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Sample records for danish pig slaughter

  1. Trends in slaughter pig production and antimicrobial consumption in Danish slaughter pig herds, 2002-2008.

    PubMed

    Vieira, A R; Pires, S M; Houe, H; Emborg, H-D

    2011-10-01

    Overuse of antimicrobials in food-animal production is thought to be a major risk factor for the development of resistant bacterial populations. Data on non-human antimicrobial usage is essential for planning of intervention strategies to lower resistance levels at the country, region or herd levels. In this study we evaluated Danish national antimicrobial usage data for five antimicrobial classes used in slaughter pigs in different herd sizes and data on the number of slaughter pigs produced per herd, between 2002 and 2008, in Denmark. The objective was to ascertain if there is an association between herd size and amount of antimicrobials consumed. During this period, the overall number of herds with slaughter pigs decreased by 43%, with larger herds becoming more prevalent. The tetracycline treatment incidence (TI) rate increased from 0·28 to 0·70 animal-defined daily dose (ADD)/100 slaughter pig-days at risk while macrolide TI presented a more moderate increase, from 0·40 to 0·44 ADD/100 slaughter pig-days at risk during the study period. Linear regression analyses revealed a significant association between herd size and TI rates for tetracyclines, macrolides, sulfonamides/trimethoprim and cephalosporins, with small herds presenting significantly higher TI than moderate, large and the largest herds. This study highlights the importance of establishing an antimicrobial consumption monitoring programme, integrated with comprehensive food-animal production surveillance. Further research should be performed to address the potential causes of the detected associations between herd sizes and antimicrobial consumption in pigs.

  2. Manufacturing technology in the Danish pig slaughter industry.

    PubMed

    Hinrichsen, Lars

    2010-02-01

    The Danish pig meat industry is very export oriented. Ninety per cent of the production of the big cooperative slaughterhouses is exported to more than 100 countries all over the world. This poses a requirement for the industry to be globally competitive in the sense of quality, product safety and--of course--price. A big challenge for the industry is therefore to maintain sufficient low unit costs in spite of the high factor costs of Denmark. In particular the high labour costs must be accompanied by correspondingly high labour productivity. And, it should be emphasized, this high labour productivity must be achieved without compromising the concern for good working conditions of the employees in the manufacturing. Technology is one of the means to achieve this combination of good working conditions and high labour productivity. One of the most important benefits from automation is the improved working environment. Pig slaughtering, cutting and boning is traditionally very labour intensive and requires hard and repetitive work. For many people a job in a slaughterhouse is therefore not their first choice. This situation can be changed by automation, which will not only reduce arduous and repetitive work but in addition will introduce more motivating jobs in terms of planning, supervision and control of the new technology. Automation will also improve the hygiene and thereby the food safety. This applies in particular to the clean slaughter line where cross contamination between carcasses is reduced because of less manual handling and because the tools in the machines can be sterilised more effectively between each carcass. Automated processes are more accurate and repeatable than manual work. For some processes, in particular in cutting and boning, this will enhance the product yield. New technology can also improve the animal welfare. The group-stunning system and mechanised lairage systems are examples of that. Improved animal welfare has an ethical value in

  3. Evaluation of the antibacterial residue surveillance programme in Danish pigs using Bayesian methods.

    PubMed

    Baptista, F M; Alban, L; Olsen, A M; Petersen, J V; Toft, N

    2012-10-01

    Residues of pharmacological active substances or their metabolites might be found in food products from food-producing animals. Maximum Residue Limits for pharmacological active substances in foodstuffs of animal origin are established to assure high food safety standards. Each year, more than 20,000 samples are analysed for the presence of antibacterial residues in Danish pigs. This corresponds to 0.1% of the size of the slaughter pig population and more than 1% of the sows slaughtered. In this study, a Bayesian model was used to evaluate the Danish surveillance system accuracy and to investigate the impact of a potential risk-based sampling approach to the residue surveillance programme in Danish slaughter pigs. Danish surveillance data from 2005 to 2009 and limited knowledge about true prevalence and test sensitivity and specificity were included in the model. According to the model, the true antibacterial residue prevalence in Danish pigs is very low in both sows (∼0.20%) and slaughter pigs (∼0.01%). Despite data constraints, the results suggest that the current screening test used in Denmark presents high sensitivity (85-99%) and very high specificity (>99%) for the most relevant antibacterial classes used in Danish pigs. If high-risk slaughter pigs could be identified by taking into account antibacterial use or meat inspection risk factors, a potential risk-based sampling approach to antibacterial residue surveillance in slaughter pigs would allow reducing the sample size substantially, while increasing or maintaining the probability of detection. Hence, the antibacterial residue surveillance programme in Danish pigs would be more cost-effective than today.

  4. Seroprevalence of enteropathogenic Yersinia spp. in pig batches at slaughter.

    PubMed

    Vanantwerpen, Gerty; Van Damme, Inge; De Zutter, Lieven; Houf, Kurt

    2014-09-01

    Enteropathogenic Yersinia spp. are one of the main causes of foodborne bacterial infections in Europe. Slaughter pigs are the main reservoir and carcasses are contaminated during a sub-optimal hygienically slaughtering-process. Serology is potentially an easy option to test for the Yersinia-status of the pig (batches) before slaughter. A study of the variation in activity values (OD%) of Yersinia spp. in pigs and pig batches when applying a serological test were therefore conducted. In this study, pieces of the diaphragm of 7047 pigs, originating from 100 farms, were collected and meat juice was gathered, where after an enzyme-linked immunosorbent assay (ELISA) Pigtype Yopscreen (Labor Diagnostik Leipzig, Qiagen, Leipzig, Germany) was performed. The results were defined positive if the activity values exceeded the proposed cut-off value of 30 OD%. Results at pig level displayed a bimodal-shaped distribution with modes at 0-10% (n=879) and 50-60% (n=667). The average OD% was 51% and 66% of the animals tested positive. The within-batch seroprevalence ranged from 0 to 100% and also showed a bimodal distribution with modes at 0% (n=7) and 85-90% (n=16). On 7 farms, no single seropositive animal was present and in 22 farms, the mean OD% was below 30%. Based on the results obtained at slaughter, 66% of the pigs had contact with enteropathogenic Yersinia spp. at farm level. The latter occurred in at least 93% of the farms indicating that most farms are harboring enteropathogenic Yersinia spp.

  5. Mycobacterium fortuitum from lesions of slaughtered pigs in Ibadan, Nigeria.

    PubMed

    Cadmus, S I B; Adesokan, H K; Okker, M; Jahans, K

    2010-12-01

    To ascertain the cause of tuberculous-like lesions in pigs slaughtered in a local abattoir in Ibadan (south-western Nigeria), a total of 516 pigs were inspected over a period of four months, 18 of which had gross lesions suggestive of tuberculosis at post-mortem. Mycobacterial culture and molecular typing (GenoType Mycobacterium CM [Common Mycobacteria] assay) analysis were used to identify and confirm the mycobacteria species responsible for these lesions. Results show that 2.3% (12/516) of the animals screened were infected with mycobacteria; Mycobacterium fortuitum was confirmed in 33.3% (4/12) of these cases. As far as the authors are aware, this is the first report confirming the isolation of M. fortuitum in slaughtered pigs in Nigeria. There is a need to improve on necessary preventive and control measures that will reduce potential sources of mycobacterial infections in pig-rearing herds. These infections may also have public health implications, especially to workers in the pig industry.

  6. Welfare of entire male pigs is improved by socialising piglets and keeping intact groups until slaughter.

    PubMed

    Rydhmer, L; Hansson, M; Lundström, K; Brunius, C; Andersson, K

    2013-09-01

    In today's production systems, pigs raised for slaughter are mixed many times, resulting in stress and fighting. The negative consequences of mixing are probably more severe with entire males than with castrates, as they fight more. In this project, we studied a system without castration where entire male pigs met unfamiliar pigs only once. Piglets from two litters were allowed to visit each other from circa 2 weeks of age through an opening between the farrowing pens. Entire males from these litters were kept in intact groups from weaning and onwards, and they were slaughtered pen-wise in intact groups. Control pigs were raised and weaned in their litters and mixed with unknown pigs when moved to the growing-finishing unit. They were slaughtered by split marketing based on individual weight. In total, 96 entire males from 24 litters were studied. Activity and social interactions of pigs were studied by direct observations on three observation occasions per pen for pigs kept in intact groups and four occasions for control pigs. All pigs were inspected for skin lesions during raising and at slaughter. Results showed that fewer pigs in intact groups were resting (17.1% v. 28.5%; P = 0.044) and they showed less aggressive behaviour (16.1 v. 27.7 number of interactions per hour; P = 0.001) than control pigs when moved to the growing-finishing unit. They also got fewer skin lesions compared with control pigs (15 v. 35; P < 0.001). Consequently, control pigs tended to grow slower during the 1st week after mixing; however, growth rate during the whole growing-finishing phase did not differ between treatments (P = 0.205). Control pigs directed more aggressive behaviour towards non-litter mates than towards litter mates during the whole growing-finishing phase, whereas pigs from the other treatment made no difference between litter mates and other familiar pigs. At 67 kg, there was more sexual behaviour (mounting) among control pigs (7.6 v. 3.4; P = 0.033), but after

  7. Sero- and genotyping of Salmonella in slaughter pigs, from farm to cutting plant, with a focus on the slaughter process.

    PubMed

    Arguello, Héctor; Carvajal, Ana; Naharro, German; Arcos, Mario; Rodicio, M Rosario; Martin, M Cruz; Rubio, Pedro

    2013-01-15

    The objective of this study was to investigate the role of the slaughtering process in Salmonella carcass contamination by typing isolates recovered previously in a double study of the following: (1) a tracking survey from the farm to the slaughterhouse and (2) a survey of the slaughterhouse environment (i.e., lairage area, slaughter line, cutting plant and carcasses). The Salmonella serotypes identified on the carcasses of the 16 tracked batches were frequently linked to lairage, whereas the serotypes detected at the farm, transport or pig-related samples (i.e., caecum content and lymph nodes) were only occasionally detected at the carcass level. Multi-locus variable-number tandem repeats (MLVA) of 77 Salmonella enterica ser. Typhimurium isolates from seven of these batches confirmed the link between the isolates recovered from carcasses and holding pens. Only four of the 16 positive carcasses had profiles previously isolated from lymph nodes or caecal content. In the second part of the study, a total of 131 S. enterica ser. Typhimurium and 74 S. enterica ser. Derby isolates were further characterised by MLVA and Pulsed Field Gel Electrophoresis (PFGE), respectively. The MLVA profiles identified in carcasses varied throughout the working day and were frequently linked to those identified in samples from the slaughter line points collected close in time. PFGE and MLVA profiles identified at lairage were also detected in later processing facilities (i.e., slaughter line and cutting plant) as well as in carcasses. Finally, most of the profiles found at the cutting plants were previously identified in the slaughter line or carcass samples. The results from this study show that Salmonella contamination in pigs entering the slaughterhouse can be attributed to several sources. Typing of isolates by MLVA and PFGE clarified the sources of carcass contamination and improved the accuracy of cross-contamination attributable values. Without obviating the relevant role of

  8. Pigs' aggressive temperament affects pre-slaughter mixing aggression, stress and meat quality.

    PubMed

    D'Eath, R B; Turner, S P; Kurt, E; Evans, G; Thölking, L; Looft, H; Wimmers, K; Murani, E; Klont, R; Foury, A; Ison, S H; Lawrence, A B; Mormède, P

    2010-04-01

    Pre-slaughter stress has a negative impact on animal welfare and on meat quality. Aggressive behaviour when pigs are mixed together for transportation to, or on arrival at, the abattoir is an important factor in pre-slaughter stress. Aggressiveness of pigs varies between individuals in the population, and this study investigated its effects on stress and meat quality at slaughter. We mixed pigs at a young age to identify individuals of high (H) or low (L) aggressive temperament using the previously validated approach of lesion scoring. To contrast extremes of social stress single-sex groups of eight pigs were mixed according to their aggressiveness in HH, HL or LL combinations or left unmixed (U) prior to transport and slaughter (n = 271). Each treatment was replicated in at least two groups in each of four slaughter batches. Mixing per se had little effect, but mixed groups composed of aggressive pigs (HH) had more carcass skin lesions and higher levels of plasma cortisol at slaughter and had loin muscle samples with higher pH at 24 h, and lower redness (a*) and yellowness (b*) compared to the other treatments. Females had higher levels of plasma cortisol at slaughter, a more rapid decline in pH post-slaughter and greater lean content of meat. Lactate and creatine kinase (CK) levels and meat pH were affected by the interaction of sex and treatment. Genetic factors, dam and sire line composition, and halothane locus (ryanodine receptor 1, RYR1) genotype, also affected a number of production and meat quality parameters as expected. Additionally, 'commercially normal' levels of social stress were studied in four further slaughter batches with no manipulation of group composition (n = 313). In these pigs, the proportion of unfamiliar pigs and group size of lairage groups explained limited variation in lesion scores at slaughter, but earlier aggressiveness did not. High numbers of skin lesions on the carcass were associated with high levels of cortisol and lactate and

  9. Within-herd biosecurity and Salmonella seroprevalence in slaughter pigs: a simulation study.

    PubMed

    Lurette, A; Touzeau, S; Ezanno, P; Hoch, T; Seegers, H; Fourichon, C; Belloc, C

    2011-07-01

    In Europe, on-farm biosecurity measures, involving a strict all-in/all-out batch-management system and decontamination of the rearing rooms between consecutive batches, are recommended to control Salmonella infection in growing pigs. However, implementation of these measures is often relaxed under common farming conditions. Therefore, this study was conducted to assess the relative contributions of batch-management system and room decontamination efficacy on Salmonella seroprevalence for different growing rates and subsequent slaughter ages of pigs. Because the impact of these factors cannot be easily evaluated by an observational approach in commercial farms, a stochastic simulation model representing the population dynamics, herd management, and Salmonella infection within a farrow-to-finish pig herd was used. Realistic levels were set for each factor under study (3 for batch-management system and slaughter age; 4 for room decontamination) to generate 54 simulation scenarios. Salmonella shedding prevalence in groups of slaughter pigs was then compared. A sensitivity analysis was performed to rank the impacts of the 3 factors on output. Batch-management system had little effect. In contrast, room decontamination efficacy had the greatest impact on Salmonella prevalence in pigs at slaughter. A drop in decontamination efficacy from 100 to 50%, with a strict all-in/all-out batch-management system and for all slaughter ages tested, noticeably increased (P<0.001) the prevalence and almost doubled it for the reference slaughter age. Our results suggest that the control of Salmonella in pig herds should primarily focus on room decontamination efficacy. Provided that a good level of room decontamination is ensured, some flexibility in batch management, in terms of pig mixing, would be acceptable to limit the number of underweight pigs delivered to the slaughterhouse.

  10. NMR-based metabonomics reveals relationship between pre-slaughter exercise stress, the plasma metabolite profile at time of slaughter, and water-holding capacity in pigs.

    PubMed

    Bertram, H C; Oksbjerg, N; Young, J F

    2010-01-01

    Nuclear magnetic resonance (NMR)-based metabonomics was applied to investigate the effects of pre-slaughter exercise stress on the plasma metabolite profile at time of slaughter. The study included a total of 40 slaughter pigs, which were exposed to one of the following treatments: No pre-slaughter stress (control treatment), pre-slaughter exercise on a treadmill and subsequently 0, 1, or 3h rest prior to slaughter. NMR-based metabonomics revealed a clear difference in the plasma metabolite profile at time of slaughter between control pigs and pigs exercised without rest, which mainly could be ascribed to increased plasma lactate due to exercise. A resting period of 1 or 3h prior to slaughter reversed the stress-induced perturbations in the plasma metabolite profile. The plasma metabolite profile at time of slaughter was highly correlated with muscle temperature 1 min post-mortem, and a correlation to WHC was also demonstrated. Lactate was found to be the metabolite of importance for the association between the plasma metabolome and pH, temperature and WHC.

  11. Influence of breed and slaughter weight on boar taint prevalence in entire male pigs.

    PubMed

    Aluwé, M; Millet, S; Bekaert, K M; Tuyttens, F A M; Vanhaecke, L; De Smet, S; De Brabander, D L

    2011-06-01

    Piétrain (P), Large White (LW) and Belgian Landrace stress negative (BN) boars were slaughtered at 50, 70, 90 or 110 kg live weight to investigate breed differences and the effect of slaughter weight on boar taint prevalence. Boar taint was quantified by four different methodologies: sensory evaluation of neckfat heated with a hot iron in the slaughterhouse, sensory evaluation of meat by consumer panels, sensory evaluation of fat and meat by expert panels and laboratory analysis of indole, skatole and androstenone in backfat. Skatole levels in backfat were significantly higher for LW and BN than for P boars. The androstenone levels and the hot iron method revealed a significant interaction between breed and slaughter weight. On the other hand, experts detected an effect of weight on the androstenone odour perception, which was significantly higher in fat from boars slaughtered at 90 kg compared with 50 kg, and significantly higher in meat from boars slaughtered at 110 kg compared with 50 kg. Consumers did not detect differences in the sensory characteristics among breeds or slaughter weight. These results indicate opportunities to minimise the risk of boar taint in entire male pigs by carefully selecting a combination of breed and slaughter weight. Along with the optimal slaughter weight, the effectiveness of reducing boar taint by lowering slaughter weight appeared to be breed dependent.

  12. Investigation Into the Humaneness of Slaughter Methods for Guinea Pigs (Cavia porcelus) in the Andean Region

    PubMed Central

    Limon, Georgina; Gonzales-Gustavson, Eloy A.; Gibson, Troy J.

    2016-01-01

    Guinea pigs (Cavia porcelus) are an important source of nonhuman animal protein in the Andean region of South America. Specific guidelines regarding the welfare of guinea pigs before and during slaughter have yet to be developed. This study critically assessed the humaneness of 4 different stunning/slaughter methods for guinea pigs: cervical neck dislocation (n = 60), electrical head-only stunning (n = 83), carbon dioxide (CO2) stunning (n = 21), and penetrating captive bolt (n = 10). Following cervical neck dislocation, 97% of guinea pigs had at least 1 behavioral or cranial/spinal response. Six percent of guinea pigs were classified as mis-stunned after electrical stunning, and 1% were classified as mis-stunned after captive bolt. Increased respiratory effort was observed during CO2 stunning. Apart from this finding, there were no other obvious behavioral responses that could be associated with suffering. Of the methods assessed, captive bolt was deemed the most humane, effective, and practical method of stunning guinea pigs. Cervical neck dislocation should not be recommended as a slaughter method for guinea pigs. PMID:26963642

  13. Investigation Into the Humaneness of Slaughter Methods for Guinea Pigs (Cavia porcelus) in the Andean Region.

    PubMed

    Limon, Georgina; Gonzales-Gustavson, Eloy A; Gibson, Troy J

    2016-01-01

    Guinea pigs (Cavia porcelus) are an important source of nonhuman animal protein in the Andean region of South America. Specific guidelines regarding the welfare of guinea pigs before and during slaughter have yet to be developed. This study critically assessed the humaneness of 4 different stunning/slaughter methods for guinea pigs: cervical neck dislocation (n = 60), electrical head-only stunning (n = 83), carbon dioxide (CO2) stunning (n = 21), and penetrating captive bolt (n = 10). Following cervical neck dislocation, 97% of guinea pigs had at least 1 behavioral or cranial/spinal response. Six percent of guinea pigs were classified as mis-stunned after electrical stunning, and 1% were classified as mis-stunned after captive bolt. Increased respiratory effort was observed during CO2 stunning. Apart from this finding, there were no other obvious behavioral responses that could be associated with suffering. Of the methods assessed, captive bolt was deemed the most humane, effective, and practical method of stunning guinea pigs. Cervical neck dislocation should not be recommended as a slaughter method for guinea pigs.

  14. Analysis of factors associated with mortality of heavy slaughter pigs during transport and lairage.

    PubMed

    Vitali, A; Lana, E; Amadori, M; Bernabucci, U; Nardone, A; Lacetera, N

    2014-11-01

    The study was based on data collected during 5 yr (2003-2007) and was aimed at assessing the effects of the month, slaughter house of destination (differing for stocking density, openings, brightness, and cooling device types), length of the journey, and temperature-humidity index (THI) on mortality of heavy slaughter pigs (approximately 160 kg live weight) during transport and lairage. Data were obtained from 24,098 journeys and 3,676,153 pigs transported from 1,618 farms to 3 slaughter houses. Individual shipments were the unit of observation. The terms dead on arrival (DOA) and dead in pen (DIP) refer to pigs that died during transport and in lairage at the abattoir before slaughtering, respectively. These 2 variables were assessed as the dependent counts in separate univariate Poisson regressions. The independent variables assessed univariately in each set of regressions were month of shipment, slaughter house of destination, time traveled, and each combination of the month with the time traveled. Two separate piecewise regressions were done. One used DOA counts within THI levels over pigs transported as a dependent ratio and the second used DIP counts within THI levels over pigs from a transport kept in lairage as a dependent ratio. The THI was the sole independent variable in each case. The month with the greatest frequency of deaths was July with a risk ratio of 1.22 (confidence interval: 1.06-1.36; P < 0.05) and 1.27 (confidence interval: 1.06-1.51; P < 0.05) for DOA and DIP, respectively. The lower mortality risk ratios for DOA and DIP were recorded for January and March (P < 0.05). The aggregated data of the summer (June, July, and August) versus non-summer (January, March, September, and November) months showed a greater risk of pigs dying during the hot season when considering both transport and lairage (P < 0.05). The mortality risk ratio of DIP was lower at the slaughter house with the lowest stocking density (0.64 m(2)/100 kg live weight), large open

  15. Influence of On-farm pig Salmonella status on Salmonella Shedding at Slaughter.

    PubMed

    Casanova-Higes, A; Andrés-Barranco, S; Mainar-Jaime, R C

    2016-08-23

    The risk of Salmonella shedding among pigs at slaughter with regard to their previous on-farm Salmonella status was assessed in a group of pigs from a farm from NE of Spain. A total of 202 pigs that had been serologically monitored monthly during the fattening period and from which mesenteric lymph nodes (MLN) and faecal (SFEC) samples were collected at slaughter for Salmonella isolation were included. A repeated-measures anova was used to assess the relationship between mean OD% values during the fattening period and sampling time and bacteriology on MLN and SFEC. Pigs were also grouped into four groups, that is pigs seronegative during the fattening period and Salmonella negative in MLN (group A; n = 69); pigs seronegative during the fattening period but Salmonella positive in MLN (B; n = 36); pigs seropositive at least once and Salmonella positive in MLN (C; n = 50); and pigs seropositive at least once but Salmonella negative in (D; n = 47). Pigs shedding at slaughter seroconverted much earlier and showed much higher mean OD% values than non-shedders pigs. The proportion of Salmonella shedders in groups A and D was high and similar (26.1% and 29.8%, respectively), but significantly lower than that for groups B and C. The odds of shedding Salmonella for groups B and C were 4.8 (95% CI = 1.5-15.5) and 20.9 (3.7-118) times higher, respectively, when compared to A. It was concluded that a large proportion of Salmonella seronegative pigs may shed Salmonella at slaughter, which would be likely associated to previous exposure with contaminated environments (i.e. transport and lairage). For pigs already infected at farm, the likelihood of shedding Salmonella was much higher and may depend on whether the bacterium has colonized the MLN or not. The odds of shedding Salmonella spp. were always much higher for pigs in which Salmonella was isolated from MLN.

  16. Alternative rearing systems in pigs: consequences on stress indicators at slaughter and meat quality.

    PubMed

    Foury, A; Lebret, B; Chevillon, P; Vautier, A; Terlouw, C; Mormède, P

    2011-08-01

    The objective of this study was to evaluate the effects of three alternative (ALT) rearing systems for growing pigs (outdoor: 150 m2/pig; straw bedding: 1.30 m2/pig; and hut with access to a courtyard: 1.30 m2/pig) compared with a conventional system (fully slatted floor: 0.65 m2/pig, considered as control), on pre-slaughter stress indicators in relation with meat quality. To that end, the number of skin lesions on whole carcasses, as well as blood creatine kinase (CK) activity and urine levels in cortisol and catecholamines (adrenaline and noradrenaline) were determined at slaughter. Glycolytic potential (GP) and ultimate pH of the semimembranosus muscle were also measured. The global correlation network calculated between all these parameters shows that the indicators of pre-slaughter muscle activity (plasma CK) and/or stress indicators (e.g. adrenaline) are negatively (r=-0.26, P<0.01; r=-0.29, P<0.05, respectively) correlated with muscle GP and positively (r=0.17, P<0.05; r=0.44, P<0.001, respectively) with meat ultimate pH. Although some traits measured were sensitive to the degree of pre-slaughter mixing, they differed across rearing systems. The differences were most pronounced for the comparison of outdoors v. slatted floor. The lower levels of plasma CK and urinary catecholamines, and the lower number of carcass skin lesions of pigs reared outdoors, were related to a lower meat ultimate pH. Thus, ALT rearing systems influence animal welfare and meat quality, by providing enriched environmental conditions to the animals.

  17. Vaccination of pigs reduces Salmonella Typhimurium numbers in a model mimicking pre-slaughter stress.

    PubMed

    Leyman, Bregje; Boyen, Filip; Verbrugghe, Elin; Parys, Alexander Van; Haesebrouck, Freddy; Pasmans, Frank

    2012-11-01

    In pigs, infection with Salmonella Typhimurium often results in the development of carriers that re-excrete the organism during periods of stress. Previous studies have shown that cortisol plays a significant role in the recrudescence of Salmonella Typhimurium and that re-excretion can be induced by injections of dexamethasone. This study evaluated whether a commercially available Salmonella Typhimurium vaccine was able to reduce Salmonella excretion in a model mimicking pre-slaughter stress. Pigs were randomly assigned to either vaccination or a control group and, 5 weeks later, were infected with Salmonella Typhimurium. Twenty-three days post infection, pigs were injected with dexamethasone to induce recrudescence and Salmonella Typhimurium numbers were determined. Salmonella loads were significantly lower in the ileum and colon and in the contents of the ileum and caecum in vaccinated pigs than in non-vaccinated pigs. In addition, significantly more Salmonella positive tonsil and colon samples were found in non-vaccinated pigs. Vaccination with an attenuated vaccine reduced but did not eliminate Salmonella Typhimurium in pigs in conditions mimicking pre-slaughter stress.

  18. Prevalence and genetic diversity of enteropathogenic Yersinia spp. in pigs at farms and slaughter in Lithuania.

    PubMed

    Novoslavskij, Aleksandr; Šernienė, Loreta; Malakauskas, Alvydas; Laukkanen-Ninios, Riikka; Korkeala, Hannu; Malakauskas, Mindaugas

    2013-04-01

    The prevalence of enteropathogenic Yersinia spp. in pigs at farms and slaughter in relation to potential farming risk factors in Lithuania was examined. Pig faeces and carcase swab samples from 11 farms were studied at slaughterhouses. Nine of the 11 farms were visited again 3-5 months later, and pooled feacal samples and environmental samples were collected. Pathogenic Yersinia enterocolitica was found in 64% and Yersinia pseudotuberculosis in 45% of the sampled pig farms. All obtained isolates belonged to bioserotypes 4/O:3 and 2/O:3, respectively. Low biosecurity level was associated with a high prevalence of Y. enterocolitica on farms. Characterization with PFGE of 64 Y. enterocolitica and 27 Y. pseudotuberculosis isolates revealed seven and two different genotypes, respectively. Dominant enteropathogenic Yersinia spp. genotypes were obtained in both pig feacal and carcase samples. The high contamination of pig carcases (25%) with enteropathogenic Yersinia spp. may be an important factor contributing to the high incidence of human yersiniosis in Lithuania.

  19. Relationship between food deprivation before transport and aggression in pigs held in lairage before slaughter.

    PubMed

    Brown, S N; Knowles, T G; Edwards, J E; Warriss, P D

    1999-11-27

    Pigs from three farms were deprived of food for up to one hour, 12 hours or 18 hours before being sent for slaughter. In lairage, the animals' behaviour was monitored, and at slaughter a blood sample was collected and analysed for cortisol, lactate and creatine phosphokinase, potential indicators of stress and physical activity. The carcases were assessed for skin damage as an index of fighting, and rigor in the hind leg as an indicator of stress and/or fatigue. Measurements were also made of cold carcase weight, backfat thickness and liver glycogen concentration. General activity was very high on entry to the lairage pen. Drinking and mounting occurred almost immediately. Fighting developed after an exploratory period, and could last up to 60 minutes. There were large differences in the behaviour of pigs from the three farms. Pigs from farm A fought frequently but showed little mounting activity, whereas pigs from farm C were involved in mounting but little fighting. The period of food deprivation had no effect on average skin damage or rigor score, but the frequency of carcases with the highest scores was different The pigs deprived of food for up to an hour had the lowest incidence of severe skin damage and high rigor scores. Boars had a higher incidence of severe skin damage but a lower incidence of carcases with a high rigor score than gilts. Liver glycogen was almost completely depleted in the pigs deprived of food for 12 and 18 hours and was lower in the pigs deprived for up to an hour than in animals fed immediately before slaughter. The period of food deprivation had no effect on the levels of cortisol, creatine phosphokinase or lactate in the blood.

  20. Toxoplasma gondii infection in slaughter pigs in Serbia: seroprevalence and demonstration of parasites in blood

    PubMed Central

    2011-01-01

    A seroepizootiological study of Toxoplasma gondii infection involving a total of 488 slaughter pigs (468 market-weight pigs and 20 sows) in the Belgrade area, also included examination of the presence of T. gondii in the blood. Blood sampled at the slaughter line was examined for specific antibodies by modified direct agglutination, and blood clots of those seropositive at titres of 1:50-1:12800 were bioassayed in mice. The overall seroprevalence was 9.2%, significantly higher (p = 0.0063) in sows (30.0%) than in market-weight pigs (8.3%). Amongst the 22 bioassays performed, a total of 16 (72.7%) were positive, by observation of T. gondii cysts (12), seropositivity (7, including 3 in which cysts were not detected), and/or detection of T. gondii DNA by real-time PCR (12, including one otherwise negative). The positive bioassays originated from the blood of 12 market-weight pigs and 4 sows. Despite a general increase in the rate of demonstration of T. gondii with the increase in the specific antibody level, the association was not significant (p = 0.101). The risk of infection was 41-fold increased in sows vs market-weight pigs, and 15-fold in pigs from smallholders' finishing type farms vs those from large farrow-to-finish farms. The presence of viable T. gondii in a proportion of the samples indicates that some of the pigs had an active parasitaemia at the time of slaughter, which, along with the seroprevalence established, points to a potential source of human infection in Serbia. This is the first report on parasitaemia in naturally infected swine. PMID:21314900

  1. Prevalence of Pathogenic Yersinia enterocolitica in Finnish Slaughter Pigs.

    PubMed

    Rahikainen Ibañez, T; Laukkanen-Ninios, R; Hakkinen, M; Johansson, T; Vilar, M; Korkeala, H

    2016-04-01

    The prevalence of human pathogenic Yersinia enterocolitica was determined in tonsil and intestinal content samples from 388 healthy fattening pigs at the four biggest Finnish slaughterhouses. These slaughterhouses process 73% of pigs in Finland. Tonsil samples were tested by PCR targeted for yadA, and intestinal samples were cultured. All pathogenic Y. enterocolitica isolates represented bioserotype 4/O:3. The prevalence of Y. enterocolitica in tonsil samples was 60% (95% confidence limit, 55.4 to 65.1%), and its prevalence in intestinal samples was 26% (95% confidence limit, 22.1 to 31.2%). The prevalence of Y. enterocolitica in tonsil and intestinal samples varied between the four slaughterhouses. The tonsil prevalence of Y. enterocolitica was higher in slaughterhouse B, and the prevalence in intestinal content was higher in slaughterhouse C. There were more positive results in both tonsil and intestinal samples in pigs coming from fattening farms than in pigs coming from farrowing-and-fattening farms. A seasonal variation was observed in the prevalence of Y. enterocolitica in intestinal samples, with the highest prevalence during July and August, but no seasonal variation was detected in tonsil samples.

  2. Staphylococcus aureus ST398 from slaughter pigs in northeast China.

    PubMed

    Yan, Xiaomei; Yu, Xiaojie; Tao, Xiaoxia; Zhang, Jianfeng; Zhang, Binghua; Dong, Rui; Xue, Chengyu; Grundmann, Hajo; Zhang, Jianzhong

    2014-05-01

    To describe the prevalence and population structure of Staphylococcus aureus bacteria that colonize pigs at slaughterhouses in northeastern China, nose swabs were collected from pigs in two slaughterhouses in Harbin, Heilongjiang Province, China in 2009. S. aureus isolates were characterized by multilocus sequence typing (MLST), spa typing, SCCmec typing, antimicrobial susceptibility testing and pvl gene detection. A total of 200 S. aureus isolates were collected from 590 pigs (33.9%, 200/590), of which 162 (81%, 162/200) were methicillin-susceptible S. aureus (MSSA) and 38 (19%, 38/200) were methicillin-resistant S. aureus (MRSA). Ninety-nine of the MSSA isolates (99/162, 61.1%) were ST398, which represented the dominant sequence type overall. Eighty-seven isolates were ST9 (87/200, 43.5%), and all MRSA belonged to that sequence type which consisted of the spa types t899 and t2922. Among the MSSA strains, t034, t899 and t4358 were the most dominant spa types (139/162, 85.8%). All MRSA isolates harbored SCCmec type IVb. The pvl gene was only detected in 3 ST7/t2119 MSSA isolates. All MRSA but more importantly also 82.7% (134/162) of the MSSA isolates were resistant to six or more antibiotics. Moreover, a novel resistance determinant-lsa(E) was identified among 22% (44/200) of all isolates. In conclusion, pigs in northeast China are frequently colonized with ST398 MSSA. MRSA with this sequence type, typically associated with pigs in Europe, was not found. High levels of multiple antibiotic resistance among MRSA isolates as well as MSSA isolates are a public health concern.

  3. Prevalence, capsular type and antimicrobial susceptibility of Streptococcus suis isolated from slaughter pigs in Korea.

    PubMed Central

    Han, D U; Choi, C; Ham, H J; Jung, J H; Cho, W S; Kim, J; Higgins, R; Chae, C

    2001-01-01

    This study was undertaken to determine the prevalence, capsular serotype, and antimicrobial susceptibility of Streptococcus suis isolated from slaughter pigs. Capsular serotype and antimicrobial susceptibility were determined by coagglutination test and agar dilution minimum inhibitory concentration, respectively. Streptococcus suis was isolated from 55 of the 406 palatine tonsillar samples tested (13.8%) and 14 of the 29 sampled herds (48.3%). Of the 55 isolates recovered from slaughter pigs, 26 (47.3%) were untypeable. Of the remaining 29 isolates, capsular serotypes 9 (9 isolates) and 16 (4 isolates) were the most common, followed by capsular serotypes 4 (3 isolates) and 7 (3 isolates). Every capsulated isolate was typeable and no palatine tonsillar sample yielded more than one serotype. Most of isolates were susceptible to low concentrations (MIC90) of amoxicillin (2 microg/mL), ceftiofur (1 microg/mL), and penicillin (1 microg/mL). No correlation was found between antimicrobial susceptibility and capsular serotype. PMID:11480519

  4. Seroprevalence of zoonotic parasites in pigs slaughtered in the Kathmandu Valley of Nepal.

    PubMed

    Devleesschauwer, Brecht; Pruvot, Mathieu; Joshi, Durga Datt; De Craeye, Stéphane; Jennes, Malgorzata; Ale, Anita; Welinski, Alma; Lama, Sanjyoti; Aryal, Arjun; Victor, Bjorn; Duchateau, Luc; Speybroeck, Niko; Vercruysse, Jozef; Dorny, Pierre

    2013-12-01

    For several years, the demand for pork has been on the rise in Nepal. To assess the importance of pork as a carrier of zoonotic agents, we performed a cross-sectional study in the Kathmandu Valley of Nepal, in which we serologically determined the infection status of slaughtered pigs with regard to three of the most important parasites transmitted through pork consumption: Trichinella spp., Taenia solium cysticerci, and Toxoplasma gondii. From 2007 to 2010, 742 pigs were sampled at slaughter, of which 0.1% (95% confidence interval [CI] 0.0-0.7%) were found positive for Trichinella infection, 13.8% (95% credibility interval [CrI] 0.8-28.5%) for T. solium cysticercosis, and 11.7% (95% CI 5.2-17.5%) for Toxoplasma infection. Further monitoring of the related animal and human disease burden and strengthening of food safety protocols throughout the pork production chain are strongly recommended.

  5. Pleuritis in slaughter pigs: relations between lung lesions and bacteriology in 10 herds with high pleuritis.

    PubMed

    Jirawattanapong, Pichai; Stockhofe-Zurwieden, Norbert; van Leengoed, Leo; Wisselink, Henk; Raymakers, Rudolf; Cruijsen, Toine; van der Peet-Schwering, Carola; Nielen, Mirjam; van Nes, Arie

    2010-02-01

    Pleuritis in slaughter pigs has increased in recent years in the Netherlands. The aim of the present study was to determine what respiratory pathogens were involved in pleuritis. In total, lungs of 968 slaughter pigs from 10 herds with high prevalence of pleuritis were morphologically examined for size, location, and type of lesions. Moreover, histology and bacteriology were performed. Examination of gross lung lesions showed 45% pleuritis, 14% pleuropneumonia and 38% catarrhal pneumonia. Peribronchiolar cuffing was found in 61 of 142 samples. Actinobacillus pleuropneumoniae was cultured from 22 lung samples from four herds. Pasteurella multocida was cultured from 55 lung samples in eight herds. No specific pattern with respect to the causal pathogens was found. In conclusion, no single infectious cause of pleuritis was found. A variety of infectious agents combined with environmental factors should be considered as a cause of pleuritis.

  6. Welfare measurements of finishing pigs on the day of slaughter: a review.

    PubMed

    Brandt, Pia; Aaslyng, Margit Dall

    2015-05-01

    Animal welfare on the day of slaughter is of increasing concern to the authorities and consumers alike, creating a need not only to optimize the welfare of the animals but also to document the level of welfare. The day of slaughter is composed of a variety of stages, initiated when the pigs leave the home pen and including pick-up facilities, transport, lairage, stunning and sticking. At each of these stages, the animals are exposed to different stressors that, both individually and in interaction with one another, can compromise welfare. As part of the initial work aiming to document the welfare of finishing pigs on the day of slaughter, this paper provides an overview of the individual stages including a discussion of potential stressors and potential welfare measurements. These measurements are discussed with regard to their relevance and suitability for documentation of animal welfare on the day of slaughter for development of on-site tools for continuous automatic monitoring of animal welfare.

  7. Mycobacterium bovis infections in slaughter pigs in Mubende district, Uganda: a public health concern

    PubMed Central

    2012-01-01

    Background Bovine tuberculosis (TB) caused by Mycobacterium bovis is primarily a disease of ruminants, particularly cattle (Bos primigenius) and buffalo (Syncerus caffer), and is endemic in most developing countries. To date, studies done in Uganda have documented the prevalence of M. bovis in cattle, humans and wild life, in addition to non-tuberculous mycobacteria in pigs. Pigs are increasingly becoming an important component of the livestock sector and share the human ecosystem in rural Uganda. It is therefore of public health interest that they are not a source of human infections. As a follow up to previously published findings on mycobacteria in pigs, this study was aimed at investigating the occurrence and molecular characteristics of M. bovis detected in slaughter pigs in Mubende district, Uganda. One hundred fifty mesenteric lymph nodes with lesions suggestive of mycobacterial infections were collected from approximately one thousand slaughtered pigs in Mubende district over a period of five months. The isolation and identification of M. bovis was done using conventional mycobacteriological methods. Mycobacteria belonging to the Mycobacterium tuberculosis complex (MTC) were identified to species level using deletion analysis. Molecular typing was done using Spoligotyping and MIRU-VNTR analysis. Molecular data were analysed and interpreted using MIRU-VNTR plus, SpolDB4.0 and the Mycobacterium bovis spoligo database. Results Of the examined animals, one boar and two sows from Madudu Sub County were infected with M. bovis which presented as lesions of a deep yellow colour and a grit-like texture in the mesenteric lymph nodes. This represents 2% (3/150) of the lymph nodes where lesions suggestive of mycobacterial infections were detected. Molecular analysis revealed that the isolates from the infected pigs showed identical MIRU-VNTR profile and spoligotype (SB1469). Conclusions This is the first study documenting the occurrence of M. bovis in slaughter pigs in

  8. Possible impact of the "yellow card" antimicrobial scheme on meat inspection lesions in Danish finisher pigs.

    PubMed

    Alban, L; Dahl, J; Andreasen, M; Petersen, J V; Sandberg, M

    2013-03-01

    In 2010, the "yellow card scheme" which was adopted by the Danish Veterinary and Food Administration imposed restrictions on pig farmers who used more antimicrobials than twice the average. To study the potential impact on pig health, we looked into antimicrobial consumption and vaccine use data from the monitoring programme Vetstat, covering all treatments conducted on Danish pigs between January 2010 and July 2011. The decrease in antimicrobial consumption was pronounced for all age groups (sows/piglets, weaners and finishers) treated for either gastro-intestinal or respiratory disease. Evaluated over 12 months, use of vaccines increased in general: PCV2-related infections (+31%), gastro-intestinal disease (27%), respiratory infections (21%) whereas use of vaccines against other infections remained almost constant (-18%). Data from meat inspection of finisher pigs from before and after introduction of the scheme were compared. This included 1.7 million finisher pigs originating from 2765 pig farms, slaughtered on one large Danish abattoir and covered the first 9 weeks in 2010 and 2011, respectively. Nine lesions of chronic nature and infectious origin and the code "condemned" were selected. The prevalence of these lesions was calculated. Logistic regression models with year and week as explanatory variables were used to identify whether the prevalence of a lesion changed from 2010 to 2011. Possible clustering due to correlation within herds and between weekly shipments of animals originating from the same herd was taken into account in the models. The most common lesion seen was chronic pleuritis (∼23%) while the other lesions occurred less-commonly (<1%). For osteomyelitis, pleuritis, chronic arthritis and condemnation, no differences were observed between the 2 years. The prevalence of chronic peritonitis (OR=1.5), umbilical hernia (OR=1.2) and chronic enteritis (OR=1.2) were statistically higher in 2011 compared to 2010, whereas it was lower for tail bite

  9. Impact of the Salmonella status of market-age pigs and the pre-slaughter process on Salmonella caecal contamination at slaughter.

    PubMed

    Beloeil, Pierre-Alexandre; Chauvin, Claire; Proux, Karine; Madec, François; Fravalo, Philippe; Alioum, Ahmadou

    2004-01-01

    The aim of this study was to evaluate the impact of the pre-slaughter process on Salmonella caecal contamination of pigs at slaughter. An observational study was carried out in 2001 on 101 conventional farrow-to-finish pig farms. On each farm, one batch of contemporary pigs was followed from the end of the fattening period until slaughter. The Salmonella bacteriological status of the batches was assessed by environmental samples of faecal material. The serological Salmonella status was obtained on 30 individually identified market-age pigs using an indirect ELISA test. At the slaughterhouse, 25 g of caecal contents were taken from 10 of the identified pigs. Faecal and caecal material were analysed according to a classical bacteriological method. A questionnaire was designed to obtain information about the type of feeding during the fattening period (dry versus wet), the duration of fasting on the farm before leaving for the slaughterhouse, the duration of transport between the farm and the slaughterhouse, the holding time in lairage at the slaughterhouse and loading and unloading conditions on the farm and at the slaughterhouse. To assess the relationships between these factors and the Salmonella caecal status of the pigs and the batches, two logistic models were fitted at the individual and at the batch level, respectively. The first analysis was performed using a random effects logistic regression model. The second analysis was based on a cumulative logit model with a positive caecal rate classified into three classes as the outcome variable. The results showed that the Salmonella status of market-age pigs assessed on the farm either by serological or bacteriological examinations and the time spent in lairage before slaughtering played a crucial role on caecal contamination. In the light of these results, actions should be considered both on the farm and at the slaughterhouse to decrease the risk of Salmonella contamination of the caecal contents.

  10. Taenia hydatigena cysticercosis in slaughtered pigs, goats, and sheep in Tanzania.

    PubMed

    Braae, Uffe Christian; Kabululu, Mwemezi; Nørmark, Michelle Elisabeth; Nejsum, Peter; Ngowi, Helena Aminel; Johansen, Maria Vang

    2015-12-01

    Few studies have been carried out in Africa to estimate the prevalence of Taenia hydatigena. With the aim to determine the prevalence of T. hydatigena in slaughtered pigs and small ruminants (goats and sheep) in Mbeya, Tanzania, two cross-sectional surveys were carried out investigating pigs in April to May 2014 and small ruminants in September 2012. In total, 243 pigs were examined post-mortem for T. hydatigena cysts which were found in 16 (6.6 %) pigs. The majority (80 %) of cysts were found on the omentum and the rest on the liver (20 %), all on the visceral surface. Two pigs were also found infected with Taenia solium but showed no signs of other infections. A total of 392 goats and 27 sheep were examined post-mortem, and the prevalence of T. hydatigena was similar in goats and sheep with 45.7 and 51.9 %, respectively. DNA sequencing of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1) from a subsample of metacestodes from goats and sheep confirmed the T. hydatigena infection. The prevalence found in small ruminants was comparable to other studies conducted in Africa, but for pigs, it is one of the highest recorded to date. The present study also confirms the occurrence of T. hydatigena and T. solium in pigs from Mbeya. Further studies are needed to determine the impact of T. hydatigena on production under sub-Saharan conditions and the financial consequences for smallholder farmers.

  11. Prevalence of infection with Toxoplasma gondii in landrace and mixed breed pigs slaughtered in Baja California Sur state, Mexico

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Purpose We performed a cross-sectional study to determine the seroprevalence of Toxoplasma gondii infection in 308 domestic pigs slaughtered in La Paz, Baja California Sur State, Mexico using the modified agglutination test (MAT, cut off 1:25). Results Forty (13%) of the 308 pigs were seropositive ...

  12. Investigation of Salmonella enterica in Sardinian slaughter pigs: prevalence, serotype and genotype characterization.

    PubMed

    Piras, Francesca; Brown, Derek J; Meloni, Domenico; Mureddu, Anna; Mazzette, Rina

    2011-12-02

    In order to improve the knowledge about the presence of Salmonella in pork meat in Sardinia (Italy), the prevalence and the sources of Salmonella at 5 pig slaughterhouses (slaughtered pigs and environment) were investigated and the isolates were characterised. A total of 462 samples were collected, 425 from pigs at slaughter and 41 from the slaughterhouse environment. Salmonella was isolated from 26/85 (30.5%) mesenteric lymph nodes, 14/85 (16.4%) colon contents, and from 12/85 (14.1%) carcasses and livers. Salmonella prevalence was 38% (8/21) in samples from surfaces not in contact with meat, and 35% (7/20) in those from surfaces in contact with meat. Thirty-one pigs were identified as carriers of Salmonella in lymph nodes and/or colon content, but of these, only 8 carcasses were positive. A total of 103 Salmonella isolates were serotyped and genotyped. Eight different serotypes were detected; the most common were S. Derby (44/103, 42.7%) and S. Typhimurium (24/103, 23.3%). The most prevalent S. Typhimurium phage type was DT193. Thirty-two isolates were found to be resistant to more than one antimicrobial (MDR). Pulse-field gel electrophoresis (PFGE) permitted the resolution of XbaI macrorestriction fragments of the Salmonella strains into 20 distinct pulsotypes. Combined application of a plasmid profiling assay (PPA) and PFGE gave useful additional information to assist in tracing the routes of Salmonella contamination in abattoirs. To reduce Salmonella prevalence some preventive measures should be encouraged: the origin of infected slaughter animals should be identified and direct and cross-contamination of carcasses should be avoided by adhering to HACCP principles in association with good hygiene procedures (GHP).

  13. Continuous straw provision reduces prevalence of oesophago-gastric ulcer in pigs slaughtered at 170 kg (heavy pigs).

    PubMed

    Di Martino, Guido; Capello, Katia; Scollo, Annalisa; Gottardo, Flaviana; Stefani, Anna Lisa; Rampin, Fabio; Schiavon, Eliana; Marangon, Stefano; Bonfanti, Lebana

    2013-12-01

    Adopting a 2 × 2 × 2 factorial design, this study evaluated whether continuous straw provision by racks, tail docking and gender (barrows vs. females) have an effect on the prevalence of lung lesions and oesophago-gastric ulcer (OGU) visually scored at slaughter in 635 Italian heavy pigs (169 ± 4 kg). The lung lesions were very low (72% of pigs with score 0), and were not significantly different among the experimental groups. Overall, OGU was diagnosed in 47% of the pigs. The consumption of small amounts of straw (70 g/day/pig) represented a protective factor against the onset of OGU (OR: 0.27). Barrows were more likely than females to have OGU (OR: 1.52), while no significant differences between docked and undocked pigs were detected. Nevertheless, the presence of straw acted as a protective factor particularly in undocked pigs (OR: 0.16), suggesting that in this group the absence of rooting material may have a stronger effect on welfare.

  14. The impact of gastrointestinal parasites infection on slaughter efficiency in pigs.

    PubMed

    Knecht, Damian; Jankowska, Anna; Zaleśny, Grzegorz

    2012-03-23

    The aim of the present study was to determine an influence of parasites invasion on fodder consumption and slaughter yield of fatteners in three different maintenance systems. The experimental part of the study was conducted on pigs farm producing in a close cycle. The study on internal parasites of fatteners were conducted based on coproscopic methods. In order to describe the relationship between following variables: meatiness, slaughter yield, fodder consumption and mean EPG value, the principal component analysis (PCA) was used. The analysis between fodder consumption and maintenance system and fodder consumption and helminths infection did not demonstrate any significant relationship. The analysis between slaughter yield and meatiness and an infection demonstrated in turn a decrease in both parameters values in the two maintenance systems, i.e. in fatteners kept on litter meatiness decrease in infected fatteners of 4.2% and yield of 1.7%. On slatted floor meatiness decrease in infected fatteners of 6.1% and yield of 2.7%. The decreasing tendency in meat content (3.7%) and in slaughter yield (1.1%) was also observed in fatteners maintained on deep litter, however the values were not significant statistically.

  15. Occurrence, Characterization, and Antimicrobial Susceptibility of Salmonella enterica in Slaughtered Pigs in Sardinia.

    PubMed

    Fois, Federica; Piras, Francesca; Torpdahl, Mia; Mazza, Roberta; Consolati, Simonetta G; Spanu, Carlo; Scarano, Christian; De Santis, Enrico P L

    2017-02-22

    The aim of this study was to determine Salmonella occurrence in slaughtered finishing pigs and piglets and in slaughterhouse environment in order to characterize the isolates with phenotypical (antimicrobial testing) and molecular (PFGE, MLVA) methods. Nine slaughterhouses located in Sardinia were visited. Six hundred and eight samples collected from 106 pigs and 108 environmental samples were collected and analyzed. Salmonella was isolated in 65 of 504 (12.9%) samples from finishing pigs, with an occurrence of 15.1% in colon content, 12.7% in lymph nodes and liver, and 11.1% in carcass surface samples. Salmonella was never detected in piglets. The combined results of serotyping and PFGE showed a possible self-contamination in 71.5% of Salmonella positive carcasses of lymph nodes and/or colon content carriers, pointing out the role of healthy pigs for carcass contamination. A significantly higher (P < 0.05) occurrence was detected in finishing pigs of EC countries origin (23%) than in pigs of local farms (8%). Salmonella was also detected in 3.7% of environmental samples. The most prevalent serovar was S. Anatum, followed by S. Rissen, S. Derby, and monophasic S. Typhimurium. Resistance to at least 3 antimicrobial was observed in 97.1% of strains and 7 different patterns of multiple resistance were identified. The most common resistance was detected against sulphonamide compounds. A strict slaughterhouse application of hygiene standards is essential to control the risk of Salmonella contamination.

  16. Supra-nutritional vitamin E supplementation for 28 days before slaughter maximises muscle vitamin E concentration in finisher pigs.

    PubMed

    Kim, J C; Jose, C G; Trezona, M; Moore, K L; Pluske, J R; Mullan, B P

    2015-12-01

    A 4 × 3 factorial experiment (n=8 pigs per treatment combination) was conducted with 96 female Landrace × Large White pigs to examine the required level of dietary vitamin E and optimum feeding duration before slaughter to maximise muscle vitamin E content in the Longissimus thoracis et lumborum (LTL) muscle. The respective factors were four dietary levels of vitamin E (supplemented as dl-α-tocopheryl acetate; 35, 300, 500, and 700 IU/kg) and three feeding durations (14, 28 and 42 days before slaughter). Vitamin E concentration in the LTL was maximised at 6 mg/kg, which was achieved by feeding a 700 IU vitamin E diet for 28 days before slaughter (P<0.001). There was no further increase in the vitamin E content of the LTL by feeding the high vitamin E diet more than 28 days before slaughter.

  17. Campylobacter in healthy slaughter pigs: a possible source of infection for man.

    PubMed

    Sticht-Groh, V

    1982-01-30

    Campylobacter were isolated from 103 of 173 (59 per cent) specimens of healthy slaughter pig faeces, washed intestines and water samples collected from a slaughterhouse and butcher's shop in West Germany. As most cases of human campylobacter enteritis are caused by Campylobacter jejuni, an attempt was made to find this organism among the isolates. Twenty-five out of the 103 strains (24 per cent) were identified as C jejuni. C jejuni was also isolated from salted water samples after overnight bowel storage in the butcher's shop, indicating that the customary salt preparation of the intestines did not eliminate all organisms present.

  18. Isolation of acriflavine resistant Erysipelothrix rhusiopathiae from slaughter pigs in Japan.

    PubMed

    Makino, S; Ishizaki, H; Shirahata, T; Fujiwara, S; Sawada, T

    1998-09-01

    Erysipelothrix rhusiopathiae is the causative agent of swine erysipelas. Although an attenuated vaccine is used in Japan, recent increases in disease occurrence have cast doubts on its efficacy. We investigated the similarity between the vaccine strain and E. rhusiopathiae field isolates by the analysis of acriflavine resistance (the vaccine strain marker), serotype, DNA fingerprinting and pathogenicity to mice. Although 7 acriflavine resistant E. rhusiopathiae isolates were separated from arthritic lesions of slaughter pigs, we were unable to prove that they were identical to the vaccine strain.

  19. Prevalence of Hepatitis E Virus Infection in Pigs at the Time of Slaughter, United Kingdom, 2013.

    PubMed

    Grierson, Sylvia; Heaney, Judith; Cheney, Tanya; Morgan, Dilys; Wyllie, Stephen; Powell, Laura; Smith, Donald; Ijaz, Samreen; Steinbach, Falko; Choudhury, Bhudipa; Tedder, Richard S

    2015-08-01

    Since 2010, reports of infection with hepatitis E virus (HEV) have increased in England and Wales. Despite mounting evidence regarding the zoonotic potential of porcine HEV, there are limited data on its prevalence in pigs in the United Kingdom. We investigated antibody prevalence, active infection, and virus variation in serum and cecal content samples from 629 pigs at slaughter. Prevalence of antibodies to HEV was 92.8% (584/629), and HEV RNA was detected in 15% of cecal contents (93/629), 3% of plasma samples (22/629), and 2% of both (14/629). However, although HEV is prevalent in pigs in the United Kingdom and viremic pigs are entering the food chain, most (22/23) viral sequences clustered separately from the dominant type seen in humans. Thus, pigs raised in the United Kingdom are unlikely to be the main source of human HEV infections in the United Kingdom. Further research is needed to identify the source of these infections.

  20. Effects of pre-slaughter stressor and feeding preventative Chinese medicinal herbs on glycolysis and oxidative stability in pigs.

    PubMed

    Bai, Xiumei; Yan, Xue; Xie, Linqi; Hu, Xiaodong; Lin, Xi; Wu, Changzheng; Zhou, Ningcong; Wang, Anru; See, Miles Todd

    2016-08-01

    A total of 64 5-month-old Pietrain pigs were randomly allocated to four treatments with four replicates per treatment according to body weight. The pigs were fed either a standard corn-soybean meal based control diet (treatments 1 and 2), the standard diet with 1% Lycium barbarum (LB) (treatment 3), or the standard diet with 1% Polygala tenuifolia Willd (PT) (treatment 4). Serum lactic acid and glucose concentrations were increased in stressed pigs (P < 0.05). Addition of the herbs in the diet had no effect on the serum lactic acid concentration, but 1% LB decreased (P < 0.05) serum glucose concentration in the stressed pigs. Pre-slaughter stress also decreased (P < 0.01) liver glycogen concentration and the decrease could be inhibited by addition of 1% LB in the diet (P > 0.05). Pre-slaughter stress increased the concentration of maleic dialdehyde (MDA) (P < 0.05) and decreased glutathione peroxidase (GSH-Px) activity in serum, while dietary 1% LB increased (P < 0.05) the activity of GSH-Px and decreased the concentration of MDA in the serum. In conclusion, pre-slaughter stress induces oxidative stress in pigs and dietary supplementation with 1% LB improves antioxidant capacity in stressed pigs before slaughtering.

  1. Prevalence of infection with Toxoplasma gondii in landrace and mixed breed pigs slaughtered in Baja California Sur State, Mexico.

    PubMed

    Alvarado-Esquivel, Cosme; Vazquez-Morales, Renata Fabiola; Colado-Romero, Edgar Eusebio; Guzmán-Sánchez, Ramiro; Liesenfeld, Oliver; Dubey, Jitender P

    2015-03-01

    We performed a cross-sectional study to determine the seroprevalence of Toxoplasma gondii infection in 308 domestic pigs slaughtered in La Paz, Baja California Sur State, Mexico using the modified agglutination test (MAT, cut off 1:25). Forty (13%) of the 308 pigs were seropositive with MAT titers of 1:25 in 16, 1:50 in 5, 1:100 in 4, 1:200 in 5, 1:400 in 3, 1:800 in 3, 1:1600 in 2, and 1:3200 in 2. Multivariate analysis of pigs' characteristics showed that seropositivity to T. gondii was negatively associated with mixed breed (OR = 0.02; 95% CI: 0.003-0.26; P = 0.001). Other variables including sex, type of raising, and municipality did not show an association with T. gondii seropositivity by multivariate analysis. The frequency of high antibody titers (≥1:400) was significantly higher (P < 0.001) in Landrace pigs than mixed breed pigs. The seroprevalence of T. gondii infection in pigs for slaughter in Baja California Sur State is low compared with seroprevalences reported in pigs in other Mexican states. Landrace pigs demonstrated higher seroprevalence rates and antibody levels than mixed breed pigs. This is the first report of T. gondii infection in pigs raised in a desert climate.

  2. Serum acute phase proteins as biomarkers of pleuritis and cranio-ventral pulmonary consolidation in slaughter-aged pigs.

    PubMed

    Saco, Yolanda; Fraile, Lorenzo; Giménez, Mercè; Alegre, Ana; López-Jimenez, Rosa; Cortey, Martí; Segalés, Joaquim; Bassols, Anna

    2011-08-01

    The purpose of this study was to investigate the relationship between the existence of lung lesions in pigs at slaughter and the concentration of the serum acute phase proteins (APP), haptoglobin (Hp), pig-major acute protein (Pig-MAP) and C-reactive protein (CRP). A total of 24 pig farms were selected out of a larger farm database previously screened to study risk factors associated with pleuritis and cranio-ventral pulmonary consolidation (CVPC) lesions at slaughter-aged pigs in Spain. The farms were classified as "pleuritis negative (P-) or positive (P+)" and as "CVPC negative (M-) or positive (M+)" and divided into four groups according to a 2X2 factorial design (P-M-, P-M+, P+M-, P+M+). Also at slaughter, blood from 20 randomly selected pigs from each farm was collected. Obtained serum samples were used to measure acute phase proteins. All APP concentrations were significantly higher for M+ farms than for M- ones. However, only Hp and Pig-MAP showed significantly higher concentrations for P+ farms than for P- ones. Pig-MAP was the most sensitive biomarker since it was able to clearly discriminate between P-/P+ and M-/M+ groups (p<0.001 in both cases). Hp was an excellent marker for pleuritis and good for CVPC lesions. CRP was able to discriminate for CVPC lesions but not for pleuritis. The present results indicate that Pig-MAP and, possibly Hp, may be used as potential markers to characterise and discriminate respiratory lesions in swine herds at slaughter.

  3. Prevalence of extra-intestinal porcine helminth infections and assessment of sanitary conditions of pig slaughter slabs in Dar es Salaam city, Tanzania.

    PubMed

    Mkupasi, Ernatus M; Ngowi, Helena A; Nonga, Hezron Emmanuel

    2011-02-01

    A study was carried out to establish the prevalence of extra-intestinal porcine helminth infections and to assess the pig slaughter slab sanitary conditions in Dar es Salaam city, Tanzania. A total of 24 privately owned pig slaughter slabs were assessed. All slaughter slabs were sub-standard; wrongly located, poorly designed and constructed and lacked most basic requirements for a slaughter house. Because of inadequate slaughtering, disposal and cleaning facilities, the slaughter slabs were under unhygienic condition with questionable safety, soundness and wholesomeness of the pork produced. Routine meat inspection procedures were used to detect extra-intestinal porcine helminth infections. Of the 731 examined pigs; 8.1%, 5.9% and 0.4% were infected with ascariosis, porcine cysticercosis and hydatidosis, respectively. It was noted that almost all slaughter pigs in Dar es Salaam originated from different regions. Based on the region of origin, the status of porcine cysticercosis was 8.2% for Dodoma (n = 98), 8.2% for Manyara (n = 260) and 6.9% for Mbeya (n = 116). This study disclosed the unhygienic sanitary condition prevailing in Dar es Salaam pig slaughter slabs and recommends that strategies should be devised to improve the situation. Porcine ascariosis and cysticercosis were widely prevalent and caused economic losses due to condemnations. Because of their zoonotic nature, the observed extra-intestinal porcine helminth infections in pig pose a public health risk among consumers. Thus, there is a need to introduce appropriate control measures of parasitic infections in pigs.

  4. Methicillin-resistant Staphylococcus aureus (MRSA) in slaughtered pigs and abattoir workers in Italy.

    PubMed

    Normanno, Giovanni; Dambrosio, Angela; Lorusso, Vanessa; Samoilis, Georgios; Di Taranto, Pietro; Parisi, Antonio

    2015-10-01

    Methicillin-resistant Staphylococcus aureus (MRSA) is a pathogen present in the hospital environment (HA-MRSA), in the community (CA-MRSA) and in livestock, including pigs (LA-MRSA). MRSA may enter the human food chain during slaughtering and may infect humans coming into direct contact with pigs or pork products. This study aimed to determine the prevalence and characteristics of MRSA isolated from pigs and workers at industrial abattoirs in southern Italy. A total of 215 pig nasal swabs were screened for the presence of MRSA using PCR. An MRSA isolate was detected from each mecA/nuc PCR-positive sample and characterized by spa-typing, Multi-Locus Sequence Typing, SCC-mec and Panton-Valentine Leukocidin (PVL), and also tested for the production of staphylococcal enterotoxins (SEs). Eighty-one MRSA isolates (37.6%) were obtained from the 215 pig nasal swabs; 37 of these isolates were further characterized, and showed 18 different spa-types and 8 different STs. The most frequently recovered STs were ST398 (CC398-t034, t011, t899, t1939 - 43.2%) followed by ST8 (CC8-t008, t064, t2953, t5270 - 24.3%) and ST1 (CC1-t127, t174, t2207 - 10.8%). Nine MRSA isolates were obtained from the 113 human swabs; the isolates showed 5 different spa-types and 5 different STs, including the novel ST2794 (t159). The most representative STs recovered were ST1 (CC1-t127) and ST398 (CC398-t034) (33.3%). None of the MRSA isolates showed the ability to produce SEs and PVL and all resulted resistant to two or more classes of antimicrobials. This study shows the great genetic diversity of MRSA strains in slaughtered pigs and in abattoir employees in Italy, and clearly demonstrates the need for improved hygiene standards to reduce the risk of occupational and food-borne infection linked to the handling/consumption of raw pork containing MRSA.

  5. Noninfectious factors associated with pneumonia and pleuritis in slaughtered pigs from 143 farrow-to-finish pig farms.

    PubMed

    Fablet, C; Dorenlor, V; Eono, F; Eveno, E; Jolly, J P; Portier, F; Bidan, F; Madec, F; Rose, N

    2012-05-01

    A cross-sectional study involving 143 farrow-to-finish herds was carried out to identify herd-level noninfectious factors associated with pneumonia and pleuritis in slaughter pigs. Data related to herd characteristics, biosecurity, management and housing conditions were collected by questionnaire during a farm visit. Climatic conditions were measured over 20 h in the post-weaning and finishing rooms where the slaughter pigs were kept. After these on-farm investigations, the finishing pigs were examined at slaughter for lung lesions. A sample of 30 randomly selected pigs per herd was scored for pneumonia and pleuritis. Herds were grouped into three categories according to their pneumonia median score (class 1: ≤ 0.5; class 2: 0.53.75). For pleuritis, a herd was deemed affected if at least one pig had a high pleuritis score (≥ 3). A multinomial logistic regression model was used to identify factors associated with pneumonia classes 2 and 3. A logistic regression for binary outcome was used to identify risk factors for severe pleuritis. An interval of less than four weeks between successive batches (OR=4.5, 95% confidence interval (95% CI): 1.5-13.6, p<0.01), large finishing room size (OR=4.3, 95% CI: 1.6-11.6, p<0.01) and high mean CO(2) concentration in the finishing room (OR=4.2, 95%CI: 1.6-11.3, p<0.01), significantly increased the odds for a herd to be in class 2 for pneumonia. The same risk factors were found for class 3 and, in addition, a direct fresh air inlet from outside or from the corridor in the post-weaning room vs an appropriate ceiling above the pigs (OR=5.1, 95% CI: 1.4-18.8, p=0.01). The risk for a herd to have at least one pig with a high pleuritis score was increased when the farrowing facilities were not disinsected (OR=2.7, 95% CI: 1.2-5.8, p=0.01), when tail docking was performed later than 1.5 days after birth (OR=2.6, 95% CI: 1.2-5.7, p=0.01) and if the piglets were castrated when more than 14 days old (OR=2

  6. Phylogenetic analysis of Hepatitis E virus strains isolated from slaughter-age pigs in Colombia.

    PubMed

    Forero, Jorge E; Gutiérrez-Vergara, Cristian; Parra Suescún, Jaime; Correa, Guillermo; Rodríguez, Berardo; Gutiérrez, Lina A; Díaz, Francisco J; López-Herrera, Albeiro

    2017-04-01

    Hepatitis E virus (HEV) is a zoonotic virus that causes acute hepatitis in humans, and can be transmitted via the fecal-oral route. Pigs are considered to be a reservoir for this infection-mainly where the disease is not endemic. In a previous study conducted in Antioquia, which is a region in Colombia where the production and consumption of pork meat is higher than in the rest of the country, the presence of anti-HEV IgG-type antibodies was reported in slaughter-age pigs. Aiming to identify the HEV genotype circulating in swine, animal liver, and feces samples from five swine cattle slaughterhouses located in six different sub-regions of Antioquia were collected. A nested RT-PCR (nRT-PCR) was used in order to amplify the HEV ORF-1 (170bp) and ORF-2 (348, and 958bp). The amplicons yielded in this study were sequenced, and a molecular phylogeny analysis based on the maximum likelihood method, including HEV sequences reported in several countries, was performed. Phylogeny analysis revealed that HEV amplification fragments from Antioquia's pigs were grouped in three clades within the sub-genotype 3a without a specific geographical structure, and were also genetically related to Japanese and American HEV sequences. This analysis provides the first approach on the genetic diversity and circulation dynamics of HEV in Colombian herds.

  7. Prevalence of hepatitis E virus in slaughter-age pigs in Scotland.

    PubMed

    Crossan, C; Grierson, S; Thomson, J; Ward, A; Nunez-Garcia, J; Banks, M; Scobie, L

    2015-07-01

    The prevalence of anti-HEV isotype-specific antibodies and viraemia were investigated in serum samples collected from slaughter-age pigs (aged 22-24 weeks) from 23 farms in Scotland. Of 176 serum samples tested, 29·0% (n = 51) were anti-HEV IgG positive, 36·9% (n = 65) anti-HEV IgA positive and 29·0% (n = 51) anti-HEV IgM positive. Overall seroprevalence (anti-HEV IgG+ and/or IgA+ and/or IgM+) was 61·4% (n = 108). HEV RNA was detected in 72/162 serum samples (44·4%). Partial sequence of ORF2 (98 nt) was obtained from eight HEV RNA-positive samples and phylogenetic analysis confirmed that they were all of genotype 3. This is the first report on the prevalence of HEV in pigs in Scotland. Given the increasing incidence of locally acquired HEV infection in the UK, evidence that HEV is a foodborne zoonosis emphasizes the need for surveillance in pigs.

  8. Changes in antimicrobial resistance in fecal bacteria associated with pig transit and holding times at slaughter plants.

    PubMed Central

    Molitoris, E; Fagerberg, D J; Quarles, C L; Krichevsky, M I

    1987-01-01

    Fecal coliforms, fecal streptococci, and antimicrobial resistance (AMR) associated with various pig transit and holding times were investigated at slaughter plants. Changes in the relative abundance of two biotypes of Streptococcus faecium were associated with transit and holding of pigs, although approximately 20% of the isolates were unidentified. The greatest variety of coliforms was isolated from porcine feces after short transit (2 h) or holding (3 h) times and was qualitatively similar to those from pigs on farms. Isolates from pigs with longer average transit or holding times were almost all Escherichia coli (four biotypes). Streptococcal resistance to most antimicrobial agents was significantly greater (P less than 0.05) in isolates from live pigs at slaughter plants than in those from pigs at farms and was apparent after a short transit time (2 h). Streptococci from pigs held an average of 15 h were less resistant to most antimicrobial agents than those from pigs held 3 or 43 h. When compared with short transit times, moderate transit times (6 h) were associated with significantly decreased (P less than 0.05) coliform resistance and decreased resistance transfer but a greater diversity of AMR patterns. Holding pigs overnight (14 h) was associated with lowered coliform resistance to several antimicrobial agents, compared with the resistance of isolates from pigs held 3 or 39 h. A substantial increase (18 to 48%) in the ability to transfer streptomycin resistance was demonstrated in coliforms from pigs held 39 h, when compared with those from pigs held 3 h.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:3606107

  9. Evaluation of the pathology, pathogenesis and aetiology of auricular elephantiasis in slaughter pigs.

    PubMed

    Kvist, P H; Jensen, E S; Aalbaek, B; Jensen, H E

    2002-12-01

    Ears from slaughter pigs with auricular elephantiasis (n = 24) and the corresponding lymph nodes (lnn.) (n = 26) were grossly, histopathologically and microbiologically examined. Immunostaining for IgM, IgG, Cd3epsilon and bacterial antigens of Arcanobacterium pyogenes and Staphylococcus aureus was performed by indirect enzyme-based techniques. Ears were variably thickened depending on the sampled area (basis, centre and apex). However, at all locations the thickness, the length from basis to apex and the weigh of whole ears with elephantiasis were significantly increased (P < 0.01). The corresponding lnn., that is, ln. parotideus superficialis and profundus, had also increased significantly (P < 0.01) in volume. Histopathologically, lesions of the ears and the corresponding lnn. revealed changes characterized by diffuse fibrosis intermingled with multiple pyogranulomatous foci containing asteroid bodies. In the majority of lesions, four distinct zones due to different cellular infiltrates encircled the central core of the asteroid bodies. In several lesions, the pyogranulomatous foci were contained within the lymph vessels. Immunohistochemically, only the bacterial antigen of S. aureus was detected within the cytoplasm of the macrophages and/or in the asteroid bodies of the ears (41.5%) and in the regional lnn. (30.8%). An abundant number of IgM, IgG and CD3epsilon-positive cells were present in all the pyogranulomatous lesions, whereas a positive IgG-staining was observed only in a single asteroid body. Thus, porcine auricular elephantiasis is a chronic pyogranulomatous inflammation that is frequently positive for S. aureus and is lymphogenically spread. Therefore, the lesions of the ears with auricular elephantiasis and the corresponding lnn. should be termed auricular botryomycosis and botryomycotic lymphadenitis, respectively. Moreover, as the disease is observed frequently in slaughter pigs it must also be considered according to the welfare of the animals

  10. Risk factors associated with pleuritis and cranio-ventral pulmonary consolidation in slaughter-aged pigs.

    PubMed

    Fraile, L; Alegre, A; López-Jiménez, R; Nofrarías, M; Segalés, J

    2010-06-01

    Examination of lung lesions at the slaughterhouse is a useful tool to estimate the importance of respiratory disease at farm, regional or national level. The objective of the present work was to describe the prevalence of gross lung lesions at slaughter, with a special focus on pleuritis and cranio-ventral pulmonary consolidation, and to identify major risk factors for these lesions. Data from 107 farms involving approximately 11,000 pigs enabled gross lung lesions to be correlated with serology to different swine respiratory pathogens as well as with production system characteristics and vaccination schedules. Pleuritis and cranio-ventral pulmonary consolidation lesions were recorded in 26.8% and 55.7% of slaughter-aged pigs, respectively. Among lungs with pleuritis, 50.1% had lesions compatible with Actinobacillus pleuropneumoniae (App) infection. Antibodies to porcine reproductive and respiratory syndrome (PRRSV), three subtypes (H1N1, H1N2 and H3N2) of swine influenza virus (SIV), App and Mycoplasma hyopneumoniae (Mhyo) were highly prevalent (>82%) in most of the farms. In a multivariable analysis, it was estimated (R(2)=0.40) that the percentage of animals with pleuritis compatible with App infection depended on the existence of an all in-all out by room management system and App and PRRSV herd seroprevalence. Moreover, it was possible to foresee (R(2)=0.59) that cranio-ventral pulmonary consolidation lesions (EP-like lesions) were affected by the type of farm ventilation, the presence of respiratory symptoms during the fattening period and Mhyo and SIV H1N2 herd seroprevalence.

