32 CFR Appendix C to Part 77 - DD Form 2581-1, Public and Community Service Organization Validation

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 1 2013-07-01 2013-07-01 false DD Form 2581-1, Public and Community Service Organization Validation C Appendix C to Part 77 National Defense Department of Defense OFFICE OF THE SECRETARY..., App. C Appendix C to Part 77—DD Form 2581-1, Public and Community Service Organization Validation...

32 CFR Appendix D to Part 113 - Sample DD Form 2654, “Involuntary Allotment Notice and Processing”

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 1 2010-07-01 2010-07-01 false Sample DD Form 2654, âInvoluntary Allotment Notice and Processingâ D Appendix D to Part 113 National Defense Department of Defense OFFICE OF THE..., App. D Appendix D to Part 113—Sample DD Form 2654, “Involuntary Allotment Notice and Processing...

32 CFR Appendix C to Part 77 - DD Form 2581-1, Public and Community Service Organization Validation

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 1 2014-07-01 2014-07-01 false DD Form 2581-1, Public and Community Service Organization Validation C Appendix C to Part 77 National Defense Department of Defense OFFICE OF THE SECRETARY..., App. C Appendix C to Part 77—DD Form 2581-1, Public and Community Service Organization Validation...

32 CFR Appendix C to Part 77 - DD Form 2581-1, Public and Community Service Organization Validation

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 1 2010-07-01 2010-07-01 false DD Form 2581-1, Public and Community Service Organization Validation C Appendix C to Part 77 National Defense Department of Defense OFFICE OF THE SECRETARY..., App. C Appendix C to Part 77—DD Form 2581-1, Public and Community Service Organization Validation...

32 CFR Appendix C to Part 77 - DD Form 2581-1, Public and Community Service Organization Validation

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 1 2011-07-01 2011-07-01 false DD Form 2581-1, Public and Community Service Organization Validation C Appendix C to Part 77 National Defense Department of Defense OFFICE OF THE SECRETARY..., App. C Appendix C to Part 77—DD Form 2581-1, Public and Community Service Organization Validation...

32 CFR Appendix C to Part 77 - DD Form 2581-1, Public and Community Service Organization Validation

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 1 2012-07-01 2012-07-01 false DD Form 2581-1, Public and Community Service Organization Validation C Appendix C to Part 77 National Defense Department of Defense OFFICE OF THE SECRETARY..., App. C Appendix C to Part 77—DD Form 2581-1, Public and Community Service Organization Validation...

32 CFR Appendix C to Part 286 - DD Form 2086, “Record of Freedom of Information (FOI) Processing Cost”

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 2 2010-07-01 2010-07-01 false DD Form 2086, âRecord of Freedom of Information (FOI) Processing Costâ C Appendix C to Part 286 National Defense Department of Defense (Continued... INFORMATION ACT PROGRAM REGULATION Pt. 286, App. C Appendix C to Part 286—DD Form 2086, “Record of Freedom of...

32 CFR Appendix B to Part 154 - Request Procedures

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 1 2011-07-01 2011-07-01 false Request Procedures B Appendix B to Part 154 National Defense Department of Defense OFFICE OF THE SECRETARY OF DEFENSE SECURITY DEPARTMENT OF DEFENSE... request is for an ENTNANC, an original only of the DD Form 398-2 and a completed DD Form 2280 (Armed...

32 CFR Appendix B to Part 154 - Request Procedures

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 1 2010-07-01 2010-07-01 false Request Procedures B Appendix B to Part 154 National Defense Department of Defense OFFICE OF THE SECRETARY OF DEFENSE SECURITY DEPARTMENT OF DEFENSE... request is for an ENTNANC, an original only of the DD Form 398-2 and a completed DD Form 2280 (Armed...

32 CFR Appendix A to Part 1290 - Preparation Guide for DD Form 1805, Violation Notice

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 6 2010-07-01 2010-07-01 false Preparation Guide for DD Form 1805, Violation Notice A Appendix A to Part 1290 National Defense Other Regulations Relating to National Defense DEFENSE LOGISTICS AGENCY MISCELLANEOUS PREPARING AND PROCESSING MINOR OFFENSES AND VIOLATION NOTICES REFERRED TO U.S...

32 CFR Appendix B to Part 77 - DD Form 2581, Operation Transition Employer Registration

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 1 2011-07-01 2011-07-01 false DD Form 2581, Operation Transition Employer Registration B Appendix B to Part 77 National Defense Department of Defense OFFICE OF THE SECRETARY OF DEFENSE PERSONNEL, MILITARY AND CIVILIAN PROGRAM TO ENCOURAGE PUBLIC AND COMMUNITY SERVICE Pt. 77, App. B Appendix B...

32 CFR Appendix B to Part 77 - DD Form 2581, Operation Transition Employer Registration

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 1 2013-07-01 2013-07-01 false DD Form 2581, Operation Transition Employer Registration B Appendix B to Part 77 National Defense Department of Defense OFFICE OF THE SECRETARY OF DEFENSE PERSONNEL, MILITARY AND CIVILIAN PROGRAM TO ENCOURAGE PUBLIC AND COMMUNITY SERVICE Pt. 77, App. B Appendix B...

32 CFR Appendix A to Part 1290 - Preparation Guide for DD Form 1805, Violation Notice

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 6 2012-07-01 2012-07-01 false Preparation Guide for DD Form 1805, Violation Notice A Appendix A to Part 1290 National Defense Other Regulations Relating to National Defense DEFENSE LOGISTICS AGENCY MISCELLANEOUS PREPARING AND PROCESSING MINOR OFFENSES AND VIOLATION NOTICES REFERRED TO U.S...

32 CFR Appendix A to Part 1290 - Preparation Guide for DD Form 1805, Violation Notice

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 6 2013-07-01 2013-07-01 false Preparation Guide for DD Form 1805, Violation Notice A Appendix A to Part 1290 National Defense Other Regulations Relating to National Defense DEFENSE LOGISTICS AGENCY MISCELLANEOUS PREPARING AND PROCESSING MINOR OFFENSES AND VIOLATION NOTICES REFERRED TO U.S...

32 CFR Appendix A to Part 1290 - Preparation Guide for DD Form 1805, Violation Notice

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 6 2014-07-01 2014-07-01 false Preparation Guide for DD Form 1805, Violation Notice A Appendix A to Part 1290 National Defense Other Regulations Relating to National Defense DEFENSE LOGISTICS AGENCY MISCELLANEOUS PREPARING AND PROCESSING MINOR OFFENSES AND VIOLATION NOTICES REFERRED TO U.S...

75 FR 68333 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; DFARS...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-05

... Supplement (DFARS) Appendix F, Material Inspection and Receiving Report; DD Form 250, DD Form 250c, DD form... Report; and DD Forms 250, 250c, and 250-1. The clause at DFARS 252.246-7000 is used in contracts that... the Government a material inspection and receiving report (DD Form 250) in a manner and to the extent...

32 CFR Appendix E to Part 286 - DD Form 2564, “Annual Report Freedom of Information Act”

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 2 2013-07-01 2013-07-01 false DD Form 2564, âAnnual Report Freedom of Information Actâ E Appendix E to Part 286 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) FREEDOM OF INFORMATION ACT PROGRAM DOD FREEDOM OF INFORMATION ACT PROGRAM...

32 CFR Appendix E to Part 286 - DD Form 2564, “Annual Report Freedom of Information Act”

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 2 2012-07-01 2012-07-01 false DD Form 2564, âAnnual Report Freedom of Information Actâ E Appendix E to Part 286 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) FREEDOM OF INFORMATION ACT PROGRAM DOD FREEDOM OF INFORMATION ACT PROGRAM...

32 CFR Appendix E to Part 286 - DD Form 2564, “Annual Report Freedom of Information Act”

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 2 2010-07-01 2010-07-01 false DD Form 2564, âAnnual Report Freedom of Information Actâ E Appendix E to Part 286 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) FREEDOM OF INFORMATION ACT PROGRAM DOD FREEDOM OF INFORMATION ACT PROGRAM...

32 CFR Appendix E to Part 286 - DD Form 2564, “Annual Report Freedom of Information Act”

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 2 2011-07-01 2011-07-01 false DD Form 2564, âAnnual Report Freedom of Information Actâ E Appendix E to Part 286 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) FREEDOM OF INFORMATION ACT PROGRAM DOD FREEDOM OF INFORMATION ACT PROGRAM...

32 CFR Appendix E to Part 286 - DD Form 2564, “Annual Report Freedom of Information Act”

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 2 2014-07-01 2014-07-01 false DD Form 2564, âAnnual Report Freedom of Information Actâ E Appendix E to Part 286 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) FREEDOM OF INFORMATION ACT PROGRAM DOD FREEDOM OF INFORMATION ACT PROGRAM...

32 CFR Appendix C to Part 113 - Sample DD Form 2653, “Involuntary Allotment Application”

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 1 2013-07-01 2013-07-01 false Sample DD Form 2653, âInvoluntary Allotment Applicationâ C Appendix C to Part 113 National Defense Department of Defense OFFICE OF THE SECRETARY OF DEFENSE PERSONNEL, MILITARY AND CIVILIAN INDEBTEDNESS PROCEDURES OF MILITARY PERSONNEL Pt. 113, App. C Appendix C to...

48 CFR 245.7101-2 - DD Form 1149, Requisition and Invoice Shipping Document.

Code of Federal Regulations, 2010 CFR

2010-10-01

... DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT GOVERNMENT PROPERTY Plant Clearance Forms 245.7101-2 DD Form 1149, Requisition and Invoice Shipping Document. Use for transfer and donation of contractor inventory. Donations of industrial plant equipment may be shipped via...

32 CFR Appendix D to Part 286 - DD Form 2086-1, “Record of Freedom of Information (FOI) Processing Cost for Technical Data”

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 2 2010-07-01 2010-07-01 false DD Form 2086-1, âRecord of Freedom of Information (FOI) Processing Cost for Technical Dataâ D Appendix D to Part 286 National Defense Department of... FREEDOM OF INFORMATION ACT PROGRAM REGULATION Pt. 286, App. D Appendix D to Part 286—DD Form 2086-1...

48 CFR 253.208-2 - DD Form 448-2, Acceptance of MIPR.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false DD Form 448-2, Acceptance... REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CLAUSES AND FORMS FORMS Prescription of Forms 253.208-2 DD Form 448-2, Acceptance of MIPR. Follow the procedures at PGI 253.208-2 for use of DD Form 448-2. [71 FR 39005...

32 CFR Appendix C to Part 45 - DD Form 215

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 1 2010-07-01 2010-07-01 false DD Form 215 C Appendix C to Part 45 National... CERTIFICATE OF RELEASE OR DISCHARGE FROM ACTIVE DUTY (DD FORM 214/5 SERIES) Pt. 45, App. C Appendix C to Part 45—DD Form 215 EC21OC91.050 ...

76 FR 72914 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Government...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-28

... Inventory Schedules; DD Form 1639, Scrap Warranty; DD Form 1640, Request for Plant Clearance; DD Form 1641... information collection on respondents, including the use of automated collection techniques or other forms of... Government property; [[Page 72915

32 CFR Appendix B to Part 45 - DD Form 214ws

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 1 2010-07-01 2010-07-01 false DD Form 214ws B Appendix B to Part 45 National... CERTIFICATE OF RELEASE OR DISCHARGE FROM ACTIVE DUTY (DD FORM 214/5 SERIES) Pt. 45, App. B Appendix B to Part 45—DD Form 214ws EC23OC91.007 ...

48 CFR 247.371 - DD Form 1653, Transportation Data for Solicitations.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false DD Form 1653, Transportation Data for Solicitations. 247.371 Section 247.371 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT TRANSPORTATION Transportation in...

48 CFR 245.7101-4 - DD Form 1640, Request for Plant Clearance.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false DD Form 1640, Request for Plant Clearance. 245.7101-4 Section 245.7101-4 Federal Acquisition Regulations System DEFENSE... Forms 245.7101-4 DD Form 1640, Request for Plant Clearance. Use to request plant clearance assistance or...

77 FR 30998 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Government...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-24

... of Inventory Schedules; DD Form 1639, Scrap Warranty; DD Form 1640, Request for Plant Clearance; DD... respondents, including the use of automated collection techniques or other forms of information technology... contractor inventory. a. DFARS 211.274, Item identification and valuation requirements, and the associated...

76 FR 21851 - Defense Federal Acquisition Regulation Supplement; Construction and Architect-Engineer Services...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-19

... data on DD Form 2626, Performance Evaluation (Construction), and DD Form 2631, Performance Evaluation...(e) for the requirements for preparing past performance evaluations. The objective of this rule is to remove the requirement to use DD Forms 2626, Performance Evaluation (Construction), and 2631, Performance...

MEDBASE: Strategic Planning and Implementation of an Army Medical Department Software Application

DTIC Science & Technology

2003-07-17

the application. These forms include Department of Defense (DD) Forms 2795, 2796, a more comprehensive version of DD Form 2766, the medical and dental ...include DD Forms 2795, 2796, a more comprehensive version of DD Form 2766, the medical and dental readiness portions of DA Form 7425, and an expanded...Readiness •Mobilization •Aggregation CDA • Dental Readiness TAPDB •Conglomerate Personnel Information MEBITT •Medical Boards •Perm Profiles VISION •Eye

32 CFR 634.21 - Department of Defense Form 2220.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 4 2014-07-01 2013-07-01 true Department of Defense Form 2220. 634.21 Section 634.21 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) LAW... Department of Defense Form 2220. (a) Use. DD Form 2220 will be used to identify registered POVs on Army, Navy...

32 CFR 634.21 - Department of Defense Form 2220.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 4 2012-07-01 2011-07-01 true Department of Defense Form 2220. 634.21 Section 634.21 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) LAW... Department of Defense Form 2220. (a) Use. DD Form 2220 will be used to identify registered POVs on Army, Navy...

32 CFR 634.21 - Department of Defense Form 2220.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 4 2013-07-01 2013-07-01 false Department of Defense Form 2220. 634.21 Section 634.21 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) LAW... Department of Defense Form 2220. (a) Use. DD Form 2220 will be used to identify registered POVs on Army, Navy...

32 CFR 634.21 - Department of Defense Form 2220.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 4 2010-07-01 2010-07-01 true Department of Defense Form 2220. 634.21 Section 634.21 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) LAW... Department of Defense Form 2220. (a) Use. DD Form 2220 will be used to identify registered POVs on Army, Navy...

32 CFR 634.21 - Department of Defense Form 2220.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 4 2011-07-01 2011-07-01 false Department of Defense Form 2220. 634.21 Section 634.21 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) LAW... Department of Defense Form 2220. (a) Use. DD Form 2220 will be used to identify registered POVs on Army, Navy...

32 CFR Appendix A to Part 45 - DD Form 214

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 1 2010-07-01 2010-07-01 false DD Form 214 A Appendix A to Part 45 National... CERTIFICATE OF RELEASE OR DISCHARGE FROM ACTIVE DUTY (DD FORM 214/5 SERIES) Pt. 45, App. A Appendix A to Part 45—DD Form 214 EC23OC91.003 EC23OC91.004 EC23OC91.005 EC23OC91.006 [54 FR 9985, Mar. 9, 1989] ...

Defense Transportation Regulation Part 2, Cargo Movement.

DTIC Science & Technology

1998-08-01

Stop-Off Unloading Point (DD Form 1371) 202-18 202-7 Route Order Amendment (DD Form 2017 ) 202-19 204-1 Hazardous Materials Classification...DRO/IRO/SRO amendments will be submitted to the appropriate MTMC routing authority or theater CINC using DD Form 2017 , Route Order Amendment...Figure 202-5. 7. An example of a DD Form 2017 , Route Order Amendment, is provided in Figure 202.7. D. EXCEPTION Exceptions to assignment of routing

48 CFR 253.208-1 - DD Form 448, Military Interdepartmental Purchase Request.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false DD Form 448, Military Interdepartmental Purchase Request. 253.208-1 Section 253.208-1 Federal Acquisition Regulations System DEFENSE... DD Form 448, Military Interdepartmental Purchase Request. Follow the procedures at PGI 253.208-1 for...

32 CFR Appendix A to Part 1290 - Preparation Guide for DD Form 1805, Violation Notice

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 6 2011-07-01 2011-07-01 false Preparation Guide for DD Form 1805, Violation.... DISTRICT COURTS Pt. 1290, App. A Appendix A to Part 1290—Preparation Guide for DD Form 1805, Violation... data, as appropriate, and the place of court (i.e., Magistrate Court Address), the date and time of...

48 CFR 245.7206 - Transmitting DD Form 1342, DoD Property Record.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Instructions 245.7206 Transmitting DD Form 1342, DoD Property Record. As a minimum, the plant clearance officer... contractor acquired the equipment; (g) Location of the industrial plant equipment; (h) Total acquisition cost..., DoD Property Record. 245.7206 Section 245.7206 Federal Acquisition Regulations System DEFENSE...

75 FR 45104 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-02

... DEPARTMENT OF DEFENSE Defense Acquisition Regulations System [OMB Control Number 0704-0225] Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Administrative Matters... Supplement (DFARS) Part 204, Administrative Matters, and related clauses at DFARS 252.204; DD Form 2051...

32 CFR 65.8 - Reporting requirements.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 1 2012-07-01 2012-07-01 false Reporting requirements. 65.8 Section 65.8 National Defense Department of Defense OFFICE OF THE SECRETARY OF DEFENSE PERSONNEL, MILITARY AND CIVILIAN... assigned Report Control Symbols DD-P&R(AR)1221, DD-P&R(Q)2077, DD-RA(M)1147, DD-RA(D)1148, DD-RA(D)2170, DD...

75 FR 71560 - Defense Federal Acquisition Regulation Supplement; Cost and Software Data Reporting System (DFARS...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-24

... DEPARTMENT OF DEFENSE Defense Acquisition Regulations System 48 CFR Parts 215, 234, 242, and 252... System (DFARS Case 2008-D027) AGENCY: Defense Acquisition Regulations System, Department of Defense (DoD... 1921-2, Progress Curve Report Comment: A respondent noted that DD Form 1921-2 Progress Curve Report...

78 FR 26622 - Submission for OMB Review; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-07

... DEPARTMENT OF DEFENSE Office of the Secretary [Docket No. DoD-2013-OS-0018] Submission for OMB Review; Comment Request ACTION: Notice. The Department of Defense has submitted to OMB for clearance, the..., Associated Forms and OMB Number: Vietnam War Commemoration Planned Commemorative Events; DD Form 2956; OMB...

78 FR 26621 - Submission for OMB Review; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-07

... DEPARTMENT OF DEFENSE Office of the Secretary [Docket No. DoD-2013-OS-0017] Submission for OMB Review; Comment Request ACTION: Notice. The Department of Defense has submitted to OMB for clearance, the..., Associated Forms and OMB Number: Vietnam War Commemoration After-Action Report; DD Form 2957; OMB Number 0704...

32 CFR 750.6 - Claims: Presentment.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 5 2013-07-01 2013-07-01 false Claims: Presentment. 750.6 Section 750.6 National Defense Department of Defense (Continued) DEPARTMENT OF THE NAVY CLAIMS GENERAL CLAIMS REGULATIONS... Claims Act shall be submitted on DD Form 1842. 2 The claim and all other papers requiring the signature...

32 CFR 750.6 - Claims: Presentment.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 5 2010-07-01 2010-07-01 false Claims: Presentment. 750.6 Section 750.6 National Defense Department of Defense (Continued) DEPARTMENT OF THE NAVY CLAIMS GENERAL CLAIMS REGULATIONS... Claims Act shall be submitted on DD Form 1842. 2 The claim and all other papers requiring the signature...

32 CFR 750.6 - Claims: Presentment.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 5 2011-07-01 2011-07-01 false Claims: Presentment. 750.6 Section 750.6 National Defense Department of Defense (Continued) DEPARTMENT OF THE NAVY CLAIMS GENERAL CLAIMS REGULATIONS... Claims Act shall be submitted on DD Form 1842. 2 The claim and all other papers requiring the signature...

32 CFR 750.6 - Claims: Presentment.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 5 2012-07-01 2012-07-01 false Claims: Presentment. 750.6 Section 750.6 National Defense Department of Defense (Continued) DEPARTMENT OF THE NAVY CLAIMS GENERAL CLAIMS REGULATIONS... Claims Act shall be submitted on DD Form 1842. 2 The claim and all other papers requiring the signature...

32 CFR 855.8 - Application procedures.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 6 2013-07-01 2013-07-01 false Application procedures. 855.8 Section 855.8 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE AIRCRAFT CIVIL AIRCRAFT USE.... To allow time for processing, the application (DD Forms 2400, 2401, and 2402) and a self-addressed...

32 CFR 855.8 - Application procedures.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 6 2012-07-01 2012-07-01 false Application procedures. 855.8 Section 855.8 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE AIRCRAFT CIVIL AIRCRAFT USE.... To allow time for processing, the application (DD Forms 2400, 2401, and 2402) and a self-addressed...

32 CFR 855.8 - Application procedures.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 6 2014-07-01 2014-07-01 false Application procedures. 855.8 Section 855.8 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE AIRCRAFT CIVIL AIRCRAFT USE.... To allow time for processing, the application (DD Forms 2400, 2401, and 2402) and a self-addressed...

32 CFR 855.8 - Application procedures.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 6 2011-07-01 2011-07-01 false Application procedures. 855.8 Section 855.8 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE AIRCRAFT CIVIL AIRCRAFT USE.... To allow time for processing, the application (DD Forms 2400, 2401, and 2402) and a self-addressed...

32 CFR 842.24 - Filing a claim.

Code of Federal Regulations, 2010 CFR

2010-07-01

... properly completed AF Form 180, DD Form 1842 or other written and signed demand for a specified sum of money. (b) Amending a claim. A claimant may amend a claim at any time prior to the expiration of the... Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE CLAIMS AND LITIGATION ADMINISTRATIVE...

Ballistic Missile Defense in the European Theater: Political, Military and Technical Considerations

DTIC Science & Technology

2007-04-15

currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. 1. REPORT DATE (DD-MM-YYYY) 15 04 2007 2. REPORT TYPE...the European Security Strategy. These strategic documents implicitly and explicitly build a strong case for bolstering missile defense capabilities...states. The NMS advocates the building of a defense in depth by extending defensive capabilities well beyond United States borders, and uses the

32 CFR 253.6 - Procedures.

Code of Federal Regulations, 2010 CFR

2010-07-01

... continuation of assignment is not clearly consistent with the national interest. (d) Completed security forms (DD Form 398, Personnel Security Questionnaire (BI/SBI), or 398-2, Personnel Security Questionnaire (National Agency Check)) shall be forwarded to the Defense Industrial Security Clearance Office (DISCO...

32 CFR 169a.8 - Inventory and review schedule (Report Control Symbol DD-P&L(A)).

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 1 2011-07-01 2011-07-01 false Inventory and review schedule (Report Control Symbol DD-P&L(A)). 169a.8 Section 169a.8 National Defense Department of Defense OFFICE OF THE SECRETARY OF DEFENSE DEFENSE CONTRACTING COMMERCIAL ACTIVITIES PROGRAM PROCEDURES Procedures § 169a.8 Inventory...

32 CFR 169a.8 - Inventory and review schedule (Report Control Symbol DD-P&L(A)).

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 1 2013-07-01 2013-07-01 false Inventory and review schedule (Report Control Symbol DD-P&L(A)). 169a.8 Section 169a.8 National Defense Department of Defense OFFICE OF THE SECRETARY OF DEFENSE DEFENSE CONTRACTING COMMERCIAL ACTIVITIES PROGRAM PROCEDURES Procedures § 169a.8 Inventory...

32 CFR 169a.8 - Inventory and review schedule (Report Control Symbol DD-P&L(A)).

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 1 2010-07-01 2010-07-01 false Inventory and review schedule (Report Control Symbol DD-P&L(A)). 169a.8 Section 169a.8 National Defense Department of Defense OFFICE OF THE SECRETARY OF DEFENSE DEFENSE CONTRACTING COMMERCIAL ACTIVITIES PROGRAM PROCEDURES Procedures § 169a.8 Inventory...

77 FR 11494 - Proposed Collection; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-27

... Defense Pentagon, Washington, DC 20301-4000 or call (703) 588-0877. Title, Associated Form, and OMB... Certified to Have Performed Active Duty with the Armed Forces of the United States, DD Form 2168, OMB... the United States by certain groups shall be considered Active Duty service, and (2) to award members...

78 FR 52168 - Submission for OMB Review; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-22

... Review; Comment Request ACTION: Notice. The Department of Defense has submitted to OMB for clearance, the.... Title, Associated Form and OMB Number: Request for Armed Forces Participation in Public Events; DD Form... evaluate the eligibility of events to receive Armed Forces community relations support and to determine...

Defense Depot Mechanicsburg Total Quality Management Implementation Plan

DTIC Science & Technology

1989-06-01

B T I TLEE 5 . FUNDING NUMBERS Defense Depot Mechanicsburg Total Quality Management Implementation Plan 6. AUTHOR(S) 7. PERFORMING ORGANIZATION NAME...Form 298 (Rev. 2-89) L296- 102 Acces.ion For NYI J ... I:: ted DEFENSE DEPOT MECHANICSBURG PENNSYLVANIAL--I By_ TOTAL QUALITY MANAGEMENT K_~ t buty-n...IMPLEMENTATION PLAN Avmail-t!Ilty Codes IvLl c 2Dd/or JUN 3 0 1989 iDizt Special PURPOSE The purpose of this Total Quality Management Implementation

Tomato SlMKK2 and SlMKK4 contribute to disease resistance against Botrytis cinerea

PubMed Central

2014-01-01

Background Mitogen-activated protein kinase (MAPK) cascades are highly conserved signaling modules that mediate the transduction of extracellular stimuli via receptors/sensors into intracellular responses and play key roles in plant immunity against pathogen attack. However, the function of tomato MAPK kinases, SlMKKs, in resistance against Botrytis cinerea remains unclear yet. Results A total of five SlMKK genes with one new member, SlMKK5, were identified in tomato. qRT-PCR analyses revealed that expression of SlMKK2 and SlMKK4 was strongly induced by B. cinerea and by jasmonic acid and ethylene precursor 1-amino cyclopropane-1-carboxylic acid. Virus-induced gene silencing (VIGS)-based knockdown of individual SlMKKs and disease assays identified that SlMKK2 and SlMKK4 but not other three SlMKKs (SlMKK1, SlMKK3 and SlMKK5) are involved in resistance against B. cinerea. Silencing of SlMKK2 or SlMKK4 resulted in reduced resistance to B. cinerea, increased accumulation of reactive oxygen species and attenuated expression of defense genes after infection of B. cinerea in tomato plants. Furthermore, transient expression of constitutively active phosphomimicking forms SlMKK2DD and SlMKK4DD in leaves of Nicotiana benthamiana plants led to enhanced resistance to B. cinerea and elevated expression of defense genes. Conclusions VIGS-based knockdown of SlMKK2 and SlMKK4 expression in tomato and gain-of-function transient expression of constitutively active phosphomimicking forms SlMKK2DD and SlMKK2DD in N. benthamiana demonstrate that both SlMKK2 and SlMKK4 function as positive regulators of defense response against B. cinerea. PMID:24930014

32 CFR 728.33 - Nonavailability statement (DD 1251).

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 5 2012-07-01 2012-07-01 false Nonavailability statement (DD 1251). 728.33 Section 728.33 National Defense Department of Defense (Continued) DEPARTMENT OF THE NAVY PERSONNEL MEDICAL AND DENTAL CARE FOR ELIGIBLE PERSONS AT NAVY MEDICAL DEPARTMENT FACILITIES Retired Members and...

75 FR 56961 - Defense Federal Acquisition Regulation Supplement; Material Inspection and Receiving Report...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-17

...DoD is issuing a proposed rule to update Defense Federal Acquisition Regulation Supplement (DFARS), Appendix F, Material Inspection and Receiving Report, to incorporate procedures for using the electronic Wide Area Workflow Receiving Report required for use in most contracts in lieu of the DD Form 250, Material Inspection and Receiving Report, which is now used mostly on an exception basis.

77 FR 3235 - Proposed Collection; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-23

... Collection The DD Form 370 is a form in which an applicant, who requests a waiver to enter the Armed Forces... eligibility standards to become a member of the Armed Forces of the United States. Dated: January 17, 2012... DEPARTMENT OF DEFENSE Office of the Secretary [Docket ID: DOD-2012-OS-0006] Proposed Collection...

Functions of the Department of Defense and Its Major Components

DTIC Science & Technology

2010-12-21

Information Center (DTIC),” August 19, 2005 (ax) DoD Directive 5105.72, “Defense Technology Security Administration ( DTSA ),” July 28, 2005 (ay) DoD...Information Center (DTIC). See DoDD 5105.73 (Reference (aw)). (4) Defense Technology Security Administration ( DTSA ). See DoDD 5105.72

77 FR 63296 - Proposed Collection; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-16

... Finance and Accounting Service announces the proposed public information collection and seeks public... Accounting Service (DFAS). The information from the DD Form X602 is used by DFAS in processing the applicant...; Comment Request AGENCY: Defense Finance and Accounting Service, DoD. [[Page 63297

32 CFR 643.126 - Transportation licenses.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 4 2010-07-01 2010-07-01 true Transportation licenses. 643.126 Section 643.126... ESTATE Additional Authority of Commanders § 643.126 Transportation licenses. Installation commanders are... free competitive proposals of all available companies or individuals. (b) DD Form 694 (Transportation...

32 CFR 643.126 - Transportation licenses.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 4 2011-07-01 2011-07-01 false Transportation licenses. 643.126 Section 643.126... ESTATE Additional Authority of Commanders § 643.126 Transportation licenses. Installation commanders are... free competitive proposals of all available companies or individuals. (b) DD Form 694 (Transportation...

32 CFR 643.126 - Transportation licenses.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 4 2012-07-01 2011-07-01 true Transportation licenses. 643.126 Section 643.126... ESTATE Additional Authority of Commanders § 643.126 Transportation licenses. Installation commanders are... free competitive proposals of all available companies or individuals. (b) DD Form 694 (Transportation...

32 CFR 643.126 - Transportation licenses.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 4 2013-07-01 2013-07-01 false Transportation licenses. 643.126 Section 643.126... ESTATE Additional Authority of Commanders § 643.126 Transportation licenses. Installation commanders are... free competitive proposals of all available companies or individuals. (b) DD Form 694 (Transportation...

32 CFR 643.126 - Transportation licenses.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 4 2014-07-01 2013-07-01 true Transportation licenses. 643.126 Section 643.126... ESTATE Additional Authority of Commanders § 643.126 Transportation licenses. Installation commanders are... free competitive proposals of all available companies or individuals. (b) DD Form 694 (Transportation...

48 CFR 237.7001 - Method of acquisition.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Method of acquisition. 237.7001 Section 237.7001 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM... contract exists, use DD Form 1155, Order for Supplies or Services, to obtain mortuary services. ...

48 CFR 216.505 - Ordering.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Ordering. 216.505 Section 216.505 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF... issued on DD Form 1155, Order for Supplies or Services. [63 FR 11529, Mar. 9, 1998, as amended at 70 FR...

48 CFR 204.7204 - Maintenance of the CAGE file.

Code of Federal Regulations, 2010 CFR

2010-10-01

...) Submit requests for changes to CAGE files on DD Form 2051, or electronic equivalent, to—Defense Logistics Information Service, DLIS-SBB, Federal Center, 74 Washington Avenue, North, Battle Creek, MI 49017-3084... codes is in Volume 7 of DoD 4100.39-M, Federal Logistics Information System (FLIS) Procedures Manual...

78 FR 71677 - Excepted Service

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-29

... Office of Public Press Assistant.... DC130098 10/21/2013 Affairs. Office of Under Director of DC130102 10..... DD130129 10/17/2013 Secretary of Defense (Legislative Affairs). Office of Assistant Senior Public DD140003 10/31/2013 Secretary of Affairs Advisor. Defense (Public Affairs). DEPARTMENT OF EDUCATION Office of...

LDDX: A High Efficiency Air Conditioner for DOD Buildings

DTIC Science & Technology

2017-02-01

Defense. Page Intentionally Left Blank REPORT DOCUMENTATION PAGE Form Approved OMB No. 0704-0188 Public reporting burden for this collection of...information if it does not display a currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. 1. REPORT DATE (DD-MM...OF RESPONSIBLE PERSON a. REPORT b. ABSTRACT c. THIS PAGE 93 19b. TELEPHONE NUMBER (include area code) Standard Form 298 (Rev. 8-98) Prescribed by

41 CFR 101-30.503 - Maintenance actions required.

Code of Federal Regulations, 2011 CFR

2011-07-01

..., the agency shall submit data to GSA, the Defense Logistics Agency (DLA), the Veterans Administration... submission to DLSC, except that civilian agencies receiving supply support on an item from a DLA center or... DLA center using DD Form 1685, Data Exchange and/or Proposed Revision of Catalog Data, or to the VA...

41 CFR 101-30.503 - Maintenance actions required.

Code of Federal Regulations, 2013 CFR

2013-07-01

..., the agency shall submit data to GSA, the Defense Logistics Agency (DLA), the Veterans Administration... submission to DLSC, except that civilian agencies receiving supply support on an item from a DLA center or... DLA center using DD Form 1685, Data Exchange and/or Proposed Revision of Catalog Data, or to the VA...

41 CFR 101-30.503 - Maintenance actions required.

Code of Federal Regulations, 2012 CFR

2012-07-01

..., the agency shall submit data to GSA, the Defense Logistics Agency (DLA), the Veterans Administration... submission to DLSC, except that civilian agencies receiving supply support on an item from a DLA center or... DLA center using DD Form 1685, Data Exchange and/or Proposed Revision of Catalog Data, or to the VA...

41 CFR 101-30.503 - Maintenance actions required.

Code of Federal Regulations, 2014 CFR

2014-07-01

..., the agency shall submit data to GSA, the Defense Logistics Agency (DLA), the Veterans Administration... submission to DLSC, except that civilian agencies receiving supply support on an item from a DLA center or... DLA center using DD Form 1685, Data Exchange and/or Proposed Revision of Catalog Data, or to the VA...

Obtaining Life-Cycle Cost-Effective Facilities in the Department of Defense

DTIC Science & Technology

2013-01-01

8 Step 3: Regional, Service- Level , and OSD Project Ranking...13 2.3. Actors and Barriers to Life-Cycle Cost-Effective Facilities in the Regional, Service- Level , and OSD Project Ranking...Congressional authorization and appropriation OMB evaluation Regional, service- level , and OSD project ranking Economic analysis and DD form 1391 completed

Coupling Geothermal Heat Pumps with Underground Seasonal Thermal Energy Storage

DTIC Science & Technology

2017-03-21

endorsement, recommendation, or favoring by the Department of Defense. Page Intentionally Left Blank REPORT DOCUMENTATION PAGE Form Approved OMB No...RETURN YOUR FORM TO THE ABOVE ADDRESS. 1. REPORT DATE (DD-MM-YYYY) 03-21-2017 2. REPORT TYPE Cost & Performance Report 3. DATES COVERED (From - To...c. THIS PAGE U U 91 19b. TELEPHONE NUMBER (include area code) 478-405-8301 Standard Form 298 (Rev. 8-98) Prescribed by ANSI Std. Z39.18

Sustainable, Green Vinyl Ester Resin (VER) from Renewable Resources

DTIC Science & Technology

2017-04-02

its endorsement, recommendation, or favoring by the Department of Defense. Page Intentionally Left Blank REPORT DOCUMENTATION PAGE Form Approved OMB...RETURN YOUR FORM TO THE ABOVE ADDRESS. 1. REPORT DATE (DD-MM-YYYY) 04-02-2017 2. REPORT TYPE Final Report 3. DATES COVERED (From - To) 09/2013-06...Yongwoo Lee a. REPORT b. ABSTRACT c. THIS PAGE 798 19b. TELEPHONE NUMBER (include area code) 978-934-3650 Standard Form 298 (Rev. 8-98

Coupling Geothermal Heat Pumps with Underground Seasonal Thermal Energy Storage (EW-201135)

DTIC Science & Technology

2017-03-01

recommendation, or favoring by the Department of Defense. Page Intentionally Left Blank REPORT DOCUMENTATION PAGE Form Approved OMB No. 0704... FORM TO THE ABOVE ADDRESS. 1. REPORT DATE (DD-MM-YYYY) 03-21-2017 2. REPORT TYPE Final Report 3. DATES COVERED (From - To) 09/2011-03/2017 4...91 19b. TELEPHONE NUMBER (include area code) 478-405-8301 Standard Form 298 (Rev. 8-98) Prescribed by ANSI Std. Z39.18 Page

DDG 1000 Zumwalt Class Destroyer (DDG 1000)

DTIC Science & Technology

2013-12-01

Missile Defense Radar is the most cost-effective solution to fleet air and missile defense requirements. The Secretary of the Navy notified Congress...not reach an affordable solution and deliveries of these components for DDG 1002 were becoming time-critical. The Navy concurrently pursued a steel...DD(X) Construction (Shared) (Sunk) 2464 DD(X) Sys Design, Dev & Integration (Shared) (Sunk) 2465 DC Survivability (Shared) (Sunk) 2466 MFR

48 CFR 211.602 - General.

Code of Federal Regulations, 2010 CFR

2010-10-01

... DEFENSE ACQUISITION PLANNING DESCRIBING AGENCY NEEDS Priorities and Allocations 211.602 General. DoD implementation of the Defense Priorities and Allocations System is in DoDD 4400.1, Defense Production Act...

48 CFR 211.602 - General.

Code of Federal Regulations, 2012 CFR

2012-10-01

... DEFENSE ACQUISITION PLANNING DESCRIBING AGENCY NEEDS Priorities and Allocations 211.602 General. DoD implementation of the Defense Priorities and Allocations System is in DoDD 4400.1, Defense Production Act...

48 CFR 211.602 - General.

Code of Federal Regulations, 2013 CFR

2013-10-01

... DEFENSE ACQUISITION PLANNING DESCRIBING AGENCY NEEDS Priorities and Allocations 211.602 General. DoD implementation of the Defense Priorities and Allocations System is in DoDD 4400.1, Defense Production Act...

48 CFR 211.602 - General.

Code of Federal Regulations, 2011 CFR

2011-10-01

... DEFENSE ACQUISITION PLANNING DESCRIBING AGENCY NEEDS Priorities and Allocations 211.602 General. DoD implementation of the Defense Priorities and Allocations System is in DoDD 4400.1, Defense Production Act...

48 CFR 211.602 - General.

Code of Federal Regulations, 2014 CFR

2014-10-01

... DEFENSE ACQUISITION PLANNING DESCRIBING AGENCY NEEDS Priorities and Allocations 211.602 General. DoD implementation of the Defense Priorities and Allocations System is in DoDD 4400.1, Defense Production Act...

Army Logistician. Volume 38, Issue 4, July-August 2006

DTIC Science & Technology

2006-08-01

Relationships Effective joint logistics depends on clear roles, accountabilities , and relationships among the global players within the joint logistics...well-understood roles and accountabilities of the players involved in those processes, and shared JFC metrics shape this enabler. Domain-wide...DD [Department of Defense] Form 10) (when applicable) (for sensitive cargo accountability ) X X X UIC and shipment unit number (stenciled) X (4

32 CFR 103.1 - Purpose.

Code of Federal Regulations, 2014 CFR

2014-07-01

.... Department of Defense paper, “The Department of Defense Sexual Assault Prevention Strategy,” September 30... Department of Defense OFFICE OF THE SECRETARY OF DEFENSE PERSONNEL, MILITARY AND CIVILIAN SEXUAL ASSAULT.../302041p.pdf; (8) U.S. Department of Defense, “Manual for Courts-Martial,” 2008; (9) DoDD 7050.06...

32 CFR 103.1 - Purpose.

Code of Federal Regulations, 2013 CFR

2013-07-01

.... Department of Defense paper, “The Department of Defense Sexual Assault Prevention Strategy,” September 30... Department of Defense OFFICE OF THE SECRETARY OF DEFENSE PERSONNEL, MILITARY AND CIVILIAN SEXUAL ASSAULT.../302041p.pdf; (8) U.S. Department of Defense, “Manual for Courts-Martial,” 2008; (9) DoDD 7050.06...

32 CFR 310.7 - Information requirements.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 2 2014-07-01 2014-07-01 false Information requirements. 310.7 Section 310.7 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) PRIVACY... § 310.6(d)(7) are assigned Report Control Symbol DD-DA&M(A)1379. ...

32 CFR 310.7 - Information requirements.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 2 2010-07-01 2010-07-01 false Information requirements. 310.7 Section 310.7 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) PRIVACY... § 310.6(d)(7) are assigned Report Control Symbol DD-DA&M(A)1379. ...

32 CFR 310.7 - Information requirements.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 2 2011-07-01 2011-07-01 false Information requirements. 310.7 Section 310.7 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) PRIVACY... § 310.6(d)(7) are assigned Report Control Symbol DD-DA&M(A)1379. ...

32 CFR 310.7 - Information requirements.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 2 2012-07-01 2012-07-01 false Information requirements. 310.7 Section 310.7 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) PRIVACY... § 310.6(d)(7) are assigned Report Control Symbol DD-DA&M(A)1379. ...

32 CFR 310.7 - Information requirements.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 2 2013-07-01 2013-07-01 false Information requirements. 310.7 Section 310.7 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) PRIVACY... § 310.6(d)(7) are assigned Report Control Symbol DD-DA&M(A)1379. ...

48 CFR 53.204-1 - Safeguarding classified information within industry (DD Form 254, DD Form 441).

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 2 2013-10-01 2013-10-01 false Safeguarding classified information within industry (DD Form 254, DD Form 441). 53.204-1 Section 53.204-1 Federal Acquisition....204-1 Safeguarding classified information within industry (DD Form 254, DD Form 441). The following...

48 CFR 53.204-1 - Safeguarding classified information within industry (DD Form 254, DD Form 441).

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Safeguarding classified information within industry (DD Form 254, DD Form 441). 53.204-1 Section 53.204-1 Federal Acquisition....204-1 Safeguarding classified information within industry (DD Form 254, DD Form 441). The following...

48 CFR 53.204-1 - Safeguarding classified information within industry (DD Form 254, DD Form 441).

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 2 2012-10-01 2012-10-01 false Safeguarding classified information within industry (DD Form 254, DD Form 441). 53.204-1 Section 53.204-1 Federal Acquisition....204-1 Safeguarding classified information within industry (DD Form 254, DD Form 441). The following...

48 CFR 53.204-1 - Safeguarding classified information within industry (DD Form 254, DD Form 441).

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 2 2014-10-01 2014-10-01 false Safeguarding classified information within industry (DD Form 254, DD Form 441). 53.204-1 Section 53.204-1 Federal Acquisition....204-1 Safeguarding classified information within industry (DD Form 254, DD Form 441). The following...

48 CFR 53.204-1 - Safeguarding classified information within industry (DD Form 254, DD Form 441).

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 2 2011-10-01 2011-10-01 false Safeguarding classified information within industry (DD Form 254, DD Form 441). 53.204-1 Section 53.204-1 Federal Acquisition....204-1 Safeguarding classified information within industry (DD Form 254, DD Form 441). The following...

Command and Control in Littoral Operations

DTIC Science & Technology

2016-05-13

mobility. Facing the broad spectrum of threats from mines, patrol boats, submarines and coastal defense cruise missiles, the ATF’s sea control was only...currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. 1. REPORT DATE (DD-MM-YYYY) 13-05-2016 2. REPORT TYPE...FINAL 3. DATES COVERED (From - To) 4. TITLE AND SUBTITLE Command and Control in Littoral Operations 5a. CONTRACT

77 FR 58109 - Submission for OMB Review; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-19

... Regulation Supplement (DFARS) part 245, Government Property, and the following related clauses and forms: DD Form 1149, Requisition and Invoice/Shipping Document; DD Form 1348-1A, DoD Single Line item Release/Receipt Document; DD Form 1639, Scrap Warranty; DD Form 1640, Request for Plant Clearance; DD Form 1641...

