KSC-04PD-2404

NASA Technical Reports Server (NTRS)

2004-01-01

KENNEDY SPACE CENTER, FLA. On Launch Pad 17-B, Cape Canaveral Air Force Station, Fla., a second Solid Rocket Booster (SRB) is raised off a transporter to be lifted up the mobile service tower. It will be attached to the Boeing Delta II launch vehicle for launch of the Deep Impact spacecraft. A NASA Discovery mission, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth, and reveal the secrets of its interior. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will collect pictures and data of how the crater forms, measuring the craters depth and diameter, as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact project management is handled by the Jet Propulsion Laboratory in Pasadena, Calif. The spacecraft is scheduled to launch Dec. 30, 2004.

KSC-05PD-0075

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. The Deep Impact spacecraft waits inside the mobile service tower on Launch Pad 17-B, Cape Canaveral Air force Station, Fla., for fairing installation. The fairing is a molded structure that fits flush with the outside surface of the Delta II upper stage booster and forms an aerodynamically smooth nosecone, protecting the spacecraft during launch and ascent. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-04PD-2699

NASA Technical Reports Server (NTRS)

2004-01-01

KENNEDY SPACE CENTER, FLA. At Astrotech Space Operations in Titusville, Fla., the Deep Impact spacecraft is mated to the Boeing Delta II third stage. When the spacecraft and third stage are mated, they will be moved to Launch Pad 17-B at Cape Canaveral Air Force Station, Fla. There they will be mated to the Delta II rocket and the fairing installed around them for protection during launch and ascent. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-05PD-0079

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. Inside the mobile service tower on Launch Pad 17-B, Cape Canaveral Air force Station, Fla., the partly enclosed Deep Impact spacecraft (background) waits while the second half of the fairing (foreground left) moves toward it. The fairing is a molded structure that fits flush with the outside surface of the Delta II upper stage booster and forms an aerodynamically smooth nosecone, protecting the spacecraft during launch and ascent. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-05PD-0076

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. Inside the mobile service tower on Launch Pad 17-B, Cape Canaveral Air force Station, Fla., the first half of the fairing is moved toward the Deep Impact spacecraft for installation. The fairing is a molded structure that fits flush with the outside surface of the Delta II upper stage booster and forms an aerodynamically smooth nosecone, protecting the spacecraft during launch and ascent. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-05PD-0078

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. Inside the mobile service tower on Launch Pad 17-B, Cape Canaveral Air force Station, Fla., the first half of the fairing is moved into place around the Deep Impact spacecraft. The fairing is a molded structure that fits flush with the outside surface of the Delta II upper stage booster and forms an aerodynamically smooth nosecone, protecting the spacecraft during launch and ascent. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-04PD-2693

NASA Technical Reports Server (NTRS)

2004-01-01

KENNEDY SPACE CENTER, FLA. Boeing technicians at Astrotech Space Operations in Titusville, Fla., prepare the third stage of a Delta II rocket for mating with the Deep Impact spacecraft. When the spacecraft and third stage are mated, they will be moved to Launch Pad 17-B at Cape Canaveral Air Force Station, Fla. There they will be mated to the Delta II rocket and the fairing installed around them for protection during launch and ascent. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-05PD-0074

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. The Deep Impact spacecraft waits inside the mobile service tower on Launch Pad 17-B, Cape Canaveral Air force Station, Fla., for fairing installation. The fairing is a molded structure that fits flush with the outside surface of the Delta II upper stage booster and forms an aerodynamically smooth nosecone, protecting the spacecraft during launch and ascent. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-05PD-0077

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. Inside the mobile service tower on Launch Pad 17-B, Cape Canaveral Air force Station, Fla., the first half of the fairing is moved into place around the Deep Impact spacecraft. The fairing is a molded structure that fits flush with the outside surface of the Delta II upper stage booster and forms an aerodynamically smooth nosecone, protecting the spacecraft during launch and ascent. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-05PD-0073

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. The Deep Impact spacecraft waits inside the mobile service tower on Launch Pad 17-B, Cape Canaveral Air force Station, Fla., for fairing installation. The fairing is a molded structure that fits flush with the outside surface of the Delta II upper stage booster and forms an aerodynamically smooth nosecone, protecting the spacecraft during launch and ascent. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-05PD-0080

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. Inside the mobile service tower on Launch Pad 17-B, Cape Canaveral Air force Station, Fla., workers attach the two halves of the fairing around the Deep Impact spacecraft. The fairing is a molded structure that fits flush with the outside surface of the Delta II upper stage booster and forms an aerodynamically smooth nosecone, protecting the spacecraft during launch and ascent. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

Blinded by the Jets

NASA Image and Video Library

2005-07-04

This image shows the view from NASA Deep Impact probe 30 minutes before it was pummeled by comet Tempel 1. The picture brightness has been enhanced to show the jets of dust streaming away from the comet.

KSC-04PD-2413

NASA Technical Reports Server (NTRS)

2004-01-01

KENNEDY SPACE CENTER, FLA. On Launch Pad 17-B, Cape Canaveral Air Force Station, Fla., a crane begins lifting the third in a set of three Solid Rocket Boosters (SRBs). The SRBs will be hoisted up the mobile service tower and join three others already mated to the Boeing Delta II rocket that will launch the Deep Impact spacecraft. A NASA Discovery mission, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth, and reveal the secrets of its interior. After releasing an impactor on a course to hit the comets sunlit side, Deep Impacts flyby spacecraft will collect pictures and data of how the crater forms, measure the craters depth and diameter, as well as the composition of the interior of the crater and any material thrown out, and determine the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network.

KSC-04PD-2664

NASA Technical Reports Server (NTRS)

2004-01-01

KENNEDY SPACE CENTER, FLA. This view from inside the mobile service tower on Launch Pad 17-B, Cape Canaveral Air Force Station, shows the Boeing Delta II second stage as it reaches the top. The component will be reattached to the interstage adapter on the Delta II. The rocket is the launch vehicle for the Deep Impact spacecraft, scheduled for liftoff no earlier than Jan. 12. A NASA Discovery mission, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth, and reveal the secrets of its interior. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will collect pictures and data of how the crater forms, measuring the craters depth and diameter, as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network.

KSC-04PD-2662

NASA Technical Reports Server (NTRS)

2004-01-01

KENNEDY SPACE CENTER, FLA. At Launch Pad 17-B, Cape Canaveral Air Force Station, the Boeing Delta II second stage reaches the top of the mobile service tower. The component will be reattached to the interstage adapter on the Delta II. The rocket is the launch vehicle for the Deep Impact spacecraft, scheduled for liftoff no earlier than Jan. 12. A NASA Discovery mission, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth, and reveal the secrets of its interior. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will collect pictures and data of how the crater forms, measuring the craters depth and diameter, as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network.

KSC-04PD-2663

NASA Technical Reports Server (NTRS)

2004-01-01

KENNEDY SPACE CENTER, FLA. This view from inside the mobile service tower on Launch Pad 17-B, Cape Canaveral Air Force Station, shows the Boeing Delta II second stage as it reaches the top. The component will be reattached to the interstage adapter on the Delta II. The rocket is the launch vehicle for the Deep Impact spacecraft, scheduled for liftoff no earlier than Jan. 12. A NASA Discovery mission, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth, and reveal the secrets of its interior. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will collect pictures and data of how the crater forms, measuring the craters depth and diameter, as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network.

KSC-04PD-2697

NASA Technical Reports Server (NTRS)

2004-01-01

KENNEDY SPACE CENTER, FLA. At Astrotech Space Operations in Titusville, Fla., Boeing technicians oversee the final movement of the Deep Impact spacecraft being lowered onto the Delta II third stage for mating. When the spacecraft and third stage are mated, they will be moved to Launch Pad 17-B at Cape Canaveral Air Force Station, Fla. There they will be mated to the Delta II rocket and the fairing installed around them for protection during launch and ascent. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-04PD-2698

NASA Technical Reports Server (NTRS)

2004-01-01

KENNEDY SPACE CENTER, FLA. At Astrotech Space Operations in Titusville, Fla., Boeing technicians oversee the final movement of the Deep Impact spacecraft being lowered onto the Delta II third stage for mating. When the spacecraft and third stage are mated, they will be moved to Launch Pad 17-B at Cape Canaveral Air Force Station, Fla. There they will be mated to the Delta II rocket and the fairing installed around them for protection during launch and ascent. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-05PD-0013

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. The Deep Impact spacecraft is lifted from its transporter into the mobile service tower on Launch Pad 17-B at Cape Canaveral Air Force Station, Fla. the spacecraft will be attached to the second stage of the Boeing Delta II rocket. Next the fairing will be installed around the spacecraft. The fairing is a molded structure that fits flush with the outside surface of the Delta II upper stage booster and forms an aerodynamically smooth joint, protecting the spacecraft during launch and ascent. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-05PD-0010

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. At Astrotech Space Operations in Titusville, Fla., the Deep Impact spacecraft is secure in the canister for its move to Launch Pad 17-B on Cape Canaveral Air Force Station, Fla. Then, in the mobile service tower, the fairing will be installed around the spacecraft. The fairing is a molded structure that fits flush with the outside surface of the Delta II upper stage booster and forms an aerodynamically smooth joint, protecting the spacecraft during launch. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-04PD-2696

NASA Technical Reports Server (NTRS)

2004-01-01

KENNEDY SPACE CENTER, FLA. At Astrotech Space Operations in Titusville, Fla., Boeing technicians watch as an overhead crane lowers the Deep Impact spacecraft onto the Delta II third stage for mating. When the spacecraft and third stage are mated, they will be moved to Launch Pad 17-B at Cape Canaveral Air Force Station, Fla. There they will be mated to the Delta II rocket and the fairing installed around them for protection during launch and ascent. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3- foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-05PD-0012

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. The Deep Impact spacecraft arrives before dawn at the mobile service tower on Launch Pad 17-B at Cape Canaveral Air Force Station, Fla. The spacecraft will be attached to the second stage of the Boeing Delta II rocket. Next the fairing will be installed around the spacecraft. The fairing is a molded structure that fits flush with the outside surface of the Delta II upper stage booster and forms an aerodynamically smooth joint, protecting the spacecraft during launch and ascent. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-05PD-0017

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. In the mobile service tower on Launch Pad 17-B at Cape Canaveral Air Force Station, Fla., workers stand by as the canister is lifted away from the Deep Impact spacecraft. Next the fairing will be installed around the spacecraft. The fairing is a molded structure that fits flush with the outside surface of the Delta II upper stage booster and forms an aerodynamically smooth joint, protecting the spacecraft during launch and ascent. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-04PD-2695

NASA Technical Reports Server (NTRS)

2004-01-01

KENNEDY SPACE CENTER, FLA. At Astrotech Space Operations in Titusville, Fla., Boeing technicians watch as an overhead crane lifts the Deep Impact spacecraft, which is being moved for mating to the Delta II third stage. When the spacecraft and third stage are mated, they will be moved to Launch Pad 17-B at Cape Canaveral Air Force Station, Fla. There they will be mated to the Delta II rocket and the fairing installed around them for protection during launch and ascent. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-05PD-0018

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. In the mobile service tower on Launch Pad 17-B at Cape Canaveral Air Force Station, Fla., workers watch as the protective cover surrounding the Deep Impact spacecraft is lifted away. Next the fairing will be installed around the spacecraft. The fairing is a molded structure that fits flush with the outside surface of the Delta II upper stage booster and forms an aerodynamically smooth joint, protecting the spacecraft during launch and ascent. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-04PD-2694

NASA Technical Reports Server (NTRS)

2004-01-01

KENNEDY SPACE CENTER, FLA. At Astrotech Space Operations in Titusville, Fla., Boeing technicians attach a crane to the Deep Impact spacecraft in order to move it to the Delta II third stage at left for mating. When the spacecraft and third stage are mated, they will be moved to Launch Pad 17-B at Cape Canaveral Air Force Station, Fla. There they will be mated to the Delta II rocket and the fairing installed around them for protection during launch and ascent. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-05PD-0015

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. In the mobile service tower on Launch Pad 17-B at Cape Canaveral Air Force Station, Fla., workers begin lowering the Deep Impact spacecraft toward the second stage of the Boeing Delta II launch vehicle below for mating. Next the fairing will be installed around the spacecraft. The fairing is a molded structure that fits flush with the outside surface of the Delta II upper stage booster and forms an aerodynamically smooth joint, protecting the spacecraft during launch and ascent. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-05PD-0016

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. In the mobile service tower on Launch Pad 17-B at Cape Canaveral Air Force Station, Fla., workers attach the third stage motor, connected to the Deep Impact spacecraft, to the spin table on the second stage of the Boeing Delta II launch vehicle below. Next the fairing will be installed around the spacecraft. The fairing is a molded structure that fits flush with the outside surface of the Delta II upper stage booster and forms an aerodynamically smooth joint, protecting the spacecraft during launch and ascent. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-05PD-0014

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. The Deep Impact spacecraft is lifted into the top of the mobile service tower on Launch Pad 17-B at Cape Canaveral Air Force Station, Fla. the spacecraft will be attached to the second stage of the Boeing Delta II rocket. Next the fairing will be installed around the spacecraft. The fairing is a molded structure that fits flush with the outside surface of the Delta II upper stage booster and forms an aerodynamically smooth joint, protecting the spacecraft during launch and ascent. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

EPOXI Trip to Meet Comet Hartley 2

NASA Image and Video Library

2010-10-26

This frame from a movie begins with the launch of NASA Deep Impact on Jan. 12, 2005, from Cape Canaveral, Fla. On July 4, 2005, the mission released a probe into Comet Tempel 1, revealing its pristine, inner material.

KSC-05PD-0116

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. At Ball Aerospace in Boulder, Colo., the impactor on the Deep Impact spacecraft is tested. Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth, and reveal the secrets of its interior. After releasing a 3- by 3-foot projectile (impactor) to crash onto the surface, Deep Impacts flyby spacecraft will collect pictures and data of how the crater forms, measuring the craters depth and diameter, as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. The impactor will separate from the flyby spacecraft 24 hours before it impacts the surface of Tempel 1's nucleus. The impactor delivers 19 Gigajoules (that's 4.8 tons of TNT) of kinetic energy to excavate the crater. This kinetic energy is generated by the combination of the mass of the impactor and its velocity when it impacts. To accomplish this feat, the impactor uses a high-precision star tracker, the Impactor Target Sensor (ITS), and Auto-Navigation algorithms developed by Jet Propulsion Laboratory to guide it to the target. Deep Impact is a NASA Discovery mission. Launch of Deep Impact is scheduled for Jan. 12 from Launch Pad 17-B, Cape Canaveral Air Force Station, Fla.

KSC-04PD-2533

NASA Technical Reports Server (NTRS)

2004-01-01

KENNEDY SPACE CENTER, FLA. Ball Aerospace technicians at Astrotech in Titusville, Fla., begin lifting the high-gain communications antenna to attach it to an overhead crane. The antenna will be installed on the Deep Impact spacecraft. A NASA Discovery mission, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth, and reveal the secrets of its interior. During the encounter phase, the high-gain antenna transmits near-real- time images of the impact back to Earth. The spacecraft is scheduled to launch Jan. 8 aboard a Boeing Delta II rocket from Launch Complex 17-B at Cape Canaveral Air Force Station, Fla.

KSC-04PD-2180

NASA Technical Reports Server (NTRS)

2004-01-01

KENNEDY SPACE CENTER, FLA. At Astrotech Space Operations in Titusville, Fla., Joe Galamback mounts a bracket on a solar panel on the Deep Impact spacecraft. Galamback is a lead mechanic technician with Ball Aerospace and Technologies Corp. in Boulder, Colo. The spacecraft is undergoing verification testing after its long road trip from Colorado.A NASA Discovery mission, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth, and reveal the secrets of its interior. After releasing a 3- by 3- foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will collect pictures and data of how the crater forms, measuring the craters depth and diameter, as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. The spacecraft is scheduled to launch Dec. 30, 2004, aboard a Boeing Delta II rocket from Launch Complex 17 at Cape Canaveral Air Force Station, Fla.

KSC-05PD-0001

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. The Deep Impact spacecraft waits at Astrotech Space Operations in Titusville, Fla., for placement of a protective cover before the canister is installed around it. Once the spacecraft is completely covered, it will be transferred to Launch Pad 17-B on Cape Canaveral Air Force Station, Fla. Then, in the mobile service tower, the fairing will be installed around the spacecraft. The fairing is a molded structure that fits flush with the outside surface of the Delta II upper stage booster and forms an aerodynamically smooth joint, protecting the spacecraft during launch. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

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NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. At Astrotech Space Operations in Titusville, Fla., Boeing technicians place the lower segments of a protective canister around the Deep Impact spacecraft. Once the spacecraft is completely covered, it will be transferred to Launch Pad 17-B on Cape Canaveral Air Force Station, Fla. Then, in the mobile service tower, the fairing will be installed around the spacecraft. The fairing is a molded structure that fits flush with the outside surface of the Delta II upper stage booster and forms an aerodynamically smooth joint, protecting the spacecraft during launch. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-05PD-0007

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. At Astrotech Space Operations in Titusville, Fla., technicians lower the upper canister toward the Deep Impact spacecraft. It will be attached to the lower segments already surrounding the spacecraft. Once the spacecraft is completely covered, it will be transferred to Launch Pad 17-B on Cape Canaveral Air Force Station, Fla. Then, in the mobile service tower, the fairing will be installed around the spacecraft. The fairing is a molded structure that fits flush with the outside surface of the Delta II upper stage booster and forms an aerodynamically smooth joint, protecting the spacecraft during launch. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-05PD-0005

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. At Astrotech Space Operations in Titusville, Fla., Boeing technicians roll the Deep Impact spacecraft into another area where the upper canister can be lowered around it. Once the spacecraft is completely covered, it will be transferred to Launch Pad 17-B on Cape Canaveral Air Force Station, Fla. Then, in the mobile service tower, the fairing will be installed around the spacecraft. The fairing is a molded structure that fits flush with the outside surface of the Delta II upper stage booster and forms an aerodynamically smooth joint, protecting the spacecraft during launch. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-05PD-0002

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. At Astrotech Space Operations in Titusville, Fla., a protective cover is being lowered over the Deep Impact spacecraft to protect it before the canister is installed around it. Once the spacecraft is completely covered, it will be transferred to Launch Pad 17-B on Cape Canaveral Air Force Station, Fla. Then, in the mobile service tower, the fairing will be installed around the spacecraft. The fairing is a molded structure that fits flush with the outside surface of the Delta II upper stage booster and forms an aerodynamically smooth joint, protecting the spacecraft during launch. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-05PD-0011

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. The Deep Impact spacecraft leaves Astrotech Space Operations in Titusville, Fla., in the pre-dawn hours on a journey to Launch Pad 17-B at Cape Canaveral Air Force Station, Fla. There the spacecraft will be attached to the second stage of the Boeing Delta II rocket. Next the fairing will be installed around the spacecraft. The fairing is a molded structure that fits flush with the outside surface of the Delta II upper stage booster and forms an aerodynamically smooth joint, protecting the spacecraft during launch and ascent. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-05PD-0003

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. At Astrotech Space Operations in Titusville, Fla., Boeing technicians lower a protective cover over the Deep Impact spacecraft to protect it before the canister is installed around it. Once the spacecraft is completely covered, it will be transferred to Launch Pad 17-B on Cape Canaveral Air Force Station, Fla. Then, in the mobile service tower, the fairing will be installed around the spacecraft. The fairing is a molded structure that fits flush with the outside surface of the Delta II upper stage booster and forms an aerodynamically smooth joint, protecting the spacecraft during launch. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3- foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-05PD-0006

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. At Astrotech Space Operations in Titusville, Fla., technicians install a crane onto the upper canister before lifting it to install around the Deep Impact spacecraft. Once the spacecraft is completely covered, it will be transferred to Launch Pad 17-B on Cape Canaveral Air Force Station, Fla. Then, in the mobile service tower, the fairing will be installed around the spacecraft. The fairing is a molded structure that fits flush with the outside surface of the Delta II upper stage booster and forms an aerodynamically smooth joint, protecting the spacecraft during launch. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-05PD-0009

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. At Astrotech Space Operations in Titusville, Fla., Boeing technicians attach the upper canister with the lower segments surrounding the Deep Impact spacecraft. Once the spacecraft is completely covered, it will be transferred to Launch Pad 17-B on Cape Canaveral Air Force Station, Fla. Then, in the mobile service tower, the fairing will be installed around the spacecraft. The fairing is a molded structure that fits flush with the outside surface of the Delta II upper stage booster and forms an aerodynamically smooth joint, protecting the spacecraft during launch. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-05PD-0008

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. At Astrotech Space Operations in Titusville, Fla., technicians lower the upper canister toward the Deep Impact spacecraft. It will be attached to the lower segments already surrounding the spacecraft. Once the spacecraft is completely covered, it will be transferred to Launch Pad 17-B on Cape Canaveral Air Force Station, Fla. Then, in the mobile service tower, the fairing will be installed around the spacecraft. The fairing is a molded structure that fits flush with the outside surface of the Delta II upper stage booster and forms an aerodynamically smooth joint, protecting the spacecraft during launch. Scheduled for liftoff Jan. 12, Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile to crash onto the surface, Deep Impacts flyby spacecraft will reveal the secrets of its interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-05PD-0114

NASA Technical Reports Server (NTRS)

2005-01-01

the Fischer Assembly building at Ball Aerospace in Boulder, Colo. Deep Impact will probe beneath the surface of Comet Tempel 1 on July 4, 2005, when the comet is 83 million miles from Earth, and reveal the secrets of its interior. After releasing a 3- by 3- foot projectile (impactor) to crash onto the surface, Deep Impacts flyby spacecraft will collect pictures and data of how the crater forms, measuring the craters depth and diameter, as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. The impactor will separate from the flyby spacecraft 24 hours before it impacts the surface of Tempel 1's nucleus. The impactor delivers 19 Gigajoules (that's 4.8 tons of TNT) of kinetic energy to excavate the crater. This kinetic energy is generated by the combination of the mass of the impactor and its velocity when it impacts. To accomplish this feat, the impactor uses a high-precision star tracker, the Impactor Target Sensor (ITS), and Auto-Navigation algorithms developed by Jet Propulsion Laboratory to guide it to the target. Deep Impact is a NASA Discovery mission. Launch of Deep Impact is scheduled for Jan. 12 from Launch Pad 17-B, Cape Canaveral Air Force Station, Fla.

Neutrino-Nucleon Deep Inelastic Scattering in MINERvA

NASA Astrophysics Data System (ADS)

Norrick, Anne; Minerva Collaboration

2015-04-01

Neutrino-Nucleon Deep Inelastic Scattering (DIS) events provide a probe into the structure of the nucleus that cannot be accessed via charged lepton-nucleon interactions. The MINERvA experiment is stationed in the Neutrinos from the Main Injector (NuMI) beam line at Fermi National Accelerator Laboratory. The projected sensitivity of nuclear structure function analyses using MINERvA's suite of nuclear targets (C, CH, Fe and Pb) in the upgraded 6 GeV neutrino energy NuMI beam will be explored, and their impact discussed.

Deep Impact Spacecraft Collides With Comet Tempel 1-Video

NASA Technical Reports Server (NTRS)

2005-01-01

After 172 days and 268 million miles of deep space travel, the NASA Deep Impact spacecraft successfully reached out and touched comet Tempel 1. The collision between the coffee table-sized space probe and city-sized comet occurred July 4, 2005 at 12:52 a.m. CDT. The objects met at 23,000 miles per hour. The heat produced by the impact was at least several thousand degrees Kelvin and at that extreme temperature, just about any material begins to glow. This movie, made up of images taken by the medium resolution camera aboard the spacecraft, from May 1 to July 2, shows the Deep Impact approach to comet Tempel 1. The spacecraft detected 3 outbursts during this time period, on June 14th, June 22nd, and July 2nd. The movie ends during the final outburst. Mission scientists expect Deep Impact to provide answers to basic questions about the formation of the solar system. Principal investigator, Dr. Michael A'Hearn of the University of Maryland in College Park, is responsible for the mission, and project management is handled by the Jet Propulsion Laboratory in Pasadena, California. The program office at Marshall Space Flight Center MSFC) in Huntsville, Alabama, assisted the Science Mission Directorate at NASA Headquarters in Washington with program management, technology planning, systems assessment, flight assurance and public outreach. The spacecraft was built for NASA by Ball Aerospace & Technologies Corporation of Boulder, Colorado. (NASA/JPL-Caltech/UMD)

The impact of semantic impairment on word stem completion in Alzheimer's disease.

PubMed

Beauregard, M; Chertkow, H; Gold, D; Bergman, S

2001-01-01

Both the extent of semantic memory impairment and the level of processing attained during encoding might constitute critical factors in determining the amount of word-stem completion (WSC) priming encountered in Alzheimer's disease (AD) subjects. We investigated the impact of varying encoding level in AD and elderly normal subjects, using a set of stimuli ranked as "intact" or "degraded" in terms of each subject's semantic knowledge on probe questions. For both shallow and deep encoding conditions, overall priming in the two subject groups was equivalent. However, for the deep encoding condition, consisting of a semantic judgment task performed on each target word, the priming effect noted in AD subjects was significantly smaller for semantically degraded items than for semantically intact items. Results indicate that the degree of semantic impairment represents one important variable affecting the amount of WSC priming which results when deep encoding procedures are used at study.

A Cyber-Astronaut's Final Moves

NASA Technical Reports Server (NTRS)

2005-01-01

This image shows how Deep Impact's impactor targeted comet Tempel 1 as the spacecraft made its final approach in the early morning hours of July 4, Eastern time. The autonomous navigation system on the probe was designed to make as many as three impactor targeting maneuvers, identified as ITMs in this picture, to correct its course to the comet.

The upper left dot indicates where the probe would have passed the comet's nucleus if no maneuvers were performed. The dot below the nucleus shows where the probe would have flown past the comet if only the first maneuver was made. The leftmost dot on the nucleus marks the spot where the probe would have crunched the comet if only the first two maneuvers had been performed. The lower dot on the nucleus indicates the vicinity where, once the third maneuver was performed, the probe met its final reward and collided with the comet.

Profiling of barrier capacitance and spreading resistance using a transient linearly increasing voltage technique.

PubMed

Gaubas, E; Ceponis, T; Kusakovskij, J

2011-08-01

A technique for the combined measurement of barrier capacitance and spreading resistance profiles using a linearly increasing voltage pulse is presented. The technique is based on the measurement and analysis of current transients, due to the barrier and diffusion capacitance, and the spreading resistance, between a needle probe and sample. To control the impact of deep traps in the barrier capacitance, a steady state bias illumination with infrared light was employed. Measurements of the spreading resistance and barrier capacitance profiles using a stepwise positioned probe on cross sectioned silicon pin diodes and pnp structures are presented.

Deep Flaw Detection with Giant Magnetoresistive (GMR) Based Self-Nulling Probe

NASA Technical Reports Server (NTRS)

Wincheski, Buzz; Namkung, Min

2004-01-01

In this paper a design modification to the Very-Low Frequency GMR Based Self-Nulling Probe has been presented to enable improved signal to noise ratio for deeply buried flaws. The design change consists of incorporating a feedback coil in the center of the flux focusing lens. The use of the feedback coil enables cancellation of the leakage fields in the center of the probe and biasing of the GMR sensor to a location of high magnetic field sensitivity. The effect of the feedback on the probe output was examined, and experimental results for deep flaw detection were presented. The experimental results show that the modified probe is capable of clearly identifying flaws up to 1 cm deep in aluminum alloy structures.

Sunny Side of a Comet

NASA Technical Reports Server (NTRS)

2005-01-01

[figure removed for brevity, see original site] Figure 1: Temperature Map

This image composite shows comet Tempel 1 in visible (left) and infrared (right) light (figure 1). The infrared picture highlights the warm, or sunlit, side of the comet, where NASA's Deep Impact probe later hit. These data were acquired about six minutes before impact. The visible image was taken by the medium-resolution camera on the mission's flyby spacecraft, and the infrared data were acquired by the flyby craft's infrared spectrometer.

Direct Communication to Earth from Probes

NASA Technical Reports Server (NTRS)

Bolton, Scott J.; Folkner, William M.; Abraham, Douglas S.

2005-01-01

A viewgraph presentation on outer planetary probe communications to Earth is shown. The topics include: 1) Science Rational for Atmospheric Probes to the Outer Planets; 2) Controlling the Scientific Appetite; 3) Learning more about Jupiter before we send more probes; 4) Sample Microwave Scan From Juno; 5) Jupiter s Deep Interior; 6) The Square Kilometer Array (SKA): A Breakthrough for Radio Astronomy; 7) Deep Space Array-based Network (DSAN); 8) Probe Direct-to-Earth Data Rate Calculations; 9) Summary; and 10) Enabling Ideas.

Looking Back at a Job Well Done

NASA Technical Reports Server (NTRS)

2005-01-01

This image shows the view from Deep Impact's flyby spacecraft as it turned back to look at comet Tempel 1. Fifty minutes earlier, the spacecraft's probe was run over by the comet. That collision kicked up plumes of ejected material, seen here streaming away from the back side of the comet. This image was taken by the flyby craft's high-resolution camera.

Flexible deep brain neural probes based on a parylene tube structure

NASA Astrophysics Data System (ADS)

Zhao, Zhiguo; Kim, Eric; Luo, Hao; Zhang, Jinsheng; Xu, Yong

2018-01-01

Most microfabricated neural probes have limited shank length, which prevents them from reaching many deep brain structures. This paper reports deep brain neural probes with ultra-long penetrating shanks based on a simple but novel parylene tube structure. The mechanical strength of the parylene tube shank is temporarily enhanced during implantation by inserting a metal wire. The metal wire can be removed after implantation, making the implanted probe very flexible and thus minimizing the stress caused by micromotions of brain tissues. Optogenetic stimulation and chemical delivery capabilities can be potentially integrated by taking advantage of the tube structure. Single-shank prototypes with a shank length of 18.2 mm have been developed. The microfabrication process comprises of deep reactive ion etching (DRIE) of silicon, parylene conformal coating/refilling, and XeF2 isotropic silicon etching. In addition to bench-top insertion characterization, the functionality of developed probes has been preliminarily demonstrated by implanting into the amygdala of a rat and recording neural signals.

Study of alternative probe technologies

NASA Technical Reports Server (NTRS)

1977-01-01

A number of implied technologies for a deep probe mission was examined; i.e., one that would provide the capability to scientifically examine planetary atmospheres at the 1000 bar level. Conditions imposed by current Jupiter, Saturn, and Uranus atmospheric models were considered. The major thrust of the measurements was to determine lower atmosphere composition, even to trace constituents of one part per billion. Two types of instruments having the necessary accuracy to meet the science objectives were considered and integrated into a deep probe configuration. One deep probe option that resulted was identified as a Minimum Technology Development approach. The significant feature of this option is that only three technology developments are required to enable the mission, i.e., (1) science instrument development, (2) advanced data processing, and (3) external high pressure/thermal insulation. It is concluded that a probe designed for a Jupiter mission could, with minor changes, be used for a Saturn or Uranus mission.

A nonintrusive temperature measuring system for estimating deep body temperature in bed.

PubMed

Sim, S Y; Lee, W K; Baek, H J; Park, K S

2012-01-01

Deep body temperature is an important indicator that reflects human being's overall physiological states. Existing deep body temperature monitoring systems are too invasive to apply to awake patients for a long time. Therefore, we proposed a nonintrusive deep body temperature measuring system. To estimate deep body temperature nonintrusively, a dual-heat-flux probe and double-sensor probes were embedded in a neck pillow. When a patient uses the neck pillow to rest, the deep body temperature can be assessed using one of the thermometer probes embedded in the neck pillow. We could estimate deep body temperature in 3 different sleep positions. Also, to reduce the initial response time of dual-heat-flux thermometer which measures body temperature in supine position, we employed the curve-fitting method to one subject. And thereby, we could obtain the deep body temperature in a minute. This result shows the possibility that the system can be used as practical temperature monitoring system with appropriate curve-fitting model. In the next study, we would try to establish a general fitting model that can be applied to all of the subjects. In addition, we are planning to extract meaningful health information such as sleep structure analysis from deep body temperature data which are acquired from this system.

Theoretical simulation of the dual-heat-flux method in deep body temperature measurements.

PubMed

Huang, Ming; Chen, Wenxi

2010-01-01

Deep body temperature reveals individual physiological states, and is important in patient monitoring and chronobiological studies. An innovative dual-heat-flux method has been shown experimentally to be competitive with the conventional zero-heat-flow method in its performance, in terms of measurement accuracy and step response to changes in the deep temperature. We have utilized a finite element method to model and simulate the dynamic process of a dual-heat-flux probe in deep body temperature measurements to validate the fundamental principles of the dual-heat-flux method theoretically, and to acquire a detailed quantitative description of the thermal profile of the dual-heat-flux probe. The simulation results show that the estimated deep body temperature is influenced by the ambient temperature (linearly, at a maximum rate of 0.03 °C/°C) and the blood perfusion rate. The corresponding depth of the estimated temperature in the skin and subcutaneous tissue layer is consistent when using the dual-heat-flux probe. Insights in improving the performance of the dual-heat-flux method were discussed for further studies of dual-heat-flux probes, taking into account structural and geometric considerations.

Local coexistence of VO 2 phases revealed by deep data analysis

DOE PAGES

Strelcov, Evgheni; Ievlev, Anton; Tselev, Alexander; ...

2016-07-07

We report a synergistic approach of micro-Raman spectroscopic mapping and deep data analysis to study the distribution of crystallographic phases and ferroelastic domains in a defected Al-doped VO 2 microcrystal. Bayesian linear unmixing revealed an uneven distribution of the T phase, which is stabilized by the surface defects and uneven local doping that went undetectable by other classical analysis techniques such as PCA and SIMPLISMA. This work demonstrates the impact of information recovery via statistical analysis and full mapping in spectroscopic studies of vanadium dioxide systems, which is commonly substituted by averaging or single point-probing approaches, both of which suffermore » from information misinterpretation due to low resolving power.« less

A Survey of the Rapidly Emerging Field of Nanotechnology: Potential Applications for Scientific Instruments and Technologies for Atmospheric Entry Probes

NASA Technical Reports Server (NTRS)

Meyyappan, M.; Arnold, J. O.

2005-01-01

The field of Nanotechnology is well funded worldwide and innovations applicable to Solar System Exploration are emerging much more rapidly than thought possible just a few years ago. This presentation will survey recent innovations from nanotechnololgy with a focus on novel applications to atmospheric entry science and probe technology, in a fashion similar to that presented by Arnold and Venkatapathy at the previous workshop forum at Lisbon Portugal, October 6-9, 2003. Nanotechnology is a rapidly emerging field that builds systems, devices and materials from the bottom up, atom by atom, and in so doing provides them with novel and remarkable macro-scale performance. This technology has the potential to revolutionize space exploration by reducing mass and simultaneously increasing capability. Thermal, Radiation, Impact Protective Shields: Atmospheric probes and humans on long duration deep space missions involved in Solar System Exploration must safely endure 3 significant hazards: (i) atmospheric entry; (ii) radiation; and (iii) micrometeorite or debris impact. Nanostructured materials could be developed to address all three hazards with a single protective shield, which would involve much less mass than a traditional approach. The concept can be ready in time for incorporation into NASA s Crew Exploration Vehicle, and possible entry probes to fly on the Jupiter Icy Moons

The Mars Microprobe Mission: Advanced Micro-Avionics for Exploration Surface

NASA Astrophysics Data System (ADS)

Blue, Randel

2000-01-01

The Mars Microprobe Mission is the second spacecraft developed as part of the New Millennium Program deep space missions. The objective of the Microprobe Project is to demonstrate the applicability of key technologies for future planetary missions by developing two probes for deployment on Mars. The probes are designed with a single stage entry, descent, and landing system and impact the Martian surface at speeds of approximately 200 meters per second. The microprobes are composed of two main sections, a forebody section that penetrates to a depth below the Martian surface of 0.5 to 2 meters, and an aftbody section that remains on the surface. Each probe system consists of a number of advanced technology components developed specifically for this mission. These include a non-erosive aeroshell for entry into. the atmosphere, a set of low temperature batteries to supply probe power, an advanced microcontroller to execute the mission sequence, collect the science data, and react to possible system fault conditions, a telecommunications subsystem implemented on a set of custom integrated circuits, and instruments designed to provide science measurements from above and below the Martian surface. All of the electronic components have been designed and fabricated to withstand the severe impact shock environment and to operate correctly at predicted temperatures below -100 C.

Photoacoustic imaging probe for detecting lymph nodes and spreading of cancer at various depths

NASA Astrophysics Data System (ADS)

Lee, Yong-Jae; Jeong, Eun-Ju; Song, Hyun-Woo; Ahn, Chang-Geun; Noh, Hyung Wook; Sim, Joo Yong; Song, Dong Hoon; Jeon, Min Yong; Lee, Susung; Kim, Heewon; Zhang, Meihua; Kim, Bong Kyu

2017-09-01

We propose a compact and easy to use photoacoustic imaging (PAI) probe structure using a single strand of optical fiber and a beam combiner doubly reflecting acoustic waves for convenient detection of lymph nodes and cancers. Conventional PAI probes have difficulty detecting lymph nodes just beneath the skin or simultaneously investigating lymph nodes located in shallow as well as deep regions from skin without any supplementary material because the light and acoustic beams are intersecting obliquely in the probe. To overcome the limitations and improve their convenience, we propose a probe structure in which the illuminated light beam axis coincides with the axis of the ultrasound. The developed PAI probe was able to simultaneously achieve a wide range of images positioned from shallow to deep regions without the use of any supplementary material. Moreover, the proposed probe had low transmission losses for the light and acoustic beams. Therefore, the proposed PAI probe will be useful to easily detect lymph nodes and cancers in real clinical fields.

Lunar environment and design of China's first moon rover Yutu

NASA Astrophysics Data System (ADS)

Jianhui, Wu

China launched the Chang'e-3 lunar probe with the country's first moon rover aboard on Dec.14, marking a significant step toward deep space exploration.Lunar environment and environmental tests of typical lunar survyeors are discussed in this papaer.According to the needs of China's lunar exploration project,environmental impact of moon rovers and Yutu design ideas are studied.Through the research, temperature control device, micro-gravity environment design ,dust and other equipment devices used on Yutu all meet the mission requirements.

Development and Application of Wide Bandwidth Magneto-Resistive Sensor Based Eddy Current Probe

NASA Technical Reports Server (NTRS)

Wincheski, Russell A.; Simpson, John

2010-01-01

The integration of magneto-resistive sensors into eddy current probes can significantly expand the capabilities of conventional eddy current nondestructive evaluation techniques. The room temperature solid-state sensors have typical bandwidths in the megahertz range and resolutions of tens of microgauss. The low frequency sensitivity of magneto-resistive sensors has been capitalized upon in previous research to fabricate very low frequency eddy current sensors for deep flaw detection in multilayer conductors. In this work a modified probe design is presented to expand the capabilities of the device. The new probe design incorporates a dual induction source enabling operation from low frequency deep flaw detection to high frequency high resolution near surface material characterization. Applications of the probe for the detection of localized near surface conductivity anomalies are presented. Finite element modeling of the probe is shown to be in good agreement with experimental measurements.

RGD-conjugated two-photon absorbing near-IR emitting fluorescent probes for tumor vascular imaging (Conference Presentation)

NASA Astrophysics Data System (ADS)

Belfield, Kevin D.; Yue, Xiling; Morales, Alma R.; Githaiga, Grace W.; Woodward, Adam W.; Tang, Simon; Sawada, Junko; Komatsu, Masanobu; Liu, Xuan

2016-03-01

Observation of the activation and inhibition of angiogenesis processes is important in the progression of cancer. Application of targeting peptides, such as a small peptide that contains adjacent L-arginine (R), glycine (G) and L-aspartic acid (D) residues can afford high selectivity and deep penetration in vessel imaging. To facilitate deep tissue vasculature imaging, probes that can be excited via two-photon absorption (2PA) in the near-infrared (NIR) and subsequently emit in the NIR are essential. In this study, the enhancement of tissue image quality with RGD conjugates was investigated with new NIR-emitting pyranyl fluorophore derivatives in two-photon fluorescence microscopy. Linear and nonlinear photophysical properties of the new probes were comprehensively characterized; significantly the probes exhibited good 2PA over a broad spectral range from 700-1100 nm. Cell and tissue images were then acquired and examined, revealing deep penetration and high contrast with the new pyranyl RGD-conjugates up to 350 μm in tumor tissue.

KSC-05PD-0147

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. NASA Administrator Sean OKeefe (center) is presented with a Deep Impact hat in the Press Site Auditorium following his report to employees on the state of the Agency. He is accompanied on stage by Center Director Jim Kennedy (right). The update was broadcast live via NASA Television. O'Keefe focused on the achievements of 2004 and the goals set for 2005. His remarks emphasized the milestones met in NASA's Vision for Space Exploration, including the launch of the comet-chasing Deep Impact mission and the landing of the Huygens probe on Jupiters moon Titan, both occurring in the past two days, and the progress made in meeting the requirements to return the Space Shuttle to flight. OKeefes briefing included a dialogue with Associate Administrator of NASAs Office of Exploration Systems Craig Steidle and Center Director Jim Kennedy, live; and Manager of the Space Station Office Bill Gerstenmaier and Director of Advanced Planning and Jet Propulsion Laboratory Charles Elachi, via satellite.

Imaging deep skeletal muscle structure using a high-sensitivity ultrathin side-viewing optical coherence tomography needle probe

PubMed Central

Yang, Xiaojie; Lorenser, Dirk; McLaughlin, Robert A.; Kirk, Rodney W.; Edmond, Matthew; Simpson, M. Cather; Grounds, Miranda D.; Sampson, David D.

2013-01-01

We have developed an extremely miniaturized optical coherence tomography (OCT) needle probe (outer diameter 310 µm) with high sensitivity (108 dB) to enable minimally invasive imaging of cellular structure deep within skeletal muscle. Three-dimensional volumetric images were acquired from ex vivo mouse tissue, examining both healthy and pathological dystrophic muscle. Individual myofibers were visualized as striations in the images. Degradation of cellular structure in necrotic regions was seen as a loss of these striations. Tendon and connective tissue were also visualized. The observed structures were validated against co-registered hematoxylin and eosin (H&E) histology sections. These images of internal cellular structure of skeletal muscle acquired with an OCT needle probe demonstrate the potential of this technique to visualize structure at the microscopic level deep in biological tissue in situ. PMID:24466482

Noncontact diffuse correlation spectroscopy for noninvasive deep tissue blood flow measurement

NASA Astrophysics Data System (ADS)

Lin, Yu; He, Lian; Shang, Yu; Yu, Guoqiang

2012-01-01

A noncontact diffuse correlation spectroscopy (DCS) probe has been developed using two separated optical paths for the source and detector. This unique design avoids the interference between the source and detector and allows large source-detector separations for deep tissue blood flow measurements. The noncontact probe has been calibrated against a contact probe in a tissue-like phantom solution and human muscle tissues; flow changes concurrently measured by the two probes are highly correlated in both phantom (R2=0.89, p<10-5) and real-tissue (R2=0.77, p<10-5, n=9) tests. The noncontact DCS holds promise for measuring blood flow in vulnerable (e.g., pressure ulcer) and soft (e.g., breast) tissues without distorting tissue hemodynamic properties.

Neptune Polar Orbiter with Probes

NASA Technical Reports Server (NTRS)

Bienstock, Bernard; Atkinson, David; Baines, Kevin; Mahaffy, Paul; Steffes, Paul; Atreya, Sushil; Stern, Alan; Wright, Michael; Willenberg, Harvey; Smith, David;

Deep-Reaching Hydrodynamic Flow Confinement: Micrometer-Scale Liquid Localization for Open Substrates With Topographical Variations.

PubMed

Oskooei, Ali; Kaigala, Govind V

2017-06-01

We present a method for nonintrusive localization and reagent delivery on immersed biological samples with topographical variation on the order of hundreds of micrometers. Our technique, which we refer to as the deep-reaching hydrodynamic flow confinement (DR-HFC), is simple and passive: it relies on a deep-reaching hydrodynamic confinement delivered through a simple microfluidic probe design to perform localized microscale alterations on substrates as deep as 600 μm. Designed to scan centimeter-scale areas of biological substrates, our method passively prevents sample intrusion by maintaining a large gap between the probe and the substrate. The gap prevents collision of the probe and the substrate and reduces the shear stress experienced by the sample. We present two probe designs: linear and annular DR-HFC. Both designs comprise a reagent-injection aperture and aspiration apertures that serve to confine the reagent. We identify the design parameters affecting reagent localization and depth by DR-HFC and study their individual influence on the operation of DR-HFC numerically. Using DR-HFC, we demonstrate localized binding of antihuman immunoglobulin G (IgG) onto an activated substrate at various depths from 50 to 600 μm. DR-HFC provides a readily implementable approach for noninvasive processing of biological samples applicable to the next generation of diagnostic and bioanalytical devices.

Deep Space Chronicle: A Chronology of Deep Space and Planetary Probes 1958-2000

NASA Technical Reports Server (NTRS)

Siddiqi, Asif A.; Launius, Roger (Technical Monitor)

2002-01-01

This monograph contains brief descriptions of all robotic deep space missions attempted since the opening of the space age in 1957. The missions are listed strictly chronologically in order of launch date (not by planetary encounter).

X-Ray Probes of Cosmic Star-Formation History

NASA Technical Reports Server (NTRS)

Ghosh, Pranab; White, Nicholas E.

2001-01-01

In a previous paper we point out that the X-ray luminosity L(sub x) of a galaxy is driven by the evolution of its X-ray binary population and that the profile of L(sub x) with redshift can both serve as a diagnostic probe of the Star Formation Rate (SFR) profile and constrain evolutionary models for X-ray binaries. We update our previous work using a suite of more recently developed SFR profiles that span the currently plausible range. The first Chandra deep imaging results on L(sub x)-evolution are beginning to probe the SFR profile of bright spirals and the early results are consistent with predictions based on current SFR models. Using these new SFR profiles the resolution of the "birthrate problem" of lowmass X-ray binaries (LMXBs) and recycled, millisecond pulsars in terms of an evolving global SFR is more complete. We also discuss the possible impact of the variations in the SFR profile of individual galaxies.

Nuclear Thermal Rocket - Arc Jet Integrated System Model

NASA Technical Reports Server (NTRS)

Taylor, Brian D.; Emrich, William

2016-01-01

In the post-shuttle era, space exploration is moving into a new regime. Commercial space flight is in development and is planned to take on much of the low earth orbit space flight missions. With the development of a heavy lift launch vehicle, the Space Launch, System, NASA has become focused on deep space exploration. Exploration into deep space has traditionally been done with robotic probes. More ambitious missions such as manned missions to asteroids and Mars will require significant technology development. Propulsion system performance is tied to the achievability of these missions and the requirements of other developing technologies that will be required. Nuclear thermal propulsion offers a significant improvement over chemical propulsion while still achieving high levels of thrust. Opportunities exist; however, to build upon what would be considered a standard nuclear thermal engine to attain improved performance, thus further enabling deep space missions. This paper discuss the modeling of a nuclear thermal system integrated with an arc jet to further augment performance. The performance predictions and systems impacts are discussed.

Craters on Mars: Global Geometric Properties from Gridded MOLA Topography

NASA Technical Reports Server (NTRS)

Garvin, J. B.; Sakimoto, S. E. H.; Frawley, J. J.

2003-01-01

Impact craters serve as natural probes of the target properties of planetary crusts and the tremendous diversity of morphological expressions of such features on Mars attests to their importance for deciphering the history of crustal assembly, modification, and erosion. This paper summarizes the key findings associated with a five year long survey of the three-dimensional properties of approx. 6000 martian impact craters using finely gridded MOLA topography. Previous efforts have treated representative subpopulations, but this effort treats global properties from the largest survey of impact features from the perspective of their topography ever assimilated. With the Viking missions of the mid-1970 s, the most intensive and comprehensive robotic expeditions to any Deep Space location in the history of humanity were achieved, with scientifically stunning results associated with the morphology of impact craters. The relationships illustrated and suggest that martian impact features are remarkably sensitive to target properties and to the local depositional processes.

Broadband stimulated Raman spectroscopy in the deep ultraviolet region

NASA Astrophysics Data System (ADS)

Kuramochi, Hikaru; Fujisawa, Tomotsumi; Takeuchi, Satoshi; Tahara, Tahei

2017-09-01

We report broadband stimulated Raman measurements in the deep ultraviolet (DUV) region, which enables selective probing of the aromatic amino acid residues inside proteins through the resonance enhancement. We combine the narrowband DUV Raman pump pulse (<10 cm-1) at wavelengths as short as 240 nm and the broadband DUV probe pulse (>1000 cm-1) to realize stimulated Raman measurements covering a >1500 cm-1 spectral window. The stimulated Raman measurements for neat solvents, tryptophan, tyrosine, and glucose oxidase are performed using 240- and 290-nm Raman pump, highlighting the high potential of the DUV stimulated Raman probe for femtosecond time-resolved study of proteins.

The Future of NASA's Deep Space Network and Applications to Planetary Probe Missions

NASA Technical Reports Server (NTRS)

Deutsch, Leslie J.; Preston, Robert A.; Vrotsos, Peter

2010-01-01

NASA's Deep Space Network (DSN) has been an invaluable tool in the world's exploration of space. It has served the space-faring community for more than 45 years. The DSN has provided a primary communication pathway for planetary probes, either through direct- to-Earth links or through intermediate radio relays. In addition, its radiometric systems are critical to probe navigation and delivery to target. Finally, the radio link can also be used for direct scientific measurement of the target body ('radio science'). This paper will examine the special challenges in supporting planetary probe missions, the future evolution of the DSN and related spacecraft technology, the advantages and disadvantages of radio relay spacecraft, and the use of the DSN radio links for navigation and scientific measurements.

Identification of the spatial location of deep trap states in AlGaN/GaN heterostructures by surface photovoltage spectroscopy

NASA Astrophysics Data System (ADS)

Jana, Dipankar; Porwal, S.; Sharma, T. K.

2017-12-01

Spatial and spectral origin of deep level defects in molecular beam epitaxy grown AlGaN/GaN heterostructures are investigated by using surface photovoltage spectroscopy (SPS) and pump-probe SPS techniques. A deep trap center ∼1 eV above the valence band is observed in SPS measurements which is correlated with the yellow luminescence feature in GaN. Capture of electrons and holes is resolved by performing temperature dependent SPS and pump-probe SPS measurements. It is found that the deep trap states are distributed throughout the sample while their dominance in SPS spectra depends on the density, occupation probability of deep trap states and the background electron density of GaN channel layer. Dynamics of deep trap states associated with GaN channel layer is investigated by performing frequency dependent photoluminescence (PL) and SPS measurements. A time constant of few millisecond is estimated for the deep defects which might limit the dynamic performance of AlGaN/GaN based devices.

A passive low frequency instrument for radio wave sounding the subsurface oceans of the Jovian icy moons: An instrument concept

NASA Astrophysics Data System (ADS)

Hartogh, P.; Ilyushin, Ya. A.

2016-10-01

Exploration of subsurface oceans on Jovian icy moons is a key issue of the icy moons' geology. Electromagnetic wave propagation is the only way to probe their icy mantles from the orbit. In the present paper, a principal concept of a passive interferometric instrument for deep sounding of the icy moons' crust is proposed. Its working principle is measuring and correlating Jupiter's radio wave emissions with reflections from the deep sub-surface of the icy moons. A number of the functional aspects of the proposed experiment are studied, in particular, impact of the wave scattering on the surface terrain on the instrument performance and digital sampling of the noisy signal. Results of the test of the laboratory prototype of the instrument are also presented in the paper.

Probing the transition from shallow to deep convection

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kuang, Zhiming; Gentine, Pierre

2016-05-01

In this funded project we highlighted the components necessary for the transition from shallow to deep convection. In particular we defined a prototype of shallow to deep convection, which is currently being implemented in the NASA GISS model. We also tried to highlight differences between land and oceanic convection.

The Galileo Probe: How it Has Changed Our Understanding of Jupiter

NASA Technical Reports Server (NTRS)

Young, Richard E.

2003-01-01

The Galileo Mission to Jupiter, which arrived in December of 1995, provided the first study by an orbiter, and the first in-situ sampling via an entry probe, of an outer planet atmosphere. The rationale for an entry probe is that, even from an orbiter, remote sensing of the jovian atmosphere could not adequately retrieve the information desired. This paper provides a current summary of the most significant aspects of the data returned from the Galileo entry probe. As a result of the probe measurements, there has been a reassessment of our understanding of outer planet formation and evolution of the solar system. The primary scientific objective of the Galileo probe was to determine the composition of the jovian atmosphere, which from remote sensing remained either very uncertain, or completely unknown, with respect to several key elements. The probe found that the global He mass fraction is. significantly above the value reported from the Voyager Jupiter flybys but is slightly below the protosolar value, implying that there has been some settling of He to the deep jovian interior. The probe He measurements have also led to a reevaluation of the Voyager He mass fraction for Saturn, which is now determined to be much closer to that of Jupiter. The elements C, N, S, Ar, Kr, Xe were all found to have global abundances approximately 3 times their respective solar abundances. This result has raised a number of fundamental issues with regard to properties of planetesimals and the solar nebula at the time of giant planet formation. Ne, on the other hand, was found to be highly depleted, probably as the result of it being carried along with helium as helium settles towards the deep interior. The global abundance of O was not obtained by the probe because of the influence of local processes at the probe entry site (PES), processes which depleted condensible species, in this case H2O, well below condensation levels. Other condensible species, namely NH3 and H2S, were similarly affected but attained their deep equilibrium mixing ratios before the maximum depth sampled by the probe. Processes that might be capable of producing such effects on the condensibles are still under investigation. Measured isotopic ratios of noble gases and other heavy elements are solar, and (D + (Sup 3)He)/H is the same to within measurement uncertainties as in the local interstellar medium. No thick clouds were detected, and in particular no significant water cloud, but the PES location clearly affected the probe measurements of clouds. In fact, the probe data must be understood in the context of the location of the PES, which was within what is termed a 5 micron hot spot, a local clearing in the clouds that is bright near the 5 microns spectral region. The thermal structure at the PES was determined from approximately 1000 km above the 1 bar pressure level (10(exp -9 bars)) to 132 km 1 bar (22bars). The probe showed the atmosphere to have a generally sub-adiabatic temperature gradient (static stability) of = 0.1 K/km to as deep as the probe made measurements. In the upper atmosphere the probe derived a maximum positive vertical temperature gradient of approximately 5 K/km, and maximum temperature of = 900 K. The energy sources producing the warm upper atmosphere have yet to be completely identified. At first glance, Doppler tracking of the probe indicates that the long observed cloud level zonal winds extend to levels at least as deep as the probe made measurements. Zonal wind increases from = 80 m/s at pressures less than a bar to about 180 m/s near 5 bars, and remains approximately constant with depth thereafter. However, there is a question as to whether the winds measured from probe tracking are representative of the general wind field, or are considerably influenced by localized winds associated with the PES.

Minority Carrier Electron Traps in CZTSSe Solar Cells Characterized by DLTS and DLOS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kheraj, V.; Lund, E. A.; Caruso, A. E.

2016-11-21

We report observations of minority carrier interactions with deep levels in 6-8% efficient Cu2ZnSn(S, Se)4 (CZTSSe) devices using conventional and minority deep level transient spectroscopy (DLTS) and deep level optical spectroscopy (DLOS). Directly observing defect interactions with minority carriers is critical to understanding the recombination impact of deep levels. In devices with Cu2ZnSn(S, Se)4 nanoparticle ink absorber layers we identify a mid-gap state capturing and emitting minority electrons. It is 590+/-50 meV from the conduction band mobility edge, has a concentration near 1015/cm3, and has an apparent electron capture cross section ~10-14 cm2. We conclude that, while energetically positioned nearly-ideallymore » to be a recombination center, these defects instead act as electron traps because of a smaller hole cross-section. In CZTSe devices produced using coevaporation, we used minority carrier DLTS on traditional samples as well as ones with transparent Ohmic back contacts. These experiments demonstrate methods for unambiguously probing minority carrier/defect interactions in solar cells in order to establish direct links between defect energy level observations and minority carrier lifetimes. Furthermore, we demonstrate the use of steady-state device simulation to aid in the interpretation of DLTS results e.g. to put bounds on the complimentary carrier cross section even in the absence its direct measurement. This combined experimental and theoretical approach establishes rigorous bounds on the impact on carrier lifetime and Voc of defects observed with DLTS as opposed to, for example, assuming that all deep states act as strong recombination centers.« less

The Deep Space Network. An instrument for radio navigation of deep space probes

NASA Technical Reports Server (NTRS)

Renzetti, N. A.; Jordan, J. F.; Berman, A. L.; Wackley, J. A.; Yunck, T. P.

1982-01-01

The Deep Space Network (DSN) network configurations used to generate the navigation observables and the basic process of deep space spacecraft navigation, from data generation through flight path determination and correction are described. Special emphasis is placed on the DSN Systems which generate the navigation data: the DSN Tracking and VLBI Systems. In addition, auxiliary navigational support functions are described.

Deep inelastic scattering as a probe of entanglement

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kharzeev, Dmitri E.; Levin, Eugene M.

Using nonlinear evolution equations of QCD, we compute the von Neumann entropy of the system of partons resolved by deep inelastic scattering at a given Bjorken x and momentum transfer q 2 = - Q 2 . We interpret the result as the entropy of entanglement between the spatial region probed by deep inelastic scattering and the rest of the proton. At small x the relation between the entanglement entropy S ( x ) and the parton distribution x G ( x ) becomes very simple: S ( x ) = ln [ x G ( x ) ] .more » In this small x , large rapidity Y regime, all partonic microstates have equal probabilities—the proton is composed by an exponentially large number exp ( Δ Y ) of microstates that occur with equal and exponentially small probabilities exp ( - Δ Y ) , where Δ is defined by x G ( x ) ~ 1 / x Δ . For this equipartitioned state, the entanglement entropy is maximal—so at small x , deep inelastic scattering probes a maximally entangled state. Here, we propose the entanglement entropy as an observable that can be studied in deep inelastic scattering. This will then require event-by-event measurements of hadronic final states, and would allow to study the transformation of entanglement entropy into the Boltzmann one. We estimate that the proton is represented by the maximally entangled state at x ≤ 10 -3 ; this kinematic region will be amenable to studies at the Electron Ion Collider.« less

Deep inelastic scattering as a probe of entanglement

DOE PAGES

Kharzeev, Dmitri E.; Levin, Eugene M.

2017-06-03

Using nonlinear evolution equations of QCD, we compute the von Neumann entropy of the system of partons resolved by deep inelastic scattering at a given Bjorken x and momentum transfer q 2 = - Q 2 . We interpret the result as the entropy of entanglement between the spatial region probed by deep inelastic scattering and the rest of the proton. At small x the relation between the entanglement entropy S ( x ) and the parton distribution x G ( x ) becomes very simple: S ( x ) = ln [ x G ( x ) ] .more » In this small x , large rapidity Y regime, all partonic microstates have equal probabilities—the proton is composed by an exponentially large number exp ( Δ Y ) of microstates that occur with equal and exponentially small probabilities exp ( - Δ Y ) , where Δ is defined by x G ( x ) ~ 1 / x Δ . For this equipartitioned state, the entanglement entropy is maximal—so at small x , deep inelastic scattering probes a maximally entangled state. Here, we propose the entanglement entropy as an observable that can be studied in deep inelastic scattering. This will then require event-by-event measurements of hadronic final states, and would allow to study the transformation of entanglement entropy into the Boltzmann one. We estimate that the proton is represented by the maximally entangled state at x ≤ 10 -3 ; this kinematic region will be amenable to studies at the Electron Ion Collider.« less

Deep Space Network capabilities for receiving weak probe signals

NASA Technical Reports Server (NTRS)

Asmar, Sami; Johnston, Doug; Preston, Robert

2004-01-01

This paper will describe the capability and highlight the cases of the critical communications for the Mars rovers and Saturn Orbit Insertion and preparation radio tracking of the Huygens probe at (non-DSN) radio telescopes.

Molecular imaging needles: dual-modality optical coherence tomography and fluorescence imaging of labeled antibodies deep in tissue

PubMed Central

Scolaro, Loretta; Lorenser, Dirk; Madore, Wendy-Julie; Kirk, Rodney W.; Kramer, Anne S.; Yeoh, George C.; Godbout, Nicolas; Sampson, David D.; Boudoux, Caroline; McLaughlin, Robert A.

2015-01-01

Molecular imaging using optical techniques provides insight into disease at the cellular level. In this paper, we report on a novel dual-modality probe capable of performing molecular imaging by combining simultaneous three-dimensional optical coherence tomography (OCT) and two-dimensional fluorescence imaging in a hypodermic needle. The probe, referred to as a molecular imaging (MI) needle, may be inserted tens of millimeters into tissue. The MI needle utilizes double-clad fiber to carry both imaging modalities, and is interfaced to a 1310-nm OCT system and a fluorescence imaging subsystem using an asymmetrical double-clad fiber coupler customized to achieve high fluorescence collection efficiency. We present, to the best of our knowledge, the first dual-modality OCT and fluorescence needle probe with sufficient sensitivity to image fluorescently labeled antibodies. Such probes enable high-resolution molecular imaging deep within tissue. PMID:26137379

M13 phage-functionalized single-walled carbon nanotubes as nanoprobes for second near-infrared window fluorescence imaging of targeted tumors.

