Damage Progression in Bolted Composites

NASA Technical Reports Server (NTRS)

Minnetyan, Levon; Chamis, Christos; Gotsis, Pascal K.

1998-01-01

Structural durability,damage tolerance,and progressive fracture characteristics of bolted graphite/epoxy composite laminates are evaluated via computational simulation. Constituent material properties and stress and strain limits are scaled up to the structure level to evaluate the overall damage and fracture propagation for bolted composites. Single and double bolted composite specimens with various widths and bolt spacings are evaluated. The effect of bolt spacing is investigated with regard to the structural durability of a bolted joint. Damage initiation, growth, accumulation, and propagation to fracture are included in the simulations. Results show the damage progression sequence and structural fracture resistance during different degradation stages. A procedure is outlined for the use of computational simulation data in the assessment of damage tolerance, determination of sensitive parameters affecting fracture, and interpretation of experimental results with insight for design decisions.

Failure models for textile composites

NASA Technical Reports Server (NTRS)

Cox, Brian

1995-01-01

The goals of this investigation were to: (1) identify mechanisms of failure and determine how the architecture of reinforcing fibers in 3D woven composites controlled stiffness, strength, strain to failure, work of fracture, notch sensitivity, and fatigue life; and (2) to model composite stiffness, strength, and fatigue life. A total of 11 different angle and orthogonal interlock woven composites were examined. Composite properties depended on the weave architecture, the tow size, and the spatial distributions and strength of geometrical flaws. Simple models were developed for elastic properties, strength, and fatigue life. A more complicated stochastic model, called the 'Binary Model,' was developed for damage tolerance and ultimate failure. These 3D woven composites possessed an extraordinary combination of strength, damage tolerance, and notch insensitivity.

High damage tolerance of electrochemically lithiated silicon

PubMed Central

Wang, Xueju; Fan, Feifei; Wang, Jiangwei; Wang, Haoran; Tao, Siyu; Yang, Avery; Liu, Yang; Beng Chew, Huck; Mao, Scott X.; Zhu, Ting; Xia, Shuman

2015-01-01

Mechanical degradation and resultant capacity fade in high-capacity electrode materials critically hinder their use in high-performance rechargeable batteries. Despite tremendous efforts devoted to the study of the electro–chemo–mechanical behaviours of high-capacity electrode materials, their fracture properties and mechanisms remain largely unknown. Here we report a nanomechanical study on the damage tolerance of electrochemically lithiated silicon. Our in situ transmission electron microscopy experiments reveal a striking contrast of brittle fracture in pristine silicon versus ductile tensile deformation in fully lithiated silicon. Quantitative fracture toughness measurements by nanoindentation show a rapid brittle-to-ductile transition of fracture as the lithium-to-silicon molar ratio is increased to above 1.5. Molecular dynamics simulations elucidate the mechanistic underpinnings of the brittle-to-ductile transition governed by atomic bonding and lithiation-induced toughening. Our results reveal the high damage tolerance in amorphous lithium-rich silicon alloys and have important implications for the development of durable rechargeable batteries. PMID:26400671

High damage tolerance of electrochemically lithiated silicon

NASA Astrophysics Data System (ADS)

Wang, Xueju; Fan, Feifei; Wang, Jiangwei; Wang, Haoran; Tao, Siyu; Yang, Avery; Liu, Yang; Beng Chew, Huck; Mao, Scott X.; Zhu, Ting; Xia, Shuman

2015-09-01

Mechanical degradation and resultant capacity fade in high-capacity electrode materials critically hinder their use in high-performance rechargeable batteries. Despite tremendous efforts devoted to the study of the electro-chemo-mechanical behaviours of high-capacity electrode materials, their fracture properties and mechanisms remain largely unknown. Here we report a nanomechanical study on the damage tolerance of electrochemically lithiated silicon. Our in situ transmission electron microscopy experiments reveal a striking contrast of brittle fracture in pristine silicon versus ductile tensile deformation in fully lithiated silicon. Quantitative fracture toughness measurements by nanoindentation show a rapid brittle-to-ductile transition of fracture as the lithium-to-silicon molar ratio is increased to above 1.5. Molecular dynamics simulations elucidate the mechanistic underpinnings of the brittle-to-ductile transition governed by atomic bonding and lithiation-induced toughening. Our results reveal the high damage tolerance in amorphous lithium-rich silicon alloys and have important implications for the development of durable rechargeable batteries.

Progressive Fracture of Fiber Composite Thin Shell Structures Under Internal Pressure and Axial Loads

NASA Technical Reports Server (NTRS)

Gotsis, Pascal K.; Chamis, Christos C.; Minnetyan, Levon

1996-01-01

Graphite/epoxy composite thin shell structures were simulated to investigate damage and fracture progression due to internal pressure and axial loading. Defective and defect-free structures (thin cylinders) were examined. The three different laminates examined had fiber orientations of (90/0/+/-0)(sub s), where 0 is 45, 60, and 75 deg. CODSTRAN, an integrated computer code that scales up constituent level properties to the structural level and accounts for all possible failure modes, was used to simulate composite degradation under loading. Damage initiation, growth, accumulation, and propagation to fracture were included in the simulation. Burst pressures for defective and defect-free shells were compared to evaluate damage tolerance. The results showed that damage initiation began with matrix failure whereas damage and/or fracture progression occurred as a result of additional matrix failure and fiber fracture. In both thin cylinder cases examined (defective and defect-free), the optimum layup configuration was (90/0/+/-60)(sub s) because it had the best damage tolerance with respect to the burst pressure.

Recent progress in NASA Langley textile reinforced composites program

NASA Technical Reports Server (NTRS)

Dexter, H. Benson; Harris, Charles E.; Johnston, Norman J.

1992-01-01

The NASA LaRC is conducting and sponsoring research to explore the benefits of textile reinforced composites for civil transport aircraft primary structures. The objective of this program is to develop and demonstrate the potential of affordable textile reinforced composite materials to meet design properties and damage tolerance requirements of advanced aircraft structural concepts. In addition to in-house research, the program was recently expanded to include major participation by the aircraft industry and aerospace textile companies. The major program elements include development of textile preforms, processing science, mechanics of materials, experimental characterization of materials, and development and evaluation of textile reinforced composite structural elements and subcomponents. The NASA Langley in-house focus is as follows: development of a science-based understanding of resin transfer molding (RTM), development of powder-coated towpreg processes, analysis methodology, and development of a performance database on textile reinforced composites. The focus of the textile industry participation is on development of multidirectional, damage-tolerant preforms, and the aircraft industry participation is in the areas of design, fabrication and testing of textile reinforced composite structural elements and subcomponents. Textile processes such as 3D weaving, 2D and 3D braiding, and knitting/stitching are being compared with conventional laminated tape processes for improved damage tolerance. Through-the-thickness reinforcements offer significant damage tolerance improvements. However, these gains must be weighed against potential loss in in-plane properties such as strength and stiffness. Analytical trade studies are underway to establish design guidelines for the application of textile material forms to meet specific loading requirements. Fabrication and testing of large structural components are required to establish the full potential of textile reinforced composite materials.

Status Report on Irradiation Capsules Containing Welded FeCrAl Specimens for Radiation Tolerance Evaluation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Field, Kevin G.; Howard, Richard H.

2016-02-26

This status report provides the background and current status of a series of irradiation capsules, or “rabbits”, that were designed and built to test the contributions of microstructure, composition, damage dose, and irradiation temperature on the radiation tolerance of candidate FeCrAl alloys being developed to have enhanced weldability and radiation tolerance. These rabbits will also test the validity of using an ultra-miniature tensile specimen to assess the mechanical properties of irradiated FeCrAl base metal and weldments. All rabbits are to be irradiated in the High Flux Isotope Reactor (HFIR) at Oak Ridge National Laboratory (ORNL) to damage doses up tomore » ≥15 dpa at temperatures between 200-550°C.« less

High damage tolerance of electrochemically lithiated silicon

DOE PAGES

Wang, Xueju; Fan, Feifei; Wang, Jiangwei; ...

2015-09-24

Mechanical degradation and resultant capacity fade in high-capacity electrode materials critically hinder their use in high-performance rechargeable batteries. Despite tremendous efforts devoted to the study of the electro–chemo–mechanical behaviours of high-capacity electrode materials, their fracture properties and mechanisms remain largely unknown. In this paper, we report a nanomechanical study on the damage tolerance of electrochemically lithiated silicon. Our in situ transmission electron microscopy experiments reveal a striking contrast of brittle fracture in pristine silicon versus ductile tensile deformation in fully lithiated silicon. Quantitative fracture toughness measurements by nanoindentation show a rapid brittle-to-ductile transition of fracture as the lithium-to-silicon molar ratiomore » is increased to above 1.5. Molecular dynamics simulations elucidate the mechanistic underpinnings of the brittle-to-ductile transition governed by atomic bonding and lithiation-induced toughening. Finally, our results reveal the high damage tolerance in amorphous lithium-rich silicon alloys and have important implications for the development of durable rechargeable batteries.« less

Impact response of composite materials

NASA Technical Reports Server (NTRS)

Tiwari, S. N.; Srinivasan, K.

1991-01-01

Composite materials composed of carbon fibers and resin matrices offer great promise in reducing the weight of aerospace structures. However they remain extremely vulnerable to out of plane impact loads, which lead to severe losses in strength and stiffness. The results of an experimental program, undertaken to investigate the low velocity impact damage tolerance of composite materials is presented. The objectives were to identify key neat resin/composite properties that lead to enhancement of composite impact damage tolerance and to find a small scale test that predicts compression after impact properties of panels. Five materials were selected for evaluation. These systems represented different classes of material behavior such as brittle epoxy, modified epoxies, and amorphous and semicrystalling thermoplastics. The influence of fiber properties on the impact performance was also studied in one material, i.e., in polyether ether ketone (PEEK). Several 24 and 48 ply quasi-isotropic and 24 ply orthotropic laminates were examined using an instrumented drop weight impactor. Correlations with post impact compression behavior were made.

Damage Tolerance of Large Shell Structures

NASA Technical Reports Server (NTRS)

Minnetyan, L.; Chamis, C. C.

1999-01-01

Progressive damage and fracture of large shell structures is investigated. A computer model is used for the assessment of structural response, progressive fracture resistance, and defect/damage tolerance characteristics. Critical locations of a stiffened conical shell segment are identified. Defective and defect-free computer models are simulated to evaluate structural damage/defect tolerance. Safe pressurization levels are assessed for the retention of structural integrity at the presence of damage/ defects. Damage initiation, growth, accumulation, and propagation to fracture are included in the simulations. Damage propagation and burst pressures for defective and defect-free shells are compared to evaluate damage tolerance. Design implications with regard to defect and damage tolerance of a large steel pressure vessel are examined.

Investigation of progressive failure robustness and alternate load paths for damage tolerant structures

NASA Astrophysics Data System (ADS)

Marhadi, Kun Saptohartyadi

Structural optimization for damage tolerance under various unforeseen damage scenarios is computationally challenging. It couples non-linear progressive failure analysis with sampling-based stochastic analysis of random damage. The goal of this research was to understand the relationship between alternate load paths available in a structure and its damage tolerance, and to use this information to develop computationally efficient methods for designing damage tolerant structures. Progressive failure of a redundant truss structure subjected to small random variability was investigated to identify features that correlate with robustness and predictability of the structure's progressive failure. The identified features were used to develop numerical surrogate measures that permit computationally efficient deterministic optimization to achieve robustness and predictability of progressive failure. Analysis of damage tolerance on designs with robust progressive failure indicated that robustness and predictability of progressive failure do not guarantee damage tolerance. Damage tolerance requires a structure to redistribute its load to alternate load paths. In order to investigate the load distribution characteristics that lead to damage tolerance in structures, designs with varying degrees of damage tolerance were generated using brute force stochastic optimization. A method based on principal component analysis was used to describe load distributions (alternate load paths) in the structures. Results indicate that a structure that can develop alternate paths is not necessarily damage tolerant. The alternate load paths must have a required minimum load capability. Robustness analysis of damage tolerant optimum designs indicates that designs are tailored to specified damage. A design Optimized under one damage specification can be sensitive to other damages not considered. Effectiveness of existing load path definitions and characterizations were investigated for continuum structures. A load path definition using a relative compliance change measure (U* field) was demonstrated to be the most useful measure of load path. This measure provides quantitative information on load path trajectories and qualitative information on the effectiveness of the load path. The use of the U* description of load paths in optimizing structures for effective load paths was investigated.

Radiation Tolerant Interfaces: Influence of Local Stoichiometry at the Misfit Dislocation on Radiation Damage Resistance of Metal/Oxide Interfaces

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shutthanandan, Vaithiyalingam; Choudhury, Samrat; Manandhar, Sandeep

The interaction of radiation with materials controls the performance, reliability, and safety of many structures in nuclear power systems. Revolutionary improvements in radiation damage resistance may be attainable if methods can be found to manipulate interface properties to give optimal interface stability and point defect recombination capability. To understand how variations in interface properties such as misfit dislocation density and local chemistry affect radiation-induced defect absorption and recombination, a model system of metallic Cr xV 1-x (0 ≤ x ≤ 1) epitaxial films deposited on MgO(001) single crystal substrates has been explored in this paper. By controlling film composition, themore » lattice mismatch between the film and MgO is adjusted to vary the misfit dislocation density at the metal/oxide interface. The stability of these interfaces under various irradiation conditions is studied experimentally and theoretically. The results indicate that, unlike at metal/metal interfaces, the misfit dislocation density does not dominate radiation damage tolerance at metal/oxide interfaces. Rather, the stoichiometry and the location of the misfit dislocation extra half-plane (in the metal or the oxide) drive radiation-induced defect behavior. Finally, together, these results demonstrate the sensitivity of defect recombination to interfacial chemistry and provide new avenues for engineering radiation-tolerant nanomaterials for next-generation nuclear power plants.« less

Radiation Tolerant Interfaces: Influence of Local Stoichiometry at the Misfit Dislocation on Radiation Damage Resistance of Metal/Oxide Interfaces

DOE PAGES

Shutthanandan, Vaithiyalingam; Choudhury, Samrat; Manandhar, Sandeep; ...

2017-04-24

The interaction of radiation with materials controls the performance, reliability, and safety of many structures in nuclear power systems. Revolutionary improvements in radiation damage resistance may be attainable if methods can be found to manipulate interface properties to give optimal interface stability and point defect recombination capability. To understand how variations in interface properties such as misfit dislocation density and local chemistry affect radiation-induced defect absorption and recombination, a model system of metallic Cr xV 1-x (0 ≤ x ≤ 1) epitaxial films deposited on MgO(001) single crystal substrates has been explored in this paper. By controlling film composition, themore » lattice mismatch between the film and MgO is adjusted to vary the misfit dislocation density at the metal/oxide interface. The stability of these interfaces under various irradiation conditions is studied experimentally and theoretically. The results indicate that, unlike at metal/metal interfaces, the misfit dislocation density does not dominate radiation damage tolerance at metal/oxide interfaces. Rather, the stoichiometry and the location of the misfit dislocation extra half-plane (in the metal or the oxide) drive radiation-induced defect behavior. Finally, together, these results demonstrate the sensitivity of defect recombination to interfacial chemistry and provide new avenues for engineering radiation-tolerant nanomaterials for next-generation nuclear power plants.« less

Nanotechnology

NASA Technical Reports Server (NTRS)

Biaggi-Labiosa, Azlin

2016-01-01

Present an overview of the Nanotechnology Project at NASA's Game Changing Technology Industry Day. Mature and demonstrate flight readiness of CNT reinforced composites for future NASA mission applications?Sounding rocket test in a multiexperiment payload?Integrate into cold gas thruster system as propellant storage?The technology would provide the means for reduced COPV mass and improved damage tolerance and flight qualify CNT reinforced composites. PROBLEM/NEED BEING ADDRESSED:?Reduce weight and enhance the performance and damage tolerance of aerospace structuresGAME-CHANGING SOLUTION:?Improve mechanical properties of CNTs to eventually replace CFRP –lighter and stronger?First flight-testing of a CNT reinforced composite structural component as part of an operational flight systemUNIQUENESS:?CNT manufacturing methods developed?Flight qualify CNT reinforced composites

Improving the Fatigue Crack Propagation Resistance and Damage Tolerance of 2524-T3 Alloy with Amorphous Electroless Ni-P Coating

NASA Astrophysics Data System (ADS)

Chen, Lai; Zeng, Diping; Liu, Zhiyi; Bai, Song; Li, Junlin

2018-02-01

The surface microhardness, as well as the fatigue crack propagation (FCP) resistance of 2524-T3 alloy, is improved by producing a 20-μm-thick amorphous electroless Ni-12% P coating on its surface. Compared to the substrate, this deposited EN coating possesses higher strength properties and exhibits a greater ability of accommodating the plastic deformation at the fatigue crack tip, thereby remarkably improving the FCP resistance in near-threshold and early Paris regimes. Regardless of the similar FCP rates in Paris regime (Δ K ≥ 16.2 MPa m0.5), the coated sample exhibits extended Paris regime and enhanced damage tolerance.

Dynamics Impact Tolerance of Shuttle RCC Leading Edge Panels Using LS-DYNA

NASA Technical Reports Server (NTRS)

Fasanella, Edwin L.; Jackson, Karen E.; Lyle, Karen H.; Jones, Lisa E.; Hardy, Robin C.; Spellman, Regina L.; Carney, Kelly S.; Melis, Matthew E.; Stockwell, Alan E.

2005-01-01

This paper describes a research program conducted to enable accurate prediction of the impact tolerance of the shuttle Orbiter leading-edge wing panels using physics-based codes such as LS-DYNA, a nonlinear, explicit transient dynamic finite element code. The shuttle leading-edge panels are constructed of Reinforced-Carbon-Carbon (RCC) composite material, which is used because of its thermal properties to protect the shuttle during reentry into the Earth's atmosphere. Accurate predictions of impact damage from insulating foam and other debris strikes that occur during launch required materials characterization of expected debris, including strain-rate effects. First, analytical models of individual foam and RCC materials were validated. Next, analytical models of foam cylinders impacting 6- in. x 6-in. RCC flat plates were developed and validated. LS-DYNA pre-test models of the RCC flat plate specimens established the impact velocity of the test for three damage levels: no-detectable damage, non-destructive evaluation (NDE) detectable damage, or visible damage such as a through crack or hole. Finally, the threshold of impact damage for RCC on representative Orbiter wing panels was predicted for both a small through crack and for NDE-detectable damage.

Dynamic Impact Tolerance of Shuttle RCC Leading Edge Panels using LS-DYNA

NASA Technical Reports Server (NTRS)

Fasanella, Edwin; Jackson, Karen E.; Lyle, Karen H.; Jones, Lisa E.; Hardy, Robin C.; Spellman, Regina L.; Carney, Kelly S.; Melis, Matthew E.; Stockwell, Alan E.

2008-01-01

This paper describes a research program conducted to enable accurate prediction of the impact tolerance of the shuttle Orbiter leading-edge wing panels using 'physics-based- codes such as LS-DYNA, a nonlinear, explicit transient dynamic finite element code. The shuttle leading-edge panels are constructed of Reinforced-Carbon-Carbon (RCC) composite material, which issued because of its thermal properties to protect the shuttle during re-entry into the Earth's atmosphere. Accurate predictions of impact damage from insulating foam and other debris strikes that occur during launch required materials characterization of expected debris, including strain-rate effects. First, analytical models of individual foam and RCC materials were validated. Next, analytical models of individual foam cylinders impacting 6-in. x 6-in. RCC flat plates were developed and validated. LS-DYNA pre-test models of the RCC flat plate specimens established the impact velocity of the test for three damage levels: no-detectable damage, non-destructive evaluation (NDE) detectable damage, or visible damage such as a through crack or hole. Finally, the threshold of impact damage for RCC on representative Orbiter wing panels was predicted for both a small through crack and for NDE-detectable damage.

77 FR 4890 - Damage Tolerance and Fatigue Evaluation for Composite Rotorcraft Structures, and Damage Tolerance...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-01

...-AJ52, 2120-AJ51 Damage Tolerance and Fatigue Evaluation for Composite Rotorcraft Structures, and Damage... Tolerance and Fatigue Evaluation for Composite Rotorcraft Structures'' (76 FR 74655), published December 1... December 2, 2011. In the ``Composite Rotorcraft Structures'' rule, the FAA amended its regulations to...

Radiation Tolerant Interfaces: Influence of Local Stoichiometry at the Misfit Dislocation on Radiation Damage Resistance of Metal/Oxide Interfaces

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shutthanandan, Vaithiyalingam; Choudhury, Samrat; Manandhar, Sandeep

To understand how variations in interface properties such as misfit-dislocation density and local chemistry affect radiation-induced defect absorption and recombination, we have explored a model system of CrxV1-x alloy epitaxial films deposited on MgO single crystals. By controlling film composition, the lattice mismatch with MgO was adjusted so that the misfit-dislocation density varies at the interface. These interfaces were exposed to irradiation and in situ results show that the film with a semi-coherent interface (Cr) withstands irradiation while V film, which has similar semi-coherent interface like Cr, showed the largest damage. Theoretical calculations indicate that, unlike at metal/metal interfaces, themore » misfit dislocation density does not dominate radiation damage tolerance at metal/oxide interfaces. Rather, the stoichiometry, and the precise location of the misfit-dislocation density relative to the interface, drives defect behavior. Together, these results demonstrate the sensitivity of defect recombination to interfacial chemistry and provide new avenues for engineering radiation-tolerant nanomaterials.« less

Unprecedented simultaneous enhancement in damage tolerance and fatigue resistance of zirconia/Ta composites

NASA Astrophysics Data System (ADS)

Smirnov, A.; Beltrán, J. I.; Rodriguez-Suarez, T.; Pecharromán, C.; Muñoz, M. C.; Moya, J. S.; Bartolomé, J. F.

2017-03-01

Dense (>98 th%) and homogeneous ceramic/metal composites were obtained by spark plasma sintering (SPS) using ZrO2 and lamellar metallic powders of tantalum or niobium (20 vol.%) as starting materials. The present study has demonstrated the unique and unpredicted simultaneous enhancement in toughness and strength with very high flaw tolerance of zirconia/Ta composites. In addition to their excellent static mechanical properties, these composites also have exceptional resistance to fatigue loading. It has been shown that the major contributions to toughening are the resulting crack bridging and plastic deformation of the metallic particles, together with crack deflection and interfacial debonding, which is compatible with the coexistence in the composite of both, strong and weak ceramic/metal interfaces, in agreement with predictions of ab-initio calculations. Therefore, these materials are promising candidates for designing damage tolerance components for aerospace industry, cutting and drilling tools, biomedical implants, among many others.

Unprecedented simultaneous enhancement in damage tolerance and fatigue resistance of zirconia/Ta composites

PubMed Central

Smirnov, A.; Beltrán, J. I.; Rodriguez-Suarez, T.; Pecharromán, C.; Muñoz, M. C.; Moya, J. S.; Bartolomé, J. F.

2017-01-01

Dense (>98 th%) and homogeneous ceramic/metal composites were obtained by spark plasma sintering (SPS) using ZrO2 and lamellar metallic powders of tantalum or niobium (20 vol.%) as starting materials. The present study has demonstrated the unique and unpredicted simultaneous enhancement in toughness and strength with very high flaw tolerance of zirconia/Ta composites. In addition to their excellent static mechanical properties, these composites also have exceptional resistance to fatigue loading. It has been shown that the major contributions to toughening are the resulting crack bridging and plastic deformation of the metallic particles, together with crack deflection and interfacial debonding, which is compatible with the coexistence in the composite of both, strong and weak ceramic/metal interfaces, in agreement with predictions of ab-initio calculations. Therefore, these materials are promising candidates for designing damage tolerance components for aerospace industry, cutting and drilling tools, biomedical implants, among many others. PMID:28322343

Nanoindentation investigation of heavy ion irradiated Ti 3(Si,Al)C 2

NASA Astrophysics Data System (ADS)

Liu, X. M.; Le Flem, M.; Béchade, J. L.; Monnet, I.

2010-06-01

Because of good damage tolerance, thermal stability and interesting mechanical properties, Ti 3SiC 2, belonging to M n+1AX n phases, has been considered as a potential candidate material for applications in the future Gas Fast nuclear Reactors (GFR) such as components of fuel cladding working between 500 °C and 800 °C. However, the outstanding mechanical properties of Ti 3SiC 2 related to a layered microstructure could be impacted by irradiation. In this work, high energy Kr and Xe ion irradiated Ti 3Si 0.95Al 0.05C 2 and Ti 3Si 0.90Al 0.10C 2 samples, provided by IMR Shenyang, Chinese Academy of Science, were characterized by nanoindentation technique. After irradiation at room temperature, an increase in hardness with irradiation dose was highlighted. Nevertheless, some damage tolerance remained because of preservation of the typical MAX layered structure. Irradiations at 300 °C and 500 °C lead to less significant increase suggesting irradiation defect annealing. A complete recovery of the properties at 800 °C seems to be obtained.

Nano-enhanced aerospace composites for increased damage tolerance and service life damage monitoring

NASA Astrophysics Data System (ADS)

Paipetis, A.; Matikas, T. E.; Barkoula, N. M.; Karapappas, P.; Vavouliotis, A.; Kostopoulos, V.

2009-03-01

This study deals with new generation composite systems which apart from the primary reinforcement at the typical fiber scale (~10 μm) are also reinforced at the nanoscale. This is performed via incorporation of nano-scale additives in typical aerospace matrix systems, such as epoxies. Carbon Nanotubes (CNTs) are ideal candidates as their extremely high aspect ratio and mechanical properties render them advantageous to other nanoscale materials. The result is the significant increase in the damage tolerance of the novel composite systems even at very low CNT loadings. By monitoring the resistance change of the CNT network, information both on the real time deformation state of the composite is obtained as a reversible change in the bulk resistance of the material, and the damage state of the material as an irreversible change in the bulk resistance of the material. The irreversible monotonic increase of the electrical resistance can be related to internal damage in the hybrid composite system and may be used as an index of the remaining lifetime of a structural component.

Damage modeling of small-scale experiments on dental enamel with hierarchical microstructure.

PubMed

Scheider, I; Xiao, T; Yilmaz, E; Schneider, G A; Huber, N; Bargmann, S

2015-03-01

Dental enamel is a highly anisotropic and heterogeneous material, which exhibits an optimal reliability with respect to the various loads occurring over years. In this work, enamel's microstructure of parallel aligned rods of mineral fibers is modeled and mechanical properties are evaluated in terms of strength and toughness with the help of a multiscale modeling method. The established model is validated by comparing it with the stress-strain curves identified by microcantilever beam experiments extracted from these rods. Moreover, in order to gain further insight in the damage-tolerant behavior of enamel, the size of crystallites below which the structure becomes insensitive to flaws is studied by a microstructural finite element model. The assumption regarding the fiber strength is verified by a numerical study leading to accordance of fiber size and flaw tolerance size, and the debonding strength is estimated by optimizing the failure behavior of the microstructure on the hierarchical level above the individual fibers. Based on these well-grounded properties, the material behavior is predicted well by homogenization of a representative unit cell including damage, taking imperfections (like microcracks in the present case) into account. Copyright © 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Accelerated Insertion of Materials - Composites

DTIC Science & Technology

2001-08-28

Details • Damage Tolerance • Repair • Validation of Analysis Methodology • Fatigue • Static • Acoustic • Configuration Details • Damage Tolerance...Sensitivity – Fatigue – Adhesion – Damage Tolerance – All critical modes and environments Products: Material Specifications, B-Basis Design Allowables...Demonstrate damage tolerance AIM-C DARPA DARPA Workshop, Annapolis, August 27-28, 2001 Requalification of Polymer / Composite Parts • Material Changes – Raw

Computational Simulation of Composite Structural Fatigue

NASA Technical Reports Server (NTRS)

Minnetyan, Levon; Chamis, Christos C. (Technical Monitor)

2005-01-01

Progressive damage and fracture of composite structures subjected to monotonically increasing static, tension-tension cyclic, pressurization, and flexural cyclic loading are evaluated via computational simulation. Constituent material properties, stress and strain limits are scaled up to the structure level to evaluate the overall damage and fracture propagation for composites. Damage initiation, growth, accumulation, and propagation to fracture due to monotonically increasing static and cyclic loads are included in the simulations. Results show the number of cycles to failure at different temperatures and the damage progression sequence during different degradation stages. A procedure is outlined for use of computational simulation data in the assessment of damage tolerance, determination of sensitive parameters affecting fracture, and interpretation of results with insight for design decisions.

Computational Simulation of Composite Structural Fatigue

NASA Technical Reports Server (NTRS)

Minnetyan, Levon

2004-01-01

Progressive damage and fracture of composite structures subjected to monotonically increasing static, tension-tension cyclic, pressurization, and flexural cyclic loading are evaluated via computational simulation. Constituent material properties, stress and strain limits are scaled up to the structure level to evaluate the overall damage and fracture propagation for composites. Damage initiation, growth, accumulation, and propagation to fracture due to monotonically increasing static and cyclic loads are included in the simulations. Results show the number of cycles to failure at different temperatures and the damage progression sequence during different degradation stages. A procedure is outlined for use of computational simulation data in the assessment of damage tolerance, determination of sensitive parameters affecting fracture, and interpretation of results with insight for design decisions.

Damage-Survivable and Damage-Tolerant Laminated Composites with Optimally Placed Piezoelectric Layers

DTIC Science & Technology

1992-11-13

AD-A269 879 Damage-Survivable j and Damage-Tolerant Laminated Composites .4.. with Optimally placed Piezoelectric Layers Final Report No. 1 S. P...Damage Surviable and Damage-Tolerant Laminated Composites With Optimally Placed Piezoelectric Layers 12. PERSONAL AUTHOR(S) S.P. Joshi, W.S. Chan ൕa...block number) The main objective of the research is to assure that the embedded sensors/actuators in a smart laminated composite structure are damage

Damage tolerance assessment handbook. Volume 2 : airframe damage tolerance evaluation

DOT National Transportation Integrated Search

1999-02-01

The handbook is presented in two volumes. Volume I introduces the damage tolerance concept with an historical perspective followed by the fundamentals of fracture mechanics and fatigue crack propagation. Various fracture criteria and crack growth rul...

Multi-Functional Composite Fatigue

NASA Technical Reports Server (NTRS)

Minnetyan, Levon; Chamis, Christos C.

2008-01-01

Damage and fracture of composites subjected to monotonically increasing static, tension-tension cyclic, pressurization, and flexural cyclic loading are evaluated via a recently developed composite mechanics code that allows the user to focus on composite response at infinitely small scales. Constituent material properties, stress and strain limits are scaled up to the laminate level to evaluate the overall damage and durability. Results show the number of cycles to failure at different temperatures. A procedure is outlined for use of computational simulation data in the assessment of damage tolerance, determination of sensitive parameters affecting fracture, and interpretation of results with insight for design decisions.

Design, testing, and damage tolerance study of bonded stiffened composite wing cover panels

NASA Technical Reports Server (NTRS)

Madan, Ram C.; Sutton, Jason O.

1988-01-01

Results are presented from the application of damage tolerance criteria for composite panels to multistringer composite wing cover panels developed under NASA's Composite Transport Wing Technology Development contract. This conceptual wing design integrated aeroelastic stiffness constraints with an enhanced damage tolerance material system, in order to yield optimized producibility and structural performance. Damage tolerance was demonstrated in a test program using full-sized cover panel subcomponents; panel skins were impacted at midbay between stiffeners, directly over a stiffener, and over the stiffener flange edge. None of the impacts produced visible damage. NASTRAN analyses were performed to simulate NDI-detected invisible damage.

Damage tolerance certification of a fighter horizontal stabilizer

NASA Astrophysics Data System (ADS)

Huang, Jia-Yen; Tsai, Ming-Yang; Chen, Jong-Sheng; Ong, Ching-Long

1995-05-01

A review of the program for the damage tolerance certification test of a composite horizontal stabilizer (HS) of a fighter is presented. The object of this program is to certify that the fatigue life and damage tolerance strength of a damaged composite horizontal stabilizer meets the design requirements. According to the specification for damage tolerance certification, a test article should be subjected to two design lifetimes of flight-by-flight load spectra simulating the in-service fatigue loading condition for the aircraft. However, considering the effect of environmental change on the composite structure, one additional lifetime test was performed. In addition, to evaluate the possibilities for extending the service life of the structure, one more lifetime test was carried out with the spectrum increased by a factor of 1.4. To assess the feasibility and reliability of repair technology on a composite structure, two damaged areas were repaired after two lifetimes of damage tolerance test. On completion of four lifetimes of the damage tolerance test, the static residual strength was measured to check whether structural strength after repair met the requirements. Stiffness and static strength of the composite HS with and without damage were evaluated and compared.

Properties of five toughened matrix composite materials

NASA Technical Reports Server (NTRS)

Cano, Roberto J.; Dow, Marvin B.

1992-01-01

The use of toughened matrix composite materials offers an attractive solution to the problem of poor damage tolerance associated with advanced composite materials. In this study, the unidirectional laminate strengths and moduli, notched (open-hole) and unnotched tension and compression properties of quasi-isotropic laminates, and compression-after-impact strengths of five carbon fiber/toughened matrix composites, IM7/E7T1-2, IM7/X1845, G40-800X/5255-3, IM7/5255-3, and IM7/5260 have been evaluated. The compression-after-impact (CAI) strengths were determined primarily by impacting quasi-isotropic laminates with the NASA Langley air gun. A few CAI tests were also made with a drop-weight impactor. For a given impact energy, compression after impact strengths were determined to be dependent on impactor velocity. Properties and strengths for the five materials tested are compared with NASA data on other toughened matrix materials (IM7/8551-7, IM6/1808I, IM7/F655, and T800/F3900). This investigation found that all five materials were stronger and more impact damage tolerant than more brittle carbon/epoxy composite materials currently used in aircraft structures.

A Review of Australian and New Zealand Investigations on Aeronautical Fatigue During the Period April 2011 to March 2013

DTIC Science & Technology

2013-04-01

properties in high-strength coarse grain titanium alloy with rough and torturous fatigue surfaces - Kevin Walker, (DSTO and RMIT University Australia), and...Wanhill, R. J. H. (1995) Damage tolerance engineering property evaluations of aerospace aluminium alloys with emphasis on fatigue crack growth. National...T6/T651 alloys . The testing of 7075-T6 C(T) coupons confirmed that the properties are very similar to the 7075-T651 material. Baseline

Impact response of graphite/epoxy fabric structures

NASA Technical Reports Server (NTRS)

Lagace, Paul A.; Kraft, Michael J.

1990-01-01

The impact damage resistance and damage tolerance of graphite/epoxy fabric plate (coupon) and cylinder structures were investigated and compared in an analytical and experimental study. Hercules A370-5H/3501-6 five-harness satin weave cloth in a quasi-isotropic (0,45)(sub s) laminate configuration was utilized. Specimens were impacted with 12.7 mm diameter steel spheres at velocities ranging from 10 m/s to 100 m/s. Damage resistance of the specimens was determined through the use of dye penetrant enhanced x-radiography, sectioning, epoxy burnoff, and visual methods. Damage tolerance of the flat plate structures was assessed in a residual tensile test while damage tolerance of the cylinder structures was assessed via pressurization tests. Impacted fabric laminates exhibited matrix crushing, fiber breakage, delamination, and fiber bundle disbonds; the latter being a unique damage mode for fabric laminates. Plate delamination and bundle disbonding was found to be more extensive around the central core area of fiber damage in the coupon specimens than in the cylinder specimens which showed a cleaner damage area due to impact. Damage resistance and damage tolerance were predicted by utilizing a five-step analysis approach previously utilized for coupon configurations. Two of the five steps were adapted to account for the effects of the structural configuration of the pressurized cylinder. The damage resistance analysis provided good correlation to the fiber damage region of both the coupon and cylinder specimens. There was little difference in the size of this region in the two specimen types. However, the analysis was not able to predict the distribution of damage through-the-thickness. This was important in assessing the damage tolerance of the cylinders. The damage tolerance analysis was able to predict the residual tensile strength of the coupons. A general methodology to predict the impact damage resistance and damage tolerance of composite structures utilizing coupon data is presented.

Fuel containment and damage tolerance for large composite primary aircraft structures. Phase 1: Testing

NASA Technical Reports Server (NTRS)

Sandifer, J. P.

1983-01-01

Technical problems associated with fuel containment and damage tolerance of composite material wings for transport aircraft were identified. The major tasks are the following: (1) the preliminary design of damage tolerant wing surface using composite materials; (2) the evaluation of fuel sealing and lightning protection methods for a composite material wing; and (3) an experimental investigation of the damage tolerant characteristics of toughened resin graphite/epoxy materials. The test results, the test techniques, and the test data are presented.

Timing matters: error-prone gap filling and translesion synthesis in immunoglobulin gene hypermutation

PubMed Central

Sale, Julian E.; Batters, Christopher; Edmunds, Charlotte E.; Phillips, Lara G.; Simpson, Laura J.; Szüts, Dávid

2008-01-01

By temporarily deferring the repair of DNA lesions encountered during replication, the bypass of DNA damage is critical to the ability of cells to withstand genomic insults. Damage bypass can be achieved either by recombinational mechanisms that are generally accurate or by a process called translesion synthesis. Translesion synthesis involves replacing the stalled replicative polymerase with one of a number of specialized DNA polymerases whose active sites are able to tolerate a distorted or damaged DNA template. While this property allows the translesion polymerases to synthesize across damaged bases, it does so with the trade-off of an increased mutation rate. The deployment of these enzymes must therefore be carefully regulated. In addition to their important role in general DNA damage tolerance and mutagenesis, the translesion polymerases play a crucial role in converting the products of activation induced deaminase-catalysed cytidine deamination to mutations during immunoglobulin gene somatic hypermutation. In this paper, we specifically consider the control of translesion synthesis in the context of the timing of lesion bypass relative to replication fork progression and arrest at sites of DNA damage. We then examine how recent observations concerning the control of translesion synthesis might help refine our view of the mechanisms of immunoglobulin gene somatic hypermutation. PMID:19008194

The combined effect of glass buffer strips and stitching on the damage tolerance of composites

NASA Technical Reports Server (NTRS)

Kullerd, Susan M.

1993-01-01

Recent research has demonstrated that through-the-thickness stitching provides major improvements in the damage tolerance of composite laminates loaded in compression. However, the brittle nature of polymer matrix composites makes them susceptible to damage propagation, requiring special material applications and designs to limit damage growth. Glass buffer strips, embedded within laminates, have shown the potential for improving the damage tolerance of unstitched composite laminates loaded in tension. The glass buffer strips, less stiff than the surrounding carbon fibers, arrest crack growth in composites under tensile loads. The present study investigates the damage tolerance characteristics of laminates that contain both stitching and glass buffer strips.

Intraspecific competition facilitates the evolution of tolerance to insect damage in the perennial plant Solanum carolinense.

PubMed

McNutt, David W; Halpern, Stacey L; Barrows, Kahaili; Underwood, Nora

2012-12-01

Tolerance to herbivory (the degree to which plants maintain fitness after damage) is a key component of plant defense, so understanding how natural selection and evolutionary constraints act on tolerance traits is important to general theories of plant-herbivore interactions. These factors may be affected by plant competition, which often interacts with damage to influence trait expression and fitness. However, few studies have manipulated competitor density to examine the evolutionary effects of competition on tolerance. In this study, we tested whether intraspecific competition affects four aspects of the evolution of tolerance to herbivory in the perennial plant Solanum carolinense: phenotypic expression, expression of genetic variation, the adaptive value of tolerance, and costs of tolerance. We manipulated insect damage and intraspecific competition for clonal lines of S. carolinense in a greenhouse experiment, and measured tolerance in terms of sexual and asexual fitness components. Compared to plants growing at low density, plants growing at high density had greater expression of and genetic variation in tolerance, and experienced greater fitness benefits from tolerance when damaged. Tolerance was not costly for plants growing at either density, and only plants growing at low density benefited from tolerance when undamaged, perhaps due to greater intrinsic growth rates of more tolerant genotypes. These results suggest that competition is likely to facilitate the evolution of tolerance in S. carolinense, and perhaps in other plants that regularly experience competition, while spatio-temporal variation in density may maintain genetic variation in tolerance.

An overview of the NASA textile composites program

NASA Technical Reports Server (NTRS)

Dexter, H. Benson

1993-01-01

The NASA Langley Research Center is conducting and sponsoring research to explore the benefits of textile reinforced composites for civil transport aircraft primary structures. The objective of this program is to develop and demonstrate the potential of affordable textile reinforced composite materials to meet design properties and damage tolerance requirements of advanced aircraft structures. In addition to in-house research, the program includes major participation by the aircraft industry and aerospace textile companies. The major program elements include development of textile preforms, processing science, mechanics of materials, experimental characterization of materials, and development and evaluation of textile reinforced composite structural elements and subcomponents. The NASA Langley in-house research is focused on science-based understanding of resin transfer molding (RTM), development of powder-coated towpreg processes, analysis methodology, and development of a performance database on textile reinforced composites. The focus of the textile industry participation is on development of multidirectional, damage-tolerant preforms, and the aircraft industry participation is in the areas of innovative design concepts, cost-effective fabrication, and testing of textile reinforced composite structural elements and subcomponents. Textile processes such as 3-D weaving, 2-D and 3-D braiding, and knitting/stitching are being compared with conventional laminated tape processes for improved damage tolerance. Through-the-thickness reinforcements offer significant damage tolerance improvements. However, these gains must be weighed against potential loss in in-plane properties such as strength and stiffness. Analytical trade studies are underway to establish design guidelines for the application of textile material forms to meet specific loading requirements. Fabrication and testing of large structural components are required to establish the full potential of textile reinforced composite materials. The goals of the NASA Langley-sponsored research program are to demonstrate technology readiness with subscale composite components by 1995 and to verify the performance of full-scale composite primary aircraft structural components by 1997. The status of textile reinforced composite structural elements under development by Boeing, Douglas, Lockheed, and Grumman are presented. Included are braided frames and woven/stitched wing and fuselage panels.

Cancer cells recovering from damage exhibit mitochondrial restructuring and increased aerobic glycolysis.

PubMed

Akakura, Shin; Ostrakhovitch, Elena; Sanokawa-Akakura, Reiko; Tabibzadeh, Siamak

2014-06-13

Instead of relying on mitochondrial oxidative phosphorylation, most cancer cells rely heavily on aerobic glycolysis, a phenomenon termed as "the Warburg effect". We considered that this effect is a direct consequence of damage which persists in cancer cells that recover from damage. To this end, we studied glycolysis and rate of cell proliferation in cancer cells that recovered from severe damage. We show that in vitro Damage-Recovered (DR) cells exhibit mitochondrial structural remodeling, display Warburg effect, and show increased in vitro and in vivo proliferation and tolerance to damage. To test whether cancer cells derived from tumor microenvironment can show similar properties, we isolated Damage-Recovered (T(DR)) cells from tumors. We demonstrate that T(DR) cells also show increased aerobic glycolysis and a high proliferation rate. These findings show that Warburg effect and its consequences are induced in cancer cells that survive severe damage. Copyright © 2014 Elsevier Inc. All rights reserved.

Damage Tolerance of Composites

NASA Technical Reports Server (NTRS)

Hodge, Andy

2007-01-01

Fracture control requirements have been developed to address damage tolerance of composites for manned space flight hardware. The requirements provide the framework for critical and noncritical hardware assessment and testing. The need for damage threat assessments, impact damage protection plans, and nondestructive evaluation are also addressed. Hardware intended to be damage tolerant have extensive coupon, sub-element, and full-scale testing requirements in-line with the Building Block Approach concept from the MIL-HDBK-17, Department of Defense Composite Materials Handbook.

Damage Tolerance Analysis of a Pressurized Liquid Oxygen Tank

NASA Technical Reports Server (NTRS)

Forth, Scott C.; Harvin, Stephen F.; Gregory, Peyton B.; Mason, Brian H.; Thompson, Joe E.; Hoffman, Eric K.

2006-01-01

A damage tolerance assessment was conducted of an 8,000 gallon pressurized Liquid Oxygen (LOX) tank. The LOX tank is constructed of a stainless steel pressure vessel enclosed by a thermal-insulating vacuum jacket. The vessel is pressurized to 2,250 psi with gaseous nitrogen resulting in both thermal and pressure stresses on the tank wall. Finite element analyses were performed on the tank to characterize the stresses from operation. Engineering material data was found from both the construction of the tank and the technical literature. An initial damage state was assumed based on records of a nondestructive inspection performed on the tank. The damage tolerance analyses were conducted using the NASGRO computer code. This paper contains the assumptions, and justifications, made for the input parameters to the damage tolerance analyses and the results of the damage tolerance analyses with a discussion on the operational safety of the LOX tank.

14 CFR 23.574 - Metallic damage tolerance and fatigue evaluation of commuter category airplanes.

Code of Federal Regulations, 2014 CFR

2014-01-01

... evaluation of commuter category airplanes. 23.574 Section 23.574 Aeronautics and Space FEDERAL AVIATION... COMMUTER CATEGORY AIRPLANES Structure Fatigue Evaluation § 23.574 Metallic damage tolerance and fatigue evaluation of commuter category airplanes. For commuter category airplanes— (a) Metallic damage tolerance. An...

14 CFR 23.574 - Metallic damage tolerance and fatigue evaluation of commuter category airplanes.

Code of Federal Regulations, 2012 CFR

2012-01-01

... evaluation of commuter category airplanes. 23.574 Section 23.574 Aeronautics and Space FEDERAL AVIATION... COMMUTER CATEGORY AIRPLANES Structure Fatigue Evaluation § 23.574 Metallic damage tolerance and fatigue evaluation of commuter category airplanes. For commuter category airplanes— (a) Metallic damage tolerance. An...

14 CFR 23.574 - Metallic damage tolerance and fatigue evaluation of commuter category airplanes.

Code of Federal Regulations, 2013 CFR

2013-01-01

... evaluation of commuter category airplanes. 23.574 Section 23.574 Aeronautics and Space FEDERAL AVIATION... COMMUTER CATEGORY AIRPLANES Structure Fatigue Evaluation § 23.574 Metallic damage tolerance and fatigue evaluation of commuter category airplanes. For commuter category airplanes— (a) Metallic damage tolerance. An...

14 CFR 23.574 - Metallic damage tolerance and fatigue evaluation of commuter category airplanes.

Code of Federal Regulations, 2011 CFR

2011-01-01

... evaluation of commuter category airplanes. 23.574 Section 23.574 Aeronautics and Space FEDERAL AVIATION... COMMUTER CATEGORY AIRPLANES Structure Fatigue Evaluation § 23.574 Metallic damage tolerance and fatigue evaluation of commuter category airplanes. For commuter category airplanes— (a) Metallic damage tolerance. An...

14 CFR 23.574 - Metallic damage tolerance and fatigue evaluation of commuter category airplanes.

Code of Federal Regulations, 2010 CFR

2010-01-01

... evaluation of commuter category airplanes. 23.574 Section 23.574 Aeronautics and Space FEDERAL AVIATION... COMMUTER CATEGORY AIRPLANES Structure Fatigue Evaluation § 23.574 Metallic damage tolerance and fatigue evaluation of commuter category airplanes. For commuter category airplanes— (a) Metallic damage tolerance. An...

A Study of Damage Tolerance in Curved Composite Panels.

DTIC Science & Technology

1988-03-01

A198 617 A STUDY OF DAMAGE TOLERANCE IN CURVED COMPOSITE PANELS 1/2 I CNO O NGNERN BLNuE Hl(U) Ala FORCE INST OF TECH &IRIGHT-PATTERSON APR ON...OAL B PEAU OF STANOARDS- -T - % r . P.4 .% FILE CC R CDV OF DTIC MAR3 11988 *grr A STUDY OF DAMAGE TOLERANCE IN CURVED COMPOSITE PANELS THESIS...AFIT/GA/AA/88-.3 0 A STUDY OF DAMAGE TOLERANCE IN CURVED COMPOSITE PANELS THESIS - Brendan L. Wilder Captain, USAF S*2822 $$$*fltf$$*2*f$1fltf

Advanced information processing system - Status report. [for fault tolerant and damage tolerant data processing for aerospace vehicles

NASA Technical Reports Server (NTRS)

Brock, L. D.; Lala, J.

1986-01-01

The Advanced Information Processing System (AIPS) is designed to provide a fault tolerant and damage tolerant data processing architecture for a broad range of aerospace vehicles. The AIPS architecture also has attributes to enhance system effectiveness such as graceful degradation, growth and change tolerance, integrability, etc. Two key building blocks being developed by the AIPS program are a fault and damage tolerant processor and communication network. A proof-of-concept system is now being built and will be tested to demonstrate the validity and performance of the AIPS concepts.

Nanoscale origins of the damage tolerance of the high-entropy alloy CrMnFeCoNi

DOE PAGES

Zhang, ZiJiao; Mao, M. M.; Wang, Jiangwei; ...

2015-12-09

Damage tolerance can be an elusive characteristic of structural materials requiring both high strength and ductility, properties that are often mutually exclusive. High-entropy alloys are of interest in this regard. Specifically, the single-phase CrMnFeCoNi alloy displays tensile strength levels of ~1 GPa, excellent ductility (~60–70%) and exceptional fracture toughness (KJIc>200M Pa√m). Here through the use of in situ straining in an aberration-corrected transmission electron microscope, we report on the salient atomistic to micro-scale mechanisms underlying the origin of these properties. We identify a synergy of multiple deformation mechanisms, rarely achieved in metallic alloys, which generates high strength, work hardening andmore » ductility, including the easy motion of Shockley partials, their interactions to form stacking-fault parallelepipeds, and arrest at planar slip bands of undissociated dislocations. In conclusion, we further show that crack propagation is impeded by twinned, nanoscale bridges that form between the near-tip crack faces and delay fracture by shielding the crack tip.« less

Nanoscale origins of the damage tolerance of the high-entropy alloy CrMnFeCoNi

PubMed Central

Zhang, ZiJiao; Mao, M. M.; Wang, Jiangwei; Gludovatz, Bernd; Zhang, Ze; Mao, Scott X.; George, Easo P.; Yu, Qian; Ritchie, Robert O.

2015-01-01

Damage tolerance can be an elusive characteristic of structural materials requiring both high strength and ductility, properties that are often mutually exclusive. High-entropy alloys are of interest in this regard. Specifically, the single-phase CrMnFeCoNi alloy displays tensile strength levels of ∼1 GPa, excellent ductility (∼60–70%) and exceptional fracture toughness (KJIc>200 MPa√m). Here through the use of in situ straining in an aberration-corrected transmission electron microscope, we report on the salient atomistic to micro-scale mechanisms underlying the origin of these properties. We identify a synergy of multiple deformation mechanisms, rarely achieved in metallic alloys, which generates high strength, work hardening and ductility, including the easy motion of Shockley partials, their interactions to form stacking-fault parallelepipeds, and arrest at planar slip bands of undissociated dislocations. We further show that crack propagation is impeded by twinned, nanoscale bridges that form between the near-tip crack faces and delay fracture by shielding the crack tip. PMID:26647978

Structurally Integrated, Damage-Tolerant, Thermal Spray Coatings

NASA Astrophysics Data System (ADS)

Vackel, Andrew; Dwivedi, Gopal; Sampath, Sanjay

2015-07-01

Thermal spray coatings are used extensively for the protection and life extension of engineering components exposed to harsh wear and/or corrosion during service in aerospace, energy, and heavy machinery sectors. Cermet coatings applied via high-velocity thermal spray are used in aggressive wear situations almost always coupled with corrosive environments. In several instances (e.g., landing gear), coatings are considered as part of the structure requiring system-level considerations. Despite their widespread use, the technology has lacked generalized scientific principles for robust coating design, manufacturing, and performance analysis. Advances in process and in situ diagnostics have provided significant insights into the process-structure-property-performance correlations providing a framework-enhanced design. In this overview, critical aspects of materials, process, parametrics, and performance are discussed through exemplary studies on relevant compositions. The underlying connective theme is understanding and controlling residual stresses generation, which not only addresses process dynamics but also provides linkage for process-property relationship for both the system (e.g., fatigue) and the surface (wear and corrosion). The anisotropic microstructure also invokes the need for damage-tolerant material design to meet future goals.

Advanced Composite Wind Turbine Blade Design Based on Durability and Damage Tolerance

DOE Office of Scientific and Technical Information (OSTI.GOV)

Abumeri, Galib; Abdi, Frank

2012-02-16

The objective of the program was to demonstrate and verify Certification-by-Analysis (CBA) capability for wind turbine blades made from advanced lightweight composite materials. The approach integrated durability and damage tolerance analysis with robust design and virtual testing capabilities to deliver superior, durable, low weight, low cost, long life, and reliable wind blade design. The GENOA durability and life prediction software suite was be used as the primary simulation tool. First, a micromechanics-based computational approach was used to assess the durability of composite laminates with ply drop features commonly used in wind turbine applications. Ply drops occur in composite joints andmore » closures of wind turbine blades to reduce skin thicknesses along the blade span. They increase localized stress concentration, which may cause premature delamination failure in composite and reduced fatigue service life. Durability and damage tolerance (D&DT) were evaluated utilizing a multi-scale micro-macro progressive failure analysis (PFA) technique. PFA is finite element based and is capable of detecting all stages of material damage including initiation and propagation of delamination. It assesses multiple failure criteria and includes the effects of manufacturing anomalies (i.e., void, fiber waviness). Two different approaches have been used within PFA. The first approach is Virtual Crack Closure Technique (VCCT) PFA while the second one is strength-based. Constituent stiffness and strength properties for glass and carbon based material systems were reverse engineered for use in D&DT evaluation of coupons with ply drops under static loading. Lamina and laminate properties calculated using manufacturing and composite architecture details matched closely published test data. Similarly, resin properties were determined for fatigue life calculation. The simulation not only reproduced static strength and fatigue life as observed in the test, it also showed composite damage and fracture modes that resemble those reported in the tests. The results show that computational simulation can be relied on to enhance the design of tapered composite structures such as the ones used in turbine wind blades. A computational simulation for durability, damage tolerance (D&DT) and reliability of composite wind turbine blade structures in presence of uncertainties in material properties was performed. A composite turbine blade was first assessed with finite element based multi-scale progressive failure analysis to determine failure modes and locations as well as the fracture load. D&DT analyses were then validated with static test performed at Sandia National Laboratories. The work was followed by detailed weight analysis to identify contribution of various materials to the overall weight of the blade. The methodology ensured that certain types of failure modes, such as delamination progression, are contained to reduce risk to the structure. Probabilistic analysis indicated that composite shear strength has a great influence on the blade ultimate load under static loading. Weight was reduced by 12% with robust design without loss in reliability or D&DT. Structural benefits obtained with the use of enhanced matrix properties through nanoparticles infusion were also assessed. Thin unidirectional fiberglass layers enriched with silica nanoparticles were applied to the outer surfaces of a wind blade to improve its overall structural performance and durability. The wind blade was a 9-meter prototype structure manufactured and tested subject to three saddle static loading at Sandia National Laboratory (SNL). The blade manufacturing did not include the use of any nano-material. With silica nanoparticles in glass composite applied to the exterior surfaces of the blade, the durability and damage tolerance (D&DT) results from multi-scale PFA showed an increase in ultimate load of the blade by 9.2% as compared to baseline structural performance (without nano). The use of nanoparticles lead to a delay in the onset of delamination. Load-displacement relationships obtained from testing of the blade with baseline neat material were compared to the ones from analytical simulation using neat resin and using silica nanoparticles in the resin. Multi-scale PFA results for the neat material construction matched closely those from test for both load displacement and location and type of damage and failure. AlphaSTAR demonstrated that wind blade structures made from advanced composite materials can be certified with multi-scale progressive failure analysis by following building block verification approach.« less

Ideal sinks are not always ideal. Radiation damage accumulation in nanocomposites

DOE PAGES

Uberuaga, Blas Pedro; Choudhury, Samrat; Caro, Alfredo

2014-11-27

Designing radiation tolerant materials is one of the primary challenges associated with advanced nuclear energy systems. One attractive route that has received much attention world-wide is to introduce a high density of sinks, often in the form of interfaces or secondary phases. Here, we develop a simple model of such nanocomposites and examine the ramifications of various factors on the overall radiation stability of the material. In particular, we determine how the distribution of secondary phases, the relative sink strength of those phases, and the irradiation temperature influence the radiation tolerance of the matrix. We find that the best scenariomore » is one in which the sinks have intermediate strength, transiently trapping defects before releasing them back into the matrix.This provides new insight into the optimal properties of nanocomposites for radiation damage environments.« less

Water availability limits tolerance of apical damage in the Chilean tarweed Madia sativa

NASA Astrophysics Data System (ADS)

Gonzáles, Wilfredo L.; Suárez, Lorena H.; Molina-Montenegro, Marco A.; Gianoli, Ernesto

2008-07-01

Plant tolerance is the ability to reduce the negative impact of herbivory on plant fitness. Numerous studies have shown that plant tolerance is affected by nutrient availability, but the effect of soil moisture has received less attention. We evaluated tolerance of apical damage (clipping that mimicked insect damage) under two watering regimes (control watering and drought) in the tarweed Madia sativa (Asteraceae). We recorded number of heads with seeds and total number of heads as traits related to fitness. Net photosynthetic rate, water use efficiency, number of branches, shoot biomass, and the root:shoot biomass ratio were measured as traits potentially related to tolerance via compensatory responses to damage. In the drought treatment, damaged plants showed ≈43% reduction in reproductive fitness components in comparison with undamaged plants. In contrast, there was no significant difference in reproductive fitness between undamaged and damaged plants in the control watering treatment. Shoot biomass was not affected by apical damage. The number of branches increased after damage in both water treatments but this increase was limited by drought stress. Net photosynthetic rate increased in damaged plants only in the control watering treatment. Water use efficiency increased with drought stress and, in plants regularly watered, also increased after damage. Root:shoot ratio was higher in the low water treatment and damaged plants tended to reduce root:shoot ratio only in this water treatment. It is concluded that water availability limits tolerance to apical damage in M. sativa, and that putative compensatory mechanisms are differentially affected by water availability.

75 FR 24502 - Damage Tolerance and Fatigue Evaluation of Composite Rotorcraft Structures; Reopening of Comment...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-05

.... FAA-2009-0660; Notice No. 10-09] RIN 2120-AJ52 Damage Tolerance and Fatigue Evaluation of Composite... requirements of normal and transport category rotorcraft. The amendment would address advances in composite... 793) Notice No. 09-12, entitled ``Damage Tolerance and Fatigue Evaluation of Composite Rotorcraft...

Concepts for improving the damage tolerance of composite compression panels. [aircraft structures

NASA Technical Reports Server (NTRS)

Rhodes, M. D.; Williams, J. G.

1984-01-01

The residual strength of specimens with damage and the sensitivity to damage while subjected to an applied inplane compression load were determined for flatplate specimens and blade-stiffened panels. The results suggest that matrix materials that fail by delamination have the lowest damage tolerance capability. Alternate matrix materials or laminates which are transversely reinforced suppress the delamination mode of failure and change the failure mode to transverse shear crippling which occurs at a higher strain value. Several damage-tolerant blade-stiffened panel design concepts are evaluated. Structural efficiency studies conducted show only small mass penalties may result from incorporating these damage-tolerant features in panel design. The implication of test results on the design of aircraft structures was examined with respect to FAR requirements.

Phosphorylation of human INO80 is involved in DNA damage tolerance

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kato, Dai; Waki, Mayumi; Umezawa, Masaki

Highlights: Black-Right-Pointing-Pointer Depletion of hINO80 significantly reduced PCNA ubiquitination. Black-Right-Pointing-Pointer Depletion of hINO80 significantly reduced nuclear dots intensity of RAD18 after UV irradiation. Black-Right-Pointing-Pointer Western blot analyses showed phosphorylated hINO80 C-terminus. Black-Right-Pointing-Pointer Overexpression of phosphorylation mutant hINO80 reduced PCNA ubiquitination. -- Abstract: Double strand breaks (DSBs) are the most serious type of DNA damage. DSBs can be generated directly by exposure to ionizing radiation or indirectly by replication fork collapse. The DNA damage tolerance pathway, which is conserved from bacteria to humans, prevents this collapse by overcoming replication blockages. The INO80 chromatin remodeling complex plays an important role in themore » DNA damage response. The yeast INO80 complex participates in the DNA damage tolerance pathway. The mechanisms regulating yINO80 complex are not fully understood, but yeast INO80 complex are necessary for efficient proliferating cell nuclear antigen (PCNA) ubiquitination and for recruitment of Rad18 to replication forks. In contrast, the function of the mammalian INO80 complex in DNA damage tolerance is less clear. Here, we show that human INO80 was necessary for PCNA ubiquitination and recruitment of Rad18 to DNA damage sites. Moreover, the C-terminal region of human INO80 was phosphorylated, and overexpression of a phosphorylation-deficient mutant of human INO80 resulted in decreased ubiquitination of PCNA during DNA replication. These results suggest that the human INO80 complex, like the yeast complex, was involved in the DNA damage tolerance pathway and that phosphorylation of human INO80 was involved in the DNA damage tolerance pathway. These findings provide new insights into the DNA damage tolerance pathway in mammalian cells.« less

Electrochemical activity evaluation of chemically damaged carbon nanotube with palladium nanoparticles for ethanol oxidation

NASA Astrophysics Data System (ADS)

Ahmed, Mohammad Shamsuddin; Jeon, Seungwon

2015-05-01

The carbon nanotube (CNT) has unique electrical and structural properties due to it's sp2 π-conjugative structure that leads to the higher electrocatalysis. The π-conjugative structure, that allows the CNT interact with various compounds and metal nanoparticles (NPs) through π-π electronic interaction. However, the damage of π-conjugative sidewall of CNT that can be hinder the electrocatalytic activity has found. For this study, the CNT, as base material, has been prepared through a conventional acid treatment method up to 15 h; the higher degree of sidewall damage has been observed in last 5 h during treatment period. The short and long term acid treated (denoted as CNT and CNT-COOH, respectively) CNTs have been subsequently fabricated with palladium NPs (denoted as CNT/Pd and CNT-Pd, respectively) and employed as ethanol oxidation reaction (EOR) catalysts. The CNT-Pd displays a poor electrocatalytic performance towards EOR than that of CNT/Pd due to the damage of π-conjugative sidewall. The kinetic parameters including poisoning tolerance have also been hampered by the surface damage. The CNT/Pd (∼3.3 folds) and CNT-Pd (∼1.5 folds) are express higher electrocatalytic activity and poisoning tolerance than that of Pd/C while Pd mass loading remains in the same amount.

Effect of water availability on tolerance of leaf damage in tall morning glory, Ipomoea purpurea

NASA Astrophysics Data System (ADS)

Atala, Cristian; Gianoli, Ernesto

2009-03-01

Resource availability may limit plant tolerance of herbivory. To predict the effect of differential resource availability on plant tolerance, the limiting resource model (LRM) considers which resource limits plant fitness and which resource is mostly affected by herbivore damage. We tested the effect of experimental drought on tolerance of leaf damage in Ipomoea purpurea, which is naturally exposed to both leaf damage and summer drought. To seek mechanistic explanations, we also measured several morphological, allocation and gas exchange traits. In this case, LRM predicts that tolerance would be the same in both water treatments. Plants were assigned to a combination of two water treatments (control and low water) and two damage treatments (50% defoliation and undamaged). Plants showed tolerance of leaf damage, i.e., a similar number of fruits were produced by damaged and undamaged plants, only in control water. Whereas experimental drought affected all plant traits, leaf damage caused plants to show a greater leaf trichome density and reduced shoot biomass, but only in low water. It is suggested that the reduced fitness (number of fruits) of damaged plants in low water was mediated by the differential reduction of shoot biomass, because the number of fruits per shoot biomass was similar in damaged and undamaged plants. Alternative but less likely explanations include the opposing direction of functional responses to drought and defoliation, and resource costs of the damage-induced leaf trichome density. Our results somewhat challenge the LRM predictions, but further research including field experiments is needed to validate some of the preliminary conclusions drawn.

Progressive Fracture and Damage Tolerance of Composite Pressure Vessels

NASA Technical Reports Server (NTRS)

Chamis, Christos C.; Gotsis, Pascal K.; Minnetyan, Levon

1997-01-01

Structural performance (integrity, durability and damage tolerance) of fiber reinforced composite pressure vessels, designed for pressured shelters for planetary exploration, is investigated via computational simulation. An integrated computer code is utilized for the simulation of damage initiation, growth, and propagation under pressure. Aramid fibers are considered in a rubbery polymer matrix for the composite system. Effects of fiber orientation and fabrication defect/accidental damages are investigated with regard to the safety and durability of the shelter. Results show the viability of fiber reinforced pressure vessels as damage tolerant shelters for planetary colonization.

Ontogenetic patterns in the mechanisms of tolerance to herbivory in Plantago

PubMed Central

Barton, Kasey E.