32 CFR 285.5 - Information requirements.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 2 2010-07-01 2010-07-01 false Information requirements. 285.5 Section 285.5 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) FREEDOM... requirements. Reporting requirements are in DoD 32 CFR part 286 and have been assigned Report Control Symbol DD...

32 CFR 216.6 - Information requirements.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 2 2014-07-01 2014-07-01 false Information requirements. 216.6 Section 216.6 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED...) have been assigned Report Control Symbol DD-P&R-(AR)-2038 in accordance with DoD 8910.1-M 2 . 2 Copies...

32 CFR 295.8 - Annual report.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 2 2012-07-01 2012-07-01 false Annual report. 295.8 Section 295.8 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) FREEDOM OF... report. The FOIA Annual Report, assigned Report Control System DD-PA (A) 1365, will be prepared by the...

32 CFR 295.8 - Annual report.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 2 2010-07-01 2010-07-01 false Annual report. 295.8 Section 295.8 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) FREEDOM OF... report. The FOIA Annual Report, assigned Report Control System DD-PA (A) 1365, will be prepared by the...

32 CFR 285.5 - Information requirements.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 2 2014-07-01 2014-07-01 false Information requirements. 285.5 Section 285.5 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) FREEDOM... requirements. Reporting requirements are in DoD 32 CFR part 286 and have been assigned Report Control Symbol DD...

32 CFR 295.8 - Annual report.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 2 2013-07-01 2013-07-01 false Annual report. 295.8 Section 295.8 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) FREEDOM OF... report. The FOIA Annual Report, assigned Report Control System DD-PA (A) 1365, will be prepared by the...

32 CFR 295.8 - Annual report.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 2 2011-07-01 2011-07-01 false Annual report. 295.8 Section 295.8 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) FREEDOM OF... report. The FOIA Annual Report, assigned Report Control System DD-PA (A) 1365, will be prepared by the...

32 CFR 295.8 - Annual report.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 2 2014-07-01 2014-07-01 false Annual report. 295.8 Section 295.8 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) FREEDOM OF... report. The FOIA Annual Report, assigned Report Control System DD-PA (A) 1365, will be prepared by the...

77 FR 6094 - Submission for OMB Review; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-07

... Acquisition Regulation Supplement (DFARS) part 245, Government Property; DD Form 1149, Requisition and Invoice/Shipping Document; DD Form 1348-1A, DoD Single Line item Release/Receipt Document; DD Form 1637, Notice of Acceptance of Inventory Schedules; DD Form 1639, Scrap Warranty; DD Form 1640, Request for Plant Clearance...

76 FR 63677 - Excepted Service

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-13

... Environmental Quality. (Energy/Climate Change). DEPARTMENT OF DEFENSE Office of the General Attorney-Advisor DD110104 7/29/2011 Counsel. (General). Washington Staff Assistant....... DD110112 7/20/2011 Headquarters.../2011 Executive Office for Counsel DJ110098 7/8/2011 United States Attorneys. Civil Rights Division...

U.S. National Arctic Strategy: Preparing Defensive Lines of Effort for the Arctic

DTIC Science & Technology

2014-04-01

publications hint at new political posturing and suggest China should develop a more assertive approach to the international debates on controlling ...currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. 1. REPORT DATE (DD-MM-YYYY) 01-04-2014 2. REPORT TYPE...importance of the Arctic, but lacks the infrastructure, command and control structure, and Arctic-capable assets to meet national strategic objectives

32 CFR 50.7 - Information requirements.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 1 2012-07-01 2012-07-01 false Information requirements. 50.7 Section 50.7 National Defense Department of Defense OFFICE OF THE SECRETARY OF DEFENSE PERSONNEL, MILITARY AND CIVILIAN... been assigned Report Control Symbol (RCS) DD-P&R(Q)2182 in accordance with DoD 8910.1-M. 12 12 See...

77 FR 40863 - Privacy Act of 1974; System of Records

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-11

... DEPARTMENT OF DEFENSE Office of the Secretary [Docket ID: DOD-2012-OS-0085] Privacy Act of 1974... Register Liaison Officer, Department of Defense. QNRO-31 System name: Software Security Risk Evaluations... the Department of Defense; DoDD 5240.2, DoD Counterintelligence (CI); DoDI 5240.8, Security...

Estimating DoD Transportation Spending: Analyses of Contract and Payment Transactions

DTIC Science & Technology

2007-01-01

the Defense Logistics Agency (DLA) and of expenditures by shipment material and volume (or cube). The analysis of DLA expenditures appears in Appendix...Defense Logistics Agency (DLA) not completely captured in DD350 data. Limited implementation outside the United States confines inferences that can be...Defense Information Systems Agency DLA Defense Logistics Agency DoD U.S. Department of Defense DoDAAC U.S. Department of Defense Activity Address Code

48 CFR 253.215-70 - DD Form 1547, Record of Weighted Guidelines Application.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false DD Form 1547, Record of... Forms 253.215-70 DD Form 1547, Record of Weighted Guidelines Application. Follow the procedures at PGI 253.215-70 for completing DD Form 1547. [71 FR 69495, Dec. 1, 2006] ...

32 CFR 728.13 - Application for care.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Duty § 728.13 Application for care. Possession of an ID card (a green colored DD 2 (with letter suffix... 32 National Defense 5 2010-07-01 2010-07-01 false Application for care. 728.13 Section 728.13 National Defense Department of Defense (Continued) DEPARTMENT OF THE NAVY PERSONNEL MEDICAL AND DENTAL CARE...

48 CFR 234.003 - Responsibilities.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Responsibilities. 234.003 Section 234.003 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE SPECIAL CATEGORIES OF CONTRACTING MAJOR SYSTEM ACQUISITION 234.003 Responsibilities. DoDD...

48 CFR 253.213-70 - Completion of DD Form 1155, Order for Supplies or Services.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Completion of DD Form 1155... Forms 253.213-70 Completion of DD Form 1155, Order for Supplies or Services. Follow the procedures at PGI 253.213-70 for completion of DD Form 1155. [71 FR 3413, Jan. 23, 2006] ...

48 CFR 247.372 - DD Form 1654, Evaluation of Transportation Cost Factors.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false DD Form 1654, Evaluation... Transportation in Supply Contracts 247.372 DD Form 1654, Evaluation of Transportation Cost Factors. Contracting personnel may use the DD Form 1654 to furnish information to the transportation office for development of...

76 FR 9837 - Excepted Service

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-22

... Associate GS110010......... 12/29/2010 Communications and Administrator for Marketing. Communications and.... Office of Deputy Director, DA110016......... 12/01/2010 Communications. Operations. Office of the General... DEFENSE Office of the Under Senior DD110019......... 12/03/2010 Secretary of Defense Communications...

48 CFR 215.404-70 - DD Form 1547, Record of Weighted Guidelines Method Application.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false DD Form 1547, Record of... TYPES CONTRACTING BY NEGOTIATION Contract Pricing 215.404-70 DD Form 1547, Record of Weighted Guidelines Method Application. Follow the procedures at PGI 215.404-70 for use of DD Form 1547 whenever a structured...

48 CFR 1846.673 - Distribution of DD Forms 250 and 250c.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Distribution of DD Forms 250 and 250c. 1846.673 Section 1846.673 Federal Acquisition Regulations System NATIONAL AERONAUTICS... Reports 1846.673 Distribution of DD Forms 250 and 250c. (a) DD Forms 250 and 250c shall be distributed in...

32 CFR 751.10 - Form of claim.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Claims Against the United States § 751.10 Form of claim. The claim should be submitted on DD Form 1842 (Claim for Personal Property) accompanied by DD Form 1844 (List of Property). If DD Forms 1842 and 1844 1...

32 CFR 881.5 - If an application is approved.

Code of Federal Regulations, 2010 CFR

2010-07-01

... be honorable, HQ AFPC/DPPRSO issues the applicant a DD Form 256AF, Honorable Discharge, and a DD Form... (formerly AFR 35-6). (b) Enter a military grade on the DD Form 214 only if the Administrator of Veterans' Affairs requests it. (c) Enter a pay grade on the DD Form 214 only for individuals who were killed or...

Navy DD(X), CG(X), and LCS Ship Acquisition Programs: Oversight Issues and Options for Congress

DTIC Science & Technology

2006-02-28

NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e . TASK NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) Congressional...Systems ( NGSS ) division. The Navy has not procured any frigates since FY1984, when the last FFG-7 was procured. 20 In earlier years, some Navy surface...Defense for Acquisition, Technology, and Logistics (USD AT&L) in February 2004, the first DD(X) would be built by NGSS , the second DD(X) would be built GD

78 FR 16713 - Excepted Service

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-18

... SB130002 1/16/2013 Communications and Secretary for the Public Liaison. Office of Communications and Public....... DA130022 1/25/2013 Assistant Secretary for Congressional Relations. DEPARTMENT OF COMMERCE Office of... OF DEFENSE Office of Assistant Speechwriter (2)..... DD130025 1/11/2013 Secretary of Defense (Public...

Forecasting Workload for Defense Logistics Agency Distribution

DTIC Science & Technology

2014-12-01

Distribution workload ...........................18 Monthly DD Sales for the four primary supply chains ( Avn , Land, Maritime, Ind HW) plotted to...average AVN Aviation BSM Business Systems Modernization CIT consumable items transfer C&E Construction and Equipment C&T Clothing...992081.437 See Figure 2 below for the graphical output of the linear regression. Monthly DD Sales for the four primary supply chains ( Avn , Land

32 CFR 1630.13 - Class 1-D-D: Deferment for certain members of a reserve component or student taking military...

Code of Federal Regulations, 2010 CFR

2010-07-01

... reserve component or student taking military training. 1630.13 Section 1630.13 National Defense Other...: Deferment for certain members of a reserve component or student taking military training. In Class 1-D-D... been commissioned therein upon graduation from an Officer's Candidate School of such Armed Force and...

Self-Reported Pleasantness Ratings and Examiner-Coded Defensiveness in Response to Touch in Children with ASD: Effects of Stimulus Material and Bodily Location

ERIC Educational Resources Information Center

Cascio, Carissa J.; Lorenzi, Jill; Baranek, Grace T.

2016-01-01

Tactile defensiveness, characterized by behavioral hyperresponsiveness and negative emotional responses to touch, is a common manifestation of aberrant sensory processing in autism spectrum disorders (ASD) and other developmental disabilities (DD). Variations in tactile defensiveness with the properties of the stimulus and the bodily site of…

75 FR 60434 - Proposed Collection; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-30

... Certificate of Insurance, DD Form 2400; Civil Aircraft Landing Permit, DD Form 2401; Civil Aircraft Hold Harmless Agreement, DD Form 2402; OMB Control Number 0701-0050. Needs and Uses: The collection of...

77 FR 6089 - Proposed Collection; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-07

...) 767-6870. Title; Associated Form; and OMB Number: Certification of Qualified Products; DD Form 1718... (QMLs). DD Form 1718, ``Certification of Qualified Products'' records and certifies, from the... of the DD Form 1718 is essential in maintaining the integrity of the qualification program. Dated...

32 CFR 1285.7 - Reports.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 6 2010-07-01 2010-07-01 false Reports. 1285.7 Section 1285.7 National Defense... AGENCY FREEDOM OF INFORMATION ACT PROGRAM § 1285.7 Reports. The reporting requirement outlined in this rule is assigned Report Control Symbol DD-PA(A)1365 and will be prepared according to part 286, subpart...

32 CFR 1285.7 - Reports.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 6 2012-07-01 2012-07-01 false Reports. 1285.7 Section 1285.7 National Defense... AGENCY FREEDOM OF INFORMATION ACT PROGRAM § 1285.7 Reports. The reporting requirement outlined in this rule is assigned Report Control Symbol DD-PA(A)1365 and will be prepared according to part 286, subpart...

Air and Missile Defense Radar (AMDR)

DTIC Science & Technology

2015-12-01

Selected Acquisition Report ( SAR ) RCS: DD-A&T(Q&A)823-384 Air and Missile Defense Radar (AMDR) As of FY 2017 President’s Budget Defense Acquisition...Management Information Retrieval (DAMIR) March 8, 2016 11:04:34 UNCLASSIFIED AMDR December 2015 SAR March 8, 2016 11:04:34 UNCLASSIFIED 2 Table...OSD - Office of the Secretary of Defense O&S - Operating and Support PAUC - Program Acquisition Unit Cost AMDR December 2015 SAR March 8, 2016 11:04:34

78 FR 69391 - Submission for OMB Review; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-19

...: Title, Associated Form and OMB Number: DOD Loan Repayment Program (LRP); DD Form 2475; OMB Number 0704... the program forward the DD Form 2475 ``DOD Loan Repayment Program (LRP) Annual Application,'' to their... Military Service Personnel Office forwards the DD Form 2475 to the lending institution. For active-duty...

77 FR 72334 - Submission for OMB Review; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-05

.... Title, Form, and OMB Number: Militarily Critical Technical Data Agreement, DD Form 2354; OMB Control... unclassified DoD-controlled militarily critical technical data must certify on DD Form 2345, Militarily... requirements and is so indicated on the Privacy Act Statement of DD Form 2345. Affected Public: Individuals or...

78 FR 28579 - Proposed Collection; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-15

...; Associated Form; and OMB Control Number: DOD Loan Repayment Program (LRP); DD Form 2475, OMB Number 0704-0152... the program forward the DD Form 2475, ``DOD Loan Repayment Program (LRP) Annual Application,'' to... Components, the Military Service Personnel Office forwards the DD Form 2475 to the lending institution. For...

Defense related decadienal elicits membrane lipid remodeling in the diatom Phaeodactylum tricornutum

PubMed Central

Sabharwal, Tanya; Sathasivan, Kanagasabapathi

2017-01-01

Diatoms rapidly release extracellular oxylipins (oxygenated lipids) including polyunsaturated aldehydes in response to herbivory and other stresses. Oxylipins have several defense-related activities including inhibition of reproduction in herbivores and signaling to distant diatoms. Physiological changes in diatoms exposed to varying levels of oxylipins are only beginning to be understood. In this study, Phaeodactylum tricornutum cultures were treated with sublethal concentrations of the polyunsaturated aldehyde trans,trans-2,4-decadienal (DD) to assess effects on lipid composition and membrane permeability. In cells treated with DD for 3 hr, all measured saturated and unsaturated fatty acids significantly decreased (0.46–0.69 fold of levels in solvent control cells) except for 18:2 (decreased but not significantly). The decrease was greater in the polyunsaturated fatty acid pool than the saturated and monounsaturated fatty acid pool. Analysis of lipid classes revealed increased abundances of phosphatidylethanolamine and phosphatidylcholine at 3 and 6 hr. Concomitantly, these and other membrane lipids exhibited increased saturated and monounsaturated acyl chains content relative to polyunsaturated acyl chains compared to control cells. Evidence of decreased plasma membrane permeability in DD treated cells was obtained, based on reduced uptake of two of three dyes relative to control cells. Additionally, cells pre-conditioned with a sublethal DD dose for 3 hr then treated with a lethal DD dose for 2 hr exhibited greater membrane integrity than solvent pre-conditioned control cells that were similarly treated. Taken together, the data are supportive of the hypothesis that membrane remodeling induced by sublethal DD is a key element in the development of cellular resistance in diatoms to varying and potentially toxic levels of polyunsaturated aldehydes in environments impacted by herbivory or other stresses. PMID:28582415

Integrated Defensive Electronic Countermeasures (IDECM)

DTIC Science & Technology

2015-12-01

Selected Acquisition Report ( SAR ) RCS: DD-A&T(Q&A)823-418 Integrated Defensive Electronic Countermeasures (IDECM) As of FY 2017 President’s Budget...Defense Acquisition Management Information Retrieval (DAMIR) March 10, 2016 08:58:42 UNCLASSIFIED IDECM December 2015 SAR March 10, 2016 08:58:42... SAR March 10, 2016 08:58:42 UNCLASSIFIED 3 PB - President’s Budget PE - Program Element PEO - Program Executive Officer PM - Program Manager POE

Growth curves and morphology of three Treponema subtypes isolated from digital dermatitis in cattle.

PubMed

Döpfer, D; Anklam, K; Mikheil, D; Ladell, P

2012-09-01

Digital dermatitis (DD) is an infectious claw disease of cattle that causes painful lesions, principally along the coronary band of the claws. In the US alone, the estimated economic impact of DD is estimated to be $190 million. The etiology of DD remains unclear and there is no reliable laboratory test, so DD is most often diagnosed clinically. Spirochetal bacteria of the genera Treponema have been implicated in DD infections following their isolation using culture techniques, serological detection of bovine antibodies against treponemes, and amplification of treponemal 16s DNA sequences by PCR. During in vitro growth of spirochetes and treponemes isolated from DD, morphological changes have been observed indicating the presence of a spiral form and an encysted form. It is not known why encysted forms appear or what role they have in the progression of DD. The current study established growth curves for three subtypes of treponemes, Treponema denticola-like, Treponema phagedenis-like, and Treponema medium-like, while photographically monitoring changes in morphology. In addition to observing spiral and encysted forms, two intermediate forms were also observed. These appeared as either spiral forms with spherical bodies or as enveloped clusters of granules. The observation of encysted forms adds further support to the theory that treponemes causing recurrent infections deep in bovine skin have mechanisms to facilitate persistence and the chronic character of DD. Published by Elsevier Ltd.

Training Detractors in FORSCOM Divisions and How They Are Handled

DTIC Science & Technology

1980-05-01

mandatory training on conducting effective combat training, and explored four resource areas No. DD JAN 1473 EDITION OF I NOV 65 IS OBSOLETE...INSTITUTIONAL RSCH I USA AIR DEFENSE SCHOOL AITN: AISA -CD-MS 1 USAA|)S-LIBRARY-DOCUMENIS I USA AIR DEFENSE BOAR) ATIN: FILES REPOSITORY I USA sERGEANTS

Integrating the Full Range of Security Cooperation Programs into Air Force Planning: An Analytic Primer

DTIC Science & Technology

2011-01-01

Exchange Program ( IEP ) Authority 10 U.S.C. §2358, “Research and development projects” Processes and agreements IEP agreements with the...2015.4, “Defense Research, Development, Test and Evaluation (RDT&E) Information Exchange Program ( IEP )”; DoDD 5134.1, “Under Secretary of Defense for

78 FR 68824 - Proposed Collection; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-15

... Number: Automated Repatriation Reporting System; DD Form 2585; OMB Control Number 0704-0334. Needs and... States. The information obtained from the DD Form 2585 is entered into an automated system; a series of... the protection, evacuation and repatriation of U.S. citizens in threatened areas overseas. The DD Form...

75 FR 38087 - Proposed Collection; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-01

... Repatriation Reporting System, DD Form 2585, OMB Control Number 0704- 0334. Needs and Uses: This information... from the DD Form 2585 is entered into an automated system; a series of reports is accessible to DoD... DD Form 2585, ``Repatriation Processing Center Processing Sheet'', has numerous functions, but is...

48 CFR 247.370 - DD Form 1384, Transportation Control and Movement Document.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false DD Form 1384, Transportation Control and Movement Document. 247.370 Section 247.370 Federal Acquisition Regulations System... Transportation in Supply Contracts 247.370 DD Form 1384, Transportation Control and Movement Document. The...

Recurring Reports of Civilian Employment and Payrolls

DTIC Science & Technology

1989-09-11

Department of Defense -, ,• INSTRUCTION September 11, 1989AD-A272 768 NME 701 DA& M SUBJECT: Recurring Reports of Civilian Employment and Payrolls...Positions and Modification of TAPER Authority to Permit Promotion and Reassignment of these Employees," July 30, 1979 ( m ) Federal Personnel Manual (FPM...L. DD32 On-Site Inspection Agency (OSIA) M . DDOI: 1. Office of the Secretary of Defense (OSD) 2. Joint Chiefs of Staff (JCS)* 3. Defense Security

41 CFR 101-26.4904-416 - DD Form 416: Purchase Request for Coal, Coke, or Briquettes.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 41 Public Contracts and Property Management 2 2011-07-01 2007-07-01 true DD Form 416: Purchase Request for Coal, Coke, or Briquettes. 101-26.4904-416 Section 101-26.4904-416 Public Contracts and... DD Form 416: Purchase Request for Coal, Coke, or Briquettes. Note: The form illustrated in § 101-26...

41 CFR 101-26.4904-416 - DD Form 416: Purchase Request for Coal, Coke, or Briquettes.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 41 Public Contracts and Property Management 2 2014-07-01 2012-07-01 true DD Form 416: Purchase Request for Coal, Coke, or Briquettes. 101-26.4904-416 Section 101-26.4904-416 Public Contracts and... DD Form 416: Purchase Request for Coal, Coke, or Briquettes. Note: The form illustrated in § 101-26...

41 CFR 101-26.4904-416 - DD Form 416: Purchase Request for Coal, Coke, or Briquettes.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 41 Public Contracts and Property Management 2 2010-07-01 2010-07-01 true DD Form 416: Purchase Request for Coal, Coke, or Briquettes. 101-26.4904-416 Section 101-26.4904-416 Public Contracts and... DD Form 416: Purchase Request for Coal, Coke, or Briquettes. Note: The form illustrated in § 101-26...

41 CFR 101-26.4904-416 - DD Form 416: Purchase Request for Coal, Coke, or Briquettes.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 41 Public Contracts and Property Management 2 2012-07-01 2012-07-01 false DD Form 416: Purchase Request for Coal, Coke, or Briquettes. 101-26.4904-416 Section 101-26.4904-416 Public Contracts and... DD Form 416: Purchase Request for Coal, Coke, or Briquettes. Note: The form illustrated in § 101-26...

41 CFR 101-26.4904-416 - DD Form 416: Purchase Request for Coal, Coke, or Briquettes.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 41 Public Contracts and Property Management 2 2013-07-01 2012-07-01 true DD Form 416: Purchase Request for Coal, Coke, or Briquettes. 101-26.4904-416 Section 101-26.4904-416 Public Contracts and... DD Form 416: Purchase Request for Coal, Coke, or Briquettes. Note: The form illustrated in § 101-26...

Medical Services: Department of Defense Veterinary/Medical Laboratory Food Safety and Quality Assurance Program

DTIC Science & Technology

1995-02-01

ice (cream) milk and yogurt processing at the retail/user level (chap 5). o Revises and prescribes the use of DD Form 2385 (Microbiological Quality... yogurt , sandwiches and spreads, salad type convenience foods, and other processed/ p r e – p a c k a g e d a n d r e a d y – t o – e a t ( R T E ) f o o...dairy products to include, but not be limited to, fresh and cultured dairy products, frozen desserts, soft serve ice cream/milk and yogurt mix. (3) RTE

32 CFR 45.2 - Applicability and scope.

Code of Federal Regulations, 2010 CFR

2010-07-01

... CERTIFICATE OF RELEASE OR DISCHARGE FROM ACTIVE DUTY (DD FORM 214/5 SERIES) § 45.2 Applicability and scope. (a... on the preparation and distribution of DD Forms 214, 214WS, 215 (Appendices A, B, and C) which record... Management and Personnel) approval is obtained.) DD Forms 214 and 215 (or their substitutes) will provide: (1...

48 CFR 1852.246-72 - Material inspection and receiving report.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Receiving Report (DD Form 250 series) prepared in __ [Insert number of copies, including original] copies, an original and __ copies [Insert number of copies]. (b) The Contractor shall prepare the DD Form 250 in accordance with NASA FAR Supplement 1846.6. The Contractor shall enclose the copies of the DD Form...

48 CFR 245.606-70 - Instructions for completing DD Form 1342, DoD Property Record.

Code of Federal Regulations, 2010 CFR

2010-10-01

... PROPERTY Reporting, Redistribution, and Disposal of Contractor Inventory 245.606-70 Instructions for completing DD Form 1342, DoD Property Record. (a) The contractor shall list excess industrial plant equipment... completing DD Form 1342, DoD Property Record. 245.606-70 Section 245.606-70 Federal Acquisition Regulations...

76 FR 82003 - Submission for Review: DD 1918 Establishment Information Form, DD 1919 Wage Data Collection Form...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-29

... 1919 Wage Data Collection Form, DD 1919C Wage Data Collection Continuation Form AGENCY: U.S. Office of Personnel Management. ACTION: 30-Day Notice and request for comments. SUMMARY: The U.S. Office of Personnel... the Paperwork Reduction Act of 1995 (Pub. L. 104-13, 44 U.S.C. chapter 35) as amended by the Clinger...

Joint Chiefs of Staff > About > Joint Staff Inspector General

Science.gov Websites

DD Form 2949, Joint Inspector General Action Request (available at the link below). However, if you action if you intentionally make false statements. When you sign a DD Form 2949, you are signing a . DD Form 2949, Joint Inspector General Action Request Click here to expand content Click here to

Information Management for an Automated Battlefield Command and Control System

DTIC Science & Technology

1980-05-01

DD) I u FJ i EDITION OF" I NOV 65, IS OSSOLIETIES"-- iUnclassified1SECURITY CLASSIFICATION OF THIS PAGE (When Data Entered) t// II. Unclassified...DIRELIOk oý INSTIhJTIONAL RSCH I USA AIP DEFENSE SCHOUL ATTN: AISA -CU)-MS I iJSAADS-LIBRARY-OUCUMLNTS) I USA AIW DEFENSE ZL~N HDAU IN: fILLS

32 CFR 887.7 - Persons separated under other than honorable conditions (undesirable or bad conduct) or...

Code of Federal Regulations, 2010 CFR

2010-07-01

.... However, an official photocopy of the report of separation or certificate of discharge (DD Form 214... written request of the member. (a) On the DD Forms 214 issued before October 1, 1979, the following items...). (b) For DD Forms 214 issued after October 1, 1979, send one copy with the Special Additional...

The Role of Western Germany in West European Defense

DTIC Science & Technology

1966-04-08

Ralph. Modern German History. New York: E. P. Dutton & Co., Inc., 1964. (DD175 F5) 34. German Research Association. Germany: Franz Steiner Verlag Gmb...and Rudolf , Walter. This Germany. New York: New York Graphic Society Publishers, Ltd., 1954. (DD257 L42) 39. Heidenheimer, Arnold J. The Government...202-07, 243. 47. Lauder, K. H. A Brief Review of Science and Technoloc in Western Germany. London: HIISO, 1955. (Q18 G4G7) 48. Leonhardt, Rudolf Walter

75 FR 23251 - Submission for OMB Review; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-03

... and OMB Number: DOD Loan Repayment Program (LRP); DD Form 2475, OMB Number 0704-0152. Type of Request... Reserve service for a specific obligated period. Applicants who qualify for the program forward the DD... Personnel Office forwards the DD Form 2475 to the lending institution. For active-duty Service, the Service...

75 FR 52313 - Submission for OMB Review; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-25

.... Title, Form and OMB Number: Request for Armed Forces Participation in Public Events (Non-Aviation), DD Form 2536 and Request for Military Aerial Support, DD Form 2535; OMB Number 0704-0290. Type of Request...

Structural Study of the RIPoptosome Core Reveals a Helical Assembly for Kinase Recruitment

PubMed Central

2015-01-01

Receptor interaction protein kinase 1 (RIP1) is a molecular cell-fate switch. RIP1, together with Fas-associated protein with death domain (FADD) and caspase-8, forms the RIPoptosome that activates apoptosis. RIP1 also associates with RIP3 to form the necrosome that triggers necroptosis. The RIPoptosome assembles through interactions between the death domains (DDs) of RIP1 and FADD and between death effector domains (DEDs) of FADD and caspase-8. In this study, we analyzed the overall structure of the RIP1 DD/FADD DD complex, the core of the RIPoptosome, by negative-stain electron microscopy and modeling. The results show that RIP1 DD and FADD DD form a stable complex in vitro similar to the previously described Fas DD/FADD DD complex, suggesting that the RIPoptosome and the Fas death-inducing signaling complex share a common assembly mechanism. Both complexes adopt a helical conformation that requires type I, II, and III interactions between the death domains. PMID:25119434

Cooperative Engagement Capability (CEC)

DTIC Science & Technology

2015-12-01

Selected Acquisition Report ( SAR ) RCS: DD-A&T(Q&A)823-582 Cooperative Engagement Capability (CEC) As of FY 2017 President’s Budget Defense...Acquisition Management Information Retrieval (DAMIR) March 17, 2016 12:13:59 UNCLASSIFIED CEC December 2015 SAR March 17, 2016 12:13:59 UNCLASSIFIED 2...Document OSD - Office of the Secretary of Defense O&S - Operating and Support PAUC - Program Acquisition Unit Cost CEC December 2015 SAR March 17, 2016 12

Who Should Have the Hammer? Leadership of SSTR Planning and Operations

DTIC Science & Technology

2009-10-23

Security Presidential Directive/NSPD-44 was produced which clarified governmental management of reconstruction and stabilization efforts. 1 Additionally...President, “ Management of Interagency Efforts Concerning Reconstruction and Stabilization, pp. 1 2 U.S. Department of Defense, DoDD 3000.05, pp.1 3 U.S...from regional and local State personnel, and conditional stand up of teams within the Interagency Management System. 16 Defense Department guidance

A putative low-molecular-mass penicillin-binding protein (PBP) of Mycobacterium smegmatis exhibits prominent physiological characteristics of DD-carboxypeptidase and beta-lactamase.

PubMed

Bansal, Ankita; Kar, Debasish; Murugan, Rajagopal A; Mallick, Sathi; Dutta, Mouparna; Pandey, Satya Deo; Chowdhury, Chiranjit; Ghosh, Anindya S

2015-05-01

DD-carboxypeptidases (DD-CPases) are low-molecular-mass (LMM) penicillin-binding proteins (PBPs) that are mainly involved in peptidoglycan remodelling, but little is known about the dd-CPases of mycobacteria. In this study, a putative DD-CPase of Mycobacterium smegmatis, MSMEG_2433 is characterized. The gene for the membrane-bound form of MSMEG_2433 was cloned and expressed in Escherichia coli in its active form, as revealed by its ability to bind to the Bocillin-FL (fluorescent penicillin). Interestingly, in vivo expression of MSMEG_2433 could restore the cell shape oddities of the septuple PBP mutant of E. coli, which was a prominent physiological characteristic of DD-CPases. Moreover, expression of MSMEG_2433 in trans elevated beta-lactam resistance in PBP deletion mutants (ΔdacAdacC) of E. coli, strengthening its physiology as a dd-CPase. To confirm the biochemical reason behind such physiological behaviours, a soluble form of MSMEG_2433 (sMSMEG_2433) was created, expressed and purified. In agreement with the observed physiological phenomena, sMSMEG_2433 exhibited DD-CPase activity against artificial and peptidoglycan-mimetic DD-CPase substrates. To our surprise, enzymic analyses of MSMEG_2433 revealed efficient deacylation for beta-lactam substrates at physiological pH, which is a unique characteristic of beta-lactamases. In addition to the MSMEG_2433 active site that favours dd-CPase activity, in silico analyses also predicted the presence of an omega-loop-like region in MSMEG_2433, which is an important determinant of its beta-lactamase activity. Based on the in vitro, in vivo and in silico studies, we conclude that MSMEG_2433 is a dual enzyme, possessing both DD-CPase and beta-lactamase activities. © 2015 The Authors.

48 CFR 245.7101-3 - DD Form 1348-1, DoD Single Line Item Release/Receipt Document.

Code of Federal Regulations, 2010 CFR

2010-10-01

... PROPERTY Plant Clearance Forms 245.7101-3 DD Form 1348-1, DoD Single Line Item Release/Receipt Document. Use for shipments of excess industrial plant equipment and contractor inventory redistribution system...

Combat Rescue Helicopter (CRH)

DTIC Science & Technology

2015-12-01

Selected Acquisition Report ( SAR ) RCS: DD-A&T(Q&A)823-479 Combat Rescue Helicopter (CRH) As of FY 2017 President’s Budget Defense Acquisition...Management Information Retrieval (DAMIR) March 17, 2016 15:16:47 UNCLASSIFIED CRH December 2015 SAR March 17, 2016 15:16:47 UNCLASSIFIED 2 Table of...Office of the Secretary of Defense O&S - Operating and Support PAUC - Program Acquisition Unit Cost CRH December 2015 SAR March 17, 2016 15:16:47

DDG-1000 Zumwalt Class Destroyer (DDG-1000)

DTIC Science & Technology

2015-12-01

Selected Acquisition Report ( SAR ) RCS: DD-A&T(Q&A)823-197 DDG 1000 Zumwalt Class Destroyer (DDG 1000) As of FY 2017 President’s Budget Defense...Acquisition Management Information Retrieval (DAMIR) March 23, 2016 15:17:06 UNCLASSIFIED DDG 1000 December 2015 SAR March 23, 2016 15:17:06...Requirements Document OSD - Office of the Secretary of Defense O&S - Operating and Support PAUC - Program Acquisition Unit Cost DDG 1000 December 2015 SAR

DDG 51 Arleigh Burke Class Guided Missile Destroyer (DDG 51)

DTIC Science & Technology

2015-12-01

Selected Acquisition Report ( SAR ) RCS: DD-A&T(Q&A)823-180 DDG 51 Arleigh Burke Class Guided Missile Destroyer (DDG 51) As of FY 2017 President’s...Budget Defense Acquisition Management Information Retrieval (DAMIR) UNCLASSIFIED DDG 51 December 2015 SAR UNCLASSIFIED 2 Table of Contents Common Acronyms...Defense O&S - Operating and Support PAUC - Program Acquisition Unit Cost DDG 51 December 2015 SAR UNCLASSIFIED 3 PB - President’s Budget PE - Program

32 CFR 45.1 - Purpose.

Code of Federal Regulations, 2010 CFR

2010-07-01

... RELEASE OR DISCHARGE FROM ACTIVE DUTY (DD FORM 214/5 SERIES) § 45.1 Purpose. (a) This document revises 32 CFR part 45. (b) Prescribes procedures concerning the preparation and distribution of revised DD Form...

77 FR 71174 - Proposed Collection; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-29

...-0039. Title; Associated Form; and OMB Number: Continued Health Care Benefit Program, DD Form 2837; OMB... order to be eligible for health care coverage under CHCBP, an individual must first enroll in CHCBP. DD...

75 FR 60421 - Submission for OMB Review; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-30

.... Title and OMB Number: Application for Homeowners Assistance; DD Form 1607; OMB Control Number 0704-0463... met. Eligible homeowners use the DD Form 1607, ``Application for Homeowners Assistance'' to apply. The...

32 CFR 169a.8 - Inventory and review schedule (Report Control Symbol DD-P&L(A)).

Code of Federal Regulations, 2014 CFR

2014-07-01

... (DIA) Shall be submitted to the Assistant Secretary of Defense Production and Logistics) (ASD(P&L... to this part provides the codes and explanations for functional areas and Appendix B to this part...

32 CFR 169a.8 - Inventory and review schedule (Report Control Symbol DD-P&L(A)).

Code of Federal Regulations, 2012 CFR

2012-07-01

... (DIA) Shall be submitted to the Assistant Secretary of Defense Production and Logistics) (ASD(P&L... to this part provides the codes and explanations for functional areas and Appendix B to this part...

77 FR 49029 - Excepted Service

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-15

... the Special DD120075 6/1/2012 Assistant Assistant(Cyber Secretary of Policy). Defense, Global... for Legislation. Department of Homeland Security. Office of the Chief Special Assistant.. DM120131 6/1... Security, Issues. Democracy and Human Rights. Department of Transportation.... Secretary Special Assistant...

76 FR 78316 - Excepted Service

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-16

... Assistant Confidential Assistant DA120006 10/25/2011 Secretary for Congressional Relations. Natural... Assistant Secretary of Speechwriter DD110134 10/21/2011 Defense (Public Affairs). Office of the Secretary of.../2011 Congressional Affairs. Office of Communications and Confidential Assistant DB120008 10/20/2011...

48 CFR 1253.227-70 - Conveyance of invention rights acquired by the Government.

Code of Federal Regulations, 2010 CFR

2010-10-01

... report inventions made in the course of contract performance, as specified in 1227.305-4: DD Form 882, Report of Inventions and Subcontracts. DD Form 882 can be found at http://www.aro.army.mil/forms/d882.pdf. ...

32 CFR 881.8 - Disposition of documents.

Code of Federal Regulations, 2010 CFR

2010-07-01

... documents. (a) File a copy of the application, supporting evidence, and DD Form 214 in the Master Personnel... cases. Send copies of DD Form 214 to: (1) The applicant. (2) The Veterans' Administration. (3) HQ AFPC...

75 FR 81244 - Submission for OMB Review; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-27

... Supplement (DFARS) Part 204, Administrative Matters, and related clauses at DFARS 252.204; DD Form 2051, Request for Assignment of a Commercial and Government Entity (CAGE) Code, and DD Form 2051-1, Request for...

Evidence that the Dictyostelium Dd-STATa protein is a repressor that regulates commitment to stalk cell differentiation and is also required for efficient chemotaxis.

PubMed

Mohanty, S; Jermyn, K A; Early, A; Kawata, T; Aubry, L; Ceccarelli, A; Schaap, P; Williams, J G; Firtel, R A

1999-08-01

Dd-STATa is a structural and functional homologue of the metazoan STAT (Signal Transducer and Activator of Transcription) proteins. We show that Dd-STATa null cells exhibit several distinct developmental phenotypes. The aggregation of Dd-STATa null cells is delayed and they chemotax slowly to a cyclic AMP source, suggesting a role for Dd-STATa in these early processes. In Dd-STATa null strains, slug-like structures are formed but they have an aberrant pattern of gene expression. In such slugs, ecmB/lacZ, a marker that is normally specific for cells on the stalk cell differentiation pathway, is expressed throughout the prestalk region. Stalk cell differentiation in Dictyostelium has been proposed to be under negative control, mediated by repressor elements present in the promoters of stalk cell-specific genes. Dd-STATa binds these repressor elements in vitro and the ectopic expression of ecmB/lacZ in the null strain provides in vivo evidence that Dd-STATa is the repressor protein that regulates commitment to stalk cell differentiation. Dd-STATa null cells display aberrant behavior in a monolayer assay wherein stalk cell differentiation is induced using the stalk cell morphogen DIF. The ecmB gene, a general marker for stalk cell differentiation, is greatly overinduced by DIF in Dd-STATa null cells. Also, Dd-STATa null cells are hypersensitive to DIF for expression of ST/lacZ, a marker for the earliest stages in the differentiation of one of the stalk cell sub-types. We suggest that both these manifestations of DIF hypersensitivity in the null strain result from the balance between activation and repression of the promoter elements being tipped in favor of activation when the repressor is absent. Paradoxically, although Dd-STATa null cells are hypersensitive to the inducing effects of DIF and readily form stalk cells in monolayer assay, the Dd-STATa null cells show little or no terminal stalk cell differentiation within the slug. Dd-STATa null slugs remain developmentally arrested for several days before forming very small spore masses supported by a column of apparently undifferentiated cells. Thus, complete stalk cell differentiation appears to require at least two events: a commitment step, whereby the repression exerted by Dd-STATa is lifted, and a second step that is blocked in a Dd-STATa null organism. This latter step may involve extracellular cAMP, a known repressor of stalk cell differentiation, because Dd-STATa null cells are abnormally sensitive to the inhibitory effects of extracellular cyclic AMP.

DTIC (Defense Technical Information Center) Model Action Plan for Incorporating DGIS (DOD Gateway Information System) Capabilities.

DTIC Science & Technology

1986-05-01

Information System (DGIS) is being developed to provide the DD crmjnj t with a modern tool to access diverse dtabaiees and extract information products...this community with a modern tool for accessing these databases and extracting information products from them. Since the Defense Technical Information...adjunct to DROLS xesults. The study , thereor. centerd around obtaining background information inside the unit on that unit’s users who request DROLS

SSN 774 Virginia Class Submarine (SSN 774)

DTIC Science & Technology

2015-12-01

Selected Acquisition Report ( SAR ) RCS: DD-A&T(Q&A)823-516 SSN 774 Virginia Class Submarine (SSN 774) As of FY 2017 President’s Budget Defense...Acquisition Management Information Retrieval (DAMIR) March 8, 2016 11:22:44 UNCLASSIFIED SSN 774 December 2015 SAR March 8, 2016 11:22:44 UNCLASSIFIED 2...Document OSD - Office of the Secretary of Defense O&S - Operating and Support PAUC - Program Acquisition Unit Cost SSN 774 December 2015 SAR March 8

Ground/Air Task Oriented Radar (G/ATOR)

DTIC Science & Technology

2015-12-01

Selected Acquisition Report ( SAR ) RCS: DD-A&T(Q&A)823-386 Ground/Air Task Oriented Radar (G/ATOR) As of FY 2017 President’s Budget Defense...Acquisition Management Information Retrieval (DAMIR) March 18, 2016 06:47:59 UNCLASSIFIED G/ATOR December 2015 SAR March 18, 2016 06:47:59 UNCLASSIFIED 2...Document OSD - Office of the Secretary of Defense O&S - Operating and Support PAUC - Program Acquisition Unit Cost G/ATOR December 2015 SAR March 18

Small Diameter Bomb Increment II (SDB II)

DTIC Science & Technology

2015-12-01

Selected Acquisition Report ( SAR ) RCS: DD-A&T(Q&A)823-439 Small Diameter Bomb Increment II (SDB II) As of FY 2017 President’s Budget Defense...Acquisition Management Information Retrieval (DAMIR) March 23, 2016 16:19:13 UNCLASSIFIED SDB II December 2015 SAR March 23, 2016 16:19:13 UNCLASSIFIED...Document OSD - Office of the Secretary of Defense O&S - Operating and Support PAUC - Program Acquisition Unit Cost SDB II December 2015 SAR March 23

Littoral Combat Ship Mission Modules (LCS MM)

DTIC Science & Technology

2015-12-01

Selected Acquisition Report ( SAR ) RCS: DD-A&T(Q&A)823-443 Littoral Combat Ship Mission Modules (LCS MM) As of FY 2017 President’s Budget Defense...Acquisition Management Information Retrieval (DAMIR) March 9, 2016 08:50:12 UNCLASSIFIED LCS MM December 2015 SAR March 9, 2016 08:50:12 UNCLASSIFIED...Document OSD - Office of the Secretary of Defense O&S - Operating and Support PAUC - Program Acquisition Unit Cost LCS MM December 2015 SAR March 9

F-35 Joint Strike Fighter Aircraft (F-35)

DTIC Science & Technology

2015-12-01

Selected Acquisition Report ( SAR ) RCS: DD-A&T(Q&A)823-198 F-35 Joint Strike Fighter Aircraft (F-35) As of FY 2017 President’s Budget Defense...Acquisition Management Information Retrieval (DAMIR) March 21, 2016 08:47:09 UNCLASSIFIED F-35 December 2015 SAR March 21, 2016 08:47:09 UNCLASSIFIED 2...Document OSD - Office of the Secretary of Defense O&S - Operating and Support PAUC - Program Acquisition Unit Cost F-35 December 2015 SAR March 21

LHA 6 America Class Amphibious Assault Ship (LHA 6)

DTIC Science & Technology

2015-12-01

Selected Acquisition Report ( SAR ) RCS: DD-A&T(Q&A)823-333 LHA 6 America Class Amphibious Assault Ship (LHA 6) As of FY 2017 President’s Budget...Defense Acquisition Management Information Retrieval (DAMIR) March 8, 2016 09:40:04 UNCLASSIFIED LHA 6 December 2015 SAR March 8, 2016 09:40:04...Requirements Document OSD - Office of the Secretary of Defense O&S - Operating and Support PAUC - Program Acquisition Unit Cost LHA 6 December 2015 SAR March

Joint Precision Approach and Landing System Increment 1A (JPALS Inc 1A)

DTIC Science & Technology

2015-12-01

Selected Acquisition Report (SAR) RCS: DD-A&T(Q&A)823-238 Joint Precision Approach and Landing System Increment 1A (JPALS Inc 1A) As of FY 2017...President’s Budget Defense Acquisition Management Information Retrieval (DAMIR) March 10, 2016 11:30:56 UNCLASSIFIED JPALS Inc 1A December 2015 SAR...Fiscal Year FYDP - Future Years Defense Program ICE - Independent Cost Estimate IOC - Initial Operational Capability Inc - Increment JROC - Joint

Joint Air-to-Ground Missile (JAGM)

DTIC Science & Technology

2015-12-01

Selected Acquisition Report ( SAR ) RCS: DD-A&T(Q&A)823-355 Joint Air-to-Ground Missile (JAGM) As of FY 2017 President’s Budget Defense Acquisition...Management Information Retrieval (DAMIR) March 4, 2016 09:30:05 UNCLASSIFIED JAGM December 2015 SAR March 4, 2016 09:30:05 UNCLASSIFIED 2 Table of...Office of the Secretary of Defense O&S - Operating and Support PAUC - Program Acquisition Unit Cost JAGM December 2015 SAR March 4, 2016 09:30:05

78 FR 26665 - Excepted Service

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-07

... Organization name Position title No. Effective date Department of Commerce Office of White House Deputy... Assistant for DD130034 2/8/2013 Assistant Secretary Middle East Affairs. of Defense (International Security... Transportation...... Assistant Secretary Associate Director DT130011 2/27/2013 for Governmental for Governmental...

