The eddy current probe array for Keda Torus eXperiment.

PubMed

Li, Zichao; Li, Hong; Tu, Cui; Hu, Jintong; You, Wei; Luo, Bing; Tan, Mingsheng; Adil, Yolbarsop; Wu, Yanqi; Shen, Biao; Xiao, Bingjia; Zhang, Ping; Mao, Wenzhe; Wang, Hai; Wen, Xiaohui; Zhou, Haiyang; Xie, Jinlin; Lan, Tao; Liu, Adi; Ding, Weixing; Xiao, Chijin; Liu, Wandong

2016-11-01

In a reversed field pinch device, the conductive shell is placed as close as possible to the plasma so as to balance the plasma during discharge. Plasma instabilities such as the resistive wall mode and certain tearing modes, which restrain the plasma high parameter operation, respond closely with conditions in the wall, in essence the eddy current present. Also, the effect of eddy currents induced by the external coils cannot be ignored when active control is applied to control instabilities. One diagnostic tool, an eddy current probe array, detects the eddy current in the composite shell. Magnetic probes measuring differences between the inner and outer magnetic fields enable estimates of the amplitude and angle of these eddy currents. Along with measurements of currents through the copper bolts connecting the poloidal shield copper shells, we can obtain the eddy currents over the entire shell. Magnetic field and eddy current resolutions approach 2 G and 6 A, respectively. Additionally, the vortex electric field can be obtained by eddy current probes. As the conductivity of the composite shell is high, the eddy current probe array is very sensitive to the electric field and has a resolution of 0.2 mV/cm. In a bench test experiment using a 1/4 vacuum vessel, measurements of the induced eddy currents are compared with simulation results based on a 3D electromagnetic model. The preliminary data of the eddy currents have been detected during discharges in a Keda Torus eXperiment device. The typical value of toroidal and poloidal eddy currents across the magnetic probe coverage rectangular area could reach 3.0 kA and 1.3 kA, respectively.

Protein recognition by a pattern-generating fluorescent molecular probe.

PubMed

Pode, Zohar; Peri-Naor, Ronny; Georgeson, Joseph M; Ilani, Tal; Kiss, Vladimir; Unger, Tamar; Markus, Barak; Barr, Haim M; Motiei, Leila; Margulies, David

2017-12-01

Fluorescent molecular probes have become valuable tools in protein research; however, the current methods for using these probes are less suitable for analysing specific populations of proteins in their native environment. In this study, we address this gap by developing a unimolecular fluorescent probe that combines the properties of small-molecule-based probes and cross-reactive sensor arrays (the so-called chemical 'noses/tongues'). On the one hand, the probe can detect different proteins by generating unique identification (ID) patterns, akin to cross-reactive arrays. On the other hand, its unimolecular scaffold and selective binding enable this ID-generating probe to identify combinations of specific protein families within complex mixtures and to discriminate among isoforms in living cells, where macroscopic arrays cannot access. The ability to recycle the molecular device and use it to track several binding interactions simultaneously further demonstrates how this approach could expand the fluorescent toolbox currently used to detect and image proteins.

Protein recognition by a pattern-generating fluorescent molecular probe

NASA Astrophysics Data System (ADS)

Pode, Zohar; Peri-Naor, Ronny; Georgeson, Joseph M.; Ilani, Tal; Kiss, Vladimir; Unger, Tamar; Markus, Barak; Barr, Haim M.; Motiei, Leila; Margulies, David

2017-12-01

Fluorescent molecular probes have become valuable tools in protein research; however, the current methods for using these probes are less suitable for analysing specific populations of proteins in their native environment. In this study, we address this gap by developing a unimolecular fluorescent probe that combines the properties of small-molecule-based probes and cross-reactive sensor arrays (the so-called chemical 'noses/tongues'). On the one hand, the probe can detect different proteins by generating unique identification (ID) patterns, akin to cross-reactive arrays. On the other hand, its unimolecular scaffold and selective binding enable this ID-generating probe to identify combinations of specific protein families within complex mixtures and to discriminate among isoforms in living cells, where macroscopic arrays cannot access. The ability to recycle the molecular device and use it to track several binding interactions simultaneously further demonstrates how this approach could expand the fluorescent toolbox currently used to detect and image proteins.

Probing of multiple magnetic responses in magnetic inductors using atomic force microscopy.

PubMed

Park, Seongjae; Seo, Hosung; Seol, Daehee; Yoon, Young-Hwan; Kim, Mi Yang; Kim, Yunseok

2016-02-08

Even though nanoscale analysis of magnetic properties is of significant interest, probing methods are relatively less developed compared to the significance of the technique, which has multiple potential applications. Here, we demonstrate an approach for probing various magnetic properties associated with eddy current, coil current and magnetic domains in magnetic inductors using multidimensional magnetic force microscopy (MMFM). The MMFM images provide combined magnetic responses from the three different origins, however, each contribution to the MMFM response can be differentiated through analysis based on the bias dependence of the response. In particular, the bias dependent MMFM images show locally different eddy current behavior with values dependent on the type of materials that comprise the MI. This approach for probing magnetic responses can be further extended to the analysis of local physical features.

Label-free biosensing with functionalized nanopipette probes

PubMed Central

Umehara, Senkei; Karhanek, Miloslav; Davis, Ronald W.; Pourmand, Nader

2009-01-01

Nanopipette technology can uniquely identify biomolecules such as proteins based on differences in size, shape, and electrical charge. These differences are determined by the detection of changes in ionic current as the proteins interact with the nanopipette tip coated with probe molecules. Here we show that electrostatic, biotin-streptavidin, and antibody-antigen interactions on the nanopipette tip surface affect ionic current flowing through a 50-nm pore. Highly charged polymers interacting with the glass surface modulated the rectification property of the nanopipette electrode. Affinity-based binding between the probes tethered to the surface and their target proteins caused a change in the ionic current due to a partial blockade or an altered surface charge. These findings suggest that nanopipettes functionalized with appropriate molecular recognition elements can be used as nanosensors in biomedical and biological research. PMID:19264962

Method and apparatus for remote tube crevice detection by current and voltage probe resistance measurement

DOEpatents

Kikta, Thomas J.; Mitchell, Ronald D.

1992-01-01

A method and apparatus for determining the extent of contact between an electrically conducting tube and an electrically conductive tubesheet surrounding the tube, based upon the electrical resistance of the tube and tubesheet. A constant current source is applied to the interior of the electrically conducting tube by probes and a voltmeter is connected between other probes to measure the voltage at the point of current injection, which is inversely proportional to the amount of contact between the tube and tubesheet. Namely, the higher the voltage measured by the voltmeter, the less contact between the tube and tubesheet.

Method and apparatus for remote tube crevice detection by current and voltage probe resistance measurement

DOEpatents

Kikta, T.J.; Mitchell, R.D.

1992-11-24

A method and apparatus for determining the extent of contact between an electrically conducting tube and an electrically conductive tubesheet surrounding the tube, based upon the electrical resistance of the tube and tubesheet. A constant current source is applied to the interior of the electrically conducting tube by probes and a voltmeter is connected between other probes to measure the voltage at the point of current injection, which is inversely proportional to the amount of contact between the tube and tubesheet. Namely, the higher the voltage measured by the voltmeter, the less contact between the tube and tubesheet. 4 figs.

Electron density and electron temperature measurement in a bi-Maxwellian electron distribution using a derivative method of Langmuir probes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Choi, Ikjin; Chung, ChinWook; Youn Moon, Se

2013-08-15

In plasma diagnostics with a single Langmuir probe, the electron temperature T{sub e} is usually obtained from the slope of the logarithm of the electron current or from the electron energy probability functions of current (I)-voltage (V) curve. Recently, Chen [F. F. Chen, Phys. Plasmas 8, 3029 (2001)] suggested a derivative analysis method to obtain T{sub e} by the ratio between the probe current and the derivative of the probe current at a plasma potential where the ion current becomes zero. Based on this method, electron temperatures and electron densities were measured and compared with those from the electron energymore » distribution function (EEDF) measurement in Maxwellian and bi-Maxwellian electron distribution conditions. In a bi-Maxwellian electron distribution, we found the electron temperature T{sub e} obtained from the method is always lower than the effective temperatures T{sub eff} derived from EEDFs. The theoretical analysis for this is presented.« less

One-by-one single-molecule detection of mutated nucleobases by monitoring tunneling current using a DNA tip.

PubMed

Bui, Phuc Tan; Nishino, Tomoaki; Shiigi, Hiroshi; Nagaoka, Tsutomu

2015-01-31

A DNA molecule was utilized as a probe tip to achieve single-molecule genetic diagnoses. Hybridization of the probe and target DNAs resulted in electron tunneling along the emergent double-stranded DNA. Simple stationary monitoring of the tunneling current leads to single-molecule DNA detection and discovery of base mismatches and methylation.

Analysis of Eddy Current Capabilities for the Detection of Outer Diameter Stress Corrosion Cracking in Small Bore Metallic Structures

NASA Technical Reports Server (NTRS)

Wincheski, Buzz; Williams, Phillip; Simpson, John

2007-01-01

The use of eddy current techniques for the detection of outer diameter damage in tubing and many complex aerospace structures often requires the use of an inner diameter probe due to a lack of access to the outside of the part. In small bore structures the probe size and orientation are constrained by the inner diameter of the part, complicating the optimization of the inspection technique. Detection of flaws through a significant remaining wall thickness becomes limited not only by the standard depth of penetration, but also geometrical aspects of the probe. Recently, an orthogonal eddy current probe was developed for detection of such flaws in Space Shuttle Primary Reaction Control System (PRCS) Thrusters. In this case, the detection of deeply buried stress corrosion cracking by an inner diameter eddy current probe was sought. Probe optimization was performed based upon the limiting spatial dimensions, flaw orientation, and required detection sensitivity. Analysis of the probe/flaw interaction was performed through the use of finite and boundary element modeling techniques. Experimental data for the flaw detection capabilities, including a probability of detection study, will be presented along with the simulation data. The results of this work have led to the successful deployment of an inspection system for the detection of stress corrosion cracking in Space Shuttle Primary Reaction Control System (PRCS) Thrusters.

Development of DNA biosensor based on TiO2 nanoparticles

NASA Astrophysics Data System (ADS)

Nadzirah, Sh.; Hashim, U.; Rusop, M.

2018-05-01

A novel technique of DNA hybridization on the TiO2 nanoparticles film was developed by dropping a single droplet of target DNA onto the surface of TiO2 for the study of various concentrations of target DNA. The surface of TiO2 nanoparticle film was functionalized with APTES and covalently immobilized with 1 µM probe DNA on the silanized TiO2 nanoparticles surface. The effect of silanization, immobilization and hybridization were quantitatively measured by the output current signal obtained using a picoammeter. The 1 µM target DNA was found to be the most effective target towards the 1 µM probe DNA as the output current signal was within range; while the output current signal of the 10 µM target DNA was observed to beyond the range of the probe DNA control due to the excessive concentration as compared to the probe DNA. This approach has several advantages such as rapid, simple, low cost, and sensitive current signal during detection of different target DNA concentrations.

Electrochemical immobilization of Fluorescent labelled probe molecules on a FTO surface for affinity detection based on photo-excited current

NASA Astrophysics Data System (ADS)

Haruyama, Tetsuya; Wakabayashi, Ryo; Cho, Takeshi; Matsuyama, Sho-taro

2011-10-01

Photo-excited current can be generated at a molecular interface between a photo-excited molecules and a semi-conductive material in appropriate condition. The system has been recognized for promoting photo-energy devices such as an organic dye sensitized solar-cell. The photo-current generated reactions are totally dependent on the interfacial energy reactions, which are in a highly fluctuated interfacial environment. The authors investigated the photo-excited current reaction to develop a smart affinity detection method. However, in order to perform both an affinity reaction and a photo-excited current reaction at a molecular interface, ordered fabrications of the functional (affinity, photo-excitation, etc.) molecules layer on a semi-conductive surface is required. In the present research, we would like to present the fabrication and functional performance of photo-excited current-based affinity assay device and its application for detection of endocrine disrupting chemicals. On the FTO surface, fluorescent pigment labelled affinity peptide was immobilized through the EC tag (electrochemical-tag) method. The modified FTO produced a current when it was irradiated with diode laser light. However, the photo current decreased drastically when estrogen (ES) coexisted in the reaction solution. In this case, immobilized affinity probe molecules formed a complex with ES and estrogen receptor (ER). The result strongly suggests that the photo-excited current transduction between probe molecule-labelled cyanine pigment and the FTO surface was partly inhibited by a complex that formed at the affinity oligo-peptide region in a probe molecule on the FTO electrode. The bound bulky complex may act as an impediment to perform smooth transduction of photo-excited current in the molecular interface. The present system is new type of photo-reaction-based analysis. This system can be used to perform simple high-sensitive homogeneous assays.

Determining Confounding Sensitivities In Eddy Current Thin Film Measurements

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gros, Ethan; Udpa, Lalita; Smith, James A.

Determining Confounding Sensitivities In Eddy Current Thin Film Measurements Ethan Gros, Lalita Udpa, Electrical Engineering, Michigan State University, East Lansing MI 48824 James A. Smith, Experiment Analysis, Idaho National Laboratory, Idaho Falls ID 83415 Eddy current (EC) techniques are widely used in industry to measure the thickness of non-conductive films on a metal substrate. This is done using a system whereby a coil carrying a high-frequency alternating current is used to create an alternating magnetic field at the surface of the instrument's probe. When the probe is brought near a conductive surface, the alternating magnetic field will induce ECs inmore » the conductor. The substrate characteristics and the distance of the probe from the substrate (the coating thickness) affect the magnitude of the ECs. The induced currents load the probe coil affecting the terminal impedance of the coil. The measured probe impedance is related to the lift off between coil and conductor as well as conductivity of the test sample. For a known conductivity sample, the probe impedance can be converted into an equivalent film thickness value. The EC measurement can be confounded by a number of measurement parameters. It is the goal of this research to determine which physical properties of the measurement set-up and sample can adversely affect the thickness measurement. The eddy current testing is performed using a commercially available, hand held eddy current probe (ETA3.3H spring loaded eddy probe running at 8 MHz) that comes with a stand to hold the probe. The stand holds the probe and adjusts the probe on the z-axis to help position the probe in the correct area as well as make precise measurements. The signal from the probe is sent to a hand held readout, where the results are recorded directly in terms of liftoff or film thickness. Understanding the effect of certain factors on the measurements of film thickness, will help to evaluate how accurate the ETA3.3H spring loaded eddy probe is at measuring film thickness under varying experimental conditions. This research will study the effects of a number of factors such as i) calibration, ii) conductivity, iii) edge effect, iv) surface finish of base material and v) cable condition and compare with the long term reproducibility of a standard measurement. This work was performed with support from the Department of Energy under the United States National Nuclear Security Administration (NNSA) at the Idaho National Laboratory.« less

Plasma properties and heating at the anode of a 1 kW arcjet using electrostatic probes

NASA Astrophysics Data System (ADS)

Tiliakos, Nicholas

A 1 kW hydrazine arcjet thruster has been modified for internal probing of the near-anode boundary layer with an array of fourteen electrostatic micro-probes. The main objectives of this experimental investigation were to: (1) obtain axial and azimuthal distributions of floating potential phisbf, anode sheath potential phisbs, probe current density at zero volts jsba, electron number density nsbes, electron temperature Tsbes, and anode heating due to electrons qsbe for arc currents Isbarc, between 7.8 and 10.6 A, propellant flow rates m = 40-60 mg/s, and specific energies, 18.8 MJ/kg ≤ P/m ≤ 27.4 MJ/kg; (2) probe the anode boundary layer using flush-mounted and cylindrical micro-probes; (3) verify azimuthal current symmetry; (4) understand what affects anode heating, a critical thruster lifetime issue; and (5) provide experimental data for validation of the Megli-Krier-Burton (MKB) model. All of the above objectives were met through the design, fabrication and implementation of fourteen electrostatic micro-probes, of sizes ranging from 0.170 mm to 0.43 mm in diameter. A technique for cleaning and implementing these probes was developed. Two configurations were used: flush-mounted planar probes and cylindrical probes extended 0.10-0.30 mm into the plasma flow. The main results of this investigation are: (1) electrostatic micro-probes can successfully be used in the harsh environment of an arcjet; (2) under all conditions tested the plasma is highly non-equilibrium in the near-anode region; (3) azimuthal current symmetry exists for most operating conditions; (4) the propellant flow rate affects the location of maximum anode sheath potential, current density, and anode heating more than the arc current; (5) the weighted anode sheath potential is always positive and varies from 8-17 V depending on thruster operating conditions; (6) the fraction of anode heating varies from 18-24% of the total input power over the range of specific energies tested; and (7) based on an energy loss factor of delta = 1200, reasonable correlation between the experimental data and the MKB model was found.

Current-Voltage and Floating-Potential characteristics of cylindrical emissive probes from a full-kinetic model based on the orbital motion theory

NASA Astrophysics Data System (ADS)

Chen, Xin; Sánchez-Arriaga, Gonzalo

2018-02-01

To model the sheath structure around an emissive probe with cylindrical geometry, the Orbital-Motion theory takes advantage of three conserved quantities (distribution function, transverse energy, and angular momentum) to transform the stationary Vlasov-Poisson system into a single integro-differential equation. For a stationary collisionless unmagnetized plasma, this equation describes self-consistently the probe characteristics. By solving such an equation numerically, parametric analyses for the current-voltage (IV) and floating-potential (FP) characteristics can be performed, which show that: (a) for strong emission, the space-charge effects increase with probe radius; (b) the probe can float at a positive potential relative to the plasma; (c) a smaller probe radius is preferred for the FP method to determine the plasma potential; (d) the work function of the emitting material and the plasma-ion properties do not influence the reliability of the floating-potential method. Analytical analysis demonstrates that the inflection point of an IV curve for non-emitting probes occurs at the plasma potential. The flat potential is not a self-consistent solution for emissive probes.

First-Year University Physics Students' Knowledge about Direct Current Circuits: Probing Improvement in Understanding as a Function of Teaching and Learning Interventions

ERIC Educational Resources Information Center

Newman, Richard; van der Ventel, Brandon; Hanekom, Crischelle

2017-01-01

Probing university students' understanding of direct-current (DC) resistive circuits is still a field of active physics education research. We report here on a study we conducted of this understanding, where the cohort consisted of students in a large-enrollment first-year physics module. This is a non-calculus based physics module for students in…

An Effective Electrical Resonance-Based Method to Detect Delamination in Thermal Barrier Coating

NASA Astrophysics Data System (ADS)

Kim, Jong Min; Park, Jae-Ha; Lee, Ho Girl; Kim, Hak-Joon; Song, Sung-Jin; Seok, Chang-Sung; Lee, Young-Ze

2017-12-01

This research proposes a simple yet highly sensitive method based on electrical resonance of an eddy-current probe to detect delamination of thermal barrier coating (TBC). This method can directly measure the mechanical characteristics of TBC compared to conventional ultrasonic testing and infrared thermography methods. The electrical resonance-based method can detect the delamination of TBC from the metallic bond coat by shifting the electrical impedance of eddy current testing (ECT) probe coupling with degraded TBC, and, due to this shift, the resonant frequencies near the peak impedance of ECT probe revealed high sensitivity to the delamination. In order to verify the performance of the proposed method, a simple experiment is performed with degraded TBC specimens by thermal cyclic exposure. Consequently, the delamination with growth of thermally grown oxide in a TBC system is experimentally identified. Additionally, the results are in good agreement with the results obtained from ultrasonic C-scanning.

An Effective Electrical Resonance-Based Method to Detect Delamination in Thermal Barrier Coating

NASA Astrophysics Data System (ADS)

Kim, Jong Min; Park, Jae-Ha; Lee, Ho Girl; Kim, Hak-Joon; Song, Sung-Jin; Seok, Chang-Sung; Lee, Young-Ze

2018-02-01

This research proposes a simple yet highly sensitive method based on electrical resonance of an eddy-current probe to detect delamination of thermal barrier coating (TBC). This method can directly measure the mechanical characteristics of TBC compared to conventional ultrasonic testing and infrared thermography methods. The electrical resonance-based method can detect the delamination of TBC from the metallic bond coat by shifting the electrical impedance of eddy current testing (ECT) probe coupling with degraded TBC, and, due to this shift, the resonant frequencies near the peak impedance of ECT probe revealed high sensitivity to the delamination. In order to verify the performance of the proposed method, a simple experiment is performed with degraded TBC specimens by thermal cyclic exposure. Consequently, the delamination with growth of thermally grown oxide in a TBC system is experimentally identified. Additionally, the results are in good agreement with the results obtained from ultrasonic C-scanning.

A "signal on" protection-displacement-hybridization-based electrochemical hepatitis B virus gene sequence sensor with high sensitivity and peculiar adjustable specificity.

PubMed

Li, Fengqin; Xu, Yanmei; Yu, Xiang; Yu, Zhigang; He, Xunjun; Ji, Hongrui; Dong, Jinghao; Song, Yongbin; Yan, Hong; Zhang, Guiling

2016-08-15

One "signal on" electrochemical sensing strategy was constructed for the detection of a specific hepatitis B virus (HBV) gene sequence based on the protection-displacement-hybridization-based (PDHB) signaling mechanism. This sensing system is composed of three probes, one capturing probe (CP) and one assistant probe (AP) which are co-immobilized on the Au electrode surface, and one 3-methylene blue (MB) modified signaling probe (SP) free in the detection solution. One duplex are formed between AP and SP with the target, a specific HBV gene sequence, hybridizing with CP. This structure can drive the MB labels close to the electrode surface, thereby producing a large detection current. Two electrochemical testing techniques, alternating current voltammetry (ACV) and cyclic voltammetry (CV), were used for characterizing the sensor. Under the optimized conditions, the proposed sensor exhibits a high sensitivity with the detection limit of ∼5fM for the target. When used for the discrimination of point mutation, the sensor also features an outstanding ability and its peculiar high adjustability. Copyright © 2016 Elsevier B.V. All rights reserved.

The design and application of fluorophore–gold nanoparticle activatable probes

PubMed Central

Swierczewska, Magdalena; Lee, Seulki; Chen, Xiaoyuan

2013-01-01

Fluorescence-based assays and detection techniques are among the most highly sensitive and popular biological tests for researchers. To match the needs of research and the clinic, detection limits and specificities need to improve, however. One mechanism is to decrease non-specific background signals, which is most efficiently done by increasing fluorescence quenching abilities. Reports in the literature of theoretical and experimental work have shown that metallic gold surfaces and nanoparticles are ultra-efficient fluorescence quenchers. Based on these findings, subsequent reports have described gold nanoparticle fluorescence-based activatable probes that were designed to increase fluorescence intensity based on a range of stimuli. In this way, these probes can detect and signify assorted biomarkers and changes in environmental conditions. In this review, we explore the various factors and theoretical models that affect gold nanoparticle fluorescence quenching, explore current uses of activatable probes, and propose an engineering approach for future development of fluorescence based gold nanoparticle activatable probes. PMID:21380462

Electrical probe characteristic recovery by measuring only one time-dependent parameter

NASA Astrophysics Data System (ADS)

Costin, C.; Popa, G.; Anita, V.

2016-03-01

Two straightforward methods for recovering the current-voltage characteristic of an electrical probe are proposed. Basically, they consist of replacing the usual power supply from the probe circuit with a capacitor which can be charged or discharged by the probe current drained from the plasma. The experiment requires the registration of only one time-dependent electrical parameter, either the probe current or the probe voltage. The corresponding time-dependence of the second parameter, the probe voltage, or the probe current, respectively, can be calculated using an integral or a differential relation and the current-voltage characteristic of the probe can be obtained.

A computerized Langmuir probe system

NASA Astrophysics Data System (ADS)

Pilling, L. S.; Bydder, E. L.; Carnegie, D. A.

2003-07-01

For low pressure plasmas it is important to record entire single or double Langmuir probe characteristics accurately. For plasmas with a depleted high energy tail, the accuracy of the recorded ion current plays a critical role in determining the electron temperature. Even for high density Maxwellian distributions, it is necessary to accurately model the ion current to obtain the correct electron density. Since the electron and ion current saturation values are, at best, orders of magnitude apart, a single current sensing resistor cannot provide the required resolution to accurately record these values. We present an automated, personal computer based data acquisition system for the determination of fundamental plasma properties in low pressure plasmas. The system is designed for single and double Langmuir probes, whose characteristics can be recorded over a bias voltage range of ±70 V with 12 bit resolution. The current flowing through the probes can be recorded within the range of 5 nA-100 mA. The use of a transimpedance amplifier for current sensing eliminates the requirement for traditional current sensing resistors and hence the need to correct the raw data. The large current recording range is realized through the use of a real time gain switching system in the negative feedback loop of the transimpedance amplifier.

Performance measures to characterize directional corridor travel time delay based on probe vehicle data : final report.

DOT National Transportation Integrated Search

2015-10-01

Anonymous probe vehicle data are currently being collected on roadways throughout the United States. These data are being incorporated into local and statewide mobility reports to measure the performance of freeways and arterial systems. Predefined s...

Probing condensed matter physics with magnetometry based on nitrogen-vacancy centres in diamond

NASA Astrophysics Data System (ADS)

Casola, Francesco; van der Sar, Toeno; Yacoby, Amir

2018-01-01

The magnetic fields generated by spins and currents provide a unique window into the physics of correlated-electron materials and devices. First proposed only a decade ago, magnetometry based on the electron spin of nitrogen-vacancy (NV) defects in diamond is emerging as a platform that is excellently suited for probing condensed matter systems; it can be operated from cryogenic temperatures to above room temperature, has a dynamic range spanning from direct current to gigahertz and allows sensor-sample distances as small as a few nanometres. As such, NV magnetometry provides access to static and dynamic magnetic and electronic phenomena with nanoscale spatial resolution. Pioneering work has focused on proof-of-principle demonstrations of its nanoscale imaging resolution and magnetic field sensitivity. Now, experiments are starting to probe the correlated-electron physics of magnets and superconductors and to explore the current distributions in low-dimensional materials. In this Review, we discuss the application of NV magnetometry to the exploration of condensed matter physics, focusing on its use to study static and dynamic magnetic textures and static and dynamic current distributions.

Non-toxic fluorescent phosphonium probes to detect mitochondrial potential.

PubMed

Šarić, Ana; Crnolatac, Ivo; Bouillaud, Frédéric; Sobočanec, Sandra; Mikecin, Ana-Matea; Mačak Šafranko, Željka; Delgeorgiev, Todor; Piantanida, Ivo; Balog, Tihomir; Petit, Patrice X

2017-03-22

We evaluated our phosphonium-based fluorescent probes for selective staining of mitochondria. Currently used probes for monitoring mitochondrial membrane potential show varying degrees of interference with cell metabolism, photo-induced damage and probe binding. Here presented probes are characterised by highly efficient cellular uptake and specific accumulation in mitochondria. Fluorescent detection of the probes was accomplished using flow cytometry and confocal microscopy imaging of yeast and mammalian cells. Toxicity analysis (impedimetry-xCELLigence for the cellular proliferation and Seahorse technology for respiratory properties) confirms that these dyes exhibit no-toxicity on mitochondrial or cellular functioning even for long time incubation. The excellent chemical and photophysical stability of the dyes makes them promising leads toward improved fluorescent probes. Therefore, the probes described here offer to circumvent the problems associated with existing-probe's limitations.

Natural Crack Sizing Based on Eddy Current Image and Electromagnetic Field Analyses

DOE Office of Scientific and Technical Information (OSTI.GOV)

Endo, H.; Uchimoto, T.; Takagi, T.

2006-03-06

An eddy current testing (ECT) system with multi-coil type probes is applied to size up cracks fabricated on austenite stainless plates. We have developed muti-channel ECT system to produce data as digital images. The probes consist of transmit-receive type sensors as elements to classify crack directions, working as two scan direction modes simultaneously. Template matching applied to the ECT images determines regions of interest in sizing up cracks. Finite element based inversion sizes up the crack depth from the measured ECT signal. The present paper demonstrates this approach for fatigue crack and stress corrosion cracking.

Outer planet probe cost estimates: First impressions

NASA Technical Reports Server (NTRS)

Niehoff, J.

1974-01-01

An examination was made of early estimates of outer planetary atmospheric probe cost by comparing the estimates with past planetary projects. Of particular interest is identification of project elements which are likely cost drivers for future probe missions. Data are divided into two parts: first, the description of a cost model developed by SAI for the Planetary Programs Office of NASA, and second, use of this model and its data base to evaluate estimates of probe costs. Several observations are offered in conclusion regarding the credibility of current estimates and specific areas of the outer planet probe concept most vulnerable to cost escalation.

Eddy Current Sensing of Torque in Rotating Shafts

NASA Astrophysics Data System (ADS)

Varonis, Orestes J.; Ida, Nathan

2013-12-01

The noncontact torque sensing in machine shafts is addressed based on the stress induced in a press-fitted magnetoelastic sleeve on the shaft and eddy current sensing of the changes of electrical conductivity and magnetic permeability due to the presence of stress. The eddy current probe uses dual drive, dual sensing coils whose purpose is increased sensitivity to torque and decreased sensitivity to variations in distance between probe and shaft (liftoff). A mechanism of keeping the distance constant is also employed. Both the probe and the magnetoelastic sleeve are evaluated for performance using a standard eddy current instrument. An eddy current instrument is also used to drive the coils and analyze the torque data. The method and sensor described are general and adaptable to a variety of applications. The sensor is suitable for static and rotating shafts, is independent of shaft diameter and operational over a large range of torques. The torque sensor uses a differential eddy current measurement resulting in cancellation of common mode effects including temperature and vibrations.

Fluorescent probes for lipid rafts: from model membranes to living cells.

PubMed

Klymchenko, Andrey S; Kreder, Rémy

2014-01-16

Membrane microdomains (rafts) remain one of the controversial issues in biophysics. Fluorescent molecular probes, which make these lipid nanostructures visible through optical techniques, are one of the tools currently used to study lipid rafts. The most common are lipophilic fluorescent probes that partition specifically into liquid ordered or liquid disordered phase. Their partition depends on the lipid composition of a given phase, which complicates their use in cellular membranes. A second class of probes is based on environment-sensitive dyes, which partition into both phases, but stain them by different fluorescence color, intensity, or lifetime. These probes can directly address the properties of each separate phase, but their cellular applications are still limited. The present review focuses on summarizing the current state in the field of developing and applying fluorescent molecular probes to study lipid rafts. We highlight an urgent need to develop new probes, specifically adapted for cell plasma membranes and compatible with modern fluorescence microscopy techniques to push the understanding of membrane microdomains forward. Copyright © 2014 Elsevier Ltd. All rights reserved.

Low-temperature-compatible tunneling-current-assisted scanning microwave microscope utilizing a rigid coaxial resonator.

PubMed

Takahashi, Hideyuki; Imai, Yoshinori; Maeda, Atsutaka

2016-06-01

We present a design for a tunneling-current-assisted scanning near-field microwave microscope. For stable operation at cryogenic temperatures, making a small and rigid microwave probe is important. Our coaxial resonator probe has a length of approximately 30 mm and can fit inside the 2-in. bore of a superconducting magnet. The probe design includes an insulating joint, which separates DC and microwave signals without degrading the quality factor. By applying the SMM to the imaging of an electrically inhomogeneous superconductor, we obtain the spatial distribution of the microwave response with a spatial resolution of approximately 200 nm. Furthermore, we present an analysis of our SMM probe based on a simple lumped-element circuit model along with the near-field microwave measurements of silicon wafers having different conductivities.

Low-temperature-compatible tunneling-current-assisted scanning microwave microscope utilizing a rigid coaxial resonator

NASA Astrophysics Data System (ADS)

Takahashi, Hideyuki; Imai, Yoshinori; Maeda, Atsutaka

2016-06-01

We present a design for a tunneling-current-assisted scanning near-field microwave microscope. For stable operation at cryogenic temperatures, making a small and rigid microwave probe is important. Our coaxial resonator probe has a length of approximately 30 mm and can fit inside the 2-in. bore of a superconducting magnet. The probe design includes an insulating joint, which separates DC and microwave signals without degrading the quality factor. By applying the SMM to the imaging of an electrically inhomogeneous superconductor, we obtain the spatial distribution of the microwave response with a spatial resolution of approximately 200 nm. Furthermore, we present an analysis of our SMM probe based on a simple lumped-element circuit model along with the near-field microwave measurements of silicon wafers having different conductivities.

Electromagnetic Detection of Fatigue Cracks under Protruding Head Ferromagnetic Fasteners

NASA Technical Reports Server (NTRS)

Wincheski, Buzz; Namkung, Min

2004-01-01

The detection of fatigue cracks under installed fasteners has been a major goal of the aging aircraft NDE community. The Sliding Probe, Magneto-Optic Imager, Rotating Self-Nulling Probe, Low Frequency Eddy Current Array, and Eddyscan systems are among the instruments developed for this inspection. It has been verified that the detection of fatigue cracks under flush head aluminum and titanium fasteners can be accomplished with a high resolution by the above techniques. The detection of fatigue cracks under ferromagnetic and protruding head fasteners, however, has been found to be much more difficult. For the present work, the inspection for fatigue cracks under SAE 4340 Steel Hi-Lok fasteners is explored. Modifications to the Rotating Self-Nulling Eddy Current Probe System are presented which enable the detection of fatigue cracks hidden under the protruding head of the ferromagnetic fastener. Inspection results for samples with varying length EDM notches are shown, as well as a comparison between the signature from an EDM notch and an actual fatigue crack. Finite Element Modeling is used to investigate the effect of the ferromagnetic fastener on the induced eddy current distribution in order to help explain the detection characteristics of the system. This paper will also introduce a modification to the Rotating Probe System designed specifically for the detection of deeply buried flaws in multilayer conductors. The design change incorporates a giant magnetoresistive (GMR) sensor as the pickup device to improve the low frequency performance of the probe. The flaw detection capabilities of the GMR based Self- Nulling Probe are presented along with the status of the GMR based Rotating Probe System for detection of deeply buried flaws under installed fasteners.

Theory and application of high temperature superconducting eddy current probes for nondestructive evaluation

NASA Astrophysics Data System (ADS)

Claycomb, James Ronald

1998-10-01

Several High-T c Superconducting (HTS) eddy current probes have been developed for applications in electromagnetic nondestructive evaluation (NDE) of conducting materials. The probes utilize high-T c SUperconducting Quantum Interference Device (SQUID) magnetometers to detect the fields produced by the perturbation of induced eddy currents resulting from subsurface flaws. Localized HTS shields are incorporated to selectively screen out environmental electromagnetic interference and enable movement of the instrument in the Earth's magnetic field. High permeability magnetic shields are employed to focus flux into, and thereby increase the eddy current density in the metallic test samples. NDE test results are presented, in which machined flaws in aluminum alloy are detected by probes of different design. A novel current injection technique performing NDE of wires using SQUIDs is also discussed. The HTS and high permeability shields are designed based on analytical and numerical finite element method (FEM) calculations presented here. Superconducting and high permeability magnetic shields are modeled in uniform noise fields and in the presence of dipole fields characteristic of flaw signals. Several shield designs are characterized in terms of (1) their ability to screen out uniform background noise fields; (2) the resultant improvement in signal-to-noise ratio and (3) the extent to which dipole source fields are distorted. An analysis of eddy current induction is then presented for low frequency SQUID NDE. Analytical expressions are developed for the induced eddy currents and resulting magnetic fields produced by excitation sources above conducting plates of varying thickness. The expressions derived here are used to model the SQUID's response to material thinning. An analytical defect model is also developed, taking into account the attenuation of the defect field through the conducting material, as well as the current flow around the edges of the flaw. Time harmonic FEM calculations are then used to model the electromagnetic response of eight probe designs, consisting of an eddy current drive coil coupled to a SQUID surrounded by superconducting and/or high permeability magnetic shielding. Simulations are carried out with the eddy current probes located a finite distance above a conducting surface. Results are quantified in terms of shielding and focus factors for each probe design.

An Eddy Current Testing Platform System for Pipe Defect Inspection Based on an Optimized Eddy Current Technique Probe Design.

PubMed

Rifai, Damhuji; Abdalla, Ahmed N; Razali, Ramdan; Ali, Kharudin; Faraj, Moneer A

2017-03-13

The use of the eddy current technique (ECT) for the non-destructive testing of conducting materials has become increasingly important in the past few years. The use of the non-destructive ECT plays a key role in the ensuring the safety and integrity of the large industrial structures such as oil and gas pipelines. This paper introduce a novel ECT probe design integrated with the distributed ECT inspection system (DSECT) use for crack inspection on inner ferromagnetic pipes. The system consists of an array of giant magneto-resistive (GMR) sensors, a pneumatic system, a rotating magnetic field excitation source and a host PC acting as the data analysis center. Probe design parameters, namely probe diameter, an excitation coil and the number of GMR sensors in the array sensor is optimized using numerical optimization based on the desirability approach. The main benefits of DSECT can be seen in terms of its modularity and flexibility for the use of different types of magnetic transducers/sensors, and signals of a different nature with either digital or analog outputs, making it suited for the ECT probe design using an array of GMR magnetic sensors. A real-time application of the DSECT distributed system for ECT inspection can be exploited for the inspection of 70 mm carbon steel pipe. In order to predict the axial and circumference defect detection, a mathematical model is developed based on the technique known as response surface methodology (RSM). The inspection results of a carbon steel pipe sample with artificial defects indicate that the system design is highly efficient.

Animal Viruses Probe dataset (AVPDS) for microarray-based diagnosis and identification of viruses.

PubMed

Yadav, Brijesh S; Pokhriyal, Mayank; Vasishtha, Dinesh P; Sharma, Bhaskar

2014-03-01

AVPDS (Animal Viruses Probe dataset) is a dataset of virus-specific and conserve oligonucleotides for identification and diagnosis of viruses infecting animals. The current dataset contain 20,619 virus specific probes for 833 viruses and their subtypes and 3,988 conserved probes for 146 viral genera. Dataset of virus specific probe has been divided into two fields namely virus name and probe sequence. Similarly conserved probes for virus genera table have genus, and subgroup within genus name and probe sequence. The subgroup within genus is artificially divided subgroups with no taxonomic significance and contains probes which identifies viruses in that specific subgroup of the genus. Using this dataset we have successfully diagnosed the first case of Newcastle disease virus in sheep and reported a mixed infection of Bovine viral diarrhea and Bovine herpesvirus in cattle. These dataset also contains probes which cross reacts across species experimentally though computationally they meet specifications. These probes have been marked. We hope that this dataset will be useful in microarray-based detection of viruses. The dataset can be accessed through the link https://dl.dropboxusercontent.com/u/94060831/avpds/HOME.html.

Non-toxic fluorescent phosphonium probes to detect mitochondrial potential

NASA Astrophysics Data System (ADS)

Šarić, Ana; Crnolatac, Ivo; Bouillaud, Frédéric; Sobočanec, Sandra; Mikecin, Ana-Matea; Mačak Šafranko, Željka; Delgeorgiev, Todor; Piantanida, Ivo; Balog, Tihomir; Petit, Patrice X.

2017-03-01

We evaluated our phosphonium-based fluorescent probes for selective staining of mitochondria. Currently used probes for monitoring mitochondrial membrane potential show varying degrees of interference with cell metabolism, photo-induced damage and probe binding. Here presented probes are characterised by highly efficient cellular uptake and specific accumulation in mitochondria. Fluorescent detection of the probes was accomplished using flow cytometry and confocal microscopy imaging of yeast and mammalian cells. Toxicity analysis (impedimetry—xCELLigence for the cellular proliferation and Seahorse technology for respiratory properties) confirms that these dyes exhibit no-toxicity on mitochondrial or cellular functioning even for long time incubation. The excellent chemical and photophysical stability of the dyes makes them promising leads toward improved fluorescent probes. Therefore, the probes described here offer to circumvent the problems associated with existing-probe’s limitations.

Activity-based probes for the ubiquitin conjugation-deconjugation machinery: new chemistries, new tools, and new insights.

PubMed

Hewings, David S; Flygare, John A; Bogyo, Matthew; Wertz, Ingrid E

2017-05-01

The reversible post-translational modification of proteins by ubiquitin and ubiquitin-like proteins regulates almost all cellular processes, by affecting protein degradation, localization, and complex formation. Deubiquitinases (DUBs) are proteases that remove ubiquitin modifications or cleave ubiquitin chains. Most DUBs are cysteine proteases, which makes them well suited for study by activity-based probes. These DUB probes report on deubiquitinase activity by reacting covalently with the active site in an enzyme-catalyzed manner. They have proven to be important tools to study DUB selectivity and proteolytic activity in different settings, to identify novel DUBs, and to characterize deubiquitinase inhibitors. Inspired by the efficacy of activity-based probes for DUBs, several groups have recently reported probes for the ubiquitin conjugation machinery (E1, E2, and E3 enzymes). Many of these enzymes, while not proteases, also posses active site cysteine residues and can be targeted by covalent probes. In this review, we will discuss how features of the probe (cysteine-reactive group, recognition element, and reporter tag) affect reactivity and suitability for certain experimental applications. We will also review the diverse applications of the current probes, and discuss the need for new probe types to study emerging aspects of ubiquitin biology. © 2017 Federation of European Biochemical Societies.

Advanced electric-field scanning probe lithography on molecular resist using active cantilever

NASA Astrophysics Data System (ADS)

Kaestner, Marcus; Aydogan, Cemal; Lipowicz, Hubert-Seweryn; Ivanov, Tzvetan; Lenk, Steve; Ahmad, Ahmad; Angelov, Tihomir; Reum, Alexander; Ishchuk, Valentyn; Atanasov, Ivaylo; Krivoshapkina, Yana; Hofer, Manuel; Holz, Mathias; Rangelow, Ivo W.

2015-03-01

The routine "on demand" fabrication of features smaller than 10 nm opens up new possibilities for the realization of many novel nanoelectronic, NEMS, optical and bio-nanotechnology-based devices. Based on the thermally actuated, piezoresistive cantilever technology we have developed a first prototype of a scanning probe lithography (SPL) platform able to image, inspect, align and pattern features down to single digit nano regime. The direct, mask-less patterning of molecular resists using active scanning probes represents a promising path circumventing the problems in today's radiation-based lithography. Here, we present examples of practical applications of the previously published electric field based, current-controlled scanning probe lithography on molecular glass resist calixarene by using the developed tabletop SPL system. We demonstrate the application of a step-and-repeat scanning probe lithography scheme including optical as well as AFM based alignment and navigation. In addition, sequential read-write cycle patterning combining positive and negative tone lithography is shown. We are presenting patterning over larger areas (80 x 80 μm) and feature the practical applicability of the lithographic processes.

Evaluation of ion collection area in Faraday probes.

PubMed

Brown, Daniel L; Gallimore, Alec D

2010-06-01

A Faraday probe with three concentric rings was designed and fabricated to assess the effect of gap width and collector diameter in a systematic study of the diagnostic ion collection area. The nested Faraday probe consisted of two concentric collector rings and an outer guard ring, which enabled simultaneous current density measurements on the inner and outer collectors. Two versions of the outer collector were fabricated to create gaps of 0.5 and 1.5 mm between the rings. Distribution of current density in the plume of a low-power Hall thruster ion source was measured in azimuthal sweeps at constant radius from 8 to 20 thruster diameters downstream of the exit plane with variation in facility background pressure. A new analytical technique is proposed to account for ions collected in the gap between the Faraday probe collector and guard ring. This method is shown to exhibit excellent agreement between all nested Faraday probe configurations, and to reduce the magnitude of integrated ion beam current to levels consistent with Hall thruster performance analyses. The technique is further studied by varying the guard ring bias potential with a fixed collector bias potential, thereby controlling ion collection in the gap. Results are in agreement with predictions based on the proposed analytical technique. The method is applied to a past study comparing the measured ion current density profiles of two Faraday probe designs. These findings provide new insight into the nature of ion collection in Faraday probe diagnostics, and lead to improved accuracy with a significant reduction in measurement uncertainty.

Energy-Based Tetrahedron Sensor for High-Temperature, High-Pressure Environments

NASA Technical Reports Server (NTRS)

Gee, Kent L.; Sommerfeldt, Scott D.; Blotter, Jonathan D.

2012-01-01

An acoustic energy-based probe has been developed that incorporates multiple acoustic sensing elements in order to obtain the acoustic pressure and three-dimensional acoustic particle velocity. With these quantities, the user can obtain various energy-based quantities, including acoustic energy density, acoustic intensity, and acoustic impedance. In this specific development, the probe has been designed to operate in an environment characterized by high temperatures and high pressures as is found in the close vicinity of rocket plumes. Given these capabilities, the probe is designed to be used to investigate the acoustic conditions within the plume of a rocket engine or jet engine to facilitate greater understanding of the noise generation mechanisms in those plumes. The probe features sensors mounted inside a solid sphere. The associated electronics for the probe are contained within the sphere and the associated handle for the probe. More importantly, the design of the probe has desirable properties that reduce the bias errors associated with determining the acoustic pressure and velocity using finite sum and difference techniques. The diameter of the probe dictates the lower and upper operating frequencies for the probe, where accurate measurements can be acquired. The current probe design implements a sphere diameter of 1 in. (2.5 cm), which limits the upper operating frequency to about 4.5 kHz. The sensors are operational up to much higher frequencies, and could be used to acquire pressure data at higher frequencies, but the energy-based measurements are limited to that upper frequency. Larger or smaller spherical probes could be designed to go to lower or higher frequency range

Probe compensation in cylindrical near-field scanning: A novel simulation methodology

NASA Technical Reports Server (NTRS)

Hussein, Ziad A.; Rahmat-Samii, Yahya

1993-01-01

Probe pattern compensation is essential in near-field scanning geometry, where there is a great need to accurately know far-field patterns at wide angular range. This paper focuses on a novel formulation and computer simulation to determine the precise need for and effect of probe compensation in cylindrical near-field scanning. The methodology is applied to a linear test array antenna and the NASA scatterometer radar antenna. The formulation is based on representing the probe by its equivalent tangential magnetic currents. The interaction between the probe equivalent aperture currents and the test antenna fields is obtained with the application of a reciprocity theorem. This allows us to obtain the probe vector output pickup integral which is proportional to the amplitude and phase of the electric field induced in the probe aperture with respect to its position to the test antenna. The integral is evaluated for each probe position on the required sampling point on a cylindrical near-field surface enclosing the antenna. The use of a hypothetical circular-aperture probe with a different radius permits us to derive closed-form expressions for its far-field radiation patterns. These results, together with the probe vector output pickup, allow us to perform computer simulated synthetic measurements. The far-field patterns of the test antenna are formulated based on cylindrical wave expansions of both the probe and test antenna fields. In the limit as the probe radius becomes very small, the probe vector output is the direct response of the near-field at a point, and no probe compensation is needed. Useful results are generated to compare the far-field pattern of the test antenna constructed from the knowledge of the simulated near-field with and without probe pattern compensation and the exact results. These results are important since they clearly illustrate the angular range over which probe compensation is needed. It has been found that a probe with an aperture radius of 0.25(lambda), 0.5(lambda), and 1(lambda) needs a little probe compensation, if any, near the test antenna main beam. In addition, a probe with low directivity may provide a better signal-to-noise ratio than a highly directive one. This is evident in test antenna patterns without probe compensation at wide angles.

Compensation of the sheath effects in cylindrical floating probes

NASA Astrophysics Data System (ADS)

Park, Ji-Hwan; Chung, Chin-Wook

2018-05-01

In cylindrical floating probe measurements, the plasma density and electron temperature are overestimated due to sheath expansion and oscillation. To reduce these sheath effects, a compensation method based on well-developed floating sheath theories is proposed and applied to the floating harmonic method. The iterative calculation of the Allen-Boyd-Reynolds equation can derive the floating sheath thickness, which can be used to calculate the effective ion collection area; in this way, an accurate ion density is obtained. The Child-Langmuir law is used to calculate the ion harmonic currents caused by sheath oscillation of the alternating-voltage-biased probe tip. Accurate plasma parameters can be obtained by subtracting these ion harmonic currents from the total measured harmonic currents. Herein, the measurement principles and compensation method are discussed in detail and an experimental demonstration is presented.

An Eddy Current Testing Platform System for Pipe Defect Inspection Based on an Optimized Eddy Current Technique Probe Design

PubMed Central

Rifai, Damhuji; Abdalla, Ahmed N.; Razali, Ramdan; Ali, Kharudin; Faraj, Moneer A.

2017-01-01

The use of the eddy current technique (ECT) for the non-destructive testing of conducting materials has become increasingly important in the past few years. The use of the non-destructive ECT plays a key role in the ensuring the safety and integrity of the large industrial structures such as oil and gas pipelines. This paper introduce a novel ECT probe design integrated with the distributed ECT inspection system (DSECT) use for crack inspection on inner ferromagnetic pipes. The system consists of an array of giant magneto-resistive (GMR) sensors, a pneumatic system, a rotating magnetic field excitation source and a host PC acting as the data analysis center. Probe design parameters, namely probe diameter, an excitation coil and the number of GMR sensors in the array sensor is optimized using numerical optimization based on the desirability approach. The main benefits of DSECT can be seen in terms of its modularity and flexibility for the use of different types of magnetic transducers/sensors, and signals of a different nature with either digital or analog outputs, making it suited for the ECT probe design using an array of GMR magnetic sensors. A real-time application of the DSECT distributed system for ECT inspection can be exploited for the inspection of 70 mm carbon steel pipe. In order to predict the axial and circumference defect detection, a mathematical model is developed based on the technique known as response surface methodology (RSM). The inspection results of a carbon steel pipe sample with artificial defects indicate that the system design is highly efficient. PMID:28335399

Dissociable neural mechanisms underlying response-based and familiarity-based conflict in working memory.

PubMed

Nelson, James K; Reuter-Lorenz, Patricia A; Sylvester, Ching-Yune C; Jonides, John; Smith, Edward E

2003-09-16

Cognitive control requires the resolution of interference among competing and potentially conflicting representations. Such conflict can emerge at different points between stimulus input and response generation, with the net effect being that of compromising performance. The goal of this article was to dissociate the neural mechanisms underlying different sources of conflict to elucidate the architecture of the neural systems that implement cognitive control. By using functional magnetic resonance imaging and a verbal working memory task (item recognition), we examined brain activity related to two kinds of conflict with comparable behavioral consequences. In a trial of our item-recognition task, participants saw four letters, followed by a retention interval, and a probe letter that did or did not match one of the letters held in working memory (positive probe and negative probe, respectively). On some trials, conflict arose solely because of the current negative probe having a high familiarity, due to its membership in the immediately preceding trial's target set. On other trials, additional conflict arose because of the current negative probe having also been a positive probe on the immediately preceding trial, producing response-level conflict. Consistent with previous work, conflict due to high familiarity was associated with left prefrontal activation, but not with anterior cingulate activation. The response-conflict condition, when compared with high-familiarity conflict trials, was associated with anterior cingulate cortex activation, but with no additional left prefrontal activation. This double dissociation points to differing contributions of specific cortical areas to cognitive control, which are based on the source of conflict.

Investigation of Coatings for Langmuir Probes in an Oxygen-Rich Space Environment

NASA Astrophysics Data System (ADS)

Samaniego, J. I.; Wang, X.; Andersson, L.; Malaspina, D.; Ergun, R.; Horanyi, M.

2017-12-01

The surface properties of the Langmuir probes, such as the one on the MAVEN mission, will change after exposure to upper planetary atmospheres where high concentrations of atomic oxygen and other oxidizing compounds are present. TiN (Titanium Nitride) or DAG (a resin based graphite dispersion) are the most common coatings for current Langmuir probes, yet both of these coatings pose issues when exposed to oxygen-rich space environment. TiN showed reduced surface conductivity while the DAG layers erode with exposure to oxygen. It is known that Iridium (Ir) and Rhenium (Rh) are difficult to oxidize and maintain high conductivity even in their oxidized forms, suggesting them to be good candidates for probe coatings. Oxidation of most metals creates a resistive layer on the surface of the probe that will affect the amount of current being collected at a given voltage during the probe sweep and therefore affect the accuracy of plasma parameters determined by the Langmuir probe (e.g. density, temperature). We present the results of the oxidation effect on the current-voltage curves (I-V curves) and therefore the resulting measured plasma parameters of Ir and Rh wire probes compared with other control metals and coatings (Cu, Ni, TiN) in controlled plasma environments. The oxidation process is performed in an oxygen plasma chamber in which both O+ and O2+ are created and accelerated toward the probes with energies < 10 eV. An argon plasma chamber is used to compare the probe's I-V curves before and after the oxidation process. Our preliminary results indicate that iridium shows the least effect of oxidation on the probe measurements. The second objective of this study is to identify methods that can be used in orbit to clean the surface of Langmuir probes to minimize the effect of exposure to oxidizing compounds.

Implications of contamination and surface area ratios for Langmuir probe diagnostics on CubeSats

NASA Astrophysics Data System (ADS)

Suresh, P.; Swenson, C.

2009-12-01

Theories describing the current collected by a biased probe under various conditions are necessary for such observation to be used to accurately determine plasma properties. Langmuir probes are routinely used on spacecraft to measure plasma parameters such as density, temperature, and vehicle charging. The collected current is a function of the potential between the surrounding plasma and probe surface. There have been both observations of and concepts for unaccounted variations of this potential which limit the application of Langmuir probe theory for determining plasma properties. These variations occur due to spatial variations of the work function across the probe surface due to non-uniformity of the crystalline surface properties and surface contamination of the probe. Currently we do not have theoretical expressions which consider these factors as first principles in their derivation. In the event of these surface potential variations, the analysis of the plasma using the currently available theories of the Langmuir probe yield erroneous results. We present a theory which models the current as a function of the surface potential variations. Another consideration for Langmuir probes on CubeSats is the ratio of the probe area to the return current collection area. If the area ratio is unfavorable this can also lead to erroneous results in the interpretation of observations. A mathematical formulation of the current collected by the probe for contaminated surfaces is presented and compared with data from a Langmuir probe flown on a sounding rocket mission. The implications of using Langmuir probes on CubeSats given the engineering limitations of probe cleanliness and area ratios are reviewed.

Ring Current Pressure Estimation withRAM-SCB using Data Assimilation and VanAllen Probe Flux Data

NASA Astrophysics Data System (ADS)

Godinez, H. C.; Yu, Y.; Henderson, M. G.; Larsen, B.; Jordanova, V.

2015-12-01

Capturing and subsequently modeling the influence of tail plasma injections on the inner magnetosphere is particularly important for understanding the formation and evolution of Earth's ring current. In this study, the ring current distribution is estimated with the Ring Current-Atmosphere Interactions Model with Self-Consistent Magnetic field (RAM-SCB) using, for the first time, data assimilation techniques and particle flux data from the Van Allen Probes. The state of the ring current within the RAM-SCB is corrected via an ensemble based data assimilation technique by using proton flux from one of the Van Allen Probes, to capture the enhancement of ring current following an isolated substorm event on July 18 2013. The results show significant improvement in the estimation of the ring current particle distributions in the RAM-SCB model, leading to better agreement with observations. This newly implemented data assimilation technique in the global modeling of the ring current thus provides a promising tool to better characterize the effect of substorm injections in the near-Earth regions. The work is part of the Space Hazards Induced near Earth by Large, Dynamic Storms (SHIELDS) project in Los Alamos National Laboratory.

Diffraction modeling of finite subband EFC probing on dark hole contrast with WFIRST-CGI shaped pupil coronagraph

NASA Astrophysics Data System (ADS)

Zhou, Hanying; Krist, John; Nemati, Bijan

2016-08-01

Current coronagraph instrument design (CGI), as a part of a proposed NASA WFIRST (Wide-Field InfraRed Survey Telescope) mission, allocates two subband filters per full science band in order to contain system complexity and cost. We present our detailed investigation results on the adequacy of such limited number of finite subband filters in achieving full band dark hole contrast with shaped pupil coronagraph. The study is based on diffraction propagation modeling with realistic WFIRST optics, where each subband's complex field estimation is obtained, using Electric Field Conjugation (EFC) wavefront sensing / control algorithm, from pairwise pupil plane deformable mirror (DM) probing and image plane intensity averaging of the resulting fields of multiple (subband) wavelengths. Multiple subband choices and probing and control strategies are explored, including standard subband probing; mixed wavelength and/or weighted Jacobian matrix; subband probing with intensity subtraction; and extended subband probing with intensity subtraction. Overall, the investigation shows that the achievable contrast with limited number of finite subband EFC probing is about 2 2.5x worse than the designed post-EFC contrast for current SPC design. The result suggests that for future shaped pupil design, slightly larger over intended full bandwidth should be considered if it will be used with limited subbands for probing.

Ring current electron dynamics during geomagnetic storms based on the Van Allen Probes measurements: Ring Current Electrons

DOE PAGES

Zhao, H.; Li, X.; Baker, D. N.; ...

2016-04-16

Based on comprehensive measurements from Helium, Oxygen, Proton, and Electron Mass Spectrometer Ion Spectrometer, Relativistic Electron-Proton Telescope, and Radiation Belt Storm Probes Ion Composition Experiment instruments on the Van Allen Probes, comparative studies of ring current electrons and ions are performed and the role of energetic electrons in the ring current dynamics is investigated. The deep injections of tens to hundreds of keV electrons and tens of keV protons into the inner magnetosphere occur frequently; after the injections the electrons decay slowly in the inner belt but protons in the low L region decay very fast. Intriguing similarities between lowermore » energy protons and higher-energy electrons are also found. The evolution of ring current electron and ion energy densities and energy content are examined in detail during two geomagnetic storms, one moderate and one intense. Here, the results show that the contribution of ring current electrons to the ring current energy content is much smaller than that of ring current ions (up to ~12% for the moderate storm and ~7% for the intense storm), and <35 keV electrons dominate the ring current electron energy content at the storm main phases. Though the electron energy content is usually much smaller than that of ions, the enhancement of ring current electron energy content during the moderate storm can get to ~30% of that of ring current ions, indicating a more dynamic feature of ring current electrons and important role of electrons in the ring current buildup. Lastly, the ring current electron energy density is also shown to be higher at midnight and dawn while lower at noon and dusk.« less

Study of entry and landing probes for exploration of Titan

NASA Technical Reports Server (NTRS)

1979-01-01

Saturn's largest moon, Titan, is a totally unique planetary body which is certain to yield exciting new phenomena. Current information is lacking in detail to distinguish between a thin methane rich atmosphere and a thick nitrogen rich atmosphere. Therefore, both the thin and thick atmospheric models were used for the study of various Titan probe classes described in this report. The technical requirements, conceptual design, science return, schedule, cost and mission implications of three probe classes that could be used for exploration of Titan are defined. The three probe classes were based on a wide range of exploration mission possibilities.

A novel constant-force scanning probe incorporating mechanical-magnetic coupled structures.

PubMed

Wang, Hongxi; Zhao, Jian; Gao, Renjing; Yang, Yintang

2011-07-01

A one-dimensional scanning probe with constant measuring force is designed and fabricated by utilizing the negative stiffness of the magnetic coupled structure, which mainly consists of the magnetic structure, the parallel guidance mechanism, and the pre-stressed spring. Based on the theory of material mechanics and the equivalent surface current model for computing the magnetic force, the analytical model of the scanning probe subjected to multi-forces is established, and the nonlinear relationship between the measuring force and the probe displacement is obtained. The practicability of introducing magnetic coupled structure in the constant-force probe is validated by the consistency of the results in numerical simulation and experiments.

Detection of an electron beam in a high density plasma via an electrostatic probe

NASA Astrophysics Data System (ADS)

Majeski, Stephen; Yoo, Jongsoo; Zweben, Stewart; Yamada, Masaaki

2018-07-01

An electron beam is detected by a 1D floating potential probe array in a relatively high density (1012–1013 cm‑3) and low temperature (∼5 eV) plasma of the Magnetic Reconnection Experiment. Clear perturbations in the floating potential profile by the electron beam are observed. Based on the floating potential profile and a current balance equation to the probe array tips, the effective width of the electron beam is determined, from which we determine the radial and toroidal beam current density profiles. After the profile of the electron beam is specified from the measured beam current, we demonstrate the consistency of the current balance equation and the location of the perturbation is also in agreement with field line mapping. No significant broadening of the electron beam is observed after the beam propagates for tens of centimeters through the high density plasma. These results prove that the field line mapping is, in principle, possible in high density plasmas.

Detection of an electron beam in a high density plasma via an electrostatic probe

DOE Office of Scientific and Technical Information (OSTI.GOV)

Majeski, Stephen; Yoo, Jongsoo; Zweben, Stewart

Here, an electron beam is detected by a 1D floating potential probe array in a relatively high density (10 12–10 13 cm -3) and low temperature (~5 eV) plasma of the Magnetic Reconnection Experiment. Clear perturbations in the floating potential profile by the electron beam are observed. Based on the floating potential profile and a current balance equation to the probe array tips, the effective width of the electron beam is determined, from which we determine the radial and toroidal beam current density profiles. After the profile of the electron beam is specified from the measured beam current, we demonstratemore » the consistency of the current balance equation and the location of the perturbation is also in agreement with field line mapping. No significant broadening of the electron beam is observed after the beam propagates for tens of centimeters through the high density plasma. These results prove that the field line mapping is, in principle, possible in high density plasmas.« less

Detection of an electron beam in a high density plasma via an electrostatic probe

DOE PAGES

Majeski, Stephen; Yoo, Jongsoo; Zweben, Stewart; ...

2018-05-08

Here, an electron beam is detected by a 1D floating potential probe array in a relatively high density (10 12–10 13 cm -3) and low temperature (~5 eV) plasma of the Magnetic Reconnection Experiment. Clear perturbations in the floating potential profile by the electron beam are observed. Based on the floating potential profile and a current balance equation to the probe array tips, the effective width of the electron beam is determined, from which we determine the radial and toroidal beam current density profiles. After the profile of the electron beam is specified from the measured beam current, we demonstratemore » the consistency of the current balance equation and the location of the perturbation is also in agreement with field line mapping. No significant broadening of the electron beam is observed after the beam propagates for tens of centimeters through the high density plasma. These results prove that the field line mapping is, in principle, possible in high density plasmas.« less

Langmuir-Probe Measurements in Flowing-Afterglow Plasmas

NASA Technical Reports Server (NTRS)

Johnsen, R.; Shunko, E. V.; Gougousi, T.; Golde, M. F.

1994-01-01

The validity of the orbital-motion theory for cylindrical Langmuir probes immersed in flowing- afterglow plasmas is investigated experimentally. It is found that the probe currents scale linearly with probe area only for electron-collecting but not for ion-collecting probes. In general, no agreement is found between the ion and electron densities derived from the probe currents. Measurements in recombining plasmas support the conclusion that only the electron densities derived from probe measurements can be trusted to be of acceptable accuracy. This paper also includes a brief derivation of the orbital-motion theory, a discussion of perturbations of the plasma by the probe current, and the interpretation of plasma velocities obtained from probe measurements.

Development and applications of single particle orientation and rotational tracking in dynamic systems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chen, Kuangcai

The goal of this study is to help with future data analysis and experiment designs in rotational dynamics research using DIC-based SPORT technique. Most of the current studies using DIC-based SPORT techniques are technical demonstrations. Understanding the mechanisms behind the observed rotational behaviors of the imaging probes should be the focus of the future SPORT studies. More efforts are still needed in the development of new imaging probes, particle tracking methods, instrumentations, and advanced data analysis methods to further extend the potential of DIC-based SPORT technique.

Design strategy for photoinduced electron transfer-based small-molecule fluorescent probes of biomacromolecules.

PubMed

Zhang, Wei; Ma, Zhao; Du, Lupei; Li, Minyong

2014-06-07

As the cardinal support of innumerable biological processes, biomacromolecules such as proteins, nucleic acids and polysaccharides are of importance to living systems. The key to understanding biological processes is to realize the role of these biomacromolecules in thte localization, distribution, conformation and interaction with other molecules. With the current development and adaptation of fluorescent technologies in biomedical and pharmaceutical fields, the fluorescence imaging (FLI) approach of using small-molecule fluorescent probes is becoming an up-to-the-minute method for the detection and monitoring of these imperative biomolecules in life sciences. However, conventional small-molecule fluorescent probes may provide undesirable results because of their intrinsic deficiencies such as low signal-to-noise ratio (SNR) and false-positive errors. Recently, small-molecule fluorescent probes with a photoinduced electron transfer (PET) "on/off" switch for biomacromolecules have been thoroughly considered. When recognized by the biomacromolecules, these probes turn on/off the PET switch and change the fluorescence intensity to present a high SNR result. It should be emphasized that these PET-based fluorescent probes could be advantageous for understanding the pathogenesis of various diseases caused by abnormal expression of biomacromolecules. The discussion of this successful strategy involved in this review will be a valuable guide for the further development of new PET-based small-molecule fluorescent probes for biomacromolecules.

Electrochemical current rectification-a novel signal amplification strategy for highly sensitive and selective aptamer-based biosensor.

PubMed

Feng, Lingyan; Sivanesan, Arumugam; Lyu, Zhaozi; Offenhäusser, Andreas; Mayer, Dirk

2015-04-15

Electrochemical aptamer-based (E-AB) sensors represent an emerging class of recently developed sensors. However, numerous of these sensors are limited by a low surface density of electrode-bound redox-oligonucleotides which are used as probe. Here we propose to use the concept of electrochemical current rectification (ECR) for the enhancement of the redox signal of E-AB sensors. Commonly, the probe-DNA performs a change in conformation during target binding and enables a nonrecurring charge transfer between redox-tag and electrode. In our system, the redox-tag of the probe-DNA is continuously replenished by solution-phase redox molecules. A unidirectional electron transfer from electrode via surface-linked redox-tag to the solution-phase redox molecules arises that efficiently amplifies the current response. Using this robust and straight-forward strategy, the developed sensor showed a substantial signal amplification and consequently improved sensitivity with a calculated detection limit of 114nM for ATP, which was improved by one order of magnitude compared with the amplification-free detection and superior to other previous detection results using enzymes or nanomaterials-based signal amplification. To the best of our knowledge, this is the first demonstration of an aptamer-based electrochemical biosensor involving electrochemical rectification, which can be presumably transferred to other biomedical sensor systems. Copyright © 2014 Elsevier B.V. All rights reserved.

Rational Design of in Vivo Tau Tangle-Selective Near-Infrared Fluorophores: Expanding the BODIPY Universe.

PubMed

Verwilst, Peter; Kim, Hye-Ri; Seo, Jinho; Sohn, Nak-Won; Cha, Seung-Yun; Kim, Yeongmin; Maeng, Sungho; Shin, Jung-Won; Kwak, Jong Hwan; Kang, Chulhun; Kim, Jong Seung

2017-09-27

The elucidation of the cause of Alzheimer's disease remains one of the greatest questions in neurodegenerative research. The lack of highly reliable low-cost sensors to study the structural changes in key proteins during the progression of the disease is a contributing factor to this lack of insight. In the current work, we describe the rational design and synthesis of two fluorescent BODIPY-based probes, named Tau 1 and Tau 2. The probes were evaluated on the molecular surface formed by a fibril of the PHF6 ( 306 VQIVYK 311 ) tau fragment using molecular docking studies to provide a potential molecular model to rationalize the selectivity of the new probes as compared to a homologous Aβ-selective probe. The probes were synthesized in a few steps from commercially available starting products and could thus prove to be highly cost-effective. We demonstrated the excellent photophysical properties of the dyes, such as a large Stokes shift and emission in the near-infrared window of the electromagnetic spectrum. The probes demonstrated a high selectivity for self-assembled microtubule-associated protein tau (Tau protein), in both solution and cell-based experiments. Moreover, the administration to an acute murine model of tauopathy clearly revealed the staining of self-assembled hyperphosphorylated tau protein in pathologically relevant hippocampal brain regions. Tau 1 demonstrated efficient blood-brain barrier penetrability and demonstrated a clear selectivity for tau tangles over Aβ plaques, as well as the capacity for in vivo imaging in a transgenic mouse model. The current work could open up avenues for the cost-effective monitoring of the tau protein aggregation state in animal models as well as tissue staining. Furthermore, these fluorophores could serve as the basis for the development of clinically relevant sensors, for example based on PET imaging.

Revolving Eddy-Current Probe Detects Cracks Near Rivets

NASA Technical Reports Server (NTRS)

Namkung, Min; Wincheski, Buzz; Fulton, James P.; Nath, Shridhar; Simpson, John

1995-01-01

Scanning eddy-current probe in circular pattern increases sensitivity with which probe indicates fatigue cracks and other defects in metal surfaces in vicinity of rivets. Technique devised to facilitate inspection of riveted joints in aircraft. Eddy-current probe in question described in "Electro-magnetic Flaw Detector Is Easier To Use" (LAR-15046).

