Molecular contributions to conservation

USGS Publications Warehouse

Haig, Susan M.

1998-01-01

Recent advances in molecular technology have opened a new chapter in species conservation efforts, as well as population biology. DNA sequencing, MHC (major histocompatibility complex), minisatellite, microsatellite, and RAPD (random amplified polymorphic DNA) procedures allow for identification of parentage, more distant relatives, founders to new populations, unidentified individuals, population structure, effective population size, population-specific markers, etc. PCR (polymerase chain reaction) amplification of mitochondrial DNA, nuclear DNA, ribosomal DNA, chloroplast DNA, and other systems provide for more sophisticated analyses of metapopulation structure, hybridization events, and delineation of species, subspecies, and races, all of which aid in setting species recovery priorities. Each technique can be powerful in its own right but is most credible when used in conjunction with other molecular techniques and, most importantly, with ecological and demographic data collected from the field. Surprisingly few taxa of concern have been assayed with any molecular technique. Thus, rather than showcasing exhaustive details from a few well-known examples, this paper attempts to present a broad range of cases in which molecular techniques have been used to provide insight into conservation efforts.

Population genetic structure of endangered Mongolian racerunner (Eremias argus) from the Korean Peninsula.

PubMed

Park, Han-Chan; Suk, Ho Young; Jeong, Eu-Jin; Park, Dae-Sik; Lee, Hang; Min, Mi-Sook

2014-11-01

The Mongolian racerunner (Eremias argus) is a small lacertid lizard species, and its distribution range encompasses the Korean Peninsula, Mongolia, China and Russia. Eremias argus is widespread, but populations on the Korean Peninsula are small and declining, provoking concerns that genetic diversity is being lost. This species is currently listed under the Protection of Wild Fauna and Flora Act in South Korea. In this study, nine novel microsatellites for E. argus were developed with a biotin-enrichment method and used to understand its population genetic structure and delineate conservation units on the Korean Peninsula. Overall, low intrapopulation genetic diversity was observed (mean number of alleles per locus = 2.463; mean H E = 0.398) from 10 populations investigated (n = 110). Two populations (among five with n≥ 10) showed an excess of heterozygosity expected under HWE relative to that expected at mutation-drift equilibrium, indicating severe reduction in population sizes. With only a few exceptions, the overall genetic differentiation among populations was substantial with the high levels of pairwise-F ST (0.006-0.746) and -R ST (0.034-0.940) values. The results of Bayesian STRUCTURE analysis showed that E. argus populations on the Korean Peninsula were most likely partitioned into three genetic clusters. Taken all together, such low levels of gene flow and strong genetic structuring have critical implications for the conservation of this endangered species and its management.

Comparison of mitochondrial DNA control region sequence and microsatellite DNA analyses in estimating population structure and gene flow rates in Atlantic sturgeon Acipenser oxyrinchus

USGS Publications Warehouse

Wirgin, I.; Waldman, J.; Stabile, J.; Lubinski, B.; King, T.

2002-01-01

Atlantic sturgeon Acipenser oxyrinchus is large, long-lived, and anadromous with subspecies distributed along the Atlantic (A. oxyrinchus oxyrinchus) and Gulf of Mexico (A. o. desotoi) coasts of North America. Although it is not certain if extirpation of some population units has occurred, because of anthropogenic influences abundances of all populations are low compared with historical levels. Informed management of A. oxyrinchus demands a detailed knowledge of its population structure, levels of genetic diversity, and likelihood to home to natal rivers. We compared the use of mitochondrial DNA (mtDNA) control region sequence and microsatellite nuclear DNA (nDNA) analyses in identifying the stock structure and homing fidelity of Atlantic and Gulf coast populations of A. oxyrinchus. The approaches were concordant in that they revealed moderate to high levels of genetic diversity and suggested that populations of Atlantic sturgeon are highly structured. At least six genetically distinct management units were detected using the two approaches among the rivers surveyed. Mitochondrial DNA sequences revealed a significant cline in haplotype diversity along the Atlantic coast with monomorphism observed in Canadian populations. High levels of nDNA diversity were also observed among populations along the Atlantic coast, including the two Canadian populations, probably resulting from the more rapid rate of mutational and evolutionary change at microsatellite loci. Estimates of gene flow among populations were similar between both approaches with the exception that because of mtDNA monomorphism in Canadian populations, gene flow estimates between them were unobtainable. Analyses of both genomes provided high resolution and confidence in characterizing the population structure of Atlantic sturgeon. Microsatellite analysis was particularly informative in delineating population structure in rivers that were recently glaciated and may prove diagnostic in rivers that are geographically proximal along the south Atlantic coast of the US.

Assessing the viability of situationally driven segmentation opportunities in the health care market.

PubMed

Gehrt, K C; Pinto, M B

1993-01-01

The impact of situational factors has typically been investigated in the context of goods marketing. Very few studies have investigated the influence of situational factors on services marketing. This study demonstrates the importance of situational influence on services marketing by delineating a consumer-based, situationally characterized competitive market structure for health care services. The competitive structure of the health care market is delineated in terms of the similarity/substitutability of the three-factor, situational characterizations of ten health care alternatives. The general marketing implications of the market-structure delineation procedure and the health care-specific implications of the findings are discussed.

Genomic Rearrangements in Arabidopsis Considered as Quantitative Traits.

PubMed

Imprialou, Martha; Kahles, André; Steffen, Joshua G; Osborne, Edward J; Gan, Xiangchao; Lempe, Janne; Bhomra, Amarjit; Belfield, Eric; Visscher, Anne; Greenhalgh, Robert; Harberd, Nicholas P; Goram, Richard; Hein, Jotun; Robert-Seilaniantz, Alexandre; Jones, Jonathan; Stegle, Oliver; Kover, Paula; Tsiantis, Miltos; Nordborg, Magnus; Rätsch, Gunnar; Clark, Richard M; Mott, Richard

2017-04-01

To understand the population genetics of structural variants and their effects on phenotypes, we developed an approach to mapping structural variants that segregate in a population sequenced at low coverage. We avoid calling structural variants directly. Instead, the evidence for a potential structural variant at a locus is indicated by variation in the counts of short-reads that map anomalously to that locus. These structural variant traits are treated as quantitative traits and mapped genetically, analogously to a gene expression study. Association between a structural variant trait at one locus, and genotypes at a distant locus indicate the origin and target of a transposition. Using ultra-low-coverage (0.3×) population sequence data from 488 recombinant inbred Arabidopsis thaliana genomes, we identified 6502 segregating structural variants. Remarkably, 25% of these were transpositions. While many structural variants cannot be delineated precisely, we validated 83% of 44 predicted transposition breakpoints by polymerase chain reaction. We show that specific structural variants may be causative for quantitative trait loci for germination and resistance to infection by the fungus Albugo laibachii , isolate Nc14. Further we show that the phenotypic heritability attributable to read-mapping anomalies differs from, and, in the case of time to germination and bolting, exceeds that due to standard genetic variation. Genes within structural variants are also more likely to be silenced or dysregulated. This approach complements the prevalent strategy of structural variant discovery in fewer individuals sequenced at high coverage. It is generally applicable to large populations sequenced at low-coverage, and is particularly suited to mapping transpositions. Copyright © 2017 by the Genetics Society of America.

The roles of calving migration and climate change in the formation of the weak genetic structure in the Tibetan antelope (Pantholops hodgsonii).

PubMed

Chen, Jiarui; Lin, Gonghua; Qin, Wen; Yan, Jingyan; Zhang, Tongzuo; Su, Jianping

2018-05-31

Geographical barriers and distance can reduce gene exchange among animals, resulting in genetic divergence of geographically isolated populations. The habitats of Tibetan antelope (Pantholops hodgsonii) has a geographical range of approximately 1,600 km across the Qinghai-Tibet Plateau (QTP) with a series tall mountains and big rivers. However, previously studies indicated that there was little genetic differentiation among their geographically delineated populations. To better understand the genetic structure of P. hodgsonii populations, we collected 145 samples from the three major calving regions considering their various calving grounds and migration routes. We used a combination of mitochondrial sequences (Cyt b, ATPase, D-loop and COX I) to investigate the genetic structure and the evolutionary divergence of the populations. Significant, albeit weak, genetic differentiation was detected among the three geographical populations. Analysis of the genetic divergence process revealed that the animals gradually entered into a period of rapid genetic differentiation since approximately 60,000 years ago. The calving migration of P. hodgsonii cannot be the main cause of their weak genetic structure since such cannot fully homogenize the genetic pool. Instead, the geological and climatic events as well as the coupling vegetation succession process during this period have been suggested to greatly contribute to the genetic structure and the expansion of genetic diversity. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

Population Genetic Structure of the Tropical Two-Wing Flyingfish (Exocoetus volitans)

PubMed Central

Lewallen, Eric A.; Bohonak, Andrew J.; Bonin, Carolina A.; van Wijnen, Andre J.; Pitman, Robert L.; Lovejoy, Nathan R.

2016-01-01

Delineating populations of pantropical marine fish is a difficult process, due to widespread geographic ranges and complex life history traits in most species. Exocoetus volitans, a species of two-winged flyingfish, is a good model for understanding large-scale patterns of epipelagic fish population structure because it has a circumtropical geographic range and completes its entire life cycle in the epipelagic zone. Buoyant pelagic eggs should dictate high local dispersal capacity in this species, although a brief larval phase, small body size, and short lifespan may limit the dispersal of individuals over large spatial scales. Based on these biological features, we hypothesized that E. volitans would exhibit statistically and biologically significant population structure defined by recognized oceanographic barriers. We tested this hypothesis by analyzing cytochrome b mtDNA sequence data (1106 bps) from specimens collected in the Pacific, Atlantic and Indian oceans (n = 266). AMOVA, Bayesian, and coalescent analytical approaches were used to assess and interpret population-level genetic variability. A parsimony-based haplotype network did not reveal population subdivision among ocean basins, but AMOVA revealed limited, statistically significant population structure between the Pacific and Atlantic Oceans (ΦST = 0.035, p<0.001). A spatially-unbiased Bayesian approach identified two circumtropical population clusters north and south of the Equator (ΦST = 0.026, p<0.001), a previously unknown dispersal barrier for an epipelagic fish. Bayesian demographic modeling suggested the effective population size of this species increased by at least an order of magnitude ~150,000 years ago, to more than 1 billion individuals currently. Thus, high levels of genetic similarity observed in E. volitans can be explained by high rates of gene flow, a dramatic and recent population expansion, as well as extensive and consistent dispersal throughout the geographic range of the species. PMID:27736863

Population Genetic Structure of the Tropical Two-Wing Flyingfish (Exocoetus volitans).

PubMed

Lewallen, Eric A; Bohonak, Andrew J; Bonin, Carolina A; van Wijnen, Andre J; Pitman, Robert L; Lovejoy, Nathan R

2016-01-01

Delineating populations of pantropical marine fish is a difficult process, due to widespread geographic ranges and complex life history traits in most species. Exocoetus volitans, a species of two-winged flyingfish, is a good model for understanding large-scale patterns of epipelagic fish population structure because it has a circumtropical geographic range and completes its entire life cycle in the epipelagic zone. Buoyant pelagic eggs should dictate high local dispersal capacity in this species, although a brief larval phase, small body size, and short lifespan may limit the dispersal of individuals over large spatial scales. Based on these biological features, we hypothesized that E. volitans would exhibit statistically and biologically significant population structure defined by recognized oceanographic barriers. We tested this hypothesis by analyzing cytochrome b mtDNA sequence data (1106 bps) from specimens collected in the Pacific, Atlantic and Indian oceans (n = 266). AMOVA, Bayesian, and coalescent analytical approaches were used to assess and interpret population-level genetic variability. A parsimony-based haplotype network did not reveal population subdivision among ocean basins, but AMOVA revealed limited, statistically significant population structure between the Pacific and Atlantic Oceans (ΦST = 0.035, p<0.001). A spatially-unbiased Bayesian approach identified two circumtropical population clusters north and south of the Equator (ΦST = 0.026, p<0.001), a previously unknown dispersal barrier for an epipelagic fish. Bayesian demographic modeling suggested the effective population size of this species increased by at least an order of magnitude ~150,000 years ago, to more than 1 billion individuals currently. Thus, high levels of genetic similarity observed in E. volitans can be explained by high rates of gene flow, a dramatic and recent population expansion, as well as extensive and consistent dispersal throughout the geographic range of the species.

Incorporating priors on expert performance parameters for segmentation validation and label fusion: a maximum a posteriori STAPLE

PubMed Central

Commowick, Olivier; Warfield, Simon K

2010-01-01

In order to evaluate the quality of segmentations of an image and assess intra- and inter-expert variability in segmentation performance, an Expectation Maximization (EM) algorithm for Simultaneous Truth And Performance Level Estimation (STAPLE) was recently developed. This algorithm, originally presented for segmentation validation, has since been used for many applications, such as atlas construction and decision fusion. However, the manual delineation of structures of interest is a very time consuming and burdensome task. Further, as the time required and burden of manual delineation increase, the accuracy of the delineation is decreased. Therefore, it may be desirable to ask the experts to delineate only a reduced number of structures or the segmentation of all structures by all experts may simply not be achieved. Fusion from data with some structures not segmented by each expert should be carried out in a manner that accounts for the missing information. In other applications, locally inconsistent segmentations may drive the STAPLE algorithm into an undesirable local optimum, leading to misclassifications or misleading experts performance parameters. We present a new algorithm that allows fusion with partial delineation and which can avoid convergence to undesirable local optima in the presence of strongly inconsistent segmentations. The algorithm extends STAPLE by incorporating prior probabilities for the expert performance parameters. This is achieved through a Maximum A Posteriori formulation, where the prior probabilities for the performance parameters are modeled by a beta distribution. We demonstrate that this new algorithm enables dramatically improved fusion from data with partial delineation by each expert in comparison to fusion with STAPLE. PMID:20879379

Incorporating priors on expert performance parameters for segmentation validation and label fusion: a maximum a posteriori STAPLE.

PubMed

Commowick, Olivier; Warfield, Simon K

2010-01-01

In order to evaluate the quality of segmentations of an image and assess intra- and inter-expert variability in segmentation performance, an Expectation Maximization (EM) algorithm for Simultaneous Truth And Performance Level Estimation (STAPLE) was recently developed. This algorithm, originally presented for segmentation validation, has since been used for many applications, such as atlas construction and decision fusion. However, the manual delineation of structures of interest is a very time consuming and burdensome task. Further, as the time required and burden of manual delineation increase, the accuracy of the delineation is decreased. Therefore, it may be desirable to ask the experts to delineate only a reduced number of structures or the segmentation of all structures by all experts may simply not be achieved. Fusion from data with some structures not segmented by each expert should be carried out in a manner that accounts for the missing information. In other applications, locally inconsistent segmentations may drive the STAPLE algorithm into an undesirable local optimum, leading to misclassifications or misleading experts performance parameters. We present a new algorithm that allows fusion with partial delineation and which can avoid convergence to undesirable local optima in the presence of strongly inconsistent segmentations. The algorithm extends STAPLE by incorporating prior probabilities for the expert performance parameters. This is achieved through a Maximum A Posteriori formulation, where the prior probabilities for the performance parameters are modeled by a beta distribution. We demonstrate that this new algorithm enables dramatically improved fusion from data with partial delineation by each expert in comparison to fusion with STAPLE.

Variation in MHC class II B genes in marbled murrelets: implications for delineating conservation units

Treesearch

C. Vásquez-Carrillo; V. Friesen; L. Hall; M.Z. Peery

2013-01-01

Conserving genetic variation is critical for maintaining the evolutionary potential and viability of a species. Genetic studies seeking to delineate conservation units, however, typically focus on characterizing neutral genetic variation and may not identify populations harboring local adaptations. Here, variation at two major histocompatibility complex (MHC) class II...

Ethnicity and Population Structure in Personal Naming Networks

PubMed Central

Mateos, Pablo; Longley, Paul A.; O'Sullivan, David

2011-01-01

Personal naming practices exist in all human groups and are far from random. Rather, they continue to reflect social norms and ethno-cultural customs that have developed over generations. As a consequence, contemporary name frequency distributions retain distinct geographic, social and ethno-cultural patterning that can be exploited to understand population structure in human biology, public health and social science. Previous attempts to detect and delineate such structure in large populations have entailed extensive empirical analysis of naming conventions in different parts of the world without seeking any general or automated methods of population classification by ethno-cultural origin. Here we show how ‘naming networks’, constructed from forename-surname pairs of a large sample of the contemporary human population in 17 countries, provide a valuable representation of cultural, ethnic and linguistic population structure around the world. This innovative approach enriches and adds value to automated population classification through conventional national data sources such as telephone directories and electoral registers. The method identifies clear social and ethno-cultural clusters in such naming networks that extend far beyond the geographic areas in which particular names originated, and that are preserved even after international migration. Moreover, one of the most striking findings of this approach is that these clusters simply ‘emerge’ from the aggregation of millions of individual decisions on parental naming practices for their children, without any prior knowledge introduced by the researcher. Our probabilistic approach to community assignment, both at city level as well as at a global scale, helps to reveal the degree of isolation, integration or overlap between human populations in our rapidly globalising world. As such, this work has important implications for research in population genetics, public health, and social science adding new understandings of migration, identity, integration and social interaction across the world. PMID:21909399

Distance, dams and drift: What structures populations of an endangered, benthic stream fish?

USGS Publications Warehouse

Roberts, James H.; Angermeier, Paul; Hallerman, Eric M.

2013-01-01

Spatial population structure plays an important role in species persistence, evolution and conservation. Benthic stream fishes are diverse and frequently imperilled, yet the determinants and spatial scaling of their population structure are understudied. We investigated the range-wide population genetic structure of Roanoke logperch (Percina rex), an endangered, benthic stream fish of the eastern United States. Fish were sampled from 35 sites and analysed at 11 microsatellite DNA loci. Clustering models were used to sort individuals into genetically cohesive groups and thereby estimate the spatial scaling of population structure. We then used Bayesian generalized linear mixed models (BGLMMs) to test alternative hypotheses about the environmental factors most responsible for generating structure, as measured by the differentiation statistic FST. Clustering models delineated seven discrete populations, whose boundaries coincided with agents of fragmentation, including hydroelectric dams and tailwaters. In the absence of hydrological barriers, gene flow was extensive throughout catchments, whereas there was no evidence for contemporary dispersal between catchments across barriers. In the best-supported BGLMM, FST was positively related to the spatial distance and degree of hydrological alteration between sites and negatively related to genetic diversity within sites. Whereas the effect of tailwaters was equivocal, dams strongly influenced differentiation: the effect of a dam on FST was comparable to that of a between-site distance of over 1200 km of unimpounded river. Overall, the effect of distance-mediated dispersal was negligible compared to the combined effects of fragmentation and genetic drift. The contemporary population structure of P. rex comprises a few geographically extensive ‘islands’ that are fragmented by hydroelectric projects. This information clarifies the importance of a catchment-scale perspective on conserving the species and suggests that its recovery may require genetic and/or demographic reconnection of presently isolated populations.

Cultural sensitivity in public health: defined and demystified.

PubMed

Resnicow, K; Baranowski, T; Ahluwalia, J S; Braithwaite, R L

1999-01-01

There is consensus that health promotion programs should be culturally sensitive (CS). Yet, despite the ubiquitous nature of CS within public health research and practice, there has been surprisingly little attention given to defining CS or delineating a framework for developing culturally sensitive programs and practitioners. This paper describes a model for understanding CS from a public health perspective; describes a process for applying this model in the development of health promotion and disease prevention interventions; and highlights research priorities. Cultural sensitivity is defined by two dimensions: surface and deep structures. Surface structure involves matching intervention materials and messages to observable, "superficial" characteristics of a target population. This may involve using people, places, language, music, food, locations, and clothing familiar to, and preferred by, the target audience. Surface structure refers to how well interventions fit within a specific culture. Deep structure involves incorporating the cultural, social, historical, environmental and psychological forces that influence the target health behavior in the proposed target population. Whereas surface structure generally increases the "receptivity" or "acceptance" of messages, deep structure conveys salience. Techniques, borrowed from social marketing and health communication theory, for developing culturally sensitive interventions are described. Research is needed to determine the effectiveness of culturally sensitive programs.

A patient with myelodysplastic syndrome studied by GTG-banding and fluorescent in situ hybridization (FISH).

PubMed

Mark, H F; Mark, Y; Sotomayor, E; Sambandam, S

1998-01-01

Molecular cytogenetics using fluorescent in situ hybridization (FISH) is an extremely useful adjunct technique to conventional cytogenetics via GTG-banding. The present paper illustrates the utility of FISH by describing a patient with myelodysplastic syndrome (MDS) who was initially studied using GTG-banding and whose bone marrow was found to be populated with hyperdiploid cells. FISH was used to delineate the numerical and structural chromosomal abnormalities. It revealed the presence of trisomy 8 and determined that the previously unidentifiable marker chromosome was of chromosome 10 origin. Although trisomy 8 is a frequent finding in MDS, the structural chromosomal abnormality of chromosome 10 as reported here is not a common finding.

Efficacy of computerized discrimination between structure-related and non-structure-related echoes in ultrasonographic images for the quantitative evaluation of the structural integrity of superficial digital flexor tendons in horses.

PubMed

van Schie, H T; Bakker, E M; Jonker, A M; van Weeren, P R

2001-07-01

To evaluate effectiveness of computerized discrimination between structure-related and non-structure-related echoes in ultrasonographic images for quantitative evaluation of tendon structural integrity in horses. 4 superficial digital flexor tendons (2 damaged tendons, 2 normal tendons). Transverse ultrasonographic images that precisely matched histologic sections were obtained in fixed steps along the long axis of each tendon. Distribution, intensity, and delineation of structure-related echoes, quantitatively expressed as the correlation ratio and steadiness ratio , were compared with histologic findings in tissue that was normal or had necrosis, early granulation, late granulation, early fibrosis, or inferior repair. In normal tendon, the even distribution of structure-related echoes with high intensity and sharp delineation yielded high correlation ratio and steadiness ratio. In areas of necrosis, collapsed endotendon septa yielded solid but blurred structure-related echoes (high correlation ration and low steadiness ratio). In early granulation tissue, complete lack of organization caused zero values for both ratios. In late granulation tissue, reorganization and swollen endotendon septa yielded poorly delineated structure-related echoes (high correlation ratio, low steadiness ratio). In early fibrosis, rearrangement of bundles resulted in normal correlation ration and slightly low steadiness ratio. In inferior repair, the almost complete lack of structural reorganization resulted in heterogeneous poorly delineated low-intensity echoes (low correlation ratio and steadiness ratio). The combination of correlation ratio and steadiness ratio accurately reflects histopathologic findings, making computerized correlation of ultrasonographic images an efficient tool for quantitative evaluation of tendon structural integrity.

Applications of graph theory to landscape genetics

PubMed Central

Garroway, Colin J; Bowman, Jeff; Carr, Denis; Wilson, Paul J

2008-01-01

We investigated the relationships among landscape quality, gene flow, and population genetic structure of fishers (Martes pennanti) in ON, Canada. We used graph theory as an analytical framework considering each landscape as a network node. The 34 nodes were connected by 93 edges. Network structure was characterized by a higher level of clustering than expected by chance, a short mean path length connecting all pairs of nodes, and a resiliency to the loss of highly connected nodes. This suggests that alleles can be efficiently spread through the system and that extirpations and conservative harvest are not likely to affect their spread. Two measures of node centrality were negatively related to both the proportion of immigrants in a node and node snow depth. This suggests that central nodes are producers of emigrants, contain high-quality habitat (i.e., deep snow can make locomotion energetically costly) and that fishers were migrating from high to low quality habitat. A method of community detection on networks delineated five genetic clusters of nodes suggesting cryptic population structure. Our analyses showed that network models can provide system-level insight into the process of gene flow with implications for understanding how landscape alterations might affect population fitness and evolutionary potential. PMID:25567802

Using Vocal Dialects to Assess the Population Structure of Bigg's Killer Whales in Alaska

NASA Astrophysics Data System (ADS)

Sharpe, D. L.; Wade, P. R.; Castellote, M.; Cornick, L. A.

2016-02-01

Apex predators are important indicators of ecosystem health, but little is known about the population structure of Bigg's killer whales (Orcinus orca; i.e. "transient" ecotype) in western Alaska. Currently, all Bigg's killer whales in western Alaska are ascribed to a single broad stock for management under the US Marine Mammal Protection Act. However, recent nuclear microsatellite and mitochondrial DNA analyses indicate that this stock is likely comprised of genetically distinct sub-populations. In accordance with what is known about group-specific killer whale vocal dialects in other locations, we sought to evaluate and refine Bigg's killer whale population structure by using acoustic recordings to examine the spatial distribution of call types in western Alaska. Digital audio recordings were collected from 34 encounters with Bigg's killer whales throughout the Aleutian and Pribilof Islands in the summers of 2001-2007 and 2009-2010, then visually and aurally reviewed using the software Adobe Audition. High quality calls were identified and classified into discrete call types based on spectrographic characteristics and aural uniqueness. A comparative analysis of call types recorded throughout the study area revealed spatial segregation of call types, corresponding well with proposed genetic delineations. These results suggest that Bigg's killer whales exhibit regional vocal dialects, which can be used to help refine the putative sub-populations that have been genetically identified throughout western Alaska. Our findings support the proposal to restructure current stock designations.

Comparison of U-spatial statistics and C-A fractal models for delineating anomaly patterns of porphyry-type Cu geochemical signatures in the Varzaghan district, NW Iran

NASA Astrophysics Data System (ADS)

Ghezelbash, Reza; Maghsoudi, Abbas

2018-05-01

The delineation of populations of stream sediment geochemical data is a crucial task in regional exploration surveys. In this contribution, uni-element stream sediment geochemical data of Cu, Au, Mo, and Bi have been subjected to two reliable anomaly-background separation methods, namely, the concentration-area (C-A) fractal and the U-spatial statistics methods to separate geochemical anomalies related to porphyry-type Cu mineralization in northwest Iran. The quantitative comparison of the delineated geochemical populations using the modified success-rate curves revealed the superiority of the U-spatial statistics method over the fractal model. Moreover, geochemical maps of investigated elements revealed strongly positive correlations between strong anomalies and Oligocene-Miocene intrusions in the study area. Therefore, follow-up exploration programs should focus on these areas.

Object-oriented classification of forest structure from light detection and ranging data for stand mapping

Treesearch

Alicia A. Sullivan; Robert J. McGaughey; Hans-Erik Andersen; Peter Schiess

2009-01-01

Stand delineation is an important step in the process of establishing a forest inventory and provides the spatial framework for many forest management decisions. Many methods for extracting forest structure characteristics for stand delineation and other purposes have been researched in the past, primarily focusing on high-resolution imagery and satellite data. High-...

Phenotypic and genetic structure of traits delineating personality disorder.

PubMed

Livesley, W J; Jang, K L; Vernon, P A

1998-10-01

The evidence suggests that personality traits are hierarchically organized with more specific or lower-order traits combining to form more generalized higher-order traits. Agreement exists across studies regarding the lower-order traits that delineate personality disorder but not the higher-order traits. This study seeks to identify the higher-order structure of personality disorder by examining the phenotypic and genetic structures underlying lower-order traits. Eighteen lower-order traits were assessed using the Dimensional Assessment of Personality Disorder-Basic Questionnaire in samples of 656 personality disordered patients, 939 general population subjects, and a volunteer sample of 686 twin pairs. Principal components analysis yielded 4 components, labeled Emotional Dysregulation, Dissocial Behavior, Inhibitedness, and Compulsivity, that were similar across the 3 samples. Multivariate genetic analyses also yielded 4 genetic and environmental factors that were remarkably similar to the phenotypic factors. Analysis of the residual heritability of the lower-order traits when the effects of the higher-order factors were removed revealed a substantial residual heritable component for 12 of the 18 traits. The results support the following conclusions. First, the stable structure of traits across clinical and nonclinical samples is consistent with dimensional representations of personality disorders. Second, the higher-order traits of personality disorder strongly resemble dimensions of normal personality. This implies that a dimensional classification should be compatible with normative personality. Third, the residual heritability of the lower-order traits suggests that the personality phenotypes are based on a large number of specific genetic components.

Cytoplasmic DNA variation and biogeography of Larix Mill. in northeast Asia.

PubMed

Polezhaeva, Maria A; Lascoux, Martin; Semerikov, Vladimir L

2010-03-01

Range-wide variation in 54 populations of Dahurian larch (Larix gmelinii) and related taxa in Northeast Asia was assessed with four mitochondrial PCR-RFLP and five chloroplast SSR markers. Eleven mitotypes and 115 chlorotypes were detected. The highest diversity was observed in the southern Russian Far East where hybrids of L. gmelinii, L. olgensis and L. kamtschatica are distributed. In contrast, only two mitotypes occurred in L. cajanderi and L. gmelinii. The Japanese larch (L. kaempferi) was found to be closely related to populations of L. kamtschatica inhabiting the Kuril Islands and South Sakhalin, populations from the northern part of Sakhalin being more closely related to continental species. In general, both mitochondrial (G(ST) = 0.786; N(ST) = 0.823) and chloroplast (G(ST) = 0.144; R(ST) = 0.432) markers showed a strong phylogeographical structure and evidence of isolation-by-distance. Yet both markers did not allow a clear delineation of species borders. In particular, and contrary to expectations, cpDNA was not significantly better than mtDNA at delineating species borders. This lack of concordance between morphological species and molecular markers could reflect extensive ancestral haplotype sharing and past and ongoing introgression. Finally the distribution of mtDNA and cpDNA variation suggests the presence of several refugia during Pleistocene glacial intervals. In particular, mtDNA and cpDNA reveal weak but visible differentiation between L. gmelinii and L. cajanderi, suggesting independent glacial histories of these species.

Assessing Genetic Structure in Common but Ecologically Distinct Carnivores: The Stone Marten and Red Fox.

PubMed

Basto, Mafalda P; Santos-Reis, Margarida; Simões, Luciana; Grilo, Clara; Cardoso, Luís; Cortes, Helder; Bruford, Michael W; Fernandes, Carlos

2016-01-01

The identification of populations and spatial genetic patterns is important for ecological and conservation research, and spatially explicit individual-based methods have been recognised as powerful tools in this context. Mammalian carnivores are intrinsically vulnerable to habitat fragmentation but not much is known about the genetic consequences of fragmentation in common species. Stone martens (Martes foina) and red foxes (Vulpes vulpes) share a widespread Palearctic distribution and are considered habitat generalists, but in the Iberian Peninsula stone martens tend to occur in higher quality habitats. We compared their genetic structure in Portugal to see if they are consistent with their differences in ecological plasticity, and also to illustrate an approach to explicitly delineate the spatial boundaries of consistently identified genetic units. We analysed microsatellite data using spatial Bayesian clustering methods (implemented in the software BAPS, GENELAND and TESS), a progressive partitioning approach and a multivariate technique (Spatial Principal Components Analysis-sPCA). Three consensus Bayesian clusters were identified for the stone marten. No consensus was achieved for the red fox, but one cluster was the most probable clustering solution. Progressive partitioning and sPCA suggested additional clusters in the stone marten but they were not consistent among methods and were geographically incoherent. The contrasting results between the two species are consistent with the literature reporting stricter ecological requirements of the stone marten in the Iberian Peninsula. The observed genetic structure in the stone marten may have been influenced by landscape features, particularly rivers, and fragmentation. We suggest that an approach based on a consensus clustering solution of multiple different algorithms may provide an objective and effective means to delineate potential boundaries of inferred subpopulations. sPCA and progressive partitioning offer further verification of possible population structure and may be useful for revealing cryptic spatial genetic patterns worth further investigation.

Assessing Genetic Structure in Common but Ecologically Distinct Carnivores: The Stone Marten and Red Fox

PubMed Central

Basto, Mafalda P.; Santos-Reis, Margarida; Simões, Luciana; Grilo, Clara; Cardoso, Luís; Cortes, Helder; Bruford, Michael W.; Fernandes, Carlos

2016-01-01

The identification of populations and spatial genetic patterns is important for ecological and conservation research, and spatially explicit individual-based methods have been recognised as powerful tools in this context. Mammalian carnivores are intrinsically vulnerable to habitat fragmentation but not much is known about the genetic consequences of fragmentation in common species. Stone martens (Martes foina) and red foxes (Vulpes vulpes) share a widespread Palearctic distribution and are considered habitat generalists, but in the Iberian Peninsula stone martens tend to occur in higher quality habitats. We compared their genetic structure in Portugal to see if they are consistent with their differences in ecological plasticity, and also to illustrate an approach to explicitly delineate the spatial boundaries of consistently identified genetic units. We analysed microsatellite data using spatial Bayesian clustering methods (implemented in the software BAPS, GENELAND and TESS), a progressive partitioning approach and a multivariate technique (Spatial Principal Components Analysis-sPCA). Three consensus Bayesian clusters were identified for the stone marten. No consensus was achieved for the red fox, but one cluster was the most probable clustering solution. Progressive partitioning and sPCA suggested additional clusters in the stone marten but they were not consistent among methods and were geographically incoherent. The contrasting results between the two species are consistent with the literature reporting stricter ecological requirements of the stone marten in the Iberian Peninsula. The observed genetic structure in the stone marten may have been influenced by landscape features, particularly rivers, and fragmentation. We suggest that an approach based on a consensus clustering solution of multiple different algorithms may provide an objective and effective means to delineate potential boundaries of inferred subpopulations. sPCA and progressive partitioning offer further verification of possible population structure and may be useful for revealing cryptic spatial genetic patterns worth further investigation. PMID:26727497

Microsatellite DNA markers for delineating population structure and kinship among the endangered Kirtland's warbler (Dendroica kirtlandii)

USGS Publications Warehouse

King, T.L.; Eackles, M.S.; Henderson, A.P.; Bocetti, Carol I.; Currie, D.; Wunderle, J.M.

2005-01-01

We document the isolation and characterization of 23 microsatellite DNA markers for the endangered Kirtland's warbler (Dendroica kirtlandii), a Nearctic/Neotropical migrant passerine. This suite of markers revealed moderate to high levels of allelic diversity (averaging 7.7 alleles per locus) and heterozygosity (averaging 72%). Genotypic frequencies at 22 of 23 (95%) markers conformed to Hardy-Weinberg equilibrium expectations, and no linkage disequilibrium was observed in blood samples taken from 14 warblers found on the wintering grounds in the Bahamas archipelago. Multilocus genotypes resulting from this suite of markers should reduce the amount of resources required for initiating new genetic studies assessing breeding structure, parentage, demographics, and individual-level ecological interactions for D. kirtlandii. ?? 2005 Blackwell Publishing Ltd.

Map showing depth to bedrock of the Tacoma and part of the Centralia 30' x 60' quadrangles, Washington

USGS Publications Warehouse

Buchanan-Banks, Jane M.; Collins, Donley S.

1994-01-01

The heavily populated Puget Sound region in the State of Washington has experienced moderate to large earthquakes in the recent past (Nuttli, 1952; Mullineaux and others, 1967). Maps showing thickness of unconsolidated sedimentary deposits are useful aids in delineating areas where damage to engineered structures can result from increased shaking resulting from these earthquakes. Basins containing thick deposits of unconsolidated materials can amplify earthquakes waves and cause far more damage to structures than the same waves passing through bedrock (Singh and others, 1988; Algermissen and others, 1985). Configurations of deep sedimentary basins can also cause reflection and magnification of earthquake waves in ways still not fully understood and presently under investigation (Frankel and Vidale, 1992).

Spatially Explicit Analysis of Genome-Wide SNPs Detects Subtle Population Structure in a Mobile Marine Mammal, the Harbor Porpoise

PubMed Central

Lah, Ljerka; Trense, Daronja; Benke, Harald; Berggren, Per; Gunnlaugsson, Þorvaldur; Lockyer, Christina; Öztürk, Ayaka; Öztürk, Bayram; Pawliczka, Iwona; Roos, Anna; Siebert, Ursula; Víkingsson, Gísli; Tiedemann, Ralph

2016-01-01

The population structure of the highly mobile marine mammal, the harbor porpoise (Phocoena phocoena), in the Atlantic shelf waters follows a pattern of significant isolation-by-distance. The population structure of harbor porpoises from the Baltic Sea, which is connected with the North Sea through a series of basins separated by shallow underwater ridges, however, is more complex. Here, we investigated the population differentiation of harbor porpoises in European Seas with a special focus on the Baltic Sea and adjacent waters, using a population genomics approach. We used 2872 single nucleotide polymorphisms (SNPs), derived from double digest restriction-site associated DNA sequencing (ddRAD-seq), as well as 13 microsatellite loci and mitochondrial haplotypes for the same set of individuals. Spatial principal components analysis (sPCA), and Bayesian clustering on a subset of SNPs suggest three main groupings at the level of all studied regions: the Black Sea, the North Atlantic, and the Baltic Sea. Furthermore, we observed a distinct separation of the North Sea harbor porpoises from the Baltic Sea populations, and identified splits between porpoise populations within the Baltic Sea. We observed a notable distinction between the Belt Sea and the Inner Baltic Sea sub-regions. Improved delineation of harbor porpoise population assignments for the Baltic based on genomic evidence is important for conservation management of this endangered cetacean in threatened habitats, particularly in the Baltic Sea proper. In addition, we show that SNPs outperform microsatellite markers and demonstrate the utility of RAD-tags from a relatively small, opportunistically sampled cetacean sample set for population diversity and divergence analysis. PMID:27783621

Modeling ecological minimum requirements for distribution of greater sage-grouse leks: implications for population connectivity across their western range, U.S.A.

PubMed

Knick, Steven T; Hanser, Steven E; Preston, Kristine L

2013-06-01

Greater sage-grouse Centrocercus urophasianus (Bonaparte) currently occupy approximately half of their historical distribution across western North America. Sage-grouse are a candidate for endangered species listing due to habitat and population fragmentation coupled with inadequate regulation to control development in critical areas. Conservation planning would benefit from accurate maps delineating required habitats and movement corridors. However, developing a species distribution model that incorporates the diversity of habitats used by sage-grouse across their widespread distribution has statistical and logistical challenges. We first identified the ecological minimums limiting sage-grouse, mapped similarity to the multivariate set of minimums, and delineated connectivity across a 920,000 km(2) region. We partitioned a Mahalanobis D (2) model of habitat use into k separate additive components each representing independent combinations of species-habitat relationships to identify the ecological minimums required by sage-grouse. We constructed the model from abiotic, land cover, and anthropogenic variables measured at leks (breeding) and surrounding areas within 5 km. We evaluated model partitions using a random subset of leks and historic locations and selected D (2) (k = 10) for mapping a habitat similarity index (HSI). Finally, we delineated connectivity by converting the mapped HSI to a resistance surface. Sage-grouse required sagebrush-dominated landscapes containing minimal levels of human land use. Sage-grouse used relatively arid regions characterized by shallow slopes, even terrain, and low amounts of forest, grassland, and agriculture in the surrounding landscape. Most populations were interconnected although several outlying populations were isolated because of distance or lack of habitat corridors for exchange. Land management agencies currently are revising land-use plans and designating critical habitat to conserve sage-grouse and avoid endangered species listing. Our results identifying attributes important for delineating habitats or modeling connectivity will facilitate conservation and management of landscapes important for supporting current and future sage-grouse populations.

Modeling ecological minimum requirements for distribution of greater sage-grouse leks: implications for population connectivity across their western range, U.S.A.

USGS Publications Warehouse

Knick, Steven T.; Hanser, Steven E.; Preston, Kristine L.

2013-01-01

Greater sage-grouse Centrocercus urophasianus (Bonaparte) currently occupy approximately half of their historical distribution across western North America. Sage-grouse are a candidate for endangered species listing due to habitat and population fragmentation coupled with inadequate regulation to control development in critical areas. Conservation planning would benefit from accurate maps delineating required habitats and movement corridors. However, developing a species distribution model that incorporates the diversity of habitats used by sage-grouse across their widespread distribution has statistical and logistical challenges. We first identified the ecological minimums limiting sage-grouse, mapped similarity to the multivariate set of minimums, and delineated connectivity across a 920,000 km2 region. We partitioned a Mahalanobis D2 model of habitat use into k separate additive components each representing independent combinations of species–habitat relationships to identify the ecological minimums required by sage-grouse. We constructed the model from abiotic, land cover, and anthropogenic variables measured at leks (breeding) and surrounding areas within 5 km. We evaluated model partitions using a random subset of leks and historic locations and selected D2 (k = 10) for mapping a habitat similarity index (HSI). Finally, we delineated connectivity by converting the mapped HSI to a resistance surface. Sage-grouse required sagebrush-dominated landscapes containing minimal levels of human land use. Sage-grouse used relatively arid regions characterized by shallow slopes, even terrain, and low amounts of forest, grassland, and agriculture in the surrounding landscape. Most populations were interconnected although several outlying populations were isolated because of distance or lack of habitat corridors for exchange. Land management agencies currently are revising land-use plans and designating critical habitat to conserve sage-grouse and avoid endangered species listing. Our results identifying attributes important for delineating habitats or modeling connectivity will facilitate conservation and management of landscapes important for supporting current and future sage-grouse populations.

Call types of Bigg's killer whales (Orcinus orca) in western Alaska: Using vocal dialects to assess population structure

NASA Astrophysics Data System (ADS)

Sharpe, Deborah Lynn

Apex predators are important indicators of ecosystem health, but little is known about the population structure of Bigg's killer whales ( Orcinus orca; i.e. 'transient' ecotype) in western Alaska. Currently, all Bigg's killer whales in western Alaska are ascribed to a single broad stock for management under the US Marine Mammal Protection Act. However, recent nuclear microsatellite and mitochondrial DNA analyses indicate that this stock is likely comprised of genetically distinct sub-populations. In accordance with what is known about killer whale vocal dialects in other locations, I sought to evaluate Bigg's killer whale population structure by examining the spatial distribution of group-specific call types in western Alaska. Digital audio recordings were collected from 33 encounters with Bigg's killer whales throughout the Aleutian and Pribilof Islands in the summers of 2001-2007 and 2009-2010. Recorded calls were perceptually classified into discrete types and then quantitatively described using 12 structural and time-frequency measures. Resulting call categories were objectively validated using a random forest approach. A total of 36 call types and subtypes were identified across the entire study area, and regional patterns of call type usage revealed three distinct dialects, each of which corresponding to proposed genetic delineations. I suggest that at least three acoustically and genetically distinct subpopulations are present in western Alaska, and put forth an initial catalog for this area describing the regional vocal repertoires of Bigg's killer whale call types.

Strontium isotopes delineate fine-scale natal origins and migration histories of Pacific salmon

USGS Publications Warehouse

Brennan, Sean R.; Zimmerman, Christian E.; Fernandez, Diego P.; Cerling, Thure E.; McPhee, Megan V.; Wooller, Matthew J.

2015-01-01

Highly migratory organisms present major challenges to conservation efforts. This is especially true for exploited anadromous fish species, which exhibit long-range dispersals from natal sites, complex population structures, and extensive mixing of distinct populations during exploitation. By tracing the migratory histories of individual Chinook salmon caught in fisheries using strontium isotopes, we determined the relative production of natal habitats at fine spatial scales and different life histories. Although strontium isotopes have been widely used in provenance research, we present a new robust framework to simultaneously assess natal sources and migrations of individuals within fishery harvests through time. Our results pave the way for investigating how fine-scale habitat production and life histories of salmon respond to perturbations—providing crucial insights for conservation.

QSAR modeling and chemical space analysis of antimalarial compounds

NASA Astrophysics Data System (ADS)

Sidorov, Pavel; Viira, Birgit; Davioud-Charvet, Elisabeth; Maran, Uko; Marcou, Gilles; Horvath, Dragos; Varnek, Alexandre

2017-05-01

Generative topographic mapping (GTM) has been used to visualize and analyze the chemical space of antimalarial compounds as well as to build predictive models linking structure of molecules with their antimalarial activity. For this, a database, including 3000 molecules tested in one or several of 17 anti- Plasmodium activity assessment protocols, has been compiled by assembling experimental data from in-house and ChEMBL databases. GTM classification models built on subsets corresponding to individual bioassays perform similarly to the earlier reported SVM models. Zones preferentially populated by active and inactive molecules, respectively, clearly emerge in the class landscapes supported by the GTM model. Their analysis resulted in identification of privileged structural motifs of potential antimalarial compounds. Projection of marketed antimalarial drugs on this map allowed us to delineate several areas in the chemical space corresponding to different mechanisms of antimalarial activity. This helped us to make a suggestion about the mode of action of the molecules populating these zones.

QSAR modeling and chemical space analysis of antimalarial compounds.

PubMed

Sidorov, Pavel; Viira, Birgit; Davioud-Charvet, Elisabeth; Maran, Uko; Marcou, Gilles; Horvath, Dragos; Varnek, Alexandre

2017-05-01

Generative topographic mapping (GTM) has been used to visualize and analyze the chemical space of antimalarial compounds as well as to build predictive models linking structure of molecules with their antimalarial activity. For this, a database, including ~3000 molecules tested in one or several of 17 anti-Plasmodium activity assessment protocols, has been compiled by assembling experimental data from in-house and ChEMBL databases. GTM classification models built on subsets corresponding to individual bioassays perform similarly to the earlier reported SVM models. Zones preferentially populated by active and inactive molecules, respectively, clearly emerge in the class landscapes supported by the GTM model. Their analysis resulted in identification of privileged structural motifs of potential antimalarial compounds. Projection of marketed antimalarial drugs on this map allowed us to delineate several areas in the chemical space corresponding to different mechanisms of antimalarial activity. This helped us to make a suggestion about the mode of action of the molecules populating these zones.

Origami: Delineating Cosmic Structures with Phase-Space Folds

NASA Astrophysics Data System (ADS)

Neyrinck, Mark C.; Falck, Bridget L.; Szalay, Alex S.

2015-01-01

Structures like galaxies and filaments of galaxies in the Universe come about from the origami-like folding of an initially flat three-dimensional manifold in 6D phase space. The ORIGAMI method identifies these structures in a cosmological simulation, delineating the structures according to their outer folds. Structure identification is a crucial step in comparing cosmological simulations to observed maps of the Universe. The ORIGAMI definition is objective, dynamical and geometric: filament, wall and void particles are classified according to the number of orthogonal axes along which dark-matter streams have crossed. Here, we briefly review these ideas, and speculate on how ORIGAMI might be useful to find cosmic voids.

Genetic structure and viability selection in the golden eagle (Aquila chrysaetos), a vagile raptor with a Holarctic distribution

USGS Publications Warehouse

Doyle, Jacqueline M.; Katzner, Todd E.; Roemer, Gary; Cain, James W.; Millsap, Brian; McIntyre, Carol; Sonsthagen, Sarah A.; Fernandez, Nadia B.; Wheeler, Maria; Bulut, Zafer; Bloom, Peter; DeWoody, J. Andrew

2016-01-01

Molecular markers can reveal interesting aspects of organismal ecology and evolution, especially when surveyed in rare or elusive species. Herein, we provide a preliminary assessment of golden eagle (Aquila chrysaetos) population structure in North America using novel single nucleotide polymorphisms (SNPs). These SNPs included one molecular sexing marker, two mitochondrial markers, 85 putatively neutral markers that were derived from noncoding regions within large intergenic intervals, and 74 putatively nonneutral markers found in or very near protein-coding genes. We genotyped 523 eagle samples at these 162 SNPs and quantified genotyping error rates and variability at each marker. Our samples corresponded to 344 individual golden eagles as assessed by unique multilocus genotypes. Observed heterozygosity of known adults was significantly higher than of chicks, as was the number of heterozygous loci, indicating that mean zygosity measured across all 159 autosomal markers was an indicator of fitness as it is associated with eagle survival to adulthood. Finally, we used chick samples of known provenance to test for population differentiation across portions of North America and found pronounced structure among geographic sampling sites. These data indicate that cryptic genetic population structure is likely widespread in the golden eagle gene pool, and that extensive field sampling and genotyping will be required to more clearly delineate management units within North America and elsewhere.

A Parallel Population Genomic and Hydrodynamic Approach to Fishery Management of Highly-Dispersive Marine Invertebrates: The Case of the Fijian Black-Lip Pearl Oyster Pinctada margaritifera.

PubMed

Lal, Monal M; Southgate, Paul C; Jerry, Dean R; Bosserelle, Cyprien; Zenger, Kyall R

2016-01-01

Fishery management and conservation of marine species increasingly relies on genetic data to delineate biologically relevant stock boundaries. Unfortunately for high gene flow species which may display low, but statistically significant population structure, there is no clear consensus on the level of differentiation required to resolve distinct stocks. The use of fine-scale neutral and adaptive variation, considered together with environmental data can offer additional insights to this problem. Genome-wide genetic data (4,123 SNPs), together with an independent hydrodynamic particle dispersal model were used to inform farm and fishery management in the Fijian black-lip pearl oyster Pinctada margaritifera, where comprehensive fishery management is lacking, and the sustainability of exploitation uncertain. Weak fine-scale patterns of population structure were detected, indicative of broad-scale panmixia among wild oysters, while a hatchery-sourced farmed population exhibited a higher degree of genetic divergence (Fst = 0.0850-0.102). This hatchery-produced population had also experienced a bottleneck (NeLD = 5.1; 95% C.I. = [5.1-5.3]); compared to infinite NeLD estimates for all wild oysters. Simulation of larval transport pathways confirmed the existence of broad-scale mixture by surface ocean currents, correlating well with fine-scale patterns of population structuring. Fst outlier tests failed to detect large numbers of loci supportive of selection, with 2-5 directional outlier SNPs identified (average Fst = 0.116). The lack of biologically significant population genetic structure, absence of evidence for local adaptation and larval dispersal simulation, all indicate the existence of a single genetic stock of P. margaritifera in the Fiji Islands. This approach using independent genomic and oceanographic tools has allowed fundamental insights into stock structure in this species, with transferability to other highly-dispersive marine taxa for their conservation and management.

396. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

396. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; EAST BAY CROSSING; CANTILEVER STRUCTURE; DETAILS I; DRG. NO. 68 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

397. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

397. Delineator Unknown Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; EAST BAY CROSSING; CANTILEVER STRUCTURE; DETAILS II; DRG. NO. 69 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

Splitting or lumping? A conservation dilemma exemplified by the critically endangered dama gazelle (Nanger dama).

PubMed

Senn, Helen; Banfield, Lisa; Wacher, Tim; Newby, John; Rabeil, Thomas; Kaden, Jennifer; Kitchener, Andrew C; Abaigar, Teresa; Silva, Teresa Luísa; Maunder, Mike; Ogden, Rob

2014-01-01

Managers of threatened species often face the dilemma of whether to keep populations separate to conserve local adaptations and minimize the risk of outbreeding, or whether to manage populations jointly to reduce loss of genetic diversity and minimise inbreeding. In this study we examine genetic relatedness and diversity in three of the five last remaining wild populations of dama gazelle and a number of captive populations, using mtDNA control region and cytochrome b data. Despite the sampled populations belonging to the three putative subspecies, which are delineated according to phenotypes and geographical location, we find limited evidence for phylogeographical structure within the data and no genetic support for the putative subspecies. In the light of these data we discuss the relevance of inbreeding depression, outbreeding depression, adaptive variation, genetic drift, and phenotypic variation to the conservation of the dama gazelle and make some recommendations for its future conservation management. The genetic data suggest that the best conservation approach is to view the dama gazelle as a single species without subspecific divisions.

Splitting or Lumping? A Conservation Dilemma Exemplified by the Critically Endangered Dama Gazelle (Nanger dama)

PubMed Central

Senn, Helen; Banfield, Lisa; Wacher, Tim; Newby, John; Rabeil, Thomas; Kaden, Jennifer; Kitchener, Andrew C.; Abaigar, Teresa; Silva, Teresa Luísa; Maunder, Mike; Ogden, Rob

2014-01-01

Managers of threatened species often face the dilemma of whether to keep populations separate to conserve local adaptations and minimize the risk of outbreeding, or whether to manage populations jointly to reduce loss of genetic diversity and minimise inbreeding. In this study we examine genetic relatedness and diversity in three of the five last remaining wild populations of dama gazelle and a number of captive populations, using mtDNA control region and cytochrome b data. Despite the sampled populations belonging to the three putative subspecies, which are delineated according to phenotypes and geographical location, we find limited evidence for phylogeographical structure within the data and no genetic support for the putative subspecies. In the light of these data we discuss the relevance of inbreeding depression, outbreeding depression, adaptive variation, genetic drift, and phenotypic variation to the conservation of the dama gazelle and make some recommendations for its future conservation management. The genetic data suggest that the best conservation approach is to view the dama gazelle as a single species without subspecific divisions. PMID:24956104

381. J.H.E., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

381. J.H.E., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; WEST BAY CROSSING; SUSPENDED STRUCTURE; ERECTION; DRG. NO. 43 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

Individual tree crown delineation using localized contour tree method and airborne LiDAR data in coniferous forests

NASA Astrophysics Data System (ADS)

Wu, Bin; Yu, Bailang; Wu, Qiusheng; Huang, Yan; Chen, Zuoqi; Wu, Jianping

2016-10-01

Individual tree crown delineation is of great importance for forest inventory and management. The increasing availability of high-resolution airborne light detection and ranging (LiDAR) data makes it possible to delineate the crown structure of individual trees and deduce their geometric properties with high accuracy. In this study, we developed an automated segmentation method that is able to fully utilize high-resolution LiDAR data for detecting, extracting, and characterizing individual tree crowns with a multitude of geometric and topological properties. The proposed approach captures topological structure of forest and quantifies topological relationships of tree crowns by using a graph theory-based localized contour tree method, and finally segments individual tree crowns by analogy of recognizing hills from a topographic map. This approach consists of five key technical components: (1) derivation of canopy height model from airborne LiDAR data; (2) generation of contours based on the canopy height model; (3) extraction of hierarchical structures of tree crowns using the localized contour tree method; (4) delineation of individual tree crowns by segmenting hierarchical crown structure; and (5) calculation of geometric and topological properties of individual trees. We applied our new method to the Medicine Bow National Forest in the southwest of Laramie, Wyoming and the HJ Andrews Experimental Forest in the central portion of the Cascade Range of Oregon, U.S. The results reveal that the overall accuracy of individual tree crown delineation for the two study areas achieved 94.21% and 75.07%, respectively. Our method holds great potential for segmenting individual tree crowns under various forest conditions. Furthermore, the geometric and topological attributes derived from our method provide comprehensive and essential information for forest management.

COMPLETED FERTILITY DURING THE TWENTIETH CENTURY: AN EXAMPLE FROM SIX SETTLEMENTS IN NORTHERN GREECE.

PubMed

Zafeiris, Konstantinos N; Kaklamani, Stamatina

2018-02-06

This study aimed to delineate temporal trends and differentials of completed fertility and their relationship with some characteristics of the marriage system in specific anthropological populations of northern Greece. The analysis was based on the life history of quinquennial and decennial birth cohorts of married women born in the 20th century who reproduced solely within the settlements studied. The variables studied were: children ever born, mean age of mother at first marriage, mean age of mother at first child (live birth), mean age of mother at last child and reproductive span. The results indicated that there were significant differences in the demographic characteristics of marriage and that there was an ongoing fertility transition in the 20th century in the populations studied. The mechanism of fertility decline was connected with the gradual reduction of the mean age of the mother at last child, the parallel decrease in the mean age at childbearing and a shortening of the reproductive span. Fertility levels at all times maintained a dynamic character imposed by local cultural, economic and social structures, which, in turn, were part of broader national and international structures, in all the populations studied. A strong trend of convergence of fertility levels was observed among the populations studied.

Lack of spatial genetic structure among nesting and wintering King Eiders

USGS Publications Warehouse

Pearce, J.M.; Talbot, S.L.; Pierson, Barbara J.; Petersen, M.R.; Scribner, K.T.; Dickson, D.L.; Mosbech, A.

2004-01-01

The King Eider (Somateria spectabilis) has been delineated into two broadly distributed breeding populations in North America (the western and eastern Arctic) on the basis of banding data and their use of widely separated Pacific and Atlantic wintering areas. Little is known about the level of gene flow between these two populations. Also unknown is whether behavioral patterns common among migratory waterfowl, such as site fidelity to wintering areas and pair formation at these sites, have existed for sufficient time to create a population structure defined by philopatry to wintering rather than to nesting locations. We used six nuclear microsatellite DNA loci and cytochrome b mitochondrial DNA sequence data to estimate the extent of spatial genetic differentiation among nesting and wintering areas of King Eiders across North America and adjacent regions. Estimates of interpopulation variance in microsatellite allele and mtDNA haplotype frequency were both low and nonsignificant based on samples from three wintering and four nesting areas. Results from nested clade analysis, mismatch distributions, and coalescent-based analyses suggest historical population growth and gene flow that collectively may have homogenized gene frequencies. The presence of several unique mtDNA haplotypes among birds wintering near Greenland suggests that gene flow may now be more limited between the western and eastern Arctic, which is consistent with banding data.

Latitude delineates patterns of biogeography in terrestrial Streptomyces.

PubMed

Choudoir, Mallory J; Doroghazi, James R; Buckley, Daniel H

2016-12-01

The biogeography of Streptomyces was examined at regional spatial scales to identify factors that govern patterns of microbial diversity. Streptomyces are spore forming filamentous bacteria which are widespread in soil. Streptomyces strains were isolated from perennial grass habitats sampled across a spatial scale of more than 6000 km. Previous analysis of this geographically explicit culture collection provided evidence for a latitudinal diversity gradient in Streptomyces species. Here the hypothesis that this latitudinal diversity gradient is a result of evolutionary dynamics associated with historical demographic processes was evaluated. Historical demographic phenomena have genetic consequences that can be evaluated through analysis of population genetics. Population genetic approaches were applied to analyze population structure in six of the most numerically abundant and geographically widespread Streptomyces phylogroups from our culture collection. Streptomyces population structure varied at regional spatial scales, and allelic diversity correlated with geographic distance. In addition, allelic diversity and gene flow are partitioned by latitude. Finally, it was found that nucleotide diversity within phylogroups was negatively correlated with latitude. These results indicate that phylogroup diversification is constrained by dispersal limitation at regional spatial scales, and they are consistent with the hypothesis that historical demographic processes have influenced the contemporary biogeography of Streptomyces. © 2016 Society for Applied Microbiology and John Wiley & Sons Ltd.

Evaluation of genetic population structure of smallmouth bass in the Susquehanna River basin, Pennsylvania

USGS Publications Warehouse

Schall, Megan K.; Bartron, Meredith L.; Wertz, Timothy; Niles, Jonathan M.; Shaw, Cassidy H.; Wagner, Tyler

2017-01-01

The Smallmouth Bass Micropterus dolomieu was introduced into the Susquehanna River basin, Pennsylvania, nearly 150 years ago. Since introduction, it has become an economically and ecologically important species that supports popular recreational fisheries. It is also one of the most abundant top predators in the system. Currently, there is no information on the level of genetic diversity or genetic structuring that may have occurred since introduction. An understanding of genetic diversity is important for the delineation of management units and investigation of gene flow at various management scales. The goals of this research were to investigate population genetic structure of Smallmouth Bass at sites within the Susquehanna River basin and to assess genetic differentiation relative to Smallmouth Bass at an out-of-basin site (Allegheny River, Pennsylvania) located within the species’ native range. During spring 2015, fin clips (n = 1,034) were collected from adults at 11 river sites and 13 tributary sites in the Susquehanna River basin and at one site on the Allegheny River. Fin clips were genotyped at 12 polymorphic microsatellite loci. Based on our results, adults sampled throughout the Susquehanna River basin did not represent separate genetic populations. There were only subtle differences in genetic diversity among sites (mean pairwise genetic differentiation index FST = 0.012), and there was an overall lack of population differentiation (K = 3 admixed populations). The greatest genetic differentiation was observed between fish collected from the out-of-basin site and those from the Susquehanna River basin sites. Knowledge that separate genetic populations of Smallmouth Bass do not exist in the Susquehanna River basin is valuable information for fisheries management in addition to providing baseline genetic data on an introduced sport fish population.

Delineating the Structure of Normal and Abnormal Personality: An Integrative Hierarchical Approach

PubMed Central

Markon, Kristian E.; Krueger, Robert F.; Watson, David

2008-01-01

Increasing evidence indicates that normal and abnormal personality can be treated within a single structural framework. However, identification of a single integrated structure of normal and abnormal personality has remained elusive. Here, a constructive replication approach was used to delineate an integrative hierarchical account of the structure of normal and abnormal personality. This hierarchical structure, which integrates many Big Trait models proposed in the literature, replicated across a meta-analysis as well as an empirical study, and across samples of participants as well as measures. The proposed structure resembles previously suggested accounts of personality hierarchy and provides insight into the nature of personality hierarchy more generally. Potential directions for future research on personality and psychopathology are discussed. PMID:15631580

Delineating the structure of normal and abnormal personality: an integrative hierarchical approach.

PubMed

Markon, Kristian E; Krueger, Robert F; Watson, David

2005-01-01

Increasing evidence indicates that normal and abnormal personality can be treated within a single structural framework. However, identification of a single integrated structure of normal and abnormal personality has remained elusive. Here, a constructive replication approach was used to delineate an integrative hierarchical account of the structure of normal and abnormal personality. This hierarchical structure, which integrates many Big Trait models proposed in the literature, replicated across a meta-analysis as well as an empirical study, and across samples of participants as well as measures. The proposed structure resembles previously suggested accounts of personality hierarchy and provides insight into the nature of personality hierarchy more generally. Potential directions for future research on personality and psychopathology are discussed.

389. J.H.E., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

389. J.H.E., Delineator Date Unknown STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; WEST BAY CROSSING; SUSPENDED STRUCTURE; TYPICAL DETAILS; DRG. NO. 42 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

SU-E-J-159: Intra-Patient Deformable Image Registration Uncertainties Quantified Using the Distance Discordance Metric

DOE Office of Scientific and Technical Information (OSTI.GOV)

Saleh, Z; Thor, M; Apte, A

2014-06-01

Purpose: The quantitative evaluation of deformable image registration (DIR) is currently challenging due to lack of a ground truth. In this study we test a new method proposed for quantifying multiple-image based DIRrelated uncertainties, for DIR of pelvic images. Methods: 19 patients were analyzed, each with 6 CT scans, who previously had radiotherapy for prostate cancer. Manually delineated structures for rectum and bladder, which served as ground truth structures, were delineated on the planning CT and each subsequent scan. For each patient, voxel-by-voxel DIR-related uncertainties were evaluated, following B-spline based DIR, by applying a previously developed metric, the distance discordancemore » metric (DDM; Saleh et al., PMB (2014) 59:733). The DDM map was superimposed on the first acquired CT scan and DDM statistics were assessed, also relative to two metrics estimating the agreement between the propagated and the manually delineated structures. Results: The highest DDM values which correspond to greatest spatial uncertainties were observed near the body surface and in the bowel due to the presence of gas. The mean rectal and bladder DDM values ranged from 1.1–11.1 mm and 1.5–12.7 mm, respectively. There was a strong correlation in the DDMs between the rectum and bladder (Pearson R = 0.68 for the max DDM). For both structures, DDM was correlated with the ratio between the DIR-propagated and manually delineated volumes (R = 0.74 for the max rectal DDM). The maximum rectal DDM was negatively correlated with the Dice Similarity Coefficient between the propagated and the manually delineated volumes (R= −0.52). Conclusion: The multipleimage based DDM map quantified considerable DIR variability across different structures and among patients. Besides using the DDM for quantifying DIR-related uncertainties it could potentially be used to adjust for uncertainties in DIR-based accumulated dose distributions.« less

Semi-automated stand delineation in Mediterranean Pinus sylvestris plantations through segmentation of LiDAR data: The influence of pulse density

NASA Astrophysics Data System (ADS)

Varo-Martínez, Mª Ángeles; Navarro-Cerrillo, Rafael M.; Hernández-Clemente, Rocío; Duque-Lazo, Joaquín

2017-04-01

Traditionally, forest-stand delineation has been assessed based on orthophotography. The application of LiDAR has improved forest management by providing high-spatial-resolution data on the vertical structure of the forest. The aim of this study was to develop and test a semi-automated algorithm for stands delineation in a plantation of Pinus sylvestris L. using LiDAR data. Three specific objectives were evaluated, i) to assess two complementary LiDAR metrics, Assmann dominant height and basal area, for the characterization of the structure of P. sylvestris Mediterranean forests based on object-oriented segmentation, ii) to evaluate the influence of the LiDAR pulse density on forest-stand delineation accuracy, and iii) to investigate the algorithmś effectiveness in the delineation of P. sylvestris stands for map prediction of Assmann dominant height and basal area. Our results show that it is possible to generate accurate P. sylvestris forest-stand segmentations using multiresolution or mean shift segmentation methods, even with low-pulse-density LiDAR - which is an important economic advantage for forest management. However, eCognition multiresolution methods provided better results than the OTB (Orfeo Tool Box) for stand delineation based on dominant height and basal area estimations. Furthermore, the influence of pulse density on the results was not statistically significant in the basal area calculations. However, there was a significant effect of pulse density on Assmann dominant height [F2,9595 = 5.69, p = 0.003].for low pulse density. We propose that the approach shown here should be considered for stand delineation in other large Pinus plantations in Mediterranean regions with similar characteristics.

Reliably Discriminating Stock Structure with Genetic Markers:Mixture Models with Robust and Fast Computation.

PubMed

Foster, Scott D; Feutry, Pierre; Grewe, Peter M; Berry, Oliver; Hui, Francis K C; Davies, Campbell R

2018-06-26

Delineating naturally occurring and self-sustaining sub-populations (stocks) of a species is an important task, especially for species harvested from the wild. Despite its central importance to natural resource management, analytical methods used to delineate stocks are often, and increasingly, borrowed from superficially similar analytical tasks in human genetics even though models specifically for stock identification have been previously developed. Unfortunately, the analytical tasks in resource management and human genetics are not identical { questions about humans are typically aimed at inferring ancestry (often referred to as 'admixture') rather than breeding stocks. In this article, we argue, and show through simulation experiments and an analysis of yellowfin tuna data, that ancestral analysis methods are not always appropriate for stock delineation. In this work, we advocate a variant of a previouslyintroduced and simpler model that identifies stocks directly. We also highlight that the computational aspects of the analysis, irrespective of the model, are difficult. We introduce some alternative computational methods and quantitatively compare these methods to each other and to established methods. We also present a method for quantifying uncertainty in model parameters and in assignment probabilities. In doing so, we demonstrate that point estimates can be misleading. One of the computational strategies presented here, based on an expectation-maximisation algorithm with judiciously chosen starting values, is robust and has a modest computational cost. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

Individual Tree Crown Delineation Using Multi-Wavelength Titan LIDAR Data

NASA Astrophysics Data System (ADS)

Naveed, F.; Hu, B.

2017-10-01

The inability to detect the Emerald Ash Borer (EAB) at an early stage has led to the enumerable loss of different species of ash trees. Due to the increasing risk being posed by the EAB, a robust and accurate method is needed for identifying Individual Tree Crowns (ITCs) that are at a risk of being infected or are already diseased. This paper attempts to outline an ITC delineation method that employs airborne multi-spectral Light Detection and Ranging (LiDAR) to accurately delineate tree crowns. The raw LiDAR data were initially pre-processed to generate the Digital Surface Models (DSM) and Digital Elevation Models (DEM) using an iterative progressive TIN (Triangulated Irregular Network) densification method. The DSM and DEM were consequently used for Canopy Height Model (CHM) generation, from which the structural information pertaining to the size and shape of the tree crowns was obtained. The structural information along with the spectral information was used to segment ITCs using a region growing algorithm. The availability of the multi-spectral LiDAR data allows for delineation of crowns that have otherwise homogenous structural characteristics and hence cannot be isolated from the CHM alone. This study exploits the spectral data to derive initial approximations of individual tree tops and consequently grow those regions based on the spectral constraints of the individual trees.

388. D.E.M., Delineator December 1932 STATE OF CALIFORNIA; DEPARTMENT OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

388. D.E.M., Delineator December 1932 STATE OF CALIFORNIA; DEPARTMENT OF PUBLIC WORKS; SAN FRANCISCO - OAKLAND BAY BRIDGE; SUPERSTRUCTURE - WEST BAY CROSSING; SUSPENDED STRUCTURE; SIDE SPAN TRUSSES AT ANCHORAGES; CONTRACT NO. 6; DRAWING NO. 40 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

Laparoscopy-guided intracorporeal ultrasound accurately delineates hepatobiliary anatomy.

PubMed

Yamamoto, M; Stiegmann, G V; Durham, J; Berguer, R; Oba, Y; Fujiyama, Y; McIntyre, R C

1993-01-01

The purpose of this study was to develop a technique and assess the ability of a laparoscopic ultrasound probe to delineate biliary antomy and to determine the presence or absence of duct stones. Five pigs had ultrasonography of biliary structures and liver at laparoscopy followed by cholangiograms and anatomical dissection. Five patients had ultrasonography of the biliary tract at laparoscopic cholecystectomy. All animals had adequate visualization of important hepatobiliary structure, and an optimal method of accessing these structures at laparoscopy was established. Patients had ultrasonography which used methods developed in the animal trial. All had adequate visualization of the entire common bile duct confirmed by cholangiography. Limitations in demonstrating the relationship of the cystic duct to the common duct were technical and can be corrected. Laparoscopic ultrasonography has significant potential for delineation of biliary anatomy and determination of presence or absence of duct calculi. Clinical implementation could minimize the risk of iatrogenic duct injury and the need for operative cholangiography.

Assessing the resolution of haplotype distributions to delineate fall armyworm (Lepidoptera:Noctuidae) migratory behaviors

USDA-ARS?s Scientific Manuscript database

Regions of southern Florida and southern Texas (extending into Mexico) provide the overwintering source populations for virtually all fall armyworm infestations affecting the continental U.S. Understanding how these migratory populations annually disperse is important to predict and control infestat...

What can otolith shape analysis tell us about population structure of the European sardine, Sardina pilchardus, from Atlantic and Mediterranean waters?

