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Sample records for darmstadtium 271

  1. 32. LOOKING NORTHEAST DOWN WALKWAY CONNECTING BUILDING NO.S 271, 271G, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    32. LOOKING NORTHEAST DOWN WALKWAY CONNECTING BUILDING NO.S 271, 271-G, 271-I, 271-L, 271-K, ETC. MIRRORS IN UPPER RIGHT PERMIT WORKERS TO SEE AROUND CORNER TO CORRIDOR LEADING TO BUILDING NO. 271-H (LEAD AZIDE PREPARATION BUILDING). - Picatinny Arsenal, 200 Area, Shell Component Loading, State Route 15 near I-80, Dover, Morris County, NJ

  2. 30. BUILDING NO.S 271K AND 271L, VIEW LOOKING SOUTH AT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    30. BUILDING NO.S 271-K AND 271-L, VIEW LOOKING SOUTH AT BACK OF BUILDING NO. 271-L (LEFT), 271-K (MIDDLE) AND ROOF OF BUILDING NO. 271-I (VISIBLE OVER WALKWAY ON RIGHT). - Picatinny Arsenal, 200 Area, Shell Component Loading, State Route 15 near I-80, Dover, Morris County, NJ

  3. 40 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 26 2010-07-01 2010-07-01 false Definitions. 271.2 Section 271.2 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.2...

  4. 4 CFR 27.1 - The Board.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 4 Accounts 1 2014-01-01 2013-01-01 true The Board. 27.1 Section 27.1 Accounts GOVERNMENT... § 27.1 The Board. The Government Accountability Office Personnel Appeals Board, hereinafter the Board, is composed of five members appointed by the Comptroller General, in accordance with the provisions...

  5. 4 CFR 27.1 - The Board.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 4 Accounts 1 2013-01-01 2013-01-01 false The Board. 27.1 Section 27.1 Accounts GOVERNMENT... § 27.1 The Board. The Government Accountability Office Personnel Appeals Board, hereinafter the Board, is composed of five members appointed by the Comptroller General, in accordance with the provisions...

  6. 4 CFR 27.1 - The Board.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 4 Accounts 1 2012-01-01 2012-01-01 false The Board. 27.1 Section 27.1 Accounts GOVERNMENT... § 27.1 The Board. The Government Accountability Office Personnel Appeals Board, hereinafter the Board, is composed of five members appointed by the Comptroller General, in accordance with the provisions...

  7. 4 CFR 27.1 - The Board.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 4 Accounts 1 2011-01-01 2011-01-01 false The Board. 27.1 Section 27.1 Accounts GOVERNMENT... § 27.1 The Board. The Government Accountability Office Personnel Appeals Board, hereinafter the Board, is composed of five members appointed by the Comptroller General, in accordance with the provisions...

  8. 33 CFR 117.271 - Blackwater River.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 1 2014-07-01 2014-07-01 false Blackwater River. 117.271 Section 117.271 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Florida § 117.271 Blackwater River. The draw of the...

  9. 33 CFR 117.271 - Blackwater River.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Blackwater River. 117.271 Section 117.271 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Florida § 117.271 Blackwater River. The draw of the...

  10. 33 CFR 117.271 - Blackwater River.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Blackwater River. 117.271 Section 117.271 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Florida § 117.271 Blackwater River. The draw of the...

  11. 33 CFR 117.271 - Blackwater River.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 1 2013-07-01 2013-07-01 false Blackwater River. 117.271 Section 117.271 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Florida § 117.271 Blackwater River. The draw of the...

  12. 33 CFR 117.271 - Blackwater River.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 1 2012-07-01 2012-07-01 false Blackwater River. 117.271 Section 117.271 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Florida § 117.271 Blackwater River. The draw of the...

  13. 4 CFR 27.1 - The Board.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 4 Accounts 1 2010-01-01 2010-01-01 false The Board. 27.1 Section 27.1 Accounts GOVERNMENT... § 27.1 The Board. The Government Accountability Office Personnel Appeals Board, hereinafter the Board, is composed of five members appointed by the Comptroller General, in accordance with the provisions...

  14. 36 CFR 271.5 - [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 36 Parks, Forests, and Public Property 2 2011-07-01 2011-07-01 false 271.5 Section 271.5 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE USE OF âSMOKEY BEARâ SYMBOL § 271.5 ...

  15. 36 CFR 271.1 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Definitions. 271.1 Section 271.1 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE USE OF âSMOKEY BEARâ SYMBOL § 271.1 Definitions. (a) The term Smokey Bear as used in the regulations in this part means...

  16. 36 CFR 271.5 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false 271.5 Section 271.5 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE USE OF âSMOKEY BEARâ SYMBOL § 271.5 ...

  17. 36 CFR 271.1 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 36 Parks, Forests, and Public Property 2 2011-07-01 2011-07-01 false Definitions. 271.1 Section 271.1 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE USE OF âSMOKEY BEARâ SYMBOL § 271.1 Definitions. (a) The term Smokey Bear as used in the regulations in this part means...

  18. 14 CFR 271.4 - Carrier costs.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Carrier costs. 271.4 Section 271.4 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.4...

  19. 46 CFR 107.271 - Inspection: Alterations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Inspection: Alterations. 107.271 Section 107.271 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS INSPECTION AND CERTIFICATION Inspection and Certification § 107.271 Inspection: Alterations. After plans are...

  20. 46 CFR 107.271 - Inspection: Alterations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Inspection: Alterations. 107.271 Section 107.271 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS INSPECTION AND CERTIFICATION Inspection and Certification § 107.271 Inspection: Alterations. After plans are...

  1. 46 CFR 107.271 - Inspection: Alterations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Inspection: Alterations. 107.271 Section 107.271 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS INSPECTION AND CERTIFICATION Inspection and Certification § 107.271 Inspection: Alterations. After plans are...

  2. 46 CFR 107.271 - Inspection: Alterations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Inspection: Alterations. 107.271 Section 107.271 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS INSPECTION AND CERTIFICATION Inspection and Certification § 107.271 Inspection: Alterations. After plans are...

  3. 46 CFR 107.271 - Inspection: Alterations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Inspection: Alterations. 107.271 Section 107.271 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS INSPECTION AND CERTIFICATION Inspection and Certification § 107.271 Inspection: Alterations. After plans are...

  4. 48 CFR 1352.271-80 - Title.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Title. 1352.271-80 Section 1352.271-80 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-80 Title. As prescribed in 48 CFR 1371.111, insert the following...

  5. 48 CFR 1352.271-80 - Title.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Title. 1352.271-80 Section 1352.271-80 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-80 Title. As prescribed in 48 CFR 1371.111, insert the following...

  6. 48 CFR 1352.271-80 - Title.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Title. 1352.271-80 Section 1352.271-80 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-80 Title. As prescribed in 48 CFR 1371.111, insert the following...

  7. 48 CFR 1352.271-80 - Title.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Title. 1352.271-80 Section 1352.271-80 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-80 Title. As prescribed in 48 CFR 1371.111, insert the following...

  8. 12 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Definitions. 271.2 Section 271.2 Banks and... INFORMATION § 271.2 Definitions. (a) Board means the Board of Governors of the Federal Reserve System established by the Federal Reserve Act of 1913 (38 Stat. 251). (b) Commercial use request refers to a request...

  9. 7 CFR 1150.271 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 9 2012-01-01 2012-01-01 false Definitions. 1150.271 Section 1150.271 Agriculture... Milk Producer Organizations § 1150.271 Definitions. As used in this subpart: (a) Act means Title I, Subtitle B, of the Dairy and Tobacco Adjustment Act of 1983, Pub. L. 98-180, 97 Stat. 1128, as approved...

  10. 12 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 12 Banks and Banking 4 2014-01-01 2014-01-01 false Definitions. 271.2 Section 271.2 Banks and... INFORMATION § 271.2 Definitions. (a) Board means the Board of Governors of the Federal Reserve System established by the Federal Reserve Act of 1913 (38 Stat. 251). (b) Commercial use request refers to a request...

  11. 7 CFR 1150.271 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 9 2011-01-01 2011-01-01 false Definitions. 1150.271 Section 1150.271 Agriculture... Milk Producer Organizations § 1150.271 Definitions. As used in this subpart: (a) Act means Title I, Subtitle B, of the Dairy and Tobacco Adjustment Act of 1983, Pub. L. 98-180, 97 Stat. 1128, as approved...

  12. 7 CFR 1150.271 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 9 2013-01-01 2013-01-01 false Definitions. 1150.271 Section 1150.271 Agriculture... Milk Producer Organizations § 1150.271 Definitions. As used in this subpart: (a) Act means Title I, Subtitle B, of the Dairy and Tobacco Adjustment Act of 1983, Pub. L. 98-180, 97 Stat. 1128, as approved...

  13. 27 CFR 25.271 - General.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2011-04-01 2011-04-01 false General. 25.271 Section 25.271 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS BEER Pilot Brewing Plants § 25.271 General. (a) Establishment. A person may establish and...

  14. 27 CFR 25.271 - General.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2013-04-01 2013-04-01 false General. 25.271 Section 25.271 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY ALCOHOL BEER Pilot Brewing Plants § 25.271 General. (a) Establishment. A person may establish and...

  15. 7 CFR 1150.271 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 9 2014-01-01 2013-01-01 true Definitions. 1150.271 Section 1150.271 Agriculture... Milk Producer Organizations § 1150.271 Definitions. As used in this subpart: (a) Act means Title I, Subtitle B, of the Dairy and Tobacco Adjustment Act of 1983, Pub. L. 98-180, 97 Stat. 1128, as approved...

  16. 27 CFR 25.271 - General.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2014-04-01 2014-04-01 false General. 25.271 Section 25.271 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY ALCOHOL BEER Pilot Brewing Plants § 25.271 General. (a) Establishment. A person may establish and...

  17. 12 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 12 Banks and Banking 4 2012-01-01 2012-01-01 false Definitions. 271.2 Section 271.2 Banks and... INFORMATION § 271.2 Definitions. (a) Board means the Board of Governors of the Federal Reserve System established by the Federal Reserve Act of 1913 (38 Stat. 251). (b) Commercial use request refers to a request...

  18. 27 CFR 25.271 - General.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2012-04-01 2012-04-01 false General. 25.271 Section 25.271 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS BEER Pilot Brewing Plants § 25.271 General. (a) Establishment. A person may establish and...

  19. 43 CFR 27.1 - Purpose.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 43 Public Lands: Interior 1 2014-10-01 2014-10-01 false Purpose. 27.1 Section 27.1 Public Lands: Interior Office of the Secretary of the Interior NONDISCRIMINATION IN ACTIVITIES CONDUCTED UNDER PERMITS... PUBLIC LAW 93-153 § 27.1 Purpose. The purpose of this part is to effectuate section 403 of Public Law...

  20. 48 CFR 852.271-71 - [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false 852.271-71 Section 852.271-71 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Texts of Provisions and Clauses 852.271-71...

  1. 48 CFR 852.271-74 - Inspection.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Inspection. 852.271-74 Section 852.271-74 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Texts of Provisions and Clauses 852.271-74 Inspection....

  2. 48 CFR 1352.271-80 - Title.

    Code of Federal Regulations, 2010 CFR

    2016-10-01

    ... 48 Federal Acquisition Regulations System 5 2016-10-01 2016-10-01 false Title. 1352.271-80 Section 1352.271-80 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-80 Title. As prescribed in 48 CFR 1371.111, insert the following...

  3. 43 CFR 27.1 - Purpose.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Purpose. 27.1 Section 27.1 Public Lands..., RIGHTS-OF-WAY, PUBLIC LAND ORDERS, AND OTHER FEDERAL AUTHORIZATIONS GRANTED OR ISSUED UNDER TITLE II OF PUBLIC LAW 93-153 § 27.1 Purpose. The purpose of this part is to effectuate section 403 of Public Law...

  4. 48 CFR 1352.271-80 - Title.

    Code of Federal Regulations, 2010 CFR

    2012-10-01

    ... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-80 Title. As prescribed in 48 CFR 1371.111, insert the following clause: Title (APR 2010) (a) Title to all materials and... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Title. 1352.271-80...

  5. 48 CFR 1352.271-80 - Title.

    Code of Federal Regulations, 2010 CFR

    2015-10-01

    ... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-80 Title. As prescribed in 48 CFR 1371.111, insert the following clause: Title (APR 2010) (a) Title to all materials and... 48 Federal Acquisition Regulations System 5 2015-10-01 2015-10-01 false Title. 1352.271-80...

  6. 40 CFR 264.271 - Treatment program.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Treatment program. 264.271 Section 264...) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.271 Treatment program. (a) An owner or operator subject to this subpart must establish...

  7. 48 CFR 247.271-1 - Policy.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 3 2014-10-01 2014-10-01 false Policy. 247.271-1 Section 247.271-1 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF... volume; (B) Size of overall area; and (C) The need to service isolated areas of high population density...

  8. 14 CFR 271.9 - Discrimination prohibited.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 4 2014-01-01 2014-01-01 false Discrimination prohibited. 271.9 Section... TRANSPORTATION § 271.9 Discrimination prohibited. (a) All air carriers receiving subsidy under this part shall comply with the following: (1) The Age Discrimination Act of 1975; (2) The Civil Rights Act of 1964 and...

  9. 14 CFR 271.9 - Discrimination prohibited.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 4 2012-01-01 2012-01-01 false Discrimination prohibited. 271.9 Section... TRANSPORTATION § 271.9 Discrimination prohibited. (a) All air carriers receiving subsidy under this part shall comply with the following: (1) The Age Discrimination Act of 1975; (2) The Civil Rights Act of 1964 and...

  10. 14 CFR 271.9 - Discrimination prohibited.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false Discrimination prohibited. 271.9 Section... TRANSPORTATION § 271.9 Discrimination prohibited. (a) All air carriers receiving subsidy under this part shall comply with the following: (1) The Age Discrimination Act of 1975; (2) The Civil Rights Act of 1964 and...

  11. 14 CFR 271.9 - Discrimination prohibited.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Discrimination prohibited. 271.9 Section... TRANSPORTATION § 271.9 Discrimination prohibited. (a) All air carriers receiving subsidy under this part shall comply with the following: (1) The Age Discrimination Act of 1975; (2) The Civil Rights Act of 1964 and...

  12. 14 CFR 171.271 - Installation requirements.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Installation requirements. 171.271 Section 171.271 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) NAVIGATIONAL FACILITIES NON-FEDERAL NAVIGATION FACILITIES Interim Standard Microwave Landing System (ISMLS...

  13. 14 CFR 271.9 - Discrimination prohibited.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Discrimination prohibited. 271.9 Section... TRANSPORTATION § 271.9 Discrimination prohibited. (a) All air carriers receiving subsidy under this part shall comply with the following: (1) The Age Discrimination Act of 1975; (2) The Civil Rights Act of 1964...

  14. 48 CFR 1842.271 - NASA clause.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 6 2013-10-01 2013-10-01 false NASA clause. 1842.271 Section 1842.271 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE ADMINISTRATION... NASA clause. Insert the clause at 1852.242-70, Technical Direction, when paragraph 3(m) of the NASA...

  15. 48 CFR 1842.271 - NASA clause.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 6 2011-10-01 2011-10-01 false NASA clause. 1842.271 Section 1842.271 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE ADMINISTRATION... NASA clause. Insert the clause at 1852.242-70, Technical Direction, when paragraph 3(m) of the NASA...

  16. 48 CFR 1842.271 - NASA clause.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true NASA clause. 1842.271 Section 1842.271 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE ADMINISTRATION... NASA clause. Insert the clause at 1852.242-70, Technical Direction, when paragraph 3(m) of the NASA...

  17. 48 CFR 1842.271 - NASA clause.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 6 2012-10-01 2012-10-01 false NASA clause. 1842.271 Section 1842.271 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE ADMINISTRATION... NASA clause. Insert the clause at 1852.242-70, Technical Direction, when paragraph 3(m) of the NASA...

  18. 48 CFR 1842.271 - NASA clause.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 6 2014-10-01 2014-10-01 false NASA clause. 1842.271 Section 1842.271 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE ADMINISTRATION... NASA clause. Insert the clause at 1852.242-70, Technical Direction, when paragraph 3(m) of the NASA...

  19. 36 CFR 271.4 - Commercial license.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 36 Parks, Forests, and Public Property 2 2011-07-01 2011-07-01 false Commercial license. 271.4... BEARâ SYMBOL § 271.4 Commercial license. (a) The Chief may authorize the commercial manufacture... a use or royalty charge which is reasonably related to the commercial enterprise has...

  20. 14 CFR 271.6 - Profit element.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 4 2014-01-01 2014-01-01 false Profit element. 271.6 Section 271.6 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC... element. The reasonable return for a carrier for providing essential air service at an eligible place...

  1. 14 CFR 271.6 - Profit element.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Profit element. 271.6 Section 271.6 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC... element. The reasonable return for a carrier for providing essential air service at an eligible place...

  2. 14 CFR 271.6 - Profit element.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false Profit element. 271.6 Section 271.6 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC... element. The reasonable return for a carrier for providing essential air service at an eligible place...

  3. 14 CFR 271.6 - Profit element.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 4 2012-01-01 2012-01-01 false Profit element. 271.6 Section 271.6 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC... element. The reasonable return for a carrier for providing essential air service at an eligible place...

  4. 48 CFR 1352.271-88 - Guarantees.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Guarantees. 1352.271-88 Section 1352.271-88 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND FORMS... and at its own expense, shall correct and repair the deficiency to the satisfaction of the...

  5. 48 CFR 1352.271-88 - Guarantees.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Guarantees. 1352.271-88 Section 1352.271-88 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND FORMS... and at its own expense, shall correct and repair the deficiency to the satisfaction of the...

  6. 48 CFR 1352.271-88 - Guarantees.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Guarantees. 1352.271-88 Section 1352.271-88 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND FORMS... and at its own expense, shall correct and repair the deficiency to the satisfaction of the...

  7. 40 CFR 264.271 - Treatment program.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 27 2013-07-01 2013-07-01 false Treatment program. 264.271 Section 264...) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.271 Treatment program. (a) An owner or operator subject to this subpart must establish a...

  8. 40 CFR 264.271 - Treatment program.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false Treatment program. 264.271 Section 264...) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.271 Treatment program. (a) An owner or operator subject to this subpart must establish a...

  9. 40 CFR 264.271 - Treatment program.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Treatment program. 264.271 Section 264...) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.271 Treatment program. (a) An owner or operator subject to this subpart must establish a...

  10. 40 CFR 264.271 - Treatment program.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false Treatment program. 264.271 Section 264...) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Land Treatment § 264.271 Treatment program. (a) An owner or operator subject to this subpart must establish a...

  11. 48 CFR 1352.271-76 - Performance.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Performance. 1352.271-76... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-76 Performance. As prescribed in 48 CFR 1371.107, insert the following clause: Performance (APR 2010) (a) The contractor...

  12. 48 CFR 719.271-1 - General.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 719.271-1 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-1 General. The purpose of this section is to prescribe responsibilities and procedures for carrying out the small business program policy set forth in 219.270, and...

  13. 12 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 12 Banks and Banking 4 2013-01-01 2013-01-01 false Definitions. 271.2 Section 271.2 Banks and... private elementary or secondary school, or an institution of undergraduate higher education, graduate higher education, professional education, or an institution of vocational education that operates...

  14. 12 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 3 2011-01-01 2011-01-01 false Definitions. 271.2 Section 271.2 Banks and... private elementary or secondary school, or an institution of undergraduate higher education, graduate higher education, professional education, or an institution of vocational education that operates...

  15. 40 CFR 271.25 - HSWA requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ....25 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.25... Solid Waste Amendments of 1984 (HSWA). ...

  16. 30 CFR 27.1 - Purpose.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... PRODUCTS METHANE-MONITORING SYSTEMS General Provisions § 27.1 Purpose. The regulations in this part set forth the requirements for methane-monitoring systems or components thereof to procure certification...

  17. 14 CFR 271.8 - Rate period.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.8 Rate period... place essential air service level; or (5) The uncertainties of the market or other circumstances warrant... essential air service at the eligible place. ...

  18. 14 CFR 271.5 - Carrier revenues.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.5 Carrier revenues. (a) The projected passenger revenue for a carrier providing essential air service at an eligible...

  19. 14 CFR 271.6 - Profit element.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.6 Profit element. The reasonable return for a carrier for providing essential air service at an eligible place...

  20. 14 CFR 271.1 - Purpose.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.1 Purpose... establishing the fair and reasonable amount of compensation needed to ensure the continuation of essential air...

  1. 30 CFR 27.1 - Purpose.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... PRODUCTS METHANE-MONITORING SYSTEMS General Provisions § 27.1 Purpose. The regulations in this part set forth the requirements for methane-monitoring systems or components thereof to procure certification...

  2. 30 CFR 27.1 - Purpose.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... PRODUCTS METHANE-MONITORING SYSTEMS General Provisions § 27.1 Purpose. The regulations in this part set forth the requirements for methane-monitoring systems or components thereof to procure certification for...

  3. 30 CFR 27.1 - Purpose.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... PRODUCTS METHANE-MONITORING SYSTEMS General Provisions § 27.1 Purpose. The regulations in this part set forth the requirements for methane-monitoring systems or components thereof to procure certification for...

  4. 30 CFR 27.1 - Purpose.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... PRODUCTS METHANE-MONITORING SYSTEMS General Provisions § 27.1 Purpose. The regulations in this part set forth the requirements for methane-monitoring systems or components thereof to procure certification for...

  5. 48 CFR 1516.405-271 - Limitations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... METHODS AND CONTRACT TYPES TYPES OF CONTRACTS Incentive Contracts 1516.405-271 Limitations. (a) No award... satisfactory or excellent. All award fee plans shall disclose to offerors the numerical rating necessary to...

  6. 40 CFR 98.271 - Reporting threshold.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...) MANDATORY GREENHOUSE GAS REPORTING Pulp and Paper Manufacturing § 98.271 Reporting threshold. You must report GHG emissions under this subpart if your facility contains a pulp and paper manufacturing process...

  7. 40 CFR 98.271 - Reporting threshold.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...) MANDATORY GREENHOUSE GAS REPORTING Pulp and Paper Manufacturing § 98.271 Reporting threshold. You must report GHG emissions under this subpart if your facility contains a pulp and paper manufacturing process...

  8. 40 CFR 98.271 - Reporting threshold.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...) MANDATORY GREENHOUSE GAS REPORTING Pulp and Paper Manufacturing § 98.271 Reporting threshold. You must report GHG emissions under this subpart if your facility contains a pulp and paper manufacturing process...

  9. 40 CFR 98.271 - Reporting threshold.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...) MANDATORY GREENHOUSE GAS REPORTING Pulp and Paper Manufacturing § 98.271 Reporting threshold. You must report GHG emissions under this subpart if your facility contains a pulp and paper manufacturing process...

  10. 40 CFR 98.271 - Reporting threshold.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...) MANDATORY GREENHOUSE GAS REPORTING Pulp and Paper Manufacturing § 98.271 Reporting threshold. You must report GHG emissions under this subpart if your facility contains a pulp and paper manufacturing process...

  11. 40 CFR 271.20 - Approval process.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ....20 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.20... program submission for final authorization, the Administrator shall make a tentative determination as to...

  12. 40 CFR 271.6 - Program description.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ....6 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.6... its program. Forms used by the State for hazardous waste management need not be identical to the forms...

  13. 40 CFR 271.4 - Consistency.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.4... hazardous waste programs which have received final authorization under this part. (a) Any aspect of the...

  14. 40 CFR 61.271 - Emission standard.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS National Emission Standard for Benzene Emissions From Benzene Storage Vessels § 61.271 Emission standard. The owner or operator of each storage vessel... vent system shall be designed to collect all benzene vapors and gases discharged from the...

  15. 40 CFR 61.271 - Emission standard.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS National Emission Standard for Benzene Emissions From Benzene Storage Vessels § 61.271 Emission standard. The owner or operator of each storage vessel... vent system shall be designed to collect all benzene vapors and gases discharged from the...

  16. 40 CFR 61.271 - Emission standard.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS National Emission Standard for Benzene Emissions From Benzene Storage Vessels § 61.271 Emission standard. The owner or operator of each storage vessel... vent system shall be designed to collect all benzene vapors and gases discharged from the...

  17. 40 CFR 61.271 - Emission standard.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS National Emission Standard for Benzene Emissions From Benzene Storage Vessels § 61.271 Emission standard. The owner or operator of each storage vessel... vent system shall be designed to collect all benzene vapors and gases discharged from the...

  18. 27 CFR 25.271 - General.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... TREASURY LIQUORS BEER Pilot Brewing Plants § 25.271 General. (a) Establishment. A person may establish and... developmental purposes relating to beer or brewery operations. Pilot brewing plants will be established as provided in this subpart. (b) Authorized removals. Beer may be removed from a pilot brewing plant only for...

  19. 18 CFR 284.271 - Waiver.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... ENERGY OTHER REGULATIONS UNDER THE NATURAL GAS POLICY ACT OF 1978 AND RELATED AUTHORITIES CERTAIN SALES AND TRANSPORTATION OF NATURAL GAS UNDER THE NATURAL GAS POLICY ACT OF 1978 AND RELATED AUTHORITIES Emergency Natural Gas Sale, Transportation, and Exchange Transactions § 284.271 Waiver. The Commission may...

  20. 18 CFR 284.271 - Waiver.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... ENERGY OTHER REGULATIONS UNDER THE NATURAL GAS POLICY ACT OF 1978 AND RELATED AUTHORITIES CERTAIN SALES AND TRANSPORTATION OF NATURAL GAS UNDER THE NATURAL GAS POLICY ACT OF 1978 AND RELATED AUTHORITIES Emergency Natural Gas Sale, Transportation, and Exchange Transactions § 284.271 Waiver. The Commission may...

  1. 49 CFR 192.271 - Scope.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... BY PIPELINE: MINIMUM FEDERAL SAFETY STANDARDS Joining of Materials Other Than by Welding § 192.271 Scope. (a) This subpart prescribes minimum requirements for joining materials in pipelines, other than by welding. (b) This subpart does not apply to joining during the manufacture of pipe or...

  2. 49 CFR 192.271 - Scope.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... BY PIPELINE: MINIMUM FEDERAL SAFETY STANDARDS Joining of Materials Other Than by Welding § 192.271 Scope. (a) This subpart prescribes minimum requirements for joining materials in pipelines, other than by welding. (b) This subpart does not apply to joining during the manufacture of pipe or...

  3. 49 CFR 192.271 - Scope.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... BY PIPELINE: MINIMUM FEDERAL SAFETY STANDARDS Joining of Materials Other Than by Welding § 192.271 Scope. (a) This subpart prescribes minimum requirements for joining materials in pipelines, other than by welding. (b) This subpart does not apply to joining during the manufacture of pipe or...

  4. 49 CFR 192.271 - Scope.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... BY PIPELINE: MINIMUM FEDERAL SAFETY STANDARDS Joining of Materials Other Than by Welding § 192.271 Scope. (a) This subpart prescribes minimum requirements for joining materials in pipelines, other than by welding. (b) This subpart does not apply to joining during the manufacture of pipe or...

  5. 49 CFR 192.271 - Scope.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... BY PIPELINE: MINIMUM FEDERAL SAFETY STANDARDS Joining of Materials Other Than by Welding § 192.271 Scope. (a) This subpart prescribes minimum requirements for joining materials in pipelines, other than by welding. (b) This subpart does not apply to joining during the manufacture of pipe or...

  6. 7 CFR 271.6 - Complaint procedure.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM GENERAL INFORMATION AND DEFINITIONS § 271.6 Complaint... its choosing for handling program complaints filed by participants, potential participants, or...

  7. 7 CFR 271.6 - Complaint procedure.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM GENERAL INFORMATION AND DEFINITIONS § 271.6 Complaint... its choosing for handling program complaints filed by participants, potential participants, or...

  8. 7 CFR 271.6 - Complaint procedure.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM GENERAL INFORMATION AND DEFINITIONS § 271.6 Complaint... its choosing for handling program complaints filed by participants, potential participants, or...

  9. 47 CFR 0.271 - Authority delegated.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... of Strategic Planning and Policy Analysis § 0.271 Authority delegated. (a) Insofar as authority is... status, the Chief, Office of Strategic Planning and Policy Analysis is delegated authority to deny... Strategic Planning and Policy Analysis has primary authority. (b) Chief, Media Bureau...

  10. 14 CFR 271.2 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.2 Definitions... compensation under Subchapter II of Chapter 417 of the Statute. Essential air service is that air transportation which the Department has found to be essential under Subchapter II of Chapter 417 of the Statute. ...