Differential phosphorylation of azidothymidine, dideoxycytidine, and dideoxyinosine in resting and activated peripheral blood mononuclear cells.

PubMed Central

Gao, W Y; Shirasaka, T; Johns, D G; Broder, S; Mitsuya, H

1993-01-01

The antiviral activity of azidothymidine (AZT), dideoxycytidine (ddC), and dideoxyinosine (ddI) against HIV-1 was comparatively evaluated in PHA-stimulated PBM. The mean drug concentration which yielded 50% p24 Gag negative cultures were substantially different: 0.06, 0.2, and 6 microM for AZT, ddC, and ddI, respectively. We found that AZT was preferentially phosphorylated to its triphosphate (TP) form in PHA-PBM rather than unstimulated, resting PBM (R-PBM), producing 10- to 17-fold higher ratios of AZTTP/dTTP in PHA-PBM than in R-PBM. The phosphorylation of ddC and ddI to their TP forms was, however, much less efficient in PHA-PBM, resulting in approximately 5-fold and approximately 15-fold lower ratios of ddCTP/dCTP and ddATP/dATP, respectively, in PHA-PBM than in R-PBM. The comparative order of PHA-induced increase in cellular enzyme activities examined was: thymidine kinase > uridine kinase > deoxycytidine kinase > adenosine kinase > 5'-nucleotidase. We conclude that AZT, ddC, and ddI exert disproportionate antiviral effects depending on the activation state of the target cells, i.e., ddI and ddC exert antiviral activity more favorably in resting cells than in activated cells, while AZT preferentially protects activated cells against HIV infection. Considering that HIV-1 proviral DNA synthesis in resting lymphocytes is reportedly initiated at levels comparable with those of activated lymphocytes, the current data should have practical relevance in the design of anti-HIV chemotherapy, particularly combination chemotherapy. PMID:8387546

75 FR 60423 - Submission for OMB Review; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-30

... November 1, 2010. Title and OMB Number: DoD Building Pass Application; DD Form 2249; OMB Number 0704-0328... applicants for DoD Building Passes. The information collected from the DD Form 2249, ``DoD Building Pass...

AIM-9X Block II Sidewinder (AIM-9X Blk II)

DTIC Science & Technology

2015-12-01

Selected Acquisition Report (SAR) RCS: DD- A &T(Q& A )823-442 AIM-9X Block II Sidewinder (AIM-9X Blk II) As of FY 2017 President’s Budget Defense...MDAP - Major Defense Acquisition Program MILCON - Military Construction N/ A - Not Applicable O&M - Operations and Maintenance ORD - Operational...15:14:10 UNCLASSIFIED 5 Mission and Description The AIM-9X Block II Sidewinder (AIM-9X Blk II) short-range air-to-air missile is a long term

KC-46A Tanker Modernization (KC-46A)

DTIC Science & Technology

2015-12-01

Selected Acquisition Report ( SAR ) RCS: DD-A&T(Q&A)823-387 KC-46A Tanker Modernization (KC-46A) As of FY 2017 President’s Budget Defense Acquisition...Management Information Retrieval (DAMIR) March 22, 2016 16:45:38 UNCLASSIFIED KC-46A December 2015 SAR March 22, 2016 16:45:38 UNCLASSIFIED 2...Document OSD - Office of the Secretary of Defense O&S - Operating and Support PAUC - Program Acquisition Unit Cost KC-46A December 2015 SAR March 22

C-130J Hercules Transport Aircraft (C-130J)

DTIC Science & Technology

2015-12-01

Selected Acquisition Report ( SAR ) RCS: DD-A&T(Q&A)823-220 C-130J Hercules Transport Aircraft (C-130J) As of FY 2017 President’s Budget Defense...Acquisition Management Information Retrieval (DAMIR) March 21, 2016 11:33:03 UNCLASSIFIED C-130J December 2015 SAR March 21, 2016 11:33:03...Requirements Document OSD - Office of the Secretary of Defense O&S - Operating and Support PAUC - Program Acquisition Unit Cost C-130J December 2015 SAR March 21

KC-130J Transport Aircraft (KC-130J)

DTIC Science & Technology

2015-12-01

Selected Acquisition Report ( SAR ) RCS: DD-A&T(Q&A)823-433 KC-130J Transport Aircraft (KC-130J) As of FY 2017 President’s Budget Defense Acquisition...Management Information Retrieval (DAMIR) March 23, 2016 15:23:28 UNCLASSIFIED KC-130J December 2015 SAR March 23, 2016 15:23:28 UNCLASSIFIED 2...Document OSD - Office of the Secretary of Defense O&S - Operating and Support PAUC - Program Acquisition Unit Cost KC-130J December 2015 SAR March 23

UH-60M Black Hawk Helicopter (UH-60M Black Hawk)

DTIC Science & Technology

2016-12-01

Selected Acquisition Report (SAR) RCS: DD-A&T(Q&A)823-341 UH-60M Black Hawk Helicopter (UH-60M Black Hawk) As of FY 2017 President’s Budget Defense...Acquisition Management Information Retrieval (DAMIR) March 21, 2016 18:25:45 UNCLASSIFIED UH-60M Black Hawk December 2015 SAR March 21, 2016 18...Operational Requirements Document OSD - Office of the Secretary of Defense O&S - Operating and Support PAUC - Program Acquisition Unit Cost UH-60M Black Hawk

32 CFR Table 1 to Part 855 - Purpose of Use/Verification/Approval Authority/Fees

Code of Federal Regulations, 2011 CFR

2011-07-01

... change of station, etc.) or for private, non revenue flights Social security number in block 1 on DD Form... of a uniformed service member Identification card (DD Form 1173) number or social security number... Form 1173) number or social security number, identification card expiration date, sponsor's retirement...

32 CFR Table 1 to Part 855 - Purpose of Use/Verification/Approval Authority/Fees

Code of Federal Regulations, 2013 CFR

2013-07-01

... change of station, etc.) or for private, non revenue flights Social security number in block 1 on DD Form... of a uniformed service member Identification card (DD Form 1173) number or social security number... Form 1173) number or social security number, identification card expiration date, sponsor's retirement...

32 CFR 724.502 - Actions to be taken by the applicant preliminary to discharge review.

Code of Federal Regulations, 2012 CFR

2012-07-01

... the United States, DD Form 293 must be used in requesting a discharge review. DD Form 293 is available at most military installations and regional offices of the Veterans Administration. This form is to... incompetent, the form must be signed by an authorized individual as discussed in § 724.113 of this Manual. (b...

32 CFR 724.502 - Actions to be taken by the applicant preliminary to discharge review.

Code of Federal Regulations, 2014 CFR

2014-07-01

... the United States, DD Form 293 must be used in requesting a discharge review. DD Form 293 is available at most military installations and regional offices of the Veterans Administration. This form is to... incompetent, the form must be signed by an authorized individual as discussed in § 724.113 of this Manual. (b...

32 CFR 724.502 - Actions to be taken by the applicant preliminary to discharge review.

Code of Federal Regulations, 2013 CFR

2013-07-01

... the United States, DD Form 293 must be used in requesting a discharge review. DD Form 293 is available at most military installations and regional offices of the Veterans Administration. This form is to... incompetent, the form must be signed by an authorized individual as discussed in § 724.113 of this Manual. (b...

32 CFR 724.502 - Actions to be taken by the applicant preliminary to discharge review.

Code of Federal Regulations, 2010 CFR

2010-07-01

... the United States, DD Form 293 must be used in requesting a discharge review. DD Form 293 is available at most military installations and regional offices of the Veterans Administration. This form is to... incompetent, the form must be signed by an authorized individual as discussed in § 724.113 of this Manual. (b...

32 CFR 724.502 - Actions to be taken by the applicant preliminary to discharge review.

Code of Federal Regulations, 2011 CFR

2011-07-01

... the United States, DD Form 293 must be used in requesting a discharge review. DD Form 293 is available at most military installations and regional offices of the Veterans Administration. This form is to... incompetent, the form must be signed by an authorized individual as discussed in § 724.113 of this Manual. (b...

32 CFR 286.33 - Reports control.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 2 2010-07-01 2010-07-01 false Reports control. 286.33 Section 286.33 National... INFORMATION ACT PROGRAM DOD FREEDOM OF INFORMATION ACT PROGRAM REGULATION Reports § 286.33 Reports control. (a....1(a). (3) The reporting requirement outlined in this subpart is assigned Report Control Symbol DD-DA...

32 CFR 286.33 - Reports control.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 2 2014-07-01 2014-07-01 false Reports control. 286.33 Section 286.33 National... INFORMATION ACT PROGRAM DOD FREEDOM OF INFORMATION ACT PROGRAM REGULATION Reports § 286.33 Reports control. (a....1(a). (3) The reporting requirement outlined in this subpart is assigned Report Control Symbol DD-DA...

48 CFR 215.404-71-4 - Facilities capital employed.

Code of Federal Regulations, 2013 CFR

2013-10-01

... the facilities capital cost of money and capital employed using— (1) An analysis of the appropriate Forms CASB-CMF and cost of money factors (48 CFR 9904.414 and FAR 31.205-10); and (2) DD Form 1861, Contract Facilities Capital Cost of Money. (c) Use of DD Form 1861. See PGI 215.404-71-4(c) for obtaining...

48 CFR 215.404-71-4 - Facilities capital employed.

Code of Federal Regulations, 2012 CFR

2012-10-01

... the facilities capital cost of money and capital employed using— (1) An analysis of the appropriate Forms CASB-CMF and cost of money factors (48 CFR 9904.414 and FAR 31.205-10); and (2) DD Form 1861, Contract Facilities Capital Cost of Money. (c) Use of DD Form 1861. See PGI 215.404-71-4(c) for obtaining...

48 CFR 215.404-71-4 - Facilities capital employed.

Code of Federal Regulations, 2014 CFR

2014-10-01

... the facilities capital cost of money and capital employed using— (1) An analysis of the appropriate Forms CASB-CMF and cost of money factors (48 CFR 9904.414 and FAR 31.205-10); and (2) DD Form 1861, Contract Facilities Capital Cost of Money. (c) Use of DD Form 1861. See PGI 215.404-71-4(c) for obtaining...

Neural circuit rewiring: insights from DD synapse remodeling.

PubMed

Kurup, Naina; Jin, Yishi

2016-01-01

Nervous systems exhibit many forms of neuronal plasticity during growth, learning and memory consolidation, as well as in response to injury. Such plasticity can occur across entire nervous systems as with the case of insect metamorphosis, in individual classes of neurons, or even at the level of a single neuron. A striking example of neuronal plasticity in C. elegans is the synaptic rewiring of the GABAergic Dorsal D-type motor neurons during larval development, termed DD remodeling. DD remodeling entails multi-step coordination to concurrently eliminate pre-existing synapses and form new synapses on different neurites, without changing the overall morphology of the neuron. This mini-review focuses on recent advances in understanding the cellular and molecular mechanisms driving DD remodeling.

Structural Insights into DD-Fold Assembly and Caspase-9 Activation by the Apaf-1 Apoptosome.

PubMed

Su, Tsung-Wei; Yang, Chao-Yu; Kao, Wen-Pin; Kuo, Bai-Jiun; Lin, Shan-Meng; Lin, Jung-Yaw; Lo, Yu-Chih; Lin, Su-Chang

2017-03-07

Death domain (DD)-fold assemblies play a crucial role in regulating the signaling to cell survival or death. Here we report the crystal structure of the caspase recruitment domain (CARD)-CARD disk of the human apoptosome. The structure surprisingly reveals that three 1:1 Apaf-1:procaspase-9 CARD protomers form a novel helical DD-fold assembly on the heptameric wheel-like platform of the apoptosome. The small-angle X-ray scattering and multi-angle light scattering data also support that three protomers could form an oligomeric complex similar to the crystal structure. Interestingly, the quasi-equivalent environment of CARDs could generate different quaternary CARD assemblies. We also found that the type II interaction is conserved in all DD-fold complexes, whereas the type I interaction is found only in the helical DD-fold assemblies. This study provides crucial insights into the caspase activation mechanism, which is tightly controlled by a sophisticated and highly evolved CARD assembly on the apoptosome, and also enables better understanding of the intricate DD-fold assembly. Copyright © 2017 Elsevier Ltd. All rights reserved.

Incorporating TRMM and Other High-Quality Estimates into the One-Degree Daily (1DD) Global Precipitation Product

NASA Technical Reports Server (NTRS)

Huffman, George J.; Adler, Robert F.; Bolvin, David T.

1999-01-01

The One-Degree Daily (1DD) precipitation dataset was recently developed for the Global Precipitation Climatology Project (GPCP). The IDD provides a globally-complete, observation-only estimate of precipitation on a daily 1 deg x 1 deg grid for the period 1997 through late 1999 (by the time of the conference). In the latitude band 40 N - 40 S the IDD uses the Threshold-Matched Precipitation Index (TMPI), a GPI-like IR product with the T(sub b) threshold and (single) conditional rain rate determined locally for each month by the frequency of precipitation in the GPROF SSNU product and by the precipitation amount in the GPCP satellite-gauge (SG) combination. Outside 40 N - 40 S the 1DD uses a scaled TOVS precipitation estimate that has adjustments based on the TMPI and the SG. This first-generation 1DD has been in beta test preparatory to release as an official GPCP product. In this paper we discuss further development of the 1DD framework to allow the direct incorporation of TRMM and other high-quality precipitation estimates. First, these data are generally sparse (typically from low-orbit satellites), so a fair amount of work was devoted to data boundaries. Second, these data are not the same as the original 1DD estimates, so we had to give careful consideration to the best scheme for forcing the 1DD to sum to the SG for the month. Finally, the non-sun-synchronous, low-inclination orbit occupied by TRMM creates interesting variations against the sun-synchronous, high-inclination orbits occupied by the Defense Meteorological Satellite Program satellites that carry the SSM/I. Examples will be given of each of the development issues, then comparisons will be made to daily raingauge analyses.

75 FR 81238 - Submission for OMB Review; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-27

... correction of any deficiencies. Affected Public: Business or other for-profit. Frequency: On Occasion... Supplement (DFARS) Subpart 215.4, Contract Pricing; DD Form 1861, Contract Facilities Capital Cost of Money... Hours: 538,480 hours. Needs and Uses: DoD contracting officers use DD Form 1861 in computing profit...

48 CFR 1846.672 - Preparing DD Forms 250 and 250c.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Preparing DD Forms 250 and 250c. 1846.672 Section 1846.672 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE ADMINISTRATION CONTRACT MANAGEMENT QUALITY ASSURANCE Material Inspection and Receiving Reports 1846.672 Preparing...

32 CFR 728.33 - Nonavailability statement (DD 1251).

Code of Federal Regulations, 2011 CFR

2011-07-01

... considered part of the initial reason for admission of the mother (delivery), and is therefore considered a... instructions on the reverse of the form. After completion, if authorized by the facility CO, the issuing... form. DD 1251's issued under the CO's discretionary authority for the “medically inappropriate reason...

32 CFR 728.33 - Nonavailability statement (DD 1251).

Code of Federal Regulations, 2010 CFR

2010-07-01

... considered part of the initial reason for admission of the mother (delivery), and is therefore considered a... instructions on the reverse of the form. After completion, if authorized by the facility CO, the issuing... form. DD 1251's issued under the CO's discretionary authority for the “medically inappropriate reason...

Genomic profiling of dedifferentiated liposarcoma compared to matched well-differentiated liposarcoma reveals higher genomic complexity and a common origin

PubMed Central

Beird, Hannah C.; Wu, Chia-Chin; Ingram, Davis R.; Wang, Wei-Lien; Alimohamed, Asrar; Gumbs, Curtis; Little, Latasha; Song, Xingzhi; Feig, Barry W.; Roland, Christina L.; Zhang, Jianhua; Benjamin, Robert S.; Hwu, Patrick; Lazar, Alexander J.; Futreal, P. Andrew; Somaiah, Neeta

2018-01-01

Well-differentiated (WD) liposarcoma is a low-grade mesenchymal tumor with features of mature adipocytes and high propensity for local recurrence. Often, WD patients present with or later progress to a higher-grade nonlipogenic form known as dedifferentiated (DD) liposarcoma. These DD tumors behave more aggressively and can metastasize. Both WD and DD liposarcomas harbor neochromosomes formed from amplifications and rearrangements of Chr 12q that encode oncogenes (MDM2, CDK4, and YEATS2) and adipocytic differentiation factors (HMGA2 and CPM). However, genomic changes associated with progression from WD to DD have not been well-defined. Therefore, we selected patients with matched WD and DD tumors for extensive genomic profiling in order to understand their clonal relationships and to delineate any defining alterations for each entity. Exome and transcriptomic sequencing was performed for 17 patients with both WD and DD diagnoses. Somatic point and copy-number alterations were integrated with transcriptional analyses to determine subtype-associated genomic features and pathways. The results were, on average, that only 8.3% of somatic mutations in WD liposarcoma were shared with their cognate DD component. DD tumors had higher numbers of somatic copy-number losses, amplifications involving Chr 12q, and fusion transcripts than WD tumors. HMGA2 and CPM rearrangements occur more frequently in DD components. The shared somatic mutations indicate a clonal origin for matched WD and DD tumors and show early divergence with ongoing genomic instability due to continual generation and selection of neochromosomes. Stochastic generation and subsequent expression of fusion transcripts from the neochromosome that involve adipogenesis genes such as HMGA2 and CPM may influence the differentiation state of the subsequent tumor. PMID:29610390

Trip Report of Field Search for Exercise Desert Rock Documentation Conducted by Representatives of The Adjutant General 18 June 1978 to 14 July 1978

DTIC Science & Technology

1978-08-04

flip. 3. Instructions for completing forms.-DD Form 7A1 I nd DD) Form 742 -ire used by trained examiners andl the itemns ictluiring comt - pletion are...radiation exposure report by organization for July. Monthly radiation exposure report by organization for Agust . Monthly radiation exposure report by

32 CFR 701.52 - Computation of fees.

Code of Federal Regulations, 2010 CFR

2010-07-01

... correspondence and preparation costs, these fees are not recoupable from the requester. (b) DD 2086, Record of... costs, as requesters may solicit a copy of that document to ensure accurate computation of fees. Costs... 32 National Defense 5 2010-07-01 2010-07-01 false Computation of fees. 701.52 Section 701.52...

78 FR 69273 - Defense Federal Acquisition Regulation Supplement: Safeguarding Unclassified Controlled Technical...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-18

... list is added specifying the 13 pieces of information required for reporting. The time period a..., DoD 5000 series, DoD 8570.01-M, Directives (DoDD), National Industrial Security Operating Manual... on the scope of the proposed DFARS section 204.7400 including: university fundamental research should...

32 CFR 518.22 - Reports control.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 3 2013-07-01 2013-07-01 false Reports control. 518.22 Section 518.22 National... RELATIONS THE FREEDOM OF INFORMATION ACT PROGRAM Reports § 518.22 Reports control. (a) General. (1) The... requirement outlined is assigned Report Control Symbol DD-DA&M(A)1365, FOIA Report to Congress. (b) Reporting...

32 CFR 518.22 - Reports control.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 3 2012-07-01 2009-07-01 true Reports control. 518.22 Section 518.22 National... RELATIONS THE FREEDOM OF INFORMATION ACT PROGRAM Reports § 518.22 Reports control. (a) General. (1) The... requirement outlined is assigned Report Control Symbol DD-DA&M(A)1365, FOIA Report to Congress. (b) Reporting...

32 CFR 518.22 - Reports control.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 3 2010-07-01 2010-07-01 true Reports control. 518.22 Section 518.22 National... RELATIONS THE FREEDOM OF INFORMATION ACT PROGRAM Reports § 518.22 Reports control. (a) General. (1) The... requirement outlined is assigned Report Control Symbol DD-DA&M(A)1365, FOIA Report to Congress. (b) Reporting...

32 CFR 518.22 - Reports control.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 3 2014-07-01 2014-07-01 false Reports control. 518.22 Section 518.22 National... RELATIONS THE FREEDOM OF INFORMATION ACT PROGRAM Reports § 518.22 Reports control. (a) General. (1) The... requirement outlined is assigned Report Control Symbol DD-DA&M(A)1365, FOIA Report to Congress. (b) Reporting...

32 CFR 518.22 - Reports control.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 3 2011-07-01 2009-07-01 true Reports control. 518.22 Section 518.22 National... RELATIONS THE FREEDOM OF INFORMATION ACT PROGRAM Reports § 518.22 Reports control. (a) General. (1) The... requirement outlined is assigned Report Control Symbol DD-DA&M(A)1365, FOIA Report to Congress. (b) Reporting...

Functional Characterisation of the WW Minimal Domain for Delivering Therapeutic Proteins by Adenovirus Dodecahedron

PubMed Central

Villegas-Méndez, Ana; Fender, Pascal; Garin, Marina I.; Rothe, Romy; Liguori, Lavinia; Marques, Bruno; Lenormand, Jean-Luc

2012-01-01

Protein transduction offers a great therapeutic potential by efficient delivery of biologically active cargo into cells. The Adenovirus Dd (Dodecahedron) has recently been shown to deliver proteins fused to the tandem WW2-3-4 structural domains from the E3 ubiquitin ligase Nedd4. In this study, we conclusively show that Dd is able to efficiently deliver cargo inside living cells, which mainly localize in fast moving endocytic vesicles, supporting active transport along the cytoskeleton. We further improve this delivery system by expressing a panel of 13 WW-GFP mutant forms to characterize their binding properties towards Dd. We identified the domain WW3 and its mutant form WW3_10_13 to be sufficient for optimal binding to Dd. We greatly minimise the interacting WW modules from 20 to 6 kDa without compromising its efficient delivery by Dd. Using these minimal WW domains fused to the tumor suppressor p53 protein, we show efficient cellular uptake and distribution into cancer cells, leading to specific induction of apoptosis in these cells. Taken together, these findings represent a step further towards the development of a Dd-based delivery system for future therapeutic application. PMID:23028993

Evidence that noncoding RNA dutA is a multicopy suppressor of Dictyostelium discoideum STAT protein Dd-STATa.

PubMed

Shimada, Nao; Kawata, Takefumi

2007-06-01

Dd-STATa, a Dictyostelium discoideum homologue of metazoan STAT transcription factors, is necessary for culmination. We created a mutant strain with partial Dd-STATa activity and used it to screen for unlinked suppressor genes. We screened approximately 450,000 clones from a slug-stage cDNA library for their ability to rescue the culmination defect when overexpressed. There were 12 multicopy suppressors of Dd-STATa, of which 4 encoded segments of a known noncoding RNA, dutA. Expression of dutA is specific to the pstA zone, the region where Dd-STATa is activated. In suppressed strains the expression patterns of several putative Dd-STATa target genes become similar to the wild-type strain. In addition, the amount of the tyrosine-phosphorylated form of Dd-STATa is significantly increased in the suppressed strain. These results indicate that partial copies of dutA may act upstream of Dd-STATa to regulate tyrosine phosphorylation by an unknown mechanism.

Evidence that Noncoding RNA dutA Is a Multicopy Suppressor of Dictyostelium discoideum STAT Protein Dd-STATa▿

PubMed Central

Shimada, Nao; Kawata, Takefumi

2007-01-01

Dd-STATa, a Dictyostelium discoideum homologue of metazoan STAT transcription factors, is necessary for culmination. We created a mutant strain with partial Dd-STATa activity and used it to screen for unlinked suppressor genes. We screened approximately 450,000 clones from a slug-stage cDNA library for their ability to rescue the culmination defect when overexpressed. There were 12 multicopy suppressors of Dd-STATa, of which 4 encoded segments of a known noncoding RNA, dutA. Expression of dutA is specific to the pstA zone, the region where Dd-STATa is activated. In suppressed strains the expression patterns of several putative Dd-STATa target genes become similar to the wild-type strain. In addition, the amount of the tyrosine-phosphorylated form of Dd-STATa is significantly increased in the suppressed strain. These results indicate that partial copies of dutA may act upstream of Dd-STATa to regulate tyrosine phosphorylation by an unknown mechanism. PMID:17435008

32 CFR Table 1 to Part 855 - Purpose of Use/Verification/Approval Authority/Fees

Code of Federal Regulations, 2014 CFR

2014-07-01

... change of station, etc.) or for private, non revenue flights Social security number in block 1 on DD Form... other available aircraft for official Government business travel Supervisor's endorsement in block 4 of... be listed in block 4 of the DD Form 2401. An official government document, such as an SF 1169, US...

32 CFR Table 1 to Part 855 - Purpose of Use/Verification/Approval Authority/Fees

Code of Federal Regulations, 2012 CFR

2012-07-01

... change of station, etc.) or for private, non revenue flights Social security number in block 1 on DD Form... other available aircraft for official Government business travel Supervisor's endorsement in block 4 of... be listed in block 4 of the DD Form 2401. An official government document, such as an SF 1169, US...

32 CFR Table 1 to Part 855 - Purpose of Use/Verification/Approval Authority/Fees

Code of Federal Regulations, 2010 CFR

2010-07-01

... change of station, etc.) or for private, non revenue flights Social security number in block 1 on DD Form... other available aircraft for official Government business travel Supervisor's endorsement in block 4 of... be listed in block 4 of the DD Form 2401. An official government document, such as an SF 1169, US...

78 FR 59341 - Submission for OMB Review; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-26

...: Title, Associated Form and OMB Number: TRICARE Plus Enrollment Application (DD 2853) and TRICARE Plus Disenrollment Request (DD 2854); OMB Control Number 0720-0028. Type of Request: Reinstatement with Change Number...

Customs Inspection

DTIC Science & Technology

1977-05-01

accordance with DA Form 12 -9A require- ments for Transportation and Travel. Active Army: B ARNG: D A Ision For USAR: D . A&I Air Force: F DTIU TAB &0...Personal Property Shipments (DD Form 1252) ------- 8- 12 . 1 8-4 DOD Catalytic Converter Import Control Label (DD Form 2023) ----------------- 8-13 *8-5...and ie nr notecsostrioyo hchelick foperty himerathed tom e ha shger id United States, including controlled substances,checked for him /her at the tim e

75 FR 60424 - Submission for OMB Review; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-30

.... Title and OMB Number: Appointment of Chaplains for the Military Services; DD Form 2088; OMB Control... Professional is professionally qualified to become a chaplain. The DD 2088 is used to verify the professional...

76 FR 50771 - Submission for Review: DD 1918 Establishment Information Form, DD 1919 Wage Data Collection Form...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-16

... Personnel Management, Attention: Jerome D. Mikowicz, Deputy Associate Director for Pay and Leave, 1900 E Street, NW., Room 7H31, Washington, DC 20415- 8200, or sent via electronic mail to pay[email protected] documentation, may be obtained by contacting the U.S. Office of Personnel Management, Attention: Jerome D...

Two different streams form the dorsal visual system: anatomy and functions.

PubMed

Rizzolatti, Giacomo; Matelli, Massimo

2003-11-01

There are two radically different views on the functional role of the dorsal visual stream. One considers it as a system involved in space perception. The other is of a system that codes visual information for action organization. On the basis of new anatomical data and a reconsideration of previous functional and clinical data, we propose that the dorsal stream and its recipient parietal areas form two distinct functional systems: the dorso-dorsal stream (d-d stream) and the ventro-dorsal stream (v-d stream). The d-d stream is formed by area V6 (main d-d extrastriate visual node) and areas V6A and MIP of the superior parietal lobule. Its major functional role is the control of actions "on line". Its damage leads to optic ataxia. The v-d stream is formed by area MT (main v-d extrastriate visual node) and by the visual areas of the inferior parietal lobule. As the d-d stream, v-d stream is responsible for action organization. It, however, also plays a crucial role in space perception and action understanding. The putative mechanisms linking action and perception in the v-d stream is discussed.

Evidence for a functional link between Dd-STATa and Dd-PIAS, a Dictyostelium PIAS homologue.

PubMed

Kawata, Takefumi; Hirano, Tatsunori; Ogasawara, Shun; Aoshima, Ryota; Yachi, Ayako

2011-09-01

Several mammalian protein families inhibit the activity of signal transducer and activator of transcription (STAT) proteins. The protein inhibitor of activated STAT (PIAS) was initially identified through its ability to interact with human STAT proteins. We isolated a gene (pisA) encoding a Dictyostelium orthologue of PIAS, Dd-PIAS, which possesses almost all the representative motifs and domains of mammalian PIAS proteins. A Dd-PIAS null mutant strain displays a normal terminal morphology but with accelerated development once cells are aggregated. In contrast, Dd-PIAS overexpressor strains demonstrate delayed aggregation, almost no slug phototaxis, impaired slug motility, and a prolonged slug migration period. This strain is a near phenocopy of the Dd-STATa null mutant, although it eventually forms a fruiting body, albeit inefficiently. The expression of several Dd-STATa-activated genes is upregulated in the Dd-PIAS null mutant and there is ectopic expression of pstAB makers. The concentration of a PIAS-green fluorescent protein (GFP) fusion protein, expressed under the PIAS promoter, is greatest in the pstO cells and gradually decreases with proximity to the tip of the slug and culminant: a pattern diametrically opposite to that of Dd-STATa. Our results suggest a functional interrelationship between Dd-PIAS and Dd-STATa that influences gene expression and development. © 2011 The Authors. Development, Growth & Differentiation © 2011 Japanese Society of Developmental Biologists.

48 CFR 215.404-72 - Modified weighted guidelines method for nonprofit organizations other than FFRDCs.

Code of Federal Regulations, 2010 CFR

2010-10-01

...) Do not assign a value from the technology incentive designated range. (2) Modifications to contract type risk (Block 24 of the DD Form 1547). Use a designated range of −1 percent to 0 percent instead of... performance risk (Blocks 21-23 of the DD Form 1547). (i) If the contracting officer assigns a value from the...

Delusional disorder and schizophrenia: a comparative study across multiple domains.

PubMed

Peralta, V; Cuesta, M J

2016-10-01

Delusional disorder (DD) is an under-researched condition and its relationship to schizophrenia (SZ) controversial. This study aimed to further characterize DD and to examine multi-domain evidence for the distinction between DD and SZ. Using univariate analyses we examined 146 subjects with DD, 114 subjects with paranoid SZ and 244 subjects with non-paranoid SZ on 52 characteristics from several domains including demographics, risk factors, premorbid features, illness characteristics, index episode features, delusional-related features, response to treatment and outcome. In a further step, we searched for independent associations of the examined characteristics with DD v. SZ. Univariate analyses showed that DD differed from either form of SZ in 40 characteristics, the pattern of findings indicated that paranoid SZ was much more similar to non-paranoid SZ than DD. Relative to subjects with SZ, those with DD were more likely to have drug abuse before illness onset, better premorbid sexual adjustment, later age at illness onset, higher levels of affective symptoms and lack of insight, poorer response to antipsychotic medication, better functioning in the domains of personal care, paid work and social functioning; last, subjects with DD had fewer but more severe delusions and higher ratings of conviction of delusional experience than those with SZ. Predominance of jealousy and somatic delusions was confined to subjects with DD. DD and SZ represent two distinct classes of disorders, the differential features of DD being of nosological, aetiological and therapeutic relevance.

Littoral Combat Ship (LCS)

DTIC Science & Technology

2015-12-01

Selected Acquisition Report ( SAR ) RCS: DD-A&T(Q&A)823-374 Littoral Combat Ship (LCS) As of FY 2017 President’s Budget Defense Acquisition...Management Information Retrieval (DAMIR) UNCLASSIFIED LCS December 2015 SAR UNCLASSIFIED 2 Table of Contents Common Acronyms and Abbreviations for...and Support PAUC - Program Acquisition Unit Cost LCS December 2015 SAR UNCLASSIFIED 3 PB - President’s Budget PE - Program Element PEO - Program

Chronic complex dissociative disorders and borderline personality disorder: disorders of emotion dysregulation?

PubMed

Brand, Bethany L; Lanius, Ruth A

2014-01-01

Emotion dysregulation is a core feature of chronic complex dissociative disorders (DD), as it is for borderline personality disorder (BPD). Chronic complex DD include dissociative identity disorder (DID) and the most common form of dissociative disorder not otherwise specified (DDNOS, type 1), now known as Other Specified Dissociative Disorders (OSDD, type 1). BPD is a common comorbid disorder with DD, although preliminary research indicates the disorders have some distinguishing features as well as considerable overlap. This article focuses on the epidemiology, clinical presentation, psychological profile, treatment, and neurobiology of chronic complex DD with emphasis placed on the role of emotion dysregulation in each of these areas. Trauma experts conceptualize borderline symptoms as often being trauma based, as are chronic complex DD. We review the preliminary research that compares DD to BPD in the hopes that this will stimulate additional comparative research.

Density Dependent Functional Forms Drive Compensation in Populations Exposed to Stressors

EPA Science Inventory

The interaction between density dependence (DD) and environmental stressors can result in a compensatory or synergistic response in population growth, and population models that use density-independent demographic rates or generic DD functions may be introducing bias into managem...

ADVANCEMENTS IN NEUTRON RADIOGRAPHY WITHIN THE DEPARTMENT OF THE ARMY

DTIC Science & Technology

2016-11-01

Defense (DoD) production facility for direct use in quality control of munitions and weapons system by means of neutron radiographic inspection...UNCLASSIFIED UNCLASSIFIED AD-E403 813 Technical Report AREIS-TR-16004 ADVANCEMENTS IN NEUTRON RADIOGRAPHY WITHIN THE...REPORT DATE (DD-MM-YYYY) November 2016 2. REPORT TYPE Final 3. DATES COVERED (From – To) 4. TITLE AND SUBTITLE ADVANCEMENTS IN NEUTRON

Comparison of Biocorrosion due to Desulfovibrio desulfuricans and Desulfotomaculum nigrificans Bacteria

NASA Astrophysics Data System (ADS)

Lata, Suman; Sharma, Chhaya; Singh, Ajay K.

2013-02-01

One observes several species of sulfate-reducing bacteria in nature. Presence of these species in a media may cause microbial influenced corrosion (MIC) of materials differently. To investigate this aspect of MIC, corrosion tests were performed on three types of stainless steels. The tests were done in modified Baar's media inoculated separately by the two species of SRB namely Desulfovibrio desulfuricans (DD) and Desulfotomaculum nigrificans (DN). Electrochemical and immersion tests were performed to assess the extent of uniform and localized corrosion of these steels. Biofilms formed on the corroded samples were analyzed for estimating various components of its extracellular polymeric substances. Hydrogenase enzyme of these bacteria was tested to determine its nature and activity. Higher degree of corrosivity was observed in case of media inoculated with DD as compared to DN. More active nature of hydrogenase enzyme, its location in the periplasmic phase in DD and higher fraction of carbohydrate in biofilm formed due to DD have been suggested to be responsible for higher degree of corrosivity caused by them.

Spectroscopic studies on [(DD18C6H 2)(HPA) 2](PA) 2 and [(DD18C6H 2)(DDQ) 2](DDQH) 2 formed in the reaction of N, N'-dibenzyl-1,4,10,13-tetraoxa-7,16-diazacyclooctadecane with HPA and DDQ

NASA Astrophysics Data System (ADS)

Teleb, Said M.; Gaballa, Akmal S.; Elmosallamy, M. A. F.; Nour, El-Metwally

2005-09-01

The interaction of the mixed oxygen-nitrogen cyclic base, N, N'-dibenzyl-1,4,10,13-tetraoxa-7,16-diazacyclooctadecane (DD18C6) with π-acceptors such as picric acid (HPA) and 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) has been studied spectrophotometrically in chloroform at 25 °C. The results obtained indicate the formation of 1:4 charge-transfer complexes with the general formula (DD18C6)(acceptor) 4. The electronic and infrared spectra of charge-transfer complexes along with the 1H NMR spectra were recorded and discussed. Based on the data obtained, the complexes were formulated as [(DD18C6H 2)(HPA) 2](PA) 2 and [(DD18C6H 2)(DDQ) 2](DDQH) 2. A general mechanism explaining the formation of the DDQ complex has been suggested.

Epilepsy and Intellectual and Developmental Disabilities

ERIC Educational Resources Information Center

Oguni, Hirokazu

2013-01-01

The co-occurrence of epilepsy in people with intellectual disabilities (ID) and other developmental disabilities (DD) has received attention because it has a significant negative impact on health, well-being, and quality of life. The current research investigating the frequency and form of epilepsy in children with ID and DD is reviewed, with…

Is deep dreaming the new collage?

NASA Astrophysics Data System (ADS)

Boden, Margaret A.

2017-10-01

Deep dreaming (DD) can combine and transform images in surprising ways. But, being based in deep learning (DL), it is not analytically understood. Collage is an art form that is constrained along various dimensions. DD will not be able to generate collages until DL can be guided in a disciplined fashion.

Oxidative stress and toxicity induced by the nucleoside reverse transcriptase inhibitor (NRTI)--2',3'-dideoxycytidine (ddC): relevance to HIV-dementia.

PubMed

Opii, Wycliffe O; Sultana, Rukhsana; Abdul, Hafiz Mohmmad; Ansari, Mubeen Ahmad; Nath, Avindra; Butterfield, D Allan

2007-03-01

Human immunodeficiency virus dementia (HIVD) is the most common form of dementia occurring among young adults. In HIVD, neuronal cell loss occurs in the absence of neuronal infection. With the advent of highly active anti-retroviral therapy (HAART), the incidence of HIVD has drastically reduced, though prevalence of milder forms of HIVD continues to rise. Though these agents have been used successfully in suppressing viral production, they have also been associated with a number of side effects. Here we examine the possible role of NRTIs, in particular 2',3'-dideoxycytidine (ddC), in the neuropathology of HIVD. Synaptosomes and isolated mitochondria treated and incubated for 6 h with CSF-achievable concentrations of ddC, i.e., 6-11 ng/ml, were found to show a significant increase in oxidative stress with 40 nM ddC as measured by protein carbonyls and 3-nitrotyrosine (3NT), effects that were not observed in the more tolerable NRTI, 3TC. Protection against protein oxidation induced by ddC was observed when brain mitochondria were isolated from gerbils 1 h after injection i.p. with the brain accessible antioxidant and glutathione mimetic, tricyclodecan-9-yl-xanthogenate (D609). In addition, there is a significant reduction in the levels of anti-apoptotic protein Bcl-2 and a significant increase in cytochrome c release and also a significant increase in the expression of pro-apoptotic protein caspase-3 after mitochondria were treated with 40 nM ddC. The results reported here show that ddC at 40 nM can induce oxidative stress, cause the release of cytochrome c, and in addition, reduce the levels of anti-apoptotic proteins, increase the levels of pro-apoptotic proteins, thereby increasing the possibility for induction of apoptosis. These findings are consistent with the notion of a possible role of the NRTIs, and in particular, ddC, in the mechanisms involved in HIVD.

Excalibur Precision 155mm Projectiles (Excalibur)

DTIC Science & Technology

2015-12-01

Selected Acquisition Report ( SAR ) RCS: DD-A&T(Q&A)823-366 Excalibur Precision 155mm Projectiles (Excalibur) As of FY 2017 President’s Budget...Defense Acquisition Management Information Retrieval (DAMIR) March 21, 2016 18:18:38 UNCLASSIFIED Excalibur December 2015 SAR March 21, 2016 18:18:38... SAR March 21, 2016 18:18:38 UNCLASSIFIED 3 PB - President’s Budget PE - Program Element PEO - Program Executive Officer PM - Program Manager POE

Removing Hazardous Materials from Buildings: A Training Curriculum

DTIC Science & Technology

2016-03-01

Army Pamphlet DAIM-ODF OACSIM Facility Policy Division DHHS Department of Health and Human Services DoD Department of Defense DoDD Department of...materials have the potential to: – Damage human health and safety – Damage environmental systems through releases to the air, water, or soil • Certain...EPA, or otherwise identified as creating an environmental and/or human health hazard when deteriorated or disturbed, or when entering the waste

Quick Reaction Report on DoD Procurements Through the Tennessee Valley Authority

DTIC Science & Technology

1992-04-03

from non-DoD agencies that includes sections to be completed and signed by a contracting officer. Recommendations for Corrective Actions We recommend...in the DD 350 Individual Contract Action Reporting System. The Assistant Secretary did not provide planned dates for completing the corrective actions ...will be reprocured. Tentative milestones for completion of the corrective actions were not identified. Defense Logistics Agency comments. The Deputy

Oral assessment of children with an autism spectrum disorder.

PubMed

DeMattei, R; Cuvo, A; Maurizio, S

2007-01-01

The study assessed the oral health status of children with an autism spectrum disorder (ASD) to help establish the oral health needs of this population. Oral assessments were conducted on 39 children with an ASD and 16 children with other developmental disabilities (DD), solicited from 3 different schools. Conditions assessed were bacterial plaque, gingivitis, dental caries, restorations, bruxism, delayed eruption/missing teeth, oral infection, developmental anomalies, injuries, occlusion, salivary flow, and oral defensiveness. Chi-square and Fisher's exact test of significance were used to compare groups. Young children with an ASD who resided with parents showed significantly more signs of bruxism than the comparison groups. Likewise, older children who lived at the residential school manifested significantly more gingivitis. No other significant differences existed when age and residence were considered for children with an ASD. When comparing children with ASD to those with another DD, the latter group showed significantly more oral injuries, abnormal salivary flow, and developmental anomalies. Children with an ASD displayed the following percentages for clinically visible conditions: plaque (85%), gingivitis (62%), and caries (21%). Approximately half of the children with ASD were orally defensive. Children with an ASD appear to have oral conditions that might increase the risk of developing dental disease. The extent of risk is unclear and needs further investigation.

Why Some People Discount More than Others: Baseline Activation in the Dorsal PFC Mediates the Link between COMT Genotype and Impatient Choice

PubMed Central

Gianotti, Lorena R. R.; Figner, Bernd; Ebstein, Richard P.; Knoch, Daria

2012-01-01

Individuals differ widely in how steeply they discount future rewards. The sources of these stable individual differences in delay discounting (DD) are largely unknown. One candidate is the COMT Val158Met polymorphism, known to modulate prefrontal dopamine levels and affect DD. To identify possible neural mechanisms by which this polymorphism may contribute to stable individual DD differences, we measured 73 participants’ neural baseline activation using resting electroencephalogram (EEG). Such neural baseline activation measures are highly heritable and stable over time, thus an ideal endophenotype candidate to explain how genes may influence behavior via individual differences in neural function. After EEG-recording, participants made a series of incentive-compatible intertemporal choices to determine the steepness of their DD. We found that COMT significantly affected DD and that this effect was mediated by baseline activation level in the left dorsal prefrontal cortex (DPFC): (i) COMT had a significant effect on DD such that the number of Val alleles was positively correlated with steeper DD (higher numbers of Val alleles means greater COMT activity and thus lower dopamine levels). (ii) A whole-brain search identified a cluster in left DPFC where baseline activation was correlated with DD; lower activation was associated with steeper DD. (iii) COMT had a significant effect on the baseline activation level in this left DPFC cluster such that a higher number of Val alleles was associated with lower baseline activation. (iv) The effect of COMT on DD was explained by the mediating effect of neural baseline activation in the left DPFC cluster. Our study thus establishes baseline activation level in left DPFC as salient neural signature in the form of an endophenotype that mediates the link between COMT and DD. PMID:22586360

Enzymatic characterization of a novel bovine liver dihydrodiol dehydrogenase--reaction mechanism and bile acid dehydrogenase activity.