NASA Astrophysics Data System (ADS)

Jemaa, Sharif; Bacha, Mahmoud; Khalaf, Gaby; Dessailly, David; Rabhi, Khalef; Amara, Rachid

2015-02-01

The European sardine, Sardina pilchardus, exhibits a complex population structure, which has produced conflicting results in previous genetic studies. Despite its importance in the fisheries industry, stock delineation for management and conservation purposes is still a matter of debate throughout the distribution range of the species. This study examines whether otolith shapes are more efficient than genetic markers to detect population structure in pelagic species with large population sizes. Sardines were analyzed from 15 sampling localities in the Northeast Atlantic and Mediterranean Sea covering almost the whole distribution range of the species. A combination of otolith shape indices and elliptic Fourier descriptors was investigated by multivariate statistical procedures. Within the studied area, three distinct groups were identified with an overall correct classification of 77%. Group A: northern Mediterranean Sea and Gulf of Gabès; group B: Atlantic Morocco-south Alboran-Algero-provençal coasts; and group C: European Atlantic coast. The Almeria-Oran front and the Gibraltar strait are not an efficient barrier for sardine population separation as there seems to be exchanges between populations of the south-western Mediterranean Sea and those of the Moroccan Atlantic Ocean coast or Gulf of Cadiz. The results are discussed in relation to environmental conditions, oceanographic features, and physical barriers to dispersal in the study area, and compared with those obtained by previous genetic, morphometric, and meristic data. For pelagic species with high gene flow, present results highlighted the need to take into account the identification of phenotypic stocks to ensure sustainable fishery benefits and efficient conservation as they may have unique demographic properties and responses to exploitation.

Extension: The Backbone Organization in Statewide Population Health Management

ERIC Educational Resources Information Center

Parisi, Michelle A.; Northcutt, Julie K.; McKendry, Jean E.; Sherrill, Windsor Westbrook; Dye, Cheryl J.; Snow, Jennifer Z.

2018-01-01

Clemson University Cooperative Extension Service has developed and is implementing a statewide population health management model aimed at leveraging key partnerships in South Carolina. The model delineates roles of key partners needed to operationalize statewide initiatives and drive the delivery of health extension in the state. Clemson…

Strong trans-Pacific break and local conservation units in the Galapagos shark (Carcharhinus galapagensis) revealed by genome-wide cytonuclear markers.

PubMed

Pazmiño, Diana A; Maes, Gregory E; Green, Madeline E; Simpfendorfer, Colin A; Hoyos-Padilla, E Mauricio; Duffy, Clinton J A; Meyer, Carl G; Kerwath, Sven E; Salinas-de-León, Pelayo; van Herwerden, Lynne

2018-05-01

The application of genome-wide cytonuclear molecular data to identify management and adaptive units at various spatio-temporal levels is particularly important for overharvested large predatory organisms, often characterized by smaller, localized populations. Despite being "near threatened", current understanding of habitat use and population structure of Carcharhinus galapagensis is limited to specific areas within its distribution. We evaluated population structure and connectivity across the Pacific Ocean using genome-wide single-nucleotide polymorphisms (~7200 SNPs) and mitochondrial control region sequences (945 bp) for 229 individuals. Neutral SNPs defined at least two genetically discrete geographic groups: an East Tropical Pacific (Mexico, east and west Galapagos Islands), and another central-west Pacific (Lord Howe Island, Middleton Reef, Norfolk Island, Elizabeth Reef, Kermadec, Hawaii and Southern Africa). More fine-grade population structure was suggested using outlier SNPs: west Pacific, Hawaii, Mexico, and Galapagos. Consistently, mtDNA pairwise Φ ST defined three regional stocks: east, central and west Pacific. Compared to neutral SNPs (F ST  = 0.023-0.035), mtDNA exhibited more divergence (Φ ST  = 0.258-0.539) and high overall genetic diversity (h = 0.794 ± 0.014; π = 0.004 ± 0.000), consistent with the longstanding eastern Pacific barrier between the east and central-west Pacific. Hawaiian and Southern African populations group within the west Pacific cluster. Effective population sizes were moderate/high for east/west populations (738 and 3421, respectively). Insights into the biology, connectivity, genetic diversity, and population demographics informs for improved conservation of this species, by delineating three to four conservation units across their Pacific distribution. Implementing such conservation management may be challenging, but is necessary to achieve long-term population resilience at basin and regional scales.

Differences in delineation guidelines for head and neck cancer result in inconsistent reported dose and corresponding NTCP.

PubMed

Brouwer, Charlotte L; Steenbakkers, Roel J H M; Gort, Elske; Kamphuis, Marije E; van der Laan, Hans Paul; Van't Veld, Aart A; Sijtsema, Nanna M; Langendijk, Johannes A

2014-04-01

To test the hypothesis that delineation of swallowing organs at risk (SWOARs) based on different guidelines results in differences in dose-volume parameters and subsequent normal tissue complication probability (NTCP) values for dysphagia-related endpoints. Nine different SWOARs were delineated according to five different delineation guidelines in 29 patients. Reference delineation was performed according to the guidelines and NTCP-models of Christianen et al. Concordance Index (CI), dosimetric consequences, as well as differences in the subsequent NTCPs were calculated. The median CI of the different delineation guidelines with the reference guidelines was 0.54 for the pharyngeal constrictor muscles, 0.56 for the laryngeal structures and 0.07 for the cricopharyngeal muscle and esophageal inlet muscle. The average difference in mean dose to the SWOARs between the guidelines with the largest difference (maxΔD) was 3.5±3.2Gy. A mean ΔNTCP of 2.3±2.7% was found. For two patients, ΔNTCP exceeded 10%. The majority of the patients showed little differences in NTCPs between the different delineation guidelines. However, large NTCP differences >10% were found in 7% of the patients. For correct use of NTCP models in individual patients, uniform delineation guidelines are of great importance. Copyright © 2014 The Authors. Published by Elsevier Ireland Ltd.. All rights reserved.

Automatic segmentation software in locally advanced rectal cancer: READY (REsearch program in Auto Delineation sYstem)-RECTAL 02: prospective study.

PubMed

Gambacorta, Maria A; Boldrini, Luca; Valentini, Chiara; Dinapoli, Nicola; Mattiucci, Gian C; Chiloiro, Giuditta; Pasini, Danilo; Manfrida, Stefania; Caria, Nicola; Minsky, Bruce D; Valentini, Vincenzo

2016-07-05

To validate autocontouring software (AS) in a clinical practice including a two steps delineation quality assurance (QA) procedure.The existing delineation agreement among experts for rectal cancer and the overlap and time criteria that have to be verified to allow the use of AS were defined.Median Dice Similarity Coefficient (MDSC), Mean slicewise Hausdorff Distances (MSHD) and Total-Time saving (TT) were analyzed.Two expert Radiation Oncologists reviewed CT-scans of 44 patients and agreed the reference-CTV: the first 14 consecutive cases were used to populate the software Atlas and 30 were used as Test.Each expert performed a manual (group A) and an automatic delineation (group B) of 15 Test patients.The delineations were compared with the reference contours.The overlap between the manual and automatic delineations with MDSC and MSHD and the TT were analyzed.Three acceptance criteria were set: MDSC ≥ 0.75, MSHD ≤1mm and TT sparing ≥ 50%.At least 2 criteria had to be met, one of which had to be TT saving, to validate the system.The MDSC was 0.75, MSHD 2.00 mm and the TT saving 55.5% between group A and group B. MDSC among experts was 0.84.Autosegmentation systems in rectal cancer partially met acceptability criteria with the present version.

SU-F-T-405: Development of a Rapid Cardiac Contouring Tool Using Landmark-Driven Modeling

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pelletier, C; Jung, J; Mosher, E

2016-06-15

Purpose: This study aims to develop a tool to rapidly delineate cardiac substructures for use in dosimetry for large-scale clinical trial or epidemiological investigations. The goal is to produce a system that can semi-automatically delineate nine cardiac structures to a reasonable accuracy within a couple of minutes. Methods: The cardiac contouring tool employs a Most Similar Atlas method, where a selection criterion is used to pre-select the most similar model to the patient from a library of pre-defined atlases. Sixty contrast-enhanced cardiac computed tomography angiography (CTA) scans (30 male and 30 female) were manually contoured to serve as the atlasmore » library. For each CTA 12 structures were delineated. Kabsch algorithm was used to compute the optimum rotation and translation matrices between the patient and atlas. Minimum root mean squared distance between the patient and atlas after transformation was used to select the most-similar atlas. An initial study using 10 CTA sets was performed to assess system feasibility. Leave-one patient out method was performed, and fit criteria were calculated to evaluate the fit accuracy compared to manual contours. Results: For the pilot study, mean dice indices of .895 were achieved for the whole heart, .867 for the ventricles, and .802 for the atria. In addition, mean distance was measured via the chord length distribution (CLD) between ground truth and the atlas structures for the four coronary arteries. The mean CLD for all coronary arteries was below 14mm, with the left circumflex artery showing the best agreement (7.08mm). Conclusion: The cardiac contouring tool is able to delineate cardiac structures with reasonable accuracy in less than 90 seconds. Pilot data indicates that the system is able to delineate the whole heart and ventricles within a reasonable accuracy using even a limited library. We are extending the atlas sets to 60 adult males and females in total.« less

Integrating aeromagnetic and Landsat™ 8 data into subsurface structural mapping of Precambrian basement complex

NASA Astrophysics Data System (ADS)

Kayode, John Stephen; Nawawi, M. N. M.; Abdullah, Khiruddin B.; Khalil, Amin E.

2017-01-01

The integration of Aeromagnetic data and remotely sensed imagery with the intents of mapping the subsurface geological structures in part of the South-western basement complex of Nigeria was developed using the PCI Geomatica Software. 2013. The data obtained from the Nigerian Geological Survey Agency; was corrected using Regional Residual Separation of the Total Magnetic field anomalies enhanced, and International Geomagnetic Reference Field removed. The principal objective of this study is, therefore, to introduce a rapid and efficient method of subsurface structural depth estimate and structural index evaluation through the incorporation of the Euler Deconvolution technique into PCI Geomatica 2013 to prospect for subsurface geological structures. The shape and depth of burial helped to define these structures from the regional aeromagnetic map. The method enabled various structural indices to be automatically delineated for an index of between 0.5 SI and 3.0 SI at a maximum depth of 1.1 km that clearly showed the best depths estimate for all the structural indices. The results delineate two major magnetic belts in the area; the first belt shows an elongated ridge-like structure trending mostly along the NorthNortheast-SouthSouthwest and the other anomalies trends primarily in the Northeast, Northwest, Northeast-Southwest parts of the study area that could be attributed to basement complex granitic intrusions from the tectonic history of the area. The majority of the second structures showed various linear structures different from the first structure. Basically, a significant offset was delineated at the core segment of the study area, suggesting a major subsurface geological feature that controls mineralisation in this area.

Claustrum of the short-tailed fruit bat, Carollia perspicillata: Alignment of cellular orientation and functional connectivity.

PubMed

Orman, Rena; Kollmar, Richard; Stewart, Mark

2017-04-15

The claustrum is a gray-matter structure that underlies neocortex and reciprocates connections with cortical and subcortical targets. In lower mammals, the claustrum is directly adjacent to neocortex, making the definition of claustral boundaries challenging. Latexin, an endogenous inhibitor of metallocarboxypeptidases, localizes to claustral cells, enabling a clear delineation of claustrum. Given its proportionately large claustrum, we hypothesized that the short-tailed fruit bat, Carollia perspicillata, can be a useful model for claustral structure-function relations. We used latexin immunohistochemistry to identify claustral boundaries and intrinsic structure and multielectrode recordings from brain slices to explore intrinsic excitatory connectivity of the claustrum. Carollia's claustrum contains cells whose intrinsic connectivity and alignment permit the generation of spontaneous, synchronous population events and mirror their pattern of spread in disinhibited brain slices over millimeters. Carollia shows cellular alignment and spontaneous population-activity spread along both horizontal and dorsoventral axes. Carollia claustrum possesses intrinsic excitatory connectivity sufficient to: 1) generate single, spontaneous, synchronized burst discharges, 2) support activity spread along axes where claustral cells are aligned, and 3), because of multiple axes for cell alignment, support activity spread along both rostrocaudal and dorsoventral axes. The smaller event sizes in bat claustrum compared with rat claustrum are consistent with events occurring in population subsets rather than the full claustral cell population. The overall size of claustrum, its pronounced vascularity, and its more complex intrinsic connectivity than rat suggest that the bat is an animal model for claustral structure and function that will permit unique access to claustrum's processing capabilities. J. Comp. Neurol. 525:1459-1474, 2017. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

Validation Theory and Research for a Population-Level Measure of Children's Development, Wellbeing, and School Readiness

ERIC Educational Resources Information Center

Guhn, Martin; Zumbo, Bruno D.; Janus, Magdalena; Hertzman, Clyde

2011-01-01

This paper delineates general validity and research questions that are underlying an ongoing program of research pertaining to the Early Development Instrument (EDI, Janus and Offord 2007), a population-level measure, on which teachers rate kindergarten children's developmental outcomes in the social, emotional, physical, cognitive, and…

Steelhead of the south-central/southern California coast: Population characterization for recovery planning

USGS Publications Warehouse

Boughton, David A.; Adams, P.B.; Anderson, E.; Fusaro, Craig; Keller, E.; Kelley, Elsie; Lentsch, Leo; Nielsen, J. L.; Perry, Katie; Regan, Helen; Swift, C.; Watson, Fred

2006-01-01

This report by the National Marine Fisheries Service applies a formal evaluation framework to the problem of delineating Oncorhynchus mykiss populations in the South-Central/Southern California Coast recovery domain, in support of recovery planning under the Endangered Species Act.

POPULATION STATUS AND DISTRIBUTION OF A DECIMATED AMPHIBIAN, THE RELICT LEOPARD FROG (RANA ONCA)

EPA Science Inventory

The relict leopard frog (Rana onca) was once thought to be extinct, but has recently been shown to comprise a valid taxon with extant populations. We delineate the minimum historical range of the species, and report results of surveys at 12 historical and 54 other localities to d...

Smoking Cessation: Next Steps for Special Populations Research and Innovative Treatments

ERIC Educational Resources Information Center

Borrelli, Belinda

2010-01-01

Objective: The current introductory article provides the rationale for the special section on understudied smokers and innovative treatments. This article proposes a definition of "special populations" of smokers, outlines a priori criteria by which to judge whether an intervention should be adapted for these smokers, and delineates a process by…

Techniques of contributing-area delineation for analysis of nonpoint-source contamination of Long Island, New York

USGS Publications Warehouse

Misut, P.

1995-01-01

Ninety shallow monitoring wells on Long Island, N.Y., were used to test the hypothesis that the correlation between the detection of volatile organic compounds (VOC's) at a well and explanatory variables representing land use, population density, and hydrogeologic conditions around the well is affected by the size and shape of the area defined as the contributing area. Explanatory variables are quantified through overlay of various specified contributing areas on 1:24 000-scale landuse and population-density geographic information system (GIS) coverages. Four methods of contributing-area delineation were used: (a) centering a circle of selected radius on the well site, (b) orienting a triangular area along the direction of horizontal ground-water flow to the well, (c) generating a shaped based on direction and magnitude of horizontal flow to the well, and (d) generating a shape based on three-dimensional particle pathlines backtracked from the well screen to the water table. The strongest correlations with VOC detections were obtained from circles of 400- to 1 000-meter radius. Improvement in correlation through delineations based on ground-water flow would require geographic overlay on more highly detailed GIS coverages than those used in the study.

Population genetic structure of clinical and environmental isolates of Blastomyces dermatitidis, Based on 27 Polymorphic Microsatellite Markers

USGS Publications Warehouse

Meece, J.K.; Anderson, J.L.; Fisher, M.C.; Henk, D.A.; Sloss, Brian L.; Reed, K.D.

2011-01-01

Blastomyces dermatitidis, a thermally dimorphic fungus, is the etiologic agent of North American blastomycosis. Clinical presentation is varied, ranging from silent infections to fulminant respiratory disease and dissemination to skin and other sites. Exploration of the population genetic structure of B. dermatitidis would improve our knowledge regarding variation in virulence phenotypes, geographic distribution, and difference in host specificity. The objective of this study was to develop and test a panel of microsatellite markers to delineate the population genetic structure within a group of clinical and environmental isolates of B. dermatitidis. We developed 27 microsatellite markers and genotyped B. dermatitidis isolates from various hosts and environmental sources (n = 112). Assembly of a neighbor-joining tree of allele-sharing distance revealed two genetically distinct groups, separated by a deep node. Bayesian admixture analysis showed that two populations were statistically supported. Principal coordinate analysis also reinforced support for two genetic groups, with the primary axis explaining 61.41% of the genetic variability. Group 1 isolates average 1.8 alleles/locus, whereas group 2 isolates are highly polymorphic, averaging 8.2 alleles/locus. In this data set, alleles at three loci are unshared between the two groups and appear diagnostic. The mating type of individual isolates was determined by PCR. Both mating type-specific genes, the HMG and ??-box domains, were represented in each of the genetic groups, with slightly more isolates having the HMG allele. One interpretation of this study is that the species currently designated B. dermatitidis includes a cryptic subspecies or perhaps a separate species. ?? 2011, American Society for Microbiology.

Population genetic structure of clinical and environmental isolates of Blastomyces dermatitidis based on 27 polymorphic microsatellite markers

USGS Publications Warehouse

Meece, Jennifer K.; Anderson, Jennifer L.; Fisher, Matthew C.; Henk, Daniel A.; Sloss, Brian L.; Reed, Kurt D.

2011-01-01

Blastomyces dermatitidis, a thermally dimorphic fungus, is the etiologic agent of North American blastomycosis. Clinical presentation is varied, ranging from silent infections to fulminant respiratory disease and dissemination to skin and other sites. Exploration of the population genetic structure of B. dermatitidis would improve our knowledge regarding variation in virulence phenotypes, geographic distribution, and difference in host specificity. The objective of this study was to develop and test a panel of microsatellite markers to delineate the population genetic structure within a group of clinical and environmental isolates of B. dermatitidis. We developed 27 microsatellite markers and genotyped B. dermatitidis isolates from various hosts and environmental sources (n=112). Assembly of a neighbor-joining tree of allele-sharing distance revealed two genetically distinct groups, separated by a deep node. Bayesian admixture analysis showed that two populations were statistically supported. Principal coordinate analysis also reinforced support for two genetic groups, with the primary axis explaining 61.41% of the genetic variability. Group 1 isolates average 1.8 alleles/locus, whereas group 2 isolates are highly polymorphic, averaging 8.2 alleles/locus. In this data set, alleles at three loci are unshared between the two groups and appear diagnostic. The mating type of individual isolates was determined by PCR. Both mating type-specific genes, the HMG and α-box domains, were represented in each of the genetic groups, with slightly more isolates having the HMG allele. One interpretation of this study is that the species currently designated B. dermatitidis includes a cryptic subspecies or perhaps a separate species.

[The research protocol III. Study population].

PubMed

Arias-Gómez, Jesús; Villasís-Keever, Miguel Ángel; Miranda-Novales, María Guadalupe

2016-01-01

The study population is defined as a set of cases, determined, limited, and accessible, that will constitute the subjects for the selection of the sample, and must fulfill several characteristics and distinct criteria. The objectives of this manuscript are focused on specifying each one of the elements required to make the selection of the participants of a research project, during the elaboration of the protocol, including the concepts of study population, sample, selection criteria and sampling methods. After delineating the study population, the researcher must specify the criteria that each participant has to comply. The criteria that include the specific characteristics are denominated selection or eligibility criteria. These criteria are inclusion, exclusion and elimination, and will delineate the eligible population. The sampling methods are divided in two large groups: 1) probabilistic or random sampling and 2) non-probabilistic sampling. The difference lies in the employment of statistical methods to select the subjects. In every research, it is necessary to establish at the beginning the specific number of participants to be included to achieve the objectives of the study. This number is the sample size, and can be calculated or estimated with mathematical formulas and statistic software.

Remote sensing and GIS approach for water-well site selection, southwest Iran

USGS Publications Warehouse

Rangzan, K.; Charchi, A.; Abshirini, E.; Dinger, J.

2008-01-01

The Pabdeh-Lali Anticline of northern Khuzestan province is located in southwestern Iran and occupies 790 km2. This structure is situated in the Zagros folded belt. As a result of well-developed karst systems in the anticlinal axis, the water supply potential is high and is drained by many peripheral springs. However, there is a scarcity of water for agriculture and population centers on the anticlinal flanks, which imposes a severe problem in terms of area development. This study combines remotely sensed (RS) data and a geographical information system (GIS) into a RSGIS technique to delineate new areas for groundwater development and specific sites for drilling productive water wells. Toward these goals, RS data were used to develop GIS layers for lithology, structural geology, topographic slope, elevation, and drainage density. Field measurements were made to create spring-location and groundwater-quality GIS layers. Subsequently, expert choice and relational methods were used in a GIS environment to conjunctively analyze all layers to delineate preferable regions and 43 individual sites in which to drill water wells. Results indicate that the most preferred areas are, in preferential order, within recent alluvial deposits, the Bakhtiyari Conglomerates, and the Aghajari Sandstone. The Asmari Limestone and other units have much lower potential for groundwater supplies. Potential usefulness of the RSGIS method was indicated when six out of nine producing wells recently drilled by the Khozestan Water and Power Authority (which had no knowledge of this study) were located in areas preferentially selected by this technique.

Depressive Symptoms among Latino Farmworkers across the Agricultural Season: Structural and Situational Influences

PubMed Central

Grzywacz, Joseph G.; Quandt, Sara A.; Chen, Haiying; Isom, Scott; Kiang, Lisa; Vallejos, Quirina; Arcury, Thomas A.

2010-01-01

Immigrant Latino farmworkers confront multiple challenges that threaten their mental health. Previous farmworker mental health research has relied primarily on cross-sectional study designs, leaving little opportunity to describe how farmworker mental health changes or to identify factors that may contribute to these changes. This study used prospective data obtained at monthly intervals across one four-month agricultural season from a large sample of Latino farmworkers in NC (N=288) to document variation in depressive symptoms across the agricultural season and delineate structural and situational factors associated with mental health trajectories across time. Depressive symptoms generally followed a U-shaped distribution across the season, but there was substantial variation in this pattern. Structural stressors like marital status and situational stressors like the pace of work, crowded living conditions, and concerns about documentation predicted depressive symptoms. The pattern of results suggests that strategies to address mental health problems in this vulnerable population will require coordinated action at the individual and social level. PMID:20658876

Regulation of T-cell receptor signalling by membrane microdomains

PubMed Central

Razzaq, Tahir M; Ozegbe, Patricia; Jury, Elizabeth C; Sembi, Phupinder; Blackwell, Nathan M; Kabouridis, Panagiotis S

2004-01-01

There is now considerable evidence suggesting that the plasma membrane of mammalian cells is compartmentalized by functional lipid raft microdomains. These structures are assemblies of specialized lipids and proteins and have been implicated in diverse biological functions. Analysis of their protein content using proteomics and other methods revealed enrichment of signalling proteins, suggesting a role for these domains in intracellular signalling. In T lymphocytes, structure/function experiments and complementary pharmacological studies have shown that raft microdomains control the localization and function of proteins which are components of signalling pathways regulated by the T-cell antigen receptor (TCR). Based on these studies, a model for TCR phosphorylation in lipid rafts is presented. However, despite substantial progress in the field, critical questions remain. For example, it is unclear if membrane rafts represent a homogeneous population and if their structure is modified upon TCR stimulation. In the future, proteomics and the parallel development of complementary analytical methods will undoubtedly contribute in further delineating the role of lipid rafts in signal transduction mechanisms. PMID:15554919

Depressive symptoms among Latino farmworkers across the agricultural season: Structural and situational influences.

PubMed

Grzywacz, Joseph G; Quandt, Sara A; Chen, Haiying; Isom, Scott; Kiang, Lisa; Vallejos, Quirina; Arcury, Thomas A

2010-07-01

Immigrant Latino farmworkers confront multiple challenges that threaten their mental health. Previous farmworker mental health research has relied primarily on cross-sectional study designs, leaving little opportunity to describe how farmworker mental health changes or to identify factors that may contribute to these changes. This study used prospective data obtained at monthly intervals across one 4-month agricultural season from a large sample of Latino farmworkers in North Carolina (N = 288) to document variation in depressive symptoms across the agricultural season and delineate structural and situational factors associated with mental health trajectories across time. Depressive symptoms generally followed a U-shaped distribution across the season, but there was substantial variation in this pattern. Structural stressors like marital status and situational stressors like the pace of work, crowded living conditions, and concerns about documentation predicted depressive symptoms. The pattern of results suggests that strategies to address mental health problems in this vulnerable population will require coordinated action at the individual and social level.

Dosimetric impact in the dose-volume histograms of rectal and vesical wall contouring in prostate cancer IMRT treatments.

PubMed

Gómez, Laura; Andrés, Carlos; Ruiz, Antonio

2017-01-01

The main purpose of this study was to evaluate the differences in dose-volume histograms of IMRT treatments for prostate cancer based on the delineation of the main organs at risk (rectum and bladder) as solid organs or by contouring their wall. Rectum and bladder have typically been delineated as solid organs, including the waste material, which, in practice, can lead to an erroneous assessment of the risk of adverse effects. A retrospective study was made on 25 patients treated with IMRT radiotherapy for prostate adenocarcinoma. 76.32 Gy in 36 fractions was prescribed to the prostate and seminal vesicles. In addition to the delineation of the rectum and bladder as solid organs (including their content), the rectal and bladder wall were also delineated and the resulting dose-volume histograms were analyzed for the two groups of structures. Data analysis shows statistically significant differences in the main parameters used to assess the risk of toxicity of a prostate radiotherapy treatment. Higher doses were received on the rectal and bladder walls compared to doses received on the corresponding solid organs. The observed differences in terms of received doses to the rectum and bladder based on the method of contouring could gain greater importance in inverse planning treatments, where the treatment planning system optimizes the dose in these volumes. So, one should take into account the method of delineating of these structures to make a clinical decision regarding dose limitation and risk assessment of chronic toxicity.

Incremental Value of Magnetic Resonance Imaging in Further Characterizing Hypodense Mediastinal and Paracardiac Lesions Identified on Computed Tomography

PubMed Central

Chaturvedi, Abhishek; Gange, Chris; Sahin, Hakan; Chaturvedi, Apeksha

2018-01-01

Mediastinal and paracardiac lesions are usually first diagnosed on a chest radiograph or echocardiogram. Often, a computed tomography is obtained to further delineate these lesions. CT may be suboptimal for evaluation of enhancement characteristics and direct extension into the adjacent mediastinal structures. With its intrinsic superior soft-tissue characterization, magnetic resonance imaging (MRI) can better delineate these lesions, their internal tissue characteristics, and identify adhesion/invasion into adjacent structures. This pictorial essay provides a brief synopsis of the key MRI sequences and their utility in further characterizing mediastinal and paracardiac lesions. PMID:29619281

Management of the Space Physics Analysis Network (SPAN)

NASA Technical Reports Server (NTRS)

Green, James L.; Thomas, Valerie L.; Butler, Todd F.; Peters, David J.; Sisson, Patricia L.

1990-01-01

Here, the purpose is to define the operational management structure and to delineate the responsibilities of key Space Physics Analysis Network (SPAN) individuals. The management structure must take into account the large NASA and ESA science research community by giving them a major voice in the operation of the system. Appropriate NASA and ESA interfaces must be provided so that there will be adequate communications facilities available when needed. Responsibilities are delineated for the Advisory Committee, the Steering Committee, the Project Scientist, the Project Manager, the SPAN Security Manager, the Internetwork Manager, the Network Operations Manager, the Remote Site Manager, and others.

Participatory action research in corrections: The HITEC 2 program.

PubMed

Cherniack, Martin; Dussetschleger, Jeffrey; Dugan, Alicia; Farr, Dana; Namazi, Sara; El Ghaziri, Mazen; Henning, Robert

2016-03-01

HITEC 2 (Health Improvement through Employee Control 2) is the follow-up to HITEC, a participatory action research (PAR) program that integrates health and work conditions interventions designed by the workforce. HITEC 2 compares intervention programs between two correctional sites, one using a pure workforce level design team and the other using a more structured and time delineated labor-management kaizen effectiveness team. HITEC 2 utilizes a seven step participatory Intervention Design and Analysis Scorecard (IDEAS) for planning interventions. Consistent with PAR, process and intervention efficacy measures are developed and administered through workforce representation. Participation levels, robustness of participatory structures and sophistication of interventions have increased at each measured interval. Health comparisons between 2008 and 2013 showed increased hypertension, static weight maintenance, and increased 'readiness to change'. The PAR approaches are robust and sustained. Their long-term effectiveness in this population is not yet clear. Copyright © 2015 Elsevier Ltd and The Ergonomics Society. All rights reserved.

Population Genomics of the Euryhaline Teleost Poecilia latipinna

PubMed Central

Hoover, D.; Travis, J.; Oleksiak, M. F.; Crawford, D. L.

2015-01-01

Global climate change and increases in sea levels will affect coastal marine communities. The conservation of these ecologically important areas will be a challenge because of their wide geographic distribution, ecological diversity and species richness. To address this problem, we need to better understand how the genetic variation of the species in these communities is distributed within local populations, among populations and between distant regions. In this study we apply genotyping by sequencing (GBS) and examine 955 SNPs to determine Sailfin molly (Poecilia latipinna) genetic diversity among three geographically close mangrove salt marsh flats in the Florida Keys compared to populations in southern and northern Florida. The questions we are asking are whether there is sufficient genetic variation among isolated estuarine fish within populations and whether there are significant divergences among populations. Additionally, we want to know if GBS approaches agree with previous studies using more traditional molecular approaches. We are able to identify large genetic diversity within each saltmarsh community (π ≈ 36%). Additionally, among the Florida Key populations and the mainland or between southern and northern Florida regions, there are significant differences in allele frequencies seen in population structure and evolutionary relationships among individuals. Surprisingly, even though the cumulative FST value using all 955 SNPs within the three Florida Key populations is small, there are 29 loci with significant FST values, and 11 of these were outliers suggestive of adaptive divergence. These data suggest that among the salt marsh flats surveyed here, there is significant genetic diversity within each population and small but significant differences among populations. Much of the genetic variation within and among populations found here with GBS is very similar to previous studies using allozymes and microsatellites. However, the meaningful difference between GBS and these previous measures of genetic diversity is the number of loci examined, which allows more precise delineations of population structure as well as facilitates identifying loci with excessive FST values that could indicate adaptive divergence. PMID:26335684

Population Genomics of the Euryhaline Teleost Poecilia latipinna.

PubMed

Nunez, J C B; Seale, T P; Fraser, M A; Burton, T L; Fortson, T N; Hoover, D; Travis, J; Oleksiak, M F; Crawford, D L

2015-01-01

Global climate change and increases in sea levels will affect coastal marine communities. The conservation of these ecologically important areas will be a challenge because of their wide geographic distribution, ecological diversity and species richness. To address this problem, we need to better understand how the genetic variation of the species in these communities is distributed within local populations, among populations and between distant regions. In this study we apply genotyping by sequencing (GBS) and examine 955 SNPs to determine Sailfin molly (Poecilia latipinna) genetic diversity among three geographically close mangrove salt marsh flats in the Florida Keys compared to populations in southern and northern Florida. The questions we are asking are whether there is sufficient genetic variation among isolated estuarine fish within populations and whether there are significant divergences among populations. Additionally, we want to know if GBS approaches agree with previous studies using more traditional molecular approaches. We are able to identify large genetic diversity within each saltmarsh community (π ≈ 36%). Additionally, among the Florida Key populations and the mainland or between southern and northern Florida regions, there are significant differences in allele frequencies seen in population structure and evolutionary relationships among individuals. Surprisingly, even though the cumulative FST value using all 955 SNPs within the three Florida Key populations is small, there are 29 loci with significant FST values, and 11 of these were outliers suggestive of adaptive divergence. These data suggest that among the salt marsh flats surveyed here, there is significant genetic diversity within each population and small but significant differences among populations. Much of the genetic variation within and among populations found here with GBS is very similar to previous studies using allozymes and microsatellites. However, the meaningful difference between GBS and these previous measures of genetic diversity is the number of loci examined, which allows more precise delineations of population structure as well as facilitates identifying loci with excessive FST values that could indicate adaptive divergence.

Oak decline in the Boston Mountains, Arkansas, USA: Spatial and temporal patterns under two fire regimes

Treesearch

Martin A. Spetich; Hong S. He

2008-01-01

A spatially explicit forest succession and disturbance model is used to delineate the extent and dispersion of oak decline under two fire regimes over a 150-year period. The objectives of this study are to delineate potential current and future oak decline areas using species composition and age structure data in combination with ecological land types, and to...

Ancient Humans Influenced the Current Spatial Genetic Structure of Common Walnut Populations in Asia

PubMed Central

Pollegioni, Paola; Woeste, Keith E.; Chiocchini, Francesca; Del Lungo, Stefano; Olimpieri, Irene; Tortolano, Virginia; Clark, Jo; Hemery, Gabriel E.; Mapelli, Sergio; Malvolti, Maria Emilia

2015-01-01

Common walnut (Juglans regia L) is an economically important species cultivated worldwide for its wood and nuts. It is generally accepted that J. regia survived and grew spontaneously in almost completely isolated stands in its Asian native range after the Last Glacial Maximum. Despite its natural geographic isolation, J. regia evolved over many centuries under the influence of human management and exploitation. We evaluated the hypothesis that the current distribution of natural genetic resources of common walnut in Asia is, at least in part, the product of ancient anthropogenic dispersal, human cultural interactions, and afforestation. Genetic analysis combined with ethno-linguistic and historical data indicated that ancient trade routes such as the Persian Royal Road and Silk Road enabled long-distance dispersal of J. regia from Iran and Trans-Caucasus to Central Asia, and from Western to Eastern China. Ancient commerce also disrupted the local spatial genetic structure of autochthonous walnut populations between Tashkent and Samarkand (Central-Eastern Uzbekistan), where the northern and central routes of the Northern Silk Road converged. A significant association between ancient language phyla and the genetic structure of walnut populations is reported even after adjustment for geographic distances that could have affected both walnut gene flow and human commerce over the centuries. Beyond the economic importance of common walnut, our study delineates an alternative approach for understanding how the genetic resources of long-lived perennial tree species may be affected by the interaction of geography and human history. PMID:26332919

Fast automatic delineation of cardiac volume of interest in MSCT images

NASA Astrophysics Data System (ADS)

Lorenz, Cristian; Lessick, Jonathan; Lavi, Guy; Bulow, Thomas; Renisch, Steffen

2004-05-01

Computed Tomography Angiography (CTA) is an emerging modality for assessing cardiac anatomy. The delineation of the cardiac volume of interest (VOI) is a pre-processing step for subsequent visualization or image processing. It serves the suppression of anatomic structures being not in the primary focus of the cardiac application, such as sternum, ribs, spinal column, descending aorta and pulmonary vasculature. These structures obliterate standard visualizations such as direct volume renderings or maximum intensity projections. In addition, outcome and performance of post-processing steps such as ventricle suppression, coronary artery segmentation or the detection of short and long axes of the heart can be improved. The structures being part of the cardiac VOI (coronary arteries and veins, myocardium, ventricles and atria) differ tremendously in appearance. In addition, there is no clear image feature associated with the contour (or better cut-surface) distinguishing between cardiac VOI and surrounding tissue making the automatic delineation of the cardiac VOI a difficult task. The presented approach locates in a first step chest wall and descending aorta in all image slices giving a rough estimate of the location of the heart. In a second step, a Fourier based active contour approach delineates slice-wise the border of the cardiac VOI. The algorithm has been evaluated on 41 multi-slice CT data-sets including cases with coronary stents and venous and arterial bypasses. The typical processing time amounts to 5-10s on a 1GHz P3 PC.

In vivo delineation of subdivisions of the human amygdaloid complex in a high-resolution group template

PubMed Central

Tyszka, J. Michael; Pauli, Wolfgang M.

2016-01-01

The nuclei of the human amygdala remain difficult to distinguish in individual subject structural magnetic resonance images. However, interpretation of the amygdala’s role in whole brain networks requires accurate localization of functional activity to a particular nucleus or subgroup of nuclei. To address this, we constructed high spatial resolution, three-dimensional templates, using joint high accuracy diffeomorphic registration of T1- and T2-weighted structural images from 168 typical adults between 22 and 35 years old released by the Human Connectome Project. Several internuclear boundaries are clearly visible in these templates, which would otherwise be impossible to delineate in individual subject data. A probabilistic atlas of major nuclei and nuclear groups was constructed in this template space and mapped back to individual spaces by inversion of the individual diffeomorphisms. Group level analyses revealed a slight (approximately 2%) bias towards larger total amygdala and nuclear volumes in the right hemisphere. No substantial sex or age differences were found in amygdala volumes normalized to total intracranial volume, or subdivision volumes normalized to amygdala volume. The current delineation provides a finer parcellation of the amygdala with more accurate external boundary definition than current histology-based atlases when used in conjunction with high accuracy registration methods, such as diffeomorphic warping. These templates and delineation are intended to be an open and evolving resource for future functional and structural imaging studies of the human amygdala. PMID:27354150

SU-E-J-275: Impact of the Intra and Inter Observer Variability in the Delineation of Parotid Glands On the Dose Calculation During Head and Neck Helical Tomotherapy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jodda, A; Piotrowski, T

2014-06-01

Purpose: The intra- and inter-observer variability in delineation of the parotids on the kilo-voltage computed tomography (kVCT) and mega-voltage computed tomography (MVCT) were examined to establish their impact on the dose calculation during adaptive head and neck helical tomotherapy (HT). Methods: Three observers delineated left and right parotids for ten randomly selected patients with oropharynx cancer treated on HT. The pre-treatment kVCT and the MVCT from the first fraction of irradiation were selected to delineation. The delineation procedure was repeated three times by each observer. The parotids were delineated according to the institutional protocol. The analyses included intra-observer reproducibility andmore » inter-structure, -observer and -modality variability of the volume and dose. Results: The differences between the left and right parotid outlines were not statistically significant (p>0.3). The reproducibility of the delineation was confirmed for each observer on the kVCT (p>0.2) and on the MVCT (p>0.1). The inter-observer variability of the outlines was significant (p<0.001) as well as the inter-modality variability (p<0.006). The parotids delineated on the MVCT were 10% smaller than on the kVCT. The inter-observer variability of the parotids delineation did not affect the average dose (p=0.096 on the kVCT and p=0.176 on the MVCT). The dose calculated on the MVCT was higher by 3.3% than dose from the kVCT (p=0.009). Conclusion: Usage of the institutional protocols for the parotids delineation reduces intra-observer variability and increases reproducibility of the outlines. These protocols do not eliminate delineation differences between the observers, but these differences are not clinically significant and do not affect average doses in the parotids. The volumes of the parotids delineated on the MVCT are smaller than on the kVCT, which affects the differences in the calculated doses.« less

Toward an Ideal Senior High School Governance Structure.

ERIC Educational Resources Information Center

Treslan, D. L.

1979-01-01

This paper delineates six attributes of an ideal high school governance structure: respect, freedom, rationality, flexibility, equality, and involvement of staff and students in the decision-making process. (Author/SJL)

Flying over an infected landscape: Distribution of highly pathogenic avian influenza H5N1 risk in South Asia and satellite tracking of wild waterfowl

USGS Publications Warehouse

Gilbert, Marius; Newman, Scott H.; Takekawa, John Y.; Loth, Leo; Biradar, Chandrashekhar; Prosser, Diann J.; Balachandran, Sivananinthaperumal; Rao, Mandava Venkata Subba; Mundkur, Taej; Yan, Baoping; Xing, Zhi; Hou, Yuansheng; Batbayar, Nyambayar; Tseveenmayadag, Natsagdorj; Hogerwerf, Lenny; Slingenbergh, Jan; Xiao, Xiangming

2010-01-01

Highly pathogenic avian influenza (HPAI) H5N1 virus persists in Asia, posing a threat to poultry, wild birds, and humans. Previous work in Southeast Asia demonstrated that HPAI H5N1 risk is related to domestic ducks and people. Other studies discussed the role of migratory birds in the long distance spread of HPAI H5N1. However, the interplay between local persistence and long-distance dispersal has never been studied. We expand previous geospatial risk analysis to include South and Southeast Asia, and integrate the analysis with migration data of satellite-tracked wild waterfowl along the Central Asia flyway. We find that the population of domestic duck is the main factor delineating areas at risk of HPAI H5N1 spread in domestic poultry in South Asia, and that other risk factors, such as human population and chicken density, are associated with HPAI H5N1 risk within those areas. We also find that satellite tracked birds (Ruddy Shelduck and two Bar-headed Geese) reveal a direct spatio-temporal link between the HPAI H5N1 hot-spots identified in India and Bangladesh through our risk model, and the wild bird outbreaks in May,June,July 2009 in China(Qinghai Lake), Mongolia, and Russia. This suggests that the continental-scale dynamics of HPAI H5N1 are structured as a number of persistence areas delineated by domestic ducks, connected by rare transmission through migratory waterfowl.

Phylogenetic information in polymorphic L1 and Alu insertions from East Asians and Native American populations.

PubMed

Mateus Pereira, L H; Socorro, A; Fernandez, I; Masleh, M; Vidal, D; Bianchi, N O; Bonatto, S L; Salzano, F M; Herrera, R J

2005-09-01

This study attempts to ascertain genetic affinities between Native American and East Asian populations by analyzing four polymorphic Alu insertions (PAIs) and three L1 polymorphic loci. These two genetic systems demonstrated strong congruence when levels of diversity and genetic distances were considered. Overall, genetic relatedness within Native American groups does not correlate with geographical and linguistic structure, although strong grouping for Native Americans with East Asians was demonstrated, with clear discrimination from African and European groups. Most of the variation was assigned to differences occurring within groups, but the interpopulation variation found for South Amerindians was recognizably higher in comparison to the other sampled groups of populations. Our data suggest that bottleneck events followed by strong influence of genetic drift in the process of the peopling of the Americas may have been determinant factors in delineating the genetic background of present-day South Amerindians. Since no clear subgroups were detected within Native Americans and East Asians, there is no indication of multiple waves in the early colonization of the New World. (c) 2005 Wiley-Liss, Inc.

Tools for assessing kinship, population structure, phylogeography, and interspecific hybridization in Asian carps invasive to the Mississippi River, USA: isolation and characterization of novel tetranucleotide microsatellite DNA loci in silver carp Hypophthalmichthys molitrix

USGS Publications Warehouse

King, T.L.; Eackles, M.S.; Chapman, D.C.

2011-01-01

We document the isolation and characterization of novel tetranucleotide microsatellite DNA markers for the invasive silver carp Hypophthalmichthys molitrix and provide the results of cross-species amplification for three additional invasive carp species: bighead (H. nobilis), grass (Ctenopharyngodon idella) and black (Mylopharyngodon piceus). In the target species these markers yielded levels of allelic diversity (average 4.4 alleles/locus) and heterozygosity (average 54.7%) sufficient to: (1) provide unique multilocus genotypes; (2) delineate kinship relationships; (3) differentiate populations/species; (4) estimate effective population sizes; and (5) provide unique demographic perspectives for control or eradication. Currently these markers are being utilized to determine the degree of introgressive hybridization between H. molitrix and H. nobilis, to quantify gene flow between different sub-basins established in the central United States, and to assess the demographic status of sub-basin groups. This information will be critically important in the management/control of these invasive species.

Beyond Population Distribution: Enhancing Sociocultural Resolution from Remote Sensing

NASA Astrophysics Data System (ADS)

Bhaduri, B. L.; Rose, A.

2017-12-01

At Oak Ridge National Laboratory, since late 1990s, we have focused on developing high resolution population distribution and dynamics data from local to global scales. Increasing resolutions of geographic data has been mirrored by population data sets developed across the community. However, attempts to increase temporal and sociocultural resolutions have been limited given the lack of high resolution data on human settlements and activities. While recent advancements in moderate to high resolution earth observation have led to better physiographic data, the approach of exploiting very high resolution (sub-meter resolution) imagery has also proven useful for generating accurate human settlement maps. It allows potential (social and vulnerability) characterization of population from settlement structures by exploiting image texture and spectral features. Our recent research utilizing machine learning and geocomputation has not only validated "poverty mapping from imagery" hypothesis, but has delineated a new paradigm of rapid analysis of high resolution imagery to enhance such "neighborhood" mapping techniques. Such progress in GIScience is allowing us to move towards the goal of creating a global foundation level database for impervious surfaces and "neighborhoods," and holds tremendous promise for key applications focusing on sustainable development including many social science applications.

Digital database architecture and delineation methodology for deriving drainage basins, and a comparison of digitally and non-digitally derived numeric drainage areas

USGS Publications Warehouse

Dupree, Jean A.; Crowfoot, Richard M.

2012-01-01

The drainage basin is a fundamental hydrologic entity used for studies of surface-water resources and during planning of water-related projects. Numeric drainage areas published by the U.S. Geological Survey water science centers in Annual Water Data Reports and on the National Water Information Systems (NWIS) Web site are still primarily derived from hard-copy sources and by manual delineation of polygonal basin areas on paper topographic map sheets. To expedite numeric drainage area determinations, the Colorado Water Science Center developed a digital database structure and a delineation methodology based on the hydrologic unit boundaries in the National Watershed Boundary Dataset. This report describes the digital database architecture and delineation methodology and also presents the results of a comparison of the numeric drainage areas derived using this digital methodology with those derived using traditional, non-digital methods. (Please see report for full Abstract)

A simplified CT-based definition of the supraclavicular and infraclavicular nodal volumes in breast cancer.

PubMed

Atean, I; Pointreau, Y; Ouldamer, L; Monghal, C; Bougnoux, A; Bera, G; Barillot, I

2013-02-01

The available contouring guidelines for the supraclavicular and infraclavicular lymph nodes appeared to be inadequate for their delineation on non-enhanced computed tomography (CT) scans. For this purpose, we developed delineation guidelines for the clinical target volumes (CTV) of these lymph nodes on non-enhanced CT-slices performed in the treatment position of breast cancer. A fresh female cadaver study as well as delineation and an anatomical descriptions review were performed to propose a simplified definition of the supra- and infraclavicular lymph nodes using readily identifiable anatomical structures. This definition was developed jointly by breast radiologists, breast surgeons, and radiation oncologists. To validate these guidelines, the primary investigator and seven radiation oncologists (observers) independently delineated 10 different nodal CTVs. The primary investigator contours were considered to be the gold standard contours. Contour accuracy and concordance were evaluated. Written guidelines for the delineation of supra- and infraclavicular lymph nodes CTVs were developed. Consistent contours with minimal variability existed between the delineated volumes; the mean kappa index was 0.83. The mean common contoured and additional contoured volumes were 84.6% and 18.5%, respectively. The mean overlap volume ratio was 0.71. Simplified CT-based atlas for delineation of the supra- and infraclavicular lymph nodes for locoregional irradiation of the breast on non-enhanced CT-scan, have been developed in this study. This atlas provides a consistent set of guidelines for delineating these volumes. Copyright © 2012 Société française de radiothérapie oncologique (SFRO). Published by Elsevier SAS. All rights reserved.

Earth retaining structures manual

DOT National Transportation Integrated Search

2009-10-29

The objectives of this policy are to obtain statewide uniformity, establish standard : procedures and delineate responsibility for the preparation and review of plans, : design and construction control of earth retaining structures. In addition, it i...

New summer areas and mixing of two greater sandhill crane populations in the Intermountain West

USGS Publications Warehouse

Collins, Daniel P.; Grisham, Blake A.; Conring, Courtenay M.; Knetter, Jeffrey M.; Conway, Warren C.; Carleton, Scott A.; Boggie, Matthew A.

2016-01-01

Population delineation throughout the annual life cycle for migratory birds is needed to formulate regional and national management and conservation strategies. Despite being well studied continentally, connectivity of sandhill crane Grus canadensis populations throughout the western portion of their North American range remains poorly described. Our objectives were to 1) use global positioning system satellite transmitter terminals to identify summer distributions for the Lower Colorado River Valley Population of greater sandhill cranes Grus canadensis tabida and 2) determine whether intermingling occurs among any of the western greater sandhill crane populations: Rocky Mountain Population, Lower Colorado River Valley Population, and Central Valley Population. Capture and marking occurred during winter and summer on private lands in California and Idaho as well as on two National Wildlife Refuges: Cibola and Sonny Bono Salton Sea National Wildlife Refuges. A majority of marked greater sandhill cranes summered in what is established Lower Colorado River Valley Population breeding areas in northeastern Nevada and southwestern Idaho. A handful of greater sandhill cranes summered outside of traditional breeding areas in west-central Idaho around Cascade Reservoir near Donnelly and Cascade, Idaho. For example, a greater sandhill crane colt captured near Donnelly in July 2014 survived to winter migration and moved south to areas associated with the Rocky Mountain Population. The integration of the greater sandhill crane colt captured near Donnelly provides the first evidence of potential intermingling between the Lower Colorado River Population and Rocky Mountain Population. We suggest continued marking and banding efforts of all three western populations of greater sandhill cranes will accurately delineate population boundaries and connectivity and inform management decisions for the three populations.

Cortical Enhanced Tissue Segmentation of Neonatal Brain MR Images Acquired by a Dedicated Phased Array Coil

PubMed Central

Shi, Feng; Yap, Pew-Thian; Fan, Yong; Cheng, Jie-Zhi; Wald, Lawrence L.; Gerig, Guido; Lin, Weili; Shen, Dinggang

2010-01-01

The acquisition of high quality MR images of neonatal brains is largely hampered by their characteristically small head size and low tissue contrast. As a result, subsequent image processing and analysis, especially for brain tissue segmentation, are often hindered. To overcome this problem, a dedicated phased array neonatal head coil is utilized to improve MR image quality by effectively combing images obtained from 8 coil elements without lengthening data acquisition time. In addition, a subject-specific atlas based tissue segmentation algorithm is specifically developed for the delineation of fine structures in the acquired neonatal brain MR images. The proposed tissue segmentation method first enhances the sheet-like cortical gray matter (GM) structures in neonatal images with a Hessian filter for generation of cortical GM prior. Then, the prior is combined with our neonatal population atlas to form a cortical enhanced hybrid atlas, which we refer to as the subject-specific atlas. Various experiments are conducted to compare the proposed method with manual segmentation results, as well as with additional two population atlas based segmentation methods. Results show that the proposed method is capable of segmenting the neonatal brain with the highest accuracy, compared to other two methods. PMID:20862268

ESTRO ACROP guidelines for target volume definition in the treatment of locally advanced non-small cell lung cancer.

PubMed

Nestle, Ursula; De Ruysscher, Dirk; Ricardi, Umberto; Geets, Xavier; Belderbos, Jose; Pöttgen, Christoph; Dziadiuszko, Rafal; Peeters, Stephanie; Lievens, Yolande; Hurkmans, Coen; Slotman, Ben; Ramella, Sara; Faivre-Finn, Corinne; McDonald, Fiona; Manapov, Farkhad; Putora, Paul Martin; LePéchoux, Cécile; Van Houtte, Paul

2018-04-01

Radiotherapy (RT) plays a major role in the curative treatment of locally advanced non-small cell lung cancer (NSCLC). Therefore, the ACROP committee was asked by the ESTRO to provide recommendations on target volume delineation for standard clinical scenarios in definitive (chemo)radiotherapy (RT) and adjuvant RT for locally advanced NSCLC. The guidelines given here are a result of the evaluation of a structured questionnaire followed by a consensus discussion, voting and writing procedure within the committee. Hence, we provide advice for methods and time-points of diagnostics and imaging before the start of treatment planning and for the mandatory and optional imaging to be used for planning itself. Concerning target volumes, recommendations are given for GTV delineation of primary tumour and lymph nodes followed by issues related to the delineation of CTVs for definitive and adjuvant radiotherapy. In the context of PTV delineation, recommendations about the management of geometric uncertainties and target motion are given. We further provide our opinions on normal tissue delineation and organisational and responsibility questions in the process of target volume delineation. This guideline intends to contribute to the standardisation and optimisation of the process of RT treatment planning for clinical practice and prospective studies. Copyright © 2018 Elsevier B.V. All rights reserved.

Congenital amusia.

PubMed

Williamson, Victoria J; Stewart, Lauren

2013-01-01

For most people, music, like language, is acquired effortlessly in early life. But a few percent of the population have lifelong difficulties in the perception and production of music. In this chapter we discuss psycho-acoustic and behavioral studies that have attempted to delineate the nature of the auditory perceptual deficits in this group and consider whether these difficulties extend outside the musical domain. Finally, we review structural imaging studies in this group which point to subtle anomalies in temporal and frontal areas. We suggest that amusia can be considered a disorder of neural development, which has relatively specific consequences at the behavioral level. Studies of congenital amusia provide a unique window on the neurocognitive architecture of music processing. Copyright © 2013 Elsevier B.V. All rights reserved.

A Profile of Selected Demographic and Socioeconomic Characteristics of Rural Georgia Counties.

ERIC Educational Resources Information Center

Pieper, Hanns; Miller, H. Max

Demographic and selected socioeconomic changes which have occurred during the past decade in rural Georgia are delineated in this paper. The sample consisted of 50 counties, none of which had any urban population in 1960 or 1970. These counties, which are representative of rural regions which are losing or gaining population, were divided into 2…

Enhanced delineation of degradation in aortic walls through OCT

NASA Astrophysics Data System (ADS)

Real, Eusebio; Val-Bernal, José Fernando; Revuelta, José M.; Pontón, Alejandro; Calvo Díez, Marta; Mayorga, Marta; López-Higuera, José M.; Conde, Olga M.

2015-03-01

Degradation of the wall of human ascending thoracic aorta has been assessed through Optical Coherence Tomography (OCT). OCT images of the media layer of the aortic wall exhibit micro-structure degradation in case of diseased aortas from aneurysmal vessels or in aortas prone to aortic dissections. The degeneration in vessel walls appears as low-reflectivity areas due to the invasive appearance of acidic polysaccharides and mucopolysaccharides within a typical ordered microstructure of parallel lamellae of smooth muscle cells, elastin and collagen fibers. An OCT indicator of wall degradation can be generated upon the spatial quantification of the extension of degraded areas in a similar way as conventional histopathology. This proposed OCT marker offers a real-time clinical insight of the vessel status to help cardiovascular surgeons in vessel repair interventions. However, the delineation of degraded areas on the B-scan image from OCT is sometimes difficult due to presence of speckle noise, variable SNR conditions on the measurement process, etc. Degraded areas could be outlined by basic thresholding techniques taking advantage of disorders evidences in B-scan images, but this delineation is not always optimum and requires complex additional processing stages. This work proposes an optimized delineation of degraded spots in vessel walls, robust to noisy environments, based on the analysis of the second order variation of image intensity of backreflection to determine the type of local structure. Results improve the delineation of wall anomalies providing a deeper physiological perception of the vessel wall conditions. Achievements could be also transferred to other clinical scenarios: carotid arteries, aorto-iliac or ilio-femoral sections, intracranial, etc.

Iterative Otsu's method for OCT improved delineation in the aorta wall

NASA Astrophysics Data System (ADS)

Alonso, Daniel; Real, Eusebio; Val-Bernal, José F.; Revuelta, José M.; Pontón, Alejandro; Calvo Díez, Marta; Mayorga, Marta; López-Higuera, José M.; Conde, Olga M.

2015-07-01

Degradation of human ascending thoracic aorta has been visualized with Optical Coherence Tomography (OCT). OCT images of the vessel wall exhibit structural degradation in the media layer of the artery, being this disorder the final trigger of the pathology. The degeneration in the vessel wall appears as low-reflectivity areas due to different optical properties of acidic polysaccharides and mucopolysaccharides in contrast with typical ordered structure of smooth muscle cells, elastin and collagen fibers. An OCT dimension indicator of wall degradation can be generated upon the spatial quantification of the extension of degraded areas in a similar way as conventional histopathology. This proposed OCT marker can offer in the future a real-time clinical perception of the vessel status to help cardiovascular surgeons in vessel repair interventions. However, the delineation of degraded areas on the B-scan image from OCT is sometimes difficult due to presence of speckle noise, variable signal to noise ratio (SNR) conditions on the measurement process, etc. Degraded areas can be delimited by basic thresholding techniques taking advantage of disorders evidences in B-scan images, but this delineation is not optimum in the aorta samples and requires complex additional processing stages. This work proposes an optimized delineation of degraded areas within the aorta wall, robust to noisy environments, based on the iterative application of Otsu's thresholding method. Results improve the delineation of wall anomalies compared with the simple application of the algorithm. Achievements could be also transferred to other clinical scenarios: carotid arteries, aorto-iliac or ilio-femoral sections, intracranial, etc.

Estimating changes in urban land and urban population using refined areal interpolation techniques

NASA Astrophysics Data System (ADS)

Zoraghein, Hamidreza; Leyk, Stefan

2018-05-01

The analysis of changes in urban land and population is important because the majority of future population growth will take place in urban areas. U.S. Census historically classifies urban land using population density and various land-use criteria. This study analyzes the reliability of census-defined urban lands for delineating the spatial distribution of urban population and estimating its changes over time. To overcome the problem of incompatible enumeration units between censuses, regular areal interpolation methods including Areal Weighting (AW) and Target Density Weighting (TDW), with and without spatial refinement, are implemented. The goal in this study is to estimate urban population in Massachusetts in 1990 and 2000 (source zones), within tract boundaries of the 2010 census (target zones), respectively, to create a consistent time series of comparable urban population estimates from 1990 to 2010. Spatial refinement is done using ancillary variables such as census-defined urban areas, the National Land Cover Database (NLCD) and the Global Human Settlement Layer (GHSL) as well as different combinations of them. The study results suggest that census-defined urban areas alone are not necessarily the most meaningful delineation of urban land. Instead, it appears that alternative combinations of the above-mentioned ancillary variables can better depict the spatial distribution of urban land, and thus make it possible to reduce the estimation error in transferring the urban population from source zones to target zones when running spatially-refined temporal areal interpolation.

Changing Urbania: Estimating Changes in Urban Land and Urban Population Using Refined Areal Interpolation Techniques

NASA Astrophysics Data System (ADS)

Zoraghein, H.; Leyk, S.; Balk, D.

2017-12-01

The analysis of changes in urban land and population is important because the majority of future population growth will take place in urban areas. The U.S. Census historically classifies urban land using population density and various land-use criteria. This study analyzes the reliability of census-defined urban lands for delineating the spatial distribution of urban population and estimating its changes over time. To overcome the problem of incompatible enumeration units between censuses, regular areal interpolation methods including Areal Weighting (AW) and Target Density Weighting (TDW), with and without spatial refinement, are implemented. The goal in this study is to estimate urban population in Massachusetts in 1990 and 2000 (source zones), within tract boundaries of the 2010 census (target zones), respectively, to create a consistent time series of comparable urban population estimates from 1990 to 2010. Spatial refinement is done using ancillary variables such as census-defined urban areas, the National Land Cover Database (NLCD) and the Global Human Settlement Layer (GHSL) as well as different combinations of them. The study results suggest that census-defined urban areas alone are not necessarily the most meaningful delineation of urban land. Instead it appears that alternative combinations of the above-mentioned ancillary variables can better depict the spatial distribution of urban land, and thus make it possible to reduce the estimation error in transferring the urban population from source zones to target zones when running spatially-refined temporal areal interpolation.

Structural Precursors to Identity Processes: The Role of Proximate Social Structures

ERIC Educational Resources Information Center

Merolla, David M.; Serpe, Richard T.; Stryker, Sheldon; Schultz, P. Wesley

2012-01-01

This research investigates how participation in college-based science-training programs increases student intention to pursue a scientific career. Using identity theory, we delineate three levels of social structure and conceptualize science-training programs as proximate social structures. Results from a sample of 892 undergraduate science…

Extensive Clonality and Strong Differentiation in the Insular Pacific Tree Santalum insulare: Implications for its Conservation

PubMed Central

LHUILLIER, EMELINE; BUTAUD, JEAN-FRANÇOIS; BOUVET, JEAN-MARC

2006-01-01

• Background and Aims The impact of evolutionary forces on insular systems is particularly exacerbated by the remoteness of islands, strong founder effects, small population size and the influence of biotic and abiotic factors. Patterns of molecular diversity were analysed in an island system with Santalum insulare, a sandalwood species endemic to eastern Polynesia. The aims were to evaluate clonality and to study the genetic diversity and structure of this species, in order to understand the evolutionary process and to define a conservation strategy. • Methods Eight nuclear microsatellites were used to investigate clonality, genetic variation and structure of the French Polynesian sandalwood populations found on ten islands distributed over three archipelagos. • Key Results It was found that 58 % of the 384 trees analysed were clones. The real size of the populations is thus dramatically reduced, with sometimes only one genet producing ramets by root suckering. The diversity parameters were low for islands (nA = 1·5–5·0; HE = 0·28–0·49). No departure from Hardy–Weinberg proportion was observed except within Tahiti island, where a significant excess of homozygotes was noted in the highland population. Genetic structure was characterized by high levels of differentiation between archipelagos (27 % of the total variation) and islands (FST = 0·50). The neighbour-joining tree did not discriminate the three archipelagos but separated the Society archipelago from the other two. • Conclusions This study shows that clonality is a frequent phenomenon in S. insulare. The genetic diversity within populations is lower than the values assessed in species distributed on the mainland, as a consequence of insularity. But this can also be explained by the overexploitation of sandalwood. The differentiation between archipelagos and islands within archipelagos is very high because of the limited gene flow due to oceanic barriers. Delineation of evolutionary significant units and principles for population management are proposed based on this molecular analysis. PMID:16945947

Global Population Structure of a Worldwide Pest and Virus Vector: Genetic Diversity and Population History of the Bemisia tabaci Sibling Species Group

PubMed Central

2016-01-01

The whitefly Bemisia tabaci sibling species (sibsp.) group comprises morphologically indiscernible lineages of well-known exemplars referred to as biotypes. It is distributed throughout tropical and subtropical latitudes and includes the contemporary invasive haplotypes, termed B and Q. Several well-studied B. tabaci biotypes exhibit ecological and biological diversity, however, most members are poorly studied or completely uncharacterized. Genetic studies have revealed substantial diversity within the group based on a fragment of the mitochondrial cytochrome oxidase I (mtCOI) sequence (haplotypes), with other tested markers being less useful for deep phylogenetic comparisons. The view of global relationships within the B. tabaci sibsp. group is largely derived from this single marker, making assessment of gene flow and genetic structure difficult at the population level. Here, the population structure was explored for B. tabaci in a global context using nuclear data from variable microsatellite markers. Worldwide collections were examined representing most of the available diversity, including known monophagous, polyphagous, invasive, and indigenous haplotypes. Well-characterized biotypes and other related geographic lineages discovered represented highly differentiated genetic clusters with little or no evidence of gene flow. The invasive B and Q biotypes exhibited moderate to high levels of genetic diversity, suggesting that they stemmed from large founding populations that have maintained ancestral variation, despite homogenizing effects, possibly due to human-mediated among-population gene flow. Results of the microsatellite analyses are in general agreement with published mtCOI phylogenies; however, notable conflicts exist between the nuclear and mitochondrial relationships, highlighting the need for a multifaceted approach to delineate the evolutionary history of the group. This study supports the hypothesis that the extant B. tabaci sibsp. group contains ancient genetic entities and highlights the vast cryptic diversity throughout the genome in the group. PMID:27855173

Integration of Remote Sensing and Geophysical Applications for Delineation of Geological Structures: Implication for Water Resources in Egypt

NASA Astrophysics Data System (ADS)

Mohamed, L.; Farag, A. Z. A.

2017-12-01

North African countries struggle with insufficient, polluted, oversubscribed, and increasingly expensive water. This natural water shortage, in addition to the lack of a comprehensive scheme for the identification of new water resources challenge the political settings in north Africa. Groundwater is one of the main water resources and its occurrence is controlled by the structural elements which are still poorly understood. Integration of remote sensing images and geophysical tools enable us to delineate the surface and subsurface structures (i.e. faults, joints and shear zones), identify the role of these structures on groundwater flow and then to define the proper locations for groundwater wells. This approach were applied to three different areas in Egypt; southern Sinai, north eastern Sinai and the Eastern Desert using remote sensing, geophysical and hydrogeological datasets as follows: (1) identification of the spatial and temporal rainfall events using meteorological station data and Tropical Rainfall Measuring Mission data; (2) delineation of major faults and shear zones using ALOS Palsar, Landsat 8 and ASTER images, geological maps and field investigation; (3) generation of a normalized difference ratio image using Envisat radar images before and after the rain events to identify preferential water-channeling discontinuities in the crystalline terrain; (4) analysis of well data and derivations of hydrological parameters; (5) validation of the water-channeling discontinuities using Very Low Frequency, testing the structural elements (pre-delineated by remote sensing data) and their depth using gravity, magnetic and Vertical Electrical Sounding methods; (6) generation of regional groundwater flow and isotopic (18O and 2H) distribution maps for the sedimentary aquifer and an approximation flow map for the crystalline aquifer. The outputs include: (1) a conceptual/physical model for the groundwater flow in fractured crystalline and sedimentary aquifers; (2) locations of suggested new wells in light of the findings.

Looping Genomes: Diagnostic Change and the Genetic Makeup of the Autism Population.

PubMed

Navon, Daniel; Eyal, Gil

2016-03-01

This article builds on Hacking's framework of "dynamic nominalism" to show how knowledge about biological etiology can interact with the "kinds of people" delineated by diagnostic categories in ways that "loop" or modify both over time. The authors use historical materials to show how "geneticization" played a crucial role in binding together autism as a biosocial community and how evidence from genetics research later made an important contribution to the diagnostic expansion of autism. In the second part of the article, the authors draw on quantitative and qualitative analyses of autism rates over time in several rare conditions that are delineated strictly according to genomic mutations in order to demonstrate that these changes in diagnostic practice helped to both increase autism's prevalence and create its enormous genetic heterogeneity. Thus, a looping process that began with geneticization and involved the social effects of genetics research itself transformed the autism population and its genetic makeup.

Using qualitative and quantitative formative research to develop tailored nutrition intervention materials for a diverse low-income audience.

PubMed

Strolla, Leslie O; Gans, Kim M; Risica, Patricia M

2006-08-01

More effective nutrition education to reach low-income and ethnic minority populations is needed. As part of a project to develop a tailored nutrition education intervention to meet the needs of low-income Hispanics and non-Hispanics, complementary, mixed methods of formative research were used to determine specific characteristics of the target population. The aim was to ensure that the full array of nutrition messages would be comprehensive enough to effectively tailor to the level of the individual. Barriers to healthy eating were delineated for three main dietary behaviors (number of items delineated in parentheses): lowering fat (11), increasing fruit (8) and increasing vegetables (6). Information was also collected regarding motivators for healthy eating (5), situational barriers to making healthy choices (4), other nutrition-related interests (8) and typical eating habits and food-related choices of the target audience.

Alterations in the Gut ( Gallus gallus) Microbiota Following the Consumption of Zinc Biofortified Wheat ( Triticum aestivum)-Based Diet.

PubMed

Reed, Spenser; Knez, Marija; Uzan, Atara; Stangoulis, James C R; Glahn, Raymond P; Koren, Omry; Tako, Elad

2018-06-15

The structure and function of cecal microbiota following the consumption of a zinc (Zn) biofortified wheat diet was evaluated in a well-studied animal model of human nutrition ( Gallus gallus) during a six-week efficacy trial. Using 16S rRNA gene sequencing, a significant increase in β- but not α-microbial diversity was observed in the animals receiving the Zn biofortified wheat diet, relative to the control. No significant taxonomic differences were found between the two groups. Linear discriminant analysis revealed a group of metagenomic biomarkers that delineated the Zn replete versus Zn deficient phenotypes, such that enrichment of lactic acid bacteria and concomitant increases in Zn-dependent bacterial metabolic pathways were observed in the Zn biofortified group, and expansion of mucin-degraders and specific bacterial groups able to participate in maintaining host Zn homeostasis were observed in the control group. Additionally, the Ruminococcus genus appeared to be a key player in delineating the Zn replete microbiota from the control group, as it strongly predicts host Zn adequacy. Our data demonstrate that the gut microbiome associated with Zn biofortified wheat ingestion is unique and may influence host Zn status. Microbiota analysis in biofortification trials represents a crucial area for study as Zn biofortified diets are increasingly delivered on a population-wide scale.

Advanced MR Imaging of the Human Nucleus Accumbens--Additional Guiding Tool for Deep Brain Stimulation.

PubMed

Lucas-Neto, Lia; Reimão, Sofia; Oliveira, Edson; Rainha-Campos, Alexandre; Sousa, João; Nunes, Rita G; Gonçalves-Ferreira, António; Campos, Jorge G

2015-07-01

The human nucleus accumbens (Acc) has become a target for deep brain stimulation (DBS) in some neuropsychiatric disorders. Nonetheless, even with the most recent advances in neuroimaging it remains difficult to accurately delineate the Acc and closely related subcortical structures, by conventional MRI sequences. It is our purpose to perform a MRI study of the human Acc and to determine whether there are reliable anatomical landmarks that enable the precise location and identification of the nucleus and its core/shell division. For the Acc identification and delineation, based on anatomical landmarks, T1WI, T1IR and STIR 3T-MR images were acquired in 10 healthy volunteers. Additionally, 32-direction DTI was obtained for Acc segmentation. Seed masks for the Acc were generated with FreeSurfer and probabilistic tractography was performed using FSL. The probability of connectivity between the seed voxels and distinct brain areas was determined and subjected to k-means clustering analysis, defining 2 different regions. With conventional T1WI, the Acc borders are better defined through its surrounding anatomical structures. The DTI color-coded vector maps and IR sequences add further detail in the Acc identification and delineation. Additionally, using probabilistic tractography it is possible to segment the Acc into a core and shell division and establish its structural connectivity with different brain areas. Advanced MRI techniques allow in vivo delineation and segmentation of the human Acc and represent an additional guiding tool in the precise and safe target definition for DBS. © 2015 International Neuromodulation Society.

Genetic structure of Cantharellus formosus populations in a second-growth temperate rain forest of the Pacific Northwest

USGS Publications Warehouse

Redman, Regina S.; Ranson, Judith; Rodriguez, Rusty J.

2006-01-01

Cantharellus formosus growing on the Olympic Peninsula of the Pacific Northwest was sampled from September – November 1995 for genetic analysis. A total of ninety-six basidiomes from five clusters separated from one another by 3 - 25 meters were genetically characterized by PCR analysis of 13 arbitrary loci and rDNA sequences. The number of basidiomes in each cluster varied from 15 to 25 and genetic analysis delineated 15 genets among the clusters. Analysis of variance utilizing thirteen apPCR generated genetic molecular markers and PCR amplification of the ribosomal ITS regions indicated that 81.41% of the genetic variation occurred between clusters and 18.59% within clusters. Proximity of the basidiomes within a cluster was not an indicator of genotypic similarity. The molecular profiles of each cluster were distinct and defined as unique populations containing 2 - 6 genets. The monitoring and analysis of this species through non-lethal sampling and future applications is discussed.

