factor Analyses validated multiple indicators in five domains: 1) encountering culturally diverse patients, 2) communication challenges in sociocultural situations, 3) cultural competence-related skills, 4) cultural competence related to diagnosis and patient treatment, and 5) training in cultural competence. Through qualitative assessments, important culturally relevant topics and interactive training methods preferred by students for developing cultural competence were identified. This study concluded that cultural competence was perceived as important by both dental students and recent graduates but also as partly deficient, particularly by predoctoral students. For teaching cultural competence, participants recommended various topics and interactive teaching modalities.

  2. Health-related quality of life of irritable bowel syndrome patients in different cultural settings

    PubMed Central

    Faresjö, Åshild; Anastasiou, Foteini; Lionis, Christos; Johansson, Saga; Wallander, Mari-Ann; Faresjö, Tomas

    2006-01-01

    Background Persons with Irritable bowel syndrome (IBS) are seriously affected in their everyday life. The effect across different cultural settings of IBS on their quality of life has been little studied. The aim was to compare health-related quality of life (HRQOL) of individuals suffering from IBS in two different cultural settings; Crete, Greece and Linköping, Sweden. Methods This study is a sex and age-matched case-control study, with n = 30 Cretan IBS cases and n = 90 Swedish IBS cases and a Swedish control group (n = 300) randomly selected from the general population. Health-related quality of life, measured by SF-36 and demographics, life style indicators and co-morbidity, was measured. Results Cretan IBS cases reported lower HRQOL on most dimensions of SF-36 in comparison to the Swedish IBS cases. Significant differences were found for the dimensions mental health (p < 0.0001) and general health (p = 0.05) even after adjustments for educational level and co-morbidity. Women from Crete with IBS scored especially low on the dimensions general health (p = 0.009) and mental health (p < 0.0001) in comparison with Swedish women with IBS. The IBS cases, from both sites, reported significantly lower scores on all HRQOL dimensions in comparison with the Swedish control group. Conclusion The results from this study tentatively support that the claim that similar individuals having the same disease, e.g. IBS, but living in different cultural environments could perceive their disease differently and that the disease might affect their everyday life and quality of life in a different way. The Cretan population, and especially women, are more seriously affected mentally by their disease than Swedish IBS cases. Coping with IBS in everyday life might be more problematic in the Cretan environment than in the Swedish setting. PMID:16566821

  3. Reflections on the differences between religion and culture.

    PubMed

    Bonney, Richard

    2004-01-01

    Culture may be thought of as a causal agent that affects the evolutionary process by uniquely human means. Religion, on the other hand, is considered a process of revelation and contains the concept of the "faithful" who receive the message of revelation. Culture permits the "self-conscious evaluation of human possibilities" and therefore presents a device for increasing human control over species change. There are dangers, however, in accepting cultural relativism without any constraint, such as respect for human life and dignity. In this article, the author attempts to clarify the boundaries between religion and culture and acknowledges that further research is needed on the religion/culture dichotomy.

  4. Parenting and globalization in western countries: explaining differences in parent-child interactions.

    PubMed

    Prevoo, Mariëlle Jl; Tamis-LeMonda, Catherine S

    2017-06-01

    We review research on intra-cultural differences in parenting, and the sources of those differences. Ethnic-minority parents differ from majority parents in parenting values, childrearing goals and resources-differences that affect parenting practices and children's development. Within-country comparisons indicate less sensitivity, more authoritarian discipline, less child-focused communications, and less engagement in learning activities in ethnic-minority compared to ethnic-majority parents, which help account for disparities in children. Despite group differences in parenting, associations between parenting and child development generalize across cultures, with rare exceptions. However, a focus on intra-cultural differences is based on comparisons of group 'averages', which masks the enormous variation within ethnic-minority samples. Within-group variation can be partly explained by stressors associated with low socioeconomic status (SES), acculturation and discrimination. Copyright © 2017 Elsevier Ltd. All rights reserved.

  5. Exploring the Relationship of Organizational Culture and Implicit Leadership Theory to Performance Differences in the Nuclear and Fossil Energy Industry

    NASA Astrophysics Data System (ADS)

    Cravey, Kristopher J.

    Notable performance differences exist between nuclear and fossil power generation plants in areas such as safety, outage duration efficiency, and capacity factor. This study explored the relationship of organizational culture and implicit leadership theory to these performance differences. A mixed methods approach consisting of quantitative instruments, namely the Organizational Culture Assessment Instrument and the GLOBE Leadership Scales, and qualitative interviews were used in this study. Subjects were operations middle managers in a U.S. energy company that serves nuclear or fossil power plants. Results from the quantitative instruments revealed no differences between nuclear and fossil groups in regards to organizational culture types and implicit leadership theories. However, the qualitative results did reveal divergence between the two groups in regards to what is valued in the organization and how that drives behaviors and decision making. These organizational phenomenological differences seem to explain why performance differences exist between nuclear and fossil plants because, ultimately, they affect how the organization functions.

  6. [Effect of suspended silt from dredging at Yangtze estuary on Brachionus plicatilis].

    PubMed

    Wang, Jinqui; Xu, Zhaoli; Shi, Chun; Chen, Yaqu

    2002-07-01

    The effect of suspended silt from dredging at Yangtze estuary on Brachionus plicatilis was investigated by clonal culture (to construct life table) and population accumulative culture. The intrinsic increasing rate of the rotifer population was greatly reduced under different concentrations (1, 3, 5, 7 and 9 mg.ml-1) of silt, from 29.6% to 64.1%, and to a maximum of 130.0%. The suspended silt affected population survival rate, rather than its reproduction rate. In accumulative culture, the densities of female, males, parthenogenetic eggs, and resting eggs in the population were not affected by silt. It was concluded that the presence of suspended silt should have a certain negative influence on the rotifer population in dredging Yangtze estuary.

  7. Supplementing glycosylation: A review of applying nucleotide-sugar precursors to growth medium to affect therapeutic recombinant protein glycoform distributions.

    PubMed

    Blondeel, Eric J M; Aucoin, Marc G

    2018-06-15

    Glycosylation is a critical quality attribute (CQA) of many therapeutic proteins, particularly monoclonal antibodies (mAbs), and is a major consideration in the approval of biosimilar biologics due to its effects to therapeutic efficacy. Glycosylation generates a distribution of glycoforms, resulting in glycoproteins with inherent molecule-to-molecule heterogeneity, capable of activating (or failing to activate) different effector functions of the immune system. Glycoforms can be affected by the supplementation of nucleotide-sugar precursors, and related components, to culture growth medium, affecting the metabolism of glycosylation. These supplementations has been demonstrated to increase nucleotide-sugar intracellular pools, and impact glycoform distributions, but with varied results. These variations can be attributed to five key factors: Differences between cell platforms (enzyme/transporter expression levels); differences between recombinant proteins produced (glycan-site accessibility); the fermentation and sampling timeline (glucose availability and exoglycosidase accumulation); glutamine levels (affecting ammonia levels, which impact Golgi pH, as well as UDP-GlcNAc pools); and finally, a lack of standardized metrics for observing shifts in glycoform distributions (glycosylation indices) across different experiments. The purpose of this review is to provide detail and clarity on the state of the art of supplementation strategies for nucleotide-sugar precursors for affecting glycosylation in cell culture processes, and to apply glycosylation indices for standardized comparisons across the field. Copyright © 2018. Published by Elsevier Inc.

  8. Differences in gene expression profiles between human preimplantation embryos cultured in two different IVF culture media.

    PubMed

    Kleijkers, Sander H M; Eijssen, Lars M T; Coonen, Edith; Derhaag, Josien G; Mantikou, Eleni; Jonker, Martijs J; Mastenbroek, Sebastiaan; Repping, Sjoerd; Evers, Johannes L H; Dumoulin, John C M; van Montfoort, Aafke P A

    2015-10-01

    Is gene expression in human preimplantation embryos affected by the medium used for embryo culture in vitro during an IVF treatment? Six days of in vitro culture of human preimplantation embryos resulted in medium-dependent differences in expression level of genes involved in apoptosis, protein degradation, metabolism and cell-cycle regulation. Several human studies have shown an effect of culture medium on embryo development, pregnancy outcome and birthweight. However, the underlying mechanisms in human embryos are still unknown. In animal models of human development, it has been demonstrated that culture of preimplantation embryos in vitro affects gene expression. In humans, it has been found that culture medium affects gene expression of cryopreserved embryos that, after thawing, were cultured in two different media for 2 more days. In a multicenter trial, women were randomly assigned to two culture medium groups [G5 and human tubal fluid (HTF)]. Data on embryonic development were collected for all embryos. In one center, embryos originating from two pronuclei (2PN) zygotes that were not selected for transfer or cryopreservation on Day 2 or 3 because of lower morphological quality, were cultured until Day 6 and used in this study, if couples consented. Ten blastocysts each from the G5 and HTF study groups, matched for fertilization method, maternal age and blastocyst quality, were selected and their mRNA was isolated and amplified. Embryos were examined individually for genome-wide gene expression using Agilent microarrays and PathVisio was used to identify the pathways that showed a culture medium-dependent activity. Expression of 951 genes differed significantly (P < 0.01) between the G5 and HTF groups. Eighteen pathways, involved in apoptosis, metabolism, protein processing and cell-cycle regulation, showed a significant overrepresentation of differentially expressed genes. The DNA replication, G1 to S cell-cycle control and oxidative phosphorylation pathways were up-regulated in the G5 group compared with the HTF group. This is in agreement with the morphological assessment of the 1527 embryos (originating from 2PN zygotes), which showed that embryos consisted of more cells on Day 2 (3.73 ± 1.30 versus 3.40 ± 1.35, P < 0.001) and Day 3 (7.00 ± 2.41 versus 5.84 ± 2.36, P < 0.001) in the G5 group when compared with the HTF group. Furthermore, the implantation rate was significantly higher in the G5 group compared with the HTF group (26.7% versus 14.7%, P = 0.002) after transfer on the second or the third day after fertilization. Despite careful matching of the embryos, it cannot be excluded that the differences observed between the study groups are caused by factors that we did not investigate. Extrapolation of these results to embryos used for transfer demands caution as in the present study embryos that were not selected for either embryo transfer or cryopreservation have been used for the culture experiment until Day 6. This study shows that gene expression in human preimplantation embryos is altered by the culture medium used during IVF treatment and provides insight into the biological pathways that are affected. Whether these changes in gene expression have any long-term effects on children born after IVF remains unknown. However, it is possible that early adaptations of the preimplantation embryo to its environment persist during fetal and post-natal development. No funding and no competing interests declared. Not applicable. © The Author 2015. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  9. Thinking Differently about "Difference": Multicultural Literature and Service-Learning

    ERIC Educational Resources Information Center

    Grobman, Laurie

    2004-01-01

    Questions of "difference," embroiled in identity politics, a politics of difference, cultural identity(ies) and democracy, are among multicultural literary studies' most pressing and controversial issues and affect all who teach literature by writers of multicultural backgrounds, especially in introductory and survey courses. In this article, the…

  10. Growth and morphogenesis of embryonic mouse organs on non-coated and extracellular matrix-coated Biopore membrane

    NASA Technical Reports Server (NTRS)

    Hardman, P.; Klement, B. J.; Spooner, B. S.

    1993-01-01

    Embryonic mouse salivary glands, pancreata, and kidneys were isolated from embryos of appropriate gestational age by microdissection, and were cultured on Biopore membrane either non-coated or coated with type I collagen or Matrigel. As expected, use of Biopore membrane allowed high quality photomicroscopy of the living organs. In all organs extensive mesenchymal spreading was observed in the presence of type I collagen or Matrigel. However, differences were noted in the effects of extracellular matrix (ECM) coatings on epithelial growth and morphogenesis: salivary glands were minimally affected, pancreas morphogenesis was adversely affected, and kidney growth and branching apparently was enhanced. It is suggested that these differences in behaviour reflect differences in the strength of interactions between the mesenchymal cells and their surrounding endogenous matrix, compared to the exogenous ECM macromolecules. This method will be useful for culture of these and other embryonic organs. In particular, culture of kidney rudiments on ECM-coated Biopore offers a great improvement over previously used methods which do not allow morphogenesis to be followed in vitro.

  11. Affective Components Perceived to Be Important in Today's Global Society from a Cross-Cultural Perspective

    ERIC Educational Resources Information Center

    Wallenberg-Lerner, Helena

    2013-01-01

    Global competencies, with differences in terminology by various researchers, had been frequently investigated, primarily from an American-biased perspective. Little or no defining research existed that identified requisite, universally agreed upon global competencies, or identified what affective components were perceived to be important cross…

  12. Empowerment of Refugees by Language: Can ESL Learners Affect the Target Culture?

    ERIC Educational Resources Information Center

    Tadayon, Fereshteh; Khodi, Ali

    2016-01-01

    Numerous studies have investigated the changing patterns of immigration, the growth of multicultural-multilingual societies, and the important role of language in identity construction. Unfortunately, the issue of identity construction is affected by a variety of factors such as language learning and acquisition underlying different contexts and…

  13. Light as an Energy Source in Continuous Cultures of Bacteriorhodopsin-Containing Halobacteria

    PubMed Central

    Rodriguez-Valera, F.; Nieto, J. J.; Ruiz-Berraquero, F.

    1983-01-01

    The role of light as an energy source for slightly aereated cultures of halobacteria was studied, using continuous cultures with low nutrient concentrations and a low oxygen supply. A series of experiments were carried out with non-illuminated and differently illuminated cultures and with different oxygen transfer rates. Under low oxygen availability, light proved to be a decisively important energy source that allowed the populations to reach higher growth rates and much higher population densities. Oxygen influenced the growth over only a minimal level, below which neither the illuminated nor the dark cultures were affected by the oxygen transfer rate. From these results, it appears that the bacteriorhodopsin-mediated energy supply could have a very important role for the ecology of halobacteria in their microaerophilic habitats. In the illuminated cultures, cells that originated purple colonies on plates appeared. These cells, which could be bacteriorhodopsin-constitutive mutants, are now being studied. PMID:16346250

  14. Cultural differences and shame in an expressive writing alcohol intervention.

    PubMed

    Rodriguez, Lindsey M; Young, Chelsie M; Neighbors, Clayton; Tou, Reese; Lu, Qian

    2016-01-01

    The present study evaluates the relationships between shame, culture, and drinking behavior in predicting drinking intentions in the context of an expressive writing intervention. Theory and empirical findings have generally found that shame is maladaptive and can lead to anxiety, depression, and problematic alcohol use. However, research on cultural differences suggests that shame may be differentially damaging to individuals of collectivist, Asian cultures. Previous research evaluating expressive writing as a brief alcohol intervention has shown promising results such as reduced drinking intentions and increased readiness to change drinking behavior. The present study tested the hypothesis that feelings of shame after writing about a negative heavy drinking event would be associated with greater alcohol use generally and that this effect would differ for Caucasian compared to Asian individuals. We also explored whether this differed for light and heavy drinkers. Two hundred sixty-four undergraduates (74% female) who drank at least one alcoholic beverage in the past month completed measures of demographics, baseline drinking, event-related shame and guilt, pre- and postwriting affect, and drinking intentions. Results revealed that, independent of affect, social desirability, and event-related guilt, shame was generally negatively associated with drinking intentions for Caucasians and light drinking Asians. However, for heavy drinking Asians, shame was associated with increased drinking intentions. Results suggest that interventions that elicit shame are differentially effective and should be targeted accordingly.

  15. FMRI investigation of cross-cultural music comprehension.

    PubMed

    Morrison, Steven J; Demorest, Steven M; Aylward, Elizabeth H; Cramer, Steven C; Maravilla, Kenneth R

    2003-09-01

    The popular view of music as a "universal" language ignores the privileged position of the cultural insider in comprehending musical information unique to their own tradition. The purpose of this study was to test the hypothesis that listeners would demonstrate different neural activity in response to culturally familiar and unfamiliar music and that those differences may be affected by the extent of subjects' formal musical training. Just as familiar languages have been shown to use distinct brain processes, we hypothesized that an analogous difference might be found in music and that it may depend in part on subjects' formal musical knowledge. Using fMRI we compared the activation patterns of professional musicians and untrained controls reared in the United States as they listened to music from their culture (Western) and from an unfamiliar culture (Chinese). No overall differences in activation were observed for either subject group in response to the two musical styles, although there were differences in recall performance based on style and there were activation differences based on training. Trained listeners demonstrated additional activation in the right STG for both musics and in the right and left midfrontal regions for Western music and Chinese music, respectively. Our findings indicate that listening to culturally different musics may activate similar neural resources but with dissimilar results in recall performance.

  16. Further evidence that culture media affect perinatal outcome: findings after transfer of fresh and cryopreserved embryos.

    PubMed

    Nelissen, Ewka C; Van Montfoort, Aafke P; Coonen, Edith; Derhaag, Josien G; Geraedts, Joep P; Smits, Luc J; Land, Jolande A; Evers, Johannes L; Dumoulin, John C

    2012-07-01

    We have previously shown that the medium used for culturing IVF embryos affects the birthweight of the resulting newborns. This observation with potentially far-reaching clinical consequences during later life, was made in singletons conceived during the first IVF treatment cycle after the transfer of fresh embryos. In the present study, we hypothesize that in vitro culture of embryos during the first few days of preimplantation development affects perinatal outcome, not only in singletons conceived in all rank order cycles but also in twins and in children born after transfer of frozen embryos. Furthermore, we investigated the effect of culture medium on gestational age (GA) at birth. Oocytes and embryos from consecutive treatment cycles were alternately assigned to culture in either medium from Vitrolife or from Cook. Data on a cohort of 294 live born singletons conceived after fresh transfer during any of a patient's IVF treatment cycles, as well as data of 67 singletons conceived after frozen embryo transfer (FET) and of 88 children of 44 twin pregnancies after fresh transfer were analysed by means of multiple linear regression. In vitro culture in medium from Cook resulted in singletons after fresh transfer with a lower mean birthweight (adjusted mean difference, 112 g, P= 0.03), and in more singletons with low birthweight (LBW) <2500 g (P= 0.006) and LBW for GA ≥ 37 weeks (P= 0.015), when compared with singletons born after culture in medium from Vitrolife AB. GA at birth was not related to the medium used (adjusted difference, 0.05 weeks, P = 0.83). Among twins in the Cook group, higher inter-twin mean birthweight disparity and birthweight discordance were found. Z-scores after FET were -0.04 (± 0.14) in the Cook group compared with 0.18 (± 0.21) in the Vitrolife group (P> 0.05). Our findings support our hypothesis that culture medium influences perinatal outcome of IVF singletons and twins. A similar trend is seen in case of singletons born after FET. GA was not affected by culture medium. These results indicate that in vitro culture might be an important factor explaining the poorer perinatal outcome after assisted reproduction technology (ART). Further research is needed to confirm this culture medium-induced effect in humans and to provide more insight into whether it is caused by epigenetic disturbance of imprinted genes in fetal or placental tissues. Moreover, embryo culture media and their effects need to be investigated thoroughly to select the best embryo culture medium in order to minimize or prevent short-term risks and maybe even long-term disease susceptibility.

  17. A Study in Difference: Structures and Cultures in Australian Registered Training Organisations. Full Report

    ERIC Educational Resources Information Center

    Clayton, Berwyn; Fisher, Thea; Harris, Roger; Bateman, Andrea; Brown, Mike

    2008-01-01

    This report presents the findings of a study examining organisational culture and structure in ten Australian registered training organisations (RTOs) and is part of a program of research examining the factors which affect and help build the capability of vocational education and training (VET) providers. The study sought to determine: (1) how…

  18. The Effect of School-Based Management on Schools' Culture of Consumption

    ERIC Educational Resources Information Center

    Nir, Adam E.

    2007-01-01

    The purpose of this study was to assess the extent to which the introduction of school-based management (SBM) has affected schools' culture of consumption and the inequalities between schools with different socio-economic backgrounds. An analysis of financial reports from 31 SBM schools over four years reveals that schools have increased rather…

  19. Maternal Socialization of Children's Anger, Sadness, and Physical Pain in Two Communities in Gujarat, India

    ERIC Educational Resources Information Center

    Raval, Vaishali Vidhatri; Martini, Tanya Susan

    2009-01-01

    Despite the recognition of cultural influences in child socialization, little is known about socialization of emotion in children from different cultures. This study examined (a) Gujarati Indian mothers' reports concerning their beliefs, affective and behavioral responses to their children's displays of anger, sadness, and physical pain, and (b)…

  20. Invited Reaction: Factors Affecting Cross-Cultural Adjustment--Training, Experience, and Individual Differences

    ERIC Educational Resources Information Center

    Budworth, Marie-Helene; DeGama, Nadia

    2012-01-01

    In today's global marketplace, it is critical that one develops and prepares employees for working across borders in a range of cultural contexts. The organization's ability to compete will be predicated on the ability of its people to lead, manage, negotiate, and resolve conflict with clients, colleagues, and business partners around the world.…

  1. Corporal Punishment, Discipline and Cultural Differences and Expectations.

    ERIC Educational Resources Information Center

    Jones, Johnny L.

    Schools can no longer be responsible for only the cognitive domain of the child. They must also expand to include the affective domain. When it comes to the corporal punishment or discipline of a disruptive black child, the child's cultural history and history of alienation must be considered. And, because it is the teacher who controls rewards…

  2. Effects of Culture and Gender in Comprehension of Speech Acts of Indirect Request

    ERIC Educational Resources Information Center

    Shams, Rabe'a; Afghari, Akbar

    2011-01-01

    This study investigates the comprehension of indirect request speech act used by Iranian people in daily communication. The study is an attempt to find out whether different cultural backgrounds and the gender of the speakers affect the comprehension of the indirect request of speech act. The sample includes thirty males and females in Gachsaran…

  3. YouTube Acceptance by University Educators and Students: A Cross-Cultural Perspective

    ERIC Educational Resources Information Center

    Jung, Insung; Lee, Yekyung

    2015-01-01

    Despite the huge popularity of YouTube, there has been little research into the factors affecting educational applications of this social medium. This study attempts to predict and compare factors influencing YouTube acceptance among university students and educators in two very different cultures, Japan and the USA, applying the Unified Theory of…

  4. Beyond Economy: Impact of National Cultural Values on Nationwide Broadband Internet Diffusion

    ERIC Educational Resources Information Center

    Joo, Sung-Hee

    2010-01-01

    This study examines how the non-conventional factors of national cultural values and government involvement affect the diffusion of broadband Internet technologies in various nations around the world. An innovative element of the study was the examination of the influence patterns at different stages of diffusion, which was measured by the number…

  5. Cross/Intercultural Training. A One-Day Seminar in Preparation of Travel Abroad

    ERIC Educational Resources Information Center

    Sierra, Juan Jose Martinez

    2008-01-01

    Globalization is affecting people in ways they are often unaware of. The most visible aspect is perhaps economic, but there are other elements flowing from the dominant cultures (particularly the American) to the rest of the world that influence cultural features via different means (e.g. cinema or television). A further effect of the highly…

  6. Violent video game effects on aggression, empathy, and prosocial behavior in eastern and western countries: a meta-analytic review.

    PubMed

    Anderson, Craig A; Shibuya, Akiko; Ihori, Nobuko; Swing, Edward L; Bushman, Brad J; Sakamoto, Akira; Rothstein, Hannah R; Saleem, Muniba

    2010-03-01

    Meta-analytic procedures were used to test the effects of violent video games on aggressive behavior, aggressive cognition, aggressive affect, physiological arousal, empathy/desensitization, and prosocial behavior. Unique features of this meta-analytic review include (a) more restrictive methodological quality inclusion criteria than in past meta-analyses; (b) cross-cultural comparisons; (c) longitudinal studies for all outcomes except physiological arousal; (d) conservative statistical controls; (e) multiple moderator analyses; and (f) sensitivity analyses. Social-cognitive models and cultural differences between Japan and Western countries were used to generate theory-based predictions. Meta-analyses yielded significant effects for all 6 outcome variables. The pattern of results for different outcomes and research designs (experimental, cross-sectional, longitudinal) fit theoretical predictions well. The evidence strongly suggests that exposure to violent video games is a causal risk factor for increased aggressive behavior, aggressive cognition, and aggressive affect and for decreased empathy and prosocial behavior. Moderator analyses revealed significant research design effects, weak evidence of cultural differences in susceptibility and type of measurement effects, and no evidence of sex differences in susceptibility. Results of various sensitivity analyses revealed these effects to be robust, with little evidence of selection (publication) bias.

  7. The influence of culture on maternal soothing behaviours and infant pain expression in the immunization context

    PubMed Central

    Vinall, Jillian; Pillai Riddell, Rebecca; Greenberg, Saul

    2011-01-01

    OBJECTIVE: To investigate how maternal culture (ie, individualist versus collectivist) influences soothing techniques and infant distress. METHODS: Archival data were analyzed using a subsample of 80 mother-infant dyads selected from a larger database of infant pain expression. RESULTS: Mothers belonging to the individualist group used more affection behaviours when attempting to regulate their infants’ distress. No differences were observed in mothers’ touching, holding, rocking, vocalizing, caregiving or distracting their infants. Mothers’ culture did not appear to be related to the level of distress expressed by their infants. CONCLUSIONS: These results suggest that the similarities in soothing and infant pain expression between individualist and collectivist cultures are more prominent than their differences. PMID:22059192

  8. Ostracism and attachment orientation: Avoidants are less affected in both individualistic and collectivistic cultures.

    PubMed

    Yaakobi, Erez; Williams, Kipling D

    2016-03-01

    Ostracism--being excluded and ignored--is painful and threatens needs for belonging, self-esteem, control and meaningful existence. Many studies have shown that immediate responses to ostracism tend to be resistant to moderation. Once ostracized individuals are able to reflect on the experience, however, personality and situational factors moderate recovery speed and behavioural responses. Because attachment orientation is grounded in perceptions of belonging, we hypothesized that attachment orientation would moderate both immediate and delayed reactions to laboratory-induced ostracism. Participants from individualistic or collectivistic cultures were either included or ostracized in a game of Cyberball, a virtual ball-toss game. In both cultures, we found that compared to more securely attached individuals, more avoidant participants were less distressed by ostracism, but more distressed by inclusion. It is suggested that over and beyond differences in culture, individuals who avoid meaningful attachment may be less affected by ostracism. © 2015 The British Psychological Society.

  9. Embryo density may affect embryo quality during in vitro culture in a microwell group culture dish.

    PubMed

    Lehner, Adam; Kaszas, Zita; Murber, Akos; Rigo, Janos; Urbancsek, Janos; Fancsovits, Peter

    2017-08-01

    Culturing embryos in groups is a common practice in mammalian embryology. Since the introduction of different microwell dishes, it is possible to identify oocytes or embryos individually. As embryo density (embryo-to-volume ratio) may affect the development and viability of the embryos, the purpose of this study was to assess the effect of different embryo densities on embryo quality. Data of 1337 embryos from 228 in vitro fertilization treatment cycles were retrospectively analyzed. Embryos were cultured in a 25 μl microdrop in a microwell group culture dish containing 9 microwells. Three density groups were defined: Group 1 with 2-4 (6.3-12.5 μl/embryo), Group 2 with 5-6 (4.2-5.0 μl/embryo), and Group 3 with 7-9 (2.8-3.6 μl/embryo) embryos. Proportion of good quality embryos was higher in Group 2 on both days (D2: 18.9 vs. 31.5 vs. 24.7%; p < 0.001; D3: 19.7 vs. 27.1 vs. 21.2%; p = 0.029; Group 1. vs. Group 2. vs. Group 3). Cell number on Day 3 differed between Groups 1 and 2 (6.8 ± 2.2; 7.3 ± 2.1; p = 0.004) and Groups 2 and 3 (7.3 ± 2.1 vs. 7.0 ± 2.0; p = 0.014). Culturing 5-6 embryos together in a culture volume of 25 μl may benefit embryo quality. As low egg number, position, and distance of the embryos may influence embryo quality, results should be interpreted with caution.

  10. Looking across three generations of Alaska Natives to explore how culture fosters indigenous resilience.

    PubMed

    Wexler, Lisa

    2014-02-01

    Research has established connection between indigenous culture--often described in terms of cultural identity, enculturation, and participation in traditional activities--and resilience, the process by which people overcome acute and ongoing challenges. Despite correlations between culture and resilience, research has seldom described the ways these concepts are linked in indigenous people's narratives. Furthermore, little attention has been paid to the affect of historical trauma on different generations' understanding and deployment of "culture" in the context of hardship. This project, conducted in the summer of 2008 in an indigenous Arctic community, focuses on narratives from three generations who have experienced different degrees of cultural suppression in their lifetimes. From this starting point, the study explores how individuals make meaning and take strength from particular notions of culture, and illuminates the ways each generation accesses and deploys their cultural understandings in the face of hardship. By identifying the similarities and differences in both the challenges and sources of strength for each generation, the paper highlights how understandings of culture are shaped by historical experiences and modified through time. The differing ways that culture fosters strength, purpose, and fortitude (or does not) in indigenous young people's, adults' and Elders' life stories provide clues for enhancing indigenous youth resilience. Findings suggest that "culture" can galvanize Inupiaq people's sense of identity, feeling of commitment, and purpose, all of which are protective. However, young people need support in developing particular ideas around cultural identity and group membership that can contribute to resilience.

  11. Phytotoxicity, uptake and metabolism of 1,4-dichlorobenzene by plant cells

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Wang, M.J.; Bokern, M.; Boehme, C.

    1996-07-01

    Phytotoxicity, uptake, and metabolism of 1,4-dichlorobenzene (1,4-DCB) by carrot (Daucus carota L.), soybean (Glycine max. L.), tomato (Lycopersicon esculentum Mill.), and red goosefoot (Chenopodiun rubrum L.) cell suspension cultures were studied. Sealed glass systems were utilized for the investigation because 1,4-DCB is volatile. The sealed systems affect the growth of plant cells, but do not provide different results when testing xenobiotic uptake and metabolism. 1,4-Dichlorobenzene (40 {micro}g in 40 ml medium) was taken up by carrot (49%), soybean (50%), and red goosefoot (62%) cells. Only the soybean cell cultures provided evidence of the existence of metabolites of this compound, probablymore » conjugates of chlorophenols. Conditions for phytotoxicity tests were modified because the growth of cell cultures was affected when sealed for longer than 2 d. 1,4-Dichlorobenzene is toxic to cell cultures of the three tested plant species (tomato, soybean, and carrot). Concentrations of 0.5 mM caused 50% growth inhibition in carrot and soybean cultures. The tomato cultures were more sensitive, with 0.05 mM causing 50% growth inhibition.« less

  12. How does culture affect experiential training feedback in exported Canadian health professional curricula?

    PubMed Central

    Mousa Bacha, Rasha; Abdelaziz, Somaia

    2017-01-01

    Objectives To explore feedback processes of Western-based health professional student training curricula conducted in an Arab clinical teaching setting. Methods This qualitative study employed document analysis of in-training evaluation reports (ITERs) used by Canadian nursing, pharmacy, respiratory therapy, paramedic, dental hygiene, and pharmacy technician programs established in Qatar. Six experiential training program coordinators were interviewed between February and May 2016 to explore how national cultural differences are perceived to affect feedback processes between students and clinical supervisors. Interviews were recorded, transcribed, and coded according to a priori cultural themes. Results Document analysis found all programs’ ITERs outlined competency items for students to achieve. Clinical supervisors choose a response option corresponding to their judgment of student performance and may provide additional written feedback in spaces provided. Only one program required formal face-to-face feedback exchange between students and clinical supervisors. Experiential training program coordinators identified that no ITER was expressly culturally adapted, although in some instances, modifications were made for differences in scopes of practice between Canada and Qatar.  Power distance was recognized by all coordinators who also identified both student and supervisor reluctance to document potentially negative feedback in ITERs. Instances of collectivism were described as more lenient student assessment by clinical supervisors of the same cultural background. Uncertainty avoidance did not appear to impact feedback processes. Conclusions Our findings suggest that differences in specific cultural dimensions between Qatar and Canada have implications on the feedback process in experiential training which may be addressed through simple measures to accommodate communication preferences. PMID:28315858

  13. How does culture affect experiential training feedback in exported Canadian health professional curricula?

    PubMed

    Wilbur, Kerry; Mousa Bacha, Rasha; Abdelaziz, Somaia

    2017-03-17

    To explore feedback processes of Western-based health professional student training curricula conducted in an Arab clinical teaching setting. This qualitative study employed document analysis of in-training evaluation reports (ITERs) used by Canadian nursing, pharmacy, respiratory therapy, paramedic, dental hygiene, and pharmacy technician programs established in Qatar. Six experiential training program coordinators were interviewed between February and May 2016 to explore how national cultural differences are perceived to affect feedback processes between students and clinical supervisors. Interviews were recorded, transcribed, and coded according to a priori cultural themes. Document analysis found all programs' ITERs outlined competency items for students to achieve. Clinical supervisors choose a response option corresponding to their judgment of student performance and may provide additional written feedback in spaces provided. Only one program required formal face-to-face feedback exchange between students and clinical supervisors. Experiential training program coordinators identified that no ITER was expressly culturally adapted, although in some instances, modifications were made for differences in scopes of practice between Canada and Qatar.  Power distance was recognized by all coordinators who also identified both student and supervisor reluctance to document potentially negative feedback in ITERs. Instances of collectivism were described as more lenient student assessment by clinical supervisors of the same cultural background. Uncertainty avoidance did not appear to impact feedback processes. Our findings suggest that differences in specific cultural dimensions between Qatar and Canada have implications on the feedback process in experiential training which may be addressed through simple measures to accommodate communication preferences.

  14. The experiences of Western expatriate nursing educators teaching in Eastern Asia.

    PubMed

    Melby, Carolyn S; Dodgson, Joan E; Tarrant, Marie

    2008-01-01

    To describe the lived experience of English-speaking Western nurse educators teaching in East Asian countries. The study design was an application of existential phenomenological approach to qualitative data collection and analysis. Eight expatriate nurse educators who had taught more than 2 years in an East Asian country were interviewed about their experiences between January 2004 and November 2005. Narrative data were analyzed using a phenomenological approach aimed at distilling the experience of the educators. Each member of the research team analyzed the data, then through group discussions a consensus was reached, paying close attention to developing clear understandings of language nuances and maintaining the participants' voices. Four themes emerged from the data: (a) differing expectations, (b) the cost of the expatriate experience, (c) bridging pedagogies, and (d) adapting and finding purpose. The tacit meanings of cultural differences affecting participants' experiences are presented. The ways that a collectivist-oriented culture may affect nurse educators coming from a Western individualist worldview needs to continue to be researched to develop better mutual understandings that will lead to culturally collaborative models of nursing practice, education and research. Nurses providing direct care and nurse educators who work with people from cultures other than their own will find the discussion of cross-cultural misunderstandings useful.

  15. Proteolytic and ACE-inhibitory activities of probiotic yogurt containing non-viable bacteria as affected by different levels of fat, inulin and starter culture.

    PubMed

    Shakerian, Mansour; Razavi, Seyed Hadi; Ziai, Seyed Ali; Khodaiyan, Faramarz; Yarmand, Mohammad Saeid; Moayedi, Ali

    2015-04-01

    In this study, the effects of fat (0.5 %, 3.2 % and 5.0 %), inulin (0.0 and 1.0 %) and starter culture (0.0 %, 0.5 %, 1.0 % and 1.5 %) on the angiotensin converting enzyme (ACE)-inhibitory activity of probiotic yogurt containing non-viable bacteria were assessed. Proteolytic activities of bacteria were also investigated. Yogurts were prepared either using a sole yogurt commercial culture including Streptococcus thermophilus and Lactobacillus delbrueckii subs. bulgaricus or bifidobacterium animalis BB-12 and Lactobacillus acidophilus La5 in addition to yogurt culture. Relative degrees of proteolysis were found to be considerably higher in yogurt samples than UHT milk as the control. Both regular and probiotic yogurts showed considerable ACE-inhibitory activities. Results showed that degree of proteolysis was not influenced by different fat contents, while was increased by high concentration of starter culture (1.5 % w/w) and reduced by inulin (1 % w/w). ACE-inhibitory activities of yogurt were also negatively affected by the presence of inulin and high levels of fat (5 % w/w). Moreover, yogurt containing probiotic bacteria showed higher inhibitory against ACE in comparison to the yogurt prepared with non-probiotic strains.

  16. Cultural factors and the International Space Station.

    PubMed

    Ritsher, Jennifer Boyd

    2005-06-01

    The American and Russian/Soviet space programs independently uncovered psychosocial risks inherent in long-duration space missions. Now that these two countries are working together on the International Space Station (ISS), American-Russian cultural differences pose an additional set of risk factors. These may echo cultural differences that have been observed in the general population of the two countries and in space analogue settings, but little is known about how relevant these are to the select population of space program personnel. The evidence for the existence of mission-relevant cultural differences is reviewed and includes cultural values, emotional expressivity, personal space norms, and personality characteristics. The review is focused primarily on Russia and the United States, but also includes other ISS partner countries. Cultural differences among space program personnel may have a wide range of effects. Moreover, culture-related strains may increase the probability of distress and impairment. Such factors could affect the individual and interpersonal functioning of both crewmembers and mission control personnel, whose performance is also critical for mission safety and success. Examples from the anecdotal and empirical literature are given to illustrate these points. The use of existing assessment strategies runs the risk of overlooking important early warning signs of behavioral health difficulties. By paying more attention to cultural differences and how they might be manifested, we are more likely to detect problems early while they are still mild and resolvable.

  17. Cultural and social determinants of health among indigenous Mexican migrants in the United States.

    PubMed

    Lee, Junghee; Donlan, William; Cardoso, Edgar Ezequiel Orea; Paz, Juan Jesus

    2013-01-01

    Despite growing numbers, indigenous Mexican migrants are relatively invisible to health practitioners who group them with nonindigenous, mestizo Mexican-origin populations. Associations between indigenous and mestizo cultural identifications with psychosocial characteristics and health indicators among indigenous Mexican migrants were examined. Results revealed gender differences in cultural identifications, perceived discrimination, self-esteem, self-efficacy, and various health indicators including depression severity, culture-bound syndromes, and self-rated health. Multivariate regression and structural equation path modeling demonstrated how indigenous cultural identification and perceived discrimination affects health. Findings suggest that interventions should utilize indigenous community-based activities designed to promote self-esteem and the value of indigenous culture, with a focus on females.

  18. A Multicountry Perspective on Cultural Competence Among Baccalaureate Nursing Students.

    PubMed

    Cruz, Jonas Preposi; Aguinaldo, Alexis Nacionales; Estacio, Joel Casuga; Alotaibi, Abdullelah; Arguvanli, Sibel; Cayaban, Arcalyd Rose Ramos; John Cecily, Helen Shaji; Machuca Contreras, Felipe Aliro; Hussein, Adraa; Idemudia, Erhabor Sunday; Mohamed, Shihab Aldeen Mourtada; Sebaeng, Jeanette

    2018-01-01

    To assess cultural competence among nursing students from nine countries to provide an international perspective on cultural competence. A descriptive, cross-sectional design. A convenience sample of 2,163 nursing students from nine countries was surveyed using the Cultural Capacity Scale from April to November 2016. The study found a moderate range of cultural competence among the students. The ability to teach and guide other nursing colleagues to display culturally appropriate behavior received the highest competence rating, while the ability to discuss differences between the client's health beliefs or behaviors and nursing knowledge with each client received the lowest competence rating. Differences in cultural competence were observed between students from different countries. Country of residence, gender, age, year of study, attendance at cultural-related training, the experience of taking care of patients from culturally diverse backgrounds and patients belonging to special population groups, and living in a multicultural environment were identified as factors affecting cultural competence. The international perspective of cultural competence among nursing students provided by this study serves as a vital preview of where nursing education currently stands in terms of providing the necessary preparatory competence in the cultural aspect of care. The variation of cultural competence among nursing students from different nations should serve as a cue for designing a focused yet multimodal nursing education program in guiding them to be culturally sensitive, culturally adaptive, and culturally motivated. The training of nursing students in providing competent culturally appropriate care should be ensured considering that adequate preparation of nursing students guarantees future competent nursing practice, which can positively impact the nursing profession in any part of the globe. © 2017 Sigma Theta Tau International.

  19. Cultural impacts on public perceptions of agricultural biotechnology: A comparison of South Korea and the United States

    NASA Astrophysics Data System (ADS)

    Nader, Richard Harrison

    According to Millar (1996), the gulf between science and society is growing. Technologies are tools cultures develop to solve society's problems. The rapid dispersion of science and technology across cultural borders through trade, technology transfer and exchange, increasingly requires people in different cultures to make choices about accepting or rejecting artifacts of science and technology such as genetically modified (GM) foods, which originate primarily from the United States. These issues challenge policy makers and scientists to account for the affects of different cultural perspectives on controversial scientific issues. Given the controversy across cultures over acceptance or rejection of genetically modified (GM) foods, GM foods are an excellent example with which to begin to reveal how culture impacts public perceptions of the risk and benefits of science and technology in different societies. This research will: (1) Define public awareness and understanding of science, specifically GM foods; (2) Examine culture's impact on knowledge, including different cultural approaches to research; and (3) Compare recent findings of a bi-national public opinion survey on GM comparing in South Korea and the United States. The proposed research outlines two research questions: (1) How and in what ways do South Koreans and Americans differ in their opinions about GMOs? This question is important for gathering current points of contrast about how the two cultures may differ; and (2) What role does culture play on opinion formation about GM foods? Through grounded theory, the researcher will investigate how cultural differences help explain opinion on public perceptions of GM foods. Is it possible to identify common cultural factors that impact public perceptions of GM foods between South Koreans and Americans? The study will utilize both qualitative and quantitative methodologies. Higher education is a major producer of new science and technology. The study is significant for higher education administrators who must understand cultural factors impacting science internationally and globalization of the academic enterprise.

  20. Korean working mothers' parenting style in Korea and in the United STates: a qualitative comparative study.

    PubMed

    Im, Hyesang; Kim, Eunjung; Sung, Kyungsuk

    2014-01-01

    The purpose of this study was to explore the similarities and differences of cultural influences on the parenting styles of Korean working mothers who live in South Korea versus Korean American working mothers living in the U.S. Four major themes were identified: (a) expression of affection for children, (b) parental control, (c) feelings for children, and (d) feelings for themselves. The findings indicate that acculturation to the American culture affected the Korean American working mothers to grant higher self-regulation to their children and to have more positive feelings for their children and themselves.

  1. The Differential Organization of F-Actin Alters the Distribution of Organelles in Cultured When Compared to Native Chromaffin Cells

    PubMed Central

    Gimenez-Molina, Yolanda; Villanueva, José; Nanclares, Carmen; Lopez-Font, Inmaculada; Viniegra, Salvador; Francés, Maria del Mar; Gandia, Luis; Gil, Amparo; Gutiérrez, Luis M.

    2017-01-01

    Cultured bovine chromaffin cells have been used extensively as a neuroendocrine model to study regulated secretion. In order to extend such experimental findings to the physiological situation, it is necessary to study mayor cellular structures affecting secretion in cultured cells with their counterparts present in the adrenomedullary tissue. F-actin concentrates in a peripheral ring in cultured cells, as witnessed by phalloidin–rodhamine labeling, while extends throughout the cytoplasm in native cells. This result is also confirmed when studying the localization of α-fodrin, a F-actin-associated protein. Furthermore, as a consequence of this redistribution of F-actin, we observed that chromaffin granules and mitochondria located into two different cortical and internal populations in cultured cells, whereas they are homogeneously distributed throughout the cytoplasm in the adrenomedullary tissue. Nevertheless, secretion from isolated cells and adrenal gland pieces is remarkably similar when measured by amperometry. Finally, we generate mathematical models to consider how the distribution of organelles affects the secretory kinetics of intact and cultured cells. Our results imply that we have to consider F-actin structural changes to interpret functional data obtained in cultured neuroendocrine cells. PMID:28522964

  2. Variability in Beta-Adrenergic Receptor Population in Cultured Chicken Muscle Cells

    NASA Technical Reports Server (NTRS)

    Young, Ronald B; Bridge, Kristin Y.; Vaughn, Jeffrey R.

    1998-01-01

    Investigations into expression of the beta-adrenergic receptor (bAR) in chicken skeletal muscle cells in culture were initiated because several beta-adrenergic receptor agonists are known to increase skeletal muscle protein deposition in avian and mammalian species. During initial attempts to study the bAR population on the surface of chicken skeletal muscle cells, we observed a high degree of variability that was later found to be the result of using different batches of horse serum in the cell culture media. The separation between total binding and nonspecific binding in cells grown in two serum samples was approximately two-fold The number of nuclei within multinucleated myotubes was not significantly different in cells grown in the two serum samples. To investigate whether these two sera had an effect on coupling efficiency between bAR population and cAMP production, the ability of these cells to synthesize cAMP was also assessed. Despite the two-fold difference in receptor population, the ability of these cells to synthesize cAMP was not significantly different. Because of the possible link between bAR population and muscle protein, we also determined if the quantity of the major skeletal muscle protein, myosin, was affected by conditions that so drastically affected the bAR population. The quantity of myosin heavy chain was not significantly different.