PubMed

Nanjo, H; Adachi, H; Morihana, S; Mizoguchi, T; Nishihara, T; Terada, T

1995-05-11

Bovine liver cytosolic dihydrodiol dehydrogenase (DD3) has been characterized by its unique dihydrodiol dehydrogenase activity for trans-benzenedihydrodiol (trans-1,2-dihydrobenzene-1,2-diol) with the highest affinity and the greatest velocity among three multiple forms of dihydrodiol dehydrogenases (DD1-DD3). It is the first time that DD3 has shown a significant dehydrogenase activity for (S)-(+)-1-indanol with low Km value (0.33 +/- 0.022 mM) and high K(cat) value (25 +/- 0.79 min-1). The investigation of the product inhibition of (S)-(+)-1-indanol with NADP+ versus 1-indanone and NADPH clearly showed that the enzymatic reaction of DD3 may follow a typical ordered Bi Bi mechanism similar to many aldo/keto reductases. Additionally, DD3 was shown to catalyze the dehydrogenation of bile acids (lithocholic acid, taurolithocholic acid and taurochenodeoxycholic acid) having no 12-hydroxy groups with low Km values (17 +/- 0.65, 33 +/- 1.9 and 890 +/- 73 microM, respectively). In contrast, DD1, 3 alpha-hydroxysteroid dehydrogenase, shows a broad substrate specificity for many bile acids with higher affinity than those of DD3. Competitive inhibition of DD3 with androsterone against dehydrogenase activity for (S)-(+)-1-indanol, trans-benzenedihydrodiol or lithocholic acid suggests that these three substrates bind to the same substrate binding site of DD3, different from the case of human liver bile acid binder/dihydrodiol dehydrogenase (Takikawa, H., Stolz, A., Sugiyama, Y., Yoshida, H., Yamamoto, M. and Kaplowitz, N. (1990) J. Biol. Chem. 265, 2132-2136). Considering the reaction mechanism, DD3 may also play an important role in bile acids metabolism as well as the detoxication of aromatic hydrocarbons.

Naval Justice School Procedure Study Guide. Revision

DTIC Science & Technology

1990-01-01

preferring charges. The preferral of charges is accomplished by the signing and swearing to charges in Block 11 on page 1 of the charge sheet (DD Form 458...by the Naval Investigative Service (NIS) or similar agency; or upon receipt of signed and sworn charges (i.e., preferred charges on DD Form 458). 2-1 4...imcludiag. if loppropriatte in tkv astolrsoa# of joistiro sod 4.. ap4.e. the preferring of such charges alt app..? to yes to be mssimed by expected

Channeling Nanoparticles for Detection and Targeted Treatment of Breast Cancerous Lesions

DTIC Science & Technology

2011-10-01

µmoles, or 16.5mg) of PEG 2000. Dimethylaminopyridine (DMAP, 0.5 molar equivalents) and 1 -Ethyl- 3 - [ 3 - dimethylaminopropyl ]carbodiimide (EDC...control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. 1 . REPORT DATE (DD-MM-YYYY) 2. REPORT TYPE 3 . DATES COVERED (From - To) 4. TITLE...OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. 1 . REPORT DATE (DD-MM-YYYY) 14-10-2011 2. REPORT TYPE Final 3 . DATES

DOE Office of Scientific and Technical Information (OSTI.GOV)

Harper, Kyle; Truong, Thanh-Tam; Magwood, Leroy

In the process of decontaminating and decommissioning (D&D) older nuclear facilities, special precautions must be taken with removable or airborne contamination. One possible strategy utilizes foams and fixatives to affix these loose contaminants. Many foams and fixatives are already commercially available, either generically or sold specifically for D&D. However, due to a lack of revelant testing in a radioactive environment, additional verification is needed to confirm that these products not only affix contamination to their surfaces, but also will function in a D&D environment. Several significant safety factors, including flammability and worker safety, can be analyzed through the process ofmore » headspace analysis, a technique that analyzes the off gas formed before or during the curing process of the foam/fixative, usually using gas chromatography-mass spectrometry (GC-MS). This process focuses on the volatile components of a chemical, which move freely between the solid/liquid form within the sample and the gaseous form in the area above the sample (the headspace). Between possibly hot conditions in a D&D situation and heat created in a foaming reaction, the volatility of many chemicals can change, and thus different gasses can be released at different times throughout the reaction. This project focused on analysis of volatile chemicals involved in the process of using foams and fixatives to identify any potential hazardous or flammable compounds.« less

Two DD-carboxypeptidases from Mycobacterium smegmatis affect cell surface properties through regulation of peptidoglycan cross-linking and glycopeptidolipids.

PubMed

Pandey, Satya Deo; Pal, Shilpa; Kumar N, Ganesh; Bansal, Ankita; Mallick, Sathi; Ghosh, Anindya S

2018-05-07

During the peptidoglycan (PG) maturation of mycobacteria, the glycan strands are interlinked by both 3-3 (between two meso-DAP) and 4-3 cross-links (between D-ala and meso-DAP), though there is a predominance (60-80%) of 3-3 cross-links. The DD-CPases act on pentapeptides to generate tetrapeptides that are used by LD-transpeptidases as substrates to form 3-3 cross-links. Therefore, DD-CPases play a crucial role in mycobacterial PG cross-link formation. However, the physiology of DD-CPases in mycobacteria is relatively unexplored. Here, we deleted two DD-CPase genes, msmeg_2433 , and msmeg_2432 , both individually and in combination, from Mycobacterium smegmatis mc 2 155. Though the single DD-CPase deletions had no significant impact on the mycobacterial physiology, many interesting functional alterations were observed in the double deletion mutant, viz. , a predominance in PG cross-link formation was shifted from 3-3 cross-links to 4-3, cell surface glycopeptidolipid (GPL) expression was reduced and susceptibility towards β-lactams and anti-tubercular agents was enhanced. Moreover, the existence of the double mutant within murine macrophages was better as compared to the parent. Interestingly, the complementation with any one of the DD-CPase genes could restore the wild-type phenotype. In a nutshell, we infer that the altered ratio of 4-3: 3-3 PG cross-links might have influenced the expression of surface GPLs, colony morphology, biofilm formation,, drug susceptibility and subsistence of the cells within macrophages. Importance The glycan strands in mycobacterial peptidoglycan (PG) are interlinked by both 3-3 and 4-3 cross-links. The DD-CPases generate tetrapeptides by acting on the pentapeptides, and LD-transpeptidases use tetrapeptides as substrates to form 3-3 cross-links. Here, we showed that simultaneous deletions of two DD-CPases alter the nature of PG cross-linking from 3-3 cross-links to 4-3 cross-links. The deletions subsequently decrease the expression of Glycopeptidolipids (a significant surface lipid present in many non-tuberculous mycobacteria including Mycobacterium smegmatis ) and affect other physiological parameters like cell morphology, growth rate, biofilm formation, antibiotic susceptibility and existence within murine macrophages. Thus, unraveling the physiology of DD-CPases might help us design anti-mycobacterial therapeutics in future. Copyright © 2018 American Society for Microbiology.

Structural basis of death domain signaling in the p75 neurotrophin receptor

PubMed Central

Lin, Zhi; Tann, Jason Y; Goh, Eddy TH; Kelly, Claire; Lim, Kim Buay; Gao, Jian Fang; Ibanez, Carlos F

2015-01-01

Death domains (DDs) mediate assembly of oligomeric complexes for activation of downstream signaling pathways through incompletely understood mechanisms. Here we report structures of complexes formed by the DD of p75 neurotrophin receptor (p75NTR) with RhoGDI, for activation of the RhoA pathway, with caspase recruitment domain (CARD) of RIP2 kinase, for activation of the NF-kB pathway, and with itself, revealing how DD dimerization controls access of intracellular effectors to the receptor. RIP2 CARD and RhoGDI bind to p75NTR DD at partially overlapping epitopes with over 100-fold difference in affinity, revealing the mechanism by which RIP2 recruitment displaces RhoGDI upon ligand binding. The p75NTR DD forms non-covalent, low-affinity symmetric dimers in solution. The dimer interface overlaps with RIP2 CARD but not RhoGDI binding sites, supporting a model of receptor activation triggered by separation of DDs. These structures reveal how competitive protein-protein interactions orchestrate the hierarchical activation of downstream pathways in non-catalytic receptors. DOI: http://dx.doi.org/10.7554/eLife.11692.001 PMID:26646181

The Use of Adenovirus Dodecahedron in the Delivery of an Enzymatic Activity in the Cell

PubMed Central

Sumarheni; Gallet, Benoit; Fender, Pascal

2016-01-01

Penton-dodecahedron (Pt-Dd) derived from adenovirus type 3 is a symmetric complex of pentameric penton base plus fiber which can be produced in the baculovirus system at a high concentration. The size of Pt-Dd is smaller than the virus, but this virus-like particle (VLP) has the major proteins recognized by specific receptors on the surface of almost all types of cell. In this study, by direct observation with fluorescence microscopy on a fixed and living cell, the intracellular trafficking and localization of Pt-Dd labeled with fluorescence dyes in the cytoplasm of HeLa Tub-GFP showed a rapid internalization characteristic. Subsequently, the linkage of horseradish peroxidase (HRP) with Pt-Dd as the vector demonstrated an efficient system to deliver this enzyme into the cell without interfering its enzymatic activity as shown by biochemical and cellular experiments. These results were supported by additional studies using Bs-Dd or free form of the HRP used as the control. Overall, this study strengthens the potential role of Pt-Dd as an alternative vector for delivering therapeutic agents. PMID:27242929

Clinical predictors and gender-wise variations in dyssynergic defecation disorders.

PubMed

Jain, Mayank; Baijal, Rajiv; Srinivas, Melpakkam; Venkataraman, Jayanthi

2018-06-12

There is insufficient data from India regarding clinical predictors of dyssynergic defecation. To identify demography, symptom, and colonoscopic parameters that can predict dyssynergic defecation (DD) among patients with chronic constipation (CC) and to compare the profile among male and female patients with DD. Data collected from three centers during June 2014 to May 2017 included age, gender, symptom duration, form and consistency of stools, digital examination, colonoscopy, and anorectal manometry (ARM). Patients were grouped based on ARM diagnosis: group I (normal study) and group II (DD). The two groups were compared for demography, symptom profile, and colonoscopy findings. Gender-wise subset analysis was done for those with the normal and abnormal ARM using chi-square and unpaired t tests. Of 236 patients with CC evaluated, 130 (55%) had normal ARM and 106 (45%) had DD. Male sex, straining during defecation, bleeding per rectum, and abnormal colonoscopic diagnosis were significantly more common in group II. While bleeding per rectum and absence of urge to defecate were more common in males (p < 0.02), straining, digital evacuation, and hard stools were commoner in females with DD. Straining during defecation, bleeding per rectum, and abnormal colonoscopy findings were more common in patients with DD. Symptoms of bleeding per rectum and absence of urge to defecate in men and straining during defecation in female patients were significantly associated with DD. Symptoms differ in males and females with DD.

Army Hearing Program Talking Points Calendar Year 2015

DTIC Science & Technology

2016-12-14

outside the range of normal hearing sensitivity (greater than 25 dB), CY15 data.  Data: DOEHRS-HC Data Repository , Soldiers who had a DD2215 or...1.  Data: Defense Occupational and Environmental Health Readiness System-Hearing Conservation (DOEHRS-HC) Data Repository , CY15—Army Profile...Soldiers have a hearing loss that required a fit-for-duty (Readiness) evaluation:  An H-3 Hearing Profile.  Data: DOEHRS-HC Data Repository

Inhibition of DD-Peptidases by a Specific Trifluoroketone: Crystal Structure of a Complex with the Actinomadura R39 DD-Peptidase†

PubMed Central

Dzhekieva, Liudmila; Adediran, S. A.; Herman, Raphael; Kerff, Frédéric; Duez, Colette; Charlier, Paulette; Sauvage, Eric; Pratt, R.F.

2013-01-01

Inhibitors of bacterial DD-peptidases represent potential antibiotics. In the search for alternatives to β-lactams, we have investigated a series of compounds designed to generate transition state analogue structures on reaction with DD-peptidases. The compounds contain a combination of a peptidoglycan-mimetic specificity handle and a warhead capable of delivering a tetrahedral anion to the enzyme active site. The latter include a boronic acid, two alcohols, an aldehyde and a trifluoroketone. The compounds were tested against two low molecular mass class C DD-peptidases. As expected from previous observations, the boronic acid was a potent inhibitor, but, rather unexpectedly from precedent, the trifluoroketone [D-α-aminopimelyl-(1,1,1-trifluoro-3-amino)butan-2-one] was also very effective. Taking into account competing hydration, the trifluoroketone was the strongest inhibitor of the Actinomadura R39 DD-peptidase, with a subnanomolar (free ketone) inhibition constant. A crystal structure of the complex between the trifluoroketone and the R39 enzyme showed that a tetrahedral adduct had indeed formed with the active site serine nucleophile. The trifluoroketone moiety, therefore, should be considered along with boronic acids and phosphonates, as a warhead that can be incorporated into new and effective DD-peptidase inhibitors and therefore, perhaps, antibiotics. PMID:23484909

Inhibition of DD-peptidases by a specific trifluoroketone: crystal structure of a complex with the Actinomadura R39 DD-peptidase.

PubMed

Dzhekieva, Liudmila; Adediran, S A; Herman, Raphael; Kerff, Frédéric; Duez, Colette; Charlier, Paulette; Sauvage, Eric; Pratt, R F

2013-03-26

Inhibitors of bacterial DD-peptidases represent potential antibiotics. In the search for alternatives to β-lactams, we have investigated a series of compounds designed to generate transition state analogue structures upon reaction with DD-peptidases. The compounds contain a combination of a peptidoglycan-mimetic specificity handle and a warhead capable of delivering a tetrahedral anion to the enzyme active site. The latter includes a boronic acid, two alcohols, an aldehyde, and a trifluoroketone. The compounds were tested against two low-molecular mass class C DD-peptidases. As expected from previous observations, the boronic acid was a potent inhibitor, but rather unexpectedly from precedent, the trifluoroketone [D-α-aminopimelyl(1,1,1-trifluoro-3-amino)butan-2-one] was also very effective. Taking into account competing hydration, we found the trifluoroketone was the strongest inhibitor of the Actinomadura R39 DD-peptidase, with a subnanomolar (free ketone) inhibition constant. A crystal structure of the complex between the trifluoroketone and the R39 enzyme showed that a tetrahedral adduct had indeed formed with the active site serine nucleophile. The trifluoroketone moiety, therefore, should be considered along with boronic acids and phosphonates as a warhead that can be incorporated into new and effective DD-peptidase inhibitors and therefore, perhaps, antibiotics.

Mechanisms associated with the high adsorption of dibenzo-p-dioxin from water by smectite clays.

PubMed

Liu, Cun; Li, Hui; Teppen, Brian J; Johnston, Cliff T; Boyd, Stephen A

2009-04-15

Clay minerals may be an important unrecognized sorptive phase for dioxins in soils and clay deposits. Smectites, especially Cs-saponite, effectively adsorbed dibenzo-p-dioxin (DD) from water, reaching 0.8% (wt/wt). Adsorption was promoted by exchangeable cations with low hydration energies, and negative charge in the smectite arising from the tetrahedral siloxane sheets. X-ray diffraction measurements revealed that as DD loading increased to > or =8000 mg/kg the clay basal spacing increased abruptly from 12.3 to 15.2 A demonstrating DD intercalation. The 12.3 A spacing provides an interlayer distance that closely matches the molecular thickness of DD. In this configuration DD is essentially dehydrated as it interacts with the opposing hydrophobic siloxane sheets and with coplanar Cs+ via one of the dioxin ring oxygens. Ab initio calculations suggest that geometrical structures form at higher loadings in which intercalated DD molecules adopt a butterfly geometry sandwiched between dehydrated interlayer Cs+ and the siloxane surface, consistent with the 15.2 A spacing, wherein Cs+ interacts with dioxin ring oxygens and benzene ring pi-electrons. Fourier transformation infrared measurements confirm that adsorbed DD is present in orientations that are not parallel with the interlayer planar siloxane surfaces of smectite.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wang, Liwei; Yang, Jin Kuk; Kabaleeswaran, Venkataraman

The death-inducing signaling complex (DISC) formed by the death receptor Fas, the adaptor protein FADD and caspase-8 mediates the extrinsic apoptotic program. Mutations in Fas that disrupt the DISC cause autoimmune lymphoproliferative syndrome (ALPS). Here we show that the Fas-FADD death domain (DD) complex forms an asymmetric oligomeric structure composed of 5-7 Fas DD and 5 FADD DD, whose interfaces harbor ALPS-associated mutations. Structure-based mutations disrupt the Fas-FADD interaction in vitro and in living cells; the severity of a mutation correlates with the number of occurrences of a particular interaction in the structure. The highly oligomeric structure explains the requirementmore » for hexameric or membrane-bound FasL in Fas signaling. It also predicts strong dominant negative effects from Fas mutations, which are confirmed by signaling assays. The structure optimally positions the FADD death effector domain (DED) to interact with the caspase-8 DED for caspase recruitment and higher-order aggregation.« less

Synchronization of IM and HC: The Navy Perspective

DTIC Science & Technology

2010-07-01

grounds for automatic rejection of that weapon system for service use”. In 2003 , MIL-STD-2105C superseded MIL-STD-2105B by referencing the...34 Sandia National Laboratories, January 1987. [11] Tomasello , K., Sharp, M., Adams, J. and Rich Bowen. “Origin of Test Requirements and Passing...stimuli.” Department of Defense Policy • DoDD 5000.01, May 12, 2003 : E1.1.23. Safety. “… All systems containing energetics shall comply with insensitive

MQ-9 Reaper Unmanned Aircraft System (MQ-9 Reaper)

DTIC Science & Technology

2015-12-01

Selected Acquisition Report ( SAR ) RCS: DD-A&T(Q&A)823-424 MQ-9 Reaper Unmanned Aircraft System (MQ-9 Reaper) As of FY 2017 President’s Budget...Defense Acquisition Management Information Retrieval (DAMIR) March 23, 2016 16:18:05 UNCLASSIFIED MQ-9 Reaper December 2015 SAR March 23, 2016 16:18:05...2015 SAR March 23, 2016 16:18:05 UNCLASSIFIED 3 PB - President’s Budget PE - Program Element PEO - Program Executive Officer PM - Program Manager

AIM-120 Advanced Medium Range Air-to-Air Missile (AMRAAM)

DTIC Science & Technology

2015-12-01

Selected Acquisition Report ( SAR ) RCS: DD-A&T(Q&A)823-185 AIM-120 Advanced Medium Range Air-to-Air Missile (AMRAAM) As of FY 2017 President’s...Budget Defense Acquisition Management Information Retrieval (DAMIR) March 23, 2016 16:04:24 UNCLASSIFIED AMRAAM December 2015 SAR March 23, 2016 16:04...2015 SAR March 23, 2016 16:04:24 UNCLASSIFIED 3 PB - President’s Budget PE - Program Element PEO - Program Executive Officer PM - Program Manager POE

HC/MC-130 Recapitalization Aircraft (HC/MC-130 Recap)

DTIC Science & Technology

2015-12-01

Selected Acquisition Report ( SAR ) RCS: DD-A&T(Q&A)823-257 HC/MC-130 Recapitalization Aircraft (HC/MC-130 Recap) As of FY 2017 President’s Budget...Defense Acquisition Management Information Retrieval (DAMIR) March 18, 2016 08:18:54 UNCLASSIFIED HC/MC-130 Recap December 2015 SAR March 18, 2016...Recap December 2015 SAR March 18, 2016 08:18:54 UNCLASSIFIED 3 PB - President’s Budget PE - Program Element PEO - Program Executive Officer PM - Program

Integrating the Department of Defense Military Services’ Technology Development Programs to Improve Time, Cost, and Technical Quality Parameters

DTIC Science & Technology

2007-03-01

the DoD in general and across the SR, DD(X), and FCS programs in particular. The findings of this study show that through careful planning and...FCS programs in particular. The findings of this study show that through careful planning and coordinated technology transition, DoD acquisition...careful planning and coordinated technology transition, DoD acquisition programs can indeed leverage the technology development efforts of the three

Department of Defense Joint Technical Architecture, Version 6.0. Volume 2

DTIC Science & Technology

2003-10-03

information needs of our warfighters and the DoD enterprise. Information assurance will be integral to the GIG, and data management strategy initiatives will...IT investment strategy , and integration JTA Version 6.0, Final 3 October 2003 Vol. II–iv Executive Summaryoversight. DoDD 8100.1 establishes the GIG as... Strategy (May 9, 2003). In addition, numerous standards have been marked sunset, indicating deletion from the JTA on a future date to be determined by a

CH-53K Heavy Lift Replacement Helicopter (CH-53K)

DTIC Science & Technology

2015-12-01

Selected Acquisition Report (SAR) RCS: DD-A&T(Q&A)823-390 CH-53K Heavy Lift Replacement Helicopter (CH-53K) As of FY 2017 President’s Budget...December 2015 SAR March 4, 2016 10:04:18 UNCLASSIFIED 4 Col Henry Vanderborght PMA-261 Heavy Lift Helicopters Program Executive Office - Air, Anti...Replacement Helicopter (CH-53K) DoD Component Navy Responsible Office References SAR Baseline (Development Estimate) Defense Acquisition Executive (DAE

Flexible Stoichiometry and Asymmetry of the PIDDosome Core Complex by Heteronuclear NMR Spectroscopy and Mass Spectrometry

PubMed Central

Nematollahi, Lily A.; Garza-Garcia, Acely; Bechara, Chérine; Esposito, Diego; Morgner, Nina; Robinson, Carol V.; Driscoll, Paul C.

2015-01-01

Homotypic death domain (DD)–DD interactions are important in the assembly of oligomeric signaling complexes such as the PIDDosome that acts as a platform for activation of caspase-2-dependent apoptotic signaling. The structure of the PIDDosome core complex exhibits an asymmetric three-layered arrangement containing five PIDD-DDs in one layer, five RAIDD-DDs in a second layer and an additional two RAIDD-DDs. We addressed complex formation between PIDD-DD and RAIDD-DD in solution using heteronuclear nuclear magnetic resonance (NMR) spectroscopy, nanoflow electrospray ionization mass spectrometry and size-exclusion chromatography with multi-angle light scattering. The DDs assemble into complexes displaying molecular masses in the range 130–158 kDa and RAIDD-DD:PIDD-DD stoichiometries of 5:5, 6:5 and 7:5. These data suggest that the crystal structure is representative of only the heaviest species in solution and that two RAIDD-DDs are loosely attached to the 5:5 core. Two-dimensional 1H,15N-NMR experiments exhibited signal loss upon complexation consistent with the formation of high-molecular-weight species. 13C-Methyl-transverse relaxation optimized spectroscopy measurements of the PIDDosome core exhibit signs of differential line broadening, cross-peak splitting and chemical shift heterogeneity that reflect the presence of non-equivalent sites at interfaces within an asymmetric complex. Experiments using a mutant RAIDD-DD that forms a monodisperse 5:5 complex with PIDD-DD show that the spectroscopic signature derives from the quasi- but non-exact equivalent environments of each DD. Since this characteristic was previously demonstrated for the complex between the DDs of CD95 and FADD, the NMR data for this system are consistent with the formation of a structure homologous to the PIDDosome core. PMID:25528640

The impact of Dupuytren disease on patient activity and quality of life.

PubMed

Wilburn, J; McKenna, S P; Perry-Hinsley, D; Bayat, A

2013-06-01

To explore the impact of Dupuytren disease (DD) from the patients' perspective. Audio-recorded interviews were conducted for patients with Dupuytren disease (DD) attending outpatient clinics. The interviews were transcribed and subjected to content analysis. This analysis highlighted key impact areas and common themes in individuals' personal experiences. These were then allocated to categories specified by the World Health Organization International Classification of Functioning, Disability, and Health (impairments and activity limitations) and the needs-based model of quality of life (QoL). Qualitative unstructured interviews were conducted with 34 patients (74% men; age, 41-80 y; mean [SD], 64 [13] y). The sample had a wide range of severity and duration of DD (range, 0.5-40; mean [SD], 13 [10] y). Nine hundred fifty-three statements relating to the impact of DD were identified from the interview transcripts. These statements fell into 2 major categories of impact: activity limitations (10 themes including problems with dressing, gripping, and personal care) and QoL (6 need categories: physiological, safety and security, social, affection, esteem, and cognitive needs). Findings from the interviews suggest that DD affects both performance of activities and QoL. To determine accurately the effectiveness of DD interventions from the patients' perspective, it is important to determine their impacts on both activity limitations and QoL. We intend to develop valid, reproducible, and responsive DD-specific scales for this purpose. The study identifies key issues specific to DD that influence patients' functioning and QoL. The information reported will form the basis of DD-specific patient-reported outcomes measures for use in clinical practice and evaluations of interventions. Copyright © 2013 American Society for Surgery of the Hand. Published by Elsevier Inc. All rights reserved.

Psychometric validation of the dysmenorrhea daily diary (DysDD): a patient-reported outcome for dysmenorrhea.

PubMed

Nguyen, Allison M; Arbuckle, Rob; Korver, Tjeerd; Chen, Fang; Taylor, Beverley; Turnbull, Alice; Norquist, Josephine M

2017-08-01

The objective of this study was to evaluate the psychometric properties of the Dysmenorrhea Daily Diary (DysDD), an electronic patient-reported outcome, in a sample of 355 women with primary dysmenorrhea enrolled in a phase IIb, multicenter, randomized, partially blinded, placebo-controlled trial for treatment of dysmenorrhea. Subjects completed the DysDD over three menstrual cycles, one pre-treatment baseline cycle and two treatment cycles. The DysDD was administered alongside the Menstrual Distress Questionnaire (MDQ), the Short-Form 36 Version 2.0 (SF-36v2), and a Global Assessment of Change (GAC). Item response distributions, test-retest reliability, concurrent and known groups validity, responsiveness, and minimally important difference (MID) were evaluated for the DysDD. As expected, item response distributions varied throughout the menstrual period for all items, with the response scales fully utilized. Within-cycle test-retest reliability was adequate (weighted kappa: 0.5-0.7), although between-cycle test-retest was poor (weighted kappa: 0.1-0.5), most likely due to the highly variable nature of dysmenorrhea between cycles rather than limitations of the measure. Correlations with the MDQ and SF-36v2 were low-moderate, but in the predicted direction, supporting concurrent validity. There were significant differences in DysDD scores across severity groups based on pain medication use. The DysDD was responsive to changes in patients' dysmenorrhea with significantly different changes in scores between change groups (p < 0.0001). MID analyses suggest changes on the DysDD 0-10 pelvic pain score of three points can be considered clinically meaningful. Overall, findings indicate that the DysDD has acceptable reliability and is a valid and responsive instrument for assessing dysmenorrhea.

Structural and Functional Characterization of the Recombinant Death Domain from Death-Associated Protein Kinase

PubMed Central

Dioletis, Evangelos; Dingley, Andrew J.; Driscoll, Paul C.

2013-01-01

Death-associated protein kinase (DAPk) is a calcium/calmodulin-regulated Ser/Thr-protein kinase that functions at an important point of integration for cell death signaling pathways. DAPk has a structurally unique multi-domain architecture, including a C-terminally positioned death domain (DD) that is a positive regulator of DAPk activity. In this study, recombinant DAPk-DD was observed to aggregate readily and could not be prepared in sufficient yield for structural analysis. However, DAPk-DD could be obtained as a soluble protein in the form of a translational fusion protein with the B1 domain of streptococcal protein G. In contrast to other DDs that adopt the canonical six amphipathic α-helices arranged in a compact fold, the DAPk-DD was found to possess surprisingly low regular secondary structure content and an absence of a stable globular fold, as determined by circular dichroism (CD), NMR spectroscopy and a temperature-dependent fluorescence assay. Furthermore, we measured the in vitro interaction between extracellular-regulated kinase-2 (ERK2) and various recombinant DAPk-DD constructs. Despite the low level of structural order, the recombinant DAPk-DD retained the ability to interact with ERK2 in a 1∶1 ratio with a K d in the low micromolar range. Only the full-length DAPk-DD could bind ERK2, indicating that the apparent ‘D-motif’ located in the putative sixth helix of DAPk-DD is not sufficient for ERK2 recognition. CD analysis revealed that binding of DAPk-DD to ERK2 is not accompanied by a significant change in secondary structure. Taken together our data argue that the DAPk-DD, when expressed in isolation, does not adopt a classical DD fold, yet in this state retains the capacity to interact with at least one of its binding partners. The lack of a stable globular structure for the DAPk-DD may reflect either that its folding would be supported by interactions absent in our experimental set-up, or a limitation in the structural bioinformatics assignment of the three-dimensional structure. PMID:23922916

Structural and functional characterization of the recombinant death domain from death-associated protein kinase.

PubMed

Dioletis, Evangelos; Dingley, Andrew J; Driscoll, Paul C

2013-01-01

Death-associated protein kinase (DAPk) is a calcium/calmodulin-regulated Ser/Thr-protein kinase that functions at an important point of integration for cell death signaling pathways. DAPk has a structurally unique multi-domain architecture, including a C-terminally positioned death domain (DD) that is a positive regulator of DAPk activity. In this study, recombinant DAPk-DD was observed to aggregate readily and could not be prepared in sufficient yield for structural analysis. However, DAPk-DD could be obtained as a soluble protein in the form of a translational fusion protein with the B1 domain of streptococcal protein G. In contrast to other DDs that adopt the canonical six amphipathic α-helices arranged in a compact fold, the DAPk-DD was found to possess surprisingly low regular secondary structure content and an absence of a stable globular fold, as determined by circular dichroism (CD), NMR spectroscopy and a temperature-dependent fluorescence assay. Furthermore, we measured the in vitro interaction between extracellular-regulated kinase-2 (ERK2) and various recombinant DAPk-DD constructs. Despite the low level of structural order, the recombinant DAPk-DD retained the ability to interact with ERK2 in a 1∶1 ratio with a K d in the low micromolar range. Only the full-length DAPk-DD could bind ERK2, indicating that the apparent 'D-motif' located in the putative sixth helix of DAPk-DD is not sufficient for ERK2 recognition. CD analysis revealed that binding of DAPk-DD to ERK2 is not accompanied by a significant change in secondary structure. Taken together our data argue that the DAPk-DD, when expressed in isolation, does not adopt a classical DD fold, yet in this state retains the capacity to interact with at least one of its binding partners. The lack of a stable globular structure for the DAPk-DD may reflect either that its folding would be supported by interactions absent in our experimental set-up, or a limitation in the structural bioinformatics assignment of the three-dimensional structure.

A new double digestion ligation mediated suppression PCR method for simultaneous bacteria DNA-typing and confirmation of species: an Acinetobacter sp. model.

PubMed

Stojowska, Karolina; Krawczyk, Beata

2014-01-01

We have designed a new ddLMS PCR (double digestion Ligation Mediated Suppression PCR) method based on restriction site polymorphism upstream from the specific target sequence for the simultaneous identification and differentiation of bacterial strains. The ddLMS PCR combines a simple PCR used for species or genus identification and the LM PCR strategy for strain differentiation. The bacterial identification is confirmed in the form of the PCR product(s), while the length of the PCR product makes it possible to differentiate between bacterial strains. If there is a single copy of the target sequence within genomic DNA, one specific PCR product is created (simplex ddLMS PCR), whereas for multiple copies of the gene the fingerprinting patterns can be obtained (multiplex ddLMS PCR). The described ddLMS PCR method is designed for rapid and specific strain differentiation in medical and microbiological studies. In comparison to other LM PCR it has substantial advantages: enables specific species' DNA-typing without the need for pure bacterial culture selection, is not sensitive to contamination with other cells or genomic DNA, and gives univocal "band-based" results, which are easy to interpret. The utility of ddLMS PCR was shown for Acinetobacter calcoaceticus-baumannii (Acb) complex, the genetically closely related and phenotypically similar species and also important nosocomial pathogens, for which currently, there are no recommended methods for screening, typing and identification. In this article two models are proposed: 3' recA-ddLMS PCR-MaeII/RsaI for Acb complex interspecific typing and 5' rrn-ddLMS PCR-HindIII/ApaI for Acinetobacter baumannii intraspecific typing. ddLMS PCR allows not only for DNA-typing but also for confirmation of species in one reaction. Also, practical guidelines for designing a diagnostic test based on ddLMS PCR for genotyping different species of bacteria are provided.

A New Double Digestion Ligation Mediated Suppression PCR Method for Simultaneous Bacteria DNA-Typing and Confirmation of Species: An Acinetobacter sp. Model

PubMed Central

Stojowska, Karolina; Krawczyk, Beata

2014-01-01

We have designed a new ddLMS PCR (double digestion Ligation Mediated Suppression PCR) method based on restriction site polymorphism upstream from the specific target sequence for the simultaneous identification and differentiation of bacterial strains. The ddLMS PCR combines a simple PCR used for species or genus identification and the LM PCR strategy for strain differentiation. The bacterial identification is confirmed in the form of the PCR product(s), while the length of the PCR product makes it possible to differentiate between bacterial strains. If there is a single copy of the target sequence within genomic DNA, one specific PCR product is created (simplex ddLMS PCR), whereas for multiple copies of the gene the fingerprinting patterns can be obtained (multiplex ddLMS PCR). The described ddLMS PCR method is designed for rapid and specific strain differentiation in medical and microbiological studies. In comparison to other LM PCR it has substantial advantages: enables specific species' DNA-typing without the need for pure bacterial culture selection, is not sensitive to contamination with other cells or genomic DNA, and gives univocal “band-based” results, which are easy to interpret. The utility of ddLMS PCR was shown for Acinetobacter calcoaceticus-baumannii (Acb) complex, the genetically closely related and phenotypically similar species and also important nosocomial pathogens, for which currently, there are no recommended methods for screening, typing and identification. In this article two models are proposed: 3′ recA-ddLMS PCR-MaeII/RsaI for Acb complex interspecific typing and 5′ rrn-ddLMS PCR-HindIII/ApaI for Acinetobacter baumannii intraspecific typing. ddLMS PCR allows not only for DNA-typing but also for confirmation of species in one reaction. Also, practical guidelines for designing a diagnostic test based on ddLMS PCR for genotyping different species of bacteria are provided. PMID:25522278

Neutron Yield With a Pulsed Surface Flashover Deuterium Source

NASA Astrophysics Data System (ADS)

Guethlein, G.; Falabella, S.; Sampayan, S. E.; Meyer, G.; Tang, V.; Kerr, P.

2009-03-01

As a step towards developing an ultra compact D-D neutron source for various defense and homeland security applications, a compact, low average power ion source is needed. Towards that end, we are testing a high current, pulsed surface flashover ion source, with deuterated titanium as the spark contacts. Neutron yield and source lifetime data will be presented using a low voltage (<100 kV) deuterated target. With 20 ns spark drive pulses we have shown >106 neutrons/s with 1 kHz PRF

Operation SANDSTONE: 1948

DTIC Science & Technology

1983-12-19

8217qOKINWOTME X_ ENJEBI LR UNU/ MIJ!KADREK P, 1BOKOLU BO) KENE LAB .-. •ELLE ,AEJ • LUJORS• I i F L E 149N" YOKE (49 KT). TOWVER I,. L QJWA% ALIE’.I t...MSRB-60 12 Cy ATTN: DD Merchant Marine Academy Field Comand ATTN: Director of Libraries Defense iuclear Agency Naval Historical Center ATTN: FCLS, MAJ...Judge Adv Gen ATTN: OMA, DP-22 ATTN: Code 73 Nevada Operations Office U.S. Merchant Marine Academy ATTN: Health Physics Div ATTN: Librarian 2 cy ATTN: R

MQ-8 Fire Scout Unmanned Aircraft System (MQ-8 Fire Scout)

DTIC Science & Technology

2015-12-01

Selected Acquisition Report ( SAR ) RCS: DD-A&T(Q&A)823-253 MQ-8 Fire Scout Unmanned Aircraft System (MQ-8 Fire Scout) As of FY 2017 President’s...Budget Defense Acquisition Management Information Retrieval (DAMIR) March 8, 2016 11:20:32 UNCLASSIFIED MQ-8 Fire Scout December 2015 SAR March 8, 2016...Scout December 2015 SAR March 8, 2016 11:20:32 UNCLASSIFIED 3 PB - President’s Budget PE - Program Element PEO - Program Executive Officer PM - Program

MQ-1C Gray Eagle Unmanned Aircraft System (MQ-1C Gray Eagle)

DTIC Science & Technology

2015-12-01

Selected Acquisition Report ( SAR ) RCS: DD-A&T(Q&A)823-420 MQ-1C Gray Eagle Unmanned Aircraft System (MQ-1C Gray Eagle) As of FY 2017 President’s...Budget Defense Acquisition Management Information Retrieval (DAMIR) March 21, 2016 17:33:19 UNCLASSIFIED MQ-1C Gray Eagle December 2015 SAR March 21...Gray Eagle December 2015 SAR March 21, 2016 17:33:19 UNCLASSIFIED 3 PB - President’s Budget PE - Program Element PEO - Program Executive Officer PM

LPD 17 San Antonio Class Amphibious Transport Dock (LPD 17)

DTIC Science & Technology

2015-12-01

Selected Acquisition Report ( SAR ) RCS: DD-A&T(Q&A)823-542 LPD 17 San Antonio Class Amphibious Transport Dock (LPD 17) As of FY 2017 President’s...Budget Defense Acquisition Management Information Retrieval (DAMIR) March 8, 2016 11:18:10 UNCLASSIFIED LPD 17 December 2015 SAR March 8, 2016 11:18:10... SAR March 8, 2016 11:18:10 UNCLASSIFIED 3 PB - President’s Budget PE - Program Element PEO - Program Executive Officer PM - Program Manager POE

Joint Precision Approach and Landing System Increment 1A (JPALS Inc 1A)

DTIC Science & Technology

2015-12-01

Selected Acquisition Report ( SAR ) RCS: DD-A&T(Q&A)823-238 Joint Precision Approach and Landing System Increment 1A (JPALS Inc 1A) As of FY 2017...President’s Budget Defense Acquisition Management Information Retrieval (DAMIR) March 10, 2016 11:30:56 UNCLASSIFIED JPALS Inc 1A December 2015 SAR ...Cost JPALS Inc 1A December 2015 SAR March 10, 2016 11:30:56 UNCLASSIFIED 3 PB - President’s Budget PE - Program Element PEO - Program Executive Officer

CVN 78 Gerald R. Ford Class Nuclear Aircraft Carrier (CVN 78)

DTIC Science & Technology

2015-12-01

Selected Acquisition Report ( SAR ) RCS: DD-A&T(Q&A)823-223 CVN 78 Gerald R. Ford Class Nuclear Aircraft Carrier (CVN 78) As of FY 2017 President’s...Budget Defense Acquisition Management Information Retrieval (DAMIR) March 21, 2016 17:17:44 UNCLASSIFIED CVN 78 December 2015 SAR March 21, 2016 17...December 2015 SAR March 21, 2016 17:17:44 UNCLASSIFIED 3 PB - President’s Budget PE - Program Element PEO - Program Executive Officer PM - Program Manager

Navy DD(X) and LCS Ship Acquisition Programs: Oversight Issues and Options for Congress

DTIC Science & Technology

2004-10-28

NUMBER 5e . TASK NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) Defense Acquisition University David D. Acker Library...program, it had decided to award the contract for the next phase on a sole-source basis to NGSS . The Navy has also stated that “The ship construction...contracts will be allocated equally between NGSS and BIW for the first six ships... and will be Cost Plus Incentive Fee (CPIF) type contracts.... The

Acquisition and Competition Strategy Options for the DD(X): The U.S. Navy’s 21st Century Destroyer

DTIC Science & Technology

2006-01-01

5e . TASK NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) Rand Corporation,National Defense Research Institute,1776...In April 2002, one of those teams, led by Nor- throp Grumman Ship Systems ( NGSS ), was selected winner of the competition and awarded a $2.9 billion...Northrop Grumman Ship Systems ( NGSS ) and including Raytheon Systems Co. was selected as winner of that competition and placed under contract to

Mobilization and Defense Management Technical Reports Series. Achievement of Industrial Mobilization Objectives in an Economically Interdependent World.

DTIC Science & Technology

1983-05-01

maintenance of the essential industrial base and to put in place the framework and processes---’ DD I O 1473 £O,TION OF I NOV65 IS OBSOLETE{ SECjBITY...the essential indu,, ......... -: -, :.It .n pl3ce the framework and processes necessary to resolve the conflict between liberal trade and national...domestic capabilities of selected industries essential to national security. Major Recommendations 1. Modify U.S. trade policy to allow for goverr ent

32 CFR 811.8 - Forms prescribed and availability of publications.

Code of Federal Regulations, 2010 CFR

2010-07-01

... FORCE SALES AND SERVICES RELEASE, DISSEMINATION, AND SALE OF VISUAL INFORMATION MATERIALS § 811.8 Forms prescribed and availability of publications. (a) AF Form 833, Visual Information Request, AF Form 1340, Visual Information Support Center Workload Report, DD Form 1995, Visual Information (VI) Production...

Differences in trauma history and psychopathology between PTSD patients with and without co-occurring dissociative disorders

PubMed Central

Wabnitz, Pascal; Gast, Ursula; Catani, Claudia

2013-01-01

Background The interplay between different types of potentially traumatizing events, posttraumatic symptoms, and the pathogenesis of PTSD or major dissociative disorders (DD) has been extensively studied during the last decade. However, the phenomenology and nosological classification of posttraumatic disorders is currently under debate. The current study was conducted to investigate differences between PTSD patients with and without co-occurring major DD with regard to general psychopathology, trauma history, and trauma-specific symptoms. Methods Twenty-four inpatients were administered the Clinician-Administered PTSD Scale for DSM-IV (CAPS) and the Mini-Structured Clinical Interview for DSM-IV Dissociative Disorders (MINI-SKID-D) to assess DD and PTSD. Additionally, participants completed questionnaires to assess general psychopathology and health status. Results Symptom profiles and axis I comorbidity were similar in all patients. Traumatic experiences did not differ between the two groups, with both reporting high levels of childhood trauma. Only trauma-specific avoidance behavior and dissociative symptoms differed between groups. Conclusion Results support the view that PTSD and DD are affiliated disorders that could be classified within the same diagnostic category. Our results accord with a typological model of dissociation in which profound forms of dissociation are specific to DD and are accompanied with higher levels of trauma-specific avoidance in DD patients. PMID:24298325

Differences in trauma history and psychopathology between PTSD patients with and without co-occurring dissociative disorders.

PubMed

Wabnitz, Pascal; Gast, Ursula; Catani, Claudia

2013-01-01

The interplay between different types of potentially traumatizing events, posttraumatic symptoms, and the pathogenesis of PTSD or major dissociative disorders (DD) has been extensively studied during the last decade. However, the phenomenology and nosological classification of posttraumatic disorders is currently under debate. The current study was conducted to investigate differences between PTSD patients with and without co-occurring major DD with regard to general psychopathology, trauma history, and trauma-specific symptoms. Twenty-four inpatients were administered the Clinician-Administered PTSD Scale for DSM-IV (CAPS) and the Mini-Structured Clinical Interview for DSM-IV Dissociative Disorders (MINI-SKID-D) to assess DD and PTSD. Additionally, participants completed questionnaires to assess general psychopathology and health status. Symptom profiles and axis I comorbidity were similar in all patients. Traumatic experiences did not differ between the two groups, with both reporting high levels of childhood trauma. Only trauma-specific avoidance behavior and dissociative symptoms differed between groups. Results support the view that PTSD and DD are affiliated disorders that could be classified within the same diagnostic category. Our results accord with a typological model of dissociation in which profound forms of dissociation are specific to DD and are accompanied with higher levels of trauma-specific avoidance in DD patients.

Identification of the Calmodulin-Binding Domains of Fas Death Receptor

PubMed Central

Chang, Bliss J.; Samal, Alexandra B.; Vlach, Jiri; Fernandez, Timothy F.; Brooke, Dewey; Prevelige, Peter E.; Saad, Jamil S.