  11. Effects of corn type and fasting time before slaughter on growth and plasma index in weaning pigs.

    PubMed

    Yang, C; He, J; Chen, D W; Yu, B; Yu, J; Mao, X B; Yang, K Y; Yuan, Z C

    2016-01-01

    A 2 × 2 factorial experiment was conducted to evaluate the effects of dietary corn type (waxy corn [WC] vs. nonwaxy corn [NC]) and fasting period (2 h vs. 12-16 h) before slaughter on growth and plasma index in weaning pigs. Twenty-four crossbred barrows (8.26 ± 0.47 kg) were allotted to 4 treatments with 6 replications of 1 pig per replicate metabolism cage. Waxy corn contained less fat, a lower amylase:amylopectin ratio, more CP, and more starch than NC. Pigs fed the WC diet had lower jejunum digesta pH compared with those fed the NC diet ( < 0.05). Maltase activity in the jejunum and ileum mucosa ( < 0.01), sucrose activity in the ileum mucosa ( < 0.01), and amylase activity in the pancreas and jejunum digesta ( < 0.05) were increased in pigs fed the WC diet relative to those fed the NC diet. But the total tract apparent digestibility (TTAD) of starch and CP was lower for the WC group ( < 0.05). Ingestion of the NC diet resulted in higher ( < 0.05) ADG and ADFI in the second week but did not affect ADG ( = 0.091) and the feed:gain ratio (F:G; = 0.077) during the whole experiment period. The plasma glucose ( < 0.01) concentration was higher and high-density lipoprotein cholesterol (HDL-C; < 0.01) concentrations was lower in the hepatic portal vein in pigs fed the WC diet relative to those fed the NC diet. Fasting 2 h before slaughter decreased the jejunum and ileum digesta pH compared with the 12-h fasting group ( < 0.01). Villus height increased in the duodenum ( < 0.01) and jejunum ( < 0.05) and the villus height:crypt depth ratio increased in the duodenum ( < 0.05) of pigs after shortening the fasting period before slaughter. Shortening the fasting time before slaughter resulted in higher plasma glucose ( < 0.05) concentrations and a higher HDL-C:low-density lipoprotein cholesterol (LDL-C) ratio ( < 0.05) whereas the LDL-C ( < 0.05) concentrations were reduced in the hepatic portal vein. The results of this experiment indicate that although the production

  12. Microbiomes of Unreactive and Pathologically Altered Ileocecal Lymph Nodes of Slaughter Pigs

    PubMed Central

    Mann, Evelyne; Dzieciol, Monika; Metzler-Zebeli, Barbara U.; Wagner, Martin

    2014-01-01

    Microbe-laden dendritic cells are shifted to ileocecal lymph nodes (ICLNs), where microbes are concentrated and an adequate immune response is triggered. Hence, ICLNs are at a crucial position in immune anatomy and control processes of the local immune system. Pathological alterations in ICLNs, such as reactive hyperplasia, lymphadenitis purulenta, or granulomatosa, can harbor a multitude of pathogens and commensals, posing a potential zoonotic risk in animal production. The aim of this study was to characterize the microbial diversity of unreactive ICLNs of slaughter pigs and to investigate community shifts in reactive ICLNs altered by enlargement, purulence, or granulomatous formations. Pyrosequencing of 16S rRNA gene amplicons from 32 ICLNs yielded 175,313 sequences, clustering into 650 operational taxonomic units (OTUs). OTUs were assigned to 239 genera and 11 phyla. Besides a highly diverse bacterial community in ICLNs, we observed significant shifts in pathologically altered ICLNs. The relative abundances of Cloacibacterium- and Novosphingobium-associated OTUs and the genus Faecalibacterium were significantly higher in unreactive ICLNs than in pathologically altered ICLNs. Enlarged ICLNs harbored significantly more Lactobacillus- and Clostridium-associated sequences. Relative abundances of Mycoplasma, Bacteroides, Veillonella, and Variovorax OTUs were significantly increased in granulomatous ICLNs, whereas abundances of Pseudomonas, Escherichia, and Acinetobacter OTUs were significantly increased in purulent ICLNs (P < 0.05). Correlation-based networks revealed interactions among OTUs in all ICLN groups, and discriminant analyses depicted discrimination in response to pathological alterations. This study is the first community-based survey in ICLNs of livestock animals and will provide a basis to broaden the knowledge of microbe-host interactions in pigs. PMID:24141125

  13. Toxoplasma gondii in Switzerland: a serosurvey based on meat juice analysis of slaughtered pigs, wild boar, sheep and cattle.

    PubMed

    Berger-Schoch, A E; Bernet, D; Doherr, M G; Gottstein, B; Frey, C F

    2011-11-01

    Toxoplasmosis is one of the most important zoonotic diseases worldwide and is caused by the protozoan Toxoplasma gondii. Besides vertical infection during pregnancy, humans can get infected post-natally either by peroral uptake of sporulated Toxoplasma oocysts or by ingestion of tissue cysts upon consumption of raw or undercooked meat. The aim of this study was to approximate the risk of human infection via meat consumption by estimating the seroprevalence of T. gondii in slaughtered animals in Switzerland and to compare data with prevalences assessed 10 years ago. The study included pigs, cattle, sheep and wild boar of different age groups and housing conditions whenever possible and applicable. A P-30-ELISA was used to detect T. gondii-specific antibodies and to determine seroprevalences in meat juice of slaughtered animals. A total of 270 domestic pigs (120 adults, 50 finishing, 100 free-ranging animals), 150 wild boars, 250 sheep (150 adults, 100 lambs) and 406 cattle (47 calves, 129 heifers, 100 bulls, 130 adult cows) were tested. Seropositivity increased with the age of the assessed animals. Independent of the age-group, the overall seroprevalence was lowest in wild boars (6.7%), followed by pigs (23.3%), cattle (45.6%) and sheep (61.6%), respectively. Conventional fattening pigs and free-ranging pigs surprisingly had comparable seroprevalences (14.0% and 13.0%, respectively). Unlike in other European countries, where generally a decrease in the number of seropositive animals had been observed, we found that the prevalence of seropositive animals, when compared with that of 10 years ago, had increased for most species/age groups. Conclusively, the results demonstrated a high seroprevalence of T. gondii in animals slaughtered for meat production and revealed that increasing age of the animals is a more important risk factor than housing conditions in Switzerland.

  14. Salmonella prevalence and characterization in a free-range pig processing plant: tracking in trucks, lairage, slaughter line and quartering.

    PubMed

    Hernández, Manuela; Gómez-Laguna, Jaime; Luque, Inmaculada; Herrera-León, Silvia; Maldonado, Alfonso; Reguillo, Lucía; Astorga, Rafael J

    2013-03-01

    New consumer tendencies are focused on products derived from systems which allow both a high animal welfare condition and a high food safety level. However, sometimes animal welfare regulations make the adoption of adequate bio-security measures difficult, representing a barrier for animal health and food safety. Thus the aim of this study was to determine the prevalence of Salmonella at different points of the pig slaughtering process (Trucks, Lairage, Slaughter line and Quartering, TLSQ) from pigs reared in free-range systems. From eight samplings a total of 126 Salmonella isolates out of 1160 different samples were recovered (10.86%). The highest percentage of isolates was detected at the points of pre-scalding (29/80, 36.25%), trucks (13/56, 23.21%), cecal contents (17/80, 21.25%), tonsils (14/80, 17.50%), ileocecal lymph nodes (13/80, 16.25%) and lairage (9/64, 14.06%). Furthermore, eighteen isolates were obtained from different environmental samples from slaughter line and quartering plant (knives and surface of tables) (5.63%) and three isolates at the quartering plant samples (ham, shoulder and loin) (3.75%). Fourteen different serotypes were isolated: Bredeney, Rissen, Derby, Typhimurium, Montevideo, Israel, Anatum, Emek, Monophasic Salmonella Typhimurium (mST), Choleraesuis, Durban, Kentucky, London and Sandiego. S. Typhimurium phage types U311, 193, 104b and UT were identified. Moreover, mST strain was phage typed as U311. From TLSQ1, TLSQ2 and TLSQ4, different strains of S. Derby, S. Rissen and S. Bredeney serotypes were isolated from pig and environmental samples, pointing to a potential cross contamination. Molecular typing (Pulsed-Field Gel Electrophoresis, PFGE) of these strains confirmed the cross contamination. In the remaining samplings, different serotypes were obtained in each sampled point of the chain, assuming that the isolated serotypes belonged to different epidemiological origins. Our results show the isolation of different serotypes of

  15. Simulation of the distribution of current density in the brain of slaughter pigs with the finite element method.

    PubMed

    Eike, H; Koch, R; Feldhusen, F; Seifert, H

    2005-04-01

    The current density in the brain of a slaughter pig during electric stunning was calculated and visualised with an finite element method computer model. The anatomic model of the pig's head was constructed with the computer programme Ansys. Ansys offers the possibility of calculating the current density between electrodes in any position using the mathematical "finite element method" model. After calculation the current density distribution can be visualised in planes in any direction through the pig's head. Our simulation confirmed the common practice of positioning the electrodes for electric stunning either eye to eye or eye to ear, because the highest current density through the brain was calculated for these positions. Setting the electrodes further caudally reduced the current density remarkably and, stunning is therefore not guaranteed. Additionally, this model showed for the first time that, due to their lower resistance, the nervus opticus and blood vessels conduct the current like wires into the brain.

  16. Reducing the length of time between slaughter and the secondary gonadotropin-releasing factor immunization improves growth performance and clears boar taint compounds in male finishing pigs.

    PubMed

    Lealiifano, A K; Pluske, J R; Nicholls, R R; Dunshea, F R; Campbell, R G; Hennessy, D P; Miller, D W; Hansen, C F; Mullan, B P

    2011-09-01

    The objective of this study was to evaluate whether altering the timing of the secondary anti-gonadotropin-releasing factor (GnRF) immunization closer to slaughter in male finishing pigs would reduce the increase in P2 fat depth (6.5 cm from the midline over the last rib), while still limiting the incidence of boar taint. Entire male pigs are immunized against GnRF to reduce the concentration of testicular steroids that in turn limits the incidence of boar taint. Additionally, testicle measurements and color measurements were taken to examine whether they could be used to differentiate nonimmunized entire males from immunized male pigs. A total of 175 Large White × Landrace entire male pigs aged 16 wk (59 kg of BW) were used in a completely randomized design with 5 treatment groups based on the time that pigs received the secondary immunization before slaughter. Pigs were housed in groups of 7 and randomly allocated to 1 of 5 treatments with 5 replicates per treatment. The treatment groups were as follows: no secondary immunization before slaughter, and the secondary immunization given at 2, 3, 4, or 6 wk before slaughter. The P2 fat depth levels were reduced (P = 0.054) with the secondary immunization closer to slaughter (11.7, 11.3, 12.8, 12.6, and 13.7 mm for no secondary immunization, secondary immunization at 2, 3, 4, and 6 wk before slaughter, respectively). Androstenone concentration did not exceed the generally accepted industry sensory threshold of 1.0 µg/g of fat, and both androstenone concentration in the adipose tissue and testosterone concentrations in the blood were suppressed (P < 0.001) in all immunized pigs regardless of timing of the secondary immunization compared with pigs that did not receive the secondary immunization. Skatole concentration of all pigs in the experiment did not exceed the generally accepted industry sensory threshold of 0.2 µg/g. Testes weight was reduced (P < 0.001) with increased time between slaughter and the secondary

  17. Detection of Pathogenic Yersinia Enterocolitica in Slaughtered Pigs by Cultural Methods and Real-Time Polymerase Chain Reaction

    PubMed Central

    Fois, Federica; Consolati, Simonetta Gianna; Salza, Sara; Tedde, Tiziana; Soro, Paolo; Collu, Carlo; Ladu, Daniela; Virgilio, Sebastiano; Piras, Francesca

    2015-01-01

    Healthy pigs carrying pathogenic to human Yersinia enterocolitica strains are the main source of entry into slaughterhouse, where cross-contamination of carcasses can happen. The aim of this work was to determine Y. enterocolitica prevalence in slaughtered pigs, investigating the presence of carriers in relation to carcass contamination. A total of 132 pig samples (tonsils, mesenteric lymph nodes, colon content, carcass surface) were collected from 4 Sardinian slaughterhouses. All the samples were examined by the ISO 10273:2003 method, and the prevalence was also determined by direct plating on CIN Agar. Moreover, to detect the ail positive Y. enterocolitica strains in enrichment broths and isolates a real-time polymerase chain reaction (PCR) was applied. Y. enterocolitica prevalence was 19% with direct plating and 12% with enrichment methods. Carcass surfaces and tonsils prevalence was 5.30% by direct plating, and 5.3% and 2.2%, respectively, by enrichment method. Tonsil samples showed an average contamination level of 3.2×103 CFU/g, while the mean value on carcass was 8.7×102 CFU/g. An overall prevalence of 9.8% of ail positive Y. enterocolitica broths was detected by RT-PCR, that found a higher prevalence in tonsils (7.5%) with respect to cultural methods, confirming the greater sensitivity of this technique when applied for tonsils and faeces samples. The results show a relatively low pathogenic Y. enterocolitica prevalence in pigs slaughtered in Sardinia. Good hygiene measures should be applied at slaughterhouse in order to prevent the entry of carriers and control carcass contamination. PMID:27800392

  18. The effect of weaner diet protein content and diet quality on the long-term performance of pigs to slaughter.

    PubMed

    Wellock, I J; Houdijk, J G M; Miller, A C; Gill, B P; Kyriazakis, I

    2009-04-01

    Short and long-term effects of manipulating dietary CP content and diet quality in weaner diets on health and performance of pigs were investigated in a 2 x 2 factorial combination of CP inclusion (high-CP, 230 g of CP/kg vs. low-CP, 170 g of CP/kg) and diet quality (high-quality, cooked cereals, and animal protein vs. low-quality, raw cereals, and plant protein). Diets were fed ad libitum for 14 d postweaning to pigs weaned at 29.4+/-3.1 d of age and 9.9+/-1.0 kg of BW. From d 14 to slaughter at 104+/-3 kg, all pigs were fed the same series of standard commercial diets. There were 15 replicates per treatment in the weaner phase (<30 kg) and 5 replicates per treatment in the grower-finisher phase (>30 kg). High-quality diets promoted gut health as indicated by improved fecal lactobacilli to coliform ratio (P=0.002) and decreased fecal enterotoxigenic Escherichia coli counts on d 11 postweaning (P=0.028), reducing the risk of postweaning diarrhea and improving pig health from weaning to the end of the weaner phase. Reducing CP content had no effect on gut health. High-CP (P=0.053) and high-quality (P=0.025) diets independently increased ADG during the first 14 d postweaning compared with low-CP and low-quality diets, respectively. There were no interactions between dietary CP content and quality on any of the response criteria investigated. Despite differences in the immediate postweaning period, there was no effect of manipulating diet quality or CP content for 2 wk postweaning on lifetime performance with pigs reaching slaughter weight in 128+/-7 d. These results indicate that high-quality diets may protect pig gut health during the immediate postweaning period. However, it may be possible to use less expensive, decreased quality weaner diets without any adverse effects on long-term performance when weaning older, heavier pigs and where health status, environmental control, and stock management are all maintained to a high standard.

  19. Use of infrared ocular thermography to assess physiological conditions of pigs prior to slaughter and predict pork quality variation.

    PubMed

    Weschenfelder, Angela V; Saucier, Linda; Maldague, Xavier; Rocha, Luiene M; Schaefer, Allan L; Faucitano, Luigi

    2013-11-01

    Infrared thermography (IRT) body temperature readings were taken in the ocular region of 258 pigs immediately before slaughter. Levels of lactate were measured in blood taken in the restrainer. Meat quality was assessed in the longissimus dorsi (LD), semimembranosus (SM), and adductor muscles. Ocular IRT (IROT) temperature was correlated with blood lactate levels (r=0.20; P=0.001), with pH taken 1hour postmortem (pH1: r=-0.18; P=0.03) and drip loss (r=0.20; P=0.02) in the LD muscle, and with pH1 in the SM muscle (r=-0.20; P=0.02). Potentially, IROT may be a useful tool to assess the physiological conditions of pigs at slaughter and predict the variation of important meat quality traits. However, the magnitude of the correlations is rather low, so a further development of image capture technique and further studies under more variable preslaughter conditions ensuring a larger pork quality variation are needed.

  20. Prevalence of Salmonella spp. and Yersinia enterocolitica in/on tonsils and mandibular lymph nodes of slaughtered pigs.

    PubMed

    Zdolec, Nevijo; Dobranić, Vesna; Filipović, Ivana

    2015-03-01

    A total of 156 tonsils and 156 mandibular lymph nodes from fattening pigs originating from 13 farms were sampled in Croatian slaughterhouses and examined for Salmonella spp. (n=78 per organ) and Yersinia enterocolitica (n=78 per organ) by cultural methods. Salmonella was isolated from two tonsils only, both originated from animals from the same farm (5.12%), while Y. enterocolitica were recovered from 26 tonsils (33.33%) which could be traced back to 10 farms. Salmonella was absent in mandibular lymph nodes, and Y. enterocolitica was isolated from eight lymph nodes (10.25%) which originated from six farms. Y. enterocolitica was present inside the lymph nodes of two pigs. The high prevalence of Y. enterocolitica in/on pig tonsils could be the result of cross-contamination during splitting the carcasses with head. This procedure may result in higher prevalence of Y. enterocolitica on surface of mandibular lymph nodes than in their depth. Traditional veterinary postmortem examination of pig halves will not necessarily contribute to cross-contamination with Salmonella or Yersinia under conditions of present slaughter practice.

  1. Assessment of factors influencing the within-batch seroprevalence of human enteropathogenic Yersinia spp. of pigs at slaughter age and the analogy with microbiology.

    PubMed

    Vanantwerpen, G; Berkvens, D; De Zutter, L; Houf, K

    2017-02-01

    The microbiologically and serologically-based prevalence of human enteropathogenic Yersinia spp. at moment of slaughter varies between pig farms due to different herd-level factors. A face-to-face questionnaire concerning a broad range of farm aspects (e.g., management and housing system, biosecurity, and hygiene measurements) was performed on one hundred farms. Factors influencing the seropositivity of 7047 pigs against human pathogenic Yersinia spp. were determined and compared to the microbiology. At the slaughterhouse, pieces of diafragm of on average 70 slaughter pigs per batch were sampled to determine the level of antibodies against enteropathogenic Yersinia spp. After univariable mixed-effect logistic regressions, variables that were related to the seropositivity (p<0.05) were included in a multivariable model (p<0.1). The factors remaining significantly associated in the latter model were an increasing number of piglet suppliers (zero up to eleven suppliers) (Odds Ratio=1.4), a high density of pig farms in the area (high versus low density) (Odds Ratio=2.3), the use of semislatted floors in the fattening pig unit (semi slatted floor versus fully slatted floor) (Odds Ratio=3.8) and the possibility of snout contact in the fattening pig unit (snout contact or not) (Odds Ratio=0.1). Decreasing the risk of infection with human enteropathogenic Yersinia spp. at moment of slaughter or during rearing is possible by changing farm management factors.

  2. Prevalence of Muscular Sarcosporidiosis in Slaughtered Domestic Pigs in Perak, Peninsular Malaysia

    PubMed Central

    Zainalabidin, Fazly Ann; Noorazmi, Muhamad Syamsul Naim; Bakri, Wan Normaziah Wan Omar; Sathaya, Geethamalar; Ismail, Mohd Iswadi

    2017-01-01

    Sarcosporidiosis is a disease caused by intracellular protozoan parasites, namely, Sarcocystis spp. In pigs, three species of Sarcocystis spp. have been recognised, including Sarcocystis meischeriana, Sarcocystis porcifelis and Sarcocystis suihominis. The aim of this study is to determine the prevalence of muscular sarcosporidiosis in pigs using the pepsin digestion technique. A total of 150 fresh heart, oesophagus and thigh muscle samples from 50 Yorkshire and Landrace pigs were collected from two local abattoirs in Perak from May to August 2014. All the fresh muscle samples were thoroughly examined for macrocyst-forming Sarcocystis spp. and processed using the peptic digestion technique to detect bradyzoites. The results from the muscle samples showed that 58% (29 out of 50) of the pigs were positive for Sarcocystis spp. These findings highlight the importance of implementing stringent measures for screening pigs in abattoirs for Sarcocystis spp. infection because this infection in pigs is a public health concern. PMID:28228924

  3. Herd-level risk factors for antimicrobial demanding gastrointestinal diseases in Danish herds with finisher pigs: A register-based study.

    PubMed

    Hybschmann, G K; Ersbøll, A K; Vigre, H; Baadsgaard, N P; Houe, H

    2011-02-01

    Endemic gastrointestinal (GI) diseases have a substantial negative impact on pig production, because, when present, they reduce animal welfare, productivity and generate high antimicrobial (AM) demand. In Danish legislation, AM can be prescribed only for therapeutic purposes. The objective of the study was to estimate the association between herd-level risk factors and the amount of AM use (AMU) in connection with GI diseases in finisher herds. We conducted a register-based cross-sectional study with repeated measurements from 2004 to 2007. Data were extracted from databases in the Danish Register of Veterinary Medicine, the Central Husbandry Register and the Danish Agriculture and Food Council. In total, 3192 pig herds with 26,973 records (quarters with prescriptions) were included. The outcome was presented as average AM use (measured as Animal Daily Dosage) for GI diseases per finishing pig per quarter per herd. Three potential herd-level risk factors were evaluated: herd size (number of finishers delivered for slaughter); herd health status (herds in the Specific Pathogen Free (SPF) System, conventional herds); and herd type (herds including only finishers, integrated herds). Data were analyzed using general linear mixed models with repeated measurements. Smaller herds had a larger AMU per finisher than larger herds. Integrated herds had lower AMU as compared with herds with only finishers. Herds within the SPF System had a larger decrease in AMU with increasing herd size compared to conventional herds. Significant regional differences in AMU were seen. Additionally, the results showed that other herd factors and veterinarians were more influential than the investigated herd risk factors. This illustrates the difficulties of characterising AM-demanding GI diseases in herds by the use of register data only.

  4. Comparison of risk-based versus random sampling in the monitoring of antimicrobial residues in Danish finishing pigs.

    PubMed

    Alban, Lis; Rugbjerg, Helene; Petersen, Jesper Valentin; Nielsen, Liza Rosenbaum

    2016-06-01

    In Denmark, a monitoring program for residues of antimicrobials in pork is in place involving annual testing of around 20,000 samples from finishing pigs corresponding to 0.1% of the animals slaughtered. Annually, zero to two samples are found above the maximum residue limit. Both authorities and industry have expressed interest in adjusting the monitoring to a risk-based system. The objective of this study was to assess the opportunities and consequences of the monitoring considering: 1) replacing the current bioassay with high-performance liquid chromatography-mass spectrometry (HPLC LC-MS/MS), 2) replacing kidney with muscles as sample matrix, and 3) using indicators to identify high-risk (HR) herds and increase sampling intensity in these herds, lowering sampling in the low-risk (LR) herds, while aiming at continued detection of similar numbers of test-positives at the lowest possible costs. A state-of-the-art stochastic scenario tree modelling approach including economic evaluation of different model outcomes was used. A total of six scenarios were run for penicillin and tetracycline, respectively. Relevant information was obtained through the literature, statistical analysis of existing data as well as consultations with laboratory and slaughterhouse experts. Abattoir recordings of chronic pleuritis were used as an indicator for finishing pig herds (HR=within-herd prevalence>40%). Such risk-based monitoring would have to use muscles and not kidneys, because of logistic challenges in identifying and storing of plucks until testing. However, the bioassay cannot be used on muscle tissue due to low sensitivity for tetracyclines. Different plausible combinations of sample sizes were also modelled. The HPLC LC-MS/MS method detected the same number of cases compared to the bioassay when kidney was used as matrix. HPLC LC-MS/MS has a higher sensitivity when used on muscle but it is almost twice as costly as the bioassay. Risk-based sampling resulted in detection of

  5. Influence of production system in local and conventional pig breeds on stress indicators at slaughter, muscle and meat traits and pork eating quality.

    PubMed

    Lebret, B; Ecolan, P; Bonhomme, N; Méteau, K; Prunier, A

    2015-08-01

    Sensory quality of pork is a complex phenotype determined by interactions between genetic and environmental factors. This study aimed at describing the respective influences of breed and production system on the development of pork quality. Plasma stress indicators and Longissimus muscle (LM) composition, physicochemical and sensory quality traits were determined in two contrasted breeds - the conventional Large White (LW, n=40) and the French local Basque (B, n=60). Pigs were reared in either a conventional (C; n=20 per breed), alternative (A; sawdust bedding and outdoor area, n=20 per breed) or extensive system (E; free-range, n=20 B). All the pigs from A and C systems were slaughtered at the same slaughterhouse, whereas B pigs from the E system were slaughtered at a local commercial abattoir. Major breed differences were found for almost all traits under study. LM from B pigs exhibited higher lipid, lower water and collagen concentrations, as well as lower collagen thermal solubility (P0.05) influence plasma stress indicators, LM chemical composition and physicochemical or sensory traits of pork. In contrast, within the B pigs, the E system affected the meat quality more. Lower plasma cortisol levels (P<0.05), but higher plasma lactate, creatine kinase and lactate dehydrogenase activities, and more skin lesions (P<0.05), indicating higher muscular activity during pre-slaughter handling, were found in pigs produced in the E compared with the C system. E pigs exhibited higher meat pH1 and pHu values and shear force (P<0.01) and exhibited lower lightness, hue angle and drip and thawing losses (P<0.01) compared with the C pigs, whereas LM lipid, protein or collagen concentrations were not affected. Regarding sensory traits, the E system produced redder meat, but did not impact the eating quality of pork. Altogether, this study demonstrates that differences in meat quality between B and LW breeds can be modulated by extensive pig production system.

  6. Microencapsulated sorbic acid and pure botanicals affect Salmonella Typhimurium shedding in pigs: a close-up look from weaning to slaughter in controlled and field conditions.

    PubMed

    Grilli, Ester; Foresti, Fabio; Tugnoli, Benedetta; Fustini, Mattia; Zanoni, Maria G; Pasquali, Paolo; Callaway, Todd R; Piva, Andrea; Alborali, Giovanni L

    2015-10-01

    The aim of this study was to assess the efficacy of a combination of sorbic acid, thymol, and carvacrol in reducing the prevalence and shedding level of Salmonella Typhimurium in pigs either in a controlled challenge environment or in a production setting. In the first study, 24 weaned piglets were separated in 4 isolation units (6 piglets/isolation unit). Each unit received either a basal diet (no treatment) or a microencapsulated mixture of sorbic acid, thymol, and carvacrol at 1, 2, or 5 g/kg of feed. After 21 d, pigs were orally challenged with 6 log10 colony-forming units of Salmonella Typhimurium. Blood samples and feces from rectal ampullae were collected every week. On d56 of the study, pigs were euthanized and necropsied to collect intestinal contents (jejunum through colon) and ileocecal lymph nodes. Samples were analyzed for Salmonella Typhimurium and serological analysis was also conducted. In the second study, an all-in-all-out multisite pig farm that was positive for monophasic Salmonella Typhimurium was followed throughout a production cycle from weaning to slaughter. Pigs received either a basal diet or the basal diet including 5 g/kg of the microencapsulated additive. Environmental, fecal, and blood samples were collected monthly, and cecal contents and ileocecal lymph nodes were collected at slaughter to isolate and enumerate Salmonella. The results indicate that the additive at 5 g/kg tended to reduce Salmonella fecal prevalence in both a controlled challenge (p=0.07) and in production conditions (p=0.03). Nevertheless, the additive did not reduce the number of pigs seropositive for Salmonella, nor it reduced the Salmonella prevalence at slaughter. The data indicate that these additives are not effective alone but must be used in conjunction with appropriate containment measures at lairage in order to prevent reinfection in pigs and to reduce the number of pigs carrying Salmonella entering the food chain.

  7. [Implementation and results of the EU-wide baseline studies on the prevalence of Salmonella spp. in slaughter and breeding pigs in Austria].

    PubMed

    Kostenzer, Klaus; Much, Peter; Kornschober, Christian; Lassnig, Heimo; Köfer, Josef

    2014-01-01

    The Member States of the European Union are following a common strategy on the control of Salmonella and other foodborne pathogens (Anonym, 2003). Within that framework baseline studies on the most relevant animal populations have been carried out. This paper describes the implementation and the results of the baseline studies on Salmonella spp. in slaughter and breeding pigs in Austria. A total of 647 slaughter pigs were sampled in 28 slaughterhouses between October 2006 and September 2007. Samples were taken from the ileocaecal lymphnodes to detect infection in pigs and from the surface of the carcasses to detect contamination. Out of the 617 datasets included in the final analysis, Salmonella prevalences of 2% in lymphnodes and 1.1% on the carcass surface were observed. S. Derby, S. Enteritidis and S. Typhimurium were the three most frequently identified serovars. In an additional study, a total of 252 holdings with breeding pigs has been sampled between January and December combined multiplier herds. Respectively prevalences of 5, 8, 5, 3 and 9.1% were obtained, with S. Typhimurium being the most frequently isolated serovar. Overall, compared to neighbouring Member States a rather low prevalence of Salmonella spp. in pigs was documented for Austria, in particular in slaughter pigs.The serovar distribution seemed to be similar throughout the pig populations, some also being represented in Austrian human isolates. Contamination of feed seems to play a minor role considering the overall low prevalence, but nevertheless has to be taken into account in any future control or monitoring strategy for Salmonella spp. in pigs.

  8. Effect of dietary inclusion of whole ear corn silage on stomach development and gastric mucosa integrity of heavy pigs at slaughter.

    PubMed

    Mason, Federico; Pascotto, Ernesto; Zanfi, Cristina; Spanghero, Mauro

    2013-12-01

    The effect of dietary inclusion of whole ear corn silage on stomach development and on the incidence of gastric lesions was studied in heavy pigs. Three groups of 14 castrated male pigs were fed a control cereal-based diet and two diets containing whole ear corn silage (15% or 30% DM) from 90 kg bodyweight to slaughter at 170 kg. The diets with whole ear corn silage increased the amount of neutral detergent fibre in the stomach contents, the weight of the organs and the area of the pyloric region. Follicular gastritis was significantly lower and gastritis less severe in pigs fed the whole ear corn silage diets than pigs fed the control diet. The inclusion of whole ear corn silage in the diet influenced the development of the stomach and reduced the incidence of gastritis in heavy pigs.

  9. Evaluation of rapid methods for diagnosis of tuberculosis in slaughtered free-range pigs.

    PubMed

    Cardoso-Toset, Fernando; Luque, Inmaculada; Amarilla, Shyrley Paola; Gómez-Gascón, Lidia; Fernández, Layla; Huerta, Belén; Carrasco, Librado; Ruiz, Pilar; Gómez-Laguna, Jaime

    2015-05-01

    Free-range pigs can be infected by Mycobacterium tuberculosis complex (MTC) and may contribute to the spread of bovine tuberculosis (bTB). In the present study, the diagnostic values of bacteriological culture, a duplex real-time quantitative PCR and an antibody ELISA were evaluated in an abattoir study of submandibular lymph nodes and serum samples from 73 pigs with and without lesions consistent with bTB. The duplex qPCR was an accurate method for diagnosis of TB in pigs (specificity 100%; sensitivity 80%). Combining qPCR with histopathology improved sensitivity and had very good concordance (κ = 0.94) with the reference method. Serological results suggest that the antibody ELISA can be used for monitoring herds but not individuals.

  10. Effects of feeding finisher pigs with chicory or lupine feed for one week or two weeks before slaughter with respect to levels of Bifidobacteria and Campylobacter.

    PubMed

    Jensen, A N; Hansen, L L; Baggesen, D L; Mølbak, L

    2013-01-01

    This study aimed to assess whether inclusion of chicory or lupine (prebiotics) in the diet of pre-slaughter pigs for just 1 or 2 weeks could change the composition of their intestinal microbiota, stimulate the growth of bifidobacteria and help to lower the amount of thermoplilic Campylobacter spp. (mainly Campylobacter jejuni and Campylobacter coli), which are a major cause of food-borne infections in humans. A total of 48 pigs that had an initial live weight of 90 kg were fed with either a lupine (organic concentrate with 25% blue lupine seeds), chicory (organic concentrate with 10% dried chicory roots) or control (100% organic concentrate) diet for 1 week (24 pigs) or 2 weeks (24 pigs) before slaughter. The Campylobacter spp. level in rectal faecal samples after 0, 1 and 2 weeks of feeding and in the luminal content from ileum, caecum and colon at slaughter was determined by direct plating on modified charcoal-cefoperazone-deoxycholate agar plates. DNA extracted from the luminal content of distal ileum and caecum was used for terminal restriction fragment length polymorphism (T-RFLP) analysis of the composition of intestinal microbiota and for measuring the amount of bifidobacterial and total bacterial DNA by quantitative real-time PCR (qPCR). Campylobacter spp. were excreted by all pigs and present in the luminal content from distal ileum to midway colon with particularly high numbers in the caecum, but the excretion was reduced by 10-fold in pigs fed lupines for 1 week as compared with control- and chicory-fed pigs (mean log(10) 2.9 v. 4.1 CFU/g; P < 0.05). The qPCR analysis showed that feeding with lupines resulted in higher levels of bifidobacteria in caecum as compared with the other diets (P < 0.05). T-RFLP analysis showed that four of the most abundant bacteria with terminal restriction fragment values >5% relative to the intensity of total abundance differed between the feed treatments (P < 0.05). Therefore, this study showed that even a short

  11. The increase of methicillin-resistant Staphylococcus aureus (MRSA) and the presence of an unusual sequence type ST49 in slaughter pigs in Switzerland

    PubMed Central

    2011-01-01

    Background In years past, methicillin-resistant S. aureus (MRSA) has been frequently detected in pigs in Europe, North America and Asia. Recent, yet sporadic studies have revealed a low occurrence of MRSA in Switzerland. In 2009, a monitoring survey of the prevalence and genetic diversity of methicillin-resistant S. aureus (MRSA) in slaughter pigs in Switzerland was conducted using methods recommended by the EU guidelines, and using a sampling strategy evenly distributed throughout the year and representative of the Swiss slaughter pig population. Monitoring should determine if the overall prevalence of MRSA in the entire country is increasing over the years and if specific multi-resistant MRSA clones are spreading over the country. Results In 2009, the nasal cavities of eight out of 405 randomly selected pigs were positive for MRSA, representing a prevalence of 2.0% (95% CI 0.9-3.9). The following year, 23 out of 392 pigs were positive for MRSA [5.9% prevalence (95% CI 3.8-8.7)]. Three multilocus sequence types (ST), four spa types and two types of staphylococcal cassette chromosome mec (SCCmec) elements were detected. The most frequent genotypes were ST398 (MLST)-(spa)t034-V(SCCmec) (n = 18) and ST49-t208-V (n = 7), followed by ST398-t011-V (n = 4), ST398-t1451-V (n = 1), and ST1-t2279-IVc (n = 1). The isolates displayed resistance to ß-lactams [mecA, (31/31); blaZ, (19/31)]; tetracycline [tet(M), (31/31); tet(K), (30/31)] (n = 31); macrolides and lincosamides [erm(C) (4/31) or erm(A) (18/31)] (n = 22); tiamulin [vga(A)v (9/31) or unknown mechanism (18/31)] (n = 27); trimethoprim [dfr(G) (18/31); spectinomycin [ant(9)-Ia (19/31) or unknown mechanism (3/31)] (n = 22); streptomycin [str (19/31)]; sulphamethoxazole (7/31) and ciprofloxacin (n = 1) (mechanisms not determined). Conclusions This study is the first to describe the presence of MRSA ST49 in slaughter pigs, and to demonstrate a significant and nearly three-fold increase of MRSA prevalence in pigs within

  12. Study on the Association between Tail Lesion Score, Cold Carcass Weight, and Viscera Condemnations in Slaughter Pigs

    PubMed Central

    Teixeira, Dayane Lemos; Harley, Sarah; Hanlon, Alison; O’Connell, Niamh Elizabeth; More, Simon John; Manzanilla, Edgar Garcia; Boyle, Laura Ann

    2016-01-01

    The aim of this study was to assess the relationship between tail lesions, cold carcass weight, and viscera condemnations in an Irish abattoir. The following data were collected at the evisceration point from every third pig slaughtered over 7 days: farm identification, sex, tail lesion score, viscera inspection outcome, and cold carcass weight. Tail lesions were scored according to a 5-point scale. Disease lesions responsible for lung (pleurisy, pneumonia, and abscess), heart (pericarditis), and liver (ascariasis) condemnation were recorded based on the decision of the veterinary inspector (VI). Data on 3,143 pigs from 61 batches were available. The relationship between disease lesions, tail lesion score, and cold carcass weight was studied at individual carcass level, while the relationship between disease lesions and tail lesion score was studied at both carcass and batch level. Tail lesions (score ≥1) were found in 72% of the study population, with 2.3% affected by severe tail lesions (scores ≥3). Pleurisy (13.7%) followed by pneumonia (10.4%) showed the highest prevalence, whereas the prevalence of ascariasis showed the greatest variation between batches (0–75%). Tail lesion score, pleurisy, pleuropneumonia, and pericarditis were associated with reductions in carcass cold weight (P ≤ 0.05) ranging from 3 to 6.6 kg. Tail lesion score was associated with condemnations for pleurisy, pneumonia, and pleuropneumonia (P ≤ 0.05) at a batch level. VI shift was associated with condemnations for pneumonia, pleuropneumonia, and pericarditis (P ≤ 0.05) at a carcass level and with pneumonia at a batch level. Sex was not associated with viscera condemnations but males were more likely to be affected by tail lesions. The relationship between overall tail lesion score and the lung diseases at batch level supports the relationship between poor health and poor welfare of pigs on farms. The inclusion of tail lesion scores at post-mortem meat inspection

  13. Experimental infection of slaughter pigs with classical swine fever virus: transmission of the virus, course of the disease and antibody response.

    PubMed

    Laevens, H; Koenen, F; Deluyker, H; de Kruif, A

    1999-08-28

    The spread of classical swine fever virus was investigated in an isolation unit containing four pens, each containing six slaughter pigs. One pig in the middle pen of three adjacent pens was inoculated intramuscularly and intranasally with the virus. The fourth pen was located in a separate compartment. The pens were visited in a strict order to study, first, the effect of indirect contact via contaminated clothing and footwear on the spread of the virus to adjacent pens and, secondly, the airborne transmission of the virus between compartments. The pigs were examined and blood samples were taken every other day for 62 days for virological and serological analyses. The virus was highly contagious for the five pigs that were in direct contact with the inoculated pig, but spread to the other pens only after all the pigs in the originally infected pen had become viraemic. The spread of the virus was promoted by contaminated clothing and footwear, but airborne transmission contributed considerably to the spread of the virus within the pighouse. The first clinical signs observed after the virus was introduced into a pen were decreased feed intake, increased mean rectal temperature and apathy. Neither the clinical course of the infection, nor the pattern of seroconversion observed over time, was affected by the differences in the intensity of contact with the virus between the pigs in the different pens.

  14. Descriptive analysis of the prevalence and the molecular epidemiology of Mycobacterium avium complex-infected pigs that were slaughtered on the main island of Okinawa.

    PubMed

    Hibiya, K; Kazumi, Y; Nishiuchi, Y; Sugawara, I; Miyagi, K; Oda, Y; Oda, E; Fujita, J

    2010-09-01

    Recent genetic studies have revealed that several epidemiological factors affect Mycobacterium avium complex (MAC) infection in pig populations. However, mechanisms underlying the spread of MAC infection among hog farms have not been clarified. In consideration of this situation, we cross-sectionally investigated the mechanisms underlying the spread of MAC on the island of Okinawa. Pigs slaughtered (n=706,763) and 331 hog farms on Okinawa were surveyed during the years 2002-2004. Two outbreaks of MAC infection were occurred in several farms during survey period. Bacteria were isolated from randomly selected pigs and genotype of isolates was determined by using genetic finger printing methods with the insertion sequence (IS) 1245 restriction fragment length polymorphism (RFLP). Most isolates had large numbers of IS1245 copies, while strains with low copy numbers of IS1245 and isolates without IS1245 were seen in few farms. MACs strains were repeatedly isolated from pigs of the affected farms during the survey period. Those farms with an identical pig rearing systems showed synchronic changes in the prevalence of MAC infection. An industrial farm without an outbreak had an independent pig flow, but maintained distinct MAC strains. Multivariate analysis did not reveal independent factors for the prevalence of the MAC infection. These findings suggest that there were three clusters distinguished genetically in the main island of Okinawa, which were potentially spread by common pig flow. However, the outbreaks occurred because of unspecified conditions on each farm environment.

  15. Farm-specific lineages of methicillin-resistant Staphylococcus aureus clonal complex 398 in Danish pig farms.

    PubMed

    Espinosa-Gongora, C; Larsen, J; Moodley, A; Nielsen, J P; Skov, R L; Andreasen, M; Guardabassi, L

    2012-10-01

    The objective of this study was to investigate the genetic diversity of methicillin-resistant Staphylococcus aureus (MRSA) clonal complex (CC) 398 using pulsed-field gel electrophoresis (PFGE). Dust and pigs at five age groups were sampled in six Danish MRSA-positive pig farms. MRSA CC398 was isolated from 284 of the 391 samples tested, including 230 (74%) animal and 54 (68%) environmental samples. PFGE analysis of a subset of 48 isolates, including the six strains previously isolated from farm workers, revealed the existence of farm-specific pulsotypes. With a single exception, human, environmental and porcine isolates originating from the same farm clustered together in the PFGE cluster analysis, indicating that spread of MRSA CC398 in Danish pig farms is mainly due to clonal dissemination of farm-specific lineages that can be discriminated by PFGE. This finding has important implications for planning future epidemiological studies investigating the spread of CC398 in pig farming.

  16. Rapid testing and quantification of Salmonella in ileocaecal lymph nodes of Austrian pigs slaughtered for consumption.

    PubMed

    Mann, Evelyne; Wagner, Martin; Schmoll, Friedrich; Slaghuis, Jörg; Schönenbrücher, Holger; Mester, Patrick

    2014-10-01

    Traditionally, quantitative microbial risk assessment (QMRA) is based on culture-dependent technologies. However, molecular quantification could forge additional, detailed information. A prerequisite of quantitative real-time PCR in animal science is a tissue preparation method where large volumes of tissue material can be reduced and particularly target cells can be concentrated. An easy-to-use sample preparation method for food (Matrix-Lysis) was recently adapted to tissues and now permits quantification of target cells from up to 5 g of organic matrix. The aim of this study was to examine the suitability of Matrix-Lysis for quantification of Salmonella in porcine ileocaecal lymph nodes (ICLNs). After demonstrating constant recovery rates, ICLNs from 540 pigs were examined for Salmonella spp. with Matrix-Lysis. Samples were also analysed using ISO 6579:2002, a combined enrichment/qPCR method and a lateral flow test. It could be shown that qPCR coupled with Matrix-Lysis can contribute to QMRA in food safety by enabling reproducible quantitative data, even at low contamination rates.

  17. Surveillance and antimicrobial resistance of Salmonella strains isolated from slaughtered pigs in Spain.

    PubMed

    Astorga, Rafael Jesús Márquez; Salaberria, Aurora Echeita; García, Alfonso Maldonado; Jimenez, Silvia Valdezate; Martinez, Alfonso Carbonero; García, Ana Aladueña; Casas, Antonio Arenas

    2007-06-01

    The prevalence of and the antibiotic resistance shown by Salmonella isolated from pigs in Andalusia (southern Spain) is reported. Salmonella enterica was recovered from 40 (33%) of 121 sampled herds, and a total of 65 isolates were serotyped. The most common Salmonella serotypes were Typhimurium and Rissen (30.7% each); others included Derby (9.2%), Brandenburg (9.2%), Newport (7.7%), Bredeney (4.6%), Anatum (3.0%), Hadar (1.5%), and Goldcoast (1.5%). One strain (1.5%) belonging to the monophasic variant of the Typhimurium serotype (Salmonella 4,5,12:i: -) was also detected. Definitive phage type (DT) 104b was the most common Typhimurium phage type isolated. These Salmonella strains were resistant to various antimicrobial agents, including tetracycline (84.6%), streptomycin (69.2%), neomycin (63.0%), sulfonamides (61.5%), ampicillin (53.8%), and amoxicillin (53.8%). All isolates were fully susceptible to ceftriaxone, ciprofloxacin, and colistin. Thirty-nine strains (64%) resistant to four or more antimicrobial agents were defined as multidrug resistant. Multidrug resistance profiles were observed in Salmonella serotypes Typhimurium, Rissen, Brandenburg, Bredeney, a monophasic variant, Gold-coast, Hadar, and Anatum, with serotypes Typhimurium and Brandenburg showing the most complicated resistance patterns (resistant to > or = 11 drugs).

  18. Evaluation of the nasal microbiota in slaughter-age pigs and the impact on nasal methicillin-resistant Staphylococcus aureus (MRSA) carriage

    PubMed Central

    2014-01-01

    Background The nasal microbiota of pigs has been poorly assessed but could play a role in carriage of important microorganisms such as methicillin-resistant Staphylococcus aureus (MRSA). The objectives of this study were to describe the nasal microbiota in slaughter age pigs, to evaluate the impact of farm management on the nasal microbiota and to provide a preliminary assessment of the influence of the microbiota on MRSA carriage. Results Nasal swabs were collected from five MRSA positive and eight MRSA negative pigs on one farm that used a liquid feeding system and routine tylosin treatment, and seven MRSA negative pigs from an antibiotic-free farm that used conventional feeding. A total of 946310 sequences passed all quality control filters. The number of sequences per sample ranged from 4307 to 165656 (mean 56092, SD 40007). CatchAll analysis of richness predicted a mean of 1749 OTUs (range 213–3736, SD 996). Overall, 6291 OTUs were identified, yet 5125 (81%) were identified less than 10 times and the 12 most abundant OTUs accounted for 80.7% of sequences. Proteobacteria predominated in all but two samples. Liquid-fed/tylosin-exposed pigs had significantly lower relative abundances of Verrucomicrobia (P = 0.004), Fibrobacteres (P = <0.0001) and sequences unclassified at the phylum level (P = 0.028). When comparing only liquid-fed pigs, MRSA carriers had significantly more Bacteroidetes (P = 0.037) than MRSA negative pigs. 124 genera were identified, with Moraxella accounting for 35.4% of sequences. In the Jaccard index tree, five of eight MRSA positive pigs clustered closely together, as did six of the seven conventionally-fed pigs. A significant difference was identified between conventional and liquid-fed pigs using parsimony test with the Jaccard (P < 0.001) but not the Yue&Clayton (P = 0.26) index. There were no significant differences between MRSA positive and negative pigs (P = 0.133 and 0.175). OTUs belonging to Firmicutes

  19. Early pathogenesis of classical swine fever virus (CSFV) strains in Danish pigs.

    PubMed

    Lohse, Louise; Nielsen, Jens; Uttenthal, Ase

    2012-10-12

    Host-virus interactions play an important role for the clinical outcome of classical swine fever virus (CSFV) infections in pigs. Strain virulence, host characteristics and environment are all factors that markedly influence disease severity. We tested CSFV strains of varying virulence in an experimental set-up, reducing the influence of host and environmental factors. Thus, weaner pigs were inoculated with one of 4 CSFV strains in order to compare the pathogenesis for a 3-week-period after infection. CSFV strains selected were 2 new and 2 previously characterized. None of these strains had been tested in Danish outbred pigs before. Clinical observations grouped the infected pigs into two different categories reflecting either non-specific, mainly gastro-intestinal, problems, or severe disease including high fever within the first week after inoculation. Gross-pathological findings varied between strains, however, lymphoid atrophy and growth retardation represented a consistent finding for all 4 strains. Virus distribution, viral load and in particular virus persistence differed, but supported present practice that recommends lymphoid tissue, most optimal tonsil and lymph nodes, as target material to be applied for early laboratory diagnosis. The present study demonstrated constraints associated with early detection of infections with CSFV strains of low virulence. Since neither clinical symptoms nor pathological lesions observed with these strains constituted characteristic signs of CSF, the risk of neglecting a CSF suspicion is immediate. Therefore, topical information on new outbreaks and continuous enhancement of an efficient surveillance system is of great importance to prevent further spread of CSF within the pig population.

  20. Detection, enumeration and characterization of Yersinia enterocolitica 4/O:3 in pig tonsils at slaughter in Northern Italy.

    PubMed

    Bonardi, Silvia; Alpigiani, Irene; Pongolini, Stefano; Morganti, Marina; Tagliabue, Silvia; Bacci, Cristina; Brindani, Franco

    2014-05-02

    Tonsils from 150 pigs slaughtered at 270 days or older were tested for Yersinia enterocolitica with different cultural methods. Samples were collected in three different abattoirs of Northern Italy between April and November 2012 and were analysed by direct plating on cefsulodin-irgasan-novobiocin (CIN) agar and by enrichment procedures following the ISO 10273:2003 reference method. Twenty-three (15.3%) samples were positive: 22 tonsils (14.7%) were positive for human pathogenic Y. enterocolitica bio-serotype 4/O:3 and one tonsil (0.7%) for Y. enterocolitica bio-serotype 1A/7,8-8,8,19. Seventeen samples out of 23 (73.9%) were positive by direct plating method. Among the enrichment procedures, the best recovery rate (8 positives out of 23; 34.8%) was obtained by the two-day enrichment in peptone-sorbitol-bile (PSB) broth followed by plating on CIN agar plates. The two-day enrichment in PSB followed by potassium hydroxide (KOH) treatment before plating onto CIN agar gave 7 positives out of 23 (30.4%), decreasing to 3 positives (13.0%) without KOH treatment. The worst results were obtained by prolonged (five days) enrichment in PSB, with or without KOH treatment, followed by plating on CIN agar: 4.3% (1 out of 23) and 0.0% recovery rates, respectively. The mean concentration was 1.9 × 10(4)CFU/g, with a minimum of 1.0 × 10(2)CFU/g and a maximum of 5.8 × 10(4)CFU/g, thus demonstrating that tonsils may play an important role in contamination of pluck sets, carcasses, and slaughterhouse environment. Prevalence of virulence genes among the Y. enterocolitica 4/O:3 isolates was as follows: 12/22 (54.5%) for yadA, 21/22 (95.5%) for ail, 21/22 (95.5%) for inv and 22/22 (100%) for ystA. All Y. enterocolitica 4/O:3 isolates were sensitive to amoxicillin/clavulanic acid, ciprofloxacin and ceftazidime and resistant to ampicillin and cephalotin. High proportions of 4/O:3 isolates (95%) were sensitive to cefotaxime, gentamicin, kanamicin and nalidixic acid. High levels of

  1. Bayesian analysis of response to selection: a case study using litter size in Danish Yorkshire pigs.

    PubMed Central

    Sorensen, D; Vernersen, A; Andersen, S

    2000-01-01

    Implementation of a Bayesian analysis of a selection experiment is illustrated using litter size [total number of piglets born (TNB)] in Danish Yorkshire pigs. Other traits studied include average litter weight at birth (WTAB) and proportion of piglets born dead (PRBD). Response to selection for TNB was analyzed with a number of models, which differed in their level of hierarchy, in their prior distributions, and in the parametric form of the likelihoods. A model assessment study favored a particular form of an additive genetic model. With this model, the Monte Carlo estimate of the 95% probability interval of response to selection was (0.23; 0.60), with a posterior mean of 0.43 piglets. WTAB showed a correlated response of -7.2 g, with a 95% probability interval equal to (-33.1; 18.9). The posterior mean of the genetic correlation between TNB and WTAB was -0.23 with a 95% probability interval equal to (-0.46; -0.01). PRBD was studied informally; it increases with larger litters, when litter size is >7 piglets born. A number of methodological issues related to the Bayesian model assessment study are discussed, as well as the genetic consequences of inferring response to selection using additive genetic models. PMID:10978292

  2. Break-even analysis of costs for controlling Toxoplasma gondii infections in slaughter pigs via a serological surveillance program in the Netherlands.

    PubMed

    van Asseldonk, M; van Wagenberg, C P A; Wisselink, H J

    2017-03-01

    Toxoplasma gondii (T. gondii) is a food safety hazard which causes a substantial human disease burden and cost-of-illness. Infected pig meat is a common source of toxoplasmosis. A break-even analysis was conducted to estimate the point for which the intervention cost at fattening pig farms equaled the cost of averted human disease burden and cost-of-illness minus the costs of a T. gondii surveillance program. The surveillance program comprised serological testing of blood samples taken at slaughter. Break-even points were determined given alternative levels of the effectiveness of the intervention program (10% up to 90% in steps of 10%), the value of an averted DALY (20,000, 50,000 and 80,000 Euro), and threshold of sample prevalence for a farm to be under intervention (5% up to 50% out of 20 samples in steps of 5%). Since test characteristics are a determining factor in the break-even analysis, and literature is inconclusive concerning sensitivity (se) and specificity (sp) of the serological test kit used, two alternative sets of assumptions were analysed. The estimated maximum costs of an intervention if only benefits for domestic consumers were accounted amounted approximately 2981 Euro (se=98.9% and sp=92.7%) versus 4389 Euro (se=65.2% and sp=97.4%) per year per fattening pig farm under intervention assuming an effectiveness of 50%, 50,000 Euro per averted DALY and threshold T. gondii sample prevalence of 5% for a farm to be under intervention. Since almost 80% of the gross domestic production is exported corresponding break-even values increased up to 12,034 Euro and 18,366 Euro if benefits for consumers abroad were included as well. Empirical research to strengthen the knowledge about the efficacy of a farm intervention measures is recommended.

  3. The effects of lairage time and handling procedure prior to slaughter on stress and meat quality parameters in pigs.

    PubMed

    Dokmanović, M; Velarde, A; Tomović, V; Glamočlija, N; Marković, R; Janjić, J; Baltić, M Ž

    2014-10-01

    Lairage time (short - 8min to 2.7h, n=28 vs. long - 14 to 21.5h, n=72) and pig handling (gentle - no use of stick or electric prod, pig not slipping, falling, nor emitting high-pitched vocalizations vs. rough - where any of these occurred) effects on pig stress and meat quality were measured. Blood lactate and cortisol, plus post-mortem pH (pH60min; pH24h), temperature (T60min), drip loss, sensory and instrumental color and meat quality for the longissimus dorsi, pars lumbalis derived meat were determined. Carcass rigor mortis and skin damages were measured. Lairage time significantly affected blood lactate, carcass rigor mortis, skin damages, drip loss, color and meat quality. Handling procedure influenced blood lactate, pH60min and T60min. Long lairage was more stressful, and was detrimental to carcass quality, but caused better meat quality compared to short lairage. Rough handling was related to higher lactate and lower meat quality.

  4. Serotypes, antimicrobial susceptibility, and minimal inhibitory concentrations of Actionbacillus pleuropneumoniae isolated from slaughter pigs in Taiwan (2002-2007).

    PubMed

    Yang, Cheng-Yao; Lin, Chao-Nan; Lin, Chuen-Fu; Chang, Tsung-Chou; Chiou, Ming-Tang

    2011-02-01

    In total, 211 isolates of A. pleuropneumoniae were collected from pigs with hemorrhagic pneumonia at slaughterhouses during 2002-2007. Serotypes, antimicrobial susceptibility and minimum inhibitory concentration (MIC) values were determined for each isolate of A. pleuropneumoniae to 10 antimicrobial agents. Serovar 1 of A. pleuropneumoniae was predominant in Taiwan in 138 of the 211 isolates, followed by serovars 2 and 5. More than 90% of collected isolates were sensitive to ceftiofur, cephalothin, and chloramphenical. However, lincospectin and gentamicin were relatively less susceptible with sensitivities of only 2.4 and 5.7%, respectively. Additionally, ceftiofur had the highest in vitro activity with an MIC(50) of 2.2 µg/ml, followed by cephalothin (2.7 µg/ml) and chloramphenicol (7.9 µg/ml). Lincospectin had the least activity with MIC(50) and MIC(90) values of 73.9 and 114.5 µg/ml, respectively. The data indicate that ceftiofur and cephalothin were extremely active against A. pleuropneumoniae and with minimum MIC values. These drugs are suitable for controlling and treating hemorrhagic pleuropneumonia outbreaks in swine.

  5. Biocide and antibiotic susceptibility of Salmonella isolates obtained before and after cleaning at six Danish pig slaughterhouses.

    PubMed

    Gantzhorn, Mette Rørbæk; Pedersen, Karl; Olsen, John Elmerdahl; Thomsen, Line Elnif

    2014-07-02

    Salmonella sp. continues to be one of the most important foodborne pathogens. Control measures in terms of cleaning and disinfection on food production plants are very important for limiting the risk of contaminated food products to reach the consumer. In the last decade concern has arisen that bacteria exposed to disinfectants can develop resistance toward disinfectants and can have a higher risk of developing antibiotic resistance. The objectives of this study were to examine the prevalence of biocide resistant Salmonella sp. in Danish pig slaughterhouses, to evaluate if there was a correlation between susceptibilities to biocides and antibiotics, and to examine if cleaning and disinfection select isolates with changed susceptibility toward biocides or antibiotics. Salmonella sp. was isolated from the environment in Danish pig slaughterhouses before and after cleaning and disinfection. The susceptibility toward three different biocides, triclosan and two commercial disinfection products: Desinfect Maxi, a quaternary ammonium compound, and Incimaxx DES, an acetic compound, was determined. We found no resistance toward the biocides tested, but we did find that isolates obtained after cleaning had higher minimum inhibitory concentration (MIC) values toward one of the disinfectants (Incimaxx DES) compared to isolates obtained before cleaning and disinfection. This could indicate selection of strains that are more tolerant, due to the cleaning and disinfection. Furthermore, we found that there was a weak statistical correlation between MICs toward the biocides and some antibiotics, but no difference in log(MIC)s toward antibiotics between isolates obtained before and after cleaning, nor did we find any difference in the number of resistances of isolates obtained before and after cleaning and disinfection.

  6. An appraisal of the use of meat-juice serology monitoring data for estimating prevalence of cecal Salmonella carriage of pigs at slaughter by means of herd-level and animal-level simulation.

    PubMed

    Barron, Ursula Gonzales; Soumpasis, Ilias; Butler, Francis; Duffy, Geraldine

    2009-02-01

    Some attempts have been made to elucidate the association between positive serology and Salmonella detection by bacterial culture in individual pigs and pig herds. This study aimed to appraise whether the existing knowledge on such association provides grounds for the utilization of serology monitoring data for predicting Salmonella subclinical infection of pigs entering the abattoir. Serology test results of pig carcasses (taken at abattoirs) originating from 436 representative active herds in Ireland were utilized to estimate the overall cecal Salmonella carriage of Irish slaughter pigs. To this effect, two separate simulations were conducted using (i) herd-level regression data and (ii) animal-level sensitivity (0.2890) and specificity (0.8895) data, which were extracted from published articles. The herd-level approach estimated a moderate prevalence of cecal Salmonella carriage of 0.222 (sigma = 0.094; 95% confidence interval [CI]: 0.069 to 0.431), which matched closely the mean prevalence value from the surveys' validation data of Salmonella-positive cecal samples (n = 1,098) obtained at Irish abattoirs (0.215; 95% CI: 0.192 to 0.240). The animal-level simulation generated an output distribution with slightly more uncertainty (sigma = 0.102 and 95% CI: 0.146 to 0.537) and a higher estimate of cecal carriage (0.312), which was an effect of the low relative sensitivity of serology, common under field conditions. While the herd-level simulation appeared to be technically more appropriate, since its correlation is only moderate, further elucidation of other factors related to subclinical infection should be attained for their incorporation in prospective dynamic on-farm models, which would be useful in the ultimate goal of estimating the risk of carcass contamination during slaughter.

  7. Religious slaughter in Italy.

    PubMed

    Cenci-Goga, B T; Mattiacci, C; De Angelis, G; Marini, P; Cuccurese, A; Rossi, R; Catanese, B

    2010-06-01

    This research aims to understand the prevalence of religious slaughter practices in Italy. Two different ways of slaughtering animals are identified. Conventional slaughter is performed with prior stunning; kosher slaughter is practiced without stunning. Halal slaughter is performed for most animals without stunning. Halal slaughter with prior stunning is acceptable for 5.90% of small ruminants. For Halal slaughter in Italy, the terms "religious slaughter with stunning" and "religious slaughter without stunning" should be used to differentiate religious slaughter practices, keeping animal welfare in perspective.

  8. Impact of the amount of straw provided to pigs kept in intensive production conditions on the occurrence and severity of gastric ulceration at slaughter.

    PubMed

    Herskin, Mette S; Jensen, Henrik E; Jespersen, Anna; Forkman, Björn; Jensen, Margit B; Canibe, Nuria; Pedersen, Lene J

    2016-02-01

    This study examined effects of the amount of straw offered on occurrence and severity of gastric lesions in pigs kept in pens (18 pigs, 0.7 m(2)/pig) with partly slatted flooring and 10, 500 or 1000 g straw/pig/day from 30 kg live weight. The pigs had ad libitum access to dry feed. Forty-five pigs were used, three from each of 15 pens. After euthanization, the dimension of the non-glandular region of the stomach was measured. Lesions were characterized and scored. Irrespective of straw provided, 67% of the pigs showed signs of gastric pathology. Pigs provided with 500 or 1000 g straw were pooled as 'permanent access'. The proportion of pigs with ulcerations was reduced by permanent access to straw (7 vs. 33%; P<0.05), suggesting that permanent access to straw may improve animal health, and be considered as one possible strategy to limit gastric ulceration in pigs.

  9. Antimicrobial reduction measures applied in Danish pig herds following the introduction of the "Yellow Card" antimicrobial scheme.

    PubMed

    Dupont, Nana; Diness, Line Hummelmose; Fertner, Mette; Kristensen, Charlotte Sonne; Stege, Helle

    2017-03-01

    Following introduction of the antimicrobial restrictive "Yellow Card Scheme" in summer 2010, a rapid decrease in the Danish national pig antimicrobial consumption was observed. The aims of this study were to (i) investigate which measures had been implemented to reduce the antimicrobial consumption according to farmers and veterinarians and (ii) where possible, investigate if said measures were reflected in the herds' antimicrobial purchase data. Based on national register data from VetStat and the Central Husbandry Register, the study population was selected among Danish pig herds which had decreased their annual antimicrobial consumption with ≥10% following the introduction of the Yellow Card Scheme comparing June 1, 2009-May 31, 2010 to June 1, 2010-May 31, 2011. Subsequently, questionnaire surveys of both farmers and veterinarians were carried out, resulting in responses from 179 farmers accounting for 202 herds (response ratio: 83%) and 58 veterinarians accounting for 140 herds. Prior to the introduction of the Yellow Card Scheme, 24% of the participating herds had an antimicrobial consumption for one or more age groups which exceeded the Yellow Card Scheme threshold values on antimicrobial consumption, while 50% of the herds had an antimicrobial consumption below the national average. The measures most frequently stated as having contributed to the antimicrobial reduction were increased use of vaccines (52% of farmers; 35% of the veterinarians), less use of group medication (44% of the farmers; 58% of the veterinarians) and staff education (22% of the farmers; 26% of the veterinarians). Reduced usage of antimicrobials for oral use accounted for 89% of the total reduction in antimicrobial use. Among the farmers, 13% also stated that change in choice of product had contributed to reducing their antimicrobial consumption. However, when analyzing purchase data, no general trend was seen towards a larger purchase of products with a higher registered dosage per

  10. Cryptosporidium and Giardia in different age groups of Danish cattle and pigs--occurrence and management associated risk factors.

    PubMed

    Maddox-Hyttel, Charlotte; Langkjaer, Rikke B; Enemark, Heidi L; Vigre, Håkan

    2006-10-10

    To obtain information both about the prevalence of Giardia and Cryptosporidium in Danish cattle and pigs as well as the possible influence of different management systems on the occurrence and intensity of infection, we conducted an epidemiological survey comprising 50 randomly selected dairy and sow herds, respectively. Each herd was visited once for the collection of faecal samples and registration of basic management parameters. Faecal samples were collected from three different age groups of animals, i.e. 5 sows/cows, 10 nursing piglets/calves less than 1 month, and 10 weaner pigs 8-45 kg/calves 1-12 months. The faecal samples were purified and the number of (oo)cysts quantified. The study revealed an age-specific herd prevalence of Cryptosporidium of 16, 31 and 100% for sows, piglets and weaners, respectively, and of 14, 96 and 84% for cows, young calves and older calves, respectively. For Giardia the age-specific herd prevalence was 18, 22 and 84% for the sows, piglets and weaners, while for cattle herds the prevalence was 60, 82 and 100% for cows, young calves and older calves, correspondingly. The (oo)cyst excretion levels varied considerably both within and between herds for all age groups. Risk factors were evaluated by using proportional odds models with (oo)cyst excretion levels divided into four categories as response. Among the numerous risk factors examined, only a few were demonstrated to have a statistically significant influence, e.g. the use of an empty period in the calf pen between introduction of calves for both parasites had a protective effect in young calves. For weaners, use of straw in the pen and high pressure cleaning between batches of weaners had a preventive effect against higher Cryptosporidium oocyst excretion levels.

  11. Feeding level in the period previous to the late fattening phase influences fat composition at slaughter in free-ranged Iberian pigs.

    PubMed

    Daza, Argimiro; Mateos, Arancha; Rey, Ana Isabel; Lopez-Bote, Clemente Jose

    2005-08-01

    A group of 11 pigs was fed with 70 g feed per kg of metabolic weight (H pigs) and another group of 11 pigs was fed with 50g feed per kg of metabolic weight (L pigs). In both experimental groups (H and L pigs), it was observed that the higher initial proportion of C16:0, C18:0 and C18:2 (n-6) in backfat at the beginning of the free-range feeding period, the greater decrease rate of these fatty acid proportions regarding weight gain during the free-range fattening period took place. On the other hand, the greater initial proportion of C18:1 (n-9), the smaller increase rate in the concentration of this fatty acid was observed. The intramuscular neutral lipids from L pigs had higher C18:3 (n-3) and lower proportions of monounsaturated fatty acids (MUFA) than those from H pigs, while intramuscular polar lipids from L pigs had significantly higher proportions of C18:0, PUFA, C18:3 (n-3) and (n-3) and significantly lower MUFA and C18:1 (n-9) proportions than those from H pigs. The alpha-tocopherol concentration found in Longissimus dorsi from L pigs was significantly higher (p < 0.012) than those from H pigs.

  12. Time to Loss of Consciousness and Its Relation to Behavior in Slaughter Pigs during Stunning with 80 or 95% Carbon Dioxide

    PubMed Central

    Verhoeven, Merel; Gerritzen, Marien; Velarde, Antonio; Hellebrekers, Ludo; Kemp, Bas

    2016-01-01

    Exposure to CO2 at high concentration is a much debated stunning method in pigs. Pigs respond aversively to high concentrations of CO2, and there is uncertainty about what behaviors occur before and after loss of consciousness. The aim was to assess timing of unconsciousness in pigs during exposure to high concentrations of CO2 based on changes in electroencephalogram (EEG) activity and the relation with the behaviors sniffing, retreat and escape attempts, lateral head movements, jumping, muscular contractions, loss of posture, and gasping. Pigs (108 ± 9 kg) were randomly assigned to 80% CO2 (80C, n = 24) or 95% CO2 (95C, n = 24). The time at which the gondola started descending into the well pre-filled with 80C or 95C was marked as T = 0. The CO2 exposure lasted 346 s after which the corneal reflex and breathing were assessed for 1 min. Visual assessment of changes in the amplitude and frequency of EEG traces after T = 0 was used to determine loss of consciousness. Time to loss of consciousness was longer in 80C pigs (47 ± 6 s) than in 95C pigs (33 ± 7 s). Time to an iso-electric EEG was similar in 80C pigs (75 ± 23 s) and 95C pigs (64 ± 32 s). When pigs descended into the well, the earlier entry of 95C pigs into high CO2 atmosphere rather than the concentration of CO2 by itself affected the latency of behavioral responses and decreasing brain activity. During exposure to the gas, 80C and 95C pigs exhibited sniffing, retreat attempts, lateral head movements, jumping, and gasping before loss of consciousness. 95C pigs exhibited all these behaviors on average earlier than 80C pigs after T = 0. But the interval between onset of these behaviors and loss of consciousness and the duration of these behaviors, except gasping, was similar for both treatments. Loss of posture was on average observed in both groups 10 s before EEG-based loss of consciousness. Furthermore, 88% of 80C pigs and 94% of 95C pigs

  13. Triple-reassortant influenza A virus with H3 of human seasonal origin, NA of swine origin, and internal A(H1N1) pandemic 2009 genes is established in Danish pigs.

    PubMed

    Krog, Jesper Schak; Hjulsager, Charlotte Kristiane; Larsen, Michael Albin; Larsen, Lars Erik

    2017-02-28

    This report describes a triple-reassortant influenza A virus with a HA that resembles H3 of human seasonal influenza from 2004 to 2005, N2 from influenza A virus already established in swine, and the internal gene cassette from A(H1N1)pdm09 has spread in Danish pig herds. The virus has been detected in several Danish pig herds during the last 2-3 years and may possess a challenge for human as well as animal health.

  14. No Clear Effect of Initiating Vaccination against Common Endemic Infections on the Amounts of Prescribed Antimicrobials for Danish Weaner and Finishing Pigs during 2007–2013

    PubMed Central

    Kruse, Amanda Brinch; de Knegt, Leonardo Víctor; Nielsen, Liza Rosenbaum; Alban, Lis

    2017-01-01

    It is often stated that vaccines may help reduce antimicrobial use in swine production. However, limited evidence is available outside clinical trials. We studied the change in amounts of antimicrobials prescribed for weaners and finishers in herds following initiation of vaccination against five common endemic infections: Mycoplasma hyopneumoniae, Actinobacillus pleuropneumoniae, porcine circovirus type II, porcine reproductive and respiratory syndrome virus, and Lawsonia intracellularis. Comparison was made to the change after a randomly selected date in herds not vaccinating against each of the infections. Danish sow herds initiating vaccination during 2007–2013 were included (69–334 herds, depending on the analysis). Danish sow herds with no use of the vaccine in question were included as non-exposed herds (130–570 herds, depending on the analysis). Antimicrobial prescriptions for weaners in sow herds and for finishers in receiving herds were extracted from the VetStat database for a period of 12 months before and 6–18 months after the first purchase of vaccine, or random date and quantified as average animal daily doses (ADDs) per 100 animals per day. The herd-level difference between ADD in the period after and before vaccination was the outcome in linear regression models for weaner pigs, and linear mixed-effects models for finishing pigs, taking into account sow herds delivering pigs to two or more finisher herds. Three plausible risk factors (Baseline ADD, purchase of specific vaccine, purchase of other vaccines) and five confounders (herd size, export and herd health status, year and season) were initially considered in all 10 models. The main significant effect in all models was the Baseline ADD; the higher the Baseline ADD was for weaner and finishing pigs, the larger the decrease in ADD was following vaccination (or random date for non-vaccinating herds). Regardless of vaccination status, almost equal proportions of herds experienced a

  15. A high prevalence of antimicrobial resistant Escherichia coli isolated from pigs and a low prevalence of antimicrobial resistant E. coli from cattle and sheep in Great Britain at slaughter.

    PubMed

    Enne, Virve I; Cassar, Claire; Sprigings, Katherine; Woodward, Martin J; Bennett, Peter M

    2008-01-01

    The incidence of antimicrobial resistance and expressed and unexpressed resistance genes among commensal Escherichia coli isolated from healthy farm animals at slaughter in Great Britain was investigated. The prevalence of antimicrobial resistance among the isolates varied according to the animal species; of 836 isolates from cattle tested only 5.7% were resistant to one or more antimicrobials, while only 3.0% of 836 isolates from sheep were resistant to one or more agents. However, 92.1% of 2480 isolates from pigs were resistant to at least one antimicrobial. Among isolates from pigs, resistance to some antimicrobials such as tetracycline (78.7%), sulphonamide (66.9%) and streptomycin (37.5%) was found to be common, but relatively rare to other agents such as amikacin (0.1%), ceftazidime (0.1%) and coamoxiclav (0.2%). The isolates had a diverse range of resistance gene profiles, with tet(B), sul2 and strAB identified most frequently. Seven out of 615 isolates investigated carried unexpressed resistance genes. One trimethoprim-susceptible isolate carried a complete dfrA17 gene but lacked a promoter for it. However, in the remaining six streptomycin-susceptible isolates, one of which carried strAB while the others carried aadA, no mutations or deletions in gene or promoter sequences were identified to account for susceptibility. The data indicate that antimicrobial resistance in E. coli of animal origin is due to a broad range of acquired genes.

  16. Characterisation of recently emerged multiple antibiotic-resistant Salmonella enterica serovar typhimurium DT104 and other multiresistant phage types from Danish pig herds.

    PubMed

    Baggesen, D L; Aarestrup, F M

    1998-07-25

    A total of 670 isolates of Salmonella enterica were isolated from Danish pig herds, phage typed and tested for susceptibility to amoxycillin + clavulanate, ampicillin, colistin, enrofloxacin, gentamicin, neomycin, spectinomycin, streptomycin, tetracyclines, and trimethoprim + sulphadiazine. S enterica serovar typhimurium (S typhimurium) isolates resistant to ampicillin, streptomycin and tetracycline and three isolates of S typhimurium DT104, two from 1994 and one from 1995, were further tested for resistance against chloramphenicol and sulphonamide and analysed by pulsed-field gel electrophoresis (PFGE) using the restriction enzyme Xba I. Overall, 66 per cent of the 670 isolates were sensitive to all the antimicrobial agents tested. Eleven isolates of S typhimurium were resistant to ampicillin, streptomycin and tetracycline and also resistant to other antibiotics in different resistance patterns. Seven different multiresistant clones were identified. The most common clones were four isolates of DT104 and three isolates of DT193. Two of the three S typhimurium DT104 from 1994 and 1995 were sensitive to all the antimicrobials tested whereas the remaining isolate from 1994 was resistant to spectinomycin, streptomycin and sulphonamides. All three isolates showed PFGF profiles identical to the four multiresistant DT104 isolates. Compared with most other countries antimicrobial resistance among S enterica isolated from Danish pig herds is uncommon. However, several different multiresistant clones were found.

  17. Cryptosporidium and Giardia in Danish organic pig farms: Seasonal and age-related variation in prevalence, infection intensity and species/genotypes.

    PubMed

    Petersen, Heidi H; Jianmin, Wang; Katakam, Kiran K; Mejer, Helena; Thamsborg, Stig M; Dalsgaard, Anders; Olsen, Annette; Enemark, Heidi L

    2015-11-30

    Cryptosporidium. Likewise, pigs infected with Cryptosporidium scrofarum excreted fewer oocysts (mean CPG: 54,848 ± 194,508CI: 9085-118,781) compared to pigs infected with Cryptosporidium suis (mean OPG: 351,035 ± 351,035CI: 67,953-634,117). No correlation between faecal consistency and (oo-)cyst excretion levels was observed. Of the successfully genotyped isolates, 38/56 (67.9%) were C. scrofarum and 18/56 (32.1%) were C. suis, while the livestock specific G. duodenalis Assemblage E was detected in 11/13 (84.6%) isolates and the potentially zoonotic Assemblage A was identified in 2/13 (15.4%) isolates. Piglets exclusively hosted C. suis, with one exception, while starter pigs and fatteners predominantly hosted C. scrofarum. As organic pigs are partly reared outdoors, environmental contamination with Cryptosporidium and Giardia is inevitable. Nevertheless, the present data indicate that the potential public health risk associated with both of these parasites in Danish organic pig production seems to be negligible.