PubMed

Yi, Hyunjung; Ghosh, Debadyuti; Ham, Moon-Ho; Qi, Jifa; Barone, Paul W; Strano, Michael S; Belcher, Angela M

2012-03-14

Second near-infrared (NIR) window light (950-1400 nm) is attractive for in vivo fluorescence imaging due to its deep penetration depth in tissues and low tissue autofluorescence. Here we show genetically engineered multifunctional M13 phage can assemble fluorescent single-walled carbon nanotubes (SWNTs) and ligands for targeted fluorescence imaging of tumors. M13-SWNT probe is detectable in deep tissues even at a low dosage of 2 μg/mL and up to 2.5 cm in tissue-like phantoms. Moreover, targeted probes show specific and up to 4-fold improved uptake in prostate specific membrane antigen positive prostate tumors compared to control nontargeted probes. This M13 phage-based second NIR window fluorescence imaging probe has great potential for specific detection and therapy monitoring of hard-to-detect areas. © 2012 American Chemical Society

M13 phage-functionalized single-walled carbon nanotubes as nanoprobes for second near-infrared window fluorescence imaging of targeted tumors

PubMed Central

HAM, MOON-HO; QI, JIFA; BARONE, PAUL W.; STRANO, MICHAEL S.; BELCHER, ANGELA M.

2014-01-01

Second near-infrared (NIR) window light (950-1,400 nm) is attractive for in vivo fluorescence imaging due to its deep penetration depth in tissues and low tissue autofluorescence. Here we show genetically engineered multifunctional M13 phage can assemble fluorescent single-walled carbon nanotubes (SWNTs) and ligands for targeted fluorescence imaging of tumors. M13-SWNT probe is detectable in deep tissues even at a low dosage of 2 μg/mL and up to 2.5 cm in tissue-like phantoms. Moreover, targeted probes show specific and up to four-fold improved uptake in prostate specific membrane antigen positive prostate tumors compared to control non-targeted probes. This M13 phage-based second NIR window fluorescence imaging probe has great potential for specific detection and therapy monitoring of hard-to-detect areas. PMID:22268625

Parity Violation in Deep Inelastic Scattering in Hall C at JLab

NASA Astrophysics Data System (ADS)

Dalton, Mark Macrae; Keppel, Cynthia; Paschke, Kent

2017-09-01

The measurement of parity-violation in inclusive electron deep inelastic scattering (DIS) from a proton or deuteron target can be used to study the flavor structure of the nucleon. While valence quark parton distribution functions (PDF) can be probed in high- x measurements such as with the proposed SoLID spectrometer, complementary measurements are possible at moderate x 0.1 where the sea quarks may still play a significant role. In particular, such measurements would provide a cleanly interpretable measurement of the strange quark PDF. These measurements are possible with the upgraded CEBAF accelerator at JLab and do not require significant new experimental hardware. The prospects and potential impacts of such a measurement will be presented. This material is based upon work supported by the U.S. Department of Energy, Office of Science, Office of Nuclear Physics under Contract DE-AC05-06OR23177 and DE-FG02-07ER41522.

Clutter elimination for deep clinical optoacoustic imaging using localised vibration tagging (LOVIT)☆

PubMed Central

Jaeger, Michael; Bamber, Jeffrey C.; Frenz, Martin

2013-01-01

This paper investigates a novel method which allows clutter elimination in deep optoacoustic imaging. Clutter significantly limits imaging depth in clinical optoacoustic imaging, when irradiation optics and ultrasound detector are integrated in a handheld probe for flexible imaging of the human body. Strong optoacoustic transients generated at the irradiation site obscure weak signals from deep inside the tissue, either directly by propagating towards the probe, or via acoustic scattering. In this study we demonstrate that signals of interest can be distinguished from clutter by tagging them at the place of origin with localised tissue vibration induced by the acoustic radiation force in a focused ultrasonic beam. We show phantom results where this technique allowed almost full clutter elimination and thus strongly improved contrast for deep imaging. Localised vibration tagging by means of acoustic radiation force is especially promising for integration into ultrasound systems that already have implemented radiation force elastography. PMID:25302147

A silicon-based microelectrode array with a microdrive for monitoring brainstem regions of freely moving rats

NASA Astrophysics Data System (ADS)

Márton, G.; Baracskay, P.; Cseri, B.; Plósz, B.; Juhász, G.; Fekete, Z.; Pongrácz, A.

2016-04-01

Objective. Exploring neural activity behind synchronization and time locking in brain circuits is one of the most important tasks in neuroscience. Our goal was to design and characterize a microelectrode array (MEA) system specifically for obtaining in vivo extracellular recordings from three deep-brain areas of freely moving rats, simultaneously. The target areas, the deep mesencephalic reticular-, pedunculopontine tegmental- and pontine reticular nuclei are related to the regulation of sleep-wake cycles. Approach. The three targeted nuclei are collinear, therefore a single-shank MEA was designed in order to contact them. The silicon-based device was equipped with 3*4 recording sites, located according to the geometry of the brain regions. Furthermore, a microdrive was developed to allow fine actuation and post-implantation relocation of the probe. The probe was attached to a rigid printed circuit board, which was fastened to the microdrive. A flexible cable was designed in order to provide not only electronic connection between the probe and the amplifier system, but sufficient freedom for the movements of the probe as well. Main results. The microdrive was stable enough to allow precise electrode targeting into the tissue via a single track. The microelectrodes on the probe were suitable for recording neural activity from the three targeted brainstem areas. Significance. The system offers a robust solution to provide long-term interface between an array of precisely defined microelectrodes and deep-brain areas of a behaving rodent. The microdrive allowed us to fine-tune the probe location and easily scan through the regions of interest.

SinoProbe - A Multidisciplinary Research Program of Earth Sciences in China (Invited)

NASA Astrophysics Data System (ADS)

Dong, S.; Li, T.

2010-12-01

China occupies a large region of central and eastern Asia and holds keys to resolving several first-order problems in Earth Sciences. Besides the importance in Earth Science research, the rapid growth of Chinese economy also demands a comprehensive and systematic evaluation of its natural resources and the impacts of geohazards on its societal development. In order to address the above issues, the Chinese government had initiated a new multidisciplinary research project in Earth Sciences - the SinoProbe Program. Its fundamental goal is to determine the three-dimensional structure, composition distribution, and geological evolution of the Chinese continental lithosphere. The results of the SinoProbe Program are expected to have broad impacts on the Chinese society and economy. In particular, the program will greatly enhance our current understanding on (1) the forming and distribution of mineral resources in the nation, (2) the locations and recurrence histories of major active fault zones capable of generating large earthquakes in highly populated regions, and (3) the distribution of major hazard-prone regions induced by geological processes. In 2009, more than 720 investigators and 70 engineers from Chinese institutions are currently involved with the research program. Sinoprobe hope that the joint forces by Chinese and international researchers will bring in modern approaches, new analytical tools, and advanced exploration technology into the successful operation of the program. In past year, 1,960km long seismic reflection profiling with broadband seismological studies and MT surveys separated from 6 profiles in China continent have completed. MT array coved the North China craton by 1°×1° network and 3-D exploration in larger ore deposits in selected area were carried out. A scientific drilling area operated in Tibet. We started to establish a geochemical reference framework for the values of 76 elements in a grid network with data-point spacing of 160 km in China. Some stress monitoring were centered in the Beijing and the southeastern margin of the Qinghai-Tibet Plateau regions. Also, SinoProbe begin to establish a high-performance calculation platform that will consider coupling processes between deformation and thermal evolution in the lithosphere. Meanwhile, data integration and data dissemination is going to stored. Finally, SinoProbe will also devote to develop new technologies, innovative methods, data integration platforms, and modern equipments for deep Earth and mineral-deposit explorations. In summary, SinoProbe is a multi-year and multidisciplinary research program to be carried in China with 9 projects and 49 sub-projects. It will integrate geological, geophysical, geochemical, and modern exploration technology to examine the deep Earth structures and their evolution in China. The results will undoubtedly contribute to the improvement of our current understanding of the Eurasia continent in particular and the Earth in general.

The JPL optical communications telescope laboratory (OCTL) test bed for the future optical Deep Space Network

NASA Technical Reports Server (NTRS)

Wilson, K. E.; Page, N.; Wu, J.; Srinivasan, M.

2003-01-01

Relative to RF, the lower power-consumption and lower mass of high bandwidth optical telecommunications make this technology extremely attractive for returning data from future NASA/JPL deep space probes.

The Galileo probe Doppler wind experiment: Measurement of the deep zonal winds on Jupiter

NASA Astrophysics Data System (ADS)

Atkinson, David H.; Pollack, James B.; Seiff, Alvin

1998-09-01

During its descent into the upper atmosphere of Jupiter, the Galileo probe transmitted data to the orbiter for 57.5 min. Accurate measurements of the probe radio frequency, driven by an ultrastable oscillator, allowed an accurate time history of the probe motions to be reconstructed. Removal from the probe radio frequency profile of known Doppler contributions, including the orbiter trajectory, the probe descent velocity, and the rotation of Jupiter, left a measurable frequency residual due to Jupiter's zonal winds, and microdynamical motion of the probe from spin, swing under the parachute, atmospheric turbulence, and aerodynamic buffeting. From the assumption of the dominance of the zonal horizontal winds, the frequency residuals were inverted and resulted in the first in situ measurements of the vertical profile of Jupiter's deep zonal winds. A number of error sources with the capability of corrupting the frequency measurements or the interpretation of the frequency residuals were considered using reasonable assumptions and calibrations from prelaunch and in-flight testing. It is found that beneath the cloud tops (about 700 mbar) the winds are prograde and rise rapidly to 170 m/s at 4 bars. Beyond 4 bars to the depth at which the link with the probe was lost, nearly 21 bars, the winds remain constant and strong. Corrections for the high temperatures encountered by the probe have recently been completed and provide no evidence of diminishing or strengthening of the zonal wind profile in the deeper regions explored by the Galileo probe.

Planetary cubesats - mission architectures

NASA Astrophysics Data System (ADS)

Bousquet, Pierre W.; Ulamec, Stephan; Jaumann, Ralf; Vane, Gregg; Baker, John; Clark, Pamela; Komarek, Tomas; Lebreton, Jean-Pierre; Yano, Hajime

2016-07-01

Miniaturisation of technologies over the last decade has made cubesats a valid solution for deep space missions. For example, a spectacular set 13 cubesats will be delivered in 2018 to a high lunar orbit within the frame of SLS' first flight, referred to as Exploration Mission-1 (EM-1). Each of them will perform autonomously valuable scientific or technological investigations. Other situations are encountered, such as the auxiliary landers / rovers and autonomous camera that will be carried in 2018 to asteroid 1993 JU3 by JAXA's Hayabusas 2 probe, and will provide complementary scientific return to their mothership. In this case, cubesats depend on a larger spacecraft for deployment and other resources, such as telecommunication relay or propulsion. For both situations, we will describe in this paper how cubesats can be used as remote observatories (such as NEO detection missions), as technology demonstrators, and how they can perform or contribute to all steps in the Deep Space exploration sequence: Measurements during Deep Space cruise, Body Fly-bies, Body Orbiters, Atmospheric probes (Jupiter probe, Venus atmospheric probes, ..), Static Landers, Mobile landers (such as balloons, wheeled rovers, small body rovers, drones, penetrators, floating devices, …), Sample Return. We will elaborate on mission architectures for the most promising concepts where cubesat size devices offer an advantage in terms of affordability, feasibility, and increase of scientific return.

Cost and Performance Comparison of an Earth-Orbiting Optical Communication Relay Transceiver and a Ground-Based Optical Receiver Subnet

NASA Technical Reports Server (NTRS)

Wilson, K. E.; Wright, M.; Cesarone, R.; Ceniceros, J.; Shea, K.

2003-01-01

Optical communications can provide high-data-rate telemetry from deep-space probes with subsystems that have lower mass, consume less power, and are smaller than their radio frequency (RF) counterparts. However, because optical communication is more a.ected by weather than is RF communication, it requires groundstation site diversity to mitigate the adverse e.ects of inclement weather on the link. An optical relay satellite is not a.ected by weather and can provide 24-hour coverage of deep-space probes. Using such a relay satellite for the deep-space link and an 8.4-GHz (X-band) link to a ground station would support high-data-rate links from small deep-space probes with very little link loss due to inclement weather. We have reviewed past JPL-funded work on RF and optical relay satellites, and on proposed clustered and linearly dispersed optical subnets. Cost comparisons show that the life cycle costs of a 7-m optical relay station based on the heritage of the Next Generation Space Telescope is comparable to that of an 8-station subnet of 10- m optical ground stations. This makes the relay link an attractive option vis- a-vis a ground-station network.

Cost and Performance Comparison of an Earth-Orbiting Optical Communication Relay Transceiver and a Ground-Based Optical Receiver Subnet

NASA Astrophysics Data System (ADS)

Wilson, K. E.; Wright, M.; Cesarone, R.; Ceniceros, J.; Shea, K.

2003-01-01

Optical communications can provide high-data-rate telemetry from deep-space probes with subsystems that have lower mass, consume less power, and are smaller than their radio frequency (RF) counterparts. However, because optical communication is more affected by weather than is RF communication, it requires ground station site diversity to mitigate the adverse effects of inclement weather on the link. An optical relay satellite is not affected by weather and can provide 24-hour coverage of deep-space probes. Using such a relay satellite for the deep-space link and an 8.4-GHz (X-band) link to a ground station would support high-data-rate links from small deep-space probes with very little link loss due to inclement weather. We have reviewed past JPL-funded work on RF and optical relay satellites, and on proposed clustered and linearly dispersed optical subnets. Cost comparisons show that the life cycle costs of a 7-m optical relay station based on the heritage of the Next Generation Space Telescope is comparable to that of an 8-station subnet of 10-m optical ground stations. This makes the relay link an attractive option vis-a-vis a ground station network.

Deep brain two-photon NIR fluorescence imaging for study of Alzheimer's disease

NASA Astrophysics Data System (ADS)

Chen, Congping; Liang, Zhuoyi; Zhou, Biao; Ip, Nancy Y.; Qu, Jianan Y.

2018-02-01

Amyloid depositions in the brain represent the characteristic hallmarks of Alzheimer's disease (AD) pathology. The abnormal accumulation of extracellular amyloid-beta (Aβ) and resulting toxic amyloid plaques are considered to be responsible for the clinical deficits including cognitive decline and memory loss. In vivo two-photon fluorescence imaging of amyloid plaques in live AD mouse model through a chronic imaging window (thinned skull or craniotomy) provides a mean to greatly facilitate the study of the pathological mechanism of AD owing to its high spatial resolution and long-term continuous monitoring. However, the imaging depth for amyloid plaques is largely limited to upper cortical layers due to the short-wavelength fluorescence emission of commonly used amyloid probes. In this work, we reported that CRANAD-3, a near-infrared (NIR) probe for amyloid species with excitation wavelength at 900 nm and emission wavelength around 650 nm, has great advantages over conventionally used probes and is well suited for twophoton deep imaging of amyloid plaques in AD mouse brain. Compared with a commonly used MeO-X04 probe, the imaging depth of CRANAD-3 is largely extended for open skull cranial window. Furthermore, by using two-photon excited fluorescence spectroscopic imaging, we characterized the intrinsic fluorescence of the "aging pigment" lipofuscin in vivo, which has distinct spectra from CRANAD-3 labeled plaques. This study reveals the unique potential of NIR probes for in vivo, high-resolution and deep imaging of brain amyloid in Alzheimer's disease.

Deep Space Network Capabilities for Receiving Weak Probe Signals

NASA Technical Reports Server (NTRS)

Asmar, Sami; Johnston, Doug; Preston, Robert

2005-01-01

Planetary probes can encounter mission scenarios where communication is not favorable during critical maneuvers or emergencies. Launch, initial acquisition, landing, trajectory corrections, safing. Communication challenges due to sub-optimum antenna pointing or transmitted power, amplitude/frequency dynamics, etc. Prevent lock-up on signal and extraction of telemetry. Examples: loss of Mars Observer, nutation of Ulysses, Galileo antenna, Mars Pathfinder and Mars Exploration Rovers Entry, Descent, and Landing, and the Cassini Saturn Orbit Insertion. A Deep Space Network capability to handle such cases has been used successfully to receive signals to characterize the scenario. This paper will describe the capability and highlight the cases of the critical communications for the Mars rovers and Saturn Orbit Insertion and preparation radio tracking of the Huygens probe at (non-DSN) radio telescopes.

Multiplexed 3D FRET imaging in deep tissue of live embryos

PubMed Central

Zhao, Ming; Wan, Xiaoyang; Li, Yu; Zhou, Weibin; Peng, Leilei

2015-01-01

Current deep tissue microscopy techniques are mostly restricted to intensity mapping of fluorophores, which significantly limit their applications in investigating biochemical processes in vivo. We present a deep tissue multiplexed functional imaging method that probes multiple Förster resonant energy transfer (FRET) sensors in live embryos with high spatial resolution. The method simultaneously images fluorescence lifetimes in 3D with multiple excitation lasers. Through quantitative analysis of triple-channel intensity and lifetime images, we demonstrated that Ca2+ and cAMP levels of live embryos expressing dual FRET sensors can be monitored simultaneously at microscopic resolution. The method is compatible with a broad range of FRET sensors currently available for probing various cellular biochemical functions. It opens the door to imaging complex cellular circuitries in whole live organisms. PMID:26387920

A Point Spread Function for the EPOXI Mission

NASA Technical Reports Server (NTRS)

Barry, Richard K.

2010-01-01

The Extrasolar Planet Observation Characterization and the Deep Impact Extended Investigation missions (EPOXI) are currently observing the transits of exoplanets, two comet nuclei at short range, and the Earth and Mars using the High Resolution Instrument (HRI) - a 0.3 m f/35 telescope on the Deep Impact probe. The HRI is in a permanently defocused state with the instrument pOint of focus about 0.6 cm before the focal plane due to the use of a reference flat mirror that took a power during ground thermal-vacuum testing. Consequently, the point spread function (PSF) covers approximately nine pixels FWHM and is characterized by a patch with three-fold symmetry due to the three-point support structures of the primary and secondary mirrors. The PSF is also strongly color dependent varying in shape and size with change in filtration and target color. While defocus is highly desirable for exoplanet transit observations to limit sensitivity to intra-pixel variation, it is suboptimal for observations of spatially resolved targets. Consequently, all images used in our analysis of such objects were deconvolved with an instrument PSF. The instrument PSF is also being used to optimize transit analysis. We discuss development and usage of an instrument PSF for these observations.

Focused Crawling of the Deep Web Using Service Class Descriptions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rocco, D; Liu, L; Critchlow, T

2004-06-21

Dynamic Web data sources--sometimes known collectively as the Deep Web--increase the utility of the Web by providing intuitive access to data repositories anywhere that Web access is available. Deep Web services provide access to real-time information, like entertainment event listings, or present a Web interface to large databases or other data repositories. Recent studies suggest that the size and growth rate of the dynamic Web greatly exceed that of the static Web, yet dynamic content is often ignored by existing search engine indexers owing to the technical challenges that arise when attempting to search the Deep Web. To address thesemore » challenges, we present DynaBot, a service-centric crawler for discovering and clustering Deep Web sources offering dynamic content. DynaBot has three unique characteristics. First, DynaBot utilizes a service class model of the Web implemented through the construction of service class descriptions (SCDs). Second, DynaBot employs a modular, self-tuning system architecture for focused crawling of the DeepWeb using service class descriptions. Third, DynaBot incorporates methods and algorithms for efficient probing of the Deep Web and for discovering and clustering Deep Web sources and services through SCD-based service matching analysis. Our experimental results demonstrate the effectiveness of the service class discovery, probing, and matching algorithms and suggest techniques for efficiently managing service discovery in the face of the immense scale of the Deep Web.« less

Probes for measuring noise current in an electronic cable

NASA Technical Reports Server (NTRS)

Lundy, C. C.

1974-01-01

Electromagnetic interference in deep-space network receiver is often caused by stray coupling from power lines. These stray signals create potential differences between ground terminals, which leads to excessive noise in receiver circuits. Pair of probes detect and measure noise currents in conductors.

Using Jupiter's gravitational field to probe the Jovian convective dynamo.

PubMed

Kong, Dali; Zhang, Keke; Schubert, Gerald

2016-03-23

Convective motion in the deep metallic hydrogen region of Jupiter is believed to generate its magnetic field, the strongest in the solar system. The amplitude, structure and depth of the convective motion are unknown. A promising way of probing the Jovian convective dynamo is to measure its effect on the external gravitational field, a task to be soon undertaken by the Juno spacecraft. We calculate the gravitational signature of non-axisymmetric convective motion in the Jovian metallic hydrogen region and show that with sufficiently accurate measurements it can reveal the nature of the deep convection.

Using Jupiter’s gravitational field to probe the Jovian convective dynamo

PubMed Central

Kong, Dali; Zhang, Keke; Schubert, Gerald

2016-01-01

Convective motion in the deep metallic hydrogen region of Jupiter is believed to generate its magnetic field, the strongest in the solar system. The amplitude, structure and depth of the convective motion are unknown. A promising way of probing the Jovian convective dynamo is to measure its effect on the external gravitational field, a task to be soon undertaken by the Juno spacecraft. We calculate the gravitational signature of non-axisymmetric convective motion in the Jovian metallic hydrogen region and show that with sufficiently accurate measurements it can reveal the nature of the deep convection. PMID:27005472

THE DEEP2 GALAXY REDSHIFT SURVEY: THE VORONOI-DELAUNAY METHOD CATALOG OF GALAXY GROUPS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gerke, Brian F.; Newman, Jeffrey A.; Davis, Marc

2012-05-20

We present a public catalog of galaxy groups constructed from the spectroscopic sample of galaxies in the fourth data release from the Deep Extragalactic Evolutionary Probe 2 (DEEP2) Galaxy Redshift Survey, including the Extended Groth Strip (EGS). The catalog contains 1165 groups with two or more members in the EGS over the redshift range 0 < z < 1.5 and 1295 groups at z > 0.6 in the rest of DEEP2. Twenty-five percent of EGS galaxies and fourteen percent of high-z DEEP2 galaxies are assigned to galaxy groups. The groups were detected using the Voronoi-Delaunay method (VDM) after it hasmore » been optimized on mock DEEP2 catalogs following similar methods to those employed in Gerke et al. In the optimization effort, we have taken particular care to ensure that the mock catalogs resemble the data as closely as possible, and we have fine-tuned our methods separately on mocks constructed for the EGS and the rest of DEEP2. We have also probed the effect of the assumed cosmology on our inferred group-finding efficiency by performing our optimization on three different mock catalogs with different background cosmologies, finding large differences in the group-finding success we can achieve for these different mocks. Using the mock catalog whose background cosmology is most consistent with current data, we estimate that the DEEP2 group catalog is 72% complete and 61% pure (74% and 67% for the EGS) and that the group finder correctly classifies 70% of galaxies that truly belong to groups, with an additional 46% of interloper galaxies contaminating the catalog (66% and 43% for the EGS). We also confirm that the VDM catalog reconstructs the abundance of galaxy groups with velocity dispersions above {approx}300 km s{sup -1} to an accuracy better than the sample variance, and this successful reconstruction is not strongly dependent on cosmology. This makes the DEEP2 group catalog a promising probe of the growth of cosmic structure that can potentially be used for cosmological tests.« less

X-Ray Probes of Cosmic Star Formation History

NASA Technical Reports Server (NTRS)

Ghosh, Pranab; White, Nicholas E.

2001-01-01

We discuss the imprints left by a cosmological evolution of the star formation rate (SFR) on the evolution of X-ray luminosities Lx of normal galaxies, using the scheme earlier proposed by us, wherein the evolution of LX of a galaxy is driven by the evolution of its X-ray binary population. As indicated in our earlier work, the profile of Lx with redshift can both serve as a diagnostic probe of the SFR profile and constrain evolutionary models for X-ray binaries. We report here the first calculation of the expected evolution of X-ray luminosities of galaxies, updating our work by using a suite of more recently developed SFR profiles that span the currently plausible range. The first Chandra deep imaging results on Lx evolution are beginning to probe the SFR profile of bright spiral galaxies; the early results are consistent with predictions based on current SFR models. Using these new SFR profiles, the resolution of the "birthrate problem" of low-mass X-ray binaries and recycled, millisecond pulsars in terms of an evolving global SFR is more complete. We discuss the possible impact of the variations in the SFR profile of individual galaxies and galaxy types.

Probing Galaxy Formation and Evolution with Space Born Sub-Millimeter Telescopes

NASA Technical Reports Server (NTRS)

Dwek, Eli; Arendt, Richard G.; Moseley, Harvey; Benford, Dominic; Shafer, Richard; Mather, John; Oegerle, William (Technical Monitor)

2002-01-01

A major unresolved question in cosmology is how the complex system of galaxies we see in the present universe evolved from an almost perfectly smooth beginning. Multiwavelength observations of galaxies have revealed that a significant fraction of their UV-visible starlight is absorbed and reradiated by dust at infrared JR) and submillimeter wavelengths. The cumulative IR-submm. emission from galaxies since the epoch of recombination, the cosmic IR background, has recently been recorded by the COBE satellite. The COBE observations in combination with recent submm surveys conducted with the SCUBA on the 15 m JCMT have shown that most of the radiation from star formation that has taken place in the early stages of galaxy evolution is reradiated by dust at submm wavelengths. Therefore, submm telescopes offer a unique probe of the early stages of galaxy formation and evolution. This talk will: (1) consider the impact of telescope diameter on the depth of the survey (what redshift can be probed) at different wavelengths; (2) discuss the relative scientific merits of high-resolution narrow-field surveys versus lower resolution deep surveys; and (3) show how both strategies offer complementary information crucial to our understanding of the structure and evolution of galaxies in the universe.

Deep-red to near-infrared fluorescent dyes: Synthesis, photophysical properties, and application in cell imaging

NASA Astrophysics Data System (ADS)

Li, Qi; Liu, Weimin; Wu, Jiasheng; Zhou, Bingjiang; Niu, Guangle; Zhang, Hongyan; Ge, Jiechao; Wang, Pengfei

2016-07-01

More and more attention has been paid to the design of new fluorescent imaging agents with good photostability and water solubility, especially those with emissions in the deep-red and near-infrared regions. In this work, we designed and synthesized four novel fluorescent dyes with deep-red or NIR fluorescence by hybridizing coumarin and pyronin moieties based on our previous work. Introduction of carboxylic acid in the dyes not only imparted the dyes with water solubility but also provided a versatile sensing platform for designing the fluorescent probes and sensors of biomolecules. The photophysical properties of these new dyes were investigated through absorption and fluorescence spectroscopy. Cell imaging experiments showed that esterification products could selectively stain lysosomes with good photostability, thereby indicating that they could be useful in the development of fluorescent probes for bioimaging.

The Interaction between Very Deep Convection and Biomass Burning Plumes during DC3 and the Impact on the UTLS Region over The North Atlantic

NASA Astrophysics Data System (ADS)

Huntrieser, H.; Lichtenstern, M.; Scheibe, M.; Aufmhoff, H.; Schlager, H.; Heimerl, K.; Pucik, T.; Minikin, A.; Weinzierl, B.; Fütterer, D.; Pollack, I. B.; Peischl, J.; Ryerson, T. B.; Honomichl, S.; Hair, J. W.; Butler, C. F.; Schwartz, M. J.; Rappenglück, B.; Ackermann, L.; Pickering, K. E.; Cummings, K.; Biggerstaff, M. I.; Betten, D.; Barth, M. C.

2016-12-01

The Central United States is known to be a region where intense thunderstorms develop. During the Deep Convective Cloud and Chemistry Experiment (DC3) in summer 2012 a number of these imposing storms were investigated by airborne and ground-based measurements focusing on the chemistry, microphysics and dynamics in these unique storms. Here we report on aircraft penetrations of the anvil outflow of isolated supercells and organized mesoscale convective systems and the distribution of different trace species as e.g. CO, O3, and NOx. Conspicuously, the burning of several extended wildfires in New Mexico and Colorado, which emitted huge amounts of SO2 and black carbon (BC), significantly impacted the chemical composition within and nearby the probed thunderstorms. In several cases, overshooting thunderstorms developed that injected considerable amounts of pollutants into the lower stratosphere. Both in the lofted biomass burning plumes and in the thunderstorm outflow, O3 mixing ratios were frequently enhanced due to photochemical production and downward transport from the stratosphere; however, the latter process dominated the measured O3 enhancements in the storms. Here we present results from the local flights over Colorado, Oklahoma and Texas along with transit flights over the North Atlantic conducted by the German DLR Falcon research aircraft. In addition, microphysical measurements from radar, and remote trace species measurements (lidar and satellites) are used to demonstrate the strong air mass exchange in the UTLS region caused by the frequent occurrence of very deep convection over the Central U.S. The more general impact of these widespread, aged, and more or less invisible anvil outflows on the UTLS region downwind of the U.S. continent (North Atlantic) is discussed regarding chemistry, new particle formation, and radiation.

High Aspect-Ratio Neural Probes using Conventional Blade Dicing

NASA Astrophysics Data System (ADS)

Goncalves, S. B.; Ribeiro, J. F.; Silva, A. F.; Correia, J. H.

2016-10-01

Exploring deep neural circuits has triggered the development of long penetrating neural probes. Moreover, driven by brain displacement, the long neural probes require also a high aspect-ratio shafts design. In this paper, a simple and reproducible method of manufacturing long-shafts neural probes using blade dicing technology is presented. Results shows shafts up to 8 mm long and 200 µm wide, features competitive to the current state-of-art, being its outline simply accomplished by a single blade dicing program. Therefore, conventional blade dicing presents itself as a viable option to manufacture long neural probes.

Intrauterine photoacoustic and ultrasound imaging probe

NASA Astrophysics Data System (ADS)

Miranda, Christopher; Barkley, Joel; Smith, Barbara S.

2018-04-01

Intrauterine photoacoustic and ultrasound imaging are probe-based imaging modalities with translational potential for use in detecting endometrial diseases. This deep-tissue imaging probe design allows for the retrofitting of commercially available endometrial sampling curettes. The imaging probe presented here has a 2.92-mm diameter and approximate length of 26 cm, which allows for entry into the human endometrial cavity, making it possible to use photoacoustic imaging and high-resolution ultrasound to characterize the uterus. We demonstrate the imaging probes' ability to provide structural information of an excised pig uterus using ultrasound imaging and detect photoacoustic signals at a radial depth of 1 cm.

Providing Multi-Page Data Extraction Services with XWRAPComposer

DOE Office of Scientific and Technical Information (OSTI.GOV)

Liu, Ling; Zhang, Jianjun; Han, Wei

2008-04-30

Dynamic Web data sources – sometimes known collectively as the Deep Web – increase the utility of the Web by providing intuitive access to data repositories anywhere that Web access is available. Deep Web services provide access to real-time information, like entertainment event listings, or present a Web interface to large databases or other data repositories. Recent studies suggest that the size and growth rate of the dynamic Web greatly exceed that of the static Web, yet dynamic content is often ignored by existing search engine indexers owing to the technical challenges that arise when attempting to search the Deepmore » Web. To address these challenges, we present DYNABOT, a service-centric crawler for discovering and clustering Deep Web sources offering dynamic content. DYNABOT has three unique characteristics. First, DYNABOT utilizes a service class model of the Web implemented through the construction of service class descriptions (SCDs). Second, DYNABOT employs a modular, self-tuning system architecture for focused crawling of the Deep Web using service class descriptions. Third, DYNABOT incorporates methods and algorithms for efficient probing of the Deep Web and for discovering and clustering Deep Web sources and services through SCD-based service matching analysis. Our experimental results demonstrate the effectiveness of the service class discovery, probing, and matching algorithms and suggest techniques for efficiently managing service discovery in the face of the immense scale of the Deep Web.« less

Image-based deep learning for classification of noise transients in gravitational wave detectors

NASA Astrophysics Data System (ADS)

Razzano, Massimiliano; Cuoco, Elena

2018-05-01

The detection of gravitational waves has inaugurated the era of gravitational astronomy and opened new avenues for the multimessenger study of cosmic sources. Thanks to their sensitivity, the Advanced LIGO and Advanced Virgo interferometers will probe a much larger volume of space and expand the capability of discovering new gravitational wave emitters. The characterization of these detectors is a primary task in order to recognize the main sources of noise and optimize the sensitivity of interferometers. Glitches are transient noise events that can impact the data quality of the interferometers and their classification is an important task for detector characterization. Deep learning techniques are a promising tool for the recognition and classification of glitches. We present a classification pipeline that exploits convolutional neural networks to classify glitches starting from their time-frequency evolution represented as images. We evaluated the classification accuracy on simulated glitches, showing that the proposed algorithm can automatically classify glitches on very fast timescales and with high accuracy, thus providing a promising tool for online detector characterization.

Electronics for Deep Space Cryogenic Applications

NASA Technical Reports Server (NTRS)

Patterson, R. L.; Hammond, A.; Dickman, J. E.; Gerber, S. S.; Elbuluk, M. E.; Overton, E.

2002-01-01

Deep space probes and planetary exploration missions require electrical power management and control systems that are capable of efficient and reliable operation in very cold temperature environments. Typically, in deep space probes, heating elements are used to keep the spacecraft electronics near room temperature. The utilization of power electronics designed for and operated at low temperature will contribute to increasing efficiency and improving reliability of space power systems. At NASA Glenn Research Center, commercial-off-the-shelf devices as well as developed components are being investigated for potential use at low temperatures. These devices include semiconductor switching devices, magnetics, and capacitors. Integrated circuits such as digital-to-analog and analog-to-digital converters, DC/DC converters, operational amplifiers, and oscillators are also being evaluated. In this paper, results will be presented for selected analog-to-digital converters, oscillators, DC/DC converters, and pulse width modulation (PWM) controllers.

Note: long range and accurate measurement of deep trench microstructures by a specialized scanning tunneling microscope.

PubMed

Ju, Bing-Feng; Chen, Yuan-Liu; Zhang, Wei; Zhu, Wule; Jin, Chao; Fang, F Z

2012-05-01

A compact but practical scanning tunneling microscope (STM) with high aspect ratio and high depth capability has been specially developed. Long range scanning mechanism with tilt-adjustment stage is adopted for the purpose of adjusting the probe-sample relative angle to compensate the non-parallel effects. A periodical trench microstructure with a pitch of 10 μm has been successfully imaged with a long scanning range up to 2.0 mm. More innovatively, a deep trench with depth and step height of 23.0 μm has also been successfully measured, and slope angle of the sidewall can approximately achieve 67°. The probe can continuously climb the high step and exploring the trench bottom without tip crashing. The new STM could perform long range measurement for the deep trench and high step surfaces without image distortion. It enables accurate measurement and quality control of periodical trench microstructures.

Engineering of bacterial phytochromes for in vivo imaging (Conference Presentation)

NASA Astrophysics Data System (ADS)

Verkhusha, Vladislav; Shcherbakova, Daria M.; Kaberniuk, Andrii A.; Baloban, Mikhail

2017-03-01

Genetically encoded probes with absorbance and fluorescence spectra within a near-infrared tissue transparency window are preferable for deep-tissue imaging. On the basis of bacterial phytochromes we engineered several types of near-infrared absorbing probes for photoacoustic tomography and fluorescent probes for purely optical imaging. They can be used as protein and cell labels and as building blocks for biosensors. The probes enabled imaging of tumors and metastases, protein-protein interactions, RNA visualization, detection of apoptosis, cellular metabolites, signaling pathways and cell proliferation. The developed probes allow non-invasive visualization of biological processes across scales, from super-resolution microscopy to tissue and whole-body animal imaging.

The deep space network. [tracking and communication support for space probes

NASA Technical Reports Server (NTRS)

1974-01-01

The objectives, functions, and organization of the deep space network are summarized. Progress in flight project support, tracking and data acquisition research and technology, network engineering, hardware and software implementation, and operations is reported. Interface support for the Mariner Venus Mercury 1973 flight and Pioneer 10 and 11 missions is included.

Multi-MICE: Nuclear Powered Mobile Probes to Explore Deep Interiors of the Ice Sheets on Mars and the Jovian Moons

DOE Office of Scientific and Technical Information (OSTI.GOV)

Maise, George; Powell, James; Paniagua, John

2007-01-30

The multi-kilometer thick Polar Caps on Mars contain unique and important data about the multi-million year history of its climate, geology, meteorology, volcanology, cosmic ray and solar activity, and meteor impacts. They also may hold evidence of past life on Mars, including microbes, microfossils and biological chemicals. The objective of this paper is to describe a probe that can provide access to the data locked in the Polar Caps. The MICE (Mars Ice Cap Explorer) system would explore the Polar Cap interiors using mobile probes powered by compact, lightweight nuclear reactors. The probes would travel 100's of meters per daymore » along melt channels in the ice sheets created by hot water jets from the 500 kW(th) nuclear reactors, ascending and descending, either vertically or at an angle to the vertical, reaching bedrock at kilometers beneath the surface. The powerful reactor will be necessary to provide sufficient hot water at high velocity to penetrate the extensive horizontal dust/sand layers that separate layers of ice in the Mars Ice Caps. MICE reactors can operate at 500 kW(th) for more than 4 years, and much longer in practice, since power level will be much lower when the probes are investigating locations in detail at low or zero speed. Multiple probes, e.g. six, would be deployed in an interactive network, continuously communicating by RF and acoustic signals with each other and with the surface lander spacecraft. In turn, the lander would continuously communicate in real time, subject to speed of light delays, with scientists on Earth to transmit data and receive instructions for the MICE probes. Samples collected by the probes could be brought to the lander, for return to the Earth at the end of the mission.« less

Probing aerosol indirect effect on deep convection using idealized cloud-resolving simulations with parameterized large-scale dynamics.

NASA Astrophysics Data System (ADS)

Anber, U.; Wang, S.; Gentine, P.; Jensen, M. P.

2017-12-01

A framework is introduced to investigate the indirect impact of aerosol loading on tropical deep convection using 3-dimentional idealized cloud-system resolving simulations with coupled large-scale circulation. The large scale dynamics is parameterized using a spectral weak temperature gradient approximation that utilizes the dominant balance in the tropics between adiabatic cooling and diabatic heating. Aerosol loading effect is examined by varying the number concentration of nuclei (CCN) to form cloud droplets in the bulk microphysics scheme over a wide range from 30 to 5000 without including any radiative effect as the radiative cooling is prescribed at a constant rate, to isolate the microphysical effect. Increasing aerosol number concentration causes mean precipitation to decrease monotonically, despite the increase in cloud condensates. Such reduction in precipitation efficiency is attributed to reduction in the surface enthalpy fluxes, and not to the divergent circulation, as the gross moist stability remains unchanged. We drive a simple scaling argument based on the moist static energy budget, that enables a direct estimation of changes in precipitation given known changes in surfaces enthalpy fluxes and the constant gross moist stability. The impact on cloud hydrometers and microphysical properties is also examined and is consistent with the macro-physical picture.

Phase fluctuations model for EM wave propagation through solar scintillation at superior solar conjunction

NASA Astrophysics Data System (ADS)

Xu, Guanjun; Song, Zhaohui

2017-04-01

Traveling solar wind disturbances have a significant influence on radio wave characteristics during the superior solar conjunction communication. This paper considers the impact of solar scintillation on phase fluctuations of electromagnetic (EM) wave propagation during the superior solar conjunction. Based on the Geometric Optics approximation, the close-form approximation model for phase fluctuations is developed. Both effects of anisotropic temporal variations function of plasma irregularities and their power spectrum are presented and analyzed numerically. It is found that phase fluctuations rapidly decrease with increasing Sun-Earth-Probe angle and decrease with increasing frequency at the rate of 1/f2. Moreover, the role of various features of the solar wind irregularities and their influence on the EM wave characteristic parameters is studied and discussed. Finally, we study the phase fluctuations of typical cases in order to better understand the impact of phase fluctuations in future deep space communication scenarios during solar conjunction periods.

Probing the Solar System

ERIC Educational Resources Information Center

Wilkinson, John

2013-01-01

Humans have always had the vision to one day live on other planets. This vision existed even before the first person was put into orbit. Since the early space missions of putting humans into orbit around Earth, many advances have been made in space technology. We have now sent many space probes deep into the Solar system to explore the planets and…

Deep drilling; Probing beneath the earth's surface

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rosen, J.250

1991-06-01

This paper reports on boreholes from 4.5 to greater than 10 kilometers deep that are pushing back the boundaries of earth science as they yield information that is used to refine seismic surveys, chart the evolution of sedimentary basins and shield volcanos, and uncover important clues on the origin and migration of mantle-derived water and gas.

A nontoxic, photostable and high signal-to-noise ratio mitochondrial probe with mitochondrial membrane potential and viscosity detectivity

NASA Astrophysics Data System (ADS)

Chen, Yanan; Qi, Jianguo; Huang, Jing; Zhou, Xiaomin; Niu, Linqiang; Yan, Zhijie; Wang, Jianhong

2018-01-01

Herein, we reported a yellow emission probe 1-methyl-4-(6-morpholino-1, 3-dioxo-1H-benzo[de]isoquinolin-2(3H)-yl) pyridin-1-ium iodide which could specifically stain mitochondria in living immortalized and normal cells. In comparison to the common mitochondria tracker (Mitotracker Deep Red, MTDR), this probe was nontoxic, photostable and ultrahigh signal-to-noise ratio, which could real-time monitor mitochondria for a long time. Moreover, this probe also showed high sensitivity towards mitochondrial membrane potential and intramitochondrial viscosity change. Consequently, this probe was used for imaging mitochondria, detecting changes in mitochondrial membrane potential and intramitochondrial viscosity in physiological and pathological processes.

49 CFR 572.18 - Thorax.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 11. (b) When impacted by a test probe conforming to § 572.21(a) at 13 fps in accordance with... parallel to the face of the test probe. (4) Place the longitudinal centerline of the test probe to coincide with the designated impact point and align the test probe so that at impact its longitudinal centerline...

49 CFR 572.18 - Thorax.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 11. (b) When impacted by a test probe conforming to § 572.21(a) at 13 fps in accordance with... parallel to the face of the test probe. (4) Place the longitudinal centerline of the test probe to coincide with the designated impact point and align the test probe so that at impact its longitudinal centerline...

49 CFR 572.18 - Thorax.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 11. (b) When impacted by a test probe conforming to § 572.21(a) at 13 fps in accordance with... parallel to the face of the test probe. (4) Place the longitudinal centerline of the test probe to coincide with the designated impact point and align the test probe so that at impact its longitudinal centerline...

49 CFR 572.18 - Thorax.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 11. (b) When impacted by a test probe conforming to § 572.21(a) at 13 fps in accordance with... parallel to the face of the test probe. (4) Place the longitudinal centerline of the test probe to coincide with the designated impact point and align the test probe so that at impact its longitudinal centerline...

Deep brain stimulation of the subthalamic nucleus modulates sensitivity to decision outcome value in Parkinson’s disease

NASA Astrophysics Data System (ADS)

Seymour, Ben; Barbe, Michael; Dayan, Peter; Shiner, Tamara; Dolan, Ray; Fink, Gereon R.

2016-09-01

Deep brain stimulation (DBS) of the subthalamic nucleus in Parkinson’s disease is known to cause a subtle but important adverse impact on behaviour, with impulsivity its most widely reported manifestation. However, precisely which computational components of the decision process are modulated is not fully understood. Here we probe a number of distinct subprocesses, including temporal discount, outcome utility, instrumental learning rate, instrumental outcome sensitivity, reward-loss trade-offs, and perseveration. We tested 22 Parkinson’s Disease patients both on and off subthalamic nucleus deep brain stimulation (STN-DBS), while they performed an instrumental learning task involving financial rewards and losses, and an inter-temporal choice task for financial rewards. We found that instrumental learning performance was significantly worse following stimulation, due to modulation of instrumental outcome sensitivity. Specifically, patients became less sensitive to decision values for both rewards and losses, but without any change to the learning rate or reward-loss trade-offs. However, we found no evidence that DBS modulated different components of temporal impulsivity. In conclusion, our results implicate the subthalamic nucleus in a modulation of outcome value in experience-based learning and decision-making in Parkinson’s disease, suggesting a more pervasive role of the subthalamic nucleus in the control of human decision-making than previously thought.

Deep brain stimulation of the subthalamic nucleus modulates sensitivity to decision outcome value in Parkinson’s disease

PubMed Central

Seymour, Ben; Barbe, Michael; Dayan, Peter; Shiner, Tamara; Dolan, Ray; Fink, Gereon R.

2016-01-01

Deep brain stimulation (DBS) of the subthalamic nucleus in Parkinson’s disease is known to cause a subtle but important adverse impact on behaviour, with impulsivity its most widely reported manifestation. However, precisely which computational components of the decision process are modulated is not fully understood. Here we probe a number of distinct subprocesses, including temporal discount, outcome utility, instrumental learning rate, instrumental outcome sensitivity, reward-loss trade-offs, and perseveration. We tested 22 Parkinson’s Disease patients both on and off subthalamic nucleus deep brain stimulation (STN-DBS), while they performed an instrumental learning task involving financial rewards and losses, and an inter-temporal choice task for financial rewards. We found that instrumental learning performance was significantly worse following stimulation, due to modulation of instrumental outcome sensitivity. Specifically, patients became less sensitive to decision values for both rewards and losses, but without any change to the learning rate or reward-loss trade-offs. However, we found no evidence that DBS modulated different components of temporal impulsivity. In conclusion, our results implicate the subthalamic nucleus in a modulation of outcome value in experience-based learning and decision-making in Parkinson’s disease, suggesting a more pervasive role of the subthalamic nucleus in the control of human decision-making than previously thought. PMID:27624437

The Making of Deep Impact

NASA Image and Video Library

2006-10-19

This image shows NASA Deep Impact spacecraft being built at Ball Aerospace & Technologies Corporation, Boulder, Colo. on July 2, 2005. The spacecraft impactor was released from Deep Impact flyby spacecraft.

Ice-Borehole Probe

NASA Technical Reports Server (NTRS)

Behar, Alberto; Carsey, Frank; Lane, Arthur; Engelhardt, Herman

2006-01-01

An instrumentation system has been developed for studying interactions between a glacier or ice sheet and the underlying rock and/or soil. Prior borehole imaging systems have been used in well-drilling and mineral-exploration applications and for studying relatively thin valley glaciers, but have not been used for studying thick ice sheets like those of Antarctica. The system includes a cylindrical imaging probe that is lowered into a hole that has been bored through the ice to the ice/bedrock interface by use of an established hot-water-jet technique. The images acquired by the cameras yield information on the movement of the ice relative to the bedrock and on visible features of the lower structure of the ice sheet, including ice layers formed at different times, bubbles, and mineralogical inclusions. At the time of reporting the information for this article, the system was just deployed in two boreholes on the Amery ice shelf in East Antarctica and after successful 2000 2001 deployments in 4 boreholes at Ice Stream C, West Antarctica, and in 2002 at Black Rapids Glacier, Alaska. The probe is designed to operate at temperatures from 40 to +40 C and to withstand the cold, wet, high-pressure [130-atm (13.20-MPa)] environment at the bottom of a water-filled borehole in ice as deep as 1.6 km. A current version is being outfitted to service 2.4-km-deep boreholes at the Rutford Ice Stream in West Antarctica. The probe (see figure) contains a sidelooking charge-coupled-device (CCD) camera that generates both a real-time analog video signal and a sequence of still-image data, and contains a digital videotape recorder. The probe also contains a downward-looking CCD analog video camera, plus halogen lamps to illuminate the fields of view of both cameras. The analog video outputs of the cameras are converted to optical signals that are transmitted to a surface station via optical fibers in a cable. Electric power is supplied to the probe through wires in the cable at a potential of 170 VDC. A DC-to-DC converter steps the supply down to 12 VDC for the lights, cameras, and image-data-transmission circuitry. Heat generated by dissipation of electric power in the probe is removed simply by conduction through the probe housing to the visible features of the lower structure of the ice sheet, including ice layers formed at different times, bubbles, and mineralogical inclusions. At the time of reporting the information for this article, the system was just deployed in two boreholes on the Amery ice shelf in East Antarctica and after successful 2000 2001 deployments in 4 boreholes at Ice Stream C, West Antarctica, and in 2002 at Black Rapids Glacier, Alaska. The probe is designed to operate at temperatures from 40 to +40 C and to withstand the cold, wet, high-pressure [130-atm (13.20-MPa)] environment at the bottom of a water-filled borehole in ice as deep as 1.6 km. A current version is being outfitted to service 2.4-km-deep boreholes at the Rutford Ice Stream in West Antarctica. The probe (see figure) contains a sidelooking charge-coupled-device (CCD) camera that generates both a real-time analog video signal and a sequence of still-image data, and contains a digital videotape recorder. The probe also contains a downward-looking CCD analog video camera, plus halogen lamps to illuminate the fields of view of both cameras. The analog video outputs of the cameras are converted to optical signals that are transmitted to a surface station via optical fibers in a cable. Electric power is supplied to the probe through wires in the cable at a potential of 170 VDC. A DC-to-DC converter steps the supply down to 12 VDC for the lights, cameras, and image-datatransmission circuitry. Heat generated by dissipation of electric power in the probe is removed simply by conduction through the probe housing to the visible features of the lower structure of the ice sheet, including ice layers formed at different times, bubbles, and mineralogical inclusions. At thime of reporting the information for this article, the system was just deployed in two boreholes on the Amery ice shelf in East Antarctica and after successful 2000 2001 deployments in 4 boreholes at Ice Stream C, West Antarctica, and in 2002 at Black Rapids Glacier, Alaska. The probe is designed to operate at temperatures from 40 to +40 C and to withstand the cold, wet, high-pressure [130-atm (13.20-MPa)] environment at the bottom of a water-filled borehole in ice as deep as 1.6 km. A current version is being outfitted to service 2.4-km-deep boreholes at the Rutford Ice Stream in West Antarctica. The probe (see figure) contains a sidelooking charge-coupled-device (CCD) camera that generates both a real-time analog video signal and a sequence of still-image data, and contains a digital videotape recorder. The probe also contains a downward-looking CCD analog video camera, plus halogen lamps to illuminate the fields of view of both cameras. The analog video outputs of the cameras are converted to optical signals that are transmitted to a surface station via optical fibers in a cable. Electric power is supplied to the probe through wires in the cable at a potential of 170 VDC. A DC-to-DC converter steps the supply down to 12 VDC for the lights, cameras, and image-datatransmission circuitry. Heat generated by dissipation of electric power in the probe is removed simply by conduction through the probe housing to the adjacent water and ice.

Long-life mission reliability for outer planet atmospheric entry probes

NASA Technical Reports Server (NTRS)

Mccall, M. T.; Rouch, L.; Maycock, J. N.

1976-01-01

The results of a literature analysis on the effects of prolonged exposure to deep space environment on the properties of outer planet atmospheric entry probe components are presented. Materials considered included elastomers and plastics, pyrotechnic devices, thermal control components, metal springs and electronic components. The rates of degradation of each component were determined and extrapolation techniques were used to predict the effects of exposure for up to eight years to deep space. Pyrotechnic devices were aged under accelerated conditions to an equivalent of eight years in space and functionally tested. Results of the literature analysis of the selected components and testing of the devices indicated that no severe degradation should be expected during an eight year space mission.

Seismic detectability of meteorite impacts on Europa

NASA Astrophysics Data System (ADS)

Tsuji, Daisuke; Teanby, Nicholas

2016-04-01

Europa, the second of Jupiter's Galilean satellites, has an icy outer shell, beneath which there is probably liquid water in contact with a rocky core. Europa, may thus provide an example of a sub-surface habitable environment so is an attractive object for future lander missions. In fact, the Jupiter Icy Moon Explorer (JUICE) mission has been selected for the L1 launch slot of ESA's Cosmic Vision science programme with the aim of launching in 2022 to explore Jupiter and its potentially habitable icy moons. One of the best ways to probe icy moon interiors in any future mission will be with a seismic investigation. Previously, the Apollo seismic experiment, installed by astronauts, enhanced our knowledge of the lunar interior. For a recent mission, NASA's 2016 InSight Mars lander aims to obtain seismic data and will deploy a seismometer directly onto Mars' surface. Motivated by these works, in this study we show how many meteorite impacts will be detected using a single seismic station on Europa, which will be useful for planning the next generation of outer solar system missions. To this end, we derive: (1) the current small impact flux on Europa from Jupiter impact rate models; (2) a crater diameter versus impactor energy scaling relation for ice by merging previous experiments and simulations; (3) scaling relations for seismic signals as a function of distance from an impact site for a given crater size based on analogue explosive data obtained on Earth's icy surfaces. Finally, resultant amplitudes are compared to the noise level of a likely seismic instrument (based on the NASA InSight mission seismometers) and the number of detectable impacts are estimated. As a result, 0.5-3.0 local/regional small impacts (i.e., direct P-waves through the ice crust) are expected to be detected per year, while global-scale impact events (i.e., PKP-waves refracted through the mantle) are rare and unlikely to be detected by a short duration mission. We note that our results are only appropriate for order of magnitude calculations because of considerable uncertainties in the small impactor source population, internal structure, and ambient noise level. However, our results suggest that probing the deep interior using impacts will be challenging and require an extended mission duration and low noise levels to give a reasonable chance of detection. Therefore, for future seismic exploration, faulting due to stresses in the rigid outer ice shell is likely to be much more viable mechanism for probing the interior.

Impact of Near-Earth Plasma Sheet Dynamics on the Ring Current Composition

NASA Astrophysics Data System (ADS)

Kistler, L. M.; Mouikis, C.; Menz, A.; Spence, H. E.; Mitchell, D. G.; Gkioulidou, M.; Lanzerotti, L. J.; Skoug, R. M.; Larsen, B.; Claudepierre, S. G.; Fennell, J. F.; Blake, J. B.

2014-12-01

How the dynamics in the near-earth plasma sheet affects the heavy ion content, and therefore the ion pressure, of the ring current in Earth's magnetosphere is an outstanding question. Substorms accelerate plasma in the near-earth region and drive outflow from the aurora, and both these processes can preferentially enhance the population of heavy ions in this region. These heavy ions are then driven into the inner magnetosphere during storms. Thus understanding how the composition of the ring current changes requires simultaneous observations in the near-earth plasma sheet and in the inner magnetosphere. We use data from the CODIF instrument on Cluster and HOPE, RBSPICE, and MagEIS instruments on the Van Allen Probes to study the acceleration and transport of ions from the plasma sheet into the ring current. During the main phase of a geomagnetic storm on Aug 4-6, 2013, the Cluster spacecraft were moving inbound in the midnight central plasma sheet, while the apogees of the two Van Allen Probes were located on the duskside. The Cluster spacecraft measure the composition and spectral changes in the plasma sheet, while the Van Allen Probes measure the ions that reach the inner magnetosphere. A strong increase in 1-40 keV O+ was observed at the Cluster location during the storm main phase, and the Van Allen Probes observed both H+ and O+ being driven deep into the inner magnetosphere. By comparing the variations in phase space density (PSD) vs. magnetic moment at the Cluster and the Van Allen Probes locations, we examine how the composition changes non-adiabatically in the near-earth plasma sheet, and how those changes are propagated into the inner magnetosphere, populating the hto ion ring current.

Flexible poly(methyl methacrylate)-based neural probe: An affordable implementation

NASA Astrophysics Data System (ADS)

Gasemi, Pejman; Veladi, Hadi; Shahabi, Parviz; Khalilzadeh, Emad

2018-03-01

This research presents a novel technique used to fabricate a deep brain stimulation probe based on a commercial poly(methyl methacrylate) (PMMA) polymer. This technique is developed to overcome the high cost of available probes crucial for chronic stimulation and recording in neural disorders such as Parkinson’s disease and epilepsy. The probe is made of PMMA and its mechanical properties have been customized by controlling the reaction conditions. The polymer is adjusted to be stiff enough to be easily inserted and, on the other hand, soft enough to perform required movements. As cost is one of the issues in the use of neural probes, a simple process is proposed for the production of PMMA neural probes without using expensive equipment and operations, and without compromising performance and quality. An in vivo animal test was conducted to observe the recording capability of a PMMA probe.

Prompt Acceleration of Magnetospheric Electrons to Ultrarelativistic Energies by the 17 March 2015 Interplanetary Shock

NASA Technical Reports Server (NTRS)

Kanekal, S. G.; Baker, D. N.; Fennell, J. F.; Jones, A.; Schiller, Q.; Richardson, I.G.; Li, X.; Turner, D. L.; Califf, S.; Claudepierre, S. G.;

Comments on "J. Vera et. al., Soil water balance trial involving capacitance and neutron probe measurements"

USDA-ARS?s Scientific Manuscript database

Vera et al. (2009) compared estimates of soil profile water content (mm) to a depth of 0.8 m made with the neutron moisture meter (NMM) and a multi-depth capacitance probe (MDCP), using measurements replicated in four drainage lysimeters (5 m x 5 m x 1.5-m deep). The NMM estimates of water content w...

[Impact of volatile sulphur compounds in periodontal pockets on initial periodontal therapy].

PubMed

Li, Xiao-Jun; Dong, Ling-Ling; Kong, Jing-Jia

2008-07-01

To investigate the relationship of volatile sulphur compounds((VSC)levels in periodontal pockets with severity of periodontitis, and the impact of VSC on the result of initial periodontal therapy. Twenty-five patients with chronic periodontitis(CP)(13 males and 12 females with average age of 35) were included in this study. Clinical periodontal parameters, plaque index, probing depth(PD), attachment loss(AL), and bleeding on probing(BOP) were recorded before and 3 months after the initial therapy. VSC levels were measured with a portable monitor in a digital score ranging from 0.0 to 5.0. All of 5 054 sites for 840 teeth were included in this study. Before treatment the percentage of VSC-positive sites was 17.1%, 52.3% and 86.0% for shallow (PD<3 mm), moderate(PD 4-6 mm) and deep (PD>7 mm) pocket, respectively (P<0.001). In most VSC-positive sites the VSC levels were<1.0. Percentage of sites with a high VSC levels was significantly different among three groups (P<0.01). All clinical parameters in VSC-negative sites were reduced significantly following the initial therapy. The reduction of PD and AL in VSC-positive sites by treatment was less marked than that in VSC-negative sites. VSC in periodontal pockets may be a potential indicator for detecting severity of CP and a useful predictor for therapeutic success.

Making the Venus Concept Watch 1.0

NASA Astrophysics Data System (ADS)

Balint, Tibor S.; Melchiorri, Julian P.

2014-08-01

Over the past year we have celebrated the 50th anniversary of planetary exploration, which started with the Venus flyby of Mariner-2; and the 35th anniversary of the Pioneer-Venus multi-probe mission where one large and three small probes descended to the surface of Venus, encountering extreme environmental conditions. At the surface of Venus the temperature is about 460 °C, and the pressure is 92 bar, with a highly corrosive super-critical CO2 atmosphere. At a Venusian altitude of 50 km the pressure and temperature conditions are near Earth-like, but the clouds carry sulfuric acid droplets. Deep probe missions to Jupiter and Saturn, targeting the 100 bar pressure depth encounter similar pressure and temperature conditions as the Pioneer-Venus probes did. Mitigating these environments is highly challenging and requires special considerations for designs and materials. While assessing such space mission concepts, we have found that there is an overlap between the extreme environments in planetary atmospheres and the environments experienced by deep-sea explorers back on Earth. Consequently, the mitigation approaches could be also similar between planetary probes and diver watches. For example, both need to tolerate about 100 bar of pressure-although high temperatures are not factors on Earth. Mitigating these environments, the potential materials are: titanium for the probe and the watch housing; sapphire for the window and glass; resin impregnated woven carbon fiber for the aeroshell's thermal protection system and for the face of the watch; and nylon ribbon for the parachute and for the watch band. Planetary probes also utilize precision watches; thus there is yet another crosscutting functionality with diver watches. Our team, from the Innovation Design Engineering Program of the Royal College of Art, has designed and built a concept watch to commemorate these historical events, while highlighting advances in manufacturing processes over the past three to five decades, relevant to both future planetary mission designs and can be used to produce deep diver watches. In this paper we describe our design considerations; give a brief overview of the extreme environments these components would experience on both Venus and Earth; the manufacturing techniques and materials we used to build the Venus Watch; and its outreach potential to bring a distant concept of planetary exploration closer to Earth. We will also address lessons learned from this project and new ideas forward, for the next generation of this concept design.

How does mesoscale impact deep convection? Answers from ensemble Northwestern Mediterranean Sea simulations.

NASA Astrophysics Data System (ADS)

Waldman, Robin; Herrmann, Marine; Somot, Samuel; Arsouze, Thomas; Benshila, Rachid; Bosse, Anthony; Chanut, Jérôme; Giordani, Hervé; Pennel, Romain; Sevault, Florence; Testor, Pierre

2017-04-01

Ocean deep convection is a major process of interaction between surface and deep ocean. The Gulf of Lions is a well-documented deep convection area in the Mediterranean Sea, and mesoscale dynamics is a known factor impacting this phenomenon. However, previous modelling studies don't allow to address the robustness of its impact with respect to the physical configuration and ocean intrinsic variability. In this study, the impact of mesoscale on ocean deep convection in the Gulf of Lions is investigated using a multi-resolution ensemble simulation of the northwestern Mediterranean sea. The eddy-permitting Mediterranean model NEMOMED12 (6km resolution) is compared to its eddy-resolving counterpart with the 2-way grid refinement AGRIF in the northwestern Mediterranean (2km resolution). We focus on the well-documented 2012-2013 period and on the multidecadal timescale (1979-2013). The impact of mesoscale on deep convection is addressed in terms of its mean and variability, its impact on deep water transformations and on associated dynamical structures. Results are interpreted by diagnosing regional mean and eddy circulation and using buoyancy budgets. We find a mean inhibition of deep convection by mesoscale with large interannual variability. It is associated with a large impact on mean and transient circulation and a large air-sea flux feedback.

Recalcitrant deep and shallow nodes in Aristolochia (Aristolochiaceae) illuminated using anchored hybrid enrichment.