Distance Probes of Dark Energy

DOE PAGES

Kim, A. G.; Padmanabhan, N.; Aldering, G.; ...

2015-03-15

We present the results from the Distances subgroup of the Cosmic Frontier Community Planning Study (Snowmass 2013). This document summarizes the current state of the field as well as future prospects and challenges. In addition to the established probes using Type Ia supernovae and baryon acoustic oscillations, we also consider prospective methods based on clusters, active galactic nuclei, gravitational wave sirens and strong lensing time delays.

Magnetic Diagnosis Upgrade and Analysis for MHD Instabilities on the J-TEXT

NASA Astrophysics Data System (ADS)

Guo, Daojing; Hu, Qiming; Zhuang, Ge; Wang, Nengchao; Ding, Yonghua; Tang, Yuejin; Yu, Qingquan; Huazhong University of Science; Technology Team; Max-Planck-Institut für Plasmaphysik Collaboration

2017-10-01

The magnetic diagnostic system on the J-TEXT tokamak has been upgraded to measure the magnetohydrodynamic (MHD) instabilities with diverse bands of frequencies. 12 saddle loop probes and 73 Mirnov probes are newly developed. The fabrication and installment of the new probes are elaborately designed, in consideration of higher spatial resolution and better amplitude-frequency characteristic. In this case, the probes utilize two kinds of novel fabrication craft, one of which is low temperature co-fired ceramics (LTCC), the other is flexible printed circuit (FPC). A great deal of experiments on the J-TEXT have validated the stability of the new system. Some typical discharges observed by the new diagnostic system are reviewed. In order to extract useful information from raw signals, several efficient signal processing methods are reviewed. An analytical model based on lumped eddy current circuits is used to compensate equilibrium flux and the corresponding eddy current fluxes, a visualization processing based on singular value decomposition (SVD) and cross-power spectrum are applied to detect the mode number. Fusion Science Program of China (Contract Nos. 2015GB111001 and 2014GB108000) and the National Natural Science Foundation of China (Contract Nos. 11505069 and 11405068).

Probing possible decoherence effects in atmospheric neutrino oscillations.

PubMed

Lisi, E; Marrone, A; Montanino, D

2000-08-07

It is shown that the results of the Super-Kamiokande atmospheric neutrino experiment, interpreted in terms of nu(mu)<-->nu(tau) flavor transitions, can probe possible decoherence effects induced by new physics (e.g., by quantum gravity) with high sensitivity, supplementing current laboratory tests based on kaon oscillations and on neutron interferometry. By varying the (unknown) energy dependence of such effects, one can either obtain strong limits on their amplitude or use them to find an unconventional solution to the atmospheric nu anomaly based solely on decoherence.

Development of Tuning Fork Based Probes for Atomic Force Microscopy

NASA Astrophysics Data System (ADS)

Jalilian, Romaneh; Yazdanpanah, Mehdi M.; Torrez, Neil; Alizadeh, Amirali; Askari, Davood

2014-03-01

This article reports on the development of tuning fork-based AFM/STM probes in NaugaNeedles LLC for use in atomic force microscopy. These probes can be mounted on different carriers per customers' request. (e.g., RHK carrier, Omicron carrier, and tuning fork on a Sapphire disk). We are able to design and engineer tuning forks on any type of carrier used in the market. We can attach three types of tips on the edge of a tuning fork prong (i.e., growing Ag2Ga nanoneedles at any arbitrary angle, cantilever of AFM tip, and tungsten wire) with lengths from 100-500 μm. The nanoneedle is located vertical to the fork. Using a suitable insulation and metallic coating, we can make QPlus sensors that can detect tunneling current during the AFM scan. To make Qplus sensors, the entire quartz fork will be coated with an insulating material, before attaching the nanoneedle. Then, the top edge of one prong is coated with a thin layer of conductive metal and the nanoneedle is attached to the fork end of the metal coated prong. The metal coating provides electrical connection to the tip for tunneling current readout and to the electrodes and used to read the QPlus current. Since the amount of mass added to the fork is minimal, the resonance frequency spectrum does not change and still remains around 32.6 KHz and the Q factor is around 1,200 in ambient condition. These probes can enhance the performance of tuning fork based atomic microscopy.

Determination of plasma displacement based on eddy current diagnostics for the Keda Torus eXperiment

NASA Astrophysics Data System (ADS)

Tu, Cui; Li, Hong; Liu, Adi; Li, Zichao; Zhang, Yuan; You, Wei; Tan, Mingsheng; Luo, Bing; Adil, Yolbarsop; Hu, Jintong; Wu, Yanqi; Yan, Wentan; Xie, Jinlin; Lan, Tao; Mao, Wenzhe; Ding, Weixing; Xiao, Chijin; Zhuang, Ge; Liu, Wandong

2017-10-01

The measurement of plasma displacement is one of the most basic diagnostic tools in the study of plasma equilibrium and control in a toroidal magnetic confinement configuration. During pulse discharge, the eddy current induced in the vacuum vessel and shell will produce an additional magnetic field at the plasma boundary, which will have a significant impact on the measurement of plasma displacement using magnetic probes. In the newly built Keda Torus eXperiment (KTX) reversed field pinch device, the eddy current in the composite shell can be obtained at a high spatial resolution. This device offers a new way to determine the plasma displacement for KTX through the multipole moment expansion of the eddy current, which can be obtained by unique probe arrays installed on the inner and outer surfaces of the composite shell. In an ideal conductor shell approximation, the method of multipole moment expansion of the poloidal eddy current for measuring the plasma displacement in toroidal coordinates, is more accurate than the previous method based on symmetrical magnetic probes, which yielded results in cylindrical coordinates. Through an analytical analysis of many current filaments and numerical simulations of the current distribution in toroidal coordinates, the scaling relation between the first moment of the eddy current and the center of gravity of the plasma current is obtained. In addition, the origin of the multipole moment expansion of the eddy current in KTX is retrieved simultaneously. Preliminary data on the plasma displacement have been collected using these two methods during short pulse discharges in the KTX device, and the results of the two methods are in reasonable agreement.

On the use of the double floating probe method to infer the difference between the electron and the heavy particles temperatures in an atmospheric pressure, vortex-stabilized nitrogen plasma jet

NASA Astrophysics Data System (ADS)

Prevosto, L.; Kelly, H.; Mancinelli, B. R.

2014-05-01

Sweeping double probe measurements in an atmospheric pressure direct current vortex-stabilized plasma jet are reported (plasma conditions: 100 A discharge current, N2 gas flow rate of 25 Nl/min, thoriated tungsten rod-type cathode, copper anode with 5 mm inner diameter). The interpretation of the double probe characteristic was based on a generalization of the standard double floating probe formulae for non-uniform plasmas coupled to a non-equilibrium plasma composition model. Perturbations caused by the current to the probe together with collisional and thermal processes inside the probe perturbed region were taken into account. Radial values of the average electron and heavy particle temperatures as well as the electron density were obtained. The calculation of the temperature values did not require any specific assumption about a temperature relationship between different particle species. An electron temperature of 10 900 ± 900 K, a heavy particle temperature of 9300 ± 900 K, and an electron density of about 3.5 × 1022 m-3 were found at the jet centre at 3.5 mm downstream from the torch exit. Large deviations from kinetic equilibrium were found toward the outer border of the plasma jet. These results showed good agreement with those previously reported by the authors by using a single probe technique. The calculations have shown that this method is particularly useful for studying spraying-type plasma torches operated at power levels of about 15 kW.

On the use of the double floating probe method to infer the difference between the electron and the heavy particles temperatures in an atmospheric pressure, vortex-stabilized nitrogen plasma jet.

PubMed

Prevosto, L; Kelly, H; Mancinelli, B R

2014-05-01

Sweeping double probe measurements in an atmospheric pressure direct current vortex-stabilized plasma jet are reported (plasma conditions: 100 A discharge current, N2 gas flow rate of 25 Nl/min, thoriated tungsten rod-type cathode, copper anode with 5 mm inner diameter). The interpretation of the double probe characteristic was based on a generalization of the standard double floating probe formulae for non-uniform plasmas coupled to a non-equilibrium plasma composition model. Perturbations caused by the current to the probe together with collisional and thermal processes inside the probe perturbed region were taken into account. Radial values of the average electron and heavy particle temperatures as well as the electron density were obtained. The calculation of the temperature values did not require any specific assumption about a temperature relationship between different particle species. An electron temperature of 10,900 ± 900 K, a heavy particle temperature of 9300 ± 900 K, and an electron density of about 3.5 × 10(22) m(-3) were found at the jet centre at 3.5 mm downstream from the torch exit. Large deviations from kinetic equilibrium were found toward the outer border of the plasma jet. These results showed good agreement with those previously reported by the authors by using a single probe technique. The calculations have shown that this method is particularly useful for studying spraying-type plasma torches operated at power levels of about 15 kW.

[Bioimpedance means of skin condition monitoring during therapeutic and cosmetic procedures].

PubMed

Alekseenko, V A; Kus'min, A A; Filist, S A

2008-01-01

Engineering and technological problems of bioimpedance skin surface mapping are considered. A typical design of a device based on a PIC 16F microcontroller is suggested. It includes a keyboard, LCD indicator, probing current generator with programmed frequency tuning, and units for probing current monitoring and bioimpedance measurement. The electrode matrix of the device is constructed using nanotechnology. A microcontroller-controlled multiplexor provides scanning of interelectrode impedance, which makes it possible to obtain the impedance image of the skin surface under the electrode matrix. The microcontroller controls the probing signal generator frequency and allows layer-by-layer images of skin under the electrode matrix to be obtained. This makes it possible to use reconstruction tomography methods for analysis and monitoring of the skin condition during therapeutic and cosmetic procedures.

Solving the Capacitive Effect in the High-Frequency sweep for Langmuir Probe in SYMPLE

NASA Astrophysics Data System (ADS)

Pramila; Patel, J. J.; Rajpal, R.; Hansalia, C. J.; Anitha, V. P.; Sathyanarayana, K.

2017-04-01

Langmuir Probe based measurements need to be routinely carried out to measure various plasma parameters such as the electron density (ne), the electron temperature (Te), the floating potential (Vf), and the plasma potential (Vp). For this, the diagnostic electronics along with the biasing power supplies is installed in standard industrial racks with a 2KV isolation transformer. The Signal Conditioning Electronics (SCE) system is populated inside the 4U-chassis based system with the front-end electronics, designed using high common mode differential amplifiers which can measure small differential signal in presence of high common mode dc- bias or ac ramp voltage used for biasing the probes. DC-biasing of the probe is most common method for getting its I-V characteristic but method of biasing the probe with a sweep at high frequency encounters the problem of corruption of signal due to capacitive effect specially when the sweep period and the discharge time is very fast and die down in the order of μs or lesser. This paper presents and summarises the method of removing such effects encountered while measuring the probe current.

Individually addressable vertically aligned carbon nanofiber-based electrochemical probes

NASA Astrophysics Data System (ADS)

Guillorn, M. A.; McKnight, T. E.; Melechko, A.; Merkulov, V. I.; Britt, P. F.; Austin, D. W.; Lowndes, D. H.; Simpson, M. L.

2002-03-01

In this paper we present the fabrication and initial testing results of high aspect ratio vertically aligned carbon nanofiber (VACNF)-based electrochemical probes. Electron beam lithography was used to define the catalytic growth sites of the VACNFs. Following catalyst deposition, VACNF were grown using a plasma enhanced chemical vapor deposition process. Photolithography was performed to realize interconnect structures. These probes were passivated with a thin layer of SiO2, which was then removed from the tips of the VACNF, rendering them electrochemically active. We have investigated the functionality of completed devices using cyclic voltammetry (CV) of ruthenium hexammine trichloride, a highly reversible, outer sphere redox system. The faradaic current obtained during CV potential sweeps shows clear oxidation and reduction peaks at magnitudes that correspond well with the geometry of these nanoscale electrochemical probes. Due to the size and the site-specific directed synthesis of the VACNFs, these probes are ideally suited for characterizing electrochemical phenomena with an unprecedented degree of spatial resolution.

Test module development to detect the flase call probe pins on microeprocessor test equipment

NASA Astrophysics Data System (ADS)

Tang, L. W.; Ong, N. R.; Mohamad, I. S. B.; Alcain, J. B.; Retnasamy, V.

2017-09-01

Probe pins are useful for electrical testing of microelectronic components, printed circuit board assembly (PCBA), microprocessors and other electronic devices due to it provides the conductivity test based on specific device circuit design. During the repeatable test runs, the load of test modules, contact failures and the current conductivity induces layer wear off all the tip of probe pins contact. Contamination will be build-up on probe pins and increased contact resistivity which results of cost loss and time loss for rectifying programs, rectifying testers and exchanging new probe pins. In this study, a resistivity approach will be developed to provide "Testing of Test Probes". The test module based on "Four-wire Ohm measurement" method with two alternative ways of applying power supply, that are 9V from a single power supply and 5V from Arduino UNO power supply were demonstrated to measure the small resistance value of microprocessor probe pin. A microcontroller with VEE Pro software was used to record the measurement data. The accuracy of both test modules were calibrated under different temperature conditions and result shows that 9V from a single power supply test module has higher measurement accuracy.

Evaluation of a Magnetically-Filtered Faraday Probe for Measuring the ion Current Density Profile of a Hall Thruster

DTIC Science & Technology

2004-07-01

The ability of a magnetically-filtered Faraday probe (MFFP) to obtain the ion current density profile of a Hall thruster is investigated. The MFFP is...MFFP, boxed Faraday probe (BFP), and nude Faraday probe are used to measure the ion current density profile of a 5 kW Hall thruster operated over the

Non-invasive probe diagnostic method for electron temperature and ion current density in atmospheric pressure plasma jet source

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kim, Young-Cheol; Kim, Yu-Sin; Lee, Hyo-Chang

2015-08-15

The electrical probe diagnostics are very hard to be applied to atmospheric plasmas due to severe perturbation by the electrical probes. To overcome this, the probe for measuring electron temperature and ion current density is indirectly contacted with an atmospheric jet source. The plasma parameters are obtained by using floating harmonic analysis. The probe is mounted on the quartz tube that surrounds plasma. When a sinusoidal voltage is applied to a probe contacting on a quartz tube, the electrons near the sheath at dielectric tube are collected and the probe current has harmonic components due to probe sheath nonlinearity. Frommore » the relation of the harmonic currents and amplitude of the sheath voltage, the electron temperature near the wall can be obtained with collisional sheath model. The electron temperatures and ion current densities measured at the discharge region are in the ranges of 2.7–3.4 eV and 1.7–5.2 mA/cm{sup 2} at various flow rates and input powers.« less

Renilla luciferase-labeled Annexin V: a new probe for detection of apoptotic cells.

PubMed

Nazari, Mahboobeh; Emamzadeh, Rahman; Hosseinkhani, Saman; Cevenini, Luca; Michelini, Elisa; Roda, Aldo

2012-11-07

The Ca(2+)-dependent binding of Annexin V to phosphatidylserine on cell surfaces is a reliable marker for apoptosis that is widely used in flow cytometry based apoptosis assays. In this paper, we report a new class of Annexin V-based probes for apoptosis. Luciferase from Renilla reniformis (RLuc) was linked to Annexin V and expressed successfully in a soluble form in Escherichia coli BL21 (DE3). The new probe, Rluc/Annexin V, was purified and functionally assayed for detection of apoptosis in actinomycin D-induced apoptotic Jurkat cells. Moreover, the spontaneous apoptosis in neutrophils was shown using the new probe. The results indicate that Rluc/Annexin V can bind to the apoptotic cells, and the signal of Renilla luciferase can be detected by luminometric measurements. The availability of Rluc/Annexin V may be of potential commercial interest for improving current apoptosis assays.

Design and Application of Hybrid Magnetic Field-Eddy Current Probe

NASA Technical Reports Server (NTRS)

Wincheski, Buzz; Wallace, Terryl; Newman, Andy; Leser, Paul; Simpson, John

2013-01-01

The incorporation of magnetic field sensors into eddy current probes can result in novel probe designs with unique performance characteristics. One such example is a recently developed electromagnetic probe consisting of a two-channel magnetoresistive sensor with an embedded single-strand eddy current inducer. Magnetic flux leakage maps of ferrous materials are generated from the DC sensor response while high-resolution eddy current imaging is simultaneously performed at frequencies up to 5 megahertz. In this work the design and optimization of this probe will be presented, along with an application toward analysis of sensory materials with embedded ferromagnetic shape-memory alloy (FSMA) particles. The sensory material is designed to produce a paramagnetic to ferromagnetic transition in the FSMA particles under strain. Mapping of the stray magnetic field and eddy current response of the sample with the hybrid probe can thereby image locations in the structure which have experienced an overstrain condition. Numerical modeling of the probe response is performed with good agreement with experimental results.

Apparatus for measuring high frequency currents

NASA Technical Reports Server (NTRS)

Hagmann, Mark J. (Inventor); Sutton, John F. (Inventor)

2003-01-01

An apparatus for measuring high frequency currents includes a non-ferrous core current probe that is coupled to a wide-band transimpedance amplifier. The current probe has a secondary winding with a winding resistance that is substantially smaller than the reactance of the winding. The sensitivity of the current probe is substantially flat over a wide band of frequencies. The apparatus is particularly useful for measuring exposure of humans to radio frequency currents.

Flame exposure time on Langmuir probe degradation, ion density, and thermionic emission for flame temperature.

PubMed

Doyle, S J; Salvador, P R; Xu, K G

2017-11-01

The paper examines the effect of exposure time of Langmuir probes in an atmospheric premixed methane-air flame. The effects of probe size and material composition on current measurements were investigated, with molybdenum and tungsten probe tips ranging in diameter from 0.0508 to 0.1651 mm. Repeated prolonged exposures to the flame, with five runs of 60 s, resulted in gradual probe degradations (-6% to -62% area loss) which affected the measurements. Due to long flame exposures, two ion saturation currents were observed, resulting in significantly different ion densities ranging from 1.16 × 10 16 to 2.71 × 10 19 m -3 . The difference between the saturation currents is caused by thermionic emissions from the probe tip. As thermionic emission is temperature dependent, the flame temperature could thus be estimated from the change in current. The flame temperatures calculated from the difference in saturation currents (1734-1887 K) were compared to those from a conventional thermocouple (1580-1908 K). Temperature measurements obtained from tungsten probes placed in rich flames yielded the highest percent error (9.66%-18.70%) due to smaller emission current densities at lower temperatures. The molybdenum probe yielded an accurate temperature value with only 1.29% error. Molybdenum also demonstrated very low probe degradation in comparison to the tungsten probe tips (area reductions of 6% vs. 58%, respectively). The results also show that very little exposure time (<5 s) is needed to obtain a valid ion density measurement and that prolonged flame exposures can yield the flame temperature but also risks damage to the Langmuir probe tip.

Silica-gold nanoshells as potential intraoperative molecular probes for HER2-overexpression in ex vivo breast tissue using near-infrared reflectance confocal microscopy.

PubMed

Bickford, Lissett R; Agollah, Germaine; Drezek, Rebekah; Yu, Tse-Kuan

2010-04-01

Obtaining negative margins is critical for breast cancer patients undergoing conservation therapy in order to reduce the reemergence of the original cancer. Currently, breast cancer tumor margins are examined in a pathology lab either while the patient is anesthetized or after the surgical procedure has been terminated. These current methods often result in cancer cells present at the surgical resection margin due to inadequate margin assessment at the point of care. Due to such limitations evident in current diagnoses, tools for increasing the accuracy and speed of tumor margin detection directly in the operating room are still needed. We are exploring the potential of using a nano-biophotonics system to facilitate intraoperative tumor margin assessment ex vivo at the cellular level. By combining bioconjugated silica-based gold nanoshells, which scatter light in the near-infrared, with a portable FDA-approved reflectance confocal microscope, we first validate the use of gold nanoshells as effective reflectance-based imaging probes by evaluating the contrast enhancement of three different HER2-overexpressing cell lines. Additionally, we demonstrate the ability to detect HER2-overexpressing cells in human tissue sections within 5 min of incubation time. This work supports the use of targeted silica-based gold nanoshells as potential real-time molecular probes for HER2-overexpression in human tissue.

Behavior of Triple Langmuir Probes in Non-Equilibrium Plasmas

NASA Technical Reports Server (NTRS)

Polzin, Kurt A.; Ratcliffe, Alicia C.

2018-01-01

The triple Langmuir probe is an electrostatic probe in which three probe tips collect current when inserted into a plasma. The triple probe differs from a simple single Langmuir probe in the nature of the voltage applied to the probe tips. In the single probe, a swept voltage is applied to the probe tip to acquire a waveform showing the collected current as a function of applied voltage (I-V curve). In a triple probe three probe tips are electrically coupled to each other with constant voltages applied between each of the tips. The voltages are selected such that they would represent three points on the single Langmuir probe I-V curve. Elimination of the voltage sweep makes it possible to measure time-varying plasma properties in transient plasmas. Under the assumption of a Maxwellian plasma, one can determine the time-varying plasma temperature T(sub e)(t) and number density n(sub e)(t) from the applied voltage levels and the time-histories of the collected currents. In the present paper we examine the theory of triple probe operation, specifically focusing on the assumption of a Maxwellian plasma. Triple probe measurements have been widely employed for a number of pulsed and timevarying plasmas, including pulsed plasma thrusters (PPTs), dense plasma focus devices, plasma flows, and fusion experiments. While the equilibrium assumption may be justified for some applications, it is unlikely that it is fully justifiable for all pulsed and time-varying plasmas or for all times during the pulse of a plasma device. To examine a simple non-equilibrium plasma case, we return to basic governing equations of probe current collection and compute the current to the probes for a distribution function consisting of two Maxwellian distributions with different temperatures (the two-temperature Maxwellian). A variation of this method is also employed, where one of the Maxwellians is offset from zero (in velocity space) to add a suprathermal beam of electrons to the tail of the main Maxwellian distribution (the bump-on-the-tail distribution function). For a range of parameters in these non-Maxwellian distributions, we compute the current collection to the probes. We compare the distribution function that was assumed a priori with the distribution function one would infer when applying standard triple probe theory to analyze the collected currents. For the assumed class of non-Maxwellian distribution functions this serves to illustrate the effect a non-Maxwellian plasma would have on results interpreted using the equilibrium triple probe current collection theory, allowing us to state the magnitudes of these deviations as a function of the assumed distribution function properties.

Technique for temperature compensation of eddy-current proximity probes

NASA Technical Reports Server (NTRS)

Masters, Robert M.

1989-01-01

Eddy-current proximity probes are used in turbomachinery evaluation testing and operation to measure distances, primarily vibration, deflection, or displacment of shafts, bearings and seals. Measurements of steady-state conditions made with standard eddy-current proximity probes are susceptible to error caused by temperature variations during normal operation of the component under investigation. Errors resulting from temperature effects for the specific probes used in this study were approximately 1.016 x 10 to the -3 mm/deg C over the temperature range of -252 to 100 C. This report examines temperature caused changes on the eddy-current proximity probe measurement system, establishes their origin, and discusses what may be done to minimize their effect on the output signal. In addition, recommendations are made for the installation and operation of the electronic components associated with an eddy-current proximity probe. Several techniques are described that provide active on-line error compensation for over 95 percent of the temperature effects.

Design and test of voltage and current probes for EAST ICRF antenna impedance measurement

NASA Astrophysics Data System (ADS)

Jianhua, WANG; Gen, CHEN; Yanping, ZHAO; Yuzhou, MAO; Shuai, YUAN; Xinjun, ZHANG; Hua, YANG; Chengming, QIN; Yan, CHENG; Yuqing, YANG; Guillaume, URBANCZYK; Lunan, LIU; Jian, CHENG

2018-04-01

On the experimental advanced superconducting tokamak (EAST), a pair of voltage and current probes (V/I probes) is installed on the ion cyclotron radio frequency transmission lines to measure the antenna input impedance, and supplement the conventional measurement technique based on voltage probe arrays. The coupling coefficients of V/I probes are sensitive to their sizes and installing locations, thus they should be determined properly to match the measurement range of data acquisition card. The V/I probes are tested in a testing platform at low power with various artificial loads. The testing results show that the deviation of coupling resistance is small for loads R L > 2.5 Ω, while the resistance deviations appear large for loads R L < 1.5 Ω, which implies that the power loss cannot be neglected at high VSWR. As the factors that give rise to the deviation of coupling resistance calculation, the phase measurement error is the more significant factor leads to deleterious results rather than the amplitude measurement error. To exclude the possible ingredients that may lead to phase measurement error, the phase detector can be calibrated in steady L-mode scenario and then use the calibrated data for calculation under H-mode cases in EAST experiments.

Molecular Imaging Probe Development using Microfluidics

PubMed Central

Liu, Kan; Wang, Ming-Wei; Lin, Wei-Yu; Phung, Duy Linh; Girgis, Mark D.; Wu, Anna M.; Tomlinson, James S.; Shen, Clifton K.-F.

2012-01-01

In this manuscript, we review the latest advancement of microfluidics in molecular imaging probe development. Due to increasing needs for medical imaging, high demand for many types of molecular imaging probes will have to be met by exploiting novel chemistry/radiochemistry and engineering technologies to improve the production and development of suitable probes. The microfluidic-based probe synthesis is currently attracting a great deal of interest because of their potential to deliver many advantages over conventional systems. Numerous chemical reactions have been successfully performed in micro-reactors and the results convincingly demonstrate with great benefits to aid synthetic procedures, such as purer products, higher yields, shorter reaction times compared to the corresponding batch/macroscale reactions, and more benign reaction conditions. Several ‘proof-of-principle’ examples of molecular imaging probe syntheses using microfluidics, along with basics of device architecture and operation, and their potential limitations are discussed here. PMID:22977436

A compact, smart Langmuir Probe control module for MAST-Upgrade

NASA Astrophysics Data System (ADS)

Lovell, J.; Stephen, R.; Bray, S.; Naylor, G.; Elmore, S.; Willett, H.; Peterka, M.; Dimitrova, M.; Havranek, A.; Hron, M.; Sharples, R.

2017-11-01

A new control module for the MAST-Upgrade Langmuir Probe system has been developed. It is based on a Xilinx Zynq FPGA, which allows for excellent configurability and ease of retrieving data. The module is capable of arbitrary bias voltage waveform generation, and digitises current and voltage readings from 16 probes. The probes are biased and measured one at a time in a time multiplexed fashion, with the multiplexing sequence completely configurable. In addition, simultaneous digitisation of the floating potential of all unbiased probes is possible. A suite of these modules, each coupled with a high voltage amplifier, enables biasing and digitisation of 640 Langmuir Probes in the MAST-Upgrade Super-X divertor. The system has been successfully tested on the York Linear Plasma Device and on the COMPASS tokamak. It will be installed on MAST-Upgrade ready for operations in 2018.

Eddy current probe with foil sensor mounted on flexible probe tip and method of use

DOEpatents

Viertl, John R. M.; Lee, Martin K.

2001-01-01

A pair of copper coils are embedded in the foil strip. A first coil of the pair generates an electromagnetic field that induces eddy currents on the surface, and the second coil carries a current influenced by the eddy currents on the surface. The currents in the second coil are analyzed to obtain information on the surface eddy currents. An eddy current probe has a metal housing having a tip that is covered by a flexible conductive foil strip. The foil strip is mounted on a deformable nose at the probe tip so that the strip and coils will conform to the surface to which they are applied.

Evaluation of Plume Divergence and Facility Effects on Far-Field Faraday Probe Current Density Profiles

DTIC Science & Technology

2009-09-01

elevated background pressure, compared nude Faraday probe designs, and evaluated design modifications to minimize uncertainty due to charge exchange...evaluated Faraday probe design and facility background pressure on collected ion current. A comparison of two nude Faraday probe designs concluded...140.5 Plasma potential in the region surrounding a nude Faraday probe has been measured to study the possibility of probe bias voltage acting as a

Bunker probe: A plasma potential probe almost insensitive to its orientation with the magnetic field

DOE Office of Scientific and Technical Information (OSTI.GOV)

Costea, S., E-mail: stefan.costea@uibk.ac.at; Schneider, B. S.; Schrittwieser, R.

Due to their ability to suppress a large part of the electron current and thus measuring directly the plasma potential, ion sensitive probes have begun to be widely tested and used in fusion devices. For these probes to work, almost perfect alignment with the total magnetic field is necessary. This condition cannot always be fulfilled due to the curvature of magnetic fields, complex magnetic structure, or magnetic field reconnection. In this perspective, we have developed a plasma potential probe (named Bunker probe) based on the principle of the ion sensitive probe but almost insensitive to its orientation with the totalmore » magnetic field. Therefore it can be used to measure the plasma potential inside fusion devices, especially in regions with complex magnetic field topology. Experimental results are presented and compared with Ball-Pen probe measurements taken under identical conditions. We have observed that the floating potential of the Bunker probe is indeed little affected by its orientation with the magnetic field for angles ranging from 90° to 30°, in contrast to the Ball-Pen probe whose floating potential decreases towards that of a Langmuir probe if not properly aligned with the magnetic field.« less

Development of Eddy Current Techniques for Detection of Deep Fatigue Cracks in Multi-Layer Airframe Components

NASA Technical Reports Server (NTRS)

Wincheski, Russell A.

2008-01-01

Thick, multi-layer aluminum structure has been widely used in aircraft design in critical wing splice areas. The multi-layer structure generally consists of three or four aluminum layers with different geometry and varying thickness, which are held together with fasteners. The detection of cracks under fasteners with ultrasonic techniques in subsurface layers away from the skin is impeded primarily by interlayer bonds and faying sealant condition. Further, assessment of such sealant condition is extremely challenging in terms of complexity of structure, limited access, and inspection cost. Although Eddy current techniques can be applied on in-service aircraft from the exterior of the skin without knowing sealant condition, the current eddy current techniques are not able to detect defects with wanted sensitivity. In this work a series of low frequency eddy current probes have been designed, fabricated and tested for this application. A probe design incorporating a shielded magnetic field sensor concentrically located in the interior of a drive coil has been employed to enable a localized deep diffusion of the electromagnetic field into the part under test. Due to the required low frequency inspections, probes have been testing using a variety of magnetic field sensors (pickup coil, giant magneto-resistive, anisotropic magneto-resistive, and spin-dependent tunneling). The probe designs as well as capabilities based upon a target inspection for sub-layer cracking in an airframe wing spar joint is presented.

Note: Measurement of the cathode layer thickness in glow discharges with a Langmuir probe

NASA Astrophysics Data System (ADS)

Wang, Hao; Hou, Xinyu; Zou, Xiaobing; Luo, Haiyun; Wang, Xinxin

2018-06-01

A method using a Langmuir probe to determine the thickness of the cathode layer for a glow discharge is developed. The method is based on the phenomenon that the curve of the voltage-current characteristics changes in shape as the Langmuir probe moves from the positive column into the cathode layer. The method was used to measure the thicknesses of the cathode layer in the normal glow discharges of argon and air with the cathodes made from stainless steel and aluminum. The results are in good agreement with those given in a book of gas discharge.

Biomimetic nanochannels based biosensor for ultrasensitive and label-free detection of nucleic acids.

PubMed

Sun, Zhongyue; Liao, Tangbin; Zhang, Yulin; Shu, Jing; Zhang, Hong; Zhang, Guo-Jun

2016-12-15

A very simple sensing device based on biomimetic nanochannels has been developed for label-free, ultrasensitive and highly sequence-specific detection of DNA. Probe DNA was modified on the inner wall of the nanochannel surface by layer-by-layer (LBL) assembly. After probe DNA immobilization, DNA detection was realized by monitoring the rectified ion current when hybridization occurred. Due to three dimensional (3D) nanoscale environment of the nanochannel, this special geometry dramatically increased the surface area of the nanochannel for immobilization of probe molecules on the inner-surface and enlarged contact area between probes and target-molecules. Thus, the unique sensor reached a reliable detection limit of 10 fM for target DNA. In addition, this DNA sensor could discriminate complementary DNA (c-DNA) from non-complementary DNA (nc-DNA), two-base mismatched DNA (2bm-DNA) and one-base mismatched DNA (1bm-DNA) with high specificity. Moreover, the nanochannel-based biosensor was also able to detect target DNA even in an interfering environment and serum samples. This approach will provide a novel biosensing platform for detection and discrimination of disease-related molecular targets and unknown sequence DNA. Copyright © 2016 Elsevier B.V. All rights reserved.

Electronic thermometry in tunable tunnel junction

DOE Office of Scientific and Technical Information (OSTI.GOV)

Maksymovych, Petro

A tunable tunnel junction thermometry circuit includes a variable width tunnel junction between a test object and a probe. The junction width is varied and a change in thermovoltage across the junction with respect to the change in distance across the junction is determined. Also, a change in biased current with respect to a change in distance across the junction is determined. A temperature gradient across the junction is determined based on a mathematical relationship between the temperature gradient, the change in thermovoltage with respect to distance and the change in biased current with respect to distance. Thermovoltage may bemore » measured by nullifying a thermoelectric tunneling current with an applied voltage supply level. A piezoelectric actuator may modulate the probe, and thus the junction width, to vary thermovoltage and biased current across the junction. Lock-in amplifiers measure the derivatives of the thermovoltage and biased current modulated by varying junction width.« less

Langmuir probe diagnostics of an atmospheric pressure, vortex-stabilized nitrogen plasma jet

DOE Office of Scientific and Technical Information (OSTI.GOV)

Prevosto, L.; Mancinelli, B. R.; Kelly, H.

Langmuir probe measurements in an atmospheric pressure direct current (dc) plasma jet are reported. Sweeping probes were used. The experiment was carried out using a dc non-transferred arc torch with a rod-type cathode and an anode of 5 mm diameter. The torch was operated at a nominal power level of 15 kW with a nitrogen flow rate of 25 Nl min{sup -1}. A flat ion saturation region was found in the current-voltage curve of the probe. The ion saturation current to a cylindrical probe in a high-pressure non local thermal equilibrium (LTE) plasma was modeled. Thermal effects and ionization/recombination processesmore » inside the probe perturbed region were taken into account. Averaged radial profiles of the electron and heavy particle temperatures as well as the electron density were obtained. An electron temperature around 11 000 K, a heavy particle temperature around 9500 K and an electron density of about 4 Multiplication-Sign 10{sup 22} m{sup -3}, were found at the jet centre at 3.5 mm downstream from the torch exit. Large deviations from kinetic equilibrium were found throughout the plasma jet. The electron and heavy particle temperature profiles showed good agreement with those reported in the literature by using spectroscopic techniques. It was also found that the temperature radial profile based on LTE was very close to that of the electrons. The calculations have shown that this method is particularly useful for studying spraying-type plasma jets characterized by electron temperatures in the range 9000-14 000 K.« less

An analytical model for floating probes in AC plasma and its application to double probes for high density, high power RF discharges

NASA Astrophysics Data System (ADS)

Caneses, Juan Francisco; Blackwell, Boyd; Plasma Research Laboratory Team

2013-10-01

In this work we provide an analytical model that allows one to quantitatively assess the RF compensation performance and suitability of the double probe technique for use in RF generated plasma. The model is based in the theory of the self-bias effect as described in Braithwaite's work, which we extend to include the time resolved behavior of floating probes. We provide experimental verification for this model and show that the theory of transient RF self-bias probes and harmonic current detection probes are limiting cases of this extended model. Furthermore, the model shows that the RF compensation is solely dependent on the sheath impedance, the probe's stray capacitance to ground and RF frequency. In addition, we use these results to implement a double probe system for use in high density helicon plasma where heat loads could potentially damage the intricate components in an RF compensating circuit. Finally we use this model to (1) recommend ways to extend the operational regime of double probes where the plasma conditions would render them unsuitable and to (2) comment on the use of this model to aid design of RF compensated Langmuir probes.

Species-specific identification of Dekkera/Brettanomyces yeasts by fluorescently labeled DNA probes targeting the 26S rRNA.

PubMed

Röder, Christoph; König, Helmut; Fröhlich, Jürgen

2007-09-01

Sequencing of the complete 26S rRNA genes of all Dekkera/Brettanomyces species colonizing different beverages revealed the potential for a specific primer and probe design to support diagnostic PCR approaches and FISH. By analysis of the complete 26S rRNA genes of all five currently known Dekkera/Brettanomyces species (Dekkera bruxellensis, D. anomala, Brettanomyces custersianus, B. nanus and B. naardenensis), several regions with high nucleotide sequence variability yet distinct from the D1/D2 domains were identified. FISH species-specific probes targeting the 26S rRNA gene's most variable regions were designed. Accessibility of probe targets for hybridization was facilitated by the construction of partially complementary 'side'-labeled probes, based on secondary structure models of the rRNA sequences. The specificity and routine applicability of the FISH-based method for yeast identification were tested by analyzing different wine isolates. Investigation of the prevalence of Dekkera/Brettanomyces yeasts in the German viticultural regions Wonnegau, Nierstein and Bingen (Rhinehesse, Rhineland-Palatinate) resulted in the isolation of 37 D. bruxellensis strains from 291 wine samples.

In Situ Live-Cell Nucleus Fluorescence Labeling with Bioinspired Fluorescent Probes.

PubMed

Ding, Pan; Wang, Houyu; Song, Bin; Ji, Xiaoyuan; Su, Yuanyuan; He, Yao

2017-08-01

Fluorescent imaging techniques for visualization of nuclear structure and function in live cells are fundamentally important for exploring major cellular events. The ideal cellular labeling method is capable of realizing label-free, in situ, real-time, and long-term nucleus labeling in live cells, which can fully obtain the nucleus-relative information and effectively alleviate negative effects of alien probes on cellular metabolism. However, current established fluorescent probes-based strategies (e.g., fluorescent proteins-, organic dyes-, fluorescent organic/inorganic nanoparticles-based imaging techniques) are unable to simultaneously realize label-free, in situ, long-term, and real-time nucleus labeling, resulting in inevitable difficulties in fully visualizing nuclear structure and function in live cells. To this end, we present a type of bioinspired fluorescent probes, which are highly efficacious for in situ and label-free tracking of nucleus in long-term and real-time manners. Typically, the bioinspired polydopamine (PDA) nanoparticles, served as fluorescent probes, can be readily synthesized in situ within live cell nucleus without any further modifications under physiological conditions (37 °C, pH ∼7.4). Compared with other conventional nuclear dyes (e.g., propidium iodide (PI), Hoechst), superior spectroscopic properties (e.g., quantum yield of ∼35.8% and high photostability) and low cytotoxicity of PDA-based probes enable long-term (e.g., 3 h) fluorescence tracking of nucleus. We also demonstrate the generality of this type of bioinspired fluorescent probes in different cell lines and complex biological samples.

Eddy-Current Inspection of Ball Bearings

NASA Technical Reports Server (NTRS)

Bankston, B.

1985-01-01

Custom eddy-current probe locates surface anomalies. Low friction air cushion within cone allows ball to roll easily. Eddy current probe reliably detects surface and near-surface cracks, voids, and material anomalies in bearing balls or other spherical objects. Defects in ball surface detected by probe displayed on CRT and recorded on strip-chart recorder.

Enzyme-less electrochemical displacement heterogeneous immunosensor for diclofenac detection.

PubMed

Nguyen, T T K; Vu, T T; Anquetin, G; Tran, H V; Reisberg, S; Noël, V; Mattana, G; Nguyen, Q V; Dai Lam, Tran; Pham, M C; Piro, B

2017-11-15

We describe an electrochemical immunosensor based on functionalization of a working electrode by electrografting two functional diazonium salts. The first one is a molecular probe, diclofenac, coupled with an arylamine onto which a specific antibody is immobilized by affinity interactions; the second is a redox probe (a quinone) also coupled with an arylamine, able to transduce the hapten-antibody association into a change in electroactivity. The steric hindrance induced by the antibody leads to a current decrease upon binding of the antibody on the grafted molecular probe; conversely, when diclofenac is present in solution, a displacement equilibrium occurs between the target diffusing into the solution and the grafted probe. This leads to dissociation of the antibody from the electrode surface, event which is transduced into a current increase ("signal-on" detection). The detection limit is ca. 20 fM, corresponding to 6pgL -1 diclofenac, which is competitive compared to other label-free immunosensors. We demonstrate that the sensor is selective and is able to quantify diclofenac in tap water. Copyright © 2017 Elsevier B.V. All rights reserved.

Analysis of wall-embedded Langmuir probe signals in different conditions on the Tokamak à Configuration Variable

NASA Astrophysics Data System (ADS)

Février, O.; Theiler, C.; De Oliveira, H.; Labit, B.; Fedorczak, N.; Baillod, A.

2018-05-01

This paper presents the current wall-embedded Langmuir probe system installed on the Tokamak à Configuration Variable (TCV), as well as the analysis tool chain used to interpret the current-voltage characteristic obtained when the probes are operated in swept-bias conditions. The analysis is based on a four-parameter fit combined with a minimum temperature approach. In order to reduce the effect of plasma fluctuations and measurement noise, several current-voltage characteristics are usually averaged before proceeding to the fitting. The impact of this procedure on the results is investigated, as well as the possible role of finite resistances in the circuitry, which could lead to an overestimation of the temperature. We study the application of the procedure in a specific regime, the plasma detachment, where results from other diagnostics indicate that the electron temperature derived from the Langmuir probes might be overestimated. To address this issue, we explore other fitting models and, in particular, an extension of the asymmetric double probe fit, which features effects of sheath expansion. We show that these models yield lower temperatures (up to approximately 60%) than the standard analysis in detached conditions, particularly for a temperature peak observed near the plasma strike point, but a discrepancy with other measurements remains. We explore a possible explanation for this observation, the presence of a fast electron population, and assess how robust the different methods are in such conditions.

Experimental Studies of the Effect of Intent Information on Cockpit Traffic Displays

NASA Technical Reports Server (NTRS)

Barhydt, Richard; Hansman, R. John

1997-01-01

Intent information provides knowledge of another aircraft's current and future trajectory states. Prototype traffic displays were designed for four different levels of intent: Position, Rate, Commanded State, and FMS (Flight Management System)-Path. The current TCAS (traffic collision avoidance systems) Display, which shows altitude rate in addition to current position and altitude, was used as a baseline and represents the lowest level of intent. The Rate, Commanded State, and FMS-Path Displays show increasing levels of intent information using TCAS-like symbology in addition to incorporating a conflict probe and profile view display. An initial experiment was run on the MIT (Massachusetts Institute of Technology) Part Task Flight Simulator in which eight airline pilots flew five traffic scenarios with each of the four displays. Results show that pilots had fewer separation violations and maneuvered earlier with the three intent displays. Separation violations were reduced when pilots maneuvered earlier. A second experiment was run to compare performance between displaying intent information directly and incorporating it into a conflict probe. A different set of eight airline pilots flew four traffic scenarios with the TCAS and Commanded State Displays with and without the conflict probe. Conflict probes with two minute and long range look-ahead times were tested. Displaying conflict bands or showing intent information directly both led to fewer separation violations and earlier avoidance maneuvers than the base TCAS Display. Performance was similar between the two minute and long range look-ahead conflict probes. Pilots preferred all intent displays over the TCAS Display.

Correcting magnetic probe perturbations on current density measurements of current carrying plasmas.

PubMed

Knoblauch, P; Raspa, V; Di Lorenzo, F; Lazarte, A; Clausse, A; Moreno, C

2010-09-01

A method to infer the current density distribution in the current sheath of a plasma focus discharge from a magnetic probe is formulated and then applied to experimental data obtained in a 1.1 kJ device. Distortions on the magnetic probe signal caused by current redistribution and by a time-dependent total discharge current are considered simultaneously, leading to an integral equation for the current density. Two distinct, easy to implement, numerical procedures are given to solve such equation. Experimental results show the coexistence of at least two maxima in the current density structure of a nitrogen sheath.

A branch-migration based fluorescent probe for straightforward, sensitive and specific discrimination of DNA mutations

PubMed Central

Xiao, Xianjin; Wu, Tongbo; Xu, Lei; Chen, Wei

2017-01-01

Abstract Genetic mutations are important biomarkers for cancer diagnostics and surveillance. Preferably, the methods for mutation detection should be straightforward, highly specific and sensitive to low-level mutations within various sequence contexts, fast and applicable at room-temperature. Though some of the currently available methods have shown very encouraging results, their discrimination efficiency is still very low. Herein, we demonstrate a branch-migration based fluorescent probe (BM probe) which is able to identify the presence of known or unknown single-base variations at abundances down to 0.3%-1% within 5 min, even in highly GC-rich sequence regions. The discrimination factors between the perfect-match target and single-base mismatched target are determined to be 89–311 by measurement of their respective branch-migration products via polymerase elongation reactions. The BM probe not only enabled sensitive detection of two types of EGFR-associated point mutations located in GC-rich regions, but also successfully identified the BRAF V600E mutation in the serum from a thyroid cancer patient which could not be detected by the conventional sequencing method. The new method would be an ideal choice for high-throughput in vitro diagnostics and precise clinical treatment. PMID:28201758

Noncontact Measurement Of Sizes And Eccentricities Of Holes

NASA Technical Reports Server (NTRS)

Chern, Engmin J.

1993-01-01

Semiautomatic eddy-current-probe apparatus makes noncontact measurements of nominally round holes in electrically conductive specimens and processes measurement data into diameters and eccentricities of holes. Includes x-y translation platform, which holds specimen and moves it horizontally. Probe mounted on probe scanner, positioning probe along vertical (z) direction and rotates probe about vertical axis at preset low speed. Eddy-current sensing coil mounted in side of probe near tip. As probe rotates, impedance analyzer measures electrical impedance (Z) of coil as function of instantaneous rotation angle. Translation and rotation mechanisms and impedance analyzer controlled by computer, which also processes impedance-measurement data.

Nucleic acid probes in diagnostic medicine

NASA Technical Reports Server (NTRS)

Oberry, Phillip A.

1991-01-01

The need for improved diagnostic procedures is outlined and variations in probe technology are briefly reviewed. A discussion of the application of probe technology to the diagnosis of disease in animals and humans is presented. A comparison of probe versus nonprobe diagnostics and isotopic versus nonisotopic probes is made and the current state of sequence amplification is described. The current market status of nucleic acid probes is reviewed with respect to their diagnostic application in human and veterinary medicine. Representative product examples are described and information on probes being developed that offer promise as future products is discussed.

Effects of pipette modulation and imaging distances on ion currents measured with scanning ion conductance microscopy (SICM).

PubMed

Chen, Chiao-Chen; Baker, Lane A

2011-01-07

Local conductance variations can be estimated by measuring ion current magnitudes with scanning ion conductance microscopy (SICM). Factors which influence image quality and quantitation of ion currents measured with SICM have been evaluated. Specifically, effects of probe-sample separation and pipette modulation have been systematically studied for the case of imaging conductance variations at pores in a polymer membrane under transmembrane concentration gradients. The influence of probe-sample separation on ion current images was evaluated using distance-modulated (ac) feedback. Approach curves obtained using non-modulated (dc) feedback were also recorded to determine the relative influence of pipette-generated convection by comparison of ion currents measured with both ac and dc feedback modes. To better interpret results obtained, comparison to a model based on a disk-shaped geometry for nanopores in the membrane, as well as relevant position-dependent parameters of the experiment is described. These results advance our current understanding of conductance measurements with SICM.

Fuel cell CO sensor

DOEpatents

Grot, Stephen Andreas; Meltser, Mark Alexander; Gutowski, Stanley; Neutzler, Jay Kevin; Borup, Rodney Lynn; Weisbrod, Kirk

1999-12-14

The CO concentration in the H.sub.2 feed stream to a PEM fuel cell stack is monitored by measuring current and/or voltage behavior patterns from a PEM-probe communicating with the reformate feed stream. Pattern recognition software may be used to compare the current and voltage patterns from the PEM-probe to current and voltage telltale outputs determined from a reference cell similar to the PEM-probe and operated under controlled conditions over a wide range of CO concentrations in the H.sub.2 fuel stream. A CO sensor includes the PEM-probe, an electrical discharge circuit for discharging the PEM-probe to monitor the CO concentration, and an electrical purging circuit to intermittently raise the anode potential of the PEM-probe's anode to at least about 0.8 V (RHE) to electrochemically oxidize any CO adsorbed on the probe's anode catalyst.

Method of monitoring CO concentrations in hydrogen feed to a PEM fuel cell

DOEpatents

Grot, Stephen Andreas; Meltser, Mark Alexander; Gutowski, Stanley; Neutzler, Jay Kevin; Borup, Rodney Lynn; Weisbrod, Kirk

2000-01-01

The CO concentration in the H.sub.2 feed stream to a PEM fuel cell stack is monitored by measuring current and/or voltage behavior patterns from a PEM-probe communicating with the reformate feed stream. Pattern recognition software may be used to compare the current and voltage patterns from the PEM-probe to current and voltage telltale outputs determined from a reference cell similar to the PEM-probe and operated under controlled conditions over a wide range of CO concentrations in the H.sub.2 fuel stream. The PEM-probe is intermittently purged of any CO build-up on the anode catalyst (e.g., by (1) flushing the anode with air, (2) short circuiting the PEM-probe, or (3) reverse biasing the PEM-probe) to keep the PEM-probe at peak performance levels.

Highly sensitive current sensor based on an optical microfiber loop resonator incorporating low index polymer

NASA Astrophysics Data System (ADS)

Yoon, Min-Seok; Han, Young-Geun

2014-05-01

A highly sensitive current sensor based on an optical microfiber loop resonator (MLR) incorporating low index polymer is proposed and experimentally demonstrated. The microfiber with a waist diameter of 1 μm is wrapped around the nicrhrome wire with low index polymer coating and the optical MLR is realized. The use of the microfiber and low index polymer with high thermal property can effectively improve the current sensitivity of the proposed MLR-based sensing probe to be 437.9 pm/A2, which is ~10 times higher than the previous result.

Subclass-specific labeling of protein-reactive natural products with customized nucleophilic probes.

PubMed

Rudolf, Georg C; Koch, Maximilian F; Mandl, Franziska A M; Sieber, Stephan A

2015-02-23

Natural products represent a rich source of bioactive compounds that constitute a large fraction of approved drugs. Among those are molecules with electrophilic scaffolds, such as Michael acceptors, β-lactams, and epoxides that irreversibly inhibit essential enzymes based on their catalytic mechanism. In the search for novel bioactive molecules, current methods are challenged by the frequent rediscovery of known chemical entities. Herein small nucleophilic probes that attack electrophilic natural products and enhance their detection by HPLC-UV and HPLC-MS are introduced. A screen of diverse probe designs revealed one compound with a desired selectivity for epoxide- and maleimide-based antibiotics. Correspondingly, the natural products showdomycin and phosphomycin could be selectively targeted in extracts of their natural producing organism, in which the probe-modified molecules exhibited superior retention and MS detection relative to their unmodified counterparts. This method may thus help to discover small, electrophilic molecules that might otherwise easily elude detection in complex samples. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Magnetic dipole discharges. II. Cathode and anode spot discharges and probe diagnostics

NASA Astrophysics Data System (ADS)

Stenzel, R. L.; Urrutia, J. M.; Ionita, C.; Schrittwieser, R.

2013-08-01

The high current regime of a magnetron-type discharge has been investigated. The discharge uses a permanent magnet as a cold cathode which emits secondary electrons while the chamber wall or a grounded electrode serves as the anode. As the discharge voltage is increased, the magnet develops cathode spots, which are short duration arcs that provide copious electrons to increase the discharge current dramatically. Short (1 μs), high current (200 A) and high voltage (750 V) discharge pulses are produced in a relaxation instability between the plasma and a charging capacitor. Spots are also observed on a negatively biased plane Langmuir probe. The probe current pulses are as large as those on the magnet, implying that the high discharge current does not depend on the cathode surface area but on the properties of the spots. The fast current pulses produce large inductive voltages, which can reverse the electrical polarity of the magnet and temporarily operate it as an anode. The discharge current may also oscillate at the frequency determined by the charging capacitor and the discharge circuit inductance. Each half cycle of high-current current pulses exhibits a fast (≃10 ns) current rise when a spot is formed. It induces high frequency (10-100 MHz) transients and ringing oscillations in probes and current circuits. Most probes behave like unmatched antennas for the electromagnetic pulses of spot discharges. Examples are shown to distinguish the source of oscillations and some rf characteristics of Langmuir probes.

A beam current density monitor for intense electron beams

NASA Astrophysics Data System (ADS)

Fiorito, R. B.; Raleigh, M.; Seltzer, S. M.

1983-12-01

The authors describe a new type of electric probe for mapping the radial current density profile of high-energy, high current electron beams. The idea of developing an electrically sensitive probe for these conditions was originally suggested to one of the authors during a year's visit to the Lawrence Livermore National Laboratory. The resulting probe is intended for use on the Experimental Test Accelerator (ETA) and the Advanced Test Accelerator at that laboratory. This report discusses in detail: the mechanical design, the electrical response, and temperature effects, as they pertain to the electric probe, and describe the first experimental results obtained using this probe on ETA.

Analysis of multicrystal pump–probe data sets. I. Expressions for the RATIO model

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fournier, Bertrand; Coppens, Philip

2014-08-30

The RATIO method in time-resolved crystallography [Coppenset al.(2009).J. Synchrotron Rad.16, 226–230] was developed for use with Laue pump–probe diffraction data to avoid complex corrections due to wavelength dependence of the intensities. The application of the RATIO method in processing/analysis prior to structure refinement requires an appropriate ratio model for modeling the light response. The assessment of the accuracy of pump–probe time-resolved structure refinements based on the observed ratios was discussed in a previous paper. In the current paper, a detailed ratio model is discussed, taking into account both geometric and thermal light-induced changes.

Studies on the formation and stability of triplex DNA using fluorescence correlation spectroscopy.

PubMed

Hu, Hongyan; Huang, Xiangyi; Ren, Jicun

2016-05-01

Triplex DNA has become one of the most useful recognition motifs in the design of new molecular biology tools, therapeutic agents and sophisticated DNA-based nanomaterials because of its direct recognition of natural double-stranded DNA. In this paper, we developed a sensitive and microscale method to study the formation and stability characterization of triplex DNA using fluorescence correlation spectroscopy (FCS). The principle of this method is mainly based on the excellent capacity of FCS for sensitively distinguishing between free single-strand DNA (ssDNA) fluorescent probes and fluorescent probe-double-strand DNA (dsDNA) hybridized complexes. First, we systematically investigated the experimental conditions of triplex DNA formation. Then, we evaluated the equilibrium association constants (K(a)) under different ssDNA probe lengths, composition and pH. Finally, we used FCS to measure the hybridization fraction of a 20-mer perfectly matched ssDNA probe and three single-base mismatched ssDNA probes with 146-mer dsDNA. Our data illustrated that FCS is a useful tool for the direct determination of the thermodynamic parameters of triplex DNA formation and discrimination of a single-base mismatch of triplex DNA without denaturation. Compared with current methods, our method is characterized by high sensitivity, good universality and small sample and reagent requirements. More importantly, our method has the potential to become a platform for triplex DNA research in vitro. Copyright © 2015 John Wiley & Sons, Ltd.

Validation of Afterbody Aeroheating Predictions for Planetary Probes: Status and Future Work

NASA Technical Reports Server (NTRS)

Wright, Michael J.; Brown, James L.; Sinha, Krishnendu; Candler, Graham V.; Milos, Frank S.; Prabhu, DInesh K.

2005-01-01

A review of the relevant flight conditions and physical models for planetary probe afterbody aeroheating calculations is given. Readily available sources of afterbody flight data and published attempts to computationally simulate those flights are summarized. A current status of the application of turbulence models to afterbody flows is presented. Finally, recommendations for additional analysis and testing that would reduce our uncertainties in our ability to accurately predict base heating levels are given.

A field based detection method for Rose rosette virus using isothermal probe-based Reverse transcription-recombinase polymerase amplification assay.

PubMed

Babu, Binoy; Washburn, Brian K; Ertek, Tülin Sarigül; Miller, Steven H; Riddle, Charles B; Knox, Gary W; Ochoa-Corona, Francisco M; Olson, Jennifer; Katırcıoğlu, Yakup Zekai; Paret, Mathews L

2017-09-01

Rose rosette disease, caused by Rose rosette virus (RRV; genus Emaravirus) is a major threat to the rose industry in the U.S. The only strategy currently available for disease management is early detection and eradication of the infected plants, thereby limiting its potential spread. Current RT-PCR based diagnostic methods for RRV are time consuming and are inconsistent in detecting the virus from symptomatic plants. Real-time RT-qPCR assay is highly sensitive for detection of RRV, but it is expensive and requires well-equipped laboratories. Both the RT-PCR and RT-qPCR cannot be used in a field-based testing for RRV. Hence a novel probe based, isothermal reverse transcription-recombinase polymerase amplification (RT-exoRPA) assay, using primer/probe designed based on the nucleocapsid gene of the RRV has been developed. The assay is highly specific and did not give a positive reaction to other viruses infecting roses belonging to both inclusive and exclusive genus. Dilution assays using the in vitro transcript showed that the primer/probe set is highly sensitive, with a detection limit of 1 fg/μl. In addition, a rapid technique for the extraction of viral RNA (<5min) has been standardized from RRV infected tissue sources, using PBS-T buffer (pH 7.4), which facilitates the virus adsorption onto the PCR tubes at 4°C for 2min, followed by denaturation to release the RNA. RT-exoRPA analysis of the infected plants using the primer/probe indicated that the virus could be detected from leaves, stems, petals, pollen, primary roots and secondary roots. In addition, the assay was efficiently used in the diagnosis of RRV from different rose varieties, collected from different states in the U.S. The entire process, including the extraction can be completed in 25min, with less sophisticated equipments. The developed assay can be used with high efficiency in large scale field testing for rapid detection of RRV in commercial nurseries and landscapes. Copyright © 2017 Elsevier B.V. All rights reserved.

An integrated eddy current detection and imaging system on a silicon chip

NASA Technical Reports Server (NTRS)

Henderson, H. Thurman; Kartalia, K. P.; Dury, Joseph D.

1991-01-01

Eddy current probes have been used for many years for numerous sensing applications including crack detection in metals. However, these applications have traditionally used the eddy current effect in the form of a physically wound single or different probe pairs which of necessity must be made quite large compared to microelectronics dimensions. Also, the traditional wound probe can only take a point reading, although that point might include tens of individual cracks or crack arrays; thus, conventional eddy current probes are beset by two major problems: (1) no detailed information can be obtained about the crack or crack array; and (2) for applications such as quality assurance, a vast amount of time must be taken to scan a complete surface. Laboratory efforts have been made to fabricate linear arrays of single turn probes in a thick film format on a ceramic substrate as well as in a flexible cable format; however, such efforts inherently suffer from relatively large size requirements as well as sensitivity issues. Preliminary efforts to fully extend eddy current probing from a point or single dimensional level to a two dimensional micro-eddy current format on a silicon chip, which might overcome all of the above problems, are presented.

A hemispherical Langmuir probe array detector for angular resolved measurements on droplet-based laser-produced plasmas

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gambino, Nadia, E-mail: gambinon@ethz.ch; Brandstätter, Markus; Rollinger, Bob

2014-09-15

In this work, a new diagnostic tool for laser-produced plasmas (LPPs) is presented. The detector is based on a multiple array of six motorized Langmuir probes. It allows to measure the dynamics of a LPP in terms of charged particles detection with particular attention to droplet-based LPP sources for EUV lithography. The system design permits to temporally resolve the angular and radial plasma charge distribution and to obtain a hemispherical mapping of the ions and electrons around the droplet plasma. The understanding of these dynamics is fundamental to improve the debris mitigation techniques for droplet-based LPP sources. The device hasmore » been developed, built, and employed at the Laboratory for Energy Conversion, ETH Zürich. The experimental results have been obtained on the droplet-based LPP source ALPS II. For the first time, 2D mappings of the ion kinetic energy distribution around the droplet plasma have been obtained with an array of multiple Langmuir probes. These measurements show an anisotropic expansion of the ions in terms of kinetic energy and amount of ion charge around the droplet target. First estimations of the plasma density and electron temperature were also obtained from the analysis of the probe current signals.« less

Graphing Misconceptions and Possible Remedies Using Microcomputer-Based Labs.

ERIC Educational Resources Information Center

Barclay, William L.

Graphing is a common and powerful symbol system for representing concrete data. Yet research has shown that students often have graphical misconceptions about how graphs are related to the concrete event. Currently, the Technical Education Research Center (TERC) is developing microcomputer-based laboratories (MBL) science units that use probes to…

Effects of computer-based training on procedural modifications to standard functional analyses.

PubMed

Schnell, Lauren K; Sidener, Tina M; DeBar, Ruth M; Vladescu, Jason C; Kahng, SungWoo

2018-01-01

Few studies have evaluated methods for training decision-making when functional analysis data are undifferentiated. The current study evaluated computer-based training to teach 20 graduate students to arrange functional analysis conditions, analyze functional analysis data, and implement procedural modifications. Participants were exposed to training materials using interactive software during a 1-day session. Following the training, mean scores on the posttest, novel cases probe, and maintenance probe increased for all participants. These results replicate previous findings during a 1-day session and include a measure of participant acceptability of the training. Recommendations for future research on computer-based training and functional analysis are discussed. © 2017 Society for the Experimental Analysis of Behavior.

Mass Spectrometry for Paper-Based Immunoassays: Toward On-Demand Diagnosis.

PubMed

Chen, Suming; Wan, Qiongqiong; Badu-Tawiah, Abraham K

2016-05-25

Current analytical methods, either point-of-care or centralized detection, are not able to meet recent demands of patient-friendly testing and increased reliability of results. Here, we describe a two-point separation on-demand diagnostic strategy based on a paper-based mass spectrometry immunoassay platform that adopts stable and cleavable ionic probes as mass reporter; these probes make possible sensitive, interruptible, storable, and restorable on-demand detection. In addition, a new touch paper spray method was developed for on-chip, sensitive, and cost-effective analyte detection. This concept is successfully demonstrated via (i) the detection of Plasmodium falciparum histidine-rich protein 2 antigen and (ii) multiplexed and simultaneous detection of cancer antigen 125 and carcinoembryonic antigen.

Bohm-criterion approximation versus optimal matched solution for a cylindrical probe in radial-motion theory

DOE Office of Scientific and Technical Information (OSTI.GOV)

Din, Alif

2016-08-15

The theory of positive-ion collection by a probe immersed in a low-pressure plasma was reviewed and extended by Allen et al. [Proc. Phys. Soc. 70, 297 (1957)]. The numerical computations for cylindrical and spherical probes in a sheath region were presented by F. F. Chen [J. Nucl. Energy C 7, 41 (1965)]. Here, in this paper, the sheath and presheath solutions for a cylindrical probe are matched through a numerical matching procedure to yield “matched” potential profile or “M solution.” The solution based on the Bohm criterion approach “B solution” is discussed for this particular problem. The comparison of cylindricalmore » probe characteristics obtained from the correct potential profile (M solution) and the approximated Bohm-criterion approach are different. This raises questions about the correctness of cylindrical probe theories relying only on the Bohm-criterion approach. Also the comparison between theoretical and experimental ion current characteristics shows that in an argon plasma the ions motion towards the probe is almost radial.« less

Optimization of a reusable, DNA pseudoknot-based electrochemical sensor for sequence-specific DNA detection in blood serum.

PubMed

Cash, Kevin J; Heeger, Alan J; Plaxco, Kevin W; Xiao, Yi

2009-01-15

We describe in detail a new electrochemical DNA (E-DNA) sensing platform based on target-induced conformation changes in an electrode-bound DNA pseudoknot. The pseudoknot, a DNA structure containing two stem-loops in which the first stem's loop forms part of the second stem, is modified with a methylene blue redox tag at its 3' terminus and covalently attached to a gold electrode via the 5' terminus. In the absence of a target, the structure of the pseudoknot probe minimizes collisions between the redox tag and the electrode, thus reducing faradaic current. Target binding disrupts the pseudoknot structure, liberating a flexible, single-stranded element that can strike the electrode and efficiently transfer electrons. In this article we report further characterization and optimization of this new E-DNA architecture. We find that optimal signaling is obtained at an intermediate probe density ( approximately 1.8 x 10(13) molecules/cm(2) apparent density), which presumably represents a balance between steric and electrostatic blocking at high probe densities and increased background currents arising from transfer from the pseudoknot probe at lower densities. We also find that optimal 3' stem length, which appears to be 7 base pairs, represents a balance between pseudoknot structural stability and target affinity. Finally, a 3' loop comprised of poly(A) exhibits better mismatch discrimination than the equivalent poly(T) loop, but at the cost of decreased gain. Optimization over this parameter space significantly improves the signaling of the pseudoknot-based E-DNA architecture, leading to the ability to sensitively and specifically detect DNA targets even when challenged in complex, multicomponent samples such as blood serum.

Optimization of a Reusable, DNA Pseudoknot-Based Electrochemical Sensor for Sequence-Specific DNA Detection in Blood Serum

PubMed Central

Cash, Kevin J.; Heeger, Alan J.; Plaxco, Kevin W.; Xiao, Yi

2010-01-01

We describe in detail a new electrochemical DNA (E-DNA) sensing platform based on target-induced conformation changes in an electrode-bound DNA pseudoknot. The pseudoknot, a DNA structure containing two stem-loops in which the first stem’s loop forms part of the second stem, is modified with a methylene blue redox tag at its 3′ terminus and covalently attached to a gold electrode via the 5′ terminus. In the absence of a target, the structure of the pseudoknot probe minimizes collisions between the redox tag and the electrode, thus reducing faradaic current. Target binding disrupts the pseudoknot structure, liberating a flexible, single-stranded element that can strike the electrode and efficiently transfer electrons. In this article we report further characterization and optimization of this new E-DNA architecture. We find that optimal signaling is obtained at an intermediate probe density (~1.8 × 1013 molecules/cm2 apparent density), which presumably represents a balance between steric and electrostatic blocking at high probe densities and increased background currents arising from transfer from the pseudoknot probe at lower densities. We also find that optimal 3′ stem length, which appears to be 7 base pairs, represents a balance between pseudoknot structural stability and target affinity. Finally, a 3′ loop comprised of poly(A) exhibits better mismatch discrimination than the equivalent poly(T) loop, but at the cost of decreased gain. Optimization over this parameter space significantly improves the signaling of the pseudoknot-based E-DNA architecture, leading to the ability to sensitively and specifically detect DNA targets even when challenged in complex, multicomponent samples such as blood serum. PMID:19093760

The Goal Structure of a Socratic Tutor. Technical Report No. 3.

ERIC Educational Resources Information Center

Stevens, Albert L.; Collins, Allan

This report describes the current version of the Why System, a script-based Socratic tutor which uses tutoring strategies formulated as production rules. The current system is capable of carrying on a dialogue about the factors influencing rainfall by presenting different cases to the student, asking for predictions, probing for relevant factors,…

A new evaluation method of electron optical performance of high beam current probe forming systems.

PubMed

Fujita, Shin; Shimoyama, Hiroshi

2005-10-01

A new numerical simulation method is presented for the electron optical property analysis of probe forming systems with point cathode guns such as cold field emitters and the Schottky emitters. It has long been recognized that the gun aberrations are important parameters to be considered since the intrinsically high brightness of the point cathode gun is reduced due to its spherical aberration. The simulation method can evaluate the 'threshold beam current I(th)' above which the apparent brightness starts to decrease from the intrinsic value. It is found that the threshold depends on the 'electron gun focal length' as well as on the spherical aberration of the gun. Formulas are presented to estimate the brightness reduction as a function of the beam current. The gun brightness reduction must be included when the probe property (the relation between the beam current l(b) and the probe size on the sample, d) of the entire electron optical column is evaluated. Formulas that explicitly consider the gun aberrations into account are presented. It is shown that the probe property curve consists of three segments in the order of increasing beam current: (i) the constant probe size region, (ii) the brightness limited region where the probe size increases as d approximately I(b)(3/8), and (iii) the angular current intensity limited region in which the beam size increases rapidly as d approximately I(b)(3/2). Some strategies are suggested to increase the threshold beam current and to extend the effective beam current range of the point cathode gun into micro ampere regime.

