Microchannel cross load array with dense parallel input

DOEpatents

Swierkowski, Stefan P.

2004-04-06

An architecture or layout for microchannel arrays using T or Cross (+) loading for electrophoresis or other injection and separation chemistry that are performed in microfluidic configurations. This architecture enables a very dense layout of arrays of functionally identical shaped channels and it also solves the problem of simultaneously enabling efficient parallel shapes and biasing of the input wells, waste wells, and bias wells at the input end of the separation columns. One T load architecture uses circular holes with common rows, but not columns, which allows the flow paths for each channel to be identical in shape, using multiple mirror image pieces. Another T load architecture enables the access hole array to be formed on a biaxial, collinear grid suitable for EDM micromachining (square holes), with common rows and columns.

Using Network Theory to Understand Seismic Noise in Dense Arrays

NASA Astrophysics Data System (ADS)

Riahi, N.; Gerstoft, P.

2015-12-01

Dense seismic arrays offer an opportunity to study anthropogenic seismic noise sources with unprecedented detail. Man-made sources typically have high frequency, low intensity, and propagate as surface waves. As a result attenuation restricts their measurable footprint to a small subset of sensors. Medium heterogeneities can further introduce wave front perturbations that limit processing based on travel time. We demonstrate a non-parametric technique that can reliably identify very local events within the array as a function of frequency and time without using travel-times. The approach estimates the non-zero support of the array covariance matrix and then uses network analysis tools to identify clusters of sensors that are sensing a common source. We verify the method on simulated data and then apply it to the Long Beach (CA) geophone array. The method exposes a helicopter traversing the array, oil production facilities with different characteristics, and the fact that noise sources near roads tend to be around 10-20 Hz.

Accessing sparse arrays in parallel memories

DOE Office of Scientific and Technical Information (OSTI.GOV)

Banerjee, U.; Gajski, D.; Kuck, D.

The concept of dense and sparse execution of arrays is introduced. Arrays themselves can be stored in a dense or sparse manner in a parallel memory with m memory modules. The paper proposes hardware for speeding up the execution of array operations of the form c(c/sub 0/+ci)=a(a/sub 0/+ai) op b(b/sub 0/+bi), where a/sub 0/, a, b/sub 0/, b, c/sub 0/, c are integer constants and i is an index variable. The hardware handles 'sparse execution', in which the operation op is not executed for every value of i. The hardware also makes provision for 'sparse storage', in which memory spacemore » is not provided for every array element. It is shown how to access array elements of the above form without conflict in an efficient way. The efficiency is obtained by using some specialised units which are basically smart memories with priority detection, one's counting or associative searching. Generalisation to multidimensional arrays is shown possible under restrictions defined in the paper. 12 references.« less

Retrieving Coherent Receiver Function Images with Dense Arrays

NASA Astrophysics Data System (ADS)

Zhong, M.; Zhan, Z.

2016-12-01

Receiver functions highlight converted phases (e.g., Ps, PpPs, sP) and have been widely used to study seismic interfaces. With a dense array, receiver functions (RFs) at multiple stations form a RF image that can provide more robust/detailed constraints. However, due to noise in data, non-uniqueness of deconvolution, and local structures that cannot be detected across neighboring stations, traditional RF images are often noisy and hard to interpret. Previous attempts to enhance coherence by stacking RFs from multiple events or post-filtering the RF images have not produced satisfactory improvements. Here, we propose a new method to retrieve coherent RF images with dense arrays. We take advantage of the waveform coherency at neighboring stations and invert for a small number of coherent arrivals for their RFs. The new RF images contain only the coherent arrivals required to fit data well. Synthetic tests and preliminary applications on real data demonstrate that the new RF images are easier to interpret and improve our ability to infer Earth structures using receiver functions.

A Generic and Efficient E-field Parallel Imaging Correlator for Next-Generation Radio Telescopes

NASA Astrophysics Data System (ADS)

Thyagarajan, Nithyanandan; Beardsley, Adam P.; Bowman, Judd D.; Morales, Miguel F.

2017-05-01

Modern radio telescopes are favouring densely packed array layouts with large numbers of antennas (NA ≳ 1000). Since the complexity of traditional correlators scales as O(N_A^2), there will be a steep cost for realizing the full imaging potential of these powerful instruments. Through our generic and efficient E-field Parallel Imaging Correlator (epic), we present the first software demonstration of a generalized direct imaging algorithm, namely the Modular Optimal Frequency Fourier imager. Not only does it bring down the cost for dense layouts to O(N_A log _2N_A) but can also image from irregular layouts and heterogeneous arrays of antennas. epic is highly modular, parallelizable, implemented in object-oriented python, and publicly available. We have verified the images produced to be equivalent to those from traditional techniques to within a precision set by gridding coarseness. We have also validated our implementation on data observed with the Long Wavelength Array (LWA1). We provide a detailed framework for imaging with heterogeneous arrays and show that epic robustly estimates the input sky model for such arrays. Antenna layouts with dense filling factors consisting of a large number of antennas such as LWA, the Square Kilometre Array, Hydrogen Epoch of Reionization Array, and Canadian Hydrogen Intensity Mapping Experiment will gain significant computational advantage by deploying an optimized version of epic. The algorithm is a strong candidate for instruments targeting transient searches of fast radio bursts as well as planetary and exoplanetary phenomena due to the availability of high-speed calibrated time-domain images and low output bandwidth relative to visibility-based systems.

Engineering of oriented myocardium on three-dimensional micropatterned collagen-chitosan hydrogel.

PubMed

Chiu, Loraine L Y; Janic, Katarina; Radisic, Milica

2012-04-30

Surface topography and electrical field stimulation are important guidance cues that aid the organization and contractility of cardiomyocytes in vivo. We report here on the use of these biomimetic cues in vitro to engineer an implantable contractile cardiac tissue. Photocrosslinkable collagen-chitosan hydrogels with microgrooves of 10 µm, 20 µm and 100 µm in width were fabricated using polydimethylsiloxane (PDMS) molds. The hydrogels were seeded with cardiomyocytes, placed into a bioreactor array with the microgrooves aligned with the electrical field lines, and stimulated with biphasic square pulses at 1 Hz and 2.5 V/cm. At Day 6, cardiomyocytes were aligned in the direction of the microgrooves. When cultivated without electrical stimulation, the excitation threshold of engineered cardiac tissues using micropatterned hydrogels was significantly lower than using smooth hydrogels, thus showing the importance of cell alignment to cardiac function. The success rate of achieving beating constructs was higher with the application of electrical stimulation. In addition, formation of dense contractile cardiac organoids was observed in groups with both biomimetic cues. The cultivation of cardiomyocytes on hydrogels with 10 µm grooves yielded 100% beating tissues with or without electrical stimulation, thus suggesting a smaller groove width is necessary for cells to communicate and form proper gap junctions. However, electrical field stimulation further increased cell density and enhanced tissue morphology which may be essential for the integration of the tissue construct to the native heart tissue upon implantation. The biodegradability of the hydrogel substrate allows for the rapid translation of the engineered, oriented cardiac tissue to clinical applications.

Magneto-mechanical bone growth stimulation by actuation of highly porous ferromagnetic fiber arrays

NASA Astrophysics Data System (ADS)

Markaki, Athina E.; Clyne, Trevor W.

2005-02-01

This work relates to porous material made by bonding together fibres of a magnetic material. When subjected to a magnetic field, the array deforms, with individual fibres becoming magnetised along their length and then tending to line up locally with the direction of the field. An investigation is presented into the concept that this deformation could induce beneficial strains in bone tissue network in the early stages of growth as it grows into the porous fibre array. An analytical model has been developed, based on the deflection of individual fibre segments (between joints) experiencing bending moments as a result of the induced magnetic dipole. The model has been validated via measurements made on simple fibre assemblies and random fibre arrays. Work has also been done on the deformation characteristics of random fibre arrays with a matrix filling the inter-fibre space. This has the effect of reducing the fibre deflections. The extent of this reduction, and an estimate of the maximum strains induced in the space-filling material, can be obtained using a simple force balance approach. Predictions indicate that in-growing bone tissue, with a stiffness of around 0.01-0.1 GPa, could be strained to beneficial levels (~1 millistrain), using magnetic field strengths in current diagnostic use (~1 Tesla), provided the fibre segment aspect ratio is at least about 10. Such material has a low Young"s modulus, but the overall stiffness of a prosthesis could be matched to that of cortical bone by using an integrated design involving a porous magneto-active layer bonded to a dense non-magnetic core.

High-resolution lithospheric imaging with seismic interferometry

NASA Astrophysics Data System (ADS)

Ruigrok, Elmer; Campman, Xander; Draganov, Deyan; Wapenaar, Kees

2010-10-01

In recent years, there has been an increase in the deployment of relatively dense arrays of seismic stations. The availability of spatially densely sampled global and regional seismic data has stimulated the adoption of industry-style imaging algorithms applied to converted- and scattered-wave energy from distant earthquakes, leading to relatively high-resolution images of the lower crust and upper mantle. We use seismic interferometry to extract reflection responses from the coda of transmitted energy from distant earthquakes. In theory, higher-resolution images can be obtained when migrating reflections obtained with seismic interferometry rather than with conversions, traditionally used in lithospheric imaging methods. Moreover, reflection data allow the straightforward application of algorithms previously developed in exploration seismology. In particular, the availability of reflection data allows us to extract from it a velocity model using standard multichannel data-processing methods. However, the success of our approach relies mainly on a favourable distribution of earthquakes. In this paper, we investigate how the quality of the reflection response obtained with interferometry is influenced by the distribution of earthquakes and the complexity of the transmitted wavefields. Our analysis shows that a reasonable reflection response could be extracted if (1) the array is approximately aligned with an active zone of earthquakes, (2) different phase responses are used to gather adequate angular illumination of the array and (3) the illumination directions are properly accounted for during processing. We illustrate our analysis using a synthetic data set with similar illumination and source-side reverberation characteristics as field data recorded during the 2000-2001 Laramie broad-band experiment. Finally, we apply our method to the Laramie data, retrieving reflection data. We extract a 2-D velocity model from the reflections and use this model to migrate the data. On the final reflectivity image, we observe a discontinuity in the reflections. We interpret this discontinuity as the Cheyenne Belt, a suture zone between Archean and Proterozoic terranes.

ERAASR: an algorithm for removing electrical stimulation artifacts from multielectrode array recordings

NASA Astrophysics Data System (ADS)

O'Shea, Daniel J.; Shenoy, Krishna V.

2018-04-01

Objective. Electrical stimulation is a widely used and effective tool in systems neuroscience, neural prosthetics, and clinical neurostimulation. However, electrical artifacts evoked by stimulation prevent the detection of spiking activity on nearby recording electrodes, which obscures the neural population response evoked by stimulation. We sought to develop a method to clean artifact-corrupted electrode signals recorded on multielectrode arrays in order to recover the underlying neural spiking activity. Approach. We created an algorithm, which performs estimation and removal of array artifacts via sequential principal components regression (ERAASR). This approach leverages the similar structure of artifact transients, but not spiking activity, across simultaneously recorded channels on the array, across pulses within a train, and across trials. The ERAASR algorithm requires no special hardware, imposes no requirements on the shape of the artifact or the multielectrode array geometry, and comprises sequential application of straightforward linear methods with intuitive parameters. The approach should be readily applicable to most datasets where stimulation does not saturate the recording amplifier. Main results. The effectiveness of the algorithm is demonstrated in macaque dorsal premotor cortex using acute linear multielectrode array recordings and single electrode stimulation. Large electrical artifacts appeared on all channels during stimulation. After application of ERAASR, the cleaned signals were quiescent on channels with no spontaneous spiking activity, whereas spontaneously active channels exhibited evoked spikes which closely resembled spontaneously occurring spiking waveforms. Significance. We hope that enabling simultaneous electrical stimulation and multielectrode array recording will help elucidate the causal links between neural activity and cognition and facilitate naturalistic sensory protheses.

Ultrahigh density alignment of carbon nanotube arrays by dielectrophoresis.

PubMed

Shekhar, Shashank; Stokes, Paul; Khondaker, Saiful I

2011-03-22

We report ultrahigh density assembly of aligned single-walled carbon nanotube (SWNT) two-dimensional arrays via AC dielectrophoresis using high-quality surfactant-free and stable SWNT solutions. After optimization of frequency and trapping time, we can reproducibly control the linear density of the SWNT between prefabricated electrodes from 0.5 SWNT/μm to more than 30 SWNT/μm by tuning the concentration of the nanotubes in the solution. Our maximum density of 30 SWNT/μm is the highest for aligned arrays via any solution processing technique reported so far. Further increase of SWNT concentration results in a dense array with multiple layers. We discuss how the orientation and density of the nanotubes vary with concentrations and channel lengths. Electrical measurement data show that the densely packed aligned arrays have low sheet resistances. Selective removal of metallic SWNTs via controlled electrical breakdown produced field-effect transistors with high current on-off ratio. Ultrahigh density alignment reported here will have important implications in fabricating high-quality devices for digital and analog electronics.

Recording and assessment of evoked potentials with electrode arrays.

PubMed

Miljković, N; Malešević, N; Kojić, V; Bijelić, G; Keller, T; Popović, D B

2015-09-01

In order to optimize procedure for the assessment of evoked potentials and to provide visualization of the flow of action potentials along the motor systems, we introduced array electrodes for stimulation and recording and developed software for the analysis of the recordings. The system uses a stimulator connected to an electrode array for the generation of evoked potentials, an electrode array connected to the amplifier, A/D converter and computer for the recording of evoked potentials, and a dedicated software application. The method has been tested for the assessment of the H-reflex on the triceps surae muscle in six healthy humans. The electrode array with 16 pads was positioned over the posterior aspect of the thigh, while the recording electrode array with 16 pads was positioned over the triceps surae muscle. The stimulator activated all the pads of the stimulation electrode array asynchronously, while the signals were recorded continuously at all the recording sites. The results are topography maps (spatial distribution of evoked potentials) and matrices (spatial visualization of nerve excitability). The software allows the automatic selection of the lowest stimulation intensity to achieve maximal H-reflex amplitude and selection of the recording/stimulation pads according to predefined criteria. The analysis of results shows that the method provides rich information compared with the conventional recording of the H-reflex with regard the spatial distribution.

Evaluation of a dense seismic array for acquisition of high quality data in the ACROSS observation

NASA Astrophysics Data System (ADS)

Tsuruga, K.; Kunitomo, T.; Hasada, Y.; Kumazawa, M.; Shigeta, N.; Kasahara, J.

2004-12-01

ACROSS is an active monitoring methodology to detect any subtle temporal change of physical properties in the Earth's interior. We demonstrate the potentiality of the ACROSS observation with a dense sensor array. We have conducted a dense seismic array observation at the distance of 1 km from the ACROSS source since 2003. The array is composed of 36 three-component velocity seismometers buried at 1.8 m deep in an area 25 m square. All the data are recorded accurately referring to a GPS clock. We derived and analyzed a transfer function (TF) from the source to a receiver by the following steps: (1) evaluating a force vector as source characteristics, (2) converting the observed data to the displacement vectors by incorporating all the corrections of the instruments, (3) stacking the observed data for an enough time to suppress the temporal noise, (4) extracting ACROSS signal and evaluating noise level, (5) representing TF in a tensor form with the estimated errors, (6) slant-stacking with variable ray parameters, (7) estimating the travel times and amplitudes of the wave arrivals by Sompi Event Analysis (Hasada et al., 2001) and representing the result by a pulse sequence, and (8) deriving the polarization vector for each arrival to identify all the wave modes. We analyzed TF of SH-wave component from 16 to 20 Hz as an example. We obtained good quality TF with S/N ratio up to 104 by stacking for 12 days at the step (3). The spatial noise originated from the local heterogeneity around the array was eliminated by the step (6). Several arrivals were recognized within the time windows from 0.6 to 1.8 s. The maximum amplitude of event traces was detected at the travel time of 1.064 s with a ray parameter of 7.9x10-4 s/m. We also found the scattered waves probably generated by the heterogeneities around the array. The ACROSS dense array observation would provide a lot of information on the underground heterogeneities. Consequently, we have the important and challenging subjects: (1) optimum designing of ACROSS array to acquire the better data and (2) development of new theoretical method to deal with the variable types of the wave.

A novel hydrogel electrolyte extender for rapid application of EEG sensors and extended recordings.

PubMed

Kleffner-Canucci, Killian; Luu, Phan; Naleway, John; Tucker, Don M

2012-04-30

Dense-array EEG recordings are now commonplace in research and gaining acceptance in clinical settings. Application of many sensors with traditional electrolytes is time consuming. Saline electrolytes can be used to minimize application time but recording duration is limited due to evaporation. In the present study, we evaluate a NIPAm (N-isopropyl acrylamide:acrylic acid) base electrolyte extender for use with saline electrolytes. Sensor-scalp impedances and EEG data quality acquired with the electrolyte extender are compared with those obtained for saline and an EEG electrolyte commonly used in clinical exams (Elefix). The results show that when used in conjunction with saline, electrode-scalp impedances and data across the EEG spectrum are comparable with those obtained using Elefix EEG paste. When used in conjunction with saline, the electrolyte extender permits rapid application of dense-sensor arrays and stable, high-quality EEG data to be obtained for at least 4.5 h. This is an enabling technology that will make benefits of dense-array EEG recordings practical for clinical applications. Copyright © 2011 Elsevier B.V. All rights reserved.

Fiber-array based optogenetic prosthetic system for stimulation therapy

NASA Astrophysics Data System (ADS)

Gu, Ling; Cote, Chris; Tejeda, Hector; Mohanty, Samarendra

2012-02-01

Recent advent of optogenetics has enabled activation of genetically-targeted neuronal cells using low intensity blue light with high temporal precision. Since blue light is attenuated rapidly due to scattering and absorption in neural tissue, optogenetic treatment of neurological disorders may require stimulation of specific cell types in multiple regions of the brain. Further, restoration of certain neural functions (vision, and auditory etc) requires accurate spatio-temporal stimulation patterns rather than just precise temporal stimulation. In order to activate multiple regions of the central nervous system in 3D, here, we report development of an optogenetic prosthetic comprising of array of fibers coupled to independently-controllable LEDs. This design avoids direct contact of LEDs with the brain tissue and thus does not require electrical and heat isolation, which can non-specifically stimulate and damage the local brain regions. The intensity, frequency, and duty cycle of light pulses from each fiber in the array was controlled independently using an inhouse developed LabView based program interfaced with a microcontroller driving the individual LEDs. While the temporal profile of the light pulses was controlled by varying the current driving the LED, the beam profile emanating from each fiber tip could be sculpted by microfabrication of the fiber tip. The fiber array was used to stimulate neurons, expressing channelrhodopsin-2, in different locations within the brain or retina. Control of neural activity in the mice cortex, using the fiber-array based prosthetic, is evaluated from recordings made with multi-electrode array (MEA). We also report construction of a μLED array based prosthetic for spatio-temporal stimulation of cortex.

Biasing, acquisition, and interpretation of a dense Langmuir probe array in NSTX.

PubMed

Jaworski, M A; Kallman, J; Kaita, R; Kugel, H; LeBlanc, B; Marsala, R; Ruzic, D N

2010-10-01

A dense array of 99 Langmuir probes has been installed in the lower divertor region of the National Spherical Torus Experiment (NSTX). This array is instrumented with a system of electronics that allows flexibility in the choice of probes to bias as well as the type of measurement (including standard swept, single probe, triple probe, and operation as passive floating potential and scrape-off-layer SOL current monitors). The use of flush-mounted probes requires careful interpretation. The time dependent nature of the SOL makes swept-probe traces difficult to interpret. To overcome these challenges, the single- and triple-Langmuir probe signals are used in complementary fashion to determine the temperature and density at the probe location. A comparison to midplane measurements is made.

Optical Pumping of High Power Lasers with an Array of Plasma Pinches.

DTIC Science & Technology

1986-04-01

Two dense plasma focus systems, the hypocycloidal pinch and the Mather type were investigated as the potential excitation light sources for high...was also performed for the first time using the Mather type dense plasma focus (MDPF) sucsessfully. Results thus fare indicate that both HCP and MDPF

Time-jittered marine seismic data acquisition via compressed sensing and sparsity-promoting wavefield reconstruction

NASA Astrophysics Data System (ADS)

Wason, H.; Herrmann, F. J.; Kumar, R.

2016-12-01

Current efforts towards dense shot (or receiver) sampling and full azimuthal coverage to produce high resolution images have led to the deployment of multiple source vessels (or streamers) across marine survey areas. Densely sampled marine seismic data acquisition, however, is expensive, and hence necessitates the adoption of sampling schemes that save acquisition costs and time. Compressed sensing is a sampling paradigm that aims to reconstruct a signal--that is sparse or compressible in some transform domain--from relatively fewer measurements than required by the Nyquist sampling criteria. Leveraging ideas from the field of compressed sensing, we show how marine seismic acquisition can be setup as a compressed sensing problem. A step ahead from multi-source seismic acquisition is simultaneous source acquisition--an emerging technology that is stimulating both geophysical research and commercial efforts--where multiple source arrays/vessels fire shots simultaneously resulting in better coverage in marine surveys. Following the design principles of compressed sensing, we propose a pragmatic simultaneous time-jittered time-compressed marine acquisition scheme where single or multiple source vessels sail across an ocean-bottom array firing airguns at jittered times and source locations, resulting in better spatial sampling and speedup acquisition. Our acquisition is low cost since our measurements are subsampled. Simultaneous source acquisition generates data with overlapping shot records, which need to be separated for further processing. We can significantly impact the reconstruction quality of conventional seismic data from jittered data and demonstrate successful recovery by sparsity promotion. In contrast to random (sub)sampling, acquisition via jittered (sub)sampling helps in controlling the maximum gap size, which is a practical requirement of wavefield reconstruction with localized sparsifying transforms. We illustrate our results with simulations of simultaneous time-jittered marine acquisition for 2D and 3D ocean-bottom cable survey.

Preterm EEG: a multimodal neurophysiological protocol.

PubMed

Stjerna, Susanna; Voipio, Juha; Metsäranta, Marjo; Kaila, Kai; Vanhatalo, Sampsa

2012-02-18

Since its introduction in early 1950s, electroencephalography (EEG) has been widely used in the neonatal intensive care units (NICU) for assessment and monitoring of brain function in preterm and term babies. Most common indications are the diagnosis of epileptic seizures, assessment of brain maturity, and recovery from hypoxic-ischemic events. EEG recording techniques and the understanding of neonatal EEG signals have dramatically improved, but these advances have been slow to penetrate through the clinical traditions. The aim of this presentation is to bring theory and practice of advanced EEG recording available for neonatal units. In the theoretical part, we will present animations to illustrate how a preterm brain gives rise to spontaneous and evoked EEG activities, both of which are unique to this developmental phase, as well as crucial for a proper brain maturation. Recent animal work has shown that the structural brain development is clearly reflected in early EEG activity. Most important structures in this regard are the growing long range connections and the transient cortical structure, subplate. Sensory stimuli in a preterm baby will generate responses that are seen at a single trial level, and they have underpinnings in the subplate-cortex interaction. This brings neonatal EEG readily into a multimodal study, where EEG is not only recording cortical function, but it also tests subplate function via different sensory modalities. Finally, introduction of clinically suitable dense array EEG caps, as well as amplifiers capable of recording low frequencies, have disclosed multitude of brain activities that have as yet been overlooked. In the practical part of this video, we show how a multimodal, dense array EEG study is performed in neonatal intensive care unit from a preterm baby in the incubator. The video demonstrates preparation of the baby and incubator, application of the EEG cap, and performance of the sensory stimulations.

Optoelectronic Integrated Circuits For Neural Networks

NASA Technical Reports Server (NTRS)

Psaltis, D.; Katz, J.; Kim, Jae-Hoon; Lin, S. H.; Nouhi, A.

1990-01-01

Many threshold devices placed on single substrate. Integrated circuits containing optoelectronic threshold elements developed for use as planar arrays of artificial neurons in research on neural-network computers. Mounted with volume holograms recorded in photorefractive crystals serving as dense arrays of variable interconnections between neurons.

Development of a multi-electrode array for spinal cord epidural stimulation to facilitate stepping and standing after a complete spinal cord injury in adult rats.

PubMed

Gad, Parag; Choe, Jaehoon; Nandra, Mandheerej Singh; Zhong, Hui; Roy, Roland R; Tai, Yu-Chong; Edgerton, V Reggie

2013-01-21

Stimulation of the spinal cord has been shown to have great potential for improving function after motor deficits caused by injury or pathological conditions. Using a wide range of animal models, many studies have shown that stimulation applied to the neural networks intrinsic to the spinal cord can result in a dramatic improvement of motor ability, even allowing an animal to step and stand after a complete spinal cord transection. Clinical use of this technology, however, has been slow to develop due to the invasive nature of the implantation procedures, the lack of versatility in conventional stimulation technology, and the difficulty of ascertaining specific sites of stimulation that would provide optimal amelioration of the motor deficits. Moreover, the development of tools available to control precise stimulation chronically via biocompatible electrodes has been limited. In this paper, we outline the development of this technology and its use in the spinal rat model, demonstrating the ability to identify and stimulate specific sites of the spinal cord to produce discrete motor behaviors in spinal rats using this array. We have designed a chronically implantable, rapidly switchable, high-density platinum based multi-electrode array that can be used to stimulate at 1-100 Hz and 1-10 V in both monopolar and bipolar configurations to examine the electrophysiological and behavioral effects of spinal cord epidural stimulation in complete spinal cord transected rats. In this paper, we have demonstrated the effectiveness of using high-resolution stimulation parameters in the context of improving motor recovery after a spinal cord injury. We observed that rats whose hindlimbs were paralyzed can stand and step when specific sets of electrodes of the array are stimulated tonically (40 Hz). Distinct patterns of stepping and standing were produced by stimulation of different combinations of electrodes on the array located at specific spinal cord levels and by specific stimulation parameters, i.e., stimulation frequency and intensity, and cathode/anode orientation. The array also was used to assess functional connectivity between the cord dorsum to interneuronal circuits and specific motor pools via evoked potentials induced at 1 Hz stimulation in the absence of any anesthesia. Therefore the high density electrode array allows high spatial resolution and the ability to selectively activate different neural pathways within the lumbosacral region of the spinal cord to facilitate standing and stepping in adult spinal rats and provides the capability to evoke motor potentials and thus a means for assessing connectivity between sensory circuits and specific motor pools and muscles.

Comparative efficiency analysis of fiber-array and conventional beam director systems in volume turbulence.

PubMed

Vorontsov, Mikhail; Filimonov, Grigory; Ovchinnikov, Vladimir; Polnau, Ernst; Lachinova, Svetlana; Weyrauch, Thomas; Mangano, Joseph

2016-05-20

The performance of two prominent laser beam projection system types is analyzed through wave-optics numerical simulations for various atmospheric turbulence conditions, propagation distances, and adaptive optics (AO) mitigation techniques. Comparisons are made between different configurations of both a conventional beam director (BD) using a monolithic-optics-based Cassegrain telescope and a fiber-array BD that uses an array of densely packed fiber collimators. The BD systems considered have equal input power and aperture diameters. The projected laser beam power inside the Airy size disk at the target plane is used as the performance metric. For the fiber-array system, both incoherent and coherent beam combining regimes are considered. We also present preliminary results of side-by-side atmospheric beam projection experiments over a 7-km propagation path using both the AO-enhanced beam projection system with a Cassegrain telescope and the coherent fiber-array BD composed of 21 densely packed fiber collimators. Both wave-optics numerical simulation and experimental results demonstrate that, for similar system architectures and turbulence conditions, coherent fiber-array systems are more efficient in mitigation of atmospheric turbulence effects and generation of a hit spot of the smallest possible size on a remotely located target.

Self-assembled nanoparticle arrays as nanomasks for pattern transfer

NASA Astrophysics Data System (ADS)

Sachan, M.; Bonnoit, C.; Hogg, C.; Evarts, E.; Bain, J. A.; Majetich, S. A.; Park, J.-H.; Zhu, J.-G.

2008-07-01

Argon ion milling was used to transfer the pattern of sparse 12 nm iron oxide nanoparticles into underlying thin films of Pt and magnetic tunnel junction stacks and quantify their etching rates and morphological evolution. Under typical milling conditions, Pt milled at 10 nm min-1, while the isolated particles of iron oxide used for the mask milled at 5 nm min-1. Dilute dispersions of nanoparticles were used to produce the sparse nanomasks, and high resolution scanning electron microscopy (SEM) and atomic force microscopy were used to monitor the evolution of etched structures as a function of milling time. SEM measurements indicate an apparent 20% increase in feature diameter before the features began to diminish under additional milling, suggesting redeposition as a limiting feature in the milling of dense arrays. Simulations of the milling process in nanoparticle arrays that include redeposition are consistent with this observation. These simulations predict that an edge-to-edge spacing of 3 nm in a dense array is feasible, but that redeposition reduces the final structure aspect ratio from that of the masking array by as much as a factor of two.

A Plasma Ultraviolet Source for Short Wavelength Lasers.

DTIC Science & Technology

1986-03-10

A high power blue-green laser was pumped with an array of the dense plasma focus . As the result of optimizing the operating conditions of the dense... plasma focus and laser system, the maximum untuned laser output exceeded 2.lmJ corresponding to the energy density 3J/cu cm which is much higher than

Interdigitated array of Pt electrodes for electrical stimulation and engineering of aligned muscle tissue.

PubMed

Ahadian, Samad; Ramón-Azcón, Javier; Ostrovidov, Serge; Camci-Unal, Gulden; Hosseini, Vahid; Kaji, Hirokazu; Ino, Kosuke; Shiku, Hitoshi; Khademhosseini, Ali; Matsue, Tomokazu

2012-09-21

Engineered skeletal muscle tissues could be useful for applications in tissue engineering, drug screening, and bio-robotics. It is well-known that skeletal muscle cells are able to differentiate under electrical stimulation (ES), with an increase in myosin production, along with the formation of myofibers and contractile proteins. In this study, we describe the use of an interdigitated array of electrodes as a novel platform to electrically stimulate engineered muscle tissues. The resulting muscle myofibers were analyzed and quantified in terms of their myotube characteristics and gene expression. The engineered muscle tissues stimulated through the interdigitated array of electrodes demonstrated superior performance and maturation compared to the corresponding tissues stimulated through a conventional setup (i.e., through Pt wires in close proximity to the muscle tissue). In particular, the ES of muscle tissue (voltage 6 V, frequency 1 Hz and duration 10 ms for 1 day) through the interdigitated array of electrodes resulted in a higher degree of C2C12 myotube alignment (∼80%) as compared to ES using Pt wires (∼65%). In addition, higher amounts of C2C12 myotube coverage area, myotube length, muscle transcription factors and protein biomarkers were found for myotubes stimulated through the interdigitated array of electrodes compared to those stimulated using the Pt wires. Due to the wide array of potential applications of ES for two- and three-dimensional (2D and 3D) engineered tissues, the suggested platform could be employed for a variety of cell and tissue structures to more efficiently investigate their response to electrical fields.

Flexible Neural Electrode Array Based-on Porous Graphene for Cortical Microstimulation and Sensing

NASA Astrophysics Data System (ADS)

Lu, Yichen; Lyu, Hongming; Richardson, Andrew G.; Lucas, Timothy H.; Kuzum, Duygu

2016-09-01

Neural sensing and stimulation have been the backbone of neuroscience research, brain-machine interfaces and clinical neuromodulation therapies for decades. To-date, most of the neural stimulation systems have relied on sharp metal microelectrodes with poor electrochemical properties that induce extensive damage to the tissue and significantly degrade the long-term stability of implantable systems. Here, we demonstrate a flexible cortical microelectrode array based on porous graphene, which is capable of efficient electrophysiological sensing and stimulation from the brain surface, without penetrating into the tissue. Porous graphene electrodes show superior impedance and charge injection characteristics making them ideal for high efficiency cortical sensing and stimulation. They exhibit no physical delamination or degradation even after 1 million biphasic stimulation cycles, confirming high endurance. In in vivo experiments with rodents, same array is used to sense brain activity patterns with high spatio-temporal resolution and to control leg muscles with high-precision electrical stimulation from the cortical surface. Flexible porous graphene array offers a minimally invasive but high efficiency neuromodulation scheme with potential applications in cortical mapping, brain-computer interfaces, treatment of neurological disorders, where high resolution and simultaneous recording and stimulation of neural activity are crucial.

Focused intracochlear electric stimulation with phased array channels.

PubMed

van den Honert, Chris; Kelsall, David C

2007-06-01

A method is described for producing focused intracochlear electric stimulation using an array of N electrodes. For each electrode site, N weights are computed that define the ratios of positive and negative electrode currents required to produce cancellation of the voltage within scala tympani at all of the N-1 other sites. Multiple sites can be stimulated simultaneously by superposition of their respective current vectors. The method allows N independent stimulus waveforms to be delivered to each of the N electrode sites without spatial overlap. Channel interaction from current spread associated with monopolar stimulation is substantially eliminated. The method operates by inverting the spread functions of individual monopoles as measured with the other electrodes. The method was implemented and validated with data from three human subjects implanted with 22-electrode perimodiolar arrays. Results indicate that (1) focusing is realizable with realistic precision; (2) focusing comes at the cost of increased total stimulation current; (3) uncanceled voltages that arise beyond the ends of the array are weak except when stimulating the two end channels; and (4) close perimodiolar positioning of the electrodes may be important for minimizing stimulation current and sensitivity to measurement errors.

Sweetwater, Texas Large N Experiment

NASA Astrophysics Data System (ADS)

Sumy, D. F.; Woodward, R.; Barklage, M.; Hollis, D.; Spriggs, N.; Gridley, J. M.; Parker, T.

2015-12-01

From 7 March to 30 April 2014, NodalSeismic, Nanometrics, and IRIS PASSCAL conducted a collaborative, spatially-dense seismic survey with several thousand nodal short-period geophones complemented by a backbone array of broadband sensors near Sweetwater, Texas. This pilot project demonstrates the efficacy of industry and academic partnerships, and leveraged a larger, commercial 3D survey to collect passive source seismic recordings to image the subsurface. This innovative deployment of a large-N mixed-mode array allows industry to explore array geometries and investigate the value of broadband recordings, while affording academics a dense wavefield imaging capability and an operational model for high volume instrument deployment. The broadband array consists of 25 continuously-recording stations from IRIS PASSCAL and Nanometrics, with an array design that maximized recording of horizontal-traveling seismic energy for surface wave analysis over the primary target area with sufficient offset for imaging objectives at depth. In addition, 2639 FairfieldNodal Zland nodes from NodalSeismic were deployed in three sub-arrays: the outlier, backbone, and active source arrays. The backbone array consisted of 292 nodes that covered the entire survey area, while the outlier array consisted of 25 continuously-recording nodes distributed at a ~3 km distance away from the survey perimeter. Both the backbone and outlier array provide valuable constraints for the passive source portion of the analysis. This project serves as a learning platform to develop best practices in the support of large-N arrays with joint industry and academic expertise. Here we investigate lessons learned from a facility perspective, and present examples of data from the various sensors and array geometries. We will explore first-order results from local and teleseismic earthquakes, and show visualizations of the data across the array. Data are archived at the IRIS DMC under stations codes XB and 1B.

Large-eddy simulation of dense gas dispersion over a simplified urban area

NASA Astrophysics Data System (ADS)

Wingstedt, E. M. M.; Osnes, A. N.; Åkervik, E.; Eriksson, D.; Reif, B. A. Pettersson

2017-03-01

Dispersion of neutral and dense gas over a simplified urban area, comprising four cubes, has been investigated by the means of large-eddy simulations (LES). The results have been compared to wind tunnel experiments and both mean and fluctuating quantities of velocity and concentration are in very good agreement. High-quality inflow profiles are necessary to achieve physically realistic LES results. In this study, profiles matching the atmospheric boundary layer flow in the wind tunnel, are generated by means of a separate precursor simulation. Emission of dense gas dramatically alters the flow in the near source region and introduces an upstream dispersion. The resulting dispersion patterns of neutral and dense gas differ significantly, where the plume in the latter case is wider and shallower. The dense gas is highly affected by the cube array, which seems to act as a barrier, effectively deflecting the plume. This leads to higher concentrations outside of the array than inside. On the contrary, the neutral gas plume has a Gaussian-type shape, with highest concentrations along the centreline. It is found that the dense gas reduces the vertical and spanwise turbulent momentum transport and, as a consequence, the turbulence kinetic energy. The reduction coincides with the area where the gradient Richardson number exceeds its critical value, i.e. where the flow may be characterized as stably stratified. Interestingly, this region does not correspond to where the concentration of dense gas is the highest (close to the ground), as this is also where the largest velocity gradients are to be found. Instead there is a layer in the middle of the dense gas cloud where buoyancy is dynamically dominant.

Nanowire array and nanowire solar cells and methods for forming the same

DOEpatents

Yang, Peidong [Berkeley, CA; Greene, Lori [Berkeley, CA; Law, Matthew [Berkeley, CA

2007-09-04

Homogeneous and dense arrays of nanowires are described. The nanowires can be formed in solution and can have average diameters of 40-300 nm and lengths of 1-3 .mu.m. They can be formed on any suitable substrate. Photovoltaic devices are also described.

Nanowire array and nanowire solar cells and methods for forming the same

DOEpatents

Yang, Peidong; Greene, Lori E.; Law, Matthew

2009-06-09

Homogeneous and dense arrays of nanowires are described. The nanowires can be formed in solution and can have average diameters of 40-300 nm and lengths of 1-3 .mu.m. They can be formed on any suitable substrate. Photovoltaic devices are also described.

M&A For Lithography Of Sparse Arrays Of Sub-Micrometer Features

DOEpatents

Brueck, Steven R.J.; Chen, Xiaolan; Zaidi, Saleem; Devine, Daniel J.

1998-06-02

Methods and apparatuses are disclosed for the exposure of sparse hole and/or mesa arrays with line:space ratios of 1:3 or greater and sub-micrometer hole and/or mesa diameters in a layer of photosensitive material atop a layered material. Methods disclosed include: double exposure interferometric lithography pairs in which only those areas near the overlapping maxima of each single-period exposure pair receive a clearing exposure dose; double interferometric lithography exposure pairs with additional processing steps to transfer the array from a first single-period interferometric lithography exposure pair into an intermediate mask layer and a second single-period interferometric lithography exposure to further select a subset of the first array of holes; a double exposure of a single period interferometric lithography exposure pair to define a dense array of sub-micrometer holes and an optical lithography exposure in which only those holes near maxima of both exposures receive a clearing exposure dose; combination of a single-period interferometric exposure pair, processing to transfer resulting dense array of sub-micrometer holes into an intermediate etch mask, and an optical lithography exposure to select a subset of initial array to form a sparse array; combination of an optical exposure, transfer of exposure pattern into an intermediate mask layer, and a single-period interferometric lithography exposure pair; three-beam interferometric exposure pairs to form sparse arrays of sub-micrometer holes; five- and four-beam interferometric exposures to form a sparse array of sub-micrometer holes in a single exposure. Apparatuses disclosed include arrangements for the three-beam, five-beam and four-beam interferometric exposures.

Vertically aligned carbon nanotubes for microelectrode arrays applications.

PubMed

Castro Smirnov, J R; Jover, Eric; Amade, Roger; Gabriel, Gemma; Villa, Rosa; Bertran, Enric

2012-09-01

In this work a methodology to fabricate carbon nanotube based electrodes using plasma enhanced chemical vapour deposition has been explored and defined. The final integrated microelectrode based devices should present specific properties that make them suitable for microelectrode arrays applications. The methodology studied has been focused on the preparation of highly regular and dense vertically aligned carbon nanotube (VACNT) mat compatible with the standard lithography used for microelectrode arrays technology.

Biasing, Acquisition and Interpretation of a Dense Langmuir Probe Array in NSTX

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jaworski, M. A.; Kallman, J.; Kaita, R.

2010-09-22

A dense array of 99 Langmuir probes has been installed in the lower divertor region of the National Spherical Torus Experiments (NSTX). This array is instrumented with a system of elec- tronics that allows flexibility in the choice of probes to bias as well as the type of measurement (including standard swept, single probe, triple probe and operation as passive floating potential and scrape-off-layer (SOL) current monitors). The use of flush-mounted probes requires careful inter- pretation. The time dependent nature of the SOL makes swept-probe traces difficult to interpret. To overcome these challenges, the single- and triple-Langmuir probe signals aremore » used in comple- mentary fashion to determine the temperature and density at the probe location. A comparison to mid-plane measurements is made. Work is supported by DOE contracts DE-AC02-09CHI1466 and DE-PS02-07ER07-29.« less

Dense water plumes modulate richness and productivity of deep sea microbes.

PubMed

Luna, Gian Marco; Chiggiato, Jacopo; Quero, Grazia Marina; Schroeder, Katrin; Bongiorni, Lucia; Kalenitchenko, Dimitri; Galand, Pierre E

2016-12-01

Growing evidence indicates that dense water formation and flow over the continental shelf is a globally relevant oceanographic process, potentially affecting microbial assemblages down to the deep ocean. However, the extent and consequences of this influence have yet to be investigated. Here it is shown that dense water propagation to the deep ocean increases the abundance of prokaryotic plankton, and stimulates carbon production and organic matter degradation rates. Dense waters spilling off the shelf modifies community composition of deep sea microbial assemblages, leading to the increased relevance of taxa likely originating from the sea surface and the seafloor. This phenomenon can be explained by a combination of factors that interplay during the dense waters propagation, such as the transport of surface microbes to the ocean floor (delivering in our site 0.1 megatons of C), the stimulation of microbial metabolism due to increased ventilation and nutrients availability, the sediment re-suspension, and the mixing with ambient waters along the path. Thus, these results highlight a hitherto unidentified role for dense currents flowing over continental shelves in influencing deep sea microbes. In light of climate projections, this process will affect significantly the microbial functioning and biogeochemical cycling of large sectors of the ocean interior. © 2016 Society for Applied Microbiology and John Wiley & Sons Ltd.

Teleseismic array analysis of upper mantle compressional velocity structure. Ph.D. Thesis

NASA Technical Reports Server (NTRS)

Walck, M. C.

1984-01-01

Relative array analysis of upper mantle lateral velocity variations in southern California, analysis techniques for dense data profiles, the P-wave upper mantle structure beneath an active spreading center: the Gulf of California, and the upper mantle under the Cascade ranges: a comparison with the Gulf of California are presented.

Charge injection and discharging of Si nanocrystals and arrays by atomic force microscopy

NASA Technical Reports Server (NTRS)

Boer, E.; Ostraat, M.; Brongersma, M. L.; Flagan, R. C.; Atwater, H. A.

2000-01-01

Charge injection and storage in dense arrays of silicon nanocrystals in SiO(sub 2) is a critical aspect of the performance of potential nanocrystal flash memory structures. The ultimate goal for this class of devices is few-or single- electron storage in a small number of nanocrystal elements.

High-Throughput Fabrication of Ultradense Annular Nanogap Arrays for Plasmon-Enhanced Spectroscopy.

PubMed

Cai, Hongbing; Meng, Qiushi; Zhao, Hui; Li, Mingling; Dai, Yanmeng; Lin, Yue; Ding, Huaiyi; Pan, Nan; Tian, Yangchao; Luo, Yi; Wang, Xiaoping

2018-06-13

The confinement of light into nanometer-sized metallic nanogaps can lead to an extremely high field enhancement, resulting in dramatically enhanced absorption, emission, and surface-enhanced Raman scattering (SERS) of molecules embedded in nanogaps. However, low-cost, high-throughput, and reliable fabrication of ultra-high-dense nanogap arrays with precise control of the gap size still remains a challenge. Here, by combining colloidal lithography and atomic layer deposition technique, a reproducible method for fabricating ultra-high-dense arrays of hexagonal close-packed annular nanogaps over large areas is demonstrated. The annular nanogap arrays with a minimum diameter smaller than 100 nm and sub-1 nm gap width have been produced, showing excellent SERS performance with a typical enhancement factor up to 3.1 × 10 6 and a detection limit of 10 -11 M. Moreover, it can also work as a high-quality field enhancement substrate for studying two-dimensional materials, such as MoSe 2 . Our method provides an attractive approach to produce controllable nanogaps for enhanced light-matter interaction at the nanoscale.

A New Semi-Automatic Approach to Find Suitable Virtual Electrodes in Arrays Using an Interpolation Strategy.

PubMed

Salchow, Christina; Valtin, Markus; Seel, Thomas; Schauer, Thomas

2016-06-13

Functional Electrical Stimulation via electrode arrays enables the user to form virtual electrodes (VEs) of dynamic shape, size, and position. We developed a feedback-control-assisted manual search strategy which allows the therapist to conveniently and continuously modify VEs to find a good stimulation area. This works for applications in which the desired movement consists of at least two degrees of freedom. The virtual electrode can be moved to arbitrary locations within the array, and each involved element is stimulated with an individual intensity. Meanwhile, the applied global stimulation intensity is controlled automatically to meet a predefined angle for one degree of freedom. This enables the therapist to concentrate on the remaining degree(s) of freedom while changing the VE position. This feedback-control-assisted approach aims to integrate the user's opinion and the patient's sensation. Therefore, our method bridges the gap between manual search and fully automatic identification procedures for array electrodes. Measurements in four healthy volunteers were performed to demonstrate the usefulness of our concept, using a 24-element array to generate wrist and hand extension.

Reducing interaction in simultaneous paired stimulation with CI.

PubMed

Vellinga, Dirk; Bruijn, Saskia; Briaire, Jeroen J; Kalkman, Randy K; Frijns, Johan H M

2017-01-01

In this study simultaneous paired stimulation of electrodes in cochlear implants is investigated by psychophysical experiments in 8 post-lingually deaf subjects (and one extra subject who only participated in part of the experiments). Simultaneous and sequential monopolar stimulation modes are used as references and are compared to channel interaction compensation, partial tripolar stimulation and a novel sequential stimulation strategy named phased array compensation. Psychophysical experiments are performed to investigate both the loudness integration during paired stimulation at the main electrodes as well as the interaction with the electrode contact located halfway between the stimulating pair. The study shows that simultaneous monopolar stimulation has more loudness integration on the main electrodes and more interaction in between the electrodes than sequential stimulation. Channel interaction compensation works to reduce the loudness integration at the main electrodes, but does not reduce the interaction in between the electrodes caused by paired stimulation. Partial tripolar stimulation uses much more current to reach the needed loudness, but shows the same interaction in between the electrodes as sequential monopolar stimulation. In phased array compensation we have used the individual impedance matrix of each subject to calculate the current needed on each electrode to exactly match the stimulation voltage along the array to that of sequential stimulation. The results show that the interaction in between the electrodes is the same as monopolar stimulation. The strategy uses less current than partial tripolar stimulation, but more than monopolar stimulation. In conclusion, the paper shows that paired stimulation is possible if the interaction is compensated.

RhoG protein regulates platelet granule secretion and thrombus formation in mice.

PubMed

Goggs, Robert; Harper, Matthew T; Pope, Robert J; Savage, Joshua S; Williams, Christopher M; Mundell, Stuart J; Heesom, Kate J; Bass, Mark; Mellor, Harry; Poole, Alastair W

2013-11-22

Rho GTPases such as Rac, RhoA, and Cdc42 are vital for normal platelet function, but the role of RhoG in platelets has not been studied. In other cells, RhoG orchestrates processes integral to platelet function, including actin cytoskeletal rearrangement and membrane trafficking. We therefore hypothesized that RhoG would play a critical role in platelets. Here, we show that RhoG is expressed in human and mouse platelets and is activated by both collagen-related peptide (CRP) and thrombin stimulation. We used RhoG(-/-) mice to study the function of RhoG in platelets. Integrin activation and aggregation were reduced in RhoG(-/-) platelets stimulated by CRP, but responses to thrombin were normal. The central defect in RhoG(-/-) platelets was reduced secretion from α-granules, dense granules, and lysosomes following CRP stimulation. The integrin activation and aggregation defects could be rescued by ADP co-stimulation, indicating that they are a consequence of diminished dense granule secretion. Defective dense granule secretion in RhoG(-/-) platelets limited recruitment of additional platelets to growing thrombi in flowing blood in vitro and translated into reduced thrombus formation in vivo. Interestingly, tail bleeding times were normal in RhoG(-/-) mice, suggesting that the functions of RhoG in platelets are particularly relevant to thrombotic disorders.

Single-cell recording and stimulation with a 16k micro-nail electrode array integrated on a 0.18 μm CMOS chip.

PubMed

Huys, Roeland; Braeken, Dries; Jans, Danny; Stassen, Andim; Collaert, Nadine; Wouters, Jan; Loo, Josine; Severi, Simone; Vleugels, Frank; Callewaert, Geert; Verstreken, Kris; Bartic, Carmen; Eberle, Wolfgang

2012-04-07

To cope with the growing needs in research towards the understanding of cellular function and network dynamics, advanced micro-electrode arrays (MEAs) based on integrated complementary metal oxide semiconductor (CMOS) circuits have been increasingly reported. Although such arrays contain a large number of sensors for recording and/or stimulation, the size of the electrodes on these chips are often larger than a typical mammalian cell. Therefore, true single-cell recording and stimulation remains challenging. Single-cell resolution can be obtained by decreasing the size of the electrodes, which inherently increases the characteristic impedance and noise. Here, we present an array of 16,384 active sensors monolithically integrated on chip, realized in 0.18 μm CMOS technology for recording and stimulation of individual cells. Successful recording of electrical activity of cardiac cells with the chip, validated with intracellular whole-cell patch clamp recordings are presented, illustrating single-cell readout capability. Further, by applying a single-electrode stimulation protocol, we could pace individual cardiac cells, demonstrating single-cell addressability. This novel electrode array could help pave the way towards solving complex interactions of mammalian cellular networks. This journal is © The Royal Society of Chemistry 2012

Core Emergence in a Massive Infrared Dark Cloud: A Comparison between Mid-IR Extinction and 1.3 mm Emission

NASA Astrophysics Data System (ADS)

Kong, Shuo; Tan, Jonathan C.; Arce, Héctor G.; Caselli, Paola; Fontani, Francesco; Butler, Michael J.

2018-03-01

Stars are born from dense cores in molecular clouds. Observationally, it is crucial to capture the formation of cores in order to understand the necessary conditions and rate of the star formation process. The Atacama Large Millimeter/submillimeter Array (ALMA) is extremely powerful for identifying dense gas structures, including cores, at millimeter wavelengths via their dust continuum emission. Here, we use ALMA to carry out a survey of dense gas and cores in the central region of the massive (∼105 M ⊙) infrared dark cloud (IRDC) G28.37+0.07. The observation consists of a mosaic of 86 pointings of the 12 m array and produces an unprecedented view of the densest structures of this IRDC. In this first Letter about this data set, we focus on a comparison between the 1.3 mm continuum emission and a mid-infrared (MIR) extinction map of the IRDC. This allows estimation of the “dense gas” detection probability function (DPF), i.e., as a function of the local mass surface density, Σ, for various choices of thresholds of millimeter continuum emission to define “dense gas.” We then estimate the dense gas mass fraction, f dg, in the central region of the IRDC and, via extrapolation with the DPF and the known Σ probability distribution function, to the larger-scale surrounding regions, finding values of about 5% to 15% for the fiducial choice of threshold. We argue that this observed dense gas is a good tracer of the protostellar core population and, in this context, estimate a star formation efficiency per free-fall time in the central IRDC region of ɛ ff ∼ 10%, with approximately a factor of two systematic uncertainties.

Estimating seismic site response in Christchurch City (New Zealand) from dense low-cost aftershock arrays

USGS Publications Warehouse

Kaiser, Anna E.; Benites, Rafael A.; Chung, Angela I.; Haines, A. John; Cochran, Elizabeth S.; Fry, Bill

2011-01-01

The Mw 7.1 September 2010 Darfield earthquake, New Zealand, produced widespread damage and liquefaction ~40 km from the epicentre in Christchurch city. It was followed by the even more destructive Mw 6.2 February 2011 Christchurch aftershock directly beneath the city’s southern suburbs. Seismic data recorded during the two large events suggest that site effects contributed to the variations in ground motion observed throughout Christchurch city. We use densely-spaced aftershock recordings of the Darfield earthquake to investigate variations in local seismic site response within the Christchurch urban area. Following the Darfield main shock we deployed a temporary array of ~180 low-cost 14-bit MEMS accelerometers linked to the global Quake-Catcher Network (QCN). These instruments provided dense station coverage (spacing ~2 km) to complement existing New Zealand national network strong motion stations (GeoNet) within Christchurch city. Well-constrained standard spectral ratios were derived for GeoNet stations using a reference station on Miocene basalt rock in the south of the city. For noisier QCN stations, the method was adapted to find a maximum likelihood estimate of spectral ratio amplitude taking into account the variance of noise at the respective stations. Spectral ratios for QCN stations are similar to nearby GeoNet stations when the maximum likelihood method is used. Our study suggests dense low-cost accelerometer aftershock arrays can provide useful information on local-scale ground motion properties for use in microzonation. Preliminary results indicate higher amplifications north of the city centre and strong high-frequency amplification in the small, shallower basin of Heathcote Valley.

Slow-Frequency Pulsed Transcranial Electrical Stimulation for Modulation of Cortical Plasticity Based on Reciprocity Targeting with Precision Electrical Head Modeling

PubMed Central

Luu, Phan; Essaki Arumugam, Easwara Moorthy; Anderson, Erik; Gunn, Amanda; Rech, Dennis; Turovets, Sergei; Tucker, Don M.

2016-01-01

In pain management as well as other clinical applications of neuromodulation, it is important to consider the timing parameters influencing activity-dependent plasticity, including pulsed versus sustained currents, as well as the spatial action of electrical currents as they polarize the complex convolutions of the cortical mantle. These factors are of course related; studying temporal factors is not possible when the spatial resolution of current delivery to the cortex is so uncertain to make it unclear whether excitability is increased or decreased with anodal vs. cathodal current flow. In the present study we attempted to improve the targeting of specific cortical locations by applying current through flexible source-sink configurations of 256 electrodes in a geodesic array. We constructed a precision electric head model for 12 healthy individuals. Extraction of the individual’s cortical surface allowed computation of the component of the induced current that is normal to the target cortical surface. In an effort to replicate the long-term depression (LTD) induced with pulsed protocols in invasive animal research and transcranial magnetic stimulation studies, we applied 100 ms pulses at 1.9 s intervals either in cortical-surface-anodal or cortical-surface-cathodal directions, with a placebo (sham) control. The results showed significant LTD of the motor evoked potential as a result of the cortical-surface-cathodal pulses in contrast to the placebo control, with a smaller but similar LTD effect for anodal pulses. The cathodal LTD after-effect was sustained over 90 min following current injection. These results support the feasibility of pulsed protocols with low total charge in non-invasive neuromodulation when the precision of targeting is improved with a dense electrode array and accurate head modeling. PMID:27531976

Long-term implantation of deep brain stimulation electrodes in the pontine micturition centre of the Göttingen minipig.

PubMed

Jensen, Kristian N; Deding, Dorthe; Sørensen, Jens Christian; Bjarkam, Carsten R

2009-07-01

To implant deep brain stimulation (DBS) electrodes in the porcine pontine micturition centre (PMC) in order to establish a large animal model of PMC-DBS. Brain stems from four Göttingen minipigs were sectioned coronally into 40-mum-thick histological sections and stained with Nissl, auto-metallographic myelin stain, tyrosine hydroxylase and corticotrophin-releasing factor immunohistochemistry in order to identify the porcine PMC. DBS electrodes were then stereotaxically implanted on the right side into the PMC in four Göttingen minipigs, and the bladder response to electrical stimulation was evaluated by subsequent cystometry performed immediately after the operation and several weeks later. A paired CRF-dense area homologous to the PMC in other species was encountered in the rostral pontine tegmentum medial to the locus coeruleus and ventral to the floor of the fourth ventricle. Electrical stimulation of the CRF-dense area resulted in an increased detrusor pressure followed by visible voiding in some instances. The pigs were allowed to survive between 14 and 55 days, and electrical stimulation resulting in an increased detrusor pressure was performed on more than one occasion without affecting consciousness or general thriving. None of the pigs developed postoperative infections or died prematurely. DBS electrodes can be implanted for several weeks in the identified CRF-dense area resulting in a useful large animal model for basic research on micturition and the future clinical use of this treatment modality in neurogenic supra-pontine voiding disorders.

Localization of dense intracranial electrode arrays using magnetic resonance imaging

PubMed Central

Doyle, Werner K.; Halgren, Eric; Carlson, Chad; Belcher, Thomas L.; Cash, Sydney S.; Devinsky, Orrin; Thesen, Thomas

2013-01-01

Intracranial electrode arrays are routinely used in the pre-surgical evaluation of patients with medically refractory epilepsy, and recordings from these electrodes have been increasingly employed in human cognitive neurophysiology due to their high spatial and temporal resolution. For both researchers and clinicians, it is critical to localize electrode positions relative to the subject-specific neuroanatomy. In many centers, a post-implantation MRI is utilized for electrode detection because of its higher sensitivity for surgical complications and the absence of radiation. However, magnetic susceptibility artifacts surrounding each electrode prohibit unambiguous detection of individual electrodes, especially those that are embedded within dense grid arrays. Here, we present an efficient method to accurately localize intracranial electrode arrays based on pre- and post-implantation MR images that incorporates array geometry and the individual's cortical surface. Electrodes are directly visualized relative to the underlying gyral anatomy of the reconstructed cortical surface of individual patients. Validation of this approach shows high spatial accuracy of the localized electrode positions (mean of 0.96 mm±0.81 mm for 271 electrodes across 8 patients). Minimal user input, short processing time, and utilization of radiation-free imaging are strong incentives to incorporate quantitatively accurate localization of intracranial electrode arrays with MRI for research and clinical purposes. Co-registration to a standard brain atlas further allows inter-subject comparisons and relation of intracranial EEG findings to the larger body of neuroimaging literature. PMID:22759995

Tensor Toolbox for MATLAB v. 3.0

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kola, Tamara; Bader, Brett W.; Acar Ataman, Evrim NMN

Tensors (also known as multidimensional arrays or N-way arrays) are used in a variety of applications ranging from chemometrics to network analysis. The Tensor Toolbox provides classes for manipulating dense, sparse, and structured tensors using MATLAB's object-oriented features. It also provides algorithms for tensor decomposition and factorization, algorithms for computing tensor eigenvalues, and methods for visualization of results.

Radar Resource Management in a Dense Target Environment

DTIC Science & Technology

2014-03-01

problem faced by networked MFRs . While relaxing our assumptions concerning information gain presents numerous challenges worth exploring, future research...linear programming MFR multifunction phased array radar MILP mixed integer linear programming NATO North Atlantic Treaty Organization PDF probability...1: INTRODUCTION Multifunction phased array radars ( MFRs ) are capable of performing various tasks in rapid succession. The performance of target search

Carbon Nanofiber Nanoelectrodes for Neural Stimulation and Chemical Detection: The Era of "Smart" Deep Brain Stimulation

NASA Technical Reports Server (NTRS)

Koehne, Jessica E.

2016-01-01

A sensor platform based on vertically aligned carbon nanofibers (CNFs) has been developed. Their inherent nanometer scale, high conductivity, wide potential window, good biocompatibility and well-defined surface chemistry make them ideal candidates as biosensor electrodes. Here, we report two studies using vertically aligned CNF nanoelectrodes for biomedical applications. CNF arrays are investigated as neural stimulation and neurotransmitter recording electrodes for application in deep brain stimulation (DBS). Polypyrrole coated CNF nanoelectrodes have shown great promise as stimulating electrodes due to their large surface area, low impedance, biocompatibility and capacity for highly localized stimulation. CNFs embedded in SiO2 have been used as sensing electrodes for neurotransmitter detection. Our approach combines a multiplexed CNF electrode chip, developed at NASA Ames Research Center, with the Wireless Instantaneous Neurotransmitter Concentration Sensor (WINCS) system, developed at the Mayo Clinic. Preliminary results indicate that the CNF nanoelectrode arrays are easily integrated with WINCS for neurotransmitter detection in a multiplexed array format. In the future, combining CNF based stimulating and recording electrodes with WINCS may lay the foundation for an implantable smart therapeutic system that utilizes neurochemical feedback control while likely resulting in increased DBS application in various neuropsychiatric disorders. In total, our goal is to take advantage of the nanostructure of CNF arrays for biosensing studies requiring ultrahigh sensitivity, high-degree of miniaturization, and selective biofunctionalization.

Carbon Nanofiber Nanoelectrodes for Neural Stimulation and Chemical Detection: The Era of Smart Deep Brain Stimulation

NASA Technical Reports Server (NTRS)

Koehne, Jessica E.

2016-01-01

A sensor platform based on vertically aligned carbon nanofibers (CNFs) has been developed. Their inherent nanometer scale, high conductivity, wide potential window, good biocompatibility and well-defined surface chemistry make them ideal candidates as biosensor electrodes. Here, we report two studies using vertically aligned CNF nanoelectrodes for biomedical applications. CNF arrays are investigated as neural stimulation and neurotransmitter recording electrodes for application in deep brain stimulation (DBS). Polypyrrole coated CNF nanoelectrodes have shown great promise as stimulating electrodes due to their large surface area, low impedance, biocompatibility and capacity for highly localized stimulation. CNFs embedded in SiO2 have been used as sensing electrodes for neurotransmitter detection. Our approach combines a multiplexed CNF electrode chip, developed at NASA Ames Research Center, with the Wireless Instantaneous Neurotransmitter Concentration Sensor (WINCS) system, developed at the Mayo Clinic. Preliminary results indicate that the CNF nanoelectrode arrays are easily integrated with WINCS for neurotransmitter detection in a multiplexed array format. In the future, combining CNF based stimulating and recording electrodes with WINCS may lay the foundation for an implantable "smart" therapeutic system that utilizes neurochemical feedback control while likely resulting in increased DBS application in various neuropsychiatric disorders. In total, our goal is to take advantage of the nanostructure of CNF arrays for biosensing studies requiring ultrahigh sensitivity, high-degree of miniaturization, and selective biofunctionalization.

Ambient Vehicular Noise recorded on a 2D Distributed Fiber Optic Sensing Array :Applications to Permafrost Thaw Detection and Imaging

NASA Astrophysics Data System (ADS)

Ajo Franklin, J. B.; Lindsey, N.; Wagner, A. M.; Dou, S.; Martin, E. R.; Ekblaw, I.; Ulrich, C.; James, S. R.; Freifeld, B. M.; Daley, T. M.

2016-12-01

Distributed Acoustic Sensing (DAS) is a recently developed technique that allows the spatially dense ( 1m) continuous recording of seismic signals on long strands of commercial fiber optic cables. The availability of continuous recording on dense arrays offers unique possibilities for long-term timelapse monitoring of environmental processes in arctic environments. In the absence of a repeatable semi-permanent seismic source, the use of ambient surface wave noise from infrastructure use (e.g. moving vehicles) for seismic imaging allows tomographic monitoring of evolving subsurface systems. Challenges in such scenarios include (1) the processing requirements for dense (1000+ channel) arrays recording weeks to months of seismic data, (2) appropriate methods to retrieve empirical noise correlation functions (NCFs) in environments with non-optimal array geometries and both coherent as well as incoherent noise, and (3) semi-automated approaches to invert timelapse NCFs for near-surface soil properties.We present an exploratory study of data from a sparse 2D DAS array acquisition on 4000 linear meters of trenched fiber deployed in 10 crossing profiles. The dataset, collected during July and August of 2016, covers a zone of permafrost undergoing a controlled thaw induced by an array of resistive heaters. The site, located near a heavily used road, has a high level of infrastructure noise but exhibits distance-dependent variation in both noise amplitude and spectrum. We apply seismic interferometry to retrieve the empirical NCF across array subsections, and use collocated geophone and broadband sensors to measure the NCF against the true impulse response function of the medium. We demonstrate that the combination of vehicle tracking and data windowing allows improved reconstruction of stable NCFs appropriate for dispersion analysis and inversion. We also show both spatial and temporal patterns of background noise at the site using 2D beamforming and spectral analysis. Our results suggest that valuable information can be extracted from ambient noise recorded with DAS, particularly in the context of monitoring transformations in cold region environments.

Electrode array for neural stimulation

DOEpatents

Wessendorf, Kurt O [Albuquerque, NM; Okandan, Murat [Edgewood, NM; Stein, David J [Albuquerque, NM; Yang, Pin [Albuquerque, NM; Cesarano, III, Joseph; Dellinger, Jennifer [Albuquerque, NM

2011-08-16

An electrode array for neural stimulation is disclosed which has particular applications for use in a retinal prosthesis. The electrode array can be formed as a hermetically-sealed two-part ceramic package which includes an electronic circuit such as a demultiplexer circuit encapsulated therein. A relatively large number (up to 1000 or more) of individually-addressable electrodes are provided on a curved surface of a ceramic base portion the electrode array, while a much smaller number of electrical connections are provided on a ceramic lid of the electrode array. The base and lid can be attached using a metal-to-metal seal formed by laser brazing. Electrical connections to the electrode array can be provided by a flexible ribbon cable which can also be used to secure the electrode array in place.

Studies of infrasound propagation using the USArray seismic network (Invited)

NASA Astrophysics Data System (ADS)

Hedlin, M. A.; Degroot-Hedlin, C. D.; Walker, K. T.

2010-12-01

Although there are currently ~ 100 infrasound arrays worldwide, more than ever before, the station density is still insufficient to provide validation for detailed propagation modeling. Much structure in the atmosphere is short-lived and occurs at spatial scales much smaller than the average distance between infrasound stations. Relatively large infrasound signals can be observed on seismic channels due to coupling at the Earth's surface. Recent research, using data from the 70-km spaced 400-station USArray and other seismic network deployments, has shown the value of dense seismic network data for filling in the gaps between infrasound arrays. The dense sampling of the infrasound wavefield has allowed us to observe complete travel-time branches of infrasound signals and shed more light on the nature of infrasound propagation. We present early results from our studies of impulsive atmospheric sources, such as series of UTTR rocket motor detonations in Utah. The Utah blasts have been well recorded by USArray seismic stations and infrasound arrays in Nevada and Washington State. Recordings of seismic signals from a series of six events in 2007 are used to pinpoint the shot times to < 1 second. Variations in the acoustic branches and signal arrival times at the arrays are used to probe variations in atmospheric structure. Although we currently use coupled signals we anticipate studying dense acoustic network recordings as the USArray is currently being upgraded with infrasound microphones. These new sensors will allow us to make semi-continental scale network recordings of infrasound signals free of concerns about how the signals observed on seismic channels were modified when being coupled to seismic.

Platinum nanowire microelectrode arrays for neurostimulation applications: Fabrication, characterization, and in-vitro retinal cell stimulation

NASA Astrophysics Data System (ADS)

Whalen, John J., III

Implantable electrical neurostimulating devices are being developed for a number of applications, including artificial vision through retinal stimulation. The epiretinal prosthesis will use a two-dimensional array microelectrodes to address individual cells of the retina. MEMS fabrication processes can produce arrays of microelectrodes with these dimensions, but there are two critical issues that they cannot satisfy. One, the stimulating electrodes are the only part of the implanted electrical device that penetrate through the water impermeable package, and must do so without sacrificing hermeticity. Two, As electrode size decreases, the current density (A cm-2 ) increases, due to increased electrochemical impedance. This reduces the amount of charge that can be safely injected into the tissue. To date, MEMS processing method, cannot produce electrode arrays with good, prolonged hermetic properties. Similarly, MEMS approaches do not account for the increased impedance caused by decreased surface area. For these reasons there is a strong motivation for the development of a water-impermeable, substrate-penetrating electrode array with low electrochemical impedance. This thesis presents a stimulating electrode array fabricated from platinum nanowires using a modified electrochemical template synthesis approach. Nanowires are electrochemically deposited from ammonium hexachloroplatinate solution into lithographically patterned nanoporous anodic alumina templates to produce microarrays of platinum nanowires. The platinum nanowires penetrating through the ceramic aluminum oxide template serve as parallel electrical conduits through the water impermeable, electrically insulating substrate. Electrode impedance can be adjusted by either controlling the nanowire hydrous platinum oxide content or by partially etching the alumina template to expose additional surface area. A stepwise approach to this project was taken. First, the electrochemistry of ammonium hexachloroplatinate solution was characterized, and physical properties of electrodeposited thin films were correlated to deposition conditions used. Second, platinum nanowires were fabricated and their properties characterized, using similar deposition conditions. Third, the feasibility of fabricating platinum nanowire stimulating electrode arrays with a variety of surface structures was demonstrated. Fourth, the enhanced charge transfer characteristics of these structures were demonstrated using electrochemical techniques. Finally, retinal cell stimulation was demonstrated using electrodes from platinum nanowire arrays.

GFZ Wireless Seismic Array (GFZ-WISE), a Wireless Mesh Network of Seismic Sensors: New Perspectives for Seismic Noise Array Investigations and Site Monitoring

PubMed Central

Picozzi, Matteo; Milkereit, Claus; Parolai, Stefano; Jaeckel, Karl-Heinz; Veit, Ingo; Fischer, Joachim; Zschau, Jochen

2010-01-01

Over the last few years, the analysis of seismic noise recorded by two dimensional arrays has been confirmed to be capable of deriving the subsoil shear-wave velocity structure down to several hundred meters depth. In fact, using just a few minutes of seismic noise recordings and combining this with the well known horizontal-to-vertical method, it has also been shown that it is possible to investigate the average one dimensional velocity structure below an array of stations in urban areas with a sufficient resolution to depths that would be prohibitive with active source array surveys, while in addition reducing the number of boreholes required to be drilled for site-effect analysis. However, the high cost of standard seismological instrumentation limits the number of sensors generally available for two-dimensional array measurements (i.e., of the order of 10), limiting the resolution in the estimated shear-wave velocity profiles. Therefore, new themes in site-effect estimation research by two-dimensional arrays involve the development and application of low-cost instrumentation, which potentially allows the performance of dense-array measurements, and the development of dedicated signal-analysis procedures for rapid and robust estimation of shear-wave velocity profiles. In this work, we present novel low-cost wireless instrumentation for dense two-dimensional ambient seismic noise array measurements that allows the real–time analysis of the surface-wavefield and the rapid estimation of the local shear-wave velocity structure for site response studies. We first introduce the general philosophy of the new system, as well as the hardware and software that forms the novel instrument, which we have tested in laboratory and field studies. PMID:22319298

FM Dye Cycling at the Synapse: Comparing High Potassium Depolarization, Electrical and Channelrhodopsin Stimulation.