2013-01-01

Background and Aims Herbivory and plant defence differ markedly among seedlings and juvenile and mature plants in most species. While ontogenetic patterns of chemical resistance have been the focus of much research, comparatively little is known about how tolerance to damage changes across ontogeny. Due to dramatic shifts in plant size, resource acquisition, stored reserves and growth, it was predicted that tolerance and related underlying mechanisms would differ among ontogenetic stages. Methods Ontogenetic patterns in the mechanisms of tolerance were investigated in Plantago lanceolata and P. major (Plantaginaceae) using the genetic sib-ship approach. Pot-grown plants were subjected to 50 % defoliation at the seedling, juvenile and mature stages and either harvested in the short-term to look at plasticity in growth and photosynthesis in response to damage or allowed to grow through seed maturation to measure phenology, shoot compensation and reproductive fitness. Key Results Tolerance to defoliation was high in P. lanceolata, but low in P. major, and did not vary among ontogenetic stages in either species. Mechanisms underlying tolerance did vary across ontogeny. In P. lanceolata, tolerance was significantly related to flowering (juveniles) and pre-damage shoot biomass (mature plants). In P. major, tolerance was significantly related to pre-damage root biomass (seedlings) and induction of non-photochemical quenching, a photosynthetic parameter (juveniles). Conclusions Biomass partitioning was very plastic in response to damage and showed associations with tolerance in both species, indicating a strong role in plant defence. In contrast, photosynthesis and phenology showed weaker responses to damage and were related to tolerance only in certain ontogenetic stages. This study highlights the pivotal role of ontogeny in plant defence and herbivory. Additional studies in more species are needed to determine how seedlings tolerate herbivory in general and whether mechanisms vary across ontogeny in consistent patterns. PMID:23589631

Multiaxial and thermomechanical fatigue considerations in damage tolerant design

NASA Technical Reports Server (NTRS)

Leese, G. E.; Bill, R. C.

1985-01-01

In considering damage tolerant design concepts for gas turbine hot section components, several challenging concerns arise: Complex multiaxial loading situations are encountered; Thermomechanical fatigue loading involving very wide temperature ranges is imposed on components; Some hot section materials are extremely anisotropic; and coatings and environmental interactions play an important role in crack propagation. The effects of multiaxiality and thermomechanical fatigue are considered from the standpoint of their impact on damage tolerant design concepts. Recently obtained research results as well as results from the open literature are examined and their implications for damage tolerant design are discussed. Three important needs required to advance analytical capabilities in support of damage tolerant design become readily apparent: (1) a theoretical basis to account for the effect of nonproportional loading (mechanical and mechanical/thermal); (2) the development of practical crack growth parameters that are applicable to thermomechanical fatigue situations; and (3) the development of crack growth models that address multiple crack failures.

Electronic hybridisation implications for the damage-tolerance of thin film metallic glasses

PubMed Central

Schnabel, Volker; Jaya, B. Nagamani; Köhler, Mathias; Music, Denis; Kirchlechner, Christoph; Dehm, Gerhard; Raabe, Dierk; Schneider, Jochen M.

2016-01-01

A paramount challenge in materials science is to design damage-tolerant glasses. Poisson’s ratio is commonly used as a criterion to gauge the brittle-ductile transition in glasses. However, our data, as well as results in the literature, are in conflict with the concept of Poisson’s ratio serving as a universal parameter for fracture energy. Here, we identify the electronic structure fingerprint associated with damage tolerance in thin film metallic glasses. Our correlative theoretical and experimental data reveal that the fraction of bonds stemming from hybridised states compared to the overall bonding can be associated with damage tolerance in thin film metallic glasses. PMID:27819318

Electronic hybridisation implications for the damage-tolerance of thin film metallic glasses.

PubMed

Schnabel, Volker; Jaya, B Nagamani; Köhler, Mathias; Music, Denis; Kirchlechner, Christoph; Dehm, Gerhard; Raabe, Dierk; Schneider, Jochen M

2016-11-07

A paramount challenge in materials science is to design damage-tolerant glasses. Poisson's ratio is commonly used as a criterion to gauge the brittle-ductile transition in glasses. However, our data, as well as results in the literature, are in conflict with the concept of Poisson's ratio serving as a universal parameter for fracture energy. Here, we identify the electronic structure fingerprint associated with damage tolerance in thin film metallic glasses. Our correlative theoretical and experimental data reveal that the fraction of bonds stemming from hybridised states compared to the overall bonding can be associated with damage tolerance in thin film metallic glasses.

A modal H∞-norm-based performance requirement for damage-tolerant active controller design

NASA Astrophysics Data System (ADS)

Genari, Helói F. G.; Mechbal, Nazih; Coffignal, Gérard; Nóbrega, Eurípedes G. O.

2017-04-01

Damage-tolerant active control (DTAC) is a recent research area that encompasses control design methodologies resulting from the application of fault-tolerant control methods to vibration control of structures subject to damage. The possibility of damage occurrence is not usually considered in the active vibration control design requirements. Damage changes the structure dynamics, which may produce unexpected modal behavior of the closed-loop system, usually not anticipated by the controller design approaches. A modal H∞ norm and a respective robust controller design framework were recently introduced, and this method is here extended to face a new DTAC strategy implementation. Considering that damage affects each vibration mode differently, this paper adopts the modal H∞ norm to include damage as a design requirement. The basic idea is to create an appropriate energy distribution over the frequency range of interest and respective vibration modes, guaranteeing robustness, damage tolerance, and adequate overall performance, taking into account that it is common to have previous knowledge of the structure regions where damage may occur during its operational life. For this purpose, a structural health monitoring technique is applied to evaluate modal modifications caused by damage. This information is used to create modal weighing matrices, conducting to the modal H∞ controller design. Finite element models are adopted for a case study structure, including different damage severities, in order to validate the proposed control strategy. Results show the effectiveness of the proposed methodology with respect to damage tolerance.

Concepts for improving the damage tolerance of composite compression panels

NASA Technical Reports Server (NTRS)

Rhodes, M. D.; Williams, J. G.

1981-01-01

The results of an experimental evaluation of graphite-epoxy composite compression panel impact damage tolerance and damage propagation arrest concepts are reported. The tests were conducted on flat plate specimens and blade-stiffened structural panels such as those used in commercial aircraft wings, and the residual strength of damaged specimens and their sensitivity to damage while subjected to in-plane compression loading were determined. Results suggest that matrix materials that fail by delamination have the lowest damage tolerance, and it is concluded that alternative matrix materials with transverse reinforcement to suppress the delamination failure mode and yield the higher-strain value transverse shear crippling mode should be developed.

Ultraviolet-B-induced DNA damage and ultraviolet-B tolerance mechanisms in species with different functional groups coexisting in subalpine moorlands.

PubMed

Wang, Qing-Wei; Kamiyama, Chiho; Hidema, Jun; Hikosaka, Kouki

2016-08-01

High doses of ultraviolet-B (UV-B; 280-315 nm) radiation can have detrimental effects on plants, and especially damage their DNA. Plants have DNA repair and protection mechanisms to prevent UV-B damage. However, it remains unclear how DNA damage and tolerance mechanisms vary among field species. We studied DNA damage and tolerance mechanisms in 26 species with different functional groups coexisting in two moorlands at two elevations. We collected current-year leaves in July and August, and determined accumulation of cyclobutane pyrimidine dimer (CPD) as UV-B damage and photorepair activity (PRA) and concentrations of UV-absorbing compounds (UACs) and carotenoids (CARs) as UV-B tolerance mechanisms. DNA damage was greater in dicot than in monocot species, and higher in herbaceous than in woody species. Evergreen species accumulated more CPDs than deciduous species. PRA was higher in Poaceae than in species of other families. UACs were significantly higher in woody than in herbaceous species. The CPD level was not explained by the mechanisms across species, but was significantly related to PRA and UACs when we ignored species with low CPD, PRA and UACs, implying the presence of another effective tolerance mechanism. UACs were correlated negatively with PRA and positively with CARs. Our results revealed that UV-induced DNA damage significantly varies among native species, and this variation is related to functional groups. DNA repair, rather than UV-B protection, dominates in UV-B tolerance in the field. Our findings also suggest that UV-B tolerance mechanisms vary among species under evolutionary trade-off and synergism.

Properties of three graphite/toughened resin composites

NASA Technical Reports Server (NTRS)

Smith, Donald L.; Dow, Marvin B.

1991-01-01

Results are presented from an experimental evaluation of IM7/977-2, IM7/F655, and T800/F3900. Data presented include ply-level (unidirectional laminate) strength and moduli, unnotched and notched (open hole) tension and compression properties of quasi-isotropic laminates, and compression-after-impact strengths. These data are compared with properties of other toughened (IM7/8551-7 and IM6/18081) and brittle (T300/5208) graphite-epoxy materials. The IM7/977-2, IM7/F655, and T800/F3900 materials are substantially stronger and more damage tolerant than widely used first generation composite materials such as T300/5208. The T800/F3900 outperforms IM7/977-2 and IM7/F655 materials in tolerance to projectile impacts. Compression-after-impact strengths were found to be dependent on impactor velocity for a given impact energy. The open hole compression properties of all three materials are degraded by the combination of heat and moisture.

Fused slurry silicide coatings for columbium alloys reentry heat shields. Volume 1: Evaluation analysis

NASA Technical Reports Server (NTRS)

Fitzgerald, B.

1973-01-01

The R-512E (Si-20Cr-20Fe) fused slurry silicide coating process was optimized to coat full size (20in x 20in) single face rib and corrugation stiffened panels fabricated from FS-85 columbium alloy for 100 mission space shuttle heat shield applications. Structural life under simulated space shuttle lift-off stresses and reentry conditions demonstrated reuse capability well beyond 100 flights for R-512E coated FS-85 columbium heat shield panels. Demonstrated coating damage tolerance showed no immediate structural failure on exposure. The FS-85 columbium alloy was selected from five candidate alloys (Cb-752, C-129Y, WC-3015, B-66 and FS-85) based on the evaluation tests which have designed to determine: (1) change in material properties due to coating and reuse; (2) alloy tolerance to coating damage; (3) coating emittance characteristics under reuse conditions; and (4) new coating chemistries for improved coating life.

Tolerance to insect defoliation: biocenotic aspects

Treesearch

Andrey A. Pleshanov; Victor I. Voronin; Elena S. Khlimankova; Valentina I. Epova

1991-01-01

Woody plant resistance to insect damage is of great importance in forest protection, and tree tolerance is an important element of this resistance. The compensating mechanisms responsible for tolerance are nonspecific as a rule and develop after damage has been caused by phytophagous animals or other unfavorable effects. Beyond that, plant tolerance depends on duration...

Damage tolerance in filament-wound graphite/epoxy pressure vessels

NASA Technical Reports Server (NTRS)

Simon, William E.; Ngueyen, Vinh D.; Chenna, Ravi K.

1995-01-01

Graphite/epoxy composites are extensively used in the aerospace and sporting goods industries due to their superior engineering properties compared to those of metals. However, graphite/epoxy is extremely susceptible to impact damage which can cause considerable and sometimes undetected reduction in strength. An inelastic impact model was developed to predict damage due to low-velocity impact. A transient dynamic finite element formulation was used in conjunction with the 3D Tsai-Wu failure criterion to determine and incorporate failure in the materials during impact. Material degradation can be adjusted from no degradation to partial degradation to full degradation. The developed software is based on an object-oriented implementation framework called Extensible Implementation Framework for Finite Elements (EIFFE).

Influence of Fibre Architecture on Impact Damage Tolerance in 3D Woven Composites

NASA Astrophysics Data System (ADS)

Potluri, P.; Hogg, P.; Arshad, M.; Jetavat, D.; Jamshidi, P.

2012-10-01

3D woven composites, due to the presence of through-thickness fibre-bridging, have the potential to improve damage tolerance and at the same time to reduce the manufacturing costs. However, ability to withstand damage depends on weave topology as well as geometry of individual tows. There is an extensive literature on damage tolerance of 2D prepreg laminates but limited work is reported on the damage tolerance of 3D weaves. In view of the recent interest in 3D woven composites from aerospace as well as non-aerospace sectors, this paper aims to provide an understanding of the impact damage resistance as well as damage tolerance of 3D woven composites. Four different 3D woven architectures, orthogonal, angle interlocked, layer-to-layer and modified layer-to-layer structures, have been produced under identical weaving conditions. Two additional structures, Unidirectional (UD) cross-ply and 2D plain weave, have been developed for comparison with 3D weaves. All the four 3D woven laminates have similar order of magnitude of damage area and damage width, but significantly lower than UD and 2D woven laminates. Damage Resistance, calculated as impact energy per unit damage area, has been shown to be significantly higher for 3D woven laminates. Rate of change of CAI strength with impact energy appears to be similar for all four 3D woven laminates as well as UD laminate; 2D woven laminate has higher rate of degradation with respect to impact energy. Undamaged compression strength has been shown to be a function of average tow waviness angle. Additionally, 3D weaves exhibit a critical damage size; below this size there is no appreciable reduction in compression strength. 3D woven laminates have also exhibited a degree of plasticity during compression whereas UD laminates fail instantly. The experimental work reported in this paper forms a foundation for systematic development of computational models for 3D woven architectures for damage tolerance.

Advanced design for lightweight structures: Review and prospects

NASA Astrophysics Data System (ADS)

Braga, Daniel F. O.; Tavares, S. M. O.; da Silva, Lucas F. M.; Moreira, P. M. G. P.; de Castro, Paulo M. S. T.

2014-08-01

Current demand for fuel efficient aircraft has been pushing the aeronautical sector to develop ever more lightweight designs while keeping safe operation and required structural strength. Along with light-weighting, new structural design concepts have also been established in order to maintain the aircraft in service for longer periods of time, with high reliability levels. All these innovations and requirements have led to deeply optimized aeronautical structures contributing to more sustainable air transport. This article reviews the major design philosophies which have been employed in aircraft structures, including safe-life, fail-safe and damage tolerance taking into account their impact on the structural design. A brief historical review is performed in order to analyse what led to the development of each philosophy. Material properties are related to each of the design philosophies. Damage tolerant design has emerged as the main structural design philosophy in aeronautics, requiring deep knowledge on materials fatigue and corrosion strength, as well as potential failure modes and non-destructive inspection techniques, particularly minimum detectable defect and scan times. A discussion on the implementation of structural health monitoring and self-healing structures within the current panorama of structures designed according to the damage tolerant philosophy is presented. This discussion is aided by a review of research on these two subjects. These two concepts show potential for further improving safety and durability of aircraft structures.

Resin transfer molding for advanced composite primary wing and fuselage structures

NASA Technical Reports Server (NTRS)

Markus, Alan

1992-01-01

The stitching and resin transfer molding (RTM) processes developed at Douglas Aircraft Co. are successfully demonstrating significant cost reductions with good damage tolerance properties. These attributes were identified as critical to application of advanced composite materials to commercial aircraft primary structures. The RTM/stitching developments, cost analyses, and test results are discussed of the NASA Advanced Composites Technology program.

An Evaluation of the Applicability of Damage Tolerance to Dynamic Systems

NASA Technical Reports Server (NTRS)

Forth, Scott C.; Le, Dy; Turnberg, Jay

2005-01-01

The Federal Aviation Administration, the National Aeronautics and Space Administration and the aircraft industry have teamed together to develop methods and guidance for the safe life-cycle management of dynamic systems. Based on the success of the United States Air Force damage tolerance initiative for airframe structure, a crack growth based damage tolerance approach is being examined for implementation into the design and management of dynamic systems. However, dynamic systems accumulate millions of vibratory cycles per flight hour, more than 12,000 times faster than an airframe system. If a detectable crack develops in a dynamic system, the time to failure is extremely short, less than 100 flight hours in most cases, leaving little room for error in the material characterization, life cycle analysis, nondestructive inspection and maintenance processes. In this paper, the authors review the damage tolerant design process focusing on uncertainties that affect dynamic systems and evaluate the applicability of damage tolerance on dynamic systems.

Enhancing radiation tolerance by controlling defect mobility and migration pathways in multicomponent single-phase alloys

NASA Astrophysics Data System (ADS)

Lu, Chenyang; Niu, Liangliang; Chen, Nanjun; Jin, Ke; Yang, Taini; Xiu, Pengyuan; Zhang, Yanwen; Gao, Fei; Bei, Hongbin; Shi, Shi; He, Mo-Rigen; Robertson, Ian M.; Weber, William J.; Wang, Lumin

2016-12-01

A grand challenge in material science is to understand the correlation between intrinsic properties and defect dynamics. Radiation tolerant materials are in great demand for safe operation and advancement of nuclear and aerospace systems. Unlike traditional approaches that rely on microstructural and nanoscale features to mitigate radiation damage, this study demonstrates enhancement of radiation tolerance with the suppression of void formation by two orders magnitude at elevated temperatures in equiatomic single-phase concentrated solid solution alloys, and more importantly, reveals its controlling mechanism through a detailed analysis of the depth distribution of defect clusters and an atomistic computer simulation. The enhanced swelling resistance is attributed to the tailored interstitial defect cluster motion in the alloys from a long-range one-dimensional mode to a short-range three-dimensional mode, which leads to enhanced point defect recombination. The results suggest design criteria for next generation radiation tolerant structural alloys.

Tolerance to deer herbivory and resistance to insect herbivores in the common evening primrose (Oenothera biennis).

PubMed

Puentes, A; Johnson, M T J

2016-01-01

The evolution of plant defence in response to herbivory will depend on the fitness effects of damage, availability of genetic variation and potential ecological and genetic constraints on defence. Here, we examine the potential for evolution of tolerance to deer herbivory in Oenothera biennis while simultaneously considering resistance to natural insect herbivores. We examined (i) the effects of deer damage on fitness, (ii) the presence of genetic variation in tolerance and resistance, (iii) selection on tolerance, (iv) genetic correlations with resistance that could constrain evolution of tolerance and (v) plant traits that might predict defence. In a field experiment, we simulated deer damage occurring early and late in the season, recorded arthropod abundances, flowering phenology and measured growth rate and lifetime reproduction. Our study showed that deer herbivory has a negative effect on fitness, with effects being more pronounced for late-season damage. Selection acted to increase tolerance to deer damage, yet there was low and nonsignificant genetic variation in this trait. In contrast, there was substantial genetic variation in resistance to insect herbivores. Resistance was genetically uncorrelated with tolerance, whereas positive genetic correlations in resistance to insect herbivores suggest there exists diffuse selection on resistance traits. In addition, growth rate and flowering time did not predict variation in tolerance, but flowering phenology was genetically correlated with resistance. Our results suggest that deer damage has the potential to exert selection because browsing reduces plant fitness, but limited standing genetic variation in tolerance is expected to constrain adaptive evolution in O. biennis. © 2015 European Society For Evolutionary Biology. Journal of Evolutionary Biology © 2015 European Society For Evolutionary Biology.

Data fusion of multi-scale representations for structural damage detection

NASA Astrophysics Data System (ADS)

Guo, Tian; Xu, Zili

2018-01-01

Despite extensive researches into structural health monitoring (SHM) in the past decades, there are few methods that can detect multiple slight damage in noisy environments. Here, we introduce a new hybrid method that utilizes multi-scale space theory and data fusion approach for multiple damage detection in beams and plates. A cascade filtering approach provides multi-scale space for noisy mode shapes and filters the fluctuations caused by measurement noise. In multi-scale space, a series of amplification and data fusion algorithms are utilized to search the damage features across all possible scales. We verify the effectiveness of the method by numerical simulation using damaged beams and plates with various types of boundary conditions. Monte Carlo simulations are conducted to illustrate the effectiveness and noise immunity of the proposed method. The applicability is further validated via laboratory cases studies focusing on different damage scenarios. Both results demonstrate that the proposed method has a superior noise tolerant ability, as well as damage sensitivity, without knowing material properties or boundary conditions.

3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX): toxicological properties and risk assessment in drinking water.

PubMed

Hirose, A; Nishikawa, A; Kinae, N; Hasegawa, R

1999-01-01

MX (3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone), one of the byproducts formed during the chlorine disinfection process of drinking water, shows strong mutagenic activity for Salmonella strains in the Ames test. In several countries, the contribution of MX to the total mutagenicity of drinking water is estimated to range from 7% to 67%. To assess the risk of MX for human health, we summarized the toxicological properties of MX and estimated the tolerable daily intake (TDI) or tolerable concentration in drinking water. MX is genotoxic in cultured mammalian cells and causes in vivo DNA damage in several tissues. MX is carcinogenic for rodents in addition to possessing skin and gastric promotion activities. From these toxicological profiles of MX, we estimated the virtual safety dose (VSD) for genotoxic action as 5 ng/kg/d and the TDI for non-genotoxic action of MX as 40 ng/kg/d. We assumed a tolerable MX concentration of 150 ng/L in drinking water. Because of the uncertainty about human genotoxicity, however, and the lack of information on reproductive or developmental toxicity, the estimated tolerable dose level may be provisional.

The impact behaviour of silk cocoons.

PubMed

Chen, Fujia; Hesselberg, Thomas; Porter, David; Vollrath, Fritz

2013-07-15

Silk cocoons, constructed by silkmoths (Lepidoptera), are protective structural composites. Some cocoons appear to have evolved towards structural and material optimisation in order to sustain impact strikes from predators and hinder parasite ingress. This study investigates the protective properties of silk cocoons with different morphologies by evaluating their impact resistance and damage tolerance. Finite element analysis was used to analyse empirical observations of the quasi-static impact response of the silk cocoons, and to evaluate the separate benefits of the structures and materials through the deformation and damage mechanism. We use design principles from composite engineering in order to understand the structure-property-function relationship of silkworm cocoons. Understanding the highly evolved survival strategies of the organisms building natural cocoons will hopefully lead to inspiration that in turn could lead to improved composite design.

Pre-damage biomass allocation and not invasiveness predicts tolerance to damage in seedlings of woody species in Hawaii.

PubMed

Lurie, Matthew H; Barton, Kasey E; Daehler, Curtis C

2017-12-01

Plant-herbivore interactions have been predicted to play a fundamental role in plant invasions, although support for this assertion from previous research is mixed. While plants may escape from specialist herbivores in their introduced ranges, herbivory from generalists is common. Tolerance traits may allow non-native plants to mitigate the negative consequences of generalist herbivory that they cannot avoid in their introduced range. Here we address whether tolerance to herbivory, quantified as survival and compensatory growth, is associated with plant invasion success in Hawaii and investigate traits that may enhance tolerance in seedlings, the life stage most susceptible to herbivory. In a greenhouse experiment, we measured seedling tolerance to simulated herbivory through mechanical damage (50% leaf removal) of 16 non-native woody plant species differing in invasion status (invasive vs. non-invasive). Seedlings were grown for 2 weeks following damage and analyzed for biomass to determine whether damaged plants could fully compensate for the lost leaf tissue. Over 99% of all seedlings survived defoliation. Although species varied significantly in their levels of compensation, there was no consistent difference between invasive and non-invasive species. Seedlings of 11 species undercompensated and remained substantially smaller than control seedlings 2 weeks after damage; four species were close to compensating, while one species overcompensated. Across species, compensation was positively associated with an increased investment in potential storage reserves, specifically cotyledons and roots, suggesting that these organs provide resources that help seedlings re-grow following damage. Our results add to a growing consensus that pre-damage growth patterns determine tolerance to damage, even in young seedlings which have relatively low biomass. The lack of higher tolerance in highly invasive species may suggest that invaders overcome herbivory barriers to invasion in other ways, such as resistance traits, or that herbivory does not play an important role in the seedling invasion dynamics of these woody species in Hawaii. © 2017 by the Ecological Society of America.

ACEE composite structures technology

NASA Technical Reports Server (NTRS)

Quinlivan, John T.; Wilson, Robert D.; Smith, Peter J.; Johnson, Ronald W.

1984-01-01

Toppics addressed include: advanced composites on Boeing commercial aircraft; composite wing durability; damage tolerance technology development; heavily loaded wing panel design; and pressure containment and damage tolerance in fuselages.

Applications of a damage tolerance analysis methodology in aircraft design and production

NASA Technical Reports Server (NTRS)

Woodward, M. R.; Owens, S. D.; Law, G. E.; Mignery, L. A.

1992-01-01

Objectives of customer mandated aircraft structural integrity initiatives in design are to guide material selection, to incorporate fracture resistant concepts in the design, to utilize damage tolerance based allowables and planned inspection procedures necessary to enhance the safety and reliability of manned flight vehicles. However, validated fracture analysis tools for composite structures are needed to accomplish these objectives in a timely and economical manner. This paper briefly describes the development, validation, and application of a damage tolerance methodology for composite airframe structures. A closed-form analysis code, entitled SUBLAM was developed to predict the critical biaxial strain state necessary to cause sublaminate buckling-induced delamination extension in an impact damaged composite laminate. An embedded elliptical delamination separating a thin sublaminate from a thick parent laminate is modelled. Predicted failure strains were correlated against a variety of experimental data that included results from compression after impact coupon and element tests. An integrated analysis package was developed to predict damage tolerance based margin-of-safety (MS) using NASTRAN generated loads and element information. Damage tolerance aspects of new concepts are quickly and cost-effectively determined without the need for excessive testing.

Thermal conductivity measurements via time-domain thermoreflectance for the characterization of radiation induced damage

DOE PAGES

Cheaito, Ramez; Gorham, Caroline S.; Carnegie Mellon Univ., Pittsburgh, PA; ...

2015-05-01

The progressive build up of displacement damage and fission products inside different systems and components of a nuclear reactor can lead to significant defect formation, degradation, and damage of the constituent materials. This structural modification can highly influence the thermal transport mechanisms and various mechanical properties of solids. In this paper we demonstrate the use of time-domain thermoreflectance (TDTR), a non-destructive method capable of measuring the thermal transport in material systems from nano to bulk scales, to study the effect of radiation damage and the subsequent changes in the thermal properties of materials. We use TDTR to show that displacementmore » damage from ion irradiation can significantly reduce the thermal conductivity of Optimized ZIRLO, a material used as fuel cladding in several current nuclear reactors. We find that the thermal conductivity of copper-niobium nanostructured multilayers does not change with helium ion irradiation doses of up to 10 15 cm -2 and ion energy of 200 keV suggesting that these structures can be used and radiation tolerant materials in nuclear reactors. We compare the effect of ion doses and ion beam energies on the measured thermal conductivity of bulk silicon. Results demonstrate that TDTR thermal measurements can be used to quantify depth dependent damage.« less

Micromechanics of fatigue in woven and stitched composites

NASA Technical Reports Server (NTRS)

Cox, B. N.; Carter, W. C.; Dadkhah, M. S.; Morris, W. L.

1994-01-01

The goals of this research program were to: (1) determine how microstructural factors, especially the architecture of reinforcing fibers, control stiffness, strength, and fatigue life in 3D woven composites; (2) identify mechanisms of failure; (3) model composite stiffness; (4) model notched and unnotched strength; and (5) model fatigue life. We have examined a total of eleven different angle and orthogonal interlock woven composites. Extensive testing has revealed that these 3D woven composites possess an extraordinary combination of strength, damage tolerance, and notch insensitivity in compression and tension and in monotonic and cyclic loading. In many important regards, 3D woven composites far outstrip conventional 2D laminates or stitched laminates. Detailed microscopic analysis of damage has led to a comprehensive picture of the essential mechanisms of failure and how they are related to the reinforcement geometry. The critical characteristics of the weave architecture that promote favorable properties have been identified. Key parameters are tow size and the distributions in space and strength of geometrical flaws. The geometrical flaws should be regarded as controllable characteristics of the weave in design and manufacture. In addressing our goals, the simplest possible models of properties were always sought, in a blend of old and new modeling concepts. Nevertheless, certain properties, especially regarding damage tolerance, ultimate failure, and the detailed effects of weave architecture, require computationally intensive stochastic modeling. We have developed a new model, the 'binary model,' to carry out such tasks in the most efficient manner and with faithful representation of crucial mechanisms. This is the final report for contract NAS1-18840. It covers all work from April 1989 up to the conclusion of the program in January 1993.

Sandwich Structure Risk Reduction in Support of the Payload Adapter Fitting

NASA Technical Reports Server (NTRS)

Nettles, A. T.; Jackson, J. R.; Guin, W. E.

2018-01-01

Reducing risk for utilizing honeycomb sandwich structure for the Space Launch System payload adapter fitting includes determining what parameters need to be tested for damage tolerance to ensure a safe structure. Specimen size and boundary conditions are the most practical parameters to use in damage tolerance inspection. The effect of impact over core splices and foreign object debris between the facesheet and core is assessed. Effects of enhanced damage tolerance by applying an outer layer of carbon fiber woven cloth is examined. A simple repair technique for barely visible impact damage that restores all compression strength is presented.

Antioxidant defense parameters as predictive biomarkers for fermentative capacity of active dried wine yeast.

PubMed

Gamero-Sandemetrio, Esther; Gómez-Pastor, Rocío; Matallana, Emilia

2014-08-01

The production of active dried yeast (ADY) is a common practice in industry for the maintenance of yeast starters and as a means of long term storage. The process, however, causes multiple cell injuries, with oxidative damage being one of the most important stresses. Consequentially, dehydration tolerance is a highly appreciated property in yeast for ADY production. In this study we analyzed the cellular redox environment in three Saccharomyces cerevisiae wine strains, which show markedly different fermentative capacities after dehydration. To measure/quantify the effect of dehydration on the S. cerevisiae strains, we used: (i) fluorescent probes; (ii) antioxidant enzyme activities; (ii) intracellular damage; (iii) antioxidant metabolites; and (iv) gene expression, to select a minimal set of biochemical parameters capable of predicting desiccation tolerance in wine yeasts. Our results show that naturally enhanced antioxidant defenses prevent oxidative damage after wine yeast biomass dehydration and improve fermentative capacity. Based on these results we chose four easily assayable parameters/biomarkers for the selection of industrial yeast strains of interest for ADY production: trehalose and glutathione levels, and glutathione reductase and catalase enzymatic activities. Yeast strains selected in accordance with this process display high levels of trehalose, low levels of oxidized glutathione, a high induction of glutathione reductase activity, as well as a high basal level and sufficient induction of catalase activity, which are properties inherent in superior ADY strains. Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Effects of intra- and inter-laminar resin content on the mechanical properties of toughened composite materials

NASA Technical Reports Server (NTRS)

Grande, Dodd H.; Ilcewicz, Larry B.; Avery, William B.; Bascom, Willard D.

1991-01-01

Composite materials having multiphase toughened matrix systems and laminate architectures characterized by resin-rich interlaminar layers (RIL) have been the subject of much recent attention. Such materials are likely to find applications in thick compressively loaded structures such as the keel area of commercial aircraft fuselages. The effects of resin content and its interlaminar and intralaminar distribution on mechanical properties were investigated with test and analysis of two carbon-epoxy systems. The RIL was found to reduce the in situ strengthening effect for matrix cracking in laminates. Mode 2 fracture toughness was found to increase with increasing RIL thickness over the range investigated, and Mode 1 interlaminar toughness was negligibly affected. Compressive failure strains were found to increase with increasing resin content for specimens having no damage, holes, and impact damage. Analytical tools for predicting matrix cracking of off-axis plies and damage tolerance in compression after impact (CAI) were successfully applied to materials with RIL.

Effect of Translaminar Reinforcements and Hybridization on Damage Resistance and Tolerance of Composite Laminates

DTIC Science & Technology

2012-01-01

REINFORCEMENTS AND HYBRIDIZATION ON DAMAGE RESISTANCE AND TOLERANCE OF COMPOSITE LAMINATES It was shown that the damage resistance and tolerance of... laminated composites can be enhanced by the employment of translaminar reinforcements (TLR) such as stitching, z-pinning and 3D weaving and also by hybrid...Park, NC 27709-2211 Composite Laminates Resistance REPORT DOCUMENTATION PAGE 11. SPONSOR/MONITOR’S REPORT NUMBER(S) 10. SPONSOR/MONITOR’S ACRONYM

The US Navy’s Helicopter Integrated Diagnostics System (HIDS) Program: Power Drive Train Crack Detection Diagnostics and Prognostics Life Usage Monitoring and Damage Tolerance; Techniques, Methodologies, and Experiences

DTIC Science & Technology

2000-02-01

HIDS] Program: Power Drive Train Crack Detection Diagnostics and Prognostics ife Usage Monitoring and Damage Tolerance; Techniques, Methodologies, and...and Prognostics , Life Usage Monitoring , and Damage Tolerance; Techniques, Methodologies, and Experiences Andrew Hess Harrison Chin William Hardman...continuing program and deployed engine monitoring systems in fixed to evaluate helicopter diagnostic, prognostic , and wing aircraft, notably on the A

14 CFR 29.573 - Damage Tolerance and Fatigue Evaluation of Composite Rotorcraft Structures.

Code of Federal Regulations, 2012 CFR

2012-01-01

... Composite Rotorcraft Structures. 29.573 Section 29.573 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... Structures. (a) Each applicant must evaluate the composite rotorcraft structure under the damage tolerance..., types, and sizes of damage, considering fatigue, environmental effects, intrinsic and discrete flaws...

14 CFR 27.573 - Damage Tolerance and Fatigue Evaluation of Composite Rotorcraft Structures.

Code of Federal Regulations, 2012 CFR

2012-01-01

... Composite Rotorcraft Structures. 27.573 Section 27.573 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... Structures. (a) Each applicant must evaluate the composite rotorcraft structure under the damage tolerance..., types, and sizes of damage, considering fatigue, environmental effects, intrinsic and discrete flaws...

14 CFR 27.573 - Damage Tolerance and Fatigue Evaluation of Composite Rotorcraft Structures.

Code of Federal Regulations, 2013 CFR

2013-01-01

... Composite Rotorcraft Structures. 27.573 Section 27.573 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... Structures. (a) Each applicant must evaluate the composite rotorcraft structure under the damage tolerance..., types, and sizes of damage, considering fatigue, environmental effects, intrinsic and discrete flaws...

14 CFR 29.573 - Damage Tolerance and Fatigue Evaluation of Composite Rotorcraft Structures.

Code of Federal Regulations, 2013 CFR

2013-01-01

... Composite Rotorcraft Structures. 29.573 Section 29.573 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... Structures. (a) Each applicant must evaluate the composite rotorcraft structure under the damage tolerance..., types, and sizes of damage, considering fatigue, environmental effects, intrinsic and discrete flaws...

14 CFR 27.573 - Damage Tolerance and Fatigue Evaluation of Composite Rotorcraft Structures.

Code of Federal Regulations, 2014 CFR

2014-01-01

... Composite Rotorcraft Structures. 27.573 Section 27.573 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... Structures. (a) Each applicant must evaluate the composite rotorcraft structure under the damage tolerance..., types, and sizes of damage, considering fatigue, environmental effects, intrinsic and discrete flaws...

14 CFR 29.573 - Damage Tolerance and Fatigue Evaluation of Composite Rotorcraft Structures.

Code of Federal Regulations, 2014 CFR

2014-01-01

... Composite Rotorcraft Structures. 29.573 Section 29.573 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... Structures. (a) Each applicant must evaluate the composite rotorcraft structure under the damage tolerance..., types, and sizes of damage, considering fatigue, environmental effects, intrinsic and discrete flaws...

7 CFR 51.2954 - Tolerances for grade defects.

Code of Federal Regulations, 2011 CFR

2011-01-01

... damaged by mold or insects or seriously damaged by other means, of which not more than 5/6 or 5 pct may be damaged by insects, but no part of any tolerance shall be allowed for walnuts containing live insects No... adhering hulls 15 pct total, by count, including not more than 8 pct which are damaged by mold or insects...

A Novel Approach to Rotorcraft Damage Tolerance

NASA Technical Reports Server (NTRS)

Forth, Scott C.; Everett, Richard A.; Newman, John A.

2002-01-01

Damage-tolerance methodology is positioned to replace safe-life methodologies for designing rotorcraft structures. The argument for implementing a damage-tolerance method comes from the fundamental fact that rotorcraft structures typically fail by fatigue cracking. Therefore, if technology permits prediction of fatigue-crack growth in structures, a damage-tolerance method should deliver the most accurate prediction of component life. Implementing damage-tolerance (DT) into high-cycle-fatigue (HCF) components will require a shift from traditional DT methods that rely on detecting an initial flaw with nondestructive inspection (NDI) methods. The rapid accumulation of cycles in a HCF component will result in a design based on a traditional DT method that is either impractical because of frequent inspections, or because the design will be too heavy to operate efficiently. Furthermore, once a HCF component develops a detectable propagating crack, the remaining fatigue life is short, sometimes less than one flight hour, which does not leave sufficient time for inspection. Therefore, designing a HCF component will require basing the life analysis on an initial flaw that is undetectable with current NDI technology.

Seed tolerance to predation: Evidence from the toxic seeds of the buckeye tree (Aesculus californica; Sapindaceae).

PubMed

Mendoza, Eduardo; Dirzo, Rodolfo

2009-07-01

Tolerance, the capacity of plants to withstand attack by animals, as opposed to resistance, has been poorly examined in the context of seed predation. We investigated the role that the seed mass of the large-seeded endemic tree Aesculus californica plays as a tolerance trait to rodent attack by comparing, under greenhouse conditions, patterns of germination, and subsequent seedling growth, of seeds with a wide range of natural damage. Germination percentage was reduced by 50% and time to germination by 64% in attacked compared to intact seeds, and germination probability was negatively correlated with damage. Seedlings that emerged from intact seeds were taller and bore more leaves than those from damaged seeds. This species' large seed mass favors tolerance to damage because heavily damaged seeds are able to germinate and produce seedlings. This finding is significant given that seeds of this species are known to contain chemical compounds toxic to vertebrates, a resistance trait. We posit that this combination of tolerance and resistance traits might be a particularly effective antipredation strategy when seeds are exposed to a variety of vertebrate predators.

A damage tolerance comparison of IM7/8551 and IM8G/8553 carbon/epoxy composites

NASA Technical Reports Server (NTRS)

Lance, D. G.; Nettles, A. T.

1991-01-01

A damage tolerance study of two new toughened carbon fiber/epoxy resin systems was undertaken as a continuation of ongoing work into screening new opposites for resistance to foreign object impact. This report is intended to be a supplement to NASA TP 3029 in which four new fiber/resin systems were tested for damage tolerance. Instrumented drop weight impact testing was used to inflict damage to 16-ply quasi-isotropic specimens. Instrumented output data and cross-sectional examinations of the damage zone were utilized to quantify the damage. It was found that the two fiber/resin systems tested in this study were much more impact resistant than an untoughened composite such as T300/934, but were not as impact resistant as other materials previously studied.

Probabilistic Assessment of Fracture Progression in Composite Structures

NASA Technical Reports Server (NTRS)

Chamis, Christos C.; Minnetyan, Levon; Mauget, Bertrand; Huang, Dade; Addi, Frank

1999-01-01

This report describes methods and corresponding computer codes that are used to evaluate progressive damage and fracture and to perform probabilistic assessment in built-up composite structures. Structural response is assessed probabilistically, during progressive fracture. The effects of design variable uncertainties on structural fracture progression are quantified. The fast probability integrator (FPI) is used to assess the response scatter in the composite structure at damage initiation. The sensitivity of the damage response to design variables is computed. The methods are general purpose and are applicable to stitched and unstitched composites in all types of structures and fracture processes starting from damage initiation to unstable propagation and to global structure collapse. The methods are demonstrated for a polymer matrix composite stiffened panel subjected to pressure. The results indicated that composite constituent properties, fabrication parameters, and respective uncertainties have a significant effect on structural durability and reliability. Design implications with regard to damage progression, damage tolerance, and reliability of composite structures are examined.

An Experimental Investigation of Damage Resistances and Damage Tolerance of Composite Materials

NASA Technical Reports Server (NTRS)

Prabhakaran, R.

2003-01-01

The project included three lines of investigation, aimed at a better understanding of the damage resistance and damage tolerance of pultruded composites. The three lines of investigation were: (i) measurement of permanent dent depth after transverse indentation at different load levels, and correlation with other damage parameters such as damage area (from x-radiography) and back surface crack length, (ii) estimation of point stress and average stress characteristic dimensions corresponding to measured damage parameters, and (iii) an attempt to measure the damage area by a reflection photoelastic technique. All the three lines of investigation were pursued.

Hierarchical flexural strength of enamel: transition from brittle to damage-tolerant behaviour

PubMed Central

Bechtle, Sabine; Özcoban, Hüseyin; Lilleodden, Erica T.; Huber, Norbert; Schreyer, Andreas; Swain, Michael V.; Schneider, Gerold A.

2012-01-01

Hard, biological materials are generally hierarchically structured from the nano- to the macro-scale in a somewhat self-similar manner consisting of mineral units surrounded by a soft protein shell. Considerable efforts are underway to mimic such materials because of their structurally optimized mechanical functionality of being hard and stiff as well as damage-tolerant. However, it is unclear how different hierarchical levels interact to achieve this performance. In this study, we consider dental enamel as a representative, biological hierarchical structure and determine its flexural strength and elastic modulus at three levels of hierarchy using focused ion beam (FIB) prepared cantilevers of micrometre size. The results are compared and analysed using a theoretical model proposed by Jäger and Fratzl and developed by Gao and co-workers. Both properties decrease with increasing hierarchical dimension along with a switch in mechanical behaviour from linear-elastic to elastic-inelastic. We found Gao's model matched the results very well. PMID:22031729

Damage Tolerance Assessment Branch

NASA Technical Reports Server (NTRS)

Walker, James L.

2013-01-01

The Damage Tolerance Assessment Branch evaluates the ability of a structure to perform reliably throughout its service life in the presence of a defect, crack, or other form of damage. Such assessment is fundamental to the use of structural materials and requires an integral blend of materials engineering, fracture testing and analysis, and nondestructive evaluation. The vision of the Branch is to increase the safety of manned space flight by improving the fracture control and the associated nondestructive evaluation processes through development and application of standards, guidelines, advanced test and analytical methods. The Branch also strives to assist and solve non-aerospace related NDE and damage tolerance problems, providing consultation, prototyping and inspection services.

Damage tolerant functionally graded materials for advanced wear and friction applications

NASA Astrophysics Data System (ADS)

Prchlik, Lubos

The research work presented in this dissertation focused on processing effects, microstructure development, characterization and performance evaluation of composite and graded coatings used for friction and wear control. The following issues were addressed. (1) Definition of prerequisites for a successful composite and graded coating formation by means of thermal spraying. (2) Improvement of characterization methods available for homogenous thermally sprayed coating and their extension to composite and graded materials. (3) Development of novel characterization methods specifically for FGMs, with a focus on through thickness property measurement by indentation and in-situ curvature techniques. (4) Design of composite materials with improved properties compared to homogenous coatings. (5) Fabrication and performance assessment of FGM with improved wear and impact damage properties. Materials. The materials studied included several material systems relevant to low friction and contact damage tolerant applications: MO-Mo2C, WC-Co cermets as materials commonly used sliding components of industrial machinery and NiCrAlY/8%-Yttria Partially Stabilized Zirconia composites as a potential solution for abradable sections of gas turbines and aircraft engines. In addition, uniform coatings such as molybdenum and Ni5%Al alloy were evaluated as model system to assess the influence of microstructure variation onto the mechanical property and wear response. Methods. The contact response of the materials was investigated through several techniques. These included methods evaluating the relevant intrinsic coating properties such as elastic modulus, residual stress, fracture toughness, scratch resistance and tests measuring the abrasion and friction-sliding behavior. Dry-sand and wet two-body abrasion testing was performed in addition to traditional ball on disc sliding tests. Among all characterization techniques the spherical indentation deserved most attention and enabled to measure elastic-plastic properties of uniform and graded structures. In-situ curvature method used for residual stress and elastic modulus measurement was extended from uniform coatings to coatings with compositional/property gradients. Properties of composite and graded materials were measured using the inverse analysis. Conclusions. The specifics of the elastic-plastic response for thermally sprayed coatings were demonstrated. These included the strain dependence of elastic modulus and damage accumulation related to unloading/reloading loop formation. The measurement of elastic-plastic characteristics of composite coatings revealed the mixing and bonding mechanisms unique for thermally sprayed materials. Microstructural and compositional factors governing the frictional vs. abrasion response of carbide-metallic composite coatings were described. The measurement of abrasion resistance and friction sliding properties demonstrated that grading of cermet and ceramic coatings by adding moderate amount of metallic alloys can enhance elastic-properties radically and have a beneficial effect onto the coating performance.

Application of damage tolerance methodology in certification of the Piaggio P-180 Avanti

NASA Technical Reports Server (NTRS)

Johnson, Jerry

1992-01-01

The Piaggio P-180 Avanti, a twin pusher-prop engine nine-passenger business aircraft was certified in 1990, to the requirements of FAR Part 23 and Associated Special Conditions for Composite Structure. Certification included the application of a damage tolerant methodology to the design of the composite forward wing and empennage (vertical fin, horizontal stabilizer, tailcone, and rudder) structure. This methodology included an extensive analytical evaluation coupled with sub-component and full-scale testing of the structure. The work from the Damage Tolerance Analysis Assessment was incorporated into the full-scale testing. Damage representing hazards such as dropped tools, ground equipment, handling, and runway debris, was applied to the test articles. Additional substantiation included allowing manufacturing discrepancies to exist unrepaired on the full-scale articles and simulated bondline failures in critical elements. The importance of full-scale testing in the critical environmental conditions and the application of critical damage are addressed. The implication of damage tolerance on static and fatigue testing is discussed. Good correlation between finite element solutions and experimental test data was observed.

Low velocity instrumented impact testing of four new damage tolerant carbon/epoxy composite systems

NASA Technical Reports Server (NTRS)

Lance, D. G.; Nettles, A. T.

1990-01-01

Low velocity drop weight instrumented impact testing was utilized to examine the damage resistance of four recently developed carbon fiber/epoxy resin systems. A fifth material, T300/934, for which a large data base exists, was also tested for comparison purposes. A 16-ply quasi-isotropic lay-up configuration was used for all the specimens. Force/absorbed energy-time plots were generated for each impact test. The specimens were cross-sectionally analyzed to record the damage corresponding to each impact energy level. Maximum force of impact versus impact energy plots were constructed to compare the various systems for impact damage resistance. Results show that the four new damage tolerant fiber/resin systems far outclassed the T300/934 material. The most damage tolerant material tested was the IM7/1962 fiber/resin system.

Effects of thermal and moisture cycling on the internal structure of stitched RTM laminates

NASA Technical Reports Server (NTRS)

Walker, Jeff; Roundy, Lance; Goering, Jon

1993-01-01

Conventional aerospace composites are strong and stiff in the directions parallel to the carbon fibers, but they are prone to delaminations and damage in the through-the-thickness directions. Recent research has shown that substantial improvements in damage tolerance are obtained from textile composites with Z-direction reinforcement provided by stitching, weaving, or braiding. Because of the mismatch in thermal and moisture expansion properties of the various material components, there is a potential for microcracks to develop in the resin matrix. These cracks can form to relieve the mechanical stresses that are generated during curing or in-service temperature cycles.

The development of Nb-based advanced intermetallic alloys for structural applications

NASA Astrophysics Data System (ADS)

Subramanian, P. R.; Mendiratta, M. G.; Dimiduk, D. M.

1996-01-01

A new generation of refractory material systems with significant increases in temperature capability is required to meet the demands of future aerospace applications. Such materials require a balance of properties such as low-temperature damage tolerance, high-temperature strength, creep resistance, and superior environmental stability for implementation in advanced aerospace systems. Systems incorporating niobium-based beta alloys and intermetallic compounds have the potential for meeting these requirements.

Mechanical Properties of Steel Encapsulated Metal Matrix Composites

NASA Astrophysics Data System (ADS)

Fudger, Sean; Klier, Eric; Karandikar, Prashant; McWilliams, Brandon; Ni, Chaoying

This research evaluates a coefficient of thermal expansion (CTE) mismatch induced residual compressive stress approach as a means of improving the ductility of metal matrix composites (MMCs). MMCs are frequently incorporated into advanced material systems due to their tailorable material properties. However, they often have insufficient strength and ductility for many structural applications. By combining MMCs with high strength steels in a hybridized, macro composite materials system that exploits the CTE mismatch, materials systems with improved strength, damage tolerance, and structural efficiency can be obtained. Macro hybridized systems consisting of steel encapsulated light metal MMCs were produced with the goal of creating a system which takes advantage of the high strength, modulus, and damage tolerance of steels and high specific stiffness and low density of MMCs while mitigating the high density of steels and the poor ductility of MMCs. Aluminum and magnesium based particulate reinforced MMCs combine many of the desirable characteristic of metals and ceramics, particularly the unique ability to tailor their CTE. This work aims to compare the performance of macro hybridized material systems consisting of aluminum or magnesium MMCs reinforced with Al2O3, SiC, or B4C particles and encapsulated by A36 steel, 304 stainless steel, or cold worked Nitronic® 50 stainless steels.

Damage Tolerance Applied to Design of Mid-Size Aircraft

NASA Astrophysics Data System (ADS)

Chaves, Carlos Eduardo

Most of the mid-size aircraft are certified according to FAA Part 25 requirements, and in order to comply with these requirements the majority of the aircraft structure must be damage tolerant. To assure damage tolerance, despite the overall structural behavior, one should look at the details. There is a great amount of analysis tasks and tests that must be carried out in order to guarantee the aircraft structural integrity. This paper presents an overview of Embraer experience with design and analysis for damage tolerance during the last 30 years. Aspects like DT analysis for metallic and composite structures, selection of appropriate materials, loads, definition of limits of validity and definition of inspection intervals will be addressed along this work. Selected structural tests that have been performed for validation of modeling predictions will be presented. Some aspects to be discussed are related to the design differences between commercial jets, which are usually subjected to high usage conditions, business jets and military aircraft. Further, the application of future technologies, such as structural health monitoring, and also of new materials and manufacturing processes that have been evaluated in order to improve the damage tolerance capability of the aircraft structures will be discussed.

Single-Phase Concentrated Solid-Solution Alloys: Bridging Intrinsic Transport Properties and Irradiation Resistance

DOE PAGES

Jin, Ke; Bei, Hongbin

2018-04-30

Single-phase concentrated solid-solution alloys (SP-CSAs), including high entropy alloys (HEAs), are compositionally complex but structurally simple, and provide a playground of tailoring material properties through modifying their compositional complexity. The recent progress in understanding the compositional effects on the energy and mass transport properties in a series of face-centered-cubic SP-CSAs is the focus of this review. Relatively low electrical and thermal conductivities, as well as small separations between the interstitial and vacancy migration barriers have been generally observed, but largely depend on the alloying constituents. We further discuss the impact of such intrinsic transport properties on their irradiation response; themore » linkage to the delayed damage accumulation, slow defect aggregation, and suppressed irradiation induced swelling and segregation has been presented. We emphasize that the number of alloying elements may not be a critical factor on both transport properties and the defect behaviors under ion irradiations. Furthermore, the recent findings have stimulated novel concepts in the design of new radiation-tolerant materials, but further studies are demanded to enable predictive models that can quantitatively bridge the transport properties to the radiation damage.« less

Single-Phase Concentrated Solid-Solution Alloys: Bridging Intrinsic Transport Properties and Irradiation Resistance

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jin, Ke; Bei, Hongbin

Single-phase concentrated solid-solution alloys (SP-CSAs), including high entropy alloys (HEAs), are compositionally complex but structurally simple, and provide a playground of tailoring material properties through modifying their compositional complexity. The recent progress in understanding the compositional effects on the energy and mass transport properties in a series of face-centered-cubic SP-CSAs is the focus of this review. Relatively low electrical and thermal conductivities, as well as small separations between the interstitial and vacancy migration barriers have been generally observed, but largely depend on the alloying constituents. We further discuss the impact of such intrinsic transport properties on their irradiation response; themore » linkage to the delayed damage accumulation, slow defect aggregation, and suppressed irradiation induced swelling and segregation has been presented. We emphasize that the number of alloying elements may not be a critical factor on both transport properties and the defect behaviors under ion irradiations. Furthermore, the recent findings have stimulated novel concepts in the design of new radiation-tolerant materials, but further studies are demanded to enable predictive models that can quantitatively bridge the transport properties to the radiation damage.« less

Minimizing the regrets of long-term urban floodplain management decisions under deeply uncertain climate change

NASA Astrophysics Data System (ADS)

Hecht, J. S.; Kirshen, P. H.; Vogel, R. M.

2016-12-01

Making long-term floodplain management decisions under uncertain climate change is a major urban planning challenge of the 21stcentury. To support these efforts, we introduce a screening-level optimization model that identifies adaptation portfolios by minimizing the regrets associated with their flood-control and damage costs under different climate change trajectories that are deeply uncertain, i.e. have probabilities that cannot be specified plausibly. This mixed integer program explicitly considers the coupled damage-reduction impacts of different floodwall designs and property-scale investments (first-floor elevation, wet floodproofing of basements, permanent retreat and insurance), recommends implementation schedules, and assesses impacts to stakeholders residing in three types of homes. An application to a stylized municipality illuminates many nonlinear system dynamics stemming from large fixed capital costs, infrastructure design thresholds, and discharge-depth-damage relationships. If stakeholders tolerate mild damage, floodwalls that fully protect a community from large design events are less cost-effective than portfolios featuring both smaller floodwalls and property-scale measures. Potential losses of property tax revenue from permanent retreat motivate municipal property-tax initiatives for adaptation financing. Yet, insurance incentives for first-floor elevation may discourage locally financed floodwalls, in turn making lower-income residents more vulnerable to severe flooding. A budget constraint analysis underscores the benefits of flexible floodwall designs with low incremental expansion costs while near-optimal solutions demonstrate the scheduling flexibility of many property-scale measures. Finally, an equity analysis shows the importance of evaluating the overpayment and under-design regrets of recommended adaptation portfolios for each stakeholder and contrasts them to single-scenario model results.

Damage Mechanisms and Mechanical Properties of High-Strength Multiphase Steels.

PubMed

Heibel, Sebastian; Dettinger, Thomas; Nester, Winfried; Clausmeyer, Till; Tekkaya, A Erman

2018-05-09

The usage of high-strength steels for structural components and reinforcement parts is inevitable for modern car-body manufacture in reaching lightweight design as well as increasing passive safety. Depending on their microstructure these steels show differing damage mechanisms and various mechanical properties which cannot be classified comprehensively via classical uniaxial tensile testing. In this research, damage initiation, evolution and final material failure are characterized for commercially produced complex-phase (CP) and dual-phase (DP) steels in a strength range between 600 and 1000 MPa. Based on these investigations CP steels with their homogeneous microstructure are characterized as damage tolerant and hence less edge-crack sensitive than DP steels. As final fracture occurs after a combination of ductile damage evolution and local shear band localization in ferrite grains at a characteristic thickness strain, this strain measure is introduced as a new parameter for local formability. In terms of global formability DP steels display advantages because of their microstructural composition of soft ferrite matrix including hard martensite particles. Combining true uniform elongation as a measure for global formability with the true thickness strain at fracture for local formability the mechanical material response can be assessed on basis of uniaxial tensile testing incorporating all microstructural characteristics on a macroscopic scale. Based on these findings a new classification scheme for the recently developed high-strength multiphase steels with significantly better formability resulting of complex underlying microstructures is introduced. The scheme overcomes the steel designations using microstructural concepts, which provide no information about design and production properties.

Alumina additions may improve the damage tolerance of soft machined zirconia-based ceramics.

PubMed

Oilo, Marit; Tvinnereim, Helene M; Gjerdet, Nils Roar

2011-01-01

The aim of this study was to evaluate the damage tolerance of different zirconia-based materials. Bars of one hard machined and one soft machined dental zirconia and an experimental 95% zirconia 5% alumina ceramic were subjected to 100,000 stress cycles (n = 10), indented to provoke cracks on the tensile stress side (n = 10), and left untreated as controls (n = 10). The experimental material demonstrated a higher relative damage tolerance, with a 40% reduction compared to 68% for the hard machined zirconia and 84% for the soft machined zirconia.

Safe-life and damage-tolerant design approaches for helicopter structures

NASA Technical Reports Server (NTRS)

Reddick, H. K., Jr.

1983-01-01

The safe-life and damage-tolerant design approaches discussed apply to both metallic and fibrous composite helicopter structures. The application of these design approaches to fibrous composite structures is emphasized. Safe-life and damage-tolerant criteria are applied to all helicopter flight critical components, which are generally categorized as: dynamic components with a main and tail rotor system, which includes blades, hub and rotating controls, and drive train which includes transmission, and main and interconnecting rotor shafts; and the airframe, composed of the fuselage, aerodynamic surfaces, and landing gear.

Enhancing radiation tolerance by controlling defect mobility and migration pathways in multicomponent single-phase alloys

DOE PAGES

Lu, Chenyang; Niu, Liangliang; Chen, Nanjun; ...

2016-12-15

A grand challenge in material science is to understand the correlation between intrinsic properties and defect dynamics. Radiation tolerant materials are in great demand for safe operation and advancement of nuclear and aerospace systems. Unlike traditional approaches that rely on microstructural and nanoscale features to mitigate radiation damage, this study demonstrates enhancement of radiation tolerance with the suppression of void formation by two orders magnitude at elevated temperatures in equiatomic single-phase concentrated solid solution alloys, and more importantly, reveals its controlling mechanism through a detailed analysis of the depth distribution of defect clusters and an atomistic computer simulation. The enhancedmore » swelling resistance is attributed to the tailored interstitial defect cluster motion in the alloys from a long-range one-dimensional mode to a short-range three-dimensional mode, which leads to enhanced point defect recombination. Finally, the results suggest design criteria for next generation radiation tolerant structural alloys.« less

Meta-analysis of attitudes toward damage-causing mammalian wildlife.

PubMed

Kansky, Ruth; Kidd, Martin; Knight, Andrew T

2014-08-01

Many populations of threatened mammals persist outside formally protected areas, and their survival depends on the willingness of communities to coexist with them. An understanding of the attitudes, and specifically the tolerance, of individuals and communities and the factors that determine these is therefore fundamental to designing strategies to alleviate human-wildlife conflict. We conducted a meta-analysis to identify factors that affected attitudes toward 4 groups of terrestrial mammals. Elephants (65%) elicited the most positive attitudes, followed by primates (55%), ungulates (53%), and carnivores (44%). Urban residents presented the most positive attitudes (80%), followed by commercial farmers (51%) and communal farmers (26%). A tolerance to damage index showed that human tolerance of ungulates and primates was proportional to the probability of experiencing damage while elephants elicited tolerance levels higher than anticipated and carnivores elicited tolerance levels lower than anticipated. Contrary to conventional wisdom, experiencing damage was not always the dominant factor determining attitudes. Communal farmers had a lower probability of being positive toward carnivores irrespective of probability of experiencing damage, while commercial farmers and urban residents were more likely to be positive toward carnivores irrespective of damage. Urban residents were more likely to be positive toward ungulates, elephants, and primates when probability of damage was low, but not when it was high. Commercial and communal farmers had a higher probability of being positive toward ungulates, primates, and elephants irrespective of probability of experiencing damage. Taxonomic bias may therefore be important. Identifying the distinct factors explaining these attitudes and the specific contexts in which they operate, inclusive of the species causing damage, will be essential for prioritizing conservation investments. © 2014 The Authors. Conservation Biology published by Wiley Periodicals, Inc., on behalf of the Society for Conservation Biology.

75 FR 16696 - Airworthiness Directives; Airbus Model A330-223, -321, -322, and -323 Airplanes

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-02

... Damage Tolerant--Airworthiness Limitation Item task 712106-01-01 from A330 ALS Part 2, an A330 operator...: During accomplishment of Damage Tolerant--Airworthiness Limitation Item task 712106-01-01 from A330 ALS... Tolerant--Airworthiness Limitation Item task 712106-01-01 from A330 ALS Part 2, an A330 operator found a...

Damage-Tolerance and Fatigue Evaluation of Structure

DOT National Transportation Integrated Search

1997-02-18

This advisory circular (AC) sets forth an acceptable means of compliance : with the provisions of Part 25 of the Federal Aviation Regulations (FAR) dealing with the damage-tolerance and fatigue evaluation requirements of transport category aircraft s...

Impact damage resistance and damage suppression properties of shape memory alloys in hybrid composites—a review

NASA Astrophysics Data System (ADS)

Angioni, S. L.; Meo, M.; Foreman, A.

2011-01-01

Composite materials are known to have a poor resistance to through-the-thickness impact loading. There are various methods for improving their impact damage tolerance, such as fiber toughening, matrix toughening, interface toughening, through-the-thickness reinforcements, and selective interlayers and hybrids. Hybrid composites with improved impact resistance are particularly useful in military and commercial civil applications. Hybridizing composites using shape memory alloys (SMA) is one solution since SMA materials can absorb the energy of the impact through superelastic deformation or recovery stress, reducing the effects of the impact on the composite structure. The SMA material may be embedded in the hybrid composites (SMAHC) in many different forms and also the characteristics of the fiber reinforcements may vary, such as SMA wires in woven laminates or SMA foils in unidirectional laminates, only to cite two examples. We will review the state of the art of SMAHC for the purpose of damage suppression. Both the active and passive damage suppression mechanisms will be considered.

Advanced composite structural concepts and materials technologies for primary aircraft structures: Advanced material concepts

NASA Technical Reports Server (NTRS)

Lau, Kreisler S. Y.; Landis, Abraham L.; Chow, Andrea W.; Hamlin, Richard D.

1993-01-01

To achieve acceptable performance and long-term durability at elevated temperatures (350 to 600 F) for high-speed transport systems, further improvements of the high-performance matrix materials will be necessary to achieve very long-term (60,000-120,000 service hours) retention of mechanical properties and damage tolerance. This report emphasizes isoimide modification as a complementary technique to semi-interpenetrating polymer networks (SIPN's) to achieve greater processibility, better curing dynamics, and possibly enhanced thermo-mechanical properties in composites. A key result is the demonstration of enhanced processibility of isoimide-modified linear and thermo-setting polyimide systems.

Cancer cells recovering from damage exhibit mitochondrial restructuring and increased aerobic glycolysis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Akakura, Shin; Ostrakhovitch, Elena; Sanokawa-Akakura, Reiko

2014-06-13

Highlights: • Some cancer cells recover from severe damage that causes cell death in majority of cells. • Damage-Recovered (DR) cancer cells show reduced mitochondria, mDNA and mitochondrial enzymes. • DR cells show increased aerobic glycolysis, ATP, cell proliferation, and resistance to damage. • DR cells recovered from in vivo damage also show increased glycolysis and proliferation rate. - Abstract: Instead of relying on mitochondrial oxidative phosphorylation, most cancer cells rely heavily on aerobic glycolysis, a phenomenon termed as “the Warburg effect”. We considered that this effect is a direct consequence of damage which persists in cancer cells that recovermore » from damage. To this end, we studied glycolysis and rate of cell proliferation in cancer cells that recovered from severe damage. We show that in vitro Damage-Recovered (DR) cells exhibit mitochondrial structural remodeling, display Warburg effect, and show increased in vitro and in vivo proliferation and tolerance to damage. To test whether cancer cells derived from tumor microenvironment can show similar properties, we isolated Damage-Recovered (T{sup DR}) cells from tumors. We demonstrate that T{sup DR} cells also show increased aerobic glycolysis and a high proliferation rate. These findings show that Warburg effect and its consequences are induced in cancer cells that survive severe damage.« less

Triacylglycerols determine the unusual storage physiology of Cuphea seed.

PubMed

Crane, Jennifer; Miller, Annette L; van Roekel, J William; Walters, Christina

2003-09-01

Many species within the genus Cuphea (Lythraceae) produce seed with high levels of medium-chain fatty acids. Seeds of some Cuphea species lose viability when placed into storage at -18 degrees C. These species tolerate significant drying to 0.05 g/g and may, therefore, be intermediate in their storage characteristics. The thermal properties of seed lipids were observed using differential scanning calorimetry. Species with peak lipid melting temperatures >/=27 degrees C were found to be sensitive to -18 degrees C exposure while those with melting temperatures <27 degrees C were able to tolerate low-temperature exposure. This relationship was determined by the triacylglycerol composition of the individual species. Sensitive species have high concentrations of lauric acid (C(12)) and/or myristic acid (C(14)). Species with high concentrations of capric (C(8)) or caprylic acid (C(10)) or with high concentrations of unsaturated fatty acids tolerate low temperature exposure. Potential damage caused by low temperature exposure can be avoided by exposing seeds to a brief heat pulse of 45 degrees C to melt solidified lipids prior to imbibition. The relationship between the behavior of triacylglycerols in vivo, seed storage behavior and sensitivity to imbibitional damage is previously unreported and may apply to other species with physiologies that make them difficult to store.

Damage tolerance of candidate thermoset composites for use on single stage to orbit vehicles

NASA Technical Reports Server (NTRS)

Nettles, A. T.; Lance, D.; Hodge, A.

1994-01-01

Four fiber/resin systems were compared for resistance to damage and damage tolerance. One toughened epoxy and three toughened bismaleimide (BMI) resins were used, all with IM7 carbon fiber reinforcement. A statistical design of experiments technique was used to evaluate the effects of impact energy, specimen thickness, and impactor diameter on the damage area, as computed by C-scans, and residual compression-after-impact (CAI) strength. Results showed that two of the BMI systems sustained relatively large damage zones yet had an excellent retention of CAI strength.