  11. 31 CFR 27.1 - Purpose.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... DEPARTMENT OF THE TREASURY NAMES, SYMBOLS, ETC. § 27.1 Purpose. (a) The regulations in this part implement... penalty against any person who has misused the words, titles, abbreviations, initials, symbols, emblems... manufacture, produce, sell, possess, or use the words, titles, abbreviations, initials, symbols, emblems...

  12. 31 CFR 27.1 - Purpose.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... DEPARTMENT OF THE TREASURY NAMES, SYMBOLS, ETC. § 27.1 Purpose. (a) The regulations in this part implement... penalty against any person who has misused the words, titles, abbreviations, initials, symbols, emblems... manufacture, produce, sell, possess, or use the words, titles, abbreviations, initials, symbols, emblems...

  13. 36 CFR 271.4 - Commercial license.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... concerning the prevention of forest fires. (2) That the proposed use is consistent with the status of Smokey Bear as the symbol of forest fire prevention and does not in any way detract from such status. (3) That... 36 Parks, Forests, and Public Property 2 2012-07-01 2012-07-01 false Commercial license. 271.4...

  14. 36 CFR 271.4 - Commercial license.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... concerning the prevention of forest fires. (2) That the proposed use is consistent with the status of Smokey Bear as the symbol of forest fire prevention and does not in any way detract from such status. (3) That... 36 Parks, Forests, and Public Property 2 2014-07-01 2014-07-01 false Commercial license. 271.4...

  15. 36 CFR 271.4 - Commercial license.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... concerning the prevention of forest fires. (2) That the proposed use is consistent with the status of Smokey Bear as the symbol of forest fire prevention and does not in any way detract from such status. (3) That... 36 Parks, Forests, and Public Property 2 2013-07-01 2013-07-01 false Commercial license. 271.4...

  16. 36 CFR 27.1 - General objectives.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... COD NATIONAL SEASHORE; ZONING STANDARDS § 27.1 General objectives. (a) Consistent with the objectives... Cape Cod National Seashore will be conducted in a manner which will assure the widest possible public... Cape Cod National Seashore and situated in the towns of Provincetown, Truro, Wellfleet,...

  17. 48 CFR 719.271-1 - General.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-1 General. The purpose of this section is to prescribe responsibilities and procedures for carrying out the small business program policy set forth in 219.270, and in... disadvantaged business enterprises are included in the term “small business” when used in this subpart;...

  18. 25. BUILDING NO. 271, FUZE ASSEMBLY BUILDING, LOOKING SOUTHWEST AT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    25. BUILDING NO. 271, FUZE ASSEMBLY BUILDING, LOOKING SOUTHWEST AT SOUTHEAST AND NORTHEAST ELEVATIONS. BUILDING NO. 271-L, FIELD OFFICE, VISIBLE ON RIGHT. - Picatinny Arsenal, 200 Area, Shell Component Loading, State Route 15 near I-80, Dover, Morris County, NJ

  19. 40 CFR 271.15 - Requirements for compliance evaluation programs.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Final Authorization § 271.15 Requirements for compliance evaluation programs. (a) State programs shall... 40 Protection of Environment 27 2014-07-01 2014-07-01 false Requirements for compliance evaluation programs. 271.15 Section 271.15 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED)...

  20. 40 CFR 271.15 - Requirements for compliance evaluation programs.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Final Authorization § 271.15 Requirements for compliance evaluation programs. (a) State programs shall... 40 Protection of Environment 28 2013-07-01 2013-07-01 false Requirements for compliance evaluation programs. 271.15 Section 271.15 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED)...

  1. 40 CFR 271.15 - Requirements for compliance evaluation programs.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Final Authorization § 271.15 Requirements for compliance evaluation programs. (a) State programs shall... 40 Protection of Environment 28 2012-07-01 2012-07-01 false Requirements for compliance evaluation programs. 271.15 Section 271.15 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED)...

  2. 40 CFR 271.15 - Requirements for compliance evaluation programs.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Final Authorization § 271.15 Requirements for compliance evaluation programs. (a) State programs shall... 40 Protection of Environment 27 2011-07-01 2011-07-01 false Requirements for compliance evaluation programs. 271.15 Section 271.15 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED)...

  3. 7 CFR 27.1 - Meaning of words.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Meaning of words. 27.1 Section 27.1 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... CLASSIFICATION UNDER COTTON FUTURES LEGISLATION Regulations Definitions § 27.1 Meaning of words. Words used in...

  4. 40 CFR 271.5 - Elements of a program submission.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 28 2012-07-01 2012-07-01 false Elements of a program submission. 271.5 Section 271.5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Authorization § 271.5 Elements of a program submission. (a) Any State that seeks to administer a program under...

  5. 40 CFR 271.5 - Elements of a program submission.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 28 2013-07-01 2013-07-01 false Elements of a program submission. 271.5 Section 271.5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Authorization § 271.5 Elements of a program submission. (a) Any State that seeks to administer a program under...

  6. 40 CFR 271.5 - Elements of a program submission.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 27 2011-07-01 2011-07-01 false Elements of a program submission. 271.5 Section 271.5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Authorization § 271.5 Elements of a program submission. (a) Any State that seeks to administer a program under...

  7. 40 CFR 271.5 - Elements of a program submission.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 27 2014-07-01 2014-07-01 false Elements of a program submission. 271.5 Section 271.5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... Authorization § 271.5 Elements of a program submission. (a) Any State that seeks to administer a program under...

  8. 14 CFR 271.7 - Subsidy payout formula.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Subsidy payout formula. 271.7 Section 271.7 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.7 Subsidy...

  9. 36 CFR 271.7 - Power to revoke.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 36 Parks, Forests, and Public Property 2 2011-07-01 2011-07-01 false Power to revoke. 271.7 Section 271.7 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE USE OF âSMOKEY BEARâ SYMBOL § 271.7 Power to revoke. It is the intention of the regulations in this part that the Chief...

  10. 36 CFR 271.6 - Review of licenses.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Review of licenses. 271.6 Section 271.6 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE USE OF âSMOKEY BEARâ SYMBOL § 271.6 Review of licenses. The Chief will cooperate with the Association of State...

  11. 36 CFR 271.3 - Public service use.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 36 Parks, Forests, and Public Property 2 2011-07-01 2011-07-01 false Public service use. 271.3 Section 271.3 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE USE OF âSMOKEY BEARâ SYMBOL § 271.3 Public service use. The Chief may authorize the use of Smokey Bear for non...

  12. 36 CFR 271.2 - Use of official campaign materials.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 36 Parks, Forests, and Public Property 2 2011-07-01 2011-07-01 false Use of official campaign materials. 271.2 Section 271.2 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE USE OF âSMOKEY BEARâ SYMBOL § 271.2 Use of official campaign materials. Official Cooperative Forest...

  13. 36 CFR 271.6 - Review of licenses.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 36 Parks, Forests, and Public Property 2 2011-07-01 2011-07-01 false Review of licenses. 271.6 Section 271.6 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE USE OF âSMOKEY BEARâ SYMBOL § 271.6 Review of licenses. The Chief will cooperate with the Association of State...

  14. 14 CFR 271.3 - Carrier subsidy need.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Carrier subsidy need. 271.3 Section 271.3 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC REGULATIONS GUIDELINES FOR SUBSIDIZING AIR CARRIERS PROVIDING ESSENTIAL AIR TRANSPORTATION § 271.3...

  15. 7 CFR 27.1 - Meaning of words.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 2 2012-01-01 2012-01-01 false Meaning of words. 27.1 Section 27.1 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... CLASSIFICATION UNDER COTTON FUTURES LEGISLATION Regulations Definitions § 27.1 Meaning of words. Words used...

  16. 7 CFR 27.1 - Meaning of words.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 2 2014-01-01 2014-01-01 false Meaning of words. 27.1 Section 27.1 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... CLASSIFICATION UNDER COTTON FUTURES LEGISLATION Regulations Definitions § 27.1 Meaning of words. Words used...

  17. 7 CFR 27.1 - Meaning of words.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 2 2011-01-01 2011-01-01 false Meaning of words. 27.1 Section 27.1 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... CLASSIFICATION UNDER COTTON FUTURES LEGISLATION Regulations Definitions § 27.1 Meaning of words. Words used...

  18. 7 CFR 27.1 - Meaning of words.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 2 2013-01-01 2013-01-01 false Meaning of words. 27.1 Section 27.1 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... CLASSIFICATION UNDER COTTON FUTURES LEGISLATION Regulations Definitions § 27.1 Meaning of words. Words used...

  19. 40 CFR 271.19 - EPA review of State permits.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 28 2013-07-01 2013-07-01 false EPA review of State permits. 271.19... Authorization § 271.19 EPA review of State permits. (a) The Regional Administrator may comment on permit applications and draft permits as provided in the Memorandum of Agreement under § 271.8. (b) Where...

  20. 40 CFR 271.19 - EPA review of State permits.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 27 2011-07-01 2011-07-01 false EPA review of State permits. 271.19... Authorization § 271.19 EPA review of State permits. (a) The Regional Administrator may comment on permit applications and draft permits as provided in the Memorandum of Agreement under § 271.8. (b) Where...

  1. 40 CFR 271.19 - EPA review of State permits.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 27 2014-07-01 2014-07-01 false EPA review of State permits. 271.19... Authorization § 271.19 EPA review of State permits. (a) The Regional Administrator may comment on permit applications and draft permits as provided in the Memorandum of Agreement under § 271.8. (b) Where...

  2. 12 CFR 271.7 - Exemptions from disclosure.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Exemptions from disclosure. 271.7 Section 271.7... AVAILABILITY OF INFORMATION § 271.7 Exemptions from disclosure. (a) Types of records exempt from disclosure. Pursuant to 5 U.S.C. 552(b), the following records of the Committee are exempt from disclosure under this...

  3. 37 CFR 2.71 - Amendments to correct informalities.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Amendments to correct informalities. 2.71 Section 2.71 Patents, Trademarks, and Copyrights UNITED STATES PATENT AND TRADEMARK OFFICE, DEPARTMENT OF COMMERCE RULES OF PRACTICE IN TRADEMARK CASES Amendment of Application § 2.71 Amendments to...

  4. 37 CFR 2.71 - Amendments to correct informalities.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2011-07-01 2011-07-01 false Amendments to correct informalities. 2.71 Section 2.71 Patents, Trademarks, and Copyrights UNITED STATES PATENT AND TRADEMARK OFFICE, DEPARTMENT OF COMMERCE RULES OF PRACTICE IN TRADEMARK CASES Amendment of Application § 2.71 Amendments to...

  5. 48 CFR 719.271 - Agency program direction and operation.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Agency program direction and operation. 719.271 Section 719.271 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271 Agency program direction...

  6. 48 CFR 853.271-2 - Education Programs.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Education Programs. 853.271-2 Section 853.271-2 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS CLAUSES AND FORMS FORMS Prescription of Forms 853.271-2 Education Programs. To obtain education or...

  7. 48 CFR 853.271-2 - Education Programs.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Education Programs. 853.271-2 Section 853.271-2 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS CLAUSES AND FORMS FORMS Prescription of Forms 853.271-2 Education Programs. To obtain education or...

  8. 48 CFR 853.271-2 - Education Programs.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Education Programs. 853.271-2 Section 853.271-2 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS CLAUSES AND FORMS FORMS Prescription of Forms 853.271-2 Education Programs. To obtain education or...

  9. 48 CFR 853.271-2 - Education Programs.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Education Programs. 853.271-2 Section 853.271-2 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS CLAUSES AND FORMS FORMS Prescription of Forms 853.271-2 Education Programs. To obtain education...

  10. 48 CFR 853.271-2 - Education Programs.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Education Programs. 853.271-2 Section 853.271-2 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS CLAUSES AND FORMS FORMS Prescription of Forms 853.271-2 Education Programs. To obtain education...

  11. CD271 is an imperfect marker for melanoma initiating cells

    PubMed Central

    Cheli, Yann; Bonnazi, Vanessa F.; Jacquel, Arnaud; Allegra, Maryline; Donatis, Gian Marco De; Bahadoran, Philippe; Bertolotto, Corine; Ballotti, Robert

    2014-01-01

    Understanding the molecular and cellular processes underlying melanoma plasticity and heterogeneity is of paramount importance to improve the efficiency of current treatment and to overcome resistance to chemotherapy drugs. The notion of plasticity and heterogeneity implies the existence of melanoma cell populations with different phenotypic and tumorigenic properties. Using melanoma cell lines and melanoma cells freshly isolated from patient biopsies, we investigated the relationship between ABCB5+, CD271+ and low-MITF, expressing populations that were reported to display melanoma initiating cell properties. Here, we showed that ABCB5+ and CD271+ populations poorly overlap. However, we found that the CD271+ population is enriched in low-MITF cells and expresses a higher level of stemness genes, such as OCT4, NANOG and NES. These features could explain the increased tumorigenicity of the CD271+ cells. The rapid conversion of CD271+ to CD271− cells in vitro demonstrates the plasticity ability of melanoma cells. Finally, we observed that the transient slow-growing population contains only CD271+ cells that are highly tumorigenic. However, the fast growing/CD271+ population exhibits a poor tumorigenic ability. Taking together, our data show that CD271 is an imperfect marker for melanoma initiating cells, but may be useful to identify melanoma cells with an increased stemness and tumorigenic potential. PMID:25105565

  12. CD271 is an imperfect marker for melanoma initiating cells.

    PubMed

    Cheli, Yann; Bonnazi, Vanessa F; Jacquel, Arnaud; Allegra, Maryline; De Donatis, Gian Marco; Bahadoran, Philippe; Bertolotto, Corine; Ballotti, Robert

    2014-07-30

    Understanding the molecular and cellular processes underlying melanoma plasticity and heterogeneity is of paramount importance to improve the efficiency of current treatment and to overcome resistance to chemotherapy drugs. The notion of plasticity and heterogeneity implies the existence of melanoma cell populations with different phenotypic and tumorigenic properties. Using melanoma cell lines and melanoma cells freshly isolated from patient biopsies, we investigated the relationship between ABCB5+, CD271+ and low-MITF, expressing populations that were reported to display melanoma initiating cell properties. Here, we showed that ABCB5+ and CD271+ populations poorly overlap. However, we found that the CD271+ population is enriched in low-MITF cells and expresses a higher level of stemness genes, such as OCT4, NANOG and NES. These features could explain the increased tumorigenicity of the CD271+ cells. The rapid conversion of CD271+ to CD271- cells in vitro demonstrates the plasticity ability of melanoma cells. Finally, we observed that the transient slow-growing population contains only CD271+ cells that are highly tumorigenic. However, the fast growing/CD271+ population exhibits a poor tumorigenic ability. Taking together, our data show that CD271 is an imperfect marker for melanoma initiating cells, but may be useful to identify melanoma cells with an increased stemness and tumorigenic potential.

  13. 48 CFR 642.271 - Government Technical Monitor (GTM).

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... MANAGEMENT CONTRACT ADMINISTRATION AND AUDIT SERVICES Contract Administration Services 642.271 Government..., or because of special skills or knowledge necessary for monitoring the contractor's work....

  14. 40 CFR 271.5 - Elements of a program submission.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 26 2010-07-01 2010-07-01 false Elements of a program submission. 271.5 Section 271.5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final...

  15. 40 CFR 271.7 - Attorney General's statement.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 26 2010-07-01 2010-07-01 false Attorney General's statement. 271.7 Section 271.7 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final...

  16. 40 CFR 271.17 - Sharing of information.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 271.17 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.17... State regarding facilities and sites for the treatment, storage, and disposal of hazardous waste. Such...

  17. 40 CFR 271.26 - Requirements for used oil management.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.26 Requirements for used oil management. The State shall have standards for... to EPA under § 271.5) EPA to allow the use of used oil (that is not mixed with hazardous waste and...

  18. 40 CFR 271.14 - Requirements for permitting.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 26 2010-07-01 2010-07-01 false Requirements for permitting. 271.14 Section 271.14 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final...

  19. 28 CFR 2.71 - Application for parole.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 28 Judicial Administration 1 2013-07-01 2013-07-01 false Application for parole. 2.71 Section 2.71 Judicial Administration DEPARTMENT OF JUSTICE PAROLE, RELEASE, SUPERVISION AND RECOMMITMENT OF PRISONERS... Application for parole. (a) A prisoner (including a committed youth offender) desiring to apply for...

  20. 28 CFR 2.71 - Application for parole.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 28 Judicial Administration 1 2011-07-01 2011-07-01 false Application for parole. 2.71 Section 2.71 Judicial Administration DEPARTMENT OF JUSTICE PAROLE, RELEASE, SUPERVISION AND RECOMMITMENT OF PRISONERS... Application for parole. (a) A prisoner (including a committed youth offender) desiring to apply for...

  1. 28 CFR 2.71 - Application for parole.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 28 Judicial Administration 1 2014-07-01 2014-07-01 false Application for parole. 2.71 Section 2.71 Judicial Administration DEPARTMENT OF JUSTICE PAROLE, RELEASE, SUPERVISION AND RECOMMITMENT OF PRISONERS... Application for parole. (a) A prisoner (including a committed youth offender) desiring to apply for...

  2. 28 CFR 2.71 - Application for parole.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 28 Judicial Administration 1 2012-07-01 2012-07-01 false Application for parole. 2.71 Section 2.71 Judicial Administration DEPARTMENT OF JUSTICE PAROLE, RELEASE, SUPERVISION AND RECOMMITMENT OF PRISONERS... Application for parole. (a) A prisoner (including a committed youth offender) desiring to apply for...

  3. 27 CFR 41.271 - Power of attorney.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2012-04-01 2011-04-01 true Power of attorney. 41.271 Section 41.271 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY (CONTINUED) TOBACCO IMPORTATION OF TOBACCO PRODUCTS, CIGARETTE PAPERS AND TUBES, AND...

  4. 27 CFR 41.271 - Power of attorney.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2014-04-01 2014-04-01 false Power of attorney. 41.271 Section 41.271 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY (CONTINUED) TOBACCO IMPORTATION OF TOBACCO PRODUCTS, CIGARETTE PAPERS AND TUBES, AND...

  5. 27 CFR 41.271 - Power of attorney.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Power of attorney. 41.271 Section 41.271 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY (CONTINUED) TOBACCO IMPORTATION OF TOBACCO PRODUCTS, CIGARETTE PAPERS AND TUBES, AND...

  6. 27 CFR 41.271 - Power of attorney.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2013-04-01 2013-04-01 false Power of attorney. 41.271 Section 41.271 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY (CONTINUED) TOBACCO IMPORTATION OF TOBACCO PRODUCTS, CIGARETTE PAPERS AND TUBES, AND...

  7. 12 CFR 271.9 - Fee schedules; waiver of fees.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 271.9 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) FEDERAL OPEN MARKET COMMITTEE RULES... pursuant to § 271.5 will pay the applicable fees, unless the request includes a limitation on fees to be... because it is likely to contribute significantly to public understanding of the operation or activities...

  8. 40 CFR 415.271 - Specialized definitions. [Reserved

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 30 2013-07-01 2012-07-01 true Specialized definitions. 415.271 Section 415.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS INORGANIC CHEMICALS MANUFACTURING POINT SOURCE CATEGORY Borax Production Subcategory...

  9. 40 CFR 415.271 - Specialized definitions. [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 29 2014-07-01 2012-07-01 true Specialized definitions. 415.271 Section 415.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS INORGANIC CHEMICALS MANUFACTURING POINT SOURCE CATEGORY Borax Production Subcategory...

  10. 40 CFR 415.271 - Specialized definitions. [Reserved

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 30 2012-07-01 2012-07-01 false Specialized definitions. 415.271 Section 415.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS INORGANIC CHEMICALS MANUFACTURING POINT SOURCE CATEGORY Borax Production Subcategory...

  11. 27 CFR 19.271 - Construction of buildings

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Construction of buildings 19.271 Section 19.271 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU... Construction of buildings Buildings in which spirits, denatured spirits, articles, or wines are...

  12. 50 CFR 216.271 - Effective dates and definitions.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 9 2011-10-01 2011-10-01 false Effective dates and definitions. 216.271 Section 216.271 Wildlife and Fisheries NATIONAL MARINE FISHERIES SERVICE, NATIONAL OCEANIC AND ATMOSPHERIC... February 4, 2011, through January 14, 2014. (b) The following definitions are utilized in these regulations...

  13. 7 CFR 205.271 - Facility pest management practice standard.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 3 2012-01-01 2012-01-01 false Facility pest management practice standard. 205.271... Requirements § 205.271 Facility pest management practice standard. (a) The producer or handler of an organic facility must use management practices to prevent pests, including but not limited to: (1) Removal of pest...

  14. 7 CFR 205.271 - Facility pest management practice standard.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 3 2014-01-01 2014-01-01 false Facility pest management practice standard. 205.271... Requirements § 205.271 Facility pest management practice standard. (a) The producer or handler of an organic facility must use management practices to prevent pests, including but not limited to: (1) Removal of pest...

  15. 48 CFR 1352.271-87 - Changes-ship repair.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Changes-ship repair. 1352.271-87 Section 1352.271-87 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND...—ship repair. As prescribed in 48 CFR 1371.118, insert the following clause: Changes—Ship Repair (APR...

  16. 48 CFR 1352.271-87 - Changes-ship repair.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Changes-ship repair. 1352.271-87 Section 1352.271-87 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND...—ship repair. As prescribed in 48 CFR 1371.118, insert the following clause: Changes—Ship Repair (APR...

  17. 48 CFR 1352.271-87 - Changes-ship repair.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Changes-ship repair. 1352.271-87 Section 1352.271-87 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND...—ship repair. As prescribed in 48 CFR 1371.118, insert the following clause: Changes—Ship Repair (APR...

  18. 48 CFR 1352.271-87 - Changes-ship repair.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Changes-ship repair. 1352.271-87 Section 1352.271-87 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND...—ship repair. As prescribed in 48 CFR 1371.118, insert the following clause: Changes—Ship Repair (APR...

  19. 48 CFR 1352.271-87 - Changes-ship repair.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Changes-ship repair. 1352.271-87 Section 1352.271-87 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CLAUSES AND...—ship repair. As prescribed in 48 CFR 1371.118, insert the following clause: Changes—Ship Repair (APR...

  20. 12 CFR 27.1 - Scope and OMB control number.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 1 2010-01-01 2010-01-01 false Scope and OMB control number. 27.1 Section 27.1 Banks and Banking COMPTROLLER OF THE CURRENCY, DEPARTMENT OF THE TREASURY FAIR HOUSING HOME LOAN DATA... banks and their subsidiaries, which make home loans for the purpose of purchasing, construction...

  1. 48 CFR 1352.271-86 - Lay days.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Lay days. 1352.271-86... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-86 Lay days. As prescribed in 48 CFR 1371.117, insert the following clause: Lay Days (APR 2010) (a) A lay day is defined as...

  2. 48 CFR 1352.271-86 - Lay days.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Lay days. 1352.271-86... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-86 Lay days. As prescribed in 48 CFR 1371.117, insert the following clause: Lay Days (APR 2010) (a) A lay day is defined as...

  3. 48 CFR 1352.271-86 - Lay days.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Lay days. 1352.271-86... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-86 Lay days. As prescribed in 48 CFR 1371.117, insert the following clause: Lay Days (APR 2010) (a) A lay day is defined as...

  4. 40 CFR 271.7 - Attorney General's statement.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 28 2012-07-01 2012-07-01 false Attorney General's statement. 271.7... Authorization § 271.7 Attorney General's statement. (a) Any State that seeks to administer a program under this... pertaining to the State program. Note: EPA will supply States with an Attorney General's statement format on...

  5. 28 CFR 27.1 - Making a protected disclosure.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Section 27.1 Judicial Administration DEPARTMENT OF JUSTICE WHISTLEBLOWER PROTECTION FOR FEDERAL BUREAU OF INVESTIGATION EMPLOYEES Protected Disclosures of Information § 27.1 Making a protected disclosure. (a) When an employee of, or applicant for employment with, the Federal Bureau of Investigation (FBI) (FBI...

  6. 28 CFR 27.1 - Making a protected disclosure.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Section 27.1 Judicial Administration DEPARTMENT OF JUSTICE WHISTLEBLOWER PROTECTION FOR FEDERAL BUREAU OF INVESTIGATION EMPLOYEES Protected Disclosures of Information § 27.1 Making a protected disclosure. (a) When an employee of, or applicant for employment with, the Federal Bureau of Investigation (FBI) (FBI...

  7. 28 CFR 27.1 - Making a protected disclosure.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Section 27.1 Judicial Administration DEPARTMENT OF JUSTICE WHISTLEBLOWER PROTECTION FOR FEDERAL BUREAU OF INVESTIGATION EMPLOYEES Protected Disclosures of Information § 27.1 Making a protected disclosure. (a) When an employee of, or applicant for employment with, the Federal Bureau of Investigation (FBI) (FBI...

  8. 7 CFR 271.3 - Delegations to FNS for administration.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 4 2010-01-01 2010-01-01 false Delegations to FNS for administration. 271.3 Section 271.3 Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE... administration and benefit delivery. FNS is authorized to initiate program research and evaluation efforts...

  9. 40 CFR 271.3 - Availability of final authorization.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ..., including electronic signature requirements, and imposed under the authority of the Hazardous Waste.... 271.3 Section 271.3 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final...

  10. 48 CFR 1352.271-86 - Lay days.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Lay days. 1352.271-86... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-86 Lay days. As prescribed in 48 CFR 1371.117, insert the following clause: Lay Days (APR 2010) (a) A lay day is defined as...

  11. 36 CFR 271.3 - Public service use.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Public service use. 271.3 Section 271.3 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE USE OF âSMOKEY..., in his judgment, contribute to public information and education concerning the prevention of...

  12. 36 CFR 271.2 - Use of official campaign materials.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... of increasing public information regarding forest fire prevention. ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Use of official campaign materials. 271.2 Section 271.2 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF...

  13. 36 CFR 271.2 - Use of official campaign materials.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... of increasing public information regarding forest fire prevention. ... 36 Parks, Forests, and Public Property 2 2013-07-01 2013-07-01 false Use of official campaign materials. 271.2 Section 271.2 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE...

  14. 36 CFR 271.3 - Public service use.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 36 Parks, Forests, and Public Property 2 2013-07-01 2013-07-01 false Public service use. 271.3 Section 271.3 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE USE OF âSMOKEY..., in his judgment, contribute to public information and education concerning the prevention of forest...

  15. 36 CFR 271.2 - Use of official campaign materials.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... of increasing public information regarding forest fire prevention. ... 36 Parks, Forests, and Public Property 2 2012-07-01 2012-07-01 false Use of official campaign materials. 271.2 Section 271.2 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE...

  16. 36 CFR 271.3 - Public service use.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 36 Parks, Forests, and Public Property 2 2014-07-01 2014-07-01 false Public service use. 271.3 Section 271.3 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE USE OF âSMOKEY..., in his judgment, contribute to public information and education concerning the prevention of forest...

  17. 36 CFR 271.2 - Use of official campaign materials.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... of increasing public information regarding forest fire prevention. ... 36 Parks, Forests, and Public Property 2 2014-07-01 2014-07-01 false Use of official campaign materials. 271.2 Section 271.2 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE...

  18. 36 CFR 271.3 - Public service use.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 36 Parks, Forests, and Public Property 2 2012-07-01 2012-07-01 false Public service use. 271.3 Section 271.3 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE USE OF âSMOKEY..., in his judgment, contribute to public information and education concerning the prevention of forest...

  19. 40 CFR 271.3 - Availability of final authorization.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 28 2012-07-01 2012-07-01 false Availability of final authorization. 271.3 Section 271.3 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... basis of Federal self-implementing statutory provisions that were in effect 12 months prior to the...

  20. 48 CFR 719.271-6 - Small business screening procedure.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Small business screening procedure. 719.271-6 Section 719.271-6 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL... host country) with educational or nonprofit institutions; or collaborative assistance...

  1. 28 CFR 27.1 - Making a protected disclosure.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 28 Judicial Administration 1 2013-07-01 2013-07-01 false Making a protected disclosure. 27.1... INVESTIGATION EMPLOYEES Protected Disclosures of Information § 27.1 Making a protected disclosure. (a) When an... Responsibility (OPR), the Department's Office of Inspector General (OIG), the FBI Office of...

  2. 48 CFR 1816.405-271 - Base fee.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 6 2012-10-01 2012-10-01 false Base fee. 1816.405-271... CONTRACTING METHODS AND CONTRACT TYPES TYPES OF CONTRACTS Incentive Contracts 1816.405-271 Base fee. (a) A base fee shall not be used on CPAF contracts for which the periodic award fee evaluations are...