  18. Pre-slaughter handling and pork quality.

    PubMed

    Vermeulen, L; Van de Perre, V; Permentier, L; De Bie, S; Verbeke, G; Geers, R

    2015-02-01

    Environmental variables, as sound levels, were collected during the pre-slaughter process in 18 different Belgian commercial slaughterhouses. Four pre-slaughter phases were determined: firstly after arrival of the truck at the slaughterhouse and just before unloading, secondly during unloading, thirdly at lairage and finally while moving to the stunner. A total of 8508 pigs was examined during the pre-slaughter process, of which the pH(LT) (M. longissimus thoracis), at 30 min post-mortem was measured. For each pre-slaughter phase, variables which might influence pork quality were determined. Moreover, this study made it possible to infer a checklist to represent and predict PSE traits of pork for all kind of pre-slaughter situations. The checklist shows also that the impact on pork quality is more decisive for the variables measured close to the stunning phase. Hence, this information is useful for the industry to optimize handling of pigs, reducing the risk for PSE traits.

  19. Microencapsulated sorbic acid and pure botanicals affect Salmonella Typhimurium shedding in pigs: a close-up look from weaning to slaughter in controlled and field conditions

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The aim of this study was to assess the efficacy of a combination of sorbic acid, thymol, and carvacrol in reducing the prevalence and shedding of Salmonella Typhimurium in pigs either in a controlled challenge environment or in a production setting. In the first study, 24 weaned piglets were dist...

  20. Assessment of human health risk associated with pyaemia in Danish finisher pigs when conducting visual-only inspection of the lungs.

    PubMed

    Kruse, Amanda Brinch; Larsen, Marianne Halberg; Skou, Peter Bæk; Alban, Lis

    2015-03-02

    The most important lesion to be overlooked when performing visual-only inspection of the lungs is embolic pneumonia. The aim of the present study was to assess the additional human health risk represented by overlooking cases of pyaemia represented by embolic pneumonia in finisher pigs, when conducting visual-only compared to palpation of the lungs, as is the traditional meat inspection procedure. An examination of bacteria isolated from 19 finisher pigs identified with embolic pneumonia at traditional meat inspection was undertaken. From each pig samples were taken from various organs (lungs, spleen, heart, liver and kidney), from the carpal joints (A. carpi) and flexor muscle (M. flexor digitorum superficialis) on the right foreleg. These data were included in a risk assessment following OIE guidelines. Bacteria were isolated from 78 out of 127 tissue and swap samples taken (61% positive samples). Staphylococcus aureus (N=37) was the most frequently isolated bacterium. The predominant site of S. aureus was the lung. S. aureus was detected although less frequently in low numbers in some organs (<100CFU/sample) and muscle samples (<10CFU/sample). Only one MRSA isolate was found. Staphylococcus warneri (N=24) was the second most commonly found bacterium. There was no predominant site and the number of S. warneri was less than 50CFU per sample. The risk of a food-borne intoxication from S. aureus in relation to pyaemia in pigs was considered very low due to the low quantitative numbers of S. aureus in muscle tissue samples. Implementing visual-only inspection will reduce the exposure of S. aureus due to less cross-contamination and handling of the plucks by the meat inspectors. The human health risk associated with S. warneri was considered very low, due to the limited zoonotic potential of this bacterium. In conclusion, the additional human health risk in relation to possibly overlooking pyaemia in Danish finisher pigs was considered negligible when conducting

  1. Oxidative and colour changes in meat from three lines of free-range reared Iberian pigs slaughtered at 90 kg live weight and from industrial pig during refrigerated storage.

    PubMed

    Estévez, Mario; Morcuende, David; Cava, Ramón

    2003-11-01

    The oxidative stability and colour evolution during refrigerated storage (10 days at +4 °C) of Longissimus dorsi muscle from three lines of Iberian pigs and industrial genotype pigs were studied. The Iberian pigs were reared in an extensive system and fed on a commercial feed with access to pasture grazing, while industrial genotype pigs were reared in an intensive system. Fresh Longissimus dorsi of Iberian pig lines showed a more intense and redder colour, with a statistically significant higher CIE chroma and a*-value as well as a lower L*-value (P<0.05) compared with those of industrial genotype pigs. Pig genotype significantly affected the colour and lipid oxidation changes of m. Longissimus dorsi during refrigerated storage. The decrease in L*-value during the refrigerated storage was more intense in industrial pig's Longissimus dorsi than in those from Iberian pig lines. CIE a*-value decrease was more intense in 'Lampiño' Iberian line and industrial Longissimus dorsi than in the other two Iberian pig lines, however CIE a*-value was significantly higher in muscles from Iberian pig lines than in muscle of industrial genotype (P<0.05) at the end of refrigerated storage. The Iberian and industrial pig's Longissimus dorsi did not show statistically significant differences in TBA-RS values (mg MDA/kg meat) at the end of the refrigerated storage period (P>0.05). To establish relationships between parameters measured in fresh meat and oxidation and colour changes during refrigerated storage, correlations and multiple regression models were calculated. CIE a*-values at day 10 were positively correlated with values of fresh muscles for a*, C18:1 percentages and negatively correlated with polyunsaturated fatty acids percentages of neutral and polar lipids. Inversely, TBA-RS values at day 10 showed a positive correlation with initial values for phospholipid content and polyunsaturated fatty acids of neutral and polar lipid fractions, while C18:1 percentage were

  2. Growth performance and pork fat quality as measured by three methods of pigs fed 20% DDGS and slaughtered using a standard industry split marketing strategy.

    PubMed

    Shircliff, K E; Callahan, Z D; Wilmoth, T A; Ohman, C E; Johnson, R C; Wiegand, B R

    2015-10-01

    A total of 40 pens containing 22 crossbred barrows (initial BW = 43.07 ± 1.61 kg; PIC 1050 × PIC 337 genetics) were housed in a commercial wean to finish facility. Pens were randomly allotted to dietary treatments in a 2 × 3 factorial arrangement with 2 levels of dried distillers grains with solubles (DDGS; 0% or 20%) and chosen for 1 of 3 marketing cuts removing 4, 8, and 10 animals from each pen. Fat tissue samples were removed from the anterior tip of the jowl and posterior to the sternum on the belly edge 1d postmortem. Fatty acid composition was determined via the Folch method, and iodine values (IV) were calculated from chemical titrations, fatty acid profile (GC IV), and in-plant Bruker near-infrared (NIR) spectroscopy. Pearson's correlation coefficients for IV determination methods were estimated. Inclusion of 20% DDGS did not change ( > 0.05) growth performance, whereas marketing cut affected performance, with the second cut producing the most efficient pigs ( < 0.01). Total SFA and MUFA concentrations were higher ( < 0.01) in belly and jowl fat from pigs fed 0% DDGS. Total PUFA and the PUFA:SFA in belly and jowl fat was higher ( < 0.01) when 20% DDGS was fed. Dried distillers grains with solubles inclusion increased IV in belly and jowl as determined by all 3 methods. Regardless of dietary treatment or fat depot, Pearson correlation coefficients between titration and GC IV, titration and NIR, and GC IV and NIR were 0.46 ( < 0.01), 0.68 ( < 0.01), and 0.43 ( < 0.01), respectively. These correlations suggest methods may rank samples equally but do not provide the same absolute IV. Belly fat had a lower IV ( < 0.01) than jowl fat using titration or GC IV methods, suggesting pigs have varied degrees of physiological maturity at specific fat depots when weight end points are used during the finishing phase. In conclusion, feeding 20% DDGS negatively affected fat quality but not growth performance, and marketing time changed growth performance.

  3. The effect of discontinued use of antimicrobial growth promoters on the risk of therapeutic antibiotic treatment in Danish farrow-to-finish pig farms.

    PubMed

    Vigre, H; Larsen, P B; Andreasen, M; Christensen, J; Jorsal, S E

    2008-01-01

    This study estimated the effect of discontinued use of antimicrobial growth promoters (duAGPs) on the risk of antibiotic treatment for diarrhoea, arthritis, pneumonia, unthriving and miscellaneous disorders in Danish pig farms. The estimation was done in a case-crossover study comparing: (1) the proportion of days per farm where treatment was performed (PDT) and (2) the proportion of pigs treated per day per farm at days where treatment was performed (PPT) before and after duAGPs at 68 farrow-to-finish farms. The farms were selected using a two-stage (veterinarian/farm) convenience sampling. On average, during the first year after duAGPs there was a significant increase in the risk of antibiotic treatment for diarrhoea (PDT: OR 2.5, 95% CI 1.7-3.8; PPT: OR 1.6, 95% CI 1.1-2.2). However, the effect varied among farms--some farms experienced substantial problems, while others experienced few problems after duAGPs. No effect was identified for the risk of treatment for other diseases.

  4. Resistance phenotypes and genotypes among multiple-antimicrobial-resistant Salmonella enterica subspecies enterica serovar Choleraesuis strains isolated between 2008 and 2012 from slaughter pigs in Okinawa Prefecture, Japan

    PubMed Central

    MATAYOSHI, Masanao; KITANO, Takashi; SASAKI, Tetsu; NAKAMURA, Masaji

    2015-01-01

    A total of 349 Salmonella enterica subspecies enterica serovar Choleraesuis (S. Choleraesuis) strains, which were isolated between 2008 and 2012 from 349 pigs at two slaughterhouses in Okinawa Prefecture, Japan, were investigated for antimicrobial susceptibility and the presence of antimicrobial resistance genes. All isolates were resistant to at least four antimicrobial agents. The antimicrobial agents for which isolates showed a high incidence of resistance were as follows: ampicillin (100%) and streptomycin (100%), followed by gentamicin (99.7%), oxytetracycline (99.7%), sulfamethoxazole/trimethoprim (99.4%), nalidixic acid (40.1%) and oxolinic acid (40.1%). All isolates were sensitive to cefuroxime, ceftiofur, colistin, fosfomycin, enrofloxacin, orbifloxacin and danofloxacin. The predominant resistance phenotypes and genotypes were: resistance to ampicillin, streptomycin, gentamicin, oxytetracycline and sulfamethoxazole/trimethoprim (58.5%, 204/349) and blaTEM-strA-strB-aadA1-aadA2-aacC2-tet (B)-sul1-sul2-dhfrXII-dhfrXIII (36.1%, 126/349). The quinolone resistance-determining regions (QRDRs) of gyrA, gyrB, parC and parE of the quinolone-resistant isolates (n=12) showed amino acid substitutions of Ser-83→Phe or Asp-87→Tyr in GyrA and Ser-107→Ala in ParC. To our knowledge, this is the first report on the molecular characterization of antimicrobial resistance among S. Choleraesuis strains in Japan. PMID:25715779

  5. Resistance phenotypes and genotypes among multiple-antimicrobial-resistant Salmonella enterica subspecies enterica serovar Choleraesuis strains isolated between 2008 and 2012 from slaughter pigs in Okinawa Prefecture, Japan.

    PubMed

    Matayoshi, Masanao; Kitano, Takashi; Sasaki, Tetsu; Nakamura, Masaji

    2015-06-01

    A total of 349 Salmonella enterica subspecies enterica serovar Choleraesuis (S. Choleraesuis) strains, which were isolated between 2008 and 2012 from 349 pigs at two slaughterhouses in Okinawa Prefecture, Japan, were investigated for antimicrobial susceptibility and the presence of antimicrobial resistance genes. All isolates were resistant to at least four antimicrobial agents. The antimicrobial agents for which isolates showed a high incidence of resistance were as follows: ampicillin (100%) and streptomycin (100%), followed by gentamicin (99.7%), oxytetracycline (99.7%), sulfamethoxazole/trimethoprim (99.4%), nalidixic acid (40.1%) and oxolinic acid (40.1%). All isolates were sensitive to cefuroxime, ceftiofur, colistin, fosfomycin, enrofloxacin, orbifloxacin and danofloxacin. The predominant resistance phenotypes and genotypes were: resistance to ampicillin, streptomycin, gentamicin, oxytetracycline and sulfamethoxazole/trimethoprim (58.5%, 204/349) and blaTEM-strA-strB-aadA1-aadA2-aacC2-tet (B)-sul1-sul2-dhfrXII-dhfrXIII (36.1%, 126/349). The quinolone resistance-determining regions (QRDRs) of gyrA, gyrB, parC and parE of the quinolone-resistant isolates (n=12) showed amino acid substitutions of Ser-83→Phe or Asp-87→Tyr in GyrA and Ser-107→Ala in ParC. To our knowledge, this is the first report on the molecular characterization of antimicrobial resistance among S. Choleraesuis strains in Japan.

  6. Genetic parameters between slaughter pig efficiency and growth rate of different body tissues estimated by computed tomography in live boars of Landrace and Duroc.

    PubMed

    Gjerlaug-Enger, E; Kongsro, J; Odegård, J; Aass, L; Vangen, O

    2012-01-01

    In this study, computed tomography (CT) technology was used to measure body composition on live pigs for breeding purposes. Norwegian Landrace (L; n = 3835) and Duroc (D; n = 3139) boars, selection candidates to be elite boars in a breeding programme, were CT-scanned between August 2008 and August 2010 as part of an ongoing testing programme at Norsvin's boar test station. Genetic parameters in the growth rate of muscle (MG), carcass fat (FG), bone (BG) and non-carcass tissue (NCG), from birth to ∼100 kg live weight, were calculated from CT data. Genetic correlations between growth of different body tissues scanned using CT, lean meat percentage (LMP) calculated from CT and more traditional production traits such as the average daily gain (ADG) from birth to 25 kg (ADG1), the ADG from 25 kg to 100 kg (ADG2) and the feed conversion ratio (FCR) from 25 kg to 100 kg were also estimated from data on the same boars. Genetic parameters were estimated based on multi-trait animal models using the average information-restricted maximum likelihood (AI-REML) methodology. The heritability estimates (s.e. = 0.04 to 0.05) for the various traits for Landrace and Duroc were as follows: MG (0.19 and 0.43), FG (0.53 and 0.59), BG (0.37 and 0.58), NCG (0.38 and 0.50), LMP (0.50 and 0.57), ADG1 (0.25 and 0.48), ADG2 (0.41 and 0.42) and FCR (0.29 and 0.42). Genetic correlations for MG with LMP were 0.55 and 0.68, and genetic correlations between MG and ADG2 were -0.06 and 0.07 for Landrace and Duroc, respectively. LMP and ADG2 were clearly unfavourably genetically correlated (L: -0.75 and D: -0.54). These results showed the difficulty in jointly improving LMP and ADG2. ADG2 was unfavourably correlated with FG (L: 0.84 and D: 0.72), thus indicating to a large extent that selection for increased growth implies selection for fatness under an ad libitum feeding regime. Selection for MG is not expected to increase ADG2, but will yield faster growth of the desired tissues and a better

  7. Pre-slaughter rectal temperature as an indicator of pork meat quality.

    PubMed

    Vermeulen, L; Van de Perre, V; Permentier, L; De Bie, S; Geers, R

    2015-07-01

    This study investigates whether rectal temperature of pigs, prior to slaughter, can give an indication of the risk of developing pork with PSE characteristics. A total of 1203 pigs were examined, measuring the rectal temperature just before stunning, of which 794 rectal temperatures were measured immediately after stunning. pH30LT (M. Longissimus thoracis) and temperature of the ham (Temp30Ham) were collected from about 530 carcasses, 30 min after sticking. The results present a significant positive linear correlation between rectal temperature just before and after slaughter, and Temp30Ham. Moreover, pH30LT is negatively correlated with rectal temperature and Temp30Ham. Finally, a linear mixed model for pH30LT was established with the rectal temperature of the pigs just before stunning and the lairage time. This model defines that measuring rectal temperature of pigs just before slaughter allows discovery of pork with PSE traits, taking into account pre-slaughter conditions.

  8. Salmonella enterica prevalence and serotype distribution in swine at slaughter

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The objective of this cross-sectional study was to analyze data available from multiple studies conducted by our research team estimating the prevalence of S. enterica, and the serotype distribution in swine at slaughter, based on different sample types. A total of 1,110 pigs from three large capaci...

  9. Hazard identification in swine slaughter with respect to foodborne bacteria.

    PubMed

    Borch, E; Nesbakken, T; Christensen, H

    1996-06-01

    Swine slaughter is an open process with many opportunities for the contamination of the pork carcass with potentially pathogenic bacteria; however, it does not contain any point where hazards are completely eliminated. Data on the prevalence of various pathogenic bacteria (Aeromonas hydrophila, Campylobacter coli/jejuni, Listeria monocytogenes, Salmonella spp., Staphylococcus aureus and Yersinia enterocolitica) in pigs, their growth and survival characteristics and ability to become established on the slaughter line are presented. The presentation covers the processing steps from lairage to chilling and is based on swine slaughter practices in Denmark, Norway and Sweden. The major contamination points during swine slaughter are pig-related, such as faecal and pharyngeal, and environmental. HACCP (Hazard Analysis Critical Control Point) and GMP (Good Manufacturing Practice) in swine slaughter must be focused on limiting this spread. The pathogenic bacteria show differences in their general mechanism of distribution. The major contamination source of Campylobacter spp., Salmonella spp. and Y. enterocolitica is the pig, and the contamination of carcasses with these bacteria may be limited, provided that only strict slaughtering procedures are used. Other organisms such as Aeromonas spp., L. moncytogenes/Listeria spp. and S. aureus can be endemic in the processing environment. Since endemic bacteria can be controlled by proper cleaning and disinfection, these organisms are useful as indicators for the success of GMP rules. The following affiliation to CPs or CCPs made for specific steps during slaughter and dressing may serve as a guidance: (i) lairage (CP), (ii) killing (CP), (iii) scalding (CP), (iv) dehairing (CP), (v) singeing/flaming (CP), (vi) polishing (CP), (vii) circumanal incision and removal of the intestines (CCP), (viii) excision of the tongue, pharynx, and in particular the tonsils (CCP), (ix) splitting (CP), (x) post mortem inspection procedures (CCP

  10. Role of slaughtering in Salmonella spreading and control in pork production.

    PubMed

    Arguello, Hector; Alvarez-Ordoñez, Avelinó; Carvajal, Ana; Rubio, Pedro; Prieto, Miguel

    2013-05-01

    Salmonella is one of the major foodborne pathogens worldwide. Pork products are among the main sources of Salmonella infection in humans, and several countries have established Salmonella surveillance and control programs. The role of slaughtering in carcass contamination has been indicated by studies focused on the slaughterhouse environment. In this review, we examine and discuss the information available regarding the influence that farm status, pig transport, and lairage have on the carriage of Salmonella by pigs entering the slaughter line. The evolution of carcass contamination throughout the slaughtering process, the main sources of contamination in the dirty and clean zones of the slaughter line, and previously reported prevalence of Salmonella on carcasses and factors affecting this prevalence also are discussed. The importance of implementing interventions at the slaughter level is discussed briefly. Consistent with the information available, pigs from infected farms and newly acquired or recrudescent infections in pigs at the subsequent stages of transport and lairage are important sources of Salmonella at the slaughtering plant. The continuous introduction of Salmonella into the slaughterhouse and the potential for resident flora constitute a risk for carcass contamination. At the slaughterhouse, some dressing activities can reduce carcass contamination, but others are critical control points that jeopardize carcass hygiene. This information indicates the importance of considering slaughter and previous stages in the pork production chain for controlling Salmonella in swine production.

  11. Salmonella control measures with special focus on vaccination and logistic slaughter procedures.

    PubMed

    Hotes, S; Traulsen, I; Krieter, J

    2011-10-01

    This study focussed on the effectiveness of Salmonella control measures to decrease Salmonella prevalence at slaughter. Considered measures were the control of hygiene and husbandry management as well as vaccination and logistic slaughter procedures. Results emphasized the capabilities of the farrowing stage to influence slaughter pig prevalence. Limited Salmonella entry by the implementation of hygiene control measures at farrowing farms obtained a significant decrease in prevalence after lairage at slaughterhouse. In contrast, hygiene control measures at finishing stage were less effective. Husbandry control measures, preventing physical contacts between pigs, were proved to decrease slaughter pig prevalence whether they were implemented at farrowing or finishing stage. Furthermore, the vaccination of sows and piglets was an appropriate control measure to decrease slaughter pig prevalence, if a large part of farms established this control measure. Simultaneous implementation of control measures showed that vaccination and especially hygiene measures are mutually supportive. Concerning logistic slaughter procedures it became obvious that with decreasing prevalence, infections at transport and lairage become more and more important. The herd status separation significantly decreased the percentage of infected pigs that became infected at lairage.

  12. Pre-slaughter sound levels and pre-slaughter handling from loading at the farm till slaughter influence pork quality.

    PubMed

    Vermeulen, L; Van de Perre, V; Permentier, L; De Bie, S; Verbeke, G; Geers, R

    2016-06-01

    This study investigates the relationship between sound levels, pre-slaughter handling during loading and pork quality. Pre-slaughter variables were investigated from loading till slaughter. A total of 3213 pigs were measured 30 min post-mortem for pH(30LT) (M. Longissimus thoracis). First, a sound level model for the risk to develop PSE meat was established. The difference in maximum and mean sound level during loading, mean sound level during lairage and mean sound level prior to stunning remained significant within the model. This indicated that sound levels during loading had a significant added value to former sound models. Moreover, this study completed the global classification checklist (Vermeulen et al., 2015a) by developing a linear mixed model for pH(30LT) and PSE prevalence, with the difference in maximum and mean sound level measured during loading, the feed withdrawal period and the difference in temperature during loading and lairage. Hence, this study provided new insights over previous research where loading procedures were not included.

  13. The dose-response relationship between the amount of straw provided on the floor and gastric ulceration of pars oesophagea in growing pigs.

    PubMed

    Jensen, Karin H; Jørgensen, Lisbeth; Haugegaard, Svend; Herskin, Mette S; Jensen, Margit B; Pedersen, Lene J; Canibe, Nuria

    2017-01-12

    The aim of the present study was (1) to determine the dose-response relationship between the amount of straw provided on the floor and oesophageal ulceration in pigs kept under typical Danish production conditions (18 pigs/pen, 0.7m(2)/pig, partly slatted floor, ad libitum access to feed), (2) to reveal whether straw ingestion explains the effect of straw provision on the stomach health and (3) to elucidate the effect of straw ingestion on the stomach conditions. Data were collected at slaughter (approximately 100kg body weight) on pigs provided with straw amounts in the range 10g to 500g wheat straw/pig/day from 30kg body weight and fed a wheat-based pelleted feed added 15% non-heated and non-pelleted rolled barley. Aims (1) and (2) included 712 pigs kept in 42 pens, whereas (3) was studied on a subset of 37 pigs with either none or obvious amounts of straw in the stomach. The amount of straw provided affected stomach health in a curvilinear manner. Provision of up to approximately 300gstraw/pig/day progressively decreased the risk of oesophageal ulceration and scarring. At larger amounts of straw the ulceroprotective effect of straw was reduced which requires further investigation. Straw ingestion appeared to be an essential intermediary factor for the improvement of stomach health. The number of pigs without straw in the stomach decreased linearly with the logarithm of the amount of straw provided, which explained the effect of straw provision on stomach health. The structure of the stomach contents increased as revealed by the increased weight and dry matter content and decreased sedimentation of the digesta. Provision of approximately 300gstraw/pig/day on the floor may be a potential strategy to reduce, but not inevitably eliminate, oesophageal ulceration in pigs in commercial pig production.

  14. Pig Feeding under the Potato-green Forage Base System with or without Addition of Herbs versus a Concentrate Based System: Effect on Post-slaughter Performance and Pork Characteristics

    PubMed Central

    Turyk, Zofia; Osek, Maria; Olkowski, Bogusław; Janocha, Alina

    2014-01-01

    This study examined carcass and meat quality parameters in growing/finishing pigs fed unconventionally versus the concentrate-based system. Ninety-six, 12 wk old pigs (34±SD 0.3 kg) were randomly divided into three groups, assigned to one of the three dietary treatments: standard complete concentrate mixture, conventional (C diet); unconventional, steamed potato-green forage-concentrate based diet (U diet), and unconventional basal diet+herbage mix (UH diet). Pigs fed U diet showed lower dressing percentage, meatiness, loin eye area, and weight of pork neck (p≤0.05), but their carcasses were significantly (p≤0.05) longer and had increased backfat depth (p≤0.05). There was no impact of the diet on the meat content of dry matter, crude ash, acidity, and colour parameters of m. longissimus. Unconventional feeding significantly (p≤0.05) elevated water the holding capacity of m. longissimus and slightly improved the sensory attributes analysis of meat. The addition of herbs resulted in increased loin eye area (p≤0.05), decreased fat content (p≤0.05) in m. longissimus, and tended to improve some sensory attributes of meat. There were significant gender differences in response to all diets. There were significant diet×sex interactions for some measured variables, but there were no clearly identifiable trends with regard to any specific carcass or meat parameters. Feeding unconventional diet to pigs may offer better culinary attributes of the meat, and improve some technologically important characteristics of pig carcass, but may negatively affect some carcass or meat parameters. PMID:25050003

  15. Pig Feeding under the Potato-green Forage Base System with or without Addition of Herbs versus a Concentrate Based System: Effect on Post-slaughter Performance and Pork Characteristics.

    PubMed

    Turyk, Zofia; Osek, Maria; Olkowski, Bogusław; Janocha, Alina

    2014-05-01

    This study examined carcass and meat quality parameters in growing/finishing pigs fed unconventionally versus the concentrate-based system. Ninety-six, 12 wk old pigs (34±SD 0.3 kg) were randomly divided into three groups, assigned to one of the three dietary treatments: standard complete concentrate mixture, conventional (C diet); unconventional, steamed potato-green forage-concentrate based diet (U diet), and unconventional basal diet+herbage mix (UH diet). Pigs fed U diet showed lower dressing percentage, meatiness, loin eye area, and weight of pork neck (p≤0.05), but their carcasses were significantly (p≤0.05) longer and had increased backfat depth (p≤0.05). There was no impact of the diet on the meat content of dry matter, crude ash, acidity, and colour parameters of m. longissimus. Unconventional feeding significantly (p≤0.05) elevated water the holding capacity of m. longissimus and slightly improved the sensory attributes analysis of meat. The addition of herbs resulted in increased loin eye area (p≤0.05), decreased fat content (p≤0.05) in m. longissimus, and tended to improve some sensory attributes of meat. There were significant gender differences in response to all diets. There were significant diet×sex interactions for some measured variables, but there were no clearly identifiable trends with regard to any specific carcass or meat parameters. Feeding unconventional diet to pigs may offer better culinary attributes of the meat, and improve some technologically important characteristics of pig carcass, but may negatively affect some carcass or meat parameters.

  16. 7 CFR 65.250 - Slaughter.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 3 2014-01-01 2014-01-01 false Slaughter. 65.250 Section 65.250 Agriculture... OF BEEF, PORK, LAMB, CHICKEN, GOAT MEAT, PERISHABLE AGRICULTURAL COMMODITIES, MACADAMIA NUTS, PECANS, PEANUTS, AND GINSENG General Provisions Definitions § 65.250 Slaughter. Slaughter means the point in...

  17. 7 CFR 65.250 - Slaughter.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 3 2011-01-01 2011-01-01 false Slaughter. 65.250 Section 65.250 Agriculture... OF BEEF, PORK, LAMB, CHICKEN, GOAT MEAT, PERISHABLE AGRICULTURAL COMMODITIES, MACADAMIA NUTS, PECANS, PEANUTS, AND GINSENG General Provisions Definitions § 65.250 Slaughter. Slaughter means the point in...

  18. 7 CFR 65.250 - Slaughter.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Slaughter. 65.250 Section 65.250 Agriculture... OF BEEF, PORK, LAMB, CHICKEN, GOAT MEAT, PERISHABLE AGRICULTURAL COMMODITIES, MACADAMIA NUTS, PECANS, PEANUTS, AND GINSENG General Provisions Definitions § 65.250 Slaughter. Slaughter means the point in...

  19. 7 CFR 65.250 - Slaughter.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 3 2012-01-01 2012-01-01 false Slaughter. 65.250 Section 65.250 Agriculture... OF BEEF, PORK, LAMB, CHICKEN, GOAT MEAT, PERISHABLE AGRICULTURAL COMMODITIES, MACADAMIA NUTS, PECANS, PEANUTS, AND GINSENG General Provisions Definitions § 65.250 Slaughter. Slaughter means the point in...

  20. 7 CFR 65.250 - Slaughter.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 3 2013-01-01 2013-01-01 false Slaughter. 65.250 Section 65.250 Agriculture... OF BEEF, PORK, LAMB, CHICKEN, GOAT MEAT, PERISHABLE AGRICULTURAL COMMODITIES, MACADAMIA NUTS, PECANS, PEANUTS, AND GINSENG General Provisions Definitions § 65.250 Slaughter. Slaughter means the point in...

  1. A two-nucleotide deletion renders the mannose-binding lectin 2 (MBL2) gene nonfunctional in Danish Landrace and Duroc pigs.

    PubMed

    Bergman, I M; Edman, K; van As, P; Huisman, A; Juul-Madsen, Helle Risdahl

    2014-03-01

    The mannose-binding lectins (MBLs) are central components of innate immunity, facilitating phagocytosis and inducing the lectin activation pathway of the complement system. Previously, it has been found that certain single-nucleotide polymorphisms (SNPs) in porcine MBL1 and MBL2 (pMBL1, pMBL2) affect mRNA expression, serum concentration, and susceptibility to disease, but the combinatory effect of pMBL1 and pMBL2 genotypes needs further elucidation. In the present study, pMBL1 and pMBL2 alleles, combined pMBL haplotypes, and MBL-A concentration in serum were analyzed in purebred Landrace (N = 30) and Duroc (N = 10) pigs. Furthermore, the combined pMBL haplotypes of 89 Piètrain × (Large White × Landrace) crossbred pigs were studied, and the genotypes of 67 crossbreds challenged with Escherichia coli were compared to their individual disease records. In the purebred animals, three non-synonymous SNPs and a two-nucleotide deletion were detected in the coding sequence of pMBL2. The two-nucleotide deletion was present at a frequency of 0.88 in the Landrace pigs and 0.90 in the Duroc pigs, respectively. In the crossbreds, the T allele of the SNP G949T in pMBL1-previously shown to have profound effect on MBL-A concentration even in the heterozygote condition-was detected in 47 % of the animals. Finally, an association was found between low-producing MBL genotypes and low body weight on the day of weaning in the same animals.

  2. Investigation of the disposal of dead pigs by pig farmers in mainland China by simulation experiment.

    PubMed

    Wu, Linhai; Xu, Guoyan; Li, Qingguang; Hou, Bo; Hu, Wuyang; Wang, Jianhua

    2017-01-01

    Dead pigs are a major waste by-product of pig farming. Thus, safe disposal of dead pigs is important to the protection of consumer health and the ecological environment by preventing marketing of slaughtered and processed dead pigs and improper dumping of dead pigs. In this study, a probability model was constructed for the disposal of dead pigs by pig farmers by selecting factors affecting disposal. To that end, we drew on the definition and meaning of behavior probability based on survey data collected from 654 pig farmers in Funing County, Jiangsu Province, China. Moreover, the role of influencing factors in pig farmers' behavioral choices regarding the disposal of dead pigs was simulated by simulation experiment. The results indicated that years of farming had a positive impact on pig farmers' choice of negative disposal of dead pigs. Moreover, there was not a simple linear relationship between scale of farming and pig farmers' behavioral choices related to the disposal of dead pigs. The probability for farmers to choose the safe disposal of dead pigs increased with the improvement of their knowledge of government policies and relevant laws and regulations. Pig farmers' behavioral choice about the disposal of dead pigs was also affected by government subsidy policies, regulation, and punishment. Government regulation and punishment were more effective than subsidy. The findings of our simulation experiment provide important decision-making support for the governance in preventing the marketing of dead pigs at the source.

  3. Determining the optimal number of individual samples to pool for quantification of average herd levels of antimicrobial resistance genes in Danish pig herds using high-throughput qPCR.

    PubMed

    Clasen, Julie; Mellerup, Anders; Olsen, John Elmerdahl; Angen, Øystein; Folkesson, Anders; Halasa, Tariq; Toft, Nils; Birkegård, Anna Camilla

    2016-06-30

    The primary objective of this study was to determine the minimum number of individual fecal samples to pool together in order to obtain a representative sample for herd level quantification of antimicrobial resistance (AMR) genes in a Danish pig herd, using a novel high-throughput qPCR assay. The secondary objective was to assess the agreement between different methods of sample pooling. Quantification of AMR was achieved using a high-throughput qPCR method to quantify the levels of seven AMR genes (ermB, ermF, sulI, sulII, tet(M), tet(O) and tet(W)). A large variation in the levels of AMR genes was found between individual samples. As the number of samples in a pool increased, a decrease in sample variation was observed. It was concluded that the optimal pooling size is five samples, as an almost steady state in the variation was observed when pooling this number of samples. Good agreement between different pooling methods was found and the least time-consuming method of pooling, by transferring feces from each individual sample to a tube using a 10μl inoculation loop and adding 3.5ml of PBS, approximating a 10% solution, can therefore be used in future studies.

  4. Direct feeding of microencapsulated bacteriophages to reduce Salmonella colonization in pigs

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Salmonella shedding often increases in pigs following pre-slaughter transportation and/or lairage. We previously showed that administering anti-Salmonella bacteriophages to pigs by gavage significantly reduced Salmonella colonization when the pigs were exposed to a Salmonella-contaminated pen. In ...

  5. Comparison of fecal culture and Danish Mix-ELISA for determination of Salmonella enterica subsp. enterica prevalence in growing swine.

    PubMed

    Funk, J A; Harris, I T; Davies, P R

    2005-04-25

    In the USA, control of food-borne salmonellosis associated with meat consumption has been predominantly focused at slaughter and processing. It is expected that standards at slaughter and processing will become more stringent, creating pressure to reduce prevalence of Salmonella-positive food animals through on-farm interventions. The aim of this study was to compare traditional fecal culture and the Danish Mix-ELISA (DME) for determination of Salmonella prevalence pre-harvest in swine. In Trial 1, five cohorts of individually identified pigs were longitudinally sampled during the growing period to compare the kinetics of prevalence as estimated by fecal culture and the DME. In Trial 2, the correlation between fecal prevalence and seroprevalence was estimated pre-marketing in 49 groups of pigs. In Trial 1, fecal prevalence and seroprevalence showed similar kinetics, with a tendency of a higher OD% cut-off to more closely approximate fecal prevalence. In Trial 2, correlations between fecal culture and the DME were 0.40, 0.36, 0.43, and 0.43 (p<0.001) for OD% cut-offs > or =10, 20, 30, and 40, respectively. Based on these results, a higher OD% cut-off would be recommended if more approximate estimation of fecal prevalence is desired and longitudinal sampling would be suggested for evaluating the impact of on-farm interventions for Salmonella reduction whether utilizing fecal culture or the DME. Further evaluation of the impact of Salmonella serovar present on farms on seroprevalence and the relationship of on-farm seroprevalence with food safety risk are needed prior to utilizing the DME for pre-harvest Salmonella diagnostics in the US swine herd.

  6. A quantitative approach towards a better understanding of the dynamics of Salmonella spp. in a pork slaughter-line.

    PubMed

    van Hoek, Angela H A M; de Jonge, Rob; van Overbeek, Wendy M; Bouw, El; Pielaat, Annemarie; Smid, Joost H; Malorny, Burkhard; Junker, Ernst; Löfström, Charlotta; Pedersen, Karl; Aarts, Henk J M; Heres, Lourens

    2012-02-01

    Pork contributes significantly to the public health disease burden caused by Salmonella infections. During the slaughter process pig carcasses can become contaminated with Salmonella. Contamination at the slaughter-line is initiated by pigs carrying Salmonella on their skin or in their faeces. Another contamination route could be resident flora present on the slaughter equipment. To unravel the contribution of these two potential sources of Salmonella a quantitative study was conducted. Process equipment (belly openers and carcass splitters), faeces and carcasses (skin and cutting surfaces) along the slaughter-line were sampled at 11 sampling days spanning a period of 4 months. Most samples taken directly after killing were positive for Salmonella. On 96.6% of the skin samples Salmonella was identified, whereas a lower number of animals tested positive in their rectum (62.5%). The prevalence of Salmonella clearly declined on the carcasses at the re-work station, either on the cut section or on the skin of the carcass or both (35.9%). Throughout the sampling period of the slaughter-line the total number of Salmonella per animal was almost 2 log lower at the re-work station in comparison to directly after slaughter. Seven different serovars were identified during the study with S. Derby (41%) and S. Typhimurium (29%) as the most prominent types. A recurring S. Rissen contamination of one of the carcass splitters indicated the presence of an endemic 'house flora' in the slaughterhouse studied. On many instances several serotypes per individual sample were found. The enumeration of Salmonella and the genotyping data gave unique insight in the dynamics of transmission of this pathogen in a slaughter-line. The data of the presented study support the hypothesis that resident flora on slaughter equipment was a relevant source for contamination of pork.

  7. Stress Factors During Cattle Slaughter.

    PubMed

    Disanto, Chiara; Celano, Giuseppe; Varvara, Michele; Fusiello, Nunziana; Fransvea, Armida; Bozzo, Giancarlo; Celano, Gaetano Vitale

    2014-08-28

    Monitoring animal welfare at a slaughterhouse is somehow different from the situation on the farm. The throughput numbers can be very high, and there is no possibility to know the individual history, background or habits of each animal. The animals are unfamiliar to the staff and viceversa. Furthermore, most animals will be unknown to each other even if they may be delivered in groups or batches, and it is not rare to see mixing of completely unfamiliar animals. The slaughter plants' environment is of course completely unknown to the animals, with large areas not always designed to minimize noise or visual distraction for animals. Sub-optimal handling of animals can unfortunately be seen at many slaughterhouses, where animals are coerced roughly, using sticks or prods and loud voices, forcing the animals to move fast in the desired direction. Vets need to be aware of these factors because they can cause excessive prodding for animals, which may become balking, excited and stressed. A survey of 10 italian slaughter plants revealed that: 5 plants had slick floors that would cause animals to slip and fall, 9 plants had high pitched motor noise or hissing air that made animals balking; air drafts blowing down the chutes, which will often impede animal movement, were a problem in 9 of the observed plants. Simple modifications of lighting and elimination of air drafts and hissing will often greatly improve animal movement.

  8. Genetic parameters for male fertility and its relationship to skatole and androstenone in Danish Landrace boars.

    PubMed

    Strathe, A B; Velander, I H; Mark, T; Ostersen, T; Hansen, C; Kadarmideen, H N

    2013-10-01

    Concerns have been raised regarding selection against the boar taint compounds, androstenone and skatole, due to potential unfavorable genetic correlations with important male fertility traits (i.e., selection of boars with low levels of these boar taint compounds might also reduce male fertility). Hence, the objective of this investigation was to study the genetic association between direct measures of male fertility and the boar taint compounds in Danish Landrace pigs. Concentrations of skatole and androstenone in the back fat were available for approximately 6,000 and 1,000 Landrace boars, respectively. The litter size traits, such as total number born, live piglets at d 5, and piglet survival until d 5 on relatives of the slaughter boars, were extracted from the Danish Landrace breeding program, yielding 35,715 records. Semen volume, sperm concentration, subjective sperm quality score, and total number of sperm were available from 95,267 ejaculates. These ejaculates were collected between 2005 and 2012 and originated from 3,145 Landrace boars from 12 AI stations in Denmark. The traits were analyzed using single and multitrait animal models including univariate random regression models. Skatole and androstenone concentrations were moderate to highly heritable (i.e., 0.33 and 0.59, respectively). The genetic correlation between the two compounds was moderate (0.40). Genetic variance of sperm production per ejaculate increased during the productive life of the boar, resulting in heritability estimates increasing from 0.18 to 0.31. Genetic correlations between sperm production per ejaculate at different ages were high and generally larger than 0.8, indicating that later genetic merit can be predicted from records at an early age. The heritability (based on service-sire genetic component) of both total number of piglets born and survival to d 5 were 0.02, and the correlation between these effects and the additive genetic effect on boar taint ranged from 0.05 to -0

  9. Description of extended pre-harvest pig Salmonella surveillance-and-control programme and its estimated effect on food safety related to pork.

    PubMed

    Alban, L; Barfod, K; Petersen, J V; Dahl, J; Ajufo, J C; Sandø, G; Krog, H H; Aabo, S

    2010-11-01

    Salmonella in pork can be combated during pre- or post-harvest. For large slaughterhouses, post-harvest measures like decontamination might be cost-effective while this is less likely with small-to-medium sized slaughterhouses. In this study, pre-harvest measures might be more relevant. We describe an extended surveillance-and-control programme for Salmonella in finisher pigs, which, to establish equivalence to the Swedish control programme, is intended for implementation on the Danish island, Bornholm. The effect of the programme on food safety was estimated by analysing Salmonella data from pig carcasses originating from herds that would have qualified for the programme during 2006-2008. Food safety was interpreted as prevalence of Salmonella on carcasses as well as the estimated number of human cases of salmonellosis related to pork produced within the programme. Data from the Danish Salmonella programme were obtained from Bornholm. We used a simulation model developed to estimate the number of human cases based on the prevalence of Salmonella on carcass swabs. Herds are only accepted in the programme if they have one or less seropositive sample within the previous 6 months. In this way, the Salmonella load is kept to a minimum. The programme is not yet in operation and pigs that qualify for the programme are currently mixed at slaughter with those that do not qualify. Therefore, we had to assess the impact on the carcass prevalence indirectly. The prevalence of Salmonella in carcass swabs among qualifying herds was 0.46% for the 3 years as a whole, with 2006 as the year with highest prevalence. According to the simulation the expected number of human cases relating to pork produced within the programme was below 10. When the programme is in operation, an extra effect of separating pigs within the programme from those outside is expected to lower the prevalence of Salmonella even further.

  10. Longitudinal study of the persistence of antimicrobial-resistant campylobacter strains in distinct Swine production systems on farms, at slaughter, and in the environment.

    PubMed

    Quintana-Hayashi, Macarena P; Thakur, Siddhartha

    2012-04-01

    The objectives of this study were to compare and characterize the prevalence of antimicrobial-resistant (AR) Campylobacter in conventional and antimicrobial-free (ABF) production systems on farms, at slaughter, and in the environment. Fecal and environmental samples were collected from ABF farms (pigs, 1,239; environment, 797) and conventional farms (pigs, 1,650; environment, 1,325). At slaughter, we collected samples from carcasses, including postevisceration swabs, postchill swabs, and mesenteric lymph nodes from ABF systems (postevisceration swabs, 182; postchill swabs, 199; mesenteric lymph nodes, 184) and conventional systems (postevisceration swabs, 272; postchill swabs, 271; mesenteric lymph nodes, 255) at separate processing facilities. We also sampled the processing plant environment, including truck and lairage floor swab samples (ABF, 115; conventional, 90). Overall, a total of 2,908 Campylobacter isolates, including Campylobacter coli (farm, 2,557, 99.8%; slaughter, 341, 98.3%) and Campylobacter jejuni (farm, 4, 0.2%; slaughter, 6, 1.7%), were isolated in the study. There was no significant difference in the prevalence of Campylobacter between ABF and conventionally raised pigs (farrowing, P = 0.20; nursery, P = 0.06; finishing, P = 0.24) and the environment (P = 0.37). At slaughter, Campylobacter was isolated from all of the stages, including postchill. The highest frequencies of resistance were exhibited against tetracycline (ABF, 48.2%; conventional, 88.3%). Ciprofloxacin-resistant C. coli isolates were observed in conventionally raised (17.1%) and ABF (1.2%) pigs (P = 0.11). Antimicrobial use data from conventional farms indicated significant associations between oxytetracycline use and tetracycline resistance in the nursery pigs (P = 0.01), between tiamulin exposure and azithromycin and erythromycin resistance in nursery (P < 0.01) and finishing (P < 0.01) pigs, and between enrofloxacin exposure and ciprofloxacin and nalidixic acid resistance in

  11. Herd level husbandry factors associated with the serological Salmonella prevalence in finishing pig herds in The Netherlands.

    PubMed

    van der Wolf, P J; Wolbers, W B; Elbers, A R; van der Heijden, H M; Koppen, J M; Hunneman, W A; van Schie, F W; Tielen, M J

    2001-02-12

    A national program to reduce Salmonella in pork and pork products should include monitoring and intervention at farm level. To develop an adequate intervention strategy at farm level, risk factors for Salmonella infections in finishing pigs have to be determined. In this study, blood samples were collected randomly at two slaughterhouses from slaughter pigs. Samples were tested by the Dutch Salmonella ELISA, based on the O-antigens 1, 4, 5, 6, 7 and 12, using a cut-off of OD%=10. This ELISA has been calibrated against the Danish ELISA to give comparable results. Workers from herds from which at least forty blood samples had been collected, were asked to participate in a questionnaire. In total, 353 questionnaires were obtained and analysed. Significant risk factors associated with the proportion of seropositive samples were identified by multiple linear logistic regression. The feeding of a complete liquid feed containing fermented by-products and the omission of disinfection after pressure washing a compartment as part of an all-in/all-out procedure, were both associated with a lower Salmonella seroprevalence. A small to moderate herd size (<800 finishing pigs), a previous diagnosis of clinical Salmonella infection in the herd, the use of tylosin as an antimicrobial growth promoter in finishing feed, or herds which had more than 16% of the livers of their pigs condemned at the slaughterhouse as a result of white spots were associated with a higher Salmonella seroprevalence. Hypothetical intervention strategies based on these risk factors can be studied for their effect on the Salmonella seroprevalence and practical applicability in field studies.

  12. Isolation, antibiogram and pathogenicity of Salmonella spp. recovered from slaughtered food animals in Nagpur region of Central India

    PubMed Central

    Kalambhe, D. G.; Zade, N. N.; Chaudhari, S. P.; Shinde, S. V.; Khan, W.; Patil, A. R.

    2016-01-01

    Aim: To determine the prevalence, antibiogram and pathogenicity of Salmonella spp. in the common food animals slaughtered for consumption purpose at government approved slaughter houses located in and around Nagpur region during a period of 2010-2012. Materials and Methods: A total of 400 samples comprising 50 each of blood and meat from each slaughtered male cattle, buffaloes, pigs and goats were collected. Isolation was done by pre-enrichment in buffered peptone water and enrichment in Rappaport-Vassiliadis broth with subsequent selective plating onto xylose lysine deoxycholate agar. Presumptive Salmonella colonies were biochemically confirmed and analyzed for pathogenicity by hemolysin production and Congo red dye binding assay (CRDA). An antibiotic sensitivity test was performed to assess the antibiotic resistance pattern of the isolates. Results: A total of 10 isolates of Salmonella spp. from meat (3 from cattle, 1 from buffaloes and 6 from pigs) with an overall prevalence of 5% among food animals was recorded. No isolation was reported from any blood samples. Pathogenicity assays revealed 100% and 80% positivity for CRDA and hemolytic activity, respectively. Antimicrobial sensitivity test showed multi-drug resistance. The overall resistance of 50% was noted for trimethoprim followed by ampicillin (20%). A maximum sensitivity (80%) was reported to gentamycin followed by 40% each to ampicillin and trimethoprim, 30% to amikacin and 10% to kanamycin. Conclusion: The presence of multidrug resistant and potentially pathogenic Salmonella spp. in slaughtered food animals in Nagpur region can be a matter of concern for public health. PMID:27051204

  13. 77 FR 24873 - Modernization of Poultry Slaughter Inspection

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-26

    ... Modernization of Poultry Slaughter Inspection AGENCY: Food Safety and Inspection Service, USDA. ACTION: Proposed... the comment period for the proposed rulemaking ``Modernization of Poultry Slaughter Inspection'' and... advocacy organizations and two trade associations representing the poultry industry asked that FSIS...

  14. Pre-slaughter conditions, animal stress and welfare: current status and possible future research.

    PubMed

    Terlouw, E M C; Arnould, C; Auperin, B; Berri, C; Le Bihan-Duval, E; Deiss, V; Lefèvre, F; Lensink, B J; Mounier, L

    2008-10-01

    The present paper describes the main procedures used to slaughter fowl, pigs, calves and adult cattle, sheep, and farmed fish, starting on the farm and ending with the death of the animal at the abattoir. It reviews the currently known causes of stress, indicated by behavioural and physiological measurements on the animal level, and by post-mortem muscle metabolism. During the pre-slaughter period, psychological stress is due to changes of environment, social disturbances and handling, and physical stress is due to food deprivation, climatic conditions, fatigue, and sometimes pain. The exact causes of stress depend, however, on the characteristics of each species, including the rearing system. For fowl, bird catching and crating, duration and climatic conditions of transport and of lairage and shackling are the main known pre-slaughter stress factors. For pigs, stress is caused by fighting during mixing of pens, loading and unloading conditions, and introduction in the restrainer. Handling and novelty of the situation contribute to the stress reactions. For veal calves and adult cattle, disruption of the social group, handling, loading and sometimes unloading conditions, fatigue, novelty of the situation and for calves mixing with unfamiliar animals are known stress factors. Gathering and yarding of extensively reared lambs and sheep causes stress, particularly when shepherd dogs are used. Subsequent transport may induce fatigue, especially if sheep are commercialised through auctions or markets. In farmed fish, stress is predominantly related to environmental aspects such as temperature, oxygen, cleanliness of the water and, to a certain extent, stocking density and removal of the fish from the water. If transport and lairage conditions are good and their durations not too long, they may allow pigs, calves and adult cattle, sheep, and fish to rest. For certain species, it was shown that genetic origin and earlier experience influence reactions to the slaughter

  15. 9 CFR 149.6 - Slaughter facilities.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... slaughter facilities that are under continuous inspection by the Food Safety and Inspection Service or under State inspection that the Food Safety and Inspection Service has recognized as equivalent to Federal... Technology Programs, Technical Services Branch, 1400 Independence Ave., SW., Mail Stop 0272, Washington,...

  16. Auditing animal welfare at slaughter plants.

    PubMed

    Grandin, Temple

    2010-09-01

    The OIE Welfare Standards on slaughter transport, and killing of animals for disease control are basic minimum standards that every country should follow. The OIE, European Union, and many private standards used by commercial industry have an emphasis on animal based outcome standards instead of engineering based standards. Numerical scoring is used by both private industry and some governments to access animal welfare at slaughter plants. Five variables are measured. They are: 1) Percentage of animals effectively stunned on the first attempt, 2) Percentage rendered insensible, 3) Percentage that vocalize (bellow, moo, squeal) during handling and stunning, 4) Percentage that fall during handling, and 5) Percentage moved with an electric goad. Each one of these critical control points measures the outcome of many problems. A good animal welfare auditing system also has standards that prohibit really bad practices such as dragging, dropping, throwing, puntilla, and hoisting live animals before ritual slaughter. On farm and transport problems that can be measured at the slaughter plant are: percentage of lame animals, percentage of thin animals, percentage of dirty animals, percentage with sores, bruises or lesions, death losses, morbidity, and percentage of birds with broken wings and legs.

  17. 7 CFR 65.180 - Imported for immediate slaughter.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 3 2012-01-01 2012-01-01 false Imported for immediate slaughter. 65.180 Section 65... (CONTINUED) COUNTRY OF ORIGIN LABELING OF BEEF, PORK, LAMB, CHICKEN, GOAT MEAT, PERISHABLE AGRICULTURAL... immediate slaughter. Imported for immediate slaughter means imported into the United States for...

  18. 7 CFR 65.180 - Imported for immediate slaughter.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 3 2014-01-01 2014-01-01 false Imported for immediate slaughter. 65.180 Section 65... (CONTINUED) COUNTRY OF ORIGIN LABELING OF BEEF, PORK, LAMB, CHICKEN, GOAT MEAT, PERISHABLE AGRICULTURAL... immediate slaughter. Imported for immediate slaughter means imported into the United States for...

  19. 7 CFR 65.180 - Imported for immediate slaughter.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Imported for immediate slaughter. 65.180 Section 65... (CONTINUED) COUNTRY OF ORIGIN LABELING OF BEEF, PORK, LAMB, CHICKEN, GOAT MEAT, PERISHABLE AGRICULTURAL... immediate slaughter. Imported for immediate slaughter means imported into the United States for...

  20. 7 CFR 65.180 - Imported for immediate slaughter.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 3 2011-01-01 2011-01-01 false Imported for immediate slaughter. 65.180 Section 65... (CONTINUED) COUNTRY OF ORIGIN LABELING OF BEEF, PORK, LAMB, CHICKEN, GOAT MEAT, PERISHABLE AGRICULTURAL... immediate slaughter. Imported for immediate slaughter means imported into the United States for...

  1. 7 CFR 65.180 - Imported for immediate slaughter.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 3 2013-01-01 2013-01-01 false Imported for immediate slaughter. 65.180 Section 65... (CONTINUED) COUNTRY OF ORIGIN LABELING OF BEEF, PORK, LAMB, CHICKEN, GOAT MEAT, PERISHABLE AGRICULTURAL... immediate slaughter. Imported for immediate slaughter means imported into the United States for...

  2. Usefulness of food chain information provided by Dutch finishing pig producers to control antibiotic residues in pork.

    PubMed

    van Wagenberg, Coen P A; Backus, Gé B C; van der Vorst, Jack G A J; Urlings, Bert A P

    2012-11-01

    The EU prescribes that food business operators must use food chain information to assist in food safety control. This study analyses usefulness of food chain information about antibiotic usage covering the 60-day period prior to delivery of pigs to slaughter in the control of antibiotic residues in pork. A dataset with 479 test results for antibiotic residues in tissue samples of finishing pigs delivered to a Dutch slaughter company was linked to information provided by pig producers about antibiotic usage in these finishing pigs. Results show that twice as many producers reported using antibiotics in the group of 82 producers with antibiotic residues (11.0%) compared to the group without antibiotic residues (5.5%) (p=0.0686). For 89% of consignments with a finishing pig with antibiotic residues, the producer reported 'did not use antibiotics'. Food chain information about antibiotic usage provided by Dutch pig producers was no guarantee for absence of antibiotic residues in delivered finishing pigs.

  3. Making slaughterhouses more humane for cattle, pigs, and sheep.

    PubMed

    Grandin, Temple

    2013-01-01

    When a stunning method is being evaluated, it is essential that the animal-handling and restraint methods that are used with it are also examined. This makes it possible to determine the effect of the entire system on animal welfare. Cattle, pigs, and sheep will move easily through the races at a slaughter plant if visual distractions such as reflections on shiny metal, dangling chains, moving equipment, or people up ahead are removed. The most important scientific research on captive bolt, CO2, and electrical stunning methods is reviewed. A common mistake made by people evaluating insensibility is to misinterpret reflexive leg kicks as a sign of return to sensibility. When religious slaughter is being evaluated, the variable of how the animal is restrained must be separated from the variable of slaughter without stunning. Slaughter can be done with a high level of animal welfare.

  4. Cardiac ganglioneuroma in a juvenile pig

    PubMed Central

    INOUE, Ryoko; JOMA, Ikumi; OTSUBO, Koji; MATSUTAKE, Hiroshi; YANAI, Tokuma; SAKAI, Hiroki

    2015-01-01

    A cardiac mass (3 × 5 × 3 cm) was detected at the base between the right auricular wall and right vena cava of a slaughtered 6-month-old female mixed-breed pig during a meat inspection. The tumor comprised infiltrative prominent interweaving fascicles of Schwann cells with Verocay bodies. Moreover, the ganglion cells were scattered or aggregated throughout the neoplastic tissue. The ganglion and Schwann cells had neither cellular atypism nor mitosis. On the basis of the bearing site as well as the morphological and immunohistochemical features, this is the first case of a cardiac ganglioneuroma in a pig. PMID:26256406

  5. Trend Analyses of Nitrate in Danish Groundwater

    NASA Astrophysics Data System (ADS)

    Hansen, B.; Thorling, L.; Dalgaard, T.; Erlandsen, M.

    2012-04-01

    This presentation assesses the long-term development in the oxic groundwater nitrate concentration and nitrogen (N) loss due to intensive farming in Denmark. Firstly, up to 20-year time-series from the national groundwater monitoring network enable a statistically systematic analysis of distribution, trends and trend reversals in the groundwater nitrate concentration. Secondly, knowledge about the N surplus in Danish agriculture since 1950 is used as an indicator of the potential loss of N. Thirdly, groundwater recharge CFC (Chlorofluorocarbon) age determination allows linking of the first two dataset. The development in the nitrate concentration of oxic groundwater clearly mirrors the development in the national agricultural N surplus, and a corresponding trend reversal is found in groundwater. Regulation and technical improvements in the intensive farming in Denmark have succeeded in decreasing the N surplus by 40% since the mid 1980s while at the same time maintaining crop yields and increasing the animal production of especially pigs. Trend analyses prove that the youngest (0-15 years old) oxic groundwater shows more pronounced significant downward nitrate trends (44%) than the oldest (25-50 years old) oxic groundwater (9%). This amounts to clear evidence of the effect of reduced nitrate leaching on groundwater nitrate concentrations in Denmark. Are the Danish groundwater monitoring strategy obtimal for detection of nitrate trends? Will the nitrate concentrations in Danish groundwater continue to decrease or are the Danish nitrate concentration levels now appropriate according to the Water Framework Directive?

  6. Impact of test sensitivity and specificity on pig producer incentives to control Mycobacterium avium infections in finishing pigs.

    PubMed

    van Wagenberg, Coen P A; Backus, Gé B C; Wisselink, Henk J; van der Vorst, Jack G A J; Urlings, Bert A P

    2013-09-01

    In this paper we analyze the impact of the sensitivity and specificity of a Mycobacterium avium (Ma) test on pig producer incentives to control Ma in finishing pigs. A possible Ma control system which includes a serodiagnostic test and a penalty on finishing pigs in herds detected with Ma infection was modelled. Using a dynamic optimization model and a grid search of deliveries of herds from pig producers to slaughterhouse, optimal control measures for pig producers and optimal penalty values for deliveries with increased Ma risk were identified for different sensitivity and specificity values. Results showed that higher sensitivity and lower specificity induced use of more intense control measures and resulted in higher pig producer costs and lower Ma seroprevalence. The minimal penalty value needed to comply with a threshold for Ma seroprevalence in finishing pigs at slaughter was lower at higher sensitivity and lower specificity. With imperfect specificity a larger sample size decreased pig producer incentives to control Ma seroprevalence, because the higher number of false positives resulted in an increased probability of rejecting a batch of finishing pigs irrespective of whether the pig producer applied control measures. We conclude that test sensitivity and specificity must be considered in incentive system design to induce pig producers to control Ma in finishing pigs with minimum negative effects.

  7. A description of smallholder pig production systems in eastern Indonesia.

    PubMed

    Leslie, Edwina E C; Geong, Maria; Abdurrahman, Muktasam; Ward, Michael P; Toribio, Jenny-Ann L M L

    2015-03-01

    Pig farming is a common practice among smallholder farmers in Nusa Tenggara Timur province (NTT), eastern Indonesia. To understand their production systems a survey of smallholder pig farmers was conducted. Eighteen villages were randomly selected across West Timor, Flores and Sumba islands, and 289 pig farmers were interviewed. Information on pig management, biosecurity practices, pig movements and knowledge of pig health and disease, specifically classical swine fever was collected. The mean number of pigs per herd was 5.0 (not including piglets), and total marketable herd size (pigs≥two months of age) did not differ significantly between islands (P=0.215). Chickens (71%) and dogs (62%) were the most commonly kept animal species in addition to pigs. Pigs were mainly kept as a secondary income source (69%) and 83% of farmers owned at least one sow. Seventy-four percent (74%) of pigs were housed in a kandang (small bamboo pen) and 25% were tethered. Pig feeds were primarily locally sourced agricultural products (93%). The majority of farmers had no knowledge of classical swine fever (91%) and biosecurity practices were minimal. Forty-five percent (45%) reported to consuming a pig when it died and 74% failed to report cases of sick or dead pigs to appropriate authorities. Sixty-five percent (65%) of farmers reported that a veterinarian or animal health worker had never visited their village. Backyard slaughter was common practice (55%), with meat mainly used for home consumption (89%). Most (73%) farmers purchased pigs in order to raise the animal on their farm with 36% purchasing at least one pig within the last year. Predominantly fattener pigs (34%) were given as gifts for celebratory events, most commonly for funerals (32%), traditional ceremonies (27%) and marriages (10%). For improved productivity of this traditional low-input system, research incorporating farming training and improved knowledge on pig disease and biosecurity needs to be integrated with

  8. Effect of sex and time to slaughter (transportation and lairage duration) on the levels of cortisol, creatine kinase and subsequent relationship with pork quality.

    PubMed

    Jama, N; Maphosa, V; Hoffman, L C; Muchenje, V

    2016-06-01

    The study determined the effect of sex and time to slaughter on cortisol and creatine kinase levels, and pork quality in commercial crossbred pigs. Saliva samples were before collected transportation, on arrival at the abattoir, and after a 20 hour lairage period. Cortisol levels from saliva (SC), serum (SeC) and urine (UC) were determined. Creatine kinase (CK) levels were determined from serum samples. Fifteen boars vs. 15 gilts were immediately slaughtered on arrival (SOA), and the other 15 boars vs. 15 gilts were rested for 20 h before slaughter. Meat quality parameters were also determined. In both sexes, SC significantly increased in response to time to slaughter. There was a significant interaction of sex and time to slaughter on SeC. Gilts had higher CK levels and lower muscle L* values than boars. There were correlations among baseline SC, SeC, UC and most meat quality parameters. Time to slaughter influenced levels of SC, UC, CK and pork quality between boars and gilts.

  9. Chronological study of Mycoplasma hyopneumoniae infection, seroconversion and associated lung lesions in vaccinated and non-vaccinated pigs.

    PubMed

    Sibila, M; Nofrarías, M; López-Soria, S; Segalés, J; Valero, O; Espinal, A; Calsamiglia, M

    2007-05-16

    A field trial was conducted to study Mycoplasma hyopneumoniae (Mh) infection dynamics by nested polymerase chain reaction (nPCR) and serology in pigs of a farm affected by enzootic pneumonia (EP). Moreover, correlation of Mh detection at different respiratory tract sites with presence of EP gross and microscopic lung lesions was assessed. These parameters were studied and compared between vaccinated (two doses at 1 and 3 weeks of age versus one dose at 6 weeks of age) and non-vaccinated pigs. Animals were monitored from birth to slaughter by nPCR from nasal swabs and by serology. From 3 to 22 weeks of age, an average of three pigs per treatment and per batch were necropsied (n = 302). The remaining pigs were sent to the slaughter (n = 103). Nasal, bronchial and tonsillar swabs were taken from the necropsied/slaughtered pigs; gross and microscopic EP-suggestive lung lesions were also assessed. Single and double vaccination resulted in earlier seroconversion and higher percentage of Mh seropositive pigs compared to control group. At slaughter, double vaccinated pigs showed lower percentage of EP-compatible gross lung lesions and lower Mh prevalence at upper respiratory tract sites (nasal cavity and tonsil) than control pigs.

  10. Dehydration indicators for broiler chickens at slaughter.

    PubMed

    Vanderhasselt, R F; Buijs, S; Sprenger, M; Goethals, K; Willemsen, H; Duchateau, L; Tuyttens, F A M

    2013-03-01

    Freedom of (prolonged) thirst is considered to be of paramount importance for animal welfare. This emotion normally results from dehydration, which can be measured using physiological indicators. Because no reliable physiological indicator for thirst was available for broilers, we aimed to identify such a measure in this study. This indicator would ideally be integrated into quality control systems in commercial slaughter plants. In the first experiment, water deprivation was manipulated systematically by withdrawing water for different durations (total water withdrawal for 0 (control), 24, 36, or 48 h, or a 10-d period with restricted access to water for 2 times 10 min per day). A significant decrease in drained blood content and BW occurred from 36 h of total water deprivation onward (both P = 0.03), whereas long-term restricted access tended to decrease drained blood content (P = 0.05). No effect of water deprivation or restriction on skin turgor was found. In the second experiment, water was withdrawn for 0 (control), 6, 12, 24, or 48 h. Plasma chloride concentration was increased after 6 h of water withdrawal, but did not rise further with longer withdrawal. If assessed at slaughter, chloride will thus mainly reflect the catching-to-slaughter interval. In contrast, plasma creatinine and hematocrit levels showed a numerical decrease after 6 h of water withdrawal, but rose again after prolonged withdrawal. Plasma creatinine values were significantly higher in 24-h-deprived birds than in 6-h-deprived birds (P < 0.01), allowing for discernment between water withdrawal during catching and transport from dehydration that had occurred on the farm. Blood sodium concentrations and plasma osmolality showed a steady increment between 0 and 24 h of water deprivation (P < 0.001 and P < 0.001 for both), and may thus be used to assess the combined effects of water deprivation on farm and during the catching-to-slaughter interval. These findings may form the basis of an on

  11. Forensic cases of bruises in pigs.

    PubMed

    Barington, K; Jensen, H E

    2013-11-30

    Bruises in pigs inflicted by blunt trauma are a significant animal welfare problem, and affected skin and underlying muscle are regularly submitted for forensic investigation. Central to the evaluation is an assessment of the age of the bruises. This paper presents cases of bruises in pigs sent for forensic investigation that were collected retrospectively. Data comprised photographs of the gross lesions, slides for histology, and written reports. The time from collecting the animals at the farms and delivery to the slaughterhouse was recorded together with the time of slaughter. Since 2005 there has been an increase in cases, with a peak in 2008 and 2009 of 40 cases for each year. At gross examination, the pattern of bruises often reflected the type of object which caused them. Histologically, haemorrhage and cellular infiltrations were frequently present. Currently, the age of bruises may be estimated to be more or less than four hours based on a porcine bruise model. In bruises more than four hours old, estimations of two-hour intervals are used based on studies of wound healing. The time from collecting the pigs at the farms until slaughter was between one and four hours in 44.1 per cent of cases, during which time the pigs had been handled by several people. In addition, in 22.0 per cent of cases of bruising an inflammatory response was absent, making it impossible to estimate the age of the bruise.

  12. [The development of antibiotics use in Danish food production].

    PubMed

    Jensen, Vibeke Frøkjær

    2011-11-07

    In the Danish husbandry antimicrobial growth promoters were phased out 1994-2000 and the therapeutic use has been increasingly regulated. Hitherto, a minimum in therapeutic use was reached in 1997. The antimicrobial use in pigs increased by 44% during 2002-2009; a 12% decrease in second half of 2010 was likely due to the announcement of the "yellow card" regulation. From July 2010, a voluntary two years stop of cephalosporins use in pigs was realized, due to increasing occurrence of extended spectrum beta lactamase (ESBL) resistance in animal and meat isolates; highest levels of ESBL are observed in imported poultry.

  13. Shechita (Kosher slaughtering) and European legislation.

    PubMed

    Pozzi, Paolo S; Waner, Trevor

    2017-03-31

    Shechita is the procedure of killing or slaughtering animals for food production, according to Jewish tradition and it is performed without prior stunning. USA and European legislations conditionally allow slaughter without prior stunning in the frame of religion freedom (USA) or religious/cultural traditions (EU); nevertheless some traditional events in Europe de nitely represent a concern for animal welfare. It is possible to identify animal welfare issues in the rules for shechita: correct restrain of the animal; adequacy of the instrument (knife); technical ability of the operator. Animals restrain techniques evolved along the time in order to accomplish to less stressful immobilization of animals in course of shechita. When performed in the right way, shechita cannot be framed as negligent or intentionally painful, distressing or inducing su ering to animals. Today's stunning techniques raise concerns relative to adequacy and e ectiveness of stunning on animals, with welfare implications due to automatism of next dressing procedures. Shechita needs in Europe are in line with average meat demand by non Jewish population.

  14. Identification and characterization of pigs prone to producing 'RSE' (reddish-pink, soft and exudative) meat in normal pigs.

    PubMed

    Cheah, K S; Cheah, A M; Just, A

    1998-03-01

    RSE (reddish-pink, soft and exudative) meat was investigated using pigs of three different halothane genotypes. A significantly lower pH(1h), value was observed in RSE compared with that of RFN (red, firm and non-exudative) -meat, both of which have values higher than 6.0 at 1 hr post-mortem. Drip loss (%) in RSE-meat was ≥7%, which was twice that of RFN-meat. Normal values for fibre optic probe and Minolta L and a were observed for RSE-meat. RSE-meat could be derived from NN and Nn pigs, and its formation could be induced from RFN-prone pigs by poor post-slaughter management. Pigs expected to produce RSE-meat were identified using small biopsy samples of M. longissimus dorsi (LD). Predicted RSE-meat in live pigs was confirmed by post-mortem assessments of meat quality using LD muscle. With NN Landrace-Yorkshire × Duroc pigs, 15.6% were identified to be RSE-prone in live pigs, and a further 6.7% RSE was induced after slaughter from RFN pigs. The rate of glycolysis determined from biopsy LD samples and at 1 hr post-mortem (pH(1h)) were significantly (p < 0.001) faster in RSE than in RFN-prone pigs, but significantly slower than those of PSE-prone pigs. Good correlations (p < 0.001) were observed between biopsy fluid (F) values, an indicator of water-holding capacity (WHC), and drip loss (r = 0.652) from post-mortem LD muscle, and between biopsy pH (F), an indicator for the rate of glycolysis, and F (r = -0.828). These results show that the skeletal muscle test using biopsy LD muscle could be employed to reduce the incidence of RSE-meat.

  15. Effects of marketing group on the variability of fresh loin, belly, and fresh and processed ham quality from pigs sourced from a commercial processing facility

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The objective was to quantify the effect of marketing group (MG) on the variability of primal quality. Pigs (N=7,684) were slaughtered in 3 MGs from 8 barns. Pigs were from genetic selection programs focused on lean growth (L; group 1 n=1,131; group 2 n=1,466; group 3 n=1,030) or superior meat qua...

  16. 9 CFR 146.8 - Terminology and classification; slaughter plants.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Terminology and classification; slaughter plants. 146.8 Section 146.8 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE... General Provisions § 146.8 Terminology and classification; slaughter plants. Participating...

  17. 9 CFR 146.8 - Terminology and classification; slaughter plants.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Terminology and classification; slaughter plants. 146.8 Section 146.8 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE... General Provisions § 146.8 Terminology and classification; slaughter plants. Participating...

  18. 9 CFR 146.8 - Terminology and classification; slaughter plants.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Terminology and classification; slaughter plants. 146.8 Section 146.8 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE... General Provisions § 146.8 Terminology and classification; slaughter plants. Participating...

  19. 9 CFR 309.12 - Emergency slaughter; inspection prior to.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 2 2014-01-01 2014-01-01 false Emergency slaughter; inspection prior to. 309.12 Section 309.12 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT..., the animals shall be inspected immediately before slaughter, whether theretofore inspected or...

  20. 9 CFR 309.12 - Emergency slaughter; inspection prior to.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 2 2011-01-01 2011-01-01 false Emergency slaughter; inspection prior to. 309.12 Section 309.12 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT..., the animals shall be inspected immediately before slaughter, whether theretofore inspected or...

  1. 9 CFR 309.12 - Emergency slaughter; inspection prior to.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 2 2012-01-01 2012-01-01 false Emergency slaughter; inspection prior to. 309.12 Section 309.12 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT..., the animals shall be inspected immediately before slaughter, whether theretofore inspected or...

  2. 9 CFR 146.8 - Terminology and classification; slaughter plants.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Terminology and classification; slaughter plants. 146.8 Section 146.8 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE... General Provisions § 146.8 Terminology and classification; slaughter plants. Participating...

  3. 9 CFR 309.12 - Emergency slaughter; inspection prior to.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Emergency slaughter; inspection prior to. 309.12 Section 309.12 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT..., the animals shall be inspected immediately before slaughter, whether theretofore inspected or...

  4. 9 CFR 146.8 - Terminology and classification; slaughter plants.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Terminology and classification; slaughter plants. 146.8 Section 146.8 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE... General Provisions § 146.8 Terminology and classification; slaughter plants. Participating...

  5. Building the Engaged Campus: An Interview with John Brooks Slaughter.

    ERIC Educational Resources Information Center

    Maeda, Donna

    1999-01-01

    Interview with John Brooks Slaughter, recent president of Occidental College, focuses on his leadership during a period when the college dramatically increased its minority enrollment, recruited an impressively diverse faculty, and launched various community outreach programs. Explores Slaughter's views concerning diversity as part of a college's…

  6. 5. View of interior, EPA Farm Lab Building 1506 slaughter ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. View of interior, EPA Farm Lab Building 15-06 slaughter addition (featuring cold slaughter area), facing north-northeast - Nevada Test Site, Environmental Protection Agency Farm, Laboratory Building, Area 15, Yucca Flat, 10-2 Road near Circle Road, Mercury, Nye County, NV

  7. 77 FR 4407 - Modernization of Poultry Slaughter Inspection

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-27

    ...The Food Safety and Inspection Service (FSIS) is proposing a new inspection system for young chicken and turkey slaughter establishments that would replace the current Streamlined Inspection System (SIS), the New Line Speed Inspection System (NELS), and the New Turkey Inspection System (NTIS). The Agency is also proposing several changes that would affect all establishments that slaughter......

  8. 9 CFR 93.326 - Horses for immediate slaughter.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Horses for immediate slaughter. 93.326... FOR MEANS OF CONVEYANCE AND SHIPPING CONTAINERS Horses Mexico 18 § 93.326 Horses for immediate slaughter. Horses may be imported from Mexico, subject to the applicable provisions of §§ 93.321,...

  9. 9 CFR 93.326 - Horses for immediate slaughter.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Horses for immediate slaughter. 93.326... FOR MEANS OF CONVEYANCE AND SHIPPING CONTAINERS Horses Mexico 18 § 93.326 Horses for immediate slaughter. Horses may be imported from Mexico, subject to the applicable provisions of §§ 93.321,...

  10. 9 CFR 93.326 - Horses for immediate slaughter.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Horses for immediate slaughter. 93.326... FOR MEANS OF CONVEYANCE AND SHIPPING CONTAINERS Horses Mexico 18 § 93.326 Horses for immediate slaughter. Horses may be imported from Mexico, subject to the applicable provisions of §§ 93.321,...

  11. 9 CFR 93.326 - Horses for immediate slaughter.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Horses for immediate slaughter. 93.326... FOR MEANS OF CONVEYANCE AND SHIPPING CONTAINERS Horses Mexico 18 § 93.326 Horses for immediate slaughter. Horses may be imported from Mexico, subject to the applicable provisions of §§ 93.321,...

  12. 9 CFR 93.429 - Ruminants for immediate slaughter.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Ruminants for immediate slaughter. 93...; REQUIREMENTS FOR MEANS OF CONVEYANCE AND SHIPPING CONTAINERS Ruminants Mexico 10 § 93.429 Ruminants for immediate slaughter. Ruminants, other than sheep and goats, may be imported from Mexico, subject to...

  13. 9 CFR 93.429 - Ruminants for immediate slaughter.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Ruminants for immediate slaughter. 93...; REQUIREMENTS FOR MEANS OF CONVEYANCE AND SHIPPING CONTAINERS Ruminants Mexico 10 § 93.429 Ruminants for immediate slaughter. Ruminants, other than sheep and goats, may be imported from Mexico, subject to...

  14. 9 CFR 93.429 - Ruminants for immediate slaughter.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Ruminants for immediate slaughter. 93...; REQUIREMENTS FOR MEANS OF CONVEYANCE AND SHIPPING CONTAINERS Ruminants Mexico 10 § 93.429 Ruminants for immediate slaughter. Ruminants, other than sheep and goats, may be imported from Mexico, subject to...