Novel tetra-nucleotide microsatellite DNA markers for assessing the evolutionary genetics and demographics of Northern Snakehead (Channa argus) invading North America

USGS Publications Warehouse

King, Timothy L.; Johnson, Robin L.

2011-01-01

We document the isolation and characterization of 19 tetra-nucleotide microsatellite DNA markers in northern snakehead (Channa argus) fish that recently colonized Meadow Lake, New York City, New York. These markers displayed moderate levels of allelic diversity (averaging 6.8 alleles/locus) and heterozygosity (averaging 74.2%). Demographic analyses suggested that the Meadow Lake collection has not achieved mutation-drift equilibrium. These results were consistent with instances of deviations from Hardy–Weinberg equilibrium and the presence of some linkage disequilibrium. A comparison of individual pair-wise distances suggested the presence of multiple differentiated groups of related individuals. Results of all analyses are consistent with a pattern of multiple, recent introductions. The microsatellite markers developed for C. argus yielded sufficient genetic diversity to potentially: (1) delineate kinship; (2) elucidate fine-scale population structure; (3) define management (eradication) units; (4) estimate dispersal rates; (5) estimate population sizes; and (6) provide unique demographic perspectives of control or eradication effectiveness.

Building Models for the Relationship between Attitudes toward Suicide and Suicidal Behavior: Based on Data from General Population Surveys in Sweden, Norway, and Russia

ERIC Educational Resources Information Center

Renberg, Ellinor Salander; Hjelmeland, Heidi; Koposov, Roman

2008-01-01

Our aim was to build a model delineating the relationship between attitudes toward suicide and suicidal behavior and to assess equivalence by applying the model on data from different countries. Representative samples from the general population were approached in Sweden, Norway, and Russia with the Attitudes Toward Suicide (ATTS) questionnaire.…

SU-D-16A-02: A Novel Methodology for Accurate, Semi-Automated Delineation of Oral Mucosa for Radiation Therapy Dose-Response Studies

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dean, J; Welsh, L; Gulliford, S

Purpose: The significant morbidity caused by radiation-induced acute oral mucositis means that studies aiming to elucidate dose-response relationships in this tissue are a high priority. However, there is currently no standardized method for delineating the mucosal structures within the oral cavity. This report describes the development of a methodology to delineate the oral mucosa accurately on CT scans in a semi-automated manner. Methods: An oral mucosa atlas for automated segmentation was constructed using the RayStation Atlas-Based Segmentation (ABS) module. A radiation oncologist manually delineated the full surface of the oral mucosa on a planning CT scan of a patient receivingmore » radiotherapy (RT) to the head and neck region. A 3mm fixed annulus was added to incorporate the mucosal wall thickness. This structure was saved as an atlas template. ABS followed by model-based segmentation was performed on four further patients sequentially, adding each patient to the atlas. Manual editing of the automatically segmented structure was performed. A dose comparison between these contours and previously used oral cavity volume contours was performed. Results: The new approach was successful in delineating the mucosa, as assessed by an experienced radiation oncologist, when applied to a new series of patients receiving head and neck RT. Reductions in the mean doses obtained when using the new delineation approach, compared with the previously used technique, were demonstrated for all patients (median: 36.0%, range: 25.6% – 39.6%) and were of a magnitude that might be expected to be clinically significant. Differences in the maximum dose that might reasonably be expected to be clinically significant were observed for two patients. Conclusion: The method developed provides a means of obtaining the dose distribution delivered to the oral mucosa more accurately than has previously been achieved. This will enable the acquisition of high quality dosimetric data for use in dose-response studies. We would like to thank the Engineering and Physical Sciences Research Council for funding. We acknowledge support from the NIHR RM/ICR Biomedical Research Centre. RayStatation was used under an evaluation agreement with RaySearch Laboratories AB.« less

Ask Questions to Encourage Questions Asked

ERIC Educational Resources Information Center

belcastro, sarah-marie

2017-01-01

We delineate some types of structured practice (modeling, requests, feedback, and space-making) that help students learn to pose appropriate questions and to initiate exploration of those questions. Developing skills requires practice, so we suggest ways to embed structured practice into existing class sessions. Including structured practice is…

Delineating wetland catchments and modeling hydrologic ...

EPA Pesticide Factsheets

In traditional watershed delineation and topographic modeling, surface depressions are generally treated as spurious features and simply removed from a digital elevation model (DEM) to enforce flow continuity of water across the topographic surface to the watershed outlets. In reality, however, many depressions in the DEM are actual wetland landscape features with seasonal to permanent inundation patterning characterized by nested hierarchical structures and dynamic filling–spilling–merging surface-water hydrological processes. Differentiating and appropriately processing such ecohydrologically meaningful features remains a major technical terrain-processing challenge, particularly as high-resolution spatial data are increasingly used to support modeling and geographic analysis needs. The objectives of this study were to delineate hierarchical wetland catchments and model their hydrologic connectivity using high-resolution lidar data and aerial imagery. The graph-theory-based contour tree method was used to delineate the hierarchical wetland catchments and characterize their geometric and topological properties. Potential hydrologic connectivity between wetlands and streams were simulated using the least-cost-path algorithm. The resulting flow network delineated potential flow paths connecting wetland depressions to each other or to the river network on scales finer than those available through the National Hydrography Dataset. The results demonstrated that

A novel algorithm for delineating wetland depressions and ...

EPA Pesticide Factsheets

In traditional watershed delineation and topographic modeling, surface depressions are generally treated as spurious features and simply removed from a digital elevation model (DEM) to enforce flow continuity of water across the topographic surface to the watershed outlets. In reality, however, many depressions in the DEM are actual wetland landscape features that are seldom fully filled with water. For instance, wetland depressions in the Prairie Pothole Region (PPR) are seasonally to permanently flooded wetlands characterized by nested hierarchical structures with dynamic filling- spilling-merging surface-water hydrological processes. The objectives of this study were to delineate hierarchical wetland catchments and model their hydrologic connectivity using high-resolution LiDAR data and aerial imagery. We proposed a novel algorithm delineate the hierarchical wetland catchments and characterize their geometric and topological properties. Potential hydrologic connectivity between wetlands and streams were simulated using the least-cost path algorithm. The resulting flow network delineated putative temporary or seasonal flow paths connecting wetland depressions to each other or to the river network at scales finer than available through the National Hydrography Dataset. The results demonstrated that our proposed framework is promising for improving overland flow modeling and hydrologic connectivity analysis. Presentation at AWRA Spring Specialty Conference in Sn

The effect of dental artifacts, contrast media, and experience on interobserver contouring variations in head and neck anatomy.

PubMed

O'Daniel, Jennifer C; Rosenthal, David I; Garden, Adam S; Barker, Jerry L; Ahamad, Anesa; Ang, K Kian; Asper, Joshua A; Blanco, Angel I; de Crevoisier, Renaud; Holsinger, F Christopher; Patel, Chirag B; Schwartz, David L; Wang, He; Dong, Lei

2007-04-01

To investigate interobserver variability in the delineation of head-and-neck (H&N) anatomic structures on CT images, including the effects of image artifacts and observer experience. Nine observers (7 radiation oncologists, 1 surgeon, and 1 physician assistant) with varying levels of H&N delineation experience independently contoured H&N gross tumor volumes and critical structures on radiation therapy treatment planning CT images alongside reference diagnostic CT images for 4 patients with oropharynx cancer. Image artifacts from dental fillings partially obstructed 3 images. Differences in the structure volumes, center-of-volume positions, and boundary positions (1 SD) were measured. In-house software created three-dimensional overlap distributions, including all observers. The effects of dental artifacts and observer experience on contouring precision were investigated, and the need for contrast media was assessed. In the absence of artifacts, all 9 participants achieved reasonable precision (1 SD < or =3 mm all boundaries). The structures obscured by dental image artifacts had larger variations when measured by the 3 metrics (1 SD = 8 mm cranial/caudal boundary). Experience improved the interobserver consistency of contouring for structures obscured by artifacts (1 SD = 2 mm cranial/caudal boundary). Interobserver contouring variability for anatomic H&N structures, specifically oropharyngeal gross tumor volumes and parotid glands, was acceptable in the absence of artifacts. Dental artifacts increased the contouring variability, but experienced participants achieved reasonable precision even with artifacts present. With a staging contrast CT image as a reference, delineation on a noncontrast treatment planning CT image can achieve acceptable precision.

Genomic insights from whole genome sequencing of four clonal outbreak Campylobacter jejuni assessed within the global C. jejuni population.

PubMed

Clark, Clifford G; Berry, Chrystal; Walker, Matthew; Petkau, Aaron; Barker, Dillon O R; Guan, Cai; Reimer, Aleisha; Taboada, Eduardo N

2016-12-03

Whole genome sequencing (WGS) is useful for determining clusters of human cases, investigating outbreaks, and defining the population genetics of bacteria. It also provides information about other aspects of bacterial biology, including classical typing results, virulence, and adaptive strategies of the organism. Cell culture invasion and protein expression patterns of four related multilocus sequence type 21 (ST21) C. jejuni isolates from a significant Canadian water-borne outbreak were previously associated with the presence of a CJIE1 prophage. Whole genome sequencing was used to examine the genetic diversity among these isolates and confirm that previous observations could be attributed to differential prophage carriage. Moreover, we sought to determine the presence of genome sequences that could be used as surrogate markers to delineate outbreak-associated isolates. Differential carriage of the CJIE1 prophage was identified as the major genetic difference among the four outbreak isolates. High quality single-nucleotide variant (hqSNV) and core genome multilocus sequence typing (cgMLST) clustered these isolates within expanded datasets consisting of additional C. jejuni strains. The number and location of homopolymeric tract regions was identical in all four outbreak isolates but differed from all other C. jejuni examined. Comparative genomics and PCR amplification enabled the identification of large chromosomal inversions of approximately 93 kb and 388 kb within the outbreak isolates associated with transducer-like proteins containing long nucleotide repeat sequences. The 93-kb inversion was characteristic of the outbreak-associated isolates, and the gene content of this inverted region displayed high synteny with the reference strain. The four outbreak isolates were clonally derived and differed mainly in the presence of the CJIE1 prophage, validating earlier findings linking the prophage to phenotypic differences in virulence assays and protein expression. The identification of large, genetically syntenous chromosomal inversions in the genomes of outbreak-associated isolates provided a unique method for discriminating outbreak isolates from the background population. Transducer-like proteins appear to be associated with the chromosomal inversions. CgMLST and hqSNV analysis also effectively delineated the outbreak isolates within the larger C. jejuni population structure.

The structure-activity relationship of inhibitors of serotonin uptake and receptor binding

NASA Astrophysics Data System (ADS)

Hansch, Corwin; Caldwell, Jonathan

1991-10-01

An analysis of five different datasets of inhibitors of serotonin uptake has yielded quantitative structure/ activity relationships (QSARs) which delineate the role of steric and hydrophobic properties essential for inhibition by phenylethylamine-type analogues.

Infiltration and hydraulic connections from the Niagara River to a fractured-dolomite aquifer in Niagara Falls, New York

USGS Publications Warehouse

Yager, R.M.; Kappel, W.M.

1998-01-01

The spatial distribution of hydrogen and oxygen stable-isotope values in groundwater can be used to distinguish different sources of recharge and to trace groundwater flow directions from recharge boundaries. This method can be particularly useful in fractured-rock settings where multiple lines of evidence are required to delineate preferential flow paths that result from heterogeneity within fracture zones. Flow paths delineated with stable isotopes can be combined with hydraulic data to form a more complete picture of the groundwater flow system. In this study values of ??D and ??18O were used to delineate paths of river-water infiltration into the Lockport Group, a fractured dolomite aquifer, and to compute the percentage of fiver water in groundwater samples from shallow bedrock wells. Flow paths were correlated with areas of high hydraulic diffusivity in the shallow bedrock that were delineated from water-level fluctuations induced by diurnal stage fluctuations in man-made hydraulic structures. Flow paths delineated with the stable-isotope and hydraulic data suggest that fiver infiltration reaches an unlined storm sewer in the bedrock through a drainage system that surrounds aqueducts carrying river water to hydroelectric power plants. This finding is significant because the storm sewer is the discharge point for contaminated groundwater from several chemical waste-disposal sites and the cost of treating the storm sewer's discharge could be reduced if the volume of infiltration from the river were decreased.The spatial distribution of hydrogen and oxygen stable-isotope values in groundwater can be used to distinguish different sources of recharge and to trace groundwater flow directions from recharge boundaries. This method can be particularly useful in fractured-rock settings where multiple lines of evidence are required to delineate preferential flow paths that result from heterogeneity within fracture zones. Flow paths delineated with stable isotopes can be combined with hydraulic data to form a more complete picture of the groundwater flow system. In this study values of ??D and ??18O were used to delineate paths of river-water infiltration into the Lockport Group, a fractured dolomite aquifer, and to compute the percentage of river water in groundwater samples from shallow bedrock wells. Flow paths were correlated with areas of high hydraulic diffusivity in the shallow bedrock that were delineated from water-level fluctuations induced by diurnal stage fluctuations in man-made hydraulic structures. Flow paths delineated with the stable-isotope and hydraulic data suggest that river infiltration reaches an unlined storm sewer in the bedrock through a drainage system that surrounds aqueducts carrying river water to hydroelectric power plants. This finding is significant because the storm sewer is the discharge point for contaminated groundwater from several chemical waste-disposal sites and the cost of treating the storm sewer's discharge could be reduced if the volume of infiltration from the river were decreased.

Spatial structure increases the waiting time for cancer

PubMed Central

Martens, Erik A.; Kostadinov, Rumen; Maley, Carlo C.; Hallatschek, Oskar

2012-01-01

Cancer results from a sequence of genetic and epigenetic changes which lead to a variety of abnormal phenotypes including increased proliferation and survival of somatic cells, and thus, to a selective advantage of pre-cancerous cells. The notion of cancer progression as an evolutionary process has been experiencing increasing interest in recent years. Many efforts have been made to better understand and predict the progression to cancer using mathematical models; these mostly consider the evolution of a well-mixed cell population, even though pre-cancerous cells often evolve in highly structured epithelial tissues. In this study, we propose a novel model of cancer progression that considers a spatially structured cell population where clones expand via adaptive waves. This model is used to assess two different paradigms of asexual evolution that have been suggested to delineate the process of cancer progression. The standard scenario of periodic selection assumes that driver mutations are accumulated strictly sequentially over time. However, when the mutation supply is sufficiently high, clones may arise simultaneously on distinct genetic backgrounds, and clonal adaptation waves interfere with each other. We find that in the presence of clonal interference, spatial structure increases the waiting time for cancer, leads to a patchwork structure of non-uniformly sized clones, decreases the survival probability of virtually neutral (passenger) mutations, and that genetic distance begins to increase over a characteristic length scale Lc. These characteristic features of clonal interference may help to predict the onset of cancers with pronounced spatial structure and to interpret spatially-sampled genetic data obtained from biopsies. Our estimates suggest that clonal interference likely occurs in the progression of colon cancer, and possibly other cancers where spatial structure matters. PMID:22707911

Spatial structure increases the waiting time for cancer

NASA Astrophysics Data System (ADS)

Martens, Erik A.; Kostadinov, Rumen; Maley, Carlo C.; Hallatschek, Oskar

2011-11-01

Cancer results from a sequence of genetic and epigenetic changes that lead to a variety of abnormal phenotypes including increased proliferation and survival of somatic cells and thus to a selective advantage of pre-cancerous cells. The notion of cancer progression as an evolutionary process has been attracting increasing interest in recent years. A great deal of effort has been made to better understand and predict the progression to cancer using mathematical models; these mostly consider the evolution of a well-mixed cell population, even though pre-cancerous cells often evolve in highly structured epithelial tissues. In this study, we propose a novel model of cancer progression that considers a spatially structured cell population where clones expand via adaptive waves. This model is used to assess two different paradigms of asexual evolution that have been suggested to delineate the process of cancer progression. The standard scenario of periodic selection assumes that driver mutations are accumulated strictly sequentially over time. However, when the mutation supply is sufficiently high, clones may arise simultaneously on distinct genetic backgrounds, and clonal adaptation waves interfere with each other. We find that in the presence of clonal interference, spatial structure increases the waiting time for cancer, leads to a patchwork structure of non-uniformly sized clones and decreases the survival probability of virtually neutral (passenger) mutations, and that genetic distance begins to increase over a characteristic length scale Lc. These characteristic features of clonal interference may help us to predict the onset of cancers with pronounced spatial structure and to interpret spatially sampled genetic data obtained from biopsies. Our estimates suggest that clonal interference likely occurs in the progression of colon cancer and possibly other cancers where spatial structure matters.

Variation of normal tissue complication probability (NTCP) estimates of radiation-induced hypothyroidism in relation to changes in delineation of the thyroid gland.

PubMed

Rønjom, Marianne F; Brink, Carsten; Lorenzen, Ebbe L; Hegedüs, Laszlo; Johansen, Jørgen

2015-01-01

To examine the variations of risk-estimates of radiation-induced hypothyroidism (HT) from our previously developed normal tissue complication probability (NTCP) model in patients with head and neck squamous cell carcinoma (HNSCC) in relation to variability of delineation of the thyroid gland. In a previous study for development of an NTCP model for HT, the thyroid gland was delineated in 246 treatment plans of patients with HNSCC. Fifty of these plans were randomly chosen for re-delineation for a study of the intra- and inter-observer variability of thyroid volume, Dmean and estimated risk of HT. Bland-Altman plots were used for assessment of the systematic (mean) and random [standard deviation (SD)] variability of the three parameters, and a method for displaying the spatial variation in delineation differences was developed. Intra-observer variability resulted in a mean difference in thyroid volume and Dmean of 0.4 cm(3) (SD ± 1.6) and -0.5 Gy (SD ± 1.0), respectively, and 0.3 cm(3) (SD ± 1.8) and 0.0 Gy (SD ± 1.3) for inter-observer variability. The corresponding mean differences of NTCP values for radiation-induced HT due to intra- and inter-observer variations were insignificantly small, -0.4% (SD ± 6.0) and -0.7% (SD ± 4.8), respectively, but as the SDs show, for some patients the difference in estimated NTCP was large. For the entire study population, the variation in predicted risk of radiation-induced HT in head and neck cancer was small and our NTCP model was robust against observer variations in delineation of the thyroid gland. However, for the individual patient, there may be large differences in estimated risk which calls for precise delineation of the thyroid gland to obtain correct dose and NTCP estimates for optimized treatment planning in the individual patient.

10 CFR 54.21 - Contents of application-technical information.

Code of Federal Regulations, 2013 CFR

2013-01-01

... delineated in § 54.4, identify and list those structures and components subject to an aging management review. Structures and components subject to an aging management review shall encompass those structures and... component identified in paragraph (a)(1) of this section, demonstrate that the effects of aging will be...

10 CFR 54.21 - Contents of application-technical information.

Code of Federal Regulations, 2012 CFR

2012-01-01

... delineated in § 54.4, identify and list those structures and components subject to an aging management review. Structures and components subject to an aging management review shall encompass those structures and... component identified in paragraph (a)(1) of this section, demonstrate that the effects of aging will be...

10 CFR 54.21 - Contents of application-technical information.

Code of Federal Regulations, 2014 CFR

2014-01-01

... delineated in § 54.4, identify and list those structures and components subject to an aging management review. Structures and components subject to an aging management review shall encompass those structures and... component identified in paragraph (a)(1) of this section, demonstrate that the effects of aging will be...

Estimating the influence of population density and dispersal behavior on the ability to detect and monitor Agrilus planipennis (Coleoptera: Buprestidae) populations.

PubMed

Mercader, R J; Siegert, N W; McCullough, D G

2012-02-01

Emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), a phloem-feeding pest of ash (Fraxinus spp.) trees native to Asia, was first discovered in North America in 2002. Since then, A. planipennis has been found in 15 states and two Canadian provinces and has killed tens of millions of ash trees. Understanding the probability of detecting and accurately delineating low density populations of A. planipennis is a key component of effective management strategies. Here we approach this issue by 1) quantifying the efficiency of sampling nongirdled ash trees to detect new infestations of A. planipennis under varying population densities and 2) evaluating the likelihood of accurately determining the localized spread of discrete A. planipennis infestations. To estimate the probability a sampled tree would be detected as infested across a gradient of A. planipennis densities, we used A. planipennis larval density estimates collected during intensive surveys conducted in three recently infested sites with known origins. Results indicated the probability of detecting low density populations by sampling nongirdled trees was very low, even when detection tools were assumed to have three-fold higher detection probabilities than nongirdled trees. Using these results and an A. planipennis spread model, we explored the expected accuracy with which the spatial extent of an A. planipennis population could be determined. Model simulations indicated a poor ability to delineate the extent of the distribution of localized A. planipennis populations, particularly when a small proportion of the population was assumed to have a higher propensity for dispersal.

Existential loneliness: a review of the concept, its psychosocial precipitants and psychotherapeutic implications for HIV-infected women.

PubMed

Mayers, A M; Svartberg, M

2001-12-01

While many of the most urgent psychological issues that women with HIV infection are forced to deal with are existential in nature, and therefore shared by humankind, the assault on their physical and psychological being may trigger these issues in large amounts and may overwhelm their defensive structure. Existential loneliness may be an important existential issue that arises for these women and needs to be given equal consideration alongside other forms of loneliness. In this paper, we review the concept of existential loneliness, discuss methods to measure the concept, delineate some central psychosocial precipitants of existential loneliness among HIV-infected women, and discuss psychotherapeutic implications. We conclude that incorporating an existential philosophical perspective into one's particular therapeutic stance may be especially appropriate with this population.

Enhancing in vivo tumor boundary delineation with structured illumination fluorescence molecular imaging and spatial gradient mapping

NASA Astrophysics Data System (ADS)

Sun, Jessica; Miller, Jessica P.; Hathi, Deep; Zhou, Haiying; Achilefu, Samuel; Shokeen, Monica; Akers, Walter J.

2016-08-01

Fluorescence imaging, in combination with tumor-avid near-infrared (NIR) fluorescent molecular probes, provides high specificity and sensitivity for cancer detection in preclinical animal models, and more recently, assistance during oncologic surgery. However, conventional camera-based fluorescence imaging techniques are heavily surface-weighted such that surface reflection from skin or other nontumor tissue and nonspecific fluorescence signals dominate, obscuring true cancer-specific signals and blurring tumor boundaries. To address this challenge, we applied structured illumination fluorescence molecular imaging (SIFMI) in live animals for automated subtraction of nonspecific surface signals to better delineate accumulation of an NIR fluorescent probe targeting α4β1 integrin in mice bearing subcutaneous plasma cell xenografts. SIFMI demonstrated a fivefold improvement in tumor-to-background contrast when compared with other full-field fluorescence imaging methods and required significantly reduced scanning time compared with diffuse optical spectroscopy imaging. Furthermore, the spatial gradient mapping enhanced highlighting of tumor boundaries. Through the relatively simple hardware and software modifications described, SIFMI can be integrated with clinical fluorescence imaging systems, enhancing intraoperative tumor boundary delineation from the uninvolved tissue.

Application of the Geo-Anomaly Unit Concept in Quantitative Delineation and Assessment of Gold Ore Targets in Western Shandong Uplift Terrain, Eastern China

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chen Yongqing, E-mail: ydonglai@mail.cgs.gov.cn; Zhao Pengda; Chen Jianguo

2001-03-15

A number of large and giant ore deposits have been discovered within the relatively small areas of lithospheric structure anomalies, including various boundary zones of tectonic plates. The regions have become the well-known intercontinental ore-forming belts, such as the circum-Pacific gold-copper, copper-molybdenum, and tungsten-tin metallogenic belts. These belts are typical geological anomalous areas. An investigation into the hydrothermal ore deposits in different regions in the former Soviet Union illustrated that the geologic structures of ore fields of almost all major commercial deposits have distinct features compared with the neighboring areas. These areas with distinct features are defined as geo-anomalies. Amore » geo-anomaly refers to such a geologic body or a combination of bodies that their composition, texture-structure, and genesis are significantly different from those of their surroundings. A geo-anomaly unit (GU) is an area containing distinct features that can be delineated with integrated ore-forming information using computer techniques on the basis of the geo-anomaly concept. Herein, the GU concept is illustrated by a case study of delineating the gold ore targets in the western Shandong uplift terrain, eastern China. It includes: (1) analyses of gold ore-forming factors; (2) compilation of normalized regional geochemical map and extraction of geochemical anomalies; (3) compilation of gravitational and aeromagnetic tectonic skeleton map and extraction of gravitational and aeromagnetic anomalies; (4) extraction of circular and linear anomalies from remote-sensing Landsat TM images; (5) establishment of a geo-anomaly conceptual model associated with known gold mineralization; (6) establishment of gold ore-forming favorability by computing techniques; and (7) delineation and assessment of ore-forming units. The units with high favorability are suggested as ore targets.« less

Extensive sampling of polar bears (Ursus maritimus) in the Northwest Passage (Canadian Arctic Archipelago) reveals population differentiation across multiple spatial and temporal scales.

PubMed

Campagna, Leonardo; Van Coeverden de Groot, Peter J; Saunders, Brenda L; Atkinson, Stephen N; Weber, Diana S; Dyck, Markus G; Boag, Peter T; Lougheed, Stephen C

2013-09-01

As global warming accelerates the melting of Arctic sea ice, polar bears (Ursus maritimus) must adapt to a rapidly changing landscape. This process will necessarily alter the species distribution together with population dynamics and structure. Detailed knowledge of these changes is crucial to delineating conservation priorities. Here, we sampled 361 polar bears from across the center of the Canadian Arctic Archipelago spanning the Gulf of Boothia (GB) and M'Clintock Channel (MC). We use DNA microsatellites and mitochondrial control region sequences to quantify genetic differentiation, estimate gene flow, and infer population history. Two populations, roughly coincident with GB and MC, are significantly differentiated at both nuclear (F ST = 0.01) and mitochondrial (ΦST = 0.47; F ST = 0.29) loci, allowing Bayesian clustering analyses to assign individuals to either group. Our data imply that the causes of the mitochondrial and nuclear genetic patterns differ. Analysis of mtDNA reveals the matrilineal structure dates at least to the Holocene, and is common to individuals throughout the species' range. These mtDNA differences probably reflect both genetic drift and historical colonization dynamics. In contrast, the differentiation inferred from microsatellites is only on the scale of hundreds of years, possibly reflecting contemporary impediments to gene flow. Taken together, our data suggest that gene flow is insufficient to homogenize the GB and MC populations and support the designation of GB and MC as separate polar bear conservation units. Our study also provide a striking example of how nuclear DNA and mtDNA capture different aspects of a species demographic history.

Genotype-Phenotype Study of the Middle Gangetic Plain in India Shows Association of rs2470102 with Skin Pigmentation.

PubMed

Mishra, Anshuman; Nizammuddin, Sheikh; Mallick, Chandana Basu; Singh, Sakshi; Prakash, Satya; Siddiqui, Niyamat Ali; Rai, Niraj; Carlus, S Justin; Sudhakar, Digumarthi V S; Tripathi, Vishnu P; Möls, Märt; Kim-Howard, Xana; Dewangan, Hemlata; Mishra, Abhishek; Reddy, Alla G; Roy, Biswajit; Pandey, Krishna; Chaubey, Gyaneshwer; Das, Pradeep; Nath, Swapan K; Singh, Lalji; Thangaraj, Kumarasamy

2017-03-01

Our understanding of the genetics of skin pigmentation has been largely skewed towards populations of European ancestry, imparting less attention to South Asian populations, who behold huge pigmentation diversity. Here, we investigate skin pigmentation variation in a cohort of 1,167 individuals in the Middle Gangetic Plain of the Indian subcontinent. Our data confirm the association of rs1426654 with skin pigmentation among South Asians, consistent with previous studies, and also show association for rs2470102 single nucleotide polymorphism. Our haplotype analyses further help us delineate the haplotype distribution across social categories and skin color. Taken together, our findings suggest that the social structure defined by the caste system in India has a profound influence on the skin pigmentation patterns of the subcontinent. In particular, social category and associated single nucleotide polymorphisms explain about 32% and 6.4%, respectively, of the total phenotypic variance. Phylogeography of the associated single nucleotide polymorphisms studied across 52 diverse populations of the Indian subcontinent shows wide presence of the derived alleles, although their frequencies vary across populations. Our results show that both polymorphisms (rs1426654 and rs2470102) play an important role in the skin pigmentation diversity of South Asians. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

Distribution and effects of acidic deposition on wildlife and ecosystems

USGS Publications Warehouse

Stromborg, K.L.; Longcore, J.R.; Kaemar, Peter; Legath, J.

1987-01-01

Acidic deposition occurs over most of the United States and the deposition patterns and theoretical vulnerabilities of aquatic ecosystems to chemical changes can be delineated, but few data exist on concomitant biological effects. Hypothetical direct effects are limited primarily to toxicity of various heavy metals mobilized at reduced pH. Results of studies in Scandinavia suggest that aluminum interferes with avian reproduction near acidified lakes. Some amphibian populations located on acid-vulnerable substrates may be adversely affected by reduced pH in the vernal pools used for egg laying and larval growth. Indirect effects on populations are difficult to detect because few historical data exist for wildlife populations and trophic relationships in vulnerable areas. Current research in the U.S.A. focuses on measuring habitat characteristics, food availability, and avian use of vulnerable wetland habitats. Results of Scandinavian studies suggest that some species of waterfowl may prefer acidified, I fish-free habitats because invertebrates essential for meeting nutritional requirements are more easily obtained in the absence of competition from fish. However, avian species dependent on fish would be absent from these habitats. Alteration of either the vegetative structure or primary productivity of wetlands might indirectly affect avian populations by causing decreased invertebrate productivity and consequent food limitations for birds.

Geological and hydrogeological investigation in West Malaysia

NASA Technical Reports Server (NTRS)

Ahmad, J. B. (Principal Investigator)

1976-01-01

The author has identified the following significant results. The broad synoptic view of the images allowed easy identification of circular features and major fault traces in low lying areas. Sedimentary units were delineated in accordance with the prevailing rock types and where applicable the folding characteristics. Igneous units could easily be differentiated by tone, degree of fracturing, texture, and drainage pattern. The larger fold structures, anticlinoriums and synclinoriums, of the younger sediments on the eastern edge of the central belt could also be easily delineated.

Evaluation of vector coastline features extracted from 'structure from motion'-derived elevation data

USGS Publications Warehouse

Kinsman, Nicole; Gibbs, Ann E.; Nolan, Matt

2015-01-01

For extensive and remote coastlines, the absence of high-quality elevation models—for example, those produced with lidar—leaves some coastal populations lacking one of the essential elements for mapping shoreline positions or flood extents. Here, we compare seven different elevation products in a lowlying area in western Alaska to establish their appropriateness for coastal mapping applications that require the delineation of elevation-based vectors. We further investigate the effective use of a Structure from Motion (SfM)-derived surface model (vertical RMSE<20 cm) by generating a tidal datum-based shoreline and an inundation extent map for a 2011 flood event. Our results suggest that SfM-derived elevation products can yield elevation-based vector features that have horizontal positional uncertainties comparable to those derived from other techniques. We also provide a rule-of-thumb equation to aid in the selection of minimum elevation model specifications based on terrain slope, vertical uncertainties, and desired horizontal accuracy.

Watershed delineation and nitrogen source analysis for Bayou Chico, an urban watershed in northwest Florida

EPA Science Inventory

Nutrient pollution in stormwater runoff from urbanized areas contributes to water quality degradation in streams and receiving waterbodies. Agriculture, population growth, and industrial activities are significant sources of nitrogen inputs for surface waters. Increased nitrogen ...

Genetic structure of lake whitefish (Coregonus clupeaformis) in Lake Michigan

USGS Publications Warehouse

VanDeHey, J.A.; Sloss, Brian L.; Peeters, Paul J.; Sutton, T.M.

2009-01-01

Genetic relationships among lake whitefish (Coregonus clupeaformis) spawning aggregates in Lake Michigan were assessed and used to predict a stock or management unit (MU) model for the resource. We hypothesized that distinct spawning aggregates represented potential MUs and that differences at molecular markers underlie population differentiation. Genetic stock identification using 11 microsatellite loci indicated the presence of six genetic MUs. Resolved MUs corresponded to geographically proximate spawning aggregates clustering into genetic groups. Within MUs, analyses suggested that all but one delineated MU was a stable grouping (i.e., no between-population differences), with the exception being the Hog Island - Traverse Bay grouping. Elk Rapids was the most genetically divergent population within Lake Michigan. However, low F st values suggested that moderate to high levels of gene flow occur or have occurred in the past between MUs. Significant tests of isolation by distance and low pairwise Fst values potentially led to conflicting results between traditional analyses and a Bayesian approach. This data set could provide baseline data from which a comprehensive mixed-stock analysis could be performed, allowing for more efficient and effective management of this economically and socially important resource.

A nuclear DNA perspective on delineating evolutionarily significant lineages in polyploids: the case of the endangered shortnose sturgeon (Acipenser brevirostrum)

USGS Publications Warehouse

King, Timothy L.; Henderson, Anne P.; Kynard, Boyd E.; Kieffer, Micah C.; Peterson, Douglas L.; Aunins, Aaron W.; Brown, Bonnie L.

2014-01-01

The shortnose sturgeon, Acipenser brevirostrum, oft considered a phylogenetic relic, is listed as an “endangered species threatened with extinction” in the US and “Vulnerable” on the IUCN Red List. Effective conservation of A. brevirostrum depends on understanding its diversity and evolutionary processes, yet challenges associated with the polyploid nature of its nuclear genome have heretofore limited population genetic analysis to maternally inherited haploid characters. We developed a suite of polysomic microsatellite DNA markers and characterized a sample of 561 shortnose sturgeon collected from major extant populations along the North American Atlantic coast. The 181 alleles observed at 11 loci were scored as binary loci and the data were subjected to multivariate ordination, Bayesian clustering, hierarchical partitioning of variance, and among-population distance metric tests. The methods uncovered moderately high levels of gene diversity suggesting population structuring across and within three metapopulations (Northeast, Mid-Atlantic, and Southeast) that encompass seven demographically discrete and evolutionarily distinct lineages. The predicted groups are consistent with previously described behavioral patterns, especially dispersal and migration, supporting the interpretation that A. brevirostrum exhibit adaptive differences based on watershed. Combined with results of prior genetic (mitochondrial DNA) and behavioral studies, the current work suggests that dispersal is an important factor in maintaining genetic diversity in A. brevirostrum and that the basic unit for conservation management is arguably the local population.

A nuclear DNA perspective on delineating evolutionarily significant lineages in polyploids: the case of the endangered shortnose sturgeon (Acipenser brevirostrum).

PubMed

King, Tim L; Henderson, Anne P; Kynard, Boyd E; Kieffer, Micah C; Peterson, Douglas L; Aunins, Aaron W; Brown, Bonnie L

2014-01-01

The shortnose sturgeon, Acipenser brevirostrum, oft considered a phylogenetic relic, is listed as an "endangered species threatened with extinction" in the US and "Vulnerable" on the IUCN Red List. Effective conservation of A. brevirostrum depends on understanding its diversity and evolutionary processes, yet challenges associated with the polyploid nature of its nuclear genome have heretofore limited population genetic analysis to maternally inherited haploid characters. We developed a suite of polysomic microsatellite DNA markers and characterized a sample of 561 shortnose sturgeon collected from major extant populations along the North American Atlantic coast. The 181 alleles observed at 11 loci were scored as binary loci and the data were subjected to multivariate ordination, Bayesian clustering, hierarchical partitioning of variance, and among-population distance metric tests. The methods uncovered moderately high levels of gene diversity suggesting population structuring across and within three metapopulations (Northeast, Mid-Atlantic, and Southeast) that encompass seven demographically discrete and evolutionarily distinct lineages. The predicted groups are consistent with previously described behavioral patterns, especially dispersal and migration, supporting the interpretation that A. brevirostrum exhibit adaptive differences based on watershed. Combined with results of prior genetic (mitochondrial DNA) and behavioral studies, the current work suggests that dispersal is an important factor in maintaining genetic diversity in A. brevirostrum and that the basic unit for conservation management is arguably the local population.

Even in Public Television, Ownership Changes Matter.

ERIC Educational Resources Information Center

Lashley, Marilyn E.

1992-01-01

Shows that executive turnover through four administrations (1967-89) has kept public broadcasting mired in budgetary crises, transforming its goals, structure, and programing content over time. Describes policy preferences imposed by administrators, identifies attendant modifications in organizational structure, and delineates effects of these…

A wavelet-based ECG delineation algorithm for 32-bit integer online processing

PubMed Central

2011-01-01

Background Since the first well-known electrocardiogram (ECG) delineator based on Wavelet Transform (WT) presented by Li et al. in 1995, a significant research effort has been devoted to the exploitation of this promising method. Its ability to reliably delineate the major waveform components (mono- or bi-phasic P wave, QRS, and mono- or bi-phasic T wave) would make it a suitable candidate for efficient online processing of ambulatory ECG signals. Unfortunately, previous implementations of this method adopt non-linear operators such as root mean square (RMS) or floating point algebra, which are computationally demanding. Methods This paper presents a 32-bit integer, linear algebra advanced approach to online QRS detection and P-QRS-T waves delineation of a single lead ECG signal, based on WT. Results The QRS detector performance was validated on the MIT-BIH Arrhythmia Database (sensitivity Se = 99.77%, positive predictive value P+ = 99.86%, on 109010 annotated beats) and on the European ST-T Database (Se = 99.81%, P+ = 99.56%, on 788050 annotated beats). The ECG delineator was validated on the QT Database, showing a mean error between manual and automatic annotation below 1.5 samples for all fiducial points: P-onset, P-peak, P-offset, QRS-onset, QRS-offset, T-peak, T-offset, and a mean standard deviation comparable to other established methods. Conclusions The proposed algorithm exhibits reliable QRS detection as well as accurate ECG delineation, in spite of a simple structure built on integer linear algebra. PMID:21457580

A wavelet-based ECG delineation algorithm for 32-bit integer online processing.

PubMed

Di Marco, Luigi Y; Chiari, Lorenzo

2011-04-03

Since the first well-known electrocardiogram (ECG) delineator based on Wavelet Transform (WT) presented by Li et al. in 1995, a significant research effort has been devoted to the exploitation of this promising method. Its ability to reliably delineate the major waveform components (mono- or bi-phasic P wave, QRS, and mono- or bi-phasic T wave) would make it a suitable candidate for efficient online processing of ambulatory ECG signals. Unfortunately, previous implementations of this method adopt non-linear operators such as root mean square (RMS) or floating point algebra, which are computationally demanding. This paper presents a 32-bit integer, linear algebra advanced approach to online QRS detection and P-QRS-T waves delineation of a single lead ECG signal, based on WT. The QRS detector performance was validated on the MIT-BIH Arrhythmia Database (sensitivity Se = 99.77%, positive predictive value P+ = 99.86%, on 109010 annotated beats) and on the European ST-T Database (Se = 99.81%, P+ = 99.56%, on 788050 annotated beats). The ECG delineator was validated on the QT Database, showing a mean error between manual and automatic annotation below 1.5 samples for all fiducial points: P-onset, P-peak, P-offset, QRS-onset, QRS-offset, T-peak, T-offset, and a mean standard deviation comparable to other established methods. The proposed algorithm exhibits reliable QRS detection as well as accurate ECG delineation, in spite of a simple structure built on integer linear algebra.

Delineating wetland catchments and modeling hydrologic connectivity using lidar data and aerial imagery

NASA Astrophysics Data System (ADS)

Wu, Qiusheng; Lane, Charles R.

2017-07-01

In traditional watershed delineation and topographic modeling, surface depressions are generally treated as spurious features and simply removed from a digital elevation model (DEM) to enforce flow continuity of water across the topographic surface to the watershed outlets. In reality, however, many depressions in the DEM are actual wetland landscape features with seasonal to permanent inundation patterning characterized by nested hierarchical structures and dynamic filling-spilling-merging surface-water hydrological processes. Differentiating and appropriately processing such ecohydrologically meaningful features remains a major technical terrain-processing challenge, particularly as high-resolution spatial data are increasingly used to support modeling and geographic analysis needs. The objectives of this study were to delineate hierarchical wetland catchments and model their hydrologic connectivity using high-resolution lidar data and aerial imagery. The graph-theory-based contour tree method was used to delineate the hierarchical wetland catchments and characterize their geometric and topological properties. Potential hydrologic connectivity between wetlands and streams were simulated using the least-cost-path algorithm. The resulting flow network delineated potential flow paths connecting wetland depressions to each other or to the river network on scales finer than those available through the National Hydrography Dataset. The results demonstrated that our proposed framework is promising for improving overland flow simulation and hydrologic connectivity analysis.

Labeling for Big Data in radiation oncology: The Radiation Oncology Structures ontology.

PubMed

Bibault, Jean-Emmanuel; Zapletal, Eric; Rance, Bastien; Giraud, Philippe; Burgun, Anita

2018-01-01

Leveraging Electronic Health Records (EHR) and Oncology Information Systems (OIS) has great potential to generate hypotheses for cancer treatment, since they directly provide medical data on a large scale. In order to gather a significant amount of patients with a high level of clinical details, multicenter studies are necessary. A challenge in creating high quality Big Data studies involving several treatment centers is the lack of semantic interoperability between data sources. We present the ontology we developed to address this issue. Radiation Oncology anatomical and target volumes were categorized in anatomical and treatment planning classes. International delineation guidelines specific to radiation oncology were used for lymph nodes areas and target volumes. Hierarchical classes were created to generate The Radiation Oncology Structures (ROS) Ontology. The ROS was then applied to the data from our institution. Four hundred and seventeen classes were created with a maximum of 14 children classes (average = 5). The ontology was then converted into a Web Ontology Language (.owl) format and made available online on Bioportal and GitHub under an Apache 2.0 License. We extracted all structures delineated in our department since the opening in 2001. 20,758 structures were exported from our "record-and-verify" system, demonstrating a significant heterogeneity within a single center. All structures were matched to the ROS ontology before integration into our clinical data warehouse (CDW). In this study we describe a new ontology, specific to radiation oncology, that reports all anatomical and treatment planning structures that can be delineated. This ontology will be used to integrate dosimetric data in the Assistance Publique-Hôpitaux de Paris CDW that stores data from 6.5 million patients (as of February 2017).

Labeling for Big Data in radiation oncology: The Radiation Oncology Structures ontology

PubMed Central

Zapletal, Eric; Rance, Bastien; Giraud, Philippe; Burgun, Anita

2018-01-01

Purpose Leveraging Electronic Health Records (EHR) and Oncology Information Systems (OIS) has great potential to generate hypotheses for cancer treatment, since they directly provide medical data on a large scale. In order to gather a significant amount of patients with a high level of clinical details, multicenter studies are necessary. A challenge in creating high quality Big Data studies involving several treatment centers is the lack of semantic interoperability between data sources. We present the ontology we developed to address this issue. Methods Radiation Oncology anatomical and target volumes were categorized in anatomical and treatment planning classes. International delineation guidelines specific to radiation oncology were used for lymph nodes areas and target volumes. Hierarchical classes were created to generate The Radiation Oncology Structures (ROS) Ontology. The ROS was then applied to the data from our institution. Results Four hundred and seventeen classes were created with a maximum of 14 children classes (average = 5). The ontology was then converted into a Web Ontology Language (.owl) format and made available online on Bioportal and GitHub under an Apache 2.0 License. We extracted all structures delineated in our department since the opening in 2001. 20,758 structures were exported from our “record-and-verify” system, demonstrating a significant heterogeneity within a single center. All structures were matched to the ROS ontology before integration into our clinical data warehouse (CDW). Conclusion In this study we describe a new ontology, specific to radiation oncology, that reports all anatomical and treatment planning structures that can be delineated. This ontology will be used to integrate dosimetric data in the Assistance Publique—Hôpitaux de Paris CDW that stores data from 6.5 million patients (as of February 2017). PMID:29351341

Function in the Human Connectome: Task-fMRI and Individual Differences in Behavior

PubMed Central

Barch, Deanna M.; Burgess, Gregory C.; Harms, Michael P.; Petersen, Steven E.; Schlaggar, Bradley L.; Corbetta, Maurizio; Glasser, Matthew F.; Curtiss, Sandra; Dixit, Sachin; Feldt, Cindy; Nolan, Dan; Bryant, Edward; Hartley, Tucker; Footer, Owen; Bjork, James M.; Poldrack, Russ; Smith, Steve; Johansen-Berg, Heidi; Snyder, Abraham Z.; Van Essen, David C.

2014-01-01

The primary goal of the Human Connectome Project (HCP) is to delineate the typical patterns of structural and functional connectivity in the healthy adult human brain. However, we know that there are important individual differences in such patterns of connectivity, with evidence that this variability is associated with alterations in important cognitive and behavioral variables that affect real world function. The HCP data will be a critical stepping-off point for future studies that will examine how variation in human structural and functional connectivity play a role in adult and pediatric neurological and psychiatric disorders that account for a huge amount of public health resources. Thus, the HCP is collecting behavioral measures of a range of motor, sensory, cognitive and emotional processes that will delineate a core set of functions relevant to understanding the relationship between brain connectivity and human behavior. In addition, the HCP is using task-fMRI (tfMRI) to help delineate the relationships between individual differences in the neurobiological substrates of mental processing and both functional and structural connectivity, as well as to help characterize and validate the connectivity analyses to be conducted on the structural and functional connectivity data. This paper describes the logic and rationale behind the development of the behavioral, individual difference, and tfMRI batteries and provides preliminary data on the patterns of activation associated with each of the fMRI tasks, at both a group and individual level. PMID:23684877

Combining watershed and graph cuts methods to segment organs at risk in radiotherapy

NASA Astrophysics Data System (ADS)

Dolz, Jose; Kirisli, Hortense A.; Viard, Romain; Massoptier, Laurent

2014-03-01

Computer-aided segmentation of anatomical structures in medical images is a valuable tool for efficient radiation therapy planning (RTP). As delineation errors highly affect the radiation oncology treatment, it is crucial to delineate geometric structures accurately. In this paper, a semi-automatic segmentation approach for computed tomography (CT) images, based on watershed and graph-cuts methods, is presented. The watershed pre-segmentation groups small areas of similar intensities in homogeneous labels, which are subsequently used as input for the graph-cuts algorithm. This methodology does not require of prior knowledge of the structure to be segmented; even so, it performs well with complex shapes and low intensity. The presented method also allows the user to add foreground and background strokes in any of the three standard orthogonal views - axial, sagittal or coronal - making the interaction with the algorithm easy and fast. Hence, the segmentation information is propagated within the whole volume, providing a spatially coherent result. The proposed algorithm has been evaluated using 9 CT volumes, by comparing its segmentation performance over several organs - lungs, liver, spleen, heart and aorta - to those of manual delineation from experts. A Dicés coefficient higher than 0.89 was achieved in every case. That demonstrates that the proposed approach works well for all the anatomical structures analyzed. Due to the quality of the results, the introduction of the proposed approach in the RTP process will be a helpful tool for organs at risk (OARs) segmentation.

Integrating gravity and magnetic field data to delineate structurally controlled gold mineralization in the Sefwi Belt of Ghana

NASA Astrophysics Data System (ADS)

Konadu Amoah, Bernard; Dadzie, Isaac; Takyi-Kyeremeh, Kwaku

2018-08-01

Gravity and magnetic surveys were used to delineate potential gold mineralization zones in the Sefwi belt of Ghana. The study area is an intrusive dominated area that hosts pockets of small scale mining operations locally referred to as Galamsey. These Galamsey operations are not guided by a scientific approach to back the trend of gold mineralization which is conventionally mined. The study aimed at mapping lithological units, structural setting and relating Galamsey sites to delineate potential zones of gold mineralization. A Scintrex CG5 gravimeter and GEM’s Overhauser magnetometer were used for gravity and magnetic data acquisition respectively. The magnetic data were corrected and enhancing filters such as reduction to the pole (RTP), analytical signal and first vertical derivative were applied using Oasis montaj 7.1. Gravity data were also reduced to the geoid using the Oasis montaj software to produce a complete Bouguer anomaly map. The regional/residual separation technique produced a residual gravity map. The RTP and analytical signal filters from the magnetic data and residual gravity anomaly map from the gravity data helped in mapping belt type (Dixcove) Birimian granitoids and mafic intrusive unit, interpreted as gabbro. The first vertical derivative filter was useful in mapping NE/SW minor faults and crosscutting dykes largely concentrated in the belt type Birimian granitoids. All the three mapped Galamsey sites fell on a minor fault and are associated with the belt type granitoids which were used in delineating four potential zones of gold mineralization.

Cryptocoryne beckettii complex (Araceae) introduced at a Florida spring

USGS Publications Warehouse

Jacono, C.C.

2002-01-01

A vegetative population of Cryptocoryne (Araceae), introduced at a Florida spring, appeared to represent three closely related species in the C. beckettii complex: C. beckettii Thw. ex Trimen, C. wendtii de Wit and C. undulata Wendt. Individuals of C. undulata were true to type and could be delineated at the site. Intergradation of diagnostic features was common in others, upon transplanting and flowering. While some transplants produced spathes characteristic of either C. wendtii or C. beckettii, intermediates between the two species were common. Neither C. beckettii nor C. wendtii could be delineated at the site. The seclusion of the stream and the integrity of native plant communities have likely prevented dispersal downstream.

Genetic diversity and structure of a rare endemic cactus and an assessment of its genetic relationship with a more common congener.

PubMed

Rayamajhi, Niraj; Sharma, Jyotsna

2018-06-01

Endemic, obligate outcrossing plant species with narrow geographic distributions and disjunct populations are prone to loss of genetic diversity. Simultaneously, delineating clear species boundaries is important for targeted conservation efforts. The rare and endemic cactus, Sclerocactus brevihamatus subsp. tobuschii (SBT), has a parapatric relationship with Sclerocactus brevihamatus subsp. brevihamatus (SBB) but genetic distance between the two taxa is unknown. We: (1) developed taxon-specific polymorphic microsatellites, (2) assessed genetic diversity within and among nine populations of SBT, and within one population of SBB, and (3) estimated the genetic relationship between the two subspecies. Within-population genetic diversity of SBT was moderate to high (mean H o  = 0.37; mean H e  = 0.59). Indirect estimate of inbreeding corrected for null alleles (F is-INEst ) was low for SBT, ranging from 0.03 to 0.14 (mean F is-INEst  = 0.07). Genetic differentiation among populations of SBT was low based on F st (0.08) and AMOVA (Ф PT  = 0.10). Lack of genetic and spatial correlation in SBT populations coupled with the presence of private alleles and bottleneck events in several populations suggests that reproductive isolation is occurring but that sufficient time may not have yet passed to manifest strong differentiation. Cluster analyses segregated the 10 populations into three distinct groups, and separated SBB genotypes clearly. Results suggest that while hybridization between the two subspecies may occur, SBT is clearly differentiated genetically from SBB to retain its current taxonomic status.

Recognition of upper airway and surrounding structures at MRI in pediatric PCOS and OSAS

NASA Astrophysics Data System (ADS)

Tong, Yubing; Udupa, J. K.; Odhner, D.; Sin, Sanghun; Arens, Raanan

2013-03-01

Obstructive Sleep Apnea Syndrome (OSAS) is common in obese children with risk being 4.5 fold compared to normal control subjects. Polycystic Ovary Syndrome (PCOS) has recently been shown to be associated with OSAS that may further lead to significant cardiovascular and neuro-cognitive deficits. We are investigating image-based biomarkers to understand the architectural and dynamic changes in the upper airway and the surrounding hard and soft tissue structures via MRI in obese teenage children to study OSAS. At the previous SPIE conferences, we presented methods underlying Fuzzy Object Models (FOMs) for Automatic Anatomy Recognition (AAR) based on CT images of the thorax and the abdomen. The purpose of this paper is to demonstrate that the AAR approach is applicable to a different body region and image modality combination, namely in the study of upper airway structures via MRI. FOMs were built hierarchically, the smaller sub-objects forming the offspring of larger parent objects. FOMs encode the uncertainty and variability present in the form and relationships among the objects over a study population. Totally 11 basic objects (17 including composite) were modeled. Automatic recognition for the best pose of FOMs in a given image was implemented by using four methods - a one-shot method that does not require search, another three searching methods that include Fisher Linear Discriminate (FLD), a b-scale energy optimization strategy, and optimum threshold recognition method. In all, 30 multi-fold cross validation experiments based on 15 patient MRI data sets were carried out to assess the accuracy of recognition. The results indicate that the objects can be recognized with an average location error of less than 5 mm or 2-3 voxels. Then the iterative relative fuzzy connectedness (IRFC) algorithm was adopted for delineation of the target organs based on the recognized results. The delineation results showed an overall FP and TP volume fraction of 0.02 and 0.93.

Reduction of observer variation using matched CT-PET for lung cancer delineation: A three-dimensional analysis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Steenbakkers, Roel; Duppen, Joop C.; Fitton, Isabelle

2006-02-01

Purpose: Target delineation using only CT information introduces large geometric uncertainties in radiotherapy for lung cancer. Therefore, a reduction of the delineation variability is needed. The impact of including a matched CT scan with 2-[{sup 18}F]fluoro-2-deoxy-D-glucose positron emission tomography (FDG-PET) and adaptation of the delineation protocol and software on target delineation in lung cancer was evaluated in an extensive multi-institutional setting and compared with the delineations using CT only. Methods and Materials: The study was separated into two phases. For the first phase, 11 radiation oncologists (observers) delineated the gross tumor volume (GTV), including the pathologic lymph nodes of 22more » lung cancer patients (Stages I-IIIB) on CT only. For the second phase (1 year later), the same radiation oncologists delineated the GTV of the same 22 patients on a matched CT-FDG-PET scan using an adapted delineation protocol and software (according to the results of the first phase). All delineated volumes were analyzed in detail. The observer variation was computed in three dimensions by measuring the distance between the median GTV surface and each individual GTV. The variation in distance of all radiation oncologists was expressed as a standard deviation. The observer variation was evaluated for anatomic regions (lung, mediastinum, chest wall, atelectasis, and lymph nodes) and interpretation regions (agreement and disagreement; i.e., >80% vs. <80% of the radiation oncologists delineated the same structure, respectively). All radiation oncologist-computer interactions were recorded and analyzed with a tool called 'Big Brother.' Results: The overall three-dimensional observer variation was reduced from 1.0 cm (SD) for the first phase (CT only) to 0.4 cm (SD) for the second phase (matched CT-FDG-PET). The largest reduction in the observer variation was seen in the atelectasis region (SD 1.9 cm reduced to 0.5 cm). The mean ratio between the common and encompassing volume was 0.17 and 0.29 for the first and second phases, respectively. For the first phase, the common volume was 0 in 4 patients (i.e., no common point for all GTVs). In the second phase, the common volume was always >0. For all anatomic regions, the interpretation differences among the radiation oncologists were reduced. The amount of disagreement was 45% and 18% for the first and second phase, respectively. Furthermore, the mean delineation time (12 vs. 16 min, p < 0.001) and mean number of corrections (25 vs. 39, p < 0.001) were reduced in the second phase compared with the first phase. Conclusion: For high-precision radiotherapy, the delineation of lung target volumes using only CT introduces too great a variability among radiation oncologists. Implementing matched CT-FDG-PET and adapted delineation protocol and software reduced observer variation in lung cancer delineation significantly with respect to CT only. However, the remaining observer variation was still large compared with other geometric uncertainties (setup variation and organ motion)« less

Breakpoint structure of the Anopheles gambiae 2Rb chromosomal inversion.

PubMed

Lobo, Neil F; Sangaré, Djibril M; Regier, Allison A; Reidenbach, Kyanne R; Bretz, David A; Sharakhova, Maria V; Emrich, Scott J; Traore, Sekou F; Costantini, Carlo; Besansky, Nora J; Collins, Frank H

2010-10-25

Alternative arrangements of chromosome 2 inversions in Anopheles gambiae are important sources of population structure, and are associated with adaptation to environmental heterogeneity. The forces responsible for their origin and maintenance are incompletely understood. Molecular characterization of inversion breakpoints provides insight into how they arose, and provides the basis for development of molecular karyotyping methods useful in future studies. Sequence comparison of regions near the cytological breakpoints of 2Rb allowed the molecular delineation of breakpoint boundaries. Comparisons were made between the standard 2R+b arrangement in the An. gambiae PEST reference genome and the inverted 2Rb arrangements in the An. gambiae M and S genome assemblies. Sequence differences between alternative 2Rb arrangements were exploited in the design of a PCR diagnostic assay, which was evaluated against the known chromosomal banding pattern of laboratory colonies and field-collected samples from Mali and Cameroon. The breakpoints of the 7.55 Mb 2Rb inversion are flanked by extensive runs of the same short (72 bp) tandemly organized sequence, which was likely responsible for chromosomal breakage and rearrangement. Application of the molecular diagnostic assay suggested that 2Rb has a single common origin in An. gambiae and its sibling species, Anopheles arabiensis, and also that the standard arrangement (2R+b) may have arisen twice through breakpoint reuse. The molecular diagnostic was reliable when applied to laboratory colonies, but its accuracy was lower in natural populations. The complex repetitive sequence flanking the 2Rb breakpoint region may be prone to structural and sequence-level instability. The 2Rb molecular diagnostic has immediate application in studies based on laboratory colonies, but its usefulness in natural populations awaits development of complementary molecular tools.

Spatial and Temporal Variability of Elemental Signautres in Juvenile Winter Flounder (Psuedopleuronectes americanus): Implications for Natal Connectivity

EPA Science Inventory

Elemental signatures in otoliths (fish ear-stones) have become a powerful tool in fisheries science for identifying fish migration patterns, reconstructing environmental histories, and for delineating the nursery origins of adult fish populations. Assessing connectivity between a...

A Cognitive Perspective in the Treatment of Incarcerated Clients.

ERIC Educational Resources Information Center

Walsh, Thomas C.

1990-01-01

Proposes a cognitive therapy model as a workable approach in treating incarcerated clients. Reviews principal components and techniques of cognitive theory. Uses case vignettes to illustrate application of this approach. Delineates key features of cognitive model which relate to treatment of incarcerated population. (Author/ABL)

ELEMENTAL FINGERPRINTS OF JUVENILE WINTER FLOUNDER OTOLITHS FROM NARRAGNSETT BAY, RI AND SURROUNDING COASTAL PONDS

EPA Science Inventory

Elemental fingerprinting has become a powerful tool in fisheries science for identifying fish migration patterns, seasonal changes in habitat use, and for delineating the nursery origins of adult fish populations. In this study we investigated whether elemental signatures in juve...

10 CFR Appendix C to Part 73 - Nuclear Power Plant Safeguards Contingency Plans

Code of Federal Regulations, 2010 CFR

2010-01-01

.... Licensee's Organizational Structure for Contingency Responses—A delineation of the organization's chain of.... The following topics must be addressed: a. Organizational Structure. The safeguards contingency plan... individual, group, or organizational entity responsible for each decision and action. The goals of licensee...

STRUCTURAL AND HYDROGEOLOGIC APPLICATIONS OF REMOTE SENSING DATA, EASTERN YUCATAN PENINSULA, MEXICO.

USGS Publications Warehouse

Southworth, C. Scott; ,

1984-01-01

Landsat and Seasat satellite images and aerial photographs of eastern Yucatan Peninsula, Mexico, were analyzed to delineate geologic controls of ground water. Significant interpretation results include the delineation of linear topographic swales, interpreted as fractures, extending more than 50 km along strike from the previously known limit of the Holbox fracture system; the alignment of sink holes (cenotes) and inlets (caletas) on strike with existing faults and fracture systems; and the identification of tonal anomalies in Ingles Lagoon suggesting fresh-water discharge from a submarine spring.

Delineation of major geologic structures in Turkey using SIR-B data

NASA Technical Reports Server (NTRS)

Toksoz, M. N.; Pettengill, G. H.; Ford, P.; Gulen, L.

1984-01-01

Shuttle Imaging Radar-B (SIR-B) images of well mapped segments of major faults, such as the North Anatolian Fault (NAF) and East Anatolian Fault (EAF) will be studied to identify the prominent signatures that characterize the fault zones for those specific regions. The information will be used to delineate the unmapped fault zones in areas with similar geological and geomorphological properties. The data obtained from SIR-B images will be compared and correlated with the LANDSAT thematic mapper and seismicity alignments based on well constrained earthquake epicenters.

Mapping of neuron soma size as an effective approach to delineate differences between neural populations.

PubMed

Lingley, Alexander J; Bowdridge, Joshua C; Farivar, Reza; Duffy, Kevin R

2018-04-30

A single histological marker applied to a slice of tissue often reveals myriad cytoarchitectonic characteristics that can obscure differences between neuron populations targeted for study. Isolation and measurement of a single feature from the tissue is possible through a variety of approaches, however, visualizing the data numerically or through graphs alone can preclude being able to identify important features and effects that are not obvious from direct observation of the tissue. We demonstrate an efficient, effective, and robust approach to quantify and visualize cytoarchitectural features in histologically prepared brain sections. We demonstrate that this approach is able to reveal small differences between populations of neurons that might otherwise have gone undiscovered. We used stereological methods to record the cross-sectional soma area and in situ position of neurons within sections of the cat, monkey, and human visual system. The two-dimensional coordinate of every measured cell was used to produce a scatter plot that recapitulated the natural spatial distribution of cells, and each point in the plot was color-coded according to its respective soma area. The final graphic display was a multi-dimensional map of neuron soma size that revealed subtle differences across neuron aggregations, permitted delineation of regional boundaries, and identified small differences between populations of neurons modified by a period of sensory deprivation. This approach to collecting and displaying cytoarchitectonic data is simple, efficient, and provides a means of investigating small differences between neuron populations. Copyright © 2018. Published by Elsevier B.V.

Conservation genetics of the capercaillie in Poland - Delineation of conservation units.

PubMed

Rutkowski, Robert; Zawadzka, Dorota; Suchecka, Ewa; Merta, Dorota

2017-01-01

The capercaillie (Tetrao urogallus) is one of Poland's most endangered bird species, with an estimated population of 380-500 individuals in four isolated areas. To study these natural populations in Poland further, more than 900 non-invasive genetic samples were collected, along with samples from 59 birds representing large, continuous populations in Sweden and Russia; and from two centres in Poland breeding capercaillie. Microsatellite polymorphism at nine loci was then analysed to estimate within-population genetic diversity and genetic differentiation among populations. The results confirmed that isolation of populations and recent decreases in their sizes have reduced genetic diversity among capercaillie in Poland, with all the country's natural populations found to be experiencing the genetic after-effects of demographic bottlenecks. The results of analyses of genetic differentiation and structure further suggest the presence of a 'lowland' cluster (encompassing birds of the Augustowska and Solska Primaeval Forests in Poland, and of Sweden and Russia), and a Carpathian cluster. Capercaillie from Sweden and Russia are also found to differ markedly. The Polish lowland populations seem more closely related to birds from Scandinavia. Our genetic analysis also indicates that the stocks at breeding centres are of a high genetic diversity effectively reflecting the origins of founder individuals, though identification of ancestry requires further study in the case of some birds. Overall, the results sustain the conclusion that the Polish populations of capercaillie from the Carpathians and the lowlands should be treated as independent Management Units (MUs). This is to say that the breeding lines associated with these two sources should be maintained separately at breeding centres. The high level of genetic differentiation of birds from the Solska Primaeval Forest suggests that this population should also be assigned the status of independent MU.

Geomorphic mapping to support river restoration on the Trinity River downstream from Lewiston Dam, California, 1980-2011

USGS Publications Warehouse

Curtis, Jennifer A.; Guerrero, Timothy M.

2015-01-01

Historic land use, dam construction, water storage, and flow diversions in the Trinity River watershed have resulted in downstream geomorphic change, loss of salmonid habitat, and declines in salmonid populations. The USGS in cooperation with the Trinity River Restoration Program, a multi-agency partnership tasked with implementing federally mandated restoration, completed a geomorphic change assessment to inform the planning process for future restoration work. This report documents an ARCMAP geodatabase (v.10.0) containing geomorphic features digitized from a series of rectified orthophotographs (http://dx.doi.org/10.5066/F7TT4P04). Upland, riparian, and channel features were digitized from six available base images (1980, 1997, 2001, 2006, 2009, and 2011). This report describes the structure of the geodatabase and the methods used to delineate individual geomorphic features.

Reward magnitude tracking by neural populations in ventral striatum

PubMed Central

Fiallos, Ana M.; Bricault, Sarah J.; Cai, Lili X.; Worku, Hermoon A.; Colonnese, Matthew T.; Westmeyer, Gil; Jasanoff, Alan

2017-01-01

Evaluation of the magnitudes of intrinsically rewarding stimuli is essential for assigning value and guiding behavior. By combining parametric manipulation of a primary reward, medial forebrain bundle (MFB) microstimulation, with functional magnetic imaging (fMRI) in rodents, we delineated a broad network of structures activated by behaviorally characterized levels of rewarding stimulation. Correlation of psychometric behavioral measurements with fMRI response magnitudes revealed regions whose activity corresponded closely to the subjective magnitude of rewards. The largest and most reliable focus of reward magnitude tracking was observed in the shell region of the nucleus accumbens (NAc). Although the nonlinear nature of neurovascular coupling complicates interpretation of fMRI findings in precise neurophysiological terms, reward magnitude tracking was not observed in vascular compartments and could not be explained by saturation of region-specific hemodynamic responses. In addition, local pharmacological inactivation of NAc changed the profile of animals’ responses to rewards of different magnitudes without altering mean reward response rates, further supporting a hypothesis that neural population activity in this region contributes to assessment of reward magnitudes. PMID:27789262

Admixture patterns and genetic differentiation in negrito groups from West Malaysia estimated from genome-wide SNP data.

PubMed

Jinam, Timothy A; Phipps, Maude E; Saitou, Naruya

2013-01-01

Southeast Asia houses various culturally and linguistically diverse ethnic groups. In Malaysia, where the Malay, Chinese, and Indian ethnic groups form the majority, there exist minority groups such as the "negritos" who are believed to be descendants of the earliest settlers of Southeast Asia. Here we report patterns of genetic substructure and admixture in two Malaysian negrito populations (Jehai and Kensiu), using ~50,000 genome-wide single-nucleotide polymorphism (SNP) data. We found traces of recent admixture in both the negrito populations, particularly in the Jehai, with the Malay through principal component analysis and STRUCTURE analysis software, which suggested that the admixture was as recent as one generation ago. We also identified significantly differentiated nonsynonymous SNPs and haplotype blocks related to intracellular transport, metabolic processes, and detection of stimulus. These results highlight the different levels of admixture experienced by the two Malaysian negritos. Delineating admixture and differentiated genomic regions should be of importance in designing and interpretation of molecular anthropology and disease association studies. Copyright © 2013 Wayne State University Press, Detroit, Michigan 48201-1309.

36 CFR 212.1 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-07-01

... the National Forest System. Area. A discrete, specifically delineated space that is smaller, and in... including surface and shoulders, parking and side areas, structures, and such traffic-control devices as are...

36 CFR 212.1 - Definitions.

Code of Federal Regulations, 2011 CFR

2011-07-01

... the National Forest System. Area. A discrete, specifically delineated space that is smaller, and in... including surface and shoulders, parking and side areas, structures, and such traffic-control devices as are...

Variable rate application of nematicides on cotton fields: A promising site-specific management strategy.

USDA-ARS?s Scientific Manuscript database

Field tests were conducted to determine if differences in response to nematicide application (i.e., root-knot nematode (RKN) population levels, cotton yield, and profitability) occurred among RKN management zones (MZ). The MZ were delineated using variables related to soil texture, including appare...

Techniques for calling sapsuckers and finding their nesting territories

Treesearch

Francis M. Rushmore

1973-01-01

In 9 years of study in Maine, the author developed a survey method for estimating populations of yellow-bellied sapsuckers (Sphyrapicus varius varius L.) and delineating their territories. The birds were attracted for study by tapping hardwood dowels to imitate noises the birds make in drumming and feeding.

ELEMENTAL AND ISOTPOIC FINGERPRINTS OF JUVENILE WINTER FLOUNDER (PLEURONECTES AMERICANUS) FROM NARRAGANSETT BAY, RI (USA) AND SURROUNDING COASTAL PONDS

EPA Science Inventory

Elemental fingerprinting has become a powerful tool in fisheries science for identifying fish migration patterns, seasonal changes in habitat use, and for delineating the nursery origins of adult fish populations. In this study, we investigated whether elemental and isotopic sign...

A comparative study for image quality and radiation dose of a cone beam computed tomography scanner and a multislice computed tomography scanner for paranasal sinus imaging.

PubMed

De Cock, Jens; Zanca, Federica; Canning, John; Pauwels, Ruben; Hermans, Robert

2015-07-01

To evaluate image quality and radiation dose of a state of the art cone beam computed tomography (CBCT) system and a multislice computed tomography (MSCT) system in patients with sinonasal poliposis. In this retrospective study two radiologists evaluated 57 patients with sinonasal poliposis who underwent a CBCT or MSCT sinus examination, along with a control group of 90 patients with normal radiological findings. Tissue doses were measured using a phantom model with thermoluminescent dosimeters (TLD). Overall image quality in CBCT was scored significantly higher than in MSCT in patients with normal radiologic findings (p-value: 0.00001). In patients with sinonasal poliposis, MSCT scored significantly higher than CBCT (p-value: 0.00001). The average effective dose for MSCT was 42% higher compared to CBCT (108 μSv vs 63 μSv). CBCT and MSCT are both suited for the evaluation of sinonasal poliposis. In patients with sinonasal poliposis, clinically important structures of the paranasal sinuses can be better delineated with MSCT, whereas in patients without sinonasal poliposis, CBCT turns out to define the important structures of the sinonasal region better. However, given the lower radiation dose, CBCT can be considered for the evaluation of the sinonasal structures in patients with sinonasal poliposis. • CBCT and MSCT are both suited for evaluation of sinonasal poliposis. • Effective dose for MSCT was 42% higher compared to CBCT. • In patients with sinonasal poliposis, clinically important anatomical structures are better delineated with MSCT. • In patients with normal radiological findings, clinically important anatomical structures are better delineated with CBCT.