PubMed

Kopke, Danielle L; Broadie, Kendal

2018-05-24

FM dyes are used to study the synaptic vesicle (SV) cycle. These amphipathic probes have a hydrophilic head and hydrophobic tail, making them water-soluble with the ability to reversibly enter and exit membrane lipid bilayers. These styryl dyes are relatively non-fluorescent in aqueous medium, but insertion into the outer leaflet of the plasma membrane causes a >40X increase in fluorescence. In neuronal synapses, FM dyes are internalized during SV endocytosis, trafficked both within and between SV pools, and released with SV exocytosis, providing a powerful tool to visualize presynaptic stages of neurotransmission. A primary genetic model of glutamatergic synapse development and function is the Drosophila neuromuscular junction (NMJ), where FM dye imaging has been used extensively to quantify SV dynamics in a wide range of mutant conditions. The NMJ synaptic terminal is easily accessible, with a beautiful array of large synaptic boutons ideal for imaging applications. Here, we compare and contrast the three ways to stimulate the Drosophila NMJ to drive activity-dependent FM1-43 dye uptake/release: 1) bath application of high [K + ] to depolarize neuromuscular tissues, 2) suction electrode motor nerve stimulation to depolarize the presynaptic nerve terminal, and 3) targeted transgenic expression of channelrhodopsin variants for light-stimulated, spatial control of depolarization. Each of these methods has benefits and disadvantages for the study of genetic mutation effects on the SV cycle at the Drosophila NMJ. We will discuss these advantages and disadvantages to assist the selection of the stimulation approach, together with the methodologies specific to each strategy. In addition to fluorescent imaging, FM dyes can be photoconverted to electron-dense signals visualized using transmission electron microscopy (TEM) to study SV cycle mechanisms at an ultrastructural level. We provide the comparisons of confocal and electron microscopy imaging from the different methods of Drosophila NMJ stimulation, to help guide the selection of future experimental paradigms.

A Multi-Functional Microelectrode Array Featuring 59760 Electrodes, 2048 Electrophysiology Channels, Stimulation, Impedance Measurement and Neurotransmitter Detection Channels.

PubMed

Dragas, Jelena; Viswam, Vijay; Shadmani, Amir; Chen, Yihui; Bounik, Raziyeh; Stettler, Alexander; Radivojevic, Milos; Geissler, Sydney; Obien, Marie; Müller, Jan; Hierlemann, Andreas

2017-06-01

Biological cells are characterized by highly complex phenomena and processes that are, to a great extent, interdependent. To gain detailed insights, devices designed to study cellular phenomena need to enable tracking and manipulation of multiple cell parameters in parallel; they have to provide high signal quality and high spatiotemporal resolution. To this end, we have developed a CMOS-based microelectrode array system that integrates six measurement and stimulation functions, the largest number to date. Moreover, the system features the largest active electrode array area to date (4.48×2.43 mm 2 ) to accommodate 59,760 electrodes, while its power consumption, noise characteristics, and spatial resolution (13.5 μm electrode pitch) are comparable to the best state-of-the-art devices. The system includes: 2,048 action-potential (AP, bandwidth: 300 Hz to 10 kHz) recording units, 32 local-field-potential (LFP, bandwidth: 1 Hz to 300 Hz) recording units, 32 current recording units, 32 impedance measurement units, and 28 neurotransmitter detection units, in addition to the 16 dual-mode voltage-only or current/voltage-controlled stimulation units. The electrode array architecture is based on a switch matrix, which allows for connecting any measurement/stimulation unit to any electrode in the array and for performing different measurement/stimulation functions in parallel.

Low-cost silver capped polystyrene nanotube arrays as super-hydrophobic substrates for SERS applications.

PubMed

Lovera, Pierre; Creedon, Niamh; Alatawi, Hanan; Mitchell, Micki; Burke, Micheal; Quinn, Aidan J; O'Riordan, Alan

2014-05-02

In this paper, we describe the fabrication, simulation and characterization of dense arrays of freestanding silver capped polystyrene nanotubes, and demonstrate their suitability for surface enhanced Raman scattering (SERS) applications. Substrates are fabricated in a rapid, low-cost and scalable way by melt wetting of polystyrene (PS) in an anodized alumina (AAO) template, followed by silver evaporation. Scanning electron microscopy reveals that substrates are composed of a dense array of freestanding polystyrene nanotubes topped by silver nanocaps. SERS characterization of the substrates, employing a monolayer of 4-aminothiophenol (4-ABT) as a model molecule, exhibits an enhancement factor of ∼1.6 × 10(6), in agreement with 3D finite difference time domain simulations. Contact angle measurements of the substrates revealed super-hydrophobic properties, allowing pre-concentration of target analyte into a small volume. These super-hydrophobic properties of the samples are taken advantage of for sensitive detection of the organic pollutant crystal violet, with detection down to ∼400 ppt in a 2 μl aliquot demonstrated.

Size and space controlled hexagonal arrays of superparamagnetic iron oxide nanodots: magnetic studies and application

PubMed Central

Ghoshal, Tandra; Maity, Tuhin; Senthamaraikannan, Ramsankar; Shaw, Matthew T.; Carolan, Patrick; Holmes, Justin D.; Roy, Saibal; Morris, Michael A.

2013-01-01

Highly dense hexagonally arranged iron oxide nanodots array were fabricated using PS-b-PEO self-assembled patterns. The copolymer molecular weight, composition and choice of annealing solvent/s allows dimensional and structural control of the nanopatterns at large scale. A mechanism is proposed to create scaffolds through degradation and/or modification of cylindrical domains. A methodology based on selective metal ion inclusion and subsequent processing was used to create iron oxide nanodots array. The nanodots have uniform size and shape and their placement mimics the original self-assembled nanopatterns. For the first time these precisely defined and size selective systems of ordered nanodots allow careful investigation of magnetic properties in dimensions from 50 nm to 10 nm, which delineate the nanodots are superparamagnetic, well-isolated and size monodispersed. This diameter/spacing controlled iron oxide nanodots systems were demonstrated as a resistant mask over silicon to fabricate densely packed, identical ordered, high aspect ratio silicon nanopillars and nanowire features. PMID:24072037

A Highly Efficient Sensor Platform Using Simply Manufactured Nanodot Patterned Substrates

PubMed Central

Rasappa, Sozaraj; Ghoshal, Tandra; Borah, Dipu; Senthamaraikannan, Ramsankar; Holmes, Justin D.; Morris, Michael A.

2015-01-01

Block copolymer (BCP) self-assembly is a low-cost means to nanopattern surfaces. Here, we use these nanopatterns to directly print arrays of nanodots onto a conducting substrate (Indium Tin Oxide (ITO) coated glass) for application as an electrochemical sensor for ethanol (EtOH) and hydrogen peroxide (H2O2) detection. The work demonstrates that BCP systems can be used as a highly efficient, flexible methodology for creating functional surfaces of materials. Highly dense iron oxide nanodots arrays that mimicked the original BCP pattern were prepared by an ‘insitu’ BCP inclusion methodology using poly(styrene)-block-poly(ethylene oxide) (PS-b-PEO). The electrochemical behaviour of these densely packed arrays of iron oxide nanodots fabricated by two different molecular weight PS-b-PEO systems was studied. The dual detection of EtOH and H2O2 was clearly observed. The as-prepared nanodots have good long term thermal and chemical stability at the substrate and demonstrate promising electrocatalytic performance. PMID:26290188

Towards Integrated Marmara Strong Motion Network

NASA Astrophysics Data System (ADS)

Durukal, E.; Erdik, M.; Safak, E.; Ansal, A.; Ozel, O.; Alcik, H.; Mert, A.; Kafadar, N.; Korkmaz, A.; Kurtulus, A.

2009-04-01

Istanbul has a 65% chance of having a magnitude 7 or above earthquake within the next 30 years. As part of the preparations for the future earthquake, strong motion networks have been installed in and around Istanbul. The Marmara Strong Motion Network, operated by the Department of Earthquake Engineering of Kandilli Observatory and Earthquake Research Institute, encompasses permanent systems outlined below. It is envisaged that the networks will be run by a single entity responsible for technical management and maintanence, as well as for data management, archiving and dissemination through dedicated web-based interfaces. • Istanbul Earthquake Rapid Response and Early Warning System - IERREWS (one hundred 18-bit accelerometers for rapid response; ten 24-bit accelerometers for early warning) • IGDAŞ Gas Shutoff Network (100 accelerometers to be installed in 2010 and integrated with IERREWS) • Structural Monitoring Arrays - Fatih Sultan Mehmet Suspension Bridge (1200m-long suspension bridge across the Bosphorus, five 3-component accelerometers + GPS sensors) - Hagia Sophia Array (1500-year-old historical edifice, 9 accelerometers) - Süleymaniye Mosque Array (450-year-old historical edifice,9 accelerometers) - Fatih Mosque Array (237-year-old historical edifice, 9 accelerometers) - Kanyon Building Array (high-rise office building, 5 accelerometers) - Isbank Tower Array (high-rise office building, 5 accelerometers) - ENRON Array (power generation facility, 4 acelerometers) - Mihrimah Sultan Mosque Array (450-year-old historical edifice,9 accelerometers + tiltmeters, to be installed in 2009) - Sultanahmet Mosque Array, (390-year-old historical edifice, 9 accelerometers + tiltmeters, to be installed in 2009) • Special Arrays - Atakoy Vertical Array (four 3-component accelerometers at 25, 50, 75, and 150 m depths) - Marmara Tube Tunnel (1400 m long submerged tunnel, 128 ch. accelerometric data, 24 ch. strain data, to be installed in 2010) - Air-Force Academy Array (72 ch. dense accelerometric array to be installed in 2010) - Gemlik Array (a dense basin array of 8 stations, to be installed in 2010) The objectives of these systems and networks are: (1) to produce rapid earthquake intensity, damage and loss assessment information after an earthquake (in the case of IERREWS), (2) to monitor conditions of structural systems, (3) to develop real-time data processing, analysis, and damage detection and location tools (in the case of structural networks) after an extreme event, (4) to assess spatial properties of strong ground motion and ground strain, and to characterise basin response (in the case of special arrays), (5) to investigate site response and wave propagation (in the case of vertical array). Ground motion data obtained from these strong motion networks have and are being used for investigations of attenuation, spatial variation (coherence), simulation benchmarking, source modeling, site response, seismic microzonation, system identification and structural model verification and structural health control. In addition to the systems and networks outlined above there are two temporary networks: KIMNET - a dense urban noise and microtremor network consisting of 50 broadband stations expected to be operational in mid 2009, and SOSEWIN - a 20-station, self-organizing structural integrated array at Ataköy in Istanbul.

Uptake and economic impact of first-cycle colony-stimulating factor use during adjuvant treatment of breast cancer.

PubMed

Hershman, Dawn L; Wilde, Elizabeth T; Wright, Jason D; Buono, Donna L; Kalinsky, Kevin; Malin, Jennifer L; Neugut, Alfred I

2012-03-10

In 2002, pegfilgrastim was approved by the US Food and Drug Administration and the benefits of dose-dense breast cancer chemotherapy, especially for hormone receptor (HR) -negative tumors, were reported. We examined first-cycle colony-stimulating factor use (FC-CSF) before and after 2002 and estimated US expenditures for dose-dense chemotherapy. We identified patients in Surveillance, Epidemiology, and End Results-Medicare greater than 65 years old with stages I to III breast cancer who had greater than one chemotherapy claim within 6 months of diagnosis(1998 to 2005) and classified patients with an average cycle length less than 21 days as having received dose-dense chemotherapy. The associations of patient, tumor, and physician-related factors with the receipt of any colony-stimulating factor (CSF) and FC-CSF use were analyzed by using generalized estimating equations. CSF costs were estimated for patients who were undergoing dose-dense chemotherapy. Among the 10,773 patients identified, 5,266 patients (48.9%) had a CSF claim. CSF use was stable between 1998 and 2002 and increased from 36.8% to 73.7% between 2002 and 2005, FC-CSF use increased from 13.2% to 67.9%, and pegfilgrastim use increased from 4.1% to 83.6%. In a multivariable analysis, CSF use was associated with age and chemotherapy type and negatively associated with black/Hispanic race, rural residence, and shorter chemotherapy duration. FC-CSF use was associated with high socioeconomic status but not with age or race/ethnicity. The US annual CSF expenditure for women with HR-positive tumors treated with dose-dense chemotherapy is estimated to be $38.8 million. A rapid increase in FC-CSF use occurred over a short period of time, which was likely a result of the reported benefits of dose-dense chemotherapy and the ease of pegfilgrastim administration. Because of the increasing evidence that elderly HR-positive patients do not benefit from dose-dense chemotherapy, limiting pegfilgrastim use would combat the increasing costs of cancer care.

Chronic, percutaneous connector for electrical recording and stimulation with microelectrode arrays.

PubMed

Shah, Kedar G; Lee, Kye Young; Tolosa, Vanessa; Tooker, Angela; Felix, Sarah; Benett, William; Pannu, Satinderpall

2014-01-01

The translation of advances in neural stimulation and recording research into clinical practice hinges on the ability to perform chronic experiments in awake and behaving animal models. Advances in microelectrode array technology, most notably flexible polymer arrays, have significantly improved reliability of the neural interface. However, electrical connector technology has lagged and is prone to failure from non-biocompatibility, large size, contamination, corrosion, and difficulty of use. We present a novel chronic, percutaneous electrical connector system that is suitable for neural stimulation and recording. This system features biocompatible materials, low connect and disconnect forces, passive alignment, and a protective cap during non-use. We have successfully designed, assembled, and tested in vitro both a 16-channel system and a high density 64-channel system. Custom, polyimide, 16-channel, microelectrode arrays were electrically assembled with the connector system and tested using cyclic voltammetry and electrochemical impedance spectroscopy. This connector system is versatile and can be used with a variety of microelectrode array technologies for chronic studies.

Separating light absorption layer from channel in ZnO vertical nanorod arrays based photodetectors for high-performance image sensors

NASA Astrophysics Data System (ADS)

Ma, Yang; Wu, Congjun; Xu, Zhihao; Wang, Fei; Wang, Min

2018-05-01

Photoconductor arrays with both high responsivity and large ON/OFF ratios are of great importance for the application of image sensors. Herein, a ZnO vertical nanorod array based photoconductor with a light absorption layer separated from the device channel has been designed, in which the photo-generated carriers along the axial ZnO nanorods drive to the external electrodes through nanorod-nanorod junctions in the dense layer at the bottom. This design allows us to enhance the photocurrent with unchanged dark current by increasing the ratio between the ZnO nanorod length and the thickness of the dense layer to achieve both high responsivity and large ON/OFF ratios. As a result, the as-fabricated devices possess a high responsivity of 1.3 × 105 A/W, a high ON/OFF ratio of 790, a high detectivity of 1.3 × 1013 Jones, and a low detectable light intensity of 1 μW/cm2. More importantly, the developed approach enables the integration of ZnO vertical nanorod array based photodetectors as image sensors with uniform device-to-device performance.

Munc13 controls the location and efficiency of dense-core vesicle release in neurons.

PubMed

van de Bospoort, Rhea; Farina, Margherita; Schmitz, Sabine K; de Jong, Arthur; de Wit, Heidi; Verhage, Matthijs; Toonen, Ruud F

2012-12-10

Neuronal dense-core vesicles (DCVs) contain diverse cargo crucial for brain development and function, but the mechanisms that control their release are largely unknown. We quantified activity-dependent DCV release in hippocampal neurons at single vesicle resolution. DCVs fused preferentially at synaptic terminals. DCVs also fused at extrasynaptic sites but only after prolonged stimulation. In munc13-1/2-null mutant neurons, synaptic DCV release was reduced but not abolished, and synaptic preference was lost. The remaining fusion required prolonged stimulation, similar to extrasynaptic fusion in wild-type neurons. Conversely, Munc13-1 overexpression (M13OE) promoted extrasynaptic DCV release, also without prolonged stimulation. Thus, Munc13-1/2 facilitate DCV fusion but, unlike for synaptic vesicles, are not essential for DCV release, and M13OE is sufficient to produce efficient DCV release extrasynaptically.

A Wireless Implantable Switched-Capacitor Based Optogenetic Stimulating System

PubMed Central

Lee, Hyung-Min; Kwon, Ki-Yong; Li, Wen

2015-01-01

This paper presents a power-efficient implantable optogenetic interface using a wireless switched-capacitor based stimulating (SCS) system. The SCS efficiently charges storage capacitors directly from an inductive link and periodically discharges them into an array of micro-LEDs, providing high instantaneous power without affecting wireless link and system supply voltage. A custom-designed computer interface in LabVIEW environment wirelessly controls stimulation parameters through the inductive link, and an optrode array enables simultaneous neural recording along with optical stimulation. The 4-channel SCS system prototype has been implemented in a 0.35-μm CMOS process and combined with the optrode array. In vivo experiments involving light-induced local field potentials verified the efficacy of the SCS system. An implantable version of the SCS system with flexible hermetic sealing is under development for chronic experiments. PMID:25570099

Channel length scaling behavior in transistors based on individual versus dense arrays of carbon nanotubes

NASA Astrophysics Data System (ADS)

Brady, Gerald J.; Jinkins, Katherine R.; Arnold, Michael S.

2017-09-01

Recent advances in the solution-phase sorting and assembly of semiconducting single-walled carbon nanotubes (SWCNTs) have enabled significant gains in the performance of field-effect transistors (FETs) constructed from dense arrays of aligned SWCNTs. However, the channel length (LCH) downscaling behaviors of these arrays, which contain some organizational disorder (i.e., rotational misalignment and non-uniform pitch), have not yet been studied in detail below LCH of 100 nm. This study compares the behaviors of individualized SWCNTs with arrays of aligned, solution-cast SWCNTs in FETs with LCH ranging from 30 to 240 nm. The on-state conductance of both individual and array SWCNTs rises with decreasing LCH. Nearly ballistic transport is observed for LCH < 40 nm in both cases, reaching a conductance of 0.82 Go per SWCNT in arrays, where Go = 2e2/h is the quantum conductance. In the off-state, the off-current and subthreshold swing of the individual SWCNTs remain nearly invariant with decreasing LCH whereas array SWCNT FETs suffer from increasing off-state current and deteriorating subthreshold swing for LCH below 100 nm. We analyze array disorder using atomic force microscopy, which shows that crossing SWCNTs that arise from misoriented alignment raise SWCNTs off of the substrate for large portions of the channel when LCH is small. Electrostatics modeling analysis indicates that these raised SWCNTs are a likely contributor to the deteriorating off-current and subthreshold characteristics of arrays. These results demonstrate that improved inter-SWCNT pitch uniformity and alignment with minimal inter-SWCNT interactions will be necessary in order for solution processed SWCNT arrays to reach subthreshold performance on par with isolated SWCNTs. These results are also promising because they show that arrays of solution-processed SWCNTs can nearly reach ballistic conductance in the on-state despite imperfections in pitch and alignment.

A versatile all-channel stimulator for electrode arrays, with real-time control

PubMed Central

Wagenaar, Daniel A; Potter, Steve M

2008-01-01

Over the last few decades, technology to record through ever increasing numbers of electrodes has become available to electrophysiologists. For the study of distributed neural processing, however, the ability to stimulate through equal numbers of electrodes, and thus to attain bidirectional communication, is of paramount importance. Here, we present a stimulation system for multi-electrode arrays which interfaces with existing commercial recording hardware, and allows stimulation through any electrode in the array, with rapid switching between channels. The system is controlled through real-time Linux, making it extremely flexible: stimulation sequences can be constructed on-the-fly, and arbitrary stimulus waveforms can be used if desired. A key feature of this design is that it can be readily and inexpensively reproduced in other labs, since it interfaces to standard PC parallel ports and uses only off-the-shelf components. Moreover, adaptation for use with in vivo multi-electrode probes would be straightforward. In combination with our freely available data-acquisition software, MeaBench, this system can provide feedback stimulation in response to recorded action potentials within 15 ms. PMID:15876621

The 3-dimensional grid: a novel approach to stereoelectroencephalography.

PubMed

Munyon, Charles; Sweet, Jennifer; Luders, Hans; Lhatoo, Samden; Miller, Jonathan

2015-03-01

Successful surgical treatment of epilepsy requires accurate definition of areas of ictal onset and eloquent brain. Although invasive monitoring can help, subdural grids cannot sample sulci or subcortical tissue; traditional stereoelectroencephalography depth electrodes are usually placed too far apart to provide sufficient resolution for mapping. To report a strategy of depth electrode placement in a dense array to allow precise anatomic localization of epileptic and eloquent cortex. Twenty patients with medically intractable epilepsy either poorly localized or found to arise adjacent to eloquent areas underwent placement of arrays of depth electrodes into and around the putative area of seizure onset with the use of framed stereotaxy. Each array consisted of a "grid" of parallel electrodes in a rectangular pattern with 1 cm between entry sites. In a subset of patients, a few electrodes were placed initially, with additional electrodes placed in a second stage. Trajectories were modified to avoid cortical vessels defined on magnetic resonance imaging. Patients were monitored for 4 to 21 days to establish the precise location of seizure onset. Stimulation was performed to map cortical and subcortical eloquent regions. Electrode locations were coregistered for frameless stereotaxy during subsequent resection of seizure focus. Two hundred fifty-four electrodes were implanted. Discrete regions of seizure onset and functional cortex were identified, which were used during resection to remove epileptogenic tissue while preserving eloquent areas. There were no hemorrhagic or infectious complications; no patient suffered permanent neurological deficit. The 3-dimensional intraparenchymal grid is useful for identifying the location and extent of epileptic and eloquent brain.

Growth of large-scale nanotwinned Cu nanowire arrays from anodic aluminum oxide membrane by electrochemical deposition process: controllable nanotwin density and growth orientation with enhanced electrical endurance performance

NASA Astrophysics Data System (ADS)

Chan, Tsung-Cheng; Lin, Yen-Miao; Tsai, Hung-Wei; Wang, Zhiming M.; Liao, Chien-Neng; Chueh, Yu-Lun

2014-06-01

Densely nanotwinned Cu nanowire (NW) arrays with an identical diameter of ~55 nm were fabricated by pulse electrochemical deposition at low temperature using anodic aluminum oxide as a template. Different growth orientations of nanotwinned Cu nanowire arrays were investigated. The endurance of the electrical current density before breakdown of the nanotwinned Cu NWs can reach up to 2.4 × 108 A cm-2. The formation of highly dense nanotwins is attributed to relaxation of coalescence induced stress and twin fault stacking when Cu NWs grow by two-dimensional kinetics. A mechanism based on the twinning structure effect on the electromigration was proposed to explain the improved electrical endurance of Cu. The result demonstrates that the formation of nanotwins into Cu NWs can effectively suppress the void growth, leading to extended life time for use in electronic devices.Densely nanotwinned Cu nanowire (NW) arrays with an identical diameter of ~55 nm were fabricated by pulse electrochemical deposition at low temperature using anodic aluminum oxide as a template. Different growth orientations of nanotwinned Cu nanowire arrays were investigated. The endurance of the electrical current density before breakdown of the nanotwinned Cu NWs can reach up to 2.4 × 108 A cm-2. The formation of highly dense nanotwins is attributed to relaxation of coalescence induced stress and twin fault stacking when Cu NWs grow by two-dimensional kinetics. A mechanism based on the twinning structure effect on the electromigration was proposed to explain the improved electrical endurance of Cu. The result demonstrates that the formation of nanotwins into Cu NWs can effectively suppress the void growth, leading to extended life time for use in electronic devices. Electronic supplementary information (ESI) available: X-ray diffraction spectra of Cu NWs grown by electrochemical deposition with a current density of 1.5 A cm-2 at -1 °C and room temperature; bright-field TEM images of Cu NWs deposited at -1 °C with a current density of 0.4, 0.8, 1.8, and 1.5 A cm-2, respectively; illustration of the effect of twin density on the MTTF of Cu NWs. See DOI: 10.1039/c3nr06194a

Field Emission Study of Carbon Nanotubes: High Current Density from Nanotube Bundle Arrays

NASA Technical Reports Server (NTRS)

Bronikowski, Micheal J.; Manohara, Harish M.; Siegel, Peter H.; Hunt, Brian D.

2004-01-01

We have investigated the field emission behavior of lithographically patterned bundles of multiwalled carbon nanotubes arranged in a variety of array geometries. Such arrays of nanotube bundles are found to perform significantly better in field emission than arrays of isolated nanotubes or dense, continuous mats of nanotubes, with the field emission performance depending on the bundle diameter and inter-bundle spacing. Arrays of 2-micrometers diameter nanotube bundles spaced 5 micrometers apart (edge-to-edge spacing) produced the largest emission densities, routinely giving 1.5 to 1.8 A/cm(sup 2) at approximately 4 V/micrometer electric field, and greater than 6 A/cm(sup 2) at 20 V/micrometers.

A Low-Cost Energy-Efficient Cableless Geophone Unit for Passive Surface Wave Surveys.

PubMed

Dai, Kaoshan; Li, Xiaofeng; Lu, Chuan; You, Qingyu; Huang, Zhenhua; Wu, H Felix

2015-09-25

The passive surface wave survey is a practical, non-invasive seismic exploration method that has increasingly been used in geotechnical engineering. However, in situ deployment of traditional wired geophones is labor intensive for a dense sensor array. Alternatively, stand-alone seismometers can be used, but they are bulky, heavy, and expensive because they are usually designed for long-term monitoring. To better facilitate field applications of the passive surface wave survey, a low-cost energy-efficient geophone system was developed in this study. The hardware design is presented in this paper. To validate the system's functionality, both laboratory and field experiments were conducted. The unique feature of this newly-developed cableless geophone system allows for rapid field applications of the passive surface wave survey with dense array measurements.

A fast stimulability screening protocol for neuronal cultures on microelectrode arrays.

PubMed

Kapucu, Fikret E; Tanskanen, Jarno M A; Yuan, Yuting; Hyttinen, Jari A K

2015-01-01

Microelectrode arrays (MEAs) are used to study the electrical activity in brain slices and neuronal cultures. MEA experiments for the analysis of electrical stimulation responses require the tissue or culture to be prone to stimulation. For brain slices, potential stimulation sites may be directly visible in microscope, in which case the determination of stimulability at those locations is sufficient. In unstructured neuronal cultures, potential stimulation sites may not be known a priori, and spatial stimulability screening should be performed. Considering, e.g., 59 microelectrode sites, each to be stimulated several times, may result in long screening times, unacceptable with a MEA system without an integrated CO2 incubator, or in high stimulation effects on the networks. Here, we describe an implementation of a fast stimulation protocol employing pseudorandom stimulation site switching aiming at alleviating the network effects of the stimulability screening. In this paper, we show the usability of the proposed protocol by first detecting stimulable locations and subsequently apply repeated stimulation on the identified potentially stimulable locations to observe an exemplary neuronal pathway.

Performance metrics for state-of-the-art airborne magnetic and electromagnetic systems for mapping and detection of unexploded ordnance

NASA Astrophysics Data System (ADS)

Doll, William E.; Bell, David T.; Gamey, T. Jeffrey; Beard, Les P.; Sheehan, Jacob R.; Norton, Jeannemarie

2010-04-01

Over the past decade, notable progress has been made in the performance of airborne geophysical systems for mapping and detection of unexploded ordnance in terrestrial and shallow marine environments. For magnetometer systems, the most significant improvements include development of denser magnetometer arrays and vertical gradiometer configurations. In prototype analyses and recent Environmental Security Technology Certification Program (ESTCP) assessments using new production systems the greatest sensitivity has been achieved with a vertical gradiometer configuration, despite model-based survey design results which suggest that dense total-field arrays would be superior. As effective as magnetometer systems have proven to be at many sites, they are inadequate at sites where basalts and other ferrous geologic formations or soils produce anomalies that approach or exceed those of target ordnance items. Additionally, magnetometer systems are ineffective where detection of non-ferrous ordnance items is of primary concern. Recent completion of the Battelle TEM-8 airborne time-domain electromagnetic system represents the culmination of nearly nine years of assessment and development of airborne electromagnetic systems for UXO mapping and detection. A recent ESTCP demonstration of this system in New Mexico showed that it was able to detect 99% of blind-seeded ordnance items, 81mm and larger, and that it could be used to map in detail a bombing target on a basalt flow where previous airborne magnetometer surveys had failed. The probability of detection for the TEM-8 in the blind-seeded study area was better than that reported for a dense-array total-field magnetometer demonstration of the same blind-seeded site, and the TEM-8 system successfully detected these items with less than half as many anomaly picks as the dense-array total-field magnetometer system.

Eviction of linker histone H1 by NAP-family histone chaperones enhances activated transcription.

PubMed

Zhang, Qian; Giebler, Holli A; Isaacson, Marisa K; Nyborg, Jennifer K

2015-01-01

In the Metazoan nucleus, core histones assemble the genomic DNA to form nucleosome arrays, which are further compacted into dense chromatin structures by the linker histone H1. The extraordinary density of chromatin creates an obstacle for accessing the genetic information. Regulation of chromatin dynamics is therefore critical to cellular homeostasis, and histone chaperones serve as prominent players in these processes. In the current study, we examined the role of specific histone chaperones in negotiating the inherently repressive chromatin structure during transcriptional activation. Using a model promoter, we demonstrate that the human nucleosome assembly protein family members hNap1 and SET/Taf1β stimulate transcription in vitro during pre-initiation complex formation, prior to elongation. This stimulatory effect is dependent upon the presence of activators, p300, and Acetyl-CoA. We show that transcription from our chromatin template is strongly repressed by H1, and that both histone chaperones enhance RNA synthesis by overcoming H1-induced repression. Importantly, both hNap1 and SET/Taf1β directly bind H1, and function to enhance transcription by evicting the linker histone from chromatin reconstituted with H1. In vivo studies demonstrate that SET/Taf1β, but not hNap1, strongly stimulates activated transcription from the chromosomally-integrated model promoter, consistent with the observation that SET/Taf1β is nuclear, whereas hNap1 is primarily cytoplasmic. Together, these observations indicate that SET/Taf1β may serve as a critical regulator of H1 dynamics and gene activation in vivo. These studies uncover a novel function for SET that mechanistically couples transcriptional derepression with H1 dynamics. Furthermore, they underscore the significance of chaperone-dependent H1 displacement as an essential early step in the transition of a promoter from a dense chromatin state into one that is permissive to transcription factor binding and robust activation.

Carbon Nanofiber Nanoelectrodes for Biosensing Applications

NASA Technical Reports Server (NTRS)

Koehne, Jessica Erin

2014-01-01

A sensor platform based on vertically aligned carbon nanofibers (CNFs) has been developed. Their inherent nanometer scale, high conductivity, wide potential window, good biocompatibility and well-defined surface chemistry make them ideal candidates as biosensor electrodes. Here, we report two studies using vertically aligned CNF nanoelectrodes for biomedical applications. CNF arrays are investigated as neural stimulation and neurotransmitter recording electrodes for application in deep brain stimulation (DBS). Polypyrrole coated CNF nanoelectrodes have shown great promise as stimulating electrodes due to their large surface area, low impedance, biocompatibility and capacity for highly localized stimulation. CNFs embedded in SiO2 have been used as sensing electrodes for neurotransmitter detection. Our approach combines a multiplexed CNF electrode chip, developed at NASA Ames Research Center, with the Wireless Instantaneous Neurotransmitter Concentration Sensor (WINCS) system, developed at the Mayo Clinic. Preliminary results indicate that the CNF nanoelectrode arrays are easily integrated with WINCS for neurotransmitter detection in a multiplexed array format. In the future, combining CNF based stimulating and recording electrodes with WINCS may lay the foundation for an implantable smart therapeutic system that utilizes neurochemical feedback control while likely resulting in increased DBS application in various neuropsychiatric disorders. In total, our goal is to take advantage of the nanostructure of CNF arrays for biosensing studies requiring ultrahigh sensitivity, high-degree of miniaturization, and selective biofunctionalization.

CMOS-micromachined, two-dimenisional transistor arrays for neural recording and stimulation.

PubMed

Lin, J S; Chang, S R; Chang, C H; Lu, S C; Chen, H

2007-01-01

In-plane microelectrode arrays have proven to be useful tools for studying the connectivities and the functions of neural tissues. However, seldom microelectrode arrays are monolithically-integrated with signal-processing circuits, without which the maximum number of electrodes is limited by the compromise with routing complexity and interferences. This paper proposes a CMOS-compatible, two-dimensional array of oxide-semiconductor field-effect transistors(OSFETs), capable of both recording and stimulating neuronal activities. The fabrication of the OSFETs not only requires simply die-level, post-CMOS micromachining process, but also retains metal layers for monolithic integration with signal-processing circuits. A CMOS microsystem containing the OSFET arrays and gain-programmable recording circuits has been fabricated and tested. The preliminary testing results are presented and discussed.

Estimation and partitioning of (co)heritability of inflammatory bowel disease from GWAS and immunochip data.

PubMed

Chen, Guo-Bo; Lee, Sang Hong; Brion, Marie-Jo A; Montgomery, Grant W; Wray, Naomi R; Radford-Smith, Graham L; Visscher, Peter M

2014-09-01

As custom arrays are cheaper than generic GWAS arrays, larger sample size is achievable for gene discovery. Custom arrays can tag more variants through denser genotyping of SNPs at associated loci, but at the cost of losing genome-wide coverage. Balancing this trade-off is important for maximizing experimental designs. We quantified both the gain in captured SNP-heritability at known candidate regions and the loss due to imperfect genome-wide coverage for inflammatory bowel disease using immunochip (iChip) and imputed GWAS data on 61,251 and 38.550 samples, respectively. For Crohn's disease (CD), the iChip and GWAS data explained 19 and 26% of variation in liability, respectively, and SNPs in the densely genotyped iChip regions explained 13% of the SNP-heritability for both the iChip and GWAS data. For ulcerative colitis (UC), the iChip and GWAS data explained 15 and 19% of variation in liability, respectively, and the dense iChip regions explained 10 and 9% of the SNP-heritability in the iChip and the GWAS data. From bivariate analyses, estimates of the genetic correlation in risk between CD and UC were 0.75 (SE 0.017) and 0.62 (SE 0.042) for the iChip and GWAS data, respectively. We also quantified the SNP-heritability of genomic regions that did or did not contain the previous 163 GWAS hits for CD and UC, and SNP-heritability of the overlapping loci between the densely genotyped iChip regions and the 163 GWAS hits. For both diseases, over different genomic partitioning, the densely genotyped regions on the iChip tagged at least as much variation in liability as in the corresponding regions in the GWAS data, however a certain amount of tagged SNP-heritability in the GWAS data was lost using the iChip due to the low coverage at unselected regions. These results imply that custom arrays with a GWAS backbone will facilitate more gene discovery, both at associated and novel loci. © The Author 2014. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Feasibility study of a take-home array-based functional electrical stimulation system with automated setup for current functional electrical stimulation users with foot-drop.

PubMed

Prenton, Sarah; Kenney, Laurence P; Stapleton, Claire; Cooper, Glen; Reeves, Mark L; Heller, Ben W; Sobuh, Mohammad; Barker, Anthony T; Healey, Jamie; Good, Timothy R; Thies, Sibylle B; Howard, David; Williamson, Tracey

2014-10-01

To investigate the feasibility of unsupervised community use of an array-based automated setup functional electrical stimulator for current foot-drop functional electrical stimulation (FES) users. Feasibility study. Gait laboratory and community use. Participants (N=7) with diagnosis of unilateral foot-drop of central neurologic origin (>6mo) who were regular users of a foot-drop FES system (>3mo). Array-based automated setup FES system for foot-drop (ShefStim). Logged usage, logged automated setup times for the array-based automated setup FES system and diary recording of problems experienced, all collected in the community environment. Walking speed, ankle angles at initial contact, foot clearance during swing, and the Quebec User Evaluation of Satisfaction with Assistive Technology version 2.0 (QUEST version 2.0) questionnaire, all collected in the gait laboratory. All participants were able to use the array-based automated setup FES system. Total setup time took longer than participants' own FES systems, and automated setup time was longer than in a previous study of a similar system. Some problems were experienced, but overall, participants were as satisfied with this system as their own FES system. The increase in walking speed (N=7) relative to no stimulation was comparable between both systems, and appropriate ankle angles at initial contact (N=7) and foot clearance during swing (n=5) were greater with the array-based automated setup FES system. This study demonstrates that an array-based automated setup FES system for foot-drop can be successfully used unsupervised. Despite setup's taking longer and some problems, users are satisfied with the system and it would appear as effective, if not better, at addressing the foot-drop impairment. Further product development of this unique system, followed by a larger-scale and longer-term study, is required before firm conclusions about its efficacy can be reached. Copyright © 2014 American Congress of Rehabilitation Medicine. Published by Elsevier Inc. All rights reserved.

High performance architecture design for large scale fibre-optic sensor arrays using distributed EDFAs and hybrid TDM/DWDM

NASA Astrophysics Data System (ADS)

Liao, Yi; Austin, Ed; Nash, Philip J.; Kingsley, Stuart A.; Richardson, David J.

2013-09-01

A distributed amplified dense wavelength division multiplexing (DWDM) array architecture is presented for interferometric fibre-optic sensor array systems. This architecture employs a distributed erbium-doped fibre amplifier (EDFA) scheme to decrease the array insertion loss, and employs time division multiplexing (TDM) at each wavelength to increase the number of sensors that can be supported. The first experimental demonstration of this system is reported including results which show the potential for multiplexing and interrogating up to 4096 sensors using a single telemetry fibre pair with good system performance. The number can be increased to 8192 by using dual pump sources.

Response profiles of murine spiral ganglion neurons on multi-electrode arrays

NASA Astrophysics Data System (ADS)

Hahnewald, Stefan; Tscherter, Anne; Marconi, Emanuele; Streit, Jürg; Widmer, Hans Rudolf; Garnham, Carolyn; Benav, Heval; Mueller, Marcus; Löwenheim, Hubert; Roccio, Marta; Senn, Pascal

2016-02-01

Objective. Cochlear implants (CIs) have become the gold standard treatment for deafness. These neuroprosthetic devices feature a linear electrode array, surgically inserted into the cochlea, and function by directly stimulating the auditory neurons located within the spiral ganglion, bypassing lost or not-functioning hair cells. Despite their success, some limitations still remain, including poor frequency resolution and high-energy consumption. In both cases, the anatomical gap between the electrode array and the spiral ganglion neurons (SGNs) is believed to be an important limiting factor. The final goal of the study is to characterize response profiles of SGNs growing in intimate contact with an electrode array, in view of designing novel CI devices and stimulation protocols, featuring a gapless interface with auditory neurons. Approach. We have characterized SGN responses to extracellular stimulation using multi-electrode arrays (MEAs). This setup allows, in our view, to optimize in vitro many of the limiting interface aspects between CIs and SGNs. Main results. Early postnatal mouse SGN explants were analyzed after 6-18 days in culture. Different stimulation protocols were compared with the aim to lower the stimulation threshold and the energy needed to elicit a response. In the best case, a four-fold reduction of the energy was obtained by lengthening the biphasic stimulus from 40 μs to 160 μs. Similarly, quasi monophasic pulses were more effective than biphasic pulses and the insertion of an interphase gap moderately improved efficiency. Finally, the stimulation with an external electrode mounted on a micromanipulator showed that the energy needed to elicit a response could be reduced by a factor of five with decreasing its distance from 40 μm to 0 μm from the auditory neurons. Significance. This study is the first to show electrical activity of SGNs on MEAs. Our findings may help to improve stimulation by and to reduce energy consumption of CIs and thereby contribute to the development of fully implantable devices with better auditory resolution in the future.

Selective stimulation of facial muscles with a penetrating electrode array in the feline model

PubMed Central

Sahyouni, Ronald; Bhatt, Jay; Djalilian, Hamid R.; Tang, William C.; Middlebrooks, John C.; Lin, Harrison W.

2017-01-01

Objective Permanent facial nerve injury is a difficult challenge for both patients and physicians given its potential for debilitating functional, cosmetic, and psychological sequelae. Although current surgical interventions have provided considerable advancements in facial nerve rehabilitation, they often fail to fully address all impairments. We aim to introduce an alternative approach to facial nerve rehabilitation. Study design Acute experiments in animals with normal facial function. Methods The study included three anesthetized cats. Four facial muscles (levator auris longus, orbicularis oculi, nasalis, and orbicularis oris) were monitored with a standard electromyographic (EMG) facial nerve monitoring system with needle electrodes. The main trunk of the facial nerve was exposed and a 16-channel penetrating electrode array was placed into the nerve. Electrical current pulses were delivered to each stimulating electrode individually. Elicited EMG voltage outputs were recorded for each muscle. Results Stimulation through individual channels selectively activated restricted nerve populations, resulting in selective contraction of individual muscles. Increasing stimulation current levels resulted in increasing EMG voltage responses. Typically, selective activation of two or more distinct muscles was successfully achieved via a single placement of the multi-channel electrode array by selection of appropriate stimulation channels. Conclusion We have established in the animal model the ability of a penetrating electrode array to selectively stimulate restricted fiber populations within the facial nerve and to selectively elicit contractions in specific muscles and regions of the face. These results show promise for the development of a facial nerve implant system. PMID:27312936

Detuning-induced stimulated Raman adiabatic passage in dense two-level systems

NASA Astrophysics Data System (ADS)

Deng, Li; Lin, Gongwei; Niu, Yueping; Gong, Shangqing

2018-05-01

We investigate the coherence generation in dense two-level systems under detuning-induced stimulated Raman adiabatic passage (D-STIRAP). In the dense two-level system, the near dipole-dipole (NDD) interaction should be taken into consideration. With the increase in the strength of the NDD interaction, it is found that a switchlike transition of the generated coherence from maximum value to zero appears. Meanwhile, the adiabatic condition of the D-STIRAP is destroyed in the presence of the NDD interaction. In order to avoid the sudden decrease in the generated coherence and maintain the maximum value, we can use stronger detuning pulse or pump pulse, between which increasing the intensity of the detuning pulse is of more efficiency. Except for taking advantage of such maximum coherence in the high density case into areas like enhancing the four-wave mixing process, we also point out that the phenomenon of the coherence transition can be applied as an optical switch.

A Low-Cost Energy-Efficient Cableless Geophone Unit for Passive Surface Wave Surveys

PubMed Central

Dai, Kaoshan; Li, Xiaofeng; Lu, Chuan; You, Qingyu; Huang, Zhenhua; Wu, H. Felix

2015-01-01

The passive surface wave survey is a practical, non-invasive seismic exploration method that has increasingly been used in geotechnical engineering. However, in situ deployment of traditional wired geophones is labor intensive for a dense sensor array. Alternatively, stand-alone seismometers can be used, but they are bulky, heavy, and expensive because they are usually designed for long-term monitoring. To better facilitate field applications of the passive surface wave survey, a low-cost energy-efficient geophone system was developed in this study. The hardware design is presented in this paper. To validate the system’s functionality, both laboratory and field experiments were conducted. The unique feature of this newly-developed cableless geophone system allows for rapid field applications of the passive surface wave survey with dense array measurements. PMID:26404270

Optical characterization of nonimaging dish concentrator for the application of dense-array concentrator photovoltaic system.

PubMed

Tan, Ming-Hui; Chong, Kok-Keong; Wong, Chee-Woon

2014-01-20

Optimization of the design of a nonimaging dish concentrator (NIDC) for a dense-array concentrator photovoltaic system is presented. A new algorithm has been developed to determine configuration of facet mirrors in a NIDC. Analytical formulas were derived to analyze the optical performance of a NIDC and then compared with a simulated result obtained from a numerical method. Comprehensive analysis of optical performance via analytical method has been carried out based on facet dimension and focal distance of the concentrator with a total reflective area of 120 m2. The result shows that a facet dimension of 49.8 cm, focal distance of 8 m, and solar concentration ratio of 411.8 suns is the most optimized design for the lowest cost-per-output power, which is US$1.93 per watt.

Internal structure of the San Jacinto fault zone in the trifurcation area southeast of Anza, California, from data of dense seismic arrays

NASA Astrophysics Data System (ADS)

Qin, L.; Ben-Zion, Y.; Qiu, H.; Share, P.-E.; Ross, Z. E.; Vernon, F. L.

2018-04-01

We image the internal structure of the San Jacinto fault zone (SJFZ) in the trifurcation area southeast of Anza, California, with seismic records from dense linear and rectangular arrays. The examined data include recordings from more than 20 000 local earthquakes and nine teleseismic events. Automatic detection algorithms and visual inspection are used to identify P and S body waves, along with P- and S-types fault zone trapped waves (FZTW). The location at depth of the main branch of the SJFZ, the Clark fault, is identified from systematic waveform changes across lines of sensors within the dense rectangular array. Delay times of P arrivals from teleseismic and local events indicate damage asymmetry across the fault, with higher damage to the NE, producing a local reversal of the velocity contrast in the shallow crust with respect to the large-scale structure. A portion of the damage zone between the main fault and a second mapped surface trace to the NE generates P- and S-types FZTW. Inversions of high-quality S-type FZTW indicate that the most likely parameters of the trapping structure are width of ˜70 m, S-wave velocity reduction of 60 per cent, Q value of 60 and depth of ˜2 km. The local reversal of the shallow velocity contrast across the fault with respect to large-scale structure is consistent with preferred propagation of earthquake ruptures in the area to the NW.

Dark Current Reduction of IR Detectors

DTIC Science & Technology

2017-10-19

demonstrating a novel dark current reduction approach for dense infrared detector arrays. This technique is based on the diffusion control junction (DCJ...fabricate and test detector arrays with and without DCJs on the same wafer and demonstrate the effectiveness of the DCJ approach in reducing dark current...subject to any penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number. PLEASE

Plenoptic projection fluorescence tomography.

PubMed

Iglesias, Ignacio; Ripoll, Jorge

2014-09-22

A new method to obtain the three-dimensional localization of fluorochrome distributions in micrometric samples is presented. It uses a microlens array coupled to the image port of a standard microscope to obtain tomographic data by a filtered back-projection algorithm. Scanning of the microlens array is proposed to obtain a dense data set for reconstruction. Simulation and experimental results are shown and the implications of this approach in fast 3D imaging are discussed.

REDUNDANT ARRAY CONFIGURATIONS FOR 21 cm COSMOLOGY

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dillon, Joshua S.; Parsons, Aaron R., E-mail: jsdillon@berkeley.edu

Realizing the potential of 21 cm tomography to statistically probe the intergalactic medium before and during the Epoch of Reionization requires large telescopes and precise control of systematics. Next-generation telescopes are now being designed and built to meet these challenges, drawing lessons from first-generation experiments that showed the benefits of densely packed, highly redundant arrays—in which the same mode on the sky is sampled by many antenna pairs—for achieving high sensitivity, precise calibration, and robust foreground mitigation. In this work, we focus on the Hydrogen Epoch of Reionization Array (HERA) as an interferometer with a dense, redundant core designed followingmore » these lessons to be optimized for 21 cm cosmology. We show how modestly supplementing or modifying a compact design like HERA’s can still deliver high sensitivity while enhancing strategies for calibration and foreground mitigation. In particular, we compare the imaging capability of several array configurations, both instantaneously (to address instrumental and ionospheric effects) and with rotation synthesis (for foreground removal). We also examine the effects that configuration has on calibratability using instantaneous redundancy. We find that improved imaging with sub-aperture sampling via “off-grid” antennas and increased angular resolution via far-flung “outrigger” antennas is possible with a redundantly calibratable array configuration.« less

Redundant Array Configurations for 21 cm Cosmology

NASA Astrophysics Data System (ADS)

Dillon, Joshua S.; Parsons, Aaron R.

2016-08-01

Realizing the potential of 21 cm tomography to statistically probe the intergalactic medium before and during the Epoch of Reionization requires large telescopes and precise control of systematics. Next-generation telescopes are now being designed and built to meet these challenges, drawing lessons from first-generation experiments that showed the benefits of densely packed, highly redundant arrays—in which the same mode on the sky is sampled by many antenna pairs—for achieving high sensitivity, precise calibration, and robust foreground mitigation. In this work, we focus on the Hydrogen Epoch of Reionization Array (HERA) as an interferometer with a dense, redundant core designed following these lessons to be optimized for 21 cm cosmology. We show how modestly supplementing or modifying a compact design like HERA’s can still deliver high sensitivity while enhancing strategies for calibration and foreground mitigation. In particular, we compare the imaging capability of several array configurations, both instantaneously (to address instrumental and ionospheric effects) and with rotation synthesis (for foreground removal). We also examine the effects that configuration has on calibratability using instantaneous redundancy. We find that improved imaging with sub-aperture sampling via “off-grid” antennas and increased angular resolution via far-flung “outrigger” antennas is possible with a redundantly calibratable array configuration.

Pericellular Ca2+ recycling potentiates thrombin-evoked Ca2+ signals in human platelets

PubMed Central

Sage, Stewart O; Pugh, Nicholas; Farndale, Richard W; Harper, Alan G S

2013-01-01

We have previously demonstrated that Na+/Ca2+ exchangers (NCXs) potentiate Ca2+ signaling evoked by thapsigargin in human platelets, via their ability to modulate the secretion of autocoids from dense granules. This link was confirmed in platelets stimulated with the physiological agonist, thrombin, and experiments were performed to examine how Ca2+ removal by the NCX modulates platelet dense granule secretion. In cells loaded with the near-membrane indicator FFP-18, thrombin stimulation was observed to elicit an NCX-dependent accumulation of Ca2+ in a pericellular region around the platelets. To test whether this pericellular Ca2+ accumulation might be responsible for the influence of NCXs over platelet function, platelets were exposed to fast Ca2+ chelators or had their glycocalyx removed. Both manipulations of the pericellular Ca2+ rise reduced thrombin-evoked Ca2+ signals and dense granule secretion. Blocking Ca2+-permeable ion channels had a similar effect, suggesting that Ca2+ exported into the pericellular region is able to recycle back into the platelet cytosol. Single cell imaging with extracellular Fluo-4 indicated that thrombin-evoked rises in extracellular [Ca2+] occurred within the boundary described by the cell surface, suggesting their presence within the open canalicular system (OCS). FFP-18 fluorescence was similarly distributed. These data suggest that upon thrombin stimulation, NCX activity creates a rise in [Ca2+] within the pericellular region of the platelet from where it recycles back into the platelet cytosol, acting to both accelerate dense granule secretion and maintain the initial rise in cytosolic [Ca2+]. PMID:24303163

Electrical stimulus artifact cancellation and neural spike detection on large multi-electrode arrays

PubMed Central

Grosberg, Lauren E.; Madugula, Sasidhar; Litke, Alan; Cunningham, John; Chichilnisky, E. J.; Paninski, Liam

2017-01-01

Simultaneous electrical stimulation and recording using multi-electrode arrays can provide a valuable technique for studying circuit connectivity and engineering neural interfaces. However, interpreting these measurements is challenging because the spike sorting process (identifying and segregating action potentials arising from different neurons) is greatly complicated by electrical stimulation artifacts across the array, which can exhibit complex and nonlinear waveforms, and overlap temporarily with evoked spikes. Here we develop a scalable algorithm based on a structured Gaussian Process model to estimate the artifact and identify evoked spikes. The effectiveness of our methods is demonstrated in both real and simulated 512-electrode recordings in the peripheral primate retina with single-electrode and several types of multi-electrode stimulation. We establish small error rates in the identification of evoked spikes, with a computational complexity that is compatible with real-time data analysis. This technology may be helpful in the design of future high-resolution sensory prostheses based on tailored stimulation (e.g., retinal prostheses), and for closed-loop neural stimulation at a much larger scale than currently possible. PMID:29131818

Electrical stimulus artifact cancellation and neural spike detection on large multi-electrode arrays.

PubMed

Mena, Gonzalo E; Grosberg, Lauren E; Madugula, Sasidhar; Hottowy, Paweł; Litke, Alan; Cunningham, John; Chichilnisky, E J; Paninski, Liam

2017-11-01

Simultaneous electrical stimulation and recording using multi-electrode arrays can provide a valuable technique for studying circuit connectivity and engineering neural interfaces. However, interpreting these measurements is challenging because the spike sorting process (identifying and segregating action potentials arising from different neurons) is greatly complicated by electrical stimulation artifacts across the array, which can exhibit complex and nonlinear waveforms, and overlap temporarily with evoked spikes. Here we develop a scalable algorithm based on a structured Gaussian Process model to estimate the artifact and identify evoked spikes. The effectiveness of our methods is demonstrated in both real and simulated 512-electrode recordings in the peripheral primate retina with single-electrode and several types of multi-electrode stimulation. We establish small error rates in the identification of evoked spikes, with a computational complexity that is compatible with real-time data analysis. This technology may be helpful in the design of future high-resolution sensory prostheses based on tailored stimulation (e.g., retinal prostheses), and for closed-loop neural stimulation at a much larger scale than currently possible.

Imaging San Jacinto Fault damage zone structure using dense linear arrays: application of ambient noise tomography, Rayleigh wave ellipticity, and site amplification

NASA Astrophysics Data System (ADS)

Wang, Y.; Lin, F. C.; Allam, A. A.; Ben-Zion, Y.

2017-12-01

The San Jacinto fault is presently the most seismically active component of the San Andreas Transform system in Southern California. To study the damage zone structure, two dense linear geophone arrays (BS and RR) were deployed across the Clark segment of the San Jacinto Fault between Anza and Hemet during winter 2015 and Fall 2016, respectively. Both arrays were 2 km long with 20 m station spacing. Month-long three-component ambient seismic noise data were recorded and used to calculate multi-channel cross-correlation functions. All three-component noise records of each array were normalized simultaneously to retain relative amplitude information between different stations and different components. We observed clear Rayleigh waves and Love waves on the cross-correlations of both arrays at 0.3 - 1 s period. The phase travel times of the Rayleigh waves on both arrays were measured by frequency-time analysis (FTAN), and inverted for Rayleigh wave phase velocity profiles of the upper 500 m depth. For both arrays, we observe prominent asymmetric low velocity zones which narrow with depth. At the BS array near the Hemet Stepover, an approximately 250m wide slow zone is observed to be offset by 75m to the northeast of the surface fault trace. At the RR array near the Anza segment of the fault, a similar low velocity zone width and offset are observed, along with a 10% across-fault velocity contrast. Analyses of Rayleigh wave ellipticity (H/V ratio), Love wave phase travel times, and site amplification are in progress. By using multiple measurements from ambient noise cross-correlations, we can obtain strong constraints on the local damage zone structure of the San Jacinto Fault. The results contribute to improved understanding of rupture directivity, maximum earthquake magnitude and more generally seismic hazard associated with the San Jacinto fault zone.

Development and validation of a high density SNP genotyping array for Atlantic salmon (Salmo salar).

PubMed

Houston, Ross D; Taggart, John B; Cézard, Timothé; Bekaert, Michaël; Lowe, Natalie R; Downing, Alison; Talbot, Richard; Bishop, Stephen C; Archibald, Alan L; Bron, James E; Penman, David J; Davassi, Alessandro; Brew, Fiona; Tinch, Alan E; Gharbi, Karim; Hamilton, Alastair

2014-02-06

Dense single nucleotide polymorphism (SNP) genotyping arrays provide extensive information on polymorphic variation across the genome of species of interest. Such information can be used in studies of the genetic architecture of quantitative traits and to improve the accuracy of selection in breeding programs. In Atlantic salmon (Salmo salar), these goals are currently hampered by the lack of a high-density SNP genotyping platform. Therefore, the aim of the study was to develop and test a dense Atlantic salmon SNP array. SNP discovery was performed using extensive deep sequencing of Reduced Representation (RR-Seq), Restriction site-Associated DNA (RAD-Seq) and mRNA (RNA-Seq) libraries derived from farmed and wild Atlantic salmon samples (n = 283) resulting in the discovery of > 400 K putative SNPs. An Affymetrix Axiom® myDesign Custom Array was created and tested on samples of animals of wild and farmed origin (n = 96) revealing a total of 132,033 polymorphic SNPs with high call rate, good cluster separation on the array and stable Mendelian inheritance in our sample. At least 38% of these SNPs are from transcribed genomic regions and therefore more likely to include functional variants. Linkage analysis utilising the lack of male recombination in salmonids allowed the mapping of 40,214 SNPs distributed across all 29 pairs of chromosomes, highlighting the extensive genome-wide coverage of the SNPs. An identity-by-state clustering analysis revealed that the array can clearly distinguish between fish of different origins, within and between farmed and wild populations. Finally, Y-chromosome-specific probes included on the array provide an accurate molecular genetic test for sex. This manuscript describes the first high-density SNP genotyping array for Atlantic salmon. This array will be publicly available and is likely to be used as a platform for high-resolution genetics research into traits of evolutionary and economic importance in salmonids and in aquaculture breeding programs via genomic selection.

Development and validation of a high density SNP genotyping array for Atlantic salmon (Salmo salar)

PubMed Central

2014-01-01

Background Dense single nucleotide polymorphism (SNP) genotyping arrays provide extensive information on polymorphic variation across the genome of species of interest. Such information can be used in studies of the genetic architecture of quantitative traits and to improve the accuracy of selection in breeding programs. In Atlantic salmon (Salmo salar), these goals are currently hampered by the lack of a high-density SNP genotyping platform. Therefore, the aim of the study was to develop and test a dense Atlantic salmon SNP array. Results SNP discovery was performed using extensive deep sequencing of Reduced Representation (RR-Seq), Restriction site-Associated DNA (RAD-Seq) and mRNA (RNA-Seq) libraries derived from farmed and wild Atlantic salmon samples (n = 283) resulting in the discovery of > 400 K putative SNPs. An Affymetrix Axiom® myDesign Custom Array was created and tested on samples of animals of wild and farmed origin (n = 96) revealing a total of 132,033 polymorphic SNPs with high call rate, good cluster separation on the array and stable Mendelian inheritance in our sample. At least 38% of these SNPs are from transcribed genomic regions and therefore more likely to include functional variants. Linkage analysis utilising the lack of male recombination in salmonids allowed the mapping of 40,214 SNPs distributed across all 29 pairs of chromosomes, highlighting the extensive genome-wide coverage of the SNPs. An identity-by-state clustering analysis revealed that the array can clearly distinguish between fish of different origins, within and between farmed and wild populations. Finally, Y-chromosome-specific probes included on the array provide an accurate molecular genetic test for sex. Conclusions This manuscript describes the first high-density SNP genotyping array for Atlantic salmon. This array will be publicly available and is likely to be used as a platform for high-resolution genetics research into traits of evolutionary and economic importance in salmonids and in aquaculture breeding programs via genomic selection. PMID:24524230

Validation of in vivo 2D displacements from spiral cine DENSE at 3T.

PubMed

Wehner, Gregory J; Suever, Jonathan D; Haggerty, Christopher M; Jing, Linyuan; Powell, David K; Hamlet, Sean M; Grabau, Jonathan D; Mojsejenko, Walter Dimitri; Zhong, Xiaodong; Epstein, Frederick H; Fornwalt, Brandon K

2015-01-30

Displacement Encoding with Stimulated Echoes (DENSE) encodes displacement into the phase of the magnetic resonance signal. Due to the stimulated echo, the signal is inherently low and fades through the cardiac cycle. To compensate, a spiral acquisition has been used at 1.5T. This spiral sequence has not been validated at 3T, where the increased signal would be valuable, but field inhomogeneities may result in measurement errors. We hypothesized that spiral cine DENSE is valid at 3T and tested this hypothesis by measuring displacement errors at both 1.5T and 3T in vivo. Two-dimensional spiral cine DENSE and tagged imaging of the left ventricle were performed on ten healthy subjects at 3T and six healthy subjects at 1.5T. Intersection points were identified on tagged images near end-systole. Displacements from the DENSE images were used to project those points back to their origins. The deviation from a perfect grid was used as a measure of accuracy and quantified as root-mean-squared error. This measure was compared between 3T and 1.5T with the Wilcoxon rank sum test. Inter-observer variability of strains and torsion quantified by DENSE and agreement between DENSE and harmonic phase (HARP) were assessed by Bland-Altman analyses. The signal to noise ratio (SNR) at each cardiac phase was compared between 3T and 1.5T with the Wilcoxon rank sum test. The displacement accuracy of spiral cine DENSE was not different between 3T and 1.5T (1.2 ± 0.3 mm and 1.2 ± 0.4 mm, respectively). Both values were lower than the DENSE pixel spacing of 2.8 mm. There were no substantial differences in inter-observer variability of DENSE or agreement of DENSE and HARP between 3T and 1.5T. Relative to 1.5T, the SNR at 3T was greater by a factor of 1.4 ± 0.3. The spiral cine DENSE acquisition that has been used at 1.5T to measure cardiac displacements can be applied at 3T with equivalent accuracy. The inter-observer variability and agreement of DENSE-derived peak strains and torsion with HARP is also comparable at both field strengths. Future studies with spiral cine DENSE may take advantage of the additional SNR at 3T.

Photovoltaic restoration of sight with high visual acuity

PubMed Central

Lorach, Henri; Goetz, Georges; Smith, Richard; Lei, Xin; Mandel, Yossi; Kamins, Theodore; Mathieson, Keith; Huie, Philip; Harris, James; Sher, Alexander; Palanker, Daniel

2015-01-01

Patients with retinal degeneration lose sight due to gradual demise of photoreceptors. Electrical stimulation of the surviving retinal neurons provides an alternative route for delivery of visual information. We demonstrate that subretinal arrays with 70 μm photovoltaic pixels provide highly localized stimulation, with electrical and visual receptive fields of comparable sizes in rat retinal ganglion cells. Similarly to normal vision, retinal response to prosthetic stimulation exhibits flicker fusion at high frequencies, adaptation to static images and non-linear spatial summation. In rats with retinal degeneration, these photovoltaic arrays provide spatial resolution of 64 ± 11 μm, corresponding to half of the normal visual acuity in pigmented rats. Ease of implantation of these wireless and modular arrays, combined with their high resolution opens the door to functional restoration of sight. PMID:25915832

Performance of photovoltaic arrays in-vivo and characteristics of prosthetic vision in animals with retinal degeneration

PubMed Central

Lorach, Henri; Goetz, Georges; Mandel, Yossi; Lei, Xin; Kamins, Theodore I.; Mathieson, Keith; Huie, Philip; Dalal, Roopa; Harris, James S.; Palanker, Daniel

2014-01-01

Summary Loss of photoreceptors during retinal degeneration leads to blindness, but information can be reintroduced into the visual system using electrical stimulation of the remaining retinal neurons. Subretinal photovoltaic arrays convert pulsed illumination into pulsed electric current to stimulate the inner retinal neurons. Since required irradiance exceeds the natural luminance levels, an invisible near-infrared (915nm) light is used to avoid photophobic effects. We characterized the thresholds and dynamic range of cortical responses to prosthetic stimulation with arrays of various pixel sizes and with different number of photodiodes. Stimulation thresholds for devices with 140µm pixels were approximately half those of 70µm pixels, and with both pixel sizes, thresholds were lower with 2 diodes than with 3 diodes per pixel. In all cases these thresholds were more than two orders of magnitude below the ocular safety limit. At high stimulation frequencies (>20Hz), the cortical response exhibited flicker fusion. Over one order of magnitude of dynamic range could be achieved by varying either pulse duration or irradiance. However, contrast sensitivity was very limited. Cortical responses could be detected even with only a few illuminated pixels. Finally, we demonstrate that recording of the corneal electric potential in response to patterned illumination of the subretinal arrays allows monitoring the current produced by each pixel, and thereby assessing the changes in the implant performance over time. PMID:25255990

Construction and application of a bovine immune-endocrine cDNA microarray.

PubMed

Tao, Wenjing; Mallard, Bonnie; Karrow, Niel; Bridle, Byram

2004-09-01

A variety of commercial DNA arrays specific for humans and rodents are widely available; however, microarrays containing well-characterized genes to study pathway-specific gene expression are not as accessible for domestic animals, such as cattle, sheep and pigs. Therefore, a small-scale application-targeted bovine immune-endocrine cDNA array was developed to evaluate genetic pathways involved in the immune-endocrine axis of cattle during periods of altered homeostasis provoked by physiological or environmental stressors, such as infection, vaccination or disease. For this purpose, 167 cDNA sequences corresponding to immune, endocrine and inflammatory response genes were collected and categorized. Positive controls included 5 housekeeping genes (glyceraldehydes-3-phosphate dehydrogenase, hypoxanthine phosphoribosyltransferase, ribosomal protein L19, beta-actin, beta2-microglobulin) and bovine genomic DNA. Negative controls were a bacterial gene (Rhodococcus equi 17-kDa virulence-associated protein) and a partial sequence of the plasmid pACYC177. In addition, RNA extracted from un-stimulated, as well as superantigen (Staphylococcus aureus enterotoxin-A, S. aureus Cowan Pansorbin Cells) and mitogen-stimulated (LPS, ConA) bovine blood leukocytes was mixed, reverse transcribed and PCR amplified using gene-specific primers. The endocrine-associated genes were amplified from cDNA derived from un-stimulated bovine hypothalamus, pituitary, adrenal and thyroid gland tissues. The array was constructed in 4 repeating grids of 180 duplicated spots by coupling the PCR amplified 213-630 bp gene fragments onto poly-l-lysine coated glass slides. The bovine immune-endocrine arrays were standardized and preliminary gene expression profiles generated using Cy3 and Cy5 labelled cDNA from un-stimulated and ConA (5 microg/ml) stimulated PBMC of 4 healthy Holstein cows (2-4 replicate arrays/cow) in a time course study. Mononuclear cell-derived cytokine and chemokine (IL-2, IL-1alpha, TNFalpha, IFN-gamma, TGFbeta-1, MCP-1, MCP-2 and MIP-3alpha) mRNA exhibited a repeatable and consistently low expression in un-stimulated cells and at least a two-fold increased expression following 6 and 24 h ConA stimulation as compared to 0 h un-stimulated controls. In contrast, expression of antigen presenting molecules, MHC-DR, MHC-DQ and MHC-DY, were consistently at least two-fold lower following 6 and 24 h ConA stimulation. The only endocrine gene with differential expression following ConA stimulation was prolactin. Additionally, due to the high level of genetic homology between ovine, swine and bovine genes, RNA similarly acquired from sheep and pigs was evaluated and similar gene expression patterns were noted. These data demonstrate that this application-targeted array containing a set of well characterized genes can be used to determine the relative gene expression corresponding to immune-endocrine responses of cattle and related species, sheep and pigs.

Advances in selective activation of muscles for non-invasive motor neuroprostheses.

PubMed

Koutsou, Aikaterini D; Moreno, Juan C; Del Ama, Antonio J; Rocon, Eduardo; Pons, José L

2016-06-13

Non-invasive neuroprosthetic (NP) technologies for movement compensation and rehabilitation remain with challenges for their clinical application. Two of those major challenges are selective activation of muscles and fatigue management. This review discusses how electrode arrays improve the efficiency and selectivity of functional electrical stimulation (FES) applied via transcutaneous electrodes. In this paper we review the principles and achievements during the last decade on techniques for artificial motor unit recruitment to improve the selective activation of muscles. We review the key factors affecting the outcome of muscle force production via multi-pad transcutaneous electrical stimulation and discuss how stimulation parameters can be set to optimize external activation of body segments. A detailed review of existing electrode array systems proposed by different research teams is also provided. Furthermore, a review of the targeted applications of existing electrode arrays for control of upper and lower limb NPs is provided. Eventually, last section demonstrates the potential of electrode arrays to overcome the major challenges of NPs for compensation and rehabilitation of patient-specific impairments.

Demonstration of a setup for chronic optogenetic stimulation and recording across cortical areas in non-human primates

NASA Astrophysics Data System (ADS)

Yazdan-Shahmorad, Azadeh; Diaz-Botia, Camilo; Hanson, Tim; Ledochowitsch, Peter; Maharabiz, Michel M.; Sabes, Philip N.

2015-03-01

Although several studies have shown the feasibility of using optogenetics in non-human primates (NHP), reliable largescale chronic interfaces have not yet been reported for such studies in NHP. Here we introduce a chronic setup that permits repeated, daily optogenetic stimulation and large-scale recording from the same sites in NHP cortex. The setup combines optogenetics with a transparent artificial dura (AD) and high-density micro-electrocorticography (μECoG). To obtain expression across large areas of cortex, we infused AAV5-CamKIIa-C1V1-EYFP viral vector using an infusion technique based on convection-enhanced delivery (CED) in primary somatosensory (S1) and motor (M1) cortices. By epifluorescent imaging through AD we were able to confirm high levels of expression covering about 110 mm2 of S1 and M1. We then incorporated a 192-channel μECoG array spanning 192 mm2 into the AD for simultaneous electrophysiological recording during optical stimulation. The array consists of patterned Pt-Au-Pt metal traces embedded in ~10 μm Parylene-C insulator. The parylene is sufficiently transparent to allow minimally attenuated optical access for optogenetic stimulation. The array was chronically implanted over the opsin-expressing areas in M1 and S1 for over two weeks. Optical stimulation was delivered via a fiber optic placed on the surface of the AD. With this setup, we recorded reliable evoked activity following light stimulation at several locations. Similar responses were recorded across tens of days, however a decline in the light-evoked signal amplitude was observed during this period due to the growth of dural tissue over the array. These results show the feasibility of a chronic interface for combined largescale optogenetic stimulation and cortical recordings across days.

[Finite element analysis of temperature field of retina by electrical stimulation with microelectrode array].