Impact testing of textile composite materials

NASA Technical Reports Server (NTRS)

Portanova, Marc

1995-01-01

The objectives of this report were to evaluate the impact damage resistance and damage tolerance of a variety of textile composite materials. Static indentation and impact tests were performed on the stitched and unstitched uniweave composites constructed from AS4/3501-6 Carbon/Epoxy with a fiberglass yarn woven in to hold the fibers together while being stitched. Compression and tension were measured after the tests to determine the damage resistance, residual strength and the damage tolerance of the specimens.

Mechanical behavior, damage tolerance and durability of fiber metal laminates for aircraft structures

NASA Astrophysics Data System (ADS)

Wu, Guocai

This study systematically explores the mechanical behavior, damage tolerance and durability of fiber metal laminates, a promising candidate materials system for next generation aerospace structures. The experimental results indicated that GLARE laminates exhibited a bilinear deformation behavior under static in-plane loading. Both an analytical constitutive model based on a modified classical lamination theory which incorporates the elasto-plastic behavior of aluminum alloy and a numerical simulation based on finite element modeling are used to predict the nonlinear stress-strain response and deformation behavior of GLARE laminates. The blunt notched strength of GLARE laminates increased with decreasing specimen width and decreasing hole diameter. The notched strength of GLARE laminates was evaluated based on a modified point stress criterion. A computer simulation based on finite element method was performed to study stress concentration and distribution around the notch and verify the analytical and experimental results of notched strength. Good agreement is obtained between the model predictions and experimental results. Experimental results also indicate that GLARE laminates exhibited superior impact properties to those of monolithic 2024-T3 aluminum alloy at low velocity impact loading. The GLARE 5-2/1 laminate with 0°/90°/90°/0° fiber configuration exhibits a better impact resistance than the GLARE 4-3/2 laminate with 0°/90°/0° fiber orientation. The characteristic impact energies, the damage area, and the permanent deflection of laminates are used to evaluate the impact damage resistance. The post-impact residual tensile strength under various damage states ranging from the plastic dent, barely visible impact damage (BVID), clearly visible impact damage (CVID) up to the complete perforation was also measured and compared. The post-impact fatigue behavior under various stress levels and impact damage states was extensively explored. The damage initiation and progression, failure modes and crack propagation under different loading conditions were investigated and identified with microscopy, SEM, X-ray radiography, and by chemically removing outer aluminum layers.

Thermoplastic composite parts manufacture at Du Pont

NASA Astrophysics Data System (ADS)

Medwin, Steven J.; Coyle, Edward J.

1993-01-01

Low-cost routes to the manufacture of complex shaped composite parts have been defined using metal forming techniques and Du Pont's long discontinuous fiber (LDF) Technology. These manufacturing techniques include roll forming, stretch forming, and press forming. Near equivalence between the static, dynamic, and damage tolerance properties of LDF and continuous fiber composites have been demonstrated. Several examples are cited which demonstrate the potential for this technology to significantly reduce the cost of aerospace components.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lu, Chenyang; Niu, Liangliang; Chen, Nanjun

A grand challenge in material science is to understand the correlation between intrinsic properties and defect dynamics. Radiation tolerant materials are in great demand for safe operation and advancement of nuclear and aerospace systems. Unlike traditional approaches that rely on microstructural and nanoscale features to mitigate radiation damage, this study demonstrates enhancement of radiation tolerance with the suppression of void formation by two orders magnitude at elevated temperatures in equiatomic single-phase concentrated solid solution alloys, and more importantly, reveals its controlling mechanism through a detailed analysis of the depth distribution of defect clusters and an atomistic computer simulation. The enhancedmore » swelling resistance is attributed to the tailored interstitial defect cluster motion in the alloys from a long-range one-dimensional mode to a short-range three-dimensional mode, which leads to enhanced point defect recombination. Finally, the results suggest design criteria for next generation radiation tolerant structural alloys.« less

Defect evolution in ZnO and its effect on radiation tolerance.

PubMed

Lv, Jinpeng; Li, Xingji

2018-05-03

The origin of ZnO radiation resistance is fascinating but still unclear. Herein, we found that radiation tolerance of ZnO can be tuned by engineering intrinsic defects into the ZnO. The role played by native defects in the radiation tolerance of ZnO was systematically explored by carrying out N+ implantation on a set of home-grown ZnO nanocrystals with various lattice defect types and concentrations. Interestingly, decreasing the VO and Zni concentration significantly aggravated N+ radiation damage, indicating the presence of O-deficient defects to be the potential cause of the radiation hardness of ZnO. A similar phenomenon was also observed for H+-implanted ZnO. This work offers a new way to manipulate ZnO and endow it with desired physicochemical properties, and is expected to pave the way for its application in radiative environments.

Mechanistic Distinctions between CHK1 and WEE1 Inhibition Guide the Scheduling of Triple Therapy with Gemcitabine.

PubMed

Koh, Siang-Boon; Wallez, Yann; Dunlop, Charles R; Bernaldo de Quirós Fernández, Sandra; Bapiro, Tashinga E; Richards, Frances M; Jodrell, Duncan I

2018-06-01

Combination of cytotoxic therapy with emerging DNA damage response inhibitors (DDRi) has been limited by tolerability issues. However, the goal of most combination trials has been to administer DDRi with standard-of-care doses of chemotherapy. We hypothesized that mechanism-guided treatment scheduling could reduce the incidence of dose-limiting toxicities and enable tolerable multitherapeutic regimens. Integrative analyses of mathematical modeling and single-cell assays distinguished the synergy kinetics of WEE1 inhibitor (WEE1i) from CHEK1 inhibitor (CHK1i) by potency, spatiotemporal perturbation, and mitotic effects when combined with gemcitabine. These divergent properties collectively supported a triple-agent strategy, whereby a pulse of gemcitabine and CHK1i followed by WEE1i durably suppressed tumor cell growth. In xenografts, CHK1i exaggerated replication stress without mitotic CDK hyperactivation, enriching a geminin-positive subpopulation and intratumoral gemcitabine metabolite. Without overt toxicity, addition of WEE1i to low-dose gemcitabine and CHK1i was most effective in tumor control compared with single and double agents. Overall, our work provides quantitative insights into the mechanisms of DDRi chemosensitization, leading to the rational development of a tolerable multitherapeutic regimen. Significance: Multiple lines of mechanistic insight regarding DNA damage response inhibitors rationally guide the preclinical development of a tolerable multitherapeutic regimen. Graphical Abstract: http://cancerres.aacrjournals.org/content/canres/78/11/3054/F1.large.jpg Cancer Res; 78(11); 3054-66. ©2018 AACR . ©2018 American Association for Cancer Research.

Fuel containment, lightning protection and damage tolerance in large composite primary aircraft structures

NASA Technical Reports Server (NTRS)

Griffin, Charles F.; James, Arthur M.

1985-01-01

The damage-tolerance characteristics of high strain-to-failure graphite fibers and toughened resins were evaluated. Test results show that conventional fuel tank sealing techniques are applicable to composite structures. Techniques were developed to prevent fuel leaks due to low-energy impact damage. For wing panels subjected to swept stroke lightning strikes, a surface protection of graphite/aluminum wire fabric and a fastener treatment proved effective in eliminating internal sparking and reducing structural damage. The technology features developed were incorporated and demonstrated in a test panel designed to meet the strength, stiffness, and damage tolerance requirements of a large commercial transport aircraft. The panel test results exceeded design requirements for all test conditions. Wing surfaces constructed with composites offer large weight savings if design allowable strains for compression can be increased from current levels.

Self-repairing composites for airplane components

NASA Astrophysics Data System (ADS)

Dry, Carolyn

2008-03-01

Durability and damage tolerance criteria drives the design of most composite structures. Those criteria could be altered by developing structure that repairs itself from impact damage. This is a technology for increasing damage tolerance for impact damage. Repaired damage would enable continued function and prevent further degradation to catastrophic failure in the case of an aircraft application. Further, repaired damage would enable applications to be utilized without reduction in performance due to impacts. Self repairing structures are designed to incorporate hollow fibers, which will release a repairing agent when the structure is impacted, so that the repairing agent will fill delaminations, voids and cracks in les than one minute, thus healing matrix voids. The intent is to modify the durability and damage tolerance criteria by incorporation of self-healing technologies to reduce overall weight: The structure will actually remain lighter than current conventional design procedures allow. Research objective(s) were: Prove that damage can be repaired to within 80-90% of original flexural strength in less than one minute, in laminates that are processed at 300-350F typical for aircraft composites. These were successfully met. The main focus was on testing of elements in compression after impact and a larger component in shear at Natural Process Design, Inc. Based on these results the advantages purposes are assessed. The results show potential; with self repairing composites, compressive strength is maintained sufficiently so that less material can be used as per durability and damage tolerance, yielding a lighter structure.

Damage tolerance assessment handbook. Volume 1 : introduction, fracture mechanics, fatigue crack propagation

DOT National Transportation Integrated Search

1999-02-01

The handbook is presented in two volumes. This volume, Volume I, introduces the damage tolerance concept with an historical perspective followed by the fundamentals of fracture mechanics and fatigue crack propagation. Various fracture criteria and cr...

Continuum damage modeling and simulation of hierarchical dental enamel

NASA Astrophysics Data System (ADS)

Ma, Songyun; Scheider, Ingo; Bargmann, Swantje

2016-05-01

Dental enamel exhibits high fracture toughness and stiffness due to a complex hierarchical and graded microstructure, optimally organized from nano- to macro-scale. In this study, a 3D representative volume element (RVE) model is adopted to study the deformation and damage behavior of the fibrous microstructure. A continuum damage mechanics model coupled to hyperelasticity is developed for modeling the initiation and evolution of damage in the mineral fibers as well as protein matrix. Moreover, debonding of the interface between mineral fiber and protein is captured by employing a cohesive zone model. The dependence of the failure mechanism on the aspect ratio of the mineral fibers is investigated. In addition, the effect of the interface strength on the damage behavior is studied with respect to geometric features of enamel. Further, the effect of an initial flaw on the overall mechanical properties is analyzed to understand the superior damage tolerance of dental enamel. The simulation results are validated by comparison to experimental data from micro-cantilever beam testing at two hierarchical levels. The transition of the failure mechanism at different hierarchical levels is also well reproduced in the simulations.

Design of novel materials for additive manufacturing - Isotropic microstructure and high defect tolerance.

PubMed

Günther, J; Brenne, F; Droste, M; Wendler, M; Volkova, O; Biermann, H; Niendorf, T

2018-01-22

Electron Beam Melting (EBM) is a powder-bed additive manufacturing technology enabling the production of complex metallic parts with generally good mechanical properties. However, the performance of powder-bed based additively manufactured materials is governed by multiple factors that are difficult to control. Alloys that solidify in cubic crystal structures are usually affected by strong anisotropy due to the formation of columnar grains of preferred orientation. Moreover, processing induced defects and porosity detrimentally influence static and cyclic mechanical properties. The current study presents results on processing of a metastable austenitic CrMnNi steel by EBM. Due to multiple phase transformations induced by intrinsic heat-treatment in the layer-wise EBM process the material develops a fine-grained microstructure almost without a preferred crystallographic grain orientation. The deformation-induced phase transformation yields high damage tolerance and, thus, excellent mechanical properties less sensitive to process-induced inhomogeneities. Various scan strategies were applied to evaluate the width of an appropriate process window in terms of microstructure evolution, porosity and change of chemical composition.

Improved Accident Tolerance of Austenitic Stainless Steel Cladding through Colossal Supersaturation with Interstitial Solutes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ernst, Frank

We proposed a program-supporting research project in the area of fuel-cycle R&D, specifically on the topic of advanced fuels. Our goal was to investigate whether SECIS (surface engineering by concentrated interstitial solute – carbon, nitrogen) can improve the properties of austenitic stainless steels and related structural alloys such that they can be used for nuclear fuel cladding in LWRs (light-water reactors) and significantly excel currently used alloys with regard to performance, safety, service life, and accident tolerance. We intended to demonstrate that SECIS can be adapted for post-processing of clad tubing to significantly enhance mechanical properties (hardness, wear resistance, andmore » fatigue life), corrosion resistance, resistance to stress–corrosion cracking (hydrogen-induced embrittlement), and – potentially – radiation resistance (against electron-, neutron-, or ion-radiation damage). To test this hypothesis, we measured various relevant properties of the surface-engineered alloys and compared them with corresponding properties of the non–treated, as-received alloys. In particular, we studied the impact of heat exposure corresponding to BWR (boiling-water reactor) working and accident (loss-of-coolant) conditions and the effect of ion irradiation.« less

A robust salt-tolerant superoleophobic alginate/graphene oxide aerogel for efficient oil/water separation in marine environments

NASA Astrophysics Data System (ADS)

Li, Yuqi; Zhang, Hui; Fan, Mizi; Zheng, Peitao; Zhuang, Jiandong; Chen, Lihui

2017-04-01

Marine pollution caused by frequent oil spill accidents has brought about tremendous damages to marine ecological environment. Therefore, the facile large-scale preparation of three-dimensional (3D) porous functional materials with special wettability is in urgent demand. In this study, we report a low-cost and salt-tolerant superoleophobic aerogel for efficient oil/seawater separation. The aerogel is prepared through incorporating graphene oxide (GO) into alginate (ALG) matrix by using a facile combined freeze-drying and ionic cross-linking method. The 3D structure interconnected by ALG and GO ensures the high mechanical strength and good flexibility of the developed aerogel. The rough microstructure combined with the hydrophilicity of the aerogel ensures its excellent underwater superoleophobic and antifouling properties. High-content polysaccharides contained in the aerogel guarantees its excellent salt-tolerant property. More impressively, the developed aerogel can retain its underwater superoleophobicity even after 30 days of immersion in seawater, indicating its good stability in marine environments. Furthermore, the aerogel could separate various oil/water mixtures with high separation efficiency (>99%) and good reusability (at least 40 cycles). The facile fabrication process combined with the excellent separation performance makes it promising for practical applications in marine environments.

Delamination Assessment Tool for Spacecraft Composite Structures

NASA Astrophysics Data System (ADS)

Portela, Pedro; Preller, Fabian; Wittke, Henrik; Sinnema, Gerben; Camanho, Pedro; Turon, Albert

2012-07-01

Fortunately only few cases are known where failure of spacecraft structures due to undetected damage has resulted in a loss of spacecraft and launcher mission. However, several problems related to damage tolerance and in particular delamination of composite materials have been encountered during structure development of various ESA projects and qualification testing. To avoid such costly failures during development, launch or service of spacecraft, launcher and reusable launch vehicles structures a comprehensive damage tolerance verification approach is needed. In 2009, the European Space Agency (ESA) initiated an activity called “Delamination Assessment Tool” which is led by the Portuguese company HPS Lda and includes academic and industrial partners. The goal of this study is the development of a comprehensive damage tolerance verification approach for launcher and reusable launch vehicles (RLV) structures, addressing analytical and numerical methodologies, material-, subcomponent- and component testing, as well as non-destructive inspection. The study includes a comprehensive review of current industrial damage tolerance practice resulting from ECSS and NASA standards, the development of new Best Practice Guidelines for analysis, test and inspection methods and the validation of these with a real industrial case study. The paper describes the main findings of this activity so far and presents a first iteration of a Damage Tolerance Verification Approach, which includes the introduction of novel analytical and numerical tools at an industrial level. This new approach is being put to the test using real industrial case studies provided by the industrial partners, MT Aerospace, RUAG Space and INVENT GmbH

Probabilistic modeling of condition-based maintenance strategies and quantification of its benefits for airliners

NASA Astrophysics Data System (ADS)

Pattabhiraman, Sriram

Airplane fuselage structures are designed with the concept of damage tolerance, wherein small damage are allowed to remain on the airplane, and damage that otherwise affect the safety of the structure are repaired. The damage critical to the safety of the fuselage are repaired by scheduling maintenance at pre-determined intervals. Scheduling maintenance is an interesting trade-off between damage tolerance and cost. Tolerance of larger damage would require less frequent maintenance and hence, a lower cost, to maintain a certain level of reliability. Alternatively, condition-based maintenance techniques have been developed using on-board sensors, which track damage continuously and request maintenance only when the damage size crosses a particular threshold. This effects a tolerance of larger damage than scheduled maintenance, leading to savings in cost. This work quantifies the savings of condition-based maintenance over scheduled maintenance. The work also quantifies converting the cost savings into weight savings. Structural health monitoring will need time to be able to establish itself as a stand-alone system for maintenance, due to concerns on its diagnosis accuracy and reliability. This work also investigates the effect of synchronizing structural health monitoring system with scheduled maintenance. This work uses on-board SHM equipment skip structural airframe maintenance (a subsect of scheduled maintenance), whenever deemed unnecessary while maintain a desired level of safety of structure. The work will also predict the necessary maintenance for a fleet of airplanes, based on the current damage status of the airplanes. The work also analyses the possibility of false alarm, wherein maintenance is being requested with no critical damage on the airplane. The work use SHM as a tool to identify lemons in a fleet of airplanes. Lemons are those airplanes that would warrant more maintenance trips than the average behavior of the fleet.

Orchid flowers tolerance to gamma-radiation

NASA Astrophysics Data System (ADS)

Kikuchi, Olivia Kimiko

2000-03-01

Cut flowers are fresh goods that may be treated with fumigants such as methyl bromide to meet the needs of the quarantine requirements of importing countries. Irradiation is a non-chemical alternative to substitute the methyl bromide treatment of fresh products. In this research, different cut orchids were irradiated to examine their tolerance to gamma-rays. A 200 Gy dose did inhibit the Dendrobium palenopsis buds from opening, but did not cause visible damage to opened flowers. Doses of 800 and 1000 Gy were damaging because they provoked the flowers to drop from the stem. Cattleya irradiated with 750 Gy did not show any damage, and were therefore eligible for the radiation treatment. Cymbidium tolerated up to 300 Gy and above this dose dropped prematurely. On the other hand, Oncydium did not tolerate doses above 150 Gy.

Conducting field trials for frost tolerance breeding in cereals.

PubMed

Cattivelli, Luigi

2014-01-01

Cereal species can be damaged by frost either during winter or at flowering stage. Frost tolerance per se is only a part of the mechanisms that allow the plants to survive during winter; winterhardiness also considers other biotic or physical stresses that challenge the plants during the winter season limiting their survival rate. While frost tolerance can also be tested in controlled environments, winterhardiness can be determined only with field evaluations. Post-heading frost damage occurs from radiation frost events in spring during the reproductive stages. A reliable evaluation of winterhardiness or of post-heading frost damage should be carried out with field trials replicated across years and locations to overcome the irregular occurrence of natural conditions which satisfactorily differentiate genotypes. The evaluation of post-heading frost damage requires a specific attention to plant phenology. The extent of frost damage is usually determined with a visual score at the end of the winter.

Superalloy Lattice Block Structures

NASA Technical Reports Server (NTRS)

Nathal, M. V.; Whittenberger, J. D.; Hebsur, M. G.; Kantzos, P. T.; Krause, D. L.

2004-01-01

Initial investigations of investment cast superalloy lattice block suggest that this technology will yield a low cost approach to utilize the high temperature strength and environmental resistance of superalloys in lightweight, damage tolerant structural configurations. Work to date has demonstrated that relatively large superalloy lattice block panels can be successfully investment cast from both IN-718 and Mar-M247. These castings exhibited mechanical properties consistent with the strength of the same superalloys measured from more conventional castings. The lattice block structure also accommodates significant deformation without failure, and is defect tolerant in fatigue. The potential of lattice block structures opens new opportunities for the use of superalloys in future generations of aircraft applications that demand strength and environmental resistance at elevated temperatures along with low weight.

Damage Mechanisms and Mechanical Properties of High-Strength Multiphase Steels

PubMed Central

Heibel, Sebastian; Dettinger, Thomas; Nester, Winfried; Tekkaya, A. Erman

2018-01-01

The usage of high-strength steels for structural components and reinforcement parts is inevitable for modern car-body manufacture in reaching lightweight design as well as increasing passive safety. Depending on their microstructure these steels show differing damage mechanisms and various mechanical properties which cannot be classified comprehensively via classical uniaxial tensile testing. In this research, damage initiation, evolution and final material failure are characterized for commercially produced complex-phase (CP) and dual-phase (DP) steels in a strength range between 600 and 1000 MPa. Based on these investigations CP steels with their homogeneous microstructure are characterized as damage tolerant and hence less edge-crack sensitive than DP steels. As final fracture occurs after a combination of ductile damage evolution and local shear band localization in ferrite grains at a characteristic thickness strain, this strain measure is introduced as a new parameter for local formability. In terms of global formability DP steels display advantages because of their microstructural composition of soft ferrite matrix including hard martensite particles. Combining true uniform elongation as a measure for global formability with the true thickness strain at fracture for local formability the mechanical material response can be assessed on basis of uniaxial tensile testing incorporating all microstructural characteristics on a macroscopic scale. Based on these findings a new classification scheme for the recently developed high-strength multiphase steels with significantly better formability resulting of complex underlying microstructures is introduced. The scheme overcomes the steel designations using microstructural concepts, which provide no information about design and production properties. PMID:29747417

Tensile strength of composite sheets with unidirectional stringers and crack-like damage

NASA Technical Reports Server (NTRS)

Poe, C. C., Jr.

1984-01-01

The damage tolerance characteristics of metal tension panels with riveted and bonded stringers are well known. The stringers arrest unstable cracks and retard propagation of fatigue cracks. Residual strengths and fatigue lives are considerably greater than those of unstiffened or integrally stiffened sheets. The damage tolerance of composite sheets with bonded composite stringers loaded in tension was determined. Cracks in composites do not readily propagate in fatigue, at least not through fibers. Moreover, the residual strength of notched composites is sometimes even increased by fatigue loading. Therefore, the residual strength aspect of damage tolerance, and not fatigue crack propagation, was investigated. About 50 graphite/epoxy composite panels were made with two sheet layups and several stringer configurations. Crack-like slots were cut in the middle of the panels to simulate damage. The panels were instrumented and monotonically loaded in tension to failure. The tests indicate that the composite panels have considerable damage tolerance, much like metal panels. The stringers arrested cracks that ran from the crack-like slots, and the residual strengths were considerably greater than those of unstiffened composite sheets. A stress intensity factor analysis was developed to predict the failing strains of the stiffened panels. Using the analysis, a single design curve was produced for composite sheets with bonded stringers of any configuration.

Test and analysis results for composite transport fuselage and wing structures

NASA Technical Reports Server (NTRS)

Deaton, Jerry W.; Kullerd, Susan M.; Madan, Ram C.; Chen, Victor L.

1992-01-01

Automated tow placement (ATP) and stitching of dry textile composite preforms followed by resin transfer molding (RTM) are being studied as cost effective manufacturing processes for obtaining damage tolerant fuselage and wing structures for transport aircraft. Data are presented to assess the damage tolerance of ATP and RTM fuselage elements with stitched-on stiffeners from compression tests of impacted three J-stiffened panels and from stiffener pull-off tests. Data are also presented to assess the damage tolerance of RTM wing elements which had stitched skin and stiffeners from impacted single stiffener and three blade stiffened compression tests and stiffener pull-off tests.

Mitochondrial DNA repair and damage tolerance.

PubMed

Stein, Alexis; Sia, Elaine A

2017-01-01

The accurate maintenance of mitochondrial DNA (mtDNA) is required in order for eukaryotic cells to assemble a functional electron transport chain. This independently-maintained genome relies on nuclear-encoded proteins that are imported into the mitochondria to carry out replication and repair processes. Decades of research has made clear that mitochondria employ robust and varied mtDNA repair and damage tolerance mechanisms in order to ensure the proper maintenance of the mitochondrial genome. This review focuses on our current understanding of mtDNA repair and damage tolerance pathways including base excision repair, mismatch repair, homologous recombination, non-homologous end joining, translesion synthesis and mtDNA degradation in both yeast and mammalian systems.

77 FR 50576 - Damage Tolerance and Fatigue Evaluation of Composite Rotorcraft Structures; OMB Approval of...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-22

... Composite Rotorcraft Structures; OMB Approval of Information Collection AGENCY: Federal Aviation... requirement contained in the FAA's final rule, ``Damage Tolerance and Fatigue Evaluation of Composite... and Fatigue Evaluation of Composite Rotorcraft Structures,'' published in the Federal Register (76 FR...

Cumulative herbivory outpaces compensation for early floral damage on a monocarpic perennial thistle

USDA-ARS?s Scientific Manuscript database

Floral herbivory presents a threat to plant reproductive success. Monocarpic plants should tolerate early apical damage with compensatory reproductive effort by subsequent flower heads during their single flowering season. However, the actual contribution of this tolerance response to net fitness is...

Improving glucose tolerance by muscle-damaging exercise.

PubMed

Ho, Chien-Te; Otaka, Machiko; Kuo, Chia-Hua

2017-04-01

Tissue damage is regarded as an unwanted medical condition to be avoided. However, introducing tolerable tissue damages has been used as a therapeutic intervention in traditional and complementary medicine to cure discomfort and illness. Eccentric exercise is known to cause significant necrosis and insulin resistance of skeletal muscle. The purpose of this study was to determine the magnitude of muscle damage and blood glucose responses during an oral glucose tolerance test (OGTT) after eccentric training in 21 young participants. They were challenged by 5 times of 100-meter downhill sprinting and 20 times of squats training at 30 pounds weight load for 3 days, which resulted in a wide spectrum of muscle creatine kinase (CK) surges in plasma, 48 h after the last bout of exercise. Participants were then divided into two groups according the magnitude of CK increases (low CK: +48% ± 0.3; high CK: +137% ± 0.5, P < 0.05). Both groups show comparable decreases in blood glucose levels in OGTT, suggesting that this muscle-damaging exercise does not appear to decrease but rather improve glycemic control in men. The result of the study rejects the hypothesis that eccentric exercise decreases glucose tolerance. Improved glucose tolerance with CK increase implicates a beneficial effect of replacing metabolically weaker muscle fibers by eccentric exercise in Darwinian natural selection fashion.

Progressive failure methodologies for predicting residual strength and life of laminated composites

NASA Technical Reports Server (NTRS)

Harris, Charles E.; Allen, David H.; Obrien, T. Kevin

1991-01-01

Two progressive failure methodologies currently under development by the Mechanics of Materials Branch at NASA Langley Research Center are discussed. The damage tolerance/fail safety methodology developed by O'Brien is an engineering approach to ensuring adequate durability and damage tolerance by treating only delamination onset and the subsequent delamination accumulation through the laminate thickness. The continuum damage model developed by Allen and Harris employs continuum damage laws to predict laminate strength and life. The philosophy, mechanics framework, and current implementation status of each methodology are presented.

Increasing intracellular trehalose is sufficient to confer desiccation tolerance to Saccharomyces cerevisiae

PubMed Central

Tapia, Hugo; Young, Lindsey; Fox, Douglas; Bertozzi, Carolyn R.; Koshland, Douglas

2015-01-01

Diverse organisms capable of surviving desiccation, termed anhydrobiotes, include species from bacteria, yeast, plants, and invertebrates. However, most organisms are sensitive to desiccation, likely due to an assortment of different stresses such as protein misfolding and aggregation, hyperosmotic stress, membrane fracturing, and changes in cell volume and shape leading to an overcrowded cytoplasm and metabolic arrest. The exact stress(es) that cause lethality in desiccation-sensitive organisms and how the lethal stresses are mitigated in desiccation-tolerant organisms remain poorly understood. The presence of trehalose in anhydrobiotes has been strongly correlated with desiccation tolerance. In the yeast Saccharomyces cerevisiae, trehalose is essential for survival after long-term desiccation. Here, we establish that the elevation of intracellular trehalose in dividing yeast by its import from the media converts yeast from extreme desiccation sensitivity to a high level of desiccation tolerance. This trehalose-induced tolerance is independent of utilization of trehalose as an energy source, de novo synthesis of other stress effectors, or the metabolic effects of trehalose biosynthetic intermediates, indicating that a chemical property of trehalose is directly responsible for desiccation tolerance. Finally, we demonstrate that elevated intracellular maltose can also make dividing yeast tolerant to short-term desiccation, indicating that other disaccharides have stress effector activity. However, trehalose is much more effective than maltose at conferring tolerance to long-term desiccation. The effectiveness and sufficiency of trehalose as an antagonizer of desiccation-induced damage in yeast emphasizes its potential to confer desiccation tolerance to otherwise sensitive organisms. PMID:25918381

Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein

PubMed Central

Hashimoto, Takuma; Horikawa, Daiki D.; Saito, Yuki; Kuwahara, Hirokazu; Kozuka-Hata, Hiroko; Shin-I, Tadasu; Minakuchi, Yohei; Ohishi, Kazuko; Motoyama, Ayuko; Aizu, Tomoyuki; Enomoto, Atsushi; Kondo, Koyuki; Tanaka, Sae; Hara, Yuichiro; Koshikawa, Shigeyuki; Sagara, Hiroshi; Miura, Toru; Yokobori, Shin-ichi; Miyagawa, Kiyoshi; Suzuki, Yutaka; Kubo, Takeo; Oyama, Masaaki; Kohara, Yuji; Fujiyama, Asao; Arakawa, Kazuharu; Katayama, Toshiaki; Toyoda, Atsushi; Kunieda, Takekazu

2016-01-01

Tardigrades, also known as water bears, are small aquatic animals. Some tardigrade species tolerate almost complete dehydration and exhibit extraordinary tolerance to various physical extremes in the dehydrated state. Here we determine a high-quality genome sequence of Ramazzottius varieornatus, one of the most stress-tolerant tardigrade species. Precise gene repertoire analyses reveal the presence of a small proportion (1.2% or less) of putative foreign genes, loss of gene pathways that promote stress damage, expansion of gene families related to ameliorating damage, and evolution and high expression of novel tardigrade-unique proteins. Minor changes in the gene expression profiles during dehydration and rehydration suggest constitutive expression of tolerance-related genes. Using human cultured cells, we demonstrate that a tardigrade-unique DNA-associating protein suppresses X-ray-induced DNA damage by ∼40% and improves radiotolerance. These findings indicate the relevance of tardigrade-unique proteins to tolerability and tardigrades could be a bountiful source of new protection genes and mechanisms. PMID:27649274

Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein.

PubMed

Hashimoto, Takuma; Horikawa, Daiki D; Saito, Yuki; Kuwahara, Hirokazu; Kozuka-Hata, Hiroko; Shin-I, Tadasu; Minakuchi, Yohei; Ohishi, Kazuko; Motoyama, Ayuko; Aizu, Tomoyuki; Enomoto, Atsushi; Kondo, Koyuki; Tanaka, Sae; Hara, Yuichiro; Koshikawa, Shigeyuki; Sagara, Hiroshi; Miura, Toru; Yokobori, Shin-Ichi; Miyagawa, Kiyoshi; Suzuki, Yutaka; Kubo, Takeo; Oyama, Masaaki; Kohara, Yuji; Fujiyama, Asao; Arakawa, Kazuharu; Katayama, Toshiaki; Toyoda, Atsushi; Kunieda, Takekazu

2016-09-20

Tardigrades, also known as water bears, are small aquatic animals. Some tardigrade species tolerate almost complete dehydration and exhibit extraordinary tolerance to various physical extremes in the dehydrated state. Here we determine a high-quality genome sequence of Ramazzottius varieornatus, one of the most stress-tolerant tardigrade species. Precise gene repertoire analyses reveal the presence of a small proportion (1.2% or less) of putative foreign genes, loss of gene pathways that promote stress damage, expansion of gene families related to ameliorating damage, and evolution and high expression of novel tardigrade-unique proteins. Minor changes in the gene expression profiles during dehydration and rehydration suggest constitutive expression of tolerance-related genes. Using human cultured cells, we demonstrate that a tardigrade-unique DNA-associating protein suppresses X-ray-induced DNA damage by ∼40% and improves radiotolerance. These findings indicate the relevance of tardigrade-unique proteins to tolerability and tardigrades could be a bountiful source of new protection genes and mechanisms.

Hydroxyurea therapy requires HbF induction for clinical benefit in a sickle cell mouse model

PubMed Central

Lebensburger, Jeffrey D.; Pestina, Tamara I.; Ware, Russell E.; Boyd, Kelli L.; Persons, Derek A.

2010-01-01

Hydroxyurea has proven clinical efficacy in patients with sickle cell disease. Potential mechanisms for the beneficial effects include fetal hemoglobin induction and the reduction of cell adhesive properties, inflammation and hypercoagulability. Using a murine model of sickle cell disease in which fetal hemoglobin induction does not occur, we evaluated whether hydroxyurea administration would still yield improvements in hematologic parameters and reduce end-organ damage. Animals given a maximally tolerated dose of hydroxyurea that resulted in significant reductions in the neutrophil and platelet counts showed no improvement in hemolytic anemia and end-organ damage compared to control mice. In contrast, animals having high levels of fetal hemoglobin due to gene transfer with a γ-globin lentiviral vector showed correction of anemia and organ damage. These data suggest that induction of fetal hemoglobin by hydroxyurea is an essential mechanism for its clinical benefits. PMID:20378564

Effects of chemical alternation on damage accumulation in concentrated solid-solution alloys

DOE PAGES

Ullah, Mohammad W.; Xue, Haizhou; Velisa, Gihan; ...

2017-06-23

Single-phase concentrated solid-solution alloys (SP-CSAs) have recently gained unprecedented attention due to their promising properties. To understand effects of alloying elements on irradiation-induced defect production, recombination and evolution, an integrated study of ion irradiation, ion beam analysis and atomistic simulations are carried out on a unique set of model crystals with increasing chemical complexity, from pure Ni to Ni 80Fe 20, Ni 50Fe 50, and Ni 80Cr 20 binaries, and to a more complex Ni 40Fe 40Cr 20 alloy. Both experimental and simulation results suggest that the binary and ternary alloys exhibit higher radiation resistance than elemental Ni. The modelingmore » work predicts that Ni 40Fe 40Cr 20 has the best radiation tolerance, with the number of surviving Frenkel pairs being factors of 2.0 and 1.4 lower than pure Ni and the 80:20 binary alloys, respectively. While the reduced defect mobility in SP-CSAs is identified as a general mechanism leading to slower growth of large defect clusters, the effect of specific alloying elements on suppression of damage accumulation is clearly demonstrated. This work suggests that concentrated solid-solution provides an effective way to enhance radiation tolerance by creating elemental alternation at the atomic level. The demonstrated chemical effects on defect dynamics may inspire new design principles of radiation-tolerant structural alloys for advanced energy systems.« less

Advanced information processing system

NASA Technical Reports Server (NTRS)

Lala, J. H.

1984-01-01

Design and performance details of the advanced information processing system (AIPS) for fault and damage tolerant data processing on aircraft and spacecraft are presented. AIPS comprises several computers distributed throughout the vehicle and linked by a damage tolerant data bus. Most I/O functions are available to all the computers, which run in a TDMA mode. Each computer performs separate specific tasks in normal operation and assumes other tasks in degraded modes. Redundant software assures that all fault monitoring, logging and reporting are automated, together with control functions. Redundant duplex links and damage-spread limitation provide the fault tolerance. Details of an advanced design of a laboratory-scale proof-of-concept system are described, including functional operations.

Plane shock loading on mono- and nano-crystalline silicon carbide

NASA Astrophysics Data System (ADS)

Branicio, Paulo S.; Zhang, Jingyun; Rino, José P.; Nakano, Aiichiro; Kalia, Rajiv K.; Vashishta, Priya

2018-03-01

The understanding of the nanoscale mechanisms of shock damage and failure in SiC is essential for its application in effective and damage tolerant coatings. We use molecular-dynamics simulations to investigate the shock properties of 3C-SiC along low-index crystallographic directions and in nanocrystalline samples with 5 nm and 10 nm grain sizes. The predicted Hugoniot in the particle velocity range of 0.1 km/s-6.0 km/s agrees well with experimental data. The shock response transitions from elastic to plastic, predominantly deformation twinning, to structural transformation to the rock-salt phase. The predicted strengths from 12.3 to 30.9 GPa, at the Hugoniot elastic limit, are in excellent agreement with experimental data.

A Comprehensive Study on Damage Tolerance Properties of Notched Composite Laminates.

DTIC Science & Technology

1988-02-01

of Applied Mechanics, Vol. 46, pp. 90-96, the strain energy release rate. 1979. [14] F. Delae and F. Erdogan , "Bosid,;d Orlhotropic REFERENCES Strips...with Cracks", Int. J. of Fracture, Vol. 15, [1] F. Erdogan and G.C. Sih, "On the Crack Extension in pp.33-364,1979. Plates Under Plane Loading and...1987. Vol. 34, pp. 967-974, 1967. (17] F. Erdogan , "Mixed Boundary-VL..t; Problems in [4] B. Cotterell, "The Influence of the Stress Distribution

Assessment of Damage Tolerance Requirements and Analysis. Volume 4. Raw Test Data

DTIC Science & Technology

1986-03-31

T3XX aluminum alloy . Four (4) product forms were selected: MATERIAL PRODUCT FORM THICKNESS (IN) 2024-T3 Sheet 0.090, 0.190 2024-T351 Plate 0.250 2024...T3511 Extruded Tee 0.190 2024-T3511 Extruded Angle 0.250 The material selected to represent bomber/transport/cargo aircraft is 7075- T6XX aluminum alloy ...aluminum alloys , respectively. The raw test data were processed in accordance with ASTM Standard E-8. All the tensile properties, except those marked

Is sexual reproduction of high-mountain plants endangered by heat?

PubMed

Ladinig, Ursula; Pramsohler, Manuel; Bauer, Ines; Zimmermann, Sonja; Neuner, Gilbert; Wagner, Johanna

2015-04-01

Strong solar irradiation in combination with still air and dry soil can cause prostrate high-mountain plants to heat up considerably and ultimately suffer heat damage. Such heat damage has been repeatedly shown for vegetative structures, but not for reproductive structures, which we expected to be particularly vulnerable to heat. Heat effects on cold-adapted plants may increase with rising global temperatures and the predicted increase in heat waves. We have tested the heat tolerance of reproductive versus vegetative shoots at different reproductive stages, comparing ten common plant species from different elevation belts in the European Alps. Plant samples were exposed to temperatures in 2-K steps of 30 min each between 42 and 56 °C. Heat damage was assessed by visual rating and vital staining. Reproductive shoots were on average 2.5 K less heat tolerant (LT50, i.e. the mean temperature causing 50 % heat damage, 47.2 °C) than vegetative shoots (mean LT50 49.7 °C). Initial heat injuries (mean LT10) were observed at 43-45 °C in heat-susceptible species and at 45-47 °C in more heat-tolerant species, in at least one reproductive stage. Generally, heat tolerance was significantly higher during fruiting than during the bud stages and anthesis. Prostrate species with acaulescent buds and flowers tolerated heat better than those with caulescent buds and flowers. Petals were the most heat-susceptible plant structure and mature pollen the most heat tolerant. Based on these data, heat tolerance of reproductive structures appears to be adapted to the prevailing maximum temperatures which the plants experience during different reproductive stages in their environment. During hot spells, however, heat tolerance thresholds may be exceeded. More frequent heat waves would decrease the reproductive output and, consequently, the competitiveness of heat-susceptible species.

Demonstrating damage tolerance of composite airframes

NASA Technical Reports Server (NTRS)

Poe, Clarence C., Jr.

1993-01-01

Commercial transport aircraft operating in the United States are certified by the Federal Aviation Authority to be damage tolerant. On 28 April 1988, Aloha Airlines Flight 243, a Boeing 727-200 airplane, suffered an explosive decompression of the fuselage but landed safely. This event provides very strong justification for the damage tolerant design criteria. The likely cause of the explosive decompression was the linkup of numerous small fatigue cracks that initiated at adjacent fastener holes in the lap splice joint at the side of the body. Actually, the design should have limited the damage size to less than two frame spacings (about 40 inches), but this type of 'multi-site damage' was not originally taken into account. This cracking pattern developed only in the high-time airplanes (many flights). After discovery in the fleet, a stringent inspection program using eddy current techniques was inaugurated to discover these cracks before they linked up. Because of concerns about safety and the maintenance burden, the lap-splice joints of these high-time airplanes are being modified to remove cracks and prevent new cracking; newer designs account for 'multi-site damage'.

Damage Tolerant Design for Cold-Section Turbine Engine Disks

DTIC Science & Technology

1981-06-01

Ti-6Al-4V Disks ......... .. 59 28. FIOO 2nd-Stage Fan Disk Designs ........ ................ .. 61 29. Fan Disk Tangential Stress Profile... 61 30. Life-Limiting Features of Damage-Tolerant Disk .......... ... 62 31. Disk Life Limits .... ...................... 62 32. Life Test...Stress Rati• Model ..... .......... .. 113 61 . Thick-Section Center-Notched Specimen ....... ............. .. 116 62. Bolthole Specimen

New discoveries linking transcription to DNA repair and damage tolerance pathways.

PubMed

Cohen, Susan E; Walker, Graham C

2011-01-01

In Escherichia coli, the transcription elongation factor NusA is associated with all elongating RNA polymerases where it functions in transcription termination and antitermination. Here, we review our recent results implicating NusA in the recruitment of DNA repair and damage tolerance mechanisms to sites of stalled transcription complexes.

Aerothermal performance and damage tolerance of a Rene 41 metallic standoff thermal protection system at Mach 6.7

NASA Technical Reports Server (NTRS)

Avery, D. E.

1984-01-01

A flight-weight, metallic thermal protection system (TPS) model applicable to Earth-entry and hypersonic-cruise vehicles was subjected to multiple cycles of both radiant and aerothermal heating in order to evaluate its aerothermal performance, structural integrity, and damage tolerance. The TPS was designed for a maximum operating temperature of 2060 R and featured a shingled, corrugation-stiffened corrugated-skin heat shield of Rene 41, a nickel-base alloy. The model was subjected to 10 radiant heating tests and to 3 radiant preheat/aerothermal tests. Under radiant-heating conditions with a maximum surface temperature of 2050 R, the TPS performed as designed and limited the primary structure away from the support ribs to temperatures below 780 R. During the first attempt at aerothermal exposure, a failure in the panel-holder test fixture severely damaged the model. However, two radiant preheat/aerothermal tests were made with the damaged model to test its damage tolerance. During these tests, the damaged area did not enlarge; however, the rapidly increasing structural temperature measuring during these tests indicates that had the damaged area been exposed to aerodynamic heating for the entire trajectory, an aluminum burn-through would have occurred.

Thermomechanical Fatigue Durability of T650-35/PMR-15 Sheet Molding Compound

NASA Technical Reports Server (NTRS)

Castelli, Michael G.; Sutter, James K.; Benson, Dianne

1998-01-01

Although polyimide based composites have been used for many years in a wide variety of elevated temperature applications, very little work has been done to examine the durability and damage behavior under more prototypical thermomechanical fatigue (TMF) loadings. Synergistic effects resulting from simultaneous temperature and load cycling can potentially lead to enhanced, if not unique, damage modes and contribute to a number of nonlinear deformation responses. The goal of this research was to examine the effects of a TMF loading spectrum, representative of a gas turbine engine compressor application, on a polyimide sheet molding compound (SMC). High performance SMCs present alternatives to prepreg forms with great potential for low cost component production through less labor intensive, more easily automated manufacturing. To examine the issues involved with TMF, a detailed experimental investigation was conducted to characterize the durability of a T650-35/PMR-15 SMC subjected to TMF mission cycle loadings. Fatigue damage progression was tracked through macroscopic deformation and elastic stiffness. Additional properties, such as the glass transition temperature (T(sub g) and dynamic mechanical properties were examined. The fiber distribution orientation was also characterized through a detailed quantitative image analysis. Damage tolerance was quantified on the basis of residual static tensile properties after a prescribed number of TMF missions. Detailed microstructural examinations were conducted using optical and scanning electron microscopy to characterize the local damage. The imposed baseline TMF missions had only a modest impact on inducing fatigue damage with no statistically significant degradation occurring in the measured macroscopic properties. Microstructural damage was, however, observed subsequent to 100 h of TMF cycling which consisted primarily of fiber debonding and transverse cracking local to predominantly transverse fiber bundles. The TMF loadings did introduce creep related effects (strain accumulation) which led to rupture in some of the more aggressive stress scenarios examined. In some cases this creep behavior occurred at temperatures in excess of 150 C below commonly cited values for T(sub g). Thermomechanical exploratory creep tests revealed that the SMC was subject to time dependent deformation at stress/temperature thresholds of 150 MPa/230 C and 170 MPa/180 C.

Protective effect of gangliosides on DNA in human spermatozoa exposed to cryopreservation.

PubMed

Gavella, Mirjana; Lipovac, Vaskresenija; Garaj-Vrhovac, Verica; Gajski, Goran

2012-01-01

Gangliosides, the sialic acid-containing glycosphyngolipids, are amphiphilic compounds which in micellar form affect the properties and functions of a cellular membrane. The aim of this study was to test whether exogenous gangliosides supplied to cryopreservation media before freezing could protect sperm cells from cryopreservation-induced DNA damage assessed by Comet assay. Additionally, to investigate whether gangliosides were also able to reduce membrane integrity damage, malonaldialdehyde as a measure of lipid peroxidation and sperm-specific lactate dehydrogenase-C4 activity as an enzyme marker of sperm membrane leakage were determined. The monosialogangliosides (GM1) and trisialogangliosides (GT1b) were examined at a concentration of 100 μM, which was above their respective critical micellar concentrations. Exogenous gangliosides were not found to protect sperm membrane from lipid peroxidation. However, a freezing-/thawing-induced increase in Comet parameters was equally significantly prevented by the presence of both GM1 and GT1b (P < .05), indicating that the ceramide moiety, rather than the polar groups, is involved in the protective ability of gangliosides. The observed phenomena suggest that ganglioside micelles could modulate hydrophobic properties of the sperm membrane responsible for better tolerance to DNA fragmentation, thus protecting DNA integrity from cryopreservation-induced damage.

Cell cycle stage-specific roles of Rad18 in tolerance and repair of oxidative DNA damage

PubMed Central

Yang, Yang; Durando, Michael; Smith-Roe, Stephanie L.; Sproul, Chris; Greenwalt, Alicia M.; Kaufmann, William; Oh, Sehyun; Hendrickson, Eric A.; Vaziri, Cyrus

2013-01-01

The E3 ubiquitin ligase Rad18 mediates tolerance of replication fork-stalling bulky DNA lesions, but whether Rad18 mediates tolerance of bulky DNA lesions acquired outside S-phase is unclear. Using synchronized cultures of primary human cells, we defined cell cycle stage-specific contributions of Rad18 to genome maintenance in response to ultraviolet C (UVC) and H2O2-induced DNA damage. UVC and H2O2 treatments both induced Rad18-mediated proliferating cell nuclear antigen mono-ubiquitination during G0, G1 and S-phase. Rad18 was important for repressing H2O2-induced (but not ultraviolet-induced) double strand break (DSB) accumulation and ATM S1981 phosphorylation only during G1, indicating a specific role for Rad18 in processing of oxidative DNA lesions outside S-phase. However, H2O2-induced DSB formation in Rad18-depleted G1 cells was not associated with increased genotoxin sensitivity, indicating that back-up DSB repair mechanisms compensate for Rad18 deficiency. Indeed, in DNA LigIV-deficient cells Rad18-depletion conferred H2O2-sensitivity, demonstrating functional redundancy between Rad18 and non-homologous end joining for tolerance of oxidative DNA damage acquired during G1. In contrast with G1-synchronized cultures, S-phase cells were H2O2-sensitive following Rad18-depletion. We conclude that although Rad18 pathway activation by oxidative lesions is not restricted to S-phase, Rad18-mediated trans-lesion synthesis by Polη is dispensable for damage-tolerance in G1 (because of back-up non-homologous end joining-mediated DSB repair), yet Rad18 is necessary for damage tolerance during S-phase. PMID:23295675

Assessment of the Damage Tolerance of Postbuckled Hat-Stiffened Panels Using Single-Stringer Specimens

NASA Technical Reports Server (NTRS)

Bisagni, Chiara; Vescovini, Riccardo; Davila, Carlos G.

2010-01-01

A procedure is proposed for the assessment of the damage tolerance and collapse of stiffened composite panels using a single-stringer compression specimen. The dimensions of the specimen are determined such that the specimen s nonlinear response and collapse are representative of an equivalent multi-stringer panel in compression. Experimental tests are conducted on specimens with and without an embedded delamination. A shell-based finite element model with intralaminar and interlaminar damage capabilities is developed to predict the postbuckling response as well as the damage evolution from initiation to collapse.

Mechanism of Radiation Damage Reduction in Equiatomic Multicomponent Single Phase Alloys.

PubMed

Granberg, F; Nordlund, K; Ullah, Mohammad W; Jin, K; Lu, C; Bei, H; Wang, L M; Djurabekova, F; Weber, W J; Zhang, Y

2016-04-01

Recently a new class of metal alloys, of single-phase multicomponent composition at roughly equal atomic concentrations ("equiatomic"), have been shown to exhibit promising mechanical, magnetic, and corrosion resistance properties, in particular, at high temperatures. These features make them potential candidates for components of next-generation nuclear reactors and other high-radiation environments that will involve high temperatures combined with corrosive environments and extreme radiation exposure. In spite of a wide range of recent studies of many important properties of these alloys, their radiation tolerance at high doses remains unexplored. In this work, a combination of experimental and modeling efforts reveals a substantial reduction of damage accumulation under prolonged irradiation in single-phase NiFe and NiCoCr alloys compared to elemental Ni. This effect is explained by reduced dislocation mobility, which leads to slower growth of large dislocation structures. Moreover, there is no observable phase separation, ordering, or amorphization, pointing to a high phase stability of this class of alloys.

Mechanism of Radiation Damage Reduction in Equiatomic Multicomponent Single Phase Alloys

NASA Astrophysics Data System (ADS)

Granberg, F.; Nordlund, K.; Ullah, Mohammad W.; Jin, K.; Lu, C.; Bei, H.; Wang, L. M.; Djurabekova, F.; Weber, W. J.; Zhang, Y.

2016-04-01

Recently a new class of metal alloys, of single-phase multicomponent composition at roughly equal atomic concentrations ("equiatomic"), have been shown to exhibit promising mechanical, magnetic, and corrosion resistance properties, in particular, at high temperatures. These features make them potential candidates for components of next-generation nuclear reactors and other high-radiation environments that will involve high temperatures combined with corrosive environments and extreme radiation exposure. In spite of a wide range of recent studies of many important properties of these alloys, their radiation tolerance at high doses remains unexplored. In this work, a combination of experimental and modeling efforts reveals a substantial reduction of damage accumulation under prolonged irradiation in single-phase NiFe and NiCoCr alloys compared to elemental Ni. This effect is explained by reduced dislocation mobility, which leads to slower growth of large dislocation structures. Moreover, there is no observable phase separation, ordering, or amorphization, pointing to a high phase stability of this class of alloys.

Mechanism of Radiation Damage Reduction in Equiatomic Multicomponent Single Phase Alloys

DOE PAGES

Granberg, F.; Nordlund, K.; Ullah, Mohammad W.; ...

2016-04-01

Recently a new class of metal alloys, of single-phase multicomponent composition at roughly equal atomic concentrations (“equiatomic”), have been shown to exhibit promising mechanical, magnetic, and corrosion resistance properties, in particular, at high temperatures. These features make them potential candidates for components of next-generation nuclear reactors and other high-radiation environments that will involve high temperatures combined with corrosive environments and extreme radiation exposure. In spite of a wide range of recent studies of many important properties of these alloys, their radiation tolerance at high doses remains unexplored. In this work, a combination of experimental and modeling efforts reveals a substantialmore » reduction of damage accumulation under prolonged irradiation in single-phase NiFe and NiCoCr alloys compared to elemental Ni. This effect is explained by reduced dislocation mobility, which leads to slower growth of large dislocation structures. Finally and moreover, there is no observable phase separation, ordering, or amorphization, pointing to a high phase stability of this class of alloys.« less

Development of COPVS for High pressure, In-Space, Cryogenic Fuel Storage

NASA Technical Reports Server (NTRS)

DeLay, Tom; Schneider, Judy; Dyess, Mark; Hastings, Chad; Noorda, Ryan; Noorda, Jared; Patterson, James

2008-01-01

Polymeric composite overwrapped pressure vessels (COPVs) provide an attractive material system to support developing commercial launch business and alternate fuel ventures. However to be able to design with these materials, the mechanical behavior of the materials must be understood with regards to processing, performance, damage tolerance, and environment. For the storage of cryogenic propellants, it is important to evaluate the materials performance and impact damage resistance at cryogenic temperatures in order to minimize weight and to ensure safety and reliability. To evaluate the ultimate performance, various polymeric COPV's have been statically burst tested at cryogenic conditions before and after exposure to irradiation. Materials selected for these COPVs were based on the measured mechanical properties of candidate resin systems and fibers that were also tested at cryogenic conditions before and after exposure to irradiation. The correlation of COPV burst pressures with the constituent material properties has proven to be a valuable screening method for selection of suitable candidate materials with resistance to material degradation due to exposure to temperature and radiation.

Materials and processes laboratory composite materials characterization task, part 1. Damage tolerance

NASA Technical Reports Server (NTRS)

Nettles, A. T.; Tucker, D. S.; Patterson, W. J.; Franklin, S. W.; Gordon, G. H.; Hart, L.; Hodge, A. J.; Lance, D. G.; Russel, S. S.

1991-01-01

A test run was performed on IM6/3501-6 carbon-epoxy in which the material was processed, machined into specimens, and tested for damage tolerance capabilities. Nondestructive test data played a major role in this element of composite characterization. A time chart was produced showing the time the composite material spent within each Branch or Division in order to identify those areas which produce a long turnaround time. Instrumented drop weight testing was performed on the specimens with nondestructive evaluation being performed before and after the impacts. Destructive testing in the form of cross-sectional photomicrography and compression-after-impact testing were used. Results show that the processing and machining steps needed to be performed more rapidly if data on composite material is to be collected within a reasonable timeframe. The results of the damage tolerance testing showed that IM6/3501-6 is a brittle material that is very susceptible to impact damage.

Optimization of Aerospace Structure Subject to Damage Tolerance Criteria

NASA Technical Reports Server (NTRS)

Akgun, Mehmet A.

1999-01-01

The objective of this cooperative agreement was to seek computationally efficient ways to optimize aerospace structures subject to damage tolerance criteria. Optimization was to involve sizing as well as topology optimization. The work was done in collaboration with Steve Scotti, Chauncey Wu and Joanne Walsh at the NASA Langley Research Center. Computation of constraint sensitivity is normally the most time-consuming step of an optimization procedure. The cooperative work first focused on this issue and implemented the adjoint method of sensitivity computation in an optimization code (runstream) written in Engineering Analysis Language (EAL). The method was implemented both for bar and plate elements including buckling sensitivity for the latter. Lumping of constraints was investigated as a means to reduce the computational cost. Adjoint sensitivity computation was developed and implemented for lumped stress and buckling constraints. Cost of the direct method and the adjoint method was compared for various structures with and without lumping. The results were reported in two papers. It is desirable to optimize topology of an aerospace structure subject to a large number of damage scenarios so that a damage tolerant structure is obtained. Including damage scenarios in the design procedure is critical in order to avoid large mass penalties at later stages. A common method for topology optimization is that of compliance minimization which has not been used for damage tolerant design. In the present work, topology optimization is treated as a conventional problem aiming to minimize the weight subject to stress constraints. Multiple damage configurations (scenarios) are considered. Each configuration has its own structural stiffness matrix and, normally, requires factoring of the matrix and solution of the system of equations. Damage that is expected to be tolerated is local and represents a small change in the stiffness matrix compared to the baseline (undamaged) structure. The exact solution to a slightly modified set of equations can be obtained from the baseline solution economically without actually solving the modified system. Sherrnan-Morrison-Woodbury (SMW) formulas are matrix update formulas that allow this. SMW formulas were therefore used here to compute adjoint displacements for sensitivity computation and structural displacements in damaged configurations.

A robust salt-tolerant superoleophobic alginate/graphene oxide aerogel for efficient oil/water separation in marine environments

PubMed Central

Li, Yuqi; Zhang, Hui; Fan, Mizi; Zheng, Peitao; Zhuang, Jiandong; Chen, Lihui

2017-01-01

Marine pollution caused by frequent oil spill accidents has brought about tremendous damages to marine ecological environment. Therefore, the facile large-scale preparation of three-dimensional (3D) porous functional materials with special wettability is in urgent demand. In this study, we report a low-cost and salt-tolerant superoleophobic aerogel for efficient oil/seawater separation. The aerogel is prepared through incorporating graphene oxide (GO) into alginate (ALG) matrix by using a facile combined freeze-drying and ionic cross-linking method. The 3D structure interconnected by ALG and GO ensures the high mechanical strength and good flexibility of the developed aerogel. The rough microstructure combined with the hydrophilicity of the aerogel ensures its excellent underwater superoleophobic and antifouling properties. High-content polysaccharides contained in the aerogel guarantees its excellent salt-tolerant property. More impressively, the developed aerogel can retain its underwater superoleophobicity even after 30 days of immersion in seawater, indicating its good stability in marine environments. Furthermore, the aerogel could separate various oil/water mixtures with high separation efficiency (>99%) and good reusability (at least 40 cycles). The facile fabrication process combined with the excellent separation performance makes it promising for practical applications in marine environments. PMID:28397862

Synthesis, Characterization And Modeling Of Functionally Graded Multifunctional Hybrid Composites For Extreme Environments

DTIC Science & Technology

2017-04-04

research thrust areas are designed to enable the development of reliable, damage tolerant, lightweight structures with excellent thermal management...46 2. RESEARCH THRUST AREA: MULTISCALE CHARACTERIZATION AND MODELING .................................... 56 2.1 DESIGN OF MATERIALS...The research thrust areas are designed to enable the development of reliable, damage tolerant, lightweight structures with excellent thermal

Modulation of inflammation and disease tolerance by DNA damage response pathways.

PubMed

Neves-Costa, Ana; Moita, Luis F

2017-03-01

The accurate replication and repair of DNA is central to organismal survival. This process is challenged by the many factors that can change genetic information such as replication errors and direct damage to the DNA molecule by chemical and physical agents. DNA damage can also result from microorganism invasion as an integral step of their life cycle or as collateral damage from host defense mechanisms against pathogens. Here we review the complex crosstalk of DNA damage response and immune response pathways that might be evolutionarily connected and argue that DNA damage response pathways can be explored therapeutically to induce disease tolerance through the activation of tissue damage control processes. Such approach may constitute the missing pillar in the treatment of critical illnesses caused by multiple organ failure, such as sepsis and septic shock. © 2016 Federation of European Biochemical Societies.

Verification of recursive probabilistic integration (RPI) method for fatigue life management using non-destructive inspections

NASA Astrophysics Data System (ADS)

Chen, Tzikang J.; Shiao, Michael

2016-04-01

This paper verified a generic and efficient assessment concept for probabilistic fatigue life management. The concept is developed based on an integration of damage tolerance methodology, simulations methods1, 2, and a probabilistic algorithm RPI (recursive probability integration)3-9 considering maintenance for damage tolerance and risk-based fatigue life management. RPI is an efficient semi-analytical probabilistic method for risk assessment subjected to various uncertainties such as the variability in material properties including crack growth rate, initial flaw size, repair quality, random process modeling of flight loads for failure analysis, and inspection reliability represented by probability of detection (POD). In addition, unlike traditional Monte Carlo simulations (MCS) which requires a rerun of MCS when maintenance plan is changed, RPI can repeatedly use a small set of baseline random crack growth histories excluding maintenance related parameters from a single MCS for various maintenance plans. In order to fully appreciate the RPI method, a verification procedure was performed. In this study, MC simulations in the orders of several hundred billions were conducted for various flight conditions, material properties, and inspection scheduling, POD and repair/replacement strategies. Since the MC simulations are time-consuming methods, the simulations were conducted parallelly on DoD High Performance Computers (HPC) using a specialized random number generator for parallel computing. The study has shown that RPI method is several orders of magnitude more efficient than traditional Monte Carlo simulations.

Damage Tolerance and Mechanics of Interfaces in Nanostructured Metals

NASA Astrophysics Data System (ADS)

Foley, Daniel J.

The concept of interface driven properties in crystalline metals has been one of the most intensely discussed topics in materials science for decades. Since the 1980s researchers have been exploring the concept of grain boundary engineering as route for tuning properties such as fracture toughness and irradiation resistance. This is especially true in ultra-fine grained and nanocrystalline materials where grain boundary mediated properties become dominant. More recently, materials composed of hierarchical nanostructures, such as amorphous-crystalline nanolaminates, have attracted considerable attention due to their favorable properties, ease of manufacture and highly tunable microstructure. While both grain boundary engineering and hierarchical nanostructures have shown promise there are still questions remaining regarding the role of specific attributes of the microstructure (such as grain boundaries, grain/layer size and inter/intralayer morphology) in determining material properties. This thesis attempts to address these questions by using atomistic simulations to perform deformation and damage loading studies on a series of nanolaminate and bicrystalline structures. During the course of this thesis the roles of layer thickness, interlayer structure and interlayer chemistry on the mechanical properties of Ni-NiX amorphous-crystalline nanolaminates were explored using atomistic simulations. This thesis found that layer thickness/thickness ratio and amorphous layer chemistry play a crucial role in yield strength and Young's modulus. Analysis of the deformation mechanisms at the atomic scale revealed that structures containing single crystalline, crystalline layers undergo plastic deformation when shear transformation zones form in the amorphous layer and impinge on the amorphous-crystalline interface, leading to dislocation emission. However, structures containing nanocrystalline, crystalline layers (both equiaxed and columnar nanocrystalline) undergo plastic deformation through a combination of grain boundary sliding and grain boundary mediated dislocation nucleation. Since grain boundaries were found to play a critical role as sources and sinks for dislocations in amorphous-crystalline nanolaminates a follow-up study on the effect of grain boundary character on damage accumulation/accommodation in copper symmetric tilt grain boundaries was performed. This study found that grain boundaries will become saturated with damage, a state where grain boundary energy and grain boundary free volume oscillate about a plateau during continuous defect loading (vacancy, interstitial and frenkel pair loading were all considered). Further, grain boundary character (specifically equilibrium grain boundary energy) was strongly correlated to the damage accommodation behavior of grain boundaries in copper. Finally, a study that attempted to link grain boundary damage saturation behavior to variations in grain boundary mechanical properties was performed. This study found no direct relationships between grain boundary damage saturation behavior and variations in grain boundary properties. The results of this thesis provide researchers with several strategies for tuning the properties of amorphous-crystalline nanolaminates. These strategies include manipulated bulk attributes such as layer thickness and morphology as well as manipulation of microscale attributes such as grain boundary engineering. Finally, this thesis provides valuable insight into the damage loading/accommodation behavior of FCC symmetric tilt grain boundaries.

An Overview of Innovative Strategies for Fracture Mechanics at NASA Langley Research Center

NASA Technical Reports Server (NTRS)

Ransom, Jonathan B.; Glaessgen, Edward H.; Ratcliffe, James G.

2010-01-01

Engineering fracture mechanics has played a vital role in the development and certification of virtually every aerospace vehicle that has been developed since the mid-20th century. NASA Langley Research Center s Durability, Damage Tolerance and Reliability Branch has contributed to the development and implementation of many fracture mechanics methods aimed at predicting and characterizing damage in both metallic and composite materials. This paper presents a selection of computational, analytical and experimental strategies that have been developed by the branch for assessing damage growth under monotonic and cyclic loading and for characterizing the damage tolerance of aerospace structures

Controlling the self-organizing dynamics in a sandpile model on complex networks by failure tolerance

DOE Office of Scientific and Technical Information (OSTI.GOV)

Qi, Junjian; Pfenninger, Stefan

In this paper, we propose a strategy to control the self-organizing dynamics of the Bak-Tang-Wiesenfeld (BTW) sandpile model on complex networks by allowing some degree of failure tolerance for the nodes and introducing additional active dissipation while taking the risk of possible node damage. We show that the probability for large cascades significantly increases or decreases respectively when the risk for node damage outweighs the active dissipation and when the active dissipation outweighs the risk for node damage. By considering the potential additional risk from node damage, a non-trivial optimal active dissipation control strategy which minimizes the total cost inmore » the system can be obtained. Under some conditions the introduced control strategy can decrease the total cost in the system compared to the uncontrolled model. Moreover, when the probability of damaging a node experiencing failure tolerance is greater than the critical value, then no matter how successful the active dissipation control is, the total cost of the system will have to increase. This critical damage probability can be used as an indicator of the robustness of a network or system. Copyright (C) EPLA, 2015« less

Antioxidant enzymatic activity is linked to waterlogging stress tolerance in citrus.