Analysis of Research Journals and Related Research Structure in Education. Final Report.

ERIC Educational Resources Information Center

Barron, Paul; Narin, Francis

This study delineates the structure of the educational journal literature, and its relationship to surrounding psychological journals. Citations amongst 140 heavily cited, widely circulating journals were used to define the interrelationships between the journals, and by inference between the research areas they represent. All education journals…

The landscape configuration of zoonotic transmission of Ebola virus disease in West and Central Africa: interaction between population density and vegetation cover.

PubMed

Walsh, Michael G; Haseeb, Ma

2015-01-01

Ebola virus disease (EVD) is an emerging infectious disease of zoonotic origin that has been responsible for high mortality and significant social disruption in West and Central Africa. Zoonotic transmission of EVD requires contact between susceptible human hosts and the reservoir species for Ebolaviruses, which are believed to be fruit bats. Nevertheless, features of the landscape that may facilitate such points of contact have not yet been adequately identified. Nor have spatial dependencies between zoonotic EVD transmission and landscape structures been delineated. This investigation sought to describe the spatial relationship between zoonotic EVD transmission events, or spillovers, and population density and vegetation cover. An inhomogeneous Poisson process model was fitted to all precisely geolocated zoonotic transmissions of EVD in West and Central Africa. Population density was strongly associated with spillover; however, there was significant interaction between population density and green vegetation cover. In areas of very low population density, increasing vegetation cover was associated with a decrease in risk of zoonotic transmission, but as population density increased in a given area, increasing vegetation cover was associated with increased risk of zoonotic transmission. This study showed that the spatial dependencies of Ebolavirus spillover were associated with the distribution of population density and vegetation cover in the landscape, even after controlling for climate and altitude. While this is an observational study, and thus precludes direct causal inference, the findings do highlight areas that may be at risk for zoonotic EVD transmission based on the spatial configuration of important features of the landscape.

Population genomics reveals seahorses (Hippocampus erectus) of the western mid-Atlantic coast to be residents rather than vagrants.

PubMed

Boehm, J T; Waldman, John; Robinson, John D; Hickerson, Michael J

2015-01-01

Understanding population structure and areas of demographic persistence and transients is critical for effective species management. However, direct observational evidence to address the geographic scale and delineation of ephemeral or persistent populations for many marine fishes is limited. The Lined seahorse (Hippocampus erectus) can be commonly found in three western Atlantic zoogeographic provinces, though inhabitants of the temperate northern Virginia Province are often considered tropical vagrants that only arrive during warm seasons from the southern provinces and perish as temperatures decline. Although genetics can locate regions of historical population persistence and isolation, previous evidence of Virginia Province persistence is only provisional due to limited genetic sampling (i.e., mitochondrial DNA and five nuclear loci). To test alternative hypotheses of historical persistence versus the ephemerality of a northern Virginia Province population we used a RADseq generated dataset consisting of 11,708 single nucleotide polymorphisms (SNP) sampled from individuals collected from the eastern Gulf of Mexico to Long Island, NY. Concordant results from genomic analyses all infer three genetically divergent subpopulations, and strongly support Virginia Province inhabitants as a genetically diverged and a historically persistent ancestral gene pool. These results suggest that individuals that emerge in coastal areas during the warm season can be considered "local" and supports offshore migration during the colder months. This research demonstrates how a large number of genes sampled across a geographical range can capture the diversity of coalescent histories (across loci) while inferring population history. Moreover, these results clearly demonstrate the utility of population genomic data to infer peripheral subpopulation persistence in difficult-to-observe species.

SU-C-BRA-05: Delineating High-Dose Clinical Target Volumes for Head and Neck Tumors Using Machine Learning Algorithms

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cardenas, C; The University of Texas Graduate School of Biomedical Sciences, Houston, TX; Wong, A

Purpose: To develop and test population-based machine learning algorithms for delineating high-dose clinical target volumes (CTVs) in H&N tumors. Automating and standardizing the contouring of CTVs can reduce both physician contouring time and inter-physician variability, which is one of the largest sources of uncertainty in H&N radiotherapy. Methods: Twenty-five node-negative patients treated with definitive radiotherapy were selected (6 right base of tongue, 11 left and 9 right tonsil). All patients had GTV and CTVs manually contoured by an experienced radiation oncologist prior to treatment. This contouring process, which is driven by anatomical, pathological, and patient specific information, typically results inmore » non-uniform margin expansions about the GTV. Therefore, we tested two methods to delineate high-dose CTV given a manually-contoured GTV: (1) regression-support vector machines(SVM) and (2) classification-SVM. These models were trained and tested on each patient group using leave-one-out cross-validation. The volume difference(VD) and Dice similarity coefficient(DSC) between the manual and auto-contoured CTV were calculated to evaluate the results. Distances from GTV-to-CTV were computed about each patient’s GTV and these distances, in addition to distances from GTV to surrounding anatomy in the expansion direction, were utilized in the regression-SVM method. The classification-SVM method used categorical voxel-information (GTV, selected anatomical structures, else) from a 3×3×3cm3 ROI centered about the voxel to classify voxels as CTV. Results: Volumes for the auto-contoured CTVs ranged from 17.1 to 149.1cc and 17.4 to 151.9cc; the average(range) VD between manual and auto-contoured CTV were 0.93 (0.48–1.59) and 1.16(0.48–1.97); while average(range) DSC values were 0.75(0.59–0.88) and 0.74(0.59–0.81) for the regression-SVM and classification-SVM methods, respectively. Conclusion: We developed two novel machine learning methods to delineate high-dose CTV for H&N patients. Both methods showed promising results that hint to a solution to the standardization of the contouring process of clinical target volumes. Varian Medical Systems grant.« less

Geochemical modeling of orogenic gold deposit using PCANN hybrid method in the Alut, Kurdistan province, Iran

NASA Astrophysics Data System (ADS)

Mohammadzadeh, Mohammadjafar; Nasseri, Aynur

2018-03-01

In this paper stream sediments based geochemical exploration program with the aim of delineating potentially promising areas by a comprehensive stepwise optimization approach from univariate statistics, PCA, ANN, and fusion method PCANN were under taken for an orogenic gold deposit located in the Alut, Kurdistan province, NW of Iran. At first the data were preprocessed and then PCA were applied to determine the maximum variability directions of elements in the area. Subsequently the artificial neural network (ANN) was used for quick estimation of elemental concentration, as well as discriminating anomalous populations and intelligent determination of internal structure among the data. However, both the methods revealed constraints for modeling. To overcome the deficiency and shortcoming of each individual method a new methodology is presented by integration of both "PCA & ANN" referred as PCANN method. For integrating purpose, the detected PCs pertinent to ore mineralization selected and intruded to neural network structure, as a result different MLPs with various algorithms and structures were produced. The resulting PCANN maps suggest that the gold mineralization and its pathfinder elements (Au, Mo, W, Bi, Sb, Cu, Pb, Ag & As) are associated with metamorphic host rocks intruded by granite bodies in the Alut area. In addition, more concealed and distinct Au anomalies with higher intensity were detected, confirming the privileges of the method in evaluating susceptibility of the area in delineating new hidden potential zones. The proposed method demonstrates simpler network architecture, easy computational implementation, faster training speed, as well as no need to consider any primary assumption about the behavior of data and their probability distribution type, with more satisfactory predicting performance for generating gold potential map of the area. Comparing the results of three methods (PCA, ANN and PCANN), representing the higher efficiency and more reliability of PCANN with lesser training time, simple structure, and correlate components while avoiding the duplicate entry of data to network. This study also suggests that in many similar cases integrated methods have capability to fix bugs more effectively and successfully in exploration programs.

R2 & NE Block Group - 2010 Census; Housing and Population Summary

EPA Pesticide Factsheets

The TIGER/Line Files are shapefiles and related database files (.dbf) that are an extract of selected geographic and cartographic information from the U.S. Census Bureau's Master Address File / Topologically Integrated Geographic Encoding and Referencing (MAF/TIGER) Database (MTDB). The MTDB represents a seamless national file with no overlaps or gaps between parts, however, each TIGER/Line File is designed to stand alone as an independent data set, or they can be combined to cover the entire nation. Block Groups (BGs) are defined before tabulation block delineation and numbering, but are clusters of blocks within the same census tract that have the same first digit of their 4-digit census block number from the same decennial census. For example, Census 2000 tabulation blocks 3001, 3002, 3003,.., 3999 within Census 2000 tract 1210.02 are also within BG 3 within that census tract. Census 2000 BGs generally contained between 600 and 3,000 people, with an optimum size of 1,500 people. Most BGs were delineated by local participants in the Census Bureau's Participant Statistical Areas Program (PSAP). The Census Bureau delineated BGs only where the PSAP participant declined to delineate BGs or where the Census Bureau could not identify any local PSAP participant. A BG usually covers a contiguous area. Each census tract contains at least one BG, and BGs are uniquely numbered within census tract. Within the standard census geographic hierarchy, BGs never cross

Analysis of individualized education programs to quantify long-term educational needs following surgical intervention for single-suture craniosynostosis.

PubMed

Doshier, Laura J; Muzaffar, Arshad R; Deidrick, Kathleen Km; Rice, Gale B

2015-01-01

Single-suture craniosynostosis (SSC) is a common craniofacial condition with potential neurocognitive sequelae. To quantify any long-term functional academic and behavioural difficulties of children with SSC as indicated by the need for individualized education programs (IEPs), despite having undergone surgical treatment. Records of all school-age patients from 1992 to 2011 who underwent operative intervention for SSC were identified. Fifty-nine patients' guardians were contacted by telephone to provide informed consent for completion of a mailed standardized questionnaire querying demographic information as well as information regarding the patient's health, family and educational history; specifically whether the patient had ever been provided educational support as delineated in an IEP. The primary outcome measure was the history of the patient being assigned educational support as delineated in an IEP. Thirty-seven consenting guardians completed and returned the standardized questionnaire (response rate 62.7%). Twenty-one patients were male and 16 were female, with an age range of five to 14 years (mean age 10.2 years). Eleven (29.7%) patients had a previous history of or currently were receiving educational support delineated in an IEP. A higher proportion of school-age patients with a history of SSC (status postsurgical intervention) in the present study received educational support delineated in an IEP than the proportion of IEPs in the general student population of the United States (11.3%).

Methods for Data-based Delineation of Spatial Regions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wilson, John E.

In data analysis, it is often useful to delineate or segregate areas of interest from the general population of data in order to concentrate further analysis efforts on smaller areas. Three methods are presented here for automatically generating polygons around spatial data of interest. Each method addresses a distinct data type. These methods were developed for and implemented in the sample planning tool called Visual Sample Plan (VSP). Method A is used to delineate areas of elevated values in a rectangular grid of data (raster). The data used for this method are spatially related. Although VSP uses data from amore » kriging process for this method, it will work for any type of data that is spatially coherent and appears on a regular grid. Method B is used to surround areas of interest characterized by individual data points that are congregated within a certain distance of each other. Areas where data are “clumped” together spatially will be delineated. Method C is used to recreate the original boundary in a raster of data that separated data values from non-values. This is useful when a rectangular raster of data contains non-values (missing data) that indicate they were outside of some original boundary. If the original boundary is not delivered with the raster, this method will approximate the original boundary.« less

Multilocus Sequence Typing of Bartonella henselae in the United Kingdom Indicates that Only a Few, Uncommon Sequence Types Are Associated with Zoonotic Disease▿†

PubMed Central

Chaloner, Gemma L.; Harrison, Timothy G.; Coyne, Karen P.; Aanensen, David M.; Birtles, Richard J.

2011-01-01

Bartonella henselae is one of the most common zoonotic agents acquired from companion animals (cats) in industrialized countries. Nonetheless, although the prevalence of infections in cats is high, the number of human cases reported is relatively low. One hypothesis for this discrepancy is that B. henselae strains vary in their zoonotic potential. To test this hypothesis, we employed structured sampling to explore the population structure of B. henselae in the United Kingdom and to determine the distribution of strains associated with zoonotic disease within this structure. A total of 118 B. henselae strains were delineated into 12 sequence types (STs) using multilocus sequence typing. We observed that most (85%) of the zoonosis-associated strains belonged to only three genotypes, i.e., ST2, ST5, and ST8. Conversely, most (74%) of the feline isolates belonged to ST4, ST6, and ST7. The difference in host association of ST2, ST5, and ST8 (zoonosis associated) and ST6 (feline) was statistically significant (P < 0.05), indicating that a few, uncommon STs were responsible for the majority of symptomatic human infections. PMID:21471345

Delineation of fault zones using imaging radar

NASA Technical Reports Server (NTRS)

Toksoz, M. N.; Gulen, L.; Prange, M.; Matarese, J.; Pettengill, G. H.; Ford, P. G.

1986-01-01

The assessment of earthquake hazards and mineral and oil potential of a given region requires a detailed knowledge of geological structure, including the configuration of faults. Delineation of faults is traditionally based on three types of data: (1) seismicity data, which shows the location and magnitude of earthquake activity; (2) field mapping, which in remote areas is typically incomplete and of insufficient accuracy; and (3) remote sensing, including LANDSAT images and high altitude photography. Recently, high resolution radar images of tectonically active regions have been obtained by SEASAT and Shuttle Imaging Radar (SIR-A and SIR-B) systems. These radar images are sensitive to terrain slope variations and emphasize the topographic signatures of fault zones. Techniques were developed for using the radar data in conjunction with the traditional types of data to delineate major faults in well-known test sites, and to extend interpretation techniques to remote areas.

Peregrine falcon

USGS Publications Warehouse

Skaggs, R.W.; Ellis, D.H.; Hunt, W.G.; Johnson, T.H.; Glinski, Richard L.; Pendleton, Beth Giron; Moss, Mary Beth; LeFranc, Maurice N.=; Millsap, Brian A.; Hoffman, Stephen W.

1988-01-01

Since 1975, 101 peregrine falcon breeding sites have been documented in Arizona, New Mexico and western Texas. Eyries were found in either riverine or montane canyons, habitats which supported dense and diverse avian prey and provided structural characteristics that may increase prey vulnerability. Approximately 85% of the sites visited each year were occupied. Egg-laying (mid-February to mid-May) typically occurred first in the south and at lower elevations, and later in the north and at higher elevations. Mean productivity of adult pairs (mean =1.55 young/year) was comparable to rates reported for healthy populations. Poor productivity and decreased territorial occupancy in western Texas and southeastern New Mexico occurred in the presence of high levels of environmental DDE. Estimates of the breeding population (141-187 occupied territories) are twice the U.S. Fish and Wildlife Service (USFWS) recovery goal for the region; however, the population is not considered recovered. The discrepancy of these assessments stems from the suitable-habitat approach used by the authors vs. the narrower eyrie-based USFWS recovery plan. Recommendations include reevaluation of recovery goals to ensure adequate habitat protection, elimination of regional sources of organochlorine pesticides, delineation of suitable habitat, formulation of habitat management prescriptions, winter habitat studies,continued monitoring of territorial occupancy and productivity, and accountability for sensitive information.

Detection of dead standing Eucalyptus camaldulensis without tree delineation for managing biodiversity in native Australian forest

NASA Astrophysics Data System (ADS)

Miltiadou, Milto; Campbell, Neil D. F.; Gonzalez Aracil, Susana; Brown, Tony; Grant, Michael G.

2018-05-01

In Australia, many birds and arboreal animals use hollows for shelters, but studies predict shortage of hollows in near future. Aged dead trees are more likely to contain hollows and therefore automated detection of them plays a substantial role in preserving biodiversity and consequently maintaining a resilient ecosystem. For this purpose full-waveform LiDAR data were acquired from a native Eucalypt forest in Southern Australia. The structure of the forest significantly varies in terms of tree density, age and height. Additionally, Eucalyptus camaldulensis have multiple trunk splits making tree delineation very challenging. For that reason, this paper investigates automated detection of dead standing Eucalyptus camaldulensis without tree delineation. It also presents the new feature of the open source software DASOS, which extracts features for 3D object detection in voxelised FW LiDAR. A random forest classifier, a weighted-distance KNN algorithm and a seed growth algorithm are used to create a 2D probabilistic field and to then predict potential positions of dead trees. It is shown that tree health assessment is possible without tree delineation but since it is a new research directions there are many improvements to be made.

Predator-prey trophic relationships in response to organic management practices.

PubMed

Schmidt, Jason M; Barney, Sarah K; Williams, Mark A; Bessin, Ricardo T; Coolong, Timothy W; Harwood, James D

2014-08-01

A broad range of environmental conditions likely regulate predator-prey population dynamics and impact the structure of these communities. Central to understanding the interplay between predator and prey populations and their importance is characterizing the corresponding trophic interactions. Here, we use a well-documented molecular approach to examine the structure of the community of natural enemies preying upon the squash bug, Anasa tristis, a herbivorous cucurbit pest that severely hinders organic squash and pumpkin production in the United States. Primer pairs were designed to examine the effects of organic management practices on the strength of these trophic connections and link this metric to measures of the arthropod predator complex density and diversity within an experimental open-field context. Replicated plots of butternut squash were randomly assigned to three treatments and were sampled throughout a growing season. Row-cover treatments had significant negative effects on squash bug and predator communities. In total, 640 predators were tested for squash bug molecular gut-content, of which 11% were found to have preyed on squash bugs, but predation varied over the season between predator groups (coccinellids, geocorids, nabids, web-building spiders and hunting spiders). Through the linking of molecular gut-content analysis to changes in diversity and abundance, these data delineate the complexity of interaction pathways on a pest that limits the profitability of organic squash production. © 2014 John Wiley & Sons Ltd.

It Takes Two: Contrasting Tasks and Contrasting Structures.

ERIC Educational Resources Information Center

Eisenstein, Miriam; And Others

1982-01-01

Examines and compares two measures of adult second language learner performance: cued production and elicited imitation. Discusses the utility of each in terms of the contrasting results of the tasks on a carefully delineated area of grammar, namely the related structure of third person simple present and present progressive in WH-questions. (EKN)

A method for polycrystalline silicon delineation applicable to a double-diffused MOS transistor

NASA Technical Reports Server (NTRS)

Halsor, J. L.; Lin, H. C.

1974-01-01

Method is simple and eliminates requirement for unreliable special etchants. Structure is graded in resistivity to prevent punch-through and has very narrow channel length to increase frequency response. Contacts are on top to permit planar integrated circuit structure. Polycrystalline shield will prevent creation of inversion layer in isolated region.

Writing To Learn in Science: A Curriculum Guide.

ERIC Educational Resources Information Center

Chatel, Regina G.

This curriculum guide supports and gives structure to engaging students in writing-to-learn activities in science classes by delineating writing outcomes and assessment. The guide is structured according to the beliefs that students need models, revision is the key to successful writing, writing is a tool for demonstrating learning, and writing is…

Unexpected absence of genetic separation of a highly diverse population of hookworms from geographically isolated hosts.

PubMed

Haynes, Benjamin T; Marcus, Alan D; Higgins, Damien P; Gongora, Jaime; Gray, Rachael; Šlapeta, Jan

2014-12-01

The high natal site fidelity of endangered Australian sea lions (Neophoca cinerea) along the southern Australian coast suggests that their maternally transmitted parasitic species, such as hookworms, will have restricted potential for dispersal. If this is the case, we would expect to find a hookworm haplotype structure corresponding to that of the host mtDNA haplotype structure; that is, restricted among geographically separated colonies. In this study, we used a fragment of the cytochrome c oxidase I mitochondrial DNA (mtDNA) gene to investigate the diversity of hookworms (Uncinaria sanguinis) in N. cinerea to assess the importance of host distribution and ecology on the evolutionary history of the parasite. High haplotype (h=0.986) and nucleotide diversity (π=0.013) were seen, with 45 unique hookworm mtDNA haplotypes across N. cinerea colonies; with most of the variation (78%) arising from variability within hookworms from individual colonies. This is supported by the low genetic differentiation co-efficient (GST=0.007) and a high gene flow (Nm=35.25) indicating a high migration rate between the populations of hookworms. The haplotype network demonstrated no clear distribution and delineation of haplotypes according to geographical location. Our data rejects the vicariance hypothesis; that female host natal site fidelity and the transmammary route of infection restrict hookworm gene flow between N. cinerea populations and highlights the value of studies of parasite diversity and dispersal to challenge our understanding of parasite and host ecology. Copyright © 2014 Elsevier B.V. All rights reserved.

32 CFR 179.5 - Responsibilities.

Code of Federal Regulations, 2012 CFR

2012-07-01

... rule. Upon further delineation and characterization of an MRA into more than one MRS, Components shall reapply the rule to all MRSs within the MRA. In such cases where data are not sufficient to populate one... modules. (b) Ensure that the total acreage of each MRA is evaluated using this rule (i.e., ensure the all...

32 CFR 179.5 - Responsibilities.

Code of Federal Regulations, 2014 CFR

2014-07-01

... rule. Upon further delineation and characterization of an MRA into more than one MRS, Components shall reapply the rule to all MRSs within the MRA. In such cases where data are not sufficient to populate one... modules. (b) Ensure that the total acreage of each MRA is evaluated using this rule (i.e., ensure the all...

32 CFR 179.5 - Responsibilities.

Code of Federal Regulations, 2013 CFR

2013-07-01

... rule. Upon further delineation and characterization of an MRA into more than one MRS, Components shall reapply the rule to all MRSs within the MRA. In such cases where data are not sufficient to populate one... modules. (b) Ensure that the total acreage of each MRA is evaluated using this rule (i.e., ensure the all...

Counseling through Images: Using Photography to Guide the Counseling Process and Achieve Treatment Goals

ERIC Educational Resources Information Center

Ginicola, Misty M.; Smith, Cheri; Trzaska, Jessica

2012-01-01

Creative approaches to counseling help counselors to meet the needs of diverse populations. The utility of photography in counseling has been demonstrated through several case studies; however, clear implications of how photography relates to the counseling process have not been well delineated. The existing literature on phototherapy is reviewed…

Creating Culturally Relevant Alzheimer's Support Groups for Racial and Ethnic Minorities.

ERIC Educational Resources Information Center

Henderson, Joseph Neil

Although data indicate that Alzheimer's disease occurs among all racial and ethnic populations, the Alzheimer's disease support group system is used nationally primarily by white, middle-class caregivers. Developing a model ethnic-specific support group for Hispanics requires delineation of formal and informal health care networks in the ethnic…

78 FR 9410 - Ozark Plateau National Wildlife Refuge; Adair, Cherokee, Craig, Delaware, Mayes, Ottawa, and...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-08

... 5: Wind Energy Farms. data of bird/bat migration populations corridors; use GIS affected by wind to delineate high- turbines and risk areas; determine locations quantify impacts; to minimize impacts...; improve vehicles; excess road with gravel parking near the from county road to maintenance shop...

Associating seasonal range characteristics with survival of female white-tailed deer

USGS Publications Warehouse

Klaver, R.W.; Jenks, J.A.; Deperno, C.S.; Griffin, S.L.

2008-01-01

Delineating populations is critical for understanding population dynamics and managing habitats. Our objective was to delineate subpopulations of migratory female white-tailed deer (Odocoileus virginianus) in the central Black Hills, South Dakota and Wyoming, USA, on summer and winter ranges. We used fuzzy classification to assign radiocollared deer to subpopulations based on spatial location, characterized subpopulations by trapping sites, and explored relationships among survival of subpopulations and habitat variables. In winter, Kaplan-Meier estimates for subpopulations indicated 2 groups: high (S = 0.991 ?? 0.005 [x- ?? SE]) and low (S = 0.968 ?? 0.007) weekly survivorship. Survivorship increased with basal area per hectare of trees, average diameter at breast height of trees, percent cover of slash, and total point-center quarter distance of trees. Cover of grass and forbs were less for the high survivorship than the lower survivorship group. In summer, deer were spaced apart with mixed associations among subpopulations. Habitat manipulations that promote or maintain large trees (i.e., basal area = 14.8 m2/ha and average dbh of trees = 8.3 cm) would seem to improve adult survival of deer in winter.

Longitudinal assessment of psychopathological domains over late-stage schizophrenia in relation to duration of initially untreated psychosis: 3-year prospective study in a long-term inpatient population.

PubMed

Meagher, David J; Quinn, John F; Bourke, Stephanie; Linehan, Sally; Murphy, Patrice; Kinsella, Anthony; Mullaney, James; Waddington, John L

2004-05-30

There remains uncertainty regarding any progressive nature of psychopathology and cognitive dysfunction in late-stage schizophrenia, and whether duration of initially untreated psychosis (DUP) might be associated with such 'progression'. This study examines longitudinally, over 3 years, the psychopathology and neuropsychology in 82 inpatients with DSM-IV schizophrenia, many of whom were admitted in the pre-neuroleptic era. Increase in executive dysfunction exceeded that in general cognitive impairment. Positive but not negative symptom severity decreased modestly; the primary predictor of negative symptom severity was DUP. On index assessment, psychopathology evidenced a three-factor structure; at follow-up, psychomotor poverty evidenced greater prominence and cohesion, and was on both occasions predicted primarily by DUP, while reality distortion was altered and disorganisation disassembled into alternative elements. It would appear that as years of chronic, refractory illness accrue, psychomotor poverty becomes more sharply delineated and dominant within the overall structure of psychopathology, and its prominence is predicted enduringly by DUP. Copyright 2004 Elsevier Ireland Ltd.

The Structure of Pre-Adolescents' Perceptions of Their Teacher's Interpersonal Behaviours and Their Relation to Pre-Adolescents' Learning Outcomes

ERIC Educational Resources Information Center

Charalampous, Kyriakos; Kokkinos, Constantinos M.

2018-01-01

Previous studies have offered indications that the way pre-adolescents (fifth and sixth graders) structure their perceptions of their teacher's interaction in terms of Agency and Communion differs from adolescents. The purpose of this study was to delineate previous findings by thoroughly examining the structure of pre-adolescents' perceptions of…

Utilizing Gravity Methods for Regional Studies in Basin Delineation: Case Study at Jornada del Muerto basin, New Mexico

NASA Astrophysics Data System (ADS)

Villalobos, J. I.

2005-12-01

The modeling of basin structures is an important step in the development of plans and policies for ground water management. To facilitate in the analysis of large scale regional structures, gravity data is implemented to examine the overall structural trend of the region. The gravitational attraction of structures in the upper mantle and crust provide vital information about the possible structure and composition of a region. Improved availability of gravity data via internet has promoted extensive construction and interpretation of gravity maps in the analysis of sub-surface structural anomalies. The utilization of gravity data appears to be particularly worthwhile because it is a non-invasive and inexpensive means of addressing the subsurface tectonic framework of large scale regions. In this paper, the author intends to illustrate 1) acquisition of gravity data and its processing; 2) interpretation of gravity data; and 3) sources of uncertainty and errors by using a case study of the Jornada del Muerto basin in South-Central New Mexico where integrated gravity data inferred several faults, sub-basins and thickness variations within the basins structure. The author also explores the integration of gravity method with other geophysical methods to further refine the delineation of basins.

Collective synchronization of self/non-self discrimination in T cell activation, across multiple spatio-temporal scales

NASA Astrophysics Data System (ADS)

Altan-Bonnet, Gregoire

The immune system is a collection of cells whose function is to eradicate pathogenic infections and malignant tumors while protecting healthy tissues. Recent work has delineated key molecular and cellular mechanisms associated with the ability to discriminate self from non-self agents. For example, structural studies have quantified the biophysical characteristics of antigenic molecules (those prone to trigger lymphocyte activation and a subsequent immune response). However, such molecular mechanisms were found to be highly unreliable at the individual cellular level. We will present recent efforts to build experimentally validated computational models of the immune responses at the collective cell level. Such models have become critical to delineate how higher-level integration through nonlinear amplification in signal transduction, dynamic feedback in lymphocyte differentiation and cell-to-cell communication allows the immune system to enforce reliable self/non-self discrimination at the organism level. In particular, we will present recent results demonstrating how T cells tune their antigen discrimination according to cytokine cues, and how competition for cytokine within polyclonal populations of cells shape the repertoire of responding clones. Additionally, we will present recent theoretical and experimental results demonstrating how competition between diffusion and consumption of cytokines determine the range of cell-cell communications within lymphoid organs. Finally, we will discuss how biochemically explicit models, combined with quantitative experimental validation, unravel the relevance of new feedbacks for immune regulations across multiple spatial and temporal scales.

An American termite in Paris: temporal colony dynamics.

PubMed

Baudouin, Guillaume; Dedeine, Franck; Bech, Nicolas; Bankhead-Dronnet, Stéphanie; Dupont, Simon; Bagnères, Anne-Geneviève

2017-12-01

Termites of the genus Reticulitermes are widespread invaders, particularly in urban habitats. Their cryptic and subterranean lifestyle makes them difficult to detect, and we know little about their colony dynamics over time. In this study we examined the persistence of Reticulitermes flavipes (Kollar) colonies in the city of Paris over a period of 15 years. The aim was (1) to define the boundaries of colonies sampled within the same four areas over two sampling periods, (2) to determine whether the colonies identified during the first sampling period persisted to the second sampling period, and (3) to compare the results obtained when colonies were delineated using a standard population genetic approach versus a Bayesian clustering method that combined both spatial and genetic information. Herein, colony delineations were inferred from genetic differences at nine microsatellite loci and one mitochondrial locus. Four of the 18 identified colonies did not show significant differences in their genotype distributions between the two sampling periods. While allelic richness was low, making it hard to reliably distinguish colony family type, most colonies appeared to retain the same breeding structure over time. These large and expansive colonies showed an important ability to fuse (39% were mixed-family colonies), contained hundreds of reproductives and displayed evidence of isolation-by-distance, suggesting budding dispersal. These traits, which favor colony persistence over time, present a challenge for pest control efforts, which apply treatment locally. The other colonies showed significant differences, but we cannot exclude the possibility that their genotype distributions simply changed over time.

Population Expansion and Genetic Structure in Carcharhinus brevipinna in the Southern Indo-Pacific

PubMed Central

Geraghty, Pascal T.; Williamson, Jane E.; Macbeth, William G.; Wintner, Sabine P.; Harry, Alastair V.; Ovenden, Jennifer R.; Gillings, Michael R.

2013-01-01

Background Quantifying genetic diversity and metapopulation structure provides insights into the evolutionary history of a species and helps develop appropriate management strategies. We provide the first assessment of genetic structure in spinner sharks (Carcharhinus brevipinna), a large cosmopolitan carcharhinid, sampled from eastern and northern Australia and South Africa. Methods and Findings Sequencing of the mitochondrial DNA NADH dehydrogenase subunit 4 gene for 430 individuals revealed 37 haplotypes and moderately high haplotype diversity (h = 0.6770 ±0.025). While two metrics of genetic divergence (ΦST and F ST) revealed somewhat different results, subdivision was detected between South Africa and all Australian locations (pairwise ΦST, range 0.02717–0.03508, p values ≤ 0.0013; pairwise F ST South Africa vs New South Wales = 0.04056, p = 0.0008). Evidence for fine-scale genetic structuring was also detected along Australia’s east coast (pairwise ΦST = 0.01328, p < 0.015), and between south-eastern and northern locations (pairwise ΦST = 0.00669, p < 0.04). Conclusions The Indian Ocean represents a robust barrier to contemporary gene flow in C. brevipinna between Australia and South Africa. Gene flow also appears restricted along a continuous continental margin in this species, with data tentatively suggesting the delineation of two management units within Australian waters. Further sampling, however, is required for a more robust evaluation of the latter finding. Evidence indicates that all sampled populations were shaped by a substantial demographic expansion event, with the resultant high genetic diversity being cause for optimism when considering conservation of this commercially-targeted species in the southern Indo-Pacific. PMID:24086462

Conservation genetics of snowy plovers (Charadrius alexandrinus) in the Western Hemisphere: Population genetic structure and delineation of subspecies

USGS Publications Warehouse

Funk, W.C.; Mullins, T.D.; Haig, S.M.

2007-01-01

We examined the genetic structure of snowy plovers (Charadrius alexandrinus) in North America, the Caribbean, and the west coast of South America to quantify variation within and among breeding areas and to test the validity of three previously recognized subspecies. Sequences (676 bp) from domains I and II of the mitochondrial control region were analyzed for 166 snowy plovers from 20 breeding areas. Variation was also examined at 10 microsatellite loci for 144 snowy plovers from 14 breeding areas. The mtDNA and microsatellite data provided strong evidence that the Puerto Rican breeding group is genetically divergent from sites in the continental U.S. (net sequence divergence = 0.38%; F ST for microsatellites = 0.190). Our data also revealed high levels of differentiation between sites from South America and North America (net sequence divergence = 0.81%; F ST for microsatellites = 0.253). In contrast, there was little genetic structure among breeding sites within the continental U.S. Our results suggest that snowy plovers in Florida should be considered part of C. a. nivosus (rather than part of C. a. tenuirostris, where they are currently placed), whereas snowy plovers from Puerto Rico should be considered part of C. a. tenuirostris. Snowy plovers in South America should remain a separate subspecies (C. a. occidentalis). Although U.S. Pacific and Gulf Coast breeding areas were not genetically distinct from other continental U.S. sites, demographic isolation, unique coastal habitats, and recent population declines suggest they warrant special concern. ?? 2007 Springer Science+Business Media, Inc.

Biology Teachers' Perceptions of Subject Matter Structure and Its Relationship to Classroom Practice.

ERIC Educational Resources Information Center

Gess-Newsome, Julie; Lederman, Norman G.

Current reform efforts in the teaching of high school biology demonstrate the need for a synthetic treatment of prominent concepts. There exists insufficient research that delineates the global content understandings--in this paper designated subject matter structures (SMS)--of biology teachers; or that assesses whether these SMS do, in fact,…

Interaction Structures between a Child and Two Therapists in the Psychodynamic Treatment of a Child with Asperger's Disorder

ERIC Educational Resources Information Center

Goodman, Geoff; Athey-Lloyd, Laura

2011-01-01

Leading the charge to link intervention research with clinical practice is the development of process research, which involves a detailed analysis of specific therapeutic processes over the course of treatment. The delineation of interaction structures--repetitive patterns of interactions between patient and therapist over the course of…

Effects of Changed Family Structures on Children: A Review of the Literature.

ERIC Educational Resources Information Center

Kendall, Earline D., Ed.; And Others

Current changes in the American nuclear family, the impact of changes in family structure on children, and a rationale for day care services are delineated in this literature review. The family modifications examined are (1) divorce, (2) remarriage, (3) single parenting, (4) father absence, (5) teenage parenting, and (6) extended families.…

Population Genomics Reveals Seahorses (Hippocampus erectus) of the Western Mid-Atlantic Coast to Be Residents Rather than Vagrants

PubMed Central

Boehm, J. T.; Waldman, John; Robinson, John D.; Hickerson, Michael J.

2015-01-01

Understanding population structure and areas of demographic persistence and transients is critical for effective species management. However, direct observational evidence to address the geographic scale and delineation of ephemeral or persistent populations for many marine fishes is limited. The Lined seahorse (Hippocampus erectus) can be commonly found in three western Atlantic zoogeographic provinces, though inhabitants of the temperate northern Virginia Province are often considered tropical vagrants that only arrive during warm seasons from the southern provinces and perish as temperatures decline. Although genetics can locate regions of historical population persistence and isolation, previous evidence of Virginia Province persistence is only provisional due to limited genetic sampling (i.e., mitochondrial DNA and five nuclear loci). To test alternative hypotheses of historical persistence versus the ephemerality of a northern Virginia Province population we used a RADseq generated dataset consisting of 11,708 single nucleotide polymorphisms (SNP) sampled from individuals collected from the eastern Gulf of Mexico to Long Island, NY. Concordant results from genomic analyses all infer three genetically divergent subpopulations, and strongly support Virginia Province inhabitants as a genetically diverged and a historically persistent ancestral gene pool. These results suggest that individuals that emerge in coastal areas during the warm season can be considered “local” and supports offshore migration during the colder months. This research demonstrates how a large number of genes sampled across a geographical range can capture the diversity of coalescent histories (across loci) while inferring population history. Moreover, these results clearly demonstrate the utility of population genomic data to infer peripheral subpopulation persistence in difficult-to-observe species. PMID:25629166

Analysis of individualized education programs to quantify long-term educational needs following surgical intervention for single-suture craniosynostosis

PubMed Central

Doshier, Laura J; Muzaffar, Arshad R; Deidrick, Kathleen KM; Rice, Gale B

2015-01-01

BACKGROUND: Single-suture craniosynostosis (SSC) is a common craniofacial condition with potential neurocognitive sequelae. OBJECTIVE: To quantify any long-term functional academic and behavioural difficulties of children with SSC as indicated by the need for individualized education programs (IEPs), despite having undergone surgical treatment. METHODS: Records of all school-age patients from 1992 to 2011 who underwent operative intervention for SSC were identified. Fifty-nine patients’ guardians were contacted by telephone to provide informed consent for completion of a mailed standardized questionnaire querying demographic information as well as information regarding the patient’s health, family and educational history; specifically whether the patient had ever been provided educational support as delineated in an IEP. The primary outcome measure was the history of the patient being assigned educational support as delineated in an IEP. RESULTS: Thirty-seven consenting guardians completed and returned the standardized questionnaire (response rate 62.7%). Twenty-one patients were male and 16 were female, with an age range of five to 14 years (mean age 10.2 years). Eleven (29.7%) patients had a previous history of or currently were receiving educational support delineated in an IEP. CONCLUSIONS: A higher proportion of school-age patients with a history of SSC (status postsurgical intervention) in the present study received educational support delineated in an IEP than the proportion of IEPs in the general student population of the United States (11.3%). PMID:25821770

A comparison of three fiber tract delineation methods and their impact on white matter analysis.

PubMed

Sydnor, Valerie J; Rivas-Grajales, Ana María; Lyall, Amanda E; Zhang, Fan; Bouix, Sylvain; Karmacharya, Sarina; Shenton, Martha E; Westin, Carl-Fredrik; Makris, Nikos; Wassermann, Demian; O'Donnell, Lauren J; Kubicki, Marek

2018-05-19

Diffusion magnetic resonance imaging (dMRI) is an important method for studying white matter connectivity in the brain in vivo in both healthy and clinical populations. Improvements in dMRI tractography algorithms, which reconstruct macroscopic three-dimensional white matter fiber pathways, have allowed for methodological advances in the study of white matter; however, insufficient attention has been paid to comparing post-tractography methods that extract white matter fiber tracts of interest from whole-brain tractography. Here we conduct a comparison of three representative and conceptually distinct approaches to fiber tract delineation: 1) a manual multiple region of interest-based approach, 2) an atlas-based approach, and 3) a groupwise fiber clustering approach, by employing methods that exemplify these approaches to delineate the arcuate fasciculus, the middle longitudinal fasciculus, and the uncinate fasciculus in 10 healthy male subjects. We enable qualitative comparisons across methods, conduct quantitative evaluations of tract volume, tract length, mean fractional anisotropy, and true positive and true negative rates, and report measures of intra-method and inter-method agreement. We discuss methodological similarities and differences between the three approaches and the major advantages and drawbacks of each, and review research and clinical contexts for which each method may be most apposite. Emphasis is given to the means by which different white matter fiber tract delineation approaches may systematically produce variable results, despite utilizing the same input tractography and reliance on similar anatomical knowledge. Copyright © 2018. Published by Elsevier Inc.

Electronegativity and hardness as coordinates in structure stability diagrams.

PubMed Central

Shankar, S; Parr, R G

1985-01-01

With electronegativity and hardness of an atom defined as 1/2(I + A) and 1/2(I - A), respectively, where I and A are the ionization potential and electron affinity, electronegativity difference and hardness sum are proposed as coordinates in structure stability diagrams. With these coordinates a successful topological classification of the crystal structures of octet and suboctet binary compounds is obtained, and a clear delineation of the structural classes portraying chemical periodicity is found. PMID:3855552

Disorder-Specific Effects of CBT for Anxious and Depressed Youth: A Meta-Analysis of Candidate Mediators of Change

ERIC Educational Resources Information Center

Chu, Brian C.; Harrison, Tara L.

2007-01-01

The commonalities between anxiety and depression have been discussed before, but few have delineated the potentially different mechanisms through which treatments work for these populations. The current study conducted a comprehensive review of child and adolescent randomized clinical trials that tested cognitive-behavioral therapy (CBT) for…

GSD Update: Ushering in a new age of genetics to restore lands and conserve species

Treesearch

Deborah M. Finch

2013-01-01

Plant genetic information provides critical knowledge necessary to mitigate the impacts of climate change through ecological restoration. The first step in restoration is recognizing and delineating genetic boundaries at different taxonomic and spatial hierarchies (e.g., species, subspecies and populations). The second step is an assessment of the genetic diversity...

Polycross populations of the native grass Festuca roemeri as pre-varietal germplasm: their derivation, release, increase, and use

Treesearch

Dale C. Darris; Barbara L. Wilson; Rob Fiegener; Matthew E. Horning

2008-01-01

Results of a recent common-garden study provide evidence needed to delineate appropriate seed transfer zones for the native grass Festuca roemeri (Pavlick) E. B. Alexeev (Poaceae). That information has been used to develop pre-variety germplasm releases to provide ecologically and genetically appropriate seeds for habitat restoration, erosion...

Molecular detection of northern leatherside chub (Lepidomeda copei) DNA in environmental samples

Treesearch

Joseph C. Dysthe; Kellie J. Carim; Thomas W. Franklin; Dave Kikkert; Michael K. Young; Kevin S. McKelvey; Michael K. Schwartz

2018-01-01

The northern leatherside chub (Lepidomeda copei) is a cyprinid fish native to the Snake River, Green River, and Bonneville basins of the western United States. Population declines prompted the development of a multistate conservation agreement and strategy, which emphasized the need to reliably delineate its current distribution and monitor its status. To facilitate...

Assessing Attachment with the Bird's Nest Drawing: A Review of the Research

ERIC Educational Resources Information Center

Kaiser, Donna H.; Deaver, Sarah

2009-01-01

The Bird's Nest Drawing (Kaiser, 1996) is an art-based assessment that was developed to assess attachment security. In the past 10 years, several studies have further tested this art therapy directive with various populations. This paper briefly reviews attachment theory, delineates the significant findings from five of the studies, and provides…

The Impact of Nonverbal Ability on Prevalence and Clinical Presentation of Language Disorder: Evidence from a Population Study

ERIC Educational Resources Information Center

Norbury, Courtenay Frazier; Gooch, Debbie; Wray, Charlotte; Baird, Gillian; Charman, Tony; Simonoff, Emily; Vamvakas, George; Pickles, Andrew

2016-01-01

Background: Diagnosis of "specific" language impairment traditionally required nonverbal IQ to be within normal limits, often resulting in restricted access to clinical services for children with lower NVIQ. Changes to DSM-5 criteria for language disorder removed this NVIQ requirement. This study sought to delineate the impact of varying…

Mexican Americans, Chicanos, and Others: Ethnic Self-Identification and Selected Social Attributes of Rural Texas Youth

ERIC Educational Resources Information Center

Miller, Michael V.

1976-01-01

Following the thesis that variations in ethnic identification reflect social differentiation within the Mexican American population, this paper sought to: (1) delineate primary terms for ethnic self-identification among youths residing in a relatively homogeneous area of South Texas, (2) test the generalizability of past findings, and (3) examine…

Self-Reported Mood, General Health, Wellbeing and Employment Status in Adults with Suspected DCD

ERIC Educational Resources Information Center

Kirby, Amanda; Williams, Natalie; Thomas, Marie; Hill, Elisabeth L.

2013-01-01

Developmental Coordination Disorder (DCD) affects around 2-6% of the population and is diagnosed on the basis of poor motor coordination in the absence of other neurological disorders. Its psychosocial impact has been delineated in childhood but until recently there has been little understanding of the implications of the disorder beyond this.…

Social Contextual Links to Emotion Regulation in an Adolescent Psychiatric Inpatient Population: Do Gender and Symptomatology Matter?

ERIC Educational Resources Information Center

Adrian, Molly; Zeman, Janice; Erdley, Cynthia; Lisa, Ludmila; Homan, Kendra; Sim, Leslie

2009-01-01

Background: The regulation of emotion is essential for adaptive functioning. However, delineating the pathways of emotion regulation (ER) processes that lead to psychological adaptation remains under-studied, with mixed evidence for the specificity vs. generality of ER deficits in relation to specific forms of psychopathology. To examine this…

Hydrogeologic framework of sedimentary deposits in six structural basins, Yakima River basin, Washington

USGS Publications Warehouse

Jones, M.A.; Vaccaro, J.J.; Watkins, A.M.

2006-01-01

The hydrogeologic framework was delineated for the ground-water flow system of the sedimentary deposits in six structural basins in the Yakima River Basin, Washington. The six basins delineated, from north to south are: Roslyn, Kittitas, Selah, Yakima, Toppenish, and Benton. Extent and thicknesses of the hydrogeologic units and total basin sediment thickness were mapped for each basin. Interpretations were based on information from about 4,700 well records using geochemical, geophysical, geologist's or driller's logs, and from the surficial geology and previously constructed maps and well interpretations. The sedimentary deposits were thickest in the Kittitas Basin reaching a depth of greater than 2,000 ft, followed by successively thinner sedimentary deposits in the Selah basin with about 1,900 ft, Yakima Basin with about 1,800 ft, Toppenish Basin with about 1,200 ft, Benton basin with about 870 ft and Roslyn Basin with about 700 ft.

Automatic anatomy recognition via multiobject oriented active shape models.

PubMed

Chen, Xinjian; Udupa, Jayaram K; Alavi, Abass; Torigian, Drew A

2010-12-01

This paper studies the feasibility of developing an automatic anatomy recognition (AAR) system in clinical radiology and demonstrates its operation on clinical 2D images. The anatomy recognition method described here consists of two main components: (a) multiobject generalization of OASM and (b) object recognition strategies. The OASM algorithm is generalized to multiple objects by including a model for each object and assigning a cost structure specific to each object in the spirit of live wire. The delineation of multiobject boundaries is done in MOASM via a three level dynamic programming algorithm, wherein the first level is at pixel level which aims to find optimal oriented boundary segments between successive landmarks, the second level is at landmark level which aims to find optimal location for the landmarks, and the third level is at the object level which aims to find optimal arrangement of object boundaries over all objects. The object recognition strategy attempts to find that pose vector (consisting of translation, rotation, and scale component) for the multiobject model that yields the smallest total boundary cost for all objects. The delineation and recognition accuracies were evaluated separately utilizing routine clinical chest CT, abdominal CT, and foot MRI data sets. The delineation accuracy was evaluated in terms of true and false positive volume fractions (TPVF and FPVF). The recognition accuracy was assessed (1) in terms of the size of the space of the pose vectors for the model assembly that yielded high delineation accuracy, (2) as a function of the number of objects and objects' distribution and size in the model, (3) in terms of the interdependence between delineation and recognition, and (4) in terms of the closeness of the optimum recognition result to the global optimum. When multiple objects are included in the model, the delineation accuracy in terms of TPVF can be improved to 97%-98% with a low FPVF of 0.1%-0.2%. Typically, a recognition accuracy of > or = 90% yielded a TPVF > or = 95% and FPVF < or = 0.5%. Over the three data sets and over all tested objects, in 97% of the cases, the optimal solutions found by the proposed method constituted the true global optimum. The experimental results showed the feasibility and efficacy of the proposed automatic anatomy recognition system. Increasing the number of objects in the model can significantly improve both recognition and delineation accuracy. More spread out arrangement of objects in the model can lead to improved recognition and delineation accuracy. Including larger objects in the model also improved recognition and delineation. The proposed method almost always finds globally optimum solutions.

Regional investigations of tectonic and igneous geology, Iran, Pakistan, and Turkey

NASA Technical Reports Server (NTRS)

1978-01-01

The author has identified the following significant results. An extension of the trace of the Chaman-Nushki fault was detected and delineated for 42 km, as was the Ornach-Nal fault for 170 km. Two structural intersections responsible for restricted movements in particular segments of the Chaman-Nushki fault were detected and interpreted. The newest and youngest fault named the Quetta-Mustung-Surab system was delineated for 580 km. The igneous complex of the Lasbela area was interpreted and differentiation was made between ultramafic complex, mafic complex, and basaltic lava flows. One oblong feature was also found which was interpreted as a porphyritic basalt plug.

Building the School Attendance Boundary Information System (SABINS): Collecting, Processing, and Modeling K to 12 Educational Geography

PubMed Central

Saporito, Salvatore; Van Riper, David; Wakchaure, Ashwini

2017-01-01

The School Attendance Boundary Information System is a social science data infrastructure project that assembles, processes, and distributes spatial data delineating K through 12th grade school attendance boundaries for thousands of school districts in U.S. Although geography is a fundamental organizing feature of K to 12 education, until now school attendance boundary data have not been made readily available on a massive basis and in an easy-to-use format. The School Attendance Boundary Information System removes these barriers by linking spatial data delineating school attendance boundaries with tabular data describing the demographic characteristics of populations living within those boundaries. This paper explains why a comprehensive GIS database of K through 12 school attendance boundaries is valuable, how original spatial information delineating school attendance boundaries is collected from local agencies, and techniques for modeling and storing the data so they provide maximum flexibility to the user community. An important goal of this paper is to share the techniques used to assemble the SABINS database so that local and state agencies apply a standard set of procedures and models as they gather data for their regions. PMID:29151773

Building the School Attendance Boundary Information System (SABINS): Collecting, Processing, and Modeling K to 12 Educational Geography.

PubMed

Saporito, Salvatore; Van Riper, David; Wakchaure, Ashwini

2013-01-01

The School Attendance Boundary Information System is a social science data infrastructure project that assembles, processes, and distributes spatial data delineating K through 12 th grade school attendance boundaries for thousands of school districts in U.S. Although geography is a fundamental organizing feature of K to 12 education, until now school attendance boundary data have not been made readily available on a massive basis and in an easy-to-use format. The School Attendance Boundary Information System removes these barriers by linking spatial data delineating school attendance boundaries with tabular data describing the demographic characteristics of populations living within those boundaries. This paper explains why a comprehensive GIS database of K through 12 school attendance boundaries is valuable, how original spatial information delineating school attendance boundaries is collected from local agencies, and techniques for modeling and storing the data so they provide maximum flexibility to the user community. An important goal of this paper is to share the techniques used to assemble the SABINS database so that local and state agencies apply a standard set of procedures and models as they gather data for their regions.

An evaluation of automated GIS tools for delineating karst sinkholes and closed depressions from 1-meter LIDAR-derived digital elevation data

USGS Publications Warehouse

Doctor, Daniel H.; Young, John A.

2013-01-01

LiDAR (Light Detection and Ranging) surveys of karst terrains provide high-resolution digital elevation models (DEMs) that are particularly useful for mapping sinkholes. In this study, we used automated processing tools within ArcGIS (v. 10.0) operating on a 1.0 m resolution LiDAR DEM in order to delineate sinkholes and closed depressions in the Boyce 7.5 minute quadrangle located in the northern Shenandoah Valley of Virginia. The results derived from the use of the automated tools were then compared with depressions manually delineated by a geologist. Manual delineation of closed depressions was conducted using a combination of 1.0 m DEM hillshade, slopeshade, aerial imagery, and Topographic Position Index (TPI) rasters. The most effective means of visualizing depressions in the GIS was using an overlay of the partially transparent TPI raster atop the slopeshade raster at 1.0 m resolution. Manually identified depressions were subsequently checked using aerial imagery to screen for false positives, and targeted ground-truthing was undertaken in the field. The automated tools that were utilized include the routines in ArcHydro Tools (v. 2.0) for prescreening, evaluating, and selecting sinks and depressions as well as thresholding, grouping, and assessing depressions from the TPI raster. Results showed that the automated delineation of sinks and depressions within the ArcHydro tools was highly dependent upon pre-conditioning of the DEM to produce "hydrologically correct" surface flow routes. Using stream vectors obtained from the National Hydrologic Dataset alone to condition the flow routing was not sufficient to produce a suitable drainage network, and numerous artificial depressions were generated where roads, railways, or other manmade structures acted as flow barriers in the elevation model. Additional conditioning of the DEM with drainage paths across these barriers was required prior to automated 2delineation of sinks and depressions. In regions where the DEM had been properly conditioned, the tools for automated delineation performed reasonably well as compared to the manually delineated depressions, but generally overestimated the number of depressions thus necessitating manual filtering of the final results. Results from the TPI thresholding analysis were not dependent on DEM pre-conditioning, but the ability to extract meaningful depressions depended on careful assessment of analysis scale and TPI thresholding.

Delineation of early brain development from fetuses to infants with diffusion MRI and beyond.

PubMed

Ouyang, Minhui; Dubois, Jessica; Yu, Qinlin; Mukherjee, Pratik; Huang, Hao

2018-04-12

Dynamic macrostructural and microstructural changes take place from the mid-fetal stage to 2 years after birth. Delineating structural changes of the brain during early development provides new insights into the complicated processes of both typical development and the pathological mechanisms underlying various psychiatric and neurological disorders including autism, attention deficit hyperactivity disorder and schizophrenia. Decades of histological studies have identified strong spatial and functional maturation gradients in human brain gray and white matter. The recent improvements in magnetic resonance imaging (MRI) techniques, especially diffusion MRI (dMRI), relaxometry imaging, and magnetization transfer imaging (MTI) have provided unprecedented opportunities to non-invasively quantify and map the early developmental changes at whole brain and regional levels. Here, we review the recent advances in understanding early brain structural development during the second half of gestation and the first two postnatal years using modern MR techniques. Specifically, we review studies that delineate the emergence and microstructural maturation of white matter tracts, as well as dynamic mapping of inhomogeneous cortical microstructural organization unique to fetuses and infants. These imaging studies converge into maturational curves of MRI measurements that are distinctive across different white matter tracts and cortical regions. Furthermore, contemporary models offering biophysical interpretations of the dMRI-derived measurements are illustrated to infer the underlying microstructural changes. Collectively, this review summarizes findings that contribute to charting spatiotemporally heterogeneous gray and white matter structural development, offering MRI-based biomarkers of typical brain development and setting the stage for understanding aberrant brain development in neurodevelopmental disorders. Copyright © 2018 Elsevier Inc. All rights reserved.

Delineating ecological regions in marine systems: Integrating physical structure and community composition to inform spatial management in the eastern Bering Sea

NASA Astrophysics Data System (ADS)

Baker, Matthew R.; Hollowed, Anne B.

2014-11-01

Characterizing spatial structure and delineating meaningful spatial boundaries have useful applications to understanding regional dynamics in marine systems, and are integral to ecosystem approaches to fisheries management. Physical structure and drivers combine with biological responses and interactions to organize marine systems in unique ways at multiple scales. We apply multivariate statistical methods to define spatially coherent ecological units or ecoregions in the eastern Bering Sea. We also illustrate a practical approach to integrate data on species distribution, habitat structure and physical forcing mechanisms to distinguish areas with distinct biogeography as one means to define management units in large marine ecosystems. We use random forests to quantify the relative importance of habitat and environmental variables to the distribution of individual species, and to quantify shifts in multispecies assemblages or community composition along environmental gradients. Threshold shifts in community composition are used to identify regions with distinct physical and biological attributes, and to evaluate the relative importance of predictor variables to determining regional boundaries. Depth, bottom temperature and frontal boundaries were dominant factors delineating distinct biological communities in this system, with a latitudinal divide at approximately 60°N. Our results indicate that distinct climatic periods will shift habitat gradients and that dynamic physical variables such as temperature and stratification are important to understanding temporal stability of ecoregion boundaries. We note distinct distribution patterns among functional guilds and also evidence for resource partitioning among individual species within each guild. By integrating physical and biological data to determine spatial patterns in community composition, we partition ecosystems along ecologically significant gradients. This may provide a basis for defining spatial management units or serve as a baseline index for analyses of structural shifts in the physical environment, species abundance and distribution, and community dynamics over time.

A landscape genetic analysis of important agricultural pest species in Tunisia: The whitefly Bemisia tabaci

PubMed Central

Fakhfakh, Hatem; Belkadhi, Mohamed Sadok

2017-01-01

Combining landscape ecology and genetics provides an excellent framework to appreciate pest population dynamics and dispersal. The genetic architectures of many species are always shaped by environmental constraints. Because little is known about the ecological and genetic traits of Tunisian whitefly populations, the main objective of this work is to highlight patterns of biodiversity, genetic structure and migration routes of this pest. We used nuclear microsatellite loci to analyze B. tabaci populations collected from various agricultural areas across the country and we determine their biotype status. Molecular data were subsequently interpreted in an ecological context supplied from a species distribution model to infer habitat suitability and hereafter the potential connection paths between sampling localities. An analysis of landscape resistance to B. tabaci genetic flow was thus applied to take into account habitat suitability, genetic relatedness and functional connectivity of habitats within a varied landscape matrix. We shed light on the occurrence of three geographically delineated genetic groups with high levels of genetic differentiation within each of them. Potential migration corridors of this pest were then established providing significant advances toward the understanding of genetic features and the dynamic dispersal of this pest. This study supports the hypothesis of a long-distance dispersal of B. tabaci followed by infrequent long-term isolations. The Inference of population sources and colonization routes is critical for the design and implementation of accurate management strategies against this pest. PMID:28972992

A landscape genetic analysis of important agricultural pest species in Tunisia: The whitefly Bemisia tabaci.

PubMed

Ben Abdelkrim, Ahmed; Hattab, Tarek; Fakhfakh, Hatem; Belkadhi, Mohamed Sadok; Gorsane, Faten

2017-01-01

Combining landscape ecology and genetics provides an excellent framework to appreciate pest population dynamics and dispersal. The genetic architectures of many species are always shaped by environmental constraints. Because little is known about the ecological and genetic traits of Tunisian whitefly populations, the main objective of this work is to highlight patterns of biodiversity, genetic structure and migration routes of this pest. We used nuclear microsatellite loci to analyze B. tabaci populations collected from various agricultural areas across the country and we determine their biotype status. Molecular data were subsequently interpreted in an ecological context supplied from a species distribution model to infer habitat suitability and hereafter the potential connection paths between sampling localities. An analysis of landscape resistance to B. tabaci genetic flow was thus applied to take into account habitat suitability, genetic relatedness and functional connectivity of habitats within a varied landscape matrix. We shed light on the occurrence of three geographically delineated genetic groups with high levels of genetic differentiation within each of them. Potential migration corridors of this pest were then established providing significant advances toward the understanding of genetic features and the dynamic dispersal of this pest. This study supports the hypothesis of a long-distance dispersal of B. tabaci followed by infrequent long-term isolations. The Inference of population sources and colonization routes is critical for the design and implementation of accurate management strategies against this pest.

Primer selection impacts specific population abundances but not community dynamics in a monthly time-series 16S rRNA gene amplicon analysis of coastal marine bacterioplankton.

PubMed

Wear, Emma K; Wilbanks, Elizabeth G; Nelson, Craig E; Carlson, Craig A

2018-03-09

Primers targeting the 16S small subunit ribosomal RNA marker gene, used to characterize bacterial and archaeal communities, have recently been re-evaluated for marine planktonic habitats. To investigate whether primer selection affects the ecological interpretation of bacterioplankton populations and community dynamics, amplicon sequencing with four primer sets targeting several hypervariable regions of the 16S rRNA gene was conducted on both mock communities constructed from cloned 16S rRNA genes and a time-series of DNA samples from the temperate coastal Santa Barbara Channel. Ecological interpretations of community structure (delineation of depth and seasonality, correlations with environmental factors) were similar across primer sets, while population dynamics varied. We observed substantial differences in relative abundances of taxa known to be poorly resolved by some primer sets, such as Thaumarchaeota and SAR11, and unexpected taxa including Roseobacter clades. Though the magnitude of relative abundances of common OTUs differed between primer sets, the relative abundances of the OTUs were nonetheless strongly correlated. We do not endorse one primer set but rather enumerate strengths and weaknesses to facilitate selection appropriate to a system or experimental goal. While 16S rRNA gene primer bias suggests caution in assessing quantitative population dynamics, community dynamics appear robust across studies using different primers. © 2018 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.

R2 & NE Tract - 2010 Census; Housing and Population Summary

EPA Pesticide Factsheets

The TIGER/Line Files are shapefiles and related database files (.dbf) that are an extract of selected geographic and cartographic information from the U.S. Census Bureau's Master Address File / Topologically Integrated Geographic Encoding and Referencing (MAF/TIGER) Database (MTDB). The MTDB represents a seamless national file with no overlaps or gaps between parts, however, each TIGER/Line File is designed to stand alone as an independent data set, or they can be combined to cover the entire nation. Census tracts are small, relatively permanent statistical subdivisions of a county or equivalent entity, and were defined by local participants as part of the 2010 Census Participant Statistical Areas Program. The Census Bureau delineated the census tracts in situations where no local participant existed or where all the potential participants declined to participate. The primary purpose of census tracts is to provide a stable set of geographic units for the presentation of census data and comparison back to previous decennial censuses. Census tracts generally have a population size between 1,200 and 8,000 people, with an optimum size of 4,000 people. When first delineated, census tracts were designed to be homogeneous with respect to population characteristics, economic status, and living conditions. The spatial size of census tracts varies widely depending on the density of settlement. Physical changes in street patterns caused by highway construction, new

Pattern recognition receptor-mediated cytokine response in infants across 4 continents.

PubMed

Smolen, Kinga K; Ruck, Candice E; Fortuno, Edgardo S; Ho, Kevin; Dimitriu, Pedro; Mohn, William W; Speert, David P; Cooper, Philip J; Esser, Monika; Goetghebuer, Tessa; Marchant, Arnaud; Kollmann, Tobias R

2014-03-01

Susceptibility to infection as well as response to vaccination varies among populations. To date, the underlying mechanisms responsible for these clinical observations have not been fully delineated. Because innate immunity instructs adaptive immunity, we hypothesized that differences between populations in innate immune responses may represent a mechanistic link to variation in susceptibility to infection or response to vaccination. Determine whether differences in innate immune responses exist among infants from different continents of the world. We determined the innate cytokine response following pattern recognition receptor (PRR) stimulation of whole blood from 2-year-old infants across 4 continents (Africa, North America, South America, and Europe). We found that despite the many possible genetic and environmental exposure differences in infants across 4 continents, innate cytokine responses were similar for infants from North America, South America, and Europe. However, cells from South African infants secreted significantly lower levels of cytokines than did cells from infants from the 3 other sites, and did so following stimulation of extracellular and endosomal but not cytosolic PRRs. Substantial differences in innate cytokine responses to PRR stimulation exist among different populations of infants that could not have been predicted. Delineating the underlying mechanism(s) for these differences will not only aid in improving vaccine-mediated protection but possibly also provide clues for the susceptibility to infection in different regions of the world. Copyright © 2013 The Authors. Published by Mosby, Inc. All rights reserved.

The Architecture of Personality

ERIC Educational Resources Information Center

Cervone, Daniel

2004-01-01

This article presents a theoretical framework for analyzing psychological systems that contribute to the variability, consistency, and cross-situational coherence of personality functioning. In the proposed knowledge-and-appraisal personality architecture (KAPA), personality structures and processes are delineated by combining 2 principles:…

American Research on the Family and Socialization

ERIC Educational Resources Information Center

Clausen, John A.

1978-01-01

Comments on a number of trends in American research on the family as an instrument of socialization, stressing studies of contextual and structural features more than studies seeking to delineate relationships between specific parental practices and child behavior. (BR)

Rapid river classification using GIS-delineated functional process zones

EPA Science Inventory

Traditional classification of rivers does not take into consideration how rivers function within the ecosystem. Using factors such as hydrology and geomorphology that directly affect ecosystem structure and function, provides a means of classifying river systems into hydrogeomorp...

Delineation of gravel-bed clusters via factorial kriging

NASA Astrophysics Data System (ADS)

Wu, Fu-Chun; Wang, Chi-Kuei; Huang, Guo-Hao

2018-05-01

Gravel-bed clusters are the most prevalent microforms that affect local flows and sediment transport. A growing consensus is that the practice of cluster delineation should be based primarily on bed topography rather than grain sizes. Here we present a novel approach for cluster delineation using patch-scale high-resolution digital elevation models (DEMs). We use a geostatistical interpolation method, i.e., factorial kriging, to decompose the short- and long-range (grain- and microform-scale) DEMs. The required parameters are determined directly from the scales of the nested variograms. The short-range DEM exhibits a flat bed topography, yet individual grains are sharply outlined, making the short-range DEM a useful aid for grain segmentation. The long-range DEM exhibits a smoother topography than the original full DEM, yet groupings of particles emerge as small-scale bedforms, making the contour percentile levels of the long-range DEM a useful tool for cluster identification. Individual clusters are delineated using the segmented grains and identified clusters via a range of contour percentile levels. Our results reveal that the density and total area of delineated clusters decrease with increasing contour percentile level, while the mean grain size of clusters and average size of anchor clast (i.e., the largest particle in a cluster) increase with the contour percentile level. These results support the interpretation that larger particles group as clusters and protrude higher above the bed than other smaller grains. A striking feature of the delineated clusters is that anchor clasts are invariably greater than the D90 of the grain sizes even though a threshold anchor size was not adopted herein. The average areal fractal dimensions (Hausdorff-Besicovich dimensions of the projected areas) of individual clusters, however, demonstrate that clusters delineated with different contour percentile levels exhibit similar planform morphologies. Comparisons with a compilation of existing field data show consistency with the cluster properties documented in a wide variety of settings. This study thus points toward a promising, alternative DEM-based approach to characterizing sediment structures in gravel-bed rivers.

Geological map of parts of the state of Sao Paulo based on LANDSAT images. [Brazil

NASA Technical Reports Server (NTRS)

Dejususparada, N. (Principal Investigator); Amaral, G.; Liu, C. C.; Filho, R. A.

1979-01-01

The author has identified the following significant results. Interpretation of LANDSAT images revealed the subdivision of the Bauru formation into three distinct lithofacies. Delineation of structural features yielded new information on paleoenvironmental reconstitution and hydrogeology. Structural features and photogeological units were revealed in the precambrian basement at the eastern portion of the state.

An Army Force Structure for the Future

DTIC Science & Technology

1992-03-31

realization: deterring aggression; ensuring access to foreign markets , energy, mineral resources, the oceans, and space; maintaining stable regional...establish the optimum organisational mix for independent and highly flexible operational-level activity." 7 Two factors are driving this structural change...armored forces; and optimizing the force mix of the three. However, before describing the specific changes needed to shape the future Army, a delineation

Integration of multi-disciplinary geospatial data for delineating agroecosystem uniform management zones.

PubMed

Liu, Huanjun; Huffman, Ted; Liu, Jiangui; Li, Zhe; Daneshfar, Bahram; Zhang, Xinle

2015-01-01

Understanding agricultural ecosystems and their complex interactions with the environment is important for improving agricultural sustainability and environmental protection. Developing the necessary understanding requires approaches that integrate multi-source geospatial data and interdisciplinary relationships at different spatial scales. In order to identify and delineate landscape units representing relatively homogenous biophysical properties and eco-environmental functions at different spatial scales, a hierarchical system of uniform management zones (UMZ) is proposed. The UMZ hierarchy consists of seven levels of units at different spatial scales, namely site-specific, field, local, regional, country, continent, and globe. Relatively few studies have focused on the identification of the two middle levels of units in the hierarchy, namely the local UMZ (LUMZ) and the regional UMZ (RUMZ), which prevents true eco-environmental studies from being carried out across the full range of scales. This study presents a methodology to delineate LUMZ and RUMZ spatial units using land cover, soil, and remote sensing data. A set of objective criteria were defined and applied to evaluate the within-zone homogeneity and between-zone separation of the delineated zones. The approach was applied in a farming and forestry region in southeastern Ontario, Canada, and the methodology was shown to be objective, flexible, and applicable with commonly available spatial data. The hierarchical delineation of UMZs can be used as a tool to organize the spatial structure of agricultural landscapes, to understand spatial relationships between cropping practices and natural resources, and to target areas for application of specific environmental process models and place-based policy interventions.

Structural interpretation of the Ifal Basin in north-western Saudi Arabia from aeromagnetic data: hydrogeological and environmental implications

NASA Astrophysics Data System (ADS)

Elawadi, Eslam; Zaman, Haider; Batayneh, Awni; Mogren, Saad; Laboun, Abdalaziz; Ghrefat, Habes; Zumlot, Taisser

2013-09-01

The Ifal (Midyan) Basin is one of the well defined basins along the Red Sea coast, north-western Saudi Arabia. Location, geometry, thick sedimentary cover and structural framework qualify this basin for groundwater, oil and mineral occurrences. In spite of being studied by two airborne magnetic surveys during 1962 and 1983, structural interpretation of the area from a magnetic perspective, and its uses for hydrogeological and environmental investigations, has not been attempted. This work thus presents interpretation of the aeromagnetic data for basement depth estimation and tectonic framework delineation, which both have a role in controlling groundwater flow and accumulation in the Ifal Basin. A maximum depth of 3.5km is estimated for the basement surface by this study. In addition, several faulted and tilted blocks, perpendicularly dissected by NE-trending faults, are delineated within the structural framework of the study area. It is also observed that the studied basin is bounded by NW- and NE-trending faults. All these multi-directional faults/fracture systems in the Ifal Basin could be considered as conduits for groundwater accumulation, but with a possibility of environmental contamination from the surrounding soils and rock bodies.

Genetic analysis of Black Tiger shrimp (Penaeus monodon) across its natural distribution range reveals more recent colonization of Fiji and other South Pacific islands.

PubMed

Waqairatu, Salote S; Dierens, Leanne; Cowley, Jeff A; Dixon, Tom J; Johnson, Karyn N; Barnes, Andrew C; Li, Yutao

2012-08-01

The Black Tiger shrimp (Penaeus monodon) has a natural distribution range from East Africa to the South Pacific Islands. Although previous studies of Indo-Pacific P. monodon have found populations from the Indian Ocean and Australasia to differ genetically, their relatedness to South Pacific shrimp remains unknown. To address this, polymorphisms at eight shared microsatellite loci and haplotypes in a 418-bp mtDNA-CR (control region) sequence were examined across 682 P. monodon from locations spread widely across its natural range, including the South Pacific islands of Fiji, Palau, and Papua New Guinea (PNG). Observed microsatellite heterozygosities of 0.82-0.91, allele richness of 6.85-9.69, and significant mtDNA-CR haplotype variation indicated high levels of genetic diversity among the South Pacific shrimp. Analysis of microsatellite genotypes using a Bayesian STRUCTURE method segregated Indo-Pacific P. monodon into eight distinct clades, with Palau and PNG shrimp clustering among others from Southeast Asia and eastern Australia, respectively, and Fiji shrimp clustering as a distinct group. Phylogenetic analyses of mtDNA-CR haplotypes delineated shrimp into three groupings, with shrimp from Fiji again being distinct by sharing no haplotypes with other populations. Depending on regional location, the genetic structures and substructures identified from the genotyping and mtDNA-CR haplotype phylogeny could be explained by Metapopulation and/or Member-Vagrant type evolutionary processes. Neutrality tests of mutation-drift equilibrium and estimation of the time since population expansion supported a hypothesis that South Pacific P. monodon were colonized from Southeast Asia and eastern Australia during the Pleistocene period over 60,000 years ago when land bridges were more expansive and linked these regions more closely.