PubMed

Wang, Wei; Qiao, Qingli; Gao, Weiping; Wu, Jun

2014-12-01

We studied the influence of electrode array parameters on temperature distribution to the retina during the use of retinal prosthesis in order to avoid thermal damage to retina caused by long-term electrical stimulation. Based on real epiretinal prosthesis, a three-dimensional model of electrical stimulation for retina with 4 X 4 microelectrode array had been established using the finite element software (COMSOL Multiphysics). The steady-state temperature field of electrical stimulation of the retina was calculated, and the effects of the electrode parameters such as the distance between the electrode contacts, the materials and area of the electrode contact on temperature field were considered. The maximum increase in the retina steady temperature was about 0. 004 degrees C with practical stimulation current. When the distance between the electrode contacts was changed from 130 microm to 520 microm, the temperature was reduced by about 0.006 microC. When the contact radius was doubled from 130 microm to 260 microm, the temperature decrease was about 0.005 degrees C. It was shown that there were little temperature changes in the retina with a 4 x 4 epiretinal microelectrode array, reflecting the safety of electrical stimulation. It was also shown that the maximum temperature in the retina decreased with increasing the distance between the electrode contacts, as well as increasing the area of electrode contact. However, the change of the maximum temperature was very small when the distance became larger than the diameter of electrode contact. There was no significant difference in the effects of temperature increase among the different electrode materials. Rational selection of the distance between the electrode contacts and their area in electrode design can reduce the temperature rise induced by electrical stimulation.

Copper-encapsulated vertically aligned carbon nanotube arrays.

PubMed

Stano, Kelly L; Chapla, Rachel; Carroll, Murphy; Nowak, Joshua; McCord, Marian; Bradford, Philip D

2013-11-13

A new procedure is described for the fabrication of vertically aligned carbon nanotubes (VACNTs) that are decorated, and even completely encapsulated, by a dense network of copper nanoparticles. The process involves the conformal deposition of pyrolytic carbon (Py-C) to stabilize the aligned carbon-nanotube structure during processing. The stabilized arrays are mildly functionalized using oxygen plasma treatment to improve wettability, and they are then infiltrated with an aqueous, supersaturated Cu salt solution. Once dried, the salt forms a stabilizing crystal network throughout the array. After calcination and H2 reduction, Cu nanoparticles are left decorating the CNT surfaces. Studies were carried out to determine the optimal processing parameters to maximize Cu content in the composite. These included the duration of Py-C deposition and system process pressure as well as the implementation of subsequent and multiple Cu salt solution infiltrations. The optimized procedure yielded a nanoscale hybrid material where the anisotropic alignment from the VACNT array was preserved, and the mass of the stabilized arrays was increased by over 24-fold because of the addition of Cu. The procedure has been adapted for other Cu salts and can also be used for other metal salts altogether, including Ni, Co, Fe, and Ag. The resulting composite is ideally suited for application in thermal management devices because of its low density, mechanical integrity, and potentially high thermal conductivity. Additionally, further processing of the material via pressing and sintering can yield consolidated, dense bulk composites.

Seismic Imaging of a Prospective Geothermal Play, Using a Dense Geophone Array

NASA Astrophysics Data System (ADS)

Trow, A.; Pankow, K. L.; Wannamaker, P. E.; Lin, F. C.; Ward, K. M.

2017-12-01

In the summer of 2016 a dense array of 48 Nodal Seismic geophones was deployed near Beaver, Utah on the eastern flank of the Mineral Mountains. The array aperture was approximately 20 kilometers and recorded continuous seismic data for 30 days. Geophones were centered on a previously known shallow (5km depth) magnetolluric (MT) low-resistivity body. This region of low resistivity was interpreted to possibly contain hydrothermal/geothermal fluids and was targeted for further seismic investigation. The seismic array geometry was designed to optimize seismic event detection for small (magnitude of completeness zero) earthquakes and to facilitate seismic imaging at depths of 5 km and deeper. For the duration of the experiment, one ML 1 earthquake was detected underneath the array with 15 other earthquakes detected to the east and south in the more seismically active Pavant Range. Different passive imaging techniques, including ambient noise and earthquake tomography are being explored in order to produce a seismic velocity image. Understanding the subsurface, specifically the fracture network and fluid content of the bedrock is important for characterization of a geothermal prospect. If it is rich in fluids, it can be assumed that some fracture network is in place to accommodate such fluids. Both fractures and fluid content of the prospect will have an effect on the seismic velocities in the basement structure. These properties can help determine the viability of a geothermal system for power production.

A New Individually Addressable Micro-LED Array for Photogenetic Neural Stimulation.

PubMed

McGovern, B; Berlinguer Palmini, R; Grossman, N; Drakakis, E; Poher, V; Neil, M A A; Degenaar, P

2010-12-01

Here, we demonstrate the use of a micro light emitting diode (LED) array as a powerful tool for complex spatiotemporal control of photosensitized neurons. The array can generate arbitrary, 2-D, excitation patterns with millisecond and micrometer resolution. In particular, we describe an active matrix control address system to allow simultaneous control of 256 individual micro LEDs. We present the system optically integrated into a microscope environment and patch clamp electrophysiology. The results show that the emitters have sufficient radiance at the required wavelength to stimulate neurons expressing channelrhodopsin-2 (ChR2).

CMZoom: The Submillimeter Array Survey of our Galaxy’s Central Molecular Zone

NASA Astrophysics Data System (ADS)

Battersby, Cara; CMZoom Team

2018-01-01

The inner few hundred parsecs of the Milky Way, the Central Molecular Zone (CMZ), is our closest laboratory for understanding star formation in the extreme environments (hot, dense, turbulent gas) that once dominated the universe. We present an update on the first large-area survey to expose the sites of star formation across the CMZ at high-resolution in submillimeter wavelengths: the CMZoom survey with the Submillimeter Array (SMA). We identify the locations of dense cores and search for signatures of embedded star formation. CMZoom is a three-year survey, completed this year, and has mapped out the highest column density regions of the CMZ in dust continuum and a variety of spectral lines around 1.3 mm. CMZoom combines SMA compact and subcompact configurations with single-dish data from BGPS and the APEX telescope, achieving an angular resolution of about 4” (0.2 pc) and good image fidelity up to large spatial scales.

Muscle Stimulation Technology

NASA Technical Reports Server (NTRS)

1997-01-01

Under a Goddard Space Flight Center contract, Electrologic of America was able to refine the process of densely packing circuitry on personal computer boards, providing significant contributions to the closed-loop systems for the Remote Manipulator System Simulator. The microcircuitry work was then applied to the StimMaster FES Ergometer, an exercise device used to stimulate muscles suffering from paralysis. The electrical stimulation equipment was developed exclusively for V-Care Health Systems, Inc. Product still commercially available as of March 2002.

Association of infertility and fertility treatment with mammographic density in a large screening-based cohort of women: a cross-sectional study.

PubMed

Lundberg, Frida E; Johansson, Anna L V; Rodriguez-Wallberg, Kenny; Brand, Judith S; Czene, Kamila; Hall, Per; Iliadou, Anastasia N

2016-04-13

Ovarian stimulation drugs, in particular hormonal agents used for controlled ovarian stimulation (COS) required to perform in vitro fertilization, increase estrogen and progesterone levels and have therefore been suspected to influence breast cancer risk. This study aims to investigate whether infertility and hormonal fertility treatment influences mammographic density, a strong hormone-responsive risk factor for breast cancer. Cross-sectional study including 43,313 women recruited to the Karolinska Mammography Project between 2010 and 2013. Among women who reported having had infertility, 1576 had gone through COS, 1429 had had hormonal stimulation without COS and 5958 had not received any hormonal fertility treatment. Percent and absolute mammographic densities were obtained using the volumetric method Volpara™. Associations with mammographic density were assessed using multivariable generalized linear models, estimating mean differences (MD) with 95 % confidence intervals (CI). After multivariable adjustment, women with a history of infertility had 1.53 cm(3) higher absolute dense volume compared to non-infertile women (95 % CI: 0.70 to 2.35). Among infertile women, only those who had gone through COS treatment had a higher absolute dense volume than those who had not received any hormone treatment (adjusted MD 3.22, 95 % CI: 1.10 to 5.33). No clear associations were observed between infertility, fertility treatment and percent volumetric density. Overall, women reporting infertility had more dense tissue in the breast. The higher absolute dense volume in women treated with COS may indicate a treatment effect, although part of the association might also be due to the underlying infertility. Continued monitoring of cancer risk in infertile women, especially those who undergo COS, is warranted.

Modeling Intracochlear Magnetic Stimulation: A Finite-Element Analysis.

PubMed

Mukesh, S; Blake, D T; McKinnon, B J; Bhatti, P T

2017-08-01

This study models induced electric fields, and their gradient, produced by pulsatile current stimulation of submillimeter inductors for cochlear implantation. Using finite-element analysis, the lower chamber of the cochlea, scala tympani, is modeled as a cylindrical structure filled with perilymph bounded by tissue, bone, and cochlear neural elements. Single inductors as well as an array of inductors are modeled. The coil strength (~100 nH) and excitation parameters (peak current of 1-5 A, voltages of 16-20 V) are based on a formative feasibility study conducted by our group. In that study, intracochlear micromagnetic stimulation achieved auditory activation as measured through the auditory brainstem response in a feline model. With respect to the finite element simulations, axial symmetry of the inductor geometry is exploited to improve computation time. It is verified that the inductor coil orientation greatly affects the strength of the induced electric field and thereby the ability to affect the transmembrane potential of nearby neural elements. Furthermore, upon comparing an array of micro-inductors with a typical multi-site electrode array, magnetically excited arrays retain greater focus in terms of the gradient of induced electric fields. Once combined with further in vivo analysis, this modeling study may enable further exploration of the mechanism of magnetically induced, and focused neural stimulation.

Virtual source reflection imaging of the Socorro Magma Body, New Mexico, using a dense seismic array

NASA Astrophysics Data System (ADS)

Finlay, T. S.; Worthington, L. L.; Schmandt, B.; Hansen, S. M.; Bilek, S. L.; Aster, R. C.; Ranasinghe, N. R.

2017-12-01

The Socorro Magma Body (SMB) is one of the largest known actively inflating continental magmatic intrusions. Previous studies have relied on sparse instrument coverage to determine its spatial extent, depth, and seismic signature, which characterized the body as a thin sill with a surface at 19 km below the Earth's surface. However, over the last two decades, InSAR and magneto-telluric (MT) studies have shed new light on the SMB and invigorated the scientific debate of the spatial distribution and uplift rate of the SMB. We return to seismic imaging of the SMB with the Sevilleta Array, a 12-day deployment of approximately 800 vertical component, 10-Hz geophones north of Socorro, New Mexico above and around the estimated northern half of the SMB. Teleseismic virtual source reflection profiling (TVR) employs the free surface reflection off of a teleseismic P as a virtual source in dense arrays, and has been used successfully to image basin structure and the Moho in multiple tectonic environments. The Sevilleta Array recorded 62 teleseismic events greater than M5. Applying TVR to the data collected by the Sevilleta Array, we present stacks from four events that produced the with high signal-to-noise ratios and simple source-time functions: the February 11, 2015 M6.7 in northern Argentina, the February 19, 2015 M5.4 in Kamchatka, Russia, and the February 21, 2015 M5.1 and February 22, 2015 M5.5 in western Colombia. Preliminary results suggest eastward-dipping reflectors at approximately 5 km depth near the Sierra Ladrones range in the northwestern corner of the array. Further analysis will focus on creating profiles across the area of maximum SMB uplift and constraining basin geometry.

Hemispherical array of sensors with contractively wrapped polymer petals for flow sensing

NASA Astrophysics Data System (ADS)

Kanhere, Elgar; Wang, Nan; Kottapalli, Ajay Giri Prakash; Miao, Jianmin; Triantafyllou, Michael

2017-11-01

Hemispherical arrays have inherent advantages that allow simultaneous detection of flow speed and direction due to their shape. Though MEMS technology has progressed leaps and bounds, fabrication of array of sensors on a hemispherical surface is still a challenge. In this work, a novel approach of constructing hemispherical array is presented which employs a technique of contractively wrapping a hemispherical surface with flexible liquid crystal polymer petals. This approach also leverages the offerings from rapid prototyping technology and established standard MEMS fabrication processes. Hemispherical arrays of piezoresistive sensors are constructed with two types of petal wrappings, 4-petals and 8-petals, on a dome. The flow sensing and direction detection abilities of the dome are evaluated through experiments in wind tunnel. Experimental results demonstrate that a dome equipped with a dense array of sensors can provide information pertaining to the stimulus, through visualization of output profile over the entire surface.

Development of optics with micro-LED arrays for improved opto-electronic neural stimulation

NASA Astrophysics Data System (ADS)

Chaudet, Lionel; Neil, Mark; Degenaar, Patrick; Mehran, Kamyar; Berlinguer-Palmini, Rolando; Corbet, Brian; Maaskant, Pleun; Rogerson, David; Lanigan, Peter; Bamberg, Ernst; Roska, Botond

2013-03-01

The breakthrough discovery of a nanoscale optically gated ion channel protein, Channelrhodopsin 2 (ChR2), and its combination with a genetically expressed ion pump, Halorhodopsin, allowed the direct stimulation and inhibition of individual action potentials with light alone. This work reports developments of ultra-bright elec­ tronically controlled optical array sources with enhanced light gated ion channels and pumps for use in systems to further our understanding of both brain and visual function. This work is undertaken as part of the European project, OptoNeuro. Micro-LED arrays permit spatio-temporal control of neuron stimulation on sub-millisecond timescales. However they are disadvantaged by their broad spatial light emission distribution and low fill factor. We present the design and implementation of a projection and micro-optics system for use with a micro-LED array consisting of a 16x16 matrix of 25 μm diameter micro-LEDs with 150 μm centre-to-centre spacing and an emission spectrum centred at 470 nm overlapping the peak sensitivity of ChR2 and its testing on biological samples. The projection system images the micro-LED array onto micro-optics to improve the fill-factor from ~2% to more than 78% by capturing a larger fraction of the LED emission and directing it correctly to the sample plane. This approach allows low fill factor arrays to be used effectively, which in turn has benefits in terms of thermal management and electrical drive from CMOS backplane electronics. The entire projection system is integrated into a microscope prototype to provide stimulation spots at the same size as the neuron cell body (μ10 pm).

The Green Canyon Event as Recorded by the Atlantis OBS Node Survey

NASA Astrophysics Data System (ADS)

Dellinger, J. A.; Ehlers, J.; Clarke, R.

2006-12-01

On 10 February, 2006, a magnitude 5.2 earthquake occurred 260~km South of New Orleans, Louisiana, in the Green Canyon area of the United States Gulf of Mexico. Fortuitously, at the time of the earthquake an array of nearly 500 ocean-bottom-seismic nodes happened to be recording about 40~km SouthEast of the epicenter. These nodes were part of an ongoing oil-exploration 3D-seismic survey ("Atlantis patch 2"), and were designed to record oil-exploration air-gun seismic signals (with a dominant frequency of about 15~Hz), not low-frequency earthquake signals (1~Hz). The survey's own air guns, located about 7~km to the SouthEast of the array at the time of the event, were also repeatedly firing, generating large amounts of "noise" (at least for the purposes of analyzing the earthquake signal). Not surprisingly, when the data are plotted at their original sample rate they are dominated by the Atlantis survey's air-gun signal. When low passed with an upper cutoff of 2~Hz, however, the air-gun signals essentially vanish and underlying natural signals are clearly revealed. In land-seismic exploration dense 3D arrays of single geophones are used to characterize unwanted surface-wave energy. Beam forming the dense array allows the directions and phase velocities of wavefronts propagating across the array to be identified and localized so that receiver arrays can be designed that best attenuate the surface-wave noise. The 400-meter spacing of the Atlantis node array was designed to be optimally sparse for reflection-seismic processing. At 1~Hz, however, a 400-meter spacing becomes "dense" and we were able to use the same toolkit of programs originally developed for analyzing surface waves in land-seismic data to analyze the earthquake waves. The analysis reveals a complex and protracted series of arrivals spanning nearly 20~minutes of time. The expected sequence of earthquake arrivals from the North-NorthWest are followed by weaker sequences of arrivals of unknown origin from first the SouthEast and then from the East. It is hoped that these data can be used to help constrain the location, depth, and mechanism of the Green Canyon event. The authors wish to thank BP and BHPB for their permission to present this work, Fairfield for their enthusiasm in preserving the data, and CGG, WesternGeco, and Fugro for their cooperation in identifying other sources of man-made signals in the data.

A Comparison of Earthquake Back-Projection Imaging Methods for Dense Local Arrays, and Application to the 2011 Virginia Aftershock Sequence

NASA Astrophysics Data System (ADS)

Beskardes, G. D.; Hole, J. A.; Wang, K.; Wu, Q.; Chapman, M. C.; Davenport, K. K.; Michaelides, M.; Brown, L. D.; Quiros, D. A.

2016-12-01

Back-projection imaging has recently become a practical method for local earthquake detection and location due to the deployment of densely sampled, continuously recorded, local seismograph arrays. Back-projection is scalable to earthquakes with a wide range of magnitudes from very tiny to very large. Local dense arrays provide the opportunity to capture very tiny events for a range applications, such as tectonic microseismicity, source scaling studies, wastewater injection-induced seismicity, hydraulic fracturing, CO2 injection monitoring, volcano studies, and mining safety. While back-projection sometimes utilizes the full seismic waveform, the waveforms are often pre-processed to overcome imaging issues. We compare the performance of back-projection using four previously used data pre-processing methods: full waveform, envelope, short-term averaging / long-term averaging (STA/LTA), and kurtosis. The goal is to identify an optimized strategy for an entirely automated imaging process that is robust in the presence of real-data issues, has the lowest signal-to-noise thresholds for detection and for location, has the best spatial resolution of the energy imaged at the source, preserves magnitude information, and considers computational cost. Real data issues include aliased station spacing, low signal-to-noise ratio (to <1), large noise bursts and spatially varying waveform polarity. For evaluation, the four imaging methods were applied to the aftershock sequence of the 2011 Virginia earthquake as recorded by the AIDA array with 200-400 m station spacing. These data include earthquake magnitudes from -2 to 3 with highly variable signal to noise, spatially aliased noise, and large noise bursts: realistic issues in many environments. Each of the four back-projection methods has advantages and disadvantages, and a combined multi-pass method achieves the best of all criteria. Preliminary imaging results from the 2011 Virginia dataset will be presented.

A comparison of earthquake backprojection imaging methods for dense local arrays

NASA Astrophysics Data System (ADS)

Beskardes, G. D.; Hole, J. A.; Wang, K.; Michaelides, M.; Wu, Q.; Chapman, M. C.; Davenport, K. K.; Brown, L. D.; Quiros, D. A.

2018-03-01

Backprojection imaging has recently become a practical method for local earthquake detection and location due to the deployment of densely sampled, continuously recorded, local seismograph arrays. While backprojection sometimes utilizes the full seismic waveform, the waveforms are often pre-processed and simplified to overcome imaging challenges. Real data issues include aliased station spacing, inadequate array aperture, inaccurate velocity model, low signal-to-noise ratio, large noise bursts and varying waveform polarity. We compare the performance of backprojection with four previously used data pre-processing methods: raw waveform, envelope, short-term averaging/long-term averaging and kurtosis. Our primary goal is to detect and locate events smaller than noise by stacking prior to detection to improve the signal-to-noise ratio. The objective is to identify an optimized strategy for automated imaging that is robust in the presence of real-data issues, has the lowest signal-to-noise thresholds for detection and for location, has the best spatial resolution of the source images, preserves magnitude, and considers computational cost. Imaging method performance is assessed using a real aftershock data set recorded by the dense AIDA array following the 2011 Virginia earthquake. Our comparisons show that raw-waveform backprojection provides the best spatial resolution, preserves magnitude and boosts signal to detect events smaller than noise, but is most sensitive to velocity error, polarity error and noise bursts. On the other hand, the other methods avoid polarity error and reduce sensitivity to velocity error, but sacrifice spatial resolution and cannot effectively reduce noise by stacking. Of these, only kurtosis is insensitive to large noise bursts while being as efficient as the raw-waveform method to lower the detection threshold; however, it does not preserve the magnitude information. For automatic detection and location of events in a large data set, we therefore recommend backprojecting kurtosis waveforms, followed by a second pass on the detected events using noise-filtered raw waveforms to achieve the best of all criteria.

Structure of the North Anatolian Fault Zone from the Auto-Correlation of Ambient Seismic Noise Recorded at a Dense Seismometer Array

NASA Astrophysics Data System (ADS)

Taylor, D. G.; Rost, S.; Houseman, G.

2015-12-01

In recent years the technique of cross-correlating the ambient seismic noise wavefield at two seismometers to reconstruct empirical Green's Functions for the determination of Earth structure has been a powerful tool to study the Earth's interior without earthquake or man-made sources. However, far less attention has been paid to using auto-correlations of seismic noise to reveal body wave reflections from interfaces in the subsurface. In principle, the Green's functions thus derived should be comparable to the Earth's impulse response to a co-located source and receiver. We use data from a dense seismic array (Dense Array for Northern Anatolia - DANA) deployed across the northern branch of the North Anatolian Fault Zone (NAFZ) in the region of the 1999 magnitude 7.6 Izmit earthquake in western Turkey. The NAFZ is a major strike-slip system that extends ~1200 km across northern Turkey and continues to pose a high level of seismic hazard, in particular to the mega-city of Istanbul. We construct reflection images for the entire crust and upper mantle over the ~35 km by 70 km footprint of the 70-station DANA array. Using auto-correlations of vertical and horizontal components of ground motion, both P- and S-wave velocity information can be retrieved from the wavefield to constrain crustal structure further to established methods. We show that clear P-wave reflections from the crust-mantle boundary (Moho) can be retrieved using the autocorrelation technique, indicating topography on the Moho on horizontal scales of less than 10 km. Offsets in crustal structure can be identified that seem to be correlated with the surface expression of the fault zone in the region. The combined analysis of auto-correlations using vertical and horizontal components will lead to further insight into the fault zone structure throughout the crust and upper mantle.

Highly Flexible Silicone Coated Neural Array for Intracochlear Electrical Stimulation

PubMed Central

Bhatti, P.; Van Beek-King, J.; Sharpe, A.; Crawford, J.; Tridandapani, S.; McKinnon, B.; Blake, D.

2015-01-01

We present an effective method for tailoring the flexibility of a commercial thin-film polymer electrode array for intracochlear electrical stimulation. Using a pneumatically driven dispensing system, an average 232 ± 64 μm (mean ± SD) thickness layer of silicone adhesive coating was applied to stiffen the underside of polyimide multisite arrays. Additional silicone was applied to the tip to protect neural tissue during insertion and along the array to improve surgical handling. Each array supported 20 platinum sites (180 μm dia., 250 μm pitch), spanning nearly 28 mm in length and 400 μm in width. We report an average intracochlear stimulating current threshold of 170 ± 93 μA to evoke an auditory brainstem response in 7 acutely deafened felines. A total of 10 arrays were each inserted through a round window approach into the cochlea's basal turn of eight felines with one delamination occurring upon insertion (preliminary results of the in vivo data presented at the 48th Annual Meeting American Neurotology Society, Orlando, FL, April 2013, and reported in Van Beek-King 2014). Using microcomputed tomography imaging (50 μm resolution), distances ranging from 100 to 565 μm from the cochlea's central modiolus were measured. Our method combines the utility of readily available commercial devices with a straightforward postprocessing step on the order of 24 hours. PMID:26236714

Electrochemical properties of Ti3+ doped Ag-Ti nanotube arrays coated with hydroxyapatite

NASA Astrophysics Data System (ADS)

Zhang, Hangzhou; Shi, Xiaoguo; Tian, Ang; Wang, Li; Liu, Chuangwei

2018-04-01

Ag-Ti nanotube array was prepared by simple anodic oxidation method and uniform hydroxyapatite were electrochemically deposited on the nanotubes, and then characterized by SEM, XRD, XPS and EIS. In order to investigate the influence of Ti3+ on the electrochemical deposition of hydroxyapatite on the nanotubes, the Ag-Ti nanotube array self-doped with Ti3+ was prepared by one step reduction method. The experiment results revealed that the Ti3+ can promote the grow rate of hydroxyapatite coatings on nanotube surface. The hydroxyapatite coated Ag-Ti nanotube arrays with Ti3+ exhibit excellent stability and higher corrosion resistance. Moreover, the compact and dense hydroxyapatite coating can also prevent the Ag atom erosion from the Ag-Ti nanotube.

Magnetic Calorimeter Arrays with High Sensor Inductance and Dense Wiring

NASA Astrophysics Data System (ADS)

Stevenson, T. R.; Balvin, M. A.; Bandler, S. R.; Devasia, A. M.; Nagler, P. C.; Smith, S. J.; Yoon, W.

2018-05-01

We describe prototype arrays of magnetically coupled microcalorimeters fabricated with an approach scalable to very large format arrays. The superconducting interconnections and sensor coils have sufficiently low inductance in the wiring and sufficiently high inductance in the coils in each pixel, to enable arrays containing greater than 4000 sensors and 100,000 X-ray absorbers to be used in future astrophysics missions such as Lynx. We have used projection lithography to create submicron patterns (e.g., 400 nm lines and spaces) in our niobium sensor coils and wiring, integrated with gold-erbium sensor films and gold X-ray absorbers. Our prototype devices will explore the device physics of metallic magnetic calorimeters as feature sizes are reduced to nanoscale.

Crowding-facilitated macromolecular transport in attractive micropost arrays.

PubMed

Chien, Fan-Tso; Lin, Po-Keng; Chien, Wei; Hung, Cheng-Hsiang; Yu, Ming-Hung; Chou, Chia-Fu; Chen, Yeng-Long

2017-05-02

Our study of DNA dynamics in weakly attractive nanofabricated post arrays revealed crowding enhances polymer transport, contrary to hindered transport in repulsive medium. The coupling of DNA diffusion and adsorption to the microposts results in more frequent cross-post hopping and increased long-term diffusivity with increased crowding density. We performed Langevin dynamics simulations and found maximum long-term diffusivity in post arrays with gap sizes comparable to the polymer radius of gyration. We found that macromolecular transport in weakly attractive post arrays is faster than in non-attractive dense medium. Furthermore, we employed hidden Markov analysis to determine the transition of macromolecular adsorption-desorption on posts and hopping between posts. The apparent free energy barriers are comparable to theoretical estimates determined from polymer conformational fluctuations.

Implantable liquid metal-based flexible neural microelectrode array and its application in recovering animal locomotion functions

NASA Astrophysics Data System (ADS)

Guo, Rui; Liu, Jing

2017-10-01

With significant advantages in rapidly restoring the nerve function, electrical stimulation of nervous tissue is a crucial treatment of peripheral nerve injuries leading to common movement disorder. However, the currently available stimulating electrodes generally based on rigid conductive materials would cause a potential mechanical mismatch with soft neural tissues which thus reduces long-term effects of electrical stimulation. Here, we proposed and fabricated a flexible neural microelectrode array system based on the liquid metal GaIn alloy (75.5% Ga and 24.5% In by weight) and via printing approach. Such an alloy with a unique low melting point (10.35 °C) owns excellent electrical conductivity and high compliance, which are beneficial to serve as implantable flexible neural electrodes. The flexible neural microelectrode array embeds four liquid metal electrodes and stretchable interconnects in a PDMS membrane (500 µm in thickness) that possess a lower elastic modulus (1.055 MPa), which is similar to neural tissues with elastic moduli in the 0.1-1.5 MPa range. The electrical experiments indicate that the liquid metal interconnects could sustain over 7000 mechanical stretch cycles with resistance approximately staying at 4 Ω. Over the conceptual experiments on animal sciatic nerve electrical stimulation, the dead bullfrog implanted with flexible neural microelectrode array could even rhythmically contract and move its lower limbs under the electrical stimulations from the implant. This demonstrates a highly efficient way for quickly recovering biological nerve functions. Further, the good biocompatibility of the liquid metal material was justified via a series of biological experiments. This liquid metal modality for neural stimulation is expected to play important roles as biologic electrodes to overcome the fundamental mismatch in mechanics between biological tissues and electronic devices in the coming time.

Effect of LPSO and SFs on microstructure evolution and mechanical properties of Mg-Gd-Y-Zn-Zr alloy

NASA Astrophysics Data System (ADS)

Xu, Chao; Nakata, Taiki; Qiao, Xiaoguang; Zheng, Mingyi; Wu, Kun; Kamado, Shigeharu

2017-01-01

High performance Mg-8.2Gd-3.8Y-1.0Zn-0.4Zr alloy with high strength and excellent ductility has been successfully developed by hot extrusion. The effect of plate-shaped long period stacking ordered (LPSO) phases and solute-segregated stacking faults (SFs) on the dynamically recrystallization (DRX) behavior was analyzed. The plate-shaped LPSO phases stimulate the DRX by particle stimulated nucleation mechanism, leading to higher DRX ratio and weaker basal texture. While for the alloy with dense fine SFs inside the original grains, discontinuous DRX initially occurs at the original grain boundaries, and the DRX is obviously restricted. Consequently, alloy containing dense SFs exhibits higher strength but lower ductility compared with alloy with plated-shaped LPSO phases.

Effect of LPSO and SFs on microstructure evolution and mechanical properties of Mg-Gd-Y-Zn-Zr alloy

PubMed Central

Xu, Chao; Nakata, Taiki; Qiao, Xiaoguang; Zheng, Mingyi; Wu, Kun; Kamado, Shigeharu

2017-01-01

High performance Mg-8.2Gd-3.8Y-1.0Zn-0.4Zr alloy with high strength and excellent ductility has been successfully developed by hot extrusion. The effect of plate-shaped long period stacking ordered (LPSO) phases and solute-segregated stacking faults (SFs) on the dynamically recrystallization (DRX) behavior was analyzed. The plate-shaped LPSO phases stimulate the DRX by particle stimulated nucleation mechanism, leading to higher DRX ratio and weaker basal texture. While for the alloy with dense fine SFs inside the original grains, discontinuous DRX initially occurs at the original grain boundaries, and the DRX is obviously restricted. Consequently, alloy containing dense SFs exhibits higher strength but lower ductility compared with alloy with plated-shaped LPSO phases. PMID:28134297

Conditionally immortalized human pancreatic stellate cell lines demonstrate enhanced proliferation and migration in response to IGF-I

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rosendahl, Ann H., E-mail: ann.rosendahl@med.lu.se; Lund University and Skåne University Hospital, Department of Clinical Sciences Lund, Division of Oncology and Pathology, Lund; Gundewar, Chinmay

2015-01-15

Pancreatic stellate cells (PSCs) play a key role in the dense desmoplastic stroma associated with pancreatic ductal adenocarcinoma. Studies on human PSCs have been minimal due to difficulty in maintaining primary PSC in culture. We have generated the first conditionally immortalized human non-tumor (NPSC) and tumor-derived (TPSC) pancreatic stellate cells via transformation with the temperature-sensitive SV40 large T antigen and human telomerase (hTERT). These cells proliferate at 33°C. After transfer to 37°C, the SV40LT is switched off and the cells regain their primary PSC phenotype and growth characteristics. NPSC contained cytoplasmic vitamin A-storing lipid droplets, while both NPSC and TPSCmore » expressed the characteristic markers αSMA, vimentin, desmin and GFAP. Proteome array analysis revealed that of the 55 evaluated proteins, 27 (49%) were upregulated ≥3-fold in TPSC compared to NPSC, including uPA, pentraxin-3, endoglin and endothelin-1. Two insulin-like growth factor binding proteins (IGFBPs) were inversely expressed. Although discordant IGFBP-2 and IGFBP-3 levels, IGF-I was found to stimulate proliferation of both NPSC and TPSC. Both basal and IGF-I stimulated motility was significantly enhanced in TPSC compared to NPSC. In conclusion, these cells provide a unique resource that will facilitate further study of the active stroma compartment associated with pancreatic cancer. - Highlights: • Generation of human conditionally immortalized human pancreatic stellate cell lines. • Temperature-sensitive SV40LT allows switch to primary PSC phenotype characteristics. • Proteome profiling revealed distinct expression patterns between TPSC and NPSC. • Enhanced IGF-I-stimulated proliferation and motility by TPSC compared to NPSC.« less

Pulse Mode Light Sensing Using Four-Layer Semiconductor Structures and Their Application in Neural Networks

DTIC Science & Technology

2008-12-01

However, the visual sensation was found to occur in retinal areas distant from the implant [10]. Since the current generated under normal light...electronics could limit the use of the microphotodetector array in retinal stimulation. Alternatively, a thin array, containing 64 electrodes...that passes through the skull and skin. Outside the skull, the device is similar to the retinal stimulators, with a television camera mounted on

Optogenetic Stimulation of Peripheral Vagus Nerves using Flexible OLED Display Technology to Treat Chronic Inflammatory Disease and Mental Health Disorders

DTIC Science & Technology

2016-03-31

transcutaneously via the outer ear using a high-resolution, addressable array of organic light emitting diodes (OLEDs) manufactured on a flexible...therapeutic optical stimulation in optogenetically modified neural tissue. Keywords: Optogenetics; neuromodulation; organic light emitting diode ...the outer ear using a high-resolution, two-dimensional (2-D), addressable array of red organic light - emitting diodes (OLEDs) manufactured on a thin

Three dimensional metafilms with dual channel unit cells

DOE PAGES

Burckel, D. Bruce; Campione, Salvatore; Davids, Paul S.; ...

2017-04-04

Three-dimensional (3D) metafilms composed of periodic arrays of silicon unit cells containing single and multiple micrometer-scale vertical split ring resonators (SRRs) per unit cell were fabricated. In contrast to planar and stacked planar structures, these 3D metafilms have a thickness t ~λ d/4, allowing for classical thin film effects in the long wavelength limit. The infrared specular far-field scattering response was measured for metafilms containing one and two resonators per unit cell and compared to numerical simulations. Excellent agreement in the frequency region below the onset of diffractive scattering was obtained. For dense arrays of unit cells containing single SRRs,more » normally incident linearly polarized plane waves which do not excite a resonant response result in thin film interference fringes in the reflected spectra and are virtually indistinguishable from the scattering response of an undecorated array of unit cells. For the resonant linear polarization, the specular reflection for arrays is highly dependent on the SRR orientation on the vertical face for gap-up, gap-down, and gap-right orientations. For dense arrays of unit cells containing two SRRs per unit cell positioned on adjacent faces, the specular reflection spectra are slightly modified due to near-field coupling between the orthogonally oriented SRRs but otherwise exhibit reflection spectra largely representative of the corresponding single-SRR unit cell structures. Lastly, the ability to pack the unit cell with multiple inclusions which can be independently excited by choice of incident polarization suggests the construction of dual-channel films where the scattering response is selected by altering the incident polarization.« less

Ground robotic measurement of aeolian processes

USDA-ARS?s Scientific Manuscript database

Models of aeolian processes rely on accurate measurements of the rates of sediment transport by wind, and careful evaluation of the environmental controls of these processes. Existing field approaches typically require intensive, event-based experiments involving dense arrays of instruments. These d...

Chronic Electrical Stimulation with a Suprachoroidal Retinal Prosthesis: A Preclinical Safety and Efficacy Study

PubMed Central

Nayagam, David A. X.; Williams, Richard A.; Allen, Penelope J.; Shivdasani, Mohit N.; Luu, Chi D.; Salinas-LaRosa, Cesar M.; Finch, Sue; Ayton, Lauren N.; Saunders, Alexia L.; McPhedran, Michelle; McGowan, Ceara; Villalobos, Joel; Fallon, James B.; Wise, Andrew K.; Yeoh, Jonathan; Xu, Jin; Feng, Helen; Millard, Rodney; McWade, Melanie; Thien, Patrick C.; Williams, Chris E.; Shepherd, Robert K.

2014-01-01

Purpose To assess the safety and efficacy of chronic electrical stimulation of the retina with a suprachoroidal visual prosthesis. Methods Seven normally-sighted feline subjects were implanted for 96–143 days with a suprachoroidal electrode array and six were chronically stimulated for 70–105 days at levels that activated the visual cortex. Charge balanced, biphasic, current pulses were delivered to platinum electrodes in a monopolar stimulation mode. Retinal integrity/function and the mechanical stability of the implant were assessed monthly using electroretinography (ERG), optical coherence tomography (OCT) and fundus photography. Electrode impedances were measured weekly and electrically-evoked visual cortex potentials (eEVCPs) were measured monthly to verify that chronic stimuli were suprathreshold. At the end of the chronic stimulation period, thresholds were confirmed with multi-unit recordings from the visual cortex. Randomized, blinded histological assessments were performed by two pathologists to compare the stimulated and non-stimulated retina and adjacent tissue. Results All subjects tolerated the surgical and stimulation procedure with no evidence of discomfort or unexpected adverse outcomes. After an initial post-operative settling period, electrode arrays were mechanically stable. Mean electrode impedances were stable between 11–15 kΩ during the implantation period. Visually-evoked ERGs & OCT were normal, and mean eEVCP thresholds did not substantially differ over time. In 81 of 84 electrode-adjacent tissue samples examined, there were no discernible histopathological differences between stimulated and unstimulated tissue. In the remaining three tissue samples there were minor focal fibroblastic and acute inflammatory responses. Conclusions Chronic suprathreshold electrical stimulation of the retina using a suprachoroidal electrode array evoked a minimal tissue response and no adverse clinical or histological findings. Moreover, thresholds and electrode impedance remained stable for stimulation durations of up to 15 weeks. This study has demonstrated the safety and efficacy of suprachoroidal stimulation with charge balanced stimulus currents. PMID:24853376

The use of hydrogel as an electrode-skin interface for electrode array FES applications.

PubMed

Cooper, Glen; Barker, Anthony T; Heller, Ben W; Good, Tim; Kenney, Laurence P J; Howard, David

2011-10-01

Functional electrical stimulation is commonly used to restore function in post-stroke patients in upper and lower limb applications. Location of the electrodes can be a problem hence some research groups have begun to experiment with electrode arrays. Electrode arrays are interfaced with a thin continuous hydrogel sheet which is high resistivity to reduce transverse currents between electrodes in the array. Research using electrode arrays has all been conducted in a laboratory environment over short time periods but it is suspected that this approach will not be feasible over longer time periods due to changes in hydrogel resistivity. High resistivity hydrogel samples were tested by leaving them in contact with the skin over a seven day period. The samples became extremely conductive with resistivities reaching around 10-50 Ωm. The effect of these resistivity changes was studied using finite element analysis to solve for the stationary current quasi-static electric field gradient in the tissue. Electrical stimulation efficiency and focality were calculated for both a high and low resistivity electrode-skin interface layer at different tissue depths. The results showed that low resistivity hydrogel produced significant decreases in stimulation efficiency and focality compared to high resistivity hydrogel. Copyright © 2011 IPEM. Published by Elsevier Ltd. All rights reserved.

Guided neuronal growth on arrays of biofunctionalized GaAs/InGaAs semiconductor microtubes

NASA Astrophysics Data System (ADS)

Bausch, Cornelius S.; Koitmäe, Aune; Stava, Eric; Price, Amanda; Resto, Pedro J.; Huang, Yu; Sonnenberg, David; Stark, Yuliya; Heyn, Christian; Williams, Justin C.; Dent, Erik W.; Blick, Robert H.

2013-10-01

We demonstrate embedded growth of cortical mouse neurons in dense arrays of semiconductor microtubes. The microtubes, fabricated from a strained GaAs/InGaAs heterostructure, guide axon growth through them and potentially enable electrical and optical probing of propagating action potentials. The coaxial nature of the microtubes—similar to myelin—is expected to enhance the signal transduction along the axon. We present a technique of suppressing arsenic toxicity and prove the success of this technique by overgrowing neuronal mouse cells.

Nanofabrication of Arrays of Silicon Field Emitters with Vertical Silicon Nanowire Current Limiters and Self-Aligned Gates

DTIC Science & Technology

2016-08-19

in a dielectric matrix. This paper explores the electronic device applications of dense arrays of silicon nanowires that are embedded in Nanotechnology ... Nanotechnology 27 (2016) 295302 (11pp) doi:10.1088/0957-4484/27/29/295302 Original content from this work may be used under the terms of the Creative...compared 2 Nanotechnology 27 (2016) 295302 S A Guerrera and A I Akinwande to the device reported by Velasquez-Garcia et al, but it also reduces the

A multichannel scala tympani electrode array incorporating a drug delivery system for chronic intracochlear infusion.

PubMed

Shepherd, Robert K; Xu, Jin

2002-10-01

We have developed a novel scala tympani electrode array suitable for use in experimental animals. A unique feature of this array is its ability to chronically deliver pharmacological agents to the scala tympani. The design of the electrode array is described in detail. Experimental studies performed in guinea pigs confirm that this array can successfully deliver various drugs to the cochlea while chronically stimulating the auditory nerve.

Demultiplexer circuit for neural stimulation

DOEpatents

Wessendorf, Kurt O; Okandan, Murat; Pearson, Sean

2012-10-09

A demultiplexer circuit is disclosed which can be used with a conventional neural stimulator to extend the number of electrodes which can be activated. The demultiplexer circuit, which is formed on a semiconductor substrate containing a power supply that provides all the dc electrical power for operation of the circuit, includes digital latches that receive and store addressing information from the neural stimulator one bit at a time. This addressing information is used to program one or more 1:2.sup.N demultiplexers in the demultiplexer circuit which then route neural stimulation signals from the neural stimulator to an electrode array which is connected to the outputs of the 1:2.sup.N demultiplexer. The demultiplexer circuit allows the number of individual electrodes in the electrode array to be increased by a factor of 2.sup.N with N generally being in a range of 2-4.

Replication-guided nucleosome packing and nucleosome breathing expedite the formation of dense arrays

PubMed Central

Osberg, Brendan; Nuebler, Johannes; Korber, Philipp; Gerland, Ulrich

2014-01-01

The first level of genome packaging in eukaryotic cells involves the formation of dense nucleosome arrays, with DNA coverage near 90% in yeasts. How cells achieve such high coverage within a short time, e.g. after DNA replication, remains poorly understood. It is known that random sequential adsorption of impenetrable particles on a line reaches high density extremely slowly, due to a jamming phenomenon. The nucleosome-shifting action of remodeling enzymes has been proposed as a mechanism to resolve such jams. Here, we suggest two biophysical mechanisms which assist rapid filling of DNA with nucleosomes, and we quantitatively characterize these mechanisms within mathematical models. First, we show that the ‘softness’ of nucleosomes, due to nucleosome breathing and stepwise nucleosome assembly, significantly alters the filling behavior, speeding up the process relative to ‘hard’ particles with fixed, mutually exclusive DNA footprints. Second, we explore model scenarios in which the progression of the replication fork could eliminate nucleosome jamming, either by rapid filling in its wake or via memory of the parental nucleosome positions. Taken together, our results suggest that biophysical effects promote rapid nucleosome filling, making the reassembly of densely packed nucleosomes after DNA replication a simpler task for cells than was previously thought. PMID:25428353

Development of nanowire arrays for neural probe

NASA Astrophysics Data System (ADS)

Abraham, Jose K.; Xie, Jining; Varadan, Vijay K.

2005-05-01

It is already established that functional electrical stimulation is an effective way to restore many functions of the brain in disabled individuals. The electrical stimulation can be done by using an array of electrodes. Neural probes stimulate or sense the biopotentials mainly through the exposed metal sites. These sites should be smaller relative to the spatial potential distribution so that any potential averaging in the sensing area can be avoided. At the same time, the decrease in size of these sensing sites is limited due to the increase in impedance levels and the thermal noise while decreasing its size. It is known that current density in a planar electrode is not uniform and a higher current density can be observer around the perimeter of the electrodes. Electrical measurements conducted on many nanotubes and nanowires have already proved that it could be possible to use for current density applications and the drawbacks of the present design in neural probes can be overcome by incorporating many nanotechnology solutions. In this paper we present the design and development of nanowire arrays for the neural probe for the multisite contact which has the ability to collect and analyze isolated single unit activity. An array of vertically grown nanowires is used as contact site and many of such arrays can be used for stimulating as well as recording sites. The nanolevel interaction and wireless communication solution can extend to applications involving the treatment of many neurological disorders including Parkinson"s disease, Alzheimer"s disease, spinal injuries and the treatment of blindness and paralyzed patients with minimal or no invasive surgical procedures.

NANOCI-Nanotechnology Based Cochlear Implant With Gapless Interface to Auditory Neurons.

PubMed

Senn, Pascal; Roccio, Marta; Hahnewald, Stefan; Frick, Claudia; Kwiatkowska, Monika; Ishikawa, Masaaki; Bako, Peter; Li, Hao; Edin, Fredrik; Liu, Wei; Rask-Andersen, Helge; Pyykkö, Ilmari; Zou, Jing; Mannerström, Marika; Keppner, Herbert; Homsy, Alexandra; Laux, Edith; Llera, Miguel; Lellouche, Jean-Paul; Ostrovsky, Stella; Banin, Ehud; Gedanken, Aharon; Perkas, Nina; Wank, Ute; Wiesmüller, Karl-Heinz; Mistrík, Pavel; Benav, Heval; Garnham, Carolyn; Jolly, Claude; Gander, Filippo; Ulrich, Peter; Müller, Marcus; Löwenheim, Hubert

2017-09-01

: Cochlear implants (CI) restore functional hearing in the majority of deaf patients. Despite the tremendous success of these devices, some limitations remain. The bottleneck for optimal electrical stimulation with CI is caused by the anatomical gap between the electrode array and the auditory neurons in the inner ear. As a consequence, current devices are limited through 1) low frequency resolution, hence sub-optimal sound quality and 2), large stimulation currents, hence high energy consumption (responsible for significant battery costs and for impeding the development of fully implantable systems). A recently completed, multinational and interdisciplinary project called NANOCI aimed at overcoming current limitations by creating a gapless interface between auditory nerve fibers and the cochlear implant electrode array. This ambitious goal was achieved in vivo by neurotrophin-induced attraction of neurites through an intracochlear gel-nanomatrix onto a modified nanoCI electrode array located in the scala tympani of deafened guinea pigs. Functionally, the gapless interface led to lower stimulation thresholds and a larger dynamic range in vivo, and to reduced stimulation energy requirement (up to fivefold) in an in vitro model using auditory neurons cultured on multi-electrode arrays. In conclusion, the NANOCI project yielded proof of concept that a gapless interface between auditory neurons and cochlear implant electrode arrays is feasible. These findings may be of relevance for the development of future CI systems with better sound quality and performance and lower energy consumption. The present overview/review paper summarizes the NANOCI project history and highlights achievements of the individual work packages.

Simultaneous recording of mouse retinal ganglion cells during epiretinal or subretinal stimulation

PubMed Central

Sim, S.L.; Szalewski, R.J.; Johnson, L.J.; Akah, L.E.; Shoemaker, L.E.; Thoreson, W.B.; Margalit, E.

2015-01-01

We compared response patterns and electrical receptive fields (ERF) of retinal ganglion cells (RGCs) during epiretinal and subretinal electrical stimulation of isolated mouse retina. Retinas were stimulated with an array of 3200 independently controllable electrodes. Four response patterns were observed: a burst of activity immediately after stimulation (Type I cells, Vision Research (2008), 48, 1562–1568), delayed bursts beginning >25 ms after stimulation (Type II), a combination of both (Type III), and inhibition of ongoing spike activity. Type I responses were produced more often by epiretinal than subretinal stimulation whereas delayed and inhibitory responses were evoked more frequently by subretinal stimulation. Response latencies were significantly shorter with epiretinal than subretinal stimulation. These data suggest that subretinal stimulation is more effective at activating intraretinal circuits than epiretinal stimulation. There was no significant difference in charge threshold between subretinal and epiretinal configurations. ERFs were defined by the stimulating array surface area that successfully stimulated spikes in an RGC. ERFs were complex in shape, similar to receptive fields mapped with light. ERF areas were significantly smaller with subretinal than epiretinal stimulation. This may reflect the greater distance between stimulating electrodes and RGCs in the subretinal configuration. ERFs for immediate and delayed responses mapped within the same Type III cells differed in shape and size, consistent with different sites and mechanisms for generating these two response types. PMID:24863584

Computationally optimized ECoG stimulation with local safety constraints.

PubMed

Guler, Seyhmus; Dannhauer, Moritz; Roig-Solvas, Biel; Gkogkidis, Alexis; Macleod, Rob; Ball, Tonio; Ojemann, Jeffrey G; Brooks, Dana H

2018-06-01

Direct stimulation of the cortical surface is used clinically for cortical mapping and modulation of local activity. Future applications of cortical modulation and brain-computer interfaces may also use cortical stimulation methods. One common method to deliver current is through electrocorticography (ECoG) stimulation in which a dense array of electrodes are placed subdurally or epidurally to stimulate the cortex. However, proximity to cortical tissue limits the amount of current that can be delivered safely. It may be desirable to deliver higher current to a specific local region of interest (ROI) while limiting current to other local areas more stringently than is guaranteed by global safety limits. Two commonly used global safety constraints bound the total injected current and individual electrode currents. However, these two sets of constraints may not be sufficient to prevent high current density locally (hot-spots). In this work, we propose an efficient approach that prevents current density hot-spots in the entire brain while optimizing ECoG stimulus patterns for targeted stimulation. Specifically, we maximize the current along a particular desired directional field in the ROI while respecting three safety constraints: one on the total injected current, one on individual electrode currents, and the third on the local current density magnitude in the brain. This third set of constraints creates a computational barrier due to the huge number of constraints needed to bound the current density at every point in the entire brain. We overcome this barrier by adopting an efficient two-step approach. In the first step, the proposed method identifies the safe brain region, which cannot contain any hot-spots solely based on the global bounds on total injected current and individual electrode currents. In the second step, the proposed algorithm iteratively adjusts the stimulus pattern to arrive at a solution that exhibits no hot-spots in the remaining brain. We report on simulations on a realistic finite element (FE) head model with five anatomical ROIs and two desired directional fields. We also report on the effect of ROI depth and desired directional field on the focality of the stimulation. Finally, we provide an analysis of optimization runtime as a function of different safety and modeling parameters. Our results suggest that optimized stimulus patterns tend to differ from those used in clinical practice. Copyright © 2018 Elsevier Inc. All rights reserved.

Ambient seismic noise monitoring of the Super-Sauze landslide from a very dense temporary seismic array

NASA Astrophysics Data System (ADS)

Chtouki, Toufik; Vergne, Jerome; Provost, Floriane; Malet, Jean-Philippe; Burtin, Arnaud; Hibert, Clément

2017-04-01

The Super-Sauze landslide is located on the southern part of the Barcelonnette Basin (French Alps) and has developed in a soft clay-shale environment. It is one of the four sites continuously monitored through a wide variety of geophysical and hydro-geological techniques in the framework of the OMIV French national landslide observatory. From early June to mid-July 2016, a temporary dense seismic array has been installed in the most active part of the landslide and at its surroundings. 50 different sites with an average inter-station distance of 50m have been instrumented with 150 miniaturized and autonomous seismic stations (Zland nodes), allowing a continuous record of the seismic signal at frequencies higher than 0.2Hz over an almost regular grid. Concurrently, a Ground-Based InSAR device allowed for a precise and continuous monitoring of the surface deformation. Overall, this experiment is intended to better characterize the spatio-temporal evolution of the deformation processes related to various type of forcing. We analyze the continuous records of ambient seismic noise recorded by the dense array. Using power spectral densities, we characterize the various types of natural and anthropogenic seismic sources, including the effect of water turbulence and bedload transport in the small nearby torrents. We also compute the correlation of the ambient diffuse seismic noise in various frequency bands for the 2448 station pairs to recover the empirical Green functions between them. The temporal evolution of the coda part of these noise correlation functions allows monitoring and localizing shear wave velocity variations in the sliding mass. Here we present some preliminary results of this analysis and compare the seismic variations to meteorological data and surface deformation.

Carbon nanotubes on a substrate

DOEpatents

Gao, Yufei [Kennewick, WA; Liu, Jun [West Richland, WA

2002-03-26

The present invention includes carbon nanotubes whose hollow cores are 100% filled with conductive filler. The carbon nanotubes are in uniform arrays on a conductive substrate and are well-aligned and can be densely packed. The uniformity of the carbon nanotube arrays is indicated by the uniform length and diameter of the carbon nanotubes, both which vary from nanotube to nanotube on a given array by no more than about 5%. The alignment of the carbon nanotubes is indicated by the perpendicular growth of the nanotubes from the substrates which is achieved in part by the simultaneous growth of the conductive filler within the hollow core of the nanotube and the densely packed growth of the nanotubes. The present invention provides a densely packed carbon nanotube growth where each nanotube is in contact with at least one nearest-neighbor nanotube. The substrate is a conductive substrate coated with a growth catalyst, and the conductive filler can be single crystals of carbide formed by a solid state reaction between the substrate material and the growth catalyst. The present invention further provides a method for making the filled carbon nanotubes on the conductive substrates. The method includes the steps of depositing a growth catalyst onto the conductive substrate as a prepared substrate, creating a vacuum within a vessel which contains the prepared substrate, flowing H2/inert (e.g. Ar) gas within the vessel to increase and maintain the pressure within the vessel, increasing the temperature of the prepared substrate, and changing the H2/Ar gas to ethylene gas such that the ethylene gas flows within the vessel. Additionally, varying the density and separation of the catalyst particles on the conductive substrate can be used to control the diameter of the nanotubes.

Method of making carbon nanotubes on a substrate

DOEpatents

Gao, Yufei; Liu, Jun

2006-03-14

The present invention includes carbon nanotubes whose hollow cores are 100% filled with conductive filler. The carbon nanotubes are in uniform arrays on a conductive substrate and are well-aligned and can be densely packed. The uniformity of the carbon nanotube arrays is indicated by the uniform length and diameter of the carbon nanotubes, both which vary from nanotube to nanotube on a given array by no more than about 5%. The alignment of the carbon nanotubes is indicated by the perpendicular growth of the nanotubes from the substrates which is achieved in part by the simultaneous growth of the conductive filler within the hollow core of the nanotube and the densely packed growth of the nanotubes. The present invention provides a densely packed carbon nanotube growth where each nanotube is in contact with at least one nearest-neighbor nanotube. The substrate is a conductive substrate coated with a growth catalyst, and the conductive filler can be single crystals of carbide formed by a solid state reaction between the substrate material and the growth catalyst. The present invention further provides a method for making the filled carbon nanotubes on the conductive substrates. The method includes the steps of depositing a growth catalyst onto the conductive substrate as a prepared substrate, creating a vacuum within a vessel which contains the prepared substrate, flowing H2/inert (e.g. Ar) gas within the vessel to increase and maintain the pressure within the vessel, increasing the temperature of the prepared substrate, and changing the H2/Ar gas to ethylene gas such that the ethylene gas flows within the vessel. Additionally, varying the density and separation of the catalyst particles on the conductive substrate can be used to control the diameter of the nanotubes.

Systematic detection of seismic events at Mount St. Helens with an ultra-dense array

NASA Astrophysics Data System (ADS)

Meng, X.; Hartog, J. R.; Schmandt, B.; Hotovec-Ellis, A. J.; Hansen, S. M.; Vidale, J. E.; Vanderplas, J.

2016-12-01

During the summer of 2014, an ultra-dense array of 900 geophones was deployed around the crater of Mount St. Helens and continuously operated for 15 days. This dataset provides us an unprecedented opportunity to systematically detect seismic events around an active volcano and study their underlying mechanisms. We use a waveform-based matched filter technique to detect seismic events from this dataset. Due to the large volume of continuous data ( 1 TB), we performed the detection on the GPU cluster Stampede (https://www.tacc.utexas.edu/systems/stampede). We build a suite of template events from three catalogs: 1) the standard Pacific Northwest Seismic Network (PNSN) catalog (45 events); 2) the catalog from Hansen&Schmandt (2015) obtained with a reverse-time imaging method (212 events); and 3) the catalog identified with a matched filter technique using the PNSN permanent stations (190 events). By searching for template matches in the ultra-dense array, we find 2237 events. We then calibrate precise relative magnitudes for template and detected events, using a principal component fit to measure waveform amplitude ratios. The magnitude of completeness and b-value of the detected catalog is -0.5 and 1.1, respectively. Our detected catalog shows several intensive swarms, which are likely driven by fluid pressure transients in conduits or slip transients on faults underneath the volcano. We are currently relocating the detected catalog with HypoDD and measuring the seismic velocity changes at Mount St. Helens using the coda wave interferometry of detected repeating earthquakes. The accurate temporal-spatial migration pattern of seismicity and seismic property changes should shed light on the physical processes beneath Mount St. Helens.

High-density CMOS Microelectrode Array System for Impedance Spectroscopy and Imaging of Biological Cells.

PubMed

Vijay, Viswam; Raziyeh, Bounik; Amir, Shadmani; Jelena, Dragas; Alicia, Boos Julia; Axel, Birchler; Jan, Müller; Yihui, Chen; Andreas, Hierlemann

2017-01-26

A monolithic measurement platform was implemented to enable label-free in-vitro electrical impedance spectroscopy measurements of cells on multi-functional CMOS microelectrode array. The array includes 59,760 platinum microelectrodes, densely packed within a 4.5 mm × 2.5 mm sensing region at a pitch of 13.5 μm. The 32 on-chip lock-in amplifiers can be used to measure the impedance of any arbitrarily chosen electrodes on the array by applying a sinusoidal voltage, generated by an on-chip waveform generator with a frequency range from 1 Hz to 1 MHz, and measuring the respective current. Proof-of-concept measurements of impedance sensing and imaging are shown in this paper. Correlations between cell detection through optical microscopy and electrochemical impedance scanning were established.

The Extensive Air Shower Experiment Kascade-Grande

NASA Astrophysics Data System (ADS)

Kang, Donghwa; Apel, W. D.; Arteaga, J. C.; Badea, F.; Bekk, K.; Bertaina, M.; Blümer, J.; Bozdog, H.; Brancus, I. M.; Brüggemann, M.; Buchholz, P.; Cantoni, E.; Chiavassa, A.; Cossavella, F.; Daumiller, K.; de Souza, V.; di Pierro, F.; Doll, P.; Engel, R.; Engler, J.; Finger, M.; Fuhrmann, D.; Ghia, P. L.; Gils, H. J.; Glasstetter, R.; Grupen, C.; Haungs, A.; Heck, D.; Hörandel, J. R.; Huege, T.; Isar, P. G.; Kampert, K.-H.; Kickelbick, D.; Klages, H. O.; Kolotaev, Y.; Łuczak, P.; Mathes, H. J.; Mayer, H. J.; Milke, J.; Mitrica, B.; Morello, C.; Navarra, G.; Nehls, S.; Oehlschläger, J.; Ostapchenko, S.; Over, S.; Petcu, M.; Pierog, T.; Rebel, H.; Roth, M.; Schatz, G.; Schieler, H.; Schröder, F.; Sima, O.; Stümpert, M.; Toma, G.; Trinchero, G. C.; Ulrich, H.; van Buren, J.; Walkowiak, W.; Weindl, A.; Wochele, J.; Wommer, M.; Zabierowski, J.

The extensive air shower experiment KASCADE-Grande (KArlsruhe Shower Core and Array DEtector and Grande array) is located on site of the Forschungszentrum Karlsruhe in Germany. The original KASCADE experiment consisted of a densely packed scintillator array with unshielded and shielded detectors for the measurement of the electromagnetic and muonic shower component independently, as well as muon tracking devices and a hadron calorimeter. The Grande array as an extension of KASCADE consists of 37 scintillation detector stations covering an area of 700×700 m2. The main goal for the combined measurements of KASCADE and Grande is the investigation of the energy spectrum and composition of primary cosmic rays in the energy range of 1016 to 1018 eV. In this paper an overview of the KASCADE-Grande experiment and recent results will be presented.

Evaluation of focused multipolar stimulation for cochlear implants: a preclinical safety study

NASA Astrophysics Data System (ADS)

Shepherd, Robert K.; Wise, Andrew K.; Enke, Ya Lang; Carter, Paul M.; Fallon, James B.

2017-08-01

Objective. Cochlear implants (CIs) have a limited number of independent stimulation channels due to the highly conductive nature of the fluid-filled cochlea. Attempts to develop highly focused stimulation to improve speech perception in CI users includes the use of simultaneous stimulation via multiple current sources. Focused multipolar (FMP) stimulation is an example of this approach and has been shown to reduce interaction between stimulating channels. However, compared with conventional biphasic current pulses generated from a single current source, FMP is a complex stimulus that includes extended periods of stimulation before charge recovery is achieved, raising questions on whether chronic stimulation with this strategy is safe. The present study evaluated the long-term safety of intracochlear stimulation using FMP in a preclinical animal model of profound deafness. Approach. Six cats were bilaterally implanted with scala tympani electrode arrays two months after deafening, and received continuous unilateral FMP stimulation at levels that evoked a behavioural response for periods of up to 182 d. Electrode impedance, electrically-evoked compound action potentials (ECAPs) and auditory brainstem responses (EABRs) were monitored periodically over the course of the stimulation program from both the stimulated and contralateral control cochleae. On completion of the stimulation program cochleae were examined histologically and the electrode arrays were evaluated for evidence of platinum (Pt) corrosion. Main results. There was no significant difference in electrode impedance between control and chronically stimulated electrodes following long-term FMP stimulation. Moreover, there was no significant difference between ECAP and EABR thresholds evoked from control or stimulated cochleae at either the onset of stimulation or at completion of the stimulation program. Chronic FMP stimulation had no effect on spiral ganglion neuron (SGN) survival when compared with unstimulated control cochleae. Long-term implantation typically evoked a mild foreign body reaction proximal to the electrode array; however stimulated cochleae exhibited a small but statistically significant increase in the tissue response. Finally, there was no evidence of Pt corrosion following long-term FMP stimulation; stimulated electrodes exhibited the same surface features as the unstimulated control electrodes. Significance. Chronic intracochlear FMP stimulation at levels used in the present study did not adversely affect electrically-evoked neural thresholds or SGN survival but evoked a small, benign increase in inflammatory response compared to control ears. Moreover chronic FMP stimulation does not affect the surface of Pt electrodes at suprathreshold stimulus levels. These findings support the safe clinical application of an FMP stimulation strategy.

TLR4- and TRIF-dependent stimulation of B lymphocytes by peptide liposomes enables T cell-independent isotype switch in mice.

PubMed

Pihlgren, Maria; Silva, Alberto B; Madani, Rime; Giriens, Valérie; Waeckerle-Men, Ying; Fettelschoss, Antonia; Hickman, David T; López-Deber, María Pilar; Ndao, Dorin Mlaki; Vukicevic, Marija; Buccarello, Anna Lucia; Gafner, Valérie; Chuard, Nathalie; Reis, Pedro; Piorkowska, Kasia; Pfeifer, Andrea; Kündig, Thomas M; Muhs, Andreas; Johansen, Pål

2013-01-03

Immunoglobulin class switching from IgM to IgG in response to peptides is generally T cell-dependent and vaccination in T cell-deficient individuals is inefficient. We show that a vaccine consisting of a dense array of peptides on liposomes induced peptide-specific IgG responses totally independent of T-cell help. Independency was confirmed in mice lacking T cells and in mice deficient for MHC class II, CD40L, and CD28. The IgG titers were high, long-lived, and comparable with titers obtained in wild-type animals, and the antibody response was associated with germinal center formation, expression of activation-induced cytidine deaminase, and affinity maturation. The T cell-independent (TI) IgG response was strictly dependent on ligation of TLR4 receptors on B cells, and concomitant TLR4 and cognate B-cell receptor stimulation was required on a single-cell level. Surprisingly, the IgG class switch was mediated by TIR-domain-containing adapter inducing interferon-β (TRIF), but not by MyD88. This study demonstrates that peptides can induce TI isotype switching when antigen and TLR ligand are assembled and appropriately presented directly to B lymphocytes. A TI vaccine could enable efficient prophylactic and therapeutic vaccination of patients with T-cell deficiencies and find application in diseases where induction of T-cell responses contraindicates vaccination, for example, in Alzheimer disease.

Restoring motor control and sensory feedback in people with upper extremity amputations using arrays of 96 microelectrodes implanted in the median and ulnar nerves.

PubMed

Davis, T S; Wark, H A C; Hutchinson, D T; Warren, D J; O'Neill, K; Scheinblum, T; Clark, G A; Normann, R A; Greger, B

2016-06-01

An important goal of neuroprosthetic research is to establish bidirectional communication between the user and new prosthetic limbs that are capable of controlling >20 different movements. One strategy for achieving this goal is to interface the prosthetic limb directly with efferent and afferent fibres in the peripheral nervous system using an array of intrafascicular microelectrodes. This approach would provide access to a large number of independent neural pathways for controlling high degree-of-freedom prosthetic limbs, as well as evoking multiple-complex sensory percepts. Utah Slanted Electrode Arrays (USEAs, 96 recording/stimulating electrodes) were implanted for 30 days into the median (Subject 1-M, 31 years post-amputation) or ulnar (Subject 2-U, 1.5 years post-amputation) nerves of two amputees. Neural activity was recorded during intended movements of the subject's phantom fingers and a linear Kalman filter was used to decode the neural data. Microelectrode stimulation of varying amplitudes and frequencies was delivered via single or multiple electrodes to investigate the number, size and quality of sensory percepts that could be evoked. Device performance over time was assessed by measuring: electrode impedances, signal-to-noise ratios (SNRs), stimulation thresholds, number and stability of evoked percepts. The subjects were able to proportionally, control individual fingers of a virtual robotic hand, with 13 different movements decoded offline (r = 0.48) and two movements decoded online. Electrical stimulation across one USEA evoked >80 sensory percepts. Varying the stimulation parameters modulated percept quality. Devices remained intrafascicularly implanted for the duration of the study with no significant changes in the SNRs or percept thresholds. This study demonstrated that an array of 96 microelectrodes can be implanted into the human peripheral nervous system for up to 1 month durations. Such an array could provide intuitive control of a virtual prosthetic hand with broad sensory feedback.

Restoring motor control and sensory feedback in people with upper extremity amputations using arrays of 96 microelectrodes implanted in the median and ulnar nerves

NASA Astrophysics Data System (ADS)

Davis, T. S.; Wark, H. A. C.; Hutchinson, D. T.; Warren, D. J.; O'Neill, K.; Scheinblum, T.; Clark, G. A.; Normann, R. A.; Greger, B.

2016-06-01

Objective. An important goal of neuroprosthetic research is to establish bidirectional communication between the user and new prosthetic limbs that are capable of controlling >20 different movements. One strategy for achieving this goal is to interface the prosthetic limb directly with efferent and afferent fibres in the peripheral nervous system using an array of intrafascicular microelectrodes. This approach would provide access to a large number of independent neural pathways for controlling high degree-of-freedom prosthetic limbs, as well as evoking multiple-complex sensory percepts. Approach. Utah Slanted Electrode Arrays (USEAs, 96 recording/stimulating electrodes) were implanted for 30 days into the median (Subject 1-M, 31 years post-amputation) or ulnar (Subject 2-U, 1.5 years post-amputation) nerves of two amputees. Neural activity was recorded during intended movements of the subject’s phantom fingers and a linear Kalman filter was used to decode the neural data. Microelectrode stimulation of varying amplitudes and frequencies was delivered via single or multiple electrodes to investigate the number, size and quality of sensory percepts that could be evoked. Device performance over time was assessed by measuring: electrode impedances, signal-to-noise ratios (SNRs), stimulation thresholds, number and stability of evoked percepts. Main results. The subjects were able to proportionally, control individual fingers of a virtual robotic hand, with 13 different movements decoded offline (r = 0.48) and two movements decoded online. Electrical stimulation across one USEA evoked >80 sensory percepts. Varying the stimulation parameters modulated percept quality. Devices remained intrafascicularly implanted for the duration of the study with no significant changes in the SNRs or percept thresholds. Significance. This study demonstrated that an array of 96 microelectrodes can be implanted into the human peripheral nervous system for up to 1 month durations. Such an array could provide intuitive control of a virtual prosthetic hand with broad sensory feedback.

Rapid Synthesis of Thin and Long Mo17O47 Nanowire-Arrays in an Oxygen Deficient Flame

PubMed Central

Allen, Patrick; Cai, Lili; Zhou, Lite; Zhao, Chenqi; Rao, Pratap M.

2016-01-01

Mo17O47 nanowire-arrays are promising active materials and electrically-conductive supports for batteries and other devices. While high surface area resulting from long, thin, densely packed nanowires generally leads to improved performance in a wide variety of applications, the Mo17O47 nanowire-arrays synthesized previously by electrically-heated chemical vapor deposition under vacuum conditions were relatively thick and short. Here, we demonstrate a method to grow significantly thinner and longer, densely packed, high-purity Mo17O47 nanowire-arrays with diameters of 20–60 nm and lengths of 4–6 μm on metal foil substrates using rapid atmospheric flame vapor deposition without any chamber or walls. The atmospheric pressure and 1000 °C evaporation temperature resulted in smaller diameters, longer lengths and order-of-magnitude faster growth rate than previously demonstrated. As explained by kinetic and thermodynamic calculations, the selective synthesis of high-purity Mo17O47 nanowires is achieved due to low oxygen partial pressure in the flame products as a result of the high ratio of fuel to oxidizer supplied to the flame, which enables the correct ratio of MoO2 and MoO3 vapor concentrations for the growth of Mo17O47. This flame synthesis method is therefore a promising route for the growth of composition-controlled one-dimensional metal oxide nanomaterials for many applications. PMID:27271194

Ultra-high-aspect-orthogonal and tunable three dimensional polymeric nanochannel stack array for BioMEMS applications

NASA Astrophysics Data System (ADS)

Heo, Joonseong; Kwon, Hyukjin J.; Jeon, Hyungkook; Kim, Bumjoo; Kim, Sung Jae; Lim, Geunbae

2014-07-01

Nanofabrication technologies have been a strong advocator for new scientific fundamentals that have never been described by traditional theory, and have played a seed role in ground-breaking nano-engineering applications. In this study, we fabricated ultra-high-aspect (~106 with O(100) nm nanochannel opening and O(100) mm length) orthogonal nanochannel array using only polymeric materials. Vertically aligned nanochannel arrays in parallel can be stacked to form a dense nano-structure. Due to the flexibility and stretchability of the material, one can tune the size and shape of the nanochannel using elongation and even roll the stack array to form a radial-uniformly distributed nanochannel array. The roll can be cut at discretionary lengths for incorporation with a micro/nanofluidic device. As examples, we demonstrated ion concentration polarization with the device for Ohmic-limiting/overlimiting current-voltage characteristics and preconcentrated charged species. The density of the nanochannel array was lower than conventional nanoporous membranes, such as anodic aluminum oxide membranes (AAO). However, accurate controllability over the nanochannel array dimensions enabled multiplexed one microstructure-on-one nanostructure interfacing for valuable biological/biomedical microelectromechanical system (BioMEMS) platforms, such as nano-electroporation.Nanofabrication technologies have been a strong advocator for new scientific fundamentals that have never been described by traditional theory, and have played a seed role in ground-breaking nano-engineering applications. In this study, we fabricated ultra-high-aspect (~106 with O(100) nm nanochannel opening and O(100) mm length) orthogonal nanochannel array using only polymeric materials. Vertically aligned nanochannel arrays in parallel can be stacked to form a dense nano-structure. Due to the flexibility and stretchability of the material, one can tune the size and shape of the nanochannel using elongation and even roll the stack array to form a radial-uniformly distributed nanochannel array. The roll can be cut at discretionary lengths for incorporation with a micro/nanofluidic device. As examples, we demonstrated ion concentration polarization with the device for Ohmic-limiting/overlimiting current-voltage characteristics and preconcentrated charged species. The density of the nanochannel array was lower than conventional nanoporous membranes, such as anodic aluminum oxide membranes (AAO). However, accurate controllability over the nanochannel array dimensions enabled multiplexed one microstructure-on-one nanostructure interfacing for valuable biological/biomedical microelectromechanical system (BioMEMS) platforms, such as nano-electroporation. Electronic supplementary information (ESI) available. See DOI: 10.1039/c4nr00350k

Extracorporeal Stimulation of Sacral Nerve Roots for Observation of Pelvic Autonomic Nerve Integrity: Description of a Novel Methodological Setup.