  3. Aging and differentiation in yeast populations: elders with different properties and functions.

    PubMed

    Palková, Zdena; Wilkinson, Derek; Váchová, Libuše

    2014-02-01

    Over the past decade, it has become evident that similarly to cells forming metazoan tissues, yeast cells have the ability to differentiate and form specialized cell types. Examples of yeast cellular differentiation have been identified both in yeast liquid cultures and within multicellular structures occupying solid surfaces. Most current knowledge on different cell types comes from studies of the spatiotemporal internal architecture of colonies developing on various media. With a few exceptions, yeast cell differentiation often concerns nongrowing, stationary-phase cells and leads to the formation of cell subpopulations differing in stress resistance, cell metabolism, respiration, ROS production, and others. These differences can affect longevity of particular subpopulations. In contrast to liquid cultures, where various cell types are dispersed within stationary-phase populations, cellular differentiation depends on the specific position of particular cells within multicellular colonies. Differentiated colonies, thus, resemble primitive multicellular organisms, in which the gradients of certain compounds and the position of cells within the structure affect cellular differentiation. In this review, we summarize and compare the properties of diverse types of differentiated chronologically aging yeast cells that have been identified in colonies growing on different media, as well as of those found in liquid cultures. © 2013 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.

  4. Ideal affect in daily life: implications for affective experience, health, and social behavior.

    PubMed

    Tsai, Jeanne L

    2017-10-01

    Over the last decade, researchers have increasingly demonstrated that ideal affect-the affective states that people value and ideally want to feel-shapes different aspects of daily life. Here I briefly review Affect Valuation Theory (AVT), which integrates ideal affect into existing models of affect and emotion by identifying the causes and consequences of variation in ideal affect. I then describe recent research that applies AVT to the valuation of negative states as well as more complex states, examines how ideal affect shapes momentary affective experience, suggests that ideal affect has both direct and indirect effects on health, and illustrates that people's ideal affect shapes how they judge and respond to others. Finally, I discuss the implications of cultural and individual differences in ideal affect for clinical, educational, work, and leisure settings. Copyright © 2017 Elsevier Ltd. All rights reserved.

  5. Influence of culture medium composition on relative mRNA abundances in domestic cat embryos.

    PubMed

    Hribal, R; Jewgenow, K; Braun, B C; Comizzoli, P

    2013-04-01

    Different culture conditions have been used to produce domestic cat embryos. As part of the in vitro procedures, the medium composition significantly affects the quality of the embryo development also. Quality assessments based on cleavage kinetics and blastomere symmetry are useful, but embryos also can differ in their relative gene expression patterns despite similar morphological characteristics. The aim of this study was to compare cat embryos produced with two different in vitro culture systems routinely used in two different laboratories [Smithsonian Conservation Biology Institute, Washington D.C., USA (SCBI) and Leibniz Institute for Zoo and Wildlife Research, Berlin, Germany (IZW)]. Specifically, relative mRNA expression patterns of critical genes for pre-implantation embryo development were assessed in both conditions. Embryos were produced in parallel in both culture systems by IVF using frozen-thawed ejaculated semen in the United States and fresh epididymal sperm in Germany. Success of embryo development in vitro was recorded as well as relative mRNA abundances [DNA methyltransferases 1 and 3A (DNMT1, DNMT3A), gap junction protein alpha 1 (GJA1), octamer-binding transcription factor 4 [OCT4], insulin-like growth factors 1 and 2 receptors (IGF1R, IGF2R), beta-actin (ACTB)] in pools of days 4-5 morulae by semi-quantitative RT-PCR assay. Percentages of cleaved embryos were similar (p > 0.05) between both culture systems, regardless of the location. OCT4 mRNA abundance was higher (p < 0.05) in embryos derived in the SCBI culture system compared with those from the IZW system when epididymal sperm was used for IVF. No clear correlation between the expression pattern and the culture system could be found for all other genes. It is suggested that OCT4 expression might be affected by the media composition in some conditions and can be the indicator of a better embryo quality. © 2012 Blackwell Verlag GmbH.

  6. Culture temperature affects redifferentiation and cartilaginous extracellular matrix formation in dedifferentiated human chondrocytes.

    PubMed

    Ito, Akira; Aoyama, Tomoki; Iijima, Hirotaka; Tajino, Junichi; Nagai, Momoko; Yamaguchi, Shoki; Zhang, Xiangkai; Kuroki, Hiroshi

    2015-05-01

    To date, there have been few studies on how temperature affects the phenotype and metabolism of human chondrocytes. Thus, the purpose of this study was to elucidate the effects of culture temperature on chondrocyte redifferentiation and extracellular matrix (ECM) formation using dedifferentiated mature human chondrocytes in vitro. Dedifferentiated chondrocytes were cultured in a pellet culture system for up to 21 days. The pellets were randomly divided into three groups with different culture temperature (32, 37, and 41°C). Chondrocyte redifferentiation and ECM formation were evaluated by wet weight, messenger ribonucleic acid (mRNA), histological, and biochemical analyses. The results showed that the wet weight and the mRNA expressions of collagen type II A1 and cartilage oligomeric matrix protein at 37°C were higher than the corresponding values at 32°C. The histological and biochemical analyses revealed that the syntheses of type II collagen and proteoglycan were promoted at 37°C compared to those at 32°C, whereas they were considerably inhibited at 41°C. In conclusion, the results obtained herein indicated that temperature affects chondrocyte redifferentiation and ECM formation, and modulation of temperature might thus represent an advantageous means to regulate the phenotype and biosynthetic activity of chondrocytes. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.

  7. Cultural differences in functional status measurement: analyses of person fit according to the Rasch model.

    PubMed

    Custers, J W; Hoijtink, H; van der Net, J; Helders, P J

    2000-01-01

    For many reasons it is preferable to use established health related outcome instruments. The validity of an instrument, however, can be affected when used in another culture or language other than what it was originally developed. In this paper, the outcome on functional status measurement using a preliminary version of the Dutch translated 'Pediatric Evaluation of Disability Inventory' (PEDI) was studied involving a sample of 20 non-disabled Dutch children and American peers, to see if a cross-cultural validation procedure is needed before using the instrument in the Netherlands. The Rasch model was used to analyse the Dutch data. Score profiles were not found to be compatible with the score profiles of American children. In particular, ten items were scored differently with strong indications that these were based on inter-cultural differences. Based on our study, it is argued that cross-cultural validation of the PEDI is necessary before using the instrument in the Netherlands.

  8. I feel your voice. Cultural differences in the multisensory perception of emotion.

    PubMed

    Tanaka, Akihiro; Koizumi, Ai; Imai, Hisato; Hiramatsu, Saori; Hiramoto, Eriko; de Gelder, Beatrice

    2010-09-01

    Cultural differences in emotion perception have been reported mainly for facial expressions and to a lesser extent for vocal expressions. However, the way in which the perceiver combines auditory and visual cues may itself be subject to cultural variability. Our study investigated cultural differences between Japanese and Dutch participants in the multisensory perception of emotion. A face and a voice, expressing either congruent or incongruent emotions, were presented on each trial. Participants were instructed to judge the emotion expressed in one of the two sources. The effect of to-be-ignored voice information on facial judgments was larger in Japanese than in Dutch participants, whereas the effect of to-be-ignored face information on vocal judgments was smaller in Japanese than in Dutch participants. This result indicates that Japanese people are more attuned than Dutch people to vocal processing in the multisensory perception of emotion. Our findings provide the first evidence that multisensory integration of affective information is modulated by perceivers' cultural background.

  9. Host-Specificity and Dynamics in Bacterial Communities Associated with Bloom-Forming Freshwater Phytoplankton

    PubMed Central

    Bagatini, Inessa Lacativa; Eiler, Alexander; Bertilsson, Stefan; Klaveness, Dag; Tessarolli, Letícia Piton; Vieira, Armando Augusto Henriques

    2014-01-01

    Many freshwater phytoplankton species have the potential to form transient nuisance blooms that affect water quality and other aquatic biota. Heterotrophic bacteria can influence such blooms via nutrient regeneration but also via antagonism and other biotic interactions. We studied the composition of bacterial communities associated with three bloom-forming freshwater phytoplankton species, the diatom Aulacoseira granulata and the cyanobacteria Microcystis aeruginosa and Cylindrospermopsis raciborskii. Experimental cultures incubated with and without lake bacteria were sampled in three different growth phases and bacterial community composition was assessed by 454-Pyrosequencing of 16S rRNA gene amplicons. Betaproteobacteria were dominant in all cultures inoculated with lake bacteria, but decreased during the experiment. In contrast, Alphaproteobacteria, which made up the second most abundant class of bacteria, increased overall during the course of the experiment. Other bacterial classes responded in contrasting ways to the experimental incubations causing significantly different bacterial communities to develop in response to host phytoplankton species, growth phase and between attached and free-living fractions. Differences in bacterial community composition between cyanobacteria and diatom cultures were greater than between the two cyanobacteria. Despite the significance, major differences between phytoplankton cultures were in the proportion of the OTUs rather than in the absence or presence of specific taxa. Different phytoplankton species favoring different bacterial communities may have important consequences for the fate of organic matter in systems where these bloom forming species occur. The dynamics and development of transient blooms may also be affected as bacterial communities seem to influence phytoplankton species growth in contrasting ways. PMID:24465807

  10. [Review on the association between social culture and HIV prevalence among MSM in China].

    PubMed

    He, Huijing; Lyu, Fan

    2015-11-01

    Social culture have significant influence on HIV transmission. Men who have sex with men (MSM), have their own sub-culture, by which made them very different with other population in social norm, behavior mode, as well as some HIV related behavior and the disease transmission risk. Up to date, study focused on the association between social culture and HIV transmission among MSM was limited, thus we reviewed relevant studies in the view of social culture and sub-culture of MSM, analyzed how social culture could potentially influence HIV transmission, and how sub-culture could affect MSM's friend-making, HIV risk behaviors in three aspects including social identity, social norm and social development. Furthermore, we provided some recommendations and suggestions on the future work.

  11. “Gambling disorder in older adults: a cross-cultural perspective”

    PubMed Central

    Medeiros, Gustavo Costa; Leppink, Eric; Yaemi, Ana; Mariani, Mirella; Tavares, Hermano; Grant, Jon

    2015-01-01

    INTRODUCTION Gambling Disorder (GD) in older adults is significantly increasing and became an important public health issue in different countries. However, little is known regarding GD in older adults. The prevalence and acceptance of gambling varies among different cultures and this raises the question of how and to what extent culture affects older gamblers. The majority of the important studies regarding GD in older adults have been conducted mainly in Anglo-Saxon cultures and little information is available regarding GD in other cultures. The objective of this paper is to perform the first standardized cross-cultural comparison regarding older adults presenting GD. METHODS The total studied sample involved 170 subjects: 89 from the Brazilian (BR) sample and 81 from the American (US) sample. It consisted of 67 men and 103, women (average age = 64.42, standard deviation = ±3,86). They were evaluated for socio-demographics, gambling behavior variables and psychiatric antecedents. RESULTS Overall, there were significant differences between BR and US older adults gamblers in marital status, onset of gambling activity, onset of GD and urge scores. DISCUSSION This study showed that there are important differences in gambling course, gambling behavior and personal antecedents between two samples of older adults presenting GD from countries with different social-cultural background. It weakens the possibility of generalization of results found in Anglo-Saxon countries to other cultures and reinforces for the need for development of research on GD in older adults outside the Anglo-Saxon culture. PMID:25612901

  12. Gambling disorder in older adults: a cross-cultural perspective.

    PubMed

    Medeiros, Gustavo Costa; Leppink, Eric; Yaemi, Ana; Mariani, Mirella; Tavares, Hermano; Grant, Jon

    2015-04-01

    Gambling disorder (GD) in older adults is significantly increasing and became an important public health issue in different countries. However, little is known regarding GD in older adults. The prevalence and acceptance of gambling vary among different cultures and this raises the question of how and to what extent culture affects older gamblers. The majority of the important studies regarding GD in older adults have been conducted mainly in Anglo-Saxon cultures and little information is available regarding GD in other cultures. The objective of this paper is to perform the first standardized cross-cultural comparison regarding older adults presenting GD. The total studied sample involved 170 subjects: 89 from the Brazilian (BR) sample and 81 from the American (US) sample. It consisted of 67 men and 103 women (average age=64.42, standard deviation=±3.86). They were evaluated for socio-demographics, gambling behavior variables and psychiatric antecedents. Overall, there were significant differences between BR and US older adult gamblers in marital status, onset of gambling activity, onset of GD and urge scores. This study showed that there are important differences in gambling course, gambling behavior and personal antecedents between two samples of older adults presenting GD from countries with different social-cultural background. It weakens the possibility of generalization of results found in Anglo-Saxon countries to other cultures and reinforces for the need for development of research on GD in older adults outside the Anglo-Saxon culture. Copyright © 2014 Elsevier Inc. All rights reserved.

  13. Black-White Differences in Achievement: The Importance of Wealth.

    ERIC Educational Resources Information Center

    Orr, Amy J.

    2003-01-01

    Argues that wealth can affect academic achievement, as well as help to explain the gap in black-white test scores and the racial achievement gap. Reveals that wealth affects achievement through its effect on the amount of cultural capital to which a child is exposed. Implications of the findings are discussed. (CAJ)

  14. "It's a Dog's Life": Culture, Empathy, Gender, and Domestic Violence Predict Animal Abuse in Adolescents-Implications for Societal Health.

    PubMed

    Plant, Malcolm; van Schaik, Paul; Gullone, Eleonora; Flynn, Clifton

    2016-07-01

    Whereas the majority of previous research conducted on animal abuse has been in environments where animal abuse is rarely evidenced, the current study investigated the ramifications of animal abuse in an environment wherein the national culture creates an ethos of the "social acceptability" of animal abuse in society. Two survey studies were conducted with adolescent participants, to investigate the role played by several factors in the prediction of animal abuse in this age group. In Study 1, with samples from two different national cultures (101 from Germany and 169 from Romania; 143 boys/135 girls; age 13 to 17), animal abuse was negatively associated with affective empathy and national culture; more frequent animal abuse was found in Romania. Affective empathy fully mediated the association between gender and animal abuse. Specifically, girls were found to be higher in affective empathy; in turn, participants who were higher in affective empathy committed less animal abuse. Witnessing animal abuse was also predictive of engaging in animal abuse, but not independent of national culture. In Study 2, 15-year-old males ( n = 21) and females ( n = 39) took part, 29 from rural and 31 from urban locations in Romania. Rural adolescents were more likely to abuse animals and had higher exposure to domestic violence, which (in turn) was associated with more animal abuse. The implications of these findings in a society where animal abuse is encouraged and enacted on a national scale are discussed.

  15. Universities as a Meeting Point with New Academic Knowledge, Society and Culture: Cognitive and Emotional Transitions during Higher Education

    ERIC Educational Resources Information Center

    Weiner-Levy, Naomi

    2008-01-01

    This paper examines how exposure to a different culture, acquired knowledge and everyday practices at Israeli universities affected the identities of the "First Women" students from the Druze minority who broke gender roles and turned to acquire higher education. The short distance between the Druze villages and the university required a…

  16. Students' Perceptions of Teacher Impact on Their Self-Directed Language Learning with Technology beyond the Classroom: Cases of Hong Kong and U.S.

    ERIC Educational Resources Information Center

    Lai, Chun; Li, Xiaoshi; Wang, Qiu

    2017-01-01

    Teachers are important social agents who affect students' cognitive and social behaviors, including students' self-directed use of technology for language learning outside the classroom. However, how teachers influence student behaviors may vary across cultures, and understanding how teacher influences vary across different cultures is critical to…

  17. Catholic School Leadership: School Climate and Culture and the Influence on Principal Satisfaction

    ERIC Educational Resources Information Center

    Beckley, Gary M.

    2012-01-01

    Today's Catholic educators face a very different world, creating a change in the role and responsibility of the principal. The purpose of this study was to determine to what extent the culture and climate affect satisfaction in the principal's role that warrants becoming and remaining a principal in today's Catholic schools. This…

  18. Cross-Cultural Examination of Depression Expression and Help-Seeking Behavior: A Comparative Study of American and Korean College Students.

    ERIC Educational Resources Information Center

    Yoo, Sung-Kyung; Skovholt, Thomas M.

    2001-01-01

    Examines cross-cultural differences in depression expression and help-seeking behavior among college students in the United States and Korea. Results indicate that the Korean students showed more somatization tendency, negative affect, and negative help-seeking behavior. Negative help-seeking behavior of Korean students was shown to relate to…

  19. Economic, Social, and Cultural Determinants of Life Satisfaction: Are There Differences between Asia and Europe?

    ERIC Educational Resources Information Center

    Jagodzinski, Wolfgang

    2010-01-01

    This paper investigates the influence of the economic, social, and cultural variables on life satisfaction in Asia and Europe. The second section sets a unifying theoretical framework for all three domains by defining life satisfaction as a function of aspirations and expectations which in turn are affected by micro- and macro-level variables. On…

  20. The sources of political orientations in post-industrial society: social class and education revisited.

    PubMed

    Van de Werfhorst, Herman G; de Graaf, Nan Dirk

    2004-06-01

    This paper studies the impact of social class and education on political orientation. We distinguish the 'old' middle class from a new class of social/cultural specialists. However, the difference in their political orientation may especially be related to the level and field of education; the new middle class is more highly educated and often in fields of study that extensively address social competencies, characteristics independently affecting political outcomes. Analyses on Dutch data showed that education is more important in the prediction of 'cultural' liberal issues than social class. Economically-oriented issues are more strongly affected by social class. This means that interests of the new middle class are served by liberal standpoints relating to a strong government and income redistribution policies, but not relating to cultural issues.

  1. Innovation in the collective brain

    PubMed Central

    Muthukrishna, Michael; Henrich, Joseph

    2016-01-01

    Innovation is often assumed to be the work of a talented few, whose products are passed on to the masses. Here, we argue that innovations are instead an emergent property of our species' cultural learning abilities, applied within our societies and social networks. Our societies and social networks act as collective brains. We outline how many human brains, which evolved primarily for the acquisition of culture, together beget a collective brain. Within these collective brains, the three main sources of innovation are serendipity, recombination and incremental improvement. We argue that rates of innovation are heavily influenced by (i) sociality, (ii) transmission fidelity, and (iii) cultural variance. We discuss some of the forces that affect these factors. These factors can also shape each other. For example, we provide preliminary evidence that transmission efficiency is affected by sociality—languages with more speakers are more efficient. We argue that collective brains can make each of their constituent cultural brains more innovative. This perspective sheds light on traits, such as IQ, that have been implicated in innovation. A collective brain perspective can help us understand otherwise puzzling findings in the IQ literature, including group differences, heritability differences and the dramatic increase in IQ test scores over time. PMID:26926282

  2. Innovation in the collective brain.

    PubMed

    Muthukrishna, Michael; Henrich, Joseph

    2016-03-19

    Innovation is often assumed to be the work of a talented few, whose products are passed on to the masses. Here, we argue that innovations are instead an emergent property of our species' cultural learning abilities, applied within our societies and social networks. Our societies and social networks act as collective brains. We outline how many human brains, which evolved primarily for the acquisition of culture, together beget a collective brain. Within these collective brains, the three main sources of innovation are serendipity, recombination and incremental improvement. We argue that rates of innovation are heavily influenced by (i) sociality, (ii) transmission fidelity, and (iii) cultural variance. We discuss some of the forces that affect these factors. These factors can also shape each other. For example, we provide preliminary evidence that transmission efficiency is affected by sociality--languages with more speakers are more efficient. We argue that collective brains can make each of their constituent cultural brains more innovative. This perspective sheds light on traits, such as IQ, that have been implicated in innovation. A collective brain perspective can help us understand otherwise puzzling findings in the IQ literature, including group differences, heritability differences and the dramatic increase in IQ test scores over time. © 2016 The Author(s).

  3. Environmental, policy, and cultural factors related to physical activity among rural, African American women.

    PubMed

    Sanderson, Bonnie; Littleton, MaryAnn; Pulley, LeaVonne

    2002-01-01

    Sixty-one African American women (ages 20-50 years) from a rural community in Alabama participated in six focus groups. Barriers to and enablers of physical activity were identified and grouped into personal, environmental (social and physical), policy, and cultural themes for qualitative analyses. Personal factors included motivation, perceived health, feeling tired, and lack of time; social environmental factors included support from friends, family, and issues related to child care; physical environmental factors included weather, access to facilities, availability of sidewalks or other places to walk; policy factors included personal safety concerns (loose dogs, traffic, etc.) and inflexible work environments. Some, but not all, women perceived cultural differences as a factor affecting physical activity levels. Differences in socioeconomic levels and time demands among women of different cultures were identified as factors that may influence physical activity. Participants provided suggestions for community-based physical activity interventions using an environmental approach.

  4. The Identification of Factors Affecting the Development and Practice of School-Based Counseling in Different National Contexts: A Grounded Theory Study Using a Worldwide Sample of Descriptive Journal Articles and Book Chapters

    ERIC Educational Resources Information Center

    Martin, Ian; Lauterbach, Alexandra; Carey, John

    2015-01-01

    A grounded theory methodology was used to analyze articles and book chapters describing the development and practice of school-based counseling in 25 different countries in order to identify the factors that affect development and practice. An 11-factor analytic framework was developed. Factors include: Cultural Factors, National Needs, Larger…

  5. The gender gap in sport performance: equity influences equality.

    PubMed

    Capranica, Laura; Piacentini, Maria Francesca; Halson, Shona; Myburgh, Kathryn H; Ogasawara, Etsuko; Millard-Stafford, Mindy

    2013-01-01

    Sport is recognized as playing a relevant societal role to promote education, health, intercultural dialogue, and the individual development, regardless of an individual's gender, race, age, ability, religion, political affiliation, sexual orientation, and socioeconomic background. Yet, it was not until the 2012 Summer Olympic Games in London that every country's delegation included a female competitor. The gender gap in sport, although closing, remains, due to biological differences affecting performance, but it is also influenced by reduced opportunity and sociopolitical factors that influence full female participation across a range of sports around the world. Until the cultural environment is equitable, scientific discussion related to physiological differences using methods that examine progression in male and female world-record performances is limited. This commentary is intended to provide a forum to discuss issues underlying gender differences in sport performance from a global perspective and acknowledge the influence of cultural and sociopolitical factors that continue to ultimately affect female performance.

  6. Cross-cultural validity of Morningness-Eveningness Stability Scale improved (MESSi) in Iran, Spain and Germany.

    PubMed

    Rahafar, Arash; Randler, Christoph; Díaz-Morales, Juan F; Kasaeian, Ali; Heidari, Zeinab

    2017-01-01

    Morningness-Eveningness Stability Scale improved (MESSi) is a newly constructed measure to assess circadian types and amplitude. In this study, we applied this measure to participants from three different countries: Germany, Spain and Iran. Confirmatory factorial analysis (CFA) of MESSi displayed mediocre fit in the three countries. Comparing increasingly stringent models using multigroup confirmatory factor analyses indicated at least partial measurement invariance (metric invariance) by country for Morning Affect and Distinctness subscales. Age was positively related to Morning Affect (MA), and negatively related to Eveningness (EV) and Distinctness (DI). Men reported higher MA than women, whereas women reported higher DI than men. Regarding country effect, Iranian participants reported highest MA compared to Spaniards and Germans, whereas Germans reported higher DI compared to Iranians and Spaniards. As a conclusion, our study corroborated the validity and reliability of MESSi across three different countries with different geographical and cultural characteristics.

  7. Cultural neuroeconomics of intertemporal choice.

    PubMed

    Takahashi, Taiki; Hadzibeganovic, Tarik; Cannas, Sergio A; Makino, Takaki; Fukui, Hiroki; Kitayama, Shinobu

    2009-01-01

    According to theories of cultural neuroscience, Westerners and Easterners may have distinct styles of cognition (e.g., different allocation of attention). Previous research has shown that Westerners and Easterners tend to utilize analytical and holistic cognitive styles, respectively. On the other hand, little is known regarding the cultural differences in neuroeconomic behavior. For instance, economic decisions may be affected by cultural differences in neurocomputational processing underlying attention; however, this area of neuroeconomics has been largely understudied. In the present paper, we attempt to bridge this gap by considering the links between the theory of cultural neuroscience and neuroeconomic theory of the role of attention in intertemporal choice. We predict that (i) Westerners are more impulsive and inconsistent in intertemporal choice in comparison to Easterners, and (ii) Westerners more steeply discount delayed monetary losses than Easterners. We examine these predictions by utilizing a novel temporal discounting model based on Tsallis' statistics (i.e. a q-exponential model). Our preliminary analysis of temporal discounting of gains and losses by Americans and Japanese confirmed the predictions from the cultural neuroeconomic theory. Future study directions, employing computational modeling via neural networks, are outlined and discussed.

  8. Gene-culture coevolution in whales and dolphins.

    PubMed

    Whitehead, Hal

    2017-07-24

    Whales and dolphins (Cetacea) have excellent social learning skills as well as a long and strong mother-calf bond. These features produce stable cultures, and, in some species, sympatric groups with different cultures. There is evidence and speculation that this cultural transmission of behavior has affected gene distributions. Culture seems to have driven killer whales into distinct ecotypes, which may be incipient species or subspecies. There are ecotype-specific signals of selection in functional genes that correspond to cultural foraging behavior and habitat use by the different ecotypes. The five species of whale with matrilineal social systems have remarkably low diversity of mtDNA. Cultural hitchhiking, the transmission of functionally neutral genes in parallel with selective cultural traits, is a plausible hypothesis for this low diversity, especially in sperm whales. In killer whales the ecotype divisions, together with founding bottlenecks, selection, and cultural hitchhiking, likely explain the low mtDNA diversity. Several cetacean species show habitat-specific distributions of mtDNA haplotypes, probably the result of mother-offspring cultural transmission of migration routes or destinations. In bottlenose dolphins, remarkable small-scale differences in haplotype distribution result from maternal cultural transmission of foraging methods, and large-scale redistributions of sperm whale cultural clans in the Pacific have likely changed mitochondrial genetic geography. With the acceleration of genomics new results should come fast, but understanding gene-culture coevolution will be hampered by the measured pace of research on the socio-cultural side of cetacean biology.

  9. Gene–culture coevolution in whales and dolphins

    PubMed Central

    Whitehead, Hal

    2017-01-01

    Whales and dolphins (Cetacea) have excellent social learning skills as well as a long and strong mother–calf bond. These features produce stable cultures, and, in some species, sympatric groups with different cultures. There is evidence and speculation that this cultural transmission of behavior has affected gene distributions. Culture seems to have driven killer whales into distinct ecotypes, which may be incipient species or subspecies. There are ecotype-specific signals of selection in functional genes that correspond to cultural foraging behavior and habitat use by the different ecotypes. The five species of whale with matrilineal social systems have remarkably low diversity of mtDNA. Cultural hitchhiking, the transmission of functionally neutral genes in parallel with selective cultural traits, is a plausible hypothesis for this low diversity, especially in sperm whales. In killer whales the ecotype divisions, together with founding bottlenecks, selection, and cultural hitchhiking, likely explain the low mtDNA diversity. Several cetacean species show habitat-specific distributions of mtDNA haplotypes, probably the result of mother–offspring cultural transmission of migration routes or destinations. In bottlenose dolphins, remarkable small-scale differences in haplotype distribution result from maternal cultural transmission of foraging methods, and large-scale redistributions of sperm whale cultural clans in the Pacific have likely changed mitochondrial genetic geography. With the acceleration of genomics new results should come fast, but understanding gene–culture coevolution will be hampered by the measured pace of research on the socio-cultural side of cetacean biology. PMID:28739936

  10. Cross-cultural differences in children's choices, categorizations, and evaluations of truths and lies.

    PubMed

    Fu, Genyue; Xu, Fen; Cameron, Catherine Ann; Leyman, Gail; Lee, Kang

    2007-03-01

    This study examined cross-cultural differences and similarities in children's moral understanding of individual- or collective-oriented lies and truths. Seven-, 9-, and 11-year-old Canadian and Chinese children were read stories about story characters facing moral dilemmas about whether to lie or tell the truth to help a group but harm an individual or vice versa. Participants chose to lie or to tell the truth as if they were the character (Experiments 1 and 2) and categorized and evaluated the story characters' truthful and untruthful statements (Experiments 3 and 4). Most children in both cultures labeled lies as lies and truths as truths. The major cultural differences lay in choices and moral evaluations. Chinese children chose lying to help a collective but harm an individual, and they rated it less negatively than lying with opposite consequences. Chinese children rated truth telling to help an individual but harm a group less positively than the alternative. Canadian children did the opposite. These findings suggest that cross-cultural differences in emphasis on groups versus individuals affect children's choices and moral judgments about truth and deception.

  11. Cross-cultural differences in risk perceptions of disasters.

    PubMed

    Gierlach, Elaine; Belsher, Bradley E; Beutler, Larry E

    2010-10-01

    Public risk perceptions of mass disasters carry considerable influences, both psychologically and economically, despite their oft-times imprecise nature. Prior research has identified the presence of an optimistic bias that affects risk perception, but there is a dearth of literature examining how these perceptions differ among cultures-particularly with regard to mass disasters. The present study explores differences among Japanese, Argentinean, and North American mental health workers in their rates of the optimistic bias in risk perceptions as contrasted between natural disasters and terrorist events. The results indicate a significant difference among cultures in levels of perceived risk that do not correspond to actual exposure rates. Japanese groups had the highest risk perceptions for both types of hazards and North Americans and Argentineans had the lowest risk perceptions for terrorism. Additionally, participants across all cultures rated risk to self as lower than risk to others (optimistic bias) across all disaster types. These findings suggest that cultural factors may have a greater influence on risk perception than social exposure, and that the belief that one is more immune to disasters compared to others may be a cross-cultural phenomenon. © 2010 Society for Risk Analysis.

  12. Intergroup Differences and Their Impact on African American Teachers.

    ERIC Educational Resources Information Center

    Mabokela, Reitumetse Obakeng; Madsen, Jean A.

    2003-01-01

    Examined how intergroup differences within suburban schools affected African American teachers' experiences. Organizational culture strongly influenced how whites treated their minority counterparts. Because the majority established norms, minorities were expected to comply with uniform sets of rules and regulations. Intergroup conflict arose…

  13. Just How Bad Negative Affect is for Your Health Depends on Culture

    PubMed Central

    Curhan, Katherine B.; Sims, Tamara; Markus, Hazel Rose; Kitayama, Shinobu; Karasawa, Mayumi; Kawakami, Norito; Love, Gayle D.; Coe, Christopher L.; Miyamoto, Yuri; Ryff, Carol D.

    2014-01-01

    Pressman, Lopez and Gallagher (2013) conclude that across the globe negative emotions are bad for one’s health. Yet, just how bad negative emotions are for health depends on culture. In U.S. American contexts, negative feelings are construed as the individual’s responsibility and as harmful. In Japanese contexts, negative feelings are construed as rooted in relationships and as natural. Using six clinically-relevant measures and two representative samples, we tested the hypothesis that negative affect is more strongly associated with poor health in the U.S. (n = 1,741) than in Japan (n = 988). Negative affect more strongly predicted poor health in the U.S. than in Japan for multi-item assessments of physical health (chronic conditions, physical functioning) and mental health (psychological well-being, self-esteem). There were no differences for single-item health assessments (life satisfaction, global health). These findings underscore the need for further theoretically-driven investigations of how cultural construals shape the emotion-health link. PMID:25304884

  14. Maternofetal outcome of asymptomatic bacteriuria among pregnant women in a Nigerian Teaching Hospital

    PubMed Central

    Izuchukwu, Kenneth Ebele; Oranu, Emmanuel Okwudili; Bassey, Goddy; Orazulike, Ngozi Clare

    2017-01-01

    Introduction Asymptomatic bacteriuria has been reported to be associated with adverse pregnancy outcome. This study sought to determine the prevalence and complications of asymptomatic bacteriuria amongst parturient in the University of Port Harcourt Teaching Hospital (UPTH). Methods The study was a prospective cohort study involving 220 eligible antenatal attendees. Urine culture and sensitivity was conducted for each participant and the fetomaternal outcome between affected and unaffected women were compared and p value <0.05 was considered significant. Results Sixty-five of the participants had asymptomatic bacteriuria giving a prevalence of 29.5%. Twenty-three (35.4%) cultures yielded Klebsiella spp while Fifty-eight (89%) of the cultured organisms were sensitive to Nitrofurantoin. There was no statistical difference in the rate of prelabour rupture of membranes, preeclampsia, preterm delivery, birth asphyxia and low birth weight between affected and unaffected women. Conclusion Contrary to widely held view, there was no significant increase in adverse pregnancy outcome amongst affected women. PMID:28819490

  15. Does the Cultural Formulation Interview for the fifth revision of the diagnostic and statistical manual of mental disorders (DSM-5) affect medical communication? A qualitative exploratory study from the New York site.

    PubMed

    Aggarwal, Neil K; Desilva, Ravi; Nicasio, Andel V; Boiler, Marit; Lewis-Fernández, Roberto

    2015-01-01

    Cross-cultural mental health researchers often analyze patient explanatory models of illness to optimize service provision. The Cultural Formulation Interview (CFI) is a cross-cultural assessment tool released in May 2013 with DSM-5 to revise shortcomings from the DSM-IV Outline for Cultural Formulation (OCF). The CFI field trial took place in 6 countries, 14 sites, and with 321 patients to explore its feasibility, acceptability, and clinical utility with patients and clinicians. We sought to analyze if and how CFI feasibility, acceptability, and clinical utility were related to patient-clinician communication. We report data from the New York site which enrolled 7 clinicians and 32 patients in 32 patient-clinician dyads. We undertook a data analysis independent of the parent field trial by conducting content analyses of debriefing interviews with all participants (n = 64) based on codebooks derived from frameworks for medical communication and implementation outcomes. Three coders created codebooks, coded independently, established inter-rater coding reliability, and analyzed if the CFI affects medical communication with respect to feasibility, acceptability, and clinical utility. Despite racial, ethnical, cultural, and professional differences within our group of patients and clinicians, we found that promoting satisfaction through the interview, eliciting data, eliciting the patient's perspective, and perceiving data at multiple levels were common codes that explained how the CFI affected medical communication. We also found that all but two codes fell under the implementation outcome of clinical utility, two fell under acceptability, and none fell under feasibility. Our study offers new directions for research on how a cultural interview affects patient-clinician communication. Future research can analyze how the CFI and other cultural interviews impact medical communication in clinical settings with subsequent effects on outcomes such as medication adherence, appointment retention, and health condition.

  16. Does the Cultural Formulation Interview (CFI) for the Fifth Revision of the Diagnostic and Statistical Manual of Mental Disorders (DSM-5) affect medical communication? A qualitative exploratory study from the New York site

    PubMed Central

    Aggarwal, Neil K.; DeSilva, Ravi; Nicasio, Andel V.; Boiler, Marit; Lewis-Fernández, Roberto

    2014-01-01

    Objectives Cross-cultural mental health researchers often analyze patient explanatory models of illness to optimize service provision. The Cultural Formulation Interview (CFI) is a cross-cultural assessment tool released in May 2013 with DSM-5 to revise shortcomings from the DSM-IV Outline for Cultural Formulation (OCF). The CFI field trial took place in 6 countries, 14 sites, and with 321 patients to explore its feasibility, acceptability, and clinical utility with patients and clinicians. We sought to analyze if and how CFI feasibility, acceptability, and clinical utility were related to patient-clinician communication. Design We report data from the New York site which enrolled 7 clinicians and 32 patients in 32 patient-clinician dyads. We undertook a data analysis independent of the parent field trial by conducting content analyses of debriefing interviews with all participants (n=64) based on codebooks derived from frameworks for medical communication and implementation outcomes. Three coders created codebooks, coded independently, established inter-rater coding reliability, and analyzed if the CFI affects medical communication with respect to feasibility, acceptability, and clinical utility. Results Despite racial, ethnic, cultural, and professional differences within our group of patients and clinicians, we found that promoting satisfaction through the interview, eliciting data, eliciting the patient’s perspective, and perceiving data at multiple levels were common codes that explained how the CFI affected medical communication. We also found that all but 2 codes fell under the implementation outcome of clinical utility, 2 fell under acceptability, and none fell under feasibility. Conclusion Our study offers new directions for research on how a cultural interview affects patient-clinician communication. Future research can analyze how the CFI and other cultural interviews impact medical communication in clinical settings with subsequent effects on outcomes such as medication adherence, appointment retention, and health condition. PMID:25372242

  17. The culture of time in neuropsychological assessment: exploring the effects of culture-specific time attitudes on timed test performance in Russian and American samples.

    PubMed

    Agranovich, Anna V; Panter, A T; Puente, Antonio E; Touradji, Pegah

    2011-07-01

    Cultural differences in time attitudes and their effect on timed neuropsychological test performance were examined in matched non-clinical samples of 100 Russian and American adult volunteers using 8 tests that were previously reported to be relatively free of cultural bias: Color Trails Test (CTT); Ruff Figural Fluency Test (RFFT); Symbol Digit Modalities Test (SDMT); and Tower of London-Drexel Edition (ToL(Dx)). A measure of time attitudes, the Culture of Time Inventory (COTI-33) was used to assess time attitudes potentially affecting time-limited testing. Americans significantly outscored Russians on CTT, SDMT, and ToL(Dx) (p,.05) while differences in RFFT scores only approached statistical significance. Group differences also emerged in COTI-33 factor scores, which partially mediated differences in performance on CTT-1, SDMT, and ToL(Dx) initiation time, but did not account for the effect of culture on CTT-2. Significant effect of culture was revealed in ratings of familiarity with testing procedures that was negatively related to CTT, ToL(Dx), and SDMT scores. Current findings indicated that attitudes toward time may influence results of time limited testing and suggested that individuals who lack familiarity with timed testing procedures tend to obtain lower scores on timed tests.

  18. Game-Changing Innovations: How Culture Can Change the Parameters of Its Own Evolution and Induce Abrupt Cultural Shifts.

    PubMed

    Kolodny, Oren; Creanza, Nicole; Feldman, Marcus W

    2016-12-01

    One of the most puzzling features of the prehistoric record of hominid stone tools is its apparent punctuation: it consists of abrupt bursts of dramatic change that separate long periods of largely unchanging technology. Within each such period, small punctuated cultural modifications take place. Punctuation on multiple timescales and magnitudes is also found in cultural trajectories from historical times. To explain these sharp cultural bursts, researchers invoke such external factors as sudden environmental change, rapid cognitive or morphological change in the hominids that created the tools, or replacement of one species or population by another. Here we propose a dynamic model of cultural evolution that accommodates empirical observations: without invoking external factors, it gives rise to a pattern of rare, dramatic cultural bursts, interspersed by more frequent, smaller, punctuated cultural modifications. Our model includes interdependent innovation processes that occur at different rates. It also incorporates a realistic aspect of cultural evolution: cultural innovations, such as those that increase food availability or that affect cultural transmission, can change the parameters that affect cultural evolution, thereby altering the population's cultural dynamics and steady state. This steady state can be regarded as a cultural carrying capacity. These parameter-changing cultural innovations occur very rarely, but whenever one occurs, it triggers a dramatic shift towards a new cultural steady state. The smaller and more frequent punctuated cultural changes, on the other hand, are brought about by innovations that spur the invention of further, related, technology, and which occur regardless of whether the population is near its cultural steady state. Our model suggests that common interpretations of cultural shifts as evidence of biological change, for example the appearance of behaviorally modern humans, may be unwarranted.

  19. Game-Changing Innovations: How Culture Can Change the Parameters of Its Own Evolution and Induce Abrupt Cultural Shifts

    PubMed Central

    2016-01-01

    One of the most puzzling features of the prehistoric record of hominid stone tools is its apparent punctuation: it consists of abrupt bursts of dramatic change that separate long periods of largely unchanging technology. Within each such period, small punctuated cultural modifications take place. Punctuation on multiple timescales and magnitudes is also found in cultural trajectories from historical times. To explain these sharp cultural bursts, researchers invoke such external factors as sudden environmental change, rapid cognitive or morphological change in the hominids that created the tools, or replacement of one species or population by another. Here we propose a dynamic model of cultural evolution that accommodates empirical observations: without invoking external factors, it gives rise to a pattern of rare, dramatic cultural bursts, interspersed by more frequent, smaller, punctuated cultural modifications. Our model includes interdependent innovation processes that occur at different rates. It also incorporates a realistic aspect of cultural evolution: cultural innovations, such as those that increase food availability or that affect cultural transmission, can change the parameters that affect cultural evolution, thereby altering the population’s cultural dynamics and steady state. This steady state can be regarded as a cultural carrying capacity. These parameter-changing cultural innovations occur very rarely, but whenever one occurs, it triggers a dramatic shift towards a new cultural steady state. The smaller and more frequent punctuated cultural changes, on the other hand, are brought about by innovations that spur the invention of further, related, technology, and which occur regardless of whether the population is near its cultural steady state. Our model suggests that common interpretations of cultural shifts as evidence of biological change, for example the appearance of behaviorally modern humans, may be unwarranted. PMID:28036346

  20. The characteristics of patients with pulmonary Mycobacterium avium-intracellulare complex disease diagnosed by bronchial lavage culture compared to those diagnosed by sputum culture.

    PubMed

    Maekawa, Koichi; Naka, Megumi; Shuto, Saki; Harada, Yuka; Ikegami, Yumiko

    2017-09-01

    The utility of bronchoscopy for the diagnosis of pulmonary Mycobacterium avium-intracellulare complex (MAC) disease has been reported; however, which patients require bronchoscopy remains unclear. Our objective was to identify the characteristics of the patients in whom bronchoscopy is needed for the diagnosis of MAC disease. Fifty-four patients with pulmonary MAC disease were divided into two groups according to established diagnostic criteria: 39 patients were diagnosed by sputum culture and 15 patients were diagnosed by bronchial lavage culture. We analysed the differences in demographic and clinical characteristics as well as microbiological and radiological data between the two groups. There were no significant differences in age, sex, smoking status, MAC species, underlying diseases, or steroid use. Significantly more patients diagnosed by sputum culture than bronchial lavage culture had a positive sputum smear for acid-fast bacilli (79.5% vs. 0.0%, respectively; p < 0.001) and any symptoms (75.3% vs. 46.2%, respectively; p = 0.0059). No significant differences were found in the prevalence of each computed tomography finding, including nodules, air-space disease, bronchiectasis, and cavities. However, more patients diagnosed by sputum culture than bronchial lavage culture had abnormalities in the left upper division (48.7% vs. 13.3%, respectively; p = 0.017) and higher numbers of affected lobes (4.3 ± 1.4 vs. 3.3 ± 1.6, respectively; p = 0.034). If patients suspected of having pulmonary MAC disease have a negative sputum smear, no symptoms, no abnormal findings in the left upper division, or fewer affected lobes on computed tomography, bronchoscopy might be needed for the diagnosis. Copyright © 2017 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

  1. Cultural understandings of resilience: roots for wings in the development of affective resources for resilience.

    PubMed

    Cameron, Catherine Ann; Ungar, Michael; Liebenberg, Linda

    2007-04-01

    The authors examine one of the precursors of positive development: attachment. Attachment and the positive growth it portends for populations of children under stress underpins positive developmental outcomes now termed "resilience." Resilience may be understood as a process of adaptation to adversity that is scaffolded by environmental, cultural, social, psychologic, and physiologic processes. The authors focus on two methodologically different approaches to studying attachments that contribute to resilient functioning at two different phases of development (toddlerhood and adolescence). The authors examine the cultural differences found in manifestations of resilience in different countries and cultures. Organized around this theme of attachment, the authors identify adaptive factors in resistance to risk from adverse circumstances. The authors strive to identify how the adaptation involved in attachment relations can protect against vulnerability. The authors conclude with a description of the processes that might help us to understand situational, experiential, and personal resources that intersect to protect the developing individual against assaults on normal growth and development.

  2. Cultural context moderates the relationship between emotion control values and cardiovascular challenge versus threat responses.