2016-01-01

The extrinsic apoptotic pathway is initiated by binding of a Fas ligand to the ectodomain of the surface death receptor Fas protein. Subsequently, the intracellular death domain of Fas (FasDD) and that of the Fas-associated protein (FADD) interact to form the core of the death-inducing signaling complex (DISC), a crucial step for activation of caspases that induce cell death. Previous studies have shown that calmodulin (CaM) is recruited into the DISC in cholangiocarcinoma cells and specifically interacts with FasDD to regulate the apoptotic/survival signaling pathway. Inhibition of CaM activity in DISC stimulates apoptosis significantly. We have recently shown that CaM forms a ternary complex with FasDD (2:1 CaM:FasDD). However, the molecular mechanism by which CaM binds to two distinct FasDD motifs is not fully understood. Here, we employed mass spectrometry, nuclear magnetic resonance (NMR), biophysical, and biochemical methods to identify the binding regions of FasDD and provide a molecular basis for the role of CaM in Fas–mediated apoptosis. Proteolytic digestion and mass spectrometry data revealed that peptides spanning residues 209–239 (Fas-Pep1) and 251–288 (Fas-Pep2) constitute the two CaM-binding regions of FasDD. To determine the molecular mechanism of interaction, we have characterized the binding of recombinant/synthetic Fas-Pep1 and Fas-Pep2 peptides with CaM. Our data show that both peptides engage the N- and C-terminal lobes of CaM simultaneously. Binding of Fas-Pep1 to CaM is entropically driven while that of Fas-Pep2 to CaM is enthalpically driven, indicating that a combination of electrostatic and hydrophobic forces contribute to the stabilization of the FasDD–CaM complex. Our data suggest that because Fas-Pep1 and Fas-Pep2 are involved in extensive intermolecular contacts with the death domain of FADD, binding of CaM to these regions may hinder its ability to bind to FADD, thus greatly inhibiting the initiation of apoptotic signaling pathway. PMID:26735300

Analysis of a homologue of the adducin head gene which is a potential target for the Dictyostelium STAT protein Dd-STATa.

PubMed

Aoshima, Ryota; Hiraoka, Rieko; Shimada, Nao; Kawata, Takefumi

2006-01-01

A Dd-STATa-null mutant, which is defective in expression of a Dictyostelium homologue of the metazoan STAT (signal transducers and activators of transcription) proteins, fails to culminate and this phenotype correlates with the loss of expression of various prestalk (pst) genes. An EST clone, SSK395, encodes a close homologue of the adducin amino-terminal head domain and harbors a putative actin-binding domain. We fused promoter fragments of the cognate gene, ahhA (adducin head homologue A), to a lacZ reporter and determined their expression pattern. The proximal promoter region is necessary for the expression of ahhA at an early (pre-aggregative) stage of development and this expression is Dd-STATa independent. The distal promoter region is necessary for expression at later stages of development in pstA cells, of the slug and in upper cup and pstAB cells during culmination. The distal region is partly Dd-STATa-dependent. The ahhA-null mutant develops almost normally until culmination, but it forms slanting culminants that tend to collapse on to the substratum. The mutant also occasionally forms fruiting bodies with swollen papillae and with constrictions in the prestalk region. The AhhA protein localizes to the stalk tube entrance and also to the upper cup cells and in cells at or near to the constricted region where an F-actin ring is localized. These findings suggest that Dd-STATa regulates culmination and may be necessary for straight downward elongation of the stalk, via the putative actin-binding protein AhhA.

Three-body DD{pi} dynamics for the X(3872)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Baru, V.; Filin, A. A.; Hanhart, C.

2011-10-01

We investigate the role played by the three-body DD{pi} dynamics on the near-threshold resonance X(3872) charmonium state, which is assumed to be formed by nonperturbative DD{sup *} dynamics. It is demonstrated that, as compared to the naive static-pions approximation, the imaginary parts that originate from the inclusion of dynamical pions reduce substantially the width from the DD{pi} intermediate state. In particular, for a resonance peaked at 0.5 MeV below the D{sup 0}D{sup *0} threshold, this contribution to the width is reduced by about a factor of 2, and the effect of the pion dynamics on the width grows as longmore » as the resonance is shifted towards the D{sup 0}D{sup 0{pi}0} threshold. Although the physical width of the X is dominated by inelastic channels, our finding should still be of importance for the X line shapes in the DD{pi} channel below DD{sup *} threshold. For example, in the scattering length approximation, the imaginary part of the scattering length includes effects of all the pion dynamics and does not only stem from the D{sup *} width. Meanwhile, we find that another important quantity for the X phenomenology, the residue at the X pole, is weakly sensitive to dynamical pions. In particular, we find that the binding energy dependence of this quantity from the full calculation is close to that found from a model with pointlike DD{sup *} interactions only, consistent with earlier claims. Coupled-channel effects (inclusion of the charged DD{sup *} channel) turn out to have a moderate impact on the results.« less

Accurate quantitation of circulating cell-free mitochondrial DNA in plasma by droplet digital PCR.

PubMed

Ye, Wei; Tang, Xiaojun; Liu, Chu; Wen, Chaowei; Li, Wei; Lyu, Jianxin

2017-04-01

To establish a method for accurate quantitation of circulating cell-free mitochondrial DNA (ccf-mtDNA) in plasma by droplet digital PCR (ddPCR), we designed a ddPCR method to determine the copy number of ccf-mtDNA by amplifying mitochondrial ND1 (MT-ND1). To evaluate the sensitivity and specificity of the method, a recombinant pMD18-T plasmid containing MT-ND1 sequences and mtDNA-deleted (ρ 0 ) HeLa cells were used, respectively. Subsequently, different plasma samples were prepared for ddPCR to evaluate the feasibility of detecting plasma ccf-mtDNA. In the results, the ddPCR method showed high sensitivity and specificity. When the DNA was extracted from plasma prior to ddPCR, the ccf-mtDNA copy number was higher than that measured without extraction. This difference was not due to a PCR inhibitor, such as EDTA-Na 2 , an anti-coagulant in plasma, because standard EDTA-Na 2 concentration (5 mM) did not significantly inhibit ddPCR reactions. The difference might be attributable to plasma exosomal mtDNA, which was 4.21 ± 0.38 copies/μL of plasma, accounting for ∼19% of plasma ccf-mtDNA. Therefore, ddPCR can quickly and reliably detect ccf-mtDNA from plasma with a prior DNA extraction step, providing for a more accurate detection of ccf-mtDNA. The direct use of plasma as a template in ddPCR is suitable for the detection of exogenous cell-free nucleic acids within plasma, but not of nucleic acids that have a vesicle-associated form, such as exosomal mtDNA. Graphical Abstract Designs of the present work. *: Module 1, #: Module 2, &: Module 3.

Simultaneous Quantification of Multiple Alternatively Spliced mRNA Transcripts Using Droplet Digital PCR.

PubMed

Sun, Bing; Zheng, Yun-Ling

2018-01-01

Currently there is no sensitive, precise, and reproducible method to quantitate alternative splicing of mRNA transcripts. Droplet digital™ PCR (ddPCR™) analysis allows for accurate digital counting for quantification of gene expression. Human telomerase reverse transcriptase (hTERT) is one of the essential components required for telomerase activity and for the maintenance of telomeres. Several alternatively spliced forms of hTERT mRNA in human primary and tumor cells have been reported in the literature. Using one pair of primers and two probes for hTERT, four alternatively spliced forms of hTERT (α-/β+, α+/β- single deletions, α-/β- double deletion, and nondeletion α+/β+) were accurately quantified through a novel analysis method via data collected from a single ddPCR reaction. In this chapter, we describe this ddPCR method that enables direct quantitative comparison of four alternatively spliced forms of the hTERT messenger RNA without the need for internal standards or multiple pairs of primers specific for each variant, eliminating the technical variation due to differential PCR amplification efficiency for different amplicons and the challenges of quantification using standard curves. This simple and straightforward method should have general utility for quantifying alternatively spliced gene transcripts.

Major Management Challenges and Program Risks. Department of State

DTIC Science & Technology

2001-01-01

Major Management Challenges and Program Risks Department of StateGAO-01-252 Form SF298 Citation Data Report Date ("DD MON YYYY") 00JAN2001 Report...Type N/A Dates Covered (from... to) ("DD MON YYYY") Title and Subtitle Major Management Challenges and Program Risks Department of State Contract or...special series, first issued in January 1999, entitled the Performance and Accountability Series: Major Management Challenges and Program Risks . In

Visual abnormalities associated with enhanced optic nerve myelination.

PubMed

Yu, Minzhong; Narayanan, S Priyadarshini; Wang, Feng; Morse, Emily; Macklin, Wendy B; Peachey, Neal S

2011-02-16

Expression of the constitutively active serine/threonine kinase Akt in oligodendrocytes results in enhanced myelination in the CNS. Here, we have examined the effects of this Akt overexpression on optic nerve structure and on optic nerve function, assessed using the visual evoked potential (VEP). Transgenic mice have been generated with the Plp promoter driving expression of a modified form of Akt, in which aspartic acids are substituted for Thr308 and Ser473. These Plp-Akt-DD (Akt-DD) mice, and littermate controls, were studied at different ages. Optic nerves were examined anatomically at 2 and 6 months of age. At 2 months of age, optic nerves were substantially thicker in Akt-DD mice, reflecting an increase in myelination of optic nerve axons. By electron microscopy, myelin thickness was increased in Akt-DD optic nerve, with extended paranodal domains having excess paranodal loops, and the density of nodes of Ranvier was reduced, relative to control mice. We recorded VEPs in response to strobe flash ganzfeld stimuli presented after overnight dark- and light-adapted conditions at ages ranging from 1 to 10 months. It was possible to record a clear VEP from Akt-DD mice at all ages examined. At 1 month of age, VEP implicit times were somewhat shorter in Akt-DD transgenic mice than in control animals. Beyond 6months of age, VEP latencies were consistently delayed in Akt-DD transgenic mice. These abnormalities did not reflect an alteration in retinal function as there were no significant differences between ERGs obtained from control or Akt-DD transgenic mice. In young mice, the somewhat faster responses may reflect improved transmission due to increased myelination of optic nerve axons. In older mice, where the Akt-DD optic nerve is markedly thicker than control, it is remarkable that optic nerves continue to function. Copyright © 2010 Elsevier B.V. All rights reserved.

Assessment of Quality of Life (QOL) in Obsessive Compulsive Disorder (OCD) and Dysthymic Disorder (DD): A Comparative Study.

PubMed

Vasudev, Roopesh Gopal Nariyandada; Yallappa, Sudarshan Chikkanayakanahalli; Saya, Ganesh Kumar

2015-05-01

There is paucity of data on Quality of Life (QOL) in non-psychotic mental disorders. To assess the Quality of Life (QOL) in Obsessive Compulsive Disorder (OCD) and compare it with Dysthymic Disorder (DD). This hospital based cross-sectional study was conducted in 30 patients each diagnosed to have OCD and DD during October 2009 to September 2011 in a medical institution in Davanagere, Karnataka, India. DSM-IV-TR (Diagnostic statistical manual-IV-Text Revised) criteria was used for diagnosis of OCD and DD. Yale Brown Obsessive Compulsive Scale (Y-BOCS) and WHO QOL BREF (brief form) scales were used. Data was analysed by independent sample t-test. Overall QOL score was 51.07(SD=11.47) and 50.91(SD=7.41) in OCD and DD groups respectively. QOL score was comparatively low in psychological domain with score of 44.12(SD=14.14) and 45.10(12.35) in OCD and DD respectively. There was no significant difference in the QOL score of the two groups with respect to socio demographic variables in OCD group, but it was different with respect to place of residence in DD group (p<0.05). In Q1(perception on quality of life) and Q2 (perception on quality of health) domain, QOL score was marginally higher than average in both the groups. In Y-BOCS scale, no statistical significant association was found between severity of OCD and QOL score in each of the domains (p>0.05). Overall QOL score was average and there was no significant difference of QOL score between the OCD and DD groups implying that both these non-psychotic mental disorders may have same influence or effect on QOL of the subjects. Further analytical studies will explore the associated factors of QOL in OCD and DD.

Assessment of Quality of Life (QOL) in Obsessive Compulsive Disorder (OCD) and Dysthymic Disorder (DD): A Comparative Study

PubMed Central

Vasudev, Roopesh Gopal Nariyandada; Yallappa, Sudarshan Chikkanayakanahalli

2015-01-01

Introduction There is paucity of data on Quality of Life (QOL) in non-psychotic mental disorders. Aim To assess the Quality of Life (QOL) in Obsessive Compulsive Disorder (OCD) and compare it with Dysthymic Disorder (DD). Materials and Methods This hospital based cross-sectional study was conducted in 30 patients each diagnosed to have OCD and DD during October 2009 to September 2011 in a medical institution in Davanagere, Karnataka, India. DSM-IV-TR (Diagnostic statistical manual-IV-Text Revised) criteria was used for diagnosis of OCD and DD. Yale Brown Obsessive Compulsive Scale (Y-BOCS) and WHO QOL BREF (brief form) scales were used. Data was analysed by independent sample t-test. Results Overall QOL score was 51.07(SD=11.47) and 50.91(SD=7.41) in OCD and DD groups respectively. QOL score was comparatively low in psychological domain with score of 44.12(SD=14.14) and 45.10(12.35) in OCD and DD respectively. There was no significant difference in the QOL score of the two groups with respect to socio demographic variables in OCD group, but it was different with respect to place of residence in DD group (p<0.05). In Q1(perception on quality of life) and Q2 (perception on quality of health) domain, QOL score was marginally higher than average in both the groups. In Y-BOCS scale, no statistical significant association was found between severity of OCD and QOL score in each of the domains (p>0.05). Conclusion Overall QOL score was average and there was no significant difference of QOL score between the OCD and DD groups implying that both these non-psychotic mental disorders may have same influence or effect on QOL of the subjects. Further analytical studies will explore the associated factors of QOL in OCD and DD. PMID:26155540

Angiotensin-converting enzyme DD genotype in patients with primary pulmonary hypertension: increased frequency and association with preserved haemodynamics.

PubMed

Abraham, William T; Raynolds, Mary V; Badesch, David B; Wynne, Kristine M; Groves, Bertron M; Roden, Robert L; Robertson, Alastair D; Lowes, Brian D; Zisman, Lawrence S; Voelkel, Norbert F; Bristow, Michael R; Perryman, M Benjamin

2003-03-01

HYPOTHESIS/INTRODUCTION: A polymorphic marker within the angiotensin- converting enzyme (ACE) gene has been associated with circulating and tissue ACE activity and with a variety of forms of cardiovascular disease. Since angiotensin II (Ang II) causes pulmonary vasoconstriction and vascular and myocardial remodelling, we postulated a role for the renin-angiotensin system and the ACE DD genotype in the pathophysiology of primary pulmonary hypertension (PPH) and in the right ventricular response to pressure overload in these patients. The incidence of the ACE DD genotype was evaluated in 60 patients with severe PPH compared with two normal control populations, a group of healthy population-based controls (n=158) and subjects found suitable for cardiac organ donation (n=79). Genomic DNA extracted from peripheral leukocytes was amplified using the polymerase chain reaction to detect polymorphic markers. Haemodynamics were determined by right heart catheterisation in a subset of the PPH patients. The frequency of the ACE DD genotype was 45% in the patients with PPH, compared with 24% in the organ donors, and 28% in population-based healthy controls (p=0.01 for chi-square test). Of the 32 PPH patients with baseline haemodynamics, 12 exhibited the ACE DD genotype and 20 were non-DD. While the mean pulmonary artery pressure and the duration of symptoms attributable to pulmonary hypertension was not different between the DD and non-DD groups, cardiac output was significantly lower (3.29+0.27 vs. 5.07+0.37 L/minute, p=0.002) and the mean right atrial pressure tended to be higher (8.85+1.29 vs. 4.92+1.27 mmHg, p=0.08) in the non-DD group. The reduction in cardiac output seen in the non-DD group was not due to a difference in heart rate, but to a significant reduction in stroke volume, consistent with a decreased contractile state. In addition, non-DD patients exhibited a significantly worse functional capacity (NYHA Class 3.14+0.12 vs. 2.40+0.28, p=0.02). 1) The ACE DD genotype is significantly increased in patients with severe PPH compared with normal controls, suggesting that certain individuals may be genetically predisposed to developing pulmonary hypertension. 2) The ACE DD genotype is associated with preserved right ventricular function in PPH patients, supporting a compensatory myocardial or inotropic role for Ang II in the pressure overloaded right ventricle.

Tolstoy, Vygotsky, and the Making of Meaning.

ERIC Educational Resources Information Center

Berthoff, Ann E.

1978-01-01

Leo Tolstoy and Lev Vygotsky, like Sylvia Ashton-Warner, Maria Montessori, and Paulo Freire, base their educational philosophies on the heuristic power of language, the form-finding, and form-creating powers of the human mind. (DD)

[Molecular cloning and characterization of an acetylcholinesterase gene Dd-ace-2 from sweet potato stem nematode Ditylenchus destructor].

PubMed

Ding, Zhong; Peng, Deliang; Huang, Wenkun; He, Wenting; Gao, Bida

2008-02-01

A cDNA, named Dd-ace-2, encoding an acetylcholinesterase (AChE, EC3.1.1.7), was isolated from sweet-potato-stem nematode, Ditylenchus destructor. The nucleotide and amino acid sequences among different nematode species were compared and analyzed with DNAMAN5.0, MEGA3.0 softwares. The results showed that the complete nucleotide sequence of Dd-ace-2 gene of Ditylenchus destructor contains 2425 base pairs from which deduced 734 amino acids (GenBank accession No. EF583058). The homology rates of amino acid sequences of Dd-ace-2 gene between Ditylenchus destructor and Meloidogyne incognita, Caenorhabditis elegans, Dictyocaulus viviparous were 48.0%, 42.7%, 42.1% respectively. The mature acetylcholinesterase sequences of Ditylenchus destructor may encode by the first 701 residues of deduced 734 amino acids.The conserved motifs involved in the catalytic triad, the choline binding site and 10 aromatic residues lining the catalytic gorge were present in the Dd-ace-2 deduced protein. Phylogenetic analysis based on AChEs of other nematodes and species showed that the deduced AChE formed the same cluster with ACE-2s.

Inducible nuclear translocation of a STAT protein in Dictyostelium prespore cells: implications for morphogenesis and cell-type regulation.

PubMed

Dormann, D; Abe, T; Weijer, C J; Williams, J

2001-04-01

Dd-STATa, the Dictyostelium STAT (signal transducer and activator of transcription) protein, is selectively localised in the nuclei of a small subset of prestalk cells located in the slug tip. Injection of cAMP into the extracellular spaces in the rear of the slug induces rapid nuclear translocation of a Dd-GFP:STATa fusion protein in prespore cells surrounding the site of injection. This suggests that cAMP signals that emanate from the tip direct the localised nuclear accumulation of Dd-STATa. It also shows that prespore cells are competent to respond to cAMP, by Dd-STATa activation, and it implies that cAMP signalling is in some way limiting in the rear of the slug. Co-injection of a specific inhibitor of the cAR1 serpentine cAMP receptor almost completely prevents the cAMP-induced nuclear translocation, showing that most or all of the cAMP signal is transduced by cAR1. Dd-GFP:STATa also rapidly translocates into the nuclei of cells adjoining the front and back cut edges when a slug is bisected. Less severe mechanical disturbances, such as pricking the rear of a slug with an unfilled micropipette, also cause a more limited nuclear translocation of Dd-GFP:STATa. We propose that these signalling events form part of a repair mechanism that is activated when the migrating slug suffers mechanical damage.

The impact of comorbid dysthymic disorder on outcome in personality disorders.

PubMed

Hellerstein, David J; Skodol, Andrew E; Petkova, Eva; Xie, Hui; Markowitz, John C; Yen, Shirley; Gunderson, John; Grilo, Carlos; Daversa, Maria T; McGlashan, Thomas H

2010-01-01

The goal of our study was to investigate the impact of dysthymic disorder (DD), a form of chronic depression, on naturalistic outcome in individuals with personality disorders (PDs). The Collaborative Longitudinal Personality Disorders Study is a cohort initially including 573 subjects with 4 targeted PDs (borderline, avoidant, schizotypal, and obsessive-compulsive) and 95 subjects with major depression but no PD. At baseline, 115 subjects were diagnosed with coexisting DD, of whom 109 (94.8%) were PD subjects. Regression analyses were performed to predict 3 classes of broad clinical outcome after 2 years of prospective follow-up. We hypothesized that DD diagnosis at baseline would be associated with worse outcome on (1) persistence of a PD diagnosis, (2) impairment in psychosocial functioning (as measured by the Longitudinal Interval Follow-up Evaluation), and (3) crisis-related treatment utilization. Baseline DD diagnosis was associated with persistence of PD diagnosis at 2 years, particularly for borderline and avoidant PDs. It was associated with worse outcome on global social adjustment, life satisfaction, recreation, and friendships, but not employment or relationship with spouse. Contrary to expectation, DD did not increase suicide attempts, emergency room visits, or psychiatric hospitalizations. Comorbidity of DD is associated with persistence of PD diagnosis and with worse outcome on many, but not all, measures of psychosocial functioning. Copyright 2010 Elsevier Inc. All rights reserved.

The Impact of Comorbid Dysthymic Disorder on Outcome in Personality Disorders

PubMed Central

Hellerstein, David J.; Skodol, Andrew E.; Petkova, Eva; Xie, Hui; Markowitz, John C.; Yen, Shirley; Gunderson, John; Grilo, Carlos; Daversa, Maria T.; McGlashan, Thomas H.

2009-01-01

Objective The goal of our study was to investigate the impact of dysthymic disorder (DD), a form of chronic depression, on naturalistic outcome in individuals with personality disorders (PD). Method The Collaborative Longitudinal Personality Disorders Study is a cohort initially including 573 subjects with four targeted PDs (borderline, avoidant, schizotypal and obsessive-compulsive) and 95 subjects with major depression but no PD. At baseline, 115 subjects were diagnosed with coexisting dysthymic disorder (DD), of whom 109 (94.8%) were PD subjects. Regression analyses were performed to predict three classes of broad clinical outcome after two years of prospective follow-up. We hypothesized that DD diagnosis at baseline would be associated with worse outcome on 1) persistence of a PD diagnosis, 2) impairment in psychosocial functioning (as measured by the Longitudinal Interval Follow-up Evaluation [LIFE]), and 3) crisis-related treatment utilization. Results Baseline DD diagnosis was associated with persistence of PD diagnosis at 2 years, particularly for borderline and avoidant PDs. It was associated with worse outcome on global social adjustment, life satisfaction, recreation, and friendships, but not employment or relationship with spouse. Contrary to expectation, DD did not increase suicide attempts, emergency room visits, or psychiatric hospitalizations. Conclusions Comorbidity of DD is associated with persistence of PD diagnosis, and with worse outcome on many, but not all, measures of psychosocial functioning. PMID:20728000

Analytical validation of a reverse transcriptase droplet digital PCR (RT-ddPCR) for quantitative detection of infectious hematopoietic necrosis virus

USGS Publications Warehouse

Jia, Peng; Purcell, Maureen; Pan, Guang; Wang, Jinjin; Kan, Shifu; Liu, Yin; Zheng, Xiaocong; SHi, Xiujie; He, Junqiang; Yu, Li; Hua, Qunyi; Lu, Tikang; Lan, Wensheng; Winton, James; Jin, Ningyi; Liu, Hong

2017-01-01

Infectious hematopoietic necrosis virus (IHNV) is an important pathogen of salmonid fishes. A validated universal reverse transcriptase quantitative PCR (RT-qPCR) assay that can quantify levels of IHNV in fish tissues has been previously reported. In the present study, we adapted the published set of IHNV primers and probe for use in a reverse-transcriptase droplet digital PCR (RT-ddPCR) assay for quantification of the virus in fish tissue samples. The RT-ddPCR and RT-qPCR assays detected 13 phylogenetically diverse IHNV strains, but neither assay produced detectable amplification when RNA from other fish viruses was used. The RT-ddPCR assay had a limit of detection (LOD) equating to 2.2 plaque forming units (PFU)/μl while the LOD for the RT-qPCR was 0.2 PFU/μl. Good agreement (69.4–100%) between assays was observed when used to detect IHNV RNA in cell culture supernatant and tissues from IHNV infected rainbow trout (Oncorhynchus mykiss) and arctic char (Salvelinus alpinus). Estimates of RNA copy number produced by the two assays were significantly correlated but the RT-qPCR consistently produced higher estimates than the RT-ddPCR. The analytical properties of the N gene RT-ddPCR test indicated that this method may be useful to assess IHNV RNA copy number for research and diagnostic purposes. Future work is needed to establish the within and between laboratory diagnostic performance of the RT-ddPCR assay.

Dynamical decoupling of local transverse random telegraph noise in a two-qubit gate

NASA Astrophysics Data System (ADS)

D'Arrigo, A.; Falci, G.; Paladino, E.

2015-10-01

Achieving high-fidelity universal two-qubit gates is a central requisite of any implementation of quantum information processing. The presence of spurious fluctuators of various physical origin represents a limiting factor for superconducting nanodevices. Operating qubits at optimal points, where the qubit-fluctuator interaction is transverse with respect to the single qubit Hamiltonian, considerably improved single qubit gates. Further enhancement has been achieved by dynamical decoupling (DD). In this article we investigate DD of transverse random telegraph noise acting locally on each of the qubits forming an entangling gate. Our analysis is based on the exact numerical solution of the stochastic Schrödinger equation. We evaluate the gate error under local periodic, Carr-Purcell and Uhrig DD sequences. We find that a threshold value of the number, n, of pulses exists above which the gate error decreases with a sequence-specific power-law dependence on n. Below threshold, DD may even increase the error with respect to the unconditioned evolution, a behaviour reminiscent of the anti-Zeno effect.

In vivo immunologic selection of class I major histocompatibility complex gene deletion variants from the B16-BL6 melanoma.

PubMed

Talmadge, J E; Talmadge, C B; Zbar, B; McEwen, R; Meeker, A K; Tribble, H

1987-06-01

The mechanism by which tumor allografts escape host immunologic attack was investigated. B16-BL6 cells (the bladder 6 subline of the B16 melanoma) (H-2b) were transfected with a gene (Dd) encoding an allogeneic class I major histocompatibility complex antigen. Clones that expressed Dd antigen were injected into the footpads of nonimmune syngeneic mice, syngeneic immune mice, and nude mice. Under conditions of immunologic selection a clone that contained multiple copies of the transfected gene formed variants that lacked the transfected gene. Primary tumors and pulmonary metastases of immunized mice and pulmonary metastases of nonimmunized mice had lost the Dd gene and, in most cases, all of the associated plasmid. In contrast, in immunodeficient nude mice, primary tumors and pulmonary metastases retained the Dd gene and the associated plasmid. Deletion of genes encoding cell surface antigens may be one of the mechanisms by which allogeneic tumors escape immunologic attack.

PGL germ granule assembly protein is a base-specific, single-stranded RNase

PubMed Central

Aoki, Scott T.; Kershner, Aaron M.; Bingman, Craig A.; Wickens, Marvin; Kimble, Judith

2016-01-01

Cellular RNA-protein (RNP) granules are ubiquitous and have fundamental roles in biology and RNA metabolism, but the molecular basis of their structure, assembly, and function is poorly understood. Using nematode “P-granules” as a paradigm, we focus on the PGL granule scaffold protein to gain molecular insights into RNP granule structure and assembly. We first identify a PGL dimerization domain (DD) and determine its crystal structure. PGL-1 DD has a novel 13 α-helix fold that creates a positively charged channel as a homodimer. We investigate its capacity to bind RNA and discover unexpectedly that PGL-1 DD is a guanosine-specific, single-stranded endonuclease. Discovery of the PGL homodimer, together with previous results, suggests a model in which the PGL DD dimer forms a fundamental building block for P-granule assembly. Discovery of the PGL RNase activity expands the role of RNP granule assembly proteins to include enzymatic activity in addition to their job as structural scaffolds. PMID:26787882

78 FR 25430 - Submission for OMB review; comment request

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-01

....C. Chapter 35). DATES: Consideration will be given to all comments received by May 31, 2013. Title, Associated Form and OMB Number: DoD Active Duty/Reserve Forces Dental Exam; DD Form 2813; OMB Control Number...

Major Management Challenges and Program Risks. Department of the Treasury

DTIC Science & Technology

2001-01-01

Major Management Challenges and Program Risks Department of the TreasuryGAO-01-254 Form SF298 Citation Data Report Date ("DD MON YYYY") 00JAN2001...Report Type N/A Dates Covered (from... to) ("DD MON YYYY") Title and Subtitle Major Management Challenges and Program Risks Department of the...High Risks (GAO-01-159SP), in November 2000.This report addresses the major performance and accountability challenges facing the Department of the

Major Management Challenges and Program Risks. Department of Labor

DTIC Science & Technology

2001-01-01

Major Management Challenges and Program Risks Department of LaborGAO-01-251 Form SF298 Citation Data Report Date ("DD MON YYYY") 00JAN2001 Report...Type N/A Dates Covered (from... to) ("DD MON YYYY") Title and Subtitle Major Management Challenges and Program Risks Department of Labor Contract or... Challenges and High Risks (GAO-01-159SP), in November 2000. This 2001 Performance and Accountability Series contains separate reports on 21 agencies

Major Management Challenges and Program Risks: Small Business Administration

DTIC Science & Technology

2001-01-01

2001 Major Management Challenges and Program Risks Small Business AdministrationGAO-01-260 Form SF298 Citation Data Report Date ("DD MON YYYY...34) 00JAN2001 Report Type N/A Dates Covered (from... to) ("DD MON YYYY") Title and Subtitle Major Management Challenges and Program Risks Small Business ...Administration (SBA) as it seeks to aid, counsel, assist, and protect the interests of the nations small businesses and help businesses and families

Fiscal Year (FY) 2001 Budget Estimates. Army National Guard. Military Construction Program FY 2001. Justification Data Submission to Congress. Department of the Army.

DTIC Science & Technology

2000-02-01

CONSIDERATIONS ix-xx FUTURE YEARS DEFENSE PLAN AUDIT TRAIL xxi-xxiii PROJECT JUSTIFICATION DOCUMENTS 1-149 PLANNING & DESIGN JUSTIFICATION DOCUMENT 15 0...OUTSTANDING POLUTION AND SAFETY DEFICIENCIES: DDl°™1390s2 MAY 78 1. COMPONENT FY 2001 GUARD AND RESERVE 2. DATE ARNG MILITARY CONSTRUCTION FEB00 3...TOTAL 36 286 13 MAJOR EQUIPMENT AND AIRCRAFT TYPE AUTHORIZED ASSIGNED 14. OUTSTANDING POLUTION AND SAFETY DEFICIENCIES: DD:A O™1390S2 1

Application of diffuse discharges of atmospheric pressure formed by runaway electrons for modification of copper and stainless steel surface

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tarasenko, V. F., E-mail: VFT@loi.hcei.tsc.ru; Shulepov, M. A.; Erofeev, M. V.

The results of studies devoted to the influence of a runaway electron pre-ionized diffuse discharge (REP DD) formed in air and nitrogen at atmospheric pressure on the surface of copper and stainless steel are presented. Nanosecond high-voltage pulses were used to obtain REP DD in different gases at high pressures in a chamber with a flat anode and a cathode possessing a small radius of curvature. This mode of discharge was implemented owing to the generation of runaway electrons and X-rays. The conditions under which the surface of copper and stainless steel was cleaned from carbon and oxidized are described.

Whole exome sequencing is necessary to clarify ID/DD cases with de novo copy number variants of uncertain significance: Two proof-of-concept examples.

PubMed

Giorgio, Elisa; Ciolfi, Andrea; Biamino, Elisa; Caputo, Viviana; Di Gregorio, Eleonora; Belligni, Elga Fabia; Calcia, Alessandro; Gaidolfi, Elena; Bruselles, Alessandro; Mancini, Cecilia; Cavalieri, Simona; Molinatto, Cristina; Cirillo Silengo, Margherita; Ferrero, Giovanni Battista; Tartaglia, Marco; Brusco, Alfredo

2016-07-01

Whole exome sequencing (WES) is a powerful tool to identify clinically undefined forms of intellectual disability/developmental delay (ID/DD), especially in consanguineous families. Here we report the genetic definition of two sporadic cases, with syndromic ID/DD for whom array-Comparative Genomic Hybridization (aCGH) identified a de novo copy number variant (CNV) of uncertain significance. The phenotypes included microcephaly with brachycephaly and a distinctive facies in one proband, and hypotonia in the legs and mild ataxia in the other. WES allowed identification of a functionally relevant homozygous variant affecting a known disease gene for rare syndromic ID/DD in each proband, that is, c.1423C>T (p.Arg377*) in the Trafficking Protein Particle Complex 9 (TRAPPC9), and c.154T>C (p.Cys52Arg) in the Very Low Density Lipoprotein Receptor (VLDLR). Four mutations affecting TRAPPC9 have been previously reported, and the present finding further depicts this syndromic form of ID, which includes microcephaly with brachycephaly, corpus callosum hypoplasia, facial dysmorphism, and overweight. VLDLR-associated cerebellar hypoplasia (VLDLR-CH) is characterized by non-progressive congenital ataxia and moderate-to-profound intellectual disability. The c.154T>C (p.Cys52Arg) mutation was associated with a very mild form of ataxia, mild intellectual disability, and cerebellar hypoplasia without cortical gyri simplification. In conclusion, we report two novel cases with rare causes of autosomal recessive ID, which document how interpreting de novo array-CGH variants represents a challenge in consanguineous families; as such, clinical WES should be considered in diagnostic testing. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

U.S. Department of Agriculture: Resolution of Discrimination Complaints Involving Farm Credit and Payment Programs

DTIC Science & Technology

2001-04-12

2There have also been long-standing concerns about USDA’s treatment of minority employees . As requested, however, this report focuses on program -related... program participants and employees . Form SF298 Citation Data Report Date ("DD MON YYYY") 00MAY2001 Report Type N/A Dates Covered (from... to) ("DD MON...Indianswho were denied benefits under the U.S. Department of Agricultures (USDA) farm assistance programs 1 have been a long-standing issue. Compounding

Coast Guard: Progress Being Made on Deepwater Project, but Risks Remain

DTIC Science & Technology

2001-05-01

Risks Remain GAO-01-564 Form SF298 Citation Data Report Date ("DD MON YYYY") 00MAY2001 Report Type N/A Dates Covered (from... to) ("DD MON YYYY...34) Title and Subtitle COAST GUARD: Progress Being Made on Deepwater Project, but Risks Remain Contract or Grant Number Program Element Number Authors...for this project. As agreed with your office, this report focuses on the major risks facing the Subject Terms Document Classification unclassified

VETERANS BENEFITS: Veterans Have Mixed Views on a Lump Sum Disability Payment Option

DTIC Science & Technology

2000-12-01

These advantages and disadvantages generally weigh the benefit of financial flexibility against the risk of financial loss. 8If the lump sum is... BENEFITS Veterans Have Mixed Views on a Lump Sum Disability Payment OptionGAO-01-172 Form SF298 Citation Data Report Date ("DD MON YYYY") 00DEC2000...Report Type N/A Dates Covered (from... to) ("DD MON YYYY") Title and Subtitle VETERANS BENEFITS Veterans Have Mixed Views on a Lump Sum Disability

Cognitive behavioral hypnotherapy for dissociative disorders.

PubMed

Fine, Catherine G

2012-04-01

Dissociative disorders (DD) prevail as sequelae to overwhelming experiences in childhood. These readily formed post-traumatic responses and trance states develop in high hypnotizable subjects whose dysregulations become organized into ego states. A cognitive behavioral hypnotherapeutic treatment model will effectively contain, explore, metabolize, and resolve these life-endangering conditions. This article will detail the cognitive hypnotic world of DD patients, the relational spaces of the ego states, and the triphasic treatment mode to successfully resolve the dissociative pathology. Structured and phase appropriate hypnotic interventions will be described.

Evaluation of the Clinical Data Dictionary (CiDD)

PubMed Central

Lee, Myung Kyung; Min, Yul Ha; Kim, Younglan; Min, Hyo Ki; Ham, Sung Woo

2010-01-01

Objectives The purpose of the study was to evaluate content coverage and data quality of the Clinical Data Dictionary (CiDD) developed by the Center for Interoperable EHR (CiEHR). Methods A total of 12,994 terms were collected from 98 clinical forms of a tertiary cancer center hospital with 500 beds. After data cleaning, 9,418 terms were mapped with the data items of the CiDD by the research team, and validated by 30 doctors and nurses at the research hospital. Results Mapping results were classified into five categories: lexically mapped; semantically mapped; mapped to either a broader term or a narrower term; mapped to more than one term and not mapped. In terms of coverage, out of 9,418 terms, 6,750 (71.7%) terms were mapped; 4,319 (45.9%) terms were lexically mapped; 2,431 (25.8%) were semantically mapped; 281 (3.0%) terms were mapped to a broader term; 43 (0.5%) were mapped to a narrower term; and 550 (5.8%) were mapped to more than one term. In terms of data quality, the CiDD has problems such as errors in concept namingand representation, redundancy in synonyms, inadequate synonyms, and ambiguity in meaning. Conclusions Although the CiDD has terms covering 72% of local clinical terms, the CiDD can be improved by cleaning up errors and redundancies, adding textual definitions or use cases of the concept, and arranging the concepts in a hierarchy. PMID:21818428

The Dictyostelium MAP kinase kinase DdMEK1 regulates chemotaxis and is essential for chemoattractant-mediated activation of guanylyl cyclase.

PubMed Central

Ma, H; Gamper, M; Parent, C; Firtel, R A

1997-01-01

We have identified a MAP kinase kinase (DdMEK1) that is required for proper aggregation in Dictyostelium. Null mutations produce extremely small aggregate sizes, resulting in the formation of slugs and terminal fruiting bodies that are significantly smaller than those of wild-type cells. Time-lapse video microscopy and in vitro assays indicate that the cells are able to produce cAMP waves that move through the aggregation domains. However, these cells are unable to undergo chemotaxis properly during aggregation in response to the chemoattractant cAMP or activate guanylyl cyclase, a known regulator of chemotaxis in Dictyostelium. The activation of guanylyl cyclase in response to osmotic stress is, however, normal. Expression of putative constitutively active forms of DdMEK1 in a ddmek1 null background is capable, at least partially, of complementing the small aggregate size defect and the ability to activate guanylyl cyclase. However, this does not result in constitutive activation of guanylyl cyclase, suggesting that DdMEK1 activity is necessary, but not sufficient, for cAMP activation of guanylyl cyclase. Analysis of a temperature-sensitive DdMEK1 mutant suggests that DdMEK1 activity is required throughout aggregation at the time of guanylyl cyclase activation, but is not essential for proper morphogenesis during the later multicellular stages. The activation of the MAP kinase ERK2, which is essential for chemoattractant activation of adenylyl cyclase, is not affected in ddmek1 null strains, indicating that DdMEK1 does not regulate ERK2 and suggesting that at least two independent MAP kinase cascades control aggregation in Dictyostelium. PMID:9250676

Maternal Stress and Coping Strategies in Developmental Dyslexia: An Italian Multicenter Study.

PubMed

Carotenuto, Marco; Messina, Antonietta; Monda, Vincenzo; Precenzano, Francesco; Iacono, Diego; Verrotti, Alberto; Piccorossi, Alessandra; Gallai, Beatrice; Roccella, Michele; Parisi, Lucia; Maltese, Agata; Lavano, Francesco; Marotta, Rosa; Lavano, Serena Marianna; Lanzara, Valentina; Ferrentino, Roberta Ida; Pisano, Simone; Salerno, Margherita; Valenzano, Anna; Triggiani, Antonio Ivano; Polito, Anna N; Cibelli, Giuseppe; Monda, Marcellino; Messina, Giovanni; Ruberto, Maria; Esposito, Maria

2017-01-01

Studies about the impact of developmental dyslexia (DD) on parenting are scarce. Our investigation aimed to assess maternal stress levels and mothers' copying styles in a population of dyslexic children. A total of 874 children (500 boys, 374 girls; mean age 8.32 ± 2.33 years) affected by DD was included in the study. A total of 1,421 typically developing children (789 boys, 632 girls; mean age 8.25 ± 3.19 years) were recruited from local schools of participating Italian Regions (Abruzzo, Calabria, Campania, Puglia, Umbria, Sicily) and used as control-children group. All mothers (of both DD and typically developing children) filled out an evaluation for parental stress (Parenting Stress Index-Short Form) and coping strategies [Coping Inventory for Stressful Situations (CISS)]. No statistical differences for mean age ( p  = 0.456) and gender ( p  = 0.577) were found between DD and control children. Mothers of children affected by DD showed an higher rate of all parental stress indexes (Parental Distress domain p  < 0.001, Difficult Child p  < 0.001, Parent-Child Dysfunctional Interaction p  < 0.001, and Total Stress subscale score p  < 0.001) than controls mothers. According to the CISS evaluation, mothers of DD children reported a significantly higher rate of emotion-oriented ( p  < 0.001) and avoidance-oriented ( p  < 0.001) coping styles than mothers of typical developing children. On the other hand, a lower representation of task-oriented coping style was found in mothers of DD children ( p  < 0.001) in comparison to mothers of control-children. Our study shows the clinical relevance of the burden carried by the mothers of children affected by DD and suggests the importance to assess parents, particularly mothers, to improve family compliance and clinical management of this disorder.

Frequency of Depressive Syndromes in Elderly Individuals with No Cognitive Impairment, Mild Cognitive Impairment, and Alzheimer's Disease Dementia in a Memory Clinic Setting.

PubMed

Lee, Jun Ho; Byun, Min Soo; Yi, Dahyun; Choe, Young Min; Choi, Hyo Jung; Baek, Hyewon; Sohn, Bo Kyung; Kim, Hyun Jung; Lee, Younghwa; Woo, Jong Inn; Lee, Dong Young

2016-01-01

The aims of this study were to investigate the frequency of various depressive syndromes in elderly individuals with no cognitive impairment (NC), mild cognitive impairment (MCI), and Alzheimer's disease dementia (AD) in a memory clinic setting, and then to test whether severe and milder forms of depressive syndromes are differentially associated with the cognitive groups. For 216 NC, 478 MCI, and 316 AD subjects, we investigated the frequency of depressive syndromes, defined by three different categories: major and minor depressive disorder (MaDD and MiDD) according to the Diagnostic and Statistical Manual of Mental Disorders, 4th edition, as well as depression according to the National Institute of Mental Health provisional diagnostic criteria for depression in Alzheimer's disease (NIMH-dAD). The frequency of MaDD did not show any significant difference among NC, MCI, and AD. In contrast, the frequencies of MiDD and NIMH-dAD were higher than those of MaDD and showed significant group differences with a gradual increase from NC to AD. The findings suggest that the degenerative process of Alzheimer's disease contributes to the occurrence of mild depressive conditions, but not to severe depression. © 2016 S. Karger AG, Basel.

The three-year naturalistic course of major depressive disorder, dysthymic disorder and double depression.

PubMed

Rhebergen, Didi; Beekman, Aartjan Tf; Graaf, Ron de; Nolen, Willem A; Spijker, Jan; Hoogendijk, Witte J; Penninx, Brenda Wjh

2009-06-01

Recent studies support a distinction between acute and chronic forms of depression, which contrasts the single-disease hypothesis for depressive disorders. Insight into the (determinants of) the 3-year naturalistic course of major depressive disorder (MDD), dysthymic disorder (Dysth) and double depression (DD) may contribute to this debate. Data were derived from NEMESIS, an epidemiologic survey in the adult population of the Netherlands. 400 Respondents who met the Composite International Diagnostic Interview (CIDI) criteria of MDD and/or Dysth were selected. Cox proportional hazards analyses and Linear Mixed Models were conducted to examine 3-year course trajectories of MDD, Dysth and DD and determinants for course. Adjusted analyses showed similar course trajectories for Dysth and DD, which were significantly worse than the course for MDD. Determinants of unfavorable course were neuroticism and poor functioning. Attrition was higher among persons with Dysth. However, since attrition is generally associated with poorer outcome, this would indicate that differences in course may even have been larger in reality. Dysth and DD involve a similar course which is worse than the course of MDD only. These results do not support a distinction between Dysth and DD. Duration of symptoms and level of functioning may serve as two clinically relevant classifying dimensions within the broad category of depressive disorders.