  15. 9 CFR 93.429 - Ruminants for immediate slaughter.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Ruminants for immediate slaughter. 93...; REQUIREMENTS FOR MEANS OF CONVEYANCE AND SHIPPING CONTAINERS Ruminants Mexico 10 § 93.429 Ruminants for immediate slaughter. Ruminants, other than sheep and goats, may be imported from Mexico, subject to...

  16. 9 CFR 93.429 - Ruminants for immediate slaughter.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Ruminants for immediate slaughter. 93...; REQUIREMENTS FOR MEANS OF CONVEYANCE AND SHIPPING CONTAINERS Ruminants Mexico 10 § 93.429 Ruminants for immediate slaughter. Ruminants, other than sheep and goats, may be imported from Mexico, subject to...

  17. Effects of slaughter age and mass selection on slaughter and carcass characteristics in 2 lines of Japanese quail.

    PubMed

    Narinc, Dogan; Karaman, Emre; Aksoy, Tulin

    2014-03-01

    The aim of this study was to evaluate the relationship between slaughter age and slaughter-carcass characteristics in 2 quail lines. With this aim, a Japanese quail flock subjected to mass selection to increase BW for 4 generations and a control flock that randomly mated for 4 generations were used. Birds of both lines were slaughtered at 4, 5, 6, 7, and 8 wk of age. Weights of carcass, breast, leg, wing, edible inner organs, and abdominal fat, and their percentages in BW were measured. Short-term mass selection for increased BW resulted in an increase for all slaughter and carcass traits, except edible inner organ percentage. Slaughter age had a significant effect on the studied traits, indicating that the BW and weight of carcass, carcass parts, abdominal fat, edible inner organs, and percentage of abdominal fat increased with increased slaughter age. Conversely, the carcass yield and percentages of carcass parts and edible inner organs were decreased with an increase in slaughter age. The present study showed that deterioration in carcass quality occurred with an increase in slaughter age. Furthermore, the differences between the carcass weights over the different ages ranged between 16.83 to 22.45% in favor of the selection line after a short-term mass selection.

  18. Halal and kosher slaughter methods and meat quality: a review.

    PubMed

    Farouk, M M; Al-Mazeedi, H M; Sabow, A B; Bekhit, A E D; Adeyemi, K D; Sazili, A Q; Ghani, A

    2014-11-01

    There are many slaughter procedures that religions and cultures use around the world. The two that are commercially relevant are the halal and kosher methods practiced by Muslims and Jews respectively. The global trade in red meat and poultry produced using these two methods is substantial, thus the importance of the quality of the meat produced using the methods. Halal and kosher slaughter per se should not affect meat quality more than their industrial equivalents, however, some of their associated pre- and post-slaughter processes do. For instance, the slow decline in blood pressure following a halal pre-slaughter head-only stun and neck cut causes blood splash (ecchymosis) in a range of muscles and organs of slaughtered livestock. Other quality concerns include bruising, hemorrhages, skin discoloration and broken bones particularly in poultry. In addition to these conventional quality issues, the "spiritual quality" of the meat can also be affected when the halal and kosher religious requirements are not fully met during the slaughter process. The nature, causes, importance and mitigations of these and other quality issues related to halal and kosher slaughtering and meat production using these methods are the subjects of this review.

  19. Religious slaughter: evaluation of current practices in selected countries.

    PubMed

    Velarde, A; Rodriguez, P; Dalmau, A; Fuentes, C; Llonch, P; von Holleben, K V; Anil, M H; Lambooij, J B; Pleiter, H; Yesildere, T; Cenci-Goga, B T

    2014-01-01

    As part of the project "Religious slaughter (DIALREL): improving knowledge and expertise through dialogue and debate on issues of welfare, legislation and socio-economic aspects", this paper discusses an evaluation of current practices during Halal and Shechita slaughter in cattle, sheep, goats and poultry. During religious slaughter, animals are killed with and without stunning by a transverse incision across the neck that is cutting the skin, muscles (brachiocephalic, sternocephalic, sternohyoid, and sternothyroid), trachea, esophagus, carotid arteries, jugular veins and the major, superficial and deep nerves of the cervical plexus. In this report, the restraint methods, stunning, neck cutting, exsanguination, slaughter techniques and postcut handling in the abattoir were assessed for religious slaughter. Information about the procedures used during religious slaughter in Belgium, Germany, Italy, the Netherlands, Spain, the UK, Turkey and Australia was collected by means of spot visits to abattoirs. To standardize the information gathered during the spot visits three guidelines were designed, one for each species, and translated into the national languages of the countries involved. The document included questions on the handling and restraint methods (stunning, neck cutting/exsanguination/slaughter techniques and postcut handling performed under religious practices) and for pain and distress of the animal during the restraint, neck cutting and induction to death in each abattoir. Results showed differences in the time from restraining to stun and to cut in the neck cutting procedures and in the time from cut to death.

  20. Danish Gynecological Cancer Database

    PubMed Central

    Sørensen, Sarah Mejer; Bjørn, Signe Frahm; Jochumsen, Kirsten Marie; Jensen, Pernille Tine; Thranov, Ingrid Regitze; Hare-Bruun, Helle; Seibæk, Lene; Høgdall, Claus

    2016-01-01

    Aim of database The Danish Gynecological Cancer Database (DGCD) is a nationwide clinical cancer database and its aim is to monitor the treatment quality of Danish gynecological cancer patients, and to generate data for scientific purposes. DGCD also records detailed data on the diagnostic measures for gynecological cancer. Study population DGCD was initiated January 1, 2005, and includes all patients treated at Danish hospitals for cancer of the ovaries, peritoneum, fallopian tubes, cervix, vulva, vagina, and uterus, including rare histological types. Main variables DGCD data are organized within separate data forms as follows: clinical data, surgery, pathology, pre- and postoperative care, complications, follow-up visits, and final quality check. DGCD is linked with additional data from the Danish “Pathology Registry”, the “National Patient Registry”, and the “Cause of Death Registry” using the unique Danish personal identification number (CPR number). Descriptive data Data from DGCD and registers are available online in the Statistical Analysis Software portal. The DGCD forms cover almost all possible clinical variables used to describe gynecological cancer courses. The only limitation is the registration of oncological treatment data, which is incomplete for a large number of patients. Conclusion The very complete collection of available data from more registries form one of the unique strengths of DGCD compared to many other clinical databases, and provides unique possibilities for validation and completeness of data. The success of the DGCD is illustrated through annual reports, high coverage, and several peer-reviewed DGCD-based publications. PMID:27822089

  1. Presence of hepatitis E virus in a naturally infected swine herd from nursery to slaughter.

    PubMed

    Leblanc, Danielle; Ward, Pierre; Gagné, Marie-Josée; Poitras, Elyse; Müller, Peter; Trottier, Yvon-Louis; Simard, Carole; Houde, Alain

    2007-06-30

    Hepatitis E virus has recently been recognized as having zoonotic potential and could be transmitted from pig to human. Pigs are identified as a potential animal reservoir and HEV is highly prevalent in the swine population around the world. In this study, the presence of HEV was investigated in 51 subjects reared on a simulated commercial farm setting from the age of 2 weeks up to slaughter. Samples were collected on four occasions: at 2, 8, and 18 weeks and between 22-29 weeks of age. Anti-HEV IgG in plasma samples, presence of HEV RNA in plasma samples and feces were monitored. At 2 weeks of age, HEV RNA was detected in feces of 6 subjects (11.8%) but not in their plasma. At 8 weeks, HEV was detected in feces of 27 subjects (52.9%) and in plasma of one subject. At 18 weeks, HEV was detected in feces of 44 subjects (86.2%) and in plasma of 24 subjects (47.1%). At slaughter time (22-29 weeks), HEV was present in plasma of 6 subjects (11.8%) and in stools of 21 subjects (41.2%). Spread of the virus inside the population was evaluated by comparison of means (paired t-test, P<0.05) of anti-HEV IgG ELISA results from the 4 bleedings. Significant differences were noted between the results of populations at 8 and 18 weeks and also between 18 and 22 to 29 weeks indicating an immune response to the virus. Based on the comparison of a 304 nucleotides sequence of the 5' ORF 2 gene, all amplified fragments clustered in genotype 3a.

  2. Seroprevalence of hepatitis E virus in pigs from different farming systems in The Netherlands.

    PubMed

    Rutjes, S A; Bouwknegt, M; van der Giessen, J W; de Roda Husman, A M; Reusken, C B E M

    2014-04-01

    Sporadic nontravel-related hepatitis E virus (HEV) infections have been reported in industrialized countries. These infections are caused by zoonotic HEV genotypes 3 and 4 that circulate in swine, wild boar, and deer. In The Netherlands, HEV RNA has been detected in >50% of the pig farms, and HEV-specific antibodies were detected in ∼70% of the slaughter pigs. In the current study, HEV seroprevalences were investigated in pigs raised on conventional, free-range, and organic farms in The Netherlands. Differences in seroprevalence may indicate different exposure routes or transmission dynamics within pig herds for HEV. In 2004, serum samples of 846 fattening pigs were obtained from farms that applied conventional (265 pigs at 24 farms), organic (417 pigs at 42 farms), and free-range (164 pigs at 12 farms) farming. HEV-specific antibodies were detected in samples from all conventional and free-range pig farms and in 41 of 42 organic pig farms, indicating that the probability of introducing HEV on a farm appeared to be equal for the different farming types. The estimated average within-herd seroprevalence was significantly higher for pigs raised on organic farms (89%) than for pigs raised on conventional farms (72%, P = 0.04) and nearly significant for pigs raised on free-range farms (76%, P = 0.06). Six of ten organic farms were estimated to have a withinherd seroprevalence of >95%, compared with 1 of 10 and 4 of 10 of the free-range and conventional pig farms, respectively. This suggests a higher force of infection with HEV for pigs reared on organic farms compared with pigs reared on conventional or free-range farms. This may be due to repetitive exposure to HEV caused by farming system-specific housing conditions, such as a greater contact frequency between pigs and more exposure to pig manure, increasing the transmission rate.

  3. The Danish Melanoma Database

    PubMed Central

    Hölmich, Lisbet Rosenkrantz; Klausen, Siri; Spaun, Eva; Schmidt, Grethe; Gad, Dorte; Svane, Inge Marie; Schmidt, Henrik; Lorentzen, Henrik Frank; Ibfelt, Else Helene

    2016-01-01

    Aim of database The aim of the database is to monitor and improve the treatment and survival of melanoma patients. Study population All Danish patients with cutaneous melanoma and in situ melanomas must be registered in the Danish Melanoma Database (DMD). In 2014, 2,525 patients with invasive melanoma and 780 with in situ tumors were registered. The coverage is currently 93% compared with the Danish Pathology Register. Main variables The main variables include demographic, clinical, and pathological characteristics, including Breslow’s tumor thickness, ± ulceration, mitoses, and tumor–node–metastasis stage. Information about the date of diagnosis, treatment, type of surgery, including safety margins, results of lymphoscintigraphy in patients for whom this was indicated (tumors > T1a), results of sentinel node biopsy, pathological evaluation hereof, and follow-up information, including recurrence, nature, and treatment hereof is registered. In case of death, the cause and date are included. Currently, all data are entered manually; however, data catchment from the existing registries is planned to be included shortly. Descriptive data The DMD is an old research database, but new as a clinical quality register. The coverage is high, and the performance in the five Danish regions is quite similar due to strong adherence to guidelines provided by the Danish Melanoma Group. The list of monitored indicators is constantly expanding, and annual quality reports are issued. Several important scientific studies are based on DMD data. Conclusion DMD holds unique detailed information about tumor characteristics, the surgical treatment, and follow-up of Danish melanoma patients. Registration and monitoring is currently expanding to encompass even more clinical parameters to benefit both patient treatment and research. PMID:27822097

  4. More Likely we would BE Rituvally Slaughtered

    NASA Astrophysics Data System (ADS)

    Sloot, Peter M. A.

    Astronomers of the Maya and babylonian civilizations were brilliant in predicting astronomical events. For instance, from meticulous observations of the sun, Moon, Venus, and Jupiter they were able to predict with astonishing accuracy the 584-day cycle of Venus or the details of the celestial track of Jupiter. Yet they had no clue about our heliocentric solar system, they believed that the earth was flat, and they were completely ignorant of the real movement of stars and planets while being convinced that the sky was supported by four jaguars, each holding up a corner of the sky. if we would be sent back in time and speak to them about the planets orbiting the sun, they would laugh at us and challenge us to come with the accurate predictions that they were able to make. With all our knowledge, but without thousands of years of technological development, we would not be able to come close to any of their predictions. so being laughed at would be a small punishment, more likely we would be ritually slaughtered...

  5. Transportation of market-weight pigs: II. effect of season and location within truck on behavior with an eight-hour transport.

    PubMed

    Torrey, S; Bergeron, R; Faucitano, L; Widowski, T; Lewis, N; Crowe, T; Correa, J A; Brown, J; Hayne, S; Gonyou, H W

    2013-06-01

    Transportation of pigs to slaughter has the potential to negatively impact animal welfare, particularly in hot temperatures and over long transport durations. The objective of this experiment was to determine if season and location within vehicle influenced the behavior of market-weight pigs during loading, transit, unloading, and lairage after a long-distance trip to slaughter. On a pot-belly truck, 1,170 pigs were transported (n = 195 pigs/wk in 7 experimental compartments) for 8 h to a commercial abattoir in summer (6 wk) and winter (5 wk). Pig behavior was observed at loading, in transit, at unloading, and in lairage. Handler intervention at loading was observed, and the time to load and unload was recorded. Although season did not (P = 0.91) affect loading time, more prods (P = 0.014) were necessary to load pigs in summer than winter. Loading in winter also tended to be longer (P = 0.071) into compartments involving internal ramps. In transit, more pigs (P = 0.025) were standing in winter compared with summer. Unloading took longer (P < 0.006) in winter than in summer and from compartments where pigs had to negotiate ramps and 180° turns. Furthermore, pigs in summer experienced more slipping (P = 0.032), falling (P = 0.004), overlapping (P < 0.001), and walking backward (P < 0.001) than pigs in winter. Pigs unloading from compartments with internal ramps slipped more (P < 0.0001) than other pigs. Season influenced latency to rest in lairage, with those transported in summer resting sooner (P < 0.0001) than those in winter. In conclusion, season and location within trucks differentially affect pig behavior before, during, and after long-distance transportation. Differences in lighting and temperature between seasons and the inclusion of internal ramps within vehicles may play important roles in the welfare of pigs transported to slaughter.

  6. The Danish System Revisited.

    ERIC Educational Resources Information Center

    Whitehead, John S.

    The paper is a supplement to an earlier paper in the same series which reviews Danish higher education until 1977. Expansion in higher education in the last 20 years, approaching the scale of mass higher education, culminated in a crisis in 1977. At that time, a trend toward self-government and participatory governing boards was seen as the end of…

  7. The Danish Stroke Registry

    PubMed Central

    Johnsen, Søren Paaske; Ingeman, Annette; Hundborg, Heidi Holmager; Schaarup, Susanne Zielke; Gyllenborg, Jesper

    2016-01-01

    Aim of database The aim of the Danish Stroke Registry is to monitor and improve the quality of care among all patients with acute stroke and transient ischemic attack (TIA) treated at Danish hospitals. Study population All patients with acute stroke (from 2003) or TIA (from 2013) treated at Danish hospitals. Reporting is mandatory by law for all hospital departments treating these patients. The registry included >130,000 events by the end of 2014, including 10,822 strokes and 4,227 TIAs registered in 2014. Main variables The registry holds prospectively collected data on key processes of care, mainly covering the early phase after stroke, including data on time of delivery of the processes and the eligibility of the individual patients for each process. The data are used for assessing 18 process indicators reflecting recommendations in the national clinical guidelines for patients with acute stroke and TIA. Patient outcomes are currently monitored using 30-day mortality, unplanned readmission, and for patients receiving revascularization therapy, also functional level at 3 months poststroke. Descriptive data Sociodemographic, clinical, and lifestyle factors with potential prognostic impact are registered. Conclusion The Danish Stroke Registry is a well-established clinical registry which plays a key role for monitoring and improving stroke and TIA care in Denmark. In addition, the registry is increasingly used for research. PMID:27843349

  8. Tylosin depletion from edible pig tissues.

    PubMed

    Prats, C; El Korchi, G; Francesch, R; Arboix, M; Pérez, B

    2002-12-01

    The depletion of tylosin from edible pig tissues was studied following 5 days of intramuscular (i.m.) administration of 10 mg/kg of tylosin to 16 crossbreed pigs. Animals were slaughtered at intervals after treatment and samples of muscle, kidney, liver, skin+fat, and injection site were collected and analysed by high-performance liquid chromatography (HPLC). Seven days after the completion of treatment, the concentration of tylosin in kidney, skin+fat, and at the injection site was higher than the European Union maximal residue limit (MRL) of 100 microg/kg. Tylosin residues in all tissues were below the quantification limit (50 microg/kg) at 10 and 14 days post-treatment.

  9. Physico-chemical characteristics of Longissimus lumborum muscle in goats subjected to halal slaughter and anesthesia (halothane) pre-slaughter.

    PubMed

    Sabow, Azad Behnan; Sazili, Awis Qurni; Zulkifli, Idrus; Goh, Yong Meng; Ab Kadir, Mohd Zainal Abidin; Adeyemi, Kazeem Dauda

    2015-12-01

    This study assessed the effect of halal slaughter and anesthesia pre-slaughter followed by bleeding on meat quality characteristics of goats. Eleven male Boer cross goats were divided into two groups and subjected to either halal slaughter (HS) or anesthesia with halothane and propofol pre-slaughter (AS). At pre-rigor, HS had significantly lower (P < 0.05) muscle pH and glycogen than AS. However, no significant difference was observed in the pH and glycogen content between the treatments on 1, 3 and 7 days post mortem. The drip loss of HS was significantly lower (P < 0.05) than that of AS at all aging periods. Treatment had no effect on sarcomere length, myofibrillar fragmentation index and shear force values, loss of thiol groups and degradation of major myofibrillar proteins. It can be concluded that HS did not have deleterious effect on meat quality traits of goat when compared to AS.

  10. Longitudinal serological responses to Salmonella enterica of growing pigs in a subclinically infected herd.

    PubMed

    Beloeil, P A; Chauvin, C; Proux, K; Rose, N; Queguiner, S; Eveno, E; Houdayer, C; Rose, V; Fravalo, P; Madec, F

    2003-08-28

    A longitudinal survey was conducted in France in a subclinically Salmonella-infected farrow-to-finish pig farm to describe the time-course of the serological response to Salmonella enterica in growing pigs. We used three batches of sows and their corresponding litters (n = 31 litters). Among these, 256 pigs randomly selected and individually identified were followed from the first week of age until slaughter. Serial individual blood samples were submitted to indirect Salmonella-ELISA testing. Salmonella shedding was investigated by bacteriological testing of faecal material regularly collected from the sows and pigs and by environment swabs taken from the pens. Caecal contamination was checked at the slaughterhouse. Information about litter composition (filiation), location of the pigs in post-weaning and fattening pens, sanitary events, sex and body weights was recorded. 11.6% of the pigs shed S. enterica; 52% of pigs seroconverted before slaughter. The age-related variation of the natural logarithm of calibrated optical densities (COD) of pigs was described with two linear mixed models. From 8 to 61 days of age, the decrease in COD with age was fitted with a model including random effects of the animal and the dam on the intercept and slope, a batch random effect on the intercept and an individual birth-weight fixed effect on the intercept. The dam random effect was explained by the parity of the sow, Salmonella shedding by the sow during the farrowing phase and the value of the optical density of colostrum collected at parturition. A second model fitting the increase in COD from 61 days of age until slaughter included the random effect on intercept of the batch and the random effects on slope and intercept of the animal, the dam and the pen in which the followed animals were located during the fattening phase and the environmental contamination as fixed effect. In this second model, no relation was found between individual slaughter-bacteriological results and

  11. Effect of fenbendazole in water on pigs infected with Ascaris suum in finishing pigs under field conditions.

    PubMed

    Lassen, Brian; Oliviero, Claudio; Orro, Toomas; Jukola, Elias; Laurila, Tapio; Haimi-Hakala, Minna; Heinonen, Mari

    2017-03-06

    The husbandry of pigs for meat production is a constantly developing industry. Most studies on the effects of Ascaris suum infection in pigs and its prevention with anthelmintics are over a decade old. We examined the effect of 2.5mg fenbendazole per kg bodyweight administered in drinking water for two consecutive days on A. suum infection 1 and 6 weeks after pigs arrived to fattening units. We hypothesised that the treatment would reduce the presence of A. suum-infections, improve the average daily weight gain of pigs, reduce the percentage of liver rejections in pens by 50% and increase the lean meat percentage at slaughter by 1%. The study included a placebo group (427 pigs) and a treatment group (420 pigs) spanning four different farms previously reporting ≥15% liver rejection. The treatment was given for 2 consecutive days 1 and 6 weeks after the pigs arrived to the fattening unit. Faecal samples were collected during weeks 1, 6 and 12 from all pigs and examined for A. suum eggs. Blood was collected during weeks 1 and 12 from a subgroup of the pigs and examined for anti-A. suum antibodies and clinical blood parameters. Data on liver rejection and lean meat percentage were collected post-mortem. The proportion of Ascaris seropositive pigs changed from 8.6% to 22.2% and 20.3% to 16.3% in the placebo and treatment group respectively. Fenbendazole reduced the presence of A. suum eggs in faeces the percentage of liver rejections by 69.8%. The treatment did not affect daily weight gain or lean meat percentage. Pigs with A. suum eggs in faeces at week 6 had a lower average daily weight gain of 61.8g/day compared with pigs without parasite eggs. Fenbendazole treatment may be a useful option for farms struggling with persistent A. suum problems and demonstrate a beneficial effect on the weight gain of the animals shedding eggs in faeces and result in fewer condemned livers at slaughter.

  12. Can the monitoring of animal welfare parameters predict pork meat quality variation through the supply chain (from farm to slaughter)?

    PubMed

    Rocha, L M; Velarde, A; Dalmau, A; Saucier, L; Faucitano, L

    2016-01-01

    The objective of this study was to assess the relationship between the animal welfare conditions evaluated through the supply chain and pork quality variation. A total of 4,680 pigs from 12 farms-5 animal welfare improved raising system (AWIRS) and 7 conventional raising system (CON) farms-were assessed from farm to slaughter through a comprehensive audit protocol merging the European Welfare Quality, the Canadian Animal Care Assessment, and American Meat Institute audit guide criteria. At the abattoir, a subsample of 1,440 pigs (120 pigs/farm) was randomly chosen out of 24 loads (2 farms per wk) transported by 2 drivers (driver A and driver B) for the assessment of stunning effectiveness, carcass bruises, blood lactate levels, and meat quality traits. Meat quality was assessed in the longissimus lumborum (LL) muscle 24 h postmortem by measuring ultimate pH (pHu), color (L*, a*, and b*), and drip loss. Data were analyzed by the MIXED, GLIMMIX, and NAPAR1WAY procedures of SAS. Spearman correlations were calculated to determine the relationship between audit scores and meat quality traits. Better animal welfare conditions, as showed by greater final scores for good housing (GHo; = 0.001) and good health ( = 0.006) principles, were recorded at AWIRS farms. Pigs from AWIRS farms handled by driver B displayed a greater percentage of turning back ( = 0.01) and slips ( < 0.001) during unloading and a greater ( = 0.02) frequency of falls in the stunning chute. A greater ( = 0.02) reluctance to move at loading was found in CON pigs loaded by driver A compared with driver B, whereas a greater ( < 0.001) reluctance to move was found in these pigs at unloading when they were unloaded by driver B. Drip loss was higher ( = 0.003) and pale, soft, and exudative pork percentage was greater ( < 0.001) in the LL muscle of the heavier AWIRS pigs. The GHO principle was best correlated with pHu ( = -0.75, = 0.01) and Minolta L* value ( = 0.87, < 0.001) of the LL muscle. Overall, drip

  13. Effect of heat stress on blood rheology in different pigs breeds.

    PubMed

    Waltz, Xavier; Baillot, Michelle; Connes, Philippe; Gourdine, Jean-Luc; Philibert, Lucien; Beltan, Eric; Chalabi, Tawfik; Renaudeau, David

    2014-01-01

    The main objectives of the present work were to test the effects of heat stress on blood rheology and to determine whether the responses can change according to the pig breeds. Thirty-six pigs from three pig's lines (n = 12 for each line) with assumed different tolerance to heat stress were compared: Large White (LW, little tolerance), Creole (CR, good tolerance) and LW × CR pigs (produced from a cross between LW and CR lines). In a first period, all pigs were exposed to a 9-d period of thermo-neutral environment (24°C; d-9 to d-1; P0). At the end of P0, six pigs from each line were slaughtered (n = 18). Then in a second period, the remaining pigs (6/breed; n = 18) were exposed to a 5-d period of heat stress (32°C; d + 1 -d + 5; P1) and thereafter slaughtered at d + 5. Rectal and skin temperatures, as well as respiratory rate, were recorded on d-1 and d + 5. At slaughter, blood was sampled for hematological and hemorheological measurements. Heat stress caused a rise of the skin temperature and respiratory rate without any changes in the rectal temperature or on the hematological and hemorheological parameters when all pigs' lines were considered. We observed a pig line effect on blood viscosity at high shear rate (375 s-1) and red blood cell deformability at 30 Pa with CR pigs having lower blood viscosity and higher red blood cell deformability than LW pigs. While the changes of blood viscosity under heat stress did not reach statistical significance in LW and CR lines, blood viscosity (at 375 s-1) increased above the temperate values in the LW × CR line. Red blood cell deformability at 30 Pa was higher in CR pigs exposed to heat stress compared to LW pigs in the same condition. In conclusion, thermal loading caused physiological stress but did not widely change the hematological and hemorheological profiles. Although some blood rheological parameters seem to vary with the pig breeds, the responses to heat stress are very similar.

  14. Association between pigs with high caecal Salmonella loads and carcass contamination.

    PubMed

    Pesciaroli, M; Cucco, L; De Luca, S; Massacci, F R; Maresca, C; Medici, L; Paniccià, M; Scoccia, E; Staffolani, M; Pezzotti, G; Magistrali, C F

    2017-02-02

    Contaminated pork is a significant source of foodborne Salmonella infections. Pork is contaminated at the slaughterhouse; however, the mechanisms driving Salmonella contamination of carcasses are still poorly understood. The aim of this study was to investigate whether the amount of Salmonella carried by slaughtered pigs in their guts has an influence on carcass contamination. On that account, we tested whether the number of carcasses contaminated during a slaughter day was associated with the prevalence of highly contaminated pigs (HCP: Salmonella caecal loads ≥3log/g), or with the prevalence of pigs that simply carry Salmonella spp. in their guts. Three hundred and six pigs were sampled in a slaughterhouse from Central Italy. Salmonella loads in the caecum and on the carcass of each pig were estimated by the most probable number (MPN) technique. The overall prevalence of Salmonella was 34.64% and 7.19% for the caeca and carcasses, respectively. S. Derby and Salmonella enterica 4,[5],12:i:- were the most frequently isolated serovars. The prevalence of HCP was 11.44%. We found a higher number of contaminated carcasses on days of high prevalence of HCP than on days of low prevalence of HCP (p=0.0011). Conversely, carcass contamination did not vary with the prevalence of pigs that simply carried Salmonella spp. in their guts (p=0.7970). Therefore, the prevalence of HCP, but not the prevalence of pigs carrying Salmonella spp., was related to carcass contamination. Taken together, these findings suggest that reduction of Salmonella loads in the guts of slaughtered pigs would result in fewer contaminated carcasses, and consequently, help to minimise the risk of human infection due to the consumption of contaminated pork.

  15. Current food chain information provides insufficient information for modern meat inspection of pigs.

    PubMed

    Felin, Elina; Jukola, Elias; Raulo, Saara; Heinonen, Jaakko; Fredriksson-Ahomaa, Maria

    2016-05-01

    Meat inspection now incorporates a more risk-based approach for protecting human health against meat-borne biological hazards. Official post-mortem meat inspection of pigs has shifted to visual meat inspection. The official veterinarian decides on additional post-mortem inspection procedures, such as incisions and palpations. The decision is based on declarations in the food chain information (FCI), ante-mortem inspection and post-mortem inspection. However, a smooth slaughter and inspection process is essential. Therefore, one should be able to assess prior to slaughter which pigs are suitable for visual meat inspection only, and which need more profound inspection procedures. This study evaluates the usability of the FCI provided by pig producers and considered the possibility for risk ranking of incoming slaughter batches according to the previous meat inspection data and the current FCI. Eighty-five slaughter batches comprising 8954 fattening pigs were randomly selected at a slaughterhouse that receives animals from across Finland. The mortality rate, the FCI and the meat inspection results for each batch were obtained. The current FCI alone provided insufficient and inaccurate information for risk ranking purposes for meat inspection. The partial condemnation rate for a batch was best predicted by the partial condemnation rate calculated for all the pigs sent for slaughter from the same holding in the previous year (p<0.001) and by prior information on cough declared in the current FCI (p=0.02) statement. Training and information to producers are needed to make the FCI reporting procedures more accurate. Historical meat inspection data on pigs slaughtered from the same holdings and well-chosen symptoms/signs for reporting, should be included in the FCI to facilitate the allocation of pigs for visual inspection. The introduced simple scoring system can be easily used for additional information for directing batches to appropriate meat inspection procedures. To

  16. Danish auroral science history

    NASA Astrophysics Data System (ADS)

    Stauning, P.

    2011-01-01

    Danish auroral science history begins with the early auroral observations made by the Danish astronomer Tycho Brahe during the years from 1582 to 1601 preceding the Maunder minimum in solar activity. Included are also the brilliant observations made by another astronomer, Ole Rømer, from Copenhagen in 1707, as well as the early auroral observations made from Greenland by missionaries during the 18th and 19th centuries. The relations between auroras and geomagnetic variations were analysed by H. C. Ørsted, who also played a vital role in the development of Danish meteorology that came to include comprehensive auroral observations from Denmark, Iceland and Greenland as well as auroral and geomagnetic research. The very important auroral investigations made by Sophus Tromholt are outlined. His analysis from 1880 of auroral observations from Greenland prepared for the significant contributions from the Danish Meteorological Institute, DMI, (founded in 1872) to the first International Polar Year 1882/83, where an expedition headed by Adam Paulsen was sent to Greenland to conduct auroral and geomagnetic observations. Paulsen's analyses of the collected data gave many important results but also raised many new questions that gave rise to auroral expeditions to Iceland in 1899 to 1900 and to Finland in 1900 to 1901. Among the results from these expeditions were 26 unique paintings of the auroras made by the artist painter, Harald Moltke. The expedition to Finland was headed by Dan la Cour, who later as director of the DMI came to be in charge of the comprehensive international geomagnetic and auroral observations made during the Second International Polar Year in 1932/33. Finally, the article describes the important investigations made by Knud Lassen during, among others, the International Geophysical Year 1957/58 and during the International Quiet Sun Year (IQSY) in 1964/65. With his leadership the auroral and geomagnetic research at DMI reached a high international

  17. Danish Cultural Identity and the Teaching of Danish to Foreigners

    ERIC Educational Resources Information Center

    Reuter, Hedwig

    2006-01-01

    Danish as a second language textbooks published over the last 15 years have presented the Danish cultural identity as a homogenous and purely national phenomenon. Research into teaching theory, on the other hand, has been more broad-minded, and is based on interactivity. The aim of this paper is to explain this divergence. (Contains 2 notes.)

  18. Estimating the body temperature of groups of pigs by thermal imaging.

    PubMed

    Warriss, P D; Pope, S J; Brown, S N; Wilkins, L J; Knowles, T G

    2006-03-11

    Measurements on 28 pens of pigs containing 384 animals to be slaughtered at a commercial abattoir showed that the mean ear temperatures of the pigs in each pen, measured with a thermal imaging camera, were significantly correlated (r=0.71, P<0.001) with the mean temperature of the blood the pigs lost at exsanguination. In measurements on 220 pigs in 16 of the 28 pens, the mean activity of serum creatine kinase was positively correlated with the mean ear temperature (r=0.55, P<0.05) and the mean concentration of serum cortisol was positively correlated with the mean blood temperature (r=0.50, P<0.05), suggesting that the hotter pigs were suffering from a higher level of stress.

  19. The Effect of Medicated Feed on the Nasal Microflora and Weight Gain of Pigs

    PubMed Central

    Woods, G. T.; Jensen, A. H.; Gossling, Jennifer; Rhoades, H. E.; Nickelson, W. F.

    1972-01-01

    Antimicrobial agents were added to the feed of swine for three weeks to determine the interrelationships of potentially pathogenic agents in the nasal tract, turbinate atrophy and weight gains. Bordetella bronchiseptica was not isolated from the groups fed the combination of chlortetracycline, penicillin and sulfamethazine. B. bronchiseptica was found in some pigs after the feeding trail, but this organism was not significantly associated with turbinate atrophy at the time of slaughter. Mycoplasma hyorhinis was not found in the nasal passages of the pigs that received feed containing high concentration chlortetracycline but was found in pigs that received other diets. Hemophilus suis was not significantly reduced by any of the treatments used. The organisms studied in the pigs were not isolated from the personnel handling the pigs. PMID:4258545

  20. Selenium elimination in pigs after an outbreak of selenium toxicosis.

    PubMed

    Davidson-York, D; Galey, F D; Blanchard, P; Gardner, I A

    1999-07-01

    In May 1996, 150 grower pigs in 5 California counties were exposed to selenium-contaminated feed distributed by a single feed company. Feed samples from 20 herds had a mean selenium concentration of 121.7 ppm dry weight (range, 22.1-531 ppm). In San Luis Obispo County, 52 pigs in 24 herds were exposed to the feed, and 8 pigs died with signs of paralysis. Bilateral symmetrical poliomyelomalacia involving the ventral horns of the cervical and lumbar intumescence was evident on histologic examination of spinal cord from affected pigs. Of 44 surviving exposed pigs, 33 (75%) exhibited signs of selenosis, including anorexia, alopecia, and hoof lesions. Thirty-nine of 44 pigs (88.6%) had elevated (>1 ppm) blood selenium concentrations. Surviving exposed pigs were changed to a standard commercial ration containing approximately 0.5 ppm (dry weight) selenium. Blood selenium concentrations were determined weekly for 46 days following removal of the contaminated feed and were compared with values of 20 control pigs fed a standard commercial ration. Mean (+/-SD) blood selenium concentrations of exposed pigs were 3.2 +/- 2.6 ppm at the initial sampling and 0.4 +/- 0.1 ppm after 46 days. Mean blood selenium concentrations of < or = 0.3 ppm for control pigs at all samplings were significantly lower (P < 0.001) than concentrations for exposed pigs. Muscle and liver samples of 22 of the 44 exposed pigs were collected at slaughter approximately 72 days after withdrawal of the selenium-contaminated feed. Muscle samples had a mean selenium concentration of 0.36 ppm (wet weight). Liver samples had a mean selenium concentration of 1.26 ppm (wet weight). One liver sample had a selenium value in the toxic range for pigs (3.3 ppm wet weight; reference range, 0.4-1.2 ppm). A 1-compartment pharmacokinetic model of selenium elimination in exposed pigs was generated, and the geometric mean blood selenium elimination half-life was estimated to be 12 days. The 60-day withdrawal time recommended

  1. 9 CFR 88.5 - Requirements at a slaughtering facility.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Requirements at a slaughtering facility. 88.5 Section 88.5 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL...

  2. 9 CFR 88.5 - Requirements at a slaughtering facility.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Requirements at a slaughtering facility. 88.5 Section 88.5 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL...

  3. 9 CFR 88.5 - Requirements at a slaughtering facility.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Requirements at a slaughtering facility. 88.5 Section 88.5 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL...

  4. 9 CFR 88.5 - Requirements at a slaughtering facility.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Requirements at a slaughtering facility. 88.5 Section 88.5 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL...

  5. 9 CFR 88.5 - Requirements at a slaughtering facility.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Requirements at a slaughtering facility. 88.5 Section 88.5 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL...

  6. Machine vision system for online inspection of freshly slaughtered chickens

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A machine vision system was developed and evaluated for the automation of online inspection to differentiate freshly slaughtered wholesome chickens from systemically diseased chickens. The system consisted of an electron-multiplying charge-coupled-device camera used with an imaging spectrograph and ...

  7. 9 CFR 146.4 - General provisions for all participating flocks and slaughter plants.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... participating flocks and slaughter plants. 146.4 Section 146.4 Animals and Animal Products ANIMAL AND PLANT... and slaughter plants. (a) Records that establish the identity of products handled shall be maintained... classification may include in their advertising reference to associated or franchised slaughter or...

  8. 9 CFR 93.518 - Swine from Canada for immediate slaughter.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Swine from Canada for immediate slaughter. 93.518 Section 93.518 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE... immediate slaughter. Swine imported from Canada for immediate slaughter shall be consigned from the port...

  9. 9 CFR 73.2 - Interstate shipment for immediate slaughter from quarantined or nonquarantined areas; conditions...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... slaughter from quarantined or nonquarantined areas; conditions under which permitted. 73.2 Section 73.2... Interstate shipment for immediate slaughter from quarantined or nonquarantined areas; conditions under which... shipment may be shipped or transported interstate for immediate slaughter to a recognized...

  10. Airborne transmission of H5N1 high pathogenicity avian influenza viruses during simulated home slaughter

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Most H5N1 human infections have occurred following exposure to H5N1 high pathogenicity avian influenza (HPAI) virus-infected poultry, especially when poultry are home slaughtered or slaughtered in live poultry markets. Previous studies have demonstrated that slaughter of clade 1 isolate A/Vietnam/1...

  11. Antimicrobial growth promoter ban and resistance to macrolides and vancomycin in enterococci from pigs.

    PubMed

    Boerlin, P; Wissing, A; Aarestrup, F M; Frey, J; Nicolet, J

    2001-11-01

    Ninety-six enterococcus isolates from fecal samples of pigs receiving tylosin as an antimicrobial growth promoter and 59 isolates obtained in the same farms 5 to 6 months after the ban of antimicrobial growth promoters in Switzerland were tested for susceptibility to nine antimicrobial agents. A clear decrease in resistance to macrolides, lincosamides, and tetracycline was visible after the ban. Vancomycin-resistant Enterococcus faecium belonged to the same clonal lineage as vancomycin-resistant isolates previously isolated from Danish pigs.

  12. Pneumonia in Saskatchewan Swine: Abattoir Incidence of Intrathoracic Lesions in Pigs from a Herd Infected with Haemophilus pleuropneumoniae and from Other Herds

    PubMed Central

    Saunders, J. R.; Osborne, A. D.; K-Sebunya, T.

    1981-01-01

    A 1978-79 survey of the incidence of thoracic cavity lesions at slaughter had shown that the overall incidence of pleurisy in Saskatchewan swine was low (2%). Therefore, in the summer of 1979 a comparison was made between the incidence of pleurisy in a herd of pigs chronically affected with Haemophilus pleuropneumoniae pneumonia and in animals from other herds slaughtered at the same time. The incidence of pleurisy in control pigs (3.6%) was slightly higher than in the large scale survey but in the pigs from the Haemophilus infected herd it was almost four times as great (13.3%). In the same herd the survivors of a batch of pigs which had been decimated by more severe disease showed an incidence of 32% pleurisy. The economic implications of these findings are detailed and discussed. PMID:7340926

  13. Comparative growth performance of pigs immunised with a gonadotrophin releasing factor vaccine with surgically castrated pigs and entire boars raised under conventionally managed conditions.

    PubMed

    Fuchs, Thilo; Nathues, Heiko; Koehrmann, Annika; Andrews, Stuart; Brock, Fiona; Klein, Günter; grosse Beilage, Elisabeth

    2011-01-01

    The objective of this study was to determine the effect of using a gonadotrophin releasing factor (GnRF) vaccine on growth performance of male pigs. Vaccinated pigs were compared with surgically castrated pigs and entire males under conventionally managed farm conditions. Male pigs were randomly assigned to three treatment groups: pigs surgically castrated during the first week of life (group "C", n=274), pigs immunised twice during the fattening period with a GnRF vaccine, the first when 13-14 weeks of age and the second when 20-21 weeks of age (group "V" n=280) and entire males (group "E", n=56). Average daily weight gain (ADG) was measured during the study. Additionally, feed conversion ratio (FCR) as defined by feed intake/weight gain and feed consumption was assessed during the fattening period. No difference in the ADG during the fattening period could be determined between "C" and "V". From the start of the study until slaughter at study day 160/173 the ADG in group "E" was lower (625 g/day; P) than in group "C" (681 g/day; P < 0.001) and group "V" (675 g/day; P < 0.01). The FCR during the fattening period until study day 160, three days before the first batch of pigs were slaughtered at 24-25 weeks of age, was higher in group"C" (2.72 kg feed intake/kg weight gain) compared with group "V" (2.44 kg feed intake/kg weight gain; P < 0.001) and group "E" (2.44 kg feed intake/kg weight gain; P <0.01). Pigs in group "V" performed similarly to those in group "E" until the second vaccination (day 135) after which time they showed a substantial increase in feed intake and ADG, while retaining an FCR advantage over group C.

  14. Vitamin C plasma concentrations and leg weakness in the forelegs of growing pigs.

    PubMed

    Armocida, A; Beskow, P; Amcoff, P; Kallner, A; Ekman, S

    2001-04-01

    Four litters (41 pigs) of cross-bred pigs were studied from 6 to 26 weeks of age. Blood samples were collected at 6, 13, 21 and 26 weeks of age and analysed for contents of vitamin C, calcium (Ca), inorganic phosphorus (P) and alkaline phosphatase (ALP). The pigs were examined clinically for foreleg weakness at the ages of 21 and 26 weeks. At the age of 26 weeks the pigs were slaughtered and the right forelegs were examined macroscopically and selected samples were collected for radiological, histological and ultrastructural examination. The prevalence of foreleg lesions was high, with lesions of dyschondroplasia of the distal growth plate of the ulna in 30 pigs, synovitis of the elbow joint in 24 pigs and osteochondritis dissecans of the elbow joint in 25 pigs. At the ages of 21 and 26 weeks, five pigs had evidently crooked forelegs and 14 pigs (age 21 weeks) and 25 pigs (age 26 weeks) had mildly deformed forelegs. The serum levels of Ca, P and ALP were within normal values for growing-finishing pigs. The range of vitamin C concentrations in plasma showed a wide difference (7.1-49.8 mumol/l) but was not associated with deformed forelegs. The serum concentrations of Ca, P and ALP and the plasma concentration of vitamin C differed significantly (P = 0.05) between age groups and there was a significant (P = 0.001) positive correlation between the levels of vitamin C in plasma and the serum levels of ALP at 6 weeks of age. The aim of the present study was to determine if there was any association between the plasma levels of vitamin C and the extent of crooked or deviated forelegs in growing-finishing pigs. We could not find a vitamin C deficiency during the study and no association between low levels of vitamin C in plasma and the presence of deformed forelegs of these 40 pigs.

  15. The response of various muscle types to a restriction -re-alimentation feeding strategy in growing pigs.

    PubMed

    Lebret, B; Heyer, A; Gondret, F; Louveau, I

    2007-07-01

    Muscle lipid concentration is known to influence pork eating quality. This study aimed at evaluating the effect of a restriction-re-alimentation feeding strategy on intramuscular fat deposition in pigs. A total of 70 Duroc × (Large White × Landrace) pigs (castrated males and females) were used. Ten pigs were first slaughtered at 30 kg live weight (LW) to determine initial body and muscle composition. From 30 to 80 kg LW (growing period), pigs were either fed ad libitum (AL) or restricted to 70% of the ad libitum intake of AL pigs (RA). From 80 to 110 kg LW (finishing period), both AL and RA pigs were fed ad libitum. In each group, pigs were slaughtered at 80 kg (n = 10) and at 110 kg (n = 20) LW. During the growing period, the growth rate of RA pigs was reduced by 30% (P < 0.001) compared with AL pigs. During the finishing period, RA pigs had a 7% (P = 0.09) higher growth rate than AL pigs due to compensatory feed intake (+14%). Plasma insulin-like growth factor-1 concentration was lower in RA pigs at 80 kg LW, but markedly increased after re-alimentation up to the level observed in AL pigs (P < 0.001). At 80 kg, the leaner carcasses of RA pigs resulted from a more pronounced reduction in fat than in lean tissue deposition rates. Re-alimentation of RA pigs increased fat tissue deposition (+160% for females, P < 0.01) but not lean deposition in the carcass, leading to limited differences in carcass composition between RA and AL pigs at 110 kg LW. Regarding tissue deposition rates, the response to feeding strategy differs between muscles. In the m. biceps femoris (BF), restriction affected lipid (-50%, P < 0.001) and protein (-25%, P < 0.001) deposition, whereas re-alimentation increased lipid (+62%, P < 0.05) but not protein deposition rates. At market weight, the extent of the difference in BF lipid concentration between RA and AL pigs was strongly reduced, but still significant. By contrast, in the m. longissimus, restriction decreased protein but not lipid

  16. Low Prevalence of Salmonella Enterica in Cull Dairy Cattle at Slaughter in Northern Italy

    PubMed Central

    Bonardi, Silvia; Bruini, Ilaria; Magnani, Rossella; Cannistrà, Nicolò; Brindani, Franco

    2017-01-01

    In order to evaluate Salmonella carrier status of cull dairy cattle at slaughter, 125 animals were randomly selected during the period February-May 2016. Dairy cows were reared in 89 farms located in two regions of Northern Italy (Lombardy and Emilia-Romagna regions), where bovine milk is primarily used for Parmigiano-Reggiano cheese and Grana Padano cheese production. Samples were collected by swabbing a 400-cm2 area of the brisket hide and by rectoanal mucosal swabs. They were tested following the reference ISO 6579 method and the isolates were serotyped following the Kauffmann-White-Le Minor scheme and genotyped by XbaI PFGE. Salmonella was detected in 1.6% of the brisket hide samples (2/125) (95% CI: 0.4-5.6) and never found in faecal samples (95% CI: 0-3%). The positive cattle were reared in two farms located only in Emilia-Romagna region. The isolates were typed as S. Derby and S. Seftenberg. The comparison of the pulsed-field gel electrophoresis (PFGE) patterns of the bovine strains with all the PFGE patterns of the same serotypes responsible for human salmonellosis cases notified in Emilia-Romagna region in the years 2013-2015 did not find any correspondence. Therefore, the role of cull dairy cattle in transmitting Salmonella to humans seems to be less important than those of pigs and poultry in EU, but more data are needed for completing attribution source studies. PMID:28299287

  17. The Danish Heart Registry

    PubMed Central

    Özcan, Cengiz; Juel, Knud; Flensted Lassen, Jens; von Kappelgaard, Lene Mia; Mortensen, Poul Erik; Gislason, Gunnar

    2016-01-01

    Aim The Danish Heart Registry (DHR) seeks to monitor nationwide activity and quality of invasive diagnostic and treatment strategies in patients with ischemic heart disease as well as valvular heart disease and to provide data for research. Study population All adult (≥15 years) patients undergoing coronary angiography (CAG), percutaneous coronary intervention (PCI), coronary artery bypass grafting, and heart valve surgery performed across all Danish hospitals were included. Main variables The DHR contains a subset of the data stored in the Eastern and Western Denmark Heart Registries (EDHR and WDHR). For each type of procedure, up to 70 variables are registered in the DHR. Since 2010, the data quality protocol encompasses fulfillment of web-based validation rules of daily-submitted records and yearly approval of the data by the EDHR and WDHR. Descriptive data The data collection on procedure has been complete for PCI and surgery since 2000, and for CAG as of 2006. From 2000 to 2014, the number of CAG, PCI, and surgical procedures changed by 231%, 193%, and 99%, respectively. Until the end of 2014, a total of 357,476 CAG, 131,309 PCI, and 60,831 surgical procedures had been performed, corresponding to 249,445, 100,609, and 55,539 first-time patients, respectively. The DHR generally has a high level of completeness (1–missing) of each procedure (>90%) when compared to the National Patient Registry. Variables important for assessing the quality of care have a high level of completeness for surgery since 2000, and for CAG and PCI since 2010. Conclusion The DHR contains valuable data on cardiac invasive procedures, which makes it an important national monitoring and quality system and at the same time serves as a platform for research projects in the cardiovascular field. PMID:27822091

  18. The Danish Sarcoma Database

    PubMed Central

    Jørgensen, Peter Holmberg; Lausten, Gunnar Schwarz; Pedersen, Alma B

    2016-01-01

    Aim The aim of the database is to gather information about sarcomas treated in Denmark in order to continuously monitor and improve the quality of sarcoma treatment in a local, a national, and an international perspective. Study population Patients in Denmark diagnosed with a sarcoma, both skeletal and ekstraskeletal, are to be registered since 2009. Main variables The database contains information about appearance of symptoms; date of receiving referral to a sarcoma center; date of first visit; whether surgery has been performed elsewhere before referral, diagnosis, and treatment; tumor characteristics such as location, size, malignancy grade, and growth pattern; details on treatment (kind of surgery, amount of radiation therapy, type and duration of chemotherapy); complications of treatment; local recurrence and metastases; and comorbidity. In addition, several quality indicators are registered in order to measure the quality of care provided by the hospitals and make comparisons between hospitals and with international standards. Descriptive data Demographic patient-specific data such as age, sex, region of living, comorbidity, World Health Organization’s International Classification of Diseases – tenth edition codes and TNM Classification of Malignant Tumours, and date of death (after yearly coupling to the Danish Civil Registration System). Data quality and completeness are currently secured. Conclusion The Danish Sarcoma Database is population based and includes sarcomas occurring in Denmark since 2009. It is a valuable tool for monitoring sarcoma incidence and quality of treatment and its improvement, postoperative complications, and recurrence within 5 years follow-up. The database is also a valuable research tool to study the impact of technical and medical interventions on prognosis of sarcoma patients. PMID:27822116

  19. Sunlight exposure increases vitamin D sufficiency in growing pigs fed a diet formulated to exceed requirements.

    PubMed

    Alexander, B M; Ingold, B C; Young, J L; Fensterseifer, S R; Wechsler, P J; Austin, K J; Larson-Meyer, D E

    2017-04-01

    Traditional confinement practices limit exposure to sunlight and vitamin D synthesis, and vitamin insufficiency occurs even with dietary supplementation. The aim of this study was to determine the effect of limited sun exposure on serum concentration of vitamin D and the expression of vitamin D synthesizing enzymes in the liver and kidney of pigs on a vitamin D sufficient diet. White-pigmented grower pigs (29.7 ± 2.3 kg) fed 15% CP diet ad libitum providing >1,200 IU vitamin D3/kg of feed were exposed to sunlight for 1 h each day at solar noon for 14 d at the spring equinox (March pigs, n = 10) or summer solstice (June pigs, n = 5) and again before slaughter in June (March pigs) and September (June pigs). Blood for the analysis of 25(OH)D was collected before and after sunlight exposure. Traditionally housed pigs served as controls. After initial sun exposure, blood samples were collected from June pigs daily for 5 d and weekly for 8 wk to determine vitamin D3 and 25(OH)D decay, respectively. Kidney and liver samples were collected from the June pigs at slaughter after sun exposure for analysis of messenger RNA expression of vitamin D binding protein and synthesizing/degrading enzymes. Average daily gain (ADG) was not influenced (P > 0.5) by sunlight exposure. June pigs had fewer days on feed, lower (P = 0.003) ADG and were slaughtered at a lighter (P < 0.001) weight. Exposure to sunlight increased (P < 0.001) 25(OH) vitamin D for all pigs. March pigs, obtained from a Midwest producer, had lower (P < 0.001) concentration of 25(OH)D than June pigs born on-farm. Initial sunlight exposure increased serum concentration of 25(OH)D in March pigs by 200% and June pigs by 67%. Serum concentration of vitamin D3 was decreased (P < 0.05) by 72 h with 25(OH)D decreased (P < 0.05) by wk 4 after exposure. Expression of vitamin D binding protein, vitamin D synthesizing CYP2R1, CYP27A1, CYP2D25, or degrading enzyme CYP24A1 were not influenced (P ≥ 0.19) by sunlight

  20. Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil

    PubMed Central

    Miraglia, Fabiana; Moreno, Andréa Mike; Gomes, Cleise Ribeiro; Paixão, Renata; Liuson, Esequiel; Morais, Zenaide Maria; Maiorka, Paulo; Seixas, Fabiana Kömmling; Dellagostin, Odir Antonio; Vasconcellos, Silvio Arruda

    2008-01-01

    With the aim of isolating Leptospira spp., blood serum, kidney, liver and genital tract of 137 female swine (40 sows and 97 gilts) and also urine samples from 22 sows were collected in a slaughterhouse in the State of São Paulo, from April 2003 to August 2004. Four isolates were obtained from animals that presented microagglutination test (MAT) titers ≥ 100 for the serovar Pomona and one was obtained from an animal negative by MAT in which Leptospira was isolated from the liver and reproductive tract. The presence of leptospiral DNA was investigated by PCR, and positive results were found in kidneys of 11 females, liver of two, genital tract of two and urine of one of them. Nephrosis, interstitial multifocal nephritis, moderate to severe changing, hyalines cylinders and hemorrhagic focuses, hepatic and uterine horns congestion were histological lesions observed in higher frequency in animals positive for leptospira. The silver impregnation (Warthin Starry) confirmed the presence of spirochetes in renal tubules of four females with positive leptospira cultures from kidneys. The serogroup of the five isolates was identified as Pomona by cross agglutination with reference polyclonal antibodies. Molecular characterization of the isolates was carried out by variable-number tandem-repeats analysis. All the isolates revealed a pattern distinct from the L. interrogans Pomona type strain, but identical to a previously identified pattern from strains isolated in Argentina belonging to serovar Pomona. PMID:24031254

  1. Occurrence and severity of lung lesions in slaughter pigs vaccinated against Mycoplasma hyopneumoniae with different strategies.

    PubMed

    Hillen, Sonja; von Berg, Stephan; Köhler, Kernt; Reinacher, Manfred; Willems, Hermann; Reiner, Gerald

    2014-03-01

    Different vaccination strategies against Mycoplasma hyopneumoniae have been adopted worldwide. Reports from the field indicate varying levels of protection among currently available vaccines. The goal of the present study was to compare the efficacies of three widespread commercial vaccination strategies against M. hyopneumoniae under field conditions. 20 farms were included. 14 farms used different single dose vaccines (vaccine 1 [V1], 8 herds; vaccine 2 [V2], 6 herds); another 6 farms (V3) used a two dose vaccination strategy. Gross lesions of 854 lungs and histopathology from 140 lungs were quantified, and a quantitative PCR was applied to detect M. hyopneumoniae and porcine circovirus 2 (PCV2) DNA in lung tissue (n=140). In addition, porcine reproductive and respiratory disease virus (PRRSV), swine influenza virus (SIV), Actinobacillus pleuropneumoniae, Haemophilus parasuis and Pasteurella multocida were tested by qualitative PCR. 53% of lungs were positive for M. hyopneumoniae. 55.9% of lungs showed macroscopic enzootic pneumonia (EP)-like lesions. Lung lesion scores (P<0.001) and M. hyopneumoniae-loads (P<0.008) differed significantly among the vaccination groups, with the most severe cases and highest amounts occurring in V1. Histological alterations differed (P<0.001) between V1 and V3. Lung lesion scores and histopathological changes were significantly correlated, with prevalence and load of M. hyopneumoniae indicating that the applied diagnostic tools are valuable in confirming the prevalence and severity of M. hyopneumoniae infections. Comparing different vaccination strategies against M. hyopneumoniae indicates varying levels of protection. M. hyopneumoniae is still a major problem despite the widely applied vaccination.

  2. Shiga toxigenic and atypical enteropathogenic Escherichia coli in the feces and carcasses of slaughtered pigs.

    PubMed

    Borges, Clarissa Araújo; Beraldo, Lívia Gerbasi; Maluta, Renato Pariz; Cardozo, Marita Vedovelli; Guth, Beatriz Ernestina Cabilio; Rigobelo, Everlon Cid; de Ávila, Fernando Antônio

    2012-12-01

    Escherichia coli is a pathogen of major importance in swine and public health. To determine the prevalence of Shiga toxigenic E. coli (STEC) and enteropathogenic E. coli (EPEC), samples were collected from the feces and carcasses of swines. In total, 441 samples were collected in four samplings, of which 141 samples tested positive for either the stx1, stx2, and/or eae genes. From the positive samples, one STEC and 15 atypical EPEC (aEPEC) isolates were obtained, and all originated from the same sampling. In addition to eae, lpfA(O157/OI-141), ehxA, toxB, and lpfA(O113) were present in the aEPEC isolates. The only stx2-containing isolate carried stx2e and belonged to serotype O103:HNT. Resistance to four or more antimicrobials was found in almost half of the isolates, and some isolates shared the same fingerprint patterns by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). The presence of certain virulence genes and the high level of resistance to antimicrobials, as well as the possible fecal contamination of carcasses showed that some of the isolates are of public health concern.

  3. Selective mobilization of fatty acids in adipose tissue of heavy pigs.

    PubMed

    Bochicchio, D; Comellini, M; Lambertini, P; Marchetto, G; Della Casa, G

    2015-01-01

    The mobilization of fatty acids during food deprivation is a selective process studied in different species (humans, rodents, birds, viverrids). The aim of this work was to study the effect of fasting on selective mobilization in commercial pigs. A total of 16 barrows (Large White×Landrace (167 kg±12.5 kg live weight) were subdivided into two homogeneous groups, one subjected to 12 h and the other to 60 h of fasting (fasting time) before slaughtering. For each pig inner and outer backfat layer were sampled at slaughter and at ham trimming 24 h later (sampling time). Increasing the fasting time and the sampling time after slaughter caused an increase in the amount of free fatty acids in both layers. Therefore it can be argued that during fasting lipolysis is stimulated and remains active also after slaughtering. The factors that stimulate lipolysis determine a greater mobilization of unsaturated fatty acids than saturated ones. Thus fasting time may influence the suitability of pork for processing and conservation, since free fatty acids are more suitable for oxidation than the esterified ones.

  4. 9 CFR 313.50 - Tagging of equipment, alleyways, pens, or compartments to prevent inhumane slaughter or handling...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ..., or compartments to prevent inhumane slaughter or handling in connection with slaughter. 313.50... compartments to prevent inhumane slaughter or handling in connection with slaughter. When an inspector observes... establishment operator of the incident and request that the operator take the necessary steps to prevent...

  5. 9 CFR 313.50 - Tagging of equipment, alleyways, pens, or compartments to prevent inhumane slaughter or handling...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ..., or compartments to prevent inhumane slaughter or handling in connection with slaughter. 313.50... compartments to prevent inhumane slaughter or handling in connection with slaughter. When an inspector observes... establishment operator of the incident and request that the operator take the necessary steps to prevent...

  6. 9 CFR 313.50 - Tagging of equipment, alleyways, pens, or compartments to prevent inhumane slaughter or handling...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ..., or compartments to prevent inhumane slaughter or handling in connection with slaughter. 313.50... compartments to prevent inhumane slaughter or handling in connection with slaughter. When an inspector observes... establishment operator of the incident and request that the operator take the necessary steps to prevent...

  7. Effects of different moving devices at loading on stress response and meat quality in pigs.

    PubMed

    Correa, J A; Torrey, S; Devillers, N; Laforest, J P; Gonyou, H W; Faucitano, L

    2010-12-01

    Although there is increasing evidence regarding the negative welfare and meat quality implications of electric prod use for slaughter-weight pigs, this handling tool continues to be used. Therefore, the behavioral and physiological response and carcass and meat quality of 360 pigs being loaded onto a truck for transportation to slaughter according to 3 handling procedures were studied. The 3 handling procedures were 1) moving with an electric prod and board from the finishing pen to the truck (EP); 2) moving with a board and a paddle from the finishing pen to the truck (PAD); 3) moving with a board and a paddle from the finishing pen and using a compressed air prod in the ramp before going into the truck (CAP). A subpopulation of 144 pigs (48 pigs/treatment) was equipped with heart rate monitors. Blood samples were collected from the same animals at exsanguination for the analysis of creatine phosphokinase and lactate. Data were analyzed using an ANOVA for factorial design, with the animal as the experimental unit. Behavior was analyzed with MIXED model procedure with treatment as a fixed effect. During loading, EP pigs slipped and fell (P < 0.001) and overlapped (P = 0.03) more often, but stopped (P < 0.001) and attempted turns (P = 0.01) less often than CAP or PAD. With CAP, pigs made more 180° turns (P = 0.01) than with PAD or EP. Loading with EP led to more and longer vocalizations (P = 0.02 and P = 0.001, respectively) than loading with CAP or PAD. Loading took longest with CAP and was quickest with EP (P = 0.01). Pigs handled with EP had a greater heart rate than those moved with PAD and CAP at loading (P < 0.001), wait at loading (P < 0.001), at unloading (P = 0.05), and in lairage (P = 0.02). Pigs loaded with EP had greater (P = 0.05) lactate concentrations in blood at exsanguination compared with pigs handled with CAP, with pigs loaded with PAD being intermediate. Furthermore, ultimate pH values in the semimembranosus and adductor muscles of EP pigs were

  8. Primary Postnatal Dorsal Root Ganglion Culture from Conventionally Slaughtered Calves

    PubMed Central

    Fadda, A.; Bärtschi, M.; Hemphill, A.; Widmer, H. R.; Zurbriggen, A.; Perona, P.; Vidondo, B.; Oevermann, A.

    2016-01-01

    Neurological disorders in ruminants have an important impact on veterinary health, but very few host-specific in vitro models have been established to study diseases affecting the nervous system. Here we describe a primary neuronal dorsal root ganglia (DRG) culture derived from calves after being conventionally slaughtered for food consumption. The study focuses on the in vitro characterization of bovine DRG cell populations by immunofluorescence analysis. The effects of various growth factors on neuron viability, neurite outgrowth and arborisation were evaluated by morphological analysis. Bovine DRG neurons are able to survive for more than 4 weeks in culture. GF supplementation is not required for neuronal survival and neurite outgrowth. However, exogenously added growth factors promote neurite outgrowth. DRG cultures from regularly slaughtered calves represent a promising and sustainable host specific model for the investigation of pain and neurological diseases in bovines. PMID:27936156

  9. Animal Welfare Evaluation at a Slaughterhouse for Heavy Pigs Intended for Processing

    PubMed Central

    Mandolini, Nicholas Aconiti; Marinsalti, Maria; Cammertoni, Natalina; Loschi, Anna Rita; Rea, Stefano

    2014-01-01

    The Council Regulation (EC) No. 1099/2009 requires slaughterhouse managers to implement specific standard operating procedures for all pre-slaughter stages considered at risk, aimed at achieving adequate levels of animal welfare. This survey was aimed at testing the applicability to an abattoir for heavy pigs of an assessment system of animal welfare through animal-based measures. In the monitoring of handling operations, the number of animals fallen/slipped and prodded, and that of vocalising pigs were recorded. In the monitoring of the immobilisation stage, carried out on the same pigs, vocalisations were recorded at the entrance to the box and falls/slips occurring inside it. Animal welfare assessment during the stunning-sticking-bleeding steps, was carried out by recording the head-only electrical stunning basic parameters set by legislation, vocalisations resulting from hot wanding, and clinical signs of consciousness, sensibility and certain death. Except for immobilisation, the percentage of occurrence of these events above acceptability limits was detected in all other pre-slaughter steps. The most critical stages were: handling in the unloading area and along the single-file chute, stunning and especially bleeding, where 84.13% of animals showed one or more signs of consciousness and/or sensibility recovery. Wrong placement of electrodes observed in 53.98% of the animals, insufficient voltage and low amperage may explain why a high percentage of pigs recovered consciousness and/or sensibility before death. Some simple restructuring of unloading area, slowdown of slaughter line speed, increase of personnel involved in pre-slaughter management and regular calibration of the electrical stunning device could be effectively corrective measures aimed at raising the animal welfare level at the slaughterhouse under study. PMID:27800319

  10. mcr-1−Harboring Salmonella enterica Serovar Typhimurium Sequence Type 34 in Pigs, China

    PubMed Central

    Yi, Linxian; Wang, Jing; Gao, Yanling; Liu, Yiyun; Doi, Yohei; Wu, Renjie; Zeng, Zhenling; Liang, Zisen

    2017-01-01

    We detected the mcr-1 gene in 21 (14.8%) Salmonella isolates from pigs at slaughter; 19 were serovar Typhimurium sequence type 34. The gene was located on IncHI2-like plasmids that also harbored IncF replicons and lacked a conjugative transfer region. These findings highlight the need to prevent further spread of colistin resistance in animals and humans. PMID:28098547

  11. Critical limits for the control points for halal poultry slaughter.

    PubMed

    Shahdan, Intan Azura; Regenstein, Joe Mac; Rahman, Mohammad Tariqur

    2016-12-13

    This study proposes critical limits (CL) for control points for halal slaughter (CPHS). Previously, 6 control points (CP) were determined, and CL for these 6 CPHS are suggested based on: 1) a literature survey for the CL for CP 1 (poultry breeding, rearing, and poultry feed) and CP 2 (welfare of poultry during transportation and lairage); 2) a field survey of slaughter plants in Kuantan (Malaysia) for CP 3 (immobilization), CP 4 (slaughter), CP 5 (time for full bleed-out), and CP 6 (washing and packaging); and 3) controlled experiments to refine the CL for CP 3, 4, and 5. The CL for CP 1 focused on stress reduction during rearing and use of substances that could compromise poultry meat wholesomeness. The CL for CP 2 emphasizes humane best-practices for handling poultry during lairage. The CL for CP 3 suggests a gap of 5 s between 2 shackles if only one shackler is employed and shackling times of <1 min for live chickens. In countries permitting water-bath electrical stunning of halal poultry, the stunning current needed to induce unconsciousness must be defined for the breed and bird size but not cause any chicken deaths. The CL for CP 4 mandates the recitation of the tasmiyah (the invocation), which if done for every chicken, will require ≥5 s between stunning and neck cutting. The CL for CP 4 also includes information about the slaughter knife. In CP 5 the recommended minimum time between neck cutting and scalding is 9.5 min. Finally, the CL for CP 6 emphasizes good supply chain hygiene and zero adulteration from haram species and substances.

  12. Models to quantify excretion of dry matter, nitrogen, phosphorus and carbon in growing pigs fed regional diets.

    PubMed

    Jørgensen, Henry; Prapaspongsa, Trakarn; Vu, Van Thi Khanh; Poulsen, Hanne Damgaard

    2013-11-09

    Modern pig production contributes to many environmental problems that relate to manure, especially in areas with highly intensive production systems and in regions like Asia where the regulative control is not effective. Therefore, the objective of this study was to use three different pig diets varying in dietary protein, fibre and fat as representative for Danish (DK), Thai (TH) and Vietnamese (VN) pig production to develop and evaluate different approaches to predict/calculate excretion from growing pigs in comparison with the experimentally determined values.Nine female growing pigs were used in a digestibility and balance experiment. Excretion of dry matter (DM), nitrogen (N), phosphorus (P) and carbon (C) of the experimental diets were determined.Due to the highest dietary fibre content, VN had the lowest digestibility of N, P and C (73, 49, and 73%, respectively) compared with the DK and TH pig diets. From the known diet composition using standard table values on chemical and nutrient digestibly, high accuracy (bias) and low variation was found and the results could be used for prediction on chemical composition and excretion in faeces and urine in growing pigs. Calculation based on standard values regarding nutrient retention in the pig body as used in the Danish manure normative system (DMNS) showed likewise to be quite useful for quantifying the total excretion of N and P.Overall, the results demonstrate that simple models that require cheap and normally available information on dietary nutrients can give useful information on nutrient excretion in growing pigs.

  13. Models to quantify excretion of dry matter, nitrogen, phosphorus and carbon in growing pigs fed regional diets

    PubMed Central

    2013-01-01

    Modern pig production contributes to many environmental problems that relate to manure, especially in areas with highly intensive production systems and in regions like Asia where the regulative control is not effective. Therefore, the objective of this study was to use three different pig diets varying in dietary protein, fibre and fat as representative for Danish (DK), Thai (TH) and Vietnamese (VN) pig production to develop and evaluate different approaches to predict/calculate excretion from growing pigs in comparison with the experimentally determined values. Nine female growing pigs were used in a digestibility and balance experiment. Excretion of dry matter (DM), nitrogen (N), phosphorus (P) and carbon (C) of the experimental diets were determined. Due to the highest dietary fibre content, VN had the lowest digestibility of N, P and C (73, 49, and 73%, respectively) compared with the DK and TH pig diets. From the known diet composition using standard table values on chemical and nutrient digestibly, high accuracy (bias) and low variation was found and the results could be used for prediction on chemical composition and excretion in faeces and urine in growing pigs. Calculation based on standard values regarding nutrient retention in the pig body as used in the Danish manure normative system (DMNS) showed likewise to be quite useful for quantifying the total excretion of N and P. Overall, the results demonstrate that simple models that require cheap and normally available information on dietary nutrients can give useful information on nutrient excretion in growing pigs. PMID:24206677

  14. Growth performance and carcass characteristics of Improvac-treated male pigs compared with barrows.

    PubMed

    Albrecht, Anna; Grosse Beilage, Elisabeth; Henning, Martina; Bekendorf, Torsten; Krieter, Joachim

    2012-01-01

    The present study assessed the effects of using Improvac (Pfizer Animal Health, Germany) on growth performance and meat characteristics in cross-bred male pigs. In addition average daily weight gain of immunised and surgically castrated males was estimated in order to demonstrate differences in growing patterns in these two treatment groups. The study was carried out in two identical batches. Pigs within batches (n = 446) were allocated to two different treatment groups: immunisation (IM) versus surgical castration (CM). Within treatment groups pigs were randomly assigned to two different diets (low- and high-lysine diet) in order to examine interactions of growth performance and castration technique. Vaccination was performed on the day of entry to the fattening unit and four to six weeks prior to slaughter. At the second vaccination IM-pigs showed significantly lower body weights than the control group (p < 0.05) in both batches. Throughout the whole fattening period CM-pigs tended to have a higher feed intake than IM-pigs while IM-pigs had a significantly lower (better) feed conversion ratio than CM-pigs (p < 0.05).The different lysine content of the diets had no coherent effect on any of the growth performance parameters analysed. Immunised pigs of both feeding groups and in both batches showed a tendency towards a lower carcass weight, back fat values and dressing percentages than surgically castrated pigs.The castration technique had no significant effect on meat quality parameters such as drip loss, shear force or cooking loss while intramuscular fat content (IMF) in immunised pigs fed high-lysine diets (IM(high)) tended to be higher than IMF in immunised pigs fed low-lysine diets (IM(low)). This effect was not seen in surgical castrates in either batch. Immunisation against GnRH offers a good approach to produce taint-free pork while ensuring boar-like growth for a large part of the fattening period.

  15. The slaughter of increased numbers of pregnant cows in Tanga abattoir, Tanzania: A cause for concern?

    PubMed

    Swai, Emmanuel S; Hayghaimo, Abdu A; Hassan, Ayubu A; Mhina, Bartholomeo S

    2015-08-12

    Information on the level of foetal wastage in slaughtered cattle in Tanzania is limited. A three-month observational study (April - June 2014) of animals slaughtered at the Tanga abattoir in Tanga region, Tanzania was carried out to determine the number of pregnant cows slaughtered. The total number of cattle slaughtered during the study period was 3643, representing a monthly kill average of 1214 and a daily kill average of 40. Over 98% of the cattle presented to the abattoir for slaughter were local breed (Tanzania shorthorn zebu) and most were above 3 years of age. Improved breeds of cattle represented only 1.3% of all slaughters. Of the cattle slaughtered, 2256 (61.9%) were female and 1387 (38.1%) were male. A total of 655 slaughtered cows were pregnant, representing a foetal wastage of 29.1%. Of the 655 recovered foetuses, 333 (50.8%) were male and 322 (49.2%) were female. Of the recovered foetuses, 25.8% were recovered in the first, 42.7% in the second and 31.6% in the third trimester. This study indicates cases of significant foetal losses, negatively impacting future replacement stock as a result of the slaughter of pregnant animals. The indiscriminate slaughter of pregnant cows suggests that existing animal welfare legislation is not sufficiently enforced and routine veterinary ante-mortem inspection of trade animals is failing to prevent the high level of foetal wastage.

  16. The Effect of Lupinus albus on Growth Performance, Body Composition and Satiety Hormones of Male Pigs Immunized against Gonadotrophin Releasing Factor

    PubMed Central

    Moore, Karen; Mullan, Bruce; Kim, Jae Cheol; Dunshea, Frank

    2017-01-01

    Simple Summary Pigs immunized against gonadotrophin releasing factor (immunocastrates; IC males) have an increased feed intake, growth rate, back fat and fat deposition compared to entire males. A previous experiment found that Lupinus albus L. (albus lupins) has the potential to reduce feed intake and fat deposition in IC males. The current experiment aimed to develop a dietary management strategy using albus lupins for either 14 or 28 days pre-slaughter to reduce the increase in feed intake and subsequent increase in carcass fatness in IC males. Abstract Two hundred and ninety four pigs were used with the aim to develop a dietary management strategy using Lupinus albus L. (albus lupins) to reduce the increase in feed intake and subsequent increase in carcass fatness in pigs immunized against gonadotrophin releasing factor (immunocastrates; IC males) and entire male pigs in the late finishing stage. From day (d) 0 to 28, IC males fed the control diet grew faster (p = 0.009) than entire males fed the control diet but there was no difference in growth rate between sexes for pigs fed albus lupins for 14 days pre-slaughter (Albus 14) or pigs fed albus lupins for 28 days pre-slaughter (Albus 28). From d 15 to 28, IC males receiving the Albus 14 diet grew more slowly (p < 0.001) than entire males receiving the Albus 14 diet. From d 15 to 28 (p < 0.001), IC males fed the control diet ate more feed than entire males fed the control diet, although there was no difference between sexes in feed intake of the Albus 14 and Albus 28 diet. Immunocastrates had a lower backfat when fed either Albus 14 or Albus 28 compared to the control diet, although there was no difference between diets for entire males. There was also a trend for pigs on the Albus 14 and Albus 28 diets to have a higher lean deposition (p = 0.055) and a lower fat deposition (p = 0.056) compared to the pigs on the control diet. Pigs fed the Albus 28 diet had a lower plasma ghrelin concentration compared to pigs

  17. A comparison of bleeding efficiency, microbiological quality and lipid oxidation in goats subjected to conscious halal slaughter and slaughter following minimal anesthesia.

    PubMed

    Sabow, A B; Sazili, A Q; Zulkifli, I; Goh, Y M; Ab Kadir, M Z A; Abdulla, N R; Nakyinsige, K; Kaka, U; Adeyemi, K D

    2015-06-01

    The study assessed the effect of conscious halal slaughter and slaughter following minimal anesthesia on bleeding efficiency of goats and keeping quality of goat meat. Ten Boer cross bucks were divided into two groups and subjected to either halal slaughter without stunning (HS) or minimal anesthesia prior to slaughter (AS). The blood lost during exsanguination was measured. Residual blood was further quantified by determination of hemoglobin and myoglobin content in longissimus lumborum muscle. Storage stability of the meat was evaluated by microbiological analysis and lipid oxidation. Blood loss at exsanguination, residual hemoglobin and lipid oxidation were not significantly different (p>0.05) between HS and AS. Lactic acid bacteria was the only microbe that was significantly elevated after 24h of storage at 4°C in the AS group. In conclusion, slaughtering goats under minimal anesthesia or fully conscious did not affect bleeding efficiency and keeping quality of goat meat.