PubMed

Arbona, Vicent; Hossain, Zahed; López-Climent, María F; Pérez-Clemente, Rosa M; Gómez-Cadenas, Aurelio

2008-04-01

Soil flooding constitutes a seasonal factor that negatively affects plant performance and crop yields. In this work, the relationship between oxidative damage and flooding sensitivity was addressed in three citrus genotypes with different abilities to tolerate waterlogging. We examined leaf visible damage, oxidative damage in terms of malondialdehyde (MDA) concentration, leaf proline concentration, leaf and root ascorbate and glutathione contents and the antioxidant enzyme activities superoxide dismutase (EC 1.15.1.1), ascorbate peroxidase (EC 1.11.1.11), catalase (EC 1.11.1.6) and glutathione reductase (EC 1.8.1.7). No differences in the extent of oxidative damage relative to controls were found among genotypes. However, a different ability to delay the apparition of oxidative damage was associated to a higher tolerance to waterlogging. This ability was linked to an enhanced activated oxygen species' scavenging capacity in terms of an increased antioxidant enzyme activity and higher content in polar antioxidant compounds. Therefore, the existence of a direct relationship between stress sensitivity and the early accumulation of MDA is proposed. In addition, data indicate that the protective role of proline has to be considered minimal as its accumulation was inversely correlated with tolerance to the stress. The positive antioxidant response in Carrizo citrange (Poncirus trifoliata L. Raf. x Citrus sinensis L. Osb.) and Citrumelo CPB 4475 (Poncirus trifoliata L. Raf. x Citrus paradisi L. Macf.) might be responsible for a higher tolerance to flooding stress, whereas in Cleopatra mandarin (Citrus reshni Hort. Ex Tan.), the early accumulation of MDA seems to be associated to an impaired ability for H2O2 scavenging.

Dose dependence of radiation damage in nano-structured amorphous SiOC/crystalline Fe composite

DOE PAGES

Su, Qing; Price, Lloyd; Shao, Lin; ...

2015-10-29

Here, through examination of radiation tolerance properties of amorphous silicon oxycarbide (SiOC) and crystalline Fe composite to averaged damage levels, from approximately 8 to 30 displacements per atom (dpa), we demonstrated that the Fe/SiOC interface and the Fe/amorphous Fe xSi yO z interface act as efficient defect sinks and promote the recombination of vacancies and interstitials. For thick Fe/SiOC multilayers, a clear Fe/SiOC interface remained and no irradiation-induced mixing was observed even after 32 dpa. For thin Fe/SiOC multilayers, an amorphous Fe xSi yO z intermixed layer was observed to form at 8 dpa, but no further layer growth wasmore » observed for higher dpa levels.« less

Aldehyde dehydrogenase 2 protects human umbilical vein endothelial cells against oxidative damage and increases endothelial nitric oxide production to reverse nitroglycerin tolerance.

PubMed

Hu, X Y; Fang, Q; Ma, D; Jiang, L; Yang, Y; Sun, J; Yang, C; Wang, J S

2016-06-10

Medical nitroglycerin (glyceryl trinitrate, GTN) use is limited principally by tolerance typified by a decrease in nitric oxide (NO) produced by biotransformation. Such tolerance may lead to endothelial dysfunction by inducing oxidative stress. In vivo studies have demonstrated that aldehyde dehydrogenase 2 (ALDH2) plays important roles in GTN biotransformation and tolerance. Thus, modification of ALDH2 expression represents a potentially effective strategy to prevent and reverse GTN tolerance and endothelial dysfunction. In this study, a eukaryotic expression vector containing the ALDH2 gene was introduced into human umbilical vein endothelial cells (HUVECs) by liposome-mediated transfection. An indirect immunofluorescence assay showed that ALDH2 expression increased 24 h after transfection. Moreover, real-time polymerase chain reaction and western blotting revealed significantly higher ALDH2 mRNA and protein expression in the gene-transfected group than in the two control groups. GTN tolerance was induced by treating HUVECs with 10 mM GTN for 16 h + 10 min, which significantly decreased NO levels in control cells, but not in those transfected with ALDH2. Overexpression of ALDH2 increased cell survival against GTN-induced cytotoxicity and conferred protection from oxidative damage resulting from nitrate tolerance, accompanied by decreased production of intracellular reactive oxygen species and reduced expression of heme oxygenase 1. Furthermore, ALDH2 overexpression promoted Akt phosphorylation under GTN tolerance conditions. ALDH2 gene transfection can reverse and prevent tolerance to GTN through its bioactivation and protect against oxidative damage, preventing the development of endothelial dysfunction.

FAA/NASA International Symposium on Advanced Structural Integrity Methods for Airframe Durability and Damage Tolerance

NASA Technical Reports Server (NTRS)

Harris, Charles E. (Editor)

1994-01-01

International technical experts in durability and damage tolerance of metallic airframe structures were assembled to present and discuss recent research findings and the development of advanced design and analysis methods, structural concepts, and advanced materials. The symposium focused on the dissemination of new knowledge and the peer-review of progress on the development of advanced methodologies. Papers were presented on: structural concepts for enhanced durability, damage tolerance, and maintainability; new metallic alloys and processing technology; fatigue crack initiation and small crack effects; fatigue crack growth models; fracture mechanics failure, criteria for ductile materials; structural mechanics methodology for residual strength and life prediction; development of flight load spectra for design and testing; and advanced approaches to resist corrosion and environmentally assisted fatigue.

An Approach to Risk-Based Design Incorporating Damage Tolerance Analyses

NASA Technical Reports Server (NTRS)

Knight, Norman F., Jr.; Glaessgen, Edward H.; Sleight, David W.

2002-01-01

Incorporating risk-based design as an integral part of spacecraft development is becoming more and more common. Assessment of uncertainties associated with design parameters and environmental aspects such as loading provides increased knowledge of the design and its performance. Results of such studies can contribute to mitigating risk through a system-level assessment. Understanding the risk of an event occurring, the probability of its occurrence, and the consequences of its occurrence can lead to robust, reliable designs. This paper describes an approach to risk-based structural design incorporating damage-tolerance analysis. The application of this approach to a candidate Earth-entry vehicle is described. The emphasis of the paper is on describing an approach for establishing damage-tolerant structural response inputs to a system-level probabilistic risk assessment.

Damage Tolerance and Reliability of Turbine Engine Components

NASA Technical Reports Server (NTRS)

Chamis, Christos C.

1999-01-01

A formal method is described to quantify structural damage tolerance and reliability in the presence of multitude of uncertainties in turbine engine components. The method is based at the materials behaviour level where primitive variables with their respective scatters are used to describe the behavior. Computational simulation is then used to propagate those uncertainties to the structural scale where damage tolerance and reliability are usually specified. Several sample cases are described to illustrate the effectiveness, versatility, and maturity of the method. Typical results from these methods demonstrate that the methods are mature and that they can be used for future strategic projections and planning to assure better, cheaper, faster, products for competitive advantages in world markets. These results also indicate that the methods are suitable for predicting remaining life in aging or deteriorating structures.

Damage Tolerance and Reliability of Turbine Engine Components

NASA Technical Reports Server (NTRS)

Chamis, Christos C.

1998-01-01

A formal method is described to quantify structural damage tolerance and reliability in the presence of multitude of uncertainties in turbine engine components. The method is based at the materials behavior level where primitive variables with their respective scatters are used to describe that behavior. Computational simulation is then used to propagate those uncertainties to the structural scale where damage tolerance and reliability are usually specified. Several sample cases are described to illustrate the effectiveness, versatility, and maturity of the method. Typical results from these methods demonstrate that the methods are mature and that they can be used for future strategic projections and planning to assure better, cheaper, faster products for competitive advantages in world markets. These results also indicate that the methods are suitable for predicting remaining life in aging or deteriorating structures.

FAA/NASA International Symposium on Advanced Structural Integrity Methods for Airframe Durability and Damage Tolerance, part 2

NASA Technical Reports Server (NTRS)

Harris, Charles E. (Editor)

1994-01-01

The international technical experts in the areas of durability and damage tolerance of metallic airframe structures were assembled to present and discuss recent research findings and the development of advanced design and analysis methods, structural concepts, and advanced materials. The principal focus of the symposium was on the dissemination of new knowledge and the peer-review of progress on the development of advanced methodologies. Papers were presented on the following topics: structural concepts for enhanced durability, damage tolerance, and maintainability; new metallic alloys and processing technology; fatigue crack initiation and small crack effects; fatigue crack growth models; fracture mechanics failure criteria for ductile materials; structural mechanics methodology for residual strength and life prediction; development of flight load spectra for design and testing; and corrosion resistance.

Teeth: Among Nature's Most Durable Biocomposites

NASA Astrophysics Data System (ADS)

Lawn, Brian R.; Lee, James J.-W.; Chai, Herzl

2010-08-01

This paper addresses the durability of natural teeth from a materials perspective. Teeth are depicted as smart biocomposites, highly resistant to cumulative deformation and fracture. Favorable morphological features of teeth at both macroscopic and microscopic levels contribute to an innate damage tolerance. Damage modes are activated readily within the brittle enamel coat but are contained from spreading catastrophically into the vulnerable tooth interior in sustained occlusal loading. Although tooth enamel contains a multitude of microstructural defects that can act as sources of fracture, substantial overloads are required to drive any developing cracks to ultimate failure—nature's strategy is to contain damage rather than avoid it. Tests on model glass-shell systems simulating the basic elements of the tooth enamel/dentin layer structure help to identify important damage modes. Fracture and deformation mechanics provide a basis for analyzing critical conditions for each mode, in terms of characteristic tooth dimensions and materials properties. Comparative tests on extracted human and animal teeth confirm the validity of the model test approach and point to new research directions. Implications in biomechanics, especially as they relate to dentistry and anthropology, are outlined.

Probabilistic Evaluation of Blade Impact Damage

NASA Technical Reports Server (NTRS)

Chamis, C. C.; Abumeri, G. H.

2003-01-01

The response to high velocity impact of a composite blade is probabilistically evaluated. The evaluation is focused on quantifying probabilistically the effects of uncertainties (scatter) in the variables that describe the impact, the blade make-up (geometry and material), the blade response (displacements, strains, stresses, frequencies), the blade residual strength after impact, and the blade damage tolerance. The results of probabilistic evaluations results are in terms of probability cumulative distribution functions and probabilistic sensitivities. Results show that the blade has relatively low damage tolerance at 0.999 probability of structural failure and substantial at 0.01 probability.

Conference on Helicopter Structures Technology, Moffett Field, Calif., November 16-18, 1977, Proceedings

NASA Technical Reports Server (NTRS)

1978-01-01

Work on advanced concepts for helicopter designs is reported. Emphasis is on use of advanced composites, damage-tolerant design, and load calculations. Topics covered include structural design flight maneuver loads using PDP-10 flight dynamics model, use of 3-D finite element analysis in design of helicopter mechanical components, damage-tolerant design of the YUH-61A main rotor system, survivability of helicopters to rotor blade ballistic damage, development of a multitubular spar composite main rotor blade, and a bearingless main rotor structural design approach using advanced composites.

A native plant competitor mediates the impact of above- and belowground damage on an invasive tree.

PubMed

Carrillo, Juli; Siemann, Evan

2016-10-01

Plant competition may mediate the impacts of herbivory on invasive plant species through effects on plant growth and defense. This may predictably depend on whether herbivory occurs above or below ground and on relative plant competitive ability. We simulated the potential impact of above- or belowground damage by biocontrol agents on the growth of a woody invader (Chinese tallow tree, Triadica sebifera) through artificial herbivory, with or without competition with a native grass, little bluestem (Schizachyrium scoparium). We measured two defense responses of Triadica through quantifying constitutive and induced extrafloral nectar production and tolerance of above- and belowground damage (root and shoot biomass regrowth). We examined genetic variation in plant growth and defense across native (China) and invasive (United States) Triadica populations. Without competition, aboveground damage had a greater impact than belowground damage on Triadica performance, whereas with competition and above- and belowground damage impacted Triadica similarly. Whole plant tolerance to damage below ground was negatively associated with tolerance to grass competitors indicating tradeoffs in the ability to tolerate herbivory vs. compete. Competition reduced investment in defensive extrafloral nectar (EFN) production. Aboveground damage inhibited rather than induced EFN production while belowground plant damage did not impact aboveground nectar production. We found some support for the evolution of increased competitive ability hypothesis for invasive plants as United States plants were larger than native China plants and were more plastic in their response to biotic stressors than China plants (they altered their root to shoot ratios dependent on herbivory and competition treatments). Our results indicate that habitat type and the presence of competitors may be a larger determinant of herbivory impact than feeding mode and suggest that integrated pest management strategies including competitive dynamics of recipient communities should be incorporated into biological control agent evaluation at earlier stages. © 2016 by the Ecological Society of America.

Advanced Composite Armor: In Situ Sensing with Carbon Nanotube Networks for Improved Damage Tolerance

DTIC Science & Technology

2013-08-19

aerogel -spun carbon nanotube fibres under tensile loading, Journal of Materials Chemistry, (02 2012): 0. doi: 10.1039/c2jm15869h 08/10/2012 6.00 Amanda... aerogel state and held together by entanglements and van der Waals interactions. The electrical and mechanical behavior of these fibers is provided in...5.1 ± 1.7 (104) Resistance, R [Ω] 67.5 ± 23.6 Table 1. Material properties of the aerogel -spun carbon nanotube fibers. 9    The fibers

A Comparison of Quasi-Static Indentation and Drop-Weight Impact Testing on Carbon-Epoxy Laminates

NASA Technical Reports Server (NTRS)

Prabhakaran, R.

2001-01-01

The project had two objectives: 1) The primary objective was to characterize damage tolerance of composite materials. To accomplish this, polymer matrix composites were to be subjected to static indentation as well as low-velocity impacts and the results analyzed. 2) A second objective was to investigate the effects of laser shock peening on the damage tolerance of aerospace materials, such as aluminum alloys, in terms of crack nucleation and crack propagation. The impact testing was proposed to be performed using a Dynatup drop tower. The specimens were to be placed over a square opening in a steel platen and impacted with a hemispherical tup. The damage was to be characterized in the laminate specimens. The damage tolerance of aerospace alloys was to be studied by conducting fatigue tests on aluminum alloy specimens with prior shock peening treatment. The crack length was to be monitored by a microscope and the crack propagation rate, da/dN, determined.

Simplification of Fatigue Test Requirements for Damage Tolerance of Composite Interstage Launch Vehicle Hardware

NASA Technical Reports Server (NTRS)

Nettles, A. T.; Hodge, A. J.; Jackson, J. R.

2010-01-01

The issue of fatigue loading of structures composed of composite materials is considered in a requirements document that is currently in place for manned launch vehicles. By taking into account the short life of these parts, coupled with design considerations, it is demonstrated that the necessary coupon level fatigue data collapse to a static case. Data from a literature review of past studies that examined compressive fatigue loading after impact and data generated from this experimental study are presented to support this finding. Damage growth, in the form of infrared thermography, was difficult to detect due to rapid degradation of compressive properties once damage growth initiated. Unrealistically high fatigue amplitudes were needed to fail 5 of 15 specimens before 10,000 cycles were reached. Since a typical vehicle structure, such as the Ares I interstage, only experiences a few cycles near limit load, it is concluded that static compression after impact (CAI) strength data will suffice for most launch vehicle structures.

Mechanisms of DNA damage, repair and mutagenesis

PubMed Central

Chatterjee, Nimrat; Walker, Graham C.

2017-01-01

Living organisms are continuously exposed to a myriad of DNA damaging agents that can impact health and modulate disease-states. However, robust DNA repair and damage-bypass mechanisms faithfully protect the DNA by either removing or tolerating the damage to ensure an overall survival. Deviations in this fine-tuning are known to destabilize cellular metabolic homeostasis, as exemplified in diverse cancers where disruption or deregulation of DNA repair pathways results in genome instability. Because routinely used biological, physical and chemical agents impact human health, testing their genotoxicity and regulating their use have become important. In this introductory review, we will delineate mechanisms of DNA damage and the counteracting repair/tolerance pathways to provide insights into the molecular basis of genotoxicity in cells that lays the foundation for subsequent articles in this issue. PMID:28485537

Tolerance and resistance of invasive and native Eupatorium species to generalist herbivore insects

NASA Astrophysics Data System (ADS)

Wang, Rui-Fang; Feng, Yu-Long

2016-11-01

Invasive plants are exotic species that escape control by native specialist enemies. However, exotic plants may still be attacked by locally occurring generalist enemies, which can influence the dynamics of biological invasions. If invasive plants have greater defensive (resistance and tolerance) capabilities than indigenous plants, they may experience less damage from native herbivores. In the present study, we tested this prediction using the invasive plant Eupatorium adenophorum and two native congeners under simulated defoliation and generalist herbivore insect (Helicoverpa armigera and Spodoptera litura) treatments. E. adenophorum was less susceptible and compensated more quickly to damages in biomass production from both treatments compared to its two congeners, exhibiting greater herbivore tolerance. This strong tolerance to damage was associated with greater resource allocation to aboveground structures, leading to a higher leaf area ratio and a lower root: crown mass ratio than those of its native congeners. E. adenophorum also displayed a higher resistance index (which integrates acid detergent fiber, nitrogen content, carbon/nitrogen ratio, leaf mass per area, toughness, and trichome density) than its two congeners. Thus, H. armigera and S. litura performed poorly on E. adenophorum, with less leaf damage, a lengthened insect developmental duration, and decreased pupating: molting ratios compared to those of the native congeners. Strong tolerance and resistance traits may facilitate the successful invasion of E. adenophorum in China and may decrease the efficacy of leaf-feeding biocontrol agents. Our results highlight both the need for further research on defensive traits and their role in the invasiveness and biological control of exotic plants, and suggest that biocontrol of E. adenophorum in China would require damage to the plant far in excess of current levels.

Damage Tolerance Testing of a NASA TransHab Derivative Woven Inflatable Module

NASA Technical Reports Server (NTRS)

Edgecombe, John; delaFuente, Horacio; Valle, Gerard

2009-01-01

Current options for Lunar habitat architecture include inflatable habitats and airlocks. Inflatable structures can have mass and volume advantages over conventional structures. However, inflatable structures carry different inherent risks and are at a lower Technical Readiness Level (TRL) than more conventional metallic structures. One of the risks associated with inflatable structures is in understanding the tolerance to induced damage. The Damage Tolerance Test (DTT) is designed to study the structural integrity of an expandable structure. TransHab (Figure 1) was an experimental inflatable module developed at the NASA/Johnson Space Center in the 1990 s. The TransHab design was originally envisioned for use in Mars Transits but was also studied as a potential habitat for the International Space Station (ISS). The design of the TransHab module was based on a woven design using an Aramid fabric. Testing of this design demonstrated a high level of predictability and repeatability with analytical predictions of stresses and deflections. Based on JSC s experience with the design and analysis of woven inflatable structures, the Damage Tolerance Test article was designed and fabricated using a woven design. The DTT article was inflated to 45 psig, representing 25% of the ultimate burst pressure, and one of the one-inch wide longitudinal structural members was severed by initiating a Linear Shaped Charge (LSC). Strain gage measurements, at the interface between the expandable elements (straps) and the nonexpandable metallic elements for pre-selected longitudinal straps, were taken throughout pressurization of the module and strap separation. Strain gage measurements show no change in longitudinal strap loading at the bulkhead interface after strap separation indicating loads in the restraint layer were re-distributed local to the damaged area due to the effects of friction under high internal pressure loading. The test completed all primary objectives with better than expected results. This paper will discuss space inflatable structures, damage tolerance analysis, test results, and applicability to the Lunar architecture.

Recent Insight into the Kinetic Mechanisms and Conformational Dynamics of Y-Family DNA Polymerases

PubMed Central

2015-01-01

The kinetic mechanisms by which DNA polymerases catalyze DNA replication and repair have long been areas of active research. Recently discovered Y-family DNA polymerases catalyze the bypass of damaged DNA bases that would otherwise block replicative DNA polymerases and stall replication forks. Unlike DNA polymerases from the five other families, the Y-family DNA polymerases have flexible, solvent-accessible active sites that are able to tolerate various types of damaged template bases and allow for efficient lesion bypass. Their promiscuous active sites, however, also lead to fidelities that are much lower than those observed for other DNA polymerases and give rise to interesting mechanistic properties. Additionally, the Y-family DNA polymerases have several other unique structural features and undergo a set of conformational changes during substrate binding and catalysis different from those observed for replicative DNA polymerases. In recent years, pre-steady-state kinetic methods have been extensively employed to reveal a wealth of information about the catalytic properties of these fascinating noncanonical DNA polymerases. Here, we review many of the recent findings on the kinetic mechanisms of DNA polymerization with undamaged and damaged DNA substrates by the Y-family DNA polymerases, and the conformational dynamics employed by these error-prone enzymes during catalysis. PMID:24716482

Characterization of Uranium Tolerance and Biomineralization Potential of Caulobacter crescentus

NASA Astrophysics Data System (ADS)

Park, D.

2015-12-01

Due to its high toxicity and mobility, U(VI) poses a major environmental threat to ecosystems. The ubiquitous aerobic bacterium Caulobacter cresecentus is an attractive candidate for U(VI) bioremediation because of its ability to survive in low-nutrient environments (5, 6), tolerate high U concentrations and mineralize U(VI) aerobically through the formation of uranyl phosphate (U-Pi) precipitates. Despite these attractive environmental properties, both a systems level understanding of the adaptive response pathways involved in U tolerance and the environmental conditions affecting the biomineralization process and stability of biogenic U-Pi minerals remain limited. By measuring changes in both mRNA and protein expression during exposure to high U levels, we have identified the core stress response pathways involved in U tolerance. Pathways associated with heat shock, lipospolysaccharide biosynthesis and transport, outer membrane lipoprotein transport and outermembrane assembly were highly induced at both the RNA and protein levels. Correspondingly, removal of integral components of proteolysis pathways including clpA, clpS and degP significantly reduced U tolerance under biomineralization conditions. Surprisingly, in contrast to many other heavy metals, U did not cause oxidative stress or DNA damage. Together, these analyses indicate that U predominately targets the outermembrane and causes mis-folding of both cytoplasmic and extracytoplasmic proteins. Efforts are currently underway to characterize the morphological and structural properties of biogenic U-Pi minerals and the environmental factors that influence their production and stability. Preliminary AFM studies suggest that U-Pi minerals formed under biomineralization conditions appear morphologically distinct from those formed abiotically between U(VI) and inorganic phosphate. Additionally, we observed that biomineralization tolerates a wide pH range (pH 6-9). Our long-range goal is the development of a conceptual model of the role of microbes in U cycling under oxidizing conditions across the DOE complex, and ultimately, provide DOE with the scientific basis to support decisions for the remediation of legacy sites.

Test and analysis results for composite transport fuselage and wing structures

NASA Technical Reports Server (NTRS)

Deaton, Jerry W.; Kullerd, Susan M.; Madan, Ram C.; Chen, Victor L.

1992-01-01

Automated tow placement (ATP) and stitching of dry textile composite preforms followed by resin transfer molding (RTM) are being investigated by researchers at NASA LaRC and Douglas Aircraft Company as cost-effective manufacturing processes for obtaining damage tolerant fuselage and wing structures for transport aircraft. The Douglas work is being performed under a NASA contract entitled 'Innovative Composites Aircraft Primary Structures (ICAPS)'. Data are presented in this paper to assess the damage tolerance of ATP and RTM fuselage elements with stitched-on stiffeners from compression tests of impacted three-J-stiffened panels and from stiffener pull-off tests. Data are also presented to assess the damage tolerance of RTM wing elements which had stitched skin and stiffeners from impacted single stiffener and three blade-stiffened compression tests and stiffener pull-off tests.

Rotational 3D printing of damage-tolerant composites with programmable mechanics

PubMed Central

Raney, Jordan R.; Compton, Brett G.; Ober, Thomas J.; Shea, Kristina; Lewis, Jennifer A.

2018-01-01

Natural composites exhibit exceptional mechanical performance that often arises from complex fiber arrangements within continuous matrices. Inspired by these natural systems, we developed a rotational 3D printing method that enables spatially controlled orientation of short fibers in polymer matrices solely by varying the nozzle rotation speed relative to the printing speed. Using this method, we fabricated carbon fiber–epoxy composites composed of volume elements (voxels) with programmably defined fiber arrangements, including adjacent regions with orthogonally and helically oriented fibers that lead to nonuniform strain and failure as well as those with purely helical fiber orientations akin to natural composites that exhibit enhanced damage tolerance. Our approach broadens the design, microstructural complexity, and performance space for fiber-reinforced composites through site-specific optimization of their fiber orientation, strain, failure, and damage tolerance. PMID:29348206

Effects of ionizing radiation on embryos of the tardigrade Milnesium cf. tardigradum at different stages of development.

PubMed

Beltrán-Pardo, Eliana; Jönsson, K Ingemar; Wojcik, Andrzej; Haghdoost, Siamak; Harms-Ringdahl, Mats; Bermúdez-Cruz, Rosa M; Bernal Villegas, Jaime E

2013-01-01

Tardigrades represent one of the most desiccation and radiation tolerant animals on Earth, and several studies have documented their tolerance in the adult stage. Studies on tolerance during embryological stages are rare, but differential effects of desiccation and freezing on different developmental stages have been reported, as well as dose-dependent effect of gamma irradiation on tardigrade embryos. Here, we report a study evaluating the tolerance of eggs from the eutardigrade Milnesium cf. tardigradum to three doses of gamma radiation (50, 200 and 500 Gy) at the early, middle, and late stage of development. We found that embryos of the middle and late developmental stages were tolerant to all doses, while eggs in the early developmental stage were tolerant only to a dose of 50 Gy, and showed a declining survival with higher dose. We also observed a delay in development of irradiated eggs, suggesting that periods of DNA repair might have taken place after irradiation induced damage. The delay was independent of dose for eggs irradiated in the middle and late stage, possibly indicating a fixed developmental schedule for repair after induced damage. These results show that the tolerance to radiation in tardigrade eggs changes in the course of their development. The mechanisms behind this pattern are unknown, but may relate to changes in mitotic activities over the embryogenesis and/or to activation of response mechanisms to damaged DNA in the course of development.

Effects of Ionizing Radiation on Embryos of the Tardigrade Milnesium cf. tardigradum at Different Stages of Development

PubMed Central

Beltrán-Pardo, Eliana; Jönsson, K. Ingemar; Wojcik, Andrzej; Haghdoost, Siamak; Harms-Ringdahl, Mats; Bermúdez-Cruz, Rosa M.; Bernal Villegas, Jaime E.

2013-01-01

Tardigrades represent one of the most desiccation and radiation tolerant animals on Earth, and several studies have documented their tolerance in the adult stage. Studies on tolerance during embryological stages are rare, but differential effects of desiccation and freezing on different developmental stages have been reported, as well as dose-dependent effect of gamma irradiation on tardigrade embryos. Here, we report a study evaluating the tolerance of eggs from the eutardigrade Milnesium cf. tardigradum to three doses of gamma radiation (50, 200 and 500 Gy) at the early, middle, and late stage of development. We found that embryos of the middle and late developmental stages were tolerant to all doses, while eggs in the early developmental stage were tolerant only to a dose of 50 Gy, and showed a declining survival with higher dose. We also observed a delay in development of irradiated eggs, suggesting that periods of DNA repair might have taken place after irradiation induced damage. The delay was independent of dose for eggs irradiated in the middle and late stage, possibly indicating a fixed developmental schedule for repair after induced damage. These results show that the tolerance to radiation in tardigrade eggs changes in the course of their development. The mechanisms behind this pattern are unknown, but may relate to changes in mitotic activities over the embryogenesis and/or to activation of response mechanisms to damaged DNA in the course of development. PMID:24039737

76 FR 74655 - Damage Tolerance and Fatigue Evaluation of Composite Rotorcraft Structures

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-01

... and discrete flaws, and impact or other accidental damage (including the discrete source of the... discrete manufacturing defects or accidental damage, is avoided throughout the operational life or... and discrete flaws, and impact or other accidental damage (including the discrete source of the...

Structural Integrity Evaluation of the Lear Fan 2100 Aircraft

NASA Technical Reports Server (NTRS)

Kan, H. P.; Dyer, T. A.

1996-01-01

An in-situ nondestructive inspection was conducted to detect manufacturing and assembly induced defects in the upper two wing surfaces (skin s) and upper fuselage skin of the Lear Fan 2100 aircraft E009. The effects of the defects, detected during the inspection, on the integrity of the structure was analytically evaluated. A systematic evaluation was also conducted to determine the damage tolerance capability of the upper wing skin against impact threats and assembly induced damage. The upper wing skin was divided into small regions for damage tolerance evaluations. Structural reliability, margin of safety, allowable strains, and allowable damage size were computed. The results indicated that the impact damage threat imposed on composite military aircraft structures is too severe for the Lear Fan 2100 upper wing skin. However, the structural integrity is not significantly degraded by the assembly induced damage for properly assembled structures, such as the E009 aircraft.

Re-Tooling the Agency's Engineering Predictive Practices for Durability and Damage Tolerance

NASA Technical Reports Server (NTRS)

Piascik, Robert S.; Knight, Norman F., Jr.

2017-01-01

Over the past decade, the Agency has placed less emphasis on testing and has increasingly relied on computational methods to assess durability and damage tolerance (D&DT) behavior when evaluating design margins for fracture-critical components. With increased emphasis on computational D&DT methods as the standard practice, it is paramount that capabilities of these methods are understood, the methods are used within their technical limits, and validation by well-designed tests confirms understanding. The D&DT performance of a component is highly dependent on parameters in the neighborhood of the damage. This report discusses D&DT method vulnerabilities.

Recent advancements in mechanical characterisation of 3D woven composites

NASA Astrophysics Data System (ADS)

Saleh, Mohamed Nasr; Soutis, Constantinos

2017-12-01

Three dimensional (3D) woven composites have attracted the interest of academia and industry thanks to their damage tolerance characteristics and automated fabric manufacturing. Although much research has been conducted to investigate their out-of-plane "through thickness" properties, still their in-plane properties are not fully understood and rely on extensive experimentation. To date, the literature lacks an inclusive summary of the mechanical characterisation for 3D woven composites. Therefore, the objective of this paper is to provide a comprehensive review of the available research studies on 3D woven composites mechanical characterisation, with less emphasis on the out-of-plane response, but an in-depth review of the in-plane response "un-notched vs. notched". The paper highlights the knowledge gap in the literature of 3D woven composites, suggesting opportunities for future research in this field and a room for improvement in utilising Non-Destructive Techniques (NDT), such as Digital Image Correlation (DIC), Acoustic Emission (AE) and X-ray Computed Tomography (CT), for observing damage initiation and evolution in 3D woven composites that could be used to calibrate and evaluate analytical and numerical models.

Mus308 Processes Oxygen and Nitrogen Ethylation DNA Damage in Germ Cells of Drosophila

PubMed Central

Díaz-Valdés, Nancy; Comendador, Miguel A.; Sierra, L. María

2010-01-01

The D. melanogaster mus308 gene, highly conserved among higher eukaryotes, is implicated in the repair of cross-links and of O-ethylpyrimidine DNA damage, working in a DNA damage tolerance mechanism. However, despite its relevance, its possible role on the processing of different DNA ethylation damages is not clear. To obtain data on mutation frequency and on mutation spectra in mus308 deficient (mus308−) conditions, the ethylating agent diethyl sulfate (DES) was analysed in postmeiotic male germ cells. These data were compared with those corresponding to mus308 efficient conditions. Our results indicate that Mus308 is necessary for the processing of oxygen and N-ethylation damage, for the survival of fertilized eggs depending on the level of induced DNA damage, and for an influence of the DNA damage neighbouring sequence. These results support the role of mus308 in a tolerance mechanism linked to a translesion synthesis pathway and also to the alternative end-joinig system. PMID:20936147

Contamination avoidance devices for poppettype shutoff valves

NASA Technical Reports Server (NTRS)

Endicott, D. L.

1973-01-01

The determination of the cycle life is reported of the scal closure of a typical poppet-type shutoff valve in an uncontaminated GH2 environment and then compared this component performance with simulated operation with GN2 and LN2 containing controlled amounts of AL2O3 contaminant particles. The original valve design was tested for contamination damage tolerance characteristics under full-flow and cyclic-operating conditions, redesigned to improve the damage tolerance to contaminants, and then retested. The redesigned valve was found to have acceptable tolerance characteristics under all full-flow conditions and cyclic operation with small (25-75 microns) particulate contamination. The tolerance characteristics of the valve under cyclic conditions with large (75-250 microns) particulate contamination was improved but was not found to be completely satisfactory.

Design Manual for Impact Damage Tolerant Aircraft Structure

DTIC Science & Technology

1981-10-01

controlled AAA guns having a very high rate of fire, is a significant threat. The final type of non -exploding military projectile-missile warhead fragments...8217~ AGARD-AG-238 NORTH 4TLANTIC TR!EATY ORGANIZATION ADVISORY GROUP FOR AERGSPACE RESEARCH AND DEVELOPMENT (ORGANISATION DU TRAITE DE L’ATLANTIQUE NORD...within the Working Group on Impact Damage Tolerance of Structures and also at the Specialists’ Meeting held in Ankara in September 1975 (see AGARD

Damage Tolerance and Reliability of Turbine Engine Components

NASA Technical Reports Server (NTRS)

Chamis, Christos C.

1999-01-01

This report describes a formal method to quantify structural damage tolerance and reliability in the presence of a multitude of uncertainties in turbine engine components. The method is based at the material behavior level where primitive variables with their respective scatter ranges are used to describe behavior. Computational simulation is then used to propagate the uncertainties to the structural scale where damage tolerance and reliability are usually specified. Several sample cases are described to illustrate the effectiveness, versatility, and maturity of the method. Typical results from this method demonstrate that it is mature and that it can be used to probabilistically evaluate turbine engine structural components. It may be inferred from the results that the method is suitable for probabilistically predicting the remaining life in aging or deteriorating structures, for making strategic projections and plans, and for achieving better, cheaper, faster products that give competitive advantages in world markets.

A damage-tolerant glass.

PubMed

Demetriou, Marios D; Launey, Maximilien E; Garrett, Glenn; Schramm, Joseph P; Hofmann, Douglas C; Johnson, William L; Ritchie, Robert O

2011-02-01

Owing to a lack of microstructure, glassy materials are inherently strong but brittle, and often demonstrate extreme sensitivity to flaws. Accordingly, their macroscopic failure is often not initiated by plastic yielding, and almost always terminated by brittle fracture. Unlike conventional brittle glasses, metallic glasses are generally capable of limited plastic yielding by shear-band sliding in the presence of a flaw, and thus exhibit toughness-strength relationships that lie between those of brittle ceramics and marginally tough metals. Here, a bulk glassy palladium alloy is introduced, demonstrating an unusual capacity for shielding an opening crack accommodated by an extensive shear-band sliding process, which promotes a fracture toughness comparable to those of the toughest materials known. This result demonstrates that the combination of toughness and strength (that is, damage tolerance) accessible to amorphous materials extends beyond the benchmark ranges established by the toughest and strongest materials known, thereby pushing the envelope of damage tolerance accessible to a structural metal.

Damage-tolerant nanotwinned metals with nanovoids under radiation environments

PubMed Central

Chen, Y.; Yu, K Y.; Liu, Y.; Shao, S.; Wang, H.; Kirk, M. A.; Wang, J.; Zhang, X.

2015-01-01

Material performance in extreme radiation environments is central to the design of future nuclear reactors. Radiation induces significant damage in the form of dislocation loops and voids in irradiated materials, and continuous radiation often leads to void growth and subsequent void swelling in metals with low stacking fault energy. Here we show that by using in situ heavy ion irradiation in a transmission electron microscope, pre-introduced nanovoids in nanotwinned Cu efficiently absorb radiation-induced defects accompanied by gradual elimination of nanovoids, enhancing radiation tolerance of Cu. In situ studies and atomistic simulations reveal that such remarkable self-healing capability stems from high density of coherent and incoherent twin boundaries that rapidly capture and transport point defects and dislocation loops to nanovoids, which act as storage bins for interstitial loops. This study describes a counterintuitive yet significant concept: deliberate introduction of nanovoids in conjunction with nanotwins enables unprecedented damage tolerance in metallic materials. PMID:25906997

Damage-tolerant nanotwinned metals with nanovoids under radiation environments.

PubMed

Chen, Y; Yu, K Y; Liu, Y; Shao, S; Wang, H; Kirk, M A; Wang, J; Zhang, X

2015-04-24

Material performance in extreme radiation environments is central to the design of future nuclear reactors. Radiation induces significant damage in the form of dislocation loops and voids in irradiated materials, and continuous radiation often leads to void growth and subsequent void swelling in metals with low stacking fault energy. Here we show that by using in situ heavy ion irradiation in a transmission electron microscope, pre-introduced nanovoids in nanotwinned Cu efficiently absorb radiation-induced defects accompanied by gradual elimination of nanovoids, enhancing radiation tolerance of Cu. In situ studies and atomistic simulations reveal that such remarkable self-healing capability stems from high density of coherent and incoherent twin boundaries that rapidly capture and transport point defects and dislocation loops to nanovoids, which act as storage bins for interstitial loops. This study describes a counterintuitive yet significant concept: deliberate introduction of nanovoids in conjunction with nanotwins enables unprecedented damage tolerance in metallic materials.

Damage-tolerant nanotwinned metals with nanovoids under radiation environments

DOE PAGES

Chen, Y.; Yu, K. Y.; Liu, Y.; ...

2015-04-24

Material performance in extreme radiation environments is central to the design of future nuclear reactors. Radiation induces significant damage in the form of dislocation loops and voids in irradiated materials, and continuous radiation often leads to void growth and subsequent void swelling in metals with low stacking fault energy. Here we show that by using in situ heavy ion irradiation in a transmission electron microscope, pre-introduced nanovoids in nanotwinned Cu efficiently absorb radiation-induced defects accompanied by gradual elimination of nanovoids, enhancing radiation tolerance of Cu. In situ studies and atomistic simulations reveal that such remarkable self-healing capability stems from highmore » density of coherent and incoherent twin boundaries that rapidly capture and transport point defects and dislocation loops to nanovoids, which act as storage bins for interstitial loops. This study describes a counterintuitive yet significant concept: deliberate introduction of nanovoids in conjunction with nanotwins enables unprecedented damage tolerance in metallic materials.« less

The research and development of damage tolerant carbon fiber composites

NASA Astrophysics Data System (ADS)

Miranda, John Armando

This record of study takes a first hand look at corporate research and development efforts to improve the damage tolerance of two unique composite materials used in high performance aerospace applications. The professional internship with The Dow Chemical Company---Dow/United Technologies joint venture describes the intern's involvement in developing patentable process technologies for interleave toughening of high temperature resins and their composites. The subsequent internship with Hexcel Corporation describes the intern's involvement in developing the damage tolerance of novel and existing honeycomb sandwich structure technologies. Through the Doctor of Engineering professional internship experience this student exercised fundamental academic understanding and methods toward accomplishing the corporate objectives of the internship sponsors in a resource efficient and cost-effective manner. Also, the student gained tremendous autonomy through exceptional training in working in focused team environments with highly trained engineers and scientists in achieving important corporate objectives.

Rotational 3D printing of damage-tolerant composites with programmable mechanics.

PubMed

Raney, Jordan R; Compton, Brett G; Mueller, Jochen; Ober, Thomas J; Shea, Kristina; Lewis, Jennifer A

2018-02-06

Natural composites exhibit exceptional mechanical performance that often arises from complex fiber arrangements within continuous matrices. Inspired by these natural systems, we developed a rotational 3D printing method that enables spatially controlled orientation of short fibers in polymer matrices solely by varying the nozzle rotation speed relative to the printing speed. Using this method, we fabricated carbon fiber-epoxy composites composed of volume elements (voxels) with programmably defined fiber arrangements, including adjacent regions with orthogonally and helically oriented fibers that lead to nonuniform strain and failure as well as those with purely helical fiber orientations akin to natural composites that exhibit enhanced damage tolerance. Our approach broadens the design, microstructural complexity, and performance space for fiber-reinforced composites through site-specific optimization of their fiber orientation, strain, failure, and damage tolerance. Copyright © 2018 the Author(s). Published by PNAS.

7 CFR 51.2557 - Tolerances.

Code of Federal Regulations, 2010 CFR

2010-01-01

...) Damage 2.0 2.5 3.0 (b) Serious Damage (Minor Insect or Vertebrate Injury, Mold, Rancid, Decay) 1.5 2.0 2.5 (1) Insect Damage, included in (b) .3 .4 .5 (c) Foreign Material .03 .05 .1 [55 FR 28747, July 13...

7 CFR 51.2557 - Tolerances.

Code of Federal Regulations, 2011 CFR

2011-01-01

...) Damage 2.0 2.5 3.0 (b) Serious Damage (Minor Insect or Vertebrate Injury, Mold, Rancid, Decay) 1.5 2.0 2.5 (1) Insect Damage, included in (b) .3 .4 .5 (c) Foreign Material .03 .05 .1 [55 FR 28747, July 13...

7 CFR 51.307 - Application of tolerances.

Code of Federal Regulations, 2010 CFR

2010-01-01

... STANDARDS) United States Standards for Grades of Apples Application of Tolerances § 51.307 Application of... least one apple which is seriously damaged by insects or affected by decay or internal breakdown may be... have more than 3 times the tolerance specified, except that at least three defective apples may be...

7 CFR 51.307 - Application of tolerances.

Code of Federal Regulations, 2011 CFR

2011-01-01

... STANDARDS) United States Standards for Grades of Apples Application of Tolerances § 51.307 Application of... least one apple which is seriously damaged by insects or affected by decay or internal breakdown may be... have more than 3 times the tolerance specified, except that at least three defective apples may be...

7 CFR 51.307 - Application of tolerances.

Code of Federal Regulations, 2012 CFR

2012-01-01

... STANDARDS) United States Standards for Grades of Apples Application of Tolerances § 51.307 Application of... least one apple which is seriously damaged by insects or affected by decay or internal breakdown may be... have more than 3 times the tolerance specified, except that at least three defective apples may be...

A novel two-step method for screening shade tolerant mutant plants via dwarfism

USDA-ARS?s Scientific Manuscript database

When subjected to shade, plants undergo rapid shoot elongation, which often makes them more prone to disease and mechanical damage. It has been reported that, in turfgrass, induced dwarfism can enhance shade tolerance. Here, we describe a two-step procedure for isolating shade tolerant mutants of ...

Constraints on the evolution of tolerance to herbicide in the common morning glory: resistance and tolerance are mutually exclusive.

PubMed

Baucom, Regina S; Mauricio, Rodney

2008-11-01

Evolutionary biologists explain the maintenance of intermediate levels of defense in plant populations as being due to trade-offs, or negative genetic covariances among ecologically important traits. Attempts at detecting trade-offs as constraints on the evolution of defense have not always been successful, leading some to conclude that such trade-offs rarely explain current levels of defense in the population. Using the agricultural pest Ipomoea purpurea, we measured correlations between traits involved in defense to glyphosate, the active ingredient in Roundup, a widely used herbicide. We found significant allocation costs of tolerance, as well as trade-offs between resistance and two measures of tolerance to glyphosate. Selection on resistance and tolerance exhibited differing patterns: tolerance to leaf damage was under negative directional selection, whereas resistance was under positive directional selection. The joint pattern of selection on resistance and tolerance to leaf damage indicated the presence of alternate peaks in the fitness landscape such that a combination of either high tolerance and low resistance, or high resistance and low tolerance was favored. The widespread use of this herbicide suggests that it is likely an important selective agent on weed populations. Understanding the evolutionary dynamics of herbicide defense traits is thus of increasing importance in the context of human-mediated evolution.

Investigation of low-velocity impact damage in fibre-metal-laminates

NASA Astrophysics Data System (ADS)

Laliberte, Jeremy F.

2002-04-01

Fibre-metal-laminates (FMLs) represent a significant evolution in airframe material technology. This new family of materials combines low density, high strength and excellent damage tolerance through the use of metal layers strengthened with fibre-reinforced polymer layers. When subjected to low-velocity impact these laminates like traditional composites, develop internal delamination damage, matrix cracks and limited fibre fractures. Also, as in traditional composites, this damage is hidden within the laminate. A method for predicting the amount of internal damage would reduce the experimental testing requirements for the certification of new laminates. This thesis describes the development of a modelling methodology that makes use of a new material subroutine based on continuum damage mechanics in the explicit finite-element code LS-DYNA. This subroutine was verified using the experimental data from low-velocity impact tests of various types of GLARE (GLAss REinforced) aluminum laminates, a common type of commercially available fibre-metal-laminate. Static characterization tests were also conducted on GLARE coupons to provide basic property data for the development of the model. These included static tensile tests and double cantilever beam delamination tests. The modelling methodology was used to improve simulations of low-velocity impact on GLARE laminates. The simulations demonstrated that intralaminar damage has a greater effect on the impact response of the panels than interlaminar damage. Parts of this thesis were components of a multi-year collaborative FML Durability Project between Carleton University, Bombardier Aerospace and the National Research Council Canada.

Radiation endurance in Al2O3 nanoceramics

NASA Astrophysics Data System (ADS)

García Ferré, F.; Mairov, A.; Ceseracciu, L.; Serruys, Y.; Trocellier, P.; Baumier, C.; Kaïtasov, O.; Brescia, R.; Gastaldi, D.; Vena, P.; Beghi, M. G.; Beck, L.; Sridharan, K.; di Fonzo, F.

2016-09-01

The lack of suitable materials solutions stands as a major challenge for the development of advanced nuclear systems. Most issues are related to the simultaneous action of high temperatures, corrosive environments and radiation damage. Oxide nanoceramics are a promising class of materials which may benefit from the radiation tolerance of nanomaterials and the chemical compatibility of ceramics with many highly corrosive environments. Here, using thin films as a model system, we provide new insights into the radiation tolerance of oxide nanoceramics exposed to increasing damage levels at 600 °C -namely 20, 40 and 150 displacements per atom. Specifically, we investigate the evolution of the structural features, the mechanical properties, and the response to impact loading of Al2O3 thin films. Initially, the thin films contain a homogeneous dispersion of nanocrystals in an amorphous matrix. Irradiation induces crystallization of the amorphous phase, followed by grain growth. Crystallization brings along an enhancement of hardness, while grain growth induces softening according to the Hall-Petch effect. During grain growth, the excess mechanical energy is dissipated by twinning. The main energy dissipation mechanisms available upon impact loading are lattice plasticity and localized amorphization. These mechanisms are available in the irradiated material, but not in the as-deposited films.

Mechanisms of radiation-induced segregation in CrFeCoNi-based single-phase concentrated solid solution alloys

DOE PAGES

He, Mo-Rigen; Wang, Shuai; Shi, Shi; ...

2016-12-31

Single-phase concentrated solid solution alloys have attracted wide interest due to their superior mechanical properties and enhanced radiation tolerance, which make them promising candidates for the structural applications in next-generation nuclear reactors. However, little has been understood about the intrinsic stability of their as-synthesized, high-entropy configurations against radiation damage. In this paper, we report the element segregation in CrFeCoNi, CrFeCoNiMn, and CrFeCoNiPd equiatomic alloys when subjected to 1250 kV electron irradiations at 400 °C up to a damage level of 1 displacement per atom. Cr/Fe/Mn/Pd can deplete and Co/Ni can accumulate at radiation-induced dislocation loops, while the actively segregating elementsmore » are alloy-specific. Moreover, electron-irradiated matrix of CrFeCoNiMn and CrFeCoNiPd shows L1 0 (NiMn)-type ordering decomposition and <001>-oriented spinodal decomposition between Co/Ni and Pd, respectively. Finally, these findings are rationalized based on the atomic size difference and enthalpy of mixing between the alloying elements, and identify a new important requirement to the design of radiation-tolerant alloys through modification of the composition.« less

Radiation endurance in Al2O3 nanoceramics

PubMed Central

García Ferré, F.; Mairov, A.; Ceseracciu, L.; Serruys, Y.; Trocellier, P.; Baumier, C.; Kaïtasov, O.; Brescia, R.; Gastaldi, D.; Vena, P.; Beghi, M. G.; Beck, L.; Sridharan, K.; Di Fonzo, F.

2016-01-01

The lack of suitable materials solutions stands as a major challenge for the development of advanced nuclear systems. Most issues are related to the simultaneous action of high temperatures, corrosive environments and radiation damage. Oxide nanoceramics are a promising class of materials which may benefit from the radiation tolerance of nanomaterials and the chemical compatibility of ceramics with many highly corrosive environments. Here, using thin films as a model system, we provide new insights into the radiation tolerance of oxide nanoceramics exposed to increasing damage levels at 600 °C –namely 20, 40 and 150 displacements per atom. Specifically, we investigate the evolution of the structural features, the mechanical properties, and the response to impact loading of Al2O3 thin films. Initially, the thin films contain a homogeneous dispersion of nanocrystals in an amorphous matrix. Irradiation induces crystallization of the amorphous phase, followed by grain growth. Crystallization brings along an enhancement of hardness, while grain growth induces softening according to the Hall-Petch effect. During grain growth, the excess mechanical energy is dissipated by twinning. The main energy dissipation mechanisms available upon impact loading are lattice plasticity and localized amorphization. These mechanisms are available in the irradiated material, but not in the as-deposited films. PMID:27653832

Fuel containment and damage tolerance in large composite primary aircraft structures. Phase 2: Testing

NASA Technical Reports Server (NTRS)

Sandifer, J. P.; Denny, A.; Wood, M. A.

1985-01-01

Technical issues associated with fuel containment and damage tolerance of composite wing structures for transport aircraft were investigated. Material evaluation tests were conducted on two toughened resin composites: Celion/HX1504 and Celion/5245. These consisted of impact, tension, compression, edge delamination, and double cantilever beam tests. Another test series was conducted on graphite/epoxy box beams simulating a wing cover to spar cap joint configuration of a pressurized fuel tank. These tests evaluated the effectiveness of sealing methods with various fastener types and spacings under fatigue loading and with pressurized fuel. Another test series evaluated the ability of the selected coatings, film, and materials to prevent fuel leakage through 32-ply AS4/2220-1 laminates at various impact energy levels. To verify the structural integrity of the technology demonstration article structural details, tests were conducted on blade stiffened panels and sections. Compression tests were performed on undamaged and impacted stiffened AS4/2220-1 panels and smaller element tests to evaluate stiffener pull-off, side load and failsafe properties. Compression tests were also performed on panels subjected to Zone 2 lightning strikes. All of these data were integrated into a demonstration article representing a moderately loaded area of a transport wing. This test combined lightning strike, pressurized fuel, impact, impact repair, fatigue and residual strength.

Damage tolerance protein Mus81 associates with the FHA1 domain of checkpoint kinase Cds1.

PubMed

Boddy, M N; Lopez-Girona, A; Shanahan, P; Interthal, H; Heyer, W D; Russell, P

2000-12-01

Cds1, a serine/threonine kinase, enforces the S-M checkpoint in the fission yeast Schizosaccharomyces pombe. Cds1 is required for survival of replicational stress caused by agents that stall replication forks, but how Cds1 performs these functions is largely unknown. Here we report that the forkhead-associated-1 (FHA1) protein-docking domain of Cds1 interacts with Mus81, an evolutionarily conserved damage tolerance protein. Mus81 has an endonuclease homology domain found in the XPF nucleotide excision repair protein. Inactivation of mus81 reveals a unique spectrum of phenotypes. Mus81 enables survival of deoxynucleotide triphosphate starvation, UV radiation, and DNA polymerase impairment. Mus81 is essential in the absence of Bloom's syndrome Rqh1 helicase and is required for productive meiosis. Genetic epistasis studies suggest that Mus81 works with recombination enzymes to properly replicate damaged DNA. Inactivation of Mus81 triggers a checkpoint-dependent delay of mitosis. We propose that Mus81 is involved in the recruitment of Cds1 to aberrant DNA structures where Cds1 modulates the activity of damage tolerance enzymes.

Detecting damage in full-scale honeycomb sandwich composite curved fuselage panels through frequency response

NASA Astrophysics Data System (ADS)

Leone, Frank A., Jr.; Ozevin, Didem; Mosinyi, Bao; Bakuckas, John G., Jr.; Awerbuch, Jonathan; Lau, Alan; Tan, Tein-Min

2008-03-01

Preliminary tests were conducted using frequency response (FR) characteristics to determine damage initiation and growth in a honeycomb sandwich graphite/epoxy curved panel. This investigation was part of a more general study investigating the damage tolerance characteristics of several such panels subjected to quasi-static internal pressurization combined with hoop and axial loading. The panels were tested at the Full-Scale Aircraft Structural Test Evaluation and Research (FASTER) facility located at the Federal Aviation Administration William J. Hughes Technical Center in Atlantic City, NJ. The overall program objective was to investigate the damage tolerance characteristics of full-scale composite curved aircraft fuselage panels and the evolution of damage under quasi-static loading up to failure. This paper focuses on one aspect of this comprehensive investigation: the effect of state-of-damage on the characteristics of the frequency response of the subject material. The results presented herein show that recording the frequency response could be used for real-time monitoring of damage growth and in determining damage severity in full-scale composites fuselage aircraft structures.

E-Beam Processing of Polymer Matrix Composites for Multifunctional Radiation Shielding

NASA Technical Reports Server (NTRS)

Hou, Tan-Hung; Wilson, John W.; Jensen, Brian J.; Thibeault, Sheila A.; Chang, Chie K.; Kiefer, Richard L.

2005-01-01

Aliphatic polymers were identified as optimum radiation shielding polymeric materials for building multifunctional structural elements for in-space habitats. Conceptual damage tolerant configurations of polyolefins have been proposed, but many manufacturing issues relied on methods and materials which have sub-optimal radiation shielding characteristics (for example, epoxy matrix and adhesives). In the present approach, we shall investigate e-beam processing technologies for inclusion of high-strength aliphatic polymer reinforcement structures into a highly cross-linked polyolefin matrix. This paper reports the baseline thermo-mechanical properties of low density polyethylene and highly crystallized polyethylene.

Textile composite fuselage structures development

NASA Technical Reports Server (NTRS)

Jackson, Anthony C.; Barrie, Ronald E.; Chu, Robert L.

1993-01-01

Phase 2 of the NASA ACT Contract (NAS1-18888), Advanced Composite Structural Concepts and Materials Technology for Transport Aircraft Structures, focuses on textile technology, with resin transfer molding or powder coated tows. The use of textiles has the potential for improving damage tolerance, reducing cost and saving weight. This program investigates resin transfer molding (RTM), as a maturing technology for high fiber volume primary structures and powder coated tows as an emerging technology with a high potential for significant cost savings and superior structural properties. Powder coated tow technology has promise for significantly improving the processibility of high temperature resins such as polyimides.

Computational predictive methods for fracture and fatigue

NASA Technical Reports Server (NTRS)

Cordes, J.; Chang, A. T.; Nelson, N.; Kim, Y.

1994-01-01

The damage-tolerant design philosophy as used by aircraft industries enables aircraft components and aircraft structures to operate safely with minor damage, small cracks, and flaws. Maintenance and inspection procedures insure that damages developed during service remain below design values. When damage is found, repairs or design modifications are implemented and flight is resumed. Design and redesign guidelines, such as military specifications MIL-A-83444, have successfully reduced the incidence of damage and cracks. However, fatigue cracks continue to appear in aircraft well before the design life has expired. The F16 airplane, for instance, developed small cracks in the engine mount, wing support, bulk heads, the fuselage upper skin, the fuel shelf joints, and along the upper wings. Some cracks were found after 600 hours of the 8000 hour design service life and design modifications were required. Tests on the F16 plane showed that the design loading conditions were close to the predicted loading conditions. Improvements to analytic methods for predicting fatigue crack growth adjacent to holes, when multiple damage sites are present, and in corrosive environments would result in more cost-effective designs, fewer repairs, and fewer redesigns. The overall objective of the research described in this paper is to develop, verify, and extend the computational efficiency of analysis procedures necessary for damage tolerant design. This paper describes an elastic/plastic fracture method and an associated fatigue analysis method for damage tolerant design. Both methods are unique in that material parameters such as fracture toughness, R-curve data, and fatigue constants are not required. The methods are implemented with a general-purpose finite element package. Several proof-of-concept examples are given. With further development, the methods could be extended for analysis of multi-site damage, creep-fatigue, and corrosion fatigue problems.

Computational predictive methods for fracture and fatigue

NASA Astrophysics Data System (ADS)

Cordes, J.; Chang, A. T.; Nelson, N.; Kim, Y.

1994-09-01

The damage-tolerant design philosophy as used by aircraft industries enables aircraft components and aircraft structures to operate safely with minor damage, small cracks, and flaws. Maintenance and inspection procedures insure that damages developed during service remain below design values. When damage is found, repairs or design modifications are implemented and flight is resumed. Design and redesign guidelines, such as military specifications MIL-A-83444, have successfully reduced the incidence of damage and cracks. However, fatigue cracks continue to appear in aircraft well before the design life has expired. The F16 airplane, for instance, developed small cracks in the engine mount, wing support, bulk heads, the fuselage upper skin, the fuel shelf joints, and along the upper wings. Some cracks were found after 600 hours of the 8000 hour design service life and design modifications were required. Tests on the F16 plane showed that the design loading conditions were close to the predicted loading conditions. Improvements to analytic methods for predicting fatigue crack growth adjacent to holes, when multiple damage sites are present, and in corrosive environments would result in more cost-effective designs, fewer repairs, and fewer redesigns. The overall objective of the research described in this paper is to develop, verify, and extend the computational efficiency of analysis procedures necessary for damage tolerant design. This paper describes an elastic/plastic fracture method and an associated fatigue analysis method for damage tolerant design. Both methods are unique in that material parameters such as fracture toughness, R-curve data, and fatigue constants are not required. The methods are implemented with a general-purpose finite element package. Several proof-of-concept examples are given. With further development, the methods could be extended for analysis of multi-site damage, creep-fatigue, and corrosion fatigue problems.

Aligned composite structures for mitigation of impact damage and resistance to wear in dynamic environments

DOEpatents

Mulligan, Anthony C.; Rigali, Mark J.; Sutaria, Manish P.; Popovich, Dragan; Halloran, Joseph P.; Fulcher, Michael L.; Cook, Randy C.

2005-12-13

Fibrous monolith composites having architectures that provide increased flaw insensitivity, improved hardness, wear resistance and damage tolerance and methods of manufacture thereof are provided for use in dynamic environments to mitigate impact damage and increase wear resistance.

Aligned composite structures for mitigation of impact damage and resistance to wear in dynamic environments

DOEpatents

Mulligan, Anthony C.; Rigali, Mark J.; Sutaria, Manish P.; Popovich, Dragan; Halloran, Joseph P.; Fulcher, Michael L.; Cook, Randy C.

2009-04-14

Fibrous monolith composites having architectures that provide increased flaw insensitivity, improved hardness, wear resistance and damage tolerance and methods of manufacture thereof are provided for use in dynamic environments to mitigate impact damage and increase wear resistance.

Aligned composite structures for mitigation of impact damage and resistance to wear in dynamic environments

DOEpatents

Rigali, Mark J.; Sutaria, Manish P.; Mulligan, Anthony C.; Popovich, Dragan

2004-03-23

Fibrous monolith composites having architectures that provide increased flaw insensitivity, improved hardness, wear resistance and damage tolerance and methods of manufacture thereof are provided for use in dynamic environments to mitigate impact damage and increase wear resistance.

Radiation-induced amorphization resistance and radiation tolerance in structurally related oxides.

PubMed

Sickafus, Kurt E; Grimes, Robin W; Valdez, James A; Cleave, Antony; Tang, Ming; Ishimaru, Manabu; Corish, Siobhan M; Stanek, Christopher R; Uberuaga, Blas P

2007-03-01

Ceramics destined for use in hostile environments such as nuclear reactors or waste immobilization must be highly durable and especially resistant to radiation damage effects. In particular, they must not be prone to amorphization or swelling. Few ceramics meet these criteria and much work has been devoted in recent years to identifying radiation-tolerant ceramics and the characteristics that promote radiation tolerance. Here, we examine trends in radiation damage behaviour for families of compounds related by crystal structure. Specifically, we consider oxides with structures related to the fluorite crystal structure. We demonstrate that improved amorphization resistance characteristics are to be found in compounds that have a natural tendency to accommodate lattice disorder.

Microtubules self-repair in response to mechanical stress

PubMed Central

Schaedel, Laura; John, Karin; Gaillard, Jérémie; Nachury, Maxence V.; Blanchoin, Laurent; Théry, Manuel

2015-01-01

Microtubules - which define the shape of axons, cilia and flagella, and provide tracks for intracellular transport - can be highly bent by intracellular forces, and microtubule structure and stiffness are thought to be affected by physical constraints. Yet how microtubules tolerate the vast forces exerted on them remains unknown. Here, by using a microfluidic device, we show that microtubule stiffness decreases incrementally with each cycle of bending and release. Similar to other cases of material fatigue, the concentration of mechanical stresses on pre-existing defects in the microtubule lattice is responsible for the generation of larger damages, which further decrease microtubule stiffness. Strikingly, damaged microtubules were able to incorporate new tubulin dimers into their lattice and recover their initial stiffness. Our findings demonstrate that microtubules are ductile materials with self-healing properties, that their dynamics does not exclusively occur at their ends, and that their lattice plasticity enables the microtubules' adaptation to mechanical stresses. PMID:26343914

Microtubules self-repair in response to mechanical stress

NASA Astrophysics Data System (ADS)

Schaedel, Laura; John, Karin; Gaillard, Jérémie; Nachury, Maxence V.; Blanchoin, Laurent; Théry, Manuel

2015-11-01

Microtubules--which define the shape of axons, cilia and flagella, and provide tracks for intracellular transport--can be highly bent by intracellular forces, and microtubule structure and stiffness are thought to be affected by physical constraints. Yet how microtubules tolerate the vast forces exerted on them remains unknown. Here, by using a microfluidic device, we show that microtubule stiffness decreases incrementally with each cycle of bending and release. Similar to other cases of material fatigue, the concentration of mechanical stresses on pre-existing defects in the microtubule lattice is responsible for the generation of more extensive damage, which further decreases microtubule stiffness. Strikingly, damaged microtubules were able to incorporate new tubulin dimers into their lattice and recover their initial stiffness. Our findings demonstrate that microtubules are ductile materials with self-healing properties, that their dynamics does not exclusively occur at their ends, and that their lattice plasticity enables the microtubules' adaptation to mechanical stresses.

Microtubules self-repair in response to mechanical stress.

PubMed

Schaedel, Laura; John, Karin; Gaillard, Jérémie; Nachury, Maxence V; Blanchoin, Laurent; Théry, Manuel

2015-11-01

Microtubules--which define the shape of axons, cilia and flagella, and provide tracks for intracellular transport--can be highly bent by intracellular forces, and microtubule structure and stiffness are thought to be affected by physical constraints. Yet how microtubules tolerate the vast forces exerted on them remains unknown. Here, by using a microfluidic device, we show that microtubule stiffness decreases incrementally with each cycle of bending and release. Similar to other cases of material fatigue, the concentration of mechanical stresses on pre-existing defects in the microtubule lattice is responsible for the generation of more extensive damage, which further decreases microtubule stiffness. Strikingly, damaged microtubules were able to incorporate new tubulin dimers into their lattice and recover their initial stiffness. Our findings demonstrate that microtubules are ductile materials with self-healing properties, that their dynamics does not exclusively occur at their ends, and that their lattice plasticity enables the microtubules' adaptation to mechanical stresses.

Performance of Nanotube-Based Ceramic Composites: Modeling and Experiment

NASA Technical Reports Server (NTRS)

Curtin, W. A.; Sheldon, B. W.; Xu, J.

2004-01-01

The excellent mechanical properties of carbon-nanotubes are driving research into the creation of new strong, tough nanocomposite systems. In this program, our initial work presented the first evidence of toughening mechanisms operating in carbon-nanotube- reinforced ceramic composites using a highly-ordered array of parallel multiwall carbon-nanotubes (CNTs) in an alumina matrix. Nanoindentation introduced controlled cracks and the damage was examined by SEM. These nanocomposites exhibit the three hallmarks of toughening in micron-scale fiber composites: crack deflection at the CNT/matrix interface; crack bridging by CNTs; and CNT pullout on the fracture surfaces. Furthermore, for certain geometries a new mechanism of nanotube collapse in shear bands was found, suggesting that these materials can have multiaxial damage tolerance. The quantitative indentation data and computational models were used to determine the multiwall CNT axial Young's modulus as 200-570 GPa, depending on the nanotube geometry and quality.

7 CFR 51.1405 - Application of tolerances.

Code of Federal Regulations, 2010 CFR

2010-01-01

... STANDARDS) United States Standards for Grades of Pecans in the Shell 1 Application of Tolerances § 51.1405... that at least one pecan which is seriously damaged by live insects inside the shell is permitted...

The Effect of Molecular Weight on the Composite Properties of Cured Phenylethynyl Terminated Imide Oligomers

NASA Technical Reports Server (NTRS)

Smith, J. G., Jr.; Connell, J. W.; Hergenrother, P. M.