Rifamycin action on RNA polymerase in antibiotic-tolerant Mycobacterium tuberculosis results in differentially detectable populations.

PubMed

Saito, Kohta; Warrier, Thulasi; Somersan-Karakaya, Selin; Kaminski, Lina; Mi, Jianjie; Jiang, Xiuju; Park, Suna; Shigyo, Kristi; Gold, Ben; Roberts, Julia; Weber, Elaina; Jacobs, William R; Nathan, Carl F

2017-06-13

Mycobacterium tuberculosis (Mtb) encounters stresses during the pathogenesis and treatment of tuberculosis (TB) that can suppress replication of the bacteria and render them phenotypically tolerant to most available drugs. Where studied, the majority of Mtb in the sputum of most untreated subjects with active TB have been found to be nonreplicating by the criterion that they do not grow as colony-forming units (cfus) when plated on agar. However, these cells are viable because they grow when diluted in liquid media. A method for generating such "differentially detectable" (DD) Mtb in vitro would aid studies of the biology and drug susceptibility of this population, but lack of independent confirmation of reported methods has contributed to skepticism about their existence. Here, we identified confounding artifacts that, when avoided, allowed development of a reliable method of producing cultures of ≥90% DD Mtb in starved cells. We then characterized several drugs according to whether they contribute to the generation of DD Mtb or kill them. Of the agents tested, rifamycins led to DD Mtb generation, an effect lacking in a rifampin-resistant strain with a mutation in rpoB , which encodes the canonical rifampin target, the β subunit of RNA polymerase. In contrast, thioridazine did not generate DD Mtb from starved cells but killed those generated by rifampin.

Pattern formation in Dictyostelium discoideum aggregates in confined microenvironments

NASA Astrophysics Data System (ADS)

Hallou, Adrien; Hersen, Pascal; di Meglio, Jean-Marc; Kabla, Alexandre

Dictyostelium Discoideum (Dd) is often viewed as a model system to study the complex collective cell behaviours which shape an embryo. Under starvation, Dd cells form multicellular aggregates which soon elongate, starting to display an anterior-posterior axis by differentiating into two distinct cell populations; prestalk (front) and prespore (rear) cells zones. Different models, either based on positional information or on differentiation followed up by cell sorting, have been proposed to explain the origin and the regulation of this spatial pattern.To decipher between the proposed hypotheses, we have developed am experimental platform where aggregates, made of genetically engineered Dd cells to express fluorescent reporters of cell differentiation in either prestalk or prespore cells, are allowed to develop in 20 to 400 μm wide hydrogel channels. Such a setup allows us to both mimic Dd confined natural soil environment and to follow the patterning dynamics using time-lapse microscopy. Tracking cell lineage commitments and positions in space and time, we demonstrate that Dd cells differentiate first into prestalk and prespore cells prior to sorting into an organized spatial pattern on the basis of collective motions based on differential motility and adhesion mechanisms. A. Hallou would like to thank the University of Cambridge for the Award of an ``Oliver Gatty Studentship in Biophysical and Colloid Science''.

[Comorbidity and related costs as a burden in the treatment of outpatients with depressive disorders in a primary care setting].

PubMed

Sicras Mainar, A; Navarro Artieda, R; Rejas Gutiérrez, J; Blanca Tamayo, M; Serrat Tarrés, J; Llopart López, J R

2007-01-01

To study the impact of depressive disorders (DD) on health care expenditure and to measure associated comorbidity in patients in primary care settings (PCS) under normal clinical practice conditions. A retrospective cohort study was carried out. The study cohort consisted of outpatients aged over 14 years of age with an established diagnosis of DD (ICPC; P76) treated in a PC health centre during 2004. A comparative cohort was formed with the remaining outpatients without DD, treated in that health centre. Main factors for calculation were: age, gender, history/comorbidity and health resource use and the corresponding outpatient costs; drugs, diagnostic tests, visits to specialists and PC physicians. Multiple logistic regression analysis and ANCOVA models were used in order to adjust costs and comorbidities between the cohorts of patients. A total of 64,072 subjects were assessed; 6,592 patients with DD [10.3% (CI: 8.2-12.4%), 74.5% (CI: 73.4%-75.6%) females]. DD outpatients displayed a higher number of episodes of comorbidities/year (mean +/- SD; 7.4 +/- 4.3 vs. 4.7 +/- 3.3, p < 0.0001) and global medical visits/patient/year (12.0 +/- 9.3 vs. 7.4 +/- 7.6, p < 0.0001). The main comorbidities associated to DD were neurological disorders [Odds ratio (95% CI); 2.1 (CI: 1.5-2.6), p < 0.0001], alcoholism [1.6 (CI: 1.3-1.9), p < 0.0001] and malignancies [1.3 (CI: 1.1-1.5), p < 0.0001]. DD were associated with significantly higher adjusted total costs; 1,083.8 euro (SEM; 8.4 euro) vs. 684.1 euro (3.4 euro), p < 0.0001. Higher costs were displayed for elderly patients. Sixty-two percent of the total cost was related to drugs. Prevalence of DD was higher, particularly in women. Following adjustment in accordance with comorbidity, age and sex, DD outpatients used more health care resources and implied higher costs. Higher costs were associated with age.

Using Simulation to Determine a Strategy for Positively Tracking a Cruise Missile by CMMCA

DTIC Science & Technology

1990-03-01

ANPOS/SS(6),ATC/SS(7),ATAS/ SS(8),ABANGLE/SS(9),CBANGLE/DD(1),CEVEL/ DD(2),CNVEL/DD(3),CTCC/DD(4),AEVEL/ DD(5), ANVEL /DD(6),ATCC RECORD,TNOW,MINUTES,20...ABANGLE),(SS(9),CBANGLE), & (DD(1),CEVEL),(DD(2),CNVEL).(DD(3),CTCC), & (DD(4),AEVEL),(DD(5), ANVEL ),(DD(6).ATCC) GOTO(l,2,3,4,5,6) ,I 1IF (XX(14) *EQ. 1...ABANGLE),(SS(9),CBANGLE), & (DD(l),CEVEL),(DD(2),CNVEL),(DD(3),CTCG), & (DD(4),AEVEL),(DD(5), ANVEL ),CDD(6),ATCC) REAL COSD EXTERNAL COSD REAL SIND

The role of density-dependent and -independent processes in spawning habitat selection by salmon in an Arctic riverscape.

PubMed

Huntsman, Brock M; Falke, Jeffrey A; Savereide, James W; Bennett, Katrina E

2017-01-01

Density-dependent (DD) and density-independent (DI) habitat selection is strongly linked to a species' evolutionary history. Determining the relative importance of each is necessary because declining populations are not always the result of altered DI mechanisms but can often be the result of DD via a reduced carrying capacity. We developed spatially and temporally explicit models throughout the Chena River, Alaska to predict important DI mechanisms that influence Chinook salmon spawning success. We used resource-selection functions to predict suitable spawning habitat based on geomorphic characteristics, a semi-distributed water-and-energy balance hydrologic model to generate stream flow metrics, and modeled stream temperature as a function of climatic variables. Spawner counts were predicted throughout the core and periphery spawning sections of the Chena River from escapement estimates (DD) and DI variables. Additionally, we used isodar analysis to identify whether spawners actively defend spawning habitat or follow an ideal free distribution along the riverscape. Aerial counts were best explained by escapement and reference to the core or periphery, while no models with DI variables were supported in the candidate set. Furthermore, isodar plots indicated habitat selection was best explained by ideal free distributions, although there was strong evidence for active defense of core spawning habitat. Our results are surprising, given salmon commonly defend spawning resources, and are likely due to competition occurring at finer spatial scales than addressed in this study.

The role of density-dependent and –independent processes in spawning habitat selection by salmon in an Arctic riverscape

DOE PAGES

Huntsman, Brock M.; Falke, Jeffrey A.; Savereide, James W.; ...

2017-05-22

Density-dependent (DD) and density-independent (DI) habitat selection is strongly linked to a species’ evolutionary history. Determining the relative importance of each is necessary because declining populations are not always the result of altered DI mechanisms but can often be the result of DD via a reduced carrying capacity. Here, we developed spatially and temporally explicit models throughout the Chena River, Alaska to predict important DI mechanisms that influence Chinook salmon spawning success. We used resource-selection functions to predict suitable spawning habitat based on geomorphic characteristics, a semi-distributed water-and-energy balance hydrologic model to generate stream flow metrics, and modeled stream temperaturemore » as a function of climatic variables. Spawner counts were predicted throughout the core and periphery spawning sections of the Chena River from escapement estimates (DD) and DI variables. In addition, we used isodar analysis to identify whether spawners actively defend spawning habitat or follow an ideal free distribution along the riverscape. Aerial counts were best explained by escapement and reference to the core or periphery, while no models with DI variables were supported in the candidate set. Moreover, isodar plots indicated habitat selection was best explained by ideal free distributions, although there was strong evidence for active defense of core spawning habitat. These results are surprising, given salmon commonly defend spawning resources, and are likely due to competition occurring at finer spatial scales than addressed in this study.« less

The role of density-dependent and –independent processes in spawning habitat selection by salmon in an Arctic riverscape

USGS Publications Warehouse

Huntsman, Brock M.; Falke, Jeffrey A.; Savereide, James W.; Bennett, Katrina E.

2017-01-01

Density-dependent (DD) and density-independent (DI) habitat selection is strongly linked to a species’ evolutionary history. Determining the relative importance of each is necessary because declining populations are not always the result of altered DI mechanisms but can often be the result of DD via a reduced carrying capacity. We developed spatially and temporally explicit models throughout the Chena River, Alaska to predict important DI mechanisms that influence Chinook salmon spawning success. We used resource-selection functions to predict suitable spawning habitat based on geomorphic characteristics, a semi-distributed water-and-energy balance hydrologic model to generate stream flow metrics, and modeled stream temperature as a function of climatic variables. Spawner counts were predicted throughout the core and periphery spawning sections of the Chena River from escapement estimates (DD) and DI variables. Additionally, we used isodar analysis to identify whether spawners actively defend spawning habitat or follow an ideal free distribution along the riverscape. Aerial counts were best explained by escapement and reference to the core or periphery, while no models with DI variables were supported in the candidate set. Furthermore, isodar plots indicated habitat selection was best explained by ideal free distributions, although there was strong evidence for active defense of core spawning habitat. Our results are surprising, given salmon commonly defend spawning resources, and are likely due to competition occurring at finer spatial scales than addressed in this study.

Increased frequency of angiotensin-converting enzyme DD genotype in Saudi overweight and obese patients.

PubMed

El-Hazmi, Mohsen Af; Warsy, Arjumand S

2003-01-01

Several studies have been carried out to investigate the insertion/deletion (I/D) polymorphism of angiotensin-converting enzyme (ACE) in obese and normal weight individuals, often with contradictory results in different populatios. In some studies, the DD genotype occurs at a high frequency in obesity, while in others no association has been demonstrated. Since obesity and overweight are of frequent occurrence in the Saudi population, we initiated this study to investigate the I/D polymorphism of ACE in obese, overweight and normal weight Saudis. The study group included 457 Saudi males and females. The height and weight were measured and body mass index (BMI) was calculated. Based on the BMI value, the total study population was classified as normal, overweight and obese. A total of 117 obese (BMI >30 (kg/m(2))), 185 overweight (BMI=25-29.9 (kg/m(2))) and 155 normal weight individuals (BMI< (kg/m (2)) were enrolled. Polymerase chain reaction (PCR) and agarose gel electrophoresis were used to study the ACE polymorphism. For the deletion (D) form, a 190 bp and for the insertion (I) form, 490 bp DNA fragment were obtained on 2% agarose gel electrophoresis. The frequency of DD genotype was 76.9% in the obese, and 73.5% in the overweight individuals, compared to 19.66% and 24.86% of the ID genotype in the obese and overweight, respectively. Among the normal weight individuals, the frequency was significantly lower for DD and higher for the ID genotype, i.e., 58.7% for DD and 40% for ID. The allele frequencies in the obese were 0.867 and 0.133 for the D and I alleles, respectively. While in the overweight, the frequencies were 0.859 and 0.141 for these alleles, respectively, compared to 0.787 and 0.213 in the normal weight individuals. The DD genotype and D allele occur at a high frequency in Saudi overweight and obese individuals, and may have some role in fat accumulation by affecting metabolic pathways of fat, thus leading to the development of overweight and obesity.

Penicillin-binding protein 4 of Escherichia coli: molecular cloning of the dacB gene, controlled overexpression, and alterations in murein composition.

PubMed

Korat, B; Mottl, H; Keck, W

1991-03-01

The penicillin-binding protein 4 (PBP4), from Escherichia coli, a DD-carboxypeptidase/DD-endopeptidase, was purified in an enzymatically active form to homogeneity by affinity chromatography on 6-aminopenicillanic acid/Sepharose and heparin/Sepharose. Polyclonal antibodies raised against the pure protein were used to identify and isolate PBP4 overproducing clones from an E. coli expression library, which was established on the basis of a temperature-inducible runaway replication plasmid. Three positive clones were isolated, one of which carried the intact structural gene dacB that codes for PBP4, on a 1.9kb SmaI-EcoRI fragment, whereas the other two carried truncated forms of this gene. The direction of transcription was determined. The PBP4 overproducing strain, when grown in rich medium, tolerated 160-fold overexpression. After disrupting cells by sonication, the majority (80%) of the overproduced PBP4 was detected in the 100,000 X g supernatant. Southern blotting analysis using the cloned dacB gene as a probe revealed that, in contrast to that described by Takeda et al. (1981), the plasmid pLC18-38 of the Clarke-Carbon collection does not code for PBP4. The overall composition of murein, synthesized in vitro or in vivo by the PBP4 overproducing strain, as determined by high-performance liquid chromatography analysis, suggests that PBP4 is not involved in transpeptidation but exclusively catalyses a DD-carboxypeptidase and DD-endopeptidase reaction.

32 CFR Appendix A to Part 636 - References

Code of Federal Regulations, 2011 CFR

2011-07-01

... INVESTIGATIONS MOTOR VEHICLE TRAFFIC SUPERVISION (SPECIFIC INSTALLATIONS) Pt. 636, App. A Appendix A to Part 636... 3946, Military Police Traffic Accident Report DA Form 3975, Military Police Report DD Form 1920... Understanding, Subject: Seizure of Assets for Administrative Forfeiture in Drug Related Cases. ...

32 CFR Appendix A to Part 636 - References

Code of Federal Regulations, 2010 CFR

2010-07-01

... INVESTIGATIONS MOTOR VEHICLE TRAFFIC SUPERVISION (SPECIFIC INSTALLATIONS) Pt. 636, App. A Appendix A to Part 636... 3946, Military Police Traffic Accident Report DA Form 3975, Military Police Report DD Form 1920... Understanding, Subject: Seizure of Assets for Administrative Forfeiture in Drug Related Cases. ...

32 CFR 842.5 - Claims forms.

Code of Federal Regulations, 2010 CFR

2010-07-01

... certain is sufficient to file a claim. The claimant should use these forms when filing a claim: (a) Claim... of or Damage to Personal Property Incident to Service, or DD Forms 1842, Claim for Personal Property Against the United States, and 1844, Schedule of Property and Claim Analysis Chart, to file the claim. (b...

32 CFR 842.5 - Claims forms.

Code of Federal Regulations, 2011 CFR

2011-07-01

... certain is sufficient to file a claim. The claimant should use these forms when filing a claim: (a) Claim... of or Damage to Personal Property Incident to Service, or DD Forms 1842, Claim for Personal Property Against the United States, and 1844, Schedule of Property and Claim Analysis Chart, to file the claim. (b...

Ultrafast Spectroscopy of Chromophores

DTIC Science & Technology

2012-01-31

REPORT DOCUMENTATION PAGE Form Approved OMB No. 0704-0188 Public reporting burden for this collection of information is estimated to average 1 ...currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. 1 . REPORT DATE (DD-MM-YYYY) 2. REPORT TYPE 3. DATES... 1

77 FR 17461 - Proposed Reinstatement; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-26

... forms of information technology. DATES: Consideration will be given to all comments received by May 25... of DD Form 2345. Affected Public: Individuals or households; businesses or other for profit; not-for... restricted databases and obstructs conference attendance where restricted data will be discussed. Dated...

48 CFR 1845.7102 - Instructions for preparing DD Form 1419.

Code of Federal Regulations, 2010 CFR

2010-10-01

... AND SPACE ADMINISTRATION CONTRACT MANAGEMENT GOVERNMENT PROPERTY Forms Preparation 1845.7102... item requested. Block 3. Enter the manufacturer's model style, or catalog number assigned to the equipment being requisitioned. Always use the model number, if available. The style number is the next...

Ada Compiler Validation Summary Report: Certificate Number 890627W1. 10103 Harris Corporation, Computer Systems Division, Harris Ada, Version 5.0 Harris H1000

DTIC Science & Technology

1989-06-27

Department of Defense Washington DC 20301-3081 Ada Compiler Validation Summary Report : Compiler Name: Harris Ada, Version 5.0 Certificate Number...890627W1.10103 Host: Harris HIOO0 under VOS, E.i Target: Harris HiO00 under VOS, E.1 Testing Completed June 27, 1989 using ACVC 1.10 This report has been...arris Corporation, Computer Systems Division Harris Ada, Version 5.0, Harris H1000 under VOS, 8.1 (Host & Target), Wright-Patterson AFB, ACVC 1.10 DD

National Defense Budget Estimates for FY 1990/1991

DTIC Science & Technology

1989-03-01

but have no effect on TOA. These are collections from the public that arise out of the business-type or market -oriented activities of the Government...magnitude and direction of changes in supply and demand in various markets . There are many different measures of inflation: a. GNP Implicit Price...m a Dmo 0 o~o 0 NCDC wm~ 0 CMI-r- CC40~ ’jU.", 101,0. >a% r- Ln -m I*CN Ln LA CO -4 M m 04 w-4 to 0 C Dr- CN c * -m oNLn -,r mm m -ddL -ia a o Nr, r

Rifamycin action on RNA polymerase in antibiotic-tolerant Mycobacterium tuberculosis results in differentially detectable populations

PubMed Central

Saito, Kohta; Warrier, Thulasi; Somersan-Karakaya, Selin; Kaminski, Lina; Mi, Jianjie; Jiang, Xiuju; Park, Suna; Shigyo, Kristi; Gold, Ben; Roberts, Julia; Weber, Elaina; Jacobs, William R.; Nathan, Carl F.

2017-01-01

Mycobacterium tuberculosis (Mtb) encounters stresses during the pathogenesis and treatment of tuberculosis (TB) that can suppress replication of the bacteria and render them phenotypically tolerant to most available drugs. Where studied, the majority of Mtb in the sputum of most untreated subjects with active TB have been found to be nonreplicating by the criterion that they do not grow as colony-forming units (cfus) when plated on agar. However, these cells are viable because they grow when diluted in liquid media. A method for generating such “differentially detectable” (DD) Mtb in vitro would aid studies of the biology and drug susceptibility of this population, but lack of independent confirmation of reported methods has contributed to skepticism about their existence. Here, we identified confounding artifacts that, when avoided, allowed development of a reliable method of producing cultures of ≥90% DD Mtb in starved cells. We then characterized several drugs according to whether they contribute to the generation of DD Mtb or kill them. Of the agents tested, rifamycins led to DD Mtb generation, an effect lacking in a rifampin-resistant strain with a mutation in rpoB, which encodes the canonical rifampin target, the β subunit of RNA polymerase. In contrast, thioridazine did not generate DD Mtb from starved cells but killed those generated by rifampin. PMID:28559332

Authoritative parenting and parental stress in parents of pre-school and older children with developmental disabilities.

PubMed

Woolfson, L; Grant, E

2006-03-01

Rearing a child with a developmental disability is associated with increased parental stress. Theories of stress and adjustment and bi-directional theories of child development suggest that parenting could influence these negative outcomes. Relationships between parenting approaches and stress in parents of children with developmental disabilities (DD) (N = 53) were examined across two age groups, 3-5 years and 9-11 years and compared with a contrast group of typically developing children (TD) (N = 60). Measures used were the Parenting Stress Index-Short Form and Rickel and Biasatti's modification of Block's Child Rearing Practices Report, classified into Baumrind's parenting styles using Reitman and Gross's method. Parents in the older DD group used Authoritative parenting less than parents in the younger DD group, while the opposite developmental pattern was seen in the TD group. Multivariate analysis of variance showed a significant group x parenting style interaction for Parental Distress, Parent-Child Dysfunctional Interaction and Difficult Child. Stress measures were higher for the DD group and seemed to be associated with Authoritative parenting approaches, an effect that was not observed in the TD group. Findings suggest that the well-established effect of group on stress may be moderated by parenting style. Authoritative parenting may be highly stressful for parents of children with DD to implement, resulting in a decrease in its use across the two age groups.

32 CFR 323.6 - Forms and reports.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 2 2012-07-01 2012-07-01 false Forms and reports. 323.6 Section 323.6 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) PRIVACY PROGRAM DEFENSE LOGISTICS AGENCY PRIVACY PROGRAM § 323.6 Forms and reports. DLA activities may be required to...

32 CFR 323.6 - Forms and reports.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 2 2010-07-01 2010-07-01 false Forms and reports. 323.6 Section 323.6 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) PRIVACY PROGRAM DEFENSE LOGISTICS AGENCY PRIVACY PROGRAM § 323.6 Forms and reports. DLA activities may be required to...

32 CFR 323.6 - Forms and reports.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 2 2011-07-01 2011-07-01 false Forms and reports. 323.6 Section 323.6 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) PRIVACY PROGRAM DEFENSE LOGISTICS AGENCY PRIVACY PROGRAM § 323.6 Forms and reports. DLA activities may be required to...

32 CFR 1288.6 - Forms and reports.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 6 2010-07-01 2010-07-01 false Forms and reports. 1288.6 Section 1288.6 National Defense Other Regulations Relating to National Defense DEFENSE LOGISTICS AGENCY MISCELLANEOUS REGISTRATION OF PRIVATELY OWNED MOTOR VEHICLES § 1288.6 Forms and reports. (a) DLA form 1454 will be prepared...

32 CFR 1288.6 - Forms and reports.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 6 2014-07-01 2014-07-01 false Forms and reports. 1288.6 Section 1288.6 National Defense Other Regulations Relating to National Defense DEFENSE LOGISTICS AGENCY MISCELLANEOUS REGISTRATION OF PRIVATELY OWNED MOTOR VEHICLES § 1288.6 Forms and reports. (a) DLA form 1454 will be prepared...

32 CFR 1288.6 - Forms and reports.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 6 2012-07-01 2012-07-01 false Forms and reports. 1288.6 Section 1288.6 National Defense Other Regulations Relating to National Defense DEFENSE LOGISTICS AGENCY MISCELLANEOUS REGISTRATION OF PRIVATELY OWNED MOTOR VEHICLES § 1288.6 Forms and reports. (a) DLA form 1454 will be prepared...

32 CFR 1288.6 - Forms and reports.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 6 2013-07-01 2013-07-01 false Forms and reports. 1288.6 Section 1288.6 National Defense Other Regulations Relating to National Defense DEFENSE LOGISTICS AGENCY MISCELLANEOUS REGISTRATION OF PRIVATELY OWNED MOTOR VEHICLES § 1288.6 Forms and reports. (a) DLA form 1454 will be prepared...

32 CFR 1288.6 - Forms and reports.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 6 2011-07-01 2011-07-01 false Forms and reports. 1288.6 Section 1288.6 National Defense Other Regulations Relating to National Defense DEFENSE LOGISTICS AGENCY MISCELLANEOUS REGISTRATION OF PRIVATELY OWNED MOTOR VEHICLES § 1288.6 Forms and reports. (a) DLA form 1454 will be prepared...

Phosphatidylazidothymidine and phosphatidyl-ddC: assessment of uptake in mouse lymphoid tissues and antiviral activities in human immunodeficiency virus-infected cells and in Rauscher leukemia virus-infected mice.

PubMed Central

Hostetler, K Y; Richman, D D; Sridhar, C N; Felgner, P L; Felgner, J; Ricci, J; Gardner, M F; Selleseth, D W; Ellis, M N

1994-01-01

During the early stages of human immunodeficiency virus (HIV) infection, although symptoms are absent and viral replication in peripheral blood mononuclear cells is low, substantial levels of HIV replication can be documented in lymphoid tissue [G. Pantaleo, C. Graziosi, J.F. Demarest, L. Butini, M. Montroni, C.H. Fox, J.M. Orenstein, D.P. Kotler, and A.S. Fauci, Nature (London) 362:355-358, 1993, and J. Embretsen, M. Zupancic, J.L. Ribas, A. Burke, P. Racz, K. Tenner-Tacz, and A.T. Haase, Nature (London) 362:359-362, 1993]. This observation suggests that earlier treatment of HIV infection may be indicated and that strategies for enhancing drug targeting to the lymphoid tissue reservoris of HIV infection may be beneficial. To address this issue, we synthesized dioleoylphosphatidyl-ddC (DOP-ddC) and dipalmitoylphosphatidyl-3'-azido-3'-deoxythymidine (DPP-AZT), phospholipid prodrugs which form lipid bilayers and which are readily incorporated into liposomes. The anti-HIV activity of DOP-ddC was similar to that of ddC in HIV type 1-infected HT4-6C cells, but DPP-AZT was considerably less active than AZT in HT4-6C cells. Liposomes containing DOP-[3H]ddC or DPP-[3H]AZT administered intraperitoneally to mice produced greater levels of total radioactivity over time in plasma, spleen, and lymphoid tissue relative to the results with [3H]ddC and [3H]AZT, respectively. DPP-AZT administered intraperitoneally in liposomes as a single daily dose to mice infected with Rauscher leukemia virus prevented increased spleen weight and reverse transcriptase levels in serum with a dose-response roughly comparable to that of AZT given continuously in the drinking water. DOP-ddC, DPP-AZT, and lipid conjugates of other antiretroviral nucleosides may provide higher levels of drug over time in plasma and in lymph nodes and spleen, important reservoirs of HIV infection, and may represent an interesting alternative approach to antiviral nucleoside treatment of AIDS. PMID:7695264

CTA1-DD adjuvant promotes strong immunity against human immunodeficiency virus type 1 envelope glycoproteins following mucosal immunization.

PubMed

Sundling, Christopher; Schön, Karin; Mörner, Andreas; Forsell, Mattias N E; Wyatt, Richard T; Thorstensson, Rigmor; Karlsson Hedestam, Gunilla B; Lycke, Nils Y

2008-12-01

Strategies to induce potent and broad antibody responses against the human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins (Env) at both systemic and mucosal sites represent a central goal for HIV-1 vaccine development. Here, we show that the non-toxic CTA1-DD adjuvant promoted mucosal and systemic humoral and cell-mediated immune responses following intranasal (i.n.) immunizations with trimeric or monomeric forms of HIV-1 Env in mice and in non-human primates. Env-specific IgG subclasses in the serum of immunized mice reflected a balanced Th1/Th2 type of response. Strikingly, i.n. immunizations with Env and the CTA1-DD adjuvant induced substantial levels of mucosal anti-Env IgA in bronchial alveolar lavage and also detectable levels in vaginal secretions. By contrast, parenteral immunizations of Env formulated in Ribi did not stimulate mucosal IgA responses, while the two adjuvants induced a similar distribution of Env-specific IgG-subclasses in serum. A single parenteral boost with Env in Ribi adjuvant into mice previously primed i.n. with Env and CTA1-DD, augmented the serum anti-Env IgG levels to similar magnitudes as those observed after three intraperitoneal immunizations with Env in Ribi. The augmenting potency of CTA1-DD was similar to that of LTK63 or CpG oligodeoxynucleotides (ODN). However, in contrast to CpG ODN, the effect of CTA1-DD and LTK63 appeared to be independent of MyD88 and toll-like receptor signalling. This is the first demonstration that CTA1-DD augments specific immune responses also in non-human primates, suggesting that this adjuvant could be explored further as a clinically safe mucosal vaccine adjuvant for humoral and cell-mediated immunity against HIV-1 Env.

Partial genetic suppression of a loss-of-function mutant of the neuronal ceroid lipofuscinosis-associated protease TPP1 in Dictyostelium discoideum

PubMed Central

Phillips, Jonathan E.; Gomer, Richard H.

2015-01-01

Neuronal ceroid lipofuscinosis (NCL) is the most common childhood-onset neurodegenerative disease. NCL is inevitably fatal, and there is currently no treatment available. Children with NCL show a progressive decline in movement, vision and mental abilities, and an accumulation of autofluorescent deposits in neurons and other cell types. Late-infantile NCL is caused by mutations in the lysosomal protease tripeptidyl peptidase 1 (TPP1). TPP1 cleaves tripeptides from the N-terminus of proteins in vitro, but little is known about the physiological function of TPP1. TPP1 shows wide conservation in vertebrates but it is not found in Drosophila, Caenorhabditis elegans or Saccharomyces cerevisiae. Here, we characterize ddTpp1, a TPP1 ortholog present in the social amoeba Dictyostelium discoideum. Lysates from cells lacking ddTpp1 show a reduced but not abolished ability to cleave a TPP1 substrate, suggesting that other Dictyostelium enzymes can perform this cleavage. ddTpp1 and human TPP1 localize to the lysosome in Dictyostelium, indicating conserved function and trafficking. Cells that lack ddTpp1 show precocious multicellular development and a reduced ability to form spores during development. When cultured in autophagy-stimulating conditions, cells lacking ddTpp1 rapidly decrease in size and are less viable than wild-type cells, suggesting that one function of ddTpp1 could be to limit autophagy. Cells that lack ddTpp1 exhibit strongly impaired development in the presence of the lysosome-perturbing drug chloroquine, and this phenotype can be suppressed through a secondary mutation in the gene that we name suppressor of tpp1− A (stpA), which encodes a protein with some similarity to mammalian oxysterol-binding proteins (OSBPs). Taken together, these results suggest that targeting specific proteins could be a viable way to suppress the effects of loss of TPP1 function. PMID:25540127

Neurocognitive accounts of developmental dyscalculia and its remediation.

PubMed

Iuculano, T

2016-01-01

Numbers are one of the most pervasive stimulus categories in our environment and an integral foundation of modern society. Yet, up to 20% of individuals fail to understand, represent, and manipulate numbers and form the basis of arithmetic, a condition termed developmental dyscalculia (DD). Multiple cognitive and neural systems including those that serve numerical, mnemonic, visuospatial, and cognitive control functions have independently been implicated in the etiology of DD, yet most studies have not taken a comprehensive or dynamic view of the disorder. This chapter supports the view of DD as a multifaceted neurodevelopmental disorder that is the result of multiple aberrancies at one or multiple levels of the information processing hierarchy, which supports successful arithmetic learning, and suggests that interventions should target all these systems to achieve successful outcomes, at the behavioral and neural levels. © 2016 Elsevier B.V. All rights reserved.

32 CFR 1290.9 - Forms and reports.

Code of Federal Regulations, 2014 CFR

2014-07-01

.... Magistrate system is based on use of a four-ply ticket designed to provide legal notice to violators and... departments. The DD Form 1805 is printed on chemically carbonized paper and prenumbered in series for... 635, Security/Criminal Incident Report, denoting the date, time, place, and type of violation, and the...

32 CFR 1290.9 - Forms and reports.

Code of Federal Regulations, 2013 CFR

2013-07-01

.... Magistrate system is based on use of a four-ply ticket designed to provide legal notice to violators and... departments. The DD Form 1805 is printed on chemically carbonized paper and prenumbered in series for... 635, Security/Criminal Incident Report, denoting the date, time, place, and type of violation, and the...

32 CFR 1290.9 - Forms and reports.

Code of Federal Regulations, 2012 CFR

2012-07-01

.... Magistrate system is based on use of a four-ply ticket designed to provide legal notice to violators and... departments. The DD Form 1805 is printed on chemically carbonized paper and prenumbered in series for... 635, Security/Criminal Incident Report, denoting the date, time, place, and type of violation, and the...

78 FR 31523 - Submission for OMB Review; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-24

... the student, school the student is attending, type of education benefits being used, branch of the..., Associated Form and OMB Number: Web-based DoD Postsecondary Education Complaint System, 0704-TBD; DoD Postsecondary Education Complaint Intake--DD Form 2961 (electronic-only). Type of Request: New Collection...

32 CFR 65.8 - Reporting requirements.

Code of Federal Regulations, 2010 CFR

2010-07-01

... assigned Report Control Symbols DD-P&R(AR)1221, DD-P&R(Q)2077, DD-RA(M)1147, DD-RA(D)1148, DD-RA(D)2170, DD-RA(M)2171, DD-RA(D)2302, and DD-RA(M)2303 in accordance with the requirements of DoD 8910.1-M...

A potential oncogenic activity of platelet-derived growth factor d in prostate cancer progression.

PubMed

Ustach, Carolyn V; Taube, Marcus E; Hurst, Newton J; Bhagat, Sunita; Bonfil, R Daniel; Cher, Michael L; Schuger, Lucia; Kim, Hyeong-Reh Choi

2004-03-01

The platelet-derived growth factor (PDGF) proteins are potent stimulators of cell proliferation/transformation and play a major role in cell-cell communication. For over two decades, PDGFs were thought to exist as three dimeric polypeptides (the homodimers AA and BB and the heterodimer AB). Recently, however, the PDGF C and D chains were discovered in a BLAST search of the expressed sequence tag databases. The PDGF CC and DD dimers have a unique two-domain structure with an NH(2)-terminal CUB (compliment subcomponents C1r/C1s, Uegf, and Bmp1) domain and a COOH-terminal PDGF/vascular endothelial growth factor domain. Whereas secreted PDGF AA, BB, and AB readily activate their cell surface receptors, it was suggested that extracellular proteolytic removal of the CUB domain is required for the PDGF/vascular endothelial growth factor domain of PDGF CC and DD to activate PDGF receptors. In the present study, we examined the processing of latent PDGF D into its active form and the effects of PDGF D expression on prostate cancer progression. We show that LNCaP cells auto-activate latent PDGF DD into the active PDGF domain, which can induce phosphorylation of the beta-PDGF receptor and stimulates LNCaP cell proliferation in an autocrine manner. Additionally, LNCaP-PDGF D-conditioned medium induces migration of the prostate fibroblast cell line 1532-FTX, indicating LNCaP-processed PDGF DD is active in a paracrine manner as well. In a severe combined immunodeficient mouse model, PDGF DD expression accelerates early onset of prostate tumor growth and drastically enhances prostate carcinoma cell interaction with surrounding stromal cells. These demonstrate a potential oncogenic activity of PDGF DD in the development and/or progression of prostate cancer.

A Potential Oncogenic Activity of Platelet-Derived Growth Factor D in Prostate Cancer Progression

PubMed Central

Ustach, Carolyn V.; Taube, Marcus E.; Hurst, Newton J.; Bhagat, Sunita; Bonfil, R. Daniel; Cher, Michael L.; Schuger, Lucia; Kim, Hyeong-Reh Choi

2014-01-01

The platelet-derived growth factor (PDGF) proteins are potent stimulators of cell proliferation/transformation and play a major role in cell-cell communication. For over two decades, PDGFs were thought to exist as three dimeric polypeptides (the homodimers AA and BB and the heterodimer AB). Recently, however, the PDGF C and D chains were discovered in a BLAST search of the expressed sequence tag databases. The PDGF CC and DD dimers have a unique two-domain structure with an NH2-terminal CUB (compliment subcomponents C1r/C1s, Uegf, and Bmp1) domain and a COOH-terminal PDGF/vascular endothelial growth factor domain. Whereas secreted PDGF AA, BB, and AB readily activate their cell surface receptors, it was suggested that extracellular proteolytic removal of the CUB domain is required for the PDGF/vascular endothelial growth factor domain of PDGF CC and DD to activate PDGF receptors. In the present study, we examined the processing of latent PDGF D into its active form and the effects of PDGF D expression on prostate cancer progression. We show that LNCaP cells auto-activate latent PDGF DD into the active PDGF domain, which can induce phosphorylation of the β-PDGF receptor and stimulates LNCaP cell proliferation in an autocrine manner. Additionally, LNCaP-PDGF D-conditioned medium induces migration of the prostate fibroblast cell line 1532-FTX, indicating LNCaP-processed PDGF DD is active in a paracrine manner as well. In a severe combined immunodeficient mouse model, PDGF DD expression accelerates early onset of prostate tumor growth and drastically enhances prostate carcinoma cell interaction with surrounding stromal cells. These demonstrate a potential oncogenic activity of PDGF DD in the development and/or progression of prostate cancer. PMID:14996732

The Redundancy of Peptidoglycan Carboxypeptidases Ensures Robust Cell Shape Maintenance in Escherichia coli

PubMed Central

Peters, Katharina; Kannan, Suresh; Rao, Vincenzo A.; Biboy, Jacob; Vollmer, Daniela; Erickson, Stephen W.; Lewis, Richard J.

2016-01-01

ABSTRACT Peptidoglycan (PG) is an essential structural component of the bacterial cell wall and maintains the integrity and shape of the cell by forming a continuous layer around the cytoplasmic membrane. The thin PG layer of Escherichia coli resides in the periplasm, a unique compartment whose composition and pH can vary depending on the local environment of the cell. Hence, the growth of the PG layer must be sufficiently robust to allow cell growth and division under different conditions. We have analyzed the PG composition of 28 mutants lacking multiple PG enzymes (penicillin-binding proteins [PBPs]) after growth in acidic or near-neutral-pH media. Statistical analysis of the muropeptide profiles identified dd-carboxypeptidases (DD-CPases) that were more active in cells grown at acidic pH. In particular, the absence of the DD-CPase PBP6b caused a significant increase in the pentapeptide content of PG as well as morphological defects when the cells were grown at acidic pH. Other DD-CPases (PBP4, PBP4b, PBP5, PBP6a, PBP7, and AmpH) and the PG synthase PBP1B made a smaller or null contribution to the pentapeptide-trimming activity at acidic pH. We solved the crystal structure of PBP6b and also demonstrated that the enzyme is more stable and has a lower Km at acidic pH, explaining why PBP6b is more active at low pH. Hence, PBP6b is a specialized DD-CPase that contributes to cell shape maintenance at low pH, and E. coli appears to utilize redundant DD-CPases for normal growth under different conditions. PMID:27329754

Numerical calculations of energy, nucleus size and coulomb decay rate for ddμ* resonance states in the variational approach using new wavefunctions

NASA Astrophysics Data System (ADS)

Eskandari, M. R.; Gheisari, R.; Kashian, S.

2006-02-01

This paper provides a theoretical complement to the experimental measurement of the population of excited dμ(2s) and dμ(1s) atoms in a deuterium. The population of these atoms plays an important role in a muon catalyzed fusion cycle. Symmetric and non-symmetric muonic molecular ions have been predicted to form in excited states in collisions between excited muonic atoms and hydrogen molecules. One example is the ddμ*, which is a muonic deuterium-deuterium symmetric ion in excited state and is initially produced in the interaction of dμ(2s) atoms with deuterium nuclei. Our calculations interpret the experimental findings in terms of the so-called side-path model. This model essentially deals with the interaction mentioned above in which the ddμ* ion undergoes Coulomb de-excitation where the excitation energy is shared between a dμ(1s) atom and one deuterium. The structure of ddμ* is studied here using the numerical, variational method and the given wavefunctions. Few resonance energies for ddμ* molecular states are calculated below the 2s threshold. For more precise assessment of the reliability of the given wavefunctions, the nucleus sizes and Coulomb decay rates for the zeroth, first and second vibrational meta-stable states of the mentioned ion are also calculated. The obtained results are close to those previously reported. The advantage of the given method over previous methods is that the used wavefunction has only two terms, which simplifies the calculations with the same results as those from the complicated coupled rearrangement channel method with a Gaussian basis set. These energies are the base data required for size, formation and decay rate calculations of the ddμ* ion.

Factors influencing work participation of adults with developmental dyslexia: a systematic review.

PubMed

de Beer, Joost; Engels, Josephine; Heerkens, Yvonne; van der Klink, Jac

2014-01-24

Evidence has been synthesized to determine hindering and facilitating factors associated with the work participation of adults with developmental dyslexia (DD), classified according to the International Classification of Functioning, Disability and Health (ICF). A systematic literature review has been performed. Two search strings were used to determine the population and the context of work. The ICF was expanded with two subdivisions: one that made the environmental factors more work-related and a subdivision of personal factors. For data extraction the method known as qualitative metasummary was used and the manifest frequency effect size (MFES) for each category in the ICF was calculated. From 33 included studies 318 factors have been extracted and classified in the ICF. In the classification the frequency of occurrences and the consistency in direction (i.e., hindering or facilitating) have been made visible. The ICF categories with the highest MFES were mental functions with factors like feelings and emotions about dyslexia; activities like reading or writing/spelling; participation with factors like acquiring and keeping a job; social relationships at work where the attitudes and support of the employer and co-workers are important; working conditions with factors like the availability of assistive technology and accommodations on the job; and personal factors like self-disclosure and coping strategies. In the context of work DD affects nearly all domains of functioning, mostly in a negative way. Within each domain the impact of DD increases over the course of life. To overcome that negative influence, many forms of adaptation, compensation, or coping are mentioned. Also notable is the lack of positive attitudes toward DD of the participants with DD-with the exception of the attitudes of teachers with DD-as well as on the part of colleagues, supervisors, and employers.

Mixed anxiety and depressive disorder before and after psychodynamic group psychotherapy: a 1-year follow-up study.

PubMed

Małyszczak, Krzysztof; Frydecka, Dorota; Pawłowski, Tomasz; Kiejna, Andrzej

2010-11-01

Abstract Objective. The aim of our study was to observe the outcome of MADD in comparison with depressive (DD) and anxiety (AD) disorders. Method. Patients treated with 12 weeks of group psychodynamic psychotherapy in a psychiatric day care ward were examined using SCAN 2.1 at admission and 1 year after admission. Treatment was indicated on the basis of diagnosis of ICD-10 - F4-F6. A total of 139 patients were included, 110 (79.1%) of whom were examined at the follow-up point. Results. The prevalence of MADD increased from 22.7% at the baseline to 33.6% at the end. The outcome of MADD was statistically different from the outcome of DD (χ(2)=18.4, P=0.0025), but not different from the outcome of comorbid DD and AD (χ(2)=1.8, P=0.84), nor generalized anxiety disorder (χ(2)=8.1, P=0.15), nor other AD (χ(2)=5.3, P=0.38). Conclusion. MADD is a useful diagnosis of a transitional or residual form of comorbid DD and AD in some specific population groups. A diagnosis of personality disorder can sustain long-term diagnosis of MADD.

48 CFR 52.204-2 - Security Requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Agreement (DD Form 441), including the National Industrial Security Program Operating Manual (DOD 5220.22-M... architect-engineer contract, add the following paragraph (e) to the basic clause: (e) The Contractor shall...

32 CFR 1290.7 - Responsibilities.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Responsibilities. (a) HQ DLA. (1) The Command Security Officer, DLA (DLA-T) will: (i) Exercise staff supervision... enforcement/security force (080, 083, 085 and 1800 series) personnel to issue DD Form 1805. (3) Periodically...

32 CFR 1290.7 - Responsibilities.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Responsibilities. (a) HQ DLA. (1) The Command Security Officer, DLA (DLA-T) will: (i) Exercise staff supervision... enforcement/security force (080, 083, 085 and 1800 series) personnel to issue DD Form 1805. (3) Periodically...

78 FR 58525 - Proposed Collection; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-24

... Assistance; DD form 1607; OMB Control Number 0704-0463. Needs and Uses: The Department plans to expand its... 1607, ``Application for Homeowners Assistance'' to apply. The application is reviewed by a department...

Defense programs beryllium good practice guide

DOE Office of Scientific and Technical Information (OSTI.GOV)

Herr, M.