  3. 48 CFR 1816.405-271 - Base fee.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 6 2013-10-01 2013-10-01 false Base fee. 1816.405-271... CONTRACTING METHODS AND CONTRACT TYPES TYPES OF CONTRACTS Incentive Contracts 1816.405-271 Base fee. (a) A base fee shall not be used on CPAF contracts for which the periodic award fee evaluations are...

  4. 48 CFR 1816.405-271 - Base fee.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Base fee. 1816.405-271... CONTRACTING METHODS AND CONTRACT TYPES TYPES OF CONTRACTS Incentive Contracts 1816.405-271 Base fee. (a) A base fee shall not be used on CPAF contracts for which the periodic award fee evaluations are...

  5. 48 CFR 1816.405-271 - Base fee.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 6 2014-10-01 2014-10-01 false Base fee. 1816.405-271... CONTRACTING METHODS AND CONTRACT TYPES TYPES OF CONTRACTS Incentive Contracts 1816.405-271 Base fee. (a) A base fee shall not be used on CPAF contracts for which the periodic award fee evaluations are...

  6. 48 CFR 1816.405-271 - Base fee.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 6 2011-10-01 2011-10-01 false Base fee. 1816.405-271... CONTRACTING METHODS AND CONTRACT TYPES TYPES OF CONTRACTS Incentive Contracts 1816.405-271 Base fee. (a) A base fee shall not be used on CPAF contracts for which the periodic award fee evaluations are...

  7. 48 CFR 852.271-75 - Extension of contract period.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Extension of contract period. 852.271-75 Section 852.271-75 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Texts of Provisions and Clauses...

  8. 14 CFR 271.3 - Carrier subsidy need.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Carrier subsidy need. 271.3 Section 271.3 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC... subsidy need. In establishing the subsidy for an air carrier providing essential air service at an...

  9. 14 CFR 271.3 - Carrier subsidy need.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 4 2012-01-01 2012-01-01 false Carrier subsidy need. 271.3 Section 271.3 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC... subsidy need. In establishing the subsidy for an air carrier providing essential air service at an...

  10. 14 CFR 271.3 - Carrier subsidy need.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 4 2014-01-01 2014-01-01 false Carrier subsidy need. 271.3 Section 271.3 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC... subsidy need. In establishing the subsidy for an air carrier providing essential air service at an...

  11. 14 CFR 271.3 - Carrier subsidy need.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false Carrier subsidy need. 271.3 Section 271.3 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC... subsidy need. In establishing the subsidy for an air carrier providing essential air service at an...

  12. 48 CFR 3437.271 - Services of consultants clause.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 7 2010-10-01 2010-10-01 false Services of consultants clause. 3437.271 Section 3437.271 Federal Acquisition Regulations System DEPARTMENT OF EDUCATION ACQUISITION REGULATION SPECIAL CATEGORIES OF CONTRACTING SERVICE CONTRACTING Consulting Services...

  13. 48 CFR 719.271-5 - Cognizant technical officers.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Cognizant technical officers. 719.271-5 Section 719.271-5 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL... contractual implementation: (a) Consult with SDB on the availability and capabilities of small business...

  14. 40 CFR 415.271 - Specialized definitions. [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 28 2010-07-01 2010-07-01 true Specialized definitions. 415.271 Section 415.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS INORGANIC CHEMICALS MANUFACTURING POINT SOURCE CATEGORY Borax Production Subcategory...

  15. 40 CFR 415.271 - Specialized definitions. [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 29 2011-07-01 2009-07-01 true Specialized definitions. 415.271 Section 415.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS INORGANIC CHEMICALS MANUFACTURING POINT SOURCE CATEGORY Borax Production Subcategory...

  16. 48 CFR 1352.271-86 - Lay days.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... associated with lay days except for costs directly related to the changed work. (End of clause) ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Lay days. 1352.271-86... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1352.271-86 Lay days. As prescribed...

  17. 28 CFR 2.71 - Application for parole.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 1 2010-07-01 2010-07-01 false Application for parole. 2.71 Section 2.71 Judicial Administration DEPARTMENT OF JUSTICE PAROLE, RELEASE, SUPERVISION AND RECOMMITMENT OF PRISONERS, YOUTH OFFENDERS, AND JUVENILE DELINQUENTS District of Columbia Code: Prisoners and Parolees §...

  18. 40 CFR 271.16 - Requirements for enforcement authority.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    .... 271.16 Section 271.16 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID... adverse effects upon human health and the environment resulting from the unauthorized activity, whether or... or the environment. (e) Any State authority used to issue an enforceable document either in lieu of...

  19. CD271 determines migratory properties of melanoma cells.

    PubMed

    Radke, Josefine; Roßner, Florian; Redmer, Torben

    2017-08-29

    Melanoma cell expression of the nerve growth factor receptor CD271 is associated with stem-like properties. However, the contributing role of the receptor in melanoma cell migration is elusive. Here, we explored extracranial (skin, soft tissue, lymph node and liver, n = 13) and matched brain metastases (BM, n = 12) and observed a heterogeneous distribution of phenotypically distinct subsets of CD271(+) cells. In addition, we observed that CD271 expression gradually rises along with melanoma progression and metastasis by exploration of publicly available expression data of nevi, primary melanoma (n = 31) and melanoma metastases (n = 54). Furthermore, we observed highest levels of CD271 in BM. Sub-clustering identified 99 genes differentially expressed among CD271(high) and CD271(low) (p < 0.05) BM-subgroups. Comparative analysis of subsets revealed increased ( ≥ 1.5fold, log2) expression of migration-associated genes and enrichment of CD271-responsible genes involved in DNA-repair and stemness. Live cell-imaging based scratch-wound assays of melanoma cells with stable knock-down of CD271 revealed a significantly reduced cell migration (3.9fold, p = 1.2E-04) and a reduced expression of FGF13, CSPG4, HMGA2 and AKT3 major candidate regulatory genes of melanoma cell migration. In summary, we provide new insights in melanoma cell migration and suggest that CD271 serves as a candidate regulator, sufficient to determine cellular properties of melanoma brain metastatic cells.

  20. 27 CFR 46.271 - Entry, examination and testimony.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... PRODUCTS AND CIGARETTE PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette Tubes Held for Sale on April 1, 2009 Ttb Authorities § 46.271 Entry, examination and testimony...

  1. 27 CFR 46.271 - Entry, examination and testimony.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... PRODUCTS AND CIGARETTE PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette Tubes Held for Sale on April 1, 2009 Ttb Authorities § 46.271 Entry, examination and...

  2. 27 CFR 46.271 - Entry, examination and testimony.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... PRODUCTS AND CIGARETTE PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette Tubes Held for Sale on April 1, 2009 Ttb Authorities § 46.271 Entry, examination and...

  3. 27 CFR 46.271 - Entry, examination and testimony.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... PRODUCTS AND CIGARETTE PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette Tubes Held for Sale on April 1, 2009 Ttb Authorities § 46.271 Entry, examination and...

  4. Interior view showing interface between building 271 and with building ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior view showing interface between building 271 and with building 87 in background; camera facing west. - Mare Island Naval Shipyard, Mechanics Shop, Waterfront Avenue, west side between A Street & Third Street, Vallejo, Solano County, CA

  5. 47 CFR 27.1 - Basis and purpose.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES MISCELLANEOUS WIRELESS COMMUNICATIONS SERVICES General Information § 27.1 Basis and purpose. This section contains the statutory basis... rules for miscellaneous wireless communications services (WCS) in this part are promulgated under...

  6. 47 CFR 27.1 - Basis and purpose.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES MISCELLANEOUS WIRELESS COMMUNICATIONS SERVICES General Information § 27.1 Basis and purpose. This section contains the statutory basis... rules for miscellaneous wireless communications services (WCS) in this part are promulgated under...

  7. 47 CFR 27.1 - Basis and purpose.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES MISCELLANEOUS WIRELESS COMMUNICATIONS SERVICES General Information § 27.1 Basis and purpose. This section contains the statutory basis... rules for miscellaneous wireless communications services (WCS) in this part are promulgated under...

  8. 47 CFR 27.1 - Basis and purpose.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES MISCELLANEOUS WIRELESS COMMUNICATIONS SERVICES General Information § 27.1 Basis and purpose. This section contains the statutory basis... rules for miscellaneous wireless communications services (WCS) in this part are promulgated under...

  9. EPA Survey Shows $271 Billion Needed for Nations Wastewater Infrastructure

    EPA Pesticide Factsheets

    WASHINGTON - The U.S. Environmental Protection Agency (EPA) today released a survey showing that $271 billion is needed to maintain and improve the nation's wastewater infrastructure, including the pipes that carry wastewater to treatment plants, th

  10. 50 CFR 216.271 - Effective dates and definitions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... cetacean species (not including mother/calf pairs, unless of species of concern listed in § 216.271(b)(1... whales, blue whales, fin whales, or sei whales. (iii) A group of 2 or more cetaceans of any...

  11. CD271+ Osteosarcoma Cells Display Stem-Like Properties

    PubMed Central

    Tian, Jiguang; Li, Xin; Si, Meng; Liu, Ting; Li, Jianmin

    2014-01-01

    Cancer stem cell (CSC) theory has been proposed and verified in many cancers. The existence of osteosarcoma CSCs has been confirmed for many years and multiple surface markers have been employed to identify them. In this study, we identified CD271+ subpopulation of osteosarcoma displaying stem-like properties. CD271, known as the neural crest nerve growth factor receptor, is the marker of bone marrow mesenchymal stem cells (MSCs) and human melanoma-initiating cells. We discovered that CD271 was expressed differentially in diverse types of human osteosarcoma and stabilized cell lines. CD271+ osteosarcoma cells displayed most of the properties of CSC, such as self-renewal, differentiation, drug resistance and tumorigenicity in vivo. Nanog, Oct3/4, STAT3, DNA-PKcs, Bcl-2 and ABCG2 were more expressed in CD271+ cells compared with CD271− cells. Our study supported the osteosarcoma CSC hypothesis and, to a certain extent, revealed one of the possible mechanisms involved in maintaining CSCs properties. PMID:24893164

  12. CD271+ osteosarcoma cells display stem-like properties.

    PubMed

    Tian, Jiguang; Li, Xin; Si, Meng; Liu, Ting; Li, Jianmin

    2014-01-01

    Cancer stem cell (CSC) theory has been proposed and verified in many cancers. The existence of osteosarcoma CSCs has been confirmed for many years and multiple surface markers have been employed to identify them. In this study, we identified CD271(+) subpopulation of osteosarcoma displaying stem-like properties. CD271, known as the neural crest nerve growth factor receptor, is the marker of bone marrow mesenchymal stem cells (MSCs) and human melanoma-initiating cells. We discovered that CD271 was expressed differentially in diverse types of human osteosarcoma and stabilized cell lines. CD271(+) osteosarcoma cells displayed most of the properties of CSC, such as self-renewal, differentiation, drug resistance and tumorigenicity in vivo. Nanog, Oct3/4, STAT3, DNA-PKcs, Bcl-2 and ABCG2 were more expressed in CD271(+) cells compared with CD271- cells. Our study supported the osteosarcoma CSC hypothesis and, to a certain extent, revealed one of the possible mechanisms involved in maintaining CSCs properties.

  13. Atomic Mass and Nuclear Binding Energy for Po-271 (Polonium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Po-271 (Polonium, atomic number Z = 84, mass number A = 271).

  14. Atomic Mass and Nuclear Binding Energy for At-271 (Astatine)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope At-271 (Astatine, atomic number Z = 85, mass number A = 271).

  15. Atomic Mass and Nuclear Binding Energy for Db-271 (Dubnium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Db-271 (Dubnium, atomic number Z = 105, mass number A = 271).

  16. Atomic Mass and Nuclear Binding Energy for Pa-271 (Protactinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Pa-271 (Protactinium, atomic number Z = 91, mass number A = 271).

  17. Atomic Mass and Nuclear Binding Energy for Fm-271 (Fermium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Fm-271 (Fermium, atomic number Z = 100, mass number A = 271).

  18. Atomic Mass and Nuclear Binding Energy for Mt-271 (Meitnerium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Mt-271 (Meitnerium, atomic number Z = 109, mass number A = 271).

  19. Atomic Mass and Nuclear Binding Energy for Ac-271 (Actinium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Ac-271 (Actinium, atomic number Z = 89, mass number A = 271).

  20. Atomic Mass and Nuclear Binding Energy for Lr-271 (Lawrencium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Lr-271 (Lawrencium, atomic number Z = 103, mass number A = 271).

  1. Atomic Mass and Nuclear Binding Energy for Sg-271 (Seaborgium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Sg-271 (Seaborgium, atomic number Z = 106, mass number A = 271).

  2. Atomic Mass and Nuclear Binding Energy for Bk-271 (Berkelium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Bk-271 (Berkelium, atomic number Z = 97, mass number A = 271).

  3. Atomic Mass and Nuclear Binding Energy for Bh-271 (Bohrium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Bh-271 (Bohrium, atomic number Z = 107, mass number A = 271).

  4. Atomic Mass and Nuclear Binding Energy for Md-271 (Mendelevium)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume B `Nuclei with Z = 55 - 100' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms', and additionally including data for nuclei with Z = 101 - 130. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Md-271 (Mendelevium, atomic number Z = 101, mass number A = 271).

  5. 40 CFR 271.19 - EPA review of State permits.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Section 271.19 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final... State program requirements, whether or not that condition was included in the final permit. (3) The...

  6. 7 CFR 205.271 - Facility pest management practice standard.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...) ORGANIC FOODS PRODUCTION ACT PROVISIONS NATIONAL ORGANIC PROGRAM Organic Production and Handling Requirements § 205.271 Facility pest management practice standard. (a) The producer or handler of an organic... habitat, food sources, and breeding areas; (2) Prevention of access to handling facilities; and (3...

  7. 48 CFR 1352.271-73 - Schedule of work.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Schedule of work. 1352.271... of work. As prescribed in 48 CFR 1371.104, insert the following clause. Schedule of Work (APR 2010... arrival at the contractor's facility: (1) Production Schedule. (2) Work Package Network. (3) Total...

  8. 48 CFR 1352.271-73 - Schedule of work.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Schedule of work. 1352.271... of work. As prescribed in 48 CFR 1371.104, insert the following clause. Schedule of Work (APR 2010... arrival at the contractor's facility: (1) Production Schedule. (2) Work Package Network. (3) Total...

  9. 48 CFR 1352.271-73 - Schedule of work.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Schedule of work. 1352.271... of work. As prescribed in 48 CFR 1371.104, insert the following clause. Schedule of Work (APR 2010... arrival at the contractor's facility: (1) Production Schedule. (2) Work Package Network. (3) Total...

  10. 40 CFR 97.271 - Initial certification and recertification procedures.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... monitoring system shall not be considered valid quality-assured data beginning with the date and hour of... (CONTINUED) AIR PROGRAMS (CONTINUED) FEDERAL NOX BUDGET TRADING PROGRAM AND CAIR NOX AND SO2 TRADING PROGRAMS Monitoring and Reporting § 97.271 Initial certification and recertification procedures. (a) The owner...

  11. 40 CFR 271.18 - Coordination with other programs.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 255) as responsible for the development and implementation of State solid waste management plans under....18 Section 271.18 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... program which may affect the planning for and development of hazardous waste management facilities...

  12. 40 CFR 271.18 - Coordination with other programs.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 255) as responsible for the development and implementation of State solid waste management plans under....18 Section 271.18 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... program which may affect the planning for and development of hazardous waste management facilities...

  13. 40 CFR 271.18 - Coordination with other programs.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 255) as responsible for the development and implementation of State solid waste management plans under....18 Section 271.18 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... program which may affect the planning for and development of hazardous waste management facilities...

  14. 40 CFR 271.18 - Coordination with other programs.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 255) as responsible for the development and implementation of State solid waste management plans under....18 Section 271.18 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... program which may affect the planning for and development of hazardous waste management facilities...

  15. 40 CFR 271.18 - Coordination with other programs.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 255) as responsible for the development and implementation of State solid waste management plans under....18 Section 271.18 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES... program which may affect the planning for and development of hazardous waste management facilities...

  16. 12 CFR 271.1 - Authority and purpose.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) FEDERAL OPEN MARKET COMMITTEE RULES REGARDING AVAILABILITY OF INFORMATION § 271.1 Authority and purpose. (a) Authority. This part is issued by the Federal... also pursuant to the Committee's authority under section 12A of the Federal Reserve Act, 12 U.S.C. 263...

  17. 12 CFR 271.1 - Authority and purpose.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) FEDERAL OPEN MARKET COMMITTEE RULES REGARDING AVAILABILITY OF INFORMATION § 271.1 Authority and purpose. (a) Authority. This part is issued by the Federal... also pursuant to the Committee's authority under section 12A of the Federal Reserve Act, 12 U.S.C. 263...

  18. 48 CFR 536.271 - Project labor agreements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Project labor agreements... Construction 536.271 Project labor agreements. (a) Authority. This subpart implements the Presidential memorandum of June 5, 1997, on using project labor agreements (PLAs) on Federal construction projects....

  19. 40 CFR 271.26 - Requirements for used oil management.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 27 2014-07-01 2014-07-01 false Requirements for used oil management... Final Authorization § 271.26 Requirements for used oil management. The State shall have standards for used oil management which are equivalent to 40 CFR part 279. These standards shall include: (a...

  20. 48 CFR 1352.271-90 - Insurance requirements.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... and automobile insurance to insure the risks described in paragraph (c) of clause 1352.271-79. This... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Insurance requirements... Insurance requirements. As prescribed in 48 CFR 1371.121, insert the following clause:...

  1. 48 CFR 1352.271-90 - Insurance requirements.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... and automobile insurance to insure the risks described in paragraph (c) of clause 1352.271-79. This... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Insurance requirements... Insurance requirements. As prescribed in 48 CFR 1371.121, insert the following clause:...

  2. 48 CFR 1352.271-90 - Insurance requirements.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... and automobile insurance to insure the risks described in paragraph (c) of clause 1352.271-79. This... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Insurance requirements... Insurance requirements. As prescribed in 48 CFR 1371.121, insert the following clause:...

  3. 7 CFR 271.7 - Allotment reduction procedures.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ..., suspended, or cancelled to comply with section 18 of the Food Stamp Act of 1977, as amended. The best.... Action to comply with section 18 of the Food Stamp Act of 1977, as amended, may be a suspension or... AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM GENERAL INFORMATION AND DEFINITIONS § 271.7 Allotment...

  4. 7 CFR 271.1 - General purpose and scope.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... the levels of nutrition among low-income households. Section 2 of the Food Stamp Act of 1977 states... AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM GENERAL INFORMATION AND DEFINITIONS § 271.1 General purpose and scope. (a) Purpose of the food stamp program. The food stamp program is designed to promote...

  5. 7 CFR 271.1 - General purpose and scope.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... the levels of nutrition among low-income households. Section 2 of the Food Stamp Act of 1977 states... AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM GENERAL INFORMATION AND DEFINITIONS § 271.1 General purpose and scope. (a) Purpose of the food stamp program. The food stamp program is designed to promote...

  6. 7 CFR 271.7 - Allotment reduction procedures.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ..., suspended, or cancelled to comply with section 18 of the Food Stamp Act of 1977, as amended. The best.... Action to comply with section 18 of the Food Stamp Act of 1977, as amended, may be a suspension or... AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM GENERAL INFORMATION AND DEFINITIONS § 271.7 Allotment...

  7. 7 CFR 271.7 - Allotment reduction procedures.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ..., suspended, or cancelled to comply with section 18 of the Food Stamp Act of 1977, as amended. The best.... Action to comply with section 18 of the Food Stamp Act of 1977, as amended, may be a suspension or... AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM GENERAL INFORMATION AND DEFINITIONS § 271.7 Allotment...

  8. 7 CFR 271.7 - Allotment reduction procedures.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ..., suspended, or cancelled to comply with section 18 of the Food Stamp Act of 1977, as amended. The best.... Action to comply with section 18 of the Food Stamp Act of 1977, as amended, may be a suspension or... AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM GENERAL INFORMATION AND DEFINITIONS § 271.7 Allotment...

  9. 7 CFR 271.1 - General purpose and scope.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... the levels of nutrition among low-income households. Section 2 of the Food Stamp Act of 1977 states... AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM GENERAL INFORMATION AND DEFINITIONS § 271.1 General purpose and scope. (a) Purpose of the food stamp program. The food stamp program is designed to promote...

  10. 48 CFR 719.271-6 - Small business screening procedure.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Small business screening... DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-6 Small business screening...) Preparation of Form USAID 1410-14 (the Small Business/Minority Business Enterprise Procurement Review...

  11. 48 CFR 719.271-4 - Heads of contracting activities.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Heads of contracting... DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-4 Heads of contracting activities... is required. The heads of the contracting activities shall be responsible for: (a)...

  12. 48 CFR 218.271 - Head of contracting activity determinations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 3 2014-10-01 2014-10-01 false Head of contracting... Emergency Acquisition Flexibilities 218.271 Head of contracting activity determinations. For contract..., biological, chemical, or radiological attack, the term “head of the agency” is replaced with “head of...

  13. 48 CFR 218.271 - Head of contracting activity determinations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 3 2013-10-01 2013-10-01 false Head of contracting... Emergency Acquisition Flexibilities 218.271 Head of contracting activity determinations. For contract..., biological, chemical, or radiological attack, the term “head of the agency” is replaced with “head of...

  14. 48 CFR 218.271 - Head of contracting activity determinations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 3 2012-10-01 2012-10-01 false Head of contracting... Emergency Acquisition Flexibilities 218.271 Head of contracting activity determinations. For contract..., biological, chemical, or radiological attack, the term “head of the agency” is replaced with “head of...

  15. 48 CFR 719.271-4 - Heads of contracting activities.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Heads of contracting... DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-4 Heads of contracting activities... is required. The heads of the contracting activities shall be responsible for: (a)...

  16. 48 CFR 719.271-4 - Heads of contracting activities.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Heads of contracting... DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-4 Heads of contracting activities... is required. The heads of the contracting activities shall be responsible for: (a)...

  17. 48 CFR 719.271-4 - Heads of contracting activities.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Heads of contracting... DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-4 Heads of contracting activities... is required. The heads of the contracting activities shall be responsible for: (a)...

  18. 48 CFR 218.271 - Head of contracting activity determinations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 3 2011-10-01 2011-10-01 false Head of contracting... Emergency Acquisition Flexibilities 218.271 Head of contracting activity determinations. For contract..., biological, chemical, or radiological attack, the term “head of the agency” is replaced with “head of...

  19. 48 CFR 719.271-4 - Heads of contracting activities.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Heads of contracting... DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-4 Heads of contracting activities... is required. The heads of the contracting activities shall be responsible for: (a)...

  20. 29 CFR 500.271 - Certification of official record.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... MIGRANT AND SEASONAL AGRICULTURAL WORKER PROTECTION Administrative Proceedings Record § 500.271 Certification of official record. Upon receipt of timely notice of appeal to a United States District Court... and file with the appropriate United States District Court, a full, true, and correct copy of...

  1. 48 CFR 642.271 - Government Technical Monitor (GTM).

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 4 2013-10-01 2013-10-01 false Government Technical... MANAGEMENT CONTRACT ADMINISTRATION AND AUDIT SERVICES Contract Administration Services 642.271 Government Technical Monitor (GTM). (a) Policy. The contracting officer may appoint a Government Technical Monitor...

  2. 48 CFR 642.271 - Government Technical Monitor (GTM).

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Government Technical... MANAGEMENT CONTRACT ADMINISTRATION AND AUDIT SERVICES Contract Administration Services 642.271 Government Technical Monitor (GTM). (a) Policy. The contracting officer may appoint a Government Technical Monitor...

  3. 48 CFR 642.271 - Government Technical Monitor (GTM).

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 4 2014-10-01 2014-10-01 false Government Technical... MANAGEMENT CONTRACT ADMINISTRATION AND AUDIT SERVICES Contract Administration Services 642.271 Government Technical Monitor (GTM). (a) Policy. The contracting officer may appoint a Government Technical Monitor...

  4. 48 CFR 642.271 - Government Technical Monitor (GTM).

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 4 2012-10-01 2012-10-01 false Government Technical... MANAGEMENT CONTRACT ADMINISTRATION AND AUDIT SERVICES Contract Administration Services 642.271 Government Technical Monitor (GTM). (a) Policy. The contracting officer may appoint a Government Technical Monitor...

  5. Contextual view of building 271 from across the river shown ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Contextual view of building 271 from across the river shown at center between coal sheds at left and building 91 at right; camera facing northwest. - Mare Island Naval Shipyard, Mechanics Shop, Waterfront Avenue, west side between A Street & Third Street, Vallejo, Solano County, CA

  6. 7 CFR 271.7 - Allotment reduction procedures.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM GENERAL INFORMATION AND DEFINITIONS § 271.7 Allotment..., suspended, or cancelled to comply with section 18 of the Food and Nutrition Act of 2008, as amended....

  7. 14 CFR 121.271 - Fire-extinguishing system materials.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... materials. (b) Connections that are subject to relative motion between components of the airplane must be... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Fire-extinguishing system materials. 121....271 Fire-extinguishing system materials. (a) Except as provided in paragraph (b) of this section,...

  8. 14 CFR 121.271 - Fire-extinguishing system materials.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... materials. (b) Connections that are subject to relative motion between components of the airplane must be... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Fire-extinguishing system materials. 121....271 Fire-extinguishing system materials. (a) Except as provided in paragraph (b) of this section,...

  9. 14 CFR 121.271 - Fire-extinguishing system materials.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Fire-extinguishing system materials. 121....271 Fire-extinguishing system materials. (a) Except as provided in paragraph (b) of this section, each component of a fire-extinguishing system that is in a designated fire zone must be made of...

  10. 48 CFR 719.271-3 - USAID contracting officers.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false USAID contracting officers... DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-3 USAID contracting officers. With... the Federal Acquisition Regulations; (g) Submitting proposed procurement actions for USAID/Washington...

  11. 48 CFR 719.271-3 - USAID contracting officers.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false USAID contracting officers... DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-3 USAID contracting officers. With... the Federal Acquisition Regulations; (g) Submitting proposed procurement actions for USAID/Washington...

  12. 48 CFR 719.271-3 - USAID contracting officers.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false USAID contracting officers... DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-3 USAID contracting officers. With... the Federal Acquisition Regulations; (g) Submitting proposed procurement actions for USAID/Washington...

  13. 48 CFR 719.271-3 - USAID contracting officers.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false USAID contracting officers... DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-3 USAID contracting officers. With... the Federal Acquisition Regulations; (g) Submitting proposed procurement actions for USAID/Washington...

  14. 36 CFR 271.7 - Power to revoke.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... fostering public information in the prevention of forest fires. All authorities and licenses granted under... use involved is injurious to the purpose of forest fire prevention, is offensive to decency or good... 36 Parks, Forests, and Public Property 2 2014-07-01 2014-07-01 false Power to revoke. 271.7...

  15. 36 CFR 271.7 - Power to revoke.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... fostering public information in the prevention of forest fires. All authorities and licenses granted under... use involved is injurious to the purpose of forest fire prevention, is offensive to decency or good... 36 Parks, Forests, and Public Property 2 2013-07-01 2013-07-01 false Power to revoke. 271.7...

  16. 36 CFR 271.7 - Power to revoke.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... fostering public information in the prevention of forest fires. All authorities and licenses granted under... use involved is injurious to the purpose of forest fire prevention, is offensive to decency or good... 36 Parks, Forests, and Public Property 2 2012-07-01 2012-07-01 false Power to revoke. 271.7...

  17. 48 CFR 538.271 - MAS contract awards.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false MAS contract awards. 538... Schedules 538.271 MAS contract awards. (a) MAS awards will be for commercial items as defined in FAR 2.101. Negotiate contracts as a discount from established catalog prices. (b) Before awarding any MAS contract...

  18. 48 CFR 719.271-6 - Small business screening procedure.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Small business screening... DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-6 Small business screening...) Preparation of Form USAID 1410-14 (the Small Business/Minority Business Enterprise Procurement Review...

  19. 48 CFR 719.271-6 - Small business screening procedure.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Small business screening... DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-6 Small business screening...) Preparation of Form USAID 1410-14 (the Small Business/Minority Business Enterprise Procurement Review Form...

  20. 48 CFR 719.271-6 - Small business screening procedure.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Small business screening... DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 719.271-6 Small business screening...) Preparation of Form USAID 1410-14 (the Small Business/Minority Business Enterprise Procurement Review Form...