Effects of Angular Variation on Split D Differential Eddy Current Probe Response (Postprint)

DTIC Science & Technology

2016-02-10

AFRL-RX-WP-JA-2016-0327 EFFECTS OF ANGULAR VARIATION ON SPLIT D DIFFERENTIAL EDDY CURRENT PROBE RESPONSE (POSTPRINT) Ryan D...March 2014 – 22 September 2015 4. TITLE AND SUBTITLE EFFECTS OF ANGULAR VARIATION ON SPLIT D DIFFERENTIAL EDDY CURRENT PROBE RESPONSE (POSTPRINT...last few years have seen increased levels of complexity added to push the state-of-the-art modeling software used in eddy current NDE today. The added

Electrical impedance map (EIM) for margin assessment during robot-assisted laparoscopic prostatectomy (RALP) using a microendoscopic probe

NASA Astrophysics Data System (ADS)

Mahara, Aditya; Khan, Shadab; Schned, Alan R.; Hyams, Elias S.; Halter, Ryan J.

2015-03-01

Positive surgical margins (PSMs) found following prostate cancer surgery are a significant risk factor for post-operative disease recurrence. Noxious adjuvant radiation and chemical-based therapies are typically offered to men with PSMs. Unfortunately, no real-time intraoperative technology is currently available to guide surgeons to regions of suspicion during the initial prostatectomy where immediate surgical excisions could be used to reduce the chance of PSMs. A microendoscopic electrical impedance sensing probe was developed with the intention of providing real-time feedback regarding margin status to surgeons during robot-assisted laparoscopic prostatectomy (RALP) procedures. A radially configured 17-electrode microendoscopic probe was designed, constructed, and initially evaluated through use of gelatin-based phantoms and an ex vivo human prostate specimen. Impedance measurements are recorded at 10 frequencies (10 kHz - 100 kHz) using a high-speed FPGA-based electrical impedance tomography (EIT) system. Tetrapolar impedances are recorded from a number of different electrode configurations strategically chosen to sense tissue in a pre-defined sector underlying the probe face. A circular electrical impedance map (EIM) with several color-coded pie-shaped sectors is created to represent the impedance values of the probed tissue. Gelatin phantom experiments show an obvious distinction in the impedance maps between high and low impedance regions. Similarly, the EIM generated from the ex vivo prostate case shows distinguishing features between cancerous and benign regions. Based on successful development of this probe and these promising initial results, EIMs of additional prostate specimens are being collected to further evaluate this approach for intraoperative surgical margin assessment during RALP procedures.

Electron launching voltage monitor

DOEpatents

Mendel, Clifford W.; Savage, Mark E.

1992-01-01

An electron launching voltage monitor measures MITL voltage using a relationship between anode electric field and electron current launched from a cathode-mounted perturbation. An electron launching probe extends through and is spaced from the edge of an opening in a first MITL conductor, one end of the launching probe being in the gap between the MITL conductor, the other end being adjacent a first side of the first conductor away from the second conductor. A housing surrounds the launching probe and electrically connects the first side of the first conductor to the other end of the launching probe. A detector detects the current passing through the housing to the launching probe, the detected current being representative of the voltage between the conductors.

Amplitude-Stabilized Oscillator for a Capacitance-Probe Electrometer

NASA Technical Reports Server (NTRS)

Blaes, Brent R.; Schaefer, Rembrandt T.

2012-01-01

A multichannel electrometer voltmeter that employs a mechanical resonator maintained in sustained amplitude-stabilized oscillation has been developed for the space-based measurement of an Internal Electrostatic Discharge Monitor (IESDM) sensor. The IESDM is new sensor technology targeted for integration into a Space Environmental Monitor (SEM) subsystem used for the characterization and monitoring of deep dielectric charging on spacecraft. Creating a stable oscillator from the mechanical resonator was achieved by employing magnetic induction for sensing the resonator s velocity, and forcing a current through a coil embedded in the resonator to produce a Lorentz actuation force that overcomes the resonator s dissipative losses. Control electronics employing an AGC loop provide conditions for stabilized, constant amplitude harmonic oscillation. The prototype resonator was composed of insulating FR4 printed-wireboard (PWB) material containing a flat, embedded, rectangular coil connected through flexure springs to a base PWB, and immersed in a magnetic field having two regions of opposite field direction generated by four neodymium block magnets. In addition to maintaining the mechanical movement needed for the electrometer s capacitor-probe transducer, this oscillator provides a reference signal for synchronous detection of the capacitor probe s output signal current so drift of oscillation frequency due to environmental effects is inconsequential.

Global Environmental Micro Sensors Test Operations in the Natural Environment

NASA Technical Reports Server (NTRS)

Adams, Mark L.; Buza, Matthew; Manobianco, John; Merceret, Francis J.

2007-01-01

ENSCO, Inc. is developing an innovative atmospheric observing system known as Global Environmental Micro Sensors (GEMS). The GEMS concept features an integrated system of miniaturized in situ, airborne probes measuring temperature, relative humidity, pressure, and vector wind velocity. In order for the probes to remain airborne for long periods of time, their design is based on a helium-filled super-pressure balloon. The GEMS probes are neutrally buoyant and carried passively by the wind at predetermined levels. Each probe contains onboard satellite communication, power generation, processing, and geolocation capabilities. ENSCO has partnered with the National Aeronautics and Space Administration's Kennedy Space Center (KSC) for a project called GEMS Test Operations in the Natural Environment (GEMSTONE) that will culminate with limited prototype flights of the system in spring 2007. By leveraging current advances in micro and nanotechnology, the probe mass, size, cost, and complexity can be reduced substantially so that large numbers of probes could be deployed routinely to support ground, launch, and landing operations at KSC and other locations. A full-scale system will improve the data density for the local initialization of high-resolution numerical weather prediction systems by at least an order of magnitude and provide a significantly expanded in situ data base to evaluate launch commit criteria and flight rules. When applied to launch or landing sites, this capability will reduce both weather hazards and weather-related scrubs, thus enhancing both safety and cost-avoidance for vehicles processed by the Shuttle, Launch Services Program, and Constellation Directorates. The GEMSTONE project will conclude with a field experiment in which 10 to 15 probes are released over KSC in east central Florida. The probes will be neutrally buoyant at different altitudes from 500 to 3000 meters and will report their position, speed, heading, temperature, humidity, and pressure via satellite. The GEMS data will be validated against reference observations provided by current weather instrumentation located at KSC. This paper will report on the results of the GEMSTONE project and discuss the challenges encountered in developing an airborne sensor system.

Optical imaging probes in oncology

PubMed Central

Martelli, Cristina; Dico, Alessia Lo; Diceglie, Cecilia; Lucignani, Giovanni; Ottobrini, Luisa

2016-01-01

Cancer is a complex disease, characterized by alteration of different physiological molecular processes and cellular features. Keeping this in mind, the possibility of early identification and detection of specific tumor biomarkers by non-invasive approaches could improve early diagnosis and patient management. Different molecular imaging procedures provide powerful tools for detection and non-invasive characterization of oncological lesions. Clinical studies are mainly based on the use of computed tomography, nuclear-based imaging techniques and magnetic resonance imaging. Preclinical imaging in small animal models entails the use of dedicated instruments, and beyond the already cited imaging techniques, it includes also optical imaging studies. Optical imaging strategies are based on the use of luminescent or fluorescent reporter genes or injectable fluorescent or luminescent probes that provide the possibility to study tumor features even by means of fluorescence and luminescence imaging. Currently, most of these probes are used only in animal models, but the possibility of applying some of them also in the clinics is under evaluation. The importance of tumor imaging, the ease of use of optical imaging instruments, the commercial availability of a wide range of probes as well as the continuous description of newly developed probes, demonstrate the significance of these applications. The aim of this review is providing a complete description of the possible optical imaging procedures available for the non-invasive assessment of tumor features in oncological murine models. In particular, the characteristics of both commercially available and newly developed probes will be outlined and discussed. PMID:27145373

High speed displacement measurement based on electro-magnetic induction applied to electromagnetically driven ring expansion

NASA Astrophysics Data System (ADS)

Han, Xiaotao; Wu, Jiawei; Huang, Lantao; Qiu, Lei; Chen, Qi; Cao, Quanliang; Herlach, Fritz; Li, Liang

2017-11-01

Investigating the mechanism of electromagnetic forming (EMF) becomes a hot topic in the field of metal forming. The high speed up to 200 m/s in EMF makes it a real challenge to capture the forming process. To this end, a new method for measuring displacement at high speed based on electromagnetic induction has been developed. Specifically this is used to measure the displacement of an expanding metal ring driven by a pulsed magnetic field; this is one of the basic EMF processes. The new method is simple and practical, and it combines high-speed response with adequate precision. The new measurement system consists of a printed circuit board (PCB) and a Rogowski probe. Eleven coaxial annular detecting probes are arranged in the PCB plate to acquire induced voltage at different positions, and a Rogowski probe is used to measure the current in the driving coil. The displacement of the ring is deduced by analyzing the output voltages of the detecting probes and the Rogowski probe. The feasibility of the method is verified by comparing the results with pictures from a high speed camera taken simultaneously.

Study on electrostatic and electromagnetic probes operated in ceramic and metallic depositing plasmas

NASA Astrophysics Data System (ADS)

Styrnoll, T.; Bienholz, S.; Lapke, M.; Awakowicz, P.

2014-04-01

This paper discusses plasma probe diagnostics, namely the multipole resonance probe (MRP) and Langmuir probe (LP), operated in depositing plasmas. The aim of this work is to show that the combination of both probes provides stable and robust measurements and clear determination of plasma parameters for metallic and ceramic coating processes. The probes use different approaches to determine plasma parameters, e.g. electron density ne and electron temperature Te. The LP is a well-established plasma diagnostic, and its applicability in technological plasmas is well documented. The LP is a dc probe that performs a voltage sweep and analyses the measured current, which makes it insensitive against conductive metallic coating. However, once the LP is dielectrically coated with a ceramic film, its functionality is constricted. In contrast, the MRP was recently presented as a monitoring tool, which is insensitive to coating with dielectric ceramics. It is a new plasma diagnostic based on the concept of active plasma resonance spectroscopy, which uses the universal characteristic of all plasmas to resonate on or near the electron plasma frequency. The MRP emits a frequency sweep and the absorption of the signal, the |S11| parameter, is analysed. Since the MRP concept is based on electromagnetic waves, which are able to transmit dielectrics, it is insensitive to dielectric coatings. But once the MRP is metallized with a thin conductive film, no undisturbed RF-signal can be emitted into the plasma, which leads to falsified plasma parameter. In order to compare both systems, during metallic or dielectric coating, the probes are operated in a magnetron CCP, which is equipped with a titanium target. We present measurements in metallic and dielectric coating processes with both probes and elaborate advantages and problems of each probe operated in each coating environment.

A new B-dot probe-based diagnostic for amplitude, polarization, and wavenumber measurements of ion cyclotron range-of frequency fields on ASDEX Upgrade

NASA Astrophysics Data System (ADS)

Ochoukov, R.; Bobkov, V.; Faugel, H.; Fünfgelder, H.; Noterdaeme, J.-M.

2015-11-01

A new B-dot probe-based diagnostic has been installed on an ASDEX Upgrade tokamak to characterize ion cyclotron range-of frequency (ICRF) wave generation and interaction with magnetized plasma. The diagnostic consists of a field-aligned array of B-dot probes, oriented to measure fast and slow ICRF wave fields and their field-aligned wavenumber (k//) spectrum on the low field side of ASDEX Upgrade. A thorough description of the diagnostic and the supporting electronics is provided. In order to compare the measured dominant wavenumber of the local ICRF fields with the expected spectrum of the launched ICRF waves, in-air near-field measurements were performed on the newly installed 3-strap ICRF antenna to reconstruct the dominant launched toroidal wavenumbers (ktor). Measurements during a strap current phasing scan in tokamak discharges reveal an upshift in k// as strap phasing is moved away from the dipole configuration. This result is the opposite of the ktor trend expected from in-air near-field measurements; however, the near-field based reconstruction routine does not account for the effect of induced radiofrequency (RF) currents in the passive antenna structures. The measured exponential increase in the local ICRF wave field amplitude is in agreement with the upshifted k//, as strap phasing moves away from the dipole configuration. An examination of discharges heated with two ICRF antennas simultaneously reveals the existence of beat waves at 1 kHz, as expected from the difference of the two antennas' operating frequencies. Beats are observed on both the fast and the slow wave probes suggesting that the two waves are coupled outside the active antennas. Although the new diagnostic shows consistent trends between the amplitude and the phase measurements in response to changes applied by the ICRF antennas, the disagreement with the in-air near-field measurements remains. An electromagnetic model is currently under development to address this issue.

A new B-dot probe-based diagnostic for amplitude, polarization, and wavenumber measurements of ion cyclotron range-of frequency fields on ASDEX Upgrade.

PubMed

Ochoukov, R; Bobkov, V; Faugel, H; Fünfgelder, H; Noterdaeme, J-M

2015-11-01

A new B-dot probe-based diagnostic has been installed on an ASDEX Upgrade tokamak to characterize ion cyclotron range-of frequency (ICRF) wave generation and interaction with magnetized plasma. The diagnostic consists of a field-aligned array of B-dot probes, oriented to measure fast and slow ICRF wave fields and their field-aligned wavenumber (k(//)) spectrum on the low field side of ASDEX Upgrade. A thorough description of the diagnostic and the supporting electronics is provided. In order to compare the measured dominant wavenumber of the local ICRF fields with the expected spectrum of the launched ICRF waves, in-air near-field measurements were performed on the newly installed 3-strap ICRF antenna to reconstruct the dominant launched toroidal wavenumbers (k(tor)). Measurements during a strap current phasing scan in tokamak discharges reveal an upshift in k(//) as strap phasing is moved away from the dipole configuration. This result is the opposite of the k(tor) trend expected from in-air near-field measurements; however, the near-field based reconstruction routine does not account for the effect of induced radiofrequency (RF) currents in the passive antenna structures. The measured exponential increase in the local ICRF wave field amplitude is in agreement with the upshifted k(//), as strap phasing moves away from the dipole configuration. An examination of discharges heated with two ICRF antennas simultaneously reveals the existence of beat waves at 1 kHz, as expected from the difference of the two antennas' operating frequencies. Beats are observed on both the fast and the slow wave probes suggesting that the two waves are coupled outside the active antennas. Although the new diagnostic shows consistent trends between the amplitude and the phase measurements in response to changes applied by the ICRF antennas, the disagreement with the in-air near-field measurements remains. An electromagnetic model is currently under development to address this issue.

Eddy current probe response to open and closed surface flaws

NASA Technical Reports Server (NTRS)

Auld, B. A.; Muennemann, F.; Winslow, D. K.

1981-01-01

A general analysis of eddy current response to certain types of open and closed surface flaws is presented for both standard low-frequency and ferromagnetic-resonance (FMR) probes. It is shown analytically that for two-dimensional and three-dimensional surface flaws interrogated by a uniform probe field, the crack opening sensitivity increases with the operating frequency of the probe, this behavior being due to the Faraday induction effect. Experiments with low-frequency probes operating at or below 1 MHz and with the FMR probe operating at approximately 1000 MHz confirm this increase of the crack mouth opening displacement for practical situations where the probe field is not uniform in the vicinity of the flaw.

Iterative Bayesian Estimation of Travel Times on Urban Arterials: Fusing Loop Detector and Probe Vehicle Data.

PubMed

Liu, Kai; Cui, Meng-Ying; Cao, Peng; Wang, Jiang-Bo

2016-01-01

On urban arterials, travel time estimation is challenging especially from various data sources. Typically, fusing loop detector data and probe vehicle data to estimate travel time is a troublesome issue while considering the data issue of uncertain, imprecise and even conflicting. In this paper, we propose an improved data fusing methodology for link travel time estimation. Link travel times are simultaneously pre-estimated using loop detector data and probe vehicle data, based on which Bayesian fusion is then applied to fuse the estimated travel times. Next, Iterative Bayesian estimation is proposed to improve Bayesian fusion by incorporating two strategies: 1) substitution strategy which replaces the lower accurate travel time estimation from one sensor with the current fused travel time; and 2) specially-designed conditions for convergence which restrict the estimated travel time in a reasonable range. The estimation results show that, the proposed method outperforms probe vehicle data based method, loop detector based method and single Bayesian fusion, and the mean absolute percentage error is reduced to 4.8%. Additionally, iterative Bayesian estimation performs better for lighter traffic flows when the variability of travel time is practically higher than other periods.

Iterative Bayesian Estimation of Travel Times on Urban Arterials: Fusing Loop Detector and Probe Vehicle Data

PubMed Central

Cui, Meng-Ying; Cao, Peng; Wang, Jiang-Bo

2016-01-01

On urban arterials, travel time estimation is challenging especially from various data sources. Typically, fusing loop detector data and probe vehicle data to estimate travel time is a troublesome issue while considering the data issue of uncertain, imprecise and even conflicting. In this paper, we propose an improved data fusing methodology for link travel time estimation. Link travel times are simultaneously pre-estimated using loop detector data and probe vehicle data, based on which Bayesian fusion is then applied to fuse the estimated travel times. Next, Iterative Bayesian estimation is proposed to improve Bayesian fusion by incorporating two strategies: 1) substitution strategy which replaces the lower accurate travel time estimation from one sensor with the current fused travel time; and 2) specially-designed conditions for convergence which restrict the estimated travel time in a reasonable range. The estimation results show that, the proposed method outperforms probe vehicle data based method, loop detector based method and single Bayesian fusion, and the mean absolute percentage error is reduced to 4.8%. Additionally, iterative Bayesian estimation performs better for lighter traffic flows when the variability of travel time is practically higher than other periods. PMID:27362654

Corrosion Detection in Airframes Using a New Flux-Focusing Eddy Current Probe

NASA Technical Reports Server (NTRS)

Fulton, James P.; Wincheski, Buzz; Nath, Shridhar; Namkung, Min

1994-01-01

A new flux-focusing eddy current probe was recently developed at NASA Langley Research Center. The new probe is similar in design to a reflection type eddy current probe, but is unique in that it does not require the use of an impedance bridge for balancing. The device monitors the RMS output voltage of a pickup coil and, as a result, is easier to operate and interpret than traditional eddy current instruments. The unique design feature of the probe is a ferromagnetic cylinder, typically 1020 steel, which separates a concentrically positioned drive and pickup coil. The increased permeability of the steel causes the magnetic flux produced by the drive coil to be focused in a ring around the pickup coil. At high frequencies the eddy currents induced in both the sample and the cylinder allow little or no flux to link with the pickup coil. This results in a self-nulling condition which has been shown to be useful for the unambiguous detection of cracks in conducting materials. As the frequency is lowered the flux produced by the drive coil begins to link with the pickup coil causing an output which, among other things, is proportional to the thickness of the test specimen. This enables highly accurate measurements of the thickness of conducting materials and helps to facilitate the monitoring of thickness variations in a conducting structure such as an aircraft fuselage. Under ideal laboratory conditions the probe can sense thickness changes on the order of 1% as illustrated. However, this is highly dependent upon the thickness, and the geometric complexity of the sample being tested and for practical problems the sensitivity is usually much less. In this presentation we highlight some of the advantages and limitations in using the probe to inspect aircraft panels for corrosion and other types of material nonuniformities. In particular, we present preliminary results which illustrate the probes capabilities for detecting first and second layer corrosion in aircraft panels which may contain air gaps between the layers. Since the probe utilized eddy currents its corrosion detection capabilities are similar to convectional eddy current techniques, but the new probe is much easier to use.

Pump-Probe Noise Spectroscopy of Molecular Junctions.

PubMed

Ochoa, Maicol A; Selzer, Yoram; Peskin, Uri; Galperin, Michael

2015-02-05

The slow response of electronic components in junctions limits the direct applicability of pump-probe type spectroscopy in assessing the intramolecular dynamics. Recently the possibility of getting information on a sub-picosecond time scale from dc current measurements was proposed. We revisit the idea of picosecond resolution by pump-probe spectroscopy from dc measurements and show that any intramolecular dynamics not directly related to charge transfer in the current direction is missed by current measurements. We propose a pump-probe dc shot noise spectroscopy as a suitable alternative. Numerical examples of time-dependent and average responses of junctions are presented for generic models.

Electron launching voltage monitor

DOEpatents

Mendel, C.W.; Savage, M.E.

1992-03-17

An electron launching voltage monitor measures MITL voltage using a relationship between anode electric field and electron current launched from a cathode-mounted perturbation. An electron launching probe extends through and is spaced from the edge of an opening in a first MITL conductor, one end of the launching probe being in the gap between the MITL conductor, the other end being adjacent a first side of the first conductor away from the second conductor. A housing surrounds the launching probe and electrically connects the first side of the first conductor to the other end of the launching probe. A detector detects the current passing through the housing to the launching probe, the detected current being representative of the voltage between the conductors. 5 figs.

A rational regulatory approach for positron emission tomography imaging probes: from "first in man" to NDA approval and reimbursement.

PubMed

Barrio, Jorge R; Marcus, Carol S; Hung, Joseph C; Keppler, Jennifer S

2004-01-01

We propose a new regulatory approach for positron emission tomography (PET) molecular imaging probes, essential tools in today's medicine. Even though the focus of this paper is on positron-emitting labeled probes, it is also justified to extend this proposed regulatory approach to other diagnostic nuclear medicine radiopharmaceuticals. Key aspects of this proposal include: (1) PET molecular imaging probes would be placed in a "no significant risk" category, similar to that category for devices in current Food and Drug Administration (FDA) regulations, based on overwhelming scientific evidence that demonstrates their faultless safety profile; (2) the FDA-sanctioned Radioactive Drug Research Committee (RDRC) will oversee all diagnostic research with these probes. The newly defined RDRC should approve "first in man" use; supervise a broader spectrum of diagnostic research protocols, including those looking to demonstrate initial efficacy, as well as multicenter clinical trials and the use of molecular imaging probes as a screening tool in drug discovery. The current investigational new drug (IND) mechanism is thus eliminated for these diagnostic probes; (3) when a molecular imaging probe has demonstrated diagnostic efficacy, FDA approval (i.e., NDA) will be sought. The review will be done by a newly constituted Radioactive Drug Advisory Committee (RDAC) composed of experts chosen by the professional societies, who would provide a binding assessment of the adequacy of the safety and efficacy data. When the RDAC recommends its diagnostic use on scientific and medical grounds, the molecular imaging probe becomes FDA approved. After a molecular imaging probe is approved for a diagnostic indication, the existing mechanism to seek reimbursement will be utilized; and (4) the FDA would retain its direct oversight function for traditional manufacturers engaged in commercial distribution of the approved diagnostic molecular imaging probes (i.e., under NDA) to monitor compliance with existing US Pharmacopeia (USP) requirements. With abbreviated and more appropriate regulations, new PET molecular imaging probes for diagnostic use would be then rapidly incorporated into the mainstream diagnostic medicine. Equally importantly, this approach would facilitate the use of molecular imaging in drug discovery and development, which would substantially reduce the costs and time required to bring new therapeutic drugs to market.

Bringing Standardized Processes in Atom-Probe Tomography: I Establishing Standardized Terminology

DOE Office of Scientific and Technical Information (OSTI.GOV)

Anderson, Ian M; Danoix, F; Forbes, Richard

2011-01-01

Defining standardized methods requires careful consideration of the entire field and its applications. The International Field Emission Society (IFES) has elected a Standards Committee, whose task is to determine the needed steps to establish atom-probe tomography as an accepted metrology technique. Specific tasks include developing protocols or standards for: terminology and nomenclature; metrology and instrumentation, including specifications for reference materials; test methodologies; modeling and simulations; and science-based health, safety, and environmental practices. The Committee is currently working on defining terminology related to atom-probe tomography with the goal to include terms into a document published by the International Organization for Standardsmore » (ISO). A lot of terms also used in other disciplines have already been defined) and will be discussed for adoption in the context of atom-probe tomography.« less

In-flight calibration of mesospheric rocket plasma probes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Havnes, Ove; University Studies Svalbard; Hartquist, Thomas W.

Many effects and factors can influence the efficiency of a rocket plasma probe. These include payload charging, solar illumination, rocket payload orientation and rotation, and dust impact induced secondary charge production. As a consequence, considerable uncertainties can arise in the determination of the effective cross sections of plasma probes and measured electron and ion densities. We present a new method for calibrating mesospheric rocket plasma probes and obtaining reliable measurements of plasma densities. This method can be used if a payload also carries a probe for measuring the dust charge density. It is based on that a dust probe's effectivemore » cross section for measuring the charged component of dust normally is nearly equal to its geometric cross section, and it involves the comparison of variations in the dust charge density measured with the dust detector to the corresponding current variations measured with the electron and/or ion probes. In cases in which the dust charge density is significantly smaller than the electron density, the relation between plasma and dust charge density variations can be simplified and used to infer the effective cross sections of the plasma probes. We illustrate the utility of the method by analysing the data from a specific rocket flight of a payload containing both dust and electron probes.« less

In-flight calibration of mesospheric rocket plasma probes.

PubMed

Havnes, Ove; Hartquist, Thomas W; Kassa, Meseret; Morfill, Gregor E

2011-07-01

Many effects and factors can influence the efficiency of a rocket plasma probe. These include payload charging, solar illumination, rocket payload orientation and rotation, and dust impact induced secondary charge production. As a consequence, considerable uncertainties can arise in the determination of the effective cross sections of plasma probes and measured electron and ion densities. We present a new method for calibrating mesospheric rocket plasma probes and obtaining reliable measurements of plasma densities. This method can be used if a payload also carries a probe for measuring the dust charge density. It is based on that a dust probe's effective cross section for measuring the charged component of dust normally is nearly equal to its geometric cross section, and it involves the comparison of variations in the dust charge density measured with the dust detector to the corresponding current variations measured with the electron and/or ion probes. In cases in which the dust charge density is significantly smaller than the electron density, the relation between plasma and dust charge density variations can be simplified and used to infer the effective cross sections of the plasma probes. We illustrate the utility of the method by analysing the data from a specific rocket flight of a payload containing both dust and electron probes.

PCR-free quantitative detection of genetically modified organism from raw materials – A novel electrochemiluminescence-based bio-barcode method

PubMed Central

Zhu, Debin; Tang, Yabing; Xing, Da; Chen, Wei R.

2018-01-01

Bio-barcode assay based on oligonucleotide-modified gold nanoparticles (Au-NPs) provides a PCR-free method for quantitative detection of nucleic acid targets. However, the current bio-barcode assay requires lengthy experimental procedures including the preparation and release of barcode DNA probes from the target-nanoparticle complex, and immobilization and hybridization of the probes for quantification. Herein, we report a novel PCR-free electrochemiluminescence (ECL)-based bio-barcode assay for the quantitative detection of genetically modified organism (GMO) from raw materials. It consists of tris-(2’2’-bipyridyl) ruthenium (TBR)-labele barcode DNA, nucleic acid hybridization using Au-NPs and biotin-labeled probes, and selective capture of the hybridization complex by streptavidin-coated paramagnetic beads. The detection of target DNA is realized by direct measurement of ECL emission of TBR. It can quantitatively detect target nucleic acids with high speed and sensitivity. This method can be used to quantitatively detect GMO fragments from real GMO products. PMID:18386909

PCR-free quantitative detection of genetically modified organism from raw materials. An electrochemiluminescence-based bio bar code method.

PubMed

Zhu, Debin; Tang, Yabing; Xing, Da; Chen, Wei R

2008-05-15

A bio bar code assay based on oligonucleotide-modified gold nanoparticles (Au-NPs) provides a PCR-free method for quantitative detection of nucleic acid targets. However, the current bio bar code assay requires lengthy experimental procedures including the preparation and release of bar code DNA probes from the target-nanoparticle complex and immobilization and hybridization of the probes for quantification. Herein, we report a novel PCR-free electrochemiluminescence (ECL)-based bio bar code assay for the quantitative detection of genetically modified organism (GMO) from raw materials. It consists of tris-(2,2'-bipyridyl) ruthenium (TBR)-labeled bar code DNA, nucleic acid hybridization using Au-NPs and biotin-labeled probes, and selective capture of the hybridization complex by streptavidin-coated paramagnetic beads. The detection of target DNA is realized by direct measurement of ECL emission of TBR. It can quantitatively detect target nucleic acids with high speed and sensitivity. This method can be used to quantitatively detect GMO fragments from real GMO products.

Sensitive electrochemical sensing platform for microRNAs detection based on shortened multi-walled carbon nanotubes with high-loaded thionin.

PubMed

Deng, Keqin; Liu, Xinyan; Li, Chunxiang; Huang, Haowen

2018-05-31

The loading capacity of thionin (Thi) on shortened multi-walled carbon nanotubes (S-MWCNTs) and acidified multi-walled carbon nanotubes (A-MWCNTs) was compared. Two DNA probe fragments were designed for hybridization with microRNA-21 (miR-21), the microRNAs (miRNAs) model analyte. DNA probe 1 (P1) was assembled on Au nanoparticles (AuNPs) modified electrode. MiR-21 was captured by the pre-immobilized P1. A signal nanoprobe was synthesized by loading large amount of Thi on S-MWCNTs with covalently bonded probe 2 (P2). Owing to the large effective surface area of MWCNTs, fast electron shuttle of MWCNTs, high-loaded Thi on S-MWCNTs, and the increased conductivity from AuNPs, after signal probe hybridized with miR-21, it gave rise to a magnified current response on electrode. The increased electrochemical current enabled us to quantitatively detect miR-21. Expensive bioreagents and labeled target/detection DNA or miRNAs were avoided in this strategy. The operation complexity and assay cost were also reduced. Copyright © 2018 Elsevier B.V. All rights reserved.

Using Experiment and Computer Modeling to Determine the Off-Axis Magnetic Field of a Solenoid

ERIC Educational Resources Information Center

Lietor-Santos, Juan Jose

2014-01-01

The study of the ideal solenoid is a common topic among introductory-based physics textbooks and a typical current arrangement in laboratory hands-on experiences where the magnetic field inside a solenoid is determined at different currents and at different distances from its center using a magnetic probe. It additionally provides a very simple…

Current crowding mediated large contact noise in graphene field-effect transistors

PubMed Central

Karnatak, Paritosh; Sai, T. Phanindra; Goswami, Srijit; Ghatak, Subhamoy; Kaushal, Sanjeev; Ghosh, Arindam

2016-01-01

The impact of the intrinsic time-dependent fluctuations in the electrical resistance at the graphene–metal interface or the contact noise, on the performance of graphene field-effect transistors, can be as adverse as the contact resistance itself, but remains largely unexplored. Here we have investigated the contact noise in graphene field-effect transistors of varying device geometry and contact configuration, with carrier mobility ranging from 5,000 to 80,000 cm2 V−1 s−1. Our phenomenological model for contact noise because of current crowding in purely two-dimensional conductors confirms that the contacts dominate the measured resistance noise in all graphene field-effect transistors in the two-probe or invasive four-probe configurations, and surprisingly, also in nearly noninvasive four-probe (Hall bar) configuration in the high-mobility devices. The microscopic origin of contact noise is directly linked to the fluctuating electrostatic environment of the metal–channel interface, which could be generic to two-dimensional material-based electronic devices. PMID:27929087

Current crowding mediated large contact noise in graphene field-effect transistors

NASA Astrophysics Data System (ADS)

Karnatak, Paritosh; Sai, T. Phanindra; Goswami, Srijit; Ghatak, Subhamoy; Kaushal, Sanjeev; Ghosh, Arindam

2016-12-01

The impact of the intrinsic time-dependent fluctuations in the electrical resistance at the graphene-metal interface or the contact noise, on the performance of graphene field-effect transistors, can be as adverse as the contact resistance itself, but remains largely unexplored. Here we have investigated the contact noise in graphene field-effect transistors of varying device geometry and contact configuration, with carrier mobility ranging from 5,000 to 80,000 cm2 V-1 s-1. Our phenomenological model for contact noise because of current crowding in purely two-dimensional conductors confirms that the contacts dominate the measured resistance noise in all graphene field-effect transistors in the two-probe or invasive four-probe configurations, and surprisingly, also in nearly noninvasive four-probe (Hall bar) configuration in the high-mobility devices. The microscopic origin of contact noise is directly linked to the fluctuating electrostatic environment of the metal-channel interface, which could be generic to two-dimensional material-based electronic devices.

X-Ray Probes of Cosmic Star-Formation History

NASA Technical Reports Server (NTRS)

Ghosh, Pranab; White, Nicholas E.

2001-01-01

In a previous paper we point out that the X-ray luminosity L(sub x) of a galaxy is driven by the evolution of its X-ray binary population and that the profile of L(sub x) with redshift can both serve as a diagnostic probe of the Star Formation Rate (SFR) profile and constrain evolutionary models for X-ray binaries. We update our previous work using a suite of more recently developed SFR profiles that span the currently plausible range. The first Chandra deep imaging results on L(sub x)-evolution are beginning to probe the SFR profile of bright spirals and the early results are consistent with predictions based on current SFR models. Using these new SFR profiles the resolution of the "birthrate problem" of lowmass X-ray binaries (LMXBs) and recycled, millisecond pulsars in terms of an evolving global SFR is more complete. We also discuss the possible impact of the variations in the SFR profile of individual galaxies.

Electroconvergent cautery.

PubMed

Patil, A A; Yamanashi, W

1994-10-01

A new cautery system known as the electroconvergent cautery system is described. This cautery system uses a radiofrequency current of 13.56 MHz. The current is passed through an amplifier, an impedance matching system, and a loading and tuning coil into a probe. After the probe tip is touched to the tissue, the mismatch of impedance between the probe tip and the generator is minimized to almost zero, resulting in high-current density at the probe tip, which causes pinpoint heating of the tissue at the point where it is touched by the probe without spread of heat to the surrounding tissue. This heat can cut and vaporize tissue and coagulate vessels. The probe can also be used to coagulate vessels with standard surgical forceps. Because convergence of energy is used for tissue heating, a grounding pad is not needed. The system can also be used under cerebrospinal fluid or saline.

Quantitative impedance measurements for eddy current model validation

NASA Astrophysics Data System (ADS)

Khan, T. A.; Nakagawa, N.

2000-05-01

This paper reports on a series of laboratory-based impedance measurement data, collected by the use of a quantitatively accurate, mechanically controlled measurement station. The purpose of the measurement is to validate a BEM-based eddy current model against experiment. We have therefore selected two "validation probes," which are both split-D differential probes. Their internal structures and dimensions are extracted from x-ray CT scan data, and thus known within the measurement tolerance. A series of measurements was carried out, using the validation probes and two Ti-6Al-4V block specimens, one containing two 1-mm long fatigue cracks, and the other containing six EDM notches of a range of sizes. Motor-controlled XY scanner performed raster scans over the cracks, with the probe riding on the surface with a spring-loaded mechanism to maintain the lift off. Both an impedance analyzer and a commercial EC instrument were used in the measurement. The probes were driven in both differential and single-coil modes for the specific purpose of model validation. The differential measurements were done exclusively by the eddyscope, while the single-coil data were taken with both the impedance analyzer and the eddyscope. From the single-coil measurements, we obtained the transfer function to translate the voltage output of the eddyscope into impedance values, and then used it to translate the differential measurement data into impedance results. The presentation will highlight the schematics of the measurement procedure, a representative of raw data, explanation of the post data-processing procedure, and then a series of resulting 2D flaw impedance results. A noise estimation will be given also, in order to quantify the accuracy of these measurements, and to be used in probability-of-detection estimation.—This work was supported by the NSF Industry/University Cooperative Research Program.

The Sheath-less Planar Langmuir Probe

NASA Astrophysics Data System (ADS)

Cooke, D. L.

2017-12-01

The Langmuir probe is one of the oldest plasma diagnostics, provided the plasma density and species temperature from analysis of a current-voltage curve as the voltage is swept over a practically chosen range. The analysis depends on a knowledge or theory of the many factors that influence the current-voltage curve including, probe shape, size, nearby perturbations, and the voltage reference. For applications in Low Earth Orbit, the Planar Langmuir Probe, PLP, is an attractive geometry because the ram ion current is very constant over many Volts of a sweep, allowing the ion density and electron temperature to be determined independently with the same instrument, at different points on the sweep. However, when the physical voltage reference is itself small and electrically floating as with a small spacecraft, the spacecraft and probe system become a double probe where the current collection theory depends on the interaction of the spacecraft with the plasma which is generally not as simple as the probe itself. The Sheath-less PLP, SPLP, interlaces on a single ram facing surface, two variably biased probe elements, broken into many small and intertwined segments on a scale smaller than the plasma Debye length. The SPLP is electrically isolated from the rest of the spacecraft. For relative bias potentials of a few volts, the ion current to all segments of each element will be constant, while the electron currents will vary as a function of the element potential and the electron temperature. Because the segments are small, intertwined, and floating, the assembly will always present the same floating potential to the plasma, with minimal growth as a function of voltage, thus sheath-less and still planar. This concept has been modelled with Nascap, and tested with a physical model inserted into a Low Earth Orbit-like chamber plasma. Results will be presented.

Impedance properties of circular microstrip antenna

NASA Technical Reports Server (NTRS)

Deshpande, M. D.; Bailey, M. C.

1983-01-01

A moment method solution to the input impedance of a circular microstrip antenna excited by either a microstrip feed or a coaxial probe is presented. Using the exact dyadic Green's function and the Fourier transform the problem is formulated in terms of Richmond's reaction integral equation from which the unknown patch current can be solved for. The patch current is expanded in terms of regular surface patch modes and an attachment mode (for probe excited case) which insures continuity of the current at probe/patch junction, proper polarization and p-dependance of patch current in the vicinity of the probe. The input impedance of a circular microstrip antenna is computed and compared with earlier results. Effect of attachment mode on the input impedance is also discussed.

Integrated circuit electrometer and sweep circuitry for an atmospheric probe

NASA Technical Reports Server (NTRS)

Zimmerman, L. E.

1971-01-01

The design of electrometer circuitry using an integrated circuit operational amplifier with a MOSFET input is described. Input protection against static voltages is provided by a dual ultra low leakage diode or a neon lamp. Factors affecting frequency response leakage resistance, and current stability are discussed, and methods are suggested for increasing response speed and for eliminating leakage resistance and current instabilities. Based on the above, two practical circuits, one having a linear response and the other a logarithmic response, were designed and evaluated experimentally. The design of a sweep circuit to implement mobility measurements using atmospheric probes is presented. A triangular voltage waveform is generated and shaped to contain a step in voltage from zero volts in both positive and negative directions.

Sensitive detection of microRNAs based on the conversion of colorimetric assay into electrochemical analysis with duplex-specific nuclease-assisted signal amplification

PubMed Central

Xia, Ning; Liu, Ke; Zhou, Yingying; Li, Yuanyuan; Yi, Xinyao

2017-01-01

miRNAs have emerged as new biomarkers for the detection of a wide variety of cancers. By employing duplex-specific nuclease for signal amplification and gold nanoparticles (AuNPs) as the carriers of detection probes, a novel electrochemical assay of miRNAs was performed. The method is based on conversion of the well-known colorimetric assay into electrochemical analysis with enhanced sensitivity. DNA capture probes immobilized on the electrode surface and ferrocene (Fc)-labeled DNA detection probes (denoted “Fc-DNA-Fc”) presented in the solution induced the assembly of positively charged AuNPs on the electrode surface through the electrostatic interaction. As a result, a large number of Fc-DNA-Fc molecules were attached on the electrode surface, thus amplifying the electrochemical signal. When duplex-specific nuclease was added to recycle the process of miRNA-initiated digestion of the immobilized DNA probes, Fc-DNA-Fc-induced assembly of AuNPs on the electrode surface could not occur. This resulted in a significant fall in the oxidation current of Fc. The current was found to be inversely proportional to the concentration of miRNAs in the range of 0–25 fM, and a detection limit of 0.1 fM was achieved. Moreover, this work presents a new method for converting colorimetric assays into sensitive electrochemical analyses, and thus would be valuable for design of novel chemical/biosensors. PMID:28761341

A Common Probe Design for Multiple Planetary Destinations

NASA Technical Reports Server (NTRS)

Hwang, H. H.; Allen, G. A., Jr.; Alunni, A. I.; Amato, M. J.; Atkinson, D. H.; Bienstock, B. J.; Cruz, J. R.; Dillman, R. A.; Cianciolo, A. D.; Elliott, J. O.;

Recent and Future Enhancements in NDI for Aircraft Structures

DTIC Science & Technology

2015-11-30

accomplish NDI of aircraft structure. This includes improved eddy current probes, improved eddy current instrumentation, as well as other...Aircraft Structures,” which is currently in Revision C [8]. The document divides various inspection methods, such as eddy current and fluorescent...efforts at AFRL to address technology shortfalls include improved eddy current probes, improved eddy current instrumentation, as well as other

Recent and Future Enhancements in NDI for Aircraft Structures (Postprint)

DTIC Science & Technology

2015-11-30

accomplish NDI of aircraft structure. This includes improved eddy current probes, improved eddy current instrumentation, as well as other...Aircraft Structures,” which is currently in Revision C [8]. The document divides various inspection methods, such as eddy current and fluorescent...efforts at AFRL to address technology shortfalls include improved eddy current probes, improved eddy current instrumentation, as well as other

Recent and Future Enhancements in NDI for Aircraft Structures (Postprint)

DTIC Science & Technology

2015-11-01

accomplish NDI of aircraft structure. This includes improved eddy current probes, improved eddy current instrumentation, as well as other...Aircraft Structures,” which is currently in Revision C [8]. The document divides various inspection methods, such as eddy current and fluorescent...efforts at AFRL to address technology shortfalls include improved eddy current probes, improved eddy current instrumentation, as well as other

Recent and Future Enhancements in NDI for Aircraft Structures (POSTPRINT)

DTIC Science & Technology

2015-11-16

accomplish NDI of aircraft structure. This includes improved eddy current probes, improved eddy current instrumentation, as well as other...Aircraft Structures,” which is currently in Revision C [8]. The document divides various inspection methods, such as eddy current and fluorescent...efforts at AFRL to address technology shortfalls include improved eddy current probes, improved eddy current instrumentation, as well as other

Electrochemical latent redox ratiometric probes for real-time tracking and quantification of endogenous hydrogen sulfide production in living cells.

PubMed

Manibalan, Kesavan; Mani, Veerappan; Chang, Pu-Chieh; Huang, Chih-Hung; Huang, Sheng-Tung; Marchlewicz, Kasper; Neethirajan, Suresh

2017-10-15

Hydrogen sulfide (H 2 S) was discovered as a third gasotransmitter in biological systems and recent years have seen a growing interest to understand its physiological and pathological functions. However, one major limiting factor is the lack of robust sensors to quantitatively track its production in real-time. We described a facile electrochemical assay based on latent redox probe approach for highly specific and sensitive quantification in living cells. Two chemical probes, Azido Benzyl ferrocene carbamate (ABFC) and N-alkyl Azido Benzyl ferrocene carbamate (NABFC) composed of azide trigger group were designed. H 2 S molecules specifically triggered the release of reporters from probes and the current response was monitored using graphene oxide film modified electrode as transducer. The detection limits are 0.32µM (ABFC) and 0.076µM (NABFC) which are comparable to those of current sensitive methods. The probes are successful in the determination of H 2 S spiked in whole human blood, fetal bovine serum, and E. coli. The continuous monitoring and quantification of endogenous H 2 S production in E. coli were successfully accomplished. This work lays first step stone towards real-time electrochemical quantification of endogenous H 2 S in living cells, thus hold great promise in the analytical aspects of H 2 S. Copyright © 2017 Elsevier B.V. All rights reserved.

Improving the psychometric properties of dot-probe attention measures using response-based computation.

PubMed

Evans, Travis C; Britton, Jennifer C

2018-09-01

Abnormal threat-related attention in anxiety disorders is most commonly assessed and modified using the dot-probe paradigm; however, poor psychometric properties of reaction-time measures may contribute to inconsistencies across studies. Typically, standard attention measures are derived using average reaction-times obtained in experimentally-defined conditions. However, current approaches based on experimentally-defined conditions are limited. In this study, the psychometric properties of a novel response-based computation approach to analyze dot-probe data are compared to standard measures of attention. 148 adults (19.19 ± 1.42 years, 84 women) completed a standardized dot-probe task including threatening and neutral faces. We generated both standard and response-based measures of attention bias, attentional orientation, and attentional disengagement. We compared overall internal consistency, number of trials necessary to reach internal consistency, test-retest reliability (n = 72), and criterion validity obtained using each approach. Compared to standard attention measures, response-based measures demonstrated uniformly high levels of internal consistency with relatively few trials and varying improvements in test-retest reliability. Additionally, response-based measures demonstrated specific evidence of anxiety-related associations above and beyond both standard attention measures and other confounds. Future studies are necessary to validate this approach in clinical samples. Response-based attention measures demonstrate superior psychometric properties compared to standard attention measures, which may improve the detection of anxiety-related associations and treatment-related changes in clinical samples. Copyright © 2018 Elsevier Ltd. All rights reserved.

Local noise in a diffusive conductor

PubMed Central

Tikhonov, E. S.; Shovkun, D. V.; Ercolani, D.; Rossella, F.; Rocci, M.; Sorba, L.; Roddaro, S.; Khrapai, V. S.

2016-01-01

The control and measurement of local non-equilibrium configurations is of utmost importance in applications on energy harvesting, thermoelectrics and heat management in nano-electronics. This challenging task can be achieved with the help of various local probes, prominent examples including superconducting or quantum dot based tunnel junctions, classical and quantum resistors, and Raman thermography. Beyond time-averaged properties, valuable information can also be gained from spontaneous fluctuations of current (noise). From these perspective, however, a fundamental constraint is set by current conservation, which makes noise a characteristic of the whole conductor, rather than some part of it. Here we demonstrate how to remove this obstacle and pick up a local noise temperature of a current biased diffusive conductor with the help of a miniature noise probe. This approach is virtually noninvasive for the electronic energy distributions and extends primary local measurements towards strongly non-equilibrium regimes. PMID:27466216

Local noise in a diffusive conductor

NASA Astrophysics Data System (ADS)

Tikhonov, E. S.; Shovkun, D. V.; Ercolani, D.; Rossella, F.; Rocci, M.; Sorba, L.; Roddaro, S.; Khrapai, V. S.

2016-07-01

The control and measurement of local non-equilibrium configurations is of utmost importance in applications on energy harvesting, thermoelectrics and heat management in nano-electronics. This challenging task can be achieved with the help of various local probes, prominent examples including superconducting or quantum dot based tunnel junctions, classical and quantum resistors, and Raman thermography. Beyond time-averaged properties, valuable information can also be gained from spontaneous fluctuations of current (noise). From these perspective, however, a fundamental constraint is set by current conservation, which makes noise a characteristic of the whole conductor, rather than some part of it. Here we demonstrate how to remove this obstacle and pick up a local noise temperature of a current biased diffusive conductor with the help of a miniature noise probe. This approach is virtually noninvasive for the electronic energy distributions and extends primary local measurements towards strongly non-equilibrium regimes.

Four-point probe measurements using current probes with voltage feedback to measure electric potentials

NASA Astrophysics Data System (ADS)

Lüpke, Felix; Cuma, David; Korte, Stefan; Cherepanov, Vasily; Voigtländer, Bert

2018-02-01

We present a four-point probe resistance measurement technique which uses four equivalent current measuring units, resulting in minimal hardware requirements and corresponding sources of noise. Local sample potentials are measured by a software feedback loop which adjusts the corresponding tip voltage such that no current flows to the sample. The resulting tip voltage is then equivalent to the sample potential at the tip position. We implement this measurement method into a multi-tip scanning tunneling microscope setup such that potentials can also be measured in tunneling contact, allowing in principle truly non-invasive four-probe measurements. The resulting measurement capabilities are demonstrated for \

Eddy current position indicating apparatus for measuring displacements of core components of a liquid metal nuclear reactor

DOEpatents

Day, Clifford K.; Stringer, James L.

1977-01-01

Apparatus for measuring displacements of core components of a liquid metal fast breeder reactor by means of an eddy current probe. The active portion of the probe is located within a dry thimble which is supported on a stationary portion of the reactor core support structure. Split rings of metal, having a resistivity significantly different than sodium, are fixedly mounted on the core component to be monitored. The split rings are slidably positioned around, concentric with the probe and symmetrically situated along the axis of the probe so that motion of the ring along the axis of the probe produces a proportional change in the probes electrical output.

Rotating concave eddy current probe

DOEpatents

Roach, Dennis P [Albuquerque, NM; Walkington, Phil [Albuquerque, NM; Rackow, Kirk A [Albuquerque, NM; Hohman, Ed [Albuquerque, NM

2008-04-01

A rotating concave eddy current probe for detecting fatigue cracks hidden from view underneath the head of a raised head fastener, such as a buttonhead-type rivet, used to join together structural skins, such as aluminum aircraft skins. The probe has a recessed concave dimple in its bottom surface that closely conforms to the shape of the raised head. The concave dimple holds the probe in good alignment on top of the rivet while the probe is rotated around the rivet's centerline. One or more magnetic coils are rigidly embedded within the probe's cylindrical body, which is made of a non-conducting material. This design overcomes the inspection impediment associated with widely varying conductivity in fastened joints.

Self-Nulling Eddy Current Probe for Surface and Subsurface Flaw Detection

NASA Technical Reports Server (NTRS)

Wincheski, B.; Fulton, J. P.; Nath, S.; Namkung, M.; Simpson, J. W.

1994-01-01

An eddy current probe which provides a null-signal in the presence of unflawed material without the need for any balancing circuitry has been developed at NASA Langley Research Center. Such a unique capability of the probe reduces set-up time, eliminates tester configuration errors, and decreases instrumentation requirements. The probe is highly sensitive to surface breaking fatigue cracks, and shows excellent resolution for the measurement of material thickness, including material loss due to corrosion damage. The presence of flaws in the material under test causes an increase in the extremely stable and reproducible output voltage of the probe. The design of the probe and some examples illustrating its flaw detection capabilities are presented.

Unified Ultrasonic/Eddy-Current Data Acquisition

NASA Technical Reports Server (NTRS)

Chern, E. James; Butler, David W.

1993-01-01

Imaging station for detecting cracks and flaws in solid materials developed combining both ultrasonic C-scan and eddy-current imaging. Incorporation of both techniques into one system eliminates duplication of computers and of mechanical scanners; unifies acquisition, processing, and storage of data; reduces setup time for repetitious ultrasonic and eddy-current scans; and increases efficiency of system. Same mechanical scanner used to maneuver either ultrasonic or eddy-current probe over specimen and acquire point-by-point data. For ultrasonic scanning, probe linked to ultrasonic pulser/receiver circuit card, while, for eddy-current imaging, probe linked to impedance-analyzer circuit card. Both ultrasonic and eddy-current imaging subsystems share same desktop-computer controller, containing dedicated plug-in circuit boards for each.

Eddy current inspection of graphite fiber components

NASA Technical Reports Server (NTRS)

Workman, G. L.; Bryson, C. C.

1990-01-01

The recognition of defects in materials properties still presents a number of problems for nondestructive testing in aerospace systems. This project attempts to utilize current capabilities in eddy current instrumentation, artificial intelligence, and robotics in order to provide insight into defining geometrical aspects of flaws in composite materials which are capable of being evaluated using eddy current inspection techniques. The unique capabilities of E-probes and horseshoe probes for inspecting probes for inspecting graphite fiber materials were evaluated and appear to hold great promise once the technology development matures. The initial results are described of modeling eddy current interactions with certain flaws in graphite fiber samples.

NanoCluster Beacons as reporter probes in rolling circle enhanced enzyme activity detection

NASA Astrophysics Data System (ADS)

Juul, Sissel; Obliosca, Judy M.; Liu, Cong; Liu, Yen-Liang; Chen, Yu-An; Imphean, Darren M.; Knudsen, Birgitta R.; Ho, Yi-Ping; Leong, Kam W.; Yeh, Hsin-Chih

2015-04-01

As a newly developed assay for the detection of endogenous enzyme activity at the single-catalytic-event level, Rolling Circle Enhanced Enzyme Activity Detection (REEAD) has been used to measure enzyme activity in both single human cells and malaria-causing parasites, Plasmodium sp. Current REEAD assays rely on organic dye-tagged linear DNA probes to report the rolling circle amplification products (RCPs), the cost of which may hinder the widespread use of REEAD. Here we show that a new class of activatable probes, NanoCluster Beacons (NCBs), can simplify the REEAD assays. Easily prepared without any need for purification and capable of large fluorescence enhancement upon hybridization, NCBs are cost-effective and sensitive. Compared to conventional fluorescent probes, NCBs are also more photostable. As demonstrated in reporting the human topoisomerases I (hTopI) cleavage-ligation reaction, the proposed NCBs suggest a read-out format attractive for future REEAD-based diagnostics.As a newly developed assay for the detection of endogenous enzyme activity at the single-catalytic-event level, Rolling Circle Enhanced Enzyme Activity Detection (REEAD) has been used to measure enzyme activity in both single human cells and malaria-causing parasites, Plasmodium sp. Current REEAD assays rely on organic dye-tagged linear DNA probes to report the rolling circle amplification products (RCPs), the cost of which may hinder the widespread use of REEAD. Here we show that a new class of activatable probes, NanoCluster Beacons (NCBs), can simplify the REEAD assays. Easily prepared without any need for purification and capable of large fluorescence enhancement upon hybridization, NCBs are cost-effective and sensitive. Compared to conventional fluorescent probes, NCBs are also more photostable. As demonstrated in reporting the human topoisomerases I (hTopI) cleavage-ligation reaction, the proposed NCBs suggest a read-out format attractive for future REEAD-based diagnostics. Electronic supplementary information (ESI) available: The detailed steps of NCB preparation, REEAD assay and STEM imaging. The sequences of the sNCB and the REEAD substrate. See DOI: 10.1039/c5nr01705j

Capacitance-level/density monitor for fluidized-bed combustor

DOEpatents

Fasching, George E.; Utt, Carroll E.

1982-01-01

A multiple segment three-terminal type capacitance probe with segment selection, capacitance detection and compensation circuitry and read-out control for level/density measurements in a fluidized-bed vessel is provided. The probe is driven at a high excitation frequency of up to 50 kHz to sense quadrature (capacitive) current related to probe/vessel capacitance while being relatively insensitive to the resistance current component. Compensation circuitry is provided for generating a negative current of equal magnitude to cancel out only the resistive component current. Clock-operated control circuitry separately selects the probe segments in a predetermined order for detecting and storing this capacitance measurement. The selected segment acts as a guarded electrode and is connected to the read-out circuitry while all unselected segments are connected to the probe body, which together form the probe guard electrode. The selected probe segment capacitance component signal is directed to a corresponding segment channel sample and hold circuit dedicated to that segment to store the signal derived from that segment. This provides parallel outputs for display, computer input, etc., for the detected capacitance values. The rate of segment sampling may be varied to either monitor the dynamic density profile of the bed (high sampling rate) or monitor average bed characteristics (slower sampling rate).

Policy Discourses in Higher Education: Impact on Access and Equity

ERIC Educational Resources Information Center

Panigrahi, Jinusha

2014-01-01

The article examines the question of access to higher education (HE) in the context of unprecedented and irreversible changes in the higher education sector in the current scenario. It is based on a comparative study that seeks to probe the question of access based on a secondary data analysis using the NSSO data regarding household consumer…

Eddy-Current Inspection Of Graphite-Fiber Composites

NASA Technical Reports Server (NTRS)

Workman, G. L.; Bryson, C. C.

1993-01-01

NASA technical memorandum describes initial research on, and proposed development of, automated system for nondestructive eddy-current inspection of parts made of graphite-fiber/epoxy-matrix composite materials. Sensors in system E-shaped or U-shaped eddy-current probes like those described in "Eddy-Current Probes For Inspecting Graphite-Fiber Composites" (MFS-26129).

A theoretical and experimental investigation of cylindrical electrostatic probes at arbitrary incidence in flowing plasma

NASA Technical Reports Server (NTRS)

Jenkins, R. V.; Jones, W. L., Jr.

1974-01-01

The theory for calculating the current collected by a negatively biased cylindrical electrostatic probe at an arbitrary angle of attack in a weakley ionized flowing plasma is presented. The theory was constructed by considering both random and directed motion simultaneous with dynamic coupling of the flow properties and of the electric field of the probe. This direct approach yielded a theory that is more general than static plasma theories modified to account for flow. Theoretical calculations are compared with experimental electrostatic probe data obtained in the free stream of an arc-heated hypersonic wind tunnel. The theoretical calculations are based on flow conditions and plasma electron densities measured by an independent microwave interferometer technique. In addition, the theory is compared with laboratory and satellite data previously published by other investigators. In each case the comparison gives good agreement.

SQUID (superconducting quantum interference device) arrays for simultaneous magnetic measurements: Calibration and source localization performance

NASA Astrophysics Data System (ADS)

Kaufman, Lloyd; Williamson, Samuel J.; Costaribeiro, P.

1988-02-01

Recently developed small arrays of SQUID-based magnetic sensors can, if appropriately placed, locate the position of a confined biomagnetic source without moving the array. The authors present a technique with a relative accuracy of about 2 percent for calibrating such sensors having detection coils with the geometry of a second-order gradiometer. The effects of calibration error and magnetic noise on the accuracy of locating an equivalent current dipole source in the human brain are investigated for 5- and 7-sensor probes and for a pair of 7-sensor probes. With a noise level of 5 percent of peak signal, uncertainties of about 20 percent in source strength and depth for a 5-sensor probe are reduced to 8 percent for a pair of 7-sensor probes, and uncertainties of about 15 mm in lateral position are reduced to 1 mm, for the configuration considered.

Methods and compositions for efficient nucleic acid sequencing

DOEpatents

Drmanac, Radoje

2006-07-04

Disclosed are novel methods and compositions for rapid and highly efficient nucleic acid sequencing based upon hybridization with two sets of small oligonucleotide probes of known sequences. Extremely large nucleic acid molecules, including chromosomes and non-amplified RNA, may be sequenced without prior cloning or subcloning steps. The methods of the invention also solve various current problems associated with sequencing technology such as, for example, high noise to signal ratios and difficult discrimination, attaching many nucleic acid fragments to a surface, preparing many, longer or more complex probes and labelling more species.

Methods and compositions for efficient nucleic acid sequencing

DOEpatents

Drmanac, Radoje

2002-01-01

Disclosed are novel methods and compositions for rapid and highly efficient nucleic acid sequencing based upon hybridization with two sets of small oligonucleotide probes of known sequences. Extremely large nucleic acid molecules, including chromosomes and non-amplified RNA, may be sequenced without prior cloning or subcloning steps. The methods of the invention also solve various current problems associated with sequencing technology such as, for example, high noise to signal ratios and difficult discrimination, attaching many nucleic acid fragments to a surface, preparing many, longer or more complex probes and labelling more species.

Calibrating ion density profile measurements in ion thruster beam plasma

NASA Astrophysics Data System (ADS)

Zhang, Zun; Tang, Haibin; Ren, Junxue; Zhang, Zhe; Wang, Joseph

2016-11-01

The ion thruster beam plasma is characterized by high directed ion velocity (104 m/s) and low plasma density (1015 m-3). Interpretation of measurements of such a plasma based on classical Langmuir probe theory can yield a large experimental error. This paper presents an indirect method to calibrate ion density determination in an ion thruster beam plasma using a Faraday probe, a retarding potential analyzer, and a Langmuir probe. This new method is applied to determine the plasma emitted from a 20-cm-diameter Kaufman ion thruster. The results show that the ion density calibrated by the new method can be as much as 40% less than that without any ion current density and ion velocity calibration.

Dynamic current susceptibility as a probe of Majorana bound states in nanowire-based Josephson junctions

NASA Astrophysics Data System (ADS)

Trif, Mircea; Dmytruk, Olesia; Bouchiat, Hélène; Aguado, Ramón; Simon, Pascal

2018-02-01

We theoretically study a Josephson junction based on a semiconducting nanowire subject to a time-dependent flux bias. We establish a general density-matrix approach for the dynamical response of the Majorana junction and calculate the resulting flux-dependent susceptibility using both microscopic and effective low-energy descriptions for the nanowire. We find that the diagonal component of the susceptibility, associated with the dynamics of the Majorana state populations, dominates over the standard Kubo contribution for a wide range of experimentally relevant parameters. The diagonal term, explored, in this Rapid Communication, in the context of Majorana physics, allows probing accurately the presence of Majorana bound states in the junction.

Layer-by-layer assembled fluorescent probes in the second near-infrared window for systemic delivery and detection of ovarian cancer

PubMed Central

Dang, Xiangnan; Gu, Li; Qi, Jifa; Correa, Santiago; Zhang, Geran; Belcher, Angela M.; Hammond, Paula T.

2016-01-01

Fluorescence imaging in the second near-infrared window (NIR-II, 1,000–1,700 nm) features deep tissue penetration, reduced tissue scattering, and diminishing tissue autofluorescence. Here, NIR-II fluorescent probes, including down-conversion nanoparticles, quantum dots, single-walled carbon nanotubes, and organic dyes, are constructed into biocompatible nanoparticles using the layer-by-layer (LbL) platform due to its modular and versatile nature. The LbL platform has previously been demonstrated to enable incorporation of diagnostic agents, drugs, and nucleic acids such as siRNA while providing enhanced blood plasma half-life and tumor targeting. This work carries out head-to-head comparisons of currently available NIR-II probes with identical LbL coatings with regard to their biodistribution, pharmacokinetics, and toxicities. Overall, rare-earth-based down-conversion nanoparticles demonstrate optimal biological and optical performance and are evaluated as a diagnostic probe for high-grade serous ovarian cancer, typically diagnosed at late stage. Successful detection of orthotopic ovarian tumors is achieved by in vivo NIR-II imaging and confirmed by ex vivo microscopic imaging. Collectively, these results indicate that LbL-based NIR-II probes can serve as a promising theranostic platform to effectively and noninvasively monitor the progression and treatment of serous ovarian cancer. PMID:27114520

Probe and Sensors Development for Level Measurement of Fats, Oils and Grease in Grease Boxes

PubMed Central

Faria, José; Sousa, André; Reis, Arsénio; Filipe, Vitor; Barroso, João

2016-01-01

The wide spread of food outlets has become an environmental and sanitation infrastructure problem, due to Fats, Oils and Grease (FOG). A grease box is used at the industrials facilities to collect the FOG, in a specific time window, while its quality is good for recycling (e.g., biodiesel) and it is economically valuable. After this period, it will be disposed at a cost. For the proper management of the grease boxes, it is necessary to know the quantity of FOG inside the boxes, which is a major problem, as the boxes are sealed and permanently filled with water. The lack of homogeneity of the FOG renders it not detectable by current probes for level detection in liquids. In this article, the design, development and testing of a set of probes for FOG level measurement, based on the principles used in sensors for the detection of liquids inside containers, is described. The most suitable probe, based on the capacitance principle, together with the necessary hardware and software modules for data acquisition and transmission, was developed and tested. After the development phase, the probe was integrated on a metropolitan system for FOG collection and grease box management in partnership with a grease box management company. PMID:27649204

Probe and Sensors Development for Level Measurement of Fats, Oils and Grease in Grease Boxes.

PubMed

Faria, José; Sousa, André; Reis, Arsénio; Filipe, Vitor; Barroso, João

2016-09-16

The wide spread of food outlets has become an environmental and sanitation infrastructure problem, due to Fats, Oils and Grease (FOG). A grease box is used at the industrials facilities to collect the FOG, in a specific time window, while its quality is good for recycling (e.g., biodiesel) and it is economically valuable. After this period, it will be disposed at a cost. For the proper management of the grease boxes, it is necessary to know the quantity of FOG inside the boxes, which is a major problem, as the boxes are sealed and permanently filled with water. The lack of homogeneity of the FOG renders it not detectable by current probes for level detection in liquids. In this article, the design, development and testing of a set of probes for FOG level measurement, based on the principles used in sensors for the detection of liquids inside containers, is described. The most suitable probe, based on the capacitance principle, together with the necessary hardware and software modules for data acquisition and transmission, was developed and tested. After the development phase, the probe was integrated on a metropolitan system for FOG collection and grease box management in partnership with a grease box management company.

Materials and Manufacturing Technology Directorate Thermal Sciences and Materials Branch (Overview)

DTIC Science & Technology

2010-09-01

Molecular Mechanics for thermo-mechanical response Materials Characterization • CNT modified durable thermal interface ( DTI ) • MEMS-based RTD micro...stabilization. Surface Characterization by Atomic Force Microscopy: Probing Thermal, Electrical, and Mechanical Properties Heater Current Path Anchor Leg 50 µm

Input impedance of coaxially fed rectangular microstrip antenna on electrically thick substrate

NASA Technical Reports Server (NTRS)

Chen, Wei; Lee, Kai-Fong; Lee, R. Q.

1993-01-01

A full-wave spectral domain analysis has been used to obtain input-impedance results for a probe-fed rectangular-patch antenna, modeling the source as a magnetic-current frill. Multiple modes are used in the probe surface current to account for axial and azimuthal variations. It is established that maximum resistance is dependent on the substrate loss tangent. The axial variation of the probe current must be taken into account for substrate thicknesses greater than about 0.02 wavelengths.

Rapid diagnosis of common deletional α-thalassemia in the Chinese population by qPCR based on identical primer homologous fragments.

PubMed

Long, Ju

2016-05-01

In China, -(SEA), -α(3.7) and -α(4.2) are common deletional α-thalassemia alleles. Gap-PCR is the currently used detection method for these alleles, whose disadvantages include time-consuming procedure and increased potential for PCR product contamination. Therefore, this detection method needs to be improved. Based on identical-primer homologous fragments, a qPCR system was developed for deletional α-thalassemia genotyping, which was composed of a group of quantitatively-related primers and their corresponding probes plus two groups of qualitatively-related primers and their corresponding probes. In order to verify the accuracy of the qPCR system, known genotype samples and random samples are employed. The standard curve result demonstrated that designed primers and probes all yielded good amplification efficiency. In the tests of known genotype samples and random samples, sample detection results were consistent with verification results. In detecting αα, -(SEA), -α(3.7) and -α(4.2) alleles, deletional α-thalassemia alleles are accurately detected by this method. In addition, this method is provided with a wider detection range, greater speed and reduced PCR product contamination risk when compared with current common gap-PCR detection reagents. Copyright © 2016 Elsevier B.V. All rights reserved.

Advanced electric-field scanning probe lithography on molecular resist using active cantilever

NASA Astrophysics Data System (ADS)

Kaestner, Marcus; Aydogan, Cemal; Ivanov, Tzvetan; Ahmad, Ahmad; Angelov, Tihomir; Reum, Alexander; Ishchuk, Valentyn; Krivoshapkina, Yana; Hofer, Manuel; Lenk, Steve; Atanasov, Ivaylo; Holz, Mathias; Rangelow, Ivo W.

2015-07-01

The routine "on demand" fabrication of features smaller than 10 nm opens up new possibilities for the realization of many devices. Driven by the thermally actuated piezoresistive cantilever technology, we have developed a prototype of a scanning probe lithography (SPL) platform which is able to image, inspect, align, and pattern features down to the single digit nanoregime. Here, we present examples of practical applications of the previously published electric-field based current-controlled scanning probe lithography. In particular, individual patterning tests are carried out on calixarene by using our developed table-top SPL system. We have demonstrated the application of a step-and-repeat SPL method including optical as well as atomic force microscopy-based navigation and alignment. The closed-loop lithography scheme was applied to sequentially write positive and negative tone features. Due to the integrated unique combination of read-write cycling, each single feature is aligned separately with the highest precision and inspected after patterning. This routine was applied to create a pattern step by step. Finally, we have demonstrated the patterning over larger areas, over existing topography, and the practical applicability of the SPL processes for lithography down to 13-nm pitch patterns. To enhance the throughput capability variable beam diameter electric field, current-controlled SPL is briefly discussed.

Eddy-Current Inspection Of Tab Seals On Beverage Cans

NASA Technical Reports Server (NTRS)

Bar-Cohen, Yoseph

1994-01-01

Eddy-current inspection system monitors tab seals on beverage cans. Device inspects all cans at usual production rate of 1,500 to 2,000 cans per minute. Automated inspection of all units replaces visual inspection by microscope aided by mass spectrometry. System detects defects in real time. Sealed cans on conveyor pass near one of two coils in differential eddy-current probe. Other coil in differential eddy-current probe positioned near stationary reference can on which tab seal is known to be of acceptable quality. Signal of certain magnitude at output of probe indicates defective can, automatically ejected from conveyor.