    PubMed

    Mauss, Iris B; Butler, Emily A

    2010-07-01

    Cultural context affects people's values regarding emotions, as well as their experiential and behavioral but not autonomic physiological responses to emotional situations. Little research, however, has examined how cultural context influences the relationships among values and emotional responding. Specifically, depending on their cultural context, individuals' values about emotion control (ECV; the extent to which they value emotion control) may have differing meanings, and as such, be associated with differing responses in emotional situations. We examined this possibility by testing the effect of two cultural contexts (28 female Asian-American (AA) versus 28 female European-American (EA) undergraduate students) on the associations between individuals' ECV and emotional responding (experiential, behavioral, and cardiovascular) to a relatively neutral film clip and a laboratory anger provocation. In the AA group, greater ECV were associated with reduced anger experience and behavior, and a challenge pattern of cardiovascular responding. In the EA group, greater ECV were associated with reduced anger behavior but not anger experience, and a threat pattern of cardiovascular responding. These results are consistent with the notion that individuals' values about emotion are associated with different meanings in different cultural contexts, and in turn, with different emotional and cardiovascular responses. Copyright © 2009 Elsevier B.V. All rights reserved.

  3. Cultural Issues in Disclosures of Child Sexual Abuse

    ERIC Educational Resources Information Center

    Fontes, Lisa Aronson; Plummer, Carol

    2010-01-01

    Cultural norms affect the likelihood that child sexual abuse will be discovered by an adult or disclosed by a child. Cultural norms also affect whether abused children's families will report child sexual abuse to authorities. This article explores the ways ethnic and religious culture affect child sexual abuse disclosure and reporting, both in the…

  4. Water relations in culture media influence maturation of avocado somatic embryos.

    PubMed

    Márquez-Martín, Belén; Sesmero, Rafael; Quesada, Miguel A; Pliego-Alfaro, Fernando; Sánchez-Romero, Carolina

    2011-11-15

    Application of transformation and other biotechnological tools in avocado (Persea americana Mill.) is hampered by difficulties in obtaining mature somatic embryos capable of germination at an acceptable rate. In this work, we evaluated the effect of different compounds affecting medium water relations on maturation of avocado somatic embryos. Culture media were characterized with respect to gel strength, water potential and osmotic potential. Improved production of mature somatic embryos was achieved with gelling agent concentrations higher than those considered standard. The osmotic agents such as sorbitol and PEG did not have positive effects on embryo maturation. The number of w-o mature somatic embryos per culture was positively correlated with medium gel strength. Gel strength was significantly affected by gelling agent type as well as by gelling agent and PEG concentration. Medium water potential was influenced by sorbitol concentration; incorporation of PEG to a culture medium did not affect medium water potential. The highest maturation results were achieved on a medium gelled with 10 gl(-1) agar. Moreover, these somatic embryos had improved germination rates. These results corroborate the role of water restriction as a key factor controlling maturation of somatic embryos. Copyright © 2011 Elsevier GmbH. All rights reserved.

  5. Physiological characterization of ocular melanosis-affected canine melanocytes.

    PubMed

    Dawson-Baglien, Ethan M; Noland, Erica L; Sledge, Dodd G; Kiupel, Matti; Petersen-Jones, Simon M

    2018-04-27

    Cairn terriers with ocular melanosis (OM) accumulate large, heavily pigmented melanocytes in the anterior uvea. Darkly pigmented plaques develop within the sclera, leading us to hypothesize that OM uveal melanocytes may have an abnormal migratory capacity. Globes from OM-affected Cairn terriers and unaffected control eyes enucleated for reasons unrelated to this study were used for immunohistochemistry and to culture melanocytes for in vitro cell behavior assays. The scleral plaques of six dogs were immunolabeled for HMB-45, MelanA, PNL2, CD18, CD204, and Iba-1 and compared with the pigment cells accumulated within the irides. Cultured uveal melanocytes from OM-affected and control dogs were compared using conventional assays measuring cell proliferation, invasion capability, and melanin production. Melanocytes isolated from OM eyes had significantly elevated levels of per-cell melanin content and production compared to controls. The majority of pigmented cells in the scleral plaques were HMB45 positive indicating a melanocytic origin. Many were also CD18 positive. No differences were observed between cultured melanocytes from OM-affected and control uvea for standard in vitro proliferation or invasion assays. Pigmented cells which accumulate in the sclera of OM-affected Cairn terriers are predominantly melanocytes; however, in vitro assays of uveal melanocytes did not reveal differences in migratory behavior between OM and control cells. Migratory behavior of OM-melanocytes may be environment-dependent. We suggest that RNA sequencing and differential expression analysis would be a useful next step in understanding this disease. © 2018 American College of Veterinary Ophthalmologists.

  6. Pathogen inactivation of human serum facilitates its clinical use for islet cell culture and subsequent transplantation.

    PubMed

    Ståhle, Magnus U; Brandhorst, Daniel; Korsgren, Olle; Knutson, Folke

    2011-01-01

    Serum is regarded as an essential supplement to promote survival and growth of cells during culture. However, the potential risk of transmitting diseases disqualifies the use of serum for clinical cell therapy in most countries. Hence, most clinical cell therapy programs have replaced human serum with human serum albumin, which can result in inferior quality of released cell products. Photochemical treatment of different blood products utilizing Intercept® technology has been shown to inactivate a broad variety of pathogens of RNA and DNA origin. The present study assesses the feasibility of using pathogen-inactivated, blood group-compatible serum for use in human pancreatic islet culture. Isolated human islets were cultured at 37°C for 3-4 days in CMRL 1066 supplemented with 10% of either pathogen-inactivated or nontreated human serum. Islet quality assessment included glucose-stimulated insulin release (perifusion), ADP/ATP ratio, cytokine expression, and posttransplant function in diabetic nude mice. No differences were found between islets cultured in pathogen-inactivated or control serum regarding stimulated insulin release, intracellular insulin content, and ADP/ATP ratio. Whether media was supplemented with treated or nontreated serum, islet expression of IL-6, IL-8, MCP-1, or tissue factor was not affected. The final diabetes-reversal rate of mice receiving islets cultured in pathogen-inactivated or nontreated serum was 78% and 87%, respectively (NS). As reported here, pathogen-inactivated human serum does not affect viability or functional integrity of cultured human islets. The implementation of this technology for RNA- and DNA-based pathogen inactivation should enable reintroduction of human serum for clinical cell therapy.

  7. Cross-Cultural Differences in the Neural Correlates of Specific and General Recognition

    PubMed Central

    Paige, Laura E.; Ksander, John C.; Johndro, Hunter A.; Gutchess, Angela H.

    2017-01-01

    Research suggests that culture influences how people perceive the world, which extends to memory specificity, or how much perceptual detail is remembered. The present study investigated cross-cultural differences (Americans vs. East Asians) at the time of encoding in the neural correlates of specific vs. general memory formation. Participants encoded photos of everyday items in the scanner and 48 hours later completed a surprise recognition test. The recognition test consisted of same (i.e., previously seen in scanner), similar (i.e., same name, different features), or new photos (i.e., items not previously seen in scanner). For Americans compared to East Asians, we predicted greater activation in the hippocampus and right fusiform for specific memory at recognition, as these regions were implicated previously in encoding perceptual details. Results revealed that East Asians activated the left fusiform and left hippocampus more than Americans for specific vs. general memory. Follow-up analyses ruled out alternative explanations of retrieval difficulty and familiarity for this pattern of cross-cultural differences at encoding. Results overall suggest that culture should be considered as another individual difference that affects memory specificity and modulates neural regions underlying these processes. PMID:28256199

  8. Cross-cultural differences in the neural correlates of specific and general recognition.

    PubMed

    Paige, Laura E; Ksander, John C; Johndro, Hunter A; Gutchess, Angela H

    2017-06-01

    Research suggests that culture influences how people perceive the world, which extends to memory specificity, or how much perceptual detail is remembered. The present study investigated cross-cultural differences (Americans vs East Asians) at the time of encoding in the neural correlates of specific versus general memory formation. Participants encoded photos of everyday items in the scanner and 48 h later completed a surprise recognition test. The recognition test consisted of same (i.e., previously seen in scanner), similar (i.e., same name, different features), or new photos (i.e., items not previously seen in scanner). For Americans compared to East Asians, we predicted greater activation in the hippocampus and right fusiform for specific memory at recognition, as these regions were implicated previously in encoding perceptual details. Results revealed that East Asians activated the left fusiform and left hippocampus more than Americans for specific versus general memory. Follow-up analyses ruled out alternative explanations of retrieval difficulty and familiarity for this pattern of cross-cultural differences at encoding. Results overall suggest that culture should be considered as another individual difference that affects memory specificity and modulates neural regions underlying these processes. Copyright © 2017 Elsevier Ltd. All rights reserved.

  9. Cultural Transmission on the Taskscape: Exploring the Effects of Taskscape Visibility on Cultural Diversity.

    PubMed

    Premo, L S; Tostevin, Gilbert B

    2016-01-01

    Culturally transmitted behavior can be structured in its performance both geographically and temporally, in terms of where and when implements are made and used on the landscape (what Ingold calls "the taskscape"). Yet cultural transmission theory has not yet explored the consequences of behaviors transmitted differently due to their enactment at different taskscape locations, what Tostevin calls "taskscape visibility." Here, we use computer simulations to explore how taskscape visibility and forager mobility affect the diversity of two selectively neutral culturally transmitted traits within a single population of social learners. The trait that can be transmitted from residential bases only (lower taskscape visibility) shows greater diversity than the trait that can be transmitted from residential bases and logistical camps (higher taskscape visibility). In addition, increased logistical mobility has a positive effect on the diversity of the trait with the lower taskscape visibility while it generally shows little to no effect on the diversity of the trait with higher taskscape visibility. Without an appreciation for the ways in which taskscape visibility and mobility can structure cultural transmission in space and through time, the difference in the observed equilibrium diversity levels of the two traits might be incorrectly interpreted as resulting from qualitatively different forms of biased cultural transmission. The results of our simulation experiment suggest that researchers may need to take the taskscape visibility into account when inferring cultural transmission from archaeological data.

  10. Cultural diversity, economic development and societal instability.

    PubMed

    Nettle, Daniel; Grace, James B; Choisy, Marc; Cornell, Howard V; Guégan, Jean-François; Hochberg, Michael E

    2007-09-26

    Social scientists have suggested that cultural diversity in a nation leads to societal instability. However, societal instability may be affected not only by within-nation or alpha diversity, but also diversity between a nation and its neighbours or beta diversity. It is also necessary to distinguish different domains of diversity, namely linguistic, ethnic and religious, and to distinguish between the direct effects of diversity on societal instability, and effects that are mediated by economic conditions. We assembled a large cross-national dataset with information on alpha and beta cultural diversity, economic conditions, and indices of societal instability. Structural equation modeling was used to evaluate the direct and indirect effects of cultural diversity on economics and societal stability. Results show that different types and domains of diversity have interacting effects. As previously documented, linguistic alpha diversity has a negative effect on economic performance, and we show that it is largely through this economic mechanism that it affects societal instability. For beta diversity, the higher the linguistic diversity among nations in a region, the less stable the nation. But, religious beta diversity has the opposite effect, reducing instability, particularly in the presence of high linguistic diversity. Within-nation linguistic diversity is associated with reduced economic performance, which, in turn, increases societal instability. Nations which differ linguistically from their neighbors are also less stable. However, religious diversity between neighboring nations has the opposite effect, decreasing societal instability.

  11. Cultural diversity, economic development and societal instability

    USGS Publications Warehouse

    Nettle, D.; Grace, J.B.; Choisy, M.; Cornell, H.V.; Guegan, J.-F.; Hochberg, M.E.

    2007-01-01

    Background. Social scientists have suggested that cultural diversity in a nation leads to societal instability. However, societal instability may be affected not only by within-nation on ?? diversity, but also diversity between a nation and its neighbours or ?? diversity. It is also necessary to distinguish different domains of diversity, namely linguistic, ethnic and religious, and to distinguish between the direct effects of diversity on societal instability, and effects that are mediated by economic conditions. Methodology/Principal Findings. We assembled a large cross-national dataset with information on ?? and ?? cultural diversity, economic conditions, and indices of societal instability. Structural equation modeling was used to evaluate the direct and indirect effects of cultural diversity on economics and societal stability. Results show that different type and domains of diversity have interacting effects. As previously documented, linguistic ?? diversity has a negative effect on economic performance, and we show that it is largely through this economic mechanism that it affects societal instability. For ?? diversity, the higher the linguistic diversity among nations in a region, the less stable the nation. But, religious ?? diversity has the opposite effect, reducing instability, particularly in the presence of high linguistic diversity. Conclusions. Within-nation linguistic diversity is associated with reduced economic performance, which, in turn, increases societal instability. Nations which differ linguistically from their neighbors are also less stable. However, religious diversity between, neighboring nations has the opposite effect, decreasing societal instability.

  12. Culture temperature affects human chondrocyte messenger RNA expression in monolayer and pellet culture systems.

    PubMed

    Ito, Akira; Nagai, Momoko; Tajino, Junichi; Yamaguchi, Shoki; Iijima, Hirotaka; Zhang, Xiangkai; Aoyama, Tomoki; Kuroki, Hiroshi

    2015-01-01

    Cell-based therapy has been explored for articular cartilage regeneration. Autologous chondrocyte implantation is a promising cell-based technique for repairing articular cartilage defects. However, there are several issues such as chondrocyte de-differentiation. While numerous studies have been designed to overcome some of these issues, only a few have focused on the thermal environment that can affect chondrocyte metabolism and phenotype. In this study, the effects of different culture temperatures on human chondrocyte metabolism- and phenotype-related gene expression were investigated in 2D and 3D environments. Human chondrocytes were cultured in a monolayer or in a pellet culture system at three different culture temperatures (32°C, 37°C, and 41°C) for 3 days. The results showed that the total RNA level, normalized to the threshold cycle value of internal reference genes, was higher at lower temperatures in both culture systems. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and citrate synthase (CS), which are involved in glycolysis and the citric acid cycle, respectively, were expressed at similar levels at 32°C and 37°C in pellet cultures, but the levels were significantly lower at 41°C. Expression of the chondrogenic markers, collagen type IIA1 (COL2A1) and aggrecan (ACAN), was higher at 37°C than at 32°C and 41°C in both culture systems. However, this phenomenon did not coincide with SRY (sex-determining region Y)-box 9 (SOX9), which is a fundamental transcription factor for chondrogenesis, indicating that a SOX9-independent pathway might be involved in this phenomenon. In conclusion, the expression of chondrocyte metabolism-related genes at 32°C was maintained or enhanced compared to that at 37°C. However, chondrogenesis-related genes were further induced at 37°C in both culture systems. Therefore, manipulating the culture temperature may be an advantageous approach for regulating human chondrocyte metabolic activity and chondrogenesis.

  13. Cross-cultural differences in beliefs and practices that affect the language spoken to children: mothers with Indian and Western heritage.

    PubMed

    Simmons, Noreen; Johnston, Judith

    2007-01-01

    Speech-language pathologists often advise families about interaction patterns that will facilitate language learning. This advice is typically based on research with North American families of European heritage and may not be culturally suited for non-Western families. The goal of the project was to identify differences in the beliefs and practices of Indian and Euro-Canadian mothers that would affect patterns of talk to children. A total of 47 Indian mothers and 51 Euro-Canadian mothers of preschool age children completed a written survey concerning child-rearing practices and beliefs, especially those about talk to children. Discriminant analyses indicated clear cross-cultural differences and produced functions that could predict group membership with a 96% accuracy rate. Items contributing most to these functions concerned the importance of family, perceptions of language learning, children's use of language in family and society, and interactions surrounding text. Speech-language pathologists who wish to adapt their services for families of Indian heritage should remember the centrality of the family, the likelihood that there will be less emphasis on early independence and achievement, and the preference for direct instruction.

  14. Causes, coping, and culture: a comparative survey study on representation of back pain in three Swiss language regions.

    PubMed

    Schulz, Peter J; Hartung, Uwe; Riva, Silvia

    2013-01-01

    This study intends to contribute to a research tradition that asks how causal attributions of illnesses affect coping behavior. Causal attributions are understood as the most important element of illness representations and coping as a means to preserve quality of life. The issue is applied to a condition so far often neglected in research on illness representations-back pain-and a third concept is added to the picture: culture. The aim of this study is (a) to explore the causal factors to which persons with back pain attribute the further course of their illness, (b) to find out whether the attributed causes are predictors of coping maxims, and (c) to find out whether cultural factors affect attributions and coping and moderate the relationship between the two. A total of 1259 gainfully employed or self-employed persons with recent episodes of back pain were recruited in the three language regions of Switzerland. They were asked to complete a structured online interview, measuring among many other variables attributed causes, coping maxims, and affiliation to one of the Swiss micro-cultures (German-, French- or Italian-speaking). Attributed causes of the illness that can be influenced by a patient go along with more active coping styles. Cultural affiliation impacts on coping maxims independently, but culture moderates the relationship of attributed causes and coping maxims only in two of twenty possible cases. The results show that cultural differences can be analytically incorporated in the models of illness representations. Results may help to improve healthcare providers' communication with patients and plan public health campaigns. The approach to micro-cultural differences and the substantive relationships between alterability of causes and activity in coping may help the further development of models of illness representations.

  15. Causes, Coping, and Culture: A Comparative Survey Study on Representation of Back Pain in Three Swiss Language Regions

    PubMed Central

    Schulz, Peter J.; Hartung, Uwe; Riva, Silvia

    2013-01-01

    Introduction This study intends to contribute to a research tradition that asks how causal attributions of illnesses affect coping behavior. Causal attributions are understood as the most important element of illness representations and coping as a means to preserve quality of life. The issue is applied to a condition so far often neglected in research on illness representations–back pain–and a third concept is added to the picture: culture. Aim The aim of this study is (a) to explore the causal factors to which persons with back pain attribute the further course of their illness, (b) to find out whether the attributed causes are predictors of coping maxims, and (c) to find out whether cultural factors affect attributions and coping and moderate the relationship between the two. Methods A total of 1259 gainfully employed or self-employed persons with recent episodes of back pain were recruited in the three language regions of Switzerland. They were asked to complete a structured online interview, measuring among many other variables attributed causes, coping maxims, and affiliation to one of the Swiss micro-cultures (German-, French- or Italian-speaking). Results Attributed causes of the illness that can be influenced by a patient go along with more active coping styles. Cultural affiliation impacts on coping maxims independently, but culture moderates the relationship of attributed causes and coping maxims only in two of twenty possible cases. Implications The results show that cultural differences can be analytically incorporated in the models of illness representations. Results may help to improve healthcare providers’ communication with patients and plan public health campaigns. The approach to micro-cultural differences and the substantive relationships between alterability of causes and activity in coping may help the further development of models of illness representations. PMID:24223756

  16. The Effect of Different Cultural Lenses on Reliability and Validity in Observational Data: The Example of Chinese Immigrant Parent-Toddler Dinner Interactions

    ERIC Educational Resources Information Center

    Wang, Yan Z.; Wiley, Angela R.; Zhou, Xiaobin

    2007-01-01

    This study used a mixed methodology to investigate reliability, validity, and analysis level with Chinese immigrant observational data. European-American and Chinese coders quantitatively rated 755 minutes of Chinese immigrant parent-toddler dinner interactions on parental sensitivity, intrusiveness, detachment, negative affect, positive affect,…

  17. Pronominal Differences in Languages of Sabah and Sarawak and the Effect on the Acquisition of Bahasa Malaysia.

    ERIC Educational Resources Information Center

    Lee, Eileen Yen Ee; Boutin, Michael E.

    Bilingual education programs must be based on a sound understanding of the linguistic, social, psychological, and cultural factors affecting students. This paper is concerned with linguistic factors that affect the acquisition of Bahasa Malaysia (BM) pronouns by non-native speakers from Sabah and Sarawak. Properties in the personal pronoun systems…

  18. Patient-centered care: the key to cultural competence.

    PubMed

    Epner, D E; Baile, W F

    2012-04-01

    Much of the early literature on 'cultural competence' focuses on the 'categorical' or 'multicultural' approach, in which providers learn relevant attitudes, values, beliefs, and behaviors of certain cultural groups. In essence, this involves learning key 'dos and don'ts' for each group. Literature and educational materials of this kind focus on broad ethnic, racial, religious, or national groups, such as 'African American', 'Hispanic', or 'Asian'. The problem with this categorical or 'list of traits' approach to clinical cultural competence is that culture is multidimensional and dynamic. Culture comprises multiple variables, affecting all aspects of experience. Cultural processes frequently differ within the same ethnic or social group because of differences in age cohort, gender, political association, class, religion, ethnicity, and even personality. Culture is therefore a very elusive and nebulous concept, like art. The multicultural approach to cultural competence results in stereotypical thinking rather than clinical competence. A newer, cross cultural approach to culturally competent clinical practice focuses on foundational communication skills, awareness of cross-cutting cultural and social issues, and health beliefs that are present in all cultures. We can think of these as universal human beliefs, needs, and traits. This patient centered approach relies on identifying and negotiating different styles of communication, decision-making preferences, roles of family, sexual and gender issues, and issues of mistrust, prejudice, and racism, among other factors. In the current paper, we describe 'cultural' challenges that arise in the care of four patients from disparate cultures, each of whom has advanced colon cancer that is no longer responding to chemotherapy. We then illustrate how to apply principles of patient centered care to these challenges.

  19. Are Asian cultures really less ageist than Western ones? It depends on the questions asked.

    PubMed

    Vauclair, Christin-Melanie; Hanke, Katja; Huang, Li-Li; Abrams, Dominic

    2017-04-01

    Ageism is an increasing concern in ageing populations such as Asia and Europe. A prevalent assumption in psychology is that Eastern cultures may be less prone to ageism because of norms and values that honour and respect elders. Yet, evidence for this culture hypothesis is inconclusive. The current study examines this issue by comparing attitudes towards older people in an Eastern and Western samples of 184 young people from the UK and 249 from Taiwan. Attitudes to old age were measured both as meta-perceptions (the perceived normative context) and personal attitudes in regard to the cognitive, affective and behavioural components of ageism. Consistent with the culture hypothesis, meta-perceptions about competence and admiration were more positive in Taiwan than in the UK, yet other meta-perceptions were more negative pointing to the existence of old age subtypes. Personal attitudes about older people in regard to the affective and behavioural, but not the cognitive component, were more negative in Taiwan than in the UK. Thus, cultural differences in ageism are more nuanced than suggested by previous research. The importance of distinguishing between the normative context and personal attitudes as well as the different components of ageism is highlighted by the present findings. © 2016 The Authors. International Journal of Psychology published by John Wiley & Sons Ltd on behalf of International Union of Psychological Science.

  20. Cell culture-adaptive mutations of NS5A affect replication of hepatitis C virus differentially depending on the viral genotypes.

    PubMed

    Chung, Aeri; Jin, Bora; Han, Kwang-Hyub; Ahn, Sang Hoon; Kim, Seungtaek

    2017-01-01

    Most of HCV RNAs require cell culture-adaptive mutations for efficient replication in cell culture and a number of such mutations have been described including a well-known S2204I substitution mutation in NS5A protein. In contrast, the replication of genotype 2a JFH1 RNA in cell culture does not require any cell culture-adaptive mutation. Rather, the presence of S2204I mutation impaired the JFH1 RNA replication. In this study, we examined the effect of reversions and substitutions of NS5A cell culture-adaptive mutations on virus replication in different genotypic backgrounds after either placing genotype 1a NS5A in the genotype 2a JFH1 or vice versa. The results from this investigation suggest that the S2204I mutation affects HCV RNA replication differentially depending on the viral genotypes but that the effect was not simply explained by the genotypic background. Perhaps, the effect of the S2204I mutation on HCV replication reflects both intra- and intergenic interactions of NS5A protein. J. Med. Virol. 89:146-152, 2017. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

  1. Cultural differences in crewmembers and mission control personnel during two space station programs.

    PubMed

    Boyd, Jennifer E; Kanas, Nick A; Salnitskiy, Vyacheslav P; Gushin, Vadim I; Saylor, Stephanie A; Weiss, Daniel S; Marmar, Charles R

    2009-06-01

    Cultural differences among crewmembers and mission control personnel can affect long-duration space missions. We examine three cultural contrasts: national (American vs. Russian); occupational (crewmembers vs. mission control personnel); and organizational [Mir space station vs. International Space Station (ISS)]. The Mir sample included 5 American astronauts, 8 Russian cosmonauts, and 42 American and 16 Russian mission control personnel. The ISS sample included 8 astronauts, 9 cosmonauts, and 108 American and 20 Russian mission control personnel. Subjects responded to mood and group climate questions on a weekly basis. The ISS sample also completed a culture and language questionnaire. Crewmembers had higher scores on cultural sophistication than mission control personnel, especially American mission control. Cultural sophistication was not related to mood or social climate. Russian subjects reported greater language flexibility than Americans. Crewmembers reported better mood states than mission control, but both were in the healthy range. There were several Russian-American differences in social climate, with the most robust being higher work pressure among Americans. Russian-American social climate differences were also found in analyses of crew only. Analyses showed Mir-ISS differences in social climate among crew but not in the full sample. We found evidence for national, occupational, and organizational cultural differences. The findings from the Mir space station were essentially replicated on the ISS. Alterations to the ISS to make it a more user-friendly environment have still not resolved the issue of high levels of work pressure among the American crew.

  2. Cultural Perspectives Toward Language Learning

    ERIC Educational Resources Information Center

    Lin, Li-Li

    2008-01-01

    Cultural conflicts may be derived from using inappropriate language. Appropriate linguistic-pragmatic competence may also be produced by providing various and multicultural backgrounds. Culture and language are linked together naturally, unconsciously, and closely in daily social lives. Culture affects language and language affects culture through…

  3. Cross-Cultural Comparison of Self-Construal and Well-Being between Japan and South Korea: The Role of Self-Focused and Other-Focused Relational Selves.

    PubMed

    Park, Joonha; Norasakkunkit, Vinai; Kashima, Yoshi

    2017-01-01

    Relational self, along with individual and collective selves, is a fundamental aspect that makes up self-concept. Proposing its two aspects: self-focused relational self (i.e., perceiving the self as the object of other people's referential awareness or intentionality) and other-focused relational self (i.e., perceiving the self as being attuned and empathetically connected to close others), the current study explored the way the four selves affect well-being in Japan and South Korea, the East Asian cultures that have been assumed to be homogeneously collectivistic in previous psychological literature. Japanese and Korean participants rated a set of well-being and self-related scales. There were visible sample differences within culture by collection method (classroom vs. online) in relative degrees of selves and related constructs, possibly associated with generational differences. Other-focused relational self was greater in the Korean classroom sample than the Japanese counterpart, whereas no difference was found between the online samples. On the other hand, it was consistent between cultures that the two types of relational self showed different associations with social anxiety and self-esteem as expected, and that they predicted well-being in different ways. We discuss implications for the generational differences and their interactions with culture and the importance of separating the two aspects of relational self in the study of self and culture.

  4. The effect of three culture methods on intensive culture system of pacific white shrimp ( Litopenaeus vannamei)

    NASA Astrophysics Data System (ADS)

    Ma, Zhen; Wan, Rong; Song, Xiefa; Gao, Lei

    2013-09-01

    Different culture methods may affect the intensive culture system of Pacific white shrimp ( Litopenaeus vannamei) regarding water quality and growth and economic performance. This study evaluated the potential effects of three culture methods through cultivation of juvenile shrimps under consistent tank management conditions for 84 d. The three methods involved shrimp cultivation in different tanks, i.e., outdoor tanks with cement bottom (mode-C), greenhouse tanks with cement bottom (mode-G) and outdoor tanks with mud-substrate (mode-M). Results showed that water temperature was significantly higher in mode-G than that in mode-C ( P < 0.05). In contrast to the other two treatments, mode-M had stable pH after 50 d cultivation of shrimps. In the mid-late period, the average concentrations of TAN, NO2-N, DIP and COD were significantly lower in mode-M and mode-G compared with those in mode-C ( P < 0.05). Despite lack of differences in the final shrimp weight among different treatments ( P > 0.05), mode-M had significantly higher shrimp yield, survival rate and feed conversion rate ( P < 0.05) than other modes. There were significant differences in revenue and net return among different treatments ( P < 0.05). These demonstrated that the treatments of mode-G and mode-M were conductive to the intensive culture system of L. vannamei.

  5. Composition of commercial media used for human embryo culture.

    PubMed

    Morbeck, Dean E; Krisher, Rebecca L; Herrick, Jason R; Baumann, Nikola A; Matern, Dietrich; Moyer, Thomas

    2014-09-01

    To determine the composition of commercially available culture media and test whether differences in composition are biologically relevant in a murine model. Experimental laboratory study. University-based laboratory. Cryopreserved hybrid mouse one-cell embryos were used in experiments. Amino acid, organic acid, ions, and metal content were determined for two different lots of media from Cook, In Vitro Care, Origio, Sage, Vitrolife, Irvine CSC, and Global. To determine whether differences in the composition of these media are biologically relevant, mouse one-cell embryos were thawed and cultured for 120 hours in each culture media at 5% and 20% oxygen in the presence or absence of protein in an EmbryoScope time-lapse incubator. The compositions of seven culture media were analyzed for concentrations of 39 individual amino acids, organic acids, ions, and elements. Blastocyst rates and cell cycle timings were calculated at 96 hours of culture, and the experiments were repeated in triplicate. Of the 39 analytes, concentrations of glucose, lactate, pyruvate, amino acids, phosphate, calcium, and magnesium were present in variable concentrations, likely reflecting differences in the interpretation of animal studies. Essential trace elements, such as copper and zinc, were not detected. Mouse embryos failed to develop in one culture medium and were differentially affected by oxygen in two other media. Culture media composition varies widely, with differences in pyruvate, lactate, and amino acids especially notable. Blastocyst development was culture media dependent and showed an interaction with oxygen concentration and presence of protein. Copyright © 2014 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

  6. A Cross-Cultural Longitudinal Examination of the Effect of Cumulative Adversity on the Mental and Physical Health of Older Adults

    PubMed Central

    Palgi, Yuval; Shrira, Amit

    2015-01-01

    Self-oriented adversity refers to traumatic events that primarily inflict the self, whereas other-oriented adversity refers to events that affect the self by primarily targeting others. The present study aimed to examine whether cultural background moderates the effects of self-oriented and other-oriented adversity on mental and physical health of older adults. Using longitudinal data from the Israeli component of the Survey of Health and Retirement, we focused on 370 Jews and 239 Arabs who reported their exposure to various adversities across the lifespan, and completed questionnaires regarding mental and physical health. Results showed that the effect of self-oriented adversity on health did not differ among Jews and Arabs. However, other-oriented adversity showed a stronger effect on Arabs’ mental and physical health than on Jews’ health. Our findings suggest that the accumulation of adverse events that affect the self by primarily targeting others may have a stronger impact in collectivist cultures than in individualist cultures. PMID:25961862

  7. Preparing for an influx of foreign students in technical writing courses: Understanding their background

    NASA Technical Reports Server (NTRS)

    Lipson, C. S.

    1981-01-01

    The cultural and historical influences that students from foreign countries bring with them to technical and report writing classes, in particular those influences that would affect their receipt of instruction in American principles for written prose are examined. The grouping of sentences into idea units and into paragraphs, and the grouping of the paragraphs into whole structures are considered. The different cultures, different habits and norms for handling prose, and the dictates for style and structure and sufficiency and approach are also considered.

  8. Improvement of the respiration efficiency of Lactococcus lactis by decreasing the culture pH.

    PubMed

    Shi, Weijia; Li, Yu; Gao, Xueling; Fu, Ruiyan

    2016-03-01

    The growth characteristics and intracellular hemin concentrations of Lactococcus lactis grown under different culture pH and aeration conditions were examined to investigate the effect of culture pH on the respiration efficiency of L. lactis NZ9000 (pZN8148). Cell biomass and biomass yield of L. lactis grown with 4 μg hemin/ml and O2 were higher than those without aeration when the culture pH was controlled at 5-6.5. The culture pH affected the respiratory efficiency in the following order of pH: 5 > 5.5 > 6 > 6.5; the lag phase increased as the culture pH decreased. Hemin accumulation was sensitive to culture pH. Among the four pH conditions, pH 5.5 was optimal for hemin accumulation in the cells. The highest intracellular hemin level in L. lactis resting cells incubated at different pH saline levels (5-6.5) was at pH 5.5. The respiration efficiency of L. lactis under respiration-permissive conditions increases markedly as the culture pH decreases. These results may help develop high cell-density L. lactis cultures. Thus, this microorganism may be used for industrial applications.

  9. Dispersible oxygen microsensors map oxygen gradients in three-dimensional cell cultures.

    PubMed

    Lesher-Pérez, Sasha Cai; Kim, Ge-Ah; Kuo, Chuan-Hsien; Leung, Brendan M; Mong, Sanda; Kojima, Taisuke; Moraes, Christopher; Thouless, M D; Luker, Gary D; Takayama, Shuichi

    2017-09-26

    Phase fluorimetry, unlike the more commonly used intensity-based measurement, is not affected by differences in light paths from culture vessels or by optical attenuation through dense 3D cell cultures and hydrogels thereby minimizing dependence on signal intensity for accurate measurements. This work describes the use of phase fluorimetry on oxygen-sensor microbeads to perform oxygen measurements in different microtissue culture environments. In one example, cell spheroids were observed to deplete oxygen from the cell-culture medium filling the bottom of conventional microwells within minutes, whereas oxygen concentrations remained close to ambient levels for several days in hanging-drop cultures. By dispersing multiple oxygen microsensors in cell-laden hydrogels, we also mapped cell-generated oxygen gradients. The spatial oxygen mapping was sufficiently precise to enable the use of computational models of oxygen diffusion and uptake to give estimates of the cellular oxygen uptake rate and the half-saturation constant. The results show the importance of integrated design and analysis of 3D cell cultures from both biomaterial and oxygen supply aspects. While this paper specifically tests spheroids and cell-laden gel cultures, the described methods should be useful for measuring pericellular oxygen concentrations in a variety of biomaterials and culture formats.

  10. Using ATTO dyes to probe bacterial interactions with the marine diatom Pseudo-nitzschia.

    NASA Astrophysics Data System (ADS)

    Mehic, S.; Sison-Mangus, M.

    2016-02-01

    Pseudo-nitzschia blooms are known to be highly toxic and detrimental to wildlife. The neurotoxin produced by the algae can ripple through the entire food web creating a direct impact on oceanic life and human-related industries. With coastal blooms increasing in both size and duration in recent years, it is crucial that we uncover more microbial interactions that may affect the toxicity of these blooms. Current harmful algal bloom studies have shown that different bacterial consortia can have a great impact Pseudo-nitzschia physiology. More specifically, research suggests that bacteria affect both growth rates and domoic acid concentrations of laboratory grown cultures. However, these studies do not explore the attachment patterns of these bacteria with the diatom. Bacterial attachment may dictate the different types of interactions between bacteria and the diatoms, a trait that is largely unexplored in the symbiotic interactions between the two organisms. In this study, we seek to identify direct and indirect interactions between four bacteria taxa from different phyla and three different species of Pseudo-nitzschia. Our preliminary scanning electron microscopy and DAPI staining experiments hint at distinct differences in attachment among bacterial taxa. To explore this work further, we aim to employ ATTO dyes and epifluorescent microscopy on both binary and multiple cultures to visualize patterns in attachment. By utilizing ATTO dyes with distinct wavelength emissions, we can perform a series experiment that highlights the interaction between bacteria and diatoms, without inserting a fluorescent reporter gene in the bacteria. Multiple cultures will be used to identify possible cooperative or negative interactive traits between bacteria that can affect diatom host physiology. Implications on both phytoplankton physiology and nutrient cycling will be subsequently discussed.

  11. In vitro culture of bovine embryos in murine ES cell conditioned media negatively affects expression of pluripotency-related markers OCT4, SOX2 and SSEA1.

    PubMed

    Oliveira, C S; de Souza, M M; Saraiva, N Z; Tetzner, T A D; Lima, M R; Lopes, F L; Garcia, J M

    2012-06-01

    Despite extensive efforts, establishment of bovine embryonic stem (ES) cell lines has not been successful. We hypothesized that culture conditions for in vitro-produced (IVP) embryos, the most used source of inner cell mass (ICM) to obtain ES cells, might affect their undifferentiated state. Therefore, the aim of this work was to improve pluripotency of IVP blastocysts to produce suitable ICM for further culturing. We tested KSR and foetal calf serum (FCS) supplements in SOF medium and ES cell conditioned medium (CM) on IVC (groups: KSR, KSR CM, FCS and FCS CM). Cleavage and blastocyst rates were similar between all groups. Also, embryonic quality, assessed by apoptosis rates (TUNEL assay), total cell number and ICM percentage did not differ between experimental groups. However, expression of pluripotency-related markers was affected. We detected down-regulation of OCT3/4, SOX2 and SSEA1 in ICM of FCS CM blastocysts (p < 0.05). SOX2 gene expression revealed lower levels (p < 0.05) on KSR CM blastocysts and a remarkable variation in SOX2 mRNA levels on FCS-supplemented blastocysts. In conclusion, pluripotency-related markers tend to decrease after supplementation with ES cell CM, suggesting different mechanisms regulating mouse and bovine pluripotency. KSR supplementation did not differ from FCS, but FCS replacement by KSR may produce blastocysts with stable SOX2 gene expression levels. © 2011 Blackwell Verlag GmbH.

  12. Cytotoxic effects of nanosilver are highly dependent on the chloride concentration and the presence of organic compounds in the cell culture media.

    PubMed

    Kaiser, Jean-Pierre; Roesslein, Matthias; Diener, Liliane; Wichser, Adrian; Nowack, Bernd; Wick, Peter

    2017-01-06

    Nanosilver shows great promise for use in industrial, consumer or medical products because of its antimicrobial properties. However, the underlying mechanisms of the effects of silver nanoparticles on human cells are still controversial. Therefore, in the present study the influence of the chloride concentration and different serum content of culture media on the cytotoxic effects of nanosilver was systematically evaluated. Our results show that nanosilver toxicity was strongly affected by the composition of the culture media. The chloride concentration, as well as the carbon content affected the silver agglomeration and the complex formation. But also the dissolution of nanosilver and the availability of free silver ions (Ag + ) were severely affected by the compositions of the culture media. Cells, only exposed to silver particles in suspension and dissolved silver complexes, did not show any effects under all conditions. Nanosilver agglomerates and silver complexes were not very soluble. Thus, cells growing on the bottom of the culture dishes were exposed to sedimented nanosilver agglomerates and precipitated silver complexes. Locally, the concentration of silver on the cell surface was very high, much higher compared the silver concentration in the bulk solution. The cytotoxic effects of nanosilver are therefore a combination of precipitated silver complexes and organic silver compounds rather than free silver ions. Silver coatings are used in health care products due to their bacteriostatic or antibacterial properties. The assessment of the toxicity of a certain compound is mostly done using in vitro assays. Therefore, cytotoxicity studies of nanosilver using human cell cultures have to be undertaken under well controlled and understood cultivations conditions in order to improve the compatibility of different studies. Especially when eukaryotic versus prokaryotic systems are compared for the evaluation of the use of nanosilver as antibacterial coatings for implants in order to prevent bacterial colonization.

  13. CulSim: A simulator of emergence and resilience of cultural diversity

    NASA Astrophysics Data System (ADS)

    Ulloa, Roberto

    CulSim is an agent-based computer simulation software that allows further exploration of influential and recent models of emergence of cultural groups grounded in sociological theories. CulSim provides a collection of tools to analyze resilience of cultural diversity when events affect agents, institutions or global parameters of the simulations; upon combination, events can be used to approximate historical circumstances. The software provides a graphical and text-based user interface, and so makes this agent-based modeling methodology accessible to a variety of users from different research fields.

  14. [Effective productions of plant secondary metabolites having antitumor activity by plant cell and tissue cultures].

    PubMed

    Taniguchi, Shoko

    2005-06-01

    Methods for the effective production of plant secondary metabolites with antitumor activity using plant cell and tissue cultures were developed. The factors in tannin productivity were investigated using culture strains producing different types of hydrolyzable tannins, i.e., gallotannins (mixture of galloylglucoses), ellagi-, and dehydroellagitannins. Production of ellagi- and dehydroellagitannins was affected by the concentrations and ratio of nitrogen sources in the medium. The formation of oligomeric ellagitannins in shoots of Oenothera tetraptera was correlated with the differentiation of tissues. Cultured cells of Eriobotrya japonica producing ursane- and oleanane-type triterpenes with antitumor activities were also established.

  15. [Functional somatic syndromes from the view of cultural anthropology].

    PubMed

    Nakagami, Ayako; Tsujiuchi, Takuya

    2009-09-01

    The functional somatic syndromes have acquired major socio-cultural and political dimensions. Socio-cultural factors clearly affect symptoms, suffering, and disability perception and reporting. And knowledge of explanatory models of bodily distress for patients from different cultural backgrounds is useful in the establishment of a stable doctor -patient relationship. FSS may be an operational category to bridge between medical explanatory model and patient's model. According to medical anthropology, sickness has two faces; illness and disease. "Disease" is the problem from the practitioner's perspective, and "illness" is the human experience of symptoms and suffering. In this paper, the anthropological research on chronic fatigue syndrome as "not real" illness experience was described.

  16. Different in vitro cellular responses to tamoxifen treatment in polydimethylsiloxane-based devices compared to normal cell culture.

    PubMed

    Wang, Lingyu; Yu, Linfen; Grist, Samantha; Cheung, Karen C; Chen, David D Y

    2017-11-15

    Cell culture systems based on polydimethylsiloxane (PDMS) microfluidic devices offer great flexibility because of their simple fabrication and adaptability. PDMS devices also make it straightforward to set up parallel experiments and can facilitate process automation, potentially speeding up the drug discovery process. However, cells grown in PDMS-based systems can develop in different ways to those grown with conventional culturing systems because of the differences in the containers' surfaces. Despite the growing number of studies on microfluidic cell culture devices, the differences in cellular behavior in PDMS-based devices and normal cell culture systems are poorly characterized. In this work, we investigated the proliferation and autophagy of MCF7 cells cultured in uncoated and Parylene-C coated PDMS wells. Using a quantitative method combining solid phase extraction and liquid chromatography mass spectrometry we developed, we showed that Tamoxifen uptake into the surfaces of uncoated PDMS wells can change the drug's effective concentration in the culture medium, affecting the results of Tamoxifen-induced autophagy and cytotoxicity assays. Such changes must be carefully analyzed before transferring in vitro experiments from a traditional culture environment to a PDMS-based microfluidic system. We also found that cells cultured in Parylene-C coated PDMS wells showed similar proliferation and drug response characteristics to cells cultured in standard polystyrene (PS) plates, indicating that Parylene-C deposition offers an easy way of limiting the uptake of small molecules into porous PDMS materials and significantly improves the performance of PDMS-based device for cell related research. Copyright © 2017 Elsevier B.V. All rights reserved.

  17. The Effect of Preoperative Antimicrobial Prophylaxis on Intraoperative Culture Results in Patients with a Suspected or Confirmed Prosthetic Joint Infection: a Systematic Review

    PubMed Central

    Benito, Natividad; Soriano, Alex

    2017-01-01

    ABSTRACT Obtaining reliable cultures during revision arthroplasty is important to adequately diagnose and treat a prosthetic joint infection (PJI). The influence of antimicrobial prophylaxis on culture results remains unclear. Since withholding prophylaxis increases the risk for surgical site infections, clarification on this topic is critical. A systematic review was performed with the following research question: in patients who undergo revision surgery of a prosthetic joint, does preoperative antimicrobial prophylaxis affect the culture yield of intraoperative samples in comparison with nonpreoperative antimicrobial prophylaxis? Seven articles were included in the final analysis. In most studies, standard diagnostic culture techniques were used. In patients with a PJI, pooled analysis showed a culture yield of 88% (145/165) in the prophylaxis group versus 95% (344/362) in the nonprophylaxis group (P = 0.004). Subanalysis of patients with chronic PJIs showed positive cultures in 88% (78/89) versus 91% (52/57), respectively (P = 0.59). In patients with a suspected chronic infection, a maximum difference of 4% in culture yield between the prophylaxis and nonprophylaxis groups was observed. With the use of standard culture techniques, antimicrobial prophylaxis seems to affect cultures in a minority of patients. Along with the known risk of surgical site infections due to inadequate timing of antimicrobial prophylaxis, we discourage the postponement of prophylaxis until tissue samples are obtained in revision surgery. Future studies are necessary to conclude whether the small percentage of false-negative cultures after prophylaxis can be further reduced with the use of more-sensitive culture techniques, like sonication. PMID:28659322

  18. From self-construal to threat appraisal: Understanding cultural differences in social anxiety between Asian Americans and European Americans.