  1. 50 CFR 622.271 - Recordkeeping and reporting.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... ATLANTIC Dolphin and Wahoo Fishery Off the Atlantic States § 622.271 Recordkeeping and reporting. (a... a commercial permit for Atlantic dolphin and wahoo has been issued, as required under § 622.270(a)(1), or whose vessel fishes for or lands Atlantic dolphin or wahoo in or from state waters adjoining...

  2. 50 CFR 622.271 - Recordkeeping and reporting.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... ATLANTIC Dolphin and Wahoo Fishery Off the Atlantic States § 622.271 Recordkeeping and reporting. (a... a commercial permit for Atlantic dolphin and wahoo has been issued, as required under § 622.270(a)(1), or whose vessel fishes for or lands Atlantic dolphin or wahoo in or from state waters adjoining...

  3. CD271 mediates stem cells to early progeny transition in human epidermis.

    PubMed

    Truzzi, Francesca; Saltari, Annalisa; Palazzo, Elisabetta; Lotti, Roberta; Petrachi, Tiziana; Dallaglio, Katiuscia; Gemelli, Claudia; Grisendi, Giulia; Dominici, Massimo; Pincelli, Carlo; Marconi, Alessandra

    2015-03-01

    CD271 is the low-affinity neurotrophin (p75NTR) receptor that belongs to the tumor necrosis factor receptor superfamily. Because in human epidermis, CD271 is predominantly expressed in transit-amplifying (TA) cells, we evaluated the role of this receptor in keratinocyte differentiation and in the transition from keratinocyte stem cells (KSCs) to progeny. Calcium induced an upregulation of CD271 in subconfluent keratinocytes, which was prevented by CD271 small interfering RNA. Furthermore, CD271 overexpression provoked the switch of KSCs to TA cells, whereas silencing CD271 induced TA cells to revert to a KSC phenotype, as shown by the expression of β1-integrin and by the increased clonogenic ability. CD271(+) keratinocytes sorted from freshly isolated TA cells expressed more survivin and keratin 15 (K15) compared with CD271(-) cells and displayed a higher proliferative capacity. Early differentiation markers and K15 were more expressed in the skin equivalent generated from CD271(+) TA than from those derived from CD271(-) TA cells. By contrast, late differentiation markers were more expressed in skin equivalents from CD271(-) than in reconstructs from CD271(+) TA cells. Finally, skin equivalents originated from CD271(-) TA cells displayed a psoriatic phenotype. These results indicate that CD271 is critical for keratinocyte differentiation and regulates the transition from KSCs to TA cells.

  4. CD271(+) stromal cells expand in arthritic synovium and exhibit a proinflammatory phenotype.

    PubMed

    Del Rey, Manuel J; Faré, Regina; Usategui, Alicia; Cañete, Juan D; Bravo, Beatriz; Galindo, María; Criado, Gabriel; Pablos, José L

    2016-03-15

    CD271(+) stromal cells (SCs) with multipotent stem cell capacity have been identified in synovial tissues, but their functional significance is unknown. We analyzed the distribution of CD271(+) cells in inflammatory synovial tissues as well as their ex vivo immunomodulatory and inflammatory phenotypes. CD271 expression was analyzed by immunohistochemistry in synovial tissues and by flow cytometry in primary adherent synovial cell cultures from rheumatoid arthritis (RA), osteoarthritis (OA), and non-inflammatory control tissues. Isolation of CD271(+) synovial SCs was carried out by magnetic cell sorting. Allogeneic T-cell/SC cocultures were performed to analyze the regulatory capacity of these cells on T-cell proliferation and cytokine production. The production of inflammatory mediators was analyzed in cultures of sorted CD271(+)/(-) SCs. The capacity of CD271(+)/(-) SCs to induce inflammatory cell recruitment in vivo was evaluated in subcutaneous implants in immunodeficient mice. CD271(+) SC were detected in non-inflammatory as well as in arthritic synovial tissues with a specific perivascular distribution. CD271(+) SC density was increased in RA and OA compared with normal synovial tissues. T-cell proliferation and cytokine synthesis were similarly modified by CD271(+) and CD271(-) SCs. Sorted CD271(+) SCs from OA synovial tissues released significantly more interleukin (IL)-6, matrix metalloproteinase (MMP)-1, and MMP-3 than CD271(-) SCs. In immunodeficient mice, implants of CD271(+) SCs induced significantly higher myeloid cell infiltration than CD271(-) SCs. Our results demonstrate that CD271(+) perivascular SCs expand in RA and OA synovial tissues. CD271(+) cells showed enhanced proinflammatory properties ex vivo and in vivo, whereas immunoregulatory properties were equivalent in CD271(+) and CD271(-) SC.

  5. Isolation and Characterization of CD271⁺ Stem Cells Derived from Sheep Dermal Skin.

    PubMed

    Jahroomishirazi, Roomina; Bader, Augustinus; Ebert, Sabine; Schmidt, Christian; Sedaghati, Bita; Schulz-Siegmund, Michaela; Zscharnack, Matthias

    2014-01-01

    Mesenchymal stem cells (MSCs) have great promise in the field of regenerative medicine due to their differentiation potential into several lineages. Besides the bone marrow, MSCs can be obtained from the dermis, which represents a large stem cell reservoir in the skin. Sheep provide an appropriate large animal model for preclinical studies. In this study, we focused on the isolation and characterization of MSCs from sheep dermis as an alternative to bone marrow MSCs (bmMSCs). Primary ovine cells were obtained from the dermis for comparison with bone marrow. CD271(+)/45(-) dermal MSCs (CD271-dMSCs), which were sorted by flow cytometry, and plastic-adherent bmMSCs were examined for morphology, proliferation and senescence-associated β-galactosidase activity in both low and high oxygen conditions. CD271 expression on cultured cells was assessed by flow cytometry. Adipogenic and osteogenic potentials of CD271-dMSCs were evaluated by oil red O and von Kossa staining. Chondrogenic capacity of CD271-dMSCs and CD271(+)/CD45(-) bone marrow cells (CD271-bmMSCs) was detected using immunohistochemistry and measurement of sulfated glycosaminoglycans. The cell proliferation assay demonstrated no significant difference between CD271-dMSCs and bmMSCs under low oxygen conditions. Cultured CD271-dMSCs revealed much more CD271 expression compared to CD271-bmMSCs. CD271-dMSCs and CD271-bmMSCs showed basically similar expression of the cartilage-specific proteins aggrecan and collagen type II, although with a stronger staining in CD271-bmMSC-derived cultures. Remarkably, there was co-expression of CD271 and aggrecan during chondrogenic differentiation, suggesting an involvement of CD271 in chondrogenesis. Based on these findings, CD271-dMSCs might serve as an appropriate alternative cell source in preclinical research. © 2015 S. Karger AG, Basel.

  6. 27 CFR 24.271 - Payment of tax by return with remittance.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2011-04-01 2011-04-01 false Payment of tax by return with remittance. 24.271 Section 24.271 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX..., 2014. ...

  7. 40 CFR 271.9 - Requirements for identification and listing of hazardous wastes.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... listing of hazardous wastes. 271.9 Section 271.9 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.9 Requirements for identification and listing of hazardous wastes...

  8. 34 CFR 271.31 - How does the Secretary determine the amount of the grant?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 1 2011-07-01 2011-07-01 false How does the Secretary determine the amount of the grant? 271.31 Section 271.31 Education Regulations of the Offices of the Department of Education OFFICE... PROGRAM How Does the Secretary Make a Grant? § 271.31 How does the Secretary determine the amount of the...

  9. 34 CFR 271.31 - How does the Secretary determine the amount of the grant?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 34 Education 1 2013-07-01 2013-07-01 false How does the Secretary determine the amount of the grant? 271.31 Section 271.31 Education Regulations of the Offices of the Department of Education OFFICE... PROGRAM How Does the Secretary Make a Grant? § 271.31 How does the Secretary determine the amount of the...

  10. 40 CFR 271.13 - Requirements with respect to permits and permit applications.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 28 2012-07-01 2012-07-01 false Requirements with respect to permits and permit applications. 271.13 Section 271.13 Protection of Environment ENVIRONMENTAL PROTECTION... PROGRAMS Requirements for Final Authorization § 271.13 Requirements with respect to permits and...

  11. 48 CFR 1352.271-72 - Additional Item Requirements (AIR)-growth work

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Requirements (AIR)-growth work 1352.271-72 Section 1352.271-72 Federal Acquisition Regulations System... Clauses 1352.271-72 Additional Item Requirements (AIR)—growth work As prescribed in 48 CFR 1371.103, insert the following clause: Additional Item Requirements (Air)—Growth Work (APR 2010) (a) This...

  12. 48 CFR 1352.271-72 - Additional Item Requirements (AIR)-growth work

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Requirements (AIR)-growth work 1352.271-72 Section 1352.271-72 Federal Acquisition Regulations System... Clauses 1352.271-72 Additional Item Requirements (AIR)—growth work As prescribed in 48 CFR 1371.103, insert the following clause: Additional Item Requirements (Air)—Growth Work (APR 2010) (a) This...

  13. 48 CFR 211.271 - Elimination of use of class I ozone-depleting substances.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... class I ozone-depleting substances. 211.271 Section 211.271 Federal Acquisition Regulations System... Using and Maintaining Requirements Documents 211.271 Elimination of use of class I ozone-depleting substances. See subpart 223.8 for restrictions on contracting for ozone-depleting substances....

  14. 48 CFR 211.271 - Elimination of use of class I ozone-depleting substances.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... class I ozone-depleting substances. 211.271 Section 211.271 Federal Acquisition Regulations System... Using and Maintaining Requirements Documents 211.271 Elimination of use of class I ozone-depleting substances. See subpart 223.8 for restrictions on contracting for ozone-depleting substances....

  15. 48 CFR 211.271 - Elimination of use of class I ozone-depleting substances.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... class I ozone-depleting substances. 211.271 Section 211.271 Federal Acquisition Regulations System... Using and Maintaining Requirements Documents 211.271 Elimination of use of class I ozone-depleting substances. See subpart 223.8 for restrictions on contracting for ozone-depleting substances....

  16. 48 CFR 211.271 - Elimination of use of class I ozone-depleting substances.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... class I ozone-depleting substances. 211.271 Section 211.271 Federal Acquisition Regulations System... Using and Maintaining Requirements Documents 211.271 Elimination of use of class I ozone-depleting substances. See subpart 223.8 for restrictions on contracting for ozone-depleting substances....

  17. 48 CFR 211.271 - Elimination of use of class I ozone-depleting substances.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... class I ozone-depleting substances. 211.271 Section 211.271 Federal Acquisition Regulations System... Using and Maintaining Requirements Documents 211.271 Elimination of use of class I ozone-depleting substances. See subpart 223.8 for restrictions on contracting for ozone-depleting substances....

  18. 34 CFR 271.30 - How does the Secretary evaluate an application?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 1 2011-07-01 2011-07-01 false How does the Secretary evaluate an application? 271.30... Does the Secretary Make a Grant? § 271.30 How does the Secretary evaluate an application? (a) The Secretary evaluates an application submitted under this part on the basis of the requirements in § 271.20...

  19. 34 CFR 271.30 - How does the Secretary evaluate an application?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 1 2010-07-01 2010-07-01 false How does the Secretary evaluate an application? 271.30... Does the Secretary Make a Grant? § 271.30 How does the Secretary evaluate an application? (a) The Secretary evaluates an application submitted under this part on the basis of the requirements in § 271.20...

  20. 48 CFR 852.271-72 - Time spent by counselee in counseling process.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... connection with the counseling process to supply test results or other information for purposes other than... counseling process. 852.271-72 Section 852.271-72 Federal Acquisition Regulations System DEPARTMENT OF... Clauses 852.271-72 Time spent by counselee in counseling process. As prescribed in 871.212, insert the...

  1. 40 CFR 271.11 - Requirements for transporters of hazardous wastes.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... hazardous wastes. 271.11 Section 271.11 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.11 Requirements for transporters of hazardous wastes. (a) The...

  2. 36 CFR 271.8 - Consultation with Association of State Foresters and the Advertising Council.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 36 Parks, Forests, and Public Property 2 2011-07-01 2011-07-01 false Consultation with Association of State Foresters and the Advertising Council. 271.8 Section 271.8 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE USE OF âSMOKEY BEARâ SYMBOL § 271.8 Consultation with...

  3. 40 CFR 271.11 - Requirements for transporters of hazardous wastes.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... hazardous wastes. 271.11 Section 271.11 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.11 Requirements for transporters of hazardous wastes. (a) The State...

  4. 27 CFR 27.1 - Imported distilled spirits, wines, and beer.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., wines, and beer. 27.1 Section 27.1 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS IMPORTATION OF DISTILLED SPIRITS, WINES, AND BEER Scope of Regulations § 27.1 Imported distilled spirits, wines, and beer. This part, “Importation of...

  5. 27 CFR 27.1 - Imported distilled spirits, wines, and beer.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., wines, and beer. 27.1 Section 27.1 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY ALCOHOL IMPORTATION OF DISTILLED SPIRITS, WINES, AND BEER Scope of Regulations § 27.1 Imported distilled spirits, wines, and beer. This part, “Importation of...

  6. 27 CFR 27.1 - Imported distilled spirits, wines, and beer.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., wines, and beer. 27.1 Section 27.1 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY ALCOHOL IMPORTATION OF DISTILLED SPIRITS, WINES, AND BEER Scope of Regulations § 27.1 Imported distilled spirits, wines, and beer. This part, “Importation of...

  7. 27 CFR 27.1 - Imported distilled spirits, wines, and beer.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., wines, and beer. 27.1 Section 27.1 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS IMPORTATION OF DISTILLED SPIRITS, WINES, AND BEER Scope of Regulations § 27.1 Imported distilled spirits, wines, and beer. This part, “Importation of...

  8. 27 CFR 27.1 - Imported distilled spirits, wines, and beer.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., wines, and beer. 27.1 Section 27.1 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS IMPORTATION OF DISTILLED SPIRITS, WINES, AND BEER Scope of Regulations § 27.1 Imported distilled spirits, wines, and beer. This part, “Importation of...

  9. 48 CFR 852.271-73 - Use and publication of counseling results.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... counseling results. 852.271-73 Section 852.271-73 Federal Acquisition Regulations System DEPARTMENT OF... Clauses 852.271-73 Use and publication of counseling results. As prescribed in 871.212, insert the following clause: Use and Publication of Counseling Results (JAN 2008) The contractor agrees that none of...

  10. 40 CFR 81.271 - Central Arizona Intrastate Air Quality Control Region.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 17 2010-07-01 2010-07-01 false Central Arizona Intrastate Air Quality Control Region. 81.271 Section 81.271 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... Quality Control Regions § 81.271 Central Arizona Intrastate Air Quality Control Region. The Central...

  11. 29 CFR 1952.271 - Where the plan may be inspected.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 9 2010-07-01 2010-07-01 false Where the plan may be inspected. 1952.271 Section 1952.271..., DEPARTMENT OF LABOR (CONTINUED) APPROVED STATE PLANS FOR ENFORCEMENT OF STATE STANDARDS Vermont § 1952.271 Where the plan may be inspected. A copy of the principal documents comprising the plan may be inspected...

  12. Potential Effect of CD271 on Human Mesenchymal Stromal Cell Proliferation and Differentiation.

    PubMed

    Calabrese, Giovanna; Giuffrida, Raffaella; Lo Furno, Debora; Parrinello, Nunziatina Laura; Forte, Stefano; Gulino, Rosario; Colarossi, Cristina; Schinocca, Luciana Rita; Giuffrida, Rosario; Cardile, Venera; Memeo, Lorenzo

    2015-07-09

    The Low-Affinity Nerve Growth Factor Receptor (LNGFR), also known as CD271, is a member of the tumor necrosis factor receptor superfamily. The CD271 cell surface marker defines a subset of multipotential mesenchymal stromal cells and may be used to isolate and enrich cells derived from bone marrow aspirate. In this study, we compare the proliferative and differentiation potentials of CD271+ and CD271- mesenchymal stromal cells. Mesenchymal stromal cells were isolated from bone marrow aspirate and adipose tissue by plastic adherence and positive selection. The proliferation and differentiation potentials of CD271+ and CD271- mesenchymal stromal cells were assessed by inducing osteogenic, adipogenic and chondrogenic in vitro differentiation. Compared to CD271+, CD271- mesenchymal stromal cells showed a lower proliferation rate and a decreased ability to give rise to osteocytes, adipocytes and chondrocytes. Furthermore, we observed that CD271+ mesenchymal stromal cells isolated from adipose tissue displayed a higher efficiency of proliferation and trilineage differentiation compared to CD271+ mesenchymal stromal cells isolated from bone marrow samples, although the CD271 expression levels were comparable. In conclusion, these data show that both the presence of CD271 antigen and the source of mesenchymal stromal cells represent important factors in determining the ability of the cells to proliferate and differentiate.

  13. Potential Effect of CD271 on Human Mesenchymal Stromal Cell Proliferation and Differentiation

    PubMed Central

    Calabrese, Giovanna; Giuffrida, Raffaella; Lo Furno, Debora; Parrinello, Nunziatina Laura; Forte, Stefano; Gulino, Rosario; Colarossi, Cristina; Schinocca, Luciana Rita; Giuffrida, Rosario; Cardile, Venera; Memeo, Lorenzo

    2015-01-01

    The Low-Affinity Nerve Growth Factor Receptor (LNGFR), also known as CD271, is a member of the tumor necrosis factor receptor superfamily. The CD271 cell surface marker defines a subset of multipotential mesenchymal stromal cells and may be used to isolate and enrich cells derived from bone marrow aspirate. In this study, we compare the proliferative and differentiation potentials of CD271+ and CD271− mesenchymal stromal cells. Mesenchymal stromal cells were isolated from bone marrow aspirate and adipose tissue by plastic adherence and positive selection. The proliferation and differentiation potentials of CD271+ and CD271− mesenchymal stromal cells were assessed by inducing osteogenic, adipogenic and chondrogenic in vitro differentiation. Compared to CD271+, CD271− mesenchymal stromal cells showed a lower proliferation rate and a decreased ability to give rise to osteocytes, adipocytes and chondrocytes. Furthermore, we observed that CD271+ mesenchymal stromal cells isolated from adipose tissue displayed a higher efficiency of proliferation and trilineage differentiation compared to CD271+ mesenchymal stromal cells isolated from bone marrow samples, although the CD271 expression levels were comparable. In conclusion, these data show that both the presence of CD271 antigen and the source of mesenchymal stromal cells represent important factors in determining the ability of the cells to proliferate and differentiate. PMID:26184166

  14. Kernphysik Element 110 heißt Darmstadtium

    NASA Astrophysics Data System (ADS)

    Bührke, Thomas

    2004-01-01

    Eine der weitreichendsten Konsequenzen der Speziellen Relativitätstheorie ist das Phänomen der Zeitdilatation. Diese wurde nun am Max-Planck-Institut für Kernphysik mit der bisher höchsten Genauigkeit gemessen [1].

  15. CD271 regulates the proliferation and motility of hypopharyngeal cancer cells.

    PubMed

    Mochizuki, Mai; Tamai, Keiichi; Imai, Takayuki; Sugawara, Sayuri; Ogama, Naoko; Nakamura, Mao; Matsuura, Kazuto; Yamaguchi, Kazunori; Satoh, Kennichi; Sato, Ikuro; Motohashi, Hozumi; Sugamura, Kazuo; Tanaka, Nobuyuki

    2016-07-29

    CD271 (p75 neurotrophin receptor) plays both positive and negative roles in cancer development, depending on the cell type. We previously reported that CD271 is a marker for tumor initiation and is correlated with a poor prognosis in human hypopharyngeal cancer (HPC). To clarify the role of CD271 in HPC, we established HPC cell lines and knocked down the CD271 expression using siRNA. We found that CD271-knockdown completely suppressed the cells' tumor-forming capability both in vivo and in vitro. CD271-knockdown also induced cell-cycle arrest in G0 and suppressed ERK phosphorylation. While treatment with an ERK inhibitor only partially inhibited cell growth, CDKN1C, which is required for maintenance of quiescence, was strongly upregulated in CD271-depleted HPC cells, and the double knockdown of CD271 and CDKN1C partially rescued the cells from G0 arrest. In addition, either CD271 depletion or the inhibition of CD271-RhoA signaling by TAT-Pep5 diminished the in vitro migration capability of the HPC cells. Collectively, CD271 initiates tumor formation by increasing the cell proliferation capacity through CDKN1C suppression and ERK-signaling activation, and by accelerating the migration signaling pathway in HPC.

  16. CD271 regulates the proliferation and motility of hypopharyngeal cancer cells

    PubMed Central

    Mochizuki, Mai; Tamai, Keiichi; Imai, Takayuki; Sugawara, Sayuri; Ogama, Naoko; Nakamura, Mao; Matsuura, Kazuto; Yamaguchi, Kazunori; Satoh, Kennichi; Sato, Ikuro; Motohashi, Hozumi; Sugamura, Kazuo; Tanaka, Nobuyuki

    2016-01-01

    CD271 (p75 neurotrophin receptor) plays both positive and negative roles in cancer development, depending on the cell type. We previously reported that CD271 is a marker for tumor initiation and is correlated with a poor prognosis in human hypopharyngeal cancer (HPC). To clarify the role of CD271 in HPC, we established HPC cell lines and knocked down the CD271 expression using siRNA. We found that CD271-knockdown completely suppressed the cells’ tumor-forming capability both in vivo and in vitro. CD271-knockdown also induced cell-cycle arrest in G0 and suppressed ERK phosphorylation. While treatment with an ERK inhibitor only partially inhibited cell growth, CDKN1C, which is required for maintenance of quiescence, was strongly upregulated in CD271-depleted HPC cells, and the double knockdown of CD271 and CDKN1C partially rescued the cells from G0 arrest. In addition, either CD271 depletion or the inhibition of CD271-RhoA signaling by TAT-Pep5 diminished the in vitro migration capability of the HPC cells. Collectively, CD271 initiates tumor formation by increasing the cell proliferation capacity through CDKN1C suppression and ERK-signaling activation, and by accelerating the migration signaling pathway in HPC. PMID:27469492

  17. Operations Resources Management Career Ladder AFSC 271X2.

    DTIC Science & Technology

    1986-02-01

    Flight Maintenance Dispatcher Subcluster (GRP293, N= O ) I_ K -u o . -w f 30 r -l ~ ~ - .. . - --- __ _ FIGURE 1 AFSC 271X2 CAREER LADDER STRUCTURE...DAFSC 27172 AND 27192AP PER SON NE L (PERCENT MEMBERS PERFORMING) DAFSC DAFSC 27152 27172 TASKS (N=b12) (N=65) DIFFERENCE K F156 PREPARE AND MAINTAIN...UNCLASSIFIED F/ O 5/9 NL Ehhhhhhhmmmu mmmmhmhhu smmhhhhhmmhsm IL 11-m1 1 5 11.4~ MICROCOPY RESOLUTION TEST CHART -’wAf 4UPCIir OF T4%(OR~4I 3 UNITED STATES AIR

  18. 271. MONUMENT AT ENTRANCE TO SENIOR OFFICERS' QUARTERS AREA: A ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    271. MONUMENT AT ENTRANCE TO SENIOR OFFICERS' QUARTERS AREA: A BROWNSTONE CARTOUCHE SALVAGED FROM A BUILDING ERECTED IN 1894-95 BY THE STATE OF RHODE ISLAND AS PART OF ITS QUONSET POINT NATIONAL GUARD RESERVATION AND SET UP AS A FREE-STANDING MONUMENT, SUPPORTED BY BRICK PIERS, WHEN THE NAVAL AIR STATION WAS DEVELOPED IN 1940-41; LOCATED AT NORTHEAST CORNER OF GLENN CURTIS DRIVE AND RANGER STREET. VIEW FROM THE SOUTH. - Quonset Point Naval Air Station, Roger Williams Way, North Kingstown, Washington County, RI

  19. CD271 Expression on Patient Melanoma Cells Is Unstable and Unlinked to Tumorigenicity.

    PubMed

    Boyle, Samantha E; Fedele, Clare G; Corbin, Vincent; Wybacz, Elisha; Szeto, Pacman; Lewin, Jeremy; Young, Richard J; Wong, Annie; Fuller, Robert; Spillane, John; Speakman, David; Donahoe, Simon; Pohl, Miklos; Gyorki, David; Henderson, Michael A; Johnstone, Ricky W; Papenfuss, Anthony T; Shackleton, Mark

    2016-07-01

    The stability of markers that identify cancer cells that propagate disease is important to the outcomes of targeted therapy strategies. In human melanoma, conflicting data exist as to whether hierarchical expression of CD271/p75/NGFR (nerve growth factor receptor) marks cells with enriched tumorigenicity, which would compel their specific targeting in therapy. To test whether these discrepancies relate to differences among groups in assay approaches, we undertook side-by-side testing of published methods of patient-derived melanoma xenografting (PDX), including comparisons of tissue digestion procedures or coinjected Matrigel formulations. We found that CD271(-) and CD271(+) melanoma cells from each of seven patients were similarly tumorigenic, regardless of assay variations. Surprisingly variable CD271 expression patterns were observed in the analyses of sibling PDX tumors (n = 68) grown in the same experiments from either CD271(-) or CD271(+) cells obtained from patients. This indicates unstable intratumoral lineage relationships between CD271(-) and CD271(+) melanoma cells that are inconsistent with classical, epigenetically based theories of disease progression, such as the cancer stem cell and plasticity models. SNP genotyping of pairs of sibling PDX tumors grown from phenotypically identical CD271(-) or CD271(+) cells showed large pairwise differences in copy number (28%-48%). Differences were also apparent in the copy number profiles of CD271(-) and CD271(+) cells purified directly from each of the four melanomas (1.4%-23%). Thus, CD271 expression in patient melanomas is unstable, not consistently linked to increased tumorigenicity and associated with genetic heterogeneity, undermining its use as a marker in clinical studies. Cancer Res; 76(13); 3965-77. ©2016 AACR. ©2016 American Association for Cancer Research.

  20. CD271 Defines a Stem Cell-Like Population in Hypopharyngeal Cancer

    PubMed Central

    Imai, Takayuki; Tamai, Keiichi; Oizumi, Sayuri; Oyama, Kyoko; Yamaguchi, Kazunori; Sato, Ikuro; Satoh, Kennichi; Matsuura, Kazuto; Saijo, Shigeru; Sugamura, Kazuo; Tanaka, Nobuyuki

    2013-01-01

    Cancer stem cells contribute to the malignant phenotypes of a variety of cancers, but markers to identify human hypopharyngeal cancer (HPC) stem cells remain poorly understood. Here, we report that the CD271+ population sorted from xenotransplanted HPCs possesses an enhanced tumor-initiating capability in immunodeficient mice. Tumors generated from the CD271+ cells contained both CD271+ and CD271− cells, indicating that the population could undergo differentiation. Immunohistological analyses of the tumors revealed that the CD271+ cells localized to a perivascular niche near CD34+ vasculature, to invasive fronts, and to the basal layer. In accordance with these characteristics, a stemness marker, Nanog, and matrix metalloproteinases (MMPs), which are implicated in cancer invasion, were significantly up-regulated in the CD271+ compared to the CD271− cell population. Furthermore, using primary HPC specimens, we demonstrated that high CD271 expression was correlated with a poor prognosis for patients. Taken together, our findings indicate that CD271 is a novel marker for HPC stem-like cells and for HPC prognosis. PMID:23626764

  1. 34 CFR 271.31 - How does the Secretary determine the amount of the grant?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 34 Education 1 2012-07-01 2012-07-01 false How does the Secretary determine the amount of the grant? 271.31 Section 271.31 Education Regulations of the Offices of the Department of Education OFFICE... ethnic diversity of the student population of the State; (d) The extent to which the applicant will...

  2. 43 CFR 30.271 - How must the tribe pay for the interests it purchases?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 43 Public Lands: Interior 1 2011-10-01 2011-10-01 false How must the tribe pay for the interests it purchases? 30.271 Section 30.271 Public Lands: Interior Office of the Secretary of the Interior... the tribe pay for the interests it purchases? (a) A tribe must pay the full fair market value of the...

  3. 43 CFR 30.271 - How must the tribe pay for the interests it purchases?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false How must the tribe pay for the interests it purchases? 30.271 Section 30.271 Public Lands: Interior Office of the Secretary of the Interior... the tribe pay for the interests it purchases? (a) A tribe must pay the full fair market value of the...

  4. 12 CFR 271.4 - Records available for public inspection and copying.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... be made available for inspection and copying only at the Federal Reserve Bank where those records are... copying. 271.4 Section 271.4 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) FEDERAL OPEN MARKET.... weekdays, at the Freedom of Information Office of the Board of Governors of the Federal Reserve System...