1997-01-01

As part of a program to develop high temperature/high performance structural resins for aeronautical applications, imide oligomers containing terminal phenylethynyl groups with calculated number average molecular weights of 1250, 2500 and 5000 g/mol were prepared, characterized, and evaluated as adhesives and composite matrix resins. The goal of this work was to develop resin systems that are processable using conventional processing equipment into void free composites that exhibit high mechanical properties with long term high temperature durability, and are not affected by exposure to common aircraft fluids. The imide oligomers containing terminal phenylethynyl groups were fabricated into titanium adhesive specimens and IM-7 carbon fiber laminates under 0.1 - 1.4 MPa for 1 hr at 350-371 C. The lower molecular weight oligomers exhibited higher cured Tg, better processability, and better retention of mechanical properties at elevated temperature without significantly sacrificing toughness or damage tolerance than the higher molecular weight oligomer. The neat resin, adhesive and composite properties of the cured polymers will be presented.

DNA lesion identity drives choice of damage tolerance pathway in murine cell chromosomes.

PubMed

Cohen, Isadora S; Bar, Carmit; Paz-Elizur, Tamar; Ainbinder, Elena; Leopold, Karoline; de Wind, Niels; Geacintov, Nicholas; Livneh, Zvi

2015-02-18

DNA-damage tolerance (DDT) via translesion DNA synthesis (TLS) or homology-dependent repair (HDR) functions to bypass DNA lesions encountered during replication, and is critical for maintaining genome stability. Here, we present piggyBlock, a new chromosomal assay that, using piggyBac transposition of DNA containing a known lesion, measures the division of labor between the two DDT pathways. We show that in the absence of DNA damage response, tolerance of the most common sunlight-induced DNA lesion, TT-CPD, is achieved by TLS in mouse embryo fibroblasts. Meanwhile, BP-G, a major smoke-induced DNA lesion, is bypassed primarily by HDR, providing the first evidence for this mechanism being the main tolerance pathway for a biologically important lesion in a mammalian genome. We also show that, far from being a last-resort strategy as it is sometimes portrayed, TLS operates alongside nucleotide excision repair, handling 40% of TT-CPDs in repair-proficient cells. Finally, DDT acts in mouse embryonic stem cells, exhibiting the same pattern—mutagenic TLS included—despite the risk of propagating mutations along all cell lineages. The new method highlights the importance of HDR, and provides an effective tool for studying DDT in mammalian cells.

DNA lesion identity drives choice of damage tolerance pathway in murine cell chromosomes

PubMed Central

Cohen, Isadora S.; Bar, Carmit; Paz-Elizur, Tamar; Ainbinder, Elena; Leopold, Karoline; de Wind, Niels; Geacintov, Nicholas; Livneh, Zvi

2015-01-01

DNA-damage tolerance (DDT) via translesion DNA synthesis (TLS) or homology-dependent repair (HDR) functions to bypass DNA lesions encountered during replication, and is critical for maintaining genome stability. Here, we present piggyBlock, a new chromosomal assay that, using piggyBac transposition of DNA containing a known lesion, measures the division of labor between the two DDT pathways. We show that in the absence of DNA damage response, tolerance of the most common sunlight-induced DNA lesion, TT-CPD, is achieved by TLS in mouse embryo fibroblasts. Meanwhile, BP-G, a major smoke-induced DNA lesion, is bypassed primarily by HDR, providing the first evidence for this mechanism being the main tolerance pathway for a biologically important lesion in a mammalian genome. We also show that, far from being a last-resort strategy as it is sometimes portrayed, TLS operates alongside nucleotide excision repair, handling 40% of TT-CPDs in repair-proficient cells. Finally, DDT acts in mouse embryonic stem cells, exhibiting the same pattern—mutagenic TLS included—despite the risk of propagating mutations along all cell lineages. The new method highlights the importance of HDR, and provides an effective tool for studying DDT in mammalian cells. PMID:25589543

Sodium chloride toxicity and the cellular basis of salt tolerance in halophytes

PubMed Central

Flowers, Timothy J.; Munns, Rana; Colmer, Timothy D.

2015-01-01

Background Halophytes are the flora of saline soils. They adjust osmotically to soil salinity by accumulating ions and sequestering the vast majority of these (generally Na+ and Cl−) in vacuoles, while in the cytoplasm organic solutes are accumulated to prevent adverse effects on metabolism. At high salinities, however, growth is inhibited. Possible causes are: toxicity to metabolism of Na+ and/or Cl− in the cytoplasm; insufficient osmotic adjustment resulting in reduced net photosynthesis because of stomatal closure; reduced turgor for expansion growth; adverse cellular water relations if ions build up in the apoplast (cell walls) of leaves; diversion of energy needed to maintain solute homeostasis; sub-optimal levels of K+ (or other mineral nutrients) required for maintaining enzyme activities; possible damage from reactive oxygen species; or changes in hormonal concentrations. Scope This review discusses the evidence for Na+ and Cl− toxicity and the concept of tissue tolerance in relation to halophytes. Conclusions The data reviewed here suggest that halophytes tolerate cytoplasmic Na+ and Cl− concentrations of 100–200 mm, but whether these ions ever reach toxic concentrations that inhibit metabolism in the cytoplasm or cause death is unknown. Measurements of ion concentrations in the cytosol of various cell types for contrasting species and growth conditions are needed. Future work should also focus on the properties of the tonoplast that enable ion accumulation and prevent ion leakage, such as the special properties of ion transporters and of the lipids that determine membrane permeability. PMID:25466549

Design of Composite Structures for Reliability and Damage Tolerance

NASA Technical Reports Server (NTRS)

Rais-Rohani, Masoud

1999-01-01

A summary of research conducted during the first year is presented. The research objectives were sought by conducting two tasks: (1) investigation of probabilistic design techniques for reliability-based design of composite sandwich panels, and (2) examination of strain energy density failure criterion in conjunction with response surface methodology for global-local design of damage tolerant helicopter fuselage structures. This report primarily discusses the efforts surrounding the first task and provides a discussion of some preliminary work involving the second task.

Low cost damage tolerant composite fabrication

NASA Technical Reports Server (NTRS)

Palmer, R. J.; Freeman, W. T.

1988-01-01

The resin transfer molding (RTM) process applied to composite aircraft parts offers the potential for using low cost resin systems with dry graphite fabrics that can be significantly less expensive than prepreg tape fabricated components. Stitched graphite fabric composites have demonstrated compression after impact failure performance that equals or exceeds that of thermoplastic or tough thermoset matrix composites. This paper reviews methods developed to fabricate complex shape composite parts using stitched graphite fabrics to increase damage tolerance with RTM processes to reduce fabrication cost.

Mitochondria inheritance is a key factor for tolerance to dehydration in wine yeast production.

PubMed

Picazo, C; Gamero-Sandemetrio, E; Orozco, H; Albertin, W; Marullo, P; Matallana, E; Aranda, A

2015-03-01

Mitochondria are the cell's powerhouse when organisms are grown in the presence of oxygen. They are also the source of reactive oxygen species that cause damage to the biochemical components of the cell and lead to cellular ageing and death. Under winemaking conditions, Saccharomyces yeasts exclusively have a fermentative metabolism due to the high sugar content of grape must. However, their production as an active dry yeast (ADY) form required aerobic propagation and a dehydration process. In these industrial steps, oxidative stress is particularly harmful for the cell. In this work, we analysed the impact of the mitochondrial genome on oxidative stress response, longevity and dehydration tolerance using the synthetic interspecific hybrids obtained between two S. cerevisiae and S. uvarum strains. The isogenic nature of nuclear DNA of such hybrids allowed us to analyse the impact of mitochondrial DNA for fermentative and oxidative stress conditions. Under grape must conditions, the inheritance of mitochondrial DNA poorly impacted the fermentative performance of interspecific hybrids, unlike the hybrids with S. cerevisiae mitochondrial inheritance, which displayed increased tolerance to oxidative stress and dehydration, and showed an extended chronological longevity when cells were grown with aeration. In modern oenology, yeast starters are employed to inoculate grape juice, usually in the form of active dry yeast (ADY). The dehydration process implies stressful conditions that lead to oxidative damage. Other yeast species and interspecific hybrids other than Saccharomyces cerevisiae may be used to confer novel properties to the final product. However, these yeasts are usually more sensitive to drying. Understanding the causes of oxidative stress tolerance is therefore necessary for developing the use of these organisms in industry. This study indicates the impact of mitochondrial DNA inheritance for oxidative stress resistance in an interspecific context using isogenic Saccharomyces cerevisiae × Saccharomyces uvarum hybrids. © 2014 The Society for Applied Microbiology.

Theoretical investigation on radiation tolerance of {{\\boldsymbol{M}}}_{{\\boldsymbol{n}}+1}{{\\boldsymbol{AX}}}_{{\\boldsymbol{n}}} phases

NASA Astrophysics Data System (ADS)

Yin, Ke-Di; Zhang, Xi-Tong; Huang, Qing; Xue, Jian-Ming

2017-06-01

Ternary {M}n+1{{AX}}n phases with layered hexagonal structures, as candidate materials used for next-generation nuclear reactors, have shown great potential in tolerating radiation damage due to their unique combination of ceramic and metallic properties. However, {M}n+1{{AX}}n materials behave differently in amorphization when exposed to energetic neutron and ion irradiations in experiment. We first analyze the irradiation tolerances of different {M}n+1{{AX}}n (MAX) phases in terms of electronic structure, including the density of states (DOS) and charge density map. Then a new method based on the Bader analysis with the first-principle calculation is used to estimate the stabilities of MAX phases under irradiation. Our calculations show that the substitution of Cr/V/Ta/Nb by Ti and Si/Ge/Ga by Al can increase the ionicities of the bonds, thus strengthening the radiation tolerance. It is also shown that there is no obvious difference in radiation tolerance between {M}n+1A{{{C}}}n and {M}n+1A{{{N}}}n due to the similar charge transfer values of C and N atoms. In addition, the improved radiation tolerance from Ti3AlC2 to Ti2AlC (Ti3AlC2 and Ti2AlC have the same chemical elements), can be understood in terms of the increased Al/TiC layer ratio. Criteria based on the quantified charge transfer can be further used to explore other {M}n+1{{AX}}n phases with respect to their radiation tolerance, playing a critical role in choosing appropriate MAX phases before they are subjected to irradiation in experimental test for future nuclear reactors. Project supported by the National Natural Science Foundation of China (Grant Nos. 91226202 and 91426304).

The desert moss Pterygoneurum lamellatum (Pottiaceae) exhibits an inducible ecological strategy of desiccation tolerance: effects of rate of drying on shoot damage and regeneration

USDA-ARS?s Scientific Manuscript database

Premise of the study: Bryophytes are regarded as a clade incorporating constitutive desiccation tolerance, especially terrestrial species. Here we test the hypothesis that the opposing ecological strategy of desiccation tolerance, inducibility, is present in a desert moss, and addressed by varying r...

Freezing tolerance of winter wheat as influenced by extended growth at low temperature and exposure to freeze-thaw cycles

USDA-ARS?s Scientific Manuscript database

As the seasons progress, autumn-planted winter wheat plants (Triticum aestivum L.) first gain, then progressively lose freezing tolerance. Exposing the plants to freeze-thaw cycles of -3/3°C results in increased ability to tolerate subsequent freezing to potentially damaging temperatures. This stu...

Damage-tolerance strategies for nacre tablets.

PubMed

Wang, Shengnan; Zhu, Xinqiao; Li, Qiyang; Wang, Rizhi; Wang, Xiaoxiang

2016-05-01

Nacre, a natural armor, exhibits prominent penetration resistance against predatory attacks. Unraveling its hierarchical toughening mechanisms and damage-tolerance design strategies may provide significant inspiration for the pursuit of high-performance artificial armors. In this work, relationships between the structure and mechanical performance of nacre were investigated. The results show that other than their brick-and-mortar structure, individual nacre tablets significantly contribute to the damage localization of nacre. Affected by intracrystalline organics, the tablets exhibit a unique fracture behavior. The synergistic action of the nanoscale deformation mechanisms increases the energy dissipation efficiency of the tablets and contributes to the preservation of the structural and functional integrity of the shell. Copyright © 2016 Elsevier Inc. All rights reserved.

Damage tolerant design using collapse techniques

NASA Technical Reports Server (NTRS)

Haftka, R. T.

1982-01-01

A new approach to the design of structures for improved global damage tolerance is presented. In its undamaged condition the structure is designed subject to strength, displacement and buckling constraints. In the damaged condition the only constraint is that the structure will not collapse. The collapse load calculation is formulated as a maximization problem and solved by an interior extended penalty function. The design for minimum weight subject to constraints on the undamaged structure and a specified level of the collapse load is a minimization problem which is also solved by a penalty function formulation. Thus the overall problem is of a nested or multilevel optimization. Examples are presented to demonstrate the difference between the present and more traditional approaches.

7 CFR 51.1861 - Tolerances.

Code of Federal Regulations, 2011 CFR

2011-01-01

... damaged by shoulder bruises or by discolored or sunken scars on any parts of the tomatoes; and, (iii) Ten... percent for tomatoes which are seriously damaged by shoulder bruises or by discolored or sunken scars on... affected by decay; (ii) Ten percent for tomatoes which are seriously damaged by shoulder bruises or by...

7 CFR 51.1861 - Tolerances.

Code of Federal Regulations, 2012 CFR

2012-01-01

... damaged by shoulder bruises or by discolored or sunken scars on any parts of the tomatoes; and, (iii) Ten... percent for tomatoes which are seriously damaged by shoulder bruises or by discolored or sunken scars on... affected by decay; (ii) Ten percent for tomatoes which are seriously damaged by shoulder bruises or by...

Low Velocity Impact Damage to Carbon/Epoxy Laminates

NASA Technical Reports Server (NTRS)

Nettles, Alan T.

2011-01-01

Impact damage tends to be more detrimental to a laminate's compression strength as compared to tensile strength. Proper use of Non Destructive Evaluation (NDE) Techniques can remove conservatism (weight) from many structures. Test largest components economically feasible as coupons. If damage tolerance is a driver, then consider different resin systems. Do not use a single knockdown factor to account for damage.

Damage Tolerance of Pre-Stressed Composite Panels Under Impact Loads

NASA Astrophysics Data System (ADS)

Johnson, Alastair F.; Toso-Pentecôte, Nathalie; Schueler, Dominik

2014-02-01

An experimental test campaign studied the structural integrity of carbon fibre/epoxy panels preloaded in tension or compression then subjected to gas gun impact tests causing significant damage. The test programme used representative composite aircraft fuselage panels composed of aerospace carbon fibre toughened epoxy prepreg laminates. Preload levels in tension were representative of design limit loads for fuselage panels of this size, and maximum compression preloads were in the post-buckle region. Two main impact scenarios were considered: notch damage from a 12 mm steel cube projectile, at velocities in the range 93-136 m/s; blunt impact damage from 25 mm diameter glass balls, at velocities 64-86 m/s. The combined influence of preload and impact damage on panel residual strengths was measured and results analysed in the context of damage tolerance requirements for composite aircraft panels. The tests showed structural integrity well above design limit loads for composite panels preloaded in tension and compression with visible notch impact damage from hard body impact tests. However, blunt impact tests on buckled compression loaded panels caused large delamination damage regions which lowered plate bending stiffness and reduced significantly compression strengths in buckling.

Hypoxic pretreatment protects against neuronal damage of the rat hippocampus induced by severe hypoxia.

PubMed

Gorgias, N; Maidatsi, P; Tsolaki, M; Alvanou, A; Kiriazis, G; Kaidoglou, K; Giala, M

1996-04-01

The present study investigates whether under conditions of successive hypoxic exposures pretreatment with mild (15% O(2)) or moderate (10% O(2)) hypoxia, protects hippocampal neurones against damage induced by severe (3% O(2)) hypoxia. The ultrastructural findings were also correlated with regional superoxide dismutase (SOD) activity changes. In unpretreated rats severe hypoxia induced ultrastructural changes consistent with the aspects of delayed neuronal death (DND). However, in preexposed animals hippocampal damage was attenuated in an inversely proportional way with the severity of the hypoxic pretreatment. The ultrastructural hypoxic tolerance findings were also closely related to increased regional SOD activity levels. Thus the activation of the endogenous antioxidant defense by hypoxic preconditioning, protects against hippocampal damage induced by severe hypoxia. The eventual contribution of increased endogenous adenosine and/or reduced excitotoxicity to induce hypoxic tolerance is discussed.

Limiting damage during infection: lessons from infection tolerance for novel therapeutics.

PubMed

Vale, Pedro F; Fenton, Andy; Brown, Sam P

2014-01-01

The distinction between pathogen elimination and damage limitation during infection is beginning to change perspectives on infectious disease control, and has recently led to the development of novel therapies that focus on reducing the illness caused by pathogens (‘‘damage limitation’’)rather than reducing pathogen burdens directly (‘‘pathogen elimination’’). While beneficial at the individual host level, the population consequences of these interventions remain unclear. To address this issue,we present a simple conceptual framework for damage limitation during infection that distinguishes between therapies that are either host-centric (pro-tolerance) or pathogen-centric (anti-virulence). We then draw on recent developments from the evolutionary ecology of disease tolerance to highlight some potential epidemiological and evolutionary responses of pathogens to medical interventions that target the symptoms of infection. Just as pathogens are known to evolve in response to antimicrobial and vaccination therapies, we caution that claims of ‘‘evolution-proof’’ anti-virulence interventions may be premature, and further, that in infections where virulence and transmission are linked, reducing illness without reducing pathogen burden could have non-trivial epidemiological and evolutionary consequences that require careful examination.

Adjoint Techniques for Topology Optimization of Structures Under Damage Conditions

NASA Technical Reports Server (NTRS)

Akgun, Mehmet A.; Haftka, Raphael T.

2000-01-01

The objective of this cooperative agreement was to seek computationally efficient ways to optimize aerospace structures subject to damage tolerance criteria. Optimization was to involve sizing as well as topology optimization. The work was done in collaboration with Steve Scotti, Chauncey Wu and Joanne Walsh at the NASA Langley Research Center. Computation of constraint sensitivity is normally the most time-consuming step of an optimization procedure. The cooperative work first focused on this issue and implemented the adjoint method of sensitivity computation (Haftka and Gurdal, 1992) in an optimization code (runstream) written in Engineering Analysis Language (EAL). The method was implemented both for bar and plate elements including buckling sensitivity for the latter. Lumping of constraints was investigated as a means to reduce the computational cost. Adjoint sensitivity computation was developed and implemented for lumped stress and buckling constraints. Cost of the direct method and the adjoint method was compared for various structures with and without lumping. The results were reported in two papers (Akgun et al., 1998a and 1999). It is desirable to optimize topology of an aerospace structure subject to a large number of damage scenarios so that a damage tolerant structure is obtained. Including damage scenarios in the design procedure is critical in order to avoid large mass penalties at later stages (Haftka et al., 1983). A common method for topology optimization is that of compliance minimization (Bendsoe, 1995) which has not been used for damage tolerant design. In the present work, topology optimization is treated as a conventional problem aiming to minimize the weight subject to stress constraints. Multiple damage configurations (scenarios) are considered. Each configuration has its own structural stiffness matrix and, normally, requires factoring of the matrix and solution of the system of equations. Damage that is expected to be tolerated is local and represents a small change in the stiffness matrix compared to the baseline (undamaged) structure. The exact solution to a slightly modified set of equations can be obtained from the baseline solution economically without actually solving the modified system.. Shennan-Morrison-Woodbury (SMW) formulas are matrix update formulas that allow this (Akgun et al., 1998b). SMW formulas were therefore used here to compute adjoint displacements for sensitivity computation and structural displacements in damaged configurations.

Genome-scale analyses of butanol tolerance in Saccharomyces cerevisiae reveal an essential role of protein degradation

PubMed Central

2013-01-01

Background n-Butanol and isobutanol produced from biomass-derived sugars are promising renewable transport fuels and solvents. Saccharomyces cerevisiae has been engineered for butanol production, but its high butanol sensitivity poses an upper limit to product titers that can be reached by further pathway engineering. A better understanding of the molecular basis of butanol stress and tolerance of S. cerevisiae is important for achieving improved tolerance. Results By combining a screening of the haploid S. cerevisiae knock-out library, gene overexpression, and genome analysis of evolutionary engineered n-butanol-tolerant strains, we established that protein degradation plays an essential role in tolerance. Strains deleted in genes involved in the ubiquitin-proteasome system and in vacuolar degradation of damaged proteins showed hypersensitivity to n-butanol. Overexpression of YLR224W, encoding the subunit responsible for the recognition of damaged proteins of an ubiquitin ligase complex, resulted in a strain with a higher n-butanol tolerance. Two independently evolved n-butanol-tolerant strains carried different mutations in both RPN4 and RTG1, which encode transcription factors involved in the expression of proteasome and peroxisomal genes, respectively. Introduction of these mutated alleles in the reference strain increased butanol tolerance, confirming their relevance in the higher tolerance phenotype. The evolved strains, in addition to n-butanol, were also more tolerant to 2-butanol, isobutanol and 1-propanol, indicating a common molecular basis for sensitivity and tolerance to C3 and C4 alcohols. Conclusions This study shows that maintenance of protein integrity plays an essential role in butanol tolerance and demonstrates new promising targets to engineer S. cerevisiae for improved tolerance. PMID:23552365

Tension-Compression Fatigue of a Nextel™720/alumina Composite at 1200 °C in Air and in Steam

NASA Astrophysics Data System (ADS)

Lanser, R. L.; Ruggles-Wrenn, M. B.

2016-08-01

Tension-compression fatigue behavior of an oxide-oxide ceramic-matrix composite was investigated at 1200 °C in air and in steam. The composite is comprised of an alumina matrix reinforced with Nextel™720 alumina-mullite fibers woven in an eight harness satin weave (8HSW). The composite has no interface between the fiber and matrix, and relies on the porous matrix for flaw tolerance. Tension-compression fatigue behavior was studied for cyclical stresses ranging from 60 to 120 MPa at a frequency of 1.0 Hz. The R ratio (minimum stress to maximum stress) was -1.0. Fatigue run-out was defined as 105 cycles and was achieved at 80 MPa in air and at 70 MPa in steam. Steam reduced cyclic lives by an order of magnitude. Specimens that achieved fatigue run-out were subjected to tensile tests to failure to characterize the retained tensile properties. Specimens subjected to prior cyclic loading in air retained 100 % of their tensile strength. The steam environment severely degraded tensile properties. Tension-compression cyclic loading was considerably more damaging than tension-tension cyclic loading. Composite microstructure, as well as damage and failure mechanisms were investigated.

Lipophilic components of the brown seaweed, Ascophyllum nodosum, enhance freezing tolerance in Arabidopsis thaliana.

PubMed

Rayirath, Prasanth; Benkel, Bernhard; Mark Hodges, D; Allan-Wojtas, Paula; Mackinnon, Shawna; Critchley, Alan T; Prithiviraj, Balakrishnan

2009-06-01

Extracts of the brown seaweed Ascophyllum nodosum enhance plant tolerance against environmental stresses such as drought, salinity, and frost. However, the molecular mechanisms underlying this improved stress tolerance and the nature of the bioactive compounds present in the seaweed extracts that elicits stress tolerance remain largely unknown. We investigated the effect of A. nodosum extracts and its organic sub-fractions on freezing tolerance of Arabidopsis thaliana. Ascophyllum nodosum extracts and its lipophilic fraction significantly increased tolerance to freezing temperatures in in vitro and in vivo assays. Untreated plants exhibited severe chlorosis, tissue damage, and failed to recover from freezing treatments while the extract-treated plants recovered from freezing temperature of -7.5 degrees C in in vitro and -5.5 degrees C in in vivo assays. Electrolyte leakage measurements revealed that the LT(50) value was lowered by 3 degrees C while cell viability staining demonstrated a 30-40% reduction in area of damaged tissue in extract treated plants as compared to water controls. Moreover, histological observations of leaf sections revealed that extracts have a significant effect on maintaining membrane integrity during freezing stress. Treated plants exhibited 70% less chlorophyll damage during freezing recovery as compared to the controls, and this correlated with reduced expression of the chlorphyllase genes AtCHL1 and AtCHL2. Further, the A. nodosum extract treatment modulated the expression of the cold response genes, COR15A, RD29A, and CBF3, resulting in enhanced tolerance to freezing temperatures. More than 2.6-fold increase in expression of RD29A, 1.8-fold increase of CBF3 and two-fold increase in the transcript level of COR15A was observed in plants treated with lipophilic fraction of A. nodosum at -2 degrees C. Taken together, the results suggest that chemical components in A. nodosum extracts protect membrane integrity and affect the expression of stress response genes leading to freezing stress tolerance in A. thaliana.

Ocular tolerance of preservatives on the murine cornea.

PubMed

Furrer, P; Mayer, J M; Plazonnet, B; Gurny, R

1999-03-01

We investigated the effects of instilling 13 commonly used preservatives on the murine cornea in vivo. Due to the instillation of preservatives, micro-lesions are formed on the cornea and can be selectively marked by fluorescein. The sum of the resulting fluorescent areas was measured using an episcopic microscope coupled to an image processing system. All the tested preservatives proved to be well-tolerated by the eye at commonly used concentrations. However, in some cases, increased concentrations of preservatives or combinations resulted in significant increase of the amount of corneal damage. With increasing the concentration, corneal lesion increased the most in the case of cetylpyridinium. While a combination of chlorobutanol 0.5% and phenylethylalcohol 0.5% did not result in an increase in corneal damage (when compared to the use of each separately), the associations of thiomersal 0.02% and phenylethylalcohol 0.4% on one hand and of edetate disodium (EDTA) 0.1% and benzalkonium 0.01% on the other, resulted in significant increases in the amount of corneal damage. However, in none of the tested combinations, the increase in the observed damage exceed the limit of ocular intolerance we had defined beforehand: thus, they were all deemed relatively well-tolerated. In the last part of the study, we investigated the effects of combining several preservatives, at usual concentrations, with an anesthetic solution of oxybuprocaine and found no notable increase in ocular damage.

Recent Advances in Thermoplastic Puncture-Healing Polymers

NASA Technical Reports Server (NTRS)

Gordon, K. L.; Working, D. C.; Wise, K. E.; Bogert, P. B.; Britton, S. M.; Topping, C.C.; Smith, J. Y.; Siochi, E. J.

2009-01-01

Self-healing materials provide a route for enhanced damage tolerance in materials for aerospace applications. In particular, puncture-healing upon impact has the potential to mitigate significant damage caused by high velocity micrometeoroid impacts. This type of material also has the potential to improve damage tolerance in load bearing structures to enhance vehicle health and aircraft durability. The materials being studied are those capable of instantaneous puncture healing, providing a mechanism for mechanical property retention in lightweight structures. These systems have demonstrated healing capability following penetration of fast moving projectiles -- velocities that range from 9 mm bullets shot from a gun (approx.330 m/sec) to close to micrometeoroid debris velocities of 4800 m/sec. In this presentation, we report on a suite of polymeric materials possessing this characteristic. Figure 1 illustrates the puncture healing concept. Puncture healing in these materials is dependent upon how the combination of a polymer's viscoelastic properties responds to the energy input resulting from the puncture event. Projectile penetration increases the temperature in the vicinity of the impact. Self-healing behavior occurs following puncture, whereby energy must be transferred to the material during impact both elastically and inelastically, thus establishing two requirements for puncture healing to occur: a.) The need for the puncture event to produce a local melt state in the polymer material and b.) The molten material has to have sufficient melt elasticity to snap back and close the hole. 1,2 Previous ballistic testing studies revealed that Surlyn materials warmed up to a temperature approx.98 C during projectile puncture (3 C higher than it s melting temperature). 1,2 The temperature increase produces a localized flow state and the melt elasticity to snap back thus sealing the hole. Table 1 lists the commercially polymers studied here, together with their physical properties. The polymers were selected based on chemical structure, tensile strengths, tensile moduli, glass transition temperature, melting temperatures, and impact strength. The thermal properties of the polymers were characterized by Differential Scanning Calorimetry (DSC) and Dynamic Mechanical Analysis (DMA). Mechanical properties were assessed by a Sintech 2W instron according to ASTM D1708 or D638 at crosshead speeds of 5.08 cm/min. 7.6 cm x 7.6 cm panels of the different materials were prepared and ballistic testing was performed at various temperatures. The panels were shot with a .223 caliber semiautomatic rifle from a distance of 23 meters at various temperatures. Chronographs were used to measure initial and final bullet velocity. Temperatures at the site of impact were measured using a FLIR ThermaCAM S60 thermal camera. A Vision Research model Phantom 9 high speed video camera was used to capture high speed video footage of ballistics testing.

Radiation Tolerance of 65nm CMOS Transistors

DOE PAGES

Krohn, M.; Bentele, B.; Christian, D. C.; ...

2015-12-11

We report on the effects of ionizing radiation on 65 nm CMOS transistors held at approximately -20°C during irradiation. The pattern of damage observed after a total dose of 1 Grad is similar to damage reported in room temperature exposures, but we observe less damage than was observed at room temperature.

Damage tolerance of a composite sandwich with interleaved foam core

NASA Astrophysics Data System (ADS)

Ishai, Ori; Hiel, Clement

A composite sandwich panel consisting of carbon fiber-reinforced plastic (CFRP) skins and a syntactic foam core was selected as an appropriate structural concept for the design of wind tunnel compressor blades. Interleaving of the core with tough interlayers was done to prevent core cracking and to improve damage tolerance of the sandwich. Simply supported sandwich beam specimens were subjected to low-velocity drop-weight impacts as well as high velocity ballistic impacts. The performance of the interleaved core sandwich panels was characterized by localized skin damage and minor cracking of the core. Residual compressive strength (RCS) of the skin, which was derived from flexural test, shows the expected trend of decreasing with increasing size of the damage, impact energy, and velocity. In the case of skin damage, RCS values of around 50 percent of the virgin interleaved reference were obtained at the upper impact energy range. Based on the similarity between low-velocity and ballistic-impact effects, it was concluded that impact energy is the main variable controlling damage and residual strength, where as velocity plays a minor role.

Damage tolerance of a composite sandwich with interleaved foam core

NASA Technical Reports Server (NTRS)

Ishai, Ori; Hiel, Clement

1992-01-01

A composite sandwich panel consisting of carbon fiber-reinforced plastic (CFRP) skins and a syntactic foam core was selected as an appropriate structural concept for the design of wind tunnel compressor blades. Interleaving of the core with tough interlayers was done to prevent core cracking and to improve damage tolerance of the sandwich. Simply supported sandwich beam specimens were subjected to low-velocity drop-weight impacts as well as high velocity ballistic impacts. The performance of the interleaved core sandwich panels was characterized by localized skin damage and minor cracking of the core. Residual compressive strength (RCS) of the skin, which was derived from flexural test, shows the expected trend of decreasing with increasing size of the damage, impact energy, and velocity. In the case of skin damage, RCS values of around 50 percent of the virgin interleaved reference were obtained at the upper impact energy range. Based on the similarity between low-velocity and ballistic-impact effects, it was concluded that impact energy is the main variable controlling damage and residual strength, where as velocity plays a minor role.

Damage-Tolerance Characteristics of Composite Fuselage Sandwich Structures with Thick Facesheets

NASA Technical Reports Server (NTRS)

McGowan, David M.; Ambur, Damodar R.

1997-01-01

Damage tolerance characteristics and results from experimental and analytical studies of a composite fuselage keel sandwich structure subjected to low-speed impact damage and discrete-source damage are presented. The test specimens are constructed from graphite-epoxy skins borided to a honeycomb core, and they are representative of a highly loaded fuselage keel structure. Results of compression-after-impact (CAI) and notch-length sensitivity studies of 5-in.-wide by 10-in.long specimens are presented. A correlation between low-speed-impact dent depth, the associated damage area, and residual strength for different impact-energy levels is described; and a comparison of the strength for undamaged and damaged specimens with different notch-length-to-specimen-width ratios is presented. Surface strains in the facesheets of the undamaged specimens as well as surface strains that illustrate the load redistribution around the notch sites in the notched specimens are presented and compared with results from finite element analyses. Reductions in strength of as much as 53.1 percent for the impacted specimens and 64.7 percent for the notched specimens are observed.

Strengthening of ferrous binder jet 3D printed components through bronze infiltration

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cordero, Zachary C.; Siddel, Derek H.; Peter, William H.

Fully-dense, net shape objects have been fabricated from a rapidly-solidified ferrous powder using binder-jet 3D printing and molten bronze infiltration. X-ray diffraction, scanning electron microscopy, and differential thermal analysis were used to characterize the structural evolution of the powder feedstock during an infiltration heating cycle. Microindentation and bend tests were performed on the infiltrated material to evaluate its mechanical properties. The infiltrated material had an 11 GPa hardness and moderate damage tolerance. It was found that infiltration improved both the ductility and strength of the sintered preforms by eliminating the stress concentration at the interparticle necks.

Strengthening of ferrous binder jet 3D printed components through bronze infiltration

DOE PAGES

Cordero, Zachary C.; Siddel, Derek H.; Peter, William H.; ...

2017-04-08

Fully-dense, net shape objects have been fabricated from a rapidly-solidified ferrous powder using binder-jet 3D printing and molten bronze infiltration. X-ray diffraction, scanning electron microscopy, and differential thermal analysis were used to characterize the structural evolution of the powder feedstock during an infiltration heating cycle. Microindentation and bend tests were performed on the infiltrated material to evaluate its mechanical properties. The infiltrated material had an 11 GPa hardness and moderate damage tolerance. It was found that infiltration improved both the ductility and strength of the sintered preforms by eliminating the stress concentration at the interparticle necks.

40 CFR 180.1180 - Kaolin; exemption from the requirement of a tolerance.

Code of Federal Regulations, 2014 CFR

2014-07-01

..., fungus, and bacterial damage to plants. This temporary exemption from the requirement of a tolerance will... control of insects, fungi, and bacteria (food/feed use). [62 FR 19685, Apr. 23, 1997, as amended at 63 FR...

40 CFR 180.1180 - Kaolin; exemption from the requirement of a tolerance.

Code of Federal Regulations, 2012 CFR

2012-07-01

..., fungus, and bacterial damage to plants. This temporary exemption from the requirement of a tolerance will... control of insects, fungi, and bacteria (food/feed use). [62 FR 19685, Apr. 23, 1997, as amended at 63 FR...

40 CFR 180.1180 - Kaolin; exemption from the requirement of a tolerance.

Code of Federal Regulations, 2013 CFR

2013-07-01

..., fungus, and bacterial damage to plants. This temporary exemption from the requirement of a tolerance will... control of insects, fungi, and bacteria (food/feed use). [62 FR 19685, Apr. 23, 1997, as amended at 63 FR...

Seasonal variation in hybrid poplar tolerance to glyphosate.

Treesearch

Daniel Netzer; Edward Hansen

1992-01-01

Reports that glyphosate applied during April or May in hybrid poplar plantations usually results in tree growth increases and that later summer applications often result in tree damage, growth loss, or mortality. Introduces the concept of "physiological" and "morphological" herbicide tolerance.

40 CFR 180.1180 - Kaolin; exemption from the requirement of a tolerance.

Code of Federal Regulations, 2010 CFR

2010-07-01

..., fungus, and bacterial damage to plants. This temporary exemption from the requirement of a tolerance will... control of insects, fungi, and bacteria (food/feed use). [62 FR 19685, Apr. 23, 1997, as amended at 63 FR...

40 CFR 180.1180 - Kaolin; exemption from the requirement of a tolerance.

Code of Federal Regulations, 2011 CFR

2011-07-01

..., fungus, and bacterial damage to plants. This temporary exemption from the requirement of a tolerance will... control of insects, fungi, and bacteria (food/feed use). [62 FR 19685, Apr. 23, 1997, as amended at 63 FR...

Effects of through-the-thickness stitching on impact and interlaminar fracture properties of textile graphite/epoxy laminates

NASA Technical Reports Server (NTRS)

Sharma, Suresh K.; Sankar, Bhavani V.

1995-01-01

This study investigated the effects of through-the-thickness stitching on impact damage resistance, impact damage tolerance, and mode I and mode II fracture toughness of textile graphite/epoxy laminates. Uniweave resin-transfer-molded 48 ply graphite/epoxy (AS4/3501-6) laminates were stitched with Kevlar and glass yarns of different linear densities and stitch spacings. Delaminations were implanted during processing to simulate impact damage. Sublaminate buckling tests were performed to determine the effects of stitching on the compressive strength. The results showed outstanding improvements of up to 400 percent in the compression strength over the unstitched laminates. In impact and static indentation tests the onset of damage occurred at the same level, but the extent of damage was less in stitched laminates. Mode I fracture toughness of 24 ply Uniweave unidirectional (AS4/3501-6) stitched laminates was measured by conducting double-cantilever-beam tests. The critical strain energy release rate (G(sub Ic)) was found to be up to 30 times higher than the unstitched laminates. Mode II fracture toughness of the Uniweave laminates was measured by performing end-notched-flexure tests. Two new methods to compute the apparent G(sub IIc) are presented. The apparent G(sub IIc) was found to be at least 5-15 times higher for the stitched laminates.

USAF Damage Tolerant Design Handbook: Guidelines for the analysis and Design of Damage Tolerant Aircraft Structures. Revision A

DTIC Science & Technology

1979-03-01

Fatigue Crack Growth (Schr~matic) 5.12 Sustained Load Crack Growth Rate Data for 7075-f651,7079- T651, and 2024 - T351 Aluminum Plate (Ref...Block Programming and Block Size on Crack Growth Life (All histories Have Same Cycle Content) Alloy : 2024 -T3 Aluminum (Ref. 38) 5.21 Yield Zone Due to...4340 Steel in Humid Air," ASM Trans 58, 46-53 (1965). 20. Meyn, D.A., "Frequency and Amplitude Effects on Corrosion Fatigue Cracks in a Titanium Alloy

USAF Damage Tolerant Design Handbook: Guidelines for the Analysis and Design of Damage Tolerant Aircraft Structures. Revision B

DTIC Science & Technology

1984-05-01

Effect of Block Programming and Block Size on Crack Growth Life (All Histories Have Same Cycle Content) Alloy : 2024 -T3 Aluminum (Reference 27...1 I 6 o T 1 >0 100 Crack Growth Life for l-in. Crack Growth 1000 Figure 5.1.10, Effect of Humidity on Fatigue Crack Propagation...Growth Life (All Histories Have Same Cycle Content) Alloy : 2024 -T3 Aluminum (Reference 27). 5.2.6 Yield Zone Due to Overload (r T), Current Crack

International SAMPE Symposium and Exhibition, 35th, Anaheim, CA, Apr. 2-5, 1990, Proceedings. Books 1 2

DOE Office of Scientific and Technical Information (OSTI.GOV)

Janicki, G.; Bailey, V.; Schjelderup, H.

The present conference discusses topics in the fields of ultralightweight structures, producibility of thermoplastic composites, innovation in sandwich structures, composite failure processes, toughened materials, metal-matrix composites, advanced materials for future naval systems, thermoplastic polymers, automated composites manufacturers, advanced adhesives, emerging processes for aerospace component fabrication, and modified resin systems. Also discussed are matrix behavior for damage tolerance, composite materials repair, testing for damage tolerance, composite strength analyses, materials workplace health and safety, cost-conscious composites, bismaleimide systems, and issues facing advanced composite materials suppliers.

A preliminary damage tolerance methodology for composite structures

NASA Technical Reports Server (NTRS)

Wilkins, D. J.

1983-01-01

The certification experience for the primary, safety-of-flight composite structure applications on the F-16 is discussed. The rationale for the selection of delamination as the major issue for damage tolerance is discussed, as well as the modeling approach selected. The development of the necessary coupon-level data base is briefly summarized. The major emphasis is on the description of a full-scale fatigue test where delamination growth was obtained to demonstrate the validity of the selected approach. A summary is used to review the generic features of the methodology.

Requirements of Inconel 718 alloy for aeronautical applications

NASA Astrophysics Data System (ADS)

Ghiban, Brandusa; Elefterie, Cornelia Florina; Guragata, Constantin; Bran, Dragos

2018-02-01

The main requirements imposed by aviation components made from super alloys based on Nickel are presented in present paper. A significant portion of fasteners, locking lugs, blade retainers and inserts are manufactured from Inconel 718 alloy. The thesis describes environmental factors (corrosion), conditions of external aggression (salt air, intense heat, heavy industrial pollution, high condensation, high pressure), mechanical characteristics (tensile strength, creep, density, yield strength, fracture toughness, fatigue resistance) and loadings (tensions, compression loads) that must be satisfied simultaneously by Ni-based super alloy, compared to other classes of aviation alloys (as egg. Titanium alloys, Aluminum alloys). For this alloy the requirements are strength, durability, damage tolerance, fail safety and so on. The corrosion can be an issue, but the fatigue under high-magnitude cyclic tensile loading it what limits the lifetime of the airframe. The excellent malleability and weldability characteristics of the 718 system make the material physical properties tolerant of manufacturing processes. These characteristics additionally continue to provide new opportunities for advanced manufacturing methods.

Aeronautical requirements for Inconel 718 alloy

NASA Astrophysics Data System (ADS)

Elefterie, C. F.; Guragata, C.; Bran, D.; Ghiban, B.

2017-06-01

The project goal is to present the requirements imposed by aviation components made from super alloys based on Nickel. A significant portion of fasteners, locking lugs, blade retainers and inserts are manufactured from Alloy 718. The thesis describes environmental factors (corrosion), conditions of external aggression (salt air, intense heat, heavy industrial pollution, high condensation, high pressure), mechanical characteristics (tensile strength, yield strength and fatigue resistance) and loadings (tensions, compression loads) that must be satisfied simultaneously by Ni-based super alloy, compared to other classes of aviation alloys (as egg. Titanium alloys, Aluminum alloys). For this alloy the requirements are strength durability, damage tolerance, fail safety and so on. The corrosion can be an issue, but the fatigue under high-magnitude cyclic tensile loading it’s what limits the lifetime of the airframe. Also, the excellent malleability and weldability characteristics of the 718 system make the material physical properties tolerant of manufacturing processes. These characteristics additionally continue to provide new opportunities for advanced manufacturing methods.

Modelling biofilm-induced formation damage and biocide treatment in subsurface geosystems

PubMed Central

Ezeuko, C C; Sen, A; Gates, I D

2013-01-01

Biofilm growth in subsurface porous media, and its treatment with biocides (antimicrobial agents), involves a complex interaction of biogeochemical processes which provide non-trivial mathematical modelling challenges. Although there are literature reports of mathematical models to evaluate biofilm tolerance to biocides, none of these models have investigated biocide treatment of biofilms growing in interconnected porous media with flow. In this paper, we present a numerical investigation using a pore network model of biofilm growth, formation damage and biocide treatment. The model includes three phases (aqueous, adsorbed biofilm, and solid matrix), a single growth-limiting nutrient and a single biocide dissolved in the water. Biofilm is assumed to contain a single species of microbe, in which each cell can be a viable persister, a viable non-persister, or non-viable (dead). Persisters describe small subpopulation of cells which are tolerant to biocide treatment. Biofilm tolerance to biocide treatment is regulated by persister cells and includes ‘innate’ and ‘biocide-induced’ factors. Simulations demonstrate that biofilm tolerance to biocides can increase with biofilm maturity, and that biocide treatment alone does not reverse biofilm-induced formation damage. Also, a successful application of biological permeability conformance treatment involving geologic layers with flow communication is more complicated than simply engineering the attachment of biofilm-forming cells at desired sites. PMID:23164434

Temperature-stress resistance and tolerance along a latitudinal cline in North American Arabidopsis lyrata.

PubMed

Wos, Guillaume; Willi, Yvonne

2015-01-01

The study of latitudinal gradients can yield important insights into adaptation to temperature stress. Two strategies are available: resistance by limiting damage, or tolerance by reducing the fitness consequences of damage. Here we studied latitudinal variation in resistance and tolerance to frost and heat and tested the prediction of a trade-off between the two strategies and their costliness. We raised plants of replicate maternal seed families from eight populations of North American Arabidopsis lyrata collected along a latitudinal gradient in climate chambers and exposed them repeatedly to either frost or heat stress, while a set of control plants grew under standard conditions. When control plants reached maximum rosette size, leaf samples were exposed to frost and heat stress, and electrolyte leakage (PEL) was measured and treated as an estimate of resistance. Difference in maximum rosette size between stressed and control plants was used as an estimate of tolerance. Northern populations were more frost resistant, and less heat resistant and less heat tolerant, but-unexpectedly-they were also less frost tolerant. Negative genetic correlations between resistance and tolerance to the same and different thermal stress were generally not significant, indicating only weak trade-offs. However, tolerance to frost was consistently accompanied by small size under control conditions, which may explain the non-adaptive latitudinal pattern for frost tolerance. Our results suggest that adaptation to frost and heat is not constrained by trade-offs between them. But the cost of frost tolerance in terms of plant size reduction may be important for the limits of species distributions and climate niches.

The roles of tolerance in the evolution, maintenance and breakdown of mutualism

NASA Astrophysics Data System (ADS)

Edwards, David P.

2009-10-01

Tolerance strategies are cost-reduction mechanisms that enable organisms to recover some of the fitness lost to damage, but impose limited or no cost on antagonists. They are frequently invoked in studies of plant-herbivore and of host-parasite interactions, but the possible roles of tolerance in mutualism (interspecific cooperation) have yet to be thoroughly examined. This review identifies candidate roles for tolerance in the evolution, maintenance and breakdown of mutualism. Firstly, by reducing the cost of damage, tolerance provides a key pathway by which pre-mutualistic hosts can reduce the cost of association with their parasites, promoting cooperation. This holds for the evolution of ‘evolved dependency’ type mutualism, where a host requires an antagonist that does not direct any reward to their partner for some resource, and of ‘outright mutualism’, where participants directly trade benefits. Secondly, in outright mutualism, tolerance might maintain cooperation by reducing the cost of a persisting negative trait in a symbiotic partner. Finally, the evolution of tolerance might also provide a pathway out of mutualism because the host could evolve a cheaper alternative to continued cooperation with its mutualistic partner, permitting autonomy. A key consequence of tolerance is that it contrasts with partner choice mechanisms that impose large costs on cheats, and I highlight understanding any trade-off between tolerance and partner choice as an important research topic in the evolution of cooperation. I conclude by identifying tolerance as part of a more general phenomenon of co-adaptation in mutualism and parasitism that drives the evolution of the cost/benefit ratio from the interaction.

Biology and control of Varroa destructor.

PubMed

Rosenkranz, Peter; Aumeier, Pia; Ziegelmann, Bettina

2010-01-01

The ectoparasitic honey bee mite Varroa destructor was originally confined to the Eastern honey bee Apis cerana. After a shift to the new host Apis mellifera during the first half of the last century, the parasite dispersed world wide and is currently considered the major threat for apiculture. The damage caused by Varroosis is thought to be a crucial driver for the periodical colony losses in Europe and the USA and regular Varroa treatments are essential in these countries. Therefore, Varroa research not only deals with a fascinating host-parasite relationship but also has a responsibility to find sustainable solutions for the beekeeping. This review provides a survey of the current knowledge in the main fields of Varroa research including the biology of the mite, damage to the host, host tolerance, tolerance breeding and Varroa treatment. We first present a general view on the functional morphology and on the biology of the Varroa mite with special emphasis on host-parasite interactions during reproduction of the female mite. The pathology section describes host damage at the individual and colony level including the problem of transmission of secondary infections by the mite. Knowledge of both the biology and the pathology of Varroa mites is essential for understanding possible tolerance mechanisms in the honey bee host. We comment on the few examples of natural tolerance in A. mellifera and evaluate recent approaches to the selection of Varroa tolerant honey bees. Finally, an extensive listing and critical evaluation of chemical and biological methods of Varroa treatments is given. This compilation of present-day knowledge on Varroa honey bee interactions emphasizes that we are still far from a solution for Varroa infestation and that, therefore, further research on mite biology, tolerance breeding, and Varroa treatment is urgently needed. Copyright 2009 Elsevier Inc. All rights reserved.

Effects of Oxygen Availability on Acetic Acid Tolerance and Intracellular pH in Dekkera bruxellensis.

PubMed

Capusoni, Claudia; Arioli, Stefania; Zambelli, Paolo; Moktaduzzaman, M; Mora, Diego; Compagno, Concetta

2016-08-01

The yeast Dekkera bruxellensis, associated with wine and beer production, has recently received attention, because its high ethanol and acid tolerance enables it to compete with Saccharomyces cerevisiae in distilleries that produce fuel ethanol. We investigated how different cultivation conditions affect the acetic acid tolerance of D. bruxellensis We analyzed the ability of two strains (CBS 98 and CBS 4482) exhibiting different degrees of tolerance to grow in the presence of acetic acid under aerobic and oxygen-limited conditions. We found that the concomitant presence of acetic acid and oxygen had a negative effect on D. bruxellensis growth. In contrast, incubation under oxygen-limited conditions resulted in reproducible growth kinetics that exhibited a shorter adaptive phase and higher growth rates than those with cultivation under aerobic conditions. This positive effect was more pronounced in CBS 98, the more-sensitive strain. Cultivation of CBS 98 cells under oxygen-limited conditions improved their ability to restore their intracellular pH upon acetic acid exposure and to reduce the oxidative damage to intracellular macromolecules caused by the presence of acetic acid. This study reveals an important role of oxidative stress in acetic acid tolerance in D. bruxellensis, indicating that reduced oxygen availability can protect against the damage caused by the presence of acetic acid. This aspect is important for optimizing industrial processes performed in the presence of acetic acid. This study reveals an important role of oxidative stress in acetic acid tolerance in D. bruxellensis, indicating that reduced oxygen availability can have a protective role against the damage caused by the presence of acetic acid. This aspect is important for the optimization of industrial processes performed in the presence of acetic acid. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

Effects of Oxygen Availability on Acetic Acid Tolerance and Intracellular pH in Dekkera bruxellensis

PubMed Central

Capusoni, Claudia; Arioli, Stefania; Zambelli, Paolo; Moktaduzzaman, M.; Mora, Diego

2016-01-01

ABSTRACT The yeast Dekkera bruxellensis, associated with wine and beer production, has recently received attention, because its high ethanol and acid tolerance enables it to compete with Saccharomyces cerevisiae in distilleries that produce fuel ethanol. We investigated how different cultivation conditions affect the acetic acid tolerance of D. bruxellensis. We analyzed the ability of two strains (CBS 98 and CBS 4482) exhibiting different degrees of tolerance to grow in the presence of acetic acid under aerobic and oxygen-limited conditions. We found that the concomitant presence of acetic acid and oxygen had a negative effect on D. bruxellensis growth. In contrast, incubation under oxygen-limited conditions resulted in reproducible growth kinetics that exhibited a shorter adaptive phase and higher growth rates than those with cultivation under aerobic conditions. This positive effect was more pronounced in CBS 98, the more-sensitive strain. Cultivation of CBS 98 cells under oxygen-limited conditions improved their ability to restore their intracellular pH upon acetic acid exposure and to reduce the oxidative damage to intracellular macromolecules caused by the presence of acetic acid. This study reveals an important role of oxidative stress in acetic acid tolerance in D. bruxellensis, indicating that reduced oxygen availability can protect against the damage caused by the presence of acetic acid. This aspect is important for optimizing industrial processes performed in the presence of acetic acid. IMPORTANCE This study reveals an important role of oxidative stress in acetic acid tolerance in D. bruxellensis, indicating that reduced oxygen availability can have a protective role against the damage caused by the presence of acetic acid. This aspect is important for the optimization of industrial processes performed in the presence of acetic acid. PMID:27235432

Out-of-plane properties

NASA Technical Reports Server (NTRS)

Jackson, Wade C.; Portanova, Marc A.

1995-01-01

This paper summarizes three areas of research which were performed to characterize out-of-plane properties of composite materials. In the first investigation, a series of tests was run to characterize the through-the-thickness tensile strength for a variety of composites that included 2D braids, 2D and 3D weaves, and prepreg tapes. A new test method based on a curved beam was evaluated. Failures were significantly different between the 2D materials and the 3D weaves. The 2D materials delaminated between layers due to out-of-plane tensile stresses while the 3D weaves failed due to the formation of radial cracks between the surface plies caused by high circumferential stresses along the inner radius. The strength of the 2D textile composites did not increase relative to the tapes. Final failure in the 3D weaves was caused by a circumferential crack similar to the 2D materials and occurred at a lower bending moment than in other materials. The early failures in the 3D weaves were caused by radial crack formation rather than a low through-the-thickness strength. The second investigation focused on the development of a standard impact test method to measure impact damage resistance. The only impact tests that currently exist are compression after impact (CAI) tests which incorporate elements of both damage resistance and damage tolerance. A new impact test method is under development which uses a quasi-static indentation (QSI) test to directly measure damage resistance. Damage resistance is quantified in terms of the contact force to produce a unit of damage where a metric for damage may be area in C-scan, depth of residual dent , penetration, damage growth, etc. A final draft of an impact standard that uses a QSI test method will be presented to the ASTM Impact Task Group on impact. In the third investigation, the impact damage resistance behavior of a variety of textile materials was studied using the QSI test method. In this study, the force where large damage initiates was measured and the delamination size as a function of force was determined. The force to initiate large damage was significantly lower in braids and weaves. The delamination diameter - impact forace relationship was quanitfied using a damage resistance parameter, Q(*), which related delamination diameter to imapct force over a range of delamination sizes. Using this Q(*) parameter to rate the materials, the stitched uniweaves, toughened epoxy tapes, and through-the-thickness orthogonal interlock weave were the most damage resistant.

Overexpression of the DNA mismatch repair factor, PMS2, confers hypermutability and DNA damage tolerance.

PubMed

Gibson, Shannon L; Narayanan, Latha; Hegan, Denise Campisi; Buermeyer, Andrew B; Liskay, R Michael; Glazer, Peter M

2006-12-08

Inherited defects in genes associated with DNA mismatch repair (MMR) have been linked to familial colorectal cancer. Cells deficient in MMR are genetically unstable and demonstrate a tolerance phenotype in response to certain classes of DNA damage. Some sporadic human cancers also show abnormalities in MMR gene function, typically due to diminished expression of one of the MutL homologs, MLH1. Here, we report that overexpression of the MutL homolog, human PMS2, can also cause a disruption of the MMR pathway in mammalian cells, resulting in hypermutability and DNA damage tolerance. A mouse fibroblast cell line carrying a recoverable lambda phage shuttle vector for mutation detection was transfected with either a vector designed to express hPMS2 or with an empty vector control. Cells overexpressing hPMS2 were found to have elevated spontaneous mutation frequencies at the cII reporter gene locus. They also showed an increase in the level of mutations induced by the alkylating agent, methynitrosourea (MNU). Clonogenic survival assays demonstrated increased survival of the PMS2-overexpressing cells following exposure to MNU, consistent with the induction of a damage tolerance phenotype. Similar results were seen in cells expressing a mutant PMS2 gene, containing a premature stop codon at position 134 and representing a variant found in an individual with familial colon cancer. These results show that dysregulation of PMS2 gene expression can disrupt MMR function in mammalian cells and establish an additional carcinogenic mechanism by which cells can develop genetic instability and acquire resistance to cytotoxic cancer therapies.

Tolerance of Cottonwood to Damage by Cottonwood Leaf Beetle

Treesearch

F. L. Oliveria; D. T. Cooper

1977-01-01

Wide variation in tolerance to the cottonwood leaf beetle was found in fourteen hundred eastern cottonwood clones, originating from 36 young natural stands along the Mississippi River from Memphis, Tennessee, to Baton Rouge, Louisiana. Expected genetic gains were large enough to justify further research.

Overexpression of CsCaM3 Improves High Temperature Tolerance in Cucumber

PubMed Central

Yu, Bingwei; Yan, Shuangshuang; Zhou, Huoyan; Dong, Riyue; Lei, Jianjun; Chen, Changming; Cao, Bihao

2018-01-01

High temperature (HT) stress affects the growth and production of cucumbers, but genetic resources with high heat tolerance are very scarce in this crop. Calmodulin (CaM) has been confirmed to be related to the regulation of HT stress resistance in plants. CsCaM3, a CaM gene, was isolated from cucumber inbred line “02-8.” Its expression was characterized in the present study. CsCaM3 transcripts differed among the organs and tissues of cucumber plants and could be induced by HTs or abscisic acid, but not by salicylic acid. CsCaM3 transcripts exhibited subcellular localization to the cytoplasm and nuclei of cells. Overexpression of CsCaM3 in cucumber plants has the potential to improve their heat tolerance and protect against oxidative damage and photosynthesis system damage by regulating the expression of HT-responsive genes in plants, including chlorophyll catabolism-related genes under HT stress. Taken together, our results provide useful insights into stress tolerance in cucumber. PMID:29946334

Biochemical indicators of root damage in rice (Oryza sativa) genotypes under zinc deficiency stress.

PubMed

Lee, Jae-Sung; Wissuwa, Matthias; Zamora, Oscar B; Ismail, Abdelbagi M

2017-11-01

Zn deficiency is one of the major soil constraints currently limiting rice production. Although recent studies demonstrated that higher antioxidant activity in leaf tissue effectively protects against Zn deficiency stress, little is known about whether similar tolerance mechanisms operate in root tissue. In this study we explored root-specific responses of different rice genotypes to Zn deficiency. Root solute leakage and biomass reduction, antioxidant activity, and metabolic changes were measured using plants grown in Zn-deficient soil and hydroponics. Solute leakage from roots was higher in sensitive genotypes and linked to membrane damage caused by Zn deficiency-induced oxidative stress. However, total root antioxidant activity was four-fold lower than in leaves and did not differ between sensitive and tolerant genotypes. Root metabolite analysis using gas chromatography-mass spectrometry and high performance liquid chromatography indicated that Zn deficiency triggered the accumulation of glycerol-3-phosphate and acetate in sensitive genotypes, while less or no accumulation was seen in tolerant genotypes. We suggest that these metabolites may serve as biochemical indicators of root damage under Zn deficiency.

Properties of Two Carbon Composite Materials Using LTM25 Epoxy Resin

NASA Technical Reports Server (NTRS)

Cruz, Juan R.; Shah, C. H.; Postyn, A. S.

1996-01-01

In this report, the properties of two carbon-epoxy prepreg materials are presented. The epoxy resin used in these two materials can yield lower manufacturing costs due to its low initial cure temperature, and the capability of being cured using vacuum pressure only. The two materials selected for this study are MR50/LTM25, and CFS003/LTM25 with Amoco T300 fiber; both prepregs are manufactured by The Advanced Composites Group. MR50/LTM25 is a unidirectional prepreg tape using Mitsubishi MR50 carbon fiber impregnated with LTM25 epoxy resin. CRS003/LTM25 is a 2 by 2 twill fabric using Amoco T300 fiber and impregnated with LTM25 epoxy resin. Among the properties presented in this report are strength, stiffness, bolt bearing, and damage tolerance. Many of these properties were obtained at three environmental conditions: cold temperature/dry (CTD), room temperature/dry (RTD), and elevated temperature/wet (ETW). A few properties were obtained at room temperature/wet (RTW), and elevated temperature/dry (ETD). The cold and elevated temperatures used for testing were -125 F and 180 F, respectively. In addition, several properties related to processing are presented.

Nitrogen nutrition and drought hardening exert opposite effects on the stress tolerance of Pinus pinea L. seedlings.

PubMed

Villar-Salvador, Pedro; Peñuelas, Juan L; Jacobs, Douglass F

2013-02-01

Functional attributes determine the survival and growth of planted seedlings in reforestation projects. Nitrogen (N) and water are important resources in the cultivation of forest species, which have a strong effect on plant functional traits. We analyzed the influence of N nutrition on drought acclimation of Pinus pinea L. seedlings. Specifically, we addressed if high N fertilization reduces drought and frost tolerance of seedlings and whether drought hardening reverses the effect of high N fertilization on stress tolerance. Seedlings were grown under two N fertilization regimes (6 and 100 mg N per plant) and subjected to three drought-hardening levels (well-watered, moderate and strong hardening). Water relations, gas exchange, frost damage, N concentration and growth at the end of the drought-hardening period, and survival and growth of seedlings under controlled xeric and mesic outplanting conditions were measured. Relative to low-N plants, high-N plants were larger, had higher stomatal conductance (27%), residual transpiration (11%) and new root growth capacity and closed stomata at higher water potential. However, high N fertilization also increased frost damage (24%) and decreased plasmalemma stability to dehydration (9%). Drought hardening reversed to a great extent the reduction in stress tolerance caused by high N fertilization as it decreased frost damage, stomatal conductance and residual transpiration by 21, 31 and 24%, respectively, and increased plasmalemma stability to dehydration (8%). Drought hardening increased tissue non-structural carbohydrates and N concentration, especially in high-fertilized plants. Frost damage was positively related to the stability of plasmalemma to dehydration (r = 0.92) and both traits were negatively related to the concentration of reducing soluble sugars. No differences existed between moderate and strong drought-hardening treatments. Neither N nutrition nor drought hardening had any clear effect on seedling performance under xeric outplanting conditions. However, fertilization increased growth under mesic conditions, whereas drought hardening decreased growth. We conclude that drought hardening and N fertilization applied under typical container nursery operational conditions exert opposite effects on the physiological stress tolerance of P. pinea seedlings. While drought hardening increases overall stress tolerance, N nutrition reduces it and yet has no effect on the drought acclimation capacity of seedlings.

Root Damage under Alkaline Stress Is Associated with Reactive Oxygen Species Accumulation in Rice (Oryza sativa L.)

PubMed Central

Zhang, Hui; Liu, Xiao-Long; Zhang, Rui-Xue; Yuan, Hai-Yan; Wang, Ming-Ming; Yang, Hao-Yu; Ma, Hong-Yuan; Liu, Duo; Jiang, Chang-Jie; Liang, Zheng-Wei

2017-01-01

Alkaline stress (high pH) severely damages root cells, and consequently, inhibits rice (Oryza sativa L.) seedling growth. In this study, we demonstrate the accumulation of reactive oxygen species (ROS) in root cells under alkaline stress. Seedlings of two rice cultivars with different alkaline tolerances, ‘Dongdao-4’ (moderately alkaline-tolerant) and ‘Jiudao-51’ (alkaline-sensitive), were subjected to alkaline stress simulated by 15 mM sodium carbonate (Na2CO3). Alkaline stress greatly reduced seedling survival rate, shoot and root growth, and root vigor. Moreover, severe root cell damage was observed under alkaline stress, as shown by increased membrane injury, malondialdehyde accumulation, and Evan’s Blue staining. The expression of the cell death-related genes OsKOD1, OsHsr203j, OsCP1, and OsNAC4 was consistently upregulated, while that of a cell death-suppressor gene, OsBI1, was downregulated. Analysis of the ROS contents revealed that alkaline stress induced a marked accumulation of superoxide anions (O2•-) and hydrogen peroxide (H2O2) in rice roots. The application of procyanidins (a potent antioxidant) to rice seedlings 24 h prior to alkaline treatment significantly alleviated alkalinity-induced root damage and promoted seedling growth inhibition, which were concomitant with reduced ROS accumulation. These results suggest that root cell damage, and consequently growth inhibition, of rice seedlings under alkaline stress is closely associated with ROS accumulation. The antioxidant activity of superoxide dismutase, catalase, peroxidase, and ascorbate peroxidase increased under alkaline stress in the roots, probably in response to the cellular damage induced by oxidative stress. However, this response mechanism may be overwhelmed by the excess ROS accumulation observed under stress, resulting in oxidative damage to root cells. Our findings provide physiological insights into the molecular mechanisms of alkalinity-induced damage to root cells, and will contribute to the improvement of alkaline stress tolerance in rice plants. PMID:28943882

Root Damage under Alkaline Stress Is Associated with Reactive Oxygen Species Accumulation in Rice (Oryza sativa L.).

PubMed

Zhang, Hui; Liu, Xiao-Long; Zhang, Rui-Xue; Yuan, Hai-Yan; Wang, Ming-Ming; Yang, Hao-Yu; Ma, Hong-Yuan; Liu, Duo; Jiang, Chang-Jie; Liang, Zheng-Wei

2017-01-01

Alkaline stress (high pH) severely damages root cells, and consequently, inhibits rice ( Oryza sativa L.) seedling growth. In this study, we demonstrate the accumulation of reactive oxygen species (ROS) in root cells under alkaline stress. Seedlings of two rice cultivars with different alkaline tolerances, 'Dongdao-4' (moderately alkaline-tolerant) and 'Jiudao-51' (alkaline-sensitive), were subjected to alkaline stress simulated by 15 mM sodium carbonate (Na 2 CO 3 ). Alkaline stress greatly reduced seedling survival rate, shoot and root growth, and root vigor. Moreover, severe root cell damage was observed under alkaline stress, as shown by increased membrane injury, malondialdehyde accumulation, and Evan's Blue staining. The expression of the cell death-related genes OsKOD1 , OsHsr203j , OsCP1 , and OsNAC4 was consistently upregulated, while that of a cell death-suppressor gene, OsBI1 , was downregulated. Analysis of the ROS contents revealed that alkaline stress induced a marked accumulation of superoxide anions ([Formula: see text]) and hydrogen peroxide (H 2 O 2 ) in rice roots. The application of procyanidins (a potent antioxidant) to rice seedlings 24 h prior to alkaline treatment significantly alleviated alkalinity-induced root damage and promoted seedling growth inhibition, which were concomitant with reduced ROS accumulation. These results suggest that root cell damage, and consequently growth inhibition, of rice seedlings under alkaline stress is closely associated with ROS accumulation. The antioxidant activity of superoxide dismutase, catalase, peroxidase, and ascorbate peroxidase increased under alkaline stress in the roots, probably in response to the cellular damage induced by oxidative stress. However, this response mechanism may be overwhelmed by the excess ROS accumulation observed under stress, resulting in oxidative damage to root cells. Our findings provide physiological insights into the molecular mechanisms of alkalinity-induced damage to root cells, and will contribute to the improvement of alkaline stress tolerance in rice plants.