PubMed

Moszkowski, Tomasz; Kauff, Daniel W; Wegner, Celine; Ruff, Roman; Somerlik-Fuchs, Karin H; Kruger, Thilo B; Augustyniak, Piotr; Hoffmann, Klaus-Peter; Kneist, Werner

2018-03-01

Neurophysiologic monitoring can improve autonomic nerve sparing during critical phases of rectal cancer surgery. To develop a system for extracorporeal stimulation of sacral nerve roots. Dedicated software controlled a ten-electrode stimulation array by switching between different electrode configurations and current levels. A built-in impedance and current level measurement assessed the effectiveness of current injection. Intra-anal surface electromyography (sEMG) informed on targeting the sacral nerve roots. All tests were performed on five pig specimens. During switching between electrode configurations, the system delivered 100% of the set current (25 mA, 30 Hz, 200 μs cathodic pulses) in 93% of 250 stimulation trains across all specimens. The impedance measured between single stimulation array contacts and corresponding anodes across all electrode configurations and specimens equaled 3.7 ± 2.5 kΩ. The intra-anal sEMG recorded a signal amplitude increase as previously observed in the literature. When the stimulation amplitude was tested in the range from 1 to 21 mA using the interconnected contacts of the stimulation array and the intra-anal anode, the impedance remained below 250 Ω and the system delivered 100% of the set current in all cases. Intra-anal sEMG showed an amplitude increase for current levels exceeding 6 mA. The system delivered stable electric current, which was proved by built-in impedance and current level measurements. Intra-anal sEMG confirmed the ability to target the branches of the autonomous nervous system originating from the sacral nerve roots. Stimulation outside of the operative field during rectal cancer surgery is feasible and may improve the practicality of pelvic intraoperative neuromonitoring.

Directed self-assembly of block copolymers for nanolithography: fabrication of isolated features and essential integrated circuit geometries.

PubMed

Stoykovich, Mark P; Kang, Huiman; Daoulas, Kostas Ch; Liu, Guoliang; Liu, Chi-Chun; de Pablo, Juan J; Müller, Marcus; Nealey, Paul F

2007-10-01

Self-assembling block copolymers are of interest for nanomanufacturing due to the ability to realize sub-100 nm dimensions, thermodynamic control over the size and uniformity and density of features, and inexpensive processing. The insertion point of these materials in the production of integrated circuits, however, is often conceptualized in the short term for niche applications using the dense periodic arrays of spots or lines that characterize bulk block copolymer morphologies, or in the long term for device layouts completely redesigned into periodic arrays. Here we show that the domain structure of block copolymers in thin films can be directed to assemble into nearly the complete set of essential dense and isolated patterns as currently defined by the semiconductor industry. These results suggest that block copolymer materials, with their intrinsically advantageous self-assembling properties, may be amenable for broad application in advanced lithography, including device layouts used in existing nanomanufacturing processes.

Thermoelectrics. Dense dislocation arrays embedded in grain boundaries for high-performance bulk thermoelectrics.

PubMed

Kim, Sang Il; Lee, Kyu Hyoung; Mun, Hyeon A; Kim, Hyun Sik; Hwang, Sung Woo; Roh, Jong Wook; Yang, Dae Jin; Shin, Weon Ho; Li, Xiang Shu; Lee, Young Hee; Snyder, G Jeffrey; Kim, Sung Wng

2015-04-03

The widespread use of thermoelectric technology is constrained by a relatively low conversion efficiency of the bulk alloys, which is evaluated in terms of a dimensionless figure of merit (zT). The zT of bulk alloys can be improved by reducing lattice thermal conductivity through grain boundary and point-defect scattering, which target low- and high-frequency phonons. Dense dislocation arrays formed at low-energy grain boundaries by liquid-phase compaction in Bi(0.5)Sb(1.5)Te3 (bismuth antimony telluride) effectively scatter midfrequency phonons, leading to a substantially lower lattice thermal conductivity. Full-spectrum phonon scattering with minimal charge-carrier scattering dramatically improved the zT to 1.86 ± 0.15 at 320 kelvin (K). Further, a thermoelectric cooler confirmed the performance with a maximum temperature difference of 81 K, which is much higher than current commercial Peltier cooling devices. Copyright © 2015, American Association for the Advancement of Science.

Optimized design and research of secondary microprism for dense array concentrating photovoltaic module

NASA Astrophysics Data System (ADS)

Yang, Guanghui; Chen, Bingzhen; Liu, Youqiang; Guo, Limin; Yao, Shun; Wang, Zhiyong

2015-10-01

As the critical component of concentrating photovoltaic module, secondary concentrators can be effective in increasing the acceptance angle and incident light, as well as improving the energy uniformity of focal spots. This paper presents a design of transmission-type secondary microprism for dense array concentrating photovoltaic module. The 3-D model of this design is established by Solidworks and important parameters such as inclination angle and component height are optimized using Zemax. According to the design and simulation results, several secondary microprisms with different parameters are fabricated and tested in combination with Fresnel lens and multi-junction solar cell. The sun-simulator IV test results show that the combination has the highest output power when secondary microprism height is 5mm and top facet side length is 7mm. Compared with the case without secondary microprism, the output power can improve 11% after the employment of secondary microprisms, indicating the indispensability of secondary microprisms in concentrating photovoltaic module.

Automatic Parallelization of Numerical Python Applications using the Global Arrays Toolkit

DOE Office of Scientific and Technical Information (OSTI.GOV)

Daily, Jeffrey A.; Lewis, Robert R.

2011-11-30

Global Arrays is a software system from Pacific Northwest National Laboratory that enables an efficient, portable, and parallel shared-memory programming interface to manipulate distributed dense arrays. The NumPy module is the de facto standard for numerical calculation in the Python programming language, a language whose use is growing rapidly in the scientific and engineering communities. NumPy provides a powerful N-dimensional array class as well as other scientific computing capabilities. However, like the majority of the core Python modules, NumPy is inherently serial. Using a combination of Global Arrays and NumPy, we have reimplemented NumPy as a distributed drop-in replacement calledmore » Global Arrays in NumPy (GAiN). Serial NumPy applications can become parallel, scalable GAiN applications with only minor source code changes. Scalability studies of several different GAiN applications will be presented showing the utility of developing serial NumPy codes which can later run on more capable clusters or supercomputers.« less

Dense arrays of millimeter-sized glass lenses fabricated at wafer-level.

PubMed

Albero, Jorge; Perrin, Stéphane; Bargiel, Sylwester; Passilly, Nicolas; Baranski, Maciej; Gauthier-Manuel, Ludovic; Bernard, Florent; Lullin, Justine; Froehly, Luc; Krauter, Johann; Osten, Wolfgang; Gorecki, Christophe

2015-05-04

This paper presents the study of a fabrication technique of lenses arrays based on the reflow of glass inside cylindrical silicon cavities. Lenses whose sizes are out of the microfabrication standards are considered. In particular, the case of high fill factor arrays is discussed in detail since the proximity between lenses generates undesired effects. These effects, not experienced when lenses are sufficiently separated so that they can be considered as single items, are corrected by properly designing the silicon cavities. Complete topographic as well as optical characterizations are reported. The compatibility of materials with Micro-Opto-Electromechanical Systems (MOEMS) integration processes makes this technology attractive for the miniaturization of inspection systems, especially those devoted to imaging.

Black GE based on crystalline/amorphous core/shell nanoneedle arrays

DOEpatents

Javey, Ali; Chueh, Yu-Lun; Fan, Zhiyong

2014-03-04

Direct growth of black Ge on low-temperature substrates, including plastics and rubber is reported. The material is based on highly dense, crystalline/amorphous core/shell Ge nanoneedle arrays with ultrasharp tips (.about.4 nm) enabled by the Ni catalyzed vapor-solid-solid growth process. Ge nanoneedle arrays exhibit remarkable optical properties. Specifically, minimal optical reflectance (<1%) is observed, even for high angles of incidence (.about.75.degree.) and for relatively short nanoneedle lengths (.about.1 .mu.m). Furthermore, the material exhibits high optical absorption efficiency with an effective band gap of .about.1 eV. The reported black Ge can have important practical implications for efficient photovoltaic and photodetector applications on nonconventional substrates.

Observations of basin ground motions from a dense seismic array in San Jose, California

USGS Publications Warehouse

Frankel, A.; Carver, D.; Cranswick, E.; Bice, T.; Sell, R.; Hanson, S.

2001-01-01

We installed a dense array of 41 digital seismographs in San Jose, California, to evaluate in detail the effects of a deep sedimentary basin and shallow sedimentary deposits on earthquake ground motions. This urban array is located near the eastern edge of the Santa Clara Valley and spans the Evergreen sedimentary basin identified by gravity data. Average station spacing is 1 km, with three stations initially spaced 110 m apart. Despite the high-noise urban environment, the stations of the array successfully triggered on and recorded small local earthquakes (M 2.5-2.8 at 10-25 km distance) and larger regional events such as the M 5.0 Bolinas earthquake (90 km distance), M 4.6-5.6 earthquakes near Mammoth Lakes (270 km distance), M 4.9-5.6 events in western Nevada (420 km distance) and the M 7.1 Hector Mine earthquake (590 km distance). Maps of spectral ratios across the array show that the highest amplitudes in all frequency bands studied (0.125-8 Hz) are generally observed at stations farther from the eastern edge of the Santa Clara Valley. Larger spectral amplitudes are often observed above the western edge of the Evergreen Basin. Snapshots of the recorded wavefield crossing the array for regional events to the east reveal that large, low-frequency (0.125-0.5 Hz) arrivals after the S-wave travel from south to north across the array. A moving-window, cross-correlation analysis finds that these later arrivals are surface waves traveling from the south. The timing and propagation direction of these arrivals indicates that they were likely produced by scattering of incident S waves at the border of the Santa Clara Valley to the south of the array. It is remarkable that the largest low-frequency phases at many of the valley sites for regional events to the east are basin surface waves coming from a direction about 70 degrees different from that of the epicenters. Basin surface waves emanating from the eastern edge of the valley are also identified by the cross-correlation analysis.

Toward high-resolution optoelectronic retinal prosthesis

NASA Astrophysics Data System (ADS)

Palanker, Daniel; Huie, Philip; Vankov, Alexander; Asher, Alon; Baccus, Steven

2005-04-01

It has been already demonstrated that electrical stimulation of retina can produce visual percepts in blind patients suffering from macular degeneration and retinitis pigmentosa. Current retinal implants provide very low resolution (just a few electrodes), while several thousand pixels are required for functional restoration of sight. We present a design of the optoelectronic retinal prosthetic system that can activate a retinal stimulating array with pixel density up to 2,500 pix/mm2 (geometrically corresponding to a visual acuity of 20/80), and allows for natural eye scanning rather than scanning with a head-mounted camera. The system operates similarly to "virtual reality" imaging devices used in military and medical applications. An image from a video camera is projected by a goggle-mounted infrared LED-LCD display onto the retina, activating an array of powered photodiodes in the retinal implant. Such a system provides a broad field of vision by allowing for natural eye scanning. The goggles are transparent to visible light, thus allowing for simultaneous utilization of remaining natural vision along with prosthetic stimulation. Optical control of the implant allows for simple adjustment of image processing algorithms and for learning. A major prerequisite for high resolution stimulation is the proximity of neural cells to the stimulation sites. This can be achieved with sub-retinal implants constructed in a manner that directs migration of retinal cells to target areas. Two basic implant geometries are described: perforated membranes and protruding electrode arrays. Possibility of the tactile neural stimulation is also examined.

Electron-stimulated desorption from polished and vacuum fired 316LN stainless steel coated with Ti-Zr-Hf-V

DOE Office of Scientific and Technical Information (OSTI.GOV)

Malyshev, Oleg B., E-mail: oleg.malyshev@stfc.ac.uk; Valizadeh, Reza; Hogan, Benjamin T.

2014-11-01

In this study, two identical 316LN stainless steel tubular samples, which had previously been polished and vacuum-fired and then used for the electron-stimulated desorption (ESD) experiments, were coated with Ti-Zr-Hf-V with different morphologies: columnar and dense. ESD measurement results after nonevaporable getter (NEG) activation to 150, 180, 250, and 350 °C indicated that the values for the ESD yields are significantly (2–20 times) lower than the data from our previous study with similar coatings on nonvacuum-fired samples. Based on these results, the lowest pressure and best long-term performance in particle accelerators will be achieved with a vacuum-fired vacuum chamber coated withmore » dense Ti-Zr-Hf-V coating activated at 180 °C. This is likely due to the following facts: after NEG activation, the hydrogen concentration inside the NEG was lower than in the bulk stainless steel substrate; the NEG coating created a barrier for gas diffusion from the sample bulk to vacuum; the dense NEG coating performed better as a barrier than the columnar NEG coating.« less

Large-scale fabrication of vertically aligned ZnO nanowire arrays

DOEpatents

Wang, Zhong Lin; Hu, Youfan; Zhang, Yan; Xu, Chen; Zhu, Guang

2014-09-09

A generator includes a substrate, a first electrode layer, a dense plurality of vertically-aligned piezoelectric elongated nanostructures, an insulating layer and a second electrode layer. The substrate has a top surface and the first electrode layer is disposed on the top surface of the substrate. The dense plurality of vertically-aligned piezoelectric elongated nanostructures extends from the first electrode layer. Each of the nanostructures has a top end. The insulating layer is disposed on the top ends of the nanostructures. The second electrode layer is disposed on the non-conductive layer and is spaced apart from the nanostructures.

Simulation Study of an Ultrasound Retinal Prosthesis With a Novel Contact-Lens Array for Noninvasive Retinal Stimulation.

PubMed

Gao, Mengdi; Yu, Yanyan; Zhao, Huixia; Li, Guofeng; Jiang, Hongyang; Wang, Congzhi; Cai, Feiyan; Chan, Leanne Lai-Hang; Chiu, Bernard; Qian, Wei; Qiu, Weibao; Zheng, Hairong

2017-09-01

Millions of people around the world suffer from varying degrees of vision loss (including complete blindness) because of retinal degenerative diseases. Artificial retinal prosthesis, which is usually based on electrical neurostimulation, is the most advanced technology for different types of retinal degeneration. However, this technology involves placing a device into the eyeball, and such a highly invasive procedure is inevitably highly risk and expensive. Ultrasound has been demonstrated to be a promising technology for noninvasive neurostimulation, making it possible to stimulate the retina and induce action potentials similar to those elicited by light stimulation. However, the technology of ultrasound retinal stimulation still requires considerable developments before it could be applied clinically. This paper proposes a novel contact-lens array transducer for use in an ultrasound retinal prosthesis (USRP). The transducer was designed in the shape of a contact lens so as to facilitate acoustic coupling with the eye liquid. The key parameters of the ultrasound transducer were simulated, and results are presented that indicate the achievement of 2-D pattern generation and that the proposed contact-lens array is suitable for multiple-focus neurostimulation, and can be used in a USRP.

Noninvasive Assessment of Intracranial Pressure Status in Idiopathic Intracranial Hypertension Using Displacement Encoding with Stimulated Echoes (DENSE) MRI: A Prospective Patient Study with Contemporaneous CSF Pressure Correlation.

PubMed

Saindane, A M; Qiu, D; Oshinski, J N; Newman, N J; Biousse, V; Bruce, B B; Holbrook, J F; Dale, B M; Zhong, X

2018-02-01

Intracranial pressure is estimated invasively by using lumbar puncture with CSF opening pressure measurement. This study evaluated displacement encoding with stimulated echoes (DENSE), an MR imaging technique highly sensitive to brain motion, as a noninvasive means of assessing intracranial pressure status. Nine patients with suspected elevated intracranial pressure and 9 healthy control subjects were included in this prospective study. Controls underwent DENSE MR imaging through the midsagittal brain. Patients underwent DENSE MR imaging followed immediately by lumbar puncture with opening pressure measurement, CSF removal, closing pressure measurement, and immediate repeat DENSE MR imaging. Phase-reconstructed images were processed producing displacement maps, and pontine displacement was calculated. Patient data were analyzed to determine the effects of measured pressure on pontine displacement. Patient and control data were analyzed to assess the effects of clinical status (pre-lumbar puncture, post-lumbar puncture, or control) on pontine displacement. Patients demonstrated imaging findings suggesting chronically elevated intracranial pressure, whereas healthy control volunteers demonstrated no imaging abnormalities. All patients had elevated opening pressure (median, 36.0 cm water), decreased by the removal of CSF to a median closing pressure of 17.0 cm water. Patients pre-lumbar puncture had significantly smaller pontine displacement than they did post-lumbar puncture after CSF pressure reduction ( P = .001) and compared with controls ( P = .01). Post-lumbar puncture patients had statistically similar pontine displacements to controls. Measured CSF pressure in patients pre- and post-lumbar puncture correlated significantly with pontine displacement ( r = 0.49; P = .04). This study establishes a relationship between pontine displacement from DENSE MR imaging and measured pressure obtained contemporaneously by lumbar puncture, providing a method to noninvasively assess intracranial pressure status in idiopathic intracranial hypertension. © 2018 by American Journal of Neuroradiology.

Chronic intracortical microstimulation (ICMS) of cat sensory cortex using the Utah Intracortical Electrode Array.

PubMed

Rousche, P J; Normann, R A

1999-03-01

In an effort to assess the safety and efficacy of focal intracortical microstimulation (ICMS) of cerebral cortex with an array of penetrating electrodes as might be applied to a neuroprosthetic device to aid the deaf or blind, we have chronically implanted three trained cats in primary auditory cortex with the 100-electrode Utah Intracortical Electrode Array (UIEA). Eleven of the 100 electrodes were hard-wired to a percutaneous connector for chronic access. Prior to implant, cats were trained to "lever-press" in response to pure tone auditory stimulation. After implant, this behavior was transferred to "lever-presses" in response to current injections via single electrodes of the implanted arrays. Psychometric function curves relating injected charge level to the probability of response were obtained for stimulation of 22 separate electrodes in the three implanted cats. The average threshold charge/phase required for electrical stimulus detection in each cat was, 8.5, 8.6, and 11.6 nC/phase respectively, with a maximum charge/phase of 26 nC/phase and a minimum of 1.5 nC/phase thresholds were tracked for varying time intervals, and seven electrodes from two cats were tracked for up to 100 days. Electrodes were stimulated for no more than a few minutes each day. Neural recordings taken from the same electrodes before and after multiple electrical stimulation sessions were very similar in signal/noise ratio and in the number of recordable units, suggesting that the range of electrical stimulation levels used did not damage neurons in the vicinity of the electrodes. Although a few early implants failed, we conclude that ICMS of cerebral cortex to evoke a behavioral response can be achieved with the penetrating UIEA. Further experiments in support of a sensory cortical prosthesis based on ICMS are warranted.

Cortical activation following chronic passive implantation of a wide-field suprachoroidal retinal prosthesis

NASA Astrophysics Data System (ADS)

Villalobos, Joel; Fallon, James B.; Nayagam, David A. X.; Shivdasani, Mohit N.; Luu, Chi D.; Allen, Penelope J.; Shepherd, Robert K.; Williams, Chris E.

2014-08-01

Objective. The research goal is to develop a wide-field retinal stimulating array for prosthetic vision. This study aimed at evaluating the efficacy of a suprachoroidal electrode array in evoking visual cortex activity after long term implantation. Approach. A planar silicone based electrode array (8 mm × 19 mm) was implanted into the suprachoroidal space in cats (ntotal = 10). It consisted of 20 platinum stimulating electrodes (600 μm diameter) and a trans-scleral cable terminated in a subcutaneous connector. Three months after implantation (nchronic = 6), or immediately after implantation (nacute = 4), an electrophysiological study was performed. Electrode total impedance was measured from voltage transients using 500 μs, 1 mA pulses. Electrically evoked potentials (EEPs) and multi-unit activity were recorded from the visual cortex in response to monopolar retinal stimulation. Dynamic range and cortical activation spread were calculated from the multi-unit recordings. Main results. The mean electrode total impedance in vivo following 3 months was 12.5 ± 0.3 kΩ. EEPs were recorded for 98% of the electrodes. The median evoked potential threshold was 150 nC (charge density 53 μC cm-2). The lowest stimulation thresholds were found proximal to the area centralis. Mean thresholds from multiunit activity were lower for chronic (181 ± 14 nC) compared to acute (322 ± 20 nC) electrodes (P < 0.001), but there was no difference in dynamic range or cortical activation spread. Significance. Suprachoroidal stimulation threshold was lower in chronic than acute implantation and was within safe charge limits for platinum. Electrode-tissue impedance following chronic implantation was higher, indicating the need for sufficient compliance voltage (e.g. 12.8 V for mean impedance, threshold and dynamic range). The wide-field suprachoroidal array reliably activated the retina after chronic implantation.

Large-Scale Precise Printing of Ultrathin Sol-Gel Oxide Dielectrics for Directly Patterned Solution-Processed Metal Oxide Transistor Arrays.

PubMed

Lee, Won-June; Park, Won-Tae; Park, Sungjun; Sung, Sujin; Noh, Yong-Young; Yoon, Myung-Han

2015-09-09

Ultrathin and dense metal oxide gate di-electric layers are reported by a simple printing of AlOx and HfOx sol-gel precursors. Large-area printed indium gallium zinc oxide (IGZO) thin-film transistor arrays, which exhibit mobilities >5 cm(2) V(-1) s(-1) and gate leakage current of 10(-9) A cm(-2) at a very low operation voltage of 2 V, are demonstrated by continuous simple bar-coated processes. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Optics and Nonlinear Buckling Mechanics in Large-Area, Highly Stretchable Arrays of Plasmonic Nanostructures.

PubMed

Gao, Li; Zhang, Yihui; Zhang, Hui; Doshay, Sage; Xie, Xu; Luo, Hongying; Shah, Deesha; Shi, Yan; Xu, Siyi; Fang, Hui; Fan, Jonathan A; Nordlander, Peter; Huang, Yonggang; Rogers, John A

2015-06-23

Large-scale, dense arrays of plasmonic nanodisks on low-modulus, high-elongation elastomeric substrates represent a class of tunable optical systems, with reversible ability to shift key optical resonances over a range of nearly 600 nm at near-infrared wavelengths. At the most extreme levels of mechanical deformation (strains >100%), nonlinear buckling processes transform initially planar arrays into three-dimensional configurations, in which the nanodisks rotate out of the plane to form linear arrays with "wavy" geometries. Analytical, finite-element, and finite-difference time-domain models capture not only the physics of these buckling processes, including all of the observed modes, but also the quantitative effects of these deformations on the plasmonic responses. The results have relevance to mechanically tunable optical systems, particularly to soft optical sensors that integrate on or in the human body.

Tailoring uniform gold nanoparticle arrays and nanoporous films for next-generation optoelectronic devices

NASA Astrophysics Data System (ADS)

Farid, Sidra; Kuljic, Rade; Poduri, Shripriya; Dutta, Mitra; Darling, Seth B.

2018-06-01

High-density arrays of gold nanodots and nanoholes on indium tin oxide (ITO)-coated glass surfaces are fabricated using a nanoporous template fabricated by the self-assembly of diblock copolymers of poly (styrene-block-methyl methacrylate) (PS-b-PMMA) structures. By balancing the interfacial interactions between the polymer blocks and the substrate using random copolymer, cylindrical block copolymer microdomains oriented perpendicular to the plane of the substrate have been obtained. Nanoporous PS films are created by selectively etching PMMA cylinders, a straightforward route to form highly ordered nanoscale porous films. Deposition of gold on the template followed by lift off and sonication leaves a highly dense array of gold nanodots. These materials can serve as templates for the vapor-liquid-solid (VLS) growth of semiconductor nanorod arrays for next generation hybrid optoelectronic applications.

Cluster Computing For Real Time Seismic Array Analysis.

NASA Astrophysics Data System (ADS)

Martini, M.; Giudicepietro, F.

A seismic array is an instrument composed by a dense distribution of seismic sen- sors that allow to measure the directional properties of the wavefield (slowness or wavenumber vector) radiated by a seismic source. Over the last years arrays have been widely used in different fields of seismological researches. In particular they are applied in the investigation of seismic sources on volcanoes where they can be suc- cessfully used for studying the volcanic microtremor and long period events which are critical for getting information on the volcanic systems evolution. For this reason arrays could be usefully employed for the volcanoes monitoring, however the huge amount of data produced by this type of instruments and the processing techniques which are quite time consuming limited their potentiality for this application. In order to favor a direct application of arrays techniques to continuous volcano monitoring we designed and built a small PC cluster able to near real time computing the kinematics properties of the wavefield (slowness or wavenumber vector) produced by local seis- mic source. The cluster is composed of 8 Intel Pentium-III bi-processors PC working at 550 MHz, and has 4 Gigabytes of RAM memory. It runs under Linux operating system. The developed analysis software package is based on the Multiple SIgnal Classification (MUSIC) algorithm and is written in Fortran. The message-passing part is based upon the LAM programming environment package, an open-source imple- mentation of the Message Passing Interface (MPI). The developed software system includes modules devote to receiving date by internet and graphical applications for the continuous displaying of the processing results. The system has been tested with a data set collected during a seismic experiment conducted on Etna in 1999 when two dense seismic arrays have been deployed on the northeast and the southeast flanks of this volcano. A real time continuous acquisition system has been simulated by a pro- gram which reads data from disk files and send them to a remote host by using the Internet protocols.

Amelogenin-assisted ex vivo remineralization of human enamel: effects of supersaturation degree and fluoride concentration

PubMed Central

Fan, Yuwei; Nelson, James R.; Alvarez, Jason R.; Hagan, Joseph; Berrier, Allison; Xu, Xiaoming

2011-01-01

The formation of organized nanocrystals that resemble enamel is crucial for successful enamel remineralization. Calcium, phosphate and fluoride ions and amelogenin are important ingredients for the formation of organized hydroxyapatite (HAP) crystals in vitro. However, the effects of these remineralization agents on the enamel crystal morphology have not been thoroughly studied. The objective of this study was to investigate the effects of fluoride ions, supersaturation degree and amelogenin on the crystal morphology and organization of ex vivo remineralized human enamel. Extracted third molars were sliced thin and acid-etched to provide the enamel surface for immersion in different remineralization solutions. The crystal morphology and mineral phase of the remineralized enamel surface were analyzed by FE-SEM, ATR-FTIR and XRD. The concentration of fluoride and supersaturation degree of hydroxyapatite had significant effects on the crystal morphology and crystal organization, which varied from plate-like loose crystals to rod-like densely packed nanocrystal arrays. Densely packed arrays of fluoridated hydroxyapatite nanorods were observed under the following conditions: σ(HAP) = 10.2±2.0 with fluoride 1.5±0.5 mg/L and amelogenin 40±10 µg/mL, pH 6.8±0.4. A phase diagram summarized the conditions that form dense or loose hydroxyapatite nanocrystal structures. This study provides the basis for the development of novel dental materials for caries management. PMID:21256987

En route to controlled catalytic CVD synthesis of densely packed and vertically aligned nitrogen-doped carbon nanotube arrays.

PubMed

Boncel, Slawomir; Pattinson, Sebastian W; Geiser, Valérie; Shaffer, Milo S P; Koziol, Krzysztof K K

2014-01-01

The catalytic chemical vapour deposition (c-CVD) technique was applied in the synthesis of vertically aligned arrays of nitrogen-doped carbon nanotubes (N-CNTs). A mixture of toluene (main carbon source), pyrazine (1,4-diazine, nitrogen source) and ferrocene (catalyst precursor) was used as the injection feedstock. To optimize conditions for growing the most dense and aligned N-CNT arrays, we investigated the influence of key parameters, i.e., growth temperature (660, 760 and 860 °C), composition of the feedstock and time of growth, on morphology and properties of N-CNTs. The presence of nitrogen species in the hot zone of the quartz reactor decreased the growth rate of N-CNTs down to about one twentieth compared to the growth rate of multi-wall CNTs (MWCNTs). As revealed by electron microscopy studies (SEM, TEM), the individual N-CNTs (half as thick as MWCNTs) grown under the optimal conditions were characterized by a superior straightness of the outer walls, which translated into a high alignment of dense nanotube arrays, i.e., 5 × 10(8) nanotubes per mm(2) (100 times more than for MWCNTs grown in the absence of nitrogen precursor). In turn, the internal crystallographic order of the N-CNTs was found to be of a 'bamboo'-like or 'membrane'-like (multi-compartmental structure) morphology. The nitrogen content in the nanotube products, which ranged from 0.0 to 3.0 wt %, was controlled through the concentration of pyrazine in the feedstock. Moreover, as revealed by Raman/FT-IR spectroscopy, the incorporation of nitrogen atoms into the nanotube walls was found to be proportional to the number of deviations from the sp(2)-hybridisation of graphene C-atoms. As studied by XRD, the temperature and the [pyrazine]/[ferrocene] ratio in the feedstock affected the composition of the catalyst particles, and hence changed the growth mechanism of individual N-CNTs into a 'mixed base-and-tip' (primarily of the base-type) type as compared to the purely 'base'-type for undoped MWCNTs.

[The effect of needleless electroacupuncture in general anesthesia during laparoscopic surgery].

PubMed

Chiang, M H; Wong, J O; Chang, D P; Dai, Y B; Chen, C C; Lee, S C; Chang, C L

1995-06-01

Three kinds of pain-relieving substances, namely, endorphins, enkephalins and dynorphins, can be released by stimulating the relevant acupoints with the dense-disperse mode of nerve stimulator. The neurochemical mechanisms of pain relief by acupoint stimulation have been widely studied and proved. In the present study, we investigated the modulatory effect of needleless electroacupuncture on the inhaled general anesthesia during laparoscopic surgery. Forty gynecologic patients of ASA class I-II status, scheduled for elective laparoscopic surgery, were randomly allocated to study and control groups. Induction and intubation were performed in the same fashion and anesthesia was maintained with inhaled general anesthetics: 50% N2O in oxygen and isoflurane, which was adjusted to keep the hemodynamic changes within +/- 10% of their preoperated level. HANS (LY 257), a special nerve stimulator with 2 Hz and 100 Hz dense-disperse wave, was used to stimulate the bilateral Yang Ling Chuan (G34), Zusanli (S36) acupoints in patients of the study group during the surgery. We found that needleless electroacupuncture significantly lowered the volume concentration of isoflurane from 1.0 +/- 0.33% to 0.74 +/- 0.19% (p < 0.05) at 30 min after the start of operation. Furthermore, it also significantly shortened the recovery time from 11.4 +/- 3.3 min to 8.8 +/- 3.2 min (p < 0.05). Under general anesthesia, the application of needleless electroacupuncture can reduce the volume concentration of isoflurane and shorten the post-anesthetic recovery time during laparoscopic surgery.

Developing and Evaluating a Flexible Wireless Microcoil Array Based Integrated Interface for Epidural Cortical Stimulation.

PubMed

Wang, Xing; Chaudhry, Sharjeel A; Hou, Wensheng; Jia, Xiaofeng

2017-02-05

Stroke leads to serious long-term disability. Electrical epidural cortical stimulation has made significant improvements in stroke rehabilitation therapy. We developed a preliminary wireless implantable passive interface, which consists of a stimulating surface electrode, receiving coil, and single flexible passive demodulated circuit printed by flexible printed circuit (FPC) technique and output pulse voltage stimulus by inductively coupling an external circuit. The wireless implantable board was implanted in cats' unilateral epidural space for electrical stimulation of the primary visual cortex (V1) while the evoked responses were recorded on the contralateral V1 using a needle electrode. The wireless implantable board output stable monophasic voltage stimuli. The amplitude of the monophasic voltage output could be adjusted by controlling the voltage of the transmitter circuit within a range of 5-20 V. In acute experiment, cortico-cortical evoked potential (CCEP) response was recorded on the contralateral V1. The amplitude of N2 in CCEP was modulated by adjusting the stimulation intensity of the wireless interface. These results demonstrated that a wireless interface based on a microcoil array can offer a valuable tool for researchers to explore electrical stimulation in research and the dura mater-electrode interface can effectively transmit electrical stimulation.

Organization and mobility of CD11b/CD18 and targeting of superoxide on the surface of degranulated human neutrophils.

PubMed

Mukherjee, G; Rasmusson, B; Linner, J G; Quinn, M T; Parkos, C A; Magnusson, K E; Jesaitis, A J

1998-09-01

A monoclonal IgM, specifically recognizing both CD11b and CD18 of human neutrophils, was used to examine the organization and mobility of CD11b/CD18 in the plasma membrane of human neutrophils degranulated by dihydrocytochalasin B (dhCB) treatment and fMet-Leu-Phe (fMLF) stimulation. Subcellular fractionation analysis of untreated or dhCB-treated control neutrophils indicated that 20% of CD11b/CD18 cosedimented with plasma membrane and the remainder with specific granules. In contrast, fMLF stimulation of dhCB-treated cells caused a major reorganization of CD11b/CD18, in which 60-70% of CD11b/CD18 sedimented in dense plasma membrane fractions that were also enriched in superoxide-generating NADPH oxidase activity. Similarly pretreated neutrophils were fixed, immunogold labeled, and examined by scanning electron microscopy. Immunogold particles were distributed uniformly over the symmetrically ruffled surface of unstimulated neutrophils. On dhCB-treated cells, immunogold was mostly uniformly distributed on a smooth membrane with a small percentage of particles lining up into linear arrays. After fMLF + dhCB stimulation, CD11b/CD18 gold label was more abundant on the cell surface and formed large aggregates on polarized membrane protrusions. However, when cells were adhered to an albumin-coated quartz surface and stimulated with fMLF in the presence of dhCB, immunogold was excluded on the articulated and rounded cell body but concentrated on the periphery of adherent lamellae. Fluorescence photobleaching recovery indicated that in unstimulated cells 38 +/- 3% of CD11b/CD18 was mobile (R) with a diffusion constant D of 3.1 +/- 0.3 x 10(-10) cm2/s. Treatment with dhCB raised R and D 24 and 74%, respectively. Stimulation using 1 microM fMLF with dhCB lowered D and R to near control levels. Since NADPH oxidase and CD11b/CD18 cosediment in high-density plasma membrane domains after fMLF + dhCB stimulation, we speculate that a stimulus-induced reorganization of CD11b/CD18 and NADPH oxidase to common membrane domains may occur in fMLF + dhCB-degranulated neutrophils. Copyright 1998 Academic Press.

Hierarchical Branched Vanadium Oxide Nanorod@Si Nanowire Architecture for High Performance Supercapacitors.

PubMed

Li, Zhaodong; Wang, Fei; Wang, Xudong

2017-01-01

Vanadium oxide (VO x ) nanorods are uniformly synthesized on dense Si nanowire arrays. This 3D hierarchical nanoarchitecture offers a novel high-performance supercapacitor electrode design with significantly improved specific capacitance and high-rate capability. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

A Multi-Walled Carbon Nanotube-based Biosensor for Monitoring Microcystin-LR in Sources of Drinking Water Supplies

EPA Science Inventory

A multi-walled carbon nanotube-based electrochemical biosensor is developed for monitoring microcystin-LR (MC-LR), a toxic cyanobacterial toxin, in sources of drinking water supplies. The biosensor electrodes are fabricated using dense, mm-long multi-walled CNT (MWCNT) arrays gro...

Low cost sensors for PM and related air pollutants in the US and India

EPA Science Inventory

Emerging air quality sensors have a variety of possible applications. If accurate and reliable, they have a number of benefits over conventional monitors. They are low-cost, lightweight, and have low power consumption. Because of their low cost, a dense array of sensors instal...

The Molecular Gas Environment in the 20 km s{sup −1} Cloud in the Central Molecular Zone

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lu, Xing; Gu, Qiusheng; Zhang, Qizhou

We recently reported a population of protostellar candidates in the 20 km s{sup −1} cloud in the Central Molecular Zone of the Milky Way, traced by H{sub 2}O masers in gravitationally bound dense cores. In this paper, we report molecular line studies with high angular resolution (∼3″) of the environment of star formation in this cloud. Maps of various molecular line transitions as well as the continuum at 1.3 mm are obtained using the Submillimeter Array. Five NH{sub 3} inversion lines and the 1.3 cm continuum are observed with the Karl G. Jansky Very Large Array. The interferometric observations aremore » complemented with single-dish data. We find that the CH{sub 3}OH, SO, and HNCO lines, which are usually shock tracers, are better correlated spatially with the compact dust emission from dense cores among the detected lines. These lines also show enhancement in intensities with respect to SiO intensities toward the compact dust emission, suggesting the presence of slow shocks or hot cores in these regions. We find gas temperatures of ≳100 K at 0.1 pc scales based on RADEX modeling of the H{sub 2}CO and NH{sub 3} lines. Although no strong correlations between temperatures and linewidths/H{sub 2}O maser luminosities are found, in high-angular-resolution maps we note several candidate shock-heated regions offset from any dense cores, as well as signatures of localized heating by protostars in several dense cores. Our findings suggest that at 0.1 pc scales in this cloud star formation and strong turbulence may together affect the chemistry and temperature of the molecular gas.« less

Mechanical Design and Development of TES Bolometer Detector Arrays for the Advanced ACTPol Experiment

NASA Technical Reports Server (NTRS)

Ward, Jonathan T.; Austermann, Jason; Beall, James A.; Choi, Steve K.; Crowley, Kevin T.; Devlin, Mark J.; Duff, Shannon M.; Gallardo, Patricio M.; Henderson, Shawn W.; Ho, Shuay-Pwu Patty;

Mechanical designs and development of TES bolometer detector arrays for the Advanced ACTPol experiment

NASA Astrophysics Data System (ADS)

Ward, Jonathan T.; Austermann, Jason; Beall, James A.; Choi, Steve K.; Crowley, Kevin T.; Devlin, Mark J.; Duff, Shannon M.; Gallardo, Patricio A.; Henderson, Shawn W.; Ho, Shuay-Pwu Patty; Hilton, Gene; Hubmayr, Johannes; Khavari, Niloufar; Klein, Jeffrey; Koopman, Brian J.; Li, Dale; McMahon, Jeffrey; Mumby, Grace; Nati, Federico; Niemack, Michael D.; Page, Lyman A.; Salatino, Maria; Schillaci, Alessandro; Schmitt, Benjamin L.; Simon, Sara M.; Staggs, Suzanne T.; Thornton, Robert; Ullom, Joel N.; Vavagiakis, Eve M.; Wollack, Edward J.

2016-07-01

The next generation Advanced ACTPol (AdvACT) experiment is currently underway and will consist of four Transition Edge Sensor (TES) bolometer arrays, with three operating together, totaling 5800 detectors on the sky. Building on experience gained with the ACTPol detector arrays, AdvACT will utilize various new technologies, including 150 mm detector wafers equipped with multichroic pixels, allowing for a more densely packed focal plane. Each set of detectors includes a feedhorn array of stacked silicon wafers which form a spline profile leading to each pixel. This is then followed by a waveguide interface plate, detector wafer, back short cavity plate, and backshort cap. Each array is housed in a custom designed structure manufactured from high purity copper and then gold plated. In addition to the detector array assembly, the array package also encloses cryogenic readout electronics. We present the full mechanical design of the AdvACT high frequency (HF) detector array package along with a detailed look at the detector array stack assemblies. This experiment will also make use of extensive hardware and software previously developed for ACT, which will be modified to incorporate the new AdvACT instruments. Therefore, we discuss the integration of all AdvACT arrays with pre-existing ACTPol infrastructure.

Investigating brain functional evolution and plasticity using microelectrode array technology.

PubMed

Napoli, Alessandro; Obeid, Iyad

2015-10-01

The aim of this work was to investigate long and short-term plasticity responsible for memory formation in dissociated neuronal networks. In order to address this issue, a set of experiments was designed and implemented in which the microelectrode array electrode grid was divided into four quadrants, two of which were chronically stimulated, every two days for one hour with a stimulation paradigm that varied over time. Overall network and quadrant responses were then analyzed to quantify what level of plasticity took place in the network and how this was due to the stimulation interruption. The results demonstrate that there were no spatial differences in the stimulus-evoked activity within quadrants. Furthermore, the implemented stimulation protocol induced depression effects in the neuronal networks as demonstrated by the consistently lower network activity following stimulation sessions. Finally, the analysis demonstrated that the inhibitory effects of the stimulation decreased over time, thus suggesting a habituation phenomenon. These findings are sufficient to conclude that electrical stimulation is an important tool to interact with dissociated neuronal cultures, but localized stimuli are not enough to drive spatial synaptic potentiation or depression. On the contrary, the ability to modulate synaptic temporal plasticity was a feasible task to achieve by chronic network stimulation. Copyright © 2015 Elsevier Inc. All rights reserved.

Characteristics of electrode impedance and stimulation efficacy of a chronic cortical implant using novel annulus electrodes in rat motor cortex

NASA Astrophysics Data System (ADS)

Wang, Chun; Brunton, Emma; Haghgooie, Saman; Cassells, Kahli; Lowery, Arthur; Rajan, Ramesh

2013-08-01

Objective. Cortical neural prostheses with implanted electrode arrays have been used to restore compromised brain functions but concerns remain regarding their long-term stability and functional performance. Approach. Here we report changes in electrode impedance and stimulation thresholds for a custom-designed electrode array implanted in rat motor cortex for up to three months. Main Results. The array comprises four 2000 µm long electrodes with a large annular stimulating surface (7860-15700 µm2) displaced from the penetrating insulated tip. Compared to pre-implantation in vitro values there were three phases of impedance change: (1) an immediate large increase of impedance by an average of two-fold on implantation; (2) a period of continued impedance increase, albeit with considerable variability, which reached a peak at approximately four weeks post-implantation and remained high over the next two weeks; (3) finally, a period of 5-6 weeks when impedance stabilized at levels close to those seen immediately post-implantation. Impedance could often be temporarily decreased by applying brief trains of current stimulation, used to evoke motor output. The stimulation threshold to induce observable motor behaviour was generally between 75-100 µA, with charge density varying from 48-128 µC cm-2, consistent with the lower current density generated by electrodes with larger stimulating surface area. No systematic change in thresholds occurred over time, suggesting that device functionality was not compromised by the factors that caused changes in electrode impedance. Significance. The present results provide support for the use of annulus electrodes in future applications in cortical neural prostheses.

Optogenetically induced spatiotemporal gamma oscillations and neuronal spiking activity in primate motor cortex.

PubMed

Lu, Yao; Truccolo, Wilson; Wagner, Fabien B; Vargas-Irwin, Carlos E; Ozden, Ilker; Zimmermann, Jonas B; May, Travis; Agha, Naubahar S; Wang, Jing; Nurmikko, Arto V

2015-06-01

Transient gamma-band (40-80 Hz) spatiotemporal patterns are hypothesized to play important roles in cortical function. Here we report the direct observation of gamma oscillations as spatiotemporal waves induced by targeted optogenetic stimulation, recorded by intracortical multichannel extracellular techniques in macaque monkeys during their awake resting states. Microelectrode arrays integrating an optical fiber at their center were chronically implanted in primary motor (M1) and ventral premotor (PMv) cortices of two subjects. Targeted brain tissue was transduced with the red-shifted opsin C1V1(T/T). Constant (1-s square pulses) and ramp stimulation induced narrowband gamma oscillations during awake resting states. Recordings across 95 microelectrodes (4 × 4-mm array) enabled us to track the transient gamma spatiotemporal patterns manifested, e.g., as concentric expanding and spiral waves. Gamma oscillations were induced well beyond the light stimulation volume, via network interactions at distal electrode sites, depending on optical power. Despite stimulation-related modulation in spiking rates, neuronal spiking remained highly asynchronous during induced gamma oscillations. In one subject we examined stimulation effects during preparation and execution of a motor task and observed that movement execution largely attenuated optically induced gamma oscillations. Our findings demonstrate that, beyond previously reported induced gamma activity under periodic drive, a prolonged constant stimulus above a certain threshold may carry primate motor cortex network dynamics into gamma oscillations, likely via a Hopf bifurcation. More broadly, the experimental capability in combining microelectrode array recordings and optogenetic stimulation provides an important approach for probing spatiotemporal dynamics in primate cortical networks during various physiological and behavioral conditions.

Optogenetically induced spatiotemporal gamma oscillations and neuronal spiking activity in primate motor cortex

PubMed Central

Lu, Yao; Truccolo, Wilson; Wagner, Fabien B.; Vargas-Irwin, Carlos E.; Ozden, Ilker; Zimmermann, Jonas B.; May, Travis; Agha, Naubahar S.; Wang, Jing

2015-01-01

Transient gamma-band (40–80 Hz) spatiotemporal patterns are hypothesized to play important roles in cortical function. Here we report the direct observation of gamma oscillations as spatiotemporal waves induced by targeted optogenetic stimulation, recorded by intracortical multichannel extracellular techniques in macaque monkeys during their awake resting states. Microelectrode arrays integrating an optical fiber at their center were chronically implanted in primary motor (M1) and ventral premotor (PMv) cortices of two subjects. Targeted brain tissue was transduced with the red-shifted opsin C1V1(T/T). Constant (1-s square pulses) and ramp stimulation induced narrowband gamma oscillations during awake resting states. Recordings across 95 microelectrodes (4 × 4-mm array) enabled us to track the transient gamma spatiotemporal patterns manifested, e.g., as concentric expanding and spiral waves. Gamma oscillations were induced well beyond the light stimulation volume, via network interactions at distal electrode sites, depending on optical power. Despite stimulation-related modulation in spiking rates, neuronal spiking remained highly asynchronous during induced gamma oscillations. In one subject we examined stimulation effects during preparation and execution of a motor task and observed that movement execution largely attenuated optically induced gamma oscillations. Our findings demonstrate that, beyond previously reported induced gamma activity under periodic drive, a prolonged constant stimulus above a certain threshold may carry primate motor cortex network dynamics into gamma oscillations, likely via a Hopf bifurcation. More broadly, the experimental capability in combining microelectrode array recordings and optogenetic stimulation provides an important approach for probing spatiotemporal dynamics in primate cortical networks during various physiological and behavioral conditions. PMID:25761956

Clogging and jamming transitions in periodic obstacle arrays

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nguyen, Hong; Reichhardt, Charles; Olson Reichhardt, Cynthia Jane

2017-03-29

We numerically examine clogging transitions for bidisperse disks flowing through a two-dimensional periodic obstacle array. Here, we show that clogging is a probabilistic event that occurs through a transition from a homogeneous flowing state to a heterogeneous or phase-separated jammed state where the disks form dense connected clusters. The probability for clogging to occur during a fixed time increases with increasing particle packing and obstacle number. For driving at different angles with respect to the symmetry direction of the obstacle array, we show that certain directions have a higher clogging susceptibility. It is also possible to have a size-specific cloggingmore » transition in which one disk size becomes completely immobile while the other disk size continues to flow.« less

Human eye-inspired soft optoelectronic device using high-density MoS2-graphene curved image sensor array.

PubMed

Choi, Changsoon; Choi, Moon Kee; Liu, Siyi; Kim, Min Sung; Park, Ok Kyu; Im, Changkyun; Kim, Jaemin; Qin, Xiaoliang; Lee, Gil Ju; Cho, Kyoung Won; Kim, Myungbin; Joh, Eehyung; Lee, Jongha; Son, Donghee; Kwon, Seung-Hae; Jeon, Noo Li; Song, Young Min; Lu, Nanshu; Kim, Dae-Hyeong

2017-11-21

Soft bioelectronic devices provide new opportunities for next-generation implantable devices owing to their soft mechanical nature that leads to minimal tissue damages and immune responses. However, a soft form of the implantable optoelectronic device for optical sensing and retinal stimulation has not been developed yet because of the bulkiness and rigidity of conventional imaging modules and their composing materials. Here, we describe a high-density and hemispherically curved image sensor array that leverages the atomically thin MoS 2 -graphene heterostructure and strain-releasing device designs. The hemispherically curved image sensor array exhibits infrared blindness and successfully acquires pixelated optical signals. We corroborate the validity of the proposed soft materials and ultrathin device designs through theoretical modeling and finite element analysis. Then, we propose the ultrathin hemispherically curved image sensor array as a promising imaging element in the soft retinal implant. The CurvIS array is applied as a human eye-inspired soft implantable optoelectronic device that can detect optical signals and apply programmed electrical stimulation to optic nerves with minimum mechanical side effects to the retina.

THE GREEN BANK TELESCOPE MAPS THE DENSE, STAR-FORMING GAS IN THE NEARBY STARBURST GALAXY M82

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kepley, Amanda A.; Frayer, David; Leroy, Adam K.

Observations of the Milky Way and nearby galaxies show that dense molecular gas correlates with recent star formation, suggesting that the formation of this gas phase may help regulate star formation. A key test of this idea requires wide-area, high-resolution maps of dense molecular gas in galaxies to explore how local physical conditions drive dense gas formation, but these observations have been limited because of the faintness of dense gas tracers like HCN and HCO{sup +}. Here we demonstrate the power of the Robert C. Byrd Green Bank Telescope (GBT)—the largest single-dish millimeter radio telescope—for mapping dense gas in galaxiesmore » by presenting the most sensitive maps yet of HCN and HCO{sup +} in the starburst galaxy M82. The HCN and HCO{sup +} in the disk of this galaxy correlates with both recent star formation and more diffuse molecular gas and shows kinematics consistent with a rotating torus. The HCO{sup +} emission extending to the north and south of the disk is coincident with the outflow previously identified in CO and traces the eastern edge of the hot outflowing gas. The central starburst region has a higher ratio of star formation to dense gas than the outer regions, pointing to the starburst as a key driver of this relationship. These results establish that the GBT can efficiently map the dense molecular gas at 90 GHz in nearby galaxies, a capability that will increase further with the 16 element feed array under construction.« less

Temporal-Spectral Characterization and Classification of Marine Mammal Vocalizations and Diesel-Electric Ships Radiated Sound over Continental Shelf Scale Regions with Coherent Hydrophone Array Measurements

NASA Astrophysics Data System (ADS)

Huang, Wei

The passive ocean acoustic waveguide remote sensing (POAWRS) technology is capable of monitoring a large variety of underwater sound sources over instantaneous wide areas spanning continental-shelf scale regions. POAWRS uses a large-aperture densely-sampled coherent hydrophone array to significantly enhance the signal-to-noise ratio via beamforming, enabling detection of sound sources roughly two-orders of magnitude more distant in range than that possible with a single hydrophone. The sound sources detected by POAWRS include ocean biology, geophysical processes, and man-made activities. POAWRS provides detection, bearing-time estimation, localization, and classification of underwater sound sources. The volume of underwater sounds detected by POAWRS is immense, typically exceeding a million unique signal detections per day, in the 10-4000 Hz frequency range, making it a tremendously challenging task to distinguish and categorize the various sound sources present in a given region. Here we develop various approaches for characterizing and clustering the signal detections for various subsets of data acquired using the POAWRS technology. The approaches include pitch tracking of the dominant signal detections, time-frequency feature extraction, clustering and categorization methods. These approaches are essential for automatic processing and enhancing the efficiency and accuracy of POAWRS data analysis. The results of the signal detection, clustering and classification analysis are required for further POAWRS processing, including localization and tracking of a large number of oceanic sound sources. Here the POAWRS detection, localization and clustering approaches are applied to analyze and elucidate the vocalization behavior of humpback, sperm and fin whales in the New England continental shelf and slope, including the Gulf of Maine from data acquired using coherent hydrophone arrays. The POAWRS technology can also be applied for monitoring ocean vehicles. Here the approach is calibrated by application to known ships present in the Gulf of Maine and in the Norwegian Sea from their underwater sounds received using a coherent hydrophone array. The vocalization behavior of humpback whales was monitored over vast areas of the Gulf of Maine using the POAWRS technique over multiple diel cycles in Fall 2006. The humpback vocalizations, received at a rate of roughly 1800+/-1100 calls per day, comprised of both song and non-song. The song vocalizations, composed of highly structured and repeatable set of phrases, are characterized by inter-pulse intervals of 3.5 +/- 1.8 s. Songs were detected throughout the diel cycle, occuring roughly 40% during the day and 60% during the night. The humpback non-song vocalizations, dominated by shorter duration (≤3 s) downsweep and bow-shaped moans, as well as a small fraction of longer duration (˜5 s) cries, have significantly larger mean and more variable inter-pulse intervals of 14.2 +/- 11 s. The non-song vocalizations were detected at night with negligible detections during the day, implying they probably function as nighttime communication signals. The humpback song and non-song vocalizations are separately localized using the moving array triangulation and array invariant techniques. The humpback song and non-song moan calls are both consistently localized to a dense area on northeastern Georges Bank and a less dense region extended from Franklin Basin to the Great South Channel. Humpback cries occur exclusively on northeastern Georges Bank and during nights with coincident dense Atlantic herring shoaling populations, implying the cries are feeding-related. Sperm whales in the New England continental shelf and slope were passively localized and classified from their vocalizations received using a single low-frequency (<2500 Hz) densely-sampled horizontal coherent hydrophone array deployed in Spring 2013 in Gulf of Maine. Whale bearings were estimated using time-domain beamforming that provided high coherent array gain in sperm whale click signal-to-noise ratio. Whale ranges from the receiver array center were estimated using the moving array triangulation technique from a sequence of whale bearing measurements. Multiple concurrently vocalizing sperm whales, in the far-field of the horizontal receiver array, were distinguished and classified based on their horizontal spatial locations and the inter-pulse intervals of their vocalized click signals. We provide detailed analysis of over 15,000 fin whale 20 Hz vocalizations received on Oct 1-3, 2006 in the Gulf of Maine. These vocalizations are separated into 16 clusters following the clustering approaches. Seven of these types are prominent, each acounting for between 8% to 16% and together comprise roughly 85% of all the analyzed vocalizations. The 7 prominent clusters are each more abundant during nighttime hours by a factor of roughly 2.5 times than that of the daytime. The diel-spatial correlation of the 7 prominent clusters to the simultaneously observed densities of their fish prey, the Atlantic herring in the Gulf of Maine, is provided which implies that the factor of roughly 2.5 increase in call rate during night-time hours can be attributed to increased fish-feeding activities. (Abstract shortened by ProQuest.).

Carbon-Nanotube-Based Electrodes for Biomedical Applications

NASA Technical Reports Server (NTRS)

Li, Jun; Meyyappan, M.

2008-01-01

A nanotube array based on vertically aligned nanotubes or carbon nanofibers has been invented for use in localized electrical stimulation and recording of electrical responses in selected regions of an animal body, especially including the brain. There are numerous established, emerging, and potential applications for localized electrical stimulation and/or recording, including treatment of Parkinson s disease, Tourette s syndrome, and chronic pain, and research on electrochemical effects involved in neurotransmission. Carbon-nanotube-based electrodes offer potential advantages over metal macroelectrodes (having diameters of the order of a millimeter) and microelectrodes (having various diameters ranging down to tens of microns) heretofore used in such applications. These advantages include the following: a) Stimuli and responses could be localized at finer scales of spatial and temporal resolution, which is at subcellular level, with fewer disturbances to, and less interference from, adjacent regions. b) There would be less risk of hemorrhage on implantation because nano-electrode-based probe tips could be configured to be less traumatic. c) Being more biocompatible than are metal electrodes, carbon-nanotube-based electrodes and arrays would be more suitable for long-term or permanent implantation. d) Unlike macro- and microelectrodes, a nano-electrode could penetrate a cell membrane with minimal disruption. Thus, for example, a nanoelectrode could be used to generate an action potential inside a neuron or in proximity of an active neuron zone. Such stimulation may be much more effective than is extra- or intracellular stimulation via a macro- or microelectrode. e) The large surface area of an array at a micron-scale footprint of non-insulated nanoelectrodes coated with a suitable electrochemically active material containing redox ingredients would make it possible to obtain a pseudocapacitance large enough to dissipate a relatively large amount of electric charge, so that a large stimulation current could be applied at a micron-scale region without exhausting the redox ingredients. f) Carbon nanotube array is more compatible with the three-dimensional network of tissues. Particularly, a better electrical-neural interface can be formed. g) A carbon nanotube array inlaid in insulating materials with only the ends exposed is an extremely sensitive electro-analysis tool that can measure the local neurotransmitter signal at extremely high sensitivity and temporal resolution.

Argus: A W-band 16-pixel focal plane array for the Green Bank Telescope

NASA Astrophysics Data System (ADS)

Devaraj, Kiruthika; Church, Sarah; Cleary, Kieran; Frayer, David; Gawande, Rohit; Goldsmith, Paul; Gundersen, Joshua; Harris, Andrew; Kangaslahti, Pekka; Readhead, Tony; Reeves, Rodrigo; Samoska, Lorene; Sieth, Matt; Voll, Patricia

2015-05-01

We are building Argus, a 16-pixel square-packed focal plane array that will cover the 75-115.3 GHz frequency range on the Robert C. Byrd Green Bank Telescope (GBT). The primary research area for Argus is the study of star formation within our Galaxy and nearby galaxies. Argus will map key molecules that trace star formation, including carbon monoxide (CO) and hydrogen cyanide (HCN). An additional key science area is astrochemistry, which will be addressed by observing complex molecules in the interstellar medium, and the study of formation of solar systems, which will be addressed by identifying dense pre-stellar cores and by observing comets in our solar system. Argus has a highly scalable architecture and will be a technology path finder for larger arrays. The array is modular in construction, which will allow easy replacement of malfunctioning and poorly performing components.

MEMS-Based Solid Propellant Rocket Array Thruster

NASA Astrophysics Data System (ADS)

Tanaka, Shuji; Hosokawa, Ryuichiro; Tokudome, Shin-Ichiro; Hori, Keiichi; Saito, Hirobumi; Watanabe, Masashi; Esashi, Masayoshi

The prototype of a solid propellant rocket array thruster for simple attitude control of a 10 kg class micro-spacecraft was completed and tested. The prototype has 10×10 φ0.8 mm solid propellant micro-rockets arrayed at a pitch of 1.2 mm on a 20×22 mm substrate. To realize such a dense array of micro-rockets, each ignition heater is powered from the backside of the thruster through an electrical feedthrough which passes along a propellant cylinder wall. Boron/potassium nitrate propellant (NAB) is used with/without lead rhodanide/potassium chlorate/nitrocellulose ignition aid (RK). Impulse thrust was measured by a pendulum method in air. Ignition required electric power of at least 3 4 W with RK and 4 6 W without RK. Measured impulse thrusts were from 2×10-5 Ns to 3×10-4 Ns after the calculation of compensation for air dumping.

A review of the design and clinical evaluation of the ShefStim array-based functional electrical stimulation system.

PubMed

Kenney, Laurence P; Heller, Ben W; Barker, Anthony T; Reeves, Mark L; Healey, Jamie; Good, Timothy R; Cooper, Glen; Sha, Ning; Prenton, Sarah; Liu, Anmin; Howard, David

2016-11-01

Functional electrical stimulation has been shown to be a safe and effective means of correcting foot drop of central neurological origin. Current surface-based devices typically consist of a single channel stimulator, a sensor for determining gait phase and a cuff, within which is housed the anode and cathode. The cuff-mounted electrode design reduces the likelihood of large errors in electrode placement, but the user is still fully responsible for selecting the correct stimulation level each time the system is donned. Researchers have investigated different approaches to automating aspects of setup and/or use, including recent promising work based on iterative learning techniques. This paper reports on the design and clinical evaluation of an electrode array-based FES system for the correction of drop foot, ShefStim. The paper reviews the design process from proof of concept lab-based study, through modelling of the array geometry and interface layer to array search algorithm development. Finally, the paper summarises two clinical studies involving patients with drop foot. The results suggest that the ShefStim system with automated setup produces results which are comparable with clinician setup of conventional systems. Further, the final study demonstrated that patients can use the system without clinical supervision. When used unsupervised, setup time was 14min (9min for automated search plus 5min for donning the equipment), although this figure could be reduced significantly with relatively minor changes to the design. Copyright © 2016 IPEM. Published by Elsevier Ltd. All rights reserved.

Optical design of GaN nanowire arrays for photocatalytic applications

NASA Astrophysics Data System (ADS)

Winnerl, Julia; Hudeczek, Richard; Stutzmann, Martin

2018-05-01

GaN nanowire (NW) arrays are interesting candidates for photocatalytic applications due to their high surface-to-volume ratio and their waveguide character. The integration of GaN NW arrays on GaN-based light emitting diodes (LEDs), serving as a platform for electrically driven NW-based photocatalytic devices, enables an efficient coupling of the light from the planar LED to the GaN NWs. Here, we present a numerical study of the influence of the NW geometries, i.e., the NW diameter, length, and period, and the illumination wavelength on the transmission of GaN NW arrays on transparent substrates. A detailed numerical analysis reveals that the transmission characteristics for large periods are determined by the waveguide character of the single NW, whereas for dense GaN NW arrays inter-wire coupling and diffraction effects originating from the periodic arrangement of the GaN NWs dominate the transmission. The numerically simulated results are confirmed by experimental transmission measurements. We also investigate the influence of a dielectric NW shell and of the surrounding medium on the transmission characteristics of a GaN NW array.

Optimizing the beam pattern of a forward-viewing ring-annular ultrasound array for intravascular imaging.

PubMed

Wang, Yao; Stephens, Douglas N; O'Donnell, Matthew

2002-12-01

Intravascular ultrasound (IVUS) imaging systems using circumferential arrays mounted on cardiac catheter tips fire beams orthogonal to the principal axis of the catheter. The system produces high resolution cross-sectional images but must be guided by conventional angioscopy. A real-time forward-viewing array, integrated into the same catheter, could greatly reduce radiation exposure by decreasing angiographic guidance. Unfortunately, the mounting requirement of a catheter guide wire prohibits a full-disk imaging aperture. Given only an annulus of array elements, prior theoretical investigations have only considered a circular ring of point transceivers and focusing strategies using all elements in the highly dense array, both impractical assumptions. In this paper, we consider a practical array geometry and signal processing architecture for a forward-viewing IVUS system. Our specific design uses a total of 210 transceiver firings with synthetic reconstruction for a given 3-D image frame. Simulation results demonstrate this design can achieve side-lobes under -40 dB for on-axis situations and under -30 dB for steering to the edge of a 80 degrees cone.

Using a coherent hydrophone array for observing sperm whale range, classification, and shallow-water dive profiles.

PubMed

Tran, Duong D; Huang, Wei; Bohn, Alexander C; Wang, Delin; Gong, Zheng; Makris, Nicholas C; Ratilal, Purnima

2014-06-01

Sperm whales in the New England continental shelf and slope were passively localized, in both range and bearing, and classified using a single low-frequency (<2500 Hz), densely sampled, towed horizontal coherent hydrophone array system. Whale bearings were estimated using time-domain beamforming that provided high coherent array gain in sperm whale click signal-to-noise ratio. Whale ranges from the receiver array center were estimated using the moving array triangulation technique from a sequence of whale bearing measurements. Multiple concurrently vocalizing sperm whales, in the far-field of the horizontal receiver array, were distinguished and classified based on their horizontal spatial locations and the inter-pulse intervals of their vocalized click signals. The dive profile was estimated for a sperm whale in the shallow waters of the Gulf of Maine with 160 m water-column depth located close to the array's near-field where depth estimation was feasible by employing time difference of arrival of the direct and multiply reflected click signals received on the horizontal array. By accounting for transmission loss modeled using an ocean waveguide-acoustic propagation model, the sperm whale detection range was found to exceed 60 km in low to moderate sea state conditions after coherent array processing.

A spatially resolved pyrometer for measuring the blackbody temperature of a warm dense plasma

DOE PAGES

Coleman, Joshua Eugene

2016-12-30

A pyrometer has been developed to spatially resolve the blackbody temperature of a radiatively cooling warm dense plasma. The pyrometer is composed of a lens coupled fiber array, Czerny-Turner visible spectrometer, and an intensified gated CCD for the detector. The radiatively cooling warm dense plasma is generated by a ~100-ns-long intense relativistic electron bunch with an energy of 19.1 MeV and a current of 0.2 kA interacting with 100-μm-thick low-Z foils. The continuum spectrum is measured over 250 nm with a low groove density grating. These plasmas emit visible light or blackbody radiation on relatively long time scales (~0.1 tomore » 100 μs). Finally, we presented the diagnostic layout, calibration, and proof-of-principle measurement of a radiatively cooling aluminum plasma, which includes a spatially resolved temperature gradient and the ability to temporally resolve it also.« less

Dense Vertically Aligned Copper Nanowire Composites as High Performance Thermal Interface Materials.

PubMed

Barako, Michael T; Isaacson, Scott G; Lian, Feifei; Pop, Eric; Dauskardt, Reinhold H; Goodson, Kenneth E; Tice, Jesse

2017-12-06

Thermal interface materials (TIMs) are essential for managing heat in modern electronics, and nanocomposite TIMs can offer critical improvements. Here, we demonstrate thermally conductive, mechanically compliant TIMs based on dense, vertically aligned copper nanowires (CuNWs) embedded into polymer matrices. We evaluate the thermal and mechanical characteristics of 20-25% dense CuNW arrays with and without polydimethylsiloxane infiltration. The thermal resistance achieved is below 5 mm 2 K W -1 , over an order of magnitude lower than commercial heat sink compounds. Nanoindentation reveals that the nonlinear deformation mechanics of this TIM are influenced by both the CuNW morphology and the polymer matrix. We also implement a flip-chip bonding protocol to directly attach CuNW composites to copper surfaces, as required in many thermal architectures. Thus, we demonstrate a rational design strategy for nanocomposite TIMs that simultaneously retain the high thermal conductivity of aligned CuNWs and the mechanical compliance of a polymer.

HCN and HCO(+) images of the photodissociation region in the Orion Bar

NASA Technical Reports Server (NTRS)

Youngowl, Rolaine C.; Meixner, Margaret; Tielens, Alexander G. G. M.; Tauber, Jan A.

1995-01-01

We present preliminary millimeter-wavelength images of the photodissociation region (PDR) in the Orion Bar, observed with the Berkeley- Illinois-Maryland array (BIMA). These new BIMA observations have attained 5 arc sec resolution in the J=l-O emission lines of HCO+ (formyl ion) and HCN (hydrogen cyanide). The results are compared with previous observations of the J=1-0 transition lines of (13)CO. We find that the HCO+ and HCN have different spatial distributions. HCN appears to lie primarily inside dense clumps of gas, which are defined by areas of intense (13)CO emission. However, the HCO+ emission appears to be only loosely associated with the surfaces of the gas clumps. We suggest that HCO+ abundance is enhanced by the presence of vibrationally excited H2 on the surfaces of dense clumps, and that the HCN abundance is attenuated by photo destruction outside the cores of dense clumps of gas.

Production of dense plasmas in a hypocycloidal pinch apparatus

NASA Technical Reports Server (NTRS)

Lee, J. H.; Mcfarland, D. R.; Hohl, F.

1977-01-01

A high-power pinch apparatus consisting of disk electrodes was developed, and diagnostic measurements to study its mechanism of dense plasma production have been made. The collapse fronts of the current sheets are well organized, and dense plasma foci are produced on the axis with radial stability in excess of 5 microsec. A plasma density greater than 10 to the 18th power per cu cm is determined with Stark broadening and CO2 laser absorption. Essentially complete absorption of a high-energy CO2 laser beam has been observed. A plasma temperature of approximately 1 keV is measured with differential transmission of soft X-rays through thin foils. The advantages of this apparatus over the coaxial plasma focus are improvements in (1) plasma volume, (2) stability, (3) containment time, (4) access to additional heating by laser or electron beams, and (5) the possibility of scaling up to a multiple array for high-power operation.

Dense plasma focus production in a hypocycloidal pinch

NASA Technical Reports Server (NTRS)

Lee, J. H.; Mcfarland, D. R.; Hohl, F.

1975-01-01

A type of high-power pinch apparatus consisting of disk electrodes was developed, and diagnostic measurements to study its mechanism of dense plasma production were made. The collapse fronts of the current sheets are well organized, and dense plasma focuses are produced on the axis with radial stability in excess of 5 microns. A plasma density greater than 10 to the 18th power/cubic cm was determined with Stark broadening and CO2 laser absorption. A plasma temperature of approximately 1 keV was measured with differential transmission of soft X-rays through thin foils. Essentially complete absorption of a high-energy CO2 laser beam was observed. The advantages of this apparatus over the coaxial plasma focus are in (1) the plasma volume, (2) the stability, (3) the containment time, (4) the easy access to additional heating by laser or electron beams, and (5) the possibility of scaling up to a multiple array for high-power operation.

Detecting Micro-seismicity and Long-duration Tremor-like Events from the Oklahoma Wavefield Experiment

NASA Astrophysics Data System (ADS)

Li, C.; Li, Z.; Peng, Z.; Zhang, C.; Nakata, N.