  18. Herd-level risk factors for Salmonella enterica subsp. enterica in U.S. market pigs.

    PubMed

    Bahnson, P B; Fedorka-Cray, P J; Ladely, S R; Mateus-Pinilla, N E

    2006-10-17

    Midwest U.S. herds (n=63) were studied to identify risk factors for harboring Salmonella enterica among slaughter-weight pigs. Samples collected on farms (feces) and at slaughter (distal colonic content, cecal content and ileocolic lymph nodes) were cultured using conventional means. Approximately 15 pigs were studied per herd, for a total of 3754 samples. The proportion of pigs positive in one or more samples was calculated for each herd. Herd characteristics were described by a combination of interview and written survey. Logistic regression was used to detect relationships between the detection of Salmonella and potential herd-level risk factors. The mean individual pig prevalence was 5% for feces, 4% for distal colonic content, 15% for ileocolic lymph nodes, and 17% for cecal contents. One or more Salmonella isolates were detected in at least one sample type in every herd. The five most common serovars were S. Agona, S. Derby, S. Schwarzengrund, S. Typhimurium and S. Senftenberg, with 25 additional serovars detected. Salmonella prevalence estimates were positively correlated among all samples except distal colonic content and ileocolic lymph nodes. Pigs with culture positive fecal samples were at increased odds of being detected positive for each of the slaughter-collected samples examined, namely distal colonic content (OR=30.5), ileocolic lymph nodes (OR=12.9) and cecal content (OR=23.2). Herds with positive fecal sample(s) had increased odds of having positive cecal content (OR>1.5), distal colonic content (OR=15.3) and ileocolic lymph nodes (OR=12.7). Pigs from herds with at least some bowl drinkers had eight-fold higher odds of testing Salmonella positive than did pigs from herds with only nipple drinkers. Pigs from herds with only dry feeders had five-fold higher odds of testing Salmonella positive when compared with pigs from herds with combinations of wet/dry style feeders. Interventions at these two points should be considered when designing growing

  19. Danish Urogynaecological Database

    PubMed Central

    Hansen, Ulla Darling; Gradel, Kim Oren; Larsen, Michael Due

    2016-01-01

    The Danish Urogynaecological Database is established in order to ensure high quality of treatment for patients undergoing urogynecological surgery. The database contains details of all women in Denmark undergoing incontinence surgery or pelvic organ prolapse surgery amounting to ~5,200 procedures per year. The variables are collected along the course of treatment of the patient from the referral to a postoperative control. Main variables are prior obstetrical and gynecological history, symptoms, symptom-related quality of life, objective urogynecological findings, type of operation, complications if relevant, implants used if relevant, 3–6-month postoperative recording of symptoms, if any. A set of clinical quality indicators is being maintained by the steering committee for the database and is published in an annual report which also contains extensive descriptive statistics. The database has a completeness of over 90% of all urogynecological surgeries performed in Denmark. Some of the main variables have been validated using medical records as gold standard. The positive predictive value was above 90%. The data are used as a quality monitoring tool by the hospitals and in a number of scientific studies of specific urogynecological topics, broader epidemiological topics, and the use of patient reported outcome measures. PMID:27826217

  20. The effects of R-salbutamol on growth, carcass measures, and health of finishing pigs.

    PubMed

    Marchant-Forde, J N; Lay, D C; Marchant-Forde, R M; McMunn, K A; Richert, B T

    2012-11-01

    A pure form of salbutamol has the potential to deliver positive production benefits to the swine industry. The aim of this experiment was to determine the effects of salbutamol on growth, carcass measures, and health of finishing pigs. The study used 192 pigs (89 ± 1 kg BW) housed in groups of 6 in 32 pens and assigned to 1 of 4 treatments: 1) control (CTL), 0 mg/kg salbutamol; 2) 2R, control diet with 2 mg/kg of the pure R-enantiomer of salbutamol; 3) 4R, control diet with 4 mg/kg of pure R-salbutamol; or 4) 8RS, control diet with 8 mg/kg of a 50:50 mixture of the R- and S-enantiomers. All diets were offered ad libitum for 4 wk. All pigs were weighed and pen feed intakes were recorded weekly. At slaughter, individual HCW and measurements of the 10th-rib loin muscle area (LMA), color, marbling, firmness, and back fat, last lumbar, and midline back fat depths were collected. Data were analyzed using Proc GLM of SAS, with pen as the experimental unit. Overall, 2R and 4R pigs had greater ADG than CTL pigs (P < 0.05) and, at slaughter, were heavier than CTL pigs (P < 0.01). Overall, 8RS pigs had decreased ADFI (P < 0.05), and CTL pigs had poorer G:F (P < 0.001) than the other 3 treatments. All salbutamol-fed pigs had 5 to 6 kg greater HCW (P < 0.001), 2% to 3% increased carcass yield (P < 0.001), 5.6 cm(2) larger LMA (P < 0.01), 3 to 4 mm less 10th-rib back fat (P < 0.01), and 2 mm less lumbar back fat (P < 0.05) than CTL pigs. However, control pigs had greater loin muscle color scores (P < 0.05) and marbling scores (P < 0.001) than all salbutamol-treated pigs. Taken together, these data indicate that as little as 2 mg/kg R-salbutamol has a positive effect on pig growth and carcass composition. However, the effects of salbutamol on meat quality require further research.

  1. Hand-held lactate analyzer as a tool for the real-time measurement of physical fatigue before slaughter and pork quality prediction.

    PubMed

    Rocha, L M; Dionne, A; Saucier, L; Nannoni, E; Faucitano, L

    2015-04-01

    The objectives of this study were to assess the relationship between blood lactate variation measured at the plant, and pork quality variation on a large sample size and under commercial preslaughter handling conditions. A total of 600 pigs were randomly chosen on arrival at a commercial slaughter plant and blood samples taken from the ear vein at unloading (UN), after lairage (LA), in the restrainer (RE; before stunning) and at exsanguination (EX) were analysed for lactate content using a Lactate Scout Analyzer (LSA). In order to have a large range of measures, pigs were distributed into two groups; one kept in lairage overnight (G1) and the other for 2 to 3 h (G2) before slaughter. Meat quality was assessed in the Longissimus thoracis (LT), Semimembranosus (SM) and Adductor (AD) muscles by measuring the pH 30 min postmortem (pH1) and at 24 h postmortem (pHu), the colour and the drip loss. Blood lactate levels did not differ between G1 and G2 (P>0.05). A reduced muscle lactate and glucose contents (P=0.02 and P=0.004, respectively) resulting in a lower (P<0.001) glycolytic potential (GP) was observed in the LT muscle of G1 pigs when compared with G2 loins. In the LT muscle of G1 pigs, the lower GP resulted in an increased pHu (r=-0.67; P<0.001), decreased drip loss (r=0.57; P<0.001) and darker colour (r=0.50; P<0.001) compared with G2. In both G1 and G2 pigs, the lower GP was correlated to higher pHu value in the SM and AD muscles (r=-0.73; P<0.001). The greatest correlation was observed in G2 between blood lactate levels at LA and pHu value of the SM and AD muscles (r=0.46 and r=0.44, respectively; P<0.001 for both muscles). The second greatest correlation was found between blood lactate levels at EX and pH1 value in the SM muscle in both groups (r=-0.37 and r=-0.41, respectively; P<0.001 for both groups). Based on the results of this study, it appears that blood lactate levels, as measured by the LSA, reliably reflect the physiological response of pigs to

  2. Longitudinal study of distributions of similar antimicrobial-resistant Salmonella serovars in pigs and their environment in two distinct swine production systems.

    PubMed

    Keelara, Shivaramu; Scott, H Morgan; Morrow, William M; Gebreyes, Wondwossen A; Correa, Maria; Nayak, Rajesh; Stefanova, Rossina; Thakur, Siddhartha

    2013-09-01

    The aim of this longitudinal study was to determine and compare the prevalences and genotypic profiles of antimicrobial-resistant (AR) Salmonella isolates from pigs reared in antimicrobial-free (ABF) and conventional production systems at farm, at slaughter, and in their environment. We collected 2,889 pig fecal and 2,122 environmental (feed, water, soil, lagoon, truck, and floor swabs) samples from 10 conventional and eight ABF longitudinal cohorts at different stages of production (farrowing, nursery, finishing) and slaughter (postevisceration, postchill, and mesenteric lymph nodes [MLN]). In addition, we collected 1,363 carcass swabs and 205 lairage and truck samples at slaughter. A total of 1,090 Salmonella isolates were recovered from the samples; these were isolated with a significantly higher prevalence in conventionally reared pigs (4.0%; n = 66) and their environment (11.7%; n = 156) than in ABF pigs (0.2%; n = 2) and their environment (0.6%; n = 5) (P < 0.001). Salmonella was isolated from all stages at slaughter, including the postchill step, in the two production systems. Salmonella prevalence was significantly higher in MLN extracted from conventional carcasses than those extracted from ABF carcasses (P < 0.001). We identified a total of 24 different serotypes, with Salmonella enterica serovar Typhimurium, Salmonella enterica serovar Anatum, Salmonella enterica serovar Infantis, and Salmonella enterica serovar Derby being predominant. The highest frequencies of antimicrobial resistance (AR) were exhibited to tetracycline (71%), sulfisoxazole (42%), and streptomycin (17%). Multidrug resistance (resistance to ≥ 3 antimicrobials; MDR) was detected in 27% (n = 254) of the Salmonella isolates from the conventional system. Our study reports a low prevalence of Salmonella in both production systems in pigs on farms, while a higher prevalence was detected among the carcasses at slaughter. The dynamics of Salmonella prevalence in pigs and carcasses were

  3. Blood parameters and electroencephalographic responses of goats to slaughter without stunning.

    PubMed

    Sabow, A B; Goh, Y M; Zulkifli, I; Sazili, A Q; Kaka, U; Kadi, M Z A Ab; Ebrahimi, M; Nakyinsige, K; Adeyemi, K D

    2016-11-01

    The study compared changes in blood biochemistry, hormonal and electroencephalographic indices associated with possible noxious stimuli following neck cut slaughter in conscious, non-anaesthetized versus minimally-anaesthetized goats. Ten male Boer crossbreed goats were assigned into two groups and subjected to either slaughter conscious without stunning (SWS) or slaughter following minimal anaesthesia (SMA). Hormonal responses and changes in electroencephalographic (EEG) parameters were not influenced by slaughter method. The SWS goats had higher glucose and lactate than did SMA goats. It can be concluded that the noxious stimulus from the neck cut is present in both conscious and minimally anaesthetized goats. The application of slaughter without stunning causes changes in the EEG activities that are consistent with the presence of post slaughter noxious sensory input associated with tissue damage and would be expected to be experienced as pain in goats.

  4. Variation in the Antimicrobial Susceptibility of Actinobacillus pleuropneumoniae Isolates in a Pig, Within a Batch of Pigs, and Among Batches of Pigs from One Farm.

    PubMed

    Dayao, Denise Ann E; Dawson, Susan; Kienzle, Marco Jean-Paul; Gibson, Justine S; Blackall, Patrick J; Turni, Conny

    2015-08-01

    Antimicrobial resistance in bacterial porcine respiratory pathogens has been shown to exist in many countries. However, little is known about the variability in antimicrobial susceptibility within a population of a single bacterial respiratory pathogen on a pig farm. This study examined the antimicrobial susceptibility of Actinobacillus pleuropneumoniae using multiple isolates within a pig and across the pigs in three different slaughter batches. Initially, the isolates from the three batches were identified, serotyped, and subsample genotyped. All the 367 isolates were identified as A. pleuropneumoniae serovar 1, and only a single genetic profile was detected in the 74 examined isolates. The susceptibility of the 367 isolates of A. pleuropneumoniae to ampicillin, tetracycline and tilmicosin was determined by a disc diffusion technique. For tilmicosin, the three batches were found to consist of a mix of susceptible and resistant isolates. The zone diameters of the three antimicrobials varied considerably among isolates in the second sampling. In addition, the second sampling provided statistically significant evidence of bimodal populations in terms of zone diameters for both tilmicosin and ampicillin. The results support the hypothesis that the antimicrobial susceptibility of one population of a porcine respiratory pathogen can vary within a batch of pigs on a farm.

  5. Danish Palliative Care Database

    PubMed Central

    Groenvold, Mogens; Adsersen, Mathilde; Hansen, Maiken Bang

    2016-01-01

    Aims The aim of the Danish Palliative Care Database (DPD) is to monitor, evaluate, and improve the clinical quality of specialized palliative care (SPC) (ie, the activity of hospital-based palliative care teams/departments and hospices) in Denmark. Study population The study population is all patients in Denmark referred to and/or in contact with SPC after January 1, 2010. Main variables The main variables in DPD are data about referral for patients admitted and not admitted to SPC, type of the first SPC contact, clinical and sociodemographic factors, multidisciplinary conference, and the patient-reported European Organisation for Research and Treatment of Cancer Quality of Life Questionaire-Core-15-Palliative Care questionnaire, assessing health-related quality of life. The data support the estimation of currently five quality of care indicators, ie, the proportions of 1) referred and eligible patients who were actually admitted to SPC, 2) patients who waited <10 days before admission to SPC, 3) patients who died from cancer and who obtained contact with SPC, 4) patients who were screened with European Organisation for Research and Treatment of Cancer Quality of Life Questionaire-Core-15-Palliative Care at admission to SPC, and 5) patients who were discussed at a multidisciplinary conference. Descriptive data In 2014, all 43 SPC units in Denmark reported their data to DPD, and all 9,434 cancer patients (100%) referred to SPC were registered in DPD. In total, 41,104 unique cancer patients were registered in DPD during the 5 years 2010–2014. Of those registered, 96% had cancer. Conclusion DPD is a national clinical quality database for SPC having clinically relevant variables and high data and patient completeness. PMID:27822111

  6. Campylobacter spp. - prevalence on pig livers and antimicrobial susceptibility.

    PubMed

    von Altrock, Alexandra; Hamedy, Ahmad; Merle, Roswitha; Waldmann, Karl-Heinz

    2013-04-01

    The objective of the study was to determine the prevalence of Campylobacter spp. on surfaces of slaughtered pig livers. Multilocus sequence typing (MLST) was performed to determine the sequence types (STs) of selected Campylobacter coli isolates. Additionally, C. coli and Campylobacter jejuni isolates were tested for antimicrobial susceptibility by the broth dilution method. The minimal inhibitory concentrations were determined for erythromycin, gentamicin, ampicillin, ampicillin/sulbactam, nalidixic acid, ciprofloxacin, tetracycline and trimethoprim/sulphamethoxazole. Samples were taken during the slaughtering process in a slaughterhouse in Lower Saxony, Germany. Altogether, 10% of 1500 surfaces of pig livers from 50 fattening herds was found to be Campylobacter positive, with C. coli as the predominant species (76%) followed by C. jejuni (21%). Resistance to erythromycin and tetracycline was higher in C. jejuni compared to C. coli, whereas C. coli were more resistant to quinolone compared to C. jejuni. Fluoroquinolone resistance is usually associated with cross-resistance to quinolone, but in the presented investigation C. coli as well as C. jejuni showed a higher resistance to ciprofloxacin (28.6% and 20.0%, respectively) than to nalidixic acid (9.5% and 0%, respectively). A high genetic diversity of the C. coli isolates was demonstrated by MLST. Differences in STs and antimicrobial resistance pattern indicate that the Campylobacter strains originated from the pig itself and not from the slaughterhouse. A comparison of the STs with those reported in the C. jejuni/coli PubMLST database showed an overlap of porcine and human isolates, indicating that C. coli isolates from pigs should be considered as potential sources of human infection.

  7. Effect of slaughter age and sex on the production output of South African Black ostriches.

    PubMed

    Brand, T S; Jordaan, J W; Bhiya, C S; Aucamp, B B

    2010-08-01

    1. The effects of different slaughter ages and sex on the yield and quality of economically important end-products of slaughtered ostriches was examined to determine the most economic slaughter age for growing/finishing ostriches. Two batches of 4- and 6-month-old ostriches were assigned to 10 treatment groups and fed ad libitum up to slaughter ages of 8·5, 10·5, 12·5, 14·5 and 16·5 months. Slaughter weight, cold carcase yield, skin surface area, dry skin grade, feather yield and feed intake of ostriches were measured for each age. 2. Cold carcase yields and total feather yields of males were higher than females but yields of other products were similar. 3. Slaughter weight, cold carcase yield, skin surface area, dry skin grade, feather yield and feed intake increased with age with significant differences between most age groups. Cold carcase weight increased by approximately 2·2 kg and skin surface area increased by 3·1 dm³ with each additional month of growth but the quality (grade) of skins and the proportion of first grade skins decreased with increasing age. This, together with an increase in feed intake associated with age to slaughtering should be taken into account when determining the optimal slaughter age. 4. The set of biological variables established in this study can be used to determine the most economical slaughter age under varying market conditions.

  8. Preweaning housing effects on behavior and physiological measures in pigs during the suckling and fattening periods.

    PubMed

    Chaloupková, H; Illmann, G; Neuhauserová, K; Tománek, M; Valis, L

    2007-07-01

    The effect of the preweaning housing system on the stress response of pigs before weaning and during fattening was studied in 33 litters of domestic pigs. Three preweaning housing systems were compared: barren crate (standard farrowing crate without straw), enriched crate (20% larger crate, with straw), and as a control, a farrowing pen (pen, 60% larger than the barren crate, with straw). At 25 d of age, pigs were tested with an isolation test and 1 d later with a human approach test (HumanT). Pigs were weaned at 28 d of age. At 3 and 6 mo of age, pigs were tested with an isolation-human approach test. The latency and frequency of squeal calls and locomotor activity were analyzed for all 3 tests, whereas physical contact with the human was also analyzed for the HumanT and isolation-human approach test. At 6 mo of age, the pigs were transported to a slaughterhouse. One day before transport, immediately after transport, and 1 h after transport, saliva samples were taken for cortisol analysis. The pH of the LM was also measured 45 min after slaughter. Preweaning housing system affected (P < 0.05) the probability of squeal vocalizations, the latency of locomotion, and the duration of locomotion during the HumanT. Pigs from the enriched pens vocalized less, had a longer latency to move, and performed less overall locomotion than pigs from the barren crates. Preweaning housing system did not affect behavior of fattening pigs. Cortisol concentrations before and after transport were not affected by preweaning housing system. An interaction of cortisol concentrations and housing systems was observed between the control sample and the sample taken immediately after transport in pigs from the barren crates (P < 0.05) compared with pigs from the enriched housing systems. Meat from pigs reared in the barren crate tended to have lower pH (P < 0.10) and that of pigs reared in enriched crates had lower pH (P < 0.05) than meat of pigs reared in enriched pens. No differences were

  9. Pre-slaughter, slaughter and post-slaughter defects of skins and hides at the Sheba Tannery and Leather Industry, Tigray region, northern Ethiopia.

    PubMed

    Kahsay, Tesfay; Negash, Guesh; Hagos, Yohannes; Hadush, Birhanu

    2015-08-21

    Skins and hides are perishable resources that can be damaged by parasitic diseases and human error, which result in downgrading or rejection. This study was conducted to identify defect types and to determine their prevalence in pickled sheep and wet blue goat skins and wet blue hides. Each selected skin or hide was examined for defects in natural light and the defects were graded according to established quality criteria in Ethiopian standard manuals. Major defects were captured by digital photography. The major pre-slaughter defects included scratches (64.2%), cockle (ekek) (32.8%), wounds or scars (12.6%), lesions from pox or lumpy skin disease (6.1%), poor substance (5%), branding marks (2.3%) and tick bites (1.5%). The presence of grain scratches in wet blue hides (76.3%) was significantly higher than in pickled sheep (67.2%) and wet blue goat (59.1%) skins. The major slaughter defects included flay cuts or scores, holes, poor pattern and vein marks, with a higher occurrence in wet blue goat skins (28.7%; P < 0.001) than in wet blue hides (22.8%) and pickled sheep skins (11.1%). The most prevalent postslaughter defects were grain cracks (14.9%), hide beetle damage (8%), damage caused by heat or putrefaction (3.7%) and machine-induced defects (0.5%). Grain cracks (27.04%) and hide beetle damage (13.9%) in wet blue goat skins were significantly more common than in wet blue hides and pickled sheep skins. These defects cause depreciation in the value of the hides and skins. Statistically significant (P < 0.001) higher rejection rates were recorded for wet blue hides (82.9%) than for pickled sheep skins (18.3%) and wet blue goat skins (8.5%). Improved animal health service delivery, effective disease control strategies and strong collaboration between stakeholders are suggested to enhance the quality of skins and hides.

  10. High prevalence of Toxoplasma gondii antibodies in domestic pigs in Oaxaca State, Mexico.

    PubMed

    Alvarado-Esquivel, C; Estrada-Malacón, M A; Reyes-Hernández, S O; Pérez-Ramírez, J A; Trujillo-López, J I; Villena, I; Dubey, J P

    2012-12-01

    Pigs are important in the epidemiology of toxoplasmosis in North America. Seroprevalence of Toxoplasma gondii infection in 525 domestic pigs (337 backyard raised, 188 farm raised) in Oaxaca State, Mexico was determined using the modified agglutination test (MAT, cutoff 1:25). Antibodies to T. gondii were found in 58 (17.2%) of the 337 backyard pigs with MAT titers of 1:25 in 10, 1:50 in 12, 1:100 in 18, 1:200 in 6, 1:400 in 6, 1:800 in 3, and 1:1,600 in 3. Seropositive pigs were found in 39 (37.9%) of 103 homes in all 7 municipalities surveyed. Seroprevalence of T. gondii infection in backyard pigs varied with age, gender, geographic region, climate, and altitude. High seroprevalence was found in pigs ≥ 9-mo-old (40%), in females (40%), in pigs from the Istmo region (33.3%), and in those raised in a tropical climate (65%). Seroprevalence was higher (24.5%) in pigs raised at 100-660 m above sea level than in those at 20-60 m of altitude (14.2%). With respect to farm pigs, only 1 (0.5%, MAT titer 1:100) of 188 pigs from 5 farms was positive for anti- T. gondii antibodies. The results affirm that the management system (outdoor vs. indoor system with biosecurity) is a key factor in the epidemiology of porcine toxoplasmosis. Because there is no national system of determining the T. gondii infection status at the time of slaughter, precautions should be taken while handling pig carcasses, and all pork should be cooked thoroughly before human consumption.

  11. Prevalence of hydatid cysts in slaughtered animals in Sirte, Libya.

    PubMed

    Kassem, Hamed H; Abdel-Kader, Abdel-Kader M; Nass, Sedigh Ahmed

    2013-04-01

    The prevalence of cystic echinococcosis was studied among the livestock slaughtered in abattoir of Sirte, Libya during the period July 2004 to May 2005. The overall infection rate of 4.9% in sheep, 2.4% in goats, 2.7% in camels and 15% in cattle were observed. The increase in prevalence with age of the animals was statistically significant in the four species. In female goats, examined infection was higher in the male. Liver had higher hydatid cysts than lungs in sheep, goat while infected lungs had higher in camel.

  12. Kosher slaughter paradigms: Evaluation of slaughterhouse inspection procedures.

    PubMed

    Bozzo, Giancarlo; Di Pinto, Angela; Bonerba, Elisabetta; Ceci, Edmondo; Mottola, Anna; Roma, Rocco; Capozza, Paolo; Samoilis, Giorgio; Tantillo, Giuseppina; Celano, Gaetano Vitale

    2017-06-01

    Slaughter by Jewish religious rite is the killing of an animal by cutting the trachea and oesophagus and major blood vessels using a very sharp blade. This operation is subject to strict rules laid down by religious authorities that characterize its sacredness. The aim of the study was to evaluate the specific criteria inherent in the Jewish religious rite, by analysing reject rates during the different phases. In this study, 52.4% of the carcasses failed to quality as Kosher, with 22.9% being rejected due to pulmonary lesions and only 3% for miscuts. The study also revealed legal vacuums in the field of labelling rules.

  13. Effects of pig age at market weight and magnesium supplementation through drinking water on pork quality.

    PubMed

    Frederick, B R; van Heugten, E; See, M T

    2006-06-01

    Thirty-two halothane-negative pigs (109 +/- 0.6 kg of BW) were used to determine the effect of pig age at marketing (and thus growth rate), and magnesium supplementation through drinking water, on pork quality. Two initial groups of 50 pigs that differed by 30 +/- 2 d of age were fed diets to meet or exceed nutrient requirements beginning at 28 kg of BW. Sixteen average, representative pigs were selected from each group to represent older, slow-growing pigs and younger, fast-growing pigs. For the duration of the study, pigs were individually penned, provided 2.7 kg of feed (0.12% Mg) daily, and allowed free access to water. After 7 d of adjustment, pigs were blocked by sex and BW and allotted to 0 or 900 mg of supplemental Mg/L as MgSO4 in drinking water for 2 d before slaughter. All 32 pigs were then transported (110 km) to a commercial abattoir on the same day and slaughtered 2.5 h after arrival. Longissimus and semimembranosus (SM) chops were packaged and stored to simulate display storage for fluid loss and Minolta color determinations at 0, 2, 4, 6, and 8 d. Two remaining sections of the LM were vacuum-packaged and stored at 4 degrees C for 25 or 50 d. Fast- (younger) and slow- (older) growing pigs differed by 27 +/- 0.3 d of age (153 and 180 +/- 0.3 d; P < 0.001) at similar BW (108 and 110 +/- 0.6 kg of BW; P = 0.13). Supplementation of Mg tended to increase plasma Mg concentration (24.1 vs. 21.8 +/- 0.8 ppm; P = 0.06) but did not affect Mg concentration in LM or SM. Fluid loss of displayed LM or SM, and purge loss, color, and oxidation of vacuum-packaged LM or SM were not affected by age or Mg (P > 0.10). Surface exudate of the SM from older pigs was lower than that of younger pigs (61 vs. 74 +/- 6 mg; P = 0.05) but was not different for the LM (P = 0.22). The LM from older pigs displayed for 4 and 8 d; P < 0.05) were less yellow (lower b*) than younger pigs. The SM from older pigs had lower lightness (L*) initially (47.9 vs. 49.5 +/- 0.4) and after 2 d (49

  14. Microbiological contamination in Three Large-Scale Pig Slaughterhouses in Northern Italy

    PubMed Central

    Di Ciccio, Pierluigi; Ossiprandi, Maria Cristina; Zanardi, Emanuela; Ghidini, Sergio; Belluzzi, Giancarlo; Vergara, Alberto; Ianieri, Adriana

    2016-01-01

    The aim of this survey was to obtain data on microbiological contamination of pig carcasses and environments in three large-scale Italian slaughterhouses (identified as A-B-C) located in Northern Italy. Each slaughterhouse was visited six times. Five carcasses and three different sites of the slaughterhouse (before and during slaughter) were sampled on each sampling day. A single pooled caecal sample was taken on each sampling day. A total of 90 carcasses, 108 environmental samples and 18 caecal samples were collected. Samples from pig carcasses and slaughterhouse environment were analyzed for total viable count (TVC), Enterobacteriaceae count (EBC) and Salmonella. The caecal contents were examined for Salmonella. Carcasses from slaughterhouse A presented the greatest TVC and EBC mean log value, whereas environmental samples collected during slaughter activities from slaughterhouse C showed the greatest TVC and EBC mean log value. As far as the environmental samples collected before slaughter activities are concerned, an average up to 6 log10 colony forming unit (CFU)/cm2 TVC in two slaughter plants (A and C) and 5 log10 CFU/cm2 TVC in one slaughter plant (B) was detected. Salmonella was recovered in two slaughterhouses (A and B). Four different Salmonella serotypes were detected in the positive samples (11). Within serotype S. Rissen and S. Typhimurium monophasic-variant isolates, two pulsed-field gel electrophoresis patterns were identified. The findings in this survey suggest that carcass contamination is influenced by the slaughterhouse plant and this could be a result of differences in line speed. The results of environmental sampling have not shown an association with the slaughterhouse plant. PMID:28058247

  15. Does meatiness of pigs depend on the level of gastro-intestinal parasites infection?

    PubMed

    Knecht, Damian; Popiołek, Marcin; Zaleśny, Grzegorz

    2011-05-01

    The aim of the present paper was to determine an influence of the presence and a level of intestine parasites infection on the quality of pork carcass expressed by the content of meat in carcass (meatiness) in pigs. The experimental part of the study was conducted on pigs farm produced in a closed cycle. The population in the study included 120 fattening pigs maintained in two keeping systems: group I--60 individuals kept on slatted floor, and group II--60 individuals kept on deep litter. All the experimental animals were treated in the same manner. The analysed fatteners were slaughtered in Meat Processing Plant when their body mass reached 110 kg, and the post-slaughter assessment was conducted according to the EUROP classification of pigs carcass using the Ultra-Fom 300 device. The study concerning the internal parasites were conducted basing on coproscopic quantitative McMaster method. As a results, the eggs of three nematode taxa were isolated and identified: Oesophagostomum spp., Ascaris suum and Strongyloides ransomi. Overall prevalence of infection of fatteners kept on litter was lower (25%±11.2) as compared to those kept on slatted floor (38.3%±12.6), however the differences were not statistically significant (χ(2)=2.465; df=1; P=0.116). The mean value of meatiness for pigs free from parasites was 53.68, while in the case of infected pigs the meatiness was statistically lower and was 52.12 (t=2.35; P=0.02). The analysed pigs were classified into three categories and conducted analysis of an influence of parasites on meatiness demonstrate the relationship that is statistically significant. The analysis of correlation between meatiness and an average number of helminth eggs also demonstrated the negative, statistically significant, relationship (F=5.52; P=0.020), i.e. in fatteners with higher EPG value the meatiness was lower.

  16. Comparison of neural histomorphology in tail tips from pigs docked using clippers or cautery iron.

    PubMed

    Kells, N J; Beausoleil, N J; Johnson, C B; Sutherland, M A; Morrison, R S; Roe, W

    2016-12-01

    Tail docking of pigs is commonly performed to reduce the incidence of unwanted tail-biting behaviour. Two docking methods are commonly used: blunt trauma cutting (i.e. using side clippers), or cutting and concurrent cauterisation using a hot cautery iron. A potential consequence of tail amputation is the development of neuromas at the docking site. Neuromas have been linked to neuropathic pain, which can influence the longer-term welfare of affected individuals. To determine whether method of tail docking influences the extent of neuroma formation, 75 pigs were allocated to one of three treatments at birth: tail docked using clippers; tail docked using cautery iron; tail left intact. Tail docking was performed at 2 days of age and pigs were kept under conventional conditions until slaughter at 21 weeks of age. Tails were removed following slaughter and subjected to histological examination. Nerve histomorphology was scored according to the following scale: 1=discrete well-organised nerve bundles; 2=moderate neural proliferation and disorganisation affecting more than half of the circumference of the tail; 3=marked neural proliferation to form almost continuous disorganised bundles or non-continuous enlarged bundles compressing the surrounding connective tissue. Scores of 2 or 3 indicated neuroma formation. Scores were higher in docked pigs than undocked pigs (P<0.001), but did not differ between pigs docked using clippers and those docked using cautery (P=0.23). The results indicate that tail docking using either clippers or cautery results in neuroma formation, thus having the potential to affect long-term pig welfare.

  17. Distribution of Mycobacterium avium Complex Isolates in Tissue Samples of Pigs Fed Peat Naturally Contaminated with Mycobacteria as a Supplement

    PubMed Central

    Matlova, Ludmila; Dvorska, Lenka; Ayele, Wuhib Yayo; Bartos, Milan; Amemori, Takashi; Pavlik, Ivo

    2005-01-01

    In early 1999, there was an increased incidence of tuberculous lesions in the lymph nodes of slaughtered pigs in the Czech Republic. In part 1 of this study, tuberculous lesions were detected in 140 (62%) tissue samples collected from pigs coming from 15 farms in 15 districts at routine veterinary meat inspections in abattoirs. Mycobacteria were isolated from 37 (16%) tissue samples: 34 Mycobacterium avium subsp. hominissuis isolates and three environmentally derived mycobacteria. In search of infection sources, M. avium subsp. hominissuis was isolated from 38 (79%) samples of peat used as a feed supplement. In part 2 of our study, the head, mesenteric, and inguinal lymph nodes of 117 randomly selected slaughtered pigs from one farm with young piglets fed peat as a supplement were investigated for mycobacterial infection. From 65 (56%) pigs, a total of 76 mycobacterial isolates were identified (56 M. avium subsp. hominissuis isolates, 5 M. avium subsp. avium isolates, 3 M. intracellulare isolates, and 12 environmentally derived mycobacterial isolates). IS1245 restriction fragment length polymorphism (RFLP) types with >20 bands of 45 distinct RFLP types were found in 49 M. avium subsp. hominissuis isolates from pigs (n = 31) and peat (n = 18). Identical RFLP types were found in only four pig isolates. Five randomly selected isolates from pigs and peat were subcultured to six independent clones or colonies. Among the IS1245 RFLP types of 30 clones, identical RFLP types obtained from pigs and peat were identified, which confirmed the hypothesis that peat contaminated with mycobacteria represents a significant source of mycobacterial infection for pigs. PMID:15750094

  18. Human brucellosis at a pig slaughterhouse.

    PubMed

    Escobar, Gabriela I; Jacob, Néstor R; López, Gustavo; Ayala, Sandra M; Whatmore, Adrian M; Lucero, Nidia E

    2013-12-01

    Seventeen workers in a pig slaughterhouse with signs and symptoms compatible with brucellosis were clinically examined at the outpatient service of different health institutions and studied by serological tests during the period 2005-2011. Eleven blood cultures were taken and six Brucella suis strains were isolated, three biovar 1 and three with atypical characteristics. In order to confirm that these cases had no common source, a variable number of tandem repeat (VNTR) analyses were performed on 5 of the 6 strains whose results showed substantial heterogeneity in the genotypes, thereby demonstrating that the immediate origin was not the same. Two hundred adult pigs admitted for slaughter at the plant were sampled by convenience and tested by buffered antigen plate test (BPAT), serum agglutination test (SAT) and 2-mercapto-ethanol test (MET). Seven of 62 males (11%) and 25/138 (18%) females tested positive. The study results contribute information on risk scenarios for packing plant workers and underscore the need to improve plant workers' education on appropriate containment measures and to actively screen animals for swine brucellosis.

  19. Transgenesis for pig models

    PubMed Central

    Yum, Soo-Young; Yoon, Ki-Young; Lee, Choong-Il; Lee, Byeong-Chun

    2016-01-01

    Animal models, particularly pigs, have come to play an important role in translational biomedical research. There have been many pig models with genetically modifications via somatic cell nuclear transfer (SCNT). However, because most transgenic pigs have been produced by random integration to date, the necessity for more exact gene-mutated models using recombinase based conditional gene expression like mice has been raised. Currently, advanced genome-editing technologies enable us to generate specific gene-deleted and -inserted pig models. In the future, the development of pig models with gene editing technologies could be a valuable resource for biomedical research. PMID:27030199

  20. Socio-economic, industrial and cultural parameters of pig-borne infections.

    PubMed

    Pappas, G

    2013-07-01

    The pork-processing industry has been possibly the fastest growing sector of the food industry in recent years. Specialization, genetic homogenization of the pig population, high density of the breeding population, reduced human-animal interactions, slaughter at a lower age and increased international trade of live animals and pork are parameters that affect, positively or negatively, the emergence of novel pig-borne pathogens, many of which are pig-specific, and many of which have significant zoonotic potential, as observed in recent outbreaks of Nipah virus and Streptococcus suis in Southeast Asia and China, respectively. Numerous other pathogens are transmitted to humans through direct contact with or consumption of pig products, and globalization trends in trade and human population movements have resulted in outbreaks of pig-borne diseases even in Muslim countries and in Israel, where pork consumption is religiously prohibited. The role of pigs as potential reservoirs of antibiotic-resistant pathogens or genes encoding resistance, and the role of feral pigs as a reservoir of zoonotic disease, are scientific fields in direct need of further research.

  1. Nature and Nationhood: Danish Perspectives

    ERIC Educational Resources Information Center

    Schnack, Karsten

    2009-01-01

    In this paper, I shall discuss Danish perspectives on nature, showing the interdependence of conceptions of "nature" and "nationhood" in the formations of a particular cultural community. Nature, thus construed, is never innocent of culture and cannot therefore simply be "restored" to some pristine, pre-lapsarian…

  2. Sheep carrying pathogenic Yersinia enterocolitica bioserotypes 2/O:9 and 5/O:3 in the feces at slaughter.

    PubMed

    Joutsen, Suvi; Eklund, Kirsi-Maria; Laukkanen-Ninios, Riikka; Stephan, Roger; Fredriksson-Ahomaa, Maria

    2016-12-25

    Yersinia enterocolitica is a heterogeneous species including non-pathogenic strains belonging to biotype 1A and pathogenic strains belonging to biotypes 1B and 2-5. Pathogenic strains of biotypes 2-4 carrying the ail virulence gene have frequently been isolated from domestic pigs at slaughter. In sheep, mostly non-pathogenic biotype 1A strains have been reported. In our study, the prevalence of ail-positive Y. enterocolitica was studied by PCR and culturing in 406 young sheep (<1year of age) and 139 older sheep at slaughter in Finland. When using PCR, the detection rate was 11% (45/406) in young sheep originating from 11 (18%) farms. Surprisingly, Y. enterocolitica belonging to bioserotypes 2/O:9 and 5/O:3, carrying both chromosomal and plasmid-borne virulence genes, were isolated from the fecal samples of 10 (2%) and 23 (4%) sheep, respectively. All isolates of bioserotypes 2/O:9 (19 isolates) and 5/O:3 (53 isolates) carried the chromosomal virulence genes ail, inv, ystA, and myfA, and almost all isolates (71/72) also carried the virulence genes virF and yadA located on the virulence plasmid. The isolates showed high susceptibility to tested antimicrobials and low genetic diversity by PFGE. Y. enterocolitica bioserotype 5/O:3 is a very rare bioserotype, and has earlier only sporadically been reported in European wildlife and in sheep in Australia and New Zealand. Bioserotype 2/O:9 is a common bioserotype found in humans with yersiniosis, and has sporadically been isolated in wild and domestic animals.

  3. Developing control points for halal slaughtering of poultry.

    PubMed

    Shahdan, I A; Regenstein, J M; Shahabuddin, A S M; Rahman, M T

    2016-07-01

    Halal (permissible or lawful) poultry meat production must meet industry, economic, and production needs, and government health requirements without compromising the Islamic religious requirements derived from the Qur'an and the Hadiths (the actions and sayings of the Prophet Muhammad, peace and blessings be upon him). Halal certification authorities may vary in their interpretation of these teachings, which leads to differences in halal slaughter requirements. The current study proposes 6 control points (CP) for halal poultry meat production based on the most commonly used halal production systems. CP 1 describes what is allowed and prohibited, such as blood and animal manure, and feed ingredients for halal poultry meat production. CP 2 describes the requirements for humane handling during lairage. CP 3 describes different methods for immobilizing poultry, when immobilization is used, such as water bath stunning. CP 4 describes the importance of intention, details of the halal slaughter, and the equipment permitted. CP 5 and CP 6 describe the requirements after the neck cut has been made such as the time needed before the carcasses can enter the scalding tank, and the potential for meat adulteration with fecal residues and blood. It is important to note that the proposed halal CP program is presented as a starting point for any individual halal certifying body to improve its practices.

  4. Quantification of contamination levels and particular risk of Salmonella spp. in pigs in slaughterhouses in Chiang Mai and Lamphun provinces, Thailand.

    PubMed

    Tadee, Pakpoom; Boonkhot, Phacharaporn; Patchanee, Prapas

    2014-11-01

    Salmonella spp. is one of the important foodborne pathogens, and the slaughtering process is recognized as a potential point of contamination and the spread of the pathogens. The three objectives of this study are first, to quantify the contamination levels of Salmonella spp. in pig skins and carcasses, second, to evaluate the outcomes from different pig supply sources and different practices at three critical steps (scalding, splitting, and washing) for Salmonella spp. contamination, and third, to assess risk of Salmonella spp. contamination in pork products after slaughtering level. The study was performed in three representative slaughterhouses in Chiang Mai and Lamphun provinces, Thailand. Investigation conducted from May 2013 through October 2013 found the overall prevalence and contamination levels mean to be 11.85% and 0.34 MPN/cm2, respectively. There was no statistically significant in Salmonella spp. prevalence and contamination levels detected with different patterns at the slaughterhouses which were supplied pigs from either co-operative or integrated farms. Factors found to reduce Salmonella spp. loads on carcasses included good practices, e.g., regular changing of water in the scalding tank after each batch and the use of chlorine in the washing step. Risk of Salmonella spp. contamination of pork products at the final stage of slaughtering was nearly 10%. Good practices and proper hygiene measures should be applied to minimize the risk of Salmonella spp. exposure in the slaughtering line, which can reduce the contamination pressure downstream at retail shops as well as for end consumers.

  5. Evaluation of recombinant Bhlp29.7 as an ELISA antigen for detecting pig herds with swine dysentery.

    PubMed

    La, Tom; Phillips, Nyree D; Hampson, David J

    2009-01-01

    Swine dysentery (SD) results from infection of the porcine large intestine with the anaerobic intestinal spirochaete Brachyspira hyodysenteriae. Diagnosis of SD traditionally has relied on detecting the spirochaete in the faeces of acutely affected pigs. To date simple and reliable serological assays that can be applied as a diagnostic tool at the herd level have not been available. In the current study a recombinant histidine tagged 29.7 kDa lipoprotein of B. hyodysenteriae (His6-Bhlp29.7) was used as an ELISA plate-coating antigen. Sera (n=1121) from slaughter-aged pigs on 19 farms were tested in this ELISA. Following optimization of the ELISA conditions using hyperimmune control sera, a set of 464 sera from slaughter-aged pigs from five herds where SD did not occur was tested. From these results a suitable cut-off value for herd negativity was defined as the mean optical density reading plus three standard deviations. Testing of 337 pig sera from six farms with SD then showed that the sensitivity of the test at the herd level was 100%, with all six farms having one or more serum samples exceeding the cut-off value for negativity. Finally, 320 sera from eight herds suspected of having SD were examined. Four of these herds were shown to have pigs with titres consistent with SD. The true health status of the other four herds that were serologically negative could not be confirmed. In conclusion, when used on sets of 40 sera from slaughter-aged pigs the His6-Bhlp29.7 ELISA as established proved to be a useful adjunct to the diagnosis of SD at the herd level.

  6. Predicting fat, lean and the weights of primal cuts for growing pigs of different genotypes and sexes using computed tomography.

    PubMed

    Carabús, A; Sainz, R D; Oltjen, J W; Gispert, M; Font-i-Furnols, M

    2015-03-01

    The aim of the present study was to find single equations to predict the amounts of fat, lean, and the weights of the primal cuts (ham, loin, belly, and shoulder) as well as ham composition of pigs from 30 to 120 kg BW of different genotypes (GEN; Exp. 1) and sexual conditions (SEX; Exp. 2). Two types of regression equations, taking into account different work situations, were developed: 1) research applications, using computed tomography (CT) parameters, and 2) potential on-farm applications, which could be obtained using easily accessible equipment. Two data sets were used: Exp. 1 included 90 gilts from 3 different GEN: 30 Duroc × (Landrace × Large White), 30 Pietrain × (Landrace × Large White), and 30 Landrace × Large White, and Exp. 2 included 92 Pietrain × (Landrace × Duroc) pigs of different SEX: 24 each of females, entire males, castrated males, and 20 immunocastrated males. Pigs were fully CT scanned in vivo at 30, 70, 100, and 120 kg BW. A subsample of pigs of each GEN ( = 5) or SEX ( = 4) were slaughtered at 30, 70, and 100 kg BW, and all remaining pigs were slaughtered after weighing and scanning at 120 kg BW. For all the slaughtered pigs, the 4 main cuts were fully (GEN) or partially dissected (SEX). CT images were analyzed and used to predict the lean and fat contents as well as the weights of the primal cuts and the composition of the ham. Total amounts of fat and lean for both populations were predicted with high levels of accuracy ( = 0.994 and 0.993, respectively) and proportions of random error for GEN and SEX effects (0.998 and 0.946 for the fat and 0.997 and 0.836 for the lean predictions, respectively). Moreover, the composition of ham (fat, lean, and bone) was very well predicted with high proportions (> 80%) of random error for GEN and SEX effect using CT and potential on-farm predictors.

  7. Increased Foraging in Outdoor Organic Pig Production—Modeling Environmental Consequences

    PubMed Central

    Jakobsen, Malene; Preda, Teodora; Kongsted, Anne Grete; Hermansen, John Erik

    2015-01-01

    Consumers’ motivations for buying organic products include a wish of acquiring healthy, environmentally friendly products from production systems that also ensure a high level of animal welfare. However, the current Danish organic pig production faces important challenges regarding environmental impact of the system. High ammonia emissions arise from outdoor concrete areas with growing-finishing pigs and sows on pasture possess an increased risk of nitrogen (N) leaching. Direct foraging in the range area is suggested as a way to improve the nutrient efficiency at farm level and to support a more natural behavior of the pig. Thus, by modeling, we investigated the environmental consequences of two alternative scenarios with growing-finishing pigs foraging in the range area and different levels of crops available for foraging; grass-clover (lowest integration of forage) or a combination of lucerne, grass-clover and Jerusalem artichokes (highest integration of forage). It was possible to have growing-finishing pigs on free-range without increasing N leaching compared to the current practice. The alternative system with lucerne, grass-clover and Jerusalem artichokes showed the lowest carbon footprint with 3.12 CO2 eq kg−1 live weight pig compared to the current Danish pasture based system with 3.69 kg CO2 eq kg−1 live weight pig. Due to positive impact on soil carbon sequestration, the second alternative system based on grass-clover  showed a similar carbon foot print compared to current practice with 3.68 kg CO2 eq kg−1 live weight pig. It is concluded that in practice there is room for development of organic pig production systems where direct foraging plays a central role. PMID:28231226

  8. 9 CFR 71.21 - Tissue and blood testing at slaughter.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Tissue and blood testing at slaughter... GENERAL PROVISIONS § 71.21 Tissue and blood testing at slaughter. (a) Any person moving livestock or... in accordance with paragraph (b) of this section 9 within their facility for blood and tissue...

  9. 9 CFR 71.21 - Tissue and blood testing at slaughter.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Tissue and blood testing at slaughter... GENERAL PROVISIONS § 71.21 Tissue and blood testing at slaughter. (a) Any person moving livestock or... in accordance with paragraph (b) of this section 9 within their facility for blood and tissue...

  10. 9 CFR 313.30 - Electrical; stunning or slaughtering with electric current.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... with electric current. 313.30 Section 313.30 Animals and Animal Products FOOD SAFETY AND INSPECTION... Electrical; stunning or slaughtering with electric current. The slaughtering of swine, sheep, calves, cattle, and goats with the use of electric current and the handling in connection therewith, in...

  11. 9 CFR 146.4 - General provisions for all participating flocks and slaughter plants.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false General provisions for all participating flocks and slaughter plants. 146.4 Section 146.4 Animals and Animal Products ANIMAL AND PLANT... and slaughter plants. (a) Records that establish the identity of products handled shall be...

  12. 9 CFR 146.6 - Specific provisions for participating slaughter plants.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Specific provisions for participating slaughter plants. 146.6 Section 146.6 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE... General Provisions § 146.6 Specific provisions for participating slaughter plants. (a) Only...

  13. 9 CFR 146.4 - General provisions for all participating flocks and slaughter plants.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false General provisions for all participating flocks and slaughter plants. 146.4 Section 146.4 Animals and Animal Products ANIMAL AND PLANT... and slaughter plants. (a) Records that establish the identity of products handled shall be...

  14. 9 CFR 146.4 - General provisions for all participating flocks and slaughter plants.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false General provisions for all participating flocks and slaughter plants. 146.4 Section 146.4 Animals and Animal Products ANIMAL AND PLANT... and slaughter plants. (a) Records that establish the identity of products handled shall be...

  15. 9 CFR 93.518 - Swine from Canada for immediate slaughter.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Swine from Canada for immediate...; REQUIREMENTS FOR MEANS OF CONVEYANCE AND SHIPPING CONTAINERS Swine Canada 7 § 93.518 Swine from Canada for immediate slaughter. Swine imported from Canada for immediate slaughter shall be consigned from the port...

  16. Comparison of Campylobacter populations isolated from a free-range broiler flock before and after slaughter.

    PubMed

    Colles, Frances M; McCarthy, Noel D; Sheppard, Samuel K; Layton, Ruth; Maiden, Martin C J

    2010-02-28

    Relatively little is known about the Campylobacter genotypes colonizing extensively reared broiler flocks and their survival through the slaughter process, despite the increasing demand for free-range and organic products by the consumer. Campylobacter isolates from a free-range boiler flock, sampled before and after slaughter, were genotyped by MLST (multilocus sequence typing) and sequence analysis of the flaA short variable region (SVR). The Campylobacter genotypes isolated before and after slaughter were diverse, with up to five sequence types (STs) (seven-locus allelic profiles resulting from MLST) identified per live bird, up to eight STs identified per carcass and 31 STs identified in all. The majority (72.0%) of isolates sampled from carcasses post-slaughter were indistinguishable from those isolated from the live flock before slaughter by ST and flaA SVR type, however, sampling 'on-farm' failed to capture all of the diversity seen post-slaughter. There were statistically significant increases in the genetic diversity of Campylobacter (p=0.005) and the proportion of C. coli (p=0.002), with some evidence for differential survival of genotypes contaminating the end product. C. coli genotypes isolated after slaughter were more similar to those from free-range and organic meat products sampled nationally, than from the live flock sampled previously. This study demonstrated the utility of MLST in detecting genetic diversity before and after the slaughter process.

  17. 9 CFR 71.21 - Tissue and blood testing at slaughter.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Tissue and blood testing at slaughter... GENERAL PROVISIONS § 71.21 Tissue and blood testing at slaughter. (a) Any person moving livestock or... in accordance with paragraph (b) of this section 9 within their facility for blood and tissue...

  18. 9 CFR 71.21 - Tissue and blood testing at slaughter.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Tissue and blood testing at slaughter... GENERAL PROVISIONS § 71.21 Tissue and blood testing at slaughter. (a) Any person moving livestock or... this section 9 within their facility for blood and tissue sample collection; 9 FSIS also has...

  19. 9 CFR 71.21 - Tissue and blood testing at slaughter.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Tissue and blood testing at slaughter... GENERAL PROVISIONS § 71.21 Tissue and blood testing at slaughter. (a) Any person moving livestock or... in accordance with paragraph (b) of this section 9 within their facility for blood and tissue...

  20. Defeathering of broiler carcasses subjected to delayed scalding 1, 2, 4, and 8 hours after slaughter

    Technology Transfer Automated Retrieval System (TEKTRAN)

    With implementation of farm slaughter, scalding and defeathering could be delayed for a minimum of 2 to 4 h. This research evaluated the potential for delaying scalding and defeathering up to 8 h after slaughter. Following 12 h feed withdrawal broilers were cooped and transported to the pilot plan...

  1. 9 CFR 93.316 - Horses from Canada for immediate slaughter.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Horses from Canada for immediate...; REQUIREMENTS FOR MEANS OF CONVEYANCE AND SHIPPING CONTAINERS Horses Canada 16 § 93.316 Horses from Canada for immediate slaughter. Horses imported from Canada for immediate slaughter shall be consigned from the port...

  2. 9 CFR 93.316 - Horses from Canada for immediate slaughter.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Horses from Canada for immediate...; REQUIREMENTS FOR MEANS OF CONVEYANCE AND SHIPPING CONTAINERS Horses Canada 16 § 93.316 Horses from Canada for immediate slaughter. Horses imported from Canada for immediate slaughter shall be consigned from the port...

  3. 9 CFR 93.316 - Horses from Canada for immediate slaughter.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Horses from Canada for immediate...; REQUIREMENTS FOR MEANS OF CONVEYANCE AND SHIPPING CONTAINERS Horses Canada 16 § 93.316 Horses from Canada for immediate slaughter. Horses imported from Canada for immediate slaughter shall be consigned from the port...

  4. 9 CFR 93.316 - Horses from Canada for immediate slaughter.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Horses from Canada for immediate...; REQUIREMENTS FOR MEANS OF CONVEYANCE AND SHIPPING CONTAINERS Horses Canada 16 § 93.316 Horses from Canada for immediate slaughter. Horses imported from Canada for immediate slaughter shall be consigned from the port...

  5. 9 CFR 93.316 - Horses from Canada for immediate slaughter.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Horses from Canada for immediate...; REQUIREMENTS FOR MEANS OF CONVEYANCE AND SHIPPING CONTAINERS Horses Canada 16 § 93.316 Horses from Canada for immediate slaughter. Horses imported from Canada for immediate slaughter shall be consigned from the port...

  6. Severe seizures in pigs naturally infected with Taenia solium in Tanzania.

    PubMed

    Trevisan, Chiara; Mkupasi, Ernatus M; Ngowi, Helena A; Forkman, Björn; Johansen, Maria V

    2016-04-15

    Neurocysticercosis (NCC) caused by Taenia solium is a serious neurological disease. In humans neurological symptoms have been thoroughly studied and documented, however, there is limited information on clinical signs in pigs infected with T. solium cysticerci. Among the scientific community, it is in fact believed that pigs with NCC rarely show neurological signs. The aim of this study was to describe clinical manifestations associated with NCC in pigs and correlate the manifestations to the number and distribution of cysticerci in brains of naturally infected pigs in Tanzania. Sixteen infected and 15 non-infected control pigs were observed for 14 days during daylight hours, and subsequently videotaped for another 14 consecutive days using close circuit television cameras. All occurrences of abnormal behaviour (trembling, twitching, mouth and ear paralysis, ataxia, dribbling, salivating, eye blinking, walking in circles) were recorded. At the end of the recording period, pigs were slaughtered and their brains dissected, cysticerci counted and locations noted. During the recording period, two infected pigs were observed having seizures. Some of the observed autonomic signs during a seizure were chewing motions with foamy salivation and ear stiffening. Motor signs included tonic muscle contractions followed by a sudden diminution in all muscle function leading to collapse of the animal. Stereotypic walking in circles was observed on several occasions. At dissection, both pigs had a high number of brain cysticerci (241 and 247 cysticerci). The two pigs with seizures were also older (36 months) compared to the others (18.3 months, ± 8.2 standard deviation). Results of this study have shown that pigs with NCC can develop clinical signs and suffer from seizures like humans with symptomatic NCC. Results of this study could potentially open up a new experimental pathway to explore the aetiology of neurological symptoms in humans with NCC associated epilepsy.

  7. Severe seizures in pigs naturally infected with Taenia solium in Tanzania

    PubMed Central

    Trevisan, Chiara; Mkupasi, Ernatus M; Ngowi, Helena A; Forkman, Björn; Johansen, Maria V

    2016-01-01

    Neurocysticercosis (NCC) caused by Taenia solium is a serious neurological disease. In humans neurological symptoms have been thoroughly studied and documented, however, there is limited information on clinical signs in pigs infected with T. solium cysticerci. Among the scientific community, it is in fact believed that pigs with NCC rarely show neurological signs. The aim of this study was to describe clinical manifestations associated with NCC in pigs and correlate the manifestations to the number and distribution of cysticerci in brains of naturally infected pigs in Tanzania. Sixteen infected and 15 non-infected control pigs were observed for 14 days during daylight hours, and subsequently videotaped for another 14 consecutive days using close circuit television cameras. All occurrences of abnormal behaviour (trembling, twitching, mouth and ear paralysis, ataxia, dribbling, salivating, eye blinking, walking in circles) were recorded. At the end of the recording period, pigs were slaughtered and their brains dissected, cysticerci counted and locations noted. During the recording period, two infected pigs were observed having seizures. Some of the observed autonomic signs during a seizure were chewing motions with foamy salivation and ear stiffening. Motor signs included tonic muscle contractions followed by a sudden diminution in all muscle function leading to collapse of the animal. Stereotypic walking in circles was observed on several occasions. At dissection, both pigs had a high number of brain cysticerci (241 and 247 cysticerci). The two pigs with seizures were also older (36 months) compared to the others (18.3 months, ± 8.2 standard deviation). Results of this study have shown that pigs with NCC can develop clinical signs and suffer from seizures like humans with symptomatic NCC. Results of this study could potentially open up a new experimental pathway to explore the aetiology of neurological symptoms in humans with NCC associated epilepsy. PMID:26995723

  8. Simultaneous occurrence of Salmonella enterica, Campylobacter spp. and Yersinia enterocolitica along the pork production chain from farm to meat processing in five conventional fattening pig herds in Lower Saxony.

    PubMed

    Niemann, Jana-Kristin; Alter, Thomas; Gölz, Greta; Tietze, Erhard; Fruth, Angelika; Rabsch, Wolfgang; von Münchhausen, Christiane; Merle, Roswitha; Kreienbrock, Lothar

    2016-01-01

    The objectives of this study were to gather data on the occurrence of Salmonella (S.) enterica, Campylobacter spp. and Yersinia (Y.) enterocolitica along the pork production chain and to further analyze detected Salmonella isolates by additionally applying MLVA (multiple-locus variable-number tandem repeat analysis). In total, 336 samples were collected at primary production, slaughter and meat processing from five conventional fattening pig farms and one common slaughterhouse. At farm level, S. enterica, Campylobacter spp. and Y. enterocolitica were detected in 19.4%, 38.9% and 11.1% of pooled fecal samples of fattening pigs. At slaughter, more than two-thirds of examined carcasses, 24% of carcass surfaces samples and about 60% of cecal content samples were positive for at least one of the examined pathogens. An amount of 4% of meat samples were positive for non-human pathogenic Y. enterocolitica. Identical MLVA patterns of Salmonella isolates from farm- and associated slaughterhouse samples demonstrated transmission across both production stages. Other MLVA patterns found at slaughter indicated possible colonization of pigs during transport or lairage and/or cross-contamination during slaughter. Identical MLVA patterns from risk tissues and the nearby carcass surface evidenced a direct contamination of carcasses as well. Overall, our data showed wide distribution ranges for all three examined pathogens within the pig production chain and underline the need for appropriate intervention strategies at pre- and postharvest.

  9. Effects of scalding and dehairing of pig carcasses at abattoirs on the visibility of welfare-related lesions.

    PubMed

    Carroll, G A; Boyle, L A; Teixeira, D L; van Staaveren, N; Hanlon, A; O'Connell, N E

    2016-03-01

    There is increasing interest in developing abattoir-based measures to assist in determining the welfare status of pigs. The primary aim of this study was to determine the most appropriate place on the slaughter line to conduct assessments of welfare-related lesions, namely apparent aggression-related skin lesions (hereafter referred to as 'skin lesions'), loin bruising and apparent tail biting damage. The study also lent itself to an assessment of the prevalence of these lesions, and the extent to which they were linked with production variables. Finishing pigs processed at two abattoirs on the Island of Ireland (n=1950 in abattoir A, and n=1939 in abattoir B) were used. Data were collected over 6 days in each abattoir in July 2014. Lesion scoring took place at two points on the slaughter line: (1) at exsanguination (slaughter stage 1 (SS1)), and (2) following scalding and dehairing of carcasses (slaughter stage 2 (SS2)). At both points, each carcass was assigned a skin and tail lesion score ranging from 0 (lesion absent) to 3 or 4 (severe lesions), respectively. Loin bruising was recorded as present or absent. Differences in the percentage of pigs with observable lesions of each type were compared between SS1 and SS2 using McNemar/McNemar-Bowker tests. The associations between each lesion type, and both cold carcass weight and condemnations, were examined at batch level using Pearson's correlations. Batch was defined as the group of animals with a particular farm identification code on a given day. The overall percentage of pigs with a visible skin lesion (i.e. score>0) decreased between SS1 and SS2 (P<0.001). However, the percentage of pigs with a severe skin lesion increased numerically from SS1 to SS2. The percentage of pigs with a visible tail lesion and with loin bruising also increased between SS1 and SS2 (P<0.001). There was a positive correlation between the percentage of carcasses that were partially condemned, and the percentage of pigs with skin lesions

  10. Topographic distribution of gastritis in heavy pigs investigated by a geographic information system approach.

    PubMed

    Pascotto, Ernesto; Capraro, Diego; Tomè, Paolo; Spanghero, Mauro

    2016-05-31

    The aim of this paper was to determine the topographic distribution of gastritis lesions in pigs through an open source geographic information system (GIS) software analysis. The stomachs of 146 Italian heavy pigs were collected at slaughter and subjected to macroscopic pathological examination of the internal mucosa. A total of 623 lesions were either classified as hyperplastic or follicular (97%) with the remaining minority of lesions categorised as atrophic and simple. The hyperplastic gastritis lesions had an average surface of 77.8 cm2 and were mainly located in an oval shaped area of the fundus region of the stomach near the Curvatura ventriculi major. The follicular gastritis lesions had generally a smaller surface (40.3 cm2) and were concentrated in two distinct small areas of the pyloric region. The GIS analysis provided the opportunity to produce useful maps showing the distribution and characteristics of gastritis in pigs.

  11. The use of acute phase proteins for monitoring animal health and welfare in the pig production chain: the validation of an immunochromatographic method for the detection of elevated levels of pig-MAP.

    PubMed

    Piñeiro, Matilde; Morales, Joaquín; Vizcaíno, Elena; Murillo, José Alberto; Klauke, Thorsten; Petersen, Brigitte; Piñeiro, Carlos

    2013-11-01

    The serum concentration of acute phase proteins (APPs) increases in the presence of disease or stress, which makes APPs notable parameters for the global assessment of animal health and welfare. A rapid, immunochromatographic test (ICT) for the detection of elevated levels of pig Major Acute-phase Protein (pig-MAP), one of the main APPs in pigs, was evaluated in more than 1400 pig serum samples obtained from commercial farms. The ICT showed a good performance with a relative sensitivity (Sn) and specificity (Sp) of 94 and 97%, respectively, for a threshold of 1.5mg/mL (comparison with ELISA). Differences in the pig-MAP levels and the number of positive samples with the ICT were observed within the season of sampling, farms, and age groups at one farm, according to the presence of disease or lesions. The ICT was also evaluated in blood samples obtained at slaughter in association with the carcase inspection. The results from this study indicate that the ICT may be used for the evaluation of groups of pigs, after analysing one sub-sample of these pigs, and might be a useful tool in routine health and welfare monitoring programmes aimed to improve the quality of pig production.

  12. Impact of dietary betaine and conjugated linoleic acid on insulin sensitivity, protein and fat metabolism of obese pigs.

    PubMed

    Fernández-Fígares, I; Lachica, M; Martín, A; Nieto, R; González-Valero, L; Rodríguez-López, J M; Aguilera, J F

    2012-07-01

    To determine possible mechanisms of action that might explain the nutrient partitioning effect of betaine and conjugated linoleic acid (CLA) in Iberian pigs and to address potential adverse effects, twenty gilts were restrictively fed from 20 to 50 kg BW Control, 0.5% betaine, 1% CLA or 0.5% betaine + 1% CLA diets. Serum hormones and metabolites profile were determined at 30 kg BW and an oral glucose test was performed before slaughter. Pigs were slaughtered at 50 kg BW and livers were obtained for chemical and histological analysis. Decreased serum urea in pigs fed betaine and betaine + CLA diets (11%; P = 0.0001) indicated a more efficient N utilization. The increase in serum triacylglycerol (58% and 28%, respectively; P = 0.0098) indicated that CLA and betaine + CLA could have reduced adipose tissue triacylglycerol synthesis from preformed fatty acids. Serum glucose, low-density lipoprotein (LDL) cholesterol and non-esterified fatty acids were unaffected. CLA and betaine + CLA altered serum lipids profile, although liver of pigs fed CLA diet presented no histopathological changes and triglyceride content was not different from Control pigs. Compared with controls, serum growth hormone decreased (20% to 23%; P = 0.0209) for all treatments. Although serum insulin increased in CLA, and especially in betaine + CLA pigs (28% and 83%; P = 0.0001), indices of insulin resistance were unaffected. In conclusion, CLA, and especially betaine + CLA, induced changes in biochemical parameters and hormones that may partially explain a nutrient partitioning effect in young pigs. Nevertheless, they exhibited weak, although detrimental, effects on blood lipids. Moreover, although livers were chemically and histologically normal, pigs fed CLA diet challenged with a glucose load had higher serum glucose than controls.

  13. Comparison of different commercial serological tests for the detection of Toxoplasma gondii antibodies in serum of naturally exposed pigs.

    PubMed

    Steinparzer, R; Reisp, K; Grünberger, B; Köfer, J; Schmoll, F; Sattler, T

    2015-03-01

    Toxoplasma gondii is the aetiological agent of the zoonotic disease toxoplasmosis and transmitted among other ways by chemically and physically untreated, that is, raw pork to humans. The detection of Toxoplasma gondii is impossible by currently practiced meat inspection, but serological tests can be used to detect Toxoplasma gondii antibodies in pig herds and can consequently be helpful to identify potentially contaminated pork. Therefore, appropriate serological tests are required. In this study, serum samples of 1368 naturally exposed slaughter pigs from 73 Austrian farms were collected. Serum samples of at least 16 slaughter pigs per farm were tested. The prevalence of Toxoplasma gondii antibodies in serum was measured by a commercial available modified agglutination test (MAT) and compared to three different commercial available enzyme-linked immunosorbent assays (ELISA). The MAT detected 6.5%, ELISA I 6.7%, ELISA II 4.8% and ELISA III 4.3% of the pigs as Toxoplasma gondii antibody positive. The agreement, according to the kappa coefficient (κ), was substantial between the MAT and ELISA I (κ = 0.62), II (κ = 0.64) and III (κ = 0.67). A better agreement was determined between ELISA I and II (κ = 0.715), ELISA I and III (κ = 0.747) and ELISA II and III (κ = 0.865). At least one pig per farm was detected Toxoplasma gondii antibody positive in 17 (23.3%) farms by the MAT, 26 (35.6%) farms by ELISA I, 16 (21.9%) farms by ELISA II and 11 (15.1%) farms by ELISA III. Pig farms with a high number of Toxoplasma gondii antibody-positive pigs or high antibody titres were identified by all of the four used serological tests. Concerning the occurrence of Toxoplasma gondii antibodies in Austrian pig farms, a monitoring and surveillance programme would be reasonable to find high-risk farms.

  14. 9 CFR 381.67 - Young chicken and squab slaughter inspection rate maximums under traditional inspection procedure.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Young chicken and squab slaughter... INSPECTION REGULATIONS Operating Procedures § 381.67 Young chicken and squab slaughter inspection rate... slaughter line configurations are specified in the following table. These maximum rates will not be...

  15. 9 CFR 71.14 - Slaughter of poultry or other animals to prevent spread of disease; ascertainment of value and...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Slaughter of poultry or other animals... TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS GENERAL PROVISIONS § 71.14 Slaughter of poultry... to slaughter any diseased or exposed animals, including poultry, and the purchase of such...

  16. 9 CFR 71.14 - Slaughter of poultry or other animals to prevent spread of disease; ascertainment of value and...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Slaughter of poultry or other animals... TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS GENERAL PROVISIONS § 71.14 Slaughter of poultry... to slaughter any diseased or exposed animals, including poultry, and the purchase of such...

  17. 9 CFR 71.14 - Slaughter of poultry or other animals to prevent spread of disease; ascertainment of value and...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Slaughter of poultry or other animals... TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS GENERAL PROVISIONS § 71.14 Slaughter of poultry... to slaughter any diseased or exposed animals, including poultry, and the purchase of such...

  18. 9 CFR 71.14 - Slaughter of poultry or other animals to prevent spread of disease; ascertainment of value and...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Slaughter of poultry or other animals... TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS GENERAL PROVISIONS § 71.14 Slaughter of poultry... to slaughter any diseased or exposed animals, including poultry, and the purchase of such...

  19. 9 CFR 71.14 - Slaughter of poultry or other animals to prevent spread of disease; ascertainment of value and...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Slaughter of poultry or other animals... TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS GENERAL PROVISIONS § 71.14 Slaughter of poultry... to slaughter any diseased or exposed animals, including poultry, and the purchase of such...

  20. Feather retention force in broiler carcasses slaughtered and held up to 8 hours postmortem prior to scalding

    Technology Transfer Automated Retrieval System (TEKTRAN)

    One factor that could impact the feasibility of commercial on-farm slaughter of broilers is the time delay from on-farm slaughter to scalding and defeathering in the commercial plant that could be 4 h or more. This experiment evaluated feather retention force (FRF) in broilers that were slaughtered ...

  1. Pathophysiology of free-bullet slaughter of horses and ponies.

    PubMed

    Gibson, Troy J; Bedford, Elisabeth M; Chancellor, Natalie M; Limon, Georgina

    2015-10-01

    Forty-six equines were observed during routine commercial slaughter in an abattoir. The animals were shot once with a .22 calibre long rifle with hollow point rounds. Indicators of sensibility/insensibility were evaluated immediately after the shot (prior to exsanguination) and the resulting pathophysiology of free-bullet injury was assessed. All animals were rendered immediately insensible, with only one pony showing signs of a shallow depth of concussion, with an intermittently positive palpebral reflex but no other signs of brainstem function. All animals (100%) had some degree of damage to the structures of the brainstem or lobes of the cerebrums, while 41 (89%) had damage to the thalamus/hypothalamus. The bullet in one pony missed the brain but still caused mild damage to the thalamus, midbrain, pons and cerebellum, this animal had no signs of sensibility. The findings confirm that free-bullet shooting is an effective dispatch method for horses and ponies.

  2. Effects of a "step-up" ractopamine feeding program, sex, and social rank on growth performance, hoof lesions, and Enterobacteriaceae shedding in finishing pigs.

    PubMed

    Poletto, R; Rostagno, M H; Richert, B T; Marchant-Forde, J N

    2009-01-01

    Increasing concern for animal well-being and food safety has stimulated the investigation of feed additives such as ractopamine (RAC), a beta-agonist widely used to improve production performance of finishing pigs. The objective of this study was to determine effects of RAC feeding, delivered as a "step-up" program (5 mg/kg for 2 wk followed by 10 mg/kg for 2 wk), on growth performance, Enterobacteriaceae shedding, including Salmonella, and hoof lesions, also taking into account sex and social rank of pigs. A total of 64 barrows and gilts (balanced by treatment and sex) were assigned to pens of 4 (by sex) as either control (CTL) or RAC treatment. Social ranks (dominant, intermediate, and subordinate) of pigs in each pen were determined by behavioral observation during 48 h post-mixing. Fecal samples were collected once per week for 5 wk. At slaughter, the 32 dominant and subordinate barrows and gilts (16/sex) were examined for hoof lesions, and luminal contents from ileum, cecum, and rectum were collected. Pigs fed RAC had increased growth performance (P < 0.05) with social rank of animals affecting overall ADG (P < 0.05). Gilts gained more backfat than barrows when comparing to baseline values at both 10th and last ribs (P < 0.05), whereas loin eye area increased at a similar rate for both barrows and gilts (P > 0.10). No significant effect of RAC feeding was found on backfat or loin eye area (P > 0.10). At slaughter, RAC-fed pigs had greater BW (P < 0.05). Despite the positive effects of RAC feeding on growth performance, pigs fed the compound had a greater frequency of front and rear hoof lesions as did barrows and dominant individuals (P < 0.05). Detectable concentrations of Salmonella shedding were not identified at any time during the experiment. Enterobacteriaceae shedding concentrations from RAC-fed pigs peaked at the first week of feeding and progressively decreased until slaughter. At slaughter, rectal and cecal Enterobacteriaceae concentrations were less

  3. Dietary-induced changes of muscle growth rate in pigs: effects on in vivo and postmortem muscle proteolysis and meat quality.

    PubMed

    Kristensen, L; Therkildsen, M; Riis, B; Sørensen, M T; Oksbjerg, N; Purslow, P P; Ertbjerg, P

    2002-11-01

    The effects of various growth rates in pigs induced by four different feeding strategies on the activity of the calpain system and on postmortem (PM) muscle proteolysis and tenderness development were studied. An increased growth rate may be caused by an increased protein turnover, which results in up-regulated levels of proteolytic enzymes in vivo that, in turn, possibly will affect PM tenderness development. It can be hypothesized that increased proteolytic activity pre-slaughter will increase the PM tenderization rate. From postnatal d 28 to d 90 (phase 1) the pigs were divided into two groups, given either ad libitum (A) or restricted (R, 60% of ad libitum) access to feed. The two groups were then divided into two subgroups, given either restricted or ad libitum access to feed from d 91 to slaughter at d 165 (phase 2). Measurements of the activity of mu-calpain, m-calpain, and calpastatin; concentrations of total collagen and the percent of soluble collagen; and RNA, DNA, and elongation factor-2 where made at slaugther. Myofibrillar fragmentation index (MFI) was determined at slaughter and 24 h PM. Warner-Braztler shear force was determined 1 d and 4 d PM. Pigs fed restricted diets in phase 1 and fed ad libitum in phase 2 (RA pigs) had increased growth rates in the last phase compared to pigs fed ad libitum during both phase 1 and phase 2 (AA pigs). The increased growth rate (compensatory growth) was followed by an increased proteolytic potential (mu-calpain:calpastatin ratio), increased MFI values, and higher tenderization rates. There was a positive correlation between the activities of m-calpain and growth rates (r = 0.35, P = 0.03), and between RNA levels and growth rates (r = 0.43, P = 0.006). The proposed hypothesis is largely supported by the results. The activities of both mu- and m-calpain at slaughter were highest in fast-growing pigs. The calpain activity was highest in RA pigs, which in turn also had the fastest growth rates prior tslaughter among

  4. Taenia solium: the complex interactions, of biological, social, geographical and commercial factors, involved in the transmission dynamics of pig cysticercosis in highly endemic areas.

    PubMed

    Morales, J; Martínez, J J; Garcia-Castella, J; Peña, N; Maza, V; Villalobos, N; Aluja, A S; Fleury, A; Fragoso, G; Larralde, C; Sciutto, E

    2006-03-01

    If a programme for the control of pig cysticercosis is to be effective it has to be based on good data on the local epidemiology of Taenia solium. In 2002-2003, in a cross-sectional study of pig cysticercosis in the Mexican state of Morelos, 1747 pigs that had been born and reared in rural areas of the state were checked for T. solium infection by tongue inspection. The prevalence of cysticercosis in the pigs was found to vary from 0% to 30% according to the municipality from which the pigs came. Although prevalence appeared to be unaffected by the socio-economic status of the municipality, it was relatively high in areas that lacked latrines, and in pigs that were castrated, pregnant and/or of the native (rather than an imported) breed. The results of questionnaire-based interviews with pig owners revealed that most (64.5%) of the rural pigs, whether infected or not, are slaughtered and consumed within the locality where they were reared. The other pigs are sold at low prices to organised traffickers who take the uninspected pigs to neighbouring urban areas for sale. The observed complexity in the factors affecting the transmission of T. solium to the pigs of Morelos state calls for an intervention strategy of matching complexity, initially targeted at those villages with the highest prevalences of pig cysticercosis. The road transport of pigs needs to be better regulated, and the vaccination and genetic improvement of the rural pigs, and delaying the castration of the boars, should all be considered.