Micro-Energy Rates for Damage Tolerance and Durability of Composite Structures

NASA Technical Reports Server (NTRS)

Chamis, Christos C.; Minnetyan, Levon

2006-01-01

In this paper, the adhesive bond strength of lap-jointed graphite/aluminum composites is examined by computational simulation. Computed micro-stress level energy release rates are used to identify the damage mechanisms associated with the corresponding acoustic emission (AE) signals. Computed damage regions are similarly correlated with ultrasonically scanned damage regions. Results show that computational simulation can be used with suitable NDE methods for credible in-service monitoring of composites.

Biosentinel: Improving Desiccation Tolerance of Yeast Biosensors for Deep-Space Missions

NASA Technical Reports Server (NTRS)

Dalal, Sawan; Santa Maria, Sergio R.; Liddell, Lauren; Bhattacharya, Sharmila

2017-01-01

BioSentinel is one of 13 secondary payloads to be deployed on Exploration Mission 1 (EM-1) in 2019. We will use the budding yeast Saccharomyces cerevisiae as a biosensor to determine how deep-space radiation affects living organisms and to potentially quantify radiation levels through radiation damage analysis. Radiation can damage DNA through double strand breaks (DSBs), which can normally be repaired by homologous recombination. Two yeast strains will be air-dried and stored in microfluidic cards within the payload: a wild-type control strain and a radiation sensitive rad51 mutant that is deficient in DSB repairs. Throughout the mission, the microfluidic cards will be rehydrated with growth medium and an indicator dye. Growth rates of each strain will be measured through LED detection of the reduction of the indicator dye, which correlates with DNA repair and the amount of radiation damage accumulated. Results from BioSentinel will be compared to analog experiments on the ISS and on Earth. It is well known that desiccation can damage yeast cells and decrease viability over time. We performed a screen for desiccation-tolerant rad51 strains. We selected 20 re-isolates of rad51 and ran a weekly screen for desiccation-tolerant mutants for five weeks. Our data shows that viability decreases over time, confirming previous research findings. Isolates L2, L5 and L14 indicate desiccation tolerance and are candidates for whole-genome sequencing. More time is needed to determine whether a specific strain is truly desiccation tolerant. Furthermore, we conducted an intracellular trehalose assay to test how intracellular trehalose concentrations affect or protect the mutant strains against desiccation stress. S. cerevisiae cell and reagent concentrations from a previously established intracellular trehalose protocol did not yield significant absorbance measurements, so we tested varying cell and reagent concentrations and determined proper concentrations for successful protocol use.

Design Issues for Using Magnetic Materials in Radiation Environments at Elevated Temperature

NASA Technical Reports Server (NTRS)

Bowman, Cheryl L.

2013-01-01

One of the challenges of designing motors and alternators for use in nuclear powered space missions is accounting for the effects of radiation. Terrestrial reactor power plants use distance and shielding to minimize radiation damage but space missions must economize volume and mass. Past studies have shown that sufficiently high radiation levels can affect the magnetic response of hard and soft magnetic materials. Theoretical models explaining the radiation-induced degradation have been proposed but not verified. This paper reviews the literature and explains the cumulative effects of temperature, magnetic-load, and radiation-level on the magnetic properties of component materials. Magnetic property degradation is very specific to alloy choice and processing history, since magnetic properties are very much entwined with specific chemistry and microstructural features. However, there is basic theoretical as well as supportive experimental evidence that the negative impact to magnetic properties will be minimal if the bulk temperature of the material is less than fifty percent of the Curie temperature, the radiation flux is low, and the demagnetization field is small. Keywords: Magnets, Permanent Magnets, Power Converters, Nuclear Electric Power Generation, Radiation Tolerance.

Development of pressure containment and damage tolerance technology for composite fuselage structures in large transport aircraft

NASA Technical Reports Server (NTRS)

Smith, P. J.; Thomson, L. W.; Wilson, R. D.

1986-01-01

NASA sponsored composites research and development programs were set in place to develop the critical engineering technologies in large transport aircraft structures. This NASA-Boeing program focused on the critical issues of damage tolerance and pressure containment generic to the fuselage structure of large pressurized aircraft. Skin-stringer and honeycomb sandwich composite fuselage shell designs were evaluated to resolve these issues. Analyses were developed to model the structural response of the fuselage shell designs, and a development test program evaluated the selected design configurations to appropriate load conditions.

Analysis and Support Initiative for Structural Technology (ASIST) Delivery Order 0016: USAF Damage Tolerant Design Handbook: Guidelines For the Analysis and Design of Damage Tolerant Aircraft Structures

DTIC Science & Technology

2002-11-01

hand crack tip (point B) and with angular displacement from the x-axis. As the stress element is moved closer to the crack tip, the stresses are...on the methods of obtaining the required relationships are presented by Broek [1974]. The necessary relationships for Vσ, VF, Vp and Vst ...4.5.18. Geometrical and Displacement Parameters Relative to the Crack Tip 4.5.21 Vσ + VF + Vp = Vst (4.5.15) substituting the expressions 4.5.6

Acoustic emission monitoring of damage in ceramic matrix composites: Effects of weaves and feature

NASA Astrophysics Data System (ADS)

Ojard, Greg; Mordasky, Matt; Kumar, Rajesh

2018-04-01

Ceramic matrix composites (CMCs) are a class of high temperature materials with better damage tolerance properties compared to monolithic ceramics. The improved toughness is attributed to weak interface coating between the fiber and the matrix that allows for crack deflection and fiber pull-out. Thus, CMCs have gained consideration over monolithic materials for high temperature applications such as in gas turbines. The current standard fiber architecture for CMCs is a harness satin (HS) balanced weave (5HS and 8HS); however, other architectures such as uni-weave materials (tape layup) are now being considered due to fiber placement control and higher fiber volume fraction in the tensile loading direction. Engineering components require additional features in the CMC laminates, such as holes for attachments. Past work has shown that acoustic emission could differentiate the effect of changing interface conditions due to heat treatment effects. The focus of the present work is to investigate the effects of different weaves and the presence of a feature on damage behavior of CMCs as observed via acoustic emission technique. The results of the tensile testing with acoustic emission monitoring will be presented and discussed.

Effect of nickel on point defects diffusion in Fe – Ni alloys

DOE PAGES

Anento, Napoleon; Serra, Anna; Osetsky, Yury N.

2017-05-05

Iron-Nickel alloys are perspective alloys as nuclear energy structural materials because of their good radiation damage tolerance and mechanical properties. Understanding of experimentally observed features such as the effect of Ni content to radiation defects evolution is essential for developing predictive models of radiation. Recently an atomic-scale modelling study has revealed one particular mechanism of Ni effect related to the reduced mobility of clusters of interstitial atoms in Fe-Ni alloys. In this paper we present results of the microsecond-scale molecular dynamics study of point defects, i.e. vacancies and self-interstitial atoms, diffusion in Fe-Ni alloys. It is found that the additionmore » of Ni atoms affects diffusion processes: diffusion of vacancies is enhanced in the presence of Ni, whereas diffusion of interstitials is reduced and these effects increase at high Ni concentration and low temperature. As a result, the role of Ni solutes in radiation damage evolution in Fe-Ni alloys is discussed.« less

Lattice thermal conductivity of multi-component alloys

DOE PAGES

Caro, Magdalena; Béland, Laurent K.; Samolyuk, German D.; ...

2015-06-12

High entropy alloys (HEA) have unique properties including the potential to be radiation tolerant. These materials with extreme disorder could resist damage because disorder, stabilized by entropy, is the equilibrium thermodynamic state. Disorder also reduces electron and phonon conductivity keeping the damage energy longer at the deposition locations, eventually favoring defect recombination. In the short time-scales related to thermal spikes induced by collision cascades, phonons become the relevant energy carrier. In this paper, we perform a systematic study of phonon thermal conductivity in multiple component solid solutions represented by Lennard-Jones (LJ) potentials. We explore the conditions that minimize phonon meanmore » free path via extreme alloy complexity, by varying the composition and the elements (differing in mass, atomic radii, and cohesive energy). We show that alloy complexity can be tailored to modify the scattering mechanisms that control energy transport in the phonon subsystem. Finally, our analysis provides a qualitative guidance for the selection criteria used in the design of HEA alloys with low phonon thermal conductivity.« less

Mechanical behavior of glass/epoxy composite laminate with varying amount of MWCNTs under different loadings

NASA Astrophysics Data System (ADS)

Singh, K. K.; Rawat, Prashant

2018-05-01

This paper investigates the mechanical response of three phased (glass/MWCNTs/epoxy) composite laminate under three different loadings. Flexural strength, short beam strength and low-velocity impact (LVI) testing are performed to find an optimum doping percentage value for maximum enhancement in mechanical properties. In this work, MWCNTs were used as secondary reinforcement for three-phased composite plate. MWCNT doping was done in a range of 0–4 wt% of the thermosetting matrix system. Symmetrical design eight layered glass/epoxy laminate with zero bending extension coupling laminate was fabricated using a hybrid method i.e. hand lay-up technique followed by vacuum bagging method. Ranging analysis of MWCNT mixing highlighted the enhancement in flexural, short beam strength and improvement in damage tolerance under LVI loading. While at higher doping wt%, agglomeration of MWCNTs are observed. Results of mechanical testing proposed an optimized doping value for maximum strength and damage resistance of the laminate.

Stimulation of the penetration of particles into the skin by plasma tissue interaction

NASA Astrophysics Data System (ADS)

Lademann, O.; Richter, H.; Kramer, A.; Patzelt, A.; Meinke, M. C.; Graf, C.; Gao, Q.; Korotianskiy, E.; Rühl, E.; Weltmann, K.-D.; Lademann, J.; Koch, S.

2011-10-01

A high number of treatments in dermatology are based on the penetration of topically applied drugs through the skin barrier. This process is predominantly inefficient, on account of the strong protection properties of the upper skin layer - the stratum corneum. If the skin barrier is damaged, the penetration efficiency of topically applied drugs increases. Therefore, different methods have been developed to influence the barrier properties of the skin. Recently, it could be demonstrated that a cold tissue tolerable plasma (TTP) produced by a plasma-jet can strongly enhance drug delivery through the skin. These investigations were performed by using a solution of fluorescent dye as a model drug. In the present study, these investigations were carried out using fluorescent silica particles at different sizes. The aim of the study was to investigate whether or not there is a limitation in size for topically applied substances to pass through the skin barrier after plasma treatment.

Rheological and fracturing characteristics of a novel sulfonated hydroxypropyl guar gum.

PubMed

Qiu, Liewei; Shen, Yiding; Wang, Tao; Wang, Chen

2018-05-15

A series of sulfonated hydroxypropyl guar gum (SHG) samples with different degrees of substitution (DSs) were prepared, and the SHG solution and SHG fracturing fluid were prepared and analyzed. The SHG aqueous solutions with different DSs all exhibit shear thinning behavior, which is well correlated with the Ostwald-deWaele model. Owing to the electrostatic repulsion of SHG molecular chains, SHG solutions with a higher DS will exhibit weaker thixotropic performance and strong anti-salinity ability. In addition, the SHG fracturing fluids, which were formed by interactions between SHG and organic zirconium, exhibit good temperature- and shear-resistant properties, proppant suspension properties, and salt tolerance. Furthermore, SHG gel-breaking fluids show low interfacial and surface tensions, with low residue content and small core permeability damage. These results provide useful indicators for the applications of SHG in the oil field industry. Copyright © 2017. Published by Elsevier B.V.

A designed experiment in stitched/RTM composites

NASA Technical Reports Server (NTRS)

Dickinson, Larry C.

1993-01-01

The damage tolerance of composite laminates can be significantly improved by the addition of through-the-thickness fibrous reinforcement such as stitching. However, there are numerous stitching parameters which can be independently varied, and their separate and combined effects on mechanical properties need to be determined. A statistically designed experiment (a 2(sup 5-1) fractional factorial, also known as a Taguchi L16 test matrix) used to evaluate five important parameters is described. The effects and interactions of stitch thread material, stitch thread strength, stitch row spacing and stitch pitch are examined for both thick (48 ply) and thin (16 ply) carbon/epoxy (AS4/E905L) composites. Tension, compression and compression after impact tests are described. Preliminary results of completed tension testing are discussed. Larger threads decreased tensile strength. Panel thickness was found not to be an important stitching parameter for tensile properties. Tensile modulus was unaffected by stitching.

[Immunomodulatory properties of stem mesenchymal cells in autoimmune diseases].

PubMed

Sánchez-Berná, Isabel; Santiago-Díaz, Carlos; Jiménez-Alonso, Juan

2015-01-20

Autoimmune diseases are a cluster of disorders characterized by a failure of the immune tolerance and a hyperactivation of the immune system that leads to a chronic inflammation state and the damage of several organs. The medications currently used to treat these diseases usually consist of immunosuppressive drugs that have significant systemic toxic effects and are associated with an increased risk of opportunistic infections. Recently, several studies have demonstrated that mesenchymal stem cells have immunomodulatory properties, a feature that make them candidates to be used in the treatment of autoimmune diseases. In the present study, we reviewed the role of this therapy in the treatment of systemic lupus erythematosus, Sjögren's syndrome, systemic sclerosis, Crohn's disease and multiple sclerosis, as well as the potential risks associated with its use. Copyright © 2013 Elsevier España, S.L.U. All rights reserved.

Formation Mechanisms, Structure, and Properties of HVOF-Sprayed WC-CoCr Coatings: An Approach Toward Process Maps

NASA Astrophysics Data System (ADS)

Varis, T.; Suhonen, T.; Ghabchi, A.; Valarezo, A.; Sampath, S.; Liu, X.; Hannula, S.-P.

2014-08-01

Our study focuses on understanding the damage tolerance and performance reliability of WC-CoCr coatings. In this paper, the formation of HVOF-sprayed tungsten carbide-based cermet coatings is studied through an integrated strategy: First-order process maps are created by using online-diagnostics to assess particle states in relation to process conditions. Coating properties such as hardness, wear resistance, elastic modulus, residual stress, and fracture toughness are discussed with a goal to establish a linkage between properties and particle characteristics via second-order process maps. A strong influence of particle state on the mechanical properties, wear resistance, and residual stress stage of the coating was observed. Within the used processing window (particle temperature ranged from 1687 to 1831 °C and particle velocity from 577 to 621 m/s), the coating hardness varied from 1021 to 1507 HV and modulus from 257 to 322 GPa. The variation in coating mechanical state is suggested to relate to the microstructural changes arising from carbide dissolution, which affects the properties of the matrix and, on the other hand, cohesive properties of the lamella. The complete tracking of the coating particle state and its linking to mechanical properties and residual stresses enables coating design with desired properties.

Loss of desiccation tolerance in Copaifera langsdorffii Desf. seeds during germination.

PubMed

Pereira, W V S; Faria, J M R; Tonetti, O A O; Silva, E A A

2014-05-01

This study evaluated the loss of desiccation tolerance in C. langsdorffii seeds during the germination process. Seeds were imbibed for 24, 48, 72, 96, 120 and 144 hours and dried to the initial moisture content, kept in this state for 3 days after which they were submitted to pre-humidification and rehydration. Ultraestructural evaluations were done aiming to observe the cell damage caused by the dry process. Desiccation tolerance was evaluated in terms of the percentage of normal seedlings. Seeds not submitted to the drying process presented 61% of normal seedlings, and after 24 hours of imbibition, followed by drying, the seeds presented the same percentage of survival. However, after 48 hours of imbibition, seeds started to lose the desiccation tolerance. There was twenty six percent of normal seedlings formed from seeds imbibed for 96 hours and later dried and rehydrated. Only 5% of seeds imbibed for 144 hours, dried and rehydrated formed normal seedlings. At 144 hours of imbibition followed the dry process, there was damage into the cell structure, indicating that the seeds were unable to keep the cell structure during the drying process. Copaifera langsdorffii seeds loses the desiccation tolerance at the start of Phase 2 of imbibition.

ALA Pretreatment Improves Waterlogging Tolerance of Fig Plants

PubMed Central

An, Yuyan; Qi, Lin; Wang, Liangju

2016-01-01

5-aminolevulinic acid (ALA), a natural and environmentally friendly plant growth regulator, can improve plant tolerance to various environmental stresses. However, whether ALA can improve plant waterlogging tolerance is unknown. Here, we investigated the effects of ALA pretreatment on the waterlogging-induced damage of fig (Ficus carica Linn.) plants, which often suffer from waterlogging stress. ALA pretreatment significantly alleviated stress-induced morphological damage, increased leaf relative water content (RWC), and reduced leaf superoxide anion (O2⋅¯) production rate and malonaldehyde (MDA) content in fig leaves, indicating ALA mitigates waterlogging stress of fig plants. We further demonstrated that ALA pretreatment largely promoted leaf chlorophyll content, photosynthetic electron transfer ability, and photosynthetic performance index, indicating ALA significantly improves plant photosynthetic efficiency under waterlogging stress. Moreover, ALA pretreatment significantly increased activities of leaf superoxide dismutase (SOD) and peroxidase (POD), root vigor, and activities of root alcohol dehydrogenase (ADH), and lactate dehydrogenase (LDH), indicating ALA also significantly improves antioxidant ability and root function of fig plants under waterlogging stress. Taken together, ALA pretreatment improves waterlogging tolerance of fig plants significantly, and the promoted root respiration, leaf photosynthesis, and antioxidant ability may contribute greatly to this improvement. Our data firstly shows that ALA can improve plant waterlogging tolerance. PMID:26789407

77 FR 42547 - Agency Information Collection Activities: Requests for Comments; Clearance of Renewed Approval of...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-19

... and Fatigue Evaluation of Composite Rotorcraft Structures AGENCY: Federal Aviation Administration (FAA... Tolerance and Fatigue Evaluation of Composite Rotorcraft Structures. Form Numbers: There are no FAA forms... ``Damage Tolerance and Fatigue Evaluation of Composite Rotorcraft Structures'' final rule (76 FR 74655...

Tough, processable simultaneous semi-interpenetrating polyimides

NASA Technical Reports Server (NTRS)

Pater, Ruth H. (Inventor)

1996-01-01

A high temperature semi-interpenetrating polymer network (semi-IPN) was developed which had significantly improved processability, damage tolerance and mechanical performance, when compared to the commercial Thermid.RTM. materials. This simultaneous semi-IPN was prepared by mixing a thermosetting polyimide with a thermoplastic monomer precursor solution (NR-15082) and allowing them to react upon heating. This reaction occurs at a rate which decreases the flow and broadens the processing window. Upon heating at a higher temperature, there is an increase in flow. Because of the improved flow properties, broadened processing window and enhanced toughness, high strength polymer matrix composites, adhesives and molded articles can now be prepared from the acetylene endcapped polyimides which were previously inherently brittle and difficult to process.

Recent Advances in Composite Damage Mechanics

NASA Technical Reports Server (NTRS)

Reifsnider, Ken; Case, Scott; Iyengar, Nirmal

1996-01-01

The state of the art and recent developments in the field of composite material damage mechanics are reviewed, with emphasis on damage accumulation. The kinetics of damage accumulation are considered with emphasis on the general accumulation of discrete local damage events such as single or multiple fiber fractures or microcrack formation. The issues addressed include: how to define strength in the presence of widely distributed damage, and how to combine mechanical representations in order to predict the damage tolerance and life of engineering components. It is shown that a damage mechanics approach can be related to the thermodynamics of the damage accumulation processes in composite laminates subjected to mechanical loading and environmental conditions over long periods of time.

Impact Damage and Strain Rate Effects for Toughened Epoxy Composite Structures

NASA Technical Reports Server (NTRS)

Chamis, Christos C.; Minnetyan, Levon

2006-01-01

Structural integrity of composite systems under dynamic impact loading is investigated herein. The GENOA virtual testing software environment is used to implement the effects of dynamic loading on fracture progression and damage tolerance. Combinations of graphite and glass fibers with a toughened epoxy matrix are investigated. The effect of a ceramic coating for the absorption of impact energy is also included. Impact and post impact simulations include verification and prediction of (1) Load and Impact Energy, (2) Impact Damage Size, (3) Maximum Impact Peak Load, (4) Residual Strength, (5) Maximum Displacement, (6) Contribution of Failure Modes to Failure Mechanisms, (7) Prediction of Impact Load Versus Time, and (8) Damage, and Fracture Pattern. A computer model is utilized for the assessment of structural response, progressive fracture, and defect/damage tolerance characteristics. Results show the damage progression sequence and the changes in the structural response characteristics due to dynamic impact. The fundamental premise of computational simulation is that the complete evaluation of composite fracture requires an assessment of ply and subply level damage/fracture processes as the structure is subjected to loads. Simulation results for the graphite/epoxy composite were compared with the impact and tension failure test data, correlation and verification was obtained that included: (1) impact energy, (2) damage size, (3) maximum impact peak load, (4) residual strength, (5) maximum displacement, and (6) failure mechanisms of the composite structure.

Structural Design Methodology Based on Concepts of Uncertainty

NASA Technical Reports Server (NTRS)

Lin, K. Y.; Du, Jiaji; Rusk, David

2000-01-01

In this report, an approach to damage-tolerant aircraft structural design is proposed based on the concept of an equivalent "Level of Safety" that incorporates past service experience in the design of new structures. The discrete "Level of Safety" for a single inspection event is defined as the compliment of the probability that a single flaw size larger than the critical flaw size for residual strength of the structure exists, and that the flaw will not be detected. The cumulative "Level of Safety" for the entire structure is the product of the discrete "Level of Safety" values for each flaw of each damage type present at each location in the structure. Based on the definition of "Level of Safety", a design procedure was identified and demonstrated on a composite sandwich panel for various damage types, with results showing the sensitivity of the structural sizing parameters to the relative safety of the design. The "Level of Safety" approach has broad potential application to damage-tolerant aircraft structural design with uncertainty.

An assessment of buffer strips for improving damage tolerance of composite laminates at elevated temperature

NASA Technical Reports Server (NTRS)

Bigelow, C. A.

1981-01-01

Buffer strips greatly improve the damage tolerance of graphite/epoxy laminates loaded in tension. Graphite/polyimide buffer strip panels were made and tested to determine their residual strength at ambient and elevated (177 C) temperature. Each panel was cut in the center to represent damage. Panels were radiographed and crack-opening displacements were recorded to indicate fracture, fracture arrest, and the extent of damage in the buffer strip after arrest. All panels had the same buffer strip spacing and width. The buffer strip material was 0 deg S-glass/PMR-15. The buffer strips were made by replacing narrow strips of the 0 deg graphite plies with strips of the 0 deg S-glass on either a one-for-one or a two-for-one basis. Half of the panels were heated to 177 + or - 3 C before and during the testing. Elevated temperature did not alter the fracture behavior of the buffer configuration.

Towards a damage tolerance philosophy for composite materials and structures

NASA Technical Reports Server (NTRS)

O'Brien, T. Kevin

1990-01-01

A damage-threshold/fail-safe approach is proposed to ensure that composite structures are both sufficiently durable for economy of operation, as well as adequately fail-safe or damage tolerant for flight safety. Matrix cracks are assumed to exist throughout the off-axis plies. Delamination onset is predicted using a strain energy release rate characterization. Delamination growth is accounted for in one of three ways: either analytically, using delamination growth laws in conjunction with strain energy release rate analyses incorporating delamination resistance curves; experimentally, using measured stiffness loss; or conservatively, assuming delamination onset corresponds to catastrophic delamination growth. Fail-safety is assessed by accounting for the accumulation of delaminations through the thickness. A tension fatigue life prediction for composite laminates is presented as a case study to illustrate how this approach may be implemented. Suggestions are made for applying the damage-threshold/fail-safe approach to compression fatigue, tension/compression fatigue, and compression strength following low velocity impact.

Towards a damage tolerance philosophy for composite materials and structures

NASA Technical Reports Server (NTRS)

Obrien, T. Kevin

1988-01-01

A damage-threshold/fail-safe approach is proposed to ensure that composite structures are both sufficiently durable for economy of operation, as well as adequately fail-safe or damage tolerant for flight safety. Matrix cracks are assumed to exist throughout the off-axis plies. Delamination onset is predicted using a strain energy release rate characterization. Delamination growth is accounted for in one of three ways: either analytically, using delamination growth laws in conjunction with strain energy release rate analyses incorporating delamination resistance curves; experimentally, using measured stiffness loss; or conservatively, assuming delamination onset corresponds to catastrophic delamination growth. Fail-safety is assessed by accounting for the accumulation of delaminations through the thickness. A tension fatigue life prediction for composite laminates is presented as a case study to illustrate how this approach may be implemented. Suggestions are made for applying the damage-threshold/fail-safe approach to compression fatigue, tension/compression fatigue, and compression strength following low velocity impact.

Assessing inspection sensitivity as it relates to damage tolerance in composite rotor hubs

NASA Astrophysics Data System (ADS)

Roach, Dennis P.; Rackow, Kirk

2001-08-01

Increasing niche applications, growing international markets, and the emergence of advanced rotorcraft technology are expected to greatly increase the population of helicopters over the next decade. In terms of fuselage fatigue, helicopters show similar trends as fixed-wing aircraft. The highly unsteady loads experienced by rotating wings not only directly affect components in the dynamic systems but are also transferred to the fixed airframe structure. Expanded use of rotorcraft has focused attention on the use of new materials and the optimization of maintenance practices. The FAA's Airworthiness Assurance Center (AANC) at Sandia National Labs has joined with Bell Helicopter andother agencies in the rotorcraft industry to evaluate nondestructive inspection (NDI) capabilities in light of the damage tolerance of assorted rotorcraft structure components. Currently, the program's emphasis is on composite rotor hubs. The rotorcraft industry is constantly evaluating new types of lightweight composite materials that not only enhance the safety and reliability of rotor components but also improve performance and extended operating life as well. Composite rotor hubs have led to the use of bearingless rotor systems that are less complex and require less maintenance than their predecessors. The test facility described in this paper allows the structural stability and damage tolerance of composite hubs to be evaluated using realistic flight load spectrums of centrifugal force and bending loads. NDI was integrated into the life-cycle fatigue tests in order to evaluate flaw detection sensitivity simultaneously wiht residual strength and general rotor hub peformance. This paper will describe the evolving use of damage tolerance analysis (DTA) to direct and improve rotorcraft maintenance along with the related use of nondestructive inspections to manage helicopter safety. OVeralll, the data from this project will provide information to improve the producibility, inspectability, serviceability, and cost effectively of rotorcraft components.

Distribution of pines in the Iberian Peninsula agrees with species differences in foliage frost tolerance, not with vulnerability to freezing-induced xylem embolism.

PubMed

Fernández-Pérez, Laura; Villar-Salvador, Pedro; Martínez-Vilalta, Jordi; Toca, Andrei; Zavala, Miguel A

2018-04-01

Drought and frosts are major determinants of plant functioning and distribution. Both stresses can cause xylem embolism and foliage damage. The objective of this study was to analyse if the distribution of six common pine species along latitudinal and altitudinal gradients in Europe is related to their interspecific differences in frost tolerance and to the physiological mechanisms underlying species-specific frost tolerance. We also evaluate if frost tolerance depends on plant water status. We studied survival to a range of freezing temperatures in 2-year-old plants and assessed the percentage loss of hydraulic conductivity (PLC) due xylem embolism formation and foliage damage determined by needle electrolyte leakage (EL) after a single frost cycle to -15 °C and over a range of predawn water potential (ψpd) values. Species experiencing cold winters in their range (Pinus nigra J.F. Arnold, Pinus sylvestris L. and Pinus uncinata Raymond ex A. DC.) had the highest frost survival rates and lowest needle EL and soluble sugar (SS) concentration. In contrast, the pines inhabiting mild or cool winter locations (especially Pinus halepensis Mill. and Pinus pinea L. and, to a lesser extent, Pinus pinaster Ait.) had the lowest frost survival and highest needle EL and SS values. Freezing-induced PLC was very low and differences among species were not related to frost damage. Reduction in ψpd decreased leaf frost damage in P. pinea and P. sylvestris, increased it in P. uncinata and had a neutral effect on the rest of the species. This study demonstrates that freezing temperatures are a major environmental driver for pine distribution and suggests that interspecific differences in leaf frost sensitivity rather than vulnerability to freezing-induced embolism or SS explain pine juvenile frost survival.

Plant compensatory growth in aspen seedlings: the role of frequency and intensity of herbivory and resource availability

Treesearch

Nadir Erbilgin; David A. Galvez; Bin Zhang

2012-01-01

Plant ecologists have debated the mechanisms used by plants to cope with the impact of herbivore damage for more than a century. During that time, plant resistance mechanisms, which reduce the amount of herbivore damage before and during herbivory, have received most of the attention, while plant tolerance mechanisms, which may minimize the impacts of damage after...

Crack Detection in Fibre Reinforced Plastic Structures Using Embedded Fibre Bragg Grating Sensors: Theory, Model Development and Experimental Validation

PubMed Central

Pereira, G. F.; Mikkelsen, L. P.; McGugan, M.

2015-01-01

In a fibre-reinforced polymer (FRP) structure designed using the emerging damage tolerance and structural health monitoring philosophy, sensors and models that describe crack propagation will enable a structure to operate despite the presence of damage by fully exploiting the material’s mechanical properties. When applying this concept to different structures, sensor systems and damage types, a combination of damage mechanics, monitoring technology, and modelling is required. The primary objective of this article is to demonstrate such a combination. This article is divided in three main topics: the damage mechanism (delamination of FRP), the structural health monitoring technology (fibre Bragg gratings to detect delamination), and the finite element method model of the structure that incorporates these concepts into a final and integrated damage-monitoring concept. A novel method for assessing a crack growth/damage event in fibre-reinforced polymer or structural adhesive-bonded structures using embedded fibre Bragg grating (FBG) sensors is presented by combining conventional measured parameters, such as wavelength shift, with parameters associated with measurement errors, typically ignored by the end-user. Conjointly, a novel model for sensor output prediction (virtual sensor) was developed using this FBG sensor crack monitoring concept and implemented in a finite element method code. The monitoring method was demonstrated and validated using glass fibre double cantilever beam specimens instrumented with an array of FBG sensors embedded in the material and tested using an experimental fracture procedure. The digital image correlation technique was used to validate the model prediction by correlating the specific sensor response caused by the crack with the developed model. PMID:26513653

QTL analysis of frost damage in pea suggests different mechanisms involved in frost tolerance.

PubMed

Klein, Anthony; Houtin, Hervé; Rond, Céline; Marget, Pascal; Jacquin, Françoise; Boucherot, Karen; Huart, Myriam; Rivière, Nathalie; Boutet, Gilles; Lejeune-Hénaut, Isabelle; Burstin, Judith

2014-06-01

Avoidance mechanisms and intrinsic resistance are complementary strategies to improve winter frost tolerance and yield potential in field pea. The development of the winter pea crop represents a major challenge to expand plant protein production in temperate areas. Breeding winter cultivars requires the combination of freezing tolerance as well as high seed productivity and quality. In this context, we investigated the genetic determinism of winter frost tolerance and assessed its genetic relationship with yield and developmental traits. Using a newly identified source of frost resistance, we developed a population of recombinant inbred lines and evaluated it in six environments in Dijon and Clermont-Ferrand between 2005 and 2010. We developed a genetic map comprising 679 markers distributed over seven linkage groups and covering 947.1 cM. One hundred sixty-one quantitative trait loci (QTL) explaining 9-71 % of the phenotypic variation were detected across the six environments for all traits measured. Two clusters of QTL mapped on the linkage groups III and one cluster on LGVI reveal the genetic links between phenology, morphology, yield-related traits and frost tolerance in winter pea. QTL clusters on LGIII highlighted major developmental gene loci (Hr and Le) and the QTL cluster on LGVI explained up to 71 % of the winter frost damage variation. This suggests that a specific architecture and flowering ideotype defines frost tolerance in winter pea. However, two consistent frost tolerance QTL on LGV were independent of phenology and morphology traits, showing that different protective mechanisms are involved in frost tolerance. Finally, these results suggest that frost tolerance can be bred independently to seed productivity and quality.

Impact Characteristics of Candidate Materials for Single-Stage-to-Orbit (SSTO) Technology

NASA Technical Reports Server (NTRS)

Nettles, Alan

1995-01-01

Four fiber/resin systems were compared for resistance to damage and damage tolerance. One toughened epoxy and three toughened bismaleimide (BMI) resins were used., all with IM7 carbon fiber reinforcement. A statistical design of experiments technique was used to evaluate the effects of impact energy, specimen thickness and tup diameter on the damage area and residual compression-after-impact (CAI) strength. Results showed that two of the BMI systems sustained relatively large damage areas yet had an excellent retention of CAI strength.

Quantitative nondestructive evaluation of materials and structures

NASA Technical Reports Server (NTRS)

Smith, Barry T.

1991-01-01

An experimental investigation was undertaken to quantify damage tolerance and resistance in composite materials impacted using the drop-weight method. Tests were conducted on laminates of several different carbon-fiber composite systems, such as epoxies, modified epoxies, and amorphous and semicrystalline thermoplastics. Impacted composite specimens were examined using destructive and non-destructive techniques to establish the characteristic damage states. Specifically, optical microscopy, ultrasonic, and scanning electron microscopy techniques were used to identify impact induced damage mechanisms. Damage propagation during post impact compression was also studied.

In situ heavy ion irradiation studies of nanopore shrinkage and enhanced radiation tolerance of nanoporous Au

DOE PAGES

Li, Jin; Fan, Cuncai; Ding, Jie; ...

2017-01-03

High energy particle radiations induce severe microstructural damage in metallic materials. Nanoporous materials with a giant surface-to-volume ratio may alleviate radiation damage in irradiated metallic materials as free surface are defect sinks. We show, by using in situ Kr ion irradiation in a transmission electron microscope at room temperature, that nanoporous Au indeed has significantly improved radiation tolerance comparing with coarse-grained, fully dense Au. In situ studies show that nanopores can absorb and eliminate a large number of radiation-induced defect clusters. Meanwhile, nanopores shrink (self-heal) during radiation, and their shrinkage rate is pore size dependent. Furthermore, the in situ studiesmore » show dose-rate-dependent diffusivity of defect clusters. Our study sheds light on the design of radiation-tolerant nanoporous metallic materials for advanced nuclear reactor applications.« less

Lower Length Scale Characterization and Validation of Formation and Stability of Helium Bubbles in Nano-structured Ferritic Alloys under Irradiation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhao, Huijuan; Yun, Di; Hoelzer, David

In order to extend the operating license of current light water reactors (LWRs) in the United States and other countries to as many as 80 years or longer, it is demanding to identify potential materials for many of the internal structural components and fasteners. We proposed that 14YWT iron alloy can be adopted in such applications with its excellent material properties, such as high-temperature strength, low creep rate, and high irradiation resistance. Application with 14YWT would improve the void/helium swelling characteristics of the LWR fuels, extend the burn-up limits with the tolerant temperature up to 800oC and reduce the hydrogenmore » production. One key feature of 14YWT material property enhancement is the ultrafine high density of 2-4nm Y-Ti-O enriched nanoclusters (NCs) within the 14YWT iron matrix. The NCs can effectively pin the ultra-fine grain boundaries and dislocations, which significantly enhance mechanical properties of the alloy. Moreover, these nanoclusters remain stable with no coarsening after a large dose of ion irradiation. After ion irradiation, the helium bubbles are observed extremely uniform in size (1nm) and quite homogeneously distributed within the 14YWT matrix, which indicates that the microstructure of 14YWT remains remarkably tolerance to radiation damage. However, there is a lack of understanding of 14YWT both theoretically and experimentally in order to understand the mechanism behind the material property enhancement and to further develop and design a new generation of advanced structural material for current LWR applications and future fusion applications.« less

Error Mitigation of Point-to-Point Communication for Fault-Tolerant Computing

NASA Technical Reports Server (NTRS)

Akamine, Robert L.; Hodson, Robert F.; LaMeres, Brock J.; Ray, Robert E.

2011-01-01

Fault tolerant systems require the ability to detect and recover from physical damage caused by the hardware s environment, faulty connectors, and system degradation over time. This ability applies to military, space, and industrial computing applications. The integrity of Point-to-Point (P2P) communication, between two microcontrollers for example, is an essential part of fault tolerant computing systems. In this paper, different methods of fault detection and recovery are presented and analyzed.

Registration of an oilseed sunflower germplasm line HA-BSR1 highly tolerant to Sclerotinia basal stalk rot

USDA-ARS?s Scientific Manuscript database

Basal stalk rot (BSR) caused by Sclerotinia sclerotiorum (Lib.) de Bary is a devastating disease that causes a significant damage to worldwide sunflower (Helianthus annuus L.) production by reducing seed yield and quality. The objective of this research was to develop highly BSR tolerant sunflower g...

Exploring heteroplasmy as the basis for maternally-transmitted cold tolerance in cucumber

USDA-ARS?s Scientific Manuscript database

Cucumber is a warm-season crop that can be severely damaged by short periods of cold temperatures. Growers would benefit from cold tolerant cucumbers by preventing crop loss in inclement weather as well as by allowing for earlier planting and harvest, thus avoiding heavy late-season disease pressure...

Tolerance as a potential control method for Hessian fly (Diptera:Cecidomyiidae) in winter wheat.

USDA-ARS?s Scientific Manuscript database

Tolerance in wheat may hold the key to reducing damage caused by the Hessian fly, Mayetiola destructor, while enabling the plant to grow normally and reducing the selection pressures leading to increased virulence in fly populations. The susceptible lines Pioneer 25R75, susceptible wheat cultivar ‘N...

Stress-tolerance of baker's-yeast (Saccharomyces cerevisiae) cells: stress-protective molecules and genes involved in stress tolerance.

PubMed

Shima, Jun; Takagi, Hiroshi

2009-05-29

During the fermentation of dough and the production of baker's yeast (Saccharomyces cerevisiae), cells are exposed to numerous environmental stresses (baking-associated stresses) such as freeze-thaw, high sugar concentrations, air-drying and oxidative stresses. Cellular macromolecules, including proteins, nucleic acids and membranes, are seriously damaged under stress conditions, leading to the inhibition of cell growth, cell viability and fermentation. To avoid lethal damage, yeast cells need to acquire a variety of stress-tolerant mechanisms, for example the induction of stress proteins, the accumulation of stress protectants, changes in membrane composition and repression of translation, and by regulating the corresponding gene expression via stress-triggered signal-transduction pathways. Trehalose and proline are considered to be critical stress protectants, as is glycerol. It is known that these molecules are effective for providing protection against various types of environmental stresses. Modifications of the metabolic pathways of trehalose and proline by self-cloning methods have significantly increased tolerance to baking-associated stresses. To clarify which genes are required for stress tolerance, both a comprehensive phenomics analysis and a functional genomics analysis were carried out under stress conditions that simulated those occurring during the commercial baking process. These analyses indicated that many genes are involved in stress tolerance in yeast. In particular, it was suggested that vacuolar H+-ATPase plays important roles in yeast cells under stress conditions.

Induction of cross-tolerance between protective effect of morphine and nicotine in 6-hydroxydopamine-induce neurotoxicity in SH-SY5Y human dopaminergic neuroblastoma cells.

PubMed

Elyasi, Leila; Eftekhar-Vaghefi, Seyed Hassan; Asadi-Shekaaria, Majid; Esmaeili-Mahani, Saeed

2018-06-27

Parkinson's disease is a progressive neurodegenerative disease characterized by progressive and selective death of dopaminergic neurons. It has been reported that nicotine and morphine have protective roles during neuronal damage in Parkinson's disease. In addition, the induction of cross-tolerance between their biological effects has been shown in numerous reports. Here, we investigated the effects of nicotine and morphine on 6-OHDA-induced neurotoxicity in human neuroblastoma SH-SY5Y cell line as an in vitro model of Parkinson's disease. Cell damage was induced by 150 μM 6-OHDA and the cells viability was examined by MTT assay. Intracellular reactive oxygen species, calcium level and mitochondrial membrane potential were determined by fluorescence spectrophotometer method. Biochemical markers of apoptosis were also evaluated by immunoblotting. The data showed that morphine and nicotine prevent 6-OHDA- induced cell damage and apoptosis. However, the protective effects of nicotine were not observed in chronic morphine-pretreated cells. Morphine had no protective effects in chronic nicotine-incubated cells. A cross-tolerance between protective effects of morphine and nicotine was occurred in 6-OHDA-induced SH-SY5Y cell toxicity.

Turtle anoxia tolerance: Biochemistry and gene regulation.

PubMed

Krivoruchko, Anastasia; Storey, Kenneth B

2015-06-01

While oxygen limitation can be extremely damaging for many animals, some vertebrates have perfected anaerobic survival. Freshwater turtles belonging to the Trachemys and Chrysemys genera, for example, can survive many weeks without oxygen, and as such are commonly used as model animals for vertebrate anoxia tolerance. In the present review we discuss the recent advances made in understanding the biochemical and molecular nature of natural anoxia tolerance of freshwater turtles. Research in recent years has shown that activation of several important pathways occurs in response to anoxia in turtles, including those that function in the stress response, cell cycle arrest, inhibition of gene expression and metabolism. These likely contribute to anoxia tolerance in turtle tissues by minimizing cell damage in response to anoxia, as well as facilitating metabolic rate depression. The research discussed in the present review contributes to the understanding of how freshwater turtles can survive without oxygen for prolonged periods of time. This could also improve understanding of the molecular nature of hypoxic/ischemic injuries in mammalian tissues and suggest potential ways to avoid these. Copyright © 2015 Elsevier B.V. All rights reserved.

Effect of zinc and lead on the physiological and biochemical properties of aquatic plant Lemna minor: its potential role in phytoremediation

NASA Astrophysics Data System (ADS)

Jayasri, M. A.; Suthindhiran, K.

2017-06-01

Plants have gained importance in situ bioremediation of heavy metals. In the present study, different concentrations of zinc (Zn2+) (0.5, 5, 10, 15, 20 mg/l) and lead (Pb2+) (1, 2, 4, 6, 8 mg/l) were used to evaluate metal tolerance level of Lemna minor. L.minor were exposed to metals for 4 days and tested for its dry to fresh weight ratio (DW/FW), photosynthetic pigments production and protein content. The oxidative damage was detected by measuring catalase activity. L.minor showed tolerance against Zn2+ and Pb2+ at a concentration of 10 and 4 mg/l, respectively. Among the metals, Pb2+ showed a significant toxicity at 8 mg/l. High concentration (20 mg/l of Zn2+ and 8 mg/l of Pb2+) of the metals displayed a considerable negative effect on soluble proteins (13 fold decrease with Zn2+ and 4 fold decrease with Pb2+) and photosynthetic pigments (twofold decrease with Zn2+ and onefold decrease with Pb2+) and lead to a consequent reduction in number of fronds. Further, the catalase was greatly increased (twofold decrease with Zn2+ and sixfold decrease with Pb2+) under metal stress. The results indicate that L.minor withstands Zn2+ and Pb2+ toxicity up to the concentration of 10 and 4 mg/l, respectively. Hence, the metal tolerant property of this plant shall be exploited for bioremediation of Zinc and Lead in polluted water. Further, the detailed and wide range of heavy metal toxicity studies should be done to reveal the possible use of this plant on large scale bioremediation purpose.

Hurricane Katrina winds damaged longleaf pine less than loblolly pine

Treesearch

Kurt H. Johnsen; John R. Butnor; John S. Kush; Ronald C. Schmidtling; C. Dana Nelson

2009-01-01

Some evidence suggests that longleaf pine might be more tolerant of high winds than either slash pine (Pinus elliotii Englem.) or loblolly pine (Pinus taeda L.). We studied wind damage to these three pine species in a common garden experiment in southeast Mississippi following Hurricane Katrina,...

Beauveria bassiana, Metarhizium anisopliae, and Metarhizium anisopliae var. acridum conidia: tolerance to imbibitional damage

USDA-ARS?s Scientific Manuscript database

When dry fungal cells are immersed in water, rapid imbibition (water uptake) may compromise the plasma membrane, killing the cell. This study investigated the impact of imbibitional damage (measured in terms of reduced viability) on Beauveria bassiana (Bb), Metarhizium anisopliae (Ma) and M. anisop...

7 CFR 51.1861 - Tolerances.

Code of Federal Regulations, 2014 CFR

2014-01-01

...; (ii) Ten percent for tomatoes which are damaged by shoulder bruises or by discolored or sunken scars... affected by decay; (ii) Ten percent for tomatoes which are seriously damaged by shoulder bruises or by... shoulder bruises or by discolored or sunken scars on any parts of the tomatoes; and, (iii) Ten percent for...

7 CFR 51.1861 - Tolerances.

Code of Federal Regulations, 2013 CFR

2013-01-01

...; (ii) Ten percent for tomatoes which are damaged by shoulder bruises or by discolored or sunken scars... affected by decay; (ii) Ten percent for tomatoes which are seriously damaged by shoulder bruises or by... shoulder bruises or by discolored or sunken scars on any parts of the tomatoes; and, (iii) Ten percent for...

Influence of G-forces and hypodynamia on the portal system of the liver

NASA Technical Reports Server (NTRS)

Drozdova, A. V.

1975-01-01

Longitudinally and transversely applied g-forces as intolerable levels cause damage to the interlobular veins of the liver, with maximum damage caused by dorso-ventral g-force application. The portal vein system is more resilient with respect to g-forces at the limit of tolerance.

Recent Advances in Thermoplastic Puncture-Healing Polymers

NASA Technical Reports Server (NTRS)

Bogert, Philip B.; Working, Dennis C.; Wise, Kristopher E.; Smith, Janice Y.; Topping, Crystal C.; Britton, Sean M.; Bagby, Paul R.; Siochi, Emilie J.

2010-01-01

The motivation for this work is to develop self-healing polymeric materials to enable damage tolerant systems, and to tailor puncture healing for use temperatures and applications. This will be a benefit in environments and conditions where access for manual repair is limited or impossible, or where damage may not be detected.

7 CFR 993.97 - Exhibit A; minimum standards.

Code of Federal Regulations, 2010 CFR

2010-01-01

... condition; (3) end cracks; (4) fermentation; (5) skin or flesh damage; (6) scab; (7) burned; (8) mold; (9...) Fermentation means damage to the flesh by fermentation to the extent that the characteristic appearance or..., and decay shall not exceed five percent (5%). (3) The combined tolerance allowance for fermentation...

PrimPol prevents APOBEC/AID family mediated DNA mutagenesis

PubMed Central

Pilzecker, Bas; Buoninfante, Olimpia Alessandra; Pritchard, Colin; Blomberg, Olga S.; Huijbers, Ivo J.; van den Berk, Paul C.M.; Jacobs, Heinz

2016-01-01

Abstract PrimPol is a DNA damage tolerant polymerase displaying both translesion synthesis (TLS) and (re)-priming properties. This led us to study the consequences of a PrimPol deficiency in tolerating mutagenic lesions induced by members of the APOBEC/AID family of cytosine deaminases. Interestingly, during somatic hypermutation, PrimPol counteracts the generation of C>G transversions on the leading strand. Independently, mutation analyses in human invasive breast cancer confirmed a pro-mutagenic activity of APOBEC3B and revealed a genome-wide anti-mutagenic activity of PRIMPOL as well as most Y-family TLS polymerases. PRIMPOL especially prevents APOBEC3B targeted cytosine mutations within TpC dinucleotides. As C transversions induced by APOBEC/AID family members depend on the formation of AP-sites, we propose that PrimPol reprimes preferentially downstream of AP-sites on the leading strand, to prohibit error-prone TLS and simultaneously stimulate error-free homology directed repair. These in vivo studies are the first demonstrating a critical anti-mutagenic activity of PrimPol in genome maintenance. PMID:26926109

In vitro evaluation of the probiotic potential of Lactobacillus salivarius SMXD51.

PubMed

Messaoudi, S; Madi, A; Prévost, H; Feuilloley, M; Manai, M; Dousset, X; Connil, N

2012-12-01

Lactobacillus salivarius SMXD51 was previously isolated from the cecum of a Tunisian poultry and found to produce a bacteriocin-like substance highly active against the foodborne pathogen Campylobacter jejuni. The aim of this study was to examine some probiotic properties of the strain: acid and bile tolerance, capacity of adhesion, stimulation of immune defences (IL-6, IL-8, IL-10 and β-defensin 2), and modulation of the barrier integrity. The results showed that L. salivarius SMXD51 can tolerate gastrointestinal conditions (acid and bile), adhere to intestinal cells and stimulate the immune system. The bacterium strengthened the intestinal barrier functions through the increase of the transepithelial electrical resistance (TEER) and reinforcement of the F-actin cytoskeleton. One hour pretreatment with L. salivarius SMXD51 protected against Pseudomonas aeruginosa PAO1-induced decrease of TEER and damage of the F-actin cytoskeleton. Our results highlight that L. salivarius SMXD51 fulfils the principle requirements of an efficient probiotic and may be seen as a reliable candidate for further validation studies in chicken. Copyright © 2012 Elsevier Ltd. All rights reserved.

Composite transport wing technology development

NASA Technical Reports Server (NTRS)

Madan, Ram C.

1988-01-01

The design, fabrication, testing, and analysis of stiffened wing cover panels to assess damage tolerance criteria are discussed. The damage tolerance improvements were demonstrated in a test program using full-sized cover panel subcomponents. The panels utilized a hard skin concept with identical laminates of 44-percent 0-degree, 44-percent plus or minus 45-degree, and 12-percent 90-degree plies in the skins and stiffeners. The panel skins were impacted at midbay between the stiffeners, directly over the stiffener, and over the stiffener flange edge. The stiffener blades were impacted laterally. Impact energy levels of 100 ft-lb and 200 ft-lb were used. NASTRAN finite-element analyses were performed to simulate the nonvisible damage that was detected in the panels by nondestructive inspection. A closed-form solution for generalized loading was developed to evaluate the peel stresses in the bonded structure. Two-dimensional delamination growth analysis was developed using the principle of minimum potential energy in terms of closed-form solution for critical strain. An analysis was conducted to determine the residual compressive stress in the panels after impact damage, and the analytical predictions were verified by compression testing of the damaged panels.

Reduction of female copulatory damage by resilin represents evidence for tolerance in sexual conflict

PubMed Central

Michels, Jan; Gorb, Stanislav N.; Reinhardt, Klaus

2015-01-01

Intergenomic evolutionary conflicts increase biological diversity. In sexual conflict, female defence against males is generally assumed to be resistance, which, however, often leads to trait exaggeration but not diversification. Here, we address whether tolerance, a female defence mechanism known from interspecific conflicts, exists in sexual conflict. We examined the traumatic insemination of female bed bugs via cuticle penetration by males, a textbook example of sexual conflict. Confocal laser scanning microscopy revealed large proportions of the soft and elastic protein resilin in the cuticle of the spermalege, the female defence organ. Reduced tissue damage and haemolymph loss were identified as adaptive female benefits from resilin. These did not arise from resistance because microindentation showed that the penetration force necessary to breach the cuticle was significantly lower at the resilin-rich spermalege than at other cuticle sites. Furthermore, a male survival analysis indicated that the spermalege did not impose antagonistic selection on males. Our findings suggest that the specific spermalege material composition evolved to tolerate the traumatic cuticle penetration. They demonstrate the importance of tolerance in sexual conflict and genitalia evolution, extend fundamental coevolution and speciation models and contribute to explaining the evolution of complexity. We propose that tolerance can drive trait diversity. PMID:25673297

Reduction of female copulatory damage by resilin represents evidence for tolerance in sexual conflict.

PubMed

Michels, Jan; Gorb, Stanislav N; Reinhardt, Klaus

2015-03-06

Intergenomic evolutionary conflicts increase biological diversity. In sexual conflict, female defence against males is generally assumed to be resistance, which, however, often leads to trait exaggeration but not diversification. Here, we address whether tolerance, a female defence mechanism known from interspecific conflicts, exists in sexual conflict. We examined the traumatic insemination of female bed bugs via cuticle penetration by males, a textbook example of sexual conflict. Confocal laser scanning microscopy revealed large proportions of the soft and elastic protein resilin in the cuticle of the spermalege, the female defence organ. Reduced tissue damage and haemolymph loss were identified as adaptive female benefits from resilin. These did not arise from resistance because microindentation showed that the penetration force necessary to breach the cuticle was significantly lower at the resilin-rich spermalege than at other cuticle sites. Furthermore, a male survival analysis indicated that the spermalege did not impose antagonistic selection on males. Our findings suggest that the specific spermalege material composition evolved to tolerate the traumatic cuticle penetration. They demonstrate the importance of tolerance in sexual conflict and genitalia evolution, extend fundamental coevolution and speciation models and contribute to explaining the evolution of complexity. We propose that tolerance can drive trait diversity. © 2015 The Author(s) Published by the Royal Society. All rights reserved.

In field damage of high and low cyanogenic cassava due to a generalist insect herbivore Cyrtomenus bergi (Hemiptera: Cydnidae).

PubMed

Riis, Lisbeth; Bellotti, Anthony Charles; Castaño, Oscar

2003-12-01

The hypothesis that cyanogenic potential in cassava roots deters polyphagous insects in the field is relevant to current efforts to reduce or eliminate the cyanogenic potential in cassava. To test this hypothesis, experiments were conducted in the field under natural selection pressure of the polyphagous root feeder Cyrtomenus bergi Froeschner (Hemiptera: Cydnidae). A number of cassava varieties (33) as well as 13 cassava siblings and their parental clone, each representing a determined level of cyanogenic potential (CNP), were scored for damage caused by C. bergi and related to CNP and nonglycosidic cyanogens, measured as hydrogen cyanide. Additionally, 161 low-CNP varieties (< 50 ppm hydrogen cyanide, fresh weight) from the cassava germplasm core collection at Centro Internacional de Agricultura Tropical (CIAT) were screened for resistance/tolerance to C. bergi. Low root damage scores were registered at all levels of CNP. Nevertheless, CNP and yield (or root size) partly explained the damage in cassava siblings (r2 = 0.82) and different cassava varieties (r2 = 0.42), but only when mean values of damage scores were used. This relation was only significant in one of two crop cycles. A logistic model describes the underlying negative relation between CNP and damage. An exponential model describes the underlying negative relation between root size and damage. Damage, caused by C. bergi feeding, released nonglycosidic cyanogens, and an exponential model fits the underlying positive relation. Fifteen low-CNP clones were selected for potential resistance/tolerance against C. bergi.

Design and evaluation of a foam-filled hat-stiffened panel concept for aircraft primary structural applications

NASA Technical Reports Server (NTRS)

Ambur, Damodar R.

1995-01-01

A structurally efficient hat-stiffened panel concept that utilizes a structural foam as stiffener core has been designed for aircraft primary structural applications. This stiffener concept utilizes a manufacturing process that can be adapted readily to grid-stiffened structural configurations which possess inherent damage tolerance characteristics due to their multiplicity of load paths. The foam-filled hat-stiffener concept in a prismatically stiffened panel configuration is more efficient than most other stiffened panel configurations in a load range that is typical for both fuselage and wing structures. The prismatically stiffened panel concept investigated here has been designed using AS4/3502 preimpregnated tape and Rohacell foam core and evaluated for its buckling and postbuckling behavior with and without low-speed impact damage. The results from single-stiffener and multi-stiffener specimens suggest that this structural concept responds to loading as anticipated and has good damage tolerance characteristics.

Dehydration rate determines the degree of membrane damage and desiccation tolerance in bryophytes.

PubMed

Cruz de Carvalho, Ricardo; Catalá, Myriam; Branquinho, Cristina; Marques da Silva, Jorge; Barreno, Eva

2017-03-01

Desiccation tolerant (DT) organisms are able to withstand an extended loss of body water and rapidly resume metabolism upon rehydration. This ability, however, is strongly dependent on a slow dehydration rate. Fast dehydration affects membrane integrity leading to intracellular solute leakage upon rehydration and thereby impairs metabolism recovery. We test the hypothesis that the increased cell membrane damage and membrane permeability observed under fast dehydration, compared with slow dehydration, is related to an increase in lipid peroxidation. Our results reject this hypothesis because following rehydration lipid peroxidation remains unaltered, a fact that could be due to the high increase of NO upon rehydration. However, in fast-dried samples we found a strong signal of red autofluorescence upon rehydration, which correlates with an increase in ROS production and with membrane leakage, particularly the case of phenolics. This could be used as a bioindicator of oxidative stress and membrane damage. © 2016 Scandinavian Plant Physiology Society.

High Performance COPVs for In-Space Storage of High Pressure Cryogenic Fuels

NASA Technical Reports Server (NTRS)

Schneider, Judy; Dyess, Mark; Hastings, Chad; Wang, Jun

2008-01-01

Polymeric composite overwrapped pressure vessels (COPVs) provide an attractive material system to support developing commercial launch business and alternate fuel ventures. However to be able to design with these materials, the mechanical behavior of the materials must be understood with regards to processing, performance, damage tolerance, and environment. For the storage of cryogenic propellants, it is important to evaluate the materials performance and impact damage resistance at cryogenic temperatures in order to minimize weight and to ensure safety and reliability. As part of this study, material tests of candidate fiber and resin systems were used as the basis for the selection of the material combinations for evaluation in a COPV at cryogenic conditions. This comprehensive approach has also been expanded to address issues with impact damage tolerance and material degradation due to environmental factors. KEY WORDS: Cryogenic testing, evaluation and applications for pressure vessels, COPVs, tanks, or storage vessels.

Value contamination avoidance devices

NASA Technical Reports Server (NTRS)

Endicott, D. L.

1975-01-01

Mechanical redesign methods were used to minimize contamination damage of conventional fluid components and a contamination separator device was developed for long term reusable space vehicles. These were incorporated into an existing 50.8 mm poppet valve and tested for damage tolerance in a full size open loop flow system with gaseous and liquid nitrogen. Cyclic and steady flow conditions were tested with particles of 125 to 420 micrometers aluminum oxide dispersed in the test fluids. Nonflow life tests (100,000 cycles) were made with two valve configurations in gaseous hydrogen. The redesigned valve had an acceptable cycle life and improved tolerance to contamination damage when the primary sealing surfaces were coated with thin coatings of hard plastic (Teflon S and Kynar). Analytical studies and flow testing were completed of four different versions of the separator. overall separation efficiencies in the 55-90% range were measured with these non-optimum configurations.

Effect of Buckling Modes on the Fatigue Life and Damage Tolerance of Stiffened Structures

NASA Technical Reports Server (NTRS)

Davila, Carlos G.; Bisagni, Chiara; Rose, Cheryl A.

2015-01-01

The postbuckling response and the collapse of composite specimens with a co-cured hat stringer are investigated experimentally and numerically. These specimens are designed to evaluate the postbuckling response and the effect of an embedded defect on the collapse load and the mode of failure. Tests performed using controlled conditions and detailed instrumentation demonstrate that the damage tolerance, fatigue life, and collapse loads are closely tied with the mode of the postbuckling deformation, which can be different between two nominally identical specimens. Modes that tend to open skin/stringer defects are the most damaging to the structure. However, skin/stringer bond defects can also propagate under shearing modes. In the proposed paper, the effects of initial shape imperfections on the postbuckling modes and the interaction between different postbuckling deformations and the propagation of skin/stringer bond defects under quasi-static or fatigue loads will be examined.

Response of antioxidant system to drought stress and re-watering in Alfalfa during branching

NASA Astrophysics Data System (ADS)

Tina, R. R.; Shan, X. R.; Wang, Y.; Guo, S. Y.; Mao, B.; Wang, W.; Wu, H. Y.; Zhao, T. H.

2017-11-01

This paper aimed to reveal the response mechanism of active oxygen metabolism and antioxidant enzyme activities in Alfalfa under drought stress and re-watering, and the pot experiment was used, to explore the changes of H2O2, O2·-, electrolyte leakage conductivity and MDA, SOD, POD, CAT activity in Golden Empress (tolerant cultivar) and Sanditi (non-tolerant cultivar) under drought stress and re-watering during branching stage. Three water gradients were set up: CK (Maximum field capacity of 75%±5%), T1 (Maximum field capacity of 45%±5%), T2 (Maximum field capacity of 35%±5%) to compare, and the drought rehydration was also studied. Results: the results indicated that H2O2 content, O2·-production rate, relative conductivity and MDA content were higher than the control, and the increase extent of Golden Empress was higher than the Sanditi under drought stress and after re-watering the recovery capability of Golden Empress was also higher than the Sanditi. After 7 days of re-watering, all indexes were restored to the control level, indicating that the re-watering have compensation effect after drought. After drought stress, to weaken the damage of active oxygen Golden Empress was mainly by increasing the activity of POD and SOD, but Sanditi was mainly through the POD and CAT activity increased to effectively remove ROS. Under drought stress, active oxygen in leaves of Alfalfa increased, and thus the membrane system was damaged which lead to the increase of MDA content and relative electric conductivity. Plants play a defensive role by increasing the activity of antioxidant enzymes and scavenging reactive oxygen species. After re-watering, the stress effect was reduced, and the physiological indexes of plants were restored to the control level. In general, tolerant cultivar has stronger antioxidant properties under drought and re-watering.

Processing and mechanical characterization of alumina laminates

NASA Astrophysics Data System (ADS)

Montgomery, John K.

2002-08-01

Single-phase ceramics that combine property gradients or steps in monolithic bodies are sought as alternatives to ceramic composites made of dissimilar materials. This work describes novel processing methods to produce stepped-density (or laminated) alumina single-phase bodies that maintain their mechanical integrity. One arrangement consists of a stiff, dense bulk material with a thin, flaw tolerant, porous exterior layer. Another configuration consists of a lightweight, low-density bulk material with a thin, hard, wear resistant exterior layer. Alumina laminates with strong interfaces have been successfully produced in this work using two different direct-casting processes. Gelcasting is a useful near-net shape processing technique that has been combined with several techniques, such as reaction bonding of aluminum oxide and the use of starch as a fugative filler, to successfully produced stepped-density alumina laminates. The other direct casting process that has been developed in this work is thermoreversible gelcasting (TRG). This is a reversible gelation process that has been used to produce near-net shape dense ceramic bodies. Also, individual layers can be stacked together and heated to produce laminates. Bilayer laminate samples were produced with varied thickness of porous and dense layers. It was shown that due to the difference in modulus and hardness, transverse cracking is found upon Hertzian contact when the dense layer is on the exterior. In the opposite arrangement, compacted damage zones formed in the porous material and no damage occurred in the underlying dense layer. Flaw tolerant behavior of the porous exterior/dense underlayer was examined by measuring biaxial strength as a function of Vickers indentation load. It was found that the thinnest layer of porous material results in the greatest flaw tolerance. Also, higher strength was exhibited at large indentation loads when compared to dense monoliths. The calculated stresses on the surfaces and interface afforded an explanation of the behavior that failure initiates at the interface between the layers for the thinnest configuration, rather than the sample surface.

High density array screening to identify the genetic requirements for transition metal tolerance in Saccharomyces cerevisiae.

PubMed

Bleackley, Mark R; Young, Barry P; Loewen, Christopher J R; MacGillivray, Ross T A

2011-02-01

Biological systems have developed with a strong dependence on transition metals for accomplishing a number of biochemical reactions. Iron, copper, manganese and zinc are essential for virtually all forms of life with their unique chemistries contributing to a variety of physiological processes including oxygen transport, generation of cellular energy and protein structure and function. Properties of these metals (and to a lesser extent nickel and cobalt) that make them so essential to life also make them extremely cytotoxic in many cases through the formation of damaging oxygen radicals via Fenton chemistry. While life has evolved to exploit the chemistries of transition metals to drive physiological reactions, systems have concomitantly evolved to protect against the damaging effects of these same metals. Saccharomyces cerevisiae is a valuable tool for studying metal homeostasis with many of the genes identified thus far having homologs in higher eukaryotes including humans. Using high density arrays, we have screened a haploid S. cerevisiae deletion set containing 4786 non-essential gene deletions for strains sensitive to each of Fe, Cu, Mn, Ni, Zn and Co and then integrated the six screens using cluster analysis to identify pathways that are unique to individual metals and others with function shared between metals. Genes with no previous implication in metal homeostasis were found to contribute to sensitivity to each metal. Significant overlap was observed between the strains that were sensitive to Mn, Ni, Zn and Co with many of these strains lacking genes for the high affinity Fe transport pathway and genes involved in vacuolar transport and acidification. The results from six genome-wide metal tolerance screens show that there is some commonality between the cellular defenses against the toxicity of Mn, Ni, Zn and Co with Fe and Cu requiring different systems. Additionally, potential new factors been identified that function in tolerance to each of the six metals.