  5. 12 CFR 271.4 - Records available for public inspection and copying.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... be made available for inspection and copying only at the Federal Reserve Bank where those records are... copying. 271.4 Section 271.4 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) FEDERAL OPEN MARKET.... weekdays, at the Freedom of Information Office of the Board of Governors of the Federal Reserve System...

  6. 49 CFR 234.271 - Insulated rail joints, bond wires, and track connections.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Insulated rail joints, bond wires, and track connections. 234.271 Section 234.271 Transportation Other Regulations Relating to Transportation (Continued... joints, bond wires, and track connections. Insulated rail joints, bond wires, and track connections shall...

  7. 30 CFR 550.271 - For what reasons will BOEM disapprove the DPP or DOCD?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... or DOCD? 550.271 Section 550.271 Mineral Resources BUREAU OF OCEAN ENERGY MANAGEMENT, DEPARTMENT OF... of the following apply: (1) Implementing your DPP or DOCD would cause serious harm or damage to life (including fish and other aquatic life), property, any mineral deposits (in areas leased or not leased),...

  8. 30 CFR 550.271 - For what reasons will BOEM disapprove the DPP or DOCD?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... or DOCD? 550.271 Section 550.271 Mineral Resources BUREAU OF OCEAN ENERGY MANAGEMENT, DEPARTMENT OF... of the following apply: (1) Implementing your DPP or DOCD would cause serious harm or damage to life (including fish and other aquatic life), property, any mineral deposits (in areas leased or not leased),...

  9. 30 CFR 550.271 - For what reasons will BOEM disapprove the DPP or DOCD?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... or DOCD? 550.271 Section 550.271 Mineral Resources BUREAU OF OCEAN ENERGY MANAGEMENT, DEPARTMENT OF... of the following apply: (1) Implementing your DPP or DOCD would cause serious harm or damage to life (including fish and other aquatic life), property, any mineral deposits (in areas leased or not leased),...

  10. 34 CFR 271.20 - What conditions must an applicant meet to obtain funding?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 1 2011-07-01 2011-07-01 false What conditions must an applicant meet to obtain funding? 271.20 Section 271.20 Education Regulations of the Offices of the Department of Education OFFICE OF ELEMENTARY AND SECONDARY EDUCATION, DEPARTMENT OF EDUCATION STATE EDUCATIONAL AGENCY DESEGREGATION...

  11. 40 CFR 271.11 - Requirements for transporters of hazardous wastes.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... requirements for shipments by rail or water equivalent to those under 40 CFR 263.20(e) and (f). (4) For exports... hazardous wastes. 271.11 Section 271.11 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS...

  12. 40 CFR 271.11 - Requirements for transporters of hazardous wastes.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... requirements for shipments by rail or water equivalent to those under 40 CFR 263.20(e) and (f). (4) For exports... hazardous wastes. 271.11 Section 271.11 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS...

  13. 40 CFR 271.11 - Requirements for transporters of hazardous wastes.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... requirements for shipments by rail or water equivalent to those under 40 CFR 263.20(e) and (f). (4) For exports... hazardous wastes. 271.11 Section 271.11 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS...

  14. 49 CFR 40.271 - How are alcohol testing problems corrected?

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 1 2013-10-01 2013-10-01 false How are alcohol testing problems corrected? 40.271... WORKPLACE DRUG AND ALCOHOL TESTING PROGRAMS Problems in Alcohol Testing § 40.271 How are alcohol testing... alcohol test for each employee. (1) If, during or shortly after the testing process, you become aware...

  15. 49 CFR 40.271 - How are alcohol testing problems corrected?

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 1 2012-10-01 2012-10-01 false How are alcohol testing problems corrected? 40.271... WORKPLACE DRUG AND ALCOHOL TESTING PROGRAMS Problems in Alcohol Testing § 40.271 How are alcohol testing... alcohol test for each employee. (1) If, during or shortly after the testing process, you become aware...

  16. 49 CFR 40.271 - How are alcohol testing problems corrected?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 1 2011-10-01 2011-10-01 false How are alcohol testing problems corrected? 40.271... WORKPLACE DRUG AND ALCOHOL TESTING PROGRAMS Problems in Alcohol Testing § 40.271 How are alcohol testing... alcohol test for each employee. (1) If, during or shortly after the testing process, you become aware...

  17. 36 CFR 271.8 - Consultation with Association of State Foresters and the Advertising Council.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... of State Foresters and the Advertising Council. 271.8 Section 271.8 Parks, Forests, and Public... Association of State Foresters and the Advertising Council. These regulations in this part have been issued after consultation with the Association of State Foresters and the Advertising Council....

  18. 26 CFR 1.271-1 - Debts owed by political parties.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 3 2010-04-01 2010-04-01 false Debts owed by political parties. 1.271-1 Section... (CONTINUED) INCOME TAXES Items Not Deductible § 1.271-1 Debts owed by political parties. (a) General rule. In... arose, owed by a political party. For example, it is immaterial that the debt may have arisen as a...

  19. 48 CFR 853.271 - Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs. 853.271 Section 853.271 Federal Acquisition... Guaranty, Education and Vocational Rehabilitation and Counseling Programs. ...

  20. 48 CFR 853.271 - Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs. 853.271 Section 853.271 Federal Acquisition... Guaranty, Education and Vocational Rehabilitation and Counseling Programs. ...

  1. 48 CFR 853.271 - Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs. 853.271 Section 853.271 Federal Acquisition... Guaranty, Education and Vocational Rehabilitation and Counseling Programs. ...

  2. 48 CFR 853.271 - Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs. 853.271 Section 853.271 Federal Acquisition... Guaranty, Education and Vocational Rehabilitation and Counseling Programs. ...

  3. 48 CFR 853.271 - Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Loan Guaranty, Education and Vocational Rehabilitation and Counseling Programs. 853.271 Section 853.271 Federal Acquisition... Guaranty, Education and Vocational Rehabilitation and Counseling Programs. ...

  4. 49 CFR 234.271 - Insulated rail joints, bond wires, and track connections.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 4 2011-10-01 2011-10-01 false Insulated rail joints, bond wires, and track connections. 234.271 Section 234.271 Transportation Other Regulations Relating to Transportation (Continued... joints, bond wires, and track connections. Insulated rail joints, bond wires, and track connections...

  5. 48 CFR 852.271-73 - Use and publication of counseling results.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Use and publication of counseling results. 852.271-73 Section 852.271-73 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Texts of Provisions...

  6. 48 CFR 852.271-70 - Nondiscrimination in services provided to beneficiaries.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Nondiscrimination in services provided to beneficiaries. 852.271-70 Section 852.271-70 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Texts...

  7. 7 CFR 2.71 - Director, Office of Risk Assessment and Cost-Benefit Analysis.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... programs in the food and agriculture sciences (7 U.S.C. 3318). (b) Reservation. The following authority is... 7 Agriculture 1 2011-01-01 2011-01-01 false Director, Office of Risk Assessment and Cost-Benefit Analysis. 2.71 Section 2.71 Agriculture Office of the Secretary of Agriculture DELEGATIONS OF AUTHORITY...

  8. 7 CFR 2.71 - Director, Office of Risk Assessment and Cost-Benefit Analysis.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... programs in the food and agriculture sciences (7 U.S.C. 3318). (b) Reservation. The following authority is... 7 Agriculture 1 2010-01-01 2010-01-01 false Director, Office of Risk Assessment and Cost-Benefit Analysis. 2.71 Section 2.71 Agriculture Office of the Secretary of Agriculture DELEGATIONS OF AUTHORITY...

  9. 43 CFR 30.271 - How must the tribe pay for the interests it purchases?

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 43 Public Lands: Interior 1 2013-10-01 2013-10-01 false How must the tribe pay for the interests it purchases? 30.271 Section 30.271 Public Lands: Interior Office of the Secretary of the Interior... the tribe pay for the interests it purchases? (a) A tribe must pay the full fair market value of...

  10. 43 CFR 30.271 - How must the tribe pay for the interests it purchases?

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 43 Public Lands: Interior 1 2012-10-01 2011-10-01 true How must the tribe pay for the interests it purchases? 30.271 Section 30.271 Public Lands: Interior Office of the Secretary of the Interior INDIAN... tribe pay for the interests it purchases? (a) A tribe must pay the full fair market value of...

  11. 43 CFR 30.271 - How must the tribe pay for the interests it purchases?

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 43 Public Lands: Interior 1 2014-10-01 2014-10-01 false How must the tribe pay for the interests it purchases? 30.271 Section 30.271 Public Lands: Interior Office of the Secretary of the Interior... the tribe pay for the interests it purchases? (a) A tribe must pay the full fair market value of...

  12. 20 CFR 702.271 - Discrimination; against employees who bring proceedings, prohibition and penalty.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... proceedings, prohibition and penalty. 702.271 Section 702.271 Employees' Benefits OFFICE OF WORKERS' COMPENSATION PROGRAMS, DEPARTMENT OF LABOR LONGSHOREMEN'S AND HARBOR WORKERS' COMPENSATION ACT AND RELATED... employee: (i) Has claimed or attempted to claim compensation under this Act; or (ii) has testified or...

  13. 20 CFR 702.271 - Discrimination; against employees who bring proceedings, prohibition and penalty.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... proceedings, prohibition and penalty. 702.271 Section 702.271 Employees' Benefits OFFICE OF WORKERS' COMPENSATION PROGRAMS, DEPARTMENT OF LABOR LONGSHOREMEN'S AND HARBOR WORKERS' COMPENSATION ACT AND RELATED... employee: (i) Has claimed or attempted to claim compensation under this Act; or (ii) has testified or...

  14. 20 CFR 702.271 - Discrimination; against employees who bring proceedings, prohibition and penalty.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... proceedings, prohibition and penalty. 702.271 Section 702.271 Employees' Benefits OFFICE OF WORKERS' COMPENSATION PROGRAMS, DEPARTMENT OF LABOR LONGSHOREMEN'S AND HARBOR WORKERS' COMPENSATION ACT AND RELATED... employee: (i) Has claimed or attempted to claim compensation under this Act; or (ii) has testified or...

  15. 20 CFR 702.271 - Discrimination; against employees who bring proceedings, prohibition and penalty.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... proceedings, prohibition and penalty. 702.271 Section 702.271 Employees' Benefits OFFICE OF WORKERS' COMPENSATION PROGRAMS, DEPARTMENT OF LABOR LONGSHOREMEN'S AND HARBOR WORKERS' COMPENSATION ACT AND RELATED... employee: (i) Has claimed or attempted to claim compensation under this Act; or (ii) has testified or...

  16. 48 CFR 212.271 - Limitation on acquisition of right-hand drive passenger sedans.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... of right-hand drive passenger sedans. 212.271 Section 212.271 Federal Acquisition Regulations System... acquisition of right-hand drive passenger sedans. 10 U.S.C. 2253(a)(2) limits the authority to purchase right-hand drive passenger sedans to a cost of not more than $40,000 per vehicle. ...

  17. 48 CFR 212.271 - Limitation on acquisition of right-hand drive passenger sedans.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... of right-hand drive passenger sedans. 212.271 Section 212.271 Federal Acquisition Regulations System... acquisition of right-hand drive passenger sedans. 10 U.S.C. 2253(a)(2) limits the authority to purchase right-hand drive passenger sedans to a cost of not more than $40,000 per vehicle. ...

  18. 48 CFR 212.271 - Limitation on acquisition of right-hand drive passenger sedans.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... of right-hand drive passenger sedans. 212.271 Section 212.271 Federal Acquisition Regulations System... acquisition of right-hand drive passenger sedans. 10 U.S.C. 2253(a)(2) limits the authority to purchase right-hand drive passenger sedans to a cost of not more than $40,000 per vehicle. ...

  19. 34 CFR 271.20 - What conditions must an applicant meet to obtain funding?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 1 2010-07-01 2010-07-01 false What conditions must an applicant meet to obtain funding? 271.20 Section 271.20 Education Regulations of the Offices of the Department of Education OFFICE OF ELEMENTARY AND SECONDARY EDUCATION, DEPARTMENT OF EDUCATION STATE EDUCATIONAL AGENCY DESEGREGATION...

  20. 48 CFR 719.271-7 - Reports on procurement actions that are exempted from screening.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Reports on procurement actions that are exempted from screening. 719.271-7 Section 719.271-7 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies...

  1. Defective autophagy leads to the suppression of stem-like features of CD271(+) osteosarcoma cells.

    PubMed

    Zhang, Dong; Zhao, Qing; Sun, Hao; Yin, Lijuan; Wu, Jiajun; Xu, Jun; He, Tianxiang; Yang, Chunlei; Liang, Chengwei

    2016-11-18

    As an important stress-response mechanism, autophagy plays crucial role in the tumor formation and drug resistance of cancer cells including osteosarcoma (OS). OS cancer stem cells (CSCs) also are considered a key factor of tumorigenesis, drug resistance and tumor recurrence. However, the relationship between autophagy and OS CSCs still remains unclear. CD271+ OS CSCs and CD271- OS cells were isolated by magnetic activated cell sorting. The autophagy level was evaluated by the mRNA expression of autophagy genes, the protein level of LC3II and p62, and the mean number of GFP-LC3 dot per cell. Lentivirus-delivered specific shRNA was utilized to inhibit the corresponding gene expression. The cell viability was examined with CCK8 assay. The cell proliferation level was detected with BrdU staining assay. Cell death was determined by Annexin V/PI double staining of fluorescence activated cell sorting, lactate dehydrogenase release and caspase-3 activity. Tumorigenicity ability was evaluated by colony and sphere formation assay, the protein expression of stemness markers and tumor formation in nude mice. Our data indicated that CD271+ OS CSCs had a similar basic autophagy level with CD271- OS cells. Autophagy deficiency had no observable effects on the levels of cell proliferation and death both in CD271+ and CD271- OS cells under normal condition. However, CD271+ OS cells showed a higher autophagy activity than CD271- OS cells under hypoxia and low nutrient (LH) condition. Moreover, autophagy-deficient CD271+ OS cells lost the advantage of tolerance to LH condition compared to CD271- OS cells. Meanwhile, autophagy deficiency enhanced the sensitivity to chemotherapeutics in the CD271+ cells to the comparable level in the CD271- cells. More importantly, deficient-autophagy decreased the protein expression of stemness markers and caused the disappearance of the superiority in tumorigenicity in vitro and vivo in CD271+ OS cells. The results above demonstrated that autophagy

  2. Tumor stem cells (CD271, c-kit, SOX10) in Melanomas: prognostic and outcome implications.

    PubMed

    Mohamed, Amr; Gonzalez, Raul S; Lawson, Diane; Wang, Jason; Cohen, Cynthia

    2014-01-01

    Melanoma cells that express stem cell marker CD271 are shown to form tumors when transplanted into nude or immunodeficient mice. These tumors have a higher metastatic potential and worse prognosis than melanomas resulting from transplantation of CD271-negative cells. We studied stem cell markers (CD271, c-kit, SOX1O) in melanomas, correlating their presence with prognostic factors and outcome. A total of 82 melanomas in tissue microarrays were immunostained for CD271, c-kit, and SOX10. Results were correlated with clinicopathologic prognostic parameters (Breslow depth of invasion, Clark level, sentinel lymph node status, and pathologic stage) and outcome (recurrence, metastases, and death). Of the 82 melanomas, CD271 was expressed in 18 (21%), c-kit in 47 (57%), and SOX10 in all (100%). CD271 does show correlation with metastases (P=0.05). c-kit is associated with favorable prognostic parameters [Breslow depth (P<0.001) and pathologic stage (P=0.02)] and with improved outcome [recurrence (P=0.03) and metastases (P=0.004)]. Although SOX10 is a good diagnostic marker, it cannot be used for prognosis because it is expressed in all the melanomas studied. In conclusion, CD271 expression in melanomas is associated with increased frequency of metastases, and c-kit immunoreactivity is associated with favorable prognostic parameters and improved outcome.

  3. The role of the cancer stem cell marker CD271 in DNA damage response and drug resistance of melanoma cells.

    PubMed

    Redmer, T; Walz, I; Klinger, B; Khouja, S; Welte, Y; Schäfer, R; Regenbrecht, C

    2017-01-23

    Several lines of evidence have suggested that stemness and acquired resistance to targeted inhibitors or chemotherapeutics are mechanistically linked. Here we observed high cell surface and total levels of nerve growth factor receptor/CD271, a marker of melanoma-initiating cells, in sub-populations of chemoresistant cell lines. CD271 expression was increased in drug-sensitive cells but not resistant cells in response to DNA-damaging chemotherapeutics etoposide, fotemustine and cisplatin. Comparative analysis of melanoma cells engineered to stably express CD271 or a targeting short hairpin RNA by expression profiling provided numerous genes regulated in a CD271-dependent manner. In-depth analysis of CD271-responsive genes uncovered the association of CD271 with regulation of DNA repair components. In addition, gene set enrichment analysis revealed enrichment of CD271-responsive genes in drug-resistant cells, among them DNA repair components. Moreover, our comparative screen identified the fibroblast growth factor 13 (FGF13) as a target of CD271, highly expressed in chemoresistant cells. Further we show that levels of CD271 determine drug response. Knock-down of CD271 in fotemustine-resistant cells decreased expression of FGF13 and at least partly restored sensitivity to fotemustine. Together, we demonstrate that expression of CD271 is responsible for genes associated with DNA repair and drug response. Further, we identified 110 CD271-responsive genes predominantly expressed in melanoma metastases, among them were NEK2, TOP2A and RAD51AP1 as potential drivers of melanoma metastasis. In addition, we provide mechanistic insight in the regulation of CD271 in response to drugs. We found that CD271 is potentially regulated by p53 and in turn is needed for a proper p53-dependent response to DNA-damaging drugs. In summary, we provide for the first time insight in a CD271-associated signaling network connecting CD271 with DNA repair, drug response and metastasis.

  4. The role of the cancer stem cell marker CD271 in DNA damage response and drug resistance of melanoma cells

    PubMed Central

    Redmer, T; Walz, I; Klinger, B; Khouja, S; Welte, Y; Schäfer, R; Regenbrecht, C

    2017-01-01

    Several lines of evidence have suggested that stemness and acquired resistance to targeted inhibitors or chemotherapeutics are mechanistically linked. Here we observed high cell surface and total levels of nerve growth factor receptor/CD271, a marker of melanoma-initiating cells, in sub-populations of chemoresistant cell lines. CD271 expression was increased in drug-sensitive cells but not resistant cells in response to DNA-damaging chemotherapeutics etoposide, fotemustine and cisplatin. Comparative analysis of melanoma cells engineered to stably express CD271 or a targeting short hairpin RNA by expression profiling provided numerous genes regulated in a CD271-dependent manner. In-depth analysis of CD271-responsive genes uncovered the association of CD271 with regulation of DNA repair components. In addition, gene set enrichment analysis revealed enrichment of CD271-responsive genes in drug-resistant cells, among them DNA repair components. Moreover, our comparative screen identified the fibroblast growth factor 13 (FGF13) as a target of CD271, highly expressed in chemoresistant cells. Further we show that levels of CD271 determine drug response. Knock-down of CD271 in fotemustine-resistant cells decreased expression of FGF13 and at least partly restored sensitivity to fotemustine. Together, we demonstrate that expression of CD271 is responsible for genes associated with DNA repair and drug response. Further, we identified 110 CD271-responsive genes predominantly expressed in melanoma metastases, among them were NEK2, TOP2A and RAD51AP1 as potential drivers of melanoma metastasis. In addition, we provide mechanistic insight in the regulation of CD271 in response to drugs. We found that CD271 is potentially regulated by p53 and in turn is needed for a proper p53-dependent response to DNA-damaging drugs. In summary, we provide for the first time insight in a CD271-associated signaling network connecting CD271 with DNA repair, drug response and metastasis. PMID

  5. 48 CFR 1352.271-82 - Department of Labor occupational safety and health standards for ship repair.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... occupational safety and health standards for ship repair. 1352.271-82 Section 1352.271-82 Federal Acquisition... of Provisions and Clauses 1352.271-82 Department of Labor occupational safety and health standards... Occupational Safety and Health Standards for Ship Repair (APR 2010) The contractor, in performance of all...

  6. 48 CFR 1352.271-82 - Department of Labor occupational safety and health standards for ship repair.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... occupational safety and health standards for ship repair. 1352.271-82 Section 1352.271-82 Federal Acquisition... of Provisions and Clauses 1352.271-82 Department of Labor occupational safety and health standards... Occupational Safety and Health Standards for Ship Repair (APR 2010) The contractor, in performance of all work...

  7. 48 CFR 1352.271-82 - Department of Labor occupational safety and health standards for ship repair.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... occupational safety and health standards for ship repair. 1352.271-82 Section 1352.271-82 Federal Acquisition... of Provisions and Clauses 1352.271-82 Department of Labor occupational safety and health standards... Occupational Safety and Health Standards for Ship Repair (APR 2010) The contractor, in performance of all work...

  8. 48 CFR 1352.271-82 - Department of Labor occupational safety and health standards for ship repair.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... occupational safety and health standards for ship repair. 1352.271-82 Section 1352.271-82 Federal Acquisition... of Provisions and Clauses 1352.271-82 Department of Labor occupational safety and health standards... Occupational Safety and Health Standards for Ship Repair (APR 2010) The contractor, in performance of all work...

  9. 48 CFR 1352.271-82 - Department of Labor occupational safety and health standards for ship repair.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... occupational safety and health standards for ship repair. 1352.271-82 Section 1352.271-82 Federal Acquisition... of Provisions and Clauses 1352.271-82 Department of Labor occupational safety and health standards... Occupational Safety and Health Standards for Ship Repair (APR 2010) The contractor, in performance of all work...

  10. 40 CFR 271.22 - Criteria for withdrawing approval of State programs.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.22 Criteria for withdrawing approval of State programs. (a) The...

  11. 30 CFR 250.271 - For what reasons will MMS disapprove the DPP or DOCD?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF Plans and Information Review and Decision Process for the Dpp Or Docd § 250.271 For what reasons will MMS disapprove the DPP or DOCD? The...

  12. Human melanoma-initiating cells express neural crest nerve growth factor receptor CD271.

    PubMed

    Boiko, Alexander D; Razorenova, Olga V; van de Rijn, Matt; Swetter, Susan M; Johnson, Denise L; Ly, Daphne P; Butler, Paris D; Yang, George P; Joshua, Benzion; Kaplan, Michael J; Longaker, Michael T; Weissman, Irving L

    2010-07-01

    The question of whether tumorigenic cancer stem cells exist in human melanomas has arisen in the last few years. Here we show that in melanomas, tumour stem cells (MTSCs, for melanoma tumour stem cells) can be isolated prospectively as a highly enriched CD271(+) MTSC population using a process that maximizes viable cell transplantation. The tumours sampled in this study were taken from a broad spectrum of sites and stages. High-viability cells isolated by fluorescence-activated cell sorting and re-suspended in a matrigel vehicle were implanted into T-, B- and natural-killer-deficient Rag2(-/-)gammac(-/-) mice. The CD271(+) subset of cells was the tumour-initiating population in 90% (nine out of ten) of melanomas tested. Transplantation of isolated CD271(+) melanoma cells into engrafted human skin or bone in Rag2(-/-)gammac(-/-) mice resulted in melanoma; however, melanoma did not develop after transplantation of isolated CD271(-) cells. We also show that in mice, tumours derived from transplanted human CD271(+) melanoma cells were capable of metastatsis in vivo. CD271(+) melanoma cells lacked expression of TYR, MART1 and MAGE in 86%, 69% and 68% of melanoma patients, respectively, which helps to explain why T-cell therapies directed at these antigens usually result in only temporary tumour shrinkage.

  13. Human Melanoma Initiating Cells Express Neural Crest Nerve Growth Factor Receptor CD271

    PubMed Central

    Boiko, Alexander D.; Razorenova, Olga V.; van de Rijn, Matt; Swetter, Susan M.; Johnson, Denise L.; Ly, Daphne P.; Butler, Paris D.; Yang, George P.; Joshua, Benzion; Kaplan, Michael J.; Longaker, Michael T.; Weissman, Irving L.

    2010-01-01

    The question whether tumorigenic cancer stem cells exist in human melanomas has arisen recently1. Here we show that in melanomas, tumor stem cells (MTSC) can be isolated prospectively as a highly enriched CD271+ MTSC population using a process that maximizes viable cell transplantation1,6. In this study the tumors sampled were taken from a broad spectrum of sites and stages. High viability FACS isolated cells resuspended in a matrigel vehicle were implanted into T, B, and NK deficient Rag2−/− γc−/− mice (RG) mice. The CD271+ subset of cells was the tumor initiating population in 9/10 melanomas tested. Transplantation of isolated melanoma cells into engrafted human skin or bone in RG mice resulted in melanoma from CD271+ but not CD271− cells. We also showed that tumors transplanted by CD271+ patient cells were capable of metastasis in-vivo. Importantly, CD271+ melanoma cells lacked expression of TYR, MART and MAGE in 86%, 69% and 68% of melanoma patients respectively suggesting why T cell therapies directed at these antigens usually result in only temporary tumor shrinkage. PMID:20596026

  14. CD271 Down-Regulation Promotes Melanoma Progression and Invasion in Three-Dimensional Models and in Zebrafish.

    PubMed

    Saltari, Annalisa; Truzzi, Francesca; Quadri, Marika; Lotti, Roberta; Palazzo, Elisabetta; Grisendi, Giulia; Tiso, Natascia; Marconi, Alessandra; Pincelli, Carlo

    2016-10-01

    CD271 is a neurotrophin receptor variably expressed in melanoma. Although contradictory data are reported on its role as a marker of tumor-initiating cells, little is known about its function in tumor progression. CD271 expression was higher in spheroids derived from freshly isolated cells of primary melanomas and in primary WM115 and WM793-B cell lines, and it decreased during progression to advanced stages in cells isolated from metastatic melanomas and in metastatic WM266-4 and 1205Lu cell lines. Moreover, CD271 was scarcely detected in the highly invasive spheroids (SKMEL28 and 1205Lu). CD271, originally expressed in the epidermis of skin reconstructs, disappeared when melanoma started to invade the dermis. SKMEL8 CD271(-) cells showed greater proliferation and invasiveness in vitro and were associated with a higher number of metastases in zebrafish compared with CD271(+) cells. CD271 silencing in WM115 induced a more aggressive phenotype in vitro and in vivo. On the contrary, CD271 overexpression in SKMEL28 cells reduced invasion in vitro, and CD271 overexpressing 1205Lu cells was associated with a lower percentage of metastases in zebrafish. A reduced cell-cell adhesion was also observed in the absence of CD271. Taken together, these results indicate that CD271 loss is critical for melanoma progression and metastasis. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

  15. Mesenchymal stromal cells derived from CD271(+) bone marrow mononuclear cells exert potent allosuppressive properties.

    PubMed

    Kuçi, Zyrafete; Kuçi, Selim; Zircher, Susanne; Koller, Stefanie; Schubert, Ralf; Bönig, Halvard; Henschler, Reinhard; Lieberz, Ralf; Klingebiel, Thomas; Bader, Peter

    2011-11-01

    BACKGROUND AIMS. Because data on the immunosuppressive effect of different subsets of mesenchymal stromal cells (MSC) are sparse, we investigated the molecular and cellular mechanisms underlying the allosuppressive effect of MSC generated from bone marrow CD271(+) cells (CD271-MSC) and asked whether this potential is comparable with that of MSC generated through plastic adherence (PA-MSC). METHODS. The immunosuppressive effect of CD271-MSC on the allogeneic reaction was investigated by mixed lymphocyte reaction (MLR). RESULTS. CD271-MSC significantly suppressed the alloantigen-induced proliferation of mononuclear cells (MNC) of two HLA-disparate donors at all MSC:MNC ratios, 1:1, 1:2 and 1:10. They also demonstrated a significantly higher allosuppression than PA-MSC at an MSC:MNC ratio of 1:1. This inhibitory effect was associated with significantly elevated levels of prostaglandin E2 (PGE2) at ratios of 1:1 and 1:2 (about 4-fold), but not at a ratio of 1:10. Indomethacin, and inhibitor of cyclooxygenase-1 and 2 necessary for the biosynthesis of PGE2, mitigated suppressive effects of CD271-MSC only at a ratio of 1:1, indicating that PGE2 is not involved in MSC-mediated inhibition when allogeneic MNC are in excess. The increase of PGE2 was associated with a significant decrease of pro-inflammatory cytokine levels (interferon-gamma and tumor necrosis-alpha), while no changes in levels of interleukin-10, soluble HLA-G and nitric oxide were observed. In addition, CD271-MSC induced an expansion of highly suppressive naive CD4(+)CD25(high)CD45RA(+)CD62L(+) T-regulatory cells, which may extend their allosuppressive effect. CONCLUSIONS. Our data suggest that CD271-MSC exert potent allosuppressive properties and therefore can be used as a reasonable alternative to PA-MSC for the treatment of patients with graft-versus-host disease.