    PubMed

    Krieg, Alexander; Xu, Yiyuan

    2018-05-21

    Abundant research has shown that Asian Americans tend to score higher on standardized social anxiety measures than do European Americans. The current study explored how this cultural difference in social anxiety may be explained by cultural differences in self-construals and threat appraisal between Asian Americans and European Americans. Participants were 310 Asian Americans and 249 European Americans recruited from a large university in Hawaii who completed questionnaires online in exchange for course credit. Using structural equation modeling, we compared a series of competing models in which cultural differences in social anxiety were specified to be mediated by cultural differences in self-construals and threat appraisal. Compared with European Americans, Asian Americans scored higher on measures of social anxiety, threat appraisal, and interdependent self-construal, and lower on independent self-construal. We found support for the mediating roles of both self-construals and threat appraisal. Specifically, higher interdependent and lower independent self-construal reported by Asian Americans were related to higher threat appraisal, which were, in turn, associated with higher social anxiety. Higher interdependent and lower independent self-construal were also directly related to higher social anxiety over and above the influence of threat appraisal. These findings provide initial evidence on how cultural group differences in beliefs about the self in relation to others may shape what is attended to in a social situation, and contribute to cultural differences in subsequent affective response between Asian Americans and European Americans. (PsycINFO Database Record (c) 2018 APA, all rights reserved).

  19. The health of a nation predicts their mate preferences: cross-cultural variation in women's preferences for masculinized male faces.

    PubMed

    DeBruine, Lisa M; Jones, Benedict C; Crawford, John R; Welling, Lisa L M; Little, Anthony C

    2010-08-07

    Recent formulations of sexual selection theory emphasize how mate choice can be affected by environmental factors, such as predation risk and resource quality. Women vary greatly in the extent to which they prefer male masculinity and this variation is hypothesized to reflect differences in how women resolve the trade-off between the costs (e.g. low investment) and benefits (e.g. healthy offspring) associated with choosing a masculine partner. A strong prediction of this trade-off theory is that women's masculinity preferences will be stronger in cultures where poor health is particularly harmful to survival. We investigated the relationship between women's preferences for male facial masculinity and a health index derived from World Health Organization statistics for mortality rates, life expectancies and the impact of communicable disease. Across 30 countries, masculinity preference increased as health decreased. This relationship was independent of cross-cultural differences in wealth or women's mating strategies. These findings show non-arbitrary cross-cultural differences in facial attractiveness judgements and demonstrate the use of trade-off theory for investigating cross-cultural variation in women's mate preferences.

  20. Culture modulates the brain response to human expressions of emotion: electrophysiological evidence.

    PubMed

    Liu, Pan; Rigoulot, Simon; Pell, Marc D

    2015-01-01

    To understand how culture modulates on-line neural responses to social information, this study compared how individuals from two distinct cultural groups, English-speaking North Americans and Chinese, process emotional meanings of multi-sensory stimuli as indexed by both behaviour (accuracy) and event-related potential (N400) measures. In an emotional Stroop-like task, participants were presented face-voice pairs expressing congruent or incongruent emotions in conditions where they judged the emotion of one modality while ignoring the other (face or voice focus task). Results indicated that while both groups were sensitive to emotional differences between channels (with lower accuracy and higher N400 amplitudes for incongruent face-voice pairs), there were marked group differences in how intruding facial or vocal cues affected accuracy and N400 amplitudes, with English participants showing greater interference from irrelevant faces than Chinese. Our data illuminate distinct biases in how adults from East Asian versus Western cultures process socio-emotional cues, supplying new evidence that cultural learning modulates not only behaviour, but the neurocognitive response to different features of multi-channel emotion expressions. Copyright © 2014 Elsevier Ltd. All rights reserved.

  1. Cultural change and loss of ethnoecological knowledge among the Isthmus Zapotecs of Mexico.

    PubMed

    Saynes-Vásquez, Alfredo; Caballero, Javier; Meave, Jorge A; Chiang, Fernando

    2013-06-11

    Global changes that affect local societies may cause the loss of ecological knowledge. The process of cultural change in Zapotec communities of the Oaxacan Isthmus intensified during the first years of the 20th century due to industrial and agro-industrial modernization projects and an increase in the level of formal schooling. Based on the case of the Oaxacan Isthmus, this study assesses the relationship between cultural change and the loss of traditional ecological knowledge (TEK). Three hundred male heads of family were interviewed from three municipalities in the Isthmus of Tehuantepec, Oaxaca, Mexico selected to span a wide range of cultural change. Each participant was shown herbarium specimens and photographs of a sample of 30 species drawn from a pool of 94 representing local plant diversity. Visual recognition of each species, knowledge of plant form, generic name, specific name, and local uses were scored. The sum of the five scores provided an index of global knowledge which we used as a proxy for TEK. Analysis of variance revealed differences between groups of economic activities. We collected socio-demographic data from the interviewees such as age, level of schooling, and competency in the local language. With these data we ran a principal component analysis and took the first axis as an index of cultural change, and correlated it with the scores obtained each respondent. We found statistically significant differences between groups of people with different economic activities, as well as a highly significant negative relationship between the Index of cultural change and ecological knowledge at all levels, with regression coefficients between 81.2% and 88.3%, indicating that cultural change is affecting traditional botanical knowledge. Our results shown that cultural change, as indicated by occupational activity, level of formal schooling, and competence in the indigenous language, is negatively associated with the loss of Zapotec ethnobotanical knowledge. Heads of family engaged in secondary economic activities and services were less culturally competent, especially regarding the knowledge of generic and specific names as well as plant uses.

  2. Cultural change and loss of ethnoecological knowledge among the Isthmus Zapotecs of Mexico

    PubMed Central

    2013-01-01

    Background Global changes that affect local societies may cause the loss of ecological knowledge. The process of cultural change in Zapotec communities of the Oaxacan Isthmus intensified during the first years of the 20th century due to industrial and agro-industrial modernization projects and an increase in the level of formal schooling. Based on the case of the Oaxacan Isthmus, this study assesses the relationship between cultural change and the loss of traditional ecological knowledge (TEK). Methods Three hundred male heads of family were interviewed from three municipalities in the Isthmus of Tehuantepec, Oaxaca, Mexico selected to span a wide range of cultural change. Each participant was shown herbarium specimens and photographs of a sample of 30 species drawn from a pool of 94 representing local plant diversity. Visual recognition of each species, knowledge of plant form, generic name, specific name, and local uses were scored. The sum of the five scores provided an index of global knowledge which we used as a proxy for TEK. Analysis of variance revealed differences between groups of economic activities. We collected socio-demographic data from the interviewees such as age, level of schooling, and competency in the local language. With these data we ran a principal component analysis and took the first axis as an index of cultural change, and correlated it with the scores obtained each respondent. Results We found statistically significant differences between groups of people with different economic activities, as well as a highly significant negative relationship between the Index of cultural change and ecological knowledge at all levels, with regression coefficients between 81.2% and 88.3%, indicating that cultural change is affecting traditional botanical knowledge. Conclusions Our results shown that cultural change, as indicated by occupational activity, level of formal schooling, and competence in the indigenous language, is negatively associated with the loss of Zapotec ethnobotanical knowledge. Heads of family engaged in secondary economic activities and services were less culturally competent, especially regarding the knowledge of generic and specific names as well as plant uses. PMID:23758714

  3. Cultutal Factors Affecting English Proficiency in Rural Areas

    ERIC Educational Resources Information Center

    Ler, Ee Chop

    2012-01-01

    The purpose of this study is to investigate the rural "cultural" problems and to determine their effect on the learning of English. Twenty students from different ethnic backgrounds and English language proficiency in six rural schools in Terengganu, Malaysia were interviewed. In addition the teachers also from different rural schools…

  4. Comparative Studies of Cognitive Styles: Implications for the Education of Immigrant Students.

    ERIC Educational Resources Information Center

    Berry, John W.

    Differences in cognitive styles or intellectual behavior can be attributed to different ecological and cultural influences on individuals. These influences, such as type of subsistence economy, social stratification, socialization, and role specialization, affect the development of a particular cognitive style which is determined by whether an…

  5. Discourse Strategies of Italian and English Sales Promotion Letters

    ERIC Educational Resources Information Center

    Vergaro, Carla

    2004-01-01

    This article describes a contrastive study on rhetorical differences between Italian and English sales promotion letters. It is assumed that cultural differences affect discourse genres traditionally considered as standardized, ritual or even formulaic, written business communication being a case in point. It was our goal to investigate how…

  6. Anger Elicitation in Tonga and Germany: The Impact of Culture on Cognitive Determinants of Emotions

    PubMed Central

    Bender, Andrea; Spada, Hans; Rothe-Wulf, Annelie; Traber, Simone; Rauss, Karsten

    2012-01-01

    The cognitive appraisal of an event is crucial for the elicitation and differentiation of emotions, and causal attributions are an integral part of this process. In an interdisciplinary project comparing Tonga and Germany, we examined how cultural differences in attribution tendencies affect emotion assessment and elicitation. Data on appraising causality and responsibility and on emotional responses were collected through questionnaires based on experimentally designed vignettes, and were related to culture-specific values, norms, and the prevailing self-concept. The experimental data support our hypothesis that – driven by culturally defined self-concepts and corresponding attribution tendencies – members of the two cultures cognitively appraise events in diverging manners and consequently differ in their emotional responses. Ascription of responsibility to self and/or circumstances, in line with a more interdependent self-concept, co-varies with higher ratings of shame, guilt, and sadness, whereas ascription of responsibility to others, in line with a less interdependent self-concept, co-varies with higher ratings of anger. These findings support the universal contingency hypothesis and help to explain cultural differences in this domain on a fine-grained level. PMID:23112780

  7. Control of interferon-tau secretion by in vitro-derived bovine blastocysts during extended culture and outgrowth formation.

    PubMed

    Kubisch, H M; Larson, M A; Kiesling, D O

    2001-04-01

    A series of experiments was conducted to examine the pattern of interferon-tau (IFN-tau) secretion by bovine blastocysts during extended culture in vitro. In the first experiment, blastocysts were cultured individually for three 48-hour periods. The day of blastocyst formation affected how much IFN-tau was produced during the first two culture periods, but not during the third period. The overall secretion of IFN-tau during the 6-day period increased significantly and well beyond what could be accounted for by the concomitant increase in cell numbers. In the second experiment, blastocysts were initially cultured in individual droplets for 48 hr, then plated into 48-well plates. Medium concentrations of IFN-tau were determined after 48 hr and again after 6 and 12 days of culture. Initial IFN-tau secretion did not affect the ability to form outgrowths or their final size, and initial differences in secretion between groups of blastocysts had disappeared by the second and third analyses. In the third experiment, blastocysts were cultured individually for 48 hr in droplets containing the medium that had been flushed through the uteri of non-pregnant sheep on days 10, 12, and 15 of the estrous cycle. Culture in the medium obtained from the Day 15 flush significantly increased the number of cells that blastocysts contained, as well as IFN-tau secretion.

  8. Dissolution of man-made vitreous fibers in rat alveolar macrophage culture and Gamble's saline solution: influence of different media and chemical composition of the fibers.

    PubMed Central

    Luoto, K; Holopainen, M; Karppinen, K; Perander, M; Savolainen, K

    1994-01-01

    The effect of different chemical compositions of man-made vitreous fibers (MMVF) on their dissolution by alveolar macrophages (AM) in culture and in Gamble's solution was studied. The fibers were exposed to cultured rat AMs, culture medium alone; or Gamble's saline solution for 2, 4, or 8 days. The dissolution of the fibers was studied by measuring the amount of silicon (Si), iron (Fe), and aluminum (Al) in each medium. The AMs in culture dissolved Fe and Al from the fibers but the dissolution of Si was more marked in the cell culture medium without cells and in the Gamble's solution. The dissolution of Si, Fe, and Al was different for different fibers, and increased as a function of time. The Fe and Al content of the fibers correlated negatively with the dissolution of Si by AMs from the MMVF, i.e., when the content of Fe and Al of the fibers increased the dissolution of Si decreased. These results suggest that the chemical composition of MMVFs has a marked effect on their dissolution. AMs seem to affect the dissolution of Fe and Al from the fibers. This suggests that in vitro models with cells in the media rather than only culture media or saline solutions would be preferable in dissolution studies of MMVFs. PMID:7882911

  9. Cross-Cultural Differences in Children’s Choices, Categorizations, and Evaluations of Truths and Lies

    PubMed Central

    Fu, Genyue; Xu, Fen; Cameron, Catherine Ann; Heyman, Gail; Lee, Kang

    2008-01-01

    This study examined cross-cultural differences and similarities in children’s moral understanding of individual- or collective-oriented lies and truths. Seven-, 9-, and 11-year-old Canadian and Chinese children were read stories about story characters facing moral dilemmas about whether to lie or tell the truth to help a group but harm an individual or vice versa. Participants chose to lie or to tell the truth as if they were the character (Experiments 1 and 2) and categorized and evaluated the story characters’ truthful and untruthful statements (Experiments 3 and 4). Most children in both cultures labeled lies as lies and truths as truths. The major cultural differences lay in choices and moral evaluations. Chinese children chose lying to help a collective but harm an individual, and they rated it less negatively than lying with opposite consequences. Chinese children rated truth telling to help an individual but harm a group less positively than the alternative. Canadian children did the opposite. These findings suggest that cross-cultural differences in emphasis on groups versus individuals affect children’s choices and moral judgments about truth and deception. PMID:17352539

  10. Culture conditions affect cytotoxin production by Serratia marcescens.

    PubMed

    Carbonell, G V; Fonseca, B A; Figueiredo, L T; Darini, A L; Yanaguita, R M

    1996-12-31

    Cytotoxins have been implicated in the pathogenesis of bacterial infections. In this study, the influence of different culture conditions was evaluated on cytotoxin production of Serratia marcescens. Parameters such as culture media, incubation temperature, starting pH of culture medium, aeration, anaerobiosis, carbon sources, iron concentration in he culture media, and release of cell-bond toxin by polymyxin B were investigated. The data suggest that this cytotoxin is predominantly extracellular and is not induced by iron limitation. Aerobic culture with shaking resulted in higher cytotoxicity than static aerobic or anaerobic culture. Bacteria grown in glucose, sucrose or galactose were more cytotoxic than those grown in inositol or maltose. The culture conditions that were identified as optimal for cytotoxin production by Serratia marcescens were incubation temperature ranging from 30 to 37 degrees C, in medium adjusted pH 8.5, with shaking. This work will contribute to further studies on the identification of this cytotoxic activity.

  11. 30 CFR 942.774 - Revision; renewal; and transfer, assignment, or sale of permit rights.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... result in adverse impacts beyond those previously considered, affecting cultural resources listed on, or... change in the identification, disturbance, or handling of toxic- or acid-forming materials different from...

  12. One's Colonies: a virtual reality environment of oriental residences

    NASA Astrophysics Data System (ADS)

    Chi, Catherine

    2013-03-01

    This paper is a statement about my virtual reality environment project, One's Colonies, and a description of the creative process of the project. I was inspired by the buildings in my hometown-Taiwan, which is really different from the architectural style in the United States. By analyzing the unique style of dwellings in Taiwan, I want to demonstrate how the difference between geography, weather and culture change the appearance of the living space. Through this project I want to express the relationship between architectural style and cultural difference, and how the emotional condition or characteristics of the residents are affected by their residencies.

  13. Factors affecting job satisfaction of Aboriginal mental health workers working in community mental health in rural and remote New South Wales.

    PubMed

    Cosgrave, Catherine; Maple, Myfanwy; Hussain, Rafat

    2017-12-01

    Objective The aim of the present study was to identify factors affecting the job satisfaction and subsequent retention of Aboriginal mental health workers (AMHWs). Methods Five AMHWs working in New South Wales (NSW) for NSW Health in rural and remote community mental health (CMH) services participated in in-depth, semi-structured interviews to understand how employment and rural living factors affected workers' decisions to stay or leave their CMH positions. Results Using a constructivist grounded theory analysis, three aspects negatively impacting the job satisfaction of AMHWs were identified: (1) difficulties being accepted into the team and organisation; (2) culturally specific work challenges; and (3) professional differences and inequality. Conclusions Policy and procedural changes to the AMHW training program may address the lower remuneration and limited career opportunities identified with regard to the Bachelor Health Sciences (Mental Health) qualification. Delivering training to increase levels of understanding about the AMHW training program, and cultural awareness generally, to CMH staff and NSW Health management may assist in addressing the negative team, organisational and cultural issues identified. What is known about the topic? The Bachelor Health Sciences (Mental Health) qualification and traineeship pathway undertaken by AMHWs differs significantly from that of other health professionals working in NSW Health's CMH services. The health workforce literature identifies that each health professional group has its own culture and specific values and that forming and maintaining a profession-specific identity is an extremely important aspect of job satisfaction for health workers. What does the paper add? AMHWs working in rural and remote NSW CMH services commonly experience low levels of job satisfaction, especially while undertaking the embedded training program. Of particular concern is the health sciences qualification not translating into NSW Health's 'professionalised' workplace, as well as having negative effects with regard to remuneration and career opportunities within NSW Health. In addition, role challenges involving cultural differences and managing additional professional and personal boundaries negatively affects the job satisfaction of AMHWs. What are the implications for practice? The current structure of the AMHW training program creates workplace conditions that contribute to job dissatisfaction among rural and remote-based AMHWs. Many issues could be rectified by NSW Health making changes to the degree qualification obtained under the training program, as well as raising the level of understanding about the program and Indigenous cultural awareness generally among CMH staff and NSW Health management.

  14. Exploring the relevance of autonomy and relatedness for mental health in healthy and depressed women from two different cultures: when does culture matter?

    PubMed

    Balkir, Nazli; Arens, Elisabeth A; Barnow, Sven

    2013-08-01

    It is well known that the absence of both autonomy and social support (relatedness) are two important etiologic pathways to major depressive disorder (MDD). However, cross-cultural researchers state that the implications of autonomy and relatedness for mental health vary across cultures. To test these assumptions, the current study investigated the relevance of autonomy and relatedness for mental health in healthy and depressed women from two different cultures (Germans and Turkish immigrants in Germany). One hundred and eight (108) women were evaluated for their levels of autonomy/relatedness satisfaction, for overall psychopathological complaints including depression, for affectivity and for perceived loneliness through self-report measures. Among healthy groups, relatedness satisfaction predicted better mental health in Turkish women, whereas in German women, autonomy satisfaction was the better mental health predictor. Within depressed groups however, cultural differences in mental health outcomes regarding autonomy were no longer evident. Autonomy was associated with higher levels of mental health in Turkish as well as in German patients. Our findings indicate that the relationship between autonomy and mental health is culture-specific in healthy women, but disappears in depressed women. These findings are discussed with consideration of clinical implications and an outlook regarding further research.

  15. Cultural Transmission on the Taskscape: Exploring the Effects of Taskscape Visibility on Cultural Diversity

    PubMed Central

    Premo, L. S.; Tostevin, Gilbert B.

    2016-01-01

    Culturally transmitted behavior can be structured in its performance both geographically and temporally, in terms of where and when implements are made and used on the landscape (what Ingold calls “the taskscape”). Yet cultural transmission theory has not yet explored the consequences of behaviors transmitted differently due to their enactment at different taskscape locations, what Tostevin calls “taskscape visibility.” Here, we use computer simulations to explore how taskscape visibility and forager mobility affect the diversity of two selectively neutral culturally transmitted traits within a single population of social learners. The trait that can be transmitted from residential bases only (lower taskscape visibility) shows greater diversity than the trait that can be transmitted from residential bases and logistical camps (higher taskscape visibility). In addition, increased logistical mobility has a positive effect on the diversity of the trait with the lower taskscape visibility while it generally shows little to no effect on the diversity of the trait with higher taskscape visibility. Without an appreciation for the ways in which taskscape visibility and mobility can structure cultural transmission in space and through time, the difference in the observed equilibrium diversity levels of the two traits might be incorrectly interpreted as resulting from qualitatively different forms of biased cultural transmission. The results of our simulation experiment suggest that researchers may need to take the taskscape visibility into account when inferring cultural transmission from archaeological data. PMID:27583682

  16. Effects of cultural characteristics on building an emotion classifier through facial expression analysis

    NASA Astrophysics Data System (ADS)

    da Silva, Flávio Altinier Maximiano; Pedrini, Helio

    2015-03-01

    Facial expressions are an important demonstration of humanity's humors and emotions. Algorithms capable of recognizing facial expressions and associating them with emotions were developed and employed to compare the expressions that different cultural groups use to show their emotions. Static pictures of predominantly occidental and oriental subjects from public datasets were used to train machine learning algorithms, whereas local binary patterns, histogram of oriented gradients (HOGs), and Gabor filters were employed to describe the facial expressions for six different basic emotions. The most consistent combination, formed by the association of HOG filter and support vector machines, was then used to classify the other cultural group: there was a strong drop in accuracy, meaning that the subtle differences of facial expressions of each culture affected the classifier performance. Finally, a classifier was trained with images from both occidental and oriental subjects and its accuracy was higher on multicultural data, evidencing the need of a multicultural training set to build an efficient classifier.

  17. Blending genetics and sociocultural historical inquiry: ethics, culture, and human subjects protection in international cross cultural research.

    PubMed

    Sampson, Deborah A; Caldwell, Dennis; Taylor, Andre D; Taylor, Jacquelyn Y

    2013-03-01

    In this paper, we examine the implementation and difficulties when conducting genetics research in a rural, traditional West African culture within the frame of the United States' grounded research ethics. Research challenges are highlighted by Western researchers following U.S. Institutional Review Board (IRB) guidelines and practices in a non-Western country. IRB concepts are culture bound in Western ideals that may not have synchronicity and compatibility with non-Western cultures. Differences in sociocultural norms, traditions, language, and geography were influencing factors that can affect application of IRB principles. Suggestions for change are offered, which will potentially aid researchers considering application of IRB requirements when conducting research in non-Westernized, non-industrialized countries.

  18. Blending Genetics and Sociocultural Historical Inquiry: Ethics, Culture, and Human Subjects Protection in International Cross Cultural Research

    PubMed Central

    Sampson, Deborah A.; Caldwell, Dennis; Taylor, Andre D.; Taylor, Jacquelyn Y.

    2013-01-01

    In this paper, we examine the implementation and difficulties when conducting genetics research in a rural, traditional West African culture within the frame of the United States’ grounded research ethics. Research challenges are highlighted by Western researchers following U.S. Institutional Review Board (IRB) guidelines and practices in a non-Western country. IRB concepts are culture bound in Western ideals that may not have synchronicity and compatibility with non-Western cultures. Differences in sociocultural norms, traditions, language, and geography were influencing factors that can affect application of IRB principles. Suggestions for change are offered, which will potentially aid researchers considering application of IRB requirements when conducting research in non-Westernized, non-industrialized countries. PMID:23482512

  19. Perception of emotional nonsense sentences in China, Egypt, Estonia, Finland, Russia, Sweden, and the USA.

    PubMed

    Waaramaa, Teija

    2015-10-01

    The present study focused on the identification of emotions in cross-cultural conditions on different continents and among subjects with divergent language backgrounds. The aim was to investigate whether the perception of the basic emotions from nonsense vocal samples was universal, dependent on voice quality, musicality, and/or gender. Listening tests for 350 participants were conducted on location in a variety of cultures: China, Egypt, Estonia, Finland, Russia, Sweden, and the USA. The results suggested that the voice quality parameters played a role in the identification of emotions without the linguistic content. Cultural background may affect the interpretation of the emotions more than the presumed universality. Musical interest tended to facilitate emotion identification. No gender differences were found.

  20. Use of autochthonous Pediococcus acidilactici and Staphylococcus vitulus starter cultures in the production of "chorizo" in 2 different traditional industries.

    PubMed

    Casquete, Rocío; Benito, María J; Martín, Alberto; Ruiz-Moyano, Santiago; Aranda, Emilio; Córdoba, María G

    2012-01-01

    The present study determined how the different ripening conditions affected the growth and development of 3 autochthonous starter cultures, and the physico-chemical and sensory characteristics of chorizo. Each of 3 strains of Pediococcus acidilactici (MC184, MS198, and MS200) and one of Staphylococcus vitulus (RS34) were associated to prepare the starter cultures, P184S34, P198S34, and P200S34. Then, chorizo was prepared following 2 manufacturing procedures. The autochthonous starter cultures were able to compete and colonize the sausages in both ripening procedures. The use of the starter cultures showed evident differences by the texture analysis, with the control batches being generally tougher than the starter culture batches. Also, the highest biogenic amine (BA) levels were found in control batches and the lowest in P200S34 batches. While the use of these starter cultures does not change the sensory characteristics of these traditional fermented sausages, it improves their homogeneity and safety, except for P184S34 batch in which more BAs are detected in industry 2. The 3 autochthonous starter cultures selected could be used in traditional industries because they are able to compete well and colonize the dry fermented sausages "chorizo." The use of these starter cultures improves the texture and homogeneity of traditional fermented sausages. Biogenic amines decreased in the starter cultures batches improving the safety. © 2011 Institute of Food Technologists®

  1. Use of ex vivo and in vitro cultures of the human respiratory tract to study the tropism and host responses of highly pathogenic avian influenza A (H5N1) and other influenza viruses.

    PubMed

    Chan, Renee W Y; Chan, Michael C W; Nicholls, John M; Malik Peiris, J S

    2013-12-05

    The tropism of influenza viruses for the human respiratory tract is a key determinant of host-range, and consequently, of pathogenesis and transmission. Insights can be obtained from clinical and autopsy studies of human disease and relevant animal models. Ex vivo cultures of the human respiratory tract and in vitro cultures of primary human cells can provide complementary information provided they are physiologically comparable in relevant characteristics to human tissues in vivo, e.g. virus receptor distribution, state of differentiation. We review different experimental models for their physiological relevance and summarize available data using these cultures in relation to highly pathogenic avian influenza H5N1, in comparison where relevant, with other influenza viruses. Transformed continuous cell-lines often differ in important ways to the corresponding tissues in vivo. The state of differentiation of primary human cells (respiratory epithelium, macrophages) can markedly affect virus tropism and host responses. Ex vivo cultures of human respiratory tissues provide a close resemblance to tissues in vivo and may be used to risk assess animal viruses for pandemic threat. Physiological factors (age, inflammation) can markedly affect virus receptor expression and virus tropism. Taken together with data from clinical studies on infected humans and relevant animal models, data from ex vivo and in vitro cultures of human tissues and cells can provide insights into virus transmission and pathogenesis and may provide understanding that leads to novel therapeutic interventions. Copyright © 2013 Elsevier B.V. All rights reserved.

  2. Respiratory complaints in Chinese: cultural and diagnostic specificities.

    PubMed

    Han, Jiangna; Zhu, Yuanjue; Li, Shunwei; Chen, Xiansheng; Put, Claudia; Van de Woestijne, Karel P; Van den Bergh, Omer

    2005-06-01

    We investigated the qualitative components of a wide range of Chinese descriptors of dyspnea and associated symptoms, and their relevance for clinical diagnosis. Sixty-one spontaneously reported descriptors were elicited in Chinese patients to make a symptom checklist, which was administered to new groups of patients with different cardiopulmonary diseases, to patients with medically unexplained dyspnea and to healthy subjects. Test-retest reliability was satisfactory for most of the descriptors. A principal component analysis on 61 descriptors yielded the following eight factors: dyspnea-effort of breathing; dyspnea-affective aspect; wheezing; anxiety; tingling; palpitation; coughing and sputum; and dying experience. Although the descriptors of dyspnea-effort of breathing resembled Western wordings and were shared by patients with a variety of diseases, the descriptors of dyspnea-affective aspect appeared to be more culturally specific and were primarily linked to the diagnosis of medically unexplained dyspnea, whereas wheezing was specifically linked to asthma. Three factors of breathlessness were found in Chinese. The descriptors of dyspnea-effort of breathing and wheezing appear to be similar to Western descriptors, whereas the dyspnea-affective aspect seems to bear cultural specificity.

  3. Emotion perception across cultures: the role of cognitive mechanisms

    PubMed Central

    Engelmann, Jan B.; Pogosyan, Marianna

    2012-01-01

    Despite consistently documented cultural differences in the perception of facial expressions of emotion, the role of culture in shaping cognitive mechanisms that are central to emotion perception has received relatively little attention in past research. We review recent developments in cross-cultural psychology that provide particular insights into the modulatory role of culture on cognitive mechanisms involved in interpretations of facial expressions of emotion through two distinct routes: display rules and cognitive styles. Investigations of emotion intensity perception have demonstrated that facial expressions with varying levels of intensity of positive affect are perceived and categorized differently across cultures. Specifically, recent findings indicating significant levels of differentiation between intensity levels of facial expressions among American participants, as well as deviations from clear categorization of high and low intensity expressions among Japanese and Russian participants, suggest that display rules shape mental representations of emotions, such as intensity levels of emotion prototypes. Furthermore, a series of recent studies using eye tracking as a proxy for overt attention during face perception have identified culture-specific cognitive styles, such as the propensity to attend to very specific features of the face. Together, these results suggest a cascade of cultural influences on cognitive mechanisms involved in interpretations of facial expressions of emotion, whereby cultures impart specific behavioral practices that shape the way individuals process information from the environment. These cultural influences lead to differences in cognitive styles due to culture-specific attentional biases and emotion prototypes, which partially account for the gradient of cultural agreements and disagreements obtained in past investigations of emotion perception. PMID:23486743

  4. Emotion perception across cultures: the role of cognitive mechanisms.

    PubMed

    Engelmann, Jan B; Pogosyan, Marianna

    2013-01-01

    Despite consistently documented cultural differences in the perception of facial expressions of emotion, the role of culture in shaping cognitive mechanisms that are central to emotion perception has received relatively little attention in past research. We review recent developments in cross-cultural psychology that provide particular insights into the modulatory role of culture on cognitive mechanisms involved in interpretations of facial expressions of emotion through two distinct routes: display rules and cognitive styles. Investigations of emotion intensity perception have demonstrated that facial expressions with varying levels of intensity of positive affect are perceived and categorized differently across cultures. Specifically, recent findings indicating significant levels of differentiation between intensity levels of facial expressions among American participants, as well as deviations from clear categorization of high and low intensity expressions among Japanese and Russian participants, suggest that display rules shape mental representations of emotions, such as intensity levels of emotion prototypes. Furthermore, a series of recent studies using eye tracking as a proxy for overt attention during face perception have identified culture-specific cognitive styles, such as the propensity to attend to very specific features of the face. Together, these results suggest a cascade of cultural influences on cognitive mechanisms involved in interpretations of facial expressions of emotion, whereby cultures impart specific behavioral practices that shape the way individuals process information from the environment. These cultural influences lead to differences in cognitive styles due to culture-specific attentional biases and emotion prototypes, which partially account for the gradient of cultural agreements and disagreements obtained in past investigations of emotion perception.

  5. Do Dutch doctors communicate differently with immigrant patients than with Dutch patients?

    PubMed

    Meeuwesen, Ludwien; Harmsen, Johannes A M; Bernsen, Roos M D; Bruijnzeels, Marc A

    2006-11-01

    The aim of this study was to gain deeper insight into relational aspects of the medical communication pattern in intercultural consultations at GP practices in the Netherlands. We ask whether there are differences in the verbal interaction of Dutch GPs with immigrant and Dutch patients. Data were drawn from 144 adult patient interviews and video observations of consultations between the patients and 31 Dutch GPs. The patient group consisted of 61 non-Western immigrants (Turkish, Moroccan, Surinamese, Antillean, Cape Verdian) and 83 Dutch participants. Affective and instrumental aspects of verbal communication were assessed using Roter's Interaction Analysis System (RIAS). Patients' cultural background was assessed by ethnicity, language proficiency, level of education, religiosity and cultural views (in terms of being more traditional or more modern). Consultations with the non-Western immigrant patients (especially those from Turkey and Morocco) were well over 2 min shorter, and the power distance between GPs and these patients was greater when compared to the Dutch patients. Major differences in verbal interaction were observed on the affective behavior dimensions, but not on the instrumental dimensions. Doctors invested more in trying to understand the immigrant patients, while in the case of Dutch patients they showed more involvement and empathy. Dutch patients seemed to be more assertive in the medical conversation. The differences are discussed in terms of patients' ethnic background, cultural views (e.g. practicing a religion) and linguistic barriers. It is concluded that attention to cultural diversity does matter, as this leads to different medical communication patterns. A two-way strategy is recommended for improving medical communication, with implications for both doctor and patient behavior.

  6. The Associations of Dyadic Coping and Relationship Satisfaction Vary between and within Nations: A 35-Nation Study.

    PubMed

    Hilpert, Peter; Randall, Ashley K; Sorokowski, Piotr; Atkins, David C; Sorokowska, Agnieszka; Ahmadi, Khodabakhsh; Aghraibeh, Ahmad M; Aryeetey, Richmond; Bertoni, Anna; Bettache, Karim; Błażejewska, Marta; Bodenmann, Guy; Borders, Jessica; Bortolini, Tiago S; Butovskaya, Marina; Castro, Felipe N; Cetinkaya, Hakan; Cunha, Diana; David, Oana A; DeLongis, Anita; Dileym, Fahd A; Domínguez Espinosa, Alejandra D C; Donato, Silvia; Dronova, Daria; Dural, Seda; Fisher, Maryanne; Frackowiak, Tomasz; Gulbetekin, Evrim; Hamamcıoğlu Akkaya, Aslıhan; Hansen, Karolina; Hattori, Wallisen T; Hromatko, Ivana; Iafrate, Raffaella; James, Bawo O; Jiang, Feng; Kimamo, Charles O; King, David B; Koç, Fırat; Laar, Amos; Lopes, Fívia De Araújo; Martinez, Rocio; Mesko, Norbert; Molodovskaya, Natalya; Moradi, Khadijeh; Motahari, Zahrasadat; Natividade, Jean C; Ntayi, Joseph; Ojedokun, Oluyinka; Omar-Fauzee, Mohd S B; Onyishi, Ike E; Özener, Barış; Paluszak, Anna; Portugal, Alda; Relvas, Ana P; Rizwan, Muhammad; Salkičević, Svjetlana; Sarmány-Schuller, Ivan; Stamkou, Eftychia; Stoyanova, Stanislava; Šukolová, Denisa; Sutresna, Nina; Tadinac, Meri; Teras, Andero; Tinoco Ponciano, Edna L; Tripathi, Ritu; Tripathi, Nachiketa; Tripathi, Mamta; Vilchinsky, Noa; Xu, Feng; Yamamoto, Maria E; Yoo, Gyesook

    2016-01-01

    Theories about how couples help each other to cope with stress, such as the systemic transactional model of dyadic coping, suggest that the cultural context in which couples live influences how their coping behavior affects their relationship satisfaction. In contrast to the theoretical assumptions, a recent meta-analysis provides evidence that neither culture, nor gender, influences the association between dyadic coping and relationship satisfaction, at least based on their samples of couples living in North America and West Europe. Thus, it is an open questions whether the theoretical assumptions of cultural influences are false or whether cultural influences on couple behavior just occur in cultures outside of the Western world. In order to examine the cultural influence, using a sample of married individuals (N = 7973) from 35 nations, we used multilevel modeling to test whether the positive association between dyadic coping and relationship satisfaction varies across nations and whether gender might moderate the association. RESULTS reveal that the association between dyadic coping and relationship satisfaction varies between nations. In addition, results show that in some nations the association is higher for men and in other nations it is higher for women. Cultural and gender differences across the globe influence how couples' coping behavior affects relationship outcomes. This crucial finding indicates that couple relationship education programs and interventions need to be culturally adapted, as skill trainings such as dyadic coping lead to differential effects on relationship satisfaction based on the culture in which couples live.

  7. Differential Effects of Tissue Culture Coating Substrates on Prostate Cancer Cell Adherence, Morphology and Behavior

    PubMed Central

    Liberio, Michelle S.; Sadowski, Martin C.; Soekmadji, Carolina; Davis, Rohan A.; Nelson, Colleen C.

    2014-01-01

    Weak cell-surface adhesion of cell lines to tissue culture surfaces is a common problem and presents technical limitations to the design of experiments. To overcome this problem, various surface coating protocols have been developed. However, a comparative and precise real-time measurement of their impact on cell behavior has not been conducted. The prostate cancer cell line LNCaP, derived from a patient lymph node metastasis, is a commonly used model system in prostate cancer research. However, the cells’ characteristically weak attachment to the surface of tissue culture vessels and cover slips has impeded their manipulation and analysis and use in high throughput screening. To improve the adherence of LNCaP cells to the culture surface, we compared different coating reagents (poly-l-lysine, poly-l-ornithine, collagen type IV, fibronectin, and laminin) and culturing conditions and analyzed their impact on cell proliferation, adhesion, morphology, mobility and gene expression using real-time technologies. The results showed that fibronectin, poly-l-lysine and poly-l-ornithine improved LNCaP cells adherence and provoked cell morphology alterations, such as increase of nuclear and cellular area. These coating reagents also induced a higher expression of F-actin and reduced cell mobility. In contrast, laminin and collagen type IV did not improve adherence but promoted cell aggregation and affected cell morphology. Cells cultured in the presence of laminin displayed higher mobility than control cells. All the coating conditions significantly affected cell viability; however, they did not affect the expression of androgen receptor-regulated genes. Our comparative findings provide important insight for the selection of the ideal coating reagent and culture conditions for the cancer cell lines with respect to their effect on proliferation rate, attachment, morphology, migration, transcriptional response and cellular cytoskeleton arrangement. PMID:25375165

  8. Factors affecting the relationship between quantitative polymerase chain reaction (qPCR) and culture-based enumeration of Enterococcus in environmental waters.

    PubMed

    Raith, M R; Ebentier, D L; Cao, Y; Griffith, J F; Weisberg, S B

    2014-03-01

    To determine the extent to which discrepancies between qPCR and culture-based results in beach water quality monitoring can be attributed to: (i) within-method variability, (ii) between-method difference within each method class (qPCR or culture) and (iii) between-class difference. We analysed 306 samples using two culture-based (EPA1600 and Enterolert) and two qPCR (Taqman and Scorpion) methods, each in duplicate. Both qPCR methods correlated with EPA1600, but regression analyses indicated approximately 0·8 log10 unit overestimation by qPCR compared to culture methods. Differences between methods within a class were less than half of this and were minimal for between-replicate within a method. Using the 104 Enterococcus per 100 ml management decision threshold, Taqman qPCR indicated the same decisions as EPA1600 for 87% of the samples, but indicated beach posting for unhealthful water when EPA1600 did not for 12% of the samples. After accounting for within-method and within-class variability, 8% of the samples exhibited true between-class discrepancy where both qPCR methods indicated beach posting while both culture methods did not. Measurement target difference (DNA vs growth) accounted for the majority of the qPCR-vs-culture discrepancy, but its influence on monitoring application is outweighed by frequent incorrect posting with culture methods due to incubation time delay. This is the first study to quantify the frequency with which culture-vs-qPCR discrepancies can be attributed to target difference - vs - method variability. © 2013 The Society for Applied Microbiology.

  9. Cultural Diversity, Economic Development and Societal Instability

    PubMed Central

    Nettle, Daniel; Grace, James B.; Choisy, Marc; Cornell, Howard V.; Guégan, Jean-François; Hochberg, Michael E.

    2007-01-01

    Background Social scientists have suggested that cultural diversity in a nation leads to societal instability. However, societal instability may be affected not only by within-nation or α diversity, but also diversity between a nation and its neighbours or β diversity. It is also necessary to distinguish different domains of diversity, namely linguistic, ethnic and religious, and to distinguish between the direct effects of diversity on societal instability, and effects that are mediated by economic conditions. Methodology/Principal Findings We assembled a large cross-national dataset with information on α and β cultural diversity, economic conditions, and indices of societal instability. Structural equation modeling was used to evaluate the direct and indirect effects of cultural diversity on economics and societal stability. Results show that different types and domains of diversity have interacting effects. As previously documented, linguistic α diversity has a negative effect on economic performance, and we show that it is largely through this economic mechanism that it affects societal instability. For β diversity, the higher the linguistic diversity among nations in a region, the less stable the nation. But, religious β diversity has the opposite effect, reducing instability, particularly in the presence of high linguistic diversity. Conclusions Within-nation linguistic diversity is associated with reduced economic performance, which, in turn, increases societal instability. Nations which differ linguistically from their neighbors are also less stable. However, religious diversity between neighboring nations has the opposite effect, decreasing societal instability. PMID:17895970

  10. Culture and biology interplay: An introduction.

    PubMed

    Causadias, José M; Telzer, Eva H; Lee, Richard M

    2017-01-01

    Culture and biology have evolved together, influence each other, and concurrently shape behavior, affect, cognition, and development. This special section highlights 2 major domains of the interplay between culture and biology. The first domain is neurobiology of cultural experiences-how cultural, ethnic, and racial experiences influence limbic systems and neuroendocrine functioning-and the second domain is cultural neuroscience-the connections between cultural processes and brain functioning. We include 3 studies on neurobiology of cultural experiences that examine the associations between racial discrimination and heart rate variability (Hill et al., 2016), economic and sociocultural stressors and cortisol levels (Mendoza, Dmitrieva, Perreira, & Watamura, 2016), and unfair treatment and allostatic load (Ong, Williams, Nwizu, & Gruenewald, 2016). We also include 2 studies on cultural neuroscience that investigate cultural group differences and similarities in beliefs, practices, and neural basis of emotion regulation (Qu & Telzer, 2016), and reflected and direct self-appraisals (Pfeifer et al., 2016). We discuss pending challenges and future directions for this emerging field. (PsycINFO Database Record (c) 2017 APA, all rights reserved).

  11. I am against us? Unpacking cultural differences in ingroup favoritism via dialecticism.

    PubMed

    Ma-Kellams, Christine; Spencer-Rodgers, Julie; Peng, Kaiping

    2011-01-01

    The authors proposed a novel explanation for cultural differences in ingroup favoritism (dialecticism) and tested this hypothesis across cultures/ethnicities, domains, and levels of analysis (explicit vs. implicit, cognitive vs. affective). Dialecticism refers to the cognitive tendency to tolerate contradiction and is more frequently found among East Asian than North American cultures. In Study 1, Chinese were significantly less positive, compared to European Americans, in their explicit judgments of family members. Study 2 investigated ingroup attitudes among Chinese, Latinos, and European Americans. Only Chinese participants showed significant in-group derogation, relative to the other groups, and dialecticism (Dialectical Self Scale) was associated with participants' in group attitudes. Study 3 manipulated dialectical versus linear lay beliefs; participants primed with dialecticism showed more negative, explicit ingroup attitudes. Although ingroup disfavoring tendencies were more prevalent among Chinese across studies, they may be a reflection of one's culturally based lay beliefs rather than deep-rooted negative feelings toward one's ingroup.

  12. Cultural affiliation and the importance of health care attributes. Marketers can develop segmentation strategies for targeted patient groups.

    PubMed

    Dolinsky, A L; Stinerock, R

    1998-01-01

    Culturally based values are known to influence consumer purchase decisions, but little is known about how those values affect health care choices. To rectify that situation and provide health care marketers with a framework for developing culturally based segmentation strategies, the authors undertook an exploratory research project in which Hispanic-, African-, and Anglo-Americans were asked to rate the importance of 16 different health care attributes. Those attributes can be grouped under five categories: quality of physician, quality of nurses and other medical staff, economic issues, access to health care, and nonmedically related experiential aspects. Survey responses identified distinct differences in the importance attached to the various attributes by the three cultural groups. The study also looks at the impact of six demographic and social characteristics on the evaluations made by each cultural group. Those characteristics are educational level, gender, age, health status, marital status, and number of people living in the household.