Detection of Fatigue Cracks at Rivets with Self-Nulling Probe

NASA Technical Reports Server (NTRS)

Wincheski, Buzz; Fulton, Jim; Nath, Shridhar; Namkung, Min

1994-01-01

A new eddy current probe developed at NASA Langley Research Center has been used to detect small cracks at rivets in aircraft lap splices [1]. The device has earlier been used to detect isolated fatigue cracks with a minimum detectable flaw size of roughly 1/2 to 1/3 the diameter of the probe [2]. The present work shows that the detectable flaw size for cracks originating at rivets can be greatly improved upon from that of isolated flaws. The use of a rotating probe method combined with spatial filtering has been used to detect 0.18 cm EDM notches, as measured from the rivet shank, with a 1.27 cm diameter probe and to detect flaws buried under the rivet head, down to a length of 0.076 cm, using a 0.32 cm diameter probe. The Self-Nulling Electromagnetic Flaw Detector induces a high density eddy current ring in the sample under test. A ferromagnetic flux focusing lens is incorporated such that in the absence of any inhomogeneities in the material under test only a minimal magnetic field will reach the interior of the probe. A magnetometer (pickup coil) located in the center of the probe therefore registers a null voltage in the absence of material defects. When a fatigue crack or other discontinuity is present in the test article the path of the eddy currents in the material is changed. The magnetic field associated with these eddy currents then enter into the interior of the probe, producing a large output voltage across the pickup coil leads. Further

Development of eddy current probe for fiber orientation assessment in carbon fiber composites

NASA Astrophysics Data System (ADS)

Wincheski, Russell A.; Zhao, Selina

2018-04-01

Measurement of the fiber orientation in a carbon fiber composite material is crucial in understanding the load carrying capability of the structure. As manufacturing conditions including resin flow and molding pressures can alter fiber orientation, verification of the as-designed fiber layup is necessary to ensure optimal performance of the structure. In this work, the development of an eddy current probe and data processing technique for analysis of fiber orientation in carbon fiber composites is presented. A proposed directional eddy current probe is modeled and its response to an anisotropic multi-layer conductor simulated. The modeling results are then used to finalize specifications of the eddy current probe. Experimental testing of the fabricated probe is presented for several samples including a truncated pyramid part with complex fiber orientation draped to the geometry for resin transfer molding. The inductively coupled single sided measurement enables fiber orientation characterization through the thickness of the part. The fast and cost-effective technique can be applied as a spot check or as a surface map of the fiber orientations across the structure. This paper will detail the results of the probe design, computer simulations, and experimental results.

Pinch-off mechanism in double-lateral-gate junctionless transistors fabricated by scanning probe microscope based lithography

PubMed Central

Dehzangi, Arash; Abedini, Alam; Abdullah, Ahmad Makarimi; Saion, Elias; Hutagalung, Sabar D; Hamidon, Mohd N; Hassan, Jumiah

2012-01-01

Summary A double-lateral-gate p-type junctionless transistor is fabricated on a low-doped (1015) silicon-on-insulator wafer by a lithography technique based on scanning probe microscopy and two steps of wet chemical etching. The experimental transfer characteristics are obtained and compared with the numerical characteristics of the device. The simulation results are used to investigate the pinch-off mechanism, from the flat band to the off state. The study is based on the variation of the carrier density and the electric-field components. The device is a pinch-off transistor, which is normally in the on state and is driven into the off state by the application of a positive gate voltage. We demonstrate that the depletion starts from the bottom corner of the channel facing the gates and expands toward the center and top of the channel. Redistribution of the carriers due to the electric field emanating from the gates creates an electric field perpendicular to the current, toward the bottom of the channel, which provides the electrostatic squeezing of the current. PMID:23365794

Eddy-Current Probes For Inspecting Graphite-Fiber Composites

NASA Technical Reports Server (NTRS)

Workman, Gary L.; Wang, Morgan

1992-01-01

Eddy-current probes with E-shaped and U-shaped magnetic cores developed to detect flaws in graphite-fiber/epoxy and other composites. Magnetic fields more concentrated, yielding better coupling with specimens.

Ionosphere Plasma State Determination in Low Earth Orbit from International Space Station Plasma Monitor

NASA Technical Reports Server (NTRS)

Kramer, Leonard

2014-01-01

A plasma diagnostic package is deployed on the International Space Station (ISS). The system - a Floating Potential Measurement Unit (FPMU) - is used by NASA to monitor the electrical floating potential of the vehicle to assure astronaut safety during extravehicular activity. However, data from the unit also reflects the ionosphere state and seems to represent an unutilized scientific resource in the form of an archive of scientific plasma state data. The unit comprises a Floating Potential probe and two Langmuir probes. There is also an unused but active plasma impedance probe. The data, at one second cadence, are collected, typically for a two week period surrounding extravehicular activity events. Data is also collected any time a visiting vehicle docks with ISS and also when any large solar events occur. The telemetry system is unusual because the package is mounted on a television camera stanchion and its data is impressed on a video signal that is transmitted to the ground and streamed by internet to two off center laboratory locations. The data quality has in the past been challenged by weaknesses in the integrated ground station and distribution systems. These issues, since mid-2010, have been largely resolved and the ground stations have been upgraded. Downstream data reduction has been developed using physics based modeling of the electron and ion collecting character in the plasma. Recursive algorithms determine plasma density and temperature from the raw Langmuir probe current voltage sweeps and this is made available in real time for situational awareness. The purpose of this paper is to describe and record the algorithm for data reduction and to show that the Floating probe and Langmuir probes are capable of providing long term plasma state measurement in the ionosphere. Geophysical features such as the Appleton anomaly and high latitude modulation at the edge of the Auroral zones are regularly observed in the nearly circular, 51 deg inclined, 400 km altitude ISS orbit. Evidence of waves in the ion collection current data is seen in geographic zones known to exhibit the spread-F phenomenon. An anomaly in the current collection characteristic of the cylindrical probe appears also too be organized by the geomagnetic field.

Simultaneous Nanoscale Surface Charge and Topographical Mapping.

PubMed

Perry, David; Al Botros, Rehab; Momotenko, Dmitry; Kinnear, Sophie L; Unwin, Patrick R

2015-07-28

Nanopipettes are playing an increasingly prominent role in nanoscience, for sizing, sequencing, delivery, detection, and mapping interfacial properties. Herein, the question of how to best resolve topography and surface charge effects when using a nanopipette as a probe for mapping in scanning ion conductance microscopy (SICM) is addressed. It is shown that, when a bias modulated (BM) SICM scheme is used, it is possible to map the topography faithfully, while also allowing surface charge to be estimated. This is achieved by applying zero net bias between the electrode in the SICM tip and the one in bulk solution for topographical mapping, with just a small harmonic perturbation of the potential to create an AC current for tip positioning. Then, a net bias is applied, whereupon the ion conductance current becomes sensitive to surface charge. Practically this is optimally implemented in a hopping-cyclic voltammetry mode where the probe is approached at zero net bias at a series of pixels across the surface to reach a defined separation, and then a triangular potential waveform is applied and the current response is recorded. Underpinned with theoretical analysis, including finite element modeling of the DC and AC components of the ionic current flowing through the nanopipette tip, the powerful capabilities of this approach are demonstrated with the probing of interfacial acid-base equilibria and high resolution imaging of surface charge heterogeneities, simultaneously with topography, on modified substrates.

Comparative analyses of plasma probe diagnostics techniques

DOE Office of Scientific and Technical Information (OSTI.GOV)

Godyak, V. A.; Alexandrovich, B. M.

The subject of this paper is a comparative analysis of the plasma parameters inferred from the classical Langmuir probe procedure, from different theories of the ion current to the probe, and from measured electron energy distribution function (EEDF) obtained by double differentiation of the probe characteristic. We concluded that the plasma parameters inferred from the classical Langmuir procedure can be subjected to significant inaccuracy due to the non-Maxwellian EEDF, uncertainty of locating the plasma potential, and the arbitrariness of the ion current approximation. The plasma densities derived from the ion part of the probe characteristics diverge by as much asmore » an order of magnitude from the density calculated according to Langmuir procedure or calculated as corresponding integral of the measured EEDF. The electron temperature extracted from the ion part is always subjected to uncertainty. Such inaccuracy is attributed to modification of the EEDF for fast electrons due to inelastic electron collisions, and to deficiencies in the existing ion current theories; i.e., unrealistic assumptions about Maxwellian EEDFs, underestimation of the ion collisions and the ion ambipolar drift, and discounting deformation of the one-dimensional structure of the region perturbed by the probe. We concluded that EEDF measurement is the single reliable probe diagnostics for the basic research and industrial applications of highly non-equilibrium gas discharge plasmas. Examples of EEDF measurements point up importance of examining the probe current derivatives in real time and reiterate significance of the equipment technical characteristics, such as high energy resolution and wide dynamic range.« less

Comparative analyses of plasma probe diagnostics techniques

NASA Astrophysics Data System (ADS)

Godyak, V. A.; Alexandrovich, B. M.

2015-12-01

The subject of this paper is a comparative analysis of the plasma parameters inferred from the classical Langmuir probe procedure, from different theories of the ion current to the probe, and from measured electron energy distribution function (EEDF) obtained by double differentiation of the probe characteristic. We concluded that the plasma parameters inferred from the classical Langmuir procedure can be subjected to significant inaccuracy due to the non-Maxwellian EEDF, uncertainty of locating the plasma potential, and the arbitrariness of the ion current approximation. The plasma densities derived from the ion part of the probe characteristics diverge by as much as an order of magnitude from the density calculated according to Langmuir procedure or calculated as corresponding integral of the measured EEDF. The electron temperature extracted from the ion part is always subjected to uncertainty. Such inaccuracy is attributed to modification of the EEDF for fast electrons due to inelastic electron collisions, and to deficiencies in the existing ion current theories; i.e., unrealistic assumptions about Maxwellian EEDFs, underestimation of the ion collisions and the ion ambipolar drift, and discounting deformation of the one-dimensional structure of the region perturbed by the probe. We concluded that EEDF measurement is the single reliable probe diagnostics for the basic research and industrial applications of highly non-equilibrium gas discharge plasmas. Examples of EEDF measurements point up importance of examining the probe current derivatives in real time and reiterate significance of the equipment technical characteristics, such as high energy resolution and wide dynamic range.

High-resolution disruption halo current measurements using Langmuir probes in Alcator C-Mod

NASA Astrophysics Data System (ADS)

Tinguely, R. A.; Granetz, R. S.; Berg, A.; Kuang, A. Q.; Brunner, D.; LaBombard, B.

2018-01-01

Halo currents generated during disruptions on Alcator C-Mod have been measured with Langmuir ‘rail’ probes. These rail probes are embedded in a lower outboard divertor module in a closely-spaced vertical (poloidal) array. The dense array provides detailed resolution of the spatial dependence (~1 cm spacing) of the halo current distribution in the plasma scrape-off region with high time resolution (400 kHz digitization rate). As the plasma limits on the outboard divertor plate, the contact point is clearly discernible in the halo current data (as an inversion of current) and moves vertically down the divertor plate on many disruptions. These data are consistent with filament reconstructions of the plasma boundary, from which the edge safety factor of the disrupting plasma can be calculated. Additionally, the halo current ‘footprint’ on the divertor plate is obtained and related to the halo flux width. The voltage driving halo current and the effective resistance of the plasma region through which the halo current flows to reach the probes are also investigated. Estimations of the sheath resistance and halo region resistivity and temperature are given. This information could prove useful for modeling halo current dynamics.

Measurement of proximity induced superconductivity in MoTe2

NASA Astrophysics Data System (ADS)

Wang, Wudi; Liu, Minhao; Gibson, Quinn; Cava, Â. R. J.; Ong, N. P.

MoTe2 is predicted to have type-II Weyl nodes and many of its novel transport properties have been predicted and studied. Here we reported an experiment on the superconductivity in MoTe2 induced by proximity effect. We fabricated a SQUIPT-like device on mechanical exfoliated MoTe2 micro flakes via nanofabrication. The device contains an Aluminum tunneling probe with AlOx barrier and Al contact. We measured tunneling current from probe to the sample. By fitting the differential conductance (dI/dV), we obtained the superconducting gaps in MoTe2. The dependence of gap in MoTe2 on temperature and magnetic field was measured. We also measured the current-phase relation in Al-MoTe2-Al Josephson junctions with an inductance based measurement technique.

Non-invasive current and voltage imaging techniques for integrated circuits using scanning probe microscopy. Final report, LDRD Project FY93 and FY94

DOE Office of Scientific and Technical Information (OSTI.GOV)

Campbell, A.N.; Cole, E.I. Jr.; Tangyunyong, Paiboon

This report describes the first practical, non-invasive technique for detecting and imaging currents internal to operating integrated circuits (ICs). This technique is based on magnetic force microscopy and was developed under Sandia National Laboratories` LDRD (Laboratory Directed Research and Development) program during FY 93 and FY 94. LDRD funds were also used to explore a related technique, charge force microscopy, for voltage probing of ICs. This report describes the technical work performed under this LDRD as well as the outcomes of the project in terms of publications and awards, intellectual property and licensing, synergistic work, potential future work, hiring ofmore » additional permanent staff, and benefits to DOE`s defense programs (DP).« less

Monitoring transients in low inductance circuits

DOEpatents

Guilford, Richard P.; Rosborough, John R.

1987-01-01

A pair of flat cable transmission lines are monitored for transient current spikes by using a probe connected to a current transformer by a pickup loop and monitoring the output of the current transformer. The approach utilizes a U-shaped pickup probe wherein the pair of flat cable transmission lines are received between the legs of the U-shaped probe. The U-shaped probe is preferably formed of a flat coil conductor adhered to one side of a flexible substrate. On the other side of the flexible substrate there is a copper foil shield. The copper foil shield is connected to one end of the flat conductor coil and connected to one leg of the pickup loop which passes through the current transformer. The other end of the flat conductor coil is connected to the other leg of the pickup loop.

Polyhydric polymer-functionalized fluorescent probe with enhanced aqueous solubility and specific ion recognition: A test strips-based fluorimetric strategy for the rapid and visual detection of Fe3+ ions.

PubMed

Duan, Zhiqiang; Zhang, Chunxian; Qiao, Yuchun; Liu, Fengjuan; Wang, Deyan; Wu, Mengfan; Wang, Ke; Lv, Xiaoxia; Kong, Xiangmu; Wang, Hua

2017-08-01

A polyhydric polymer-functionalized probe with enhanced aqueous solubility was designed initially by coupling 1-pyrenecarboxyaldehyde (Pyr) onto poly(vinyl alcohol) (PVA) via the one-step condensation reaction. Polyhydric PVA polymer chains could facilitate the Pyr fluorophore with largely improved aqueous solubility and especially strong cyan fluorescence. Importantly, the fluorescence of the PVA-Pyr probes could thereby be quenched specifically by Fe 3+ ions through the strong PVA-Fe 3+ interaction triggering the polymeric probe aggregation. Furthermore, a test strips-based fluorimetric method was developed with the stable and uniform probe distribution by taking advantage of the unique film-forming ability and the depression capacity of "coffee-stain" effects of PVA matrix. The as-developed test strips could allow for the rapid and visual detections of Fe 3+ ions simply by a dipping way, showing a linear concentration range of 5.00-300μM, with the detection limit of 0.73μM. Moreover, the proposed method was applied to the evaluation of Fe 3+ ions in natural water samples, showing the analysis performances better or comparable to those of current detection techniques. This test strips-based fluorimetric strategy promises the extensive applications for the rapid on-site monitoring of Fe 3+ ions in environmental water and the outdoor finding of the potential iron mines. Copyright © 2017 Elsevier B.V. All rights reserved.

Design and fabrication of a polyimide-based microelectrode array: application in neural recording and repeatable electrolytic lesion in rat brain.

PubMed

Chen, You-Yin; Lai, Hsin-Yi; Lin, Sheng-Huang; Cho, Chien-Wen; Chao, Wen-Hung; Liao, Chia-Hsin; Tsang, Siny; Chen, Yi-Fan; Lin, Si-Yue

2009-08-30

The design and testing of a new microelectrode array, the NCTU (National Chiao Tung University) probe, was presented. Evaluation results showed it has good biocompatibility, high signal-to-noise ratio (SNR: the root mean square of background noise to the average peak-to-peak amplitude of spikes) during chronic neural recordings, and high reusability for electrolytic lesions. The probe was a flexible, polyimide-based microelectrode array with a long shaft (14.9 mm in length) and 16 electrodes (5 microm-thick and 16 microm in radius); its performance in chronic in vivo recordings was examined in rodents. To improve the precision of implantation, a metallic, impact-resistant layer was sandwiched between the polyimide layers to strengthen the probe. The three-dimensional (3D) structure of electrodes fabricated by electroplating produced rough textures that increased the effective surface area. The in vitro impedance of electrodes on the NCTU probe was 2.4+/-0.52 MOmega at 1 kHz. In addition, post-surgical neural recordings of implanted NCTU probes were conducted for up to 40 days in awake, normally behaving rats. The electrodes on the NCTU probe functioned well and had a high SNR (range: 4-5) with reliable in vivo impedance (<0.7 MOmega). The electrodes were also robust enough to functionally record events, even after the anodal current (30 microA, 10s) was repeatedly applied for 60 times. With good biocompatibility, high and stable SNR for chronic recording, and high tolerance for electrolytic lesion, the NCTU probe would serve as a useful device in future neuroscience research.

Multispectral Photoacoustic Imaging of Tumor Protease Activity with a Gold Nanocage-Based Activatable Probe.

PubMed

Liu, Cheng; Li, Shiying; Gu, Yanjuan; Xiong, Huahua; Wong, Wing-Tak; Sun, Lei

2018-05-07

Tumor proteases have been recognized as significant regulators in the tumor microenvironment, but the current strategies for in vivo protease imaging have tended to focus on the development of a probe design rather than the investigation of a novel imaging strategy by leveraging the imaging technique and probe. Herein, it is the first report to investigate the ability of multispectral photoacoustic imaging (PAI) to estimate the distribution of protease cleavage sites inside living tumor tissue by using an activatable photoacoustic (PA) probe. The protease MMP-2 is selected as the target. In this probe, gold nanocages (GNCs) with an absorption peak at ~ 800 nm and fluorescent dye molecules with an absorption peak at ~ 680 nm are conjugated via a specific enzymatic peptide substrate. Upon enzymatic activation by MMP-2, the peptide substrate is cleaved and the chromophores are released. Due to the different retention speeds of large GNCs and small dye molecules, the probe alters its intrinsic absorption profile and produces a distinct change in the PA signal. A multispectral PAI technique that can distinguish different chromophores based on intrinsic PA spectral signatures is applied to estimate the signal composition changes and indicate the cleavage interaction sites. Finally, the multispectral PAI technique with the activatable probe is tested in solution, cultured cells, and a subcutaneous tumor model in vivo. Our experiment in solution with enzyme ± inhibitor, cell culture ± inhibitor, and in vivo tumor model with administration of the developed probe ± inhibitor demonstrated the probe was cleaved by the targeted enzyme. Particularly, the in vivo estimation of the cleavage site distribution was validated with the result of ex vivo immunohistochemistry analysis. This novel synergy of the multispectral PAI technique and the activatable probe is a potential strategy for the distribution estimation of tumor protease activity in vivo.

Validation of a novel attentional bias modification task: the future may be in the cards.

PubMed

Notebaert, Lies; Clarke, Patrick J F; Grafton, Ben; MacLeod, Colin

2015-02-01

Attentional bias modification (ABM) is a promising therapeutic tool aimed at changing patterns of attentional selectivity associated with heightened anxiety. A number of studies have successfully implemented ABM using the modified dot-probe task. However others have not achieved the attentional change required to achieve emotional benefits, highlighting the need for new ABM methods. The current study compared the effectiveness of a newly developed ABM task against the traditional dot-probe ABM task. The new person-identity-matching (PIM) task presented participants with virtual cards, each depicting a happy and angry person. The task encourages selective attention toward or away from threat by requiring participants to make matching judgements between two cards, based either on the identities of the happy faces, or of the angry faces. Change in attentional bias achieved by both ABM tasks was measured by a dot-probe assessment task. Their impact on emotional vulnerability was assessed by measuring negative emotional reactions to a video stressor. The PIM task succeeded in modifying attentional bias, and exerting an impact on emotional reactivity, whereas this was not the case for the dot-probe task. These results are considered in relation to the potential clinical utility of the current task in comparison to traditional ABM methodologies. Copyright © 2014 Elsevier Ltd. All rights reserved.

Nanostar probes for tip-enhanced spectroscopy

NASA Astrophysics Data System (ADS)

Kim, Woong; Kim, Nara; Park, Joon Won; Kim, Zee Hwan

2015-12-01

To overcome the current limit of tip-enhanced spectroscopy that is based on metallic nano-probes, we developed a new scanning probe with a metallic nanostar, a nanoparticle with sharp spikes. A Au nanoparticle of 5 nm was first attached to the end of a tip through DNA-DNA hybridization and mechanical pick-up. The nanoparticle was converted to a nanostar with a core diameter of ~70 nm and spike lengths between 50 nm and 80 nm through the reduction of Au3+ with ascorbic acid in the presence of Ag+. Fabrication yields of such tips exceeded 60%, and more than 80% of such tips showed a mechanical durability sufficient for use in scanning microscopy. Effectiveness of the new probes for tip-enhanced Raman scattering (TERS) and tip-enhanced fluorescence (TEF) was confirmed. The probes exhibited the necessary enhancement for TEF, and the tip-on and tip-off ratios varied between 5 and 100. This large tip-to-tip variability may arise from the uncontrolled orientation of the apexes of the spike with respect to the sample surface, which calls for further fabrication improvement. The result overall supports a new fabrication approach for the probe that is effective for tip-enhanced spectroscopy.To overcome the current limit of tip-enhanced spectroscopy that is based on metallic nano-probes, we developed a new scanning probe with a metallic nanostar, a nanoparticle with sharp spikes. A Au nanoparticle of 5 nm was first attached to the end of a tip through DNA-DNA hybridization and mechanical pick-up. The nanoparticle was converted to a nanostar with a core diameter of ~70 nm and spike lengths between 50 nm and 80 nm through the reduction of Au3+ with ascorbic acid in the presence of Ag+. Fabrication yields of such tips exceeded 60%, and more than 80% of such tips showed a mechanical durability sufficient for use in scanning microscopy. Effectiveness of the new probes for tip-enhanced Raman scattering (TERS) and tip-enhanced fluorescence (TEF) was confirmed. The probes exhibited the necessary enhancement for TEF, and the tip-on and tip-off ratios varied between 5 and 100. This large tip-to-tip variability may arise from the uncontrolled orientation of the apexes of the spike with respect to the sample surface, which calls for further fabrication improvement. The result overall supports a new fabrication approach for the probe that is effective for tip-enhanced spectroscopy. Electronic supplementary information (ESI) available: Illustrations of TERS and TEF experiments, information about the TEM images, scheme of surface preparation and peak assignments of TERS spectra. See DOI: 10.1039/c5nr06657c

Time-resolved photoinduced thermoelectric and transport currents in GaAs nanowires.

PubMed

Prechtel, Leonhard; Padilla, Milan; Erhard, Nadine; Karl, Helmut; Abstreiter, Gerhard; Fontcuberta I Morral, Anna; Holleitner, Alexander W

2012-05-09

In order to clarify the temporal interplay of the different photocurrent mechanisms occurring in single GaAs nanowire based circuits, we introduce an on-chip photocurrent pump-probe spectroscopy with a picosecond time resolution. We identify photoinduced thermoelectric, displacement, and carrier lifetime limited currents as well as the transport of photogenerated holes to the electrodes. Moreover, we show that the time-resolved photocurrent spectroscopy can be used to investigate the drift velocity of photogenerated carriers in semiconducting nanowires. Hereby, our results are relevant for nanowire-based optoelectronic and photovoltaic applications.

Aluminum nanostructures with strong visible-range SERS activity for versatile micropatterning of molecular security labels.

PubMed

Lay, Chee Leng; Koh, Charlynn Sher Lin; Wang, Jing; Lee, Yih Hong; Jiang, Ruibin; Yang, Yijie; Yang, Zhe; Phang, In Yee; Ling, Xing Yi

2018-01-03

The application of aluminum (Al)-based nanostructures for visible-range plasmonics, especially for surface-enhanced Raman scattering (SERS), currently suffers from inconsistent local electromagnetic field distributions and/or inhomogeneous distribution of probe molecules. Herein, we lithographically fabricate structurally uniform Al nanostructures which enable homogeneous adsorption of various probe molecules. Individual Al nanostructures exhibit strong local electromagnetic field enhancements, in turn leading to intense SERS activity. The average SERS enhancement factor (EF) for individual nanostructures exceeds 10 4 for non-resonant probe molecules in the visible spectrum. These Al nanostructures also retain more than 70% of their original SERS intensities after one-month storage, displaying superb stability under ambient conditions. We further achieve tunable polarization-dependent SERS responses using anisotropic Al nanostructures, facilitating the design of sophisticated SERS-based security labels. Our micron-sized security label comprises two-tier security features, including a machine-readable hybrid quick-response (QR) code overlaid with a set of ciphertexts. Our work demonstrates the versatility of Al-based structures in low-cost modern chemical nano-analytics and forgery protection.

A Suite of "Minimalist" Photo-Crosslinkers for Live-Cell Imaging and Chemical Proteomics: Case Study with BRD4 Inhibitors.

PubMed

Pan, Sijun; Jang, Se-Young; Wang, Danyang; Liew, Si Si; Li, Zhengqiu; Lee, Jun-Seok; Yao, Shao Q

2017-09-18

Affinity-based probes (AfBPs) provide a powerful tool for large-scale chemoproteomic studies of drug-target interactions. The development of high-quality probes capable of recapitulating genuine drug-target engagement, however, could be challenging. "Minimalist" photo-crosslinkers, which contain an alkyl diazirine group and a chemically tractable tag, could alleviate such challenges, but few are currently available. Herein, we have developed new alkyl diazirine-containing photo-crosslinkers with different bioorthogonal tags. They were subsequently used to create a suite of AfBPs based on GW841819X (a small molecule inhibitor of BRD4). Through in vitro and in situ studies under conditions that emulated native drug-target interactions, we have obtained better insights into how a tag might affect the probe's performance. Finally, SILAC-based chemoproteomic studies have led to the discovery of a novel off-target, APEX1. Further studies showed GW841819X binds to APEX1 and caused up-regulation of endogenous DNMT1 expression under normoxia conditions. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

The Fixed-bias Langmuir Probe on the Communication-navigation Outage Forecast System Satellite: Calibration and Validation

NASA Technical Reports Server (NTRS)

Klenzing, Jeffrey H.; Rowland, Douglas E.

2012-01-01

A fixed-bias spherical Langmuir probe is included as part of the Vector Electric Field Instrument (VEFI) suite on the Communication Navigation Outage Forecast System (CNOFS) satellite.CNOFS gathers data in the equatorial ionosphere between 400 and 860 km, where the primary constituent ions are H+ and O+. The ion current collected by the probe surface per unit plasmadensity is found to be a strong function of ion composition. The calibration of the collected current to an absolute density is discussed, and the performance of the spherical probe is compared to other in situ instruments on board the CNOFS satellite. The application of the calibration is discussed with respect to future xed-bias probes; in particular, it is demonstrated that some density fluctuations will be suppressed in the collected current if the plasma composition rapidly changes along with density. This is illustrated in the observation of plasma density enhancements on CNOFS.

Detection of Real Flaw using Uniform Eddy Current Multi-probe

NASA Astrophysics Data System (ADS)

Fukuoka, Katsuhiro; Hashimoto, Mitsuo

The establishment of the nondestructive inspection technology with plant structures has been stimulated by the recent occurrence of cracks in the nuclear power plant structures. In this research, a uniform eddy current multi-probe to apply to the complex structure and inspect the cracks at high-speed data acquisition was developed. Pick-up coils of the developed probe were arranged on a flexible printed circuit board. This probe was able to obtain clear signal for an EDM (electro-discharge machining) slit with 0.5 mm depth and distinguish EDM slits arranged at 2 mm intervals. It was confirmed that the SCC (stress corrosion cracking) of real flaw was able to be detected with developed uniform eddy current multi-probe by using the ferrite core for the exciting coil and considering the impedance matching of the exciting coil and the flaw detection device.

Prevalence of Periodontal Diseases in a Multicenter Cohort of Perinatally HIV-Infected and HIV-exposed and Uninfected Youth

PubMed Central

Ryder, Mark I.; Yao, Tzy-Jyun; Russell, Jonathan S.; Moscicki, Anna-Barbara; Shiboski, Caroline H.

2016-01-01

Aims To compare the prevalence and severity of periodontal diseases between 180 perinatally HIV-infected (PHIV) and 118 perinatally HIV-exposed and uninfected (PHEU) youth in a cross-sectional study conducted at 11 clinical sites in the United States and Puerto Rico from the Adolescent Master Protocol (AMP) study of the Pediatric HIV/AIDS cohort study (PHACS) network. Methods Several analyses were conducted, employing the current CDC/AAP classification for periodontitis and incorporating a definition of gingivitis based on a bleeding on probing threshold, and analyses based on more detailed whole mouth, intraoral regionally, site-based, and tooth-based criteria of bleeding on probing, plaque levels, pockets depths and clinical attachment levels. Results After adjusting for plaque control habits, and behavioral and sociodemographic factors, there were no significant differences in periodontal diseases between the PHIV and PHEU youth using any of these criteria. For PHIV youth, there was no significant association between parameters of periodontal disease and current HIV status. Conclusions While no significant differences in periodontal parameters were noted between the PHIV and PHEU youth, the influence of antiretroviral therapy on merits further exploration in this cohort in a longitudinal study. PMID:27801947

Probe Array Correction With Strong Target Interactions

DTIC Science & Technology

2012-08-01

exciting each probe array feed with a unit voltage source and computing the short circuit currents, ii, i = 1 , 2 , . . . , 5, at each probe array feed...that only one probe array element has unit terminal currents. In this case I2 = Îi = IN − Y NV 2 = IN − Y N [ Y is + Y N ]− 1 IN = (I − Y N [ Y is + Y...YOUR FORM TO THE ABOVE ADDRESS. 1 . REPORT DATE (DD-MM-YY) 2 . REPORT TYPE 3. DATES COVERED (From - To) August 2012 Interim 01 May 2011 – 31 May

Apparatus for measuring the local void fraction in a flowing liquid containing a gas

DOEpatents

Dunn, P.F.

1979-07-17

The local void fraction in liquid containing a gas is measured by placing an impedance-variation probe in the liquid, applying a controlled voltage or current to the probe, and measuring the probe current or voltage. A circuit for applying the one electrical parameter and measuring the other includes a feedback amplifier that minimizes the effect of probe capacitance and a digitizer to provide a clean signal. Time integration of the signal provides a measure of the void fraction, and an oscilloscope display also shows bubble size and distribution.

Apparatus for measuring the local void fraction in a flowing liquid containing a gas

DOEpatents

Dunn, Patrick F.

1981-01-01

The local void fraction in liquid containing a gas is measured by placing an impedance-variation probe in the liquid, applying a controlled voltage or current to the probe, and measuring the probe current or voltage. A circuit for applying the one electrical parameter and measuring the other includes a feedback amplifier that minimizes the effect of probe capacitance and a digitizer to provide a clean signal. Time integration of the signal provides a measure of the void fraction, and an oscilloscope display also shows bubble size and distribution.

Non-Contact EDDY Current Hole Eccentricity and Diameter Measurement

NASA Technical Reports Server (NTRS)

Chern, E. James

1998-01-01

Precision holes are among the most critical features of a mechanical component. Deviations from permissible tolerances can impede operation and result in unexpected failure. We have developed an automated non-contact eddy current hole diameter and eccentricity measuring system. The operating principle is based on the eddy current lift-off effect, which is the coil impedance as a function of the distance between the coil and the test object. An absolute eddy current probe rotates in the hole. The impedance of each angular position is acquired and input to the computer for integration and analysis. The eccentricity of the hole is the profile of the impedance as a function of angular position as compared to a straight line, an ideal hole. The diameter of the hole is the sum of the diameter of the probe and twice the distance-calibrated impedance. An eddy current image is generated by integrating angular scans for a plurality of depths between the top and bottom to display the eccentricity profile. This system can also detect and image defects in the hole. The method for non-contact eddy current hole diameter and eccentricity measurement has been granted a patent by the U.S. Patent and Trademark Office.

The evolution of ring current ion energy density and energy content during geomagnetic storms based on Van Allen Probes measurements

DOE PAGES

Zhao, H.; Li, X.; Baker, D. N.; ...

2015-08-25

Enabled by the comprehensive measurements from the Magnetic Electron Ion Spectrometer (MagEIS), Helium Oxygen Proton Electron mass spectrometer (HOPE), and Radiation Belt Storm Probes Ion Composition Experiment (RBSPICE) instruments onboard Van Allen Probes in the heart of the radiation belt, the relative contributions of ions with different energies and species to the ring current energy density and their dependence on the phases of geomagnetic storms are quantified. The results show that lower energy (<50 keV) protons enhance much more often and also decay much faster than higher-energy protons. During the storm main phase, ions with energies <50 keV contribute moremore » significantly to the ring current than those with higher energies; while the higher-energy protons dominate during the recovery phase and quiet times. The enhancements of higher-energy proton fluxes as well as energy content generally occur later than those of lower energy protons, which could be due to the inward radial diffusion. For the 29 March 2013 storm we investigated in detail that the contribution from O + is ~25% of the ring current energy content during the main phase and the majority of that comes from <50 keV O +. This indicates that even during moderate geomagnetic storms the ionosphere is still an important contributor to the ring current ions. Using the Dessler-Parker-Sckopke relation, the contributions of ring current particles to the magnetic field depression during this geomagnetic storm are also calculated. In conclusion, the results show that the measured ring current ions contribute about half of the Dst depression.« less

The evolution of ring current ion energy density and energy content during geomagnetic storms based on Van Allen Probes measurements

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhao, H.; Li, X.; Baker, D. N.

Enabled by the comprehensive measurements from the Magnetic Electron Ion Spectrometer (MagEIS), Helium Oxygen Proton Electron mass spectrometer (HOPE), and Radiation Belt Storm Probes Ion Composition Experiment (RBSPICE) instruments onboard Van Allen Probes in the heart of the radiation belt, the relative contributions of ions with different energies and species to the ring current energy density and their dependence on the phases of geomagnetic storms are quantified. The results show that lower energy (<50 keV) protons enhance much more often and also decay much faster than higher-energy protons. During the storm main phase, ions with energies <50 keV contribute moremore » significantly to the ring current than those with higher energies; while the higher-energy protons dominate during the recovery phase and quiet times. The enhancements of higher-energy proton fluxes as well as energy content generally occur later than those of lower energy protons, which could be due to the inward radial diffusion. For the 29 March 2013 storm we investigated in detail that the contribution from O + is ~25% of the ring current energy content during the main phase and the majority of that comes from <50 keV O +. This indicates that even during moderate geomagnetic storms the ionosphere is still an important contributor to the ring current ions. Using the Dessler-Parker-Sckopke relation, the contributions of ring current particles to the magnetic field depression during this geomagnetic storm are also calculated. In conclusion, the results show that the measured ring current ions contribute about half of the Dst depression.« less

Passive MHD Spectroscopy for Enabling Magnetic Reconstructions on Spherical Tokamak Plasmas at General Fusion Inc

NASA Astrophysics Data System (ADS)

O'Shea, Peter; Laberge, Michel; Mossman, Alex; Reynolds, Meritt

2017-10-01

Magnetic reconstructions on lab based plasma injectors at General Fusion relies heavily on edge magnetic (``Bdot'') probes. On plasma experiments built for field compression (PCS) tests, the number and locations of Bdot probes is limited by mechanical constraints. Additional information about the q profiles near the core in our Spector plasmas is obtained using passive MHD spectroscopy. The coaxial helicity injection (CHI) formation process naturally generates hollow current profiles and reversed shear early in each discharge. Central Ohmic heating naturally peaks the current profiles as our plasmas evolve in time, simultaneously reducing the core safety factor, q(0), and reverse shear. As the central, non-monotonic q-profile crosses rational flux surfaces, we observe transient magnetic reconnection events (MRE's) due to the double tearing mode. Modal analysis allows us to infer the q surfaces involved in each burst. The parametric dependence of the timing of MRE's allows us to estimate the continuous time evolution of the core q profile. Combining core MHD spectroscopy with edge magnetic probe measurements greatly enhances our certainty of the overall q profile.

Langmuir probe measurements in a time-fluctuating-highly ionized non-equilibrium cutting arc: analysis of the electron retarding part of the time-averaged current-voltage characteristic of the probe.

PubMed

Prevosto, L; Kelly, H; Mancinelli, B

2013-12-01

This work describes the application of Langmuir probe diagnostics to the measurement of the electron temperature in a time-fluctuating-highly ionized, non-equilibrium cutting arc. The electron retarding part of the time-averaged current-voltage characteristic of the probe was analysed, assuming that the standard exponential expression describing the electron current to the probe in collision-free plasmas can be applied under the investigated conditions. A procedure is described which allows the determination of the errors introduced in time-averaged probe data due to small-amplitude plasma fluctuations. It was found that the experimental points can be gathered into two well defined groups allowing defining two quite different averaged electron temperature values. In the low-current region the averaged characteristic was not significantly disturbed by the fluctuations and can reliably be used to obtain the actual value of the averaged electron temperature. In particular, an averaged electron temperature of 0.98 ± 0.07 eV (= 11400 ± 800 K) was found for the central core of the arc (30 A) at 3.5 mm downstream from the nozzle exit. This average included not only a time-average over the time fluctuations but also a spatial-average along the probe collecting length. The fitting of the high-current region of the characteristic using such electron temperature value together with the corrections given by the fluctuation analysis showed a relevant departure of local thermal equilibrium in the arc core.

In vivo targeted peripheral nerve imaging with a nerve-specific nanoscale magnetic resonance probe.

PubMed

Zheng, Linfeng; Li, Kangan; Han, Yuedong; Wei, Wei; Zheng, Sujuan; Zhang, Guixiang

2014-11-01

Neuroimaging plays a pivotal role in clinical practice. Currently, computed tomography (CT), magnetic resonance imaging (MRI), ultrasonography, and positron emission tomography (PET) are applied in the clinical setting as neuroimaging modalities. There is no optimal imaging modality for clinical peripheral nerve imaging even though fluorescence/bioluminescence imaging has been used for preclinical studies on the nervous system. Some studies have shown that molecular and cellular MRI (MCMRI) can be used to visualize and image the cellular and molecular level of the nervous system. Other studies revealed that there are different pathological/molecular changes in the proximal and distal sites after peripheral nerve injury (PNI). Therefore, we hypothesized that in vivo peripheral nerve targets can be imaged using MCMRI with specific MRI probes. Specific probes should have higher penetrability for the blood-nerve barrier (BNB) in vivo. Here, a functional nanometre MRI probe that is based on nerve-specific proteins as targets, specifically, using a molecular antibody (mAb) fragment conjugated to iron nanoparticles as an MRI probe, was constructed for further study. The MRI probe allows for imaging the peripheral nerve targets in vivo. Copyright © 2014 Elsevier Ltd. All rights reserved.

A new B-dot probe-based diagnostic for amplitude, polarization, and wavenumber measurements of ion cyclotron range-of frequency fields on ASDEX Upgrade

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ochoukov, R.; Bobkov, V.; Faugel, H.

2015-11-15

A new B-dot probe-based diagnostic has been installed on an ASDEX Upgrade tokamak to characterize ion cyclotron range-of frequency (ICRF) wave generation and interaction with magnetized plasma. The diagnostic consists of a field-aligned array of B-dot probes, oriented to measure fast and slow ICRF wave fields and their field-aligned wavenumber (k{sub //}) spectrum on the low field side of ASDEX Upgrade. A thorough description of the diagnostic and the supporting electronics is provided. In order to compare the measured dominant wavenumber of the local ICRF fields with the expected spectrum of the launched ICRF waves, in-air near-field measurements were performedmore » on the newly installed 3-strap ICRF antenna to reconstruct the dominant launched toroidal wavenumbers (k{sub tor}). Measurements during a strap current phasing scan in tokamak discharges reveal an upshift in k{sub //} as strap phasing is moved away from the dipole configuration. This result is the opposite of the k{sub tor} trend expected from in-air near-field measurements; however, the near-field based reconstruction routine does not account for the effect of induced radiofrequency (RF) currents in the passive antenna structures. The measured exponential increase in the local ICRF wave field amplitude is in agreement with the upshifted k{sub //}, as strap phasing moves away from the dipole configuration. An examination of discharges heated with two ICRF antennas simultaneously reveals the existence of beat waves at 1 kHz, as expected from the difference of the two antennas’ operating frequencies. Beats are observed on both the fast and the slow wave probes suggesting that the two waves are coupled outside the active antennas. Although the new diagnostic shows consistent trends between the amplitude and the phase measurements in response to changes applied by the ICRF antennas, the disagreement with the in-air near-field measurements remains. An electromagnetic model is currently under development to address this issue.« less

Interactive fluorophore and quencher pairs for labeling fluorescent nucleic acid hybridization probes.

PubMed

Marras, Salvatore A E

2008-03-01

The use of fluorescent nucleic acid hybridization probes that generate a fluorescence signal only when they bind to their target enables real-time monitoring of nucleic acid amplification assays. Real-time nucleic acid amplification assays markedly improves the ability to obtain qualitative and quantitative results. Furthermore, these assays can be carried out in sealed tubes, eliminating carryover contamination. Fluorescent nucleic acid hybridization probes are available in a wide range of different fluorophore and quencher pairs. Multiple hybridization probes, each designed for the detection of a different nucleic acid sequence and each labeled with a differently colored fluorophore, can be added to the same nucleic acid amplification reaction, enabling the development of high-throughput multiplex assays. In order to develop robust, highly sensitive and specific real-time nucleic acid amplification assays it is important to carefully select the fluorophore and quencher labels of hybridization probes. Selection criteria are based on the type of hybridization probe used in the assay, the number of targets to be detected, and the type of apparatus available to perform the assay. This article provides an overview of different aspects of choosing appropriate labels for the different types of fluorescent hybridization probes used with different types of spectrofluorometric thermal cyclers currently available.

Scanning thermo-ionic microscopy for probing local electrochemistry at the nanoscale

DOE Office of Scientific and Technical Information (OSTI.GOV)

Eshghinejad, Ahmadreza; Nasr Esfahani, Ehsan; Wang, Peiqi

2016-05-28

Conventional electrochemical characterization techniques based on voltage and current measurements only probe faradaic and capacitive rates in aggregate. In this work we develop a scanning thermo-ionic microscopy (STIM) to probe local electrochemistry at the nanoscale, based on imaging of Vegard strain induced by thermal oscillation. It is demonstrated from both theoretical analysis and experimental validation that the second harmonic response of thermally induced cantilever vibration, associated with thermal expansion, is present in all solids, whereas the fourth harmonic response, caused by local transport of mobile species, is only present in ionic materials. The origin of STIM response is further confirmedmore » by its reduced amplitude with respect to increased contact force, due to the coupling of stress to concentration of ionic species and/or electronic defects. The technique has been applied to probe Sm-doped Ceria and LiFePO{sub 4}, both of which exhibit higher concentrations of mobile species near grain boundaries. The STIM gives us a powerful method to study local electrochemistry with high sensitivity and spatial resolution for a wide range of ionic systems, as well as ability to map local thermomechanical response.« less

Development and construction of a comprehensive set of research diagnostics for the FLARE user facility

NASA Astrophysics Data System (ADS)

Yoo, Jongsoo; Jara-Almonte, J.; Majeski, S.; Frank, S.; Ji, H.; Yamada, M.

2016-10-01

FLARE (Facility for Laboratory Reconnection Experiments) will be operated as a flexible user facility, and so a complete set of research diagnostics is under development, including magnetic probe arrays, Langmuir probes, Mach probes, spectroscopic probes, and a laser interferometer. In order to accommodate the various requirements of users, large-scale (1 m), variable resolution (0.5-4 cm) magnetic probes have been designed, and are currently being prototyped. Moreover, a fully fiber-coupled laser interferometer has been designed to measure the line-integrated electron density. This fiber-coupled interferometer system will reduce the complexity of alignment processes and minimize maintenance of the system. Finally, improvements to the electrostatic probes and spectroscopic probes currently used in the Magnetic Reconnection Experiment (MRX) are discussed. The specifications of other subsystems, such as integrators and digitizers, are also presented. This work is supported by DoE Contract No. DE-AC0209CH11466.

Detection of target-probe oligonucleotide hybridization using synthetic nanopore resistive pulse sensing.

PubMed

Booth, Marsilea Adela; Vogel, Robert; Curran, James M; Harbison, SallyAnn; Travas-Sejdic, Jadranka

2013-07-15

Despite the plethora of DNA sensor platforms available, a portable, sensitive, selective and economic sensor able to rival current fluorescence-based techniques would find use in many applications. In this research, probe oligonucleotide-grafted particles are used to detect target DNA in solution through a resistive pulse nanopore detection technique. Using carbodiimide chemistry, functionalized probe DNA strands are attached to carboxylated dextran-based magnetic particles. Subsequent incubation with complementary target DNA yields a change in surface properties as the two DNA strands hybridize. Particle-by-particle analysis with resistive pulse sensing is performed to detect these changes. A variable pressure method allows identification of changes in the surface charge of particles. As proof-of-principle, we demonstrate that target hybridization is selectively detected at micromolar concentrations (nanomoles of target) using resistive pulse sensing, confirmed by fluorescence and phase analysis light scattering as complementary techniques. The advantages, feasibility and limitations of using resistive pulse sensing for sample analysis are discussed. Copyright © 2013 Elsevier B.V. All rights reserved.

All-optical optoacoustic microscopy based on probe beam deflection technique.

PubMed

Maswadi, Saher M; Ibey, Bennett L; Roth, Caleb C; Tsyboulski, Dmitri A; Beier, Hope T; Glickman, Randolph D; Oraevsky, Alexander A

2016-09-01

Optoacoustic (OA) microscopy using an all-optical system based on the probe beam deflection technique (PBDT) for detection of laser-induced acoustic signals was investigated as an alternative to conventional piezoelectric transducers. PBDT provides a number of advantages for OA microscopy including (i) efficient coupling of laser excitation energy to the samples being imaged through the probing laser beam, (ii) undistorted coupling of acoustic waves to the detector without the need for separation of the optical and acoustic paths, (iii) high sensitivity and (iv) ultrawide bandwidth. Because of the unimpeded optical path in PBDT, diffraction-limited lateral resolution can be readily achieved. The sensitivity of the current PBDT sensor of 22 μV/Pa and its noise equivalent pressure (NEP) of 11.4 Pa are comparable with these parameters of the optical micro-ring resonator and commercial piezoelectric ultrasonic transducers. Benefits of the present prototype OA microscope were demonstrated by successfully resolving micron-size details in histological sections of cardiac muscle.

Study of concentration of HPV DNA probe immobilization for cervical cancer detection based IDE biosensor

NASA Astrophysics Data System (ADS)

Roshila, M. L.; Hashim, U.; Azizah, N.

2016-07-01

This paper mainly illustrates regarding the detection process of Human Papillomavirus (HPV) DNA probe. HPV is the most common virus that infected to human by a sexually transmitted virus. The most common high-risk HPV are 16 and 18. Interdigitated electrode (IDE) device used as based of Titanium Dioxide (TiO2) acts as inorganic surface, where by using APTES as a linker between inorganic surface and organic surface. A strategy of rapid and sensitive for the HPV detection was proposed by integrating simple DNA extraction with a gene of DNA. The extraction of the gene of DNA will make an efficiency of the detection process. It will depend on the sequence of the capture probes and the way to support their attached. The fabrication, surface modification, immobilization and hybridization processes are characterized by current voltage (I-V) measurement by using KEITHLEY 6487. This strategy will perform a good sensitivity of HPV detection.

Mode-mismatched confocal thermal-lens microscope with collimated probe beam

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cabrera, Humberto, E-mail: hcabrera@ictp.it; Centro Multidisciplinartio de Ciencias, Instituto Venezolano de Investigaciones Científicas; Korte, Dorota

2015-05-15

We report a thermal lens microscope (TLM) based on an optimized mode-mismatched configuration. It takes advantage of the coaxial counter propagating tightly focused excitation and collimated probe beams, instead of both focused at the sample, as it is in currently known TLM setups. A simple mathematical model that takes into account the main features of the instrument is presented. The confocal detection scheme and the introduction of highly collimated probe beam allow enhancing the versatility, limit of detection (LOD), and sensitivity of the instrument. The theory is experimentally verified measuring ethanol’s absorption coefficient at 532.8 nm. Additionally, the presented techniquemore » is applied for detection of ultra-trace amounts of Cr(III) in liquid solution. The achieved LOD is 1.3 ppb, which represents 20-fold enhancement compared to transmission mode spectrometric techniques and a 7.5-fold improvement compared to previously reported methods for Cr(III) based on thermal lens effect.« less

Design of Selective Substrates and Activity-Based Probes for Hydrolase Important for Pathogenesis 1 (HIP1) from Mycobacterium tuberculosis.

PubMed

Lentz, Christian S; Ordonez, Alvaro A; Kasperkiewicz, Paulina; La Greca, Florencia; O'Donoghue, Anthony J; Schulze, Christopher J; Powers, James C; Craik, Charles S; Drag, Marcin; Jain, Sanjay K; Bogyo, Matthew

2016-11-11

Although serine proteases are important mediators of Mycobacterium tuberculosis (Mtb) virulence, there are currently no tools to selectively block or visualize members of this family of enzymes. Selective reporter substrates or activity-based probes (ABPs) could provide a means to monitor infection and response to therapy using imaging methods. Here, we use a combination of substrate selectivity profiling and focused screening to identify optimized reporter substrates and ABPs for the Mtb "Hydrolase important for pathogenesis 1" (Hip1) serine protease. Hip1 is a cell-envelope-associated enzyme with minimal homology to host proteases, making it an ideal target for probe development. We identified substituted 7-amino-4-chloro-3-(2-bromoethoxy)isocoumarins as irreversible inhibitor scaffolds. Furthermore, we used specificity data to generate selective reporter substrates and to further optimize a selective chloroisocoumarin inhibitor. These new reagents are potentially useful in delineating the roles of Hip1 during pathogenesis or as diagnostic imaging tools for specifically monitoring Mtb infections.

Design of Selective Substrates and Activity-Based Probes for Hydrolase Important for Pathogenesis 1 (HIP1) from Mycobacterium tuberculosis

PubMed Central

2016-01-01

Although serine proteases are important mediators of Mycobacterium tuberculosis (Mtb) virulence, there are currently no tools to selectively block or visualize members of this family of enzymes. Selective reporter substrates or activity-based probes (ABPs) could provide a means to monitor infection and response to therapy using imaging methods. Here, we use a combination of substrate selectivity profiling and focused screening to identify optimized reporter substrates and ABPs for the Mtb “Hydrolase important for pathogenesis 1” (Hip1) serine protease. Hip1 is a cell-envelope-associated enzyme with minimal homology to host proteases, making it an ideal target for probe development. We identified substituted 7-amino-4-chloro-3-(2-bromoethoxy)isocoumarins as irreversible inhibitor scaffolds. Furthermore, we used specificity data to generate selective reporter substrates and to further optimize a selective chloroisocoumarin inhibitor. These new reagents are potentially useful in delineating the roles of Hip1 during pathogenesis or as diagnostic imaging tools for specifically monitoring Mtb infections. PMID:27739665

Can we estimate plasma density in ICP driver through electrical parameters in RF circuit?

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bandyopadhyay, M., E-mail: mainak@iter-india.org; Sudhir, Dass, E-mail: dass.sudhir@iter-india.org; Chakraborty, A., E-mail: arunkc@iter-india.org

2015-04-08

To avoid regular maintenance, invasive plasma diagnostics with probes are not included in the inductively coupled plasma (ICP) based ITER Neutral Beam (NB) source design. Even non-invasive probes like optical emission spectroscopic diagnostics are also not included in the present ITER NB design due to overall system design and interface issues. As a result, negative ion beam current through the extraction system in the ITER NB negative ion source is the only measurement which indicates plasma condition inside the ion source. However, beam current not only depends on the plasma condition near the extraction region but also on the perveancemore » condition of the ion extractor system and negative ion stripping. Nevertheless, inductively coupled plasma production region (RF driver region) is placed at distance (∼ 30cm) from the extraction region. Due to that, some uncertainties are expected to be involved if one tries to link beam current with plasma properties inside the RF driver. Plasma characterization in source RF driver region is utmost necessary to maintain the optimum condition for source operation. In this paper, a method of plasma density estimation is described, based on density dependent plasma load calculation.« less

Micro- and nanodevices integrated with biomolecular probes

PubMed Central

Alapan, Yunus; Icoz, Kutay; Gurkan, Umut A.

2016-01-01

Understanding how biomolecules, proteins and cells interact with their surroundings and other biological entities has become the fundamental design criterion for most biomedical micro- and nanodevices. Advances in biology, medicine, and nanofabrication technologies complement each other and allow us to engineer new tools based on biomolecules utilized as probes. Engineered micro/nanosystems and biomolecules in nature have remarkably robust compatibility in terms of function, size, and physical properties. This article presents the state of the art in micro- and nanoscale devices designed and fabricated with biomolecular probes as their vital constituents. General design and fabrication concepts are presented and three major platform technologies are highlighted: microcantilevers, micro/nanopillars, and microfluidics. Overview of each technology, typical fabrication details, and application areas are presented by emphasizing significant achievements, current challenges, and future opportunities. PMID:26363089

A Water-Soluble, Two-Photon Probe for Imaging Endogenous Hypochlorous Acid in Live Tissue.

PubMed

Xing, Panfei; Feng, Yanxian; Niu, Yiming; Li, Qiu; Zhang, Zhe; Dong, Lei; Wang, Chunming

2018-04-17

Detection of hypochlorous acid (HClO) in the living system may help to uncover its essential biological functions. However, current imaging agents suffer from poor water solubility that limit their live-tissue applications. Here, a water-soluble probe (NNH) is devised through innovative hydrazone modification of 1,8-naphthalimide at 3' position. NNH was successfully applied to tracking endogenous HClO in both cultured macrophages and a liver injury model in mice. NNH demonstrated remarkably increased water solubility and multiple desirable features including two-photon absorbance, anti-bleaching capability, rapid cellular uptake, and low cytotoxicity. NNH is the first hydrazone-based probe for real-time imaging of HClO in live tissue. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

A Simple Method for Amplifying RNA Targets (SMART)

PubMed Central

McCalla, Stephanie E.; Ong, Carmichael; Sarma, Aartik; Opal, Steven M.; Artenstein, Andrew W.; Tripathi, Anubhav

2012-01-01

We present a novel and simple method for amplifying RNA targets (named by its acronym, SMART), and for detection, using engineered amplification probes that overcome existing limitations of current RNA-based technologies. This system amplifies and detects optimal engineered ssDNA probes that hybridize to target RNA. The amplifiable probe-target RNA complex is captured on magnetic beads using a sequence-specific capture probe and is separated from unbound probe using a novel microfluidic technique. Hybridization sequences are not constrained as they are in conventional target-amplification reactions such as nucleic acid sequence amplification (NASBA). Our engineered ssDNA probe was amplified both off-chip and in a microchip reservoir at the end of the separation microchannel using isothermal NASBA. Optimal solution conditions for ssDNA amplification were investigated. Although KCl and MgCl2 are typically found in NASBA reactions, replacing 70 mmol/L of the 82 mmol/L total chloride ions with acetate resulted in optimal reaction conditions, particularly for low but clinically relevant probe concentrations (≤100 fmol/L). With the optimal probe design and solution conditions, we also successfully removed the initial heating step of NASBA, thus achieving a true isothermal reaction. The SMART assay using a synthetic model influenza DNA target sequence served as a fundamental demonstration of the efficacy of the capture and microfluidic separation system, thus bridging our system to a clinically relevant detection problem. PMID:22691910

Handheld scanning probes for optical coherence tomography: developments, applications, and perspectives

NASA Astrophysics Data System (ADS)

Duma, V.-F.; Demian, D.; Sinescu, C.; Cernat, R.; Dobre, G.; Negrutiu, M. L.; Topala, F. I.; Hutiu, Gh.; Bradu, A.; Podoleanu, A. G.

2016-03-01

We present the handheld scanning probes that we have recently developed in our current project for biomedical imaging in general and for Optical Coherence Tomography (OCT) in particular. OCT is an established, but dynamic imagistic technique based on laser interferometry, which offers micrometer resolutions and millimeters penetration depths. With regard to existing devices, the newly developed handheld probes are simple, light and relatively low cost. Their design is described in detail to allow for the reproduction in any lab, including for educational purposes. Two probes are constructed almost entirely from off-the-shelf components, while a third, final variant is constructed with dedicated components, in an ergonomic design. The handheld probes have uni-dimensional (1D) galvanometer scanners therefore they achieve transversal sections through the biological sample investigated - in contrast to handheld probes equipped with bi-dimensional (2D) scanners that can also achieve volumetric (3D) reconstructions of the samples. These latter handheld probes are therefore also discussed, as well as the possibility to equip them with galvanometer 2D scanners or with Risley prisms. For galvanometer scanners the optimal scanning functions studied in a series of previous works are pointed out; these functions offer a higher temporal efficiency/duty cycle of the scanning process, as well as artifact-free OCT images. The testing of the handheld scanning probes in dental applications is presented, for metal ceramic prosthesis and for teeth.

Eddy Current Probe for Surface and Sub-Surface Inspection

NASA Technical Reports Server (NTRS)

Wincheski, Russell A. (Inventor); Simpson, John W. (Inventor)

2014-01-01

An eddy current probe includes an excitation coil for coupling to a low-frequency alternating current (AC) source. A magneto-resistive sensor is centrally disposed within and at one end of the excitation coil to thereby define a sensing end of the probe. A tubular flux-focusing lens is disposed between the excitation coil and the magneto-resistive sensor. An excitation wire is spaced apart from the magneto-resistive sensor in a plane that is perpendicular to the sensor's axis of sensitivity and such that, when the sensing end of the eddy current probe is positioned adjacent to the surface of a structure, the excitation wire is disposed between the magneto-resistive sensor and the surface of the structure. The excitation wire is coupled to a high-frequency AC source. The excitation coil and flux-focusing lens can be omitted when only surface inspection is required.

GMR-based eddy current probe for weld seam inspection and its non-scanning detection study

NASA Astrophysics Data System (ADS)

Gao, Peng; Wang, Chao; Li, Yang; Wang, Libin; Cong, Zheng; Zhi, Ya

2017-04-01

Eddy current testing is one of the most important non-destructive testing methods for welding defects detection. This paper presents the use of a probe consisting of 4 giant magneto-resistive (GMR) sensors to detect weld defects. Information from four measuring points above and on both sides of the weld seam is collected at the same time. By setting the GMR sensors' sensing axes perpendicular to the direction of the excitation magnetic field, the information collected mainly reflects the change in the eddy current which is caused by defects. Digital demodulation technology is applied to extract the real part and imaginary part of the GMR sensors' output signals. The variables containing directional information of the magnetic field are introduced. Based on the data from the four GMR (4-GMR) sensors' output signals, four values, Ran, Mean, Var and k are selected as the feature quantities for defect recognition. Experiments are carried out on weld seams with and without defects, and the detection outputs are given in this paper. The 4-GMR probe is also employed to investigate non-scanning weld defect detection and the four feature quantities (Ran, Mean, Var and k) are studied to evaluate weld quality. The non-scanning weld defect detection is presented. A support vector machine is used to classify and discriminate welds with and without defects. Experiments carried out show that through the method in this paper, the recognition rate is 92% for welds without defects and 90% for welds with defects, with an overall recognition rate of 90.9%, indicating that this method could effectively detect weld defects.

Probing ultra-fast processes with high dynamic range at 4th-generation light sources: Arrival time and intensity binning at unprecedented repetition rates.

PubMed

Kovalev, S; Green, B; Golz, T; Maehrlein, S; Stojanovic, N; Fisher, A S; Kampfrath, T; Gensch, M

2017-03-01

Understanding dynamics on ultrafast timescales enables unique and new insights into important processes in the materials and life sciences. In this respect, the fundamental pump-probe approach based on ultra-short photon pulses aims at the creation of stroboscopic movies. Performing such experiments at one of the many recently established accelerator-based 4th-generation light sources such as free-electron lasers or superradiant THz sources allows an enormous widening of the accessible parameter space for the excitation and/or probing light pulses. Compared to table-top devices, critical issues of this type of experiment are fluctuations of the timing between the accelerator and external laser systems and intensity instabilities of the accelerator-based photon sources. Existing solutions have so far been only demonstrated at low repetition rates and/or achieved a limited dynamic range in comparison to table-top experiments, while the 4th generation of accelerator-based light sources is based on superconducting radio-frequency technology, which enables operation at MHz or even GHz repetition rates. In this article, we present the successful demonstration of ultra-fast accelerator-laser pump-probe experiments performed at an unprecedentedly high repetition rate in the few-hundred-kHz regime and with a currently achievable optimal time resolution of 13 fs (rms). Our scheme, based on the pulse-resolved detection of multiple beam parameters relevant for the experiment, allows us to achieve an excellent sensitivity in real-world ultra-fast experiments, as demonstrated for the example of THz-field-driven coherent spin precession.

Miniature and Molecularly Specific Optical Screening Technologies for Breast Cancer

DTIC Science & Technology

2008-10-01

commercially available dual-channel transimpedance amplifier circuit boards (Boston Electronics, TWAMP). Preliminary results with the imaging probe...connected to a current amplifier via a coaxial cable for diffuse reflectance measurements. This new probe is named P4-3 and schematics of the system and...probe. With the single pixel device a single-channel current amplifier (Terahertz Technologies, PDA-750) could easily read and collect the photocurrent

Electronegative plasma diagnostic by laser photo-detachment combined with negatively biased Langmuir probe

NASA Astrophysics Data System (ADS)

Oudini, N.; Sirse, N.; Taccogna, F.; Ellingboe, A. R.; Bendib, A.

2018-05-01

We propose a new technique for diagnosing negative ion properties using Langmuir probe assisted pulsed laser photo-detachment. While the classical technique uses a laser pulse to convert negative ions into electron-atom pairs and a positively biased Langmuir probe tracking the change of electron saturation current, the proposed method uses a negatively biased Langmuir probe to track the temporal evolution of positive ion current. The negative bias aims to avoid the parasitic electron current inherent to probe tip surface ablation. In this work, we show through analytical and numerical approaches that, by knowing electron temperature and performing photo-detachment at two different laser wavelengths, it is possible to deduce plasma electronegativity (ratio of negative ion to electron densities) α, and anisothermicity (ratio of electron to negative ion temperatures) γ-. We present an analytical model that links the change in the collected positive ion current to plasma electronegativity and anisothermicity. Particle-In-Cell simulation is used as a numerical experiment covering a wide range of α and γ- to test the new analysis technique. The new technique is sensitive to α in the range 0.5 < α < 10 and yields γ- for large α, where negative ion flux affects the probe sheath behavior, typically α > 1.

Nonequilibrium dynamics of probe filaments in actin-myosin networks

NASA Astrophysics Data System (ADS)

Gladrow, J.; Broedersz, C. P.; Schmidt, C. F.

2017-08-01

Active dynamic processes of cells are largely driven by the cytoskeleton, a complex and adaptable semiflexible polymer network, motorized by mechanoenzymes. Small dimensions, confined geometries, and hierarchical structures make it challenging to probe dynamics and mechanical response of such networks. Embedded semiflexible probe polymers can serve as nonperturbing multiscale probes to detect force distributions in active polymer networks. We show here that motor-induced forces transmitted to the probe polymers are reflected in nonequilibrium bending dynamics, which we analyze in terms of spatial eigenmodes of an elastic beam under steady-state conditions. We demonstrate how these active forces induce correlations among the mode amplitudes, which furthermore break time-reversal symmetry. This leads to a breaking of detailed balance in this mode space. We derive analytical predictions for the magnitude of resulting probability currents in mode space in the white-noise limit of motor activity. We relate the structure of these currents to the spatial profile of motor-induced forces along the probe polymers and provide a general relation for observable currents on two-dimensional hyperplanes.

Profiling of barrier capacitance and spreading resistance using a transient linearly increasing voltage technique.

PubMed

Gaubas, E; Ceponis, T; Kusakovskij, J

2011-08-01

A technique for the combined measurement of barrier capacitance and spreading resistance profiles using a linearly increasing voltage pulse is presented. The technique is based on the measurement and analysis of current transients, due to the barrier and diffusion capacitance, and the spreading resistance, between a needle probe and sample. To control the impact of deep traps in the barrier capacitance, a steady state bias illumination with infrared light was employed. Measurements of the spreading resistance and barrier capacitance profiles using a stepwise positioned probe on cross sectioned silicon pin diodes and pnp structures are presented.

Temperature measurement based on photoluminescence of Er3+ doped Sr0.3Cd0.7F2 microcrystal coupled to scanning thermal microscopy

NASA Astrophysics Data System (ADS)

Trannoy, N.; Sayoud, A.; Diaf, M.; Duvaut, Th.; Jouart, J. P.; Grossel, Ph.

2015-04-01

Rare earth doped sub-micrometric luminescent materials are promising candidates for temperature sensing and play an efficient role in many technological fields. In this paper, a new optical sensor is developed for measuring local temperatures. This sensor is based on a thermal-resistive probe and on photoluminescence of a luminescent fluoride microcrystal. The final purpose is to develop a device calibrated in temperature and capable of acquiring images of local temperature at sub-micrometric scale. Indeed, the sensor temperature can be obtained in two distinct ways: one from the thermal probe parameters and the other from the green photoluminescence generated in the anti-Stokes mode by the active Er ions directly excited by a red laser. The thermal probe is based on Wollaston wire whose thermal-resistive element is in platinum/rhodium. Its temperature is estimated from the probe electrical characteristics and a modeling. A microcrystal of Sr0.3Cd0.7F2: Er3+(4%)-Yb3+(6%) of about 25 μm in diameter is glued at the probe extremity. This luminescent material has the particularity to give a green emission spectrum with intensities sensitive to small temperature variations. Using the fluorescence intensity ratio (FIR) technique, the crystal temperature is estimated from the intensity measurements at green wavelengths 522, 540 and 549 nm by taking advantage of particular optical properties due to the crystalline nature of Sr0.3Cd0.7F2: Er3+-Yb3+. The microcrystal temperature is then assessed as a function of electric current in the thermal probe by applying the Boltzmann's equations. The coupling of the scanning thermal microscope (SThM) with the photoluminescence probe reveals that the particle fluorescence signal is affected by the temperature rise of an electrical microsystem submitted to a Joule heating. The first results are presented and discussed.

Learning Probe: Benchmarking for Excellence. Questionnaire. Second Edition.

ERIC Educational Resources Information Center

Owen, Jane; Yarrow, David; Appleby, Alex

This document is a questionnaire designed for work-based learning providers. It is a diagnostic benchmarking tool developed to give organizations a snapshot of their current state. Following a brief introduction, there are instructions for filling in the questionnaire, which includes both open-ended response and scoring according to a…

Quantitative imaging of glutathione in live cells using a reversible reaction-based ratiometric fluorescent probe

USDA-ARS?s Scientific Manuscript database

Glutathione (GSH) plays an important role in maintaining redox homeostasis inside cells. Currently, there are no methods available to quantitatively assess the GSH concentration in live cells. Live cell fluorescence imaging revolutionized the understanding of cell biology and has become an indispens...

QUANTITATIVE MEASUREMENT OF STACHYBOTRYS CHARTARUM CONIDIA USING REAL TIME DETECTION OF PCR PRODUCTS WITH THE TAQMAN TM FLUOROGENIC PROBE SYSTEM

EPA Science Inventory

The occurence of Stachybotrys chartarum in indoor environments has been associated with a number of human health concerns, including fatal pulmonary haemosiderosis in infants. Currently used culture-based and microscopic methods of fungal species identification are poorly suited ...

To probe the equivalence and opulence of nanocrystal and nanotube based dye-sensitized solar cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jyoti, Divya, E-mail: divyabathla17@gmail.com; Mohan, Devendra

2016-05-06

Dye-Sensitized solar cells based on TiO{sub 2} nanocrystal and TiO{sub 2} nanotubes have been fabricated by a simple sol-gel hydrothermal process and their performances have been compared. Current density and voltage (JV) characteristics and incident photon to current conversion efficiency (IPCE) plots have been set as criterion to check which one is better as a photoanode candidate in dye-sensitized solar cell. It has been observed that although open circuit voltage values for both type of cells do not differ much still, nanotube based dye-sensitized solar cells are more successful having an efficiency value of 7.28%.

Open carbon nanopipettes as resistive-pulse sensors, rectification sensors, and electrochemical nanoprobes.