PubMed

Wanke, Stefan; Granados Mendoza, Carolina; Müller, Sebastian; Paizanni Guillén, Anna; Neinhuis, Christoph; Lemmon, Alan R; Lemmon, Emily Moriarty; Samain, Marie-Stéphanie

2017-12-01

Recalcitrant relationships are characterized by very short internodes that can be found among shallow and deep phylogenetic scales all over the tree of life. Adding large amounts of presumably informative sequences, while decreasing systematic error, has been suggested as a possible approach to increase phylogenetic resolution. The development of enrichment strategies, coupled with next generation sequencing, resulted in a cost-effective way to facilitate the reconstruction of recalcitrant relationships. By applying the anchored hybrid enrichment (AHE) genome partitioning strategy to Aristolochia using an universal angiosperm probe set, we obtained 231-233 out of 517 single or low copy nuclear loci originally contained in the enrichment kit, resulting in a total alignment length of 154,756bp to 160,150bp. Since Aristolochia (Piperales; magnoliids) is distantly related to any angiosperm species whose genome has been used for the plant AHE probe design (Amborella trichopoda being the closest), it serves as a proof of universality for this probe set. Aristolochia comprises approximately 500 species grouped in several clades (OTUs), whose relationships to each other are partially unknown. Previous phylogenetic studies have shown that these lineages branched deep in time and in quick succession, seen as short-deep internodes. Short-shallow internodes are also characteristic of some Aristolochia lineages such as Aristolochia subsection Pentandrae, a clade of presumably recent diversification. This subsection is here included to test the performance of AHE at species level. Filtering and subsampling loci using the phylogenetic informativeness method resolves several recalcitrant phylogenetic relationships within Aristolochia. By assuming different ploidy levels during bioinformatics processing of raw data, first hints are obtained that polyploidization contributed to the evolution of Aristolochia. Phylogenetic results are discussed in the light of current systematics and morphology. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

Oral Microbiome of Deep and Shallow Dental Pockets In Chronic Periodontitis

PubMed Central

Ge, Xiuchun; Rodriguez, Rafael; Trinh, My; Gunsolley, John; Xu, Ping

2013-01-01

We examined the subgingival bacterial biodiversity in untreated chronic periodontitis patients by sequencing 16S rRNA genes. The primary purpose of the study was to compare the oral microbiome in deep (diseased) and shallow (healthy) sites. A secondary purpose was to evaluate the influences of smoking, race and dental caries on this relationship. A total of 88 subjects from two clinics were recruited. Paired subgingival plaque samples were taken from each subject, one from a probing site depth >5 mm (deep site) and the other from a probing site depth ≤3mm (shallow site). A universal primer set was designed to amplify the V4–V6 region for oral microbial 16S rRNA sequences. Differences in genera and species attributable to deep and shallow sites were determined by statistical analysis using a two-part model and false discovery rate. Fifty-one of 170 genera and 200 of 746 species were found significantly different in abundances between shallow and deep sites. Besides previously identified periodontal disease-associated bacterial species, additional species were found markedly changed in diseased sites. Cluster analysis revealed that the microbiome difference between deep and shallow sites was influenced by patient-level effects such as clinic location, race and smoking. The differences between clinic locations may be influenced by racial distribution, in that all of the African Americans subjects were seen at the same clinic. Our results suggested that there were influences from the microbiome for caries and periodontal disease and these influences are independent. PMID:23762384

Innovations in Sampling Pore Fluids From Deep-Sea Hydrate Sites

NASA Astrophysics Data System (ADS)

Lapham, L. L.; Chanton, J. P.; Martens, C. S.; Schaefer, H.; Chapman, N. R.; Pohlman, J. W.

2003-12-01

We have developed a sea-floor probe capable of collecting and returning undecompressed pore water samples at in situ pressures for determination of dissolved gas concentrations and isotopic values in deep-sea sediments. In the summer of 2003, we tested this instrument in sediments containing gas hydrates off Vancouver Island, Cascadia Margin from ROPOS (a remotely operated vehicle) and in the Gulf of Mexico from Johnson-Sea-Link I (a manned submersible). Sediment push cores were collected alongside the probe to compare methane concentrations and stable carbon isotope compositions in decompressed samples vs. in situ samples obtained by probe. When sufficient gas was available, ethane and propane concentrations and isotopes were also compared. Preliminary data show maximum concentrations of dissolved methane to be 5mM at the Cascadia Margin Fish Boat site (850m water depth) and 12mM in the Gulf of Mexico Bush Hill hydrate site (550m water depth). Methane concentrations were, on average, five times as high in probe samples as in the cores. Carbon isotopic values show a thermogenic input and oxidative effects approaching the sediment-water interface at both sites. This novel data set will provide information that is critical to the understanding of the in situ processes and environmental conditions controlling gas hydrate occurrences in sediments.

Oceanic Basement Probed

ERIC Educational Resources Information Center

Cann, J. R.; Moore, David G.

1978-01-01

Summarizes findings of the deep sea drilling project at Scripps Institute of Oceanology. Results of Atlantic and Pacific Ocean drillings in terms of the composition and properties of the sea floor are discussed. (CP)

Van Allen Probes Measurements of Energetic Particle Deep Penetration Into the Low L Region (L < 4) During the Storm on 8 April 2016

NASA Astrophysics Data System (ADS)

Zhao, H.; Baker, D. N.; Califf, S.; Li, X.; Jaynes, A. N.; Leonard, T.; Kanekal, S. G.; Blake, J. B.; Fennell, J. F.; Claudepierre, S. G.; Turner, D. L.; Reeves, G. D.; Spence, H. E.

2017-12-01

Using measurements from the Van Allen Probes, a penetration event of tens to hundreds of keV electrons and tens of keV protons into the low L shells (L < 4) is studied. Timing and magnetic local time (MLT) differences of energetic particle deep penetration are unveiled and underlying physical processes are examined. During this event, both proton and electron penetrations are MLT asymmetric. The observed MLT difference of proton penetration is consistent with convection of plasma sheet protons, suggesting enhanced convection during geomagnetic active times to be the cause of energetic proton deep penetration during this event. The observed MLT difference of tens to hundreds of keV electron penetration is completely different from tens of keV protons and cannot be well explained by inward radial diffusion, convection of plasma sheet electrons, or transport of trapped electrons by enhanced convection electric field represented by the Volland-Stern model or a uniform dawn-dusk electric field model based on the electric field measurements. It suggests that the underlying physical mechanism responsible for energetic electron deep penetration, which is very important for fully understanding energetic electron dynamics in the low L shells, should be MLT localized.

Plume and Discharge Plasma Measurements of an NSTAR-type Ion Thruster

NASA Technical Reports Server (NTRS)

Foster, John E.; Soulas, George C.; Patterson, Michael J.

2000-01-01

The success of the NASA Deep Space 1 spacecraft has demonstrated that ion propulsion is a viable option for deep space science missions. More aggressive missions such as Comet Nuclear Sample Return and Europa lander will require higher power, higher propellant throughput and longer thruster lifetime than the NASA Solar Electric Propulsion Technology Application Readiness (NSTAR) engine. Presented here are thruster plume and discharge plasma measurements of an NSTAR-type thruster operated from 0.5 kW to 5 kW. From Faraday plume sweeps, beam divergence was determined. From Langmuir probe plume measurements on centerline, low energy ion production on axis due to charge-exchange and direct ionization was assessed. Additionally, plume plasma potential measurements made on axis were used to determine the upper energy limits at which ions created on centerline could be radially accelerated. Wall probes flush-mounted to the thruster discharge chamber anode were used to assess plasma conditions. Langmuir probe measurements at the wall indicated significant differences in the electron temperature in the cylindrical and conical sections of the discharge chamber.

Plume and Discharge Plasma Measurements of an NSTAR-type Ion Thruster

NASA Technical Reports Server (NTRS)

Foster, John E; Soulas, George C.; Patterson, Michael J.

2000-01-01

The success of the NASA Deep Space I spacecraft has demonstrated that ion propulsion is a viable option for deep space science missions. More aggressive missions such as Comet Nuclear Sample Return and Europa lander will require higher power, higher propellant throughput and longer thruster lifetime than the NASA Solar Electric Propulsion Technology Application Readiness (NSTAR) engine. Presented here are thruster plume and discharge plasma measurements of an NSTAR-type thruster operated from 0.5 kW to 5 kW. From Faraday plume sweeps, beam divergence was determined. From Langmuir probe plume measurements on centerline, low energy ion production on axis due to charge-exchange and direct ionization was assessed. Additionally, plume plasma potential measurements made on axis were used to determine the upper energy limits at which ions created on centerline could be radially accelerated. Wall probes flush-mounted to the thruster discharge chamber anode were used to assess plasma conditions. Langmuir probe measurements at the wall indicated significant differences in the electron temperature in the cylindrical and conical sections of the discharge chamber.

Transurethral illumination probe design for deep photoacoustic imaging of prostate

NASA Astrophysics Data System (ADS)

Ai, Min; Salcudean, Tim; Rohling, Robert; Abolmaesumi, Purang; Tang, Shuo

2018-02-01

Photoacoustic (PA) imaging with internal light illumination through optical fiber could enable imaging of internal organs at deep penetration. We have developed a transurethral probe with a multimode fiber inserted in a rigid cystoscope sheath for illuminating the prostate. At the distal end, the fiber tip is processed to diffuse light circumferentially over 2 cm length. A parabolic cylinder mirror then reflects the light to form a rectangular-shaped parallel beam which has at least 1 cm2 at the probe surface. The relatively large rectangular beam size can reduce the laser fluence rate on the urethral wall and thus reduce the potential of tissue damage. A 3 cm optical penetration in chicken tissue is achieved at a fluence rate around 7 mJ/cm2 . For further validation, a prostate phantom was built with similar optical properties of the human prostate. A 1.5 cm penetration depth is achieved in the prostate mimicking phantom at 10 mJ/cm2 fluence rate. PA imaging of prostate can potentially be carried out in the future by combining a transrectal ultrasound transducer and the transurethral illumination.

Atmospheric entry probes for outer planet exploration. Outer planet entry probe technical summary

NASA Technical Reports Server (NTRS)

1974-01-01

The use of unmanned space probes for investigating the conditions existing on and around the outer planets of the solar system is discussed. The subjects included in the report are: (1) the design of a common entry probe for outer planet missions, (2) the significant trades related to the development of a common probe design, (3) the impact of bus selection on probe design, (4) the impact of probe requirements on bus modifications, and (5) the key technology elements recommended for advanced development. Drawings and illustrations of typical probes are included to show the components and systems used in the space probes.

49 CFR 572.134 - Thorax assembly and test procedure.

Code of Federal Regulations, 2013 CFR

2013-10-01

... completely assembled dummy (drawing 880105-000) is impacted by a test probe conforming to section 572.137(a...). Within this specified compression corridor, the peak force, measured by the impact probe as defined in... midsagittal plane so that the impact point of the longitudinal centerline of the probe coincides with the...

49 CFR 572.134 - Thorax assembly and test procedure.

Code of Federal Regulations, 2011 CFR

2011-10-01

... completely assembled dummy (drawing 880105-000) is impacted by a test probe conforming to section 572.137(a...). Within this specified compression corridor, the peak force, measured by the impact probe as defined in... midsagittal plane so that the impact point of the longitudinal centerline of the probe coincides with the...

49 CFR 572.10 - Limbs.

Code of Federal Regulations, 2011 CFR

2011-10-01

... of the forward edge of the seat. (3) Align the test probe specified in § 572.11(a) so that at impact...) Impact the knee with the test probe moving horizontally and parallel to the midsagittal plane at the specified velocity. (5) Guide the probe during impact so that it moves with no significant lateral, vertical...

49 CFR 572.134 - Thorax assembly and test procedure.

Code of Federal Regulations, 2012 CFR

2012-10-01

... completely assembled dummy (drawing 880105-000) is impacted by a test probe conforming to section 572.137(a...). Within this specified compression corridor, the peak force, measured by the impact probe as defined in... midsagittal plane so that the impact point of the longitudinal centerline of the probe coincides with the...

49 CFR 572.10 - Limbs.

Code of Federal Regulations, 2014 CFR

2014-10-01

... of the forward edge of the seat. (3) Align the test probe specified in § 572.11(a) so that at impact...) Impact the knee with the test probe moving horizontally and parallel to the midsagittal plane at the specified velocity. (5) Guide the probe during impact so that it moves with no significant lateral, vertical...

49 CFR 572.10 - Limbs.

Code of Federal Regulations, 2012 CFR

2012-10-01

... of the forward edge of the seat. (3) Align the test probe specified in § 572.11(a) so that at impact...) Impact the knee with the test probe moving horizontally and parallel to the midsagittal plane at the specified velocity. (5) Guide the probe during impact so that it moves with no significant lateral, vertical...

49 CFR 572.10 - Limbs.

Code of Federal Regulations, 2013 CFR

2013-10-01

... of the forward edge of the seat. (3) Align the test probe specified in § 572.11(a) so that at impact...) Impact the knee with the test probe moving horizontally and parallel to the midsagittal plane at the specified velocity. (5) Guide the probe during impact so that it moves with no significant lateral, vertical...

49 CFR 572.174 - Thorax assembly and test procedure.

Code of Federal Regulations, 2012 CFR

2012-10-01

... assembled dummy (drawing 420-0000) (incorporated by reference, see § 572.170) is impacted by a test probe... this specified compression corridor, the peak force, measured by the impact probe as defined in section... midsagittal plane so that the impact point of the longitudinal centerline of the probe coincides with the...

49 CFR 572.134 - Thorax assembly and test procedure.

Code of Federal Regulations, 2014 CFR

2014-10-01

... completely assembled dummy (drawing 880105-000) is impacted by a test probe conforming to section 572.137(a...). Within this specified compression corridor, the peak force, measured by the impact probe as defined in... midsagittal plane so that the impact point of the longitudinal centerline of the probe coincides with the...

49 CFR 572.174 - Thorax assembly and test procedure.

Code of Federal Regulations, 2014 CFR

2014-10-01

... assembled dummy (drawing 420-0000) (incorporated by reference, see § 572.170) is impacted by a test probe... this specified compression corridor, the peak force, measured by the impact probe as defined in section... midsagittal plane so that the impact point of the longitudinal centerline of the probe coincides with the...

49 CFR 572.174 - Thorax assembly and test procedure.

Code of Federal Regulations, 2013 CFR

2013-10-01

... assembled dummy (drawing 420-0000) (incorporated by reference, see § 572.170) is impacted by a test probe... this specified compression corridor, the peak force, measured by the impact probe as defined in section... midsagittal plane so that the impact point of the longitudinal centerline of the probe coincides with the...

Probing carbon impurities in hexagonal boron nitride epilayers

NASA Astrophysics Data System (ADS)

Uddin, M. R.; Li, J.; Lin, J. Y.; Jiang, H. X.

2017-05-01

Carbon doped hexagonal boron nitride epilayers have been grown by metal organic chemical vapor deposition. Photocurrent excitation spectroscopy has been utilized to probe the energy levels associated with carbon impurities in hexagonal boron nitride (h-BN). The observed transition peaks in photocurrent excitation spectra correspond well to the energy positions of the bandgap, substitutional donors (CB, carbon impurities occupying boron sites), and substitutional acceptors (CN, carbon impurities occupying nitrogen sites). From the observed transition peak positions, the derived energy level of CB donors in h-BN is ED ˜ 0.45 eV, which agrees well with the value deduced from the temperature dependent electrical resistivity. The present study further confirms that the room temperature bandgap of h-BN is about 6.42-6.45 eV, and the CN deep acceptors have an energy level of about 2.2-2.3 eV. The results also infer that carbon doping introduces both shallow donors (CB) and deep acceptors (CN) via self-compensation, and the energy level of carbon donors appears to be too deep to enable carbon as a viable candidate as an n-type dopant in h-BN epilayers.

A bright cyan-excitable orange fluorescent protein facilitates dual-emission microscopy and enhances bioluminescence imaging in vivo

PubMed Central

Chu, Jun; Oh, Young-Hee; Sens, Alex; Ataie, Niloufar; Dana, Hod; Macklin, John J.; Laviv, Tal; Welf, Erik S.; Dean, Kevin M.; Zhang, Feijie; Kim, Benjamin B.; Tang, Clement Tran; Hu, Michelle; Baird, Michelle A.; Davidson, Michael W.; Kay, Mark A.; Fiolka, Reto; Yasuda, Ryohei; Kim, Douglas S.; Ng, Ho-Leung; Lin, Michael Z.

2016-01-01

Orange-red fluorescent proteins (FPs) are widely used in biomedical research for multiplexed epifluorescence microscopy with GFP-based probes, but their different excitation requirements make multiplexing with new advanced microscopy methods difficult. Separately, orange-red FPs are useful for deep-tissue imaging in mammals due to the relative tissue transmissibility of orange-red light, but their dependence on illumination limits their sensitivity as reporters in deep tissues. Here we describe CyOFP1, a bright engineered orange-red FP that is excitable by cyan light. We show that CyOFP1 enables single-excitation multiplexed imaging with GFP-based probes in single-photon and two-photon microscopy, including time-lapse imaging in light-sheet systems. CyOFP1 also serves as an efficient acceptor for resonance energy transfer from the highly catalytic blue-emitting luciferase NanoLuc. An optimized fusion of CyOFP1 and NanoLuc, called Antares, functions as a highly sensitive bioluminescent reporter in vivo, producing substantially brighter signals from deep tissues than firefly luciferase and other bioluminescent proteins. PMID:27240196

Deep-water longline fishing has reduced impact on Vulnerable Marine Ecosystems

PubMed Central

Pham, Christopher K.; Diogo, Hugo; Menezes, Gui; Porteiro, Filipe; Braga-Henriques, Andreia; Vandeperre, Frederic; Morato, Telmo

2014-01-01

Bottom trawl fishing threatens deep-sea ecosystems, modifying the seafloor morphology and its physical properties, with dramatic consequences on benthic communities. Therefore, the future of deep-sea fishing relies on alternative techniques that maintain the health of deep-sea ecosystems and tolerate appropriate human uses of the marine environment. In this study, we demonstrate that deep-sea bottom longline fishing has little impact on vulnerable marine ecosystems, reducing bycatch of cold-water corals and limiting additional damage to benthic communities. We found that slow-growing vulnerable species are still common in areas subject to more than 20 years of longlining activity and estimate that one deep-sea bottom trawl will have a similar impact to 296–1,719 longlines, depending on the morphological complexity of the impacted species. Given the pronounced differences in the magnitude of disturbances coupled with its selectivity and low fuel consumption, we suggest that regulated deep-sea longlining can be an alternative to deep-sea bottom trawling. PMID:24776718

Slantwise convection on fluid planets: Interpreting convective adjustment from Juno observations

NASA Astrophysics Data System (ADS)

O'Neill, M. E.; Kaspi, Y.; Galanti, E.

2016-12-01

NASA's Juno mission provides unprecedented microwave measurements that pierce Jupiter's weather layer and image the transition to an adiabatic fluid below. This region is expected to be highly turbulent and complex, but to date most models use the moist-to-dry transition as a simple boundary. We present simple theoretical arguments and GCM results to argue that columnar convection is important even in the relatively thin boundary layer, particularly in the equatorial region. We first demonstrate how surface cooling can lead to very horizontal parcel paths, using a simple parcel model. Next we show the impact of this horizontal motion on angular momentum flux in a high-resolution Jovian model. The GCM is a state-of-the-art modification of the MITgcm, with deep geometry, compressibility and interactive two-stream radiation. We show that slantwise convection primarily mixes fluid along columnar surfaces of angular momentum, and discuss the impacts this should have on lapse rate interpretation of both the Galileo probe sounding and the Juno microwave observations.

EXTASE - An Experimental Thermal Probe for Applications in Snow Research and Earth Sciences

NASA Astrophysics Data System (ADS)

Schroeer, K.; Seiferlin, K.; Marczewski, W.; Gadomski, S.; Spohn, T.

2002-12-01

EXTASE is a spin-off project from the Rosetta Lander (MUPUS) thermal probe, funded by DLR. The application of this probe is to be tested in different fields, e.g. in snow research, agriculture, permafrost etc. The system consists of the probe itself with a portable field electronic and a computer for control of the system and storage of the data. The probe penetrates the surface ca. 32 cm deep and provides a temperature profile (16 sensors) and thermal conductivity profile of the penetrated layer. The main advantages of the probe in comparison to common temperature profile measurement methods are: - no need to excavate material - minimized influence of the probe on the temperature field - minimized modification of the microstructure of the studied medium. Presently we are concentrating on agriculture (soil humidity) and snow research. Further applications could be e.g.: monitoring waste deposits and the heat released by decomposition, volcanology and ground truth for remote sensing. We present the general concept of the probe and also data obtained during different field measurement campaigns with prototypes of the probe.

Development of a new method for the noninvasive measurement of deep body temperature without a heater.

PubMed

Kitamura, Kei-Ichiro; Zhu, Xin; Chen, Wenxi; Nemoto, Tetsu

2010-01-01

The conventional zero-heat-flow thermometer, which measures the deep body temperature from the skin surface, is widely used at present. However, this thermometer requires considerable electricity to power the electric heater that compensates for heat loss from the probe; thus, AC power is indispensable for its use. Therefore, this conventional thermometer is inconvenient for unconstrained monitoring. We have developed a new dual-heat-flux method that can measure the deep body temperature from the skin surface without a heater. Our method is convenient for unconstrained and long-term measurement because the instrument is driven by a battery and its design promotes energy conservation. Its probe consists of dual-heat-flow channels with different thermal resistances, and each heat-flow-channel has a pair of IC sensors attached on its top and bottom. The average deep body temperature measurements taken using both the dual-heat-flux and then the zero-heat-flow thermometers from the foreheads of 17 healthy subjects were 37.08 degrees C and 37.02 degrees C, respectively. In addition, the correlation coefficient between the values obtained by the 2 methods was 0.970 (p<0.001). These results show that our method can be used for monitoring the deep body temperature as accurately as the conventional method, and it overcomes the disadvantage of the necessity of AC power supply. (c) 2009 IPEM. Published by Elsevier Ltd. All rights reserved.

Big, Deep, and Smart Data in Scanning Probe Microscopy

DOE PAGES

Kalinin, Sergei V.; Strelcov, Evgheni; Belianinov, Alex; ...

2016-09-27

Scanning probe microscopy techniques open the door to nanoscience and nanotechnology by enabling imaging and manipulation of structure and functionality of matter on nanometer and atomic scales. We analyze the discovery process by SPM in terms of information flow from tip-surface junction to the knowledge adoption by scientific community. Furthermore, we discuss the challenges and opportunities offered by merging of SPM and advanced data mining, visual analytics, and knowledge discovery technologies.

Sealing scientific probes against deep space and the Venusian environment A tough job

NASA Technical Reports Server (NTRS)

Pokras, J.; Reinert, R. P.; Switz, R. J.

1978-01-01

The Pioneer Venus mission evolved from studies conducted during the late 1960s and early 1970s. It was found that a need existed for low cost orbiters and landers to explore the planet. The considered mission was to be accomplished with six separate vehicles arriving at Venus nearly simultaneously in mid-December 1978. The probes are designed to survive entry and descent into the atmosphere. A description is presented of the approaches used to maintain sealing integrity for the large and small probes under the constraints imposed by the harsh Venusian environment. Attention is given to probe vehicle configuration, pressure vessel sealing requirements, material and configuration considerations, permanent seals, separable seals, development problems, and aspects of seal testing.

Probe-pin device for optical neurotransmitter sensing in the brain

NASA Astrophysics Data System (ADS)

Kim, Min Hyuck; Song, Kyo D.; Yoon, Hargsoon; Park, Yeonjoon; Choi, Sang H.; Lee, Dae-Sung; Shin, Kyu-Sik; Hwang, Hak-In; Lee, Uhn

2015-04-01

Development of an optical neurotransmitter sensing device using nano-plasmonic probes and a micro-spectrometer for real time monitoring of neural signals in the brain is underway. Clinical application of this device technology is to provide autonomous closed-loop feedback control to a deep brain stimulation (DBS) system and enhance the accuracy and efficacy of DBS treatment. By far, we have developed an implantable probe-pin device based on localized field enhancement of surface plasmonic resonance on a nanostructured sensing domain which can amplify neurochemical signals from evoked neural activity in the brain. In this paper, we will introduce the details of design and sensing performance of a proto-typed microspectrometer and nanostructured probing devices for real time measurement of neurotransmitter concentrations.

Towards label-free and site-specific probing of the local pH in proteins: pH-dependent deep UV Raman spectra of histidine and tyrosine

NASA Astrophysics Data System (ADS)

Bröermann, Andreas; Steinhoff, Heinz-Jürgen; Schlücker, Sebastian

2014-09-01

The site-specific pH is an experimental probe for assessing models of structural folding and function of a protein as well as protein-protein and protein-ligand interactions. It can be determined by various techniques such as NMR, FT-IR, fluorescence and EPR spectroscopy. The latter require the use of external labels, i.e., employ pH-dependent dyes and spin labels, respectively. In this contribution, we outline an approach to a label-free and site-specific method for determining the local pH using deep ultraviolet resonance Raman (UVRR) spectroscopic fingerprints of the aromatic amino acids histidine and tyrosine in combination with a robust algorithm that determines the pH value using three UVRR reference spectra and without prior knowledge of the pKa.

Safe Laser Beam Propagation for Interplanetary Links

NASA Technical Reports Server (NTRS)

Wilson, Keith E.

2011-01-01

Ground-to-space laser uplinks to Earth–orbiting satellites and deep space probes serve both as a beacon and an uplink command channel for deep space probes and Earth-orbiting satellites. An acquisition and tracking point design to support a high bandwidth downlink from a 20-cm optical terminal on an orbiting Mars spacecraft typically calls for 2.5 kW of 1030-nm uplink optical power in 40 micro-radians divergent beams.2 The NOHD (nominal ocular hazard distance) of the 1030nm uplink is in excess of 2E5 km, approximately half the distance to the moon. Recognizing the possible threat of high power laser uplinks to the flying public and to sensitive Earth-orbiting satellites, JPL developed a three-tiered system at its Optical Communications Telescope Laboratory (OCTL) to ensure safe laser beam propagation through navigational and near-Earth space.

The Chandra Deep Field-North Survey and the cosmic X-ray background.

PubMed

Brandt, W Nielsen; Alexander, David M; Bauer, Franz E; Hornschemeier, Ann E

2002-09-15

Chandra has performed a 1.4 Ms survey centred on the Hubble Deep Field-North (HDF-N), probing the X-ray Universe 55-550 times deeper than was possible with pre-Chandra missions. We describe the detected point and extended X-ray sources and discuss their overall multi-wavelength (optical, infrared, submillimetre and radio) properties. Special attention is paid to the HDF-N X-ray sources, luminous infrared starburst galaxies, optically faint X-ray sources and high-to-extreme redshift active galactic nuclei. We also describe how stacking analyses have been used to probe the average X-ray-emission properties of normal and starburst galaxies at cosmologically interesting distances. Finally, we discuss plans to extend the survey and argue that a 5-10 Ms Chandra survey would lay key groundwork for future missions such as XEUS and Generation-X.

Could Nano-Structured Materials Enable the Improved Pressure Vessels for Deep Atmospheric Probes?

NASA Technical Reports Server (NTRS)

Srivastava, D.; Fuentes, A.; Bienstock, B.; Arnold, J. O.

2005-01-01

A viewgraph presentation on the use of Nano-Structured Materials to enable pressure vessel structures for deep atmospheric probes is shown. The topics include: 1) High Temperature/Pressure in Key X-Environments; 2) The Case for Use of Nano-Structured Materials Pressure Vessel Design; 3) Carbon based Nanomaterials; 4) Nanotube production & purification; 5) Nanomechanics of Carbon Nanotubes; 6) CNT-composites: Example (Polymer); 7) Effect of Loading sequence on Composite with 8% by volume; 8) Models for Particulate Reinforced Composites; 9) Fullerene/Ti Composite for High Strength-Insulating Layer; 10) Fullerene/Epoxy Composite for High Strength-Insulating Layer; 11) Models for Continuous Fiber Reinforced Composites; 12) Tensile Strength for Discontinuous Fiber Composite; 13) Ti + SWNT Composites: Thermal/Mechanical; 14) Ti + SWNT Composites: Tensile Strength; and 15) Nano-structured Shell for Pressure Vessels.

Rocky Mountain evolution: Tying Continental Dynamics of the Rocky Mountains and Deep Probe seismic experiments with receiver functions

USGS Publications Warehouse

Rumpfhuber, E.-M.; Keller, Gordon R.; Sandvol, E.; Velasco, A.A.; Wilson, D.C.

2009-01-01

In this study, we have determined the crustal structure using three different receiver function methods using data collected from the northern transect of the Continental Dynamics of the Rocky Mountains (CD-ROM) experiment. The resulting migrated image and crustal thickness determinations confirm and refine prior crustal thickness measurements based on the CD-ROM and Deep Probe experiment data sets. The new results show a very distinct and thick lower crustal layer beneath the Archean Wyoming province. In addition, we are able to show its termination at 42??N latitude, which provides a seismic tie between the CD-ROM and Deep Probe seismic experiments and thus completes a continuous north-south transect extending from New Mexico into Alberta, Canada. This new tie is particularly important because it occurs close to a major tectonic boundary, the Cheyenne belt, between an Archean craton and a Proterozoic terrane. We used two different stacking techniques, based on a similar concept but using two different ways to estimate uncertainties. Furthermore, we used receiver function migration and common conversion point (CCP) stacking techniques. The combined interpretation of all our results shows (1) crustal thinning in southern Wyoming, (2) strong northward crustal thickening beginning in central Wyoming, (3) the presence of an unusually thick and high-velocity lower crust beneath the Wyoming province, and (4) the abrupt termination of this lower crustal layer north of the Cheyenne belt at 42??N latitude. Copyright 2009 by the American Geophysical Union.

49 CFR 572.74 - Thorax assembly and test procedure.

Code of Federal Regulations, 2010 CFR

2010-10-01

... by a test probe conforming to § 572.77(a) to 20 ±0.3 fps according to the test procedure in paragraph... longitudinal center line of the test probe so that it coincides with the designated impact point, and align the test probe so that at impact, the probe's longitudinal center line coincides (within 2 degrees) with...

49 CFR 572.74 - Thorax assembly and test procedure.

Code of Federal Regulations, 2012 CFR

2012-10-01

... by a test probe conforming to § 572.77(a) to 20 ±0.3 fps according to the test procedure in paragraph... longitudinal center line of the test probe so that it coincides with the designated impact point, and align the test probe so that at impact, the probe's longitudinal center line coincides (within 2 degrees) with...

49 CFR 572.74 - Thorax assembly and test procedure.

Code of Federal Regulations, 2014 CFR

2014-10-01

... by a test probe conforming to § 572.77(a) to 20 ±0.3 fps according to the test procedure in paragraph... longitudinal center line of the test probe so that it coincides with the designated impact point, and align the test probe so that at impact, the probe's longitudinal center line coincides (within 2 degrees) with...

49 CFR 572.74 - Thorax assembly and test procedure.

Code of Federal Regulations, 2013 CFR

2013-10-01

... by a test probe conforming to § 572.77(a) to 20 ±0.3 fps according to the test procedure in paragraph... longitudinal center line of the test probe so that it coincides with the designated impact point, and align the test probe so that at impact, the probe's longitudinal center line coincides (within 2 degrees) with...

49 CFR 572.74 - Thorax assembly and test procedure.

Code of Federal Regulations, 2011 CFR

2011-10-01

... by a test probe conforming to § 572.77(a) to 20 ±0.3 fps according to the test procedure in paragraph... longitudinal center line of the test probe so that it coincides with the designated impact point, and align the test probe so that at impact, the probe's longitudinal center line coincides (within 2 degrees) with...

Transferring Files Between the Deep Impact Spacecrafts and the Ground Data System Using the CCSDS File Delivery Protocol (CFDP): A Case Study

NASA Technical Reports Server (NTRS)

Sanders, Felicia A.; Jones, Grailing, Jr.; Levesque, Michael

2006-01-01

The CCSDS File Delivery Protocol (CFDP) Standard could reshape ground support architectures by enabling applications to communicate over the space link using reliable-symmetric transport services. JPL utilized the CFDP standard to support the Deep Impact Mission. The architecture was based on layering the CFDP applications on top of the CCSDS Space Link Extension Services for data transport from the mission control centers to the ground stations. On July 4, 2005 at 1:52 A.M. EDT, the Deep Impact impactor successfully collided with comet Tempel 1. During the final 48 hours prior to impact, over 300 files were uplinked to the spacecraft, while over 6 thousand files were downlinked from the spacecraft using the CFDP. This paper uses the Deep Impact Mission as a case study in a discussion of the CFDP architecture, Deep Impact Mission requirements, and design for integrating the CFDP into the JPL deep space support services. Issues and recommendations for future missions using CFDP are also provided.

Parallel Architectures for Planetary Exploration Requirements (PAPER)

NASA Technical Reports Server (NTRS)

Cezzar, Ruknet; Sen, Ranjan K.

1989-01-01

The Parallel Architectures for Planetary Exploration Requirements (PAPER) project is essentially research oriented towards technology insertion issues for NASA's unmanned planetary probes. It was initiated to complement and augment the long-term efforts for space exploration with particular reference to NASA/LaRC's (NASA Langley Research Center) research needs for planetary exploration missions of the mid and late 1990s. The requirements for space missions as given in the somewhat dated Advanced Information Processing Systems (AIPS) requirements document are contrasted with the new requirements from JPL/Caltech involving sensor data capture and scene analysis. It is shown that more stringent requirements have arisen as a result of technological advancements. Two possible architectures, the AIPS Proof of Concept (POC) configuration and the MAX Fault-tolerant dataflow multiprocessor, were evaluated. The main observation was that the AIPS design is biased towards fault tolerance and may not be an ideal architecture for planetary and deep space probes due to high cost and complexity. The MAX concepts appears to be a promising candidate, except that more detailed information is required. The feasibility for adding neural computation capability to this architecture needs to be studied. Key impact issues for architectural design of computing systems meant for planetary missions were also identified.

The Impact of Probe Variability on Brief Experimental Analysis of Reading Skills

ERIC Educational Resources Information Center

Mercer, Sterett H.; Harpole, Lauren Lestremau; Mitchell, Rachel R.; McLemore, Chandler; Hardy, Christina

2012-01-01

The purpose of this study was to examine the impact of probe variability on the ability to replicate results in brief experimental analysis (BEA) of reading. In the first phase of the study, 41 first- and second- grade students completed 16 oral reading fluency probes. Calculations of probe difficulty were used to identify Low and High Variability…

Big, Deep, and Smart Data in Scanning Probe Microscopy.

PubMed

Kalinin, Sergei V; Strelcov, Evgheni; Belianinov, Alex; Somnath, Suhas; Vasudevan, Rama K; Lingerfelt, Eric J; Archibald, Richard K; Chen, Chaomei; Proksch, Roger; Laanait, Nouamane; Jesse, Stephen

2016-09-27

Scanning probe microscopy (SPM) techniques have opened the door to nanoscience and nanotechnology by enabling imaging and manipulation of the structure and functionality of matter at nanometer and atomic scales. Here, we analyze the scientific discovery process in SPM by following the information flow from the tip-surface junction, to knowledge adoption by the wider scientific community. We further discuss the challenges and opportunities offered by merging SPM with advanced data mining, visual analytics, and knowledge discovery technologies.

Radiometric Spacecraft Tracking for Deep Space Navigation

NASA Technical Reports Server (NTRS)

Lanyi, Gabor E.; Border, James S.; Shin, Dong K.

2008-01-01

Interplanetary spacecraft navigation relies on three types of terrestrial tracking observables.1) Ranging measures the distance between the observing site and the probe. 2) The line-of-sight velocity of the probe is inferred from Doppler-shift by measuring the frequency shift of the received signal with respect to the unshifted frequency. 3) Differential angular coordinates of the probe with respect to natural radio sources are nominally obtained via a differential delay technique of (Delta) DOR (Delta Differential One-way Ranging). The accuracy of spacecraft coordinate determination depends on the measurement uncertainties associated with each of these three techniques. We evaluate the corresponding sources of error and present a detailed error budget.

Deep Impact Spots Quarry

NASA Technical Reports Server (NTRS)

2005-01-01

Sixty-nine days before it gets up-close-and-personal with a comet, NASA's Deep Impact spacecraft successfully photographed its quarry, comet Tempel 1, at a distance of 39.7 million miles. The image, taken on April 25, 2005, is the first of many comet portraits Deep Impact will take leading up to its historic comet encounter on July 4.

Subsurface Structure in the Martian Polar Layered Deposits: The Deep Space 2 Impact Accelerometry Experiment

NASA Technical Reports Server (NTRS)

Moersch, J. E.; Lorenz, R. D.

1998-01-01

While primarily a technology demonstration mission, the New Millenium Mars Microprobes (also known as Deep Space 2, or simply DS2)will also provide the first in situ science measurements of the martian subsurface. The DS2 impact accelerometry experiment will provide both engineering data about the depth of probe emplacement and science data about the physical nature of the subsurface at the probes' landing sites. Little is known about the detailed physical properties or small-scale vertical structure of the subsurface at the DS2 landing site in the southern martian polar layered deposits. Imaging data from the Viking Orbiters and Mars Global Surveyor reveal alternating bands of light and dark material in this region with thicknesses at least as small as the limit of resolution, about 10 m. The overall composition of these layers is poorly constrained, but generally thought to be a mixture of dust and ice with the layers being caused by variations in the dust/ice ratio, or perhaps by dust deposits of different densities. Low thermal inertias in the region suggest that the top few centimeters of the surface are composed of a mantling of fine-grained dust. However, 3.5-cm radar returns indicate that the maximum depth of this dust is not greater than a few tens of centimeters. Thermal models generally agree that, while the layered deposits do provide a potential near-surface reservoir for ice, the uppermost few centimeters to meters in these regions are likely to be ice-free because of sublimation losses. Finally, while it is generally agreed that the layered deposits are the product of variations in the martian climate, no direct correlation has been made between band sequences and specific climate changes. Our intention is to shed light on some of these questions about the martian polar layered deposits by using the DS2 accelerometry experiment to determine the physical nature of the layered deposits, and to detect the presence of any subsurface layering of dust, ice, and/or rock. In the process, we will also determine the final resting depth of the two microprobes, an important parameter in the interpretation of other DS2 experiments.

Safety and Effectiveness of a Longer Focal Beam and Burst Duration in Ultrasonic Propulsion for Repositioning Urinary Stones and Fragments.

PubMed

Janssen, Karmon M; Brand, Timothy C; Cunitz, Bryan W; Wang, Yak-Nam; Simon, Julianna C; Starr, Frank; Liggitt, H Denny; Thiel, Jeff; Sorensen, Mathew D; Harper, Jonathan D; Bailey, Michael R; Dunmire, Barbrina

2017-08-01

In the first-in-human trial of ultrasonic propulsion, subjects passed collections of residual stone fragments repositioned with a C5-2 probe. Here, effectiveness and safety in moving multiple fragments are compared between the C5-2 and a custom (SC-50) probe that produces a longer focal beam and burst duration. Effectiveness was quantified by the number of stones expelled from a calyx phantom consisting of a 30-mm deep, water-filled well in a block of tissue mimicking material. Each probe was positioned below the phantom to move stones against gravity. Single propulsion bursts of 50 ms or 3 s duration were applied to three separate targets: 10 fragments of 2 different sizes (1-2 and 2-3 mm) and a single 4 × 7 mm human stone. Safety studies consisted of porcine kidneys exposed to an extreme dose of 10-minute burst duration, including a 7-day survival study and acute studies with surgically implanted stones. Although successful in the clinical trial, the shorter focal beam and maximum 50 ms burst duration of the C5-2 probe moved stones, but did not expel any stones from the phantom's 30-mm deep calyx. The results were similar with the SC-50 probe under the same 50 ms burst duration. Longer (3 s) bursts available with the SC-50 probe expelled all stones at both 4.5 and 9.5 cm "skin-to-stone" depths with lower probe heating compared to the C5-2. No abnormal behavior, urine chemistry, serum chemistry, or histological findings were observed within the kidney or surrounding tissues for the 10 min burst duration used in the animal studies. A longer focal beam and burst duration improved expulsion of a stone and multiple stone fragments from a phantom over a broad range of clinically relevant penetration depths and did not cause kidney injury in animal studies.

KSC-05PD-0128

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. On Launch Pad 17-B, Cape Canaveral Air Force Station, Fla., the Boeing Delta II rocket carrying the Deep Impact spacecraft stands out against an early dawn sky. Scheduled for liftoff at 1:47 p.m. EST today, Deep Impact will head for space and a rendezvous with Comet Tempel 1 when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile (impactor) to crash onto the surface July 4, 2005, Deep Impacts flyby spacecraft will reveal the secrets of the comets interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-05PD-0124

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. On Launch Pad 17-B, Cape Canaveral Air Force Station, Fla., the Boeing Delta II rocket carrying the Deep Impact spacecraft is bathed in light waiting for tower rollback before launch. Scheduled for liftoff at 1:47 p.m. EST today, Deep Impact will head for space and a rendezvous with Comet Tempel 1 when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile (impactor) to crash onto the surface July 4, 2005, Deep Impacts flyby spacecraft will reveal the secrets of the comets interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

Radar Probing of Planetary Regoliths: An Example from the Northern Rim of Imbrium Basin

NASA Technical Reports Server (NTRS)

Thompson, Thomas W.; Campbell, Bruce A.; Ghent, Rebecca R.; Hawke, B. Ray; Leverington, David W.

2006-01-01

Imaging radar measurements at long wavelengths (e.g., >30 cm) allow deep (up to tens of meters) probing of the physical structure and dielectric properties of planetary regoliths. We illustrate a potential application for a Mars orbital synthetic aperture radar (SAR) using new Earth-based 70-cm wavelength radar data for the Moon. The terrae on the northern margin of Mare Imbrium, the Montes Jura region, have diffuse radar backscatter echoes that are 2-4 times weaker at 3.8-cm, 70-cm, and 7.5-m wavelengths than most other lunar nearside terrae. Possible geologic explanations are (1) a pyroclastic deposit associated with sinuous rilles in this region, (2) buried mare basalt or a zone of mixed highland/basaltic debris (cryptomaria), or (3) layers of ejecta associated with the Iridum and Plato impacts that have fewer meter-sized rocks than typical highlands regolith. While radar data at 3.8-cm to 7.5-m wavelengths suggest significant differences between the Montes Jura region and typical highlands, the surface geochemistry and rock abundance inferred from Clementine UV-VIS data and eclipse thermal images are consistent with other lunar terrae. There is no evidence for enhanced iron abundance, expected for basaltic pyroclastic deposits, near the source vents of the sinuous rilles radial to Plato. The regions of low 70-cm radar return are consistent with overlapping concentric ''haloes'' about Iridum and Plato and do not occur referentially in topographically low areas, as is observed for radar-mapped cryptomaria. Thus we suggest that the extensive radar-dark area associated with the Montes Jura region is due to overlapping, rock-poor ejecta deposits from Iridum and Plato craters. Comparison of the radial extent of low-radar-return crater haloes with a model for ejecta thickness shows that these rock-poor layers are detected by 70-cm radar where they are on the order of 10 m and thicker. A SAR in orbit about Mars could use similar deep probing to reveal the nature of crater - and basin-related deposits.

A new look at deep-sea video

USGS Publications Warehouse

Chezar, H.; Lee, J.

1985-01-01

A deep-towed photographic system with completely self-contained recording instrumentation and power can obtain color-video and still-photographic transects along rough terrane without need for a long electrically conducting cable. Both the video- and still-camera systems utilize relatively inexpensive and proven off-the-shelf hardware adapted for deep-water environments. The small instrument frame makes the towed sled an ideal photographic tool for use on ship or small-boat operations. The system includes a temperature probe and altimeter that relay data acoustically from the sled to the surface ship. This relay enables the operator to monitor simultaneously water temperature and the precise height off the bottom. ?? 1985.

Development of a new mini-invasive tumour hyperthermia probe using high-temperature water vapour.

PubMed

Yu, Tian-Hua; Zhou, Yi-Xin; Liu, Jing

2004-01-01

A new mini-invasive hyperthermia probe using high-temperature water vapour for deep regional tumour treatment was developed in this paper. The vacuum insulation mechanism was incorporated into the probe to avoid heating damage to the normal tissues around the edge of the insertion path. To better understand the heat transfer behaviour in living tissues due to operation of the probe, theoretical models based on the Pennes' equation were established and two closed form analytical solutions under constant flux or temperature heating at the tip of probe were obtained. Parametric studies were performed to investigate the influence of various parameters on the temperature response of tissues heated by the probe. Further, several simulating experiments on the actual heating performance of the probe fabricated in this paper were conducted on the in vitro biological materials (fresh pork) and phantom gel. It was demonstrated that the probe can cause a high enough temperature over the treatment area to thermally destroy the tumour tissue in due time, while the temperature over the surrounding healthy tissues can be kept below a safe threshold value. This mini-invasive heating probe may have significant applications in future clinical tumour hyperthermia.

Activatable Fluorescence Probe via Self-Immolative Intramolecular Cyclization for Histone Deacetylase Imaging in Live Cells and Tissues.

PubMed

Liu, Xianjun; Xiang, Meihao; Tong, Zongxuan; Luo, Fengyan; Chen, Wen; Liu, Feng; Wang, Fenglin; Yu, Ru-Qin; Jiang, Jian-Hui

2018-05-01

Histone deacetylases (HDACs) play essential roles in transcription regulation and are valuable theranostic targets. However, there are no activatable fluorescent probes for imaging of HDAC activity in live cells. Here, we develop for the first time a novel activatable two-photon fluorescence probe that enables in situ imaging of HDAC activity in living cells and tissues. The probe is designed by conjugating an acetyl-lysine mimic substrate to a masked aldehyde-containing fluorophore via a cyanoester linker. Upon deacetylation by HDAC, the probe undergoes a rapid self-immolative intramolecular cyclization reaction, producing a cyanohydrin intermediate that is spontaneously rapidly decomposed into the highly fluorescent aldehyde-containing two-photon fluorophore. The probe is shown to exhibit high sensitivity, high specificity, and fast response for HDAC detection in vitro. Imaging studies reveal that the probe is able to directly visualize and monitor HDAC activity in living cells. Moreover, the probe is demonstrated to have the capability of two-photon imaging of HDAC activity in deep tissue slices up to 130 μm. This activatable fluorescent probe affords a useful tool for evaluating HDAC activity and screening HDAC-targeting drugs in both live cell and tissue assays.

Deep wound cultures correlate well with bone biopsy culture in diabetic foot osteomyelitis.

PubMed

Malone, M; Bowling, F L; Gannass, A; Jude, E B; Boulton, A J M

2013-10-01

Osteomyelitis is a major complication in patients with diabetic foot ulceration. Accurate pathogenic identification of organisms can aid the clinician to a specific antibiotic therapy thereby preventing the need for amputation. All diabetic patients with bone biopsy-confirmed osteomyelitis were included into the study: biopsies were performed either during surgical removal of infected bone or percutaneously under guided fluoroscopy through non-infected tissue. The depth and extent of the ulcer was assessed using a sterile blunt metal probe. Deep wound cultures were taken from the wound base after sharp debridement. Of 66 cases of suspected osteomyelitis in 102 joints, 34 patients had both bone biopsies and deep wound cultures over the study period. Thirty two of 34 (94%), had a history of preceding foot ulceration, and in 25 of the cases a positive probe to bone test was recorded. In a high proportion of patients, at least one similar organism was isolated from both the deep wound culture and bone biopsy procedures (25 of 34 cases, 73.5%, p<0.001). When organisms were isolated from both wound cultures and bone biopsies, the identical strain was identified in both procedures in a significant proportion of cases (16 of 25 cases, 64%, p<0.001, total sample analysis in 16 of 34 cases, 47%). Deep wound cultures correlate well with osseous cultures and provide a sensitive method in assessing and targeting likely pathogens that cause osseous infections. This will help aid the clinician in guiding antibiotic therapy in centers where bone biopsies may not be readily available. Copyright © 2013 John Wiley & Sons, Ltd.

Using membrane composition to fine-tune the pKa of an optical liposome pH sensor.

PubMed

Clear, Kasey J; Virga, Katelyn; Gray, Lawrence; Smith, Bradley D

2016-04-14

Liposomes containing membrane-anchored pH-sensitive optical probes are valuable sensors for monitoring pH in various biomedical samples. The dynamic range of the sensor is maximized when the probe p K a is close to the expected sample pH. While some biomedical samples are close to neutral pH there are several circumstances where the pH is 1 or 2 units lower. Thus, there is a need to fine-tune the probe p K a in a predictable way. This investigation examined two lipid-conjugated optical probes, each with appended deep-red cyanine dyes containing indoline nitrogen atoms that are protonated in acid. The presence of anionic phospholipids in the liposomes stabilized the protonated probes and increased the probe p K a values by < 1 unit. The results show that rational modification of the membrane composition is a general non-covalent way to fine-tune the p K a of an optical liposome sensor for optimal pH sensing performance.

Characterisation of dry powder inhaler formulations using atomic force microscopy.

PubMed

Weiss, Cordula; McLoughlin, Peter; Cathcart, Helen

2015-10-15

Inhalation formulations are a popular way of treating the symptoms of respiratory diseases. The active pharmaceutical ingredient (API) is delivered directly to the site of action within the deep lung using an inhalation device such as the dry powder inhaler (DPI). The performance of the formulation and the efficiency of the treatment depend on a number of factors including the forces acting between the components. In DPI formulations these forces are dominated by interparticulate interactions. Research has shown that adhesive and cohesive forces depend on a number of particulate properties such as size, surface roughness, crystallinity, surface energetics and combinations of these. With traditional methods the impact of particulate properties on interparticulate forces could be evaluated by examining the bulk properties. Atomic force microscopy (AFM), however, enables the determination of local surface characteristics and the direct measurement of interparticulate forces using the colloidal probe technique. AFM is considered extremely useful for evaluating the surface topography of a substrate (an API or carrier particle) and even allows the identification of crystal faces, defects and polymorphs from high-resolution images. Additionally, information is given about local mechanical properties of the particles and changes in surface composition and energetics. The assessment of attractive forces between two bodies is possible by using colloidal probe AFM. This review article summarises the application of AFM in DPI formulations while specifically focussing on the colloidal probe technique and the evaluation of interparticulate forces. Copyright © 2015 Elsevier B.V. All rights reserved.

Deep Atmosphere Ammonia Mixing Ratio at Jupiter from the Galileo Probe Mass Spectrometer

NASA Technical Reports Server (NTRS)

Mahaffy, P. R.; Niemann, H. B.; Demick, J. E.

1999-01-01

New laboratory studies employing the Engineering Unit (EU) of the Galileo Probe Mass Spectrometer (GPMS) have resulted in a substantial reduction in the previously reported upper limit on the ammonia mixing ratio derived from the GPMS experiment at Jupiter. This measurement is complicated by background ammonia contributions in the GPMS during direct atmospheric sampling produced from the preceding gas enrichment experiments. These backgrounds can be quantified with the data from the EU studies when they are carried out in a manner that duplicates the descent profile of pressure and enrichment cell loading. This background is due to the tendency of ammonia to interact strongly with the walls of the mass spectrometer and on release to contribute to the gas being directly directed into the ion source from the atmosphere through a capillary pressure reduction leak. It is evident from the GPMS and other observations that the mixing ratio of ammonia at Jupiter reaches the deep atmosphere value at substantially higher pressures than previously assumed. This is a likely explanation for the previously perceived discrepancy between ammonia values derived from ground based microwave observations and those obtained from attenuation of the Galileo Probe radio signal.

Restoration of the Apollo Heat Flow Experiments Metadata

NASA Technical Reports Server (NTRS)

Nagihara, S.; Stephens, M. K.; Taylor, P. T.; Williams, D. R.; Hills, H. K.; Nakamura, Y.

2015-01-01

Geothermal heat flow probes were deployed on the Apollo 15 and 17 missions as part of the Apollo Lunar Surface Experiments Package (ALSEP). At each landing site, the astronauts drilled 2 holes, 10-m apart, and installed a probe in each. The holes were 1- and 1.5-m deep at the Apollo 15 site and 2.5-m deep at the Apollo 17 sites. The probes monitored surface temperature and subsurface temperatures at different depths. At the Apollo 15 site, the monitoring continued from July 1971 to January 1977. At the Apollo 17 site, it did from December 1972 to September 1977. Based on the observations made through December 1974, Marcus Langseth, the principal investigator of the heat flow experiments (HFE), determined the thermal conductivity of the lunar regolith by mathematically modeling how the seasonal temperature fluctuation propagated down through the regolith. He also determined the temperature unaffected by diurnal and seasonal thermal waves of the regolith at different depths, which yielded the geothermal gradient. By multiplying the thermal gradient and the thermal conductivity, Langseth obtained the endogenic heat flow of the Moon as 21 mW/m(exp 2) at Site 15 and 16 mW/m(exp 2) at Site 17.

A correlation between the defect states and yellow luminescence in AlGaN/GaN heterostructures

NASA Astrophysics Data System (ADS)

Jana, Dipankar; Sharma, T. K.

2017-07-01

AlGaN/GaN heterostructures are investigated by performing complementary spectroscopic measurements under novel experimental configurations. Distinct features related to the band edge of AlGaN and GaN layers are clearly observed in surface photovoltage spectroscopy (SPS) spectra. A few more SPS features, which are associated with defects in GaN, are also identified by performing the pump-probe SPS measurements. SPS results are strongly corroborated by the complementary photoluminescence and photoluminescence excitation (PLE) measurements. A correlation between the defect assisted SPS features and yellow luminescence (YL) peak is established by performing pump-probe SPS and PLE measurements. It is found that CN-ON donor complex is responsible for the generation of YL peak in our sample. Further, the deep trap states are found to be present throughout the entire GaN epilayer. It is also noticed that the deep trap states lying at the GaN/Fe-GaN interface make a strong contribution to the YL feature. A phenomenological model is proposed to explain the intensity dependence of the YL feature and the corresponding SPS features in a pump-probe configuration, where a reasonable agreement between the numerical simulations and experimental results is achieved.

Magnetoresistive flux focusing eddy current flaw detection

NASA Technical Reports Server (NTRS)

Wincheski, Russell A. (Inventor); Simpson, John W. (Inventor); Namkung, Min (Inventor)

2005-01-01

A giant magnetoresistive flux focusing eddy current device effectively detects deep flaws in thick multilayer conductive materials. The probe uses an excitation coil to induce eddy currents in conducting material perpendicularly oriented to the coil's longitudinal axis. A giant magnetoresistive (GMR) sensor, surrounded by the excitation coil, is used to detect generated fields. Between the excitation coil and GMR sensor is a highly permeable flux focusing lens which magnetically separates the GMR sensor and excitation coil and produces high flux density at the outer edge of the GMR sensor. The use of feedback inside the flux focusing lens enables complete cancellation of the leakage fields at the GMR sensor location and biasing of the GMR sensor to a location of high magnetic field sensitivity. In an alternate embodiment, a permanent magnet is positioned adjacent to the GMR sensor to accomplish the biasing. Experimental results have demonstrated identification of flaws up to 1 cm deep in aluminum alloy structures. To detect deep flaws about circular fasteners or inhomogeneities in thick multilayer conductive materials, the device is mounted in a hand-held rotating probe assembly that is connected to a computer for system control, data acquisition, processing and storage.

Fiber-based tissue identification for electrode placement in deep brain stimulation neurosurgery (Conference Presentation)

NASA Astrophysics Data System (ADS)

DePaoli, Damon T.; Lapointe, Nicolas; Goetz, Laurent; Parent, Martin; Prudhomme, Michel; Cantin, Léo.; Galstian, Tigran; Messaddeq, Younès.; Côté, Daniel C.

2016-03-01

Deep brain stimulation's effectiveness relies on the ability of the stimulating electrode to be properly placed within a specific target area of the brain. Optical guidance techniques that can increase the accuracy of the procedure, without causing any additional harm, are therefore of great interest. We have designed a cheap optical fiber-based device that is small enough to be placed within commercially available DBS stimulating electrodes' hollow cores and that is capable of sensing biological information from the surrounding tissue, using low power white light. With this probe we have shown the ability to distinguish white and grey matter as well as blood vessels, in vitro, in human brain samples and in vivo, in rats. We have also repeated the in vitro procedure with the probe inserted in a DBS stimulating electrode and found the results were in good agreement. We are currently validating a second fiber optic device, with micro-optical components, that will result in label free, molecular level sensing capabilities, using CARS spectroscopy. The final objective will be to use this data in real time, during deep brain stimulation neurosurgery, to increase the safety and accuracy of the procedure.

Magnetoresistive Flux Focusing Eddy Current Flaw Detection

NASA Technical Reports Server (NTRS)

Wincheski, Russell A. (Inventor); Namkung, Min (Inventor); Simpson, John W. (Inventor)

2005-01-01

A giant magnetoresistive flux focusing eddy current device effectively detects deep flaws in thick multilayer conductive materials. The probe uses an excitation coil to induce eddy currents in conducting material perpendicularly oriented to the coil s longitudinal axis. A giant magnetoresistive (GMR) sensor, surrounded by the excitation coil, is used to detect generated fields. Between the excitation coil and GMR sensor is a highly permeable flux focusing lens which magnetically separates the GMR sensor and excitation coil and produces high flux density at the outer edge of the GMR sensor. The use of feedback inside the flux focusing lens enables complete cancellation of the leakage fields at the GMR sensor location and biasing of the GMR sensor to a location of high magnetic field sensitivity. In an alternate embodiment, a permanent magnet is positioned adjacent to the GMR sensor to accomplish the biasing. Experimental results have demonstrated identification of flaws up to 1 cm deep in aluminum alloy structures. To detect deep flaws about circular fasteners or inhomogeneities in thick multi-layer conductive materials, the device is mounted in a hand-held rotating probe assembly that is connected to a computer for system control, data acquisition, processing and storage.

Physical limitations in sensors for a drag-free deep space probe

NASA Technical Reports Server (NTRS)

Juillerat, R.

1971-01-01

The inner perturbing forces acting on sensors were analyzed, taking into account the technological limitations imposed on the proof mass position pickup and proof mass acquisition system. The resulting perturbing accelerations are evaluated as a function of the drag-free sensor parameters. Perturbations included gravitational attraction, electrical action, magnetic action, pressure effects, radiation effects, and action of the position pickup. These data can be used to study the laws of guidance, providing an optimization of the space probe as a whole.

Optical Probing of Low-Pressure Solution Grown GaN Crystal Properties

DTIC Science & Technology

2010-04-01

observed in Mg and Si doped epitaxial films deposited by MBE and MOCVD on freestanding GaN HVPE substrates [23–25]. Considering the purity of the precursors...bands with similar energy positions here reported, a dominant deeper acceptor impurity has been assigned to Zn , a well known deep acceptor in GaN . Room...00-00-2010 to 00-00-2010 4. TITLE AND SUBTITLE Optical probing of low-pressure solution grown GaN crystal properties 5a. CONTRACT NUMBER 5b

TEMPEST: Twin Electric Magnetospheric Probes Exploring on Spiral Trajectories--A Proposal to the Medium Class Explorer Program

NASA Technical Reports Server (NTRS)

1995-01-01

The objective of the Twin Electric Magnetospheric Probes Exploring on Spiral Trajectories (TEMPEST) mission is to understand the nature and causes of magnetic storm conditions in the magnetosphere whether they be manifested classically in the buildup of the ring current, or (as recently discovered) by storms of relativistic electrons that cause the deep dielectric charging responsible for disabling satellites in synchronous orbit, or by the release of energy into the auroral ionosphere and the plasma sheet during substorms.

Deep ultraviolet resonant Raman imaging of a cell

NASA Astrophysics Data System (ADS)

Kumamoto, Yasuaki; Taguchi, Atsushi; Smith, Nicholas Isaac; Kawata, Satoshi

2012-07-01

We report the first demonstration of deep ultraviolet (DUV) Raman imaging of a cell. Nucleotide distributions in a HeLa cell were observed without any labeling at 257 nm excitation with resonant bands attributable to guanine and adenine. Obtained images represent DNA localization at nucleoli in the nucleus and RNA distribution in the cytoplasm. The presented technique extends the potential of Raman microscopy as a tool to selectively probe nucleic acids in a cell with high sensitivity due to resonance.

JPL-20180505-INSIGHf-0001-NASA InSight on Its Way to Mars

NASA Image and Video Library

2018-05-05

Making history as the first interplanetary launch from the West Coast, NASA's InSight spacecraft is now soaring towards Mars. The spacecraft, which lifted off from Vandenberg Air Force Base in Central California, will be the first mission to study the deep interior of Mars. Its instruments include a seismometer to detect marsquakes for the first time and a heat flow p[robe that will embed itself as deep as about 16 feet (5 meters) below the surface of Mars.

KSC-05PD-0140

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. NASA Administrator Sean OKeefe reports to employees on the state of the Agency from the Press Site Auditorium. The update was broadcast live via NASA Television. O'Keefe focused on the achievements of 2004 and the goals set for 2005. His remarks emphasized the milestones met in NASA's Vision for Space Exploration, including the launch of the comet-chasing Deep Impact mission and the landing of the Huygens probe on Jupiters moon Titan, both occurring in the past two days, and the progress made in meeting the requirements to return the Space Shuttle to flight. OKeefes briefing included a dialogue with Associate Administrator of NASAs Office of Exploration Systems Craig Steidle and Center Director Jim Kennedy, live; and Manager of the Space Station Office Bill Gerstenmaier and Director of Advanced Planning and Jet Propulsion Laboratory Charles Elachi, via satellite.

KSC-05PD-0141

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. NASA Administrator Sean OKeefe reports to employees on the state of the Agency from the Press Site Auditorium. The update was broadcast live via NASA Television. O'Keefe focused on the achievements of 2004 and the goals set for 2005. His remarks emphasized the milestones met in NASA's Vision for Space Exploration, including the launch of the comet-chasing Deep Impact mission and the landing of the Huygens probe on Jupiters moon Titan, both occurring in the past two days, and the progress made in meeting the requirements to return the Space Shuttle to flight. OKeefes briefing included a dialogue with Associate Administrator of NASAs Office of Exploration Systems Craig Steidle and Center Director Jim Kennedy, live; and Manager of the Space Station Office Bill Gerstenmaier and Director of Advanced Planning and Jet Propulsion Laboratory Charles Elachi, via satellite.