  13. Cross-Cultural Comparison of Self-Construal and Well-Being between Japan and South Korea: The Role of Self-Focused and Other-Focused Relational Selves

    PubMed Central

    Park, Joonha; Norasakkunkit, Vinai; Kashima, Yoshi

    2017-01-01

    Relational self, along with individual and collective selves, is a fundamental aspect that makes up self-concept. Proposing its two aspects: self-focused relational self (i.e., perceiving the self as the object of other people's referential awareness or intentionality) and other-focused relational self (i.e., perceiving the self as being attuned and empathetically connected to close others), the current study explored the way the four selves affect well-being in Japan and South Korea, the East Asian cultures that have been assumed to be homogeneously collectivistic in previous psychological literature. Japanese and Korean participants rated a set of well-being and self-related scales. There were visible sample differences within culture by collection method (classroom vs. online) in relative degrees of selves and related constructs, possibly associated with generational differences. Other-focused relational self was greater in the Korean classroom sample than the Japanese counterpart, whereas no difference was found between the online samples. On the other hand, it was consistent between cultures that the two types of relational self showed different associations with social anxiety and self-esteem as expected, and that they predicted well-being in different ways. We discuss implications for the generational differences and their interactions with culture and the importance of separating the two aspects of relational self in the study of self and culture. PMID:28928699

  14. Differential Expression of Osteo-Modulatory Molecules in Periodontal Ligament Stem Cells in Response to Modified Titanium Surfaces

    PubMed Central

    Kim, So Yeon; Yoo, Ji-Yeon; Ohe, Joo-Young; Lee, Jung-Woo; Moon, Ji-Hoi; Kwon, Yong-Dae; Heo, Jung Sun

    2014-01-01

    This study assessed differential gene expression of signaling molecules involved in osteogenic differentiation of periodontal ligament stem cells (PDLSCs) subjected to different titanium (Ti) surface types. PDLSCs were cultured on tissue culture polystyrene (TCPS), and four types of Ti discs (PT, SLA, hydrophilic PT (pmodPT), and hydrophilic SLA (modSLA)) with no osteoinductive factor and then osteogenic activity, including alkaline phosphatase (ALP) activity, mRNA expression of runt-related gene 2, osterix, FOSB, FRA1, and protein levels of osteopontin and collagen type IA, were examined. The highest osteogenic activity appeared in PDLSCs cultured on SLA, compared with the TCPS and other Ti surfaces. The role of surface properties in affecting signaling molecules to modulate PDLSC behavior was determined by examining the regulation of Wnt pathways. mRNA expression of the canonical Wnt signaling molecules, Wnt3a and β-catenin, was higher on SLA and modSLA than on smooth surfaces, but gene expression of the calcium-dependent Wnt signaling molecules Wnt5a, calmodulin, and NFATc1 was increased significantly on PT and pmodPT. Moreover, integrin α2/β1, sonic hedgehog, and Notch signaling molecules were affected differently by each surface modification. In conclusion, surface roughness and hydrophilicity can affect differential Wnt pathways and signaling molecules, targeting the osteogenic differentiation of PDLSCs. PMID:25057487

  15. Differential expression of osteo-modulatory molecules in periodontal ligament stem cells in response to modified titanium surfaces.

    PubMed

    Kim, So Yeon; Yoo, Ji-Yeon; Ohe, Joo-Young; Lee, Jung-Woo; Moon, Ji-Hoi; Kwon, Yong-Dae; Heo, Jung Sun

    2014-01-01

    This study assessed differential gene expression of signaling molecules involved in osteogenic differentiation of periodontal ligament stem cells (PDLSCs) subjected to different titanium (Ti) surface types. PDLSCs were cultured on tissue culture polystyrene (TCPS), and four types of Ti discs (PT, SLA, hydrophilic PT (pmodPT), and hydrophilic SLA (modSLA)) with no osteoinductive factor and then osteogenic activity, including alkaline phosphatase (ALP) activity, mRNA expression of runt-related gene 2, osterix, FOSB, FRA1, and protein levels of osteopontin and collagen type IA, were examined. The highest osteogenic activity appeared in PDLSCs cultured on SLA, compared with the TCPS and other Ti surfaces. The role of surface properties in affecting signaling molecules to modulate PDLSC behavior was determined by examining the regulation of Wnt pathways. mRNA expression of the canonical Wnt signaling molecules, Wnt3a and β-catenin, was higher on SLA and modSLA than on smooth surfaces, but gene expression of the calcium-dependent Wnt signaling molecules Wnt5a, calmodulin, and NFATc1 was increased significantly on PT and pmodPT. Moreover, integrin α2/β1, sonic hedgehog, and Notch signaling molecules were affected differently by each surface modification. In conclusion, surface roughness and hydrophilicity can affect differential Wnt pathways and signaling molecules, targeting the osteogenic differentiation of PDLSCs.

  16. Cultural differences in responses to a Likert scale.

    PubMed

    Lee, Jerry W; Jones, Patricia S; Mineyama, Yoshimitsu; Zhang, Xinwei Esther

    2002-08-01

    Cultural differences in responses to a Likert scale were examined. Self-identified Chinese, Japanese, and Americans (N=136, 323, and 160, respectively) recruited at ethnic or general supermarkets in Southern California completed a 13-question Sense of Coherence scale with a choice of either four, five, or seven responses in either Chinese, Japanese, or English. The Japanese respondents more frequently reported difficulty with the scale, the Chinese more frequently skipped questions, and both these groups selected the midpoint more frequently on items that involved admitting to a positive emotion than did the Americans, who were more likely to indicate a positive emotion. Construct validity of the scale tended to be better for the Chinese and the Americans when there were four response choices and for the Japanese when there were seven. Although culture affected response patterns, the association of sense of coherence and health was positive in all three cultural groups. Copyright 2002 Wiley Periodicals, Inc.

  17. Culture shock and travelers.

    PubMed

    Stewart, L; Leggat, P A

    1998-06-01

    As travel has become easier and more affordable, the number of people traveling has risen sharply. People travel for many and varied reasons, from the business person on an overseas assignment to backpackers seeking new and exotic destinations. Others may take up residence in different regions, states or countries for family, business or political reasons. Other people are fleeing religious or political persecution. Wherever they go and for whatever reason they go, people take their culture with them. Culture, like language, is acquired innately in early childhood and is then reinforced through formal and complex informal social education into adulthood. Culture provides a framework for interpersonal and social interactions. Therefore, the contact with a new culture is often not the exciting or pleasurable experience anticipated. When immersed in a different culture, people no longer know how to act when faced with disparate value systems. Contact with the unfamiliar culture can lead to anxiety, stress, mental illness and, in extreme cases, physical illness and suicide. "Culture shock" is a term coined by the anthropologist Oberg. It is the shock of the new. It implies that the experience of the new culture is an unpleasant surprise or shock, partly because it is unexpected and partly because it can lead to a negative evaluation of one's own culture. It is also known as cross-cultural adjustment, being that period of anxiety and confusion experienced when entering a new culture. It affects people intellectually, emotionally, behaviorally and physically and is characterized by symptoms of psychological distress. Culture shock affects both adults and children. In travelers or workers who have prolonged sojourns in foreign countries, culture shock may occur not only as they enter the new culture, but also may occur on their return to their original culture. Children may also experience readjustment problems after returning from leading sheltered lives in expatriate compounds. This readjustment back to their own culture after a period of time abroad has been termed "reverse culture shock, a condition which has been studied in both corporate managers and Peace Corps volunteers. With culture shock and many other processes of psychological adjustment, people tend to suffer alone, thinking that they are the only ones not coping well with their new circumstance. The objective of this paper was to bring the phenomenon of culture shock to the awareness of travel health advisors, who can in turn advise travelers, especially longer term travelers, about having realistic expectations of their travel and life in new cultures.

  18. Using Artificial Orthographies for Studying Cross-Linguistic Differences in the Cognitive and Neural Profiles of Reading

    PubMed Central

    Hirshorn, Elizabeth A.; Fiez, Julie A.

    2017-01-01

    Reading and writing are cultural inventions that have become vital skills to master in modern society. Unfortunately, writing systems are not equally learnable and many individuals struggle to become proficient readers. Languages and their writing systems often have co-varying characteristics, due to both psycholinguistic and socio-cultural forces. This makes it difficult to determine the source of cross-linguistic differences in reading and writing. Nonetheless, it is important to make progress on this issue: a more precise understanding of the factors that affect reading disparities should improve reading instruction theory and practice, and the diagnosis and treatment of reading disorders. In this review, we consider the value of artificial orthographies as a tool for unpacking the factors that create cognitive and neural differences in reading acquisition and skill. We do so by focusing on one dimension that differs among writing systems: grain size. Grain size, or the unit of spoken language that is mapped onto a visual graph, is thought to affect learning, but its impact is still not well understood. We review relevant literature about cross-linguistic writing system differences, the benefits of using artificial orthographies as a research tool, and our recent work with an artificial alphasyllabic writing system for English. We conclude that artificial orthographies can be used to elucidate cross-linguistic principles that affect reading and writing. PMID:28280288

  19. Affect asymmetry and comfort food consumption.

    PubMed

    Dubé, Laurette; LeBel, Jordan L; Lu, Ji

    2005-11-15

    It is proposed that the emotional triggers of comfort food consumption can reliably be predicted by factors tied to affect asymmetry whereby negative affects dominate one's experience, decision making and behaviors in some instances while positive emotions prevail in others. Specifically, we relate three of these factors (age, gender, and culture) to differences in the emotional triggers of comfort food consumption and we further explore the possibility that the type of food eaten during comfort-seeking episodes can also be tied to affect asymmetry. Two hundred and seventy-seven participants completed a web-based survey conducted to assess the emotional antecedents and consequences of comfort food consumption. Consistent with expectations, results indicate that men's comfort food consumption was motivated by positive emotions whereas women's consumption was triggered by negative affects. Consumption of comfort foods alleviated women's negative emotions but also produced guilt. Positive affect was a particularly powerful trigger of comfort food consumption for older participants and for participants with French cultural background. Younger participants and participants with English background reported more intense negative emotions prior to consuming comfort foods. Foods high in sugar and fat content were more efficient in alleviating negative affects whereas low-calorie foods were more efficient in increasing positive emotions.

  20. [Comparison of traditional cardiovascular risk factors frequency among women of different education levels].

    PubMed

    Martínez-Palomino, Guadalupe; Vallejo, Maite; Gárcia-Moreno, Juan; López-Pérez, Maritza; Díaz-Granados, Rosario; Badillo-Castillo, Matilde Osvelia; Garza-Rodarte, Adriana

    2008-01-01

    Ischemic heart disease is the first cause of death in the world in both genders between 30 and 40 years of age. It has been proposed that socioeconomic status could affect the prevalence of cardiovascular risk factors (CVRF), as well as cardiovascular disease incidence and mortality. The purpose of this work was to compare the frequency of CVRF in two groups of women with different educational level. A higher frequency of visceral obesity was identified in the women with lower educational level and hypo-HDL-C in the group of women with higher educational level. Correlation between age and modifiable CVRF was different between the studied groups. A larger proportion of women with higher educational level than those with lower educational level drank alcoholic beverages and smoked cigarettes. Frequency of identified modifiable CVRF was similar to that found in other Hispanic-American populations. The inverse relationship between CVRF and educational level, a commonly used measure of socioeconomic status, and prevalence of CVRF informed in English and American studies was not observed in this investigation; probably because social and cultural conditions could affect the educational level in a different manner. Health education programs must take into account the cultural processes of each country, city, or community, regardless of the socioeconomic status, based on social and cultural backgrounds of each group.

  1. Regional Cultures and the Psychological Geography of Switzerland: Person-Environment-Fit in Personality Predicts Subjective Wellbeing.

    PubMed

    Götz, Friedrich M; Ebert, Tobias; Rentfrow, Peter J

    2018-01-01

    The present study extended traditional nation-based research on person-culture-fit to the regional level. First, we examined the geographical distribution of Big Five personality traits in Switzerland. Across the 26 Swiss cantons, unique patterns were observed for all traits. For Extraversion and Neuroticism clear language divides emerged between the French- and Italian-speaking South-West vs. the German-speaking North-East. Second, multilevel modeling demonstrated that person-environment-fit in Big Five, composed of elevation (i.e., mean differences between individual profile and cantonal profile), scatter (differences in mean variances) and shape (Pearson correlations between individual and cantonal profiles across all traits; Furr, 2008, 2010), predicted the development of subjective wellbeing (i.e., life satisfaction, satisfaction with personal relationships, positive affect, negative affect) over a period of 4 years. Unexpectedly, while the effects of shape were in line with the person-environment-fit hypothesis (better fit predicted higher subjective wellbeing), the effects of scatter showed the opposite pattern, while null findings were observed for elevation. Across a series of robustness checks, the patterns for shape and elevation were consistently replicated. While that was mostly the case for scatter as well, the effects of scatter appeared to be somewhat less robust and more sensitive to the specific way fit was modeled when predicting certain outcomes (negative affect, positive affect). Distinguishing between supplementary and complementary fit may help to reconcile these findings and future research should explore whether and if so under which conditions these concepts may be applicable to the respective facets of person-culture-fit.

  2. The Effects of Affect on Study Abroad Students

    ERIC Educational Resources Information Center

    Savicki, Victor

    2013-01-01

    Being a study abroad student is not all sweetness and light. By definition, study abroad students are faced with acculturative stress (Berry, 2005) by virtue of encountering differences in assumptions, values, and expectations of daily living in their host culture. Add to that the usual challenge of hearing and speaking a different language, and…

  3. Variation in pestivirus growth in testicle primary cell culture is more dependent on the individual cell donor than cattle breed

    USDA-ARS?s Scientific Manuscript database

    Bovine respiratory disease complex (BRDC) affects cattle from all breeds living in all regions. However, different breeds of cattle appear to have different susceptibilities to developing BRDC. The causes of BRDC are multifactorial and include infection with both viral and bacterial pathogens. Infec...

  4. Differing Reactions to Television in Kibbutz and City Children.

    ERIC Educational Resources Information Center

    Huesmann, L. Rowell; Bachrach, Riva S.

    Children's social and cultural environments may affect their perceptions of the reality of television violence. One of the problems in measuring the importance of societal variables is the difficulty in finding children whose social environments have differed for most of their lives in well prescribed ways. An exception to this are kibbutz- and…

  5. Objects of Protection, Enduring Nodes of Difference: Disability Intersections with "Other" Differences, 1916 to 2016

    ERIC Educational Resources Information Center

    Artiles, Alfredo J.; Dorn, Sherman; Bal, Aydin

    2016-01-01

    The purpose of this chapter is to contribute a cultural-historical analytical perspective on disability and its intersections. We assume that disability is socially, historically, and spatially constructed. This standpoint enables us to understand and disrupt disparities in education that affect students living at the intersection of disability…

  6. Formative Assessment Probes: Assessment for All

    ERIC Educational Resources Information Center

    Keeley, Page

    2014-01-01

    Today's elementary teachers are faced with many different types of learners in their classrooms. Students come to the science classroom with a wide range of out-of-school experiences, different cultural and economic backgrounds, languages, learning styles, and cognitive levels that affect how they engage in and learn science. Page Keeley,…

  7. The uptake of different iron salts by the yeast Saccharomyces cerevisiae

    PubMed Central

    Gaensly, Fernanda; Picheth, Geraldo; Brand, Debora; Bonfim, Tania M.B.

    2014-01-01

    Yeasts can be enriched with microelements, including iron; however, special physicochemical conditions are required to formulate a culture media that promotes both yeast growth and iron uptake. Different iron sources do not affect biomass formation; however, considering efficacy, cost, stability, and compatibility with Saccharomyces cerevisiae metabolism, ferrous sulphate is recommended. PMID:25242932

  8. Case Studies in Diversity: Individual Differences in Abilities and Traits of Young Gifted Children.

    ERIC Educational Resources Information Center

    Hafenstein, Norma Lu; Tucker, Brooke

    This study documented how individual differences in personal experiences, cultures, learning styles, and interests affect the demonstrated abilities of children who are gifted, based on qualitative case study research with five children from early childhood classes at the University of Denver's Ricks Center for Gifted Children. Information was…

  9. Cultural psychopathology: uncovering the social world of mental illness.

    PubMed

    López, S R; Guarnaccia, P J

    2000-01-01

    We review cultural psychopathology research since Kleinman's (1988) important review with the goals of updating past reviews, evaluating current conceptualizations and methods, and identifying emerging substantive trends. Conceptual advances are noted, particularly developments in the definition of culture and the examination of both culture-specific and cultural-general processes. The contributions of the Culture and Diagnosis Task Force for DSM-IV and the World Mental Health Report are reviewed and contrasted. Selected research on anxiety, schizophrenia, and childhood disorders is examined, with particular attention given to the study of ataque de nervios, social factors affecting the course of schizophrenia, and cross-national differences in internalizing and externalizing problems in children. Within the last ten years, cultural psychopathology research has become a significant force. Its focus on the social world holds promise to make significant inroads in reducing suffering and improving people's everyday lives.

  10. Language and cultural contacts among Yukatekan Mayans.

    PubMed

    Hofling, Charles A

    2004-01-01

    The Yukatekan branch of the Maya language family, spread across the Yucatán Peninsula of Mexico, northern Guatemala, and Belize, began to diversify approximately 1,000 years ago. Today it has four branches: Mopan Maya, Itzaj Maya, Lakantun Maya and Yukatek Maya proper, which have widely varying language statuses. Lakantun and Itzaj Maya are seriously threatened, while Mopan appears to have a stable or growing population of approximately 10,000 speakers and Yukatek has a very large number of speakers, perhaps 750,000. However, even many Yukateks believe that their language is threatened and that shift to Spanish is underway. During the past millennia there has been a series of contacts involving migration, trade, warfare, and flight among the different branches, as well as with other Mayan languages and with the Spanish. This paper examines a variety of different kinds of contact, and how the different language varieties were involved and affected. One goal of the paper is to better understand how the dynamics of inter-cultural contacts affects language practices resulting in very different language statuses and ideologies.

  11. A Comparison of Educational Systems of Turkey, Malta, Ireland, Spain, Sweden, Portugal, Finland, Greece, Belgium, the Netherlands and Denmark

    ERIC Educational Resources Information Center

    Gokce, Asiye Toker; Celep, Cevat

    2011-01-01

    Managing people requires ongoing living in a harmony and to educate citizens who would support this status. It is not easy to continue the existence of management which can perform different cultures. The different management style is different educational systems. The management style also directly affects the country's educational philosophy.…

  12. Context Influences on the Subjective Experience of Aging: The Impact of Culture and Domains of Functioning.

    PubMed

    O'Brien, Erica L; Hess, Thomas M; Kornadt, Anna E; Rothermund, Klaus; Fung, Helene; Voss, Peggy

    2017-08-01

    Attitudes about aging influence how people feel about their aging and affect psychological and health outcomes in later life. Given cross-cultural variability in such attitudes, the subjective experience of aging (e.g., subjective age [SA]) may also vary, potentially accounting for culture-specific patterns of aging-related outcomes. Our study explored cultural variation in SA and its determinants. American (N = 569), Chinese (N = 492), and German (N = 827) adults aged 30-95 years completed a questionnaire that included instruments measuring basic demographic information, SA, beliefs about thresholds of old age, control over life changes, and age dependency of changes in eight different life domains (i.e., family, work). Analyses revealed consistency across cultures in the domain-specificity of SA, but differences in the amount of shared variance across domains (e.g., Chinese adults exhibited greater homogeneity across domains than did Americans and Germans). Cultural differences were also observed in levels of SA in some domains, which were attenuated by domain-specific beliefs (e.g., control). Interestingly, beliefs about aging accounted for more cultural variation in SA than did sociodemographic factors (e.g., education). Our results demonstrate that subjective perceptions of aging and everyday functioning may be best understood from a perspective focused on context (i.e., culture, life domain). Given its important relation to functioning, examination of cross-cultural differences in the subjective experience of aging may highlight factors that determine variations in aging-related outcomes that then could serve as targets of culture-specific interventions promoting well-being in later life. © The Author 2017. Published by Oxford University Press on behalf of The Gerontological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  13. The Effect of Preoperative Antimicrobial Prophylaxis on Intraoperative Culture Results in Patients with a Suspected or Confirmed Prosthetic Joint Infection: a Systematic Review.

    PubMed

    Wouthuyzen-Bakker, Marjan; Benito, Natividad; Soriano, Alex

    2017-09-01

    Obtaining reliable cultures during revision arthroplasty is important to adequately diagnose and treat a prosthetic joint infection (PJI). The influence of antimicrobial prophylaxis on culture results remains unclear. Since withholding prophylaxis increases the risk for surgical site infections, clarification on this topic is critical. A systematic review was performed with the following research question: in patients who undergo revision surgery of a prosthetic joint, does preoperative antimicrobial prophylaxis affect the culture yield of intraoperative samples in comparison with nonpreoperative antimicrobial prophylaxis? Seven articles were included in the final analysis. In most studies, standard diagnostic culture techniques were used. In patients with a PJI, pooled analysis showed a culture yield of 88% (145/165) in the prophylaxis group versus 95% (344/362) in the nonprophylaxis group ( P = 0.004). Subanalysis of patients with chronic PJIs showed positive cultures in 88% (78/89) versus 91% (52/57), respectively ( P = 0.59). In patients with a suspected chronic infection, a maximum difference of 4% in culture yield between the prophylaxis and nonprophylaxis groups was observed. With the use of standard culture techniques, antimicrobial prophylaxis seems to affect cultures in a minority of patients. Along with the known risk of surgical site infections due to inadequate timing of antimicrobial prophylaxis, we discourage the postponement of prophylaxis until tissue samples are obtained in revision surgery. Future studies are necessary to conclude whether the small percentage of false-negative cultures after prophylaxis can be further reduced with the use of more-sensitive culture techniques, like sonication. Copyright © 2017 American Society for Microbiology.

  14. Adherence of Escherichia coli O157:H7 to epithelial cells in vitro and in pig gut loops is affected by bacterial culture conditions

    PubMed Central

    Yin, Xianhua; Feng, Yanni; Wheatcroft, Roger; Chambers, James; Gong, Joshua; Gyles, Carlton L.

    2011-01-01

    The objectives of this study were to determine the effect of bacterial culture conditions on adherence of enterohemorrhagic Escherichia coli (EHEC) O157:H7 strain 86-24 in vivo to pig enterocytes and to compare the results with adherence in vitro to cultured HEp-2 and IPEC-J2 cells. Growth of O157:H7 in MacConkey broth (MB) resulted in almost no adherence to both HEp-2 and IPEC-J2 cells; prior exposure of the bacteria to pH 2.5 reduced adherence. There was greater adherence by bacteria from static cultures than by those from shaken cultures and by bacteria cultured in brain–heart infusion (BHI) plus NaHCO3 (BHIN) than by bacteria cultured in BHI. In contrast, in pig ileal loops, bacteria cultured in MB adhered well to enterocytes, and prior exposure to pH 2.5 had no effect on adherence. Among several media tested for their effect on bacterial adherence in the pig intestine, MB and BHIN proved to be the best. Bacterial adherence was dose-dependent and was more extensive in the ileum than in the colon. This study demonstrated that there are remarkable differences between culture conditions that promote adherence of an EHEC O157:H7 strain in vitro and in vivo, that culture conditions profoundly affect adherence to epithelial cells in vitro and in vivo, and that pig ileal loops are better suited to adherence studies than are colon loops. PMID:21731177

  15. Issues on Luck Egalitarianism, Responsibility, and Intercultural Healthcare Policies.

    PubMed

    De Hoyos, Adalberto

    2016-04-01

    This article analyzes the criteria for the distribution of healthcare services through different justice theories such as utilitarianism and liberalism, pointing out the problems that arise when providing services to a culturally diverse population. The international epidemiological setting is a favorable one for discussing personal responsibility and luck egalitarianism; however, some provisions have to be made so that healthcare institutions do not treat ethnic, cultural, religious, and linguistic minorities unfairly. The article concludes by proposing that accommodations and culturally sensible attention should be provided when possible, without affecting the equal opportunity of others to access these services.

  16. University students and mental health. Canada, Britain and Singapore.

    PubMed

    Gold, J H

    1979-01-01

    There are many crucial determinants of the individual outcome and benefit of a university education including the stressful interplay of cultural and socioeconomic factors which are of growing importance in the inflationary 1970's. An epidemiological study of university students from 1969-1972 in Canada, 1973-1974 in Britain, and 1975 in Singapore, attempted to identify stresses leading to mental ill-health in these students. Singapore was chosen as an example of a culture bridging the developed Northern and developing Southern nations of the world. Cultural differences affecting the results of the study are discussed as is the role of social change.

  17. Heterogeneous oxygen availability affects the titer and topology but not the fidelity of plasmid DNA produced by Escherichia coli.

    PubMed

    Jaén, Karim E; Sigala, Juan-Carlos; Olivares-Hernández, Roberto; Niehaus, Karsten; Lara, Alvaro R

    2017-07-04

    Dissolved oxygen tension (DOT) is hardly constant and homogenously distributed in a bioreactor, which can have a negative impact in the metabolism and product synthesis. However, the effects of DOT on plasmid DNA (pDNA) production and quality have not been thoroughly investigated. In the present study, the effects of aerobic (DOT ≥30% air sat.), microaerobic (constant DOT = 3% air sat.) and oscillatory DOT (from 0 to 100% air sat.) conditions on pDNA production, quality and host performance were characterized. Microaerobic conditions had little effect on pDNA production, supercoiled fraction and sequence fidelity. By contrast, oscillatory DOT caused a 22% decrease in pDNA production compared with aerobic cultures. Although in aerobic cultures the pDNA supercoiled fraction was 98%, it decreased to 80% under heterogeneous DOT conditions. The different oxygen availabilities had no effect on the fidelity of the produced pDNA. The estimated metabolic fluxes indicated substantial differences at the level of the pentose phosphate pathway and TCA cycle under different conditions. Cyclic changes in fermentative pathway fluxes, as well as fast shifts in the fluxes through cytochromes, were also estimated. Model-based genetic modifications that can potentially improve the process performance are suggested. DOT heterogeneities strongly affected cell performance, pDNA production and topology. This should be considered when operating or scaling-up a bioreactor with deficient mixing. Constant microaerobic conditions affected the bacterial metabolism but not the amount or quality of pDNA. Therefore, pDNA production in microaerobic cultures may be an alternative for bioreactor operation at higher oxygen transfer rates.

  18. Indian sports nicknames/logos: affective difference between American Indian and non-Indian college students.

    PubMed

    LaRocque, Angela R; McDonald, J Douglas; Weatherly, Jeffrey N; Ferraro, F Richard

    2011-01-01

    The use of American Indian (AI) words and images in athletic teams' nicknames, logos, and mascots remains a controversial issue. This study investigated the emotional impact of the University of North Dakota's "Fighting Sioux" nickname/logo on 33 AI and 36 majority culture (MC) students enrolled at the university. Participants completed the Multiple Affect Adjective Checklist-Revised (MAACL-R) before viewing two slide presentations of Fighting Sioux-related images: one neutral (i.e., non-controversial) and one controversial. Participants completed the MAACL-R after each presentation. They also completed the Nickname and Logo Distress Scale, and AI participants completed the Northern Plains Biculturalism Inventory to assess their degree of cultural orientation. Results showed that AIs experienced higher negative affect following both slide presentations than did MC participants. MC participants' affect was only changed following the controversial slide presentation. The findings suggest AI students may experience significantly higher levels of psychological distress when viewing even neutral images of AI nicknames/logos.

  19. Metabolite profiling of microfluidic cell culture conditions for droplet based screening.

    PubMed

    Bjork, Sara M; Sjostrom, Staffan L; Andersson-Svahn, Helene; Joensson, Haakan N

    2015-07-01

    We investigate the impact of droplet culture conditions on cell metabolic state by determining key metabolite concentrations in S. cerevisiae cultures in different microfluidic droplet culture formats. Control of culture conditions is critical for single cell/clone screening in droplets, such as directed evolution of yeast, as cell metabolic state directly affects production yields from cell factories. Here, we analyze glucose, pyruvate, ethanol, and glycerol, central metabolites in yeast glucose dissimilation to establish culture formats for screening of respiring as well as fermenting yeast. Metabolite profiling provides a more nuanced estimate of cell state compared to proliferation studies alone. We show that the choice of droplet incubation format impacts cell proliferation and metabolite production. The standard syringe incubation of droplets exhibited metabolite profiles similar to oxygen limited cultures, whereas the metabolite profiles of cells cultured in the alternative wide tube droplet incubation format resemble those from aerobic culture. Furthermore, we demonstrate retained droplet stability and size in the new better oxygenated droplet incubation format.

  20. mRNA expression pattern of selected candidate genes differs in bovine oviductal epithelial cells in vitro compared with the in vivo state and during cell culture passages.

    PubMed

    Danesh Mesgaran, Sadjad; Sharbati, Jutta; Einspanier, Ralf; Gabler, Christoph

    2016-08-15

    The mammalian oviduct provides the optimal environment for gamete maturation including sperm capacitation, fertilization, and development of the early embryo. Various cell culture models for primary bovine oviductal epithelial cells (BOEC) were established to reveal such physiological events. The aim of this study was to evaluate 17 candidate mRNA expression patterns in oviductal epithelial cells (1) in transition from in vivo cells to in vitro cells; (2) during three consecutive cell culture passages; (3) affected by the impact of LOW or HIGH glucose content media; and (4) influenced by different phases of the estrous cycle in vivo and in vitro. In addition, the release of a metabolite and proteins from BOEC at two distinct cell culture passage numbers was estimated to monitor the functionality. BOEC from 8 animals were isolated and cultured for three consecutive passages. Total RNA was extracted from in vivo and in vitro samples and subjected to reverse transcription quantitative polymerase chain reaction to reveal mRNA expression of selected candidate genes. The release of prostaglandin E2 (PGE2), oviduct-specific glycoprotein 1 (OVGP1) and interleukin 8 (IL8) by BOEC was measured by EIA or ELISA after 24 h. Almost all candidate genes (prostaglandin synthases, enzymes of cellular metabolism and mucins) mRNA expression pattern differed compared in vivo with in vitro state. In addition, transcription of most candidate genes was influenced by the number of cell culture passages. Different glucose medium content did not affect mRNA expression of most candidate genes. The phase of the estrous cycle altered some candidate mRNA expression in BOEC in vitro at later passages. The release of PGE2 and OVGP1 between passages did not differ. However, BOEC in passage 3 released significantly higher amount of IL8 compared with cells in passage 0. This study supports the hypothesis that candidate mRNA expression in BOEC was influenced by transition from the in vivo situation to the new in vitro environment and during consecutive passages. The consequence of cell culture passaging on BOEC ability to release bioactive compounds should be considered.

  1. Rater Evaluations for Psychiatric Instruments and Cultural Differences: The PANSS in China and United States

    PubMed Central

    Aggarwal, Neil Krishan; Zhang, Xiang Yang; Stefanovics, Elina; Chen, Da Chun; Xiu, Mei Hong; Xu, Ke; Rosenheck, Robert A.

    2013-01-01

    This article compares Positive and Negative Syndrome Scale (PANSS) data from Chinese and American inpatients with chronic schizophrenia to show how differences in item ratings may reflect cultural attitudes of raters. The Chinese sample (N=504) came from Beijing Huilongguan Hospital. The American sample came from 268 PANSS assessments of CATIE subjects hospitalized for 15 days or more to optimize equivalence of the samples. Controlling for age and gender, the Chinese sample scored significantly lower for total score by 25% (p<.0001), for the positive sub-scale by 35% (p<.0001), and on the general sub-scale by 32% (p<.0001), but not significantly different on the negative sub-scale score (+0.26%, p=0.76). However, the Chinese sample scored 26% higher on the item on poor rapport (p<.0001), 10.2% higher on passive social withdrawal (p=.003), and most notably 46% higher on the item on lack of judgment and insight (p<.0001). These results remain broadly consistent across gender sub-group analyses. Differences seem to be best explained by both cultural differences in patient clinical presentations as well as varying American and Chinese cultural values affecting rater judgment. PMID:22922237

  2. Refining clinical phenotypes by contrasting ethnically different populations with schizophrenia from Australia, India and Sarawak.

    PubMed

    McLean, Duncan; John, Sujit; Barrett, Robert; McGrath, John; Loa, Peter; Thara, Rangaswamy; Mowry, Bryan

    2012-04-30

    We contrasted demographic and clinical characteristics in transethnic schizophrenia populations from Australia (n=821), India (n=520) and Sarawak, Malaysia (n=298) and proposed cultural explanations for identified site differences. From these we aimed to identify candidate variables free from significant cultural confounding that are hence suitable for inclusion in genetic analyses. We observed five phenomena: (1) more individuals were living alone in Australia than India or Sarawak; (2) drug use was lower in India than Australia or Sarawak; (3) duration of untreated psychosis (DUP) was longer in India than Australia or Sarawak; (4) the rate of schizoaffective disorder was lower in India than Australia or Sarawak; and (5) age at psychosis onset (AAO) was older in Sarawak than Australia or India. We suggest that site differences for living arrangements, drug use and DUP are culturally confounded. The schizoaffective site difference likely results from measurement bias. The AAO site difference, however, has no obvious cultural or measurement bias explanation. Therefore, this may be an ideal candidate for use in genetic studies, given that genetic variants affecting AAO have already been proposed. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

  3. Connecting across Distances and Differences

    ERIC Educational Resources Information Center

    Kippes, Mae Willa

    2004-01-01

    The curriculum continuity for students in isolated rural areas is affected due to the difficulty in getting teachers. Thus, there is a lack of cultural events such as musical performances that hinder students' horizons as a result of this geographical isolation.

  4. Identifying innovation in laboratory studies of cultural evolution: rates of retention and measures of adaptation

    PubMed Central

    Caldwell, Christine A.; Cornish, Hannah; Kandler, Anne

    2016-01-01

    In recent years, laboratory studies of cultural evolution have become increasingly prevalent as a means of identifying and understanding the effects of cultural transmission on the form and functionality of transmitted material. The datasets generated by these studies may provide insights into the conditions encouraging, or inhibiting, high rates of innovation, as well as the effect that this has on measures of adaptive cultural change. Here we review recent experimental studies of cultural evolution with a view to elucidating the role of innovation in generating observed trends. We first consider how tasks are presented to participants, and how the corresponding conceptualization of task success is likely to influence the degree of intent underlying any deviations from perfect reproduction. We then consider the measures of interest used by the researchers to track the changes that occur as a result of transmission, and how these are likely to be affected by differing rates of retention. We conclude that considering studies of cultural evolution from the perspective of innovation provides us with valuable insights that help to clarify important differences in research designs, which have implications for the likely effects of variation in retention rates on measures of cultural adaptation. PMID:26926283

  5. Cultural competence and medical education: many names, many perspectives, one goal.

    PubMed

    Betancourt, Joseph R

    2006-06-01

    Two contemporary reports from the Institute of Medicine--Crossing the Quality Chasm and Unequal Treatment--highlighted the importance of patient-centered care and cultural competence training as a means of improving the quality of health care for all and eliminating racial/ethnic disparities in health care. Previous efforts in cultural competence have aimed to teach about the attitudes, values, beliefs, and behavior of certain groups. A more effective approach is to learn a practical framework to guide inquiry with individual patients about how social, cultural, or economic factors influence their health values, beliefs, and behaviors. Rather than learning about individual cultures and their characteristics, this approach focuses on the issues that arise most commonly due to cultural differences, and how they may affect a physician's interaction with any patient. At the end of the day, physicians need a practical set of tools and skills that will enable them to provide quality care to patients everywhere, from anywhere, with whatever differences in background that may exist, in what is likely to be a brief clinical encounter. Call it what you will, the field of cultural competence aims quite simply to assure that health care providers are prepared to provide quality care to diverse populations.

  6. Understanding Chinese immigrant and European American mothers' expressions of warmth.

    PubMed

    Cheah, Charissa S L; Li, Jin; Zhou, Nan; Yamamoto, Yoko; Leung, Christy Y Y

    2015-12-01

    Maternal warmth, the quality of the affectional bond between mothers and their children, has been found to be consistently associated with children's positive developmental outcomes in Western cultures. However, researchers debate the potential differences in the cultural meanings of maternal warmth, particularly between Chinese and European American families. To address the lack of empirical research on this issue, the present study examined culturally derived perceptions and practices of maternal warmth through open-ended interviews with 70 Chinese immigrant and 70 European American mothers of preschoolers. Specifically, we compared mothers' perceived importance and degree of expression of warmth toward their children, and why and how they express warmth toward their children in the 2 cultural groups. Results showed that, although mothers perceived expressing warmth to be similarly important, European American mothers perceived expressing more warmth toward their children. Moreover, both cultural similarities and differences were found in why these 2 groups of mothers believed it was important to express warmth and the specific practices they used. Chinese immigrant mothers' responses were interpreted as reflecting a cultural emphasis on nurturance and instrumental support, whereas European American mothers' responses reflected the Western cultural focus on more direct and outward demonstrations of warmth. (c) 2015 APA, all rights reserved).

  7. Exploring Emotive Verbs in Persian and English Short Stories: A Contrastive Sociopragmatic Approach.

    PubMed

    Karimi, Keihaneh; Biria, Reza

    2017-04-01

    Current developments in the areas of discourse analysis and cross-cultural studies have led to an increased interest in the way people of different cultures express their affections on various occasions. Individuals learn how to regulate their emotional reactions according to sociocultural norms of behavior defined by the cultures to which they belong. Accordingly, this article aimed to investigate the linguistic expression of emotions in English and Persian short stories in order to fathom out the impact of culture on the way feelings are expressed cross-culturally. For this purpose, a corpus of eight different English and Persian short stories, four in each language, was selected based on a purposive sampling method. Then, using Devon's (The origin of emotions, 2006) typology of emotions, different types of emotive verbs were selected as the unit of analysis. Finally, the frequency and percentage values of emotive verb tokens used in these stories were carefully tabulated in terms of types and their respective metalinguistic categories introduced by Wierzbicka (Emotions across languages and cultures: diversity and universals, Cambridge University Press, Cambridge, 1999). The results obtained from the analysis of the targeted corpora reflected that English and Persian writers employ different types of emotive verbs in expressing their feelings. Essentially, the findings of the present study may have important implications for language teachers, material developers, and course designers.

  8. A cross-cultural longitudinal examination of the effect of cumulative adversity on the mental and physical health of older adults.

    PubMed

    Palgi, Yuval; Shrira, Amit

    2016-03-01

    Self-oriented adversity refers to traumatic events that primarily inflict the self, whereas other-oriented adversity refers to events that affect the self by primarily targeting others. The present study aimed to examine whether cultural background moderates the effects of self-oriented and other-oriented adversity on mental and physical health of older adults. Using longitudinal data from the Israeli component of the Survey of Health and Retirement, we focused on 370 Jews and 239 Arabs who reported their exposure to various adversities across the life span, and completed questionnaires regarding mental and physical health. Results showed that the effect of self-oriented adversity on health did not differ among Jews and Arabs. However, other-oriented adversity showed a stronger effect on Arabs' mental and physical health than on Jews' health. Our findings suggest that the accumulation of adverse events that affect the self by primarily targeting others may have a stronger impact in collectivist cultures than in individualist cultures. (c) 2016 APA, all rights reserved).

  9. The impact of service-learning on cultural competence.

    PubMed

    Amerson, Roxanne

    2010-01-01

    Service-learning provides an excellent pedagogy for introducing students to clients of different cultural backgrounds, helping students become aware of the issues these clients face related to culture and health care, and teaching culturally appropriate care. The Transcultural Self-Efficacy Tool was used to evaluate self-perceived cultural competence in a convenience sample of 60 baccalaureate nursing students enrolled in a community health nursing course following the completion of service-learning projects with local and international communities. Pre- and posttests were analyzed based on total scores and subscale (cognitive, practical, and affective) scores. A paired-samples t test compared the mean pretest total score to the mean posttest total score, which demonstrated a significant increase. In addition, paired-samples t tests demonstrated a significant increase in each subscale.

  10. Cell culture density affects the proliferation activity of human adipose tissue stem cells.

    PubMed

    Kim, Dae Seong; Lee, Myoung Woo; Ko, Young Jong; Chun, Yong Hoon; Kim, Hyung Joon; Sung, Ki Woong; Koo, Hong Hoe; Yoo, Keon Hee

    2016-01-01

    In this study, we investigated the effect of cell density on the proliferation activity of human mesenchymal stem cells (MSCs) derived from adipose tissue (AT-MSCs) over time in culture. Passage #4 (P4) and #12 (P12) AT-MSCs from two donors were plated at a density of 200 (culture condition 1, CC1) or 5000 (culture condition 2, CC2) cells cm(-2) . After 7 days of incubation, P4 and P12 AT-MSCs cultured in CC1 were thin and spindle-shaped, whereas those cultured in CC2 had extensive cell-to-cell contacts and an expanded cell volume. In addition, P4 and P12 AT-MSCs in CC1 divided more than three times, while those in CC2 divided less than once on average. Flow cytometric analysis using 5(6)-carboxyfluorescein diacetate N-succinimidyl ester dye showed that the fluorescence intensity of AT-MSCs was lower in CC1 than in CC2. Furthermore, expression of proliferation-associated genes, such as CDC45L, CDC20A and KIF20A, in P4 AT-MSCs was higher in CC1 than in CC2, and this difference was also observed in P12 AT-MSCs. These data demonstrated that cell culture density affects the proliferation activity of MSCs, suggesting that it is feasible to design a strategy to prepare suitable MSCs using specific culture conditions. Copyright © 2016 John Wiley & Sons, Ltd.

  11. Campylobacter fetus subsp. jejuni in poultry reared under different management systems in Nigeria.

    PubMed

    Adekeye, J O; Abdu, P A; Bawa, E K

    1989-01-01

    Cloacal swabs from 487 live birds in 36 flocks and 70 poultry carcasses were cultured for Campylobacter fetus subsp. jejuni. It was isolated from 12.3% of the birds in 19 flocks. Chickens, turkeys, and guinea fowl differed from one another in isolation rates of the organism. Management system affected its occurrence, and only 7.1% of eviscerated carcasses yielded it. It was concluded that bird species, management system, and immersing slaughtered poultry in boiling water before dressing affect recovery of C. fetus subsp. jejuni from live birds and carcasses.

  12. Examining Cross-Cultural Affective Components of Global Competence from a Value Perspective

    ERIC Educational Resources Information Center

    Awaida-Nachabe, Nadia

    2017-01-01

    The purpose of this study was to explore perceived importance of cultural values and affective components in the Middle East and North Africa (MENA) region and their relationships. This study identified which of the nine affective components of global competence and four higher order cultural values were perceived to be important in the MENA…

  13. Parental attitudes toward adolescent sexuality: transcultural perspectives.

    PubMed

    DeSantis, L; Thomas, J T

    1987-08-01

    The problem of teenage pregnancy continues to impact private and public resources, affecting all socioeconomic and cultural groups. A key factor for nurse practitioners to consider when planning sex education programs is the differing parental attitudes toward teenage sexuality. These attitudes are especially important to keep in mind when dealing with parents from minority cultural groups, as these groups are often highly influential in determining the nature of adolescent sexual behavior and attitudes toward reproduction. A study of Cuban and Haitian child-rearing practices clearly demonstrates two divergent parental views of adolescent sexuality. Nurse practitioners must recognize these differing views, and individualize their approach, in order to develop culturally sensitive sex education programs for adolescents and their parents. Suggestions are provided for development of such programs for Cuban and Haitian parents and children.

  14. Common mental health problems in immigrants and refugees: general approach in primary care

    PubMed Central

    Kirmayer, Laurence J.; Narasiah, Lavanya; Munoz, Marie; Rashid, Meb; Ryder, Andrew G.; Guzder, Jaswant; Hassan, Ghayda; Rousseau, Cécile; Pottie, Kevin

    2011-01-01

    Background: Recognizing and appropriately treating mental health problems among new immigrants and refugees in primary care poses a challenge because of differences in language and culture and because of specific stressors associated with migration and resettlement. We aimed to identify risk factors and strategies in the approach to mental health assessment and to prevention and treatment of common mental health problems for immigrants in primary care. Methods: We searched and compiled literature on prevalence and risk factors for common mental health problems related to migration, the effect of cultural influences on health and illness, and clinical strategies to improve mental health care for immigrants and refugees. Publications were selected on the basis of relevance, use of recent data and quality in consultation with experts in immigrant and refugee mental health. Results: The migration trajectory can be divided into three components: premigration, migration and postmigration resettlement. Each phase is associated with specific risks and exposures. The prevalence of specific types of mental health problems is influenced by the nature of the migration experience, in terms of adversity experienced before, during and after resettlement. Specific challenges in migrant mental health include communication difficulties because of language and cultural differences; the effect of cultural shaping of symptoms and illness behaviour on diagnosis, coping and treatment; differences in family structure and process affecting adaptation, acculturation and intergenerational conflict; and aspects of acceptance by the receiving society that affect employment, social status and integration. These issues can be addressed through specific inquiry, the use of trained interpreters and culture brokers, meetings with families, and consultation with community organizations. Interpretation: Systematic inquiry into patients’ migration trajectory and subsequent follow-up on culturally appropriate indicators of social, vocational and family functioning over time will allow clinicians to recognize problems in adaptation and undertake mental health promotion, disease prevention or treatment interventions in a timely way. PMID:20603342

  15. How do mothers signal shared feeling-states to their infants? An investigation of affect attunement and imitation during the first year of life.