  5. An evaluation of the use of cottonseed cake in the diet of growing pigs.

    PubMed

    Fombad, R B; Bryant, M J

    2004-04-01

    An experiment was conducted to determine the effects of including cottonseed cake in rations for weaned growing pigs. Thirty-two Landrace x Large White pigs, weighing 20-24 kg, were included in four blocks formed on the basis of initial weight within sex in an otherwise completely randomized block design. The pigs were killed when they reached a live weight of 75.0 +/- 2.0 kg and the half carcases were analysed into cuts and the weights of the organs were recorded. An estimate of the productivity of the pigs on each diet was calculated. Cottonseed cake reduced the voluntary feed intake (p < 0.001) and live weight gains p < 0.001) and increased the heart, kidney and liver weights (p < 0.01). The pigs on the soya bean-based control diet took the shortest time to reach slaughter weight. The result was probably in part due to lysine deficiency and in part to the effect of free gossypol. It was found that it is at present cost-effective to include cottonseed cake in pig weaner-grower diets up to 300 g/kg in Cameroon.

  6. The marketing of cysticercotic pigs in the Sierra of Peru. The Cysticercosis Working Group in Peru.

    PubMed Central

    1993-01-01

    In Peru pork supplied through regulated slaughterhouses is primarily restricted to the large cities on the coast. Approximately 65% of the pork consumed in the country is obtained through informal channels that are not inspected or supervised. The pathways via which pigs are commercialized were studied in Huancayo, a city in the Peruvian Sierra where cysticercosis is endemic. Official purchase, slaughter, and market records were reviewed. Also, direct surveys and participant observation were carried out at two local live pig markets and at two informal meat markets. Pigs were not processed in local slaughterhouses; instead, they were butchered informally. The proportion of cysticercotic pigs detected by tongue examination ranged from 14% to 25% of the total sold. Since cysticercotic pigs and pork are sold through informal markets, surveys of abattoirs and meat markets are not a reliable way to monitor the prevalence of porcine cysticercosis in Peru. We estimate that 48% of the pork traded informally and 23% of the total pork consumed in Huancayo is derived from pigs that are infected with cysticercosis. PMID:8490986

  7. The marketing of cysticercotic pigs in the Sierra of Peru. The Cysticercosis Working Group in Peru.

    PubMed

    1993-01-01

    In Peru pork supplied through regulated slaughterhouses is primarily restricted to the large cities on the coast. Approximately 65% of the pork consumed in the country is obtained through informal channels that are not inspected or supervised. The pathways via which pigs are commercialized were studied in Huancayo, a city in the Peruvian Sierra where cysticercosis is endemic. Official purchase, slaughter, and market records were reviewed. Also, direct surveys and participant observation were carried out at two local live pig markets and at two informal meat markets. Pigs were not processed in local slaughterhouses; instead, they were butchered informally. The proportion of cysticercotic pigs detected by tongue examination ranged from 14% to 25% of the total sold. Since cysticercotic pigs and pork are sold through informal markets, surveys of abattoirs and meat markets are not a reliable way to monitor the prevalence of porcine cysticercosis in Peru. We estimate that 48% of the pork traded informally and 23% of the total pork consumed in Huancayo is derived from pigs that are infected with cysticercosis.

  8. Increased Foraging in Outdoor Organic Pig Production-Modeling Environmental Consequences.

    PubMed

    Jakobsen, Malene; Preda, Teodora; Kongsted, Anne Grete; Hermansen, John Erik

    2015-11-02

    Consumers' motivations for buying organic products include a wish of acquiring healthy, environmentally friendly products from production systems that also ensure a high level of animal welfare. However, the current Danish organic pig production faces important challenges regarding environmental impact of the system. High ammonia emissions arise from outdoor concrete areas with growing pigs and sows on pasture possess an increased risk of nitrogen (N) leaching. Direct foraging in the range area is suggested as a way to improve the nutrient efficiency at farm level and to support a more natural behavior of the pig. Thus, by modeling, we investigated the environmental consequences of two alternative scenarios with growing pigs foraging in the range area and different levels of crops available for foraging-grass-clover or a combination of Jerusalem artichokes and lucerne. It was possible to have growing pigs on free-range without increasing N leaching compared to the current practice. The alternative system with Jerusalem artichokes and lucerne (high integration of forage) showed the lowest carbon foot print with 3.12 CO₂ eq kg(-1) live weight pig compared to the current Danish pasture based system with 3.69 kg CO₂ eq kg(-1) live weight pig. Due to positive impact on soil carbon sequestration, the second alternative system based on grass-clover (low integration of forage) showed a similar carbon foot print compared to current practice with 3.68 kg CO₂ eq kg(-1) live weight pig. It is concluded that in practice there is room for development of organic farming systems where direct foraging plays a central role.

  9. Assessment of Welfare Issues During Traditional Slaughter of Goats in Pretoria, South Africa.

    PubMed

    Qekwana, Daniel N; McCrindle, Cheryl M E; Oguttu, James W; Grace, Delia; Cenci-Goga, Beniamino T

    2017-01-01

    Goats are traditionally slaughtered to celebrate marriages and births, venerate ancestors, address personal problems, or perform a ritual during funerals. The objective of this study was to assess nonhuman animal welfare issues associated with the traditional slaughter of goats in and around Pretoria, South Africa. Participatory research methods were used to interview 105 respondents. Four of those interviewed were visited to observe the slaughter process. The most common method of transport was a vehicle (47%), followed by transport on foot (30%). The distance traveled (68%) was usually less than 10 km, and in all cases, it was less than 50 km. The most common (57%) method of restraining goats during transport was tying all 4 legs together. During slaughter, assistants held the head and legs of the goat (55%). Prior to slaughter, the majority of goats were tied under a tree (66%). In total, 97% of the goats were slaughtered within 24 hr, and no stunning was performed. In this study, animal welfare problems were widespread. Research should be undertaken to find practical ways to address animal welfare issues during traditional slaughter.

  10. Pathological manifestations observed in dead-on-arrival broilers at a Danish abattoir.

    PubMed

    Lund, V P; Kyvsgaard, N C; Christensen, J P; Bisgaard, M

    2013-01-01

    1. The mortality of broilers during pre-slaughter handling, including harvesting and transport, is an issue of increasing public concern which has led to the adoption of Council Directive EC/43/2007 implementing abattoir surveillance regarding the number of dead-on-arrival (DOA) broilers. 2. Pathological lesions and causes of death of DOA broilers at a Danish abattoir were investigated in a cross-sectional study comprising 300 DOA broilers (25 broilers from each of 12 randomly selected flocks). Major pathological manifestations of DOA broilers included severe pulmonary congestion (51.5%), lung congestion in combination with trauma (12.5%), trauma (10.2%), nephropathy accompanied by dehydration and/or discolouration (8.8%), morbus cordis (2.0%), septicaemia (1.7%) and suspected septicaemia (1.0%). Lung congestion accompanied by circulatory disturbances in other tissues was suggested to be due to suffocation. 3. Analyses of pathological diagnoses revealed that DOA broilers can be divided into two main categories, lung congestion and trauma, based on the chronicity of the lesions, both of which are primarily related to management and handling procedures. Most DOA broilers examined (74.2%) were estimated to have died as a consequence of events during pre-slaughter handling underlining the importance of increased focus on handling-related factors to reduce DOA rate.

  11. Sensory characteristics and carcass traits of boars, barrows, and gilts fed high- or adequate-protein diets and slaughtered at 100 or 110 kilograms.

    PubMed

    Nold, R A; Romans, J R; Costello, W J; Henson, J A; Libal, G W

    1997-10-01

    The objective of this study was to determine consumer reaction to boar (BO), barrow (BA), and gilt (G) meat from pigs grown and finished on high- (HI) and low- (LO) protein diets and slaughtered at 100 and 110 kg BW. Within each of two trials, 54 BO, BA, and G were allotted within sexes to HI or LO protein sequences for growing and finishing: 19 and 17% (BOHI), 18 and 16% (BOLO), 17 and 15% (GHI), 16 and 14% (GLO), 15 and 13% (BAHI), and 14 and 12% (BALO). Backfat skatole and salivary gland 16-androstene concentrations were measured from samples taken at slaughter. Longissimus (LM) and semitendinosus (ST) chops from 24 pigs (with equal representation across diet and sex groups) were evaluated by trained panelists for tenderness, juiciness, and off-flavor. Consumer panelists evaluated acceptability of LM chops. In the 100-kg trial, HI diets improved (P < .05) carcass leanness in BO and BA but not in G. In both trials, BO were leaner (P < .05) than G, and both were leaner (P < .05) than BA. Skatole and 16-androstene concentrations were similar (P > .05) among sexes in both trials. In the 100-kg trial, trained panelists found BOLO chops had more (P < .05) off-flavor. In the 110-kg trial, all BO had more off-flavor (P < .05) than BAHI, BALO, and GHI but were similar (P > .05) to GLO. In both trials, BA chops were more tender (P < .05) than G and BO chops and LM chops had less off-flavor (P < .05) than ST chops. In the 110-kg trial, skatole was correlated (r = .28, P < .001) to off-flavor. A relationship may exist among diet, skatole deposition, and off-flavor. Untrained consumers reported all chops were equally acceptable (P > .05).

  12. Antibiotic Resistance in Escherichia coli from Pigs in Organic and Conventional Farming in Four European Countries

    PubMed Central

    Nygaard Jensen, Annette; Kerouanton, Annaelle; Cibin, Veronica; Barco, Lisa; Denis, Martine; Aabo, Sören

    2016-01-01

    Organic pig production differs in many ways from conventional production of pigs, e.g., in antibiotic use, herd structure, feeding regimes, access to outdoor areas and space allowance per pig. This study investigated if these differences result in a lower occurrence of antibiotic resistance in organic slaughter pigs in Denmark, France, Italy and Sweden. Samples were taken from the colon content and/or faeces and minimum inhibitory concentrations (MIC) of ten antibiotics were determined in isolates of Escherichia coli. In addition, the proportion of tetracycline (TET) resistant E. coli in colon content and/or faeces from individual pigs was determined. In all four countries the percentage resistance to ampicillin, streptomycin, sulphonamides or trimethoprim was significantly lower in E. coli from organic pigs. In France and Italy, the percentage of isolates resistant to chloramphenicol, ciprofloxacin, nalidixic acid or gentamicin was also significantly lower in the E. coli from organic pigs. Resistance to cefotaxime, was not found in any country. The percentage of E. coli isolates resistant to TET as well as the proportion of TET-resistant E. coli was significantly lower in organic than in conventional pigs, except in Sweden where TET-resistance was equally low in both production types. There were also differences between countries within production type in the percentage resistance to individual antibiotics as well as the proportion of TET-resistant E. coli with lower median proportions in Sweden and Denmark compared to France and Italy. The study shows that in each of the four countries resistance in intestinal E. coli was less common in organic than in conventional pigs, but that there were also large differences in resistance between countries within each production type, indicating that both country- and production-specific factors influence the occurrence of resistance. PMID:27362262

  13. Developmental changes of carcass composition, meat quality and organs in the Jinhua pig and Landrace.

    PubMed

    Miao, Z-G; Wang, L-J; Xu, Z-R; Huang, J-F; Wang, Y-R

    2009-03-01

    The present study was aimed to compare the developmental changes of carcass composition, meat quality characteristics and organ weight in pigs of different breeds. Six pigs (sex balance) of each breed were slaughtered at 35, 80 and 125 days of age, respectively. The carcass was chilled and the left carcass side was dissected into bone, lean meat, fat and skin; additionally, organ weight and meat quality parameters were observed. Carcasses of the Jinhua pig were lighter (P < 0.001), contained less lean meat percentage (P < 0.01) and more carcass fat percentage (P < 0.05) than did carcasses of the Landrace. L*-values were lower in Jinhua pigs than in Landrace at 125 days of age (P < 0.05), but the Jinhua pig had higher a*-values compared with Landrace at the age of 80 days (P < 0.01) and 125 days (P < 0.01), respectively. In addition, Jinhua pigs showed lower colour scores (P < 0.05), higher intramuscular fat (IMF) percentage (P < 0.05), less marbling scores (P < 0.05) and lower drip loss (P < 0.05) than Landrace. For organ weight, Jinhua pigs had higher relative heart weight at the age of 80 days (P < 0.05) and 125 days (P < 0.001), and higher relative liver weight at 125 days of age (P < 0.01) than that of Landrace. In addition, the relative kidney weight was heavier (P < 0.001) in the Jinhua pig than in the Landrace during the whole experiment. These results indicated that developmental changes of carcass composition, meat quality parameters and organ weight displayed breed differences. Jinhua pigs were fatter than Landrace but the former had better quality characteristics in the meat.

  14. Antibiotic Resistance in Escherichia coli from Pigs in Organic and Conventional Farming in Four European Countries.

    PubMed

    Österberg, Julia; Wingstrand, Anne; Nygaard Jensen, Annette; Kerouanton, Annaelle; Cibin, Veronica; Barco, Lisa; Denis, Martine; Aabo, Sören; Bengtsson, Björn

    2016-01-01

    Organic pig production differs in many ways from conventional production of pigs, e.g., in antibiotic use, herd structure, feeding regimes, access to outdoor areas and space allowance per pig. This study investigated if these differences result in a lower occurrence of antibiotic resistance in organic slaughter pigs in Denmark, France, Italy and Sweden. Samples were taken from the colon content and/or faeces and minimum inhibitory concentrations (MIC) of ten antibiotics were determined in isolates of Escherichia coli. In addition, the proportion of tetracycline (TET) resistant E. coli in colon content and/or faeces from individual pigs was determined. In all four countries the percentage resistance to ampicillin, streptomycin, sulphonamides or trimethoprim was significantly lower in E. coli from organic pigs. In France and Italy, the percentage of isolates resistant to chloramphenicol, ciprofloxacin, nalidixic acid or gentamicin was also significantly lower in the E. coli from organic pigs. Resistance to cefotaxime, was not found in any country. The percentage of E. coli isolates resistant to TET as well as the proportion of TET-resistant E. coli was significantly lower in organic than in conventional pigs, except in Sweden where TET-resistance was equally low in both production types. There were also differences between countries within production type in the percentage resistance to individual antibiotics as well as the proportion of TET-resistant E. coli with lower median proportions in Sweden and Denmark compared to France and Italy. The study shows that in each of the four countries resistance in intestinal E. coli was less common in organic than in conventional pigs, but that there were also large differences in resistance between countries within each production type, indicating that both country- and production-specific factors influence the occurrence of resistance.

  15. Identification of risk factors associated with poor lifetime growth performance in pigs.

    PubMed

    Douglas, S L; Edwards, S A; Sutcliffe, E; Knap, P W; Kyriazakis, I

    2013-09-01

    During the production period from birth to slaughter there are some pigs that grow markedly slower, despite conditions that seem to support the growth of their contemporaries. This reduction in growth inevitably leads to weight variation within a group, causes difficulties with management, and results in system inefficiencies. By understanding the factors that contribute to poor growth, the performance of these slow growing pigs might be improved, thereby decreasing the overall variability at slaughter. The aim of this paper was to analyze the factors associated with poor growth performance in pigs from birth to slaughter, determine the effect of piglet birth weight (BiW) and weaning weight (WW) on lifetime growth, and investigate the capacity of small piglets to compensate for any BW deficit. Two industry databases, with individual data for approximately 40,000 and 90,000 pigs, respectively, and containing BW profiles and relevant variables, were analyzed. Body weight at birth, weaning, intermediate, and finishing stages were available as well as sex, month of birth, litter size information (number born alive and total born including still born), sow parity number, and length of gestation. Absolute and relative growth rates, based on adjusted BW for age, were calculated for each time interval and 3 types of analysis were performed: a logistic regression, a continuous linear plateau model, and a weight category analysis. For both datasets poor absolute and relative growth from birth to final BW was associated with low BiW (P < 0.001), low WW (P < 0.001), sex (P < 0.001), breed code (P < 0.001), and month of birth (P < 0.001). The linear plateau model suggested that the relationship between BiW and lifetime growth was not linear beyond 1.91 (database 1) or 1.84 (database 2) kg; the same applied to the relationship between WW at 21 d and final BW (FW) growth, which was not linear beyond 7.53 kg. Finally, the weight category analysis revealed that piglets with the

  16. Bayesian estimation of true between-herd and within-herd prevalence of Salmonella in Danish veal calves.

    PubMed

    Nielsen, T D; Nielsen, L R; Toft, N

    2011-07-01

    Specialised veal producers that purchase and raise calves from several dairy herds are potentially at high risk of delivering Salmonella-infected animals to slaughter. However, the true prevalence of Salmonella infected veal producing herds and the prevalence of infected calves delivered to slaughter from infected herds are unknown in Denmark. Due to uncertainties about test sensitivity and specificity, these prevalences are not straightforward to assess. The objective of this study was to estimate the within-herd- and between-herd prevalence of Salmonella in veal calves delivered for slaughter to abattoirs in Denmark. Furthermore, it was investigated to which extent the estimates differed between a setup using both serological tests and faecal culture, compared to just serological tests, and whether the applied sampling scheme in the national surveillance programme in Denmark was sufficient to establish high posterior estimates of freedom from infection in individual herds. We used Bayesian analysis to avoid bias as a result of fixed test validity estimates. Serological test results from 753 animals and faecal culture from 1233 animals from 68 randomly selected Danish veal producing herds that delivered more than 100 calves to slaughter per year were used to estimate the prevalences and estimates of freedom from Salmonella. Serological test results of 7726 animals from 185 herds were used to compare the difference in prevalence estimates between serology alone vs. faecal culture combined with serology. We estimated that 34-57% of specialised veal producing herds were infected with Salmonella. Within the infected herds, 21-49% of the animals were infected. Few herds obtained high posterior estimates for the probability of freedom from infection given the collected data, with only six of 68 herds obtaining posterior probability of being infected less than 10%. Furthermore, this study indicated that serology is sufficiently sensitive and specific to be used for

  17. Bleeding Efficiency, Microbiological Quality and Oxidative Stability of Meat from Goats Subjected to Slaughter without Stunning in Comparison with Different Methods of Pre-Slaughter Electrical Stunning

    PubMed Central

    Sabow, Azad Behnan; Zulkifli, Idrus; Goh, Yong Meng; Ab Kadir, Mohd Zainal Abidin; Kaka, Ubedullah; Imlan, Jurhamid Columbres; Abubakar, Ahmed Abubakar; Adeyemi, Kazeem Dauda; Sazili, Awis Qurni

    2016-01-01

    The influence of pre-slaughter electrical stunning techniques and slaughter without stunning on bleeding efficiency and shelf life of chevon during a 14 d postmortem aging were assessed. Thirty two Boer crossbred bucks were randomly assigned to four slaughtering techniques viz slaughter without stunning (SWS), low frequency head-only electrical stunning (LFHO; 1 A for 3 s at a frequency of 50 Hz), low frequency head-to-back electrical stunning (LFHB; 1 A for 3 s at a frequency of 50 Hz) and high frequency head-to-back electrical stunning (HFHB; 1 A for 3 s at a frequency of 850 Hz). The SWS, LFHO and HFHB goats had higher (p<0.05) blood loss and lower residual hemoglobin in muscle compared to LFHB. The LFHB meat had higher (p<0.05) TBARS value than other treatments on d 7 and 14 d postmortem. Slaughtering methods had no effect on protein oxidation. Higher bacterial counts were observed in LFHB meat compared to those from SWS, LFHO and HFHB after 3 d postmortem. Results indicate that the low bleed-out in LFHB lowered the lipid oxidative stability and microbiological quality of chevon during aging. PMID:27035716

  18. Pig in the Middle.

    ERIC Educational Resources Information Center

    Mills, Sophie

    2000-01-01

    Explores themes relating to human transition as they appear in "Charlotte's Web" and four other stories using pigs as a subject. Discusses the motifs common to all these texts that recur in the film "Babe." Considers how the cycle of life and death is ceaseless, and pigs symbolize the necessary transitions that people must all…

  19. Why are most EU pigs tail docked? Economic and ethical analysis of four pig housing and management scenarios in the light of EU legislation and animal welfare outcomes.

    PubMed

    D'Eath, R B; Niemi, J K; Vosough Ahmadi, B; Rutherford, K M D; Ison, S H; Turner, S P; Anker, H T; Jensen, T; Busch, M E; Jensen, K K; Lawrence, A B; Sandøe, P

    2016-04-01

    To limit tail biting incidence, most pig producers in Europe tail dock their piglets. This is despite EU Council Directive 2008/120/EC banning routine tail docking and allowing it only as a last resort. The paper aims to understand what it takes to fulfil the intentions of the Directive by examining economic results of four management and housing scenarios, and by discussing their consequences for animal welfare in the light of legal and ethical considerations. The four scenarios compared are: 'Standard Docked', a conventional housing scenario with tail docking meeting the recommendations for Danish production (0.7 m2/pig); 'Standard Undocked', which is the same as 'Standard Docked' but with no tail docking, 'Efficient Undocked' and 'Enhanced Undocked', which have increased solid floor area (0.9 and 1.0 m2/pig, respectively) provision of loose manipulable materials (100 and 200 g/straw per pig per day) and no tail docking. A decision tree model based on data from Danish and Finnish pig production suggests that Standard Docked provides the highest economic gross margin with the least tail biting. Given our assumptions, Enhanced Undocked is the least economic, although Efficient Undocked is better economically and both result in a lower incidence of tail biting than Standard Undocked but higher than Standard Docked. For a pig, being bitten is worse for welfare (repeated pain, risk of infections) than being docked, but to compare welfare consequences at a farm level means considering the number of affected pigs. Because of the high levels of biting in Standard Undocked, it has on average inferior welfare to Standard Docked, whereas the comparison of Standard Docked and Enhanced (or Efficient) Undocked is more difficult. In Enhanced (or Efficient) Undocked, more pigs than in Standard Docked suffer from being tail bitten, whereas all the pigs avoid the acute pain of docking endured by the pigs in Standard Docked. We illustrate and discuss this ethical balance using

  20. Cysticercosis in the pig.

    PubMed

    de Aluja, A S

    2008-01-01

    Taenia solium cysticercosis is still an important parasitosis in rural pigs in many developing countries, México among them. The main causes for the persistence of this condition are lack of hygiene in the rural communities, lack of education of the animal owners, lack of control in the trade of pigs and their meat and lack of conscientious meat inspection. The pig production systems in the marginated areas of Mexico are briefly mentioned and it is stressed that among the important reasons for the persistence of the reproductive cycle of Taenia solium is the fact that appropriate toilet facilities in village dwellings are not mandatory. The diagnostic methods of cysticercosis in the living pigs and in their meat are discussed and the degenerative stages of the larvae as well as methods to test their viability are explained. The treatment of infected pigs and their meat is discussed. Recommendations for control programmes are given.

  1. Growth performance, carcass characteristics, meat quality, and tissue histology of growing pigs fed crude glycerin-supplemented diets.

    PubMed

    Lammers, P J; Kerr, B J; Weber, T E; Bregendahl, K; Lonergan, S M; Prusa, K J; Ahn, D U; Stoffregen, W C; Dozier, W A; Honeyman, M S

    2008-11-01

    The effects of dietary crude glycerin on growth performance, carcass characteristics, meat quality indices, and tissue histology in growing pigs were determined in a 138-d feeding trial. Crude glycerin utilized in the trial contained 84.51% glycerin, 11.95% water, 2.91% sodium chloride, and 0.32% methanol. Eight days postweaning, 96 pigs (48 barrows and 48 gilts, average BW of 7.9 +/- 0.4 kg) were allotted to 24 pens (4 pigs/pen), with sex and BW balanced at the start of the experiment. Dietary treatments were 0, 5, and 10% crude glycerin inclusion in corn-soybean meal-based diets and were randomly assigned to pens. Diets were offered ad libitum in meal form and formulated to be equal in ME, sodium, chloride, and Lys, with other AA balanced on an ideal AA basis. Pigs and feeders were weighed every other week to determine ADG, ADFI, and G:F. At the end of the trial, all pigs were scanned using real-time ultrasound and subsequently slaughtered at a commercial abattoir. Blood samples were collected pretransport and at the time of slaughter for plasma metabolite analysis. In addition, kidney, liver, and eye tissues were collected for subsequent examination for lesions characteristic of methanol toxicity. After an overnight chilling of the carcass, loins were removed for meat quality, sensory evaluation, and fatty acid profile analysis. Pig growth, feed intake, and G:F were not affected by dietary treatment. Dietary treatment did not affect 10th-rib backfat, LM area, percent fat free lean, meat quality, or sensory evaluation. Loin ultimate pH was increased (P = 0.06) in pigs fed the 5 and 10% crude glycerin compared with pigs fed no crude glycerin (5.65 and 5.65 versus 5.57, respectively). Fatty acid profile of the LM was slightly changed by diet with the LM from pigs fed 10% crude glycerin having less linoleic acid (P < 0.01) and more eicosapentaenoic acid (P = 0.02) than pigs fed the 0 or 5% crude glycerin diets. Dietary treatment did not affect blood metabolites or

  2. Reducing abortion: the Danish experience.

    PubMed

    Risor, H

    1989-01-01

    In 1987, 20,830 legal abortions were performed in Denmark. 2,845 involved women below the age of 20, and 532 involved women terminating pregnancy after the 12th week. Danish law permits all of its female citizens to have an abortion free-of-charge before the 12th week of pregnancy. After the 12th week, the abortion must be applied for through a committee of 3 members, and all counties in Denmark have a committee. It is felt in Denmark that a woman has a right to an abortion if she decides to have one. It she makes that choice, doctors and nurses are supportive. Since 1970, sex education has been mandatory in Danish schools. Teachers often collaborate closely with school doctors and nurses in this education. All counties are required to have at least 1 clinic that provides contraceptive counselling. It was recently found that the lowest number of pregnancies among teenaged girls was found in a county in Jutland where all 9th grade students visit the county clinic to learn about contraceptives, pregnancy, and abortion. Within 1 year after Copenhagen had adopted this practice, the number of abortions among teenagers declined by 20%. One fourth of all pharmacies also collaborate with schools to promote sex education, instructing students about contraceptives and pregnancy tests. The Danish Family Planning Association has produced a film on abortion, and plans to produce videos on abortion for use in schools. The organization also holds training programs for health care personnel on contraception, pregnancy, and abortion. By means of the practices described above, it is hoped that the number of abortions and unwanted pregnancies in Denmark will be reduced.

  3. The Danish Hip Arthroplasty Register

    PubMed Central

    Gundtoft, Per Hviid; Varnum, Claus; Pedersen, Alma Becic; Overgaard, Søren

    2016-01-01

    Aim of database The aim of the Danish Hip Arthroplasty Register (DHR) is to continuously monitor and improve the quality of treatment of primary and revision total hip arthroplasty (THA) in Denmark. Study population The DHR is a Danish nationwide arthroplasty register established in January 1995. All Danish orthopedic departments – both public and private – report to the register, and registration is compulsory. Main variables The main variables in the register include civil registration number, indication for primary and revision surgery, operation date and side, and postoperative complications. Completeness of primary and revision surgery is evaluated annually and validation of a number of variables has been carried out. Descriptive data A total of 139,525 primary THAs and 22,118 revisions have been registered in the DHR between January 1, 1995 and December 31, 2014. Since 1995, completeness of procedure registration has been high, being 97.8% and 92.0% in 2014 for primary THAs and revisions, respectively. Several risk factors, such as comorbidity, age, specific primary diagnosis and fixation types for failure of primary THAs, and postoperative complications, have been identified through the DHR. Approximately 9,000 primary THAs and 1,500 revisions are reported to the register annually. Conclusion The DHR is important for monitoring and improvement of treatment with THA and is a valuable tool for research in THA surgery due to the high quality of prospective collected data with long-term follow-up and high completeness. The register can be used for population-based epidemiology studies of THA surgery and can be linked to a range of other national databases. PMID:27822092

  4. Epidemiologic and economic evaluation of risk-based meat inspection for bovine cysticercosis in Danish cattle.

    PubMed

    Calvo-Artavia, F F; Nielsen, L R; Alban, L

    2013-03-01

    Under the current EU meat inspection regulation, every single carcase from all bovines above 6 weeks of age has to be examined for bovine cysticercosis (BC). This is time-consuming, costly, and is of limited value in countries with low prevalence. The aim of this study was to develop a stochastic simulation model for analysis of tentative risk-based meat inspection systems for BC in Danish cattle with regard to system sensitivity (SSSe), specificity and potential monetary benefits compared to the current system, which has an estimated SSSe of 15%. The relevant risk factors used to construct three alternative scenario trees were identified from previous Danish risk factor studies (1) gender, (2) grazing and (3) access to risky water sources. Thus, females, animals that had been grazing or animals with access to risky water sources were considered high-risk and would be subjected to invasive inspection at meat inspection. All animals in the low-risk groups (i.e. males, non-grazing or no access to risky water sources, respectively) would be subjected to visual inspection only. It was assumed that half of the cattle were slaughtered in abattoirs that would be able to reorganise the work at the slaughterline, allowing them to do with one meat inspector less. All abattoirs would gain on the price of sold uncut beef from the masseter muscles from visually inspected cattle. Under these assumptions, using gender and grazing were preferable due to them having SSSe only slightly lower than the current system, and highest effectiveness ratios, but they had a lower net economic effect (NEE) than the scenario using risky water sources. Using gender to differentiate high and low-risk groups was judged preferable over grazing due to feasibility, because the information is readily available at the slaughter line. The exact total NEE for the cattle sector depends on how many and which of the abattoirs that would be able to reorganise the work at the slaughter line to save money on

  5. 9 CFR 146.6 - Specific provisions for participating slaughter plants.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... upland game bird, commercial waterfowl, meat-type chicken, and meat-type turkey slaughter plants that are... participate in the Plan. (b) To participate in the Plan, meat-type chicken, meat-type turkey, and...

  6. 9 CFR 146.6 - Specific provisions for participating slaughter plants.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... upland game bird, commercial waterfowl, meat-type chicken, and meat-type turkey slaughter plants that are... participate in the Plan. (b) To participate in the Plan, meat-type chicken, meat-type turkey, and...

  7. 9 CFR 146.6 - Specific provisions for participating slaughter plants.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... upland game bird, commercial waterfowl, meat-type chicken, and meat-type turkey slaughter plants that are... participate in the Plan. (b) To participate in the Plan, meat-type chicken, meat-type turkey, and...

  8. 9 CFR 146.6 - Specific provisions for participating slaughter plants.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... upland game bird, commercial waterfowl, meat-type chicken, and meat-type turkey slaughter plants that are... participate in the Plan. (b) To participate in the Plan, meat-type chicken, meat-type turkey, and...

  9. 76 FR 48796 - Plan for Estimating Daily Livestock Slaughter Under Federal Inspection; Request for Extension of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-09

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF AGRICULTURE Agricultural Marketing Service Plan for Estimating Daily Livestock Slaughter Under Federal Inspection; Request for Extension of a Currently Approved Information Collection AGENCY: Agricultural Marketing...

  10. Effects of particle size and hydro-thermal treatment of feed on performance and stomach health in fattening pigs.

    PubMed

    Liermann, Wendy; Berk, Andreas; Böschen, Verena; Dänicke, Sven

    2015-01-01

    Effects of grinding and hydro-thermal treatment of feed on growth performance, slaughter traits, nutrient digestibility, stomach content and stomach health were examined by using 96 crossbred fattening pigs. Pigs were fed a grain-soybean meal-based diet processed by various technical treatments. Feeding groups differed in particle size after grinding (finely vs. coarsely ground feed) and hydro-thermal treatment (without hydro-thermal treatment, pelleting, expanding, expanding and pelleting). Fine grinding and hydro-thermal treatment showed significant improvements on the digestibility of crude nutrients and content of metabolisable energy. Hydro-thermal treatment influenced average daily gain (ADG) and average daily feed intake (DFI) significantly. Finely ground pelleted feed without expanding enhanced performances by increasing ADG and decreasing feed-to-gain ratio (FGR) of fattening pigs. Coarsely ground feed without hydro-thermal treatment resulted in the highest ADG and DFI, however also in the highest FGR. Expanded feed decreased DFI and ADG. Slaughter traits were not affected by treatments. Coarsely ground feed without hydro-thermal treatment had protective effects on the health of gastric pars nonglandularis, however, pelleting increased gastric lesions. Hydro-thermal treatment, especially expanding, resulted in clumping of stomach content which possibly induced satiety by slower ingesta passage rate and thus decreased feed intake. Pigs fed pelleted feed showed less pronounced development of clumps in stomach content compared with expanded feed.

  11. Physical, chemical and sensorial parameters for lambs of different groups, slaughtered at different weights.

    PubMed

    Landim, Aline Vieira; Castanheira, Marlos; Fioravanti, Maria Clorinda Soares; Pacheco, Aline; Cardoso, Maximiliano Tadeu Memória; Louvandini, Helder; McManus, Concepta

    2011-08-01

    The object of this experiment was to study physicochemical and sensorial traits in the 11th and 13th ribs of 24 Santa Ines (SI), 24 1/2 Ile de France × 1/2 Santa Inês (ILE × SI) and 8 1/2 Texel × 1/2 Santa Inês (TE × SI), slaughtered at different weights (30, 35, 40 and 45 kg, respectively). Subjective measurements (marbling, colour and texture) were carried out on the Longissimus dorsi, as well as initial pH (0 h) and final pH (24 h) after slaughter. The experiment was in a 3 × 4 factorial design and analysed using general linear model and correlation procedures in SAS®. Breed group did not influence colour (3.32), shear force (3.57 kg-force (kgf)) or loss in cooking (24.05%) of the L. dorsi, but slaughter weight affected these, with stronger colour, tougher meat and greater loss in cooking as slaughter weight increased. For sensorial analysis, the L. dorsi were cut, identified and evaluated by 30 untrained judges using a linear scale. Significant differences were detected in preference (6.61 points), tenderness (6.32 points), succulence (6.33 points) and flavour (7.08 points) of the meat from different breed groups and slaughter weights. For preference, all meats were well accepted although the crossbred animals slaughtered at 45 kg were less acceptable.

  12. Dietary tryptophan effects on plasma and salivary cortisol and meat quality in pigs.

    PubMed

    Guzik, A C; Matthews, J O; Kerr, B J; Bidner, T D; Southern, L L

    2006-08-01

    Four experiments were conducted to determine the effects of supplemental Trp on meat quality, plasma and salivary cortisol, and plasma lactate. Experiment 1 was a preliminary study to measure plasma cortisol concentrations in 4 barrows (50 kg of BW) that were snared for 30 s at time 0 min. Pigs were bled at -60, -30, -15, 2, 4, 6, 8, 10, 15, 20, 25, 30, 45, 60, 90, and 120 min. Plasma cortisol was near maximum 10 min after the pigs were snared. In Exp. 2, 20 barrows (50 kg of BW) were allotted to a basal corn-soybean meal diet or the basal diet with 0.5% supplemental l-Trp for 5 d. After the 5-d feeding period, pigs were snared for 30 s and bled at -10, 0, 2, 4, 6, 8, 10, 15, 20, 25, 30, 45, 60, 90, and 120 min after snaring. Pigs fed the diet with supplemental Trp had a lower (P < 0.01) mean plasma cortisol than pigs fed the basal diet. Plasma lactate also was decreased (P < 0.07) by supplemental Trp. In Exp. 3, the same pigs and treatments were used as in Exp. 2, but 5 pigs were snared and 15 pigs adjacent to those being snared were bled to determine if pigs are stressed when they are adjacent to pigs being snared. For pigs adjacent to snared pigs, the area under the curve (P < 0.06) and mean for plasma cortisol was lower (P < 0.01) in pigs fed Trp relative to those fed the basal diet. In Exp. 4, 90 barrows (initial BW of 106 kg) were allotted to 6 treatments in a 3 x 2 factorial arrangement. Three diets with Trp (basal diet, basal supplemented with 0.5% Trp for 5 d, or pigs fed the basal diet with a 0.1 g/kg of BW Trp bolus given 2 h before slaughter) were combined with 2 handling methods (minimal and normal handling). Dressing percent, 24-h pH, and 24-h temperature were reduced in the minimally handled pigs (P < 0.10) compared with the normally handled pigs. Pigs fed Trp in the diet relative to those fed the basal diet had increased 45-min temperature, Commission Internationale de l'Eclairage (CIE) redness (a*) and yellowness (b*) values, and drip and total

  13. Performance of growing pigs fed diets based on by-products of maize and wheat processing.

    PubMed

    Mwesigwa, Robert; Mutetikka, David; Kugonza, Donald Rugira

    2013-02-01

    Forty-eight crossbred Landrace × Large white pigs with an average body weight of 8.5 ± 0.4 kg and aged 2 months were used to evaluate performance and carcass characteristics of pigs fed two diets in which bran from maize or wheat was used as the energy source. A third diet based on whole maize grain was used as a control. Animals in groups of four, balanced for litter, sex and weight were allotted to dietary treatments in a completely randomised design with four replications. Data were collected on feed intake and weight gain for a period of 4 months. A digestibility trial was carried at the end of the feeding trial using two male pigs per treatment while six pigs per treatment were randomly selected for slaughter to determine carcass characteristics. Daily gain averaged 0.23, 0.31 and 0.13 kg/day, for pigs fed maize bran, wheat bran and whole maize diets, respectively. Average feed intake and final body weight were significantly (P < 0.05) affected by diets. Digestibility of dry matter, crude fibre and calcium were higher (P < 0.05) for pigs fed maize bran while crude protein digestibility was highest (P < 0.05) for the pigs fed wheat bran. There were significant differences (P < 0.05) between the diets in carcass yield, ham, head, trotters and heart weights and also for rib weight, rib eye muscle and other tissues. This study indicates that comparable performance of growing pigs fed cereal bran and full-grain diets are real.

  14. Studies of the field efficacy and safety of a single-dose Mycoplasma hyopneumoniae vaccine for pigs.

    PubMed

    Dawson, A; Harvey, R E; Thevasagayam, S J; Sherington, J; Peters, A R

    2002-11-02

    The field efficacy and safety of a single-dose Mycoplasma hyopneumoniae vaccine were evaluated in three-to five-week-old pigs. Two field efficacy studies were conducted, one in England with 673 pigs, and one in Germany with 719 pigs. The pigs were injected intramuscularly with either the vaccine or saline (control) at a ratio of 2:1 and reared under commercial conditions to slaughter weight. The efficacy of the vaccine was evaluated by comparing the lung lesions associated with infection with M. hyopneumoniae in the control and vaccinated animals postmortem. In both countries the vaccinated pigs had a significantly lower percentage of lung lesion scores, in England 5.7 v 10.2 per cent (P = 0.0022) and in Germany 3.9 v 7.7 per cent (P = 0.0056). In Germany the average daily weight gain (ADG) of the vaccinated pigs was significantly higher (639 g v 616 g) (P = 0.0205). In both countries and in both the treated and control animals there was a significant negative correlation between the ADG and the lung lesion score (P = 0.0001). Two safety trials were conducted, one in England and one in Germany, each with 75 pigs, and in each case 50 pigs were given the maximum batch release antigen titre of the vaccine and 25 were given saline. The safety of the vaccine was evaluated by observation for local and systemic reactions and any increases in rectal temperature. No abnormal reactions were observed in the vaccinated pigs and there was no significant difference between the mean peak rectal temperatures of the vaccinated and control pigs in either trial.

  15. Unveiling contamination sources and dissemination routes of Salmonella sp. in pigs at a Portuguese slaughterhouse through macrorestriction profiling by pulsed-field gel electrophoresis.

    PubMed

    Vieira-Pinto, Madalena; Tenreiro, Rogério; Martins, Conceição

    2006-07-01

    Sixty-nine isolates of Salmonella sp. isolated from the ileum, tonsils, carcass and mandibular and ileocolic lymph nodes of individual pigs slaughtered for consumption in one abattoir were analyzed using serotyping and macrorestriction profiling by Pulsed-Field Gel Electrophoresis (RFLP-PFGE), in order to identify clonal relationships. XbaI macrorestriction was able to distinguish 18 genotypes among the eight identified serotypes: Salmonella Typhimurium (4 genotypes), Salmonella Rissen (3), Salmonella Tennessee (2), Salmonella Enteritidis (2), Salmonella 4,[5],12:i:- (4), Salmonella Give (1), Salmonella Anatum (1), and Salmonella Derby (1). Except for one sample, the serotype and the genotype identified in the samples from the same pork were always the same, allowing to unravel possible dissemination routes of Salmonella sp. through these pork tissues and equate presumptive sources of contamination or infection. Highly significant associations (p < 0.001) were observed for the presence of Salmonella sp. in the ileum and in the ileocolic lymph nodes, as well as between the carcass contamination and the presence of Salmonella sp. in others samples of the correspondent slaughtered pig, such as the ileum, the ileocolic and mandibular lymph nodes and the tonsils. Moreover, 80% of the pigs with ileum and ileocolic lymph nodes positive samples also presented the same salmonella genotype in the correspondent tonsils and, among pigs with positive tonsils, 70% also carried the same genotype in the corresponding mandibular lymph nodes. The occurrence of cross-contamination was also detected, since a genotype identified in other pigs slaughtered in the same day was found in 31% of positive carcasses. The global analysis of the genotypes suggested three different sources of pig infection: the farm of origin, the transportation and the lairage. A particular attention should be paid to the last one, since the majority of the isolates from pig samples were related to infection in

  16. Pig production in the Solomon Islands. I. Village pig production.

    PubMed

    de Fredrick, D F

    1977-05-01

    In 181 villages in the Solomon Islands the pig: human ratio was 1:5-8 and the annual per capita pork consumption was 4-2 kg. Some communities did not keep pigs or eat pig meat. Sows weaned an average of 5-5 piglets per year and mean liveweight at 12 months of age was 28-4 kg. Most pigs were kept on the ground but some were housed in pens over the sea and very few lived in their owner's houses. Pigs were important in the social life of the people but proportionally fewer pigs were raised than in neighbouring Pacific countries.

  17. Echinococcosis in pigs and intestinal infection with Echinococcus spp. in dogs in southwestern Lithuania.

    PubMed

    Bruzinskaite, R; Sarkūnas, M; Torgerson, P R; Mathis, A; Deplazes, P

    2009-03-23

    Cystic echinococcosis is a major emerging zoonosis in many Eastern European and Asian countries. Post slaughter examinations of 684 pig livers in Lithuania revealed significantly higher numbers of Echinococcus granulosus infections in animals from family farms (13.2%; 95% CI 10.7-16.2) as compared with those from industrial farms (4.1%; 95% CI 0.8-11.5). The prevalence was also significantly higher in pigs older than 1 year than in younger ones. In addition, in 0.5% of the pigs from the family farms, infertile and calcified E. multilocularis lesions were identified by PCR. Faecal samples from rural dogs (n=240) originating from 177 family farms in 12 villages were investigated for taeniid eggs with two methods. Significantly more dogs excreting taeniid eggs were diagnosed with the flotation/sieving method (n=34) as compared to the modified McMaster method (n=12). Multiplex PCR performed with DNA from taeniid eggs isolated from faeces of 34 dogs revealed 26 infections with Taenia spp., 9 with E. granulosus and 2 with E. multilocularis (4 cases with concurrent Taenia spp. and E. granulosus or E. multilocularis infections). Genotyping of E. granulosus cyst tissues from 7 pigs, 1 head of cattle and from E. granulosus eggs from 8 dog faeces revealed the genotype G6/7 ('pig/camel strain') in all cases. The high infection pressure with Echinococcus spp. in family farms necessitates initiating control programs.

  18. Induction of seroconversion and persistence of Salmonella Typhimurium in pigs are strain dependent.

    PubMed

    Van Parys, Alexander; Boyen, Filip; Leyman, Bregje; Verbrugghe, Elin; Maes, Dominiek; Haesebrouck, Freddy; Pasmans, Frank

    2013-09-01

    Foodborne salmonellosis is one of the most important bacterial zoonotic diseases worldwide. Salmonella Typhimurium is the serovar most frequently isolated from persistently infected slaughter pigs in Europe. Salmonella Typhimurium pathogenesis is host species specific. In addition, differences in in vitro behaviour of Salmonella Typhimurium strains have also been described, which may be reflected by a different course of infection within a host species. We compared the course of a Salmonella Typhimurium infection in pigs, using two Salmonella Typhimurium strains that were able to interfere with MHC II expression on porcine macrophages to a different extent in vitro. After experimental inoculation, blood and faecal samples from all pigs were collected at regular time points. At 40 days post inoculation (pi), animals were euthanized and tissue samples were bacteriologically analysed. The proportion of serologically positive piglets at 33 days pi was significantly higher in pigs that were inoculated with the strain that did not downregulate MHC II expression in vitro. Furthermore, this strain was less frequently shed and isolated in lower numbers from tonsils and ileocaecal lymph nodes than the strain that was able to markedly downregulate MHC II expression in vitro. We thus found that the delayed onset of seroconversion after oral inoculation of piglets with a particular Salmonella Typhimurium strain coincided with higher faecal shedding and increased persistence. Strain specific differences in Salmonella pathogenesis might thus have repercussions on the serological detection of Salmonella Typhimurium infections in pigs.

  19. The perception and acceptability of pre-slaughter and post-slaughter stunning for Halal production: The views of UK Islamic scholars and Halal consumers.

    PubMed

    Fuseini, Awal; Wotton, Steve B; Hadley, Phil J; Knowles, Toby G

    2017-01-01

    The importance of religious slaughter from economic, emotive and ethical viewpoints is significant. There are apparent economic benefits associated with trading in meats slaughtered according to religious traditions. Some religious authorities insist on the slaughter of animals without stunning, but this, according to many researchers, compromises animal welfare. We conducted a survey of Islamic scholars and Halal consumers, 66 scholars from 55 organisations and 314 consumers from 54 UK cities/towns were surveyed. Forty-nine scholars were interviewed through pre-arranged meetings, 17 surveyed online whilst all 314 consumers were either surveyed online or through the remote completion of copies of the questionnaire. Most of the scholars (>95%) (CI 86.9 to 98.4%) agreed that if an animal is stunned and then slaughtered by a Muslim and the method of stunning does not result in death, cause physical injury or obstruct bleed-out, the meat would be Halal and 53% (CI 47 to 58%) consumers also thought such meat would be Halal.

  20. Assessment of food safety risks associated with preslaughter activities during the traditional slaughter of goats in Gauteng, South Africa.

    PubMed

    Qekwana, Nenene Daniel; Oguttu, James Wabwire

    2014-06-01

    The South African Department of Agriculture, Forestry and Fisheries estimated in 2012 that there were 2.033 million goats in the country. Of these animals, less than 0.5% are slaughtered at registered abattoirs. Although informal and traditional slaughter of goats for home consumption is permitted under the South African Meat Safety Act 40 of 2000, the responsibility for ensuring that products are safe is left to the traditional or ritual slaughter practitioners. The objective of the present study was to assess whether preslaughter activities associated with traditional or ritual slaughter promote or reduce food-associated risks and to recommend mitigation strategies for potential food safety hazards. Structured interviews were conducted with 105 selected respondents (in and around Tshwane, South Africa) who had been involved in traditional goat slaughter. Approximately 70% of goats slaughtered were obtained from sources that could be traced to ascertain the origin of the goats. None of the respondents were aware of the need for a health declaration for slaughter stock. Some slaughter practitioners (21%) perform prepurchase inspection of stock to ascertain their health status. However, this percentage is very small, and the approach is based on indigenous knowledge systems. The majority of respondents (67.6%) travelled 1 to 11 km to obtain a goat for traditional slaughter. Although approximately 70% of slaughter goats were transported by vehicles, the vehicles used did not meet the legal standard. More than two-thirds of goats were tied to a tree while waiting to be slaughtered, and the rest were held in a kraal. The holding period ranged from 1 to 72 h, but more than 70% of the animals were slaughtered within 36 h. This study revealed that traditional and ritual slaughter involves some preslaughter activities with potential to mitigate the risk of slaughtering animals that are not fit for human consumption. Such activities include prepurchase inspection, obtaining

  1. Growth performance and whole-body composition of pigs experimentally infected with Mycoplasma hyopneumoniae.

    PubMed

    Escobar, J; Van Alstine, W G; Baker, D H; Johnson, R W

    2002-02-01

    Mycoplasma hyopneumoniae (Mh) is the primary infectious pathogen responsible for enzootic pneumonia in pigs. Although Mh is thought to impair growth performance, whole-body composition, and fat and protein accretion in pigs with pneumonia have not been reported and the mechanism through which Mh reduces growth is unknown. The objectives of this study were to evaluate the effects of Mh on growth performance, whole-body composition, and protein and fat accretion in nursery pigs and to determine whether Mh infection increases the expression of interleukin (IL)-1beta, IL-6, and tumor necrosis factor-alpha (TNF-alpha). Sixty-four 2-wk-old Mh-free pigs were used (two trials) in a randomized complete block design. In each trial, two pigs were housed in each of 16 disease-containment chambers. At 4 wk of age, pigs were inoculated intratracheally with 3 mL of Mh broth (P5722-3, 10(7) cfu/mL) or sterile Friis culture medium. Clinical signs of disease and feed intake were monitored daily and body weight was determined weekly for 4 wk. Whole-body composition was determined from pigs killed 0, 14, and 28 d after inoculation, and the comparative slaughter technique was used to estimate protein and fat accretion. At death, gross lung lesions were quantified, and lung tissue was collected to verify the presence or absence of Mh, and to determine cytokine mRNA levels. Control pigs displayed no overt signs of infection and were Mh-negative and free of pulmonary lesions. Pigs inoculated with Mh showed pneumonic coughing (P < 0.005), were Mh-positive, and had pulmonary lesions that affected 4.5% (P < 0.01) and 14.1% (P < 0.001) of total lung surface area at 14 and 28 d, respectively, after inoculation. Ribonuclease protection assays revealed increased IL-1beta (P < 0.04) and TNF-alpha (P < 0.06) mRNA in lung tissue collected from a lesion site compared with tissue collected 10 cm from a lesion site or from control pigs. Interestingly, Mh did not depress weight gain or feed efficiency

  2. Taenia hydatigena in pigs in Burkina Faso: A cross-sectional abattoir study.

    PubMed

    Dermauw, Veronique; Ganaba, Rasmané; Cissé, Assana; Ouedraogo, Boubacar; Millogo, Athanase; Tarnagda, Zékiba; Hul, Anke Van; Gabriël, Sarah; Carabin, Hélène; Dorny, Pierre

    2016-10-30

    Taenia hydatigena is a non-zoonotic cestode that has canines as definitive hosts and ruminants and pigs as intermediate hosts. In pigs, its presence causes cross-reactivity in serological testing for Taenia solium cysticercosis. Therefore, knowledge on the occurrence of T. hydatigena is paramount for validly estimating the seroprevalence of T. solium cysticercosis in pigs. In a cross-sectional abattoir study, we estimated the prevalence of T. hydatigena in pigs slaughtered in Koudougou, Burkina Faso. Carcasses of 452 pigs were examined by investigators for perceived and suspected T. hydatigena cysticercus lesions in the abdominal cavity or on the surface of abdominal organs. Routine meat inspection was performed by local inspectors to identify T. solium cysticerci. All lesions were subjected to PCR-RFLP analysis in order to differentiate Taenia spp. Additionally, individual blood samples were examined for the presence of circulating cysticercus antigens using the B158/B60 Ag-ELISA. Perceived T. hydatigena cysticerci were found in 13 pigs, whereas meat inspectors found seven carcasses infected with T. solium cysticerci. All were confirmed by molecular analysis. Of pigs with other suspected lesions, mostly located in the liver, 27 and six were found to harbour T. hydatigena and T. solium cysticerci, respectively. Overall, 8.8% of pigs (40/452) were found infected with T. hydatigena and 2.9% (13/452) with T. solium. Of these positive pigs, one was found infected with both Taenia spp. (0.2%, 1/452). Blood samples of 48.5% of pigs (219/452) were positive in the Ag-ELISA. Pigs with confirmed cysts of T. hydatigena and T. solium had a positive Ag-ELISA result in 57.5% (23/40) and 61.5% (8/13) of cases, respectively. The observed T. hydatigena prevalence in this study is relatively high in comparison to other studies in Africa. Estimates of the occurrence of active porcine T. solium infection using the B158/B60 Ag-ELISA should therefore be adjusted for the presence of T

  3. Effect of age at the beginning of the free-range fattening period on growth and carcass and fat quality in Iberian pigs.

    PubMed

    Daza, Argimiro; López-Bote, Clemente; Rey, Ana; Olivares, Alvaro

    2006-08-01

    This experiment was carried out to study the influence of age at the beginning of the free-range fattening period (traditional pigs, TP, age 12 months vs. young pigs, YP, age 8 months) on the performance of Iberian pigs. During 152 days prior to the fattening period, TP and YP pigs received 1.7 and 2.6 kg feed per day, respectively. During fattening, TP pigs had a higher average daily gain (p < 0.05) than YP pigs. The proportions of PUFA and n-3 fatty acids of the outer and inner layers of subcutaneous backfat were higher in TP than in YP pigs (p < 0.05), while the proportions of C16:0 and SFA in the inner layer of subcutaneous backfat were greater in YP than in TP pigs (p < 0.05). The ratio of n-6/n-3 in subcutaneous backfat was lower in TP than in YP pigs (p < 0.05). The percentage of intramuscular fat in longissimus dorsi muscle was higher in TP than in YP pigs (p < 0.05). The relationship between the percentage of intramuscular fat in longissimus dorsi muscle and average daily gain during the free-range fattening period adjusted to a quadratic function (p < 0.05). The concentration of alpha- and gamma-tocopherol in subcutaneous backfat at slaughter was significantly higher in TP than in YP pigs (p < 0.05). It is concluded that Iberian pigs that have 8 months of age at the beginning of free-range feeding have adequate commercial quality.

  4. Danish Colorectal Cancer Group Database

    PubMed Central

    Ingeholm, Peter; Gögenur, Ismail; Iversen, Lene H

    2016-01-01

    Aim of database The aim of the database, which has existed for registration of all patients with colorectal cancer in Denmark since 2001, is to improve the prognosis for this patient group. Study population All Danish patients with newly diagnosed colorectal cancer who are either diagnosed or treated in a surgical department of a public Danish hospital. Main variables The database comprises an array of surgical, radiological, oncological, and pathological variables. The surgeons record data such as diagnostics performed, including type and results of radiological examinations, lifestyle factors, comorbidity and performance, treatment including the surgical procedure, urgency of surgery, and intra- and postoperative complications within 30 days after surgery. The pathologists record data such as tumor type, number of lymph nodes and metastatic lymph nodes, surgical margin status, and other pathological risk factors. Descriptive data The database has had >95% completeness in including patients with colorectal adenocarcinoma with >54,000 patients registered so far with approximately one-third rectal cancers and two-third colon cancers and an overrepresentation of men among rectal cancer patients. The stage distribution has been more or less constant until 2014 with a tendency toward a lower rate of stage IV and higher rate of stage I after introduction of the national screening program in 2014. The 30-day mortality rate after elective surgery has been reduced from >7% in 2001–2003 to <2% since 2013. Conclusion The database is a national population-based clinical database with high patient and data completeness for the perioperative period. The resolution of data is high for description of the patient at the time of diagnosis, including comorbidities, and for characterizing diagnosis, surgical interventions, and short-term outcomes. The database does not have high-resolution oncological data and does not register recurrences after primary surgery. The Danish

  5. Cross-Sectional Study of Toxoplasma gondii Infection in Pig Farms in England.

    PubMed

    Limon, Georgina; Beauvais, Wendy; Dadios, Nikolaos; Villena, Isabelle; Cockle, Charlotte; Blaga, Radu; Guitian, Javier

    2017-02-10

    Ingestion of undercooked meat has been proposed as an important source of human Toxoplasma gondii infection. To ascertain the contribution of meat consumption to the risk of human infection, estimates of the prevalence of infection in meat-producing animals are required. A cross-sectional study was conducted to assess T. gondii infection in pigs raised in England, to identify risk factors for infection, and to compare performance of two serological tests: modified agglutination test (MAT) and enzyme-linked immunosorbent assay (ELISA). Blood samples from 2071 slaughter pigs originating from 131 farms were collected and 75 (3.6%) were found to be positive by MAT. Positive pigs originated from 24 farms. A subset of samples (n = 492) were tested using ELISA, and a significant disagreement (p < 0.001) was found between the two tests. An empirical Bayes approach was used to estimate the farm-level prevalence and the probability of each individual farm having at least one positive animal, considering the uncertainty arising from the sampling strategy and the imperfect test performance. The adjusted farm-level prevalence was 11.5% (95% credible interval of positive farms 8.4-16.0%). Two different criteria were used for classifying farms as infected: (1) ≥50% probability of having at least one infected pig (n = 5, 6.8%) and (2) ≥10% probability (n = 15, 20.5%). Data on putative risk factors were obtained for 73 farms. Using a 10% cutoff, the relative risk (RR) of infection was higher in farms where cats have direct access to pigs' food (RR = 2.6; p = 0.04), pigs have outdoor access (RR = 3.0; p = 0.04), and farms keeping ≤200 pigs (RR = 3.9; p = 0.02), with strong collinearity between the three variables. The findings suggest a low level of T. gondii infection in the farms studied, most of which are likely to send to slaughter batches comprising 100% uninfected pigs. These results provide key inputs to quantitatively assess the

  6. Mycotoxic nephropathy in pigs*

    PubMed Central

    Elling, F.; Møller, T.

    1973-01-01

    In Denmark a nephropathy in pigs characterized by tubular atrophy and interstitial fibrosis has been identified frequently during the last 5 decades in the course of meat inspection in slaughterhouses. The disease was first described by Larsen, who recognized the connexion between feeding mouldy rye to pigs and the development of the nephropathy. In this study kidneys were examined from 19 pigs coming from a farm with an outbreak of nephropathy. The barley fed to the pigs was contaminated with the mycotoxin ochratoxin A. Histological examination revealed different degrees of change ranging from slight regressive changes in the tubular epithelium and periglomerular and interstitial fibrosis to tubular atrophy, thickened basement membranes, glomerular sclerosis, and marked fibrosis. These differences were considered to be due to differences in the length of time of exposure to the mouldy barley and differences in the amount of mycotoxin consumed by the individual pig. However, it will be necessary to carry out experiments using crystalline ochratoxin A in order to prove such a relationship. Mycotoxins have also been suggested as etiological factors in Balkan nephropathy in man, which in the initial stages is characterized by tubular lesions similar to those seen in mycotoxic nephropathy in pigs. ImagesFig. 1Fig. 2Fig. 7Fig. 8Fig. 9Fig. 3Fig. 4Fig. 5Fig. 6Fig. 10Fig. 11 PMID:4546872

  7. Cattle cruelty and risks of meat contamination at Akinyele cattle market and slaughter slab in Oyo State, Nigeria.

    PubMed

    Adeyemo, Olanike K; Adeyemi, Isaac G; Awosanya, Emmanuel J

    2009-12-01

    Cattle transported to the government-run cattle market and slaughter slab in Akinyele, Oyo State, Nigeria on their final voyage are facing a disturbingly cruel, filthy and unsafe environment that is also raising the risk of contamination of meat sold for human consumption. This report gives a picture of what the cattle have to go through before they are slaughtered. This study also reveals cattle awaiting slaughter in abysmal health conditions, cows pulled with extreme force towards lairage and slaughter slab. Equally disturbing is the filthy situation inside the abattoir where the risk of contamination of meat is significant. Also, poor meat handling, transportation and sales practices subject meat to contamination leading to poor quality and exposure of human consumers to health risk. Development of hygienic slaughter slab operations, improved transportation system for both livestock and meat is therefore recommended; not only for Akinyele, but all abattoirs and slaughter slabs in Nigeria.

  8. Composition and quality characteristics of carcasses from pigs divergently selected for residual feed intake on high- or low-energy diets.

    PubMed

    Arkfeld, E K; Young, J M; Johnson, R C; Fedler, C A; Prusa, K; Patience, J F; Dekkers, J C M; Gabler, N K; Lonergan, S M; Huff-Lonergan, E

    2015-05-01

    The objective was to determine the extent to which feeding low-energy, high-fiber (LEHF) and high-energy, low-fiber (HELF) diets impacts meat quality and carcass composition of pigs divergently selected for residual feed intake (RFI). Two experiments were conducted in the divergently selected Iowa State University RFI lines: Exp. 1 evaluated carcasses of generation (G) 8 pigs fed on commercial feeders; Exp. 2 evaluated composition, pork quality, sensory, and postmortem proteolysis of pigs fed on electronic single-space feeders in G 8 and 9. Pigs (N = 177) in Exp. 1 were randomly assigned a pen (mixed sex and line; N = 8). Groups (n = 3) of pigs were slaughtered at a mean BW of 121.5 kg. Pigs in Exp. 2 (G8: n = 158; G9: n = 157) were randomly assigned to 1 of 6 pens of each diet per G. Pigs from G8 were slaughtered at a mean BW of 122.5 kg and G9 at a mean of 128.4 kg. Data were analyzed using the mixed procedure of SAS. Fixed effects were line, diet, sex, and all appropriate interactions. Random effects were group, pen, litter, and sire and covariate of off-test BW. For Exp. 2, G was added as a fixed effect and sensory day was added as a random effect when applicable. In Exp. 1, carcasses from low RFI (LRFI) pigs were leaner and had less fat depth (P < 0.01). Carcasses from pigs fed the LEHF diet had a lighter HCW and greater estimated percent lean than pigs fed HELF diet (P < 0.01). In Exp. 2, LRFI pigs on the HELF diet had the greatest loin depth (P < 0.01). Chops from HRFI pigs had greater drip loss, color scores, lean tissue a*, and percent lipid and lesser percent moisture than LRFI ( P< 0.05). Chops from pigs on the LEHF diet had lesser muscle L* values and greater percent moisture than chops from pigs fed the HELF diet (P < 0.05). Chops from LRFI pigs were juicer than those from HRFI pigs (P < 0.05). Protein extracted at d 2 postmortem from LRFI pigs on the LEHF diet had a greater 38 kDa desmin degradation product than protein from LRFI pigs fed the HELF

  9. The Danish Bladder Cancer Database

    PubMed Central

    Hansen, Erik; Larsson, Heidi; Nørgaard, Mette; Thind, Peter; Jensen, Jørgen Bjerggaard

    2016-01-01

    Aim of database The aim of the Danish Bladder Cancer Database (DaBlaCa-data) is to monitor the treatment of all patients diagnosed with invasive bladder cancer (BC) in Denmark. Study population All patients diagnosed with BC in Denmark from 2012 onward were included in the study. Results presented in this paper are predominantly from the 2013 population. Main variables In 2013, 970 patients were diagnosed with BC in Denmark and were included in a preliminary report from the database. A total of 458 (47%) patients were diagnosed with non-muscle-invasive BC (non-MIBC) and 512 (53%) were diagnosed with muscle-invasive BC (MIBC). A total of 300 (31%) patients underwent cystectomy. Among the 135 patients diagnosed with MIBC, who were 75 years of age or younger, 67 (50%) received neoadjuvent chemotherapy prior to cystectomy. In 2013, a total of 147 patients were treated with curative-intended radiation therapy. Descriptive data One-year mortality was 28% (95% confidence interval [CI]: 15–21). One-year cancer-specific mortality was 25% (95% CI: 22–27%). One-year mortality after cystectomy was 14% (95% CI: 10–18). Ninety-day mortality after cystectomy was 3% (95% CI: 1–5) in 2013. One-year mortality following curative-intended radiation therapy was 32% (95% CI: 24–39) and 1-year cancer-specific mortality was 23% (95% CI: 16–31) in 2013. Conclusion This preliminary DaBlaCa-data report showed that the treatment of MIBC in Denmark overall meet high international academic standards. The database is able to identify Danish BC patients and monitor treatment and mortality. In the future, DaBlaCa-data will be a valuable data source and expansive observational studies on BC will be available. PMID:27822081

  10. The Danish Prostate Cancer Database

    PubMed Central

    Nguyen-Nielsen, Mary; Høyer, Søren; Friis, Søren; Hansen, Steinbjørn; Brasso, Klaus; Jakobsen, Erik Breth; Moe, Mette; Larsson, Heidi; Søgaard, Mette; Nakano, Anne; Borre, Michael

    2016-01-01

    Aim of database The Danish Prostate Cancer Database (DAPROCAdata) is a nationwide clinical cancer database that has prospectively collected data on patients with incident prostate cancer in Denmark since February 2010. The overall aim of the DAPROCAdata is to improve the quality of prostate cancer care in Denmark by systematically collecting key clinical variables for the purposes of health care monitoring, quality improvement, and research. Study population All Danish patients with histologically verified prostate cancer are included in the DAPROCAdata. Main variables The DAPROCAdata registers clinical data and selected characteristics for patients with prostate cancer at diagnosis. Data are collected from the linkage of nationwide health registries and supplemented with online registration of key clinical variables by treating physicians at urological and oncological departments. Main variables include Gleason scores, cancer staging, prostate-specific antigen values, and therapeutic measures (active surveillance, surgery, radiotherapy, endocrine therapy, and chemotherapy). Descriptive data In total, 22,332 patients with prostate cancer were registered in DAPROCAdata as of April 2015. A key feature of DAPROCAdata is the routine collection of patient-reported outcome measures (PROM), including data on quality-of-life (pain levels, physical activity, sexual function, depression, urine and fecal incontinence) and lifestyle factors (smoking, alcohol consumption, and body mass index). PROM data are derived from questionnaires distributed at diagnosis and at 1-year and 3-year follow-up. Hitherto, the PROM data have been limited by low completeness (26% among newly diagnosed patients in 2014). Conclusion DAPROCAdata is a comprehensive, yet still young clinical database. Efforts to improve data collection, data validity, and completeness are ongoing and of high priority. PMID:27843346

  11. Pigs that are divergent in feed efficiency, differ in intestinal enzyme and nutrient transporter gene expression, nutrient digestibility and microbial activity.

    PubMed

    Vigors, S; Sweeney, T; O'Shea, C J; Kelly, A K; O'Doherty, J V

    2016-11-01

    Feed efficiency is an important trait in the future sustainability of pig production, however, the mechanisms involved are not fully elucidated. The objective of this study was to examine nutrient digestibility, organ weights, select bacterial populations, volatile fatty acids (VFA's), enzyme and intestinal nutrient transporter gene expression in a pig population divergent in feed efficiency. Male pigs (n=75; initial BW 22.4 kg SEM 2.03 kg) were fed a standard finishing diet for 43 days before slaughter to evaluate feed intake and growth for the purpose of calculating residual feed intake (RFI). Phenotypic RFI was calculated as the residuals from a regression model regressing average daily feed intake (ADFI) on average daily gain (ADG) and midtest BW0.60 (MBW). On day 115, 16 pigs (85 kg SEM 2.8 kg), designated as high RFI (HRFI) and low RFI (LRFI) were slaughtered and digesta was collected to calculate the coefficient of apparent ileal digestibility (CAID), total tract nutrient digestibility (CATTD), microbial populations and VFA's. Intestinal tissue was collected to examine intestinal nutrient transporter and enzyme gene expression. The LRFI pigs had lower ADFI (P<0.001), improved feed conversion ratio (P<0.001) and an improved RFI value relative to HRFI pigs (0.19 v. -0.14 SEM 0.08; P<0.001). The LRFI pigs had an increased CAID of gross energy (GE), and an improved CATTD of GE, nitrogen and dry matter compared to HRFI pigs (P<0.05). The LRFI pigs had higher relative gene expression levels of fatty acid binding transporter 2 (FABP2) (P<0.01), the sodium/glucose co-transporter 1 (SGLT1) (P<0.05), the glucose transporter GLUT2 (P<0.10), and the enzyme sucrase-isomaltase (SI) (P<0.05) in the jejunum. The LRFI pigs had increased populations of lactobacillus spp. in the caecum compared with HRFI pigs. In colonic digesta HRFI pigs had increased acetic acid concentrations (P<0.05). Differences in nutrient digestibility, intestinal microbial populations and gene

  12. Factors associated with age at slaughter and carcass weight, price, and value of dairy cull cows.

    PubMed

    Bazzoli, I; De Marchi, M; Cecchinato, A; Berry, D P; Bittante, G

    2014-02-01

    The sale of cull cows contributes to the overall profit of dairy herds. The objective of this study was to quantify the factors associated with slaughter age (mo), cow carcass weight (kg), price (€/kg of carcass weight), and value (€/head) of dairy cull cows. Data included 20,995 slaughter records in the period from 2003 to 2011 of 5 different breeds: 2 dairy [Holstein Friesian (HF) and Brown Swiss (BS)] and 3 dual-purpose [Simmental (Si), Alpine Grey (AG), and Rendena (Re)]. Associations of breed, age of cow (except when the dependent variable was slaughter age), and year and month of slaughter with slaughter age, carcass weight, price, and value were quantified using a mixed linear model; herd was included as a random effect. The seasonal trends in cow price and value traits were inversely related to the number of cows slaughtered, whereas annual variation in external factors affected market conditions. Relative to BS cows, HF cows were younger at slaughter (73.1 vs. 80.7 mo), yielded slightly lighter carcasses (242 vs. 246 kg), and received a slightly lower price (1.69 vs. 1.73 €/kg) and total value (394 vs. 417 €/head). Dual-purpose breeds were older and heavier and received a much greater price and total value at slaughter (521, 516, and 549 €/head, respectively for Si, Re, and AG) than either dairy breed. Of the dual-purpose cows, Si carcasses were heavier (271 kg), whereas the carcasses of local breeds received a higher price (2.05 and 2.18 €/kg for Re and AG, respectively) and Alpine Grey cows were the oldest at slaughter (93.3 mo). The price per kilogram of cull cow carcasses was greatest for very young cows (i.e., <3 yr of age) and the differential in price and value between younger and older cows was greater in dual-purpose than in dairy breeds. Large differences in cull cow whole carcass value (carcass weight × unit price) among dairy breeds suggest that such a trait could be considered in the breeding objectives of the breeds.

  13. Validation of indicators used to assess unconsciousness in veal calves at slaughter.

    PubMed

    Verhoeven, M T W; Gerritzen, M A; Hellebrekers, L J; Kemp, B

    2016-09-01

    European legislation states that after stunning regular checks should be performed to guarantee animals are unconscious between the end of the stunning process and death. When animals are killed without prior stunning these checks should be performed before the animal is released from restraint. The validity of certain indicators used to assess unconsciousness under different stunning and slaughter conditions is under debate. The aim of this study was to validate the absence of threat-, withdrawal-, corneal- and eyelid reflex as indicators to assess unconsciousness in calves subjected to different stunning and slaughter methods. Calves (201±22 kg) were randomly assigned to one of the following four treatments: (1) Captive bolt stunning followed by neck cut in an inverted position (n=25); (2) Non-stunned slaughter in an upright position (n=7); (3) Non-stunned slaughter in an inverted position (180° rotation) (n=25); (4) Non-stunned slaughter in an upright position followed by captive bolt stunning 40 s after the neck cut (n=25). Each calf was equipped with non-invasive electroencephalogram (EEG) electrodes before the slaughter procedure. All reflexes were verified once before the slaughter procedure. At the beginning of the procedure (T=0 s) calves were stunned (treatment 1) or neck cut in an upright position (treatment 2, 4) or inverted position (treatment 3). Calves of treatment 4 were captive bolt stunned 34±8 s after the neck cut. Reflexes were assessed every 20 s from T=15 s for all treatments until all reflex tests resulted in a negative response three times in a row and a flat line EEG was observed. In addition, reflexes were assessed 5 s after captive bolt stunning in calves of treatments 1 and 4. Visual assessment of changes in the amplitude and frequency of EEG traces was used to determine loss of consciousness. Timing of loss of consciousness was related to timing of loss of reflexes. After captive bolt stunning, absence of threat-, withdrawal

  14. Prevalence of Taenia solium cysticercosis in pigs entering the food chain in western Kenya.

    PubMed

    Thomas, Lian Francesca; Harrison, Leslie Jayne Stevenson; Toye, Philip; de Glanville, William Anson; Cook, Elizabeth Anne Jesse; Wamae, Claire Njeri; Fèvre, Eric Maurice

    2016-01-01

    Three hundred forty-three pigs slaughtered and marketed in western Kenya were subjected to lingual examination and HP10 Ag-ELISA for the serological detection of Taenia solium antigen. When estimates were adjusted for the sensitivity and specificity of the diagnostic assays, prevalence of T. solium cysticercosis estimated by lingual exam and HP10 Ag-ELISA was between 34.4% (95% confidence interval (CI) 19.4-49.4%) and 37.6% (95% CI 29.3-45.9%), respectively. All pigs, however, were reported to have passed routine meat inspection. Since T. solium poses a serious threat to public health, these results, if confirmed, indicate that the introduction of control strategies may be appropriate to ensure the safety of pork production in this region.

  15. The Future of the Danish Army

    DTIC Science & Technology

    2013-03-01

    as a natural consequence of being a co-founder of the United Nations, focused on promoting peace and stability in the world, as a relatively large...Soviet invasion to a more expeditionary course of deploying forces to promote peace and stability around the globe. As a result, Danish defense policy...Danish government including the armed forces. As a consequence Defense Agreement 2010 – 2014 was replaced by Defense Agreement 2013 – 2017 including

  16. A note comparing the welfare of Zebu cattle following three stunning-slaughter methods.

    PubMed

    Neves, J E G; Paranhos da Costa, M J R; Roça, R O; Faucitano, L; Gregory, N G

    2016-07-01

    The objective of this study was to assess welfare of cattle during bleeding after slaughter with or without stunning. A total of 434 bulls were distributed across three slaughter treatments: penetrating captive bolt stunning followed by chest sticking (PCB, N=279), non-penetrating captive bolt stunning followed by halal slaughter (NPCB, N=67) and shechita without previous stunning (SHE, N=88). Four measures of possible consciousness and return to sensibility were recorded 20 and 60 s after bleeding as welfare indicators. They were the frequencies of responses to nostril stimulation and tongue pinch, spontaneous eye blinking, and rhythmic breathing. All responses were absent in stunned cattle at both 20 and 60 s, and in SHE cattle 7, 4, 10, and 100% of the animals presented these responses, respectively. Repeat shots were required for 46% NPCB and 2% PCB (P<0.05). The application of religious slaughter without previous stunning may result in greater risk of cattle suffering, pain and distress at slaughter.