Gravitational Lens: Deep Space Probe Design

DTIC Science & Technology

2012-03-01

Lieutenant, USAF Approved: Timothy Lawrence, Col, USAF (Chairman) Date Carl Hartsfield, Lt Col, USAF (Member) Date Marc G. Millis (Member) Date Abstract A...23 RTG Radioisotope Thermoelectric Generators . . . . . . . . . . . . . . . . . 26 EOL End of Life...26 ASRG Advanced Stirling Radioisotope Generator . . . . . . . . . . . . . . . . 26 GPHS

Gravity at the horizon: on relativistic effects, CMB-LSS correlations and ultra-large scales in Horndeski's theory

DOE Office of Scientific and Technical Information (OSTI.GOV)

Renk, Janina; Zumalacárregui, Miguel; Montanari, Francesco, E-mail: renk@thphys.uni-heidelberg.de, E-mail: miguel.zumalacarregui@nordita.org, E-mail: francesco.montanari@helsinki.fi

2016-07-01

We address the impact of consistent modifications of gravity on the largest observable scales, focusing on relativistic effects in galaxy number counts and the cross-correlation between the matter large scale structure (LSS) distribution and the cosmic microwave background (CMB). Our analysis applies to a very broad class of general scalar-tensor theories encoded in the Horndeski Lagrangian and is fully consistent on linear scales, retaining the full dynamics of the scalar field and not assuming quasi-static evolution. As particular examples we consider self-accelerating Covariant Galileons, Brans-Dicke theory and parameterizations based on the effective field theory of dark energy, using the himore » class code to address the impact of these models on relativistic corrections to LSS observables. We find that especially effects which involve integrals along the line of sight (lensing convergence, time delay and the integrated Sachs-Wolfe effect—ISW) can be considerably modified, and even lead to O(1000%) deviations from General Relativity in the case of the ISW effect for Galileon models, for which standard probes such as the growth function only vary by O(10%). These effects become dominant when correlating galaxy number counts at different redshifts and can lead to ∼ 50% deviations in the total signal that might be observable by future LSS surveys. Because of their integrated nature, these deep-redshift cross-correlations are sensitive to modifications of gravity even when probing eras much before dark energy domination. We further isolate the ISW effect using the cross-correlation between LSS and CMB temperature anisotropies and use current data to further constrain Horndeski models. Forthcoming large-volume galaxy surveys using multiple-tracers will search for all these effects, opening a new window to probe gravity and cosmic acceleration at the largest scales available in our universe.« less

Fast gain recovery rates with strong wavelength dependence in a non-linear SOA.

PubMed

Cleary, Ciaran S; Power, Mark J; Schneider, Simon; Webb, Roderick P; Manning, Robert J

2010-12-06

We report remarkably fast and strongly wavelength-dependent gain recovery in a single SOA without the aid of an offset filter. Full gain recovery times as short as 9 ps were observed in pump-probe measurements when pumping to the blue wavelength side of a continuous wave probe, in contrast to times of 25 to 30 ps when pumping to the red wavelength side. Experimental and numerical analysis indicate that the long effective length and high gain led to deep saturation of the second half of the SOA by the probe. The consequent absorption of blue-shifted pump pulses in this region resulted in device dynamics analogous to those of the Turbo-Switch.

Phantom testing of a novel endoscopic OCT probe: a prelude to clinical in-vivo laryngeal use

NASA Astrophysics Data System (ADS)

Tatla, Taran; Pang, J. Y.; Cernat, R.; Dobre, G.; Tadrous, P. J.; Bradu, A.; Gelikonov, G.; Gelikonov, V.; Podoleanu, A. G.

2012-12-01

Optical coherence tomography is a novel imaging technique providing potentially high resolution tri-dimensional images of tissue microstructure up to 2-3mm deep. We present pre-clinical data from a novel miniaturised OCT probe utilised for endoscopic imaging of laryngeal mucosa. A 1300nm SS-OCT probe was passed in tandem with a flexible fibreoptic nasoendoscope into the larynx of a manikin. Ex vivo OCT images were acquired using a desktop 1300nm TD-OCT imaging system. The feasibility, robustness and safety of this set-up was demonstrated as a preliminary step to extending the use of this assembly to a clinical patient cohort with varying laryngeal pathologies.

49 CFR 572.76 - Limbs assembly and test procedure.

Code of Federal Regulations, 2013 CFR

2013-10-01

.... (3) Align the test probe specified in § 572.77(a) with the longitudinal center line of the femur force gauge, so that at impact, the probe's longitudinal center line coincides with the sensor's longitudinal center line within ±2 degrees. (4) Impact the knee with the test probe moving horizontally and...

49 CFR 572.42 - Thorax.

Code of Federal Regulations, 2011 CFR

2011-10-01

... impact, is impacted by a test probe conforming to § 572.44(a) at 14 fps in accordance with paragraph (b... of the test probe at the lateral side of the chest at the intersection of the centerlines of the... side of the vehicle. The probe's centerline is perpendicular to thorax's midsagittal plane. (3) Align...

49 CFR 572.42 - Thorax.

Code of Federal Regulations, 2013 CFR

2013-10-01

... impact, is impacted by a test probe conforming to § 572.44(a) at 14 fps in accordance with paragraph (b... of the test probe at the lateral side of the chest at the intersection of the centerlines of the... side of the vehicle. The probe's centerline is perpendicular to thorax's midsagittal plane. (3) Align...

49 CFR 572.76 - Limbs assembly and test procedure.

Code of Federal Regulations, 2011 CFR

2011-10-01

.... (3) Align the test probe specified in § 572.77(a) with the longitudinal center line of the femur force gauge, so that at impact, the probe's longitudinal center line coincides with the sensor's longitudinal center line within ±2 degrees. (4) Impact the knee with the test probe moving horizontally and...

49 CFR 572.42 - Thorax.

Code of Federal Regulations, 2014 CFR

2014-10-01

... impact, is impacted by a test probe conforming to § 572.44(a) at 14 fps in accordance with paragraph (b... of the test probe at the lateral side of the chest at the intersection of the centerlines of the... side of the vehicle. The probe's centerline is perpendicular to thorax's midsagittal plane. (3) Align...

49 CFR 572.76 - Limbs assembly and test procedure.

Code of Federal Regulations, 2012 CFR

2012-10-01

.... (3) Align the test probe specified in § 572.77(a) with the longitudinal center line of the femur force gauge, so that at impact, the probe's longitudinal center line coincides with the sensor's longitudinal center line within ±2 degrees. (4) Impact the knee with the test probe moving horizontally and...

49 CFR 572.42 - Thorax.

Code of Federal Regulations, 2012 CFR

2012-10-01

... impact, is impacted by a test probe conforming to § 572.44(a) at 14 fps in accordance with paragraph (b... of the test probe at the lateral side of the chest at the intersection of the centerlines of the... side of the vehicle. The probe's centerline is perpendicular to thorax's midsagittal plane. (3) Align...

49 CFR 572.76 - Limbs assembly and test procedure.

Code of Federal Regulations, 2014 CFR

2014-10-01

.... (3) Align the test probe specified in § 572.77(a) with the longitudinal center line of the femur force gauge, so that at impact, the probe's longitudinal center line coincides with the sensor's longitudinal center line within ±2 degrees. (4) Impact the knee with the test probe moving horizontally and...

KSC-05PD-0126

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. On Launch Pad 17-B, Cape Canaveral Air Force Station, Fla., shadows paint the Boeing Delta II rocket carrying the Deep Impact spacecraft as the mobile service tower at left is rolled back before launch.Scheduled for liftoff at 1:47 p.m. EST today, Deep Impact will head for space and a rendezvous with Comet Tempel 1 when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile (impactor) to crash onto the surface July 4, 2005, Deep Impacts flyby spacecraft will reveal the secrets of the comets interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-05PD-0125

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. On Launch Pad 17-B, Cape Canaveral Air Force Station, Fla., the Boeing Delta II rocket carrying the Deep Impact spacecraft looms into the night sky as the mobile service tower at right is rolled back before launch. Scheduled for liftoff at 1:47 p.m. EST today, Deep Impact will head for space and a rendezvous with Comet Tempel 1 when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile (impactor) to crash onto the surface July 4, 2005, Deep Impacts flyby spacecraft will reveal the secrets of the comets interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-05PD-0127

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. On Launch Pad 17-B, Cape Canaveral Air Force Station, Fla., the Boeing Delta II carrying the Deep Impact spacecraft rocket shines under spotlights in the early dawn hours as it waits for launch. Scheduled for liftoff at 1:47 p.m. EST today, Deep Impact will head for space and a rendezvous with Comet Tempel 1 when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile (impactor) to crash onto the surface July 4, 2005, Deep Impacts flyby spacecraft will reveal the secrets of the comets interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

KSC-05PD-0129

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. The sun rises behind Launch Pad 17-B, Cape Canaveral Air Force Station, Fla., where the Boeing Delta II rocket carrying the Deep Impact spacecraft waits for launch. Gray clouds above the horizon belie the favorable weather forecast for the afternoon launch. Scheduled for liftoff at 1:47 p.m. EST today, Deep Impact will head for space and a rendezvous with Comet Tempel 1 when the comet is 83 million miles from Earth. After releasing a 3- by 3-foot projectile (impactor) to crash onto the surface July 4, 2005, Deep Impacts flyby spacecraft will reveal the secrets of the comets interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network. Deep Impact is a NASA Discovery mission.

CEMERLL: The Propagation of an Atmosphere-Compensated Laser Beam to the Apollo 15 Lunar Array

NASA Technical Reports Server (NTRS)

Fugate, R. Q.; Leatherman, P. R.; Wilson, K. E.

1997-01-01

Adaptive optics techniques can be used to realize a robust low bit-error-rate link by mitigating the atmosphere-induced signal fades in optical communications links between ground-based transmitters and deep-space probes.

Solvatochromic probe behavior within choline chloride-based deep eutectic solvents: effect of temperature and water.

PubMed

Pandey, Ashish; Pandey, Siddharth

2014-12-18

Deep eutectic solvents (DESs) have shown potential as promising environmentally friendly alternatives to conventional solvents. Many common and popular DESs are obtained by simply mixing a salt and a H-bond donor. Properties of such a DES depend on its constituents. Change in temperature and addition of water, a benign cosolvent, can change the physicochemical properties of DESs. The effect of changing temperature and addition of water on solvatochromic probe behavior within three DESs formed from choline chloride combined with 1,2-ethanediol, glycerol, and urea, respectively, in 1:2 mol ratios termed ethaline, glyceline, and reline is presented. Increase in temperature results in reduced H-bond donating acidity of the DESs. Dipolarity/polarizability and H-bond accepting basicity do not change with changing temperature of the DESs. The response of the fluorescence probe pyrene also indicates a decrease in the polarity of the DESs as temperature is increased. Addition of water to DES results in increased dipolarity/polarizability and a decrease in H-bond accepting basicity. Except for pyrene, solvatochromic probes exhibit responses close to those predicted from ideal-additive behavior with slight preferential solvation by DES within the aqueous mixtures. Pyrene response reveals significant preferential solvation by DES and/or the presence of solvent-solvent interactions, especially within aqueous mixtures of ethaline and glyceline, the DESs constituted of H-bond donors with hydroxyl functionalities. FTIR absorbance and Raman spectroscopic measurements of aqueous DES mixtures support the outcomes from solvatochromic probe responses. Aqueous mixtures of ethaline and glyceline possess relatively more interspecies H-bonds as compared to aqueous mixtures of reline, where interstitial accommodation of water within the reline molecular network appears to dominate.

The Ejecta Evolution of Deep Impact: Insight from Experiments

NASA Astrophysics Data System (ADS)

Hermalyn, B.; Schultz, P. H.; Heineck, J. T.

2010-12-01

The Deep Impact (DI) probe impacted comet 9P/Tempel 1 at an angle of ~30° from local horizontal with a velocity of 10.2 km/s. Examination of the resulting ballistic (e.g., non-vapor driven) ejecta revealed phenomena that largely followed expectations from laboratory investigations of oblique impacts into low-density porous material, including a downrange bias, uprange zone of avoidance, and cardioid (curved) rays (Schultz, et al, 2005, 2007). Modeling of the impact based on canonical models and scaling laws (Richardson, et al, 2007) allowed a first-order reconstruction of the event, but did not fully represent the three-dimensional nature of the ejecta flow-field in an oblique impact essential for interpretation of the DI data. In this study, we present new experimental measurements of the early-time ejecta dynamics in oblique impacts that allow a more complete reconstruction of the ballistic ejecta from the impact, including visualization of the DI encounter and predictions for the upcoming re-encounter with Tempel 1. A suite of hypervelocity 30° impact experiments into granular materials was performed at the NASA Ames Vertical Gun Range (AVGR) for the purpose of interpreting the Deep Impact event. A technique based on Particle Tracking Velocimetry (PTV) permitted non-intrusive measurement of the ejecta velocity within the ejecta curtain. The PTV system developed at the AVGR utilizes a laser light sheet projected parallel to the impact surface to illuminate horizontal “slices” of the ejecta curtain that are then recorded by multiple cameras. Particle displacement between successive frames and cameras allows determination of the three-component velocity of the ejecta curtain. Pioneering efforts with a similar technique (Anderson, et al, 2003, 2006) characterized the main-stage ejecta velocity distributions and demonstrated that asymmetries in velocity and ejection angle persist well into the far-field for oblique impacts. In this study, high-speed cameras capture the time-resolved ejecta flow field in a regime much earlier than prior investigations, which permits reconstruction of the event in a temporal (rather than spatial) fashion. Preliminary results for 30° impacts into sand (Hermalyn, et al, 2010) show asymmetric, time-varying ejection angles throughout measurement of crater growth. The downrange component of ejecta initially has the lowest ejection angle. Incomplete coupling at early times forms the zone of avoidance uprange; once the curtain fully closes, this component exhibits a higher angle of ejection than the lateral or downrange regions. The convolution of the decreasing ejection velocities and coupling time leads to the appearance of “curved” rays in the uprange direction (Schultz, et al, 2009). All azimuths approach the same velocity trend towards the end of crater growth, as seen by Anderson, et al (2003). Reconstruction of the DI event by placing the scaled ejecta distribution from the experiments on the shape model (Thomas, et al, 2007) and matching the trajectory and view of the DI spacecraft permit comparison to the DI event. Investigation of the effect of target porosity and layering on the ejecta dynamics constrains the true nature of the impact conditions and surface structure of Tempel 1.

A closer look into deep eutectic solvents: exploring intermolecular interactions using solvatochromic probes.

PubMed

Florindo, C; McIntosh, A J S; Welton, T; Branco, L C; Marrucho, I M

2017-12-20

Deep eutectic solvents (DESs) constitute a new class of ionic solvents that has been developing at a fast pace in recent years. Since these solvents are commonly suggested as green alternatives to organic solvents, it is important to understand their physical properties. In particular, polarity plays an important role in solvation phenomena. In this work, the polarity of different families of DESs was studied through solvatochromic responses of UV-vis absorption probes. Kamlet-Taft α, β, π* and E T N parameters were evaluated using different solvatochromic probes, as 2,6-dichloro-4-(2,4,6-triphenyl-N-pyridino)-phenolate (Reichardt's betaine dye 33), 4-nitroaniline, and N,N-diethyl-4-nitroaniline for several families of DESs based on cholinium chloride, dl-menthol and a quaternary ammonium salt ([N 4444 ]Cl). In addition, a study to understand the difference in polarity properties between DESs and the corresponding ILs, namely ILs based on cholinium cation and carboxylic acids as anions ([Ch][Lev], [Ch][Gly] and [Ch][Mal]), was carried out. The chemical structure of the hydrogen bond acceptor (HBA) in a DES clearly controls the dipolarity/polarizability afforded by the DES. Moreover, Kamlet-Taft parameters do not vary much within the family, but they differ among families based on different HBA, either for DESs containing salts ([Ch]Cl or [N 4444 ]Cl) or neutral compounds (dl-menthol). A substitution of the HBD was also found to play an important role in solvatochromic probe behaviour for all the studied systems.

Development of Eddy Current Techniques for Detection of Deep Fatigue Cracks in Multi-Layer Airframe Components

NASA Technical Reports Server (NTRS)

Wincheski, Russell A.

2008-01-01

Thick, multi-layer aluminum structure has been widely used in aircraft design in critical wing splice areas. The multi-layer structure generally consists of three or four aluminum layers with different geometry and varying thickness, which are held together with fasteners. The detection of cracks under fasteners with ultrasonic techniques in subsurface layers away from the skin is impeded primarily by interlayer bonds and faying sealant condition. Further, assessment of such sealant condition is extremely challenging in terms of complexity of structure, limited access, and inspection cost. Although Eddy current techniques can be applied on in-service aircraft from the exterior of the skin without knowing sealant condition, the current eddy current techniques are not able to detect defects with wanted sensitivity. In this work a series of low frequency eddy current probes have been designed, fabricated and tested for this application. A probe design incorporating a shielded magnetic field sensor concentrically located in the interior of a drive coil has been employed to enable a localized deep diffusion of the electromagnetic field into the part under test. Due to the required low frequency inspections, probes have been testing using a variety of magnetic field sensors (pickup coil, giant magneto-resistive, anisotropic magneto-resistive, and spin-dependent tunneling). The probe designs as well as capabilities based upon a target inspection for sub-layer cracking in an airframe wing spar joint is presented.

An activity-based near-infrared glucuronide trapping probe for imaging β-glucuronidase expression in deep tissues.

PubMed

Cheng, Ta-Chun; Roffler, Steve R; Tzou, Shey-Cherng; Chuang, Kuo-Hsiang; Su, Yu-Cheng; Chuang, Chih-Hung; Kao, Chien-Han; Chen, Chien-Shu; Harn, I-Hong; Liu, Kuan-Yi; Cheng, Tian-Lu; Leu, Yu-Ling

2012-02-15

β-glucuronidase is an attractive reporter and prodrug-converting enzyme. The development of near-IR (NIR) probes for imaging of β-glucuronidase activity would be ideal to allow estimation of reporter expression and for personalized glucuronide prodrug cancer therapy in preclinical studies. However, NIR glucuronide probes are not yet available. In this work, we developed two fluorescent probes for detection of β-glucuronidase activity, one for the NIR range (containing IR-820 dye) and the other for the visible range [containing fluorescein isothiocyanate (FITC)], by utilizing a difluoromethylphenol-glucuronide moiety (TrapG) to trap the fluorochromes in the vicinity of the active enzyme. β-glucuronidase-mediated hydrolysis of the glucuronyl bond of TrapG generates a highly reactive alkylating group that facilitates the attachment of the fluorochrome to nucleophilic moieties located near β-glucuronidase-expressing sites. FITC-TrapG was selectively trapped on purified β-glucuronidase or β-glucuronidase-expressing CT26 cells (CT26/mβG) but not on bovine serum albumin or non-β-glucuronidase-expressing CT26 cells used as controls. β-glucuronidase-activated FITC-TrapG did not interfere with β-glucuronidase activity and could label bystander proteins near β-glucuronidase. Both FITC-TrapG and NIR-TrapG specifically imaged subcutaneous CT26/mβG tumors, but only NIR-TrapG could image CT26/mβG tumors transplanted deep in the liver. Thus NIR-TrapG may provide a valuable tool for visualizing β-glucuronidase activity in vivo.

Deep Learning of Atomically Resolved Scanning Transmission Electron Microscopy Images: Chemical Identification and Tracking Local Transformations

DOE PAGES

Ziatdinov, Maxim; Dyck, Ondrej; Maksov, Artem; ...

2017-12-07

Recent advances in scanning transmission electron and scanning probe microscopies have opened unprecedented opportunities in probing the materials structural parameters and various functional properties in real space with an angstrom-level precision. This progress has been accompanied by exponential increase in the size and quality of datasets produced by microscopic and spectroscopic experimental techniques. These developments necessitate adequate methods for extracting relevant physical and chemical information from the large datasets, for which a priori information on the structures of various atomic configurations and lattice defects is limited or absent. Here we demonstrate an application of deep neural networks to extracting informationmore » from atomically resolved images including location of the atomic species and type of defects. We develop a “weakly-supervised” approach that uses information on the coordinates of all atomic species in the image, extracted via a deep neural network, to identify a rich variety of defects that are not part of an initial training set. We further apply our approach to interpret complex atomic and defect transformation, including switching between different coordination of silicon dopants in graphene as a function of time, formation of peculiar silicon dimer with mixed 3-fold and 4-fold coordination, and the motion of molecular “rotor”. In conclusion, this deep learning based approach resembles logic of a human operator, but can be scaled leading to significant shift in the way of extracting and analyzing information from raw experimental data.« less

Deep Learning of Atomically Resolved Scanning Transmission Electron Microscopy Images: Chemical Identification and Tracking Local Transformations

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ziatdinov, Maxim; Dyck, Ondrej; Maksov, Artem

Recent advances in scanning transmission electron and scanning probe microscopies have opened unprecedented opportunities in probing the materials structural parameters and various functional properties in real space with an angstrom-level precision. This progress has been accompanied by exponential increase in the size and quality of datasets produced by microscopic and spectroscopic experimental techniques. These developments necessitate adequate methods for extracting relevant physical and chemical information from the large datasets, for which a priori information on the structures of various atomic configurations and lattice defects is limited or absent. Here we demonstrate an application of deep neural networks to extracting informationmore » from atomically resolved images including location of the atomic species and type of defects. We develop a “weakly-supervised” approach that uses information on the coordinates of all atomic species in the image, extracted via a deep neural network, to identify a rich variety of defects that are not part of an initial training set. We further apply our approach to interpret complex atomic and defect transformation, including switching between different coordination of silicon dopants in graphene as a function of time, formation of peculiar silicon dimer with mixed 3-fold and 4-fold coordination, and the motion of molecular “rotor”. In conclusion, this deep learning based approach resembles logic of a human operator, but can be scaled leading to significant shift in the way of extracting and analyzing information from raw experimental data.« less

Deep Learning of Atomically Resolved Scanning Transmission Electron Microscopy Images: Chemical Identification and Tracking Local Transformations.

PubMed

Ziatdinov, Maxim; Dyck, Ondrej; Maksov, Artem; Li, Xufan; Sang, Xiahan; Xiao, Kai; Unocic, Raymond R; Vasudevan, Rama; Jesse, Stephen; Kalinin, Sergei V

2017-12-26

Recent advances in scanning transmission electron and scanning probe microscopies have opened exciting opportunities in probing the materials structural parameters and various functional properties in real space with angstrom-level precision. This progress has been accompanied by an exponential increase in the size and quality of data sets produced by microscopic and spectroscopic experimental techniques. These developments necessitate adequate methods for extracting relevant physical and chemical information from the large data sets, for which a priori information on the structures of various atomic configurations and lattice defects is limited or absent. Here we demonstrate an application of deep neural networks to extract information from atomically resolved images including location of the atomic species and type of defects. We develop a "weakly supervised" approach that uses information on the coordinates of all atomic species in the image, extracted via a deep neural network, to identify a rich variety of defects that are not part of an initial training set. We further apply our approach to interpret complex atomic and defect transformation, including switching between different coordination of silicon dopants in graphene as a function of time, formation of peculiar silicon dimer with mixed 3-fold and 4-fold coordination, and the motion of molecular "rotor". This deep learning-based approach resembles logic of a human operator, but can be scaled leading to significant shift in the way of extracting and analyzing information from raw experimental data.

Impact Flash Monitoring Facility on the Deep Space Gateway

NASA Astrophysics Data System (ADS)

Needham, D. H.; Moser, D. E.; Suggs, R. M.; Cooke, W. J.; Kring, D. A.; Neal, C. R.; Fassett, C. I.

2018-02-01

Cameras mounted to the Deep Space Gateway exterior will detect flashes caused by impacts on the lunar surface. Observed flashes will help constrain the current lunar impact flux and assess hazards faced by crews living and working in cislunar space.

Probing below the Surface of Mars. ITEA/NASA-JPL Learning Activity.

ERIC Educational Resources Information Center

Urquhart, Mary; Urquhart, Sally

2000-01-01

This activity, developed by NASA's Jet Propulsion Laboratory, involves students in recording and graphing temperature data to learn about NASA's Mars Microprobe Mission, Deep Space 2, and how the properties of a material affect the transfer of heat. (Author/JOW)

Non-invasive imaging and cellular tracking of pulmonary emboli by near-infrared fluorescence and positron-emission tomography

PubMed Central

Page, Michael J.; Lourenço, André L.; David, Tovo; LeBeau, Aaron M.; Cattaruzza, Fiore; Castro, Helena C.; VanBrocklin, Henry F.; Coughlin, Shaun R.; Craik, Charles S.

2015-01-01

Functional imaging of proteolytic activity is an emerging strategy to quantify disease and response to therapy at the molecular level. We present a new peptide-based imaging probe technology that advances these goals by exploiting enzymatic activity to deposit probes labelled with near-infrared (NIR) fluorophores or radioisotopes in cell membranes of disease-associated proteolysis. This strategy allows for non-invasive detection of protease activity in vivo and ex vivo by tracking deposited probes in tissues. We demonstrate non-invasive detection of thrombin generation in a murine model of pulmonary embolism using our protease-activated peptide probes in microscopic clots within the lungs with NIR fluorescence optical imaging and positron-emission tomography. Thrombin activity is imaged deep in tissue and tracked predominantly to platelets within the lumen of blood vessels. The modular design of our probes allows for facile investigation of other proteases, and their contributions to disease by tailoring the protease activation and cell-binding elements. PMID:26423607

Optimal surveys for weak-lensing tomography

NASA Astrophysics Data System (ADS)

Amara, Adam; Réfrégier, Alexandre

2007-11-01

Weak-lensing surveys provide a powerful probe of dark energy through the measurement of the mass distribution of the local Universe. A number of ground-based and space-based surveys are being planned for this purpose. Here, we study the optimal strategy for these future surveys using the joint constraints on the equation-of-state parameter wn and its evolution wa as a figure of merit by considering power spectrum tomography. For this purpose, we first consider an `ideal' survey which is both wide and deep and exempt from systematics. We find that such a survey has great potential for dark energy studies, reaching 1σ precisions of 1 and 10 per cent on the two parameters, respectively. We then study the relative impact of various limitations by degrading this ideal survey. In particular, we consider the effect of sky coverage, survey depth, shape measurement systematics, photometric redshift systematics and uncertainties in the non-linear power spectrum predictions. We find that, for a given observing time, it is always advantageous to choose a wide rather than a deep survey geometry. We also find that the dark energy constraints from power spectrum tomography are robust to photometric redshift errors and catastrophic failures, if a spectroscopic calibration sample of 104-105 galaxies are available. The impact of these systematics is small compared to the limitations that come from potential uncertainties in the power spectrum, due to shear measurement and theoretical errors. To help the planning of future surveys, we summarize our results with comprehensive scaling relations which avoid the need for full Fisher matrix calculations.

TH-AB-209-04: 3D Light Sheet Luminescence Imaging with Cherenkov Radiation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bruza, P; Lin, H; Jarvis, L

Purpose: To recover a three-dimensional density distribution of luminescent molecular probes located several centimeters deep within a highly scattering tissue. Methods: We developed a novel sheet beam Cherenkov-excited luminescence scanned imaging (CELSI) methodology. The sample was irradiated by a horizontally oriented, vertically scanned 6 MV X-ray sheet beam (200mm × 5mm, 0.2mm vertical step) from a radiotherapy linear accelerator. The resulting Cherenkov light emission – and thus luminescent probe excitation – occurred exclusively along the irradiation plane due to a short diffusion path of secondary particles and Cherenkov photons. Cherenkov-excited luminescence was detected orthogonally to the sheet beam by gated,more » intensified charge coupled device camera. Analogously to light sheet microscopy, a series of luminescence images was taken for varied axial positions (depths) of the Cherenkov light sheet in sample. Knowledge of the excitation plane position allowed a 3D image stack deconvolution and depth-variant attenuation correction. The 3D image post-processing yielded a true spatial density distribution of luminescent molecules in highly scattering tissue. Results: We recovered a three-dimensional shape and position of 400 µL lesion-mimicking phantom tubes containing 25 µM solution of PtG4 molecular probe from 3 centimeter deep tissue-like media. The high sensitivity of CELSI also allowed resolving 100 micron capillaries of test solution. Functional information of partial oxygen pressure at the site of PtG4 molecular probe was recovered from luminescence lifetime CELSI. Finally, in-vivo sheet beam CELSI localized milimeter-sized PtG4-labelled tumor phantoms in multiple biological objects (hairless mice) from single scan. Conclusion: Presented sheet beam CELSI technique greatly extended the useful depth range of luminescence molecular imaging. More importantly, the light sheet microscopy approach was successfully adapted to CELSI, providing means to recover a completely attenuation-corrected 3D image of luminescent probe distribution. Gated CELSI acquisition yielded functional information of a spatially resolved oxygen concentration map of deep lying targets. This work was supported by NIH research grant R01CA109558 and R21EB017559, as well as by Pilot Grant Funds from the Norris Cotton Cancer Center.« less

Energy-Looping Nanoparticles: Harnessing Excited-State Absorption for Deep-Tissue Imaging.

PubMed

Levy, Elizabeth S; Tajon, Cheryl A; Bischof, Thomas S; Iafrati, Jillian; Fernandez-Bravo, Angel; Garfield, David J; Chamanzar, Maysamreza; Maharbiz, Michel M; Sohal, Vikaas S; Schuck, P James; Cohen, Bruce E; Chan, Emory M

2016-09-27

Near infrared (NIR) microscopy enables noninvasive imaging in tissue, particularly in the NIR-II spectral range (1000-1400 nm) where attenuation due to tissue scattering and absorption is minimized. Lanthanide-doped upconverting nanocrystals are promising deep-tissue imaging probes due to their photostable emission in the visible and NIR, but these materials are not efficiently excited at NIR-II wavelengths due to the dearth of lanthanide ground-state absorption transitions in this window. Here, we develop a class of lanthanide-doped imaging probes that harness an energy-looping mechanism that facilitates excitation at NIR-II wavelengths, such as 1064 nm, that are resonant with excited-state absorption transitions but not ground-state absorption. Using computational methods and combinatorial screening, we have identified Tm(3+)-doped NaYF4 nanoparticles as efficient looping systems that emit at 800 nm under continuous-wave excitation at 1064 nm. Using this benign excitation with standard confocal microscopy, energy-looping nanoparticles (ELNPs) are imaged in cultured mammalian cells and through brain tissue without autofluorescence. The 1 mm imaging depths and 2 μm feature sizes are comparable to those demonstrated by state-of-the-art multiphoton techniques, illustrating that ELNPs are a promising class of NIR probes for high-fidelity visualization in cells and tissue.

Review—hexagonal boron nitride epilayers: Growth, optical properties and device applications

DOE PAGES

Jiang, H. X.; Lin, Jing Yu

2016-09-07

This paper provides a brief overview on recent advances made in authors’ laboratory in epitaxial growth and optical studies of hexagonal boron nitride (h-BN) epilayers and heterostructures. Photoluminescence spectroscopy has been employed to probe the optical properties of h-BN. It was observed that the near band edge emission of h-BN is unusually high and is more than two orders of magnitude higher than that of high quality AlN epilayers. It was shown that the unique quasi-2D nature induced by the layered structure of h-BN results in high optical absorption and emission. The impurity related and near band-edge transitions in h-BNmore » epilayers were probed for materials synthesized under varying ammonia flow rates. Our results have identified that the most dominant impurities and deep level defects in h-BN epilayers are related to nitrogen vacancies. By growing h-BN under high ammonia flow rates, nitrogen vacancy related defects can be eliminated and epilayers exhibiting pure free exciton emission have been obtained. Deep UV and thermal neutron detectors based on h-BN epilayers were shown to possess unique features. Lastly, it is our belief that h-BN will lead to many potential applications from deep UV emitters and detectors, radiation detectors, to novel 2D photonic and electronic devices.« less

Review—hexagonal boron nitride epilayers: Growth, optical properties and device applications

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jiang, H. X.; Lin, Jing Yu

This paper provides a brief overview on recent advances made in authors’ laboratory in epitaxial growth and optical studies of hexagonal boron nitride (h-BN) epilayers and heterostructures. Photoluminescence spectroscopy has been employed to probe the optical properties of h-BN. It was observed that the near band edge emission of h-BN is unusually high and is more than two orders of magnitude higher than that of high quality AlN epilayers. It was shown that the unique quasi-2D nature induced by the layered structure of h-BN results in high optical absorption and emission. The impurity related and near band-edge transitions in h-BNmore » epilayers were probed for materials synthesized under varying ammonia flow rates. Our results have identified that the most dominant impurities and deep level defects in h-BN epilayers are related to nitrogen vacancies. By growing h-BN under high ammonia flow rates, nitrogen vacancy related defects can be eliminated and epilayers exhibiting pure free exciton emission have been obtained. Deep UV and thermal neutron detectors based on h-BN epilayers were shown to possess unique features. Lastly, it is our belief that h-BN will lead to many potential applications from deep UV emitters and detectors, radiation detectors, to novel 2D photonic and electronic devices.« less

An Infrared Actin Probe for Deep-Cell Electroporation-Based Single-Molecule Speckle (eSiMS) Microscopy

PubMed Central

Yamashiro, Sawako; Watanabe, Naoki

2017-01-01

Single-molecule speckle (SiMS) microscopy is a powerful method to directly elucidate biochemical reactions in live cells. However, since the signal from an individual fluorophore is extremely faint, the observation area by epi-fluorescence microscopy is restricted to the thin cell periphery to reduce autofluorescence, or only molecules near the plasma membrane are visualized by total internal reflection fluorescence (TIRF) microscopy. Here, we introduce a new actin probe labeled with near infrared (NIR) emissive CF680R dye for easy-to-use, electroporation-based SiMS microscopy (eSiMS) for deep-cell observation. CF680R-labeled actin (CF680R-actin) incorporated into actin structures and showed excellent brightness and photostability suitable for single-molecule imaging. Importantly, the intensity of autofluorescence with respect to SiMS brightness was reduced to approximately 13% compared to DyLight 550-labeled actin (DL550-actin). CF680R-actin enabled the monitoring of actin SiMS in actomyosin bundles associated with adherens junctions (AJs) located at 3.5–4 µm above the basal surfaces of epithelial monolayers. These favorable properties of CF680R-actin extend the application of eSiMS to actin turnover and flow analyses in deep cellular structures. PMID:28671584

The criterial optics of oceans and glaciers with technogenic pollutions

NASA Astrophysics Data System (ADS)

Merzlikin, V. G.; Ilushin, Ya. A.; Olenin, A. L.; Sidorov, O. V.; Tovstonog, V. A.

2017-02-01

Effective diagnostics of natural and technogenic pollutions of the ocean and forming snow-ice cover is considered on the basis of priority observation and registration of the changing optical characteristics of the seawater and glaciers. The paper discusses Influence of abnormal optical properties on overheating of the seawater subsurface layer and appearance of significant irradiated oceanic deep horizons up to 100 m. Additional heating of atmosphere, strengthening of hurricanes during a storm, tornadogenesis, generation of dehydrated convective air flows at a calm and effect of overcooling deep seawater is analyzed using the scheme of calculated heat budget and temperature distributions under combined solar and atmospheric exposure. The authors propose to use their unique deep hydrological multi-channel probe for synchronous and independent registration of optical, temperature and other standard hydro physical characteristics developed by Shirshov Institute of Oceanology. The paper presents calculation algorithm of real variability of spatial and temporal temperature field due to influence of registered concentration field of foreign substances in the seawater irrespective of its hydrodynamic conditions. Inphase or antiphase changes of fixed temperature gradients and transparency for polluted seawater has been explained as the result of the various contributions of scattering and absorption within attenuation processes of probing radiation for the local volume at a specified depth.

Parametrization and evaluation of marine environmental impacts produced by deep-sea manganese nodule mining

NASA Astrophysics Data System (ADS)

Oebius, Horst U.; Becker, Hermann J.; Rolinski, Susanne; Jankowski, Jacek A.

The evaluation of marine environmental impacts resulting from the exploitation of marine resources requires the numerical description, parametrization, and modelling of such processes in order to be able to transfer, compare, and forecast the effects of anthropogenic activities in the deep sea. One of the controversial effects is the formation and behaviour of sediment clouds as a consequence of anthropogenic activities on the seafloor. Since there is a need for reliable data, two subprojects of the "Interdisciplinary Deep-sea Environmental Protection Group (TUSCH)"-project "Impacts from Technical Activities on the Deep-Sea Ecosystem of the South East Pacific Offshore Peru (ATESEPP)" were devoted to the assembly of such data. Based on the German technical approach for deep-sea mining, the possible environmental impacts by a miner were estimated, the impacts on the seafloor were simulated and investigated by tests with large volume undisturbed sediment samples on board the research vessel and in the laboratory, and the results were evaluated and extrapolated. This report gives a comprehensive presentation of the physical problems, the technical approach, and the results of these investigations.

Sedimentary Markers : a window into deep geodynamic processes Examples from the Western Mediterranean Sea

NASA Astrophysics Data System (ADS)

Rabineau, Marina; Aslanian, Daniel; Leroux, Estelle; Pellen, Romain; Gorini, Christian; Moulin, Maryline; Droz, Laurence; Bache, Francois; Molliex, Stephane; Silenzario, Carmine; Rubino, Jean-Loup

2017-04-01

Deep Earth dynamics impact so strongly on surface geological processes that we can use sediment palaeo-markers as a window into the deeper Earth. Derived from climatic and tectonic erosive actions on the continents, and related to eustasy, subsidence and isostasy, the sediment in a deep basin is the main recorder of these processes. Nevertheless, defining and quantifying the relative roles of parameters that interact to give the final sedimentary architecture is not a simple task. Using a 3D-grid of seismic and wide-angle data, boreholes and numerical stratigraphic modelling, we propose here a quantification of post-rift vertical movements in the Provençal Basin (Western Mediterranean) involving three domains of subsidence: seaward tilting on the platform and the slope and purely vertical subsidence in the deep basin (Rabineau et al., 2014 ; Leroux et al., 2015). These domains fit the deeper crustal domains highlighted by previous geophysical data (Moulin et al., 2015 ; Afilhado et al., 2015). Post-break-up sedimentary markers may therefore be used to identify the initial hinge lines of the rifting phase, to quantify sedimentation rates and isostatic rebound (Rabineau et al., 2014) and redefine the subsidence laws. Similar work and results are obtained in the Valencia Basin (Pellen et al., 2016). This Western Mediterranean Sea is a natural laboratory with very high total subsidence rates that enable high sedimentation rates along the margin with sediments provided by the Rhône and Ebro rivers flowing from the Alps, the Pyrennees and Catalan chains, which in turn archives the detailed record of climate/tectonic evolution during the Neogene. The Western Mediterranean Sea could therefore further probe deep-earth and surface connections using deep drillings of this land-locked ocean basin transformed into a giant saline basin (Rabineau et al., 2015). Leroux, E., Aslanian, D., Rabineau, M., M. Moulin, D. Granjeon, C. Gorini, L. Droz, 2015. Sedimentary markers: a window to deep geodynamic processes. Terra Nova 27, 122-129. Moulin, M., Klingelhoefer, F., Afilhado, A., Feld, A., Aslanian, D., Schnurle, P., Nouzé, H., Rabineau, M. & Beslier, M.O., 2015. Deep crustal structure across an young passive margin from wide- angle and reflection seismic date (The SARDINIA Experiment) - I- Gulf of Lion's Margin BSGF, ILP Special Volume, 186 (4-5), pp. 309-330 Afilhado A., M. Moulin, F. Klingelhoefer, D. Aslanian, P. Schnurle, H. Nouzé, M. Rabineau & M.O. Beslier, 2015. Deep crustal structure across a young passive margin from wide- angle and reflection seismic data (The SARDINIA Experiment) - II. Sardinia's margin, BSGF, ILP Special Volume, 186 (4-5), p. 331-351 Pellen, R., Aslanian, D., Rabineau, M., Leroux, E., Gorini, C., Silenzario, C., Blanpied, C., Rubino, J-L., 2016. The Minorca Basin: a buffer zone between Valencia and Provençal Basins, Terra Nova, 28-4, p. 245-256. Rabineau, M., Leroux, E., Aslanian, D., Bache, F., Gorini, C., Moulin, M., Molliex, S., Droz, L., Dos Reis, T., Rubino, J-L., Olivet, J-L., 2014. Quantifying Subsidence and Isostasy using paleobathymetric markers : example from the Gulf of Lion, EPSL, vol. 288, p. 353- 366. http://dx.doi.org/10.1016/j.epsl.2013.11.059 Rabineau, M., S. Cloetingh, J. Kuroda, D. Aslanian, A Droxler, C. Gorini, D. Garcia-Castellanos, A. Moscariello, Y. Hello, E. Burov, F. Sierro, F. Lirer, F. Roure, P.A. Pezard, L. Matenco, Y. Mart, A. Camerlenghi, A. Tripati and the GOLD and DREAM Working Groups, 2015. Probing connections between deep earth and surface processes in a land-locked ocean basin transformed into a giant saline basin: the Mediterranean GOLD project, Marine and Petroleum Geology, Volume: 66 Pages: 6-17.

Amateur Radio Communications with a Deep Space Probe (Yes, It's Possible)

NASA Astrophysics Data System (ADS)

Cudnik, Brian; Rahman, Mahmudur; Saganti, Seth; Erickson, Gary M.; Saganti, Premkumar

2015-05-01

Prairie View A&M University through the collaboration with NASA-Johnson Space Center has partnered with the Kyushu Institute of Technology (KIT), Japan and developed a payload for the Shinen-2 spacecraft that was launched from Japan on December 3, 2014 as part of the Hayabusa2 mission. The main purpose of the Shinen-2 spacecraft is deep space communication experiment to test the feasibility of deep-space radio communications from the spacecraft to Earth without the need of the Deep Space Network (DSN) of NASA. This presents an opportunity to the wider community of amateur astronomers, ham radio operators, and other research personnel in that they will have the opportunity to work with deep space communication such as Shinen-2 spacecraft. It should be possible to detect a signal as an increased strength from Shinen-2 spacecraft at a rest frequency of 437.385 MHz, using commercially available equipment procured at low-cost, when the spacecraft approaches to within 3,000,000 km of the Earth during December 2015.

Optical ground station site diversity for Deep Space Optical Communications the Mars Telecom Orbiter optical link

NASA Technical Reports Server (NTRS)

Wilson, K.; Parvin, B.; Fugate, R.; Kervin, P.; Zingales, S.

2003-01-01

Future NASA deep space missions will fly advanced high resolution imaging instruments that will require high bandwidth links to return the huge data volumes generated by these instruments. Optical communications is a key technology for returning these large data volumes from deep space probes. Yet to cost effectively realize the high bandwidth potential of the optical link will require deployment of ground receivers in diverse locations to provide high link availability. A recent analysis of GOES weather satellite data showed that a network of ground stations located in Hawaii and the Southwest continental US can provide an average of 90% availability for the deep space optical link. JPL and AFRL are exploring the use of large telescopes in Hawaii, California, and Albuquerque to support the Mars Telesat laser communications demonstration. Designed to demonstrate multi-Mbps communications from Mars, the mission will investigate key operational strategies of future deep space optical communications network.

Ecological impacts of large-scale disposal of mining waste in the deep sea

PubMed Central

Hughes, David J.; Shimmield, Tracy M.; Black, Kenneth D.; Howe, John A.

2015-01-01

Deep-Sea Tailings Placement (DSTP) from terrestrial mines is one of several large-scale industrial activities now taking place in the deep sea. The scale and persistence of its impacts on seabed biota are unknown. We sampled around the Lihir and Misima island mines in Papua New Guinea to measure the impacts of ongoing DSTP and assess the state of benthic infaunal communities after its conclusion. At Lihir, where DSTP has operated continuously since 1996, abundance of sediment infauna was substantially reduced across the sampled depth range (800–2020 m), accompanied by changes in higher-taxon community structure, in comparison with unimpacted reference stations. At Misima, where DSTP took place for 15 years, ending in 2004, effects on community composition persisted 3.5 years after its conclusion. Active tailings deposition has severe impacts on deep-sea infaunal communities and these impacts are detectable at a coarse level of taxonomic resolution. PMID:25939397

Ecological impacts of large-scale disposal of mining waste in the deep sea.

PubMed

Hughes, David J; Shimmield, Tracy M; Black, Kenneth D; Howe, John A

2015-05-05

Deep-Sea Tailings Placement (DSTP) from terrestrial mines is one of several large-scale industrial activities now taking place in the deep sea. The scale and persistence of its impacts on seabed biota are unknown. We sampled around the Lihir and Misima island mines in Papua New Guinea to measure the impacts of ongoing DSTP and assess the state of benthic infaunal communities after its conclusion. At Lihir, where DSTP has operated continuously since 1996, abundance of sediment infauna was substantially reduced across the sampled depth range (800-2020 m), accompanied by changes in higher-taxon community structure, in comparison with unimpacted reference stations. At Misima, where DSTP took place for 15 years, ending in 2004, effects on community composition persisted 3.5 years after its conclusion. Active tailings deposition has severe impacts on deep-sea infaunal communities and these impacts are detectable at a coarse level of taxonomic resolution.

An optical clearing technique for plant tissues allowing deep imaging and compatible with fluorescence microscopy.

PubMed

Warner, Cherish A; Biedrzycki, Meredith L; Jacobs, Samuel S; Wisser, Randall J; Caplan, Jeffrey L; Sherrier, D Janine

2014-12-01

We report on a nondestructive clearing technique that enhances transmission of light through specimens from diverse plant species, opening unique opportunities for microscope-enabled plant research. After clearing, plant organs and thick tissue sections are amenable to deep imaging. The clearing method is compatible with immunocytochemistry techniques and can be used in concert with common fluorescent probes, including widely adopted protein tags such as GFP, which has fluorescence that is preserved during the clearing process. © 2014 American Society of Plant Biologists. All Rights Reserved.

Integrating Concepts in Biology Textbook Increases Learning: Assessment Triangulation Using Concept Inventory, Card Sorting, and MCAT Instruments, Followed by Longitudinal Tracking

PubMed Central

Luckie, Douglas B.; Hoskinson, Anne-Marie; Griffin, Caleigh E.; Hess, Andrea L.; Price, Katrina J.; Tawa, Alex; Thacker, Samantha M.

2017-01-01

The purpose of this study was to examine the educational impact of an intervention, the inquiry-focused textbook Integrating Concepts in Biology (ICB), when used in a yearlong introductory biology course sequence. Student learning was evaluated using three published instruments: 1) The Biology Concept Inventory probed depth of student mastery of fundamental concepts in organismal and cellular topics when confronting misconceptions as distractors. ICB students had higher gains in all six topic categories (+43% vs. peers overall, p < 0.01). 2) The Biology Card Sorting Task assessed whether students organized biological ideas more superficially, as novices do, or based on deeper concepts, like experts. The frequency with which ICB students connected deep-concept pairs, or triplets, was similar to peers; but deep understanding of structure/function was much higher (for pairs: 77% vs. 25%, p < 0.01). 3) A content-focused Medical College Admission Test (MCAT) posttest compared ICB student content knowledge with that of peers from 15 prior years. Historically, MCAT performance for each semester ranged from 53% to 64%; the ICB cohort scored 62%, in the top quintile. Longitudinal tracking in five upper-level science courses the following year found ICB students outperformed peers in physiology (85% vs. 80%, p < 0.01). PMID:28389429

Photoswitchable non-fluorescent thermochromic dye-nanoparticle hybrid probes.

PubMed

Harrington, Walter N; Haji, Mwafaq R; Galanzha, Ekaterina I; Nedosekin, Dmitry A; Nima, Zeid A; Watanabe, Fumiya; Ghosh, Anindya; Biris, Alexandru S; Zharov, Vladimir P

2016-11-08

Photoswitchable fluorescent proteins with controllable light-dark states and spectral shifts in emission in response to light have led to breakthroughs in the study of cell biology. Nevertheless, conventional photoswitching is not applicable for weakly fluorescent proteins and requires UV light with low depth penetration in bio-tissue. Here we introduce a novel concept of photoswitchable hybrid probes consisting of thermochromic dye and absorbing nanoparticles, in which temperature-sensitive light-dark states and spectral shifts in absorption can be switched through controllable photothermal heating of doped nanoparticles. The proof-of-concept is demonstrated through the use of two different types of temperature-sensitive dyes doped with magnetic nanoparticles and reversibly photoswitched by a near-infrared laser. Photoacoustic imaging revealed the high contrast of these probes, which is sufficient for their visualization in cells and deep tissue. Our results suggest that these new photoswitchable multicolour probes can be used for multimodal cellular diagnostics and potentially for magnetic and photothermal therapy.

Photoswitchable non-fluorescent thermochromic dye-nanoparticle hybrid probes

NASA Astrophysics Data System (ADS)

Harrington, Walter N.; Haji, Mwafaq R.; Galanzha, Ekaterina I.; Nedosekin, Dmitry A.; Nima, Zeid A.; Watanabe, Fumiya; Ghosh, Anindya; Biris, Alexandru S.; Zharov, Vladimir P.

2016-11-01

Photoswitchable fluorescent proteins with controllable light-dark states and spectral shifts in emission in response to light have led to breakthroughs in the study of cell biology. Nevertheless, conventional photoswitching is not applicable for weakly fluorescent proteins and requires UV light with low depth penetration in bio-tissue. Here we introduce a novel concept of photoswitchable hybrid probes consisting of thermochromic dye and absorbing nanoparticles, in which temperature-sensitive light-dark states and spectral shifts in absorption can be switched through controllable photothermal heating of doped nanoparticles. The proof-of-concept is demonstrated through the use of two different types of temperature-sensitive dyes doped with magnetic nanoparticles and reversibly photoswitched by a near-infrared laser. Photoacoustic imaging revealed the high contrast of these probes, which is sufficient for their visualization in cells and deep tissue. Our results suggest that these new photoswitchable multicolour probes can be used for multimodal cellular diagnostics and potentially for magnetic and photothermal therapy.

Pico Reentry Probes: Affordable Options for Reentry Measurements and Testing

NASA Technical Reports Server (NTRS)

Ailor, William H.; Kapoor, Vinod B.; Allen, Gay A., Jr.; Venkatapathy, Ethiraj; Arnold, James O.; Rasky, Daniel J.

2005-01-01

It is generally very costly to perform in-space and atmospheric entry experiments. This paper presents a new platform - the Pico Reentry Probe (PREP) - that we believe will make targeted flight-tests and planetary atmospheric probe science missions considerably more affordable. Small, lightweight, self-contained, it is designed as a "launch and forget" system, suitable for experiments that require no ongoing communication with the ground. It contains a data recorder, battery, transmitter, and user-customized instrumentation. Data recorded during reentry or space operations is returned at end-of-mission via transmission to Iridium satellites (in the case of earth-based operations) or a similar orbiting communication system for planetary missions. This paper discusses possible applications of this concept for Earth and Martian atmospheric entry science. Two well-known heritage aerodynamic shapes are considered as candidates for PREP: the shape developed for the Planetary Atmospheric Experiment Test (PAET) and that for the Deep Space II Mars Probe.

In-situ investigation of protein and DNA structure using UVRRS

NASA Astrophysics Data System (ADS)

Greek, L. Shane; Schulze, H. Georg; Blades, Michael W.; Haynes, Charles A.; Turner, Robin F. B.

1997-05-01

Ultraviolet resonance Raman spectroscopy (UVRRS) has the potential to become a sensitive, specific, versatile bioanalytical and biophysical technique for routine investigations of proteins, DNA, and their monomeric components, as well as a variety smaller, physiologically important aromatic molecules. The transition of UVRRS from a complex, specialized spectroscopic method to a common laboratory assay depends upon several developments, including a robust sample introduction method permitting routine, in situ analysis in standard laboratory environments. To this end, we recently reported the first fiber-optic probes suitable for deep-UV pulsed laser UVRRS. In this paper, we extend this work by demonstrating the applicability of such probes to studies of biochemical relevance, including investigations of the resonance enhancement of phosphotyrosine, thermal denaturation of RNase T1, and specific and non-specific protein binding. The advantages and disadvantages of the probes are discussed with reference to sample conditions and probe design considerations.

Optogenetic interrogation of neural circuits: technology for probing mammalian brain structures

PubMed Central

Zhang, Feng; Gradinaru, Viviana; Adamantidis, Antoine R; Durand, Remy; Airan, Raag D; de Lecea, Luis; Deisseroth, Karl

2015-01-01

Elucidation of the neural substrates underlying complex animal behaviors depends on precise activity control tools, as well as compatible readout methods. Recent developments in optogenetics have addressed this need, opening up new possibilities for systems neuroscience. Interrogation of even deep neural circuits can be conducted by directly probing the necessity and sufficiency of defined circuit elements with millisecond-scale, cell type-specific optical perturbations, coupled with suitable readouts such as electrophysiology, optical circuit dynamics measures and freely moving behavior in mammals. Here we collect in detail our strategies for delivering microbial opsin genes to deep mammalian brain structures in vivo, along with protocols for integrating the resulting optical control with compatible readouts (electrophysiological, optical and behavioral). The procedures described here, from initial virus preparation to systems-level functional readout, can be completed within 4–5 weeks. Together, these methods may help in providing circuit-level insight into the dynamics underlying complex mammalian behaviors in health and disease. PMID:20203662

Deep-Red Fluorescent Gold Nanoclusters for Nucleoli Staining: Real-Time Monitoring of the Nucleolar Dynamics in Reverse Transformation of Malignant Cells.

PubMed

Wang, Xiaojuan; Wang, Yanan; He, Hua; Ma, Xiqi; Chen, Qi; Zhang, Shuai; Ge, Baosheng; Wang, Shengjie; Nau, Werner M; Huang, Fang

2017-05-31

Nucleoli are important subnuclear structures inside cells. We report novel fluorescent gold nanoclusters (K-AuNCs) that are able to stain the nucleoli selectively and make it possible to explore the nucleolar morphology with fluorescence imaging technique. This novel probe is prepared through an easy synthesis method by employing a tripeptide (Lys-Cys-Lys) as the surface ligand. The properties, including deep-red fluorescence emission (680 nm), large Stocks shift, broad excitation band, low cytotoxicity, and good photostability, endow this probe with potential for bioanalytical applications. Because of their small size and their positively charged surface, K-AuNCs are able to accumulate efficiently at the nucleolar regions and provide precise morphological information. K-AuNCs are also used to monitor the nucleolar dynamics along the reverse-transformation process of malignant cells, induced by the agonist of protein A, 8-chloro-cyclic adenosine monophosphate. This gives a novel approach for investigating the working mechanism of antitumor drugs.

Technique Developed for Optimizing Traveling-Wave Tubes

NASA Technical Reports Server (NTRS)

Wilson, Jeffrey D.

1999-01-01

A traveling-wave tube (TWT) is an electron beam device that is used to amplify electromagnetic communication waves at radio and microwave frequencies. TWT s are critical components in deep-space probes, geosynchronous communication satellites, and high-power radar systems. Power efficiency is of paramount importance for TWT s employed in deep-space probes and communications satellites. Consequently, increasing the power efficiency of TWT s has been the primary goal of the TWT group at the NASA Lewis Research Center over the last 25 years. An in-house effort produced a technique (ref. 1) to design TWT's for optimized power efficiency. This technique is based on simulated annealing, which has an advantage over conventional optimization techniques in that it enables the best possible solution to be obtained (ref. 2). A simulated annealing algorithm was created and integrated into the NASA TWT computer model (ref. 3). The new technique almost doubled the computed conversion power efficiency of a TWT from 7.1 to 13.5 percent (ref. 1).

Earth's gravity field to the eighteenth degree and geocentric coordinates for 104 stations from satellite and terrestrial data

NASA Technical Reports Server (NTRS)

Gaposchkin, E. M.

1973-01-01

Geodetic parameters describing the earth's gravity field and the positions of satellite-tracking stations in a geocentric reference frame were computed. These parameters were estimated by means of a combination of five different types of data: routine and simultaneous satellite observations, observations of deep-space probes, measurements of terrestrial gravity, and surface-triangulation data. The combination gives better parameters than does any subset of data types. The dynamic solution used precision-reduced Baker-Nunn observations and laser range data of 25 satellites. Data from the 49-station National Oceanic and Atmospheric Administration BC-4 network, the 19-station Smithsonian Astrophysical Observatory Baker-Nunn network, and independent camera stations were employed in the geometrical solution. Data from the tracking of deep-space probes were converted to relative longitudes and distances to the earth's axis of rotation of the tracking stations. Surface-gravity data in the form of 550-km squares were derived from 19,328 1 deg X 1 deg mean gravity anomalies.

Modulation of chorus intensity by ULF waves deep in the inner magnetosphere

DOE PAGES

Xia, Zhiyang; Chen, Lunjin; Dai, Lei; ...

2016-09-05

Previous studies have shown that chorus wave intensity can be modulated by Pc4-Pc5 compressional ULF waves. In this paper, we present Van Allen Probes observation of ULF wave modulating chorus wave intensity, which occurred deep in the magnetosphere. The ULF wave shows fundamental poloidal mode signature and mirror mode compressional nature. The observed ULF wave can modulate not only the chorus wave intensity but also the distribution of both protons and electrons. Linear growth rate analysis shows consistence with observed chorus intensity variation at low frequency (f <~ 0.3f ce), but cannot account for the observed higher-frequency chorus waves, includingmore » the upper band chorus waves. This suggests the chorus waves at higher-frequency ranges require nonlinear mechanisms. Finally, in addition, we use combined observations of Radiation Belt Storm Probes (RBSP) A and B to verify that the ULF wave event is spatially local and does not last long.« less

Receivers

NASA Technical Reports Server (NTRS)

Donnelly, H.

1983-01-01

Before discussing Deep Space Network receivers, a brief description of the functions of receivers and how they interface with other elements of the Network is presented. Different types of receivers are used in the Network for various purposes. The principal receiver type is used for telemetry and tracking. This receiver provides the capability, with other elements of the Network, to track the space probe utilizing Doppler and range measurements, and to receive telemetry, including both scientific data from the onboard experiments and engineering data pertaining to the health of the probe. Another type of receiver is used for radio science applications. This receiver measures phase perturbations on the carrier signal to obtain information on the composition of solar and planetary atmospheres and interplanetary space. A third type of receiver utilizes very long baseline interferometry (VLBI) techniques for both radio science and spacecraft navigation data. Only the telemetry receiver is described in detail in this document. The integration of the Receiver-Exciter subsystem with other portions of the Deep Space Network is described.

Focal switching of photochromic fluorescent proteins enables multiphoton microscopy with superior image contrast.

PubMed

Kao, Ya-Ting; Zhu, Xinxin; Xu, Fang; Min, Wei

2012-08-01

Probing biological structures and functions deep inside live organisms with light is highly desirable. Among the current optical imaging modalities, multiphoton fluorescence microscopy exhibits the best contrast for imaging scattering samples by employing a spatially confined nonlinear excitation. However, as the incident laser power drops exponentially with imaging depth into the sample due to the scattering loss, the out-of-focus background eventually overwhelms the in-focus signal, which defines a fundamental imaging-depth limit. Herein we significantly improve the image contrast for deep scattering samples by harnessing reversibly switchable fluorescent proteins (RSFPs) which can be cycled between bright and dark states upon light illumination. Two distinct techniques, multiphoton deactivation and imaging (MPDI) and multiphoton activation and imaging (MPAI), are demonstrated on tissue phantoms labeled with Dronpa protein. Such a focal switch approach can generate pseudo background-free images. Conceptually different from wave-based approaches that try to reduce light scattering in turbid samples, our work represents a molecule-based strategy that focused on imaging probes.

Focal switching of photochromic fluorescent proteins enables multiphoton microscopy with superior image contrast

PubMed Central

Kao, Ya-Ting; Zhu, Xinxin; Xu, Fang; Min, Wei

2012-01-01

Probing biological structures and functions deep inside live organisms with light is highly desirable. Among the current optical imaging modalities, multiphoton fluorescence microscopy exhibits the best contrast for imaging scattering samples by employing a spatially confined nonlinear excitation. However, as the incident laser power drops exponentially with imaging depth into the sample due to the scattering loss, the out-of-focus background eventually overwhelms the in-focus signal, which defines a fundamental imaging-depth limit. Herein we significantly improve the image contrast for deep scattering samples by harnessing reversibly switchable fluorescent proteins (RSFPs) which can be cycled between bright and dark states upon light illumination. Two distinct techniques, multiphoton deactivation and imaging (MPDI) and multiphoton activation and imaging (MPAI), are demonstrated on tissue phantoms labeled with Dronpa protein. Such a focal switch approach can generate pseudo background-free images. Conceptually different from wave-based approaches that try to reduce light scattering in turbid samples, our work represents a molecule-based strategy that focused on imaging probes. PMID:22876358

Piezoelectric modulation of surface voltage in GaN and AlGaN/GaN: charge screening effects and 2DEG

NASA Astrophysics Data System (ADS)

Wilson, Marshall; Schrayer, Bret; Savtchouk, Alexandre; Hillard, Bob; Lagowski, Jacek

2017-02-01

Surface voltage response to pulses of piezoelectric polarization is measured with a Kelvin-probe providing a unique means for investigation of the dynamics of polarization induced sheet charge and 2DEG. Combined with biasing of the surface with a corona-deposited charge from accumulation to deep depletion and corresponding non-contact C-V type characterization, the technique identifies surface band bending and interface traps as key factors that affect the magnitude and time decay of piezoelectric polarization. For 2DEG structures, surface potential pinning is observed when the 2DEG is fully populated. Pinning is released by negative corona charging to fully deplete the 2DEG. These results are consistent with the role of surface states. Presently demonstrated polarization modulation and wafer scale measurements shall impact the in-depth characterization and fundamental understanding of AlGaN/GaN 2DEG structures.

Design and evaluation of an ultra-slim objective for in-vivo deep optical biopsy

PubMed Central

Landau, Sara M.; Liang, Chen; Kester, Robert T.; Tkaczyk, Tomasz S.; Descour, Michael R.