    PubMed

    Jonsson, C O; Clinton, D N; Fahrman, M; Mazzaglia, G; Novak, S; Sörhus, K

    2001-09-01

    The present study examined how mothers signal shared feeling-states to their infants. Affect attunement and imitation were investigated cross-culturally in 39 mother-infant dyads from Sweden (N = 22) and the former Yugoslavia (N = 17) during the first year of life. Video-recordings of playful interaction between mothers and their infants were analysed using the Affect Attunement Protocol. A significant negative association between imitation and age was found, while there was a significant positive association between affect attunement and age. Single occurrences of affect attunement appeared already at two or three months of age, and by 6 months of age episodes of affect attunement were more common than imitation. Frequencies of imitation and affect attunement were similar cross-culturally and in terms of gender, although there was a significant interaction between age and gender. The results suggest that the signalling of shared feeling-states is not a static process. Mothers do not signal shared feeling-states in the same manner at different ages. Imitation is the most important process during the earliest months, but is superseded by affect attunement earlier than previously thought. The functional implications of this developmental variation are discussed.

  16. When cultures clash: a case study of the Texaco takeover of Getty Oil and the impact of acculturation on the acquired firm

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Altendorf, D.M.

    1986-01-01

    Historical surveys of merger and acquisition performance indicate that corporate combinations often do not result in the sought after financial success. An ethnographic case study of the Texaco takeover of Getty Oil was undertaken. Based on the in-depth open-ended interviews, field notes, historical accounts, corporate documents, and organizational symbols, data were analyzed using qualitative techniques explicated by Geertz and Glaser and Strauss. The analysis explored cross cultural contact, employee interpretations resulting in related behavioral and performance outcomes, and the factors influencing the type of acculturation observed. Theoretical properties emerged from descriptive accounts. An integrated theory was developed suggesting that themore » form of acculturation that occurs in the combination of two or more firms is a direct consequence of culture differences and the interpretations that the employees make of these differences. Different occurrences - events, strategies, and activities - contributed to corporate culture clash and organizational change. Results also indicated that how meaning is created and managed affects cross cultural contact, employee assessments, and acculturation. Related propositions were generated and implications for future research discussed.« less

  17. The health of a nation predicts their mate preferences: cross-cultural variation in women's preferences for masculinized male faces

    PubMed Central

    DeBruine, Lisa M.; Jones, Benedict C.; Crawford, John R.; Welling, Lisa L. M.; Little, Anthony C.

    2010-01-01

    Recent formulations of sexual selection theory emphasize how mate choice can be affected by environmental factors, such as predation risk and resource quality. Women vary greatly in the extent to which they prefer male masculinity and this variation is hypothesized to reflect differences in how women resolve the trade-off between the costs (e.g. low investment) and benefits (e.g. healthy offspring) associated with choosing a masculine partner. A strong prediction of this trade-off theory is that women's masculinity preferences will be stronger in cultures where poor health is particularly harmful to survival. We investigated the relationship between women's preferences for male facial masculinity and a health index derived from World Health Organization statistics for mortality rates, life expectancies and the impact of communicable disease. Across 30 countries, masculinity preference increased as health decreased. This relationship was independent of cross-cultural differences in wealth or women's mating strategies. These findings show non-arbitrary cross-cultural differences in facial attractiveness judgements and demonstrate the use of trade-off theory for investigating cross-cultural variation in women's mate preferences. PMID:20236978

  18. Phosphorus limitation and heat stress decrease calcification in Emiliania huxleyi

    NASA Astrophysics Data System (ADS)

    Gerecht, Andrea C.; Šupraha, Luka; Langer, Gerald; Henderiks, Jorijntje

    2018-02-01

    Calcifying haptophytes (coccolithophores) sequester carbon in the form of organic and inorganic cellular components (coccoliths). We examined the effect of phosphorus (P) limitation and heat stress on particulate organic and inorganic carbon (calcite) production in the coccolithophore Emiliania huxleyi. Both environmental stressors are related to rising CO2 levels and affect carbon production in marine microalgae, which in turn impacts biogeochemical cycling. Using semi-continuous cultures, we show that P limitation and heat stress decrease the calcification rate in E. huxleyi. However, using batch cultures, we show that different culturing approaches (batch versus semi-continuous) induce different physiologies. This affects the ratio of particulate inorganic (PIC) to organic carbon (POC) and complicates general predictions on the effect of P limitation on the PIC  /  POC ratio. We found heat stress to increase P requirements in E. huxleyi, possibly leading to lower standing stocks in a warmer ocean, especially if this is linked to lower nutrient input. In summary, the predicted rise in global temperature and resulting decrease in nutrient availability may decrease CO2 sequestration by E. huxleyi through lower overall carbon production. Additionally, the export of carbon may be diminished by a decrease in calcification and a weaker coccolith ballasting effect.

  19. Home-school learning of science: The culture of homes, and pupils' difficult border crossing

    NASA Astrophysics Data System (ADS)

    Solomon, Joan

    2003-02-01

    A British project that explored the way parents and their children of elementary school age carried out simple science activities at home is described and illustrated. Previous research in this field has yielded ambiguous results when evaluated in terms of school science knowledge gained. The basis of the analysis carried out here is largely descriptive using some sociological theory to understand activities in the home. It is argued that home is a special place not only rich in supportive emotions, but also imbued with idiosyncratic attitudes toward science education, which often match with attitudes toward other matters. Schools create different and more uniform cultures for the same children. There has been a long history of calls for collaboration between the two constituencies; however, this article demonstrates that a number of these differences exist which cannot fail to affect children's learning in each situation. Extracts from the children's conversations with their parents during the investigations as well as parents' interpretations of what they are doing will be presented. These vignettes illustrate a wide variation in attitude which affects the children as they daily cross boundaries from one culture to another, trying to preserve what is precious in their home culture. At home the children's participation becomes far more relaxed and personal, just as discussion with their parents is more fluent than at school.

  20. Interactive effects of culture and sex hormones on the sex role self-concept

    PubMed Central

    Pletzer, Belinda; Petasis, Ourania; Ortner, Tuulia M.; Cahill, Larry

    2015-01-01

    Sex role orientation, i.e., a person's masculinity or femininity, influences cognitive and emotional performance, like biological sex. While it is now widely accepted that sex differences are modulated by the hormonal status of female participants (menstrual cycle, hormonal contraceptive use), the question, whether hormonal status and sex hormones also modulate participants sex role orientation has hardly been addressed previously. The present study assessed sex role orientation and hormonal status as well as sex hormone levels in three samples of participants from two different cultures (Northern American, Middle European). Menstrual cycle phase did not affect participant's masculinity or femininity, but had a significant impact on reference group. While women in their follicular phase (low levels of female sex hormones) determined their masculinity and femininity in reference to men, women in their luteal phase (high levels of female sex hormones) determined their masculinity and femininity in reference to women. Hormonal contraceptive users rated themselves as significantly more feminine and less masculine than naturally cycling women. Furthermore, the impact of biological sex on the factorial structure of sex role orientation as well as the relationship of estrogen to masculinity/femininity was modulated by culture. We conclude that culture and sex hormones interactively affect sex role orientation and hormonal status of participants should be controlled for when assessing masculinity and/or femininity. PMID:26236181

  1. Insights into an Optimization of Plasmodium vivax Sal-1 In Vitro Culture: The Aotus Primate Model.

    PubMed

    Shaw-Saliba, Kathryn; Thomson-Luque, Richard; Obaldía, Nicanor; Nuñez, Marlon; Dutary, Sahir; Lim, Caeul; Barnes, Samantha; Kocken, Clemens H M; Duraisingh, Manoj T; Adams, John H; Pasini, Erica M

    2016-07-01

    Malaria is one of the most significant tropical diseases, and of the Plasmodium species that cause human malaria, P. vivax is the most geographically widespread. However, P. vivax remains a relatively neglected human parasite since research is typically limited to laboratories with direct access to parasite isolates from endemic field settings or from non-human primate models. This restricted research capacity is in large part due to the lack of a continuous P. vivax in vitro culture system, which has hampered the ability for experimental research needed to gain biological knowledge and develop new therapies. Consequently, efforts to establish a long-term P. vivax culture system are confounded by our poor knowledge of the preferred host cell and essential nutrients needed for in vitro propagation. Reliance on very heterogeneous P. vivax field isolates makes it difficult to benchmark parasite characteristics and further complicates development of a robust and reliable culture method. In an effort to eliminate parasite variability as a complication, we used a well-defined Aotus-adapted P. vivax Sal-1 strain to empirically evaluate different short-term in vitro culture conditions and compare them with previous reported attempts at P. vivax in vitro culture Most importantly, we suggest that reticulocyte enrichment methods affect invasion efficiency and we identify stabilized forms of nutrients that appear beneficial for parasite growth, indicating that P. vivax may be extremely sensitive to waste products. Leuko-depletion methods did not significantly affect parasite development. Formatting changes such as shaking and static cultures did not seem to have a major impact while; in contrast, the starting haematocrit affected both parasite invasion and growth. These results support the continued use of Aotus-adapted Sal-1 for development of P. vivax laboratory methods; however, further experiments are needed to optimize culture conditions to support long-term parasite development.

  2. Insights into an Optimization of Plasmodium vivax Sal-1 In Vitro Culture: The Aotus Primate Model

    PubMed Central

    Obaldía, Nicanor; Nuñez, Marlon; Dutary, Sahir; Lim, Caeul; Barnes, Samantha; Kocken, Clemens H. M.; Duraisingh, Manoj T.; Adams, John H.; Pasini, Erica M.

    2016-01-01

    Malaria is one of the most significant tropical diseases, and of the Plasmodium species that cause human malaria, P. vivax is the most geographically widespread. However, P. vivax remains a relatively neglected human parasite since research is typically limited to laboratories with direct access to parasite isolates from endemic field settings or from non-human primate models. This restricted research capacity is in large part due to the lack of a continuous P. vivax in vitro culture system, which has hampered the ability for experimental research needed to gain biological knowledge and develop new therapies. Consequently, efforts to establish a long-term P. vivax culture system are confounded by our poor knowledge of the preferred host cell and essential nutrients needed for in vitro propagation. Reliance on very heterogeneous P. vivax field isolates makes it difficult to benchmark parasite characteristics and further complicates development of a robust and reliable culture method. In an effort to eliminate parasite variability as a complication, we used a well-defined Aotus-adapted P. vivax Sal-1 strain to empirically evaluate different short-term in vitro culture conditions and compare them with previous reported attempts at P. vivax in vitro culture Most importantly, we suggest that reticulocyte enrichment methods affect invasion efficiency and we identify stabilized forms of nutrients that appear beneficial for parasite growth, indicating that P. vivax may be extremely sensitive to waste products. Leuko-depletion methods did not significantly affect parasite development. Formatting changes such as shaking and static cultures did not seem to have a major impact while; in contrast, the starting haematocrit affected both parasite invasion and growth. These results support the continued use of Aotus-adapted Sal-1 for development of P. vivax laboratory methods; however, further experiments are needed to optimize culture conditions to support long-term parasite development. PMID:27463518

  3. Cell lineage allocation in equine blastocysts produced in vitro under varying glucose concentrations.

    PubMed

    Choi, Young-Ho; Ross, Pablo; Velez, Isabel C; Macías-García, B; Riera, Fernando L; Hinrichs, Katrin

    2015-07-01

    Equine embryos develop in vitro in the presence of high glucose concentrations, but little is known about their requirements for development. We evaluated the effect of glucose concentrations in medium on blastocyst development after ICSI. In experiment 1, there were no significant differences in rates of blastocyst formation among embryos cultured in our standard medium (DMEM/F-12), which contained >16 mM glucose, and those cultured in a minimal-glucose embryo culture medium (<1 mM; Global medium, GB), with either 0 added glucose for the first 5 days, then 20 mM (0-20) or 20 mM for the entire culture period (20-20). In experiment 2, there were no significant differences in the rates of blastocyst development (31-46%) for embryos cultured in four glucose treatments in GB (0-10, 0-20, 5-10, or 5-20). Blastocysts were evaluated by immunofluorescence for lineage-specific markers. All cells stained positively for POU5F1. An inner cluster of cells was identified that included presumptive primitive endoderm cells (GATA6-positive) and presumptive epiblast (EPI) cells. The 5-20 treatment resulted in a significantly lower number of presumptive EPI-lineage cells than the 0-20 treatment did. GATA6-positive cells appeared to be allocated to the primitive endoderm independent of the formation of an inner cell mass, as was previously hypothesized for equine embryos. These data demonstrate that equine blastocyst development is not dependent on high glucose concentrations during early culture; rather, environmental glucose may affect cell allocation. They also present the first analysis of cell lineage allocation in in vitro-fertilized equine blastocysts. These findings expand our understanding of the factors that affect embryo development in the horse. © 2015 Society for Reproduction and Fertility.

  4. Presepsin (Scd14) as a Marker of Serious Bacterial Infections in Chemotherapy Induced Severe Neutropenia

    PubMed Central

    Olad, Elham; Sedighi, Iraj; Mehrvar, Azim; Tashvighi, Maryam; Fallahazad, Vahid; Hedayatiasl, Amirabbas; Esfahani, Hossein

    2014-01-01

    Objective: Timely detection of serious bacterial infections or prediction of sepsis and death is of paramount importance in neutropenic patients especially in oncology settings. The aim of this study was to determine a rapid and secure predictor of sepsis in severe neutropenic cancer children. Methods: In addition to blood culture, we have evaluated serum soluble CD14 on this role and measured it in 39 neutropenic episodes in Mahak pediatric oncology center from September 2012 to January 2013. Fifteen episodes had positive bacterial cultures and 18 had fever. The mean sCD14 values were compared in the presence or absence of fever, positive blood culture and other clinical conditions. Also, mean levels compared in different white cell counts and different four combination settings of fever and blood culture. Findings: It was statistically higher in febrile episodes, in the presence of oral mucositis, indwelling catheter infection, otitis media, and post toxic epidermal necrolysis sepsis and in instances of death within 15 days. Leukocyte count did not affect sCD14 level and in combinations of fever and blood culture, mean sCD14 values were ranked as follow: febrile culture negatives, febrile culture positives, afebrile culture positives and afebrile culture negatives. Conclusion: Although sCD14 was not sensitive in detection of bacteremia, in the absence of clinically detectable source of infection, it was significantly higher in culture positives. PMID:26019777

  5. Heritability of regeneration in tissue cultures of sweet potato (Ipomoea batatas L.).

    PubMed

    Templeton-Somers, K M; Collins, W W

    1986-03-01

    A population of open-pollinated progeny from 12 parents, and the 12 parents, was surveyed for in vitro growth and regeneration characteristics. Four different tissue culture procedures involving different media and the use of different explants to initiate the cultures were used. Petiole explants from young leaves were used as explants for initiation of callus cultures. These were evaluated for callus growth rate, friability, and callus color and texture, before transferring to each of three different regeneration media for evaluation of morphogenetic potential. Small shoot tips also were used to initiate callus cultures, which were evaluated for the same growth characteristics and transferred to growth-regulator free regeneration media. Regeneration occurred through root or shoot regeneration or through embryogenesis. Tissue culture treatment effects, as well as genotypic effects, were highly significant in determining: the types of callus produced, callus growth rates, color and texture on the two types of media used for the second and third subcultures. The family x treatment interaction was generally not statistically significant, affecting only callus color. Estimates of narrow sense heritability for callus growth rate in both the second and third subcultures were high enough (0.35 and 0.63, respectively) for the evaluation of parental lines for selection procedures. These characteristics were also the only early culture callus traits that were consistently correlated with later morphogenesis of the cultures. They were negatively correlated with root or shoot regeneration. The occurence of somatic embryogenesis was not correlated with early callus growth characteristics. Genetic and treatment effects were highly significant in the evaluation of morphogenetic potential, through root or shoot regeneration, or through embryogenesis. Regeneration of all types was of low frequency for all procedures, expressed in ≦ 11% of the cultures of the total population.

  6. The Associations of Dyadic Coping and Relationship Satisfaction Vary between and within Nations: A 35-Nation Study

    PubMed Central

    Hilpert, Peter; Randall, Ashley K.; Sorokowski, Piotr; Atkins, David C.; Sorokowska, Agnieszka; Ahmadi, Khodabakhsh; Alghraibeh, Ahmad M.; Aryeetey, Richmond; Bertoni, Anna; Bettache, Karim; Błażejewska, Marta; Bodenmann, Guy; Borders, Jessica; Bortolini, Tiago S.; Butovskaya, Marina; Castro, Felipe N.; Cetinkaya, Hakan; Cunha, Diana; David, Oana A.; DeLongis, Anita; Dileym, Fahd A.; Domínguez Espinosa, Alejandra D. C.; Donato, Silvia; Dronova, Daria; Dural, Seda; Fisher, Maryanne; Frackowiak, Tomasz; Gulbetekin, Evrim; Hamamcıoğlu Akkaya, Aslıhan; Hansen, Karolina; Hattori, Wallisen T.; Hromatko, Ivana; Iafrate, Raffaella; James, Bawo O.; Jiang, Feng; Kimamo, Charles O.; King, David B.; Koç, Fırat; Laar, Amos; Lopes, Fívia De Araújo; Martinez, Rocio; Mesko, Norbert; Molodovskaya, Natalya; Moradi, Khadijeh; Motahari, Zahrasadat; Natividade, Jean C.; Ntayi, Joseph; Ojedokun, Oluyinka; Omar-Fauzee, Mohd S. B.; Onyishi, Ike E.; Özener, Barış; Paluszak, Anna; Portugal, Alda; Relvas, Ana P.; Rizwan, Muhammad; Salkičević, Svjetlana; Sarmány-Schuller, Ivan; Stamkou, Eftychia; Stoyanova, Stanislava; Šukolová, Denisa; Sutresna, Nina; Tadinac, Meri; Teras, Andero; Tinoco Ponciano, Edna L.; Tripathi, Ritu; Tripathi, Nachiketa; Tripathi, Mamta; Vilchinsky, Noa; Xu, Feng; Yamamoto, Maria E.; Yoo, Gyesook

    2016-01-01

    Objective: Theories about how couples help each other to cope with stress, such as the systemic transactional model of dyadic coping, suggest that the cultural context in which couples live influences how their coping behavior affects their relationship satisfaction. In contrast to the theoretical assumptions, a recent meta-analysis provides evidence that neither culture, nor gender, influences the association between dyadic coping and relationship satisfaction, at least based on their samples of couples living in North America and West Europe. Thus, it is an open questions whether the theoretical assumptions of cultural influences are false or whether cultural influences on couple behavior just occur in cultures outside of the Western world. Method: In order to examine the cultural influence, using a sample of married individuals (N = 7973) from 35 nations, we used multilevel modeling to test whether the positive association between dyadic coping and relationship satisfaction varies across nations and whether gender might moderate the association. Results: Results reveal that the association between dyadic coping and relationship satisfaction varies between nations. In addition, results show that in some nations the association is higher for men and in other nations it is higher for women. Conclusions: Cultural and gender differences across the globe influence how couples' coping behavior affects relationship outcomes. This crucial finding indicates that couple relationship education programs and interventions need to be culturally adapted, as skill trainings such as dyadic coping lead to differential effects on relationship satisfaction based on the culture in which couples live. PMID:27551269

  7. Chondrocyte response to cyclic hydrostatic pressure in alginate versus pellet culture.

    PubMed

    Elder, Steven H; Sanders, Shawn W; McCulley, William R; Marr, Misti L; Shim, Joon W; Hasty, Karen A

    2006-04-01

    Cells are often cultured at high density (e.g., confluent monolayer and as pellets) to promote chondrogenic differentiation and to maintain the chondrocyte phenotype. They are also frequently suspended in hydrogels such as agarose or alginate for the same purposes. These culture techniques differ markedly with respect to frequency of direct contact between cells and overall intercellular spacing. Because these factors may significantly affect mechanotransduction, the purpose of this study was to determine if the response of articular chondrocytes to cyclic hydrostatic pressure would depend on the culture condition. Primary articular chondrocytes from young and mature pigs were cultured either as pellets or suspended in alginate beads. Both groups were exposed to dynamic hydrostatic pressure (4 MPa, 1 Hz, 5400 cycles per day) for 7 days. Cell proliferation was unaffected by pressure, but pressurized chondrocytes in pellet culture had significantly greater sGAG content and incorporated [3H]proline at a higher rate than nonpressurized controls. Electron microscopy revealed a fibrous extracellular matrix (ECM) surrounding pellets, but not cells in alginate. In addition, expression of Connexin 43 (Cx43) mRNA was slightly lower in alginate than in pellet cultures and was not significantly altered by loading. Thus, metabolic response of chondrocytes to dynamic hydrostatic pressure was affected by culture technique; chondrocytes cultured as pellets exhibited the classical anabolic response to dynamic hydrostatic pressure, but those in alginate did not. Although cell-ECM interaction could be important, the differential response is not likely attributable to differential expression of Cx43 mRNA. Copyright 2006 Orthopaedic Research Society

  8. The Diabetes Disparity and Puerto Rican Identified Individuals.

    PubMed

    Johnson, Jalil A; Cavanagh, Stephen; Jacelon, Cynthia S; Chasan-Taber, Lisa

    2017-04-01

    Purpose The purpose of this systematic review was to describe what is known about the diabetes disparity affecting Puerto Rican identified adults living in the continental United States as well as illuminate areas that merit further investigation. Methods The CINAHL and PubMed databases were searched using the keywords Hispanic, Puerto Rican, and type 2 diabetes. Search limits included < 10-year-old, peer-reviewed, systematic reviews, available in the English language. The abstracts of 124 articles were reviewed, and 7 articles were reviewed in depth. Results The Puerto Rican identified Hispanic subgroup is disproportionately affected by diabetes-the diabetes disparity. Puerto Rican identified Hispanic adults are less affected by citizenship status, may be less affected by English proficiency, use health care services differently, and have contextually different fatalistic views of diabetes compared with other Hispanic identified people. Spiritual/religious influences, associated mental health problems, and general cultural practices related to diabetes self-care are understudied in this group. Conclusion Ambiguous use of the term Hispanic should be avoided when describing Hispanic subgroups. Stronger, more robust studies are needed to understand the unique cultural forces influencing the poor diabetes outcomes and individual behaviors that contribute to generally suboptimal diabetes self-care for Puerto Rican adults with type 2 diabetes.

  9. Explanatory models of diabetes in the U.S. and Mexico: the patient-provider gap and cultural competence.

    PubMed

    Weller, Susan C; Baer, Roberta D; Garcia de Alba Garcia, Javier; Salcedo Rocha, Ana L

    2012-09-01

    Successful management of type 2 diabetes requires support and collaboration between diabetic patients, their health care providers, family and community. Using data collected in 1994-2001, we describe illness beliefs of physicians, patients, and representative samples of community members in the US and Mexico. We test whether differences in conceptualizations of diabetes are greater across national and linguistic boundaries or between physicians and lay groups. Interviews were conducted in southern Texas on the Mexican border and in Guadalajara, Mexico. Culturally appropriate interview materials were developed with a mixed-methods approach. Qualitative interviews elicited beliefs about causes, risks, symptoms, and treatments for diabetes and salient themes were incorporated into structured interviews. A cultural consensus analysis was used to verify salient themes within each of the six samples. The consistency in responses in each of the six samples indicated a shared core of beliefs that transcended individual variations. The greatest differences occurred between physician and lay samples; patient and community models were more similar to one another than to the physician models. Differences between physicians and patients may affect optimal management of diabetes, but these differences do not appear to be simply a function of differences in national culture and language, as the largest differences occurred in Mexico. This suggests that rather than cultural competence per se, formal educational levels and class differences may also play an important role in patient understanding and the gap in patient-provider understanding. Copyright © 2012 Elsevier Ltd. All rights reserved.

  10. The "Simmie" Side of Life: Old Order Amish Youths' Affective Response to Culturally Prescribed Deviance.

    ERIC Educational Resources Information Center

    Reiling, Denise M.

    2002-01-01

    Analyzed the counterintuitive affective response Old Order Amish youth make to unique cultural prescriptions for adolescent deviance (constructed by adult Amish culture). Interview data supported the basic principles of Terror Management Theory in an unexpected, indirect fashion. Rather than functioning as a specialized cultural-anxiety buffer…

  11. Ovalocytic erythrocytes from Melanesians are resistant to invasion by malaria parasites in culture.

    PubMed Central

    Kidson, C; Lamont, G; Saul, A; Nurse, G T

    1981-01-01

    Ovalocytic erythrocytes from Melanesians in Papua New Guinea have been demonstrated to be resistant to infection by malaria parasites (Plasmodium falciparum) in culture by using a double-label fluorescence assay of merozoite invasion. That merozoites do not bind irreversibly to ovalocytes has been demonstrated by an assay that measures competition between ovalocytes and normocytes. Analysis of behavior on thermal deformation has demonstrated that ovalocytes are more more thermostable than normocytes, suggesting that there is a major difference in cytoskeletal structure. These findings with P. falciparum and epidemiological data demonstrating clinical resistance to P. vivax and P. malariae suggest that the membrane alterations(s) in these ovalocytes affect(s) invasion step(s) common to all three species of malaria parasite. Images PMID:7029547

  12. Brains, language and the argumentative mind in Western and Eastern societies. The fertile differences between Western-Eastern argumentative traditions.

    PubMed

    Vallverdú, Jordi

    2017-12-01

    The philosophical differences between Western and Eastern philosophy not only derive from general cultural ideas about reality, but as Nisbet writes (2003), are also methodological, ontological, and cognitively driven. Thus, we can see that strategies of thought and theory-generation are constrained and enabled by conceptual levels, and that the existence of differences and within these levels may be pragmatically combined in fruitful ways. At this point, I remark that there is not a single way to connect biology and culture, but at least we need to admit that brains allow the existence of minds and that these create languages, which also organize the world symbolically following a long set of (sometimes interconnected) heuristics. Throughout the paper we will see how fundamental, geographically located cultural perspectives have affected reasoning strategies and discourses, determining the main Western and Eastern Traditions. At the same time, we can conclude that different traditional perspectives allow more diversity for knowledge acquisition. Copyright © 2017 Elsevier Ltd. All rights reserved.

  13. Recreation safety in municipal parks - Bloomington, Indiana and Tsukuba, Japan: a comparison study of risk management

    Treesearch

    Bruce Hronek

    2001-01-01

    Cultural differences affects the design and use of playground equipment in parks. Comparative research exploring the differences in playground facilities, parental supervision, and use was conducted on-site in Tsukuba City, Ibaraki Perfecture, Japan and Bloomington, Indiana, USA. The study examines park design, play equipment, sports fields, use patterns, and parental...

  14. Do English and Mandarin Speakers Think about Time Differently?

    ERIC Educational Resources Information Center

    Boroditsky, Lera; Fuhrman, Orly; McCormick, Kelly

    2011-01-01

    Time is a fundamental domain of experience. In this paper we ask whether aspects of language and culture affect how people think about this domain. Specifically, we consider whether English and Mandarin speakers think about time differently. We review all of the available evidence both for and against this hypothesis, and report new data that…

  15. "You Don't Read a Science Book, You Study It": An Exploration of Cultural Concepts of Reading.

    ERIC Educational Resources Information Center

    Anderson, Jim; Gunderson, Lee

    2001-01-01

    Discusses how the differing views held by teachers and immigrant parents and their children affect early reading instruction, secondary content reading, and reading involving technology. Demonstrates that immigrant students and their parents hold different beliefs about reading and schooling than those held by many teachers. Concludes it is…

  16. Cross cultural differences in unconscious knowledge.

    PubMed

    Kiyokawa, Sachiko; Dienes, Zoltán; Tanaka, Daisuke; Yamada, Ayumi; Crowe, Louise

    2012-07-01

    Previous studies have indicated cross cultural differences in conscious processes, such that Asians have a global preference and Westerners a more analytical one. We investigated whether these biases also apply to unconscious knowledge. In Experiment 1, Japanese and UK participants memorized strings of large (global) letters made out of small (local) letters. The strings constituted one sequence of letters at a global level and a different sequence at a local level. Implicit learning occurred at the global and not the local level for the Japanese but equally at both levels for the English. In Experiment 2, the Japanese preference for global over local processing persisted even when structure existed only at the local but not global level. In Experiment 3, Japanese and UK participants were asked to attend to just one of the levels, global or local. Now the cultural groups performed similarly, indicating that the bias largely reflects preference rather than ability (although the data left room for residual ability differences). In Experiment 4, the greater global advantage of Japanese rather English was confirmed for strings made of Japanese kana rather than Roman letters. That is, the cultural difference is not due to familiarity of the sequence elements. In sum, we show for the first time that cultural biases strongly affect the type of unconscious knowledge people acquire. Copyright © 2012 Elsevier B.V. All rights reserved.

  17. Temperature during conidiation affects stress tolerance, pigmentation, and trypacidin accumulation in the conidia of the airborne pathogen Aspergillus fumigatus

    PubMed Central

    Sakai, Kanae; Suzuki, Satoshi; Umemura, Myco; Nogawa, Toshihiko; Kato, Naoki; Osada, Hiroyuki; Watanabe, Akira; Kawamoto, Susumu; Gonoi, Tohru; Kamei, Katsuhiko

    2017-01-01

    Asexual spores (conidia) are reproductive structures that play a crucial role in fungal distribution and survival. As fungal conidia are, in most cases, etiological agents of plant diseases and fungal lung disease, their stress resistance and interaction with their hosts have drawn increasing attention. In the present study, we investigated whether environmental temperature during conidiation affects the stress tolerance of the conidia of the human pathogenic fungus Aspergillus fumigatus. Conidia from a 25°C culture showed a lower tolerance to heat (60°C) and oxidative (H2O2) stresses and a marked resistance to ultraviolet radiation exposure, compared with those produced at 37 and 45°C. The accumulation of trehalose was lower in the conidia from the 25°C culture. Furthermore, the conidia from the 25°C culture showed darker pigmentation and increased transcripts of dihydroxynaphthalene (DHN)-melanin biosynthesis-related genes (i.e., pksP, arp1, and arp2). An RNA-sequencing analysis revealed that the transcription level of the trypacidin (tpc) gene cluster, which contains 13 genes, was sharply and coordinately activated in the conidia from the 25°C culture. Accordingly, trypacidin was abundant in the conidia from the 25°C culture, whereas there was little trypacidin in the conidia from the 37°C culture. Taken together, these data show that the environmental temperature during conidiation affects conidial properties such as stress tolerance, pigmentation, and mycotoxin accumulation. To enhance our knowledge, we further explored the temperature-dependent production of DHN-melanin and trypacidin in clinical A. fumigatus isolates. Some of the isolates showed temperature-independent production of DHN-melanin and/or trypacidin, indicating that the conidia-associated secondary metabolisms differed among the isolates. PMID:28486558

  18. Configural and scalar invariance of the center for epidemiologic studies depression scale in Egypt and Canada: Differential symptom emphasis across cultures and genders.

    PubMed

    Huang, Vivian; Beshai, Shadi; Korol, Stephanie; Nicholas Carleton, R

    2017-04-01

    Depression is a significant contributor of global disease burden. Previous studies have revealed cross-cultural and gender differences in the presentation of depressive symptoms. Using the Center for Epidemiologic Studies-Depression Scale (CES-D), the present study examined differences in self-reported somatic, negative affective, and anhedonia symptoms of depression among Egyptian and Canadian university students. A total of 338 university students completed study questionnaires from two major universities in Egypt (n=152) and Canada (n=186). Symptom domains were calculated based on the 14-item model of the CES-D. We found significant culture by gender interactions of total CES-D scores, wherein Egyptian females reported higher scores compared to their Canadian and Egyptian male counterparts. Limitations include using analogue student samples and using only one self-report measure to examine different depressive symptom domains. Findings of this study provided support that males and females may differentially report depressive symptoms across cultures. Implications of these results are further discussed. Copyright © 2017 Elsevier Ireland Ltd. All rights reserved.

  19. Japanese-American confinement and scientific democracy: Colonialism, social engineering, and government administration.

    PubMed

    Rosemblatt, Karin Alejandra; Benmergui, Leandro Daniel

    2018-03-01

    During World War II, the U.S. Indian Service conducted social science experiments regarding governance among Japanese Americans imprisoned at the Poston, Arizona, camp. Researchers used an array of techniques culled from anthropological culture and personality studies, psychiatry, psychology, medicine, and public opinion research to probe how the personality traits of the confined Japanese-Americans and camp leaders affected the social interactions within each group and between them. The research drew on prior studies of Indian personality in the US Southwest, Mexico's Native policies, and indirect colonial rule. Researchers asked how democracy functioned in contexts marked by hierarchy and difference. Their goal was to guide future policies toward US "minorities" and foreign races in post-war occupied territories. We show how researchers deployed ideas about race, cultural, and difference across a variety of cases to create a universal, predictive social science, which they combined with a prewar romanticism and cultural relativism. These researchers made ethnic, racial, and cultural difference compatible with predictive laws of science based on notions of fundamental human similarities. © 2018 Wiley Periodicals, Inc.

  20. Culture, emotion regulation, and adjustment.

    PubMed

    Matsumoto, David; Yoo, Seung Hee; Nakagawa, Sanae

    2008-06-01

    This article reports differences across 23 countries on 2 processes of emotion regulation--reappraisal and suppression. Cultural dimensions were correlated with country means on both and the relationship between them. Cultures that emphasized the maintenance of social order--that is, those that were long-term oriented and valued embeddedness and hierarchy--tended to have higher scores on suppression, and reappraisal and suppression tended to be positively correlated. In contrast, cultures that minimized the maintenance of social order and valued individual Affective Autonomy and Egalitarianism tended to have lower scores on Suppression, and Reappraisal and Suppression tended to be negatively correlated. Moreover, country-level emotion regulation was significantly correlated with country-level indices of both positive and negative adjustment. (PsycINFO Database Record (c) 2008 APA, all rights reserved).

  1. NanR Regulates nanI Sialidase Expression by Clostridium perfringens F4969, a Human Enteropathogenic Strain.

    PubMed

    Li, Jihong; Evans, Daniel R; Freedman, John C; McClane, Bruce A

    2017-09-01

    Clostridium perfringens can produce up to three different sialidases, including NanI, its major exosialidase. The current study first showed that human intestinal strains of C. perfringens can grow by utilizing either glucose or sialic acids, such as N -acetylneuraminic acid (Neu5Ac), which are the end products of sialidase activity. For the human enteropathogenic strain F4969, it was then determined that culture supernatant sialidase activity and expression of exosialidase genes, particularly nanI , are influenced by the presence of Neu5Ac or glucose. Low Neu5Ac concentrations increased culture supernatant sialidase activity, largely by stimulating nanI transcription. In contrast, low glucose concentrations did not affect exosialidase activity or nanI transcription. However, either high Neu5Ac or high glucose concentrations repressed F4969 culture supernatant sialidase activity and nanI transcription levels. Furthermore, high glucose levels repressed F4969 culture sialidase activity and nanI expression even in the presence of low Neu5AC concentrations. To begin to evaluate the mechanistic basis for nanI expression, a nanR null mutant was used to demonstrate that NanR, a member of the RpiR family of regulatory proteins, decreases exosialidase activity and nanI transcription in the absence of sialic acid. The ability of C. perfringens to regulate its exosialidase activity, largely by controlling nanI expression, may affect intestinal pathogenesis by affecting the production of NanI, which may affect C. perfringens growth, adhesion, and toxin binding in vivo . Copyright © 2017 American Society for Microbiology.

  2. Transformative Power of Digital Citizenship: Critical Perspectives on Culture, New Media and Pedagogy

    ERIC Educational Resources Information Center

    Kurubacak, Gulsun

    2007-01-01

    This paper discusses culture, as a source of conflict than of synergy, and how it affects the use of new media to build digital citizenships. It also argues that the cultural dimensions of Geert Hofsteded, who demonstrates that there are national and regional cultural groupings that affect behavior of organizations, are very persistent across…

  3. Influence of lactose hydrolysis on galacto-oligosaccharides, lactose, volatile profile and physicochemical parameters of different yogurt varieties.

    PubMed

    Vénica, Claudia I; Wolf, Irma V; Bergamini, Carina V; Perotti, María C

    2016-12-01

    Different types of reduced-lactose yogurt, obtained by lactose hydrolysis using β-galactosidase enzyme, are commercially available. The breakdown of lactose modifies the carbohydrate profile, including the production of prebiotic galacto-oligosaccharides (GOS), which could affect the survival and activity of starter and probiotic cultures and the parameters of yogurt quality. The extension of these changes is dependent on the yogurt matrix composition. This study aimed to evaluate the influence of lactose hydrolysis on GOS, lactose, volatile profile and physicochemical parameters of different yogurt varieties during storage. The presence of β-galactosidase enzyme did not affect either the global composition or the survival of cultures. Overall, the hydrolyzed products had lower acidity than traditional ones. GOS were found at similar levels in fresh hydrolyzed yogurts, whereas in traditional yogurts they were not detected. The proportion of ketones, acids and aldehydes seems to be more dependent on yogurt variety than on addition of the enzyme. Likewise, the storage period affected the volatile fraction to different degree; the increase in acid compounds was more pronounced in hydrolyzed than in traditional yogurts. This work shows that it is possible to obtain different varieties of reduced-lactose yogurt, some of them with additional benefits to health such as reduced fat, reduced calories, added with probiotic/inulin and enriched in GOS, with similar characteristics to traditional products. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  4. The effect of cultural interaction on cumulative cultural evolution.

    PubMed

    Nakahashi, Wataru

    2014-07-07

    Cultural transmission and cultural evolution are important for animals, especially for humans. I developed a new analytical model of cultural evolution, in which each newborn learns cultural traits from multiple individuals (exemplars) in parental generation, individually explores around learned cultural traits, judges the utility of known cultural traits, and adopts a mature cultural trait. Cultural evolutionary speed increases when individuals explore a wider range of cultural traits, accurately judge the skill level of cultural traits (strong direct bias), do not strongly conform to the population mean, increase the exploration range according to the variety of socially learned cultural traits (condition dependent exploration), and make smaller errors in social learning. Number of exemplars, population size, similarity of cultural traits between exemplars, and one-to-many transmission have little effect on cultural evolutionary speed. I also investigated how cultural interaction between two populations with different mean skill levels affects their cultural evolution. A population sometimes increases in skill level more if it encounters a less skilled population than if it does not encounter anyone. A less skilled population sometimes exceeds a more skilled population in skill level by cultural interaction between both populations. The appropriateness of this analytical method is confirmed by individual-based simulations. Copyright © 2014 Elsevier Ltd. All rights reserved.

  5. Teaching Writing

    ERIC Educational Resources Information Center

    Tomas, Z.; Kostka, I.; Mott-Smith, J. A.

    2013-01-01

    The authors of "Teaching Writing" draw on their years of teaching and their knowledge of theory and research to present major concepts in teaching L2 writing. These concepts encompass how cultural differences affect the writing class, planning instruction, text-based writing, writing strategies, modeling, and responding to student…

  6. Childbirth customs in Vietnamese traditions.

    PubMed Central

    Bodo, K.; Gibson, N.

    1999-01-01

    OBJECTIVE: To examine and understand how differences in the cultural backgrounds of Canadian physicians and their Vietnamese patients can affect the quality and efficacy of prenatal and postnatal treatment. QUALITY OF EVIDENCE: The information in this paper is based on a review of the literature, supplemented by interviews with members of the Vietnamese community in Edmonton, Alta. The literature was searched with MEDLINE (1966 to present), HEALTHSTAR (1975 to present), EMBASE (1988 to present), and Social Sciences Abstracts (1984 to present). Emphasis was placed on articles and other texts that dealt with Vietnamese customs surrounding childbirth, but information on health and health care customs was also considered. Interviews focused on the accuracy of information obtained from the research and the correlation of those data with personal experiences of Vietnamese community members. MAIN MESSAGE: Information in the texts used to research this paper suggests that traditional Vietnamese beliefs and practices surrounding birth are very different from the biomedical view of the Canadian medical system. The experiences and beliefs of the members of the Vietnamese community support this finding. Such cultural differences could contribute to misunderstandings between physicians and patients and could affect the quality and efficacy of health care provided. CONCLUSIONS: A sensitive and open approach to the patient's belief system and open and frank communication are necessary to ensure effective prenatal and postnatal treatment for recent Vietnamese immigrants and refugees. Education and awareness of cultural differences are necessary for physicians to provide the best and most effective health care possible. Images p692-a PMID:10099808

  7. Rethinking the globalisation of problem-based learning: how culture challenges self-directed learning.

    PubMed

    Frambach, Janneke M; Driessen, Erik W; Chan, Li-Chong; van der Vleuten, Cees P M

    2012-08-01

    Medical schools worldwide are increasingly switching to student-centred methods such as problem-based learning (PBL) to foster lifelong self-directed learning (SDL). The cross-cultural applicability of these methods has been questioned because of their Western origins and because education contexts and learning approaches differ across cultures. This study evaluated PBL's cross-cultural applicability by investigating how it is applied in three medical schools in regions with different cultures in, respectively, East Asia, the Middle East and Western Europe. Specifically, it investigated how students' cultural backgrounds impact on SDL in PBL and how this impact affects students. A qualitative, cross-cultural, comparative case study was conducted in three medical schools. Data were collected through 88 semi-structured, in-depth interviews with Year 1 and 3 students, tutors and key persons involved in PBL, 32 observations of Year 1 and 3 PBL tutorials, document analysis, and contextual information. The data were thematically analysed using the template analysis method. Comparisons were made among the three medical schools and between Year 1 and 3 students across and within the schools. The cultural factors of uncertainty and tradition posed a challenge to Middle Eastern students' SDL. Hierarchy posed a challenge to Asian students and achievement impacted on both sets of non-Western students. These factors were less applicable to European students, although the latter did experience some challenges. Several contextual factors inhibited or enhanced SDL across the cases. As students grew used to PBL, SDL skills increased across the cases, albeit to different degrees. Although cultural factors can pose a challenge to the application of PBL in non-Western settings, it appears that PBL can be applied in different cultural contexts. However, its globalisation does not postulate uniform processes and outcomes, and culturally sensitive alternatives might be developed. © Blackwell Publishing Ltd 2012.

  8. When ancestral heritage is a source of discomfort: culture, pre-object relatedness, and self-alienation.

    PubMed

    Kradin, Richard L

    2012-04-01

    The ancestral claims on an individual can evoke mental conflict when they involve separating from an ethnic group whose beliefs and customs are devalued by the dominant culture. However, these claims are engraved on the psyche early in development by caretakers to the level of pre-object relatedness, where contents and affect tones are implicit and may be unavailable for later psychoanalytical interventions. In addition, as the anthropologist Clifford Geertz notes, one's culture of origin precedes the development of psyche and creates its own set of claims that must be renegotiated when one encounters a different domain of cultural symbols, a confrontation that can produce psychological dissonance and self-alienation. In this paper, three cases are examined in which mental conflicts were evoked by attempts at divesting ancestral claims in response to conscious efforts to assimilate into the dominant culture. These patients suffered from separation guilt and unstable self-esteem and reported dream imagery suggesting psychological imbalance. The requirement to carefully delineate the ancestral claims on psyche as well as those contents and affects that may not be accessible to therapeutic intervention is emphasized, and the importance of compromise and acceptance with respect to the psychological demands of the unconscious are considered. 2012, The Society of Analytical Psychology.