  17. Abattoir based survey of Salmonella in finishing pigs in the United Kingdom 2006-2007.

    PubMed

    Marier, E A; Snow, L C; Floyd, T; McLaren, I M; Bianchini, J; Cook, A J C; Davies, R H

    2014-12-01

    All European Union (EU) Member States (MSs) were required to conduct a baseline survey from October 2006 to September 2007 to estimate the prevalence of Salmonella infection in finisher pigs at slaughter. In the United Kingdom (UK), samples for microbiological culture were collected from the ileo-caecal lymph nodes, from carcass swabs and from caecal contents. Meat juice samples were also collected for testing in an enzyme-linked immunosorbent assay (MJE) to detect a serological response. Salmonella was isolated from 22% (CI95% 19-25%) of ileo-caecal lymph nodes, 15% (CI95% 12-18%) of carcass swabs and 22% (CI95% 19-26%) of caecal contents. A quarter (25%, CI95% 22-29%) of MJE samples were positive for Salmonella antibodies (cut-off, S/P≥0.25). The most frequently identified serovars were Salmonella typhimurium (57%) and Salmonella derby (26%). The results were used to investigate some factors associated with Salmonella infection in slaughter pigs. The weight of the carcass was significant, with a lower probability of obtaining a positive meat juice result from pigs with a weight greater than 75 kg (p=0.03). The weight of the lymph node sample was significantly associated with Salmonella status, with a heavier sample of lymph nodes being more likely to be positive (OR=2.16 CI95% 1.07-4.39). 'Carcass weight' in two of the classes - 75-79 kg and 80-84 kg - (OR=0.44 CI95% 0.28-0.70; OR=0.64 CI95% 0.49-0.85) and 'fewer-than-1500-pigs' scheduled for slaughter on the day of sampling' (OR=0.41 CI95% 0.24-0.71) were also associated with a reduced risk of isolating Salmonella from lymph node. The model for carcass swabs showed a positive association with 'time-elapsed-since-the-start-of-the-line' indicating a higher risk of contamination as the day progressed (p<0.01). This model also showed positive association between isolation of Salmonella from a carcass swab and the occurrence of Salmonella in caecal contents from the same pig (OR=2.22 CI95% 1.38-3.59) and a negative

  18. Direct contact and environmental contaminations are responsible for HEV transmission in pigs.

    PubMed

    Andraud, Mathieu; Dumarest, Marine; Cariolet, Roland; Aylaj, Bouchra; Barnaud, Elodie; Eono, Florent; Pavio, Nicole; Rose, Nicolas

    2013-10-28

    Hepatitis E virus (HEV) can cause enterically-transmitted hepatitis in humans. The zoonotic nature of Hepatitis E infections has been established in industrialized areas and domestic pigs are considered as the main reservoir. The dynamics of transmission in pig herds therefore needs to be understood to reduce the prevalence of viremic pigs at slaughter and prevent contaminated pig products from entering the food chain. An experimental trial was carried out to study the main characteristics of HEV transmission between orally inoculated pigs and naïve animals. A mathematical model was used to investigate three transmission routes, namely direct contact between pigs and two environmental components to represent within-and between-group oro-fecal transmission. A large inter-individual variability was observed in response to infection with an average latent period lasting 6.9 days (5.8; 7.9) in inoculated animals and an average infectious period of 9.7 days (8.2; 11.2). Our results show that direct transmission alone, with a partial reproduction number of 1.41 (0.21; 3.02), can be considered as a factor of persistence of infection within a population. However, the quantity of virus present in the environment was found to play an essential role in the transmission process strongly influencing the probability of infection with a within pen transmission rate estimated to 2 · 10(-6)g ge(-1)d(-1)(1 · 10(-7); 7 · 10(-6)). Between-pen environmental transmission occurred to a lesser extent (transmission rate: 7 · 10(-8)g ge(-1) d(-1)(5 · 10(-9); 3 · 10(-7)) but could further generate a within-group process. The combination of these transmission routes could explain the persistence and high prevalence of HEV in pig populations.

  19. Occurrence of Toxoplasma gondii in Carcasses of Pigs Reared in Intensive Systems in Northern Italy.

    PubMed

    Papini, Roberto; di Ciccio, Pierluigi; Marangi, Marianna; Ghidini, Sergio; Zanardi, Emanuela; Vergara, Alberto; Giangaspero, Annunziata; Nardoni, Simona; Rocchigiani, Guido; Mancianti, Francesca; Ianieri, Adriana

    2017-03-01

    To evaluate the occurrence of Toxoplasma gondii and to genetically characterize its isolates in carcasses of industrial fattening pigs, blood, diaphragm, and heart samples were collected from 375 carcasses of pigs slaughtered to be processed for Parma ham production. Pigs had been bred on approved farms (n = 75) located in the so-called Food Valley in Italy. Sera were examined for immunoglobulin G antibodies to T. gondii by modified agglutination test (MAT). Both heart and diaphragm samples from seropositive carcasses were processed for the presence of T. gondii DNA (B1 locus) by real-time PCR and high resolution melting (HRM) assay. Anti-Toxoplasma antibodies were detected in 2.1% of pig carcasses, with titers from 1:10 to 1:320. T. gondii DNA was detected in all (eight) seropositive carcasses and in 11 (5 heart and 6 diaphragm samples) of 16 samples; that is, it was detected in heart tissue in two subjects, in diaphragm tissue in three subjects, and in both muscle tissues in three subjects. Toxoplasma genotypes were determined in seven of eight carcasses: type III was identified in four carcasses, type II in two, and both III and II in one carcass. The serological findings and the molecular detection of T. gondii strains suggest that cured meat products obtained from industrially bred pigs may be potential sources of toxoplasmosis for humans. Our results provide novel, important information regarding the seroprevalence and molecular prevalence of T. gondii in intensively reared pigs within this specific region of Italy, particularly because Parma ham from this region is known and consumed worldwide. On-farm preventive measures combined with slaughterhouse monitoring of carcasses of pigs bred for cured meat production should never be overlooked to prevent the introduction of T. gondii into the food chain and to ensure safety for consumers of these products.

  20. Effect of feeding sodium butyrate in the late finishing period on Salmonella carriage, seroprevalence, and growth of finishing pigs.

    PubMed

    Walia, Kavita; Argüello, Hector; Lynch, Helen; Leonard, Finola C; Grant, Jim; Yearsley, Dermot; Kelly, Sinead; Duffy, Geraldine; Gardiner, Gillian E; Lawlor, Peadar G

    2016-09-01

    Pork is an important source of human salmonellosis and low-cost on-farm control measures may provide a useful element in reducing the prevalence of this pathogen in food. This study investigated the effectiveness of dietary supplementation with sodium butyrate administered to finisher pigs for ∼4-weeks prior to slaughter to control Salmonella shedding on highly contaminated farms. Two trials (A and B) were conducted on two commercial pig farms, which had a history of high Salmonella seroprevalence. In both trials, pens (14 pens of 12 pigs/pen in Trial A and 12 pens of 12-17 pigs/pen in Trial B) were randomly assigned to a control (finisher feed without additive) or a treatment group (the same feed with 3kg sodium butyrate/t) for 24-28days, depending on the trial. Faeces were collected from each pig on days 0, 12 and 24/28, and blood, caecal digesta and ileocaecal/mesenteric lymph nodes were collected from the slaughterhouse. Pigs were weighed at the start and end of the trials, feed intake was recorded, and carcass quality parameters were recorded at slaughter. In Trial A, Salmonella shedding was reduced in the treatment compared to the control group at the end of the trial (30% versus 57% probability of detecting Salmonella in faeces, respectively; p<0.001). This reflected the serology results, with detection of a lower seroprevalence in the treatment compared to the control group using the 20% optical density cut-off (69.5% versus 89%; p=0.001). However, no effect on faecal shedding or seroprevalance was observed in Trial B, which may be explained by the detection of a concomitant infection with Lawsonia intracellularis. No significant differences in Salmonella recovery rates were observed in the caecal digesta or lymph nodes in either trial. Furthermore, feed intake, weight gain, and feed conversion efficiency (FCE) did not differ between groups (p>0.05) in either trial. Numerical improvements in weight gain and FCE were found with sodium butyrate treatment

  1. Rearing system and oleic acid supplementation effect on carcass and lipid characteristics of two muscles from an obese pig breed.

    PubMed

    Martins, J M; Neves, J A; Freitas, A; Tirapicos, J L

    2015-10-01

    Quality of pork depends on genotype, rearing and pre- and post-slaughter conditions. However, no information is available on rearing system changes and oleic acid supplementation on carcass characteristics and fatty acid (FA) profile of pork from the Alentejano (AL) pig, an obese breed. This study evaluates the effects of feeding low (LO) or high oleic acid diets (HO) to AL pigs reared in individual pens (IND) or outdoor (OUT) with access to pasture. Carcass composition was obtained and longissimus dorsi and semimembranosus samples were collected to analyse chemical composition and neutral and polar intramuscular lipids FA profile by gas chromatography. Statistical analysis was performed by a two-way ANOVA for rearing system and diet effects. OUT-reared pigs presented leaner carcasses than IND-reared ones. Both muscles presented lower intramuscular lipid content in OUT-reared pigs. Treatments affected the FA profile of muscles. Overall, OUT-reared pigs presented lower n-6/n-3 FA ratios, whereas pigs fed the HO diet exhibited lower saturated fatty acids (SFA), higher monounsaturated fatty acids (MUFA) levels and lower thrombogenic indexes on neutral intramuscular lipids than LO-fed pigs. On the polar fraction, OUT-reared pigs presented lower SAT and n-6/n-3 FA ratio, and higher polyunsaturated fatty acids (PUFA) levels on both muscles. Pigs fed the HO diet exhibited higher MUFA and lower PUFA levels on both muscles, and lower SAT levels on semimembranosus. This study shows rearing system and oleic acid supplementation have complementary effects and influence carcass composition and the nutritional quality of meat.

  2. Assessing the Effectiveness of On-Farm and Abattoir Interventions in Reducing Pig Meat-Borne Salmonellosis within E.U. Member States.

    PubMed

    Hill, Andrew A; Simons, Robin L; Swart, Arno N; Kelly, Louise; Hald, Tine; Snary, Emma L

    2016-03-01

    As part of the evidence base for the development of national control plans for Salmonella spp. in pigs for E.U. Member States, a quantitative microbiological risk assessment was funded to support the scientific opinion required by the EC from the European Food Safety Authority. The main aim of the risk assessment was to assess the effectiveness of interventions implemented on-farm and at the abattoir in reducing human cases of pig meat-borne salmonellosis, and how the effects of these interventions may vary across E.U. Member States. Two case study Member States have been chosen to assess the effect of the interventions investigated. Reducing both breeding herd and slaughter pig prevalence were effective in achieving reductions in the number of expected human illnesses in both case study Member States. However, there is scarce evidence to suggest which specific on-farm interventions could achieve consistent reductions in either breeding herd or slaughter pig prevalence. Hypothetical reductions in feed contamination rates were important in reducing slaughter pig prevalence for the case study Member State where prevalence of infection was already low, but not for the high-prevalence case study. The most significant reductions were achieved by a 1- or 2-log decrease of Salmonella contamination of the carcass post-evisceration; a 1-log decrease in average contamination produced a 90% reduction in human illness. The intervention analyses suggest that abattoir intervention may be the most effective way to reduce human exposure to Salmonella spp. However, a combined farm/abattoir approach would likely have cumulative benefits. On-farm intervention is probably most effective at the breeding-herd level for high-prevalence Member States; once infection in the breeding herd has been reduced to a low enough level, then feed and biosecurity measures would become increasingly more effective.

  3. Carriage and Fecal Counts of Cefotaxime M-Producing Escherichia coli in Pigs: a Longitudinal Study

    PubMed Central

    Damborg, Peter; Andreasen, Margit; Nielsen, Søren Saxmose; Guardabassi, Luca

    2013-01-01

    Current knowledge on extended-spectrum beta-lactamases (ESBLs) in animals is based largely on cross-sectional studies and qualitative data. The aim of this longitudinal study was to elucidate carriage proportions and fecal counts of ESBL-producing Escherichia coli in pigs during the production cycle. At each of three ESBL-positive single-sited farrow-to-finisher pig farms (farms A, B, and C) included in the study, individual fecal samples were taken from 17 to 20 sows 1 week before farrowing and from 2 piglets of each sow's litter four times from birth to slaughter (as piglets, weaners, and finishers). Cefotaxime (CTX)-resistant coliforms in feces were counted on MacConkey agar containing 2 μg/ml CTX and characterized for the presence of ESBL-encoding genes by PCR and sequencing. CTX-M-positive pigs were detected in all age groups at farms A (blaCTX-M-9 group, compatible with blaCTX-M-14/17) and B (blaCTX-M-1 group, compatible with blaCTX-M-1/61), whereas only three weaners were positive at farm C (blaCTX-M-1 group, compatible with blaCTX-M-1/61). A significant decrease in carriage was detected during the production cycle, with on average 50% carriage immediately after birth, 58% just before weaning, 29% during weaning, and 12% during finishing. The observed reduction in numbers of CTX-M-positive pigs was accompanied by a significant reduction in mean fecal counts of CTX-resistant coliforms from ∼107 CFU/g in piglets to ∼103 CFU/g in finishers (P < 0.001). These findings provide novel information about the epidemiology of ESBLs at the farm level and have important implications for assessments of risks of meat contamination during slaughter. PMID:23160131

  4. Effects of immunological castration and distiller's dried grains with solubles on carcass cutability and commercial bacon slicing yields of barrows slaughtered at two time points.

    PubMed

    Tavárez, M A; Bohrer, B M; Asmus, M D; Schroeder, A L; Matulis, R J; Boler, D D; Dilger, A C

    2014-07-01

    Male pigs were randomly assigned to a castration method at birth and allotted to 48 pens (28 pigs/pen). Physically castrated (PC) barrows were castrated at 2 d of age; immunologically castrated (IC) barrows were administered Improvest (GnRF analog diphtheria toxoid conjugate; Zoetis, Kalamazoo, MI) at 16 and 20 wk of age. Distiller's dried grains with solubles (DDGS) feeding strategies included either 0% DDGS (control), 30% DDGS (30% DDGS) fed from 6 wk of age to slaughter, or 30% DDGS fed from 6 wk of age to second dose of Improvest and then fed 0% DDGS until slaughter (withdrawal). Four barrows closest to the median pen weight at 4.5 wk after second dose were selected for evaluation; two were randomly selected and slaughtered at 5 wk and the other two at 7 wk after second dose. Data from each slaughter time were analyzed independently as a 2 × 3 factorial design with pen as the experimental unit. At 5 wk after second dose, bone-in lean cutting yields were 2.63% units greater (P < 0.01) in IC when compared to PC. Bellies were thicker (P < 0.01) and tended to have greater belly flop distances (P = 0.07) in PC compared to IC, however iodine values (IV) were not altered (P = 0.84). Carcass traits (P ≥ 0.10), cutting yields (P ≥ 0.43), and fresh belly characteristics (P ≥ 0.08) were minimally affected by DDGS feeding strategy. Bacon slicing yields (percentage of green weight) were 6.10% units less (P < 0.01) in IC compared with PC. At 7 wk after second dose, bone-in lean cutting yields were 1.57% units greater (P = 0.03) in IC compared with PC. Distiller's grains feeding strategy had no effect (P ≥ 0.83) on boneless carcass cutting yields in IC; while in PC, these yields were 2.32% units less (P < 0.02) in control-fed barrows when compared to other feeding strategies (castration method × feeding strategy; P = 0.03). Bellies from PC tended to be thicker (P = 0.07) and have similar flop distances (P = 0.44) and IV (P = 0.54) when compared with IC. Iodine value

  5. Helminth parasites in pigs: new challenges in pig production and current research highlights.

    PubMed

    Roepstorff, A; Mejer, H; Nejsum, P; Thamsborg, S M

    2011-08-04

    Helminths in pigs have generally received little attention from veterinary parasitologists, despite Ascaris suum, Trichuris suis, and Oesophagostomum sp. being common worldwide. The present paper presents challenges and current research highlights connected with these parasites. In Danish swine herds, new indoor production systems may favour helminth transmission and growing knowledge on pasture survival and infectivity of A. suum and T. suis eggs indicates that they may constitute a serious threat to outdoor pig production. Furthermore, it is now evident that A. suum is zoonotic and the same may be true for T. suis. With these 'new' challenges and the economic impact of the infections, further research is warranted. Better understanding of host-parasite relationships and A. suum and T. suis egg ecology may also improve the understanding and control of human A. lumbricoides and T. trichiura infections. The population dynamics of the three parasites are well documented and may be used to study phenomena, such as predisposition and worm aggregation. Furthermore, better methods to recover larvae have provided tools for quantifying parasite transmission. Thus, an on-going study using helminth naïve tracer pigs has surprisingly demonstrated that soil infectivity with A. suum and T. suis increases during the first 2-3 years after pasture contamination. Though all three helminth species stimulate the Th2 arm of the immune system, Oesophagostomum seems weakly immunogenic, perhaps via specific modulation of the host immune system. A. suum and T. suis potently modulate the host immune response, up-regulating Th2 and down-regulating Th1. As a consequence, A. suum may compromise the efficacy of certain bacterial vaccines, whereas T. suis, which establish only short-term in humans, is a favourite candidate for down-regulating autoimmune Th1-related diseases in man. Some basic research findings have offered new possibilities for future sustainable control measures. For example

  6. Process mapping the prevalence of Salmonella contamination on pork carcass from slaughter to chilling: a systematic review approach.

    PubMed

    O'Connor, Annette M; Wang, Bing; Denagamage, Thomas; McKean, James

    2012-05-01

    A systematic review was conducted to identify and summarize primary research studies that describe the prevalence of Salmonella spp. in pork from slaughter to cooler in the member states of the European Union (EU), Australia, Canada, Hong Kong, Japan, Korea, Mexico, New Zealand, Taiwan, and United States (i.e., a process map). Relevant studies documented Salmonella spp. prevalence at more than one processing point using the same cohort of pigs or the same production line for the post-cooler component. Literature searches retrieved 6811 citations. Sixteen publications, describing 44 studies, evaluated the presence of Salmonella on pork carcasses. The carcass sampling points evaluated were as follows: stun, bleed, kill, scald, dehair, singe, polish, bung removal, evisceration, split, stamp, final wash, immediately after chill, and 18-48 h after chilling. Seventy-eight comparisons of Salmonella spp. prevalence between points along the processing line were reported. The median prevalence of Salmonella spp.-positive carcasses evaluated in the cooler was 0%. The median prevalence of Salmonella spp. after bleeding was 32%. Fifty-nine of the 78 point-to-point comparisons were associated with either no change or a decrease in Salmonella prevalence as the carcass moved closer to the cooler. Nineteen point-to-point changes showed an increase in Salmonella prevalence as the carcass moved toward the cooler; of these, six reported a greater than 10% increase in Salmonella prevalence. The majority of increases were associated with post-evisceration and splitting. These findings suggest that the processing procedures in place generally result in decreased prevalence of Salmonella spp. as the carcasses move toward the cooler.

  7. Fecal carriage of extended-spectrum β-lactamase-producing Enterobacteriaceae in swine and cattle at slaughter in Switzerland.

    PubMed

    Geser, N; Stephan, R; Kuhnert, P; Zbinden, R; Kaeppeli, U; Cernela, N; Haechler, H

    2011-03-01

    During the past decade, extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae have become a matter of great concern in human medicine. ESBL-producing strains are found in the community, not just in hospital-associated patients, which raises a question about possible reservoirs. Recent studies describe the occurrence of ESBL-producing Enterobacteriaceae in meat, fish, and raw milk; therefore, the impact of food animals as reservoirs for and disseminators of such strains into the food production chain must be assessed. In this pilot study, fecal samples of 59 pigs and 64 cattle were investigated to determine the occurrence of ESBL-producing Enterobacteriaceae in farm animals at slaughter in Switzerland. Presumptive-positive colonies on Brilliance ESBL agar were subjected to identification and antibiotic susceptibility testing including the disc diffusion method and E-test ESBL strips. As many as 15.2% of the porcine and 17.1% of the bovine samples, predominantly from calves, yielded ESBL producers. Of the 21 isolated strains, 20 were Escherichia coli, and one was Citrobacter youngae. PCR analysis revealed that 18 strains including C. youngae produced CTX-M group 1 ESBLs, and three strains carried genes encoding for CTX-M group 9 enzymes. In addition, eight isolates were PCR positive for TEM β-lactamase, but no bla(SHV) genes were detected. Pulsed-field gel electrophoresis showed a high genetic diversity within the strains. The relatively high rates of occurrence of ESBLproducing strains in food animals and the high genetic diversity among these strains indicate that there is an established reservoir of these organisms in farm animals. Further studies are necessary to assess future trends.

  8. [Characterization of enteropathogenic Escherichia coli (EPEC) strains isolated during the chicken slaughtering process].

    PubMed

    Alonso, Mónica Z; Sanz, Marcelo E; Padola, Nora L; Lucchesi, Paula M A

    2014-01-01

    In Argentina, EPEC is one of the most prevalent agents isolated from children with diarrhea. Because contamination with this pathotype could occur during slaughter, the aim of this study was to isolate and characterize EPEC strains obtained from live animals (cloacae), eviscerated carcasses, washed carcasses and water from chillers. Twenty nine isolates of atypical EPEC were characterized. These isolates presented a wide variety of serotypes, some of which (O2:H40, O8:H19 and O108:H9) had been reported in other animal species. Serotype O45:H8, previously isolated from children with diarrhea was also found. Isolates of serotypes O2:H40, O108:H9 and O123:H32 were detected at different stages of the slaughtering process, suggesting that the process is not adequately performed. This latter fact highlights the importance of reinforcing control and hygienic measures at different stages of the chicken slaughtering process in order to reduce microbial contamination.

  9. Influence of gas stunning and halal slaughter (no stunning) on rabbits welfare indicators and meat quality.

    PubMed

    Nakyinsige, K; Sazili, A Q; Zulkifli, I; Goh, Y M; Abu Bakar, F; Sabow, A B

    2014-12-01

    This study assessed the effect of gas stunning which has not been conducted until now in comparison with slaughter without stunning on the welfare and meat quality of rabbits. Eighty male New Zealand White rabbits were divided into two groups of 40 animals and subjected to either halal slaughter without stunning (HS) or gas stunning using 61.4% CO2, 20.3% oxygen and 18.3 % nitrogen (GS). Analysis of the sticking blood revealed that both slaughter procedures caused a substantial increase in the levels of catecholamines, hypercalcemia, hyperglycemia, lactic acidemia and an increase in enzyme activities. The ultimate pH of the Longissimus lumborum muscle did not differ between treatments. GS exhibited higher lightness and cooking loss, and lower glycogen and MFI than HS. This indicates that both GS and HS can be significant stressors although the amount of stress may be below the threshold to negatively affect rabbit meat quality.

  10. Foodborne zoonoses due to meat: a quantitative approach for a comparative risk assessment applied to pig slaughtering in Europe.

    PubMed

    Fosse, Julien; Seegers, Henri; Magras, Catherine

    2008-01-01

    Foodborne zoonoses have a major health impact in industrialised countries. New European food safety regulations were issued to apply risk analysis to the food chain. The severity of foodborne zoonoses and the exposure of humans to biological hazards transmitted by food must be assessed. For meat, inspection at the slaughterhouse is historically the main means of control to protect consumers. However, the levels of detection of biological hazards during meat inspection have not been established in quantitative terms yet. Pork is the most frequently consumed meat in Europe. The aim of this study was to provide elements for quantifying levels of risk for pork consumers and lack of detection by meat inspection. Information concerning hazard identification and characterisation was obtained by the compilation and statistical analysis of data from 440 literature references. The incidence and severity of human cases due to pork consumption in Europe were assessed in order to calculate risk scores. A ratio of non-control was calculated for each biological hazard identified as currently established in Europe, i.e. the incidence of human cases divided by the prevalence of hazards on pork. Salmonella enterica, Yersinia enterocolitica and Campylobacter spp. were characterised by high incidence rates. Listeria monocytogenes, Clostridium botulinum and Mycobacterium spp. showed the highest severity scores. The three main high risk hazards involved in foodborne infections, Y. enterocolitica, S. enterica and Campylobacter spp. are characterised by high non-control ratios and cannot be detected by macroscopic examination of carcasses. New means of hazard control are needed to complement the classical macroscopic examination.

  11. Urolithiasis in finishing pigs.

    PubMed

    Maes, D G D; Vrielinck, J; Millet, S; Janssens, G P J; Deprez, P

    2004-11-01

    Urolithiasis in sows and neonatal pigs is well-known, but information on its occurrence and impact in finishing pigs is sparse. This study reports three outbreaks of urolithiasis in finishing pigs. In one herd, no symptoms were observed, whereas in the other herds the presence of calculi caused obstruction of the urinary tract resulting in death. Using infra-red spectroscopy, the predominant mineral-type found in the uroliths was calcium carbonate (calcite). Only small amounts of calcium oxalate (< 1%) could be detected. A high urinary pH, small abnormalities in the mineral composition of the feed and insufficient drinking water were the most important risk factors identified. To prevent urolithiasis, it is important to ensure adequate water intake, to provide a balanced mineral diet, and to avoid urinary tract infections.

  12. Comparative carcass and tissue nutrient composition of transgenic Yorkshire pigs expressing phytase in the saliva and conventional Yorkshire pigs.

    PubMed

    Forsberg, C W; Meidinger, R G; Ajakaiye, A; Murray, D; Fan, M Z; Mandell, I B; Phillips, J P

    2014-10-01

    A transgenic line of Yorkshire (YK) pigs named the Cassie (CA) line was produced with a low copy number phytase transgene inserted in the genome. The transgenic line efficiently digests P, Ca, and other major minerals of plant dietary origin. The objectives of this study were to 1) compare carcass and tissue nutrient composition and meat quality traits for third generation hemizygous CA line market BW finisher pigs (n = 24) with age-matched conventional YK finisher pigs (n = 24) and 2) examine effects of outbreeding with high-index conventional YK boars on modifying carcass leanness from the third to sixth generations in CA line finisher boars (n = 73) and gilts (n = 103). Cassie boars (n = 12) and CA gilts (n = 12) were fed diets without supplemental P and comparable numbers of age-matched YK boars and gilts fed diets containing supplement P were raised throughout the finisher phase. The pigs were slaughtered and then fabricated into commercial pork primals before meat composition and quality evaluation. Proximate and major micronutrient composition was determined on tissues including fat, kidney, lean, liver, and skin. The main difference observed was greater (P = 0.033) crude fat content in CA boar carcasses and increased (P < 0.04) leaf lard in both CA boars and gilts but no differences were observed (P = 0.895 and P = 0.223, respectively) in carcass backfat thickness as compared with YK pigs. There were no substantive differences in tissue composition, except for CA boar kidneys. Numerous changes in the mineral, fatty acid, and indispensable AA composition for CA boar kidneys were not apparent in CA gilts. These changes may point to adaptive physiological changes in the boar kidney necessary for homeostatic regulation of mineral retention related to phytase action rather than to insertion of the transgene. However, from a meat composition perspective, transgenic expression of phytase in the CA line of YK pigs had little overall effect on meat composition

  13. Assessment of bacterial superficial contamination in classical or ritually slaughtered cattle using metagenetics and microbiological analysis.

    PubMed

    Korsak, N; Taminiau, B; Hupperts, C; Delhalle, L; Nezer, C; Delcenserie, V; Daube, G

    2017-04-17

    The aim of this study was to investigate the influence of the slaughter technique (Halal vs Classical slaughter) on the superficial contamination of cattle carcasses, by using traditional microbiological procedures and 16S rDNA metagenetics. The purpose was also to investigate the neck area to identify bacteria originating from the digestive or the respiratory tract. Twenty bovine carcasses (10 from each group) were swabbed at the slaughterhouse, where both slaughtering methods are practiced. Two swabbing areas were chosen: one "legal" zone of 1600cm(2) (composed of zones from rump, flank, brisket and forelimb) and locally on the neck area (200cm(2)). Samples were submitted to classical microbiology for aerobic Total Viable Counts (TVC) at 30°C and Enterobacteriaceae counts, while metagenetic analysis was performed on the same samples. The classical microbiological results revealed no significant differences between both slaughtering practices; with values between 3.95 and 4.87log CFU/100cm(2) and 0.49 and 1.94log CFU/100cm(2), for TVC and Enterobacteriaceae respectively. Analysis of pyrosequencing data showed that differences in the bacterial population abundance between slaughtering methods were mainly observed in the "legal" swabbing zone compared to the neck area. Bacterial genera belonging to the Actinobacteria phylum were more abundant in the "legal" swabbing zone in "Halal" samples, while Brevibacterium and Corynebacterium were encountered more in "Halal" samples, in all swabbing areas. This was also the case for Firmicutes bacterial populations (families of Aerococcaceae, Planococcaceae). Except for Planococcoceae, the analysis of Operational Taxonomic Unit (OTU) abundances of bacteria from the digestive or respiratory tract revealed no differences between groups. In conclusion, the slaughtering method does not influence the superficial microbiological pattern in terms of specific microbiological markers of the digestive or respiratory tract. However

  14. Segmental Arterial Mediolysis in Pigs Presenting With Renal Infarcts.

    PubMed

    Leifsson, P S; Slavin, R E

    2015-11-01

    Segmental arterial mediolysis, a rare arteriopathy first reported in humans, is described in the kidneys of 36 pigs slaughtered in an abattoir in Jutland, Denmark. The kidney changes presented themselves macroscopically as one or more cortical wedge-shaped hemorrhagic or pale lesions. The arterial lesions involved the interlobar and arcuate arteries and exhibited injurious and reparative phases of development. Two types of injurious lesions occurred: (1) a tearing separation of the outer media from the adventitia with fibrin, erythrocytes, and edema fluid filling the formed space, causing collapse of the arterial wall, and (2) outer and mid-medial foci showing irregularly bordered cytoplasmic vacuolar change containing membranous and organelle debris or smooth muscle shrinkage with nuclear loss. In the reparative phase, granulation tissue filled and expanded tear sites and zones of arterial medial muscle loss and extended into the adventitia and through the intima into the arterial lumen. Sequelae, including dissecting hematomas and arterial occlusions causing renal infarcts, were found. Although repartitioning agents widely used in animal husbandry in many countries may potentially cause segmental arterial mediolysis, no such link could be identified. The causation of segmental arterial mediolysis in these pigs is currently unknown but is being further investigated.

  15. PCR test for detecting Taenia solium cysticercosis in pig carcasses.

    PubMed

    Sreedevi, Chennuru; Hafeez, Mohammad; Kumar, Putcha Anand; Rayulu, Vukka Chengalva; Subramanyam, Kothapalli Venkata; Sudhakar, Krovvidi

    2012-01-01

    Polymerase chain reaction (PCR) test was employed to detect Taenia solium DNA in muscle lesions for validation of the meat inspection results of slaughtered pigs. Two sets of oligonucleotide primers, one targeted against the large subunit rRNA gene (TBR primers) and the other targeted against cytochrome c oxidase subunit 1 gene (Cox1 primers) of T. solium were used in this study. On reactivity in PCR test, the TBR primers and the Cox1 primers yielded products of 286 and 984 bp, respectively, in cysticercosis positive cases. Both the sets of primers were found to be highly specific, since they did not yield any PCR product in negative controls. A total of 225 pig carcasses were screened for cysticercosis by meat inspection, out of which 25 carcasses with visible cysts (16 viable and 9 degenerated cysts) were also confirmed to be positive for cysticercosis in PCR test. However, out of the 35 carcasses with suspected lesions on meat inspection, only two were found to be positive for cysticercosis in PCR test. The detection limits for both the primer sets were analyzed. The TBR primer set could detect up to 10 pg of cysticercus DNA, whereas the Cox1 primer set could detect only up to 1 ng. It is evident from the study that PCR test is an efficient tool for validation of meat inspection results and also to rule out ambiguity in carcass judgment of suspected cases of porcine cysticercosis.

  16. Danish Breast Cancer Cooperative Group

    PubMed Central

    Christiansen, Peer; Ejlertsen, Bent; Jensen, Maj-Britt; Mouridsen, Henning

    2016-01-01

    Aim of database Danish Breast Cancer Cooperative Group (DBCG), with an associated database, was introduced as a nationwide multidisciplinary group in 1977 with the ultimate aim to improve the prognosis in breast cancer. Since then, the database has registered women diagnosed with primary invasive nonmetastatic breast cancer. The data reported from the departments to the database included details of the characteristics of the primary tumor, of surgery, radiotherapy, and systemic therapies, and of follow-up reported on specific forms from the departments in question. Descriptive data From 1977 through 2014, ~110,000 patients are registered in the nationwide, clinical database. The completeness has gradually improved to more than 95%. DBCG has continuously prepared evidence-based guidelines on diagnosis and treatment of breast cancer and conducted quality control studies to ascertain the degree of adherence to the guidelines in the different departments. Conclusion Utilizing data from the DBCG database, a long array of high-quality DBCG studies of various designs and scope, nationwide or in international collaboration, have contributed to the current updating of the guidelines, and have been an instrumental resource in the improvement of management and prognosis of breast cancer in Denmark. Thus, since the establishment of DBCG, the prognosis in breast cancer has continuously improved with a decrease in 5-year mortality from ~37% to 15%. PMID:27822082

  17. Effects of Dietary Energy Sources on Post Mortem Glycolysis, Meat Quality and Muscle Fibre Type Transformation of Finishing Pigs.

    PubMed

    Li, Yanjiao; Li, Jiaolong; Zhang, Lin; Yu, Changning; Lin, Meng; Gao, Feng; Zhou, Guanghong; Zhang, Yu; Fan, Yuanfang; Nuldnali, Lina

    2015-01-01

    Dietary energy source can influence muscle glycogen storage at slaughter. However, few studies have demonstrated whether the diet-induced change of muscle glycogen is achieved by the transformation of muscle fibre type. This study investigated the effects of dietary energy sources on meat quality, post mortem glycolysis and muscle fibre type transformation of finishing pigs. Seventy-two barrows with an average body weight of 65.0 ± 2.0 kg were selected and were allotted to three iso-energetic and iso-nitrogenous diets A, B or C, and each treatment consisted of three replicates (pens) of eight pigs each. Diet A contained 44.1% starch, 5.9% crude fat and 12.6% neutral detergent fiber (NDF); diet B contained 37.6% starch, 9.5% crude fat and 15.4% NDF; and diet C contained 30.9% starch, 14.3% crude fat and 17.8% NDF. The duration of the experiment was 28 days. After feed withdrawal 12 h, 24 pigs (eight per treatment) were slaughtered, samples from M. longissimus lumborum (LL) were collected for subsequent analysis. The results showed that pigs fed diet C had lesser average daily gain, average daily feed intake and back fat depth than those fed diet A (P<0.05). Diet C increased pH45min (P<0.05) and decreased drip loss (P<0.05) in LL muscles compared with diet A. Meat from pigs fed diet A showed increased contents of lactate and greater glycolytic potential (GP) compared with those fed diet C (P<0.05). Greater mRNA expression of myosin heavy-chain (MyHC)-I and IIa and lesser expression of MyHC-IIx and IIb (P<0.05) in LL muscles were found in pigs fed diet C, than in pigs fed diet A. In addition, pigs fed diet C resulted in downregulation of miR23a and upregulation of miR409 and miR208b (P<0.05), associated with conserved changes of their corresponding targets. These findings indicated that diets containing low starch and high fibre were beneficial in reducing muscle glycolysis, improving meat quality of finishing pigs. This reduction of GP may be partially associated

  18. Effects of Dietary Energy Sources on Post Mortem Glycolysis, Meat Quality and Muscle Fibre Type Transformation of Finishing Pigs

    PubMed Central

    Li, Yanjiao; Li, Jiaolong; Zhang, Lin; Yu, Changning; Lin, Meng; Gao, Feng; Zhou, Guanghong; Zhang, Yu; Fan, Yuanfang; Nuldnali, Lina

    2015-01-01

    Dietary energy source can influence muscle glycogen storage at slaughter. However, few studies have demonstrated whether the diet-induced change of muscle glycogen is achieved by the transformation of muscle fibre type. This study investigated the effects of dietary energy sources on meat quality, post mortem glycolysis and muscle fibre type transformation of finishing pigs. Seventy-two barrows with an average body weight of 65.0 ± 2.0 kg were selected and were allotted to three iso-energetic and iso-nitrogenous diets A, B or C, and each treatment consisted of three replicates (pens) of eight pigs each. Diet A contained 44.1% starch, 5.9% crude fat and 12.6% neutral detergent fiber (NDF); diet B contained 37.6% starch, 9.5% crude fat and 15.4% NDF; and diet C contained 30.9% starch, 14.3% crude fat and 17.8% NDF. The duration of the experiment was 28 days. After feed withdrawal 12 h, 24 pigs (eight per treatment) were slaughtered, samples from M. longissimus lumborum (LL) were collected for subsequent analysis. The results showed that pigs fed diet C had lesser average daily gain, average daily feed intake and back fat depth than those fed diet A (P<0.05). Diet C increased pH45min (P<0.05) and decreased drip loss (P<0.05) in LL muscles compared with diet A. Meat from pigs fed diet A showed increased contents of lactate and greater glycolytic potential (GP) compared with those fed diet C (P<0.05). Greater mRNA expression of myosin heavy-chain (MyHC)-I and IIa and lesser expression of MyHC-IIx and IIb (P<0.05) in LL muscles were found in pigs fed diet C, than in pigs fed diet A. In addition, pigs fed diet C resulted in downregulation of miR23a and upregulation of miR409 and miR208b (P<0.05), associated with conserved changes of their corresponding targets. These findings indicated that diets containing low starch and high fibre were beneficial in reducing muscle glycolysis, improving meat quality of finishing pigs. This reduction of GP may be partially associated

  19. 9 CFR 313.50 - Tagging of equipment, alleyways, pens, or compartments to prevent inhumane slaughter or handling...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 2 2013-01-01 2013-01-01 false Tagging of equipment, alleyways, pens, or compartments to prevent inhumane slaughter or handling in connection with slaughter. 313.50 Section 313.50 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT OF...

  20. 9 CFR 381.67 - Young chicken and squab slaughter inspection rate maximums under traditional inspection procedure.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 2 2014-01-01 2014-01-01 false Young chicken and squab slaughter inspection rate maximums under traditional inspection procedure. 381.67 Section 381.67 Animals and Animal... INSPECTION REGULATIONS Operating Procedures § 381.67 Young chicken and squab slaughter inspection...

  1. 9 CFR 381.67 - Young chicken and squab slaughter inspection rate maximums under traditional inspection procedure.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 2 2012-01-01 2012-01-01 false Young chicken and squab slaughter inspection rate maximums under traditional inspection procedure. 381.67 Section 381.67 Animals and Animal... INSPECTION REGULATIONS Operating Procedures § 381.67 Young chicken and squab slaughter inspection...

  2. 9 CFR 381.67 - Young chicken and squab slaughter inspection rate maximums under traditional inspection procedure.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 2 2011-01-01 2011-01-01 false Young chicken and squab slaughter inspection rate maximums under traditional inspection procedure. 381.67 Section 381.67 Animals and Animal... INSPECTION REGULATIONS Operating Procedures § 381.67 Young chicken and squab slaughter inspection...

  3. 76 FR 26927 - National Organic Program; Notice on the Ruminant Slaughter Stock Provision of the Access to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-10

    ... Service 7 CFR Part 205 National Organic Program; Notice on the Ruminant Slaughter Stock Provision of the... National Organic Program (NOP) to amend the provision on ruminant slaughter stock under the NOP regulations..., National Organic Program, USDA-AMS-NOP, 1400 Independence Ave., SW., Room 2646-So., Ag Stop...

  4. 9 CFR 381.67 - Young chicken and squab slaughter inspection rate maximums under traditional inspection procedure.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 2 2013-01-01 2013-01-01 false Young chicken and squab slaughter... INSPECTION REGULATIONS Operating Procedures § 381.67 Young chicken and squab slaughter inspection rate... inspector per minute under the traditional inspection procedure for the different young chicken and...

  5. 9 CFR 93.420 - Ruminants from Canada for immediate slaughter other than bovines, sheep, and goats.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Ruminants from Canada for immediate... Ruminants Canada 8 § 93.420 Ruminants from Canada for immediate slaughter other than bovines, sheep, and... are contained in § 93.436. All other ruminants imported from Canada for immediate slaughter,...

  6. 9 CFR 93.420 - Ruminants from Canada for immediate slaughter other than bovines, sheep, and goats.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Ruminants from Canada for immediate... Ruminants Canada 8 § 93.420 Ruminants from Canada for immediate slaughter other than bovines, sheep, and... are contained in § 93.436. All other ruminants imported from Canada for immediate slaughter,...

  7. 9 CFR 93.420 - Ruminants from Canada for immediate slaughter other than bovines, sheep, and goats.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Ruminants from Canada for immediate... Ruminants Canada 8 § 93.420 Ruminants from Canada for immediate slaughter other than bovines, sheep, and... are contained in § 93.436. All other ruminants imported from Canada for immediate slaughter,...

  8. 9 CFR 93.420 - Ruminants from Canada for immediate slaughter other than bovines, sheep, and goats.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Ruminants from Canada for immediate... Ruminants Canada 8 § 93.420 Ruminants from Canada for immediate slaughter other than bovines, sheep, and... are contained in § 93.436. All other ruminants imported from Canada for immediate slaughter,...

  9. 9 CFR 93.420 - Ruminants from Canada for immediate slaughter other than bovines, sheep, and goats.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Ruminants from Canada for immediate... Ruminants Canada 8 § 93.420 Ruminants from Canada for immediate slaughter other than bovines, sheep, and... are contained in § 93.436. All other ruminants imported from Canada for immediate slaughter,...

  10. Effects of the level of sugarcane molasses on growth and carcass performance of Caribbean growing pigs reared under a ground sugarcane stalks feeding system.

    PubMed

    Xandé, X; Archimède, H; Gourdine, J L; Anais, C; Renaudeau, D

    2010-01-01

    The influence of the level of sugarcane (SC) molasses on growth performance, carcass traits, and meat quality in Creole (CR) growing pigs fed with ground sugarcane stalks (GCS)-based diet was studied in a mixed farming system context. The aim of the study was to optimize the growth performance of CR pigs with SC-molasses as an energy source in this unconventional feeding. A total of 32 CR pigs were used from 30 to 60 kg of body weight (BW). The experimental dietary treatments consisted of four levels of inclusion of SC-molasses (200, 400, 600, and 800 g DM/d/pig) into a GCS diet, for diets 1, 2, 3 and 4 respectively. The GCS allowance was based on live BW (170 g/kg BW/d) and the diets were supplemented with a soya-bean meal supplement (350 g/d of a 49.2% CP and 16.6 MJ DE/kg). All the pigs were slaughtered at 60 kg BW. Increasing the level of molasses did not affect (p > 0.05) average BW gain (254 g/d), CP intake (154 g/d) and sugar extraction rate from the total ration (85%). A gradual inclusion of molasses in a GCS-based diet did not affect the carcass and meat quality of CR pigs. In conclusion, molasses supplementation does not allow the increase of growth performance in GCS fed pigs.

  11. [Proximal composition, lipid and cholesterol content of meat from pigs fed peach-palm meal (Bactris gasipaes Kunth) and synthetic lysine].

    PubMed

    Jerez-Timaure, Nancy; Rivero, Janeth Colina; Araque, Humberto; Jiménez, Paola; Velazco, Mariela; Colmenares, Ciolys

    2011-03-01

    Two experiments were conducted to evaluate the proximal composition, lipids and cholesterol content of meat from pigs fed diets with peach-palm meal (PPM), with or without addition of synthetic lysine (LYS). In experiment 1, 24 pigs were randomly allotted into six treatments with three levels of PPM (0.16 and 32%) and two levels of LYS (0 and 0.27%). In experiment II, 16 finishing pigs were fed with two levels of PPM (0 and 17.50%) and two levels of LYS (0 and 0.27%). At the end of each experiment (42 and 35 d, respectively), pigs were slaughtered and loin samples were obtained to determine crude protein, dry matter, moisture, ash, total lipids, and cholesterol content. In experiment I, pork loin from 16% PPM had more dry matter (26.45 g/100 g) and less moisture (73.49 g/100g) than pork loin from 32% PPM (25.11 y 75.03 g/100g, respectively). Meat samples from pigs without LYS had higher (p < 0.05) content of lipids (2.11 g/100 g) than meat from pigs that consumed LYS (1.72 g/100 g). In experiment II, the proximal, lipids and cholesterol content were similar among treatments. The PPM addition to pig diets did not affect the proximal composition of pork, while LYS addition indicated a reduction of total lipids, which could result as an alternative to obtain leaner meat.

  12. Prevalence and molecular epidemiology of porcine cysticercosis in naturally infected pigs (Sus scrofa) in Punjab, India.

    PubMed

    Chawhan, P; Singh, B; Sharma, R; Gill, P S

    2015-12-01

    Porcine cysticercosis is a serious zoonosis in resource-poor countries. Despite the evidence showing that the disease is endemic in the Punjab region of India, molecular characterisation of Taenia solium cysticercosis from naturally infected pigs has not been carried out. The authors examined a total of 519 pigs slaughtered in small slaughter shops (shops that sell meat from animals that are slaughtered on the premises as the customer waits) in the urban slums of Punjab state in northern India. The expected polymerase chain reaction products with molecular sizes of 286 bp, 420 bp, 1150 bp and 333 bp corresponding to the targeted large subunit ribosomal RNA (rRNA), cytochrome oxidase 1, internal transcribed spacer 1, and diagnostic antigen Ts14 genes, respectively, were amplified from the cysts collected from all 22 infected carcasses. The detection limits for the respective primers (except those targeting the Ts14 gene) were estimated. The analytical sensitivities of both the TBR and JB primers (targeting the rRNA and cytochrome oxidase genes, respectively) were found to be higher (10 pg) than that of the internal transcribed spacer 1 gene (1 ng) primers. Ten representative samples from cytochrome oxidase 1 gene amplified products were sequenced in both directions for phylogenetic analysis. Sequencing demonstrated that all cysticerci were of the Asian genotype of T. solium and not of the African/Latin American genotype or T. asiatica. The results confirm the presence of T. solium porcine cysticercosis in Punjab state and there is therefore an urgent need for science-based policies for prevention and control of this serious zoonosis.

  13. Feeding dried chicory root to pigs decrease androstenone accumulation in fat by increasing hepatic 3β hydroxysteroid dehydrogenase expression.

    PubMed

    Rasmussen, Martin Krøyer; Brunius, Carl; Zamaratskaia, Galia; Ekstrand, Bo

    2012-05-01

    The present study investigated the in vivo effect of chicory root on testicular steroid concentrations and androstenone metabolizing enzymes in entire male pigs. Furthermore, the effect on skatole and indole concentrations in plasma and adipose tissue was investigated. The pigs were divided into two groups; one receiving experimental feed containing 10% dried chicory root for 16 days before slaughter, the control group was fed a standard diet. Plasma, adipose and liver tissue samples were collected at slaughter. Plasma was analyzed for the concentration of testosterone, estradiol, insulin-like growth factor 1 (IGF-1), skatole and indole. Adipose tissue was analyzed for the concentration of androstenone, skatole and indole, while the liver tissue was analyzed for mRNA and protein expressions of 3β-hydroxysteroid dehydrogenase (3β-HSD), sulfotransferase 2A1 and heat-shock protein 70 (HSP70). The results showed that the androstenone concentrations in the adipose tissue of chicory fed pigs were significantly (p<0.05) lower and indole concentrations were higher (p<0.05) compared to control fed pigs. Moreover the chicory root fed pigs had increased mRNA and protein expression of 3β-HSD and decreased HSP70 expression (p<0.05). Testosterone and IGF-1 concentrations in plasma as well as skatole concentrations in adipose tissue were not altered by dietary intake of chicory root. It is concluded that chicory root in the diet reduces the concentration of androstenone in adipose tissue via induction of 3β-HSD, and that these changes were not due to increased cellular stress.

  14. Effect of drinking-water administration of experimental chlorate ion preparations on Salmonella enterica serovar Typhimurium colonization in weaned and finished pigs.

    PubMed

    Anderson, R C; Hume, M E; Genovese, K J; Callaway, T R; Jung, Y S; Edrington, T S; Poole, T L; Harvey, R B; Bischoff, K M; Nisbet, D J

    2004-04-01

    Foodborne disease caused by Salmonella is of public health and economic significance. In order to assess the practical effectiveness of a new intervention strategy, experimental chlorate preparations (ECP) were administered via the drinking water to weaned and finished pigs that had been orally challenged the previous day with 10(9)-10(10) colony-forming units of Salmonella serovar Typhimurium. After 24 or 36 h ad libitum access to 0X, 1X or 2X ECP treatment (where X is the concentration estimated to deliver a minimal daily effective dose), the pigs were euthanized and gut contents and lymph tissue collected at necropsy were cultured for the challenge Salmonella. Drinking water administration of ECP effectively reduced (p < 0.05) caecal Salmonella concentrations and, with the weaned pigs, tended (p < or = 0.10) to reduce rectal Salmonella concentrations. No negative effects of ECP treatment on water intake and animal wellbeing were observed and only marginal effects on gut fermentation characteristics occurred. The bactericidal effect of administering ECP in drinking water was relatively rapid, with reductions in caecal Salmonella concentrations occurring within 24 h. These results suggest that ECP administered to pigs just days before slaughter may reduce gut concentrations of Salmonella; however, the impacts of such reductions on slaughter hygiene have yet to be determined.

  15. Evaluation of Mycoplasma hyopneumoniae inactivated vaccine in pigs under field conditions.

    PubMed

    Okada, M; Sakano, T; Senna, K; Maruyama, T; Murofushi, J; Okonogi, H; Sato, S

    1999-10-01

    An inactivated vaccine prepared from broth culture supernatant of Mycoplasma hyopneumoniae with an aluminum adjuvant was evaluated in three herds (herd A: specific pathogen-free herd, herd B: high health status herd with no clinical signs of respiratory infection, herd C: low health status herd with serious epidemiological and economical problems). A total of 212 pigs from the three herds were divided into two groups. One group was injected twice with the vaccine at 4-week intervals and the other was a control group. No adverse reactions were noted following the vaccinations either systematically or locally in any of the vaccinated pigs from any of the herds. In herd A, the vaccination provided antibody response within 4 weeks after the second vaccination and antibody responses continued for more than 12 weeks. In herds B and C, the number of pigs with lung lesions, mean percentage of lung lesions, and the numbers of M. hyopneumoniae recovered from pigs at slaughter in the vaccinated group were significantly (P < 0.05) reduced compared to the control group. Furthermore, vaccination resulted in improved average daily weight gain (ADG), improved feed conversion ratio (FCR), and improved days to market weight in herd C, whereas no difference in growth performance was shown in herd B. It is suggested that the inactivated vaccine prepared from broth culture supernatant of M. hyopneumoniae is effective in reducing clinical signs and lung lesions. Also, vaccination resulted in improved growth performance in herds where clinical signs and economic losses were significant.

  16. A Farm Transmission Model for Salmonella in Pigs, Applicable to E.U. Member States.

    PubMed

    Hill, Andrew A; Simons, Robin R L; Kelly, Louise; Snary, Emma L

    2016-03-01

    The burden of Salmonella entering pig slaughterhouses across the European Union is considered a primary food safety concern. To assist E.U. member states with the development of national control plans, we have developed a farm transmission model applicable to all member states. It is an individual-based stochastic susceptible-infected model that takes into account four different sources of infection of pigs (sows, feed, external contaminants such as rodents, and new stock) and various management practices linked to Salmonella transmission/protection (housing, flooring, feed, all-in-all-out production). A novel development within the model is the assessment of dynamic shedding rates. The results of the model, parameterized for two case study member states (one high and one low prevalence) suggest that breeding herd prevalence is a strong indicator of slaughter pig prevalence. Until a member state's' breeding herd prevalence is brought below 10%, the sow will be the dominant source of infection to pigs raised for meat production; below this level of breeding herd prevalence, feed becomes the dominant force of infection.

  17. Phenotypic features of the domestic pigs bred in the Roman settlements of Pompeii and Caralis.

    PubMed

    Manca, Paolo; Farina, Vittorio; Gadau, Sergio; Lepore, Gianluca; Genovese, Angelo; Zedda, Marco

    2004-01-01

    A reconstruction of the morphological features of domestic pigs from two Roman settlements is here suggested by means of the study of skeletal and dental remains, with the aim at evaluating their degree of selection in comparison with wild boars. Material was formed by 111 bone and tooth fragments and was uncovered during the excavations of Polybius' House in Pompeii and of Roman buildings in the neighbourhood of Caralis harbour (Sardinia). The remains underwent morphological examination. The eruption of permanent teeth and ossification of epiphyseal cartilages let us establish that most animals were over 18-20 months. When possible, the determination of sex was made by detecting tusk features. The presence of anthropic signs on the bone surface provides some information about slaughtering and cooking procedure in the Roman period and supports the hypothesis that the animal remnants were food remains. Osteometric analysis was carried out on long and short bones and teeth through suitable multiplicative parameters, leading to the assessment of the withers height and other main phenotypic features. Logarithmic deviation pointed out the significant osteometric differences between the domestic pigs from the two Roman settlements. These data were also compared with those from wild boars and modern crossbred wild boars X non-selected pigs. In conclusion, our data show that pigs from Caralis bear much resemblance to wild boars, whereas those from Pompeii appear to be improved, so sharing some phenotypic features of modem improved breeds.

  18. Carcass characteristics and fat depots in Iberian and F Large White × Landrace pigs intensively finished or raised outdoors in oak-tree forests.

    PubMed

    Bressan, M C; Almeida, J; Santos Silva, J; Bettencourt, C; Francisco, A; Gama, L T

    2016-06-01

    A factorial experiment was performed with 117 barrows belonging to the Iberian (IB) and crossbred F Large White × Landrace (F) genetic groups, either intensively finished (IN) or finished outdoors on pasture in an oak and cork tree forest (EX). Information was collected on carcass weight, yield, and dimensions; weight of organs, carcass cuts, and abdominal fat depots; backfat depth; measurements of the longissimus thoracis (LT); and yield of different leg tissues. For the 41 slaughter and carcass traits analyzed, the interaction between genetic group and finishing system was significant ( < 0.05) in 18 traits, and overall, there was a more pronounced influence of genetic group than of finishing system. In most variables, particularly those related with fat deposition, the interaction reflected mostly changes in mean differences among genetic groups rather than in their ranking, where IB pigs consistently produced fatter carcasses, regardless of the finishing system. Liver weight in IB-EX pigs was lower by nearly 8% when compared with F-EX or IB-IN pigs, but the opposite pattern was found in F pigs, where liver weight in F-EX pigs was higher by 16% relative to IB-EX pigs or to F-IN pigs. The deposition of adipose tissue was much larger ( < 0.05) in IB pigs compared with F pigs, with means for fat depots in IB pigs that were higher by about 25% in total abdominal fat, 94% in dorsal fat depth, 72% in intermuscular plus subcutaneous fat in the leg, and over 300% in intramuscular fat (IMF). The deposition of lean tissue was much lower in IB pigs ( < 0.05), with means for trimmed loin weight corresponding to about one-half of the means obtained in F pigs, whereas lean percentage in the leg of IB pigs was about two-thirds of the mean in F pigs and the mean area of the LT was nearly one-half of that observed in F pigs in the same finishing system ( < 0.05). A strong correlation was observed between the various fat depots when the full data set was considered (correlations

  19. Transportation of market-weight pigs: I. effect of season, truck type, and location within truck on behavior with a two-hour transport.

    PubMed

    Torrey, S; Bergeron, R; Widowski, T; Lewis, N; Crowe, T; Correa, J A; Brown, J; Gonyou, H W; Faucitano, L

    2013-06-01

    There is evidence that season and truck/trailer design play important roles in pig welfare during transportation although little is known about their interaction and effect on pig behavior. This experiment was designed to examine the influence of season and truck/trailer design on the behavior during loading, transit, unloading, and lairage of market-weight pigs transported to slaughter. A total of 3,756 pigs were transported on either a 3-deck pot-belly trailer (PB; n = 181 pigs/wk in 8 experimental compartments) or a double-decker hydraulic truck (DD; n = 85 pigs/wk in 4 compartments) for 2 h to a commercial abattoir in summer and winter (6 wk in each season). Density on both vehicles was 0.40 m(2)/pig. Accounting for the number of pigs, loading took longer (P = 0.033) onto the DD than the PB, but season did not (P = 0.571) influence loading time. Pigs loaded onto the PB moved backward more (P = 0.003) frequently than those loaded onto the DD. The frequency of tapping by handler was the lone handling intervention affected by truck type, with more (P = 0.014) tapping needed to move pigs on and off DD than PB. During loading, pigs made more (P < 0.001) slips and falls, overlaps, 180° turns, underlaps, and vocalizations in winter compared with summer. On truck, more (P < 0.001) pigs were standing on the DD at the farm and in transit than on the PB whereas more (P = 0.012) pigs were lying in transit in summer than in winter. Pigs took longer to unload (P < 0.001) from the PB than the DD, but no difference between vehicles (P = 0.473) in latency to rest in lairage was found. Pigs slipped and fell more (P < 0.001) during unloading, took longer (P < 0.001) to unload, and had a shorter (P = 0.006) latency to rest in lairage in winter than summer. Vehicle design, in particular the presence of ramps, influenced pig behavior before, during, and after transportation, regardless of the season. Season affected loading and unloading behavior, especially in terms of slips and

  20. Influence of a gonadotropin-releasing hormone vaccine and dietary standardized ileal digestible lysine level on growth performance and carcass quality of grower-finisher pigs.

    PubMed

    Molist, F; Gerritsen, R; van der Aar, P; Prüst, H

    2014-11-01

    The aim of this study was to assess the effect of standardized ileal digestible (SID) Lys levels of the diet on the growth performance, carcass characteristics, and meat quality of entire males (EM), surgical castrates (SC), and males vaccinated with a gonadotropin-releasing hormone (GnRH) vaccine (GV). In total, 252 crossbred pigs were fed 6 different experimental diets consisting of 3 sexes (EM, SC, and GV pigs) and 2 dietary SID Lys levels (2008 CVB standard SID Lys recommendation [Std] or 15% extra SID Lys [+15]) according to a 3-phase feeding scheme (d 0 to 35, 35 to 70, and 70 to 105 of experiment) with 6 pens per treatment and 6 pigs per pen. To determine if dietary SID Lys could be reduced for GV males after the second vaccination, an additional dietary treatment was included (GV+15-Std). The GV males on the GV+15-Std were fed the diet with 15% extra SID Lys in the starter and grower phases and the standard SID Lys level in the finisher phase. First vaccination was administered to pigs at the start of the experiment (23.4 kg BW and 63.6 d of age), and the second vaccination occurred 6 wk before slaughter (77.2 kg BW and 126.6 d of age). Until the second vaccination, GV pigs showed a lower (P ≤ 0.05) ADFI than SC but similar to EM. After the second vaccination, GV pigs had an intermediate (P ≤ 0.05) G:F between EM and SC. The GV+15 pigs showed a better G:F (P ≤ 0.05) than GV pigs fed the GV+15-Std diet in the finisher phase. No differences in the growth rate of the pigs were observed. The SC had a greater (P ≤ 0.05) carcass weight and dressing percentage than EM and GV pigs. The GV-Std pigs had a lower (P ≤ 0.05) meat percentage and greater (P ≤ 0.05) backfat thickness than the GV+15 pigs. The GV pigs and SC fed the standard SID Lys diet had a greater (P ≤ 0.05) percentage of SFA in the subcutaneous fat than EM+15 pigs. On the other hand, SC showed the greatest (P ≤ 0.05) concentration of MUFA compared to EM and GV pigs. The SC and GV pigs

  1. St. Paul's Pig Pack.

    ERIC Educational Resources Information Center

    Miller, Penny Folley

    1982-01-01

    Describes a guinea pig (cavy) breeding and management program developed as part of an elementary school science curriculum. Includes comments on show competitions (sponsored by the American Rabbit Breeders Association) to measure the success of the breeding program and to enable children to experience the business world. (Author/JN)

  2. A Simple "Pig" Game

    ERIC Educational Resources Information Center

    Johnson, Roger W.

    2008-01-01

    Our pig game involves a series of tosses of a die with the possibility of a player's score improving with each additional toss. With each additional toss, however, there is also the chance of losing the entire score accumulated so far. Two different strategies for deciding how many tosses a player should attempt are developed and then compared in…

  3. Feeding Jerusalem artichoke reduced skatole level and changed intestinal microbiota in the gut of entire male pigs.

    PubMed

    Vhile, S G; Kjos, N P; Sørum, H; Overland, M

    2012-05-01

    Different levels of dried Jerusalem artichoke were fed to entire male pigs 1 week before slaughter. The objective was to investigate the effect on skatole level in the hindgut and in adipose tissue, as well as the effect on microflora and short-chain fatty acids (SCFA) in the hindgut. Five experimental groups (n = 11) were given different dietary treatments 7 days before slaughtering: negative control (basal diet), positive control (basal diet + 9% chicory-inulin), basal diet + 4.1% Jerusalem artichoke, basal diet + 8.1% Jerusalem artichoke and basal diet + 12.2% Jerusalem artichoke. Samples from colon, rectum, faeces and adipose tissue were collected. Effect of dietary treatment on skatole, indole and androstenone levels in adipose tissue and on skatole, indole, pH, dry matter (DM), microbiota and SCFA in the hindgut was evaluated. Feeding increasing levels of Jerusalem artichoke to entire male pigs reduced skatole in digesta from colon and in faeces (linear, P < 0.01). There was also a tendency towards a decreased level of skatole in adipose tissue (linear, P = 0.06). Feeding Jerusalem artichoke decreased DM content in colon and faeces and pH in colon (linear, P < 0.01). Increasing levels of Jerusalem artichoke resulted in a reduced level of Clostridium perfringens in both colon and rectum (linear, P < 0.05) and a tendency towards decreased levels of enterobacteria in colon (linear, P = 0.05). Further, there was an increase in total amount of SCFA (linear, P < 0.05), acetic acid (linear, P < 0.05) and valerianic acid (linear, P < 0.01) in faeces. In conclusion, adding dried Jerusalem artichoke to diets for entire male pigs 1 week before slaughter resulted in a dose-dependent decrease in skatole levels in the hindgut and adipose tissue. The reduced skatole levels might be related to the decrease in C. perfringens and the increase in SCFA with subsequent reduction in pH.

  4. Estimation of calcium and phosphorus content in growing and finishing pigs: whole empty body components and relative accretion rates.

    PubMed

    Pettey, L A; Cromwell, G L; Jang, Y D; Lindemann, M D

    2015-01-01

    Two comparative serial-slaughter experiments were conducted to determine whole empty body (WEB) composition and accretion rates of Ca and P in 18 to 109 kg BW pigs to provide information for modeling of these nutrients for growth. Both studies were conducted with 5 sets of 5 littermate barrows which were allotted to 5 slaughter groups in each study (Exp. 1: 18, 27, 36, 45, and 54 kg BW; Exp. 2: 36, 54, 73, 91, and 109 kg BW). Pigs were fed corn-soybean meal-based diets fortified with minerals and vitamins in 2 dietary phases in Exp. 1 (Phase 1: 18 to 36 kg BW; Phase 2: 36 to 54 kg BW) and 3 dietary phases in Exp. 2 (Phase 2: 36 to 54 kg BW; Phase 3: 54 to 78 kg BW; and Phase 4: 78 to 109 kg BW). At the predetermined BW, pigs were slaughtered and separated into body components of hair, hooves, blood, head, viscera, and carcass. The carcass was split along the dorsal midline and the left carcass side was ground for chemical analysis. Whole empty body weight averaged 93.6% and 94.0% of live BW in Exp. 1 and Exp. 2, respectively. As WEB weight increased in both experiments, the percentage carcass of the WEB linearly (P < 0.05) increased, the percentage viscera linearly (P < 0.05) decreased, and the mass (g) of N, ash, Ca, and P in the WEB increased linearly (R(2) = 0.98). The concentration (g/kg) of P in the WEB of 18 to 54 kg pigs increased from 4.30 to 4.57 (linear; P < 0.05) and for Ca increased from 5.13 to 5.66 (linear; P < 0.05). In Exp. 2, P concentration was not related to WEB weight and Ca concentration increased quadratically (P < 0.05). The relative accretion rate of N to P was 1.00 (R(2) = 0.99) in the pigs from 18 to 54 kg. In conclusion, these results indicate that compositional changes as BW increases are strongly related to P retention and that the quantification of WEB P and relationships of WEB P to other chemical components in the body may be useful for modeling purposes in growing and finishing pigs.

  5. Identification of major Streptococcus suis serotypes 2, 7, 8 and 9 isolated from pigs and humans in upper northeastern Thailand.

    PubMed

    Nutravong, Thitima; Angkititrakul, Sunpetch; Jiwakanon, Netchanok; Wongchanthong, Wanlaya; Dejsirilerts, Surang; Nawa, Yukifumi

    2014-09-01

    Streptococcus suis serotype 2 infections occur in many provinces of north-eastern Thailand, knowledge concerning the prevalence of the common S. suis serotypes (1, 1/2, 2, 5, 7, 8, 9, 14 and 16) among healthy and diseased pigs in upper northeastern Thailand remains limited. This study investigated S. suis isolates from pigs (healthy and diseased) and also from humans using 11 conventional biochemical tests, 16S rDNA PCR and sequence analysis and multiplex PCR genotyping of porcine cps and gdh. Thirty-three isolates were obtained between 2009 and 2012 from blood or cerebrospinal fluid of patients from northeastern Thailand previously diagnosed with S. suis infection, based on clinical symptoms and laboratory diagnosis using 11 biochemical tests and PCR detection of 16S rDNA and cps. Eleven S. suis isolates were obtained between 2006 and 2009 from diseased pigs with clinical signs and laboratory diagnoses. In addition, 43 isolates obtained from 741 nasal swab cultures of slaughtered pigs between 2011 and 2012 were included. All three methods showed similar sensitivity in detection of S. suis from clinical and diseased pig specimens, although in healthy pigs, the 11 conventional biochemical methods yielded 2.3% false positives, and the gdh PCR detection method exhibited 31% false negatives. S. suis was present among healthy pigs in 8 of 10 provinces in upper northeastern Thailand, giving an average prevalence of 5.7% (range 1%-17%) using conventional methods together with 16S rDNA PCR assay. False positives by conventional methods were due to species with similar phenotypes, such as viridian streptococci, and are not statistically different from those obtained with the 16S rDNA PCR method, and the false negatives using gdh PCR assay will require further investigation. As S. suis was recovered from both diseased and healthy pigs, raw or undercooked pork products should be considered unsafe for handling or consumption in these regions of Thailand.

  6. The Danish Cardiac Rehabilitation Database

    PubMed Central

    Zwisler, Ann-Dorthe; Rossau, Henriette Knold; Nakano, Anne; Foghmar, Sussie; Eichhorst, Regina; Prescott, Eva; Cerqueira, Charlotte; Soja, Anne Merete Boas; Gislason, Gunnar H; Larsen, Mogens Lytken; Andersen, Ulla Overgaard; Gustafsson, Ida; Thomsen, Kristian K; Boye Hansen, Lene; Hammer, Signe; Viggers, Lone; Christensen, Bo; Kvist, Birgitte; Lindström Egholm, Cecilie; May, Ole

    2016-01-01

    Aim of database The Danish Cardiac Rehabilitation Database (DHRD) aims to improve the quality of cardiac rehabilitation (CR) to the benefit of patients with coronary heart disease (CHD). Study population Hospitalized patients with CHD with stenosis on coronary angiography treated with percutaneous coronary intervention, coronary artery bypass grafting, or medication alone. Reporting is mandatory for all hospitals in Denmark delivering CR. The database was initially implemented in 2013 and was fully running from August 14, 2015, thus comprising data at a patient level from the latter date onward. Main variables Patient-level data are registered by clinicians at the time of entry to CR directly into an online system with simultaneous linkage to other central patient registers. Follow-up data are entered after 6 months. The main variables collected are related to key outcome and performance indicators of CR: referral and adherence, lifestyle, patient-related outcome measures, risk factor control, and medication. Program-level online data are collected every third year. Descriptive data Based on administrative data, approximately 14,000 patients with CHD are hospitalized at 35 hospitals annually, with 75% receiving one or more outpatient rehabilitation services by 2015. The database has not yet been running for a full year, which explains the use of approximations. Conclusion The DHRD is an online, national quality improvement database on CR, aimed at patients with CHD. Mandatory registration of data at both patient level as well as program level is done on the database. DHRD aims to systematically monitor the quality of CR over time, in order to improve the quality of CR throughout Denmark to benefit patients. PMID:27822083

  7. The Danish Communicative Developmental Inventories: Validity and Main Developmental Trends

    ERIC Educational Resources Information Center

    Bleses, Dorthe; Vach, Werner; Slott, Malene; Wehberg, Sonja; Thomsen, Pia; Madsen, Thomas O.; Basboll, Hans

    2008-01-01

    This paper presents a large-scale cross-sectional study of Danish children's early language acquisition based on the Danish adaptation of the "MacArthur-Bates Communicative Development Inventories" (CDI). Measures of validity and reliability imply that the Danish adaptation of the American CDI has been adjusted linguistically and culturally in…

  8. 9 CFR 73.2 - Interstate shipment for immediate slaughter from quarantined or nonquarantined areas; conditions...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Interstate shipment for immediate slaughter from quarantined or nonquarantined areas; conditions under which permitted. 73.2 Section 73.2 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF...

  9. 9 CFR 73.3 - Shipment for purposes other than slaughter; conditions under which permitted.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Shipment for purposes other than slaughter; conditions under which permitted. 73.3 Section 73.3 Animals and Animal Products ANIMAL AND PLANT... POULTRY) AND ANIMAL PRODUCTS SCABIES IN CATTLE § 73.3 Shipment for purposes other than...

  10. 9 CFR 73.3 - Shipment for purposes other than slaughter; conditions under which permitted.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Shipment for purposes other than slaughter; conditions under which permitted. 73.3 Section 73.3 Animals and Animal Products ANIMAL AND PLANT... POULTRY) AND ANIMAL PRODUCTS SCABIES IN CATTLE § 73.3 Shipment for purposes other than...

  11. 9 CFR 73.2 - Interstate shipment for immediate slaughter from quarantined or nonquarantined areas; conditions...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Interstate shipment for immediate slaughter from quarantined or nonquarantined areas; conditions under which permitted. 73.2 Section 73.2 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF...

  12. 9 CFR 93.518 - Swine from Canada for immediate slaughter.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Swine from Canada for immediate slaughter. 93.518 Section 93.518 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE EXPORTATION AND IMPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL...

  13. 9 CFR 73.2 - Interstate shipment for immediate slaughter from quarantined or nonquarantined areas; conditions...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Interstate shipment for immediate slaughter from quarantined or nonquarantined areas; conditions under which permitted. 73.2 Section 73.2 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF...

  14. 9 CFR 73.3 - Shipment for purposes other than slaughter; conditions under which permitted.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Shipment for purposes other than slaughter; conditions under which permitted. 73.3 Section 73.3 Animals and Animal Products ANIMAL AND PLANT... POULTRY) AND ANIMAL PRODUCTS SCABIES IN CATTLE § 73.3 Shipment for purposes other than...

  15. Halal stunning and slaughter: Criteria for the assessment of dead animals.

    PubMed

    Fuseini, Awal; Knowles, Toby G; Hadley, Phil J; Wotton, Steve B

    2016-09-01

    The debate surrounding the acceptability of stunning for Halal slaughter is one that is likely to linger. Compared to a couple of decades or so ago, one may argue that pre-slaughter stunning is becoming a popular practice during Halal slaughter due to the increasing number of Muslim-majority countries who continue to issue religious rulings (Fatwa) to approve the practice. Concerns have often, however been raised about the likelihood of some animals dying as a result of stunning and whether there are mechanisms in place to identify and remove dead animals stunned with irreversible techniques before their necks are cut. This paper reviews literature about what makes meat Halal, considers the arguments put forward by proponents and opponents of pre-slaughter stunning for Halal production and examines the criteria used by Halal Certification Bodies to identify and reject animals that may die as a result of irreversible stunning and considers the specific risks of waterbath stunning (for poultry) from a Halal viewpoint.

  16. 9 CFR 93.518 - Swine from Canada for immediate slaughter.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Swine from Canada for immediate slaughter. 93.518 Section 93.518 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE EXPORTATION AND IMPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL...

  17. 9 CFR 93.518 - Swine from Canada for immediate slaughter.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Swine from Canada for immediate slaughter. 93.518 Section 93.518 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE EXPORTATION AND IMPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL...

  18. 9 CFR 73.3 - Shipment for purposes other than slaughter; conditions under which permitted.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... POULTRY) AND ANIMAL PRODUCTS SCABIES IN CATTLE § 73.3 Shipment for purposes other than slaughter; conditions under which permitted. Cattle affected with scabies may be shipped interstate for any purpose if... State inspector, and so certified by such inspector, or such cattle may be so shipped if dipped once...

  19. 9 CFR 73.3 - Shipment for purposes other than slaughter; conditions under which permitted.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Shipment for purposes other than slaughter; conditions under which permitted. 73.3 Section 73.3 Animals and Animal Products ANIMAL AND PLANT... POULTRY) AND ANIMAL PRODUCTS SCABIES IN CATTLE § 73.3 Shipment for purposes other than...

  20. John Slaughter Retires from Occidental College Having Achieved Ambitious Diversity Goals.

    ERIC Educational Resources Information Center

    Basinger, Julianne

    1999-01-01

    President John Brooks Slaughter has pushed to create diversity at all levels at Occidental College (California), including students, faculty, staff, administrators, and trustees, while maintaining academic excellence. Many say he has succeeded in his leadership, although some alumni are critical of the changes and some supporters are concerned…

  1. 9 CFR 381.190 - Transactions in slaughtered poultry and other poultry products restricted; vehicle sanitation...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 2 2014-01-01 2014-01-01 false Transactions in slaughtered poultry and other poultry products restricted; vehicle sanitation requirements. 381.190 Section 381.190... ORGANIZATION AND TERMINOLOGY; MANDATORY MEAT AND POULTRY PRODUCTS INSPECTION AND VOLUNTARY INSPECTION...

  2. 9 CFR 381.190 - Transactions in slaughtered poultry and other poultry products restricted; vehicle sanitation...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 2 2011-01-01 2011-01-01 false Transactions in slaughtered poultry and other poultry products restricted; vehicle sanitation requirements. 381.190 Section 381.190... ORGANIZATION AND TERMINOLOGY; MANDATORY MEAT AND POULTRY PRODUCTS INSPECTION AND VOLUNTARY INSPECTION...

  3. 9 CFR 381.190 - Transactions in slaughtered poultry and other poultry products restricted; vehicle sanitation...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Transactions in slaughtered poultry and other poultry products restricted; vehicle sanitation requirements. 381.190 Section 381.190... ORGANIZATION AND TERMINOLOGY; MANDATORY MEAT AND POULTRY PRODUCTS INSPECTION AND VOLUNTARY INSPECTION...

  4. 9 CFR 381.190 - Transactions in slaughtered poultry and other poultry products restricted; vehicle sanitation...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 2 2012-01-01 2012-01-01 false Transactions in slaughtered poultry and other poultry products restricted; vehicle sanitation requirements. 381.190 Section 381.190... ORGANIZATION AND TERMINOLOGY; MANDATORY MEAT AND POULTRY PRODUCTS INSPECTION AND VOLUNTARY INSPECTION...