PubMed

Hu, Keke; Wang, Yixian; Cai, Huijing; Mirkin, Michael V; Gao, Yang; Friedman, Gary; Gogotsi, Yury

2014-09-16

Nanometer-sized glass and quartz pipettes have been widely used as a core of chemical sensors, patch clamps, and scanning probe microscope tips. Many of those applications require the control of the surface charge and chemical state of the inner pipette wall. Both objectives can be attained by coating the inner wall of a quartz pipette with a nanometer-thick layer of carbon. In this letter, we demonstrate the possibility of using open carbon nanopipettes (CNP) produced by chemical vapor deposition as resistive-pulse sensors, rectification sensors, and electrochemical nanoprobes. By applying a potential to the carbon layer, one can change the surface charge and electrical double-layer at the pipette wall, which, in turn, affect the ion current rectification and adsorption/desorption processes essential for resistive-pulse sensors. CNPs can also be used as versatile electrochemical probes such as asymmetric bipolar nanoelectrodes and dual electrodes based on simultaneous recording of the ion current through the pipette and the current produced by oxidation/reduction of molecules at the carbon nanoring.

Developing and testing a multi-probe resonance electrical impedance spectroscopy system for detecting breast abnormalities

NASA Astrophysics Data System (ADS)

Gur, David; Zheng, Bin; Dhurjaty, Sreeram; Wolfe, Gene; Fradin, Mary; Weil, Richard; Sumkin, Jules; Zuley, Margarita

2009-02-01

In our previous study, we reported on the development and preliminary testing of a prototype resonance electrical impedance spectroscopy (REIS) system with a pair of probes. Although our pilot study on 150 young women ranging from 30 to 50 years old indicated the feasibility of using REIS output sweep signals to classify between the women who had negative examinations and those who would ultimately be recommended for biopsy, the detection sensitivity was relatively low. To improve performance when using REIS technology, we recently developed a new multi-probe based REIS system. The system consists of a sensor module box that can be easily lifted along a vertical support device to fit women of different height. Two user selectable breast placement "cups" with different curvatures are included in the system. Seven probes are mounted on each of the cups on opposing sides of the sensor box. By rotating the sensor box, the technologist can select the detection sensor cup that better fits the breast size of the woman being examined. One probe is mounted in the cup center for direct contact with the nipple and the other six probes are uniformly distributed along an outside circle to enable contact with six points on the outer and inner breast skin surfaces. The outer probes are located at a distance of 60mm away from the center (nipple) probe. The system automatically monitors the quality of the contact between the breast surface and each of the seven probes and data acquisition can only be initiated when adequate contact is confirmed. The measurement time for each breast is approximately 15 seconds during which time the system records 121 REIS signal sweep outputs generated from 200 KHz to 800 KHz at 5 KHz increments for all preselected probe pairs. Currently we are measuring 6 pairs between the center probe and each of six probes located on the outer circle as well as two pairs between probe pairs on the outer circle. This new REIS system has been installed in our clinical breast imaging facility. We are conducting a prospective study to assess performance when using this REIS system under an approved IRB protocol. Over 200 examinations have been conducted to date. Our experience showed that this new REIS system was easy to operate and the REIS examination was fast and considered "comfortable" by examinees since the women presses her breast into the cup herself without any need for forced breast compression, and all but a few highly sensitive women have any sensation of an electrical current during the measurement.

Magnetically Filtered Faraday Probe for Measuring the Ion Current Density Profile of a Hall Thruster

DTIC Science & Technology

2006-01-01

Hall thruster is investigated. The MFFP is designed to eliminate the collection of low-energy, charge-exchange (CEX) ions by using a variable magnetic field as an ion filter. In this study, a MFFP, Faraday probe with a reduced acceptance angle (BFP), and nude Faraday probe are used to measure the ion current density profile of a 5 kW Hall thruster operating over the range of 300-500 V and 5-10 mg/s. The probes are evaluated on a xenon propellant Hall thruster in the University of Michigan Large Vacuum Test Facility at operating

Methods of and apparatus for levitating an eddy current probe

DOEpatents

Stone, William J.

1988-05-03

An eddy current probe is supported against the force of gravity with an air earing while being urged horizontally toward the specimen being examined by a spring and displaced horizontally against the force of the spring pneumatically. The pneumatic displacement is accomplished by flowing air between a plenum chamber fixed with respect to the probe and the surface of the specimen. In this way, the surface of the specimen can be examined without making mechanical contact therewith while precisely controlling the distance at which the probe stands-off from the surface of the specimen.

Operation of a swept Langmuir probe on a sounding rocket

NASA Astrophysics Data System (ADS)

Robertson, S. H.; Dickson, S.; Friedrich, M.; Sternovsky, Z.

2012-12-01

A swept cylindrical Langmuir probe was operated on two sounding rockets from ~ 60-120 km for the purpose of determining both the ambient electron density and the payload potential relative to the ambient plasma. The rockets were part of the CHAMPS (CHarge And mass of Meteoritic smoke ParticleS) rocket campaign and carried mass analyzers and various plasma probes to study charged meteoritic dust in the mesopause region. The payload potential is an important parameter for data interpretation. The rockets were launched in October of 2011 from Andøya Rocket Range, Norway. The launches were a few days apart with one taking place during the day and the other at night. The swept Langmuir probe data provided a current-voltage characteristic that had a distinct "knee" indicating the onset of electron collection; the probe voltage at this "knee" corresponds to the ambient plasma potential. The data indicate a payload potential of about -2 V to -1 V for both launches. The payload potential becomes less negative for altitudes above 80 km on the day launch due to photoemission. The probe current-voltage data are also compared with ion and electron density measurements from ion probes and Faraday rotation antennas, respectively. The data from the various instruments are in general agreement. Further consideration of the Langmuir probe performance shows that if the probe had been operated with feedback control to continuously collect electrons with a current of order 1 microamp, the probe potential would be an accurate, continuous indicator of the payload potential without the need for sweeping which could periodically alter the payload potential.

Capacitively coupled RF voltage probe having optimized flux linkage

DOEpatents

Moore, James A.; Sparks, Dennis O.

1999-02-02

An RF sensor having a novel current sensing probe and a voltage sensing probe to measure voltage and current. The current sensor is disposed in a transmission line to link all of the flux generated by the flowing current in order to obtain an accurate measurement. The voltage sensor is a flat plate which operates as a capacitive plate to sense voltage on a center conductor of the transmission line, in which the measured voltage is obtained across a resistance leg of a R-C differentiator circuit formed by the characteristic impedance of a connecting transmission line and a capacitance of the plate, which is positioned proximal to the center conductor.

Project Icarus: Preliminary Thoughts on the Selection of Probes and Instruments for an Icarus-style Interstellar Mission

NASA Astrophysics Data System (ADS)

Crawford, Ian A.

2016-06-01

In this paper we outline the range of probes and scientific instruments that will be required in order for Icarus to fulfill its scientific mission of exploring a nearby star, its attendant planetary system, and the intervening interstellar medium. Based on this preliminary analysis, we estimate that the minimum total Icarus scientific payload mass (i.e. the mass of probes and instruments which must be decelerated to rest in the target system to enable a meaningful programme of scientific investigation) will be in the region of 100 tonnes. Of this, approximately 10 tonnes would be allocated for cruise-phase science instruments, and about 35 tonnes (i.e. the average of estimated lower and upper limits of 28 and 41 tonnes) would be contributed by the intra-system science payload itself (i.e. the dry mass of the stellar and planetary probes and their instruments). The remaining ~55 tonnes is allocated for the sub-probe intra-system propulsion requirements (crudely estimated from current Solar System missions; detailed modelling of sub-probe propulsion systems will be needed to refine this figure). The overall mass contributed by the science payload to the total that must be decelerated from the interstellar cruise velocity will be considerably more than 100 tonnes, however, as allowance must be made for the payload structural and infrastructural elements required to support, deploy, and communicate with the science probes and instruments. Based on the earlier Daedalus study, we estimate another factor of two to allow for these components. Pending the outcome of more detailed studies, it therefore appears that an overall science-related payload mass of ~200 tonnes will be required. This paper is a submission of the Project Icarus Study Group.

Automated measurement system employing eddy currents to adjust probe position and determine metal hardness

DOEpatents

Prince, J.M.; Dodson, M.G.; Lechelt, W.M.

1989-07-18

A system for measuring the hardness of cartridge cases employs an eddy current probe for inducing and sensing eddy currents in each cartridge case. A first component of the sensed signal is utilized in a closed loop system for accurately positioning the probe relative to the cartridge case both in the lift off direction and in the tangential direction, and a second component of the sensed signal is employed as a measure of the hardness. The positioning and measurement are carried out under closed loop microprocessor control facilitating hardness testing on a production line basis. 14 figs.

Automated measurement system employing eddy currents to adjust probe position and determine metal hardness

DOEpatents

Prince, James M.; Dodson, Michael G.; Lechelt, Wayne M.

1989-01-01

A system for measuring the hardness of cartridge cases employs an eddy current probe for inducing and sensing eddy currents in each cartridge case. A first component of the sensed signal is utilized in a closed loop system for accurately positioning the probe relative to the cartridge case both in the lift off direction and in the tangential direction, and a second component of the sensed signal is employed as a measure of the hardness. The positioning and measurement are carried out under closed loop microprocessor control facilitating hardness testing on a production line basis.

The Space High Angular Resolution Probe for the Infrared (SHARP-IR)

NASA Technical Reports Server (NTRS)

Rinehart, S. A.; Rizzo, M. J.; Leisawitz, D. T.; Staguhn, J. G.; Dipirro, M.; Mentzell, J. E.; Juanola-Parramon, R.; Dhabal, A.; Mundy, L. G.; Moseley, S. H.;

Electromechanical Apparatus Measures Residual Stress

NASA Technical Reports Server (NTRS)

Chern, Engmin J.; Flom, Yury

1993-01-01

Nondestructive test exploits relationship between stress and eddy-current-probe resistance. Yields data on residual stress or strain in metal tension/compression specimen (stress or strain remaining in specimen when no stress applied from without). Apparatus is assembly of commercial equipment: tension-or-compression testing machine, eddy-current probe, impedance gain-and-phase analyzer measuring impedance of probe coil, and desktop computer, which controls other equipment and processes data received from impedance gain-and-phase analyzer.

Feasibility of a GNSS-Probe for Creating Digital Maps of High Accuracy and Integrity

NASA Astrophysics Data System (ADS)

Vartziotis, Dimitris; Poulis, Alkis; Minogiannis, Alexandros; Siozos, Panayiotis; Goudas, Iraklis; Samson, Jaron; Tossaint, Michel

The “ROADSCANNER” project addresses the need for increased accuracy and integrity Digital Maps (DM) utilizing the latest developments in GNSS, in order to provide the required datasets for novel applications, such as navigation based Safety Applications, Advanced Driver Assistance Systems (ADAS) and Digital Automotive Simulations. The activity covered in the current paper is the feasibility study, preliminary tests, initial product design and development plan for an EGNOS enabled vehicle probe. The vehicle probe will be used for generating high accuracy, high integrity and ADAS compatible digital maps of roads, employing a multiple passes methodology supported by sophisticated refinement algorithms. Furthermore, the vehicle probe will be equipped with pavement scanning and other data fusion equipment, in order to produce 3D road surface models compatible with standards of road-tire simulation applications. The project was assigned to NIKI Ltd under the 1st Call for Ideas in the frame of the ESA - Greece Task Force.

Probes of Ubiquitin E3 ligases distinguish different stages of Parkin activation

PubMed Central

Pao, Kuan-Chuan; Stanley, Mathew; Han, Cong; Lai, Yu-Chiang; Murphy, Paul; Balk, Kristin; Wood, Nicola T.; Corti, Olga; Corvol, Jean-Christophe; Muqit, Miratul M.K.; Virdee, Satpal

2016-01-01

E3 ligases represent an important class of enzymes, yet there are currently no chemical probes to profile their activity. We develop a new class of activity-based probe by reengineering of a ubiquitin-charged E2 conjugating enzyme and demonstrate their utility by profiling the transthiolation activity of the RING-in-between-RING (RBR) E3 ligase Parkin in vitro and in cellular extracts. Our study provides valuable insight into the roles, and cellular hierarchy, of distinct phosphorylation events in Parkin activation. We also profile Parkin patient disease-associated mutations and strikingly demonstrate that they largely mediate their effect by altering transthiolation activity. Furthermore, our probes enable direct and quantitative measurement of endogenous Parkin activity revealing that endogenous Parkin is activated in neuronal cell lines (≥75 %) in response to mitochondrial depolarization. This new technology also holds promise as a novel biomarker of PINK1-Parkin signalling as demonstrated by compatibility with Parkinson’s disease patient-derived samples. PMID:26928937

Optical probe for porosity defect detection on inner diameter surfaces of machined bores

NASA Astrophysics Data System (ADS)

Kulkarni, Ojas P.; Islam, Mohammed N.; Terry, Fred L.

2010-12-01

We demonstrate an optical probe for detection of porosity inside spool bores of a transmission valve body with diameters down to 5 mm. The probe consists of a graded-index relay rod that focuses a laser beam spot onto the inner surface of the bore. Detectors, placed in the specular and grazing directions with respect to the incident beam, measure the change in scattered intensity when a surface defect is encountered. Based on the scattering signatures in the two directions, the system can also validate the depth of the defect and distinguish porosity from bump-type defects coming out of the metal surface. The system can detect porosity down to a 50-μm lateral dimension and ~40 μm in depth with >3-dB contrast over the background intensity fluctuations. Porosity detection systems currently use manual inspection techniques on the plant floor, and the demonstrated probe provides a noncontact technique that can help automotive manufacturers meet high-quality standards during production.

Differential geometry based model for eddy current inspection of U-bend sections in steam generator tubes

NASA Astrophysics Data System (ADS)

Mukherjee, Saptarshi; Rosell, Anders; Udpa, Lalita; Udpa, Satish; Tamburrino, Antonello

2017-02-01

The modeling of U-Bend segment in steam generator tubes for predicting eddy current probe signals from cracks, wear and pitting in this region poses challenges and is non-trivial. Meshing the geometry in the cartesian coordinate system might require a large number of elements to model the U-bend region. Also, since the lift-off distance between the probe and tube wall is usually very small, a very fine mesh is required near the probe region to accurately describe the eddy current field. This paper presents a U-bend model using differential geometry principles that exploit the result that Maxwell's equations are covariant with respect to changes of coordinates and independent of metrics. The equations remain unaltered in their form, regardless of the choice of the coordinates system, provided the field quantities are represented in the proper covariant and contravariant form. The complex shapes are mapped into simple straight sections, while small lift-off is mapped to larger values, thus reducing the intrinsic dimension of the mesh and stiffness matrix. In this contribution, the numerical implementation of the above approach will be discussed with regard to field and current distributions within the U-bend tube wall. For the sake of simplicity, a two dimensional test case will be considered. The approach is evaluated in terms of efficiency and accuracy by comparing the results with that obtained using a conventional FE model in cartesian coordinates.

The Fixed-Bias Langmuir Probe on the Communication-Navigation Outage Forecast System Satellite: Calibration and Validation

NASA Technical Reports Server (NTRS)

Klenzing, J.; Rowland, D.

2012-01-01

A fixed-bias spherical Langmuir probe is included as part of the Vector Electric Field Instrument (VEFI) suite on the Communication Navigation Outage Forecast System (CNOFS) satellite.CNOFS gathers data in the equatorial ionosphere between 400 and 860 km, where the primary constituent ions are H+ and O+. The ion current collected by the probe surface per unit plasma density is found to be a strong function of ion composition. The calibration of the collected current to an absolute density is discussed, and the performance of the spherical probe is compared to other in situ instruments on board the CNOFS satellite. The application of the calibration is discussed with respect to future fixed-bias probes; in particular, it is demonstrated that some density fluctuations will be suppressed in the collected current if the plasma composition rapidly changes along with density. This is illustrated in the observation of plasma density enhancements on CNOFS.

Empirical Modeling of the Storm Time Innermost Magnetosphere Using Van Allen Probes and THEMIS Data: Eastward and Banana Currents

NASA Technical Reports Server (NTRS)

Stephens, G. K.; Sitnov, M. I.; Ukhorskiy, A. Y.; Roelof, E. C.; Tsyganenko, N. A.; Le, G.

2016-01-01

The structure of storm time currents in the inner magnetosphere, including its innermost region inside 4R(sub E), is studied for the first time using a modification of the empirical geomagnetic field model TS07D and new data from Van Allen Probes and Time History of Events and Macroscale Interactions during Substorms missions. It is shown that the model, which uses basis-function expansions instead of ad hoc current modules to approximate the magnetic field, consistently improves its resolution and magnetic field reconstruction with the increase of the number of basis functions and resolves the spatial structure and evolution of the innermost eastward current. This includes a connection between the westward ring current flowing largely at R > or approx. 3R(sub E) and the eastward ring current concentrated at R < or approx. 3R(sub E) resulting in a vortex current pattern. A similar pattern coined 'banana current' was previously inferred from the pressure distributions based on the energetic neutral atom imaging and first-principles ring current simulations. The morphology of the equatorial currents is dependent on storm phase. During the main phase, it is complex, with several asymmetries forming banana currents. Near SYM-H minimum, the banana current is strongest, is localized in the evening-midnight sector, and is more structured compared to the main phase. It then weakens during the recovery phase resulting in the equatorial currents to become mostly azimuthally symmetric.

A wide linear range Eddy Current Displacement Sensor equipped with dual-coil probe applied in the Magnetic Suspension Flywheel.

PubMed

Fang, Jiancheng; Wen, Tong

2012-01-01

The Eddy Current Displacement Sensor (ECDS) is widely used in the Magnetic Suspension Flywheel (MSFW) to measure the tiny clearance between the rotor and the magnetic bearings. The linear range of the ECDS is determined by the diameter of its probe coil. Wide clearances must be measured in some new MSFWs recently designed for the different space missions, but the coil diameter is limited by some restrictions. In this paper, a multi-channel ECDS equipped with dual-coil probes is proposed to extend the linear range to satisfy the demands of such MSFWs. In order to determine the best configuration of the dual-coil probe, the quality factors of the potential types of the dual-coil probes, the induced eddy current and the magnetic intensity on the surface of the measuring object are compared with those of the conventional single-coil probe. The linear range of the ECDS equipped with the selected dual-coil probe is extended from 1.1 mm to 2.4 mm under the restrictions without adding any cost for additional compensation circuits or expensive coil materials. The effectiveness of the linear range extension ability and the dynamic response of the designed ECDS are confirmed by the testing and the applications in the MSFW.

Micromachined Silicon Stimulating Probes with CMOS Circuitry for Use in the Central Nervous System

NASA Astrophysics Data System (ADS)

Tanghe, Steven John

1992-01-01

Electrical stimulation in the central nervous system is a valuable technique for studying neural systems and is a key element in the development of prostheses for deafness and other disorders. This thesis presents a family of multielectrode probe structures, fulfilling the need for chronic multipoint stimulation tools essential for interfacing to the highly complex neural networks in the brain. These probes are batch-fabricated on silicon wafers, employing photoengraving techniques to precisely control the electrode site and array geometries and to allow the integration of on-chip CMOS circuitry for signal multiplexing and stimulus current generation. Silicon micromachining is used to define the probe shapes, which have typical shank dimensions of 3 mm in length by 100 mu m in width by 15 μm in thickness. Each shank supports up to eight planar iridium oxide electrode sites capable of delivering charge densities in excess of 3 mC/cm^2 during current pulse stimulation. Three active probe circuits have been designed with varied complexity and capability. All three can deliver biphasic stimulus currents through 16 sites using only 5 external leads, and they are all compatible with the same external control system. The most complex design interprets site addresses and stimulus current amplitudes from 16-bit words shifted into the probe at 4 MHz. Sixteen on-chip, biphasic, 8-bit digital-to-analog converters deliver analog stimulus currents in the range of +/- 254 muA to any combination of electrode sites. These DACs exhibit full-scale internal linearity to better than +/-1/2 LSB and can be calibrated by varying the positive power supply voltage. The entire probe circuit dissipates only 80 muW from +/-5 V supplies when not delivering stimulus currents, it includes several safety features, and is testable from the input pads. Test results from the fabricated circuits indicate that they all function properly at clocking frequencies as high as 10 MHz, meeting or exceeding all design specifications. Probe structures without circuitry have been used for stimulation experiments in guinea pigs yielding excellent results.

Some experiments with the tunnel probe in a low temperature magnetized plasma

NASA Astrophysics Data System (ADS)

Kovačič, J.; Gyergyek, T.; Kavaš, B.; Vodnik, M.; Kavčič, J.; Gunn, J. P.

2018-02-01

Experiments were performed using a Tunnel Probe (TP) inside the weakly-ionised plasma of the Linear Magnetized Plasma Device (LMPD). The TP is designed as a concave probe, which should annihilate the problem of sheath expansion in the ion branch of the I-V characteristic. As the ion saturation current is consequently well defined, the ion parallel current and plasma density can be more accurately calculated. Furthermore the ratio between the ion saturation currents on the two collectors (tunnel ring and the back-plate) can be used to derive the electron temperature. The TP has been repeatedly used with success on the former Castor and Tore-Supra tokamaks and will be used on the upgraded version of Tore-supra, namely the WEST tokamak, as well [1, 2]. It was however never used successfully in a low-temperature plasma. We studied the feasibility of the TP use in a low-temperature plasma for direct measurements of plasma temperature and density. The various probe characteristic dimensions, such as the distance between the two collectors, the aperture size and the probe radius were varied to see influence of the individual probe feature. We also varied the level of magnetization of the charged particle species, the background gas pressure (which influences the electron energy distribution function), the plasma density (important for the ratio between the λ D and the ion Larmor radius). The sensitivity of the probe alignment to the magnetic field lines was also studied. We found, that the ion saturation current does not necessarily saturate and that the probe works according to expectations only in a limited amount of regimes.

Characteristics of Ferromagnetic Flux Focusing Lens in the Development of Surface/Subsurface Flaw Detector

NASA Technical Reports Server (NTRS)

Wincheski, Buzz; Fulton, Jim; Nath, Shridhar; Namkung, Min; Simpson, John

1993-01-01

Electromagnetic NDE techniques have in the past steered away from the use of ferromagnetic materials. Although their high permeabilities lead to increased field levels, the properties of ferrous elements in the presence of alternating magnetic fields are difficult to determine. In addition, their use leads to losses which can be minimized through the use of low conductivity ferrites. In fact, the eddy current probes which do incorporate ferromagnetic materials have focused on these losses and the shielding which can be obtained by surrounding a probe with a high permeability, conducting material. Eddy current probes enclosed in conducting and magnetic shields have been used to prevent the generated fields from interacting with materials in the vicinity of the probe, such as when testing near material boundaries. A recent invention has used ferromagnetic shielding to magnetically separate individual concentric eddy current probes in order to eliminate cross-talk between the probes so that simultaneous detection of different types of flaws at different depths can be achieved. In contrast to the previous uses of ferromagnetic materials purely as magnetic shields, an electromagnetic flaw detector recently developed at NASA Langley Research Center takes advantage of the flux focusing properties of a ferromagnetic mild steel in order to produce a simple, effective device for the non-destructive evaluation of conducting materials. The Flux Focusing Eddy Current Probe has been shown to accurately measure material thickness and fatigue damage. The straight forward flaw response of the probe makes the device ideal for rapid inspection of large structures, and has lead to its incorporation in a computer controlled search routine to locate fatigue crack tips and monitor experimental fatigue crack growth experiments.

Challenges in converting an interviewer-administered food probe database to self-administration in the National Cancer Institute Automated Self-administered 24-Hour Recall (ASA24).

PubMed

Zimmerman, Thea Palmer; Hull, Stephen G; McNutt, Suzanne; Mittl, Beth; Islam, Noemi; Guenther, Patricia M; Thompson, Frances E; Potischman, Nancy A; Subar, Amy F

2009-12-01

The National Cancer Institute (NCI) is developing an automated, self-administered 24-hour dietary recall (ASA24) application to collect and code dietary intake data. The goal of the ASA24 development is to create a web-based dietary interview based on the US Department of Agriculture (USDA) Automated Multiple Pass Method (AMPM) instrument currently used in the National Health and Nutrition Examination Survey (NHANES). The ASA24 food list, detail probes, and portion probes were drawn from the AMPM instrument; portion-size pictures from Baylor College of Medicine's Food Intake Recording Software System (FIRSSt) were added; and the food code/portion code assignments were linked to the USDA Food and Nutrient Database for Dietary Studies (FNDDS). The requirements that the interview be self-administered and fully auto-coded presented several challenges as the AMPM probes and responses were linked with the FNDDS food codes and portion pictures. This linking was accomplished through a "food pathway," or the sequence of steps that leads from a respondent's initial food selection, through the AMPM probes and portion pictures, to the point at which a food code and gram weight portion size are assigned. The ASA24 interview database that accomplishes this contains more than 1,100 food probes and more than 2 million food pathways and will include about 10,000 pictures of individual foods depicting up to 8 portion sizes per food. The ASA24 will make the administration of multiple days of recalls in large-scale studies economical and feasible.

NONDESTRUCTIVE EDDY CURRENT TESTING

DOEpatents

Renken, C.J. Jr.

1961-05-23

An eddy current testing device is described for measuring metal continuity independent of probe-to-sample spacing. An inductance would test probe is made a leg of a variable impedance bridge and the bridge is balanced with the probe away from the sample. An a-c signal is applied across the input terminals of the bridge circuit. As the probe is brought into proximity with the metal sample, the resulting impedance change in the probe gives an output signal from the bridge whose phase angle is proportional to the sample continuity and amplitude is proportional to the probe-tosample spacing. The output signal from the bridge is applied to a compensating network where, responsive to amplitude changes from the bridge output signal, a constant phased voltage output is maintained when the sample is continuous regardless of probe-to-sample spacing. A phase meter calibrated to read changes in resistivity of the metal sample measures the phase shift between the output of the compensating network and the original a-c signal applied to the bridge.

Micro- and nanodevices integrated with biomolecular probes.

PubMed

Alapan, Yunus; Icoz, Kutay; Gurkan, Umut A

2015-12-01

Understanding how biomolecules, proteins and cells interact with their surroundings and other biological entities has become the fundamental design criterion for most biomedical micro- and nanodevices. Advances in biology, medicine, and nanofabrication technologies complement each other and allow us to engineer new tools based on biomolecules utilized as probes. Engineered micro/nanosystems and biomolecules in nature have remarkably robust compatibility in terms of function, size, and physical properties. This article presents the state of the art in micro- and nanoscale devices designed and fabricated with biomolecular probes as their vital constituents. General design and fabrication concepts are presented and three major platform technologies are highlighted: microcantilevers, micro/nanopillars, and microfluidics. Overview of each technology, typical fabrication details, and application areas are presented by emphasizing significant achievements, current challenges, and future opportunities. Copyright © 2015 Elsevier Inc. All rights reserved.

Gamma Ray Bursts as Cosmological Probes with EXIST

NASA Astrophysics Data System (ADS)

Hartmann, Dieter; EXIST Team

2006-12-01

The EXIST mission, studied as a Black Hole Finder Probe within NASA's Beyond Einstein Program, would, in its current design, trigger on 1000 Gamma Ray Bursts (GRBs) per year (Grindlay et al, this meeting). The redshift distribution of these GRBs, using results from Swift as a guide, would probe the z > 7 epoch at an event rate of > 50 per year. These bursts trace early cosmic star formation history, point to a first generation of stellar objects that reionize the universe, and provide bright beacons for absorption line studies with groundand space-based observatories. We discuss how EXIST, in conjunction with other space missions and future large survey programs such as LSST, can be utilized to advance our understanding of cosmic chemical evolution, the structure and evolution of the baryonic cosmic web, and the formation of stars in low metallicity environments.

HIDRA-MAT: A Material Analysis Tool for Fusion Devices

NASA Astrophysics Data System (ADS)

Andruczyk, Daniel; Rizkallah, Rabel; Bedoya, Felipe; Kapat, Aveek; Schamis, Hanna; Allain, Jean Paul

2017-10-01

The former WEGA stellarator which is now operating as HIDRA at the University of Illinois will be almost exclusively used to study the intimate relationship between the plasma interacting with surfaces of different materials. A Material Analysis Tool (HIDRA-MAT) is being designed and will be built based on the successful Material Analysis and Particle Probe (MAPP) which is currently used on NSTX-U at PPPL. This will be an in-situ material diagnostic probe, meaning that all analysis can be done without breaking vacuum. This allows surface changes to be studied in real-time. HIDRA-MAT will consist of several in-situ diagnostics including Langmuir probes (LP), Thermal Desorption Spectroscopy (TDS), X-ray Photo Spectroscopy (XPS) and Ion Scattering Spectroscopy (ISS). This presentation will outline the HIDRA-MAT diagnostic and initial design, as well as its integration into the HIDRA system.

Double-labeled donor probe can enhance the signal of fluorescence resonance energy transfer (FRET) in detection of nucleic acid hybridization

PubMed Central

Okamura, Yukio; Kondo, Satoshi; Sase, Ichiro; Suga, Takayuki; Mise, Kazuyuki; Furusawa, Iwao; Kawakami, Shigeki; Watanabe, Yuichiro

2000-01-01

A set of fluorescently-labeled DNA probes that hybridize with the target RNA and produce fluorescence resonance energy transfer (FRET) signals can be utilized for the detection of specific RNA. We have developed probe sets to detect and discriminate single-strand RNA molecules of plant viral genome, and sought a method to improve the FRET signals to handle in vivo applications. Consequently, we found that a double-labeled donor probe labeled with Bodipy dye yielded a remarkable increase in fluorescence intensity compared to a single-labeled donor probe used in an ordinary FRET. This double-labeled donor system can be easily applied to improve various FRET probes since the dependence upon sequence and label position in enhancement is not as strict. Furthermore this method could be applied to other nucleic acid substances, such as oligo RNA and phosphorothioate oligonucleotides (S-oligos) to enhance FRET signal. Although the double-labeled donor probes labeled with a variety of fluorophores had unexpected properties (strange UV-visible absorption spectra, decrease of intensity and decay of donor fluorescence) compared with single-labeled ones, they had no relation to FRET enhancement. This signal amplification mechanism cannot be explained simply based on our current results and knowledge of FRET. Yet it is possible to utilize this double-labeled donor system in various applications of FRET as a simple signal-enhancement method. PMID:11121494

Subminiature eddy current transducers for studying metal- dielectric junctions

NASA Astrophysics Data System (ADS)

Dmitriev, S.; Katasonov, A.; Malikov, V.; Sagalakov, A.; Davydchenko, M.; Shevtsova, L.; Ishkov, A.

2016-11-01

Based on an eddy current transducer (ECT), a probe has been designed to research metal-dielectric structures. The measurement procedure allowing one to detect defects in laminate composites with a high accuracy is described. The transducer was tested on the layered structure consisting of paper and aluminum layers with a thickness of 100 μm each in which the model defect was placed. The dependences of the ECT signal on the defect in this structure are given.

Measurements of monopolar and bipolar current spreads using forward-masking with a fixed probe.

PubMed

Bingabr, Mohamed G; Espinoza-Varas, Blas; Sigdel, Saroj

2014-05-01

This research employed a forward-masking paradigm to estimate the current spread of monopolar (MP) and bipolar (BP) maskers, with current amplitudes adjusted to elicit the same loudness. Since the spatial separation between active and return electrodes is smaller in BP than in MP configurations, the BP current spread is more localized and presumably superior in terms of speech intelligibility. Because matching the loudness requires higher current in BP than in MP stimulation, previous forward-masking studies show that BP current spread is not consistently narrower across subjects or electrodes within a subject. The present forward-masking measures of current spread differ from those of previous studies by using the same BP probe electrode configuration for both MP and BP masker configurations, and adjusting the current levels of the MP and BP maskers so as to match them in loudness. With this method, the estimate of masker current spread would not be contaminated by differences in probe current spread. Forward masking was studied in four cochlear implant patients, two females and two males, with speech recognition scores higher than 50%; that is, their auditory-nerve survival status was more than adequate to carry out the experiments. The data showed that MP and BP masker configurations produce equivalent masking patterns (and current spreads) in three participants. A fourth participant displayed asymmetrical patterns with enhancement rather than masking in some cases, especially when the probe and masker were at the same location. This study showed equivalent masking patterns for MP and BP maskers when the BP masker current amplitude was increased to match the loudness of the MP masker, and the same BP probe configuration is used with both maskers. This finding could help to explain why cochlear implant users often fail to accrue higher speech intelligibility benefit from BP stimulation.

Precise and selective sensing of DNA-DNA hybridization by graphene/Si-nanowires diode-type biosensors.

PubMed

Kim, Jungkil; Park, Shin-Young; Kim, Sung; Lee, Dae Hun; Kim, Ju Hwan; Kim, Jong Min; Kang, Hee; Han, Joong-Soo; Park, Jun Woo; Lee, Hosun; Choi, Suk-Ho

2016-08-18

Single-Si-nanowire (NW)-based DNA sensors have been recently developed, but their sensitivity is very limited because of high noise signals, originating from small source-drain current of the single Si NW. Here, we demonstrate that chemical-vapor-deposition-grown large-scale graphene/surface-modified vertical-Si-NW-arrays junctions can be utilized as diode-type biosensors for highly-sensitive and -selective detection of specific oligonucleotides. For this, a twenty-seven-base-long synthetic oligonucleotide, which is a fragment of human DENND2D promoter sequence, is first decorated as a probe on the surface of vertical Si-NW arrays, and then the complementary oligonucleotide is hybridized to the probe. This hybridization gives rise to a doping effect on the surface of Si NWs, resulting in the increase of the current in the biosensor. The current of the biosensor increases from 19 to 120% as the concentration of the target DNA varies from 0.1 to 500 nM. In contrast, such biosensing does not come into play by the use of the oligonucleotide with incompatible or mismatched sequences. Similar results are observed from photoluminescence microscopic images and spectra. The biosensors show very-uniform current changes with standard deviations ranging ~1 to ~10% by ten-times endurance tests. These results are very promising for their applications in accurate, selective, and stable biosensing.

Probes for measuring noise current in an electronic cable

NASA Technical Reports Server (NTRS)

Lundy, C. C.

1974-01-01

Electromagnetic interference in deep-space network receiver is often caused by stray coupling from power lines. These stray signals create potential differences between ground terminals, which leads to excessive noise in receiver circuits. Pair of probes detect and measure noise currents in conductors.

Rosetta Langmuir Probe Photoelectron Emission and Solar Ultraviolet Flux at Comet 67P

NASA Astrophysics Data System (ADS)

Johansson, F. L.; Odelstad, E.; Paulsson, J. J.; Harang, S. S.; Eriksson, A. I.; Mannel, T.; Vigren, E.; Edberg, N. J. T.; Miloch, W. J.; Simon Wedlund, C.; Thiemann, E.; Epavier, F.; Andersson, L.

2017-12-01

The Langmuir Probe instrument on Rosetta monitored the photoelectron emission current of the probes during the Rosetta mission at comet 67P/Churyumov-Gerasimenko, in essence acting as a photodiode monitoring the solar ultraviolet radiation at wavelengths below 250 nm. We have used three methods of extracting the photoelectron saturation current from the Langmuir probe measurements. The resulting dataset can be used as an index of the solar far and extreme ultraviolet at the Rosetta spacecraft position, including flares, in wavelengths that are important for photoionisation of the cometary neutral gas. Comparing the photoemission current to data measurements by MAVEN/EUVM and TIMED/SEE, we find good correlation when 67P was at large heliocentric distances early and late in the mission, but up to 50 percent decrease of the expected photoelectron current at perihelion. We discuss possible reasons for the photoemission decrease, including scattering and absorption by nanograins created by disintegration of cometary dust far away from the nucleus.

Transmit-receive eddy current probes for defect detection and sizing in steam generator tubes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Obrutsky, L.S.; Cecco, V.S.; Sullivan, S.P.

1997-02-01

Inspection of steam generator tubes in aging Nuclear Generating Stations is increasingly important. Defect detection and sizing, especially in defect prone areas such as the tubesheet, support plates and U-bend regions, are required to assess the fitness-for-service of the steam generators. Information about defect morphology is required to address operational integrity issues, i.e., risk of tube rupture, number of tubes at risk, consequential leakage. A major challenge continues to be the detection and sizing of circumferential cracks. Utilities around the world have experienced this type of tube failure. Conventional in-service inspection, performed with eddy current bobbin probes, is ineffectual inmore » detecting circumferential cracks in tubing. It has been demonstrated in CANDU steam generators, with deformation, magnetite and copper deposits that multi-channel probes with transmit-receive eddy current coils are superior to those using surface impedance coils. Transmit-receive probes have strong directional properties, permitting probe optimization according to crack orientation. They are less sensitive to lift-off noise and magnetite deposits and possess good discrimination to internal defects. A single pass C3 array transmit-receive probe developed by AECL can detect and size circumferential stress corrosion cracks as shallow as 40% through-wall. Since its first trial in 1992, it has been used routinely for steam generator in-service inspection of four CANDU plants, preventing unscheduled shutdowns due to leaking steam generator tubes. More recently, a need has surfaced for simultaneous detection of both circumferential and axial cracks. The C5 probe was designed to address this concern. It combines transmit-receive array probe technology for equal sensitivity to axial and circumferential cracks with a bobbin probe for historical reference. This paper will discuss the operating principles of transmit-receive probes, along with inspection results.« less

Design and modeling of a planar probe for power measurements in a capacitive plasma sheath

NASA Astrophysics Data System (ADS)

Gahan, D.; Hopkins, M. B.; Ellingboe, A. R.

2004-09-01

The design and modeling of a planar probe for power measurement in a capacitive RF sheath is described. The probe is to be biased negatively, using a DC power supply, while simultaneously being driven with an RF voltage. A simple model has been developed which describes the voltage, current and impedance from the generator to the probe surface incorporating the transmission line. A conventional method to determine the power through such a probe would be to measure the voltage, current and their phase relationship very close to the probe surface. This can be very difficult to do with much accuracy since the load is almost purely reactive. An alternative method is discussed. The model shows that for certain lengths of transmission line there exists a point on that transmission line where the imaginary impedance goes to zero. If the power is measured at this point where the current and voltage are almost in phase the result should be more accurate. A brief description of the model is given along with some results for its validation. The operation of the power sensor used is also explained.

Conflict Probe Concepts Analysis in Support of Free Flight

NASA Technical Reports Server (NTRS)

Warren, Anthony W.; Schwab, Robert W.; Geels, Timothy J.; Shakarian, Arek

1997-01-01

This study develops an operational concept and requirements for en route Free Flight using a simulation of the Cleveland Air Route Traffic Control Center, and develops requirements for an automated conflict probe for use in the Air Traffic Control (ATC) Centers. In this paper, we present the results of simulation studies and summarize implementation concepts and infrastructure requirements to transition from the current air traffic control system to mature Free Right. The transition path to Free Flight envisioned in this paper assumes an orderly development of communications, navigation, and surveillance (CNS) technologies based on results from our simulation studies. The main purpose of this study is to provide an overall context and methodology for evaluating airborne and ground-based requirements for cooperative development of the future ATC system.

Filling America's fiber intake gap: Summary of a roundtable to probe realistic solutions with a focus on grain-based foods

USDA-ARS?s Scientific Manuscript database

Current fiber intakes are alarmingly low, with long-term implications for public health related to risk of coronary heart disease, stroke, hypertension, certain gastrointestinal disorders, obesity, and the continuum of metabolic dysfunctions including prediabetes and type 2 diabetes. With more than ...

Using oligonucleotide aptamer probes for immunostaining of formalin-fixed and paraffin-embedded tissues

PubMed Central

Zeng, Zihua; Zhang, Peng; Zhao, Nianxi; Sheehan, Andrea M; Tung, Ching-Hsuan; Chang, Chung-Che; Zu, Youli

2011-01-01

For tissue immunostaining, antibodies are currently the only clinically validated and commercially available probes. Aptamers, which belong to a class of small molecule ligands composed of short single-stranded oligonucleotides, have emerged as probes over the last several decades; however, their potential clinical value has not yet been fully explored. Using cultured cells and an RNA-based CD30 aptamer, we recently demonstrated that the synthetic aptamer is useful as a specific probe for flow cytometric detection of CD30-expressing lymphoma cells. In this study, we further validated the use of this aptamer probe for immunostaining of formalin-fixed and paraffin-embedded lymphoma tissues. Using CD30 antibody as a standard control, we demonstrated that the synthetic CD30 aptamer specifically recognized and immunostained tumor cells of classical Hodgkin lymphoma and anaplastic large cell lymphoma, but did not react with background cells within tumor sites. Notably, the CD30 aptamer probe optimally immunostained lymphoma cells with lower temperature antigen retrieval (37 vs 96°C for antibody) and shorter probing reaction times (20 vs 90 min for antibody) than typical antibody immunostaining protocols. In addition, the CD30 aptamer probe showed no nonspecific background staining of cell debris in necrotic tissue and exhibited no cross-reaction to tissues that do not express CD30, as confirmed by a standard CD30 antibody staining. Therefore, our findings indicate that the synthetic oligonucleotide CD30 aptamer can be used as a probe for immunostaining of fixed tissue sections for disease diagnosis. PMID:20693984

Electroanalytical study of proflavine intercalation in 5-methyl or inosine-containing amplicons.

PubMed

Alexiadou, Despina K; Ioannou, Andrea K; Kouidou-Andreou, Sofia A; Voulgaropoulos, Anastasios N; Girousi, Stella Th

2008-10-01

Amplicons corresponding to the GC-rich p53 exon 5 and its analogues, synthesized by substituting 60% of cytosine by 5-methyl-cytosine, or 60% of guanosine by inosine and GC-poor p53 exon 6 were synthesized and investigated electrochemically, in the presence and absence of proflavine, by differential pulse voltammetry (DPV). Incorporation of base analogues and the thermal stability of the resulting amplicons were tested in the presence of a fluorescent probe (Sybr-Green). Peak current at 1.0 V was lower for methylated than for unmethylated PCR amplicons and was similarly affected by proflavine intercalation. In contrast, considerable peak current differences were observed in the presence of proflavine for unmodified exon 5 v.s. exon 6 or inosine-containing amplicons. Thermal analysis verified the expected shifts in melting temperature (T (m)) due to the base analogue incorporation and GC-content variations. In conclusion, methylated and unmethylated PCR amplicons could be distinguished in model DNA systems using differential pulse voltammetry (DPV) and use of proflavine could serve as an electrochemical probe for identifying different DNA conformations.

A sandwich-like differential B-dot based on EACVD polycrystalline diamond slice

NASA Astrophysics Data System (ADS)

Xu, P.; Yu, Y.; Xu, L.; Zhou, H. Y.; Qiu, C. J.

2018-06-01

In this article, we present a method of mass production of a standardized high-performance differential B-dot magnetic probe together with the magnetic field measurement in a pulsed current device with the current up to hundreds of kilo-Amperes. A polycrystalline diamond slice produced in an Electron Assisted Chemical Vapor Deposition device is used as the base and insulating material to imprint two symmetric differential loops for the magnetic field measurement. The SP3 carbon bond in the cubic lattice structure of diamond is confirmed by Raman spectra. The thickness of this slice is 20 μm. A gold loop is imprinted onto each surface of the slice by using the photolithography technique. The inner diameter, width, and thickness of each loop are 0.8 mm, 50 μm, and 1 μm, respectively. It provides a way of measuring the pulsed magnetic field with a high spatial and temporal resolution, especially in limited space. This differential magnetic probe has demonstrated a very good common-mode rejection rate through the pulsed magnetic field measurement.

Biophysical characterization of the fluorescent protein voltage probe VSFP2.3 based on the voltage-sensing domain of Ci-VSP.

PubMed

Lundby, Alicia; Akemann, Walther; Knöpfel, Thomas

2010-11-01

A voltage sensitive phosphatase was discovered in the ascidian Ciona intestinalis. The phosphatase, Ci-VSP, contains a voltage-sensing domain homologous to those known from voltage-gated ion channels, but unlike ion channels, the voltage-sensing domain of Ci-VSP can reside in the cell membrane as a monomer. We fused the voltage-sensing domain of Ci-VSP to a pair of fluorescent reporter proteins to generate a genetically encodable voltage-sensing fluorescent probe, VSFP2.3. VSFP2.3 is a fluorescent voltage probe that reports changes in membrane potential as a FRET (fluorescence resonance energy transfer) signal. Here we report sensing current measurements from VSFP2.3, and show that VSFP2.3 carries 1.2 e sensing charges, which are displaced within 1.5 ms. The sensing currents become faster at higher temperatures, and the voltage dependence of the decay time constants is temperature dependent. Neutralization of an arginine in S4, previously suggested to be a sensing charge, and measuring associated sensing currents indicate that this charge is likely to reside at the membrane-aqueous interface rather than within the membrane electric field. The data presented give us insights into the voltage-sensing mechanism of Ci-VSP, which will allow us to further improve the sensitivity and kinetics of the family of VSFP proteins.

Probing ultra-fast processes with high dynamic range at 4th-generation light sources: Arrival time and intensity binning at unprecedented repetition rates

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kovalev, S.; Green, B.; Golz, T.

Here, understanding dynamics on ultrafast timescales enables unique and new insights into important processes in the materials and life sciences. In this respect, the fundamental pump-probe approach based on ultra-short photon pulses aims at the creation of stroboscopic movies. Performing such experiments at one of the many recently established accelerator-based 4th-generation light sources such as free-electron lasers or superradiant THz sources allows an enormous widening of the accessible parameter space for the excitation and/or probing light pulses. Compared to table-top devices, critical issues of this type of experiment are fluctuations of the timing between the accelerator and external laser systemsmore » and intensity instabilities of the accelerator-based photon sources. Existing solutions have so far been only demonstrated at low repetition rates and/or achieved a limited dynamic range in comparison to table-top experiments, while the 4th generation of accelerator-based light sources is based on superconducting radio-frequency technology, which enables operation at MHz or even GHz repetition rates. In this article, we present the successful demonstration of ultra-fast accelerator-laser pump-probe experiments performed at an unprecedentedly high repetition rate in the few-hundred-kHz regime and with a currently achievable optimal time resolution of 13 fs (rms). Our scheme, based on the pulse-resolved detection of multiple beam parameters relevant for the experiment, allows us to achieve an excellent sensitivity in real-world ultra-fast experiments, as demonstrated for the example of THz-field-driven coherent spin precession.« less

Probing ultra-fast processes with high dynamic range at 4th-generation light sources: Arrival time and intensity binning at unprecedented repetition rates

DOE PAGES

Kovalev, S.; Green, B.; Golz, T.; ...

2017-03-06

Here, understanding dynamics on ultrafast timescales enables unique and new insights into important processes in the materials and life sciences. In this respect, the fundamental pump-probe approach based on ultra-short photon pulses aims at the creation of stroboscopic movies. Performing such experiments at one of the many recently established accelerator-based 4th-generation light sources such as free-electron lasers or superradiant THz sources allows an enormous widening of the accessible parameter space for the excitation and/or probing light pulses. Compared to table-top devices, critical issues of this type of experiment are fluctuations of the timing between the accelerator and external laser systemsmore » and intensity instabilities of the accelerator-based photon sources. Existing solutions have so far been only demonstrated at low repetition rates and/or achieved a limited dynamic range in comparison to table-top experiments, while the 4th generation of accelerator-based light sources is based on superconducting radio-frequency technology, which enables operation at MHz or even GHz repetition rates. In this article, we present the successful demonstration of ultra-fast accelerator-laser pump-probe experiments performed at an unprecedentedly high repetition rate in the few-hundred-kHz regime and with a currently achievable optimal time resolution of 13 fs (rms). Our scheme, based on the pulse-resolved detection of multiple beam parameters relevant for the experiment, allows us to achieve an excellent sensitivity in real-world ultra-fast experiments, as demonstrated for the example of THz-field-driven coherent spin precession.« less

Eddy current testing probe with dual half-cylindrical coils

NASA Astrophysics Data System (ADS)

Bae, Byung-Hoon; Choi, Jung-Mi; Kim, Soo-Yong

2000-02-01

We have developed a new eddy current probe composed of a dual half-cylindrical (2HC) coil as an exciting coil and a sensing coil that is placed in the small gap of the 2HC coil. The 2HC coil induces a linear eddy current on the narrow region within the target medium. The magnitude of eddy current has a maximum peak with the narrow width, underneath the center of the exciting 2HC coil. Because of the linear eddy current, the probe can be used to detect not only the existence of a crack but also its direction in conducting materials. Using specimen with a machined crack, and varying the exciting frequency from 0.5 to 100 kHz, we investigated the relationships between the direction of crack and the output voltage of the sensing coil.

DNA and RNA sequencing by nanoscale reading through programmable electrophoresis and nanoelectrode-gated tunneling and dielectric detection

DOEpatents

Lee, James W.; Thundat, Thomas G.

2005-06-14

An apparatus and method for performing nucleic acid (DNA and/or RNA) sequencing on a single molecule. The genetic sequence information is obtained by probing through a DNA or RNA molecule base by base at nanometer scale as though looking through a strip of movie film. This DNA sequencing nanotechnology has the theoretical capability of performing DNA sequencing at a maximal rate of about 1,000,000 bases per second. This enhanced performance is made possible by a series of innovations including: novel applications of a fine-tuned nanometer gap for passage of a single DNA or RNA molecule; thin layer microfluidics for sample loading and delivery; and programmable electric fields for precise control of DNA or RNA movement. Detection methods include nanoelectrode-gated tunneling current measurements, dielectric molecular characterization, and atomic force microscopy/electrostatic force microscopy (AFM/EFM) probing for nanoscale reading of the nucleic acid sequences.

Triazole-based Zn²⁺-specific molecular marker for fluorescence bioimaging.

PubMed

Sinha, Sougata; Mukherjee, Trinetra; Mathew, Jomon; Mukhopadhyay, Subhra K; Ghosh, Subrata

2014-04-25

Fluorescence bioimaging potential, both in vitro and in vivo, of a yellow emissive triazole-based molecular marker has been investigated and demonstrated. Three different kinds of cells, viz Bacillus thuringiensis, Candida albicans, and Techoma stans pollen grains were used to investigate the intracellular zinc imaging potential of 1 (in vitro studies). Fluorescence imaging of translocation of zinc through the stem of small herb, Peperomia pellucida, having transparent stem proved in vivo bioimaging capability of 1. This approach will enable in screening cell permeability and biostability of a newly developed probe. Similarly, the current method for detection and localization of zinc in Gram seed sprouts could be an easy and potential alternative of the existing analytical methods to investigate the efficiency of various strategies applied for increasing zinc-content in cereal crops. The probe-zinc ensemble has efficiently been applied for detecting phosphate-based biomolecules. Copyright © 2014 Elsevier B.V. All rights reserved.

Design and validation of the ball-pen probe for measurements in a low-temperature magnetized plasma

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bousselin, G.; Cavalier, J.; Pautex, J. F.

Ball-pen probes have been used in fusion devices for direct measurements of the plasma potential. Their application in low-temperature magnetized plasma devices is still subject to studies. In this context, a ball-pen probe has been recently implemented on the linear plasma device Mirabelle. Produced by a thermionic discharge, the plasma is characterized by a low electron temperature and a low density. Plasma confinement is provided by an axial magnetic field that goes up to 100 mT. The principle of the ball-pen probe is to adjust the saturation current ratio to 1 by reducing the electron current contribution. In that case,more » the floating potential of the probe is close to the plasma potential. A thorough study of the ball-pen probe operation is performed for different designs of the probe over a large set of plasma conditions. Comparisons between ball-pen, Langmuir, and emissive probes are conducted in the same plasma conditions. The ball-pen probe is successfully measuring the plasma potential in these specific plasma conditions only if an adapted electronics and an adapted probe size to the plasma characteristic lengths ({lambda}{sub D}, {rho}{sub ce}) are used.« less

Smart skin technology development for measuring ice accretion, stall, and high AOA aircraft performance. Part 1: Capacitive ice detector development

NASA Technical Reports Server (NTRS)

Pruzan, Daniel A.; Khatkhate, Ateen A.; Gerardi, Joseph J.; Hickman, Gail A.

1993-01-01

A reliable way to detect and measure ice accretion during flight is required to reduce the hazards of icing currently threatening present day aircraft. Many of the sensors used for this purpose are invasive (probe) sensors which must be placed in areas of the airframe where ice does not naturally form. Due to the difference in capture efficiency of the exposed surface, difficulties result in correlating the ice accretion on the probe to what is happening on a number of vastly different airfoil sections. Most flush mounted sensors in use must be integrated into the aircraft surface by cutting or drilling the aircraft surface. An alternate type of ice detector which is based on a NASA patent is currently being investigated at Innovative Dynamics, Inc. (IDI). Results of the investigation into the performance of different capacitive type sensor designs, both rigid as well as elastic, are presented.

Analyte-Triggered DNA-Probe Release from a Triplex Molecular Beacon for Nanopore Sensing.

PubMed

Guo, Bingyuan; Sheng, Yingying; Zhou, Ke; Liu, Quansheng; Liu, Lei; Wu, Hai-Chen

2018-03-26

A new nanopore sensing strategy based on triplex molecular beacon was developed for the detection of specific DNA or multivalent proteins. The sensor is composed of a triplex-forming molecular beacon and a stem-forming DNA component that is modified with a host-guest complex. Upon target DNA hybridizing with the molecular beacon loop or multivalent proteins binding to the recognition elements on the stem, the DNA probe is released and produces highly characteristic current signals when translocated through α-hemolysin. The frequency of current signatures can be used to quantify the concentrations of the target molecules. This sensing approach provides a simple, quick, and modular tool for the detection of specific macromolecules with high sensitivity and excellent selectivity. It may find useful applications in point-of-care diagnostics with a portable nanopore kit in the future. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Conductive-probe atomic force microscopy characterization of silicon nanowire

PubMed Central

2011-01-01

The electrical conduction properties of lateral and vertical silicon nanowires (SiNWs) were investigated using a conductive-probe atomic force microscopy (AFM). Horizontal SiNWs, which were synthesized by the in-plane solid-liquid-solid technique, are randomly deployed into an undoped hydrogenated amorphous silicon layer. Local current mapping shows that the wires have internal microstructures. The local current-voltage measurements on these horizontal wires reveal a power law behavior indicating several transport regimes based on space-charge limited conduction which can be assisted by traps in the high-bias regime (> 1 V). Vertical phosphorus-doped SiNWs were grown by chemical vapor deposition using a gold catalyst-driving vapor-liquid-solid process on higly n-type silicon substrates. The effect of phosphorus doping on the local contact resistance between the AFM tip and the SiNW was put in evidence, and the SiNWs resistivity was estimated. PMID:21711623

Probing local work function of electron emitting Si-nanofacets

NASA Astrophysics Data System (ADS)

Basu, Tanmoy; Som, Tapobrata

2017-10-01

Large area, Si-nanofacets are synthesized by obliquely incident low energy Ar+-ion-beam bombardment at room temperature (RT). The field emission properties of such nanofacets are studied based on current-voltage measurements and the Fowler-Nordheim equation. Low turn-on field with relatively high current density is obtained due to the shape and an overall rough morphology. We demonstrate a tunable field emission property from the silicon nanofacets by varying the ion exposure time. Atomic force microscopy (AFM) in conjunction with Kelvin probe force microscopy (KPFM) measurements provide the information on the aspect ratio and confirms the presence of native oxide layer near the apexes of the facets, respectively. The inhomogeneous oxidation leads to an increase in the local work function at the apexes of the facets, restricting the electron emission from the same. Due to its room temperature fabrication, the present method is of great significance to the low-cost vacuum field emission devices fabrication.

Tidally-driven effluent detected by long-term temperature monitoring at the TAG hydrothermal mound, Mid-Atlantic Ridge

NASA Astrophysics Data System (ADS)

Kinoshita, M.; Von Herzen, R. P.; Matsubayashi, O.; Fujioka, K.

1998-06-01

During Aug. 13-21, 1994, temperatures and current velocity were simultaneously monitored on the TAG hydrothermal mound. Three `Giant Kelps (GKs)', vertical thermistor arrays of 50 m height, were moored on the periphery of the central black smoker complex (CBC). A `Manatee', multi-monitoring system including current velocity, was deployed 50 m east of CBC. Four `Daibutsu' geothermal probes penetrated the sediment south to west of CBC. Compilation of all data revealed semi-diurnal variations in water temperatures and current velocity, and allowed us to discuss the source of these anomalies. Temperature anomalies of GKs correlate well with current velocity, and are interpreted to be caused by the main plume from CBC that was bent over by the tidal current. We identified two types of asymmetric, periodic temperature variations at Daibutsu Probes 2 and 8, located 20 m to the south of CBC. By comparing temperatures and current velocity, they are attributed to non-buoyant effluents laterally advected by the tidal current. The source of one variation is located east to ESE of the probes, and the source of the other is located to the north. On Aug. 31, a new periodic anomaly emerged on Probe 2 with its amplitude up to 0.8°C. The 6-h offset between the new anomaly and the previous one suggests that the source of the new anomaly lies to the west of Probe 2. The heat flux of these non-buoyant effluents is estimated to range from 30 to 100 kW/m 2, which is of the same order as direct estimates of diffuse flow at the TAG mound. It suggests that a significant amount of diffuse effluent is laterally advected by the prevailing current near the seafloor.

X-Ray Probes of Cosmic Star Formation History

NASA Technical Reports Server (NTRS)

Ghosh, Pranab; White, Nicholas E.

2001-01-01

We discuss the imprints left by a cosmological evolution of the star formation rate (SFR) on the evolution of X-ray luminosities Lx of normal galaxies, using the scheme earlier proposed by us, wherein the evolution of LX of a galaxy is driven by the evolution of its X-ray binary population. As indicated in our earlier work, the profile of Lx with redshift can both serve as a diagnostic probe of the SFR profile and constrain evolutionary models for X-ray binaries. We report here the first calculation of the expected evolution of X-ray luminosities of galaxies, updating our work by using a suite of more recently developed SFR profiles that span the currently plausible range. The first Chandra deep imaging results on Lx evolution are beginning to probe the SFR profile of bright spiral galaxies; the early results are consistent with predictions based on current SFR models. Using these new SFR profiles, the resolution of the "birthrate problem" of low-mass X-ray binaries and recycled, millisecond pulsars in terms of an evolving global SFR is more complete. We discuss the possible impact of the variations in the SFR profile of individual galaxies and galaxy types.

Detection of single-nucleotide polymorphisms using gold nanoparticles and single-strand-specific nucleases.

PubMed

Chen, Yen-Ting; Hsu, Chiao-Ling; Hou, Shao-Yi

2008-04-15

The current study reports an assay approach that can detect single-nucleotide polymorphisms (SNPs) and identify the position of the point mutation through a single-strand-specific nuclease reaction and a gold nanoparticle assembly. The assay can be implemented via three steps: a single-strand-specific nuclease reaction that allows the enzyme to truncate the mutant DNA; a purification step that uses capture probe-gold nanoparticles and centrifugation; and a hybridization reaction that induces detector probe-gold nanoparticles, capture probe-gold nanoparticles, and the target DNA to form large DNA-linked three-dimensional aggregates of gold nanoparticles. At high temperature (63 degrees C in the current case), the purple color of the perfect match solution would not change to red, whereas a mismatched solution becomes red as the assembled gold nanoparticles separate. Using melting analysis, the position of the point mutation could be identified. This assay provides a convenient colorimetric detection that enables point mutation identification without the need for expensive mass spectrometry. To our knowledge, this is the first report concerning SNP detection based on a single-strand-specific nuclease reaction and a gold nanoparticle assembly.

Design of a Miniaturized Langmuir Plasma Probe for the QuadSat/PnP

NASA Astrophysics Data System (ADS)

Landavazo, M.; Jorgensen, A. M.; Del Barga, C.; Ferguson, D.; Guillette, D.; Huynh, A.; Klepper, J.; Kuker, J.; Lyke, J. C.; Marohn, B.; Mason, J.; Quiroga, J.; Ravindran, V.; Yelton, C.; Zagrai, A. N.; Zufelt, B.

2011-12-01

We have developed a miniaturized Langmuir plasma probe for measuring plasma density in low-earth orbit. Measuring plasma density in the upper ionosphere is important as a diagnostic for the rest of the ionosphere and as an input to space weather forecasting models. Developing miniaturized instrumentation allows easier deployment of a large number of small satellites for monitoring space weather. Our instrument was designed for the Swedish QuadSat/PnP, with the following constraints: A volume constraint of 5x5x1.25cm for the electronics enclosure, a mass budget 100 g, and a power budget of 0.5 W. We met the volume and mass constraints and where able to use less power than budgeted, only 0.25 W. We designed the probe for a bias range of +/-15V and current measurements in the 1 nA to 1 mA range (6 orders of magnitude). Necessary voltage of +/- 15 V and 3.3 V were generated on-board from a single 5 V supply. The electronics suite is based off carefully selected yet affordable commercial components that exhibit low noise, low leakage currents and low power consumption. Size constraints, low noise and low leakage requirements called for a carefully designed four layer PCB with a properly guarded current path using surface mount components on both sides. An ultra-low power microcontroller handles instrument functionality and is fully controllable over i2c using SPA-1 space plug and play. We elected for a probe launched deployed, which required careful design to survive launch vibrations while staying within the mass budget. The QuadSat/PnP has not been launched at the time of writing. We will present details of the instrument design and initial calibration data.

Oligonucleotide probes functionalization of nanogap electrodes.

PubMed

Zaffino, Rosa Letizia; Mir, Mònica; Samitier, Josep

2017-11-01

Nanogap electrodes have attracted a lot of consideration as promising platform for molecular electronic and biomolecules detection. This is mainly for their higher aspect ratio, and because their electrical properties are easily accessed by current-voltage measurements. Nevertheless, application of standard current-voltages measurements used to characterize nanogap response, and/or to modify specific nanogap electrodes properties, represents an issue. Since the strength of electrical fields in nanoscaled devices can reach high values, even at low voltages. Here, we analyzed the effects induced by different methods of surface modification of nanogap electrodes, in test-voltage application, employed for the electrical detection of a desoxyribonucleic acid (DNA) target. Nanogap electrodes were functionalized with two antisymmetric oligo-probes designed to have 20 terminal bases complementary to the edges of the target, which after hybridization bridges the nanogap, closing the electrical circuit. Two methods of functionalization were studied for this purpose; a random self-assembling of a mixture of the two oligo-probes (OPs) used in the platform, and a selective method that controls the position of each OP at selected side of nanogap electrodes. We used for this aim, the electrophoretic effect induced on negatively charged probes by the application of an external direct current voltage. The results obtained with both functionalization methods where characterized and compared in terms of electrode surface covering, calculated by using voltammetry analysis. Moreover, we contrasted the electrical detection of a DNA target in the nanogap platform either in site-selective and in randomly assembled nanogap. According to our results, a denser, although not selective surface functionalization, is advantageous for such kind of applications. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Accounting for Debye sheath expansion for proud Langmuir probes in magnetic confinement fusion plasmas.

PubMed

Tsui, C K; Boedo, J A; Stangeby, P C

2018-01-01

A Child-Langmuir law-based method for accounting for Debye sheath expansion while fitting the current-voltage I-V characteristic of proud Langmuir probes (electrodes that extend into the volume of the plasma) is described. For Langmuir probes of a typical size used in tokamak plasmas, these new estimates of electron temperature and ion saturation current density values decreased by up to 60% compared to methods that did not account for sheath expansion. Changes to the collection area are modeled using the Child-Langmuir law and effective expansion perimeter l p , and the model is thus referred to as the "perimeter sheath expansion method." l p is determined solely from electrode geometry, so the method may be employed without prior measurement of the magnitude of the sheath expansion effects for a given Langmuir probe and can be used for electrodes of different geometries. This method correctly predicts the non-saturating ΔI/ΔV slope for cold, low-density plasmas where sheath-expansion effects are strong, as well as for hot plasmas where ΔI/ΔV ∼ 0, though it is shown that the sheath can still significantly affect the collection area in these hot conditions. The perimeter sheath expansion method has several advantages compared to methods where the non-saturating current is fitted: (1) It is more resilient to scatter in the I-V characteristics observed in turbulent plasmas. (2) It is able to separate the contributions to the ΔI/ΔV slope from sheath expansion to that of the high energy electron tail in high Te conditions. (3) It calculates the change in the collection area due to the Debye sheath for conditions where ΔI/ΔV ∼ 0 and for V = V f .

Accounting for Debye sheath expansion for proud Langmuir probes in magnetic confinement fusion plasmas

NASA Astrophysics Data System (ADS)

Tsui, C. K.; Boedo, J. A.; Stangeby, P. C.; TCV Team

2018-01-01

A Child-Langmuir law-based method for accounting for Debye sheath expansion while fitting the current-voltage I-V characteristic of proud Langmuir probes (electrodes that extend into the volume of the plasma) is described. For Langmuir probes of a typical size used in tokamak plasmas, these new estimates of electron temperature and ion saturation current density values decreased by up to 60% compared to methods that did not account for sheath expansion. Changes to the collection area are modeled using the Child-Langmuir law and effective expansion perimeter lp, and the model is thus referred to as the "perimeter sheath expansion method." lp is determined solely from electrode geometry, so the method may be employed without prior measurement of the magnitude of the sheath expansion effects for a given Langmuir probe and can be used for electrodes of different geometries. This method correctly predicts the non-saturating ΔI/ΔV slope for cold, low-density plasmas where sheath-expansion effects are strong, as well as for hot plasmas where ΔI/ΔV ˜ 0, though it is shown that the sheath can still significantly affect the collection area in these hot conditions. The perimeter sheath expansion method has several advantages compared to methods where the non-saturating current is fitted: (1) It is more resilient to scatter in the I-V characteristics observed in turbulent plasmas. (2) It is able to separate the contributions to the ΔI/ΔV slope from sheath expansion to that of the high energy electron tail in high Te conditions. (3) It calculates the change in the collection area due to the Debye sheath for conditions where ΔI/ΔV ˜ 0 and for V = Vf.

Estimating Highway Volumes Using Vehicle Probe Data - Proof of Concept: Preprint

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hou, Yi; Young, Stanley E; Sadabadi, Kaveh

This paper examines the feasibility of using sampled commercial probe data in combination with validated continuous counter data to accurately estimate vehicle volume across the entire roadway network, for any hour during the year. Currently either real time or archived volume data for roadways at specific times are extremely sparse. Most volume data are average annual daily traffic (AADT) measures derived from the Highway Performance Monitoring System (HPMS). Although methods to factor the AADT to hourly averages for typical day of week exist, actual volume data is limited to a sparse collection of locations in which volumes are continuously recorded.more » This paper explores the use of commercial probe data to generate accurate volume measures that span the highway network providing ubiquitous coverage in space, and specific point-in-time measures for a specific date and time. The paper examines the need for the data, fundamental accuracy limitations based on a basic statistical model that take into account the sampling nature of probe data, and early results from a proof of concept exercise revealing the potential of probe type data calibrated with public continuous count data to meet end user expectations in terms of accuracy of volume estimates.« less

Handheld probe for portable high frame photoacoustic/ultrasound imaging system

NASA Astrophysics Data System (ADS)

Daoudi, K.; van den Berg, P. J.; Rabot, O.; Kohl, A.; Tisserand, S.; Brands, P.; Steenbergen, W.

2013-03-01

Photoacoustics is a hybrid imaging modality that is based on the detection of acoustic waves generated by absorption of pulsed light by tissue chromophors. In current research, this technique uses large and costly photoacoustic systems with a low frame rate imaging. To open the door for widespread clinical use, a compact, cost effective and fast system is required. In this paper we report on the development of a small compact handset pulsed laser probe which will be connected to a portable ultrasound system for real-time photoacoustic imaging and ultrasound imaging. The probe integrates diode lasers driven by an electrical driver developed for very short high power pulses. It uses specifically developed highly efficient diode stacks with high frequency repetition rate up to 10 kHz, emitting at 800nm wavelength. The emitted beam is collimated and shaped with compact micro optics beam shaping system delivering a homogenized rectangular laser beam intensity distribution. The laser block is integrated with an ultrasound transducer in an ergonomically designed handset probe. This handset is a building block enabling for a low cost high frame rate photoacoustic and ultrasound imaging system. The probe was used with a modified ultrasound scanner and was tested by imaging a tissue mimicking phantom.

Numerical modelling as a cost-reduction tool for probability of detection of bolt hole eddy current testing

NASA Astrophysics Data System (ADS)

Mandache, C.; Khan, M.; Fahr, A.; Yanishevsky, M.