2017-12-01

Oklahoma has experienced abrupt increase of induced seismicity in the last decade. An important way to fully understand seismic activities in Oklahoma is to obtain more complete earthquake catalogs and detect different types of seismic events. The IRIS Community Wavefield Demonstration Experiment was deployed near Enid, Oklahoma in Summer of 2016. The dataset from this ultra-dense array provides an excellent opportunity for detecting microseismicity in that region with wavefield approaches. Here we examine continuous waveforms recorded by 3 seismic lines using local coherence for ultra-dense arrays (Li et al., 2017), which is a measure of cross-correlation of waveform at each station with its nearby stations. So far we have detected more than 5,000 events from 06/22/2016 to 07/20/2016, and majority of them are not listed on the regional catalog of Oklahoma or global catalogs, indicating that they are local events. We also identify 15-20 long-period long-duration events, some of them lasting for more than 500 s. Such events have been found at major plate-boundary faults (also known as deep tectonic tremor), as well as during hydraulic fracturing, slow-moving landslides and glaciers. Our next step is to locate these possible tremor-like events with their relative arrival times across the array and compare their occurrence times with solid-earth tides and injection histories to better understand their driving mechanisms.

Multicolor, time-gated, soft x-ray pinhole imaging of wire array and gas puff Z pinches on the Z and Saturn pulsed power generators.

PubMed

Jones, B; Coverdale, C A; Nielsen, D S; Jones, M C; Deeney, C; Serrano, J D; Nielsen-Weber, L B; Meyer, C J; Apruzese, J P; Clark, R W; Coleman, P L

2008-10-01

A multicolor, time-gated, soft x-ray pinhole imaging instrument is fielded as part of the core diagnostic set on the 25 MA Z machine [M. E. Savage et al., in Proceedings of the Pulsed Power Plasma Sciences Conference (IEEE, New York, 2007), p. 979] for studying intense wire array and gas puff Z-pinch soft x-ray sources. Pinhole images are reflected from a planar multilayer mirror, passing 277 eV photons with <10 eV bandwidth. An adjacent pinhole camera uses filtration alone to view 1-10 keV photons simultaneously. Overlaying these data provides composite images that contain both spectral as well as spatial information, allowing for the study of radiation production in dense Z-pinch plasmas. Cu wire arrays at 20 MA on Z show the implosion of a colder cloud of material onto a hot dense core where K-shell photons are excited. A 528 eV imaging configuration has been developed on the 8 MA Saturn generator [R. B. Spielman et al., and A. I. P. Conf, Proc. 195, 3 (1989)] for imaging a bright Li-like Ar L-shell line. Ar gas puff Z pinches show an intense K-shell emission from a zippering stagnation front with L-shell emission dominating as the plasma cools.

In Vivo Validation of Custom-Designed Silicon-Based Microelectrode Arrays for Long-Term Neural Recording and Stimulation

PubMed Central

Manoonkitiwongsa, Panya S.; Wang, Cindy X.; McCreery, Douglas B.

2012-01-01

We developed and validated silicon-based neural probes for neural stimulating and recording in long-term implantation in the brain. The probes combine the deep reactive ion etching process and mechanical shaping of their tip region, yielding a mechanically sturdy shank with a sharpened tip to reduce insertion force into the brain and spinal cord, particularly, with multiple shanks in the same array. The arrays’ insertion forces have been quantified in vitro. Five consecutive chronically-implanted devices were fully functional from 3 to 18 months. The microelectrode sites were electroplated with iridium oxide, and the charge injection capacity measurements were performed both in vitro and after implantation in the adult feline brain. The functionality of the chronic array was validated by stimulating in the cochlear nucleus and recording the evoked neuronal activity in the central nucleus of the inferior colliculus. The arrays’ recording quality has also been quantified in vivo with neuronal spike activity recorded up to 566 days after implantation. Histopathology evaluation of neurons and astrocytes using immunohistochemical stains indicated minimal alterations of tissue architecture after chronic implantation. PMID:22020666

Fault zone property near Xinfengjiang Reservoir using dense, across-fault seismic array

NASA Astrophysics Data System (ADS)

Lee, M. H. B.; Yang, H.; Sun, X.

2017-12-01

Properties of fault zones are important to the understanding of earthquake process. Around the fault zone is a damaged zone which is characterised by a lower seismic velocity. This is detectable as a low velocity zone and measure some physical property of the fault zone, which is otherwise difficult sample directly. A dense, across-fault array of short period seismometer is deployed on an inactive fault near Xinfengjiang Reservoir. Local events were manually picked. By computing the synthetic arrival time, we were able to constrain the parameters of the fault zone Preliminary result shows that the fault zone is around 350 m wide with a P and S velocity increase of around 10%. The fault is geologically inferred, and this result suggested that it may be a geological layer. The other possibility is that the higher velocity is caused by a combination of fault zone healing and fluid intrusion. Whilst the result was not able to tell us the nature of the fault, it demonstrated that this method is able to derive properties from a fault zone.

Optical response of nanostructured metal/dielectric composites and multilayers

NASA Astrophysics Data System (ADS)

Smith, Geoffrey B.; Maaroof, Abbas I.; Allan, Rodney S.; Schelm, Stefan; Anstis, Geoffrey R.; Cortie, Michael B.

2004-08-01

The homogeneous optical response in conducting nanostructured layers, and in insulating layers containing dense arrays of self assembled conducting nanoparticles separated by organic linkers, is examined experimentally through their effective complex indices (n*, k*). Classical effective medium models, modified to account for the 3-phase nanostructure, are shown to explain (n*, k*) in dense particulate systems but not inhomogeneous layers with macroscopic conductance for which a different approach to homogenisation is discussed. (n*, k*) data on thin granular metal films, thin mesoporous gold, and on thin metal layers containing ordered arrays of voids, is linked to properties of the surface plasmon states which span the nanostructured film. Coupling between evanescent waves at either surface counterbalanced by electron scattering losses must be considered. Virtual bound states for resonant photons result, with the associated transit delay leading to a large rise in n* in many nanostructures. Overcoating n-Ag with alumina is shown to alter (n*, k*) through its impact on the SP coupling. In contrast to classical optical homogenisation, effective indices depend on film thickness. Supporting high resolution SEM images are presented.

Resting State Dense Array Gamma Oscillatory Activity as a Response Marker for Cerebellar-Repetitive Transcranial Magnetic Stimulation (rTMS) in Schizophrenia.

PubMed

Tikka, Sai Krishna; Garg, Shobit; Sinha, Vinod Kumar; Nizamie, S Haque; Goyal, Nishant

2015-12-01

As cerebellum and its abnormalities have been implicated in the pathophysiology of schizophrenia, repetitive transcranial magnetic stimulation (rTMS) of this alternate site has been suggested as a novel target for treating patients with this disorder. As resting state gamma activity measures functional brain connectivity, it could be used as a specific treatment marker. To investigate the effect of cerebellar-rTMS on resting state gamma activity, while studying its efficacy in recent onset schizophrenia patients. This rater-blinded prospective study was completed by 11 schizophrenia patients. They received 10 sessions of high-frequency (theta patterned) rTMS to midline cerebellum over 2 weeks. Resting state EEG was recorded using high (192-channel) resolution EEG at baseline and post rTMS. Gamma spectral power was calculated using fast Fourier transformation, Hanning window averaged over 8 scalp segments corresponding 8 lobes. Clinical improvement rated on the Positive and Negative Syndrome Scale and depressive symptoms assessed using the Calgary Depression Scale for Schizophrenia were other outcome variables. Nonparametric statistics were used. Over the treatment course, significant reduction was seen on negative syndrome and depression scores. Gamma spectral power in left frontal and temporal segments reduced significantly. Spearman correlation analysis showed that percentage reduction in psychopathology scores had significant positive correlation with percentage reduction in gamma spectral power. Cerebellar-rTMS might be an effective adjunct to treat intricate and lingering negative and affective symptoms. Resting state gamma spectral power in frontal and temporal regions might be used as a biomarker for treatment response.

RESIF Seismology Datacentre : Recently Released Data and New Services. Computing with Dense Seisimic Networks Data.

NASA Astrophysics Data System (ADS)

Volcke, P.; Pequegnat, C.; Grunberg, M.; Lecointre, A.; Bzeznik, B.; Wolyniec, D.; Engels, F.; Maron, C.; Cheze, J.; Pardo, C.; Saurel, J. M.; André, F.

2015-12-01

RESIF is a nationwide french project aimed at building a high quality observation system to observe and understand the inner earth. RESIF deals with permanent seismic networks data as well as mobile networks data, including dense/semi-dense arrays. RESIF project is distributed among different nodes providing qualified data to the main datacentre in Université Grenoble Alpes, France. Data control and qualification is performed by each individual nodes : the poster will provide some insights on RESIF broadband seismic component data quality control. We will then present data that has been recently made publicly available. Data is distributed through worldwide FDSN and european EIDA standards protocols. A new web portal is now opened to explore and download seismic data and metadata. The RESIF datacentre is also now connected to Grenoble University High Performance Computing (HPC) facility : a typical use-case will be presented using iRODS technologies. The use of dense observation networks is increasing, bringing challenges in data growth and handling : we will present an example where HDF5 data format was used as an alternative to usual seismology data formats.

High-Resolution Strain Analysis of the Human Heart with Fast-DENSE

NASA Astrophysics Data System (ADS)

Aletras, Anthony H.; Balaban, Robert S.; Wen, Han

1999-09-01

Single breath-hold displacement data from the human heart were acquired with fast-DENSE (fast displacement encoding with stimulated echoes) during systolic contraction at 2.5 × 2.5 mm in-plane resolution. Encoding strengths of 0.86-1.60 mm/π were utilized in order to extend the dynamic range of the phase measurements and minimize effects of physiologic and instrument noise. The noise level in strain measurements for both contraction and dilation corresponded to a strain value of 2.8%. In the human heart, strain analysis has sufficient resolution to reveal transmural variation across the left ventricular wall. Data processing required minimal user intervention and provided a rapid quantitative feedback. The intrinsic temporal integration of fast-DENSE achieves high accuracy at the expense of temporal resolution.

Carbon Nanofiber Electrode Array for Neurochemical Monitoring

NASA Technical Reports Server (NTRS)

Koehne, Jessica E.

2017-01-01

A sensor platform based on vertically aligned carbon nanofibers (CNFs) has been developed. Their inherent nanometer scale, high conductivity, wide potential window, good biocompatibility and well-defined surface chemistry make them ideal candidates as biosensor electrodes. Here, we report using vertically aligned CNF as neurotransmitter recording electrodes for application in a smart deep brain stimulation (DBS) device. Our approach combines a multiplexed CNF electrode chip, developed at NASA Ames Research Center, with the Wireless Instantaneous Neurotransmitter Concentration Sensor (WINCS) system, developed at the Mayo Clinic. Preliminary results indicate that the CNF nanoelectrode arrays are easily integrated with WINCS for neurotransmitter detection in a multiplexed array format. In the future, combining CNF based stimulating and recording electrodes with WINCS may lay the foundation for an implantable smart therapeutic system that utilizes neurochemical feedback control while likely resulting in increased DBS application in various neuropsychiatric disorders. In total, our goal is to take advantage of the nanostructure of CNF arrays for biosensing studies requiring ultrahigh sensitivity, high-degree of miniaturization, and selective biofunctionalization.

A Simple and Accurate Model to Predict Responses to Multi-electrode Stimulation in the Retina

PubMed Central

Maturana, Matias I.; Apollo, Nicholas V.; Hadjinicolaou, Alex E.; Garrett, David J.; Cloherty, Shaun L.; Kameneva, Tatiana; Grayden, David B.; Ibbotson, Michael R.; Meffin, Hamish

2016-01-01

Implantable electrode arrays are widely used in therapeutic stimulation of the nervous system (e.g. cochlear, retinal, and cortical implants). Currently, most neural prostheses use serial stimulation (i.e. one electrode at a time) despite this severely limiting the repertoire of stimuli that can be applied. Methods to reliably predict the outcome of multi-electrode stimulation have not been available. Here, we demonstrate that a linear-nonlinear model accurately predicts neural responses to arbitrary patterns of stimulation using in vitro recordings from single retinal ganglion cells (RGCs) stimulated with a subretinal multi-electrode array. In the model, the stimulus is projected onto a low-dimensional subspace and then undergoes a nonlinear transformation to produce an estimate of spiking probability. The low-dimensional subspace is estimated using principal components analysis, which gives the neuron’s electrical receptive field (ERF), i.e. the electrodes to which the neuron is most sensitive. Our model suggests that stimulation proportional to the ERF yields a higher efficacy given a fixed amount of power when compared to equal amplitude stimulation on up to three electrodes. We find that the model captures the responses of all the cells recorded in the study, suggesting that it will generalize to most cell types in the retina. The model is computationally efficient to evaluate and, therefore, appropriate for future real-time applications including stimulation strategies that make use of recorded neural activity to improve the stimulation strategy. PMID:27035143

C%2B%2B tensor toolbox user manual.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Plantenga, Todd D.; Kolda, Tamara Gibson

2012-04-01

The C++ Tensor Toolbox is a software package for computing tensor decompositions. It is based on the Matlab Tensor Toolbox, and is particularly optimized for sparse data sets. This user manual briefly overviews tensor decomposition mathematics, software capabilities, and installation of the package. Tensors (also known as multidimensional arrays or N-way arrays) are used in a variety of applications ranging from chemometrics to network analysis. The Tensor Toolbox provides classes for manipulating dense, sparse, and structured tensors in C++. The Toolbox compiles into libraries and is intended for use with custom applications written by users.

Highly organised and dense vertical silicon nanowire arrays grown in porous alumina template on <100> silicon wafers

PubMed Central

2013-01-01

In this work, nanoimprint lithography combined with standard anodization etching is used to make perfectly organised triangular arrays of vertical cylindrical alumina nanopores onto standard <100>−oriented silicon wafers. Both the pore diameter and the period of alumina porous array are well controlled and can be tuned: the periods vary from 80 to 460 nm, and the diameters vary from 15 nm to any required diameter. These porous thin layers are then successfully used as templates for the guided epitaxial growth of organised mono-crystalline silicon nanowire arrays in a chemical vapour deposition chamber. We report the densities of silicon nanowires up to 9 × 109 cm−2 organised in highly regular arrays with excellent diameter distribution. All process steps are demonstrated on surfaces up to 2 × 2 cm2. Specific emphasis was made to select techniques compatible with microelectronic fabrication standards, adaptable to large surface samples and with a reasonable cost. Achievements made in the quality of the porous alumina array, therefore on the silicon nanowire array, widen the number of potential applications for this technology, such as optical detectors or biological sensors. PMID:23773702

A Micro-Electrode Array device coupled to a laser-based system for the local stimulation of neurons by optical release of glutamate.

PubMed

Ghezzi, Diego; Menegon, Andrea; Pedrocchi, Alessandra; Valtorta, Flavia; Ferrigno, Giancarlo

2008-10-30

Optical stimulation is a promising approach to investigate the local dynamic responses of cultured neurons. In particular, flash photolysis of caged compounds offers the advantage of allowing the rapid change of concentration of either extracellular or intracellular molecules, such as neurotransmitters or second messengers, for the stimulation or modulation of neuronal activity. We describe here the use of an ultra-violet (UV) laser diode coupled to an optical fibre for the local activation of caged compounds combined with a Micro-Electrode Array (MEA) device. Local uncaging was achieved by UV irradiation through the optical fibre previously positioned by using a red laser diode. The size of the stimulation was determined using caged fluorescein, whereas its efficacy was tested by studying the effect of uncaging the neurotransmitter glutamate. Uncaged glutamate evoked neuronal responses that were recorded using either fluorescence measurements or electrophysiological recordings with MEAs, thus showing the ability of our system to induce local neuronal excitation. This method allows overcoming the limitations of the MEA system related to unfocused electrical stimulation and induction of electrical artefacts. In addition, the coupling of a UV laser diode to an optical fibre allows a precise local stimulation and a quick change of the stimulation point.

On the Viability of Using Autonomous Three-Component Nodal Geophones to Calculate Teleseismic Ps Receiver Functions with an Application to the Old Faithful Hydrothermal System and the Cascadia Subduction Zone

NASA Astrophysics Data System (ADS)

Ward, K. M.; Lin, F. C.

2017-12-01

Recent advances in seismic data-acquisition technology paired with an increasing interest from the academic passive source seismological community have opened up new scientific targets and imaging possibilities, often referred to as Large-N experiments (large number of instruments). The success of these and other deployments has motivated individual researchers, as well as the larger seismological community, to invest in the next generation of nodal geophones. Although the new instruments have battery life and bandwidth limitations compared to broadband instruments, the relatively low deployment and procurement cost of these new nodal geophones provides an additional novel tool for researchers. Here, we explore the viability of using autonomous three-component nodal geophones to calculate teleseismic Ps receiver functions by comparison of co-located broadband stations and highlight some potential advantages with a dense nodal array deployed around the Upper Geyser basin in Yellowstone National Park. Two key findings from this example include (1) very dense nodal arrays can be used to image small-scale features in the shallow crust that typical broadband station spacing would alias, and (2) nodal arrays with a larger footprint could be used to image deeper features with greater or equal detail as typical broadband deployments but at a reduced deployment cost. The success of the previous example has motivated a larger 2-D line across the Cascadia subduction zone. In the summer of 2017, we deployed 174 nodal geophones with an average site spacing of 750 m. Synthetic tests with dense station spacing ( 1 km) reveal subtler features of the system that is consistent with our preliminary receiver function results from our Cascadia deployment. With the increasing availability of nodal geophones to individual researchers and the successful demonstration that nodal geophones are a viable instrument for receiver function studies, numerous scientific targets can be investigated at reduced costs or in expanded detail.

Reading Visual Braille with a Retinal Prosthesis

PubMed Central

Lauritzen, Thomas Z.; Harris, Jordan; Mohand-Said, Saddek; Sahel, Jose A.; Dorn, Jessy D.; McClure, Kelly; Greenberg, Robert J.

2012-01-01

Retinal prostheses, which restore partial vision to patients blinded by outer retinal degeneration, are currently in clinical trial. The Argus II retinal prosthesis system was recently awarded CE approval for commercial use in Europe. While retinal prosthesis users have achieved remarkable visual improvement to the point of reading letters and short sentences, the reading process is still fairly cumbersome. This study investigates the possibility of using an epiretinal prosthesis to stimulate visual braille as a sensory substitution for reading written letters and words. The Argus II retinal prosthesis system, used in this study, includes a 10 × 6 electrode array implanted epiretinally, a tiny video camera mounted on a pair of glasses, and a wearable computer that processes the video and determines the stimulation current of each electrode in real time. In the braille reading system, individual letters are created by a subset of dots from a 3 by 2 array of six dots. For the visual braille experiment, a grid of six electrodes was chosen out of the 10 × 6 Argus II array. Groups of these electrodes were then directly stimulated (bypassing the camera) to create visual percepts of individual braille letters. Experiments were performed in a single subject. Single letters were stimulated in an alternative forced choice (AFC) paradigm, and short 2–4-letter words were stimulated (one letter at a time) in an open-choice reading paradigm. The subject correctly identified 89% of single letters, 80% of 2-letter, 60% of 3-letter, and 70% of 4-letter words. This work suggests that text can successfully be stimulated and read as visual braille in retinal prosthesis patients. PMID:23189036

Reading visual braille with a retinal prosthesis.

PubMed

Lauritzen, Thomas Z; Harris, Jordan; Mohand-Said, Saddek; Sahel, Jose A; Dorn, Jessy D; McClure, Kelly; Greenberg, Robert J

2012-01-01

Retinal prostheses, which restore partial vision to patients blinded by outer retinal degeneration, are currently in clinical trial. The Argus II retinal prosthesis system was recently awarded CE approval for commercial use in Europe. While retinal prosthesis users have achieved remarkable visual improvement to the point of reading letters and short sentences, the reading process is still fairly cumbersome. This study investigates the possibility of using an epiretinal prosthesis to stimulate visual braille as a sensory substitution for reading written letters and words. The Argus II retinal prosthesis system, used in this study, includes a 10 × 6 electrode array implanted epiretinally, a tiny video camera mounted on a pair of glasses, and a wearable computer that processes the video and determines the stimulation current of each electrode in real time. In the braille reading system, individual letters are created by a subset of dots from a 3 by 2 array of six dots. For the visual braille experiment, a grid of six electrodes was chosen out of the 10 × 6 Argus II array. Groups of these electrodes were then directly stimulated (bypassing the camera) to create visual percepts of individual braille letters. Experiments were performed in a single subject. Single letters were stimulated in an alternative forced choice (AFC) paradigm, and short 2-4-letter words were stimulated (one letter at a time) in an open-choice reading paradigm. The subject correctly identified 89% of single letters, 80% of 2-letter, 60% of 3-letter, and 70% of 4-letter words. This work suggests that text can successfully be stimulated and read as visual braille in retinal prosthesis patients.

Studies Of Infrasonic Propagation Using Dense Seismic Networks

NASA Astrophysics Data System (ADS)

Hedlin, M. A.; deGroot-Hedlin, C. D.; Drob, D. P.

2011-12-01

Although there are approximately 100 infrasonic arrays worldwide, more than ever before, the station density is still insufficient to provide validation for detailed propagation modeling. Relatively large infrasonic signals can be observed on seismic channels due to coupling at the Earth's surface. Recent research, using data from the 70-km spaced 400-station USArray and other seismic network deployments, has shown the value of dense seismic network data for filling in the gaps between infrasonic arrays. The dense sampling of the infrasonic wavefield has allowed us to observe complete travel-time branches of infrasound and address important research problems in infrasonic propagation. We present our analysis of infrasound created by a series of rocket motor detonations that occurred at the UTTR facility in Utah in 2007. These data were well recorded by the USArray seismometers. We use the precisely located blasts to assess the utility of G2S mesoscale models and methods to synthesize infrasonic propagation. We model the travel times of the branches using a ray-based approach and the complete wavefield using a FDTD algorithm. Although results from both rays and FDTD approaches predict the travel times to within several seconds, only about 40% of signals are predicted using rays largely due to penetration of sound into shadow zones. FDTD predicts some sound penetration into the shadow zone, but the observed shadow zones, as defined by the seismic data, have considerably narrower spatial extent than either method predicts, perhaps due to un-modeled small-scale structure in the atmosphere.

Reconstruction of Microraptor and the evolution of iridescent plumage.

PubMed

Li, Quanguo; Gao, Ke-Qin; Meng, Qingjin; Clarke, Julia A; Shawkey, Matthew D; D'Alba, Liliana; Pei, Rui; Ellison, Mick; Norell, Mark A; Vinther, Jakob

2012-03-09

Iridescent feather colors involved in displays of many extant birds are produced by nanoscale arrays of melanin-containing organelles (melanosomes). Data relevant to the evolution of these colors and the properties of melanosomes involved in their generation have been limited. A data set sampling variables of extant avian melanosomes reveals that those forming most iridescent arrays are distinctly narrow. Quantitative comparison of these data with melanosome imprints densely sampled from a previously unknown specimen of the Early Cretaceous feathered Microraptor predicts that its plumage was predominantly iridescent. The capacity for simple iridescent arrays is thus minimally inferred in paravian dinosaurs. This finding and estimation of Microraptor feathering consistent with an ornamental function for the tail suggest a centrality for signaling in early evolution of plumage and feather color.

Miniature micromachined quadrupole mass spectrometer array and method of making the same

NASA Technical Reports Server (NTRS)

Fuerstenau, Stephen D. (Inventor); Yee, Karl Y. (Inventor); Chutjian, Ara (Inventor); Orient, Otto J. (Inventor); Rice, John T. (Inventor)

2002-01-01

The present invention provides a quadrupole mass spectrometer and an ion filter, or pole array, for use in the quadrupole mass spectrometer. The ion filter includes a thin patterned layer including a two-dimensional array of poles forming one or more quadrupoles. The patterned layer design permits the use of very short poles and with a very dense spacing of the poles, so that the ion filter may be made very small. Also provided is a method for making the ion filter and the quadrupole mass spectrometer. The method involves forming the patterned layer of the ion filter in such a way that as the poles of the patterned layer are formed, they have the relative positioning and alignment for use in a final quadrupole mass spectrometer device.

Miniature micromachined quadrupole mass spectrometer array and method of making the same

NASA Technical Reports Server (NTRS)

Chutjian, Ara (Inventor); Rice, John T. (Inventor); Fuerstenau, Stephen D. (Inventor); Orient, Otto J. (Inventor); Yee, Karl Y. (Inventor)

2000-01-01

The present invention provides a quadrupole mass spectrometer and an ion filter, or pole array, for use in the quadrupole mass spectrometer. The ion filter includes a thin patterned layer including a two-dimensional array of poles forming one or more quadrupoles. The patterned layer design permits the use of very short poles and with a very dense spacing of the poles, so that the ion filter may be made very small. Also provided is a method for making the ion filter and the quadrupole mass spectrometer. The method involves forming the patterned layer of the ion filter in such a way that as the poles of the patterned layer are formed, they have the relative positioning and alignment for use in a final quadrupole mass spectrometer device.

Miniature micromachined quadrupole mass spectrometer array and method of making the same

NASA Technical Reports Server (NTRS)

Yee, Karl Y. (Inventor); Fuerstenau, Stephen D. (Inventor); Orient, Otto J. (Inventor); Rice, John T. (Inventor); Chutjian, Ara (Inventor)

2001-01-01

The present invention provides a quadrupole mass spectrometer and an ion filter, or pole array, for use in the quadrupole mass spectrometer. The ion filter includes a thin patterned layer including a two-dimensional array of poles forming one or more quadrupoles. The patterned layer design permits the use of very short poles and with a very dense spacing of the poles, so that the ion filter may be made very small. Also provided is a method for making the ion filter and the quadrupole mass spectrometer. The method involves forming the patterned layer of the ion filter in such a way that as the poles of the patterned layer are formed, they have the relative positioning and alignment for use in a final quadrupole mass spectrometer device.

High yield of self-catalyzed GaAs nanowire arrays grown on silicon via gallium droplet positioning.

PubMed

Plissard, S; Larrieu, G; Wallart, X; Caroff, P

2011-07-08

We report and detail a method to achieve growth of vertical self-catalyzed GaAs nanowires directly on Si(111) with a near-perfect vertical yield, using electron-beam-defined arrays of holes in a dielectric layer and molecular beam epitaxy. In our conditions, GaAs nanowires are grown along a vapor-liquid-solid mechanism, using in situ self-forming Ga droplets. The focus of this paper is to understand the role of the substrate preparation and of the pre-growth conditioning. Without changing temperature or the V/III ratio, the yield of vertical nanowires is increased incrementally up to 95%. The possibility to achieve very dense arrays, with center-to-center inter-wire distances less than 100 nm, is demonstrated.

Pixel detectors for use in retina neurophysiology studies

NASA Astrophysics Data System (ADS)

Cunningham, W.; Mathieson, K.; Horn, M.; Melone, J.; McEwan, F. A.; Blue, A.; O'Shea, V.; Smith, K. M.; Litke, A.; Chichilnisky, E. J.; Rahman, M.

2003-08-01

One area of major inter-disciplinary co-operation is between the particle physics and bio-medical communities. The type of large detector arrays and fast electronics developed in laboratories like CERN are becoming used for a wide range of medical and biological experiments. In the present work fabrication technology developed for producing semiconductor radiation detectors has been applied to produce arrays which have been used in neuro-physiological experiments on retinal tissue. We have exploited UVIII, a low molecular weight resist, that has permitted large area electron beam lithography. This allows the resolution to go below that of conventional photolithography and hence the production of densely packed ˜500 electrode arrays with feature sizes down to below 2 μm. The neural signals from significant areas of the retina may thus be captured.

Optimal configuration of respiratory navigator gating for the quantification of left ventricular strain using spiral cine displacement encoding with stimulated echoes (DENSE) MRI.

PubMed

Hamlet, Sean M; Haggerty, Christopher M; Suever, Jonathan D; Wehner, Gregory J; Andres, Kristin N; Powell, David K; Zhong, Xiaodong; Fornwalt, Brandon K

2017-03-01

To determine the optimal respiratory navigator gating configuration for the quantification of left ventricular strain using spiral cine displacement encoding with stimulated echoes (DENSE) MRI. Two-dimensional spiral cine DENSE was performed on a 3 Tesla MRI using two single-navigator configurations (retrospective, prospective) and a combined "dual-navigator" configuration in 10 healthy adults and 20 healthy children. The adults also underwent breathhold DENSE as a reference standard for comparisons. Peak left ventricular strains, signal-to-noise ratio (SNR), and navigator efficiency were compared. Subjects also underwent dual-navigator gating with and without visual feedback to determine the effect on navigator efficiency. There were no differences in circumferential, radial, and longitudinal strains between navigator-gated and breathhold DENSE (P = 0.09-0.95) (as confidence intervals, retrospective: [-1.0%-1.1%], [-7.4%-2.0%], [-1.0%-1.2%]; prospective: [-0.6%-2.7%], [-2.8%-8.3%], [-0.3%-2.9%]; dual: [-1.6%-0.5%], [-8.3%-3.2%], [-0.8%-1.9%], respectively). The dual configuration maintained SNR compared with breathhold acquisitions (16 versus 18, P = 0.06). SNR for the prospective configuration was lower than for the dual navigator in adults (P = 0.004) and children (P < 0.001). Navigator efficiency was higher (P < 0.001) for both retrospective (54%) and prospective (56%) configurations compared with the dual configuration (35%). Visual feedback improved the dual configuration navigator efficiency to 55% (P < 0.001). When quantifying left ventricular strains using spiral cine DENSE MRI, a dual navigator configuration results in the highest SNR in adults and children. In adults, a retrospective configuration has good navigator efficiency without a substantial drop in SNR. Prospective gating should be avoided because it has the lowest SNR. Visual feedback represents an effective option to maintain navigator efficiency while using a dual navigator configuration. 2 J. Magn. Reson. Imaging 2017;45:786-794. © 2016 International Society for Magnetic Resonance in Medicine.

Optimal Configuration of Respiratory Navigator Gating for the Quantification of Left Ventricular Strain Using Spiral Cine Displacement Encoding with Stimulated Echoes (DENSE) MRI

PubMed Central

Hamlet, Sean M.; Haggerty, Christopher M.; Suever, Jonathan D.; Wehner, Gregory J.; Andres, Kristin N.; Powell, David K.; Fornwalt, Brandon K.

2016-01-01

Purpose To determine the optimal respiratory navigator gating configuration for the quantification of left ventricular strain using spiral cine displacement encoding with stimulated echoes (DENSE) MRI. Materials and Methods 2D spiral cine DENSE was performed on a 3T MRI using two single-navigator configurations (retrospective, prospective), and a combined “dual-navigator” configuration in 10 healthy adults and 20 healthy children. The adults also underwent breath-hold DENSE as a reference standard for comparisons. Peak left ventricular strains, signal-to-noise ratio (SNR) and navigator efficiency were compared. Subjects also underwent dual-navigator gating with and without visual feedback to determine the effect on navigator efficiency. Results There were no differences in circumferential, radial and longitudinal strains between navigator-gated and breath-hold DENSE (p=0.09–0.95) (as confidence intervals, retrospective: [−1.0%,1.1%],[−7.4%,2.0%],[−1.0%,1.2%]; prospective: [−0.6%,2.7%],[−2.8%,8.3%],[−0.3%,2.9%]; dual: [−1.6%,0.5%],[−8.3%,3.2%],[−0.8%,1.9%], respectively). The dual configuration maintained SNR compared to breath-hold acquisitions (16 vs. 18, p=0.06). SNR for the prospective configuration was lower than for the dual navigator in adults (p=0.004) and children (p<0.001). Navigator efficiency was higher (p<0.001) for both retrospective (54%) and prospective (56%) configurations compared to the dual configuration (35%). Visual feedback improved the dual configuration navigator efficiency to 55% (p<0.001). Conclusion When quantifying left ventricular strains using spiral cine DENSE MRI, a dual navigator configuration results in the highest SNR in adults and children. In adults, a retrospective configuration has good navigator efficiency without a substantial drop in SNR. Prospective gating should be avoided since it has the lowest SNR. Visual feedback represents an effective option to maintain navigator efficiency while using a dual navigator configuration. PMID:27458823

Fly's eye condenser based on chirped microlens arrays

NASA Astrophysics Data System (ADS)

Wippermann, Frank C.; Zeitner, Uwe-D.; Dannberg, Peter; Bräuer, Andreas; Sinzinger, Stefan

2007-09-01

Lens array arrangements are commonly used for the beam shaping of almost arbitrary input intensity distributions into a top-hat. The setup usually consists of a Fourier lens and two identical regular microlens arrays - often referred to as tandem lens array - where the second one is placed in the focal plane of the first microlenses. Due to the periodic structure of regular arrays the output intensity distribution is modulated by equidistant sharp intensity peaks which are disturbing the homogeneity. The equidistantly located intensity peaks can be suppressed when using a chirped and therefore non-periodic microlens array. A far field speckle pattern with more densely and irregularly located intensity peaks results leading to an improved homogeneity of the intensity distribution. In contrast to stochastic arrays, chirped arrays consist of individually shaped lenses defined by a parametric description of the cells optical function which can be derived completely from analytical functions. This gives the opportunity to build up tandem array setups enabling to achieve far field intensity distribution with an envelope of a top-hat. We propose a new concept for fly's eye condensers incorporating a chirped tandem microlens array for the generation of a top-hat far field intensity distribution with improved homogenization under coherent illumination. The setup is compliant to reflow of photoresist as fabrication technique since plane substrates accommodating the arrays are used. Considerations for the design of the chirped microlens arrays, design rules, wave optical simulations and measurements of the far field intensity distributions are presented.

A novel coil array for combined TMS/fMRI experiments at 3 T.

PubMed

Navarro de Lara, Lucia I; Windischberger, Christian; Kuehne, Andre; Woletz, Michael; Sieg, Jürgen; Bestmann, Sven; Weiskopf, Nikolaus; Strasser, Bernhard; Moser, Ewald; Laistler, Elmar

2015-11-01

To overcome current limitations in combined transcranial magnetic stimulation (TMS) and functional magnetic resonance imaging (fMRI) studies by employing a dedicated coil array design for 3 Tesla. The state-of-the-art setup for concurrent TMS/fMRI is to use a large birdcage head coil, with the TMS between the subject's head and the MR coil. This setup has drawbacks in sensitivity, positioning, and available imaging techniques. In this study, an ultraslim 7-channel receive-only coil array for 3 T, which can be placed between the subject's head and the TMS, is presented. Interactions between the devices are investigated and the performance of the new setup is evaluated in comparison to the state-of-the-art setup. MR sensitivity obtained at the depth of the TMS stimulation is increased by a factor of five. Parallel imaging with an acceleration factor of two is feasible with low g-factors. Possible interactions between TMS and the novel hardware were investigated and were found negligible. The novel coil array is safe, strongly improves signal-to-noise ratio in concurrent TMS/fMRI experiments, enables parallel imaging, and allows for flexible positioning of the TMS on the head while ensuring efficient TMS stimulation due to its ultraslim design. © 2014 The Authors. Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance in Medicine.

Application of Seismic Array Processing to Tsunami Early Warning

NASA Astrophysics Data System (ADS)

An, C.; Meng, L.

2015-12-01

Tsunami wave predictions of the current tsunami warning systems rely on accurate earthquake source inversions of wave height data. They are of limited effectiveness for the near-field areas since the tsunami waves arrive before data are collected. Recent seismic and tsunami disasters have revealed the need for early warning to protect near-source coastal populations. In this work we developed the basis for a tsunami warning system based on rapid earthquake source characterisation through regional seismic array back-projections. We explored rapid earthquake source imaging using onshore dense seismic arrays located at regional distances on the order of 1000 km, which provides faster source images than conventional teleseismic back-projections. We implement this method in a simulated real-time environment, and analysed the 2011 Tohoku earthquake rupture with two clusters of Hi-net stations in Kyushu and Northern Hokkaido, and the 2014 Iquique event with the Earthscope USArray Transportable Array. The results yield reasonable estimates of rupture area, which is approximated by an ellipse and leads to the construction of simple slip models based on empirical scaling of the rupture area, seismic moment and average slip. The slip model is then used as the input of the tsunami simulation package COMCOT to predict the tsunami waves. In the example of the Tohoku event, the earthquake source model can be acquired within 6 minutes from the start of rupture and the simulation of tsunami waves takes less than 2 min, which could facilitate a timely tsunami warning. The predicted arrival time and wave amplitude reasonably fit observations. Based on this method, we propose to develop an automatic warning mechanism that provides rapid near-field warning for areas of high tsunami risk. The initial focus will be Japan, Pacific Northwest and Alaska, where dense seismic networks with the capability of real-time data telemetry and open data accessibility, such as the Japanese HiNet (>800 instruments) and the Earthscope USArray Transportable Array (~400 instruments), are established.

Quality of Green's Functions Improved by Automatic Detection and Removal of Coherent Anthropogenic Noise

NASA Astrophysics Data System (ADS)

Williams, E. F.; Martin, E. R.; Biondi, B. C.; Lindsey, N.; Ajo Franklin, J. B.; Wagner, A. M.; Bjella, K.; Daley, T. M.; Dou, S.; Freifeld, B. M.; Robertson, M.; Ulrich, C.

2016-12-01

We analyze the impact of identifying and removing coherent anthropogenic noise on synthetic Green's functions extracted from ambient noise recorded on a dense linear distributed acoustic sensing (DAS) array. Low-cost, low-impact urban seismic surveys are possible with DAS, which uses dynamic strain sensing to record seismic waves incident to a buried fiber optic cable. However, interferometry and tomography of ambient noise data recorded in urban areas include coherent noise from near-field infrastructure such as cars and trains passing the array, in some cases causing artifacts in estimated Green's functions and potentially incorrect surface wave velocities. Based on our comparison of several methods, we propose an automated, real-time data processing workflow to detect and reduce the impact of these events on data from a dense array in an urban environment. We utilize a recursive STA/LTA (short-term average/long-term average) algorithm on each channel to identify sharp amplitude changes typically associated with an event arrival. In order to distinguish between optical noise and physical events, an event is cataloged only if STA/LTA is triggered on enough channels across the array in a short time window. For each event in the catalog, a conventional semblance analysis is performed across a straight segment of the array to determine whether the event has a coherent velocity signature. Events that demonstrate a semblance peak at low apparent velocities (5-50 m/s) are assumed to represent coherent transportation-related noise and are down-weighted in the time domain before cross-correlation. We show the impact of removing such noise on estimated Green's functions from ambient noise data recorded in Richmond, CA in December 2014. This method has been developed for use on a continuous time-lapse ambient noise survey collected with DAS near Fairbanks, AK, and an upcoming ambient noise survey on the Stanford University campus using DAS with a re-purposed telecommunications fiber optic cable.

Modular Total Syntheses of Hyperforin, Papuaforins A, B, and C via Gold(I)-Catalyzed Carbocyclization.

PubMed

Bellavance, Gabriel; Barriault, Louis

2018-05-15

The remarkable biological activities of polyprenylated polycyclic acylphloroglucinols (PPAPs) combined with their highly oxygenated and densely functionalized frameworks have stimulated the interest of synthetic organic chemists over the past decade. Herein, we report the concise total syntheses of four natural products PPAPs, of which some have antibacterial properties, notably hyperforin and papuaforin A. The salient features of this strategy are the short and gram-scalable synthesis of densely substituted PPAPs scaffolds via a Au(I)-catalyzed carbocyclization and the late-stage functionalization for a unified access to a wide variety of PPAPs.

Use of Microtremor Array Recordings for Mapping Subsurface Soil Structure, Singapore

NASA Astrophysics Data System (ADS)

Walling, M.

2012-12-01

Microtremor array recordings are carried out in Singapore, for different geology, to study the influence of each site in modeling the subsurface structure. The Spatial Autocorrelation (SPAC) method is utilized for the computation of the soil profiles. The array configuration of the recording consists of 7 seismometers, recording the vertical component of the ground motion, and the recording at each site is carried out for 30 minutes. The results from the analysis show that the soil structure modeled for the young alluvial of Kallang Formation (KF), in terms of shear wave velocity (Vs), gives a good correlation with borehole information, while for the older geological formation of Jurong Formation (JF) (sedimentary rock sequence) and Old Alluvial (OA) (dense alluvium formation), the correlation is not very clear due to the lack of impedance contrast. The older formation of Bukit Timah Granite (BTG) show contrasting results within the formation, with the northern BTG suggesting a low Vs upper layer of about 20m - 30m while the southern BTG reveals a dense formation. The discrepancy in the variation within BTG is confirmed from borehole data that reveals the northern BTG to have undergone intense weathering while the southern BTG have not undergone noticeable weathering. Few sites with bad recording quality could not resolve the soil structure. Microtremor array recording is good for mapping sites with soft soil formation and weathered rock formation but can be limited in the absence of subsurface velocity contrast and bad quality of microtremor records.; The correlation between the Vs30 estimated from SPAC method and borehole data for the four major geological formations of Singapore. The encircled sites are the sites with recording error.

High-resolution shallow structure revealed with ambient noise tomography on a dense array

NASA Astrophysics Data System (ADS)

Zeng, X.; Thurber, C. H.; Luo, Y.; Matzel, E.; Team, P.

2016-12-01

A dense seismic array was deployed by the PoroTomo research team at Brady Hot Springs, Nevada in March 2016. The array consisted of 238 short-period three-component geophones (5-Hz corner frequency) with about 60 m spacing. Over the 15 day deployment, the array recorded over 6,000 active source signals (vibroseis sweeps) and ambient noise that was dominated by traffic noise.We adopted the one-bit method to better reduce the effect of the active source. Spectral whitening was performed between 0.5 and 2 Hz. The continuous record was chopped into 1 minute segments. The 1-minute cross-correlation functions were initially stacked linearly, and then the phase-weighted stacking method was applied to improve signal quality. More than two million noise correlation functions (NCFs) have been obtained.The Rayleigh wave group velocity was measured on the symmetric component of the NCFs with the frequency-time analysis method. The average group velocity is about 400 m/s at 4 Hz, which is consistent with preliminary active source result. To avoid mis-picking possible precursors, the arrival time was picked at the peak in a two-second time window predicted with the average group velocity of the fundamental mode. The quality of the arrival measurements is defined by the signal-to-noise ratio. We were able to pick reliable arrivals at about 35% of the station-pairs. Since the straight-ray assumption may not be valid in a strongly heterogeneous medium, the wave path was traced with a finite difference scheme and the LSQR method was utilized to invert group velocity. The heterogeneous features of the group velocity map are consistent with a local geologic map. The PoroTomo project is funded by a grant from the U.S. Department of Energy.

Do Circumnuclear Dense Gas Disks Drive Mass Accretion onto Supermassive Black Holes?

NASA Astrophysics Data System (ADS)

Izumi, Takuma; Kawakatu, Nozomu; Kohno, Kotaro

2016-08-01

We present a positive correlation between the mass of dense molecular gas ({M}{{dense}}) of ˜100 pc scale circumnuclear disks (CNDs) and the black hole mass accretion rate ({\\dot{M}}{{BH}}) in a total of 10 Seyfert galaxies, based on data compiled from the literature and an archive (median aperture θ med = 220 pc). A typical {M}{{dense}} of CNDs is 107-8 {M}⊙ , estimated from the luminosity of the dense gas tracer, the HCN(1-0) emission line. Because dense molecular gas is the site of star formation, this correlation is virtually equivalent to the one between the nuclear star-formation rate and {\\dot{M}}{{BH}} revealed previously. Moreover, the {M}{{dense}}{--}{\\dot{M}}{{BH}} correlation was tighter for CND-scale gas than for the gas on kiloparsec or larger scales. This indicates that CNDs likely play an important role in fueling black holes, whereas greater than kiloparesec scale gas does not. To demonstrate a possible approach for studying the CND-scale accretion process with the Atacama Large Millimeter/submillimeter Array, we used a mass accretion model where angular momentum loss due to supernova explosions is vital. Based on the model prediction, we suggest that only the partial fraction of the mass accreted from the CND ({\\dot{M}}{{acc}}) is consumed as {\\dot{M}}{{BH}}. However, {\\dot{M}}{{acc}} agrees well with the total nuclear mass flow rate (I.e., {\\dot{M}}{{BH}} + outflow rate). Although these results are still tentative with large uncertainties, they support the view that star formation in CNDs can drive mass accretion onto supermassive black holes in Seyfert galaxies.

Improving Impedance of Implantable Microwire Multi-Electrode Arrays by Ultrasonic Electroplating of Durable Platinum Black

PubMed Central

Desai, Sharanya Arcot; Rolston, John D.; Guo, Liang; Potter, Steve M.

2010-01-01

Implantable microelectrode arrays (MEAs) have been a boon for neural stimulation and recording experiments. Commercially available MEAs have high impedances, due to their low surface area and small tip diameters, which are suitable for recording single unit activity. Lowering the electrode impedance, but preserving the small diameter, would provide a number of advantages, including reduced stimulation voltages, reduced stimulation artifacts and improved signal-to-noise ratio. Impedance reductions can be achieved by electroplating the MEAs with platinum (Pt) black, which increases the surface area but has little effect on the physical extent of the electrodes. However, because of the low durability of Pt black plating, this method has not been popular for chronic use. Sonicoplating (i.e. electroplating under ultrasonic agitation) has been shown to improve the durability of Pt black on the base metals of macro-electrodes used for cyclic voltammetry. This method has not previously been characterized for MEAs used in chronic neural implants. We show here that sonicoplating can lower the impedances of microwire multi-electrode arrays (MMEA) by an order of magnitude or more (depending on the time and voltage of electroplating), with better durability compared to pulsed plating or traditional DC methods. We also show the improved stimulation and recording performance that can be achieved in an in vivo implantation study with the sonicoplated low-impedance MMEAs, compared to high-impedance unplated electrodes. PMID:20485478

Solo but Not Separate: Preparing 21st-Century School Library Professionals Who Can "Go It Alone"

ERIC Educational Resources Information Center

Pasco, Becky

2011-01-01

Preparing school librarians for a diverse array of 21st-century educational environments is a daunting task. Faculty in school library preparation programs send candidates out into sparsely populated rural areas, dense urban settings, and everything in between. Some candidates will provide services and resources in updated, modern facilities,…

Flexible inorganic light emitting diodes and transparent PEDOT:PSS/Parylene C for simultaneous optogenetics and electrocorticography (Conference Presentation)

NASA Astrophysics Data System (ADS)

Lee, Keundong; Ganji, Mehran; Hossain, Lorraine; Ro, Yun Goo; Lee, Sang Heon; Park, Jong-woo; Yoo, Dongha; Yoon, Jiyoung; Yi, Gyu-Chul; Dayeh, Shadi A.

2017-02-01

Electrocorticography (ECoG) is a powerful tool for direct mapping of local field potentials from the brain surface. Progress in development of high-fidelity materials such as poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) on thin conformal substrates such as parylene C enabled intimate contact with cortical surfaces and higher quality recordings from small volumes of neurons. Meanwhile, stimulation of neuronal activity is conventionally accomplished with electrical microstimulation and transcranial magnetic stimulation that can be combined with ECoG to form the basis of bidirectional neural interface. However, these stimulation mechanisms are less controlled and primitively understood on the local and cellular levels. With the advent of optogenetics, the localization and specificity of neuronal stimulation and inhibition is possible. Therefore, the development of integrated devices that can merge the sensitivity of ECoG or depth recording with optogenetic tools can lead to newer frontiers in understanding the neuronal activity. Herein, we introduce a hybrid device comprising flexible inorganic LED arrays integrated PEDOT:PSS/parylene C microelectrode arrays for high resolution bidirectional neuronal interfaces. The flexible inorganic LEDs have been developed by the metal-organic vapor phase epitaxy of position-controlled GaN microLEDs on ZnO nanostructured templates pre-grown at precise locations on a graphene layer. By transferring it onto the microelectrode arrays, it can provides the individual electrical addressability by light stimulation patterns. We will present experimental and simulation results on the optoelectronic characteristics and light activation capability of flexible microLEDs and their evaluation in vivo.

Assessing the detection capability of a dense infrasound network in the southern Korean Peninsula

NASA Astrophysics Data System (ADS)

Che, Il-Young; Le Pichon, Alexis; Kim, Kwangsu; Shin, In-Cheol

2017-08-01

The Korea Infrasound Network (KIN) is a dense seismoacoustic array network consisting of eight small-aperture arrays with an average interarray spacing of ∼100 km. The processing of the KIN historical recordings over 10 yr in the 0.05-5 Hz frequency band shows that the dominant sources of signals are microbaroms and human activities. The number of detections correlates well with the seasonal and daily variability of the stratospheric wind dynamics. The quantification of the spatiotemporal variability of the KIN detection performance is simulated using a frequency-dependent semi-empirical propagation modelling technique. The average detection thresholds predicted for the region of interest by using both the KIN arrays and the International Monitoring System (IMS) infrasound station network at a given frequency of 1.6 Hz are estimated to be 5.6 and 10.0 Pa for two- and three-station coverage, respectively, which was about three times lower than the thresholds predicted by using only the IMS stations. The network performance is significantly enhanced from May to August, with detection thresholds being one order of magnitude lower than the rest of the year due to prevailing steady stratospheric winds. To validate the simulations, the amplitudes of ground-truth repeated surface mining explosions at an open-pit limestone mine were measured over a 19-month period. Focusing on the spatiotemporal variability of the stratospheric winds which control to first order where infrasound signals are expected to be detected, the predicted detectable signal amplitude at the mine and the detection capability at one KIN array located at a distance of 175 km are found to be in good agreement with the observations from the measurement campaign. The detection threshold in summer is ∼2 Pa and increases up to ∼300 Pa in winter. Compared with the low and stable thresholds in summer, the high temporal variability of the KIN performance is well predicted throughout the year. Simulations show that the performance of the global infrasound network of the IMS is significantly improved by adding KIN. This study shows the usefulness of dense regional networks to enhance detection capability in regions of interest in the context of future verification of the Comprehensive Nuclear-Test-Ban Treaty.

The effect of playing advergames that promote energy-dense snacks or fruit on actual food intake among children.

PubMed

Folkvord, Frans; Anschütz, Doeschka J; Buijzen, Moniek; Valkenburg, Patti M

2013-02-01

Previous studies have focused on the effects of television advertising on the energy intake of children. However, the rapidly changing food-marketing landscape requires research to measure the effects of nontraditional forms of marketing on the health-related behaviors of children. The main aim of this study was to examine the effect of advergames that promote energy-dense snacks or fruit on children's ad libitum snack and fruit consumption and to examine whether this consumption differed according to brand and product type (energy-dense snacks and fruit). The second aim was to examine whether advergames can stimulate fruit intake. We used a randomized between-subject design with 270 children (age: 8-10 y) who played an advergame that promoted energy-dense snacks (n = 69), fruit (n = 67), or nonfood products (n = 65) or were in the control condition (n = 69). Subsequently, we measured the free intake of energy-dense snacks and fruit. The children then completed questionnaire measures, and we weighed and measured them. The main finding was that playing an advergame containing food cues increased general energy intake, regardless of the advertised brand or product type (energy-dense snacks or fruit), and this activity particularly increased the intake of energy-dense snack foods. Children who played the fruit version of the advergame did not eat significantly more fruit than did those in the other groups. The findings suggest that playing advergames that promote food, including either energy-dense snacks or fruit, increases energy intake in children.

In vitro biocompatibility and electrical stability of thick-film platinum/gold alloy electrodes printed on alumina

NASA Astrophysics Data System (ADS)

Carnicer-Lombarte, Alejandro; Lancashire, Henry T.; Vanhoestenberghe, Anne

2017-06-01

Objective. High-density electrode arrays are a powerful tool in both clinical neuroscience and basic research. However, current manufacturing techniques require the use of specialised techniques and equipment, which are available to few labs. We have developed a high-density electrode array with customisable design, manufactured using simple printing techniques and with commercially available materials. Approach. Electrode arrays were manufactured by thick-film printing a platinum-gold alloy (Pt/Au) and an insulating dielectric on 96% alumina ceramic plates. Arrays were conditioned in serum and serum-free conditions, with and without 1 kHz, 200 µA, charge balanced stimulation for up to 21 d. Array biocompatibility was assessed using an extract assay and a PC-12 cell contact assay. Electrode impedance, charge storage capacity and charge injection capacity were before and after array conditioning. Main results. The manufactured Pt/Au electrodes have a highly porous surface and exhibit electrical properties comparable to arrays manufactured using alternative techniques. Materials used in array manufacture were found to be non-toxic to L929 fibroblasts by extract assay, and neuronal-like PC-12 cells adhered and extended neurites on the array surfaces. Arrays remained functional after long-term delivery of electrical pulses while exposed to protein-rich environments. Charge storage capacities and charge injection capacities increased following stimulation accounted for by an increase in surface index (real surface area) observed by vertical scanning interferometry. Further, we observed accumulation of proteins at the electrode sites following conditioning in the presence of serum. Significance. This study demonstrates the in vitro biocompatibility of commercially available thick-film printing materials. The printing technique is both simple and versatile, with layouts readily modified to produce customized electrode arrays. Thick-film electrode arrays are an attractive tool that may be implemented for general tissue engineering and neuroscience research.

A Hermetic Wireless Subretinal Neurostimulator for Vision Prostheses

PubMed Central

Shire, Douglas B.; Chen, Jinghua; Doyle, Patrick; Gingerich, Marcus D.; Cogan, Stuart F.; Drohan, William A.; Behan, Sonny; Theogarajan, Luke; Wyatt, John L.; Rizzo, Joseph F.

2016-01-01

A miniaturized, hermetically encased, wirelessly operated retinal prosthesis has been developed for preclinical studies in the Yucatan minipig, and includes several design improvements over our previously reported device. The prosthesis attaches conformally to the outside of the eye and electrically drives a microfabricated thin-film polyimide array of sputtered iridium oxide film electrodes. This array is implanted into the subretinal space using a customized ab externo surgical technique. The implanted device includes a hermetic titanium case containing a 15-channel stimulator chip and discrete circuit components. Feedthroughs in the case connect the stimulator chip to secondary power and data receiving coils on the eye and to the electrode array under the retina. Long-term in vitro pulse testing of the electrodes projected a lifetime consistent with typical devices in industry. The final assembly was tested in vitro to verify wireless operation of the system in physiological saline using a custom RF transmitter and primary coils. Stimulation pulse strength, duration, and frequency were programmed wirelessly from a Peripheral Component Interconnect eXtensions for Instrumentation (PXI) computer. Operation of the retinal implant has been verified in two pigs for up to five and a half months by detecting stimulus artifacts generated by the implanted device. PMID:21859595

A CMOS active pixel sensor for retinal stimulation

NASA Astrophysics Data System (ADS)

Prydderch, Mark L.; French, Marcus J.; Mathieson, Keith; Adams, Christopher; Gunning, Deborah; Laudanski, Jonathan; Morrison, James D.; Moodie, Alan R.; Sinclair, James

2006-02-01

Degenerative photoreceptor diseases, such as age-related macular degeneration and retinitis pigmentosa, are the most common causes of blindness in the western world. A potential cure is to use a microelectronic retinal prosthesis to provide electrical stimulation to the remaining healthy retinal cells. We describe a prototype CMOS Active Pixel Sensor capable of detecting a visual scene and translating it into a train of electrical pulses for stimulation of the retina. The sensor consists of a 10 x 10 array of 100 micron square pixels fabricated on a 0.35 micron CMOS process. Light incident upon each pixel is converted into output current pulse trains with a frequency related to the light intensity. These outputs are connected to a biocompatible microelectrode array for contact to the retinal cells. The flexible design allows experimentation with signal amplitudes and frequencies in order to determine the most appropriate stimulus for the retina. Neural processing in the retina can be studied by using the sensor in conjunction with a Field Programmable Gate Array (FPGA) programmed to behave as a neural network. The sensor has been integrated into a test system designed for studying retinal response. We present the most recent results obtained from this sensor.

Bubble Transport through Micropillar Arrays

NASA Astrophysics Data System (ADS)

Lee, Kenneth; Savas, Omer

2012-11-01

In current energy research, artificial photosynthetic devices are being designed to split water and harvest hydrogen gas using energy from the sun. In one such design, hydrogen gas bubbles evolve on the catalytic surfaces of arrayed micropillars. If these bubbles are not promptly removed from the surface, they can adversely affect gas evolution rates, water flow rates, sunlight capture, and heat management of the system. Therefore, an efficient method of collecting the evolved gas bubbles is crucial. Preliminary flow visualization has been conducted of bubbles advecting through dense arrays of pillars. Bubbles moving through square and hexagonal arrays are tracked, and the results are qualitatively described. Initial attempts to correlate bubble motion with relevant lengthscales and forces are also presented. These observations suggest how bubble transport within such pillar arrays can be managed, as well as guide subsequent experiments that investigate bubble evolution and collection. This material is based upon work performed by the Joint Center for Artificial Photosynthesis, a DOE Energy Innovation Hub, supported through the Office of Science of the U.S. Department of Energy under Award Number DE-SC0004993.

A simple and transparent well-aligned ZnO nanowire array ultraviolet photodetector with high responsivity

NASA Astrophysics Data System (ADS)

Yin, Lei; Ding, Hesheng; Yuan, Zhaolin; Huang, Wendeng; Shuai, Chunjiang; Xiong, Zhaoxin; Deng, Jianping; Lv, Tengbo

2018-06-01

Well-aligned zinc oxide (ZnO) nanowire arrays were grown on an interdigital patterned fluorine tin oxide (FTO)-coated glass substrate by a facile chemical bath deposition at low temperature. Morphology, crystalline structure, and optical properties of the ZnO nanowire arrays were analyzed in detail. The results revealed that the ZnO nanowires had wurtzite structure, typically ∼40-60 nm in diameter, and ∼700-800 nm in length, a great number of highly uniform and dense nanowires grew vertically on the substrate to form the well-aligned ZnO nanowire arrays, which had very high optical transmission (>86%) in the visible light region. In addition, the performance of ZnO nanowire arrays ultraviolet (UV) photodetector was systematically examined. The photosensitivity (S), responsivity (R), response and decay time of the photodetector were 703 at +0.2 V, 113 A/W at +5 V, 23 s and 73 s respectively. Also, the photoresponse mechanism of the UV photodetector was illuminated in terms of the oxygen adsorption-photodesorption process.

Silicon nanowire arrays as thermoelectric material for a power microgenerator

NASA Astrophysics Data System (ADS)

Dávila, D.; Tarancón, A.; Fernández-Regúlez, M.; Calaza, C.; Salleras, M.; San Paulo, A.; Fonseca, L.

2011-10-01

A novel design of a silicon-based thermoelectric power microgenerator is presented in this work. Arrays of silicon nanowires, working as thermoelectric material, have been integrated in planar uni-leg thermocouple microstructures to convert waste heat into electrical energy. Homogeneous, uniformly dense, well-oriented and size-controlled arrays of silicon nanowires have been grown by chemical vapor deposition using the vapor-liquid-solid mechanism. Compatibility issues between the nanowire growth method and microfabrication techniques, such as electrical contact patterning, are discussed. Electrical measurements of the nanowire array electrical conductivity and the Seebeck voltage induced by a controlled thermal gradient or under harvesting operation mode have been carried out to demonstrate the feasibility of the microdevice. A resistance of 240 Ω at room temperature was measured for an array of silicon nanowires 10 µm -long, generating a Seebeck voltage of 80 mV under an imposed thermal gradient of 450 °C, whereas only 4.5 mV were generated under a harvesting operation mode. From the results presented, a Seebeck coefficient of about 150-190 µV K-1 was estimated, which corresponds to typical values for bulk silicon.

Coronal plasma development in wire-array z-pinches made of twisted-pairs

NASA Astrophysics Data System (ADS)

Hoyt, C. L.; Greenly, J. B.; Gourdain, P. A.; Knapp, P. F.; Pikuz, S. A.; Shelkovenko, T. A.; Hammer, D. A.; Kusse, B. R.

2009-11-01

We have investigated coronal and core plasma development in wire array z-pinches in which single fine wires are replaced by twisted-pairs (``cable'') on the 1 MA, 100 ns rise time COBRA pulsed power generator. X-ray radiography, employed to investigate dense wire core expansion, showed periodic axial nonuniformity and evidence for shock waves developing where the individual wire plasmas collide. Laser shadowgraphy images indicated that the axial instability properties of the coronal plasma are substantially modified from ordinary wire arrays. Cable mass per unit length, material and the twist wavelength were varied in order to study their effects upon the instability wavelength. Implosion uniformity and bright-spot formation, as well as magnetic topology evolution, have also been investigated using self-emission imaging, x-ray diagnostics and small B-dot probes, respectively. Results from the cable-array z-pinches will be compared with results from ordinary wire-array z-pinches. This research was supported by the SSAA program of the National Nuclear Security Administration under DOE Cooperative agreement DE-FC03-02NA00057.

Neuromuscular response amplitude to mechanical stimulation using large-array surface electromyography in participants with and without chronic low back pain.

PubMed

Pagé, Isabelle; Nougarou, François; Descarreaux, Martin

2016-04-01

The present study aimed to compare the neuromuscular response under various mechanical stimulations of the lumbar spine in participants with and without chronic low back pain (cLBP). Four mechanical stimulations, characterized by forces ranging from 75 to 225N, were delivered using a servo-controlled linear actuator motor to the L3 spinous process of 25 healthy participants and 26 participants with cLBP. Lumbar neuromuscular responses were recorded using 64-electrodes large surface electromyography arrays. Between-group differences in the dose-response relationship (neuromuscular response amplitude according to each force level) were assessed using mixed model ANOVAs. No differences between groups were shown (all p values>.05). A significant linear relationship was observed between forces and neuromuscular response amplitudes (p<.001) indicating an increase in response amplitudes with increasing stimulation force. Responses were observed throughout the lumbar region with highest response amplitudes in the vicinity of the contacted vertebra. The neuromuscular response amplitude triggered by localized lumbar mechanical stimulations does not differ between participants with and without cLBP. Moreover, even though stimulations were delivered at specific spinal segment, a neuromuscular response, although rapidly decreasing, was observed in areas distant from the contact site. Copyright © 2016 Elsevier Ltd. All rights reserved.

How Learning Environments Can Stimulate Student Imagination

ERIC Educational Resources Information Center

Liang, Chaoyun; Hsu, Yuling; Huang, Yinghsiu; Chen, Sheng-Chih

2012-01-01

The purpose of this study was to investigate an array of environmental factors that can stimulate imagination and explore how these factors manifest in different design phases. The participants of this study were students in the field of educational technology from four universities across Taiwan. The instructional design process was divided into…

Making Carbon-Nanotube Arrays Using Block Copolymers: Part 2

NASA Technical Reports Server (NTRS)

Bronikowski, Michael

2004-01-01

Some changes have been incorporated into a proposed method of manufacturing regular arrays of precisely sized, shaped, positioned, and oriented carbon nanotubes. Such arrays could be useful as mechanical resonators for signal filters and oscillators, and as electrophoretic filters for use in biochemical assays. A prior version of the method was described in Block Copolymers as Templates for Arrays of Carbon Nanotubes, (NPO-30240), NASA Tech Briefs, Vol. 27, No. 4 (April 2003), page 56. To recapitulate from that article: As in other previously reported methods, carbon nanotubes would be formed by decomposition of carbon-containing gases over nanometer-sized catalytic metal particles that had been deposited on suitable substrates. Unlike in other previously reported methods, the catalytic metal particles would not be so randomly and densely distributed as to give rise to thick, irregular mats of nanotubes with a variety of lengths, diameters, and orientations. Instead, in order to obtain regular arrays of spaced-apart carbon nanotubes as nearly identical as possible, the catalytic metal particles would be formed in predetermined regular patterns with precise spacings. The regularity of the arrays would be ensured by the use of nanostructured templates made of block copolymers.

Nanoscale neuroelectronic interface based on open-ended nanocoax arrays

NASA Astrophysics Data System (ADS)

Naughton, Jeffrey R.; Rizal, Binod; Burns, Michael J.; Yeom, Jee; Heyse, Shannon; Archibald, Michelle; Shepard, Stephen; McMahon, Gregory; Chiles, Thomas C.; Naughton, Michael J.

2012-02-01

We describe the development of a nanoscale neuroelectronic array with submicron pixelation for recording and stimulation with high spatial resolution. The device is composed of an array of nanoscale coaxial electrodes, either network- or individually-configured. As a neuroelectronic interface, it will employ noninvasive real-time capacitive coupling to the plasma membrane with potential for extracellular recording of intra- and interneural synaptic activity, with one target being precision measurement of electrical signals associated with induced and spontaneous synapse firing in pre- and post-synaptic somata. Subarrays or even individual pixels can also be actuated for precisely-localized stimulation. We report initial results from measurements using the rat adrenal pheochromocytoma PC12 cell line, which terminally differentiates in response to nerve growth factor, as well as SH-SY5Y neuroblastoma cells in response to retinoic acid, characterizing the basic performance of the fabricated device.

Nanostructured cavity devices for extracellular stimulation of HL-1 cells

NASA Astrophysics Data System (ADS)

Czeschik, Anna; Rinklin, Philipp; Derra, Ulrike; Ullmann, Sabrina; Holik, Peter; Steltenkamp, Siegfried; Offenhäusser, Andreas; Wolfrum, Bernhard

2015-05-01

Microelectrode arrays (MEAs) are state-of-the-art devices for extracellular recording and stimulation on biological tissue. Furthermore, they are a relevant tool for the development of biomedical applications like retina, cochlear and motor prostheses, cardiac pacemakers and drug screening. Hence, research on functional cell-sensor interfaces, as well as the development of new surface structures and modifications for improved electrode characteristics, is a vivid and well established field. However, combining single-cell resolution with sufficient signal coupling remains challenging due to poor cell-electrode sealing. Furthermore, electrodes with diameters below 20 µm often suffer from a high electrical impedance affecting the noise during voltage recordings. In this study, we report on a nanocavity sensor array for voltage-controlled stimulation and extracellular action potential recordings on cellular networks. Nanocavity devices combine the advantages of low-impedance electrodes with small cell-chip interfaces, preserving a high spatial resolution for recording and stimulation. A reservoir between opening aperture and electrode is provided, allowing the cell to access the structure for a tight cell-sensor sealing. We present the well-controlled fabrication process and the effect of cavity formation and electrode patterning on the sensor's impedance. Further, we demonstrate reliable voltage-controlled stimulation using nanostructured cavity devices by capturing the pacemaker of an HL-1 cell network.Microelectrode arrays (MEAs) are state-of-the-art devices for extracellular recording and stimulation on biological tissue. Furthermore, they are a relevant tool for the development of biomedical applications like retina, cochlear and motor prostheses, cardiac pacemakers and drug screening. Hence, research on functional cell-sensor interfaces, as well as the development of new surface structures and modifications for improved electrode characteristics, is a vivid and well established field. However, combining single-cell resolution with sufficient signal coupling remains challenging due to poor cell-electrode sealing. Furthermore, electrodes with diameters below 20 µm often suffer from a high electrical impedance affecting the noise during voltage recordings. In this study, we report on a nanocavity sensor array for voltage-controlled stimulation and extracellular action potential recordings on cellular networks. Nanocavity devices combine the advantages of low-impedance electrodes with small cell-chip interfaces, preserving a high spatial resolution for recording and stimulation. A reservoir between opening aperture and electrode is provided, allowing the cell to access the structure for a tight cell-sensor sealing. We present the well-controlled fabrication process and the effect of cavity formation and electrode patterning on the sensor's impedance. Further, we demonstrate reliable voltage-controlled stimulation using nanostructured cavity devices by capturing the pacemaker of an HL-1 cell network. Electronic supplementary information (ESI) available: Comparison of non-filtered and Savitzky-Golay filtered action potential recordings, electrical signals and corresponding optical signals. See DOI: 10.1039/c5nr01690h

Source characterization of a small earthquake cluster at Edmond, Oklahoma using a very dense array

NASA Astrophysics Data System (ADS)

Ng, R.; Nakata, N.

2017-12-01

Recent seismicity in Oklahoma has caught the attention of the public in the last few years since seismicity is commonly related to loss in urban areas. To account for the increase in public interest, improve the understanding of damaging ground motions produced in earthquakes and develop better seismic hazard assessment, we must characterize the seismicity in Oklahoma and its associated structure and source parameters. Regional changes in subsurface stresses have increased seismic activities due to reactivation of faults in places such as central Oklahoma. It is imperative for seismic investigation and modeling to characterize subsurface structural features that may influence the damaging effects of ground motion. We analyze the full-waveform data collected from a temporary dense array of 72 portable seismometers with a 110 meter spacing that were active for a one-month period from May to June 2017, deployed at Edmond, Oklahoma. The data from this one-month duration array captured over 10,000 events and enabled us to make measurements of small-scale lateral variations of earthquake wavefields. We examine the waveform for events using advanced methods of detection, location and determine the source mechanism. We compare our results with selected events listed in the Oklahoma Geological Survey (OGS) and United States Geological Survey (USGS) catalogue. Based on the detection and located small events, we will discuss the causative fault structure at the area and present the results of the investigation.

Detection of small earthquakes with dense array data: example from the San Jacinto fault zone, southern California

NASA Astrophysics Data System (ADS)

Meng, Haoran; Ben-Zion, Yehuda

2018-01-01

We present a technique to detect small earthquakes not included in standard catalogues using data from a dense seismic array. The technique is illustrated with continuous waveforms recorded in a test day by 1108 vertical geophones in a tight array on the San Jacinto fault zone. Waveforms are first stacked without time-shift in nine non-overlapping subarrays to increase the signal-to-noise ratio. The nine envelope functions of the stacked records are then multiplied with each other to suppress signals associated with sources affecting only some of the nine subarrays. Running a short-term moving average/long-term moving average (STA/LTA) detection algorithm on the product leads to 723 triggers in the test day. Using a local P-wave velocity model derived for the surface layer from Betsy gunshot data, 5 s long waveforms of all sensors around each STA/LTA trigger are beamformed for various incident directions. Of the 723 triggers, 220 are found to have localized energy sources and 103 of these are confirmed as earthquakes by verifying their observation at 4 or more stations of the regional seismic network. This demonstrates the general validity of the method and allows processing further the validated events using standard techniques. The number of validated events in the test day is >5 times larger than that in the standard catalogue. Using these events as templates can lead to additional detections of many more earthquakes.