2010-01-01

An estimated 1.6 million breast biopsies are performed in the US each year. In order to provide real-time, in-vivo imaging with sub-cellular resolution for optical biopsies, we have designed an ultra-slim objective to fit inside the 1-mm-diameter hypodermic needles currently used for breast biopsies to image tissue stained by the fluorescent probe proflavine. To ensure high-quality imaging performance, experimental tests were performed to characterize fiber bundle’s light-coupling efficiency and simulations were performed to evaluate the impact of candidate lens materials’ autofluorescence. A prototype of NA = 0.4, 250-µm field of view, ultra-slim objective optics was built and tested, yielding diffraction-limited performance and estimated resolution of 0.9 µm. When used in conjunction with a commercial coherent fiber bundle to relay the image formed by the objective, the measured resolution was 2.5 µm. PMID:20389489

KSC-05PD-0139

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. In the Press Site Auditorium, NASA managers and employees listen to NASA Administrator Sean O'Keefe's report on the state of the Agency. The update was broadcast live via NASA Television. O'Keefe focused on the achievements of 2004 and the goals set for 2005. His remarks emphasized the milestones met in NASA's Vision for Space Exploration, including the launch of the comet-chasing Deep Impact mission and the landing of the Huygens probe on Jupiters moon Titan, both occurring in the past two days, and the progress made in meeting the requirements to return the Space Shuttle to flight. OKeefes briefing included a dialogue with Associate Administrator of NASAs Office of Exploration Systems Craig Steidle and Center Director Jim Kennedy, live; and Manager of the Space Station Office Bill Gerstenmaier and Director of Advanced Planning and Jet Propulsion Laboratory Charles Elachi, via satellite.

KSC-05PD-0142

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. NASA Administrator Sean OKeefe (right) is accompanied on stage in the Press Site Auditorium by Center Director Jim Kennedy for a report to employees on the state of the Agency. The update was broadcast live via NASA Television. O'Keefe focused on the achievements of 2004 and the goals set for 2005. His remarks emphasized the milestones met in NASA's Vision for Space Exploration, including the launch of the comet-chasing Deep Impact mission and the landing of the Huygens probe on Jupiters moon Titan, both occurring in the past two days, and the progress made in meeting the requirements to return the Space Shuttle to flight. OKeefes briefing included a dialogue with Associate Administrator of NASAs Office of Exploration Systems Craig Steidle and Center Director Jim Kennedy, live; and Manager of the Space Station Office Bill Gerstenmaier and Director of Advanced Planning and Jet Propulsion Laboratory Charles Elachi, via satellite.

KSC-05PD-0144

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. NASA Administrator Sean OKeefe (right) is accompanied on stage in the Press Site Auditorium by Center Director Jim Kennedy for a report to employees on the state of the Agency. The update was broadcast live via NASA Television. O'Keefe focused on the achievements of 2004 and the goals set for 2005. His remarks emphasized the milestones met in NASA's Vision for Space Exploration, including the launch of the comet-chasing Deep Impact mission and the landing of the Huygens probe on Jupiters moon Titan, both occurring in the past two days, and the progress made in meeting the requirements to return the Space Shuttle to flight. OKeefes briefing included a dialogue with Associate Administrator of NASAs Office of Exploration Systems Craig Steidle and Center Director Jim Kennedy, live; and Manager of the Space Station Office Bill Gerstenmaier and Director of Advanced Planning and Jet Propulsion Laboratory Charles Elachi, via satellite.

KSC-05PD-0145

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. NASA Administrator Sean OKeefe (right) is accompanied on stage in the Press Site Auditorium by Associate Administrator of NASAs Office of Exploration Systems Craig Steidle for a report to employees on the state of the Agency. The update was broadcast live via NASA Television. O'Keefe focused on the achievements of 2004 and the goals set for 2005. His remarks emphasized the milestones met in NASA's Vision for Space Exploration, including the launch of the comet-chasing Deep Impact mission and the landing of the Huygens probe on Jupiters moon Titan, both occurring in the past two days, and the progress made in meeting the requirements to return the Space Shuttle to flight. OKeefes briefing included a dialogue with Associate Administrator of NASAs Office of Exploration Systems Craig Steidle and Center Director Jim Kennedy, live; and Manager of the Space Station Office Bill Gerstenmaier and Director of Advanced Planning and Jet Propulsion Laboratory Charles Elachi, via satellite.

KSC-05PD-0146

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. NASA Administrator Sean OKeefe (right) is accompanied on stage in the Press Site Auditorium by Associate Administrator of NASAs Office of Exploration Systems Craig Steidle for a report to employees on the state of the Agency. The update was broadcast live via NASA Television. O'Keefe focused on the achievements of 2004 and the goals set for 2005. His remarks emphasized the milestones met in NASA's Vision for Space Exploration, including the launch of the comet-chasing Deep Impact mission and the landing of the Huygens probe on Jupiters moon Titan, both occurring in the past two days, and the progress made in meeting the requirements to return the Space Shuttle to flight. OKeefes briefing included a dialogue with Associate Administrator of NASAs Office of Exploration Systems Craig Steidle and Center Director Jim Kennedy, live; and Manager of the Space Station Office Bill Gerstenmaier and Director of Advanced Planning and Jet Propulsion Laboratory Charles Elachi, via satellite.

KSC-05PD-0143

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. NASA Administrator Sean OKeefe (right) is accompanied on stage in the Press Site Auditorium by Center Director Jim Kennedy for a report to employees on the state of the Agency. The update was broadcast live via NASA Television. O'Keefe focused on the achievements of 2004 and the goals set for 2005. His remarks emphasized the milestones met in NASA's Vision for Space Exploration, including the launch of the comet-chasing Deep Impact mission and the landing of the Huygens probe on Jupiters moon Titan, both occurring in the past two days, and the progress made in meeting the requirements to return the Space Shuttle to flight. OKeefes briefing included a dialogue with Associate Administrator of NASAs Office of Exploration Systems Craig Steidle and Center Director Jim Kennedy, live; and Manager of the Space Station Office Bill Gerstenmaier and Director of Advanced Planning and Jet Propulsion Laboratory Charles Elachi, via satellite.

Small but mighty: Dark matter substructures

NASA Astrophysics Data System (ADS)

Cyr-Racine, Francis-Yan; Keeton, Charles; Moustakas, Leonidas

2018-01-01

The fundamental properties of dark matter, such as its mass, self-interaction, and coupling to other particles, can have a major impact on the evolution of cosmological density fluctuations on small length scales. Strong gravitational lenses have long been recognized as powerful tools to study the dark matter distribution on these small subgalactic scales. In this talk, we discuss how gravitationally lensed quasars and extended lensed arcs could be used to probe non minimal dark matter models. We comment on the possibilities enabled by precise astrometry, deep imaging, and time delays to extract information about mass substructures inside lens galaxies. To this end, we introduce a new lensing statistics that allows for a robust diagnostic of the presence of perturbations caused by substructures. We determine which properties of mass substructures are most readily constrained by lensing data and forecast the constraining power of current and future observations.

Gravity Wave Interactions with Fine Structures in the Mesosphere and Lower Thermosphere

NASA Astrophysics Data System (ADS)

Mixa, Tyler; Fritts, David; Bossert, Katrina; Laughman, Brian; Wang, Ling; Lund, Thomas; Kantha, Lakshmi

2017-04-01

An anelastic numerical model is used to probe the influences of fine layering structures on gravity wave propagation in the Mesosphere and Lower Thermosphere (MLT). Recent lidar observations confirm the presence of persistent layered structures in the MLT that have sharp stratification and vertical scales below 1km. Gravity waves propagating through finely layered environments can excite and modulate the evolution of small scale instabilities that redefine the layering structure in these regions. Such layers in turn filter the outgoing wave spectra, promote ducting or reflection, hasten the onset of self-acceleration dynamics, and encourage wave/mean-flow interactions via energy and momentum transport. Using high resolution simulations of a localized gravity wave packet in a deep atmosphere, we identify the relative impacts of various wave and mean flow parameters to improve our understanding of these dynamics and complement recent state-of-the-art observations.

The economic implications of changing regulations for deep sea fishing under the European Common Fisheries Policy: UK case study.

PubMed

Mangi, Stephen C; Kenny, Andrew; Readdy, Lisa; Posen, Paulette; Ribeiro-Santos, Ana; Neat, Francis C; Burns, Finlay

2016-08-15

Economic impact assessment methodology was applied to UK fisheries data to better understand the implications of European Commission proposal for regulations to fishing for deep-sea stocks in the North-East Atlantic (EC COM 371 Final 2012) under the Common Fisheries Policy (CFP). The aim was to inform the on-going debate to develop the EC proposal, and to assist the UK fishing industry and Government in evaluating the most effective options to manage deep sea fish stocks. Results indicate that enforcing the EC proposal as originally drafted results in a number of implications for the UK fleet. Because of the proposed changes to the list of species defined as being deep sea species, and a new definition of what constitutes a vessel targeting deep sea species, a total of 695 active UK fishing vessels would need a permit to fish for deep sea species. However, due to existing and capped capacity limits many vessels would potentially not be able to obtain such a permit. The economic impact of these changes from the status quo reveals that in the short term, landings would decrease by 6540 tonnes, reducing gross value added by £3.3 million. Alternative options were also assessed that provide mitigation measures to offset the impacts of the proposed regulations whilst at the same time providing more effective protection of deep sea Vulnerable Marine Ecosystems (VMEs). The options include setting a 400m depth rule that identifies a depth beyond which vessels would potentially be classified as fishing for deep sea species and designating 'core areas' for deep sea fishing at depths>400m to minimise the risk of further impacts of bottom fishing gear on deep sea habitats. Applying a 400m depth limit and 'core fishing' area approach deeper than 400m, the impact of the EC proposal would essentially be reduced to zero, that is, on average no vessels (using the status quo capacity baseline) would be impacted by the proposal. Copyright © 2016 Elsevier B.V. All rights reserved.

Enriching mission planning approach with state transition graph heuristics for deep space exploration

NASA Astrophysics Data System (ADS)

Jin, Hao; Xu, Rui; Xu, Wenming; Cui, Pingyuan; Zhu, Shengying

2017-10-01

As to support the mission of Mars exploration in China, automated mission planning is required to enhance security and robustness of deep space probe. Deep space mission planning requires modeling of complex operations constraints and focus on the temporal state transitions of involved subsystems. Also, state transitions are ubiquitous in physical systems, but have been elusive for knowledge description. We introduce a modeling approach to cope with these difficulties that takes state transitions into consideration. The key technique we build on is the notion of extended states and state transition graphs. Furthermore, a heuristics that based on state transition graphs is proposed to avoid redundant work. Finally, we run comprehensive experiments on selected domains and our techniques present an excellent performance.

Impacts on the deep-sea ecosystem by a severe coastal storm.

PubMed

Sanchez-Vidal, Anna; Canals, Miquel; Calafat, Antoni M; Lastras, Galderic; Pedrosa-Pàmies, Rut; Menéndez, Melisa; Medina, Raúl; Company, Joan B; Hereu, Bernat; Romero, Javier; Alcoverro, Teresa

2012-01-01

Major coastal storms, associated with strong winds, high waves and intensified currents, and occasionally with heavy rains and flash floods, are mostly known because of the serious damage they can cause along the shoreline and the threats they pose to navigation. However, there is a profound lack of knowledge on the deep-sea impacts of severe coastal storms. Concurrent measurements of key parameters along the coast and in the deep-sea are extremely rare. Here we present a unique data set showing how one of the most extreme coastal storms of the last decades lashing the Western Mediterranean Sea rapidly impacted the deep-sea ecosystem. The storm peaked the 26(th) of December 2008 leading to the remobilization of a shallow-water reservoir of marine organic carbon associated with fine particles and resulting in its redistribution across the deep basin. The storm also initiated the movement of large amounts of coarse shelf sediment, which abraded and buried benthic communities. Our findings demonstrate, first, that severe coastal storms are highly efficient in transporting organic carbon from shallow water to deep water, thus contributing to its sequestration and, second, that natural, intermittent atmospheric drivers sensitive to global climate change have the potential to tremendously impact the largest and least known ecosystem on Earth, the deep-sea ecosystem.

Impacts on the Deep-Sea Ecosystem by a Severe Coastal Storm

PubMed Central

Sanchez-Vidal, Anna; Canals, Miquel; Calafat, Antoni M.; Lastras, Galderic; Pedrosa-Pàmies, Rut; Menéndez, Melisa; Medina, Raúl; Company, Joan B.; Hereu, Bernat; Romero, Javier; Alcoverro, Teresa

2012-01-01

Major coastal storms, associated with strong winds, high waves and intensified currents, and occasionally with heavy rains and flash floods, are mostly known because of the serious damage they can cause along the shoreline and the threats they pose to navigation. However, there is a profound lack of knowledge on the deep-sea impacts of severe coastal storms. Concurrent measurements of key parameters along the coast and in the deep-sea are extremely rare. Here we present a unique data set showing how one of the most extreme coastal storms of the last decades lashing the Western Mediterranean Sea rapidly impacted the deep-sea ecosystem. The storm peaked the 26th of December 2008 leading to the remobilization of a shallow-water reservoir of marine organic carbon associated with fine particles and resulting in its redistribution across the deep basin. The storm also initiated the movement of large amounts of coarse shelf sediment, which abraded and buried benthic communities. Our findings demonstrate, first, that severe coastal storms are highly efficient in transporting organic carbon from shallow water to deep water, thus contributing to its sequestration and, second, that natural, intermittent atmospheric drivers sensitive to global climate change have the potential to tremendously impact the largest and least known ecosystem on Earth, the deep-sea ecosystem. PMID:22295084

Deep seawater inherent optical properties in the Southern Ionian Sea

NASA Astrophysics Data System (ADS)

Riccobene, G.; Capone, A.; Aiello, S.; Ambriola, M.; Ameli, F.; Amore, I.; Anghinolfi, M.; Anzalone, A.; Avanzini, C.; Barbarino, G.; Barbarito, E.; Battaglieri, M.; Bellotti, R.; Beverini, N.; Bonori, M.; Bouhadef, B.; Brescia, M.; Cacopardo, G.; Cafagna, F.; Caponetto, L.; Castorina, E.; Ceres, A.; Chiarusi, T.; Circella, M.; Cocimano, R.; Coniglione, R.; Cordelli, M.; Costa, M.; Cuneo, S.; D'Amico, A.; de Bonis, G.; de Marzo, C.; de Rosa, G.; de Vita, R.; Distefano, C.; Falchini, E.; Fiorello, C.; Flaminio, V.; Fratini, K.; Gabrielli, A.; Galeotti, S.; Gandolfi, E.; Grimaldi, A.; Habel, R.; Leonora, E.; Lonardo, A.; Longo, G.; Lo Presti, D.; Lucarelli, F.; Maccioni, E.; Margiotta, A.; Martini, A.; Masullo, R.; Megna, R.; Migneco, E.; Mongelli, M.; Montaruli, T.; Morganti, M.; Musumeci, M.; Nicolau, C. A.; Orlando, A.; Osipenko, M.; Osteria, G.; Papaleo, R.; Pappalardo, V.; Petta, C.; Piattelli, P.; Raffaelli, F.; Raia, G.; Randazzo, N.; Reito, S.; Ricco, G.; Ripani, M.; Rovelli, A.; Ruppi, M.; Russo, G. V.; Russo, S.; Russo, S.; Sapienza, P.; Sedita, M.; Schuller, J.-P.; Shirokov, E.; Simeone, F.; Sipala, V.; Spurio, M.; Taiuti, M.; Terreni, G.; Trasatti, L.; Urso, S.; Valente, V.; Vicini, P.

2007-02-01

The NEMO (NEutrino Mediterranean Observatory) Collaboration has been carrying out since 1998 an evaluation programme of deep sea sites suitable for the construction of the future Mediterranean km3 Čerenkov neutrino telescope. We investigated the seawater optical and oceanographic properties of several deep sea marine areas close to the Italian Coast. Inherent optical properties (light absorption and attenuation coefficients) have been measured as a function of depth using an experimental apparatus equipped with standard oceanographic probes and the commercial transmissometer AC9 manufactured by WETLabs. This paper reports on the visible light absorption and attenuation coefficients measured in deep seawater of a marine region located in the Southern Ionian Sea, 60 100 km SE of Capo Passero (Sicily). Data show that blue light absorption coefficient is about 0.015 m-1 (corresponding to an absorption length of 67 m) close to the one of optically pure water and it does not show seasonal variation.

High Resolution PET Imaging Probe for the Detection, Molecular Characterization, and Treatment Monitoring of Prostate cancer

DTIC Science & Technology

2011-07-01

impact on overall probe performance, including spatial resolution, energy resolution and timing resolution. We will show that 1 mm3 voxels will...ring, description of a strategy to remove scattered events, followed by characterization of impact of timing properties of the probe. In summary, the...described by the total distance d¼d1+d2 and a¼ d1=ðd1þd2Þ. We also want to account for impact angles, y1 and y2, defined versus line perpendicular to

An Impacting Descent Probe for Europa and the Other Galilean Moons of Jupiter

NASA Astrophysics Data System (ADS)

Wurz, P.; Lasi, D.; Thomas, N.; Piazza, D.; Galli, A.; Jutzi, M.; Barabash, S.; Wieser, M.; Magnes, W.; Lammer, H.; Auster, U.; Gurvits, L. I.; Hajdas, W.

2017-08-01

We present a study of an impacting descent probe that increases the science return of spacecraft orbiting or passing an atmosphere-less planetary bodies of the solar system, such as the Galilean moons of Jupiter. The descent probe is a carry-on small spacecraft (<100 kg), to be deployed by the mother spacecraft, that brings itself onto a collisional trajectory with the targeted planetary body in a simple manner. A possible science payload includes instruments for surface imaging, characterisation of the neutral exosphere, and magnetic field and plasma measurement near the target body down to very low-altitudes ( 1 km), during the probe's fast ( km/s) descent to the surface until impact. The science goals and the concept of operation are discussed with particular reference to Europa, including options for flying through water plumes and after-impact retrieval of very-low altitude science data. All in all, it is demonstrated how the descent probe has the potential to provide a high science return to a mission at a low extra level of complexity, engineering effort, and risk. This study builds upon earlier studies for a Callisto Descent Probe for the former Europa-Jupiter System Mission of ESA and NASA, and extends them with a detailed assessment of a descent probe designed to be an additional science payload for the NASA Europa Mission.

49 CFR 572.144 - Thorax assembly and test procedure.

Code of Federal Regulations, 2012 CFR

2012-10-01

... dummy (drawing 210-0000) is impacted by a test probe conforming to § 572.146(a) at 6.0 ±0.1 m/s (19.7 ±0... corridor, the peak force, measured by the probe-mounted accelerometer as defined in § 572.146(a) and... midsagittal plane so that the impact point of the longitudinal centerline of the probe coincides with the...

49 CFR 572.144 - Thorax assembly and test procedure.

Code of Federal Regulations, 2011 CFR

2011-10-01

... dummy (drawing 210-0000) is impacted by a test probe conforming to § 572.146(a) at 6.0 ±0.1 m/s (19.7 ±0... corridor, the peak force, measured by the probe-mounted accelerometer as defined in § 572.146(a) and... midsagittal plane so that the impact point of the longitudinal centerline of the probe coincides with the...

49 CFR 572.144 - Thorax assembly and test procedure.

Code of Federal Regulations, 2013 CFR

2013-10-01

... dummy (drawing 210-0000) is impacted by a test probe conforming to § 572.146(a) at 6.0 ±0.1 m/s (19.7 ±0... corridor, the peak force, measured by the probe-mounted accelerometer as defined in § 572.146(a) and... midsagittal plane so that the impact point of the longitudinal centerline of the probe coincides with the...

49 CFR 572.144 - Thorax assembly and test procedure.

Code of Federal Regulations, 2014 CFR

2014-10-01

... dummy (drawing 210-0000) is impacted by a test probe conforming to § 572.146(a) at 6.0 ±0.1 m/s (19.7 ±0... corridor, the peak force, measured by the probe-mounted accelerometer as defined in § 572.146(a) and... midsagittal plane so that the impact point of the longitudinal centerline of the probe coincides with the...

Kilometer-Scale Transient Atmospheres for Kinetic Payload Deposition on Icy Bodies

NASA Astrophysics Data System (ADS)

Koch, James

Entry, descent, and landing technologies for space exploration missions to atmospheric bodies traditionally exploit the body's ambient atmosphere as a medium through which a spacecraft or probe can interact to transfer momentum and energy for a soft landing. For bodies with no appreciable atmosphere, a significant engineering challenge exists to overcome the lack of passive methods to decelerate a spacecraft or probe. Proposed is a novel means for the creation of a transient atmosphere for airless icy bodies through the use of a two stage payload-penetrator probe. The first stage is a hyper-velocity penetrator that impacts the icy body. The second stage is an aero-braking-capable probe directed to pass through the ejecta plume from the hyper-velocity impact. Both experimental and computational studies show that a controlled high-energy impact can direct and transfer energy and momentum to a probe via a collimated ejecta plume. In an effort to provide clarity to this unexplored class of missions, a modeling-based engineering approach is taken to provide a first-order estimation of some of the involved physical phenomena. Three sub-studies are presented: an examination and characterization of ice plumes, modeling plume-probe interaction, and the extension of plume modeling as the basis for conceptual mission design. The modeling efforts are centered about two modeling formulations: smoothed particle hydrodynamics (SPH) and the arbitrary Largrangian-Eulerian (ALE) set of techniques. A database of fully-developed hypervelocity impacts and their associated plumes is created and used as inputs to a 1-D mathematical model for the interaction of a continuum-based plume and probe. A parametric study based on the hyper-velocity impact and staging of the probe-penetrator system is presented and discussed. Shown is that a tuned penetrator-probe mission has the potential to increase spacecraft payload mass fraction over conventional soft landing schemes.

49 CFR 572.186 - Abdomen assembly.

Code of Federal Regulations, 2014 CFR

2014-10-01

... longitudinal axis of the probe's impact face as shown in Figure U5-A in appendix A to this subpart; (4) The... the three load cell forces must be concurrent in time. (2) Maximum impactor force (impact probe...

Tests of the equivalence principle and gravitation theory using solar system bodies

NASA Technical Reports Server (NTRS)

Nordtvedt, K., Jr.

1971-01-01

The M sub g/M sub i ratio (ratio of body acceleration to gravitation field) of celestial bodies was measured. Deep probes of the post-Newtonian structure of gravitational theories are indicated. Kepler's third law is considered for the Sun-Jupiter system.

Deep Impact Spots Quarry

NASA Image and Video Library

2005-04-27

Taken on April 25, 2005, sixty-nine days before it gets up-close-and-personal with a comet, NASA Deep Impact spacecraft successfully photographed its quarry, comet Tempel 1, at a distance of 39.7 million miles.

KSC-05PD-0133

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. From the nearby Press Site at Cape Canaveral Air Force Station, Fla., photographers capture the exciting launch of the Deep Impact spacecraft at 1:47 p.m. EST. A NASA Discovery mission, Deep Impact is heading for space and a rendezvous 83 million miles from Earth with Comet Tempel 1. After releasing a 3- by 3-foot projectile (impactor) to crash onto the surface July 4, 2005, Deep Impacts flyby spacecraft will reveal the secrets of the comets interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network.

KSC-05PD-0134

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. Erupting from the flames and smoke beneath it, NASAs Deep Impact spacecraft lifts off at 1:47 p.m. EST today from Launch Pad 17-B, Cape Canaveral Air Force Station, Fla. A NASA Discovery mission, Deep Impact is heading for space and a rendezvous 83 million miles from Earth with Comet Tempel 1. After releasing a 3- by 3-foot projectile (impactor) to crash onto the surface July 4, 2005, Deep Impacts flyby spacecraft will reveal the secrets of the comets interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network.

KSC-05PD-0131

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. Erupting from the flames and smoke beneath it, NASAs Deep Impact spacecraft lifts off at 1:47 p.m. EST today from Launch Pad 17-B, Cape Canaveral Air Force Station, Fla. A NASA Discovery mission, Deep Impact is heading for space and a rendezvous 83 million miles from Earth with Comet Tempel 1. After releasing a 3- by 3-foot projectile (impactor) to crash onto the surface July 4, 2005, Deep Impacts flyby spacecraft will reveal the secrets of the comets interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network.

KSC-05PD-0135

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. Erupting from the flames and smoke beneath it, NASAs Deep Impact spacecraft lifts off at 1:47 p.m. EST today from Launch Pad 17-B, Cape Canaveral Air Force Station, Fla. A NASA Discovery mission, Deep Impact is heading for space and a rendezvous 83 million miles from Earth with Comet Tempel 1. After releasing a 3- by 3-foot projectile (impactor) to crash onto the surface July 4, 2005, Deep Impacts flyby spacecraft will reveal the secrets of the comets interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network.

KSC-05PD-0136

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. Engulfed by flames and smoke, NASAs Deep Impact spacecraft lifts off at 1:47 p.m. EST today from Launch Pad 17-B, Cape Canaveral Air Force Station, Fla. A NASA Discovery mission, Deep Impact is heading for space and a rendezvous 83 million miles from Earth with Comet Tempel 1. After releasing a 3- by 3-foot projectile (impactor) to crash onto the surface July 4, 2005, Deep Impacts flyby spacecraft will reveal the secrets of the comets interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network.

KSC-05PD-0130

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. With a burst of flames, NASAs Deep Impact spacecraft lifts off at 1:47 p.m. EST today from Launch Pad 17-B, Cape Canaveral Air Force Station, Fla. A NASA Discovery mission, Deep Impact is heading for space and a rendezvous 83 million miles from Earth with Comet Tempel 1. After releasing a 3- by 3-foot projectile (impactor) to crash onto the surface July 4, 2005, Deep Impacts flyby spacecraft will reveal the secrets of the comets interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network.

Coral communities as indicators of ecosystem-level impacts of the Deepwater Horizon spill

USGS Publications Warehouse

Fisher, Charles R.; Demopoulos, Amanda W.J.; Cordes, Erik E.; Baums, Iliana B.; White, Helen K.; Bourque, Jill R.

2014-01-01

The Macondo oil spill released massive quantities of oil and gas from a depth of 1500 meters. Although a buoyant plume carried released hydrocarbons to the sea surface, as much as half stayed in the water column and much of that in the deep sea. After the hydrocarbons reached the surface, weathering processes, burning, and the use of a dispersant caused hydrocarbon-rich marine snow to sink into the deep sea. As a result, this spill had a greater potential to affect deep-sea communities than had any previous spill. Here, we review the literature on impacts on deep-sea communities from the Macondo blowout and provide additional data on sediment hydrocarbon loads and the impacts on sediment infauna in areas with coral communities around the Macondo well. We review the literature on the genetic connectivity of deep-sea species in the Gulf of Mexico and discuss the potential for wider effects on deep Gulf coral communities.

Impact of Deepwater Horizon Spill on food supply to deep-sea benthos communities

USGS Publications Warehouse

Prouty, Nancy G.; Swarzenski, Pamela; Mienis, Furu; Duineveld, Gerald; Demopoulos, Amanda W.J.; Ross, Steve W.; Brooke, Sandra

2016-01-01

Deep-sea ecosystems encompass unique and often fragile communities that are sensitive to a variety of anthropogenic and natural impacts. After the 2010 Deepwater Horizon (DWH) oil spill, sampling efforts documented the acute impact of the spill on some deep-sea coral colonies. To investigate the impact of the DWH spill on quality and quantity of biomass delivered to the deep-sea, a suite of geochemical tracers (e.g., stable and radio-isotopes, lipid biomarkers, and compound specific isotopes) was measured from monthly sediment trap samples deployed near a high-density deep-coral site in the Viosca Knoll area of the north-central Gulf of Mexico prior to (Oct-2008 to Sept-2009) and after the spill (Oct-10 to Sept-11). Marine (e.g., autochthonous) sources of organic matter dominated the sediment traps in both years, however after the spill, there was a pronounced reduction in marinesourced OM, including a reduction in marine-sourced sterols and n-alkanes and a concomitant decrease in sediment trap organic carbon and pigment flux. Results from this study indicate a reduction in primary production and carbon export to the deep-sea in 2010-2011, at least 6-18 months after the spill started. Whereas satellite observations indicate an initial increase in phytoplankton biomass, results from this sediment trap study define a reduction in primary production and carbon export to the deep-sea community. In addition, a dilution from a low-14C carbon source (e.g., petrocarbon) was detected in the sediment trap samples after the spill, in conjunction with a change in the petrogenic composition. The data presented here fills a critical gap in our knowledge of biogeochemical processes and sub-acute impacts to the deep-sea that ensued after the 2010 DWH spill.

Deep Impact: 19 gigajoules can make quite an impression

NASA Technical Reports Server (NTRS)

Kubitschek, D.; Bank, T.; Frazier, W.; Blume, W.; Null, G.; Mastrodemos, N.; Synnott, S.

2001-01-01

Deep Impact will impact the comet Tempel-1 on July 4, 2005. The impact event will be clearly visible from small telescopes on Earth, especially in the IR bands. When combined with observations taken from the Flyby spacecraft, this science data set will provide unique insight into the materials and structure within the comet, and the strength of the surface.

The Impact of Chemical Probes in Drug Discovery: A Pharmaceutical Industry Perspective.

PubMed

Garbaccio, Robert M; Parmee, Emma R

2016-01-21

Chemical probes represent an important component of both academic and pharmaceutical drug discovery research. As a complement to prior reviews that have defined this scientific field, we aim to provide an industry perspective on the value of having high-quality chemical probes throughout the course of preclinical research. By studying examples from the internal Merck pipeline, we recognize that these probes require significant collaborative investment to realize their potential impact in clarifying the tractability and translation of a given therapeutic target. This perspective concludes with recommendations for chemical probe discovery aimed toward maximizing their potential to identify targets that result in the successful delivery of novel therapeutics. Copyright © 2016 Elsevier Ltd. All rights reserved.

On the "Voter Awareness" in Kafka's Novels

ERIC Educational Resources Information Center

Wang, Jing

2009-01-01

"The Bible" stressed the Jews are God's voters. The "voter awareness" infiltrated into the souls of all Jews. Kafka was a Jewish writer. The national consciousness from the deep soul affected his creations. This paper probes into the voter awareness interpreted in Kafka's novels from three aspects: voters wandering at alien…

Modeling the Deep Impact Near-nucleus Observations of H2O and CO2 in Comet 9P/Tempel 1 Using Asymmetric Spherical Coupled Escape Probability

NASA Astrophysics Data System (ADS)

Gersch, Alan M.; A’Hearn, Michael F.; Feaga, Lori M.

2018-04-01

We have applied our asymmetric spherical adaptation of Coupled Escape Probability to the modeling of optically thick cometary comae. Expanding on our previously published work, here we present models including asymmetric comae. Near-nucleus observations from the Deep Impact mission have been modeled, including observed coma morphology features. We present results for two primary volatile species of interest, H2O and CO2, for comet 9P/Tempel 1. Production rates calculated using our best-fit models are notably greater than those derived from the Deep Impact data based on the assumption of optically thin conditions, both for H2O and CO2 but more so for CO2, and fall between the Deep Impact values and the global pre-impact production rates measured at other observatories and published by Schleicher et al. (2006), Mumma et al. (2005), and Mäkinen et al. (2007).

Man and the Last Great Wilderness: Human Impact on the Deep Sea

PubMed Central

Ramirez-Llodra, Eva; Tyler, Paul A.; Baker, Maria C.; Bergstad, Odd Aksel; Clark, Malcolm R.; Escobar, Elva; Levin, Lisa A.; Menot, Lenaick; Rowden, Ashley A.; Smith, Craig R.; Van Dover, Cindy L.

2011-01-01

The deep sea, the largest ecosystem on Earth and one of the least studied, harbours high biodiversity and provides a wealth of resources. Although humans have used the oceans for millennia, technological developments now allow exploitation of fisheries resources, hydrocarbons and minerals below 2000 m depth. The remoteness of the deep seafloor has promoted the disposal of residues and litter. Ocean acidification and climate change now bring a new dimension of global effects. Thus the challenges facing the deep sea are large and accelerating, providing a new imperative for the science community, industry and national and international organizations to work together to develop successful exploitation management and conservation of the deep-sea ecosystem. This paper provides scientific expert judgement and a semi-quantitative analysis of past, present and future impacts of human-related activities on global deep-sea habitats within three categories: disposal, exploitation and climate change. The analysis is the result of a Census of Marine Life – SYNDEEP workshop (September 2008). A detailed review of known impacts and their effects is provided. The analysis shows how, in recent decades, the most significant anthropogenic activities that affect the deep sea have evolved from mainly disposal (past) to exploitation (present). We predict that from now and into the future, increases in atmospheric CO2 and facets and consequences of climate change will have the most impact on deep-sea habitats and their fauna. Synergies between different anthropogenic pressures and associated effects are discussed, indicating that most synergies are related to increased atmospheric CO2 and climate change effects. We identify deep-sea ecosystems we believe are at higher risk from human impacts in the near future: benthic communities on sedimentary upper slopes, cold-water corals, canyon benthic communities and seamount pelagic and benthic communities. We finalise this review with a short discussion on protection and management methods. PMID:21829635

Photoacoustic imaging of fluorophores using pump-probe excitation

PubMed Central

Märk, Julia; Schmitt, Franz-Josef; Theiss, Christoph; Dortay, Hakan; Friedrich, Thomas; Laufer, Jan

2015-01-01

A pump-probe technique for the detection of fluorophores in tomographic PA images is introduced. It is based on inducing stimulated emission in fluorescent molecules, which in turn modulates the amount of thermalized energy, and hence the PA signal amplitude. A theoretical model of the PA signal generation in fluorophores is presented and experimentally validated on cuvette measurements made in solutions of Rhodamine 6G, a fluorophore of known optical and molecular properties. The application of this technique to deep tissue tomographic PA imaging is demonstrated by determining the spatial distribution of a near-infrared fluorophore in a tissue phantom. PMID:26203378

Late Eocene impact events recorded in deep-sea sediments

NASA Technical Reports Server (NTRS)

Glass, B. P.

1988-01-01

Raup and Sepkoski proposed that mass extinctions have occurred every 26 Myr during the last 250 Myr. In order to explain this 26 Myr periodicity, it was proposed that the mass extinctions were caused by periodic increases in cometary impacts. One method to test this hypothesis is to determine if there were periodic increases in impact events (based on crater ages) that correlate with mass extinctions. A way to test the hypothesis that mass extinctions were caused by periodic increases in impact cratering is to look for evidence of impact events in deep-sea deposits. This method allows direct observation of the temporal relationship between impact events and extinctions as recorded in the sedimentary record. There is evidence in the deep-sea record for two (possibly three) impact events in the late Eocene. The younger event, represented by the North American microtektite layer, is not associated with an Ir anomaly. The older event, defined by the cpx spherule layer, is associated with an Ir anomaly. However, neither of the two impact events recorded in late Eocene deposits appears to be associated with an unusual number of extinctions. Thus there is little evidence in the deep-sea record for an impact-related mass extinction in the late Eocene.

Microbial abundance in the deep subsurface of the Chesapeake Bay impact crater: Relationship to lithology and impact processes

USGS Publications Warehouse

Cockell, Charles S.; Gronstal, Aaron L.; Voytek, Mary A.; Kirshtein, Julie D.; Finster, Kai; Sanford, Ward E.; Glamoclija, Mihaela; Gohn, Gregroy S.; Powars, David S.; Horton, J. Wright

2009-01-01

Asteroid and comet impact events are known to cause profound disruption to surface ecosystems. The aseptic collection of samples throughout a 1.76-km-deep set of cores recovered from the deep subsurface of the Chesapeake Bay impact structure has allowed the study of the subsurface biosphere in a region disrupted by an impactor. Microbiological enumerations suggest the presence of three major microbiological zones. The upper zone (127–867 m) is characterized by a logarithmic decline in microbial abundance from the surface through the postimpact section of Miocene to Upper Eocene marine sediments and across the transition into the upper layers of the impact tsunami resurge sediments and sediment megablocks. In the middle zone (867–1397 m) microbial abundances are below detection. This zone is predominantly quartz sand, primarily composed of boulders and blocks, and it may have been mostly sterilized by the thermal pulse delivered during impact. No samples were collected from the large granite block (1096–1371 m). The lowest zone (below 1397 m) of increasing microbial abundance coincides with a region of heavily impact-fractured, hydraulically conductive suevite and fractured schist. These zones correspond to lithologies influenced by impact processes. Our results yield insights into the influence of impacts on the deep subsurface biosphere.

Deep-Draft Navigation User Charges : Recovery Options and Impacts

DOT National Transportation Integrated Search

1977-08-01

Alternative cost recovery options for Federal deep-draft navigation expenditures are investigated and the impacts of user charges on waterborne trades and commodity traffic, both foreign and domestic (Great Lakes and coastwise), are assessed. In addi...

Deep Impact on Its Way

NASA Image and Video Library

2005-01-18

The high speed of NASA Deep Impact spacecraft causes it to appear as a long streak across the sky in the constellation Virgo during the 10-minute exposure time of this photograph taken by Mr. Palomar 200-inch telescope.

KSC-05PP-0138

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. Emerging through the smoke and steam, the Boeing Delta II rocket carrying NASAs Deep Impact spacecraft lifts off at 1:47 p.m. EST from Launch Pad 17-B, Cape Canaveral Air Force Station, Fla. A NASA Discovery mission, Deep Impact is heading for space and a rendezvous 83 million miles from Earth with Comet Tempel 1. After releasing a 3- by 3-foot projectile (impactor) to crash onto the surface July 4, 2005, Deep Impacts flyby spacecraft will reveal the secrets of the comets interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network.

KSC-05PD-0137

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. After a perfect liftoff at 1:47 p.m. EST today from Launch Pad 17-B, Cape Canaveral Air Force Station, Fla., the Boeing Delta II rocket with Deep Impact spacecraft aboard soars through the clear blue sky. A NASA Discovery mission, Deep Impact is heading for space and a rendezvous 83 million miles from Earth with Comet Tempel 1. After releasing a 3- by 3-foot projectile (impactor) to crash onto the surface July 4, 2005, Deep Impacts flyby spacecraft will reveal the secrets of the comets interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network.

KSC-05PD-0132

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. Guests of NASA gather near the launch site at Cape Canaveral Air Force Station, Fla., to watch the Deep Impact spacecraft as it speeds through the air after a perfect launch at 1:47 p.m. EST. A NASA Discovery mission, Deep Impact is heading for space and a rendezvous 83 million miles from Earth with Comet Tempel 1. After releasing a 3- by 3-foot projectile (impactor) to crash onto the surface July 4, 2005, Deep Impacts flyby spacecraft will reveal the secrets of the comets interior by collecting pictures and data of how the crater forms, measuring the craters depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network.

Proposed measurement of tagged deep inelastic scattering in Hall A of Jefferson lab

DOE Office of Scientific and Technical Information (OSTI.GOV)

Montgomery, Rachel; Annand, John; Dutta, Dipangkar

2017-03-01

A tagged deep inelastic scattering (TDIS) experiment is planned for Hall A of Jefferson Lab, which will probe the mesonic content of the nucleon directly. Low momentum recoiling (and spectator) protons will be measured in coincidence with electrons scattered in a deep inelastic regime from hydrogen (and deuterium) targets, covering kinematics of 8 < W2 < 18 GeV2, 1 < Q2 < 3 (GeV/c)2 and 0:05 < x < 0:2. The tagging technique will help identify scattering from partons in the meson cloud and provide access to the pion structure function via the Sullivan process. The experiment will yield themore » first TDIS results in the valence regime, for both proton and neutron targets. We present here an overview of the experiment.« less

The Chandra Deep Wide-Field Survey: Completing the new generation of Chandra extragalactic surveys

NASA Astrophysics Data System (ADS)

Hickox, Ryan

2016-09-01

Chandra X-ray surveys have revolutionized our view of the growth of black holes across cosmic time. Recently, fundamental questions have emerged about the connection of AGN to their host large scale structures that clearly demand a wide, deep survey over a large area, comparable to the recent extensive Chandra surveys in smaller fields. We propose the Chandra Deep Wide-Field Survey (CDWFS) covering the central 6 sq. deg in the Bootes field, totaling 1.025 Ms (building on 550 ks from the HRC GTO program). CDWFS will efficiently probe a large cosmic volume, allowing us to carry out accurate new investigations of the connections between black holes and their large-scale structures, and will complete the next generation surveys that comprise a key part of Chandra's legacy.

NASA's next generation all-digital deep space network breadboard receiver

NASA Technical Reports Server (NTRS)

Hinedi, Sami

1993-01-01

This paper describes the breadboard advanced receiver (ARX) that is currently being built for future use in NASA's deep space network (DSN). This receiver has unique requirements in having to operate with very weak signals from deep space probes and provide high quality telemetry and tracking data. The hybrid analog/digital receiver performs multiple functions including carrier, subcarrier and symbol synchronization. Tracking can be achieved for either residual, suppressed or hybrid carriers and for both sinusoidal and square wave subcarriers. System requirements are specified and a functional description of the ARX is presented. The various digital signal processing algorithms used are also discussed and illustrated with block diagrams. Other functions such as time tagged Doppler extraction and monitor/control are also discussed including acquisition algorithms and lock detection schemes.

Semi-inclusive Deep Inelastic Scattering at Small-x

DOE Office of Scientific and Technical Information (OSTI.GOV)

Marquet, C.; Xiao, B.-W.; Yuan, Feng

We study the semi-inclusive hadron production in deep inelastic scattering at small-x.A transverse momentum dependent factorization is found consistent with the resultscalculated in the color-dipole framework in the appropriate kinematic region. The transverse momentum dependent quark distribution can be studied in this processas a probe for the small-x saturation physics. Especially, the ratio of the quark distributions as functions of transverse momentum at different x demonstrates strong dependence on the saturation scale. The Q2 dependence of the same ratio is also studied by applying the Collins-Soper-Sterman resummation method.

The Boundary of Tectonic Units of the South China Continent in the Meso-Neoproterozoic - Early Paleozoic: Insights from Integrated Geophysical Study

NASA Astrophysics Data System (ADS)

Guo, L.; Gao, R.; Meng, X.; Zhang, J.; Wang, H.; Liu, Y.

2013-12-01

The South China continent (SCC), located in the transition zone of the Eurasia, India and Pacific plates, formed in the Meso-Neoproterozoic by collision of the Yangtze block and the Cathaysia block. However, the boundaries of the two blocks before the late Paleozoic (from Meso-Neoproterozoic to early Paleozoic) remain debated in the literature due to strong and complex tectonic and magmatic activities since then. The south of Jiangnan archicontinent is covered mostly by the thick strata since the late Paleozoic, the surface of which is widely covered by the vegetation. And the regional tectonic deformation is extremely complicated with few basal outcrops. For decades, a variety of geophysical detections have been performed in the SCC for understanding the deep structure and tectonic evolution, including deep seismic sounding (DSS) profiles, magnetotelluric sounding (MT) profiles, gravity and magnetic surveys and a small amount of deep seismic reflection profiles. However, due to the limitations of resolution and accuracy of the observed geophysical data in the past, especially short of the deep seismic reflection profiles to reveal fine lithosphere structure, different scientists presented various views on the division of tectonic units in the SCC. In quite recent years, the SinoProbe-02 project launched a long profile of geophysical detections across the two blocks in the SCC, including deep seismic reflection, DSS, MT, and broadband seismic observation, the resolution and accuracy of which had been improved greatly. These newly data will benefit better understanding the deep structure and tectonic evolution of the SCC. Here, we assembled high-resolution Bouguer gravity anomalies and aeromagnetic anomalies data in the SCC. The magnetic data were reduced to the pole by used a varying magnetic inclinations algorithm. We then performed anomaly separation and multi-scales lineation structure analysis on the gravity and RTP magnetic data, and then did 3D fusion analysis on them. Seismic reflection profiles focus on fine lithosphere structure vertically along the profile, while gravity and magnetic methods are beneficial to reveal regional tectonic features laterally. The integrate study of seismic, gravity and magnetic data will play the advantages of various methods and constraint and confirm each other. Hence, we did the interpretation of gravity and magnetic data with constraints of the newly seismic reflection profile. Based on the above studies, we traced the boundaries of tectonic units in the SCC from Meso-Neoproterozoic to early Paleozoic, and formed a certain understanding of the tectonic evolution in the SCC before the late Paleozoic. Acknowledgment: We acknowledge the financial support of the SinoProbe-02-01 and SinoProbe-01-05 projects, and the Fundamental Research Funds for the Central Universities.

Human Activities on the Deep Seafloor in the North East Atlantic: An Assessment of Spatial Extent

PubMed Central

Benn, Angela R.; Weaver, Philip P.; Billet, David S. M.; van den Hove, Sybille; Murdock, Andrew P.; Doneghan, Gemma B.; Le Bas, Tim

2010-01-01

Background Environmental impacts of human activities on the deep seafloor are of increasing concern. While activities within waters shallower than 200 m have been the focus of previous assessments of anthropogenic impacts, no study has quantified the extent of individual activities or determined the relative severity of each type of impact in the deep sea. Methodology The OSPAR maritime area of the North East Atlantic was chosen for the study because it is considered to be one of the most heavily impacted by human activities. In addition, it was assumed data would be accessible and comprehensive. Using the available data we map and estimate the spatial extent of five major human activities in the North East Atlantic that impact the deep seafloor: submarine communication cables, marine scientific research, oil and gas industry, bottom trawling and the historical dumping of radioactive waste, munitions and chemical weapons. It was not possible to map military activities. The extent of each activity has been quantified for a single year, 2005. Principal Findings Human activities on the deep seafloor of the OSPAR area of the North Atlantic are significant but their footprints vary. Some activities have an immediate impact after which seafloor communities could re-establish, while others can continue to make an impact for many years and the impact could extend far beyond the physical disturbance. The spatial extent of waste disposal, telecommunication cables, the hydrocarbon industry and marine research activities is relatively small. The extent of bottom trawling is very significant and, even on the lowest possible estimates, is an order of magnitude greater than the total extent of all the other activities. Conclusions/Significance To meet future ecosystem-based management and governance objectives for the deep sea significant improvements are required in data collection and availability as well as a greater awareness of the relative impact of each human activity. PMID:20856885

Human activities on the deep seafloor in the North East Atlantic: an assessment of spatial extent.

PubMed

Benn, Angela R; Weaver, Philip P; Billet, David S M; van den Hove, Sybille; Murdock, Andrew P; Doneghan, Gemma B; Le Bas, Tim

2010-09-13

Environmental impacts of human activities on the deep seafloor are of increasing concern. While activities within waters shallower than 200 m have been the focus of previous assessments of anthropogenic impacts, no study has quantified the extent of individual activities or determined the relative severity of each type of impact in the deep sea. The OSPAR maritime area of the North East Atlantic was chosen for the study because it is considered to be one of the most heavily impacted by human activities. In addition, it was assumed data would be accessible and comprehensive. Using the available data we map and estimate the spatial extent of five major human activities in the North East Atlantic that impact the deep seafloor: submarine communication cables, marine scientific research, oil and gas industry, bottom trawling and the historical dumping of radioactive waste, munitions and chemical weapons. It was not possible to map military activities. The extent of each activity has been quantified for a single year, 2005. Human activities on the deep seafloor of the OSPAR area of the North Atlantic are significant but their footprints vary. Some activities have an immediate impact after which seafloor communities could re-establish, while others can continue to make an impact for many years and the impact could extend far beyond the physical disturbance. The spatial extent of waste disposal, telecommunication cables, the hydrocarbon industry and marine research activities is relatively small. The extent of bottom trawling is very significant and, even on the lowest possible estimates, is an order of magnitude greater than the total extent of all the other activities. To meet future ecosystem-based management and governance objectives for the deep sea significant improvements are required in data collection and availability as well as a greater awareness of the relative impact of each human activity.

Design and Testing of a Flexible Inclinometer Probe for Model Tests of Landslide Deep Displacement Measurement.

PubMed

Zhang, Yongquan; Tang, Huiming; Li, Changdong; Lu, Guiying; Cai, Yi; Zhang, Junrong; Tan, Fulin

2018-01-14

The physical model test of landslides is important for studying landslide structural damage, and parameter measurement is key in this process. To meet the measurement requirements for deep displacement in landslide physical models, an automatic flexible inclinometer probe with good coupling and large deformation capacity was designed. The flexible inclinometer probe consists of several gravity acceleration sensing units that are protected and positioned by silicon encapsulation, all the units are connected to a 485-comunication bus. By sensing the two-axis tilt angle, the direction and magnitude of the displacement for a measurement unit can be calculated, then the overall displacement is accumulated according to all units, integrated from bottom to top in turn. In the conversion from angle to displacement, two spline interpolation methods are introduced to correct and resample the data; one is to interpolate the displacement after conversion, and the other is to interpolate the angle before conversion; compared with the result read from checkered paper, the latter is proved to have a better effect, with an additional condition that the displacement curve move up half the length of the unit. The flexible inclinometer is verified with respect to its principle and arrangement by a laboratory physical model test, and the test results are highly consistent with the actual deformation of the landslide model.

Optical coherence tomography and optical coherence domain reflectometry for deep brain stimulation probe guidance

NASA Astrophysics Data System (ADS)

Jeon, Sung W.; Shure, Mark A.; Baker, Kenneth B.; Chahlavi, Ali; Hatoum, Nagi; Turbay, Massud; Rollins, Andrew M.; Rezai, Ali R.; Huang, David

2005-04-01

Deep Brain Stimulation (DBS) is FDA-approved for the treatment of Parkinson's disease and essential tremor. Currently, placement of DBS leads is guided through a combination of anatomical targeting and intraoperative microelectrode recordings. The physiological mapping process requires several hours, and each pass of the microelectrode into the brain increases the risk of hemorrhage. Optical Coherence Domain Reflectometry (OCDR) in combination with current methodologies could reduce surgical time and increase accuracy and safety by providing data on structures some distance ahead of the probe. For this preliminary study, we scanned a rat brain in vitro using polarization-insensitive Optical Coherence Tomography (OCT). For accurate measurement of intensity and attenuation, polarization effects arising from tissue birefringence are removed by polarization diversity detection. A fresh rat brain was sectioned along the coronal plane and immersed in a 5 mm cuvette with saline solution. OCT images from a 1294 nm light source showed depth profiles up to 2 mm. Light intensity and attenuation rate distinguished various tissue structures such as hippocampus, cortex, external capsule, internal capsule, and optic tract. Attenuation coefficient is determined by linear fitting of the single scattering regime in averaged A-scans where Beer"s law is applicable. Histology showed very good correlation with OCT images. From the preliminary study using OCT, we conclude that OCDR is a promising approach for guiding DBS probe placement.

Probing the outer limits of a galactic halo - deep imaging of exceptionally remote globular clusters in M31

NASA Astrophysics Data System (ADS)

Mackey, Dougal

2011-10-01

Globular clusters {GCs} are fossil relics from which we can obtain critical insights into the formation and growth of galaxies. As part of the ongoing Pan-Andromeda Archaeological Survey {PAndAS} we have discovered a group of exceptionally remote GCs in the M31 halo, spanning a range in projected galactocentric distance of 85-145 kpc. Here we apply for deep ACS imaging of 13 such targets, which will allow us to study their constituent stellar populations, line-of-sight distances, and structural parameters. Our measurements will facilitate the use of these GCs as a unique set of probes of the exceptionally remote halo of a large disk galaxy, opening up a completely new area of parameter space to observational constraint. Comparing the properties of our targets with more centrally-located objects will provide a much clearer picture of the M31 GC population than is presently available, while comparison with the outermost Milky Way GCs will further elucidate well-known disparities between the two systems and offer vital clues to differences in their assembly. In addition, our measurements will substantially augment a broad swathe of science that is presently underway - including probing the dark mass distribution in M31 at very large radii, and investigating the detailed chemical composition of M31 GCs via high-resolution integrated-light spectroscopy.

Design and Testing of a Flexible Inclinometer Probe for Model Tests of Landslide Deep Displacement Measurement

PubMed Central

Zhang, Yongquan; Tang, Huiming; Li, Changdong; Lu, Guiying; Cai, Yi; Zhang, Junrong; Tan, Fulin

2018-01-01

The physical model test of landslides is important for studying landslide structural damage, and parameter measurement is key in this process. To meet the measurement requirements for deep displacement in landslide physical models, an automatic flexible inclinometer probe with good coupling and large deformation capacity was designed. The flexible inclinometer probe consists of several gravity acceleration sensing units that are protected and positioned by silicon encapsulation, all the units are connected to a 485-comunication bus. By sensing the two-axis tilt angle, the direction and magnitude of the displacement for a measurement unit can be calculated, then the overall displacement is accumulated according to all units, integrated from bottom to top in turn. In the conversion from angle to displacement, two spline interpolation methods are introduced to correct and resample the data; one is to interpolate the displacement after conversion, and the other is to interpolate the angle before conversion; compared with the result read from checkered paper, the latter is proved to have a better effect, with an additional condition that the displacement curve move up half the length of the unit. The flexible inclinometer is verified with respect to its principle and arrangement by a laboratory physical model test, and the test results are highly consistent with the actual deformation of the landslide model. PMID:29342902

Visualization of Space-Time Ambiguities to be Explored by the NASA GEC Mission with a Critique of Synthesized Measurements for Different GEC Mission Scenarios

NASA Technical Reports Server (NTRS)

Sojka, Jan J.; Zhu, Lie; Fuller-Rowell, Timothy J.

2005-01-01

The objective of this grant was to study how a multi-satellite mission configuration can be optimized for maximum exploratory scientific return. NASA's Solar Terrestrial Probe (STP) concept mission Geospace Electrodynamic Connections (GEC) was the target mission for this pilot study. GEC prime mission characteristics were two fold: (i) a series of three satellites in the same orbit plane with differential spacing, and (ii) a deep-dipping phase in which these satellites could dip to altitudes as low as 130 km to explore the lower ionosphere and thermosphere. Each satellite would carry a full suite of plasma and neutral in-situ sensors and have the same dipping capability. This latter aspect would be envisaged as a series, up to 10, of deep-dipping campaigns, each lasting 10 days during which the perigee would be lowered to the desired probing depth. The challenge in optimization is to establish the scientific problems that can best be addressed by varying or selecting satellite spacing during a two-year mission while also interspersing, in this two year time frame, the deep-dipping campaigns. Although this sounds like a straightforward trade-off situation, it is complicated by the orbit precession in local time, the location of perigee, and that even the dipping campaigns will have preferred satellite spacing requirements.

Ice Particle Impact on Cloud Water Content Instrumentation

NASA Technical Reports Server (NTRS)

Emery, Edward F.; Miller, Dean R.; Plaskon, Stephen R.; Strapp, Walter; Lillie, Lyle

2004-01-01

Determining the total amount of water contained in an icing cloud necessitates the measurement of both the liquid droplets and ice particles. One commonly accepted method for measuring cloud water content utilizes a hot wire sensing element, which is maintained at a constant temperature. In this approach, the cloud water content is equated with the power required to keep the sense element at a constant temperature. This method inherently assumes that impinging cloud particles remain on the sensing element surface long enough to be evaporated. In the case of ice particles, this assumption requires that the particles do not bounce off the surface after impact. Recent tests aimed at characterizing ice particle impact on a thermally heated wing section, have raised questions about the validity of this assumption. Ice particles were observed to bounce off the heated wing section a very high percentage of the time. This result could have implications for Total Water Content sensors which are designed to capture ice particles, and thus do not account for bouncing or breakup of ice particles. Based on these results, a test was conducted to investigate ice particle impact on the sensing elements of the following hot-wire cloud water content probes: (1) Nevzorov Total Water Content (TWC)/Liquid Water Content (LWC) probe, (2) Science Engineering Associates TWC probe, and (3) Particle Measuring Systems King probe. Close-up video imaging was used to study ice particle impact on the sensing element of each probe. The measured water content from each probe was also determined for each cloud condition. This paper will present results from this investigation and attempt to evaluate the significance of ice particle impact on hot-wire cloud water content measurements.

Impact of science objectives and requirements on probe mission and system design

NASA Technical Reports Server (NTRS)

Ledbetter, K. W.

1974-01-01

Problem areas in probe science technology are discussed that require a solution before probe systems can actually be designed. Considered are the effects of the model atmospheres on probe design; secondly, the effects of implementing the requirements to locate and measure the clouds and, trade-offs between descent sampling and measurement criteria as they affect probe system design.

Body Temperature Measurements for Metabolic Phenotyping in Mice.

PubMed

Meyer, Carola W; Ootsuka, Youichirou; Romanovsky, Andrej A

2017-01-01

Key Points Rectal probing is subject to procedural bias. This method is suitable for first-line phenotyping, provided probe depth and measurement duration are standardized. It is also useful for detecting individuals with out-of-range body temperatures (during hypothermia, torpor).The colonic temperature attained by inserting the probe >2 cm deep is a measure of deep (core) body temperature.IR imaging of the skin is useful for detecting heat leaks and autonomous thermoregulatory alterations, but it does not measure body temperature.Temperature of the hairy or shaved skin covering the inter-scapular brown adipose tissue can be used as a measure of BAT thermogenesis. However, obtaining such measurements of sufficient quality is very difficult, and interpreting them can be tricky. Temperature differences between the inter-scapular and lumbar areas can be a better measure of the thermogenic activity of inter-scapular brown adipose tissue.Implanted probes for precise determination of BAT temperature (changes) should be fixed close to the Sulzer's vein. For measurement of BAT thermogenesis, core body temperature and BAT temperature should be recorded simultaneously.Tail temperature is suitable to compare the presence or absence of vasoconstriction or vasodilation.Continuous, longitudinal monitoring of core body temperature is preferred over single probing, as the readings are taken in a non-invasive, physiological context.Combining core body temperature measurements with metabolic rate measurements yields insights into the interplay between heat production and heat loss (thermal conductance), potentially revealing novel thermoregulatory phenotypes. Endothermic organisms rely on tightly balanced energy budgets to maintain a regulated body temperature and body mass. Metabolic phenotyping of mice, therefore, often includes the recording of body temperature. Thermometry in mice is conducted at various sites, using various devices and measurement practices, ranging from single-time probing to continuous temperature imaging. Whilst there is broad agreement that body temperature data is of value, procedural considerations of body temperature measurements in the context of metabolic phenotyping are missing. Here, we provide an overview of the various methods currently available for gathering body temperature data from mice. We explore the scope and limitations of thermometry in mice, with the hope of assisting researchers in the selection of appropriate approaches, and conditions, for comprehensive mouse phenotypic analyses.

Radiometric Thermometry for Wearable Deep Tissue Monitoring

NASA Astrophysics Data System (ADS)

Momenroodaki, Parisa

Microwave thermometry is an attractive non-invasive method for measuring internal body temperature. This approach has the potential of enabling a wearable device that can continuously monitor core body temperature. There are a number of health-related applications in both diagnostics and therapy, which can benefit from the knowledge of core body temperature. However,there are a limited number of device solutions, which are usually not wearable or cannot continuously monitor internal body temperature non-invasively. In this thesis, a possible path toward implementing such a thermometer is presented. The device operates in the "quiet" frequency band of 1.4 GHz which is chosen as a compromise between sensing depth and radio frequency interference (RFI). A major challenge in microwave thermometry is detecting small temperature variations of deep tissue layers from surface (skin) measurements. The type and thickness of tissue materials significantly affect the design of the probe, which has the function of receiving black-body radiation from tissues beneath it and coupling the power to a sensitive radiometric receiver. High dielectric constant contrast between skin, fat (/bone), and muscle layers suggests structures with dominant tangential component of the electric field, such as a patch or slot. Adding a layer of low-loss,low-dielectric constant superstrate can further reduce the contribution of superficial tissue layers in the received thermal noise. Several probe types are designed using a full-wave electromagnetic simulator, with a goal of maximizing the power reception from deep tissue layers. The designs are validated with a second software tool and various measurements. A stable, narrow-band, and highly sensitive radiometer is developed, enabling the device to operate in a non-shielded RF environment.To use the microwave thermometer in a RF congested environment, not only narrow-band probe and radiometers are used but an additional probe is introduced for observing the environmental interference. By applying an adaptive filter, the effect of RFI is mitigated in long-term measurements. Several solid and liquid tissue phantoms, required for accurate modeling of the probe and human body interaction, are also developed. The concept of human body microwave thermometry is validated through several measurements on the single-layer and multiple-layer tissue phantoms as well as on the surface of the human body, specifically on the cheek where the internal temperature can easily be changed and independently measured with a thermocouple. Measurement results prove the capability of the device in tracking the temperature of buried tissue layer phantoms to within 0.2K, as well as monitoring internal human body temperature.

Attitude Determination and Control Subsystem (ADCS) Preparations for the EPOXI Flyby of Comet Hartley 2

NASA Technical Reports Server (NTRS)

Luna, Michael E.; Collins, Steven M.

2011-01-01

On November 4, 2010 the former "Deep Impact" spacecraft, renamed "EPOXI" for its extended mission, flew within 700km of comet 103P/Hartley 2. In July 2005, the spacecraft had previously imaged a probe impact of comet Tempel 1. The EPOXI flyby was the fifth close encounter of a spacecraft with a comet nucleus and marked the first time in history that two comet nuclei were imaged at close range with the same suite of onboard science instruments. This challenging objective made the function of the attitude determination and control subsystem (ADCS) critical to the successful execution of the EPOXI flyby.As part of the spacecraft flyby preparations, the ADCS operations team had to perform meticulous sequence reviews, implement complex spacecraft engineering and science activities and perform numerous onboard calibrations. ADCS contributions included design and execution of 10 trajectory correction maneuvers, the science calibration of the two telescopic instruments, an in-flight demonstration of high-rate turns between Earth and comet point, and an ongoing assessment of reaction wheel health. The ADCS team was also responsible for command sequences that included updates to the onboard ephemeris and sun sensor coefficients and implementation of reaction wheel assembly (RWA) de-saturations.

Attitude Determination and Control Subsystem (ADCS) Preparations for the EPOXI Flyby of Comet Haley 2

NASA Technical Reports Server (NTRS)

Luna, Michael E.; Collins, Stephen M.