2011-03-01

Probability of detection (PoD) studies are broadly used to determine the reliability of specific nondestructive inspection procedures, as well as to provide data for damage tolerance life estimations and calculation of inspection intervals for critical components. They require inspections on a large set of samples, a fact that makes these statistical assessments time- and cost-consuming. Physics-based numerical simulations of nondestructive testing inspections could be used as a cost-effective alternative to empirical investigations. They realistically predict the inspection outputs as functions of the input characteristics related to the test piece, transducer and instrument settings, which are subsequently used to partially substitute and/or complement inspection data in PoD analysis. This work focuses on the numerical modelling aspects of eddy current testing for the bolt hole inspections of wing box structures typical of the Lockheed Martin C-130 Hercules and P-3 Orion aircraft, found in the air force inventory of many countries. Boundary element-based numerical modelling software was employed to predict the eddy current signal responses when varying inspection parameters related to probe characteristics, crack geometry and test piece properties. Two demonstrator exercises were used for eddy current signal prediction when lowering the driver probe frequency and changing the material's electrical conductivity, followed by subsequent discussions and examination of the implications on using simulated data in the PoD analysis. Despite some simplifying assumptions, the modelled eddy current signals were found to provide similar results to the actual inspections. It is concluded that physics-based numerical simulations have the potential to partially substitute or complement inspection data required for PoD studies, reducing the cost, time, effort and resources necessary for a full empirical PoD assessment.

SNAP: Small Next-generation Atmospheric Probe Concept

NASA Astrophysics Data System (ADS)

Sayanagi, K. M.; Dillman, R. A.; Atkinson, D. H.; Li, J.; Saikia, S.; Simon, A. A.; Spilker, T. R.; Wong, M. H.; Hope, D.

2017-12-01

We present a concept for a small, atmospheric probe that could be flexibly added to future missions that orbit or fly-by a giant planet as a secondary payload, which we call the Small Next-generation Atmospheric Probe (SNAP). SNAP's main scientific objectives are to determine the vertical distribution of clouds and cloud-forming chemical species, thermal stratification, and wind speed as a function of depth. As a case study, we present the advantages, cost and risk of adding SNAP to the future Uranus Orbiter and Probe flagship mission; in combination with the mission's main probe, SNAP would perform atmospheric in-situ measurements at a second location, and thus enable and enhance the scientific objectives recommended by the 2013 Planetary Science Decadal Survey and the 2014 NASA Science Plan to determine atmospheric spatial variabilities. We envision that the science objectives can be achieved with a 30-kg entry probe 0.5m in diameter (less than half the size of the Galileo probe) that reaches 5-bar pressure-altitude and returns data to Earth via the carrier spacecraft. As the baseline instruments, the probe will carry an Atmospheric Structure Instrument (ASI) that measures the temperature, pressure and acceleration, a carbon nanotube-based NanoChem atmospheric composition sensor, and an Ultra-Stable Oscillator (USO) to conduct a Doppler Wind Experiment (DWE). We also catalog promising technologies currently under development that will strengthen small atmospheric entry probe missions in the future. While SNAP is applicable to multiple planets, we examine the feasibility, benefits and impacts of adding SNAP to the Uranus Orbiter and Probe flagship mission. Our project is supported by NASA PSDS3 grant NNX17AK31G.

Highly specific noninvasive photoacoustic and positron emission tomography of brain plaque with functionalized croconium dye labeled by a radiotracer† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c6sc04798j Click here for additional data file.

PubMed Central

Liu, Yajing; Yang, Yanping; Sun, Mingjian; Cui, Mengchao; Fu, Ying; Lin, Yu

2017-01-01

Highly-efficient targeting probes are desirable for disease diagnosis and functional imaging. However, most of the current near-infrared (NIR) probes suffer from low signal conversion, insufficient photostability, poor probe specificity, and limited functions. Herein, an NIR ultrahigh absorbing croconium dye for amyloid (CDA) was designed and synthesized to specifically bind to cerebrovascular amyloid without antibody linkage. This unique CDA is able to strongly bind the hydrophobic channels of amyloid beta (Aβ) fiber with a very strong binding energy of –9.3 kcal mol–1. Our experimental results demonstrate that the amphipathic dye with an intense absorption peak at 800 nm generated a significant local temperature surge under low-power laser irradiation. Compared with representative prominent indocyanine green, Prussian blue, and gold nanorods, this probe can produce the strongest photoacoustic signal based on the same mass concentration. Labeled with radioactive 18F, this multifunctional probe allowed for the ultrasensitive photoacoustic tomography (PAT)/positron emission tomography (PET)/fluorescence imaging of Aβ plaques in the brain cortex. Featured with high spatial resolution and optical specificity, PAT was intrinsically suitable for imaging pathological sites on cortical vessels, whereas PET revealed whole-body anatomy with quantitative biodistribution information. Our study shows that a CDA-based functionalized dye aided with PAT and PET is capable of plaque diagnosis and localization. PMID:28451353

Improved analysis techniques for cylindrical and spherical double probes.

PubMed

Beal, Brian; Johnson, Lee; Brown, Daniel; Blakely, Joseph; Bromaghim, Daron

2012-07-01

A versatile double Langmuir probe technique has been developed by incorporating analytical fits to Laframboise's numerical results for ion current collection by biased electrodes of various sizes relative to the local electron Debye length. Application of these fits to the double probe circuit has produced a set of coupled equations that express the potential of each electrode relative to the plasma potential as well as the resulting probe current as a function of applied probe voltage. These equations can be readily solved via standard numerical techniques in order to determine electron temperature and plasma density from probe current and voltage measurements. Because this method self-consistently accounts for the effects of sheath expansion, it can be readily applied to plasmas with a wide range of densities and low ion temperature (T(i)/T(e) ≪ 1) without requiring probe dimensions to be asymptotically large or small with respect to the electron Debye length. The presented approach has been successfully applied to experimental measurements obtained in the plume of a low-power Hall thruster, which produced a quasineutral, flowing xenon plasma during operation at 200 W on xenon. The measured plasma densities and electron temperatures were in the range of 1 × 10(12)-1 × 10(17) m(-3) and 0.5-5.0 eV, respectively. The estimated measurement uncertainty is +6%∕-34% in density and +∕-30% in electron temperature.

Eddy current proximity measurement of perpendicular tubes from within pressure tubes in CANDU nuclear reactors

NASA Astrophysics Data System (ADS)

Bennett, P. F. D.; Underhill, P. R.; Morelli, J.; Krause, T. W.

2018-04-01

Fuel channels in CANDU® (CANada Deuterium Uranium) nuclear reactors consist of two non-concentric tubes; an inner pressure tube (PT) and a larger diameter calandria tube (CT). Up to 400 horizontally mounted fuel channels are contained within a calandria vessel, which also holds the heavy water moderator. Certain fuel channels pass perpendicularly over horizontally oriented tubes (nozzles) that are part of the reactor's liquid injection shutdown system (LISS). Due to sag, these fuel channels are at risk of coming into contact with the LISS nozzles. In the event of contact between the LISS nozzle and CT, flow-induced vibrations from within the moderator could lead to fretting and deformation of the CT. LISS nozzle proximity to CTs is currently measured optically from within the calandria vessel, but from outside the fuel channels. Measurement by an independent means would provide confidence in optical results and supplement cases where optical observations are not possible. Separation of PT and CT, known as gap, is monitored from within the PT using a transmit-receive eddy current probe. Investigation of the eddy current based gap probe as a tool to also measure proximity of LISS nozzles was carried out experimentally in this work. Eddy current response as a function of LISS-PT proximity was recorded. When PT-CT gap, PT wall thickness, PT resistivity and probe lift-off variations were not present this dependence could be used to determine the LISS-PT proximity. This method has the potential to provide LISS-CT proximity using existing gap measurement data. Obtaining LISS nozzle proximity at multiple inspection intervals could be used to provide an estimate of the time to LISS-CT contact, and thereby provide a means of optimizing maintenance schedules.

Hollow Cathode and Keeper-region Plasma Measurements Using Ultra-fast Miniature Scanning Probes

NASA Technical Reports Server (NTRS)

Goebel, Dan M.; Jameson, Kristina K.; Watkins, Ron M.; Katz, Ira

2004-01-01

In order to support the development of comprehensive performance and life models for future deep space missions that will utilize ion thrusters, we have undertaken a study of the plasma structure in hollow cathodes using an new pneumatic scanning probe diagnostic. This device is designed to insert a miniature probe directly into the hollow cathode orifice from either the upstream insert region in the interior of the hollow cathode, or from the downstream keeper-plasma region at the exit of the hollow cathode, to provide complete axial profiles of the discharge plasma parameters. Previous attempts to diagnose this region with probes was Limited by the melting of small probes in the intense discharge near the orifice, or caused significant perturbation of the plasma by probes large enough to survive. Our new probe is extremely compact, and when configured as a single Langmuir probe, the ceramic tube insulator is only 0.5mm in diameter and the current collecting conductor has a total area of 0.002 cm2. A series of current-voltage characteristics are obtained by applying a rapid sawtooth voltage waveform to the probe as it is scanned by the pneumatic actuator into and out of the plasma region, The bellow-sealed pneumatic drive scans the probe 4 cm in the cathode insert region and 10 cm in the anode/keeper plasmas region at average speeds of about 1 mm/msec, and the residence time at the end of the insertion stroke in the densest part of the plasma near the orifice is measured to be only 10 msec. Since the voltage sweep time is fast compared to the motion of the probe, axial profiles of the plasma density, temperature and potential with reasonable spatial resolution are obtained. Measurements of the internal cathode pressures and the axial plasma-parameter profiles for a hollow cathode operating at discharge currents of up to 35 A in xenon will be presented.

Pulsed eddy current differential probe to detect the defects in a stainless steel pipe

NASA Astrophysics Data System (ADS)

Angani, C. S.; Park, D. G.; Kim, C. G.; Leela, P.; Kishore, M.; Cheong, Y. M.

2011-04-01

Pulsed eddy current (PEC) is an electromagnetic nondestructive technique widely used to detect and quantify the flaws in conducting materials. In the present study a differential Hall-sensor probe which is used in the PEC system has been fabricated for the detection of defects in stainless steel pipelines. The differential probe has an exciting coil with two Hall-sensors. A stainless steel test sample with electrical discharge machining (EDM) notches under different depths of 1-5 mm was made and the sample was laminated by plastic insulation having uniform thickness to simulate the pipelines in nuclear power plants (NPPs). The driving coil in the probe is excited by a rectangular current pulse and the resultant response, which is the difference of the two Hall-sensors, has been detected as the PEC probe signal. The discriminating time domain features of the detected pulse such as peak value and time to zero are used to interpret the experimental results with the defects in the test sample. A feature extraction technique such as spectral power density has been devised to infer the PEC response.

Standard Practices for Usage of Inductive Magnetic Field Probes with Application to Electric Propulsion Testing

NASA Technical Reports Server (NTRS)

Polzin, Kurt A.; Hill, Carrie S.

2013-01-01

Inductive magnetic field probes (also known as B-dot probes and sometimes as B-probes or magnetic probes) are useful for performing measurements in electric space thrusters and various plasma accelerator applications where a time-varying magnetic field is present. Magnetic field probes have proven to be a mainstay in diagnosing plasma thrusters where changes occur rapidly with respect to time, providing the means to measure the magnetic fields produced by time-varying currents and even an indirect measure of the plasma current density through the application of Ampère's law. Examples of applications where this measurement technique has been employed include pulsed plasma thrusters and quasi-steady magnetoplasmadynamic thrusters. The Electric Propulsion Technical Committee (EPTC) of the American Institute of Aeronautics and Astronautics (AIAA) was asked to assemble a Committee on Standards (CoS) for Electric Propulsion Testing. The assembled CoS was tasked with developing Standards and Recommended Practices for various diagnostic techniques used in the evaluation of plasma thrusters. These include measurements that can yield either global information related to a thruster and its performance or detailed, local data related to the specific physical processes occurring in the plasma. This paper presents a summary of the standard, describing the preferred methods for fabrication, calibration, and usage of inductive magnetic field probes for use in diagnosing plasma thrusters. Inductive magnetic field probes (also called B-dot probes throughout this document) are commonly used in electric propulsion (EP) research and testing to measure unsteady magnetic fields produced by time-varying currents. The B-dot probe is relatively simple in construction, and requires minimal cost, making it a low-cost technique that is readily accessible to most researchers. While relatively simple, the design of a B-dot probe is not trivial and there are many opportunities for errors in probe construction, calibration, and usage, and in the post-processing of data that is produced by the probe. There are typically several ways in which each of these steps can be approached, and different applications may require more or less vigorous attention to various issues.

Flexible Cryogenic Temperature and Liquid-Level Probes

NASA Technical Reports Server (NTRS)

Haberbusch, Mark

2005-01-01

Lightweight, flexible probes have been developed for measuring temperatures at multiple locations in tanks that contain possibly pressurized cryogenic fluids. If the fluid in a given tank is subcritical (that is, if it consists of a liquid and its vapor), then in one of two modes of operation, the temperature measurements made by a probe of this type can be used to deduce the approximate level of the liquid. The temperature sensors are silicon diodes located at intervals along a probe. If the probe is to be used to measure a temperature gradient along a given axis in the tank, then the probe must be mounted along that axis. In the temperature-measurement mode, a constant small electric current is applied to each diode and the voltage across the diode a known function of the current and temperature is measured as an indication of its temperature. For the purpose of this measurement, small electric current signifies a current that is not large enough to cause a significant increase in the measured temperature. More specifically, the probe design calls for a current of 10 A, which, in the cryogenic temperature range of interest, generates heat at a rate of only about 0.01 mW per diode. In the liquid-level-sensing mode, one applies a larger current (30 mA) to each diode so as to heat each diode appreciably (with a power of about 36 mW in the temperature range of interest). Because the liquid cools the diode faster than does the vapor, the temperature of the diode is less when the diode is immersed in the liquid than when it is above the surface of the liquid. Thus, the temperature (voltage) reading from each diode can be used to determine whether the liquid level is above or below the diode, and one can deduce that the liquid level lies between two adjacent diodes, the lower one of which reads a significantly lower temperature. The aforementioned techniques for measuring temperature and deducing liquid level are not new. What is new here are the designs of the probes and of associated external electronic circuitry. In each probe, the diodes and the lead wires are embedded in a strong, lightweight, flexible polyimide strip. Each probe is constructed as an integral unit that includes a multipin input/output plug or socket for solderless connection of the lead wires to the external circuitry. The polyimide strip includes mounting tabs with holes that can accommodate rivets, screws, or other fasteners. Alternatively, a probe can be mounted by use of an epoxy. A probe can be manufactured to almost any length or width, and the diodes can be embedded at almost any desired location along and across the polyimide strip. In designing a probe for a specific application, one seeks a compromise between (1) minimizing the number of diodes in order to minimize the complexity of input/output connections and external electronic circuitry while (2) using enough diodes to obtain the required precision. Optionally, to minimize spurious heating of the cryogenic fluid, the external circuitry can be designed to apply power to the probe only during brief measurement intervals. Assuming that the external circuitry is maintained at a steady temperature, a power-on interval of only a few seconds is sufficient to obtain accurate data on temperatures and/or the height of the liquid/vapor interface.

Flexible Cryogenic Temperature and Liquid-Level Probes

NASA Technical Reports Server (NTRS)

Haberbusch, Mark

2003-01-01

Lightweight, flexible probes have been developed for measuring temperatures at multiple locations in tanks that contain possibly pressurized cryogenic fluids. If the fluid in a given tank is subcritical (that is, if it consists of a liquid and its vapor), then in one of two modes of operation, the temperature measurements made by a probe of this type can be used to deduce the approximate level of the liquid. The temperature sensors are silicon diodes located at intervals along a probe. If the probe is to be used to measure a temperature gradient along a given axis in the tank, then the probe must be mounted along that axis. In the non-liquid-level-sensing temperature-measurement mode, a constant small electric current is applied to each diode and the voltage across the diode . a known function of the current and temperature . is measured as an indication of its temperature. For the purpose of this measurement, "small electric current" signifies a current that is not large enough to cause a significant increase in the measured temperature. More specifically, the probe design calls for a current of 10 A, which, in the cryogenic temperature range of interest, generates heat at a rate of only about 0.01 mW per diode. In the liquid-level-sensing mode, one applies a larger current (30 mA) to each diode so as to heat each diode appreciably (with a power of about 36 mW in the temperature range of interest). Because the liquid cools the diode faster than does the vapor, the temperature of the diode is less when diode is immersed in the liquid than when it is above the surface of the liquid. Thus, the temperature (voltage) reading from each diode can be used to determine whether the liquid level is above or below the diode, and one can deduce that the liquid level lies between two adjacent diodes, the lower one of which reads a significantly lower temperature. The aforementioned techniques for measuring temperature and deducing liquid level are not new. What is new here are the designs of the probes and of associated external electronic circuitry. In each probe, the diodes and the lead wires are embedded in a strong, lightweight, flexible polyimide strip. Each probe is constructed as an integral unit that includes a multipin input/output plug or socket for solderless connection of the lead wires to the external circuitry. The polyimide strip includes mounting tabs with holes that can accommodate rivets, screws, or other fasteners. Alternatively, a probe can be mounted by use of an epoxy. A probe can be manufactured to almost any length or width, and the diodes can be embedded at almost any desired locations along and across the polyimide strip. In designing a probe for a specific application, one seeks a compromise between (1) minimizing the number of diodes in order to minimize the complexity of input/output connections and external electronic circuitry while (2) using enough diodes to obtain the required precision. Optionally, to minimize spurious heating of the cryogenic fluid, the external circuitry can be designed to apply power to the probe only during brief measurement intervals. Assuming that the external circuitry is maintained at a steady temperature, a power-on interval of only a few seconds is sufficient to obtain accurate data on temperatures and/or the height of the liquid/vapor interface.

Measuring the lateral charge-carrier mobility in metal-insulator-semiconductor capacitors via Kelvin-probe.

PubMed

Milotti, Valeria; Pietsch, Manuel; Strunk, Karl-Philipp; Melzer, Christian

2018-01-01

We report a Kelvin-probe method to investigate the lateral charge-transport properties of semiconductors, most notably the charge-carrier mobility. The method is based on successive charging and discharging of a pre-biased metal-insulator-semiconductor stack by an alternating voltage applied to one edge of a laterally confined semiconductor layer. The charge carriers spreading along the insulator-semiconductor interface are directly measured by a Kelvin-probe, following the time evolution of the surface potential. A model is presented, describing the device response for arbitrary applied biases allowing the extraction of the lateral charge-carrier mobility from experimentally measured surface potentials. The method is tested using the organic semiconductor poly(3-hexylthiophene), and the extracted mobilities are validated through current voltage measurements on respective field-effect transistors. Our widely applicable approach enables robust measurements of the lateral charge-carrier mobility in semiconductors with weak impact from the utilized contact materials.

Measuring the lateral charge-carrier mobility in metal-insulator-semiconductor capacitors via Kelvin-probe

NASA Astrophysics Data System (ADS)

Milotti, Valeria; Pietsch, Manuel; Strunk, Karl-Philipp; Melzer, Christian

2018-01-01

We report a Kelvin-probe method to investigate the lateral charge-transport properties of semiconductors, most notably the charge-carrier mobility. The method is based on successive charging and discharging of a pre-biased metal-insulator-semiconductor stack by an alternating voltage applied to one edge of a laterally confined semiconductor layer. The charge carriers spreading along the insulator-semiconductor interface are directly measured by a Kelvin-probe, following the time evolution of the surface potential. A model is presented, describing the device response for arbitrary applied biases allowing the extraction of the lateral charge-carrier mobility from experimentally measured surface potentials. The method is tested using the organic semiconductor poly(3-hexylthiophene), and the extracted mobilities are validated through current voltage measurements on respective field-effect transistors. Our widely applicable approach enables robust measurements of the lateral charge-carrier mobility in semiconductors with weak impact from the utilized contact materials.

Detection of single-copy functional genes in prokaryotic cells by two-pass TSA-FISH with polynucleotide probes.

PubMed

Kawakami, Shuji; Hasegawa, Takuya; Imachi, Hiroyuki; Yamaguchi, Takashi; Harada, Hideki; Ohashi, Akiyoshi; Kubota, Kengo

2012-02-01

In situ detection of functional genes with single-cell resolution is currently of interest to microbiologists. Here, we developed a two-pass tyramide signal amplification (TSA)-fluorescence in situ hybridization (FISH) protocol with PCR-derived polynucleotide probes for the detection of single-copy genes in prokaryotic cells. The mcrA gene and the apsA gene in methanogens and sulfate-reducing bacteria, respectively, were targeted. The protocol showed bright fluorescence with a good signal-to-noise ratio and achieved a high efficiency of detection (>98%). The discrimination threshold was approximately 82-89% sequence identity. Microorganisms possessing the mcrA or apsA gene in anaerobic sludge samples were successfully detected by two-pass TSA-FISH with polynucleotide probes. The developed protocol is useful for identifying single microbial cells based on functional gene sequences. Copyright © 2011 Elsevier B.V. All rights reserved.

Plant cell wall characterization using scanning probe microscopy techniques

PubMed Central

Yarbrough, John M; Himmel, Michael E; Ding, Shi-You

2009-01-01

Lignocellulosic biomass is today considered a promising renewable resource for bioenergy production. A combined chemical and biological process is currently under consideration for the conversion of polysaccharides from plant cell wall materials, mainly cellulose and hemicelluloses, to simple sugars that can be fermented to biofuels. Native plant cellulose forms nanometer-scale microfibrils that are embedded in a polymeric network of hemicelluloses, pectins, and lignins; this explains, in part, the recalcitrance of biomass to deconstruction. The chemical and structural characteristics of these plant cell wall constituents remain largely unknown today. Scanning probe microscopy techniques, particularly atomic force microscopy and its application in characterizing plant cell wall structure, are reviewed here. We also further discuss future developments based on scanning probe microscopy techniques that combine linear and nonlinear optical techniques to characterize plant cell wall nanometer-scale structures, specifically apertureless near-field scanning optical microscopy and coherent anti-Stokes Raman scattering microscopy. PMID:19703302

Sub-picowatt/kelvin resistive thermometry for probing nanoscale thermal transport.

PubMed

Zheng, Jianlin; Wingert, Matthew C; Dechaumphai, Edward; Chen, Renkun

2013-11-01

Advanced instrumentation in thermometry holds the key for experimentally probing fundamental heat transfer physics. However, instrumentation with simultaneously high thermometry resolution and low parasitic heat conduction is still not available today. Here we report a resistive thermometry scheme with ~50 μK temperature resolution and ~0.25 pW/K thermal conductance resolution, which is achieved through schemes using both modulated heating and common mode noise rejection. The suspended devices used herein have been specifically designed to possess short thermal time constants and minimal attenuation effects associated with the modulated heating current. Furthermore, we have systematically characterized the parasitic background heat conductance, which is shown to be significantly reduced using the new device design and can be effectively eliminated using a "canceling" scheme. Our results pave the way for probing fundamental nanoscale thermal transport processes using a general scheme based on resistive thermometry.

Electrical Resistance Based Damage Modeling of Multifunctional Carbon Fiber Reinforced Polymer Matrix Composites

NASA Astrophysics Data System (ADS)

Hart, Robert James

In the current thesis, the 4-probe electrical resistance of carbon fiber-reinforced polymer (CFRP) composites is utilized as a metric for sensing low-velocity impact damage. A robust method has been developed for recovering the directionally dependent electrical resistivities using an experimental line-type 4-probe resistance method. Next, the concept of effective conducting thickness was uniquely applied in the development of a brand new point-type 4-probe method for applications with electrically anisotropic materials. An extensive experimental study was completed to characterize the 4-probe electrical resistance of CFRP specimens using both the traditional line-type and new point-type methods. Leveraging the concept of effective conducting thickness, a novel method was developed for building 4-probe electrical finite element (FE) models in COMSOL. The electrical models were validated against experimental resistance measurements and the FE models demonstrated predictive capabilities when applied to CFRP specimens with varying thickness and layup. These new models demonstrated a significant improvement in accuracy compared to previous literature and could provide a framework for future advancements in FE modeling of electrically anisotropic materials. FE models were then developed in ABAQUS for evaluating the influence of prescribed localized damage on the 4-probe resistance. Experimental data was compiled on the impact response of various CFRP laminates, and was used in the development of quasi- static FE models for predicting presence of impact-induced delamination. The simulation-based delamination predictions were then integrated into the electrical FE models for the purpose of studying the influence of realistic damage patterns on electrical resistance. When the size of the delamination damage was moderate compared to the electrode spacing, the electrical resistance increased by less than 1% due to the delamination damage. However, for a specimen with large delamination extending beyond the electrode locations, the oblique resistance increased by 30%. This result suggests that for damage sensing applications, the spacing of electrodes relative to the size of the delamination is important. Finally CT image data was used to model 3-D void distributions and the electrical response of such specimens were compared to models with no voids. As the void content increased, the electrical resistance increased non-linearly. The relationship between void content and electrical resistance was attributed to a combination of three factors: (i) size and shape, (ii) orientation, and (iii) distribution of voids. As a whole, the current thesis provides a comprehensive framework for developing predictive, resistance-based damage sensing models for CFRP laminates of various layup and thickness.

Surfing Silicon Nanofacets for Cold Cathode Electron Emission Sites.

PubMed

Basu, Tanmoy; Kumar, Mohit; Saini, Mahesh; Ghatak, Jay; Satpati, Biswarup; Som, Tapobrata

2017-11-08

Point sources exhibit low threshold electron emission due to local field enhancement at the tip. In the case of silicon, however, the realization of tip emitters has been hampered by unwanted oxidation, limiting the number of emission sites and the overall current. In contrast to this, here, we report the fascinating low threshold (∼0.67 V μm -1 ) cold cathode electron emission from silicon nanofacets (Si-NFs). The ensembles of nanofacets fabricated at different time scales, under low energy ion impacts, yield tunable field emission with a Fowler-Nordheim tunneling field in the range of 0.67-4.75 V μm -1 . The local probe surface microscopy-based tunneling current mapping in conjunction with Kelvin probe force microscopy measurements revealed that the valleys and a part of the sidewalls of the nanofacets contribute more to the field emission process. The observed lowest turn-on field is attributed to the absence of native oxide on the sidewalls of the smallest facets as well as their lowest work function. In addition, first-principle density functional theory-based simulation revealed a crystal orientation-dependent work function of Si, which corroborates well with our experimental observations. The present study demonstrates a novel way to address the origin of the cold cathode electron emission sites from Si-NFs fabricated at room temperature. In principle, the present methodology can be extended to probe the cold cathode electron emission sites from any nanostructured material.

Single-Step qPCR and dPCR Detection of Diverse CRISPR-Cas9 Gene Editing Events In Vivo.

PubMed

Falabella, Micol; Sun, Linqing; Barr, Justin; Pena, Andressa Z; Kershaw, Erin E; Gingras, Sebastien; Goncharova, Elena A; Kaufman, Brett A

2017-10-05

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-associated protein 9 (Cas9)-based technology is currently the most flexible means to create targeted mutations by recombination or indel mutations by nonhomologous end joining. During mouse transgenesis, recombinant and indel alleles are often pursued simultaneously. Multiple alleles can be formed in each animal to create significant genetic complexity that complicates the CRISPR-Cas9 approach and analysis. Currently, there are no rapid methods to measure the extent of on-site editing with broad mutation sensitivity. In this study, we demonstrate the allelic diversity arising from targeted CRISPR editing in founder mice. Using this DNA sample collection, we validated specific quantitative and digital PCR methods (qPCR and dPCR, respectively) for measuring the frequency of on-target editing in founder mice. We found that locked nucleic acid (LNA) probes combined with an internal reference probe (Drop-Off Assay) provide accurate measurements of editing rates. The Drop-Off LNA Assay also detected on-target CRISPR-Cas9 gene editing in blastocysts with a sensitivity comparable to PCR-clone sequencing. Lastly, we demonstrate that the allele-specific LNA probes used in qPCR competitor assays can accurately detect recombinant mutations in founder mice. In summary, we show that LNA-based qPCR and dPCR assays provide a rapid method for quantifying the extent of on-target genome editing in vivo , testing RNA guides, and detecting recombinant mutations. Copyright © 2017 Falabella et al.

PSMA-Targeted Nano-Conjugates as Dual-Modality (MRI/PET) Imaging Probes for the Non-Invasive Detection of Prostate Cancer

DTIC Science & Technology

2009-10-01

be made. Currently, iodine based compounds are used to enhance contrast of CT which have the limitations of short imaging window due to rapid...number compared to conventionally used iodine compounds . Nanoparticle based CT contrast agents have been demonstrated for vascular imaging, which...constructs with gamma or positron emitting isotopes through a covalent attachment of a bifunctional chelator to the nanoparticles surface. However, in

Detection of Cracks at Welds in Steel Tubing Using Flux Focusing Electromagnetic Probe

NASA Technical Reports Server (NTRS)

Wincheski, Buzz; Fulton, Jim; Nath, Shridhar; Simpson, John; Namkung, Min

1994-01-01

The inspection of weldments in critical pressure vessel joints is a major concern in the nuclear power industry. Corrosive environments can speed the fatigue process and access to the critical area is often limited. Eddy current techniques have begun to be used to help overcome these obstacles [1]. As direct contact and couplants are not required, remote areas can be inspected by simply snaking an eddy current coil into the intake tube of the vessel. The drawback of the eddy current method has been the high sensitivity to small changes in the conductivity and permeability of the test piece which are known to vary at weldments [1]. The flaw detection mechanism of the flux focusing electromagnetic probe can help alleviate these difficulties and provide a unique capability for detecting longitudinal fatigue cracks in critical tube structures. The Flux Focusing Electromagnetic Flaw Detector, originally invented for the detection of fatigue and corrosion damage in aluminum plates [2-3], has been adapted for use in testing steel tubing for longitudinal fatigue cracks. The modified design allows for the probe to be placed axisymmetrically into the tubing, inducing eddy currents in the tube wall. The pickup coil of the probe is fixed slightly below the primary windings and is rotated 90 so that its axis is normal to the tube wall. The magnetic flux of the primary coil is focused through the use of ferromagnetic material so that in the absence of fatigue damage there will be no flux linkage with the pickup coil. The presence of a longitudinal fatigue crack will cause the eddy currents induced in the tube wall to flow around the flaw and directly under the pickup coil. The magnetic field associated with these currents will then link the pickup coil and an unambiguous increase in the output voltage of the probe will be measured. The use of the flux focusing electromagnetic probe is especially suited for the detection of flaws originating at or near tube welds. The probe is shown to discriminate against signals due solely to the weld joint so that flaw signals are not hidden in the background in these locations. Experimental and finite element modeling results are presented for the flaw detection capabilities of the probe in stainless steel tubes.

Three-dimensional fluorescence-enhanced optical tomography using a hand-held probe based imaging system

PubMed Central

Ge, Jiajia; Zhu, Banghe; Regalado, Steven; Godavarty, Anuradha

2008-01-01

Hand-held based optical imaging systems are a recent development towards diagnostic imaging of breast cancer. To date, all the hand-held based optical imagers are used to perform only surface mapping and target localization, but are not capable of demonstrating tomographic imaging. Herein, a novel hand-held probe based optical imager is developed towards three-dimensional (3-D) optical tomography studies. The unique features of this optical imager, which primarily consists of a hand-held probe and an intensified charge coupled device detector, are its ability to; (i) image large tissue areas (5×10 sq. cm) in a single scan, (ii) perform simultaneous multiple point illumination and collection, thus reducing the overall imaging time; and (iii) adapt to varying tissue curvatures, from a flexible probe head design. Experimental studies are performed in the frequency domain on large slab phantoms (∼650 ml) using fluorescence target(s) under perfect uptake (1:0) contrast ratios, and varying target depths (1–2 cm) and X-Y locations. The effect of implementing simultaneous over sequential multiple point illumination towards 3-D tomography is experimentally demonstrated. The feasibility of 3-D optical tomography studies has been demonstrated for the first time using a hand-held based optical imager. Preliminary fluorescence-enhanced optical tomography studies are able to reconstruct 0.45 ml target(s) located at different target depths (1–2 cm). However, the depth recovery was limited as the actual target depth increased, since only reflectance measurements were acquired. Extensive tomography studies are currently carried out to determine the resolution and performance limits of the imager on flat and curved phantoms. PMID:18697559

Three-dimensional fluorescence-enhanced optical tomography using a hand-held probe based imaging system.

PubMed

Ge, Jiajia; Zhu, Banghe; Regalado, Steven; Godavarty, Anuradha

2008-07-01

Hand-held based optical imaging systems are a recent development towards diagnostic imaging of breast cancer. To date, all the hand-held based optical imagers are used to perform only surface mapping and target localization, but are not capable of demonstrating tomographic imaging. Herein, a novel hand-held probe based optical imager is developed towards three-dimensional (3-D) optical tomography studies. The unique features of this optical imager, which primarily consists of a hand-held probe and an intensified charge coupled device detector, are its ability to; (i) image large tissue areas (5 x 10 sq. cm) in a single scan, (ii) perform simultaneous multiple point illumination and collection, thus reducing the overall imaging time; and (iii) adapt to varying tissue curvatures, from a flexible probe head design. Experimental studies are performed in the frequency domain on large slab phantoms (approximately 650 ml) using fluorescence target(s) under perfect uptake (1:0) contrast ratios, and varying target depths (1-2 cm) and X-Y locations. The effect of implementing simultaneous over sequential multiple point illumination towards 3-D tomography is experimentally demonstrated. The feasibility of 3-D optical tomography studies has been demonstrated for the first time using a hand-held based optical imager. Preliminary fluorescence-enhanced optical tomography studies are able to reconstruct 0.45 ml target(s) located at different target depths (1-2 cm). However, the depth recovery was limited as the actual target depth increased, since only reflectance measurements were acquired. Extensive tomography studies are currently carried out to determine the resolution and performance limits of the imager on flat and curved phantoms.

Quality control of the soil moisture probe response patterns from a green infrastructure site using Dynamic Time Warping (DTW) and association rule learning

NASA Astrophysics Data System (ADS)

Yu, Z.; Bedig, A.; Quigley, M.; Montalto, F. A.

2017-12-01

In-situ field monitoring can help to improve the design and management of decentralized Green Infrastructure (GI) systems in urban areas. Because of the vast quantity of continuous data generated from multi-site sensor systems, cost-effective post-construction opportunities for real-time control are limited; and the physical processes that influence the observed phenomena (e.g. soil moisture) are hard to track and control. To derive knowledge efficiently from real-time monitoring data, there is currently a need to develop more efficient approaches to data quality control. In this paper, we employ dynamic time warping method to compare the similarity of two soil moisture patterns without ignoring the inherent autocorrelation. We also use a rule-based machine learning method to investigate the feasibility of detecting anomalous responses from soil moisture probes. The data was generated from both individual and clusters of probes, deployed in a GI site in Milwaukee, WI. In contrast to traditional QAQC methods, which seek to detect outliers at individual time steps, the new method presented here converts the continuous time series into event-based symbolic sequences from which unusual response patterns can be detected. Different Matching rules are developed on different physical characteristics for different seasons. The results suggest that this method could be used alternatively to detect sensor failure, to identify extreme events, and to call out abnormal change patterns, compared to intra-probe and inter-probe historical observations. Though this algorithm was developed for soil moisture probes, the same approach could easily be extended to advance QAQC efficiency for any continuous environmental datasets.

Redox polymer and probe DNA tethered to gold electrodes for enzyme-amplified amperometric detection of DNA hybridization.

PubMed

Kavanagh, Paul; Leech, Dónal

2006-04-15

The detection of nucleic acids based upon recognition surfaces formed by co-immobilization of a redox polymer mediator and DNA probe sequences on gold electrodes is described. The recognition surface consists of a redox polymer, [Os(2,2'-bipyridine)2(polyvinylimidazole)(10)Cl](+/2+), and a model single DNA strand cross-linked and tethered to a gold electrode via an anchoring self-assembled monolayer (SAM) of cysteamine. Hybridization between the immobilized probe DNA of the recognition surface and a biotin-conjugated target DNA sequence (designed from the ssrA gene of Listeria monocytogenes), followed by addition of an enzyme (glucose oxidase)-avidin conjugate, results in electrical contact between the enzyme and the mediating redox polymer. In the presence of glucose, the current generated due to the catalytic oxidation of glucose to gluconolactone is measured, and a response is obtained that is binding-dependent. The tethering of the probe DNA and redox polymer to the SAM improves the stability of the surface to assay conditions of rigorous washing and high salt concentration (1 M). These conditions eliminate nonspecific interaction of both the target DNA and the enzyme-avidin conjugate with the recognition surfaces. The sensor response increases linearly with increasing concentration of target DNA in the range of 1 x 10(-9) to 2 x 10(-6) M. The detection limit is approximately 1.4 fmol, (corresponding to 0.2 nM of target DNA). Regeneration of the recognition surface is possible by treatment with 0.25 M NaOH solution. After rehybridization of the regenerated surface with the target DNA sequence, >95% of the current is recovered, indicating that the redox polymer and probe DNA are strongly bound to the surface. These results demonstrate the utility of the proposed approach.

Belief Inhibition in Children's Reasoning: Memory-Based Evidence

ERIC Educational Resources Information Center

Steegen, Sara; Neys, Wim De

2012-01-01

Adult reasoning has been shown as mediated by the inhibition of intuitive beliefs that are in conflict with logic. The current study introduces a classic procedure from the memory field to investigate belief inhibition in 12- to 17-year-old reasoners. A lexical decision task was used to probe the memory accessibility of beliefs that were cued…

Plasma contactor research - 1991

NASA Technical Reports Server (NTRS)

Buchholtz, Brett; Williams, John D.; Wilbur, Paul J.

1992-01-01

A report describing the operating principles of hollow-cathode-based plasma contactors emitting or collecting electrons from an ambient plasma is summarized. Preliminary experiments conducted to determine the noise generated by these plasma contactors in the emission-current return line and in the plasma near it are described. These noise data are measured as current fluctuations in the return line and to the Langmuir probe and then analyzed using a fast Fourier transform technique. The spectral compositions of the data are characterized using power spectral density plots which are examined to identify possible noise source(s) and production mechanism(s). The precautions taken in the construction and calibration of the instrumentation to assure adequate frequency response are described. Experimental results show that line-current noise levels are typically 2 percent of the electron current being emitted or collected. However, noise levels increase to as much as 20 percent of the electron current at a few electron-collection operating conditions. The frequencies associated with most of the noise were harmonics of the 60 Hz input to system power supplies. Plasma noise had characteristics similar in magnitude and frequency to those for the return-line noise, but they contained additional features at frequencies considered to be related to ion-acoustic instabilities. Also discussed is a new probe positioning system built to facilitate future plasma-contractor research.

Estimation of plasma ion saturation current and reduced tip arcing using Langmuir probe harmonics.

PubMed

Boedo, J A; Rudakov, D L

2017-03-01

We present a method to calculate the ion saturation current, I sat , for Langmuir probes at high frequency (>100 kHz) using the harmonics technique and we compare that to a direct measurement of I sat . It is noted that the I sat estimation can be made directly by the ratio of harmonic amplitudes, without explicitly calculating T e . We also demonstrate that since the probe tips using the harmonic method are oscillating near the floating potential, drawing little power, this method reduces tip heating and arcing and allows plasma density measurements at a plasma power flux that would cause continuously biased tips to arc. A multi-probe array is used, with two spatially separated tips employing the harmonics technique and measuring the amplitude of at least two harmonics per tip. A third tip, located between the other two, measures the ion saturation current directly. We compare the measured and calculated ion saturation currents for a variety of plasma conditions and demonstrate the validity of the technique and its use in reducing arcs.

Estimation of plasma ion saturation current and reduced tip arcing using Langmuir probe harmonics

DOE Office of Scientific and Technical Information (OSTI.GOV)

Boedo, J. A.; Rudakov, D. L.

Here we present a method to calculate the ion saturation current, I sat, for Langmuir probes at high frequency (>100 kHz) using the harmonics technique and we compare that to a direct measurement of I sat. It is noted that the Isat estimation can be made directly by the ratio of harmonic amplitudes, without explicitly calculating T e. We also demonstrate that since the probe tips using the harmonic method are oscillating near the floating potential, drawing little power, this method reduces tip heating and arcing and allows plasma density measurements at a plasma power flux that would cause continuouslymore » biased tips to arc. A multi-probe array is used, with two spatially separated tips employing the harmonics technique and measuring the amplitude of at least two harmonics per tip. A third tip, located between the other two, measures the ion saturation current directly. We compare the measured and calculated ion saturation currents for a variety of plasma conditions and demonstrate the validity of the technique and it’s use in reducing arcs.« less

Estimation of plasma ion saturation current and reduced tip arcing using Langmuir probe harmonics

DOE PAGES

Boedo, J. A.; Rudakov, D. L.

2017-03-20

Here we present a method to calculate the ion saturation current, I sat, for Langmuir probes at high frequency (>100 kHz) using the harmonics technique and we compare that to a direct measurement of I sat. It is noted that the Isat estimation can be made directly by the ratio of harmonic amplitudes, without explicitly calculating T e. We also demonstrate that since the probe tips using the harmonic method are oscillating near the floating potential, drawing little power, this method reduces tip heating and arcing and allows plasma density measurements at a plasma power flux that would cause continuouslymore » biased tips to arc. A multi-probe array is used, with two spatially separated tips employing the harmonics technique and measuring the amplitude of at least two harmonics per tip. A third tip, located between the other two, measures the ion saturation current directly. We compare the measured and calculated ion saturation currents for a variety of plasma conditions and demonstrate the validity of the technique and it’s use in reducing arcs.« less

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhao, H.; Li, X.; Baker, D. N.

Based on comprehensive measurements from Helium, Oxygen, Proton, and Electron Mass Spectrometer Ion Spectrometer, Relativistic Electron-Proton Telescope, and Radiation Belt Storm Probes Ion Composition Experiment instruments on the Van Allen Probes, comparative studies of ring current electrons and ions are performed and the role of energetic electrons in the ring current dynamics is investigated. The deep injections of tens to hundreds of keV electrons and tens of keV protons into the inner magnetosphere occur frequently; after the injections the electrons decay slowly in the inner belt but protons in the low L region decay very fast. Intriguing similarities between lowermore » energy protons and higher-energy electrons are also found. The evolution of ring current electron and ion energy densities and energy content are examined in detail during two geomagnetic storms, one moderate and one intense. Here, the results show that the contribution of ring current electrons to the ring current energy content is much smaller than that of ring current ions (up to ~12% for the moderate storm and ~7% for the intense storm), and <35 keV electrons dominate the ring current electron energy content at the storm main phases. Though the electron energy content is usually much smaller than that of ions, the enhancement of ring current electron energy content during the moderate storm can get to ~30% of that of ring current ions, indicating a more dynamic feature of ring current electrons and important role of electrons in the ring current buildup. Lastly, the ring current electron energy density is also shown to be higher at midnight and dawn while lower at noon and dusk.« less

Surface Plasmon Enhanced Sensitive Detection for Possible Signature of Majorana Fermions via a Hybrid Semiconductor Quantum Dot-Metal Nanoparticle System

PubMed Central

Chen, Hua-Jun; Zhu, Ka-Di

2015-01-01

In the present work, we theoretically propose an optical scheme to detect the possible signature of Majorana fermions via the optical pump-probe spectroscopy, which is very different from the current tunneling measurement based on electrical methods. The scheme consists of a metal nanoparticle and a semiconductor quantum dot coupled to a hybrid semiconductor/superconductor heterostructures. The results show that the probe absorption spectrum of the quantum dot presents a distinct splitting due to the existence of Majorana fermions. Owing to surface plasmon enhanced effect, this splitting will be more obvious, which makes Majorana fermions more easy to be detectable. The technique proposed here open the door for new applications ranging from robust manipulation of Majorana fermions to quantum information processing based on Majorana fermions. PMID:26310929

Study of plasma parameters in a pulsed plasma accelerator using triple Langmuir probe

NASA Astrophysics Data System (ADS)

Borthakur, S.; Talukdar, N.; Neog, N. K.; Borthakur, T. K.

2018-01-01

A Triple Langmuir Probe (TLP) has been used to study plasma parameters of a transient plasma produced in a newly developed Pulsed Plasma Accelerator system. In this experiment, a TLP with a capacitor based current mode biasing circuit was used that instantaneously gives voltage traces in an oscilloscope which are directly proportional to the plasma electron temperature and density. The electron temperature (Te) and plasma density (ne) of the plasma are measured with the help of this probe and found to be 24.13 eV and 3.34 × 1021/m3 at the maximum energy (-15 kV) of the system, respectively. An attempt was also made to analyse the time-dependent fluctuations in plasma parameters detected by the highly sensitive triple probe. In addition to this, the variation of these parameters under different discharge voltages was studied. The information obtained from these parameters is the initial diagnostics of a new device which is to be dedicated to study the impact of high heat flux plasma stream upon material surfaces inside an ITER like tokamak.

Microencapsulated fluorescent pH probe as implantable sensor for monitoring the physiological state of fish embryos.

PubMed

Gurkov, Anton; Sadovoy, Anton; Shchapova, Ekaterina; Teh, Cathleen; Meglinski, Igor; Timofeyev, Maxim

2017-01-01

In vivo physiological measurement is a major challenge in modern science and technology, as is environment conservation at the global scale. Proper toxicological testing of widely produced mixtures of chemicals is a necessary step in the development of new products, allowing us to minimize the human impact on aquatic ecosystems. However, currently available bioassay-based techniques utilizing small aquatic organisms such as fish embryos for toxicity testing do not allow assessing in time the changes in physiological parameters in the same individual. In this study, we introduce microencapsulated fluorescent probes as a promising tool for in vivo monitoring of internal pH variation in zebrafish embryos. The pH alteration identified under stress conditions demonstrates the applicability of the microencapsulated fluorescent probes for the repeated analysis of the embryo's physiological state. The proposed approach has strong potential to simultaneously measure a range of physiological characteristics using a set of specific fluorescent probes and to finally bring toxicological bioassays and related research fields to a new level of effectiveness and sensitivity.

A Wirelessly Powered Micro-Spectrometer for Neural Probe-Pin Device

NASA Technical Reports Server (NTRS)

Choi, Sang H.; Kim, Min Hyuck; Song, Kyo D.; Yoon, Hargsoon; Lee, Uhn

2015-01-01

Treatment of neurological anomalies, places stringent demands on device functionality and size. A micro-spectrometer has been developed for use as an implantable neural probe to monitor neuro-chemistry in synapses. The microspectrometer, based on a NASA-invented miniature Fresnel grating, is capable of differentiating the emission spectra from various brain tissues. The micro-spectrometer meets the size requirements, and is able to probe the neuro-chemistry and suppression voltage typically associated with a neural anomaly. This neural probe-pin device (PPD) is equipped with wireless power technology (WPT) enabling operation in a continuous manner without requiring an implanted battery. The implanted neural PPD, together with a neural electronics interface and WPT, allow real-time measurement and control/feedback for remediation of neural anomalies. The design and performance of the combined PPD/WPT device for monitoring dopamine in a rat brain will be presented to demonstrate the current level of development. Future work on this device will involve the addition of an embedded expert system capable of performing semi-autonomous management of neural functions through a routine of sensing, processing, and control.

A wirelessly powered microspectrometer for neural probe-pin device

NASA Astrophysics Data System (ADS)

Choi, Sang H.; Kim, Min H.; Song, Kyo D.; Yoon, Hargsoon; Lee, Uhn

2015-12-01

Treatment of neurological anomalies, whether done invasively or not, places stringent demands on device functionality and size. We have developed a micro-spectrometer for use as an implantable neural probe to monitor neuro-chemistry in synapses. The micro-spectrometer, based on a NASA-invented miniature Fresnel grating, is capable of differentiating the emission spectra from various brain tissues. The micro-spectrometer meets the size requirements, and is able to probe the neuro-chemistry and suppression voltage typically associated with a neural anomaly. This neural probe-pin device (PPD) is equipped with wireless power technology (WPT) to enable operation in a continuous manner without requiring an implanted battery. The implanted neural PPD, together with a neural electronics interface and WPT, enable real-time measurement and control/feedback for remediation of neural anomalies. The design and performance of the combined PPD/WPT device for monitoring dopamine in a rat brain will be presented to demonstrate the current level of development. Future work on this device will involve the addition of an embedded expert system capable of performing semi-autonomous management of neural functions through a routine of sensing, processing, and control.

Development and Application of Chemical Probes for Vibrational Imaging by Stimulated Raman Scattering

NASA Astrophysics Data System (ADS)

Hu, Fanghao

During the last decade, Raman microscopy is experiencing rapid development and increasingly applied in biological and medical systems. Especially, stimulated Raman scattering (SRS) microscopy, which significantly improves the sensitivity of Raman scattering through stimulated emission, has allowed direct visualization of many species that are previously challenging with conventional fluorescence imaging. Compared to fluorescence, SRS imaging requires no label or small label on the target molecule, thus with minimal perturbation to the molecule of interest. Moreover, Raman scattering is free from complicated photophysical and photochemical processes such as photobleaching, and has intrinsically narrower linewidth than fluorescence emission. This allows multiplexed Raman imaging with minimal spectral crosstalk and excellent photo-stability. To achieve the full potential of Raman microscopy, vibrational probes have been developed for Raman imaging. Multiple Raman probes with a few atoms in size are applied in Raman imaging with high sensitivity and specificity. An overview of both fluorescence and Raman microscopy and their imaging probes is given in Chapter 1 with a brief discussion on the SRS theory. Built on the current progress of Raman microscopy and vibrational probes, I write on my research in the development of carbon-deuterium, alkyne and nitrile probes for visualizing choline metabolism (Chapter 2), glucose uptake activity (Chapter 3), complex brain metabolism (Chapter 4) and polymeric nanoparticles (Chapter 5) in live cells and tissues, as well as the development of polyyne-based vibrational probes for super-multiplexed imaging, barcoding and analysis (Chapter 6).

Real time Faraday spectrometer

DOEpatents

Smith, Jr., Tommy E.; Struve, Kenneth W.; Colella, Nicholas J.

1991-01-01

This invention uses a dipole magnet to bend the path of a charged particle beam. As the deflected particles exit the magnet, they are spatially dispersed in the bend-plane of the magnet according to their respective momenta and pass to a plurality of chambers having Faraday probes positioned therein. Both the current and energy distribution of the particles is then determined by the non-intersecting Faraday probes located along the chambers. The Faraday probes are magnetically isolated from each other by thin metal walls of the chambers, effectively providing real time current-versus-energy particle measurements.

Dynamic variable selection in SNP genotype autocalling from APEX microarray data.

PubMed

Podder, Mohua; Welch, William J; Zamar, Ruben H; Tebbutt, Scott J

2006-11-30

Single nucleotide polymorphisms (SNPs) are DNA sequence variations, occurring when a single nucleotide--adenine (A), thymine (T), cytosine (C) or guanine (G)--is altered. Arguably, SNPs account for more than 90% of human genetic variation. Our laboratory has developed a highly redundant SNP genotyping assay consisting of multiple probes with signals from multiple channels for a single SNP, based on arrayed primer extension (APEX). This mini-sequencing method is a powerful combination of a highly parallel microarray with distinctive Sanger-based dideoxy terminator sequencing chemistry. Using this microarray platform, our current genotype calling system (known as SNP Chart) is capable of calling single SNP genotypes by manual inspection of the APEX data, which is time-consuming and exposed to user subjectivity bias. Using a set of 32 Coriell DNA samples plus three negative PCR controls as a training data set, we have developed a fully-automated genotyping algorithm based on simple linear discriminant analysis (LDA) using dynamic variable selection. The algorithm combines separate analyses based on the multiple probe sets to give a final posterior probability for each candidate genotype. We have tested our algorithm on a completely independent data set of 270 DNA samples, with validated genotypes, from patients admitted to the intensive care unit (ICU) of St. Paul's Hospital (plus one negative PCR control sample). Our method achieves a concordance rate of 98.9% with a 99.6% call rate for a set of 96 SNPs. By adjusting the threshold value for the final posterior probability of the called genotype, the call rate reduces to 94.9% with a higher concordance rate of 99.6%. We also reversed the two independent data sets in their training and testing roles, achieving a concordance rate up to 99.8%. The strength of this APEX chemistry-based platform is its unique redundancy having multiple probes for a single SNP. Our model-based genotype calling algorithm captures the redundancy in the system considering all the underlying probe features of a particular SNP, automatically down-weighting any 'bad data' corresponding to image artifacts on the microarray slide or failure of a specific chemistry. In this regard, our method is able to automatically select the probes which work well and reduce the effect of other so-called bad performing probes in a sample-specific manner, for any number of SNPs.

Modeling of current characteristics of segmented Langmuir probe on DEMETER

DOE Office of Scientific and Technical Information (OSTI.GOV)

Imtiaz, Nadia; Marchand, Richard; Lebreton, Jean-Pierre

We model the current characteristics of the DEMETER Segmented Langmuir probe (SLP). The probe is used to measure electron density and temperature in the ionosphere at an altitude of approximately 700 km. It is also used to measure the plasma flow velocity in the satellite frame of reference. The probe is partitioned into seven collectors: six electrically insulated spherical segments and a guard electrode (the rest of the sphere and the small post). Comparisons are made between the predictions of the model and DEMETER measurements for actual ionospheric plasma conditions encountered along the satellite orbit. Segment characteristics are computed numericallymore » with PTetra, a three-dimensional particle in cell simulation code. In PTetra, space is discretized with an unstructured tetrahedral mesh, thus, enabling a good representation of the probe geometry. The model also accounts for several physical effects of importance in the interaction of spacecraft with the space environment. These include satellite charging, photoelectron, and secondary electron emissions. The model is electrostatic, but it accounts for the presence of a uniform background magnetic field. PTetra simulation results show different characteristics for the different probe segments. The current collected by each segment depends on its orientation with respect to the ram direction, the plasma composition, the magnitude, and the orientation of the magnetic field. It is observed that the presence of light H{sup +} ions leads to a significant increase in the ion current branch of the I-V curves of the negatively polarized SLP. The effect of the magnetic field is demonstrated by varying its magnitude and direction with respect to the reference magnetic field. It is found that the magnetic field appreciably affects the electron current branch of the I-V curves of certain segments on the SLP, whereas the ion current branch remains almost unaffected. PTetra simulations are validated by comparing the computed characteristics and their angular anisotropy with the DEMETER measurements, as simulation results are found to be in good agreement with the measurements.« less

The promise and peril of chemical probes

PubMed Central

Arrowsmith, Cheryl H; Audia, James E; Austin, Christopher; Baell, Jonathan; Bennett, Jonathan; Blagg, Julian; Bountra, Chas; Brennan, Paul E; Brown, Peter J; Bunnage, Mark E; Buser-Doepner, Carolyn; Campbell, Robert M; Carter, Adrian J; Cohen, Philip; Copeland, Robert A; Cravatt, Ben; Dahlin, Jayme L; Dhanak, Dashyant; Frederiksen, Mathias; Frye, Stephen V; Gray, Nathanael; Grimshaw, Charles E; Hepworth, David; Howe, Trevor; Huber, Kilian V M; Jin, Jian; Knapp, Stefan; Kotz, Joanne D; Kruger, Ryan G; Lowe, Derek; Mader, Mary M; Marsden, Brian; Mueller-Fahrnow, Anke; Müller, Susanne; O'Hagan, Ronan C; Overington, John P; Owen, Dafydd R; Rosenberg, Saul H; Ross, Ruth; Roth, Bryan; Schapira, Matthieu; Schreiber, Stuart L; Shoichet, Brian; Sundström, Michael; Superti-Furga, Giulio; Taunton, Jack; Toledo-Sherman, Leticia; Walpole, Chris; Walters, Michael A; Willson, Timothy M; Workman, Paul; Young, Robert N; Zuercher, William J

2016-01-01

Chemical probes are powerful reagents with increasing impacts on biomedical research. However, probes of poor quality or that are used incorrectly generate misleading results. To help address these shortcomings, we will create a community-driven wiki resource to improve quality and convey current best practice. PMID:26196764

Homogeneous versus heterogeneous probes for microbial ecological microarrays.

PubMed

Bae, Jin-Woo; Park, Yong-Ha

2006-07-01

Microbial ecological microarrays have been developed for investigating the composition and functions of microorganism communities in environmental niches. These arrays include microbial identification microarrays, which use oligonucleotides, gene fragments or microbial genomes as probes. In this article, the advantages and disadvantages of each type of probe are reviewed. Oligonucleotide probes are currently useful for probing uncultivated bacteria that are not amenable to gene fragment probing, whereas the functional gene fragments amplified randomly from microbial genomes require phylogenetic and hierarchical categorization before use as microbial identification probes, despite their high resolution for both specificity and sensitivity. Until more bacteria are sequenced and gene fragment probes are thoroughly validated, heterogeneous bacterial genome probes will provide a simple, sensitive and quantitative tool for exploring the ecosystem structure.

Electric field measurement in microwave discharge ion thruster with electro-optic probe.

PubMed

Ise, Toshiyuki; Tsukizaki, Ryudo; Togo, Hiroyoshi; Koizumi, Hiroyuki; Kuninaka, Hitoshi

2012-12-01

In order to understand the internal phenomena in a microwave discharge ion thruster, it is important to measure the distribution of the microwave electric field inside the discharge chamber, which is directly related to the plasma production. In this study, we proposed a novel method of measuring a microwave electric field with an electro-optic (EO) probe based on the Pockels effect. The probe, including a cooling system, contains no metal and can be accessed in the discharge chamber with less disruption to the microwave distribution. This method enables measurement of the electric field profile under ion beam acceleration. We first verified the measurement with the EO probe by a comparison with a finite-difference time domain numerical simulation of the microwave electric field in atmosphere. Second, we showed that the deviations of the reflected microwave power and the beam current were less than 8% due to inserting the EO probe into the ion thruster under ion beam acceleration. Finally, we successfully demonstrated the measurement of the electric-field profile in the ion thruster under ion beam acceleration. These measurements show that the electric field distribution in the thruster dramatically changes in the ion thruster under ion beam acceleration as the propellant mass flow rate increases. These results indicate that this new method using an EO probe can provide a useful guide for improving the propulsion of microwave discharge ion thrusters.

Automated pinhole-aperture diagnostic for the current profiling of TWT electron beams

NASA Astrophysics Data System (ADS)

Wei, Yu-Xiang; Huang, Ming-Guang; Liu, Shu-Qing; Liu, Jin-Yue; Hao, Bao-Liang; Du, Chao-Hai; Liu, Pu-Kun

2013-02-01

The measurement system reported here is intended for use in determining the current density distribution of electron beams from Pierce guns for use in TWTs. The system was designed to automatically scan the cross section of the electron beam and collect the high-resolution data with a Faraday cup probe mounted on a multistage manipulator using the LabVIEW program. A 0.06 mm thick molybdenum plate with a pinhole and a Faraday cup mounted as a probe assembly was employed to sample the electron beam current with 0.5 µm space resolution. The thermal analysis of the probe with pulse beam heating was discussed. A 0.45 µP electron gun with the expected minimum beam radius 0.42 mm was measured and the three-dimensional current density distribution, beam envelope and phase space were presented.

Contingency-based emotional resilience: effort-based reward training and flexible coping lead to adaptive responses to uncertainty in male rats

PubMed Central

Lambert, Kelly G.; Hyer, Molly M.; Rzucidlo, Amanda A.; Bergeron, Timothy; Landis, Timothy; Bardi, Massimo

2014-01-01

Emotional resilience enhances an animal's ability to maintain physiological allostasis and adaptive responses in the midst of challenges ranging from cognitive uncertainty to chronic stress. In the current study, neurobiological factors related to strategic responses to uncertainty produced by prediction errors were investigated by initially profiling male rats as passive, active or flexible copers (n = 12 each group) and assigning to either a contingency-trained or non-contingency trained group. Animals were subsequently trained in a spatial learning task so that problem solving strategies in the final probe task, as well-various biomarkers of brain activation and plasticity in brain areas associated with cognition and emotional regulation, could be assessed. Additionally, fecal samples were collected to further determine markers of stress responsivity and emotional resilience. Results indicated that contingency-trained rats exhibited more adaptive responses in the probe trial (e.g., fewer interrupted grooming sequences and more targeted search strategies) than the noncontingent-trained rats; additionally, increased DHEA/CORT ratios were observed in the contingent-trained animals. Diminished activation of the habenula (i.e., fos-immunoreactivity) was correlated with resilience factors such as increased levels of DHEA metabolites during cognitive training. Of the three coping profiles, flexible copers exhibited enhanced neuroplasticity (i.e., increased dentate gyrus doublecortin-immunoreactivity) compared to the more consistently responding active and passive copers. Thus, in the current study, contingency training via effort-based reward (EBR) training, enhanced by a flexible coping style, provided neurobiological resilience and adaptive responses to prediction errors in the final probe trial. These findings have implications for psychiatric illnesses that are influenced by altered stress responses and decision-making abilities (e.g., depression). PMID:24808837

Contingency-based emotional resilience: effort-based reward training and flexible coping lead to adaptive responses to uncertainty in male rats.

PubMed

Lambert, Kelly G; Hyer, Molly M; Rzucidlo, Amanda A; Bergeron, Timothy; Landis, Timothy; Bardi, Massimo

2014-01-01

Emotional resilience enhances an animal's ability to maintain physiological allostasis and adaptive responses in the midst of challenges ranging from cognitive uncertainty to chronic stress. In the current study, neurobiological factors related to strategic responses to uncertainty produced by prediction errors were investigated by initially profiling male rats as passive, active or flexible copers (n = 12 each group) and assigning to either a contingency-trained or non-contingency trained group. Animals were subsequently trained in a spatial learning task so that problem solving strategies in the final probe task, as well-various biomarkers of brain activation and plasticity in brain areas associated with cognition and emotional regulation, could be assessed. Additionally, fecal samples were collected to further determine markers of stress responsivity and emotional resilience. Results indicated that contingency-trained rats exhibited more adaptive responses in the probe trial (e.g., fewer interrupted grooming sequences and more targeted search strategies) than the noncontingent-trained rats; additionally, increased DHEA/CORT ratios were observed in the contingent-trained animals. Diminished activation of the habenula (i.e., fos-immunoreactivity) was correlated with resilience factors such as increased levels of DHEA metabolites during cognitive training. Of the three coping profiles, flexible copers exhibited enhanced neuroplasticity (i.e., increased dentate gyrus doublecortin-immunoreactivity) compared to the more consistently responding active and passive copers. Thus, in the current study, contingency training via effort-based reward (EBR) training, enhanced by a flexible coping style, provided neurobiological resilience and adaptive responses to prediction errors in the final probe trial. These findings have implications for psychiatric illnesses that are influenced by altered stress responses and decision-making abilities (e.g., depression).

Ion sheath dynamics in a plasma for plasma-based ion implantation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yatsuzuka, M.; Miki, S.; Azuma, K.

1999-07-01

Spatial and temporal growth and collapse of ion sheath around an electrode of a negative high-voltage pulse (voltage: {minus}10 kV, pulse duration: 10 {micro}s) have been studied in a plasma for plasma-based ion implantation. A spherical electrode of 1.9 cm in a diameter is immersed in a nitrogen plasma with the plasma density range of 10{sup 9} to 10{sup 10} cm{sup {minus}3}, the electron temperature of 1.4 eV and the gas pressure of 8x10{sup {minus}4} Torr. The transient sheath dynamics was observed by the measurement of electron saturation current to a Langmuir probe, where a depletion of electron saturation currentmore » indicates the arrival time of sheath edge at the probe position. The expanding speed of sheath edge is higher than the ion acoustic speed until the sheath length reaches the steady-state extent determined by Child-Langmuir law. In the region beyond the steady-state extent, the rarefying disturbance produced by sheath expansion continues to propagate into the plasma at the ion acoustic peed. After the pulse voltage is returned to zero (more exactly, the floating potential), the electron current begins to recover. When the pulse fall time is shorter than the plasma transit time, the electron saturation current overshoots the steady-state saturation current at once, resulting in an excess of plasma density which propagates like a tidal wave into the plasma at the ion acoustic speed.« less

An Intelligent Pinger Network for Solid Glacier Environments

NASA Astrophysics Data System (ADS)

Schönitz, S.; Reuter, S.; Henke, C.; Jeschke, S.; Ewert, D.; Eliseev, D.; Heinen, D.; Linder, P.; Scholz, F.; Weinstock, L.; Wickmann, S.; Wiebusch, C.; Zierke, S.

2016-12-01

This talk presents a novel approach for an intelligent, agent-based pinger network in an extraterrestrial glacier environment. Because of recent findings of the Cassini spacecraft, a mission to Saturn's moon Enceladus is planned in order search for extraterrestrial life within the ocean beneath Enceladus' ice crust. Therefore, a maneuverable melting probe, the EnEx probe, was developed to melt into Enceladus' ice and take liquid samples from water-filled crevasses. Hence, the probe collecting the samples has to be able to navigate in ice which is a hard problem, because neither visual nor gravitational methods can be used. To enhance the navigability of the probe, a network of autonomous pinger units (APU) is in development that is able to extract a map of the ice environment via ultrasonic soundwaves. A network of these APUs will be deployed on the surface of Enceladus, melt into the ice and form a network to help guide the probe safely to its destination. The APU network is able to form itself fully autonomously and to compensate system failures of individual APUs. The agents controlling the single APU are realized by rule-based expert systems implemented in CLIPS. The rule-based expert system evaluates available information of the environment, decides for actions to take to achieve the desired goal (e.g. a specific network topology), and executes and monitors such actions. In general, it encodes certain situations that are evaluated whenever an APU is currently idle, and then decides for a next action to take. It bases this decision on its internal world model that is shared with the other APUs. The optimal network topology that defines each agents position is iteratively determined by mixed-integer nonlinear programming. Extensive simulations studies show that the proposed agent design enables the APUs to form a robust network topology that is suited to create a reliable 3D map of the ice environment.

Numerical modeling of probe velocity effects for electromagnetic NDE methods

NASA Astrophysics Data System (ADS)

Shin, Y. K.; Lord, W.

The present discussion of magnetic flux (MLF) leakage inspection introduces the behavior of motion-induced currents. The results obtained indicate that velocity effects exist at even low probe speeds for magnetic materials, compelling the inclusion of velocity effects in MLF testing of oil pipelines, where the excitation level and pig speed are much higher than those used in the present work. Probe velocity effect studies should influence probe design, defining suitable probe speed limits and establishing training guidelines for defect-characterization schemes.

Assembling a prototype resonance electrical impedance spectroscopy system for breast tissue signal detection: preliminary assessment

NASA Astrophysics Data System (ADS)

Sumkin, Jules; Zheng, Bin; Gruss, Michelle; Drescher, John; Leader, Joseph; Good, Walter; Lu, Amy; Cohen, Cathy; Shah, Ratan; Zuley, Margarita; Gur, David

2008-03-01

Using electrical impedance spectroscopy (EIS) technology to detect breast abnormalities in general and cancer in particular has been attracting research interests for decades. Large clinical tests suggest that current EIS systems can achieve high specificity (>= 90%) at a relatively low sensitivity ranging from 15% to 35%. In this study, we explore a new resonance frequency based electrical impedance spectroscopy (REIS) technology to measure breast tissue EIS signals in vivo, which aims to be more sensitive to small tissue changes. Through collaboration between our imaging research group and a commercial company, a unique prototype REIS system has been assembled and preliminary signal acquisition has commenced. This REIS system has two detection probes mounted in the two ends of a Y-shape support device with probe separation of 60 mm. During REIS measurement, one probe touches the nipple and the other touches to an outer point of the breast. The electronic system continuously generates sweeps of multi-frequency electrical pulses ranging from 100 to 4100 kHz. The maximum electric voltage and the current applied to the probes are 1.5V and 30mA, respectively. Once a "record" command is entered, multi-frequency sweeps are recorded every 12 seconds until the program receives a "stop recording" command. In our imaging center, we have collected REIS measurements from 150 women under an IRB approved protocol. The database includes 58 biopsy cases, 78 screening negative cases, and other "recalled" cases (for additional imaging procedures). We measured eight signal features from the effective REIS sweep of each breast. We applied a multi-feature based artificial neural network (ANN) to classify between "biopsy" and normal "non-biopsy" breasts. The ANN performance is evaluated using a leave-one-out validation method and ROC analysis. We conducted two experiments. The first experiment attempted to classify 58 "biopsy" breasts and 58 "non-biopsy" breasts acquired on 58 women each having one breast recommended for biopsy. The second experiment attempted to classify 58 "biopsy" breasts and 58 negative breasts from the set of screening negative cases. The areas under ROC curves are 0.679 +/- 0.033 and 0.606 +/- 0.035 for the first and the second experiment, respectively. The preliminary results demonstrate (1) even with this rudimentary system with only one paired probes there is a measurable signal of changes in breast tissue demonstrating the feasibility of applying REIS technology for identifying at least some women with highly suspicious breast abnormalities and (2) the electromagnetic asymmetry between two breasts may be more sensitive in detecting changes in the abnormal breast. To further improve the REIS system performance, we are currently designing a new REIS system with multiple electrical probes and a more sophisticated analysis scheme.