  9. Analysis and Characterization of 3-(3,4-Dichlorophenyl)-1,1-Dimethylurea (DCMU)-resistant Euglena

    PubMed Central

    Calvayrac, Régis; Bomsel, Jean-Loup; Laval-Martin, Danielle

    1979-01-01

    Cultures of Euglena gracilis Klebs strain Z Pringsheim were grown photoorganotrophically in the presence of different concentrations of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) in the range of 0.05 to 250 micromolar. Cultures were serially transferred and various metabolic parameters were followed for 10 weeks. A process of adaptation occurred which was divided operationally into three phases. A phase of ultrastructural disorganization occurred, succeeded by a recovery phase; their intensity and duration were functions of the dose of DCMU. A stable adaptation phase then ensued. This phase was observed in all cultures except that exposed to the highest DCMU concentration. Adapted cells from all of the DCMU cultures contained twice the protein and half the paramylon of the control cells and thus utilized the carbon source to accumulate cellular reserves with only half the efficiency of controls. DCMU affected cellular metabolism as well as photosynthesis. The energy charge remained at high levels throughout adaptation, although the size of the adenylate pool was half that of controls at the disorganized phase. At this stage the ultrastructure of chloroplasts and mitochondria was considerably modified. The progressive changes of the parameters studied appeared to affect all of the cells in a given culture. Images PMID:16660827

  10. Neural evidence for cultural differences in the valuation of positive facial expressions

    PubMed Central

    Park, BoKyung; Chim, Louise; Blevins, Elizabeth; Knutson, Brian

    2016-01-01

    European Americans value excitement more and calm less than Chinese. Within cultures, European Americans value excited and calm states similarly, whereas Chinese value calm more than excited states. To examine how these cultural differences influence people’s immediate responses to excited vs calm facial expressions, we combined a facial rating task with functional magnetic resonance imaging. During scanning, European American (n = 19) and Chinese (n = 19) females viewed and rated faces that varied by expression (excited, calm), ethnicity (White, Asian) and gender (male, female). As predicted, European Americans showed greater activity in circuits associated with affect and reward (bilateral ventral striatum, left caudate) while viewing excited vs calm expressions than did Chinese. Within cultures, European Americans responded to excited vs calm expressions similarly, whereas Chinese showed greater activity in these circuits in response to calm vs excited expressions regardless of targets’ ethnicity or gender. Across cultural groups, greater ventral striatal activity while viewing excited vs. calm expressions predicted greater preference for excited vs calm expressions months later. These findings provide neural evidence that people find viewing the specific positive facial expressions valued by their cultures to be rewarding and relevant. PMID:26342220

  11. Anxiety disorders through a transcultural perspective: implications for migrants.

    PubMed

    Agorastos, Agorastos; Haasen, Christian; Huber, Christian G

    2012-01-01

    This paper analyses the psychopathology and differential diagnosis of anxiety disorders and their worldwide prevalence. It focuses particularly on migrant-specific aetiopathogenetic factors and approaches prevalence and important cultural aspects of anxiety disorders in migrants as a narrative empirical review. Transcultural research demonstrates the universal existence of anxiety disorders. However, the cross-cultural comparison of the epidemiological data is complicated and often leads to systematic bias. The described psychopathology varies significantly across cultures, indicating different ways of expressing and experiencing the basic emotion of anxiety. As a result, anxiety disorders manifest themselves differently across cultures, which may affect the diagnostic and therapeutic procedure. Migration is an additional extraordinary stressor playing a substantial role in the development of anxiety disorders. However, despite the high prevalence of anxiety disorders among migrants, a high threshold due to several barriers leads to an underrepresentation of migrants in the utilization of the mental health system. In the transcultural differential diagnosis of anxiety disorders, as among migrants, understanding and openness to other cultures and their standards is essential. Thereby, a migrant-specific treatment approach with a long-term binding of patients in a multimodal and culture-sensitive and -permissive treatment option is of vital importance. Copyright © 2012 S. Karger AG, Basel.

  12. A cross-cultural analysis of posthumous reproduction: The significance of the gender and margins-of-life perspectives.

    PubMed

    Hashiloni-Dolev, Yael; Schicktanz, Silke

    2017-06-01

    The scholarly discussion of posthumous reproduction (PHR) focuses on informed consent and the welfare of the future child, for the most part overlooking cultural differences between societies. Based on a cross-cultural comparison of legal and regulatory documents, analysis of pivotal cases and study of scholarly and media discussions in Israel and Germany, this paper analyses the relevant ethical and policy issues, and questions how cultural differences shape the practice of PHR. The findings challenge the common classifications of PHR by highlighting the gender perspective and adding brain-dead pregnant women to the debate. Based on this study's findings, four neglected cultural factors affecting social attitudes towards PHR are identified: (i) the relationship between the pregnant woman and her future child; (ii) what constitutes the beginning of life; (iii) what constitutes dying; and (iv) the social agent(s) seeking to have the future child. The paper argues that PHR can be better understood by adding the gender and margins-of-life perspectives, and that future ethical and practical discussions of this issue could benefit from the criteria emerging from this cross-cultural analysis.

  13. Reflections on International Certified Nursing Assistants.

    PubMed

    Shaw, Penelope Ann

    2017-01-01

    The author, a former university faculty member who taught English to speakers of other languages and now a nursing home resident, shares her observations about how English language proficiency, culture, and religious differences affect her care. She provides examples of communication challenges that can be annoying or cause harm, her coping strategies, and reasons many certified nursing assistants might never be fully fluent in English. She explains how international certified nursing assistants can benefit residents because of skills developed by family-centered care in their countries of origin. She also discusses related issues-the importance of being culturally competent about U.S. culture. She points out how religiousness not only affects residents but is a buffer for staff against the stress of physically and emotionally demanding low-wage work. Overall, the author likes receiving care from individuals from other countries, finding reward in comparing how her personal struggle with illness and paralysis resonates with the trauma of migration and how learning firsthand about varying beliefs and attitudes clarifies her identity and place in world history.

  14. Mild hypothermic culture conditions affect residual host cell protein composition post-Protein A chromatography

    PubMed Central

    Goey, Cher Hui; Bell, David; Kontoravdi, Cleo

    2018-01-01

    ABSTRACT Host cell proteins (HCPs) are endogenous impurities, and their proteolytic and binding properties can compromise the integrity, and, hence, the stability and efficacy of recombinant therapeutic proteins such as monoclonal antibodies (mAbs). Nonetheless, purification of mAbs currently presents a challenge because they often co-elute with certain HCP species during the capture step of protein A affinity chromatography. A Quality-by-Design (QbD) strategy to overcome this challenge involves identifying residual HCPs and tracing their source to the harvested cell culture fluid (HCCF) and the corresponding cell culture operating parameters. Then, problematic HCPs in HCCF may be reduced by cell engineering or culture process optimization. Here, we present experimental results linking cell culture temperature and post-protein A residual HCP profile. We had previously reported that Chinese hamster ovary cell cultures conducted at standard physiological temperature and with a shift to mild hypothermia on day 5 produced HCCF of comparable product titer and HCP concentration, but with considerably different HCP composition. In this study, we show that differences in HCP variety at harvest cascaded to downstream purification where different residual HCPs were present in the two sets of samples post-protein A purification. To detect low-abundant residual HCPs, we designed a looping liquid chromatography-mass spectrometry method with continuous expansion of a preferred, exclude, and targeted peptide list. Mild hypothermic cultures produced 20% more residual HCP species, especially cell membrane proteins, distinct from the control. Critically, we identified that half of the potentially immunogenic residual HCP species were different between the two sets of samples. PMID:29381421

  15. Cell culture density affects the stemness gene expression of adipose tissue-derived mesenchymal stem cells.

    PubMed

    Kim, Dae Seong; Lee, Myoung Woo; Lee, Tae-Hee; Sung, Ki Woong; Koo, Hong Hoe; Yoo, Keon Hee

    2017-03-01

    The results of clinical trials using mesenchymal stem cells (MSCs) are controversial due to the heterogeneity of human MSCs and differences in culture conditions. In this regard, it is important to identify gene expression patterns according to culture conditions, and to determine how the cells are expanded and when they should be clinically used. In the current study, stemness gene expression was investigated in adipose tissue-derived MSCs (AT-MSCs) harvested following culture at different densities. AT-MSCs were plated at a density of 200 or 5,000 cells/cm 2 . After 7 days of culture, stemness gene expression was examined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis. The proliferation rate of AT-MSCs harvested at a low density (~50% confluent) was higher than that of AT-MSCs harvested at a high density (~90% confluent). Although there were differences in the expression levels of stemness gene, such as octamer-binding transcription factor 4, nanog homeobox ( Nanog ), SRY-box 2, Kruppel like factor 4, v-myc avian myelocytomatosis viral oncogene homolog ( c-Myc ), and lin-28 homolog A, in the AT-MSCs obtained from different donors, RT-qPCR analysis demonstrated differential gene expression patterns according to the cell culture density. Expression levels of stemness genes, particularly Nanog and c-Myc , were upregulated in AT-MSCs harvested at a low density (~50% confluent) in comparison to AT-MSCs from the same donor harvested at a high density (~90% confluent). These results imply that culture conditions, such as the cell density at harvesting, modulate the stemness gene expression and proliferation of MSCs.

  16. Mild hypothermic culture conditions affect residual host cell protein composition post-Protein A chromatography.

    PubMed

    Goey, Cher Hui; Bell, David; Kontoravdi, Cleo

    2018-04-01

    Host cell proteins (HCPs) are endogenous impurities, and their proteolytic and binding properties can compromise the integrity, and, hence, the stability and efficacy of recombinant therapeutic proteins such as monoclonal antibodies (mAbs). Nonetheless, purification of mAbs currently presents a challenge because they often co-elute with certain HCP species during the capture step of protein A affinity chromatography. A Quality-by-Design (QbD) strategy to overcome this challenge involves identifying residual HCPs and tracing their source to the harvested cell culture fluid (HCCF) and the corresponding cell culture operating parameters. Then, problematic HCPs in HCCF may be reduced by cell engineering or culture process optimization. Here, we present experimental results linking cell culture temperature and post-protein A residual HCP profile. We had previously reported that Chinese hamster ovary cell cultures conducted at standard physiological temperature and with a shift to mild hypothermia on day 5 produced HCCF of comparable product titer and HCP concentration, but with considerably different HCP composition. In this study, we show that differences in HCP variety at harvest cascaded to downstream purification where different residual HCPs were present in the two sets of samples post-protein A purification. To detect low-abundant residual HCPs, we designed a looping liquid chromatography-mass spectrometry method with continuous expansion of a preferred, exclude, and targeted peptide list. Mild hypothermic cultures produced 20% more residual HCP species, especially cell membrane proteins, distinct from the control. Critically, we identified that half of the potentially immunogenic residual HCP species were different between the two sets of samples.

  17. Comparing the importance of different aspects of quality of life to older adults across diverse cultures.

    PubMed

    Molzahn, Anita E; Kalfoss, Mary; Schick Makaroff, Kara; Skevington, Suzanne M

    2011-03-01

    there is limited research examining the relative importance of aspects of quality of life (QOL) to older adults across cultures. to examine the relative importance of 31 internationally agreed areas of QOL to older adults in 22 countries in relation to health status, age and level of economic development. a survey quota sampling design was used to collect cross-cultural data. This study reports a secondary analysis of WHOQOL-OLD pilot study, which was collected simultaneously in 22 centres. a variety of community, primary, secondary and tertiary health care settings located in Australia, France, Switzerland, England, Scotland, USA, Israel, Spain, Japan, China (mainland and Hong Kong), Turkey, Lithuania, Czech Republic, Hungary, Canada, Norway, Sweden, Denmark, Germany, Brazil and Uruguay. the total sample contained 7,401 people over 60 years with a mean age of 73.1 years; 57.8% were women and 70.1% considered themselves 'healthy'. there were significant differences in the importance given to various aspects of QOL for people living in medium and high-development countries. Culture explained 15.9% of the variance in the importance ratings of QOL. However, the interaction showed that cultural differences were reduced once health status, gender and age were taken into account. The importance of QOL to age bands in different cultures was not significantly affected by whether or not participants perceived themselves to be healthy. understanding the self-reported importance of diverse aspects of QOL for different cultures and for healthy and less healthy people may assist national and international policy makers to decide on priorities for the development of programmes for the ageing population.

  18. Congruence and functions of personal and cultural values: do my values reflect my culture's values?

    PubMed

    Fischer, Ronald

    2006-11-01

    Two studies are described examining the correlation between self- and culture-referenced values at a culture level (Study 1) and correlation between self- and culture-referenced values and self-reported behavior at an individual level (Study 2). It is found that values related to individual-group relationships (embeddedness) and expression and experience of affective feelings and emotions (affective autonomy) are significantly correlated at a culture level. In Study 2, culture-referenced values are shown to correlate with behaviors attached to social norms, whereas self-rated values are found to correlate with behaviors that are not norm-governed. Implications for measurement of cultural values and cultural and cross-cultural research designs are discussed.

  19. The Structure of Musical Preferences: A Five-Factor Model

    PubMed Central

    Rentfrow, Peter J.; Goldberg, Lewis R.; Levitin, Daniel J.

    2011-01-01

    Music is a cross-cultural universal, a ubiquitous activity found in every known human culture. Individuals demonstrate manifestly different preferences in music, and yet relatively little is known about the underlying structure of those preferences. Here, we introduce a model of musical preferences based on listeners’ affective reactions to excerpts of music from a wide variety of musical genres. The findings from three independent studies converged to suggest that there exists a latent five-factor structure underlying music preferences that is genre-free, and reflects primarily emotional/affective responses to music. We have interpreted and labeled these factors as: 1) a Mellow factor comprising smooth and relaxing styles; 2) an Urban factor defined largely by rhythmic and percussive music, such as is found in rap, funk, and acid jazz; 3) a Sophisticated factor that includes classical, operatic, world, and jazz; 4) an Intense factor defined by loud, forceful, and energetic music; and 5) a Campestral factor comprising a variety of different styles of direct, and rootsy music such as is often found in country and singer-songwriter genres. The findings from a fourth study suggest that preferences for the MUSIC factors are affected by both the social and auditory characteristics of the music. PMID:21299309

  20. An evaluation of factors which can affect the implementation of a health promotion programme under the Schools for Health in Europe framework.

    PubMed

    Bennett, Annemarie E; Cunningham, Cara; Johnston Molloy, Charlotte

    2016-08-01

    The Health Promoting Schools concept helps schools to promote health in a sustainable and long-term fashion. However, developing the capacity to promote health in this way can be challenging when a busy teaching curriculum must be fulfilled. This study aimed to identify factors which affect the acceptability of health promotion programmes to the everyday school environment. Semi-structured qualitative interviews were audio-taped with primary school teachers in one Irish county and transcribed verbatim. The resulting transcripts were analysed using content analysis. Thirty-one teachers were interviewed. The factors which may adversely affect the acceptability of health promotion programmes include the: attitude of teachers towards an additional extra-curricular workload; lack of confidence amongst teachers to lead health promotion; and different organisational cultures between schools. When health promotion programmes under the Health Promoting Schools concept are being implemented, it's important to consider: the readiness for change amongst teachers; the resources available to increase staff capacity to promote health; and the ability of a programme to adapt to the different organisational cultures between schools. Copyright © 2016 Elsevier Ltd. All rights reserved.

  1. Comparative Metabolomic Analysis of the Green Microalga Chlorella sorokiniana Cultivated in the Single Culture and a Consortium with Bacteria for Wastewater Remediation.

    PubMed

    Chen, Taojing; Zhao, Quanyu; Wang, Liang; Xu, Yunfeng; Wei, Wei

    2017-11-01

    Co-culture of microalgae with many types of bacteria usually comes out with significant different treatment efficiencies for COD, nitrogen, and phosphorus in wastewater remediation, compared with the single culture. In order to understand the mechanism behind, a comparative experiment was designed in this study, using the green microalgae species Chlorella sorokiniana in the single culture and a consortium with a bacterium, Pseudomonas H4, for nutrient removal. Comparative metabolome profile analysis was conducted to reveal the Chlorella cell responses to the synergistic growth with the bacteria, and possible relations between the metabolic regulation of microalgae and the nutrient degradation were discussed. The detectable differential metabolites of Chlorella belonged to several classes, including carbohydrates, fatty acids, amino acids, phosphates, polyols, etc. The orthogonal partial least squares discriminant analysis (OPLS-DA) model of the identified metabolites suggests the metabolism in this alga was significantly affected by the bacteria, corresponding to different treatment behaviors.

  2. Nerve growth factor regulates galanin and neuropeptide Y expression in primary cultured superior cervical ganglion neurons.

    PubMed

    Liu, Huaxiang; Liu, Zhen; Xu, Xiaobo; Yang, Xiangdong; Wang, Huaijing; Li, Zhengzhong

    2010-03-01

    Both galanin and neuropeptide Y (NPY) are expressed in superior cervical ganglion (SCG) neurons. Following nerve transection or axotomy galanin is strongly upregulated and NPY is downregulated in SCG neurons because target-derived nerve growth factor (NGF) content decreased. It is not known whether or to what extent NGF affects both galanin and NPY expression in primary cultured SCG neurons. In the present study we examine whether exogenous NGF affects expression of neuropeptides for galanin and NPY in primary cultured SCG neurons. In addition, we explore whether mRNAs for galanin and NPY are affected by administration of exogenous NGF in SCG cultures. The significance of expression of galanin and NPY and their mRNAs was revealed by performing experiments without and with administration of exogenous NGF. Galanin and its mRNA expression was attenuated by administration of exogenous NGF in SCG cultures. The enhancement of NPY and its mRNA expression by administration of exogenous NGF in SCG cultures was dose-dependent. The physiological or pathophysiological mechanisms of the alterations of galanin and NPY expression affected by NGF in primary cultured SCG neurons are still unknown. The present data provide basic knowledge about the expression of galanin and NPY in primary cultured SCG neurons of rats, which may further improve our understanding of the functional significance of galanin and NPY expression affected by NGF.

  3. A cross-cultural comparison study of depression assessments conducted in Japan

    PubMed Central

    2013-01-01

    Background The advent of global clinical trials has necessitated the use of English-based rating instruments in diverse cultures where English is clearly not the primary language. The cross-cultural applicability of rating instruments developed in one language with only one cultural group is an important issue in both research and clinical settings where these instruments might be used. We examined the cross-cultural applicability of the Montgomery-Asberg Depression Rating Scale (MADRS) in Japan. Methods As part of a rater-training program for a clinical trial in Japan, we assessed inter-rater agreement using two videotaped MADRS interviews administered in Japanese and produced with English subtitles. We looked for possible interpretational variance that might have been generated by cultural differences between Japanese raters in Japan and English-speaking raters in the USA scoring the same interviews. Results The US and Japanese raters demonstrated high inter-rater agreement and no significant scoring difference on the total MADRS score. The subtitles in English did not adversely affect the overall scoring. We separately analyzed the 10 individual items from each of the two MADRS interviews used for rater training. Of the 20 items, 18 were concordant between the US and Japanese raters. In one interview, the US raters scored lassitude significantly higher (p = 0.013) and the inability to feel significantly lower (p = 0.037) than the Japanese raters, reflecting a possible interpretational difference on these items. Conclusion Although developed in Europe, these findings support the general applicability of the MADRS to assess the severity of depressive symptoms in Japan. We did note significant scoring differences on 2 of the 20 individual items, suggesting a possible cultural difference. It is possible that more interviews might have revealed more interpretational differences. These findings highlight the need for cultural familiarity when assessing psychiatric patients. PMID:23551964

  4. South East Asia, Differing Socioeconomic Factors, Differing Access to Radiotherapy: The Philippines, a Microcosm.

    PubMed

    Calaguas, Miriam Joy C; Gubat, Johannes A

    2017-04-01

    The accessibility of radiotherapy in Southeast Asia, a region characterized by diversity in both economy and culture, differ widely among its member countries. In most countries, access is not universal and is generally limited by radiotherapy resources and the health-seeking behavior of patients. This in turn relates to the current health policy in place, the practice of radiation oncology, and cultural factors such as religion and family dynamics. Although it is clear that radiotherapy access parallel economic development, it is also affected by different social factors that work to facilitate or impede its improvement. These factors are herein described in relation to the Philippine situation, where there is wide economic disparity, multiple subcultures, and logistical impediments to improving radiotherapy access, reminiscent of the Southeast Asian region. In the face of increasing cancer burden, it becomes increasingly important to improve services and develop new and efficient approaches taking different cultural factors into consideration. Through regional and international cooperation, solutions to the common and complex problem of inequity in radiotherapy access may be developed. Copyright © 2017. Published by Elsevier Inc.

  5. ALK5 inhibition maintains islet endothelial cell survival but does not enhance islet graft revascularisation or function.

    PubMed

    King, A J F; Clarkin, C E; Austin, A L F; Ajram, L; Dhunna, J K; Jamil, M O; Ditta, S I; Ibrahim, S; Raza, Z; Jones, P M

    2015-01-01

    Islet transplantation is a potential treatment for Type 1 diabetes but long term graft function is suboptimal. The rich supply of intraislet endothelial cells diminishes rapidly after islet isolation and culture, which affects the revascularisation rate of islets after transplantation. The ALK5 pathway inhibits endothelial cell proliferation and thus inhibiting ALK5 is a potential target for improving endothelial cell survival. The aim of the study was to establish whether ALK5 inhibition prevents the loss of intraislet endothelial cells during islet culture and thus improves the functional survival of transplanted islets by enhancing their subsequent revascularisation after implantation. Islets were cultured for 48 h in the absence or presence of 2 different ALK inhibitors: SB-431542 or A-83-01. Their vascular density after culture was analysed using immunohistochemistry. Islets pre-cultured with the ALK5 inhibitors were implanted into streptozotocin-diabetic mice for either 3 or 7 days and blood glucose concentrations were monitored and vascular densities of the grafts were analysed. Islets cultured with ALK5 inhibitors had higher vascular densities than control-cultured islets. Three days after implantation, endothelial cell numbers in islet grafts were minimal, irrespective of treatment during culture. Seven days after implantation, endothelial cells were evident within the islet grafts but there was no difference between control-cultured islets and islets pre-treated with an ALK5 inhibitor. Blood glucose concentrations were no different between the treatment groups. In conclusion, inhibition of ALK5 improved intraislet endothelial cell numbers after islet culture, but this effect was lost in the early post-transplantation period. © Georg Thieme Verlag KG Stuttgart · New York.

  6. On the Intentionality of Cultural Products: Representations of Black History As Psychological Affordances

    PubMed Central

    Salter, Phia S.; Adams, Glenn

    2016-01-01

    A cultural-psychological analysis emphasizes the intentionality of everyday worlds: the idea that material products not only bear psychological traces of culturally constituted beliefs and desires, but also subsequently afford and promote culturally consistent understandings and actions. We applied this conceptual framework of mutual constitution in a research project using quantitative and qualitative approaches to understand the dynamic resonance between sociocultural variance in Black History Month (BHM) representations and the reproduction of racial inequality in the U.S. In studies 1 and 2, we considered whether mainstream BHM artifacts reflect the preferences and understandings of White Americans (i.e., psychological constitution of cultural worlds). Consistent with the psychological constitution hypothesis, White American participants reported more positive affect, better recognition, and greater liking for BHM representations from the schools where White Americans were the majority than BHM representations from the schools where Black students and other students of color were the majority. Moreover, as an indication of the identity relevance of BHM representations, White identification was more positively associated with judgments of positive affect and preference in response to BHM representations from White schools than BHM representations from the schools where Black students were in the majority. In studies 3 and 4, we considered whether BHM representations from different settings differentially afford support or opposition to anti-racism policies (i.e., cultural constitution of psychological experience). In support of the cultural constitution hypothesis, BHM representations typical of schools where Black students were in the majority were more effective at promoting support for anti-racism policies compared to BHM representations typical of predominately White schools and a control condition. This effect was mediated by the effect of (different) BHM representations on perception of racism. Together, these studies suggest that representations of Black History constitute cultural affordances that, depending on their source, can promote (or impede) perception of racism and anti-racism efforts. This research contributes to an emerging body of work examining the bidirectional, psychological importance of cultural products. We discuss implications for theorizing collective manifestations of mind. PMID:27621712

  7. Cultural modulation of self-referential brain activity for personality traits and social identities.

    PubMed

    Sul, Sunhae; Choi, Incheol; Kang, Pyungwon

    2012-01-01

    Cross-cultural studies have shown that personality traits are less central and social identities are more important to the selfhood of collectivistic people. However, most cultural neuroscience studies using the self-reference effect (SRE) paradigm have only used personality traits to explore cultural differences in the neural circuits of self-referential processes. In the present study, we used both personality traits and social identities as stimuli in the SRE paradigm and investigated whether and how one's cultural orientation (i.e., individualism vs. collectivism) affects the SRE in the brain. The results showed that the medial prefrontal cortex, anterior cingulate, bilateral temporoparietal regions, and precuneus were involved in self-representation for both personality traits and social identities. Importantly, cultural orientation predicted differential activation patterns in these regions. Collectivists showed stronger activation in the left temporoparietal regions than individualists, who mainly recruited the medial prefrontal regions. Our findings suggest that the personal and social self share common neural substrates, the activation of which can be modulated by one's cultural orientation.

  8. Transformative Power of Digital Citizenship: Critical Perspectives on Culture, New Media and Pedagogy

    ERIC Educational Resources Information Center

    Kurubacak, Gulsun

    2007-01-01

    This paper discusses culture, as a source of conflict than of synergy, how affects the use of new media to build digital citizenships. It also argues that the cultural dimensions of Geert Hofstede, who demonstrates that there are national and regional cultural groupings that affect the behavior of organizations, are very persistent across time.…

  9. The effects of organizational commitment and structural empowerment on patient safety culture.

    PubMed

    Horwitz, Sujin K; Horwitz, Irwin B

    2017-03-20

    Purpose The purpose of this paper is to investigate the relationship between patient safety culture and two attitudinal constructs: affective organizational commitment and structural empowerment. In doing so, the main and interaction effects of the two constructs on the perception of patient safety culture were assessed using a cohort of physicians. Design/methodology/approach Affective commitment was measured with the Organizational Commitment Questionnaire, whereas structural empowerment was assessed with the Conditions of Work Effectiveness Questionnaire-II. The abbreviated versions of these surveys were administered to a cohort of 71 post-doctoral medical residents. For the data analysis, hierarchical regression analyses were performed for the main and interaction effects of affective commitment and structural empowerment on the perception of patient safety culture. Findings A total of 63 surveys were analyzed. The results revealed that both affective commitment and structural empowerment were positively related to patient safety culture. A potential interaction effect of the two attitudinal constructs on patient safety culture was tested but no such effect was detected. Research limitations/implications This study suggests that there are potential benefits of promoting affective commitment and structural empowerment for patient safety culture in health care organizations. By identifying the positive associations between the two constructs and patient safety culture, this study provides additional empirical support for Kanter's theoretical tenet that structural and organizational support together helps to shape the perceptions of patient safety culture. Originality/value Despite the wide recognition of employee empowerment and commitment in organizational research, there has still been a paucity of empirical studies specifically assessing their effects on patient safety culture in health care organizations. To the authors' knowledge, this study is the first empirical study to examine the relationship between structural empowerment as proposed by Kanter and the culture of patient safety using physicians.

  10. Female bovine blastocysts are more prone to apoptosis than male ones.

    PubMed

    Ghys, Emmanuelle; Dallemagne, Matthew; De Troy, Delphine; Sauvegarde, Caroline; Errachid, Abdelmounaim; Donnay, Isabelle

    2016-03-01

    Female and male embryos show differences in gene expression and metabolism from the onset of their genome. Those differences are affected by environmental factors. The objective of the study was to compare the apoptotic rates of in vitro-produced female and male bovine blastocysts cultured in different conditions. Day 7 blastocysts obtained after IVF with sex-sorted semen and culture in two synthetic oviductal fluid-based media (containing fetal calf serum [FCS] or BSA, insulin, transferrin, and selenium) were simultaneously evaluated for two markers of apoptosis after 3D reconstruction from confocal images: active caspase 3 by immunofluorescence and DNA fragmentation by terminal deoxynucleotidyl transferase dUTP nick end labeling. Higher levels of apoptotic cells were observed in female embryos whatever the culture condition but with a more pronounced difference in FCS medium. This result was confirmed using the unsexed semen of two bulls. The sex effect on apoptosis was detected in both the inner cell mass and the trophectoderm but was dependent on the embryonic size. In conclusion, this study reported that female bovine blastocysts are more prone to apoptosis than male ones but that culture in FCS exacerbates the differences in apoptosis between sexes, especially in small blastocysts. Copyright © 2016 Elsevier Inc. All rights reserved.

  11. The Cultural Turn in Sociology: Can It Help Us Resolve an Age-Old Problem in Understanding Decision Making for Health Care?

    PubMed

    Pescosolido, Bernice A; Olafsdottir, Sigrun

    2010-12-01

    Culture has long affected individuals' response to problems. A classic puzzle in the sociology of health and illness is discrepancy between theory and research regarding cultural beliefs and knowledge of medical care service use. "Utilization research," examining individuals' responses to the onset of health problems, has not consistently affected culture on the uptake of formal treatment. First, while ethnographic research often describes how culture shapes illness behaviors, survey-based studies rarely find significant beliefs or predispositions once "need" is controlled. Second, in quantitative studies, individuals report supportive treatment beliefs or predispositions to use services but low utilization levels, reinforcing claims about lack of utility of cultural ideologies in health-care decision making. We ask whether innovations in the sociology of culture and cognition provide theoretical scaffolding to conceptualize and measure culture in health service utilization. Rather than estimating effect of cultural beliefs on health-care decisionmaking, we question the measurement of cultural beliefs in understanding service use. Examining data from the General Social Survey, we focus on how approaches to culture might explain the paradox of high cultural predispositions and low actual use. Children with mental health problems provide a comparison between suggestions and endorsements. Suggestions, sources of care offered by individuals in response to a case description without any other social cues, align with new cultural approaches, and are measured by responses to open-ended questions about what should be done for the child described (with clinical criteria for ADHD, major depression, asthma, or "daily troubles"). Endorsements, requiring less cognitive work and cultural resistance, align with traditional conceptualizations of culture, and are measured by closed-ended questions that ask respondents to agree or disagree with seeking help from different treatment options placed later in the survey. Suggestions reveal cultural predispositions to use services corresponding closely to reported utilization levels; endorsements reveal high, unrealistic cultural predispositions to use services. Further, suggestions are associated with sociodemographics that proxy culture (e.g., race), while endorsements are associated only with perceived need.

  12. Rape and Child Sexual Abuse: What Beliefs Persist about Motives, Perpetrators, and Survivors?

    ERIC Educational Resources Information Center

    McGee, Hannah; O'Higgins, Madeleine; Garavan, Rebecca; Conroy, Ronan

    2011-01-01

    Rape myths are prejudicial and stereotyped beliefs about rape which persist in society. They may have a significant impact on those affected by rape as well as the performance of legal and public participants in the justice system. Rape myths may differ over time and within different societies and cultural settings. Awareness of contemporary and…

  13. Culturally Relevant Beliefs of Teachers and Their Affect on the School Experience of African American Male Students

    ERIC Educational Resources Information Center

    Davenport, Reginald O.

    2009-01-01

    The study was conducted in two suburban middle schools. These schools are located in a very diverse public school district in Middlesex County New Jersey. Like many school districts throughout this country, many African American male students are experiencing school and schooling differently based on the differences still seen in the number of…

  14. Gendered Habitus and Gender Differences in Academic Achievement

    ERIC Educational Resources Information Center

    Edgerton, Jason; Peter, Tracey; Roberts, Lance

    2014-01-01

    Bourdieu's theory of cultural and social reproduction posits that students' habitus--learned behavioural and perceptual dispositions rooted in family upbringing--is a formative influence on how they react to their educational environments, affecting academic practices and academic achievement. Although originally conceived as a "class"…

  15. APA Educational Psychology Handbook

    ERIC Educational Resources Information Center

    Harris, Karen R., Ed.; Graham, Steve, Ed.; Urdan, Tim, Ed.

    2011-01-01

    The "APA Educational Psychology Handbook" reflects the broad nature of the field today, with state-of-the-science reviews of the diverse critical theories driving research and practice; in-depth investigation of the range of individual differences and cultural/contextual factors that affect student achievement, motivation, and beliefs;…

  16. School Desegregation and Community Education: Effects and Opportunities.

    ERIC Educational Resources Information Center

    Nance, Everette E.; Dixon, James D., II

    1991-01-01

    Community education must address issues that affect a variety of cultures at different levels. Public school desegregation processes offer opportunities for restructuring schools to be more responsive to community needs, for enhancing race relations, and for improving the economic viability of a community. (JOW)

  17. The Significance of Leadership Style.

    ERIC Educational Resources Information Center

    Goldman, Elise

    1998-01-01

    In a learning environment, leadership style reflects a leader's deeply held educational beliefs, which are mirrored in the school's culture. Case studies illustrate how the leadership styles of three principals affect school ambience. Good leadership practice means acknowledging each person's differing gifts, strengths, and concerns, and utilizing…

  18. Exploring posttraumatic growth in Tamil children affected by the Indian Ocean Tsunami in 2004.

    PubMed

    Exenberger, Silvia; Ramalingam, Panch; Höfer, Stefan

    2016-10-13

    Few studies explore posttraumatic growth (PTG) in children from Eastern cultures. To help address this gap, the present study examined PTG among 177 South Indian children aged 8-17 years who were affected by the 2004 Tsunami. The study identifies the underlying factor structure of the Tamil version of the Revised Posttraumatic Growth Inventory for Children (PTGI-C-R), and aims to explore the prevalence of PTG, the relationship between distress and growth, and gender and age differences in PTG. The results of the principal component analysis indicated a two-factor structure with an interpersonal and a person-centred dimension of growth. The total scores of the Tamil PTGI-C-R were positively associated with posttraumatic stress symptoms (PTSS) and age. Moreover, there was a significant relationship between age and the person-centred growth subscale. Non-parametric tests found no gender differences in perceived growth. The role of socio-cultural factors on the nature of PTG is discussed. © 2016 International Union of Psychological Science.

  19. Active music classes in infancy enhance musical, communicative and social development.

    PubMed

    Gerry, David; Unrau, Andrea; Trainor, Laurel J

    2012-05-01

    Previous studies suggest that musical training in children can positively affect various aspects of development. However, it remains unknown as to how early in development musical experience can have an effect, the nature of any such effects, and whether different types of music experience affect development differently. We found that random assignment to 6 months of active participatory musical experience beginning at 6 months of age accelerates acquisition of culture-specific knowledge of Western tonality in comparison to a similar amount of passive exposure to music. Furthermore, infants assigned to the active musical experience showed superior development of prelinguistic communicative gestures and social behaviour compared to infants assigned to the passive musical experience. These results indicate that (1) infants can engage in meaningful musical training when appropriate pedagogical approaches are used, (2) active musical participation in infancy enhances culture-specific musical acquisition, and (3) active musical participation in infancy impacts social and communication development. © 2012 Blackwell Publishing Ltd.

  20. Colonic involvement in celiac disease and possible implications of the sigmoid mucosa organ culture in its diagnosis.

    PubMed

    Picarelli, Antonio; Di Tola, Marco; Borghini, Raffaele; Isonne, Claudia; Saponara, Annarita; Marino, Mariacatia; Casale, Rossella; Tiberti, Antonio; Pica, Roberta; Donato, Giuseppe; Frieri, Giuseppe; Corazziari, Enrico

    2013-10-01

    Celiac disease (CD), a systemic autoimmune disorder that typically involves duodenal mucosa, can also affect other intestinal areas. Duodenal and oral mucosa organ culture has already been demonstrated as a reliable procedure to identify CD. The present study investigated gluten-dependent immunological activation of colonic mucosa in CD patients. We took advantage of the numerous colonoscopies performed for various clinical conditions or only for defensive medicine. Forty-four patients with gastrointestinal symptoms or in need of colorectal cancer screening were divided into patients with serum anti-endomysium (EMA) and anti-tissue transglutaminase (anti-tTG) antibody positive results (Group A), patients with serum antibody negative results (Group B), and patients with inflammatory bowel disease (IBD) (Group C). The autoantibodies EMA and anti-tTG were evaluated in supernatants of cultured sigmoid and duodenal biopsies from patients on a gluten-containing diet. In Group A, EMA and anti-tTG resulted positive in all duodenal culture supernatants. In sigmoid culture supernatants, EMA and anti-tTG were detected in 12/16 (75 %) and 13/16 (81.3 %) patients, respectively. In Group B, none of the 17 patients showed EMA and anti-tTG positive results in both duodenal and sigmoid cultures. In Group C, all 11 patients presented EMA negative results in sigmoid cultures. Only in one patient, anti-tTG were detectable in the sigmoid culture supernatant, as expected in cases of IBD. Data confirm that the gluten-dependent immunological activation affects more intestinal tracts with different degrees of involvement, suggesting that the organ culture of colonic biopsies could represent a new tool to opportunistically detect CD.

  1. The relevance of using 3D cell cultures, in addition to 2D monolayer cultures, when evaluating breast cancer drug sensitivity and resistance

    PubMed Central

    Breslin, Susan; O'Driscoll, Lorraine

    2016-01-01

    Solid tumours naturally grow in 3D wherein the spatial arrangement of cells affects how they interact with each other. This suggests that 3D cell culture may mimic the natural in vivo setting better than traditional monolayer (2D) cell culture, where cells are grown attached to plastic. Here, using HER2-positive breast cancer cell lines as models (BT474, HCC1954, EFM192A), the effects of culturing cells in 3D using the poly-HEMA method compared to 2D cultures were assessed in terms of cellular viability, response/resistance to anti-cancer drugs, protein expression and enzyme activity. Scanning electron microscopy showed the morphology of cells in 3D to be substantially different to those cultured in 2D. Cell viability in 3D cells was substantially lower than that of cells in 2D cultures, while 3D cultures were more resistant to the effects of HER-targeted (neratinib) and classical chemotherapy (docetaxel) drugs. Expression of proteins involved in cell survival, transporters associated with drug resistance and drug targets were increased in 3D cultures. Finally, activity of drug metabolising enzyme CYP3A4 was substantially increased in 3D compared to 2D cultures. Together this data indicates that the biological information represented by 3D and 2D cell cultures is substantially different i.e. 3D cell cultures demonstrate higher innate resistance to anti-cancer drugs compared to 2D cultures, which may be facilitated by the altered receptor proteins, drug transporters and metabolising enzyme activity. This highlights the importance of considering 3D in addition to 2D culture methods in pre-clinical studies of both newer targeted and more traditional anti-cancer drugs. PMID:27304190

  2. Oxygen and tissue culture affect placental gene expression.

    PubMed

    Brew, O; Sullivan, M H F

    2017-07-01

    Placental explant culture is an important model for studying placental development and functions. We investigated the differences in placental gene expression in response to tissue culture, atmospheric and physiologic oxygen concentrations. Placental explants were collected from normal term (38-39 weeks of gestation) placentae with no previous uterine contractile activity. Placental transcriptomic expressions were evaluated with GeneChip ® Human Genome U133 Plus 2.0 arrays (Affymetrix). We uncovered sub-sets of genes that regulate response to stress, induction of apoptosis programmed cell death, mis-regulation of cell growth, proliferation, cell morphogenesis, tissue viability, and protection from apoptosis in cultured placental explants. We also identified a sub-set of genes with highly unstable pattern of expression after exposure to tissue culture. Tissue culture irrespective of oxygen concentration induced dichotomous increase in significant gene expression and increased enrichment of significant pathways and transcription factor targets (TFTs) including HIF1A. The effect was exacerbated by culture at atmospheric oxygen concentration, where further up-regulation of TFTs including PPARA, CEBPD, HOXA9 and down-regulated TFTs such as JUND/FOS suggest intrinsic heightened key biological and metabolic mechanisms such as glucose use, lipid biosynthesis, protein metabolism; apoptosis, inflammatory responses; and diminished trophoblast proliferation, differentiation, invasion, regeneration, and viability. These findings demonstrate that gene expression patterns differ between pre-culture and cultured explants, and the gene expression of explants cultured at atmospheric oxygen concentration favours stressed, pro-inflammatory and increased apoptotic transcriptomic response. Copyright © 2017 Elsevier Ltd. All rights reserved.

  3. A systematic review of measures of HIV/AIDS stigma in paediatric HIV-infected and HIV-affected populations.

    PubMed

    McAteer, Carole Ian; Truong, Nhan-Ai Thi; Aluoch, Josephine; Deathe, Andrew Roland; Nyandiko, Winstone M; Marete, Irene; Vreeman, Rachel Christine

    2016-01-01

    HIV-related stigma impacts the quality of life and care management of HIV-infected and HIV-affected individuals, but how we measure stigma and its impact on children and adolescents has less often been described. We conducted a systematic review of studies that measured HIV-related stigma with a quantitative tool in paediatric HIV-infected and HIV-affected populations. Varying measures have been used to assess stigma in paediatric populations, with most studies utilizing the full or variant form of the HIV Stigma Scale that has been validated in adult populations and utilized with paediatric populations in Africa, Asia and the United States. Other common measures included the Perceived Public Stigma Against Children Affected by HIV, primarily utilized and validated in China. Few studies implored item validation techniques with the population of interest, although scales were used in a different cultural context from the origin of the scale. Many stigma measures have been used to assess HIV stigma in paediatric populations, globally, but few have implored methods for cultural adaptation and content validity.

  4. A systematic review of measures of HIV/AIDS stigma in paediatric HIV-infected and HIV-affected populations

    PubMed Central

    McAteer, Carole Ian; Truong, Nhan-Ai Thi; Aluoch, Josephine; Deathe, Andrew Roland; Nyandiko, Winstone M; Marete, Irene; Vreeman, Rachel Christine

    2016-01-01

    Introduction HIV-related stigma impacts the quality of life and care management of HIV-infected and HIV-affected individuals, but how we measure stigma and its impact on children and adolescents has less often been described. Methods We conducted a systematic review of studies that measured HIV-related stigma with a quantitative tool in paediatric HIV-infected and HIV-affected populations. Results and discussion Varying measures have been used to assess stigma in paediatric populations, with most studies utilizing the full or variant form of the HIV Stigma Scale that has been validated in adult populations and utilized with paediatric populations in Africa, Asia and the United States. Other common measures included the Perceived Public Stigma Against Children Affected by HIV, primarily utilized and validated in China. Few studies implored item validation techniques with the population of interest, although scales were used in a different cultural context from the origin of the scale. Conclusions Many stigma measures have been used to assess HIV stigma in paediatric populations, globally, but few have implored methods for cultural adaptation and content validity. PMID:27717409

  5. Condition and biochemical profile of blue mussels (Mytilus edulis L.) cultured at different depths in a cold water coastal environment

    NASA Astrophysics Data System (ADS)

    Gallardi, Daria; Mills, Terry; Donnet, Sebastien; Parrish, Christopher C.; Murray, Harry M.

    2017-08-01

    The growth and health of cultured blue mussels (Mytilus edulis) are affected by environmental conditions. Typically, culture sites are situated in sheltered areas near shore (i.e., < 1 km distance from land, < 20 m depth); however, land runoff, user conflicts and environmental impact in coastal areas are concerns and interest in developing deep water (> 20 m depth) mussel culture has been growing. This study evaluated the effect of culture depth on blue mussels in a cold water coastal environment (Newfoundland, Canada). Culture depth was examined over two years from September 2012 to September 2014; mussels from three shallow water (5 m) and three deep water (15 m) sites were compared for growth and biochemical composition; culture depths were compared for temperature and chlorophyll a. Differences between the two years examined were noted, possibly due to harsh winter conditions in the second year of the experiment. In both years shallow and deep water mussels presented similar condition; in year 2 deep water mussels had a significantly better biochemical profile. Lipid and glycogen analyses showed seasonal variations, but no significant differences between shallow and deep water were noted. Fatty acid profiles showed a significantly higher content of omega-3 s (20:5ω3; EPA) and lower content of bacterial fatty acids in deep water sites in year 2. Everything considered, deep water appeared to provide a more favorable environment for mussel growth than shallow water under harsher weather conditions.

  6. Contacting co-culture of human retinal microvascular endothelial cells alters barrier function of human embryonic stem cell derived retinal pigment epithelial cells.

    PubMed

    Skottman, H; Muranen, J; Lähdekorpi, H; Pajula, E; Mäkelä, K; Koivusalo, L; Koistinen, A; Uusitalo, H; Kaarniranta, K; Juuti-Uusitalo, K

    2017-10-01

    Here we evaluated the effects of human retinal microvascular endothelial cells (hREC) on mature human embryonic stem cell (hESC) derived retinal pigment epithelial (RPE) cells. The hESC-RPE cells (Regea08/017, Regea08/023 or Regea11/013) and hREC (ACBRI 181) were co-cultured on opposite sides of transparent membranes for up to six weeks. Thereafter barrier function, small molecule permeability, localization of RPE and endothelial cell marker proteins, cellular fine structure, and growth factor secretion of were evaluated. After co-culture, the RPE specific CRALBP and endothelial cell specific von Willebrand factor were appropriately localized. In addition, the general morphology, pigmentation, and fine structure of hESC-RPE cells were unaffected. Co-culture increased the barrier function of hESC-RPE cells, detected both with TEER measurements and cumulative permeability of FD4 - although the differences varied among the cell lines. Co-culturing significantly altered VEGF and PEDF secretion, but again the differences were cell line specific. The results of this study showed that co-culture with hREC affects hESC-RPE functionality. In addition, co-culture revealed drastic cell line specific differences, most notably in growth factor secretion. This model has the potential to be used as an in vitro outer blood-retinal barrier model for drug permeability testing. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

  7. Exploring culture-specific differences in beliefs about causes, kinship and the heritability of major depressive disorder: the views of Anglo-Celtic and Chinese-Australians.