1997-07-01

Within the DOE, it has recently become apparent that some contractor employees who have worked (or are currently working) with and around beryllium have developed chronic beryllium disease (CBD), an occupational granulomatous lung disorder. Respiratory exposure to aerosolized beryllium, in susceptible individuals, causes an immunological reaction that can result in granulomatous scarring of the lung parenchyma, shortness of breath, cough, fatigue, weight loss, and, ultimately, respiratory failure. Beryllium disease was originally identified in the 1940s, largely in the fluorescent light industry. In 1950, the Atomic Energy Commission (AEC) introduced strict exposure standards that generally curtailed both the acute and chronicmore » forms of the disease. Beginning in 1984, with the identification of a CBD case in a DOE contractor worker, there was increased scrutiny of both industrial hygiene practices and individuals in this workforce. To date, over 100 additional cases of beryllium-specific sensitization and/or CBD have been identified. Thus, a disease previously thought to be largely eliminated by the adoption of permissible exposure standards 45 years ago is still a health risk in certain workforces. This good practice guide forms the basis of an acceptable program for controlling workplace exposure to beryllium. It provides (1) Guidance for minimizing worker exposure to beryllium in Defense Programs facilities during all phases of beryllium-related work, including the decontamination and decommissioning (D&D) of facilities. (2) Recommended controls to be applied to the handling of metallic beryllium and beryllium alloys, beryllium oxide, and other beryllium compounds. (3) Recommendations for medical monitoring and surveillance of workers exposed (or potentially exposed) to beryllium, based on the best current understanding of beryllium disease and medical diagnostic tests available. (4) Site-specific safety procedures for all processes of beryllium that is likely to generate dusts, mists, fumes, or small particulates. A beryllium exposure control program should minimize airborne concentrations, the potential for and spread of contamination, the number of times individuals are exposed to beryllium, and the number of employees who may be potentially exposed.« less

32 CFR 634.20 - Privately owned vehicle operation requirements.

Code of Federal Regulations, 2012 CFR

2012-07-01

... nation or international drivers license (within appropriate classification), supported by DD Form 2, or... Safety Administration (NHTSA) in 49 CFR 570.1 through 570.10. Lights, turn signals, brake lights, horn...

32 CFR 634.20 - Privately owned vehicle operation requirements.

Code of Federal Regulations, 2014 CFR

2014-07-01

... nation or international drivers license (within appropriate classification), supported by DD Form 2, or... Safety Administration (NHTSA) in 49 CFR 570.1 through 570.10. Lights, turn signals, brake lights, horn...

32 CFR 634.20 - Privately owned vehicle operation requirements.

Code of Federal Regulations, 2013 CFR

2013-07-01

... nation or international drivers license (within appropriate classification), supported by DD Form 2, or... Safety Administration (NHTSA) in 49 CFR 570.1 through 570.10. Lights, turn signals, brake lights, horn...

Preliminary Results from Pyroelectric Crystal Accelerator

DOE Office of Scientific and Technical Information (OSTI.GOV)

Anderson, Tom; Edwards, Ronald; Bright, Kevin

The Nuclear Science and Engineering Research Center (NSERC), a Defense Threat Reduction Agency (DTRA) office located at the United States Military Academy (USMA), sponsors and manages cadet and faculty research in support of DTRA objectives. Cadets in the Department of Physics and Nuclear Engineering at USMA are using pyroelectric crystals to ionize and accelerate residual gas trapped inside a vacuum system. A system using two lithium tantalate crystals with associated diagnostics was designed and is now operational. X-ray energies of approximately 150 keV have been achieved. Future work will focus on developing a portable neutron generator using the D-D nuclearmore » fusion process.« less

Modernization of Defense Logistics Standard Systems. Establishing the Functional Baseline. Volume 2. Appendix H

DTIC Science & Technology

1991-09-01

and "DD" is the numeric value of the day (01-31). [61 Time ( TM ). The time type is symbolized by the representation " TM ." Format for this type is...VERSION ID M DT 06/06 M TM 04/04 M ID 01/01 M ID 05/05 ISA13 112 ISA14 113 ISA15 114 ISA16 115INTERCHANGE *ACKNOWLEDG. * S1TEST 14 * SUBELEMENT N CTRL...Max - 6 Date of the interchange. DLMS Usage: As above. 109 Interchange Time Type - TM Min - 4 Max - 4 Time of the interchange. DLMS Usage: As above

Somatic GNAQ mutation in the forme fruste of Sturge-Weber syndrome

PubMed Central

Harvey, A. Simon; Malone, Stephen; Damiano, John A.; Do, Hongdo; Ye, Zimeng; McQuillan, Lara; Maixner, Wirginia; Kalnins, Renate; Nolan, Bernadette; Wood, Martin; Ozturk, Ezgi; Jones, Nigel C.; Gillies, Greta; Pope, Kate; Lockhart, Paul J.; Dobrovic, Alexander; Leventer, Richard J.; Scheffer, Ingrid E.

2018-01-01

Objective To determine whether the GNAQ R183Q mutation is present in the forme fruste cases of Sturge-Weber syndrome (SWS) to establish a definitive molecular diagnosis. Methods We used sensitive droplet digital PCR (ddPCR) to detect and quantify the GNAQ mutation in tissues from epilepsy surgery in 4 patients with leptomeningeal angiomatosis; none had ocular or cutaneous manifestations. Results Low levels of the GNAQ mutation were detected in the brain tissue of all 4 cases—ranging from 0.42% to 7.1% frequency—but not in blood-derived DNA. Molecular evaluation confirmed the diagnosis in 1 case in which the radiologic and pathologic data were equivocal. Conclusions We detected the mutation at low levels, consistent with mosaicism in the brain or skin (1.0%–18.1%) of classic cases. Our data confirm that the forme fruste is part of the spectrum of SWS, with the same molecular mechanism as the classic disease and that ddPCR is helpful where conventional diagnosis is uncertain. PMID:29725622

Atmospheric Renewable Energy Research, Volume 5 (Solar Radiation Flux Model)

DTIC Science & Technology

2017-09-01

Approved for public release; distribution is unlimited. ii REPORT DOCUMENTATION PAGE Form Approved OMB No... FORM TO THE ABOVE ADDRESS. 1. REPORT DATE (DD-MM-YYYY) September 2017 2. REPORT TYPE Technical Report 3. DATES COVERED (From - To) 2017 June 01...3237 Standard Form 298 (Rev. 8/98) Prescribed by ANSI Std. Z39.18 Approved for public release; distribution is unlimited. iii Contents List

12 CFR Appendix B to Part 230 - Model Clauses and Sample Forms

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Model Clauses and Sample Forms B Appendix B to... SYSTEM TRUTH IN SAVINGS (REGULATION DD) Pt. 230, App. B Appendix B to Part 230—Model Clauses and Sample Forms Table of contents B-1—Model Clauses for Account Disclosures (Section 230.4(b)) B-2—Model Clauses...

Growth and Energy Demand of Meloidogyne incognita on Susceptible and Resistant Vitis vinifera Cultivars.

PubMed

Melakeberhan, H; Ferris, H

1988-10-01

Food (energy) consumption rates ofMeloidogyne incognita were calculated on Vitis vinifera cv. French Colombard (highly susceptible) and cv. Thompson Seedless (moderately resistant). One-month-old grape seedlings in styrofoam cups were inoculated with 2,000 or 8,000 M. incognita second-stage juveniles (J2) and maintained at 17.5 degree days (DD - base 10 C)/day until maximum adult female growth and (or) the end of oviposition. At 70 DD intervals, nematode fresh biomass was calculated on the basis of volumes of 15-20 nematodes per plant obtained with a digitizer and computer algorithm. Egg production was measured at 50-80 DD intervals by weighing 7-10 egg masses and counting the number of eggs. Nematode growth and food (energy) consumption rates were calculated up to 1,000 DD based on biomass increase, respiratory requirements, and an assumption of 60 % assimilation efficiency. The growth rate of a single root-knot nematode, excluding egg production, was similar in both cultivars and had a logistic form. The maximum fresh weight of a mature female nematode was ca. 29-32 mug. The total biomass increase, including egg production, also had a logistic form. Maximum biomass (mature adult female and egg mass) was 211 mug on French Colombard and 127 mug on Thompson Seedless. The calculated total cost to the host for the development of a single J2 from root penetration to the end of oviposition for body growth and total biomass was 0.535 and 0.486 calories with a total energy demand of 1.176 and 0.834 calories in French Colombard and Thompson Seedless, respectively.

Substrate mimicry: HIV-1 reverse transcriptase recognizes 6-modified-3′-azido-2′,3′-dideoxyguanosine-5′-triphosphates as adenosine analogs

PubMed Central

Herman, Brian D.; Schinazi, Raymond F.; Zhang, Hong-wang; Nettles, James H.; Stanton, Richard; Detorio, Mervi; Obikhod, Aleksandr; Pradère, Ugo; Coats, Steven J.; Mellors, John W.; Sluis-Cremer, Nicolas

2012-01-01

β-D-3′-Azido-2′,3′-dideoxyguanosine (3′-azido-ddG) is a potent inhibitor of HIV-1 replication with a superior resistance profile to zidovudine. Recently, we identified five novel 6-modified-3′-azido-ddG analogs that exhibit similar or superior anti-HIV-1 activity compared to 3′-azido-ddG in primary cells. To gain insight into their structure–activity–resistance relationships, we synthesized their triphosphate (TP) forms and assessed their ability to inhibit HIV-1 reverse transcriptase (RT). Steady-state and pre-steady-state kinetic experiments show that the 6-modified-3′-azido-ddGTP analogs act as adenosine rather than guanosine mimetics in DNA synthesis reactions. The order of potency of the TP analogs against wild-type RT was: 3′-azido-2,6-diaminopurine >3′-azido-6-chloropurine; 3′-azido-6-N-allylaminopurine > 2-amino-6-N,N-dimethylaminopurine; 2-amino-6-methoxypurine. Molecular modeling studies reveal unique hydrogen-bonding interactions between the nucleotide analogs and the template thymine base in the active site of RT. Surprisingly, the structure–activity relationship of the analogs differed in HIV-1 RT ATP-mediated excision assays of their monophosphate forms, suggesting that it may be possible to rationally design a modified base analog that is efficiently incorporated by RT but serves as a poor substrate for ATP-mediated excision reactions. Overall, these studies identify a promising strategy to design novel nucleoside analogs that exert profound antiviral activity against both WT and drug-resistant HIV-1. PMID:21914723

32 CFR 855.8 - Application procedures.

Code of Federal Regulations, 2010 CFR

2010-07-01

.... To allow time for processing, the application (DD Forms 2400, 2401, and 2402) and a self-addressed... verification required for each purpose of use must be included with the application. The name of the user must...

75 FR 60422 - Submission for OMB Review; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-30

... November 1, 2010. Title and OMB Number: Trustee Report, DD Form 2826, OMB Number 0730-0012. Type of Request... fraud, waste and abuse of Government funds and member's benefits. Affected Public: Individuals or...

Using Elemental Literary Forms in the Composition Class

ERIC Educational Resources Information Center

Harp, Richard L.

1978-01-01

Concludes that imaginative literature is the best way to begin to teach writing and the literature to teach first should be the most basic and elemental types: fable, fairy tale, parable, proverb, and myth. (DD)

32 CFR 761.19 - Forms.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 5 2010-07-01 2010-07-01 false Forms. 761.19 Section 761.19 National Defense Department of Defense (Continued) DEPARTMENT OF THE NAVY ISLANDS UNDER NAVY JURISDICTION NAVAL DEFENSIVE SEA... the processing of applications for authorization to enter defense areas and for revocation of...

32 CFR 761.19 - Forms.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 5 2012-07-01 2012-07-01 false Forms. 761.19 Section 761.19 National Defense Department of Defense (Continued) DEPARTMENT OF THE NAVY ISLANDS UNDER NAVY JURISDICTION NAVAL DEFENSIVE SEA... the processing of applications for authorization to enter defense areas and for revocation of...

32 CFR 761.19 - Forms.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 5 2011-07-01 2011-07-01 false Forms. 761.19 Section 761.19 National Defense Department of Defense (Continued) DEPARTMENT OF THE NAVY ISLANDS UNDER NAVY JURISDICTION NAVAL DEFENSIVE SEA... the processing of applications for authorization to enter defense areas and for revocation of...

32 CFR 761.19 - Forms.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 5 2014-07-01 2014-07-01 false Forms. 761.19 Section 761.19 National Defense Department of Defense (Continued) DEPARTMENT OF THE NAVY ISLANDS UNDER NAVY JURISDICTION NAVAL DEFENSIVE SEA... the processing of applications for authorization to enter defense areas and for revocation of...

32 CFR 761.19 - Forms.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 5 2013-07-01 2013-07-01 false Forms. 761.19 Section 761.19 National Defense Department of Defense (Continued) DEPARTMENT OF THE NAVY ISLANDS UNDER NAVY JURISDICTION NAVAL DEFENSIVE SEA... the processing of applications for authorization to enter defense areas and for revocation of...

Oily nanosuspension for long-acting intramuscular delivery of curcumin didecanoate prodrug: preparation, characterization and in vivo evaluation.

PubMed

Wei, Xiao-Lan; Han, Ying-Rui; Quan, Li-Hui; Liu, Chun-Yu; Liao, Yong-Hong

2013-05-13

The objective of this study was to prepare the nanocrystals of curcumin didecanoate (CurDD) by wet ball milling and to investigate the comparative pharmacokinetics of oily nano- and micro-suspensions after intramuscular (i.m.) administration to rats. Upon optimizing the wet ball milling parameters, CurDD nanocrystals were produced with median particle size of ~500 nm and the freeze-dried nanocrystals were readily dispersed in peanut oil to form stable nanosuspensions. Although the nanosuspension appeared to exhibit slower clearance from the injection site after i.m. injection, compared to microsuspension (~5 μm), a significantly higher maximum plasma curcumin concentration (69.0 ng/ml) was observed for the former than that for the latter (18.5 ng/ml). In addition, the nanosuspension provided significant higher plasma curcumin concentrations and brain CurDD contents for at least 15 days than the microsuspension, except for the initial times. A single i.m. injection of nanosuspension appeared to achieve reversal effect on reserpine-induced hypothermia for at least 13 days. This study demonstrates that CurDD nanosuspension may act as a long-acting i.m. injectable for sustained delivery of curcumin, potentially applicable to elicit a long-lasting antidepressant effect. Copyright © 2013 Elsevier B.V. All rights reserved.

Genetic requirements for sensitivity of bacteriophage t7 to dideoxythymidine.

PubMed

Tran, Ngoc Q; Tabor, Stanley; Richardson, Charles C

2014-08-01

We previously reported that the presence of dideoxythymidine (ddT) in the growth medium selectively inhibits the ability of bacteriophage T7 to infect Escherichia coli by inhibiting phage DNA synthese (N. Q. Tran, L. F. Rezende, U. Qimron, C. C. Richardson, and S. Tabor, Proc. Natl. Acad. Sci. U. S. A. 105:9373-9378, 2008, doi:10.1073/pnas.0804164105). In the presence of T7 gene 1.7 protein, ddT is taken up into the E. coli cell and converted to ddTTP. ddTTP is incorporated into DNA as ddTMP by the T7 DNA polymerase, resulting in chain termination. We have identified the pathway by which exogenous ddT is converted to ddTTP. The pathway consists of ddT transport by host nucleoside permeases and phosphorylation to ddTMP by the host thymidine kinase. T7 gene 1.7 protein phosphorylates ddTMP and ddTDP, resulting in ddTTP. A 74-residue peptide of the gene 1.7 protein confers ddT sensitivity to the same extent as the 196-residue wild-type gene 1.7 protein. We also show that cleavage of thymidine to thymine and deoxyribose-1-phosphate by the host thymidine phosphorylase greatly increases the sensitivity of phage T7 to ddT. Finally, a mutation in T7 DNA polymerase that leads to discrimination against the incorporation of ddTMP eliminates ddT sensitivity. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

Genetic Requirements for Sensitivity of Bacteriophage T7 to Dideoxythymidine

PubMed Central

Tran, Ngoc Q.; Tabor, Stanley

2014-01-01

We previously reported that the presence of dideoxythymidine (ddT) in the growth medium selectively inhibits the ability of bacteriophage T7 to infect Escherichia coli by inhibiting phage DNA synthese (N. Q. Tran, L. F. Rezende, U. Qimron, C. C. Richardson, and S. Tabor, Proc. Natl. Acad. Sci. U. S. A. 105:9373–9378, 2008, doi:10.1073/pnas.0804164105). In the presence of T7 gene 1.7 protein, ddT is taken up into the E. coli cell and converted to ddTTP. ddTTP is incorporated into DNA as ddTMP by the T7 DNA polymerase, resulting in chain termination. We have identified the pathway by which exogenous ddT is converted to ddTTP. The pathway consists of ddT transport by host nucleoside permeases and phosphorylation to ddTMP by the host thymidine kinase. T7 gene 1.7 protein phosphorylates ddTMP and ddTDP, resulting in ddTTP. A 74-residue peptide of the gene 1.7 protein confers ddT sensitivity to the same extent as the 196-residue wild-type gene 1.7 protein. We also show that cleavage of thymidine to thymine and deoxyribose-1-phosphate by the host thymidine phosphorylase greatly increases the sensitivity of phage T7 to ddT. Finally, a mutation in T7 DNA polymerase that leads to discrimination against the incorporation of ddTMP eliminates ddT sensitivity. PMID:24858186

Assessing the quality of democratic deliberation: A case study of public deliberation on the ethics of surrogate consent for research

PubMed Central

De Vries, Raymond; Stanczyk, Aimee; Wall, Ian F.; Uhlmann, Rebecca; Damschroder, Laura J; Kim, Scott Y

2010-01-01

“Deliberative democracy” is an increasingly popular method for soliciting public input on health care policies. There are a number of ways of organizing deliberative democracy (DD) sessions, but they generally involve gathering a group of citizens, supplying them with information relevant to the policy in question, giving them time to interact with each other and with experts in the policy area, and collecting their informed and considered opinions. As the method has become more widely used, some have questioned the quality of the public input it generates. Although theorists of DD agree that “good” input – i.e., input that is the product of careful and thorough reflection – is an essential aspect of useful and effective deliberation, few have actually measured the quality of deliberative sessions. As part of a DD project organized to help guide policies on the morally complex question of allowing surrogate permission to enroll persons with dementia in medical research, we developed and tested measures of “quality of deliberation.” After a brief discussion of the substantive results of our research – survey data from participants in the DD sessions and control groups showed a significant change in participants' attitudes toward surrogate consent – we examine the process by which this change occurred, describing and assessing the characteristics of our DD sessions. We use both quantitative and qualitative data from our DD sessions, conducted in southeastern Michigan, United States, to examine four dimensions of the quality of deliberation: 1) equal participation by all members of the session, 2) respect for the opinions of others, 3) a willingness to adopt a societal perspective on the issue in question (rather than a focus on what is best for participants as individuals), and 4) reasoned justification of one's positions. We demonstrate that DD can be reliably used to elicit opinions of the public and show how analysis of the quality of deliberations can offer insight into the ways opinions about ethical dilemmas are formed and changed. PMID:20378225

Assessing the quality of democratic deliberation: a case study of public deliberation on the ethics of surrogate consent for research.

PubMed

De Vries, Raymond; Stanczyk, Aimee; Wall, Ian F; Uhlmann, Rebecca; Damschroder, Laura J; Kim, Scott Y

2010-06-01

"Deliberative democracy" is an increasingly popular method for soliciting public input on health care policies. There are a number of ways of organizing deliberative democracy (DD) sessions, but they generally involve gathering a group of citizens, supplying them with information relevant to the policy in question, giving them time to interact with each other and with experts in the policy area, and collecting their informed and considered opinions. As the method has become more widely used, some have questioned the quality of the public input it generates. Although theorists of DD agree that "good" input - i.e., input that is the product of careful and thorough reflection - is an essential aspect of useful and effective deliberation, few have actually measured the quality of deliberative sessions. As part of a DD project organized to help guide policies on the morally complex question of allowing surrogate permission to enroll persons with dementia in medical research, we developed and tested measures of "quality of deliberation." After a brief discussion of the substantive results of our research - survey data from participants in the DD sessions and control groups showed a significant change in participants' attitudes toward surrogate consent - we examine the process by which this change occurred, describing and assessing the characteristics of our DD sessions. We use both quantitative and qualitative data from our DD sessions, conducted in southeastern Michigan, United States, to examine four dimensions of the quality of deliberation: 1) equal participation by all members of the session, 2) respect for the opinions of others, 3) a willingness to adopt a societal perspective on the issue in question (rather than a focus on what is best for participants as individuals), and 4) reasoned justification of one's positions. We demonstrate that DD can be reliably used to elicit opinions of the public and show how analysis of the quality of deliberations can offer insight into the ways opinions about ethical dilemmas are formed and changed. Copyright 2010 Elsevier Ltd. All rights reserved.

Evidence that the Dictyostelium STAT protein Dd-STATa plays a role in the differentiation of inner basal disc cells and identification of a promoter element essential for expression in these cells.

PubMed

Shimada, Nao; Maruo, Toshinari; Maeda, Mineko; Urushihara, Hideko; Kawata, Takefumi

2005-02-01

Dd-STATa, a Dictyostelium homolog of the metazoan STAT (signal transducers and activators of transcription) proteins, is necessary in the slug for correct entry into culmination. Dd-STATa-null mutant fails to culminate and its phenotype correlates with the loss of a funnel-shaped core region, the pstAB core region, which expresses both the ecmA and ecmB genes. To understand how the differentiation of pstAB core cells is regulated, we identified an EST that is expressed in the core cells of normal slugs but down-regulated in the Dd-STATa-null mutant. This EST, SSK348, encodes a close homolog of the Dictyostelium acetyl-CoA synthetase (ACS). A promoter fragment of the cognate gene, aslA (acetyl-CoA synthetase-like A), was fused to a lacZ reporter and the expression pattern determined. As expected from the behavior of the endogenous aslA gene, the aslA::lacZ fusion gene is not expressed in Dd-STATa-null slugs. In parental cells, the aslA promoter is first activated in the funnel-shaped core cells located at the slug anterior, the "pstAB core." During culmination, the pstAB core cells move down, through the prespore cells, to form the inner part of the basal disc. As the spore mass climbs the stalk, the aslA gene comes to be expressed in cells of the upper and lower cups, structures that cradle the spore head. Deletion and point mutation analyses of the promoter identified an AT-rich sequence that is necessary for expression in the pstAB core. This acts in combination with repressor regions that prevent ectopic aslA expression in the pre-stalk regions of slugs and the stalks of culminants. Thus, this study confirms that Dd-STATa is necessary for the differentiation of pstAB core cells, by showing that it is needed for the activation of the aslA gene. It also identifies aslA promoter elements that are likely to be regulated, directly or indirectly, by Dd-STATa.

Identification, characterization, and functional analysis of Tube and Pelle homologs in the mud crab Scylla paramamosain.

PubMed

Li, Xin-Cang; Zhang, Xiao-Wen; Zhou, Jun-Fang; Ma, Hong-Yu; Liu, Zhi-Dong; Zhu, Lei; Yao, Xiao-Juan; Li, Lin-Gui; Fang, Wen-Hong

2013-01-01

Tube and Pelle are essential components in Drosophila Toll signaling pathway. In this study, we characterized a pair of crustacean homologs of Tube and Pelle in Scylla paramamosain, namely, SpTube and SpPelle, and analyzed their immune functions. The full-length cDNA of SpTube had 2052 bp with a 1578 bp open reading frame (ORF) encoding a protein with 525 aa. A death domain (DD) and a kinase domain were predicted in the deduced protein. The full-length cDNA of SpPelle had 3825 bp with a 3420 bp ORF encoding a protein with 1140 aa. The protein contained a DD and a kinase domain. Two conserved repeat motifs previously called Tube repeat motifs present only in insect Tube or Tube-like sequences were found between these two domains. Alignments and structure predictions demonstrated that SpTubeDD and SpPelleDD significantly differed in sequence and 3D structure. Similar to TubeDD, SpTubeDD contained three common conserved residues (R, K, and R) on one surface that may mediate SpMyD88 binding and two common residues (A and A) on the other surface that may contribute to Pelle binding. By contrast, SpPelleDD lacked similar conservative residues. SpTube, insect Tube-like kinases, and human IRAK4 were found to be RD kinases with an RD dipeptide in the kinase domain. SpPelle, Pelle, insect Pelle-like kinases, and human IRAK1 were found to be non-RD kinases lacking an RD dipeptide. Both SpTube and SpPelle were highly expressed in hemocytes, gills, and hepatopancreas. Upon challenge, SpTube and SpPele were significantly increased in hemocytes by Gram-negative or Gram-positive bacteria, whereas only SpPelle was elevated by White Spot Syndrome Virus. The pull-down assay showed that SpTube can bind to both SpMyD88 and SpPelle. These results suggest that SpTube, SpPelle, and SpMyD88 may form a trimeric complex involved in the immunity of mud crabs against both Gram-negative and Gram-positive bacteria.

Identification, Characterization, and Functional Analysis of Tube and Pelle Homologs in the Mud Crab Scylla paramamosain

PubMed Central

Zhou, Jun-Fang; Ma, Hong-Yu; Liu, Zhi-Dong; Zhu, Lei; Yao, Xiao-Juan; Li, Lin-Gui; Fang, Wen-Hong

2013-01-01

Tube and Pelle are essential components in Drosophila Toll signaling pathway. In this study, we characterized a pair of crustacean homologs of Tube and Pelle in Scylla paramamosain, namely, SpTube and SpPelle, and analyzed their immune functions. The full-length cDNA of SpTube had 2052 bp with a 1578 bp open reading frame (ORF) encoding a protein with 525 aa. A death domain (DD) and a kinase domain were predicted in the deduced protein. The full-length cDNA of SpPelle had 3825 bp with a 3420 bp ORF encoding a protein with 1140 aa. The protein contained a DD and a kinase domain. Two conserved repeat motifs previously called Tube repeat motifs present only in insect Tube or Tube-like sequences were found between these two domains. Alignments and structure predictions demonstrated that SpTubeDD and SpPelleDD significantly differed in sequence and 3D structure. Similar to TubeDD, SpTubeDD contained three common conserved residues (R, K, and R) on one surface that may mediate SpMyD88 binding and two common residues (A and A) on the other surface that may contribute to Pelle binding. By contrast, SpPelleDD lacked similar conservative residues. SpTube, insect Tube-like kinases, and human IRAK4 were found to be RD kinases with an RD dipeptide in the kinase domain. SpPelle, Pelle, insect Pelle-like kinases, and human IRAK1 were found to be non-RD kinases lacking an RD dipeptide. Both SpTube and SpPelle were highly expressed in hemocytes, gills, and hepatopancreas. Upon challenge, SpTube and SpPele were significantly increased in hemocytes by Gram-negative or Gram-positive bacteria, whereas only SpPelle was elevated by White Spot Syndrome Virus. The pull-down assay showed that SpTube can bind to both SpMyD88 and SpPelle. These results suggest that SpTube, SpPelle, and SpMyD88 may form a trimeric complex involved in the immunity of mud crabs against both Gram-negative and Gram-positive bacteria. PMID:24116143

Structure of D-AKAP2:PKA RI complex: Insights into AKAP specificity and selectivity

PubMed Central

Sarma, Ganapathy N.; Kinderman, Francis S.; Kim, Choel; von Daake, Sventja; Chen, Lirong; Wang, Bi-Cheng; Taylor, Susan S.

2011-01-01

Summary A-kinase anchoring proteins (AKAPs) regulate cyclic AMP-dependent protein kinase (PKA) signaling in space and time. Dual-specific AKAP 2 (D-AKAP2) binds to the dimerization/docking (D/D) domain of both RI and RII regulatory subunits of PKA with high affinity. Here, we have determined the structures of the RIα D/D domain alone and in complex with D-AKAP2. The D/D domain presents an extensive surface for binding through a well-formed N-termina helix and this surface restricts the diversity of AKAPs that can interact. The structures also underscore the importance of a redox-sensitive disulfide in affecting AKAP binding. An unexpected shift in the helical register of D-AKAP2 compared to the RIIα:D-AKAP2 complex structure makes the mode of binding to RIα novel. Finally, the comparison allows us to deduce a molecular explanation for the sequence and spatial determinants of AKAP specificity. PMID:20159461

Fluorescent Lamp Replacement Study

DTIC Science & Technology

2017-07-01

DOCUMENTATION PAGE Form Approved OMB No. 0704-0188 The public reporting burden for this collection of information is estimated to average 1 hour per...information if it does not display a currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ORGANIZATION. 1. REPORT DATE (DD-MM...Unclassified 19b. TELEPHONE NUMBER (Include area code) Standard Form 298 (Rev. 8/98) Prescribed by ANSI Std. Z39.18 TABLE OF CONTENTS

Artificial Intelligence: A Revolution Waiting to Happen

DTIC Science & Technology

2017-06-09

REPORT DOCUMENTATION PAGE Form Approved OMB No. 0704-0188 Public reporting burden for this collection of information is estimated to average 1...currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. 1. REPORT DATE (DD-MM-YYYY) 9-06-2017 2. REPORT TYPE...important RMAs, limited by innovative concepts and organizational desire to change. General AI, on the other hand, looms in theoretical form as a

DNA EMP Awareness Course Notes. Supplement to Third Edition.

DTIC Science & Technology

1978-07-31

UNCLASSIFIED SECURITY CLASSIFICATION OF THIS PAGE (When Data Entered) REPORT DOCUMENTATION PAGE READ INSTRUCTIONS BEFORE COMPLETING FORM I REPORT...the environment through system design and testing. FORM 143 E,,N F’NOV 65 IS OBSOLETE DD JAN73 1473 EDITION OF UNCLASSIFIED SECURITY CLASSIFICATION OF...fields generated tems mission and deployment factors by the prompt gammas. Other forms of EMP, where these environments should be con- such as

A RabGAP Regulates Life-Cycle Duration via Trimeric G-protein Cascades in Dictyostelium discoideum

PubMed Central

Kuwayama, Hidekazu; Miyanaga, Yukihiro; Urushihara, Hideko; Ueda, Masahiro

2013-01-01

Background The life-cycle of cellular slime molds comprises chronobiologically regulated processes. During the growth phase, the amoeboid cells proliferate at a definite rate. Upon starvation, they synthesize cAMP as both first and second messengers in signalling pathways and form aggregates, migrating slugs, and fruiting bodies, consisting of spores and stalk cells, within 24 h. In Dictyostelium discoideum, because most growth-specific events cease during development, proliferative and heterochronic mutations are not considered to be interrelated and no genetic factor governing the entire life-cycle duration has ever been identified. Methodology/Principal Findings Using yeast 2-hybrid library screening, we isolated a Dictyostelium discoideum RabGAP, Dd Rbg-3, as a candidate molecule by which the Dictyostelium Gα2 subunit directs its effects. Rab GTPase-activating protein, RabGAP, acts as a negative regulator of Rab small GTPases, which orchestrate the intracellular membrane trafficking involved in cell proliferation. Deletion mutants of Dd rbg-3 exhibited an increased growth rate and a shortened developmental period, while an overexpression mutant demonstrated the opposite effects. We also show that Dd Rbg-3 interacts with 2 Gα subunits in an activity-dependent manner in vitro. Furthermore, both human and Caenorhabditis elegans rbg-3 homologs complemented the Dd rbg-3–deletion phenotype in D. discoideum, indicating that similar pathways may be generally conserved in multicellular organisms. Conclusions/Significance Our findings suggest that Dd Rbg-3 acts as a key element regulating the duration of D. discoideum life-span potentially via trimeric G-protein cascades. PMID:24349132

Trends in dual diagnosis of severe mental illness and substance use disorders, 1996-2010, Israel.

PubMed

Ponizovsky, Alexander M; Rosca, Paola; Haklai, Ziona; Goldberger, Nehama

2015-03-01

(1) To examine the trends in the incidence of dual diagnosis (DD) of severe mental illness and substance-related disorders among inpatients in Israel, and (2) the demographic and clinical correlates of DD patients. Using data from the National Psychiatric Case Register, we identified 56,774 inpatients aged 15-64 whose first psychiatric hospitalization occurred between 1996 and 2010. We compared the characteristics of inpatients having DD with drugs, alcohol or drug/alcohol abuse with those with mental disorder only. Over the period, DD with drugs decreased from 8.2% in 1996 to 6% in 2010; DD with alcohol increased from 3% to 4% and DD with drugs/alcohol from 2% to 4%. DD with drugs was highest, whereas DD with alcohol was lowest for the youngest age- group in 1996 but increased to the same as other age-groups in 2006-2010. Male gender, a previous suicide attempt, compulsory hospitalizations and marital status were positive predictors for all DD. Immigrant status was a positive predictor of DD with alcohol, but the opposite for DD with drugs; being Jewish and psychotic diagnosis was a positive predictor of DD with drugs, but negative for DD with alcohol. Over the study period, DD with drugs has decreased among young patients, although it is still higher than among older groups. However, DD with alcohol or drugs/alcohol has increased in the younger group. The clinical-demographic profile of DD patients was similar to that from the relevant literature, except for immigrant status that was negatively associated with DD with drugs. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Platelet-derived growth factor-DD targeting arrests pathological angiogenesis by modulating glycogen synthase kinase-3beta phosphorylation.

PubMed

Kumar, Anil; Hou, Xu; Lee, Chunsik; Li, Yang; Maminishkis, Arvydas; Tang, Zhongshu; Zhang, Fan; Langer, Harald F; Arjunan, Pachiappan; Dong, Lijin; Wu, Zhijian; Zhu, Linda Y; Wang, Lianchun; Min, Wang; Colosi, Peter; Chavakis, Triantafyllos; Li, Xuri

2010-05-14

Platelet-derived growth factor-DD (PDGF-DD) is a recently discovered member of the PDGF family. The role of PDGF-DD in pathological angiogenesis and the underlying cellular and molecular mechanisms remain largely unexplored. In this study, using different animal models, we showed that PDGF-DD expression was up-regulated during pathological angiogenesis, and inhibition of PDGF-DD suppressed both choroidal and retinal neovascularization. We also demonstrated a novel mechanism mediating the function of PDGF-DD. PDGF-DD induced glycogen synthase kinase-3beta (GSK3beta) Ser(9) phosphorylation and Tyr(216) dephosphorylation in vitro and in vivo, leading to increased cell survival. Consistently, GSK3beta activity was required for the antiangiogenic effect of PDGF-DD targeting. Moreover, PDGF-DD regulated the expression of GSK3beta and many other genes important for angiogenesis and apoptosis. Thus, we identified PDGF-DD as an important target gene for antiangiogenic therapy due to its pleiotropic effects on vascular and non-vascular cells. PDGF-DD inhibition may offer new therapeutic options to treat neovascular diseases.

Basic numerical processing, calculation, and working memory in children with dyscalculia and/or ADHD symptoms.

PubMed

Kuhn, Jörg-Tobias; Ise, Elena; Raddatz, Julia; Schwenk, Christin; Dobel, Christian

2016-09-01

Deficits in basic numerical skills, calculation, and working memory have been found in children with developmental dyscalculia (DD) as well as children with attention-deficit/hyperactivity disorder (ADHD). This paper investigates cognitive profiles of children with DD and/or ADHD symptoms (AS) in a double dissociation design to obtain a better understanding of the comorbidity of DD and ADHD. Children with DD-only (N = 33), AS-only (N = 16), comorbid DD+AS (N = 20), and typically developing controls (TD, N = 40) were assessed on measures of basic numerical processing, calculation, working memory, processing speed, and neurocognitive measures of attention. Children with DD (DD, DD+AS) showed deficits in all basic numerical skills, calculation, working memory, and sustained attention. Children with AS (AS, DD+AS) displayed more selective difficulties in dot enumeration, subtraction, verbal working memory, and processing speed. Also, they generally performed more poorly in neurocognitive measures of attention, especially alertness. Children with DD+AS mostly showed an additive combination of the deficits associated with DD-only and A_Sonly, except for subtraction tasks, in which they were less impaired than expected. DD and AS appear to be related to largely distinct patterns of cognitive deficits, which are present in combination in children with DD+AS.

Physicochemical, mechanical and thermal properties of chitosan films with and without sorbitol.

PubMed

Liu, Mei; Zhou, Yibin; Zhang, Yang; Yu, Chen; Cao, Shengnan

2014-09-01

The effect of sorbitol on the physicochemical, mechanical and thermal properties of chitosan films with different degrees of deacetylation (DD; i.e., DD85% and DD95%) was investigated. The thickness, moisture content (MC), water solubility (WS) and water-vapor permeability (WVP) of the films were evaluated. Sorbitol addition reduced MC, increased WS and significantly (p<0.01) reduced WVP of both film types. DD95% films had lower MC and WVP, and higher WS than DD85% films. Static (thermomechanical analysis) and dynamic (dynamic mechanical analysis) tests indicated that sorbitol increased the strain and decreased stress for both DD films, but DD95% could sustain higher strain and DD85% could sustain higher stress. Thermogravimetrics analysis and differential scanning calorimetry showed that sorbitol elicited a lower degradation temperature for both films, and that DD95% films exhibited higher thermal stability than DD85% films. Copyright © 2014 Elsevier B.V. All rights reserved.

Resonant Interaction, Approximate Symmetry, and Electromagnetic Interaction (EMI) in Low Energy Nuclear Reactions (LENR)

NASA Astrophysics Data System (ADS)

Chubb, Scott

2007-03-01

Only recently (talk by P.A. Mosier-Boss et al, in this session) has it become possible to trigger high energy particle emission and Excess Heat, on demand, in LENR involving PdD. Also, most nuclear physicists are bothered by the fact that the dominant reaction appears to be related to the least common deuteron(d) fusion reaction,d+d ->α+γ. A clear consensus about the underlying effect has also been illusive. One reason for this involves confusion about the approximate (SU2) symmetry: The fact that all d-d fusion reactions conserve isospin has been widely assumed to mean the dynamics is driven by the strong force interaction (SFI), NOT EMI. Thus, most nuclear physicists assume: 1. EMI is static; 2. Dominant reactions have smallest changes in incident kinetic energy (T); and (because of 2), d+d ->α+γ is suppressed. But this assumes a stronger form of SU2 symmetry than is present; d+d ->α+γ reactions are suppressed not because of large changes in T but because the interaction potential involves EMI, is dynamic (not static), the SFI is static, and because the two incident deuterons must have approximate Bose Exchange symmetry and vanishing spin. A generalization of this idea involves a resonant form of reaction, similar to the de-excitation of an atom. These and related (broken gauge) symmetry EMI effects on LENR are discussed.

The Defense Resource Allocation Process. Revision. Selected Reading in National Security Decision Making

DTIC Science & Technology

1988-06-01

ion ternic with a o I ht Ily d if t r rrI t me ua rIjnn. "New obliygational 6tithority" (NOik) ccin mean th addit ional amount cong res appropi ictes e...oJnce t lie pro sid (bt yn j usIhe. IWt hor i z a t i An dd Appi Upr iat ion Sct S t he-(Y became(- Ic ’Iand Hit, I’iiac I mont P ha so -, or I In Cong ...party interests, tthe sational initere-st ha: .i t ...... qually t rou - 1Li1i nl in ih divers on ofdeteitse_ i source :; away from - gelal ne scurit y

Cold Regions - Environmental Testing of Individual Soldier Equipment

DTIC Science & Technology

2011-10-17

REPORT DOCUMENTATION PAGE Form Approved OMB No. 0704-0188 The public reporting burden for this collection of information is estimated to average 1 ...currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. 1 . REPORT DATE (DD-MM-YYYY) 17-10-2011 2. REPORT...TOP. 15. SUBJECT TERMS Soldier equipment HFE MANPRINT cold region environment CRTC 16. SECURITY

ddPCRclust - An R package and Shiny app for automated analysis of multiplexed ddPCR data.

PubMed

Brink, Benedikt G; Meskas, Justin; Brinkman, Ryan R

2018-03-09

Droplet digital PCR (ddPCR) is an emerging technology for quantifying DNA. By partitioning the target DNA into ∼20000 droplets, each serving as its own PCR reaction compartment, a very high sensitivity of DNA quantification can be achieved. However, manual analysis of the data is time consuming and algorithms for automated analysis of non-orthogonal, multiplexed ddPCR data are unavailable, presenting a major bottleneck for the advancement of ddPCR transitioning from low-throughput to high- throughput. ddPCRclust is an R package for automated analysis of data from Bio-Rad's droplet digital PCR systems (QX100 and QX200). It can automatically analyse and visualise multiplexed ddPCR experiments with up to four targets per reaction. Results are on par with manual analysis, but only take minutes to compute instead of hours. The accompanying Shiny app ddPCRvis provides easy access to the functionalities of ddPCRclust through a web-browser based GUI. R package: https://github.com/bgbrink/ddPCRclust; Interface: https://github.com/bgbrink/ddPCRvis/; Web: https://bibiserv.cebitec.uni-bielefeld.de/ddPCRvis/. bbrink@cebitec.uni-bielefeld.de.

77 FR 23322 - Proposed Information Collection (Report of General Information) Activity: Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-18

... Information. d. VA Form 21-0820c, Report of Defense Finance and Accounting Service (DFAS). e. VA Form 21-0820d.... VA Form 21-0820c, Report of Defense Finance and Accounting Service (DFAS)--2,500. e. VA Form 21-0820d... Nursing Home Information--30,000. d. VA Form 21-0820c, Report of Defense Finance and Accounting Service...

32 CFR 516.25 - DA Form 4.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 3 2010-07-01 2010-07-01 true DA Form 4. 516.25 Section 516.25 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY AID OF CIVIL AUTHORITIES AND PUBLIC RELATIONS LITIGATION Reporting Legal Proceedings to HQDA § 516.25 DA Form 4. (a) General. The DA Form 4 (See figure C-2...

48 CFR 236.604 - Performance evaluation.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Performance evaluation... Architect-Engineer Services 236.604 Performance evaluation. (a) Preparation of performance reports. Use DD Form 2631, Performance Evaluation (Architect-Engineer), instead of SF 1421. (2) Prepare a separate...

Roles of the C-terminal domains of human dihydrodiol dehydrogenase isoforms in the binding of substrates and modulators: probing with chimaeric enzymes.

PubMed Central

Matsuura, K; Hara, A; Deyashiki, Y; Iwasa, H; Kume, T; Ishikura, S; Shiraishi, H; Katagiri, Y

1998-01-01

Human liver dihydrodiol dehydrogenase (DD; EC 1.3.1.20) exists in isoforms (DD1, DD2 and DD4) composed of 323 amino acids. DD1 and DD2 share 98% amino acid sequence identity, but show lower identities (approx. 83%) with DD4, in which a marked difference is seen in the C-terminal ten amino acids. DD4 exhibits unique catalytic properties, such as the ability to oxidize both (R)- and (S)-alicyclic alcohols equally, high dehydrogenase activity for bile acids, potent inhibition by steroidal anti-inflammatory drugs and activation by sulphobromophthalein and clofibric acid derivatives. In this study, we have prepared chimaeric enzymes, in which we exchanged the C-terminal 39 residues between the two enzymes. Compared with DD1, CDD1-4 (DD1 with the C-terminal sequence of DD4) had increased kcat/Km values for 3alpha-hydroxy-5beta-androstanes and bile acids of 3-9-fold and decreased values for the other substrates by 5-100-fold. It also became highly sensitive to DD4 inhibitors such as phenolphthalein and hexoestrol. Another chimaeric enzyme, CDD4-1 (DD4 with the C-terminal sequence of DD1), showed the same (S)-stereospecificity for the alicyclic alcohols as DD1, had decreased kcat/Km values for bile acids with 7beta- or 12alpha-hydroxy groups by more than 120-fold and was resistant to inhibition by betamethasone. In addition, the activation effects of sulphobromophthalein and bezafibrate decreased or disappeared for CDD4-1. The recombinant DD4 with the His314-->Pro (the corresponding residue of DD1) mutation showed intermediate changes in the properties between those of wild-type DD4 and CDD4-1. The results indicate that the binding of substrates, inhibitors and activators to the enzymes is controlled by residues in their C-terminal domains; multiple residues co-ordinately act as determinants for substrate specificity and inhibitor sensitivity. PMID:9820821

32 CFR 1290.9 - Forms and reports.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 6 2010-07-01 2010-07-01 false Forms and reports. 1290.9 Section 1290.9 National Defense Other Regulations Relating to National Defense DEFENSE LOGISTICS AGENCY MISCELLANEOUS PREPARING AND PROCESSING MINOR OFFENSES AND VIOLATION NOTICES REFERRED TO U.S. DISTRICT COURTS § 1290.9...