Near-field observations of microearthquake source physics using dense array

NASA Astrophysics Data System (ADS)

Chen, X.; Nakata, N.; Abercrombie, R. E.

2017-12-01

The recorded waveform includes contributions from earthquake source properties and propagation effects, leading to long-standing trade-off problems between site/path effects and source effects. This problem is especially significant for small earthquakes where the corner frequencies are within similar ranges of near-site attenuation effects. Fortunately, this problem can be remedied by dense near-field recordings at high frequency, and large databases with wide magnitude range. The 2016 IRIS wavefield experiment provides high-quality recordings of earthquake sequences in north-central Oklahoma with about 400 sensors in 15 km area. Preliminary processing of the IRIS wavefield array resulted with about 20,000 microearthquakes ranging from M-1 to M2, while only 2 earthquakes are listed in the catalog during the same time period. A preliminary examination of the catalog reveals three similar magnitude earthquakes (M 2) occurred at similar locations within 9 seconds of each other. Utilizing this catalog, we will combine individual empirical Green's function (EGF) analysis and stacking over multiple EGFs to examine if there are any systematic variations of source time functions and spectral ratios across the array, which will provide constrains of rupture complexity, directivity and earthquake interactions. For example, this would help us to understand if these three earthquakes rupture overlapping fault patches from cascading failure, or from repeated rupture at the same slip patch due to external stress loading. Deciphering the interaction at smaller scales with near-field observations is important for a controlled earthquake experiment.

Basic data features and results from a spatially dense seismic array on the San Jacinto fault zone

NASA Astrophysics Data System (ADS)

Ben-Zion, Yehuda; Vernon, Frank L.; Ozakin, Yaman; Zigone, Dimitri; Ross, Zachary E.; Meng, Haoran; White, Malcolm; Reyes, Juan; Hollis, Dan; Barklage, Mitchell

2015-07-01

We discuss several outstanding aspects of seismograms recorded during >4 weeks by a spatially dense Nodal array, straddling the damage zone of the San Jacinto fault in southern California, and some example results. The waveforms contain numerous spikes and bursts of high-frequency waves (up to the recorded 200 Hz) produced in part by minute failure events in the shallow crust. The high spatial density of the array facilitates the detection of 120 small local earthquakes in a single day, most of which not detected by the surrounding ANZA and regional southern California networks. Beamforming results identify likely ongoing cultural noise sources dominant in the frequency range 1-10 Hz and likely ongoing earthquake sources dominant in the frequency range 20-40 Hz. Matched-field processing and back-projection of seismograms provide alternate event location. The median noise levels during the experiment at different stations, waves generated by Betsy gunshots, and wavefields from nearby earthquakes point consistently to several structural units across the fault. Seismic trapping structure and local sedimentary basin produce localized motion amplification and stronger attenuation than adjacent regions. Cross correlations of high-frequency noise recorded at closely spaced stations provide a structural image of the subsurface material across the fault zone. The high spatial density and broad frequency range of the data can be used for additional high resolution studies of structure and source properties in the shallow crust.

Dense Array Studies of Volcano-Tectonic and Long-Period Earthquakes Beneath Mount St. Helens

NASA Astrophysics Data System (ADS)

Glasgow, M. E.; Hansen, S. M.; Schmandt, B.; Thomas, A.

2017-12-01

A 904 single-component 10-Hz geophone array deployed within 15 km of Mount St. Helens (MSH) in 2014 recorded continuously for two-weeks. Automated reverse-time imaging (RTI) was used to generate a catalog of 212 earthquakes. Among these, two distinct types of upper crustal (<8 km) earthquakes were classified. Volcano-tectonic (VT) and long-period (LP) earthquakes were identified using analysis of array spectrograms, envelope functions, and velocity waveforms. To remove analyst subjectivity, quantitative classification criteria were developed based on the ratio of power in high and low frequency bands and coda duration. Prior to the 2014 experiment, upper crustal LP earthquakes had only been reported at MSH during volcanic activity. Subarray beamforming was used to distinguish between LP earthquakes and surface generated LP signals, such as rockfall. This method confirmed 16 LP signals with horizontal velocities exceeding that of upper crustal P-wave velocities, which requires a subsurface hypocenter. LP and VT locations overlap in a cluster slightly east of the summit crater from 0-5 km below sea level. LP displacement spectra are similar to simple theoretical predictions for shear failure except that they have lower corner frequencies than VT earthquakes of similar magnitude. The results indicate a distinct non-resonant source for LP earthquakes which are located in the same source volume as some VT earthquakes (within hypocenter uncertainty of 1 km or less). To further investigate MSH microseismicity mechanisms, a 142 three-component (3-C) 5 Hz geophone array will record continuously for one month at MSH in Fall 2017 providing a unique dataset for a volcano earthquake source study. This array will help determine if LP occurrence in 2014 was transient or if it is still ongoing. Unlike the 2014 array, approximately 50 geophones will be deployed in the MSH summit crater directly over the majority of seismicity. RTI will be used to detect and locate earthquakes by back-projecting 3-C data with a local 3-D P and S velocity model. Earthquakes will be classified using the previously stated techniques, and we will seek to use the dense array of 3-C waveforms to invert for focal mechanisms and, ideally, moment tensor sources down to M0.

Enhancing long-term memory with stimulation tunes visual attention in one trial.

PubMed

Reinhart, Robert M G; Woodman, Geoffrey F

2015-01-13

Scientists have long proposed that memory representations control the mechanisms of attention that focus processing on the task-relevant objects in our visual field. Modern theories specifically propose that we rely on working memory to store the object representations that provide top-down control over attentional selection. Here, we show that the tuning of perceptual attention can be sharply accelerated after 20 min of noninvasive brain stimulation over medial-frontal cortex. Contrary to prevailing theories of attention, these improvements did not appear to be caused by changes in the nature of the working memory representations of the search targets. Instead, improvements in attentional tuning were accompanied by changes in an electrophysiological signal hypothesized to index long-term memory. We found that this pattern of effects was reliably observed when we stimulated medial-frontal cortex, but when we stimulated posterior parietal cortex, we found that stimulation directly affected the perceptual processing of the search array elements, not the memory representations providing top-down control. Our findings appear to challenge dominant theories of attention by demonstrating that changes in the storage of target representations in long-term memory may underlie rapid changes in the efficiency with which humans can find targets in arrays of objects.

Seismicity and Crustal Anisotropy Beneath the Western Segment of the North Anatolian Fault: Results from a Dense Seismic Array

NASA Astrophysics Data System (ADS)

Turkelli, N.; Teoman, U.; Altuncu Poyraz, S.; Cambaz, D.; Mutlu, A. K.; Kahraman, M.; Houseman, G. A.; Rost, S.; Thompson, D. A.; Cornwell, D. G.; Utkucu, M.; Gülen, L.

2013-12-01

The North Anatolian Fault (NAF) is one of the major strike slip fault systems on Earth comparable to San Andreas Fault in some ways. Devastating earthquakes have occurred along this system causing major damage and casualties. In order to comprehensively investigate the shallow and deep crustal structure beneath the western segment of NAF, a temporary dense seismic network for North Anatolia (DANA) consisting of 73 broadband sensors was deployed in early May 2012 surrounding a rectangular grid of by 70 km and a nominal station spacing of 7 km with the aim of further enhancing the detection capability of this dense seismic array. This joint project involves researchers from University of Leeds, UK, Bogazici University Kandilli Observatory and Earthquake Research Institute (KOERI), and University of Sakarya and primarily focuses on upper crustal studies such as earthquake locations (especially micro-seismic activity), receiver functions, moment tensor inversions, shear wave splitting, and ambient noise correlations. To begin with, we obtained the hypocenter locations of local earthquakes that occured within the DANA network. The dense 2-D grid geometry considerably enhanced the earthquake detection capability which allowed us to precisely locate events with local magnitudes (Ml) less than 1.0. Accurate earthquake locations will eventually lead to high resolution images of the upper crustal structure beneath the northern and southern branches of NAF in Sakarya region. In order to put additional constraints on the active tectonics of the western part of NAF, we also determined fault plane solutions using Regional Moment Tensor Inversion (RMT) and P wave first motion methods. For the analysis of high quality fault plane solutions, data from KOERI and the DANA project were merged. Furthermore, with the aim of providing insights on crustal anisotropy, shear wave splitting parameters such as lag time and fast polarization direction were obtained for local events recorded within the seismic network with magnitudes larger than 2.5.

Integration of Organic Electrochemical and Field-Effect Transistors for Ultraflexible, High Temporal Resolution Electrophysiology Arrays.

PubMed

Lee, Wonryung; Kim, Dongmin; Rivnay, Jonathan; Matsuhisa, Naoji; Lonjaret, Thomas; Yokota, Tomoyuki; Yawo, Hiromu; Sekino, Masaki; Malliaras, George G; Someya, Takao

2016-11-01

Integration of organic electrochemical transistors and organic field-effect transistors is successfully realized on a 600 nm thick parylene film toward an electrophysiology array. A single cell of an integrated device and a 2 × 2 electrophysiology array succeed in detecting electromyogram with local stimulation of the motor nerve bundle of a transgenic rat by a laser pulse. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Large enhancement of X-ray excited luminescence in Ga-doped ZnO nanorod arrays by hydrogen annealing

NASA Astrophysics Data System (ADS)

Li, Qianli; Liu, Xiaoliln; Gu, Mu; Li, Fengrui; Zhang, Juannan; Wu, Qiang; Huang, Shiming; Liu, Si

2018-03-01

Highly c-axis oriented and densely packed ZnO:Ga nanorod arrays were fabricated on ZnO-seeded substrates by hydrothermal method, and the effect of hydrogen annealing on their morphology, structure and luminescence properties was investigated in detail. Under ultraviolet or X-ray excitation, an intense ultraviolet luminescence appeared in the hydrogen-annealed samples owing to the formation of a shallow hydrogen donor state, which can sharply activate the reconbination radiation. The luminescence intensity increased with the annealing temperature, and then decreased at a higher temperature due to the dissociation of the hydrogen ion. The optimum concentration and time of hydrogen annealing were acquired simultaneously. It is expected that the ZnO:Ga nanorod array is a promising candidate for application in ultrafast and high-spatial-resolution X-ray imaging detector.

Supercomputing on massively parallel bit-serial architectures

NASA Technical Reports Server (NTRS)

Iobst, Ken

1985-01-01

Research on the Goodyear Massively Parallel Processor (MPP) suggests that high-level parallel languages are practical and can be designed with powerful new semantics that allow algorithms to be efficiently mapped to the real machines. For the MPP these semantics include parallel/associative array selection for both dense and sparse matrices, variable precision arithmetic to trade accuracy for speed, micro-pipelined train broadcast, and conditional branching at the processing element (PE) control unit level. The preliminary design of a FORTRAN-like parallel language for the MPP has been completed and is being used to write programs to perform sparse matrix array selection, min/max search, matrix multiplication, Gaussian elimination on single bit arrays and other generic algorithms. A description is given of the MPP design. Features of the system and its operation are illustrated in the form of charts and diagrams.

Imaging with New Classic and Vision at the NPOI

NASA Astrophysics Data System (ADS)

Jorgensen, Anders

2018-04-01

The Navy Precision Optical Interferometer (NPOI) is unique among interferometric observatories for its ability to position telescopes in an equally-spaced array configuration. This configuration is optimal for interferometric imaging because it allows the use of bootstrapping to track fringes on long baselines with signal-to-noise ratio less than one. When combined with coherent integration techniques this can produce visibilities with acceptable SNR on baselines long enough to resolve features on the surfaces of stars. The stellar surface imaging project at NPOI combines the bootstrapping array configuration of the NPOI array, real-time fringe tracking, baseline- and wavelength bootstrapping with Earth rotation to provide dense coverage in the UV plane at a wide range of spatial frequencies. In this presentation, we provide an overview of the project and an update of the latest status and results from the project.

A thickness-mode piezoelectric micromachined ultrasound transducer annular array using a PMN–PZT single crystal

NASA Astrophysics Data System (ADS)

Kang, Woojin; Jung, Joontaek; Lee, Wonjun; Ryu, Jungho; Choi, Hongsoo

2018-07-01

Micro-electromechanical system (MEMS) technologies were used to develop a thickness-mode piezoelectric micromachined ultrasonic transducer (Tm-pMUT) annular array utilizing a lead magnesium niobate–lead zirconate titanate (PMN–PZT) single crystal prepared by the solid-state single-crystal-growth method. Dicing is a conventional processing method for PMN–PZT single crystals, but MEMS technology can be adopted for the development of Tm-pMUT annular arrays and has various advantages, including fabrication reliability, repeatability, and a curved element shape. An inductively coupled plasma–reactive ion etching process was used to etch a brittle PMN–PZT single crystal selectively. Using this process, eight ring-shaped elements were realized in an area of 1  ×  1 cm2. The resonance frequency and effective electromechanical coupling coefficient of the Tm-pMUT annular array were 2.66 (±0.04) MHz, 3.18 (±0.03) MHz, and 30.05%, respectively, in the air. The maximum positive acoustic pressure in water, measured at a distance of 7.27 mm, was 40 kPa from the Tm-pMUT annular array driven by a 10 Vpp sine wave at 2.66 MHz without beamforming. The proposed Tm-pMUT annular array using a PMN–PZT single crystal has the potential for various applications, such as a fingerprint sensor, and for ultrasonic cell stimulation and low-intensity tissue stimulation.

AAO-based nanoreservoir arrays: A quick and easy support for TEM characterization

NASA Astrophysics Data System (ADS)

Mace, M.; Sahaf, H.; Moyen, E.; Bedu, F.; Masson, L.; Hanbücken, M.

2010-12-01

Large-scale arrays of calibrated, nanometer sized reservoirs are prepared by adapting the well-established electrochemical method used so far for the preparation of anodic aluminium oxide (AAO) membranes. The bottom plane of the assembly is prepared to be transparent for high-energy electrons, enabling their use as a universal sample support for transmission electron microscopy studies of nanoparticles. The nanoreservoir substrates can be cleaned under ultra-high-vacuum conditions and filled, by evaporating different materials. Filled nanoreservoirs can locally be sealed with a thin carbon layer using focused-ion-beam-induced deposition (FIBID). Nanoparticles, grow at various adsorption places on the walls and bottom planes inside the nanoreservoirs. They can be characterized by transmission electron microscopy (TEM) without further sample preparation in different crystallographic directions. Due to the dense array-arrangement of the reservoirs, very good statistics can already be obtained on one single sample. The controlled fabrication of the nanoreservoir array and first TEM results obtained on Au nanoparticles before and after sealing of the reservoirs, are presented.

Performance bounds for modal analysis using sparse linear arrays

NASA Astrophysics Data System (ADS)

Li, Yuanxin; Pezeshki, Ali; Scharf, Louis L.; Chi, Yuejie

2017-05-01

We study the performance of modal analysis using sparse linear arrays (SLAs) such as nested and co-prime arrays, in both first-order and second-order measurement models. We treat SLAs as constructed from a subset of sensors in a dense uniform linear array (ULA), and characterize the performance loss of SLAs with respect to the ULA due to using much fewer sensors. In particular, we claim that, provided the same aperture, in order to achieve comparable performance in terms of Cramér-Rao bound (CRB) for modal analysis, SLAs require more snapshots, of which the number is about the number of snapshots used by ULA times the compression ratio in the number of sensors. This is shown analytically for the case with one undamped mode, as well as empirically via extensive numerical experiments for more complex scenarios. Moreover, the misspecified CRB proposed by Richmond and Horowitz is also studied, where SLAs suffer more performance loss than their ULA counterpart.

Estimation of electrode location in a rat motor cortex by laminar analysis of electrophysiology and intracortical electrical stimulation

NASA Astrophysics Data System (ADS)

Yazdan-Shahmorad, A.; Lehmkuhle, M. J.; Gage, G. J.; Marzullo, T. C.; Parikh, H.; Miriani, R. M.; Kipke, D. R.

2011-08-01

While the development of microelectrode arrays has enabled access to disparate regions of a cortex for neurorehabilitation, neuroprosthetic and basic neuroscience research, accurate interpretation of the signals and manipulation of the cortical neurons depend upon the anatomical placement of the electrode arrays in a layered cortex. Toward this end, this report compares two in vivo methods for identifying the placement of electrodes in a linear array spaced 100 µm apart based on in situ laminar analysis of (1) ketamine-xylazine-induced field potential oscillations in a rat motor cortex and (2) an intracortical electrical stimulation-induced movement threshold. The first method is based on finding the polarity reversal in laminar oscillations which is reported to appear at the transition between layers IV and V in laminar 'high voltage spindles' of the rat cortical column. Analysis of histological images in our dataset indicates that polarity reversal is detected 150.1 ± 104.2 µm below the start of layer V. The second method compares the intracortical microstimulation currents that elicit a physical movement for anodic versus cathodic stimulation. It is based on the hypothesis that neural elements perpendicular to the electrode surface are preferentially excited by anodic stimulation while cathodic stimulation excites those with a direction component parallel to its surface. With this method, we expect to see a change in the stimulation currents that elicits a movement at the beginning of layer V when comparing anodic versus cathodic stimulation as the upper cortical layers contain neuronal structures that are primarily parallel to the cortical surface and lower layers contain structures that are primarily perpendicular. Using this method, there was a 78.7 ± 68 µm offset in the estimate of the depth of the start of layer V. The polarity reversal method estimates the beginning of layer V within ±90 µm with 95% confidence and the intracortical stimulation method estimates it within ±69.3 µm. We propose that these methods can be used to estimate the in situ location of laminar electrodes implanted in the rat motor cortex.

Micromachined electrode array

DOEpatents

Okandan, Murat; Wessendorf, Kurt O.

2007-12-11

An electrode array is disclosed which has applications for neural stimulation and sensing. The electrode array, in certain embodiments, can include a plurality of electrodes each of which is flexibly attached to a common substrate using a plurality of springs to allow the electrodes to move independently. In other embodiments of the electrode array, the electrodes can be fixed to the substrate. The electrode array can be formed from a combination of bulk and surface micromachining, and can include electrode tips having an electroplated metal (e.g. platinum, iridium, gold or titanium) or a metal oxide (e.g. iridium oxide) for biocompatibility. The electrode array can be used to form a part of a neural prosthesis, and is particularly well adapted for use in an implantable retinal prosthesis.

Nonlinear Spectroscopy of Multicomponent Droplets and Two- and Three-Dimensional Measurements in Flames

DTIC Science & Technology

1989-01-27

carbon dioxide laser has caused the droplet to undergo vaporization. --- - -(tont-nued) 20. DISTRIBUTION /AVAILABIUTV OF ATRACT 21. ABSTRACT SECURITY...breakdown threshold and produced a dense, high temperature plasma which can abosrb the stimulated Raman radiation; (3) studies on the dependence of the

DEMONSTRATION OF BIODEGRADATION OF DENSE, NONAQUEOUS-PHASE LIQUIDS (DNAPL)THROUGH BIOSTIMULATION AND BIOAUGMENTATION AT LAUNCH COMPLEX 34 IN CAPE CANAVERAL AIR FORCE STATION, FLORIDA

EPA Science Inventory

Biostimulation involves stimulating indigenous microbial cultures by adding nutrients whereas bioaugmentation involves introducing microbial cultures that are particularly adept at degrading these contaminants into the target aquifer. This demonstration involved biostimulation fo...

Neural Activation Underlying Cognitive Control in the Context of Neutral and Affectively Charged Pictures in Children

ERIC Educational Resources Information Center

Lamm, Connie; White, Lauren K.; McDermott, Jennifer Martin; Fox, Nathan A.

2012-01-01

The neural correlates of cognitive control for typically developing 9-year-old children were examined using dense-array ERPs and estimates of cortical activation (LORETA) during a go/no-go task with two conditions: a neutral picture condition and an affectively charged picture condition. Activation was estimated for the entire cortex after which…

Evidence of the Dampening Effect of Dense E-region Structures on E-F Coupling

NASA Astrophysics Data System (ADS)

Helmboldt, J.

2012-12-01

Results from a combination of instruments including ionosondes, GPS receivers, the Very Large Array (VLA), and the Long Wavelength Array (LWA) are used to demonstrate the role structure within the E-region plays in coupling between instabilities within the E and F regions at midlatitudes. VLA observations of cosmic sources at 74 MHz during summer nighttime in 2002 detected northwest-to-southeast aligned wavefronts, consistent with medium-scale traveling ionospheric disturbances (MSTIDs). These waves were only found when contemporaneous observations from nearby ionosondes detected echoes from sporadic-E layers. However, when the peak density of these layers was high (foEs> 3 MHz), there were no MSTIDs detected. Similar results are presented using the first station of the LWA, LWA1, to perform all-sky imaging of dense E-region structures (sporadic-E "clouds") via coherent scattering of distant analog TV broadcasts at 55 MHz. These observations were conducted during summer/autumn 2012 and include simultaneous GPS-based observations of F-region disturbances.Left: LWA1 all-sky image of ionospheric echoes of analog TV transmissions at 55.25 MHz. Right: Doppler speed maps for the brightest echoes.

NGC 7538 IRS. 1. Interaction of a Polarized Dust Spiral and a Molecular Outflow

NASA Astrophysics Data System (ADS)

Wright, M. C. H.; Hull, Charles L. H.; Pillai, Thushara; Zhao, Jun-Hui; Sandell, Göran

2014-12-01

We present dust polarization and CO molecular line images of NGC 7538 IRS 1. We combined data from the Submillimeter Array, the Combined Array for Research in Millimeter-wave Astronomy, and the James Clerk Maxwell Telescope to make images with ~2.''5 resolution at 230 and 345 GHz. The images show a remarkable spiral pattern in both the dust polarization and molecular outflow. These data dramatically illustrate the interplay between a high infall rate onto IRS 1 and a powerful outflow disrupting the dense, clumpy medium surrounding the star. The images of the dust polarization and the CO outflow presented here provide observational evidence for the exchange of energy and angular momentum between the infall and the outflow. The spiral dust pattern, which rotates through over 180° from IRS 1, may be a clumpy filament wound up by conservation of angular momentum in the infalling material. The redshifted CO emission ridge traces the dust spiral closely through the MM dust cores, several of which may contain protostars. We propose that the CO maps the boundary layer where the outflow is ablating gas from the dense gas in the spiral.

Shallow subsurface structure estimated from dense aftershock records and microtremor observations in Furukawa district, Miyagi, Japan

NASA Astrophysics Data System (ADS)

Goto, Hiroyuki; Mitsunaga, Hitoshi; Inatani, Masayuki; Iiyama, Kahori; Hada, Koji; Ikeda, Takaaki; Takaya, Toshiyasu; Kimura, Sayaka; Akiyama, Ryohei; Sawada, Sumio; Morikawa, Hitoshi

2017-11-01

We conducted single-site and array observations of microtremors in order to revise the shallow subsurface structure of the Furukawa district, Miyagi, Japan, where severe residential damage was reported during the Great Eastern Japan Earthquake of 2011, off the Pacific coast of Tohoku. The phase velocities of Rayleigh waves are estimated from array observations at three sites, and S-wave velocity models are established. The spatial distribution of predominant periods is estimated for the surface layer, on the basis of the spectral ratio of horizontal and vertical components (H/V) of microtremors obtained from single-site observations. We then compared ground motion records from a dense seismometer network with results of microtremor observations, and revised a model of the shallow (~100 m) subsurface structure in the Furukawa district. The model implies that slower near-surface S-wave velocity and deeper basement are to be found in the southern and eastern areas. It was found that the damage in residential structures was concentrated in an area where the average value for the transfer functions in the frequency range of 2 to 4 Hz was large.

Seismic gradiometry using ambient seismic noise in an anisotropic Earth

NASA Astrophysics Data System (ADS)

de Ridder, S. A. L.; Curtis, A.

2017-05-01

We introduce a wavefield gradiometry technique to estimate both isotropic and anisotropic local medium characteristics from short recordings of seismic signals by inverting a wave equation. The method exploits the information in the spatial gradients of a seismic wavefield that are calculated using dense deployments of seismic arrays. The application of the method uses the surface wave energy in the ambient seismic field. To estimate isotropic and anisotropic medium properties we invert an elliptically anisotropic wave equation. The spatial derivatives of the recorded wavefield are evaluated by calculating finite differences over nearby recordings, which introduces a systematic anisotropic error. A two-step approach corrects this error: finite difference stencils are first calibrated, then the output of the wave-equation inversion is corrected using the linearized impulse response to the inverted velocity anomaly. We test the procedure on ambient seismic noise recorded in a large and dense ocean bottom cable array installed over Ekofisk field. The estimated azimuthal anisotropy forms a circular geometry around the production-induced subsidence bowl. This conforms with results from studies employing controlled sources, and with interferometry correlating long records of seismic noise. Yet in this example, the results were obtained using only a few minutes of ambient seismic noise.

Collective oscillations and coupled modes in confined microfluidic droplet arrays

NASA Astrophysics Data System (ADS)

Schiller, Ulf D.; Fleury, Jean-Baptiste; Seemann, Ralf; Gompper, Gerhard

Microfluidic droplets have a wide range of applications ranging from analytic assays in cellular biology to controlled mixing in chemical engineering. Ensembles of microfluidic droplets are interesting model systems for non-equilibrium many-body phenomena. When flowing in a microchannel, trains of droplets can form microfluidic crystals whose dynamics are governed by long-range hydrodynamic interactions and boundary effects. In this contribution, excitation mechanisms for collective waves in dense and confined microfluidic droplet arrays are investigated by experiments and computer simulations. We demonstrate that distinct modes can be excited by creating specific `defect' patterns in flowing droplet trains. While longitudinal modes exhibit a short-lived cascade of pairs of laterally displacing droplets, transversely excited modes form propagating waves that behave like microfluidic phonons. We show that the confinement induces a coupling between longitudinal and transverse modes. We also investigate the life time of the collective oscillations and discuss possible mechanisms for the onset of instabilities. Our results demonstrate that microfluidic phonons can exhibit effects beyond the linear theory, which can be studied particularly well in dense and confined systems. This work was supported by Deutsche Forschungsgemeinschaft under Grant No. SE 1118/4.

Complexity quantification of dense array EEG using sample entropy analysis.

PubMed

Ramanand, Pravitha; Nampoori, V P N; Sreenivasan, R

2004-09-01

In this paper, a time series complexity analysis of dense array electroencephalogram signals is carried out using the recently introduced Sample Entropy (SampEn) measure. This statistic quantifies the regularity in signals recorded from systems that can vary from the purely deterministic to purely stochastic realm. The present analysis is conducted with an objective of gaining insight into complexity variations related to changing brain dynamics for EEG recorded from the three cases of passive, eyes closed condition, a mental arithmetic task and the same mental task carried out after a physical exertion task. It is observed that the statistic is a robust quantifier of complexity suited for short physiological signals such as the EEG and it points to the specific brain regions that exhibit lowered complexity during the mental task state as compared to a passive, relaxed state. In the case of mental tasks carried out before and after the performance of a physical exercise, the statistic can detect the variations brought in by the intermediate fatigue inducing exercise period. This enhances its utility in detecting subtle changes in the brain state that can find wider scope for applications in EEG based brain studies.

A novel coil array for combined TMS/fMRI experiments at 3 T

PubMed Central

Navarro de Lara, Lucia I.; Windischberger, Christian; Kuehne, Andre; Woletz, Michael; Sieg, Jürgen; Bestmann, Sven; Weiskopf, Nikolaus; Strasser, Bernhard; Moser, Ewald

2014-01-01

Purpose To overcome current limitations in combined transcranial magnetic stimulation (TMS) and functional magnetic resonance imaging (fMRI) studies by employing a dedicated coil array design for 3 Tesla. Methods The state‐of‐the‐art setup for concurrent TMS/fMRI is to use a large birdcage head coil, with the TMS between the subject's head and the MR coil. This setup has drawbacks in sensitivity, positioning, and available imaging techniques. In this study, an ultraslim 7‐channel receive‐only coil array for 3 T, which can be placed between the subject's head and the TMS, is presented. Interactions between the devices are investigated and the performance of the new setup is evaluated in comparison to the state‐of‐the‐art setup. Results MR sensitivity obtained at the depth of the TMS stimulation is increased by a factor of five. Parallel imaging with an acceleration factor of two is feasible with low g‐factors. Possible interactions between TMS and the novel hardware were investigated and were found negligible. Conclusion The novel coil array is safe, strongly improves signal‐to‐noise ratio in concurrent TMS/fMRI experiments, enables parallel imaging, and allows for flexible positioning of the TMS on the head while ensuring efficient TMS stimulation due to its ultraslim design. Magn Reson Med 74:1492–1501, 2015. © 2014 The Authors. Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance in Medicine. PMID:25421603

Spatial and temporal characteristics of V1 microstimulation during chronic implantation of a microelectrode array in a behaving macaque

NASA Astrophysics Data System (ADS)

Davis, T. S.; Parker, R. A.; House, P. A.; Bagley, E.; Wendelken, S.; Normann, R. A.; Greger, B.

2012-12-01

Objective. It has been hypothesized that a vision prosthesis capable of evoking useful visual percepts can be based upon electrically stimulating the primary visual cortex (V1) of a blind human subject via penetrating microelectrode arrays. As a continuation of earlier work, we examined several spatial and temporal characteristics of V1 microstimulation. Approach. An array of 100 penetrating microelectrodes was chronically implanted in V1 of a behaving macaque monkey. Microstimulation thresholds were measured using a two-alternative forced choice detection task. Relative locations of electrically-evoked percepts were measured using a memory saccade-to-target task. Main results. The principal finding was that two years after implantation we were able to evoke behavioural responses to electric stimulation across the spatial extent of the array using groups of contiguous electrodes. Consistent responses to stimulation were evoked at an average threshold current per electrode of 204 ± 49 µA (mean ± std) for groups of four electrodes and 91 ± 25 µA for groups of nine electrodes. Saccades to electrically-evoked percepts using groups of nine electrodes showed that the animal could discriminate spatially distinct percepts with groups having an average separation of 1.6 ± 0.3 mm (mean ± std) in cortex and 1.0° ± 0.2° in visual space. Significance. These results demonstrate chronic perceptual functionality and provide evidence for the feasibility of a cortically-based vision prosthesis for the blind using penetrating microelectrodes.

Embedded binaries and their dense cores

NASA Astrophysics Data System (ADS)

Sadavoy, Sarah I.; Stahler, Steven W.

2017-08-01

We explore the relationship between young, embedded binaries and their parent cores, using observations within the Perseus Molecular Cloud. We combine recently published Very Large Array observations of young stars with core properties obtained from Submillimetre Common-User Bolometer Array 2 observations at 850 μm. Most embedded binary systems are found towards the centres of their parent cores, although several systems have components closer to the core edge. Wide binaries, defined as those systems with physical separations greater than 500 au, show a tendency to be aligned with the long axes of their parent cores, whereas tight binaries show no preferred orientation. We test a number of simple, evolutionary models to account for the observed populations of Class 0 and I sources, both single and binary. In the model that best explains the observations, all stars form initially as wide binaries. These binaries either break up into separate stars or else shrink into tighter orbits. Under the assumption that both stars remain embedded following binary break-up, we find a total star formation rate of 168 Myr-1. Alternatively, one star may be ejected from the dense core due to binary break-up. This latter assumption results in a star formation rate of 247 Myr-1. Both production rates are in satisfactory agreement with current estimates from other studies of Perseus. Future observations should be able to distinguish between these two possibilities. If our model continues to provide a good fit to other star-forming regions, then the mass fraction of dense cores that becomes stars is double what is currently believed.

Electricity from photovoltaic solar cells. Flat-Plate Solar Array Project of the US Department of Energy's National Photovoltaics Program: 10 years of progress

NASA Technical Reports Server (NTRS)

Christensen, Elmer

1985-01-01

The objectives were to develop the flat-plate photovoltaic (PV) array technologies required for large-scale terrestrial use late in the 1980s and in the 1990s; advance crystalline silicon PV technologies; develop the technologies required to convert thin-film PV research results into viable module and array technology; and to stimulate transfer of knowledge of advanced PV materials, solar cells, modules, and arrays to the PV community. Progress reached on attaining these goals, along with future recommendations are discussed.

The Development of a Two-Dimensional Multielectrode Array for Visual Perception Research in the Mammalian Brain.

DTIC Science & Technology

1980-12-01

primary and secondary visual cortex or in the secondary visual cortex itself. When the secondary visual cortex is electrically stimulated , the subject...effect enhances their excitability, which reduces the additional stimulation ( electrical or chemical) required to elicit an action potential. These...and the peripheral area with rods. The rods have a very low light intensity threshold and provide stimulation to optic nerve fibers for low light

Magnetic Fields in the Massive Dense Cores of the DR21 Filament: Weakly Magnetized Cores in a Strongly Magnetized Filament

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ching, Tao-Chung; Lai, Shih-Ping; Zhang, Qizhou

We present Submillimeter Array 880 μ m dust polarization observations of six massive dense cores in the DR21 filament. The dust polarization shows complex magnetic field structures in the massive dense cores with sizes of 0.1 pc, in contrast to the ordered magnetic fields of the parsec-scale filament. The major axes of the massive dense cores appear to be aligned either parallel or perpendicular to the magnetic fields of the filament, indicating that the parsec-scale magnetic fields play an important role in the formation of the massive dense cores. However, the correlation between the major axes of the cores andmore » the magnetic fields of the cores is less significant, suggesting that during the core formation, the magnetic fields below 0.1 pc scales become less important than the magnetic fields above 0.1 pc scales in supporting a core against gravity. Our analysis of the angular dispersion functions of the observed polarization segments yields a plane-of-sky magnetic field strength of 0.4–1.7 mG for the massive dense cores. We estimate the kinematic, magnetic, and gravitational virial parameters of the filament and the cores. The virial parameters show that the gravitational energy in the filament dominates magnetic and kinematic energies, while the kinematic energy dominates in the cores. Our work suggests that although magnetic fields may play an important role in a collapsing filament, the kinematics arising from gravitational collapse must become more important than magnetic fields during the evolution from filaments to massive dense cores.« less

Tracking brain motion during the cardiac cycle using spiral cine-DENSE MRI

PubMed Central

Zhong, Xiaodong; Meyer, Craig H.; Schlesinger, David J.; Sheehan, Jason P.; Epstein, Frederick H.; Larner, James M.; Benedict, Stanley H.; Read, Paul W.; Sheng, Ke; Cai, Jing

2009-01-01

Cardiac-synchronized brain motion is well documented, but the accurate measurement of such motion on the pixel-by-pixel basis has been hampered by the lack of proper imaging technique. In this article, the authors present the implementation of an autotracking spiral cine displacement-encoded stimulation echo (DENSE) magnetic resonance imaging (MRI) technique for the measurement of pulsatile brain motion during the cardiac cycle. Displacement-encoded dynamic MR images of three healthy volunteers were acquired throughout the cardiac cycle using the spiral cine-DENSE pulse sequence gated to the R wave of an electrocardiogram. Pixelwise Lagrangian displacement maps were computed, and 2D displacement as a function of time was determined for selected regions of interests. Different intracranial structures exhibited characteristic motion amplitude, direction, and pattern throughout the cardiac cycle. Time-resolved displacement curves revealed the pathway of pulsatile motion from brain stem to peripheral brain lobes. These preliminary results demonstrated that the spiral cine-DENSE MRI technique can be used to measure cardiac-synchronized pulsatile brain motion on the pixel-by-pixel basis with high temporal∕spatial resolution and sensitivity. PMID:19746774

Rapid prototyping of flexible intrafascicular electrode arrays by picosecond laser structuring.

PubMed

Mueller, Matthias; de la Oliva, Natalia; Del Valle, Jaume; Delgado-Martínez, Ignacio; Navarro, Xavier; Stieglitz, Thomas

2017-12-01

Interfacing the peripheral nervous system can be performed with a large variety of electrode arrays. However, stimulating and recording a nerve while having a reasonable amount of channels limits the number of available systems. Translational research towards human clinical trial requires device safety and biocompatibility but would benefit from design flexibility in the development process to individualize probes. We selected established medical grade implant materials like precious metals and Parylene C to develop a rapid prototyping process for novel intrafascicular electrode arrays using a picosecond laser structuring. A design for a rodent animal model was developed in conjunction with an intrafascicular implantation strategy. Electrode characterization and optimization was performed first in saline solution in vitro before performance and biocompatibility were validated in sciatic nerves of rats in chronic implantation. The novel fabrication process proved to be suitable for prototyping and building intrafascicular electrode arrays. Electrochemical properties of the electrode sites were enhanced and tested for long-term stability. Chronic implantation in the sciatic nerve of rats showed good biocompatibility, selectivity and stable stimulation thresholds. Established medical grade materials can be used for intrafascicular nerve electrode arrays when laser structuring defines structure size in the micro-scale. Design flexibility reduces re-design cycle time and material certificates are beneficial support for safety studies on the way to clinical trials.

In vivo optical modulation of neural signals using monolithically integrated two-dimensional neural probe arrays

PubMed Central

Son, Yoojin; Jenny Lee, Hyunjoo; Kim, Jeongyeon; Shin, Hyogeun; Choi, Nakwon; Justin Lee, C.; Yoon, Eui-Sung; Yoon, Euisik; Wise, Kensall D.; Geun Kim, Tae; Cho, Il-Joo

2015-01-01

Integration of stimulation modalities (e.g. electrical, optical, and chemical) on a large array of neural probes can enable an investigation of important underlying mechanisms of brain disorders that is not possible through neural recordings alone. Furthermore, it is important to achieve this integration of multiple functionalities in a compact structure to utilize a large number of the mouse models. Here we present a successful optical modulation of in vivo neural signals of a transgenic mouse through our compact 2D MEMS neural array (optrodes). Using a novel fabrication method that embeds a lower cladding layer in a silicon substrate, we achieved a thin silicon 2D optrode array that is capable of delivering light to multiple sites using SU-8 as a waveguide core. Without additional modification to the microelectrodes, the measured impedance of the multiple microelectrodes was below 1 MΩ at 1 kHz. In addition, with a low background noise level (±25 μV), neural spikes from different individual neurons were recorded on each microelectrode. Lastly, we successfully used our optrodes to modulate the neural activity of a transgenic mouse through optical stimulation. These results demonstrate the functionality of the 2D optrode array and its potential as a next-generation tool for optogenetic applications. PMID:26494437

A CMOS IC-based multisite measuring system for stimulation and recording in neural preparations in vitro

PubMed Central

Tateno, Takashi; Nishikawa, Jun

2014-01-01

In this report, we describe the system integration of a complementary metal oxide semiconductor (CMOS) integrated circuit (IC) chip, capable of both stimulation and recording of neurons or neural tissues, to investigate electrical signal propagation within cellular networks in vitro. The overall system consisted of three major subunits: a 5.0 × 5.0 mm CMOS IC chip, a reconfigurable logic device (field-programmable gate array, FPGA), and a PC. To test the system, microelectrode arrays (MEAs) were used to extracellularly measure the activity of cultured rat cortical neurons and mouse cortical slices. The MEA had 64 bidirectional (stimulation and recording) electrodes. In addition, the CMOS IC chip was equipped with dedicated analog filters, amplification stages, and a stimulation buffer. Signals from the electrodes were sampled at 15.6 kHz with 16-bit resolution. The measured input-referred circuitry noise was 10.1 μ V root mean square (10 Hz to 100 kHz), which allowed reliable detection of neural signals ranging from several millivolts down to approximately 33 μ Vpp. Experiments were performed involving the stimulation of neurons with several spatiotemporal patterns and the recording of the triggered activity. An advantage over current MEAs, as demonstrated by our experiments, includes the ability to stimulate (voltage stimulation, 5-bit resolution) spatiotemporal patterns in arbitrary subsets of electrodes. Furthermore, the fast stimulation reset mechanism allowed us to record neuronal signals from a stimulating electrode around 3 ms after stimulation. We demonstrate that the system can be directly applied to, for example, auditory neural prostheses in conjunction with an acoustic sensor and a sound processing system. PMID:25346683

A CMOS IC-based multisite measuring system for stimulation and recording in neural preparations in vitro.

PubMed

Tateno, Takashi; Nishikawa, Jun

2014-01-01

In this report, we describe the system integration of a complementary metal oxide semiconductor (CMOS) integrated circuit (IC) chip, capable of both stimulation and recording of neurons or neural tissues, to investigate electrical signal propagation within cellular networks in vitro. The overall system consisted of three major subunits: a 5.0 × 5.0 mm CMOS IC chip, a reconfigurable logic device (field-programmable gate array, FPGA), and a PC. To test the system, microelectrode arrays (MEAs) were used to extracellularly measure the activity of cultured rat cortical neurons and mouse cortical slices. The MEA had 64 bidirectional (stimulation and recording) electrodes. In addition, the CMOS IC chip was equipped with dedicated analog filters, amplification stages, and a stimulation buffer. Signals from the electrodes were sampled at 15.6 kHz with 16-bit resolution. The measured input-referred circuitry noise was 10.1 μ V root mean square (10 Hz to 100 kHz), which allowed reliable detection of neural signals ranging from several millivolts down to approximately 33 μ Vpp. Experiments were performed involving the stimulation of neurons with several spatiotemporal patterns and the recording of the triggered activity. An advantage over current MEAs, as demonstrated by our experiments, includes the ability to stimulate (voltage stimulation, 5-bit resolution) spatiotemporal patterns in arbitrary subsets of electrodes. Furthermore, the fast stimulation reset mechanism allowed us to record neuronal signals from a stimulating electrode around 3 ms after stimulation. We demonstrate that the system can be directly applied to, for example, auditory neural prostheses in conjunction with an acoustic sensor and a sound processing system.

1024-Pixel CMOS Multimodality Joint Cellular Sensor/Stimulator Array for Real-Time Holistic Cellular Characterization and Cell-Based Drug Screening.

PubMed

Park, Jong Seok; Aziz, Moez Karim; Li, Sensen; Chi, Taiyun; Grijalva, Sandra Ivonne; Sung, Jung Hoon; Cho, Hee Cheol; Wang, Hua

2018-02-01

This paper presents a fully integrated CMOS multimodality joint sensor/stimulator array with 1024 pixels for real-time holistic cellular characterization and drug screening. The proposed system consists of four pixel groups and four parallel signal-conditioning blocks. Every pixel group contains 16 × 16 pixels, and each pixel includes one gold-plated electrode, four photodiodes, and in-pixel circuits, within a pixel footprint. Each pixel supports real-time extracellular potential recording, optical detection, charge-balanced biphasic current stimulation, and cellular impedance measurement for the same cellular sample. The proposed system is fabricated in a standard 130-nm CMOS process. Rat cardiomyocytes are successfully cultured on-chip. Measured high-resolution optical opacity images, extracellular potential recordings, biphasic current stimulations, and cellular impedance images demonstrate the unique advantages of the system for holistic cell characterization and drug screening. Furthermore, this paper demonstrates the use of optical detection on the on-chip cultured cardiomyocytes to real-time track their cyclic beating pattern and beating rate.

Adaptive enhancement of learning protocol in hippocampal cultured networks grown on multielectrode arrays

PubMed Central

Pimashkin, Alexey; Gladkov, Arseniy; Mukhina, Irina; Kazantsev, Victor

2013-01-01

Learning in neuronal networks can be investigated using dissociated cultures on multielectrode arrays supplied with appropriate closed-loop stimulation. It was shown in previous studies that weakly respondent neurons on the electrodes can be trained to increase their evoked spiking rate within a predefined time window after the stimulus. Such neurons can be associated with weak synaptic connections in nearby culture network. The stimulation leads to the increase in the connectivity and in the response. However, it was not possible to perform the learning protocol for the neurons on electrodes with relatively strong synaptic inputs and responding at higher rates. We proposed an adaptive closed-loop stimulation protocol capable to achieve learning even for the highly respondent electrodes. It means that the culture network can reorganize appropriately its synaptic connectivity to generate a desired response. We introduced an adaptive reinforcement condition accounting for the response variability in control stimulation. It significantly enhanced the learning protocol to a large number of responding electrodes independently on its base response level. We also found that learning effect preserved after 4–6 h after training. PMID:23745105

Application of wavefield compressive sensing in surface wave tomography

NASA Astrophysics Data System (ADS)

Zhan, Zhongwen; Li, Qingyang; Huang, Jianping

2018-06-01

Dense arrays allow sampling of seismic wavefield without significant aliasing, and surface wave tomography has benefitted from exploiting wavefield coherence among neighbouring stations. However, explicit or implicit assumptions about wavefield, irregular station spacing and noise still limit the applicability and resolution of current surface wave methods. Here, we propose to apply the theory of compressive sensing (CS) to seek a sparse representation of the surface wavefield using a plane-wave basis. Then we reconstruct the continuous surface wavefield on a dense regular grid before applying any tomographic methods. Synthetic tests demonstrate that wavefield CS improves robustness and resolution of Helmholtz tomography and wavefield gradiometry, especially when traditional approaches have difficulties due to sub-Nyquist sampling or complexities in wavefield.

Lithographically Defined, Room Temperature Low Threshold Subwavelength Red-Emitting Hybrid Plasmonic Lasers.

PubMed

Liu, Ning; Gocalinska, Agnieszka; Justice, John; Gity, Farzan; Povey, Ian; McCarthy, Brendan; Pemble, Martyn; Pelucchi, Emanuele; Wei, Hong; Silien, Christophe; Xu, Hongxing; Corbett, Brian

2016-12-14

Hybrid plasmonic lasers provide deep subwavelength optical confinement, strongly enhanced light-matter interaction and together with nanoscale footprint promise new applications in optical communication, biosensing, and photolithography. The subwavelength hybrid plasmonic lasers reported so far often use bottom-up grown nanowires, nanorods, and nanosquares, making it difficult to integrate these devices into industry-relevant high density plasmonic circuits. Here, we report the first experimental demonstration of AlGaInP based, red-emitting hybrid plasmonic lasers at room temperature using lithography based fabrication processes. Resonant cavities with deep subwavelength 2D and 3D mode confinement of λ 2 /56 and λ 3 /199, respectively, are demonstrated. A range of cavity geometries (waveguides, rings, squares, and disks) show very low lasing thresholds of 0.6-1.8 mJ/cm 2 with wide gain bandwidth (610 nm-685 nm), which are attributed to the heterogeneous geometry of the gain material, the optimized etching technique, and the strong overlap of the gain material with the plasmonic modes. Most importantly, we establish the connection between mode confinements and enhanced absorption and stimulated emission, which plays critical roles in maintaining low lasing thresholds at extremely small hybrid plasmonic cavities. Our results pave the way for the further integration of dense arrays of hybrid plasmonic lasers with optical and electronic technology platforms.

The New NASA-STD-4005 and NASA-HDBK-4006, Essentials for Direct-Drive Solar Electric Propulsion

NASA Technical Reports Server (NTRS)

Ferguson, Dale C.

2007-01-01

High voltage solar arrays are necessary for direct-drive solar electric propulsion, which has many advantages, including simplicity and high efficiency. Even when direct-drive is not used, the use of high voltage solar arrays leads to power transmission and conversion efficiencies in electric propulsion Power Management and Distribution. Nevertheless, high voltage solar arrays may lead to temporary power disruptions, through the so-called primary electrostatic discharges, and may permanently damage arrays, through the so-called permanent sustained discharges between array strings. Design guidance is needed to prevent these solar array discharges, and to prevent high power drains through coupling between the electric propulsion devices and the high voltage solar arrays. While most electric propulsion systems may operate outside of Low Earth Orbit, the plasmas produced by their thrusters may interact with the high voltage solar arrays in many ways similarly to Low Earth Orbit plasmas. A brief description of previous experiences with high voltage electric propulsion systems will be given in this paper. There are two new official NASA documents available free through the NASA Standards website to help in designing and testing high voltage solar arrays for electric propulsion. They are NASA-STD-4005, the Low Earth Orbit Spacecraft Charging Design Standard, and NASA-HDBK-4006, the Low Earth Orbit Spacecraft Charging Design Handbook. Taken together, they can both educate the high voltage array designer in the engineering and science of spacecraft charging in the presence of dense plasmas and provide techniques for designing and testing high voltage solar arrays to prevent electrical discharges and power drains.

Stimulated Electronic X-Ray Raman Scattering

NASA Astrophysics Data System (ADS)

Weninger, Clemens; Purvis, Michael; Ryan, Duncan; London, Richard A.; Bozek, John D.; Bostedt, Christoph; Graf, Alexander; Brown, Gregory; Rocca, Jorge J.; Rohringer, Nina

2013-12-01

We demonstrate strong stimulated inelastic x-ray scattering by resonantly exciting a dense gas target of neon with femtosecond, high-intensity x-ray pulses from an x-ray free-electron laser (XFEL). A small number of lower energy XFEL seed photons drive an avalanche of stimulated resonant inelastic x-ray scattering processes that amplify the Raman scattering signal by several orders of magnitude until it reaches saturation. Despite the large overall spectral width, the internal spiky structure of the XFEL spectrum determines the energy resolution of the scattering process in a statistical sense. This is demonstrated by observing a stochastic line shift of the inelastically scattered x-ray radiation. In conjunction with statistical methods, XFELs can be used for stimulated resonant inelastic x-ray scattering, with spectral resolution smaller than the natural width of the core-excited, intermediate state.

Transparent, conformable, active multielectrode array using organic electrochemical transistors.

PubMed

Lee, Wonryung; Kim, Dongmin; Matsuhisa, Naoji; Nagase, Masae; Sekino, Masaki; Malliaras, George G; Yokota, Tomoyuki; Someya, Takao

2017-10-03

Mechanically flexible active multielectrode arrays (MEA) have been developed for local signal amplification and high spatial resolution. However, their opaqueness limited optical observation and light stimulation during use. Here, we show a transparent, ultraflexible, and active MEA, which consists of transparent organic electrochemical transistors (OECTs) and transparent Au grid wirings. The transparent OECT is made of Au grid electrodes and has shown comparable performance with OECTs with nontransparent electrodes/wirings. The transparent active MEA realizes the spatial mapping of electrocorticogram electrical signals from an optogenetic rat with 1-mm spacing and shows lower light artifacts than noise level. Our active MEA would open up the possibility of precise investigation of a neural network system with direct light stimulation.

Qualities of Single Electrode Stimulation as a Function of Rate and Place of Stimulation with a Cochlear Implant

PubMed Central

Landsberger, David M.; Vermeire, Katrien; Claes, Annes; Van Rompaey, Vincent; Van de Heyning, Paul

2015-01-01

Objectives Although it has been previously shown that changes in temporal coding produce changes in pitch in all cochlear regions, research has suggested that temporal coding might be best encoded in relatively apical locations. We hypothesized that although temporal coding may provide useable information at any cochlear location, low rates of stimulation might provide better sound quality in apical regions that are more likely to encode temporal information in the normal ear. In the present study, sound qualities of single electrode pulse trains were scaled to provide insight into the combined effects of cochlear location and stimulation rate on sound quality. Design Ten long term users of MED-EL cochlear implants with 31 mm electrode arrays (Standard or FLEXSOFT) were asked to scale the sound quality of single electrode pulse trains in terms of how “Clean”, “Noisy”, “High”, and “Annoying” they sounded. Pulse trains were presented on most electrodes between 1 and 12 representing the entire range of the long electrode array at stimulation rates of 100, 150, 200, 400, or 1500 pulses per second. Results While high rates of stimulation are scaled as having a “Clean” sound quality across the entire array, only the most apical electrodes (typically 1 through 3) were considered “Clean” at low rates. Low rates on electrodes 6 through 12 were not rated as “Clean” while the low rate quality of electrodes 4 and 5 were typically in between. Scaling of “Noisy” responses provided an approximately inverse pattern as “Clean” responses. “High” responses show the trade-off between rate and place of stimulation on pitch. Because “High” responses did not correlate with “Clean” responses, subjects were not rating sound quality based on pitch. Conclusions If explicit temporal coding is to be provided in a cochlear implant, it is likely to sound better when provided apically. Additionally, the finding that low rates sound clean only at apical places of stimulation is consistent with previous findings that a change in rate of stimulation corresponds to an equivalent change in perceived pitch at apical locations. Collectively, the data strongly suggests that temporal coding with a cochlear implant is optimally provided by electrodes placed well into the second cochlear turn. PMID:26583480

A pilot controlled trial of a combination of dense cranial electroacupuncture stimulation and body acupuncture for post-stroke depression.

PubMed

Man, Sui-Cheung; Hung, Ben H B; Ng, Roger M K; Yu, Xiao-Chun; Cheung, Hobby; Fung, Mandy P M; Li, Leonard S W; Leung, Kwok-Pui; Leung, Kei-Pui; Tsang, Kevin W Y; Ziea, Eric; Wong, Vivian T; Zhang, Zhang-Jin

2014-07-19

Our previous studies have demonstrated the treatment benefits of dense cranial electroacupuncture stimulation (DCEAS), a novel brain stimulation therapy in patients with major depression, postpartum depression and obsessive-compulsive disorder. The purpose of the present study was to further evaluate the effectiveness of DCEAS combined with body acupuncture and selective serotonin reuptake inhibitors (SSRIs) in patients with post-stroke depression (PSD). In a single-blind, randomized controlled trial, 43 patients with PSD were randomly assigned to 12 sessions of DCEAS plus SSRI plus body electroacupuncture (n = 23), or sham (non-invasive cranial electroacupuncture, n-CEA) plus SSRI plus body electroacupuncture (n = 20) for 3 sessions per week over 4 weeks. Treatment outcomes were measured using the 17-item Hamilton Depression Rating Scale (HAMD-17), the Clinical Global Impression - Severity scale (CGI-S) and Barthel Index (BI), a measure used to evaluate movement ability associated with daily self-caring activity. DCEAS produced a significantly greater reduction of both HAMD-17 and CGI-S as early as week 1 and CGI-S at endpoint compared to n-CEA, but subjects of n-CEA group exhibited a significantly greater improvement on BI at week 4 than DCEAS. Incidence of adverse events was not different in the two groups. These results indicate that DCEAS could be effective in reducing stroke patients' depressive symptoms. Superficial electrical stimulation in n-CEA group may be beneficial in improving movement disability of stroke patients. A combination of DCEAS and body acupuncture can be considered a treatment option for neuropsychiatric sequelae of stroke. http://www.clinicaltrials.gov, NCT01174394.

A novel flexible cuff-like microelectrode for dual purpose, acute and chronic electrical interfacing with the mouse cervical vagus nerve

NASA Astrophysics Data System (ADS)

Caravaca, A. S.; Tsaava, T.; Goldman, L.; Silverman, H.; Riggott, G.; Chavan, S. S.; Bouton, C.; Tracey, K. J.; Desimone, R.; Boyden, E. S.; Sohal, H. S.; Olofsson, P. S.

2017-12-01

Objective. Neural reflexes regulate immune responses and homeostasis. Advances in bioelectronic medicine indicate that electrical stimulation of the vagus nerve can be used to treat inflammatory disease, yet the understanding of neural signals that regulate inflammation is incomplete. Current interfaces with the vagus nerve do not permit effective chronic stimulation or recording in mouse models, which is vital to studying the molecular and neurophysiological mechanisms that control inflammation homeostasis in health and disease. We developed an implantable, dual purpose, multi-channel, flexible ‘microelectrode’ array, for recording and stimulation of the mouse vagus nerve. Approach. The array was microfabricated on an 8 µm layer of highly biocompatible parylene configured with 16 sites. The microelectrode was evaluated by studying the recording and stimulation performance. Mice were chronically implanted with devices for up to 12 weeks. Main results. Using the microelectrode in vivo, high fidelity signals were recorded during physiological challenges (e.g potassium chloride and interleukin-1β), and electrical stimulation of the vagus nerve produced the expected significant reduction of blood levels of tumor necrosis factor (TNF) in endotoxemia. Inflammatory cell infiltration at the microelectrode 12 weeks of implantation was limited according to radial distribution analysis of inflammatory cells. Significance. This novel device provides an important step towards a viable chronic interface for cervical vagus nerve stimulation and recording in mice.

Spread of cochlear excitation during stimulation with pulsed infrared radiation: inferior colliculus measurements

NASA Astrophysics Data System (ADS)

Richter, C.-P.; Rajguru, S. M.; Matic, A. I.; Moreno, E. L.; Fishman, A. J.; Robinson, A. M.; Suh, E.; Walsh, J. T., Jr.

2011-10-01

Infrared neural stimulation (INS) has received considerable attention over the last few years. It provides an alternative method to artificially stimulate neurons without electrical current or the introduction of exogenous chromophores. One of the primary benefits of INS could be the improved spatial selectivity when compared with electrical stimulation. In the present study, we have evaluated the spatial selectivity of INS in the acutely damaged cochlea of guinea pigs and compared it to stimulation with acoustic tone pips in normal-hearing animals. The radiation was delivered via a 200 µm diameter optical fiber, which was inserted through a cochleostomy into the scala tympani of the basal cochlear turn. The stimulated section along the cochlear spiral ganglion was estimated from the neural responses recorded from the central nucleus of the inferior colliculus (ICC). ICC responses were recorded in response to cochlear INS using a multichannel penetrating electrode array. Spatial tuning curves (STCs) were constructed from the responses. For INS, approximately 55% of the activation profiles showed a single maximum, ~22% had two maxima and ~13% had multiple maxima. The remaining 10% of the profiles occurred at the limits of the electrode array and could not be classified. The majority of ICC STCs indicated that the spread of activation evoked by optical stimuli is comparable to that produced by acoustic tone pips.

Single-Source Gravitational Wave Limits From the J1713+0747 24-hr Global Campaign

NASA Astrophysics Data System (ADS)

Dolch, T.; NANOGrav Collaboration; Ellis, J. A.; Chatterjee, S.; Cordes, J. M.; Lam, M. T.; Bassa, C.; Bhattacharyya, B.; Champion, D. J.; Cognard, I.; Crowter, K.; Demorest, P. B.; Hessels, J. W. T.; Janssen, G.; Jenet, F. A.; Jones, G.; Jordan, C.; Karuppusamy, R.; Keith, M.; Kondratiev, V. I.; Kramer, M.; Lazarus, P.; Lazio, T. J. W.; Lorimer, D. R.; Madison, D. R.; McLaughlin, M. A.; Palliyaguru, N.; Perrodin, D.; Ransom, S. M.; Roy, J.; Shannon, R. M.; Smits, R.; Stairs, I. H.; Stappers, B. W.; Stinebring, D. R.; Stovall, K.; Verbiest, J. P. W.; Zhu, W. W.

2016-05-01

Dense, continuous pulsar timing observations over a 24-hr period provide a method for probing intermediate gravitational wave (GW) frequencies from 10 microhertz to 20 millihertz. The European Pulsar Timing Array (EPTA), the North American Nanohertz Observatory for Gravitational Waves (NANOGrav), the Parkes Pulsar Timing Array (PPTA), and the combined International Pulsar Timing Array (IPTA) all use millisecond pulsar observations to detect or constrain GWs typically at nanohertz frequencies. In the case of the IPTA's nine-telescope 24-Hour Global Campaign on millisecond pulsar J1713+0747, GW limits in the intermediate frequency regime can be produced. The negligible change in dispersion measure during the observation minimizes red noise in the timing residuals, constraining any contributions from GWs due to individual sources. At 10-5 Hz, the 95% upper limit on strain is 10-11 for GW sources in the pulsar's direction.

Sub-GeV dark matter detection with electron recoils in carbon nanotubes

NASA Astrophysics Data System (ADS)

Cavoto, G.; Luchetta, F.; Polosa, A. D.

2018-01-01

Directional detection of Dark Matter particles (DM) in the MeV mass range could be accomplished by studying electron recoils in large arrays of parallel carbon nanotubes. In a scattering process with a lattice electron, a DM particle might transfer sufficient energy to eject it from the nanotube surface. An external electric field is added to drive the electron from the open ends of the array to the detection region. The anisotropic response of this detection scheme, as a function of the orientation of the target with respect to the DM wind, is calculated, and it is concluded that no direct measurement of the electron ejection angle is needed to explore significant regions of the light DM exclusion plot. A compact sensor, in which the cathode element is substituted with a dense array of parallel carbon nanotubes, could serve as the basic detection unit.

Observations and interpretation of fundamental mode Rayleigh wavefields recorded by the Transportable Array (USArray)

USGS Publications Warehouse

Pollitz, F.F.

2008-01-01

Broadband recordings of the dense Transportable Array (TA) in the western United States provide unparalleled detailed images of long-period seismic surface wavefields. With 400 stations spanning most of the western United States, wavefronts of fundamental mode Rayleigh waves may be visualized coherently across the array at periods ???40 s. In order to constrain the Rayleigh wave phase velocity structure in the western United States, I assemble a data set of vertical component seismograms from 53 teleseismic events recorded by the TA from April 2006 to October 2007. Complex amplitude spectra from these recordings at peni ods 27-100 s are interpreted using the multiplane wave tomographic method of Friederich and Wielandt (1995) and Pollitz (1999). This analysis yields detailed surface wave phase velocity and three-dimensional shear wave velocity patterns across the North American plate boundary zone, elucidating the active processes in the highly heterogeneous western U.S. upper mantle.

Nonlinear site response in medium magnitude earthquakes near Parkfield, California

USGS Publications Warehouse

Rubinstein, Justin L.

2011-01-01

Careful analysis of strong-motion recordings of 13 medium magnitude earthquakes (3.7 ≤ M ≤ 6.5) in the Parkfield, California, area shows that very modest levels of shaking (approximately 3.5% of the acceleration of gravity) can produce observable changes in site response. Specifically, I observe a drop and subsequent recovery of the resonant frequency at sites that are part of the USGS Parkfield dense seismograph array (UPSAR) and Turkey Flat array. While further work is necessary to fully eliminate other models, given that these frequency shifts correlate with the strength of shaking at the Turkey Flat array and only appear for the strongest shaking levels at UPSAR, the most plausible explanation for them is that they are a result of nonlinear site response. Assuming this to be true, the observation of nonlinear site response in small (M M 6.5 San Simeon earthquake and the 2004 M 6 Parkfield earthquake).

A Clash of Bottom-Up and Top-Down Processes in Visual Search: The Reversed Letter Effect Revisited

ERIC Educational Resources Information Center

Zhaoping, Li; Frith, Uta

2011-01-01

It is harder to find the letter "N" among its mirror reversals than vice versa, an inconvenient finding for bottom-up saliency accounts based on primary visual cortex (V1) mechanisms. However, in line with this account, we found that in dense search arrays, gaze first landed on either target equally fast. Remarkably, after first landing,…

How Toddlers and Preschoolers Learn to Uniquely Identify Referents for Others: A Training Study

ERIC Educational Resources Information Center

Matthews, Danielle; Lieven, Elena; Tomasello, Michael

2007-01-01

This training study investigates how children learn to refer to things unambiguously. Two hundred twenty-four children aged 2.6, 3.6, and 4.6 years were pre- and post-tested for their ability to request stickers from a dense array. Between test sessions, children were assigned to a training condition in which they (a) asked for stickers from an…

A COMPARISON OF AIRFLOW PATTERNS FROM THE QUIC MODEL AND AN ATMOSPHERIC WIND TUNNEL FOR A TWO-DIMENSIONAL BUILDING ARRAY AND A MULTI-CITY BLOCK REGION NEAR THE WORLD TRADE CENTER SITE

EPA Science Inventory

Dispersion of pollutants in densely populated urban areas is a research area of clear importance. Currently, few numerical tools exist capable of describing airflow and dispersion patterns in these complex regions in a time efficient manner. (QUIC), Quick Urban & Industrial C...

Plastic fiber scintillator response to fast neutrons

NASA Astrophysics Data System (ADS)

Danly, C. R.; Sjue, S.; Wilde, C. H.; Merrill, F. E.; Haight, R. C.

2014-11-01

The Neutron Imaging System at NIF uses an array of plastic scintillator fibers in conjunction with a time-gated imaging system to form an image of the neutron emission from the imploded capsule. By gating on neutrons that have scattered from the 14.1 MeV DT energy to lower energy ranges, an image of the dense, cold fuel around the hotspot is also obtained. An unmoderated spallation neutron beamline at the Weapons Neutron Research facility at Los Alamos was used in conjunction with a time-gated imaging system to measure the yield of a scintillating fiber array over several energy bands ranging from 1 to 15 MeV. The results and comparison to simulation are presented.

Plastic fiber scintillator response to fast neutrons.

PubMed

Danly, C R; Sjue, S; Wilde, C H; Merrill, F E; Haight, R C

2014-11-01

The Neutron Imaging System at NIF uses an array of plastic scintillator fibers in conjunction with a time-gated imaging system to form an image of the neutron emission from the imploded capsule. By gating on neutrons that have scattered from the 14.1 MeV DT energy to lower energy ranges, an image of the dense, cold fuel around the hotspot is also obtained. An unmoderated spallation neutron beamline at the Weapons Neutron Research facility at Los Alamos was used in conjunction with a time-gated imaging system to measure the yield of a scintillating fiber array over several energy bands ranging from 1 to 15 MeV. The results and comparison to simulation are presented.

Nanostructured cavity devices for extracellular stimulation of HL-1 cells.

PubMed

Czeschik, Anna; Rinklin, Philipp; Derra, Ulrike; Ullmann, Sabrina; Holik, Peter; Steltenkamp, Siegfried; Offenhäusser, Andreas; Wolfrum, Bernhard

2015-01-01

Microelectrode arrays (MEAs) are state-of-the-art devices for extracellular recording and stimulation on biological tissue. Furthermore, they are a relevant tool for the development of biomedical applications like retina, cochlear and motor prostheses, cardiac pacemakers and drug screening. Hence, research on functional cell-sensor interfaces, as well as the development of new surface structures and modifications for improved electrode characteristics, is a vivid and well established field. However, combining single-cell resolution with sufficient signal coupling remains challenging due to poor cell-electrode sealing. Furthermore, electrodes with diameters below 20 µm often suffer from a high electrical impedance affecting the noise during voltage recordings. In this study, we report on a nanocavity sensor array for voltage-controlled stimulation and extracellular action potential recordings on cellular networks. Nanocavity devices combine the advantages of low-impedance electrodes with small cell-chip interfaces, preserving a high spatial resolution for recording and stimulation. A reservoir between opening aperture and electrode is provided, allowing the cell to access the structure for a tight cell-sensor sealing. We present the well-controlled fabrication process and the effect of cavity formation and electrode patterning on the sensor's impedance. Further, we demonstrate reliable voltage-controlled stimulation using nanostructured cavity devices by capturing the pacemaker of an HL-1 cell network.

Analyte species and concentration identification using differentially functionalized microcantilever arrays and artificial neural networks

DOE Office of Scientific and Technical Information (OSTI.GOV)

Senesac, Larry R; Datskos, Panos G; Sepaniak, Michael J

2006-01-01

In the present work, we have performed analyte species and concentration identification using an array of ten differentially functionalized microcantilevers coupled with a back-propagation artificial neural network pattern recognition algorithm. The array consists of ten nanostructured silicon microcantilevers functionalized by polymeric and gas chromatography phases and macrocyclic receptors as spatially dense, differentially responding sensing layers for identification and quantitation of individual analyte(s) and their binary mixtures. The array response (i.e. cantilever bending) to analyte vapor was measured by an optical readout scheme and the responses were recorded for a selection of individual analytes as well as several binary mixtures. Anmore » artificial neural network (ANN) was designed and trained to recognize not only the individual analytes and binary mixtures, but also to determine the concentration of individual components in a mixture. To the best of our knowledge, ANNs have not been applied to microcantilever array responses previously to determine concentrations of individual analytes. The trained ANN correctly identified the eleven test analyte(s) as individual components, most with probabilities greater than 97%, whereas it did not misidentify an unknown (untrained) analyte. Demonstrated unique aspects of this work include an ability to measure binary mixtures and provide both qualitative (identification) and quantitative (concentration) information with array-ANN-based sensor methodologies.« less

Ontogenetic changes in the olfactory antennules of the shore crab, Hemigrapsus oregonensis, maintain sniffing function during growth

PubMed Central

Waldrop, Lindsay D.; Hann, Miranda; Henry, Amy K.; Kim, Agnes; Punjabi, Ayesha; Koehl, M. A. R.

2015-01-01

Malacostracan crustaceans capture odours using arrays of chemosensory hairs (aesthetascs) on antennules. Lobsters and stomatopods have sparse aesthetascs on long antennules that flick with a rapid downstroke when water flows between the aesthetascs and a slow return stroke when water is trapped within the array (sniffing). Changes in velocity only cause big differences in flow through an array in a critical range of hair size, spacing and speed. Crabs have short antennules bearing dense arrays of flexible aesthetascs that splay apart during downstroke and clump together during return. Can crabs sniff, and when during ontogeny are they big enough to sniff? Antennules of Hemigrapsus oregonensis representing an ontogenetic series from small juveniles to adults were used to design dynamically scaled physical models. Particle image velocimetry quantified fluid flow through each array and showed that even very small crabs capture a new water sample in their arrays during the downstroke and retain that sample during return stroke. Comparison with isometrically scaled antennules suggests that reduction in aesthetasc flexural stiffness during ontogeny, in addition to increase in aesthetasc number and decrease in relative size, maintain sniffing as crabs grow. Sniffing performance of intermediate-sized juveniles was worse than for smaller and larger crabs. PMID:25411408

Ontogenetic changes in the olfactory antennules of the shore crab, Hemigrapsus oregonensis, maintain sniffing function during growth.

PubMed

Waldrop, Lindsay D; Hann, Miranda; Henry, Amy K; Kim, Agnes; Punjabi, Ayesha; Koehl, M A R

2015-01-06

Malacostracan crustaceans capture odours using arrays of chemosensory hairs (aesthetascs) on antennules. Lobsters and stomatopods have sparse aesthetascs on long antennules that flick with a rapid downstroke when water flows between the aesthetascs and a slow return stroke when water is trapped within the array (sniffing). Changes in velocity only cause big differences in flow through an array in a critical range of hair size, spacing and speed. Crabs have short antennules bearing dense arrays of flexible aesthetascs that splay apart during downstroke and clump together during return. Can crabs sniff, and when during ontogeny are they big enough to sniff? Antennules of Hemigrapsus oregonensis representing an ontogenetic series from small juveniles to adults were used to design dynamically scaled physical models. Particle image velocimetry quantified fluid flow through each array and showed that even very small crabs capture a new water sample in their arrays during the downstroke and retain that sample during return stroke. Comparison with isometrically scaled antennules suggests that reduction in aesthetasc flexural stiffness during ontogeny, in addition to increase in aesthetasc number and decrease in relative size, maintain sniffing as crabs grow. Sniffing performance of intermediate-sized juveniles was worse than for smaller and larger crabs. © 2014 The Author(s) Published by the Royal Society. All rights reserved.