Distributed sensing of Composite Over-wrapped Pressure Vessels using Fiber-Bragg Gratings

NASA Technical Reports Server (NTRS)

Grant, Joseph

2005-01-01

The increasing use of advanced composite materials in the wide range of applications including Space Structures is a great impetus to the development of smart materials. These materials offer a wide range of possibilities within the space program. But before they can be reliably incorporated into space flight applications, additional understanding is required in the area of damage tolerance of these materials. Efforts to enhance our understanding of failure modes, mechanical properties, long and short term environmental effects, cyclic damage accumulation and residual strength are needed. Thus we have employed the use of fiber optical sensors which offers an excellent opportunity exploit these materials through monitoring and characterizing their mechanical properties and thus the integrity of structures made from such materials during their life cycle. Use of these optical innovations provides an insight into structures that have not been available in the past, as well as the technology available to provide real time health monitoring throughout its life cycle. The embedded fiber optical sensor shows a clearly detectable sensitivity to changes in the near strain and stress fields of the host structure promoted by mechanical or thermal loading or, in certain conditions, structural damage. The last ten years have seen a large increase in the use of FBG based monitoring systems in a broad range of applications. Fiber Bragg gratings are use to monitor the structural properties of composite pressure vessels. These gratings optically inscribed into the core of a single mode fiber are used as a tool to monitor the stress strain relation in composite structures. The fiber Bragg sensors are both embedded within the composite laminates and bonded to the surface of the vessel with varying orientations with respect to the carbon fiber in the epoxy matrix. The response of these fiber-optic sensors is investigated by pressurizing the cylinder up to its burst pressure of around 4400 psi. This is done at both ambient and cryogenic temperatures using water and liquid nitrogen. The recorded response is compared with the response from conventional strain gauge also present on the vessel. Additionally, several vessels were tested that had been damaged to simulate different type of events, such as cut tow, delimitation and impact damage.

Improvement of the functional properties of nanostructured Ti-Ni shape memory alloys by means of thermomechanical processing

NASA Astrophysics Data System (ADS)

Kreitcberg, Alena

Severe plastic deformation (SPD) is commonly used for nanostructure formation in Ti-Ni shape memory alloys (SMAs), but it increases the risk of damage during processing and, consequently, negatively affects functional fatigue resistance of these materials. The principal objective of this project is, therefore, to study the interrelations between the processing conditions, damageability during processing, microstructure and the functional properties of Ti-Ni SMAs with the aim of improving long-term functional performances of these materials by optimizing their processing conditions. First, microstructure and fatigue properties of Ti-Ni SMAs were studied after thermomechanical treatment (TMT) with different combinations of severe cold and warm rolling (CR and WR), as well as intermediate and post-deformation annealing (IA and PDA) technological steps. It was shown that either when WR and IA were introduced into the TMT schedule, or CR intensity was decreased, the fatigue life was improved as a consequence of less processing-induced damage and higher density of the favorable B2-austenite texture. This improvement was reached, however, at a price of a lower multi-cycle functional stability of these materials, the latter being a direct consequence of the microstructure coarsening after higher-temperature lower-intensity processing. At the end of this study, however, it was not possible to distinguish between contributions to the functional performances of Ti-Ni SMAs from different processing-related features: a) grain/subgrain size; b) texture; and c) level of rolling-induced defects. To be capable of separating contributions to the functional properties of Ti-Ni alloys from grain/subgrain size and from texture, the theoretical crystallographic resource of recovery strain after different TMTs and, therefore, different textures, were calculated and compared with the experiment. The comparative analysis showed that the structural factors (grain/subgrain size) strongly dominate the texture contributions, and therefore, there is no real alternative to having nanocrystalline Ti-Ni alloys, if one needs to maximize the Ti-Ni alloys functional properties. Since the creation of such a microstructure requires the use of severe cold deformation techniques and neither of these techniques can be completely exempt from defects, it was deemed necessary to compare the damage tolerance of nanocrystalline Ti-Ni alloys to that of their nanosubgrained and mixed nanocrystalline/nanosubgrained counterparts. With this objective in mind, a detailed analysis of interrelations between the level of the CR/WR-induced damage (edge microcrack size and concentration) and the fatigue life of Ti- Ni SMAs was carried out. It was shown that nanocrystalline structure provides higher tolerance to small-crack propagation than nanosubgrained or mixed nanocrystalline/ nanosubgrained structures, and that low-temperature deformability of these alloys has to be improved to benefit from the property-enhancement potential of nanocrystalline structure. To broaden our knowledge in the field of Ti-Ni alloy deformability, the strain-rate sensitivity of these alloys was studied. Different microstructures, varying from the coarse- to ultrafinegrained, were created by means of equal-channel angular pressing (ECAP) and subjected to strain-rate sensitivity testing. As a result, the material with ultrafine-grained microstructure demonstrated an improved deformability as compared to the coarse-grained structure, at any deformation temperature. Moreover, it was determined that the smaller the grain size, the lower the temperature and the higher the strain-rate at which superplasticity occurs. Based on the results obtained, combined thermomechanical processing (ECAP at elevated temperatures followed by CR) was proposed and validated in terms of structural refinement with reduced level of processing-induced defects. Scientific contributions. This thesis contributes to the advancement of knowledge in the field of Ti-Ni SMAs' processing-structure-properties interactions, and the main conclusions of this study can be summed-up as follows: • Nanocrystalline Ti-Ni alloys significantly outperform nanosubgrain Ti-Ni alloys in terms of the absolute values and stability of their single- and multiple-cycle functional properties (superelasticity and shape memory characteristics). The main factor limiting the number of cycles to failure of the nanocrystalline alloys is the processingrelated damage. • The structure of Ti-Ni alloys plays significantly higher role in the realization of their functional potential that does their texture. • In terms of fatigue life, the nanocrystalline structure has lower small-crack sensitivity than does the nanosubgrained structure. • Grain refinement makes it possible to improve deformability of Ti-Ni alloys at any temperature. • To produce nanocrystalline Ti-Ni SMAs free of processing-induced-defects, a novel three-step processing is proposed (ECAP+CR+PDA): grain-refining severe plastic deformation at elevated temperatures (ECAP), followed-up by amorphizing SPD at low temperatures (CR), and ended-up by nanocrystallizing post-deformation heat treatment (PDA).

Rad18 confers hematopoietic progenitor cell DNA damage tolerance independently of the Fanconi Anemia pathway in vivo

PubMed Central

Yang, Yang; Poe, Jonathan C.; Yang, Lisong; Fedoriw, Andrew; Desai, Siddhi; Magnuson, Terry; Li, Zhiguo; Fedoriw, Yuri; Araki, Kimi; Gao, Yanzhe; Tateishi, Satoshi; Sarantopoulos, Stefanie; Vaziri, Cyrus

2016-01-01

In cultured cancer cells the E3 ubiquitin ligase Rad18 activates Trans-Lesion Synthesis (TLS) and the Fanconi Anemia (FA) pathway. However, physiological roles of Rad18 in DNA damage tolerance and carcinogenesis are unknown and were investigated here. Primary hematopoietic stem and progenitor cells (HSPC) co-expressed RAD18 and FANCD2 proteins, potentially consistent with a role for Rad18 in FA pathway function during hematopoiesis. However, hematopoietic defects typically associated with fanc-deficiency (decreased HSPC numbers, reduced engraftment potential of HSPC, and Mitomycin C (MMC) -sensitive hematopoiesis), were absent in Rad18−/− mice. Moreover, primary Rad18−/− mouse embryonic fibroblasts (MEF) retained robust Fancd2 mono-ubiquitination following MMC treatment. Therefore, Rad18 is dispensable for FA pathway activation in untransformed cells and the Rad18 and FA pathways are separable in hematopoietic cells. In contrast with responses to crosslinking agents, Rad18−/− HSPC were sensitive to in vivo treatment with the myelosuppressive agent 7,12 Dimethylbenz[a]anthracene (DMBA). Rad18-deficient fibroblasts aberrantly accumulated DNA damage markers after DMBA treatment. Moreover, in vivo DMBA treatment led to increased incidence of B cell malignancy in Rad18−/− mice. These results identify novel hematopoietic functions for Rad18 and provide the first demonstration that Rad18 confers DNA damage tolerance and tumor-suppression in a physiological setting. PMID:26883629

Proceedings of the Annual Mechanics of Composites Review (6th),

DTIC Science & Technology

1981-02-01

containing and resisting damage in compression-loaded composite structural components. 176( CONTRACTS INCREMENTAL ANALYSIS OF IMPACT DAMAGE NASI -15888 79...EVALUATION OF THE DURABILITY AND DAMAGE TOLERANCE OF CTOL COMPOS- ITE STRUCTURES NASI -15107 77 October 12 - 80 October 11 Project Engineer: Edward P...DEVELOPMENT OF AN ORTHOTROPIC HOLE ELEMENT NASI -15890 79 July 9 - 80 September 24 Project Engineer: Dr. John H. Crews, Jr. Mail Stop 18BE NASA Langley

Genetic compensation of high dose radiation-induced damage in an anhydrobiotic insect

NASA Astrophysics Data System (ADS)

Gusev, Oleg; Nakahara, Yuichi; Sakashita, Tetsuya; Kikawada, Takahiro; Okuda, Takashi

Anhydrobiotic larvae of African chironomid Polypedilum vanderplanki are known to show an extremely high tolerance against a range of stresses. The tolerance against various extreme environments exhibited by that insect might be due to being almost completely desiccated replacing water with trehalose, a state where little or no chemical reactions occur. From 2005 dried larvae of this insect are being used in a number of space experiments, both inside and outside of ISS as a model organism for estimation the limits of higher organisms' resistance to space environment stresses and long-term storage of the alive anhydrobiotic organisms during continues spaceflight. We have shown previously that both hydrated and dried larvae of Polypedilum vanderplanki have very higher tolerance against both highand low-linear energy transfer (LET), surviving after 7000Gy irradiation. It was suggested that the larvae would have effective DNA-reparation system in addition to artificial protection provided by glass-stage without water. In the present study we conducted analysis of stress-related gene expression in the larvae after 70-2000 Gy irradiations. Both DNA damage level and activity of DNA-reparation, anti-apoptotic and protein-damage related genes were analyzed. Direct visualization of DNA damage in the larvae fat body cells using Comet Assay showed that fragmented by radiation DNA is re-arranged within 76-98 hours after exposure. We found that massive overexpression of hsp and anti-oxidant genes occur in larvae entering anhydrobiosis , and provides refolding of proteins after rehydration. In the irradiated larvae overexpression of DNA-reparation enzymes anti-apoptotic genes was confirmed, suggesting that survival after high-dose irradiation is a result of combination of highly effective blocking of entering the apoptosis after severe DNA damage and DNA reparation.

Aluminum-lithium alloys in helicopters

DOE Office of Scientific and Technical Information (OSTI.GOV)

Smith, A.F.

1997-10-01

Aluminium-lithium alloys are widely applied on the EH101 helicopter, designed and built jointly by GKN Westland Helicopters of England and Agusta S.p.A. of Italy. With the exception of the powder metallurgy alloy AA 5091, all the current commercially available aluminum-lithium alloys are produced by direct-chill casting, and require a precipitation-aging heat treatment to achieve the required properties. In aluminum-lithium alloys containing greater than 1.3% (by weight) of lithium, the intermetallic phase {delta}{prime}-Al{sub 3}Li precipitates upon natural or artificial aging, but the associated strengthening effect is insufficient to meet the medium or high strength levels usually required (the damage tolerant tempermore » in AA 8090 is an exception).« less

Delamination properity of 2060 aluminium lithium alloy laminate

NASA Astrophysics Data System (ADS)

Guo, Xiang; Huang, Yi; Tong, Dihua; Ma, Shaojun

2018-03-01

Delamination is an significant property of aluminium lithium alloy laminate for damage tolerance design. Double Crack Lap Shear specimens of "2/1" structure are used for testing delamination properity. Experiments of 2 stress ratio conditions R=0.06, 0.5 are conducted, and 5 stress levels for each stress ratio. Delamination growth data b-N are obtained from 4 crack tips’ locations. The energy release rate, Gd, of the "2/1" structure laminate are calculated. Alderliesten modelis used for describing delamination growth db/dN and energy release rate Gd. Parameters Cd and nd in Alderliesten model are determined for R=0.06 and R=0.5 by linear fitting. An analysis is made by comparing the results of different stress ratio conditons.

Tough, processable simultaneous semi-interpenetrating polyimides

NASA Technical Reports Server (NTRS)

Pater, Ruth H. (Inventor)

1994-01-01

A high temperature semi-interpenetrating polymer network (semi-IPN) was developed which had significantly improved processability, damage tolerance, and mechanical performance when compared to the commercial Thermid materials. This simultaneous semi-IPN was prepared by mixing a thermosetting polyimide with a thermoplastic monomer precursor solution (NR150B2) and allowing them to react upon heating. This reaction occurs at a rate which decreases the flow and broadens the processing window. Upon heating at a higher temperature, there is an increase in flow. Because of the improved flow properties, broadened processing window and enhanced toughness, high strength polymer matrix composites, adhesives and molded articles can now be prepared from the acetylene endcapped polyimides which were previously inherently brittle and difficult to process.

A novel biomimetic approach to the design of high-performance ceramic–metal composites

PubMed Central

Launey, Maximilien E.; Munch, Etienne; Alsem, Daan Hein; Saiz, Eduardo; Tomsia, Antoni P.; Ritchie, Robert O.

2010-01-01

The prospect of extending natural biological design to develop new synthetic ceramic–metal composite materials is examined. Using ice-templating of ceramic suspensions and subsequent metal infiltration, we demonstrate that the concept of ordered hierarchical design can be applied to create fine-scale laminated ceramic–metal (bulk) composites that are inexpensive, lightweight and display exceptional damage-tolerance properties. Specifically, Al2O3/Al–Si laminates with ceramic contents up to approximately 40 vol% and with lamellae thicknesses down to 10 µm were processed and characterized. These structures achieve an excellent fracture toughness of 40 MPa√m at a tensile strength of approximately 300 MPa. Salient toughening mechanisms are described together with further toughening strategies. PMID:19828498

Adaptations of quaking aspen for defense against damage by herbivores and related environmental agents

Treesearch

Richard L. Lindroth

2001-01-01

Quaking aspen (Populus tremuloides) employs two major systems of defense against damage by environmental agents: chemical defense and tolerance. Aspen accumulates appreciable quantities of phenolic glycosides (salicylates) and condensed tannins in most tissues and accumulates coniferyl benzoate in flower buds. Phenolic glycosides are toxic and/or deterrent to pathogens...

Pharmacodynamic Assay Panel for Monitoring Phospho-Signaling Networks | Office of Cancer Clinical Proteomics Research

Cancer.gov

The DNA damage response (DDR) is a highly regulated signal transduction network that orchestrates the temporal and spatial organization of protein complexes required to repair (or tolerate) DNA damage (e.g., nucleotide excision repair, base excision repair, homologous recombination, non-homologous end joining, post-replication repair).

Reversing Optical Damage In LiNbO3 Switches

NASA Technical Reports Server (NTRS)

Gee, C. M.; Thurmond, G. D.

1985-01-01

One symptom of optical damage in Ti-diffused LiNbO3 directional-coupler switch reversed by temporarily raising input illumination to higher-thannormal power level. Healing phenomenon used to restore normal operation, increase operating-power rating, and stabilize operating characteristics at lower powers. Higher operating power is tolerated after treatment.

Cell injury and repair resulting from sleep loss and sleep recovery in laboratory rats.

PubMed

Everson, Carol A; Henchen, Christopher J; Szabo, Aniko; Hogg, Neil

2014-12-01

Increased cell injury would provide the type of change in constitution that would underlie sleep disruption as a risk factor for multiple diseases. The current study was undertaken to investigate cell injury and altered cell fate as consequences of sleep deprivation, which were predicted from systemic clues. Partial (35% sleep reduction) and total sleep deprivation were produced in rats for 10 days, which was tolerated and without overtly deteriorated health. Recovery rats were similarly sleep deprived for 10 days, then allowed undisturbed sleep for 2 days. The plasma, liver, lung, intestine, heart, and spleen were analyzed and compared to control values for damage to DNA, proteins, and lipids; apoptotic cell signaling and death; cell proliferation; and concentrations of glutathione peroxidase and catalase. Oxidative DNA damage in totally sleep deprived rats was 139% of control values, with organ-specific effects in the liver (247%), lung (166%), and small intestine (145%). Overall and organ-specific DNA damage was also increased in partially sleep deprived rats. In the intestinal epithelium, total sleep deprivation resulted in 5.3-fold increases in dying cells and 1.5-fold increases in proliferating cells, compared with control. Recovery sleep restored the balance between DNA damage and repair, and resulted in normal or below-normal metabolic burdens and oxidative damage. These findings provide physical evidence that sleep loss causes cell damage, and in a manner expected to predispose to replication errors and metabolic abnormalities; thereby providing linkage between sleep loss and disease risk observed in epidemiological findings. Properties of recovery sleep include biochemical and molecular events that restore balance and decrease cell injury. © 2014 Associated Professional Sleep Societies, LLC.

The stomatopod dactyl club: a formidable damage-tolerant biological hammer.

PubMed

Weaver, James C; Milliron, Garrett W; Miserez, Ali; Evans-Lutterodt, Kenneth; Herrera, Steven; Gallana, Isaias; Mershon, William J; Swanson, Brook; Zavattieri, Pablo; DiMasi, Elaine; Kisailus, David

2012-06-08

Nature has evolved efficient strategies to synthesize complex mineralized structures that exhibit exceptional damage tolerance. One such example is found in the hypermineralized hammer-like dactyl clubs of the stomatopods, a group of highly aggressive marine crustaceans. The dactyl clubs from one species, Odontodactylus scyllarus, exhibit an impressive set of characteristics adapted for surviving high-velocity impacts on the heavily mineralized prey on which they feed. Consisting of a multiphase composite of oriented crystalline hydroxyapatite and amorphous calcium phosphate and carbonate, in conjunction with a highly expanded helicoidal organization of the fibrillar chitinous organic matrix, these structures display several effective lines of defense against catastrophic failure during repetitive high-energy loading events.

The Stomatopod Dactyl Club: A Formidable Damage-Tolerant Biological Hammer

DOE Office of Scientific and Technical Information (OSTI.GOV)

Weaver J. C.; DiMasi E.; Milliron, G.W.

2012-06-08

Nature has evolved efficient strategies to synthesize complex mineralized structures that exhibit exceptional damage tolerance. One such example is found in the hypermineralized hammer-like dactyl clubs of the stomatopods, a group of highly aggressive marine crustaceans. The dactyl clubs from one species, Odontodactylus scyllarus, exhibit an impressive set of characteristics adapted for surviving high-velocity impacts on the heavily mineralized prey on which they feed. Consisting of a multiphase composite of oriented crystalline hydroxyapatite and amorphous calcium phosphate and carbonate, in conjunction with a highly expanded helicoidal organization of the fibrillar chitinous organic matrix, these structures display several effective lines ofmore » defense against catastrophic failure during repetitive high-energy loading events.« less

Nanolattices: An Emerging Class of Mechanical Metamaterials.

PubMed

Bauer, Jens; Meza, Lucas R; Schaedler, Tobias A; Schwaiger, Ruth; Zheng, Xiaoyu; Valdevit, Lorenzo

2017-10-01

In 1903, Alexander Graham Bell developed a design principle to generate lightweight, mechanically robust lattice structures based on triangular cells; this has since found broad application in lightweight design. Over one hundred years later, the same principle is being used in the fabrication of nanolattice materials, namely lattice structures composed of nanoscale constituents. Taking advantage of the size-dependent properties typical of nanoparticles, nanowires, and thin films, nanolattices redefine the limits of the accessible material-property space throughout different disciplines. Herein, the exceptional mechanical performance of nanolattices, including their ultrahigh strength, damage tolerance, and stiffness, are reviewed, and their potential for multifunctional applications beyond mechanics is examined. The efficient integration of architecture and size-affected properties is key to further develop nanolattices. The introduction of a hierarchical architecture is an effective tool in enhancing mechanical properties, and the eventual goal of nanolattice design may be to replicate the intricate hierarchies and functionalities observed in biological materials. Additive manufacturing and self-assembly techniques enable lattice design at the nanoscale; the scaling-up of nanolattice fabrication is currently the major challenge to their widespread use in technological applications. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Local-based damage detection of cyclically loaded bridge piers using wireless sensing units

NASA Astrophysics Data System (ADS)

Hou, Tsung-Chin; Lynch, Jerome P.; Parra-Montesinos, Gustavo

2005-05-01

Concrete bridge piers are a common structural element employed in the design of bridges and elevated roadways. In order to ensure adequate behavior under earthquake-induced displacements, extensive reinforcement detailing in the form of closely spaced ties or spirals is necessary, leading to congestion problems and difficulties during concrete casting. Further, costly repairs are often necessary in bridge piers after a major earthquake which in some cases involve the total or partial shutdown of the bridge. In order to increase the damage tolerance while relaxing the transverse reinforcement requirements of bridge piers, the use of high-performance fiber reinforced cementitious composites (HPFRCC) in earthquake-resistant bridge piers is explored. HPFRCCs are a relatively new class of cementitious material for civil structures with tensile strain-hardening behavior and high damage tolerance. To monitor the behavior of this new class of material in the field, low-cost wireless monitoring technologies will be adopted to provide HPFRCC structural elements the capability to accurately monitor their performance and health. In particular, the computational core of a wireless sensing unit can be harnessed to screen HPFRCC components for damage in real-time. A seismic damage index initially proposed for flexure dominated reinforced concrete elements is modified to serve as an algorithmic tool for the rapid assessment of damage (due to flexure and shear) in HPFRCC bridge piers subjected to large shear reversals. Traditional and non-traditional sensor strategies of an HPFRCC bridge pier are proposed to optimize the correlation between the proposed damage index model and the damage observed in a circular pier test specimen. Damage index models are shown to be a sufficiently accurate rough measure of the degree of local-area damage that can then be wirelessly communicated to bridge officials.

29 CFR 15.205 - What types of claims for property damage are allowed under the MPCECA?

Code of Federal Regulations, 2013 CFR

2013-07-01

... 29 Labor 1 2013-07-01 2013-07-01 false What types of claims for property damage are allowed under... Civilian Employees' Claims Act of 1964 § 15.205 What types of claims for property damage are allowed under the MPCECA? (a) Claims for property damage are allowed under the MPCECA only if the property involved...

29 CFR 15.205 - What types of claims for property damage are allowed under the MPCECA?

Code of Federal Regulations, 2014 CFR

2014-07-01

... 29 Labor 1 2014-07-01 2013-07-01 true What types of claims for property damage are allowed under... Civilian Employees' Claims Act of 1964 § 15.205 What types of claims for property damage are allowed under the MPCECA? (a) Claims for property damage are allowed under the MPCECA only if the property involved...

Hydraulics and life history of tropical dry forest tree species: coordination of species' drought and shade tolerance.

PubMed

Markesteijn, Lars; Poorter, Lourens; Bongers, Frans; Paz, Horacio; Sack, Lawren

2011-07-01

Plant hydraulic architecture has been studied extensively, yet we know little about how hydraulic properties relate to species' life history strategies, such as drought and shade tolerance. The prevailing theories seem contradictory. We measured the sapwood (K(s) ) and leaf (K(l) ) hydraulic conductivities of 40 coexisting tree species in a Bolivian dry forest, and examined associations with functional stem and leaf traits and indices of species' drought (dry-season leaf water potential) and shade (juvenile crown exposure) tolerance. Hydraulic properties varied across species and between life-history groups (pioneers vs shade-tolerant, and deciduous vs evergreen species). In addition to the expected negative correlation of K(l) with drought tolerance, we found a strong, negative correlation between K(l) and species' shade tolerance. Across species, K(s) and K(l) were negatively correlated with wood density and positively with maximum vessel length. Consequently, drought and shade tolerance scaled similarly with hydraulic properties, wood density and leaf dry matter content. We found that deciduous species also had traits conferring efficient water transport relative to evergreen species. Hydraulic properties varied across species, corresponding to the classical trade-off between hydraulic efficiency and safety, which for these dry forest trees resulted in coordinated drought and shade tolerance across species rather than the frequently hypothesized trade-off. © 2011 The Authors. New Phytologist © 2011 New Phytologist Trust.

Some Biological Hints on the Control of Heat and Mass Transfer

NASA Astrophysics Data System (ADS)

Hagiwara, Yoshimichi

This review paper explores the possibilities of the control of heat and mass transfer associated with drought tolerance and freeze tolerance. The accumulation of some metabolites, such as proline and trehalose, are effective for drought tolerance. The special microstructures on the surfaces of some plants and insects in deserts are effective for collecting moisture in the air. Methods of preserving crops will be improved by the mimetic of the drought tolerance. Calcium ions and a protein are effective for the retrieval of damaged cell membrane due to ice formation. Ice crystal growth is inhibited by some substances such as antifreeze proteins. The cryopreservation of foods and organs will be improved by the mimetic of the freeze tolerance.

Lightning Strike Induced Damage Mechanisms of Carbon Fiber Composites

NASA Astrophysics Data System (ADS)

Kawakami, Hirohide

Composite materials have a wide application in aerospace, automotive, and other transportation industries, because of the superior structural and weight performances. Since carbon fiber reinforced polymer composites possess a much lower electrical conductivity as compared to traditional metallic materials utilized for aircraft structures, serious concern about damage resistance/tolerance against lightning has been rising. Main task of this study is to clarify the lightning damage mechanism of carbon fiber reinforced epoxy polymer composites to help further development of lightning strike protection. The research on lightning damage to carbon fiber reinforced polymer composites is quite challenging, and there has been little study available until now. In order to tackle this issue, building block approach was employed. The research was started with the development of supporting technologies such as a current impulse generator to simulate a lightning strike in a laboratory. Then, fundamental electrical properties and fracture behavior of CFRPs exposed to high and low level current impulse were investigated using simple coupon specimens, followed by extensive parametric investigations in terms of different prepreg materials frequently used in aerospace industry, various stacking sequences, different lightning intensity, and lightning current waveforms. It revealed that the thermal resistance capability of polymer matrix was one of the most influential parameters on lightning damage resistance of CFRPs. Based on the experimental findings, the semi-empirical analysis model for predicting the extent of lightning damage was established. The model was fitted through experimental data to determine empirical parameters and, then, showed a good capability to provide reliable predictions for other test conditions and materials. Finally, structural element level lightning tests were performed to explore more practical situations. Specifically, filled-hole CFRP plates and patch-repaired CFRP plates were selected as structural elements likely to be susceptible to lightning event. This study forms a solid foundation for the understanding of lightning damage mechanism of CFRPs, and become an important first step toward building a practical damage prediction tool of lighting event.

The paradox of higher light tolerance during desiccation in rare old forest cyanolichens than in more widespread co-occurring chloro- and cephalolichens

PubMed Central

Gauslaa, Yngvar; Coxson, Darwyn S; Solhaug, Knut Asbjørn

2012-01-01

Desiccation tolerance was quantified in four cyanolichens (Lobaria hallii, Lobaria retigera, Lobaria scrobiculata, Pseudocyphellaria anomala), one cephalolichen (Lobaria pulmonaria) and one chlorolichen (Platismatia glauca) from xeric and mesic, open and closed North American boreal forests. These sympatric epiphytes were exposed to 0%, 33%, 55% and 75% relative humidity with or without medium light (200 μmol m−2 s−1) for 7 d. Permanent and temporary photoinhibitory damage was recorded as viability measures. All species tolerated well the drying in darkness, but L. hallii and L. retigera, associated with a very humid climate, showed minor damage at the hardest drying (silica gel). Simultaneous exposure to medium light severely aggravated the drying damage at all relative humidity levels. Combined drying–light exposure was particularly devastating for the widespread chloro- and cephalolichens, whereas cyanolichens, including rare old forest species, were fairly resistant. The ability to recover after combined drying–light stress (this study) correlated positively with increasing species-specific water holding capacities (from the literature). Cyanolichens, depending on liquid water and large internal water storage, probably require strong drying–light resistance to handle long periods between hydration events, whereas chlorolichens can regularly maintain their photosynthetic apparatus during frequent and rapid activation by humid air on clear mornings. PMID:22762452

Insensitivity to Flaws Leads to Damage Tolerance in Brittle Architected Meta-Materials

NASA Astrophysics Data System (ADS)

Montemayor, L. C.; Wong, W. H.; Zhang, Y.-W.; Greer, J. R.

2016-02-01

Cellular solids are instrumental in creating lightweight, strong, and damage-tolerant engineering materials. By extending feature size down to the nanoscale, we simultaneously exploit the architecture and material size effects to substantially enhance structural integrity of architected meta-materials. We discovered that hollow-tube alumina nanolattices with 3D kagome geometry that contained pre-fabricated flaws always failed at the same load as the pristine specimens when the ratio of notch length (a) to sample width (w) is no greater than 1/3, with no correlation between failure occurring at or away from the notch. Samples with (a/w) > 0.3, and notch length-to-unit cell size ratios of (a/l) > 5.2, failed at a lower peak loads because of the higher sample compliance when fewer unit cells span the intact region. Finite element simulations show that the failure is governed by purely tensile loading for (a/w) < 0.3 for the same (a/l); bending begins to play a significant role in failure as (a/w) increases. This experimental and computational work demonstrates that the discrete-continuum duality of architected structural meta-materials may give rise to their damage tolerance and insensitivity of failure to the presence of flaws even when made entirely of intrinsically brittle materials.

Welded-woven fabrics for use as synthetic, minimally invasive orthopaedic implants

NASA Astrophysics Data System (ADS)

Rodts, Timothy W.

The treatment of osteoarthritis in healthcare today focuses on minimizing pain and retaining mobility. Osteoarthritis of the knee is a common disease and known to be associated with traumatic injuries, among other factors. An identified trend is that patients are younger and have expectations of life with the preservation of an active lifestyle. As a result, great strain is placed on the available offerings of healthcare professionals and device manufacturers alike. This results in numerous design challenges for managing pain and disease over an extended period of time. The available treatments are being extended into younger populations, which increasingly suffer traumatic knee injuries. However, these patients are not good candidates for total joint replacement. A common problem for young patients is localized cartilage damage. This can heal, but often results in a painful condition that requires intervention. A welded-woven three-dimensional polymer fabric was developed to mimic the properties of articular cartilage. A process for the laser welding reinforcement of the surface layers of three-dimensional fabrics was investigated. Confined compression creep and pin-on-disc wear studies were conducted to characterize the contribution of the surface welding reinforcement. All materials used in the studies have previously been used in orthopaedic devices or meet the requirements for United States Pharmacopeial Convention (USP) Class VI biocompatibility approval. The compressive behavior of three-dimensional fabrics was tailored by the inclusion of surface welds. The compressive properties of the welded-woven fabrics were shown to better approximate articular cartilage compressive properties than conventional woven materials. The wear performance was benchmarked against identical fabrics without welding reinforcement. The wear rates were significantly reduced and the lifespan of the fabrics was markedly improved due to surface welding. Welding reinforcement offers a strengthening mechanism as well as a damage-resistant and damage-tolerant treatment for three-dimensional fabrics. Additionally, the concept of reinforcing three-dimensional fabrics in general has been proven and is transferrable to many industries and applications. The manufacturing approaches are scalable and robust.

Association mapping provides insights into the origin and the fine structure of the sorghum aluminum tolerance locus, AltSB

USDA-ARS?s Scientific Manuscript database

Root damage caused by aluminum (Al) toxicity is a major cause of grain yield reduction on acid soils, which are prevalent in tropical and subtropical regions of the world where food security is most tenuous. In sorghum, Al tolerance is conferred by SbMATE, an Al-activated root citrate efflux transpo...

Development of powder metallurgy 2XXX series Al alloys for high temperature aircraft structural applications

NASA Technical Reports Server (NTRS)

Chellman, D. J.

1984-01-01

The objective of the present investigation was to improve the strength and fracture toughness combination of P/M 2124 Al alloys in accordance with NASA program goals for damage tolerance and fatigue resistance. Two (2) P/M compositions based on Al-3.70 Cu-1.85 Mg-0.20 Mn with 0.12 and 0.60 wt. pct. Zr were selected for investigation. The rapid solidification rates produced by atomization were observed to prohibit the precipitation of coarse, primary Al3Zr in both alloys. A major portion of the Zr precipitated as finely distributed, coherent Al3Zr phases during vacuum preheating and solution heat treatment. The proper balance between Cu and Mg contents eliminated undissolved, soluble constituents such as Al2CuMg and Al2Cu during atomization. The resultant extruded microstructures produced a unique combination of strength and fracture toughness. An increase in the volume fraction of coherent Al3Zr, unlike incoherent Al20Cu2Mn3 dispersoids, strengthened the P/M Al base alloy either directly by dislocation-precipitate interactions, indirectly by a retardation of recrystallization, or a combination of both mechanisms. Furthermore, coherent Al3Zr does not appear to degrade toughness to the extent that incoherent Al20Cu2Mn3 does. Consequently, the addition of 0.60 wt. pct. Zr to the base alloy, incorporated with a 774K (935 F) solution heat treatment temperature, produces an alloy which exceeds all tensile property and fracture toughness goals for damage tolerant and fatigue resistant applications in the naturally aged condition.

NASA workshop on impact damage to composites

NASA Technical Reports Server (NTRS)

Poe, C. C., Jr.

1991-01-01

A compilation of slides presented at the NASA Workshop on Impact Damage to Composites held on March 19 and 20, 1991, at the Langley Research Center, Hampton, Virginia is given. The objective of the workshop was to review technology for evaluating impact damage tolerance of composite structures and identify deficiencies. Research, development, design methods, and design criteria were addressed. Actions to eliminate technology deficiencies were developed. A list of those actions and a list of attendees are also included.

Specialists Meeting on Impact Damage Tolerance of Structures

DTIC Science & Technology

1976-01-01

example, fatigue, timl-de tectIable initial defects and in-fliglht d amalt such aS that inflicted by miilitary weapons or by debris from ’n din tegra t...relative to many types of damaging mechanisms, lncludlig for example: I. Fat Igue 2. Non-detectable Initial defects 3. In-flight damage, such as Inflicted...undetected flaw or defect . In both cases, the benefits of successful design are Improved safety and economics. With respect to In-flight darvqe, tre

Fracture and crack growth in orthotropic laminates

NASA Technical Reports Server (NTRS)

Goree, James G.; Kaw, Autar K.

1985-01-01

A mathematical model based on the classical shear-lag assumptions is used to study the residual strength and fracture behavior of composite laminates with symmetrically placed buffer strips. The laminate is loaded by a uniform remote longitudinal tensile strain and has initial damage in the form of a transverse crack in the parent laminate between buffer strips. The crack growth behavior as a function of material properties, number of buffer-strip plies, spacing, width of buffer strips, longitudinal matrix splitting, and debonding at the interface is studied. Buffer-strip laminates are shown to arrest fracture and increase the residual strengths significantly over those of one material laminates, with S-glass being a more effective buffer strip material than Kevlar in increasing the damage tolerance of graphite/epoxy panels. For a typical graphite/epoxy laminate with S-glass buffer-strips, the residual strength is about 2.4 times the residual strength of an all graphite/epoxy panel with the same crack length. Approximately 50% of this increase is due to the S-glass/epoxy buffer-strips, 40% due to longitudinal splitting of the buffer strip interface and 10% due to bonding.

A Numerical Study on the Edgewise Compression Strength of Sandwich Structures with Facesheet-Core Disbonds

NASA Technical Reports Server (NTRS)

Bergan, Andrew C.

2017-01-01

Damage tolerant design approaches require determination of critical damage modes and flaw sizes in order to establish nondestructive evaluation detection requirements. A finite element model is developed to assess the effect of circular facesheet-core disbonds on the strength of sandwich specimens subjected to edgewise compressive loads for the purpose of predicting the critical flaw size for a variety of design parameters. Postbuckling analyses are conducted in which an initial imperfection is seeded using results from a linear buckling analysis. Both the virtual crack closure technique (VCCT) and cohesive elements are considered for modeling disbond growth. Predictions from analyses using the VCCT and analyses using cohesive elements are in good correlation. A series of parametric analyses are conducted to investigate the effect of core thickness and material, facesheet layup, facesheet-core interface properties, and curvature on the criticality of facesheet-core disbonds of various sizes. The results from these analyses provide a basis for determining the critical flaw size for facesheet-core disbonds subjected to edgewise compression loads and, therefore, nondestructive evaluation flaw detection requirements for this configuration.

Impact face influence on low velocity impact performance of interply laminated plates

NASA Astrophysics Data System (ADS)

Manikandan, Periyasamy; Chai, Gin Boay

2015-03-01

Fibre Metal Laminate (FML), a metal sandwiched hybrid composite material is well-known for its enhanced impact properties and better damage tolerance and it has been successfully implemented in diverse engineering applications in aviation industry. With heterogeneous constituents, the stacking sequence of FML is believe to play a critical role to govern its overall energy absorption capability by means of controlling delamination of metal composite interface and plastic deformation of metal layers. As a precursor, low velocity impact experiments were conducted on interply configured transparent plastic plates in order to extract the significance of stacking sequence and realize the characteristics of each layer through naked eye which is not possible in FML due to opacity of metal layer. The stack configuration constitute hard acrylic (brittle) and soft polycarbonate (ductile) plates analogous to composite (brittle) and metal (ductile) layers on FML laminate and the impact event is performed on either hard or soft facing sides separately. Hard side samples resemble more protective than soft side impact sample, with large peak resistant force and expose smaller damage growth in all experimented cases.

Hematopoietic tissue repair under chronic low daily dose irradiation

NASA Astrophysics Data System (ADS)

Seed, T. M.

The capacity of the hematopoietic system to repair constantly accruing cellular damage under chronic, low daily dose gamma irradiation is essential for the maintenance of a functional hematopoietic system, and, in turn, long term survival. In certain individuals, however, such continuous cycles of damage and repair provide an essential inductive environment for selected types of hematopathologies, e.g., myeloid leukemia (ML). In our laboratory we have been studying temporal and causal relationships between hematopoietic capacity, associated repair functions, and propensities for hematologic disease in canines under variable levels of chronic radiation stress (0.3-26.3 cGy d^-1). Results indicate that the maximum exposure rate tolerated by the hematopoietic system is highly individual-specific (three major responding subgroups identified) and is based largely on the degree to which repair capacity, and, in turn, hematopoietic restoration, is augmented under chronic exposure. In low-tolerance individuals (prone to aplastic anemia, subgroup 1), the failure to augment basic repair functions seemingly results in a progressive accumulation of genetic and cellular damage within vital progenitorial marrow compartments (particularly marked within erythroid compartments) that results in loss of reproductive capacity and ultimately in collapse of the hematopoietic system. The high-tolerance individuals (radioaccommodated and either prone- or not prone to ML, subgroup 2 & 3) appear to minimize the accumulating damage effect of daily exposures by extending repair functions, which preserves reproductive integrity and fosters regenerative hematopoietic responses. As the strength of the regenerative response manifests the extent of repair augmentation, the relatively strong response of high-tolerance individuals progressing to patent ML suggests an insufficiency of repair quality rather than repair quantity. The kinetics of these repair-mediated, regenerative hematopoietic responses within the major subgroups are under study and should provide useful insights into the nature of hematopoietic accommodation (or its failure) under greatly extended periods of chronic, low-daily-dose ionizing radiation exposure.

Removal of nutrient limitations in forest gaps enhances growth rate and resistance to cavitation in subtropical canopy tree species differing in shade tolerance.

PubMed

Villagra, Mariana; Campanello, Paula I; Montti, Lia; Goldstein, Guillermo

2013-03-01

A 4-year fertilization experiment with nitrogen (N) and phosphorus (P) was carried out in natural gaps of a subtropical forest in northeastern Argentina. Saplings of six dominant canopy species differing in shade tolerance were grown in five control and five N + P fertilized gaps. Hydraulic architectural traits such as wood density, the leaf area to sapwood area ratio (LA : SA), vulnerability to cavitation (P50) and specific and leaf-specific hydraulic conductivity were measured, as well as the relative growth rate, specific leaf area (SLA) and percentage of leaf damage by insect herbivores. Plant growth rates and resistance to drought-induced embolisms increased when nutrient limitations were removed. On average, the P50 of control plants was -1.1 MPa, while the P50 of fertilized plants was -1.6 MPa. Wood density and LA : SA decreased with N + P additions. A trade-off between vulnerability to cavitation and efficiency of water transport was not observed. The relative growth rate was positively related to the total leaf surface area per plant and negatively related to LA : SA, while P50 was positively related to SLA across species and treatments. Plants with higher growth rates and higher total leaf area in fertilized plots were able to avoid hydraulic dysfunction by becoming less vulnerable to cavitation (more negative P50). Two high-light-requiring species exhibited relatively low growth rates due to heavy herbivore damage. Contrary to expectations, shade-tolerant plants with relatively high resistance to hydraulic dysfunction and reduced herbivory damage were able to grow faster. These results suggest that during the initial phase of sapling establishment in gaps, species that were less vulnerable to cavitation and exhibited reduced herbivory damage had faster realized growth rates than less shade-tolerant species with higher potential growth rates. Finally, functional relationships between hydraulic traits and growth rate across species and treatments were maintained regardless of soil nutrient status.

Genome-wide map of Apn1 binding sites under oxidative stress in Saccharomyces cerevisiae.

PubMed

Morris, Lydia P; Conley, Andrew B; Degtyareva, Natalya; Jordan, I King; Doetsch, Paul W

2017-11-01

The DNA is cells is continuously exposed to reactive oxygen species resulting in toxic and mutagenic DNA damage. Although the repair of oxidative DNA damage occurs primarily through the base excision repair (BER) pathway, the nucleotide excision repair (NER) pathway processes some of the same lesions. In addition, damage tolerance mechanisms, such as recombination and translesion synthesis, enable cells to tolerate oxidative DNA damage, especially when BER and NER capacities are exceeded. Thus, disruption of BER alone or disruption of BER and NER in Saccharomyces cerevisiae leads to increased mutations as well as large-scale genomic rearrangements. Previous studies demonstrated that a particular region of chromosome II is susceptible to chronic oxidative stress-induced chromosomal rearrangements, suggesting the existence of DNA damage and/or DNA repair hotspots. Here we investigated the relationship between oxidative damage and genomic instability utilizing chromatin immunoprecipitation combined with DNA microarray technology to profile DNA repair sites along yeast chromosomes under different oxidative stress conditions. We targeted the major yeast AP endonuclease Apn1 as a representative BER protein. Our results indicate that Apn1 target sequences are enriched for cytosine and guanine nucleotides. We predict that BER protects these sites in the genome because guanines and cytosines are thought to be especially susceptible to oxidative attack, thereby preventing large-scale genome destabilization from chronic accumulation of DNA damage. Information from our studies should provide insight into how regional deployment of oxidative DNA damage management systems along chromosomes protects against large-scale rearrangements. Copyright © 2017 John Wiley & Sons, Ltd. Copyright © 2017 John Wiley & Sons, Ltd.

Mechanisms of Oryza sativa (Poaceae) resistance to Tagosodes orizicolus (Homoptera: Delphacidae) under greenhouse condition in Venezuela.

PubMed

González, Alex; Labrín, Natalia; Alvarez, Rosa M; Jayaro, Yorman; Gamboa, Carlos; Reyes, Edicta; Barrientos, Venancio

2012-03-01

Tagosodes orizicolus is one of the main plagues of rice in tropical America causing two types of damages, the direct one, feeding and oviposition effect, and an indirect one, by the transmission of the "Rice hoja blanca virus". During 2006-2007 we carried out research under greenhouse conditions at Fundaci6n Danac, Venezuela, in order to determine the mechanisms of antixenosis, antibiosis and tolerance to T. orizicolus, which could be acting in commercial varieties and advanced lines of the rice genetic breeding programs of INIA and Fundaci6n Danac. The method of free feeding was used for the antixenosis evaluation, whereas the method of forced feeding was used for antibiosis evaluation (effect on survival and oviposition). Additionally, we used the indirect method based on biomass depression to estimate the tolerance. Some of the evaluated traits included: grade of damage, number of insects settling on rice plants, percentage of sogata mortality at the mature state, number of eggs in the leaf midrib and an index of tolerance. The results showed that rice genotypes possess different combinations of resistance mechanisms, as well as different grades of reactions. The susceptible control 'Bluebonnet 50' was consistently susceptible across experiments and the resistant control 'Makalioka' had high antixenosis and high antibiosis based on survival and oviposition. The rest of the genotypes presented lower or higher degrees of antixenosis and antibiosis for survival and oviposition. The genotype 'FD0241-M-17-6-1-1-1-1' was identified with possible tolerance to the direct damage of sogata.

Solidago gigantea plants from nonnative ranges compensate more in response to damage than plants from the native range.

PubMed

Liao, Huixuan; Gurgel, Priscila C S; Pal, Robert W; Hooper, David; Callaway, Ragan M

2016-09-01

Resistance and tolerance are two ways that plants cope with herbivory. Tolerance, the ability of a plant to regrow or reproduce after being consumed, has been studied less than resistance, but this trait varies widely among species and has considerable potential to affect the ecology of plant species. One particular aspect of tolerance, compensatory responses, can evolve rapidly in plant species; providing insight into interactions between consumers and plants. However, compensation by invasive species has rarely been explored. We compared compensatory responses to the effects of simulated herbivory expressed by plants from seven Solidago gigantea populations from the native North American range to that expressed by plants from nine populations from the nonnative European range. Populations were also collected along elevational gradients to compare ecotypic variation within and between ranges. Solidago plants from the nonnative range of Europe were more tolerant to herbivory than plants from the native range of North America. Furthermore, plants from European populations increased in total biomass and growth rate with elevation, but decreased in compensatory response. There were no relationships between elevation and growth or compensation for North American populations. Our results suggest that Solidago gigantea may have evolved to better compensate for herbivory damage in Europe, perhaps in response to a shift to greater proportion of attack from generalists. Our results also suggest a possible trade-off between rapid growth and compensation to damage in European populations but not in North American populations. © 2016 by the Ecological Society of America.

Physiochemical properties and application of hyaluronic acid: a systematic review.

PubMed

Salwowska, Natalia M; Bebenek, Katarzyna A; Żądło, Dominika A; Wcisło-Dziadecka, Dominika L

2016-12-01

Hyaluronic acid is a widely available, biocompatible, polysaccharide with distinguishing physiochemical properties which inspire its application throughout several fields of medicine. We aim to investigate the application of hyaluronic acid and its effectiveness throughout several fields of medicine, including several therapies administered and prescribed by general health practitioners. We conducted a systematic review on randomized controlled trials about the physiochemical properties of hyaluronic acid and its application through primary care. Studies included in this review were peer reviewed and met our inclusion criteria. Factors were clustered into the following: uses throughout several fields of medicine, physiochemical properties, bioavailability, tolerance, effectiveness, and adverse effects. Therapies with hyaluronic acid provided long-lasting, pain relieving, moisturizing, lubricating, and dermal filling effect. Tissue hydration, elasticity, and durability improved. Adjunct therapy with hyaluronic acid provides longer-lasting therapeutic effect when compared to the use of glucocorticosteroids and NSAIDs in osteoarthritic chronic diseases, is well-established in ophthalmology due to its lubricating properties for the corneal endothelium, and improves tissue hydration and cellular resistance to mechanical damage in aesthetic dermatology, and has marginal adverse effects. Several trials indicated its role in tumor markers, liver diseases, and in pharmaceuticals, but further research would be necessary to draw conclusive results in those fields. © 2016 Wiley Periodicals, Inc.

New iron-sulfur clusters help hydrogenases tolerate oxygen.

PubMed

Grubel, Katarzyna; Holland, Patrick L

2012-04-02

One S less: recent crystallographic studies have revealed a new, oxygen-tolerant kind of iron-sulfide cluster [4Fe-3S], which contains only three rather than four sulfur atoms in its cage (see picture; yellow=S, red=Fe, blue=N, green=cysteine). It is proposed that the cluster's ability to transfer multiple electrons increases the oxygen tolerance by enabling the enzyme to reduce O(2) rapidly, converting the dioxygen into harmless water before it can damage the protein. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Preparation and Exceptional Mechanical Properties of Bone-Mimicking Size-Tuned Graphene Oxide@Carbon Nanotube Hybrid Paper.

PubMed

Oh, Jun Young; Kim, Yern Seung; Jung, Yeonsu; Yang, Seung Jae; Park, Chong Rae

2016-02-23

The self-assembled nanostructures of carbon nanomaterials possess a damage-tolerable architecture crucial for the inherent mechanical properties at both micro- and macroscopic levels. Bone, or "natural composite," has been known to have superior energy dissipation and fracture resistance abilities due to its unique load-bearing hybrid structure. However, few approaches have emulated the desirable structure using carbon nanomaterials. In this paper, we present an approach in fabricating a hybrid composite paper based on graphene oxide (GO) and carbon nanotube (CNT) that mimicks the natural bone structure. The size-tuning strategy enables smaller GO sheets to have more cross-linking reactions with CNTs and be homogeneously incorporated into CNT-assembled paper, which is advantageous for effective stress transfer. The resultant hybrid composite film has enhanced mechanical strength, modulus, toughness, and even electrical conductivity compared to previously reported CNT-GO based composites. We further demonstrate the usefulness of the size-tuned GOs as the "stress transfer medium" by performing in situ Raman spectroscopy during the tensile test.

Flaw Tolerance In Lap Shear Brazed Joints. Part 2

NASA Technical Reports Server (NTRS)

Wang, Len; Flom, Yury

2003-01-01

This paper presents results of the second part of an on-going effort to gain better understanding of defect tolerance in braze joints. In the first part of this three-part series, we mechanically tested and modeled the strength of the lap joints as a function of the overlap distance. A failure criterion was established based on the zone damage theory, which predicts the dependence of the lap joint shear strength on the overlap distance, based on the critical size of a finite damage zone or an overloaded region in the joint. In this second part of the study, we experimentally verified the applicability of the damage zone criterion on prediction of the shear strength of the lap joint and introduced controlled flaws into the lap joints. The purpose of the study was to evaluate the lap joint strength as a function of flaw size and its location through mechanical testing and nonlinear finite element analysis (FEA) employing damage zone criterion for definition of failure. The results obtained from the second part of the investigation confirmed that the failure of the ductile lap shear brazed joints occurs when the damage zone reaches approximately 10% of the overlap width. The same failure criterion was applicable to the lap joints containing flaws.

The importance of fracture toughness in ultrafine and nanocrystalline bulk materials

PubMed Central

Pippan, R.; Hohenwarter, A.

2016-01-01

ABSTRACT The suitability of high-strength ultrafine and nanocrystalline materials processed by severe plastic deformation methods and aimed to be used for structural applications will strongly depend on their resistance against crack growth. In this contribution some general available findings on the damage tolerance of this material class will be summarized. Particularly, the occurrence of a pronounced fracture anisotropy will be in the center of discussion. In addition, the great potential of this generated anisotropy to obtain high-strength materials with exceptionally high fracture toughness in specific loading and crack growth directions will be enlightened. IMPACT STATEMENT Severely plastically deformed materials are reviewed in light of their damage tolerance. The frequently observed toughness anisotropy allows unprecedented fracture toughness – strength combinations. PMID:27570712

Advances in Composites Technology

NASA Technical Reports Server (NTRS)

Tenney, D. R.; Dexter, H. B.

1985-01-01

A significant level of research is currently focused on the development of tough resins and high strain fibers in an effort to gain improved damage tolerance. Moderate success has been achieved with the development of new resins such as PEEK and additional improvements look promising with new thermoplastic resins. Development of innovative material forms such as 2-D and 3-D woven fabrics and braided structural subelements is also expected to improve damage tolerance and durability of composite hardware. The new thrust in composites is to develop low cost manufacturing and design concepts to lower the cost of composite hardware. Processes being examined include automated material placement, filament winding, pultrusion, and thermoforming. The factory of the future will likely incorporate extensive automation in all aspects of manufacturing composite components.

[The immune system and the eye].

PubMed

Faber, Carsten; Nissen, Mogens Holst

2008-09-15

The special relationship between the eye and the immune system rests on a number of anatomical, physiological and immunological mechanisms. These mechanisms prevent the delicate structures of the eye from potentially damaging immunogenic inflammation while protecting against pathogens. Rather than inflammation, antigen induces a form of systemic and antigen-specific immunological tolerance. Owing to its systemic nature, this tolerance may be utilised to achieve successful treatment of immunological disorders.

Tolerance to multiple climate stressors: a case study of Douglas-fir drought and cold hardiness

Treesearch

Sheel Bansal; Connie Harrington; Brad St. Clair

2016-01-01

1. Drought and freeze events are two of the most common forms of climate extremes which result in tree damage or death, and the frequency and intensity of both stressors may increase with climate change. Few studies have examined natural covariation in stress tolerance traits to cope with multiple stressors among wild plant populations. 2. We assessed the...

On the Influence of Nb/Ti Ratio on Environmentally-Assisted Crack Growth in High-Strength Nickel-Based Superalloys

NASA Astrophysics Data System (ADS)

Németh, A. A. N.; Crudden, D. J.; Collins, D. M.; Kuksenko, V.; Liebscher, C. H.; Armstrong, D. E. J.; Wilkinson, A. J.; Reed, R. C.

2018-05-01

The effect of Nb/Ti ratio on environmentally-assisted crack growth of three prototype Ni-based superalloys is studied. For these alloys, the yield strength is unaltered with increasing Nb/Ti ratio due to an increase in grain size. This situation has allowed the rationalization of the factors influencing damage tolerance at 700 °C. Primary intergranular cracks have been investigated using energy-dispersive X-ray spectroscopy in a scanning transmission electron microscope and the analysis of electron back-scatter diffraction patterns. Any possible detrimental effect of Nb on the observed crack tip damage due to Nb-rich oxide formation is not observed. Instead, evidence is presented to indicate that the tertiary γ'-precipitates are dissolving ahead of the crack consistent with the formation of oxides such as alumina and rutile. Our results have implications for alloy design efforts; at any given strength level, both more and less damage-tolerant variants of these alloys can be designed.

Damage tolerance of nuclear graphite at elevated temperatures

DOE PAGES

Liu, Dong; Gludovatz, Bernd; Barnard, Harold S.; ...

2017-06-30

Nuclear-grade graphite is a critically important high-temperature structural material for current and potentially next generation of fission reactors worldwide. It is imperative to understand its damage-tolerant behaviour and to discern the mechanisms of damage evolution under in-service conditions. Here we perform in situ mechanical testing with synchrotron X-ray computed micro-tomography at temperatures between ambient and 1,000 °C on a nuclear-grade Gilsocarbon graphite. We find that both the strength and fracture toughness of this graphite are improved at elevated temperature. Whereas this behaviour is consistent with observations of the closure of microcracks formed parallel to the covalent-sp 2-bonded graphene layers atmore » higher temperatures, which accommodate the more than tenfold larger thermal expansion perpendicular to these layers, we attribute the elevation in strength and toughness primarily to changes in the residual stress state at 800–1,000 °C, specifically to the reduction in significant levels of residual tensile stresses in the graphite that are ‘frozen-in’ following processing.« less

Damage tolerance of nuclear graphite at elevated temperatures

PubMed Central

Liu, Dong; Gludovatz, Bernd; Barnard, Harold S.; Kuball, Martin; Ritchie, Robert O.

2017-01-01

Nuclear-grade graphite is a critically important high-temperature structural material for current and potentially next generation of fission reactors worldwide. It is imperative to understand its damage-tolerant behaviour and to discern the mechanisms of damage evolution under in-service conditions. Here we perform in situ mechanical testing with synchrotron X-ray computed micro-tomography at temperatures between ambient and 1,000 °C on a nuclear-grade Gilsocarbon graphite. We find that both the strength and fracture toughness of this graphite are improved at elevated temperature. Whereas this behaviour is consistent with observations of the closure of microcracks formed parallel to the covalent-sp2-bonded graphene layers at higher temperatures, which accommodate the more than tenfold larger thermal expansion perpendicular to these layers, we attribute the elevation in strength and toughness primarily to changes in the residual stress state at 800–1,000 °C, specifically to the reduction in significant levels of residual tensile stresses in the graphite that are ‘frozen-in’ following processing. PMID:28665405

Evolution of resistance and tolerance to herbivores: testing the trade-off hypothesis.

PubMed

Kariñho-Betancourt, Eunice; Núñez-Farfán, Juan

2015-01-01

Background. To cope with their natural enemies, plants rely on resistance and tolerance as defensive strategies. Evolution of these strategies among natural population can be constrained by the absence of genetic variation or because of the antagonistic genetic correlation (trade-off) between them. Also, since plant defenses are integrated by several traits, it has been suggested that trade-offs might occur between specific defense traits. Methodology/Principal Findings. We experimentally assessed (1) the presence of genetic variance in tolerance, total resistance, and leaf trichome density as specific defense trait, (2) the extent of natural selection acting on plant defenses, and (3) the relationship between total resistance and leaf trichome density with tolerance to herbivory in the annual herb Datura stramonium. Full-sib families of D. stramonium were either exposed to natural herbivores (control) or protected from them by a systemic insecticide. We detected genetic variance for leaf trichome density, and directional selection acting on this character. However, we did not detect a negative significant correlation between tolerance and total resistance, or between tolerance and leaf trichome density. We argue that low levels of leaf damage by herbivores precluded the detection of a negative genetic correlation between plant defense strategies. Conclusions/Significance. This study provides empirical evidence of the independent evolution of plant defense strategies, and a defensive role of leaf trichomes. The pattern of selection should favor individuals with high trichomes density. Also, because leaf trichome density reduces damage by herbivores and possess genetic variance in the studied population, its evolution is not constrained.

Evolution of resistance and tolerance to herbivores: testing the trade-off hypothesis

PubMed Central

Kariñho-Betancourt, Eunice

2015-01-01

Background. To cope with their natural enemies, plants rely on resistance and tolerance as defensive strategies. Evolution of these strategies among natural population can be constrained by the absence of genetic variation or because of the antagonistic genetic correlation (trade-off) between them. Also, since plant defenses are integrated by several traits, it has been suggested that trade-offs might occur between specific defense traits. Methodology/Principal Findings. We experimentally assessed (1) the presence of genetic variance in tolerance, total resistance, and leaf trichome density as specific defense trait, (2) the extent of natural selection acting on plant defenses, and (3) the relationship between total resistance and leaf trichome density with tolerance to herbivory in the annual herb Datura stramonium. Full-sib families of D. stramonium were either exposed to natural herbivores (control) or protected from them by a systemic insecticide. We detected genetic variance for leaf trichome density, and directional selection acting on this character. However, we did not detect a negative significant correlation between tolerance and total resistance, or between tolerance and leaf trichome density. We argue that low levels of leaf damage by herbivores precluded the detection of a negative genetic correlation between plant defense strategies. Conclusions/Significance. This study provides empirical evidence of the independent evolution of plant defense strategies, and a defensive role of leaf trichomes. The pattern of selection should favor individuals with high trichomes density. Also, because leaf trichome density reduces damage by herbivores and possess genetic variance in the studied population, its evolution is not constrained. PMID:25780756

On the radiation tolerance of SU-8, a new material for gaseous microstructure radiation detector fabrication

NASA Astrophysics Data System (ADS)

Key, M. J.; Cindro, V.; Lozano, M.

2004-12-01

SU-8 photosensitive epoxy resin was developed for the fabrication of high-aspect ratio microstructures in MEMS and microengineering applications, and has potential for use in the construction of novel gaseous micropattern radiation detectors. However, little is known of the behaviour of the cured material under irradiation. Mechanical properties of SU-8 film have been measured as a function of neutron exposure and compared with Kapton ® polyimide and Mylar ® PET polyester films, materials routinely used in gaseous radiation detectors, to asses the suitability of SU-8 based microstructures for gaseous detector applications. After exposure to a reactor core neutron fluence of 7.5×10 18 n cm -2, the new material showed a high level of resistance to radiation damage, comparable to Kapton film.

A novel biomimetic approach to the design of high-performance ceramic/metal composites

DOE Office of Scientific and Technical Information (OSTI.GOV)

Launey, Maximilien E.; Munch, Etienne; Alsem, Daan Hein

2009-08-01

The prospect of extending natural biological design to develop new synthetic ceramic-metal composite materials is examined. Using ice-templating of ceramic suspensions and subsequent metal infiltration, we demonstrate that the concept of ordered hierarchical design can be applied to create fine-scale laminated ceramic-metal (bulk) composites that are inexpensive, lightweight and display exceptional damage-tolerance properties. Specifically, Al{sub 2}O{sub 3}/Al-Si laminates with ceramic contents up to approximately 40 vol% and with lamellae thicknesses down to 10 {micro}m were processed and characterized. These structures achieve an excellent fracture toughness of 40 MPa{radical}m at a tensile strength of approximately 300 MPa. Salient toughening mechanisms aremore » described together with further toughening strategies.« less

Tough, processable semi-interpenetrating polymer networks from monomer reactants

NASA Technical Reports Server (NTRS)

Pater, Ruth H. (Inventor)

1994-01-01

A high temperature semi-interpenetrating polymer network (semi-IPN) was developed which had significantly improved processability, damage tolerance, and mechanical performance, when compared to the commercial Thermid materials. This simultaneous semi-IPN was prepared by mixing the monomer precursors of Thermid AL-600 (a thermoset) and NR-150B2 (a thermoplastic) and allowing the monomers to react randomly upon heating. This reaction occurs at a rate which decreases the flow and broadens the processing window. Upon heating at a higher temperature, there is an increase in flow. Because of the improved flow properties, broadened processing window and enhanced toughness, high strength polymer matrix composites, adhesives and molded articles can now be prepared from the acetylene end-capped polyimides which were previously inherently brittle and difficult to process.

Assessment of impact damage of composite rocket motor cases

NASA Technical Reports Server (NTRS)

Paris, Henry G.

1994-01-01

This contract reviewed the available literature on mechanisms of low velocity impact damage in filament wound rocket motor cases, MDE methods to quantify damage, critical coupon level test methods, manufacturing and material process variables and empirical and analytical modeling off impact damage. The critical design properties for rocket motor cases are biaxial hoop and axial tensile strength. Low velocity impact damage is insidious because it can create serious nonvisible damage at very low impact velocities. In thick rocket motor cases the prevalent low velocity impact damage is fiber fracture and matrix cracking adjacent to the front face. In contrast, low velocity loading of thin wall cylinders induces flexure, depending on span length and the flexure induces delamination and tensile cracking on the back face wall opposed to impact occurs due to flexural stresses imposed by impact loading. Important NDE methods for rocket motor cases are non-contacting methods that allow inspection from one side. Among these are vibrothermography, and pulse-echo methods based on acoustic-ultrasonic methods. High resolution techniques such as x-ray computed tomography appear to have merit for accurate geometrical characterization of local damage to support development of analytical models of micromechanics. The challenge of coupon level testing is to reproduce the biaxial stress state that the full scale article experiences, and to determine how to scale the composite structure to model full sized behavior. Biaxial tensile testing has been performed by uniaxially tensile loading internally pressurized cylinders. This is experimentally difficult due to gripping problems and pressure containment. Much prior work focused on uniaxial tensile testing of model filament wound cylinders. Interpretation of the results of some studies is complicated by the fact that the fabrication process did not duplicate full scale manufacturing. It is difficult to scale results from testing subscale cylinders since there are significant differences in out time of the resins relative to full scale cylinder fabrication, differences in hoop fiber tensioning and unsatisfactory coupon configurations. It appears that development of a new test method for subscale cylinders is merited. Damage tolerance may be improved by material optimization that uses fiber treatments and matrix modifications to control the fiber matrix interface bonding. It is difficult to develop process optimization in subscale cylinders without also modeling the longer out times resins experience in full scale testing. A major breakthrough in characterizing the effect of impact damage on residual strength, and understanding how to scale results of subscale evaluations, will be a sound micromechanical model that described progressive failure of the composite. Such models will utilize a three dimensional stress analysis due to the complex nature of low velocity impact stresses in thick composites. When these models are coupled with non-contact NDE methods that geometrically characterize the damage and acoustic methods that characterize the effective local elastic properties, accurate assessment of residual strength from impact damage may be possible. Directions for further development are suggested.

Assessment of impact damage of composite rocket motor cases

NASA Astrophysics Data System (ADS)

Paris, Henry G.