Genetic analysis of Black Tiger shrimp (Penaeus monodon) across its natural distribution range reveals more recent colonization of Fiji and other South Pacific islands

PubMed Central

Waqairatu, Salote S; Dierens, Leanne; Cowley, Jeff A; Dixon, Tom J; Johnson, Karyn N; Barnes, Andrew C; Li, Yutao

2012-01-01

The Black Tiger shrimp (Penaeus monodon) has a natural distribution range from East Africa to the South Pacific Islands. Although previous studies of Indo-Pacific P. monodon have found populations from the Indian Ocean and Australasia to differ genetically, their relatedness to South Pacific shrimp remains unknown. To address this, polymorphisms at eight shared microsatellite loci and haplotypes in a 418-bp mtDNA-CR (control region) sequence were examined across 682 P. monodon from locations spread widely across its natural range, including the South Pacific islands of Fiji, Palau, and Papua New Guinea (PNG). Observed microsatellite heterozygosities of 0.82–0.91, allele richness of 6.85–9.69, and significant mtDNA-CR haplotype variation indicated high levels of genetic diversity among the South Pacific shrimp. Analysis of microsatellite genotypes using a Bayesian STRUCTURE method segregated Indo-Pacific P. monodon into eight distinct clades, with Palau and PNG shrimp clustering among others from Southeast Asia and eastern Australia, respectively, and Fiji shrimp clustering as a distinct group. Phylogenetic analyses of mtDNA-CR haplotypes delineated shrimp into three groupings, with shrimp from Fiji again being distinct by sharing no haplotypes with other populations. Depending on regional location, the genetic structures and substructures identified from the genotyping and mtDNA-CR haplotype phylogeny could be explained by Metapopulation and/or Member–Vagrant type evolutionary processes. Neutrality tests of mutation-drift equilibrium and estimation of the time since population expansion supported a hypothesis that South Pacific P. monodon were colonized from Southeast Asia and eastern Australia during the Pleistocene period over 60,000 years ago when land bridges were more expansive and linked these regions more closely. PMID:22957205

Quantity, structure, and habitat selection of natural spawning reefs by walleyes in a north temperate lake: A multiscale analysis

USGS Publications Warehouse

Raabe, Joshua K.; Bozek, Michael A.

2012-01-01

Spawning habitat, the cornerstone of self-sustaining, naturally reproducing walleyeSander vitreus populations, has received limited quantitative research. Our goal was to quantitatively describe the structure and quantity of natural walleye spawning habitat and evaluate potential selection of habitat in Big Crooked Lake, Wisconsin. In 2004 and 2005, we located and delineated walleye egg deposition polygons through visual snorkel and scuba surveys. We also delineated recently deposited, adhesive egg patches daily along one spawning reef in 2005. To determine habitat selection, we quantified and compared spawning and lakewide available habitat at different scales. In both years, walleyes used similar spawning habitat, including three geomorphic types: linear shorelines, a point bar, and an island. Walleyes used only 14% of the entire lake shoreline and 39% of the shoreline comprised of gravel (6.4–76.0 mm), cobble (76.1–149.9 mm), or coarser substrates for spawning in 2005, indicating selection of specific spawning habitat. Lakewide, walleyes spawned close to shore (outer egg deposition polygon boundary mean distance = 2.7 m), in shallow water (outer egg deposition polygon boundary mean depth = 0.3 m), and over gravel substrate (percent coverage mean = 64.3) having low embeddedness (mean = 1.30). Our best nearshore (0–13-m) resource selection function predicted an increase in the relative probability of egg deposition with the increasing abundance of gravel, cobble, and rubble (150.0–303.9-mm) substrates and a decrease with increasing distance from shore and water depth (89.9% overall correct classification). Adhesive egg patches confirmed that walleyes actively chose nearshore, shallow-water, and coarse-substrate spawning habitat. The quantitative habitat information and predictive models will assist biologists in developing walleye spawning reef protection strategies and potentially aid in designing and evaluating artificial spawning reefs.

Comparative age and growth of common snook Centropomus undecimalis (Pisces: Centropomidae) from coastal and riverine areas in Southern Mexico.

PubMed

Perera-Garcia, Martha A; Mendoza-Carranza, Manuel; Contreras-Sánchez, Wilfrido; Ferrara, Allyse; Huerta-Ortiz, Maricela; Hernández-Gómez, Raúl E

2013-06-01

Common snook Centropomus unidecimalis is an important commercial and fishery species in Southern Mexico, however the high exploitation rates have resulted in a strong reduction of its abundances. Since, the information about its population structure is scarce, the objective of the present research was to determine and compare the age structure in four important fishery sites. For this, age and growth of common snook were determined from specimens collected monthly, from July 2006 to March 2008, from two coastal (Barra Bosque and Barra San Pedro) and two riverine (San Pedro and Tres Brazos) commercial fishery sites in Tabasco, Mexico. Age was determined using sectioned saggitae otoliths and data analyzed by von Bertalanffy and Levenberg-Marquardt among others. Estimated ages ranged from 2 to 17 years. Monthly patterns of marginal increment formation and the percentage of otoliths with opaque rings on the outer edge demonstrated that a single annulus was formed each year. The von Bertalanffy parameters were calculated for males and females using linear adjustment and the non-linear method of Levenberg-Marquardt. The von Bertalanffy growth equations were FLt = 109.21(1-e-0.2(t+0.57)) for Barra Bosque, FLt = 94.56(1-e-027(t+0.485)) for Barra San Pedro, FLt = 97.15(1-e 0.17(t + 1.32)) for San Pedro and FLt = 83.77(1-e-026(t + 0.49)) for Tres Brazos. According to (Hotelling's T2, p < 0.05) test growth was significantly greater for females than for males. Based on the Chen test, von Bertalanffy growth curves were different among the study sites (RSS, p < 0.05). Based on the observed differences in growth parameters among sampling sites (coastal and riverine environments) future research need to be conducted on migration and population genetics, in order to delineate the stock structure of this population and support management programs.

Comprehensive cellular‐resolution atlas of the adult human brain

PubMed Central

Royall, Joshua J.; Sunkin, Susan M.; Ng, Lydia; Facer, Benjamin A.C.; Lesnar, Phil; Guillozet‐Bongaarts, Angie; McMurray, Bergen; Szafer, Aaron; Dolbeare, Tim A.; Stevens, Allison; Tirrell, Lee; Benner, Thomas; Caldejon, Shiella; Dalley, Rachel A.; Dee, Nick; Lau, Christopher; Nyhus, Julie; Reding, Melissa; Riley, Zackery L.; Sandman, David; Shen, Elaine; van der Kouwe, Andre; Varjabedian, Ani; Write, Michelle; Zollei, Lilla; Dang, Chinh; Knowles, James A.; Koch, Christof; Phillips, John W.; Sestan, Nenad; Wohnoutka, Paul; Zielke, H. Ronald; Hohmann, John G.; Jones, Allan R.; Bernard, Amy; Hawrylycz, Michael J.; Hof, Patrick R.; Fischl, Bruce

2016-01-01

ABSTRACT Detailed anatomical understanding of the human brain is essential for unraveling its functional architecture, yet current reference atlases have major limitations such as lack of whole‐brain coverage, relatively low image resolution, and sparse structural annotation. We present the first digital human brain atlas to incorporate neuroimaging, high‐resolution histology, and chemoarchitecture across a complete adult female brain, consisting of magnetic resonance imaging (MRI), diffusion‐weighted imaging (DWI), and 1,356 large‐format cellular resolution (1 µm/pixel) Nissl and immunohistochemistry anatomical plates. The atlas is comprehensively annotated for 862 structures, including 117 white matter tracts and several novel cyto‐ and chemoarchitecturally defined structures, and these annotations were transferred onto the matching MRI dataset. Neocortical delineations were done for sulci, gyri, and modified Brodmann areas to link macroscopic anatomical and microscopic cytoarchitectural parcellations. Correlated neuroimaging and histological structural delineation allowed fine feature identification in MRI data and subsequent structural identification in MRI data from other brains. This interactive online digital atlas is integrated with existing Allen Institute for Brain Science gene expression atlases and is publicly accessible as a resource for the neuroscience community. J. Comp. Neurol. 524:3127–3481, 2016. © 2016 The Authors The Journal of Comparative Neurology Published by Wiley Periodicals, Inc. PMID:27418273

Stable isotope ratios and reforestation potential in Acacia koa populations on Hawai'i

Treesearch

Shaneka Lawson; Carrie Pike

2017-01-01

Stable carbon and nitrogen isotopes can be influenced by a multitude of factors including elevation, precipitation rate, season, and temperature. This work examined variability in foliar stable carbon (δ13C) and nitrogen (δ15N) isotope ratios of koa (Acacia koa) across 17 sites on Hawai'i Island, delineated by elevation and precipitation...

Using neutral, selected, and hitchhiker loci to assess connectivity of marine populations in the genomic era

PubMed Central

Gagnaire, Pierre-Alexandre; Broquet, Thomas; Aurelle, Didier; Viard, Frédérique; Souissi, Ahmed; Bonhomme, François; Arnaud-Haond, Sophie; Bierne, Nicolas

2015-01-01

Estimating the rate of exchange of individuals among populations is a central concern to evolutionary ecology and its applications to conservation and management. For instance, the efficiency of protected areas in sustaining locally endangered populations and ecosystems depends on reserve network connectivity. The population genetics theory offers a powerful framework for estimating dispersal distances and migration rates from molecular data. In the marine realm, however, decades of molecular studies have met limited success in inferring genetic connectivity, due to the frequent lack of spatial genetic structure in species exhibiting high fecundity and dispersal capabilities. This is especially true within biogeographic regions bounded by well-known hotspots of genetic differentiation. Here, we provide an overview of the current methods for estimating genetic connectivity using molecular markers and propose several directions for improving existing approaches using large population genomic datasets. We highlight several issues that limit the effectiveness of methods based on neutral markers when there is virtually no genetic differentiation among samples. We then focus on alternative methods based on markers influenced by selection. Although some of these methodologies are still underexplored, our aim was to stimulate new research to test how broadly they are applicable to nonmodel marine species. We argue that the increased ability to apply the concepts of cline analyses will improve dispersal inferences across physical and ecological barriers that reduce connectivity locally. We finally present how neutral markers hitchhiking with selected loci can also provide information about connectivity patterns within apparently well-mixed biogeographic regions. We contend that one of the most promising applications of population genomics is the use of outlier loci to delineate relevant conservation units and related eco-geographic features across which connectivity can be measured. PMID:26366195

Oblique along path toward structures at rear of parcel. Original ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Oblique along path toward structures at rear of parcel. Original skinny mosaic path along edge of structures was altered (delineation can be seen in concrete) path was widened with a newer mosaic to make access to the site safer. Structures (from right) edge of Round House (with "Spring Garden"), Pencil house, Shell House, School House, wood lattice is attached to chain-link fence along north (rear) property line. These structures were all damaged by the 1994 Northridge earthquake. Camera facing northeast. - Grandma Prisbrey's Bottle Village, 4595 Cochran Street, Simi Valley, Ventura County, CA

Urban Evolutionary Ecology and the Potential Benefits of Implementing Genomics.

PubMed

Schell, Christopher J

2018-02-14

Urban habitats are quickly becoming exceptional models to address adaptation under rapid environmental change, given the expansive temporal and spatial scales with which anthropogenic landscape conversion occurs. Urban ecologists in the last 10-15 years have done an extraordinary job of highlighting phenotypic patterns that correspond with urban living, as well as delineating urban population structure using traditional genetic markers. The underpinning genetic mechanisms that govern those phenotypic patterns, however, are less well established. Moreover, the power of traditional molecular studies is constrained by the number of markers being evaluated, which limits the potential to assess fine-scale population structure potentially common in urban areas. With the recent proliferation of low-cost, high-throughput sequencing methods, we can begin to address an emerging question in urban ecology: are species adapted to local optima within cities or are they expressing latent phenotypic plasticity? Here, I provide a comprehensive review of previous urban ecological studies, with special focus on the molecular ecology and phenotypic adjustments documented in urban terrestrial and amphibious fauna. I subsequently pinpoint areas in the literature that could benefit from a genomic investigation and briefly discuss the suitability of specific techniques in addressing eco-evolutionary questions within urban ecology. Though many challenges exist with implementing genomics into urban ecology, such studies provide an exceptional opportunity to advance our understanding of eco-evolutionary processes in metropolitan areas. © The American Genetic Association 2018. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

THE NEXT GENERATION OF VMT REDUCTION PROGRAMS

EPA Science Inventory

This research is structured to provide a clear delineation of factors that influence trip chaining, identify levels of flexibility in commuter travel, present a market segmentation of commuters in terms of their flexibility levels, and estimate the reach of current programs. ...

Genome-wide SNPs lead to strong signals of geographic structure and relatedness patterns in the major arbovirus vector, Aedes aegypti.

PubMed

Rašić, Gordana; Filipović, Igor; Weeks, Andrew R; Hoffmann, Ary A

2014-04-11

Genetic markers are widely used to understand the biology and population dynamics of disease vectors, but often markers are limited in the resolution they provide. In particular, the delineation of population structure, fine scale movement and patterns of relatedness are often obscured unless numerous markers are available. To address this issue in the major arbovirus vector, the yellow fever mosquito (Aedes aegypti), we used double digest Restriction-site Associated DNA (ddRAD) sequencing for the discovery of genome-wide single nucleotide polymorphisms (SNPs). We aimed to characterize the new SNP set and to test the resolution against previously described microsatellite markers in detecting broad and fine-scale genetic patterns in Ae. aegypti. We developed bioinformatics tools that support the customization of restriction enzyme-based protocols for SNP discovery. We showed that our approach for RAD library construction achieves unbiased genome representation that reflects true evolutionary processes. In Ae. aegypti samples from three continents we identified more than 18,000 putative SNPs. They were widely distributed across the three Ae. aegypti chromosomes, with 47.9% found in intergenic regions and 17.8% in exons of over 2,300 genes. Pattern of their imputed effects in ORFs and UTRs were consistent with those found in a recent transcriptome study. We demonstrated that individual mosquitoes from Indonesia, Australia, Vietnam and Brazil can be assigned with a very high degree of confidence to their region of origin using a large SNP panel. We also showed that familial relatedness of samples from a 0.4 km2 area could be confidently established with a subset of SNPs. Using a cost-effective customized RAD sequencing approach supported by our bioinformatics tools, we characterized over 18,000 SNPs in field samples of the dengue fever mosquito Ae. aegypti. The variants were annotated and positioned onto the three Ae. aegypti chromosomes. The new SNP set provided much greater resolution in detecting population structure and estimating fine-scale relatedness than a set of polymorphic microsatellites. RAD-based markers demonstrate great potential to advance our understanding of mosquito population processes, critical for implementing new control measures against this major disease vector.

The Green, Amber, Red Delineation of Risk and Need (GARDIAN) management system: a pragmatic approach to optimizing heart health from primary prevention to chronic disease management.

PubMed

Carrington, Melinda J; Kok, Simone; Jansen, Kiki; Stewart, Simon

2013-08-01

A sustained epidemic of cardiovascular disease and related risk factors is a global phenomenon contributing significantly to premature deaths and costly morbidity. Preventative strategies across the full continuum of life, from a population to individual perspective, are not optimally applied. This paper describes a simple and adaptable 'traffic-light' system we have developed to systematically perform individual risk and need delineation in order to 'titrate' the intensity and frequency of healthcare intervention in a cost-effective manner. The GARDIAN (Green Amber Red Delineation of Risk and Need) system is an individual assessment of risk and need that modulates the frequency and intensity of future healthcare intervention. Individual assessment of risk and need for ongoing intervention and support is determined with reference to three domains: (1) clinical stability, (2) gold-standard management, and (3) a broader, holistic assessment of individual circumstance. This can be applied from a primary prevention, secondary prevention, or chronic disease management perspective. Our experience with applying and validating GARDIAN to titrate healthcare resources according to need has been extensive to date, with >5000 individuals profiled in a host of clinical settings. A series of clinical randomized trials will determine the impact of the GARDIAN system on important indices of healthcare utilization and health status. The GARDIAN model to delineating risk and need for varied intensity of management shows strong potential to cost effectively improve health outcomes for both individuals at risk of heart disease and those with established heart disease.

Cytoarchitectonic identification and probabilistic mapping of two distinct areas within the anterior ventral bank of the human intraparietal sulcus

PubMed Central

Choi, Hi-Jae; Zilles, Karl; Mohlberg, Hartmut; Schleicher, Axel; Fink, Gereon R.; Armstrong, Este; Amunts, Katrin

2008-01-01

Anatomical studies in the macaque cortex and functional imaging studies in humans have demonstrated the existence of different cortical areas within the IntraParietal Sulcus (IPS). Such functional segregation, however, does not correlate with presently available architectonic maps of the human brain. This is particularly true for the classical Brodmann map, which is still widely used as an anatomical reference in functional imaging studies. The aim of this cytoarchitectonic mapping study was to use previously defined algorithms to determine whether consistent regions and borders can be found within the cortex of the anterior IPS in a population of ten postmortem human brains. Two areas, the human IntraParietal area 1 (hIP1) and the human IntraParietal area 2 (hIP2), were delineated in serial histological sections of the anterior, lateral bank of the human IPS. The region hIP1 is located posterior and medial to hIP2, and the former is always within the depths of the IPS. The latter, on the other hand, sometimes reaches the free surface of the superior parietal lobule. The delineations were registered to standard reference space, and probabilistic maps were calculated, thereby quantifying the intersubject variability in location and extent of both areas. In the future, they can be a tool in analyzing structure – function relationships and a basis for determining degrees of homology in the IPS among anthropoid primates. We conclude that the human intraparietal sulcus has a finer grained parcellation than shown in Brodmann’s map. PMID:16432904

Design considerations for composite fuselage structure of commercial transport aircraft

NASA Technical Reports Server (NTRS)

Davis, G. W.; Sakata, I. F.

1981-01-01

The structural, manufacturing, and service and environmental considerations that could impact the design of composite fuselage structure for commercial transport aircraft application were explored. The severity of these considerations was assessed and the principal design drivers delineated. Technical issues and potential problem areas which must be resolved before sufficient confidence is established to commit to composite materials were defined. The key issues considered are: definition of composite fuselage design specifications, damage tolerance, and crashworthiness.

Phylogenetics, phylogeography and population genetics of North American sea ducks (tribe: Mergini)

USGS Publications Warehouse

Talbot, Sandra L.; Sonsthagen, Sarah A.; Pearce, John M.; Scribner, Kim T.

2015-01-01

Many environments occupied by North American sea ducks are remote and difficult to access, and as a result, detailed information about life history characteristics that drive population dynamics within and across species is limited. Nevertheless, progress on this front during the past several decades has benefited by the application of genetic technologies, and for several species, these technologies have allowed for concomitant tracking of population trends and genetic diversity, delineation of populations, assessment of gene flow among metapopulations, and understanding of migratory connectivity between breeding and wintering grounds. This chapter provides an overview of phylogenetic, phylogeographic, and population genetics studies of North American sea duck species, many of which have sought to understand the major and minor genetic divisions within and among sea duck species, and most of which have been conducted with the understanding that the maintenance of genetic variation in wild sea duck populations is fundamental to the group’s long-term persistence.

Hypertext and Multimedia for Functional Enhancement of USARIEM Medical Handbooks and Biomedical Simulation Software.

DTIC Science & Technology

1994-12-01

complex Internet addresses. Hypertext and hypermedia documents have logical and physical structure (Shneiderman, 1993). The logical structure delineates...Rubra, Miliaria Profunda , Anhidrotic Heat Exhaustion, Heat Syncope, Heat Edema, Sunburn, and Heat Tetany. The user may return to the main document...military or scientific organizations via digital communications networks such as the Internet . Access clearance would first be obtained from the USARIEM

Visualizing geoelectric - Hydrogeological parameters of Fadak farm at Najaf Ashraf by using 2D spa

NASA Astrophysics Data System (ADS)

Al-Khafaji, Wadhah Mahmood Shakir; Al-Dabbagh, Hayder Abdul Zahra

2016-12-01

A geophysical survey achieved to produce an electrical resistivity grid data of 23 Schlumberger Vertical Electrical Sounding (VES) points distributed across the area of Fadak farm at Najaf Ashraf province in Iraq. The current research deals with the application of six interpolation methods used to delineate subsurface groundwater aquifer properties. One example of such features is the delineation of high and low groundwater hydraulic conductivity (K). Such methods could be useful in predicting high (K) zones and predicting groundwater flowing directions within the studied aquifer. Interpolation methods were helpful in predicting some aquifer hydrogeological and structural characteristics. The results produced some important conclusions for any future groundwater investment.

SU-E-J-134: Optimizing Technical Parameters for Using Atlas Based Automatic Segmentation for Evaluation of Contour Accuracy Experience with Cardiac Structures From NRG Oncology/RTOG 0617

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yu, J; Gong, Y; Bar-Ad, V

Purpose: Accurate contour delineation is crucial for radiotherapy. Atlas based automatic segmentation tools can be used to increase the efficiency of contour accuracy evaluation. This study aims to optimize technical parameters utilized in the tool by exploring the impact of library size and atlas number on the accuracy of cardiac contour evaluation. Methods: Patient CT DICOMs from RTOG 0617 were used for this study. Five experienced physicians delineated the cardiac structures including pericardium, atria and ventricles following an atlas guideline. The consistency of cardiac structured delineation using the atlas guideline was verified by a study with four observers and seventeenmore » patients. The CT and cardiac structure DICOM files were then used for the ABAS technique.To study the impact of library size (LS) and atlas number (AN) on automatic contour accuracy, automatic contours were generated with varied technique parameters for five randomly selected patients. Three LS (20, 60, and 100) were studied using commercially available software. The AN was four, recommended by the manufacturer. Using the manual contour as the gold standard, Dice Similarity Coefficient (DSC) was calculated between the manual and automatic contours. Five-patient averaged DSCs were calculated for comparison for each cardiac structure.In order to study the impact of AN, the LS was set 100, and AN was tested from one to five. The five-patient averaged DSCs were also calculated for each cardiac structure. Results: DSC values are highest when LS is 100 and AN is four. The DSC is 0.90±0.02 for pericardium, 0.75±0.06 for atria, and 0.86±0.02 for ventricles. Conclusion: By comparing DSC values, the combination AN=4 and LS=100 gives the best performance. This project was supported by NCI grants U24CA12014, U24CA180803, U10CA180868, U10CA180822, PA CURE grant and Bristol-Myers Squibb and Eli Lilly.« less

Combining criteria for delineating lahar- and flash-flood-prone hazard and risk zones for the city of Arequipa, Peru

NASA Astrophysics Data System (ADS)

Thouret, J.-C.; Enjolras, G.; Martelli, K.; Santoni, O.; Luque, J. A.; Nagata, M.; Arguedas, A.; Macedo, L.

2013-02-01

Arequipa, the second largest city in Peru, is exposed to many natural hazards, most notably earthquakes, volcanic eruptions, landslides, lahars (volcanic debris flows), and flash floods. Of these, lahars and flash floods, triggered by occasional torrential rainfall, pose the most frequently occurring hazards that can affect the city and its environs, in particular the areas containing low-income neighbourhoods. This paper presents and discusses criteria for delineating areas prone to flash flood and lahar hazards, which are localized along the usually dry (except for the rainy season) ravines and channels of the Río Chili and its tributaries that dissect the city. Our risk-evaluation study is based mostly on field surveys and mapping, but we also took into account quality and structural integrity of buildings, available socio-economic data, and information gained from interviews with risk-managers officials. In our evaluation of the vulnerability of various parts of the city, in addition to geological and physical parameters, we also took into account selected socio-economic parameters, such as the educational and poverty level of the population, unemployment figures, and population density. In addition, we utilized a criterion of the "isolation factor", based on distances to access emergency resources (hospitals, shelters or safety areas, and water) in each city block. By combining the hazard, vulnerability and exposure criteria, we produced detailed risk-zone maps at the city-block scale, covering the whole city of Arequipa and adjacent suburbs. Not surprisingly, these maps show that the areas at high risk coincide with blocks or districts with populations at low socio-economic levels. Inhabitants at greatest risk are the poor recent immigrants from rural areas who live in unauthorized settlements in the outskirts of the city in the upper parts of the valleys. Such settlements are highly exposed to natural hazards and have little access to vital resources. Our study provides good rationale for the risk zoning of the city, which in turn may be used as an educational tool for better understanding the potential effects of natural hazards and the exposure of the population residing in and around Arequipa. We hope that our work and the risk-zonation maps will provide the impetus and basis for risk-management authorities of the Municipality and the regional government of Arequipa to enforce existing regulations in building in hazardous zones and to adopt an effective long-term strategy to reduce risks from lahar, flash flood, and other natural hazards.

The Electric Company Writers' Notebook.

ERIC Educational Resources Information Center

Children's Television Workshop, New York, NY.

This handbook outlines the curriculum objectives for the children's television program, "The Electric Company." The first portion of the text delineates strategies for teaching symbol/sound analysis, including units on blends, letter groups, and word structure. A second section addresses strategies for reading for meaning, including…

Governing Board Manual. Revised June, 1999.

ERIC Educational Resources Information Center

Palomar Community Coll. District, San Marcos, CA.

This document details the governance structure of Palomar Community College. The plan attempts to: (1) provide appropriate representation for each constituent group; (2) delineate committee responsibilities and reporting relationships; (3) establish the appropriate number and size of committees; (4) ensure that operational matters are handled…

Otolith chemistry discriminates natal signatures of yellowfin tuna (Thunnus albacares) in the Atlantic Ocean

NASA Astrophysics Data System (ADS)

Kitchens, L. L.; Rooker, J. R.

2016-02-01

In this study, the otolith chemistry of young-of-the-year (YOY) yellowfin tuna (Thunnus albacares) was examined to determine whether chemical signatures are distinct across different putative spawning areas in the Atlantic Ocean. Yellowfin tuna is a highly migratory species that is currently managed as a single panmictic stock in the Atlantic Ocean; however, uncertainty remains regarding the population structure of yellowfin in this region, particularly concerning the degree of mixing between spawning populations. Analysis of naturally occurring chemical tracers in otoliths provides a valuable means to reconstruct a fish's environmental history and is thus a promising approach for delineating stock structure of Atlantic yellowfin tuna. YOY yellowfin tuna otoliths were collected from 5 locations in the Atlantic Ocean (Gulf of Mexico, SE Caribbean, Brazil, Cape Verde, and Gulf of Guinea) from 2013-2015 and trace element (Li, Mg, Mn, Co, Cu, Sr, Zn, and Ba) and stable isotope (δ13C and δ18O) analyses were conducted to investigate regional variation in otolith chemical composition. Results show significant differences among nursery areas in both trace element (MANOVA, p<0.001) and δ13C and δ18O signatures (ANOVA, p=0.017 and p=0.001, respectively). Particularly high spatial separation was observed based on eastern Atlantic (Gulf of Guinea + Cape Verde) and western Atlantic (Gulf of Mexico + Brazil + Martinique) nursery areas, indicating the approach has promise for distinguishing migrants displaying trans-ocean movement. These chemical signatures will be used to assign adult yellowfin tuna to their nursery of origin, ultimately providing an improved understanding of the stock structure and movement of yellowfin tuna in the Atlantic Ocean.

Computation of a high-resolution MRI 3D stereotaxic atlas of the sheep brain.

PubMed

Ella, Arsène; Delgadillo, José A; Chemineau, Philippe; Keller, Matthieu

2017-02-15

The sheep model was first used in the fields of animal reproduction and veterinary sciences and then was utilized in fundamental and preclinical studies. For more than a decade, magnetic resonance (MR) studies performed on this model have been increasingly reported, especially in the field of neuroscience. To contribute to MR translational neuroscience research, a brain template and an atlas are necessary. We have recently generated the first complete T1-weighted (T1W) and T2W MR population average images (or templates) of in vivo sheep brains. In this study, we 1) defined a 3D stereotaxic coordinate system for previously established in vivo population average templates; 2) used deformation fields obtained during optimized nonlinear registrations to compute nonlinear tissues or prior probability maps (nlTPMs) of cerebrospinal fluid (CSF), gray matter (GM), and white matter (WM) tissues; 3) delineated 25 external and 28 internal sheep brain structures by segmenting both templates and nlTPMs; and 4) annotated and labeled these structures using an existing histological atlas. We built a quality high-resolution 3D atlas of average in vivo sheep brains linked to a reference stereotaxic space. The atlas and nlTPMs, associated with previously computed T1W and T2W in vivo sheep brain templates and nlTPMs, provide a complete set of imaging space that are able to be imported into other imaging software programs and could be used as standardized tools for neuroimaging studies or other neuroscience methods, such as image registration, image segmentation, identification of brain structures, implementation of recording devices, or neuronavigation. J. Comp. Neurol. 525:676-692, 2017. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

Tree crown mapping in managed woodlands (parklands) of semi-arid West Africa using WorldView-2 imagery and geographic object based image analysis.

PubMed

Karlson, Martin; Reese, Heather; Ostwald, Madelene

2014-11-28

Detailed information on tree cover structure is critical for research and monitoring programs targeting African woodlands, including agroforestry parklands. High spatial resolution satellite imagery represents a potentially effective alternative to field-based surveys, but requires the development of accurate methods to automate information extraction. This study presents a method for tree crown mapping based on Geographic Object Based Image Analysis (GEOBIA) that use spectral and geometric information to detect and delineate individual tree crowns and crown clusters. The method was implemented on a WorldView-2 image acquired over the parklands of Saponé, Burkina Faso, and rigorously evaluated against field reference data. The overall detection rate was 85.4% for individual tree crowns and crown clusters, with lower accuracies in areas with high tree density and dense understory vegetation. The overall delineation error (expressed as the difference between area of delineated object and crown area measured in the field) was 45.6% for individual tree crowns and 61.5% for crown clusters. Delineation accuracies were higher for medium (35-100 m(2)) and large (≥100 m(2)) trees compared to small (<35 m(2)) trees. The results indicate potential of GEOBIA and WorldView-2 imagery for tree crown mapping in parkland landscapes and similar woodland areas.

Tree Crown Mapping in Managed Woodlands (Parklands) of Semi-Arid West Africa Using WorldView-2 Imagery and Geographic Object Based Image Analysis

PubMed Central

Karlson, Martin; Reese, Heather; Ostwald, Madelene

2014-01-01

Detailed information on tree cover structure is critical for research and monitoring programs targeting African woodlands, including agroforestry parklands. High spatial resolution satellite imagery represents a potentially effective alternative to field-based surveys, but requires the development of accurate methods to automate information extraction. This study presents a method for tree crown mapping based on Geographic Object Based Image Analysis (GEOBIA) that use spectral and geometric information to detect and delineate individual tree crowns and crown clusters. The method was implemented on a WorldView-2 image acquired over the parklands of Saponé, Burkina Faso, and rigorously evaluated against field reference data. The overall detection rate was 85.4% for individual tree crowns and crown clusters, with lower accuracies in areas with high tree density and dense understory vegetation. The overall delineation error (expressed as the difference between area of delineated object and crown area measured in the field) was 45.6% for individual tree crowns and 61.5% for crown clusters. Delineation accuracies were higher for medium (35–100 m2) and large (≥100 m2) trees compared to small (<35 m2) trees. The results indicate potential of GEOBIA and WorldView-2 imagery for tree crown mapping in parkland landscapes and similar woodland areas. PMID:25460815

Quantitative and qualitative comparison of MR imaging of the temporomandibular joint at 1.5 and 3.0 T using an optimized high-resolution protocol

PubMed Central

Spinner, Georg; Wyss, Michael; Erni, Stefan; Ettlin, Dominik A; Nanz, Daniel; Ulbrich, Erika J; Gallo, Luigi M; Andreisek, Gustav

2016-01-01

Objectives: To quantitatively and qualitatively compare MRI of the temporomandibular joint (TMJ) using an optimized high-resolution protocol at 3.0 T and a clinical standard protocol at 1.5 T. Methods: A phantom and 12 asymptomatic volunteers were MR imaged using a 2-channel surface coil (standard TMJ coil) at 1.5 and 3.0 T (Philips Achieva and Philips Ingenia, respectively; Philips Healthcare, Best, Netherlands). Imaging protocol consisted of coronal and oblique sagittal proton density-weighted turbo spin echo sequences. For quantitative evaluation, a spherical phantom was imaged. Signal-to-noise ratio (SNR) maps were calculated on a voxelwise basis. For qualitative evaluation, all volunteers underwent MRI of the TMJ with the jaw in closed position. Two readers independently assessed visibility and delineation of anatomical structures of the TMJ and overall image quality on a 5-point Likert scale. Quantitative and qualitative measurements were compared between field strengths. Results: The quantitative analysis showed similar SNR for the high-resolution protocol at 3.0 T compared with the clinical protocol at 1.5 T. The qualitative analysis showed significantly better visibility and delineation of clinically relevant anatomical structures of the TMJ, including the TMJ disc and pterygoid muscle as well as better overall image quality at 3.0 T than at 1.5 T. Conclusions: The presented results indicate that expected gains in SNR at 3.0 T can be used to increase the spatial resolution when imaging the TMJ, which translates into increased visibility and delineation of anatomical structures of the TMJ. Therefore, imaging at 3.0 T should be preferred over 1.5 T for imaging the TMJ. PMID:26371077

Quantitative and qualitative comparison of MR imaging of the temporomandibular joint at 1.5 and 3.0 T using an optimized high-resolution protocol.

PubMed

Manoliu, Andrei; Spinner, Georg; Wyss, Michael; Erni, Stefan; Ettlin, Dominik A; Nanz, Daniel; Ulbrich, Erika J; Gallo, Luigi M; Andreisek, Gustav

2016-01-01

To quantitatively and qualitatively compare MRI of the temporomandibular joint (TMJ) using an optimized high-resolution protocol at 3.0 T and a clinical standard protocol at 1.5 T. A phantom and 12 asymptomatic volunteers were MR imaged using a 2-channel surface coil (standard TMJ coil) at 1.5 and 3.0 T (Philips Achieva and Philips Ingenia, respectively; Philips Healthcare, Best, Netherlands). Imaging protocol consisted of coronal and oblique sagittal proton density-weighted turbo spin echo sequences. For quantitative evaluation, a spherical phantom was imaged. Signal-to-noise ratio (SNR) maps were calculated on a voxelwise basis. For qualitative evaluation, all volunteers underwent MRI of the TMJ with the jaw in closed position. Two readers independently assessed visibility and delineation of anatomical structures of the TMJ and overall image quality on a 5-point Likert scale. Quantitative and qualitative measurements were compared between field strengths. The quantitative analysis showed similar SNR for the high-resolution protocol at 3.0 T compared with the clinical protocol at 1.5 T. The qualitative analysis showed significantly better visibility and delineation of clinically relevant anatomical structures of the TMJ, including the TMJ disc and pterygoid muscle as well as better overall image quality at 3.0 T than at 1.5 T. The presented results indicate that expected gains in SNR at 3.0 T can be used to increase the spatial resolution when imaging the TMJ, which translates into increased visibility and delineation of anatomical structures of the TMJ. Therefore, imaging at 3.0 T should be preferred over 1.5 T for imaging the TMJ.

Mapping subtrappean sediments and delineating structure with the aid of heliborne time domain electromagnetics: Case study from Kaladgi Basin, Karnataka

NASA Astrophysics Data System (ADS)

Sridhar, M.; Markandeyulu, A.; Chaturvedi, A. K.

2017-01-01

Mapping of subtrappean sediments is a complex geological problem attempted by many interpreters applying different geophysical techniques. Variations in thickness and resistivity of traps and underlying sediments, respectively, results in considerable uncertainty in the interpretation of geophysical data. It is proposed that the transient electromagnetic technique is an effective geophysical tool for delineation of the sub-trappean sediments, due to marked resistivity contrast between the Deccan trap, and underlying sediments and/or basement. The northern margin of the Kaladgi basin is covered under trap. A heliborne time domain electromagnetic survey was conducted to demarcate the basin extent and map the sub-trappean sediments. Conductivity depth transformations were used to map the interface between conductive trap and resistive 'basement'. Two resistivity contrast boundaries are picked: the first corresponds to the bottom of the shallow conductive unit interpreted as the base of the Deccan Volcanics and the second - picked at the base of a deeper subsurface conductive zone - is interpreted as the weathered paleo-surface of the crystalline basement. This second boundary can only be seen in areas where the volcanics are thin or absent, suggesting that the volcanics are masking the EM signal preventing deeper penetration. An interesting feature, which shows prominently in the EM data but less clearly imaged in the magnetic data, is observed in the vicinity of Mudhol. The surface geology interpreted from satellite imagery show Deccan trap cover around Mudhol. Modelling of TDEM data suggest the presence of synclinal basin structure. The depth of penetration of the heliborne TDEM data is estimated to be approximately 350 m for the study area. This suggests that heliborne TDEM could penetrate significant thicknesses of conductive Deccan trap cover to delineate structure below in the Bagalkot Group.

Combat-related, chronic posttraumatic stress disorder: implications for group-therapy intervention.

PubMed

Makler, S; Sigal, M; Gelkopf, M; Kochba, B B; Horeb, E

1990-07-01

The patient with combat-related chronic Posttraumatic Stress Disorder suffers from a wide spectrum of maladaptive behaviors. This paper delineates the work that has been done with such a population in group therapy. The plan that is proposed takes into account three interrelated sets of factors: factors important for creating an effective working relation; curative factors; and particular themes. Each of these factors is analyzed in the light of the particularities of group work with such a population. Each of the points discussed is based upon the relevant literature, upon the experience of the therapist, and illustrated with examples.

Head Start Children, Families, and Programs: Present and Past Data from FACES. OPRE Report 2011-33a

ERIC Educational Resources Information Center

Hulsey, Lara Kristin; Aikens, Nikki; Kopack, Ashley; West, Jerry; Moiduddin, Emily; Tarullo, Louisa

2011-01-01

This report provides a portrait of children entering Head Start for the first time in fall 2009, as well as of their family backgrounds and the classrooms and programs that serve them. The report also offers comparisons across the past decade of the Head Start program to delineate trends and changes in the population served and the services…

An Assessment of the Nature and Extent of Community and Employee Fitness Programs and Levels of Participation. Final Report. Report No. 9.

ERIC Educational Resources Information Center

Bozzo, Robert; And Others

This report presents an assessment and comparison of the nature and extent of general population, employee, and school fitness programs. Chapter I provides an overview of the research effort and the research questions developed as a framework for delineating issues to be examined. Chapter II identifies the generic approach used to examine the…

Marine parasites as biological tags in South American Atlantic waters, current status and perspectives.

PubMed

Cantatore, D M P; Timi, J T

2015-01-01

Many marine fisheries in South American Atlantic coasts (SAAC) are threatened by overfishing and under serious risk of collapsing. The SAAC comprises a diversity of environments, possesses a complex oceanography and harbours a vast biodiversity that provide an enormous potential for using parasites as biological tags for fish stock delineation, a prerequisite for the implementation of control and management plans. Here, their use in the SAAC is reviewed. Main evidence is derived from northern Argentine waters, where fish parasite assemblages are dominated by larval helminth species that share a low specificity, long persistence and trophic transmission, parasitizing almost indiscriminately all available fish species. The advantages and constraints of such a combination of characteristics are analysed and recommendations are given for future research. Shifting the focus from fish/parasite populations to communities allows expanding the concept of biological tags from local to regional scales, providing essential information to delineate ecosystem boundaries for host communities. This new concept arose as a powerful tool to help the implementation of ecosystem-based approaches to fisheries management, the new paradigm for fisheries science. Holistic approaches, including parasites as biological tags for stock delineation will render valuable information to help insure fisheries and marine ecosystems against further depletion and collapse.

Electron tomography and nano-diffraction enabling the investigation of individual magnetic nanoparticles inside fibers of MR visible implants

NASA Astrophysics Data System (ADS)

Slabu, I.; Wirch, N.; Caumanns, T.; Theissmann, R.; Krüger, M.; Schmitz-Rode, T.; Weirich, T. E.

2017-08-01

Superparamagnetic iron oxide nanoparticles (SPIONPs) incorporated into the base material of implants are used as contrast agents in magnetic resonance imaging for the delineation of the implants from the surrounding tissue. However, the delineation quality is strongly related to the structural characteristics of the incorporated SPIONPs and their interparticle interaction as well as their interaction with the polymer matrix of the implant. Consequently, a profound knowledge of the formation of aggregates inside the polymer matrix, which are responsible for strong interparticle interactions, and of their structural characteristics, is required for controlling the magnetic resonance image quality of the implants. In this work, transmission electron microscopy methods such as electron tomography and nano-electron diffraction were used to depict SPIONP aggregates inside the melt-spin polyvinylidene fluoride fibers used for the assembly of implants and to determine the crystal structure of individual nanocrystals inside these aggregates, respectively. Using these techniques it was possible for the first time to characterize the aggregates inside the fibers of implants and to validate the magnetization measurements that have been previously used to assess the interaction phenomena inside the fibers of implants. With electron tomography, inhomogeneously sized distributed aggregates were delineated and 3D models of these aggregates were constructed. Furthermore, the distribution of the aggregates inside the fibers was verified by means of magnetic force microscopy. With nano-diffraction measurements, the SPIONP crystal structure inside the fibers of the implant could not be clearly assigned to that of magnetite (Fe3O4) or maghemite (γ-Fe2O3). Therefore, additional electron energy loss spectroscopy measurements were performed, which revealed the presence of both phases of Fe3O4 and γ-Fe2O3, probably caused by oxidation processes during the manufacture of the fibers by melt-spinning.

GENETICALLY MODIFIED FOODS: TECHNOLOGICAL BREAKTHROUGH OR ECOLOGICAL NIGHMARE?

EPA Science Inventory

Fifty years ago, Wastson and Crick described the structure of DNA, setting the stage for the past decade's biotechnology revolution. Scientists have now broken the code of the entire human genome, and delineated the function of multiple genes; similar strides are being taken with...

Retheorizing Doctoral Supervision as Professional Work

ERIC Educational Resources Information Center

Halse, Christine; Malfroy, Janne

2010-01-01

A competitive higher education environment marked by increased accountability and quality assurance measures for doctoral study, including the structured training of doctoral supervisors, has highlighted the need to clearly articulate and delineate the work of supervising doctoral students. This article responds to this imperative by examining the…

Polymer Nomenclature--or What's in a Name?

ERIC Educational Resources Information Center

Carraher, Charles, E., Jr.; And Others

1987-01-01

Discusses the diversity of names used for various types of polymeric materials. Concentrates on the naming of linear organic polymers. Delineates these polymers by discussing common names, source-based names, characteristic group names, and structure-based names. Introduces the specifications of tacticity and geometric isomerism. (TW)

Structure of the ACF7 EF-Hand-GAR Module and Delineation of Microtubule Binding Determinants.

PubMed

Lane, Thomas R; Fuchs, Elaine; Slep, Kevin C

2017-07-05

Spectraplakins are large molecules that cross-link F-actin and microtubules (MTs). Mutations in spectraplakins yield defective cell polarization, aberrant focal adhesion dynamics, and dystonia. We present the 2.8 Å crystal structure of the hACF7 EF1-EF2-GAR MT-binding module and delineate the GAR residues critical for MT binding. The EF1-EF2 and GAR domains are autonomous domains connected by a flexible linker. The EF1-EF2 domain is an EFβ-scaffold with two bound Ca 2+ ions that straddle an N-terminal α helix. The GAR domain has a unique α/β sandwich fold that coordinates Zn 2+ . While the EF1-EF2 domain is not sufficient for MT binding, the GAR domain is and likely enhances EF1-EF2-MT engagement. Residues in a conserved basic patch, distal to the GAR domain's Zn 2+ -binding site, mediate MT binding. Copyright © 2017 Elsevier Ltd. All rights reserved.

From Brain Maps to Cognitive Ontologies: Informatics and the Search for Mental Structure.

PubMed

Poldrack, Russell A; Yarkoni, Tal

2016-01-01

A major goal of cognitive neuroscience is to delineate how brain systems give rise to mental function. Here we review the increasingly large role informatics-driven approaches are playing in such efforts. We begin by reviewing a number of challenges conventional neuroimaging approaches face in trying to delineate brain-cognition mappings--for example, the difficulty in establishing the specificity of postulated associations. Next, we demonstrate how these limitations can potentially be overcome using complementary approaches that emphasize large-scale analysis--including meta-analytic methods that synthesize hundreds or thousands of studies at a time; latent-variable approaches that seek to extract structure from data in a bottom-up manner; and predictive modeling approaches capable of quantitatively inferring mental states from patterns of brain activity. We highlight the underappreciated but critical role for formal cognitive ontologies in helping to clarify, refine, and test theories of brain and cognitive function. Finally, we conclude with a speculative discussion of what future informatics developments may hold for cognitive neuroscience.

From brain maps to cognitive ontologies: informatics and the search for mental structure

PubMed Central

Poldrack, Russell A.; Yarkoni, Tal

2015-01-01

A major goal of cognitive neuroscience is to delineate how brain systems give rise to mental function. Here we review the increasingly large role informatics-driven approaches are playing in such efforts. We begin by reviewing a number of challenges conventional neuroimaging approaches face in trying to delineate brain-cognition mappings—for example, the difficulty in establishing the specificity of postulated associations. Next, we demonstrate how these limitations can potentially be overcome using complementary approaches that emphasize large-scale analysis—including meta-analytic methods that synthesize hundreds or thousands of studies at a time; latent-variable approaches that seek to extract structure from data in a bottom-up manner; and predictive modeling approaches capable of quantitatively inferring mental states from patterns of brain activity. We highlight the underappreciated but critical role for formal cognitive ontologies in helping to clarify, refine, and test theories of brain and cognitive function. Finally, we conclude with a speculative discussion of what future informatics developments may hold for cognitive neuroscience. PMID:26393866

Stochastic Community Assembly: Does It Matter in Microbial Ecology?

PubMed

Zhou, Jizhong; Ning, Daliang

2017-12-01

Understanding the mechanisms controlling community diversity, functions, succession, and biogeography is a central, but poorly understood, topic in ecology, particularly in microbial ecology. Although stochastic processes are believed to play nonnegligible roles in shaping community structure, their importance relative to deterministic processes is hotly debated. The importance of ecological stochasticity in shaping microbial community structure is far less appreciated. Some of the main reasons for such heavy debates are the difficulty in defining stochasticity and the diverse methods used for delineating stochasticity. Here, we provide a critical review and synthesis of data from the most recent studies on stochastic community assembly in microbial ecology. We then describe both stochastic and deterministic components embedded in various ecological processes, including selection, dispersal, diversification, and drift. We also describe different approaches for inferring stochasticity from observational diversity patterns and highlight experimental approaches for delineating ecological stochasticity in microbial communities. In addition, we highlight research challenges, gaps, and future directions for microbial community assembly research. Copyright © 2017 American Society for Microbiology.

Solid-State (13)C NMR Delineates the Architectural Design of Biopolymers in Native and Genetically Altered Tomato Fruit Cuticles.

PubMed

Chatterjee, Subhasish; Matas, Antonio J; Isaacson, Tal; Kehlet, Cindie; Rose, Jocelyn K C; Stark, Ruth E

2016-01-11

Plant cuticles on outer fruit and leaf surfaces are natural macromolecular composites of waxes and polyesters that ensure mechanical integrity and mitigate environmental challenges. They also provide renewable raw materials for cosmetics, packaging, and coatings. To delineate the structural framework and flexibility underlying the versatile functions of cutin biopolymers associated with polysaccharide-rich cell-wall matrices, solid-state NMR spectra and spin relaxation times were measured in a tomato fruit model system, including different developmental stages and surface phenotypes. The hydrophilic-hydrophobic balance of the cutin ensures compatibility with the underlying polysaccharide cell walls; the hydroxy fatty acid structures of outer epidermal cutin also support deposition of hydrophobic waxes and aromatic moieties while promoting the formation of cell-wall cross-links that rigidify and strengthen the cuticle composite during fruit development. Fruit cutin-deficient tomato mutants with compromised microbial resistance exhibit less efficient local and collective biopolymer motions, stiffening their cuticular surfaces and increasing their susceptibility to fracture.

Two Sides of the Same Coin: Cannabis Dependence and Mental Health Problems in Help-Seeking Adolescent and Young Adult Outpatients

ERIC Educational Resources Information Center

Norberg, Melissa M.; Battisti, Robert A.; Copeland, Jan; Hermens, Daniel F.; Hickie, Ian B.

2012-01-01

The aim of the current study was to delineate the psychiatric profile of cannabis dependent young people (14-29 years old) with mental health problems (N = 36) seeking treatment via a research study. To do so, the Structured Clinical Interview for DSM-IV-TR Axis I Disorders and the Structured Clinical Interview for DSM-IV Childhood Diagnoses were…

Use of metallic glasses for fabrication of structures with submicron dimensions

DOEpatents

Wiley, John D.; Perepezko, John H.

1986-01-01

Patterned structures of submicron dimension formed of supported or unsupported amorphous metals having submicron feature sizes characterized by etching behavior sufficient to allow delineation of sharp edges and smooth flat flanks, resistance to time-dependent dimensional changes caused by creep, flow, in-diffusion of unwanted impurities, out-diffusion of constituent atoms, void formation, grain growth or phase separation and resistance to phase transformations or compound formation.

Application of LANDSAT satellite imagery for iron ore prospecting in the western desert of Egypt

NASA Technical Reports Server (NTRS)

Elshazly, E. M.; Abdel-Hady, M. A.; Elghawaby, M. A.; Khawasik, S. M. (Principal Investigator)

1977-01-01

The author has identified the following significant results. The delineation of the geological units and geological structures through image interpretation, corroborated by field observations and structural analysis, led to the discovery of new iron ore deposits. A new locality for iron ore deposition, namely Gebel Qalamun, was discovered, as well as new occurrences within the already known iron ore region of Bahariya Oasis.

Matching spatial with ontological brain regions using Java tools for visualization, database access, and integrated data analysis.

PubMed

Bezgin, Gleb; Reid, Andrew T; Schubert, Dirk; Kötter, Rolf

2009-01-01

Brain atlases are widely used in experimental neuroscience as tools for locating and targeting specific brain structures. Delineated structures in a given atlas, however, are often difficult to interpret and to interface with database systems that supply additional information using hierarchically organized vocabularies (ontologies). Here we discuss the concept of volume-to-ontology mapping in the context of macroscopical brain structures. We present Java tools with which we have implemented this concept for retrieval of mapping and connectivity data on the macaque brain from the CoCoMac database in connection with an electronic version of "The Rhesus Monkey Brain in Stereotaxic Coordinates" authored by George Paxinos and colleagues. The software, including our manually drawn monkey brain template, can be downloaded freely under the GNU General Public License. It adds value to the printed atlas and has a wider (neuro-)informatics application since it can read appropriately annotated data from delineated sections of other species and organs, and turn them into 3D registered stacks. The tools provide additional features, including visualization and analysis of connectivity data, volume and centre-of-mass estimates, and graphical manipulation of entire structures, which are potentially useful for a range of research and teaching applications.

The Case for Adopting the "Species Complex" Nomenclature for the Etiologic Agents of Cryptococcosis.

PubMed

Kwon-Chung, Kyung J; Bennett, John E; Wickes, Brian L; Meyer, Wieland; Cuomo, Christina A; Wollenburg, Kurt R; Bicanic, Tihana A; Castañeda, Elizabeth; Chang, Yun C; Chen, Jianghan; Cogliati, Massimo; Dromer, Françoise; Ellis, David; Filler, Scott G; Fisher, Matthew C; Harrison, Thomas S; Holland, Steven M; Kohno, Shigeru; Kronstad, James W; Lazera, Marcia; Levitz, Stuart M; Lionakis, Michail S; May, Robin C; Ngamskulrongroj, Popchai; Pappas, Peter G; Perfect, John R; Rickerts, Volker; Sorrell, Tania C; Walsh, Thomas J; Williamson, Peter R; Xu, Jianping; Zelazny, Adrian M; Casadevall, Arturo

2017-01-01

Cryptococcosis is a potentially lethal disease of humans/animals caused by Cryptococcus neoformans and Cryptococcus gattii . Distinction between the two species is based on phenotypic and genotypic characteristics. Recently, it was proposed that C. neoformans be divided into two species and C. gattii into five species based on a phylogenetic analysis of 115 isolates. While this proposal adds to the knowledge about the genetic diversity and population structure of cryptococcosis agents, the published genotypes of 2,606 strains have already revealed more genetic diversity than is encompassed by seven species. Naming every clade as a separate species at this juncture will lead to continuing nomenclatural instability. In the absence of biological differences between clades and no consensus about how DNA sequence alone can delineate a species, we recommend using " Cryptococcus neoformans species complex" and " C. gattii species complex" as a practical intermediate step, rather than creating more species. This strategy recognizes genetic diversity without creating confusion.

Improving Access to Hospice Care: Informing the Debate

PubMed Central

CARLSON, MELISSA D.A.; MORRISON, R. SEAN; BRADLEY, ELIZABETH H.

2015-01-01

The most frequently cited policy solution for improving access to hospice care for patients and families is to expand hospice eligibility criteria under the Medicare Hospice Benefit. However, the substantial implications of such a policy change have not been fully articulated or evaluated. This paper seeks to identify and describe the implications of expanding Medicare Hospice Benefit eligibility on the nature of hospice care, the cost of hospice care to the Medicare program, and the very structure of hospice and palliative care delivery in the United States. The growth in hospice has been dramatic and the central issue facing policymakers and the hospice industry is defining the appropriate target population for hospice care. As policymakers and the hospice industry discuss the future of hospice and potential changes to the Medicare Hospice Benefit, it is critical to clearly delineate the options—and the implications and challenges of each option—for improving access to hospice care for patients and families. PMID:18363486

γ -soft Ba 146 and the role of nonaxial shapes at N ≈ 90

DOE PAGES

Mitchell, A. J.; Lister, C. J.; McCutchan, E. A.; ...

2016-01-12

Low-spin states in the neutron-rich, N = 90 nuclide 146Ba were populated following β decay of 146Cs , with the goal of clarifying the development of deformation in barium isotopes through delineation of their nonyrast structures. Fission fragments of 146Cs were extracted from a 1.7-Ci 252Cf source and mass selected using the CAlifornium Rare Ion Breeder Upgrade (CARIBU) facility. Low-energy ions were deposited at the center of a box of thin β detectors, surrounded by a highly efficient high-purity Ge array. The new 146Ba decay scheme now contains 31 excited levels extending up to ~2.5 MeV excitation energy, double whatmore » was previously known. These data are compared to predictions from the interacting boson approximation (IBA) model. It appears that the abrupt shape change found at N = 90 in Sm and Gd is much more gradual in Ba and Ce, due to an enhanced role of the γ degree of freedom.« less

Feasibility of creating a high-resolution 3D diffusion tensor imaging based atlas of the human brainstem: A case study at 11.7T

PubMed Central

Aggarwal, Manisha; Zhang, Jiangyang; Pletnikova, Olga; Crain, Barbara; Troncoso, Juan; Mori, Susumu

2013-01-01

A three-dimensional stereotaxic atlas of the human brainstem based on high resolution ex vivo diffusion tensor imaging (DTI) is introduced. The atlas consists of high resolution (125–255 μm isotropic) three-dimensional DT images of the formalin-fixed brainstem acquired at 11.7T. The DTI data revealed microscopic neuroanatomical details, allowing three-dimensional visualization and reconstruction of fiber pathways including the decussation of the pyramidal tract fibers, and interdigitating fascicles of the corticospinal and transverse pontine fibers. Additionally, strong grey-white matter contrasts in the apparent diffusion coefficient (ADC) maps enabled precise delineation of grey matter nuclei in the brainstem, including the cranial nerve and the inferior olivary nuclei. Comparison with myelin-stained histology shows that at the level of resolution achieved in this study, the structural details resolved with DTI contrasts in the brainstem were comparable to anatomical delineation obtained with histological sectioning. Major neural structures delineated from DTI contrasts in the brainstem are segmented and three-dimensionally reconstructed. Further, the ex vivo DTI data are nonlinearly mapped to a widely-used in vivo human brain atlas, to construct a high-resolution atlas of the brainstem in the Montreal Neurological Institute (MNI) stereotaxic coordinate space. The results demonstrate the feasibility of developing a 3D DTI based atlas for detailed characterization of brainstem neuroanatomy with high resolution and contrasts, which will be a useful resource for research and clinical applications. PMID:23384518

Intra-temporal facial nerve centerline segmentation for navigated temporal bone surgery

NASA Astrophysics Data System (ADS)

Voormolen, Eduard H. J.; van Stralen, Marijn; Woerdeman, Peter A.; Pluim, Josien P. W.; Noordmans, Herke J.; Regli, Luca; Berkelbach van der Sprenkel, Jan W.; Viergever, Max A.

2011-03-01

Approaches through the temporal bone require surgeons to drill away bone to expose a target skull base lesion while evading vital structures contained within it, such as the sigmoid sinus, jugular bulb, and facial nerve. We hypothesize that an augmented neuronavigation system that continuously calculates the distance to these structures and warns if the surgeon drills too close, will aid in making safe surgical approaches. Contemporary image guidance systems are lacking an automated method to segment the inhomogeneous and complexly curved facial nerve. Therefore, we developed a segmentation method to delineate the intra-temporal facial nerve centerline from clinically available temporal bone CT images semi-automatically. Our method requires the user to provide the start- and end-point of the facial nerve in a patient's CT scan, after which it iteratively matches an active appearance model based on the shape and texture of forty facial nerves. Its performance was evaluated on 20 patients by comparison to our gold standard: manually segmented facial nerve centerlines. Our segmentation method delineates facial nerve centerlines with a maximum error along its whole trajectory of 0.40+/-0.20 mm (mean+/-standard deviation). These results demonstrate that our model-based segmentation method can robustly segment facial nerve centerlines. Next, we can investigate whether integration of this automated facial nerve delineation with a distance calculating neuronavigation interface results in a system that can adequately warn surgeons during temporal bone drilling, and effectively diminishes risks of iatrogenic facial nerve palsy.

Remote sensing data with the conditional latin hypercube sampling and geostatistical approach to delineate landscape changes induced by large chronological physical disturbances.

PubMed

Lin, Yu-Pin; Chu, Hone-Jay; Wang, Cheng-Long; Yu, Hsiao-Hsuan; Wang, Yung-Chieh

2009-01-01

This study applies variogram analyses of normalized difference vegetation index (NDVI) images derived from SPOT HRV images obtained before and after the ChiChi earthquake in the Chenyulan watershed, Taiwan, as well as images after four large typhoons, to delineate the spatial patterns, spatial structures and spatial variability of landscapes caused by these large disturbances. The conditional Latin hypercube sampling approach was applied to select samples from multiple NDVI images. Kriging and sequential Gaussian simulation with sufficient samples were then used to generate maps of NDVI images. The variography of NDVI image results demonstrate that spatial patterns of disturbed landscapes were successfully delineated by variogram analysis in study areas. The high-magnitude Chi-Chi earthquake created spatial landscape variations in the study area. After the earthquake, the cumulative impacts of typhoons on landscape patterns depended on the magnitudes and paths of typhoons, but were not always evident in the spatiotemporal variability of landscapes in the study area. The statistics and spatial structures of multiple NDVI images were captured by 3,000 samples from 62,500 grids in the NDVI images. Kriging and sequential Gaussian simulation with the 3,000 samples effectively reproduced spatial patterns of NDVI images. However, the proposed approach, which integrates the conditional Latin hypercube sampling approach, variogram, kriging and sequential Gaussian simulation in remotely sensed images, efficiently monitors, samples and maps the effects of large chronological disturbances on spatial characteristics of landscape changes including spatial variability and heterogeneity.

A suitable country: the relationship between Sweden's interwar population policy and family planning in postindependence India.

PubMed

Berg, Annika

2010-09-01

This article delineates a strong continuity, particularly in terms of personnel, between interwar domestic population policies and Sweden's postwar participation in international and transnational population-control programs. It argues that Swedish engagement in population control and family planning in the emerging Third World, and particularly in South Asia, was motivated by the conviction that poverty and underdevelopment must be attacked on several fronts simultaneously, with population control being one of the most important. In its first bilateral aid programs Sweden would prioritize the promotion of birth control primarily because it was still too controversial to be promoted multilaterally, not least for religious reasons; and because Swedish experts were regarded as especially liberal, rational, and secularized. Sterilization expertise played no decisive part in this continuity. When first establishing themselves in South Asia, Swedish experts would recommend the rhythm method and other contraceptive methods that depended on self-control.

Detecting and modelling structures on the micro and the macro scales: Assessing their effects on solute transport behaviour

NASA Astrophysics Data System (ADS)

Haslauer, C. P.; Bárdossy, A.; Sudicky, E. A.

2017-09-01

This paper demonstrates quantitative reasoning to separate the dataset of spatially distributed variables into different entities and subsequently characterize their geostatistical properties, properly. The main contribution of the paper is a statistical based algorithm that matches the manual distinction results. This algorithm is based on measured data and is generally applicable. In this paper, it is successfully applied at two datasets of saturated hydraulic conductivity (K) measured at the Borden (Canada) and the Lauswiesen (Germany) aquifers. The boundary layer was successfully delineated at Borden despite its only mild heterogeneity and only small statistical differences between the divided units. The methods are verified with the more heterogeneous Lauswiesen aquifer K data-set, where a boundary layer has previously been delineated. The effects of the macro- and the microstructure on solute transport behaviour are evaluated using numerical solute tracer experiments. Within the microscale structure, both Gaussian and non-Gaussian models of spatial dependence of K are evaluated. The effects of heterogeneity both on the macro- and the microscale are analysed using numerical tracer experiments based on four scenarios: including or not including the macroscale structures and optimally fitting a Gaussian or a non-Gaussian model for the spatial dependence in the micro-structure. The paper shows that both micro- and macro-scale structures are important, as in each of the four possible geostatistical scenarios solute transport behaviour differs meaningfully.

Concurrent segmentation of the prostate on MRI and CT via linked statistical shape models for radiotherapy planning

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chowdhury, Najeeb; Toth, Robert; Chappelow, Jonathan

2012-04-15

Purpose: Prostate gland segmentation is a critical step in prostate radiotherapy planning, where dose plans are typically formulated on CT. Pretreatment MRI is now beginning to be acquired at several medical centers. Delineation of the prostate on MRI is acknowledged as being significantly simpler to perform, compared to delineation on CT. In this work, the authors present a novel framework for building a linked statistical shape model (LSSM), a statistical shape model (SSM) that links the shape variation of a structure of interest (SOI) across multiple imaging modalities. This framework is particularly relevant in scenarios where accurate boundary delineations ofmore » the SOI on one of the modalities may not be readily available, or difficult to obtain, for training a SSM. In this work the authors apply the LSSM in the context of multimodal prostate segmentation for radiotherapy planning, where the prostate is concurrently segmented on MRI and CT. Methods: The framework comprises a number of logically connected steps. The first step utilizes multimodal registration of MRI and CT to map 2D boundary delineations of the prostate from MRI onto corresponding CT images, for a set of training studies. Hence, the scheme obviates the need for expert delineations of the gland on CT for explicitly constructing a SSM for prostate segmentation on CT. The delineations of the prostate gland on MRI and CT allows for 3D reconstruction of the prostate shape which facilitates the building of the LSSM. In order to perform concurrent prostate MRI and CT segmentation using the LSSM, the authors employ a region-based level set approach where the authors deform the evolving prostate boundary to simultaneously fit to MRI and CT images in which voxels are classified to be either part of the prostate or outside the prostate. The classification is facilitated by using a combination of MRI-CT probabilistic spatial atlases and a random forest classifier, driven by gradient and Haar features. Results: The authors acquire a total of 20 MRI-CT patient studies and use the leave-one-out strategy to train and evaluate four different LSSMs. First, a fusion-based LSSM (fLSSM) is built using expert ground truth delineations of the prostate on MRI alone, where the ground truth for the gland on CT is obtained via coregistration of the corresponding MRI and CT slices. The authors compare the fLSSM against another LSSM (xLSSM), where expert delineations of the gland on both MRI and CT are employed in the model building; xLSSM representing the idealized LSSM. The authors also compare the fLSSM against an exclusive CT-based SSM (ctSSM), built from expert delineations of the gland on CT alone. In addition, two LSSMs trained using trainee delineations (tLSSM) on CT are compared with the fLSSM. The results indicate that the xLSSM, tLSSMs, and the fLSSM perform equivalently, all of them out-performing the ctSSM. Conclusions: The fLSSM provides an accurate alternative to SSMs that require careful expert delineations of the SOI that may be difficult or laborious to obtain. Additionally, the fLSSM has the added benefit of providing concurrent segmentations of the SOI on multiple imaging modalities.« less

Project management - Factors leading to success or failure

NASA Technical Reports Server (NTRS)

Kelley, A. J.; Murphy, D. C.

1974-01-01

This paper presents initial findings of a study designed to detail the relationships among situational, structural, and process variables as they relate to project effectiveness. In the paper, emphasis is placed on delineating those variables which tend to improve and those which tend to impede project effectiveness.

Joint inversion of seismic and gravity data for imaging seismic velocity structure of the crust and upper mantle beneath Utah, United States

NASA Astrophysics Data System (ADS)

Syracuse, E. M.; Zhang, H.; Maceira, M.

2017-10-01

We present a method for using any combination of body wave arrival time measurements, surface wave dispersion observations, and gravity data to simultaneously invert for three-dimensional P- and S-wave velocity models. The simultaneous use of disparate data types takes advantage of the differing sensitivities of each data type, resulting in a comprehensive and higher resolution three-dimensional geophysical model. In a case study for Utah, we combine body wave first arrivals mainly from the USArray Transportable Array, Rayleigh wave group and phase velocity dispersion data, and Bouguer gravity anomalies to invert for crustal and upper mantle structure of the region. Results show clear delineations, visible in both P- and S-wave velocities, between the three main tectonic provinces in the region. Without the inclusion of the surface wave and gravity constraints, these delineations are less clear, particularly for S-wave velocities. Indeed, checkerboard tests confirm that the inclusion of the additional datasets dramatically improves S-wave velocity recovery, with more subtle improvements to P-wave velocity recovery, demonstrating the strength of the method in successfully recovering seismic velocity structure from multiple types of constraints.

Delineating Substrate Diversity of Disparate Short-Chain Dehydrogenase Reductase from Debaryomyces hansenii.

PubMed

Ghatak, Arindam; Bharatham, Nagakumar; Shanbhag, Anirudh P; Datta, Santanu; Venkatraman, Janani

2017-01-01

Short-chain dehydrogenase reductases (SDRs) have been utilized for catalyzing the reduction of many aromatic/aliphatic prochiral ketones to their respective alcohols. However, there is a paucity of data that elucidates their innate biological role and diverse substrate space. In this study, we executed an in-depth biochemical characterization and substrate space mapping (with 278 prochiral ketones) of an unannotated SDR (DHK) from Debaryomyces hansenii and compared it with structurally and functionally characterized SDR Synechococcus elongatus. PCC 7942 FabG to delineate its industrial significance. It was observed that DHK was significantly more efficient than FabG, reducing a diverse set of ketones albeit at higher conversion rates. Comparison of the FabG structure with a homology model of DHK and a docking of substrate to both structures revealed the presence of additional flexible loops near the substrate binding site of DHK. The comparative elasticity of the cofactor and substrate binding site of FabG and DHK was experimentally substantiated using differential scanning fluorimetry. It is postulated that the loop flexibility may account for the superior catalytic efficiency of DHK although the positioning of the catalytic triad is conserved.

Delineating Substrate Diversity of Disparate Short-Chain Dehydrogenase Reductase from Debaryomyces hansenii

PubMed Central

Ghatak, Arindam; Bharatham, Nagakumar; Shanbhag, Anirudh P.; Datta, Santanu; Venkatraman, Janani

2017-01-01

Short-chain dehydrogenase reductases (SDRs) have been utilized for catalyzing the reduction of many aromatic/aliphatic prochiral ketones to their respective alcohols. However, there is a paucity of data that elucidates their innate biological role and diverse substrate space. In this study, we executed an in-depth biochemical characterization and substrate space mapping (with 278 prochiral ketones) of an unannotated SDR (DHK) from Debaryomyces hansenii and compared it with structurally and functionally characterized SDR Synechococcus elongatus. PCC 7942 FabG to delineate its industrial significance. It was observed that DHK was significantly more efficient than FabG, reducing a diverse set of ketones albeit at higher conversion rates. Comparison of the FabG structure with a homology model of DHK and a docking of substrate to both structures revealed the presence of additional flexible loops near the substrate binding site of DHK. The comparative elasticity of the cofactor and substrate binding site of FabG and DHK was experimentally substantiated using differential scanning fluorimetry. It is postulated that the loop flexibility may account for the superior catalytic efficiency of DHK although the positioning of the catalytic triad is conserved. PMID:28107498

Towards sustainable management of huntable migratory waterbirds in Europe

USGS Publications Warehouse

Madsen, Jesper; Guillemain, Matthieu; Nagy, Szabolcs; Defos du Rau, Pierre; Mondain-Monval, Jean-Yves; Griffin, Cy; Williams, James Henty; Bunnefeld, Nils; Czajkowski, Alexandre; Hearn, Richard; Grauer, Andreas; Alhainen, Mikko; Middleton, Angus; Johnson, Fred A.