Feasibility of microelectrode array (MEA) based on silicone-polyimide hybrid for retina prosthesis.

PubMed

Kim, Eui Tae; Kim, Cinoo; Lee, Seung Woo; Seo, Jong-Mo; Chung, Hum; Kim, Sung June

2009-09-01

To adopt micropatterning technology in manufacturing silicone elastomer-based microelectrode arrays for retinal stimulation, a silicone-polyimide hybrid microelectrode array was proposed and tested in vivo. Gold microelectrodes were created by semiconductor manufacturing technology based on polyimide and were hybridized with silicone elastomer by spin coating. The stability of the hybrid between the two materials was flex and blister tested. The feasibility of the hybrid electrode was evaluated in the rabbit eye by reviewing optical coherence tomography (OCT) findings after suprachoroidal implantation. The flex test showed no dehiscence between the two materials for 24 hours of alternative flexion and extension from -45.0 degrees to +45.0 degrees . During the blister test, delamination was observed at 8.33 +/- 1.36 psi of pressure stress; however, this property was improved to 11.50 +/- 1.04 psi by oxygen plasma treatment before hybridization. OCT examination revealed that the implanted electrodes were safely located in the suprachoroidal space during the 4-week follow-up period. The silicone-polyimide hybrid microelectrode array showed moderate physical properties, which are suitable for in vivo application. Appropriate pretreatment before hybridization improved electrode stability. In vivo testing indicated that this electrode is suitable as a stimulation electrode in artificial retina.

GaN-based micro-LED arrays on flexible substrates for optical cochlear implants

NASA Astrophysics Data System (ADS)

Goßler, Christian; Bierbrauer, Colin; Moser, Rüdiger; Kunzer, Michael; Holc, Katarzyna; Pletschen, Wilfried; Köhler, Klaus; Wagner, Joachim; Schwaerzle, Michael; Ruther, Patrick; Paul, Oliver; Neef, Jakob; Keppeler, Daniel; Hoch, Gerhard; Moser, Tobias; Schwarz, Ulrich T.

2014-05-01

Currently available cochlear implants are based on electrical stimulation of the spiral ganglion neurons. Optical stimulation with arrays of micro-sized light-emitting diodes (µLEDs) promises to increase the number of distinguishable frequencies. Here, the development of a flexible GaN-based micro-LED array as an optical cochlear implant is reported for application in a mouse model. The fabrication of 15 µm thin and highly flexible devices is enabled by a laser-based layer transfer process of the GaN-LEDs from sapphire to a polyimide-on-silicon carrier wafer. The fabricated 50 × 50 µm2 LEDs are contacted via conducting paths on both p- and n-sides of the LEDs. Up to three separate channels could be addressed. The probes, composed of a linear array of the said µLEDs bonded to the flexible polyimide substrate, are peeled off the carrier wafer and attached to flexible printed circuit boards. Probes with four µLEDs and a width of 230 µm are successfully implanted in the mouse cochlea both in vitro and in vivo. The LEDs emit 60 µW at 1 mA after peel-off, corresponding to a radiant emittance of 6 mW mm-2.

Identical Aftershocks from the Main Rupture Zone 10 Months After the Mw=7.6 September 5, 2012, Nicoya, Costa Rica, Earthquake

NASA Astrophysics Data System (ADS)

Protti, M.; Alfaro-Diaz, R.; Brenn, G. R.; Fasola, S.; Murillo, A.; Marshall, J. S.; Gardner, T. W.

2013-12-01

Over a two weeks period and as part of a Keck Geology Consortium summer research project, we installed a dense broad band seismic array directly over the rupture zone of the Nicoya, September 5th, 2012, Mw=7.6 earthquake. The network consisted of 5 Trillium compact seismometers and Taurus digitizers from Nanometrics, defining a triangular area of ~20 km per side. Also located within this area are 3 stations of the Nicoya permanent broadband network. One side of the triangular area, along the west coast of the Nicoya peninsula, is parallel to the trench and the apex lies 15 km landward. The plate interface and rupture zone of the Nicoya 2012 earthquake are located 16 km below the trench-parallel side and 25 km below the apex of this triangular footprint. Station spacing ranged from 3 to 14 km. This dense array operated from July 2nd to July 17th, 2013. On June 23rd, eight days before we installed this array, an Mw=5.4 aftershock (one of the only 5 aftershocks of the Nicoya Mw=7.6 earthquake with magnitudes above 5.0) occurred directly beneath the area of our temporary network. Preliminary analysis of the data shows that we recorded several identical aftershocks with magnitudes below 1.0 that locate some 18 km below our network. We will present detailed locations of these small aftershocks and their relationship with the June 23rd, 2013 aftershock and the September 5th, 2012, mainshock.

New constraints on micro-seismicity and stress state in the western part of the North Anatolian Fault Zone: Observations from a dense seismic array

NASA Astrophysics Data System (ADS)

Altuncu Poyraz, Selda; Teoman, M. Uğur; Türkelli, Niyazi; Kahraman, Metin; Cambaz, Didem; Mutlu, Ahu; Rost, Sebastian; Houseman, Gregory A.; Thompson, David A.; Cornwell, David; Utkucu, Murat; Gülen, Levent

2015-08-01

With the aim of extensively investigating the crustal structure beneath the western segment of the North Anatolian Fault Zone where it splays into northern and southern branches, a temporary seismic network (dense array for North Anatolia-DANA) consisting of 70 stations was deployed in early May 2012 and operated for 18 months in the Sakarya region during the FaultLab experiment. Out of 2437 events contaminated by explosions, we extracted 1371 well located earthquakes. The enhanced station coverage having a nominal station spacing of 7 km, lead to a minimum magnitude calculation of 0.1. Horizontal and vertical location uncertainties within the array do not exceed 0.8 km and 0.9 km, respectively. We observe considerable seismic activity along both branches of the fault where the depth of the seismogenic zone was mostly confined to 15 km. Using our current earthquake catalog we obtained a b-value of 1. We also mapped the b-value variation with depth and observed a gradual decrease. Furthermore, we determined the source parameters of 41 earthquakes with magnitudes greater than 1.8 using P-wave first motion polarity method. Regional Moment Tensor Inversion method was also applied to earthquakes with magnitudes greater than 3.0. Focal mechanism solutions confirm that Sakarya and its vicinity is stressed by a compressional regime showing a primarily oblique-slip motion character. Stress tensor analysis indicates that the maximum principal stress is aligned in WNW-ESE direction and the tensional axis is aligned in NNE-SSW direction.

Complete disintegration of the microtubular cytoskeleton precedes its auxin-mediated reconstruction in postmitotic maize root cells

NASA Technical Reports Server (NTRS)

Baluska, F.; Barlow, P. W.; Volkmann, D.

1996-01-01

The inhibitory action of 0.1 microM auxin (IAA) on maize root growth was closely associated with a rapid and complete disintegration of the microtubular (MT) cytoskeleton, as visualized by indirect immunofluorescence of tubulin, throughout the growth region. After 30 min of this treatment, only fluorescent spots were present in root cells, accumulating either around nuclei or along cell walls. Six h later, in addition to some background fluorescence, dense but partially oriented oblique or longitudinal arrays of cortical MTs (CMTs) were found in most growing cells of the root apex. After 24 h of treatment, maize roots had adapted to the auxin, as inferred from the slowly recovering elongation rate and from the reassembly of a dense and well-ordered MT cytoskeleton which showed only slight deviations from that of the control root cells. Taxol pretreatment (100 microM, 24 h) prevented not only the rapid auxin-mediated disintegration of the MT cytoskeleton but also a reorientation of the CMT arrays, from transversal to longitudinal. The only tissue to show MTs in their cells throughout the auxin treatment was the epidermis. Significant resistance of transverse CMT arrays in these cells towards auxin was confirmed using a higher auxin concentration (100 microM, 24 h). The latter auxin dose also revealed inter-tissue-specific responses to auxin: outer cortical cell files reoriented their CMTs from the transversal to longitudinal orientation, whereas inner cortical cell files lost their MTs. This high auxin-mediated response, associated with the swelling of root apices, was abolished with the pretreatment of maize root with taxol.

Ionospheric holes made by ballistic missiles from North Korea detected with a Japanese dense GPS array

NASA Astrophysics Data System (ADS)

Ozeki, Masaru; Heki, Kosuke

2010-09-01

A dense array of global positioning system (GPS) receivers is a useful tool to study ionospheric disturbances. Here we report observations by a Japanese GPS array of ionospheric holes, i.e., localized electron depletion. They were made by neutral molecules in exhaust plumes (e.g., water) of ballistic missiles from North Korea, Taepodong-1 and -2, launched on 31 August, 1998, and 5 April, 2009, respectively. Negative anomaly of electron density emerged ˜6 min after the launches in the middle of the Japan Sea, and extended eastward along the missile tracks. By comparing the numerical simulation of electron depletion and the observed change in ionospheric total electron content, we suggest that the exhaust plumes from the Taepodong-2 second stage effused up to ˜1.5 × 1026 water molecules per second. The ionospheric hole signature was used to constrain the Taepodong-2 trajectory together with other information, e.g., coordinates of the launch pad, time and coordinates of the first stage splashdown, and height and time of the second stage passage over Japan. The Taepodong-2 is considered to have reached the ionospheric F region in ˜6 min, flown above northeastern Japan ˜7 min after the launch, and crashed to the Pacific Ocean without attaining the first astronautical velocity. The ionospheric hole in the 1998 Taepodong-1 launch was much less in size, but it is difficult to compare directly the thrusts of the two missiles due to uncertainty of the Taepodong-1 trajectory.

A new generation of ultra-dense optical I/O for silicon photonics

NASA Astrophysics Data System (ADS)

Wlodawski, Mitchell S.; Kopp, Victor I.; Park, Jongchul; Singer, Jonathan; Hubner, Eric E.; Neugroschl, Daniel; Chao, Norman; Genack, Azriel Z.

2014-03-01

In response to the optical packaging needs of a rapidly growing silicon photonics market, Chiral Photonics, Inc. (CPI) has developed a new generation of ultra-dense-channel, bi-directional, all-optical, input/output (I/O) couplers that bridge the data transport gap between standard optical fibers and photonic integrated circuits. These couplers, called Pitch Reducing Optical Fiber Arrays (PROFAs), provide a means to simultaneously match both the mode field and channel spacing (i.e. pitch) between an optical fiber array and a photonic integrated circuit (PIC). Both primary methods for optically interfacing with PICs, via vertical grating couplers (VGCs) and edge couplers, can be addressed with PROFAs. PROFAs bring the signal-carrying cores, either multimode or singlemode, of many optical fibers into close proximity within an all-glass device that can provide low loss coupling to on-chip components, including waveguides, gratings, detectors and emitters. Two-dimensional (2D) PROFAs offer more than an order of magnitude enhancement in channel density compared to conventional one-dimensional (1D) fiber arrays. PROFAs can also be used with low vertical profile solutions that simplify optoelectronic packaging while reducing PIC I/O real estate usage requirements. PROFA technology is based on a scalable production process for microforming glass preform assemblies as they are pulled through a small oven. An innovative fiber design, called the "vanishing core," enables tailoring the mode field along the length of the PROFA to meet the coupling needs of disparate waveguide technologies, such as fiber and onchip. Examples of single- and multi-channel couplers fabricated using this technology will be presented.

Image-guided ultrasound phased arrays are a disruptive technology for non-invasive therapy

NASA Astrophysics Data System (ADS)

Hynynen, Kullervo; Jones, Ryan M.

2016-09-01

Focused ultrasound offers a non-invasive way of depositing acoustic energy deep into the body, which can be harnessed for a broad spectrum of therapeutic purposes, including tissue ablation, the targeting of therapeutic agents, and stem cell delivery. Phased array transducers enable electronic control over the beam geometry and direction, and can be tailored to provide optimal energy deposition patterns for a given therapeutic application. Their use in combination with modern medical imaging for therapy guidance allows precise targeting, online monitoring, and post-treatment evaluation of the ultrasound-mediated bioeffects. In the past there have been some technical obstacles hindering the construction of large aperture, high-power, densely-populated phased arrays and, as a result, they have not been fully exploited for therapy delivery to date. However, recent research has made the construction of such arrays feasible, and it is expected that their continued development will both greatly improve the safety and efficacy of existing ultrasound therapies as well as enable treatments that are not currently possible with existing technology. This review will summarize the basic principles, current statures, and future potential of image-guided ultrasound phased arrays for therapy.

Image-guided ultrasound phased arrays are a disruptive technology for non-invasive therapy.

PubMed

Hynynen, Kullervo; Jones, Ryan M

2016-09-07

Focused ultrasound offers a non-invasive way of depositing acoustic energy deep into the body, which can be harnessed for a broad spectrum of therapeutic purposes, including tissue ablation, the targeting of therapeutic agents, and stem cell delivery. Phased array transducers enable electronic control over the beam geometry and direction, and can be tailored to provide optimal energy deposition patterns for a given therapeutic application. Their use in combination with modern medical imaging for therapy guidance allows precise targeting, online monitoring, and post-treatment evaluation of the ultrasound-mediated bioeffects. In the past there have been some technical obstacles hindering the construction of large aperture, high-power, densely-populated phased arrays and, as a result, they have not been fully exploited for therapy delivery to date. However, recent research has made the construction of such arrays feasible, and it is expected that their continued development will both greatly improve the safety and efficacy of existing ultrasound therapies as well as enable treatments that are not currently possible with existing technology. This review will summarize the basic principles, current statures, and future potential of image-guided ultrasound phased arrays for therapy.

Coherent array of branched filamentary scales along the wing margin of a small moth

NASA Astrophysics Data System (ADS)

Yoshida, Akihiro; Tejima, Shin; Sakuma, Masayuki; Sakamaki, Yositaka; Kodama, Ryuji

2017-04-01

In butterflies and moths, the wing margins are fringed with specialized scales that are typically longer than common scales. In the hindwings of some small moths, the posterior margins are fringed with particularly long filamentary scales. Despite the small size of these moth wings, these scales are much longer than those of large moths and butterflies. In the current study, photography of the tethered flight of a small moth, Phthorimaea operculella, revealed a wide array composed of a large number of long filamentary scales. This array did not become disheveled in flight, maintaining a coherent sheet-like structure during wingbeat. Examination of the morphology of individual scales revealed that each filamentary scale consists of a proximal stalk and distal branches. Moreover, not only long scales but also shorter scales of various lengths were found to coexist in each small section of the wing margin. Scale branches were ubiquitously and densely distributed within the scale array to form a mesh-like architecture similar to a nonwoven fabric. We propose that possible mechanical interactions among branched filamentary scales, mediated by these branches, may contribute to maintaining a coherent sheet-like structure of the scale array during wingbeat.

Image-guided ultrasound phased arrays are a disruptive technology for non-invasive therapy

PubMed Central

Hynynen, Kullervo; Jones, Ryan M.

2016-01-01

Focused ultrasound offers a non-invasive way of depositing acoustic energy deep into the body, which can be harnessed for a broad spectrum of therapeutic purposes, including tissue ablation, the targeting of therapeutic agents, and stem cell delivery. Phased array transducers enable electronic control over the beam geometry and direction, and can be tailored to provide optimal energy deposition patterns for a given therapeutic application. Their use in combination with modern medical imaging for therapy guidance allows precise targeting, online monitoring, and post-treatment evaluation of the ultrasound-mediated bioeffects. In the past there have been some technical obstacles hindering the construction of large aperture, high-power, densely-populated phased arrays and, as a result, they have not been fully exploited for therapy delivery to date. However, recent research has made the construction of such arrays feasible, and it is expected that their continued development will both greatly improve the safety and efficacy of existing ultrasound therapies as well as enable treatments that are not currently possible with existing technology. This review will summarize the basic principles, current statures, and future potential of image-guided ultrasound phased arrays for therapy. PMID:27494561

Source sparsity control of sound field reproduction using the elastic-net and the lasso minimizers.

PubMed

Gauthier, P-A; Lecomte, P; Berry, A

2017-04-01

Sound field reproduction is aimed at the reconstruction of a sound pressure field in an extended area using dense loudspeaker arrays. In some circumstances, sound field reproduction is targeted at the reproduction of a sound field captured using microphone arrays. Although methods and algorithms already exist to convert microphone array recordings to loudspeaker array signals, one remaining research question is how to control the spatial sparsity in the resulting loudspeaker array signals and what would be the resulting practical advantages. Sparsity is an interesting feature for spatial audio since it can drastically reduce the number of concurrently active reproduction sources and, therefore, increase the spatial contrast of the solution at the expense of a difference between the target and reproduced sound fields. In this paper, the application of the elastic-net cost function to sound field reproduction is compared to the lasso cost function. It is shown that the elastic-net can induce solution sparsity and overcomes limitations of the lasso: The elastic-net solves the non-uniqueness of the lasso solution, induces source clustering in the sparse solution, and provides a smoother solution within the activated source clusters.

Broadband implementation of coprime linear microphone arrays for direction of arrival estimation.

PubMed

Bush, Dane; Xiang, Ning

2015-07-01

Coprime arrays represent a form of sparse sensing which can achieve narrow beams using relatively few elements, exceeding the spatial Nyquist sampling limit. The purpose of this paper is to expand on and experimentally validate coprime array theory in an acoustic implementation. Two nested sparse uniform linear subarrays with coprime number of elements ( M and N) each produce grating lobes that overlap with one another completely in just one direction. When the subarray outputs are combined it is possible to retain the shared beam while mostly canceling the other superfluous grating lobes. In this way a small number of microphones ( N+M-1) creates a narrow beam at higher frequencies, comparable to a densely populated uniform linear array of MN microphones. In this work beampatterns are simulated for a range of single frequencies, as well as bands of frequencies. Narrowband experimental beampatterns are shown to correspond with simulated results even at frequencies other than the arrays design frequency. Narrowband side lobe locations are shown to correspond to the theoretical values. Side lobes in the directional pattern are mitigated by increasing bandwidth of analyzed signals. Direction of arrival estimation is also implemented for two simultaneous noise sources in a free field condition.

Free-breathing cine DENSE MRI using phase cycling with matchmaking and stimulated-echo image-based navigators.

PubMed

Cai, Xiaoying; Epstein, Frederick H

2018-04-01

This study aimed to develop a self-navigated method for free-breathing spiral cine displacement encoding with stimulated echoes (DENSE), a myocardial strain imaging technique that uses phase-cycling for artifact suppression. The method needed to address 2 consequences of motion for DENSE: striping artifacts from incomplete suppression of the T 1 -relaxation echo and blurring. The method identifies phase-cycled spiral interleaves at matched respiratory phases by minimizing the residual signal due to T 1 relaxation after phase-cycling subtraction. Next, the method reconstructs image-based navigators from matched phase-cycled interleaves that are comprised of the stimulated echo (ste-iNAVs). Ste-iNAVs are used for motion estimation and compensation of k-space data. The method was demonstrated in phantoms and compared to diaphragm-based navigator (dNAV) and conventional iNAV (c-iNAV) methods for the reconstruction of free-breathing volunteer data sets (N = 10). Phantom experiments demonstrated that the proposed method removes striping artifacts and blurring due to motion. Volunteer results showed that respiratory motion measured by ste-iNAVs was better correlated than c-iNAVs to dNAV data (R 2  = 0.82 ± 0.03 vs. 0.70 ± 0.05, P < 0.05). Match-making reconstructions of free-breathing data sets achieved lower residual T 1 -relaxation echo energy (1.04 ± 0.01 vs. 1.18 ± 0.04 for dNAV and 1.18 ± 0.03 for c-iNAV, P < 0.05), higher apparent SNR (11.93 ± 1.05 vs. 10.68 ± 1.06 for dNAV and 10.66 ± 0.99 for c-iNAV, P < 0.05), and better phase quality (0.147 ± 0.012 vs. 0.166 ± 0.017 for dNAV, P = 0.06, and 0.168 ± 0.015 for c-iNAV, P < 0.05) than dNAV and c-iNAV methods. For free-breathing cine DENSE, the proposed method addresses both types of breathing-induced artifacts and provides better quality images than conventional dNAV and iNAV methods. © 2018 International Society for Magnetic Resonance in Medicine.

Dynamics of conical wire array Z-pinch implosions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ampleford, D. J.; Lebedev, S. V.; Bland, S. N.

2007-10-15

A modification of the wire array Z pinch, the conical wire array, has applications to the understanding of wire array implosions and potentially to pulse shaping relevant to inertial confinement fusion. Results are presented from imploding conical wire array experiments performed on university scale 1 MA generators--the MAGPIE generator (1 MA, 240 ns) at Imperial College London [I. H. Mitchell et al., Rev. Sci Instrum. 67, 1533 (1996)] and the Nevada Terawatt Facility's Zebra generator (1 MA, 100 ns) at the University of Nevada, Reno [B. Bauer et al., in Dense Z-Pinches, edited by N. Pereira, J. Davis, and P.more » Pulsifer (AIP, New York, 1997), Vol. 409, p. 153]. This paper will discuss the implosion dynamics of conical wire arrays. Data indicate that mass ablation from the wires in this complex system can be reproduced with a rocket model with fixed ablation velocity. Modulations in the ablated plasma are present, the wavelength of which is invariant to a threefold variation in magnetic field strength. The axial variation in the array leads to a zippered precursor column formation. An initial implosion of a magnetic bubble near the cathode is followed by the implosion zippering upwards. Spectroscopic data demonstrating a variation of plasma parameters (e.g., electron temperature) along the Z-pinch axis is discussed, and experimental data are compared to magnetohydrodynamic simulations.« less

Cochlear's unique electrode portfolio now and in the future.

PubMed

von Wallenberg, E; Briggs, R

2014-05-01

To review Cochlear's electrode portfolio and discuss the merits of current and future straight and perimodiolar electrode arrays. To present an update on implant reliability. Performance and hearing preservation data from studies involving the Slim Straight (CI422), Hybrid L24 and Contour Advance electrode array were reviewed. While several studies in past found little difference in performance outcomes between subjects implanted with perimodiolar and straight arrays, recent studies demonstrated that proximity to the modiolus is correlated with better performance. Hearing threshold increase was lowest with the Hybrid L24, closely followed by the slim straight array and was largest with the Contour Advance array. The CI24RE receiver-stimulator used for the three arrays had a cumulative survival of 99% at eight years post implantation. Combining the hearing preservation benefits of slim straight arrays with perimodiolar proximity is the design objective of Cochlear's next generation electrodes.

Vaccination with Irradiated Autologous Melanoma Cells Engineered to Secrete Human Granulocyte--Macrophage Colony-Stimulating Factor Generates Potent Antitumor Immunity in Patients with Metastatic Melanoma

NASA Astrophysics Data System (ADS)

Soiffer, Robert; Lynch, Thomas; Mihm, Martin; Jung, Ken; Rhuda, Catherine; Schmollinger, Jan C.; Hodi, F. Stephen; Liebster, Laura; Lam, Prudence; Mentzer, Steven; Singer, Samuel; Tanabe, Kenneth K.; Benedict Cosimi, A.; Duda, Rosemary; Sober, Arthur; Bhan, Atul; Daley, John; Neuberg, Donna; Parry, Gordon; Rokovich, Joseph; Richards, Laurie; Drayer, Jan; Berns, Anton; Clift, Shirley; Cohen, Lawrence K.; Mulligan, Richard C.; Dranoff, Glenn

1998-10-01

We conducted a Phase I clinical trial investigating the biologic activity of vaccination with irradiated autologous melanoma cells engineered to secrete human granulocyte--macrophage colony-stimulating factor in patients with metastatic melanoma. Immunization sites were intensely infiltrated with T lymphocytes, dendritic cells, macrophages, and eosinophils in all 21 evaluable patients. Although metastatic lesions resected before vaccination were minimally infiltrated with cells of the immune system in all patients, metastatic lesions resected after vaccination were densely infiltrated with T lymphocytes and plasma cells and showed extensive tumor destruction (at least 80%), fibrosis, and edema in 11 of 16 patients examined. Antimelanoma cytotoxic T cell and antibody responses were associated with tumor destruction. These results demonstrate that vaccination with irradiated autologous melanoma cells engineered to secrete granulocyte--macrophage colony-stimulating factor stimulates potent antitumor immunity in humans with metastatic melanoma.

Experimental damage detection of wind turbine blade using thin film sensor array

NASA Astrophysics Data System (ADS)

Downey, Austin; Laflamme, Simon; Ubertini, Filippo; Sarkar, Partha

2017-04-01

Damage detection of wind turbine blades is difficult due to their large sizes and complex geometries. Additionally, economic restraints limit the viability of high-cost monitoring methods. While it is possible to monitor certain global signatures through modal analysis, obtaining useful measurements over a blade's surface using off-the-shelf sensing technologies is difficult and typically not economical. A solution is to deploy dedicated sensor networks fabricated from inexpensive materials and electronics. The authors have recently developed a novel large-area electronic sensor measuring strain over very large surfaces. The sensing system is analogous to a biological skin, where local strain can be monitored over a global area. In this paper, we propose the utilization of a hybrid dense sensor network of soft elastomeric capacitors to detect, localize, and quantify damage, and resistive strain gauges to augment such dense sensor network with high accuracy data at key locations. The proposed hybrid dense sensor network is installed inside a wind turbine blade model and tested in a wind tunnel to simulate an operational environment. Damage in the form of changing boundary conditions is introduced into the monitored section of the blade. Results demonstrate the ability of the hybrid dense sensor network, and associated algorithms, to detect, localize, and quantify damage.

Close-field electroporation gene delivery using the cochlear implant electrode array enhances the bionic ear.

PubMed

Pinyon, Jeremy L; Tadros, Sherif F; Froud, Kristina E; Y Wong, Ann C; Tompson, Isabella T; Crawford, Edward N; Ko, Myungseo; Morris, Renée; Klugmann, Matthias; Housley, Gary D

2014-04-23

The cochlear implant is the most successful bionic prosthesis and has transformed the lives of people with profound hearing loss. However, the performance of the "bionic ear" is still largely constrained by the neural interface itself. Current spread inherent to broad monopolar stimulation of the spiral ganglion neuron somata obviates the intrinsic tonotopic mapping of the cochlear nerve. We show in the guinea pig that neurotrophin gene therapy integrated into the cochlear implant improves its performance by stimulating spiral ganglion neurite regeneration. We used the cochlear implant electrode array for novel "close-field" electroporation to transduce mesenchymal cells lining the cochlear perilymphatic canals with a naked complementary DNA gene construct driving expression of brain-derived neurotrophic factor (BDNF) and a green fluorescent protein (GFP) reporter. The focusing of electric fields by particular cochlear implant electrode configurations led to surprisingly efficient gene delivery to adjacent mesenchymal cells. The resulting BDNF expression stimulated regeneration of spiral ganglion neurites, which had atrophied 2 weeks after ototoxic treatment, in a bilateral sensorineural deafness model. In this model, delivery of a control GFP-only vector failed to restore neuron structure, with atrophied neurons indistinguishable from unimplanted cochleae. With BDNF therapy, the regenerated spiral ganglion neurites extended close to the cochlear implant electrodes, with localized ectopic branching. This neural remodeling enabled bipolar stimulation via the cochlear implant array, with low stimulus thresholds and expanded dynamic range of the cochlear nerve, determined via electrically evoked auditory brainstem responses. This development may broadly improve neural interfaces and extend molecular medicine applications.

Rapid prototyping of flexible intrafascicular electrode arrays by picosecond laser structuring

NASA Astrophysics Data System (ADS)

Mueller, Matthias; de la Oliva, Natalia; del Valle, Jaume; Delgado-Martínez, Ignacio; Navarro, Xavier; Stieglitz, Thomas

2017-12-01

Objective. Interfacing the peripheral nervous system can be performed with a large variety of electrode arrays. However, stimulating and recording a nerve while having a reasonable amount of channels limits the number of available systems. Translational research towards human clinical trial requires device safety and biocompatibility but would benefit from design flexibility in the development process to individualize probes. Approach. We selected established medical grade implant materials like precious metals and Parylene C to develop a rapid prototyping process for novel intrafascicular electrode arrays using a picosecond laser structuring. A design for a rodent animal model was developed in conjunction with an intrafascicular implantation strategy. Electrode characterization and optimization was performed first in saline solution in vitro before performance and biocompatibility were validated in sciatic nerves of rats in chronic implantation. Main results. The novel fabrication process proved to be suitable for prototyping and building intrafascicular electrode arrays. Electrochemical properties of the electrode sites were enhanced and tested for long-term stability. Chronic implantation in the sciatic nerve of rats showed good biocompatibility, selectivity and stable stimulation thresholds. Significance. Established medical grade materials can be used for intrafascicular nerve electrode arrays when laser structuring defines structure size in the micro-scale. Design flexibility reduces re-design cycle time and material certificates are beneficial support for safety studies on the way to clinical trials.

An Embedded 4-Channel Receive-Only RF Coil Array for fMRI Experiments of the Somatosensory Pathway in Conscious Awake Marmosets at 7T

PubMed Central

Papoti, Daniel; Yen, Cecil Chern-Chyi; Mackel, Julie B.; Merkle, Hellmut; Silva, Afonso C.

2014-01-01

Functional Magnetic Resonance Imaging (fMRI) has established itself as the main research tool in neuroscience and brain cognitive research. The common marmoset (Callithrix jacchus) is a non-human primate model of increasing interest in biomedical research. However, commercial MRI coils for marmosets are not generally available. The present work describes the design and construction of a 4-channel receive-only surface RF coil array with excellent signal-to-noise ratio (SNR) specifically optimized for fMRI experiments in awake marmosets in response to somatosensory stimulation. The array was designed as part of a helmet-based head restraint system used to prevent motion during the scans. High SNR was obtained by building the coil array using a thin and flexible substrate glued to the inner surface of the restraint helmet, so as to minimize the distance between the array elements and the somatosensory cortex. Decoupling between coil elements was achieved by partial geometrical overlapping and by connecting them to home-built low input impedance preamplifiers. In vivo images show excellent coverage of the brain cortical surface with high sensitivity near the somatosensory cortex. Embedding the coil elements within the restraint helmet allowed fMRI data in response to somatosensory stimulation to be collected with high sensitivity and reproducibility in conscious, awake marmosets. PMID:23696219

Multiple View Zenith Angle Observations of Reflectance From Ponderosa Pine Stands

NASA Technical Reports Server (NTRS)

Johnson, Lee F.; Lawless, James G. (Technical Monitor)

1994-01-01

Reflectance factors (RF(lambda)) from dense and sparse ponderosa pine (Pinus ponderosa) stands, derived from radiance data collected in the solar principal plane by the Advanced Solid-State Array Spectro-radiometer (ASAS), were examined as a function of view zenith angle (theta(sub v)). RF(lambda) was maximized with theta(sub v) nearest the solar retrodirection, and minimized near the specular direction throughout the ASAS spectral region. The dense stand had much higher RF anisotropy (ma)dmurn RF is minimum RF) in the red region than did the sparse stand (relative differences of 5.3 vs. 2.75, respectively), as a function of theta(sub v), due to the shadow component in the canopy. Anisotropy in the near-infrared (NIR) was more similar between the two stands (2.5 in the dense stand and 2.25 in the sparse stand); the dense stand exhibited a greater hotspot effect than 20 the sparse stand in this spectral region. Two common vegetation transforms, the NIR/red ratio and the normalized difference vegetation index (NDVI), both showed a theta(sub v) dependence for the dense stand. Minimum values occurred near the retrodirection and maximum values occurred near the specular direction. Greater relative differences were noted for the NIR/red ratio (2.1) than for the NDVI (1.3). The sparse stand showed no obvious dependence on theta(sub v) for either transform, except for slightly elevated values toward the specular direction.

SU-F-T-321: The Effect of an Electromagnetic Array Used for Patient Localization and Tumor Tracking On OSLD in Vivo Dosimetry

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rea, A; Kuruvilla, A; Gill, G

Purpose: The purpose of this study was to observe the effect of an electromagnetic array used for patient localization and tumor tracking on optically-stimulated luminescent in-vivo dosimetry. Methods: A linear accelerator equipped with four photon energies was used to irradiate optically stimulated luminescent dosimeters (OSLDs) at the respective dmax depths and in the buildup region, with and without the presence of an electromagnetic array used for tumor tracking and patient localization. The OSLDs were placed on solid water slabs under 5 mm bolus and on each face of an octagonal phantom, and irradiated using both static beam and arc geometry,more » with and without the electromagnetic array under our setup. The electromagnetic array was placed 6 cm above the phantom to coincide with similar distances used during patient treatment. Ionization chamber measurements in a water phantom were also taken initially for comparison with the simple geometry OSLD measurements and published data. Results: Under simple geometry, a negligible change was observed at dmax for all energies when the electromagnetic array was placed over the setup. When measuring at five millimeter depth, increases of 1.3/3.1/16/18% were observed for energies 4X/6X/10X/15X respectively when the electromagnetic array was in place. Measurements using the octagonal phantom yielded scattered results for the lateral and posterior oblique fields, and showed increases in dose to the OSLDs placed on the anterior and lateral anterior faces of the phantom. Conclusion: Placing the electromagnetic array very close to the patient’s surface acts as a beam spoiler in the buildup region (at 5 mm depth), which in turn causes an increase in the measured dose reading of the OSLD. This increase in dose is more pronounced when the OSLD is placed directly underneath the electromagnetic array than off to one side or the other.« less

Two- to three-dimensional crossover in a dense electron liquid in silicon

NASA Astrophysics Data System (ADS)

Matmon, Guy; Ginossar, Eran; Villis, Byron J.; Kölker, Alex; Lim, Tingbin; Solanki, Hari; Schofield, Steven R.; Curson, Neil J.; Li, Juerong; Murdin, Ben N.; Fisher, Andrew J.; Aeppli, Gabriel

2018-04-01

Doping of silicon via phosphine exposures alternating with molecular beam epitaxy overgrowth is a path to Si:P substrates for conventional microelectronics and quantum information technologies. The technique also provides a well-controlled material for systematic studies of two-dimensional lattices with a half-filled band. We show here that for a dense (ns=2.8 ×1014 cm-2) disordered two-dimensional array of P atoms, the full field magnitude and angle-dependent magnetotransport is remarkably well described by classic weak localization theory with no corrections due to interaction. The two- to three-dimensional crossover seen upon warming can also be interpreted using scaling concepts developed for anistropic three-dimensional materials, which work remarkably except when the applied fields are nearly parallel to the conducting planes.

VizieR Online Data Catalog: Virial analysis of the dense cores in Orion A (Kirk+, 2017)

NASA Astrophysics Data System (ADS)

Kirk, H.; Friesen, R. K.; Pineda, J. E.; Rosolowsky, E.; Offner, S. S. R.; Matzner, C. D.; Myers, P. C.; di, Francesco J.; Caselli, P.; Alves, F. O.; Chacon-Tanarro, A.; Chen, H.-H.; Chen, M. C.-Y.; Keown, J.; Punanova, A.; Seo, Y. M.; Shirley, Y.; Ginsburg, A.; Hall, C.; Singh, A.; Arce, H. G.; Goodman, A. A.; Martin, P.; Redaelli, E.

2018-05-01

NH3 observations were obtained through the Green Bank Ammonia (GAS), a large project to map the ammonia (1,1), (2,2), and (3,3) rotation-inversion transitions across the high-extinction regions of nearby Gould Belt molecular clouds using the Green Bank Telescope's K-band Focal Plane Array. The survey strategy, data reduction procedure, and basic data properties are described in detail in Friesen+ (2017ApJ...843...63F). The spatial resolution is 32" (0.064pc), while the spectral resolution is 0.07km/s. For our analysis, we use the dense core catalog presented in Lane+ (2016ApJ...833...44L), which covers the entire Orion A complex with the JCMT SCUBA-2 850um. See section 2.2. (1 data file).

Nanopatterning of Si(001) for bottom-up fabrication of nanostructures.

PubMed

Hu, Yanfang; Kalachahi, Hedieh Hosseinzadeh; Das, Amal K; Koch, Reinhold

2012-04-27

The epitaxial growth of Si on Si(001) under conditions at which the (2 × n) superstructure is forming has been investigated by scanning tunneling microscopy and Monte Carlo simulations. Our experiments reveal a periodic change of the surface morphology with the surface coverage of Si. A regular (2 × n) stripe pattern is observed at coverages of 0.7-0.9 monolayers that periodically alternates with less dense surface structures at lower Si surface coverages. The MC simulations show that the growth of Si is affected by step-edge barriers, which favors the formation of a rather uniform two-dimensional framework-like configuration. Subsequent deposition of Ge onto the (2 × n) stripe pattern yields a dense array of small Ge nanostructures.

Characterising the Dense Molecular Gas in Exceptional Local Galaxies

NASA Astrophysics Data System (ADS)

Tunnard, Richard C. A.

2016-08-01

The interferometric facilities now coming online (the Atacama Large Millimetre Array (ALMA) and the NOrthern Extended Millimeter Array (NOEMA)) and those planned for the coming decade (the Next Generation Very Large Array (ngVLA) and the Square Kilometre Array (SKA)) in the radio to sub-millimetre regimes are opening a window to the molecular gas in high-redshift galaxies. However, our understanding of similar galaxies in the local universe is still far from complete and the data analysis techniques and tools needed to interpret the observations in consistent and comparable ways are yet to be developed. I first describe the Monte Carlo Markov Chain (MCMC) script developed to empower a public radiative transfer code. I characterise both the public code and MCMC script, including an exploration of the effect of observing molecular lines at high redshift where the Cosmic Microwave Background (CMB) can provide a significant background, as well as the effect this can have on well-known local correlations. I present two studies of ultraluminous infrared galaxies (ULIRGs) in the local universe making use of literature and collaborator data. In the first of these, NGC6240, I use the wealth of available data and the geometry of the source to develop a multi-phase, multi-species model, finding evidence for a complex medium of hot diffuse and cold dense gas in pressure equilibrium. Next, I study the prototypical ULIRG Arp 220; an extraordinary galaxy rendered especially interesting by the controversy over the power source of the western of the two merger nuclei and its immense luminosity and dust obscuration. Using traditional grid based methods I explore the molecular gas conditions within the nuclei and find evidence for chemical differentiation between the two nuclei, potentially related to the obscured power source. Finally, I investigate the potential evolution of proto-clusters over cosmic time with sub-millimetre observations of 14 radio galaxies, unexpectedly finding little to no evidence for cluster evolution.

Active-Source Seismic Tomography at Bradys Geothermal Field, Nevada, with Dense Nodal and Fiber-Optic Seismic Arrays

NASA Astrophysics Data System (ADS)

Thurber, C. H.; Parker, L.; Li, P.; Fratta, D.; Zeng, X.; Feigl, K. L.; Ak, E.; Lord, N.

2017-12-01

We deployed a dense seismic array to image the shallow structure in the injection area of the Brady Hot Springs geothermal site in Nevada. The array was composed of 238 5 Hz, three-component nodal instruments and 8,700 m of distributed acoustic sensing (DAS) fiber-optic cable installed in surface trenches plus about 400 m installed in a borehole. The geophone array had about 60 m instrument spacing in the target zone, whereas DAS channel separations were about 1 m. The acquisition systems provided 15 days of continuous records including active source and ambient noise signals. A large vibroseis truck (T-Rex) was operated at 196 locations, exciting a swept-frequency signal from 5 to 80 Hz over 20 seconds using three vibration modes. Sweeps were repeated up to four times during different modes of geothermal plant operation: normal operation, shut-down, high and oscillatory injection and production, and normal operation again. The cross-correlation method was utilized to remove the sweep signal from the geophone records. The first P arrivals were automatically picked from the cross-correlation functions using a combination of methods, and the travel times were used to invert for the 3D P-wave velocity structure. Models with 100 m and 50 m horizontal node spacing were obtained, with vertical node spacing of 10 to 50 m. The travel time data were fit to about 30 ms, close to our estimated picking uncertainty. We will present our 3D Vp model and the result of our search for measurable temporal changes, along with preliminary results for a 3D Vs model. The work presented herein was funded in part by the Office of Energy Efficiency and Renewable Energy (EERE), U.S. Department of Energy, under Award Number DE-EE0006760.

Transparent, conformable, active multielectrode array using organic electrochemical transistors

PubMed Central

Lee, Wonryung; Kim, Dongmin; Matsuhisa, Naoji; Nagase, Masae; Sekino, Masaki; Malliaras, George G.; Yokota, Tomoyuki; Someya, Takao

2017-01-01

Mechanically flexible active multielectrode arrays (MEA) have been developed for local signal amplification and high spatial resolution. However, their opaqueness limited optical observation and light stimulation during use. Here, we show a transparent, ultraflexible, and active MEA, which consists of transparent organic electrochemical transistors (OECTs) and transparent Au grid wirings. The transparent OECT is made of Au grid electrodes and has shown comparable performance with OECTs with nontransparent electrodes/wirings. The transparent active MEA realizes the spatial mapping of electrocorticogram electrical signals from an optogenetic rat with 1-mm spacing and shows lower light artifacts than noise level. Our active MEA would open up the possibility of precise investigation of a neural network system with direct light stimulation. PMID:28923928

The AlpArray Seismic Network: current status and next steps

NASA Astrophysics Data System (ADS)

Hetényi, György; Molinari, Irene; Clinton, John; Kissling, Edi

2016-04-01

The AlpArray initiative (http://www.alparray.ethz.ch) is a large-scale European collaboration to study the entire Alpine orogen at high resolution and in 3D with a large variety of geoscientific methods. The core element of the initiative is an extensive and dense broadband seismological network, the AlpArray Seismic Network (AASN), which complements the permanent seismological stations to ensure homogeneous coverage of the greater Alpine area. The some 260 temporary stations of the AlpArray Seismic Network are operated as a joint effort by a number of institutions from Austria, Bosnia-Herzegovina, Croatia, Czech Republic, France, Germany, Hungary, Italy, Slovakia and Switzerland. The first stations were installed in Spring 2015 and the full AASN is planned to be operational by early Summer 2016. In this poster we present the actual status of the deployment, the effort undertaken by the contributing groups, station performance, typical noise levels, best practices in installation as well as in data management, often encountered challenges, and planned next steps including the deployment of ocean bottom seismometers in the Ligurian Sea.

An image-based array trigger for imaging atmospheric Cherenkov telescope arrays

NASA Astrophysics Data System (ADS)

Dickinson, Hugh; Krennrich, Frank; Weinstein, Amanda; Eisch, Jonathan; Byrum, Karen; Anderson, John; Drake, Gary

2018-05-01

It is anticipated that forthcoming, next generation, atmospheric Cherenkov telescope arrays will include a number of medium-sized telescopes that are constructed using a dual-mirror Schwarzschild-Couder configuration. These telescopes will sample a wide (8 °) field of view using a densely pixelated camera comprising over 104 individual readout channels. A readout frequency congruent with the expected single-telescope trigger rates would result in substantial data rates. To ameliorate these data rates, a novel, hardware-level Distributed Intelligent Array Trigger (DIAT) is envisioned. A copy of the DIAT operates autonomously at each telescope and uses reduced resolution imaging data from a limited subset of nearby telescopes to veto events prior to camera readout and any subsequent network transmission of camera data that is required for centralized storage or aggregation. We present the results of Monte-Carlo simulations that evaluate the efficacy of a "Parallax width" discriminator that can be used by the DIAT to efficiently distinguish between genuine gamma-ray initiated events and unwanted background events that are initiated by hadronic cosmic rays.

The KASCADE-Grande experiment

NASA Astrophysics Data System (ADS)

Apel, W. D.; Arteaga, J. C.; Badea, A. F.; Bekk, K.; Bertaina, M.; Blümer, J.; Bozdog, H.; Brancus, I. M.; Buchholz, P.; Cantoni, E.; Chiavassa, A.; Cossavella, F.; Daumiller, K.; de Souza, V.; Di Pierro, F.; Doll, P.; Engel, R.; Engler, J.; Finger, M.; Fuhrmann, D.; Ghia, P. L.; Gils, H. J.; Glasstetter, R.; Grupen, C.; Haungs, A.; Heck, D.; Hörandel, J. R.; Huege, T.; Isar, P. G.; Kampert, K.-H.; Kang, D.; Kickelbick, D.; Klages, H. O.; Link, K.; łuczak, P.; Ludwig, M.; Mathes, H. J.; Mayer, H. J.; Melissas, M.; Milke, J.; Mitrica, B.; Morello, C.; Navarra, G.; Nehls, S.; Oehlschläger, J.; Ostapchenko, S.; Over, S.; Palmieri, N.; Petcu, M.; Pierog, T.; Rebel, H.; Roth, M.; Schieler, H.; Schröder, F.; Sima, O.; Stümpert, M.; Toma, G.; Trinchero, G. C.; Ulrich, H.; Weindl, A.; Wochele, J.; Wommer, M.; Zabierowski, J.

2010-08-01

KASCADE-Grande is the enlargement of the KASCADE extensive air shower detector, realized to expand the cosmic ray studies from the previous 10 14-10 17 eV primary energy range to 10 18 eV. This is performed by extending the area covered by the KASCADE electromagnetic array from 200×200 to 700×700 m 2 by means of 37 scintillator detector stations of 10 m 2 area each. This new array is named Grande and provides measurements of the all-charged particle component of extensive air showers ( Nch), while the original KASCADE array particularly provides information on the muon content (Nμ). Additional dense compact detector set-ups being sensitive to energetic hadrons and muons are used for data consistency checks and calibration purposes. The performance of the Grande array and its integration into the entire experimental complex is discussed. It is demonstrated that the overall observable resolutions are adequate to meet the physical requirements of the measurements, i.e. primary energy spectrum and elemental composition studies in the primary cosmic ray energy range of 10 16-10 18 eV.

Ferromagnetic resonance in low interacting permalloy nanowire arrays

DOE Office of Scientific and Technical Information (OSTI.GOV)

Raposo, V.; Zazo, M.; Flores, A. G.

2016-04-14

Dipolar interactions on magnetic nanowire arrays have been investigated by various techniques. One of the most powerful techniques is the ferromagnetic resonance spectroscopy, because the resonance field depends directly on the anisotropy field strength and its frequency dependence. In order to evaluate the influence of magnetostatic dipolar interactions among ferromagnetic nanowire arrays, several densely packed hexagonal arrays of NiFe nanowires have been prepared by electrochemical deposition filling self-ordered nanopores of alumina membranes with different pore sizes but keeping the same interpore distance. Nanowires’ diameter was changed from 90 to 160 nm, while the lattice parameter was fixed to 300 nm, which wasmore » achieved by carefully reducing the pore diameter by means of Atomic Layer Deposition of conformal Al{sub 2}O{sub 3} layers on the nanoporous alumina templates. Field and frequency dependence of ferromagnetic resonance have been studied in order to obtain the dispersion diagram which gives information about anisotropy, damping factor, and gyromagnetic ratio. The relationship between resonance frequency and magnetic field can be explained by the roles played by the shape anisotropy and dipolar interactions among the ferromagnetic nanowires.« less

Synthesis and characterization of barium silicide (BaSi2) nanowire arrays for potential solar applications.

PubMed

Pokhrel, Ankit; Samad, Leith; Meng, Fei; Jin, Song

2015-11-07

In order to utilize nanostructured materials for potential solar and other energy-harvesting applications, scalable synthetic techniques for these materials must be developed. Herein we use a vapor phase conversion approach to synthesize nanowire (NW) arrays of semiconducting barium silicide (BaSi2) in high yield for the first time for potential solar applications. Dense arrays of silicon NWs obtained by metal-assisted chemical etching were converted to single-crystalline BaSi2 NW arrays by reacting with Ba vapor at about 930 °C. Structural characterization by X-ray diffraction and high-resolution transmission electron microscopy confirm that the converted NWs are single-crystalline BaSi2. The optimal conversion reaction conditions allow the phase-pure synthesis of BaSi2 NWs that maintain the original NW morphology, and tuning the reaction parameters led to a controllable synthesis of BaSi2 films on silicon substrates. The optical bandgap and electrochemical measurements of these BaSi2 NWs reveal a bandgap and carrier concentrations comparable to previously reported values for BaSi2 thin films.

Focal activation of primary visual cortex following supra-choroidal electrical stimulation of the retina: Intrinsic signal imaging and linear model analysis.

PubMed

Cloherty, Shaun L; Hietanen, Markus A; Suaning, Gregg J; Ibbotson, Michael R

2010-01-01

We performed optical intrinsic signal imaging of cat primary visual cortex (Area 17 and 18) while delivering bipolar electrical stimulation to the retina by way of a supra-choroidal electrode array. Using a general linear model (GLM) analysis we identified statistically significant (p < 0.01) activation in a localized region of cortex following supra-threshold electrical stimulation at a single retinal locus. (1) demonstrate that intrinsic signal imaging combined with linear model analysis provides a powerful tool for assessing cortical responses to prosthetic stimulation, and (2) confirm that supra-choroidal electrical stimulation can achieve localized activation of the cortex consistent with focal activation of the retina.

Thermally Stabilized Transmit/Receive Modules

NASA Technical Reports Server (NTRS)

Hoffman, James; DelCastillo, Linda; Miller, Jennifer; Birur, Gaj

2011-01-01

RF-hybrid technologies enable smaller packaging and mass reduction in radar instruments, especially for subsystems with dense electronics, such as electronically steered arrays. We are designing thermally stabilized RF-hybrid T/R modules using new materials for improved thermal performance of electronics. We are combining advanced substrate and housing materials with a thermal reservoir material, and develop new packaging techniques to significantly improve thermal-cycling reliability and performance stability over temperature.

RADIO TRANSIENTS FROM ACCRETION-INDUCED COLLAPSE OF WHITE DWARFS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Moriya, Takashi J., E-mail: takashi.moriya@nao.ac.jp

2016-10-20

We investigate observational properties of accretion-induced collapse (AIC) of white dwarfs (WDs) in radio frequencies. If AIC is triggered by accretion from a companion star, a dense circumstellar medium can be formed around the progenitor system. Then, the ejecta from AIC collide with the dense circumstellar medium, creating a strong shock. The strong shock can produce synchrotron emission that can be observed in radio frequencies. Even if AIC occurs as a result of WD mergers, we argue that AIC may cause fast radio bursts (FRBs) if a certain condition is satisfied. If AIC forms neutron stars (NSs) that are somore » massive that rotation is required to support themselves (i.e., supramassive NSs), the supramassive NSs may immediately lose their rotational energy by the r-mode instability and collapse to black holes. If the collapsing supramassive NSs are strongly magnetized, they may emit FRBs, as previously proposed. The AIC radio transients from single-degenerate systems may be detected in future radio transient surveys like the Very Large Array Sky Survey or the Square Kilometer Array transient survey. Because AIC has been proposed as a source of gravitational waves (GWs), GWs from AIC may be accompanied by radio-bright transients that can be used to confirm the AIC origin of observed GWs.« less

Design and engineering of photosynthetic light-harvesting and electron transfer using length, time, and energy scales.

PubMed

Noy, Dror; Moser, Christopher C; Dutton, P Leslie

2006-02-01

Decades of research on the physical processes and chemical reaction-pathways in photosynthetic enzymes have resulted in an extensive database of kinetic information. Recently, this database has been augmented by a variety of high and medium resolution crystal structures of key photosynthetic enzymes that now include the two photosystems (PSI and PSII) of oxygenic photosynthetic organisms. Here, we examine the currently available structural and functional information from an engineer's point of view with the long-term goal of reproducing the key features of natural photosystems in de novo designed and custom-built molecular solar energy conversion devices. We find that the basic physics of the transfer processes, namely, the time constraints imposed by the rates of incoming photon flux and the various decay processes allow for a large degree of tolerance in the engineering parameters. Moreover, we find that the requirements to guarantee energy and electron transfer rates that yield high efficiency in natural photosystems are largely met by control of distance between chromophores and redox cofactors. Thus, for projected de novo designed constructions, the control of spatial organization of cofactor molecules within a dense array is initially given priority. Nevertheless, constructions accommodating dense arrays of different cofactors, some well within 1 nm from each other, still presents a significant challenge for protein design.

Pairwise graphical models for structural health monitoring with dense sensor arrays

NASA Astrophysics Data System (ADS)

Mohammadi Ghazi, Reza; Chen, Justin G.; Büyüköztürk, Oral

2017-09-01

Through advances in sensor technology and development of camera-based measurement techniques, it has become affordable to obtain high spatial resolution data from structures. Although measured datasets become more informative by increasing the number of sensors, the spatial dependencies between sensor data are increased at the same time. Therefore, appropriate data analysis techniques are needed to handle the inference problem in presence of these dependencies. In this paper, we propose a novel approach that uses graphical models (GM) for considering the spatial dependencies between sensor measurements in dense sensor networks or arrays to improve damage localization accuracy in structural health monitoring (SHM) application. Because there are always unobserved damaged states in this application, the available information is insufficient for learning the GMs. To overcome this challenge, we propose an approximated model that uses the mutual information between sensor measurements to learn the GMs. The study is backed by experimental validation of the method on two test structures. The first is a three-story two-bay steel model structure that is instrumented by MEMS accelerometers. The second experimental setup consists of a plate structure and a video camera to measure the displacement field of the plate. Our results show that considering the spatial dependencies by the proposed algorithm can significantly improve damage localization accuracy.

TREMOR: A wireless MEMS accelerograph for dense arrays

USGS Publications Warehouse

Evans, J.R.; Hamstra, R.H.; Kundig, C.; Camina, P.; Rogers, J.A.

2005-01-01

The ability of a strong-motion network to resolve wavefields can be described on three axes: frequency, amplitude, and space. While the need for spatial resolution is apparent, for practical reasons that axis is often neglected. TREMOR is a MEMS-based accelerograph using wireless Internet to minimize lifecycle cost. TREMOR instruments can economically augment traditional ones, residing between them to improve spatial resolution. The TREMOR instrument described here has dynamic range of 96 dB between ??2 g, or 102 dB between ??4 g. It is linear to ???1% of full scale (FS), with a response function effectively shaped electronically. We developed an economical, very low noise, accurate (???1%FS) temperature compensation method. Displacement is easily recovered to 10-cm accuracy at full bandwidth, and better with care. We deployed prototype instruments in Oakland, California, beginning in 1998, with 13 now at mean spacing of ???3 km - one of the most densely instrumented urban centers in the United States. This array is among the quickest in returning (PGA, PGV, Sa) vectors to ShakeMap, ???75 to 100 s. Some 13 events have been recorded. A ShakeMap and an example of spatial variability are shown. Extensive tests of the prototypes for a commercial instrument are described here and in a companion paper. ?? 2005, Earthquake Engineering Research Institute.

Dynamic Detection of Spinal Cord Position During Postural Changes Using Near-Infrared Reflectometry.

PubMed

Wolf, Erich W

2015-08-01

Motion of the spinal cord relative to a spinal cord stimulator epidural electrode array can cause suboptimal stimulation: either noxious, inefficient, or insufficient. Adaptive stimulation attempts to mitigate these effects by modulating stimulation parameters in a position-dependent fashion. Near-infrared (NIR) reflectometry is demonstrated to provide real-time direct measurement of spinal cord position at the site of stimulation, which can facilitate closed-loop adaptive stimulation during static and dynamic motion states. A miniature sensor array consisting of an NIR light emitting diode flanked by phototransistors potted in epoxy was placed in the dorsal epidural space of a human cadaver at the T8 level via laminotomy. Turgor of the subarachnoid space was maintained by intrathecal infusion of saline. NIR reflectance was measured as the cadaver was rotated about its longitudinal axis on a gantry. NIR reflectance was correlated with gantry position and velocity. NIR reflectometry suggests gravitational force is the primary determinant of cord position in static, ordinal positions. Under dynamic motion conditions, there was statistically significant cross-correlation between reflectometry data and the tangential velocity squared, suggesting that centripetal force was the primary determinant of cord position as the gantry was rotated. Reflectometry data strongly correlated with a simple geometric model of anticipated spinal cord precession within the spinal canal. Spinal cord position during dynamic motion has been shown to differ from static predictions due to additional influences such as centripetal force. These findings underscore limitations in extrapolating spinal cord position from surrogates such as body position or body acceleration at sites remote from the stimulating electrodes. NIR reflectometry offers a real-time direct measure of spinal cord position in both static and dynamic motion states, which may facilitate closed-loop adaptive stimulation applications. © 2015 International Neuromodulation Society.

The effects of chronic intracortical microstimulation on neural tissue and fine motor behavior.

PubMed

Rajan, Alexander T; Boback, Jessica L; Dammann, John F; Tenore, Francesco V; Wester, Brock A; Otto, Kevin J; Gaunt, Robert A; Bensmaia, Sliman J

2015-12-01

One approach to conveying sensory feedback in neuroprostheses is to electrically stimulate sensory neurons in the cortex. For this approach to be viable, it is critical that intracortical microstimulation (ICMS) causes minimal damage to the brain. Here, we investigate the effects of chronic ICMS on the neuronal tissue across a variety of stimulation regimes in non-human primates. We also examine each animal's ability to use their hand--the cortical representation of which is targeted by the ICMS--as a further assay of possible neuronal damage. We implanted electrode arrays in the primary somatosensory cortex of three Rhesus macaques and delivered ICMS four hours per day, five days per week, for six months. Multiple regimes of ICMS were delivered to investigate the effects of stimulation parameters on the tissue and behavior. Parameters included current amplitude (10-100 μA), pulse train duration (1, 5 s), and duty cycle (1/1, 1/3). We then performed a range of histopathological assays on tissue near the tips of both stimulated and unstimulated electrodes to assess the effects of chronic ICMS on the tissue and their dependence on stimulation parameters. While the implantation and residence of the arrays in the cortical tissue did cause significant damage, chronic ICMS had no detectable additional effect; furthermore, the animals exhibited no impairments in fine motor control. Chronic ICMS may be a viable means to convey sensory feedback in neuroprostheses as it does not cause significant damage to the stimulated tissue.

Long-term stimulation by active epiretinal implants in normal and RCD1 dogs

NASA Astrophysics Data System (ADS)

Güven, Dilek; Weiland, James D.; Fujii, Gildo; Mech, Brian V.; Mahadevappa, Manjunatha; Greenberg, Robert; Roizenblatt, Roberto; Qiu, Guanting; La Bree, Laurie; Wang, Xiaopeng; Hinton, David; Humayun, Mark S.

2005-03-01

An epiretinal prosthesis, consisting of an extraocular microelectronic stimulator and an intraocular electrode array, was implanted in one eye of three blind and three sighted dogs. Three dogs (2 blind, 1 normal) were stimulated for 120 days, and two dogs (both normal) for 60 and 103 days respectively for 8-10 h/day at levels of 0.1 mC cm-2 and 0.05 mC cm-2, with each stimulus level presented to half of the array. One blind dog was kept as an inactive implant control. During the study period, electroretinograms (ERG) and fundus photographs were recorded. At the end of the study period, the dogs were sacrificed and histological and morphometric evaluation was made of the retina. No inflammatory reaction, neovascularization or hemorrhage was observed during the follow-up examinations. ERGs were unchanged. Stimulus levels used were of sufficient amplitude to elicit cortical evoked potentials. Histological evaluation showed no inflammatory infiltrates or changes in retina morphometry related to electrical stimulation when compared to the unstimulated control eye. Morphometric analysis revealed no consistent differences relating to electrical stimulation. In summary, chronic electrical stimulation of the dog retina at up to 0.1 mC cm-2 with an epiretinal prosthesis does not appear to adversely affect the retina. This study is supported by The Fletcher Jones Foundation, National Eye Institute Grants 1R24EY12893 and EY03040, the Whitaker Foundation and Second Sight Medical Products, Inc.

The effects of chronic intracortical microstimulation on neural tissue and fine motor behavior

NASA Astrophysics Data System (ADS)

Rajan, Alexander T.; Boback, Jessica L.; Dammann, John F.; Tenore, Francesco V.; Wester, Brock A.; Otto, Kevin J.; Gaunt, Robert A.; Bensmaia, Sliman J.

2015-12-01

Objective. One approach to conveying sensory feedback in neuroprostheses is to electrically stimulate sensory neurons in the cortex. For this approach to be viable, it is critical that intracortical microstimulation (ICMS) causes minimal damage to the brain. Here, we investigate the effects of chronic ICMS on the neuronal tissue across a variety of stimulation regimes in non-human primates. We also examine each animal’s ability to use their hand—the cortical representation of which is targeted by the ICMS—as a further assay of possible neuronal damage. Approach. We implanted electrode arrays in the primary somatosensory cortex of three Rhesus macaques and delivered ICMS four hours per day, five days per week, for six months. Multiple regimes of ICMS were delivered to investigate the effects of stimulation parameters on the tissue and behavior. Parameters included current amplitude (10-100 μA), pulse train duration (1, 5 s), and duty cycle (1/1, 1/3). We then performed a range of histopathological assays on tissue near the tips of both stimulated and unstimulated electrodes to assess the effects of chronic ICMS on the tissue and their dependence on stimulation parameters. Main results. While the implantation and residence of the arrays in the cortical tissue did cause significant damage, chronic ICMS had no detectable additional effect; furthermore, the animals exhibited no impairments in fine motor control. Significance. Chronic ICMS may be a viable means to convey sensory feedback in neuroprostheses as it does not cause significant damage to the stimulated tissue.

Time-dependent Increase in the Network Response to the Stimulation of Neuronal Cell Cultures on Micro-electrode Arrays.

PubMed

Gertz, Monica L; Baker, Zachary; Jose, Sharon; Peixoto, Nathalia

2017-05-29

Micro-electrode arrays (MEAs) can be used to investigate drug toxicity, design paradigms for next-generation personalized medicine, and study network dynamics in neuronal cultures. In contrast with more traditional methods, such as patch-clamping, which can only record activity from a single cell, MEAs can record simultaneously from multiple sites in a network, without requiring the arduous task of placing each electrode individually. Moreover, numerous control and stimulation configurations can be easily applied within the same experimental setup, allowing for a broad range of dynamics to be explored. One of the key dynamics of interest in these in vitro studies has been the extent to which cultured networks display properties indicative of learning. Mouse neuronal cells cultured on MEAs display an increase in response following training induced by electrical stimulation. This protocol demonstrates how to culture neuronal cells on MEAs; successfully record from over 95% of the plated dishes; establish a protocol to train the networks to respond to patterns of stimulation; and sort, plot, and interpret the results from such experiments. The use of a proprietary system for stimulating and recording neuronal cultures is demonstrated. Software packages are also used to sort neuronal units. A custom-designed graphical user interface is used to visualize post-stimulus time histograms, inter-burst intervals, and burst duration, as well as to compare the cellular response to stimulation before and after a training protocol. Finally, representative results and future directions of this research effort are discussed.

A microfabricated platform with hydrogel arrays for 3D mechanical stimulation of cells.

PubMed

Liu, Haijiao; Usprech, Jenna; Sun, Yu; Simmons, Craig A

2016-04-01

Cellular microenvironments present cells with multiple stimuli, including not only soluble biochemical and insoluble matrix cues but also mechanical factors. Biomaterial array platforms have been used to combinatorially and efficiently probe and define two-dimensional (2D) and 3D microenvironmental cues to guide cell functions for tissue engineering applications. However, there are few examples of array platforms that include dynamic mechanical forces, particularly to enable stretching of 3D cell-seeded biomaterials, which is relevant to engineering connective and cardiovascular tissues. Here we present a deformable membrane platform that enables 3D dynamic mechanical stretch of arrayed biomaterial constructs. Cell-seeded polyethylene glycol norbornene (PEG-NB) hydrogels were bound to miniaturized deformable membranes via a thiol-ene reaction with off-stoichiometry thiol-ene based polydimethylsiloxane (OSTE-PDMS) as the membrane material. Bonding to OSTE-PDMS enabled the 3D hydrogel microconstructs to be cyclically deformed and stretched by the membrane. As a first demonstration, human mesenchymal stromal cells (MSCs) embedded in PEG-NB were stretched for several days. They were found to be viable, spread in the 3D hydrogels, and exhibited a contractile myofibroblast phenotype when exposed to dynamic 3D mechanical deformation. This platform, which is readily scalable to larger arrays, enables systematic interrogation of the relationships between combinations of 3D mechanobiological cues and cellular responses, and thus has the potential to identify strategies to predictably control the construction of functional engineered tissues. Current high-throughput biomaterial screening approaches fail to consider the effects of dynamic mechanical stimulation, despite its importance in a wide variety of regenerative medicine applications. To meet this need, we developed a deformable membrane platform that enables 3D dynamic stretch of arrayed biomaterial constructs. Our approach combines microtechnologies fabricated with off-stoichiometry thiol-ene based polydimethylsiloxane membranes that can covalently bond cell-seeded polyethylene glycol norbornene 3D hydrogels, a model biomaterial with tunable adhesive, elastic and degradation characteristics. As a first demonstration, we show that human mesenchymal stromal cells embedded in hydrogels and subjected to dynamic mechanical stimulation undergo myofibroblast differentiation. This system is readily scaled up to larger arrays, and will enable systematic and efficient screening of combinations of 3D mechanobiological and biomaterial cues on cell fate and function. Copyright © 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Flexible retinal electrode array

DOEpatents

Okandan, Murat [Albuquerque, NM; Wessendorf, Kurt O [Albuquerque, NM; Christenson, Todd R [Albuquerque, NM

2006-10-24

An electrode array which has applications for neural stimulation and sensing. The electrode array can include a large number of electrodes each of which is flexibly attached to a common substrate using a plurality of springs to allow the electrodes to move independently. The electrode array can be formed from a combination of bulk and surface micromachining, with electrode tips that can include an electroplated metal (e.g. platinum, iridium, gold or titanium) or a metal oxide (e.g. iridium oxide) for biocompatibility. The electrode array can be used to form a part of a neural prosthesis, and is particularly well adapted for use in an implantable retinal prosthesis where the electrodes can be tailored to provide a uniform gentle contact pressure with optional sensing of this contact pressure at one or more of the electrodes.

Performance degradation mechanisms and modes in terrestrial photovoltaic arrays and technology for their diagnosis

NASA Technical Reports Server (NTRS)

Noel, G. T.; Sliemers, F. A.; Derringer, G. C.; Wood, V. E.; Wilkes, K. E.; Gaines, G. B.; Carmichael, D. C.

1978-01-01

Accelerated life-prediction test methodologies have been developed for the validation of a 20-year service life for low-cost photovoltaic arrays. Array failure modes, relevant materials property changes, and primary degradation mechanisms are discussed as a prerequisite to identifying suitable measurement techniques and instruments. Measurements must provide sufficient confidence to permit selection among alternative designs and materials and to stimulate widespread deployment of such arrays. Furthermore, the diversity of candidate materials and designs, and the variety of potential environmental stress combinations, degradation mechanisms and failure modes require that combinations of measurement techniques be identified which are suitable for the characterization of various encapsulation system-cell structure-environment combinations.

Visual and Vestibular Determinants of Perceived Eye-Level

NASA Technical Reports Server (NTRS)

Cohen, Malcolm Martin

2003-01-01

Both gravitational and optical sources of stimulation combine to determine the perceived elevations of visual targets. The ways in which these sources of stimulation combine with one another in operational aeronautical environments are critical for pilots to make accurate judgments of the relative altitudes of other aircraft and of their own altitude relative to the terrain. In a recent study, my colleagues and I required eighteen observers to set visual targets at their apparent horizon while they experienced various levels of G(sub z) in the human centrifuge at NASA-Ames Research Center. The targets were viewed in darkness and also against specific background optical arrays that were oriented at various angles with respect to the vertical; target settings were lowered as Gz was increased; this effect was reduced when the background optical array was visible. Also, target settings were displaced in the direction that the background optical array was pitched. Our results were attributed to the combined influences of otolith-oculomotor mechanisms that underlie the elevator illusion and visual-oculomotor mechanisms (optostatic responses) that underlie the perceptual effects of viewing pitched optical arrays that comprise the background. In this paper, I present a mathematical model that describes the independent and combined effects of G(sub z) intensity and the orientation and structure of background optical arrays; the model predicts quantitative deviations from normal accurate perceptions of target localization under a variety of conditions. Our earlier experimental results and the mathematical model are described in some detail, and the effects of viewing specific optical arrays under various gravitational-inertial conditions encountered in aeronautical environments are discussed.

Regulation of gene expression in autoimmune disease loci and the genetic basis of proliferation in CD4+ effector memory T cells.

PubMed

Hu, Xinli; Kim, Hyun; Raj, Towfique; Brennan, Patrick J; Trynka, Gosia; Teslovich, Nikola; Slowikowski, Kamil; Chen, Wei-Min; Onengut, Suna; Baecher-Allan, Clare; De Jager, Philip L; Rich, Stephen S; Stranger, Barbara E; Brenner, Michael B; Raychaudhuri, Soumya

2014-06-01

Genome-wide association studies (GWAS) and subsequent dense-genotyping of associated loci identified over a hundred single-nucleotide polymorphism (SNP) variants associated with the risk of rheumatoid arthritis (RA), type 1 diabetes (T1D), and celiac disease (CeD). Immunological and genetic studies suggest a role for CD4-positive effector memory T (CD+ TEM) cells in the pathogenesis of these diseases. To elucidate mechanisms of autoimmune disease alleles, we investigated molecular phenotypes in CD4+ effector memory T cells potentially affected by these variants. In a cohort of genotyped healthy individuals, we isolated high purity CD4+ TEM cells from peripheral blood, then assayed relative abundance, proliferation upon T cell receptor (TCR) stimulation, and the transcription of 215 genes within disease loci before and after stimulation. We identified 46 genes regulated by cis-acting expression quantitative trait loci (eQTL), the majority of which we detected in stimulated cells. Eleven of the 46 genes with eQTLs were previously undetected in peripheral blood mononuclear cells. Of 96 risk alleles of RA, T1D, and/or CeD in densely genotyped loci, eleven overlapped cis-eQTLs, of which five alleles completely explained the respective signals. A non-coding variant, rs389862A, increased proliferative response (p=4.75 × 10-8). In addition, baseline expression of seventeen genes in resting cells reliably predicted proliferative response after TCR stimulation. Strikingly, however, there was no evidence that risk alleles modulated CD4+ TEM abundance or proliferation. Our study underscores the power of examining molecular phenotypes in relevant cells and conditions for understanding pathogenic mechanisms of disease variants.