  16. Single CD271 marker isolates mesenchymal stem cells from human dental pulp.

    PubMed

    Alvarez, Ruth; Lee, Hye-Lim; Hong, Christine; Wang, Cun-Yu

    2015-12-18

    Mesenchymal stem cells (MSCs) are a promising tool in regenerative medicine due to their capacity to differentiate into multiple lineages. In addition to MSCs isolated from bone marrow (BMSCs), adult MSCs are isolated from craniofacial tissues including dental pulp tissues (DPs) using various stem cell surface markers. However, there has been a lack of consensus on a set of surface makers that are reproducibly effective at isolating putative multipotent dental mesenchymal stem cells (DMSCs). In this study, we used different combinations of surface markers (CD51/CD140α, CD271, and STRO-1/CD146) to isolate homogeneous populations of DMSCs from heterogeneous dental pulp cells (DPCs) obtained from DP and compared their capacity to undergo multilineage differentiation. Fluorescence-activated cell sorting revealed that 27.3% of DPCs were CD51(+)/CD140α(+), 10.6% were CD271(+), and 0.3% were STRO-1(+)/CD146(+). Under odontogenic conditions, all three subsets of isolated DMSCs exhibited differentiation capacity into odontogenic lineages. Among these isolated subsets of DMSCs, CD271(+) DMSCs demonstrated the greatest odontogenic potential. While all three combinations of surface markers in this study successfully isolated DMSCs from DPCs, the single CD271 marker presents the most effective stem cell surface marker for identification of DMSCs with high odontogenic potential. Isolated CD271(+) DMSCs could potentially be utilized for future clinical applications in dentistry and regenerative medicine.

  17. CD271+ Bone Marrow Mesenchymal Stem Cells May Provide a Niche for Dormant Mycobacterium tuberculosis

    PubMed Central

    Das, Bikul; Kashino, Suely S.; Pulu, Ista; Kalita, Deepjyoti; Swami, Vijay; Yeger, Herman; Felsher, Dean W.; Campos-Neto, Antonio

    2013-01-01

    Mycobacterium tuberculosis (Mtb) can persist in hostile intracellular microenvironments evading immune cells and drug treatment. However, the protective cellular niches where Mtb persists remain unclear. We report that Mtb may maintain long-term intracellular viability in a human bone marrow (BM)–derived CD271+/CD45− mesenchymal stem cell (BM-MSC) population in vitro. We also report that Mtb resides in an equivalent population of BM-MSCs in a mouse model of dormant tuberculosis infection. Viable Mtb was detected in CD271+/CD45− BM-MSCs isolated from individuals who had successfully completed months of anti-Mtb drug treatment. These results suggest that CD271+ BM-MSCs may provide a long-term protective intracellular niche in the host in which dormant Mtb can reside. PMID:23363977

  18. Intravenous application of CD271-selected mesenchymal stem cells during fracture healing.

    PubMed

    Dreger, Tina; Watson, John T; Akers, Walter; Molligan, Jeremy; Achilefu, Samuel; Schon, Lew C; Zhang, Zijun

    2014-01-01

    Circulating mesenchymal stem cells (MSCs) participate in fracture healing and can be used to enhance fracture healing. This study investigated how CD271-selected MSCs travel in circulation and when is the optimal time to apply MSCs intravenously during fracture healing. Based on the expression of CD271, MSCs were isolated from human bone marrow and labeled with cypate, a near-infrared fluorochrome. A unilateral closed fracture was created at the femur in immunodeficient mice. The cypate-labeled MSCs were injected into the tail vein of the mice at days 1 and 3 after fracture and were tracked by near-infrared imaging. The mice were euthanized at 3 weeks after fracture. Immunohistochemistry was performed to detect human MSCs at the fracture sites. Migration of CD271-selected MSCs, under the influence of stem cell-derived factor-1, was assessed in vitro. Intravenously injected at day 1, but not day 3, after fracture, CD271-selected MSCs accumulated at the fracture sites significantly and lasted for at least 7 days. All fractures, with or without MSC injections, healed in 3 weeks. Human cells were localized at the fracture sites in mice by immunohistochemistry. CD271-selected MSCs migrated toward the medium contained stem cell-derived factor-1 in vitro. After intravenous injection, CD271-selected MSCs were recruited to the fracture sites. The stages of fracture healing influenced the homing of culture-expanded MSCs. In mice, an optimal window of intravenous injection of MSCs was around 24 hours after fracture. Intravenous application of MSCs may serve as a practical route to deliver stem cells for the treatment of fracture nonunion and delayed union.

  19. Intravenous Application of CD271-selected Mesenchymal Stem Cells during Fracture Healing

    PubMed Central

    Dreger, Tina; Watson, John Tracy; DVM, Walter Akers; Molligan, Jeremy; Achilefu, Samuel; Schon, Lew C.; Zhang, Zijun

    2014-01-01

    Objectives Circulating mesenchymal stem cells (MSCs) participate in fracture healing and can be used to enhance fracture healing. This study investigated how CD271-selected MSCs travel in circulation and when it is the optimal time to apply MSCs intravenously during fracture healing. Methods Based on the expression of CD271, MSCs were isolated from human bone marrow and labeled with cypate, a near infrared fluorochrome. A unilateral closed fracture was created at the femur in immunodeficient mice. The cypate-labeled MSCs were injected into the tail vein of the mice at days 1 and 3 after fracture, and were tracked by near infrared imaging. The mice were euthanized at 3 weeks after fracture. Immunohistochemistry was performed to detect human MSCs at the fracture sites. Migration of CD271-selected MSCs, under the influence of stem cell derived factor-1 (SDF-1), was assessed in vitro. Results Intravenously injected at day1, but not day 3, after fracture, CD271-selected MSCs accumulated at the fracture sites significantly and that lasted for at least 7 days. All fractures, with or without MSC injections, healed in 3 weeks. Human cells were localized at the fracture sites in mice by immunohistochemistry. CD271-selected MSCs migrated toward the medium contained SDF-1 in vitro. Conclusions After intravenous injection, CD271-selected MSCs were recruited to fracture sites. The stages of fracture healing influenced the homing of culture-expanded MSCs. In mice, an optimal window of intravenous injection of MSCs was around 24 hours after fracture. Clinical Relevance Intravenous application of MSCs may serve as a practical route to deliver stem cells for the treatment of fracture non-union and delayed union. Levels of evidence Level I PMID:24378433

  20. Increased expression of melanoma stem cell marker CD271 in metastatic melanoma to the brain

    PubMed Central

    Guo, Ruifeng; Fierro-Fine, Amelia; Goddard, Lindsey; Russell, Madison; Chen, Jie; Liu, Cheng Z; Fung, Kar-Ming; Hassell, Lewis A

    2014-01-01

    Human melanoma contains multipotent stem cells that express the neural crest stem cell marker CD271. CD271-expressing melanoma cells in murine xenografts give rise to metastatic tumor. However, a comprehensive clinical investigation of its role in different stages of melanomagenesis has not been well studied. We studied CD271 expression with immunohistochemistry in 11 cases of banal melanocytic nevus, 9 cases of primary cutaneous melanoma, 10 cases of primary mucosal melanoma, 5 cases of metastatic melanoma in regional lymph nodes, and 11 cases of metastatic melanoma in the brain. In addition, 9 cases of metastatic, high-grade adenocarcinomas from breast and lung to the brain were studied as controls. The staining was scored based on the number of positive cells and analyzed by student t-test. All banal melanocytic nevi showed negative to equivocal staining. Primary cutaneous melanomas showed variable patterns, mucosal melanomas were mostly negative, and metastases to lymph nodes ranged from negative to moderate positivity. In contrast, all 11 cases of metastatic melanoma to the brain showed moderate (4 cases) to strong positivity (7 cases). Metastases from lung and breast origin were used as controls and showed negative to weakly positive staining in all but one case. Statistically, CD271 has significantly increased expression in metastatic melanoma to the brain when compared to the other groups studied (P < 0.05). The findings suggest that CD271 expression is specifically increased in metastatic melanoma to the brain. Further prospective study for the role of CD271 in prediction of melanoma brain metastasis as well as prognosis assessment will be of great clinical significance. PMID:25674270

  1. Increased expression of melanoma stem cell marker CD271 in metastatic melanoma to the brain.

    PubMed

    Guo, Ruifeng; Fierro-Fine, Amelia; Goddard, Lindsey; Russell, Madison; Chen, Jie; Liu, Cheng Z; Fung, Kar-Ming; Hassell, Lewis A

    2014-01-01

    Human melanoma contains multipotent stem cells that express the neural crest stem cell marker CD271. CD271-expressing melanoma cells in murine xenografts give rise to metastatic tumor. However, a comprehensive clinical investigation of its role in different stages of melanomagenesis has not been well studied. We studied CD271 expression with immunohistochemistry in 11 cases of banal melanocytic nevus, 9 cases of primary cutaneous melanoma, 10 cases of primary mucosal melanoma, 5 cases of metastatic melanoma in regional lymph nodes, and 11 cases of metastatic melanoma in the brain. In addition, 9 cases of metastatic, high-grade adenocarcinomas from breast and lung to the brain were studied as controls. The staining was scored based on the number of positive cells and analyzed by student t-test. All banal melanocytic nevi showed negative to equivocal staining. Primary cutaneous melanomas showed variable patterns, mucosal melanomas were mostly negative, and metastases to lymph nodes ranged from negative to moderate positivity. In contrast, all 11 cases of metastatic melanoma to the brain showed moderate (4 cases) to strong positivity (7 cases). Metastases from lung and breast origin were used as controls and showed negative to weakly positive staining in all but one case. Statistically, CD271 has significantly increased expression in metastatic melanoma to the brain when compared to the other groups studied (P < 0.05). The findings suggest that CD271 expression is specifically increased in metastatic melanoma to the brain. Further prospective study for the role of CD271 in prediction of melanoma brain metastasis as well as prognosis assessment will be of great clinical significance.

  2. In vitro activity of TP-271 against Mycobacterium abscessus, Mycobacterium fortuitum, and Nocardia species.

    PubMed

    Cynamon, Michael; Jureller, Jeff; Desai, Balaji; Ramachandran, Krithika; Sklaney, Mary; Grossman, Trudy H

    2012-07-01

    The in vitro activities of TP-271, a novel fluorocycline antimicrobial, against 22 isolates of Mycobacterium abscessus, 22 isolates of Mycobacterium fortuitum, and 19 isolates of Nocardia spp. were studied by a microtiter broth dilution method. The MIC(90)s for M. abscessus, M. fortuitum, and Nocardia spp. were 0.5 μg/ml, 0.03 μg/ml, and 8 μg/ml, respectively. TP-271 was significantly more active than the respective control drug in virtually all tests.

  3. LLNL's Response to 9/9/2009 Annual Inspection Report Comments B113 Issue #1 and B271 Issue #2

    SciTech Connect

    Schwartz, W W

    2009-11-19

    This is LLNL's responses to comments in the 9/9/2009 Annual Inspection Report concerning Underground Storage Tank (UST) 113-D1U2 (Issue No.1) at Building 113 and UST 271-D2U1 (Issue No.2) at Building 271. Also provided is the required Application for Underground Storage Tank Modification for USTs 113-D1U2 and 271-D2U1 and the specification sheet for the Phil-Tite spill bucket that is proposed to be installed in the 271-D2U1 sump.

  4. Dermal CD271+ Cells are Closely Associated with Regeneration of the Dermis in the Wound Healing Process.

    PubMed

    Iwata, Yohei; Hasebe, Yuichi; Hasegawa, Seiji; Nakata, Satoru; Yagami, Akiko; Matsunaga, Kayoko; Sugiura, Kazumitsu; Akamatsu, Hirohiko

    2017-05-08

    Stem cells have recently been shown to play important roles in wound healing. The aim of this study was to investigate the role of dermal CD271+ cells in wound healing. Full-thickness wounds were produced on the backs of 5-year-old and 24-week-old mice, and time-course of wound closure, CD271+ cell counts, and gene expression levels were compared. Delayed wound healing was observed in 24-week-old mice. The peak of CD271+ cell increase was delayed in 24-week-old mice, and gene expression levels of growth factors in wounded tissue were significantly increased in 5-year-old mice. Dermal CD271+ cells purified by fluorescence-activated cell sorting (FACS) expressed higher growth factors than CD271- cells, suggesting that CD271+ cells play important roles by producing growth factors. This study also investigated dermal CD271+ cells in patients with chronic skin ulcers. Dermal CD271+ cells in patients were significantly reduced compared with in healthy controls. Thus, dermal CD271+ cells are closely associated with wound healing.

  5. Human CD271-positive melanoma stem cells associated with metastasis establish tumor heterogeneity and long-term growth.

    PubMed

    Civenni, Gianluca; Walter, Anne; Kobert, Nikita; Mihic-Probst, Daniela; Zipser, Marie; Belloni, Benedetta; Seifert, Burkhardt; Moch, Holger; Dummer, Reinhard; van den Broek, Maries; Sommer, Lukas

    2011-04-15

    Human melanoma is composed of distinct cell types reminiscent of neural crest derivatives and contains multipotent cells that express the neural crest stem cell markers CD271(p75(NTR)) and Sox10. When isolated from solid tumors by using a method that leaves intact cell surface epitopes, CD271-positive, but not CD271-negative, cells formed tumors on transplantation into nude or nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice. These tumors fully mirrored the heterogeneity of the parental melanoma and could be passaged more than 5 times. In contrast, in more immunocompromised NOD/SCID/IL2rγ(null) mice, or in natural killer cell-depleted nude or NOD/SCID mice, both CD271-positive and CD271-negative tumor cell fractions established tumors. However, tumors resulting from either fraction did not phenocopy the parental tumors, and tumors derived from the CD271-negative cell fraction could not be passaged multiple times. Together, our findings identify CD271-positive cells as melanoma stem cells. Our observation that a relatively high frequency of CD271/Sox10-positive cells correlates with higher metastatic potential and worse prognosis further supports that CD271-positive cells within human melanoma represent genuine cancer stem cells. ©2011 AACR.

  6. 40 CFR 271.24 - Interim authorization under section 3006(g) of RCRA.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.24 Interim authorization under section 3006(g) of RCRA. (a) Any State which is applying for or has been granted final authorization pursuant to section 3006(b) of RCRA...

  7. 40 CFR 271.13 - Requirements with respect to permits and permit applications.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.13 Requirements with respect to permits and permit applications. (a) State law must require permits for owners and operators of all hazardous waste management...

  8. 40 CFR 271.23 - Procedures for withdrawing approval of State programs.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.23 Procedures for withdrawing approval of State programs. (a) A... conformity with the Act and regulations his decision shall constitute “final agency action” within the...

  9. 40 CFR 271.8 - Memorandum of Agreement with the Regional Administrator.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.8 Memorandum of Agreement with the Regional Administrator. (a) Any..., transporters, and HWM facilities in each year for which the State is operating under final authorization. The...

  10. 40 CFR 81.271 - Central Arizona Intrastate Air Quality Control Region.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 17 2011-07-01 2011-07-01 false Central Arizona Intrastate Air Quality... Quality Control Regions § 81.271 Central Arizona Intrastate Air Quality Control Region. The Central Arizona Intrastate Air Quality Control Region consists of the territorial area encompassed by the...

  11. 40 CFR 81.271 - Central Arizona Intrastate Air Quality Control Region.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 18 2014-07-01 2014-07-01 false Central Arizona Intrastate Air Quality... Quality Control Regions § 81.271 Central Arizona Intrastate Air Quality Control Region. The Central Arizona Intrastate Air Quality Control Region consists of the territorial area encompassed by the...

  12. 40 CFR 81.271 - Central Arizona Intrastate Air Quality Control Region.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 18 2013-07-01 2013-07-01 false Central Arizona Intrastate Air Quality... Quality Control Regions § 81.271 Central Arizona Intrastate Air Quality Control Region. The Central Arizona Intrastate Air Quality Control Region consists of the territorial area encompassed by the...

  13. 40 CFR 81.271 - Central Arizona Intrastate Air Quality Control Region.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 18 2012-07-01 2012-07-01 false Central Arizona Intrastate Air Quality... Quality Control Regions § 81.271 Central Arizona Intrastate Air Quality Control Region. The Central Arizona Intrastate Air Quality Control Region consists of the territorial area encompassed by the...

  14. 12 CFR 271.5 - Records available to the public on request.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ....5 Section 271.5 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) FEDERAL OPEN MARKET COMMITTEE... Committee's operations. (2) The request shall be submitted in writing to the Secretary of the Committee, Federal Open Market Committee, 20th & C Street, N.W., Washington, D.C. 20551; or sent by facsimile to...

  15. 40 CFR 271.12 - Requirements for hazardous waste management facilities.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Requirements for Final Authorization § 271.12 Requirements for hazardous waste management facilities. The State shall have standards for hazardous waste management facilities which are equivalent to 40 CFR parts 264... 40 Protection of Environment 27 2011-07-01 2011-07-01 false Requirements for hazardous...

  16. 40 CFR 271.12 - Requirements for hazardous waste management facilities.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Requirements for Final Authorization § 271.12 Requirements for hazardous waste management facilities. The State shall have standards for hazardous waste management facilities which are equivalent to 40 CFR parts 264... 40 Protection of Environment 26 2010-07-01 2010-07-01 false Requirements for hazardous...

  17. 7 CFR 2.71 - Director, Office of Risk Assessment and Cost-Benefit Analysis.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 1 2012-01-01 2012-01-01 false Director, Office of Risk Assessment and Cost-Benefit... Chief Economist § 2.71 Director, Office of Risk Assessment and Cost-Benefit Analysis. (a) Delegations..., Office of Risk Assessment and Cost-Benefit Analysis: (1) Responsible for assessing the risks to...

  18. 7 CFR 2.71 - Director, Office of Risk Assessment and Cost-Benefit Analysis.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 1 2013-01-01 2013-01-01 false Director, Office of Risk Assessment and Cost-Benefit... Chief Economist § 2.71 Director, Office of Risk Assessment and Cost-Benefit Analysis. (a) Delegations..., Office of Risk Assessment and Cost-Benefit Analysis: (1) Responsible for assessing the risks to...

  19. 7 CFR 2.71 - Director, Office of Risk Assessment and Cost-Benefit Analysis.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 1 2014-01-01 2014-01-01 false Director, Office of Risk Assessment and Cost-Benefit... Chief Economist § 2.71 Director, Office of Risk Assessment and Cost-Benefit Analysis. (a) Delegations..., Office of Risk Assessment and Cost-Benefit Analysis: (1) Responsible for assessing the risks to...

  20. 26. BUILDING NO. 271I, LEAD AZIDE PRIMER BUILDING, WESTERN CORNER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    26. BUILDING NO. 271-I, LEAD AZIDE PRIMER BUILDING, WESTERN CORNER OF BUILDING SHOWING DOORS TO MIXING ROOM NO. 4. HAND CRANK VISIBLE AT RIGHT ROTATED SMALL POTS (CONTAINING LEAD AZIDE) IN MIXING ROOMS. - Picatinny Arsenal, 200 Area, Shell Component Loading, State Route 15 near I-80, Dover, Morris County, NJ

  1. 40 CFR 271.10 - Requirements for generators of hazardous wastes.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 26 2010-07-01 2010-07-01 false Requirements for generators of... for Final Authorization § 271.10 Requirements for generators of hazardous wastes. (a) The State program must cover all generators covered by 40 CFR part 262. States must require new generators to...

  2. 40 CFR 271.10 - Requirements for generators of hazardous wastes.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 28 2012-07-01 2012-07-01 false Requirements for generators of... for Final Authorization § 271.10 Requirements for generators of hazardous wastes. (a) The State program must cover all generators covered by 40 CFR part 262. States must require new generators to...

  3. 40 CFR 271.10 - Requirements for generators of hazardous wastes.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 27 2011-07-01 2011-07-01 false Requirements for generators of... for Final Authorization § 271.10 Requirements for generators of hazardous wastes. (a) The State program must cover all generators covered by 40 CFR part 262. States must require new generators to...

  4. 40 CFR 271.10 - Requirements for generators of hazardous wastes.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 27 2014-07-01 2014-07-01 false Requirements for generators of... for Final Authorization § 271.10 Requirements for generators of hazardous wastes. (a) The State program must cover all generators covered by 40 CFR part 262. States must require new generators to...

  5. 40 CFR 271.10 - Requirements for generators of hazardous wastes.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 28 2013-07-01 2013-07-01 false Requirements for generators of... for Final Authorization § 271.10 Requirements for generators of hazardous wastes. (a) The State program must cover all generators covered by 40 CFR part 262. States must require new generators to...

  6. 48 CFR 236.271 - Cost-plus-fixed-fee contracts.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Cost-plus-fixed-fee... CONTRACTS Special Aspects of Contracting for Construction 236.271 Cost-plus-fixed-fee contracts. Annual military construction appropriations acts restrict the use of cost-plus-fixed-fee contracts (see 216.306(c))....

  7. 40 CFR 271.12 - Requirements for hazardous waste management facilities.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 28 2012-07-01 2012-07-01 false Requirements for hazardous waste... (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.12 Requirements for hazardous waste management facilities. The State...

  8. 40 CFR 271.12 - Requirements for hazardous waste management facilities.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 28 2013-07-01 2013-07-01 false Requirements for hazardous waste... (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.12 Requirements for hazardous waste management facilities. The State...

  9. 40 CFR 271.12 - Requirements for hazardous waste management facilities.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 27 2014-07-01 2014-07-01 false Requirements for hazardous waste... (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.12 Requirements for hazardous waste management facilities. The State...

  10. CD271 as a marker to identify mesenchymal stem cells from diverse sources before culture

    PubMed Central

    Álvarez-Viejo, María; Menéndez-Menéndez, Yolanda; Otero-Hernández, Jesús

    2015-01-01

    Mesenchymal stem cells, due to their characteristics are ideal candidates for cellular therapy. Currently, in culture these cells are defined by their adherence to plastic, specific surface antigen expression and multipotent differentiation potential. However, the in vivo identification of mesenchymal stem cells, before culture, is not so well established. Pre-culture identification markers would ensure higher purity than that obtained with selection based on adherence to plastic. Up until now, CD271 has been described as the most specific marker for the characterization and purification of human bone marrow mesenchymal stem cells. This marker has been shown to be specifically expressed by these cells. Thus, CD271 has been proposed as a versatile marker to selectively isolated and expand multipotent mesenchymal stem cells with both immunosuppressive and lymphohematopoietic engraftment-promoting properties. This review focuses on this marker, specifically on identification of mesenchymal stem cells from different tissues. Literature revision suggests that CD271 should not be defined as a universal marker to identify mesenchymal stem cells before culture from different sources. In the case of bone marrow or adipose tissue, CD271 could be considered a quite suitable marker; however this marker seems to be inadequate for the isolation of mesenchymal stem cells from other tissues such as umbilical cord blood or wharton’s jelly among others. PMID:25815130

  11. 48 CFR 719.271-2 - The USAID Office of Small and Disadvantaged Business Utilization (SDB).

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false The USAID Office of Small... Policies 719.271-2 The USAID Office of Small and Disadvantaged Business Utilization (SDB). (a) SDB is...; (2) Advising and consulting regularly with USAID/Washington procuring activities on all phases of...

  12. 48 CFR 719.271-2 - The USAID Office of Small and Disadvantaged Business Utilization (SDB).

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false The USAID Office of Small... Policies 719.271-2 The USAID Office of Small and Disadvantaged Business Utilization (SDB). (a) SDB is...; (2) Advising and consulting regularly with USAID/Washington procuring activities on all phases of...

  13. 48 CFR 719.271-2 - The USAID Office of Small and Disadvantaged Business Utilization (SDB).

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false The USAID Office of Small... Policies 719.271-2 The USAID Office of Small and Disadvantaged Business Utilization (SDB). (a) SDB is...; (2) Advising and consulting regularly with USAID/Washington procuring activities on all phases of...

  14. 48 CFR 719.271-2 - The USAID Office of Small and Disadvantaged Business Utilization (SDB).

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false The USAID Office of Small... Policies 719.271-2 The USAID Office of Small and Disadvantaged Business Utilization (SDB). (a) SDB is...; (2) Advising and consulting regularly with USAID/Washington procuring activities on all phases of...

  15. 48 CFR 719.271-2 - The USAID Office of Small and Disadvantaged Business Utilization (SDB).

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false The USAID Office of Small... Policies 719.271-2 The USAID Office of Small and Disadvantaged Business Utilization (SDB). (a) SDB is...; (2) Advising and consulting regularly with USAID/Washington procuring activities on all phases of...

  16. Hypoxia-Inducible Factor-1α and CD271 inversely correlate with melanoma invasiveness.

    PubMed

    Marconi, Alessandra; Borroni, Riccardo G; Truzzi, Francesca; Longo, Caterina; Pistoni, Federica; Pellacani, Giovanni; Pincelli, Carlo

    2015-05-01

    Melanoma is characterized, among other features, by microenvironmental factors and by an altered apoptotic machinery. Melanoma cell response to a hypoxic environment is transcriptionally regulated by the Hypoxia-Inducible Factor (HIF)-1α. p75 neurotrophin receptor (p75(NTR) ), also called CD271, mediates apoptosis in several cell systems. The purpose of this study was to analyze the expression of HIF-1α and CD271 in melanomas at different phases of progression, as evaluated by histology and reflectance confocal microscopy (RCM). By RCM, 41.67% tumors were characterized by the presence of a population of dendritic and pleomorphic cells (D+P), corresponding to in situ melanoma; 25% exhibited a predominantly round-cell (RN) proliferation with histologic features of superficial melanoma, and 33.33% showed the presence of cells aggregated in nests (DN), typical of invasive melanoma. HIF-1α was scarcely detected in D+P and in RN melanomas, while it was highly expressed in DN tumors. By contrast, CD271 positive cells were mostly detected in D+P population, and barely observed in the other subtypes. This work demonstrates that CD271 expression inversely correlates with hypoxia in melanoma, and that the two markers may be used in the future as diagnostic/prognostic tools for this neoplasm. © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  17. 40 CFR 271.22 - Criteria for withdrawing approval of State programs.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 28 2012-07-01 2012-07-01 false Criteria for withdrawing approval of... Requirements for Final Authorization § 271.22 Criteria for withdrawing approval of State programs. (a) The...) Failure of the State to promulgate or enact new authorities when necessary; or (ii) Action by a...

  18. 29 CFR 1952.271 - Where the plan may be inspected.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ..., DEPARTMENT OF LABOR (CONTINUED) APPROVED STATE PLANS FOR ENFORCEMENT OF STATE STANDARDS Vermont § 1952.271... 02203; and Office of the Commissioner, Vermont Department of Labor and Industry, National Life Building-Drawer 20, 120 State Street, Montpelier, Vermont 05620-3401. ...

  19. 29 CFR 1952.271 - Where the plan may be inspected.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ..., DEPARTMENT OF LABOR (CONTINUED) APPROVED STATE PLANS FOR ENFORCEMENT OF STATE STANDARDS Vermont § 1952.271... 02203; and Office of the Commissioner, Vermont Department of Labor and Industry, National Life Building-Drawer 20, 120 State Street, Montpelier, Vermont 05620-3401. ...

  20. 29 CFR 1952.271 - Where the plan may be inspected.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ..., DEPARTMENT OF LABOR (CONTINUED) APPROVED STATE PLANS FOR ENFORCEMENT OF STATE STANDARDS Vermont § 1952.271... 02203; and Office of the Commissioner, Vermont Department of Labor and Industry, National Life Building-Drawer 20, 120 State Street, Montpelier, Vermont 05620-3401. ...

  1. 29 CFR 1952.271 - Where the plan may be inspected.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ..., DEPARTMENT OF LABOR (CONTINUED) APPROVED STATE PLANS FOR ENFORCEMENT OF STATE STANDARDS Vermont § 1952.271... 02203; and Office of the Commissioner, Vermont Department of Labor and Industry, National Life Building-Drawer 20, 120 State Street, Montpelier, Vermont 05620-3401. ...

  2. 25 CFR 700.271 - Requests for notification of existence of records: Submission.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false Requests for notification of existence of records... OPERATIONS AND RELOCATION PROCEDURES Privacy Act § 700.271 Requests for notification of existence of records... requires that an individual contact more than two officials concerning the existence of records in the...