    PubMed

    Xu, Mimi; Zou, Lilian; Wilde, Alex; Meiser, Bettina; Barlow-Stewart, Kristine; Chan, Bibiana; Mitchell, Philip B; Sousa, Mariana S; Schofield, Peter R

    2013-10-01

    The aim of this study was to explore cultural differences in causal attributions and beliefs about heritability of major depressive disorder (MDD). Face-to-face interviews with Anglo-Celtic- and Chinese-Australians community members with a family history of MDD were conducted and subjected to a rigorous qualitative analysis, using the computer software NVivo. Sixteen Anglo-Celtic-Australians and 16 Chinese-Australians were interviewed. Both groups believed that a combination of genetic and environmental factors contributed to MDD, that stress was an important cause of MDD, and that coping factors were significant moderators of the impact of stress on MDD. Both cultural groups believed that the causes of MDD affecting multiple family members included a shared family environment and a "contagion effect", in addition to genetics. Unique to the Chinese-Australian group was the beliefs that parental pressures to exceed academically contributed to MDD; this cultural group also reported beliefs that depression was due to God's will or alternatively fate, which in turn was related to attributions to feng shui and auspicious dates. This study documented key culture-specific differences in beliefs about causes and inheritance of MDD; such differences have major implications for clinician-patient communication about genetic risk associated with having a family history of MDD.

  8. Individual differences, cultural differences, and dialectic conflict description and resolution.

    PubMed

    Kim, Kyungil; Markman, Arthur B

    2013-01-01

    Previous research suggests that members of East Asian cultures show a greater preference for dialectical thinking than do Westerners. This paper attempts to account for these differences in cognition using individual difference variables that may explain variation in performance both within and across cultures. Especially, we propose that the abovementioned cultural differences are rooted in a greater fear of isolation (FOI) in East Asians than in Westerners. To support this hypothesis, in Experiment 1, we manipulated FOI in American participants before having them resolve two conflicts: an interpersonal conflict and a conflict between an individual and an institution. We found that the Americans among whom a high level of FOI had been induced were more likely to look for a dialectical resolution than those among whom a low level had been prompted. The relationship between conflict resolution and FOI was further investigated in Experiment 2, in which FOI was not manipulated. The results indicated that Koreans had higher chronic FOI on average than did the Americans. Compared to the Americans, the Koreans were more likely to resolve the interpersonal conflict dialectically, but did not show the same bias in resolving the person-institution conflict. The differences in the preference for dialectical resolution between FOI conditions in Experiment 1 and cultural groups in Experiment 2 were mediated by FOI. These findings bolster previous research on FOI in showing that chronic levels of FOI are positively related to both preference for dialectical sentences and sensitivity to context. They provide clearer insight into how differences in FOI affect attention and thereby higher-level reasoning such as dialectic description and conflict resolution.

  9. Why attend school? Chinese immigrant and European American preschoolers' views and outcomes.

    PubMed

    Li, Jin; Yamamoto, Yoko; Luo, Lily; Batchelor, Andrea K; Bresnahan, Richard M

    2010-11-01

    The developing views of the purposes of school learning (PSLs) and related achievement among immigrant Chinese preschoolers and their European American (EA) age-mates were examined. Both culture and socioeconomic status (SES) were considered simultaneously, an often neglected research approach to studying Asian children. One hundred and fifty 4-year-olds-50 each of middle-class Chinese (CHM), low-income Chinese (CHL), and EA children-completed 2 story beginnings about school and were also tested for their language and math achievement. Results showed that 4-year-olds held sophisticated PSLs, ranging from intellectual to social and affect benefits. Large cultural and SES differences also emerged. CHM children mentioned more adult expectation and seriousness of learning than EA children who expressed more positive affect for self and compliance with adults. CHL children mentioned fewest PSLs. Achievement scores for oral expression of both immigrant groups were significantly lower than those of EA children despite similar reading and math achievement. Controlling for culture and SES, the authors found that children's articulated intellectual, but not other purposes, uniquely predicted their achievement in all tested domains. Cultural and SES influences on immigrant children are discussed.

  10. Cultural competence education for practicing physicians: lessons in cultural humility, nonjudgmental behaviors, and health beliefs elicitation.

    PubMed

    Kutob, Randa M; Bormanis, John; Crago, Marjorie; Harris, John M; Senf, Janet; Shisslak, Catherine M

    2013-01-01

    Although numerous studies have examined cultural competence training, debate still exists about efficacious approaches to this training. Furthermore, little focus has been placed on training and evaluating practicing physicians. A skills-based course on culturally competent diabetes care was developed and subsequently tested in a controlled trial of primary physicians caring for patients enrolled in one state's Medicaid program. We hypothesized that physicians completing the course would show higher levels of self-reported cultural competence as measured by a Cultural Competence Assessment Tool (CCAT) than those in the control group. Differences in CCAT subscale scores were also compared. Ninety physicians completed the study, with 41 in the control and 49 in the intervention group. Most were female (66%), with an average age of 44, and 12 years in practice. There were no significant differences on total CCAT score (212.7 ± 26.7 for control versus 217.2 ± 28.6 for intervention, p = .444) or subscales measuring cultural knowledge. There were significant positive differences on the subscales measuring physicians' nonjudgmental attitudes/behaviors (subscale score 2.38 ± 0.46 for control versus 2.69 ± 0.52 for intervention, p = .004) and future likelihood of eliciting patients' beliefs about diabetes and treatment preferences (3.11 ± 0.53 for control versus 3.37 ± 0.45 for intervention, p = .014). There was, however, a significant negative difference on the subscale measuring cultural self-awareness (3.48 ± 0.36 for control versus 3.26 ± 0.48 for intervention, p = .018). A predominantly skills-based approach to training physicians did not change aggregate measures of cultural competence, but did affect key attitudes and behaviors, which may better reflect the goals of cultural competence training. Copyright © 2013 The Alliance for Continuing Education in the Health Professions, the Society for Academic Continuing Medical Education, and the Council on CME, Association for Hospital Medical Education.

  11. Abnormal urinalysis results are common, regardless of specimen collection technique, in women without urinary tract infections.

    PubMed

    Frazee, Bradley W; Enriquez, Kayla; Ng, Valerie; Alter, Harrison

    2015-06-01

    Voided urinalysis to test for urinary tract infection (UTI) is prone to false-positive results for a number of reasons. Specimens are often collected at triage from women with any abdominal complaint, creating a low UTI prevalence population. Improper collection technique by the patient may affect the result. At least four indices, if positive, can indicate UTI. We examine the impact of voided specimen collection technique on urinalysis indicators of UTI and on urine culture contamination in disease-free women. In this crossover design, 40 menstrual-age female emergency department staff without UTI symptoms collected urine two ways: directly in a cup ("non-clean") and midstream clean catch ("ideal"). Samples underwent standard automated urinalysis and culture. Urinalysis indices and culture contamination were compared. The proportion of abnormal results from samples collected by "non-clean" vs. "ideal" technique, respectively, were: leukocyte esterase (>trace) 50%, 35% (95% confidence interval for difference -6% to 36%); nitrites (any) 2.5%, 2.5% (difference -2.5 to 2.5%); white blood cells (>5/high-powered field [HPF]) 50%, 27.5% (difference 4 to 41%); bacteria (any/HPF) 77.5%, 62.5%, (difference -7 to 37%); epithelial cells (>few) 65%, 30% (difference 13 to 56%); culture contamination (>1000 colony-forming units of commensal or >2 species) 77%, 63% (difference -5 to 35%). No urinalysis index was positively correlated with culture contamination. Contemporary automated urinalysis indices were often abnormal in a disease-free population of women, even using ideal collection technique. In clinical practice, such false-positive results could lead to false-positive UTI diagnosis. Only urine nitrite showed a high specificity. Culture contamination was common regardless of collection technique and was not predicted by urinalysis results. Copyright © 2015 Elsevier Inc. All rights reserved.

  12. Plant cell cultures: bioreactors for industrial production.

    PubMed

    Ruffoni, Barbara; Pistelli, Laura; Bertoli, Alessandra; Pistelli, Luisa

    2010-01-01

    The recent biotechnology boom has triggered increased interest in plant cell cultures, since a number of firms and academic institutions investigated intensively to rise the production of very promising bioactive compounds. In alternative to wild collection or plant cultivation, the production of useful and valuable secondary metabolites in large bioreactors is an attractive proposal; it should contribute significantly to future attempts to preserve global biodiversity and alleviate associated ecological problems. The advantages of such processes include the controlled production according to demand and a reduced man work requirement. Plant cells have been grown in different shape bioreactors, however, there are a variety of problems to be solved before this technology can be adopted on a wide scale for the production of useful plant secondary metabolites. There are different factors affecting the culture growth and secondary metabolite production in bioreactors: the gaseous atmosphere, oxygen supply and CO2 exchange, pH, minerals, carbohydrates, growth regulators, the liquid medium rheology and cell density. Moreover agitation systems and sterilization conditions may negatively influence the whole process. Many types ofbioreactors have been successfully used for cultivating transformed root cultures, depending on both different aeration system and nutrient supply. Several examples of medicinal and aromatic plant cultures were here summarized for the scale up cultivation in bioreactors.

  13. Interleukin-3 Does Not Affect the Differentiation of Mast Cells Derived from Human Bone Marrow Progenitors

    PubMed Central

    Shimizu, Yuji; Matsumoto, Kenji; Okayama, Yoshimichi; Kentaro, Sakai; Maeno, Toshitaka; Suga, Tatsuo; Miura, Toru; Takai, Shinji; Kurabayashi, Masahiko; Saito, Hirohisa

    2008-01-01

    Although IL-3 is commonly used for culture of human progenitor-derived mast cells together with Stem cell factor (SCF) and IL-6, the effect of IL-3 on human mast cell differentiation has not been well elucidated. Human bone marrow CD34+ progenitors were cultured for up to 12 weeks in the presence of rhSCF and rhIL-6 either with rhIL-3 (IL-3 (+)) or without rhIL-3 (IL-3 (−)) for the initial 1-week of culture. Total cell number increased at 2 weeks in IL-3 (+), as compared to IL-3 (−), but changes in the appearance of mast cells were delayed. When IL-3 was present for the initial 1-week culture, granules looked more mature with IL-3 than without IL-3. However, tryptase and chymase contents, and surface antigen expression (CD18, CD51, CD54, and CD117) were not altered by IL-3. Surface expression and mRNA level of FcεRIα and histamine release by crosslinking of FcεRIα did not differ from one preparation to the next. GeneChip analysis revealed that no significant differences were observed between IL-3 (+) and IL-3 (−) cells either when inactivated or activated by aggregation of FcεRIα. These findings indicate that initial incubation of human bone marrow CD34+ progenitors with IL-3 does not affect the differentiation of mast cells. PMID:18214796

  14. Collaborative Measurement Development as a Tool in CBPR: Measurement Development and Adaptation within the Cultures of Communities

    PubMed Central

    Gonzalez, John; Trickett, Edison J.

    2014-01-01

    This paper describes the processes we engaged in to develop a measurement protocol used to assess the outcomes in a community based suicide and alcohol abuse prevention project with two Alaska Native communities. While the literature on community-based participatory research (CBPR) is substantial regarding the importance of collaborations, few studies have reported on this collaboration in the process of developing measures to assess CBPR projects. We first tell a story of the processes around the standard issues of doing cross-cultural work on measurement development related to areas of equivalence. A second story is provided that highlights how community differences within the same cultural group can affect both the process and content of culturally relevant measurement selection, adaptation, and development. PMID:24748283

  15. Collaborative measurement development as a tool in CBPR: measurement development and adaptation within the cultures of communities.

    PubMed

    Gonzalez, John; Trickett, Edison J

    2014-09-01

    This paper describes the processes we engaged into develop a measurement protocol used to assess the outcomes in a community based suicide and alcohol abuse prevention project with two Alaska Native communities. While the literature on community-based participatory research (CBPR) is substantial regarding the importance of collaborations, few studies have reported on this collaboration in the process of developing measures to assess CBPR projects. We first tell a story of the processes around the standard issues of doing cross-cultural work on measurement development related to areas of equivalence. A second story is provided that highlights how community differences within the same cultural group can affect both the process and content of culturally relevant measurement selection, adaptation, and development.

  16. Heart Disease and Depression: Is Culture a Factor?

    PubMed

    Gholizadeh, Leila; Davidson, Patricia M; Heydari, Mehrdad; Salamonson, Yenna

    2014-07-01

    This article seeks to review and discuss the evidence linking depression, coronary heart disease (CHD), and culture. PsychInfo, CINAHL, PubMed, and Google were searched for pertinent evidence linking depression, culture, and CHD, and retrieved articles were analyzed using thematic content analysis. Identified themes were the followings: depression is a factor in development and prognosis of CHD and affects the capacity to self-manage and adhere to treatment recommendations; culture mediates mental health/illness representations and treatment-seeking behaviors; screening and assessment of depression can be affected by cultural factors; and there is a need for culturally appropriate screening and therapeutic strategies. As depression is a predictor and moderating variable in the genesis and progression of CHD, understanding how factors such as culture affect screening and management of the disease is important to inform the development of culturally and linguistically competent strategies that ensure accurate screening, detection, and treatment of depression in cardiac patients in clinical practice. © The Author(s) 2014.

  17. The spatial frequencies influence the aesthetic judgment of buildings transculturally.

    PubMed

    Vannucci, Manila; Gori, Simone; Kojima, Haruyuki

    2014-01-01

    Recent evidence has shown that buildings designed to be high-ranking, according to the Western architectural decorum, have more impact on the minds of their beholders than low-ranking buildings. Here we investigated whether and how the aesthetic judgment for high- and low-ranking buildings was affected by differences in cultural expertise and by power spectrum differences. A group of Italian and Japanese participants performed aesthetic judgment tasks, with line drawings of high- and low-ranking buildings and with their random-phase versions (an image with the exact power spectrum of the original one but non-recognizable anymore). Irrespective of cultural expertise, high-ranking buildings and their relative random-phase versions received higher aesthetic judgments than low-ranking buildings and their random-phase versions. These findings indicate that high- and low-ranking buildings are differentiated for their aesthetic value and they show that low-level visual processes influence the aesthetic judgment based on differences in the stimuli power spectrum, irrespective of the influence of cultural expertise.

  18. Emotion socialization practices in Italian and Hong Kong-Chinese mothers.

    PubMed

    Fiorilli, Caterina; De Stasio, Simona; Di Chicchio, Carlo; Chan, Siu Mui

    2015-01-01

    Parents' emotion socialization practices are their ways of handling their children's emotional experiences in light of cultural expectations surrounding emotions. Such practices are thought to significantly affect children's social adjustment. We compared the parenting practices of Italian and Hong Kong Chinese samples in an ethnological validation study of the maternal responses to children's emotion scale (MRCES). Participants were 71 Italian mothers (M = 39.45) and 71 Hong Kong-Chinese mothers (M = 37.75) with children aged 6-9 years. The results confirmed the two-factor structure identified by the scale's authors, namely coaching/emotion-encouraging and emotion dismissing approaches, respectively. Each of the two factors displayed satisfactory internal consistency. The Chinese mothers obtained higher scores than the Italian mothers on both subscales. Our findings suggested that parents' emotion socialization practices for coping with children's emotions received different degrees of emphasis and were underpinned by different meaning in the Hong Kong and Italian cultural groups. These cultural differences are discussed in relation to their effect on children's socio-emotional development.

  19. Give as I give: Adult influence on children's giving in two cultures.

    PubMed

    Blake, Peter R; Corbit, John; Callaghan, Tara C; Warneken, Felix

    2016-12-01

    Adult influence on children's altruistic behavior may differ between cultural communities. We used an experimental approach to assess the influence of adult models on children's altruistic giving in a city in the United States and rural villages in India. Children between 3 and 8 years of age were tested with their parents in the United States (n=163) and India (n=154). Parents modeled either a generous or stingy donation; children then performed a similar task in private. Children in both communities were influenced by the stingy model, but only children in India increased their giving after viewing a generous model. The model's influence also increased with age in India. Results of a questionnaire revealed that parents in both communities believed that children learned sharing behavior from them. We consider these results in light of differences between these societies, including different socialization goals, cultural values, and content biases that may affect altruistic giving. Copyright © 2016 Elsevier Inc. All rights reserved.

  20. Social media and interpersonal relationships: for better or worse?

    PubMed

    Quist, Norman

    2011-01-01

    Social media challenge--or have already redefined--conventional boundaries of public and private, personal and professional, friendship, and social relations generally. Here, I consider how these developments may affect professionalism, the physician-patient relationship, and our cultural experiences in a wholly different and unexpected way.

  1. Canadian and Japanese Teachers' Conceptions of Critical Thinking: A Comparative Study

    ERIC Educational Resources Information Center

    Howe, Edward R.

    2004-01-01

    Canadian and Japanese secondary teachers' conceptions of critical thinking were compared and contrasted. Significant cross-cultural differences were found. While Canadian teachers tended to relate critical thinking to the cognitive domain, Japanese teachers emphasized the affective domain. The quantitative data, effectively reduced through factor…

  2. Afro-American Patterns of Cognition.

    ERIC Educational Resources Information Center

    Shade, Barbara J.

    Reviews of literature and administration of different types of measurement tools among various student groups in Wisconsin were conducted to explore black cognitive styles on five dimensions and to investigate whether social and cultural factors affect the knowledge acquisition process. Results revealed that: (1) blacks tend to be more field…

  3. Do cultures vary in self-enhancement? ERP, behavioral, and self-report evidence.

    PubMed

    Hampton, Ryan S; Varnum, Michael E W

    2017-08-02

    Psychologists have long debated whether self-enhancement is universal or varies across cultures. Extant studies using explicit and implicit measures have provided mixed results. In this study (N = 93; 35 European American, 58 Chinese: 28 tested in English, 30 tested in Mandarin), we measured self-enhancement covertly using an ERP paradigm. Self-enhancement was also assessed via self-report and reaction-time based measures. Americans showed strong evidence of self-enhancement across all measures, whereas this effect was absent or weaker among Chinese, who instead showed an other-enhancing bias across measures. Language did not affect self-enhancement tendencies among Chinese participants, with the exception of one self-report measure. Nor did the two cultural groups differ in enhancement for a close other. This is the first study to directly compare self-enhancement across cultural groups using ERPs and provides evidence that positive self-regard does indeed vary by culture.

  4. Clean fuels from bioconversion of solar energy. Annual report, 21 January 1980-20 January 1981

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Feighner, S.D.; Sikka, H.C.

    1981-03-01

    The study seeks to enhance glycolic acid excretion by unicellular algae. The strains of algae selected to evaluate glycolic acid accumulation in culture medium were: Chlorella pyrenoidosa (UTEX 395), Chlamydomonas reinhardtii (UTEX 89), Scenedesmus obliquus (UTEX 393), and Ankistrodesmus braunii (UTEX 245). C. pyrenoidosa and C. reinhardtii, based on the amount of glycolic acid produced, were selected for further study. Initial experiments were conducted to measure the effect of different environmental growth conditions on the rate of glycolic accumulation in defined culture medium. The most pronounced effect on glycolic acid excretion was obtained by varying the concentration of carbon dioxidemore » in air. At 1% CO2 in air, C. pyrenoidosa accumulated 5.2 ppm glycolic acid in culture medium. Neither the pH of the culture medium nor the incubation temperature affected glycolic acid accumulation by growing C. pyrenoidosa cultures.« less

  5. Reactions to Receiving a Gift-Maternal Scaffolding and Cultural Learning in Berlin and Delhi.

    PubMed

    Kärtner, Joscha; Crafa, Daina; Chaudhary, Nandita; Keller, Heidi

    2016-05-01

    This study shows how Berlin (n = 35) and Delhi (n = 28) mothers scaffold a common and highly scripted social situation, namely gift giving, and enable cultural learning in 19-month-olds. Using modeling and prompting to encourage appropriate responses, mothers took culture-specific directions during scaffolding that were in line with the broader cultural model as assessed by maternal socialization goals (SGs). Whereas Berlin mothers prioritized autonomous SGs, Delhi mothers emphasized autonomous and relational SGs to similar degrees. During scaffolding, Berlin mothers focused on maximizing positive affect and acknowledging the gift, whereas Delhi mothers prompted toddlers to acknowledge the giver more often. Furthermore, there were differences in toddlers' behavior in line with these culture-specific scripts guiding gift giving. © 2016 The Authors. Child Development © 2016 Society for Research in Child Development, Inc.

  6. Using Indigenous Australian drama to break cultural barriers in healthcare relationships.

    PubMed

    Matharu, K

    2009-06-01

    Since colonisation, the marginalisation of Indigenous Australians has adversely affected their language, culture and health. Mainstream society has failed to address social differences and establish culturally-appropriate health programmes for these groups. This paper extracts important humanistic themes within the context of health from four Indigenous Australian plays written during a period of social unrest in response to past oppression: (1) The dreamers, by Jack David; (2) Murras, by Eva Johnson; (3) Coordah, by Richard Walley; and (4) The keepers, by Bob Maza. These plays will be analysed to (a) illuminate human suffering from an indigenous perspective, based upon social and cultural planes of analysis; (b) understand the socio-cultural basis of poor health; and (c) instruct healthcare professionals that health is a social construct that can be interpreted as the product of select plays that are not solely based upon an illness narrative.

  7. Response of lymphocytes to a mitogenic stimulus during spaceflight

    NASA Technical Reports Server (NTRS)

    Sonnenfeld, Gerald

    1989-01-01

    Several studies were performed that demonstrate that immunological activities of lymphocytes can be affected by spaceflight or by models that attempt to simulate some aspects of weightlessness. Included among these are the responses of lymphocytes to external stimuli such as mitogens and viruses. When cultures of lymphocytes were flown in space, the ability of the lymphocytes to respond to mitogens was inhibited. Similar results were obtained when lymphocytes from astronauts or animals just returned from space were placed into culture immediately upon return to earth, and when models of hypogravity were used. Lymphocytes placed in culture during spaceflights produced enhanced levels of interferon compared to control cultures. When cultures of lymphocytes were prepared for cosmonauts or rodents immediately upon return to earth, interferon production was inhibited. These results suggest that space flight can have profound effects on lymphocyte function, and that effects on isolated cells may be different from that on cells in the whole organism.

  8. Maternal touch and infant affect in the Still Face Paradigm: A cross-cultural examination.

    PubMed

    Lowe, Jean R; Coulombe, Patrick; Moss, Natalia C; Rieger, Rebecca E; Aragón, Crystal; MacLean, Peggy C; Caprihan, Arvind; Phillips, John P; Handal, Alexis J

    2016-08-01

    Touch between mother and infant plays an important role in development starting from birth. Cross-cultural differences surrounding rearing practices have an influence on parent-infant interaction, including types of touch used and the development of emotional regulation. This study was designed to investigate maternal touch and infant emotional regulation in infant-mother dyads from Ecuador (n=25) and Hispanic dyads from the United States (US) (n=26). Mothers and their 4-month-old full-term infants participated in the Still Face Paradigm. Second-by-second coding of maternal touch and infant affect was completed. Overall the analyses showed that Ecuadorian mothers used more nurturing and accompaniment touch and less attention seeking touch than US Hispanic mothers during the pre-stressor (baseline) episode. Lagged multilevel models were used to investigate the effect of the different types of touch on infant emotional regulation in the groups for the episodes. The data suggest that playful touch had a significant increase in infant affect, whereas accompaniment and attention-seeking touch had a significant decrease in infant affect. Overall, this study provides support for the role of touch in mother-infant synchronicity in relation to infant's emotional regulation. Identifying touch that is more calming is important to foster emotional regulation in infancy, which can have important implications for development. Published by Elsevier Inc.

  9. Impact of definition and procedures used for absent blood culture data on the rate of intravascular catheter infection during parenteral nutrition.

    PubMed

    Austin, P D; Hand, K S; Elia, M

    2016-06-01

    Diagnosis of intravascular catheter infection may be affected by the definition and procedures applied in the absence of blood culture data. To examine the extent to which different definitions of catheter infection and procedures for handling absent blood culture data can affect reported catheter infection rates. Catheter infection rates were established in a cohort of hospitalized patients administered parenteral nutrition according to three clinical and four published definitions. Paired and unpaired comparisons were made using available case analyses, sensitivity analyses and intention-to-categorize analyses. Complete data were available for each clinical definition (N = 193), and there were missing data (4.1-26.9%) for the published definitions. In an available case analysis, the catheter infection rate was 13.0-36.8% for the clinical definitions and 2.1-12.4% for the published definitions. For the published definitions, the rate was 1.6-32.1% in a sensitivity analysis and 11.4-16.9% in an intention-to-categorize analysis, with suggestion of bias towards a higher catheter infection rate in those with missing data, in keeping with the analyses of the clinical definitions. For paired comparisons, the strength of agreement between definitions varied from 'poor' (Cohen's kappa <0.21) to 'very good' (Cohen's kappa ≥0.81). The use of different definitions of catheter infection and procedures applied in the absence of blood culture data produced widely different catheter infection rates, which could compromise measurements or comparisons of service quality or study outcome. As such, there is a need to establish and use a valid, consistent and practical definition. Copyright © 2016 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.

  10. Elevated non-esterified fatty acid concentrations hamper bovine oviductal epithelial cell physiology in three different in vitro culture systems.

    PubMed

    Jordaens, L; Arias-Alvarez, M; Pintelon, I; Thys, S; Valckx, S; Dezhkam, Y; Bols, P E J; Leroy, J L M R

    2015-10-01

    Elevated non-esterified fatty acids (NEFAs) have been recognized as an important link between lipolytic metabolic conditions and impaired fertility in high-yielding dairy cows. However, NEFA effects on the oviductal micro-environment currently remain unknown. We hypothesize that elevated NEFAs may contribute to the complex pathology of subfertility by exerting a negative effect on bovine oviductal epithelial cell (BOEC) physiology. Therefore, the objectives of this study were to elucidate direct NEFA effects on BOEC physiology in three different in vitro cell culture systems. Bovine oviductal epithelial cells (four replicates) were mechanically isolated, pooled, and cultured as conventional monolayers, as explants, and in a polarized cell culture system with Dulbecco's modified Eagle's medium/F12-based culture medium. Bovine oviductal epithelial cells were exposed to an NEFA mixture of oleic, stearic, and palmitic acids for 24 hours at both physiological and pathologic concentrations. A control (0 μM NEFA) and a solvent control (0 μM NEFA + 0.45% ethanol) group were implemented. Bovine oviductal epithelial cells physiology was assessed by means of cell number and viability, a sperm binding assay, transepithelial electric resistance (TER), and a wound-healing assay. Bovine oviductal epithelial cell morphology was assessed by scanning electron microscopy on cell polarity, presence of microvilli and cilia, and monolayer integrity. Bovine oviductal epithelial cell number was negatively affected by increasing NEFAs, however, cell viability was not. Sperm binding affinity significantly decreased with increasing NEFAs and tended (P = 0.051) to be more affected by the direction of NEFA exposure in the polarized cell culture system. The absolute TER increase after NEFA exposure in the control (110 ± 11 Ω.cm(2)) was significantly higher than that in all the other treatments and was also different depending on the exposure side. Bidirectional exposed monolayers were even associated with a significant TER reduction (-15 ± 10 Ω.cm(2); P < 0.05). Cell proliferation capacity showed a decreased cell migration with increasing NEFA concentrations but was irrespective of the exposure side. Bovine oviductal epithelial cell morphology was not affected. In conclusion, in an in vitro setting, NEFAs exert a negative effect on BOEC physiology but not morphology. Ultimately, these physiological alterations in its microenvironment may result in suboptimal development of the pre-implantation embryo and a reduced reproductive outcome in dairy cattle. Copyright © 2015 Elsevier Inc. All rights reserved.

  11. Cross-cultural validity of the thyroid-specific quality-of-life patient-reported outcome measure, ThyPRO.

    PubMed

    Watt, Torquil; Barbesino, Giuseppe; Bjorner, Jakob Bue; Bonnema, Steen Joop; Bukvic, Branka; Drummond, Russell; Groenvold, Mogens; Hegedüs, Laszlo; Kantzer, Valeska; Lasch, Kathryn E; Marcocci, Claudio; Mishra, Anjali; Netea-Maier, Romana; Ekker, Merel; Paunovic, Ivan; Quinn, Terence J; Rasmussen, Åse Krogh; Russell, Audrey; Sabaretnam, Mayilvaganan; Smit, Johannes; Törring, Ove; Zivaljevic, Vladan; Feldt-Rasmussen, Ulla

    2015-03-01

    Thyroid diseases are common and often affect quality of life (QoL). No cross-culturally validated patient-reported outcome measuring thyroid-related QoL is available. The purpose of the present study was to test the cross-cultural validity of the newly developed thyroid-related patient-reported outcome ThyPRO, using tests for differential item functioning (DIF) according to language version. The ThyPRO consists of 85 items summarized in 13 multi-item scales and one single item. Scales cover physical and mental symptoms, well-being and function as well as social and daily function and cosmetic concerns. Translation applied standard forward-backward methodology with subsequent cognitive interviews and reviews. Responses (N = 1,810) to the ThyPRO were collected in seven countries: UK (n = 166), The Netherlands (n = 147), Serbia (n = 150), Italy (n = 110), India (n = 148), Denmark (n = 902) and Sweden (n = 187). Translated versions were compared pairwise to the English version by examining uniform and nonuniform DIF, i.e., whether patients from different countries respond differently to a particular item, although they have identical level of the concept measured by the item. Analyses were controlled for thyroid diagnosis. DIF was investigated by ordinal logistic regression, testing for both statistical significance and magnitude (ΔR (2) > 0.02). Scale level was estimated by the sum score, after purification. For twelve of the 84 tested items, DIF was identified in more than one language. Eight of these were small, but four were indicative of possible low translatability. Twenty-one instances of DIF in single languages were identified, indicating potential problems with the particular translation. However, only seven were of a magnitude which could affect scale scores, most of which could be explained by sample differences not controlled for. The ThyPRO has good cross-cultural validity with only minor cross-cultural invariance and is recommended for use in international multicenter studies.

  12. Lactococcus lactis Diversity in Undefined Mixed Dairy Starter Cultures as Revealed by Comparative Genome Analyses and Targeted Amplicon Sequencing of epsD.

    PubMed

    Frantzen, Cyril A; Kleppen, Hans Petter; Holo, Helge

    2018-02-01

    Undefined mesophilic mixed (DL) starter cultures are used in the production of continental cheeses and contain unknown strain mixtures of Lactococcus lactis and leuconostocs. The choice of starter culture affects the taste, aroma, and quality of the final product. To gain insight into the diversity of Lactococcus lactis strains in starter cultures, we whole-genome sequenced 95 isolates from three different starter cultures. Pan-genomic analyses, which included 30 publically available complete genomes, grouped the strains into 21 L. lactis subsp . lactis and 28 L. lactis subsp. cremoris lineages. Only one of the 95 isolates grouped with previously sequenced strains, and the three starter cultures showed no overlap in lineage distributions. The culture diversity was assessed by targeted amplicon sequencing using purR , a core gene, and epsD , present in 93 of the 95 starter culture isolates but absent in most of the reference strains. This enabled an unprecedented discrimination of starter culture Lactococcus lactis and revealed substantial differences between the three starter cultures and compositional shifts during the cultivation of cultures in milk. IMPORTANCE In contemporary cheese production, standardized frozen seed stock starter cultures are used to ensure production stability, reproducibility, and quality control of the product. The dairy industry experiences significant disruptions of cheese production due to phage attacks, and one commonly used countermeasure to phage attack is to employ a starter rotation strategy, in which two or more starters with minimal overlap in phage sensitivity are used alternately. A culture-independent analysis of the lactococcal diversity in complex undefined starter cultures revealed large differences between the three starter cultures and temporal shifts in lactococcal composition during the production of bulk starters. A better understanding of the lactococcal diversity in starter cultures will enable the development of more robust starter cultures and assist in maintaining the efficiency and stability of the production process by ensuring the presence of key bacteria that are important to the characteristics of the product. Copyright © 2018 American Society for Microbiology.

  13. Metabolic Profile of Pancreatic Acinar and Islet Tissue in Culture

    PubMed Central

    Suszynski, Thomas M.; Mueller, Kathryn; Gruessner, Angelika C.; Papas, Klearchos K.

    2016-01-01

    The amount and condition of exocrine impurities may affect the quality of islet preparations especially during culture. In this study, the objective was to determine the oxygen demandand viability of islet and acinar tissue post-isolation and whether they change disproportionately while in culture. We compare the OCR normalized to DNA (OCR/DNA, a measure of fractional viability in units nmol/min/mg DNA), and percent change in OCR and DNA recoveries between adult porcine islet and acinar tissue from the same preparation (paired) over a 6-9 days of standard culture. Paired comparisons were done to quantify differences in OCR/DNA between islet and acinar tissue from the same preparation, at specified time points during culture; the mean (± standard error) OCR/DNA was 74.0 (±11.7) units higher for acinar (vs. islet) tissue on the day of isolation (n=16, p<0.0001), but 25.7 (±9.4) units lower after 1 day (n=8, p=0.03), 56.6 (±11.5) units lower after 2 days (n=12, p=0.0004), and 65.9 (±28.7) units lower after 8 days (n=4, p=0.2) in culture. DNA and OCR recoveries decreased at different rates for acinar versus islet tissue over 6-9 days in culture (n=6). DNA recovery decreased to 24±7% for acinar and 75±8% for islets (p=0.002). Similarly, OCR recovery decreased to 16±3% for acinar and remained virtually constant for islets (p=0.005). Differences in the metabolic profile of acinarand islet tissue should be considered when culturing impure islet preparations. OCR-based measurements may help optimize pre-IT culture protocols. PMID:25131082

  14. Measuring affective temperaments: a systematic review of validation studies of the Temperament Evaluation in Memphis Pisa and San Diego (TEMPS) instruments.

    PubMed

    Elias, Liana R; Köhler, Cristiano A; Stubbs, Brendon; Maciel, Beatriz R; Cavalcante, Lígia M; Vale, Antonio M O; Gonda, Xénia; Quevedo, João; Hyphantis, Thomas N; Soares, Jair C; Vieta, Eduard; Carvalho, André F

    2017-04-01

    The assessment of affective temperaments has provided useful insights for the psychopathological understanding of affective disorders and for the conceptualization of bipolar spectrum disorders. The Temperament in Memphis Pisa and San Diego (TEMPS) instrument has been widely used in research, yet its psychometric properties and optimal factor structure are unclear. The PubMed/MEDLINE, PsycINFO, and EMBASE electronic databases were searched from inception until March 15th, 2016. Validation peer-reviewed studies of different versions of the TEMPS performed in adult samples were considered for inclusion. Twenty-seven studies (N=20,787) met inclusion criteria. Several versions of the TEMPS have been validated in 14 languages across 15 countries. The 110-item self-reported version of the TEMPS has been the most studied version. Most studies (50%) supported a five factor solution although few studies performed confirmatory factor analyses. A five-factor solution has consistently been reported for the 39-item version of the TEMPS-A. Overall, evidence indicates that different versions of the TEMPS have adequate internal consistency reliability, while the TEMPS-A-110 version has acceptable test-retest reliability. The methodological quality of included studies varied. A meta-analysis could not be performed due to the heterogeneity of settings and versions of the TEMPS utilized. Different versions of the TEMPS have been validated across different cultures. The short 39-item version of the TEMPS-A holds promise and merits further investigation. Culture-bound factors may influence the expression and/or assessment of affective temperaments with the TEMPS. Copyright © 2017 Elsevier B.V. All rights reserved.

  15. Comparison of synovial fluid culture and 16S rRNA PCR in dogs with suspected septic arthritis.

    PubMed

    Scharf, V F; Lewis, D D; Wellehan, J F; Wamsley, H L; Richardson, R

    2015-06-01

    To prospectively compare the sensitivity and specificity of 16S rRNA PCR with culture for identifying the causative organism in synovial fluid obtained from dogs with suspected septic arthritis. Synovial fluid cytology, PCR analysis and aerobic, anaerobic and Mycoplasma culture of samples from the affected joints of 18 dogs presenting with suspected septic arthritis were performed. Synovial fluid samples from the corresponding contralateral joints of 7 dogs were also analysed as negative controls. There was no significant difference between the sensitivity of bacterial detection via culture (63.2%) versus PCR (73.7%) of synovial fluid (P=0.728) or between culture and combined PCR and culture (89.5%) of synovial fluid (P=0.124). The specificity of PCR (42.9%) was significantly lower than culture specificity (100%) (P=0.07). Although 16S PCR may hold potential as an ancillary diagnostic test for identifying the causative organism in dogs with septic arthritis, our study failed to demonstrate improved accuracy compared with traditional synovial fluid culture. © 2015 Australian Veterinary Association.

  16. The role of cultural identity as a learning factor in physics: a discussion through the role of science in Brazil

    NASA Astrophysics Data System (ADS)

    Gurgel, Ivã; Pietrocola, Mauricio; Watanabe, Graciella

    2016-06-01

    In recent decades, changes in society have deeply affected the internal organization and the main goals of schools. These changes are particularly important in science education because science is one of the major sources of change in peoples' lives. This research provided the opportunity to investigate how these changes affect the way teachers develop their classroom activities. In this work, we focus on science as part of the cultural identity of a society and how this identity affects the process of teaching and learning inside the classroom. Other works have shown that certain social characteristics such as gender, race, religion, etc., can create a cultural barrier to learning science. This results in an obstacle between those particular students and the science that is taught, hindering their learning process. We first aim to present the notion of identity in education and in other related fields such as social psychology and sociology. Our main purpose is to focus on identity in a school setting and how that identity affects the relationship students have with the science content. Next, we present and analyze an intervention in the subject of Modern and Contemporary Physics composed by a sequence of activities in a private school in the region of Sao Paulo State, Brazil. This intervention serves to illustrate how scientific topics may be explored while considering aspects of cultural differences as an obstacle. The intervention was completed in two steps: first, in the classroom with a discussion concerning scientific works and nationality of scientists, with one being a Brazilian physicist; second, taking students to visit a particle collider at the University of São Paulo. One of the results of our research was realizing that students do not perceive science as something representative of the Brazilian cultural identity. At the same time, the activity gave the students the opportunity to make the connection between doing physical sciences at an international level and the national level in Brazil. The findings of this study suggest that it is possible to reshape the cultural identity of Brazilian students.

  17. In vitro development rate of preimplantation rabbit embryos cultured with different levels of melatonin.

    PubMed

    Mehaisen, Gamal Mohamed Kamel; Saeed, Ayman Moustafa

    2015-02-01

    This study aimed to investigate the effect of melatonin supplementation at different levels in culture medium on embryo development in rabbits. Embryos of 2-4 cells, 8-16 cells and morula stages were recovered from nulliparous Red Baladi rabbit does by laparotomy technique 24, 48 and 72 h post-insemination, respectively. Normal embryos from each stage were cultured to hatched blastocyst stages in either control culture medium (TCM-199 + 20% fetal bovine serum) or control supplemented with melatonin at 10(-3) M, 10(-6) M or 10(-9) M. No effect of melatonin was found on development of embryos recovered at 24 h post-insemination. The high level of melatonin at 10(-3) M adversely affected the in vitro development rates of embryos recovered at 48 h post-insemination (52 versus 86, 87 and 80% blastocyst rate; 28 versus 66, 78 and 59% hatchability rate for 10(-3) M versus 10(-9) M, 10(-6) M and control, respectively, P< 0.05). At the morula stage, melatonin at 10-3 M significantly increased the in vitro development of embryos (92% for 10(-3) M versus 76% for control, P < 0.05), while the hatchability rate of these embryos was not improved by melatonin (16-30% versus 52% for melatonin groups versus control, P < 0.05). Results show that a moderate level of melatonin (10(-6) M) may improve the development and hatchability rates of preimplantation rabbit embryos. The addition of melatonin at a 10-3 M concentration enhances the development of rabbit morulae but may negatively affect the development of earlier embryos. More studies are needed to optimize the use of melatonin in in vitro embryo culture in rabbits.

  18. Altered physiological functions and ion currents in atrial fibroblasts from patients with chronic atrial fibrillation.

    PubMed

    Poulet, Claire; Künzel, Stephan; Büttner, Edgar; Lindner, Diana; Westermann, Dirk; Ravens, Ursula

    2016-02-01

    The contribution of human atrial fibroblasts to cardiac physiology and pathophysiology is poorly understood. Fibroblasts may contribute to arrhythmogenesis through fibrosis, or by directly altering electrical activity in cardiomyocytes. The objective of our study was to uncover phenotypic differences between cells from patients in sinus rhythm (SR) and chronic atrial fibrillation (AF), with special emphasis on electrophysiological properties. We isolated fibroblasts from human right atrial tissue for patch-clamp experiments, proliferation, migration, and differentiation assays, and gene expression profiling. In culture, proliferation and migration of AF fibroblasts were strongly impaired but differentiation into myofibroblasts was increased. This was associated with a higher number of AF fibroblasts expressing functional Nav1.5 channels. Strikingly Na(+) currents were considerably larger in AF cells. Blocking Na(+) channels in culture with tetrodotoxin did not affect proliferation, migration, or differentiation in neither SR nor AF cells. While freshly isolated fibroblasts showed mostly weak rectifier currents, fibroblasts in culture developed outward rectifier K(+) currents of similar amplitude between the SR and AF groups. Adding the K(+) channel blockers tetraethylammonium and 4-aminopyridin in culture reduced current amplitude and inhibited proliferation in the SR group only. Analysis of gene expression revealed significant differences between SR and AF in genes encoding for ion channels, collagen, growth factors, connexins, and cadherins. In conclusion, this study shows that under AF conditions atrial fibroblasts undergo phenotypic changes that are revealed in culture. Future experiments should be performed in situ to understand the nature of those changes and whether they affect cardiac electrical activity. © 2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

  19. How do national cultures influence lay people's preferences toward doctors' style of communication? A comparison of 35 focus groups from an European cross national research.

    PubMed

    Rimondini, Michela; Mazzi, Maria Angela; Deveugele, Myriam; Bensing, Jozien M

    2015-12-14

    The evidence that inspires and fosters communication skills, teaching programmes and clinical recommendations are often based on national studies which assume, implicitly, that patients' preferences towards doctors' communication style are not significantly affected by their cultural background. The cross-cultural validity of national results has been recognized as a potential limitation on how generally applicable they are in a wider context. Using 35 country-specific focus group discussions from four European countries, the aim of the present study is to test whether or not national cultures influence lay people's preferences towards doctors' style of communication. Lay people preferences on doctor's communication style have been collected in Belgium, the Netherlands, the United Kingdom and Italy. Each centre organized between eight and nine focus groups, where participants (n = 259) were asked to comment on a video of a simulated medical interview. The discussions were audiotaped, transcribed and coded using a common framework (Guliver Coding System) that allowed for the identification of different themes. The frequency distribution of the topics discussed highlights lay people's generally positive views towards most part of doctors interventions. The regression model applied to the Guliver categories highlighted slight national differences and the existence of a cross-cultural appreciation, in particular, of five types of intervention: Doctors attitudes (both Task-Oriented and Affective/Emotional), Summarizing, Structuring and Providing solution. Lay panels valued doctors' communication style in a similar manner in the countries selected. This highlights the existence of a common background, which in the process of internationalization of heath care, might foster the implementation of cross-national teaching programmes and clinical guidelines.

  20. Toxicity of Functional Nano-Micro Zinc Oxide Tetrapods: Impact of Cell Culture Conditions, Cellular Age and Material Properties

    PubMed Central

    Papavlassopoulos, Heike; Mishra, Yogendra K.; Kaps, Sören; Paulowicz, Ingo; Abdelaziz, Ramzy; Elbahri, Mady; Maser, Edmund; Adelung, Rainer; Röhl, Claudia

    2014-01-01

    With increasing production and applications of nanostructured zinc oxide, e.g., for biomedical and consumer products, the question of safety is getting more and more important. Different morphologies of zinc oxide structures have been synthesized and accordingly investigated. In this study, we have particularly focused on nano-micro ZnO tetrapods (ZnO-T), because their large scale fabrication has been made possible by a newly introduced flame transport synthesis approach which will probably lead to several new applications. Moreover, ZnO-T provide a completely different morphology then classical spherical ZnO nanoparticles. To get a better understanding of parameters that affect the interactions between ZnO-T and mammalian cells, and thus their biocompatibility, we have examined the impact of cell culture conditions as well as of material properties on cytotoxicity. Our results demonstrate that the cell density of fibroblasts in culture along with their age, i.e., the number of preceding cell divisions, strongly affect the cytotoxic potency of ZnO-T. Concerning the material properties, the toxic potency of ZnO-T is found to be significantly lower than that of spherical ZnO nanoparticles. Furthermore, the morphology of the ZnO-T influenced cellular toxicity in contrast to surface charges modified by UV illumination or O2 treatment and to the material age. Finally, we have observed that direct contact between tetrapods and cells increases their toxicity compared to transwell culture models which allow only an indirect effect via released zinc ions. The results reveal several parameters that can be of importance for the assessment of ZnO-T toxicity in cell cultures and for particle development. PMID:24454775

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