2015-01-01

The EU Birds Directive and the African-Eurasian Waterbird Agreement provide an adequate legal framework for sustainable management of migratory waterbird populations. The main shortcoming of both instruments is that it leaves harvest decisions of a shared resource to individual Member States and Contracting Parties without providing a shared information base and mechanism to assess the impact of harvest and coordinate actions in relation to mutually agreed objectives. A recent update of the conservation status of waterbirds in the EU shows that almost half of the populations of species listed on Annex II of the Birds Directive have a declining short-term trend and over half of them are listed in Columns A and B of AEWA. This implies that their hunting could either only continue under the framework of an adaptive harvest management plan or their hunting should be regulated with the view of restoring them in favourable conservation status. We argue that a structured approach to decision-making (such as adaptive management) is needed, supported with adequate organisational structures at flyway scale. We review the experience with such an approach in North America and assess the applicability of a similar approach in the European context. We show there is no technical reason why adaptive harvest management could be not applied in the EU or even AEWA context. We demonstrate that an informed approach to setting allowable harvests does not require detailed demographic information. Essential to the process, however, are estimates of either the observed growth rate from a monitoring program or the growth rate expected under ideal conditions. In addition, periodic estimates of population size are needed, as well as either empirical information or reasonable assumptions about the form of density dependence. We show that such information exists for many populations, but improvements are needed to improve geographic coverage, reliability and timely data availability. We highlight the importance of the International Waterbird Census and specialised goose and seaduck monitoring in estimating population sizes and observed growth rate of the populations. We encourage further investments into the development of these schemes. We also recognise the importance of migration studies to improve our understanding of delineations of populations. We also highlight that, with a few exceptions, the available data does not allow the European Commission, competent authorities of the Members States or other AEWA Contracting Parties to assess levels of harvest and their sustainability and, therefore, regulate hunting accordingly. Therefore, we recommend that annual reporting on 2 harvest levels of waterbird populations would be gradually introduced in the EU and the AEWA region. We propose that future AEWA and EU action plans and management plans for Annex II species should apply the principles of adaptive harvest management framework and make provisions for setting up adequate monitoring and information management systems and organisational structures to manage the decision-making process. We suggest that internationally coordinated management structures are established to facilitate dialogue, learning and communication between stakeholders with different interests and cultural backgrounds.

Evaluating segmentation error without ground truth.

PubMed

Kohlberger, Timo; Singh, Vivek; Alvino, Chris; Bahlmann, Claus; Grady, Leo

2012-01-01

The automatic delineation of the boundaries of organs and other anatomical structures is a key component of many medical image processing systems. In this paper we present a generic learning approach based on a novel space of segmentation features, which can be trained to predict the overlap error and Dice coefficient of an arbitrary organ segmentation without knowing the ground truth delineation. We show the regressor to be much stronger a predictor of these error metrics than the responses of probabilistic boosting classifiers trained on the segmentation boundary. The presented approach not only allows us to build reliable confidence measures and fidelity checks, but also to rank several segmentation hypotheses against each other during online usage of the segmentation algorithm in clinical practice.

Spatial and temporal variability in growth of southern flounder (Paralichthys lethostigma)

USGS Publications Warehouse

Midway, Stephen R.; Wagner, Tyler; Arnott, Stephen A.; Biondo, Patrick; Martinez-Andrade, Fernando; Wadsworth, Thomas F.

2015-01-01

Delineation of stock structure is important for understanding the ecology and management of many fish populations, particularly those with wide-ranging distributions and high levels of harvest. Southern flounder (Paralichthys lethostigma) is a popular commercial and recreational species along the southeast Atlantic coast and Gulf of Mexico, USA. Recent studies have provided genetic and otolith morphology evidence that the Gulf of Mexico and Atlantic Ocean stocks differ. Using age and growth data from four states (Texas, Alabama, South Carolina, and North Carolina) we expanded upon the traditional von Bertalanffy model in order to compare growth rates of putative geographic stocks of southern flounder. We improved the model fitting process by adding a hierarchical Bayesian framework to allow each parameter to vary spatially or temporally as a random effect, as well as log transforming the three model parameters (L∞, K, andt0). Multiple comparisons of parameters showed that growth rates varied (even within states) for females, but less for males. Growth rates were also consistent through time, when long-term data were available. Since within-basin populations are thought to be genetically well-mixed, our results suggest that consistent small-scale environmental conditions (i.e., within estuaries) likely drive growth rates and should be considered when developing broader scale management plans.

Region 9 2010 Census Web Service

EPA Pesticide Factsheets

This web service displays data collected during the 2010 U.S. Census. The data are organized into layers representing Tract, Block, and Block Group visualizations. Geography The TIGER Line Files are feature classes and related database files that are an extract of selected geographic and cartographic information from the U.S. Census Bureau's Master Address File / Topologically Integrated Geographic Encoding and Referencing (MAF/TIGER) Database (MTDB). The MTDB represents a seamless national file with no overlaps or gaps between parts, however, each TIGER Line File is designed to stand alone as an independent data set, or they can be combined to cover the entire nation. Census tracts are small, relatively permanent statistical subdivisions of a county or equivalent entity, and were defined by local participants as part of the 2010 Census Participant Statistical Areas Program. The Census Bureau delineated the census tracts in situations where no local participant existed or where all the potential participants declined to participate. The primary purpose of census tracts is to provide a stable set of geographic units for the presentation of census data and comparison back to previous decennial censuses. Census tracts generally have a population size between 1,200 and 8,000 people, with an optimum size of 4,000 people. When first delineated, census tracts were designed to be homogeneous with respect to population characteristics, economic status

SCHEMA computational design of virus capsid chimeras: calibrating how genome packaging, protection, and transduction correlate with calculated structural disruption.

PubMed

Ho, Michelle L; Adler, Benjamin A; Torre, Michael L; Silberg, Jonathan J; Suh, Junghae

2013-12-20

Adeno-associated virus (AAV) recombination can result in chimeric capsid protein subunits whose ability to assemble into an oligomeric capsid, package a genome, and transduce cells depends on the inheritance of sequence from different AAV parents. To develop quantitative design principles for guiding site-directed recombination of AAV capsids, we have examined how capsid structural perturbations predicted by the SCHEMA algorithm correlate with experimental measurements of disruption in seventeen chimeric capsid proteins. In our small chimera population, created by recombining AAV serotypes 2 and 4, we found that protection of viral genomes and cellular transduction were inversely related to calculated disruption of the capsid structure. Interestingly, however, we did not observe a correlation between genome packaging and calculated structural disruption; a majority of the chimeric capsid proteins formed at least partially assembled capsids and more than half packaged genomes, including those with the highest SCHEMA disruption. These results suggest that the sequence space accessed by recombination of divergent AAV serotypes is rich in capsid chimeras that assemble into 60-mer capsids and package viral genomes. Overall, the SCHEMA algorithm may be useful for delineating quantitative design principles to guide the creation of libraries enriched in genome-protecting virus nanoparticles that can effectively transduce cells. Such improvements to the virus design process may help advance not only gene therapy applications but also other bionanotechnologies dependent upon the development of viruses with new sequences and functions.

SCHEMA computational design of virus capsid chimeras: calibrating how genome packaging, protection, and transduction correlate with calculated structural disruption

PubMed Central

Ho, Michelle L.; Adler, Benjamin A.; Torre, Michael L.; Silberg, Jonathan J.; Suh, Junghae

2013-01-01

Adeno-associated virus (AAV) recombination can result in chimeric capsid protein subunits whose ability to assemble into an oligomeric capsid, package a genome, and transduce cells depends on the inheritance of sequence from different AAV parents. To develop quantitative design principles for guiding site-directed recombination of AAV capsids, we have examined how capsid structural perturbations predicted by the SCHEMA algorithm correlate with experimental measurements of disruption in seventeen chimeric capsid proteins. In our small chimera population, created by recombining AAV serotypes 2 and 4, we found that protection of viral genomes and cellular transduction were inversely related to calculated disruption of the capsid structure. Interestingly, however, we did not observe a correlation between genome packaging and calculated structural disruption; a majority of the chimeric capsid proteins formed at least partially assembled capsids and more than half packaged genomes, including those with the highest SCHEMA disruption. These results suggest that the sequence space accessed by recombination of divergent AAV serotypes is rich in capsid chimeras that assemble into 60-mer capsids and package viral genomes. Overall, the SCHEMA algorithm may be useful for delineating quantitative design principles to guide the creation of libraries enriched in genome-protecting virus nanoparticles that can effectively transduce cells. Such improvements to the virus design process may help advance not only gene therapy applications, but also other bionanotechnologies dependent upon the development of viruses with new sequences and functions. PMID:23899192

Remote sensing of geobotanical relations in Georgia

NASA Technical Reports Server (NTRS)

Arden, D. D., Jr.; Westra, R. N.

1977-01-01

The application of remote sensing to geological investigations, with special attention to geobotanical factors, was evaluated. The general areas of investigation included: (1) recognition of mineral deposits; (2) geological mapping; (3) delineation of geological structure, including areas of complex tectonics; and (4) limestone areas where ground withdrawal had intensified surface collapse.

Environment, energy, and society

DOE Office of Scientific and Technical Information (OSTI.GOV)

Humphrey, C.R.; Buttel, F.R.

1986-01-01

This book delineates the major ways in which human society and the environment affect each other. To study the structure of societies, it employs three conceptual models, or sociological paradigms, conservative, liberal, and radical. The book explains the courses in environmental sociology, international development, natural resources, agriculture, and urban or regional planning.

Noneconomic Analysis Considerations for Management and Information System for Occupational Education.

ERIC Educational Resources Information Center

Creager, John A.

As the first of two papers delineating the design of Massachusetts' Management and Information System for Occupational Education (MISOE), these specific dimensions of MISOE structure and function are considered: (1) the distinction between economic and noneconomic analysis, (2) distinctions among census, sample, and other data, (3) the distinction…

The Influence of Syllable Onset Complexity and Syllable Frequency on Speech Motor Control

ERIC Educational Resources Information Center

Riecker, Axel; Brendel, Bettina; Ziegler, Wolfram; Erb, Michael; Ackermann, Hermann

2008-01-01

Functional imaging studies have delineated a "minimal network for overt speech production," encompassing mesiofrontal structures (supplementary motor area, anterior cingulate gyrus), bilateral pre- and postcentral convolutions, extending rostrally into posterior parts of the inferior frontal gyrus (IFG) of the language-dominant hemisphere, left…

Gene flow between divergent cereal - and grass-specific lineages of the rice blast fungus Magnaporthe oryzae

USDA-ARS?s Scientific Manuscript database

Delineating species and epidemic lineages in fungal plant pathogens is critical to our understanding of disease emergence and the structure of fungal biodiversity, and also informs international regulatory decisions. Pyricularia oryzae (syn. Magnaporthe oryzae) is a multi-host pathogen that infects ...

Facial Structure Analysis Separates Autism Spectrum Disorders into Meaningful Clinical Subgroups

ERIC Educational Resources Information Center

Obafemi-Ajayi, Tayo; Miles, Judith H.; Takahashi, T. Nicole; Qi, Wenchuan; Aldridge, Kristina; Zhang, Minqi; Xin, Shi-Qing; He, Ying; Duan, Ye

2015-01-01

Varied cluster analysis were applied to facial surface measurements from 62 prepubertal boys with essential autism to determine whether facial morphology constitutes viable biomarker for delineation of discrete Autism Spectrum Disorders (ASD) subgroups. Earlier study indicated utility of facial morphology for autism subgrouping (Aldridge et al. in…

An Exploration of Teachers' Agency and Social Relationships within Dyslexia-Support Provision in an English Secondary School

ERIC Educational Resources Information Center

Ross, Helen

2017-01-01

This article explores teachers' experiences of dyslexia and classroom interventions via lesson observations and semi-structured interviews. These experiences were analysed through a Bourdieusien lens, based on Jenkins's "levels of interaction", to delineate power relationships inherent in classroom interactions, teachers' interactions…

Vertical Cable Seismic (VCS) Survey for SMS exploration in Izena Cauldron, Okinawa-Trough

NASA Astrophysics Data System (ADS)

Asakawa, Eiichi; Murakami, Fumitoshi; Tsukahara, Hitoshi; Mizohata, Shigeharu; Tara, Kenji

2015-04-01

In 2014, the Japanese government started the Cross-ministerial Strategic Innovation Promotion Program (SIP), which includes 'New-generation Offshore Exploration Techniques' as an area of interest. We proposed the Vertical Cable Seismic (VCS) survey technique for this program, especially for the exploration of Seafloor Massive Sulfides (SMS). VCS is a reflection seismic method that uses hydrophone arrays vertically moored from the seafloor to record acoustic waves generated by various acoustic sources. This method is useful to delineate detailed structures in a spatially-limited area below the seabed in the deep sea where conventional surface seismic is not effective. We have been developing an autonomous VCS system with the financial support of the Japanese government since 2009. We have carried out several VCS surveys and completed our VCS system. Izena Cauldron, Okinawa Trough is one of the most promising SMS areas around Japan. There are two high potential areas, the north and south mound. We carried out the first VCS survey around the north mound in 2011 and the second survey around the south mound in 2013 respectively. The first VCS survey in Izena Cauldron was carried out using a GI gun in September, 2011, with the objective of surveying the large-scale and deeper structure of the hydrothermal system. The water depth was 1,500-1,600m. Four VCS systems were deployed. The shooting lines covered an area of 9 km x 9 km with a shooting interval of about 25m and line spacing of 200m to 400m. In the second survey, we used a high-voltage sparker. The objective is to explore very shallow parts to delineate very thin SMS deposits. The survey area was about 4 km x 4km with a 12.5 m shooting interval and 100m to 200m line spacing. Three VCS systems were deployed in this survey. The result of the first GI gun VCS survey was a 3D PSDM volume of the subsurface structure. It extends 2,000m horizontally and down to 1,500m in depth. Further, by re-processing the data with a higher resolution to focus the very shallow parts, we found that this survey has enough potential to delineate the SMS deposits itself. In the second high-voltage sparker VCS survey we obtained high resolution field records and a very high resolution 3D volume down to 150m over an area of 400m x 200m. These results successfully delineated sub-seabed structures that suggest the existence of reflections about 30m beneath the seabed. This suggests the top interface of a buried SMS deposit. The detailed results of the both surveys are consistent with the information from a well drilled in the Izena Cauldron by JOGMEC These VCS survey results provide us useful SMS exploration information, and demonstrate that VCS is a suitable reflection seismic method to obtain high resolution 3D volumes just below the seabed. We consider our VCS system to have high potential to delineate SMS structures. The system will continue to be developed as part of the SIP project, along with other geophysical exploration techniques such as EM, magnetic and gravity.

A systematic review of the neurobiological underpinnings of borderline personality disorder (BPD) in childhood and adolescence.

PubMed

Winsper, Catherine; Marwaha, Steven; Lereya, Suzet Tanya; Thompson, Andrew; Eyden, Julie; Singh, Swaran P

2016-12-01

Contemporary theories for the aetiology of borderline personality disorder (BPD) take a lifespan approach asserting that inborn biological predisposition is potentiated across development by environmental risk factors. In this review, we present and critically evaluate evidence on the neurobiology of BPD in childhood and adolescence, compare this evidence to the adult literature, and contextualise within a neurodevelopmental framework. A systematic review was conducted to identify studies examining the neurobiological (i.e. genetic, structural neuroimaging, neurophysiological, and neuropsychological) correlates of BPD symptoms in children and adolescents aged 19 years or under. We identified, quality assessed, and narratively summarised 34 studies published between 1980 and June 2016. Similar to findings in adult populations, twin studies indicated moderate to high levels of heritability of BPD, and there was some evidence for gene-environment interactions. Also consistent with adult reports is that some adolescents with BPD demonstrated structural (grey and white matter) alterations in frontolimbic regions and neuropsychological abnormalities (i.e. reduced executive function and disturbances in social cognition). These findings suggest that neurobiological abnormalities observed in adult BPD may not solely be the consequence of chronic morbidity or prolonged medication use. They also provide tentative support for neurodevelopmental theories of BPD by demonstrating that neurobiological markers may be observed from childhood onwards and interact with environmental factors to increase risk of BPD in young populations. Prospective studies with a range of repeated measures are now required to elucidate the temporal unfurling of neurobiological features and further delineate the complex pathways to BPD.

Learning-based prediction of gestational age from ultrasound images of the fetal brain.

PubMed

Namburete, Ana I L; Stebbing, Richard V; Kemp, Bryn; Yaqub, Mohammad; Papageorghiou, Aris T; Alison Noble, J

2015-04-01

We propose an automated framework for predicting gestational age (GA) and neurodevelopmental maturation of a fetus based on 3D ultrasound (US) brain image appearance. Our method capitalizes on age-related sonographic image patterns in conjunction with clinical measurements to develop, for the first time, a predictive age model which improves on the GA-prediction potential of US images. The framework benefits from a manifold surface representation of the fetal head which delineates the inner skull boundary and serves as a common coordinate system based on cranial position. This allows for fast and efficient sampling of anatomically-corresponding brain regions to achieve like-for-like structural comparison of different developmental stages. We develop bespoke features which capture neurosonographic patterns in 3D images, and using a regression forest classifier, we characterize structural brain development both spatially and temporally to capture the natural variation existing in a healthy population (N=447) over an age range of active brain maturation (18-34weeks). On a routine clinical dataset (N=187) our age prediction results strongly correlate with true GA (r=0.98,accurate within±6.10days), confirming the link between maturational progression and neurosonographic activity observable across gestation. Our model also outperforms current clinical methods by ±4.57 days in the third trimester-a period complicated by biological variations in the fetal population. Through feature selection, the model successfully identified the most age-discriminating anatomies over this age range as being the Sylvian fissure, cingulate, and callosal sulci. Copyright © 2015 The Authors. Published by Elsevier B.V. All rights reserved.

Hydrostratigraphic Framework and Selection and Correlation of Geophysical Log Markers in the Surficial Aquifer System, Palm Beach County, Florida

USGS Publications Warehouse

Reese, Ronald S.; Wacker, Michael A.

2007-01-01

The surficial aquifer system is the major source of freshwater for public water supply in Palm Beach County, Florida, yet many previous studies of the hydrogeology of this aquifer system have focused only on the eastern one-half to one-third of the county in the more densely populated coastal area (Land and others, 1973; Swayze and others, 1980; Swayze and Miller, 1984; Shine and others, 1989). Population growth in the county has resulted in the westward expansion of urbanized areas into agricultural areas and has created new demands on the water resources of the county. Additionally, interest in surface-water resources of central and western areas of the county has increased. In these areas, plans for additional surface-water storage reservoirs are being made under the Comprehensive Everglades Restoration Plan originally proposed by the U.S. Army Corps of Engineers and the South Florida Water Management District (1999), and stormwater treatment areas have been constructed by the South Florida Water Management District. Surface-water and ground-water interactions in the Everglades are thought to be important to water budgets, water quality, and ecology (Harvey and others, 2002). Most of the previous hydrogeologic and ground-water flow simulation studies of the surficial aquifer system have not utilized a hydrostratigraphic framework, in which stratigraphic or sequence stratigraphic units, such as those proposed in Cunningham and others (2001), are delineated in this stratigraphically complex aquifer system. A thick zone of secondary permeability mapped by Swayze and Miller (1984) was not subdivided and was identified as only being within the Anastasia Formation of Pleistocene age. Miller (1987) published 11 geologic sections of the surficial aquifer system, but did not delineate any named stratigraphic units in these sections. This limited interpretation has resulted, in part, from the complex facies changes within rocks and sediments of the surficial aquifer system and the seemingly indistinct and repetitious nature of the most common lithologies, which include sand, shell, sandstone, and limestone. Model construction and layer definition in a simulation of ground-water flow within the surficial aquifer system of Palm Beach County utilized only the boundaries of one or two major hydrogeologic zones, such as the Biscayne aquifer and surficial aquifer system; otherwise layers were defined by average elevations rather than geologic structure or stratigraphy (Shine and others, 1989). Additionally, each major permeable zone layer in the model was assumed to have constant hydraulic conductivity with no allowance for the possibility of discrete (thin) flow zones within the zone. The key to understanding the spatial distribution and hydraulic connectivity of permeable zones in the surficial aquifer system beneath Palm Beach County is the development of a stratigraphic framework based on a consistent method of county-wide correlation. Variability in hydraulic properties in the system needs to be linked to the stratigraphic units delineated in this framework, and proper delineation of the hydrostratigraphic framework should provide a better understanding and simulation of the ground-water flow system. In 2004, the U.S. Geological Survey, in cooperation with the South Florida Water Management District, initiated an investigation to develop a hydrostratigraphic framework for the surficial aquifer system in Palm Beach County.

Unique sail-like structure of cor triatriatum dexter in three-dimensional echocardiogram.

PubMed

Low, Ting Ting; Uy, Celia Catherine C; Wong, Raymond Ching Chiew

2014-08-01

Cor triatriatum dexter (CTD) is an extremely rare congenital condition arising from the persistence of the right valve of the sinus venosus. It divides the right atrium (RA) into 2 separate chambers. We report a case of a 50-year-old man who had an incidental finding of CTD on transesophageal echocardiogram. An incomplete membrane of the RA was seen, and three-dimensional echocardiogram delineated the structure clearly as a triangular sail-like structure with multiple orifices and a fenestration. © 2013, Wiley Periodicals, Inc.

Advanced composites technology program

NASA Technical Reports Server (NTRS)

Davis, John G., Jr.

1993-01-01

This paper provides a brief overview of the NASA Advanced Composites Technology (ACT) Program. Critical technology issues that must be addressed and solved to develop composite primary structures for transport aircraft are delineated. The program schedule and milestones are included. Work completed in the first 3 years of the program indicates the potential for achieving composite structures that weigh less and are cost effective relative to conventional aluminum structure. Selected technical accomplishments are noted. Readers who are seeking more in-depth technical information should study the other papers included in these proceedings.

The RDoC initiative and the structure of psychopathology.

PubMed

Krueger, Robert F; DeYoung, Colin G

2016-03-01

The NIMH Research Domain Criteria (RDoC) project represents a welcome effort to circumvent the limitations of psychiatric categories as phenotypes for psychopathology research. Here, we describe the hierarchical and dimensional structure of phenotypic psychopathology and illustrate how this structure provides phenotypes suitable for RDoC research on neural correlates of psychopathology. A hierarchical and dimensional approach to psychopathology phenotypes holds great promise for delineating connections between neuroscience constructs and the patterns of affect, cognition, and behavior that constitute manifest psychopathology. © 2016 Society for Psychophysiological Research.

Comorbidity of Migraine

PubMed Central

BIÇAKCI, Şebnem

2013-01-01

Migraine is a common neurological disorder and can be severely disabling during attacks. The highest prevalence occurs between the ages of 25 and 55 years. Prior studies have found that migraine occurs together with other illnesses at a greater coincidental rate than is seen in the general population. These occurrences are called “comorbidities”. To delineate the comorbidities of migraine is important, because it can help improve treatment strategies and the understanding of the possible pathophysiology of migraine. PMID:28360578

HTLV-I Seroconversion Study.

DTIC Science & Technology

1992-08-25

was delineated, with population-based seroprevalence rates less than 1% in Northern Japan and as high as 37% in the ATL-endemic area of Nagasaki (2...and Africa (Figure 1 in Appendices). These surveys have documented the focal patternI of seropositivity and disease occurrence now known to be...highest endemic rates of HTLV-I in the world, with approximately 15%-40% of adults being infected (2,9,13). It is unique in the military as it is the

Delimiting Species-Poor Data Sets using Single Molecular Markers: A Study of Barcode Gaps, Haplowebs and GMYC.

PubMed

Dellicour, Simon; Flot, Jean-François

2015-11-01

Most single-locus molecular approaches to species delimitation available to date have been designed and tested on data sets comprising at least tens of species, whereas the opposite case (species-poor data sets for which the hypothesis that all individuals are conspecific cannot by rejected beforehand) has rarely been the focus of such attempts. Here we compare the performance of barcode gap detection, haplowebs and generalized mixed Yule-coalescent (GMYC) models to delineate chimpanzees and bonobos using nuclear sequence markers, then apply these single-locus species delimitation methods to data sets of one, three, or six species simulated under a wide range of population sizes, speciation rates, mutation rates and sampling efforts. Our results show that barcode gap detection and GMYC models are unable to delineate species properly in data sets composed of one or two species, two situations in which haplowebs outperform them. For data sets composed of three or six species, bGMYC and haplowebs outperform the single-threshold and multiple-threshold versions of GMYC, whereas a clear barcode gap is only observed when population sizes and speciation rates are both small. The latter conditions represent a "sweet spot" for molecular taxonomy where all the single-locus approaches tested work well; however, the performance of these methods decreases strongly when population sizes and speciation rates are high, suggesting that multilocus approaches may be necessary to tackle such cases. © The Author(s) 2015. Published by Oxford University Press, on behalf of the Society of Systematic Biologists. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Health advocacy.

PubMed

Hubinette, Maria; Dobson, Sarah; Scott, Ian; Sherbino, Jonathan

2017-02-01

In the medical profession, activities related to ensuring access to care, navigating the system, mobilizing resources, addressing health inequities, influencing health policy and creating system change are known as health advocacy. Foundational concepts in health advocacy include social determinants of health and health inequities. The social determinants of health (i.e. the conditions in which people live and work) account for a significant proportion of an individual's and a population's health outcomes. Health inequities are disparities in health between populations, perpetuated by economic, social, and political forces. Although it is clear that efforts to improve the health of an individual or population must consider "upstream" factors, how this is operationalized in medicine and medical education is controversial. There is a lack of clarity around how health advocacy is delineated, how physicians' scope of responsibility is defined and how teaching and assessment is conceptualized and enacted. Numerous curricular interventions have been described in the literature; however, regardless of the success of isolated interventions, understanding health advocacy instruction, assessment and evaluation will require a broader examination of processes, practices and values throughout medicine and medical education. To support the instruction, assessment and evaluation of health advocacy, a novel framework for health advocacy is introduced. This framework was developed for several purposes: defining and delineating different types and approaches to advocacy, generating a "roadmap" of possible advocacy activities, establishing shared language and meaning to support communication and collaboration across disciplines and providing a tool for the assessment of learners and for the evaluation of teaching and programs. Current approaches to teaching and assessment of health advocacy are outlined, as well as suggestions for future directions and considerations.

Metropolitan area of Chicago

NASA Image and Video Library

1973-09-18

SL3-88-222 (18 Sept. 1973) --- The metropolitan area of Chicago is encompassed in this Skylab 3 Earth Resources Experiments Package (EREP) S190-B photograph taken on Sept. 18, 1973 from the Skylab space station cluster in Earth orbit. The surrounding major cities of Aurora and Joliet, Illinois; Hammond, Gary and East Chicago, Indiana, are easily delineated. The photograph reveals the following: (1) Cultural differentiation of commercial, industrial and residential areas for use in population and social studies in micro-macro community planning and in cultural pattern studies in the improvement of urban areas. (Aurora is one of 27 census cities of interest to Robert Alexander, a principal investigator. Alexander is with the U.S. Geological Survey). (2) The transportation network with major corridors and their interchanges, primary and feeder streets for use in network analysis and in the development of models for population movement and land use projection. (3) The agricultural lands for land use identification on crop inventory analysis; airports for use in delineation of service and infringement of major man-made features that affect ecosystem balance (support to environmental impact studies). (4) Air and water plumes for use in case studies, natural and man-made differentiation of pollution sources, in support of model development and in ecosystem research studies on the effects of pollution. (5) Recreational centers for use in relating recreational centers to population centers, establishing possible demands and in development of possible future recreational centers to support the demand. All EREP photography is available to the public through the Department of Interior?s Earth Resources Observations Systems Data Center, Sioux Falls, South Dakota, 57198. Photo credit: NASA

Ultrasonic evaluation of the neonatal brain.

PubMed

Johnson, M L; Rumack, C M

1980-04-01

Ultrasound examination of the infant brain has been performed in selected medical centers for many years. However, the equipment necessary for obtaining satisfactory visualization of the brain has only recently become commercially available. Currently, ultrasonography is an excellent, noninvasive, inexpensive, rapid, and safe imaging modality for the evaluation of hydrocephalus and other pathologic conditions of the neonatal brain. Ventricular size can often be evaluated in infants up to two or three years of age, but a detailed image of the brain parenchyma becomes more difficult to obtain in a term infant after the first two to three months of life. With the use of the water path and high resolution, real-time systems and with the delineation of structures by multiple projections, (axial, coronal, sagittal and occipital), complex abnormalities may be delineated.

Evaluation of an automatic segmentation algorithm for definition of head and neck organs at risk.

PubMed

Thomson, David; Boylan, Chris; Liptrot, Tom; Aitkenhead, Adam; Lee, Lip; Yap, Beng; Sykes, Andrew; Rowbottom, Carl; Slevin, Nicholas

2014-08-03

The accurate definition of organs at risk (OARs) is required to fully exploit the benefits of intensity-modulated radiotherapy (IMRT) for head and neck cancer. However, manual delineation is time-consuming and there is considerable inter-observer variability. This is pertinent as function-sparing and adaptive IMRT have increased the number and frequency of delineation of OARs. We evaluated accuracy and potential time-saving of Smart Probabilistic Image Contouring Engine (SPICE) automatic segmentation to define OARs for salivary-, swallowing- and cochlea-sparing IMRT. Five clinicians recorded the time to delineate five organs at risk (parotid glands, submandibular glands, larynx, pharyngeal constrictor muscles and cochleae) for each of 10 CT scans. SPICE was then used to define these structures. The acceptability of SPICE contours was initially determined by visual inspection and the total time to modify them recorded per scan. The Simultaneous Truth and Performance Level Estimation (STAPLE) algorithm created a reference standard from all clinician contours. Clinician, SPICE and modified contours were compared against STAPLE by the Dice similarity coefficient (DSC) and mean/maximum distance to agreement (DTA). For all investigated structures, SPICE contours were less accurate than manual contours. However, for parotid/submandibular glands they were acceptable (median DSC: 0.79/0.80; mean, maximum DTA: 1.5 mm, 14.8 mm/0.6 mm, 5.7 mm). Modified SPICE contours were also less accurate than manual contours. The utilisation of SPICE did not result in time-saving/improve efficiency. Improvements in accuracy of automatic segmentation for head and neck OARs would be worthwhile and are required before its routine clinical implementation.

Delineation of a quick clay zone at Smørgrav, Norway, with electromagnetic methods under geotechnical constraints

NASA Astrophysics Data System (ADS)

Kalscheuer, Thomas; Bastani, Mehrdad; Donohue, Shane; Persson, Lena; Aspmo Pfaffhuber, Andreas; Reiser, Fabienne; Ren, Zhengyong

2013-05-01

In many coastal areas of North America and Scandinavia, post-glacial clay sediments have emerged above sea level due to iso-static uplift. These clays are often destabilised by fresh water leaching and transformed to so-called quick clays as at the investigated area at Smørgrav, Norway. Slight mechanical disturbances of these materials may trigger landslides. Since the leaching increases the electrical resistivity of quick clay as compared to normal marine clay, the application of electromagnetic (EM) methods is of particular interest in the study of quick clay structures. For the first time, single and joint inversions of direct-current resistivity (DCR), radiomagnetotelluric (RMT) and controlled-source audiomagnetotelluric (CSAMT) data were applied to delineate a zone of quick clay. The resulting 2-D models of electrical resistivity correlate excellently with previously published data from a ground conductivity metre and resistivity logs from two resistivity cone penetration tests (RCPT) into marine clay and quick clay. The RCPT log into the central part of the quick clay identifies the electrical resistivity of the quick clay structure to lie between 10 and 80 Ω m. In combination with the 2-D inversion models, it becomes possible to delineate the vertical and horizontal extent of the quick clay zone. As compared to the inversions of single data sets, the joint inversion model exhibits sharper resistivity contrasts and its resistivity values are more characteristic of the expected geology. In our preferred joint inversion model, there is a clear demarcation between dry soil, marine clay, quick clay and bedrock, which consists of alum shale and limestone.

Using satellite radiotelemetry data to delineate and manage wildlife populations

USGS Publications Warehouse

Amstrup, Steven C.; McDonald, T.L.; Durner, George M.

2004-01-01

The greatest promise of radiotelemetry always has been a better understanding of animal movements. Telemetry has helped us know when animals are active, how active they are, how far and how fast they move, the geographic areas they occupy, and whether individuals vary in these traits. Unfortunately, the inability to estimate the error in animals utilization distributions (UDs), has prevented probabilistic linkage of movements data, which are always retrospective, with future management actions. We used the example of the harvested population of polar bears (Ursus maritimus) in the Southern Beaufort Sea to illustrate a method that provides that linkage. We employed a 2-dimensional Gaussian kernel density estimator to smooth and scale frequencies of polar bear radio locations within cells of a grid overlying our study area. True 2-dimensional smoothing allowed us to create accurate descriptions of the UDs of individuals and groups of bears. We used a new method of clustering, based upon the relative use collared bears made of each cell in our grid, to assign individual animals to populations. We applied the fast Fourier transform to make bootstrapped estimates of the error in UDs computationally feasible. Clustering and kernel smoothing identified 3 populations of polar bears in the region between Wrangel Island, Russia, and Banks Island, Canada. The relative probability of occurrence of animals from each population varied significantly among grid cells distributed across the study area. We displayed occurrence probabilities as contour maps wherein each contour line corresponded with a change in relative probability. Only at the edges of our study area and in some offshore regions were bootstrapped estimates of error in occurrence probabilities too high to allow prediction. Error estimates, which also were displayed as contours, allowed us to show that occurrence probabilities did not vary by season. Near Barrow, Alaska, 50% of bears observed are predicted to be from the Chukchi Sea population and 50% from the Southern Beaufort Sea population. At Tuktoyaktuk, Northwest Territories, Canada, 50% are from the Southern Beaufort Sea and 50% from the Northern Beaufort Sea population. The methods described here will aid managers of all wildlife that can be studied by telemetry to allocate harvests and other human perturbations to the appropriate populations, make risk assessments, and predict impacts of human activities. They will aid researchers by providing the refined descriptions of study populations that are necessary for population estimation and other investigative tasks. Arctic, Beaufort Sea, boundaries, clustering, Fourier transform, kernel, management, polar bears, population delineation, radiotelemetry, satellite, smoothing, Ursus maritimus

Application of the Modular Command and Control Structure (MCES) to Marine Corps SINCGARS Allocation

DTIC Science & Technology

1991-07-01

The first goal is to delineate the difference between the system being analyzed and its environment . To bound the C3 system, the analyst should...hardware and software entities and structures, is related to the forces it controls and the environmental stimuli to which it responds, including the enemy...M CES represents the environmental facto:s that require explicit assumptions in the problem. This ring may be seen as including the major scenario

Improved visual delineation of the intimal flap in Stanford type A and B dissections at 3rd generation dual-source high-pitch CT angiography.

PubMed

Beeres, Martin; Bucher, Andreas M; Wichmann, Julian L; Frellesen, Claudia; Scholtz, Jan E; Albrecht, Moritz; Bodelle, Boris; Nour-Eldin, Nour-Eldin A; Lee, Clara; Kaup, Moritz; Vogl, Thomas J; Gruber-Rouh, Tatjana

2016-07-01

Evaluation of the intimal flap visibility comparing 2nd and 3rd generation dual-source high-pitch CT. Twenty-five consecutive patients with aortic dissection underwent CT angiography on a second and third generation dual-source CT scanner using prospective ECG-gated high-pitch dual-source CT acquisition mode. Contrast material, saline flush and flow rate were kept equal for optimum comparability. The visibility of the intimal flap as well as the delineation of the different vascular structures was evaluated. In 3rd generation dual-source high-pitch CT we could show a significant improvement of intimal flap visibility in aortic dissection. Especially, the far end of the dissection membrane could be better evaluated in 3rd generation high-pitch CT, reaching statistical significance (P < 0.01). 3rd Generation high-pitch CT angiography shows a better delineation of the aortic intimal flap in a small patient cohort, especially in the far ends of the dissection membrane. This might be due to higher tube power in this CT generation. However, to generalise these findings larger trials are needed.

The population health record: concepts, definition, design, and implementation.

PubMed

Friedman, Daniel J; Parrish, R Gibson

2010-01-01

In 1997, the American Medical Informatics Association proposed a US information strategy that included a population health record (PopHR). Despite subsequent progress on the conceptualization, development, and implementation of electronic health records and personal health records, minimal progress has occurred on the PopHR. Adapting International Organization for Standarization electronic health records standards, we define the PopHR as a repository of statistics, measures, and indicators regarding the state of and influences on the health of a defined population, in computer processable form, stored and transmitted securely, and accessible by multiple authorized users. The PopHR is based upon an explicit population health framework and a standardized logical information model. PopHR purpose and uses, content and content sources, functionalities, business objectives, information architecture, and system architecture are described. Barriers to implementation and enabling factors and a three-stage implementation strategy are delineated.

Gaining Insight of Fetal Brain Development with Diffusion MRI and Histology

PubMed Central

Huang, Hao; Vasung, Lana

2013-01-01

Human brain is extraordinarily complex and yet its origin is a simple tubular structure. Its development during the fetal period is characterized by a series of accurately organized events which underlie the mechanisms of dramatic structural changes during fetal development. Revealing detailed anatomy at different stages of human fetal brain development provides insight on understanding not only this highly ordered process, but also the neurobiological foundations of cognitive brain disorders such as mental retardation, autism, schizophrenia, bipolar and language impairment. Diffusion tensor imaging (DTI) and histology are complementary tools which are capable of delineating the fetal brain structures at both macroscopic and microscopic level. In this review, the structural development of the fetal brains has been characterized with DTI and histology. Major components of the fetal brain, including cortical plate, fetal white matter and cerebral wall layer between the ventricle and subplate, have been delineated with DTI and histology. Anisotropic metrics derived from DTI were used to quantify the microstructural changes during the dynamic process of human fetal cortical development and prenatal development of other animal models. Fetal white matter pathways have been traced with DTI-based tractography to reveal growth patterns of individual white matter tracts and corticocortical connectivity. These detailed anatomical accounts of the structural changes during fetal period may provide the clues of detecting developmental and cognitive brain disorders at their early stages. The anatomical information from DTI and histology may also provide reference standards for diagnostic radiology of premature newborns. PMID:23796901

Gravimetry contributions to the study of the complex western Haouz aquifer (Morocco): Structural and hydrogeological implications

NASA Astrophysics Data System (ADS)

Chouikri, Ibtissam; el Mandour, Abdennabi; Jaffal, Mohammed; Baudron, Paul; García-Aróstegui, José-Luis; Manar, Ahmed; Casas, Albert

2016-03-01

This study provides new elements that illustrate the benefits of combining gravity, structural, stratigraphic and piezometric data for hydrogeological purposes. A combined methodology was applied to the western Haouz aquifer (Morocco), one of the main sources of water for irrigation and human consumption in the Marrakech region. First, a residual anomaly map was calculated from the Bouguer anomaly data. The computed map provided information on the ground density variation, revealing a strong control by a regional gradient. We then used various filtering techniques to delineate the major geological structures such as faults and basins: vertical and horizontal derivatives and upward continuation. This technique highlighted news structures and provided information on their dip. The gravity anomalies perfectly delineated the basement uplifts and the sedimentary thickening in depressions and grabens. The interpretation of gravimetric filtering, geological and hydrogeological data then highlighted two types of groundwater reservoirs, an unconfined aquifer hosted in conglomeratic mio-pliocene and quaternary rocks, covering the entire western Haouz and a deep confined aquifer contained in cenomanian-turonian limestone and eocene dolomitic formations in the south. Combining piezometric and residual anomaly maps revealed that groundwater flow and storage was in perfect agreement with the structures showing a negative anomaly, while structures with positive anomalies corresponded to groundwater divides. The study of gravity gradient zones by contact analysis enhanced the existing structural pattern of the study area and highlighted new structures, mainly oriented N70 and N130. The results of this study present a common framework and provide a notable step forward in the knowledge of the geometry and the groundwater flow pattern of the western Haouz aquifer, and will serve as a solid basis for a better water resource management.

Temporal and structural effects of stands on litter production in Melaleuca quinquenervia dominated wetlands of South Florida

USDA-ARS?s Scientific Manuscript database

Melaleuca quinquenervia (melaleuca) dominates large areas of the Florida Everglades in the southeastern USA where it has transformed sedge-dominated marshes into melaleuca forests. Despite its prevalence, very little is known about the ecology and stand dynamics of this invasive tree. We delineated...

Development of advanced acreage estimation methods

NASA Technical Reports Server (NTRS)

Guseman, L. F., Jr. (Principal Investigator)

1982-01-01

The development of an accurate and efficient algorithm for analyzing the structure of MSS data, the application of the Akaiki information criterion to mixture models, and a research plan to delineate some of the technical issues and associated tasks in the area of rice scene radiation characterization are discussed. The AMOEBA clustering algorithm is refined and documented.

Basic Concepts of CNS Development.

ERIC Educational Resources Information Center

Nowakowski, R. S.

1987-01-01

The goals of this review are to: (1) provide a set of concepts to aid in the understanding of complex processes which occur during central nervous system (CNS) development; (2) illustrate how they contribute to our knowlege of adult brain anatomy; and (3) delineate how modifications of normal developmental processes may affect the structure and…

"Thriving on Chaos." A Colloquium Review (Hagerstown, Maryland, May 18, 1988).

ERIC Educational Resources Information Center

Ernst, Charles M.; And Others

Three authors discuss Tom Peters' management guide, "Thriving on Chaos," and its implications for community colleges. First, Dixie D. LeHardy delineates Peters' main points concerning the U.S. economic system, the need for new organizational structures, the importance of quality in an unstable, competitive world market, methods of…

76 FR 24034 - Agency Information Collection Activities: Submission for OMB Review; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-29

... discussed at workgroup meetings. In turn, CMS' HCPCS workgroup reaches a decision as to whether a change... Level II Codes. As a result, the National Panel was delineated and CMS continued with the decision-making process under its current structure, the CMS HCPCS Workgroup (herein referred to as ``the...

A Computational Approach to Diagnosing Misfits, Inducing Requirements, and Delineating Transformations for Edge Organizations

DTIC Science & Technology

2005-06-01

of current military C2 organizations. The unit of analysis for organizational diagnosis is the Joint Task Force (JTF). It represents a multi-Service...Strategy as Structured Chaos Boston, MA: Harvard Business School Press (1998). [5] Burton, R.M. and Obel, B., Strategic Organizational Diagnosis and

Environmental Design Research. Volume One: Selected Papers. Community Development Series.

ERIC Educational Resources Information Center

Preiser, Wolfgang F. E., Ed.

The items contained in this volume are summaries and critiques of 43 research papers grouped within a framework of nine general topics which represents an attempt to delineate the basic concepts and structure of environmental design research. The papers are grouped under the following headings: (1) Theoretical issues in man-environment relations,…

Measuring ephemeral gully erosion rates and topographical thresholds in an urban watershed using unmanned aerial systems and structure-from-motion photogrammetric techniques

USDA-ARS?s Scientific Manuscript database

Rural and urban development can accelerate gully erosion, including on unpaved roads. Quantification of erosion from gullies is challenging in environments where gullies are rapidly repaired, and in urban areas where microtopographic complexity complicates delineation of contributing areas. This st...

Geologic map and map database of parts of Marin, San Francisco, Alameda, Contra Costa, and Sonoma counties, California

USGS Publications Warehouse

Blake, M.C.; Jones, D.L.; Graymer, R.W.; digital database by Soule, Adam

2000-01-01

This digital map database, compiled from previously published and unpublished data, and new mapping by the authors, represents the general distribution of bedrock and surficial deposits in the mapped area. Together with the accompanying text file (mageo.txt, mageo.pdf, or mageo.ps), it provides current information on the geologic structure and stratigraphy of the area covered. The database delineates map units that are identified by general age and lithology following the stratigraphic nomenclature of the U.S. Geological Survey. The scale of the source maps limits the spatial resolution (scale) of the database to 1:62,500 or smaller general distribution of bedrock and surficial deposits in the mapped area. Together with the accompanying text file (mageo.txt, mageo.pdf, or mageo.ps), it provides current information on the geologic structure and stratigraphy of the area covered. The database delineates map units that are identified by general age and lithology following the stratigraphic nomenclature of the U.S. Geological Survey. The scale of the source maps limits the spatial resolution (scale) of the database to 1:62,500 or smaller.

Application of geophysical techniques for 3D geohazard mapping to delineate cavities and potential sinkholes in the northern part of Kuala Lumpur, Malaysia.

PubMed

Bakhshipour, Zeinab; Huat, Bujang B K; Ibrahim, Shaharin; Asadi, Afshin; Kura, Nura Umar

2013-01-01

This work describes the application of the electrical resistivity (ER) method to delineating subsurface structures and cavities in Kuala Lumpur Limestone within the Batu Cave area of Selangor Darul Ehsan, Malaysia. In all, 17 ER profiles were measured by using a Wenner electrode configuration with 2 m spacing. The field survey was accompanied by laboratory work, which involves taking resistivity measurements of rock, soil, and water samples taken from the field to obtain the formation factor. The relationship between resistivity and the formation factor and porosity for all the samples was established. The porosity values were plotted and contoured. A 2-dimensional and 3-dimensional representation of the subsurface topography of the area was prepared through use of commercial computer software. The results show the presence of cavities and sinkholes in some parts of the study area. This work could help engineers and environmental managers by providing the information necessary to produce a sustainable management plan in order to prevent catastrophic collapses of structures and other related geohazard problems.

Homing in on sweet spots in Cretaceous Austin chalk

DOE Office of Scientific and Technical Information (OSTI.GOV)

Thomas, G.E.; Sonnenberg, F.P.

1993-11-29

In discussing the nature and causes of fracturing in the Cretaceous Austin chalk of south central Texas, many geologists and operators involved in horizontal drilling of the chalk consider regional rock stress as the probable main cause of the fractures. If Austin chalk fractures are mainly the result of regional extensional stress without localizing factors, then fractured sweet spots are randomly distributed and successful exploration is more or less a matter of luck, usually dependent upon the coincidental placement of a seismic line. But if local, deep-seated structure or basement topography are the main causes of sweet spots, then amore » successful exploration method would be to first delineate the basement paleo structure or topography and secondly, place a seismic line to confirm the delineated features. Finding localities of maximum fracturing and production would than be based on scientific logic rather than luck. It is the purpose of this article to present the results of an examination of these alternative causes for the Austin chalk fracturing in the hope of determining the most cost effective exploration method for the fractured chalk reservoir.« less

Application of Geophysical Techniques for 3D Geohazard Mapping to Delineate Cavities and Potential Sinkholes in the Northern Part of Kuala Lumpur, Malaysia

PubMed Central

Bakhshipour, Zeinab; Huat, Bujang B. K.; Ibrahim, Shaharin; Asadi, Afshin

2013-01-01

This work describes the application of the electrical resistivity (ER) method to delineating subsurface structures and cavities in Kuala Lumpur Limestone within the Batu Cave area of Selangor Darul Ehsan, Malaysia. In all, 17 ER profiles were measured by using a Wenner electrode configuration with 2 m spacing. The field survey was accompanied by laboratory work, which involves taking resistivity measurements of rock, soil, and water samples taken from the field to obtain the formation factor. The relationship between resistivity and the formation factor and porosity for all the samples was established. The porosity values were plotted and contoured. A 2-dimensional and 3-dimensional representation of the subsurface topography of the area was prepared through use of commercial computer software. The results show the presence of cavities and sinkholes in some parts of the study area. This work could help engineers and environmental managers by providing the information necessary to produce a sustainable management plan in order to prevent catastrophic collapses of structures and other related geohazard problems. PMID:24501583

Detailed photometric analysis of young star groups in the galaxy NGC 300

NASA Astrophysics Data System (ADS)

Rodríguez, M. J.; Baume, G.; Feinstein, C.

2016-10-01

Aims: The purpose of this work is to understand the global characteristics of the stellar populations in NGC 300. In particular, we focused our attention on searching young star groups and study their hierarchical organization. The proximity and orientation of this Sculptor Group galaxy make it an ideal candidate for this study. Methods: The research was conducted using archival point spread function (PSF) fitting photometry measured from images in multiple bands obtained with the Advanced Camera for Surveys of the Hubble Space Telescope (ACS/HST). Using the path linkage criterion (PLC), we cataloged young star groups and analyzed them from the observation of individual stars in the galaxy NGC 300. We also built stellar density maps from the bluest stars and applied the SExtractor code to identify overdensities. This method provided an additional tool for the detection of young stellar structures. By plotting isocontours over the density maps and comparing the two methods, we could infer and delineate the hierarchical structure of the blue population in the galaxy. For each region of a detected young star group, we estimated the size and derived the radial surface density profiles for stellar populations of different color (blue and red). A statistical decontamination of field stars was performed for each region. In this way it was possible to build the color-magnitude diagrams (CMD) and compare them with theoretical evolutionary models. We also constrained the present-day mass function (PDMF) per group by estimating a value for its slope. Results: The blue population distribution in NGC 300 clearly follows the spiral arms of the galaxy, showing a hierarchical behavior in which the larger and loosely distributed structures split into more compact and denser ones over several density levels. We created a catalog of 1147 young star groups in six fields of the galaxy NGC 300, in which we present their fundamental characteristics. The mean and the mode radius values obtained from the size distribution are both 25 pc, in agreement with the value for the Local Group and nearby galaxies. Additionally, we found an average PDMF slope that is compatible with the Salpeter value. Full Table 2 is only available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (http://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/594/A34

Transitions and Motivations for Substance Misuse in Prison Inmates With ADHD and Conduct Disorder: Validation of a New Instrument.

PubMed

Young, Susan; González, Rafael A; Wolff, Kim; Mutch, Laura; Malet-Lambert, Isabella; Gudjonsson, Gisli H

2017-01-01

There is a reasonable theoretical base for understanding the possible causes and motivations behind substance misuse and its dependency. There is a need for a reliable and valid measure that delineates the markers of substance use from its initiation and identifies different motivations for drug use transitioning, maintenance, and dependency. We addressed this gap in the United Kingdom by examining and validating the Substance Transitions in Addiction Rating Scale (STARS). For this review, 390 male prisoners were screened for conduct disorder and assessed with a clinical diagnostic interview for attention deficit/hyperactivity disorder (ADHD). They completed the four STARS subscales regarding their substance use. Exploratory structural equation modeling was performed to assess the STARS structure and to derive factors to assess validity against ADHD and conduct disorder diagnostic categories. Each of the subscales produced meaningful and reliable factors that supported the self-medication and behavioral disinhibition hypotheses of substance use motivation. The findings robustly show that ADHD is significantly associated with the need for coping as a way of managing primary and comorbid symptoms, but not conduct disorder. The findings were strongest for the combined ADHD type. STARS has a great potential to further the understanding of the motivation behind substance use and its dependency in different populations.

Parenting Deficits of Mothers Living with HIV/AIDS who have Young Children

PubMed Central

Murphy, Debra A.; Armistead, Lisa; Marelich, William D.; Herbeck, Diane M.

2014-01-01

The purpose of this study was to examine a model of the relationships between parenting deficits and skills, along with child outcomes, in a sample of mothers living with HIV (MLH) and their 6 to 14 year old children. Sixty-two MLH (61% Latina, 26% black, 3% white, & 10% multiracial) and their well children (age 6 – 14) were recruited from the greater Los Angeles, California, region to participate in an intervention (IMAGE: Improving Mothers’ parenting Abilities, Growth, and Effectiveness) designed to assist MLH with parenting and self-care skills. Constructs examined included parenting deficits, parenting skills, and child outcomes. Covariance structural modeling was used for the analyses. Covariance structural modeling confirmed the hypothesized set of construct associations. As predicted, fewer parenting deficits were associated with better parenting skills, which, in turn, were associated with better child outcomes. This study delineated further the parenting issues with which MLH struggle, providing information on the interventions needed for this population. MLH who have little confidence they can enact parenting skills and limited knowledge of basic parenting practices appear to be less likely to provide family routines consistently, monitor their children, or to engender family cohesion or a close parent-child relationship. Such parenting skills were found to be associated with child functioning. PMID:25620995

Cross-cultural confirmation of bi-factor models of a symptom distress measure: Symptom Checklist-90-Revised in clinical samples.

PubMed

Urbán, Róbert; Arrindell, Willem A; Demetrovics, Zsolt; Unoka, Zsolt; Timman, Reinier

2016-05-30

Four decades have elapsed since the introduction for clinical and research purposes of the Symptom Checklist-90(-R). Yet, its underlying dimensional structure has not been clearly delineated. A shift has been observed in the methods utilized-from predominantly exploratory factor analytic in nature in the first two decades or so to different confirmatory methods in recent years. A need remains to search for a structure that remains invariant across samples and nations. In that context, the present study attempted to replicate and extend recent findings yielded in a Hungarian general population sample (N=2,874) with two psychiatric patient samples from Hungary (N=972) and The Netherlands (N=1,902). In doing so, four models were contrasted: the one-factor model, Derogatis' nine factor model, a second-ordered factor model, and a bi-factor model. The bi-factor model was shown to yield the closest fit to the data in both countries. Further studies are needed to determine the stable number and kind of subscale scores that reflect the specific (primary) symptoms best, that is, those subscales with minimal shared variance with the overall general psychological distress dimension. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Genetic and Structure-Function Studies of Missense Mutations in Human Endothelial Lipase

PubMed Central

Razzaghi, Hamid; Tempczyk-Russell, Anna; Haubold, Kurt; Santorico, Stephanie A.; Shokati, Touraj; Christians, Uwe; Churchill, Mair E. A.

2013-01-01

Endothelial lipase (EL) plays a pivotal role in HDL metabolism. We sought to characterize EL and its interaction with HDL as well as its natural variants genetically, functionally and structurally. We screened our biethnic population sample (n = 802) for selected missense mutations (n = 5) and identified T111I as the only common variant. Multiple linear regression analyses in Hispanic subjects revealed an unexpected association between T111I and elevated LDL-C (p-value = 0.012) and total cholesterol (p-value = 0.004). We examined lipase activity of selected missense mutants (n = 10) and found different impacts on EL function, ranging from normal to complete loss of activity. EL-HDL lipidomic analyses indicated that EL has a defined remodeling of HDL without exhaustion of the substrate and a distinct and preference for several fatty acids that are lipid mediators and known for their potent pro- and anti-inflammatory properties. Structural studies using homology modeling revealed a novel α/β motif in the C-domain, unique to EL. The EL dimer was found to have the flexibility to expand and to bind various sizes of HDL particles. The likely impact of the all known missense mutations (n = 18) on the structure of EL was examined using molecular modeling and the impact they may have on EL lipase activity using a novel structure-function slope based on their structural free energy differences. The results of this multidisciplinary approach delineated the impact of EL and its variants on HDL. Moreover, the results suggested EL to have the capacity to modulate vascular health through its role in fatty acid-based signaling pathways. PMID:23536757

The genetical theory of social behaviour

PubMed Central

Lehmann, Laurent; Rousset, François

2014-01-01

We survey the population genetic basis of social evolution, using a logically consistent set of arguments to cover a wide range of biological scenarios. We start by reconsidering Hamilton's (Hamilton 1964 J. Theoret. Biol. 7, 1–16 (doi:10.1016/0022-5193(64)90038-4)) results for selection on a social trait under the assumptions of additive gene action, weak selection and constant environment and demography. This yields a prediction for the direction of allele frequency change in terms of phenotypic costs and benefits and genealogical concepts of relatedness, which holds for any frequency of the trait in the population, and provides the foundation for further developments and extensions. We then allow for any type of gene interaction within and between individuals, strong selection and fluctuating environments and demography, which may depend on the evolving trait itself. We reach three conclusions pertaining to selection on social behaviours under broad conditions. (i) Selection can be understood by focusing on a one-generation change in mean allele frequency, a computation which underpins the utility of reproductive value weights; (ii) in large populations under the assumptions of additive gene action and weak selection, this change is of constant sign for any allele frequency and is predicted by a phenotypic selection gradient; (iii) under the assumptions of trait substitution sequences, such phenotypic selection gradients suffice to characterize long-term multi-dimensional stochastic evolution, with almost no knowledge about the genetic details underlying the coevolving traits. Having such simple results about the effect of selection regardless of population structure and type of social interactions can help to delineate the common features of distinct biological processes. Finally, we clarify some persistent divergences within social evolution theory, with respect to exactness, synergies, maximization, dynamic sufficiency and the role of genetic arguments. PMID:24686929

Conservation genomics of anadromous Atlantic salmon across its North American range: outlier loci identify the same patterns of population structure as neutral loci.

PubMed

Moore, Jean-Sébastien; Bourret, Vincent; Dionne, Mélanie; Bradbury, Ian; O'Reilly, Patrick; Kent, Matthew; Chaput, Gérald; Bernatchez, Louis

2014-12-01

Anadromous Atlantic salmon (Salmo salar) is a species of major conservation and management concern in North America, where population abundance has been declining over the past 30 years. Effective conservation actions require the delineation of conservation units to appropriately reflect the spatial scale of intraspecific variation and local adaptation. Towards this goal, we used the most comprehensive genetic and genomic database for Atlantic salmon to date, covering the entire North American range of the species. The database included microsatellite data from 9142 individuals from 149 sampling locations and data from a medium-density SNP array providing genotypes for >3000 SNPs for 50 sampling locations. We used neutral and putatively selected loci to integrate adaptive information in the definition of conservation units. Bayesian clustering with the microsatellite data set and with neutral SNPs identified regional groupings largely consistent with previously published regional assessments. The use of outlier SNPs did not result in major differences in the regional groupings, suggesting that neutral markers can reflect the geographic scale of local adaptation despite not being under selection. We also performed assignment tests to compare power obtained from microsatellites, neutral SNPs and outlier SNPs. Using SNP data substantially improved power compared to microsatellites, and an assignment success of 97% to the population of origin and of 100% to the region of origin was achieved when all SNP loci were used. Using outlier SNPs only resulted in minor improvements to assignment success to the population of origin but improved regional assignment. We discuss the implications of these new genetic resources for the conservation and management of Atlantic salmon in North America. © 2014 John Wiley & Sons Ltd.

The genetical theory of social behaviour.

PubMed

Lehmann, Laurent; Rousset, François

2014-05-19

We survey the population genetic basis of social evolution, using a logically consistent set of arguments to cover a wide range of biological scenarios. We start by reconsidering Hamilton's (Hamilton 1964 J. Theoret. Biol. 7, 1-16 (doi:10.1016/0022-5193(64)90038-4)) results for selection on a social trait under the assumptions of additive gene action, weak selection and constant environment and demography. This yields a prediction for the direction of allele frequency change in terms of phenotypic costs and benefits and genealogical concepts of relatedness, which holds for any frequency of the trait in the population, and provides the foundation for further developments and extensions. We then allow for any type of gene interaction within and between individuals, strong selection and fluctuating environments and demography, which may depend on the evolving trait itself. We reach three conclusions pertaining to selection on social behaviours under broad conditions. (i) Selection can be understood by focusing on a one-generation change in mean allele frequency, a computation which underpins the utility of reproductive value weights; (ii) in large populations under the assumptions of additive gene action and weak selection, this change is of constant sign for any allele frequency and is predicted by a phenotypic selection gradient; (iii) under the assumptions of trait substitution sequences, such phenotypic selection gradients suffice to characterize long-term multi-dimensional stochastic evolution, with almost no knowledge about the genetic details underlying the coevolving traits. Having such simple results about the effect of selection regardless of population structure and type of social interactions can help to delineate the common features of distinct biological processes. Finally, we clarify some persistent divergences within social evolution theory, with respect to exactness, synergies, maximization, dynamic sufficiency and the role of genetic arguments.

Putaminal volume and diffusion in early familial Creutzfeldt-Jakob disease.

PubMed

Seror, Ilana; Lee, Hedok; Cohen, Oren S; Hoffmann, Chen; Prohovnik, Isak

2010-01-15

The putamen is centrally implicated in the pathophysiology of Creutzfeldt-Jakob Disease (CJD). To our knowledge, its volume has never been measured in this disease. We investigated whether gross putaminal atrophy can be detected by MRI in early stages, when the diffusion is already reduced. Twelve familial CJD patients with the E200K mutation and 22 healthy controls underwent structural and diffusion MRI scans. The putamen was identified in anatomical scans by two methods: manual tracing by a blinded investigator, and automatic parcellation by a computerized segmentation procedure (FSL FIRST). For each method, volume and mean Apparent Diffusion Coefficient (ADC) were calculated. ADC was significantly lower in CJD patients (697+/-64 microm(2)/s vs. 750+/-31 microm(2)/s, p<0.005), as expected, but the volume was not reduced. The computerized FIRST delineation yielded comparable ADC values to the manual method, but computerized volumes were smaller than manual tracing values. We conclude that significant diffusion reduction in the putamen can be detected by delineating the structure manually or with a computerized algorithm. Our findings confirm and extend previous voxel-based and observational studies. Putaminal volume was not reduced in our early-stage patients, thus confirming that diffusion abnormalities precede detectible atrophy in this structure.

Delineation of tectonic provinces of New York state as a component of seismic-hazard evaluation

USGS Publications Warehouse

Fakundiny, R.H.

2004-01-01

Seismic-hazard evaluations in the eastern United States must be based on interpretations of the composition and form of Proterozoic basement-rock terranes and overlying Paleozoic strata, and on factors that can cause relative movements among their units, rather than Phanerozoic orogenic structures, which may be independent of modern tectonics. The tectonic-province concept is a major part of both probabilistic and deterministic seismic-hazard evaluations, yet those that have been proposed to date have not attempted to geographically correlate modern earthquakes with regional basement structure. Comparison of basement terrane (megablock) boundaries with the spatial pattern of modern seismicity may lead to the mechanically sound definition of tectonic provinces, and thus, better seismic-hazard evaluation capability than is currently available. Delineation of megablock boundaries will require research on the many factors that affect their structure and movement. This paper discusses and groups these factors into two broad categories-megablock tectonics in relation to seismicity and regional horizontal-compressive stresses, with megablock tectonics divided into subcategories of basement, overlying strata, regional lineaments, basement tectonic terranes, earthquake epicenter distribution, and epeirogeny, and compressive stresses divided into pop-ups and the contemporary maximum horizontal-compressive stress field. A list presenting four to nine proposed research topics for each of these categories is given at the end.

Joint inversion of seismic and gravity data for imaging seismic velocity structure of the crust and upper mantle beneath Utah, United States

DOE PAGES

Syracuse, Ellen Marie; Zhang, Haijiang; Maceira, Monica

2017-07-11

Here, we present a method for using any combination of body wave arrival time measurements, surface wave dispersion observations, and gravity data to simultaneously invert for three-dimensional P- and S-wave velocity models. The simultaneous use of disparate data types takes advantage of the differing sensitivities of each data type, resulting in a comprehensive and higher resolution three-dimensional geophysical model. In a case study for Utah, we combine body waves first arrivals mainly from the USArray Transportable Array, Rayleigh wave group and phase velocity dispersion data, and Bouguer gravity anomalies to invert for crustal and upper mantle structure of the region.more » Results show clear delineations, visible in both P- and S-wave velocities, between the three main tectonic provinces in the region. In conclusion, without the inclusion of the surface wave and gravity constraints, these delineations are less clear, particularly for S-wave velocities. Indeed, checkerboard tests confirm that the inclusion of the additional datasets dramatically improves S-wave velocity recovery, with more subtle improvements to P-wave velocity recovery, demonstrating the strength of the method in successfully recovering seismic velocity structure from multiple types of constraints.« less

Joint inversion of seismic and gravity data for imaging seismic velocity structure of the crust and upper mantle beneath Utah, United States

DOE Office of Scientific and Technical Information (OSTI.GOV)

Syracuse, Ellen Marie; Zhang, Haijiang; Maceira, Monica

Here, we present a method for using any combination of body wave arrival time measurements, surface wave dispersion observations, and gravity data to simultaneously invert for three-dimensional P- and S-wave velocity models. The simultaneous use of disparate data types takes advantage of the differing sensitivities of each data type, resulting in a comprehensive and higher resolution three-dimensional geophysical model. In a case study for Utah, we combine body waves first arrivals mainly from the USArray Transportable Array, Rayleigh wave group and phase velocity dispersion data, and Bouguer gravity anomalies to invert for crustal and upper mantle structure of the region.more » Results show clear delineations, visible in both P- and S-wave velocities, between the three main tectonic provinces in the region. In conclusion, without the inclusion of the surface wave and gravity constraints, these delineations are less clear, particularly for S-wave velocities. Indeed, checkerboard tests confirm that the inclusion of the additional datasets dramatically improves S-wave velocity recovery, with more subtle improvements to P-wave velocity recovery, demonstrating the strength of the method in successfully recovering seismic velocity structure from multiple types of constraints.« less

Tectonics and crustal structure of the Saurashtra peninsula: based on Gravity and Magnetic data

NASA Astrophysics Data System (ADS)

Mishra, A. K.; Singh, A.; Singh, U. K.

2016-12-01

The Saurashtra peninsula is located at the North Western margin of the Indian shield which occurs as a horst block between the rifts namely as Kachchh, Cambay and Narmada. It is important because of occurrence of moderate earthquake and presence of mesozoic sediments below the Deccan trap. The maps of bouguer gravity anomaly and the total intensity magnetic anomalies of Saurashtra have delineated six circular gravity highs of magnitudes 40-60 mGal and 800-1000 nT respectively. In order to understand the location, structure and depth of the source body, methods like continuous wavelet transform (CWT), Euler deconvolution and power spectrum analysis have been implemented in the potential field data. The CWT and Euler deconvolution give 16-18 km average depth of volcanic plug in Junagadh and Rajula region. From the power spectrum analysis, it is found that average Moho depth in the Saurashtra is about 36-38 km. Keeping the constraints obtained from geophysical studies like borehole, deep seismic survey, receiver function analysis and geological information, combined gravity and magnetic modeling have been performed. Detailed crustal structure of the Saurashtra region has been delineated along two profiles which pass from prominent geological features Junagadh and Rajula volcanic plugs respectively.

Flat-panel-detector chest radiography: effect of tube voltage on image quality.

PubMed

Uffmann, Martin; Neitzel, Ulrich; Prokop, Mathias; Kabalan, Nahla; Weber, Michael; Herold, Christian J; Schaefer-Prokop, Cornelia

2005-05-01

To compare the visibility of anatomic structures in direct-detector chest radiographs acquired with different tube voltages at equal effective doses to the patient. The study protocol was approved by the institutional internal review board, and written informed consent was obtained from all patients. Posteroanterior chest radiographs of 48 consecutively selected patients were obtained at 90, 121, and 150 kVp by using a flat-panel-detector unit that was based on cesium iodide technology and automated exposure control. Monte Carlo simulations were used to verify that the effective dose for all kilovoltage settings was equal. Five radiologists subjectively and independently rated the delineation of anatomic structures on hard-copy images by using a five-point scale. They also ranked image quality in a blinded side-by-side comparison. Average ranking scores were compared by using one-way analysis of variance with repeated measures. Data were analyzed for the entire patient group and for two patient subgroups that were formed according to body mass index (BMI). The visibility scores of most anatomic structures were significantly superior with the 90-kVp images (mean score, 3.11), followed by the 121-kVp (mean score, 2.95) and 150-kVp images (mean score, 2.80). Differences did not reach significance (P > .05) only for the delineation of the peripheral vessels, the heart contours, and the carina. This was also true for the subgroup of patients (n = 24) with a BMI greater than and the subgroup of patients (n = 24) with a BMI less than the mean BMI (26.9 kg/m(2)). At side-by-side comparison, the readers rated 90-kVp images as having superior image quality in the majority of image triplets; the percentage of 90-kVp images rated as "first choice" ranged from 60% (29 of 48 patients) to 90% (43 of 48 patients), with a median of 88% (42 of 48 patients), among the readers. Delineation of most anatomic structures and overall image quality were ranked superior in digital radiographs acquired with lower kilovoltage at a constant effective patient dose. (c) RSNA, 2005.

New York City, Hudson River, NY, USA

NASA Technical Reports Server (NTRS)

1991-01-01

This color infrared photo of New York City, Hudson River, NY, (41.0N, 74.0W) shows a unique view of the dense urban development of the New York City metropolitan area in downstate New York, Long Island and New Jersey. The heavily populated city areas appear as white or gray while vegetated areas appear as shades of red. Central park clearly shows up on Manhattan Island as an illustration of the delineation between cultural and natural features.

Water Stress on U.S. Power Production at Decadal Time Horizons

NASA Astrophysics Data System (ADS)

Ganguli, P.; Kumar, D.; Yun, J.; Short, G.; Klausner, J.; Ganguly, A. R.

2014-12-01

Thermoelectric power production at risk, owing to current and projected water scarcity and rising stream temperatures, is assessed for the continental United States (US) at decadal scales. Regional water scarcity is driven by climate variability and change, as well as by multi-sector water demand. While a planning horizon of zero to about thirty years is occasionally prescribed by stakeholders, the challenges to risk assessment at these scales include the difficulty in delineating decadal climate trends from intrinsic natural or multiple model variability. Current generation global climate or earth system models are not credible at the spatial resolutions of power plants, especially for surface water quantity and stream temperatures, which further exacerbates the assessment challenge. Population changes, which are anyway difficult to project, cannot serve as adequate proxies for changes in the water demand across sectors. The hypothesis that robust assessments of power production at risks are possible, despite the uncertainties, has been examined as a proof of concept. An approach is presented for delineating water scarcity and temperature from climate models, observations and population storylines, as well as for assessing power production at risk by examining geospatial correlations of power plant locations within regions where the usable water supply for energy production happens to be scarcer and warmer. Acknowledgment: Funding provided by US DOE's ARPA-E through Award DE-AR0000374.

Effective surveillance strategies following a potential classical Swine Fever incursion in a remote wild pig population in North-Western Australia.

PubMed

Leslie, E; Cowled, B; Graeme Garner, M; Toribio, J-A L M L; Ward, M P

2014-10-01

Early disease detection and efficient methods of proving disease freedom can substantially improve the response to incursions of important transboundary animal diseases in previously free regions. We used a spatially explicit, stochastic disease spread model to simulate the spread of classical swine fever in wild pigs in a remote region of northern Australia and to assess the performance of disease surveillance strategies to detect infection at different time points and to delineate the size of the resulting outbreak. Although disease would likely be detected, simple random sampling was suboptimal. Radial and leapfrog sampling improved the effectiveness of surveillance at various stages of the simulated disease incursion. This work indicates that at earlier stages, radial sampling can reduce epidemic length and achieve faster outbreak delineation and control, but at later stages leapfrog sampling will outperform radial sampling in relation to supporting faster disease control with a less-extensive outbreak area. Due to the complexity of wildlife population dynamics and group behaviour, a targeted approach to surveillance needs to be implemented for the efficient use of resources and time. Using a more situation-based surveillance approach and accounting for disease distribution and the time period over which an epidemic has occurred is the best way to approach the selection of an appropriate surveillance strategy. © 2013 Blackwell Verlag GmbH.