  3. 25 CFR 700.271 - Requests for notification of existence of records: Submission.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false Requests for notification of existence of records... OPERATIONS AND RELOCATION PROCEDURES Privacy Act § 700.271 Requests for notification of existence of records... requires that an individual contact more than two officials concerning the existence of records in the...

  4. 25 CFR 700.271 - Requests for notification of existence of records: Submission.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false Requests for notification of existence of records... OPERATIONS AND RELOCATION PROCEDURES Privacy Act § 700.271 Requests for notification of existence of records... requires that an individual contact more than two officials concerning the existence of records in the...

  5. 25 CFR 700.271 - Requests for notification of existence of records: Submission.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false Requests for notification of existence of records... OPERATIONS AND RELOCATION PROCEDURES Privacy Act § 700.271 Requests for notification of existence of records... requires that an individual contact more than two officials concerning the existence of records in the...

  6. 25 CFR 700.271 - Requests for notification of existence of records: Submission.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false Requests for notification of existence of records... OPERATIONS AND RELOCATION PROCEDURES Privacy Act § 700.271 Requests for notification of existence of records... requires that an individual contact more than two officials concerning the existence of records in the...

  7. 48 CFR 236.271 - Cost-plus-fixed-fee contracts.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 3 2013-10-01 2013-10-01 false Cost-plus-fixed-fee... CONTRACTS Special Aspects of Contracting for Construction 236.271 Cost-plus-fixed-fee contracts. Annual military construction appropriations acts restrict the use of cost-plus-fixed-fee contracts (see 216.306(c)). ...

  8. 48 CFR 236.271 - Cost-plus-fixed-fee contracts.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 3 2011-10-01 2011-10-01 false Cost-plus-fixed-fee... CONTRACTS Special Aspects of Contracting for Construction 236.271 Cost-plus-fixed-fee contracts. Annual military construction appropriations acts restrict the use of cost-plus-fixed-fee contracts (see 216.306(c)). ...

  9. 48 CFR 236.271 - Cost-plus-fixed-fee contracts.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 3 2014-10-01 2014-10-01 false Cost-plus-fixed-fee... CONTRACTS Special Aspects of Contracting for Construction 236.271 Cost-plus-fixed-fee contracts. Annual military construction appropriations acts restrict the use of cost-plus-fixed-fee contracts (see 216.306(c)). ...

  10. 49 CFR 234.271 - Insulated rail joints, bond wires, and track connections.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 4 2012-10-01 2012-10-01 false Insulated rail joints, bond wires, and track... Inspections and Tests § 234.271 Insulated rail joints, bond wires, and track connections. Insulated rail joints, bond wires, and track connections shall be inspected at least once every three months....

  11. 49 CFR 234.271 - Insulated rail joints, bond wires, and track connections.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 4 2013-10-01 2013-10-01 false Insulated rail joints, bond wires, and track... Inspections and Tests § 234.271 Insulated rail joints, bond wires, and track connections. Insulated rail joints, bond wires, and track connections shall be inspected at least once every three months....

  12. 49 CFR 234.271 - Insulated rail joints, bond wires, and track connections.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 4 2014-10-01 2014-10-01 false Insulated rail joints, bond wires, and track... Inspections and Tests § 234.271 Insulated rail joints, bond wires, and track connections. Insulated rail joints, bond wires, and track connections shall be inspected at least once every three months....

  13. 271. Photocopy of drawing (1981 piping drawing by StearnsRoger Incorporated) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    271. Photocopy of drawing (1981 piping drawing by Stearns-Roger Incorporated) PIPING SCHEMATICS FOR THE ATLAS H CONVERSION, SHEET 517-P1 - Vandenberg Air Force Base, Space Launch Complex 3, Launch Pad 3 East, Napa & Alden Roads, Lompoc, Santa Barbara County, CA

  14. 14 CFR 135.271 - Helicopter hospital emergency medical evacuation service (HEMES).

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ....271 Helicopter hospital emergency medical evacuation service (HEMES). (a) No certificate holder may... assignment, for hospital emergency medical evacuation service helicopter operations unless that assignment... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Helicopter hospital emergency...

  15. 14 CFR 135.271 - Helicopter hospital emergency medical evacuation service (HEMES).

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ....271 Helicopter hospital emergency medical evacuation service (HEMES). (a) No certificate holder may... assignment, for hospital emergency medical evacuation service helicopter operations unless that assignment... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Helicopter hospital emergency...

  16. 14 CFR 135.271 - Helicopter hospital emergency medical evacuation service (HEMES).

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ....271 Helicopter hospital emergency medical evacuation service (HEMES). (a) No certificate holder may... assignment, for hospital emergency medical evacuation service helicopter operations unless that assignment... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Helicopter hospital emergency...

  17. 14 CFR 135.271 - Helicopter hospital emergency medical evacuation service (HEMES).

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ....271 Helicopter hospital emergency medical evacuation service (HEMES). (a) No certificate holder may... assignment, for hospital emergency medical evacuation service helicopter operations unless that assignment... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Helicopter hospital emergency...

  18. 14 CFR 135.271 - Helicopter hospital emergency medical evacuation service (HEMES).

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ....271 Helicopter hospital emergency medical evacuation service (HEMES). (a) No certificate holder may... assignment, for hospital emergency medical evacuation service helicopter operations unless that assignment... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Helicopter hospital emergency...

  19. 48 CFR 1823.271 - NASA Solicitation provision and contract clause.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... AERONAUTICS AND SPACE ADMINISTRATION SOCIOECONOMIC PROGRAMS ENVIRONMENT, ENERGY AND WATER EFFICIENCY, RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND DRUG-FREE WORKPLACE Energy and Water Efficiency and Renewable Energy 1823.271 NASA Solicitation provision and contract clause. Insert the clause at...

  20. 48 CFR 1823.271 - NASA Solicitation provision and contract clause.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... AERONAUTICS AND SPACE ADMINISTRATION SOCIOECONOMIC PROGRAMS ENVIRONMENT, ENERGY AND WATER EFFICIENCY, RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND DRUG-FREE WORKPLACE Energy and Water Efficiency and Renewable Energy 1823.271 NASA Solicitation provision and contract clause. Insert the clause at...

  1. 48 CFR 1823.271 - NASA Solicitation provision and contract clause.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... AERONAUTICS AND SPACE ADMINISTRATION SOCIOECONOMIC PROGRAMS ENVIRONMENT, ENERGY AND WATER EFFICIENCY, RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND DRUG-FREE WORKPLACE Energy and Water Efficiency and Renewable Energy 1823.271 NASA Solicitation provision and contract clause. Insert the clause at...

  2. 48 CFR 1823.271 - NASA Solicitation provision and contract clause.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... AERONAUTICS AND SPACE ADMINISTRATION SOCIOECONOMIC PROGRAMS ENVIRONMENT, ENERGY AND WATER EFFICIENCY, RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND DRUG-FREE WORKPLACE Energy and Water Efficiency and Renewable Energy 1823.271 NASA Solicitation provision and contract clause. Insert the clause at...

  3. 48 CFR 1823.271 - NASA Solicitation provision and contract clause.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... AERONAUTICS AND SPACE ADMINISTRATION SOCIOECONOMIC PROGRAMS ENVIRONMENT, ENERGY AND WATER EFFICIENCY, RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND DRUG-FREE WORKPLACE Energy and Water Efficiency and Renewable Energy 1823.271 NASA Solicitation provision and contract clause. Insert the clause at...

  4. 42 CFR 447.271 - Upper limits based on customary charges.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... SERVICES (CONTINUED) MEDICAL ASSISTANCE PROGRAMS PAYMENTS FOR SERVICES Payment for Inpatient Hospital and Long-Term Care Facility Services Upper Limits § 447.271 Upper limits based on customary charges. (a) The agency may not pay a provider more for inpatient hospital services under Medicaid than...

  5. 48 CFR 2923.271 - Purchase and use of environmentally sound and energy efficient products and services.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ....eere.energy.gov/femp/procurement. www.energystar.gov. (4) The Alternative Fuels Data Center is a one... environmentally sound and energy efficient products and services. 2923.271 Section 2923.271 Federal Acquisition Regulations System DEPARTMENT OF LABOR SOCIOECONOMIC PROGRAMS ENVIRONMENT, ENERGY AND WATER EFFICIENCY...

  6. 48 CFR 2923.271 - Purchase and use of environmentally sound and energy efficient products and services.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ....eere.energy.gov/femp/procurement. www.energystar.gov. (4) The Alternative Fuels Data Center is a one... environmentally sound and energy efficient products and services. 2923.271 Section 2923.271 Federal Acquisition Regulations System DEPARTMENT OF LABOR SOCIOECONOMIC PROGRAMS ENVIRONMENT, ENERGY AND WATER EFFICIENCY...

  7. The Nerve Growth Factor Receptor CD271 Is Crucial to Maintain Tumorigenicity and Stem-Like Properties of Melanoma Cells

    PubMed Central

    Behrens, Diana; Fichtner, Iduna; Przybilla, Dorothea; Wruck, Wasco; Yaspo, Marie-Laure; Lehrach, Hans; Schäfer, Reinhold; Regenbrecht, Christian R. A.

    2014-01-01

    Background Large-scale genomic analyses of patient cohorts have revealed extensive heterogeneity between individual tumors, contributing to treatment failure and drug resistance. In malignant melanoma, heterogeneity is thought to arise as a consequence of the differentiation of melanoma-initiating cells that are defined by cell-surface markers like CD271 or CD133. Results Here we confirmed that the nerve growth factor receptor (CD271) is a crucial determinant of tumorigenicity, stem-like properties, heterogeneity and plasticity in melanoma cells. Stable shRNA mediated knock-down of CD271 in patient-derived melanoma cells abrogated their tumor-initiating and colony-forming capacity. A genome-wide expression profiling and gene-set enrichment analysis revealed novel connections of CD271 with melanoma-associated genes like CD133 and points to a neural crest stem cell (NCSC) signature lost upon CD271 knock-down. In a meta-analysis we have determined a shared set of 271 differentially regulated genes, linking CD271 to SOX10, a marker that specifies the neural crest. To dissect the connection of CD271 and CD133 we have analyzed 10 patient-derived melanoma-cell strains for cell-surface expression of both markers compared to established cell lines MeWo and A375. We found CD271+ cells in the majority of cell strains analyzed as well as in a set of 16 different patient-derived melanoma metastases. Strikingly, only 2/12 cell strains harbored a CD133+ sub-set that in addition comprised a fraction of cells of a CD271+/CD133+ phenotype. Those cells were found in the label-retaining fraction and in vitro deduced from CD271+ but not CD271 knock-down cells. Conclusions Our present study provides a deeper insight into the regulation of melanoma cell properties and points CD271 out as a regulator of several melanoma-associated genes. Further, our data strongly suggest that CD271 is a crucial determinant of stem-like properties of melanoma cells like colony-formation and tumorigenicity

  8. The nerve growth factor receptor CD271 is crucial to maintain tumorigenicity and stem-like properties of melanoma cells.

    PubMed

    Redmer, Torben; Welte, Yvonne; Behrens, Diana; Fichtner, Iduna; Przybilla, Dorothea; Wruck, Wasco; Yaspo, Marie-Laure; Lehrach, Hans; Schäfer, Reinhold; Regenbrecht, Christian R A

    2014-01-01

    Large-scale genomic analyses of patient cohorts have revealed extensive heterogeneity between individual tumors, contributing to treatment failure and drug resistance. In malignant melanoma, heterogeneity is thought to arise as a consequence of the differentiation of melanoma-initiating cells that are defined by cell-surface markers like CD271 or CD133. Here we confirmed that the nerve growth factor receptor (CD271) is a crucial determinant of tumorigenicity, stem-like properties, heterogeneity and plasticity in melanoma cells. Stable shRNA mediated knock-down of CD271 in patient-derived melanoma cells abrogated their tumor-initiating and colony-forming capacity. A genome-wide expression profiling and gene-set enrichment analysis revealed novel connections of CD271 with melanoma-associated genes like CD133 and points to a neural crest stem cell (NCSC) signature lost upon CD271 knock-down. In a meta-analysis we have determined a shared set of 271 differentially regulated genes, linking CD271 to SOX10, a marker that specifies the neural crest. To dissect the connection of CD271 and CD133 we have analyzed 10 patient-derived melanoma-cell strains for cell-surface expression of both markers compared to established cell lines MeWo and A375. We found CD271+ cells in the majority of cell strains analyzed as well as in a set of 16 different patient-derived melanoma metastases. Strikingly, only 2/12 cell strains harbored a CD133+ sub-set that in addition comprised a fraction of cells of a CD271+/CD133+ phenotype. Those cells were found in the label-retaining fraction and in vitro deduced from CD271+ but not CD271 knock-down cells. Our present study provides a deeper insight into the regulation of melanoma cell properties and points CD271 out as a regulator of several melanoma-associated genes. Further, our data strongly suggest that CD271 is a crucial determinant of stem-like properties of melanoma cells like colony-formation and tumorigenicity.

  9. Epigenetic regulation of CD271, a potential cancer stem cell marker associated with chemoresistance and metastatic capacity

    PubMed Central

    LI, SULAN; YUE, DONGLI; CHEN, XINFENG; WANG, LIPING; LI, JIEYAO; PING, YU; GAO, QUN; WANG, DAN; ZHANG, TENGFEI; LI, FENG; YANG, LI; HUANG, LAN; ZHANG, YI

    2015-01-01

    Cancer stem cells (CSCs) are considered to be the cause of tumor initiation, metastasis and recurrence. Additionally, CSCs are responsible for the failure of chemotherapy and radiotherapy. The isolation and identification of CSCs is crucial for facilitating the monitoring, therapy or prevention of cancer. We aimed to identify esophageal squamous cell carcinoma (ESCC) stem-like cells, the epigenetic mechanism and identify novel biomarkers for targeting ESCC CSCs. Sixty-three paired ESCC tissues and adjacent non-cancerous tissues were included in this study. CD271, which was identified as the CSC marker for melanoma, was assessed using quantitative PCR (qPCR). Using flow cytometry, we isolated CD271+ cells comprising 7.5% of cancer cells from the KYSE70 cell line. Sphere formation and anchorage-independent growth were analyzed in CD271+ and CD271− cancer cells, respectively. qPCR was used to detect stem-related genes and CCK-8 was performed to analyze the sensitivity to chemotherapy in the two groups. Bisulfite genomic sequencing was used to analyze the methylation status. CD271 expression was significantly higher in ESCC tissues than in adjacent non-cancerous tissues. Compared with CD271− cancer cells, CD271+ cancer cells showed a higher ability of sphere and colony formation, a high level expression of stem-related gene, and resistance to chemotherapy. The expression of CD271 was induced by a demethylation agent. In conclusion, CD271+ ESCC cells possess stem-like properties. CD271 can potentially act as a prognostic marker for ESCC, whose expression is regulated epigenetically. PMID:25351876

  10. Epigenetic regulation of CD271, a potential cancer stem cell marker associated with chemoresistance and metastatic capacity.

    PubMed

    Li, Sulan; Yue, Dongli; Chen, Xinfeng; Wang, Liping; Li, Jieyao; Ping, Yu; Gao, Qun; Wang, Dan; Zhang, Tengfei; Li, Feng; Yang, Li; Huang, Lan; Zhang, Yi

    2015-01-01

    Cancer stem cells (CSCs) are considered to be the cause of tumor initiation, metastasis and recurrence. Additionally, CSCs are responsible for the failure of chemotherapy and radiotherapy. The isolation and identification of CSCs is crucial for facilitating the monitoring, therapy or prevention of cancer. We aimed to identify esophageal squamous cell carcinoma (ESCC) stem-like cells, the epigenetic mechanism and identify novel biomarkers for targeting ESCC CSCs. Sixty-three paired ESCC tissues and adjacent non-cancerous tissues were included in this study. CD271, which was identified as the CSC marker for melanoma, was assessed using quantitative PCR (qPCR). Using flow cytometry, we isolated CD271+ cells comprising 7.5% of cancer cells from the KYSE70 cell line. Sphere formation and anchorage-independent growth were analyzed in CD271+ and CD271- cancer cells, respectively. qPCR was used to detect stem-related genes and CCK-8 was performed to analyze the sensitivity to chemotherapy in the two groups. Bisulfite genomic sequencing was used to analyze the methylation status. CD271 expression was significantly higher in ESCC tissues than in adjacent non-cancerous tissues. Compared with CD271- cancer cells, CD271+ cancer cells showed a higher ability of sphere and colony formation, a high level expression of stem-related gene, and resistance to chemotherapy. The expression of CD271 was induced by a demethylation agent. In conclusion, CD271+ ESCC cells possess stem-like properties. CD271 can potentially act as a prognostic marker for ESCC, whose expression is regulated epigenetically.

  11. Fluorocycline TP-271 Is Potent against Complicated Community-Acquired Bacterial Pneumonia Pathogens

    PubMed Central

    Fyfe, Corey; O’Brien, William; Hackel, Meredith; Minyard, Mary Beth; Waites, Ken B.; Dubois, Jacques; Murphy, Timothy M.; Slee, Andrew M.; Weiss, William J.; Sutcliffe, Joyce A.

    2017-01-01

    ABSTRACT TP-271 is a novel, fully synthetic fluorocycline antibiotic in clinical development for the treatment of respiratory infections caused by susceptible and multidrug-resistant pathogens. TP-271 was active in MIC assays against key community respiratory Gram-positive and Gram-negative pathogens, including Streptococcus pneumoniae (MIC90 = 0.03 µg/ml), methicillin-sensitive Staphylococcus aureus (MSSA; MIC90 = 0.25 µg/ml), methicillin-resistant S. aureus (MRSA; MIC90 = 0.12 µg/ml), Streptococcus pyogenes (MIC90 = 0.03 µg/ml), Haemophilus influenzae (MIC90 = 0.12 µg/ml), and Moraxella catarrhalis (MIC90 ≤0.016 µg/ml). TP-271 showed activity (MIC90 = 0.12 µg/ml) against community-acquired MRSA expressing Panton-Valentine leukocidin (PVL). MIC90 values against Mycoplasma pneumoniae, Legionella pneumophila, and Chlamydia pneumoniae were 0.004, 1, and 4 µg/ml, respectively. TP-271 was efficacious in neutropenic and immunocompetent animal pneumonia models, generally showing, compared to the burden at the start of dosing, ~2 to 5 log10 CFU reductions against MRSA, S. pneumoniae, and H. influenzae infections when given intravenously (i.v.) and ~1 to 4 log10 CFU reductions when given orally (p.o.). TP-271 was potent against key community-acquired bacterial pneumonia (CABP) pathogens and was minimally affected, or unaffected, by tetracycline-specific resistance mechanisms and fluoroquinolone or macrolide drug resistance phenotypes. IMPORTANCE Rising resistance rates for macrolides, fluoroquinolones, and β-lactams in the most common pathogens associated with community-acquired bacterial pneumonia (CABP) are of concern, especially for cases of moderate to severe infections in vulnerable populations such as the very young and the elderly. New antibiotics that are active against multidrug-resistant Streptococcus pneumoniae and Staphylococcus aureus are needed for use in the empirical treatment of the most severe forms of this disease. TP-271 is a promising

  12. Fluorocycline TP-271 Is Potent against Complicated Community-Acquired Bacterial Pneumonia Pathogens.

    PubMed

    Grossman, Trudy H; Fyfe, Corey; O'Brien, William; Hackel, Meredith; Minyard, Mary Beth; Waites, Ken B; Dubois, Jacques; Murphy, Timothy M; Slee, Andrew M; Weiss, William J; Sutcliffe, Joyce A

    2017-01-01

    TP-271 is a novel, fully synthetic fluorocycline antibiotic in clinical development for the treatment of respiratory infections caused by susceptible and multidrug-resistant pathogens. TP-271 was active in MIC assays against key community respiratory Gram-positive and Gram-negative pathogens, including Streptococcus pneumoniae (MIC90 = 0.03 µg/ml), methicillin-sensitive Staphylococcus aureus (MSSA; MIC90 = 0.25 µg/ml), methicillin-resistant S. aureus (MRSA; MIC90 = 0.12 µg/ml), Streptococcus pyogenes (MIC90 = 0.03 µg/ml), Haemophilus influenzae (MIC90 = 0.12 µg/ml), and Moraxella catarrhalis (MIC90 ≤0.016 µg/ml). TP-271 showed activity (MIC90 = 0.12 µg/ml) against community-acquired MRSA expressing Panton-Valentine leukocidin (PVL). MIC90 values against Mycoplasma pneumoniae, Legionella pneumophila, and Chlamydia pneumoniae were 0.004, 1, and 4 µg/ml, respectively. TP-271 was efficacious in neutropenic and immunocompetent animal pneumonia models, generally showing, compared to the burden at the start of dosing, ~2 to 5 log10 CFU reductions against MRSA, S. pneumoniae, and H. influenzae infections when given intravenously (i.v.) and ~1 to 4 log10 CFU reductions when given orally (p.o.). TP-271 was potent against key community-acquired bacterial pneumonia (CABP) pathogens and was minimally affected, or unaffected, by tetracycline-specific resistance mechanisms and fluoroquinolone or macrolide drug resistance phenotypes. IMPORTANCE Rising resistance rates for macrolides, fluoroquinolones, and β-lactams in the most common pathogens associated with community-acquired bacterial pneumonia (CABP) are of concern, especially for cases of moderate to severe infections in vulnerable populations such as the very young and the elderly. New antibiotics that are active against multidrug-resistant Streptococcus pneumoniae and Staphylococcus aureus are needed for use in the empirical treatment of the most severe forms of this disease. TP-271 is a promising new

  13. Solvent Hold Tank Sample Results for MCU-16-270-271-272: February 2016 Monthly Sample

    SciTech Connect

    Fondeur, F. F.; Jones, D. H.

    2016-05-12

    Savannah River National Lab (SRNL) received one set of Solvent Hold Tank (SHT) samples (MCU-16-270-271-272), pulled on 02/21/2016 for analysis. The samples were combined and analyzed for composition. Analysis of the composite sample MCU-16-270-271-272 indicated the IsoparTML concentration is above its nominal level (101%). The modifier (CS-7SB), the extractant (MaxCalix), and the TiDG concentrations are 7%, 6%, and 40% below their nominal concentrations. This analysis confirms the addition of TiDG, MaxCalix, and modifier to the solvent in November 2015. Based on the current monthly sample, the levels of TiDG, IsoparTML, MaxCalix, and modifier are sufficient for continuing operation but are expected to decrease with time. Periodic characterization and trimming additions to the solvent are recommended.

  14. CD271 is a functional and targetable marker of tumor-initiating cells in head and neck squamous cell carcinoma

    PubMed Central

    Murillo-Sauca, Oihana; Chung, Man Ki; Shin, June Ho; Karamboulas, Christina; Kwok, Shirley; Jung, Young Ho; Oakley, Richard; Tysome, James R.; Farnebo, Lovisa O.; Kaplan, Michael J.; Sirjani, Davud; Divi, Vasu; Holsinger, F. Christopher; Tomeh, Chafeek; Nichols, Anthony; Le, Quynh T.; Colevas, A. A. Dimitrios; Kong, Christina S.; Uppaluri, Ravindra; Lewis, James S.; Ailles, Laurie E.; Sunwoo, John B.

    2014-01-01

    Tumor-initiating cells (TICs) in squamous cell carcinoma of the head and neck (SCCHN) are best characterized by their surface expression of CD44. Although there is great interest in identifying strategies to target this population, no marker of these cells has been found to be functionally active. Here, we examined the expression of the purported marker of normal human oral epithelial stem cells, CD271. We show that CD271 expression is restricted to a subset of the CD44+ cells. Using xenograft assays, we show that the CD44+CD271+ subpopulation contains the most tumorigenic cells. Loss of CD271 function results in a block in the G2-M phase of the cell cycle and a profound negative impact on the capacity of these cells to initiate tumor formation in vivo. Incubation with recombinant NGF results in enhanced phosphorylation of Erk, providing additional evidence that CD271 is functionally active. Finally, incubation of SCCHN cells with antibody to CD271 results in decreased Erk phosphorylation and decreased tumor formation in vivo. Thus, our data are the first to demonstrate that CD271 more specifically identifies the TIC subpopulation within the CD44+ compartment in SCCHN and that this receptor is a functionally active and targetable molecule. PMID:25149537

  15. The Isolated Interacting Galaxy Pair NGC 5426/27 (Arp 271)

    NASA Astrophysics Data System (ADS)

    Fuentes-Carrera, I.; Rosado, M.; Amram, P.; Dultzin-Hacyan, D.; Bernal, A.; Salo, H.; Laurikainen, E.; Cruz-González, I.; Le Coarer, E.

    2001-03-01

    The isolated interacting galaxy pair NGC 5426/27 (Arp 271) was observed using the scanning Fabry-Perot interferometer PUMA. The velocity field, various kinematical parameters and rotation curve for each galaxy were derived. We found a small bar-like structure in NGC 5426 and a severely distorted velocity field for NGC 5427. A range of possible masses was computed for each galaxy.

  16. 27. BUILDING NO. 271I, INTERIOR OF LEAD AZIDE MIXING ROOM. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    27. BUILDING NO. 271-I, INTERIOR OF LEAD AZIDE MIXING ROOM. HERE, TWO POUNDS (MAXIMUM) OF LEAD AZIDE WAS MIXED IN SEVEN SMALL POTS EACH SEPARATED FROM THE OTHERS BY THE STEEL WALLS PICTURED HERE. THE POTS ARE ALL FIXED TO A COMMON SHAFT WHICH IS ROTATED BY A HAND CRANK OUTSIDE OF ROOM (SEE HAER NJ-36A-26). - Picatinny Arsenal, 200 Area, Shell Component Loading, State Route 15 near I-80, Dover, Morris County, NJ

  17. Modifications to the Finnigan MAT 271 mass spectrometer in the Inorganic Gas Analysis Lab

    SciTech Connect

    Reber, S.D.; Cordes, G.T.

    1995-08-01

    This document presents an overview of the modifications that were done to the Finnigan MAT 271 mass spectrometer used in the Dept. 1823 Inorganic Gas Analysis Lab. Among the alterations to the spectrometer were addition of a new computer, interfaces to the power supply, addition of a multimeter and introduction of a Graphical User Interface software system to run the instrument. The impact of these improvements is also discussed. The appendix details a generic procedure for operating the instrument.

  18. Examining the Feasibility of Clinical Grade CD271+ Enrichment of Mesenchymal Stromal Cells for Bone Regeneration

    PubMed Central

    Cuthbert, Richard J.; Giannoudis, Peter V.; Wang, Xiao N.; Nicholson, Lindsay; Pawson, David; Lubenko, Anatole; Tan, Hiang B.; Dickinson, Anne; McGonagle, Dennis; Jones, Elena

    2015-01-01

    Introduction Current clinical trials utilize mesenchymal stromal cells (MSCs) expanded in culture, however these interventions carry considerable costs and concerns pertaining to culture-induced losses of potency. This study assessed the feasibility of new clinical-grade technology to obtain uncultured MSC isolates from three human intra-osseous tissue sources based on immunomagnetic selection for CD271-positive cells. Materials and Methods MSCs were isolated from bone marrow (BM) aspirates or surgical waste materials; enzymatically digested femoral heads (FHs) and reamer irrigator aspirator (RIA) waste fluids. Flow cytometry for the CD45−/lowCD73+CD271+ phenotype was used to evaluate uncultured MSCs before and after selection, and to measure MSC enrichment in parallel to colony forming-unit fibroblast assay. Trilineage differentiation assays and quantitative polymerase chain-reaction for key transcripts involved in bone regeneration was used to assess the functional utility of isolated cells for bone repair. Results Uncultured CD45−/lowCD271+ MSCs uniformly expressed CD73, CD90 and CD105 but showed variable expression of MSCA-1 and SUSD2 (BM>RIA>FH). MSCs were enriched over 150-fold from BM aspirates and RIA fluids, whereas the highest MSC purities were obtained from FH digests. Enriched fractions expressed increased levels of BMP-2, COL1A2, VEGFC, SPARC and CXCL12 transcripts (BM>RIA>FH), with the highest up-regulation detected for CXCL12 in BM (>1300-fold). Following culture expansion, CD271-selected MSCS were tri-potential and phenotypically identical to plastic adherence-selected MSCs. Discussion A CD271-based GMP-compliant immunomagnetic selection resulted in a substantial increase in MSC purity and elevated expression of transcripts involved in bone formation, vascularisation and chemo-attraction. Although this technology, particularly from RIA fluids, can be immediately applied by orthopaedic surgeons as autologous therapy, further improvements in MSC

  19. Acceptance test procedure bldg. 271-U remote monitoring of project W-059 B-Plant canyon exhaust system

    SciTech Connect

    MCDANIEL, K.S.