1994-02-01

This contract reviewed the available literature on mechanisms of low velocity impact damage in filament wound rocket motor cases, MDE methods to quantify damage, critical coupon level test methods, manufacturing and material process variables and empirical and analytical modeling off impact damage. The critical design properties for rocket motor cases are biaxial hoop and axial tensile strength. Low velocity impact damage is insidious because it can create serious nonvisible damage at very low impact velocities. In thick rocket motor cases the prevalent low velocity impact damage is fiber fracture and matrix cracking adjacent to the front face. In contrast, low velocity loading of thin wall cylinders induces flexure, depending on span length and the flexure induces delamination and tensile cracking on the back face wall opposed to impact occurs due to flexural stresses imposed by impact loading. Important NDE methods for rocket motor cases are non-contacting methods that allow inspection from one side. Among these are vibrothermography, and pulse-echo methods based on acoustic-ultrasonic methods. High resolution techniques such as x-ray computed tomography appear to have merit for accurate geometrical characterization of local damage to support development of analytical models of micromechanics. The challenge of coupon level testing is to reproduce the biaxial stress state that the full scale article experiences, and to determine how to scale the composite structure to model full sized behavior. Biaxial tensile testing has been performed by uniaxially tensile loading internally pressurized cylinders. This is experimentally difficult due to gripping problems and pressure containment. Much prior work focused on uniaxial tensile testing of model filament wound cylinders. Interpretation of the results of some studies is complicated by the fact that the fabrication process did not duplicate full scale manufacturing. It is difficult to scale results from testing subscale cylinders since there are significant differences in out time of the resins relative to full scale cylinder fabrication, differences in hoop fiber tensioning and unsatisfactory coupon configurations. It appears that development of a new test method for subscale cylinders is merited. Damage tolerance may be improved by material optimization that uses fiber treatments and matrix modifications to control the fiber matrix interface bonding. It is difficult to develop process optimization in subscale cylinders without also modeling the longer out times resins experience in full scale testing. A major breakthrough in characterizing the effect of impact damage on residual strength, and understanding how to scale results of subscale evaluations, will be a sound micromechanical model that described progressive failure of the composite.

Balancing repair and tolerance of DNA damage caused by alkylating agents.

PubMed

Fu, Dragony; Calvo, Jennifer A; Samson, Leona D

2012-01-12

Alkylating agents constitute a major class of frontline chemotherapeutic drugs that inflict cytotoxic DNA damage as their main mode of action, in addition to collateral mutagenic damage. Numerous cellular pathways, including direct DNA damage reversal, base excision repair (BER) and mismatch repair (MMR), respond to alkylation damage to defend against alkylation-induced cell death or mutation. However, maintaining a proper balance of activity both within and between these pathways is crucial for a favourable response of an organism to alkylating agents. Furthermore, the response of an individual to alkylating agents can vary considerably from tissue to tissue and from person to person, pointing to genetic and epigenetic mechanisms that modulate alkylating agent toxicity.

SERIES: Genomic instability in cancer Balancing repair and tolerance of DNA damage caused by alkylating agents

PubMed Central

Fu, Dragony; Calvo, Jennifer A.; Samson, Leona D

2013-01-01

Alkylating agents comprise a major class of frontline chemotherapeutic drugs that inflict cytotoxic DNA damage as their main mode of action, in addition to collateral mutagenic damage. Numerous cellular pathways, including direct DNA damage reversal, base excision repair (BER), and mismatch repair (MMR) respond to alkylation damage to defend against alkylation-induced cell death or mutation. However, maintaining a proper balance of activity both within and between these pathways is crucial for an organism's favorable response to alkylating agents. Furthermore, an individual's response to alkylating agents can vary considerably from tissue to tissue and from person to person, pointing to genetic and epigenetic mechanisms that modulate alkylating agent toxicity. PMID:22237395

Immune tolerance: what is unique about the liver.

PubMed

Tiegs, Gisa; Lohse, Ansgar W

2010-02-01

The 'liver tolerance effect' mediates local and systemic tolerance to self and foreign antigens and has been attributed to specialized resident cells expressing anti-inflammatory mediators and inhibitory cell surface ligands for T cell activation. Non-parenchymal liver cells responsible for the tolerogenic properties of the liver are the resident dendritic cells (DCs), which comprise myeloid as well as plasmacytoid DCs, liver sinusoidal endothelial cells (LSECs), Kupffer cells (KCs) as well as hepatic stellate cells (HSCs), also known as Ito cells. These cells mediate immunosuppression by production of anti-inflammatory cytokines such as IL-10 and TGFbeta as well as by expression of the negative co-stimulator for T cell activation programmed cell death ligand-1 (PD-L1). An interesting observation in this context is that knockout of IL-10 or PD-L1 (or the receptor PD-1) does not necessarily result in inflammatory liver damage whereas transgenic inhibition of TGFbeta signaling induces liver disease in mice resembling chronic cholangitis. However, depending on the mouse model and on the type of injury, e.g. autoimmune disease, allograft rejection or viral infection, IL-10 or TGFbeta and/or PD-1 as well as cytotoxic T lymphocyte antigen-4 (CTLA-4) contribute to the immunosuppressive mechanisms of CD4(+)CD25(+)Foxp3(+) regulatory T cells (Tregs), which seem to be converted in the liver from infiltrating conventional naïve CD4(+) T cells and/or effector CD4(+) T cells to control the disease. Finally, hepatocytes also contribute to the 'liver tolerance effect' by expression of MHC class II molecules, probably low levels of co-stimulatory molecules and high levels of the co-inhibitory molecule PD-L1. Copyright 2009 Elsevier Ltd. All rights reserved.

DNA damage tolerance pathway involving DNA polymerase ι and the tumor suppressor p53 regulates DNA replication fork progression.

PubMed

Hampp, Stephanie; Kiessling, Tina; Buechle, Kerstin; Mansilla, Sabrina F; Thomale, Jürgen; Rall, Melanie; Ahn, Jinwoo; Pospiech, Helmut; Gottifredi, Vanesa; Wiesmüller, Lisa

2016-07-26

DNA damage tolerance facilitates the progression of replication forks that have encountered obstacles on the template strands. It involves either translesion DNA synthesis initiated by proliferating cell nuclear antigen monoubiquitination or less well-characterized fork reversal and template switch mechanisms. Herein, we characterize a novel tolerance pathway requiring the tumor suppressor p53, the translesion polymerase ι (POLι), the ubiquitin ligase Rad5-related helicase-like transcription factor (HLTF), and the SWI/SNF catalytic subunit (SNF2) translocase zinc finger ran-binding domain containing 3 (ZRANB3). This novel p53 activity is lost in the exonuclease-deficient but transcriptionally active p53(H115N) mutant. Wild-type p53, but not p53(H115N), associates with POLι in vivo. Strikingly, the concerted action of p53 and POLι decelerates nascent DNA elongation and promotes HLTF/ZRANB3-dependent recombination during unperturbed DNA replication. Particularly after cross-linker-induced replication stress, p53 and POLι also act together to promote meiotic recombination enzyme 11 (MRE11)-dependent accumulation of (phospho-)replication protein A (RPA)-coated ssDNA. These results implicate a direct role of p53 in the processing of replication forks encountering obstacles on the template strand. Our findings define an unprecedented function of p53 and POLι in the DNA damage response to endogenous or exogenous replication stress.

Damage-Tolerant Fan Casings for Jet Engines

NASA Technical Reports Server (NTRS)

2006-01-01

All turbofan engines work on the same principle. A large fan at the front of the engine draws air in. A portion of the air enters the compressor, but a greater portion passes on the outside of the engine this is called bypass air. The air that enters the compressor then passes through several stages of rotating fan blades that compress the air more, and then it passes into the combustor. In the combustor, fuel is injected into the airstream, and the fuel-air mixture is ignited. The hot gasses produced expand rapidly to the rear, and the engine reacts by moving forward. If there is a flaw in the system, such as an unexpected obstruction, the fan blade can break, spin off, and harm other engine components. Fan casings, therefore, need to be strong enough to contain errant blades and damage-tolerant to withstand the punishment of a loose blade-turned-projectile. NASA has spearheaded research into improving jet engine fan casings, ultimately discovering a cost-effective approach to manufacturing damage-tolerant fan cases that also boast significant weight reduction. In an aircraft, weight reduction translates directly into fuel burn savings, increased payload, and greater aircraft range. This technology increases safety and structural integrity; is an attractive, viable option for engine manufacturers, because of the low-cost manufacturing; and it is a practical alternative for customers, as it has the added cost saving benefits of the weight reduction.

Silicon Priming Created an Enhanced Tolerance in Alfalfa (Medicago sativa L.) Seedlings in Response to High Alkaline Stress.

PubMed

Liu, Duo; Liu, Miao; Liu, Xiao-Long; Cheng, Xian-Guo; Liang, Zheng-Wei

2018-01-01

Alkaline stress as a result of higher pH usually triggers more severe physiological damage to plants than that of saline stress with a neutral pH. In the present study, we demonstrated that silicon (Si) priming of alfalfa ( Medicago sativa L.) seedlings increased their tolerance to high alkaline stress situations. Gongnong No. 1 seedlings were subjected to alkaline stress simulated by 25 mM Na 2 CO 3 (pH 11.2). Alkaline stress greatly decreased the biomass and caused severe lodging or wilting of alfalfa seedlings. In contrast, the application of Si to alfalfa seedlings 36 h prior to the alkaline treatment significantly alleviated the damage symptoms and greatly increased the biomass and chlorophyll content. Because of being concomitant with increasing photosynthesis and water use efficiency, decreasing membrane injury and malondialdehyde content, and increasing peroxidase and catalase ascorbate activities in alfalfa leaves, thereby alleviating the triggered oxidative damage by alkaline stress to the plant. Furthermore, Si priming significantly decreased the accumulation of protein and proline content in alfalfa, thus reducing photosynthetic feedback repression. Si priming significantly accumulated more Na in the roots, but led to a decrease of Na accumulation and an increase of K accumulation in the leaves under alkaline stress. Meanwhile, Si priming decreased the accumulation of metal ions such as Mg, Fe, Mn, and Zn in the roots of alfalfa seedlings under alkaline stress. Collectively, these results suggested that Si is involved in the metabolic or physiological changes and has a potent priming effect on the alkaline tolerance of alfalfa seedlings. The present study indicated that Si priming is a new approach to improve the alkaline tolerance in alfalfa and provides increasing information for further exploration of the alkaline stress response at the molecular level in alfalfa.

Increase in Furfural Tolerance in Ethanologenic Escherichia coli LY180 by Plasmid-Based Expression of thyA

PubMed Central

Zheng, Huabao; Wang, Xuan; Yomano, Lorraine P.; Shanmugam, Keelnatham T.

2012-01-01

Furfural is an inhibitory side product formed during the depolymerization of hemicellulose by mineral acids. Genomic libraries from three different bacteria (Bacillus subtilis YB886, Escherichia coli NC3, and Zymomonas mobilis CP4) were screened for genes that conferred furfural resistance on plates. Beneficial plasmids containing the thyA gene (coding for thymidylate synthase) were recovered from all three organisms. Expression of this key gene in the de novo pathway for dTMP biosynthesis improved furfural resistance on plates and during fermentation. A similar benefit was observed by supplementation with thymine, thymidine, or the combination of tetrahydrofolate and serine (precursors for 5,10-methylenetetrahydrofolate, the methyl donor for ThyA). Supplementation with deoxyuridine provided a small benefit, and deoxyribose was of no benefit for furfural tolerance. A combination of thymidine and plasmid expression of thyA was no more effective than either alone. Together, these results demonstrate that furfural tolerance is increased by approaches that increase the supply of pyrimidine deoxyribonucleotides. However, ThyA activity was not directly affected by the addition of furfural. Furfural has been previously shown to damage DNA in E. coli and to activate a cellular response to oxidative damage in yeast. The added burden of repairing furfural-damaged DNA in E. coli would be expected to increase the cellular requirement for dTMP. Increased expression of thyA (E. coli, B. subtilis, or Z. mobilis), supplementation of cultures with thymidine, and supplementation with precursors for 5,10-methylenetetrahydrofolate (methyl donor) are each proposed to increase furfural tolerance by increasing the availability of dTMP for DNA repair. PMID:22504824

Increase in furfural tolerance in ethanologenic Escherichia coli LY180 by plasmid-based expression of thyA.

PubMed

Zheng, Huabao; Wang, Xuan; Yomano, Lorraine P; Shanmugam, Keelnatham T; Ingram, Lonnie O

2012-06-01

Furfural is an inhibitory side product formed during the depolymerization of hemicellulose by mineral acids. Genomic libraries from three different bacteria (Bacillus subtilis YB886, Escherichia coli NC3, and Zymomonas mobilis CP4) were screened for genes that conferred furfural resistance on plates. Beneficial plasmids containing the thyA gene (coding for thymidylate synthase) were recovered from all three organisms. Expression of this key gene in the de novo pathway for dTMP biosynthesis improved furfural resistance on plates and during fermentation. A similar benefit was observed by supplementation with thymine, thymidine, or the combination of tetrahydrofolate and serine (precursors for 5,10-methylenetetrahydrofolate, the methyl donor for ThyA). Supplementation with deoxyuridine provided a small benefit, and deoxyribose was of no benefit for furfural tolerance. A combination of thymidine and plasmid expression of thyA was no more effective than either alone. Together, these results demonstrate that furfural tolerance is increased by approaches that increase the supply of pyrimidine deoxyribonucleotides. However, ThyA activity was not directly affected by the addition of furfural. Furfural has been previously shown to damage DNA in E. coli and to activate a cellular response to oxidative damage in yeast. The added burden of repairing furfural-damaged DNA in E. coli would be expected to increase the cellular requirement for dTMP. Increased expression of thyA (E. coli, B. subtilis, or Z. mobilis), supplementation of cultures with thymidine, and supplementation with precursors for 5,10-methylenetetrahydrofolate (methyl donor) are each proposed to increase furfural tolerance by increasing the availability of dTMP for DNA repair.

Advanced Information Processing System - Fault detection and error handling

NASA Technical Reports Server (NTRS)

Lala, J. H.

1985-01-01

The Advanced Information Processing System (AIPS) is designed to provide a fault tolerant and damage tolerant data processing architecture for a broad range of aerospace vehicles, including tactical and transport aircraft, and manned and autonomous spacecraft. A proof-of-concept (POC) system is now in the detailed design and fabrication phase. This paper gives an overview of a preliminary fault detection and error handling philosophy in AIPS.

Overexpression of a bacterial mercury transporter MerT in Arabidopsis enhances mercury tolerance.

PubMed

Xu, Sheng; Sun, Bin; Wang, Rong; He, Jia; Xia, Bing; Xue, Yong; Wang, Ren

2017-08-19

The phytoremediation by using of green plants in the removal of environmental pollutant is an environment friendly, green technology that is cost effective and energetically inexpensive. By using Agrobacterium-mediated gene transfer, we generated transgenic Arabidopsis plants ectopically expressing mercuric transport protein gene (merT) from Pseudomonas alcaligenes. Compared with wild-type (WT) plants, overexpressing PamerT in Arabidopsis enhanced the tolerance to HgCl 2 . Further results showed that the enhanced total activities or corresponding transcripts of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT) and guaiacol peroxidase (POD) were observed in transgenic Arabidopsis under HgCl 2 stress. These results were confirmed by the alleviation of oxidative damage, as indicated by the decrease of thiobarbituric acid reactive substances (TBARS) contents and reactive oxygen species (ROS) accumulation. In addition, localization analysis of PaMerT in Arabidopsis protoplast showed that it is likely to be associated with vacuole. In all, PamerT increased mercury (Hg) tolerance in transgenic Arabidopsis, and decreased production of Hg-induced ROS, thereby protecting plants from oxidative damage. The present study has provided further evidence that bacterial MerT plays an important role in the plant tolerance to HgCl 2 and in reducing the production of ROS induced by HgCl 2 . Copyright © 2017 Elsevier Inc. All rights reserved.

Analyses of flooding tolerance of soybean varieties at emergence and varietal differences in their proteomes.

PubMed

Nanjo, Yohei; Jang, Hee-Young; Kim, Hong-Sig; Hiraga, Susumu; Woo, Sun-Hee; Komatsu, Setsuko

2014-10-01

Flooding of fields due to heavy and/or continuous rainfall influences soybean production. To identify soybean varieties with flooding tolerance at the seedling emergence stage, 128 soybean varieties were evaluated using a flooding tolerance index, which is based on plant survival rates, the lack of apparent damage and lateral root development, and post-flooding radicle elongation rate. The soybean varieties were ranked according to their flooding tolerance index, and it was found that the tolerance levels of soybean varieties exhibit a continuum of differences between varieties. Subsequently, tolerant, moderately tolerant and sensitive varieties were selected and subjected to comparative proteomic analysis to clarify the tolerance mechanism. Proteomic analysis of the radicles, combined with correlation analysis, showed that the ratios of RNA binding/processing related proteins and flooding stress indicator proteins were significantly correlated with flooding tolerance index. The RNA binding/processing related proteins were positively correlated in untreated soybeans, whereas flooding stress indicator proteins were negatively correlated in flooded soybeans. These results suggest that flooding tolerance is regulated by mechanisms through multiple factors and is associated with abundance levels of the identified proteins. Copyright © 2014 Elsevier Ltd. All rights reserved.

Exogenous Proline and Glycine Betaine Mediated Upregulation of Antioxidant Defense and Glyoxalase Systems Provides Better Protection against Salt-Induced Oxidative Stress in Two Rice (Oryza sativa L.) Varieties

PubMed Central

Hasanuzzaman, Mirza; Alam, Md. Mahabub; Rahman, Anisur; Hasanuzzaman, Md.; Nahar, Kamrun; Fujita, Masayuki

2014-01-01

The present study investigates the roles of exogenous proline (Pro, 5 mM) and glycine betaine (GB, 5 mM) in improving salt stress tolerance in salt sensitive (BRRI dhan49) and salt tolerant (BRRI dhan54) rice (Oryza sativa L.) varieties. Salt stresses (150 and 300 mM NaCl for 48 h) significantly reduced leaf relative water (RWC) and chlorophyll (chl) content and increased endogenous Pro and increased lipid peroxidation and H2O2 levels. Ascorbate (AsA), glutathione (GSH) and GSH/GSSG, ascorbate peroxidae (APX), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), glutathione reductase (GR), glutathione peroxidase (GPX), catalase (CAT), and glyoxalase I (Gly I) activities were reduced in sensitive variety and these were increased in tolerant variety due to salt stress. The glyoxalase II (Gly II), glutathione S-transferase (GST), and superoxide dismutase (SOD) activities were increased in both cultivars by salt stress. Exogenous Pro and GB application with salt stress improved physiological parameters and reduced oxidative damage in both cultivars where BRRI dhan54 showed better tolerance. The result suggests that exogenous application of Pro and GB increased rice seedlings' tolerance to salt-induced oxidative damage by upregulating their antioxidant defense system where these protectants rendered better performance to BRRI dhan54 and Pro can be considered as better protectant than GB. PMID:24991566

Analysis of DNA repair and protection in the Tardigrade Ramazzottius varieornatus and Hypsibius dujardini after exposure to UVC radiation.

PubMed

Horikawa, Daiki D; Cumbers, John; Sakakibara, Iori; Rogoff, Dana; Leuko, Stefan; Harnoto, Raechel; Arakawa, Kazuharu; Katayama, Toshiaki; Kunieda, Takekazu; Toyoda, Atsushi; Fujiyama, Asao; Rothschild, Lynn J

2013-01-01

Tardigrades inhabiting terrestrial environments exhibit extraordinary resistance to ionizing radiation and UV radiation although little is known about the mechanisms underlying the resistance. We found that the terrestrial tardigrade Ramazzottius varieornatus is able to tolerate massive doses of UVC irradiation by both being protected from forming UVC-induced thymine dimers in DNA in a desiccated, anhydrobiotic state as well as repairing the dimers that do form in the hydrated animals. In R. varieornatus accumulation of thymine dimers in DNA induced by irradiation with 2.5 kJ/m(2) of UVC radiation disappeared 18 h after the exposure when the animals were exposed to fluorescent light but not in the dark. Much higher UV radiation tolerance was observed in desiccated anhydrobiotic R. varieornatus compared to hydrated specimens of this species. On the other hand, the freshwater tardigrade species Hypsibius dujardini that was used as control, showed much weaker tolerance to UVC radiation than R. varieornatus, and it did not contain a putative phrA gene sequence. The anhydrobiotes of R. varieornatus accumulated much less UVC-induced thymine dimers in DNA than hydrated one. It suggests that anhydrobiosis efficiently avoids DNA damage accumulation in R. varieornatus and confers better UV radiation tolerance on this species. Thus we propose that UV radiation tolerance in tardigrades is due to the both high capacities of DNA damage repair and DNA protection, a two-pronged survival strategy.

Analysis of DNA Repair and Protection in the Tardigrade Ramazzottius varieornatus and Hypsibius dujardini after Exposure to UVC Radiation

PubMed Central

Horikawa, Daiki D.; Cumbers, John; Sakakibara, Iori; Rogoff, Dana; Leuko, Stefan; Harnoto, Raechel; Arakawa, Kazuharu; Katayama, Toshiaki; Kunieda, Takekazu; Toyoda, Atsushi; Fujiyama, Asao; Rothschild, Lynn J.

2013-01-01

Tardigrades inhabiting terrestrial environments exhibit extraordinary resistance to ionizing radiation and UV radiation although little is known about the mechanisms underlying the resistance. We found that the terrestrial tardigrade Ramazzottius varieornatus is able to tolerate massive doses of UVC irradiation by both being protected from forming UVC-induced thymine dimers in DNA in a desiccated, anhydrobiotic state as well as repairing the dimers that do form in the hydrated animals. In R. varieornatus accumulation of thymine dimers in DNA induced by irradiation with 2.5 kJ/m2 of UVC radiation disappeared 18 h after the exposure when the animals were exposed to fluorescent light but not in the dark. Much higher UV radiation tolerance was observed in desiccated anhydrobiotic R. varieornatus compared to hydrated specimens of this species. On the other hand, the freshwater tardigrade species Hypsibius dujardini that was used as control, showed much weaker tolerance to UVC radiation than R. varieornatus, and it did not contain a putative phrA gene sequence. The anhydrobiotes of R. varieornatus accumulated much less UVC-induced thymine dimers in DNA than hydrated one. It suggests that anhydrobiosis efficiently avoids DNA damage accumulation in R. varieornatus and confers better UV radiation tolerance on this species. Thus we propose that UV radiation tolerance in tardigrades is due to the both high capacities of DNA damage repair and DNA protection, a two-pronged survival strategy. PMID:23762256

Roles of Plasmalemma Aquaporin Gene StPIP1 in Enhancing Drought Tolerance in Potato

PubMed Central

Wang, Li; Liu, Yuhui; Feng, Shoujiang; Yang, Jiangwei; Li, Dan; Zhang, Junlian

2017-01-01

Survival and mortality of plants in response to severe drought may be related to carbon starvation, but little is known about how plasma membrane intrinsic proteins may help alleviate the drought-induced damage. Here, we determined the roles of plasmalemma aquaporin gene in improving plant water status, maintaining carbon accumulation, and thereby enhancing drought tolerance. Seven StPIP1 transformed potato (Solanum tuberosum L.) lines (namely T1, T2…T7) were compared with non-transgenic control plant at molecule and whole-plant levels. The relative expression of StPIP1 gene was found in leaves, stems and roots, with the most abundant expression being in the roots. The transgenic lines T6 and T7 had the highest StPIP1 expression, averaging 7.2 times that of the control and the greatest differences occurred 48 h after mannitol osmotic stress treatment. Using an evaluation index to quantifying the degree of drought tolerance, we found that the StPIP1 transgenic lines T6 and T7 had the highest drought tolerance, averaging 8.5 times that of the control. Measured at 30 days in drought stress treatment, the control plant decreased net photosynthetic rate by 33 and 56%, respectively, under moderate and severe stresses; also decreased stomatal conductance by 39 and 65%; and lowered transpiration rate by 49 and 69%, compared to the no-stress treatment, whereas the transgenic lines T6 and T7 maintained a relatively stable level with slight decreases in these properties. The constitutive overexpression of StPIP1 in potato improved plant water use efficiency and increased nonstructural carbohydrate concentration, which helped alleviate carbon starvation and minimized the loss of biomass and tuber yield due to drought stress. We conclude that the expression of StPIPs improves overall water relations in the plant and helps maintain photosynthesis and stomatal conductance; these help minimize carbon starvation and enhance the whole plant tolerance to drought stress. PMID:28487712

77 FR 56172 - Airworthiness Directives; Airbus Airplanes

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-12

... high tensile standing stress, resulting from dry fit axle assembly method. Improvement has been introduced subsequently with a grease fit axle assembly method. Fatigue and damage tolerance analyses were...

14 CFR 67.107 - Mental.

Code of Federal Regulations, 2010 CFR

2010-01-01

...) beverages, as evidenced by— (A) Increased tolerance; (B) Manifestation of withdrawal symptoms; (C) Impaired control of use; or (D) Continued use despite damage to physical health or impairment of social, personal...

14 CFR 67.307 - Mental.

Code of Federal Regulations, 2010 CFR

2010-01-01

...) beverages, as evidenced by— (A) Increased tolerance; (B) Manifestation of withdrawal symptoms; (C) Impaired control of use; or (D) Continued use despite damage to physical health or impairment of social, personal...

14 CFR 67.207 - Mental.

Code of Federal Regulations, 2010 CFR

2010-01-01

...) beverages, as evidenced by— (A) Increased tolerance; (B) Manifestation of withdrawal symptoms; (C) Impaired control of use; or (D) Continued use despite damage to physical health or impairment of social, personal...

DOE Office of Scientific and Technical Information (OSTI.GOV)

Li, Jin; Fan, Cuncai; Ding, Jie

High energy particle radiations induce severe microstructural damage in metallic materials. Nanoporous materials with a giant surface-to-volume ratio may alleviate radiation damage in irradiated metallic materials as free surface are defect sinks. We show, by using in situ Kr ion irradiation in a transmission electron microscope at room temperature, that nanoporous Au indeed has significantly improved radiation tolerance comparing with coarse-grained, fully dense Au. In situ studies show that nanopores can absorb and eliminate a large number of radiation-induced defect clusters. Meanwhile, nanopores shrink (self-heal) during radiation, and their shrinkage rate is pore size dependent. Furthermore, the in situ studiesmore » show dose-rate-dependent diffusivity of defect clusters. Our study sheds light on the design of radiation-tolerant nanoporous metallic materials for advanced nuclear reactor applications.« less

Multi-Functional Sandwich Composites for Spacecraft Applications: An Initial Assessment

NASA Technical Reports Server (NTRS)

Adams, Daniel O.; Webb, Nicholas Jason; Yarger, Cody B.; Hunter, Abigail; Oborn, Kelli D.

2007-01-01

Current spacecraft implement relatively uncoupled material and structural systems to address a variety of design requirements, including structural integrity, damage tolerance, radiation protection, debris shielding and thermal insulation. This investigation provided an initial assessment of multi-functional sandwich composites to integrate these diverse requirements. The need for radiation shielding was addressed through the selection of polymeric constituents with high hydrogen content. To provide increased damage tolerance and debris shielding, manufacturing techniques were developed to incorporate transverse stitching reinforcement, internal layers, and a self-healing ionomer membrane. To assess the effects of a space environment, thermal expansion behavior of the candidate foam materials was investigated under a vacuum and increasing temperature. Finally, a thermal expansion model was developed for foam under vacuum conditions and its predictive capability assessed.

Magmas functions as a ROS regulator and provides cytoprotection against oxidative stress-mediated damages

PubMed Central

Srivastava, S; Sinha, D; Saha, P P; Marthala, H; D'Silva, P

2014-01-01

Redox imbalance generates multiple cellular damages leading to oxidative stress-mediated pathological conditions such as neurodegenerative diseases and cancer progression. Therefore, maintenance of reactive oxygen species (ROS) homeostasis is most important that involves well-defined antioxidant machinery. In the present study, we have identified for the first time a component of mammalian protein translocation machinery Magmas to perform a critical ROS regulatory function. Magmas overexpression has been reported in highly metabolically active tissues and cancer cells that are prone to oxidative damage. We found that Magmas regulates cellular ROS levels by controlling its production as well as scavenging. Magmas promotes cellular tolerance toward oxidative stress by enhancing antioxidant enzyme activity, thus preventing induction of apoptosis and damage to cellular components. Magmas enhances the activity of electron transport chain (ETC) complexes, causing reduced ROS production. Our results suggest that J-like domain of Magmas is essential for maintenance of redox balance. The function of Magmas as a ROS sensor was found to be independent of its role in protein import. The unique ROS modulatory role of Magmas is highlighted by its ability to increase cell tolerance to oxidative stress even in yeast model organism. The cytoprotective capability of Magmas against oxidative damage makes it an important candidate for future investigation in therapeutics of oxidative stress-related diseases. PMID:25165880

Assignments of the Raman modes of monoclinic erbium oxide

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yan, D.; Wu, P., E-mail: pingwu@sas.ustb.edu.cn; Zhang, S. P.

2013-11-21

As a heavy rare earth oxide, erbium oxide (Er{sub 2}O{sub 3}) has many attractive properties. Monoclinic Er{sub 2}O{sub 3} has useful properties not found in stable cubic Er{sub 2}O{sub 3}, such as unique optical properties and high radiation damage tolerance. In this study, cubic Er{sub 2}O{sub 3} coating and Er{sub 2}O{sub 3} coating with mixed phases were prepared. The Raman scattering spectra of these coatings were investigated by using a confocal micro-Raman spectrometer equipped with 325, 473, 514, 532, 633, and 784 nm lasers. A total of 17 first-order Raman modes of monoclinic Er{sub 2}O{sub 3} were identified and assigned. Themore » modes at 83, 112, 152, 170, 278, 290, 409, 446, 478, 521, 603, and 622 cm{sup −1} are of A{sub g} symmetry, whereas modes at 71, 98, 333, 409, 446, and 468 cm{sup −1} are of B{sub g} symmetry. This research provides basic data necessary for the characterization of monoclinic Er{sub 2}O{sub 3} by Raman spectroscopy.« less

Attenuated microglial activation mediates tolerance to the neurotoxic effects of methamphetamine.

PubMed

Thomas, David M; Kuhn, Donald M

2005-02-01

Methamphetamine causes persistent damage to dopamine nerve endings of the striatum. Repeated, intermittent treatment of mice with low doses of methamphetamine leads to the development of tolerance to its neurotoxic effects. The mechanisms underlying tolerance are not understood but clearly involve more than alterations in drug bioavailability or reductions in the hyperthermia caused by methamphetamine. Microglia have been implicated recently as mediators of methamphetamine-induced neurotoxicity. The purpose of the present studies was to determine if a tolerance regimen of methamphetamine would attenuate the microglial response to a neurotoxic challenge. Mice treated with a low-dose methamphetamine tolerance regimen showed minor reductions in striatal dopamine content and low levels of microglial activation. When the tolerance regimen preceded a neurotoxic challenge of methamphetamine, the depletion of dopamine normally seen was significantly attenuated. The microglial activation that occurs after a toxic methamphetamine challenge was blunted likewise. Despite the induction of tolerance against drug-induced toxicity and microglial activation, a neurotoxic challenge with methamphetamine still caused hyperthermia. These results suggest that tolerance to methamphetamine neurotoxicity is associated with attenuated microglial activation and they further dissociate its neurotoxicity from drug-induced hyperthermia.

Recent and Future Enhancements in NDI for Aircraft Structures (Postprint)

DTIC Science & Technology

2015-11-01

found that different capabilities were being used to determine inspection intervals for different aircraft [7]. This led to an internal effort...capability of the NDI technique determines the inspection intervals and the Distribution Statement A. Approved for public release; distribution...damage and that the aircraft structure had to be inspectable . The results of the damage tolerance assessments were incorporated into USAF Technical

Recent and Future Enhancement in NDI for Aircraft Structures (Postprint)

DTIC Science & Technology

2015-11-01

found that different capabilities were being used to determine inspection intervals for different aircraft [7]. This led to an internal effort...capability of the NDI technique determines the inspection intervals and the Distribution Statement A. Approved for public release; distribution...damage and that the aircraft structure had to be inspectable . The results of the damage tolerance assessments were incorporated into USAF Technical

Structural Integrity and Aging-Related Issues of Helicopters

DTIC Science & Technology

2000-10-01

inherently damage lolerant , any damage- inspection in critical locations where tests have indicated tolerant features in airframe design only enhances...required, so European Rotorcraft Forum. Marseilles, France, 15- that helicopters are equipped with such features as fly- 17 September 1998 . by-wire and...fatigue Evaluation of structural integrity issues of aging helicopters. The Structure," 29 April, 1998 . extended safe-life approach encompasses the best

Pro-oxidant Induced DNA Damage in Human Lymphoblastoid Cells: Homeostatic Mechanisms of Genotoxic Tolerance

PubMed Central

Seager, Anna L.

2012-01-01

Oxidative stress contributes to many disease etiologies including ageing, neurodegeneration, and cancer, partly through DNA damage induction (genotoxicity). Understanding the i nteractions of free radicals with DNA is fundamental to discern mutation risks. In genetic toxicology, regulatory authorities consider that most genotoxins exhibit a linear relationship between dose and mutagenic response. Yet, homeostatic mechanisms, including DNA repair, that allow cells to tolerate low levels of genotoxic exposure exist. Acceptance of thresholds for genotoxicity has widespread consequences in terms of understanding cancer risk and regulating human exposure to chemicals/drugs. Three pro-oxidant chemicals, hydrogen peroxide (H2O2), potassium bromate (KBrO3), and menadione, were examined for low dose-response curves in human lymphoblastoid cells. DNA repair and antioxidant capacity were assessed as possible threshold mechanisms. H2O2 and KBrO3, but not menadione, exhibited thresholded responses, containing a range of nongenotoxic low doses. Levels of the DNA glycosylase 8-oxoguanine glycosylase were unchanged in response to pro- oxidant stress. DNA repair–focused gene expression arrays reported changes in ATM and BRCA1, involved in double-strand break repair, in response to low-dose pro-oxidant exposure; however, these alterations were not substantiated at the protein level. Determination of oxidatively induced DNA damage in H2O2-treated AHH-1 cells reported accumulation of thymine glycol above the genotoxic threshold. Further, the H2O2 dose-response curve was shifted by modulating the antioxidant glutathione. Hence, observed pro- oxidant thresholds were due to protective capacities of base excision repair enzymes and antioxidants against DNA damage, highlighting the importance of homeostatic mechanisms in “genotoxic tolerance.” PMID:22539617

The Significance of Small Cracks in Fatigue Design Concepts as Related to Rotorcraft Metallic Dynamic Components

NASA Technical Reports Server (NTRS)

Everett, R. A., Jr.; Elber, W.

2000-01-01

In this paper the significance of the "small" crack effect as defined in fracture mechanics will be discussed as it relates to life managing rotorcraft dynamic components using the conventional safe-life, the flaw tolerant safe-life, and the damage tolerance design philosophies. These topics will be introduced starting with an explanation of the small-crack theory, then showing how small-crack theory has been used to predict the total fatigue life of fatigue laboratory test coupons with and without flaws, and concluding with how small cracks can affect the crack-growth damage tolerance design philosophy. As stated in this paper the "small" crack effect is defined in fracture mechanics where it has been observed that cracks on the order of 300 microns or less in length will propagate at higher growth rates than long cracks and also will grow at AK values below the long crack AK threshold. The small-crack effect is illustrated herein as resulting from a lack of crack closure and is explained based on continuum mechanics principles using crack-closure concepts in fracture mechanics.

Damage assessment in multilayered MEMS structures under thermal fatigue

NASA Astrophysics Data System (ADS)

Maligno, A. R.; Whalley, D. C.; Silberschmidt, V. V.

2011-07-01

This paper reports on the application of a Physics of Failure (PoF) methodology to assessing the reliability of a micro electro mechanical system (MEMS). Numerical simulations, based on the finite element method (FEM) using a sub-domain approach was used to examine the damage onset due to temperature variations (e.g. yielding of metals which may lead to thermal fatigue). In this work remeshing techniques were employed in order to develop a damage tolerance approach based on the assumption that initial flaws exist in the multi-layered.

Progressive Damage and Fracture of Unstiffened and Stiffened Composite Pressure Vessels

NASA Technical Reports Server (NTRS)

Minnetyan, Levon; Gotsis, Pascal K.; Chamis, Christos C.

1997-01-01

Structural durability and damage tolerance characteristics of pressurized graphite/epoxy laminated thin composite cylinders are investigated via computational simulation. Both unstiffened and integral hoop stiffened cylinders are considered. A computer code is utilized for the simulation of composite structural degradation under loading. Damage initiation, growth, accumulation, and propagation to structural fracture are included in the simulation. The increase of burst pressure due to hoop stiffening is quantified. Results demonstrate the significance of the type and size of local defects on the structural durability of pressurized composite cylindrical shells.

Shaped fiber composites

DOEpatents

Kinnan, Mark K.; Roach, Dennis P.

2017-12-05

A composite article is disclosed that has non-circular fibers embedded in a polymer matrix. The composite article has improved damage tolerance, toughness, bending, and impact resistance compared to composites having traditional round fibers.

ACEE composite structures technology

NASA Technical Reports Server (NTRS)

James, A. M.

1984-01-01

Topics addressed include: strength and hygrothermal response of L-1011 fin components; wing fuel containment and damage tolerance development; impact dynamics; acoustic transmission; fuselage structure; composite transport wing technology development; spar/assembly concepts.

7 CFR 993.601 - More restrictive grade regulation.

Code of Federal Regulations, 2010 CFR

2010-01-01

... condition, end cracks, fermentation, skin or flesh damage, scab, burned, mold, imbedded dirt, insect... value. (2) The combined tolerance allowance for mold, brown rot, imbedded dirt, insect infestation, and...

Engineering of global regulators and cell surface properties toward enhancing stress tolerance in Saccharomyces cerevisiae.

PubMed

Kuroda, Kouichi; Ueda, Mitsuyoshi

2017-12-01

Microbial cell factories are subject to various stresses, leading to the reductions of metabolic activity and bioproduction efficiency. Therefore, the development of stress-tolerant microorganisms is important for improving bio-production efficiency. Recently, modifications of cell surface properties and master regulators have been shown to be effective approaches for enhancing stress tolerance. The cell surface is an attractive target owing to its interactions with the environment and its role in transmitting environmental information. Cell surface engineering in yeast has enabled the convenient modification of cell surface properties. Displaying random peptide libraries and subsequent screening can successfully improve stress tolerance. Furthermore, master regulators including transcription factors are also promising target to be engineered because stress tolerance is determined by many cooperative factors and modification of master regulators can simultaneously affect the expression of multiple downstream genes. The key single amino acid mutations in transcription factors have been identified by analyzing tolerant yeasts that were isolated by adaptive evolution under stress conditions. This enabled the reconstruction of stress-tolerant yeast without burdening cells by introducing the identified mutations. Therefore, for the construction of stress-tolerant yeast from any strains, these two approaches are promising alternatives to conventional overexpression and deletion of stress-related genes. Copyright © 2017 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

Hardness properties and microscopic investigation of crack- crystal interaction in SiO(2)-MgO-Al(2)O(3)-K(2)O-B(2)O(3)-F glass ceramic system.

PubMed

Roy, Shibayan; Basu, Bikramjit

2010-01-01

In view of the potential engineering applications requiring machinability and wear resistance, the present work focuses to evaluate hardness property and to understand the damage behavior of some selected glass-ceramics having different crystal morphologies with SiO(2)-MgO-Al(2)O(3)-K(2)O-B(2)O(3)-F composition, using static micro-indentation tests as well as dynamic scratch tests, respectively. Vickers hardness of up to 5.5 GPa has been measured in glass-ceramics containing plate like mica crystals. Scratch tests at a high load of 50 Nin artificial saliva were carried out in order to simulate the crack-microstructure interaction during real-time abrasion wear and machining operation. The experimental observations indicate that the novel "spherulitic-dendritic shaped "crystals, similar to the plate like crystals, have the potential to hinder the scratching induced crack propagation. In particular, such potential of the 'spherulitic-dendritic' crystals become more effective due to the larger interfacial area with the glass matrix as well as the dendritic structure of each mica plate, which helps in crack deflection and crack blunting, to a larger extent.While modest damage tolerant behavior is observed in case of 'spherulitic-dendritic' crystal containing material, severe brittle fracture of plate like crystals were noted, when both were scratched at 50 N load.

How endangered is sexual reproduction of high-mountain plants by summer frosts? Frost resistance, frequency of frost events and risk assessment.

PubMed

Ladinig, Ursula; Hacker, Jürgen; Neuner, Gilbert; Wagner, Johanna

2013-03-01

In temperate-zone mountains, summer frosts usually occur during unpredictable cold spells with snow-falls. Earlier studies have shown that vegetative aboveground organs of most high-mountain plants tolerate extracellular ice in the active state. However, little is known about the impact of frost on reproductive development and reproductive success. In common plant species from the European Alps (Cerastium uniflorum, Loiseleuria procumbens, Ranunculus glacialis, Rhododendron ferrugineum, Saxifraga bryoides, S. moschata, S. caesia), differing in growth form, altitudinal distribution and phenology, frost resistance of reproductive and vegetative shoots was assessed in different reproductive stages. Intact plants were exposed to simulated night frosts between -2 and -14 °C in temperature-controlled freezers. Nucleation temperatures, freezing damage and subsequent reproductive success (fruit and seed set, seed germination) were determined. During all reproductive stages, reproductive shoots were significantly less frost resistant than vegetative shoots (mean difference for LT50 -4.2 ± 2.7 K). In most species, reproductive shoots were ice tolerant before bolting and during fruiting (mean LT50 -7 and -5.7 °C), but were ice sensitive during bolting and anthesis (mean LT50 around -4 °C). Only R. glacialis remained ice tolerant during all reproductive stages. Frost injury in reproductive shoots usually led to full fruit loss. Reproductive success of frost-treated but undamaged shoots did not differ significantly from control values. Assessing the frost damage risk on the basis of summer frost frequency and frost resistance shows that, in the alpine zone, low-statured species are rarely endangered as long as they are protected by snow. The situation is different in the subnival and nival zone, where frost-sensitive reproductive shoots may become frost damaged even when covered by snow. Unprotected individuals are at high risk of suffering from frost damage, particularly at higher elevations. It appears that ice tolerance in reproductive structures is an advantage but not an absolute precondition for colonizing high altitudes with frequent frost events.

Damage tolerance of bonded composite aircraft repairs for metallic structures

NASA Astrophysics Data System (ADS)

Clark, Randal John

This thesis describes the development and validation of methods for damage tolerance substantiation of bonded composite repairs applied to cracked plates. This technology is used to repair metal aircraft structures, offering improvements in fatigue life, cost, manufacturability, and inspectability when compared to riveted repairs. The work focuses on the effects of plate thickness and bending on repair life, and covers fundamental aspects of fracture and fatigue of cracked plates and bonded joints. This project falls under the UBC Bonded Composite Repair Program, which has the goal of certification and widespread use of bonded repairs in civilian air transportation. This thesis analyses the plate thickness and transverse stress effects on fracture of repaired plates and the related problem of induced geometrically nonlinear bending in unbalanced (single-sided) repairs. The author begins by developing a classification scheme for assigning repair damage tolerance substantiation requirements based upon stress-based adhesive fracture/fatigue criteria and the residual strength of the original structure. The governing equations for bending of cracked plates are then reformulated and line-spring models are developed for linear and nonlinear coupled bending and extension of reinforced cracks. The line-spring models were used to correct the Wang and Rose energy method for the determination of the long-crack limit stress intensity, and to develop a new interpolation model for repaired cracks of arbitrary length. The analysis was validated using finite element models and data from mechanical tests performed on hybrid bonded joints and repair specimens that are representative of an in-service repair. This work will allow designers to evaluate the damage tolerance of the repaired plate, the adhesive, and the composite patch, which is an airworthiness requirement under FAR (Federal Aviation Regulations) 25.571. The thesis concludes by assessing the remaining barriers to certification of bonded repairs, discussing the results of the analysis, and making suggestions for future work. The developed techniques should also prove to be useful for the analysis of fibre-reinforced metal laminates and other layered structures. Some concepts are general and should be useful in the analysis of any plate with large in-plane stress gradients that lead to significant transverse stresses.

Piroxicam-β-Cyclodextrin: A GI Safer Piroxicam

PubMed Central

Scarpignato, C

2013-01-01

Although NSAIDs are very effective drugs, their use is associated with a broad spectrum of adverse reactions in the liver, kidney, cardiovascular (CV) system, skin and gut. Gastrointestinal (GI) side effects are the most common and constitute a wide clinical spectrum ranging from dyspepsia, heartburn and abdominal discomfort to more serious events such as peptic ulcer with life-threatening complications of bleeding and perforation. The appreciation that CV risk is also increased further complicates the choices of physicians prescribing anti-inflammatory therapy. Despite prevention strategies should be implemented in patients at risk, gastroprotection is often underused and adherence to treatment is generally poor. A more appealing approach would be therefore to develop drugs that are devoid of or have reduced GI toxicity. Gastro-duodenal mucosa possesses many defensive mechanisms and NSAIDs have a deleterious effect on most of them. This results in a mucosa less able to cope with even a reduced acid load. NSAIDs cause gastro-duodenal damage, by two main mechanisms: a physiochemical disruption of the gastric mucosal barrier and systemic inhibition of gastric mucosal protection, through inhibition of cyclooxygenase (COX, PG endoperoxide G/H synthase) activity of the GI mucosa. However, against a background of COX inhibition by anti-inflammatory doses of NSAIDs, their physicochemical properties, in particular their acidity, underlie the topical effect leading to short-term damage. It has been shown that esterification of acidic NSAIDs suppresses their gastrotoxicity without adversely affecting anti-inflammatory activity. Another way to develop NSAIDs with better GI tolerability is to complex these molecules with cyclodextrins (CDs), giving rise to so-called “inclusion complexes” that can have physical, chemical and biological properties very different from either those of the drug or the cyclodextrin. Complexation of NSAIDs with β-cyclodextrin potentially leads to a more rapid onset of action after oral administration and improved GI tolerability because of minimization of the drug gastric effects. One such drug, piroxicam-β-cyclodextrin (PBC), has been used in Europe for 25 years. Preclinical and clinical pharmacology of PBC do show that the β-cyclodextrin inclusion complex of piroxicam is better tolerated from the upper GI tract than free piroxicam, while retaining all the analgesic and anti-inflammatory properties of the parent compound. In addition, the drug is endowed with a quick absorption rate, which translates into a faster onset of analgesic activity, an effect confirmed in several clinical studies. An analysis of the available trials show that PBC has a GI safety profile, which is better than that displayed by uncomplexed piroxicam. Being an inclusion complex of piroxicam, whose CV safety has been pointed out by several observational studies, PBC should be viewed as a CV safe anti-inflmmatory compound and a GI safer alternative to piroxicam. As a consequence, it should be considered as a useful addition to our therapeutic armamentarium. PMID:23394552

Urea loading enhances freezing survival and postfreeze recovery in a terrestrially hibernating frog.

PubMed

Costanzo, Jon P; Lee, Richard E

2008-09-01

We tested the hypothesis that urea, an osmolyte accumulated early in hibernation, functions as a cryoprotectant in the freeze-tolerant wood frog, Rana sylvatica. Relative to saline-treated, normouremic (10 micromol ml(-1)) frogs, individuals rendered hyperuremic (70 micromol ml(-1)) by administration of an aqueous urea solution exhibited significantly higher survival (100% versus 64%) following freezing at -4 degrees C, a potentially lethal temperature. Hyperuremic frogs also had lower plasma levels of intracellular proteins (lactate dehydrogenase, creatine kinase, hemoglobin), which presumably escaped from damaged cells, and more quickly recovered neurobehavioral functions following thawing. Experimental freezing-thawing did not alter tissue urea concentrations, but did elevate glucose levels in the blood and organs of all frogs. When measured 24 h after thawing commenced, glucose concentrations were markedly higher in urea-loaded frogs as compared to saline-treated ones, possibly because elevated urea retarded glucose clearance. Like other low-molecular-mass cryoprotectants, urea colligatively reduces both the amount of ice forming within the body and the osmotic dehydration of cells. In addition, by virtue of certain non-colligative properties, it may bestow additional protection from freeze-thaw damage not afforded by glucose.

Silver nanoparticles protect human keratinocytes against UVB radiation-induced DNA damage and apoptosis: potential for prevention of skin carcinogenesis

PubMed Central

Arora, Sumit; Tyagi, Nikhil; Bhardwaj, Arun; Rusu, Lilia; Palanki, Rohan; Vig, Komal; Singh, Shree R.; Singh, Ajay P.; Palanki, Srinivas; Miller, Michael E.; Carter, James E.; Singh, Seema

2015-01-01

Ultraviolet (UV)-B radiation from the sun is an established etiological cause of skin cancer, which afflicts more than a million lives each year in the United States alone. Here, we tested the chemopreventive efficacy of silver-nanoparticles (AgNPs) against UVB-irradiation-induced DNA damage and apoptosis in human immortalized keratinocytes (HaCaT). AgNPs were synthesized by reduction-chemistry and characterized for their physicochemical properties. AgNPs were well tolerated by HaCaT cells and their pretreatment protected them from UVB-irradiation-induced apoptosis along with significant reduction in cyclobutane-pyrimidine-dimer formation. Moreover, AgNPs pre-treatment led to G1-phase cell-cycle arrest in UVB-irradiated HaCaT cells. AgNPs were efficiently internalized in UVB-irradiated cells and localized into cytoplasmic and nuclear compartments. Furthermore, we observed an altered expression of various genes involved in cell-cycle, apoptosis and nucleotide-excision repair in HaCaT cells treated with AgNPs prior to UVB-irradiation. Together, these findings provide support for potential utility of AgNPs as novel chemopreventive agents against UVB-irradiation-induced skin carcinogenesis. PMID:25804413

Theoretical Estimation of Thermal Effects in Drilling of Woven Carbon Fiber Composite

PubMed Central

Díaz-Álvarez, José; Olmedo, Alvaro; Santiuste, Carlos; Miguélez, María Henar

2014-01-01

Carbon Fiber Reinforced Polymer (CFRPs) composites are extensively used in structural applications due to their attractive properties. Although the components are usually made near net shape, machining processes are needed to achieve dimensional tolerance and assembly requirements. Drilling is a common operation required for further mechanical joining of the components. CFRPs are vulnerable to processing induced damage; mainly delamination, fiber pull-out, and thermal degradation, drilling induced defects being one of the main causes of component rejection during manufacturing processes. Despite the importance of analyzing thermal phenomena involved in the machining of composites, only few authors have focused their attention on this problem, most of them using an experimental approach. The temperature at the workpiece could affect surface quality of the component and its measurement during processing is difficult. The estimation of the amount of heat generated during drilling is important; however, numerical modeling of drilling processes involves a high computational cost. This paper presents a combined approach to thermal analysis of composite drilling, using both an analytical estimation of heat generated during drilling and numerical modeling for heat propagation. Promising results for indirect detection of risk of thermal damage, through the measurement of thrust force and cutting torque, are obtained. PMID:28788685

Radiation-induced melting in coherent X-ray diffractive imaging at the nanoscale

PubMed Central

Ponomarenko, O.; Nikulin, A. Y.; Moser, H. O.; Yang, P.; Sakata, O.

2011-01-01

Coherent X-ray diffraction techniques play an increasingly significant role in the imaging of nanoscale structures, ranging from metallic and semiconductor to biological objects. In material science, X-rays are usually considered to be of a low-destructive nature, but under certain conditions they can cause significant radiation damage and heat loading on the samples. The qualitative literature data concerning the tolerance of nanostructured samples to synchrotron radiation in coherent diffraction imaging experiments are scarce. In this work the experimental evidence of a complete destruction of polymer and gold nanosamples by the synchrotron beam is reported in the case of imaging at 1–10 nm spatial resolution. Numerical simulations based on a heat-transfer model demonstrate the high sensitivity of temperature distribution in samples to macroscopic experimental parameters such as the conduction properties of materials, radiation heat transfer and convection. However, for realistic experimental conditions the calculated rates of temperature rise alone cannot explain the melting transitions observed in the nanosamples. Comparison of these results with the literature data allows a specific scenario of the sample destruction in each particular case to be presented, and a strategy for damage reduction to be proposed. PMID:21685675

Ceramic Composite Development for Gas Turbine Engine Hot Section Components

NASA Technical Reports Server (NTRS)

DiCarlo, James A.; VANrOODE, mARK

2006-01-01

The development of ceramic materials for incorporation into the hot section of gas turbine engines has been ongoing for about fifty years. Researchers have designed, developed, and tested ceramic gas turbine components in rigs and engines for automotive, aero-propulsion, industrial, and utility power applications. Today, primarily because of materials limitations and/or economic factors, major challenges still remain for the implementation of ceramic components in gas turbines. For example, because of low fracture toughness, monolithic ceramics continue to suffer from the risk of failure due to unknown extrinsic damage events during engine service. On the other hand, ceramic matrix composites (CMC) with their ability to display much higher damage tolerance appear to be the materials of choice for current and future engine components. The objective of this paper is to briefly review the design and property status of CMC materials for implementation within the combustor and turbine sections for gas turbine engine applications. It is shown that although CMC systems have advanced significantly in thermo-structural performance within recent years, certain challenges still exist in terms of producibility, design, and affordability for commercial CMC turbine components. Nevertheless, there exist some recent successful efforts for prototype CMC components within different engine types.

41 CFR 102-37.250 - What actions must a SASP take when it learns of damage to or loss of surplus property in its...

Code of Federal Regulations, 2010 CFR

2010-07-01

... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false What actions must a SASP take when it learns of damage to or loss of surplus property in its custody? 102-37.250 Section 102-37... learns of damage to or loss of surplus property in its custody? If you learn that surplus property in...

41 CFR 102-37.250 - What actions must a SASP take when it learns of damage to or loss of surplus property in its...

Code of Federal Regulations, 2011 CFR

2011-01-01

... 41 Public Contracts and Property Management 3 2011-01-01 2011-01-01 false What actions must a SASP take when it learns of damage to or loss of surplus property in its custody? 102-37.250 Section 102-37... learns of damage to or loss of surplus property in its custody? If you learn that surplus property in...

Engineering microbes for tolerance to next-generation biofuels

PubMed Central

2011-01-01

A major challenge when using microorganisms to produce bulk chemicals such as biofuels is that the production targets are often toxic to cells. Many biofuels are known to reduce cell viability through damage to the cell membrane and interference with essential physiological processes. Therefore, cells must trade off biofuel production and survival, reducing potential yields. Recently, there have been several efforts towards engineering strains for biofuel tolerance. Promising methods include engineering biofuel export systems, heat shock proteins, membrane modifications, more general stress responses, and approaches that integrate multiple tolerance strategies. In addition, in situ recovery methods and media supplements can help to ease the burden of end-product toxicity and may be used in combination with genetic approaches. Recent advances in systems and synthetic biology provide a framework for tolerance engineering. This review highlights recent targeted approaches towards improving microbial tolerance to next-generation biofuels with a particular emphasis on strategies that will improve production. PMID:21936941

Arguments for zero tolerance of sexual contact between doctors and patients.

PubMed Central

Cullen, R M

1999-01-01

Some doctors do enter into sexual relationships with patients. These relationships can be damaging to the patient involved. One response available to both individual doctors and to disciplinary bodies is to prohibit sexual contact between doctors and patients ("zero tolerance"). This paper considers five ways of arguing for a zero tolerance policy. The first rests on an empirical claim that such contact is almost always harmful to the patient involved. The second is based on a "principles" approach while the third originates in "virtues" ethics. The fourth argues that zero tolerance is an "a priori" truth. These four attempt to establish that the behaviour is always wrong and ought, therefore, to be prohibited. The fifth argument is counterfactual. It claims a policy that allowed sexual contact would have unacceptable consequences. Given the responsibility of regulatory bodies to protect the public, zero tolerance is a natural policy to develop. PMID:10635503

RecA-mediated SOS response provides a geraniol tolerance in Escherichia coli.

PubMed

Shah, Asad Ali; Wang, Chonglong; Yoon, Sang-Hwal; Kim, Jae-Yean; Choi, Eui-Sung; Kim, Seon-Won

2013-09-20

Geraniol is an important industrial material and a potential candidate of advanced biofuels. One challenge of microbial geraniol production is the toxicity to hosts. However, the poor understanding on geraniol tolerance mechanism is an obstacle for developing geraniol tolerant host. This study genome-widely screened a shot-gun DNA library of Escherichia coli and found that recA is able to confer geraniol tolerance in E. coli. The recA knockout mutant was found extremely sensitive to geraniol. Based on our data, it was deciphered that recA provided tolerance through SOS response network responding to DNA damage caused by geraniol. RecA-mediated SOS response activates the homologous recombinational repair by RecB and RecN for corrective DNA maintenance. This protection mechanism suggests an effective strategy to combat geraniol toxicity in E. coli. Copyright © 2013 Elsevier B.V. All rights reserved.

Does lipophilicity of toxic compounds determine effects on drought tolerance of the soil collembolan Folsomia candida?

PubMed

Skovlund, Gitte; Damgaard, Christian; Bayley, Mark; Holmstrup, Martin

2006-12-01

The ability of Collembola to survive drought stress is crucial for their distribution in the terrestrial environment. Previous studies have suggested that several toxic compounds affect the drought tolerance of Folsomia candida in a synergistic manner and that these compounds have the feature in common that they elicit their toxicity by causing membrane damage. We hypothesised that the detrimental effect of toxic chemicals on drought tolerance in F. candida depends on the lipophilicity (log K(ow)) of the compound because a higher log K(ow) would mean a closer interaction with membranes. In this study the three chemicals 4-nonylphenol, pyrene and p,p'-DDE were tested. Surprisingly, 4-nonylphenol, with the lowest log K(ow), was the most potent with respect to reducing drought tolerance followed by pyrene, suggesting that interactions between drought tolerance and chemical stress do not depend on lipophilicity alone.

Cell Injury and Repair Resulting from Sleep Loss and Sleep Recovery in Laboratory Rats

PubMed Central

Everson, Carol A.; Henchen, Christopher J.; Szabo, Aniko; Hogg, Neil

2014-01-01

Study Objectives: Increased cell injury would provide the type of change in constitution that would underlie sleep disruption as a risk factor for multiple diseases. The current study was undertaken to investigate cell injury and altered cell fate as consequences of sleep deprivation, which were predicted from systemic clues. Design: Partial (35% sleep reduction) and total sleep deprivation were produced in rats for 10 days, which was tolerated and without overtly deteriorated health. Recovery rats were similarly sleep deprived for 10 days, then allowed undisturbed sleep for 2 days. The plasma, liver, lung, intestine, heart, and spleen were analyzed and compared to control values for damage to DNA, proteins, and lipids; apoptotic cell signaling and death; cell proliferation; and concentrations of glutathione peroxidase and catalase. Measurements and Results: Oxidative DNA damage in totally sleep deprived rats was 139% of control values, with organ-specific effects in the liver (247%), lung (166%), and small intestine (145%). Overall and organ-specific DNA damage was also increased in partially sleep deprived rats. In the intestinal epithelium, total sleep deprivation resulted in 5.3-fold increases in dying cells and 1.5-fold increases in proliferating cells, compared with control. Two days of recovery sleep restored the balance between DNA damage and repair, and resulted in normal or below-normal metabolic burdens and oxidative damage. Conclusions: These findings provide physical evidence that sleep loss causes cell damage, and in a manner expected to predispose to replication errors and metabolic abnormalities; thereby providing linkage between sleep loss and disease risk observed in epidemiological findings. Properties of recovery sleep include biochemical and molecular events that restore balance and decrease cell injury. Citation: Everson CA, Henchen CJ, Szabo A, Hogg N. Cell injury and repair resulting from sleep loss and sleep recovery in laboratory rats. SLEEP 2014;37(12):1929-1940. PMID:25325492

Optimal Design and Damage Tolerance Verification of an Isogrid Structure for Helicopter Application

NASA Technical Reports Server (NTRS)

Baker, Donald J.; Fudge, Jack; Ambur, Damodar R.; Kassapoglou, Christos

2003-01-01

A composite isogrid panel design for application to a rotorcraft fuselage is presented. An optimum panel design for the lower fuselage of the rotorcraft that is subjected to combined in-plane compression and shear loads was generated using a design tool that utilizes a smeared-stiffener theory in conjunction with a genetic algorithm. A design feature was introduced along the edges of the panel that facilitates introduction of loads into the isogrid panel without producing undesirable local bending gradients. A low-cost manufacturing method for the isogrid panel that incorporates these design details is also presented. Axial compression tests were conducted on the undamaged and low-speed impact damaged panels to demonstrate the damage tolerance of this isogrid panel. A combined loading test fixture was designed and utilized that allowed simultaneous application of compression and shear loads to the test specimen. Results from finite element analyses are presented for the isogrid panel designs and these results are compared with experimental results. This study illustrates the isogrid concept to be a viable candidate for application to the helicopter lower fuselage structure.

Increase of cold tolerance in cotton plant (Gossypium hirsutum L.) by mepiquat chloride

NASA Technical Reports Server (NTRS)

Gausman, H. W.; Escobar, D. E.; Rodriguez, R. R. (Principal Investigator); Huang, S. Y.; Rittig, F. R.

1982-01-01

Three mepiquat chloride (MC) concentrations - 40, 70, and 100 g a.i./ha - were used to spray cotton (Gossypium hirsutum L., cultival McNair 220) plants to determine whether or not MC would increase their cold tolerance. Seven to ten days after the spray, the plants were exposed to three different cold treatments. No important difference in cold damage was noticed between the control and the MC-treated plants when they were exposed repeatedly to 4.5 C. No plants died when exposed to 0.5 C for 12 h; however, 90% of the 1st and 2nd leaves of the control plants were damaged. This was three times more damage than those leaves of plants treated with 70 and 100 g a.i./ha MC concentrations; 60% f the control and 10-20% of the MC-treated plants died when the plants were subjected to a cold hardening process with 15.5 C day (12 h) and 1.7 C night (12 h) for 10 days, and then, held at -2.2 C for 24 hours. The electrolyte leakage and reflectance measurement data showed that the cell membranes of the MC-treated plants sustained much less damage than those of the control. Freezing injury was easily assessed by reflectance measurements at the 1.65 micrometer wavelength.

Homologous Recombination and Translesion DNA Synthesis Play Critical Roles on Tolerating DNA Damage Caused by Trace Levels of Hexavalent Chromium

PubMed Central

Chen, Youjun; Zhou, Yi-Hui; Neo, Dayna; Clement, Jean; Takata, Minoru; Takeda, Shunichi; Sale, Julian; Wright, Fred A.; Swenberg, James A.; Nakamura, Jun

2016-01-01

Contamination of potentially carcinogenic hexavalent chromium (Cr(VI)) in the drinking water is a major public health concern worldwide. However, little information is available regarding the biological effects of a nanomoler amount of Cr(VI). Here, we investigated the genotoxic effects of Cr(VI) at nanomoler levels and their repair pathways. We found that DNA damage response analyzed based on differential toxicity of isogenic cells deficient in various DNA repair proteins is observed after a three-day incubation with K2CrO4 in REV1-deficient DT40 cells at 19.2 μg/L or higher as well as in TK6 cells deficient in polymerase delta subunit 3 (POLD3) at 9.8 μg/L or higher. The genotoxicity of Cr(VI) decreased ~3000 times when the incubation time was reduced from three days to ten minutes. TK mutation rate also significantly decreased from 6 day to 1 day exposure to Cr(VI). The DNA damage response analysis suggest that DNA repair pathways, including the homologous recombination and REV1- and POLD3-mediated error-prone translesion synthesis pathways, are critical for the cells to tolerate to DNA damage caused by trace amount of Cr(VI). PMID:27907204

Role of jasmonic acid in improving tolerance of rapeseed (Brassica napus L.) to Cd toxicity*

PubMed Central

Ali, Essa; Hussain, Nazim; Shamsi, Imran Haider; Jabeen, Zahra; Siddiqui, Muzammil Hussain; Jiang, Li-xi

2018-01-01

The well-known detrimental effects of cadmium (Cd) on plants are chloroplast destruction, photosynthetic pigment inhibition, imbalance of essential plant nutrients, and membrane damage. Jasmonic acid (JA) is an alleviator against different stresses such as salinity and drought. However, the functional attributes of JA in plants such as the interactive effects of JA application and Cd on rapeseed in response to heavy metal stress remain unclear. JA at 50 μmol/L was observed in literature to have senescence effects in plants. In the present study, 25 μmol/L JA is observed to be a “stress ameliorating molecule” by improving the tolerance of rapeseed plants to Cd toxicity. JA reduces the Cd uptake in the leaves, thereby reducing membrane damage and malondialdehyde content and increasing the essential nutrient uptake. Furthermore, JA shields the chloroplast against the damaging effects of Cd, thereby increasing gas exchange and photosynthetic pigments. Moreover, JA modulates the antioxidant enzyme activity to strengthen the internal defense system. Our results demonstrate the function of JA in alleviating Cd toxicity and its underlying mechanism. Moreover, JA attenuates the damage of Cd to plants. This study enriches our knowledge regarding the use of and protection provided by JA in Cd stress. PMID:29405041