Procedures for Delineating and Characterizing Watersheds for Stream and River Monitoring Programs (Final Report)

EPA Science Inventory

EPA has released the document, Procedures for Delineating and Characterizing Watersheds for Stream and River Monitoring Programs (EPA/600/R-17/448F). This manual describes how states and tribes can delineate and characterize watersheds. It explains how to delineate water...

The significance of cultural patterning for the "alcoholic family".

PubMed

Ablon, J

1980-06-01

This paper presents findings from a recent study of a population of middle-class Catholic families, primarily Irish, German, and Italian, among whom alcohol-related problems are frequent and severe. To understand alcohol usage in this population, a knowledge of the historical and cultural roles of drinking in the relevant ethnic or national groups and a holistic view of contemporary family life are essential. It is suggested that massive social controls in major areas of family life are closely related to problematic drinking behavior. The delineation of cultural prescriptions regarding behaviors and attitudes directly and indirectly related to drinking patterns may contribute a significant cultural dimension to proposed models of the "alcoholic family" system.

Fixed points and limit cycles in the population dynamics of lysogenic viruses and their hosts

NASA Astrophysics Data System (ADS)

Wang, Zhenyu; Goldenfeld, Nigel

2010-07-01

Starting with stochastic rate equations for the fundamental interactions between microbes and their viruses, we derive a mean-field theory for the population dynamics of microbe-virus systems, including the effects of lysogeny. In the absence of lysogeny, our model is a generalization of that proposed phenomenologically by Weitz and Dushoff. In the presence of lysogeny, we analyze the possible states of the system, identifying a limit cycle, which we interpret physically. To test the robustness of our mean-field calculations to demographic fluctuations, we have compared our results with stochastic simulations using the Gillespie algorithm. Finally, we estimate the range of parameters that delineate the various steady states of our model.

Apport de la gravimétrie à l'étude de la structure du bassin des Triffa (Maroc nord-oriental) : implications hydrogéologiques

NASA Astrophysics Data System (ADS)

Khattach, Driss; Keating, Pierre; Mili, El Mostafa; Chennouf, Touria; Andrieux, Pierre; Milhi, Abdellah

2004-12-01

Various techniques (horizontal and vertical derivatives, upward continuation, Euler deconvolution) have been applied to the gravity data from the Triffa's plain and the north flank of the Beni-Snassen massif to delineate various major geological structures such as faults and basins. These results allow the production of a structural map showing the fault systems for the survey area. This map forms the basis for planning future hydrogeological research in this region. To cite this article: D. Khattach et al., C. R. Geoscience 336 (2004).

Teaching/learning strategies for the essentials of baccalaureate nursing education for entry-level community/public health nursing.

PubMed

Callen, Bonnie; Smith, Claudia M; Joyce, Barbara; Lutz, Jayne; Brown-Schott, Nancy; Block, Derryl

2013-01-01

The purpose of this article is to describe teaching/learning strategies for each of the 15 Essentials of Baccalaureate Nursing Education for Entry-Level Community/Public Health Nursing (ACHNE, 2009). Carper's ways of knowing serve as foundations for creating classroom and clinical experiences that focus on clinical action with community as client. Each community/public health essential is defined with relevance to community/public health nursing practice. Five teaching/learning strategies have been delineated for each essential with suggestions of teaching resources and/or target population application. Teaching/learning strategies that focus on community as client, population health, and the essential knowledge and competencies of C/PH nursing will help ensure preparation of baccalaureate prepared nurses with knowledge and skills to improve the health of populations. © 2013 Wiley Periodicals, Inc.

The heterogeneity of human CD127(+) innate lymphoid cells revealed by single-cell RNA sequencing.

PubMed

Björklund, Åsa K; Forkel, Marianne; Picelli, Simone; Konya, Viktoria; Theorell, Jakob; Friberg, Danielle; Sandberg, Rickard; Mjösberg, Jenny

2016-04-01

Innate lymphoid cells (ILCs) are increasingly appreciated as important participants in homeostasis and inflammation. Substantial plasticity and heterogeneity among ILC populations have been reported. Here we have delineated the heterogeneity of human ILCs through single-cell RNA sequencing of several hundreds of individual tonsil CD127(+) ILCs and natural killer (NK) cells. Unbiased transcriptional clustering revealed four distinct populations, corresponding to ILC1 cells, ILC2 cells, ILC3 cells and NK cells, with their respective transcriptomes recapitulating known as well as unknown transcriptional profiles. The single-cell resolution additionally divulged three transcriptionally and functionally diverse subpopulations of ILC3 cells. Our systematic comparison of single-cell transcriptional variation within and between ILC populations provides new insight into ILC biology during homeostasis, with additional implications for dysregulation of the immune system.

Unified Program Design: Organizing Existing Programming Models, Delivery Options, and Curriculum

ERIC Educational Resources Information Center

Rubenstein, Lisa DaVia; Ridgley, Lisa M.

2017-01-01

A persistent problem in the field of gifted education has been the lack of categorization and delineation of gifted programming options. To address this issue, we propose Unified Program Design as a structural framework for gifted program models. This framework defines gifted programs as the combination of delivery methods and curriculum models.…

Cost Study of Educational Media Systems and Their Equipment Components. Volume II, Technical Report. Final Report.

ERIC Educational Resources Information Center

General Learning Corp., Washington, DC.

A common instructional task and a set of educational environments are hypothesized for analysis of media cost data. The analytic structure may be conceptulized as a three-dimensional matrix: the first vector separates costs into production, distribution, and reception; the second vector delineates capital (initial) and operating (annual) costs;…

Mineral deposits in western Saudi Arabia; a preliminary report

USGS Publications Warehouse

Roberts, Ralph Jackson; Greenwood, William R.; Worl, Ronald G.; Dodge, F.C.W.; Kiilsgaard, Thor H.

1975-01-01

In order to effectively carry on a search for new mineral deposits, the belts should be mapped in detail, with emphasis on the delineation of stratigraphic and structural features that control metallization. In addition, geochemical and geophysical studies should be made of promising areas to outline exploration targets. These targets could then be systematically explored.

Mission 1980--and Beyond--Annual Report of the American Association of Community and Junior Colleges.

ERIC Educational Resources Information Center

Community and Junior College Journal, 1981

1981-01-01

Presents the newly adopted mission statement of the American Association of Community and Junior Colleges (AACJC). Describes how the programs initiated during 1980, AACJC's interaction with the federal government, and its public relations efforts served to advance this mission. Delineates AACJC's structure and the roles of various units. (DML)

Nursing Minimum Data Set for School Nursing Practice. Position Statement. Revised

ERIC Educational Resources Information Center

Denehy, Janice

2012-01-01

It is the position of the National Association of School Nurses (NASN) to support the collection of essential nursing data as listed in the Nursing Minimum Data Set (NMDS). The NMDS provides a basic structure to identify the data needed to delineate nursing care delivered to clients as well as relevant characteristics of those clients. Structure…

Using Educational Technology to Mediate Informal, Task-Conscious Learning: Design Innovations in Two European Projects

ERIC Educational Resources Information Center

Cook, John

2012-01-01

The purpose of this article is to feature two European projects that have explored innovative approaches to using educational technology to mediate "informal learning" in a variety of contexts. The article is structured as follows: Firstly, it briefly delineates what the author means by "informal learning," opting for the term…

The Bridges SOI Model School Program at Palo Verde School, Palo Verde, Arizona.

ERIC Educational Resources Information Center

Stock, William A.; DiSalvo, Pamela M.

The Bridges SOI Model School Program is an educational service based upon the SOI (Structure of Intellect) Model School curriculum. For the middle seven months of the academic year, all students in the program complete brief daily exercises that develop specific cognitive skills delineated in the SOI model. Additionally, intensive individual…

School Education and Development of Gender Perspectives and Sexuality in Japan

ERIC Educational Resources Information Center

Hashimoto, Noriko; Ushitora, Kaori; Morioka, Mari; Motegi, Terunori; Tanaka, Kazue; Tashiro, Mieko; Inoue, Emiko; Ikeya, Hisao; Sekiguchi, Hisashi; Marui, Yoshimi; Sawamura, Fumika

2017-01-01

This study aimed to evaluate changes in the attitudes of Japan's post-war youth towards gender equality and sexuality, and to examine whether these attitudes bore a relationship to school education. Different generations were delineated based on changes in courses of study and year of birth, and semi-structured interviews were conducted enquiring…

Long-Term Exercise Is Needed to Enhance Synaptic Plasticity in the Hippocampus

ERIC Educational Resources Information Center

Patten, Anna R.; Sickmann, Helle; Hryciw, Brett N.; Kucharsky, Tessa; Parton, Roberta; Kernick, Aimee; Christie, Brian R.

2013-01-01

Exercise can have many benefits for the body, but it also benefits the brain by increasing neurogenesis, synaptic plasticity, and performance on learning and memory tasks. The period of exercise needed to realize the structural and functional benefits for the brain have not been well delineated, and previous studies have used periods of exercise…

Curriculum Choices and School-To-Work Transitions among Upper-Secondary School Leavers in Scotland and Ireland

ERIC Educational Resources Information Center

Iannelli, Cristina; Smyth, Emer

2017-01-01

David Raffe was a highly influential figure in the field of transitions research. His work carefully delineated how national institutional policies shape transition processes and outcomes. Curriculum structure and organisation were seen as key features of these transition systems, his work tracing the relative impact of vocational and academic…

An efficient algorithm for generating diverse microstructure sets and delineating properties closures

DOE PAGES

Johnson, Oliver K.; Kurniawan, Christian

2018-02-03

Properties closures delineate the theoretical objective space for materials design problems, allowing designers to make informed trade-offs between competing constraints and target properties. In this paper, we present a new algorithm called hierarchical simplex sampling (HSS) that approximates properties closures more efficiently and faithfully than traditional optimization based approaches. By construction, HSS generates samples of microstructure statistics that span the corresponding microstructure hull. As a result, we also find that HSS can be coupled with synthetic polycrystal generation software to generate diverse sets of microstructures for subsequent mesoscale simulations. Finally, by more broadly sampling the space of possible microstructures, itmore » is anticipated that such diverse microstructure sets will expand our understanding of the influence of microstructure on macroscale effective properties and inform the construction of higher-fidelity mesoscale structure-property models.« less

Ultrastructural aspects of feeding and secretion-excretion by the equine parasite Strongylus vulgaris.

PubMed

Mobarak, M S; Ryan, M F

1999-06-01

Light, scanning, and transmission electron microscopy were employed to provide further data on the putative origins of the immunogenic secretory-excretory product (ESP) of Strongylus vulgaris (Looss 1900). The sharply delineated but superficial attachment to the equine caecum by the mouth leaves behind an oval area devoid of epithelial cells. Attachment does not extend deeply enough to reach the muscularis mucosa layer of the equine intestine. The progressive digestion of the ingested plug of tissue (epithelial cells, blood cells and mucous) was visualized. The coelomocytes, floating cells and membranous structures located in the pseudocoelom and intimately associated with the digestive, excretory and reproductive systems, and with the somatic muscles are described. The secretory-excretory system comprises two, ventrally-located, secretory-excretory glands connected to tubular elements. These glands synthesize granules of various sizes and densities that are delineated.

An efficient algorithm for generating diverse microstructure sets and delineating properties closures

DOE Office of Scientific and Technical Information (OSTI.GOV)

Johnson, Oliver K.; Kurniawan, Christian

Properties closures delineate the theoretical objective space for materials design problems, allowing designers to make informed trade-offs between competing constraints and target properties. In this paper, we present a new algorithm called hierarchical simplex sampling (HSS) that approximates properties closures more efficiently and faithfully than traditional optimization based approaches. By construction, HSS generates samples of microstructure statistics that span the corresponding microstructure hull. As a result, we also find that HSS can be coupled with synthetic polycrystal generation software to generate diverse sets of microstructures for subsequent mesoscale simulations. Finally, by more broadly sampling the space of possible microstructures, itmore » is anticipated that such diverse microstructure sets will expand our understanding of the influence of microstructure on macroscale effective properties and inform the construction of higher-fidelity mesoscale structure-property models.« less

Clinical evaluation of multi-atlas based segmentation of lymph node regions in head and neck and prostate cancer patients.

PubMed

Sjöberg, Carl; Lundmark, Martin; Granberg, Christoffer; Johansson, Silvia; Ahnesjö, Anders; Montelius, Anders

2013-10-03

Semi-automated segmentation using deformable registration of selected atlas cases consisting of expert segmented patient images has been proposed to facilitate the delineation of lymph node regions for three-dimensional conformal and intensity-modulated radiotherapy planning of head and neck and prostate tumours. Our aim is to investigate if fusion of multiple atlases will lead to clinical workload reductions and more accurate segmentation proposals compared to the use of a single atlas segmentation, due to a more complete representation of the anatomical variations. Atlases for lymph node regions were constructed using 11 head and neck patients and 15 prostate patients based on published recommendations for segmentations. A commercial registration software (Velocity AI) was used to create individual segmentations through deformable registration. Ten head and neck patients, and ten prostate patients, all different from the atlas patients, were randomly chosen for the study from retrospective data. Each patient was first delineated three times, (a) manually by a radiation oncologist, (b) automatically using a single atlas segmentation proposal from a chosen atlas and (c) automatically by fusing the atlas proposals from all cases in the database using the probabilistic weighting fusion algorithm. In a subsequent step a radiation oncologist corrected the segmentation proposals achieved from step (b) and (c) without using the result from method (a) as reference. The time spent for editing the segmentations was recorded separately for each method and for each individual structure. Finally, the Dice Similarity Coefficient and the volume of the structures were used to evaluate the similarity between the structures delineated with the different methods. For the single atlas method, the time reduction compared to manual segmentation was 29% and 23% for head and neck and pelvis lymph nodes, respectively, while editing the fused atlas proposal resulted in time reductions of 49% and 34%. The average volume of the fused atlas proposals was only 74% of the manual segmentation for the head and neck cases and 82% for the prostate cases due to a blurring effect from the fusion process. After editing of the proposals the resulting volume differences were no longer statistically significant, although a slight influence by the proposals could be noticed since the average edited volume was still slightly smaller than the manual segmentation, 9% and 5%, respectively. Segmentation based on fusion of multiple atlases reduces the time needed for delineation of lymph node regions compared to the use of a single atlas segmentation. Even though the time saving is large, the quality of the segmentation is maintained compared to manual segmentation.

A Definitive Optical Detection of a Supercluster at Z ~ 0.91

NASA Astrophysics Data System (ADS)

Lubin, Lori M.; Brunner, Robert; Metzger, Mark R.; Postman, Marc; Oke, J. B.

2000-03-01

We present the results from a multiband optical imaging program that has definitively confirmed the existence of a supercluster at z~0.91. Two massive clusters of galaxies, Cl 1604+4304 at z=0.897 and Cl 1604+4321 at z=0.924, were originally observed in the high-redshift cluster survey of Oke, Postman, & Lubin. They are separated by 4300 km s-1 in radial velocity and 17' on the plane of the sky. Their physical and redshift proximity suggested a promising supercluster candidate. Deep BRi imaging of the region between the two clusters indicates a large population of red galaxies. This population forms a tight, red sequence in the color-magnitude diagram at (R-i)~1.4. The characteristic color is identical to that of the spectroscopically confirmed early-type galaxies in the two member clusters. The red galaxies are spread throughout the 5 h-1 Mpc region between Cl 1604+4304 and Cl 1604+4321. Their spatial distribution delineates the entire large-scale structure with high concentrations at the cluster centers. In addition, we detect a significant overdensity of red galaxies directly between Cl 1604+4304 and Cl 1604+4321 which is the signature of a third, rich cluster associated with this system. The strong sequence of red galaxies and their spatial distribution clearly indicate that we have discovered a supercluster at z~0.91.

Northern perspectives on medical elective tourism: a qualitative study.

PubMed

Coke, Sarah; Kuper, Ayelet; Richardson, Lisa; Cameron, Anita

2016-01-01

The Royal College of Physicians and Surgeons of Canada recognizes education to be necessary for doctors to provide culturally safe care. Communities in northern Canada have large populations of Aboriginal people and other marginalized groups. Our goal was to identify the elements of appropriate predeparture curricula for these medical trainees. We conducted our study in Kenora, Ontario. With the help of a core collaborative group and the support of the local Aboriginal Health Access Centre, we interviewed a purposive sample of community members about their interactions with trainees from southern Canada. Aboriginal and non-Aboriginal researchers with roots in northern and southern Canada brought perspectives to the inductive analysis. We conducted 17 semistructured interviews between February and March 2014. Participants felt that southern trainees were inadequately educated in northern politics, society and history. They identified 2 more themes: determinants of health affecting the local Aboriginal population, and provider and patient factors affecting delivery of culturally competent care. Participants also shared ideas on how best to implement this content into curricula. Providing culturally competent care to northern communities is a complex process requiring education. Using a collaborative method, we were able to delineate the experiences of members of a northern community and identify knowledge gaps of southern trainees travelling there. Our results provide a foundation for the content and structure of formal predeparture curricula to enable such trainees to provide culturally safe care.

Advances in Setaria genomics for genetic improvement of cereals and bioenergy grasses.

PubMed

Muthamilarasan, Mehanathan; Prasad, Manoj

2015-01-01

Recent advances in Setaria genomics appear promising for genetic improvement of cereals and biofuel crops towards providing multiple securities to the steadily increasing global population. The prominent attributes of foxtail millet (Setaria italica, cultivated) and green foxtail (S. viridis, wild) including small genome size, short life-cycle, in-breeding nature, genetic close-relatedness to several cereals, millets and bioenergy grasses, and potential abiotic stress tolerance have accentuated these two Setaria species as novel model system for studying C4 photosynthesis, stress biology and biofuel traits. Considering this, studies have been performed on structural and functional genomics of these plants to develop genetic and genomic resources, and to delineate the physiology and molecular biology of stress tolerance, for the improvement of millets, cereals and bioenergy grasses. The release of foxtail millet genome sequence has provided a new dimension to Setaria genomics, resulting in large-scale development of genetic and genomic tools, construction of informative databases, and genome-wide association and functional genomic studies. In this context, this review discusses the advancements made in Setaria genomics, which have generated a considerable knowledge that could be used for the improvement of millets, cereals and biofuel crops. Further, this review also shows the nutritional potential of foxtail millet in providing health benefits to global population and provides a preliminary information on introgressing the nutritional properties in graminaceous species through molecular breeding and transgene-based approaches.

Atlas-based automatic segmentation of head and neck organs at risk and nodal target volumes: a clinical validation.

PubMed

Daisne, Jean-François; Blumhofer, Andreas

2013-06-26

Intensity modulated radiotherapy for head and neck cancer necessitates accurate definition of organs at risk (OAR) and clinical target volumes (CTV). This crucial step is time consuming and prone to inter- and intra-observer variations. Automatic segmentation by atlas deformable registration may help to reduce time and variations. We aim to test a new commercial atlas algorithm for automatic segmentation of OAR and CTV in both ideal and clinical conditions. The updated Brainlab automatic head and neck atlas segmentation was tested on 20 patients: 10 cN0-stages (ideal population) and 10 unselected N-stages (clinical population). Following manual delineation of OAR and CTV, automatic segmentation of the same set of structures was performed and afterwards manually corrected. Dice Similarity Coefficient (DSC), Average Surface Distance (ASD) and Maximal Surface Distance (MSD) were calculated for "manual to automatic" and "manual to corrected" volumes comparisons. In both groups, automatic segmentation saved about 40% of the corresponding manual segmentation time. This effect was more pronounced for OAR than for CTV. The edition of the automatically obtained contours significantly improved DSC, ASD and MSD. Large distortions of normal anatomy or lack of iodine contrast were the limiting factors. The updated Brainlab atlas-based automatic segmentation tool for head and neck Cancer patients is timesaving but still necessitates review and corrections by an expert.

Northern perspectives on medical elective tourism: a qualitative study

PubMed Central

Coke, Sarah; Kuper, Ayelet; Richardson, Lisa; Cameron, Anita

2016-01-01

Background: The Royal College of Physicians and Surgeons of Canada recognizes education to be necessary for doctors to provide culturally safe care. Communities in northern Canada have large populations of Aboriginal people and other marginalized groups. Our goal was to identify the elements of appropriate predeparture curricula for these medical trainees. Methods: We conducted our study in Kenora, Ontario. With the help of a core collaborative group and the support of the local Aboriginal Health Access Centre, we interviewed a purposive sample of community members about their interactions with trainees from southern Canada. Aboriginal and non-Aboriginal researchers with roots in northern and southern Canada brought perspectives to the inductive analysis. Results: We conducted 17 semistructured interviews between February and March 2014. Participants felt that southern trainees were inadequately educated in northern politics, society and history. They identified 2 more themes: determinants of health affecting the local Aboriginal population, and provider and patient factors affecting delivery of culturally competent care. Participants also shared ideas on how best to implement this content into curricula. Interpretation: Providing culturally competent care to northern communities is a complex process requiring education. Using a collaborative method, we were able to delineate the experiences of members of a northern community and identify knowledge gaps of southern trainees travelling there. Our results provide a foundation for the content and structure of formal predeparture curricula to enable such trainees to provide culturally safe care. PMID:27398374

Segmentation of pelvic structures for planning CT using a geometrical shape model tuned by a multi-scale edge detector

PubMed Central

Martínez, Fabio; Romero, Eduardo; Dréan, Gaël; Simon, Antoine; Haigron, Pascal; De Crevoisier, Renaud; Acosta, Oscar

2014-01-01

Accurate segmentation of the prostate and organs at risk in computed tomography (CT) images is a crucial step for radiotherapy (RT) planning. Manual segmentation, as performed nowadays, is a time consuming process and prone to errors due to the a high intra- and inter-expert variability. This paper introduces a new automatic method for prostate, rectum and bladder segmentation in planning CT using a geometrical shape model under a Bayesian framework. A set of prior organ shapes are first built by applying Principal Component Analysis (PCA) to a population of manually delineated CT images. Then, for a given individual, the most similar shape is obtained by mapping a set of multi-scale edge observations to the space of organs with a customized likelihood function. Finally, the selected shape is locally deformed to adjust the edges of each organ. Experiments were performed with real data from a population of 116 patients treated for prostate cancer. The data set was split in training and test groups, with 30 and 86 patients, respectively. Results show that the method produces competitive segmentations w.r.t standard methods (Averaged Dice = 0.91 for prostate, 0.94 for bladder, 0.89 for Rectum) and outperforms the majority-vote multi-atlas approaches (using rigid registration, free-form deformation (FFD) and the demons algorithm) PMID:24594798

Homo sapiens-Specific Binding Site Variants within Brain Exclusive Enhancers Are Subject to Accelerated Divergence across Human Population.

PubMed

Zehra, Rabail; Abbasi, Amir Ali

2018-03-01

Empirical assessments of human accelerated noncoding DNA frgaments have delineated presence of many cis-regulatory elements. Enhancers make up an important category of such accelerated cis-regulatory elements that efficiently control the spatiotemporal expression of many developmental genes. Establishing plausible reasons for accelerated enhancer sequence divergence in Homo sapiens has been termed significant in various previously published studies. This acceleration by including closely related primates and archaic human data has the potential to open up evolutionary avenues for deducing present-day brain structure. This study relied on empirically confirmed brain exclusive enhancers to avoid any misjudgments about their regulatory status and categorized among them a subset of enhancers with an exceptionally accelerated rate of lineage specific divergence in humans. In this assorted set, 13 distinct transcription factor binding sites were located that possessed unique existence in humans. Three of 13 such sites belonging to transcription factors SOX2, RUNX1/3, and FOS/JUND possessed single nucleotide variants that made them unique to H. sapiens upon comparisons with Neandertal and Denisovan orthologous sequences. These variants modifying the binding sites in modern human lineage were further substantiated as single nucleotide polymorphisms via exploiting 1000 Genomes Project Phase3 data. Long range haplotype based tests laid out evidence of positive selection to be governing in African population on two of the modern human motif modifying alleles with strongest results for SOX2 binding site. In sum, our study acknowledges acceleration in noncoding regulatory landscape of the genome and highlights functional parts within it to have undergone accelerated divergence in present-day human population.

Growth of Regional Acute Stroke Systems of Care in the United States in the First Decade of the 21st Century

PubMed Central

Song, Sarah; Saver, Jeffrey

2012-01-01

Background and Purpose States and counties in the US began implementing regional systems of acute stroke care in the first decade of the 21st century, whereby emergency medical services (EMS) systems preferentially route acute stroke patients directly to primary stroke centers (PSCs). The pace, geographic range, and population reach of regional stroke system implementation has not been previously delineated. Methods Review of legislative archives, internet and media reports, consultation with American Heart Association/American Stroke Association and Centers for Disease Control staff, and phone interviews with state public health and emergency medical service officials from each of the fifty states. Results The first counties to adopt regional regulations supporting routing of acute stroke patients to PSCs were in Alabama and Texas in 2000; the first states were Florida and Massachusetts in 2004. By 2010, 16 states had state-level legislation or regulations to enable EMS routing to PSCs, as did counties in 3 additional states. The US population covered by routing protocols increased substantially in the latter half of the decade, from 1.5% in 2000, to 53% of the U.S. population by the end of 2010. Conclusions The first decade of the 21st century witnessed a remarkable structural transformation in acute stroke care - by the end of 2010, over half of all Americans were living in states/counties with EMS routing protocols supporting the direct transport of acute stroke patients to primary stroke centers. Additional efforts are needed to extend regional stroke systems of care to the rest of the US. PMID:22669404

Whole Genome Sequence Typing to Investigate the Apophysomyces Outbreak following a Tornado in Joplin, Missouri, 2011

PubMed Central

Etienne, Kizee A.; Gillece, John; Hilsabeck, Remy; Schupp, Jim M.; Colman, Rebecca; Lockhart, Shawn R.; Gade, Lalitha; Thompson, Elizabeth H.; Sutton, Deanna A.; Neblett-Fanfair, Robyn; Park, Benjamin J.; Turabelidze, George; Keim, Paul; Brandt, Mary E.; Deak, Eszter; Engelthaler, David M.

2012-01-01

Case reports of Apophysomyces spp. in immunocompetent hosts have been a result of traumatic deep implantation of Apophysomyces spp. spore-contaminated soil or debris. On May 22, 2011 a tornado occurred in Joplin, MO, leaving 13 tornado victims with Apophysomyces trapeziformis infections as a result of lacerations from airborne material. We used whole genome sequence typing (WGST) for high-resolution phylogenetic SNP analysis of 17 outbreak Apophysomyces isolates and five additional temporally and spatially diverse Apophysomyces control isolates (three A. trapeziformis and two A. variabilis isolates). Whole genome SNP phylogenetic analysis revealed three clusters of genotypically related or identical A. trapeziformis isolates and multiple distinct isolates among the Joplin group; this indicated multiple genotypes from a single or multiple sources. Though no linkage between genotype and location of exposure was observed, WGST analysis determined that the Joplin isolates were more closely related to each other than to the control isolates, suggesting local population structure. Additionally, species delineation based on WGST demonstrated the need to reassess currently accepted taxonomic classifications of phylogenetic species within the genus Apophysomyces. PMID:23209631

Whole genome sequence typing to investigate the Apophysomyces outbreak following a tornado in Joplin, Missouri, 2011.

PubMed

Etienne, Kizee A; Gillece, John; Hilsabeck, Remy; Schupp, Jim M; Colman, Rebecca; Lockhart, Shawn R; Gade, Lalitha; Thompson, Elizabeth H; Sutton, Deanna A; Neblett-Fanfair, Robyn; Park, Benjamin J; Turabelidze, George; Keim, Paul; Brandt, Mary E; Deak, Eszter; Engelthaler, David M

2012-01-01

Case reports of Apophysomyces spp. in immunocompetent hosts have been a result of traumatic deep implantation of Apophysomyces spp. spore-contaminated soil or debris. On May 22, 2011 a tornado occurred in Joplin, MO, leaving 13 tornado victims with Apophysomyces trapeziformis infections as a result of lacerations from airborne material. We used whole genome sequence typing (WGST) for high-resolution phylogenetic SNP analysis of 17 outbreak Apophysomyces isolates and five additional temporally and spatially diverse Apophysomyces control isolates (three A. trapeziformis and two A. variabilis isolates). Whole genome SNP phylogenetic analysis revealed three clusters of genotypically related or identical A. trapeziformis isolates and multiple distinct isolates among the Joplin group; this indicated multiple genotypes from a single or multiple sources. Though no linkage between genotype and location of exposure was observed, WGST analysis determined that the Joplin isolates were more closely related to each other than to the control isolates, suggesting local population structure. Additionally, species delineation based on WGST demonstrated the need to reassess currently accepted taxonomic classifications of phylogenetic species within the genus Apophysomyces.

Current Concepts of Neurodegenerative Mechanisms in Alzheimer's Disease.

PubMed

Magalingam, Kasthuri Bai; Radhakrishnan, Ammu; Ping, Ng Shee; Haleagrahara, Nagaraja

2018-01-01

Neurodegenerative diseases are hereditary or sporadic conditions that result in the progressive loss of the structure and function of neurons as well as neuronal death. Although a range of diseases lie under this umbrella term, Alzheimer's disease (AD) and Parkinson's disease (PD) are the most common neurodegenerative diseases that affect a large population around the globe. Alzheimer's disease is characterized by the abnormal accumulation of extracellular amyloid- β plaques and intraneuronal neurofibrillary tangles in brain regions and manifests as a type of dementia in aged individuals that results in memory loss, multiple cognitive abnormalities, and intellectual disabilities that interfere with quality of life. Since the discovery of AD, a wealth of new information has emerged that delineates the causes, mechanisms of disease, and potential therapeutic agents, but an effective remedy to cure the diseases has not been identified yet. This could be because of the complexity of the disease process, as it involves various contributing factors that include environmental factors and genetic predispositions. This review summarizes the current understanding on neurodegenerative mechanisms that lead to the emergence of the pathology of AD.

Biological species in the viral world.

PubMed

Bobay, Louis-Marie; Ochman, Howard

2018-06-05

Due to their dependence on cellular organisms for metabolism and replication, viruses are typically named and assigned to species according to their genome structure and the original host that they infect. But because viruses often infect multiple hosts and the numbers of distinct lineages within a host can be vast, their delineation into species is often dictated by arbitrary sequence thresholds, which are highly inconsistent across lineages. Here we apply an approach to determine the boundaries of viral species based on the detection of gene flow within populations, thereby defining viral species according to the biological species concept (BSC). Despite the potential for gene transfer between highly divergent genomes, viruses, like the cellular organisms they infect, assort into reproductively isolated groups and can be organized into biological species. This approach revealed that BSC-defined viral species are often congruent with the taxonomic partitioning based on shared gene contents and host tropism, and that bacteriophages can similarly be classified in biological species. These results open the possibility to use a single, universal definition of species that is applicable across cellular and acellular lifeforms.

Temperament: a consideration of concepts and methods.

PubMed

Stevenson, J; Graham, P

1982-01-01

This paper discusses conceptual issues and begins with a consideration of definitions of temperament. It is suggested that the purposes for which temperament has been studied have, to some degree, dictated methods used and inferences drawn. Those psychopathologists interested in the relationship between temperament and psychiatric disorder, or emotional and behavioural disturbance, have tended to use different methods from those more concerned with delineating the structure of personality. Some issues and problems are common to both approaches, e.g. the definition of behaviour reflecting temperament in terms of style rather than content. Other issues, such as the difficulty involved in drawing a clear distinction between temperamental attributes and mental disorders, are restricted to one approach. The relative contribution of gentic and environmental effects is of interest to both psychopathologists (who wish to examine this issue in relation to the development of the individual), and to psychologists (who are usually more concerned with populations or aggregate effects). The application of biometric genetic models might clarify this issue, and various suggestions are made regarding steps that need to be taken if such models are to be successfully applied.

Delineating risk zones and evaluation of shelter centres for flood disaster management along the Pahang River Basin, Malaysia

PubMed Central

Isahak, Anizan; Siwar, Chamhuri; Ismail, Shaharuddin M.; Hanafi, Zulkifli; Zainuddin, Mohd S.

2018-01-01

Shelter centres are important locations to safeguard people from helpless situations and are an integral part of disaster risk reduction (DRR), particularly for flood DRR. The establishment of shelter centres, and their design based on scientific assessment, is crucial. Yet, they are very much related to the geographic location, socio-economic conditions and the livelihoods of the affected communities. However, many parts of the developing world are still lagging behind in ensuring such scientific design. Considering the flood disaster in 2014 that affected the residents living along the Pahang River Basin, in this study we delineate the communities at risk and evaluate the existing shelter centres to determine how they reduce people’s vulnerability to the risks associated with rural and urban landscapes. We used spatial analysis tools to delineate risk zones and to evaluate existing evacuation systems. A flood disaster risk map was produced to determine which communities are living with risks. Subsequently, the distribution of shelter centres examined whether they are able to support people living at the flood risk zones. These centres were also evaluated using a set of international guidelines for effective disaster shelters. This reveals that the number of shelter centres is not adequate. The designation and designing of shelter centres are not being done scientifically. The maps produced here have a lot of potential to support disaster management decisions, in particular site selection and the prioritisation of centres. The study concludes with a set of guidelines and recommendations for structural and non-structural measures, such as alternative livelihoods and the potential of ecotourism, which may improve the resilience among flood-affected communities; and the decision-making process for the overall flood DRR initiatives.

Oversight of human participants research: identifying problems to evaluate reform proposals.

PubMed

Emanuel, Ezekiel J; Wood, Anne; Fleischman, Alan; Bowen, Angela; Getz, Kenneth A; Grady, Christine; Levine, Carol; Hammerschmidt, Dale E; Faden, Ruth; Eckenwiler, Lisa; Muse, Carianne Tucker; Sugarman, Jeremy

2004-08-17

The oversight of research involving human participants is widely believed to be inadequate. The U.S. Congress, national commissions, the Department of Health and Human Services, the Institute of Medicine, numerous professional societies, and others are proposing remedies based on the assumption that the main problems are researchers' conflict of interest, lack of institutional review board (IRB) resources, and the volume and complexity of clinical research. Developing appropriate reform proposals requires carefully delineating the problems of the current system to know what reforms are needed. To stimulate a more informed and meaningful debate, we delineate 15 current problems into 3 broad categories. First, structural problems encompass 8 specific problems related to the way the research oversight system is organized. Second, procedural problems constitute 5 specific problems related to the operations of IRB review. Finally, performance assessment problems include 2 problems related to absence of systematic assessment of the outcomes of the oversight system. We critically assess proposed reforms, such as accreditation and central IRBs, according to how well they address these 15 problems. None of the reforms addresses all 15 problems. Indeed, most focus on the procedural problems, failing to address either the structure or the performance assessment problems. Finally, on the basis of the delineation of problems, we outline components of a more effective reform proposal, including bringing all research under federal oversight, a permanent advisory committee to address recurrent ethical issues in clinical research, mandatory single-time review for multicenter research protocols, additional financial support for IRB functions, and a standardized system for collecting and disseminating data on both adverse events and the performance assessment of IRBs.

Integrated geophysical investigations for the delineation of source and subsurface structure associated with hydro-uranium anomaly: A case study from South Purulia Shear Zone (SPSZ), India

NASA Astrophysics Data System (ADS)

Sharma, S. P.; Biswas, A.

2012-12-01

South Purulia Shear Zone (SPSZ) is an important region for prospecting of uranium mineralization. Geological studies and hydro-uranium anomaly suggest the presence of Uranium deposit around Raghunathpur village which lies about 8 km north of SPSZ. However, detailed geophysical investigations have not been carried out in this region for investigation of uranium mineralization. Since surface signature of uranium mineralization is not depicted near the location, a deeper subsurface source is expected for hydro uranium anomaly. To delineate the subsurface structure and to investigate the origin of hydro-uranium anomaly present in the area, Vertical Electrical Sounding (VES) using Schlumberger array and Gradient Resistivity Profiling (GRP) were performed at different locations along a profile perpendicular to the South Purulia Shear Zone. Apparent resistivity computed from the measured sounding data at various locations shows a continuously increasing trend. As a result, conventional apparent resistivity data is not able to detect the possible source of hydro uranium anomaly. An innovative approach is applied which depicts the apparent conductivity in the subsurface revealed a possible connection from SPSZ to Raghunathpur. On the other hand resistivity profiling data suggests a low resistive zone which is also characterized by low Self-Potential (SP) anomaly zone. Since SPSZ is characterized by the source of uranium mineralization; hydro-uranium anomaly at Raghunathpur is connected with the SPSZ. The conducting zone has been delineated from SPSZ to Raghunathpur at deeper depths which could be uranium bearing. Since the location is also characterized by a low gravity and high magnetic anomaly zone, this conducting zone is likely to be mineralized zone. Keywords: Apparent resistivity; apparent conductivity; Self Potential; Uranium mineralization; shear zone; hydro-uranium anomaly.

Delineation of Spatial Variability in the Temperature-Mortality Relationship on Extremely Hot Days in Greater Vancouver, Canada.

PubMed

Ho, Hung Chak; Knudby, Anders; Walker, Blake Byron; Henderson, Sarah B

2017-01-01

Climate change has increased the frequency and intensity of extremely hot weather. The health risks associated with extemely hot weather are not uniform across affected areas owing to variability in heat exposure and social vulnerability, but these differences are challenging to map with precision. We developed a spatially and temporally stratified case-crossover approach for delineation of areas with higher and lower risks of mortality on extremely hot days and applied this approach in greater Vancouver, Canada. Records of all deaths with an extremely hot day as a case day or a control day were extracted from an administrative vital statistics database spanning the years of 1998-2014. Three heat exposure and 11 social vulnerability variables were assigned at the residential location of each decedent. Conditional logistic regression was used to estimate the odds ratio for a 1°C increase in daily mean temperature at a fixed site with an interaction term for decedents living above and below different values of the spatial variables. The heat exposure and social vulnerability variables with the strongest spatially stratified results were the apparent temperature and the labor nonparticipation rate, respectively. Areas at higher risk had values ≥ 34.4°C for the maximum apparent temperature and ≥ 60% of the population neither employed nor looking for work. These variables were combined in a composite index to quantify their interaction and to enhance visualization of high-risk areas. Our methods provide a data-driven framework for spatial delineation of the temperature--mortality relationship by heat exposure and social vulnerability. The results can be used to map and target the most vulnerable areas for public health intervention. Citation: Ho HC, Knudby A, Walker BB, Henderson SB. 2017. Delineation of spatial variability in the temperature-mortality relationship on extremely hot days in greater Vancouver, Canada. Environ Health Perspect 125:66-75; http://dx.doi.org/10.1289/EHP224.

Delineation of Spatial Variability in the Temperature–Mortality Relationship on Extremely Hot Days in Greater Vancouver, Canada

PubMed Central

Ho, Hung Chak; Knudby, Anders; Walker, Blake Byron; Henderson, Sarah B.

2016-01-01

Background: Climate change has increased the frequency and intensity of extremely hot weather. The health risks associated with extemely hot weather are not uniform across affected areas owing to variability in heat exposure and social vulnerability, but these differences are challenging to map with precision. Objectives: We developed a spatially and temporally stratified case-crossover approach for delineation of areas with higher and lower risks of mortality on extremely hot days and applied this approach in greater Vancouver, Canada. Methods: Records of all deaths with an extremely hot day as a case day or a control day were extracted from an administrative vital statistics database spanning the years of 1998–2014. Three heat exposure and 11 social vulnerability variables were assigned at the residential location of each decedent. Conditional logistic regression was used to estimate the odds ratio for a 1°C increase in daily mean temperature at a fixed site with an interaction term for decedents living above and below different values of the spatial variables. Results: The heat exposure and social vulnerability variables with the strongest spatially stratified results were the apparent temperature and the labor nonparticipation rate, respectively. Areas at higher risk had values ≥ 34.4°C for the maximum apparent temperature and ≥ 60% of the population neither employed nor looking for work. These variables were combined in a composite index to quantify their interaction and to enhance visualization of high-risk areas. Conclusions: Our methods provide a data-driven framework for spatial delineation of the temperature-–mortality relationship by heat exposure and social vulnerability. The results can be used to map and target the most vulnerable areas for public health intervention. Citation: Ho HC, Knudby A, Walker BB, Henderson SB. 2017. Delineation of spatial variability in the temperature–mortality relationship on extremely hot days in greater Vancouver, Canada. Environ Health Perspect 125:66–75; http://dx.doi.org/10.1289/EHP224 PMID:27346526

The possible correlation between dental enamel hypoplasia and a historic natural disaster in the Roman population of Herculaneum (79 AD - central Italy).

PubMed

D'Anastasio, R; Cesana, D T; Viciano, J; Sciubba, M; Nibaruta, P; Capasso, L

2013-01-01

Dental enamel hypoplasia is usually read as a sign of a systematic growth disturbance during childhood. Following the analysis of human teeth from Herculaneum (79 AD, Central Italy), the authors focused on linear enamel hypoplasia (LEH) manifestations in order to delineate a possible correlation between their frequency and distribution and the earthquake that occurred in 62 AD, which is well documented in historical literature. The human remains from Herculaneum were buried at the same time as the Vesuvius eruption and represent an exceptional snapshot of life in the Roman Imperial Age. The Goodman and Rose method (1990) was used for attributing an "age at the moment of stress" for every skeleton in order to delineate the epidemiology of the enamel hypoplasia. When LEH frequency was analysed by age, two different age groups showed relevant patterns of hypoplasia: the first peak was evident in individuals between 14 and 20 years who were younger than 6 years at the time of the 62 AD earthquake, and a second peak was noted in adults of 30 +/- 5 years old, which suggests the presence of another stressful event that occurred 10 years before the earthquake, around 53 AD. The bimodal distribution of enamel hypoplasia could be the consequence of two different historical periods characterized by instability in the food supply, unhygienic conditions, and epidemic episodes; our data suggest that the first peak could be related to a decline in health status as an effect of the 62 AD earthquake. The relationship between recent natural disasters and variations in health status in modern populations is well documented in scientific literature. Our research represents the first attempt to correlate the status of health to an earthquake of known date in an archaeological population.

Geohydrologic assessment of fractured crystalline bedrock on the southern part of Manhattan, New York, through the use of advanced borehole geophysical methods

USGS Publications Warehouse

Stumm, F.; Chu, A.; Joesten, P.K.; Lane, J.W.

2007-01-01

Advanced borehole-geophysical methods were used to assess the geohydrology of fractured crystalline bedrock in 31 of 64 boreholes on the southern part of Manhattan Island, NY in preparation of the construction of a new water tunnel. The study area is located in a highly urbanized part of New York City. The boreholes penetrated gneiss, schist, and other crystalline bedrock that has an overall southwest-to northwest-dipping foliation. Most of the fractures intersected are nearly horizontal or have moderate- to high-angle northwest or eastward dip azimuths. Heat-pulse flowmeter logs obtained under nonpumping (ambient) and pumping conditions, together with other geophysical logs, delineated transmissive fracture zones in each borehole. Water-level and flowmeter data suggest the fractured-rock ground-water-flow system is interconnected. The 60 MHz directional borehole-radar logs delineated the location and orientation of several radar reflectors that did not intersect the projection of the borehole. A total of 53 faults intersected by the boreholes have mean orientation populations of N12??W, 66??W and N11??W, 70??E. A total of 77 transmissive fractures delineated using the heat-pulse flowmeter have mean orientations of N11??E, 14??SE (majority) and N23??E, 57??NW (minority). The transmissivity of the bedrock boreholes ranged from 0.7 to 870 feet squared (ft2) per day (0.07 to 81 metres squared (m2) per day). ?? 2007 Nanjing Institute of Geophysical Prospecting.

Improving depiction of temporal bone anatomy with low-radiation dose CT by an integrated circuit detector in pediatric patients: a preliminary study.

PubMed

He, Jingzhen; Zu, Yuliang; Wang, Qing; Ma, Xiangxing

2014-12-01

The purpose of this study was to determine the performance of low-dose computed tomography (CT) scanning with integrated circuit (IC) detector in defining fine structures of temporal bone in children by comparing with the conventional detector. The study was performed with the approval of our institutional review board and the patients' anonymity was maintained. A total of 86 children<3 years of age underwent imaging of temporal bone with low-dose CT (80 kV/150 mAs) equipped with either IC detector or conventional discrete circuit (DC) detector. The image noise was measured for quantitative analysis. Thirty-five structures of temporal bone were further assessed and rated by 2 radiologists for qualitative analysis. κ Statistics were performed to determine the agreement reached between the 2 radiologists on each image. Mann-Whitney U test was used to determine the difference in image quality between the 2 detector systems. Objective analysis showed that the image noise was significantly lower (P<0.001) with the IC detector than with the DC detector. The κ values for qualitative assessment of the 35 fine anatomical structures revealed high interobserver agreement. The delineation for 30 of the 35 landmarks (86%) with the IC detector was superior to that with the conventional DC detector (P<0.05) although there were no differences in the delineation of the remaining 5 structures (P>0.05). The low-dose CT images acquired with the IC detector provide better depiction of fine osseous structures of temporal bone than that with the conventional DC detector.

Magnetic Transfer Contrast Accurately Localizes Substantia Nigra Confirmed by Histology

PubMed Central

Bolding, Mark S.; Reid, Meredith A.; Avsar, Kathy B.; Roberts, Rosalinda C.; Gamlin, Paul D.; Gawne, Timothy J.; White, David M.; den Hollander, Jan A.; Lahti, Adrienne C.

2012-01-01

Background Magnetic Resonance Imaging (MRI) has multiple contrast mechanisms. Like various staining techniques in histology, each contrast type reveals different information about the structure of the brain. However, it is not always clear how structures visible in MRI correspond to structures previously identified by histology. The purpose of this study was to determine if magnetic transfer contrast (MTC) or T2 contrast MRI was better at delineating the substantia nigra. Methods MRI scans were acquired in-vivo from two non-human primates (NHPs). The NHPs were subsequently euthanized, perfused, and their brains sectioned for histological analyses. Each slice was photographed prior to sectioning. Each brain was sectioned into approximately 500, 40-micron sections, encompassing most of the cortex, midbrain, and dorsal parts of the hindbrain. Levels corresponding to anatomical MRI images were selected. From these, adjacent sections were stained using Kluver Barrera (myelin and cell bodies) or tyrosine hydroxylase (TH) (dopaminergic neurons) immunohistochemistry. The resulting images were coregistered to the block-face images using a moving least squares algorithm with similarity transformations. MR images were similarly coregistered to the block-face images, allowing the structures in the MRI to be identified with structures in the histological images. Results We found that hyperintense (light) areas in MTC images were coextensive with the SN as delineated histologically. The hypointense (dark) areas in T2-weighted images were not coextensive with the SN, but extended partially into the SN and partially into the cerebral peduncles. Conclusions MTC is a more accurate contrast mechanism than T2-weighting for localizing the SN in vivo. PMID:22981657

Gaining insight of fetal brain development with diffusion MRI and histology.

PubMed

Huang, Hao; Vasung, Lana

2014-02-01

Human brain is extraordinarily complex and yet its origin is a simple tubular structure. Its development during the fetal period is characterized by a series of accurately organized events which underlie the mechanisms of dramatic structural changes during fetal development. Revealing detailed anatomy at different stages of human fetal brain development provides insight on understanding not only this highly ordered process, but also the neurobiological foundations of cognitive brain disorders such as mental retardation, autism, schizophrenia, bipolar and language impairment. Diffusion tensor imaging (DTI) and histology are complementary tools which are capable of delineating the fetal brain structures at both macroscopic and microscopic levels. In this review, the structural development of the fetal brains has been characterized with DTI and histology. Major components of the fetal brain, including cortical plate, fetal white matter and cerebral wall layer between the ventricle and subplate, have been delineated with DTI and histology. Anisotropic metrics derived from DTI were used to quantify the microstructural changes during the dynamic process of human fetal cortical development and prenatal development of other animal models. Fetal white matter pathways have been traced with DTI-based tractography to reveal growth patterns of individual white matter tracts and corticocortical connectivity. These detailed anatomical accounts of the structural changes during fetal period may provide the clues of detecting developmental and cognitive brain disorders at their early stages. The anatomical information from DTI and histology may also provide reference standards for diagnostic radiology of premature newborns. Copyright © 2013 ISDN. Published by Elsevier Ltd. All rights reserved.

Local site preference rationalizes disentangling by DNA topoisomerases

NASA Astrophysics Data System (ADS)

Liu, Zhirong; Zechiedrich, Lynn; Chan, Hue Sun

2010-03-01

To rationalize the disentangling action of type II topoisomerases, an improved wormlike DNA model was used to delineate the degree of unknotting and decatenating achievable by selective segment passage at specific juxtaposition geometries and to determine how these activities were affected by DNA circle size and solution ionic strength. We found that segment passage at hooked geometries can reduce knot populations as dramatically as seen in experiments. Selective segment passage also provided theoretical underpinning for an intriguing empirical scaling relation between unknotting and decatenating potentials.

How to use: the neonatal neurological examination.

PubMed

Wusthoff, Courtney J

2013-08-01

The neurological exam can be a challenging part of a newborn's full evaluation. At the same time, the neonatal neurological exam is a useful tool in identifying babies needing closer evaluation for potential problems. The Dubowitz assessment is a standardised approach to the neonatal neurological exam designed for use by paediatricians in routine practice. Evidence has validated this technique and delineated its utility as a screening exam in various populations. This paper reviews clinical application of the Dubowitz assessment of the newborn.

Using aquatic invertebrates to delineate seasonal and temporary wetlands in the Prairie Pothole Region of North America

USGS Publications Warehouse

Euliss, Ned H.; Mushet, David M.; Johnson, Douglas H.

2002-01-01

Tillage can destroy or greatly disturb indicators of hydric soils and hydrophytic vegetation, making delineation of tilled wetlands difficult. The remains of aquatic invertebrates (e.g., shells, drought-resistant eggs, and trichopteran cases) are easily identifiable and persist in wetland substrates even when wetlands are dry. Additionally, these remains are not easily destroyed by mechanical tillage. To test the feasibility of using invertebrate remains to delineate wetlands, we used two methods to identify the wetland edge of ten seasonal and ten temporary wetlands, evenly divided between grassland and cropland landscapes. First, we identified the wetland edge using hydric soil and vegetation indicators along six evenly spaced transects in each wetland (our “standard” delineation). We then identified the wetland edge along the same transects using aquatic invertebrate remains as our indicator. In grassland landscapes, delineations of the wetland edge made using invertebrate remains were consistently at the same location or closer to the wetland center as the standard delineations for both seasonal and temporary wetlands. In cropland landscapes, however, many of our invertebrate delineations of seasonal and temporary wetlands were on the upland side of our standard delineations. We attribute the differences to movement of remains during tillage, increased maximum pool levels in cropland wetlands, and disturbance of hydric soils and plants. We found that the elevations of the wetland edge indicated by invertebrate remains were more consistent within a wetland than elevations determined by standard delineations. Aquatic invertebrate remains can be useful in delineating wetlands when other indicators have been destroyed or severely disturbed by tillage.

Assessing the Agreement Between Eo-Based Semi-Automated Landslide Maps with Fuzzy Manual Landslide Delineation

NASA Astrophysics Data System (ADS)

Albrecht, F.; Hölbling, D.; Friedl, B.

2017-09-01

Landslide mapping benefits from the ever increasing availability of Earth Observation (EO) data resulting from programmes like the Copernicus Sentinel missions and improved infrastructure for data access. However, there arises the need for improved automated landslide information extraction processes from EO data while the dominant method is still manual delineation. Object-based image analysis (OBIA) provides the means for the fast and efficient extraction of landslide information. To prove its quality, automated results are often compared to manually delineated landslide maps. Although there is awareness of the uncertainties inherent in manual delineations, there is a lack of understanding how they affect the levels of agreement in a direct comparison of OBIA-derived landslide maps and manually derived landslide maps. In order to provide an improved reference, we present a fuzzy approach for the manual delineation of landslides on optical satellite images, thereby making the inherent uncertainties of the delineation explicit. The fuzzy manual delineation and the OBIA classification are compared by accuracy metrics accepted in the remote sensing community. We have tested this approach for high resolution (HR) satellite images of three large landslides in Austria and Italy. We were able to show that the deviation of the OBIA result from the manual delineation can mainly be attributed to the uncertainty inherent in the manual delineation process, a relevant issue for the design of validation processes for OBIA-derived landslide maps.

Spatial and thematic assessment of object-based forest stand delineation using an OFA-matrix

NASA Astrophysics Data System (ADS)

Hernando, A.; Tiede, D.; Albrecht, F.; Lang, S.

2012-10-01

The delineation and classification of forest stands is a crucial aspect of forest management. Object-based image analysis (OBIA) can be used to produce detailed maps of forest stands from either orthophotos or very high resolution satellite imagery. However, measures are then required for evaluating and quantifying both the spatial and thematic accuracy of the OBIA output. In this paper we present an approach for delineating forest stands and a new Object Fate Analysis (OFA) matrix for accuracy assessment. A two-level object-based orthophoto analysis was first carried out to delineate stands on the Dehesa Boyal public land in central Spain (Avila Province). Two structural features were first created for use in class modelling, enabling good differentiation between stands: a relational tree cover cluster feature, and an arithmetic ratio shadow/tree feature. We then extended the OFA comparison approach with an OFA-matrix to enable concurrent validation of thematic and spatial accuracies. Its diagonal shows the proportion of spatial and thematic coincidence between a reference data and the corresponding classification. New parameters for Spatial Thematic Loyalty (STL), Spatial Thematic Loyalty Overall (STLOVERALL) and Maximal Interfering Object (MIO) are introduced to summarise the OFA-matrix accuracy assessment. A stands map generated by OBIA (classification data) was compared with a map of the same area produced from photo interpretation and field data (reference data). In our example the OFA-matrix results indicate good spatial and thematic accuracies (>65%) for all stand classes except for the shrub stands (31.8%), and a good STLOVERALL (69.8%). The OFA-matrix has therefore been shown to be a valid tool for OBIA accuracy assessment.

Modeling individual trees in an urban environment using dense discrete return LIDAR

NASA Astrophysics Data System (ADS)

Bandyopadhyay, Madhurima; van Aardt, Jan A. N.; van Leeuwen, Martin

2015-05-01

The urban forest is becoming increasingly important in the contexts of urban green space, carbon sequestration and offsets, and socio-economic impacts. This has led to a recent increase in attention being paid to urban environmental management. Tree biomass, specifically, is a vital indicator of carbon storage and has a direct impact on urban forest health and carbon sequestration. As an alternative to expensive and time-consuming field surveys, remote sensing has been used extensively in measuring dynamics of vegetation and estimating biomass. Light detection and ranging (LiDAR) has proven especially useful to characterize the three dimensional (3D) structure of forests. In urban contexts however, information is frequently required at the individual tree level, necessitating the proper delineation of tree crowns. Yet, crown delineation is challenging for urban trees where a wide range of stress factors and cultural influences affect growth. In this paper high resolution LiDAR data were used to infer biomass based on individual tree attributes. A multi-tiered delineation algorithm was designed to extract individual tree-crowns. At first, dominant tree segments were obtained by applying watershed segmentation on the crown height model (CHM). Next, prominent tree top positions within each segment were identified via a regional maximum transformation and the crown boundary was estimated for each of the tree tops. Finally, undetected trees were identified using a best-fitting circle approach. After tree delineation, individual tree attributes were used to estimate tree biomass and the results were validated with associated field mensuration data. Results indicate that the overall tree detection accuracy is nearly 80%, and the estimated biomass model has an adjusted-R2 of 0.5.

Horizontal gravity gradient - An aid to the definition of crustal structure in North America

NASA Technical Reports Server (NTRS)

Sharpton, V. L.; Grieve, R. A. F.; Thomas, M. D.; Halpenny, J. F.

1987-01-01

A map of the magnitude of the horizontal Bouguer gravity gradient over the North American continent is used to delineate lateral discontinuities in upper crustal density and/or thickness associated with such processes as suturing and rifting. The usefulness of gradient trends in mapping major structural boundaries, which are sometimes poorly exposed or completely buried, is demonstrated by examples such as the buried southward extension of the Grenville Front and buried boundaries of the Superior Province. Gradient trends also draw attention to poorly known structures, which may have major tectonic significance, and to a continent-wide structural fabric, which may provide a record of the tectonic growth of the North American continent.

Preliminary investigation of structural controls of ground-water movement in Pipe Spring National Monument, Arizona

USGS Publications Warehouse

Truini, Margot; Fleming, John B.; Pierce, Herb A.

2004-01-01

Pipe Spring National Monument, near the border of Arizona and Utah, includes several low-discharge springs that are the primary natural features of the monument. The National Park Service is concerned about the declines in spring discharge. Seismic-refraction and frequency-domain electromagnetic-induction methods were employed in an attempt to better understand the relation between spring discharge and geologic structure. The particular method used for the seismic-refraction surveys was unable to resolve structural features in the monument. Electromagnetic surveys delineated differences in apparent conductivity of the shallow subsurface deposits. The differences are attributable to differences in saturation, lithology, and structure of these deposits.

Regional Supplement to the Corps of Engineers Wetland Delineation Manual: Alaska Region (Version 2.0)

DTIC Science & Technology

2007-09-01

07-24 102 Viereck, L. A., K. Van Cleve, and C. T. Dyrness. 1986. Forest ecosystem distribution in the Taiga environment, Chapter 3. In Forest...ecosystems in the Alaska Taiga : A synthesis of structure and function. ed. K. Van Cleve, F. S. Chapin III, P. W. Flanagan, L. A. Viereck, and C. T

Using structure locations as a basis for mapping the wildland urban interface

Treesearch

Avi Bar-Massada; Susan I. Stewart; Roger B. Hammer; Miranda H. Mockrin; Volker C. Radeloff

2013-01-01

The wildland urban interface (WUI) delineates the areas where wildland fire hazard most directly impacts human communities and threatens lives and property, and where houses exert the strongest influence on the natural environment. Housing data are a major problem for WUI mapping. When housing data are zonal, the concept of a WUI neighborhood can be captured easily in...

Development of deployable structures for large space platform systems, volume 1

NASA Technical Reports Server (NTRS)

1982-01-01

Generic deployable spacecraft configurations and deployable platform systems concepts were identified. Sizing, building block concepts, orbiter packaging, thermal analysis, cost analysis, and mass properties analysis as related to platform systems integration are considered. Technology needs are examined and the major criteria used in concept selection are delineated. Requirements for deployable habitat modules, tunnels, and OTV hangars are considered.

Automatic thoracic anatomy segmentation on CT images using hierarchical fuzzy models and registration

NASA Astrophysics Data System (ADS)

Sun, Kaioqiong; Udupa, Jayaram K.; Odhner, Dewey; Tong, Yubing; Torigian, Drew A.

2014-03-01

This paper proposes a thoracic anatomy segmentation method based on hierarchical recognition and delineation guided by a built fuzzy model. Labeled binary samples for each organ are registered and aligned into a 3D fuzzy set representing the fuzzy shape model for the organ. The gray intensity distributions of the corresponding regions of the organ in the original image are recorded in the model. The hierarchical relation and mean location relation between different organs are also captured in the model. Following the hierarchical structure and location relation, the fuzzy shape model of different organs is registered to the given target image to achieve object recognition. A fuzzy connected delineation method is then used to obtain the final segmentation result of organs with seed points provided by recognition. The hierarchical structure and location relation integrated in the model provide the initial parameters for registration and make the recognition efficient and robust. The 3D fuzzy model combined with hierarchical affine registration ensures that accurate recognition can be obtained for both non-sparse and sparse organs. The results on real images are presented and shown to be better than a recently reported fuzzy model-based anatomy recognition strategy.

Satellite geological and geophysical remote sensing of Iceland

NASA Technical Reports Server (NTRS)

Williams, R. S., Jr. (Principal Investigator)

1974-01-01

The author has identified the following significant results. ERTS-1 imagery provides sufficient resolution to discern two effects of geothermal activity at the Namafjall geothermal area: snowmelt anomalies and delineation of altered ground. The fallout pattern of tephra from Hekla's 1970 volcanic eruption can be mapped where sufficient depth of deposition destroyed the vegetation. Lava flows from the volcanic eruptions at Askja and Hekla can be delineated. Low sun-angle imagery of snow-covered terrain has permitted the mapping of new structural and volcanic features beneath the icecaps. Coastline changes on the islands of Surtsey and Heimaey can be mapped. Variations of sediment plumes from glacial rivers on the south coast give a qualitative indication of seasonal changes in melting rates of glaciers. ERTS-1 imagery has been shown to be especially amenable to portrayal of changing glaciological phenomena: surging glaciers, collapse features in icecaps caused by subglacial volcanic (?) and geothermal activity and resulting jokulhlaups, and variations in size of glacier-margin lakes. A fifth vegetation class has now been added: lichen-covered bedrock. The high latitude permits more precise analysis of landforms, vegetation distribution, occurrence of snow cover, glaciers, and geologic structure.

Delineating the Impact of Weightlessness on Human Physiology Using Computational Models

NASA Technical Reports Server (NTRS)

Kassemi, Mohammad

2015-01-01

Microgravity environment has profound effects on several important human physiological systems. The impact of weightlessness is usually indirect as mediated by changes in the biological fluid flow and transport and alterations in the deformation and stress fields of the compliant tissues. In this context, Fluid-Structural and Fluid-Solid Interaction models provide a valuable tool in delineating the physical origins of the physiological changes so that systematic countermeasures can be devised to reduce their adverse effects. In this presentation, impact of gravity on three human physiological systems will be considered. The first case involves prediction of cardiac shape change and altered stress distributions in weightlessness. The second, presents a fluid-structural-interaction (FSI) analysis and assessment of the vestibular system and explores the reasons behind the unexpected microgravity caloric stimulation test results performed aboard the Skylab. The last case investigates renal stone development in microgravity and the possible impact of re-entry into partial gravity on the development and transport of nucleating, growing, and agglomerating renal calculi in the nephron. Finally, the need for model validation and verification and application of the FSI models to assess the effects of Artificial Gravity (AG) are also briefly discussed.

Biofilms in 3D porous media: Delineating the influence of the pore network geometry, flow and mass transfer on biofilm development.

PubMed

Carrel, Maxence; Morales, Verónica L; Beltran, Mario A; Derlon, Nicolas; Kaufmann, Rolf; Morgenroth, Eberhard; Holzner, Markus

2018-05-01

This study investigates the functional correspondence between porescale hydrodynamics, mass transfer, pore structure and biofilm morphology during progressive biofilm colonization of a porous medium. Hydrodynamics and the structure of both the porous medium and the biofilm are experimentally measured with 3D particle tracking velocimetry and micro X-ray Computed Tomography, respectively. The analysis focuses on data obtained in a clean porous medium after 36 h of biofilm growth. Registration of the particle tracking and X-ray data sets allows to delineate the interplay between porous medium geometry, hydrodynamic and mass transfer processes on the morphology of the developing biofilm. A local analysis revealed wide distributions of wall shear stresses and concentration boundary layer thicknesses. The spatial distribution of the biofilm patches uncovered that the wall shear stresses controlled the biofilm development. Neither external nor internal mass transfer limitations were noticeable in the considered system, consistent with the excess supply of nutrient and electron acceptors. The wall shear stress remained constant in the vicinity of the biofilm but increased substantially elsewhere. Copyright © 2018 Elsevier Ltd. All rights reserved.

MRI Post-processing in Pre-surgical Evaluation

PubMed Central

Wang, Z. Irene; Alexopoulos, Andreas V.

2016-01-01

Purpose of Review Advanced MRI post-processing techniques are increasingly used to complement visual analysis and elucidate structural epileptogenic lesions. This review summarizes recent developments in MRI post-processing in the context of epilepsy pre-surgical evaluation, with the focus on patients with unremarkable MRI by visual analysis (i.e., “nonlesional” MRI). Recent Findings Various methods of MRI post-processing have been reported to show additional clinical values in the following areas: (1) lesion detection on an individual level; (2) lesion confirmation for reducing the risk of over reading the MRI; (3) detection of sulcal/gyral morphologic changes that are particularly difficult for visual analysis; and (4) delineation of cortical abnormalities extending beyond the visible lesion. Future directions to improve performance of MRI post-processing include using higher magnetic field strength for better signal and contrast to noise ratio, adopting a multi-contrast frame work, and integration with other noninvasive modalities. Summary MRI post-processing can provide essential value to increase the yield of structural MRI and should be included as part of the presurgical evaluation of nonlesional epilepsies. MRI post-processing allows for more accurate identification/delineation of cortical abnormalities, which should then be more confidently targeted and mapped. PMID:26900745

Use of optical coherence tomography in delineating airways microstructure: comparison of OCT images to histopathological sections

NASA Astrophysics Data System (ADS)

Yang, Ying; Whiteman, Suzanne; Gey van Pittius, Daniel; He, Yonghong; Wang, Ruikang K.; Spiteri, Monica A.

2004-04-01

An ideal diagnostic system for the human airways should be able to detect and define early development of premalignant pathological lesions, to facilitate optimal curative treatment and prevent irreversible and/or invasive lung disease. There is great need for exploration of safe, repeatable imaging techniques which can run at real-time and with high spatial resolution. In this study, optical coherence tomography (OCT) was utilized to acquire cross-sectional images of upper and lower airways using fresh pig lung resections as a model system. Obtained OCT images were compared with parallel tissue characterization by conventional histological analysis. Our objective was to determine whether OCT differentiates the composite structural layers and inherent anatomical variations along different airway locations. The data show that OCT can clearly display the multilayered structure of the airways. The subtle architectural differences in three separate anatomical locations including trachea, main bronchus and tertiary bronchus were clearly delineated. Images of the appropriate anatomical profiles, with depth of up to 2 mm and 10 µm spatial resolution were obtained by our current OCT system, which was sufficient for recognition of the epithelium, subepithelial tissues and cartilage. In addition, the relative thickness of individual structural components was accurately reflected and comparable to histological sections. These data support OCT as a highly feasible, optical biopsy tool, which merits further exploration for early diagnosis of human airway epithelial pathology.

The pre-Cainozoic basement delineation by magnetotelluric methods in the western part of the Liptovská kotlina Depression (Western Carpathians, Slovakia)

NASA Astrophysics Data System (ADS)

Fendek, Marián; Grand, Tomáš; Daniel, Slavomír; Blanárová, Veronika; Kultan, Vincent; Bielik, Miroslav

2017-08-01

The geology and hydrogeology of the Liptovská Kotlina Depression were studied by means of new geophysical methods. Controlled source audio-frequency magnetotellurics enabled us to delineate the relief of the pre-Cainozoic basement in the western part of the Liptovská Kotlina Depression into two segments with different lithostratigraphic units. Our complex findings disprove the interconnection between the Bešeňová and Lúčky water bearing structures located in the study area. The results were interpreted in the form of a resistivity cross section and resistivity model. The geological interpretation of the obtained results, taking into account the other geophysical and geological constrains showed that the pre-Cainozoic basement has a tectonically disrupted, broken relief. The Bešeňová and Lúčky structures appear to be isolated by the Palaeogene sediments (sandstone, claystone) and in the deeper part also by marly carbonates and marlstones of the Jurassic age belonging to the Fatricum. It was confirmed that the structural connectivity of geothermal aquifers in the area between the Bešeňová and Lúčky-Kaľameny should not exist. The assumption of different circulation depth was also confirmed by geothermometry and existing radiocarbon analyses applied on groundwater in both areas.

Utilizing potential field data to support delineation of groundwater aquifers in the southern Red Sea coast, Saudi Arabia

NASA Astrophysics Data System (ADS)

Elawadi, Eslam; Mogren, Saad; Ibrahim, Elkhedr; Batayneh, Awni; Al-Bassam, Abdulaziz

2012-06-01

In this paper potential field data are interpreted to map the undulation of the basement surface, which represents the bottom of the water bearing zones, and to delineate the tectonic framework that controls the groundwater flow and accumulation in the southern Red Sea coastal area of Saudi Arabia. The interpretation reveals that the dominant structural trend is a NW (Red Sea) trend that resulted in a series of faulted tilted blocks. These tilted blocks are dissected by another cross-cut NE trend which shapes and forms a series of fault-bounded small basins. These basins and the bounded structural trends control and shape the flow direction of the groundwater in the study area, i.e. they act as groundwater conduits. Furthermore, the present results indicate that volcanic intrusions are present as subsurface flows, which hinder the groundwater exploration and drilling activities in most of the area; in some localities these volcanic flows crop out at the surface and cover the groundwater bearing formations. Furthermore, the gravity and magnetic data interpretation indicates the possible existence of a large structural basin occupying the southeastern side of the study area. This basin is bounded with NW and NE trending faults and is expected to be a good host for groundwater aquifers; thus it is a promising site for hydrogeological investigation.

Commentary: Leveraging discovery science to advance child and adolescent psychiatric research--a commentary on Zhao and Castellanos 2016.

PubMed

Mennes, Maarten

2016-03-01

'Big Data' and 'Population Imaging' are becoming integral parts of inspiring research aimed at delineating the biological underpinnings of psychiatric disorders. The scientific strategies currently associated with big data and population imaging are typically embedded in so-called discovery science, thereby pointing to the hypothesis-generating rather than hypothesis-testing nature of discovery science. In this issue, Yihong Zhao and F. Xavier Castellanos provide a compelling overview of strategies for discovery science aimed at progressing our understanding of neuropsychiatric disorders. In particular, they focus on efforts in genetic and neuroimaging research, which, together with extended behavioural testing, form the main pillars of psychopathology research. © 2016 Association for Child and Adolescent Mental Health.

Core data elements tracking elder sexual abuse.

PubMed

Hanrahan, Nancy P; Burgess, Ann W; Gerolamo, Angela M

2005-05-01

Sexual abuse in the older adult population is an understudied vector of violent crimes with significant physical and psychological consequences for victims and families. Research requires a theoretical framework that delineates core elements using a standardized instrument. To develop a conceptual framework and identify core data elements specific to the older adult population, clinical, administrative, and criminal experts were consulted using a nominal group method to revise an existing sexual assault instrument. The revised instrument could be used to establish a national database of elder sexual abuse. The database could become a standard reference to guide the detection, assessment, and prosecution of elder sexual abuse crimes as well as build a base from which policy makers could plan and evaluate interventions that targeted risk factors.