What do you measure when you measure the Hall effect?

NASA Astrophysics Data System (ADS)

Koon, D. W.; Knickerbocker, C. J.

1993-02-01

A formalism for calculating the sensitivity of Hall measurements to local inhomogeneities of the sample material or the magnetic field is developed. This Hall weighting function g(x,y) is calculated for various placements of current and voltage probes on square and circular laminar samples. Unlike the resistivity weighting function, it is nonnegative throughout the entire sample, provided all probes lie at the edge of the sample. Singularities arise in the Hall weighting function near the current and voltage probes except in the case where these probes are located at the corners of a square. Implications of the results for cross, clover, and bridge samples, and the implications of our results for metal-insulator transition and quantum Hall studies are discussed.

Modeling and visualization of carrier motion in organic films by optical second harmonic generation and Maxwell-displacement current

NASA Astrophysics Data System (ADS)

Iwamoto, Mitsumasa; Manaka, Takaaki; Taguchi, Dai

2015-09-01

The probing and modeling of carrier motions in materials as well as in electronic devices is a fundamental research subject in science and electronics. According to the Maxwell electromagnetic field theory, carriers are a source of electric field. Therefore, by probing the dielectric polarization caused by the electric field arising from moving carriers and dipoles, we can find a way to visualize the carrier motions in materials and in devices. The techniques used here are an electrical Maxwell-displacement current (MDC) measurement and a novel optical method based on the electric field induced optical second harmonic generation (EFISHG) measurement. The MDC measurement probes changes of induced charge on electrodes, while the EFISHG probes nonlinear polarization induced in organic active layers due to the coupling of electron clouds of molecules and electro-magnetic waves of an incident laser beam in the presence of a DC field caused by electrons and holes. Both measurements allow us to probe dynamical carrier motions in solids through the detection of dielectric polarization phenomena originated from dipolar motions and electron transport. In this topical review, on the basis of Maxwell’s electro-magnetism theory of 1873, which stems from Faraday’s idea, the concept for probing electron and hole transport in solids by using the EFISHG is discussed in comparison with the conventional time of flight (TOF) measurement. We then visualize carrier transit in organic devices, i.e. organic field effect transistors, organic light emitting diodes, organic solar cells, and others. We also show that visualizing an EFISHG microscopic image is a novel way for characterizing anisotropic carrier transport in organic thin films. We also discuss the concept of the detection of rotational dipolar motions in monolayers by means of the MDC measurement, which is capable of probing the change of dielectric spontaneous polarization formed by dipoles in organic monolayers. Finally we conclude that the ideas and experiments on EFISHG and MDC lead to a novel way of analyzing dynamical motions of electrons, holes, and dipoles in solids, and thus are available in organic electronic device application.

Probe-Substrate Distance Control in Desorption Electrospray Ionization

NASA Astrophysics Data System (ADS)

Yarger, Tyler J.; Yuill, Elizabeth M.; Baker, Lane A.

2018-03-01

We introduce probe-substrate distance (Dps)-control to desorption electrospray ionization (DESI) and report a systematic investigation of key experimental parameters. Examination of voltage, flow rate, and nebulizing gas pressure suggests as Dps decreases, the distance-dependent spray current increases, until a critical point. At the critical point the relationship inverts, and the spray current decreases as the probe moves closer to the surface due to constriction of solution flow by the nebulizing gas. Dps control was used to explore the use of spray current as a signal for feedback positioning, while mass spectrometry imaging was performed simultaneously. Further development of this technique is expected to find application in study of structure-function relationships for clinical diagnostics, biological investigation, and materials characterization. [Figure not available: see fulltext.

A host-guest-recognition-based electrochemical aptasensor for thrombin detection.

PubMed

Fan, Hao; Li, Hui; Wang, Qingjiang; He, Pingang; Fang, Yuzhi

2012-05-15

A sensitive electrochemical aptasensor for thrombin detection is presented based on the host-guest recognition technique. In this sensing protocol, a 15 based thrombin aptamer (ab. TBA) was dually labeled with a thiol at its 3' end and a 4-((4-(dimethylamino)phenyl)azo) benzoic acid (dabcyl) at its 5' end, respectively, which was previously immobilized on one Au electrode surface by AuS bond and used as the thrombin probe during the protein sensing procedure. One special electrochemical marker was prepared by modifying CdS nanoparticle with β-cyclodextrins (ab. CdS-CDs), which employed as electrochemical signal provider and would conjunct with the thrombin probe modified electrode through the host-guest recognition of CDs to dabcyl. In the absence of thrombin, the probe adopted linear structure to conjunct with CdS-CDs. In present of thrombin, the TBA bond with thrombin and transformed into its special G-quarter structure, which forced CdS-CDs into the solution. Therefore, the target-TBA binding event can be sensitively transduced via detecting the electrochemical oxidation current signal of Cd of CdS nanoparticles in the solution. Using this method, as low as 4.6 pM thrombin had been detected. Copyright © 2012 Elsevier B.V. All rights reserved.

Real-time PCR based on SYBR-Green I fluorescence: an alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions.

PubMed

Ponchel, Frederique; Toomes, Carmel; Bransfield, Kieran; Leong, Fong T; Douglas, Susan H; Field, Sarah L; Bell, Sandra M; Combaret, Valerie; Puisieux, Alain; Mighell, Alan J; Robinson, Philip A; Inglehearn, Chris F; Isaacs, John D; Markham, Alex F

2003-10-13

Real-time PCR is increasingly being adopted for RNA quantification and genetic analysis. At present the most popular real-time PCR assay is based on the hybridisation of a dual-labelled probe to the PCR product, and the development of a signal by loss of fluorescence quenching as PCR degrades the probe. Though this so-called 'TaqMan' approach has proved easy to optimise in practice, the dual-labelled probes are relatively expensive. We have designed a new assay based on SYBR-Green I binding that is quick, reliable, easily optimised and compares well with the published assay. Here we demonstrate its general applicability by measuring copy number in three different genetic contexts; the quantification of a gene rearrangement (T-cell receptor excision circles (TREC) in peripheral blood mononuclear cells); the detection and quantification of GLI, MYC-C and MYC-N gene amplification in cell lines and cancer biopsies; and detection of deletions in the OPA1 gene in dominant optic atrophy. Our assay has important clinical applications, providing accurate diagnostic results in less time, from less biopsy material and at less cost than assays currently employed such as FISH or Southern blotting.

[Application of DNA-based electrochemical biosensor in rapid detection of Escherichia coli exist in licorice decoction].

PubMed

Zhao, Yu-Wen; Wang, Hai-Xia; Bie, Song-Tao; Shao, Qian; Wang, Chun-Hua; Wang, Dong-Heng; Li, Zheng

2018-03-01

A new method for detection of Escherichia coli exist in licorice decoction was developed by using DNA-based electrochemical biosensor. The thiolated capture probe was immobilized on a gold electrode at first. Then the aptamer for Escherichia coli was combined with the capture probe by hybridization. Due to the stronger interaction between the aptamer and the E. coli, the aptamer can dissociate from the capture probe in the presence of E. coli in licorice decoction. The biotinylated detection probe was hybridized with the single-strand capture probe. As a result, the electrochemical response to Escherichia coli can be measured by using differential pulse voltammetric in the presence of α-naphthyl phosphate. The plot of peak current vs. the logarithm of concentration in the range from 2.7×10² to 2.7×10⁸ CFU·mL⁻¹ displayed a linear relationship with a detection limit of 50 CFU·mL⁻¹. The relative standard deviation of 3 successive scans was 2.5%，2.1%，4.6% for 2×10²，2×10⁴，2×106：⁶ CFU·mL⁻¹ E. coli, respectively. The proposed procedure showed better specificity to E. coli in comparison to Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis. In the detection of the real extractum glycyrrhizae, the results between the proposed strategy and the GB assay showed high degree of agreement, demonstrating the designed biosensor could be utilized as a powerful tool for microbial examination for traditional Chinese medicine. Copyright© by the Chinese Pharmaceutical Association.

A NASBA on microgel-tethered molecular-beacon microarray for real-time microbial molecular diagnostics.

PubMed

Ma, Y; Dai, X; Hong, T; Munk, G B; Libera, M

2016-12-19

Despite their many advantages and successes, molecular beacon (MB) hybridization probes have not been extensively used in microarray formats because of the complicating probe-substrate interactions that increase the background intensity. We have previously shown that tethering to surface-patterned microgels is an effective means for localizing MB probes to specific surface locations in a microarray format while simultaneously maintaining them in as water-like an environment as possible and minimizing probe-surface interactions. Here we extend this approach to include both real-time detection together with integrated NASBA amplification. We fabricate small (∼250 μm × 250 μm) simplex, duplex, and five-plex assays with microarray spots of controllable size (∼20 μm diameter), position, and shape to detect bacteria and fungi in a bloodstream-infection model. The targets, primers, and microgel-tethered probes can be combined in a single isothermal reaction chamber with no post-amplification labelling. We extract total RNA from clinical blood samples and differentiate between Gram-positive and Gram-negative bloodstream infection in a duplex assay to detect RNA- amplicons. The sensitivity based on our current protocols in a simplex assay to detect specific ribosomal RNA sequences within total RNA extracted from S. aureus and E. coli cultures corresponds to tens of bacteria per ml. We furthermore show that the platform can detect RNA- amplicons from synthetic target DNA with 1 fM sensitivity in sample volumes that contain about 12 000 DNA molecules. These experiments demonstrate an alternative approach that can enable rapid and real-time microarray-based molecular diagnostics.

The radio-frequency fluctuation effect on the floating harmonic method

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lee, Jaewon; Kim, Kyung-Hyun; Kim, Dong-Hwan

2016-08-15

The radio-frequency (RF) plasma diagnostics with an electrical probe facing a challenge, because the RF fluctuation oscillates the plasma potential and distorts the current-voltage (I-V) curve. As Langmuir probe is widely used in plasma diagnostics, many researchers have been studying the effect of RF fluctuation on probe and compensation methods. On the other hand, there have not been enough studies on the fluctuation effect on the floating harmonic method. Therefore, we investigated the impact of RF fluctuation on the floating harmonic method theoretically and experimentally. When the electrons are in ideal Maxwellian distribution, the floating potential is negatively shifted bymore » the RF fluctuation, but the fluctuation does not distort I-V curve around the floating potential. However, in practical plasmas, the I-V curve and their harmonic components are distorted. This RF fluctuation effect becomes more significant in a low density plasma with a high impedance sheath. The second harmonic current decreases with the RF fluctuation while the first harmonic current is merely affected. Therefore, the electron temperatures measured with the floating harmonic method under low density plasma with uncompensated probe are overestimated than the results obtained with the compensated probe.« less

Magnetic susceptibility well-logging unit with single power supply thermoregulation system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Seeley, R. L.

1985-11-05

The magnetic susceptibility well-logging unit with single power supply thermoregulation system provides power from a single surface power supply over a well-logging cable to an integrated circuit voltage regulator system downhole. This voltage regulator system supplies regulated voltages to a temperature control system and also to a Maxwell bridge sensing unit which includes the solenoid of a magnetic susceptibility probe. The temperature control system is provided with power from the voltage regulator system and operates to permit one of several predetermined temperatures to be chosen, and then operates to maintain the solenoid of a magnetic susceptibility probe at this chosenmore » temperature. The temperature control system responds to a temperature sensor mounted upon the probe solenoid to cause resistance heaters concentrically spaced from the probe solenoid to maintain the chosen temperature. A second temperature sensor on the probe solenoid provides a temperature signal to a temperature transmitting unit, which initially converts the sensed temperature to a representative voltage. This voltage is then converted to a representative current signal which is transmitted by current telemetry over the well logging cable to a surface electronic unit which then reconverts the current signal to a voltage signal.« less

Investigation of eddy current examination on OD fatigue crack for steam generator tubes

NASA Astrophysics Data System (ADS)

Kong, Yuying; Ding, Boyuan; Li, Ming; Liu, Jinhong; Chen, Huaidong; Meyendorf, Norbert G.

2015-03-01

The opening width of fatigue crack was very small, and conventional Bobbin probe was very difficult to detect it in steam generator tubes. Different sizes of 8 fatigue cracks were inspected using bobbin probe rotating probe. The analysis results showed that, bobbin probe was not sensitive for fatigue crack even for small through wall crack mixed with denting signal. On the other hand, the rotating probe was easily to detect all cracks. Finally, the OD phase to depth curve for fatigue crack using rotating probe was established and the results agreed very well with the true crack size.

The use of segmented cathodes to determine the spoke current density distribution in high power impulse magnetron sputtering plasmas

DOE Office of Scientific and Technical Information (OSTI.GOV)

Poolcharuansin, Phitsanu; The Technological Plasma Research Unit, Department of Physics, Mahasarakham University, Maha Sarakham 44150; Estrin, Francis Lockwood

2015-04-28

The localized target current density associated with quasi-periodic ionization zones (spokes) has been measured in a high power impulse magnetron sputtering (HiPIMS) discharge using an array of azimuthally separated and electrical isolated probes incorporated into a circular aluminum target. For a particular range of operating conditions (pulse energies up to 2.2 J and argon pressures from 0.2 to 1.9 Pa), strong oscillations in the probe current density are seen with amplitudes up to 52% above a base value. These perturbations, identified as spokes, travel around the discharge above the target in the E×B direction. Using phase information from the angularly separated probes,more » the spoke drift speeds, angular frequencies, and mode number have been determined. Generally, at low HiPIMS pulse energies E{sub p} < 0.8 J, spokes appear to be chaotic in nature (with random arrival times), however as E{sub p} increases, coherent spokes are observed with velocities between 6.5 and 10 km s{sup −1} and mode numbers m = 3 or above. At E{sub p} > 1.8 J, the plasma becomes spoke-free. The boundaries between chaotic, coherent, and no-spoke regions are weakly dependent on pressure. During each HiPIMS pulse, the spoke velocities increase by about 50%. Such an observation is explained by considering spoke velocities to be determined by the critical ionization velocity, which changes as the plasma composition changes during the pulse. From the shape of individual current density oscillations, it appears that the leading edge of the spoke is associated with a slow increase in local current density to the target and the rear with a more rapid decrease. The measurements show that the discharge current density associated with individual spokes is broadly spread over a wide region of the target.« less

Active versus passive maintenance of visual nonverbal memory.

PubMed

McKeown, Denis; Holt, Jessica; Delvenne, Jean-Francois; Smith, Amy; Griffiths, Benjamin

2014-08-01

Forgetting over the short term has challenged researchers for more than a century, largely because of the difficulty of controlling what goes on within the memory retention interval. But the "recent-negative-probe" procedure offers a valuable paradigm, by examining the influences of (presumably) unattended memoranda from prior trials. Here we used a recent-probe task to investigate forgetting for visual nonverbal short-term memory. The target stimuli (two visually presented abstract shapes) on a trial were followed after a retention interval by a probe, and participants indicated whether the probe matched one of the target items. Proactive interference, and hence memory for old trial probes, was observed, whereby participants were slowed in rejecting a nonmatching probe on the current trial that nevertheless matched a target item on the previous trial (a recent-negative probe). The attraction of the paradigm is that, by uncovering proactive influences of past-trial probe stimuli, it can be argued that active maintenance in memory of those probes is unlikely. In two experiments, we recorded such proactive interference of prior-trial items over a range of interstimulus (ISI) and intertrial (ITI) intervals (between 1 and 6 s, respectively). Consistent with a proposed two-process memory conception (the active-passive memory model, or APM), actively maintained memories on current trials decayed, but passively "maintained," or unattended, visual memories of stimuli on past trials did not.

Dual-Modal Magnetic Resonance/Fluorescent Zinc Probes for Pancreatic β-Cell Mass Imaging

PubMed Central

Stasiuk, Graeme J; Minuzzi, Florencia; Sae-Heng, Myra; Rivas, Charlotte; Juretschke, Hans-Paul; Piemonti, Lorenzo; Allegrini, Peter R; Laurent, Didier; Duckworth, Andrew R; Beeby, Andrew; Rutter, Guy A; Long, Nicholas J

2015-01-01

Despite the contribution of changes in pancreatic β-cell mass to the development of all forms of diabetes mellitus, few robust approaches currently exist to monitor these changes prospectively in vivo. Although magnetic-resonance imaging (MRI) provides a potentially useful technique, targeting MRI-active probes to the β cell has proved challenging. Zinc ions are highly concentrated in the secretory granule, but they are relatively less abundant in the exocrine pancreas and in other tissues. We have therefore developed functional dual-modal probes based on transition-metal chelates capable of binding zinc. The first of these, Gd⋅1, binds ZnII directly by means of an amidoquinoline moiety (AQA), thus causing a large ratiometric Stokes shift in the fluorescence from λem=410 to 500 nm with an increase in relaxivity from r1=4.2 up to 4.9 mM−1 s−1. The probe is efficiently accumulated into secretory granules in β-cell-derived lines and isolated islets, but more poorly by non-endocrine cells, and leads to a reduction in T1 in human islets. In vivo murine studies of Gd⋅1 have shown accumulation of the probe in the pancreas with increased signal intensity over 140 minutes. PMID:25736590

Development of a 16S rRNA-targeted fluorescence in situ hybridization probe for quantification of the ammonia-oxidizer Nitrosotalea devanaterra and its relatives.

PubMed

Restrepo-Ortiz, C X; Merbt, S N; Barrero-Canossa, J; Fuchs, B M; Casamayor, E O

2018-04-28

The Thaumarchaeota SAGMCG-1 group and, in particular, members of the genus Nitrosotalea have high occurrence in acidic soils, the rhizosphere, groundwater and oligotrophic lakes, and play a potential role in nitrogen cycling. In this study, the specific oligonucleotide fluorescence in situ hybridization probe SAG357 was designed for this Thaumarchaeota group based on the available 16S rRNA gene sequences in databases, and included the ammonia-oxidizing species Nitrosotalea devanaterra. Cell permeabilization for catalyzed reporter deposition fluorescence in situ detection and the hybridization conditions were optimized on enrichment cultures of the target species N. devanaterra, as well as the non-target ammonia-oxidizing archaeon Nitrosopumilus maritimus. Probe specificity was improved with a competitor oligonucleotide, and fluorescence intensity and cell visualization were enhanced by the design and application of two adjacent helpers. Probe performance was tested in soil samples along a pH gradient, and counting results matched the expected in situ distributions. Probe SAG357 and the CARD-FISH protocol developed in the present study will help to improve the current understanding of the ecology and physiology of N. devanaterra and its relatives in natural environments. Copyright © 2018 Elsevier GmbH. All rights reserved.

A Gradient-Field Pulsed Eddy Current Probe for Evaluation of Hidden Material Degradation in Conductive Structures Based on Lift-Off Invariance

PubMed Central

Li, Yong; Jing, Haoqing; Zainal Abidin, Ilham Mukriz; Yan, Bei

2017-01-01

Coated conductive structures are widely adopted in such engineering fields as aerospace, nuclear energy, etc. The hostile and corrosive environment leaves in-service coated conductive structures vulnerable to Hidden Material Degradation (HMD) occurring under the protection coating. It is highly demanded that HMD can be non-intrusively assessed using non-destructive evaluation techniques. In light of the advantages of Gradient-field Pulsed Eddy Current technique (GPEC) over other non-destructive evaluation methods in corrosion evaluation, in this paper the GPEC probe for quantitative evaluation of HMD is intensively investigated. Closed-form expressions of GPEC responses to HMD are formulated via analytical modeling. The Lift-off Invariance (LOI) in GPEC signals, which makes the HMD evaluation immune to the variation in thickness of the protection coating, is introduced and analyzed through simulations involving HMD with variable depths and conductivities. A fast inverse method employing magnitude and time of the LOI point in GPEC signals for simultaneously evaluating the conductivity and thickness of HMD region is proposed, and subsequently verified by finite element modeling and experiments. It has been found from the results that along with the proposed inverse method the GPEC probe is applicable to evaluation of HMD in coated conductive structures without much loss in accuracy. PMID:28441328

A Gradient-Field Pulsed Eddy Current Probe for Evaluation of Hidden Material Degradation in Conductive Structures Based on Lift-Off Invariance.

PubMed

Li, Yong; Jing, Haoqing; Zainal Abidin, Ilham Mukriz; Yan, Bei

2017-04-25

Coated conductive structures are widely adopted in such engineering fields as aerospace, nuclear energy, etc. The hostile and corrosive environment leaves in-service coated conductive structures vulnerable to Hidden Material Degradation (HMD) occurring under the protection coating. It is highly demanded that HMD can be non-intrusively assessed using non-destructive evaluation techniques. In light of the advantages of Gradient-field Pulsed Eddy Current technique (GPEC) over other non-destructive evaluation methods in corrosion evaluation, in this paper the GPEC probe for quantitative evaluation of HMD is intensively investigated. Closed-form expressions of GPEC responses to HMD are formulated via analytical modeling. The Lift-off Invariance (LOI) in GPEC signals, which makes the HMD evaluation immune to the variation in thickness of the protection coating, is introduced and analyzed through simulations involving HMD with variable depths and conductivities. A fast inverse method employing magnitude and time of the LOI point in GPEC signals for simultaneously evaluating the conductivity and thickness of HMD region is proposed, and subsequently verified by finite element modeling and experiments. It has been found from the results that along with the proposed inverse method the GPEC probe is applicable to evaluation of HMD in coated conductive structures without much loss in accuracy.

Effects of the local environment on CNT-based nanoelectronics: development of a microenvironment probe station.

PubMed

McClain, Devon; Thomas, Nicole; Nguyen, Tri; O'Brien, Kevin P; Jiao, Jun

2010-11-01

In this study, we report the development of a microenvironment probe station capable of detecting the effect of small changes to the local environment around a carbon nanotube conduction channel. The microenvironment probe station is highly versatile and is used to characterize alterations in carbon nanotube field effect transistor electrical behavior in response to changes in temperature, gas species, infrared and ultraviolet light. All devices were electrically characterized in atmospheric, ultrahigh vacuum and oxygen-rich environments. The results suggest that devices could be changed from n-type at 1 x 10(-8) torr through an intermediate ambipolar state at 1 x 10(-4) torr to p-type at atmosphere solely by increasing the oxygen concentration. The average resistance of these carbon nanotube field effect transistors after annealing was observed to decrease by approximately 54% from their initial value under ultrahigh vacuum to their final value in the presence of pure oxygen while corresponding threshold voltages shifts were also observed. Illumination with infrared light resulted in a approximately 10% increase in drain current with an estimated response time <1 fs due to photon-induced electron-hole pair generation. Illumination with ultraviolet light resulted in approximately 5-15% reduction in drain current due to photon-induced desorption of oxygen adsorbate.

A pulsed eddy current probe for inspection of support plates from within Alloy-800 steam generator tubes

NASA Astrophysics Data System (ADS)

Krause, T. W.; Babbar, V. K.; Underhill, P. R.

2014-02-01

Support plate degradation and fouling in nuclear steam generators (SGs) can lead to SG tube corrosion and loss of efficiency. Inspection and monitoring of these conditions can be integrated with preventive maintenance programs, thereby advancing station-life management processes. A prototype pulsed eddy current (PEC) probe, targeting inspection issues associated with SG tubes in SS410 tube support plate structures, has been developed using commercial finite element (FE) software. FE modeling was used to identify appropriate driver and pickup coil configurations for optimum sensitivity to changes in gap and offset for Alloy-800 SG tubes passing through 25 mm thick SS410 support plates. Experimental measurements using a probe that was manufactured based on the modeled configuration, were used to confirm the sensitivity of differential PEC signals to changes in relative position of the tube within the tube support plate holes. Models investigated the effect of shift and tilt of tube with respect to hole centers. Near hole centers and for small shifts, modeled signal amplitudes from the differentially connected coil pairs were observed to change linearly with tube shift. This was in agreement with experimentally measured TEC coil response. The work paves the way for development of a system targeting the inspection and evaluation of support plate structures in steam generators.

Biasing, acquisition, and interpretation of a dense Langmuir probe array in NSTX.

PubMed

Jaworski, M A; Kallman, J; Kaita, R; Kugel, H; LeBlanc, B; Marsala, R; Ruzic, D N

2010-10-01

A dense array of 99 Langmuir probes has been installed in the lower divertor region of the National Spherical Torus Experiment (NSTX). This array is instrumented with a system of electronics that allows flexibility in the choice of probes to bias as well as the type of measurement (including standard swept, single probe, triple probe, and operation as passive floating potential and scrape-off-layer SOL current monitors). The use of flush-mounted probes requires careful interpretation. The time dependent nature of the SOL makes swept-probe traces difficult to interpret. To overcome these challenges, the single- and triple-Langmuir probe signals are used in complementary fashion to determine the temperature and density at the probe location. A comparison to midplane measurements is made.

Development of a novel gamma probe for detecting radiation direction

NASA Astrophysics Data System (ADS)

Pani, R.; Pellegrini, R.; Cinti, M. N.; Longo, M.; Donnarumma, R.; D'Alessio, A.; Borrazzo, C.; Pergola, A.; Ridolfi, S.; De Vincentis, G.

2016-01-01

Spatial localization of radioactive sources is currently a main issue interesting different fields, including nuclear industry, homeland security as well as medical imaging. It is currently achieved using different systems, but the development of technologies for detecting and characterizing radiation is becoming important especially in medical imaging. In this latter field, radiation detection probes have long been used to guide surgery, thanks to their ability to localize and quantify radiopharmaceutical uptake even deep in tissue. Radiolabelled colloid is injected into, or near to, the tumor and the surgeon uses a hand-held radiation detector, the gamma probe, to identify lymph nodes with radiopharmaceutical uptkake. The present work refers to a novel scintigraphic goniometric probe to identify gamma radiation and its direction. The probe incorporates several scintillation crystals joined together in a particular configuration to provide data related to the position of a gamma source. The main technical characteristics of the gamma locator prototype, i.e. sensitivity, spatial resolution and detection efficiency, are investigated. Moreover, the development of a specific procedure applied to the images permits to retrieve the source position with high precision with respect to the currently used gamma probes. The presented device shows a high sensitivity and efficiency to identify gamma radiation taking a short time (from 30 to 60 s). Even though it was designed for applications in radio-guided surgery, it could be used for other purposes, as for example homeland security.

Electrochemical detection of sequence-specific DNA based on formation of G-quadruplex-hemin through continuous hybridization chain reaction.

PubMed

Sun, Xiaofan; Chen, Haohan; Wang, Shuling; Zhang, Yiping; Tian, Yaping; Zhou, Nandi

2018-08-27

A high-sensitive detection of sequence-specific DNA was established based on the formation of G-quadruplex-hemin complex through continuous hybridization chain reaction (HCR). Taking HIV DNA sequence as an example, a capture probe complementary to part of HIV DNA was firstly self-assembled onto the surface of Au electrode. Then a specially designed assistant probe with both terminals complementary to the target DNA and a G-quadruplex-forming sequence in the center was introduced into the detection solution. In the presence of both the target DNA and the assistant probe, the target DNA can be captured on the electrode surface and then a continuous HCR can be conducted due to the mutual recognition of the target DNA and the assistant probe, leading to the formation of a large number of G-quadruplex on the electrode surface. With the help of hemin, a pronounced electrochemical signal can be observed in differential pulse voltammetry (DPV), due to the formation of G-quadruplex-hemin complex. The peak current is linearly related with the logarithm of the concentration of the target DNA in the range from 10 fM to 10 pM. The electrochemical sensor has high selectivity to clearly discriminate single-base mismatched and three-base mismatched sequences from the original HIV DNA sequence. Moreover, the established DNA sensor was challenged by detection of HIV DNA in human serum samples, which showed the low detection limit of 6.3 fM. Thus it has great application prospect in the field of clinical diagnosis and environmental monitoring. Copyright © 2018 Elsevier B.V. All rights reserved.

Development of a Double Hemispherical Probe for Improved Space Plasma Measurements

NASA Astrophysics Data System (ADS)

Wang, X.; Samaniego, J. I.; Hsu, H.-W.; Horányi, M.; Wahlund, J.-E.; Ergun, R. E.; Bering, E. A.

2018-04-01

Langmuir probes have been widely used for space plasma measurements for decades. However, there are still challenges in the interpretation of their measurements. Due to the interaction of the ambient plasma with a spacecraft and an onboard probe itself, the local plasma conditions around the probe could be very different from the true ambient plasma of interest. These local plasma conditions are often anisotropic and/or inhomogeneous. Most of the Langmuir probes that are made of a single electrode have difficulties to remove these local plasma effects, introducing errors in the derived plasma characteristics. Directional probes are able to characterize anisotropic and inhomogeneous plasmas. The split Langmuir probe and the Segmented Langmuir Probe have been developed to characterize the plasma flow in the Earth's ionosphere. Here we introduce a new type of a directional Langmuir probe, the Double Hemispherical Probe (DHP), to improve the space plasma measurements in a broad range of scenarios: (a) low-density plasmas, (b) high surface-emission (photo and/or secondary electron emission) environments, (c) flowing plasmas, and (d) dust-rich plasma environments. The DHP consists of two identical hemispheres that are electrically insulated and swept with the same voltages simultaneously. The difference currents between the two hemispheres are used to characterize the anisotropic/inhomogeneous plasma conditions created around the probe, which will be then removed or minimized on the interpretation of their current-voltage curves. This paper describes the basic concept and design of the DHP sensor, as well as its initial results tested in the laboratory plasma environments.

Getting a grip on glycans: A current overview of the metabolic oligosaccharide engineering toolbox.

PubMed

Sminia, Tjerk J; Zuilhof, Han; Wennekes, Tom

2016-11-29

This review discusses the advances in metabolic oligosaccharide engineering (MOE) from 2010 to 2016 with a focus on the structure, preparation, and reactivity of its chemical probes. A brief historical overview of MOE is followed by a comprehensive overview of the chemical probes currently available in the MOE molecular toolbox and the bioconjugation techniques they enable. The final part of the review focusses on the synthesis of a selection of probes and finishes with an outlook on recent and potential upcoming advances in the field of MOE. Copyright © 2016 Elsevier Ltd. All rights reserved.

Simplified Numerical Analysis of ECT Probe - Eddy Current Benchmark Problem 3

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sikora, R.; Chady, T.; Gratkowski, S.

2005-04-09

In this paper a third eddy current benchmark problem is considered. The objective of the benchmark is to determine optimal operating frequency and size of the pancake coil designated for testing tubes made of Inconel. It can be achieved by maximization of the change in impedance of the coil due to a flaw. Approximation functions of the probe (coil) characteristic were developed and used in order to reduce number of required calculations. It results in significant speed up of the optimization process. An optimal testing frequency and size of the probe were achieved as a final result of the calculation.

Ion Current Density Study of the NASA-300M and NASA-457Mv2 Hall Thrusters

NASA Technical Reports Server (NTRS)

Huang, Wensheng; Shastry, Rohit; Herman, Daniel A.; Soulas, George C.; Kamhawi, Hani

2012-01-01

NASA Glenn Research Center is developing a Hall thruster in the 15-50 kW range to support future NASA missions. As a part of the process, the performance and plume characteristics of the NASA-300M, a 20-kW Hall thruster, and the NASA-457Mv2, a 50-kW Hall thruster, were evaluated. The collected data will be used to improve the fidelity of the JPL modeling tool, Hall2De, which will then be used to aid the design of the 15-50 kW Hall thruster. This paper gives a detailed overview of the Faraday probe portion of the plume characterization study. The Faraday probe in this study is a near-field probe swept radially at many axial locations downstream of the thruster exit plane. Threshold-based integration limits with threshold values of 1/e, 1/e2, and 1/e3 times the local peak current density are tried for the purpose of ion current integration and divergence angle calculation. The NASA-300M is operated at 7 conditions and the NASA-457Mv2 at 14 conditions. These conditions span discharge voltages of 200 to 500 V and discharge power of 10 to 50 kW. The ion current density profiles of the near-field plume originating from the discharge channel are discovered to strongly resemble Gaussian distributions. A novel analysis approach involving a form of ray tracing is used to determine an effective point of origin for the near-field plume. In the process of performing this analysis, definitive evidence is discovered that showed the near-field plume is bending towards the thruster centerline.

Ion Current Density Study of the NASA-300M and NASA-457Mv2 Hall Thrusters

NASA Technical Reports Server (NTRS)

Huang, Wensheng; Shastry, Rohit; Herman, Daniel A.; Soulas, George C.; Kamhawi, Hani

2012-01-01

NASA Glenn Research Center is developing a Hall thruster in the 15-50 kW range to support future NASA missions. As a part of the process, the performance and plume characteristics of the NASA-300M, a 20-kW Hall thruster, and the NASA-457Mv2, a 50-kW Hall thruster, were evaluated. The collected data will be used to improve the fidelity of the JPL modeling tool, Hall2De, which will then be used to aid the design of the 15-50 kW Hall thruster. This paper gives a detailed overview of the Faraday probe portion of the plume characterization study. The Faraday probe in this study is a near-field probe swept radially at many axial locations downstream of the thruster exit plane. Threshold-based integration limits with threshold values of 1/e, 1/e(sup 2), and 1/e(sup 3) times the local peak current density are tried for the purpose of ion current integration and divergence angle calculation. The NASA-300M is operated at 7 conditions and the NASA-457Mv2 at 14 conditions. These conditions span discharge voltages of 200 to 500 V and discharge power of 10 to 50 kW. The ion current density profiles of the near-field plume originating from the discharge channel are discovered to strongly resemble Gaussian distributions. A novel analysis approach involving a form of ray tracing is used to determine an effective point of origin for the near-field plume. In the process of performing this analysis, definitive evidence is discovered that showed the near-field plume is bending towards the thruster centerline.

Noninvasive techniques for probing neurocircuitry and treating illness: vagus nerve stimulation (VNS), transcranial magnetic stimulation (TMS) and transcranial direct current stimulation (tDCS)

PubMed Central

George, Mark S; Aston-Jones, Gary

2010-01-01

Although the preceding chapters discuss much of the new knowledge of neurocircuitry of neuropsychiatric diseases, and an invasive approach to treatment, this chapter describes and reviews the noninvasive methods of testing circuit-based theories and treating neuropsychiatric diseases that do not involve implanting electrodes into the brain or on its surface. These techniques are transcranial magnetic stimulation, vagus nerve stimulation, and transcranial direct current stimulation. Two of these approaches have FDA approval as therapies. PMID:19693003

Electron transport in zinc-blende wurtzite biphasic gallium nitride nanowires and GaNFETs

DOE PAGES

Jacobs, Benjamin W.; Ayres, Virginia M.; Stallcup, Richard E.; ...

2007-10-19

Two-point and four-point probe electrical measurements of a biphasic gallium nitride nanowire and current–voltage characteristics of a gallium nitride nanowire based field effect transistor are reported. The biphasic gallium nitride nanowires have a crystalline homostructure consisting of wurtzite and zinc-blende phases that grow simultaneously in the longitudinal direction. There is a sharp transition of one to a few atomic layers between each phase. Here, all measurements showed high current densities. Evidence of single-phase current transport in the biphasic nanowire structure is discussed.

Solar probe shield developmental testing

NASA Technical Reports Server (NTRS)

Miyake, Robert N.

1991-01-01

The objectives of the Solar Probe mission and the current status of the Solar Probe thermal shield subsystem development are described. In particular, the discussion includes a brief description of the mission concepts, spacecraft configuration and shield concept, material selection criteria, and the required material testing to provide a database to support the development of the shield system.

Fast tomographic methods for the tokamak ISTTOK

NASA Astrophysics Data System (ADS)

Carvalho, P. J.; Thomsen, H.; Gori, S.; Toussaint, U. v.; Weller, A.; Coelho, R.; Neto, A.; Pereira, T.; Silva, C.; Fernandes, H.

2008-04-01

The achievement of long duration, alternating current discharges on the tokamak IST-TOK requires a real-time plasma position control system. The plasma position determination based on magnetic probes system has been found to be inadequate during the current inversion due to the reduced plasma current. A tomography diagnostic has been therefore installed to supply the required feedback to the control system. Several tomographic methods are available for soft X-ray or bolo-metric tomography, among which the Cormack and Neural networks methods stand out due to their inherent speed of up to 1000 reconstructions per second, with currently available technology. This paper discusses the application of these algorithms on fusion devices while comparing performance and reliability of the results. It has been found that although the Cormack based inversion proved to be faster, the neural networks reconstruction has fewer artifacts and is more accurate.

New environment-sensitive multichannel DNA fluorescent label for investigation of the protein-DNA interactions.

PubMed

Kuznetsova, Alexandra A; Kuznetsov, Nikita A; Vorobjev, Yuri N; Barthes, Nicolas P F; Michel, Benoît Y; Burger, Alain; Fedorova, Olga S

2014-01-01

Here, we report the study of a new multichannel DNA fluorescent base analogue 3-hydroxychromone (3HC) to evaluate its suitability as a fluorescent reporter probe of structural transitions during protein-DNA interactions and its comparison with the current commercially available 2-aminopurine (aPu), pyrrolocytosine (Cpy) and 1,3-diaza-2-oxophenoxazine (tCO). For this purpose, fluorescent base analogues were incorporated into DNA helix on the opposite or on the 5'-side of the damaged nucleoside 5,6-dihydrouridine (DHU), which is specifically recognized and removed by Endonuclease VIII. These fluorophores demonstrated different sensitivities to the DNA helix conformational changes. The highest sensitivity and the most detailed information about the conformational changes of DNA induced by protein binding and processing were obtained using the 3HC probe. The application of this new artificial fluorescent DNA base is a very useful tool for the studies of complex mechanisms of protein-DNA interactions. Using 3HC biosensor, the kinetic mechanism of Endonuclease VIII action was specified.

New Environment-Sensitive Multichannel DNA Fluorescent Label for Investigation of the Protein-DNA Interactions

PubMed Central

Vorobjev, Yuri N.; Barthes, Nicolas P. F.; Michel, Benoît Y.; Burger, Alain; Fedorova, Olga S.

2014-01-01

Here, we report the study of a new multichannel DNA fluorescent base analogue 3-hydroxychromone (3HC) to evaluate its suitability as a fluorescent reporter probe of structural transitions during protein-DNA interactions and its comparison with the current commercially available 2-aminopurine (aPu), pyrrolocytosine (Cpy) and 1,3-diaza-2-oxophenoxazine (tCO). For this purpose, fluorescent base analogues were incorporated into DNA helix on the opposite or on the 5′-side of the damaged nucleoside 5,6-dihydrouridine (DHU), which is specifically recognized and removed by Endonuclease VIII. These fluorophores demonstrated different sensitivities to the DNA helix conformational changes. The highest sensitivity and the most detailed information about the conformational changes of DNA induced by protein binding and processing were obtained using the 3HC probe. The application of this new artificial fluorescent DNA base is a very useful tool for the studies of complex mechanisms of protein-DNA interactions. Using 3HC biosensor, the kinetic mechanism of Endonuclease VIII action was specified. PMID:24925085

A rapid low-cost high-density DNA-based multi-detection test for routine inspection of meat species.

PubMed

Lin, Chun Chi; Fung, Lai Ling; Chan, Po Kwok; Lee, Cheuk Man; Chow, Kwok Fai; Cheng, Shuk Han

2014-02-01

The increasing occurrence of food frauds suggests that species identification should be part of food authentication. Current molecular-based species identification methods have their own limitations or drawbacks, such as relatively time-consuming experimental steps, expensive equipment and, in particular, these methods cannot identify mixed species in a single experiment. This project proposes an improved method involving PCR amplification of the COI gene and detection of species-specific sequences by hybridisation. Major innovative breakthrough lies in the detection of multiple species, including pork, beef, lamb, horse, cat, dog and mouse, from a mixed sample within a single experiment. The probes used are species-specific either in sole or mixed species samples. As little as 5 pg of DNA template in the PCR is detectable in the proposed method. By designing species-specific probes and adopting reverse dot blot hybridisation and flow-through hybridisation, a low-cost high-density DNA-based multi-detection test suitable for routine inspection of meat species was developed. © 2013.

Single-walled carbon nanotubes based chemiresistive genosensor for label-free detection of human rheumatic heart disease

NASA Astrophysics Data System (ADS)

Singh, Swati; Kumar, Ashok; Khare, Shashi; Mulchandani, Ashok; Rajesh

2014-11-01

A specific and ultrasensitive, label free single-walled carbon nanotubes (SWNTs) based chemiresistive genosensor was fabricated for the early detection of Streptococcus pyogenes infection in human causing rheumatic heart disease. The mga gene of S. pyogenes specific 24 mer ssDNA probe was covalently immobilized on SWNT through a molecular bilinker, 1-pyrenemethylamine, using carbodiimide coupling reaction. The sensor was characterized by the current-voltage (I-V) characteristic curve and scanning electron microscopy. The sensing performance of the sensor was studied with respect to changes in conductance in SWNT channel based on hybridization of the target S. pyogenes single stranded genomic DNA (ssG-DNA) to its complementary 24 mer ssDNA probe. The sensor shows negligible response to non-complementary Staphylococcus aureus ssG-DNA, confirming the specificity of the sensor only with S. pyogenes. The genosensor exhibited a linear response to S. pyogenes G-DNA from 1 to1000 ng ml-1 with a limit of detection of 0.16 ng ml-1.

ISS Plasma Interaction: Measurements and Modeling

NASA Technical Reports Server (NTRS)

Barsamian, H.; Mikatarian, R.; Alred, J.; Minow, J.; Koontz, S.

2004-01-01

Ionospheric plasma interaction effects on the International Space Station are discussed in the following paper. The large structure and high voltage arrays of the ISS represent a complex system interacting with LEO plasma. Discharge current measurements made by the Plasma Contactor Units and potential measurements made by the Floating Potential Probe delineate charging and magnetic induction effects on the ISS. Based on theoretical and physical understanding of the interaction phenomena, a model of ISS plasma interaction has been developed. The model includes magnetic induction effects, interaction of the high voltage solar arrays with ionospheric plasma, and accounts for other conductive areas on the ISS. Based on these phenomena, the Plasma Interaction Model has been developed. Limited verification of the model has been performed by comparison of Floating Potential Probe measurement data to simulations. The ISS plasma interaction model will be further tested and verified as measurements from the Floating Potential Measurement Unit become available, and construction of the ISS continues.

Negative differential resistance observation in complex convoluted fullerene junctions

NASA Astrophysics Data System (ADS)

Kaur, Milanpreet; Sawhney, Ravinder Singh; Engles, Derick

2018-04-01

In this work, we simulated the smallest fullerene molecule, C20 in a two-probe device model with gold electrodes. The gold electrodes comprised of (011) miller planes were carved to construct the novel geometry based four unique shapes, which were strung to fullerene molecules through mechanically controlled break junction techniques. The organized devices were later scrutinized using non-equilibrium Green's function based on the density functional theory to calculate their molecular orbitals, energy levels, charge transfers, and electrical parameters. After intense scrutiny, we concluded that five-edged and six-edged devices have the lowest and highest current-conductance values, which result from their electrode-dominating and electrode-subsidiary effects, respectively. However, an interesting observation was that the three-edged and four-edged electrodes functioned as semi-metallic in nature, allowing the C20 molecule to demonstrate its performance with the complementary effect of these electrodes in the electron conduction process of a two-probe device.

Magnetometry with Low-Resistance Proximity Josephson Junction

NASA Astrophysics Data System (ADS)

Jabdaraghi, R. N.; Peltonen, J. T.; Golubev, D. S.; Pekola, J. P.

2018-06-01

We characterize a niobium-based superconducting quantum interference proximity transistor (Nb-SQUIPT) and its key constituent formed by a Nb-Cu-Nb SNS weak link. The Nb-SQUIPT and SNS devices are fabricated simultaneously in two separate lithography and deposition steps, relying on Ar ion cleaning of the Nb contact surfaces. The quality of the Nb-Cu interface is characterized by measuring the temperature-dependent equilibrium critical supercurrent of the SNS junction. In the Nb-SQUIPT device, we observe a maximum flux-to-current transfer function value of about 55 nA/Φ_0 in the sub-gap regime of bias voltages. This results in suppression of power dissipation down to a few fW. Low-bias operation of the device with a relatively low probe junction resistance decreases the dissipation by up to two orders of magnitude compared to a conventional device based on an Al-Cu-Al SNS junction and an Al tunnel probe (Al-SQUIPT).

Flux-focusing eddy current probe and rotating probe method for flaw detection

NASA Astrophysics Data System (ADS)

Wincheski, Buzz A.; Fulton, James P.; Nath, Shridhar C.; Simpson, John W.; Namkung, Min

1994-11-01

A flux-focusing electromagnetic sensor which uses a ferromagnetic flux-focusing lens simplifies inspections and increases detectability of fatigue cracks about circular fasteners and other circular inhomogeneities in high conductivity material. The unique feature of the device is the ferrous shield isolating a high-turn pick-up coil from an excitation coil. The use of the magnetic shield is shown to produce a null voltage output across the receiving coil in the presence of an unflawed sample. A redistribution of the current flow in the sample caused by the presence of flaws, however, eliminates the shielding condition and a large output voltage is produced, yielding a clear unambiguous flaw signal. By rotating the probe in a path around a circular fastener such as a rivet while maintaining a constant distance between the probe and the center of a rivet, the signal due to current flow about the rivet can be held constant. Any further changes in the current distribution, such as due to a fatigue crack at the rivet joint, can be detected as an increase in the output voltage above that due to the flow about the rivet head.

Flux-focusing eddy current probe and rotating probe method for flaw detection

NASA Technical Reports Server (NTRS)

Wincheski, Buzz A. (Inventor); Fulton, James P. (Inventor); Nath, Shridhar C. (Inventor); Simpson, John W. (Inventor); Namkung, Min (Inventor)

1994-01-01

A flux-focusing electromagnetic sensor which uses a ferromagnetic flux-focusing lens simplifies inspections and increases detectability of fatigue cracks about circular fasteners and other circular inhomogeneities in high conductivity material. The unique feature of the device is the ferrous shield isolating a high-turn pick-up coil from an excitation coil. The use of the magnetic shield is shown to produce a null voltage output across the receiving coil in the presence of an unflawed sample. A redistribution of the current flow in the sample caused by the presence of flaws, however, eliminates the shielding condition and a large output voltage is produced, yielding a clear unambiguous flaw signal. By rotating the probe in a path around a circular fastener such as a rivet while maintaining a constant distance between the probe and the center of a rivet, the signal due to current flow about the rivet can be held constant. Any further changes in the current distribution, such as due to a fatigue crack at the rivet joint, can be detected as an increase in the output voltage above that due to the flow about the rivet head.

The First Interlaced Continuum Robot, Devised to Intrinsically Follow the Leader

PubMed Central

Kang, Byungjeon; Kojcev, Risto; Sinibaldi, Edoardo

2016-01-01

Flexible probes that are safely deployed to hard-to-reach targets while avoiding critical structures are strategic in several high-impact application fields, including the biomedical sector and the sector of inspections at large. A critical problem for these tools is the best approach for deploying an entire tool body, not only its tip, on a sought trajectory. A probe that achieves this deployment is considered to follow the leader (or to achieve follow-the-leader deployment) because its body sections follow the track traced by its tip. Follow-the-leader deployment through cavities is complicated due to a lack of external supports. Currently, no definitive implementation for a probe that is intrinsically able to follow the leader, i.e., without relying on external supports, has been achieved. In this paper, we present a completely new device, namely the first interlaced continuum robot, devised to intrinsically follow the leader. We developed the interlaced configuration by pursuing a conceptual approach irrespective of application-specific constraints and assuming two flexible tools with controllable stiffness. We questioned the possibility of solving the previously mentioned deployment problem by harnessing probe symmetry during the design process. This study examines the entire development of the novel interlaced probe: model-based conceptual design, detailed design and prototyping, and preliminary experimental assessment. Our probe can build a track with a radius of curvature that is as small as twice the probe diameter, which enables it to outperform state-of-the-art tools that are aimed at follow-the-leader deployment. Despite the limitations that are inherently associated with its original character, this study provides a prototypical approach to the design of interlaced continuum systems and demonstrates the first interlaced continuum probe, which is intrinsically able to follow the leader. PMID:26914328

The First Interlaced Continuum Robot, Devised to Intrinsically Follow the Leader.

PubMed

Kang, Byungjeon; Kojcev, Risto; Sinibaldi, Edoardo

2016-01-01

Flexible probes that are safely deployed to hard-to-reach targets while avoiding critical structures are strategic in several high-impact application fields, including the biomedical sector and the sector of inspections at large. A critical problem for these tools is the best approach for deploying an entire tool body, not only its tip, on a sought trajectory. A probe that achieves this deployment is considered to follow the leader (or to achieve follow-the-leader deployment) because its body sections follow the track traced by its tip. Follow-the-leader deployment through cavities is complicated due to a lack of external supports. Currently, no definitive implementation for a probe that is intrinsically able to follow the leader, i.e., without relying on external supports, has been achieved. In this paper, we present a completely new device, namely the first interlaced continuum robot, devised to intrinsically follow the leader. We developed the interlaced configuration by pursuing a conceptual approach irrespective of application-specific constraints and assuming two flexible tools with controllable stiffness. We questioned the possibility of solving the previously mentioned deployment problem by harnessing probe symmetry during the design process. This study examines the entire development of the novel interlaced probe: model-based conceptual design, detailed design and prototyping, and preliminary experimental assessment. Our probe can build a track with a radius of curvature that is as small as twice the probe diameter, which enables it to outperform state-of-the-art tools that are aimed at follow-the-leader deployment. Despite the limitations that are inherently associated with its original character, this study provides a prototypical approach to the design of interlaced continuum systems and demonstrates the first interlaced continuum probe, which is intrinsically able to follow the leader.

Biomedical Probes Based on Inorganic Nanoparticles for Electrochemical and Optical Spectroscopy Applications

PubMed Central

Yakoh, Abdulhadee; Pinyorospathum, Chanika; Siangproh, Weena; Chailapakul, Orawon

2015-01-01

Inorganic nanoparticles usually provide novel and unique physical properties as their size approaches nanometer scale dimensions. The unique physical and optical properties of nanoparticles may lead to applications in a variety of areas, including biomedical detection. Therefore, current research is now increasingly focused on the use of the high surface-to-volume ratios of nanoparticles to fabricate superb chemical- or biosensors for various detection applications. This article highlights various kinds of inorganic nanoparticles, including metal nanoparticles, magnetic nanoparticles, nanocomposites, and semiconductor nanoparticles that can be perceived as useful materials for biomedical probes and points to the outstanding results arising from their use in such probes. The progress in the use of inorganic nanoparticle-based electrochemical, colorimetric and spectrophotometric detection in recent applications, especially bioanalysis, and the main functions of inorganic nanoparticles in detection are reviewed. The article begins with a conceptual discussion of nanoparticles according to types, followed by numerous applications to analytes including biomolecules, disease markers, and pharmaceutical substances. Most of the references cited herein, dating from 2010 to 2015, generally mention one or more of the following characteristics: a low detection limit, good signal amplification and simultaneous detection capabilities. PMID:26343676

Comparison of Metal-Backed Free-Space and Open-Ended Coaxial Probe Techniques for the Dielectric Characterization of Aeronautical Composites †

PubMed Central

López-Rodríguez, Patricia; Escot-Bocanegra, David; Poyatos-Martínez, David; Weinmann, Frank

2016-01-01

The trend in the last few decades is that current unmanned aerial vehicles are completely made of composite materials rather than metallic, such as carbon-fiber or fiberglass composites. From the electromagnetic point of view, this fact forces engineers and scientists to assess how these materials may affect their radar response or their electronics in terms of electromagnetic compatibility. In order to evaluate this, electromagnetic characterization of different composite materials has become a need. Several techniques exist to perform this characterization, all of them based on the utilization of different sensors for measuring different parameters. In this paper, an implementation of the metal-backed free-space technique, based on the employment of antenna probes, is utilized for the characterization of composite materials that belong to an actual drone. Their extracted properties are compared with those given by a commercial solution, an open-ended coaxial probe (OECP). The discrepancies found between both techniques along with a further evaluation of the methodologies, including measurements with a split-cavity resonator, conclude that the implemented free-space technique provides more reliable results for this kind of composites than the OECP technique. PMID:27347966

Fast-Response Turn-on Fluorescent Probes Based on Thiolysis of NBD Amine for H2 S Bioimaging.

PubMed

Wang, Runyu; Li, Zhifei; Zhang, Changyu; Li, Yanyan; Xu, Guoce; Zhang, Qiang-Zhe; Li, Lu-Yuan; Yi, Long; Xi, Zhen

2016-05-17

Hydrogen sulfide (H2 S) is an important endogenous signaling molecule with multiple biological functions. New selective fluorescent turn-on probes based on fast thiolyling of NBD (7-nitro-1,2,3-benzoxadiazole) amine were explored for sensing H2 S in aqueous buffer and in living cells. The syntheses of both probes are simple and quite straightforward. The probes are highly sensitive and selective toward H2 S over other biologically relevant species. The fluorescein-NBD-based probe showed 65-fold green fluorescent increase upon H2 S activation. The rhodamine-NBD-based probe reacted rapidly with H2 S (t1/2 ≈1 min) to give a 4.5-fold increase in red fluorescence. Moreover, both probes were successfully used for monitoring H2 S in living cells and in mice. Based on such probe-based tools, we could observe H2 O2 -induced H2 S biogenesis in a concentration-dependent and time-dependent fashion in living cells. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Electron-beam-induced-current and active secondary-electron voltage-contrast with aberration-corrected electron probes

DOE PAGES

Han, Myung-Geun; Garlow, Joseph A.; Marshall, Matthew S. J.; ...

2017-03-23

The ability to map out electrostatic potentials in materials is critical for the development and the design of nanoscale electronic and spintronic devices in modern industry. Electron holography has been an important tool for revealing electric and magnetic field distributions in microelectronics and magnetic-based memory devices, however, its utility is hindered by several practical constraints, such as charging artifacts and limitations in sensitivity and in field of view. In this article, we report electron-beam-induced-current (EBIC) and secondary-electron voltage-contrast (SE-VC) with an aberration-corrected electron probe in a transmission electron microscope (TEM), as complementary techniques to electron holography, to measure electric fieldsmore » and surface potentials, respectively. These two techniques were applied to ferroelectric thin films, multiferroic nanowires, and single crystals. Electrostatic potential maps obtained by off-axis electron holography were compared with EBIC and SE-VC to show that these techniques can be used as a complementary approach to validate quantitative results obtained from electron holography analysis.« less

An H₂S Sensor Based on Electrochemistry for Chicken Coops.

PubMed

Zeng, Lihua; He, Mei; Yu, Huihui; Li, Daoliang

2016-08-31

The recent modernization of the livestock industry lags behind the scale of the livestock industry, particularly in indoor environmental monitoring. In particular, the H₂S gas concentration in chicken coops affects the growth and reproductive capacity of the chickens and threatens their health. Therefore, the research and development of a low-cost, environmentally friendly sensor that can achieve on-line monitoring of H₂S gas has a notably important practical significance. This paper reports the design of an H₂S gas sensor, with selection of an electrochemical probe with high accuracy and wide measurement range using the relatively mature technology of electrochemical sensors. Although the probe of the sensor is the main factor that affects the sensor accuracy, the probe must be combined with a specifically designed signal condition circuit that can overcome the lack of an electrode to satisfy the requirements for the interconnection and matching between the output signal and the test instrument. Because the output current of the electrochemical electrode is small and likely to be disturbed by noise, we designed signal-conditioning modules. Through the signal-conditioning circuit, the output signal of the current electrode can be converted into a voltage and amplified. In addition, we designed a power control module because a bias voltage is necessary for the electrode. Finally, after the calibration experiment, the accurate concentration of H₂S gas can be measured. Based on the experimental analysis, the sensor shows good linearity and selectivity, comparatively high sensitivity, perfect stability and an extremely long operating life of up to two years.

Nonpeptide-Based Small-Molecule Probe for Fluorogenic and Chromogenic Detection of Chymotrypsin.

PubMed

Wu, Lei; Yang, Shu-Hou; Xiong, Hao; Yang, Jia-Qian; Guo, Jun; Yang, Wen-Chao; Yang, Guang-Fu

2017-03-21

We report herein a nonpeptide-based small-molecule probe for fluorogenic and chromogenic detection of chymotrypsin, as well as the primary application for this probe. This probe was rationally designed by mimicking the peptide substrate and optimized by adjusting the recognition group. The refined probe 2 exhibits good specificity toward chymotrypsin, producing about 25-fold higher enhancement in both the fluorescence intensity and absorbance upon the catalysis by chymotrypsin. Compared with the most widely used peptide substrate (AMC-FPAA-Suc) of chymotrypsin, probe 2 shows about 5-fold higher binding affinity and comparable catalytical efficiency against chymotrypsin. Furthermore, it was successfully applied for the inhibitor characterization. To the best of our knowledge, probe 2 is the first nonpeptide-based small-molecule probe for chymotrypsin, with the advantages of simple structure and high sensitivity compared to the widely used peptide-based substrates. This small-molecule probe is expected to be a useful molecular tool for drug discovery and chymotrypsin-related disease diagnosis.

Fluorescent Probes and Selective Inhibitors for Biological Studies of Hydrogen Sulfide- and Polysulfide-Mediated Signaling.

PubMed

Takano, Yoko; Echizen, Honami; Hanaoka, Kenjiro

2017-10-01

Hydrogen sulfide (H 2 S) plays roles in many physiological processes, including relaxation of vascular smooth muscles, mediation of neurotransmission, inhibition of insulin signaling, and regulation of inflammation. Also, hydropersulfide (R-S-SH) and polysulfide (-S-S n -S-) have recently been identified as reactive sulfur species (RSS) that regulate the bioactivities of multiple proteins via S-sulfhydration of cysteine residues (protein Cys-SSH) and show cytoprotection. Chemical tools such as fluorescent probes and selective inhibitors are needed to establish in detail the physiological roles of H 2 S and polysulfide. Recent Advances: Although many fluorescent probes for H 2 S are available, fluorescent probes for hydropersulfide and polysulfide have only recently been developed and used to detect these sulfur species in living cells. In this review, we summarize recent progress in developing chemical tools for the study of H 2 S, hydropersulfide, and polysulfide, covering fluorescent probes based on various design strategies and selective inhibitors of H 2 S- and polysulfide-producing enzymes (cystathionine γ-lyase, cystathionine β-synthase, and 3-mercaptopyruvate sulfurtransferase), and we summarize their applications in biological studies. Despite recent progress, the precise biological functions of H 2 S, hydropersulfide, and polysulfide remain to be fully established. Fluorescent probes and selective inhibitors are effective chemical tools to study the physiological roles of these sulfur molecules in living cells and tissues. Therefore, further development of a broad range of practical fluorescent probes and selective inhibitors as tools for studies of RSS biology is currently attracting great interest. Antioxid. Redox Signal. 27, 669-683.

RF current sensor

DOEpatents

Moore, James A.; Sparks, Dennis O.

1998-11-10

An RF sensor having a novel current sensing probe and a voltage sensing probe to measure voltage and current. The current sensor is disposed in a transmission line to link all of the flux generated by the flowing current in order to obtain an accurate measurement. The voltage sensor is a flat plate which operates as a capacitive plate to sense voltage on a center conductor of the transmission line, in which the measured voltage is obtained across a resistance leg of a R-C differentiator circuit formed by the characteristic impedance of a connecting transmission line and a capacitance of the plate, which is positioned proximal to the center conductor.

Propagation of Dipolarization Signatures Observed by the Van Allen Probes in the Inner Magnetosphere

NASA Astrophysics Data System (ADS)

Ohtani, S.; Motoba, T.; Gkioulidou, M.; Takahashi, K.; Kletzing, C.

2017-12-01

Dipolarization, the change of the local magnetic field from a stretched to a more dipolar configuration, is one of the most fundamental processes of magnetospheric physics. It is especially critical for the dynamics of the inner magnetosphere. The associated electric field accelerates ions and electrons and transports them closer to Earth. Such injected ions intensify the ring current, and electrons constitute the seed population of the radiation belt. Those ions and electrons may also excite various waves that play important roles in the enhancement and loss of the radiation belt electrons. Despite such critical consequences, the general characteristics of dipolarization in the inner magnetosphere still remain to be understood. The Van Allen Probes mission, which consists of two probes that orbit through the equatorial region of the inner magnetosphere, provides an ideal opportunity to examine dipolarization signatures in the core of the ring current. In the present study we investigate the spatial expansion of the dipolarization region by examining the correlation and time delay of dipolarization signatures observed by the two probes. Whereas in general it requires three-point measurements to deduce the propagation of a signal on a certain plane, we statically examined the observed time delays and found that dipolarization signatures tend to propagate radially inward as well as away from midnight. In this paper we address the propagation of dipolarization signatures quantitatively and compare with the propagation velocities reported previously based on observations made farther away from Earth. We also discuss how often and under what conditions the dipolarization region expands.

Four-probe measurements with a three-probe scanning tunneling microscope.

PubMed

Salomons, Mark; Martins, Bruno V C; Zikovsky, Janik; Wolkow, Robert A

2014-04-01

We present an ultrahigh vacuum (UHV) three-probe scanning tunneling microscope in which each probe is capable of atomic resolution. A UHV JEOL scanning electron microscope aids in the placement of the probes on the sample. The machine also has a field ion microscope to clean, atomically image, and shape the probe tips. The machine uses bare conductive samples and tips with a homebuilt set of pliers for heating and loading. Automated feedback controlled tip-surface contacts allow for electrical stability and reproducibility while also greatly reducing tip and surface damage due to contact formation. The ability to register inter-tip position by imaging of a single surface feature by multiple tips is demonstrated. Four-probe material characterization is achieved by deploying two tips as fixed current probes and the third tip as a movable voltage probe.

21 CFR 884.4150 - Bipolar endoscopic coagulator-cutter and accessories.

Code of Federal Regulations, 2014 CFR

2014-04-01

... high frequency electrical current through tissue between two electrical contacts of a probe. This generic type of device may include the following accessories: an electrical generator, probes, and...

21 CFR 884.4150 - Bipolar endoscopic coagulator-cutter and accessories.

Code of Federal Regulations, 2012 CFR

2012-04-01

... high frequency electrical current through tissue between two electrical contacts of a probe. This generic type of device may include the following accessories: an electrical generator, probes, and...

21 CFR 884.4150 - Bipolar endoscopic coagulator-cutter and accessories.

Code of Federal Regulations, 2011 CFR

2011-04-01

... high frequency electrical current through tissue between two electrical contacts of a probe. This generic type of device may include the following accessories: an electrical generator, probes, and...

21 CFR 884.4150 - Bipolar endoscopic coagulator-cutter and accessories.

Code of Federal Regulations, 2013 CFR

2013-04-01

... high frequency electrical current through tissue between two electrical contacts of a probe. This generic type of device may include the following accessories: an electrical generator, probes, and...

A versatile nondestructive evaluation imaging workstation

NASA Technical Reports Server (NTRS)

Chern, E. James; Butler, David W.

1994-01-01

Ultrasonic C-scan and eddy current imaging systems are of the pointwise type evaluation systems that rely on a mechanical scanner to physically maneuver a probe relative to the specimen point by point in order to acquire data and generate images. Since the ultrasonic C-scan and eddy current imaging systems are based on the same mechanical scanning mechanisms, the two systems can be combined using the same PC platform with a common mechanical manipulation subsystem and integrated data acquisition software. Based on this concept, we have developed an IBM PC-based combined ultrasonic C-scan and eddy current imaging system. The system is modularized and provides capacity for future hardware and software expansions. Advantages associated with the combined system are: (1) eliminated duplication of the computer and mechanical hardware, (2) unified data acquisition, processing and storage software, (3) reduced setup time for repetitious ultrasonic and eddy current scans, and (4) improved system efficiency. The concept can be adapted to many engineering systems by integrating related PC-based instruments into one multipurpose workstation such as dispensing, machining, packaging, sorting, and other industrial applications.

A versatile nondestructive evaluation imaging workstation

NASA Astrophysics Data System (ADS)

Chern, E. James; Butler, David W.

1994-02-01

Ultrasonic C-scan and eddy current imaging systems are of the pointwise type evaluation systems that rely on a mechanical scanner to physically maneuver a probe relative to the specimen point by point in order to acquire data and generate images. Since the ultrasonic C-scan and eddy current imaging systems are based on the same mechanical scanning mechanisms, the two systems can be combined using the same PC platform with a common mechanical manipulation subsystem and integrated data acquisition software. Based on this concept, we have developed an IBM PC-based combined ultrasonic C-scan and eddy current imaging system. The system is modularized and provides capacity for future hardware and software expansions. Advantages associated with the combined system are: (1) eliminated duplication of the computer and mechanical hardware, (2) unified data acquisition, processing and storage software, (3) reduced setup time for repetitious ultrasonic and eddy current scans, and (4) improved system efficiency. The concept can be adapted to many engineering systems by integrating related PC-based instruments into one multipurpose workstation such as dispensing, machining, packaging, sorting, and other industrial applications.

Optical Probes for Neurobiological Sensing and Imaging.

PubMed

Kim, Eric H; Chin, Gregory; Rong, Guoxin; Poskanzer, Kira E; Clark, Heather A

2018-05-15

Fluorescent nanosensors and molecular probes are next-generation tools for imaging chemical signaling inside and between cells. Electrophysiology has long been considered the gold standard in elucidating neural dynamics with high temporal resolution and precision, particularly on the single-cell level. However, electrode-based techniques face challenges in illuminating the specific chemicals involved in neural cell activation with adequate spatial information. Measuring chemical dynamics is of fundamental importance to better understand synergistic interactions between neurons as well as interactions between neurons and non-neuronal cells. Over the past decade, significant technological advances in optical probes and imaging methods have enabled entirely new possibilities for studying neural cells and circuits at the chemical level. These optical imaging modalities have shown promise for combining chemical, temporal, and spatial information. This potential makes them ideal candidates to unravel the complex neural interactions at multiple scales in the brain, which could be complemented by traditional electrophysiological methods to obtain a full spatiotemporal picture of neurochemical dynamics. Despite the potential, only a handful of probe candidates have been utilized to provide detailed chemical information in the brain. To date, most live imaging and chemical mapping studies rely on fluorescent molecular indicators to report intracellular calcium (Ca 2+ ) dynamics, which correlates with neuronal activity. Methodological advances for monitoring a full array of chemicals in the brain with improved spatial, temporal, and chemical resolution will thus enable mapping of neurochemical circuits with finer precision. On the basis of numerous studies in this exciting field, we review the current efforts to develop and apply a palette of optical probes and nanosensors for chemical sensing in the brain. There is a strong impetus to further develop technologies capable of probing entire neurobiological units with high spatiotemporal resolution. Thus, we introduce selected applications for ion and neurotransmitter detection to investigate both neurons and non-neuronal brain cells. We focus on families of optical probes because of their ability to sense a wide array of molecules and convey spatial information with minimal damage to tissue. We start with a discussion of currently available molecular probes, highlight recent advances in genetically modified fluorescent probes for ions and small molecules, and end with the latest research in nanosensors for biological imaging. Customizable, nanoscale optical sensors that accurately and dynamically monitor the local environment with high spatiotemporal resolution could lead to not only new insights into the function of all cell types but also a broader understanding of how diverse neural signaling systems act in conjunction with neighboring cells in a spatially relevant manner.