  5. 9 CFR 381.190 - Transactions in slaughtered poultry and other poultry products restricted; vehicle sanitation...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 2 2013-01-01 2013-01-01 false Transactions in slaughtered poultry and other poultry products restricted; vehicle sanitation requirements. 381.190 Section 381.190... ORGANIZATION AND TERMINOLOGY; MANDATORY MEAT AND POULTRY PRODUCTS INSPECTION AND VOLUNTARY INSPECTION...

  6. Breed, slaughter weight and ageing time effects on physico-chemical characteristics of lamb meat.

    PubMed

    Martínez-Cerezo, S; Sañudo, C; Panea, B; Medel, I; Delfa, R; Sierra, I; Beltrán, J A; Cepero, R; Olleta, J L

    2005-02-01

    The effects of breed, slaughter weight and ageing time on the meat quality of the three most important Spanish breeds were considered. Two hundred and twenty-five lambs of Rasa Aragonesa-local meat breed-, Churra-local dairy breed- and Spanish Merino were used. Animals (75 of each breed) were slaughtered at three different live weights (10-12, 20-22 or 30-32 kg), and the meat was aged for 1, 2, 4, 8 or 16 days. The meat pH, colour, amount of haem pigments, intramuscular fat, moisture, hydroxyproline content and sarcomere length were measured at 24 h post-mortem. Meat texture was measured by compression after each ageing time. The pH of the samples ranged from 5.50 to 5.58. Meat colour varied with breed and slaughter weight (P⩽0.01), the M. longissimus thoracis was lighter in the youngest animals and in the Churra breed and redder in Merinos. Intramuscular fat increased and moisture decreased for heavier lambs. Differences in collagen were associated with breed (P⩽0.01); total and insoluble collagen contents were higher in the Churra breed. Sarcomere length was only slightly affected by slaughter weight. Meat from the Churra breed had the highest values at high levels of compression. Suckling lambs (10-12 kg) had greater myofibrillar toughness than heavier lambs and ageing strongly influenced myofibrillar tenderness.

  7. Meat quality of lamb: Pre-slaughter fattening on cultivated or mountain range pastures.

    PubMed

    Lind, Vibeke; Berg, Jan; Eik, Lars Olav; Mølmann, Jørgen; Haugland, Espen; Jørgensen, Marit; Hersleth, Margrethe

    2009-12-01

    Many consumers perceive lamb meat from mountain pastures to be of superior quality, a quality that may be altered if lambs are kept for a longer period on cultivated pastures before slaughtering. The objective of this experiment was to compare sensory profile and fatty acid composition in meat from lambs slaughtered directly from unimproved mountain pastures with meat from lambs raised on unimproved mountain pastures and fattened on biodiverse cultivated pastures for 26, 39 and 42days before slaughtering. The experiment was conducted at two different locations in Norway in 2006 and 2007, with a total of 124 Norwegian Crossbred Sheep lambs. Loin samples of M. Longissimus dorsi from lambs above a body weight of 40kg were selected and analysed for sensory attributes. Fatty acid composition was determined in the subcutaneous fat over the Longissimus dorsi. Small but significant differences were found in hardness, tenderness, fattiness, metallic and rancid flavour, and in polyunsaturated fatty acids. This indicates that to a small extent pre-slaughter fattening on cultivated pastures alters meat characteristics.

  8. Slaughtering for a living: A hermeneutic phenomenological perspective on the well-being of slaughterhouse employees

    PubMed Central

    Victor, Karen; Barnard, Antoni

    2016-01-01

    Slaughterhouses constitute a unique work setting exposing employees to particular physical and psychological health challenges. Research that focuses on the well-being of slaughterhouse employees is limited, and the aim of this study was to explore their well-being by conducting a hermeneutic phenomenological study of specifically the slaughterfloor employees’ work-life experiences. The study was conducted in a South African commercial abattoir setting. Thirteen slaughterfloor employees and two managers of the slaughterfloor section participated in unstructured interviews. A hermeneutic phenomenological approach to data analysis was adopted following the stages of a naïve reading, a structural thematic analysis, and a comprehensive understanding. Data analysis resulted in four process-related themes representing the different stages of becoming a slaughterer, (mal)adjusting to slaughter work, coping with and maintaining the work, and living with the psycho-social consequences of slaughter work. Results facilitate an understanding of how employee well-being manifests in each of these stages of being a slaughterfloor employee. The risk potential of employees suffering from post-traumatic stress syndrome was evident throughout the stages of being a slaughterfloor employee and offers a useful diagnostic framework to facilitate employee well-being assistance. Slaughterhouse management should develop a holistic focus addressing employee well-being needs evident in each of the stages of being a slaughter worker and by extending well-being interventions to the broader communities that the slaughterhouse functions in. PMID:27104340

  9. Mineral retention of serially slaughtered Holstein steers supplemented with zilpaterol hydrochloride

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Holstein steers (n = 115; 449 ± 20 kg) were utilized in a serial harvest trial measuring Ca, P, Mg, K, and S retention. A baseline group of 5 steers was harvested after 226 days on feed, which was designated d 0. Remaining cattle were assigned to 11 harvest groups, with slaughter every 28 d. Prio...

  10. Quantitative analysis of production traits in saltwater crocodiles (Crocodylus porosus): II. age at slaughter.

    PubMed

    Isberg, S R; Thomson, P C; Nicholas, F W; Barker, S G; Moran, C

    2005-12-01

    Crocodile morphometric (head, snout-vent and total length) measurements were recorded at three stages during the production chain: hatching, inventory [average age (+/-SE) is 265.1 +/- 0.4 days] and slaughter (average age is 1037.8 +/- 0.4 days). Crocodile skins are used for the manufacture of exclusive leather products, with the most common-sized skin sold having 35-45 cm in belly width. One of the breeding objectives for inclusion into a multitrait genetic improvement programme for saltwater crocodiles is the time taken for a juvenile to reach this size or age at slaughter. A multivariate restricted maximum likelihood analysis provided (co)variance components for estimating the first published genetic parameter estimates for these traits. Heritability (+/-SE) estimates for the traits hatchling snout-vent length, inventory head length and age at slaughter were 0.60 (0.15), 0.59 (0.12) and 0.40 (0.10) respectively. There were strong negative genetic (-0.81 +/- 0.08) and phenotypic (-0.82 +/- 0.02) correlations between age at slaughter and inventory head length.

  11. Effect of feeding reduced crude protein and phosphorus diets on weaning-finishing pig growth performance, carcass characteristics, and bone characteristics.

    PubMed

    Hinson, R B; Schinckel, A P; Radcliffe, J S; Allee, G L; Sutton, A L; Richert, B T

    2009-04-01

    Two experiments were conducted to determine the effects of feeding a corn- and soybean meal-based diet (control) or a low nutrient excretion (LNE) diet, formulated with reduced dietary CP and additional synthetic AA, low phytic acid corn, and phytase, on pig growth performance during the grower (BW=32 to 77 kg) and finisher (BW=78 to 126 kg) periods and on carcass and bone characteristics at slaughter. Pigs (32+/-1.3 kg of BW, Exp. 1; 6.7+/-0.27 kg of BW, Exp. 2) were blocked by sex and BW and randomly allotted to a control or LNE diet. Pigs were housed in 4 rooms during the nursery period and in 2 rooms during the grower and finisher periods, with individual and identical ventilation systems. Pigs were phase fed 3 nursery diets for 5 wk (Exp. 2) and phase fed 2 grower and 2 finisher diets for 16 wk (Exp. 1 and 2). Pigs were housed 4 or 5 pigs/pen with 9 pens x sex(-1) x treatment(-1) during the nursery period and 4 or 5 pigs/pen with 5 pens x sex(-1) x treatment(-1) in the grower phase and 2 or 3 pigs/pen in the finisher phase. Individual BW and pen feed disappearance were recorded weekly in the nursery period and every 2 wk in the grower-finisher period. Pigs were scanned ultrasonically at d 34 of the nursery period and wk 8 and 16 of the grower-finisher period to determine backfat depths and LM area. Ten pigs x sex(-1) x treatment(-1) were slaughtered at wk 16 of each experiment to determine carcass characteristics. Overall growth performance was not different during each experiment. However, nursery G:F (control=0.65; LNE=0.60), grower ADG (Exp. 1 and 2), and grower G:F (Exp. 2) were reduced (P<0.05) when the LNE diets were fed. Diet had no effect on 10th-rib carcass data in either experiment. Metatarsal bone ash percentage was reduced (P<0.05) when the LNE diets were consumed in both experiments. Feeding LNE diets resulted in the maintenance of overall growth performance, bone variables, and carcass characteristics. However, further refinements are still

  12. Parasite population dynamics in pigs infected with Trichuris suis and Oesophagostomum dentatum.

    PubMed

    Petersen, Heidi Huus; Andreasen, Annette; Kringel, Helene; Roepstorff, Allan; Thamsborg, Stig M

    2014-01-17

    The aim of the present study was to investigate the population dynamics and potential interactions between Trichuris suis and Oesophagostomum dentatum in experimentally co-infected pigs, by quantification of parasite parameters such as egg excretion, worm recovery and worm location. Forty-eight helminth naïve pigs were allocated into four groups. Group O was inoculated with 20 O. dentatum L3/kg/day and Group T with 10 T. suis eggs/kg/day. Group OT was inoculated with both 20 O. dentatum L3/kg/day and 10 T. suis eggs/kg/day, while Group C was kept as an uninfected control group. All inoculations were trickle infections administered twice weekly and were continued until slaughter. Faecal samples were collected from the rectum of all pigs at day 0, and twice weekly from 2 to 9 weeks post first infection (wpi). Six pigs from each group were necropsied 5 wpi and the remaining 6 pigs from each group were necropsied 10 wpi. The faecal egg counts (FEC) and total worm burdens of O. dentatum were dramatically influenced by the presence of T. suis, with significantly lower mean FECs and worm burdens at 5 and 10 wpi compared to single infected pigs. Furthermore, in the presence of T. suis we found that O. dentatum was located more posteriorly in the gut. The changes in the Trichuris population were less prominent, but faecal egg counts, worm counts 5 wpi (57% recovered vs. 39%) and the proportion of infected animals at 10 wpi were higher in Group OT compared to Group T. The location of T. suis was unaffected by the presence of O. dentatum. These results indicate an antagonistic interaction between T. suis and O. dentatum which is dominated by T. suis.

  13. A Quantitative Microbiological Risk Assessment for Salmonella in Pigs for the European Union.

    PubMed

    Snary, Emma L; Swart, Arno N; Simons, Robin R L; Domingues, Ana Rita Calado; Vigre, Hakan; Evers, Eric G; Hald, Tine; Hill, Andrew A

    2016-03-01

    A farm-to-consumption quantitative microbiological risk assessment (QMRA) for Salmonella in pigs in the European Union has been developed for the European Food Safety Authority. The primary aim of the QMRA was to assess the impact of hypothetical reductions of slaughter-pig prevalence and the impact of control measures on the risk of human Salmonella infection. A key consideration during the QMRA development was the characterization of variability between E.U. Member States (MSs), and therefore a generic MS model was developed that accounts for differences in pig production, slaughterhouse practices, and consumption patterns. To demonstrate the parameterization of the model, four case study MSs were selected that illustrate the variability in production of pork meat and products across MSs. For the case study MSs the average probability of illness was estimated to be between 1 in 100,000 and 1 in 10 million servings given consumption of one of the three product types considered (pork cuts, minced meat, and fermented ready-to-eat sausages). Further analyses of the farm-to-consumption QMRA suggest that the vast majority of human risk derives from infected pigs with a high concentration of Salmonella in their feces (≥10(4) CFU/g). Therefore, it is concluded that interventions should be focused on either decreasing the level of Salmonella in the feces of infected pigs, the introduction of a control step at the abattoir to reduce the transfer of feces to the exterior of the pig, or a control step to reduce the level of Salmonella on the carcass post-evisceration.

  14. Serological examination of fattening pigs reveals associations between Ascaris suum, lung pathogens and technical performance parameters.

    PubMed

    Vlaminck, Johnny; Düsseldorf, Simon; Heres, Lourens; Geldhof, Peter

    2015-06-15

    Diagnosing the presence of the highly prevalent and economically important pig parasite Ascaris suum on fattening farms has so far been challenging. Currently, only the number of livers affected at slaughter is routinely used to measure parasite exposure. However, recently, a new serological test was developed based on the detection of antibodies to the A. suum haemoglobin molecule. The test showed to be highly sensitive for the detection of exposure to A. suum in fattening pigs. In this study we first compared the performance of A. suum serology versus the percentage of affected livers at slaughter, subsequently we investigated potential associations between A. suum infection levels and exposure to important lung pathogens and finally we identified correlations between serological data and technical performance parameters (TPIs) from 20 Belgian and 20 German pig fattening farms. In both Belgian and German farms, a significant relationship was detected between elevated average Ascaris serology and percentages of affected livers (ρ=0.63 and ρ=0.75, respectively). On the Belgian farms, both Ascaris serology and the percentage of affected livers were negatively correlated with average daily gain (ADG) (ρ=-0.69 and ρ=-0.56, respectively). Using the German dataset, only a borderline negative association was detected between the percentage of affected livers and the ADG (ρ=-0.44, P=0.053). In contrast, only in the German farms, correlations between the percentage of affected lungs at slaughter and elevated presence of A. suum and several other airway pathogens were detected. To conclude, this study indicates that serological screening for A. suum on fattening farms is an attractive new diagnostic tool that can be used to indicate the presence of roundworm infection by measuring infection intensity. Furthermore the results of this study also add weight to the evidence that both roundworm infections as well as herd exposure to airway pathogens have a significant

  15. Integrating economic parameters into genetic selection for Large White pigs.

    PubMed

    Dube, Bekezela; Mulugeta, Sendros D; Dzama, Kennedy

    2013-08-01

    The objective of the study was to integrate economic parameters into genetic selection for sow productivity, growth performance and carcass characteristics in South African Large White pigs. Simulation models for sow productivity and terminal production systems were performed based on a hypothetical 100-sow herd, to derive economic values for the economically relevant traits. The traits included in the study were number born alive (NBA), 21-day litter size (D21LS), 21-day litter weight (D21LWT), average daily gain (ADG), feed conversion ratio (FCR), age at slaughter (AGES), dressing percentage (DRESS), lean content (LEAN) and backfat thickness (BFAT). Growth of a pig was described by the Gompertz growth function, while feed intake was derived from the nutrient requirements of pigs at the respective ages. Partial budgeting and partial differentiation of the profit function were used to derive economic values, which were defined as the change in profit per unit genetic change in a given trait. The respective economic values (ZAR) were: 61.26, 38.02, 210.15, 33.34, -21.81, -68.18, 5.78, 4.69 and -1.48. These economic values indicated the direction and emphases of selection, and were sensitive to changes in feed prices and marketing prices for carcasses and maiden gilts. Economic values for NBA, D21LS, DRESS and LEAN decreased with increasing feed prices, suggesting a point where genetic improvement would be a loss, if feed prices continued to increase. The economic values for DRESS and LEAN increased as the marketing prices for carcasses increased, while the economic value for BFAT was not sensitive to changes in all prices. Reductions in economic values can be counterbalanced by simultaneous increases in marketing prices of carcasses and maiden gilts. Economic values facilitate genetic improvement by translating it to proportionate profitability. Breeders should, however, continually recalculate economic values to place the most appropriate emphases on the respective

  16. 9 CFR 203.16 - Mailing of checks in payment for livestock purchased for slaughter, for cash and not on credit.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... livestock purchased for slaughter, for cash and not on credit. 203.16 Section 203.16 Animals and Animal... of checks in payment for livestock purchased for slaughter, for cash and not on credit. (a) The Grain... sells livestock to a packer, market agency, or dealer, who is purchasing for slaughter, may not...

  17. Impact of the separate pre-slaughter stages on broiler chicken welfare.

    PubMed

    Jacobs, Leonie; Delezie, Evelyne; Duchateau, Luc; Goethals, Klara; Tuyttens, Frank A M

    2017-02-01

    Before broilers are slaughtered, several parties are involved in catching, loading, transportation and lairage. During these pre-slaughter activities, broilers are exposed to a variety of stressors. A detailed understanding of how broiler welfare is impaired during each stage could help the responsible parties to make targeted improvements. The aim of this study was to identify welfare problems occurring during the consecutive stages of commercial broiler transportation and to identify risk factors associated with the identified welfare problems. Commercial Belgian transports (n = 81) were assessed in spring (n = 14), summer (n = 33), autumn (n = 10), and winter (n = 24), and potential risk factors were recorded by the observer. Animal-based welfare indicators were scored before the start of the pre-slaughter phase as well as after the catching, transport and lairage, and slaughter stages to assess the impact of each stage. The most frequently observed welfare impairments were vent and thigh lesions, panting, wing fractures, and bruising on wings and breasts. Our results show that the impact of the pre-slaughter phase on broiler welfare is multifaceted. The overall pre-slaughter phase resulted in a mean weight decrease of 5.3%, a prevalence of 1.4% in leg bruising, and 3.7% in breast or wing bruising. Wing fractures occurred mainly during the catching stage: Prevalence increased from 0.1% to 1.9% (P = 0.003). A welfare comparison before and after transportation and lairage revealed that plumage had become more soiled (P = 0.003), body temperature decreased by 0.7°C (P < 0.001), huddling prevalence increased by 0.5% (P = 0.008), prevalence of birds with splayed legs increased by 0.08% (P = 0.008), prevalence of supine birds decreased by 0.05% (P = 0.003), and 0.1% fewer birds with wings stuck in the crates (P = 0.010) were observed. Risk factor analyses revealed that carefully choosing the catching crew, minimizing thermal stress, reducing duration of

  18. Effect of slaughter age on foal carcass traits and meat quality.

    PubMed

    Domínguez, R; Crecente, S; Borrajo, P; Agregán, R; Lorenzo, J M

    2015-10-01

    Meat has played a crucial role in human evolution and is an important component of a healthy and well-balanced diet due to its nutritional richness. Recent studies have shown that horsemeat may be considered as an alternative to other meat (such as beef or pork), and it may have a positive effect on human health from a nutritional point of view. This research was conducted to characterize the carcass measurement, meat quality (chemical composition, colour characteristics and textural traits) and nutritional value (fatty acid and amino acid composition) of foals slaughtered at 8 and 11 months of age (8 and 11 m groups). For this study, a total of 21 foals (10 and 11 animals from the 8 and 11-m groups, respectively) were used. The results obtained showed a positive influence on carcass characteristics with an increase in slaughter age, because 11 m animals had slightly higher values of live (275 v. 247 kg) and carcass weights (148 v. 133 kg), length of leg (72.86 v. 69.85 cm) and carcass (100.41 v. 96.30 cm) and perimeter of leg (97.68 v. 89.22 cm) compared with animals from the 8-m group. Regarding meat quality, only Fe-haeme and cholesterol content in chemical composition and luminosity (L*) in colour parameters showed significant differences. Foals from the 8-m group had the highest content of cholesterol (0.47 v. 0.28 mg/100 g of meat) and luminosity values (39.66 v. 37.88) and the lowest content of ash (1.20% v. 1.40%). In fatty acids content, only five out of 23 fatty acids showed differences between the two groups. However, an interesting change in the fatty acid profile occurred with an increase in the slaughter age. Foals from the 8-m group had the highest values of α-linolenic acid and n-3 fatty acids and the lowest values of linoleic and n-6 fatty acids, which is an interesting fact from a health point of view. Finally, slaughter age had no statistical influence on textural properties or amino acid content. As a main conclusion, animals slaughtered at 8

  19. A Genetic Analysis of Mortality in Pigs

    PubMed Central

    Varona, Luis; Sorensen, Daniel

    2010-01-01

    An analysis of mortality is undertaken in two breeds of pigs: Danish Landrace and Yorkshire. Zero-inflated and standard versions of hierarchical Poisson, binomial, and negative binomial Bayesian models were fitted using Markov chain Monte Carlo (MCMC). The objectives of the study were to investigate whether there is support for genetic variation for mortality and to study the quality of fit and predictive properties of the various models. In both breeds, the model that provided the best fit to the data was the standard binomial hierarchical model. The model that performed best in terms of the ability to predict the distribution of stillbirths was the hierarchical zero-inflated negative binomial model. The best fit of the binomial hierarchical model and of the zero-inflated hierarchical negative binomial model was obtained when genetic variation was included as a parameter. For the hierarchical binomial model, the estimate of the posterior mean of the additive genetic variance (posterior standard deviation in brackets) at the level of the logit of the probability of a stillbirth was 0.173(0.039) in Landrace and 0.202(0.048) in Yorkshire. The implications of these results from a breeding perspective are briefly discussed. PMID:19901070

  20. Effects of the Truck Suspension System on Animal Welfare, Carcass and Meat Quality Traits in Pigs

    PubMed Central

    Dalla Costa, Filipe Antônio; Lopes, Letícia S.; Dalla Costa, Osmar Antônio

    2017-01-01

    Simple Summary Transportation is a complex stressor in which animals are exposed to a series negatively stimuli, such as vibration, new environmental conditions, variation in temperature and humidity, social mixing, noises among other poor factors, which can result in welfare problems and economic losses such as increased skin lesions, poorer pork quality traits. Transport stress may be reduced through a vehicle suspension system that provides a much smoother ride during transport, and consequently is less aversive to pigs. However, air suspension systems are more expensive and have bigger maintenance costs. This increase in transportation cost must be supported by the benefits from improvements in quality of freight transport; otherwise, the truckers will be paying unnecessarily for a similar or equivalent ride quality. Thus, finishing pigs were assessed after transport to slaughter by the same two double-decked trucks using two types of commercial vehicle suspension, leaf-spring and air suspension, to compare effects on blood cortisol and lactate at exsanguination, behaviour during lairage, and carcass (skin lesions) and pork quality traits. The use of leaf-spring suspension system negatively affects the welfare of pigs due to the increased carcass damage and resulted in poorer pork quality traits. Abstract The objective of this study was to assess the effects of two types of commercial suspension (leaf-spring (LS) vs. air suspension (AS)) installed on two similar double-decked trucks on blood cortisol and lactate concentration, lairage behavior, carcass skin lesions and pork quality traits of 120 crossbred pigs. The suspension type neither influenced pig behaviour in lairage nor blood cortisol and lactate concentrations (p > 0.10). However, when compared with the AS suspension system, the use of LS increased the number of skin lesions in the back and thigh (p = 0.03 and p = 0.01, respectively) and produced thigh with lower pHu (p < 0.001) and yellower colour

  1. Fatty acid profile of hair lambs and their crossbreds slaughtered at different weights.

    PubMed

    Landim, Aline Vieira; Cardoso, Maximiliano Tadeu Memória; Castanheira, Marlos; Fioravanti, Maria Clorinda Soares; Louvandini, Helder; McManus, Concepta

    2011-12-01

    This study aims to investigate the effect of breed and slaughter weight on fatty the acid profile of the Longissimus dorsi muscle in lambs. Twenty-four Santa Inês (SI), 24.5 Ile de France × 0.5 Santa Inês (Ile × SI) and 12.5 Texel × 0.5 Santa Inês (Te × SI), slaughtered at different weights (30, 35, 40, and 45 kg), were evaluated. The animals were reared with creep feeding to weaning and were feedlot finished, receiving a diet composed of 30% hay and 70% concentrate. The analyses of fatty acids were carried out on the Longissimus muscle of the 13th rib. The experiment was in a three by four factorial design. The total mean saturated fatty acid level was 44.88%, with 43.30% monounsaturated fats and 1.72% polyunsaturated. The major fatty acids found included oleic (43%), palmitic (22%), and stearic (18%). The concentration of desirable fatty acids varied from 61.56% to 66.78%, with Te × SI (66.78%) having the highest levels (P < 0.05). The slaughter weight affected (P < 0.05) the saturated and unsaturated (both mono and poly) fatty acid profiles. The 35-kg slaughter weight showed the most desirable fatty acid profile. The saturated myristic and stearic fatty acids decreased with an increase in the slaughter weight, but oleic acid increased. In the conditions of the present study, the meat from Santa Ines and Texel × Santa Ines lambs had fatty acid profiles more beneficial for human health due to the higher levels of unsaturated fatty acids.

  2. Influence of diet on the experimental infection of pigs with Brachyspira pilosicoli.

    PubMed

    Lindecrona, R H; Jensen, T K; Møller, K

    2004-02-28

    The effects of five different diets on the experimental infection of pigs with a Danish field isolate of Brachyspira pilosicoli were investigated. The diets tested were a pelleted and a non-pelleted standard diet based on wheat and barley, the standard diet supplemented with 2 per cent lactic acid, a fermented liquid feed and a diet based on cooked rice. Two trials were conducted, each with six groups of six pigs; in each, two of the groups were fed the standard diet. One of these groups and the other four groups were challenged after two weeks on the diets and euthanased four weeks later. The clinical signs of B pilosicoli infection varied from loose stools to watery, mucoid diarrhoea. The group fed the rice diet excreted B pilosicoli in their faeces for a significantly shorter period than the group fed the standard diet (P < 0.01), and fewer of them excreted the organism (P < 0.05). All the pigs fed the pelleted diet excreted B pilosicoli in their faeces, and significantly more of them showed clinical signs of disease than the pigs fed the standard diet (P < 0.05). The fermented liquid feed and the diet containing lactic acid had no significant effect on the excretion of B pilosicoli or on the numbers of pigs showing clinical signs of disease.

  3. Nutritive value of three tropical forage legumes and their influence on growth performance, carcass traits and organ weights of pigs.

    PubMed

    Kambashi, Bienvenu; Kalala, Gaetan; Dochain, Denis; Mafwila, Jacques; Rollin, Xavier; Boudry, Christelle; Picron, Pascale; Bindelle, Jérôme

    2016-08-01

    The effects of tropical forage legumes on feed intake, growth performance and carcass traits were investigated in 16 groups of two Large White × Duroc pigs. The diets consisted of a commercial corn-soybean meal diet as the basal diet and three forage-supplemented diets. Four groups of control pigs received daily 4 % of body weight of the basal diet, and 12 groups of experimental pigs were fed the basal diet at 3.2 % of body weight completed with fresh leaves of one of the three forage legumes (Psophocarpus scandens, Stylosanthes guianensis and Vigna unguiculata) ad libitum. The study lasted 90 days. The in vitro digestion and fermentation of the forage legumes were also determined. The in vitro digestible energy content of the legumes was between 0.72 and 0.77 that of the basal diet (14.4 MJ/kg dry matter (DM)). V . unguiculata was the most digestible forage legume expected for crude protein digestibility. Feeding forage legumes lowered the dry matter intake by 4.5 to 9.6 % (P < 0.05), final body weight (P = 0.013), slaughter weight, average daily gain and hot carcass weight (P < 0.05) without affecting the feed conversion ratio (FCR), dressing percentage and back fat thickness. In conclusion, using forage to feed pig could be interesting in pig smallholder production with limited access to concentrate, as FCR was not significantly affected.

  4. Lean meat prediction with HGP, CGM and CSB-Image-Meater, with prediction accuracy evaluated for different proportions of gilts, boars and castrated boars in the pig population.

    PubMed

    Engel, B; Lambooij, E; Buist, W G; Vereijken, P

    2012-02-01

    Prediction equations for the percentage lean meat in pig carcasses in The Netherlands were derived for the Hennessy Grading Probe 7, Capteur Gras/Maigre--Sydel and CSB-Image-Meater. Because castrated males are expected to vanish from the Dutch pig population in the near future, accuracy of prediction was evaluated for different scenarios representing a wide range of different proportions for entire males, castrated males and females in the Dutch pig population. The prediction equations for the instruments are in compliance with the EC regulations for prediction accuracy for the different scenarios. So, these equations will remain valid when castrated males are (gradually) removed from the Dutch slaughter population. Results of this study are of interest for researchers from countries or areas contemplating the use of one of the aforementioned instruments. The statistical approach for evaluation of prediction accuracy is of particular interest when changes in proportions of important subpopulations in the target population are foreseen.

  5. Persistence of a wild type Escherichia coli and its multiple antibiotic-resistant (MAR) derivatives in the abattoir and on chilled pig carcasses.

    PubMed

    Delsol, Anne A; Halfhide, Deborah E; Bagnall, Mary C; Randall, Luke P; Enne, Virve I; Woodward, Martin J; Roe, John M

    2010-06-15

    The aim of this study was to evaluate the ability of an Escherichia coli with the multiple antibiotic resistance (MAR) phenotype to withstand the stresses of slaughter compared to an isogenic progenitor strain. A wild type E. coli isolate (345-2RifC) of porcine origin was used to derive 3 isogenic MAR mutants. Escherichia coli 345-2RifC and its MAR derivatives were inoculated into separate groups of pigs. Once colonisation was established, the pigs were slaughtered and persistence of the E. coli strains in the abattoir environment and on the pig carcasses was monitored and compared. No significant difference (P>0.05) was detected between the shedding of the different E. coli strains from the live pigs. Both the parent strain and its MAR derivatives persisted in the abattoir environment, however the parent strain was recovered from 6 of the 13 locations sampled while the MAR derivatives were recovered from 11 of 13 and the number of MAR E. coli recovered was 10-fold higher than the parent strain at half of the locations. The parent strain was not recovered from any of the 6 chilled carcasses whereas the MAR derivatives were recovered from 3 out of 5 (P<0.001). This study demonstrates that the expression of MAR in 345-2RifC increased its ability to survive the stresses of the slaughter and chilling processes. Therefore in E. coli, MAR can give a selective advantage, compared to non-MAR strains, for persistence on chilled carcasses thereby facilitating transit of these strains through the food chain.

  6. Impact of potential changes to the current bovine spongiform encephalopathy surveillance programs for slaughter cattle and fallen stock in Japan.

    PubMed

    Sugiura, Katsuaki; Murray, Noel; Shinoda, Naoki; Onodera, Takashi

    2009-07-01

    Cattle slaughtered in Japan for human consumption, regardless of their age, have been tested for bovine spongiform encephalopathy (BSE) since October 2001. Beginning in April 2004, all fallen stock from 24 months of age also have been tested. We evaluated the impact of potential changes to the current BSE surveillance programs for both slaughter cattle and fallen stock using a simple stochastic model. We calculated the probability that a BSE-infected dairy cow, Wagyu beef animal, Wagyu-Holstein cross steer or heifer, or Holstein steer slaughtered for human consumption or arising as fallen stock would be tested and detected. Four surveillance strategies were explored for cattle slaughtered for human consumption, with the minimum age at testing set at 0, 21, 31, or 41 months. Three surveillance strategies were explored for fallen stock, with the minimum age at testing set at 24, 31, or 41 months. Increasing the minimum age of testing from 0 to 21 months for both dairy cattle and Wagyu beef cattle had very little impact on the probability that a BSE-infected animal slaughtered for human consumption would be detected. Although increasing the minimum age at testing from 21 to 31 or 41 months would lead to fewer slaughtered animals being tested, the impact on the probability of detecting infected animals would be insignificant. The probability of infected Wagyu-Holstein crosses and Holstein steers being detected at slaughter or as fallen stock would be very low under all surveillance strategies.

  7. Effect of a gonadotropin-releasing factor vaccine on follicle-stimulating hormone and luteinizing hormone concentrations and on the development of testicles and the expression of boar taint in male pigs.

    PubMed

    Fuchs, T; Thun, R; Parvizi, N; Nathues, H; Koehrmann, A; Andrews, S; Brock, F; Klein, G; Sudhaus, N; Grosse Beilage, E

    2009-09-15

    The objective of this study was to determine the effect of using a gonadotropin-releasing factor (GnRF) vaccine on follicle-stimulating hormone (FSH) and luteinizing hormone (LH) concentrations in plasma, the size of testicles, and the expression of boar taint in male pigs. Vaccinated pigs were compared with surgically castrated pigs and entire males. Pigs were randomly assigned to three treatment groups: surgically castrated during the first week of life (T01, n=274), immunized twice during the fattening period with a GnRF vaccine, the first when 13 to 14 wk of age and the second when 20 to 21 wk of age (T02, n=280), and entire males (T03, n=56). From a subgroup of both T01 and T02 and from all pigs of group T03, blood samples were collected immediately before second vaccination (T02) and again before slaughter at either 24 to 25 or 26 to 27 wk of life to determine the plasma concentrations of LH and FSH. Testicles were removed after slaughter and their size was determined. Meat and fat samples from all pigs of T02 and T03 as well as 25% of the pigs of T01 were examined with the cold cooking and fat melting test. Immediately before the second vaccination (T02 only), LH and FSH concentrations were not significantly different between T02 and T03. However, LH and FSH concentrations were significantly higher in T01 compared with T02 and T03. Before the first slaughter date, LH and FSH concentrations were significantly lower in T02 than in T03. Testicle size was significantly lower in T02 compared with that in T03. In T02, 98% (235 of 239) of the samples were rated negative for boar taint by the cooking test, whereas in T03, 94% (48 of 51) were rated positive. In the fat melting test, 97% of T02 were rated negative and 3% (7 pigs) were rated positive, including the pigs tested positive in the cold cooking test. In T03, 94% were rated positive. All pigs (7 of 239) in T02 that were positive for boar taint in the cooking or melting test and that were tested had

  8. Xenotransplantation and pig endogenous retroviruses.

    PubMed

    Magre, Saema; Takeuchi, Yasuhiro; Bartosch, Birke

    2003-01-01

    Xenotransplantation, in particular transplantation of pig cells, tissues and organs into human patients, may alleviate the current shortage of suitable allografts available for human transplantation. This overview addresses the physiological, immunological and virological factors considered with regard to xenotransplantation. Among the issues reviewed are the merits of using pigs as xenograft source species, the compatibility of pig and human organ physiology and the immunological hindrances with regard to the various types of rejection and attempts at abrogating rejection. Advances in the prevention of pig organ rejection by creating genetically modified pigs that are more suited to the human microenvironment are also discussed. Finally, with regard to virology, possible zoonotic infections emanating from pigs are reviewed, with special emphasis on the pig endogenous retrovirus (PERV). An in depth account of PERV studies, comprising their discovery as well as recent knowledge of the virus, is given. To date, all retrospective studies on patients with pig xenografts have shown no evidence of PERV transmission, however, many factors make us interpret these results with caution. Although the lack of PERV infection in xenograft recipients up to now is encouraging, more basic research and controlled animal studies that mimic the pig to human xenotransplantation setting more closely are required for safety assessment.

  9. Increasing Staff Mobility--A Danish Initiative.

    ERIC Educational Resources Information Center

    Sorensen, Henning

    1985-01-01

    Recent Danish government proposals to increase the national and international mobility of scientists are reviewed, including a formalized sabbatical system in the universities, new rules for obtaining leaves of absence with or without salary, and plans for increased mobility between public and private sectors. (MSE)

  10. Care and Education in the Danish Creche

    ERIC Educational Resources Information Center

    Brostrom, Stig; Hansen, Ole Henrik

    2010-01-01

    This article seeks to identify the relation between policy and lived life, for the small child in the Danish creche. To accomplish this, the article integrates demography, traditions, national curriculum and psychological, educational, and recent developments in research. It is an attempt to reveal knowledge and consequences, by conducting the…

  11. The Danish Free School Tradition under Pressure

    ERIC Educational Resources Information Center

    Olsen, Tore Vincents

    2015-01-01

    The Danish free school tradition has entailed a large degree of associational freedom for non-governmental schools, religious as well as non-religious. Until the late 1990s, the non-governmental schools were under no strict ideological or pedagogical limitations; they could recruit teachers and students according to their own value base, and were…

  12. Differential Analysis of the Nasal Microbiome of Pig Carriers or Non-Carriers of Staphylococcus aureus

    PubMed Central

    Espinosa-Gongora, Carmen; Larsen, Niels; Schønning, Kristian; Fredholm, Merete; Guardabassi, Luca

    2016-01-01

    Staphylococcus aureus is presently regarded as an emerging zoonotic agent due to the spread of specific methicillin-resistant S. aureus (MRSA) clones in pig farms. Studying the microbiota can be useful for the identification of bacteria that antagonize such opportunistic veterinary and zoonotic pathogen in animal carriers. The aim of this study was to determine whether the nasal microbiome of pig S. aureus carriers differs from that of non-carriers. The V3-V5 region of the 16S rRNA gene was sequenced from nasal swabs of 44 S. aureus carriers and 56 non-carriers using the 454 GS FLX titanium system. Carriers and non-carriers were selected on the basis of quantitative longitudinal data on S. aureus carriage in 600 pigs sampled at 20 Danish herds included in two previous studies in Denmark. Raw sequences were analysed with the BION meta package and the resulting abundance matrix was analysed using the DESeq2 package in R to identify operational taxonomic units (OTUs) with differential abundance between S. aureus carriers and non-carriers. Twenty OTUs were significantly associated to non-carriers, including species with known probiotic potential and antimicrobial effect such as lactic acid-producing isolates described among Leuconostoc spp. and some members of the Lachnospiraceae family, which is known for butyrate production. Further 5 OTUs were significantly associated to carriage, including known pathogenic bacteria such as Pasteurella multocida and Klebsiella spp. Our results show that the nasal microbiome of pigs that are not colonized with S. aureus harbours several species/taxa that are significantly less abundant in pig carriers, suggesting that the nasal microbiota may play a role in the individual predisposition to S. aureus nasal carriage in pigs. Further research is warranted to isolate these bacteria and assess their possible antagonistic effect on S. aureus for the pursuit of new strategies to control MRSA in pig farming. PMID:27509169

  13. The air quality in Danish urban areas.

    PubMed Central

    Jensen, F P; Fenger, J

    1994-01-01

    The Danish air pollution abatement is based by and large on emission control. Since the ratification of the international sulfur protocol of 1985, there has been a continuous tightening of the permissible sulfur content in fuels and of the maximum emissions from power plants. As a consequence, the total annual emission of sulfur dioxide (SO2) has been reduced from 450,000 tons in the seventies to 180,000 tons in 1990. This has had a pronounced effect on the SO2 levels in Danish urban areas. Thus, in Copenhagen, the yearly averages have fallen to about 25%. For nitrogen oxides emitted from the power plants, similar regulations are in force. With this legislation, the most important and crucial source of air pollution in Danish urban areas is road traffic. The contribution of nitrogen oxides from national traffic accounts for nearly half the total Danish emission and is increasing steadily; this is consistent with an observed increase of nitrogen oxides in ambient air. The permissible levels of lead in petrol has been reduced drastically. After an introduction of reduced tax on lead-free petrol, it now accounts for more than two-thirds of the total consumption. As a result, the concentration of lead in urban ambient air has been reduced to less than one-sixth. The introduction of 3-way catalytic converters from October 1990 will result in reductions in the emission of a series of pollutants, e.g., lead, volatile organic compounds, carbon monoxide, and nitrogen oxides. In 1980, a Danish air quality monitoring program was established as a cooperative effort between the authorities, the Government, the countries, the municipalities, and the Greater Copenhagen Council.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:7821296

  14. The Danish National Multiple Myeloma Registry

    PubMed Central

    Gimsing, Peter; Holmström, Morten O; Klausen, Tobias Wirenfelt; Andersen, Niels Frost; Gregersen, Henrik; Pedersen, Robert Schou; Plesner, Torben; Pedersen, Per Trøllund; Frederiksen, Mikael; Frølund, Ulf; Helleberg, Carsten; Vangsted, Annette; de Nully Brown, Peter; Abildgaard, Niels

    2016-01-01

    Aim The Danish National Multiple Myeloma Registry (DMMR) is a population-based clinical quality database established in January 2005. The primary aim of the database is to ensure that diagnosis and treatment of plasma cell dyscrasia are of uniform quality throughout the country. Another aim is to support research. Patients are registered with their unique Danish personal identification number, and the combined use of DMMR, other Danish National registries, and the Danish National Cancer Biobank offers a unique platform for population-based translational research. Study population All newly diagnosed patients with multiple myeloma (MM), smoldering MM, solitary plasmacytomas, and plasma cell leukemia in Denmark are registered annually; ~350 patients. Amyloid light-chain amyloidosis, POEMS syndrome (polyneuropathy, organomegaly, endocrinopathy, monoclonal gammopathy, and skin changes syndrome), monoclonal gammopathy of undetermined significance and monoclonal gammopathy of undetermined significance with polyneuropathy have been registered since 2014. Main variables The main registered variables at diagnosis are patient demographics, baseline disease characteristics, myeloma-defining events, clinical complications, prognostics, first- and second-line treatments, treatment responses, progression free, and overall survival. Descriptive data Up to June 2015, 2,907 newly diagnosed patients with MM, 485 patients with smoldering MM, 64 patients with plasma cell leukemia, and 191 patients with solitary plasmacytomas were registered. Registration completeness of new patients is ~100%. A data validation study performed in 2013–2014 by the Danish Myeloma Study Group showed >95% data correctness. Conclusion The DMMR is a population-based data validated database eligible for clinical, epidemiological, and translational research. PMID:27822103

  15. Individual Predisposition to Staphylococcus aureus Colonization in Pigs on the Basis of Quantification, Carriage Dynamics, and Serological Profiles

    PubMed Central

    Espinosa-Gongora, Carmen; Dahl, Jan; Elvstrøm, Anders; van Wamel, Willem J.

    2014-01-01

    Previous research on Staphylococcus aureus in pigs focused on livestock-associated methicillin-resistant S. aureus (MRSA) and had a qualitative cross-sectional design. This study aimed to elucidate the frequency, load, and stability of S. aureus nasal carriage in pigs over time and investigated possible associations between carriage and immune response. Nasal swabs were collected three times weekly from 480 tagged adult pigs in 20 Danish production farms. S. aureus and MRSA were quantified on selective media by the most-probable-number method. The levels of IgG against 10 S. aureus antigens in serum were quantified in selected pigs by a Luminex assay. All the farms were positive for S. aureus and 15 for MRSA, leading to overall prevalences of persistent and intermittent carriers and noncarriers of 24, 52, and 23%, respectively. Carriage frequency and nasal loads were significantly higher on MRSA-positive farms. Logistic-regression modeling revealed the presence of individual pigs characterized by high nasal loads (≥10,000 CFU per swab) and stable carriage regardless of farm- and pen-associated factors. On the other hand, the humoral response was strongly influenced by these environmental factors. The existence of a minority of shedders contributing to maintenance of S. aureus within farms opens up new perspectives on the control of MRSA in pig farming. PMID:25501475

  16. Serovars of Salmonella isolated from Danish turkeys between 1995 and 2000 and their antimicrobial resistance.

    PubMed

    Pedersen, K; Hansen, H C; Jørgensen, J C; Borck, B

    2002-04-13

    The prevalence of Salmonella serovars and their antimicrobial resistance patterns were investigated among Danish turkeys between 1995 and 2000, by sampling the flocks approximately 14 days before they were slaughtered. Within the flocks, the prevalence of salmonella varied from 7.1 per cent to 25 per cent, and 24 different serovars were detected. The five most prevalent, which accounted for 58.5 per cent of the isolates were Salmonella Heidelberg (16.2 per cent of the isolates), Salmonella Agona (15.8 per cent), Salmonella Derby (12.4 per cent), Salmonella Muenster (7.3 per cent) and Salmonella Anatum (6.8 per cent). In addition, a few rough isolates and isolates belonging to the antigenically incomplete formulae 6,7:-:- and 4,12:b:- were found. The level of antimicrobial resistance was low; the highest resistance was recorded to ampicillin (13.7 per cent) and streptomycin (9.0 per cent) followed by tetracycline (8.5 per cent), sulphonamides (7.7 per cent) and spectinomycin (4.7 per cent). Resistance to quinolones was very low: four isolates were resistant to nalidixic acid, and only one was resistant to enrofloxacin. No resistance was recorded to colistin, apramycin, ceftiofur, florfenicol, or amoxycillin with clavulanic acid. Only 24 isolates were resistant to two or more compounds in various combinations of up to six compounds; one Salmonella Havana isolate was resistant to six compounds. Six isolates were serovar Typhimurium, but none of them belonged to phage type DT104.

  17. Effect of O. porcinus Tick Salivary Gland Extract on the African Swine Fever Virus Infection in Domestic Pig.

    PubMed

    Bernard, Jennifer; Hutet, Evelyne; Paboeuf, Frédéric; Randriamparany, Tantely; Holzmuller, Philippe; Lancelot, Renaud; Rodrigues, Valérie; Vial, Laurence; Le Potier, Marie-Frédérique

    2016-01-01

    African swine fever is a haemorrhagic disease in pig production that can have disastrous financial consequences for farming. No vaccines are currently available and animal slaughtering or area zoning to restrict risk-related movements are the only effective measures to prevent the spread of the disease. Ornithodoros soft ticks are known to transmit the African swine fever virus (ASFV) to pigs in farms, following the natural epidemiologic cycle of the virus. Tick saliva has been shown to modulate the host physiological and immunological responses during feeding on skin, thus affecting viral infection. To better understand the interaction between soft tick, ASFV and pig at the bite location and the possible influence of tick saliva on pig infection by ASFV, salivary gland extract (SGE) of Ornithodoros porcinus, co-inoculated or not with ASFV, was used for intradermal auricular inoculation. Our results showed that, after the virus triggered the disease, pigs inoculated with virus and SGE presented greater hyperthermia than pigs inoculated with virus alone. The density of Langerhans cells was modulated at the tick bite or inoculation site, either through recruitment by ASFV or inhibition by SGE. Additionally, SGE and virus induced macrophage recruitment each. This effect was enhanced when they were co-inoculated. Finally, the co-inoculation of SGE and virus delayed the early local spread of virus to the first lymph node on the inoculation side. This study has shown that the effect of SGE was powerful enough to be quantified in pig both on the systemic and local immune response. We believe this model should be developed with infected tick and could improve knowledge of both tick vector competence and tick saliva immunomodulation.

  18. Multivariate analysis of traditional pig management practices and their potential impact on the spread of infectious diseases in Corsica.

    PubMed

    Relun, A; Charrier, F; Trabucco, B; Maestrini, O; Molia, S; Chavernac, D; Grosbois, V; Casabianca, F; Etter, E; Jori, F

    2015-10-01

    Corsica is a French Mediterranean island with traditional extensive pig farming oriented towards the production of high quality cured meat products. The increasing success of these cured products in continental Europe has triggered the development and organisation of an extensive pig farming industry. However, these pig farming practices have seldom been described and analysed to understand the potential risk of introduction and spread of infectious diseases. We conducted a cross-sectional study in Corsica in 2013 to characterise the main pig management practices and to identify groups of farms with similar practices and therefore homogeneous risk of introduction and spread of infectious diseases. We interviewed 68 pig farmers and investigated different farm management practices which could lead to contact between herds, such as trading animals, sharing pastures, feed and reproduction management (direct contacts), slaughtering and carcass waste management, and contacts with people and vehicles (indirect contacts). The practices were described and the farms grouped by multiple factor and hierarchical clustering analyses. Results revealed interesting patterns in the introduction and spread of infectious disease, such as the seasonality of pig production, the potential local spread of diseases in pastures due to the presence of free-ranging boars, carcasses, and animal waste. Multivariate analyses identified four groups of farms with different levels of risk of the spread of infectious disease, illustrating changes in farmers' customs from free-range uncontrolled farming systems to more controlled systems aimed at the production of high quality pork products. These results will be useful to more realistically simulate the spread of infectious diseases among Corsican pig farms and highlight the need for awareness raising campaigns among the stakeholders to reduce risky practices.

  19. Effects of feeding pelleted diets without or with distillers dried grains with solubles on fresh belly characteristics, fat quality, and commercial bacon slicing yields of finishing pigs.

    PubMed

    Overholt, M F; Lowell, J E; Wilson, K B; Matulis, R J; Stein, H H; Dilger, A C; Boler, D D

    2016-05-01

    One hundred ninety-two pigs were blocked by age and stratified by initial BW (25.7 ± 2.3 kg) into pens (2 barrows and 2 gilts/pen), and within blocks, pens were assigned randomly to 1 of 4 treatments in a 2 × 2 factorial arrangement, with main effects of diet form (meal vs. pelleted) and distillers dried grains with solubles (DDGS) inclusion (0% vs. 30%). Pigs were slaughtered after a 91-d feeding trial, and carcasses were fabricated after a 24-h chilling period. Belly dimensions and flop distance were measured, and an adipose tissue sample from each belly was collected for fatty acid analysis. Bacon was manufactured at a commercial processing facility before being returned to the University of Illinois Meat Science Laboratory for further evaluation. Although bellies from pigs fed pelleted diets were 5.3% heavier ( < 0.01) than bellies from meal-fed pigs, belly weight as a percentage of chilled side weight ( = 0.55) and fresh belly dimensions ( ≥ 0.11) were not affected by diet form. Slab bacon weight and cooked yield were greater ( ≤ 0.01) for bellies from pellet-fed than meal-fed pigs. Despite pellet-fed pigs having a 3.1-unit greater iodine value (IV) than meal-fed pigs, there was no effect ( ≥ 0.16) of diet form on commercial bacon slicing yields. Bacon slabs from pellet-fed pigs produced more ( < 0.01) total bacon slices, but 3.1% fewer ( < 0.01) slices per kilogram than slabs from meal fed pigs. Inclusion of 30% DDGS reduced belly thickness ( < 0.001), flop distance ( < 0.001), and initial belly weight ( = 0.04) by 0.32 cm, 4.97 cm, and 2.85, respectively, and increased ( < 0.001) belly fat IV by 7.1 units compared with bellies from pigs fed 0% DDGS. Feeding 0% DDGS produced more ( < 0.01) total bacon slices than feeding 30% DDGS. Distillers dried grains with solubles inclusion had no effect on slice yields ( ≥ 0.14) or slices per kilogram ( = 0.08). Overall, bellies from pellet-fed pigs were heavier and had greater IV but did not differ in

  20. Patterns and durations of journeys by horses transported from the USA to Canada for slaughter.

    PubMed

    Roy, R Cyril; Cockram, Michael S

    2015-06-01

    Concern has been expressed over the welfare of horses transported from the USA for slaughter in Canada. United States Department of Agriculture owner/shipper certificates for the year 2009 were analyzed to provide quantitative information on the patterns and durations of these journeys. In 2009, horses from 16 states in the northern USA were transported to 6 equine slaughter plants in Canada. Thirty-two percent of loads were from auction centers, 33% from feedlots, and 35% from horse collection centers. The median duration of the journey was 19 h. Thirty-six percent of horses were transported for < 6 h, 11% for 6 to 18 h, 13% for 18 to 24 h, 25% for 24 to 36 h, 9% for 36 to 48 h, and apparently 6% > 48 h. Some journeys exceeded those specified in regulations and, based on other research, would put these horses at risk of negative welfare outcomes, such as dehydration, injury, and fatigue.

  1. Influence of pre- and post-slaughter factors on the reduced glutathione content of beef muscles.

    PubMed

    Rakowska, R; Sadowska, A; Waszkiewicz-Robak, B

    2017-02-01

    The aim of this experiment was to assess the effect of certain factors (muscle anatomy, paternal breed, diet, age at slaughter, castration, process of meat aging and grilling) on the content of reduced glutathione (GSH) in beef. The research material included selected beef muscles acquired from steers and bulls obtained by crossing Polish Holstein-Friesian cows with meat breed bulls (Limousin, Charolais, Hereford). An analysis of ante-mortem factors such as the castration, slaughter age, and fattening of the animals showed no significant effect on the content of GSH (α=0,05). On the other hand, the paternal breed of animals was observed to have a significant effect on GSH content. In the study, GSH content significantly increased during meat aging. In contrast, grilling caused a loss approximately 40% of GSH content. Based on the study, it can be concluded that the distribution of GSH in anatomical beef muscles is uneven.

  2. Seroprevalence of Neospora caninum in slaughtered native cattle in Kurdistan province, Iran

    PubMed Central

    Heidari, Heidar; Mohammadzadeh, Abdolmajid; Gharekhani, Jamal

    2014-01-01

    Neospora caninum is a worldwide distributed pathogen which causes abortion in cattle leading to economic loss in the cattle industry. The aim of this study was to determine the seroprevalence of N. caninum antibodies in the native cattle slaughtered in various areas of Kurdistan province (western Iran) from September 2010 to September 2011. Serum samples from 368 cattle slaughtered in seven slaughterhouses in this region were taken for detection of anti-N. caninum antibodies using commercial N. caninum ELISA kit. Antibodies to N. caninum were found in 29 samples (7.80%). The present study was the first report of Neospora infection in this region and indicated that native cattle of Kurdistan province were exposed to this parasite. PMID:25568696

  3. Hazard analysis of Escherichia coli O157:H7 contamination during beef slaughtering in Calvados, France.

    PubMed

    Guyon, R; Dorey, F; Malas, J P; Leclercq, A

    2001-09-01

    To identify hazard points and critical points during beef slaughtering, which is a necessary first step toward developing a hazard analysis and critical control point system to control meat contamination by Escherichia coli O157:H7, samples (n = 192) from surfaces, work tops, worker's hands, and beef carcasses were collected from a slaughterhouse in Calvados, France. Five strains of E. coli O157:H7 were isolated from a footbridge and a worker's apron at the preevisceration post and from a worker's hand at the defatting post. Three isolates carried stx2c, eae, and EHEC-hlyA genes and showed similar molecular types by random amplified polymorphic DNA, polymerase chain reaction IS3, and XbaI pulsed-field gel electrophoresis. Thus, this study has shown that preevisceration and defatting post and associated worker's materials are critical points for carcasses contamination by E. coli O157:H7 during beef slaughtering.

  4. Visual evaluation of cattle cleanliness and correlation to carcass microbial contamination during slaughtering.

    PubMed

    Serraino, Andrea; Bardasi, Lia; Riu, Raffaela; Pizzamiglio, Valentina; Liuzzo, Gaetano; Galletti, Giorgio; Giacometti, Federica; Merialdi, Giuseppe

    2012-02-01

    The aim of the study was to establish whether the visual cleanliness of cattle slaughtered was correlated to hide and carcass contamination as indicated by aerobic colony count (ACC), Enterobacteriaceae count (EC) and Escherichia coli count (ECC). Cattle in a slaughterhouse were visually inspected and assigned to a category from 1 (very clean) to 5 (very dirty) based on cleanliness. Fifteen animals for each category were randomly selected, hide and carcass sampled and analyzed for ACC, EC and ECC. Results showed that increasing dirt on cattle was associated with higher ACC, EC and ECC on hide and carcasses. Carcass ACC and ECC belonging to animals classified in cleanliness categories 3, 4 or 5 have a higher probability of exceeding the limits set by the Reg. EU 2073/2005. The study supports the conclusion that the pre-slaughter visual evaluation of animal cleanliness and application of corrective actions can be an effective aid to reduce carcass contamination.

  5. Seroprevalence of Neospora caninum in slaughtered native cattle in Kurdistan province, Iran.

    PubMed

    Heidari, Heidar; Mohammadzadeh, Abdolmajid; Gharekhani, Jamal

    2014-01-01

    Neospora caninum is a worldwide distributed pathogen which causes abortion in cattle leading to economic loss in the cattle industry. The aim of this study was to determine the seroprevalence of N. caninum antibodies in the native cattle slaughtered in various areas of Kurdistan province (western Iran) from September 2010 to September 2011. Serum samples from 368 cattle slaughtered in seven slaughterhouses in this region were taken for detection of anti-N. caninum antibodies using commercial N. caninum ELISA kit. Antibodies to N. caninum were found in 29 samples (7.80%). The present study was the first report of Neospora infection in this region and indicated that native cattle of Kurdistan province were exposed to this parasite.

  6. Effect of pre-slaughter environment on some physiological parameters and meat quality in New Zealand rabbits (Oryctolagus cuniculus).

    PubMed

    Sabuncuoglu, Nilufer; Coban, Omer; Lacin, Ekrem; Ceylan, Ziya Gokalp; Ozdemir, Dervis; Ozkan, Asuman

    2011-02-01

    The objective of this study was to examine the effect of pre-slaughter environment on physiology and meat quality in New Zealand rabbits. Pre-slaughter environment consisted of combined effects of social and non-social factors. For the experiment, 18 male New Zealand rabbits (Oryctolagus cuniculus), 3 months of age, were used. Some biochemical and meat quality parameters of the animals were measured, and the results were compared with the control group. The pre-slaughter environment significantly influenced some biochemical and meat quality parameters (P < 0.01 and P < 0.05, respectively). It is concluded that pre-slaughter environment negatively affected the welfare status and decreased the meat quality in New Zealand rabbits.

  7. Effects of feeding system and slaughter age on the growth and carcass characteristics of tropical-breed steers.

    PubMed

    Agastin, A; Naves, M; Farant, A; Godard, X; Bocage, B; Alexandre, G; Boval, M

    2013-08-01

    This study aimed to compare the growth performances and carcass characteristics of tropical-breed steers reared in 2 contrasted feeding systems (indoor vs. pasture) and slaughtered at different ages (early vs. late). A total of 309 Creole steers (growing at an initial BW of 173 ± 3 kg and an initial age of 252 ± 4 d) were used over a continuous 12-yr study. Indoor steers were housed in a cattle shed, fed fresh-cut grass plus concentrate, and slaughtered at 14.5 or 17.1 ± 0.1 mo of age. Pasture steers were pasture grazed without supplemental feed, and slaughtered at 17.6 and 21.2 ± 0.1 mo of age. Indoor-fed steers had a greater ADG (786 vs. 517 ± 29 g•d(-1); P < 0.0001) and more carcass fat (164 vs. 145 ± 4.5 g•kg(-1); P = 0.001) than pasture-fed steers. Late-slaughtered steers had decreased ADG (630 vs. 673 ± 27 g•d(-1); P = 0.001) but greater dressing percentages (hot dressing percentage = 55.7 vs. 54.7 ± 0.34%; chilled dressing percentage = 54.5 vs. 53.4 ± 0.34%; P < 0.0001) than early-slaughtered steers. The interaction between feeding system and slaughter age was significant for carcass tissue composition. Whole-carcass muscle content was greater in late-slaughtered steers than early-slaughtered steers, especially in pasture-fed steers (720 vs. 698 ± 6.0 g•kg(-1); P < 0.0001), but less so in indoor-fed steers (707 vs. 700 ± 5.9 g•kg(-1); P = 0.046). Furthermore, increasing slaughter age had no effect on carcass fat in indoor-fed steers (162 vs. 166 ± 4.8 g•kg(-1); P = 0.342), but decreased carcass fat in pasture-fed steers (150 vs. 140 ± 5.0 g•kg(-1); P = 0.014). The results showed that slaughter age and feeding system are 2 major factors that independently affect most of the growth and carcass traits of tropical-breed steers but jointly influence tissue deposition. Our study found that in tropical-breed steers that are grazing, late slaughtering grazing steers increased carcass muscle content without extra fat, thus yielding a

  8. Effect of pre-slaughter handling, exercise and the presence of a dog on lamb welfare and meat quality.

    PubMed

    Sutherland, M A; Worth, G M; Stuart, A D; Dobbie, P M; Clerens, S

    2016-08-01

    Before slaughter, lambs may experience several stressors such as feed and water deprivation, handling and transport that have the potential to negatively impact welfare and meat quality. The objective of this study was to evaluate the effect of pre-slaughter handling, exercise and the presence of a dog on the behaviour and physiology of lambs and meat quality at slaughter. At 6 months of age, 60 lambs (n=20 lambs/replicate; three replicates) were allocated to one of the two treatment groups (n=30 lambs/treatment): low (LOW) intensive handling or high (HIGH) intensive handling. LOW lambs were moved short distances, quietly and without the use of a dog before transport. HIGH lambs were moved quickly, long distances and with a dog present before transport. Lamb behaviour (standing, lying, rumination and panting) was recorded for 1 h before (post-treatment) and after transport (post-transport), and for 30 min before slaughter (pre-slaughter). Blood samples were collected before (baseline), after transport (post-transport) and at exsanguination (at slaughter) to assess cortisol, lactate and non-esterified fatty acid (NEFA) concentrations. At slaughter, lamb carcases (M. longissimus lumborum) were evaluated for pH levels, drip and cook loss, and tenderness. HIGH lambs spent more time standing (P<0.001) and panting (P<0.001) and less time lying (P<0.001) and ruminating (P<0.001) post-treatment than LOW lambs, but more (P<0.001) time ruminating post-transport. All lambs spent more time standing (P<0.001) and less time lying (P<0.001) and panting (P<0.001) post-transport and pre-slaughter than post-treatment. Cortisol concentrations were greater (P<0.001) in lambs post-transport and at slaughter compared with baseline values. Lactate concentrations were lower (P=0.002) in HIGH than LOW lambs. In addition, NEFA concentrations were higher (P<0.001) post-transport and at slaughter in HIGH compared with LOW lambs. Ultimate pH was higher (P<0.001) in HIGH than LOW lambs and p

  9. Genetic correlations between visual slaughter conformation scores and growth and reproductive traits in Canchim cattle.

    PubMed

    Borba, L H F; Baldi, F; Feitosa, F L B; da Silva, L O C; Pereira, A S C; Alencar, M M

    2016-05-20

    We obtained heritability and (co)variance component estimates for slaughter conformation scores at 420 days of age (SCS420), age at calving (first, AFC; second, ASC), calving occurrence until 38 months of age (CP38), weight at 420 days of age (W420), and scrotal circumference at 420 days (SC420) in Canchim (5/8 Charolais + 3/8 Zebu) cattle. A total of 23,168 records of Canchim animals, including 12,493 females and 10,675 males, were analyzed. SCS420 indicated carcass structure, muscle development, and subcutaneous fat deposition. The slaughter conformation score of each animal was relative to the whole contemporary group; 1 corresponded to the lowest expression of the trait and 6 to the highest. Heritabilities, and genetic and residual correlation estimates between SCS420 and reproductive and weight traits, were estimated by multitrait analyses using an animal model with Bayesian inference, employing a linear model for AFC, ASC, SC420, and W420 and a threshold model for CP38 and SCS420. Heritability estimates for SCS420, AFC, ASC, CP38, W420, and SC420 were 0.11, 0.15, 0.15, 0.15, 0.30, and 0.30, respectively. Genetic correlation estimates between SCS420 and the other traits were 0.08 (AFC), 0.58 (ASC), 0.08 (CP38), 0.43 (W420), and 0.17 (SC420). Visual slaughter conformation scores respond to individual selection and can be used as selection criteria in Canchim cattle. Selection to improve sexual precocity would not be effective in improving carcass conformation and composition, and selection for animals with high breeding values for yearling weight may improve slaughter conformation at the yearling stage.

  10. Risk Factors for Whole Carcass Condemnations in the Swiss Slaughter Cattle Population

    PubMed Central

    Vial, Flavie; Schärrer, Sara; Reist, Martin

    2015-01-01

    We used meat-inspection data collected over a period of three years in Switzerland to evaluate slaughterhouse-level, farm-level and animal-level factors that may be associated with whole carcass condemnation (WCC) in cattle after slaughter. The objective of this study was to identify WCC risk factors so they can be communicated to, and managed by, the slaughter industry and veterinary services. During meat inspection, there were three main important predictors of the risk of WCC; the slaughtered animal's sex, age, and the size of the slaughterhouse it was processed in. WCC for injuries and significant weight loss (visible welfare indicators) were almost exclusive to smaller slaughterhouses. Cattle exhibiting clinical syndromes that were not externally visible (e.g. pneumonia lesions) and that are associated with fattening of cattle, end up in larger slaughterhouses. For this reason, it is important for animal health surveillance to collect data from both types of slaughterhouses. Other important risk factors for WCC were on-farm mortality rate and the number of cattle on the farm of origin. This study highlights the fact that the many risk factors for WCC are as complex as the production system itself, with risk factors interacting with one another in ways which are sometimes difficult to interpret biologically. Risk-based surveillance aimed at farms with reoccurring health problems (e.g. a history of above average condemnation rates) may be more appropriate than the selection, of higher-risk animals arriving at slaughter. In Switzerland, the introduction of a benchmarking system that would provide feedback to the farmer with information on condemnation reasons, and his/her performance compared to the national/regional average could be a first step towards improving herd-management and financial returns for producers. PMID:25901751

  11. Risk factors for whole carcass condemnations in the Swiss slaughter cattle population.

    PubMed

    Vial, Flavie; Schärrer, Sara; Reist, Martin

    2015-01-01

    We used meat-inspection data collected over a period of three years in Switzerland to evaluate slaughterhouse-level, farm-level and animal-level factors that may be associated with whole carcass condemnation (WCC) in cattle after slaughter. The objective of this study was to identify WCC risk factors so they can be communicated to, and managed by, the slaughter industry and veterinary services. During meat inspection, there were three main important predictors of the risk of WCC; the slaughtered animal's sex, age, and the size of the slaughterhouse it was processed in. WCC for injuries and significant weight loss (visible welfare indicators) were almost exclusive to smaller slaughterhouses. Cattle exhibiting clinical syndromes that were not externally visible (e.g. pneumonia lesions) and that are associated with fattening of cattle, end up in larger slaughterhouses. For this reason, it is important for animal health surveillance to collect data from both types of slaughterhouses. Other important risk factors for WCC were on-farm mortality rate and the number of cattle on the farm of origin. This study highlights the fact that the many risk factors for WCC are as complex as the production system itself, with risk factors interacting with one another in ways which are sometimes difficult to interpret biologically. Risk-based surveillance aimed at farms with reoccurring health problems (e.g. a history of above average condemnation rates) may be more appropriate than the selection, of higher-risk animals arriving at slaughter. In Switzerland, the introduction of a benchmarking system that would provide feedback to the farmer with information on condemnation reasons, and his/her performance compared to the national/regional average could be a first step towards improving herd-management and financial returns for producers.

  12. Salmonella contamination, serovars and antimicrobial resistance profiles of cattle slaughtered in South Africa.

    PubMed

    Madoroba, Evelyn; Kapeta, Daniel; Gelaw, Awoke K

    2016-05-26

    Antimicrobial resistant Salmonella are among the leading causes of foodborne infections. Our aim was to determine Salmonella contamination during cattle slaughter in South African rural abattoirs (n = 23) and environmental samples. Furthermore, antimicrobial resistance patterns of the Salmonella isolates were determined. Samples of cattle faeces (n = 400), carcass sponges (n = 100), intestinal contents (n = 62), hides (n = 67), and water from the abattoirs (n = 75) were investigated for Salmonella species using microbiological techniques and species-specific polymerase chain reaction targeting the invA gene. In total 92 Salmonella species isolates were recovered. The Salmonella mean frequency of occurrence on hides, carcasses, and intestinal contents was 35.37% (n = 81). Eleven faecal samples (2.75%) tested positive for Salmonella. The predominant serovar was Salmonella Enteritidis. Diverse serovars that were identified on carcasses were not necessarily found on the hides and intestinal contents. The inconsistent occurrence of the diverse Salmonella serovars on hides, carcasses, and intestinal contents implies that in addition to carriage on hides and in intestinal contents, other external factors also play an important role regarding carcass contamination. The 92 Salmonella were serotyped and tested for susceptibility towards the following antimicrobials: ampicillin, cefotaxime, enrofloxacin, kanamycin, and oxytetracycline using the disk diffusion method. Most Salmonella (n = 66; 71.7%) isolates were resistant to at least one antimicrobial with highest resistance observed towards oxytetracycline (51.90%), which highlights the need for strict hygiene during slaughter and prudent antimicrobial use during animal production. In conclusion, cattle slaughtered in South African rural abattoirs harbour diverse Salmonella serovars that are resistant to antimicrobials, which could be a public health risk. The findings should assist policymakers with improving implementation

  13. Salmonella contamination, serovars and antimicrobial resistance profiles of cattle slaughtered in South Africa.

    PubMed

    Madoroba, Evelyn; Kapeta, Daniel; Gelaw, Awoke K

    2016-05-26

    Antimicrobial resistant Salmonella are among the leading causes of foodborne infections. Our aim was to determine Salmonella contamination during cattle slaughter in South African rural abattoirs (n = 23) and environmental samples. Furthermore, antimicrobial resistance patterns of the Salmonella isolates were determined. Samples of cattle faeces (n = 400), carcass sponges (n = 100), intestinal contents (n = 62), hides (n = 67), and water from the abattoirs (n = 75) were investigated for Salmonella species using microbiological techniques and species-specific polymerase chain reaction targeting the invA gene. In total 92 Salmonella species isolates were recovered. The Salmonella mean frequency of occurrence on hides, carcasses, and intestinal contents was 35.37% (n = 81). Eleven faecal samples (2.75%) tested positive for Salmonella. The predominant serovar was Salmonella Enteritidis. Diverse serovars that were identified on carcasses were not necessarily found on the hides and intestinal contents. The inconsistent occurrence of the diverse Salmonella serovars on hides, carcasses, and intestinal contents implies that in addition to carriage on hides and in intestinal contents, other external factors also play an important role regarding carcass contamination. The 92 Salmonella were serotyped and tested for susceptibility towards the following antimicrobials: ampicillin, cefotaxime, enrofloxacin, kanamycin, and oxytetracycline using the disk diffusion method. Most Salmonella (n = 66; 71.7%) isolates were resistant to at least one antimicrobial with highest resistance observed towards oxytetracycline (51.90%), which highlights the need for strict hygiene during slaughter and prudent antimicrobial use during animal production. In conclusion, cattle slaughtered in South African rural abattoirs harbour diverse Salmonella serovars that are resistant to antimicrobials, which could be a public health risk. The findings should assist policymakers with improving implementation

  14. Transcript profiling of expressed sequence tags from semimembranosus muscle of commercial and naturalized pig breeds.

    PubMed

    Nascimento, C S; Peixoto, J O; Verardo, L L; Campos, C F; Weller, M M C; Faria, V R; Botelho, M E; Martins, M F; Machado, M A; Silva, F F; Lopes, P S; Guimarães, S E F

    2012-09-17

    In general, genetic differences across different breeds of pig lead to variation in mature body size and slaughter age. The Commercial breeds Duroc and Large White and the local Brazilian breed Piau are ostensibly distinct in terms of growth and muscularity, commercial breeds are much leaner while local breeds grow much slower and are fat type pigs. However, the genetic factors that underlie such distinctions remain unclear. We used expressed sequence tags (ESTs) to characterize and compare transcript profiles in the semimembranosus muscle of these pig breeds. Our aim was to identify differences in breed-related gene expression that might influence growth performance and meat quality. We constructed three non-normalized cDNA libraries from semimembranosus muscle, using two samples from each one, of these three breeds; 6902 high-quality ESTs were obtained. Cluster analysis was performed and these sequences were clustered into 3670 unique sequences; 24.7% of the sequences were categorized as contigs and 75.3% of the sequences were singletons. Based on homology searches against the SwissProt protein database, we were able to assign a putative protein identity to only 1050 unique sequences. Among these, 58.5% were full-length protein sequences and 17.2% were pig-specific sequences. Muscle structural and cytoskeletal proteins, such as actin, and myosin, were the most abundant transcripts (16.7%) followed by those related to mitochondrial function (12.9%), and ribosomal proteins (12.4%). Furthermore, ESTs generated in this study provide a rich source for identification of novel genes and for the comparative analysis of gene expression patterns in divergent pig breeds.

  15. Some Gastrointestinal Tract Characteristics of Karayaka Ram Lambs Slaughtered at Different Weights

    PubMed Central

    Yıldırım, Arda; Aksoy, Yüksel; Ocak, Nuh; Ulutaş, Zafer

    2014-01-01

    Thirty-one Karayaka ram lambs were slaughtered at different body weights (30 (n  =  7) , 35 (n  =  6) , 40 (n  =  7) , 45 (n  =  6) , and 50 (n  =  5)  kg of body weight at fast) to evaluate the growth of their gastrointestinal tract (GIT) characteristics, to determine the relationship among slaughter body weight (SBW) and empty body weight (EBW), whole GIT and segments, and the influence of slaughter weight on the pH of rumen, jejunum, and cecal contents. The effects of the SBW on GIT weight (P  <  0.05) , stomach ( P  <  0.001) , and intestine (P  <  0.05) , the body length (P  <  0.001) and caecum (P  <  0.05) , and the relative weights of GIT (P  <  0.05) , stomach (P <  0.001) , and intestine (P  <  0.001) were linear whereas that for the length of intestine were quadratic. The effect of SBW were quadratic (P  <  0.05) on ratios of stomach to GIT weight and intestine length to intestine weight and rumen pH while, for the intestine to GIT weight ratio (P  <  0.001) and caecum pH (P  <  0.05) , this effect was linear. The results indicated that for all parameters studied, with the exception of intestinal length and cecal pH, linear relationships were observed with SBW indicating steady growth rates for these tissues. PMID:25133226

  16. Breed, slaughter weight and ageing time effects on sensory characteristics of lamb.

    PubMed

    Martínez-Cerezo, S; Sañudo, C; Medel, I; Olleta, J L

    2005-03-01

    The longissimus lumborum (right and left) from 180 entire male lambs were tasted by a nine-member trained taste panel. The samples were from lambs from three Spanish breeds; Rasa Aragonesa (local meat breed), Churra (local dairy breed) and Spanish Merino. Within breed, three slaughter live weights were considered (10-12, 20-22 or 30-32 kg) and meat was aged in a vacuum package for 1, 2, 4, 8 or 16 days. Panellists assessed a total of eight descriptors. Lamb odour and lamb flavour intensities increased with slaughter weight. However, fat flavour intensity was significantly influenced by breed, with the highest score for Rasa Aragonesa. Off-flavour intensity was influenced by breed and ageing, with higher scores for Rasa Aragonesa and longer ageing times. Tenderness and juiciness were influenced by all three effects (P⩽0.001, each) and there was a significant interaction between breed and slaughter live weight. Meat was juicier and more tender in the lightest Churra lambs, and in the Spanish Merino lambs for the heavier weight animals. Tenderness and juiciness increased with ageing. The best quality flavour was for the Spanish Merino and the intermediate and heavier lambs.

  17. Transport versus on-farm slaughter of bison: physiological stress, animal welfare, and avoidable trim losses.

    PubMed

    McCorkell, Robert; Wynne-Edwards, Katherine; Galbraith, Jayson; Schaefer, Al; Caulkett, Nigel; Boysen, Soren; Pajor, Ed

    2013-08-01

    Ranched bison are typically less acclimated to handling than are domesticated livestock, suggesting that they might be more vulnerable to handling and transportation stressors. Grain-finished bison were slaughtered on-farm (n = 11), or held for 48 h, transported to a research abattoir, held in lairage for 18 h, and then slaughtered (n = 11). An additional group (n = 10) was sampled at a conventional fixed location abattoir. Measures included serum cortisol and corticosterone concentrations during on-farm handling and exsanguination, serum glucose, creatinine phosphokinase (CPK), aspartate aminotransferase (AST), and trim losses. Transport was associated with an increase in trim loss. On-farm, glucose was elevated, CPK was positively associated with handling order over 12 h, and corticosterone concentration, although lower than cortisol concentration, showed a greater response to prolonged disturbance. With appropriate on-farm handling facilities, the use of on-farm slaughter and mobile abattoir could avoid muscle damage and trim losses, and mitigate injuries sustained during handling and transport of bison.

  18. Genotypic and antimicrobial characterization of pathogenic bacteria at different stages of cattle slaughtering in southern Brazil.

    PubMed

    L