2011-01-01

On November 4, 2010 the already "in-flight" Deep Impact spacecraft flew within 700km of comet 103P/Hartley 2 as part of its extended mission EPOXI, the 5th time to date any spacecraft visited a comet. In 2005, the spacecraft had previously imaged a probe impact comet Tempel 1. The EPOXI flyby marked the first time in history that two comets were explored with the same instruments on a re-used spacecraft-with hardware and software originally designed and optimized for a different mission. This made the function of the attitude determination and control subsystem (ADCS) critical to the successful execution of the EPOXI flyby. As part of the spacecraft team preparations, the ADCS team had to perform thorough sequence reviews, key spacecraft activities and onboard calibrations. These activities included: review of background sequences for the initial conditions vector, sun sensor coefficients, and reaction wheel assembly (RWA) de-saturations; design and execution of 10 trajectory correction maneuvers; science calibration of the two telescope instruments; a flight demonstration of the fastest turns conducted by the spacecraft between Earth and comet point; and assessment of RWA health (given RWA problems on other spacecraft).

Nanodosimetry of Low Energy (0.1 - 100 eV) Cation Damage to DNA

NASA Astrophysics Data System (ADS)

Sellami, L.; Martin, F.; Hunting, D.; Lacombe, S.; Huels, M. A.

2004-03-01

The importance of heavy ions in radiobiology is twofold: (1) they represent the most efficient and volume selective mode of radiotherapy of deep-seated and non-operable tumors, (2) in space environments, or at supersonic altitudes, the most lethal radiation consists of cosmic rays which have a high efficiency to induce clustered DNA lesions, mutations, and cancer. Thus, the study of their effects on DNA is essential for radiation risk assessment, dosimetry, and efficient use of hadrontherapy. Here, we investigate damage to DNA and its components, induced by heavy ion impact, via a novel ion-plasma method, which allows us to probe ion energy depositions in the 0.1-100 eV/nm range in nanoscopic biomolecular films. Cations are generated by electron impact in ultra pure gases (Ar, N2, CO, etc.), and are uniformly accelerated by grids towards the inside surface of a cylinder where an organic film was deposited. After ion irradiation at a specific energy and ion dose, the film is recovered and analyzed. For DNA, gel electrophoresis is used to quantify yields of single, double, and multiple strand breaks. For DNA components (mononucleotides), fragmentation and new products are measured by HPLC and MS.

Relativistic electron dropout echoes induced by interplanetary shocks

NASA Astrophysics Data System (ADS)

Schiller, Q.; Kanekal, S. G.; Boyd, A. J.; Baker, D. N.; Blake, J. B.; Spence, H. E.

2017-12-01

Interplanetary shocks that impact Earth's magnetosphere can produce immediate and dramatic responses in the trapped relativistic electron population. One well-studied response is a prompt injection capable of transporting relativistic electrons deep into the magnetosphere and accelerating them to multi-MeV energies. The converse effect, electron dropout echoes, are observations of a sudden dropout of electron fluxes observed after the interplanetary shock arrival. Like the injection echo signatures, dropout echoes can also show clear energy dispersion signals. They are of particular interest because they have only recently been observed and their causal mechanism is not well understood. In the analysis presented here, we show observations of electron drift echo signatures from the Relativistic Electron-Proton Telescope (REPT) and Magnetic Electron and Ion Sensors (MagEIS) onboard NASA's Van Allen Probes mission, which show simultaneous prompt enhancements and dropouts within minutes of the associated with shock impact. We show that the observations associated with both enhancements and dropouts are explained by the inward motion caused by the electric field impulse induced by the interplanetary shock, and either energization to cause the enhancement, or lack of a seed population to cause the dropout.

Probing Metabolic Activity of Deep Subseafloor Life with NanoSIMS

NASA Astrophysics Data System (ADS)

Morono, Y.; Terada, T.; Itoh, M.; Inagaki, F.

2014-12-01

There are very few natural environments where life is absent in the Earth's surface biosphere. However, uninhabitable region is expected to be exist in the deep subsurface biosphere, of which extent and constraining factor(s) have still remained largly unknown. Scientific ocean drilling have revealed that microbial communities in sediments are generally phylogenetically distinct from known spieces isolated from the Earth's surface biosphere, and hence metabolic functions of the deep subseafloor life remain unknown. In addition, activity of subseafloor microbial cells are thought to be extraordinally slow, as indicated by limited supply of neutrient and energy substrates. To understand the limits of the Earth's subseafloor biosphere and metabolic functions of microbial populations, detection and quantification of the deeply buried microbial cells in geological habitats are fundamentary important. Using newly developed cell separation techniques as well as an discriminative cell detection system, the current quantification limit of sedimentary microbial cells approaches to 102 cells/cm3. These techniques allow not only to assess very small microbial population close to the subsurface biotic fringe, but also to separate and sort the target cells using flow cytometric cell sorter. Once the deep subseafloor microbial cells are detached from mineral grains and sorted, it opens new windows to subsequent molecular ecological and element/isotopic analyses. With a combined use of nano-scale secondary ion masspectrometry (NanoSIMS) and stable isotope-probing techniques, it is possible to detect and measure activity of substrate incorporation into biomass, even for extremely slow metabolic processes such as uncharacteriszed deep subseafloor life. For example, it was evidenced by NanoSIMS that at least over 80% of microbial cells at ~200 meters-deep, 460,000-year-old sedimentary habitat are indeed live, which substrate incooporation was found to be low (10-15 gC/cell/day) even under the lab incubation condition. Also microbial activity in ultraoligotrophic biosphere samples such as the South Pacific Gyre (i.e., IODP Expeditions 329) will be shown. Our results demonstrates metabolic potential of microbes that have been survived for geological timescale in extremely starved condition.

Short-term effect of Keyes' approach to periodontal therapy compared with modified Widman flap surgery.

PubMed

Whitehead, S P; Watts, T L

1987-11-01

Keyes' method of non-surgical therapy was compared with modified Widman flap surgery in 9 patients with symmetrical periodontal disease. Following an initial oral hygiene programme, baseline measurements were recorded and paired contralateral areas were subjected randomly to the 2 techniques. 42 teeth receiving surgery were compared with 40 treated by Keyes' method. 6 sites per tooth were scored immediately prior to therapy and 3 months later, using a constant force probe with onlays. Consistent data were recorded for the 6 separate sites, which showed no baseline difference between treatments, slightly greater recession with surgery at 3 months, but no difference between treatments in probing depth and attachment levels. Mean data for individual patients showed similar consistency. Probing depth in deep sites was reduced slightly more with surgery, and there were no differences in bleeding on probing at 3 months. Both techniques gave marked improvements in health. Surprisingly, only 2 subjects preferred Keyes' technique of mechanical therapy, 6 preferred surgery, and 1 had no preference.

Photoswitchable non-fluorescent thermochromic dye-nanoparticle hybrid probes

PubMed Central

Harrington, Walter N.; Haji, Mwafaq R.; Galanzha, Ekaterina I.; Nedosekin, Dmitry A.; Nima, Zeid A.; Watanabe, Fumiya; Ghosh, Anindya; Biris, Alexandru S.; Zharov, Vladimir P.

2016-01-01

Photoswitchable fluorescent proteins with controllable light–dark states and spectral shifts in emission in response to light have led to breakthroughs in the study of cell biology. Nevertheless, conventional photoswitching is not applicable for weakly fluorescent proteins and requires UV light with low depth penetration in bio-tissue. Here we introduce a novel concept of photoswitchable hybrid probes consisting of thermochromic dye and absorbing nanoparticles, in which temperature-sensitive light–dark states and spectral shifts in absorption can be switched through controllable photothermal heating of doped nanoparticles. The proof-of-concept is demonstrated through the use of two different types of temperature-sensitive dyes doped with magnetic nanoparticles and reversibly photoswitched by a near-infrared laser. Photoacoustic imaging revealed the high contrast of these probes, which is sufficient for their visualization in cells and deep tissue. Our results suggest that these new photoswitchable multicolour probes can be used for multimodal cellular diagnostics and potentially for magnetic and photothermal therapy. PMID:27824110

Physical location of the carotenoid biosynthesis genes Psy and β-Lcy in Capsicum annuum (Solanaceae) using heterologous probes from Citrus sinensis (Rutaceae).

PubMed

Andrade-Souza, V; Costa, M G C; Chen, C X; Gmitter, F G; Costa, M A

2011-03-09

Carotenoids are responsible for a range of fruit colors in different hot pepper (Capsicum) varieties, from white to deep red. Color traits are genetically determined by three loci, Y, C1, and C2, which are associated with carotenogenic genes. Although such genes have been localized on genetic maps of Capsicum and anchored in Lycopersicon and Solanum, physical mapping in Capsicum has been restricted to only a few clusters of some multiple copy genes. Heterologous probes from single copy genes have been rarely used. Fluorescent in situ hybridization was performed in Capsicum annuum varieties with different fruit colors, using heterologous probes of Psy and β-Lcy genes obtained from a BAC library of the sweet orange (Citrus sinensis). The probes hybridized in the terminal portion of a chromosome pair, confirming the location of these genes in genetic maps. The hybridized segments showed variation in size in both chromosomes.

Recent Advances in Inorganic Nanoparticle-Based NIR Luminescence Imaging: Semiconductor Nanoparticles and Lanthanide Nanoparticles.

PubMed

Kim, Dokyoon; Lee, Nohyun; Park, Yong Il; Hyeon, Taeghwan

2017-01-18

Several types of nanoparticle-based imaging probes have been developed to replace conventional luminescent probes. For luminescence imaging, near-infrared (NIR) probes are useful in that they allow deep tissue penetration and high spatial resolution as a result of reduced light absorption/scattering and negligible autofluorescence in biological media. They rely on either an anti-Stokes or a Stokes shift process to generate luminescence. For example, transition metal-doped semiconductor nanoparticles and lanthanide-doped inorganic nanoparticles have been demonstrated as anti-Stokes shift-based agents that absorb NIR light through two- or three-photon absorption process and upconversion process, respectively. On the other hand, quantum dots (QDs) and lanthanide-doped nanoparticles that emit in NIR-II range (∼1000 to ∼1350 nm) were suggested as promising Stokes shift-based imaging agents. In this topical review, we summarize and discuss the recent progress in the development of inorganic nanoparticle-based luminescence imaging probes working in NIR range.

Development of scanning graphene Hall probes for magnetic microscopy

NASA Astrophysics Data System (ADS)

Schaefer, Brian T.; Wang, Lei; McEuen, Paul L.; Nowack, Katja C.

We discuss our progress on developing scanning Hall probes fabricated from hexagonal boron nitride (hBN)-encapsulated graphene, with the goal to image magnetic fields with submicron resolution. In contrast to scanning superconducting quantum interference device (SQUID) microscopy, this technique is compatible with a large applied magnetic field and not limited to cryogenic temperatures. The field sensitivity of a Hall probe depends inversely on carrier density, while the primary source of noise in the measurement is Johnson noise originating from the device resistance. hBN-encapsulated graphene demonstrates high carrier mobility at low carrier densities, therefore making it an ideal material for sensitive Hall probes. Furthermore, engineering the dielectric environment of graphene by encapsulating in hBN reduces low-frequency charge noise and disorder from the substrate. We outline our plans for adapting these devices for scanning, including characterization of the point spread function with a scanned current loop and fabrication of a deep-etched structure that enables positioning the sensitive area within 100 nanometers of the sample surface.

Deep UV autofluorescence microscopy for cell biology and tissue histology.

PubMed

Jamme, Frédéric; Kascakova, Slavka; Villette, Sandrine; Allouche, Fatma; Pallu, Stéphane; Rouam, Valérie; Réfrégiers, Matthieu

2013-07-01

Autofluorescence spectroscopy is a powerful tool for molecular histology and for following metabolic processes in biological samples as it does not require labelling. However, at the microscopic scale, it is mostly limited to visible and near infrared excitation of the samples. Several interesting and naturally occurring fluorophores can be excited in the UV and deep UV (DUV), but cannot be monitored in cellulo nor in vivo due to a lack of available microscopic instruments working in this wavelength range. To fulfil this need, we have developed a synchrotron-coupled DUV microspectrofluorimeter which is operational since 2010. An extended selection of endogenous autofluorescent probes that can be excited in DUV, including their spectral characteristics, is presented. The distribution of the probes in various biological samples, including cultured cells, soft tissues, bone sections and maize stems, is shown to illustrate the possibilities offered by this system. In this work we demonstrate that DUV autofluorescence is a powerful tool for tissue histology and cell biology. To fulfil this need, we have developed a synchrotron-coupled DUV microspectrofluorimeter which is operational since 2010. An extended selection of endogenous autofluorescent probes that can be excited in DUV, including their spectral characteristics, is presented. The distribution of the probes in various biological samples, including cultured cells, soft tissues, bone sections and maize stems, is shown to illustrate the possibilities offered by this system. In this work we demonstrate that DUV autofluorescence is a powerful tool for tissue histology and cell biology. In this work we demonstrate that DUV autofluorescence is a powerful tool for tissue histology and cell biology. © 2013 Société Française des Microscopies and Société de Biologie Cellulaire de France. Published by John Wiley & Sons Ltd.

The GalileoJupiter Probe Doppler Wind Experiment

NASA Astrophysics Data System (ADS)

Atkinson, D. H.

2001-09-01

The GalileoJupiter atmospheric entry probe was launched along with the Galileoorbiter spacecraft from Cape Canaveral in Florida, USA, on October 18, 1989. Following a cruise of greater than six years, the probe arrived at Jupiter on December 7, 1995. During its 57-minute descent, instruments on the probe studied the atmospheric composition and structure, the clouds, lightning, and energy structure of the upper Jovian atmosphere. One of the two radio channels over which the experiment data was transmitted to the orbiter was driven by an ultrastable oscillator. All motions of the probe and orbiter, including the speed of probe descent, Jupiter's rotation, and the atmospheric winds, contributed to a Doppler shift of the probe radio frequency. By accurately measuring the frequency of the probe radio signal, an accurate time history of the probe-orbiter relative motions could be reconstructed. Knowledge of the nominal probe and orbiter trajectories allowed the nominal Doppler shift to be removed from the probe radio frequency leaving a measurable frequency residual arising primarily from the zonal winds in Jupiter's atmosphere, and micromotions of the probe arising from probe spin, swing under the parachute, atmospheric turbulence, and aerodynamic effects. Assuming that the zonal horizontal winds dominate the residual probe motion, a profile of frequency residuals was generated. Inversion of the frequency residuals resulted in the first in situ measurements of the vertical profile of Jupiter's deep zonal winds. It is found that beneath 700 mb, the winds are strong and prograde, rising rapidly to 170 m/s between 1 and 4 bars. Beneath 4 bars to 21 bars, the depth at which the link with the probe was lost, the winds remain constant and strong. When corrections for the high temperatures encountered by the probe are considered, there is no evidence of diminishing or strengthening of the zonal winds in the deepest regions explored by the Galileoprobe. Following the wind recovery, the frequency residuals offer tantalizing clues to microstructure in the atmospheric dynamics, including turbulence and wave motion.

Radial transport of high-energy oxygen ions into the deep inner magnetosphere observed by Van Allen Probes

NASA Astrophysics Data System (ADS)

Mitani, K.; Seki, K.; Keika, K.; Gkioulidou, M.; Lanzerotti, L. J.; Mitchell, D. G.; Kletzing, C.

2017-12-01

It is known that proton is main contributor of the ring current and oxygen ions can make significant contribution during major magnetic storms. Ions are supplied to the ring current by radial transport from the plasma sheet. Convective transport of lower-energy protons and diffusive transport of higher-energy protons were reported to contribute to the storm-time and quiet-time ring current respectively [e.g., Gkioulidou et al., 2016]. However, supply mechanisms of the oxygen ions are not clear. To characterize the supply of oxygen ions to the ring current during magnetic storms, we studied the properties of energetic proton and oxygen ion phase space densities (PSDs) for specific magnetic moment (μ) during the April 23-25, 2013, geomagnetic storm observed by the Van Allen Probes mission. We here report on radial transport of high-energy (μ ≥ 0.5 keV/nT) oxygen ions into the deep inner magnetosphere during the late main phase of the magnetic storm. Since protons show little change during this period, this oxygen radial transport is inferred to cause the development of the late main phase. Enhancement of poloidal magnetic fluctuations is simultaneously observed. We estimated azimuthal mode number ≤5 by using cross wavelet analysis with ground-based observation of IMAGE ground magnetometers. The fluctuations can resonate with drift and bounce motions of the oxygen ions. The results suggest that combination of the drift and drift-bounce resonances is responsible for the radial transport of high-energy oxygen ions into the deep inner magnetosphere. We also report on the radial transport of the high-energy oxygen ions into the deep inner magnetosphere during other magnetic storms.

RELICS of the Cosmic Dawn

NASA Astrophysics Data System (ADS)

Bradac, Marusa; Coe, Dan; Strait, Victoria; Salmon, Brett; Hoag, Austin; Bradley, Larry; Ryan, Russell; Dawson, Will; Zitrin, Adi; Jones, Christine; Sharon, Keren; Trenti, Michele; Stark, Daniel; Oesch, Pascal; Lam, Danel; Carrasco Nunez, Daniela Patricia; Paterno-Mahler, Rachel; Frye, Brenda

2018-05-01

When did galaxies start forming stars? What is the role of distant galaxies in galaxy formation models and epoch of reionization? Recent observations indicate at least two critical puzzles in these studies. (1) First galaxies might have started forming stars earlier than previously thought (<400Myr after the Big Bang). (2) It is still unclear what is their star formation history and whether these galaxies can reionize the Universe. Accurate knowledge of stellar masses, ages, and star formation rates at this epoch requires measuring both rest-frame UV and optical light, which only Spitzer and HST can probe at z 6-11 for a large enough sample of typical galaxies. To address this cosmic puzzle, we propose to complete deep Spitzer imaging of the fields behind the 10 most powerful cosmic telescopes selected using HST, Spitzer, and Planck data from the RELICS and SRELICS programs (Reionization Lensing Cluster Survey; 41 clusters, 190 HST orbits, 440 Spitzer hours). 6 clusters out of 10 are still lacking deep data. This proposal will be a valuable Legacy complement to the existing IRAC deep surveys, and it will open up a new parameter space by probing the ordinary yet magnified population with much improved sample variance. The program will allow us to study stellar properties of a large number, 60 galaxies at z 6-11. Deep Spitzer data will be crucial to unambiguously measure their stellar properties (age, SFR, M*). Finally this proposal will establish the presence (or absence) of an unusually early established stellar population, as was recently observed in MACS1149JD at z 9. If confirmed in a larger sample, this result will require a paradigm shift in our understanding of the earliest star formation.

Results from the DOLCE (Deep Space Optical Link Communications Experiment) project

NASA Astrophysics Data System (ADS)

Baister, Guy; Kudielka, Klaus; Dreischer, Thomas; Tüchler, Michael

2009-02-01

Oerlikon Space AG has since 1995 been developing the OPTEL family of optical communications terminals. The optical terminals within the OPTEL family have been designed so as to be able to position Oerlikon Space for future opportunities open to this technology. These opportunities range from commercial optical satellite crosslinks between geostationary (GEO) satellites, deep space optical links between planetary probes and the Earth, as well as optical links between airborne platforms (either between the airborne platforms or between a platform and GEO satellite). The OPTEL terminal for deep space applications has been designed as an integrated RF-optical terminal for telemetry links between the science probe and Earth. The integrated architecture provides increased TM link capacities through the use of an optical link, while spacecraft navigation and telecommand are ensured by the classical RF link. The optical TM link employs pulsed laser communications operating at 1058nm to transmit data using PPM modulation to achieve a robust link to atmospheric degradation at the optical ground station. For deep space links from Lagrange (L1 / L2) data rates of 10 - 20 Mbps can be achieved for the same spacecraft budgets (mass and power) as an RF high gain antenna. Results of an inter-island test campaign to demonstrate the performance of the pulsed laser communications subsystem employing 32-PPM for links through the atmosphere over a distance of 142 km are presented. The transmitter of the communications subsystem is a master oscillator power amplifier (MOPA) employing a 1 W (average power) amplifier and the receiver a Si APD with a measured sensitivity of -70.9 dBm for 32-PPM modulation format at a user data rate of 10 Mbps and a bit error rate (BER) of 10-6.

Probing the Deep: Theory and History

ERIC Educational Resources Information Center

Beadie, Nancy

2011-01-01

Professor Tamura, in her paper "Narrative History and Theory," poses an issue with which the author has lately wrestled. She reviews some of the challenges to the tradition of narrative history presented by "social-scientifically oriented historians" like Fernand Braudel and "analytic philosophers" like Hayden White in the 1960s and 1970s, and…

Literature-Related Discovery (LRD)

DTIC Science & Technology

2007-11-01

accepted) water purification literature. The annular region between the inner and outer circles represents literatures related directly and...procedures (thalamotomy and pallidotomy) destroy regions of the brain that produce the uncontrolled spasmodic movements in PD patients [11]. A...more recent procedure, deep brain stimulation, sends electricity through a probe to normalize electrical activity in the brain region , reversing the

Volumetric imaging of fast biological dynamics in deep tissue via wavefront engineering

NASA Astrophysics Data System (ADS)

Kong, Lingjie; Tang, Jianyong; Cui, Meng

2016-03-01

To reveal fast biological dynamics in deep tissue, we combine two wavefront engineering methods that were developed in our laboratory, namely optical phase-locked ultrasound lens (OPLUL) based volumetric imaging and iterative multiphoton adaptive compensation technique (IMPACT). OPLUL is used to generate oscillating defocusing wavefront for fast axial scanning, and IMPACT is used to compensate the wavefront distortions for deep tissue imaging. We show its promising applications in neuroscience and immunology.

Electronic Components and Systems for Cryogenic Space Applications

NASA Technical Reports Server (NTRS)

Patterson, R. L.; Hammoud, A.; Dickman, J. E.; Gerber, S.; Elbuluk, M. E.; Overton, E.

2001-01-01

Electronic components and systems capable of operation at cryogenic temperatures are anticipated in many future NASA space missions such as deep space probes and planetary surface exploration. For example, an unheated interplanetary probe launched to explore the rings of Saturn would reach an average temperature near Saturn of about - 183 C. In addition to surviving the deep space harsh environment, electronics capable of low temperature operation would contribute to improving circuit performance, increasing system efficiency, and reducing payload development and launch costs. Terrestrial applications where components and systems must operate in low temperature environments include cryogenic instrumentation, superconducting magnetic energy storage, magnetic levitation transportation system, and arctic exploration. An on-going research and development program at the NASA Glenn Research Center focuses on the development of reliable electronic devices and efficient power systems capable of surviving in low temperature environments. An overview of the program will be presented in this paper. A description of the low temperature test facilities along with selected data obtained from in-house component testing will also be discussed. Ongoing research activities that are being performed in collaboration with various organizations will also be presented.

Video-rate functional photoacoustic microscopy at depths

NASA Astrophysics Data System (ADS)

Wang, Lidai; Maslov, Konstantin; Xing, Wenxin; Garcia-Uribe, Alejandro; Wang, Lihong V.

2012-10-01

We report the development of functional photoacoustic microscopy capable of video-rate high-resolution in vivo imaging in deep tissue. A lightweight photoacoustic probe is made of a single-element broadband ultrasound transducer, a compact photoacoustic beam combiner, and a bright-field light delivery system. Focused broadband ultrasound detection provides a 44-μm lateral resolution and a 28-μm axial resolution based on the envelope (a 15-μm axial resolution based on the raw RF signal). Due to the efficient bright-field light delivery, the system can image as deep as 4.8 mm in vivo using low excitation pulse energy (28 μJ per pulse, 0.35 mJ/cm2 on the skin surface). The photoacoustic probe is mounted on a fast-scanning voice-coil scanner to acquire 40 two-dimensional (2-D) B-scan images per second over a 9-mm range. High-resolution anatomical imaging is demonstrated in the mouse ear and brain. Via fast dual-wavelength switching, oxygen dynamics of mouse cardio-vasculature is imaged in realtime as well.

Mapping wetland species and the impact of oil from the Deep Horizon using the Airborne/Visible Imaging Spectrometer and Multiple Endmember Spectral Mixture Analysis

NASA Astrophysics Data System (ADS)

Roberts, D. A.; Beland, M.; Kokaly, R. F.; Couvillion, B.; Ustin, S.; Peterson, S.

2011-12-01

Between April 20, 2010 and July 15, 2010 an estimated 4.4 million barrels of oil leaked from the Maconda well, making the Deep Horizon oil spill the largest in US history. In response to a need to determine the distribution of wetland plant species and quantify their condition prior to, during and after oil reached the shore, the Airborne/Visible Infrared Imaging Spectrometer (AVIRIS) was deployed multiple times in the gulf on high altitude and low altitude airborne platforms. Significant research questions included 1) What is the distribution of key wetland species in the impacted area?; 2) which areas were impacted by oil, when and to what extent?; 3) how much oil must be present to be detected in various cover types? and 4) which wetland species are more sensitive to oil? In an effort to answer some of these questions, we applied Multiple Endmember Spectral Mixture Analysis (MESMA) to AVIRIS data acquired prior to significant impacts in May, 2010 and after oil had reached wetlands in late summer and fall, 2010. Reference polygons for species dominants were located on the images and used to build a spectral library for all dominant wetland species and surface types. This spectral library was augmented by field spectra, acquired using a contact probe for senesced plants materials and beach sands. Spectra of heavily oiled surfaces were identified using the Hydrocarbon Index to identify potential oil endmembers and the Cellulose Absorption Index to discriminate oil from Non-photosynthetic Vegetation (NPV). Wetland species and cover fractions for Green Vegetation (GV), NPV, soils/beaches, oil and water were mapped using MESMA applied to images acquired in the Birds Foot Delta, Chandeleur Islands and Barataria Bay. Species maps, showing dominant species such as Phragmites australis, Spartina alternifolia and S. patens proved to be accurate. OIl was mapped along coastal areas of Barataria Bay, expressed as high oil fractions. However, significant confusion was also observed between oiled vegetation and senesced vegetation, either resulting from oil-induced mortality or natural senescence.

Footprint of Deepwater Horizon blowout impact to deep-water coral communities

PubMed Central

Fisher, Charles R.; Hsing, Pen-Yuan; Kaiser, Carl L.; Yoerger, Dana R.; Roberts, Harry H.; Shedd, William W.; Cordes, Erik E.; Shank, Timothy M.; Berlet, Samantha P.; Saunders, Miles G.; Larcom, Elizabeth A.; Brooks, James M.

2014-01-01

On April 20, 2010, the Deepwater Horizon (DWH) blowout occurred, releasing more oil than any accidental spill in history. Oil release continued for 87 d and much of the oil and gas remained in, or returned to, the deep sea. A coral community significantly impacted by the spill was discovered in late 2010 at 1,370 m depth. Here we describe the discovery of five previously unknown coral communities near the Macondo wellhead and show that at least two additional coral communities were impacted by the spill. Although the oil-containing flocullent material that was present on corals when the first impacted community was discovered was largely gone, a characteristic patchy covering of hydrozoans on dead portions of the skeleton allowed recognition of impacted colonies at the more recently discovered sites. One of these communities was 6 km south of the Macondo wellhead and over 90% of the corals present showed the characteristic signs of recent impact. The other community, 22 km southeast of the wellhead between 1,850 and 1,950 m depth, was more lightly impacted. However, the discovery of this site considerably extends the distance from Macondo and depth range of significant impact to benthic macrofaunal communities. We also show that most known deep-water coral communities in the Gulf of Mexico do not appear to have been acutely impacted by the spill, although two of the newly discovered communities near the wellhead apparently not impacted by the spill have been impacted by deep-sea fishing operations. PMID:25071200

GALEX Study of the UV Variability of Nearby Galaxies and a Deep Probe of the UV Luminosity Function

NASA Technical Reports Server (NTRS)

Schlegel, Eric

2005-01-01

The proposal has two aims - a deep exposure of NGC 300, about a factor of 10 deeper than the GALEX all-sky survey; and an examination of the UV variability. The data were received just prior to a series of proposal deadlines in early spring. A subsequent analysis delay includes a move from SAO to the University of Texas - San Antonio. Nevertheless, we have merged the data into a single deep exposure as well as undertaking a preliminary examination of the variability. No UV halo is present as detected in the GALEX observation of M83. No UV bursts are visible; however a more stringent limit will only be obtained through a differencing of the sub-images. Papers: we expect 2 papers at about 12 pages/paper to flow from this project. The first paper will report on the time variability while the second will focus on the deep UV image obtained from stacking the individual observations.

Designed Er(3+)-singly doped NaYF4 with double excitation bands for simultaneous deep macroscopic and microscopic upconverting bioimaging.

PubMed

Wen, Xuanyuan; Wang, Baoju; Wu, Ruitao; Li, Nana; He, Sailing; Zhan, Qiuqiang

2016-06-01

Simultaneous deep macroscopic imaging and microscopic imaging is in urgent demand, but is challenging to achieve experimentally due to the lack of proper fluorescent probes. Herein, we have designed and successfully synthesized simplex Er(3+)-doped upconversion nanoparticles (UCNPs) with double excitation bands for simultaneous deep macroscopic and microscopic imaging. The material structure and the excitation wavelength of Er(3+)-singly doped UCNPs were further optimized to enhance the upconversion emission efficiency. After optimization, we found that NaYF4:30%Er(3+)@NaYF4:2%Er(3+) could simultaneously achieve efficient two-photon excitation (2PE) macroscopic tissue imaging and three-photon excitation (3PE) deep microscopic when excited by 808 nm continuous wave (CW) and 1480 nm CW lasers, respectively. In vitro cell imaging and in vivo imaging have also been implemented to demonstrate the feasibility and potential of the proposed simplex Er(3+)-doped UCNPs as bioprobe.

When beauty is only skin deep; optimizing the sensitivity of specular neutron reflectivity for probing structure beneath the surface of thin filmsa)

NASA Astrophysics Data System (ADS)

Majkrzak, Charles F.; Carpenter, Elisabeth; Heinrich, Frank; Berk, Norman F.

2011-11-01

Specular neutron reflectometry has become an established probe of the nanometer scale structure of materials in thin film and multilayered form. It has contributed especially to our understanding of soft condensed matter of interest in polymer science, organic chemistry, and biology and of magnetic hard condensed matter systems. In this paper we examine a number of key factors which have emerged that can limit the sensitivity of neutron reflection as such a probe. Among these is loss of phase information, and we discuss how knowledge about material surrounding a film of interest can be applied to help resolve the problem. In this context we also consider what role the quantum phenomenon of interaction-free measurement might play in enhancing the statistical efficiency for obtaining reflectivity or transmission data.

Propulsion Utilizing Laser-Driven Ponderomotive Fields for Deep-Space Missions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Williams, George J.; Gilland, James H.

The generation of large amplitude electric fields in plasmas by high-power lasers has been studied for several years in the context of high-energy particle acceleration. Fields on the order of GeV/m are generated in the plasma wake of the laser by non-linear ponderomotive forces. The laser fields generate longitudinal and translational electron plasma waves with phase velocities close to the speed of light. These fields and velocities offer the potential to revolutionize spacecraft propulsion, leading to extended deep space robotic probes. Based on these initial calculations, plasma acceleration by means of laser-induced ponderomotive forces appears to offer significant potential formore » spacecraft propulsion. Relatively high-efficiencies appear possible with proper beam conditioning, resulting in an order of magnitude more thrust than alternative concepts for high I{sub SP} (>10{sup 5} s) and elimination of the primary life-limiting erosion phenomena associated with conventional electric propulsion systems. Ponderomotive propulsion readily lends itself to beamed power which might overcome some of the constraints of power-limited propulsion concepts. A preliminary assessment of the impact of these propulsion systems for several promising configurations on mission architectures has been conducted. Emphasizing interstellar and interstellar-precursor applications, performance and technical requirements are identified for a number of missions. The use of in-situ plasma and gas for propellant is evaluated as well.« less

Chronic neural probe for simultaneous recording of single-unit, multi-unit, and local field potential activity from multiple brain sites

NASA Astrophysics Data System (ADS)

Pothof, F.; Bonini, L.; Lanzilotto, M.; Livi, A.; Fogassi, L.; Orban, G. A.; Paul, O.; Ruther, P.

2016-08-01

Objective. Drug resistant focal epilepsy can be treated by resecting the epileptic focus requiring a precise focus localisation using stereoelectroencephalography (SEEG) probes. As commercial SEEG probes offer only a limited spatial resolution, probes of higher channel count and design freedom enabling the incorporation of macro and microelectrodes would help increasing spatial resolution and thus open new perspectives for investigating mechanisms underlying focal epilepsy and its treatment. This work describes a new fabrication process for SEEG probes with materials and dimensions similar to clinical probes enabling recording single neuron activity at high spatial resolution. Approach. Polyimide is used as a biocompatible flexible substrate into which platinum electrodes and leads are integrated with a minimal feature size of 5 μm. The polyimide foils are rolled into the cylindrical probe shape at a diameter of 0.8 mm. The resulting probe features match those of clinically approved devices. Tests in saline solution confirmed the probe stability and functionality. Probes were implanted into the brain of one monkey (Macaca mulatta), trained to perform different motor tasks. Suitable configurations including up to 128 electrode sites allow the recording of task-related neuronal signals. Main results. Probes with 32 and 64 electrode sites were implanted in the posterior parietal cortex. Local field potentials and multi-unit activity were recorded as early as one hour after implantation. Stable single-unit activity was achieved for up to 26 days after implantation of a 64-channel probe. All recorded signals showed modulation during task execution. Significance. With the novel probes it is possible to record stable biologically relevant data over a time span exceeding the usual time needed for epileptic focus localisation in human patients. This is the first time that single units are recorded along cylindrical polyimide probes chronically implanted 22 mm deep into the brain of a monkey, which suggests the potential usefulness of this probe for human applications.

Chronic neural probe for simultaneous recording of single-unit, multi-unit, and local field potential activity from multiple brain sites.

PubMed

Pothof, F; Bonini, L; Lanzilotto, M; Livi, A; Fogassi, L; Orban, G A; Paul, O; Ruther, P

2016-08-01

Drug resistant focal epilepsy can be treated by resecting the epileptic focus requiring a precise focus localisation using stereoelectroencephalography (SEEG) probes. As commercial SEEG probes offer only a limited spatial resolution, probes of higher channel count and design freedom enabling the incorporation of macro and microelectrodes would help increasing spatial resolution and thus open new perspectives for investigating mechanisms underlying focal epilepsy and its treatment. This work describes a new fabrication process for SEEG probes with materials and dimensions similar to clinical probes enabling recording single neuron activity at high spatial resolution. Polyimide is used as a biocompatible flexible substrate into which platinum electrodes and leads are integrated with a minimal feature size of 5 μm. The polyimide foils are rolled into the cylindrical probe shape at a diameter of 0.8 mm. The resulting probe features match those of clinically approved devices. Tests in saline solution confirmed the probe stability and functionality. Probes were implanted into the brain of one monkey (Macaca mulatta), trained to perform different motor tasks. Suitable configurations including up to 128 electrode sites allow the recording of task-related neuronal signals. Probes with 32 and 64 electrode sites were implanted in the posterior parietal cortex. Local field potentials and multi-unit activity were recorded as early as one hour after implantation. Stable single-unit activity was achieved for up to 26 days after implantation of a 64-channel probe. All recorded signals showed modulation during task execution. With the novel probes it is possible to record stable biologically relevant data over a time span exceeding the usual time needed for epileptic focus localisation in human patients. This is the first time that single units are recorded along cylindrical polyimide probes chronically implanted 22 mm deep into the brain of a monkey, which suggests the potential usefulness of this probe for human applications.

Clinical effect of azithromycin as an adjunct to non-surgical treatment of chronic periodontitis: a meta-analysis of randomized controlled clinical trials.

PubMed

Zhang, Z; Zheng, Y; Bian, X

2016-06-01

The results of recent published studies focusing on the effect of azithromycin as an adjunct to scaling and root planing (SRP) in the treatment of chronic periodontitis are inconsistent. We conducted a meta-analysis of randomized controlled clinical trials to examine the effect of azithromycin combined with SRP on periodontal clinical parameters as compared to SRP alone. An electronic search was carried out on Pubmed, Embase and the Cochrane Central Register of Controlled Trials from their earliest records through December 28, 2014 to identify studies that met pre-stated inclusion criteria. Reference lists of retrieved articles were also reviewed. Data were extracted independently by two authors. Either a fixed- or random-effects model was used to calculate the overall effect sizes of azithromycin on probing depth, attachment level (AL) and bleeding on probing (BOP). Heterogeneity was evaluated using the Q test and I(2) statistic. Publication bias was evaluated by Begg's test and Egger's test. A total of 14 trials were included in the meta-analysis. Compared with SRP alone, locally delivered azithromycin plus SRP statistically significantly reduced probing depth by 0.99 mm (95% CI 0.42-1.57) and increased AL by 1.12 mm (95% CI 0.31-1.92). In addition, systemically administered azithromycin plus SRP statistically significantly reduced probing depth by 0.21 mm (95% CI 0.12-0.29), BOP by 4.50% (95% CI 1.45-7.56) and increased AL by 0.23 mm (95% CI 0.07-0.39). Sensitivity analysis yielded similar results. No evidence of publication bias was observed. The additional benefit of systemic azithromycin was shown at the initially deep probing depth sites, but not at shallow or moderate sites. The overall effect sizes of systemic azithromycin showed a tendency to decrease with time, and meta-regression analysis suggested a negative relation between the length of follow-up and net change in probing depth (r = -0.05, p = 0.02). This meta-analysis provides further evidence that azithromycin used as an adjunct to SRP significantly improves the efficacy of non-surgical periodontal therapy on reducing probing depth, BOP and improving AL, particularly at the initially deep probing depth sites. © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

The Great Observatories Origins Deep Survey Spitzer Legacy Science Program

NASA Astrophysics Data System (ADS)

Dickinson, M.; GOODS Team

2005-12-01

The Great Observatories Origins Deep Survey (GOODS) is a multiwavelength anthology of deep field programs using NASA's Great Observatories and the most powerful ground-based facilities to create a public data resource for studying the formation and evolution of galaxies and active galactic nuclei (AGN) throughout cosmic history. GOODS incorporates a Spitzer Legacy Program, which has obtained the deepest observations with that telescope at 3.6 to 24 microns. The Spitzer/IRAC data detect the rest-frame near-infrared light from galaxies out to z ˜ 6, providing valuable information on their stellar populations and masses. The MIPS 24μ m data are a sensitive probe of re-emitted energy from dust-obscured star formation and AGN out to z ˜ 3. I will very briefly introduce the survey and summarize science highlights from the Spitzer data.

Probing reservoir-triggered earthquakes in Koyna, India, through scientific deep drilling

USGS Publications Warehouse

Gupta, H.; Nayak, Shailesh; Ellsworth, William L.; Rao, Y. J. B.; Rajan, S.; Bansal, B.K.; Purnachandra Rao, N.; Roy, S.; Arora, K.; Mohan, R.; Tiwari, V. M.; Satyanarayana, H. V. S.; Patro, P. K.; Shashidhar, D.; Mallika, K.

2014-01-01

We report here the salient features of the recently concluded International Continental Scientific Drilling Program (ICDP) workshop in Koyna, India. This workshop was a sequel to the earlier held ICDP workshop in Hyderabad and Koyna in 2011. A total of 49 experts (37 from India and 12 from 8 other countries) spent 3 days reviewing the work carried out during the last 3 years based on the recommendations of the 2011 workshop and suggesting the future course of action, including detailed planning for a full deep drilling proposal in Koyna, India. It was unanimously concluded that Koyna is one of the best sites anywhere in the world to investigate genesis of triggered earthquakes from near-field observations. A broad framework of the activities for the next phase leading to deep drilling has been worked out.

Determining in-situ thermal conductivity of coarse textured materials through numerical analysis of thermal

NASA Astrophysics Data System (ADS)

Saito, H.; Hamamoto, S.; Moldrup, P.; Komatsu, T.

2013-12-01

Ground source heat pump (GSHP) systems use ground or groundwater as a heat/cooling source, typically by circulating anti-freezing solution inside a vertically installed closed-loop tube known as a U-tube to transfer heat to/from the ground. Since GSHP systems are based on renewable energy and can achieve much higher coefficient of performance (COP) than conventional air source heat pump systems, use of GSHP systems has been rapidly increasing worldwide. However, environmental impacts by GSHP systems including thermal effects on subsurface physical-chemical and microbiological properties have not been fully investigated. To rigorously assess GSHP impact on the subsurface environment, ground thermal properties including thermal conductivity and heat capacity need to be accurately characterized. Ground thermal properties were investigated at two experimental sites at Tokyo University of Agriculture and Technology (TAT) and Saitama University (SA), both located in the Kanto area of Japan. Thermal properties were evaluated both by thermal probe measurements on boring core samples and by performing in-situ Thermal Response Tests (TRT) in 50-80 m deep U-tubes. At both TAT and SU sites, heat-pulse probe measurements gave unrealistic low thermal conductivities for coarse textured materials (dominated by particles > 75 micrometers). Such underestimation can be partly due to poor contact between probe and porous material and partly to markedly decreasing sample water content during drilling, carrying, and storing sandy/gravelly samples. A more reliable approach for estimating in-situ thermal conductivity of coarse textured materials is therefore needed, and may be based on the commonly used TRT test. However, analyses of TRT data is typically based on Kelvin's line source model and provides an average (effective) thermal property for the whole soil profile around the U-tube but not for each geological layer. The main objective of this study was therefore to develop a method for estimating thermal conductivity values of coarse textured layers by numerically analyzing TRT data. A numerical technique combining three-dimensional conductive heat transport and one-dimensional convective heat transport to simulate heat exchange processes between the U-tube and the ground was used. In the numerical simulations, the thermal conductivities for the fine textured layers were kept at the probe-measured values, while the thermal conductivity for the coarse textured layers (constituting around half of the profile depth at both sites) was calibrated. The numerically-based method yielded more reasonable thermal conductivity values for the coarse-textured materials at both TAT and SU sites as compared to the heat pulse probe measurements, while the temperature changes of the heat carry fluid inside the U-tubes were also well simulated.

A resumable two-photon fluorescent probe for Cu2+ and S2- based on magnetic silica core-shell Fe3O4@SiO2 nanoparticles and its application in bioimaging.

PubMed

Jiang, Huie; Liu, Yan; Luo, Weifang; Wang, Yujiao; Tang, Xiaoliang; Dou, Wei; Cui, Yumei; Liu, Weisheng

2018-07-19

A two-photon fluorescent probe for Cu 2+ and S 2- has been strategically prepared with naphthalimide derivative platform (NPE) covalently grafted onto the surface of magnetic core-shell Fe 3 O 4 @SiO 2 nanoparticles. The probe (NPE-Fe 3 O 4 @SiO 2 ) exhibits selective response to Cu 2+ with enhanced fluorescence and efficient separation of Cu 2+ with external magnetic field. The consequent product NPE-Fe 3 O 4 @SiO 2 -Cu of NPE-Fe 3 O 4 @SiO 2 and Cu 2+ can work as an excellent sensor for S 2- by removing Cu 2+ from the complex with fluorescence decreased, recovering the fluorescence of the probe. Therefore, the constituted Off-On-Off type fluorescence monitoring system means the probe is resumable. Moreover, the probe has been used to quantitatively detect Cu 2+ and S 2- with low detection limits, which are 0.28 μM and 0.12 μM, respectively. Furthermore, the probe shows low cytotoxicity and excellent membrane permeability, which has been successfully applied for monitoring Cu 2+ and S 2- in living cells and imaging Cu 2+ in deep-tissue with two-photon excited fluorescence. Copyright © 2018. Published by Elsevier B.V.

Optogenetic activation of neocortical neurons in vivo with a sapphire-based micro-scale LED probe.

PubMed

McAlinden, Niall; Gu, Erdan; Dawson, Martin D; Sakata, Shuzo; Mathieson, Keith

2015-01-01

Optogenetics has proven to be a revolutionary technology in neuroscience and has advanced continuously over the past decade. However, optical stimulation technologies for in vivo need to be developed to match the advances in genetics and biochemistry that have driven this field. In particular, conventional approaches for in vivo optical illumination have a limitation on the achievable spatio-temporal resolution. Here we utilize a sapphire-based microscale gallium nitride light-emitting diode (μLED) probe to activate neocortical neurons in vivo. The probes were designed to contain independently controllable multiple μLEDs, emitting at 450 nm wavelength with an irradiance of up to 2 W/mm(2). Monte-Carlo stimulations predicted that optical stimulation using a μLED can modulate neural activity within a localized region. To validate this prediction, we tested this probe in the mouse neocortex that expressed channelrhodopsin-2 (ChR2) and compared the results with optical stimulation through a fiber at the cortical surface. We confirmed that both approaches reliably induced action potentials in cortical neurons and that the μLED probe evoked strong responses in deep neurons. Due to the possibility to integrate many optical stimulation sites onto a single shank, the μLED probe is thus a promising approach to control neurons locally in vivo.

Motion streaks in fast motion rivalry cause orientation-selective suppression.

PubMed

Apthorp, Deborah; Wenderoth, Peter; Alais, David

2009-05-14

We studied binocular rivalry between orthogonally translating arrays of random Gaussian blobs and measured the strength of rivalry suppression for static oriented probes. Suppression depth was quantified by expressing monocular probe thresholds during dominance relative to thresholds during suppression. Rivalry between two fast motions or two slow motions was compared in order to test the suggestion that fast-moving objects leave oriented "motion streaks" due to temporal integration (W. S. Geisler, 1999). If fast motions do produce motion streaks, then fast motion rivalry might also entail rivalry between the orthogonal streak orientations. We tested this using a static oriented probe that was aligned either parallel to the motion trajectory (hence collinear with the "streaks") or was orthogonal to the trajectory, predicting that rivalry suppression would be greater for parallel probes, and only for rivalry between fast motions. Results confirmed that suppression depth did depend on probe orientation for fast motion but not for slow motion. Further experiments showed that threshold elevations for the oriented probe during suppression exhibited clear orientation tuning. However, orientation-tuned elevations were also present during dominance, suggesting within-channel masking as the basis of the extra-deep suppression. In sum, the presence of orientation-dependent suppression in fast motion rivalry is consistent with the "motion streaks" hypothesis.

Deep Impact Delta II Launch Vehicle Cracked Thick Film Coating on Electronic Packages Technical Consultation Report

NASA Technical Reports Server (NTRS)

Cameron, Kenneth D.; Kichak, Robert A.; Piascik, Robert S.; Leidecker, Henning W.; Wilson, Timmy R.

2009-01-01

The Deep Impact spacecraft was launched on a Boeing Delta II rocket from Cape Canaveral Air Force Station (CCAFS) on January 12, 2005. Prior to the launch, the Director of the Office of Safety and Mission Assurance (OS&MA) requested the NASA Engineering and Safety Center (NESC) lead a team to render an independent opinion on the rationale for flight and the risk code assignments for the hazard of cracked Thick Film Assemblies (TFAs) in the E-packages of the Delta II launch vehicle for the Deep Impact Mission. The results of the evaluation are contained in this report.

Visualization of upconverting nanoparticles in strongly scattering media

PubMed Central

Khaydukov, E. V.; Semchishen, V. A.; Seminogov, V. N.; Nechaev, A. V.; Zvyagin, A. V.; Sokolov, V. I.; Akhmanov, A. S.; Panchenko, V. Ya.

2014-01-01

Optical visualization systems are needed in medical applications for determining the localization of deep-seated luminescent markers in biotissues. The spatial resolution of such systems is limited by the scattering of the tissues. We present a novel epi-luminescent technique, which allows a 1.8-fold increase in the lateral spatial resolution in determining the localization of markers lying deep in a scattering medium compared to the traditional visualization techniques. This goal is attained by using NaYF4:Yb3+Tm3+@NaYF4 core/shell nanoparticles and special optical fiber probe with combined channels for the excitation and detection of anti-Stokes luminescence signals. PMID:24940552

Influence of seasonal climatic variability on shallow infiltration at Yucca Mountain

USGS Publications Warehouse

Hevesi, Joseph A.; Flint, Alan L.

1993-01-01

To analyze infiltration and the redistribution of moisture in alluvial deposits at Yucca Mountain, water content profiles at a 13.5 m deep borehole were measured at monthly intervals using a neutron moisture probe. Increases in water content to a maximum depth of 1.8 m in response to winter season precipitation were noted. Below a depth of 1.8 m, a gradual drying trend was indicated. A simulation study showed that, although small amounts of water may be percolating through the deep nonwetted ones of the profile, the influence of climatic variability on infiltration through thick alluvial deposits at Yucca Mountain is greatly mitigated by evapotranspiration.

Deep drilling at the Siljan Ring impact structure: oxygen-isotope geochemistry of granite

USGS Publications Warehouse

Komor, S.C.; Valley, J.W.

1990-01-01

The Siljan Ring is a 362-Ma-old impact structure formed in 1700-Ma-old I-type granites. A 6.8-km-deep borehole provides a vertical profile through granites and isolated horizontal diabase sills. Fluid-inclusion thermometry, and oxygen-isotope compositions of vein quartz, granite, diabase, impact melt, and pseudotachylite, reveal a complex history of fluid activity in the Siljan Ring, much of which can be related to the meteorite impact. In granites from the deep borehole, ??18O values of matrix quartz increase with depth from near 8.0 at the surface to 9.5??? at 5760 m depth. In contrast, feldspar ??18O values decrease with depth from near 10 at the surface to 7.1??? at 5760 m, forming a pattern opposite to the one defined by quartz isotopic compositions. Values of ??18O for surface granites outside the impact structure are distinct from those in near-surface samples from the deep borehole. In the deep borehole, feldspar coloration varies from brick-red at the surface to white at 5760 m, and the abundances of crack-healing calcite and other secondary minerals decrease over the same interval. Superimposed on the overall decrease in alteration intensity with depth are localized fracture zones at 4662, 5415, and 6044 m depth that contain altered granites, and which provided pathways for deep penetration of surface water. The antithetic variation of quartz and feldspar ??18O values, which can be correlated with mineralogical evidence of alteration, provides evidence for interaction between rocks and impact-heated fluids (100-300?? C) in the upper 2 km of the pluton. Penetration of water to depths below 2 km was restricted by a general decrease in impact-fracturing with depth, and by a 60-m-thick diabase sill at 1500 m depth that may have been an aquitard. At depths below 4 km in the pluton, where water/rock ratios were low, oxygen isotopic compositions preserve evidence for limited high-temperature (>500?? C) exchange between alkali feldspar and fluids. The high-temperature exchange may have been a post-impact event involving impact-heated fluids, or a post-magmatic event. ?? 1990 Springer-Verlag.

The incidence of periodontal defects distal to the maxillary second molar after impacted third molar extraction.

PubMed

Coleman, Michael; McCormick, Adam; Laskin, Daniel M

2011-02-01

This study assessed the incidence of periodontal defects on the distal aspect of maxillary second molars after extraction of impacted maxillary third molars. Subjects enrolled in this institutional review board-approved prospective study consisted of healthy young patients having extraction of at least 1 asymptomatic impacted maxillary third molar adjacent to a second molar. Preoperative periodontal probing data were collected from 4 sites (midbuccal, distobuccal, midpalatal, and distopalatal) on each adjacent second molar, and a similar probing examination was performed at a mean of 6 months postoperatively. All subjects were treated under general anesthesia or conscious sedation by upper-level residents in the outpatient clinic. Twenty subjects with a total of 38 impacted maxillary third molars were treated. There were 9 male subjects (45%) and 11 female subjects (55%), with a mean age of 17 years (range, 14-22 years). The mean follow-up interval was 6 months, with a range of 3 to 15 months. Of the 152 probing sites measured, 92 (61%) decreased, 56 (37%) remained unchanged, and only 4 (2.6%) increased. A decrease in probing depth of 1 mm was found in 35 (23%) of the sites, a decrease of 2 mm was seen in 32 (21%), and 25 (16%) decreased by 3 mm or more. Of the 152 sites probed, 4 (2.6%) increased by 1 or 2 mm. Extraction of the impacted maxillary third molar does not result in significant periodontal defects on the distal aspect of the adjacent second molar, and in many cases it results in an improvement of the probing depths on these teeth. Copyright © 2011 American Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.

Key Challenges for Life Science Payloads on the Deep Space Gateway

NASA Astrophysics Data System (ADS)

Anthony, J. H.; Niederwieser, T.; Zea, L.; Stodieck, L.

2018-02-01

Compared to ISS, Deep Space Gateway life science payloads will be challenged by deep space radiation and non-continuous habitation. The impacts of these two differences on payload requirements, design, and operations are discussed.

Projected pH reductions by 2100 might put deep North Atlantic biodiversity at risk

NASA Astrophysics Data System (ADS)

Gehlen, M.; Séférian, R.; Jones, D. O. B.; Roy, T.; Roth, R.; Barry, J.; Bopp, L.; Doney, S. C.; Dunne, J. P.; Heinze, C.; Joos, F.; Orr, J. C.; Resplandy, L.; Segschneider, J.; Tjiputra, J.

2014-12-01

This study aims to evaluate the potential for impacts of ocean acidification on North Atlantic deep-sea ecosystems in response to IPCC AR5 Representative Concentration Pathways (RCPs). Deep-sea biota is likely highly vulnerable to changes in seawater chemistry and sensitive to moderate excursions in pH. Here we show, from seven fully coupled Earth system models, that for three out of four RCPs over 17% of the seafloor area below 500 m depth in the North Atlantic sector will experience pH reductions exceeding -0.2 units by 2100. Increased stratification in response to climate change partially alleviates the impact of ocean acidification on deep benthic environments. We report on major pH reductions over the deep North Atlantic seafloor (depth >500 m) and at important deep-sea features, such as seamounts and canyons. By 2100, and under the high CO2 scenario RCP8.5, pH reductions exceeding -0.2 (-0.3) units are projected in close to 23% (~15%) of North Atlantic deep-sea canyons and ~8% (3%) of seamounts - including seamounts proposed as sites of marine protected areas. The spatial pattern of impacts reflects the depth of the pH perturbation and does not scale linearly with atmospheric CO2 concentration. Impacts may cause negative changes of the same magnitude or exceeding the current target of 10% of preservation of marine biomes set by the convention on biological diversity, implying that ocean acidification may offset benefits from conservation/management strategies relying on the regulation of resource exploitation.

IMPACT OF POLYCYCLIC AROMATIC HYDROCARBONS OF THE ELECTROCHEMICAL RESPONSES OF A FERRICYNIDE PROBE AT TEMPLATE-MODIFIED SELF ASSEMBLED MONOLAYERS ON GOLD ELECTRODES

EPA Science Inventory

The impact of pyrene on the electrochemical response of the ferricyanide probe using Self Assembled Monolayer (SAM)-modified gold electrodes was investigated using Cyclic Voltammetry (CV) and Square Wave Voltammetry (SWV). These results suggest the feasibility of using SAMs, par...

49 CFR 572.72 - Head assembly and test procedure.

Code of Federal Regulations, 2010 CFR

2010-10-01

... intersection of the head midsagittal plane and the transverse plane which is perpendicular to the Z axis of the... and midsagittal planes passing through this point. (3) Impact the head with the test probe so that at... in the dummy's midsagittal plane. (4) Guide the test probe during impact so that there is no...

Distinguishing between relaxation pathways by combining dissociative ionization pump probe spectroscopy and ab initio calculations: a case study of cytosine.

PubMed

Kotur, Marija; Weinacht, Thomas C; Zhou, Congyi; Kistler, Kurt A; Matsika, Spiridoula

2011-05-14

We present a general method for tracking molecular relaxation along different pathways from an excited state down to the ground state. We follow the excited state dynamics of cytosine pumped near the S(0)-S(1) resonance using ultrafast laser pulses in the deep ultraviolet and probed with strong field near infrared pulses which ionize and dissociate the molecules. The fragment ions are detected via time of flight mass spectroscopy as a function of pump probe delay and probe pulse intensity. Our measurements reveal that different molecular fragments show different timescales, indicating that there are multiple relaxation pathways down to the ground state. We interpret our measurements with the help of ab initio electronic structure calculations of both the neutral molecule and the molecular cation for different conformations en route to relaxation back down to the ground state. Our measurements and calculations show passage through two seams of conical intersections between ground and excited states and demonstrate the ability of dissociative ionization pump probe measurements in conjunction with ab initio electronic structure calculations to track molecular relaxation through multiple pathways.

Population structure of microbial communities associated with two deep, anaerobic, alkaline aquifers.

PubMed Central

Fry, N K; Fredrickson, J K; Fishbain, S; Wagner, M; Stahl, D A

1997-01-01

Microbial communities of two deep (1,270 and 316 m) alkaline (pH 9.94 and 8.05), anaerobic (Eh, -137 and -27 mV) aquifers were characterized by rRNA-based analyses. Both aquifers, the Grande Ronde (GR) and Priest rapids (PR) formations, are located within the Columbia River Basalt Group in south-central Washington, and sulfidogenesis and methanogenesis characterize the GR and PR formations, respectively. RNA was extracted from microorganisms collected from groundwater by ultrafiltration through hollow-fiber membranes and hybridized to taxon-specific oligonucleotide probes. Of the three domains, Bacteria dominated both communities, making up to 92.0 and 64.4% of the total rRNA from the GR and PR formations, respectively. Eucarya comprised 5.7 and 14.4%, and Archaea comprised 1.8% and 2.5%, respectively. The gram-positive target group was found in both aquifers, 11.7% in GR and 7.6% in PR. Two probes were used to target sulfate- and/or metal-reducing bacteria within the delta subclass of Proteobacteria. The Desulfobacter groups was present (0.3%) only in the high-sulfate groundwater (GR). However, comparable hybridization to a probe selective for the desulfovibrios and some metal-reducing bacteria was found in both aquifers, 2.5 and 2.9% from the GR and PR formations, respectively. Selective PCR amplification and sequencing of the desulfovibrio/metal-reducing group revealed a predominance of desulfovibrios in both systems (17 of 20 clones), suggesting that their environmental distribution is not restricted by sulfate availability. PMID:9097447

Population structure of microbial communities associated with two deep, anaerobic, alkaline aquifers.

PubMed

Fry, N K; Fredrickson, J K; Fishbain, S; Wagner, M; Stahl, D A

1997-04-01

Microbial communities of two deep (1,270 and 316 m) alkaline (pH 9.94 and 8.05), anaerobic (Eh, -137 and -27 mV) aquifers were characterized by rRNA-based analyses. Both aquifers, the Grande Ronde (GR) and Priest rapids (PR) formations, are located within the Columbia River Basalt Group in south-central Washington, and sulfidogenesis and methanogenesis characterize the GR and PR formations, respectively. RNA was extracted from microorganisms collected from groundwater by ultrafiltration through hollow-fiber membranes and hybridized to taxon-specific oligonucleotide probes. Of the three domains, Bacteria dominated both communities, making up to 92.0 and 64.4% of the total rRNA from the GR and PR formations, respectively. Eucarya comprised 5.7 and 14.4%, and Archaea comprised 1.8% and 2.5%, respectively. The gram-positive target group was found in both aquifers, 11.7% in GR and 7.6% in PR. Two probes were used to target sulfate- and/or metal-reducing bacteria within the delta subclass of Proteobacteria. The Desulfobacter groups was present (0.3%) only in the high-sulfate groundwater (GR). However, comparable hybridization to a probe selective for the desulfovibrios and some metal-reducing bacteria was found in both aquifers, 2.5 and 2.9% from the GR and PR formations, respectively. Selective PCR amplification and sequencing of the desulfovibrio/metal-reducing group revealed a predominance of desulfovibrios in both systems (17 of 20 clones), suggesting that their environmental distribution is not restricted by sulfate availability.

Extreme Environment Technologies for Space and Terrestrial Applications

NASA Technical Reports Server (NTRS)

Balint, Tibor S.; Cutts, James A.; Kolawa, Elizabeth A.; Peterson, Craig E.

2008-01-01

Over the next decades, NASA's planned solar system exploration missions are targeting planets, moons and small bodies, where spacecraft would be expected to encounter diverse extreme environmental (EE) conditions throughout their mission phases. These EE conditions are often coupled. For instance, near the surface of Venus and in the deep atmospheres of giant planets, probes would experience high temperatures and pressures. In the Jovian system low temperatures are coupled with high radiation. Other environments include thermal cycling, and corrosion. Mission operations could also introduce extreme conditions, due to atmospheric entry heat flux and deceleration. Some of these EE conditions are not unique to space missions; they can be encountered by terrestrial assets from the fields of defense,oil and gas, aerospace, and automotive industries. In this paper we outline the findings of NASA's Extreme Environments Study Team, including discussions on state of the art and emerging capabilities related to environmental protection, tolerance and operations in EEs. We will also highlight cross cutting EE mitigation technologies, for example, between high g-load tolerant impactors for Europa and instrumented projectiles on Earth; high temperature electronics sensors on Jupiter deep probes and sensors inside jet engines; and pressure vessel technologies for Venus probes and sea bottom monitors. We will argue that synergistic development programs between these fields could be highly beneficial and cost effective for the various agencies and industries. Some of these environments, however, are specific to space and thus the related technology developments should be spear headed by NASA with collaboration from industry and academia.

Shooting for Excellence: African American and Youth Culture in New Century Schools.

ERIC Educational Resources Information Center

Mahiri, Jabari

Two African American teachers teach English in the same inner-city high school. One teacher is successful--her students read, interact, and strive for success. The other teacher's students are frequently disruptive or are asleep. This book probes deep into the causes of classroom success and failure, as well as other issues that affect American…

Comparison of four lasers (λ = 650, 808, 980, and 1075 nm) for noninvasive creation of deep subsurface lesions in tissue

NASA Astrophysics Data System (ADS)

Chang, Chun-Hung; Wilson, Christopher R.; Fried, Nathaniel M.