CA1 pyramidal cell diversity enabling parallel information processing in the hippocampus

PubMed Central

Soltesz, Ivan; Losonczy, Attila

2018-01-01

Hippocampal network operations supporting spatial navigation and declarative memory are traditionally interpreted in a framework where each hippocampal area, such as the dentate gyrus, CA3, and CA1, consists of homogeneous populations of functionally equivalent principal neurons. However, heterogeneity within hippocampal principal cell populations, in particular within pyramidal cells at the main CA1 output node, is increasingly recognized and includes developmental, molecular, anatomical, and functional differences. Here we review recent progress in the delineation of hippocampal principal cell subpopulations by focusing on radially defined subpopulations of CA1 pyramidal cells, and we consider how functional segregation of information streams, in parallel channels with nonuniform properties, could represent a general organizational principle of the hippocampus supporting diverse behaviors. PMID:29593317

Headwater Capture Evidenced by Paleo-Rivers Reconstruction and Population Genetic Structure of the Armored Catfish (Pareiorhaphis garbei) in the Serra do Mar Mountains of Southeastern Brazil

PubMed Central

Lima, Sergio M. Q.; Berbel-Filho, Waldir M.; Araújo, Thais F. P.; Lazzarotto, Henrique; Tatarenkov, Andrey; Avise, John C.

2017-01-01

Paleo-drainage connections and headwater stream-captures are two main historical processes shaping the distribution of strictly freshwater fishes. Recently, bathymetric-based methods of paleo-drainage reconstruction have opened new possibilities to investigate how these processes have shaped the genetic structure of freshwater organisms. In this context, the present study used paleo-drainage reconstructions and single-locus cluster delimitation analyses to examine genetic structure on the whole distribution of Pareiorhaphis garbei, a ‘near threatened’ armored catfish from the Fluminense freshwater ecoregion in Southeastern Brazil. Sequences of two mitochondrial genes (cytochrome b and cytochrome c oxidase subunit 1) were obtained from five sampling sites in four coastal drainages: Macaé (KAE), São João (SJO), Guapi-Macacu [sub-basins Guapiaçu (GAC) and Guapimirim (GMI)], and Santo Aleixo (SAL). Pronounced genetic structure was found, involving 10 haplotypes for cytB and 6 for coi, with no haplotypes shared between localities. Coalescent-based delineation methods as well as distance-based methods revealed genetic clusters corresponding to each sample site. Paleo-drainage reconstructions showed two putative paleo-rivers: an eastern one connecting KAE and SJO; and a western one merging in the Guanabara Bay (GAC, GMI, and SAL). A disagreement was uncovered between the inferred past riverine connections and current population genetic structure. Although KAE and SJO belong to the same paleo-river, the latter is more closely related to specimens from the Guanabara paleo-river. This discordance between paleo-drainage connections and phylogenetic structure may indicate an ancient stream-capture event in headwaters of this region. Furthermore, all analyses showed high divergence between KAE and the other lineages, suggesting at least one cryptic species in the latter, and that the nominal species should be restricted to the Macaé river basin, its type locality. In this drainage, impacts such as the invasive species and habitat loss can be especially threatening for such species with a narrow range. Our results also suggest that freshwater fishes from headwaters in the Serra do Mar mountains might have different biogeographical patterns than those from the lowlands, indicating a complex and dynamic climatic and geomorphological history. PMID:29259623

Headwater Capture Evidenced by Paleo-Rivers Reconstruction and Population Genetic Structure of the Armored Catfish (Pareiorhaphis garbei) in the Serra do Mar Mountains of Southeastern Brazil.

PubMed

Lima, Sergio M Q; Berbel-Filho, Waldir M; Araújo, Thais F P; Lazzarotto, Henrique; Tatarenkov, Andrey; Avise, John C

2017-01-01

Paleo-drainage connections and headwater stream-captures are two main historical processes shaping the distribution of strictly freshwater fishes. Recently, bathymetric-based methods of paleo-drainage reconstruction have opened new possibilities to investigate how these processes have shaped the genetic structure of freshwater organisms. In this context, the present study used paleo-drainage reconstructions and single-locus cluster delimitation analyses to examine genetic structure on the whole distribution of Pareiorhaphis garbei , a 'near threatened' armored catfish from the Fluminense freshwater ecoregion in Southeastern Brazil. Sequences of two mitochondrial genes (cytochrome b and cytochrome c oxidase subunit 1) were obtained from five sampling sites in four coastal drainages: Macaé (KAE), São João (SJO), Guapi-Macacu [sub-basins Guapiaçu (GAC) and Guapimirim (GMI)], and Santo Aleixo (SAL). Pronounced genetic structure was found, involving 10 haplotypes for cytB and 6 for coi , with no haplotypes shared between localities. Coalescent-based delineation methods as well as distance-based methods revealed genetic clusters corresponding to each sample site. Paleo-drainage reconstructions showed two putative paleo-rivers: an eastern one connecting KAE and SJO; and a western one merging in the Guanabara Bay (GAC, GMI, and SAL). A disagreement was uncovered between the inferred past riverine connections and current population genetic structure. Although KAE and SJO belong to the same paleo-river, the latter is more closely related to specimens from the Guanabara paleo-river. This discordance between paleo-drainage connections and phylogenetic structure may indicate an ancient stream-capture event in headwaters of this region. Furthermore, all analyses showed high divergence between KAE and the other lineages, suggesting at least one cryptic species in the latter, and that the nominal species should be restricted to the Macaé river basin, its type locality. In this drainage, impacts such as the invasive species and habitat loss can be especially threatening for such species with a narrow range. Our results also suggest that freshwater fishes from headwaters in the Serra do Mar mountains might have different biogeographical patterns than those from the lowlands, indicating a complex and dynamic climatic and geomorphological history.

Extracting topographic structure from digital elevation data for geographic information-system analysis

USGS Publications Warehouse

Jenson, Susan K.; Domingue, Julia O.

1988-01-01

The first phase of analysis is a conditioning phase that generates three data sets: the original OEM with depressions filled, a data set indicating the flow direction for each cell, and a flow accumulation data set in which each cell receives a value equal to the total number of cells that drain to it. The original OEM and these three derivative data sets can then be processed in a variety of ways to optionally delineate drainage networks, overland paths, watersheds for userspecified locations, sub-watersheds for the major tributaries of a drainage network, or pour point linkages between watersheds. The computer-generated drainage lines and watershed polygons and the pour point linkage information can be transferred to vector-based geographic information systems for futher analysis. Comparisons between these computergenerated features and their manually delineated counterparts generally show close agreement, indicating that these software tools will save analyst time spent in manual interpretation and digitizing.

Detailed Hydrographic Feature Extraction from High-Resolution LiDAR Data

DOE Office of Scientific and Technical Information (OSTI.GOV)

Danny L. Anderson

Detailed hydrographic feature extraction from high-resolution light detection and ranging (LiDAR) data is investigated. Methods for quantitatively evaluating and comparing such extractions are presented, including the use of sinuosity and longitudinal root-mean-square-error (LRMSE). These metrics are then used to quantitatively compare stream networks in two studies. The first study examines the effect of raster cell size on watershed boundaries and stream networks delineated from LiDAR-derived digital elevation models (DEMs). The study confirmed that, with the greatly increased resolution of LiDAR data, smaller cell sizes generally yielded better stream network delineations, based on sinuosity and LRMSE. The second study demonstrates amore » new method of delineating a stream directly from LiDAR point clouds, without the intermediate step of deriving a DEM. Direct use of LiDAR point clouds could improve efficiency and accuracy of hydrographic feature extractions. The direct delineation method developed herein and termed “mDn”, is an extension of the D8 method that has been used for several decades with gridded raster data. The method divides the region around a starting point into sectors, using the LiDAR data points within each sector to determine an average slope, and selecting the sector with the greatest downward slope to determine the direction of flow. An mDn delineation was compared with a traditional grid-based delineation, using TauDEM, and other readily available, common stream data sets. Although, the TauDEM delineation yielded a sinuosity that more closely matches the reference, the mDn delineation yielded a sinuosity that was higher than either the TauDEM method or the existing published stream delineations. Furthermore, stream delineation using the mDn method yielded the smallest LRMSE.« less

Measuring the apparent diffusion coefficient in primary rectal tumors: is there a benefit in performing histogram analyses?

PubMed

van Heeswijk, Miriam M; Lambregts, Doenja M J; Maas, Monique; Lahaye, Max J; Ayas, Z; Slenter, Jos M G M; Beets, Geerard L; Bakers, Frans C H; Beets-Tan, Regina G H

2017-06-01

The apparent diffusion coefficient (ADC) is a potential prognostic imaging marker in rectal cancer. Typically, mean ADC values are used, derived from precise manual whole-volume tumor delineations by experts. The aim was first to explore whether non-precise circular delineation combined with histogram analysis can be a less cumbersome alternative to acquire similar ADC measurements and second to explore whether histogram analyses provide additional prognostic information. Thirty-seven patients who underwent a primary staging MRI including diffusion-weighted imaging (DWI; b0, 25, 50, 100, 500, 1000; 1.5 T) were included. Volumes-of-interest (VOIs) were drawn on b1000-DWI: (a) precise delineation, manually tracing tumor boundaries (2 expert readers), and (b) non-precise delineation, drawing circular VOIs with a wide margin around the tumor (2 non-experts). Mean ADC and histogram metrics (mean, min, max, median, SD, skewness, kurtosis, 5th-95th percentiles) were derived from the VOIs and delineation time was recorded. Measurements were compared between the two methods and correlated with prognostic outcome parameters. Median delineation time reduced from 47-165 s (precise) to 21-43 s (non-precise). The 45th percentile of the non-precise delineation showed the best correlation with the mean ADC from the precise delineation as the reference standard (ICC 0.71-0.75). None of the mean ADC or histogram parameters showed significant prognostic value; only the total tumor volume (VOI) was significantly larger in patients with positive clinical N stage and mesorectal fascia involvement. When performing non-precise tumor delineation, histogram analysis (in specific 45th ADC percentile) may be used as an alternative to obtain similar ADC values as with precise whole tumor delineation. Histogram analyses are not beneficial to obtain additional prognostic information.

Sanitary protection zoning based on time-dependent vulnerability assessment model - case examples at two different type of aquifers

NASA Astrophysics Data System (ADS)

Živanović, Vladimir; Jemcov, Igor; Dragišić, Veselin; Atanacković, Nebojša

2017-04-01

Delineation of sanitary protection zones of groundwater source is a comprehensive and multidisciplinary task. Uniform methodology for protection zoning for various type of aquifers is not established. Currently applied methods mostly rely on horizontal groundwater travel time toward the tapping structure. On the other hand, groundwater vulnerability assessment methods evaluate the protective function of unsaturated zone as an important part of groundwater source protection. In some particular cases surface flow might also be important, because of rapid transfer of contaminants toward the zones with intense infiltration. For delineation of sanitary protection zones three major components should be analysed: vertical travel time through unsaturated zone, horizontal travel time through saturated zone and surface water travel time toward intense infiltration zones. Integrating the aforementioned components into one time-dependent model represents a basis of presented method for delineation of groundwater source protection zones in rocks and sediments of different porosity. The proposed model comprises of travel time components of surface water, as well as groundwater (horizontal and vertical component). The results obtained using the model, represent the groundwater vulnerability as the sum of the surface and groundwater travel time and corresponds to the travel time of potential contaminants from the ground surface to the tapping structure. This vulnerability assessment approach do not consider contaminant properties (intrinsic vulnerability) although it can be easily improved for evaluating the specific groundwater vulnerability. This concept of the sanitary protection zones was applied at two different type of aquifers: karstic aquifer of catchment area of Blederija springs and "Beli Timok" source of intergranular shallow aquifer. The first one represents a typical karst hydrogeological system with part of the catchment with allogenic recharge, and the second one, the groundwater source within shallow intergranular alluvial aquifer, dominantly recharged by river bank filtration. For sanitary protection zones delineation, the applied method has shown the importance of introducing all travel time components equally. In the case of the karstic source, the importance of the surface flow toward ponor zones has been emphasized, as a consequence of rapid travel time of water in relation to diffuse infiltration from autogenic part. When it comes to the shallow intergranular aquifer, the character of the unsaturated zone gets more prominent role in the source protection, as important buffer of the vertical movement downward. The applicability of proposed method has been shown regardless of the type of the aquifer, and at the same time intelligible results of the delineated sanitary protection zones are possible to validate with various methods. Key words: groundwater protection zoning, time dependent model, karst aquifer, intergranular aquifer, groundwater source protection

The modifiable areal unit problem (MAUP) in the relationship between exposure to NO2 and respiratory health

PubMed Central

2011-01-01

Background Many Canadian population health studies, including those focusing on the relationship between exposure to air pollution and health, have operationalized neighbourhoods at the census tract scale. At the same time, the conceptualization of place at the local scale is one of the weakest theoretical aspects in health geography. The modifiable areal unit problem (MAUP) raises issues when census tracts are used as neighbourhood proxies, and no other alternate spatial structure is used for sensitivity analysis. In the literature, conclusions on the relationship between NO2 and health outcomes are divided, and this situation may in part be due to the selection of an inappropriate spatial structure for analysis. Here, we undertake an analysis of NO2 and respiratory health in Ottawa, Canada using three different spatial structures in order to elucidate the effects that the spatial unit of analysis can have on analytical results. Results Using three different spatial structures to examine and quantify the relationship between NO2 and respiratory morbidity, we offer three main conclusions: 1) exploratory spatial analytical methods can serve as an indication of the potential effect of the MAUP; 2) OLS regression results differ significantly using different spatial representations, and this could be a contributing factor to the lack of consensus in studies that focus on the relation between NO2 and respiratory health at the area-level; and 3) the use of three spatial representations confirms no measured effect of NO2 exposure on respiratory health in Ottawa. Conclusions Area units used in population health studies should be delineated so as to represent the a priori scale of the expected scale interaction between neighbourhood processes and health. A thorough understanding of the role of the MAUP in the study of the relationship between NO2 and respiratory health is necessary for research into disease pathways based on statistical models, and for decision-makers to assess the scale at which interventions will have maximum benefit. In general, more research on the role of spatial representation in health studies is needed. PMID:22040001

Natural history of rhizomelic chondrodysplasia punctata.

PubMed

White, Amy L; Modaff, Peggy; Holland-Morris, Francesca; Pauli, Richard M

2003-05-01

Rhizomelic chondrodysplasia punctata (RCP) is a rare autosomal recessive disorder with many associated medical complications. Prior to this study, natural history information about RCP was limited and based on experiences with small populations of affected individuals. We delineate the natural history of RCP through systematic analysis of 35 previously unreported individuals (as well as review of 62 literature cases with respect to survival and cause of death). Survival, growth, and developmental expectations and medical needs are summarized based upon experience with this population. Survival is greater among this population than previously reported, with 90% surviving up to 1 year and 50% surviving up to 6 years. Cause of death is most often respiratory problem. All infants with RCP have joint contractures, bilateral cataracts, and severe growth and psychomotor delays. Recommendations for health supervision of children with RCP and for parental counseling are presented. Copyright 2003 Wiley-Liss, Inc.

Molecular insights into the biology of Greater Sage-Grouse

USGS Publications Warehouse

Oyler-McCance, Sara J.; Quinn, Thomas W.

2011-01-01

Recent research on Greater Sage-Grouse (Centrocercus urophasianus) genetics has revealed some important findings. First, multiple paternity in broods is more prevalent than previously thought, and leks do not comprise kin groups. Second, the Greater Sage-Grouse is genetically distinct from the congeneric Gunnison sage-grouse (C. minimus). Third, the Lyon-Mono population in the Mono Basin, spanning the border between Nevada and California, has unique genetic characteristics. Fourth, the previous delineation of western (C. u. phaios) and eastern Greater Sage-Grouse (C. u. urophasianus) is not supported genetically. Fifth, two isolated populations in Washington show indications that genetic diversity has been lost due to population declines and isolation. This chapter examines the use of molecular genetics to understand the biology of Greater Sage-Grouse for the conservation and management of this species and put it into the context of avian ecology based on selected molecular studies.

Enhancing palliative care delivery in a regional community in Australia.

PubMed

Phillips, Jane L; Davidson, Patricia M; Jackson, Debra; Kristjanson, Linda; Bennett, Margaret L; Daly, John

2006-08-01

Although access to palliative care is a fundamental right for people in Australia and is endorsed by government policy, there is often limited access to specialist palliative care services in regional, rural and remote areas. This article appraises the evidence pertaining to palliative care service delivery to inform a sustainable model of palliative care that meets the needs of a regional population on the mid-north coast of New South Wales. Expert consultation and an eclectic literature review were undertaken to develop a model of palliative care service delivery appropriate to the needs of the target population and resources of the local community. On the basis of this review, a local palliative care system that is based on a population-based approach to service planning and delivery, with formalized integrated network agreements and role delineation between specialist and generalist providers, has the greatest potential to meet the palliative care needs of this regional coastal community.

Application of LANDSAT satellite imagery for iron ore prospecting in the Western Desert of Egypt

NASA Technical Reports Server (NTRS)

Elshazly, E. M.; Abdelhady, M. A.; Elghawaby, M. A.; Khawasik, S. M.

1977-01-01

Prospecting for iron ore occurrences was conducted by the Remote Sensing Center in Bahariya Oasis-El Faiyum area covering some 100,000 km squared in the Western Desert of Egypt. LANDSAT-1 satellite images were utilized as the main tool in the regional prospecting of the iron ores. The delineation of the geological units and geological structure through the interpretation of the images corroborated by field observations and structural analysis led to the discovery of new iron ore occurrences in the area of investigation.

Proposed structural basis of interaction of piperine and related compounds with monoamine oxidases.

PubMed

Rahman, Taufiq; Rahmatullah, Mohammed

2010-01-15

Several studies have revealed piperine and a few related compounds as potent inhibitors of monoamine oxidases without delineating the underlying mechanism. Using in silico modelling, we propose a structural basis of such activity by showing that these compounds can successfully dock into the inhibitor binding pockets of human monoamine oxidase isoforms with predicted affinities comparable to some known inhibitors. The results therefore suggest that piperine can be a promising lead for developing novel monoamine oxidase inhibitors. Copyright 2009 Elsevier Ltd. All rights reserved.

3D automatic anatomy recognition based on iterative graph-cut-ASM

NASA Astrophysics Data System (ADS)

Chen, Xinjian; Udupa, Jayaram K.; Bagci, Ulas; Alavi, Abass; Torigian, Drew A.

2010-02-01

We call the computerized assistive process of recognizing, delineating, and quantifying organs and tissue regions in medical imaging, occurring automatically during clinical image interpretation, automatic anatomy recognition (AAR). The AAR system we are developing includes five main parts: model building, object recognition, object delineation, pathology detection, and organ system quantification. In this paper, we focus on the delineation part. For the modeling part, we employ the active shape model (ASM) strategy. For recognition and delineation, we integrate several hybrid strategies of combining purely image based methods with ASM. In this paper, an iterative Graph-Cut ASM (IGCASM) method is proposed for object delineation. An algorithm called GC-ASM was presented at this symposium last year for object delineation in 2D images which attempted to combine synergistically ASM and GC. Here, we extend this method to 3D medical image delineation. The IGCASM method effectively combines the rich statistical shape information embodied in ASM with the globally optimal delineation capability of the GC method. We propose a new GC cost function, which effectively integrates the specific image information with the ASM shape model information. The proposed methods are tested on a clinical abdominal CT data set. The preliminary results show that: (a) it is feasible to explicitly bring prior 3D statistical shape information into the GC framework; (b) the 3D IGCASM delineation method improves on ASM and GC and can provide practical operational time on clinical images.

Hippocampus in health and disease: An overview

PubMed Central

Anand, Kuljeet Singh; Dhikav, Vikas

2012-01-01

Hippocampus is a complex brain structure embedded deep into temporal lobe. It has a major role in learning and memory. It is a plastic and vulnerable structure that gets damaged by a variety of stimuli. Studies have shown that it also gets affected in a variety of neurological and psychiatric disorders. In last decade or so, lot has been learnt about conditions that affect hippocampus and produce changes ranging from molecules to morphology. Progresses in radiological delineation, electrophysiology, and histochemical characterization have made it possible to study this archicerebral structure in greater detail. Present paper attempts to give an overview of hippocampus, both in health and diseases. PMID:23349586

Segmentation of heterogeneous blob objects through voting and level set formulation

PubMed Central

Chang, Hang; Yang, Qing; Parvin, Bahram

2009-01-01

Blob-like structures occur often in nature, where they aid in cueing and the pre-attentive process. These structures often overlap, form perceptual boundaries, and are heterogeneous in shape, size, and intensity. In this paper, voting, Voronoi tessellation, and level set methods are combined to delineate blob-like structures. Voting and subsequent Voronoi tessellation provide the initial condition and the boundary constraints for each blob, while curve evolution through level set formulation provides refined segmentation of each blob within the Voronoi region. The paper concludes with the application of the proposed method to a dataset produced from cell based fluorescence assays and stellar data. PMID:19774202

Use of Maximum Intensity Projections (MIPs) for target outlining in 4DCT radiotherapy planning.

PubMed

Muirhead, Rebecca; McNee, Stuart G; Featherstone, Carrie; Moore, Karen; Muscat, Sarah

2008-12-01

Four-dimensional computed tomography (4DCT) is currently being introduced to radiotherapy centers worldwide, for use in radical radiotherapy planning for non-small cell lung cancer (NSCLC). A significant drawback is the time required to delineate 10 individual CT scans for each patient. Every department will hence ask the question if the single Maximum Intensity Projection (MIP) scan can be used as an alternative. Although the problems regarding the use of the MIP in node-positive disease have been discussed in the literature, a comprehensive study assessing its use has not been published. We compared an internal target volume (ITV) created using the MIP to an ITV created from the composite volume of 10 clinical target volumes (CTVs) delineated on the 10 phases of the 4DCT. 4DCT data was collected from 14 patients with NSCLC. In each patient, the ITV was delineated on the MIP image (ITV_MIP) and a composite ITV created from the 10 CTVs delineated on each of the 10 scans in the dataset. The structures were compared by assessment of volumes of overlap and exclusion. There was a median of 19.0% (range, 5.5-35.4%) of the volume of ITV_10phase not enclosed by the ITV_MIP, demonstrating that the use of the MIP could result in under-treatment of disease. In contrast only a very small amount of the ITV_MIP was not enclosed by the ITV_10phase (median of 2.3%, range, 0.4-9.8%), indicating the ITV_10phase covers almost all of the tumor tissue as identified by MIP. Although there were only two Stage I patients, both demonstrated very similar ITV_10phase and ITV_MIP volumes. These findings suggest that Stage I NSCLC tumors could be outlined on the MIP alone. In Stage II and III tumors the ITV_10phase would be more reliable. To prevent under-treatment of disease, the MIP image can only be used for delineation in Stage I tumors.

Population structure of guppies in north-eastern Venezuela, the area of putative incipient speciation

PubMed Central

2014-01-01

Background Geographic barriers to gene flow and divergence among populations in sexual traits are two important causes of genetic isolation which may lead to speciation. Genetic isolation may be facilitated if these two mechanisms act synergistically. The guppy from the Cumaná region (within the Cariaco drainage) of eastern Venezuela has been previously described as a case of incipient speciation driven by sexual selection, significantly differentiated in sexual colouration and body shape from the common guppy, Poecilia reticulata. The latter occurs widely in northern Venezuela, including the south-eastern side of Cordillera de la Costa, where it inhabits streams belonging to the San Juan drainage. Here, we present molecular and morphological analyses of differentiation among guppy populations in the Cariaco and San Juan drainages. Our analyses are based on a 953 bp long mtDNA fragment, a set of 15 microsatellites (519 fish from 20 populations), and four phenotypic traits. Results Both microsatellite and mtDNA data showed that guppies inhabiting the two drainages are characterised by a significant genetic differentiation, but a higher proportion of the genetic variance was distributed among populations within regions. Most guppies in the Cariaco drainage had mtDNA from a distinct lineage, but we also found evidence for widespread introgression of mtDNA from the San Juan drainage into the Cariaco drainage. Phenotypically, populations in the two regions differed significantly only in the number of black crescents. Phenotypic clustering did not support existence of two distinct groupings, but indicated a degree of distinctiveness of Central Cumaná (CC) population. However, CC population showed little differentiation at the neutral markers from the proximate populations within the Cariaco drainage. Conclusions Our findings are consistent with only partial genetic isolation between the two geographic regions and indicate that the geographic barrier of Cordillera de la Costa has not played an important role in strengthening the incomplete pre-zygotic reproductive barrier between Cumaná and common guppy. Significant phenotypic differentiation between genetically similar (in terms of neutral variation) populations suggests that mate choice can maintain divergence at sexually selected traits despite gene flow. However, neither genetic nor phenotypic clustering supported delineation of two species within the region. PMID:24533965

Interactive Cadastral Boundary Delineation from Uav Data

NASA Astrophysics Data System (ADS)

Crommelinck, S.; Höfle, B.; Koeva, M. N.; Yang, M. Y.; Vosselman, G.

2018-05-01

Unmanned aerial vehicles (UAV) are evolving as an alternative tool to acquire land tenure data. UAVs can capture geospatial data at high quality and resolution in a cost-effective, transparent and flexible manner, from which visible land parcel boundaries, i.e., cadastral boundaries are delineable. This delineation is to no extent automated, even though physical objects automatically retrievable through image analysis methods mark a large portion of cadastral boundaries. This study proposes (i) a methodology that automatically extracts and processes candidate cadastral boundary features from UAV data, and (ii) a procedure for a subsequent interactive delineation. Part (i) consists of two state-of-the-art computer vision methods, namely gPb contour detection and SLIC superpixels, as well as a classification part assigning costs to each outline according to local boundary knowledge. Part (ii) allows a user-guided delineation by calculating least-cost paths along previously extracted and weighted lines. The approach is tested on visible road outlines in two UAV datasets from Germany. Results show that all roads can be delineated comprehensively. Compared to manual delineation, the number of clicks per 100 m is reduced by up to 86 %, while obtaining a similar localization quality. The approach shows promising results to reduce the effort of manual delineation that is currently employed for indirect (cadastral) surveying.

Applicability of radon emanometry in lithologically discontinuous sites contaminated by organic chemicals.

PubMed

De Miguel, Eduardo; Barrio-Parra, Fernando; Elío, Javier; Izquierdo-Díaz, Miguel; García-González, Jerónimo Emilio; Mazadiego, Luis Felipe; Medina, Rafael

2018-06-02

The applicability of radon ( 222 Rn) measurements to delineate non-aqueous phase liquids (NAPL) contamination in subsoil is discussed at a site with lithological discontinuities through a blind test. Three alpha spectroscopy monitors were used to measure radon in soil air in a 25,000-m 2 area, following a regular sampling design with a 20-m 2 grid. Repeatability and reproducibility of the results were assessed by means of duplicate measurements in six sampling positions. Furthermore, three points not affected by oil spills were sampled to estimate radon background concentration in soil air. Data histograms, Q-Q plots, variograms, and cluster analysis allowed to recognize two data populations, associated with the possible path of a fault and a lithological discontinuity. Even though the concentration of radon in soil air was dominated by this discontinuity, the characterization of the background emanation in each lithological unit allowed to distinguish areas potentially affected by NAPL, thus justifying the application of radon emanometry as a screening technique for the delineation of NAPL plumes in sites with lithological discontinuities.

Volcanic calderas delineate biogeographic provinces among Yellowstone thermophiles.

PubMed

Takacs-Vesbach, Cristina; Mitchell, Kendra; Jackson-Weaver, Olan; Reysenbach, Anna-Louise

2008-07-01

It has been suggested that the distribution of microorganisms should be cosmopolitan because of their enormous capacity for dispersal. However, recent studies have revealed that geographically isolated microbial populations do exist. Geographic distance as a barrier to dispersal is most often invoked to explain these distributions. Here we show that unique and diverse sequences of the bacterial genus Sulfurihydrogenibium exist in Yellowstone thermal springs, indicating that these sites are geographically isolated. Although there was no correlation with geographic distance or the associated geochemistry of the springs, there was a strong historical signal. We found that the Yellowstone calderas, remnants of prehistoric volcanic eruptions, delineate biogeographical provinces for the Sulfurihydrogenibium within Yellowstone (chi(2): 9.7, P = 0.002). The pattern of distribution that we have detected suggests that major geological events in the past 2 million years explain more of the variation in sequence diversity in this system than do contemporary factors such as habitat or geographic distance. These findings highlight the importance of historical legacies in determining contemporary microbial distributions and suggest that the same factors that determine the biogeography of macroorganisms are also evident among bacteria.

(I Can't Get No) Saturation: A simulation and guidelines for sample sizes in qualitative research.

PubMed

van Rijnsoever, Frank J

2017-01-01

I explore the sample size in qualitative research that is required to reach theoretical saturation. I conceptualize a population as consisting of sub-populations that contain different types of information sources that hold a number of codes. Theoretical saturation is reached after all the codes in the population have been observed once in the sample. I delineate three different scenarios to sample information sources: "random chance," which is based on probability sampling, "minimal information," which yields at least one new code per sampling step, and "maximum information," which yields the largest number of new codes per sampling step. Next, I use simulations to assess the minimum sample size for each scenario for systematically varying hypothetical populations. I show that theoretical saturation is more dependent on the mean probability of observing codes than on the number of codes in a population. Moreover, the minimal and maximal information scenarios are significantly more efficient than random chance, but yield fewer repetitions per code to validate the findings. I formulate guidelines for purposive sampling and recommend that researchers follow a minimum information scenario.

Using color and grayscale images to teach histology to color-deficient medical students.

PubMed

Rubin, Lindsay R; Lackey, Wendy L; Kennedy, Frances A; Stephenson, Robert B

2009-01-01

Examination of histologic and histopathologic microscopic sections relies upon differential colors provided by staining techniques, such as hematoxylin and eosin, to delineate normal tissue components and to identify pathologic alterations in these components. Given the prevalence of color deficiency (commonly called "color blindness") in the general population, it is likely that this reliance upon color differentiation poses a significant obstacle for several medical students beginning a course of study that includes examination of histologic slides. In the past, first-year medical students at Michigan State University who identified themselves as color deficient were encouraged to use color transparency overlays or tinted contact lenses to filter out problematic colors. Recently, however, we have offered such students a computer monitor adjusted to grayscale for in-lab work, as well as grayscale copies of color photomicrographs for examination purposes. Grayscale images emphasize the texture of tissues and the contrasts between tissues as the students learn histologic architecture. Using this approach, color-deficient students have quickly learned to compensate for their deficiency by focusing on cell and tissue structure rather than on color variation. Based upon our experience with color-deficient students, we believe that grayscale photomicrographs may also prove instructional for students with normal (trichromatic) color vision, by encouraging them to consider structural characteristics of cells and tissues that may otherwise be overshadowed by stain colors.

Single-cell paired-end genome sequencing reveals structural variation per cell cycle

PubMed Central

Voet, Thierry; Kumar, Parveen; Van Loo, Peter; Cooke, Susanna L.; Marshall, John; Lin, Meng-Lay; Zamani Esteki, Masoud; Van der Aa, Niels; Mateiu, Ligia; McBride, David J.; Bignell, Graham R.; McLaren, Stuart; Teague, Jon; Butler, Adam; Raine, Keiran; Stebbings, Lucy A.; Quail, Michael A.; D’Hooghe, Thomas; Moreau, Yves; Futreal, P. Andrew; Stratton, Michael R.; Vermeesch, Joris R.; Campbell, Peter J.

2013-01-01

The nature and pace of genome mutation is largely unknown. Because standard methods sequence DNA from populations of cells, the genetic composition of individual cells is lost, de novo mutations in cells are concealed within the bulk signal and per cell cycle mutation rates and mechanisms remain elusive. Although single-cell genome analyses could resolve these problems, such analyses are error-prone because of whole-genome amplification (WGA) artefacts and are limited in the types of DNA mutation that can be discerned. We developed methods for paired-end sequence analysis of single-cell WGA products that enable (i) detecting multiple classes of DNA mutation, (ii) distinguishing DNA copy number changes from allelic WGA-amplification artefacts by the discovery of matching aberrantly mapping read pairs among the surfeit of paired-end WGA and mapping artefacts and (iii) delineating the break points and architecture of structural variants. By applying the methods, we capture DNA copy number changes acquired over one cell cycle in breast cancer cells and in blastomeres derived from a human zygote after in vitro fertilization. Furthermore, we were able to discover and fine-map a heritable inter-chromosomal rearrangement t(1;16)(p36;p12) by sequencing a single blastomere. The methods will expedite applications in basic genome research and provide a stepping stone to novel approaches for clinical genetic diagnosis. PMID:23630320

Area 4 has layer IV in adult primates

PubMed Central

García-Cabezas, Miguel Ángel; Barbas, Helen

2014-01-01

There are opposing views about the status of layer IV in primary motor cortex (area 4). Cajal described a layer IV in area 4 of adult humans. In contrast, Brodmann found layer IV in development but not in adult primates and called area 4 ‘agranular’. We addressed this issue in rhesus monkeys using the neural marker SMI-32, which labels neurons in lower layer III and upper V, but not in layer IV. SMI-32 delineated a central unlabeled cortical stripe in area 4 that corresponds to layer IV, which was populated with small interneurons also found in layer IV in ‘granular’ areas (such as area 46). We distinguished layer IV interneurons from projection neurons in the layers above and below using cellular criteria. The commonly used term ‘agranular’ for area 4 is also used for the phylogenetically ancient limbic cortices, confusing areas that differ markedly in laminar structure. This issue pertains to the systematic variation in the architecture across cortices, traced from limbic cortices through areas with increasingly more elaborate laminar structure. The principle of systematic variation can be used to predict laminar patterns of connections across cortical systems. This principle places area 4 and agranular anterior cingulate cortices at opposite poles of the graded laminar differentiation of motor cortices. The status of layer IV in area 4 thus pertains to core organizational features of the cortex, its connections and evolution. PMID:24735460

Experimental use of temporary traffic delineators : final report.

DOT National Transportation Integrated Search

1988-12-01

During construction, there is a safety need to provide highly visible channelizing delineation for temporary protection and direction of traffic. "Super Duck" Surface Mounted Delineator (SDR-328, Rounded) by Carsonite is marked to meet the safety nee...

California Groundwater Units

USGS Publications Warehouse

Johnson, Tyler D.; Belitz, Kenneth

2014-01-01

The California Groundwater Units dataset classifies and delineates areas within the State of California into one of three groundwater-based polygon units: (1) those areas previously defined as alluvial groundwater basins or subbasins, (2) highland areas that are adjacent to and topographically upgradient of groundwater basins, and (3) highland areas not associated with a groundwater basin, only a hydrogeologic province. In total, 938 Groundwater Units are represented. The Groundwater Units dataset relates existing groundwater basins with their newly delineated highland areas which can be used in subsequent hydrologic studies. The methods used to delineate groundwater-basin-associated highland areas are similar to those used to delineate a contributing area (such as for a lake or water body); the difference is that highland areas are constrained to the immediately surrounding upslope (upstream) area. Upslope basins have their own delineated highland. A geoprocessing tool was created to facilitate delineation of highland areas for groundwater basins and subbasins and is available for download.

Geology of the Cape Mendocino, Eureka, Garberville, and Southwestern Part of the Hayfork 30 x 60 Minute Quadrangles and Adjacent Offshore Area, Northern California

USGS Publications Warehouse

McLaughlin, Robert J.; Ellen, S.D.; Blake, M.C.; Jayko, Angela S.; Irwin, W.P.; Aalto, K.R.; Carver, G.A.; Clarke, S.H.; Barnes, J.B.; Cecil, J.D.; Cyr, K.A.

2000-01-01

Introduction These geologic maps and accompanying structure sections depict the geology and structure of much of northwestern California and the adjacent continental margin. The map area includes the Mendocino triple junction, which is the juncture of the North American continental plate with two plates of the Pacific ocean basin. The map area also encompasses major geographic and geologic provinces of northwestern California. The maps incorporate much previously unpublished geologic mapping done between 1980 and 1995, as well as published mapping done between about 1950 and 1978. To construct structure sections to mid-crustal depths, we integrate the surface geology with interpretations of crustal structure based on seismicity, gravity and aeromagnetic data, offshore structure, and seismic reflection and refraction data. In addition to describing major geologic and structural features of northwestern California, the geologic maps have the potential to address a number of societally relevant issues, including hazards from earthquakes, landslides, and floods and problems related to timber harvest, wildlife habitat, and changing land use. All of these topics will continue to be of interest in the region, as changing land uses and population density interact with natural conditions. In these interactions, it is critical that the policies and practices affecting man and the environment integrate an adequate understanding of the geology. This digital map database, compiled from previously published and unpublished data, and new mapping by the authors, represents the general distribution of bedrock and surficial deposits in the mapped area. Together with the accompanying text file (ceghmf.ps, ceghmf.pdf, ceghmf.txt), it provides current information on the geologic structure and stratigraphy of the area covered. The database delineates map units that are identified by general age and lithology following the stratigraphic nomenclature of the U.S. Geological Survey. The scale of the source maps limits the spatial resolution (scale) of the database to 1:100,000 or smaller.

Application of Carbonate Reservoir using waveform inversion and reverse-time migration methods

NASA Astrophysics Data System (ADS)

Kim, W.; Kim, H.; Min, D.; Keehm, Y.

2011-12-01

Recent exploration targets of oil and gas resources are deeper and more complicated subsurface structures, and carbonate reservoirs have become one of the attractive and challenging targets in seismic exploration. To increase the rate of success in oil and gas exploration, it is required to delineate detailed subsurface structures. Accordingly, migration method is more important factor in seismic data processing for the delineation. Seismic migration method has a long history, and there have been developed lots of migration techniques. Among them, reverse-time migration is promising, because it can provide reliable images for the complicated model even in the case of significant velocity contrasts in the model. The reliability of seismic migration images is dependent on the subsurface velocity models, which can be extracted in several ways. These days, geophysicists try to obtain velocity models through seismic full waveform inversion. Since Lailly (1983) and Tarantola (1984) proposed that the adjoint state of wave equations can be used in waveform inversion, the back-propagation techniques used in reverse-time migration have been used in waveform inversion, which accelerated the development of waveform inversion. In this study, we applied acoustic waveform inversion and reverse-time migration methods to carbonate reservoir models with various reservoir thicknesses to examine the feasibility of the methods in delineating carbonate reservoir models. We first extracted subsurface material properties from acoustic waveform inversion, and then applied reverse-time migration using the inverted velocities as a background model. The waveform inversion in this study used back-propagation technique, and conjugate gradient method was used in optimization. The inversion was performed using the frequency-selection strategy. Finally waveform inversion results showed that carbonate reservoir models are clearly inverted by waveform inversion and migration images based on the inversion results are quite reliable. Different thicknesses of reservoir models were also described and the results revealed that the lower boundary of the reservoir was not delineated because of energy loss. From these results, it was noted that carbonate reservoirs can be properly imaged and interpreted by waveform inversion and reverse-time migration methods. This work was supported by the Energy Resources R&D program of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant funded by the Korea government Ministry of Knowledge Economy (No. 2009201030001A, No. 2010T100200133) and the Brain Korea 21 project of Energy System Engineering.

WE-G-BRD-04: BEST IN PHYSICS (JOINT IMAGING-THERAPY): An Integrated Model-Based Intrafractional Organ Motion Tracking Approach with Dynamic MRI in Head and Neck Radiotherapy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chen, H; Dolly, S; Anastasio, M

Purpose: In-treatment dynamic cine images, provided by the first commercially available MRI-guided radiotherapy system, allow physicians to observe intrafractional motion of head and neck (H&N) internal structures. Nevertheless, high anatomical complexity and relatively poor cine image contrast/resolution have complicated automatic intrafractional motion evaluation. We proposed an integrated model-based approach to automatically delineate and analyze moving structures from on-board cine images. Methods: The H&N upper airway, a complex and highly deformable region wherein severe internal motion often occurs, was selected as the target-to-be-tracked. To reliably capture its motion, a hierarchical structure model containing three statistical shapes (face, face-jaw, and face-jaw-palate) wasmore » first built from a set of manually delineated shapes using principal component analysis. An integrated model-fitting algorithm was then employed to align the statistical shapes to the first to-be-detected cine frame, and multi-feature level-set contour propagation was performed to identify the airway shape change in the remaining frames. Ninety sagittal cine MR image sets, acquired from three H&N cancer patients, were utilized to demonstrate this approach. Results: The tracking accuracy was validated by comparing the results to the average of two manual delineations in 20 randomly selected images from each patient. The resulting dice similarity coefficient (93.28+/−1.46 %) and margin error (0.49+/−0.12 mm) showed good agreement with the manual results. Intrafractional displacements of anterior, posterior, inferior, and superior airway boundaries were observed, with values of 2.62+/−2.92, 1.78+/−1.43, 3.51+/−3.99, and 0.68+/−0.89 mm, respectively. The H&N airway motion was found to vary across directions, fractions, and patients, and highly correlated with patients’ respiratory frequency. Conclusion: We proposed the integrated computational approach, which for the first time allows to automatically identify the H&N upper airway and quantify in-treatment H&N internal motion in real-time. This approach can be applied to track other structures’ motion, and provide guidance on patient-specific prediction of intra-/inter-fractional structure displacements.« less

Properties of Self-Assembled Monolayers Revealed via Inverse Tensiometry.

PubMed

Chen, Jiahao; Wang, Zhengjia; Oyola-Reynoso, Stephanie; Thuo, Martin M

2017-11-28

Self-assembled monolayers (SAMs) have emerged as a simple platform technology and hence have been broadly studied. With advances in state-of-the-art fabrication and characterization methods, new insights into SAM structure and related properties have been delineated, albeit with some discrepancies and/or incoherencies. Some discrepancies, especially between experimental and theoretical work, are in part due to the misunderstanding of subtle structural features such as phase evolution and SAM quality. Recent work has, however, shown that simple techniques, such as the measurement of static contact angles, can be used to delineate otherwise complex properties of the SAM, especially when complemented by other more advanced techniques. In this article, we highlight the effect of nanoscale substrate asperities and molecular chain length on the SAM structure and associated properties. First, surfaces with tunable roughness are prepared on both Au and Ag, and their corresponding n-alkanethiolate SAMs are characterized through wetting and spectroscopy. From these data, chain-length- and substrate-morphology-dependent limits to the odd-even effect (structure and properties vary with the number of carbons in the molecules and the nature of the substrate), parametrization of gauche defect densities, and structural phase evolution (liquidlike, waxy, crystalline interfaces) are deduced. An evaluation of the correlation between the effect of roughness and the components of surface tension (polar-γ p and dispersive-γ d ) reveals that wetting, at nanoscale rough surfaces, evolves proportionally with the ratio of the two components of surface tension. The evolution of conformational order is captured over a range of molecular lengths and parametrized through a dimensionless number, χ c . By deploying a well-known tensiometry technique (herein the liquid is used to characterize the solid, hence the term inverse tensiometry) to characterize SAMs, we demonstrate that complex molecular-level phenomena in SAMs can be understood through simplicity.

Triterpenoid glycosides from Bacopa monnieri.

PubMed

Sivaramakrishna, Chillara; Rao, Chirravuri V; Trimurtulu, Golakoti; Vanisree, Mulabagal; Subbaraju, Gottumukkala V

2005-12-01

Two triterpenoid glycosides have been isolated along with 10 known saponins from Bacopa monnieri. Structures of the compounds have been elucidated as 3-O-[beta-D-glucopyranosyl-(1-->3)-beta-D-glucopyranosyl] jujubogenin (1) and 3-O-[beta-D-glucopyranosyl-(1-->3)-beta-D-glucopyranosyl] pseudojujubogenin (2) by high resolution NMR spectral data and chemical correlations. Further, the chemical compositions of bacosides A and B have been delineated.

Medieval Europe. Grade 7 Model Lesson for Standard 7.6. World History and Geography: Medieval and Early Modern Times. California History-Social Science Course Models.

ERIC Educational Resources Information Center

Zachlod, Michelle, Ed.

California State Standard 7.6 is delineated in the following manner: "Students analyze the geographic, political, economic, religious, and social structures of the civilizations of Medieval Europe." Seventh-grade students study the geography of Europe and the Eurasian land mass; describe the spread of Christianity north of the Alps and…

Laser Covariance Vibrometry for Unsymmetrical Mode Detection

DTIC Science & Technology

2006-09-01

surface rough- ness. Results show that the remote sensing spectra adequately match the structural vibration, including non – imaging spatially...the speckle. 10 profile (cross – section), is an air turbulence effect ignored in this work that will affect both the sensed vibration phase change and...like spike impulse. 13 Chapter three describes optical processing issues. This chapter delineates the image propagation algorithms used for the work

Medieval Japan. Grade 7 Model Lesson for Standard 7.5. World History and Geography: Medieval and Early Modern Times. California History-Social Science Course Models.

ERIC Educational Resources Information Center

Zachlod, Michelle, Ed.

California State Standard 7.5 is delineated in the following manner: "Students analyze the geographic, political, economic, religious, and social structures of medieval Japan." Seventh-grade students describe the significance of Japan's proximity to China and Korea and the influence of these countries on Japan; discuss the reign of…

Computer-based synthetic data to assess the tree delineation algorithm from airborne LiDAR survey

Treesearch

Lei Wang; Andrew G. Birt; Charles W. Lafon; David M. Cairns; Robert N. Coulson; Maria D. Tchakerian; Weimin Xi; Sorin C. Popescu; James M. Guldin

2013-01-01

Small Footprint LiDAR (Light Detection And Ranging) has been proposed as an effective tool for measuring detailed biophysical characteristics of forests over broad spatial scales. However, by itself LiDAR yields only a sample of the true 3D structure of a forest. In order to extract useful forestry relevant information, this data must be interpreted using mathematical...

Development of a preoperative simulation technique for carotid endarterectomy in patients with contrast contraindications.

PubMed

Nomura, Shunsuke; Hayashi, Motohiro; Ishikawa, Tatsuya; Yamaguchi, Koji; Kawamata, Takakazu

2018-05-19

Vascular and osteological parameters, such as the heights of the carotid bifurcation and distal end of the plaque, are important preoperative considerations for patients undergoing carotid stenosis procedures such as carotid endarterectomy. However, for patients with contrast contraindications such as allergies or nephropathies, three-dimensional computed tomography angiography (3D-CTA) is unavailable, and preoperative evaluation remains challenging. In the present study, we aimed to develop a preoperative simulation for use in patients with contrast-contraindicated carotid stenosis. Images from non-contrast neck CT and magnetic resonance imaging obtained without the Leksell stereotactic frame were uploaded to GammaPlan. Following delineation of various structures, we performed preoperative simulations to determine the relationships between vascular and osteological structures. We applied this technique in 10 patients with carotid stenosis to verify the accuracy of the simulation. In all patients, the GammaPlan simulation successfully visualized the heights of the carotid bifurcation and distal end of the plaque without the use of contrast medium. Furthermore, information regarding the location of internal arterial structures, such as calcifications and unstable plaques, could be incorporated into GammaPlan images. Thereafter, we verified simulation accuracy by comparing the simulation results with 3D-CTA and operative findings. Simulations created using GammaPlan can be used to obtain accurate vascular and osteological information regarding the heights of the carotid bifurcation and distal end of the plaque, without the use of contrast medium. The reconstruction of delineated structures using this technique may be effective for preoperative evaluation in patients with contrast-contraindicated carotid stenosis. Copyright © 2018 Elsevier Inc. All rights reserved.

Slic Superpixels for Object Delineation from Uav Data

NASA Astrophysics Data System (ADS)

Crommelinck, S.; Bennett, R.; Gerke, M.; Koeva, M. N.; Yang, M. Y.; Vosselman, G.

2017-08-01

Unmanned aerial vehicles (UAV) are increasingly investigated with regard to their potential to create and update (cadastral) maps. UAVs provide a flexible and low-cost platform for high-resolution data, from which object outlines can be accurately delineated. This delineation could be automated with image analysis methods to improve existing mapping procedures that are cost, time and labor intensive and of little reproducibility. This study investigates a superpixel approach, namely simple linear iterative clustering (SLIC), in terms of its applicability to UAV data. The approach is investigated in terms of its applicability to high-resolution UAV orthoimages and in terms of its ability to delineate object outlines of roads and roofs. Results show that the approach is applicable to UAV orthoimages of 0.05 m GSD and extents of 100 million and 400 million pixels. Further, the approach delineates the objects with the high accuracy provided by the UAV orthoimages at completeness rates of up to 64 %. The approach is not suitable as a standalone approach for object delineation. However, it shows high potential for a combination with further methods that delineate objects at higher correctness rates in exchange of a lower localization quality. This study provides a basis for future work that will focus on the incorporation of multiple methods for an interactive, comprehensive and accurate object delineation from UAV data. This aims to support numerous application fields such as topographic and cadastral mapping.

A multifactor analysis of fungal and bacterial community structure of the root microbiome of mature Populus deltoides trees

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shakya, Migun; Gottel, Neil R; Castro Gonzalez, Hector F

2013-01-01

Bacterial and fungal communities associated with plant roots are central to the host- health, survival and growth. However, a robust understanding of root-microbiome and the factors that drive host associated microbial community structure have remained elusive, especially in mature perennial plants from natural settings. Here, we investigated relationships of bacterial and fungal communities in the rhizosphere and root endosphere of the riparian tree species Populus deltoides, and the influence of soil parameters, environmental properties (host phenotype and aboveground environmental settings), host plant genotype (Simple Sequence Repeat (SSR) markers), season (Spring vs. Fall) and geographic setting (at scales from regional watershedsmore » to local riparian zones) on microbial community structure. Each of the trees sampled displayed unique aspects to it s associated community structure with high numbers of Operational Taxonomic Units (OTUs) specific to an individual trees (bacteria >90%, fungi >60%). Over the diverse conditions surveyed only a small number of OTUs were common to all samples within rhizosphere (35 bacterial and 4 fungal) and endosphere (1 bacterial and 1 fungal) microbiomes. As expected, Proteobacteria and Ascomycota were dominant in root communities (>50%) while other higher-level phylogenetic groups (Chytridiomycota, Acidobacteria) displayed greatly reduced abundance in endosphere compared to the rhizosphere. Variance partitioning partially explained differences in microbiome composition between all sampled roots on the basis of seasonal and soil properties (4% to 23%). While most variation remains unattributed, we observed significant differences in the microbiota between watersheds (Tennessee vs. North Carolina) and seasons (Spring vs. Fall). SSR markers clearly delineated two host populations associated with the samples taken in TN vs. NC, but overall genotypic distances did not have a significant effect on corresponding communities that could be separated from other measured effects.« less

A Multifactor Analysis of Fungal and Bacterial Community Structure in the Root Microbiome of Mature Populus deltoides Trees

PubMed Central

Shakya, Migun; Gottel, Neil; Castro, Hector; Yang, Zamin K.; Gunter, Lee; Labbé, Jessy; Muchero, Wellington; Bonito, Gregory; Vilgalys, Rytas; Tuskan, Gerald; Podar, Mircea; Schadt, Christopher W.

2013-01-01

Bacterial and fungal communities associated with plant roots are central to the host health, survival and growth. However, a robust understanding of the root-microbiome and the factors that drive host associated microbial community structure have remained elusive, especially in mature perennial plants from natural settings. Here, we investigated relationships of bacterial and fungal communities in the rhizosphere and root endosphere of the riparian tree species Populus deltoides, and the influence of soil parameters, environmental properties (host phenotype and aboveground environmental settings), host plant genotype (Simple Sequence Repeat (SSR) markers), season (Spring vs. Fall) and geographic setting (at scales from regional watersheds to local riparian zones) on microbial community structure. Each of the trees sampled displayed unique aspects to its associated community structure with high numbers of Operational Taxonomic Units (OTUs) specific to an individual trees (bacteria >90%, fungi >60%). Over the diverse conditions surveyed only a small number of OTUs were common to all samples within rhizosphere (35 bacterial and 4 fungal) and endosphere (1 bacterial and 1 fungal) microbiomes. As expected, Proteobacteria and Ascomycota were dominant in root communities (>50%) while other higher-level phylogenetic groups (Chytridiomycota, Acidobacteria) displayed greatly reduced abundance in endosphere compared to the rhizosphere. Variance partitioning partially explained differences in microbiome composition between all sampled roots on the basis of seasonal and soil properties (4% to 23%). While most variation remains unattributed, we observed significant differences in the microbiota between watersheds (Tennessee vs. North Carolina) and seasons (Spring vs. Fall). SSR markers clearly delineated two host populations associated with the samples taken in TN vs. NC, but overall host genotypic distances did not have a significant effect on corresponding communities that could be separated from other measured effects. PMID:24146861

Improved business driveway delineation in urban work zones.

DOT National Transportation Integrated Search

2015-04-01

This report documents the efforts and results of a two-year research project aimed at improving driveway : delineation in work zones. The first year of the project included a closed-course study to identify the most : promising driveway delineation a...

Lifetime history of traumatic events in a young adult Mexican American sample: relation to substance dependence, affective disorder, acculturation stress, and PTSD

PubMed Central

Ehlers, Cindy L.; Kim, Corinne; Gilder, David A.; Stouffer, Gina M.; Caetano, Raul; Yehuda, Rachel

2016-01-01

Mexican Americans comprise one of the most rapidly growing populations in the United States, and within this population, trauma and post-traumatic stress disorder (PTSD) are associated with physical and mental health problems. Therefore, efforts to delineate factors that may uniquely contribute to increased likelihood of trauma, PTSD, and substance use disorders over the lifetime in Mexican Americans are important to address health disparities and to develop treatment and prevention programs. Six hundred fourteen young adults (age 18–30 yrs) of Mexican American heritage, largely second generation, were recruited from the community and assessed with the Semi-Structured Assessment for the Genetics of Alcoholism and an acculturation stress scale. More males (51.2%) reported experiencing traumas than females (41.1%), however, a larger proportion of females received a PTSD diagnosis (15%) than males (8%). Alcohol dependence and affective disorders, but not anxiety disorders, antisocial disorders, nicotine, marijuana, or stimulant dependence, were significantly comorbid with PTSD. Endorsing higher levels of acculturation stress was also significantly associated with both trauma exposure and a diagnosis of PTSD. Logistic regression revealed that female gender, having an affective disorder, alcohol dependence, higher levels of acculturation stress, and lower levels of education were all predictors of PTSD status. Additionally, alcohol dependence generally occurred after the PTSD diagnosis in early adulthood in this high-risk population. These studies suggest that treatment and prevention efforts should particularly focus on young adult second generation Mexican American women with higher levels of acculturation stress, who may be at higher risk for PTSD, affective disorder, and alcohol dependence following trauma exposure. PMID:27569652

Lifetime history of traumatic events in an American Indian community sample: heritability and relation to substance dependence, affective disorder, conduct disorder and PTSD.

PubMed

Ehlers, Cindy L; Gizer, Ian R; Gilder, David A; Yehuda, Rachael

2013-02-01

American Indians appear to experience a higher rate of traumatic events than what has been reported in general population surveys. American Indians also suffer higher alcohol related death rates than any other ethnic group in the U.S. population. Therefore efforts to delineate factors which may uniquely contribute to increased likelihood of trauma, post traumatic stress disorder (PTSD), and substance use disorders (SUD) over the lifetime in American Indians are important because of the high burden of morbidity and mortality that they pose to American Indian communities. Participants were American Indians recruited from reservations that were assessed with the Semi-Structured Assessment for the Genetics of Alcoholism (SSAGA), family history assessment and the stressful-life-events scale. Of the 309 participants, equivalent numbers of men and women (94%) reported experiencing traumas; however, a larger proportion of women received a PTSD diagnosis (38%) than men (29%). Having experienced multiple trauma and sexual abuse were most highly associated with PTSD. Having experienced assaultive trauma and having PTSD symptoms were both found to be moderately heritable (30-50%). Logistic regression revealed that having an anxiety and/or affective disorder and having a substance dependent diagnosis, but not having antisocial personality disorder/conduct disorder, were significantly correlated with having a diagnosis of PTSD. These studies suggest that trauma is highly prevalent in this American Indian community, it is heritable, is associated with PTSD, affective/anxiety disorders and substance dependence. Additionally, trauma, PTSD and substance dependence appear to all co-emerge in early adulthood in this high-risk population. Copyright © 2012 Elsevier Ltd. All rights reserved.

Lifetime history of traumatic events in a young adult Mexican American sample: Relation to substance dependence, affective disorder, acculturation stress, and PTSD.

PubMed

Ehlers, Cindy L; Kim, Corinne; Gilder, David A; Stouffer, Gina M; Caetano, Raul; Yehuda, Rachel

2016-12-01

Mexican Americans comprise one of the most rapidly growing populations in the United States, and within this population, trauma and post-traumatic stress disorder (PTSD) are associated with physical and mental health problems. Therefore, efforts to delineate factors that may uniquely contribute to increased likelihood of trauma, PTSD, and substance use disorders over the lifetime in Mexican Americans are important to address health disparities and to develop treatment and prevention programs. Six hundred fourteen young adults (age 18-30 yrs) of Mexican American heritage, largely second generation, were recruited from the community and assessed with the Semi-Structured Assessment for the Genetics of Alcoholism and an acculturation stress scale. More males (51.2%) reported experiencing traumas than females (41.1%), however, a larger proportion of females received a PTSD diagnosis (15%) than males (8%). Alcohol dependence and affective disorders, but not anxiety disorders, antisocial disorders, nicotine, marijuana, or stimulant dependence, were significantly comorbid with PTSD. Endorsing higher levels of acculturation stress was also significantly associated with both trauma exposure and a diagnosis of PTSD. Logistic regression revealed that female gender, having an affective disorder, alcohol dependence, higher levels of acculturation stress, and lower levels of education were all predictors of PTSD status. Additionally, alcohol dependence generally occurred after the PTSD diagnosis in early adulthood in this high-risk population. These studies suggest that treatment and prevention efforts should particularly focus on young adult second generation Mexican American women with higher levels of acculturation stress, who may be at higher risk for PTSD, affective disorder, and alcohol dependence following trauma exposure. Copyright © 2016 Elsevier Ltd. All rights reserved.

The effect of 7, 8-methylenedioxylycoctonine-type diterpenoid alkaloids on the toxicity of tall larkspur (Delphinium spp.) in cattle.

PubMed

Welch, K D; Green, B T; Gardner, D R; Cook, D; Pfister, J A; Panter, K E

2012-07-01

Delphinium spp. contain numerous norditerpenoid alkaloids which are structurally delineated as 7, 8-methylenedioxylycoctonine (MDL) and N-(methylsuccinimido) anthranoyllycoctonine (MSAL)-type alkaloids. The toxicity of many tall larkspur species has been primarily attributed to their increased concentration of MSAL-type alkaloids, such as methyllycaconitine (MLA), which are typically 20 times more toxic than MDL-type alkaloids. However, the less toxic MDL-type alkaloids are often more abundant than MSAL-type alkaloids in most Delphinium barbeyi and Delphinium occidentale populations. Previous research demonstrated that MDL-type alkaloids increase the acute toxicity of MSAL-type alkaloids. In this study, we examined the role of MDL-type alkaloids on the overall toxicity of tall larkspur plants to cattle while controlling for the exact dose of MSAL-type alkaloids. Cattle were dosed with plant material from 2 different populations of tall larkspur containing either almost exclusively MDL- or MSAL-type alkaloids. These 2 plant populations were combined to create mixtures with ratios of 0.3:1, 1:1, 5:1, and 10:1 MDL- to MSAL-type alkaloids. The dose that elicited similar clinical signs of poisoning in mice and cattle was determined for each mixture on the basis of the MSAL-type alkaloid content. As the ratio of MDL- to MSAL-type alkaloids increased, the amount of MSAL-type alkaloids required to elicit clinical signs decreased. These results indicate that the less toxic MDL-type alkaloids in tall larkspur exacerbate the toxicity of the MSAL-type alkaloids. Consequently, both the amount of MSAL-type alkaloids and the amount of total alkaloids should be fully characterized to determine more accurately the relative toxicity of tall larkspur plant material.

PGG.Population: a database for understanding the genomic diversity and genetic ancestry of human populations

PubMed Central

Zhang, Chao; Gao, Yang; Liu, Jiaojiao; Xue, Zhe; Lu, Yan; Deng, Lian; Tian, Lei; Feng, Qidi

2018-01-01

Abstract There are a growing number of studies focusing on delineating genetic variations that are associated with complex human traits and diseases due to recent advances in next-generation sequencing technologies. However, identifying and prioritizing disease-associated causal variants relies on understanding the distribution of genetic variations within and among populations. The PGG.Population database documents 7122 genomes representing 356 global populations from 107 countries and provides essential information for researchers to understand human genomic diversity and genetic ancestry. These data and information can facilitate the design of research studies and the interpretation of results of both evolutionary and medical studies involving human populations. The database is carefully maintained and constantly updated when new data are available. We included miscellaneous functions and a user-friendly graphical interface for visualization of genomic diversity, population relationships (genetic affinity), ancestral makeup, footprints of natural selection, and population history etc. Moreover, PGG.Population provides a useful feature for users to analyze data and visualize results in a dynamic style via online illustration. The long-term ambition of the PGG.Population, together with the joint efforts from other researchers who contribute their data to our database, is to create a comprehensive depository of geographic and ethnic variation of human genome, as well as a platform bringing influence on future practitioners of medicine and clinical investigators. PGG.Population is available at https://www.pggpopulation.org. PMID:29112749

Genetic variability and glacial origins of yellow perch (Perca flavescens) in North America

USGS Publications Warehouse

Todd, Thomas N.; Hatcher, Charles O.

1993-01-01

Starch–gel electrophoresis was used to analyze muscle and liver tissue for variation in 13 enzymes representing 31 presumptive loci in yellow perch (Perca flavescens) from 13 localities scattered throughout the natural geographic range of the species in North America. Ten loci were polymorphic, but only three, alcohol dehydrogenase (ADH-1*), glucose-6-phosphate isomerase (GPI-1*), and phosphoglucomutase (PGM-2*), exhibited polymorphisms at relatively high frequencies across localities. Western populations were fixed for one allele at ADH-1*, eastern populations were fixed for another allele, and populations from intermediate locations in Lake Ontario and Pennsylvania had both alleles. The distributions of alleles at GPI-1* and PGM-2*were similar to that of ADH-1*, exhibiting strong differences between eastern and western populations, although the delineation was not as clear. Western populations were much less variable than eastern populations, and the distribution of alleles indicated that the two groups were derived from Mississippi and Atlantic glacial refugia. Populations near the physiographic discontinuity between the Mississippi and Atlantic drainages in western New York and Pennsylvania exhibited an admixture of typically western and eastern alleles. Such observations are consistent with the mixed faunal history of the region and limited postglacial dispersal of western and eastern populations across the boundary.

Polar Bears

USGS Publications Warehouse

Amstrup, Steven C.; Douglas, David C.; Reynolds, Patricia E.; Rhode, E.B.

2002-01-01

Polar bears (Ursus maritimus) are hunted throughout most of their range. In addition to hunting polar bears of the Beaufort Sea region are exposed to mineral and petroleum extraction and related human activities such as shipping road-building, and seismic testing (Stirling 1990).Little was known at the start of this project about how polar bears move about in their environment, and although it was understood that many bears travel across political borders, the boundaries of populations had not been delineated (Amstrup 1986, Amstrup et al. 1986, Amstrup and DeMaster 1988, Garner et al. 1994, Amstrup 1995, Amstrup et al. 1995, Amstrup 2000).As human populations increase and demands for polar bears and other arctic resources escalate, managers must know the sizes and distributions of the polar bear populations. Resource managers also need reliable estimates of breeding rates, reproductive intervals, litter sizes, and survival of young and adults.Our objectives for this research were 1) to determine the seasonal and annual movements of polar bears in the Beaufort Sea, 2) to define the boundaries of the population(s) using this region, 3) to determine the size and status of the Beaufort Sea polar bear population, and 4) to establish reproduction and survival rates (Amstrup 2000).

Developmental timing differences underlie armor loss across threespine stickleback populations.

PubMed

Currey, Mark C; Bassham, Susan; Perry, Stephen; Cresko, William A

2017-11-01

Comparing ontogenetic patterns within a well-described evolutionary context aids in inferring mechanisms of change, including heterochronies or deletion of developmental pathways. Because selection acts on phenotypes throughout ontogeny, any within-taxon developmental variation has implications for evolvability. We compare ontogenetic order and timing of locomotion and defensive traits in three populations of threespine stickleback that have evolutionarily divergent adult forms. This analysis adds to the growing understanding of developmental genetic mechanisms of adaptive change in this evolutionary model species by delineating when chondrogenesis and osteogenesis in two derived populations begin to deviate from the developmental pattern in their immediate ancestors. We found that differences in adult defensive morphologies arise through abolished or delayed initiation of these traits rather than via an overall heterochronic shift, that intra-population ontogenetic variation is increased for some derived traits, and that altered armor developmental timing differentiates the derived populations from each other despite parallels in adult lateral plate armor phenotypes. We found that changes in ossified elements of the pelvic armor are linked to delayed and incomplete development of an early-forming pelvic cartilage, and that this disruption likely presages the variable pelvic vestiges documented in many derived populations. © 2017 Wiley Periodicals, Inc.

Evaluation of the eastern (Centrocercus urophasianus urophasianus) and western (Centrocercus urophasianus phaios) subspecies of Sage-grouse using mitochondrial control-region sequence data

USGS Publications Warehouse

Benedict, N.G.; Oyler-McCance, S.J.; Taylor, S.E.; Braun, C.E.

2003-01-01

The status of Sage-grouse (Centrocercus urophasianus) is of increasing concern, as populations throughout its range have contracted as a result of habitat loss and degradation. Historically, Sage-grouse were classified into two subspecies: eastern(C. u. urophasianus) and western Sage-grouse (C. u. phaios) based on slight differences in coloration noted among eight individuals sampled from Washington, Oregon, and California. We sequenced a rapidly evolving portion of the mitochondrial control region in 332 birds from 16 populations. Although our sampling area covers the proposed boundary between the eastern and western subspecies, no genetic evidence to support the delineation of these subspecies was found. However, a population straddling southwestern Nevada and eastern California was found to contain an unusually high proportion of unique haplotypes, consistent with its genetic isolation from other Sage-grouse populations. Of additional interest was the lack of diversity in the two populations sampled from Washington, one of which contained only a single haplotype. We suggest that multiple lines of evidence are valuable for the formulation of conservation strategies and hence the southwestern Nevada/eastern California population merits further morphological, behavioral, and molecular investigation.

How Useful Is Electroencephalography in the Diagnosis of Autism Spectrum Disorders and the Delineation of Subtypes: A Systematic Review

PubMed Central

Gurau, Oana; Bosl, William J.; Newton, Charles R.

2017-01-01

Autism spectrum disorders (ASD) are thought to be associated with abnormal neural connectivity. Presently, neural connectivity is a theoretical construct that cannot be easily measured. Research in network science and time series analysis suggests that neural network structure, a marker of neural activity, can be measured with electroencephalography (EEG). EEG can be quantified by different methods of analysis to potentially detect brain abnormalities. The aim of this review is to examine evidence for the utility of three methods of EEG signal analysis in the ASD diagnosis and subtype delineation. We conducted a review of literature in which 40 studies were identified and classified according to the principal method of EEG analysis in three categories: functional connectivity analysis, spectral power analysis, and information dynamics. All studies identified significant differences between ASD patients and non-ASD subjects. However, due to high heterogeneity in the results, generalizations could not be inferred and none of the methods alone are currently useful as a new diagnostic tool. The lack of studies prevented the analysis of these methods as tools for ASD subtypes delineation. These results confirm EEG abnormalities in ASD, but as yet not sufficient to help in the diagnosis. Future research with larger samples and more robust study designs could allow for higher sensitivity and consistency in characterizing ASD, paving the way for developing new means of diagnosis. PMID:28747892

A complete-pelvis segmentation framework for image-free total hip arthroplasty (THA): methodology and clinical study.

PubMed

Xie, Weiguo; Franke, Jochen; Chen, Cheng; Grützner, Paul A; Schumann, Steffen; Nolte, Lutz-P; Zheng, Guoyan

2015-06-01

Complete-pelvis segmentation in antero-posterior pelvic radiographs is required to create a patient-specific three-dimensional pelvis model for surgical planning and postoperative assessment in image-free navigation of total hip arthroplasty. A fast and robust framework for accurately segmenting the complete pelvis is presented, consisting of two consecutive modules. In the first module, a three-stage method was developed to delineate the left hemi-pelvis based on statistical appearance and shape models. To handle complex pelvic structures, anatomy-specific information processing techniques were employed. As the input to the second module, the delineated left hemi-pelvis was then reflected about an estimated symmetry line of the radiograph to initialize the right hemi-pelvis segmentation. The right hemi-pelvis was segmented by the same three-stage method, Two experiments conducted on respectively 143 and 40 AP radiographs demonstrated a mean segmentation accuracy of 1.61±0.68 mm. A clinical study to investigate the postoperative assessment of acetabular cup orientations based on the proposed framework revealed an average accuracy of 1.2°±0.9° and 1.6°±1.4° for anteversion and inclination, respectively. Delineation of each radiograph costs less than one minute. Despite further validation needed, the preliminary results implied the underlying clinical applicability of the proposed framework for image-free THA. Copyright © 2014 John Wiley & Sons, Ltd.

On the status of knowledge for using punishment implications for treating behavior disorders.

PubMed Central

Lerman, Dorothea C; Vorndran, Christina M

2002-01-01

In this paper, we review basic and applied findings on punishment and discuss the importance of conducting further research in this area. The characteristics of responding during punishment and numerous factors that interact with basic processes are delineated in conjunction with implications for the treatment of behavior disorders in clinical populations. We conclude that further understanding of punishment processes is needed to develop a highly systematic, effective technology of behavior change, including strategies for improving the efficacy of less intrusive procedures and for successfully fading treatment. PMID:12555918

Driver response to delineation treatments on horizontal curves on two-lane roads.

DOT National Transportation Integrated Search

2009-05-01

The delineation of horizontal curves on two-lane rural roads is an important component of safety : improvements to reduce run-off-road and head-on crashes. This project assessed four types of vertical : delineation in conjunction with edgeline markin...