A Thermal Melt Probe System for Extensive, Low-Cost Instrument Deployment Within and Beneath Ice Sheets

NASA Astrophysics Data System (ADS)

Winebrenner, D. P.; Elam, W. T.; Carpenter, M.; Kintner, P., III

2014-12-01

More numerous observations within and beneath ice sheets are needed to address a broad variety of important questions concerning ice sheets and climate. However, emplacement of instruments continues to be constrained by logistical burdens, especially in cold ice a kilometer or more thick. Electrically powered thermal melt probes are inherently logistically light and efficient, especially for reaching greater depths in colder ice. They therefore offer a means of addressing current measurement problems, but have been limited historically by a lack of technology for reliable operation at the necessary voltages and powers. Here we report field tests in Greenland of two new melt probes. We operated one probe at 2.2 kilowatts (kW) and 1050 volts (V), achieving a depth of 400 m in the ice in ~ 120 hours, without electrical failure. That depth is the second greatest achieved thus far with a thermal melt probe, exceeded only by one deployment to 1005 m in Greenland in 1968, which ended in an electrical failure. Our test run took place in two intervals separated by a year, with the probe frozen at 65 m depth during the interim, after which we re-established communication, unfroze the probe, and proceeded to the greater depth. During the second field test we operated a higher-power probe, initially at 2.5 kW and 1500 V and progressing to 4.5 kW and 2000 V. Initial data indicate that this probe achieved a descent rate of 8 m/hr, which if correct would be the fastest rate yet achieved for such probes. Moreover, we observed maintenance of vertical probe travel using pendulum steering throughout both tests, as well as autonomous descent without operator-intervention after launch. The latter suggests potential for crews of 1-2 to operate several melt probes concurrently. However, the higher power probe did suffer electrical failure of a heating element after 7 hours of operation at 2000 V (24 hours after the start of the test), contrary to expectations based on laboratory component and system testing. We are therefore revising the probe heaters using a newer but more development-intensive technology. With probe systems now validated in our tests, this will result in a reliable means to emplace instruments for studies of subglacial hydrology, ice dynamics, and possible subglacial ecologies.

The magnetospheric disturbance ring current as a source for probing the deep earth electrical conductivity

USGS Publications Warehouse

Campbell, W.H.

1990-01-01

Two current rings have been observed in the equatorial plane of the earth at times of high geomagnetic activity. An eastward current exists between about 2 and 3.5 earth radii (Re) distant, and a larger, more variable companion current exists between about 4 and 9 Re. These current regions are loaded during geomagnetic substorms. They decay, almost exponentially, after the cessation of the particle influx that attends the solar wind disturbance. This review focuses upon characteristics needed for intelligent use of the ring current as a source for induction probing of the earth's mantle. Considerable difficulties are found with the assumption that Dst is a ring-current index. ?? 1990 Birkha??user Verlag.

Method and apparatus for correcting eddy current signal voltage for temperature effects

DOEpatents

Kustra, Thomas A.; Caffarel, Alfred J.

1990-01-01

An apparatus and method for measuring physical characteristics of an electrically conductive material by the use of eddy-current techniques and compensating measurement errors caused by changes in temperature includes a switching arrangement connected between primary and reference coils of an eddy-current probe which allows the probe to be selectively connected between an eddy current output oscilloscope and a digital ohm-meter for measuring the resistances of the primary and reference coils substantially at the time of eddy current measurement. In this way, changes in resistance due to temperature effects can be completely taken into account in determining the true error in the eddy current measurement. The true error can consequently be converted into an equivalent eddy current measurement correction.

Storm- Time Dynamics of Ring Current Protons: Implications for the Long-Term Energy Budget in the Inner Magnetosphere.

NASA Astrophysics Data System (ADS)

Gkioulidou, M.; Ukhorskiy, A. Y.; Mitchell, D. G.; Lanzerotti, L. J.

2015-12-01

The ring current energy budget plays a key role in the global electrodynamics of Earth's space environment. Pressure gradients developed in the inner magnetosphere can shield the near-Earth region from solar wind-induced electric fields. The distortion of Earth's magnetic field due to the ring current affects the dynamics of particles contributing both to the ring current and radiation belts. Therefore, understanding the long-term evolution of the inner magnetosphere energy content is essential. We have investigated the evolution of ring current proton pressure (7 - 600 keV) in the inner magnetosphere based on data from the Radiation Belt Storm Probes Ion Composition Experiment (RBSPICE) instrument aboard Van Allen Probe B throughout the year 2013. We find that although the low-energy component of the protons (< 80 keV) is governed by convective timescales and is very well correlated with the Dst index, the high-energy component (>100 keV) varies on much longer timescales and shows either no or anti-correlation with the Dst index. Interestingly, the contributions of the high- and low-energy protons to the total energy content are comparable. Our results indicate that the proton dynamics, and as a consequence the total energy budget in the inner magnetosphere (inside geosynchronous orbit), is not strictly controlled by storm-time timescales as those are defined by the Dst index.

Eddy-Current Monitoring Of Composite Layups

NASA Technical Reports Server (NTRS)

Fox, Robert L.; Buckley, John D.

1993-01-01

Eddy-current-probe apparatus used to determine predominant orientations of fibers in fiber/matrix composite materials. Apparatus nondestructive, noninvasive means for monitoring composite prepregs and layups during fabrication to ensure predictable and repeatable mechanical properties of finished composite panels. Consists essentially of electromagnet coil wrapped around horseshoe-shaped powdered-iron or ferrite ore. Optionally, capacitor included in series or parallel with coil to form resonant circuit. Impedance monitor excites radio-frequency current in coil and measures impedance of probe circuit. Affected by whatever material placed near ends of core, where material intercepts alternating magnetic field excited in core by current in coil.

Selection of turning-on fluorogenic probe as protein-specific detector obtained via the 10BASEd-T

NASA Astrophysics Data System (ADS)

Uematsu, Shuta; Midorikawa, Taiki; Ito, Yuji; Taki, Masumi

2017-01-01

In order to obtain a molecular probe for specific protein detection, we have synthesized fluorogenic probe library of vast diversity on bacteriophage T7 via the gp10 based-thioetherification (10BASEd-T). A remarkable turning- on probe which is excitable by widely applicable visible light was selected from the library.

Fast capacitive probe for electromagnetic pulse diagnostic.

PubMed

Lorusso, A; Nassisi, V; Siciliano, M V

2008-06-01

In this work, we report the study and the development of a capacitive probe which is suitable for getting fast and high voltage/current measurements. Due to the fact that fast pulses propagate generally in coaxial structures, the probe realized in this work was a capacitive divider with the divider electrode properly designed to assure the same characteristic impedance of the coaxial structure and the recombination time of the split signals during the propagation. It was a folded cylindrical ring of 1.4 cm long and 0.8 cm thick, which introduce a theoretical delay time of about 100 ps. Analyzing the behavior of the probe closed on 520 Omega, the voltage amplification resulted to be of (3.6+/-0.1) x 10(-4) and, as a consequence, the current attenuation factor of 56+/-1 AV. The response rise time was less than 320 ps, which was limited by oscilloscope bandwave. The capacitor probe can operate voltage measurements of the order of 100 kV.

Removal of singularity in radial Langmuir probe models for non-zero ion temperature

NASA Astrophysics Data System (ADS)

Regodón, Guillermo Fernando; Fernández Palop, José Ignacio; Tejero-del-Caz, Antonio; Díaz-Cabrera, Juan Manuel; Carmona-Cabezas, Rafael; Ballesteros, Jerónimo

2017-10-01

We solve a radial theoretical model that describes the ion sheath around a cylindrical Langmuir probe with finite non-zero ion temperature in which singularity in an a priori unknown point prevents direct integration. The singularity appears naturally in fluid models when the velocity of the ions reaches the local ion speed of sound. The solutions are smooth and continuous and are valid from the plasma to the probe with no need for asymptotic matching. The solutions that we present are valid for any value of the positive ion to electron temperature ratio and for any constant polytropic coefficient. The model is numerically solved to obtain the electric potential and the ion population density profiles for any given positive ion current collected by the probe. The ion-current to probe-voltage characteristic curves and the Sonin plot are calculated in order to use the results of the model in plasma diagnosis. The proposed methodology is adaptable to other geometries and in the presence of other presheath mechanisms.

Electromagnetic microscope compared with a conventional pulsed eddy-current probe

NASA Astrophysics Data System (ADS)

Podney, Walter N.

1998-03-01

A superconductive probe presently can detect a crack at a rivet hole that is two to three times smaller than the smallest crack detectable by a conventional probe. As the technology matures and noise resolution approaches a limit set by SQUIDS, approximately 1 fH, it will enable detecting submillimeter cracks down to approximately 15 mm.

Giant Magnetoresistance Sensors: A Review on Structures and Non-Destructive Eddy Current Testing Applications

PubMed Central

Rifai, Damhuji; Abdalla, Ahmed N.; Ali, Kharudin; Razali, Ramdan

2016-01-01

Non-destructive eddy current testing (ECT) is widely used to examine structural defects in ferromagnetic pipe in the oil and gas industry. Implementation of giant magnetoresistance (GMR) sensors as magnetic field sensors to detect the changes of magnetic field continuity have increased the sensitivity of eddy current techniques in detecting the material defect profile. However, not many researchers have described in detail the structure and issues of GMR sensors and their application in eddy current techniques for nondestructive testing. This paper will describe the implementation of GMR sensors in non-destructive testing eddy current testing. The first part of this paper will describe the structure and principles of GMR sensors. The second part outlines the principles and types of eddy current testing probe that have been studied and developed by previous researchers. The influence of various parameters on the GMR measurement and a factor affecting in eddy current testing will be described in detail in the third part of this paper. Finally, this paper will discuss the limitations of coil probe and compensation techniques that researchers have applied in eddy current testing probes. A comprehensive review of previous studies on the application of GMR sensors in non-destructive eddy current testing also be given at the end of this paper. PMID:26927123

Giant Magnetoresistance Sensors: A Review on Structures and Non-Destructive Eddy Current Testing Applications.

PubMed

Rifai, Damhuji; Abdalla, Ahmed N; Ali, Kharudin; Razali, Ramdan

2016-02-26

Non-destructive eddy current testing (ECT) is widely used to examine structural defects in ferromagnetic pipe in the oil and gas industry. Implementation of giant magnetoresistance (GMR) sensors as magnetic field sensors to detect the changes of magnetic field continuity have increased the sensitivity of eddy current techniques in detecting the material defect profile. However, not many researchers have described in detail the structure and issues of GMR sensors and their application in eddy current techniques for nondestructive testing. This paper will describe the implementation of GMR sensors in non-destructive testing eddy current testing. The first part of this paper will describe the structure and principles of GMR sensors. The second part outlines the principles and types of eddy current testing probe that have been studied and developed by previous researchers. The influence of various parameters on the GMR measurement and a factor affecting in eddy current testing will be described in detail in the third part of this paper. Finally, this paper will discuss the limitations of coil probe and compensation techniques that researchers have applied in eddy current testing probes. A comprehensive review of previous studies on the application of GMR sensors in non-destructive eddy current testing also be given at the end of this paper.

Measurement of vehicle potential using a mother-daughter tethered rocket

NASA Technical Reports Server (NTRS)

Williamson, P. R.; Denig, W. F.; Banks, P. M.; Raitt, W. J.; Kawashima, N.; Hirao, K.; Oyama, K. I.; Sasaki, S.

1982-01-01

The equipment, experimental design, and results of mother-daughter tethered probes for measuring the potential of a spacecraft are described. The object was to inject a probe into the ionosphere by rocket and then lower an impedance voltage monitor-equipped section of the probe by means of a highly insulated wire. The mother probe, also carrying voltage monitors, would inject charges into the plasma that would be measured at both ends of the tether. Instrumentation on the daughter probe included voltage current monitors and a Langmuir probe, while the mother payload also carried a charge probe, floating probe, a Langmuir probe, and an impedance probe. The first launch was from Japan in 1980, and operations confirmed that Langmuir probes with area ratios less than 400:1 can produce changes in the vehicle potential if probe voltages of more than 10 V are applied in the collection mode. A ratio of 200:1 was sufficient for the daughter probe with voltages of 5 V. The experiment is concluded to verify the tethered probe method of measuring vehicle potential.

Corrosion probe. Innovative technology summary report

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

Over 253 million liters of high-level waste (HLW) generated from plutonium production is stored in mild steel tanks at the Department of Energy (DOE) Hanford Site. Corrosion monitoring of double-shell storage tanks (DSTs) is currently performed at Hanford using a combination of process knowledge and tank waste sampling and analysis. Available technologies for corrosion monitoring have progressed to a point where it is feasible to monitor and control corrosion by on-line monitoring of the corrosion process and direct addition of corrosion inhibitors. The electrochemical noise (EN) technique deploys EN-based corrosion monitoring probes into storage tanks. This system is specifically designedmore » to measure corrosion rates and detect changes in waste chemistry that trigger the onset of pitting and cracking. These on-line probes can determine whether additional corrosion inhibitor is required and, if so, provide information on an effective end point to the corrosion inhibitor addition procedure. This report describes the technology, its performance, its application, costs, regulatory and policy issues, and lessons learned.« less

Microfluidics for Positron Emission Tomography (PET) Imaging Probe Development

PubMed Central

Wang, Ming-Wei; Lin, Wei-Yu; Liu, Kan; Masterman-Smith, Michael; Shen, Clifton Kwang-Fu

2012-01-01

Due to increased needs for Positron Emission Tomography (PET) scanning, high demands for a wide variety of radiolabeled compounds will have to be met by exploiting novel radiochemistry and engineering technologies to improve the production and development of PET probes. The application of microfluidic reactors to perform radiosyntheses is currently attracting a great deal of interest because of their potential to deliver many advantages over conventional labeling systems. Microfluidic-based radiochemistry can lead to the use of smaller quantities of precursors, accelerated reaction rates and easier purification processes with greater yield and higher specific activity of desired probes. Several ‘proof-of-principle’ examples, along with basics of device architecture and operation, and potential limitations of each design are discussed here. Along with the concept of radioisotope distribution from centralized cyclotron facilities to individual imaging centers and laboratories (“decentralized model”), an easy-to-use, standalone, flexible, fully-automated radiochemical microfluidic platform can open up to simpler and more cost-effective procedures for molecular imaging using PET. PMID:20643021

Modified surface of titanium dioxide nanoparticles-based biosensor for DNA detection

NASA Astrophysics Data System (ADS)

Nadzirah, Sh.; Hashim, U.; Rusop, M.

2018-05-01

A new technique was used to develop a simple and selective picoammeter DNA biosensor for identification of E. coli O157:H7. This biosensor was fabricated from titanium dioxide nanoparticles that was synthesized by sol-gel method and spin-coated on silicon dioxide substrate via spinner. 3-Aminopropyl triethoxy silane (APTES) was used to modify the surface of TiO2. Simple surface modification approach has been applied; which is single dropping of APTES onto the TiO2 nanoparticles surface. Carboxyl modified probe DNA has been bind onto the surface of APTES/TiO2 without any amplifier element. Electrical signal has been used as the indicator to differentiate each step (surface modification of TiO2 and probe DNA immobilization). The I-V measurements indicate extremely low current (pico-ampere) flow through the device which is 2.8138E-10 A for pure TiO2 nanoparticles, 2.8124E-10 A after APTES modification and 3.5949E-10 A after probe DNA immobilization.

Effect of different concentration of HPV DNA probe immobilization for cervical cancer detection based IDE biosensor

NASA Astrophysics Data System (ADS)

Roshila, M. L.; Hashim, U.; Azizah, N.; Nadzirah, Sh.; Arshad, M. K. Md; Ruslinda, A. R.; Gopinath, Subash C. B.

2017-03-01

This paper principally delineates to the detection process of Human Papillomavirus (HPV) DNA test. HPV is an extremely common virus infection that infected to human by the progressions cell in the cervix cell. The types of HPV that give a most exceedingly awful infected with cervical cancer is 16 and 18 other than 31 and 45. The HPV DNA probe is immobilized with a different concentration to stabilize the sensitivity. A technique of rapid and sensitive for the HPV identification was proposed by coordinating basic DNA extraction with a quality of DNA. The extraction of the quality of DNA will make a proficiency of the discovery procedure. It will rely on the sequence of the capture probes and the way to support their attached. The fabrication, surface modification, immobilization and hybridization procedures are described by current-voltage (I-V) estimation by utilizing KEITHLEY 6487. This procedure will play out a decent affectability and selectivity of HPV discovery.

Advance in multi-hit detection and quantization in atom probe tomography.

PubMed

Da Costa, G; Wang, H; Duguay, S; Bostel, A; Blavette, D; Deconihout, B

2012-12-01

The preferential retention of high evaporation field chemical species at the sample surface in atom-probe tomography (e.g., boron in silicon or in metallic alloys) leads to correlated field evaporation and pronounced pile-up effects on the detector. The latter severely affects the reliability of concentration measurements of current 3D atom probes leading to an under-estimation of the concentrations of the high-field species. The multi-hit capabilities of the position-sensitive time-resolved detector is shown to play a key role. An innovative method based on Fourier space signal processing of signals supplied by an advance delay-line position-sensitive detector is shown to drastically improve the time resolving power of the detector and consequently its capability to detect multiple events. Results show that up to 30 ions on the same evaporation pulse can be detected and properly positioned. The major impact of this new method on the quantization of chemical composition in materials, particularly in highly-doped Si(B) samples is highlighted.

Probing Extreme-density Matter with Gravitational-wave Observations of Binary Neutron Star Merger Remnants

DOE Office of Scientific and Technical Information (OSTI.GOV)

Radice, David; Bernuzzi, Sebastiano; Pozzo, Walter Del

We present a proof-of-concept study, based on numerical-relativity simulations, of how gravitational waves (GWs) from neutron star merger remnants can probe the nature of matter at extreme densities. Phase transitions and extra degrees of freedom can emerge at densities beyond those reached during the inspiral, and typically result in a softening of the equation of state (EOS). We show that such physical effects change the qualitative dynamics of the remnant evolution, but they are not identifiable as a signature in the GW frequency, with the exception of possible black hole formation effects. The EOS softening is, instead, encoded in themore » GW luminosity and phase and is in principle detectable up to distances of the order of several megaparsecs with advanced detectors and up to hundreds of megaparsecs with third-generation detectors. Probing extreme-density matter will require going beyond the current paradigm and developing a more holistic strategy for modeling and analyzing postmerger GW signals.« less

GAIA: A WINDOW TO LARGE-SCALE MOTIONS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nusser, Adi; Branchini, Enzo; Davis, Marc, E-mail: adi@physics.technion.ac.il, E-mail: branchin@fis.uniroma3.it, E-mail: mdavis@berkeley.edu

2012-08-10

Using redshifts as a proxy for galaxy distances, estimates of the two-dimensional (2D) transverse peculiar velocities of distant galaxies could be obtained from future measurements of proper motions. We provide the mathematical framework for analyzing 2D transverse motions and show that they offer several advantages over traditional probes of large-scale motions. They are completely independent of any intrinsic relations between galaxy properties; hence, they are essentially free of selection biases. They are free from homogeneous and inhomogeneous Malmquist biases that typically plague distance indicator catalogs. They provide additional information to traditional probes that yield line-of-sight peculiar velocities only. Further, becausemore » of their 2D nature, fundamental questions regarding vorticity of large-scale flows can be addressed. Gaia, for example, is expected to provide proper motions of at least bright galaxies with high central surface brightness, making proper motions a likely contender for traditional probes based on current and future distance indicator measurements.« less

Subatomic-scale force vector mapping above a Ge(001) dimer using bimodal atomic force microscopy

NASA Astrophysics Data System (ADS)

Naitoh, Yoshitaka; Turanský, Robert; Brndiar, Ján; Li, Yan Jun; Štich, Ivan; Sugawara, Yasuhiro

2017-07-01

Probing physical quantities on the nanoscale that have directionality, such as magnetic moments, electric dipoles, or the force response of a surface, is essential for characterizing functionalized materials for nanotechnological device applications. Currently, such physical quantities are usually experimentally obtained as scalars. To investigate the physical properties of a surface on the nanoscale in depth, these properties must be measured as vectors. Here we demonstrate a three-force-component detection method, based on multi-frequency atomic force microscopy on the subatomic scale and apply it to a Ge(001)-c(4 × 2) surface. We probed the surface-normal and surface-parallel force components above the surface and their direction-dependent anisotropy and expressed them as a three-dimensional force vector distribution. Access to the atomic-scale force distribution on the surface will enable better understanding of nanoscale surface morphologies, chemical composition and reactions, probing nanostructures via atomic or molecular manipulation, and provide insights into the behaviour of nano-machines on substrates.

Large contact noise in graphene field-effect transistors

NASA Astrophysics Data System (ADS)

Karnatak, Paritosh; Sai, Phanindra; Goswami, Srijit; Ghatak, Subhamoy; Kaushal, Sanjeev; Ghosh, Arindam

Fluctuations in the electrical resistance at the interface of atomically thin materials and metals, or the contact noise, can adversely affect the device performance but remains largely unexplored. We have investigated contact noise in graphene field effect transistors of varying device geometry and contact configuration, with channel carrier mobility ranging from 5,000 to 80,000 cm2V-1s-1. A phenomenological model developed for contact noise due to current crowding for two dimensional conductors, shows a dominant contact contribution to the measured resistance noise in all graphene field effect transistors when measured in the two-probe or invasive four probe configurations, and surprisingly, also in nearly noninvasive four probe (Hall bar) configuration in the high mobility devices. We identify the fluctuating electrostatic environment of the metal-channel interface as the major source of contact noise, which could be generic to two dimensional material-based electronic devices. The work was financially supported by the Department of Science and Technology, India and Tokyo Electron Limited.

Molecular Imaging Probes for Positron Emission Tomography and Optical Imaging of Sentinel Lymph Node and Tumor

NASA Astrophysics Data System (ADS)

Qin, Zhengtao

Molecular imaging is visualizations and measurements of in vivo biological processes at the molecular or cellular level using specific imaging probes. As an emerging technology, biocompatible macromolecular or nanoparticle based targeted imaging probes have gained increasing popularities. Those complexes consist of a carrier, an imaging reporter, and a targeting ligand. The active targeting ability dramatically increases the specificity. And the multivalency effect may further reduce the dose while providing a decent signal. In this thesis, sentinel lymph node (SLN) mapping and cancer imaging are two research topics. The focus is to develop molecular imaging probes with high specificity and sensitivity, for Positron Emission Tomography (PET) and optical imaging. The objective of this thesis is to explore dextran radiopharmaceuticals and porous silicon nanoparticles based molecular imaging agents. Dextran polymers are excellent carriers to deliver imaging reporters or therapeutic agents due to its well established safety profile and oligosaccharide conjugation chemistry. There is also a wide selection of dextran polymers with different lengths. On the other hand, Silicon nanoparticles represent another class of biodegradable materials for imaging and drug delivery. The success in fluorescence lifetime imaging and enhancements of the immune activation potency was briefly discussed. Chapter 1 begins with an overview on current molecular imaging techniques and imaging probes. Chapter 2 presents a near-IR dye conjugated probe, IRDye 800CW-tilmanocept. Fluorophore density was optimized to generate the maximum brightness. It was labeled with 68Ga and 99mTc and in vivo SLN mapping was successfully performed in different animals, such as mice, rabbits, dogs and pigs. With 99mTc labeled IRDye 800CW-tilmanocept, chapter 3 introduces a two-day imaging protocol with a hand-held imager. Chapter 4 proposed a method to dual radiolabel the IRDye 800CW-tilmanocept with both 68Ga and 99mTc. Chapter 5 introduces a 68Ga metal chelating bioorthogonal tetrazine dextran probe for multistep imaging of a colon cancer. Chapter 6 presents the synthesis and in vivo evaluation of a Hepatocellular Carcinoma targeting PET probe 68Ga-Insulin-Dextran. Chapter 7 discusses a novel method to prepare silicon nanoparticles with great yield and size control. The last chapter 8 concludes all probes developed in this thesis and their clinical relevance.

Modelling of a laser-pumped light source for endoscopic surgery

NASA Astrophysics Data System (ADS)

Nadeau, Valerie J.; Elson, Daniel S.; Hanna, George B.; Neil, Mark A. A.

2008-09-01

A white light source, based on illumination of a yellow phosphor with a fibre-coupled blue-violet diode laser, has been designed and built for use in endoscopic surgery. This narrow light probe can be integrated into a standard laparoscope or inserted into the patient separately via a needle. We present a Monte Carlo model of light scattering and phosphorescence within the phosphor/silicone matrix at the probe tip, and measurements of the colour, intensity, and uniformity of the illumination. Images obtained under illumination with this light source are also presented, demonstrating the improvement in illumination quality over existing endoscopic light sources. This new approach to endoscopic lighting has the advantages of compact design, improved ergonomics, and more uniform illumination in comparison with current technologies.

Guidelines for transoesophageal echocardiographic probe cleaning and disinfection from the British Society of Echocardiography.

PubMed

Kanagala, P; Bradley, C; Hoffman, P; Steeds, R P

2011-10-01

The clinical utility of transoesophageal echocardiography (TOE) is well established. Being a semi-invasive procedure, however, the potential for transmission of infection between sequential patients exists. This has implications for the protection of both patients and medical staff. Guidelines for disinfection during gastrointestinal endoscopy (GIE) have been in place for many years.(1,2) Unfortunately, similar guidance is lacking with respect to TOE. Although traversing the same body cavities and sharing many similarities with upper GIE, there are fundamental structural and procedural differences with TOE which merit special consideration in establishing a decontamination protocol. This document provides recommendations for TOE probe decontamination based on the available evidence, expert opinion, and modification of the current British Society of Gastroenterology guidelines.

A noninterference blade vibration measurement system for gas turbine engines

NASA Astrophysics Data System (ADS)

Watkins, William B.; Chi, Ray M.

1987-06-01

A noninterfering blade vibration system has been demonstrated in tests of a gas turbine first stage fan. Conceptual design of the system, including its theory, design of case mounted probes, and data acquisition and signal processing hardware was done in a previous effort. The current effort involved instrumentation of an engine fan stage with strain gages; data acquisition using shaft-mounted reference and case-mounted optical probes; recording of data on a wideband tape recorder; and posttest processing using off-line analysis in a facility computer and a minicomputer-based readout system designed for near- real-time readout. Results are presented in terms of true blade vibration frequencies, time and frequency dependent vibration amplitudes and comparison of the optical noninterference results with strain gage readings.

A study of the high-precision displacement laser probe

NASA Astrophysics Data System (ADS)

Fan, Yuming; Zhang, Guoxiong

2006-06-01

On the basis of the measuring principle of the dynamic active optical confocal probe based on time difference measurement that has a reference path, a dynamic active optical confocal probe based on time difference measurement but has no reference path is developed. In this paper, the working principle of this optical confocal probe is dissertated. A large-scale integrated measuring system is designed to simplify the structure of the probe and to enhance the stability of the probe. Single-chip microcomputer system with a high-speed ADC is selected in the measurement and control system of the probe. At the end of the paper, experiments on the performance of the optical confocal probe based on time difference measurement with no reference path are carried out. Experiment results show that the probe has a measuring resolution of 0.05μm, a measuring range of 0.2mm and a linearity of 0.4μm.

New Eddy Current Probe for Thickness Gauging of Conductive Materials

NASA Technical Reports Server (NTRS)

Wincheski, Buzz; Fulton, Jim; Nath, Shridhar; Namkung, Min

1993-01-01

The accurate measure of material thickness is important for many non-destructive evaluation applications. Factors such as corrosion damage can jeopardize structural integrity through material thinning and process control considerations often mandate strict limits on material dimensions. Access to the material under test can be limited to a single side and large areas may need to be examined in a small time period. In an effort to enhance the effectiveness of material thickness measurements a flux focusing eddy current probe has been developed at NASA Langley Research Center. The probe provides an accurate measure of the thickness of conducting materials from a single sided measurement. It is straight forward to use and can be easily automated for production line testing. The probe also requires only minimal instrumentation and power so that extremely portable units can be manufactured at a low cost. This new eddy current probe has been used to accurately measure the thickness of aluminum alloy plates with a resolution of greater than 0.001 in. (25 microns). Simulated corrosion damage has also been detected on both single layer and multi-layer samples. The present work will explain the output voltage dependence of the device as a function of material thickness and present experimental results for thickness gauging and corrosion detection.

Single Color Multiplexed ddPCR Copy Number Measurements and Single Nucleotide Variant Genotyping.

PubMed

Wood-Bouwens, Christina M; Ji, Hanlee P

2018-01-01

Droplet digital PCR (ddPCR) allows for accurate quantification of genetic events such as copy number variation and single nucleotide variants. Probe-based assays represent the current "gold-standard" for detection and quantification of these genetic events. Here, we introduce a cost-effective single color ddPCR assay that allows for single genome resolution quantification of copy number and single nucleotide variation.

Basic aerodynamic research facility for comparative studies of flow diagnostic techniques

NASA Technical Reports Server (NTRS)

Jones, Gregory S.; Gartrell, Luther R.; Stainback, P. Calvin

1987-01-01

Current flow diagnostic research efforts are focusing on higher order flow field data bases, such as those generated by laser velocimetry (LV), hot-wire anemometry, and multi-hole pressure probes. Recent low-speed comparisons of results obtained with LV and hot wires have revealed strengths and weaknesses of each instrument. A seeding study will be initiated to determine particulate tracking ability.

UCB current detector experiment on Swedish auroral payloads. [ionospheric current and plasma flow measurements

NASA Technical Reports Server (NTRS)

Mozer, F.

1974-01-01

A split Langmuir probe has been developed to make in situ measurements of ionospheric current density and plasma bulk flow. The probe consists of two conducting elements that are separated by a thin insulator that shield each other over a 2 pi solid angle, and that are simultaneously swept from negative to positive with respect to the plasma. By measuring the current to each plate and the difference current between plates, information is obtained on the plasma's current density, bulk flow, electron temperature, and density. The instrument was successfully flown twice on sounding rockets into auroral events. Measurement data indicate that the total auroral current configuration is composed of several alternating east and west electrojets associated with several alternating up and down Birkeland currents.

Rectification of nanopores in aprotic solvents - transport properties of nanopores with surface dipoles

NASA Astrophysics Data System (ADS)

Plett, Timothy; Shi, Wenqing; Zeng, Yuhan; Mann, William; Vlassiouk, Ivan; Baker, Lane A.; Siwy, Zuzanna S.

2015-11-01

Nanopores have become a model system to understand transport properties at the nanoscale. We report experiments and modeling of ionic current in aprotic solvents with different dipole moments through conically shaped nanopores in a polycarbonate film and through glass nanopipettes. We focus on solutions of the salt LiClO4, which is of great importance in modeling lithium based batteries. Results presented suggest ion current rectification observed results from two effects: (i) adsorption of Li+ ions to the pore walls, and (ii) a finite dipole moment rendered by adsorbed solvent molecules. Properties of surfaces in various solvents were probed by means of scanning ion conductance microscopy, which confirmed existence of an effectively positive surface potential in aprotic solvents with high dipole moments.Nanopores have become a model system to understand transport properties at the nanoscale. We report experiments and modeling of ionic current in aprotic solvents with different dipole moments through conically shaped nanopores in a polycarbonate film and through glass nanopipettes. We focus on solutions of the salt LiClO4, which is of great importance in modeling lithium based batteries. Results presented suggest ion current rectification observed results from two effects: (i) adsorption of Li+ ions to the pore walls, and (ii) a finite dipole moment rendered by adsorbed solvent molecules. Properties of surfaces in various solvents were probed by means of scanning ion conductance microscopy, which confirmed existence of an effectively positive surface potential in aprotic solvents with high dipole moments. Electronic supplementary information (ESI) available. See DOI: 10.1039/c5nr06340j

Self-Nulling Lock-in Detection Electronics for Capacitance Probe Electrometer

NASA Technical Reports Server (NTRS)

Blaes, Brent R.; Schaefer, Rembrandt T.

2012-01-01

A multi-channel electrometer voltmeter that employs self-nulling lock-in detection electronics in conjunction with a mechanical resonator with noncontact voltage sensing electrodes has been developed for space-based measurement of an Internal Electrostatic Discharge Monitor (IESDM). The IESDM is new sensor technology targeted for integration into a Space Environmental Monitor (SEM) subsystem used for the characterization and monitoring of deep dielectric charging on spacecraft. Use of an AC-coupled lock-in amplifier with closed-loop sense-signal nulling via generation of an active guard-driving feedback voltage provides the resolution, accuracy, linearity and stability needed for long-term space-based measurement of the IESDM. This implementation relies on adjusting the feedback voltage to drive the sense current received from the resonator s variable-capacitance-probe voltage transducer to approximately zero, as limited by the signal-to-noise performance of the loop electronics. The magnitude of the sense current is proportional to the difference between the input voltage being measured and the feedback voltage, which matches the input voltage when the sense current is zero. High signal-to-noise-ratio (SNR) is achieved by synchronous detection of the sense signal using the correlated reference signal derived from the oscillator circuit that drives the mechanical resonator. The magnitude of the feedback voltage, while the loop is in a settled state with essentially zero sense current, is an accurate estimate of the input voltage being measured. This technique has many beneficial attributes including immunity to drift, high linearity, high SNR from synchronous detection of a single-frequency carrier selected to avoid potentially noisy 1/f low-frequency spectrum of the signal-chain electronics, and high accuracy provided through the benefits of a driven shield encasing the capacitance- probe transducer and guarded input triaxial lead-in. Measurements obtained from a 2- channel prototype electrometer have demonstrated good accuracy (|error| < 0.2 V) and high stability. Twenty-four-hour tests have been performed with virtually no drift. Additionally, 5,500 repeated one-second measurements of 100 V input were shown to be approximately normally distributed with a standard deviation of 140 mV.

Cross-section analysis of the Magnum-PSI plasma beam using a 2D multi-probe system

NASA Astrophysics Data System (ADS)

Costin, C.; Anita, V.; Ghiorghiu, F.; Popa, G.; De Temmerman, G.; van den Berg, M. A.; Scholten, J.; Brons, S.

2015-02-01

The linear plasma generator Magnum-PSI was designed for the study of plasma-surface interactions under relevant conditions of fusion devices. A key factor for such studies is the knowledge of a set of parameters that characterize the plasma interacting with the solid surface. This paper reports on the electrical diagnosis of the plasma beam in Magnum-PSI using a multi-probe system consisting of 64 probes arranged in a 2D square matrix. Cross-section distributions of floating potential and ion current intensity were registered for a hydrogen plasma beam under various discharge currents (80-175 A) and magnetic field strengths (0.47-1.41 T in the middle of the coils). Probe measurements revealed a high level of flexibility of plasma beam parameters with respect to the operating conditions.

Investigation of diocotron modes in toroidally trapped electron plasmas using non-destructive method

NASA Astrophysics Data System (ADS)

Lachhvani, Lavkesh; Pahari, Sambaran; Sengupta, Sudip; Yeole, Yogesh G.; Bajpai, Manu; Chattopadhyay, P. K.

2017-10-01

Experiments with trapped electron plasmas in a SMall Aspect Ratio Toroidal device (SMARTEX-C) have demonstrated a flute-like mode represented by oscillations on capacitive (wall) probes. Although analogous to diocotron mode observed in linear electron traps, the mode evolution in toroids can have interesting consequences due to the presence of in-homogeneous magnetic field. In SMARTEX-C, the probe signals are observed to undergo transition from small, near-sinusoidal oscillations to large amplitude, non-linear "double-peaked" oscillations. To interpret the wall probe signal and bring forth the dynamics, an expression for the induced current on the probe for an oscillating charge is derived, utilizing Green's Reciprocation Theorem. Equilibrium position, poloidal velocity of the charge cloud, and charge content of the cloud, required to compute the induced current, are estimated from the experiments. Signal through capacitive probes is thereby computed numerically for possible charge cloud trajectories. In order to correlate with experiments, starting with an intuitive guess of the trajectory, the model is evolved and tweaked to arrive at a signal consistent with experimentally observed probe signals. A possible vortex like dynamics is predicted, hitherto unexplored in toroidal geometries, for a limited set of experimental observations from SMARTEX-C. Though heuristic, a useful interpretation of capacitive probe data in terms of charge cloud dynamics is obtained.

Analysis of high field effects on the steady-state current-voltage response of semi-insulating 4H-SiC for photoconductive switch applications

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tiskumara, R.; Joshi, R. P., E-mail: ravi.joshi@ttu.edu; Mauch, D.

A model-based analysis of the steady-state, current-voltage response of semi-insulating 4H-SiC is carried out to probe the internal mechanisms, focusing on electric field driven effects. Relevant physical processes, such as multiple defects, repulsive potential barriers to electron trapping, band-to-trap impact ionization, and field-dependent detrapping, are comprehensively included. Results of our model match the available experimental data fairly well over orders of magnitude variation in the current density. A number of important parameters are also extracted in the process through comparisons with available data. Finally, based on our analysis, the possible presence of holes in the samples can be discounted upmore » to applied fields as high as ∼275 kV/cm.« less

Impact of Near-Earth Plasma Sheet Dynamics on the Ring Current Composition

NASA Astrophysics Data System (ADS)

Kistler, L. M.; Mouikis, C.; Menz, A.; Spence, H. E.; Mitchell, D. G.; Gkioulidou, M.; Lanzerotti, L. J.; Skoug, R. M.; Larsen, B.; Claudepierre, S. G.; Fennell, J. F.; Blake, J. B.

2014-12-01

How the dynamics in the near-earth plasma sheet affects the heavy ion content, and therefore the ion pressure, of the ring current in Earth's magnetosphere is an outstanding question. Substorms accelerate plasma in the near-earth region and drive outflow from the aurora, and both these processes can preferentially enhance the population of heavy ions in this region. These heavy ions are then driven into the inner magnetosphere during storms. Thus understanding how the composition of the ring current changes requires simultaneous observations in the near-earth plasma sheet and in the inner magnetosphere. We use data from the CODIF instrument on Cluster and HOPE, RBSPICE, and MagEIS instruments on the Van Allen Probes to study the acceleration and transport of ions from the plasma sheet into the ring current. During the main phase of a geomagnetic storm on Aug 4-6, 2013, the Cluster spacecraft were moving inbound in the midnight central plasma sheet, while the apogees of the two Van Allen Probes were located on the duskside. The Cluster spacecraft measure the composition and spectral changes in the plasma sheet, while the Van Allen Probes measure the ions that reach the inner magnetosphere. A strong increase in 1-40 keV O+ was observed at the Cluster location during the storm main phase, and the Van Allen Probes observed both H+ and O+ being driven deep into the inner magnetosphere. By comparing the variations in phase space density (PSD) vs. magnetic moment at the Cluster and the Van Allen Probes locations, we examine how the composition changes non-adiabatically in the near-earth plasma sheet, and how those changes are propagated into the inner magnetosphere, populating the hto ion ring current.

Design and evaluation of precise current integrator for scanning probe microscopy

NASA Astrophysics Data System (ADS)

Raczkowski, Kamil; Piasecki, Tomasz; Rudek, Maciej; Gotszalk, Teodor

2017-03-01

Several of the scanning probe microscopy (SPM) techniques, such as the scanning tunnelling microscopy (STM) or conductive atomic force microscopy (C-AFM), rely on precise measurements of current flowing between the investigated sample and the conductive nanoprobe. The parameters of current-to-voltage converter (CVC), which should detect current in the picompere range, are of utmost importance to those systems as they determine the microscopes’ measuring capabilities. That was the motivation for research on the precise current integrator (PCI), described in this paper, which could be used as the CVC in the C-AFM systems. The main design goal of the PCI was to provide a small and versatile device with the sub-picoampere level resolution with high dynamic range in the order of nanoamperes. The PCI was based on the integrating amplifier (Texas Instruments DDC112) paired with a STM32F4 microcontroller unit (MCU).The gain and bandwidth of the PCI might be easily changed by varying the integration time and the feedback capacitance. Depending on these parameters it was possible to obtain for example the 2.15 pA resolution at 688 nA range with 1 kHz bandwidth or 7.4 fA resolution at 0.98 nA range with 10 Hz bandwidth. The measurement of sinusoidal current with 28 fA amplitude was also presented. The PCI was integrated with the C-AFM system and used in the highly ordered pyrolytic graphite (HOPG) and graphene samples imaging.

Enzyme- and label-free electrochemical aptasensor for kanamycin detection based on double stir bar-assisted toehold-mediated strand displacement reaction for dual-signal amplification.

PubMed

Hong, Feng; Chen, Xixue; Cao, Yuting; Dong, Youren; Wu, Dazhen; Hu, Futao; Gan, Ning

2018-07-30

It is critically important to detect antibiotic residues for monitoring food safety. In this study, an enzyme- and label-free electrochemical aptasensor for antibiotics, with kanamycin (Kana) as a typical analyte, was developed based on a double stir bar-assisted toehold-mediated strand displacement reaction (dSB-TMSDR) for dual-signal amplification. First, we modified two gold electrodes (E-1 and E-2) with different DNA probes (S1/S2 hybrid probe in E-1 and DNA fuel strand S3 in E-2). In the presence of Kana, an S1/S2 probe can be disassembled from E-1 to form an S2/Kana complex in supernatant. The S2/Kana could react with S3 on E-2 to form S2/S3 hybrid and release Kana through TMSDR. After then, the target recycling was triggered. Subsequently, the formed S2/S3 hybrid can also trigger a hybridization chain reaction (HCR). Consequently, the dual-signal amplification strategy was established, which resulted in many long dsDNA chains on E-2. The chains can associate with methylene blue (MB) as redox probes to produce a current response for the quantification of Kana. The assay exhibited high sensitivity and specificity with a detection limit at 16 fM Kana due to the dual-signal amplification. The double stir bars system can both increase phase separation and prevent leakage of DNA fuel to reduce background interference. Moreover, it allows flexible sequence design of the TMSDR probes. The assay was successfully employed to detect Kana residues in food and showed potential application value in food safety detection. Copyright © 2018 Elsevier B.V. All rights reserved.

A micromachined membrane-based active probe for biomolecular mechanics measurement

NASA Astrophysics Data System (ADS)

Torun, H.; Sutanto, J.; Sarangapani, K. K.; Joseph, P.; Degertekin, F. L.; Zhu, C.

2007-04-01

A novel micromachined, membrane-based probe has been developed and fabricated as assays to enable parallel measurements. Each probe in the array can be individually actuated, and the membrane displacement can be measured with high resolution using an integrated diffraction-based optical interferometer. To illustrate its application in single-molecule mechanics experiments, this membrane probe was used to measure unbinding forces between L-selectin reconstituted in a polymer-cushioned lipid bilayer on the probe membrane and an antibody adsorbed on an atomic force microscope cantilever. Piconewton range forces between single pairs of interacting molecules were measured from the cantilever bending while using the membrane probe as an actuator. The integrated diffraction-based optical interferometer of the probe was demonstrated to have <10 fm Hz-1/2 noise floor for frequencies as low as 3 Hz with a differential readout scheme. With soft probe membranes, this low noise level would be suitable for direct force measurements without the need for a cantilever. Furthermore, the probe membranes were shown to have 0.5 µm actuation range with a flat response up to 100 kHz, enabling measurements at fast speeds.

Evaluation of the Self-Nulling Rotating Eddy Current Probe System

NASA Technical Reports Server (NTRS)

Hagemaier, Don; Rengel, Kent; Wincheski, Buzz; Namkung, Min

1999-01-01

In order to detect multi-site fatigue cracks located under flush-head rivets, automated eddy current equipment is required. To assure a reliable system, the eddy current probe must be centered easily over the installed rivets. To meet these requirements, the NDE Group at NASA LaRC developed the Self-Nulling Rotating Eddy Current Probe System (SNRECPS) which will be referred to as RPS in this document. The system was evaluated at the FAA, NDI Validation Center, in Albuquerque, New Mexico. The system was capable of detecting a 0.032 inch long crack with a 90/95% PoD. Further evaluations were conducted at Boeing in Long Beach, California. These evaluations included fatigue cracks and notches in a range from 0.025 to 0.100 inch long under flush-head aluminum rivets, and titanium or steel flush-head fasteners. The results of these tests are reported herein. Subsequently, the system was loaned to the USAF Structures Laboratory for the purpose of detecting and measuring short cracks under flush-head rivets in a variety of fatigue test specimens. The inspection task was to detect and plot crack growth from numbered fasteners in lettered rows. In January, 1998, the system was taken to Northwest Airlines Maintenance Base, in Atlanta, to inspect a DC-9, for multi-site cracks in three circumferential splices. The aircraft had 83,000 cycles. The inspection was conducted at 30 kHz from longeron 5 left to longeron 5 right. The system was calibrated using a 0,030 EDM first layer notch. The instrument gain was set to 19 mV from the notch. The reject level was set at 10 mV and the unflawed fasteners yielded a signal amplitude of 2 to 3 mV. Only one fastener location, out of about 2,500 tested, yielded a signal of 58 mV. The rivet was removed and visually evaluated. It appeared to be a slight gouge in the counter-sink zone. No fatigue cracks were detected. The same fastener locations were also inspected using the Boeing MAUS system at 60 kHz. No cracks were detected. Thus far, the rotating probe eddy current system has been found to be very user friendly and capable of detecting first layer cracks on the order of 0.030 inch long or longer.

Four-probe measurements with a three-probe scanning tunneling microscope

DOE Office of Scientific and Technical Information (OSTI.GOV)

Salomons, Mark; Martins, Bruno V. C.; Zikovsky, Janik

2014-04-15

We present an ultrahigh vacuum (UHV) three-probe scanning tunneling microscope in which each probe is capable of atomic resolution. A UHV JEOL scanning electron microscope aids in the placement of the probes on the sample. The machine also has a field ion microscope to clean, atomically image, and shape the probe tips. The machine uses bare conductive samples and tips with a homebuilt set of pliers for heating and loading. Automated feedback controlled tip-surface contacts allow for electrical stability and reproducibility while also greatly reducing tip and surface damage due to contact formation. The ability to register inter-tip position bymore » imaging of a single surface feature by multiple tips is demonstrated. Four-probe material characterization is achieved by deploying two tips as fixed current probes and the third tip as a movable voltage probe.« less

Biasing, Acquisition and Interpretation of a Dense Langmuir Probe Array in NSTX

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jaworski, M. A.; Kallman, J.; Kaita, R.

2010-09-22

A dense array of 99 Langmuir probes has been installed in the lower divertor region of the National Spherical Torus Experiments (NSTX). This array is instrumented with a system of elec- tronics that allows flexibility in the choice of probes to bias as well as the type of measurement (including standard swept, single probe, triple probe and operation as passive floating potential and scrape-off-layer (SOL) current monitors). The use of flush-mounted probes requires careful inter- pretation. The time dependent nature of the SOL makes swept-probe traces difficult to interpret. To overcome these challenges, the single- and triple-Langmuir probe signals aremore » used in comple- mentary fashion to determine the temperature and density at the probe location. A comparison to mid-plane measurements is made. Work is supported by DOE contracts DE-AC02-09CHI1466 and DE-PS02-07ER07-29.« less

High Aspect-Ratio Neural Probes using Conventional Blade Dicing

NASA Astrophysics Data System (ADS)

Goncalves, S. B.; Ribeiro, J. F.; Silva, A. F.; Correia, J. H.

2016-10-01

Exploring deep neural circuits has triggered the development of long penetrating neural probes. Moreover, driven by brain displacement, the long neural probes require also a high aspect-ratio shafts design. In this paper, a simple and reproducible method of manufacturing long-shafts neural probes using blade dicing technology is presented. Results shows shafts up to 8 mm long and 200 µm wide, features competitive to the current state-of-art, being its outline simply accomplished by a single blade dicing program. Therefore, conventional blade dicing presents itself as a viable option to manufacture long neural probes.

Contoured Surface Eddy Current Inspection System

DOEpatents

Batzinger, Thomas James; Fulton, James Paul; Rose, Curtis Wayne; Perocchi, Lee Cranford

2003-04-08

Eddy current inspection of a contoured surface of a workpiece is performed by forming a backing piece of flexible, resiliently yieldable material with a contoured exterior surface conforming in shape to the workpiece contoured surface. The backing piece is preferably cast in place so as to conform to the workpiece contoured surface. A flexible eddy current array probe is attached to the contoured exterior surface of the backing piece such that the probe faces the contoured surface of the workpiece to be inspected when the backing piece is disposed adjacent to the workpiece. The backing piece is then expanded volumetrically by inserting at least one shim into a slot in the backing piece to provide sufficient contact pressure between the probe and the workpiece contoured surface to enable the inspection of the workpiece contoured surface to be performed.

A measurement of perpendicular current density in an aurora

NASA Technical Reports Server (NTRS)

Bering, E. A.; Mozer, F. S.

1975-01-01

A Nike Tomahawk sounding rocket was launched into a 400-gamma auroral substorm from Esrange, Kiruna, Sweden. The rocket instrumentation included a split Langmuir-probe plasma-velocity detector and a double-probe electric-field detector. Above 140-km altitude, the electric field deduced from the ion-flow velocity measurement and the electric field measured by the double probe agree to an accuracy within the uncertainties of the two measurements. The difference between the two measurements at altitudes below 140 km provides an in situ measurement of current density and conductivity. Alternatively, if values for the conductivity are assumed, the neutral-wind velocity can be deduced. The height-integrated current was 0.11 A/m flowing at an azimuth angle of 276 deg. The neutral winds were strong, exhibited substantial altitude variation in the east-west component, and were predominantly southward.

All-optical optoacoustic microscopy system based on probe beam deflection technique

NASA Astrophysics Data System (ADS)

Maswadi, Saher M.; Tsyboulskic, Dmitri; Roth, Caleb C.; Glickman, Randolph D.; Beier, Hope T.; Oraevsky, Alexander A.; Ibey, Bennett L.

2016-03-01

It is difficult to achieve sub-micron resolution in backward mode OA microscopy using conventional piezoelectric detectors, because of wavefront distortions caused by components placed in the optical path, between the sample and the objective lens, that are required to separate the acoustic wave from the optical beam. As an alternate approach, an optoacoustic microscope (OAM) was constructed using the probe beam deflection technique (PBDT) to detect laserinduced acoustic signals. The all-optical OAM detects laser-generated pressure waves using a probe beam passing through a coupling medium, such as water, filling the space between the microscope objective lens and sample. The acoustic waves generated in the sample propagate through the coupling medium, causing transient changes in the refractive index that deflect the probe beam. These deflections are measured with a high-speed, balanced photodiode position detector. The deflection amplitude is directly proportional to the magnitude of the acoustic pressure wave, and provides the data required for image reconstruction. The sensitivity of the PBDT detector expressed as noise equivalent pressure was 12 Pa, comparable to that of existing high-performance ultrasound detectors. Because of the unimpeded working distance, a high numerical aperture objective lens, i.e. NA = 1, was employed in the OAM to achieve near diffraction-limited lateral resolution of 0.5 μm at 532nm. The all-optical OAM provides several benefits over current piezoelectric detector-based systems, such as increased lateral and axial resolution, higher sensitivity, robustness, and potentially more compatibility with multimodal instruments.

Probabilistic and Other Neural Nets in Multi-Hole Probe Calibration and Flow Angularity Pattern Recognition

NASA Technical Reports Server (NTRS)

Baskaran, Subbiah; Ramachandran, Narayanan; Noever, David

1998-01-01

The use of probabilistic (PNN) and multilayer feed forward (MLFNN) neural networks are investigated for calibration of multi-hole pressure probes and the prediction of associated flow angularity patterns in test flow fields. Both types of networks are studied in detail for their calibration and prediction characteristics. The current formalism can be applied to any multi-hole probe, however the test results for the most commonly used five-hole Cone and Prism probe types alone are reported in this article.

BEAM CONTROL PROBE

DOEpatents

Chesterman, A.W.

1959-03-17

A probe is described for intercepting a desired portion of a beam of charged particles and for indicating the spatial disposition of the beam. The disclosed probe assembly includes a pair of pivotally mounted vanes moveable into a single plane with adjacent edges joining and a calibrated mechanical arrangement for pivoting the vancs apart. When the probe is disposed in the path of a charged particle beam, the vanes may be adjusted according to the beam current received in each vane to ascertain the dimension of the beam.

Measurement of the target current by inductive probe during laser interaction on terawatt laser system PALS.

PubMed

Cikhardt, J; Krása, J; De Marco, M; Pfeifer, M; Velyhan, A; Krouský, E; Cikhardtová, B; Klír, D; Rezáč, K; Ullschmied, J; Skála, J; Kubeš, P; Kravárik, J

2014-10-01

Measurements of the return-current flowing through a solid target irradiated with the sub-nanosecond kJ-class Prague Asterix Laser System is reported. A new inductive target probe was developed which allows us measuring the target current derivative in a kA/ns range. The dependences of the target current on the laser pulse energy for cooper, graphite, and polyethylene targets are reported. The experiment shows that the target current is proportional to the deposited laser energy and is strongly affected by the shot-to-shot fluctuations. The corresponding maximum target charge exceeded a value of 10 μC. A return-current dependence of the electromagnetic pulse produced by the laser-target interaction is presented.

Competitive Reporter Monitored Amplification (CMA) - Quantification of Molecular Targets by Real Time Monitoring of Competitive Reporter Hybridization

PubMed Central

Ullrich, Thomas; Ermantraut, Eugen; Schulz, Torsten; Steinmetzer, Katrin

2012-01-01

Background State of the art molecular diagnostic tests are based on the sensitive detection and quantification of nucleic acids. However, currently established diagnostic tests are characterized by elaborate and expensive technical solutions hindering the development of simple, affordable and compact point-of-care molecular tests. Methodology and Principal Findings The described competitive reporter monitored amplification allows the simultaneous amplification and quantification of multiple nucleic acid targets by polymerase chain reaction. Target quantification is accomplished by real-time detection of amplified nucleic acids utilizing a capture probe array and specific reporter probes. The reporter probes are fluorescently labeled oligonucleotides that are complementary to the respective capture probes on the array and to the respective sites of the target nucleic acids in solution. Capture probes and amplified target compete for reporter probes. Increasing amplicon concentration leads to decreased fluorescence signal at the respective capture probe position on the array which is measured after each cycle of amplification. In order to observe reporter probe hybridization in real-time without any additional washing steps, we have developed a mechanical fluorescence background displacement technique. Conclusions and Significance The system presented in this paper enables simultaneous detection and quantification of multiple targets. Moreover, the presented fluorescence background displacement technique provides a generic solution for real time monitoring of binding events of fluorescently labelled ligands to surface immobilized probes. With the model assay for the detection of human immunodeficiency virus type 1 and 2 (HIV 1/2), we have been able to observe the amplification kinetics of five targets simultaneously and accommodate two additional hybridization controls with a simple instrument set-up. The ability to accommodate multiple controls and targets into a single assay and to perform the assay on simple and robust instrumentation is a prerequisite for the development of novel molecular point of care tests. PMID:22539973

Fluorescent-protein-based probes: general principles and practices.

PubMed

Ai, Hui-Wang

2015-01-01

An important application of fluorescent proteins is to derive genetically encoded fluorescent probes that can actively respond to cellular dynamics such as pH change, redox signaling, calcium oscillation, enzyme activities, and membrane potential. Despite the large diverse group of fluorescent-protein-based probes, a few basic principles have been established and are shared by most of these probes. In this article, the focus is on these general principles and strategies that guide the development of fluorescent-protein-based probes. A few examples are provided in each category to illustrate the corresponding principles. Since these principles are quite straightforward, others may adapt them to create fluorescent probes for their own interest. Hopefully, the development of the ever-growing family of fluorescent-protein-based probes will no longer be limited to a small number of laboratories specialized in senor development, leading to the situation that biological studies will be bettered assisted by genetically encoded sensors.

Plume Characterization of Busek 600W Hall Thruster

DTIC Science & Technology

2012-03-09

probe was used to examine the thruster plume current density while the ion species fractions were determined by the ExB probe. The inverted pendulum ...25 A. Inverted Pendulum ...Diagnostic Equipment .....................................................................................45 A. Inverted Pendulum

21 CFR 884.4160 - Unipolar endoscopic coagulator-cutter and accessories.

Code of Federal Regulations, 2010 CFR

2010-04-01

... temperatures by directing a high frequency electrical current through the tissue between an energized probe and... generator, probes and electrical cables, and a patient grounding plate. This generic type of device does not...

21 CFR 884.4160 - Unipolar endoscopic coagulator-cutter and accessories.

Code of Federal Regulations, 2011 CFR

2011-04-01

... temperatures by directing a high frequency electrical current through the tissue between an energized probe and... generator, probes and electrical cables, and a patient grounding plate. This generic type of device does not...

21 CFR 884.4160 - Unipolar endoscopic coagulator-cutter and accessories.

Code of Federal Regulations, 2012 CFR

2012-04-01

... temperatures by directing a high frequency electrical current through the tissue between an energized probe and... generator, probes and electrical cables, and a patient grounding plate. This generic type of device does not...

21 CFR 884.4160 - Unipolar endoscopic coagulator-cutter and accessories.

Code of Federal Regulations, 2014 CFR

2014-04-01

... temperatures by directing a high frequency electrical current through the tissue between an energized probe and... generator, probes and electrical cables, and a patient grounding plate. This generic type of device does not...

21 CFR 884.4160 - Unipolar endoscopic coagulator-cutter and accessories.

Code of Federal Regulations, 2013 CFR

2013-04-01

... temperatures by directing a high frequency electrical current through the tissue between an energized probe and... generator, probes and electrical cables, and a patient grounding plate. This generic type of device does not...

Two-photon absorbing porphyrins for oxygen microscopy (Conference Presentation)

NASA Astrophysics Data System (ADS)

Esipova, Tatiana V.; Vinogradov, Sergei A.

2016-03-01

The ability to quantify oxygen in vivo in 3D with high spatial and temporal resolution is invaluable for many areas of the biomedical science, including ophthalmology, neuroscience, cancer and stem biology. An optical method based on oxygen-dependent quenching of phosphorescence is being developed, that allows quantitative minimally invasive real-time imaging of partial pressure of oxygen (pO2) in tissue. In the past, dendritically protected phosphorescent oxygen probes with controllable quenching parameters and defined bio-distributions have been developed. More recently our probe strategy has extended to encompass two-photon excitable oxygen probes, which brought about first demonstrations of two-photon phosphorescence lifetime microscopy (2PLM) of oxygen in vivo, providing new valuable information for neuroscience and stem cell biology. However, current two-photon oxygen probes suffer from a number of limitations, such as low brightness and high cost of synthesis, which dramatically reduce imaging performance and limit usability of the method. Here we present an approach to new bright phosphorescent chromophores with internally enhanced two-photon absorption cross-sections, which pave a way to novel proves for 2PLM. In addition to substantial increase in performance, the new probes can be synthesized by much more efficient methods, thereby greatly reducing the cost of the synthesis and making the technique accessible to a broader range of researchers across different fields.

Integration of silicon-based neural probes and micro-drive arrays for chronic recording of large populations of neurons in behaving animals

NASA Astrophysics Data System (ADS)

Michon, Frédéric; Aarts, Arno; Holzhammer, Tobias; Ruther, Patrick; Borghs, Gustaaf; McNaughton, Bruce; Kloosterman, Fabian

2016-08-01

Objective. Understanding how neuronal assemblies underlie cognitive function is a fundamental question in system neuroscience. It poses the technical challenge to monitor the activity of populations of neurons, potentially widely separated, in relation to behaviour. In this paper, we present a new system which aims at simultaneously recording from a large population of neurons from multiple separated brain regions in freely behaving animals. Approach. The concept of the new device is to combine the benefits of two existing electrophysiological techniques, i.e. the flexibility and modularity of micro-drive arrays and the high sampling ability of electrode-dense silicon probes. Main results. Newly engineered long bendable silicon probes were integrated into a micro-drive array. The resulting device can carry up to 16 independently movable silicon probes, each carrying 16 recording sites. Populations of neurons were recorded simultaneously in multiple cortical and/or hippocampal sites in two freely behaving implanted rats. Significance. Current approaches to monitor neuronal activity either allow to flexibly record from multiple widely separated brain regions (micro-drive arrays) but with a limited sampling density or to provide denser sampling at the expense of a flexible placement in multiple brain regions (neural probes). By combining these two approaches and their benefits, we present an alternative solution for flexible and simultaneous recordings from widely distributed populations of neurons in freely behaving rats.

Report on the Current Inventory of the Toolbox for Plant Cell Wall Analysis: Proteinaceous and Small Molecular Probes

PubMed Central

Rydahl, Maja G.; Hansen, Aleksander R.; Kračun, Stjepan K.; Mravec, Jozef

2018-01-01

Plant cell walls are highly complex structures composed of diverse classes of polysaccharides, proteoglycans, and polyphenolics, which have numerous roles throughout the life of a plant. Significant research efforts aim to understand the biology of this cellular organelle and to facilitate cell-wall-based industrial applications. To accomplish this, researchers need to be provided with a variety of sensitive and specific detection methods for separate cell wall components, and their various molecular characteristics in vitro as well as in situ. Cell wall component-directed molecular detection probes (in short: cell wall probes, CWPs) are an essential asset to the plant glycobiology toolbox. To date, a relatively large set of CWPs has been produced—mainly consisting of monoclonal antibodies, carbohydrate-binding modules, synthetic antibodies produced by phage display, and small molecular probes. In this review, we summarize the state-of-the-art knowledge about these CWPs; their classification and their advantages and disadvantages in different applications. In particular, we elaborate on the recent advances in non-conventional approaches to the generation of novel CWPs, and identify the remaining gaps in terms of target recognition. This report also highlights the addition of new “compartments” to the probing toolbox, which is filled with novel chemical biology tools, such as metabolic labeling reagents and oligosaccharide conjugates. In the end, we also forecast future developments in this dynamic field. PMID:29774041

Experimental Studies of Intent Information on Cockpit Traffic Displays

NASA Technical Reports Server (NTRS)

Barhydt, Richard; Hansman, R. John, Jr.

1997-01-01

Intent information provides knowledge of another aircraft's current and future trajectory states. Prototype traffic displays were designed for four different levels of intent: No Intent, Rate, Commanded State, and Flight Management System (FMS)-Path. The TCAS Display was used as a baseline and represents the No Intent Level. The Rate, Commanded State, and FMS-Path Displays show increasing levels of intent information using TCAS-like symbology in addition to incorporating a conflict probe and profile view display. An experiment was run on the MIT Part Task Flight Simulator in which eight airline pilots flew five traffic scenarios with each of the four displays. Results show that pilots had fewer separation violations and maneuvered earlier with the three intent displays. Separation violations were reduced when pilots maneuvered earlier. A second experiment was run to compare performance between displaying intent information directly and incorporating it into a conflict probe. A different set of eight airline pilots flew four traffic scenarios with the TCAS and Commanded State Displays with and without the conflict probe. Conflict probes with two minute and long range look-ahead times were tested. Displaying conflict bands or showing intent information directly both led to fewer separation violations and earlier avoidance maneuvers than the base TCAS Display. Performance was similar between the two minute and long range look-ahead conflict probes. Pilots preferred all intent displays over the TCAS Display.

Enhanced Eddy-Current Detection Of Weld Flaws

NASA Technical Reports Server (NTRS)

Van Wyk, Lisa M.; Willenberg, James D.

1992-01-01

Mixing of impedances measured at different frequencies reduces noise and helps reveal flaws. In new method, one excites eddy-current probe simultaneously at two different frequencies; usually, one of which integral multiple of other. Resistive and reactive components of impedance of eddy-current probe measured at two frequencies, mixed in computer, and displayed in real time on video terminal of computer. Mixing of measurements obtained at two different frequencies often "cleans up" displayed signal in situations in which band-pass filtering alone cannot: mixing removes most noise, and displayed signal resolves flaws well.

Dose verification of eye plaque brachytherapy using spectroscopic dosimetry.

PubMed

Jarema, T; Cutajar, D; Weaver, M; Petasecca, M; Lerch, M; Kejda, A; Rosenfeld, A

2016-09-01

Eye plaque brachytherapy has been developed and refined for the last 80 years, demonstrating effective results in the treatment of ocular malignancies. Current dosimetry techniques for eye plaque brachytherapy (such as TLD- and film-based techniques) are time consuming and cannot be used prior to treatment in a sterile environment. The measurement of the expected dose distribution within the eye, prior to insertion within the clinical setting, would be advantageous, as any errors in source loading will lead to an erroneous dose distribution and inferior treatment outcomes. This study investigated the use of spectroscopic dosimetry techniques for real-time quality assurance of I-125 based eye plaques, immediately prior to insertion. A silicon detector based probe, operating in spectroscopy mode was constructed, containing a small (1 mm(3)) silicon detector, mounted within a ceramic holder, all encapsulated within a rubber sheath to prevent water infiltration of the electronics. Preliminary tests of the prototype demonstrated that the depth dose distribution through the central axis of an I-125 based eye plaque may be determined from AAPM Task Group 43 recommendations to a deviation of 6 % at 3 mm depth, 7 % at 5 mm depth, 1 % at 10 mm depth and 13 % at 20 mm depth, with the deviations attributed to the construction of the probe. A new probe design aims to reduce these discrepancies, however the concept of spectroscopic dosimetry shows great promise for use in eye plaque quality assurance in the clinical setting.

Eddy-Current Detection of Cracks in Tubes

NASA Technical Reports Server (NTRS)

Parent, R.; Kettering, D.

1987-01-01

Nondestructive device tests narrow, sharply-bent metal tubes. Eddycurrent probe detects incipient cracks inside small metal tubes. Tube-centering device consisting of pair of opposed bars ensures tube centered on eddy-current coil. Probe moves along length of bent tube to inspect repeatably for cracks. Compatible with tubes of different cross sections, oval, flattened, square, rectangular,or irregular. Adapts for inspecting formed tubes in petrochemical, automotive, nuclear, and medical equipment.

Mode control using two electrodes on HBT-EP

NASA Astrophysics Data System (ADS)

Stewart, I. G.; Brooks, J. W.; Levesque, J. P.; Mauel, M. E.; Navratil, G. A.

2017-10-01

Understanding the effects of plasma rotation on magnetohydrodynamic (MHD) modes and tokamak plasma stability is important for performance enhancement of current magnetic confinement experiments and to future fusion devices such as ITER. In order to control plasma rotation, two molybdenum electrodes have been installed on HBT-EP toroidally separated by 144 degrees. This allows independent biasing of the two probes both spatially and temporally. When the bias probes are inserted into the edge of the plasma and a voltage is applied, the probes drive radial currents and produce plasma flow from the torque induced by the currents. If the bias probe voltage is sufficiently positive, the MHD mode rotation transitions into a state with a rapid mode rotation frequency (in excess of 25 kHz) in the direction opposite to mode rotation without bias. The transition into this reversed rotation state occurs when the torque exceeds a threshold, which can depend upon the phase of an applied n = 1 error field. We present recent studies of the two-electrode system on mode rotation, mode stability, and the toroidal symmetry of the radial current through the scrape-off-layer (SOL) during MHD activity and applied magnetic perturbations. Supported by U.S. DOE Grant DE-FG02-86ER53222.

Synthesis and characterization of time-resolved fluorescence probes for evaluation of competitive binding to melanocortin receptors.

PubMed

Alleti, Ramesh; Vagner, Josef; Dehigaspitiya, Dilani Chathurika; Moberg, Valerie E; Elshan, N G R D; Tafreshi, Narges K; Brabez, Nabila; Weber, Craig S; Lynch, Ronald M; Hruby, Victor J; Gillies, Robert J; Morse, David L; Mash, Eugene A

2013-09-01

Probes for use in time-resolved fluorescence competitive binding assays at melanocortin receptors based on the parental ligands MSH(4), MSH(7), and NDP-α-MSH were prepared by solid phase synthesis methods, purified, and characterized. The saturation binding of these probes was studied using HEK-293 cells engineered to overexpress the human melanocortin 4 receptor (hMC4R) as well as the human cholecystokinin 2 receptor (hCCK2R). The ratios of non-specific binding to total binding approached unity at high concentrations for each probe. At low probe concentrations, receptor-mediated binding and uptake was discernable, and so probe concentrations were kept as low as possible in determining Kd values. The Eu-DTPA-PEGO-MSH(4) probe exhibited low specific binding relative to non-specific binding, even at low nanomolar concentrations, and was deemed unsuitable for use in competition binding assays. The Eu-DTPA-PEGO probes based on MSH(7) and NDP-α-MSH exhibited Kd values of 27±3.9nM and 4.2±0.48nM, respectively, for binding with hMC4R. These probes were employed in competitive binding assays to characterize the interactions of hMC4R with monovalent and divalent MSH(4), MSH(7), and NDP-α-MSH constructs derived from squalene. Results from assays with both probes reflected only statistical enhancements, suggesting improper ligand spacing on the squalene scaffold for the divalent constructs. The Ki values from competitive binding assays that employed the MSH(7)-based probe were generally lower than the Ki values obtained when the probe based on NDP-α-MSH was employed, which is consistent with the greater potency of the latter probe. The probe based on MSH(7) was also competed with monovalent, divalent, and trivalent MSH(4) constructs that previously demonstrated multivalent binding in competitive binding assays against a variant of the probe based on NDP-α-MSH. Results from these assays confirm multivalent binding, but suggest a more modest increase in avidity for these MSH(4) constructs than was previously reported. Copyright © 2013 Elsevier Ltd. All rights reserved.