2015-07-01

Lasers have been used in combination with applied cooling methods to preserve superficial skin layers (100's μm's) during cosmetic surgery. Preservation of a thicker tissue surface layer (millimeters) may also allow development of other noninvasive laser procedures. We are exploring noninvasive therapeutic laser applications in urology (e.g. laser vasectomy and laser treatment of female stress urinary incontinence), which require surface tissue preservation on the millimeter scale. In this preliminary study, four lasers were compared for noninvasive creation of deep subsurface thermal lesions. Laser energy from three diode lasers (650, 808, and 980 nm) and a Ytterbium fiber laser (1075 nm) was delivered through a custom built, side-firing, laser probe with integrated cooling. An alcohol-based solution at -5 °C was circulated through a flow cell, cooling a sapphire window, which in turn cooled the tissue surface. The probe was placed in contact with porcine liver tissue, ex vivo, kept hydrated in saline and maintained at ~ 35 °C. Incident laser power was 4.2 W, spot diameter was 5.3 mm, and treatment time was 60 s. The optimal laser wavelength tested for creation of deep subsurface thermal lesions during contact cooling of tissues was 1075 nm, which preserved a surface layer of ~ 2 mm. The Ytterbium fiber laser provides a compact, low maintenance, and high power alternative laser source to the Neodymium:YAG laser for noninvasive thermal therapy.

Pulsed Laser Techniques to Determine Lattice and Radiative Thermal Conductivity of Deep Planetary Materials at Extreme Pressure-Temperature Conditions

NASA Astrophysics Data System (ADS)

Lobanov, S.; Goncharov, A. F.; Holtgrewe, N.; Konopkova, Z.; McWilliams, R. S.

2017-12-01

Thermal conductivity of deep planetary materials determines the planetary heat transport mode and properties (e.g. magnetic field) and can be used to decipher the planetary thermal history. Due to the lack of direct measurements of the lattice and radiative conductivity of the relevant materials at the planetary conditions, the current geodynamical models use theoretical calculations and extrapolations of the available experimental data. Here we describe our pulsed laser techniques that enable direct measurements of the lattice and radiative lattice conductivity of the Earth's mantle and core materials and also of noble gases and simple molecules present in the interiors of giant planets (e.g. hydrogen). Flash heating laser techniques working in a pump-probe mode that include time resolved two-side radiative and thermoreflection temperature probes employ various laser and photo-detector configurations, which provide a measure of the thermal fluxes propagating through the samples confined in the diamond anvil cell cavity. A supercontinuum ultra-bright broadband laser source empower accurate measurements of the optical properties of planetary materials used to extract the radiative conductivity. Finite element calculations serve to extract the temperature and pressure dependent thermal conductivity and temperature gradients across the sample. We report thermal conductivity measurements of the Earth's minerals (postperovskite, bridgmanite, ferropericlase) and their assemblies (pyrolite) and core materials (Fe and alloys with Si and O) at the realistic deep Earth's pressure temperature conditions. We thank J.-F.Lin, M. Murakami, J. Badro for contributing to this work.

Acoustic sand detector for fluid flowstreams

DOEpatents

Beattie, Alan G.; Bohon, W. Mark

1993-01-01

The particle volume and particle mass production rate of particulate solids entrained in fluid flowstreams such as formation sand or fracture proppant entrained in oil and gas production flowstreams is determined by a system having a metal probe interposed in a flow conduit for transmitting acoustic emissions created by particles impacting the probe to a sensor and signal processing circuit which produces discrete signals related to the impact of each of the particles striking the probe. The volume or mass flow rate of particulates is determined from making an initial particle size distribution and particle energy distribution and comparing the initial energy distribution and/or the initial size distribution with values related to the impact energies of a predetermined number of recorded impacts. The comparison is also used to recalibrate the system to compensate for changes in flow velocity.

Periodontal probing of an impacted tooth recovered through a surgical-orthodontic approach: a case report.

PubMed

Dinoi, Maria Teresa; Lacarbonara, Mariano; Dimartino, Salvatore; Monaco, Annalisa; Marzo, Giuseppe

2014-01-27

The aim of this work was to assess the periodontal support of a central upper incisor recovered through a surgical-orthodontic approach compared to the spontaneously erupted contralateral incisor. This case study describes an 8-year-old Caucasian female with an impacted upper right central incisor. Surgical-orthodontic treatment was performed to reset the impacted dental element in the arch. Periodontal probing was performed of all sites (mesio-buccal, central-buccal, disto-buccal, mesio-palatal, central-palatal and disto-palatal) of the recovered impacted tooth and the contralateral tooth. The results were compared to determine whether the treated element showed signs of periodontal injury. Most of the probing results on both her right and left incisors gave values of approximately 3mm, which were not considered pathological. Both dental elements had adequate and physiological osseous attachments.

Temperature and density evolution during decay in a 2.45 GHz hydrogen electron cyclotron resonance plasma: off-resonant and resonant cases.

PubMed

Cortázar, O D; Megía-Macías, A; Vizcaíno-de-Julián, A

2013-09-01

Time resolved electron temperature and density measurements during the decay stage in a hydrogen electron cyclotron resonance (ECR) plasma are presented for a resonance and off-resonance magnetic field configurations. The measurements are conducted on a ECR plasma generator excited at 2.45 GHz denominated test-bench for ion-sources plasma studies at ESS Bilbao. The plasma parameters evolution is studied by Langmuir probe diagnostic with synchronized sample technique developed for repetitive pulsed plasmas with a temporal resolution of 200 ns in typical decay processes of about 40 μs. An afterglow transient is clearly observed in the reflected microwave power signal from the plasma. Simultaneously, the electron temperature evolution shows rebounding peaks that may be related to the interplay between density drop and microwave coupling with deep impact on the Electron Energy Distribution Function. The correlation of such structures with the plasma absorbed power and the coupling quality is also reported.

Automated cell-type classification in intact tissues by single-cell molecular profiling

PubMed Central

2018-01-01

A major challenge in biology is identifying distinct cell classes and mapping their interactions in vivo. Tissue-dissociative technologies enable deep single cell molecular profiling but do not provide spatial information. We developed a proximity ligation in situ hybridization technology (PLISH) with exceptional signal strength, specificity, and sensitivity in tissue. Multiplexed data sets can be acquired using barcoded probes and rapid label-image-erase cycles, with automated calculation of single cell profiles, enabling clustering and anatomical re-mapping of cells. We apply PLISH to expression profile ~2900 cells in intact mouse lung, which identifies and localizes known cell types, including rare ones. Unsupervised classification of the cells indicates differential expression of ‘housekeeping’ genes between cell types, and re-mapping of two sub-classes of Club cells highlights their segregated spatial domains in terminal airways. By enabling single cell profiling of various RNA species in situ, PLISH can impact many areas of basic and medical research. PMID:29319504

The Giotto radio-science experiment

NASA Technical Reports Server (NTRS)

Edenhofer, P.; Bird, M. K.; Buschert, H.; Esposito, P. B.; Porsche, H.; Volland, H.

1986-01-01

The scientific objectives of the Giotto Radio Science Experiment (GRE) are to determine the columnar electron content of Comet Halley/s ionosphere and the cometary mass fluence from atmospheric drag by using the radio signals from Giotto during the Halley encounter. The radio science data (S and X-band Doppler and range measurements) will be collected at NASA/s deep-space 64 m tracking antenna at Tidbinbilla near Canberra, in Australia. In order to separate the effects of the terrestrial ionosphere and the interplanetary plasma, S-band Doppler measurements will also be taken at Tidbinbilla along the line-of-sight of Japan/s cometary probe Sakigake during the Giotto-Halley Encounter. The measurements of cometary electron content and mass fluence will be inverted to derive the spatial distribution of the electron and mass (dust and gas) density within Halley/s coma. The GRE is the only experiment on Giotto capable of measuring the low-energy (10 eV) electron bulk population of Halley/s ionosphere and the total cometary mass flow impacting upon the spacecraft.

Art By Don Davis Artist's concept of one of the probes on the hot surface of Venus. Although the

NASA Technical Reports Server (NTRS)

1977-01-01

Art By Don Davis Artist's concept of one of the probes on the hot surface of Venus. Although the probes were not designed to withstand impact, there was a chance that one might survive and transmit some data from the surface. A small probe did survive and transmitted data for 67 minutes.

KSC-05pp0138

NASA Image and Video Library

2005-01-12

KENNEDY SPACE CENTER, FLA. - Emerging through the smoke and steam, the Boeing Delta II rocket carrying NASA’s Deep Impact spacecraft lifts off at 1:47 p.m. EST from Launch Pad 17-B, Cape Canaveral Air Force Station, Fla. A NASA Discovery mission, Deep Impact is heading for space and a rendezvous 83 million miles from Earth with Comet Tempel 1. After releasing a 3- by 3-foot projectile (impactor) to crash onto the surface July 4, 2005, Deep Impact’s flyby spacecraft will reveal the secrets of the comet’s interior by collecting pictures and data of how the crater forms, measuring the crater’s depth and diameter as well as the composition of the interior of the crater and any material thrown out, and determining the changes in natural outgassing produced by the impact. It will send the data back to Earth through the antennas of the Deep Space Network.

Estimation of a melting probe's penetration velocity range to reach icy moons' subsurface ocean

NASA Astrophysics Data System (ADS)

Erokhina, Olga; Chumachenko, Eugene

2014-05-01

In modern space science one of the actual branches is icy satellites explorations. The main interest is concentrated around Jovian's moons Europa and Ganymede, Saturn's moons Titan and Enceladus that are covered by thick icy layer according to "Voyager1", "Voyager2", "Galileo" and "Cassini" missions. There is a big possibility that under icy shell could be a deep ocean. Also conditions on these satellites allow speculating about possible habitability, and considering these moons from an astrobiological point of view. One of the possible tasks of planned missions is a subsurface study. For this goal it is necessary to design special equipment that could be suitable for planetary application. One of the possible means is to use a melting probe which operates by melting and moves by gravitational force. Such a probe should be relatively small, should not weight too much and should require not too much energy. In terrestrial case such kind of probe has been successfully used for glaciers study. And it is possible to extrapolate the usage of such probe to extraterrestrial application. One of the tasks is to estimate melting probe's penetration velocity. Although there are other unsolved problems such as analyzing how the probe will move in low gravity and low atmospheric pressure; knowing whether hole will be closed or not when probe penetrate thick enough; and considering what order could be a penetration velocity. This study explores two techniques of melting probe's movement. One of them based on elasto-plastic theory and so-called "solid water" theory, and other one takes phase changing into account. These two techniques allow estimating melting probe's velocity range and study whole process. Based on these technique several cases of melting probe movement were considered, melting probe's velocity range estimated, influence of different factors studied and discussed and an easy way to optimize parameters of the melting probe proposed.

Galileo Probe Doppler Residuals as the Wave-Dynamical Signature of Weakly Stable, Downward-Increasing Stratification in Jupiter's Deep Wind Layer

NASA Technical Reports Server (NTRS)

Allison, Michael; Atkinson, David H.; Hansen, James E. (Technical Monitor)

2001-01-01

Doppler radio tracking of the Galileo probe-to-orbiter relay, previously analyzed for its in situ measure of Jupiter's zonal wind at the equatorial entry site, also shows a record of significant residual fluctuations apparently indicative of varying vertical motions. Regular oscillations over pressure depth in the residual Doppler measurements of roughly 1-8 Hz (increasing upward), as filtered over a 134 sec window, are most plausibly interpreted as gravity waves, and imply a weak, but downward increasing static stability within the 5 - 20 bar region of Jupiter's atmosphere. A matched extension to deeper levels of an independent inertial stability constraint from the measured vertical wind shear at 1 - 4 bars is roughly consistent with a static stability of approximately 0.5 K/km near the 20 bar level, as independently detected by the probe Atmospheric Structure Instrument.

Laminar recordings in frontal cortex suggest distinct layers for maintenance and control of working memory

PubMed Central

Bastos, André M.; Loonis, Roman; Kornblith, Simon; Lundqvist, Mikael; Miller, Earl K.

2018-01-01

All of the cerebral cortex has some degree of laminar organization. These different layers are composed of neurons with distinct connectivity patterns, embryonic origins, and molecular profiles. There are little data on the laminar specificity of cognitive functions in the frontal cortex, however. We recorded neuronal spiking/local field potentials (LFPs) using laminar probes in the frontal cortex (PMd, 8A, 8B, SMA/ACC, DLPFC, and VLPFC) of monkeys performing working memory (WM) tasks. LFP power in the gamma band (50–250 Hz) was strongest in superficial layers, and LFP power in the alpha/beta band (4–22 Hz) was strongest in deep layers. Memory delay activity, including spiking and stimulus-specific gamma bursting, was predominately in superficial layers. LFPs from superficial and deep layers were synchronized in the alpha/beta bands. This was primarily unidirectional, with alpha/beta bands in deep layers driving superficial layer activity. The phase of deep layer alpha/beta modulated superficial gamma bursting associated with WM encoding. Thus, alpha/beta rhythms in deep layers may regulate the superficial layer gamma bands and hence maintenance of the contents of WM. PMID:29339471

Computational analysis of transcranial magnetic stimulation in the presence of deep brain stimulation probes

NASA Astrophysics Data System (ADS)

Syeda, F.; Holloway, K.; El-Gendy, A. A.; Hadimani, R. L.

2017-05-01

Transcranial Magnetic Stimulation is an emerging non-invasive treatment for depression, Parkinson's disease, and a variety of other neurological disorders. Many Parkinson's patients receive the treatment known as Deep Brain Stimulation, but often require additional therapy for speech and swallowing impairment. Transcranial Magnetic Stimulation has been explored as a possible treatment by stimulating the mouth motor area of the brain. We have calculated induced electric field, magnetic field, and temperature distributions in the brain using finite element analysis and anatomically realistic heterogeneous head models fitted with Deep Brain Stimulation leads. A Figure of 8 coil, current of 5000 A, and frequency of 2.5 kHz are used as simulation parameters. Results suggest that Deep Brain Stimulation leads cause surrounding tissues to experience slightly increased E-field (Δ Emax =30 V/m), but not exceeding the nominal values induced in brain tissue by Transcranial Magnetic Stimulation without leads (215 V/m). The maximum temperature in the brain tissues surrounding leads did not change significantly from the normal human body temperature of 37 °C. Therefore, we ascertain that Transcranial Magnetic Stimulation in the mouth motor area may stimulate brain tissue surrounding Deep Brain Stimulation leads, but will not cause tissue damage.

Laminar recordings in frontal cortex suggest distinct layers for maintenance and control of working memory.

PubMed

Bastos, André M; Loonis, Roman; Kornblith, Simon; Lundqvist, Mikael; Miller, Earl K

2018-01-30

All of the cerebral cortex has some degree of laminar organization. These different layers are composed of neurons with distinct connectivity patterns, embryonic origins, and molecular profiles. There are little data on the laminar specificity of cognitive functions in the frontal cortex, however. We recorded neuronal spiking/local field potentials (LFPs) using laminar probes in the frontal cortex (PMd, 8A, 8B, SMA/ACC, DLPFC, and VLPFC) of monkeys performing working memory (WM) tasks. LFP power in the gamma band (50-250 Hz) was strongest in superficial layers, and LFP power in the alpha/beta band (4-22 Hz) was strongest in deep layers. Memory delay activity, including spiking and stimulus-specific gamma bursting, was predominately in superficial layers. LFPs from superficial and deep layers were synchronized in the alpha/beta bands. This was primarily unidirectional, with alpha/beta bands in deep layers driving superficial layer activity. The phase of deep layer alpha/beta modulated superficial gamma bursting associated with WM encoding. Thus, alpha/beta rhythms in deep layers may regulate the superficial layer gamma bands and hence maintenance of the contents of WM. Copyright © 2018 the Author(s). Published by PNAS.

DC3 Data and Information Page

Atmospheric Science Data Center

2015-03-16

Deep Convective Clouds and Chemistry (DC3) Data and Information The Deep Convective Clouds and Chemistry ( DC3 ) field campaign is investigating the impact of deep, ... processes, on upper tropospheric (UT) composition and chemistry. The primary science objectives are:   To quantify and ...

Projected pH reductions by 2100 might put deep North Atlantic biodiversity at risk

NASA Astrophysics Data System (ADS)

Gehlen, M.; Séférian, R.; Jones, D. O. B.; Roy, T.; Roth, R.; Barry, J.; Bopp, L.; Doney, S. C.; Dunne, J. P.; Heinze, C.; Joos, F.; Orr, J. C.; Resplandy, L.; Segschneider, J.; Tjiputra, J.

2014-06-01

This study aims at evaluating the potential for impacts of ocean acidification on North Atlantic deep-sea ecosystems in response to IPCC AR5 Representative Concentration Pathways (RCP). Deep-sea biota is likely highly vulnerable to changes in seawater chemistry and sensitive to moderate excursions in pH. Here we show, from seven fully-coupled Earth system models, that for three out of four RCPs over 17% of the seafloor area below 500 m depth in the North Atlantic sector will experience pH reductions exceeding -0.2 units by 2100. Increased stratification in response to climate change partially alleviates the impact of ocean acidification on deep benthic environment. We report major potential consequences of pH reductions for deep-sea biodiversity hotspots, such as seamounts and canyons. By 2100 and under the high CO2 scenario RCP8.5 pH reductions exceeding -0.2, (respectively -0.3) units are projected in close to 23% (~ 15%) of North Atlantic deep-sea canyons and ~ 8% (3%) of seamounts - including seamounts proposed as sites of marine protected areas. The spatial pattern of impacts reflects the depth of the pH perturbation and does not scale linearly with atmospheric CO2 concentration. Impacts may cause negative changes of the same magnitude or exceeding the current target of 10% of preservation of marine biomes set by the convention on biological diversity implying that ocean acidification may offset benefits from conservation/management strategies relying on the regulation of resource exploitation.

Deep Impact Sequence Planning Using Multi-Mission Adaptable Planning Tools With Integrated Spacecraft Models

NASA Technical Reports Server (NTRS)

Wissler, Steven S.; Maldague, Pierre; Rocca, Jennifer; Seybold, Calina

2006-01-01

The Deep Impact mission was ambitious and challenging. JPL's well proven, easily adaptable multi-mission sequence planning tools combined with integrated spacecraft subsystem models enabled a small operations team to develop, validate, and execute extremely complex sequence-based activities within very short development times. This paper focuses on the core planning tool used in the mission, APGEN. It shows how the multi-mission design and adaptability of APGEN made it possible to model spacecraft subsystems as well as ground assets throughout the lifecycle of the Deep Impact project, starting with models of initial, high-level mission objectives, and culminating in detailed predictions of spacecraft behavior during mission-critical activities.

Lyman-alpha fractions in the Hubble Ultra Deep Field at 4 < z < 6

NASA Astrophysics Data System (ADS)

Harish, Santosh; Malhotra, Sangeeta; Rhoads, James; Christensen, Lise; Tilvi, Vithal; Finkelstein, Steven; Pharo, John

2018-01-01

Lyman-alpha (Lya) emitting galaxies at high-redshifts serve as a good probe of neutral hydrogen in the intergalactic medium (IGM). Here we present measurements of the Lya fraction using a sample of Lyman-break galaxies (LBGs) between 4 < z < 6 with deep HST grism observations from the GRAPES/PEARS projects as well as spectroscopic observations from the MUSE integral-field spectrograph. The sample of LBGs at z~5 & 6 are spectroscopically confirmed with deep HST grism data from the GRAPES and PEARS projects. We also measure Lya fractions using a sample of photometrically-selected LBGs for the same redshift range. In addition, we study the EW distribution in relation to continuum and line luminosities, as well as the relation between photometric and spectroscopic redshift. We find that objects with higher EWs tend to have larger differences between photometric and spectroscopic redshifts.

Attenuation of deep semantic processing during mind wandering: an event-related potential study.

PubMed

Xu, Judy; Friedman, David; Metcalfe, Janet

2018-03-21

Although much research shows that early sensory and attentional processing is affected by mind wandering, the effect of mind wandering on deep (i.e. semantic) processing is relatively unexplored. To investigate this relation, we recorded event-related potentials as participants studied English-Spanish word pairs, one at a time, while being intermittently probed for whether they were 'on task' or 'mind wandering'. Both perceptual processing, indexed by the P2 component, and deep processing, indexed by a late, sustained slow wave maximal at parietal electrodes, was attenuated during periods preceding participants' mind wandering reports. The pattern when participants were on task, rather than mind wandering, is similar to the subsequent memory or difference in memory effect. These results support previous findings of sensory attenuation during mind wandering, and extend them to a long-duration slow wave by suggesting that the deeper and more sustained levels of processing are also disrupted.

The promise and peril of chemical probes

PubMed Central

Arrowsmith, Cheryl H; Audia, James E; Austin, Christopher; Baell, Jonathan; Bennett, Jonathan; Blagg, Julian; Bountra, Chas; Brennan, Paul E; Brown, Peter J; Bunnage, Mark E; Buser-Doepner, Carolyn; Campbell, Robert M; Carter, Adrian J; Cohen, Philip; Copeland, Robert A; Cravatt, Ben; Dahlin, Jayme L; Dhanak, Dashyant; Frederiksen, Mathias; Frye, Stephen V; Gray, Nathanael; Grimshaw, Charles E; Hepworth, David; Howe, Trevor; Huber, Kilian V M; Jin, Jian; Knapp, Stefan; Kotz, Joanne D; Kruger, Ryan G; Lowe, Derek; Mader, Mary M; Marsden, Brian; Mueller-Fahrnow, Anke; Müller, Susanne; O'Hagan, Ronan C; Overington, John P; Owen, Dafydd R; Rosenberg, Saul H; Ross, Ruth; Roth, Bryan; Schapira, Matthieu; Schreiber, Stuart L; Shoichet, Brian; Sundström, Michael; Superti-Furga, Giulio; Taunton, Jack; Toledo-Sherman, Leticia; Walpole, Chris; Walters, Michael A; Willson, Timothy M; Workman, Paul; Young, Robert N; Zuercher, William J

2016-01-01

Chemical probes are powerful reagents with increasing impacts on biomedical research. However, probes of poor quality or that are used incorrectly generate misleading results. To help address these shortcomings, we will create a community-driven wiki resource to improve quality and convey current best practice. PMID:26196764

Plastic microfibre ingestion by deep-sea organisms

NASA Astrophysics Data System (ADS)

Taylor, M. L.; Gwinnett, C.; Robinson, L. F.; Woodall, L. C.

2016-09-01

Plastic waste is a distinctive indicator of the world-wide impact of anthropogenic activities. Both macro- and micro-plastics are found in the ocean, but as yet little is known about their ultimate fate and their impact on marine ecosystems. In this study we present the first evidence that microplastics are already becoming integrated into deep-water organisms. By examining organisms that live on the deep-sea floor we show that plastic microfibres are ingested and internalised by members of at least three major phyla with different feeding mechanisms. These results demonstrate that, despite its remote location, the deep sea and its fragile habitats are already being exposed to human waste to the extent that diverse organisms are ingesting microplastics.

Impact disruption and recovery of the deep subsurface biosphere

USGS Publications Warehouse

Cockell, Charles S.; Voytek, Mary A.; Gronstal, Aaron L.; Finster, Kai; Kirshtein, Julie D.; Howard, Kieren; Reitner, Joachim; Gohn, Gregory S.; Sanford, Ward E.; Horton, J. Wright; Kallmeyer, Jens; Kelly, Laura; Powars, David S.

2012-01-01

Although a large fraction of the world's biomass resides in the subsurface, there has been no study of the effects of catastrophic disturbance on the deep biosphere and the rate of its subsequent recovery. We carried out an investigation of the microbiology of a 1.76 km drill core obtained from the ~35 million-year-old Chesapeake Bay impact structure, USA, with robust contamination control. Microbial enumerations displayed a logarithmic downward decline, but the different gradient, when compared to previously studied sites, and the scatter of the data are consistent with a microbiota influenced by the geological disturbances caused by the impact. Microbial abundance is low in buried crater-fill, ocean-resurge, and avalanche deposits despite the presence of redox couples for growth. Coupled with the low hydraulic conductivity, the data suggest the microbial community has not yet recovered from the impact ~35 million years ago. Microbial enumerations, molecular analysis of microbial enrichment cultures, and geochemical analysis showed recolonization of a deep region of impact-fractured rock that was heated to above the upper temperature limit for life at the time of impact. These results show how, by fracturing subsurface rocks, impacts can extend the depth of the biosphere. This phenomenon would have provided deep refugia for life on the more heavily bombarded early Earth, and it shows that the deeply fractured regions of impact craters are promising targets to study the past and present habitability of Mars.

Impact disruption and recovery of the deep subsurface biosphere.

PubMed

Cockell, Charles S; Voytek, Mary A; Gronstal, Aaron L; Finster, Kai; Kirshtein, Julie D; Howard, Kieren; Reitner, Joachim; Gohn, Gregory S; Sanford, Ward E; Horton, J Wright; Kallmeyer, Jens; Kelly, Laura; Powars, David S

2012-03-01

Although a large fraction of the world's biomass resides in the subsurface, there has been no study of the effects of catastrophic disturbance on the deep biosphere and the rate of its subsequent recovery. We carried out an investigation of the microbiology of a 1.76 km drill core obtained from the ∼35 million-year-old Chesapeake Bay impact structure, USA, with robust contamination control. Microbial enumerations displayed a logarithmic downward decline, but the different gradient, when compared to previously studied sites, and the scatter of the data are consistent with a microbiota influenced by the geological disturbances caused by the impact. Microbial abundance is low in buried crater-fill, ocean-resurge, and avalanche deposits despite the presence of redox couples for growth. Coupled with the low hydraulic conductivity, the data suggest the microbial community has not yet recovered from the impact ∼35 million years ago. Microbial enumerations, molecular analysis of microbial enrichment cultures, and geochemical analysis showed recolonization of a deep region of impact-fractured rock that was heated to above the upper temperature limit for life at the time of impact. These results show how, by fracturing subsurface rocks, impacts can extend the depth of the biosphere. This phenomenon would have provided deep refugia for life on the more heavily bombarded early Earth, and it shows that the deeply fractured regions of impact craters are promising targets to study the past and present habitability of Mars.

The design of the optical Thomson scattering diagnostic for the National Ignition Facility.

PubMed

Datte, P S; Ross, J S; Froula, D H; Daub, K D; Galbraith, J; Glenzer, S; Hatch, B; Katz, J; Kilkenny, J; Landen, O; Manha, D; Manuel, A M; Molander, W; Montgomery, D; Moody, J; Swadling, G F; Weaver, J

2016-11-01

The National Ignition Facility (NIF) is a 192 laser beam facility designed to support the Stockpile Stewardship, High Energy Density and Inertial Confinement Fusion (ICF) programs. We report on the design of an Optical Thomson Scattering (OTS) diagnostic that has the potential to transform the community's understanding of NIF hohlraum physics by providing first principle, local, time-resolved measurements of under-dense plasma conditions. The system design allows operation with different probe laser wavelengths by manual selection of the appropriate beam splitter and gratings before the shot. A deep-UV probe beam (λ 0 -210 nm) will be used to optimize the scattered signal for plasma densities of 5 × 10 20 electrons/cm 3 while a 3ω probe will be used for experiments investigating lower density plasmas of 1 × 10 19 electrons/cm 3 . We report the phase I design of a two phase design strategy. Phase I includes the OTS telescope, spectrometer, and streak camera; these will be used to assess the background levels at NIF. Phase II will include the design and installation of a probe laser.

Probing Water Ice for Trace Organics using Raman Scattering in the Laboratory and Terrestrial Field Analogs

NASA Astrophysics Data System (ADS)

Winebrenner, D. P.; Kirby, J. P.; Marquardt, B.

2013-12-01

Trace constituents in (predominantly) water ice are key to understanding planetary and astrobiological processes on a wide variety of solar system bodies, including Europa, Enceladus, Titan, Ceres, Mars, and comets. Organic traces are of particular interest not only for astrobiology but also, for example, for understanding the fates of abiotic organics delivered by meteorites. Raman scattering is known for specificity in identifying bonds and compounds, but has generally been considered relatively insensitive to concentrations characteristic of trace constituents. Here we test in doped water ice a Raman probe system that was developed for industrial and environmental applications and that has characterized select traces at ppm-levels near a deep-sea hydrothermal vent. We report in particular results for 17 amino acids and for aromatic hydrocarbons. Finally, based on physically robust Raman optics developed for industrial environments, we proposal a concept for subsurface investigation of traces in terrestrial analog environments such as glaciers and ice sheets, by means of integrating a Raman probe with a thermal ice melt probe recently field-tested in Greenland.

Lessons Learned from Daily Uplink Operations during the Deep Impact Mission

NASA Technical Reports Server (NTRS)

Stehly, Joseph S.

2006-01-01

The daily preparation of uplink products (commands and files) for Deep Impact was as problematic as the final encounter images were spectacular. The operations team was faced with many challenges during the six-month mission to comet Tempel One of the biggest difficulties was that the Deep Impact Flyby and Impactor vehicles necessitated a high volume of uplink products while also utilizing a new uplink file transfer capability. The Jet Propulsion Laboratory (JPL) Multi-Mission Ground Systems and Services (MGSS) Mission Planning and Sequence Team (MPST) had the responsibility of preparing the uplink products for use on the two spacecraft. These responsibilities included processing nearly 15,000 flight products, modeling the states of the spacecraft during all activities for subsystem review, and ensuring that the proper commands and files were uplinked to the spacecraft. To guarantee this transpired and the health and safety of the two spacecraft were not jeopardized several new ground scripts and procedures were developed while the Deep Impact Flyby and Impactor spacecraft were en route to their encounter with Tempel-1. These scripts underwent several adaptations throughout the entire mission up until three days before the separation of the Flyby and Impactor vehicles. The problems presented by Deep Impact's daily operations and the development of scripts and procedures to ease those challenges resulted in several valuable lessons learned. These lessons are now being integrated into the design of current and future MGSS missions at JPL.

Thiazole derivative-modified upconversion nanoparticles for Hg2+ detection in living cells

NASA Astrophysics Data System (ADS)

Gu, Bin; Zhou, Yi; Zhang, Xiao; Liu, Xiaowang; Zhang, Yuhai; Marks, Robert; Zhang, Hua; Liu, Xiaogang; Zhang, Qichun

2015-12-01

Mercury ion (Hg2+) is an extremely toxic ion, which will accumulate in human bodies and cause severe nervous system damage. Therefore, the sensitive and efficient monitoring of Hg2+ in human bodies is of great importance. Upconversion nanoparticle (UCNPs) based nano probes exhibit no autofluorescence, deep penetration depth and chemical stability in biological samples, as well as a large anti-stokes shift. In this study, we have developed thiazole-derivative-functionalized UCNPs, and employed an upconversion emission intensity ratio of 540 nm to 803 nm (I540/I803) as a ratiometric signal to detect Hg2+ in living cells showing excellent photo stability and high selectivity. Our nano probe was characterized using transmission electron microscopy (TEM) and powder X-ray diffraction (PXRD). The low cytotoxicity of our probe was confirmed by an MTT assay and the UCL test in HeLa cells was carried out by confocal microscopy. Our results demonstrated that organic-dye-functionalized UCNPs should be a good strategy for detecting toxic metal ions when studying cellular biosystems.Mercury ion (Hg2+) is an extremely toxic ion, which will accumulate in human bodies and cause severe nervous system damage. Therefore, the sensitive and efficient monitoring of Hg2+ in human bodies is of great importance. Upconversion nanoparticle (UCNPs) based nano probes exhibit no autofluorescence, deep penetration depth and chemical stability in biological samples, as well as a large anti-stokes shift. In this study, we have developed thiazole-derivative-functionalized UCNPs, and employed an upconversion emission intensity ratio of 540 nm to 803 nm (I540/I803) as a ratiometric signal to detect Hg2+ in living cells showing excellent photo stability and high selectivity. Our nano probe was characterized using transmission electron microscopy (TEM) and powder X-ray diffraction (PXRD). The low cytotoxicity of our probe was confirmed by an MTT assay and the UCL test in HeLa cells was carried out by confocal microscopy. Our results demonstrated that organic-dye-functionalized UCNPs should be a good strategy for detecting toxic metal ions when studying cellular biosystems. Electronic supplementary information (ESI) available: NMR, MALDI-TOF MS spectra, etc. See DOI: 10.1039/c5nr05286f

Ejecta from Ocean Impacts

NASA Technical Reports Server (NTRS)

Kyte, Frank T.

2003-01-01

Numerical simulations of deep-ocean impact provide some limits on the size of a projectile that will not mix with the ocean floor during a deep-ocean impact. For a vertical impact at asteroidal velocities (approx. 20 km/s), mixing is only likely when the projectile diameter is greater than 112 of the water depth. For oblique impacts, even larger projectiles will not mix with ocean floor silicates. Given the typical water depths of 4 to 5 km in deep-ocean basins, asteroidal projectiles with diameters as large as 2 or 3 km may commonly produce silicate ejecta that is composed only of meteoritic materials and seawater salts. However, the compressed water column beneath the projectile can still disrupt and shock metamorphose the ocean floor. Therefore, production of a separate, terrestrial ejecta component is not ruled out in the most extreme case. With increasing projectile size (or energy) relative to water depths, there must be a gradation between oceanic impacts and more conventional continental impacts. Given that 60% of the Earth's surface is covered by oceanic lithosphere and 500 m projectiles impact the Earth on 10(exp 5) y timescales, there must be hundreds of oceanic impact deposits in the sediment record awaiting discovery.

Radiation factors in space and a system for their monitoring.

PubMed

Kovtunenko, V M; Kremnev, R S; Pichkhadze, K M; Bogomolov, V B; Kontor, N N; Filippichev, S A; Petrov, V M; Pissarenko, N F

1994-10-01

The radiation environment is of special concern when the spaceship flies in deep space. The annual fluence of the galactic cosmic rays is approximately 10(8) cm-2 and the absorbed dose of the solar cosmic rays can reach 10 Gy per event behind the shielding thickness of 3-5 g cm-2 Al. For the radiation environment monitoring it is planned to place a measuring complex on the space probes "Mars" and "Spectr" flying outside the magnetosphere. This complex is to measure: cosmic rays composition, particle flux, dose equivalent, energy and LET spectra, solar X-rays spectrum. On line data transmission by the space probes permits to obtain the radiation environment data in space.

CryoScout: A Descent Through the Mars Polar Cap

NASA Technical Reports Server (NTRS)

Hecht, M. H.; Saunders, R. S.

2003-01-01

CryoScout was proposed as a subsurface investigation of the stratigraphic climate record embedded in Mars North Polar cap. After landing on a gentle landscape in the midst of the mild summer season, CryoScout was to use the continuous polar sunlight to power the descent of a cryobot, a thermal probe, into the ice at a rate of about 1 m per day. CryoScout would probe deep enough into this time capsule to see the effects of planetary obliquity variations and discrete events such as dust storms or volcanic eruptions. By penetrating tens of meters of ice, the mission would explore at least one of the dominant "MOC layers" observed in exposed layered terrain.

Ultrafast carrier dynamics in band edge and broad deep defect emission ZnSe nanowires

NASA Astrophysics Data System (ADS)

Othonos, Andreas; Lioudakis, Emmanouil; Philipose, U.; Ruda, Harry E.

2007-12-01

Ultrafast carrier dynamics of ZnSe nanowires grown under different growth conditions have been studied. Transient absorption measurements reveal the dependence of the competing effects of state filling and photoinduced absorption on the probed energy states. The relaxation of the photogenerated carriers occupying defect states in the stoichiometric and Se-rich samples are single exponentials with time constants of 3-4ps. State filling is the main contribution for probe energies below 1.85eV in the Zn-rich grown sample. This ultrafast carrier dynamics study provides an important insight into the role that intrinsic point defects play in the observed photoluminescence from ZnSe nanowires.

Very High Specific Energy, Medium Power Li/CFx Primary Battery for Launchers and Space Probes

NASA Astrophysics Data System (ADS)

Brochard, Paul; Godillot, Gerome; Peres, Jean Paul; Corbin, Julien; Espinosa, Amaya

2014-08-01

Benchmark with existing technologies shows the advantages of the lithium-fluorinated carbon (Li/CFx) technology for use aboard future launchers in terms of a low Total Cost of Ownership (TCO), especially for high energy demanding missions such as re-ignitable upper stages for long GTO+ missions and probes for deep space exploration.This paper presents the new results obtained on this chemistry in terms of electrical and climatic performances, abuse tests and life tests. Studies - co-financed between CNES and Saft - looked at a pure CFx version with a specific energy up to 500 Wh/kg along with a medium power of 80 to 100 W/kg.

Advances in deep-sea biology: biodiversity, ecosystem functioning and conservation. An introduction and overview

NASA Astrophysics Data System (ADS)

Cunha, Marina R.; Hilário, Ana; Santos, Ricardo S.

2017-03-01

Once considered as monotonous and devoid of life, the deep sea was revealed during the last century as an environment with a plethora of life forms and extremely high species richness (Rex and Etter, 2010). Underwater vehicle developments allowed direct observations of the deep, disclosing unique habitats and diverse seascapes, and other technological advances enabled manipulative experimentation and unprecedented prospects to pursue novel research topics (Levin and Sibuet, 2012; Danovaro et al., 2014). Alongside, the growing human population greatly increased the pressure on deep-sea ecosystems and the services they provide (Ramirez-Llodra et al., 2011; Thurber et al., 2014; Levin et al., 2016). Societal changes further intensified worldwide competition for natural resources, extending the present footprint of impacts over most of the global ocean (Halpern et al., 2008). In this socio-economic context, and in tandem with cutting edge technological advances and an unclear legal framework to regulate access to natural resources (Boyes and Elliott, 2014), the deep sea has emerged as a new opportunity for industrial exploitation and novel economic activities. The expanding use of the deep sea prompted a rapid reply from deep-sea scientists that recommended "a move from a frontier mentality of exploitation and single-sector management to a precautionary system that balances use of living marine resources, energy, and minerals from the deep ocean with maintenance of a productive and healthy marine environment, while improving knowledge and collaboration" and proposed "three directions to advance deep-ocean stewardship: i) protection and mitigation, ii) research, and iii) collaborative governance" (Mengerink et al., 2014). The European Marine Board position paper 22 (Rogers et al., 2015) further examined the key societal and environmental drivers confronting the deep sea and the role of deep-sea research to deliver future knowledge needs for science and society; a clear and consistent message from consultation with wider stakeholders was the need for fundamental knowledge of deep-sea ecosystems. Enhanced deep-sea knowledge is crucial to establish baselines and assess long term impact of human activity on ecosystems and it is also instrumental to inform environmental impact assessments, strategic management plans, effective decision making, environmental regulation and ocean governance (Rogers et al., 2015).

Development of a group-specific 16S rRNA-targeted probe set for the identification of Marinobacter by fluorescence in situ hybridization

NASA Astrophysics Data System (ADS)

McKay, Luke J.; Gutierrez, Tony; Teske, Andreas P.

2016-07-01

Members of the Marinobacter genus play an important role in hydrocarbon degradation in the ocean - a topic of special significance in light of the recent Deepwater Horizon oil spill of 2010. The Marinobacter group has thus far lacked a genus level phylogenetic probe that would allow in situ identification of representative members. Here, we developed two new 16S rRNA-targeted oligonucleotide probes (Mrb-0625-a and Mrb-0625-b) to enumerate Marinobacter species by fluorescence in situ hybridization (FISH). In silico analysis of this probe set demonstrated 80% coverage of the Marinobacter genus. A competitor probe was developed to block hybridization by Mrb-0625-a to six Halomonas species with which it shared a one base pair mismatch. The probe set was optimized using pure cultures, and then used in an enrichment experiment with a deep sea oil plume water sample collected from the Deepwater Horizon oil spill. Marinobacter cells rapidly increased as a significant fraction of total microbial abundance in all incubations of original contaminated seawater as well as those amended with n-hexadecane, suggesting this group may be among the first microbial responders to oil pollution in the marine environment. The new probe set will provide a reliable tool for quantifying Marinobacter in the marine environment, particularly at contaminated sites where these organisms can play an important role in the biodegradation of oil pollutants.

Impact of the Deepwater Horizon oil spill on a deep-water coral community in the Gulf of Mexico

PubMed Central

White, Helen K.; Hsing, Pen-Yuan; Cho, Walter; Shank, Timothy M.; Cordes, Erik E.; Quattrini, Andrea M.; Nelson, Robert K.; Camilli, Richard; Demopoulos, Amanda W. J.; German, Christopher R.; Brooks, James M.; Roberts, Harry H.; Shedd, William; Reddy, Christopher M.; Fisher, Charles R.

2012-01-01

To assess the potential impact of the Deepwater Horizon oil spill on offshore ecosystems, 11 sites hosting deep-water coral communities were examined 3 to 4 mo after the well was capped. Healthy coral communities were observed at all sites >20 km from the Macondo well, including seven sites previously visited in September 2009, where the corals and communities appeared unchanged. However, at one site 11 km southwest of the Macondo well, coral colonies presented widespread signs of stress, including varying degrees of tissue loss, sclerite enlargement, excess mucous production, bleached commensal ophiuroids, and covering by brown flocculent material (floc). On the basis of these criteria the level of impact to individual colonies was ranked from 0 (least impact) to 4 (greatest impact). Of the 43 corals imaged at that site, 46% exhibited evidence of impact on more than half of the colony, whereas nearly a quarter of all of the corals showed impact to >90% of the colony. Additionally, 53% of these corals’ ophiuroid associates displayed abnormal color and/or attachment posture. Analysis of hopanoid petroleum biomarkers isolated from the floc provides strong evidence that this material contained oil from the Macondo well. The presence of recently damaged and deceased corals beneath the path of a previously documented plume emanating from the Macondo well provides compelling evidence that the oil impacted deep-water ecosystems. Our findings underscore the unprecedented nature of the spill in terms of its magnitude, release at depth, and impact to deep-water ecosystems. PMID:22454495

Impact of the Deepwater Horizon oil spill on a deep-water coral community in the Gulf of Mexico.

PubMed

White, Helen K; Hsing, Pen-Yuan; Cho, Walter; Shank, Timothy M; Cordes, Erik E; Quattrini, Andrea M; Nelson, Robert K; Camilli, Richard; Demopoulos, Amanda W J; German, Christopher R; Brooks, James M; Roberts, Harry H; Shedd, William; Reddy, Christopher M; Fisher, Charles R

2012-12-11

To assess the potential impact of the Deepwater Horizon oil spill on offshore ecosystems, 11 sites hosting deep-water coral communities were examined 3 to 4 mo after the well was capped. Healthy coral communities were observed at all sites >20 km from the Macondo well, including seven sites previously visited in September 2009, where the corals and communities appeared unchanged. However, at one site 11 km southwest of the Macondo well, coral colonies presented widespread signs of stress, including varying degrees of tissue loss, sclerite enlargement, excess mucous production, bleached commensal ophiuroids, and covering by brown flocculent material (floc). On the basis of these criteria the level of impact to individual colonies was ranked from 0 (least impact) to 4 (greatest impact). Of the 43 corals imaged at that site, 46% exhibited evidence of impact on more than half of the colony, whereas nearly a quarter of all of the corals showed impact to >90% of the colony. Additionally, 53% of these corals' ophiuroid associates displayed abnormal color and/or attachment posture. Analysis of hopanoid petroleum biomarkers isolated from the floc provides strong evidence that this material contained oil from the Macondo well. The presence of recently damaged and deceased corals beneath the path of a previously documented plume emanating from the Macondo well provides compelling evidence that the oil impacted deep-water ecosystems. Our findings underscore the unprecedented nature of the spill in terms of its magnitude, release at depth, and impact to deep-water ecosystems.

Impact of the Deepwater Horizon oil spill on a deep-water coral community in the Gulf of Mexico

USGS Publications Warehouse

White, Helen K.; Hsing, Pen-Yuan; Cho, Walter; Shank, Timothy M.; Cordes, Erik E.; Quattrini, Andrea M.; Nelson, Robert K.; Camilli, Richard; Demopoulos, Amanda W.J.; German, Christopher R.; Brooks, James M.; Roberts, Harry H.; Shedd, William; Reddy, Christopher M.; Fisher, Charles R.

2012-01-01

To assess the potential impact of the Deepwater Horizon oil spill on offshore ecosystems, 11 sites hosting deep-water coral communities were examined 3 to 4 mo after the well was capped. Healthy coral communities were observed at all sites >20 km from the Macondo well, including seven sites previously visited in September 2009, where the corals and communities appeared unchanged. However, at one site 11 km southwest of the Macondo well, coral colonies presented widespread signs of stress, including varying degrees of tissue loss, sclerite enlargement, excess mucous production, bleached commensal ophiuroids, and covering by brown flocculent material (floc). On the basis of these criteria the level of impact to individual colonies was ranked from 0 (least impact) to 4 (greatest impact). Of the 43 corals imaged at that site, 46% exhibited evidence of impact on more than half of the colony, whereas nearly a quarter of all of the corals showed impact to >90% of the colony. Additionally, 53% of these corals’ ophiuroid associates displayed abnormal color and/or attachment posture. Analysis of hopanoid petroleum biomarkers isolated from the floc provides strong evidence that this material contained oil from the Macondo well. The presence of recently damaged and deceased corals beneath the path of a previously documented plume emanating from the Macondo well provides compelling evidence that the oil impacted deep-water ecosystems. Our findings underscore the unprecedented nature of the spill in terms of its magnitude, release at depth, and impact to deep-water ecosystems.

An Experiential Learning Approach to Teaching Social Entrepreneurship, Triple Bottom Line, and Sustainability: Modifying and Extending Practical Organizational Behavior Education (PROBE)

ERIC Educational Resources Information Center

Gundlach, Michael J.; Zivnuska, Suzanne

2010-01-01

When teaching social entrepreneurship and sustainability, using an experiential learning approach can be more effective than a traditional lecture approach. Social and environmental entrepreneurs often have a deep passion for their work that is important for students to develop early in their careers. Experiential learning enables students to…

Spitzer Lensing Cluster Legacy Survey

NASA Astrophysics Data System (ADS)

Soifer, Tom; Armus, Lee; Bradac, Marusa; Capak, Peter; Coe, Dan; Siana, Brian; Treu, Tommaso; Vieira, Joaquin

2015-11-01

Cluster-scale gravitational lenses act as cosmic telescopes, enabling the study of otherwise unobservable galaxies. They are critical in answering the questions such as what is the star formation history at z > 7, and whether these galaxies can reionize the Universe. Accurate knowledge of stellar masses, ages, and star formation rates at this epoch requires measuring both rest-frame UV and optical light, which only Spitzer and HST can probe at z>7-11 for a large enough sample of typical galaxies. To address this cosmic puzzle, we propose a program that obtains shallow Spitzer/IRAC imaging of a large sample of cluster lenses, followed by deep imaging of those clusters with the largest number of z > 7 candidate galaxies. This proposal will be a valuable Legacy complement to the existing IRAC deep surveys, and it will open up a new parameter space by probing the ordinary yet magnified population. Furthermore, it will enable the measurements of the stellar mass of the galaxy cluster population, thereby allowing us to chart the build-up of the cluster red sequence from z~1 to the present and to determine the physical processes responsible for this stellar mass growth.

Hand-held optoacoustic probe for three-dimensional imaging of human morphology and function

NASA Astrophysics Data System (ADS)

Deán-Ben, X. Luís.; Razansky, Daniel

2014-03-01

We report on a hand-held imaging probe for real-time optoacoustic visualization of deep tissues in three dimensions. The proposed solution incorporates a two-dimensional array of ultrasonic sensors densely distributed on a spherical surface, whereas illumination is performed coaxially through a cylindrical cavity in the array. Visualization of three-dimensional tomographic data at a frame rate of 10 images per second is enabled by parallel recording of 256 time-resolved signals for each individual laser pulse along with a highly efficient GPUbased real-time reconstruction. A liquid coupling medium (water), enclosed in a transparent membrane, is used to guarantee transmission of the optoacoustically generated waves to the ultrasonic detectors. Excitation at multiple wavelengths further allows imaging spectrally distinctive tissue chromophores such as oxygenated and deoxygenated haemoglobin. The performance is showcased by video-rate tracking of deep tissue vasculature and three-dimensional measurements of blood oxygenenation in a healthy human volunteer. The flexibility provided by the hand-held hardware design, combined with the real-time operation, makes the developed platform highly usable for both small animal research and clinical imaging in multiple indications, including cancer, inflammation, skin and cardiovascular diseases, diagnostics of lymphatic system and breast

Improved QD-BRET conjugates for detection and imaging

DOE Office of Scientific and Technical Information (OSTI.GOV)

Xing Yun; So, Min-kyung; Koh, Ai Leen

2008-08-01

Self-illuminating quantum dots, also known as QD-BRET conjugates, are a new class of quantum dot bioconjugates which do not need external light for excitation. Instead, light emission relies on the bioluminescence resonance energy transfer from the attached Renilla luciferase enzyme, which emits light upon the oxidation of its substrate. QD-BRET combines the advantages of the QDs (such as superior brightness and photostability, tunable emission, multiplexing) as well as the high sensitivity of bioluminescence imaging, thus holding the promise for improved deep tissue in vivo imaging. Although studies have demonstrated the superior sensitivity and deep tissue imaging potential, the stability ofmore » the QD-BRET conjugates in biological environment needs to be improved for long-term imaging studies such as in vivo cell tracking. In this study, we seek to improve the stability of QD-BRET probes through polymeric encapsulation with a polyacrylamide gel. Results show that encapsulation caused some activity loss, but significantly improved both the in vitro serum stability and in vivo stability when subcutaneously injected into the animal. Stable QD-BRET probes should further facilitate their applications for both in vitro testing as well as in vivo cell tracking studies.« less

Self Organizing Maps for use in Deep Inelastic Scattering

NASA Astrophysics Data System (ADS)

Askanazi, Evan

2015-04-01

Self Organizing Maps are a type of artificial neural network that has been proven to be particularly useful in solving complex problems in neural biology, engineering, robotics and physics. We are attempting to use the Self Organizing Map to solve problems and probe phenomenological patterns in subatomic physics, specifically in Deep Inelastic Scattering (DIS). In DIS there is a cross section in electron hadron scattering that is dependent on the momentum fraction x of the partons in the hadron and the momentum transfer of the virtual photon exchanged. There is a soft cross part of this cross section that currently can only be found through experimentation; this soft part is comprised of Structure Functions which in turn are comprised of the Parton Distribution Functions (PDFs). We aim to use the Self Organizing Process, or SOP, to take theoretical models of these PDFs and fit it to the previous, known data. The SOP will also be used to probe the behavior of the PDFs in particular at large x values, in order to observe how they congregate. The ability of the SOPto take multidimensional data and convert it into two dimensional output is anticipated to be particularly useful in achieving this aim.

In Situ Raman Spectroscopic Studies on Concentration of Electrolyte Salt in Lithium-Ion Batteries by Using Ultrafine Multifiber Probes.

PubMed

Yamanaka, Toshiro; Nakagawa, Hiroe; Tsubouchi, Shigetaka; Domi, Yasuhiro; Doi, Takayuki; Abe, Takeshi; Ogumi, Zempachi

2017-03-09

Lithium-ion batteries have attracted considerable attention due to their high power density. The change in concentration of salt in the electrolyte solution in lithium-ion batteries during operation causes serious degradation of battery performance. Herein, a new method of in situ Raman spectroscopy with ultrafine multifiber probes was developed to simultaneously study the concentrations of ions at several different positions in the electrolyte solution in deep narrow spaces between the electrodes in batteries. The total amount of ions in the electrolyte solution clearly changed during operation due to the low permeability of the solid-electrolyte interphase (SEI) at the anode for Li + permeation. The permeability, which is a key factor to achieve high battery performance, was improved (enhanced) by adding film-forming additives to the electrolyte solution to modify the properties of the SEI. The results provide important information for understanding and predicting phenomena occurring in a battery and for designing a superior battery. The present method is useful for analysis in deep narrow spaces in other electrochemical devices, such as capacitors. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Slow Domain Motions of an Oligomeric Protein from Deep-Sea Hyperthermophile probed by Neutron Spin Echo

NASA Astrophysics Data System (ADS)

Bhowmik, Debsindhu; Shrestha, Utsab; Dhindsa, Gurpreet; Sharp, Melissa; Stingaciu, Laura R.; Chu, Xiang-Qiang; Xiang-Qiang Chu Team

Deep-sea microorganisms have the ability to survive under extreme conditions, such as high pressure and high temperature. In this work, we used the combination of the neutron spin-echo (NSE) and the small angle neutron scattering (SANS) techniques to study the inter-domain motions of the inorganic pyrophosphate (IPPase) enzyme derived from thermostable microorganisms Thermococcus thioreducens. The IPPase has hexameric quaternary structure with molecular mass of approx. 120kDa (each subunit of 20kDa), which is a large oligomeric structure. The understanding of its slow inter-domain motions can be the key to explain how they are able to perform catalytic activity at higher temperature compared to mesophilic enzymes, thus leading to adapt to extreme environment present at the seabed. The NSE can probe these slow motions directly in the time domain up to several tens of nanoseconds at the nanometers length scales, while the corresponding structural change can be explored by the SANS. Our results provide a better picture of the local flexibility and conformational substates unique to these types of proteins, which will help us better understandthe relation between protein dynamics and their biological activities

Deep, noninvasive imaging and surgical guidance of submillimeter tumors using targeted M13-stabilized single-walled carbon nanotubes

PubMed Central

Ghosh, Debadyuti; Bagley, Alexander F.; Na, Young Jeong; Birrer, Michael J.; Bhatia, Sangeeta N.; Belcher, Angela M.

2014-01-01

Highly sensitive detection of small, deep tumors for early diagnosis and surgical interventions remains a challenge for conventional imaging modalities. Second-window near-infrared light (NIR2, 950–1,400 nm) is promising for in vivo fluorescence imaging due to deep tissue penetration and low tissue autofluorescence. With their intrinsic fluorescence in the NIR2 regime and lack of photobleaching, single-walled carbon nanotubes (SWNTs) are potentially attractive contrast agents to detect tumors. Here, targeted M13 virus-stabilized SWNTs are used to visualize deep, disseminated tumors in vivo. This targeted nanoprobe, which uses M13 to stably display both tumor-targeting peptides and an SWNT imaging probe, demonstrates excellent tumor-to-background uptake and exhibits higher signal-to-noise performance compared with visible and near-infrared (NIR1) dyes for delineating tumor nodules. Detection and excision of tumors by a gynecological surgeon improved with SWNT image guidance and led to the identification of submillimeter tumors. Collectively, these findings demonstrate the promise of targeted SWNT nanoprobes for noninvasive disease monitoring and guided surgery. PMID:25214538

Deep, noninvasive imaging and surgical guidance of submillimeter tumors using targeted M13-stabilized single-walled carbon nanotubes.

PubMed

Ghosh, Debadyuti; Bagley, Alexander F; Na, Young Jeong; Birrer, Michael J; Bhatia, Sangeeta N; Belcher, Angela M

2014-09-23

Highly sensitive detection of small, deep tumors for early diagnosis and surgical interventions remains a challenge for conventional imaging modalities. Second-window near-infrared light (NIR2, 950-1,400 nm) is promising for in vivo fluorescence imaging due to deep tissue penetration and low tissue autofluorescence. With their intrinsic fluorescence in the NIR2 regime and lack of photobleaching, single-walled carbon nanotubes (SWNTs) are potentially attractive contrast agents to detect tumors. Here, targeted M13 virus-stabilized SWNTs are used to visualize deep, disseminated tumors in vivo. This targeted nanoprobe, which uses M13 to stably display both tumor-targeting peptides and an SWNT imaging probe, demonstrates excellent tumor-to-background uptake and exhibits higher signal-to-noise performance compared with visible and near-infrared (NIR1) dyes for delineating tumor nodules. Detection and excision of tumors by a gynecological surgeon improved with SWNT image guidance and led to the identification of submillimeter tumors. Collectively, these findings demonstrate the promise of targeted SWNT nanoprobes for noninvasive disease monitoring and guided surgery.

The origins of near band-edge transitions in hexagonal boron nitride epilayers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Du, X. Z.; Li, J.; Lin, J. Y.

2016-02-01

Photoluminescence spectroscopy has been employed to probe the near band-edge transitions in hexagonal BN (h-BN) epilayers synthesized under varying ammonia flow rates. The results suggest that the quasi-donor-acceptor pair emission line at 5.3 eV is due to the transition between the nitrogen vacancy and a deep acceptor, whereas the 5.5 eV emission line is due to the recombination of an exciton bound to a deep acceptor formed by carbon impurity occupying the nitrogen site. By growing h-BN under high ammonia flow rates, nitrogen vacancy related peaks can be eliminated and epilayers exhibiting pure free exciton emission have been obtained.

Semi-inclusive deep inelastic scattering at small- x

NASA Astrophysics Data System (ADS)

Marquet, Cyrille; Xiao, Bo-Wen; Yuan, Feng

2009-11-01

We study the semi-inclusive hadron production in deep inelastic scattering at small-x. A transverse-momentum-dependent factorization is found consistent with the results calculated in the small-x approaches, such as the color-dipole framework and the color glass condensate, in the appropriate kinematic region at the lowest order. The transverse-momentum-dependent quark distribution can be studied in this process as a probe for the small-x saturation physics. Especially, the ratio of quark distributions as a function of transverse momentum at different x demonstrates strong dependence on the saturation scale. The Q2 dependence of the same ratio is also studied by applying the Collins-Soper-Sterman resummation method.

The Challenges and Opportunities for International Cooperative Radio Science; Experience with Mars Express and Venus Express Missions

NASA Technical Reports Server (NTRS)

Holmes, Dwight P.; Thompson, Tommy; Simpson, Richard; Tyler, G. Leonard; Dehant, Veronique; Rosenblatt, Pascal; Hausler, Bernd; Patzold, Martin; Goltz, Gene; Kahan, Daniel;

Fully integrated silicon probes for high-density recording of neural activity.

PubMed

Jun, James J; Steinmetz, Nicholas A; Siegle, Joshua H; Denman, Daniel J; Bauza, Marius; Barbarits, Brian; Lee, Albert K; Anastassiou, Costas A; Andrei, Alexandru; Aydın, Çağatay; Barbic, Mladen; Blanche, Timothy J; Bonin, Vincent; Couto, João; Dutta, Barundeb; Gratiy, Sergey L; Gutnisky, Diego A; Häusser, Michael; Karsh, Bill; Ledochowitsch, Peter; Lopez, Carolina Mora; Mitelut, Catalin; Musa, Silke; Okun, Michael; Pachitariu, Marius; Putzeys, Jan; Rich, P Dylan; Rossant, Cyrille; Sun, Wei-Lung; Svoboda, Karel; Carandini, Matteo; Harris, Kenneth D; Koch, Christof; O'Keefe, John; Harris, Timothy D

2017-11-08

Sensory, motor and cognitive operations involve the coordinated action of large neuronal populations across multiple brain regions in both superficial and deep structures. Existing extracellular probes record neural activity with excellent spatial and temporal (sub-millisecond) resolution, but from only a few dozen neurons per shank. Optical Ca 2+ imaging offers more coverage but lacks the temporal resolution needed to distinguish individual spikes reliably and does not measure local field potentials. Until now, no technology compatible with use in unrestrained animals has combined high spatiotemporal resolution with large volume coverage. Here we design, fabricate and test a new silicon probe known as Neuropixels to meet this need. Each probe has 384 recording channels that can programmably address 960 complementary metal-oxide-semiconductor (CMOS) processing-compatible low-impedance TiN sites that tile a single 10-mm long, 70 × 20-μm cross-section shank. The 6 × 9-mm probe base is fabricated with the shank on a single chip. Voltage signals are filtered, amplified, multiplexed and digitized on the base, allowing the direct transmission of noise-free digital data from the probe. The combination of dense recording sites and high channel count yielded well-isolated spiking activity from hundreds of neurons per probe implanted in mice and rats. Using two probes, more than 700 well-isolated single neurons were recorded simultaneously from five brain structures in an awake mouse. The fully integrated functionality and small size of Neuropixels probes allowed large populations of neurons from several brain structures to be recorded in freely moving animals. This combination of high-performance electrode technology and scalable chip fabrication methods opens a path towards recording of brain-wide neural activity during behaviour.

Fully Integrated Silicon Probes for High-Density Recording of Neural Activity

PubMed Central

Jun, James J.; Steinmetz, Nicholas A.; Siegle, Joshua H.; Denman, Daniel J.; Bauza, Marius; Barbarits, Brian; Lee, Albert K.; Anastassiou, Costas A.; Andrei, Alexandru; Aydın, Çağatay; Barbic, Mladen; Blanche, Timothy J.; Bonin, Vincent; Couto, João; Dutta, Barundeb; Gratiy, Sergey L.; Gutnisky, Diego A.; Häusser, Michael; Karsh, Bill; Ledochowitsch, Peter; Lopez, Carolina Mora; Mitelut, Catalin; Musa, Silke; Okun, Michael; Pachitariu, Marius; Putzeys, Jan; Rich, P. Dylan; Rossant, Cyrille; Sun, Wei-lung; Svoboda, Karel; Carandini, Matteo; Harris, Kenneth D.; Koch, Christof; O'Keefe, John; Harris, Timothy D.

2018-01-01

Summary Paragraph Sensory, motor, and cognitive operations involve the coordinated action of large neuronal populations across multiple brain regions in both superficial and deep structures1,2. Existing extracellular probes record neural activity with excellent spatial and temporal (sub-millisecond) resolution but from only a few dozen neurons per shank. Optical Ca2+ imaging3–5 offers more coverage but lacks the temporal resolution to reliably distinguish individual spikes and does not measure local field potentials. To date, no technology compatible with unrestrained animals has combined high spatiotemporal resolution with large volume coverage. To satisfy this need, we designed, fabricated, and tested a new silicon probe called Neuropixels. Each probe has 384 recording channels that can programmably address 960 CMOS processing-compatible low-impedance TiN6 sites that tile a single 10 mm long, 70x20 µm cross section shank. The 6x9 mm probe base is fabricated with the shank on a single chip. Voltage signals are filtered, amplified, multiplexed, and digitized on the base, allowing noise-free digital data transmission directly from the probe. The combination of dense recording sites and high channel count yielded well-isolated spiking activity from hundreds of neurons per probe implanted in mice and rats. Using two probes, more than 700 well-isolated single neurons were simultaneously recorded from five brain structures in an awake mouse. The fully integrated functionality and small size of Neuropixels probes allowed recording large populations of neurons from multiple brain structures in freely moving animals. This combination of high-performance electrode technology and scalable chip fabrication methods opens the path to record brain-wide neural activity during behavior. PMID:29120427

A Coaxial Dual-element Focused Ultrasound Probe for Guidance of Epidural Catheterization: An Experimental Study.