32 CFR 1290.9 - Forms and reports.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 6 2011-07-01 2011-07-01 false Forms and reports. 1290.9 Section 1290.9 National Defense Other Regulations Relating to National Defense DEFENSE LOGISTICS AGENCY MISCELLANEOUS PREPARING AND PROCESSING MINOR OFFENSES AND VIOLATION NOTICES REFERRED TO U.S. DISTRICT COURTS § 1290.9...

Interpretation of Overall Colonic Transit in Defecation Disorders in Males and Females

PubMed Central

Shin, Andrea; Camilleri, Michael; Nadeau, Ashley; Nullens, Sara; Rhee, Jong Chul; Jeong, In Du; Burton, Duane D.

2013-01-01

Background There is little information regarding gender-specific measurements of colonic transit and anorectal function in patients with defecation disorders (DD). Aim To compare overall colonic transit by gender in DD. Methods In 407 patients with constipation due to DD diagnosed by a single gastroenterologist (1994– 2012), DD was characterized by anorectal manometry, balloon expulsion test, and colonic transit by scintigraphy. The primary endpoint was overall colonic transit (geometric center, GC) at 24hours (GC24). Effects of gender in DD on colonic transit, and comparison with transit in 208 healthy controls were assessed by Mann-Whitney rank sum test. Secondary endpoints were maximum anal resting (ARP) and squeeze (ASP) pressures. We also tested association of the physiological endpoints among DD females by pregnancy history and among DD patients by colectomy history. Results The DD patients were 67 males (M) and 340 females (F). Significant differences by gender in DD patients were observed in GC24 (median: M: 2.2; F: 1.8; p=0.01), ARP (median: M: 87.8mmHg; F: 82.4mmHg; p=0.04), and ASP (median: M: 182.4mmHg; F: 128.7mmHg; p<0.001). GC24 was slower in DD compared to same gender healthy controls. GC24 did not differ among DD females by pregnancy history. Anorectal functions and upper GI transit did not differ among DD patients by colectomy history. Conclusions Patients with DD have slower colonic transit compared to gender-matched controls. Among DD patients, males have higher ARP and ASP, and females have slower colonic transit. Although the clinical significance of these differences may be unclear, findings suggest that interpretation of these tests in suspected DD should be based on same gender control data. PMID:23406422

Direct measurements of the non-DD cross section {sigma}{sub {psi}}{sub (3770){yields}}{sub non-DD} at E{sub cm}=3.773 GeV and the branching fraction for {psi}(3770){yields}non-DD

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ablikim, M.; Bai, J. Z.; Cai, X.

2007-12-15

By analyzing the data collected at the center-of-mass energy E{sub cm}=3.773 GeV and below the DD meson pair production threshold with the BES-II detector at the BEPC Collider, we directly measured the observed non-DD cross section of {psi}(3770) decay to be {sigma}{sub {psi}}{sub (3770){yields}}{sub non-DD}{sup obs}=(0.95{+-}0.35{+-}0.29) nb at E{sub cm}=3.773 GeV, and the branching fraction BF[{psi}(3770){yields}non-DD]=(13.4{+-}5.0{+-}3.6)% for inclusive non-DD decay of {psi}(3770). We also determined the cross section for DD meson pair production to be {sigma}{sub DD}{sup obs}=(6.12{+-}0.37{+-}0.23) nb at E{sub cm}=3.773 GeV.

Real time estimation of ship motions using Kalman filtering techniques

NASA Technical Reports Server (NTRS)

Triantafyllou, M. S.; Bodson, M.; Athans, M.

1983-01-01

The estimation of the heave, pitch, roll, sway, and yaw motions of a DD-963 destroyer is studied, using Kalman filtering techniques, for application in VTOL aircraft landing. The governing equations are obtained from hydrodynamic considerations in the form of linear differential equations with frequency dependent coefficients. In addition, nonminimum phase characteristics are obtained due to the spatial integration of the water wave forces. The resulting transfer matrix function is irrational and nonminimum phase. The conditions for a finite-dimensional approximation are considered and the impact of the various parameters is assessed. A detailed numerical application for a DD-963 destroyer is presented and simulations of the estimations obtained from Kalman filters are discussed.

Geoacoustic models of the Donghae-to-Gangneung region in the Korean continental margin of the East Sea

NASA Astrophysics Data System (ADS)

Ryang, Woo Hun; Kim, Seong Pil; Hahn, Jooyoung

2016-04-01

Geoacoustic model is to provide a model of the real seafloor with measured, extrapolated, and predicted values of geoacoustic environmental parameters. It controls acoustic propagation in underwater acoustics. In the Korean continental margin of the East Sea, this study reconstructed geoacoustic models using geoacoustic and marine geologic data of the Donghae-to-Gangneung region (37.4° to 37.8° in latitude). The models were based on the data of the high-resolution subbottom and air-gun seismic profiles with sediment cores. The Donghae region comprised measured P-wave velocities and attenuations of the cores, whereas the Gangneung region comprised regression values using measured values of the adjacent areas. Geoacoustic data of the cores were extrapolated down to a depth of the geoacoustic models. For actual modeling, the P-wave speed of the models was compensated to in situ depth below the sea floor using the Hamilton method. These geoacoustic models of this region probably contribute for geoacoustic and underwater acoustic modelling reflecting vertical and lateral variability of acoustic properties in the Korean continental margin of the western East Sea. Keywords: geoacoustic model, environmental parameter, East Sea, continental margin Acknowledgements: This research was supported by the research grants from the Agency of Defense Development (UD140003DD and UE140033DD).

Evaluation of diminished microbial contamination in handling of a novel daily disposable flat pack contact lens.

PubMed

Nomachi, Miya; Sakanishi, Kotaro; Ichijima, Hideji; Cavanagh, H Dwight

2013-05-01

To evaluate the efficacy of a novel daily disposable (DD) flat package in regard to microbial contamination on the anterior and posterior surfaces of a contact lens (CL) during handling of the lens for insertion. Four kinds of commercially available general blister-packed daily disposable contact lenses (DD CLs) as controls and a novel Magic 1-day Menicon Flat Pack as a test lens were used for this in vitro study. Lenses were removed from their packages using fingers coated with fluorescein 3 to 5 μm beads or an approximately 7×10(2) to 2×10(3) colony-forming unit (CFU)/mL Staphylococcus aureus suspension. The transfer of fluorescein beads to the surface of the lenses was then observed by fluorescence microscopy. Microbial contamination on the lenses was observed by light microscopy after a 2-day incubation period; and, the number of colonies isolated from the contaminated lenses was determined after 4 days of incubation. The number of fluorescein beads on the Magic lens was significantly less (p<0.05) than that of the general blister-packed control lenses. Adherence of microbial colonies was observed on both inner and outer surfaces of general blister-packed lenses, whereas no colony formation was found on the inner surface of the Magic lens, and the lowest bacterial adherence was observed for the Magic lens. The data demonstrated that placement of the Magic DD lens onto the eye is accompanied by diminished microbial contamination compared with general blister-packed DD CLs. Eye care professionals; however, should instruct patients to comply with intended use of DD CLs to prevent CL-associated microbial keratitis. In all cases, hand washing is mandated prelens insertion.

A comparison of pregnancy outcomes in Ghanaian women with varying dietary diversity: a prospective cohort study protocol

PubMed Central

Saaka, Mahama; Siassi, Fereydoun; Qorbani, Mostafa; Yavari, Parvaneh; Danquah, Ina; Sotoudeh, Gity

2016-01-01

Introduction Poor dietary intake during pregnancy can have negative repercussions on the mother and fetus. This study therefore aims to explore the dietary diversity (DD) of pregnant women and its associations with pregnancy outcomes among women in Northern Ghana. The main outcome variables to be measured are gestational weight gain and birth weight. Methods and analysis A prospective cohort study design will be used and 600 pregnant women in their first trimester will be systematically recruited at health facilities and followed until delivery. In three follow-up visits after recruitment, information on sociodemographic and general characteristics, physical activity (International Physical Activity Questionnaire (IPAQ) short form, dietary intake (24-hour food recall), anthropometry and pregnancy outcomes will be collected. DD will be measured three times using the minimum DD-women (MDD-W) indicator and the mean of the three values overall will be used to determine low (<5 food groups) and high (≥5 food groups) DD. Data will be analysed using SPSS. Comparisons between groups (categorical data) will be made using the χ2 test for proportions, and t-tests and ANOVA will be performed on continuous variables. Regression analysis will be used to identify independent outcome predictors while controlling for possible confounding factors. The results may help to identify differences in DD between healthy and unhealthy pregnancy outcomes. Ethics and dissemination The study protocol has been approved by the ethics committee of Tehran University of Medical Sciences and the ethical review committee of the Tamale Teaching Hospital. Written informed consent will be obtained from all subjects. The results will be published in due course. PMID:27655259

Reduction of Carbon Dioxide by a Molybdenum-Containing Formate Dehydrogenase: A Kinetic and Mechanistic Study.

PubMed

Maia, Luisa B; Fonseca, Luis; Moura, Isabel; Moura, José J G

2016-07-20

Carbon dioxide accumulation is a major concern for the ecosystems, but its abundance and low cost make it an interesting source for the production of chemical feedstocks and fuels. However, the thermodynamic and kinetic stability of the carbon dioxide molecule makes its activation a challenging task. Studying the chemistry used by nature to functionalize carbon dioxide should be helpful for the development of new efficient (bio)catalysts for atmospheric carbon dioxide utilization. In this work, the ability of Desulfovibrio desulfuricans formate dehydrogenase (Dd FDH) to reduce carbon dioxide was kinetically and mechanistically characterized. The Dd FDH is suggested to be purified in an inactive form that has to be activated through a reduction-dependent mechanism. A kinetic model of a hysteretic enzyme is proposed to interpret and predict the progress curves of the Dd FDH-catalyzed reactions (initial lag phase and subsequent faster phase). Once activated, Dd FDH is able to efficiently catalyze, not only the formate oxidation (kcat of 543 s(-1), Km of 57.1 μM), but also the carbon dioxide reduction (kcat of 46.6 s(-1), Km of 15.7 μM), in an overall reaction that is thermodynamically and kinetically reversible. Noteworthy, both Dd FDH-catalyzed formate oxidation and carbon dioxide reduction are completely inactivated by cyanide. Current FDH reaction mechanistic proposals are discussed and a different mechanism is here suggested: formate oxidation and carbon dioxide reduction are proposed to proceed through hydride transfer and the sulfo group of the oxidized and reduced molybdenum center, Mo(6+)═S and Mo(4+)-SH, are suggested to be the direct hydride acceptor and donor, respectively.

Insertion/deletion polymorphism of ACE gene in females with peripartum cardiomyopathy: A case-control study.

PubMed

Yaqoob, Irfan; Tramboo, Nisar A; Bhat, Irfan A; Pandith, Arshad; Beig, Jahangir R; Hafeez, Imran; Lone, Aijaz A; Shah, Tariq R; Samreen, Sumera

The role of polymorphism of Angiotensin converting enzyme (ACE) gene and ACE activity in etiopathogenesis, prognosis, and many other clinical parameters in the various form of the cardiovascular disease has been established to some degree of certainty. The pathophysiology of Peripartum cardiomyopathy (PPCM) remains an area of active research. The main aim of our study was to see pattern of ACE- Insertion/Deletion (I/D) allele in PPCM and its implications on left ventricular performance indices. This single-center case-control study included 45 cases and 70 controls. The diagnosis of PPCM was established clinically and echocardiographically. ACE genotyping was done by polymerase chain reaction (PCR) method in all subjects. The II, ID, and DD genotype was present in 16, 18 and 11 of subjects with PPCM and 48, 19 and 3 of controls respectively. The odds ratio for ACE-II genotype in cases vs. controls was 0.253 (95% CI=0.114-0.558; p=0.007), for that of II genotype was 1.93 (95% CI=0.86-4.3; p=0.107) and for DD genotype was 7.225 (95% CI; 1.88-27.6; p=0.0039). Overall frequency of D allele in cases was significantly higher than controls (odds=4.25; 95% CI=2.01-6.7; p=0.0001). Moreover, ejection fraction, left ventricular volume and linear dimensions were worse in patients with DD genotype. ACE DD genotype and overall frequency of D allele is significantly higher in patients with PPCM. Also, the presence of DD genotype is associated with worse systolic performance indices measured echocardiographically. Copyright © 2017. Published by Elsevier B.V.

Integrated roles of BclA and DD-carboxypeptidase 1 in Bradyrhizobium differentiation within NCR-producing and NCR-lacking root nodules.

PubMed

Barrière, Quentin; Guefrachi, Ibtissem; Gully, Djamel; Lamouche, Florian; Pierre, Olivier; Fardoux, Joël; Chaintreuil, Clémence; Alunni, Benoît; Timchenko, Tatiana; Giraud, Eric; Mergaert, Peter

2017-08-22

Legumes harbor in their symbiotic nodule organs nitrogen fixing rhizobium bacteria called bacteroids. Some legumes produce Nodule-specific Cysteine-Rich (NCR) peptides in the nodule cells to control the intracellular bacterial population. NCR peptides have antimicrobial activity and drive bacteroids toward terminal differentiation. Other legumes do not produce NCR peptides and their bacteroids are not differentiated. Bradyrhizobia, infecting NCR-producing Aeschynomene plants, require the peptide uptake transporter BclA to cope with the NCR peptides as well as a specific peptidoglycan-modifying DD-carboxypeptidase, DD-CPase1. We show that Bradyrhizobium diazoefficiens strain USDA110 forms undifferentiated bacteroids in NCR-lacking soybean nodules. Unexpectedly, in Aeschynomene afraspera nodules the nitrogen fixing USDA110 bacteroids are hardly differentiated despite the fact that this host produces NCR peptides, suggesting that USDA110 is insensitive to the host peptide effectors and that nitrogen fixation can be uncoupled from differentiation. In agreement with the absence of bacteroid differentiation, USDA110 does not require its bclA gene for nitrogen fixing symbiosis with these two host plants. Furthermore, we show that the BclA and DD-CPase1 act independently in the NCR-induced morphological differentiation of bacteroids. Our results suggest that BclA is required to protect the rhizobia against the NCR stress but not to induce the terminal differentiation pathway.

A dual small-molecule rheostat for precise control of protein concentration in Mammalian cells.

PubMed

Lin, Yu Hsuan; Pratt, Matthew R

2014-04-14

One of the most successful strategies for controlling protein concentrations in living cells relies on protein destabilization domains (DD). Under normal conditions, a DD will be rapidly degraded by the proteasome. However, the same DD can be stabilized or "shielded" in a stoichiometric complex with a small molecule, enabling dose-dependent control of its concentration. This process has been exploited by several labs to post-translationally control the expression levels of proteins in vitro as well as in vivo, although the previous technologies resulted in permanent fusion of the protein of interest to the DD, which can affect biological activity and complicate results. We previously reported a complementary strategy, termed traceless shielding (TShld), in which the protein of interest is released in its native form. Here, we describe an optimized protein concentration control system, TTShld, which retains the traceless features of TShld but utilizes two tiers of small molecule control to set protein concentrations in living cells. These experiments provide the first protein concentration control system that results in both a wide range of protein concentrations and proteins free from engineered fusion constructs. The TTShld system has a greatly improved dynamic range compared to our previously reported system, and the traceless feature is attractive for elucidation of the consequences of protein concentration in cell biology. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Interplay of Dzyaloshinsky-Moriya and dipole-dipole interactions and their joint effects upon vortical structures on nanodisks

NASA Astrophysics Data System (ADS)

Liu, Zhaosen; Ciftja, Orion; Ian, Hou

2017-06-01

In transition metal oxides, magnetic dipole-dipole (DD) and chiral Dzyaloshinsky-Moriya (DM) interactions between nearest neighboring spins are comparable in magnitude. In particular, the effects of the DD interaction on the physical properties of magnetic nanosystems cannot be simply neglected due to its long-range character. For these reasons, we employed here a new quantum simulation approach in order to investigate the interplay of these two interactions and study their combined effects upon the magnetic vortical structures of monolayer nanodisks. Consequently, we found out from our computational results that, in the presence of Heisenberg exchange interaction, a sufficiently strong DD interaction is also able to induce a single magnetic vortex on a small nanodisk; a strong DM interaction usually gives rise to a multi-domain structure which evolves with changing temperature; In this circumstance, if a weak DD interaction is further considered, the multi-domains merge to form a single vortex in the whole magnetic phase. Moreover, if only the Heisenberg exchange and chiral DM interactions are considered in simulations, our results from calculations with different spin values show that the transition temperature TM is simply proportional to S (S + 1) ; if the temperature is scaled with TM, and the calculated magnetizations are divided by the spin value S, their curves exhibit very similar features in the whole temperature region below TM.

32 CFR 903.10 - Information collections, records, and forms or information management tools (IMTS).

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 6 2010-07-01 2010-07-01 false Information collections, records, and forms or information management tools (IMTS). 903.10 Section 903.10 National Defense Department of Defense (Continued... Information collections, records, and forms or information management tools (IMTS). (a) Information...

32 CFR 903.10 - Information collections, records, and forms or information management tools (IMTS).

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 6 2014-07-01 2014-07-01 false Information collections, records, and forms or information management tools (IMTS). 903.10 Section 903.10 National Defense Department of Defense (Continued... Information collections, records, and forms or information management tools (IMTS). (a) Information...

32 CFR 903.10 - Information collections, records, and forms or information management tools (IMTS).

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 6 2013-07-01 2013-07-01 false Information collections, records, and forms or information management tools (IMTS). 903.10 Section 903.10 National Defense Department of Defense (Continued... Information collections, records, and forms or information management tools (IMTS). (a) Information...

32 CFR 903.10 - Information collections, records, and forms or information management tools (IMTS).

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 6 2011-07-01 2011-07-01 false Information collections, records, and forms or information management tools (IMTS). 903.10 Section 903.10 National Defense Department of Defense (Continued... Information collections, records, and forms or information management tools (IMTS). (a) Information...

32 CFR 903.10 - Information collections, records, and forms or information management tools (IMTS).

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 6 2012-07-01 2012-07-01 false Information collections, records, and forms or information management tools (IMTS). 903.10 Section 903.10 National Defense Department of Defense (Continued... Information collections, records, and forms or information management tools (IMTS). (a) Information...

Compressed 3D and 2D digital images versus standard 3D slide film for the evaluation of glaucomatous optic nerve features.

PubMed

Sandhu, Simrenjeet; Rudnisky, Chris; Arora, Sourabh; Kassam, Faazil; Douglas, Gordon; Edwards, Marianne C; Verstraten, Karin; Wong, Beatrice; Damji, Karim F

2018-03-01

Clinicians can feel confident compressed three-dimensional digital (3DD) and two-dimensional digital (2DD) imaging evaluating important features of glaucomatous disc damage is comparable to the previous gold standard of stereoscopic slide film photography, supporting the use of digital imaging for teleglaucoma applications. To compare the sensitivity and specificity of 3DD and 2DD photography with stereo slide film in detecting glaucomatous optic nerve head features. This prospective, multireader validation study imaged and compressed glaucomatous, suspicious or normal optic nerves using a ratio of 16:1 into 3DD and 2DD (1024×1280 pixels) and compared both to stereo slide film. The primary outcome was vertical cup-to-disc ratio (VCDR) and secondary outcomes, including disc haemorrhage and notching, were also evaluated. Each format was graded randomly by four glaucoma specialists. A protocol was implemented for harmonising data including consensus-based interpretation as needed. There were 192 eyes imaged with each format. The mean VCDR for slide, 3DD and 2DD was 0.59±0.20, 0.60±0.18 and 0.62±0.17, respectively. The agreement of VCDR for 3DD versus film was κ=0.781 and for 2DD versus film was κ=0.69. Sensitivity (95.2%), specificity (95.2%) and area under the curve (AUC; 0.953) of 3DD imaging to detect notching were better (p=0.03) than for 2DD (90.5%; 88.6%; AUC=0.895). Similarly, sensitivity (77.8%), specificity (98.9%) and AUC (0.883) of 3DD to detect disc haemorrhage were better (p=0.049) than for 2DD (44.4%; 99.5%; AUC=0.72). There was no difference between 3DD and 2DD imaging in detecting disc tilt (p=0.7), peripapillary atrophy (p=0.16), grey crescent (p=0.1) or pallor (p=0.43), although 3D detected sloping better (p=0.013). Both 3DD and 2DD imaging demonstrates excellent reproducibility in comparison to stereo slide film with experts evaluating VCDR, notching and disc haemorrhage. 3DD in this study was slightly more accurate than 2DD for evaluating disc haemorrhage, notching and sloping. © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2018. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

40 CFR Table 2 to Subpart Dd of... - Applicability of Paragraphs in Subpart A of This Part 63-General Provisions to Subpart DD

Code of Federal Regulations, 2010 CFR

2010-07-01

... A of This Part 63-General Provisions to Subpart DD 2 Table 2 to Subpart DD of Part 63 Protection of... Hazardous Air Pollutants from Off-Site Waste and Recovery Operations Pt. 63, Subpt. DD, Table 2 Table 2 to Subpart DD of Part 63—Applicability of Paragraphs in Subpart A of This Part 63—General Provisions to...

Definitive Results of a Phase III Adjuvant Trial Comparing Three Chemotherapy Regimens in Women With Operable, Node-Positive Breast Cancer: The NSABP B-38 Trial

PubMed Central

Swain, Sandra M.; Tang, Gong; Geyer, Charles E.; Rastogi, Priya; Atkins, James N.; Donnellan, Paul P.; Fehrenbacher, Louis; Azar, Catherine A.; Robidoux, André; Polikoff, Jonathan A.; Brufsky, Adam M.; Biggs, David D.; Levine, Edward A.; Zapas, John L.; Provencher, Louise; Northfelt, Donald W.; Paik, Soonmyung; Costantino, Joseph P.; Mamounas, Eleftherios P.; Wolmark, Norman

2013-01-01

Purpose Anthracycline- and taxane-based three-drug chemotherapy regimens have proven benefit as adjuvant therapy for early-stage breast cancer. This trial (NSABP B-38; Combination Chemotherapy in Treating Women Who Have Undergone Surgery for Node-Positive Breast Cancer) asked whether the incorporation of a fourth drug could improve outcomes relative to two standard regimens and provided a direct comparison of those two regimens. Patients and Methods We randomly assigned 4,894 women with node-positive early-stage breast cancer to six cycles of docetaxel, doxorubicin, and cyclophosphamide (TAC), four cycles of dose-dense (DD) doxorubicin and cyclophosphamide followed by four cycles of DD paclitaxel (P; DD AC→P), or DD AC→P with four cycles of gemcitabine (G) added to the DD paclitaxel (DD AC→PG). Primary granulocyte colony-stimulating factor support was required; erythropoiesis-stimulating agents (ESAs) were used at the investigator's discretion. Results There were no significant differences in 5-year disease-free survival (DFS) between DD AC→PG and DD AC→P (80.6% v 82.2%; HR, 1.07; P = .41), between DD AC→PG and TAC (80.6% v 80.1%; HR, 0.93; P = .39), in 5-year overall survival (OS) between DD AC→PG and DD AC→P (90.8% v 89.1%; HR, 0.85; P = .13), between DD AC→PG and TAC (90.8% v 89.6%; HR, 0.86; P = .17), or between DD AC→P versus TAC for DFS (HR, 0.87; P = .07) and OS (HR, 1.01; P = .96). Grade 3 to 4 toxicities for TAC, DD AC→P, and DD AC→PG, respectively, were febrile neutropenia (9%, 3%, 3%; P < .001), sensory neuropathy (< 1%, 7%, 6%; P < .001), and diarrhea (7%, 2%, 2%; P < .001). Exploratory analyses for ESAs showed no association with DFS events (HR, 1.02; P = .95). Conclusion Adding G to DD AC→P did not improve outcomes. No significant differences in efficacy were identified between DD AC→P and TAC, although toxicity profiles differed. PMID:23940225

Definitive results of a phase III adjuvant trial comparing three chemotherapy regimens in women with operable, node-positive breast cancer: the NSABP B-38 trial.

PubMed

Swain, Sandra M; Tang, Gong; Geyer, Charles E; Rastogi, Priya; Atkins, James N; Donnellan, Paul P; Fehrenbacher, Louis; Azar, Catherine A; Robidoux, André; Polikoff, Jonathan A; Brufsky, Adam M; Biggs, David D; Levine, Edward A; Zapas, John L; Provencher, Louise; Northfelt, Donald W; Paik, Soonmyung; Costantino, Joseph P; Mamounas, Eleftherios P; Wolmark, Norman

2013-09-10

Anthracycline- and taxane-based three-drug chemotherapy regimens have proven benefit as adjuvant therapy for early-stage breast cancer. This trial (NSABP B-38; Combination Chemotherapy in Treating Women Who Have Undergone Surgery for Node-Positive Breast Cancer) asked whether the incorporation of a fourth drug could improve outcomes relative to two standard regimens and provided a direct comparison of those two regimens. We randomly assigned 4,894 women with node-positive early-stage breast cancer to six cycles of docetaxel, doxorubicin, and cyclophosphamide (TAC), four cycles of dose-dense (DD) doxorubicin and cyclophosphamide followed by four cycles of DD paclitaxel (P; DD AC→P), or DD AC→P with four cycles of gemcitabine (G) added to the DD paclitaxel (DD AC→PG). Primary granulocyte colony-stimulating factor support was required; erythropoiesis-stimulating agents (ESAs) were used at the investigator's discretion. There were no significant differences in 5-year disease-free survival (DFS) between DD AC→PG and DD AC→P (80.6% v 82.2%; HR, 1.07; P = .41), between DD AC→PG and TAC (80.6% v 80.1%; HR, 0.93; P = .39), in 5-year overall survival (OS) between DD AC→PG and DD AC→P (90.8% v 89.1%; HR, 0.85; P = .13), between DD AC→PG and TAC (90.8% v 89.6%; HR, 0.86; P = .17), or between DD AC→P versus TAC for DFS (HR, 0.87; P = .07) and OS (HR, 1.01; P = .96). Grade 3 to 4 toxicities for TAC, DD AC→P, and DD AC→PG, respectively, were febrile neutropenia (9%, 3%, 3%; P < .001), sensory neuropathy (< 1%, 7%, 6%; P < .001), and diarrhea (7%, 2%, 2%; P < .001). Exploratory analyses for ESAs showed no association with DFS events (HR, 1.02; P = .95). Adding G to DD AC→P did not improve outcomes. No significant differences in efficacy were identified between DD AC→P and TAC, although toxicity profiles differed.

32 CFR 199.20 - Continued Health Care Benefit Program (CHCBP).

Code of Federal Regulations, 2011 CFR

2011-07-01

... attained the age of 21 if not in school or age 23 if enrolled in a full time course of study at an... Privilege Card”; (iii) A front and back copy of a DD Form 1173, “Uniformed Services Identification and...

48 CFR 236.201 - Evaluation of contractor performance.

Code of Federal Regulations, 2010 CFR

2010-10-01

... CONTRACTS Special Aspects of Contracting for Construction 236.201 Evaluation of contractor performance. (a) Preparation of performance evaluation reports. Use DD Form 2626, Performance Evaluation (Construction... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Evaluation of contractor...

48 CFR 5108.070 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-10-01

... accompanying Industrial Preparedness Program Production Capacity Survey (DD Form 1519 TEST). The firm is... is contractually bound by inclusion of AFARS 5152.208-9001 in their contract to maintain production capacity for a negotiated length of time, to conduct subcontractor planning, and to produce specified...

48 CFR 5108.070 - Definitions.

Code of Federal Regulations, 2012 CFR

2012-10-01

... accompanying Industrial Preparedness Program Production Capacity Survey (DD Form 1519 TEST). The firm is... is contractually bound by inclusion of AFARS 5152.208-9001 in their contract to maintain production capacity for a negotiated length of time, to conduct subcontractor planning, and to produce specified...

48 CFR 5108.070 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-10-01

... accompanying Industrial Preparedness Program Production Capacity Survey (DD Form 1519 TEST). The firm is... is contractually bound by inclusion of AFARS 5152.208-9001 in their contract to maintain production capacity for a negotiated length of time, to conduct subcontractor planning, and to produce specified...

48 CFR 5108.070 - Definitions.

Code of Federal Regulations, 2011 CFR

2011-10-01

... accompanying Industrial Preparedness Program Production Capacity Survey (DD Form 1519 TEST). The firm is... is contractually bound by inclusion of AFARS 5152.208-9001 in their contract to maintain production capacity for a negotiated length of time, to conduct subcontractor planning, and to produce specified...

Magnetocaloric Effect and Thermoelectric Cooling - A Synergistic Cooling Technology

DTIC Science & Technology

2018-01-16

REPORT DOCUMENTATION PAGE Form Approved 0 MB No. 0704-01 88 The public reporting burden for this collection of information is estimated to average...currently valid 0MB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. 1. REPORT DATE (DD-MM-YYYY) 12. REPORT TYPE 3. DATES COVERED...code) 858-736-5069 Standard Form 298 (Rev. 8198) Prescribed by ANSI Std. Z39.18 GENERAL ENGINEERING AND RESEARCH Final Technical Report

ENVIRONMENTAL QUALITY, ENERGY, AND POWER TECHNOLOGY Task Order 0012: Plug In Electric Vehicle, Vehicle to Grid

DTIC Science & Technology

2017-12-05

Form Approved OMB No. 0704-0188 The public reporting burden for this collection of information is estimated to average 1 hour per response...number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. 1. REPORT DATE (DD-MM-YY) 2. REPORT TYPE 3. DATES COVERED (From - To) 05 December...THIS PAGE Unclassified Thomas Layne 19b. TELEPHONE NUMBER (Include Area Code) (937) 255-2693 Standard Form 298 (Rev. 8-98

Critical Thinking, Army Design Methodology, And The Climate Change Policy Debate

DTIC Science & Technology

2016-05-26

Staff College Fort Leavenworth, Kansas 2016 REPORT DOCUMENTATION PAGE Form Approved OMB No. 0704-0188 Public reporting burden for this collection of...information if it does not display a currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. 1. REPORT DATE (DD-MM...Standard Form 298 (Rev. 8-98) Prescribed by ANSI Std. Z39.18 Fair use determination or copyright permission has been obtained for the inclusion

Building 65 Hydraulic Systems Handbook: Components, Systems, and Applications

DTIC Science & Technology

2016-04-01

blocks. 1 REPORT DOCUMENTATION PAGE Form Approved OMB No. 0704-0188 The public reporting burden for this collection of information is estimated...display a currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. 1. REPORT DATE (DD-MM-YY) 2. REPORT TYPE 3. DATES...Include Area Code) N/A Standard Form 298 (Rev. 8-98) Prescribed by ANSI Std. Z39-18 i DISTRIBUTION STATEMENT A: Approved for public release

Computational Study of the Structure and Mechanical Properties of the Molecular Crystal RDX

DTIC Science & Technology

2012-11-01

ii REPORT DOCUMENTATION PAGE Form Approved OMB No. 0704-0188 Public reporting burden for this collection of information is estimated to...currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. 1. REPORT DATE (DD-MM-YYYY) November 2012 2. REPORT TYPE...Unclassified 19b. TELEPHONE NUMBER (Include area code) (410) 278-7511 Standard Form 298 (Rev. 8/98) Prescribed by ANSI Std. Z39.18 iii Contents

Global Ionospheric Processes

DTIC Science & Technology

2008-10-29

DOCUMENTATION PAGE Form ApprovedOMB No. 0704-01-0188 The public reporting burden for this collection of information is estimated to average 1 hour per response...person shall be PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. 1 . REPORT DATE (DD-MM-YYYY) 2. REPORT TYPE 3. DATES COVERED (From - To) 4. TITLE...iii Contents 1 . INTRODUCTION 1 1.1 Task History and Overview 1 1.2 Objectives

Test Operations Procedure (TOP) 2-2-650 Engine Cold-Starting and Warm-Up Tests

DTIC Science & Technology

2008-02-12

REPORT DOCUMENTATION PAGE Form Approved OMB No. 0704-0188 The public reporting burden for this collection of information is estimated to average 1 ...currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. 1 . REPORT DATE (DD-MM-YYYY) 12-02-2008 2. REPORT...Page Paragraph 1 . SCOPE................................................................................ 2 2. FACILITIES AND INSTRUMENTATION

Patients with IgA nephropathy exhibit high systemic PDGF-DD levels.

PubMed

Boor, Peter; Eitner, Frank; Cohen, Clemens D; Lindenmeyer, Maja T; Mertens, Peter R; Ostendorf, Tammo; Floege, Jürgen

2009-09-01

Platelet-derived growth factor (PDGF) is a central mediator of mesangioproliferative glomerulonephritis (GN). In experimental mesangioproliferative GN, PDGF-DD serum levels, unlike PDGF-BB, increased up to 1000-fold. We assessed disease activity in 72 patients with GN, established a novel PDGF-D ELISA and then determined their PDGF-DD levels. In parallel, we studied renal PDGF-DD mRNA expression by RT-PCR. PDGF-DD serum levels in patients with IgA nephropathy (IgAN) were significantly higher (1.67 +/- 0.45 ng/ml) and in patients with lupus nephritis significantly lower (0.66 +/- 0.86 ng/ml) compared to healthy controls (1.17 +/- 0.46 ng/ml), while patients with focal segmental glomerulosclerosis, membranous GN and ANCA-positive vasculitis did not differ from controls. The subgroup of IgAN patients with elevated PDGF-DD levels (27% of samples) did not differ in their clinical features from those with normal PDGF-DD levels. In IgAN patients with repetitive PDGF-DD determinations, most exhibited only minor fluctuations of serum levels over time. Intrarenal PDGF-DD mRNA expression did not differ between controls and patients, suggesting an extrarenal source of the elevated PDGF-DD in IgAN. Serum PDGF-DD levels were specifically elevated in patients with IgAN, in particular in those with early disease, i.e. preserved renal function. Our data support the rationale for anti-PDGF-DD therapy in mesangioproliferative GN.

Original Article: Preeclampsia, Placental Insufficiency and Autism Spectrum Disorder or Developmental Delay

PubMed Central

Walker, Cheryl K.; Krakowiak, Paula; Baker, Alice; Hansen, Robin L.; Ozonoff, Sally; Hertz-Picciotto, Irva

2014-01-01

Importance Increasing evidence suggests that autism spectrum disorder (ASD) and many forms of developmental delay (DD) originate during fetal development. Preeclampsia may trigger aberrant neurodevelopment through placental, maternal and fetal physiologic mechanisms. Objective To determine whether preeclampsia is associated with ASD and/or DD. Design, Setting and Participants The CHildhhood Autism Risks from Genetics and the Environment (CHARGE) Study is a population-based case-control investigation of ASD and/or DD origins. Children from 20 California counties aged 24-60 months at the time of recruitment, and living in catchment areas with a biologic parent fluent in English or Spanish were enrolled from January 29, 2003 through April 7, 2011. Children with ASD (n=517) and DD (n=194) were recruited through the California Department of Developmental Services, the Medical Investigation of Neurodevelopmental Disorders (MIND) Institute and referrals. Controls with typical development (TD) controls (n=350) were randomly selected from birth records and frequency-matched on age, sex, and broad geographic region. Physicians diagnosing preeclampsia were masked to neurodevelopmental outcome, and those assessing neurodevelopmental function were masked to preeclampsia status. Exposure Preeclampsia and placental insufficiency were self-reported and abstracted from medical records. Main Outcome Measure The Autism Diagnostic Observation Schedule and Autism Diagnostic Interview–Revised were used to confirm ASD, whereas children with DD and TD were confirmed by Mullen Scales of Early Learning and Vineland Adaptive Behavior Scales and were free of autistic symptoms. Hypotheses were formulated before data collection. Results Children with ASD were twice as likely to have been exposed in utero to preeclampsia as controls with TD after adjustment for maternal educational level, parity, and prepregnancy obesity (adjusted odds ratio, 2.36; 95% CI, 1.18-4.68); risk increased with greater preeclampsia severity (test for trend p=0.02). Placental insufficiency appeared responsible for the increase in DD risk associated with severe preeclampsia (adjusted odds ratio, 5.49; 95% CI, 2.06-14.64). Conclusions and Relevance Preeclampsia, particularly severe disease, is associated with ASD and DD. Faulty placentation manifests in the mother as preeclampsia with vascular damage, enhanced systemic inflammation and insulin resistance; in the placenta as oxygen and nutrient transfer restriction and oxidative stress; and in the fetus as growth restriction and progressive hypoxemia. All are potential mechanisms for neurodevelopmental compromise. PMID:25485869

Contractile Ring-independent Localization of DdINCENP, a Protein Important for Spindle Stability and CytokinesisD⃞V⃞

PubMed Central

Chen, Qian; Li, Hui; De Lozanne, Arturo

2006-01-01

Dictyostelium DdINCENP is a chromosomal passenger protein associated with centromeres, the spindle midzone, and poles during mitosis and the cleavage furrow during cytokinesis. Disruption of the single DdINCENP gene revealed important roles for this protein in mitosis and cytokinesis. DdINCENP null cells lack a robust spindle midzone and are hypersensitive to microtubule-depolymerizing drugs, suggesting that their spindles may not be stable. Furthermore DdCP224, a protein homologous to the microtubule-stabilizing protein TOGp/XMAP215, was absent from the spindle midzone of DdINCENP null cells. Overexpression of DdCP224 rescued the weak spindle midzone defect of DdINCENP null cells. Although not required for the localization of the myosin II contractile ring and subsequent formation of a cleavage furrow, DdINCENP is important for the abscission of daughter cells at the end of cytokinesis. Finally, we show that the localization of DdINCENP at the cleavage furrow is modulated by myosin II but it occurs by a mechanism different from that controlling the formation of the contractile ring. PMID:16339076

32 CFR 516.25 - DA Form 4.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 3 2013-07-01 2013-07-01 false DA Form 4. 516.25 Section 516.25 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY AID OF CIVIL AUTHORITIES AND PUBLIC... attached to a properly prepared and sealed DA Form 4 are self-authenticating. (See Fed. R. Evid. 902). (b...

32 CFR 516.25 - DA Form 4.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 3 2014-07-01 2014-07-01 false DA Form 4. 516.25 Section 516.25 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY AID OF CIVIL AUTHORITIES AND PUBLIC... attached to a properly prepared and sealed DA Form 4 are self-authenticating. (See Fed. R. Evid. 902). (b...

32 CFR 516.25 - DA Form 4.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 3 2011-07-01 2009-07-01 true DA Form 4. 516.25 Section 516.25 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY AID OF CIVIL AUTHORITIES AND PUBLIC RELATIONS... properly prepared and sealed DA Form 4 are self-authenticating. (See Fed. R. Evid. 902). (b) Preparation at...

32 CFR 516.25 - DA Form 4.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 3 2012-07-01 2009-07-01 true DA Form 4. 516.25 Section 516.25 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY AID OF CIVIL AUTHORITIES AND PUBLIC RELATIONS... properly prepared and sealed DA Form 4 are self-authenticating. (See Fed. R. Evid. 902). (b) Preparation at...

32 CFR 807.2 - Charges for publications and forms.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 6 2010-07-01 2010-07-01 false Charges for publications and forms. 807.2 Section 807.2 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE ADMINISTRATION SALE TO THE PUBLIC § 807.2 Charges for publications and forms. (a) The Air Force applies charges...

32 CFR 1901.12 - Requirements as to form.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 6 2013-07-01 2013-07-01 false Requirements as to form. 1901.12 Section 1901.12 National Defense Other Regulations Relating to National Defense CENTRAL INTELLIGENCE AGENCY PUBLIC RIGHTS UNDER THE PRIVACY ACT OF 1974 Filing of Privacy Act Requests § 1901.12 Requirements as to form. (a) In...

32 CFR 1901.12 - Requirements as to form.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 6 2011-07-01 2011-07-01 false Requirements as to form. 1901.12 Section 1901.12 National Defense Other Regulations Relating to National Defense CENTRAL INTELLIGENCE AGENCY PUBLIC RIGHTS UNDER THE PRIVACY ACT OF 1974 Filing of Privacy Act Requests § 1901.12 Requirements as to form. (a) In...

Angiotensin converting enzyme genotype and chronic allograft nephropathy in protocol biopsies.

PubMed

Hueso, Miguel; Alía, Pedro; Moreso, Francesc; Beltrán-Sastre, Violeta; Riera, Luis; González, Carlota; Navarro, Miguel Angel; Grinyó, Josep Maria; Navarro, Estanis; Serón, Daniel

2004-08-01

Genotype DD of the angiotensin-converting enzyme (ACE) is not associated with an increased incidence of native renal diseases, although it could modulate progression to renal failure in patients who already display chronic lesions. Because its role in renal allograft degeneration is not well characterized, whether ACE genotype was associated with the prevalence of chronic allograft nephropathy (CAN) was studied, in a group of protocol biopsies from 180 patients, or with the incidence of CAN in 152 patients with at least two sequential biopsies. As a control group, ACE genotype was also studied in 41 donors and 72 healthy subjects. For analyzing the influence of ACE genotype in graft survival, patients were grouped into six categories (II-normal biopsy, ID-normal, DD-normal, II-CAN, ID-CAN and DD-CAN). Finally, relative renal ACE mRNA levels were measured in 67 cases by real-time PCR using the delta threshold cycle method. ACE-DD genotype was more frequent in patients who received a transplant than in control subjects (43.3% versus 30.1%, P = 0.026), but prevalence (DD = 42.7% versus non-DD = 42.2%) or incidence (DD = 24.6% versus non-DD = 29.9%) of CAN was not different regarding recipient ACE genotype. Furthermore, patients with the ACE-DD genotype and CAN had the poorest graft survival (II-normal = 100%, ID-normal = 91%, DD-normal = 84%, II-CAN = 100%, ID-CAN = 66%, and DD-CAN = 36%; P = 0.034) and higher ACE mRNA levels than non-DD and CAN (DD = -3.36 +/- 2.35 versus non-DD = -5.65 +/- 1.72-fold in ACE copies; P = 0.012). It is concluded that ACE-DD genotype is not associated with an increased prevalence or incidence of CAN but is actually associated with higher ACE mRNA levels and poorer graft survival in patients who already display CAN.

12 CFR 230.3 - General disclosure requirements.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 230.3 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) BOARD OF GOVERNORS OF THE FEDERAL RESERVE SYSTEM TRUTH IN SAVINGS (REGULATION DD) § 230.3 General disclosure requirements. (a) Form. Depository... the consumer and the depository institution. Disclosures may be made in languages other than English...

48 CFR 1846.670-3 - Use.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Use. 1846.670-3 Section 1846.670-3 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE ADMINISTRATION CONTRACT MANAGEMENT QUALITY ASSURANCE Material Inspection and Receiving Reports 1846.670-3 Use. The DD Form...

32 CFR 634.46 - Point system procedures.

Code of Federal Regulations, 2011 CFR

2011-07-01

... CRIMINAL INVESTIGATIONS MOTOR VEHICLE TRAFFIC SUPERVISION Driving Records and the Traffic Point System § 634.46 Point system procedures. (a) Reports of moving traffic violations recorded on DD Form 1408 or... traffic points or the suspension or revocation of driving privileges on the person's driving record...

32 CFR 634.46 - Point system procedures.

Code of Federal Regulations, 2010 CFR

2010-07-01

... CRIMINAL INVESTIGATIONS MOTOR VEHICLE TRAFFIC SUPERVISION Driving Records and the Traffic Point System § 634.46 Point system procedures. (a) Reports of moving traffic violations recorded on DD Form 1408 or... traffic points or the suspension or revocation of driving privileges on the person's driving record...