    1999-09-01

    The test procedure provides for verifying indications and alarms The test procedure provides for verifying indications and alarms associated with the B Plant Canyon Ventilation System as they are being displayed on a remote monitoring workstation located in building 271-U. The system application software was installed by PLCS Plus under contract from B&W Hanford Company. The application software was installed on an existing operator workstation in building 271U which is owned and operated by Bechtel Hanford Inc.

  20. Adventage of mesenchymal stem cells (MSC) expansion directly from purified bone marrow CD105+ and CD271+ cells.

    PubMed

    Jarocha, Danuta; Lukasiewicz, Ewa; Majka, Marcin

    2008-01-01

    Mesenchymal Stem Cells (MSC) are employed in gene and cellular therapies. Routinely MSC are isolated from bone marrow mononuclear cells (MNC) by plastic adherence. Here we compared new isolation strategies of bone marrow MSC including immunodepletion of hematopoietic cells and immunomagnetic isolation of CD105+ and CD271+ populations. Four fractions were obtained: MNC MSC, RosetteSep-isolated MSC, CD105+ and CD271+ sorted MSC. We evaluated i) number of CFU-F colonies, ii) cell phenotype, iii) in vitro differentiation of expanded cells and iv) expression of osteo/adipogenesis related genes. Average number of day 9 CFU-F colonies was the highest for CD271 positive fraction. Real-Time PCR analysis revealed expression of RUNX2, PPARgamma and N-cadherin in isolated cells, particularly high in CD271+ cells. Expression of CD105, CD166, CD44, CD73 antigens was comparable for all expanded populations (over 90%). We observed various levels of hematopoietic contamination with the highest numbers of CD45+ cells in MNC-MSC fraction and the lowest in CD105+ and CD271+ fractions. Cells of all the fractions were CD34 antigen negative. Expanded CD105 and CD271 populations showed higher level of RUNX2, osteocalcin, PTHR, leptin, PPARgamma2 and aggrecan1 genes except for alpha1 collagen. After osteogenic differentiation CD105+ and CD271+ populations showed lower expression of RUNX, PPARgamma2 and also lower expression of osteocalcin and PTHR than MNC, with comparable alpha1-collagen expression. Chondrogenic and adipogenic gene expression was higher in MNC. More clonogenic CD105+ and particularly CD271+ cells, which seem to be the most homogenous fractions based on Real-Time PCR and immunostaining data, are better suited for MSC expansion.

  1. CD271 on melanoma cell is an IFN-γ-inducible immunosuppressive factor that mediates downregulation of melanoma antigens.

    PubMed

    Furuta, Junpei; Inozume, Takashi; Harada, Kazutoshi; Shimada, Shinji

    2014-05-01

    IFN-γ released from cytotoxic T lymphocytes (CTLs) during the effector phase is essential for rejecting bulky melanoma tumors. In contrast, IFN-γ is known to induce certain immunosuppressive factors in tumor cells such as programmed cell death 1 ligand 1 (PD-L1). In this study, we have identified candidates for IFN-γ-inducible CTL-suppressive factors in melanoma cells using complementary DNA microarray analysis, and CD271/p75/neurotrophin receptor (NTR) was one of the candidate genes. Recently, CD271 was identified as a marker of the cancer stem cell-like population in human melanoma tissues. In this study, we showed that overexpression of CD271 on melanoma cells suppressed the in vitro activation of melanoma-specific CTLs. This suppression was mediated by CD271 ligation with activated CTL-derived nerve growth factor and the subsequent downregulation of melanoma antigens. Moreover, we found that the expression levels of PD-L1 on melanoma cells correlated with those of CD271, and they additively suppressed the activation of melanoma-specific CTLs. To the best of our knowledge, the role of overexpression of CD271 in an anti-melanoma T-cell response has been unreported.

  2. Bipolar disorder susceptibility region on Xq24-q27.1 in Finnish families.

    PubMed

    Ekholm, J M; Pekkarinen, P; Pajukanta, P; Kieseppä, T; Partonen, T; Paunio, T; Varilo, T; Perola, M; Lönnqvist, J; Peltonen, L

    2002-01-01

    Bipolar disorder (BPD) is a common disorder characterized by episodes of mania, hypomania and depression. The genetic background of BPD remains undefined, although several putative loci predisposing to BPD have been identified. We have earlier reported significant evidence of linkage for BPD to chromosome Xq24-q27.1 in an extended pedigree from the late settlement region of the genetically isolated population of Finland. Further, we established a distinct chromosomal haplotype covering a 19 cM region on Xq24-q27.1 co-segregating with the disorder. Here, we have further analyzed this X-chromosomal region using a denser marker map and monitored X-chromosomal haplotypes in a study sample of 41 Finnish bipolar families. Only a fraction of the families provided any evidence of linkage to this region, suggesting that a relatively rare gene predisposing to BPD is enriched in this linked pedigree. The genome-wide scan for BPD predisposing loci in this large pedigree indicated that this particular X-chromosomal region provides the best evidence of linkage genome-wide, suggesting an X-chromosomal gene with a major role for the genetic predisposition of BPD in this family.

  3. Adipogenic and osteogenic differentiation of Lin(-)CD271(+)Sca-1(+) adipose-derived stem cells.

    PubMed

    Xiao, Jingang; Yang, Xiaojuan; Jing, Wei; Guo, Weihua; Sun, Qince; Lin, Yunfeng; Liu, Lei; Meng, Wentong; Tian, Weidong

    2013-05-01

    Adipose-derived stem cells (ASCs) have been defined as cells that undergo sustained in vitro growth and have multilineage differentiation potential. However, the identity and purification of ASCs has proved elusive due to the lack of specific markers and poor understanding of their physiological roles. Here, we prospectively isolated and identified a restricted homogeneous subpopulation of ASCs (Lin(-)CD271(+)Sca-1(+)) from mouse adipose tissues on the basis of cell-surface markers. Individual ASCs generated colony-forming unit-fibroblast at a high frequency and could differentiate into adipocytes, osteoblasts, and chondrocytes in vitro. Expansion of ASCs in a large quantity was feasible in medium supplemented with fibroblast growth factor-2 and leukemia inhibitory factor, without loss of adipogenic and osteogenic differentiation capacity. Moreover, we found that the transplanted ASCs can differentiate into adipocytes in adipogenic microenvironment in vivo and osteoblasts in osteogenic microenvironment in vivo. Thus we proved that Lin, CD271, and Sca-1 could be used as the specific markers to purify ASCs from adipose tissue. The method we established to identify ASCs as defined in vivo entities will help develop ASCs transplantation as a new therapeutic strategy for bone regeneration and adipose tissue regeneration in clinic.

  4. CD271+ Mesenchymal Stem Cells as a Possible Infectious Niche for Leishmania infantum

    PubMed Central

    Lopes, Carolina S.; Daifalla, Nada; Das, Bikul; Dias da Silva, Valdo; Campos-Neto, Antonio

    2016-01-01

    Visceral leishmaniasis (VL) is a serious and fatal disease. Therapeutic drugs are toxic and non-sterilizing. The etiological agents Leishmania infantum and Leishmania donovani cause active and asymptomatic diseases. Effective drugs to treat VL exist but unfortunately, post-treatment relapses are common. Little is known why drugs are non-sterilizing or how these intracellular pathogens can escape treatment. Here, using a murine model of VL we found that CD271+/Sca1+ bone marrow mesenchymal stem cells (BM-MSCs) are readily infected in vitro and in vivo by L. infantum. Because BM-MSCs express potent drug efflux pumps, e.g., ABCG2 it is possible that this unique intracellular infectious niche could allow L. infantum to escape anti-parasite drugs. PMID:27622907

  5. Site-directed mutagenesis of Arg60 and Cys271 in ornithine transcarbamylase from rat liver.

    PubMed

    McDowall, S; van Heeswijck, R; Hoogenraad, N

    1990-10-01

    We have investigated the putative carbamylphosphate- and ornithine-binding domains in ornithine transcarbamylase from rat liver using site-directed mutagenesis. Arg60, present in the phosphate-binding motif X-Ser-X-Arg-X and therefore implicated in the binding of the phosphate moiety of carbamylphosphate has been replaced with a leucine. This results in a dramatic reduction of catalytic activity, although the enzyme is synthesized in cells stably transfected with the mutant clone and imported, correctly processed and assembled into a homotrimer in mitochondria. The sole cysteine residue (Cys271) has been implicated in ornithine binding by the chemical modification studies of Marshall and Cohen in 1972 and 1980 (J. Biol. Chem., 247, 1654-1668, 1669-1682; 255, 7291-7295, 7296-7300). Replacement of this residue with serine did not eliminate enzyme activity but affected the Michaelis constant for ornithine (Kb), increasing it 5-fold from 0.71 to 3.7 mM and reduced the kcat at pH 8.5 by 20-fold. These changes represent a loss in apparent binding energy for the enzyme--ornithine complex of 2.9 kcal/mol, suggesting that Cys271 is normally involved in hydrogen bonding to the substrate, ornithine. The cysteine to serine substitution also caused the dissociation constant (Kii) for the competitive inhibitor, L-norvaline to be increased 10-fold, from 12 to 120 microM. The small loss in binding energy and relatively high residual catalytic activity of the mutant strongly suggests that a number of other residues are involved in the binding of ornithine.(ABSTRACT TRUNCATED AT 250 WORDS)

  6. CD271+ Subpopulation of Pancreatic Stellate Cells Correlates with Prognosis of Pancreatic Cancer and Is Regulated by Interaction with Cancer Cells

    PubMed Central

    Fujiwara, Kenji; Ohuchida, Kenoki; Mizumoto, Kazuhiro; Shindo, Koji; Eguchi, Daiki; Kozono, Shingo; Ikenaga, Naoki; Ohtsuka, Takao; Takahata, Shunichi; Aishima, Shinichi; Tanaka, Masao

    2012-01-01

    Pancreatic stellate cells (PSCs) play a crucial role in the aggressive behavior of pancreatic cancer. Although heterogeneity of PSCs has been identified, the functional differences remain unclear. We characterized CD271+ PSCs in human pancreatic cancer. Immunohistochemistry for CD271 was performed for 31 normal pancreatic tissues and 105 pancreatic ductal adenocarcinomas (PDACs). We performed flow cytometry and quantitative RT-PCR, and assessed CD271 expression in PSCs isolated from pancreatic tissues and the changes in CD271 expression in PSCs cocultured with cancer cells. We also investigated the pattern of CD271 expression in a SCID mouse xenograft model. In the immunohistochemical analyses, the CD271-high staining rates in pancreatic stroma in normal pancreatic tissues and PDACs were 2/31 (6.5%) and 29/105 (27.6%), respectively (p = 0.0069). In PDACs, CD271+ stromal cells were frequently observed on the edge rather than the center of the tumors. Stromal CD271 high expression was associated with a good prognosis (p = 0.0040). Flow cytometric analyses demonstrated CD271-positive rates in PSCs were 0–2.1%. Quantitative RT-PCR analyses revealed that CD271 mRNA expression was increased in PSCs after coculture with pancreatic cancer cells. However, the level of CD271 mRNA expression subsequently decreased after the transient increase. Furthermore, CD271 mRNA expression was decreased in PSCs migrating toward pancreatic cancer cells through Matrigel. In the xenograft model, CD271+ PSCs were present at tumor margins/periphery and were absent in the tumor core. In conclusion, CD271 was expressed in PSCs around pancreatic tumors, but not in the center of the tumors, and expression decreased after long coculture with pancreatic cancer cells or after movement toward pancreatic cancer cells. These findings suggest that CD271+ PSCs appear at the early stage of pancreatic carcinogenesis and that CD271 expression is significantly correlated with a better prognosis in

  7. 40 CFR 271.27 - Interim authorization-by-rule for the revised Corrective Action Management Unit rule.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) REQUIREMENTS FOR AUTHORIZATION OF STATE HAZARDOUS WASTE PROGRAMS Requirements for Final Authorization § 271.27 Interim authorization-by-rule for the... State has been granted final authorization pursuant to section 3006(b) of RCRA for the regulation...

  8. 48 CFR 2923.271 - Purchase and use of environmentally sound and energy efficient products and services.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ..., RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND DRUG-FREE WORKPLACE Energy And Water Efficiency and Renewable Energy 2923.271 Purchase and use of environmentally sound and energy efficient products and...-efficient products, alternate fuel vehicles, and products using renewable energy. (1) To the extent...

  9. 48 CFR 2923.271 - Purchase and use of environmentally sound and energy efficient products and services.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ..., RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND DRUG-FREE WORKPLACE Energy And Water Efficiency and Renewable Energy 2923.271 Purchase and use of environmentally sound and energy efficient products and...-efficient products, alternate fuel vehicles, and products using renewable energy. (1) To the extent...

  10. Handbook of Accelerator Physics and Engineering (sections 2.7.1 - 2.7.5 and 7.6.2)

    SciTech Connect

    Roser, T.

    1999-04-19

    The sections written by this author are: 2.7.1- Thomas - BMT equation; 2.2.2- Spinor Algebra; 2.7.3- Spin Rotators and Siberian Snakes; 2.7.4- Ring with Spin Rotator and Siberian Snakes; 2.7.5- Depolarizing Resonances and Spin Flippers; & 7.6.2- Proton Beam Polarimeters

  11. Stem cell marker CD271 is expressed by vasculogenic mimicry-forming uveal melanoma cells in three-dimensional cultures.

    PubMed

    Valyi-Nagy, Klara; Kormos, Bernadett; Ali, Mohamed; Shukla, Deepak; Valyi-Nagy, Tibor

    2012-01-01

    Cancer stem cells have increased resistance against a variety of anti-tumor treatment modalities. Vasculogenic mimicry (VM) patterns are present in numerous malignant tumor types, represent the formation of perfusion pathways by tumor cells, and their presence in tumors is associated with adverse outcome. Earlier we have shown that VM-forming tumor cells in three-dimensional (3D) uveal melanoma cultures have increased resistance against cytotoxic agents and oncolytic herpes simplex virus-mediated destruction. The purpose of the current study was to explore the possibility that this increased resistance of VM-forming tumor cells is due to a cancer stem cell phenotype. The expression of cancer stem cell marker cluster of differentiation 271 (CD271) was determined in traditional two-dimensional (2D) and 3D cultures of C918 uveal melanoma cells by fluorescent immunocytochemistry. We found that the VM-forming tumor cell subpopulation in 3D cultures expressed CD271. In contrast, cells grown in 2D cultures and tumor cell subpopulations not participating in VM formation in 3D cultures were negative for CD271. These findings suggest that VM-forming uveal melanoma cells acquire a cancer stem cell-like phenotype that may play a role in the increased therapy resistance of these cells.

  12. Stem cell marker CD271 is expressed by vasculogenic mimicry-forming uveal melanoma cells in three-dimensional cultures

    PubMed Central

    Valyi-Nagy, Klara; Kormos, Bernadett; Ali, Mohamed; Shukla, Deepak

    2012-01-01

    Purpose Cancer stem cells have increased resistance against a variety of anti-tumor treatment modalities. Vasculogenic mimicry (VM) patterns are present in numerous malignant tumor types, represent the formation of perfusion pathways by tumor cells, and their presence in tumors is associated with adverse outcome. Earlier we have shown that VM-forming tumor cells in three-dimensional (3D) uveal melanoma cultures have increased resistance against cytotoxic agents and oncolytic herpes simplex virus-mediated destruction. The purpose of the current study was to explore the possibility that this increased resistance of VM-forming tumor cells is due to a cancer stem cell phenotype. Methods The expression of cancer stem cell marker cluster of differentiation 271 (CD271) was determined in traditional two-dimensional (2D) and 3D cultures of C918 uveal melanoma cells by fluorescent immunocytochemistry. Results We found that the VM-forming tumor cell subpopulation in 3D cultures expressed CD271. In contrast, cells grown in 2D cultures and tumor cell subpopulations not participating in VM formation in 3D cultures were negative for CD271. Conclusions These findings suggest that VM-forming uveal melanoma cells acquire a cancer stem cell-like phenotype that may play a role in the increased therapy resistance of these cells. PMID:22419851

  13. CD271 antigen defines a subset of multipotent stromal cells with immunosuppressive and lymphohematopoietic engraftment-promoting properties

    PubMed Central

    Kuçi, Selim; Kuçi, Zyrafete; Kreyenberg, Hermann; Deak, Erika; Pütsch, Kathrin; Huenecke, Sabine; Amara, Chandrasekhar; Koller, Stefanie; Rettinger, Eva; Grez, Manuel; Koehl, Ulrike; Latifi-Pupovci, Hatixhe; Henschler, Reinhard; Tonn, Torsten; von Laer, Dorothee; Klingebiel, Thomas; Bader, Peter

    2010-01-01

    Background In vitro proliferative and differentiation potential of mesenchymal stromal cells generated from CD271+ bone marrow mononuclear cells (CD271-mesenchymal stromal cells) has been demonstrated in several earlier and recent reports. In the present study we focused, in addition to proliferative and differentiation potential, on in vitro and in vivo immunosuppressive and lymphohematopoietic engraftment-promoting potential of these mesenchymal stromal cells compared to bone marrow-derived mesenchymal stromal cells generated by plastic adherence (plastic adherence-mesenchymal stromal cells). Design and Methods We set up a series of experimental protocols in order to determine the phenotype of CD271-mesenchymal stromal cells, and their clonogenic, proliferative, differentiation and immunosuppressive potential. The potential of CD271-mesenchymal stromal cells to improve the engraftment of CD133+ hematopoietic stem cells at co-transplantation was evaluated in immunodeficient NOD/SCID-IL2Rγnull mice. Results In vitro studies demonstrated that CD271-mesenchymal stromal cells differentiate along adipogenic, osteogenic and chondrogenic lineages (trilineage potential), produce significantly higher levels of cytokines than plastic adherence-mesenchymal stromal cells, and significantly inhibit the proliferation of allogeneic T-lymphocytes in mixed lymphocyte reaction assays. Elevated levels of prostaglandin E2, but not nitric monoxide, mediated the majority of this immunosuppressive effect. In vivo studies showed that CD271-mesenchymal stromal cells promoted significantly greater lymphoid engraftment than did plastic adherence-mesenchymal stromal cells when co-transplanted with CD133+ hematopoietic stem cells at a ratio of 8:1 in immunodeficient NOD/SCID-IL2Rγnull mice. They induced a 10.4-fold increase in the number of T cells, a 2.5-fold increase in the number of NK cells, and a 3.6-fold increase in the number of B cells, indicating a major qualitative difference

  14. 40 CFR 80.271 - How can a small refiner obtain an adjustment of its 2004-2007 per-gallon cap standard?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... adjustment of its 2004-2007 per-gallon cap standard? 80.271 Section 80.271 Protection of Environment...-2007 per-gallon cap standard? (a) EPA may in its discretion adjust the small refiner per-gallon cap... that the per-gallon cap creates; (2) The refiner's proposed adjusted per-gallon cap standard and the...

  15. The central star candidate of the planetary nebula Sh2-71: photometric and spectroscopic variability

    NASA Astrophysics Data System (ADS)

    Močnik, T.; Lloyd, M.; Pollacco, D.; Street, R. A.

    2015-07-01

    We present the analysis of several newly obtained and archived photometric and spectroscopic data sets of the intriguing and yet poorly understood 13.5 mag central star candidate of the bipolar planetary nebula Sh2-71. Photometric observations confirmed the previously determined quasi-sinusoidal light curve with a period of 68 d and also indicated periodic sharp brightness dips, possibly eclipses, with a period of 17.2 d. In addition, the comparison between U and V light curves revealed that the 68 d brightness variations are accompanied by a variable reddening effect of ΔE(U - V) = 0.38. Spectroscopic data sets demonstrated pronounced variations in spectral profiles of Balmer, helium and singly ionized metal lines and indicated that these variations occur on a time-scale of a few days. The most accurate verification to date revealed that spectral variability is not correlated with the 68 d brightness variations. The mean radial velocity of the observed star was measured to be ˜26 km s-1 with an amplitude of ±40 km s-1. The spectral type was determined to be B8V through spectral comparison with synthetic and standard spectra. The newly proposed model for the central star candidate is a Be binary with a misaligned precessing disc.

  16. Dissection of an inverted X(p21.3q27.1) chromosome associated with mental retardation.

    PubMed

    Leprêtre, F; Delannoy, V; Froguel, P; Vasseur, F; Montpellier, C

    2003-01-01

    In a 6 year old boy referred for mental retardation, fragile X syndrome was ruled out by cytogenetic and molecular analyses. Cytogenetic investigations revealed an inverted X chromosome (p21.3q27.1). A similar chromosomal rearrangement was detected in his mildly mentally retarded mother. Fluorescence in situ hybridization (FISH), using a panel of ordered YAC clones, allowed the identification of YACs spanning both the Xp21.3 and Xq27.1 breakpoints, where many non-specific mental retardation loci have been reported so far. Further investigations by FISH showed that the IL1RAPL1 gene at Xp21.3 was disrupted by the X chromosome inversion and therefore its inactivation may be related to the mental retardation observed in our patients.

  17. Characterization of endo-β-mannanase from Enterobacter ludwigii MY271 and application in pulp industry.

    PubMed

    Yang, Miao; Cai, Jun; Wang, Changgao; Du, Xin; Lin, Jianguo

    2017-01-01

    β-Mannanases are the second most important enzymes for the hydrolysis of hemicelluloses. An endo-β-mannanase from Enterobacter ludwigii MY271 was purified at 11.7 ± 0.2-fold to homogeneity with a final recovery of 15.2 ± 0.2 %. Using purified β-mannanase protein and SDS-PAGE, the molecular mass was found to be 43.16 kDa. The optimal pH and temperature of the enzyme was found to be 7.0 and 55 °C, respectively. The β-mannanase activity was stable over a broad pH range of pH 2.0-10.0. In addition, the purified enzyme was highly activated by several metal ions and chemical reagents, such as Mg(2+), L-cysteine, glutathione (GSH) and β-mercaptoethanol. Whereas the enzyme was strongly inhibited by Hg(2+), Cu(2+), N-bromosuccinimide (NBS), 1-ethyl-3-(3-dimethyl-amino-propyl)-carbodiimide (EDC), phenylmethanesulfonyl fluoride (PMSF), and sodium dodecyl sulfate (SDS). The β-mannanase was highly active towards glucomannan, and showed endo-activity by producing a mixture of oligosaccharides. Moreover, the enzyme displayed a classical endo-type mode on mannooligosaccharides. The β-mannanase coupled with xylanase significantly improved the brightness of kraft pulp, whereas it has no remarkable effect on the tensile strength of the pulp. Our functional studies of the purified β-mannanase indicate that the enzyme is beneficial to industrial applications, in particular, biotechnological processes, such as food, feed and pulp industry.

  18. High abundance of CD271+ multipotential stromal cells (MSCs) in intramedullary cavities of long bones

    PubMed Central

    Cox, George; Boxall, Sally A.; Giannoudis, Peter V.; Buckley, Conor T.; Roshdy, Tarek; Churchman, Sarah M.; McGonagle, Dennis; Jones, Elena

    2012-01-01

    Aspiration of iliac crest bone marrow (ICBM) remains the most frequent technique used in harvesting multipotential stromal cells (MSCs) for bone regeneration. Although this tissue type is easily accessed by a surgeon, it has a low frequency of MSCs, which is significant given the high cell numbers required for bone regeneration strategies. Lipoaspirates possess higher MSC frequencies, albeit cells with a differentiation profile less suited to orthopaedic interventions. Intra-medullary cavities of long bones have previously been shown to harbour MSCs in animals, however evaluation of their frequency, differentiation capacity and phenotype in humans had not previously been performed. Long bone fatty bone marrow (LBFBM) was collected prior to harvesting bone graft. Basic cellular compositions of donor-matched LBFBM and ICBM aspirates, including the numbers of CD34+ hematopoietic stem cells and CD31+ endothelial cells, were similar. MSCs were enumerated using colony-forming-unit-fibroblast assays and flow cytometry for the presence of a resident LBFBM CD45−/low CD271+ MSC population and revealed a trend for higher MSC numbers (average 5 fold, n = 6) per millilitre of LBFBM compared to donor-matched ICBM. Functional characteristics of resident MSCs, including their growth rates, differentiation potentials and surface phenotypes (CD73+CD105+CD90+) before and after culture-amplification, were similar. Enhanced numbers of MSCs could be recovered following brief enzymatic treatment of solid fragments of LBFBM. Our findings therefore reveal that the intramedullary cavity of the human femur is a depot of MSCs, which, although closely associated with fat, have a differentiation profile equivalent to ICBM. This anatomical site is frequently accessed by the orthopaedic/trauma surgeon and aspiration of the intramedullary cavity represents a ‘low-tech’ method of harvesting potentially large numbers of MSCs for regenerative therapies and research. This article is part of a

  19. Yield optimisation and molecular characterisation of uncultured CD271+ mesenchymal stem cells in the Reamer Irrigator Aspirator waste bag.

    PubMed

    Churchman, Sarah M; Kouroupis, Dimitrios; Boxall, Sally A; Roshdy, Tarek; Tan, Hiang B; McGonagle, Dennis; Giannoudis, Peter V; Jones, Elena A

    2013-12-13

    Bone reconstruction requires the use of autografts from patients' iliac crest (IC); for large-volume defects bone void fillers and autologous mesenchymal stem cells (MSCs) are often added. The Reamer/Irrigator/Aspirator (RIA) device provides the means of harvesting large amounts of autograft and additionally yields a waste bag containing MSCs, which is currently discarded. The aim of this study was to enumerate and characterise native MSCs from RIA waste bag and compare them to 'gold-standard' donor-matched MSCs from IC bone marrow (BM). IC-BM from age matched trauma patients was used as control. In RIA waste bags the median MSC yield established using a colony-forming fibroblast assay was 314333 (range 5 x 104-1.4 x 106), equivalent to approximately one litre of IC-BM aspirate. CD271+ cells were present at high levels in RIA waste bags, had MSC surface phenotype (CD90+CD73+CD105+CD34>sup>-CD61-CD19-CD31-CD33-) and expressed genes associated with multipotentiality, osteogenesis, adipogenesis and angiogenic support. RIA- CD271+ MSCs were transcriptionally similar to donor-matched IC-CD271+ MSCs (76 % transcripts); with the majority of bone-related and Wnt pathway molecules being expressed at comparable levels. Lower-level expression of MCAM/CD146 and 5/13 hypoxia-related molecules was found in RIA-CD271+ MSCs, potentially reflecting their native residence in a more hypoxic environment of the endosteum and bone cortex. These data suggest that long bones contain very large numbers of MSCs, transcriptionally-similar to IC-BM MSCs; they can be procured by reaming using the RIA device and used, following concentration, as autologous and potentially allogeneic bone repair therapy.

  20. Defective Ti2Nb10O27.1: an advanced anode material for lithium-ion batteries

    PubMed Central

    Lin, Chunfu; Yu, Shu; Zhao, Hua; Wu, Shunqing; Wang, Guizhen; Yu, Lei; Li, Yanfang; Zhu, Zi-Zhong; Li, Jianbao; Lin, Shiwei

    2015-01-01

    To explore anode materials with large capacities and high rate performances for the lithium-ion batteries of electric vehicles, defective Ti2Nb10O27.1 has been prepared through a facile solid-state reaction in argon. X-ray diffractions combined with Rietveld refinements indicate that Ti2Nb10O27.1 has the same crystal structure with stoichiometric Ti2Nb10O29 (Wadsley-Roth shear structure with A2/m space group) but larger lattice parameters and 6.6% O2– vacancies (vs. all O2– ions). The electronic conductivity and Li+ion diffusion coefficient of Ti2Nb10O27.1 are at least six orders of magnitude and ~2.5 times larger than those of Ti2Nb10O29, respectively. First-principles calculations reveal that the significantly enhanced electronic conductivity is attributed to the formation of impurity bands in Ti2Nb10O29–x and its conductor characteristic. As a result of the improvements in the electronic and ionic conductivities, Ti2Nb10O27.1 exhibits not only a large initial discharge capacity of 329 mAh g–1 and charge capacity of 286 mAh g–1 at 0.1 C but also an outstanding rate performance and cyclability. At 5 C, its charge capacity remains 180 mAh g–1 with large capacity retention of 91.0% after 100 cycles, whereas those of Ti2Nb10O29 are only 90 mAh g–1 and 74.7%. PMID:26632883