PubMed

Dong, Guo-Chung; Chiu, Li-Chen; Ting, Chien-Kun; Hsu, Jia-Ruei; Huang, Chih-Chung; Chang, Yin; Chen, Gin-Shin

2017-09-01

Ultrasound guidance for epidural block has improved clinical blind-trial problems but the design of present ultrasonic probes poses operating difficulty of ultrasound-guided catheterization, increasing the failure rate. The purpose of this study was to develop a novel ultrasonic probe to avoid needle contact with vertebral bone during epidural catheterization. The probe has a central circular passage for needle insertion. Two focused annular transducers are deployed around the passage for on-axis guidance. A 17-gauge insulated Tuohy needle containing the self-developed fiber-optic-modified stylet was inserted into the back of the anesthetized pig, in the lumbar region under the guidance of our ultrasonic probe. The inner transducer of the probe detected the shallow echo signals of the peak-peak amplitude of 2.8 V over L3 at the depth of 2.4 cm, and the amplitude was decreased to 0.8 V directly over the L3 to L4 interspace. The outer transducer could detect the echoes from the deeper bone at the depth of 4.5 cm, which did not appear for the inner transducer. The operator tilted the probe slightly in left-right and cranial-caudal directions until the echoes at the depth of 4.5 cm disappeared, and the epidural needle was inserted through the central passage of the probe. The needle was advanced and stopped when the epidural space was identified by optical technique. The needle passed without bone contact. Designs of the hollow probe for needle pass and dual transducers with different focal lengths for detection of shallow and deep vertebrae may benefit operation, bone/nonbone identification, and cost.

PET-Probe: Evaluation of Technical Performance and Clinical Utility of a Handheld High-Energy Gamma Probe in Oncologic Surgery.

PubMed

Gulec, Seza A; Daghighian, Farhad; Essner, Richard

2016-12-01

Positron emission tomography (PET) has become an invaluable part of patient evaluation in surgical oncology. PET is less than optimal for detecting lesions <1 cm, and the intraoperative localization of small PET-positive lesions can be challenging as a result of difficulties in surgical exposure. We undertook this investigation to assess the utility of a handheld high-energy gamma probe (PET-Probe) for intraoperative identification of 18 F-deoxyglucose (FDG)-avid tumors. Forty patients underwent a diagnostic whole-body FDG-PET scan for consideration for surgical exploration and resection. Before surgery, all patients received an intravenous injection of 7 to 10 mCi of FDG. At surgery, the PET-Probe was used to determine absolute counts per second at the known tumor site(s) demonstrated by whole-body PET and at adjacent normal tissue (at least 4 cm away from tumor-bearing sites). Tumor-to-background ratios were calculated. Thirty-two patients (80%) underwent PET-Probe-guided surgery with therapeutic intent in a recurrent or metastatic disease setting. Eight patients underwent surgery for diagnostic exploration. Anatomical locations of the PET-identified lesions were neck and supraclavicular (n = 8), axilla (n = 5), groin and deep iliac (n = 4), trunk and extremity soft tissue (n = 3), abdominal and retroperitoneal (n = 19), and lung (n = 2). PET-Probe detected all PET-positive lesions. The PET-Probe was instrumental in localization of lesions in 15 patients that were not immediately apparent by surgical exploration. The PET-Probe identified all lesions demonstrated by PET scanning and, in selected cases, was useful in localizing FDG-avid disease not seen with conventional PET scanning.

Development of a novel gamma probe for detecting radiation direction

NASA Astrophysics Data System (ADS)

Pani, R.; Pellegrini, R.; Cinti, M. N.; Longo, M.; Donnarumma, R.; D'Alessio, A.; Borrazzo, C.; Pergola, A.; Ridolfi, S.; De Vincentis, G.

2016-01-01

Spatial localization of radioactive sources is currently a main issue interesting different fields, including nuclear industry, homeland security as well as medical imaging. It is currently achieved using different systems, but the development of technologies for detecting and characterizing radiation is becoming important especially in medical imaging. In this latter field, radiation detection probes have long been used to guide surgery, thanks to their ability to localize and quantify radiopharmaceutical uptake even deep in tissue. Radiolabelled colloid is injected into, or near to, the tumor and the surgeon uses a hand-held radiation detector, the gamma probe, to identify lymph nodes with radiopharmaceutical uptkake. The present work refers to a novel scintigraphic goniometric probe to identify gamma radiation and its direction. The probe incorporates several scintillation crystals joined together in a particular configuration to provide data related to the position of a gamma source. The main technical characteristics of the gamma locator prototype, i.e. sensitivity, spatial resolution and detection efficiency, are investigated. Moreover, the development of a specific procedure applied to the images permits to retrieve the source position with high precision with respect to the currently used gamma probes. The presented device shows a high sensitivity and efficiency to identify gamma radiation taking a short time (from 30 to 60 s). Even though it was designed for applications in radio-guided surgery, it could be used for other purposes, as for example homeland security.

A multi-channel capacitive probe for electrostatic fluctuation measurement in the Madison Symmetric Torus reversed field pinch.

PubMed

Tan, Mingsheng; Stone, Douglas R; Triana, Joseph C; Almagri, Abdulgader F; Fiksel, Gennady; Ding, Weixing; Sarff, John S; McCollam, Karsten J; Li, Hong; Liu, Wandong

2017-02-01

A 40-channel capacitive probe has been developed to measure the electrostatic fluctuations associated with the tearing modes deep into Madison Symmetric Torus (MST) reversed field pinch plasma. The capacitive probe measures the ac component of the plasma potential via the voltage induced on stainless steel electrodes capacitively coupled with the plasma through a thin annular layer of boron nitride (BN) dielectric (also serves as the particle shield). When bombarded by the plasma electrons, BN provides a sufficiently large secondary electron emission for the induced voltage to be very close to the plasma potential. The probe consists of four stalks each with ten cylindrical capacitors that are radially separated by 1.5 cm. The four stalks are arranged on a 1.3 cm square grid so that at each radial position, there are four electrodes forming a square grid. Every two adjacent radial sets of four electrodes form a cube. The fluctuating electric field can be calculated by the gradient of the plasma potential fluctuations at the eight corners of the cube. The probe can be inserted up to 15 cm (r/a = 0.7) into the plasma. The capacitive probe has a frequency bandwidth from 13 Hz to 100 kHz, amplifier-circuit limit, sufficient for studying the tearing modes (5-30 kHz) in the MST reversed-field pinch.

Differential impact of thalamic versus subthalamic deep brain stimulation on lexical processing.

PubMed

Krugel, Lea K; Ehlen, Felicitas; Tiedt, Hannes O; Kühn, Andrea A; Klostermann, Fabian

2014-10-01

Roles of subcortical structures in language processing are vague, but, interestingly, basal ganglia and thalamic Deep Brain Stimulation can go along with reduced lexical capacities. To deepen the understanding of this impact, we assessed word processing as a function of thalamic versus subthalamic Deep Brain Stimulation. Ten essential tremor patients treated with thalamic and 14 Parkinson׳s disease patients with subthalamic Deep Brain Stimulation performed an acoustic Lexical Decision Task ON and OFF stimulation. Combined analysis of task performance and event-related potentials allowed the determination of processing speed, priming effects, and N400 as neurophysiological correlate of lexical stimulus processing. 12 age-matched healthy participants acted as control subjects. Thalamic Deep Brain Stimulation prolonged word decisions and reduced N400 potentials. No comparable ON-OFF effects were present in patients with subthalamic Deep Brain Stimulation. In the latter group of patients with Parkinson' disease, N400 amplitudes were, however, abnormally low, whether under active or inactive Deep Brain Stimulation. In conclusion, performance speed and N400 appear to be influenced by state functions, modulated by thalamic, but not subthalamic Deep Brain Stimulation, compatible with concepts of thalamo-cortical engagement in word processing. Clinically, these findings specify cognitive sequels of Deep Brain Stimulation in a target-specific way. Copyright © 2014 Elsevier Ltd. All rights reserved.

The MUSE Hubble Ultra Deep Field Survey. IX. Evolution of galaxy merger fraction since z ≈ 6

NASA Astrophysics Data System (ADS)

Ventou, E.; Contini, T.; Bouché, N.; Epinat, B.; Brinchmann, J.; Bacon, R.; Inami, H.; Lam, D.; Drake, A.; Garel, T.; Michel-Dansac, L.; Pello, R.; Steinmetz, M.; Weilbacher, P. M.; Wisotzki, L.; Carollo, M.

2017-11-01

We provide, for the first time, robust observational constraints on the galaxy major merger fraction up to z ≈ 6 using spectroscopic close pair counts. Deep Multi Unit Spectroscopic Explorer (MUSE) observations in the Hubble Ultra Deep Field (HUDF) and Hubble Deep Field South (HDF-S) are used to identify 113 secure close pairs of galaxies among a parent sample of 1801 galaxies spread over a large redshift range (0.2 < z < 6) and stellar masses (107-1011 M⊙), thus probing about 12 Gyr of galaxy evolution. Stellar masses are estimated from spectral energy distribution (SED) fitting over the extensive UV-to-NIR HST photometry available in these deep Hubble fields, adding Spitzer IRAC bands to better constrain masses for high-redshift (z ⩾ 3) galaxies. These stellar masses are used to isolate a sample of 54 major close pairs with a galaxy mass ratio limit of 1:6. Among this sample, 23 pairs are identified at high redshift (z ⩾ 3) through their Lyα emission. The sample of major close pairs is divided into five redshift intervals in order to probe the evolution of the merger fraction with cosmic time. Our estimates are in very good agreement with previous close pair counts with a constant increase of the merger fraction up to z ≈ 3 where it reaches a maximum of 20%. At higher redshift, we show that the fraction slowly decreases down to about 10% at z ≈ 6. The sample is further divided into two ranges of stellar masses using either a constant separation limit of 109.5 M⊙ or the median value of stellar mass computed in each redshift bin. Overall, the major close pair fraction for low-mass and massive galaxies follows the same trend. These new, homogeneous, and robust estimates of the major merger fraction since z ≈ 6 are in good agreement with recent predictions of cosmological numerical simulations. Based on observations made with ESO telescopes at the La Silla-Paranal Observatory under programmes 094.A-0289(B), 095.A-0010(A), 096.A-0045(A) and 096.A-0045(B).

Physical effects involved in the measurements of neutrino masses with future cosmological data

DOE Office of Scientific and Technical Information (OSTI.GOV)

Archidiacono, Maria; Brinckmann, Thejs; Lesgourgues, Julien

Future Cosmic Microwave Background experiments together with upcoming galaxy and 21-cm surveys will provide extremely accurate measurements of different cosmological observables located at different epochs of the cosmic history. The new data will be able to constrain the neutrino mass sum with the best precision ever. In order to exploit the complementarity of the different redshift probes, a deep understanding of the physical effects driving the impact of massive neutrinos on CMB and large scale structures is required. The goal of this work is to describe these effects, assuming a summed neutrino mass close to its minimum allowed value. Wemore » find that parameter degeneracies can be removed by appropriate combinations, leading to robust and model independent constraints. A joint forecast of the sensitivity of Euclid and DESI surveys together with a CORE-like CMB experiment leads to a 1σ uncertainty of 14 meV on the summed neutrino mass. Finally the degeneracy between M {sub ν} and the optical depth at reionization τ{sub reio}, originating in the combination of CMB and low redshift galaxy probes, might be broken by future 21-cm surveys, thus further decreasing the uncertainty on M {sub ν}. For instance, an independent determination of the optical depth with an accuracy of σ(τ{sub reio})=0.001 (which might be achievable, although this is subject to astrophysical uncertainties) would decrease the uncertainty down to σ( M {sub ν})=12 meV.« less

Will Deep Impact Make a Splash?

NASA Technical Reports Server (NTRS)

Sheldon, Robert B.; Hoover, Richard B.

2005-01-01

Recent cometary observations from spacecraft flybys support the hypothesis that short-period comets have been substantially modified by the presence of liquid water. Such a model can resolve many outstanding questions of cometary dynamics, as well as the differences between the flyby observations and the dirty snowball paradigm. The model also predicts that the Deep Impact mission, slated for a July 4, 2005 collision with Comet Temple-1, will encounter a layered, heterogenous nucleus with subsurface liquid water capped by dense crust. Collision ejecta will include not only vaporized material, but liquid water and large pieces of crust. Since the water will immediately boil, we predict that the water vapor signature of Deep Impact may be an order of magnitude larger than that expected from collisional vaporization alone.

Deep Impact comet encounter: design, development, and operations of the Big Event at Tempel 1

NASA Technical Reports Server (NTRS)

Wissler, Steven

2005-01-01

Deep Impact is NASA's eighth Discovery mission. This low-cost, focused planetary science investigation gathered the data necessary to help scientists unlock early secrets of our solar system. The comet encounter with Tempel 1 was a complex event - requiring extremely accurate timing, robutstness to an unknown environment, and flight team adaptability. The mission operations and flight systems performance were spectacular for approach, impact, and lookback imaging on July 4, 2005.

Deep Impact comet encounter: design, development, and operations of the big event at Tempel 1

NASA Technical Reports Server (NTRS)

Wissler, Steven; Rocca, Jennifer; Kubitschek, Daniel

2005-01-01

Deep Impact is NASA's eighth Discovery mission. This low-cost, focused planetary science investigation gathered the data necessary to help scientists unlock early secrets of our solar system. The comet encounter with Tempel 1 was a complex event - requiring extremely accurate timing, robustness to an unknown environment, and flight team adaptibility. The mission operations and flight systems performance were spectacular for approach, impact, and lookback imaging on July 4, 2005.

Super-deep diamond genesis at Redox conditions of slab-mantle boundary

NASA Astrophysics Data System (ADS)

Gao, J.; Chen, B.; Wu, X.

2017-12-01

Diamond genesis is an intriguing issue for diamond resources and the deep carbon cycle of the Earth's interiors. Super-deep diamonds, representing only 6% of the global diamond population, often host inclusions with phase assemblages requiring a sublithospheric origin (>300 km). Being the windows for probing the deep Earth, super-deep diamonds with their distinctive micro-inclusions not only record a history of oceanic lithosphere subduction and upward transport at a depth of >250 km to even 1000 km, but indicate their genesis pertinent to mantle-carbonate melts in a Fe0-bufferred reduced condition. Our pilot experiments have evidenced the formation of diamonds from MgCO3-Fe0 system in a diamond anvil cell device at 25 GPa and 1800 K. Detailed experimental investigations of redox mechanism of MgCO3-Fe0 and CaCO3-Fe0 coupling have been conducted using multi-anvil apparatus. The conditions are set along the oceanic lithosphere subduction paths in the pressure-temperature range of 10-24 GPa and 1200-2000 K, covering the formation region of most super-deep diamonds. The clear reaction zones strongly support the redox reaction between carbonatitic slab and Fe0-bearing metals under mantle conditions. Our study has experimentally documented the possibility of super-deep diamond genesis at redox conditions of carbonateitic slab and Fe0-bearings. The kinetics of diamond formation as a function of pressure-temperature conditions are also discussed.

Drivers in the Scaling Between Precipitation and Cloud Radiative Impacts in Deep Convection

NASA Astrophysics Data System (ADS)

Rapp, A. D.; Sun, L.; Smalley, K.

2017-12-01

The coupling between changes in radiation and precipitation has been demonstrated by a number of studies and suggests an important link between cloud and precipitation processes for defining climate sensitivity. Precipitation and radiative fluxes from CloudSat/CALIPSO retrieval products are used to examine the relationship between precipitation and cloud radiative impacts through two dimensionless parameters. The surface radiative cooling impact, Rc, represents the ratio of the surface shortwave cloud radiative effect to latent heating (LH) from precipitation. The atmospheric radiative heating impact, Rh, represents the ratio of the atmospheric cloud radiative effect to LH from precipitation. Together, these parameters describe the relationship between precipitation processes and how efficiently clouds cools the surface or heats the atmosphere. Deep convective clouds are identified using the 2B-GEOPROF-LIDAR joint radar-lidar product and the cloud radiative impact parameters are calculated from the 2B-FLXHR-LIDAR fluxes and 2C-RAIN-PROFILE precipitation. Deep convective clouds will be sampled according to their dynamic and thermodynamic regimes to provide insights into the factors that control the scaling between precipitation and radiative impacts. Preliminary results from analysis of precipitating deep convective pixels indicates a strong increase (decrease) in the ratio of atmospheric heating (surface cooling) and precipitation with thermodynamic environment, especially increasing water vapor; however, it remains to be seen whether these results hold when integrated over an entire deep convective cloud system. Analysis of the dependence of Rc and Rh on the cloud horizontal and vertical structure is also planned, which should lead to a better understanding of the role of non-precipitating anvil characteristics in modulating the relationship between precipitation and surface and atmospheric radiative effects.

Plastic microfibre ingestion by deep-sea organisms

PubMed Central

Taylor, M. L.; Gwinnett, C.; Robinson, L. F.; Woodall, L. C.

2016-01-01

Plastic waste is a distinctive indicator of the world-wide impact of anthropogenic activities. Both macro- and micro-plastics are found in the ocean, but as yet little is known about their ultimate fate and their impact on marine ecosystems. In this study we present the first evidence that microplastics are already becoming integrated into deep-water organisms. By examining organisms that live on the deep-sea floor we show that plastic microfibres are ingested and internalised by members of at least three major phyla with different feeding mechanisms. These results demonstrate that, despite its remote location, the deep sea and its fragile habitats are already being exposed to human waste to the extent that diverse organisms are ingesting microplastics. PMID:27687574

Work on Planetary Atmospheres and Planetary Atmosphere Probes

NASA Technical Reports Server (NTRS)

Lester, Peter

1999-01-01

A summary final report of work accomplished is presented. Work was performed in the following areas: (1) Galileo Probe science analysis, (2) Galileo probe Atmosphere Structure Instrument, (3) Mars Pathfinder Atmosphere Structure/Meteorology instrument, (4) Mars Pathfinder data analysis, (5) Science Definition for future Mars missions, (6) Viking Lander data analysis, (7) winds in Mars atmosphere Venus atmospheric dynamics, (8) Pioneer Venus Probe data analysis, (9) Pioneer Venus anomaly analysis, (10) Discovery Venus Probe Titan probe instrument design, and (11) laboratory studies of Titan probe impact phenomena. The work has resulted in more than 10 articles published in archive journals, 2 encyclopedia articles, and many working papers. This final report is organized around the four planets on which there was activity, Jupiter, Mars, Venus, and Titan, with a closing section on Miscellaneous Activities. A major objective was to complete the fabrication, test, and evaluation of the atmosphere structure experiment on the Galileo probe, and to receive, analyze and interpret data received from the spacecraft. The instrument was launched on April 14, 1989. Calibration data were taken for all experiment sensors. The data were analyzed, fitted with algorithms, and summarized in a calibration report for use in analyzing and interpreting data returned from Jupiter's atmosphere. The sensors included were the primary science pressure, temperature and acceleration sensors, and the supporting engineering temperature sensors. Computer programs were written to decode the Experiment Data Record and convert the digital numbers to physical quantities, i.e., temperatures, pressures, and accelerations. The project office agreed to obtain telemetry of checkout data from the probe. Work to extend programs written for use on the Pioneer Venus project included: (1) massive heat shield ablation leading to important mass loss during entry; and (2) rapid planet rotation, which introduced terms of motion not needed on Venus. When the Galileo Probe encountered Jupiter, analysis and interpretation of data commenced. The early contributions of the experiment were to define (1) the basic structure of the deep atmosphere, (2) the stability of the atmosphere, (3) the upper atmospheric profiles of density, pressure, and temperature. The next major task in the Galileo Probe project was to refine, verify and extend the analysis of the data. It was the verified, and corrected data, which indicated a dry abiabatic atmosphere within measurement accuracy. Temperature in the thermosphere was measured at 900 K. Participation in the Mars atmospheric research included: (1) work as a team member of the Mars Atmosphere Working Group, (2) contribution to the Mars Exobiology Instrument workshop, (3) asssistance in planning the Mars global network and (4) assitance in planning the Soviet-French Mars mission in 1994. This included a return to the Viking Lander parachute data to refine and improve the definition of winds between 1.5 and 4 kilometer altitude at the two entry sites. The variability of the structure of Mars atmosphere was addressed, which is known to vary with season, latitude, hemisphere and dust loading of the atmosphere. This led to work on the Pathfinder project. The probe had a deployable meteorology mast that had three temperature sensors, and a wind sensor at the tip of the mast. Work on the Titan atmospheric probe was also accomplished. This included developing an experiment proposal to the European Space Agency (ESA), which was not selected. However, as an advisor in the design and preparation of the selected experiment the researcher interacted with scientist on the Huygens Probe Atmosphere Structure Experiment. The researcher also participated in the planning for the Venus Chemical Probe. The science objectives of the probe were to resolve unanswered questions concerning the minor species chemistry of Venus' atmosphere that control cloud formation, greenhouse effectiveness, and the thermal structure. The researcher also reviewed problems with the Pioneer Venus Probe, that caused anomalies which occurred on the Probes at and below 12.5 km level of the Venus' atmosphere. He convened and participated in a workshop that concluded the most likely hardware cause was insulation failure in the electrical harness outside the Probes' pressure vessels. It was discovered that the shrink tubing material failed at 600K. This failure could explain the anomalies experienced by the probes. The descent data of the Pioneer probes, and the Soviet Vega Lander was analyzed to evaluate the presence of small scale gravity waves in and below the Venus cloud layer.

Development of a Compact, Deep-Penetrating Heat Flow Instrument for Lunar Landers: In-Situ Thermal Conductivity System

NASA Technical Reports Server (NTRS)

Nagihara, S.; Zacny, K.; Hedlund, M.; Taylor, P. T.

2012-01-01

Geothermal heat flow is obtained as a product of the geothermal gradient and the thermal conductivity of the vertical soil/rock/regolith interval penetrated by the instrument. Heat flow measurements are a high priority for the geophysical network missions to the Moon recommended by the latest Decadal Survey and previously the International Lunar Network. One of the difficulties associated with lunar heat flow measurement on a robotic mission is that it requires excavation of a relatively deep (approx 3 m) hole in order to avoid the long-term temporal changes in lunar surface thermal environment affecting the subsurface temperature measurements. Such changes may be due to the 18.6-year-cylcle lunar precession, or may be initiated by presence of the lander itself. Therefore, a key science requirement for heat flow instruments for future lunar missions is to penetrate 3 m into the regolith and to measure both thermal gradient and thermal conductivity. Engineering requirements are that the instrument itself has minimal impact on the subsurface thermal regime and that it must be a low-mass and low-power system like any other science instrumentation on planetary landers. It would be very difficult to meet the engineering requirements, if the instrument utilizes a long (> 3 m) probe driven into the ground by a rotary or percussive drill. Here we report progress in our efforts to develop a new, compact lunar heat flow instrumentation that meets all of these science and engineering requirements.

49 CFR 572.16 - Head.

Code of Federal Regulations, 2012 CFR

2012-10-01

... SA 103C 002, sheet 8. (b) When the head is impacted by a test probe specified in § 572.21(a)(1) at 7... supports the limbs' weight when the limbs are extended horizontally forward. (2) Adjust the test probe so... the forehead immediately adjacent to the projected longitudinal centerline of the test probe is...

49 CFR 572.8 - Thorax.

Code of Federal Regulations, 2012 CFR

2012-10-01

... in paragraph (d)(7) of this section. (c) When impacted by a test probe conforming to § 572.11(a) at... forces measured by the test probe of not more than 1450 pounds and 2250 pounds, respectively, and shall... forward parallel to the midsagittal plane. (2) Place the longitudinal center line of the test probe so...

49 CFR 572.16 - Head.

Code of Federal Regulations, 2010 CFR

2010-10-01

... SA 103C 002, sheet 8. (b) When the head is impacted by a test probe specified in § 572.21(a)(1) at 7... supports the limbs' weight when the limbs are extended horizontally forward. (2) Adjust the test probe so... the forehead immediately adjacent to the projected longitudinal centerline of the test probe is...

49 CFR 572.8 - Thorax.

Code of Federal Regulations, 2011 CFR

2011-10-01

... in paragraph (d)(7) of this section. (c) When impacted by a test probe conforming to § 572.11(a) at... forces measured by the test probe of not more than 1450 pounds and 2250 pounds, respectively, and shall... forward parallel to the midsagittal plane. (2) Place the longitudinal center line of the test probe so...

49 CFR 572.8 - Thorax.

Code of Federal Regulations, 2013 CFR

2013-10-01

... in paragraph (d)(7) of this section. (c) When impacted by a test probe conforming to § 572.11(a) at... forces measured by the test probe of not more than 1450 pounds and 2250 pounds, respectively, and shall... forward parallel to the midsagittal plane. (2) Place the longitudinal center line of the test probe so...

49 CFR 572.8 - Thorax.

Code of Federal Regulations, 2014 CFR

2014-10-01

... in paragraph (d)(7) of this section. (c) When impacted by a test probe conforming to § 572.11(a) at... forces measured by the test probe of not more than 1450 pounds and 2250 pounds, respectively, and shall... forward parallel to the midsagittal plane. (2) Place the longitudinal center line of the test probe so...

49 CFR 572.16 - Head.

Code of Federal Regulations, 2013 CFR

2013-10-01

... SA 103C 002, sheet 8. (b) When the head is impacted by a test probe specified in § 572.21(a)(1) at 7... supports the limbs' weight when the limbs are extended horizontally forward. (2) Adjust the test probe so... the forehead immediately adjacent to the projected longitudinal centerline of the test probe is...

49 CFR 572.16 - Head.

Code of Federal Regulations, 2011 CFR

2011-10-01

... SA 103C 002, sheet 8. (b) When the head is impacted by a test probe specified in § 572.21(a)(1) at 7... supports the limbs' weight when the limbs are extended horizontally forward. (2) Adjust the test probe so... the forehead immediately adjacent to the projected longitudinal centerline of the test probe is...

49 CFR 572.8 - Thorax.

Code of Federal Regulations, 2010 CFR

2010-10-01

... in paragraph (d)(7) of this section. (c) When impacted by a test probe conforming to § 572.11(a) at... forces measured by the test probe of not more than 1450 pounds and 2250 pounds, respectively, and shall... forward parallel to the midsagittal plane. (2) Place the longitudinal center line of the test probe so...

49 CFR 572.16 - Head.

Code of Federal Regulations, 2014 CFR

2014-10-01

... SA 103C 002, sheet 8. (b) When the head is impacted by a test probe specified in § 572.21(a)(1) at 7... supports the limbs' weight when the limbs are extended horizontally forward. (2) Adjust the test probe so... the forehead immediately adjacent to the projected longitudinal centerline of the test probe is...

Transcardiac conducted electrical weapon (TASER) probe deployments: incidence and outcomes.

PubMed

Bozeman, William P; Teacher, Eric; Winslow, James E

2012-12-01

TASER (TASER International, Scottsdale, AZ) conducted electrical weapons (CEWs) are commonly used by law enforcement officers. Although animal studies have suggested that transcardiac CEW discharges may produce direct cardiac effects, this has not been demonstrated in human studies. This study sought to determine the incidence and outcomes of transcardiac CEW probe impact locations in a large series of actual CEW deployments. A multi-center database of consecutive CEW uses by law enforcement officers was retrospectively reviewed. Case report forms were independently reviewed by three investigators to identify cases with paired probe configurations potentially producing a transcardiac discharge vector. Descriptive analysis was performed and inter-rater reliability was assessed. Among 1201 total CEW uses, 813 included probe deployments and 178 cases had paired anterior probe impacts potentially capable of producing a transcardiac discharge vector. This represents 14.8% of all CEW uses (95% confidence interval [CI] 12.9-16.9%) and 21.9% of CEW uses in probe mode (95% CI 19.1-24.9%). Inter-rater agreement was very good, with kappa = 0.82. There were no immediate deaths in any cases (97.5% CI 0.0-0.3%) to suggest a cardiac dysrhythmia, including those with transcardiac discharge vector. CEW deployments with probe impact configurations capable of producing a transcardiac discharge occur in a minority of cases in field use conditions. None of these cases, transcardiac or otherwise, produced immediately fatal dysrhythmias. These data support the overall safety of CEWs and provide a benchmark estimate of the likelihood of transcardiac discharge vectors occurring in field use of CEWs. Copyright © 2012 Elsevier Inc. All rights reserved.

Hollow Cathode and Keeper-region Plasma Measurements Using Ultra-fast Miniature Scanning Probes

NASA Technical Reports Server (NTRS)

Goebel, Dan M.; Jameson, Kristina K.; Watkins, Ron M.; Katz, Ira

2004-01-01

In order to support the development of comprehensive performance and life models for future deep space missions that will utilize ion thrusters, we have undertaken a study of the plasma structure in hollow cathodes using an new pneumatic scanning probe diagnostic. This device is designed to insert a miniature probe directly into the hollow cathode orifice from either the upstream insert region in the interior of the hollow cathode, or from the downstream keeper-plasma region at the exit of the hollow cathode, to provide complete axial profiles of the discharge plasma parameters. Previous attempts to diagnose this region with probes was Limited by the melting of small probes in the intense discharge near the orifice, or caused significant perturbation of the plasma by probes large enough to survive. Our new probe is extremely compact, and when configured as a single Langmuir probe, the ceramic tube insulator is only 0.5mm in diameter and the current collecting conductor has a total area of 0.002 cm2. A series of current-voltage characteristics are obtained by applying a rapid sawtooth voltage waveform to the probe as it is scanned by the pneumatic actuator into and out of the plasma region, The bellow-sealed pneumatic drive scans the probe 4 cm in the cathode insert region and 10 cm in the anode/keeper plasmas region at average speeds of about 1 mm/msec, and the residence time at the end of the insertion stroke in the densest part of the plasma near the orifice is measured to be only 10 msec. Since the voltage sweep time is fast compared to the motion of the probe, axial profiles of the plasma density, temperature and potential with reasonable spatial resolution are obtained. Measurements of the internal cathode pressures and the axial plasma-parameter profiles for a hollow cathode operating at discharge currents of up to 35 A in xenon will be presented.

Unusual Fluorescent Responses of Morpholine-functionalized Fluorescent Probes to pH via Manipulation of BODIPY’s HOMO and LUMO Energy Orbitals for Intracellular pH Detection

PubMed Central

Zhang, Jingtuo; Yang, Mu; Mazi, Wafa; Adhikari, Kapil; Fang, Mingxi; Xie, Fei; Valenzano, Loredana; Tiwari, Ashutosh; Luo, Fen-Tair; Liu, Haiying

2016-01-01

Three uncommon morpholine-based fluorescent probes (A, B and C) for pH were prepared by introducing morpholine residues to BODIPY dyes at 4,4’- and 2,6-positions, respectively. In contrast to morpholine-based fluorescent probes for pH reported in literature, these fluorescent probes display high fluorescence in a basic condition while they exhibit very weak fluorescence in an acidic condition. The theoretical calculation confirmed that morpholine is unable to function as either an electron donor or an electron acceptor to quench the BODIPY fluorescence in the neutral and basic condition via photo-induced electron transfer (PET) mechanism because the LUMO energy of morpholine is higher than those of the BODIPY dyes while its HOMO energy is lower than those of the BODIPY dyes. However, the protonation of tertiary amines of the morpholine residues in an acidic environment leads to fluorescence quenching of the BODIPY dyes via d-PET mechanism. The fluorescence quenching is because the protonation effectively decreases the LUMO energy which locates between the HOMO and LUMO energies of the BODIPY dyes. Fluorescent probe C with deep-red emission has been successfully used to detect pH changes in mammalian cells. PMID:27547822

Layer-by-layer assembled fluorescent probes in the second near-infrared window for systemic delivery and detection of ovarian cancer

PubMed Central

Dang, Xiangnan; Gu, Li; Qi, Jifa; Correa, Santiago; Zhang, Geran; Belcher, Angela M.; Hammond, Paula T.

2016-01-01

Fluorescence imaging in the second near-infrared window (NIR-II, 1,000–1,700 nm) features deep tissue penetration, reduced tissue scattering, and diminishing tissue autofluorescence. Here, NIR-II fluorescent probes, including down-conversion nanoparticles, quantum dots, single-walled carbon nanotubes, and organic dyes, are constructed into biocompatible nanoparticles using the layer-by-layer (LbL) platform due to its modular and versatile nature. The LbL platform has previously been demonstrated to enable incorporation of diagnostic agents, drugs, and nucleic acids such as siRNA while providing enhanced blood plasma half-life and tumor targeting. This work carries out head-to-head comparisons of currently available NIR-II probes with identical LbL coatings with regard to their biodistribution, pharmacokinetics, and toxicities. Overall, rare-earth-based down-conversion nanoparticles demonstrate optimal biological and optical performance and are evaluated as a diagnostic probe for high-grade serous ovarian cancer, typically diagnosed at late stage. Successful detection of orthotopic ovarian tumors is achieved by in vivo NIR-II imaging and confirmed by ex vivo microscopic imaging. Collectively, these results indicate that LbL-based NIR-II probes can serve as a promising theranostic platform to effectively and noninvasively monitor the progression and treatment of serous ovarian cancer. PMID:27114520

Magnetic resonance imaging-guided focused laser interstitial thermal therapy for subinsular metastatic adenocarcinoma: technical case report.

PubMed

Hawasli, Ammar H; Ray, Wilson Z; Murphy, Rory K J; Dacey, Ralph G; Leuthardt, Eric C

2012-06-01

To describe the novel use of the AutoLITT System (Monteris Medical, Winnipeg, Manitoba, Canada) for focused laser interstitial thermal therapy (LITT) with intraoperative magnetic resonance imaging (MRI) and stereotactic image guidance for the treatment of metastatic adenocarcinoma in the left insula. The patient was a 61-year-old right-handed man with a history of metastatic adenocarcinoma of the colon. He had previously undergone resection of multiple lesions, Gamma Knife radiosurgery, and whole-brain radiation. Despite treatment of a left insular tumor, serial imaging revealed that the lesion continued to enlarge. Given the refractory nature of this tumor to radiation and the deep-seated location, the patient elected to undergo LITT treatment. The center of the lesion and entry point on the scalp were identified with STEALTH (Medtronic, Memphis, Tennessee) image-guided navigation. The AXiiiS Stereotactic Miniframe (Monteris Medical) for the LITT system was secured onto the skull, and a trajectory was defined to achieve access to the centroid of the tumor. After a burr hole was made, a gadolinium template probe was inserted into the AXiiiS base. The trajectory was confirmed via an intraoperative MRI, and the LITT probe driver was attached to the base and CO2-cooled, side-firing laser LITT probe. The laser was activated and thermometry images were obtained. Two trajectories, posteromedial and anterolateral, produced satisfactory tumor ablation. LITT with intraoperative MRI and stereotactic image guidance is a newly available, minimally invasive, and therapeutically viable technique for the treatment of deep seated brain tumors.

A comparative experimental approach to ecotoxicology in shallow-water and deep-sea holothurians suggests similar behavioural responses.

PubMed

Brown, Alastair; Wright, Roseanna; Mevenkamp, Lisa; Hauton, Chris

2017-10-01

Exploration of deep-sea mineral resources is burgeoning, raising concerns regarding ecotoxicological impacts on deep-sea fauna. Assessing toxicity in deep-sea species is technologically challenging, which promotes interest in establishing shallow-water ecotoxicological proxy species. However, the effects of temperature and hydrostatic pressure on toxicity, and how adaptation to deep-sea environmental conditions might moderate these effects, are unknown. To address these uncertainties we assessed behavioural and physiological (antioxidant enzyme activity) responses to exposure to copper-spiked artificial sediments in a laboratory experiment using a shallow-water holothurian (Holothuria forskali), and in an in situ experiment using a deep-sea holothurian (Amperima sp.). Both species demonstrated sustained avoidance behaviour, evading contact with contaminated artificial sediment. However, A. sp. demonstrated sustained avoidance of 5mgl -1 copper-contaminated artificial sediment whereas H. forskali demonstrated only temporary avoidance of 5mgl -1 copper-contaminated artificial sediment, suggesting that H. forskali may be more tolerant of metal exposure over 96h. Nonetheless, the acute behavioural response appears consistent between the shallow-water species and the deep-sea species, suggesting that H. forskali may be a suitable ecotoxicological proxy for A. sp. in acute (≤24h) exposures, which may be representative of deep-sea mining impacts. No antioxidant response was observed in either species, which was interpreted to be the consequence of avoiding copper exposure. Although these data suggest that shallow-water taxa may be suitable ecotoxicological proxies for deep-sea taxa, differences in methodological and analytical approaches, and in sex and reproductive stage of experimental subjects, require caution in assessing the suitability of H. forskali as an ecotoxicological proxy for A. sp. Nonetheless, avoidance behaviour may have bioenergetic consequences that affect growth and/or reproductive output, potentially impacting fecundity and/or offspring fitness, and thus influencing source-sink dynamics and persistence of wider deep-sea populations. Copyright © 2017 Elsevier B.V. All rights reserved.

Do different probing depths exhibit striking differences in microbial profiles?

PubMed

Pérez-Chaparro, Paula Juliana; McCulloch, John Anthony; Mamizuka, Elsa Masae; Moraes, Aline da Costa Lima; Faveri, Marcelo; Figueiredo, Luciene Cristina; Duarte, Poliana Mendes; Feres, Magda

2018-01-01

To perform a thorough characterization of the subgingival microbiota of shallow, moderate and deep sites in subjects with chronic periodontitis (ChP). Subgingival samples were collected from subjects with ChP (n = 3/category of probing depth: ≤3, 4-6 and ≥7 mm) and periodontal health (PH). Individual samples were submitted to 16S rDNA high- throughput sequencing and the analysis was made using mothur and R packages. Nine subjects with ChP and seven with PH were included and 101 samples were evaluated. Thirteen phyla, 118 genera and 211 OTUs were detected. Taxa from Chloroflexi and Spirochaetes phyla were associated with initial stages of disease. Fretibacterium, Eubacterium[XI][G-6], Desulfobulbus, Peptostreptococcaceae[XI][G-1] and [G-3], Bacteroidetes[G-3], Bacteroidaceae[G-1] genera and Filifactor alocis, Fretibacterium fastidiosum, Johnsonella spHOT166, Peptostreptococcaceae[XIII][G-1]HOT113, Porphyromonas endodontalis and Treponema sp. HOT258, which are not conventionally associated with disease, increased with the deepening of the pockets and/or were elevated in ChP; while Streptococcus, Corynebacterium and Bergeyella genera were associated with PH (p < .05). Striking differences were observed between the microbiota of shallow and moderate/deep sites, but not between moderate and deep sites in ChP subjects. Differences between shallow sites in PH and ChP were also observed. The characterized microbiota included known oral microorganisms and newly identified periodontal taxa, some of them not-yet-cultured. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Detection of resting state functional connectivity using partial correlation analysis: A study using multi-distance and whole-head probe near-infrared spectroscopy.

PubMed

Sakakibara, Eisuke; Homae, Fumitaka; Kawasaki, Shingo; Nishimura, Yukika; Takizawa, Ryu; Koike, Shinsuke; Kinoshita, Akihide; Sakurada, Hanako; Yamagishi, Mika; Nishimura, Fumichika; Yoshikawa, Akane; Inai, Aya; Nishioka, Masaki; Eriguchi, Yosuke; Matsuoka, Jun; Satomura, Yoshihiro; Okada, Naohiro; Kakiuchi, Chihiro; Araki, Tsuyoshi; Kan, Chiemi; Umeda, Maki; Shimazu, Akihito; Uga, Minako; Dan, Ippeita; Hashimoto, Hideki; Kawakami, Norito; Kasai, Kiyoto

2016-11-15

Multichannel near-infrared spectroscopy (NIRS) is a functional neuroimaging modality that enables easy-to-use and noninvasive measurement of changes in blood oxygenation levels. We developed a clinically-applicable method for estimating resting state functional connectivity (RSFC) with NIRS using a partial correlation analysis to reduce the influence of extraneural components. Using a multi-distance probe arrangement NIRS, we measured resting state brain activity for 8min in 17 healthy participants. Independent component analysis was used to extract shallow and deep signals from the original NIRS data. Pearson's correlation calculated from original signals was significantly higher than that calculated from deep signals, while partial correlation calculated from original signals was comparable to that calculated from deep (cerebral-tissue) signals alone. To further test the validity of our method, we also measured 8min of resting state brain activity using a whole-head NIRS arrangement consisting of 17 cortical regions in 80 healthy participants. Significant RSFC between neighboring, interhemispheric homologous, and some distant ipsilateral brain region pairs was revealed. Additionally, females exhibited higher RSFC between interhemispheric occipital region-pairs, in addition to higher connectivity between some ipsilateral pairs in the left hemisphere, when compared to males. The combined results of the two component experiments indicate that partial correlation analysis is effective in reducing the influence of extracerebral signals, and that NIRS is able to detect well-described resting state networks and sex-related differences in RSFC. Copyright © 2016 Elsevier Inc. All rights reserved.

Low-Temperature Spacecraft: Challenges/Opportunities

NASA Technical Reports Server (NTRS)

Dickman, J. E.; Patterson, R. L.; Overton, E.; Hammoud, A. N.; Gerber, S. S.

2001-01-01

Imagine sending a spacecraft into deep space that operates at the ambient temperature of its environment rather than hundreds of degrees Kelvin warmer. The average temperature of a spacecraft warmed only by the sun drops from 279 K near the Earth's orbit to 90 K near the orbit of Saturn, and to 44 K near Pluto's orbit. At present, deep space probes struggle to maintain an operating temperature near 300 K for the onboard electronics. To warm the electronics without consuming vast amounts of electrical energy, radioisotope heater units (RHUs) are used in vast numbers. Unfortunately, since RHU are always 'on', an active thermal management system is required to reject the excess heat. A spacecraft designed to operate at cryogenic temperatures and shielded from the sun by a large communication dish or solar cell array could be less complex, lighter, and cheaper than current deep space probes. Before a complete low-temperature spacecraft becomes a reality, there are several challenges to be met. Reliable cryogenic power electronics is one of the major challenges. The Low-Temperature Power Electronics Research Group at NASA Glenn Research Center (GRC) has demonstrated the ability of some commercial off the shelf power electronic components to operate at temperatures approaching that of liquid nitrogen (77 K). Below 77 K, there exists an opportunity for the development of reliable semiconductor power switching technologies other than bulk silicon CMOS. This paper will report on the results of NASA GRC's Low-Temperature Power Electronics Program and discuss the challenges to (opportunities for) the creation of a low-temperature spacecraft.

Reconfigurable visible nanophotonic switch for optogenetic applications (Conference Presentation)

NASA Astrophysics Data System (ADS)

Mohanty, Aseema; Li, Qian; Tadayon, Mohammad Amin; Bhatt, Gaurang R.; Cardenas, Jaime; Miller, Steven A.; Kepecs, Adam; Lipson, Michal

2017-02-01

High spatiotemporal resolution deep-brain optical excitation for optogenetics would enable activation of specific neural populations and in-depth study of neural circuits. Conventionally, a single fiber is used to flood light into a large area of the brain with limited resolution. The scalability of silicon photonics could enable neural excitation over large areas with single-cell resolution similar to electrical probes. However, active control of these optical circuits has yet to be demonstrated for optogenetics. Here we demonstrate the first active integrated optical switch for neural excitation at 473 nm, enabling control of multiple beams for deep-brain neural stimulation. Using a silicon nitride waveguide platform, we develop a cascaded Mach-Zehnder interferometer (MZI) network located outside the brain to direct light to 8 different grating emitters located at the tip of the neural probe. We use integrated platinum microheaters to induce a local thermo-optic phase shift in the MZI to control the switch output. We measure an ON/OFF extinction ratio of >8dB for a single switch and a switching speed of 20 microseconds. We characterize the optical output of the switch by imaging its excitation of fluorescent dye. Finally, we demonstrate in vivo single-neuron optical activation from different grating emitters using a fully packaged device inserted into a mouse brain. Directly activated neurons showed robust spike firing activities with low first-spike latency and small jitter. Active switching on a nanophotonic platform is necessary for eventually controlling highly-multiplexed reconfigurable optical circuits, enabling high-resolution optical stimulation in deep-brain regions.

The International Deep Planet Survey. II. The frequency of directly imaged giant exoplanets with stellar mass

NASA Astrophysics Data System (ADS)

Galicher, R.; Marois, C.; Macintosh, B.; Zuckerman, B.; Barman, T.; Konopacky, Q.; Song, I.; Patience, J.; Lafrenière, D.; Doyon, R.; Nielsen, E. L.

2016-10-01

Context. Radial velocity and transit methods are effective for the study of short orbital period exoplanets but they hardly probe objects at large separations for which direct imaging can be used. Aims: We carried out the international deep planet survey of 292 young nearby stars to search for giant exoplanets and determine their frequency. Methods: We developed a pipeline for a uniform processing of all the data that we have recorded with NIRC2/Keck II, NIRI/Gemini North, NICI/Gemini South, and NACO/VLT for 14 yr. The pipeline first applies cosmetic corrections and then reduces the speckle intensity to enhance the contrast in the images. Results: The main result of the international deep planet survey is the discovery of the HR 8799 exoplanets. We also detected 59 visual multiple systems including 16 new binary stars and 2 new triple stellar systems, as well as 2279 point-like sources. We used Monte Carlo simulations and the Bayesian theorem to determine that 1.05+2.80-0.70% of stars harbor at least one giant planet between 0.5 and 14 MJ and between 20 and 300 AU. This result is obtained assuming uniform distributions of planet masses and semi-major axes. If we consider power law distributions as measured for close-in planets instead, the derived frequency is 2.30+5.95-1.55%, recalling the strong impact of assumptions on Monte Carlo output distributions. We also find no evidence that the derived frequency depends on the mass of the hosting star, whereas it does for close-in planets. Conclusions: The international deep planet survey provides a database of confirmed background sources that may be useful for other exoplanet direct imaging surveys. It also puts new constraints on the number of stars with at least one giant planet reducing by a factor of two the frequencies derived by almost all previous works. Tables 11-15 are only available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (http://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/594/A63

49 CFR 572.200 - Instrumentation and test conditions.

Code of Federal Regulations, 2013 CFR

2013-10-01

... a minimum mass moment of inertia of 3646 kg-cm2. (b) The test probe for the lateral abdomen impact... and it has a minimum mass moment of inertia of 3646 kg-cm2. (c) The test probe for the pelvis-iliac... impact surface 50.8 × 88.9 mm for a depth of at least 76 mm and a minimum mass moment of inertia of 5000...

49 CFR 572.200 - Instrumentation and test conditions.

Code of Federal Regulations, 2014 CFR

2014-10-01

... a minimum mass moment of inertia of 3646 kg-cm2. (b) The test probe for the lateral abdomen impact... and it has a minimum mass moment of inertia of 3646 kg-cm2. (c) The test probe for the pelvis-iliac... impact surface 50.8 × 88.9 mm for a depth of at least 76 mm and a minimum mass moment of inertia of 5000...

Drop impact into a deep pool: vortex shedding and jet formation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Agbaglah, G.; Thoraval, M. -J.; Thoroddsen, S. T.

2015-02-01

One of the simplest splashing scenarios results from the impact of a single drop on a deep pool. The traditional understanding of this process is that the impact generates an axisymmetric sheet-like jet that later breaks up into secondary droplets. Recently it was shown that even this simplest of scenarios is more complicated than expected because multiple jets can be generated from a single impact event and there are transitions in the multiplicity of jets as the experimental parameters are varied. Here, we use experiments and numerical simulations of a single drop impacting on a deep pool to examine themore » transition from impacts that produce a single jet to those that produce two jets. Using high-speed X-ray imaging methods we show that vortex separation within the drop leads to the formation of a second jet long after the formation of the ejecta sheet. Using numerical simulations we develop a phase diagram for this transition and show that the capillary number is the most appropriate order parameter for the transition.« less

Understanding the Effects of Collisional Evolution and Spacecraft Impact Experiments on Comets and Asteroids

NASA Technical Reports Server (NTRS)

Lederer, S.M.; Jensen, E.A.; Fane, M.; Smith, D.C.; Holmes, J.; Keller, L.P.; Lindsay, S.S.; Wooden, D.H.; Whizin, A.; Cintala, M.J.;

Variations in collagen fibrils network structure and surface dehydration of acid demineralized intertubular dentin: effect of dentin depth and air-exposure time.

PubMed

Fawzy, Amr S

2010-01-01

The aim was to characterize the variations in the structure and surface dehydration of acid demineralized intertubular dentin collagen network with the variations in dentin depth and time of air-exposure (3, 6, 9 and 12 min). In addition, to study the effect of these variations on the tensile bond strength (TBS) to dentin. Phosphoric acid demineralized superficial and deep dentin specimens were prepared. The structure of the dentin collagen network was characterized by AFM. The surface dehydration was characterized by probing the nano-scale adhesion force (F(ad)) between AFM tip and intertubular dentin surface as a new experimental approach. The TBS to dentin was evaluated using an alcohol-based dentin self-priming adhesive. AFM images revealed a demineralized open collagen network structure in both of superficial and deep dentin at 3 and 6 min of air-exposure. However, at 9 min, superficial dentin showed more collapsed network structure compared to deep dentin that partially preserved the open network structure. Total collapsed structure was found at 12 min for both of superficial and deep dentin. The value of the F(ad) is decreased with increasing the time of air-exposure and is increased with dentin depth at the same time of air-exposure. The TBS was higher for superficial dentin at 3 and 6 min, however, no difference was found at 9 and 12 min. The ability of the demineralized dentin collagen network to resist air-dehydration and to preserve the integrity of open network structure with the increase in air-exposure time is increased with dentin depth. Although superficial dentin achieves higher bond strength values, the difference in the bond strength is decreased by increasing the time of air-exposure. The AFM probed F(ad) showed to be sensitive approach to characterize surface dehydration, however, further researches are recommended regarding the validity of such approach.

Education And Public Outreach For NASA's EPOXI Mission

NASA Astrophysics Data System (ADS)

McFadden, Lucy-Ann A.; Warner, E. M.; Crow, C. A.; Ristvey, J. D.; Counley, J.

2008-09-01

NASA's EPOXI mission has two scientific objectives in using the Deep Impact flyby spacecraft for further studies of comets and adding studies of extra-solar planets around other stars. During the Extrasolar Planetary Observations and Characterization (EPOCh) phase of the mission, observations of extrasolar planets transiting their parent stars are observed to further knowledge and understanding of planetary systems. Observations of Earth allow for comparison with Earth-like planets around other stars. A movie of Earth during a day when the Moon passed between Earth and the spacecraft is an educational highlight with scientific significance. The Deep Impact Extended Investigation (DIXI) continues the Deep Impact theme of investigating comets with a flyby of comet Hartley 2 in November 2010 to further explore the properties of comets and their formation. The EPOXI Education and Public Outreach (E/PO) program builds upon existing materials related to exploring comets and the Deep Impact mission, updating and modifying activities based on results from Deep Impact. An educational activity called Comparing Comets is under development that will guide students in conducting analyses similar to those that DIXI scientists will perform after observing comet Hartley 2. Existing educational materials related to planet finding from other NASA programs are linked from EPOXI's web page. Journey Through the Universe at the National Air and Space Museum encourages education in family and community groups and reaches out to underrepresented minorities. EPOXI's E/PO program additionally offers a newsletter to keep the public, teachers, and space enthusiasts apprised of mission activities. For more information visit: http://epoxi.umd.edu.

The photostability of the commonly used biotin-4-fluorescein probe.

PubMed

Haack, Richard A; Swift, Kerry M; Ruan, Qiaoqiao; Himmelsbach, Richard J; Tetin, Sergey Y

2017-08-15

Biotin-4-fluorescein (B4F) is a commonly used fluorescent probe for studying biotin-(strept)avidin interactions. During a characterization study of an anti-biotin antibody, using B4F as the probe, we noticed a discrepancy in the expected and experimentally determined number of biotin binding sites. Analytical testing showed that the biotin moiety in the probe undergoes a photosensitized oxidation to produce a mixture of biotin sulfoxides which has the potential to impact the quantitation of binding sites using this fluorescent probe. Copyright © 2017 Elsevier Inc. All rights reserved.

Two-Element Transducer for Ultrasound

NASA Technical Reports Server (NTRS)

Lecroissette, D. H.; Heyser, R. C.

1986-01-01

Separation of transmitting and receiving units improves probing of deep tissue. Ultrasonic transducer has dual elements to increase depth at which sonic images are made of biological tissue. Transducer uses separate transmitting and receiving elements, and frequency response of receiving element independently designed to accommodate attenuation of higher frequencies by tissue. New transducer intended for pulse-echo ultrasonic systems in which reflected sound pulses reveal features in tissue.

Expanding anchored hybrid enrichment to resolve both deep and shallow relationships within the spider tree of life.

PubMed

Hamilton, Chris A; Lemmon, Alan R; Lemmon, Emily Moriarty; Bond, Jason E

2016-10-13

Despite considerable effort, progress in spider molecular systematics has lagged behind many other comparable arthropod groups, thereby hindering family-level resolution, classification, and testing of important macroevolutionary hypotheses. Recently, alternative targeted sequence capture techniques have provided molecular systematics a powerful tool for resolving relationships across the Tree of Life. One of these approaches, Anchored Hybrid Enrichment (AHE), is designed to recover hundreds of unique orthologous loci from across the genome, for resolving both shallow and deep-scale evolutionary relationships within non-model systems. Herein we present a modification of the AHE approach that expands its use for application in spiders, with a particular emphasis on the infraorder Mygalomorphae. Our aim was to design a set of probes that effectively capture loci informative at a diversity of phylogenetic timescales. Following identification of putative arthropod-wide loci, we utilized homologous transcriptome sequences from 17 species across all spiders to identify exon boundaries. Conserved regions with variable flanking regions were then sought across the tick genome, three published araneomorph spider genomes, and raw genomic reads of two mygalomorph taxa. Following development of the 585 target loci in the Spider Probe Kit, we applied AHE across three taxonomic depths to evaluate performance: deep-level spider family relationships (33 taxa, 327 loci); family and generic relationships within the mygalomorph family Euctenizidae (25 taxa, 403 loci); and species relationships in the North American tarantula genus Aphonopelma (83 taxa, 581 loci). At the deepest level, all three major spider lineages (the Mesothelae, Mygalomorphae, and Araneomorphae) were supported with high bootstrap support. Strong support was also found throughout the Euctenizidae, including generic relationships within the family and species relationships within the genus Aptostichus. As in the Euctenizidae, virtually identical topologies were inferred with high support throughout Aphonopelma. The Spider Probe Kit, the first implementation of AHE methodology in Class Arachnida, holds great promise for gathering the types and quantities of molecular data needed to accelerate an understanding of the spider Tree of Life by providing a mechanism whereby different researchers can confidently and effectively use the same loci for independent projects, yet allowing synthesis of data across independent research groups.

Exploring Our Solar System with CubeSats and NanoSats

NASA Technical Reports Server (NTRS)

Freeman, Anthony; Norton, Charles

2015-01-01

The Jet Propulsion Laboratory (JPL) is NASA's lead center for robotic exploration of our solar system. We are known for our large, flagship missions, such as Voyager, which gave humanity its first close look at Jupiter and Saturn; and the Mars Rovers, which have excited millions worldwide with their daring landing exploits. Less familiar to those outside NASA may be our role in developing the Kepler mission, which has discovered more than 2000 planets around other stars; or the recently launched Soil Moisture Active Passive (SMAP) mission, one of many JPL Earth Science missions. A recent JPL initiative has emphasized low cost missions that use rapidly evolving technology developed for CubeSats and NanoSat s to explore our solar system. Costs are significantly lower (by one or two orders of magnitude) than for conventional JPL missions, and development time is also significantly shorter. At present 21 such CubeSat flight projects are under way at the laboratory with various partners : some in flight, some in development, some in advanced formulation. Four are planned as deep space missions. To succeed in exploring deep space CubeSat/NanoSat missions have to address several challenges: the more severe radiation environment, communications and navigation at a distance, propulsion, and packaging of instruments that can return valuable science into a compact volume/mass envelope. Instrument technologies, including cameras, magnetometers, spectrometers, radiometers, and even radars are undergoing miniaturization to fit on these smaller platforms. Other key technologies are being matured for smallsats and NanoSats in deep space, including micro -electric propulsion, compact radio (and optical) communications, and onboard data reduction. This paper will describe missions that utilize these developments including the first two deep space CubeSats (INSPIRE), planned for launch in 2017; the first pair of CubeSats to be sent to another planet (MARCO), manifested with the InSight Mars lander launch in March of 2016; a helicopter "drone" on Mars to extend the reach of future rovers; plans for a Lunar Flashlight mission to shine a light on the permanently shadowed craters of the Moon's poles; a Near Earth Asteroid CubeSat missio n; and a CubeSat constellation to demonstrate time series measurements of storm systems on Earth. From these beginnings, the potential for CubeSats and NanoSats to add to our knowledge of the solar system could easily grow exponentially. Imagine if every deep space mission carried one or more CubeSats that could operate independently (even for a brief period) on arrival at their target body. At only incremental additional cost, such spacecraft could go closer, probe deeper, and provide science measurements that we would not risk with the host spacecraft. This paper will describe examples including a NanoSat to probe the composition of Venus' atmosphere, impactors and close flybys of Europa, lunar probes, and soft landers for the moons of Mars. Low cost access to deep space also offers the potential for independent CubeSat/NanoSat missions - allowing us to characterize the population of near Earth asteroids for example, deploy a constellation around Venus, or take closer looks at the asteroid belt.

The Search for Sustainable Subsurface Habitats on Mars, and the Sampling of Impact Ejecta

NASA Astrophysics Data System (ADS)

Ivarsson, Magnus; Lindgren, Paula

2010-07-01

On Earth, the deep subsurface biosphere of both the oceanic and the continental crust is well known for surviving harsh conditions and environments characterized by high temperatures, high pressures, extreme pHs, and the absence of sunlight. The microorganisms of the terrestrial deep biosphere have an excellent capacity for adapting to changing geochemistry, as the alteration of the crust proceeds and the conditions of their habitats slowly change. Despite an almost complete isolation from surface conditions and the surface biosphere, the deep biosphere of the crustal rocks has endured over geologic time. This indicates that the deep biosphere is a self-sufficient system, independent of the global events that occur at the surface, such as impacts, glaciations, sea level fluctuations, and climate changes. With our sustainable terrestrial subsurface biosphere in mind, the subsurface on Mars has often been suggested as the most plausible place to search for fossil Martian life, or even present Martian life. Since the Martian surface is more or less sterile, subsurface settings are the only place on Mars where life could have been sustained over geologic time. To detect a deep biosphere in the Martian basement, drilling is a requirement. However, near future Mars sample return missions are limited by the mission's payload, which excludes heavy drilling equipment and restrict the missions to only dig the topmost meter of the Martian soil. Therefore, the sampling and analysis of Martian impact ejecta has been suggested as a way of accessing the deeper Martian subsurface without using heavy drilling equipment. Impact cratering is a natural geological process capable of excavating and exposing large amounts of rock material from great depths up to the surface. Several studies of terrestrial impact deposits show the preservation of pre-impact biosignatures, such as fossilized organisms and chemical biological markers. Therefore, if the Martian subsurface contains a record of life, it is reasonable to assume that biosignatures derived from the Martian subsurface could also be preserved in the Martian impact ejecta.

49 CFR 572.34 - Thorax.

Code of Federal Regulations, 2014 CFR

2014-10-01

... each of the drawings subtended therein. (b) When impacted by a test probe conforming to § 572.36(a) at... measured by the test probe and shall have a sternum displacement measured relative to spine of 2.68 inches.... 208 (§ 571.208 of this chapter). EC01AU91.166 (3) Place the longitudinal centerline of the test probe...

49 CFR 572.34 - Thorax.

Code of Federal Regulations, 2012 CFR

2012-10-01

... each of the drawings subtended therein. (b) When impacted by a test probe conforming to § 572.36(a) at... measured by the test probe and shall have a sternum displacement measured relative to spine of 2.68 inches.... 208 (§ 571.208 of this chapter). EC01AU91.166 (3) Place the longitudinal centerline of the test probe...

49 CFR 572.72 - Head assembly and test procedure.

Code of Federal Regulations, 2014 CFR

2014-10-01

... a test probe conforming to § 572.77(a)(1) at 7 feet per second (fps) according to the test procedure... the test probe so that its longitudinal center line is— (i) At the forehead at the point of orthogonal... and midsagittal planes passing through this point. (3) Impact the head with the test probe so that at...

49 CFR 572.136 - Knees and knee impact test procedure.

Code of Federal Regulations, 2012 CFR

2012-10-01

... as measured with the test probe-mounted accelerometer must be not less than 3450 N (776 lbf) and not... tibia may contact any exterior surface. (3) Align the test probe so that throughout its stroke and at... movement at the time of initial contact between the impactor and the knee. (5) The test probe velocity at...

49 CFR 572.136 - Knees and knee impact test procedure.

Code of Federal Regulations, 2010 CFR

2010-10-01

... as measured with the test probe-mounted accelerometer must be not less than 3450 N (776 lbf) and not... tibia may contact any exterior surface. (3) Align the test probe so that throughout its stroke and at... movement at the time of initial contact between the impactor and the knee. (5) The test probe velocity at...

49 CFR 572.136 - Knees and knee impact test procedure.

Code of Federal Regulations, 2014 CFR

2014-10-01

... as measured with the test probe-mounted accelerometer must be not less than 3450 N (776 lbf) and not... tibia may contact any exterior surface. (3) Align the test probe so that throughout its stroke and at... movement at the time of initial contact between the impactor and the knee. (5) The test probe velocity at...

49 CFR 572.72 - Head assembly and test procedure.

Code of Federal Regulations, 2012 CFR

2012-10-01

... a test probe conforming to § 572.77(a)(1) at 7 feet per second (fps) according to the test procedure... the test probe so that its longitudinal center line is— (i) At the forehead at the point of orthogonal... and midsagittal planes passing through this point. (3) Impact the head with the test probe so that at...