Efficient Usage of Dense GNSS Networks in Central Europe for the Visualization and Investigation of Ionospheric TEC Variations

PubMed Central

Zanimonskiy, Yevgen M.; Yampolski, Yuri M.; Figurski, Mariusz

2017-01-01

The technique of the orthogonal projection of ionosphere electronic content variations for mapping total electron content (TEC) allows us to visualize ionospheric irregularities. For the reconstruction of global ionospheric characteristics, numerous global navigation satellite system (GNSS) receivers located in different regions of the Earth are used as sensors. We used dense GNSS networks in central Europe to detect and investigate a special type of plasma inhomogeneities, called travelling ionospheric disturbances (TID). Such use of GNSS sensors allows us to reconstruct the main TID parameters, such as spatial dimensions, velocities, and directions of their movement. The paper gives examples of the restoration of dynamic characteristics of ionospheric irregularities for quiet and disturbed geophysical conditions. Special attention is paid to the dynamics of ionospheric disturbances stimulated by the magnetic storms of two St. Patrick’s Days (17 March 2013 and 2015). Additional opportunities for the remote sensing of the ionosphere with the use of dense regional networks of GNSS receiving sensors have been noted too. PMID:28994718

Efficient Usage of Dense GNSS Networks in Central Europe for the Visualization and Investigation of Ionospheric TEC Variations.

PubMed

Nykiel, Grzegorz; Zanimonskiy, Yevgen M; Yampolski, Yuri M; Figurski, Mariusz

2017-10-10

The technique of the orthogonal projection of ionosphere electronic content variations for mapping total electron content (TEC) allows us to visualize ionospheric irregularities. For the reconstruction of global ionospheric characteristics, numerous global navigation satellite system (GNSS) receivers located in different regions of the Earth are used as sensors. We used dense GNSS networks in central Europe to detect and investigate a special type of plasma inhomogeneities, called travelling ionospheric disturbances (TID). Such use of GNSS sensors allows us to reconstruct the main TID parameters, such as spatial dimensions, velocities, and directions of their movement. The paper gives examples of the restoration of dynamic characteristics of ionospheric irregularities for quiet and disturbed geophysical conditions. Special attention is paid to the dynamics of ionospheric disturbances stimulated by the magnetic storms of two St. Patrick's Days (17 March 2013 and 2015). Additional opportunities for the remote sensing of the ionosphere with the use of dense regional networks of GNSS receiving sensors have been noted too.

Wafer-scale, massively parallel carbon nanotube arrays for realizing field effect transistors with current density exceeding silicon and gallium arsenide

NASA Astrophysics Data System (ADS)

Arnold, Michael

Calculations have indicated that aligned arrays of semiconducting carbon nanotubes (CNTs) promise to outperform conventional semiconducting materials in short-channel, aggressively scaled field effect transistors (FETs) like those used in semiconductor logic and high frequency amplifier technologies. These calculations have been based on extrapolation of measurements of FETs based on one CNT, in which ballistic transport approaching the quantum conductance limit of 2Go = 4e2/h has been achieved. However, constraints in CNT sorting, processing, alignment, and contacts give rise to non-idealities when CNTs are implemented in densely-packed parallel arrays, which has resulted in a conductance per CNT far from 2Go. The consequence has been that it has been very difficult to create high performance CNT array FETs, and CNT array FETs have not outperformed but rather underperformed channel materials such as Si by 6 x or more. Here, we report nearly ballistic CNT array FETs at a density of 50 CNTs um-1, created via CNT sorting, wafer-scale alignment and assembly, and treatment. The on-state conductance in the arrays is as high as 0.46 Go per CNT, and the conductance of the arrays reaches 1.7 mS um-1, which is 7 x higher than previous state-of-the-art CNT array FETs made by other methods. The saturated on-state current density reaches 900 uA um-1 and is similar to or exceeds that of Si FETs when compared at equivalent gate oxide thickness, off-state current density, and channel length. The on-state current density exceeds that of GaAs FETs, as well. This leap in CNT FET array performance is a significant advance towards the exploitation of CNTs in high-performance semiconductor electronics technologies.

One to Large N Gradiometry

NASA Astrophysics Data System (ADS)

Langston, C. A.

2017-12-01

The seismic wave gradient tensor can be derived from a variety of field observations including measurements of the wavefield by a dense seismic array, strain meters, and rotation meters. Coupled with models of wave propagation, wave gradients along with the original wavefield can give estimates of wave attributes that can be used to infer wave propagation directions, apparent velocities, spatial amplitude behavior, and wave type. Compact geodetic arrays with apertures of 0.1 wavelength or less can be deployed to provide wavefield information at a localized spot similar to larger phased arrays with apertures of many wavelengths. Large N, spatially distributed arrays can provide detailed information over an area to detect structure changes. Key to accurate computation of spatial gradients from arrays of seismic instruments is knowledge of relative instrument responses, particularly component sensitivities and gains, along with relative sensor orientations. Array calibration has been successfully performed for the 14-element Pinyon Flat, California, broadband array using long-period teleseisms to achieve relative precisions as small as 0.2% in amplitude and 0.35o in orientation. Calibration has allowed successful comparison of horizontal seismic strains from local and regional seismic events with the Plate Boundary Observatory (PBO) borehole strainmeter located at the facility. Strains from the borehole strainmeter in conjunction with ground velocity from a co-located seismometer are used as a "point" array in estimating wave attributes for the P-SV components of the wavefield. An effort is underway to verify the calibration of PBO strainmeters in southern California and their co-located borehole seismic sensors to create an array of point arrays for use in studies of regional wave propagation and seismic sources.

Protein retention on plasma-treated hierarchical nanoscale gold-silver platform

PubMed Central

Fang, Jinghua; Levchenko, Igor; Mai-Prochnow, Anne; Keidar, Michael; Cvelbar, Uros; Filipic, Gregor; Han, Zhao Jun; Ostrikov, Kostya (Ken)

2015-01-01

Dense arrays of gold-supported silver nanowires of about 100 nm in diameter grown directly in the channels of nanoporous aluminium oxide membrane were fabricated and tested as a novel platform for the immobilization and retention of BSA proteins in the microbial-protective environments. Additional treatment of the silver nanowires using low-temperature plasmas in the inductively-coupled plasma reactor and an atmospheric-pressure plasma jet have demonstrated that the morphology of the nanowire array can be controlled and the amount of the retained protein may be increased due to the plasma effect. A combination of the neutral gold sublayer with the antimicrobial properties of silver nanowires could significantly enhance the efficiency of the platforms used in various biotechnological processes. PMID:26307515

Deep turbulence effects mitigation with coherent combining of 21 laser beams over 7 km.

PubMed

Weyrauch, Thomas; Vorontsov, Mikhail; Mangano, Joseph; Ovchinnikov, Vladimir; Bricker, David; Polnau, Ernst; Rostov, Andrey

2016-02-15

We demonstrate coherent beam combining and adaptive mitigation of atmospheric turbulence effects over 7 km under strong scintillation conditions using a coherent fiber array laser transmitter operating in a target-in-the-loop setting. The transmitter system is composed of a densely packed array of 21 fiber collimators with integrated capabilities for piston, tip, and tilt control of the outgoing beams wavefront phases. A small cat's-eye retro reflector was used for evaluation of beam combining and turbulence compensation performance at the target plane, and to provide the feedback signal for control of piston and tip/tilt phases of the transmitted beams using the stochastic parallel gradient descent maximization of the power-in-the-bucket metric.

A neurophysiological explanation for biases in visual localization.

PubMed

Moreland, James C; Boynton, Geoffrey M

2017-02-01

Observers show small but systematic deviations from equal weighting of all elements when asked to localize the center of an array of dots. Counter-intuitively, with small numbers of dots drawn from a Gaussian distribution, this bias results in subjects overweighting the influence of outlier dots - inconsistent with traditional statistical estimators of central tendency. Here we show that this apparent statistical anomaly can be explained by the observation that outlier dots also lie in regions of lower dot density. Using a standard model of V1 processing, which includes spatial integration followed by a compressive static nonlinearity, we can successfully predict the finding that dots in less dense regions of an array have a relatively greater influence on the perceived center.

Protein retention on plasma-treated hierarchical nanoscale gold-silver platform

NASA Astrophysics Data System (ADS)

Fang, Jinghua; Levchenko, Igor; Mai-Prochnow, Anne; Keidar, Michael; Cvelbar, Uros; Filipic, Gregor; Han, Zhao Jun; Ostrikov, Kostya (Ken)

2015-08-01

Dense arrays of gold-supported silver nanowires of about 100 nm in diameter grown directly in the channels of nanoporous aluminium oxide membrane were fabricated and tested as a novel platform for the immobilization and retention of BSA proteins in the microbial-protective environments. Additional treatment of the silver nanowires using low-temperature plasmas in the inductively-coupled plasma reactor and an atmospheric-pressure plasma jet have demonstrated that the morphology of the nanowire array can be controlled and the amount of the retained protein may be increased due to the plasma effect. A combination of the neutral gold sublayer with the antimicrobial properties of silver nanowires could significantly enhance the efficiency of the platforms used in various biotechnological processes.

Ipsilateral masking between acoustic and electric stimulations.

PubMed

Lin, Payton; Turner, Christopher W; Gantz, Bruce J; Djalilian, Hamid R; Zeng, Fan-Gang

2011-08-01

Residual acoustic hearing can be preserved in the same ear following cochlear implantation with minimally traumatic surgical techniques and short-electrode arrays. The combined electric-acoustic stimulation significantly improves cochlear implant performance, particularly speech recognition in noise. The present study measures simultaneous masking by electric pulses on acoustic pure tones, or vice versa, to investigate electric-acoustic interactions and their underlying psychophysical mechanisms. Six subjects, with acoustic hearing preserved at low frequencies in their implanted ear, participated in the study. One subject had a fully inserted 24 mm Nucleus Freedom array and five subjects had Iowa/Nucleus hybrid implants that were only 10 mm in length. Electric masking data of the long-electrode subject showed that stimulation from the most apical electrodes produced threshold elevations over 10 dB for 500, 625, and 750 Hz probe tones, but no elevation for 125 and 250 Hz tones. On the contrary, electric stimulation did not produce any electric masking in the short-electrode subjects. In the acoustic masking experiment, 125-750 Hz pure tones were used to acoustically mask electric stimulation. The acoustic masking results showed that, independent of pure tone frequency, both long- and short-electrode subjects showed threshold elevations at apical and basal electrodes. The present results can be interpreted in terms of underlying physiological mechanisms related to either place-dependent peripheral masking or place-independent central masking.

Direct Intracochlear Acoustic Stimulation Using a PZT Microactuator.

PubMed

Luo, Chuan; Omelchenko, Irina; Manson, Robert; Robbins, Carol; Oesterle, Elizabeth C; Cao, Guo Zhong; Shen, I Y; Hume, Clifford R

2015-12-01

Combined electric and acoustic stimulation has proven to be an effective strategy to improve hearing in some cochlear implant users. We describe an acoustic microactuator to directly deliver stimuli to the perilymph in the scala tympani. The 800 µm by 800 µm actuator has a silicon diaphragm driven by a piezoelectric thin film (e.g., lead-zirconium-titanium oxide or PZT). This device could also be used as a component of a bimodal acoustic-electric electrode array. In the current study, we established a guinea pig model to test the actuator for its ability to deliver auditory signals to the cochlea in vivo. The actuator was placed through the round window of the cochlea. Auditory brainstem response (ABR) thresholds, peak latencies, and amplitude growth were calculated for an ear canal speaker versus the intracochlear actuator for tone burst stimuli at 4, 8, 16, and 24 kHz. An ABR was obtained after removal of the probe to assess loss of hearing related to the procedure. In some animals, the temporal bone was harvested for histologic analysis of cochlear damage. We show that the device is capable of stimulating ABRs in vivo with latencies and growth functions comparable to stimulation in the ear canal. Further experiments will be necessary to evaluate the efficiency and safety of this modality in long-term auditory stimulation and its ability to be integrated with conventional cochlear implant arrays. © The Author(s) 2015.

Design of a high-resolution optoelectronic retinal prosthesis.

PubMed

Palanker, Daniel; Vankov, Alexander; Huie, Phil; Baccus, Stephen

2005-03-01

It has been demonstrated that electrical stimulation of the retina can produce visual percepts in blind patients suffering from macular degeneration and retinitis pigmentosa. However, current retinal implants provide very low resolution (just a few electrodes), whereas at least several thousand pixels would be required for functional restoration of sight. This paper presents the design of an optoelectronic retinal prosthetic system with a stimulating pixel density of up to 2500 pix mm(-2) (corresponding geometrically to a maximum visual acuity of 20/80). Requirements on proximity of neural cells to the stimulation electrodes are described as a function of the desired resolution. Two basic geometries of sub-retinal implants providing required proximity are presented: perforated membranes and protruding electrode arrays. To provide for natural eye scanning of the scene, rather than scanning with a head-mounted camera, the system operates similar to 'virtual reality' devices. An image from a video camera is projected by a goggle-mounted collimated infrared LED-LCD display onto the retina, activating an array of powered photodiodes in the retinal implant. The goggles are transparent to visible light, thus allowing for the simultaneous use of remaining natural vision along with prosthetic stimulation. Optical delivery of visual information to the implant allows for real-time image processing adjustable to retinal architecture, as well as flexible control of image processing algorithms and stimulation parameters.

A wideband wireless neural stimulation platform for high-density microelectrode arrays.

PubMed

Myers, Frank B; Simpson, Jim A; Ghovanloo, Maysam

2006-01-01

We describe a system that allows researchers to control an implantable neural microstimulator from a PC via a USB 2.0 interface and a novel dual-carrier wireless link, which provides separate data and power transmission. Our wireless stimulator, Interestim-2B (IS-2B), is a modular device capable of generating controlled-current stimulation pulse trains across 32 sites per module with support for a variety of stimulation schemes (biphasic/monophasic, bipolar/monopolar). We have developed software to generate multi-site stimulation commands for the IS-2B based on streaming data from artificial sensory devices such as cameras and microphones. For PC interfacing, we have developed a USB 2.0 microcontroller-based interface. Data is transmitted using frequency-shift keying (FSK) at 6/12 MHz to achieve a data rate of 3 Mb/s via a pair of rectangular coils. Power is generated using a class-E power amplifier operating at 1 MHz and transmitted via a separate pair of spiral planar coils which are oriented perpendicular to the data coils to minimize cross-coupling. We have successfully demonstrated the operation of the system by applying it as a visual prosthesis. Pulse-frequency modulated stimuli are generated in real-time based on a grayscale image from a webcam. These pulses are projected onto an 11x11 LED matrix that represents a 2D microelectrode array.

Design of a high-resolution optoelectronic retinal prosthesis

NASA Astrophysics Data System (ADS)

Palanker, Daniel; Vankov, Alexander; Huie, Phil; Baccus, Stephen

2005-03-01

It has been demonstrated that electrical stimulation of the retina can produce visual percepts in blind patients suffering from macular degeneration and retinitis pigmentosa. However, current retinal implants provide very low resolution (just a few electrodes), whereas at least several thousand pixels would be required for functional restoration of sight. This paper presents the design of an optoelectronic retinal prosthetic system with a stimulating pixel density of up to 2500 pix mm-2 (corresponding geometrically to a maximum visual acuity of 20/80). Requirements on proximity of neural cells to the stimulation electrodes are described as a function of the desired resolution. Two basic geometries of sub-retinal implants providing required proximity are presented: perforated membranes and protruding electrode arrays. To provide for natural eye scanning of the scene, rather than scanning with a head-mounted camera, the system operates similar to 'virtual reality' devices. An image from a video camera is projected by a goggle-mounted collimated infrared LED-LCD display onto the retina, activating an array of powered photodiodes in the retinal implant. The goggles are transparent to visible light, thus allowing for the simultaneous use of remaining natural vision along with prosthetic stimulation. Optical delivery of visual information to the implant allows for real-time image processing adjustable to retinal architecture, as well as flexible control of image processing algorithms and stimulation parameters.

Carbon fiber on polyimide ultra-microelectrodes

NASA Astrophysics Data System (ADS)

Gillis, Winthrop F.; Lissandrello, Charles A.; Shen, Jun; Pearre, Ben W.; Mertiri, Alket; Deku, Felix; Cogan, Stuart; Holinski, Bradley J.; Chew, Daniel J.; White, Alice E.; Otchy, Timothy M.; Gardner, Timothy J.

2018-02-01

Objective. Most preparations for making neural recordings degrade over time and eventually fail due to insertion trauma and reactive tissue response. The magnitudes of these responses are thought to be related to the electrode size (specifically, the cross-sectional area), the relative stiffness of the electrode, and the degree of tissue tolerance for the material. Flexible carbon fiber ultra-microelectrodes have a much smaller cross-section than traditional electrodes and low tissue reactivity, and thus may enable improved longevity of neural recordings in the central and peripheral nervous systems. Only two carbon fiber array designs have been described previously, each with limited channel densities due to limitations of the fabrication processes or interconnect strategies. Here, we describe a method for assembling carbon fiber electrodes on a flexible polyimide substrate that is expected to facilitate the construction of high-density recording and stimulating arrays. Approach. Individual carbon fibers were aligned using an alignment tool that was 3D-printed with sub-micron resolution using direct laser writing. Indium deposition on the carbon fibers, followed by low-temperature microsoldering, provided a robust and reliable method of electrical connection to the polyimide interconnect. Main results. Spontaneous multiunit activity and stimulation-evoked compound responses with SNR  >10 and  >120, respectively, were recorded from a small (125 µm) peripheral nerve. We also improved the typically poor charge injection capacity of small diameter carbon fibers by electrodepositing 100 nm-thick iridium oxide films, making the carbon fiber arrays usable for electrical stimulation as well as recording. Significance. Our innovations in fabrication technique pave the way for further miniaturization of carbon fiber ultra-microelectrode arrays. We believe these advances to be key steps to enable a shift from labor intensive, manual assembly to a more automated manufacturing process.

Carbon fiber on polyimide ultra-microelectrodes.

PubMed

Gillis, Winthrop F; Lissandrello, Charles A; Shen, Jun; Pearre, Ben W; Mertiri, Alket; Deku, Felix; Cogan, Stuart; Holinski, Bradley J; Chew, Daniel J; White, Alice E; Otchy, Timothy M; Gardner, Timothy J

2018-02-01

Most preparations for making neural recordings degrade over time and eventually fail due to insertion trauma and reactive tissue response. The magnitudes of these responses are thought to be related to the electrode size (specifically, the cross-sectional area), the relative stiffness of the electrode, and the degree of tissue tolerance for the material. Flexible carbon fiber ultra-microelectrodes have a much smaller cross-section than traditional electrodes and low tissue reactivity, and thus may enable improved longevity of neural recordings in the central and peripheral nervous systems. Only two carbon fiber array designs have been described previously, each with limited channel densities due to limitations of the fabrication processes or interconnect strategies. Here, we describe a method for assembling carbon fiber electrodes on a flexible polyimide substrate that is expected to facilitate the construction of high-density recording and stimulating arrays. Individual carbon fibers were aligned using an alignment tool that was 3D-printed with sub-micron resolution using direct laser writing. Indium deposition on the carbon fibers, followed by low-temperature microsoldering, provided a robust and reliable method of electrical connection to the polyimide interconnect. Spontaneous multiunit activity and stimulation-evoked compound responses with SNR  >10 and  >120, respectively, were recorded from a small (125 µm) peripheral nerve. We also improved the typically poor charge injection capacity of small diameter carbon fibers by electrodepositing 100 nm-thick iridium oxide films, making the carbon fiber arrays usable for electrical stimulation as well as recording. Our innovations in fabrication technique pave the way for further miniaturization of carbon fiber ultra-microelectrode arrays. We believe these advances to be key steps to enable a shift from labor intensive, manual assembly to a more automated manufacturing process.

Crustal structure of the Izu Collision zone, central Japan, revealed by dense seismic array observations

NASA Astrophysics Data System (ADS)

Kurashimo, E.; Sato, H.; Abe, S.; Kato, N.; Ishikawa, M.; Obara, K.

2009-12-01

In central Japan, the Philippine Sea Plate (PSP) subducts beneath the Tokyo Metropolitan area, the Kanto region. In western Kanto region, the Izu-Bonin arc (IBA) within the PSP has been colliding from the south with the Honshu arc, forming a complex structure called the Izu-Collision zone (ICZ). Several active faults were formed in and around the ICZ. The geometry of the subducting PSP and the overlying crustal structure of the ICZ are important to constrain the process of earthquake occurrence and the crustal evolution process associated with arc-arc collision. Recent seismic experiments reveal the geometry of the subducting PSP beneath the Kanto region (Sato et al., 2005). The Japanese islands, including the ICZ, are covered with dense arrays of permanent seismic stations, which provide good constraints on velocity structures by a tomographic method. Such studies reveal a general picture of the lithospheric structure such as a descending plate configuration (e.g. Matsubara et al., 2008). However, since an average spacing of the permanent station is typically 20 km, a detailed structure in the upper crust, which is imperative for an understanding of the active tectonics, cannot be well constrained by permanent array alone. Two dense seismic array observations were conducted to obtain a structural image beneath the ICZ. One is a 40-km-long line (EW-line) located in the northern part of the ICZ and the other is a 55-km-long line (NS-line) located in the central part of the ICZ. Seventy-five 3-component portable seismographs were deployed on EW-line with 500 to 700 m interval and waveforms were recorded during a four-month period from October, 2008. Forty 3-component portable seismographs were deployed on NS-line with about 1 km spacing and waveforms were recorded during the three month period from January, 2006. In order to obtain a high-resolution velocity model, a well-controlled hypocenter is essential. Due to this, we combined the seismic array data with permanent seismic station data. P- and S-wave arrival time data were obtained from 247 events and 16,144 P- and 13,723 S-wave arrival times were used for the inversion analysis. Arrival times of local earthquakes were used in a joint inversion for earthquake locations and 3-D Vp and Vp/Vs structures, using the iterative damped least-squares algorithm, simul2000 (Thurber and Eberhart-Phillips, 1999). The P-wave velocity structure shows that low velocity zones exist along the estimated deeper extension of the active faults and high velocity zones exist beneath the Tanzawa Mountains and Misaka Mountains. The Tanzawa Mountains and the Misaka Mountains are considered as fragments of the IBA (e.g. Niitsuma, 1989). We obtained a seismic velocity model revealing good correlations with the surface geology along the profile. Acknowledgments: This study was supported by the Earthquake Research Institute cooperative research program.

Does cochlear implantation and electrical stimulation affect residual hair cells and spiral ganglion neurons?

PubMed Central

Coco, Anne; Epp, Stephanie B.; Fallon, James B.; Xu, Jin; Millard, Rodney E.; Shepherd, Robert K.

2007-01-01

Increasing numbers of cochlear implant subjects have some level of residual hearing at the time of implantation. The present study examined whether (i) hair cells that have survived one pathological insult (aminoglycoside deafening), can survive and function following long-term cochlear implantation and electrical stimulation (ES); and (ii) chronic ES in these cochleae results in greater trophic support of spiral ganglion neurons (SGNs) compared with cochleae devoid of hair cells. Eight cats, with either partial (n=4) or severe (n=4) sensorineural hearing loss, were bilaterally implanted with scala tympani electrode arrays 2 months after deafening, and received unilateral ES using charge balanced biphasic current pulses for periods of up to 235 days. Frequency-specific compound action potentials and click-evoked auditory brainstem responses (ABRs) were recorded periodically to monitor the residual acoustic hearing. Electrically-evoked ABRs (EABRs) were recorded to confirm the stimulus levels were 3-6 dB above the EABR threshold. On completion of the ES program the cochleae were examined histologically. Partially deafened animals showed no significant increase in acoustic thresholds over the implantation period. Moreover, chronic ES of an electrode array located in the base of the cochlea did not adversely affect hair cells in the middle or apical turns. There was evidence of a small but statistically significant rescue of SGNs in the middle and apical turns of stimulated cochleae in animals with partial hearing. Chronic ES did not, however, prevent a reduction in SGN density for the severely deaf cohort, although SGNs adjacent to the stimulating electrodes did exhibit a significant increase in soma area (p<0.01). In sum, chronic ES in partial hearing animals does not adversely affect functioning residual hair cells apical to the electrode array. Moreover, while there is an increase in the soma area of SGNs close to the stimulating electrodes in severely deaf cochleae, this trophic effect does not result in increased SGN survival. PMID:17258411

Fabrication and optically pumped lasing of plasmonic nanolaser with regular ZnO/GaN nanoheterojunction array

NASA Astrophysics Data System (ADS)

Huang, Xiaoping; Zhang, Peifeng; Lin, En; Wang, Peng; Mei, Mingwei; Huang, Qiuying; Jiao, Jiao; Zhao, Qing

2017-09-01

We present the design and fabrication of a novel regularly arrayed plasmonic nanolasers. This main microstructure of the device is composed of a hexagonal array of n-ZnO/p-GaN nanoheterojunctions fabricated using the micro-fabrication method. Furthermore, the optically pumped lasing in the device is demonstrated. The spectroscopy characterization results of the device show that the surface plasmon excited around the NWs surface can be used to stimulate and strongly compress the optical modes in the NW cavity. This electromagnetic confinement effect is employed to optimize the beam quality and increase the light intensity compared to the laser fabricated with the bare NWs array. The impact of the array arrangement on the coherent combining efficiency of the arrayed nanolasers has been numerically studied. The results show that the arrayed hexagonal nanolasers could improve the combining efficiency compared to the nanolaser with the randomly positioned array. Qualitatively, these calculated results agree well with the experimental results of the laser beam spot mapping. This demonstrates the scope for using such architectures to improve the combination efficiency of the arrayed nanolasers.

IRIS Arrays: Observing Wavefields at Multiple Scales and Frequencies

NASA Astrophysics Data System (ADS)

Sumy, D. F.; Woodward, R.; Frassetto, A.

2014-12-01

The Incorporated Research Institutions for Seismology (IRIS) provides instruments for creating and operating seismic arrays at a wide range of scales. As an example, for over thirty years the IRIS PASSCAL program has provided instruments to individual Principal Investigators to deploy arrays of all shapes and sizes on every continent. These arrays have ranged from just a few sensors to hundreds or even thousands of sensors, covering areas with dimensions of meters to thousands of kilometers. IRIS also operates arrays directly, such as the USArray Transportable Array (TA) as part of the EarthScope program. Since 2004, the TA has rolled across North America, at any given time spanning a swath of approximately 800 km by 2,500 km, and thus far sampling 2% of the Earth's surface. This achievement includes all of the lower-48 U.S., southernmost Canada, and now parts of Alaska. IRIS has also facilitated specialized arrays in polar environments and on the seafloor. In all cases, the data from these arrays are freely available to the scientific community. As the community of scientists who use IRIS facilities and data look to the future they have identified a clear need for new array capabilities. In particular, as part of its Wavefields Initiative, IRIS is exploring new technologies that can enable large, dense array deployments to record unaliased wavefields at a wide range of frequencies. Large-scale arrays might utilize multiple sensor technologies to best achieve observing objectives and optimize equipment and logistical costs. Improvements in packaging and power systems can provide equipment with reduced size, weight, and power that will reduce logistical constraints for large experiments, and can make a critical difference for deployments in harsh environments or other situations where rapid deployment is required. We will review the range of existing IRIS array capabilities with an overview of previous and current deployments and examples of data and results. We will review existing IRIS projects that explore new array capabilities and highlight future directions for IRIS instrumentation facilities.

Morphology modulation of SrTiO3/TiO2 heterostructures for enhanced photoelectrochemical performance.

PubMed

Jiao, Zhengbo; Chen, Tao; Yu, Hongchao; Wang, Teng; Lu, Gongxuan; Bi, Yingpu

2014-04-01

Design and fabrication of nanoscale semiconductors with regulatable morphology or structure has attracted tremendous interest due to the dependency relationship between properties and architectures. Two types of SrTiO3/TiO2 nanocomposites with different morphologies and structures have been fabricated by controlling the kinetics of hydrothermal reactions. One is TiO2 nanotube arrays densely wrapped by SrTiO3 film and the other is SrTiO3 nanospheres distributed on the top region of TiO2 nanotube arrays, which has been firstly fabricated. It has been found that the photoelectrochemical performances of these heterostructures are crucially dominated by their architectures. Heterostructured SrTiO3/TiO2 nanotube arrays were fabricated by traditional method in the absence of NaOH and they exhibited higher photoelectrochemical performance than pure TiO2 nanotube arrays. However, the compact SrTiO3 coating film on the sidewalls of TiO2 nanotube arrays could inevitably destroy the tubular structures of TiO2 and thus go against the vectorial transport of electrons. Interestingly, when excess NaOH was added into the growth solution, SrTiO3 nanospheres would be rationally grafted on the top of TiO2 nanotube arrays, which could preserve the tubular structures of TiO2, and thus further improve the photoelectrochemical performance. Copyright © 2013 Elsevier Inc. All rights reserved.

Ge quantum dot arrays grown by ultrahigh vacuum molecular-beam epitaxy on the Si(001) surface: nucleation, morphology, and CMOS compatibility.

PubMed

Yuryev, Vladimir A; Arapkina, Larisa V

2011-09-05

Issues of morphology, nucleation, and growth of Ge cluster arrays deposited by ultrahigh vacuum molecular beam epitaxy on the Si(001) surface are considered. Difference in nucleation of quantum dots during Ge deposition at low (≲600°C) and high (≳600°C) temperatures is studied by high resolution scanning tunneling microscopy. The atomic models of growth of both species of Ge huts--pyramids and wedges-- are proposed. The growth cycle of Ge QD arrays at low temperatures is explored. A problem of lowering of the array formation temperature is discussed with the focus on CMOS compatibility of the entire process; a special attention is paid upon approaches to reduction of treatment temperature during the Si(001) surface pre-growth cleaning, which is at once a key and the highest-temperature phase of the Ge/Si(001) quantum dot dense array formation process. The temperature of the Si clean surface preparation, the final high-temperature step of which is, as a rule, carried out directly in the MBE chamber just before the structure deposition, determines the compatibility of formation process of Ge-QD-array based devices with the CMOS manufacturing cycle. Silicon surface hydrogenation at the final stage of its wet chemical etching during the preliminary cleaning is proposed as a possible way of efficient reduction of the Si wafer pre-growth annealing temperature.

Development of an Ultrasonic Phased Array System for Wellbore Integrity Evaluation and Near-Wellbore Fracture Network Mapping of Injection and Production Wells in Geothermal Energy Systems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Almansouri, Hani; Foster, Benjamin; Kisner, Roger A

2016-01-01

This paper documents our progress developing an ultrasound phased array system in combination with a model-based iterative reconstruction (MBIR) algorithm to inspect the health of and characterize the composition of the near-wellbore region for geothermal reservoirs. The main goal for this system is to provide a near-wellbore in-situ characterization capability that will significantly improve wellbore integrity evaluation and near well-bore fracture network mapping. A more detailed image of the fracture network near the wellbore in particular will enable the selection of optimal locations for stimulation along the wellbore, provide critical data that can be used to improve stimulation design, andmore » provide a means for measuring evolution of the fracture network to support long term management of reservoir operations. Development of such a measurement capability supports current hydrothermal operations as well as the successful demonstration of Engineered Geothermal Systems (EGS). The paper will include the design of the phased array system, the performance specifications, and characterization methodology. In addition, we will describe the MBIR forward model derived for the phased array system and the propagation of compressional waves through a pseudo-homogenous medium.« less

Thermal Conduction in Vertically Aligned Copper Nanowire Arrays and Composites.

PubMed

Barako, Michael T; Roy-Panzer, Shilpi; English, Timothy S; Kodama, Takashi; Asheghi, Mehdi; Kenny, Thomas W; Goodson, Kenneth E

2015-09-02

The ability to efficiently and reliably transfer heat between sources and sinks is often a bottleneck in the thermal management of modern energy conversion technologies ranging from microelectronics to thermoelectric power generation. These interfaces contribute parasitic thermal resistances that reduce device performance and are subjected to thermomechanical stresses that degrade device lifetime. Dense arrays of vertically aligned metal nanowires (NWs) offer the unique combination of thermal conductance from the constituent metal and mechanical compliance from the high aspect ratio geometry to increase interfacial heat transfer and device reliability. In the present work, we synthesize copper NW arrays directly onto substrates via templated electrodeposition and extend this technique through the use of a sacrificial overplating layer to achieve improved uniformity. Furthermore, we infiltrate the array with an organic phase change material and demonstrate the preservation of thermal properties. We use the 3ω method to measure the axial thermal conductivity of freestanding copper NW arrays to be as high as 70 W m(-1) K(-1), which is more than an order of magnitude larger than most commercial interface materials and enhanced-conductivity nanocomposites reported in the literature. These arrays are highly anisotropic, and the lateral thermal conductivity is found to be only 1-2 W m(-1) K(-1). We use these measured properties to elucidate the governing array-scale transport mechanisms, which include the effects of morphology and energy carrier scattering from size effects and grain boundaries.

Fast fabrication of long TiO2 nanotube array with high photoelectrochemical property on flexible stainless steel.

PubMed

Tao, Jie; Wu, Tao; Gao, Peng

2012-03-01

Oriented highly ordered long TiO2 nanotube array films with nanopore structure and high photoelectrochemical property were fabricated on flexible stainless steel substrate (50 microm) by anodization treatment of titanium thin films in a short time. The samples were characterized by means of field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD) and photoelectrochemical methods, respectively. The results showed that Ti films deposited at the condition of 0.7 Pa Ar pressure and 96 W sputtering power at room temperature was uniform and dense with good homogeneity and high crystallinity. The voltage and the anodization time both played significant roles in the formation of TiO2 nanopore-nanotube array film. The optimal voltage was 60 V and the anodization time is less than 30 min by anodizing Ti films in ethylene glycerol containing 0.5% (w) NH4F and 3% (w) H2O. The growth rate of TiO2 nanotube array was as high as 340 nm/min. Moreover, the photocurrent-potential curves, photocurrent response curves and electrochemical impedance spectra results indicated that the TiO2 nanotube array film with the nanoporous structure exhibited a better photo-response ability and photoelectrochemical performance than the ordinary TiO2 nanotube array film. The reason is that the nanoporous structure on the surface of the nanotube array can separate the photo electron-hole pairs more efficiently and completely than the tubular structure.

On Heels and Toes: How Ants Climb with Adhesive Pads and Tarsal Friction Hair Arrays

PubMed Central

Endlein, Thomas; Federle, Walter

2015-01-01

Ants are able to climb effortlessly on vertical and inverted smooth surfaces. When climbing, their feet touch the substrate not only with their pretarsal adhesive pads but also with dense arrays of fine hairs on the ventral side of the 3rd and 4th tarsal segments. To understand what role these different attachment structures play during locomotion, we analysed leg kinematics and recorded single-leg ground reaction forces in Weaver ants (Oecophylla smaragdina) climbing vertically on a smooth glass substrate. We found that the ants engaged different attachment structures depending on whether their feet were above or below their Centre of Mass (CoM). Legs above the CoM pulled and engaged the arolia (‘toes’), whereas legs below the CoM pushed with the 3rd and 4th tarsomeres (‘heels’) in surface contact. Legs above the CoM carried a significantly larger proportion of the body weight than legs below the CoM. Force measurements on individual ant tarsi showed that friction increased with normal load as a result of the bending and increasing side contact of the tarsal hairs. On a rough sandpaper substrate, the tarsal hairs generated higher friction forces in the pushing than in the pulling direction, whereas the reverse effect was found on the smooth substrate. When the tarsal hairs were pushed, buckling was observed for forces exceeding the shear forces found in climbing ants. Adhesion forces were small but not negligible, and higher on the smooth substrate. Our results indicate that the dense tarsal hair arrays produce friction forces when pressed against the substrate, and help the ants to push outwards during horizontal and vertical walking. PMID:26559941

Dense-array concentrator photovoltaic system using non-imaging dish concentrator and crossed compound parabolic concentrator

NASA Astrophysics Data System (ADS)

Chong, Kok-Keong; Yew, Tiong-Keat; Wong, Chee-Woon; Tan, Ming-Hui; Tan, Woei-Chong; Lai, An-Chow; Lim, Boon-Han; Lau, Sing-Liong; Rahman, Faidz Abdul

2015-04-01

Solar concentrating device plays an important role by making use of optical technology in the design, which can be either reflector or lens to deliver high flux of sunlight onto the Concentrator Photovoltaic (CPV) module receiver ranging from hundreds to thousand suns. To be more competitive compared with fossil fuel, the current CPV systems using Fresnel lens and Parabolic dish as solar concentrator that are widely deployed in United States, Australia and Europe are facing great challenge to produce uniformly focused sunlight on the solar cells as to reduce the cost of electrical power generation. The concept of non-imaging optics is not new, but it has not fully explored by the researchers over the world especially in solving the problem of high concentration solar energy, which application is only limited to be a secondary focusing device or low concentration device using Compound Parabolic Concentrator. With the current advancement in the computer processing power, we has successfully invented the non-imaging dish concentrator (NIDC) using numerical simulation method to replace the current parabolic dish as primary focusing device with high solar concentration ratio (more than 400 suns) and large collective area (from 25 to 125 m2). In this paper, we disclose our research and development on dense array CPV system based on non-imaging optics. The geometry of the NIDC is determined using a special computational method. In addition, an array of secondary concentrators, namely crossed compound parabolic concentrators, is also proposed to further focus the concentrated sunlight by the NIDC onto active area of solar cells of the concentrator photovoltaic receiver. The invention maximizes the absorption of concentrated sunlight for the electric power generation system.

On Heels and Toes: How Ants Climb with Adhesive Pads and Tarsal Friction Hair Arrays.

PubMed

Endlein, Thomas; Federle, Walter

2015-01-01

Ants are able to climb effortlessly on vertical and inverted smooth surfaces. When climbing, their feet touch the substrate not only with their pretarsal adhesive pads but also with dense arrays of fine hairs on the ventral side of the 3rd and 4th tarsal segments. To understand what role these different attachment structures play during locomotion, we analysed leg kinematics and recorded single-leg ground reaction forces in Weaver ants (Oecophylla smaragdina) climbing vertically on a smooth glass substrate. We found that the ants engaged different attachment structures depending on whether their feet were above or below their Centre of Mass (CoM). Legs above the CoM pulled and engaged the arolia ('toes'), whereas legs below the CoM pushed with the 3rd and 4th tarsomeres ('heels') in surface contact. Legs above the CoM carried a significantly larger proportion of the body weight than legs below the CoM. Force measurements on individual ant tarsi showed that friction increased with normal load as a result of the bending and increasing side contact of the tarsal hairs. On a rough sandpaper substrate, the tarsal hairs generated higher friction forces in the pushing than in the pulling direction, whereas the reverse effect was found on the smooth substrate. When the tarsal hairs were pushed, buckling was observed for forces exceeding the shear forces found in climbing ants. Adhesion forces were small but not negligible, and higher on the smooth substrate. Our results indicate that the dense tarsal hair arrays produce friction forces when pressed against the substrate, and help the ants to push outwards during horizontal and vertical walking.

Package architecture and component design for an implanted neural stimulator with closed loop control.

PubMed

Bjune, Caroline K; Marinis, Thomas F; Brady, Jeanne M; Moran, James; Wheeler, Jesse; Sriram, Tirunelveli S; Parks, Philip D; Widge, Alik S; Dougherty, Darin D; Eskandar, Emad N

2015-08-01

An implanted neural stimulator with closed loop control requires electrodes for stimulation pulses and recording neuron activity. Our system features arrays of 64 electrodes. Each electrode can be addressed through a cross bar switch, to enable it to be used for stimulation or recording. This electrode switch, a bank of low noise amplifiers with an integrated analog to digital converter, power conditioning electronics, and a communications and control gate array are co-located with the electrode array in a 14 millimeter diameter satellite package that is designed to be flush mounted in a skull burr hole. Our system features five satellite packages connected to a central hub processor-controller via ten conductor cables that terminate in a custom designed, miniaturized connector. The connector incorporates features of high reliability, military grade devices and utilizes three distinct seals to isolate the contacts from fluid permeation. The hub system is comprised of a connector header, hermetic electronics package, and rechargeable battery pack, which are mounted on and electrically interconnected by a flexible circuit board. The assembly is over molded with a compliant silicone rubber. The electronics package contains two antennas, a large coil, used for recharging the battery and a high bandwidth antenna that is used to download data and update software. The package is assembled from two machined alumina pieces, a flat base with brazed in, electrical feed through pins and a rectangular cover with rounded corners. Titanium seal rings are brazed onto these two pieces so that they can be sealed by laser welding. A third system antenna is incorporated in the flexible circuit board. It is used to communicate with an externally worn control package, which monitors the health of the system and allows both the user and clinician to control or modify various system function parameters.

Deeper Cochlear Implant Electrode Insertion Angle Improves Detection of Musical Sound Quality Deterioration Related to Bass Frequency Removal.

PubMed

Roy, Alexis T; Penninger, Richard T; Pearl, Monica S; Wuerfel, Waldemar; Jiradejvong, Patpong; Carver, Courtney; Buechner, Andreas; Limb, Charles J

2016-02-01

Cochlear implant (CI) electrode arrays typically do not reach the most apical regions of the cochlea that intrinsically encode low frequencies. This may contribute to diminished implant-mediated musical sound quality perception. The objective of this study was to assess the effect of varying degrees of apical cochlear stimulation (measured by angular insertion depth) on musical sound quality discrimination. Increased apical cochlear stimulation will improve low-frequency perception and musical sound quality discrimination. Standard (31.5 mm, n = 17) and medium (24 mm, n = 8) array Med-EL CI users, and normal hearing (NH) listeners (n = 16) participated. Imaging confirmed angular insertion depth. Participants completed a musical discrimination task in which they listened to a real-world musical stimulus (labeled reference) and provided sound quality ratings to versions of the reference, which included a hidden reference and test stimuli with increasing amounts of low-frequency removal. Scores for each CI users were calculated on the basis of how much their ratings differed from NH listeners for each stimulus version. Medium array and standard users had significantly different insertion depths (389.4 ± 64.5 and 583.9 ± 78.5 degrees, respectively; p <  .001). A significant Pearson's correlation was observed between angular insertion depth and the hidden reference scores (p < 0.05). CI users with greater apical stimulation made sound quality discriminations that more closely resembled those of NH controls for stimuli that contained low frequencies (< 200 Hz of information). These findings suggest that increased apical cochlear stimulation improves musical low-frequency perception, which may provide a more satisfactory music listening experience for CI users.

Signal processing methods for reducing artifacts in microelectrode brain recordings caused by functional electrical stimulation

NASA Astrophysics Data System (ADS)

Young, D.; Willett, F.; Memberg, W. D.; Murphy, B.; Walter, B.; Sweet, J.; Miller, J.; Hochberg, L. R.; Kirsch, R. F.; Ajiboye, A. B.

2018-04-01

Objective. Functional electrical stimulation (FES) is a promising technology for restoring movement to paralyzed limbs. Intracortical brain-computer interfaces (iBCIs) have enabled intuitive control over virtual and robotic movements, and more recently over upper extremity FES neuroprostheses. However, electrical stimulation of muscles creates artifacts in intracortical microelectrode recordings that could degrade iBCI performance. Here, we investigate methods for reducing the cortically recorded artifacts that result from peripheral electrical stimulation. Approach. One participant in the BrainGate2 pilot clinical trial had two intracortical microelectrode arrays placed in the motor cortex, and thirty-six stimulating intramuscular electrodes placed in the muscles of the contralateral limb. We characterized intracortically recorded electrical artifacts during both intramuscular and surface stimulation. We compared the performance of three artifact reduction methods: blanking, common average reference (CAR) and linear regression reference (LRR), which creates channel-specific reference signals, composed of weighted sums of other channels. Main results. Electrical artifacts resulting from surface stimulation were 175  ×  larger than baseline neural recordings (which were 110 µV peak-to-peak), while intramuscular stimulation artifacts were only 4  ×  larger. The artifact waveforms were highly consistent across electrodes within each array. Application of LRR reduced artifact magnitudes to less than 10 µV and largely preserved the original neural feature values used for decoding. Unmitigated stimulation artifacts decreased iBCI decoding performance, but performance was almost completely recovered using LRR, which outperformed CAR and blanking and extracted useful neural information during stimulation artifact periods. Significance. The LRR method was effective at reducing electrical artifacts resulting from both intramuscular and surface FES, and almost completely restored iBCI decoding performance (>90% recovery for surface stimulation and full recovery for intramuscular stimulation). The results demonstrate that FES-induced artifacts can be easily mitigated in FES  +  iBCI systems by using LRR for artifact reduction, and suggest that the LRR method may also be useful in other noise reduction applications.

Thermoelctric Properties of Bi and Bismuth Telluride Composites

NASA Astrophysics Data System (ADS)

Huber, Tito E.; Calcao, Ricky

1998-03-01

It has been suggested that microengineering traditional thermoelectric materials into composites may leadto asignificant improvement in their thermoelectric performance. One approach for the fabrication of nanostructured materials is the utilization of nanochannel insulators as a matrix for the synthesis of dense composites using high pressure injection of the melt. We will discuss the synthesis and structural properties of oriented Bi and Bismuth Telluride wire arrays prepared with this technique. Funded by the Army Research Office.

Discrete microfluidics: Reorganizing droplet arrays at a bend

NASA Astrophysics Data System (ADS)

Surenjav, Enkhtuul; Herminghaus, Stephan; Priest, Craig; Seemann, Ralf

2009-10-01

Microfluidic manipulation of densely packed droplet arrangements (i.e., gel emulsions) using sharp microchannel bends was studied as a function of bend angle, droplet volume fraction, droplet size, and flow velocity. Emulsion reorganization was found to be specifically dependent on the pathlength that the droplets are forced to travel as they navigate the bend under spatial confinement. We describe how bend-induced droplet displacements might be exploited in complex, droplet-based microfluidics.

Textured carbon surfaces on copper by sputtering

NASA Technical Reports Server (NTRS)

Curren, A. N. (Inventor); Jensen, K. A. (Inventor); Roman, R. F. (Inventor)

1986-01-01

A very thin layer of highly textured carbon is applied to a copper surface by a triode sputtering process. A carbon target and a copper substrate are simultaneously exposed to an argon plasma in a vacuum chamber. The resulting carbon surface is characterized by a dense, random array of needle like spires or peaks which extend perpendicularly from the copper surface. The coated copper is especially useful for electrode plates in multistage depressed collectors.

The CAFE Experiment: A Joint Seismic and MT Investigation of the Cascadia Subduction System

DTIC Science & Technology

2013-02-01

In this thesis we present results from inversion of data using dense arrays of collocated seismic and magnetotelluric stations located in the Cascadia...implicit in the standard MT inversion provides tools that enable us to generate a more accurate MT model. This final MT model clearly demonstrates...references within, Hacker, 2008) have given us the tools to better interpret geophysical evidence. Improvements in the thermal modeling of subduction zones

Electric crosstalk impairs spatial resolution of multi-electrode arrays in retinal implants

NASA Astrophysics Data System (ADS)

Wilke, R. G. H.; Khalili Moghadam, G.; Lovell, N. H.; Suaning, G. J.; Dokos, S.

2011-08-01

Active multi-electrode arrays are used in vision prostheses, including optic nerve cuffs and cortical and retinal implants for stimulation of neural tissue. For retinal implants, arrays with up to 1500 electrodes are used in clinical trials. The ability to convey information with high spatial resolution is critical for these applications. To assess the extent to which spatial resolution is impaired by electric crosstalk, finite-element simulation of electric field distribution in a simplified passive tissue model of the retina is performed. The effects of electrode size, electrode spacing, distance to target cells, and electrode return configuration (monopolar, tripolar, hexagonal) on spatial resolution is investigated in the form of a mathematical model of electric field distribution. Results show that spatial resolution is impaired with increased distance from the electrode array to the target cells. This effect can be partly compensated by non-monopolar electrode configurations and larger electrode diameters, albeit at the expense of lower pixel densities due to larger covering areas by each stimulation electrode. In applications where multi-electrode arrays can be brought into close proximity to target cells, as presumably with epiretinal implants, smaller electrodes in monopolar configuration can provide the highest spatial resolution. However, if the implantation site is further from the target cells, as is the case in suprachoroidal approaches, hexagonally guarded electrode return configurations can convey higher spatial resolution. This paper was originally submitted for the special issue containing contributions from the Sixth Biennial Research Congress of The Eye and the Chip.

Time-resolved large-scale volumetric pressure fields of an impinging jet from dense Lagrangian particle tracking

NASA Astrophysics Data System (ADS)

Huhn, F.; Schanz, D.; Manovski, P.; Gesemann, S.; Schröder, A.

2018-05-01

Time-resolved volumetric pressure fields are reconstructed from Lagrangian particle tracking with high seeding concentration using the Shake-The-Box algorithm in a perpendicular impinging jet flow with exit velocity U=4 m/s (Re˜ 36,000) and nozzle-plate spacing H/D=5. Helium-filled soap bubbles are used as tracer particles which are illuminated with pulsed LED arrays. A large measurement volume has been covered (cloud of tracked particles in a volume of 54 L, ˜ 180,000 particles). The reconstructed pressure field has been validated against microphone recordings at the wall with high correlation coefficients up to 0.88. In a reduced measurement volume (13 L), dense Lagrangian particle tracking is shown to be feasable up to the maximal possible jet velocity of U=16 m/s.

Densely packed aluminum-silver nanohelices as an ultra-thin perfect light absorber

PubMed Central

Jen, Yi-Jun; Huang, Yu-Jie; Liu, Wei-Chih; Lin, Yueh Weng

2017-01-01

Metals have been formed into nanostructures to absorb light with high efficiency through surface plasmon resonances. An ultra-thin plasmonic structure that exhibits strong absorption over wide ranges of wavelengths and angles of incidence is sought. In this work, a nearly perfect plasmonic nanostructure is fabricated using glancing angle deposition. The difference between the morphologies of obliquely deposited aluminum and silver nanohelices is exploited to form a novel three-dimensional structure, which is an aluminum-silver nanohelix array on a pattern-free substrate. With a thickness of only 470 nm, densely distributed nanohelices support rod-to-rod localized surface plasmons for broadband and polarization-independent light extinction. The extinctance remains high over wavelengths from 400 nm to 2000 nm and angles of incidence from 0° to 70°. PMID:28045135

Fabrication of cell container arrays with overlaid surface topographies.

PubMed

Truckenmüller, Roman; Giselbrecht, Stefan; Escalante-Marun, Maryana; Groenendijk, Max; Papenburg, Bernke; Rivron, Nicolas; Unadkat, Hemant; Saile, Volker; Subramaniam, Vinod; van den Berg, Albert; van Blitterswijk, Clemens; Wessling, Matthias; de Boer, Jan; Stamatialis, Dimitrios

2012-02-01

This paper presents cell culture substrates in the form of microcontainer arrays with overlaid surface topographies, and a technology for their fabrication. The new fabrication technology is based on microscale thermoforming of thin polymer films whose surfaces are topographically prepatterned on a micro- or nanoscale. For microthermoforming, we apply a new process on the basis of temporary back moulding of polymer films and use the novel concept of a perforated-sheet-like mould. Thermal micro- or nanoimprinting is applied for prepatterning. The novel cell container arrays are fabricated from polylactic acid (PLA) films. The thin-walled microcontainer structures have the shape of a spherical calotte merging into a hexagonal shape at their upper circumferential edges. In the arrays, the cell containers are arranged densely packed in honeycomb fashion. The inner surfaces of the highly curved container walls are provided with various topographical micro- and nanopatterns. For a first validation of the microcontainer arrays as in vitro cell culture substrates, C2C12 mouse premyoblasts are cultured in containers with microgrooved surfaces and shown to align along the grooves in the three-dimensional film substrates. In future stem-cell-biological and tissue engineering applications, microcontainers fabricated using the proposed technology may act as geometrically defined artificial microenvironments or niches.

Detection of Old Mine Tunnels in Mexico City Highlands by Electric Resistivity Image Methods

NASA Astrophysics Data System (ADS)

Chavez, R. E.; Tejero, A.; Cifuentes-Nava, G.; HernaNdez-Quintero, J.

2013-12-01

Electrical Resistivity Tomography (ERT) methods have been applied to study cavities or subsurface subsidence threatening urbanized areas. Unfortunately, ERT-3D techniques carried out on heavily urbanized areas become a difficult task, since parallel ERT arrays cannot be deployed. Then, a conventional regular grid cannot be carried out. We present a subsidence problem located in a densely populated portion of Mexico City highlands. Since the damaged houses are in the middle of a highly populated low-class neighborhood, an unconventional ERT array had to be applied. At first, a ';T'-array formed by two perpendicular transects was applied, deployed within a small alley, that stretched from the house entrance. This study determined a tubular structure beneath the houses following an irregular path at depth. Finally, houses were demolished due to the extensive damaged in their foundations. This made possible to carry out a second ERT-3D study, which included a dipolar array called ';L' and ';Corner' arrays. Such a new work defined a similar tubular structure. The cavity entrance was discovered, when excavations were made, although its precise shape could not be defined. The ERT-3D interpretation contributed to locate and accurately determine the geometrical characteristics of the geological feature that caused the collapse of dwellings.

Status of air-shower measurements with sparse radio arrays

NASA Astrophysics Data System (ADS)

Schröder, Frank G.

2017-03-01

This proceeding gives a summary of the current status and open questions of the radio technique for cosmic-ray air showers, assuming that the reader is already familiar with the principles. It includes recent results of selected experiments not present at this conference, e.g., LOPES and TREND. Current radio arrays like AERA or Tunka-Rex have demonstrated that areas of several km2 can be instrumented for reasonable costs with antenna spacings of the order of 200m. For the energy of the primary particle such sparse antenna arrays can already compete in absolute accuracy with other precise techniques, like the detection of air-fluorescence or air-Cherenkov light. With further improvements in the antenna calibration, the radio detection might become even more accurate. For the atmospheric depth of the shower maximum, Xmax, currently only the dense array LOFAR features a precision similar to the fluorescence technique, but analysis methods for the radio measurement of Xmax are still under development. Moreover, the combination of radio and muon measurements is expected to increase the accuracy of the mass composition, and this around-the-clock recording is not limited to clear nights as are the light-detection methods. Consequently, radio antennas will be a valuable add-on for any air shower array targeting the energy range above 100 PeV.

Interfacial self-assembled functional nanoparticle array: a facile surface-enhanced Raman scattering sensor for specific detection of trace analytes.

PubMed

Zhang, Kun; Ji, Ji; Li, Yixin; Liu, Baohong

2014-07-01

Surface-enhanced Raman scattering (SERS) has proven to be promising for the detection of trace analytes; however, the precise nanofabrication of a specific and sensitive plasmonic SERS-active substrate is still a major challenge that limits the scope of its applications. In this work, gold nanoparticles are self-assembled into densely packed two-dimensional arrays at a liquid/liquid interface between dimethyl carbonate and water in the absence of template controller molecules. Both the simulation and experiment results show that the particles within these film-like arrays exhibit strong electromagnetic coupling and enable large amplification of Raman signals. In order to realize the level of sensing specificity, the surface chemistry of gold nanoparticles (Au NPs) is rationally tailored by incorporating an appropriate chemical moiety that specifically captures molecules of interest. The ease of fabrication and good uniformity make this platform ideal for in situ SERS sensing of trace targets in complex samples.

Effect of low-level laser stimulation on EEG.

PubMed

Wu, Jih-Huah; Chang, Wen-Dien; Hsieh, Chang-Wei; Jiang, Joe-Air; Fang, Wei; Shan, Yi-Chia; Chang, Yang-Chyuan

2012-01-01

Conventional laser stimulation at the acupoint can induce significant brain activation, and the activation is theoretically conveyed by the sensory afferents. Whether the insensible low-level Laser stimulation outside the acupoint could also evoke electroencephalographic (EEG) changes is not known. We designed a low-level laser array stimulator (6 pcs laser diode, wavelength 830 nm, output power 7 mW, and operation frequency 10 Hz) to deliver insensible laser stimulations to the palm. EEG activities before, during, and after the laser stimulation were collected. The amplitude powers of each EEG frequency band were analyzed. We found that the low-level laser stimulation was able to increase the power of alpha rhythms and theta waves, mainly in the posterior head regions. These effects lasted at least 15 minutes after cessation of the laser stimulation. The amplitude power of beta activities in the anterior head regions decreased after laser stimulation. We thought these EEG changes comparable to those in meditation.

Mechanical Designs and Developement of Advanced ACT: A Transfomative Upgrade to the ACTPol Receiver on the Atacama Cosmology Telescope.

NASA Astrophysics Data System (ADS)

Ward, Jonathan; Advanced ACT Collaboration, NASA Space Technology Research Fellowship

2017-06-01

The Atacama Cosmology Telescope is a six-meter diameter telescope located at 17,000 feet (5,200 meters) on Cerro Toco in the Andes Mountains of northern Chile. The next generation Advanced ACT (AdvACT) experiment is currently underway and will consist of three multichroic TES bolometer arrays operating together, totaling 5800 detectors on the sky. Each array will be sensitive to two frequency bands: a high frequency (HF) array at 150 and 230 GHz, two middle frequency (MF) arrays at 90 and 150 GHz, and a low frequency (LF) array at 28 and 41 GHz. The AdACT detector arrays will feature a revamped design when compared to ACTPol, including a transition to 150mm wafers equipped with multichroic pixels, allowing for a more densely packed focal plane. Each set of detectors consists of a feedhorn array of stacked silicon wafers which form a corrugated profile leading to each pixel. This is then followed by a four-piece detector stack assembly of silicon wafers which includes a waveguide interface plate, detector wafer, backshort cavity plate, and backshort cap. Each array is housed in a custom designed structure manufactured out of gold-plated, high purity copper. In addition to the detector array assembly, the array package also encloses the majority of our readout electronics. We present the full mechanical design of the AdvACT HF and MF detector array packages along with a detailed look at the detector array assemblies. We also highlight the use of continuously rotating warm half-wave plates (HWPs) at the front of the AdvACT receiver. We review the design of the rotation system and also early pipeline data analysis results. This experiment will also make use of extensive hardware and software previously developed for ACT, which will be modified to incorporate the new AdvACT instruments. Therefore, we discuss the integration of all AdvACT instruments with pre-existing ACTPol infrastructure.

Implications from Meteoric and Volcanic Infrasound Measured in the Netherlands

NASA Astrophysics Data System (ADS)

Evers, L.

2003-12-01

Infrasound observations started in the Netherlands in 1986. Since then, several array configurations and instruments have been developed, tested and made operational. Currently, three infrasound arrays are continuously measuring infrasound with in-house developed microbarometers. The array apertures vary from 30 to 1500 meters and the number of instruments from 6 to 16 microbarometers. The inter-array distance ranges from 50 up to 150 km. This dense network of infrasound arrays is used to distinguish between earthquakes and sources in the atmosphere. Sonic booms, for example, can be experienced in the same manner as small (gas induced) earthquakes. Furthermore, Comprehensive Nuclear-Test-Ban Treaty (CTBT) related research is done. Meteors are one of the few natural impulsive sources generating energy in kT TNT equivalent range. Therefore, the study of meteors is essential to the CTBT where infrasound is applied as monitoring technique. Studies of meteors in the Netherlands have shown the capability of infrasound to trace a meteor through the stratosphere. The propagation of infrasound is in first order dependent on the wind and temperature structure of the atmosphere. The meteor's path could be reconstructed by using ECMWF atmospheric models for wind and temperature. The results were compared to visual observations, confirming the location, direction and reported origin time. The accuracy of the localization mainly depends on the applied atmospheric model and array resolution. Successfully applying infrasound depends on the array configuration that should be based on the -frequency depend- spatial coherence of the signals of interest. The array aperture and inter-element distance will play a decisive role in detecting low signal-to-noise ratios. This is shown by results from studies on volcanic infrasound from Mt. Etna (Italy) detected in the Netherlands. Sub-array processing on the 16 element array revealed an increased detectability of infrasound for small aperture, 800 m, arrays, compared to large aperture, 1500 m, arrays.

Fabrication and Characterization of Vertically Aligned ZnO Nanorod Arrays via Inverted Monolayer Colloidal Crystals Mask

NASA Astrophysics Data System (ADS)

Chen, Cheng; Ding, Taotao; Qi, Zhiqiang; Zhang, Wei; Zhang, Jun; Xu, Juan; Chen, Jingwen; Dai, Jiangnan; Chen, Changqing

2018-04-01

The periodically ordered ZnO nanorod (NR) arrays have been successfully synthesized via a hydrothermal approach on the silicon substrates by templating of the TiO2 ring deriving from the polystyrene (PS) nanosphere monolayer colloidal crystals (MCC). With the inverted MCC mask, sol-gel-derived ZnO seeds could serve as the periodic nucleation positions for the site-specific growth of ZnO NRs. The large-scale patterned arrays of single ZnO NR with good side-orientation can be readily produced. According to the experimental results, the as-integrated ZnO NR arrays showed an excellent crystal quality and optical property, very suitable for optoelectronic applications such as stimulated emitters and ZnO photonic crystal devices.

Selective recurrent laryngeal nerve stimulation using a penetrating electrode array in the feline model.

PubMed

Haidar, Yarah M; Sahyouni, Ronald; Moshtaghi, Omid; Wang, Beverly Y; Djalilian, Hamid R; Middlebrooks, John C; Verma, Sunil P; Lin, Harrison W

2017-10-31

Laryngeal muscles (LMs) are controlled by the recurrent laryngeal nerve (RLN), injury of which can result in vocal fold (VF) paralysis (VFP). We aimed to introduce a bioelectric approach to selective stimulation of LMs and graded muscle contraction responses. Acute experiments in cats. The study included six anesthetized cats. In four cats, a multichannel penetrating microelectrode array (MEA) was placed into an uninjured RLN. For RLN injury experiments, one cat received a standardized hemostat-crush injury, and one cat received a transection-reapproximation injury 4 months prior to testing. In each experiment, three LMs (thyroarytenoid, posterior cricoarytenoid, and cricothyroid muscles) were monitored with an electromyographic (EMG) nerve integrity monitoring system. Electrical current pulses were delivered to each stimulating channel individually. Elicited EMG voltage outputs were recorded for each muscle. Direct videolaryngoscopy was performed for visualization of VF movement. Stimulation through individual channels led to selective activation of restricted nerve populations, resulting in selective contraction of individual LMs. Increasing current levels resulted in rising EMG voltage responses. Typically, activation of individual muscles was successfully achieved via single placement of the MEA by selection of appropriate stimulation channels. VF abduction was predominantly observed on videolaryngoscopy. Nerve histology confirmed injury in cases of RLN crush and transection experiments. We demonstrated the ability of a penetrating MEA to selectively stimulate restricted fiber populations within the feline RLN and selectively elicit contractions of discrete LMs in both acute and injury-model experiments, suggesting a potential role for intraneural MEA implantation in VFP management. NA Laryngoscope, 2017. © 2017 The American Laryngological, Rhinological and Otological Society, Inc.

An analysis of current source density profiles activated by local stimulation in the mouse auditory cortex in vitro.

PubMed

Yamamura, Daiki; Sano, Ayaka; Tateno, Takashi

2017-03-15

To examine local network properties of the mouse auditory cortex in vitro, we recorded extracellular spatiotemporal laminar profiles driven by short electric local stimulation on a planar multielectrode array substrate. The recorded local field potentials were subsequently evaluated using current source density (CSD) analysis to identify sources and sinks. Current sinks are thought to be an indicator of net synaptic current in the small volume of cortex surrounding the recording site. Thus, CSD analysis combined with multielectrode arrays enabled us to compare mean synaptic activity in response to small current stimuli on a layer-by-layer basis. We also used senescence-accelerated mice (SAM), some strains of which show earlier onset of age-related hearing loss, to examine the characteristic spatiotemporal CSD profiles stimulated by electrodes in specific cortical layers. Thus, the CSD patterns were classified into several clusters based on stimulation sites in the cortical layers. We also found some differences in CSD patterns between the two SAM strains in terms of aging according to principle component analysis with dimension reduction. For simultaneous two-site stimulation, we modeled the obtained CSD profiles as a linear superposition of the CSD profiles to individual single-site stimulation. The model analysis indicated the nonlinearity of spatiotemporal integration over stimulus-driven activity in a layer-specific manner. Finally, on the basis of these results, we discuss the auditory cortex local network properties and the effects of aging on these mouse strains. Copyright © 2017 Elsevier B.V. All rights reserved.

Development of very large electrode arrays for epiretinal stimulation (VLARS)

PubMed Central

2014-01-01

Background Retinal implants have been developed to treat blindness causing retinal degenerations such as Retinitis pigmentosa (RP). The retinal stimulators are covering only a small portion of the retina usually in its center. To restore not only central vision but also a useful visual field retinal stimulators need to cover a larger area of the retina. However, large area retinal stimulators are much more difficult to implant into an eye. Some basic questions concerning this challenge should be answered in a series of experiments. Methods Large area retinal stimulators were fabricated as flexible multielectrode arrays (MEAs) using silicon technology with polyimide as the basic material for the substrate. Electrodes were made of gold covered with reactively sputtered iridium oxide. Several prototype designs were considered and implanted into enucleated porcine eyes. The prototype MEAs were also used as recording devices. Results Large area retinal stimulator MEAs were fabricated with a diameter of 12 mm covering a visual angle of 37.6° in a normal sighted human eye. The structures were flexible enough to be implanted in a folded state through an insertion nozzle. The implants could be positioned onto the retinal surface and fixated here using a retinal tack. Recording of spontaneous activity of retinal neurons was possible in vitro using these devices. Conclusions Large flexible MEAs covering a wider area of the retina as current devices could be fabricated using silicon technology with polyimide as a base material. Principal surgical techniques were established to insert such large devices into an eye and the devices could also be used for recording of retinal neural activity. PMID:24502253

Metallic taste from electrical and chemical stimulation.

PubMed

Lawless, Harry T; Stevens, David A; Chapman, Kathryn W; Kurtz, Anne

2005-03-01

A series of three experiments investigated the nature of metallic taste reports after stimulation with solutions of metal salts and after stimulation with metals and electric currents. To stimulate with electricity, a device was fabricated consisting of a small battery affixed to a plastic handle with the anode side exposed for placement on the tongue or oral tissues. Intensity of taste from metals and batteries was dependent upon the voltage and was more robust in areas dense in fungiform papillae. Metallic taste was reported from stimulation with ferrous sulfate solutions, from metals and from electric stimuli. However, reports of metallic taste were more frequent when the word 'metallic' was presented embedded in a list of choices, as opposed to simple free-choice labeling. Intensity decreased for ferrous sulfate when the nose was occluded, consistent with a decrease in retronasal smell, as previously reported. Intensity of taste evoked by copper metal, bimetallic stimuli (zinc/copper) or small batteries (1.5-3 V) was not affected by nasal occlusion. This difference suggests two distinct mechanisms for evocation of metallic taste reports, one dependent upon retronasal smell and a second mediated by oral chemoreceptors.

Isolation and characterization of nonhistone chromosomal protein C-14 which stimulates RNA synthesis.

PubMed

James, G T; Yeoman, L C; Matsui, S i; Goldberg, A H; Busch, H

1977-05-31

The nonhistone chromatin protein, C-14, was extracted from chromatin of Novikoff hepatoma ascites cells and isolated in high purity as shown by its migration as a single dense spot on two-dimensional polyacrylamide gels. Its mobility on sodium dodecyl sulfate gels is consistent with a molecular weight of approximately 70 000. The amino acid composition shows that protein C-14 has an acidic:basic amino acid ratio of 1.8. Its amino terminal amino acid is lysine. Protein C-14 stimulated the incorporation of [3H]UMP into RNA by approximately 30% when added to naked DNA and homologous RNA polymerase I. A 30% stimulation of [3H]UMP incorporation into RNA was also found when protein C-14 was added to an E. coli RNA polymerase system containing either E. coli or Novikoff hepatoma DNA.

Optogenetic stimulation of multiwell MEA plates for neural and cardiac applications

NASA Astrophysics Data System (ADS)

Clements, Isaac P.; Millard, Daniel C.; Nicolini, Anthony M.; Preyer, Amanda J.; Grier, Robert; Heckerling, Andrew; Blum, Richard A.; Tyler, Phillip; McSweeney, K. M.; Lu, Yi-Fan; Hall, Diana; Ross, James D.

2016-03-01

Microelectrode array (MEA) technology enables advanced drug screening and "disease-in-a-dish" modeling by measuring the electrical activity of cultured networks of neural or cardiac cells. Recent developments in human stem cell technologies, advancements in genetic models, and regulatory initiatives for drug screening have increased the demand for MEA-based assays. In response, Axion Biosystems previously developed a multiwell MEA platform, providing up to 96 MEA culture wells arrayed into a standard microplate format. Multiwell MEA-based assays would be further enhanced by optogenetic stimulation, which enables selective excitation and inhibition of targeted cell types. This capability for selective control over cell culture states would allow finer pacing and probing of cell networks for more reliable and complete characterization of complex network dynamics. Here we describe a system for independent optogenetic stimulation of each well of a 48-well MEA plate. The system enables finely graded control of light delivery during simultaneous recording of network activity in each well. Using human induced pluripotent stem cell (hiPSC) derived cardiomyocytes and rodent primary neuronal cultures, we demonstrate high channel-count light-based excitation and suppression in several proof-of-concept experimental models. Our findings demonstrate advantages of combining multiwell optical stimulation and MEA recording for applications including cardiac safety screening, neural toxicity assessment, and advanced characterization of complex neuronal diseases.

Towards multifocal ultrasonic neural stimulation: pattern generation algorithms

NASA Astrophysics Data System (ADS)

Hertzberg, Yoni; Naor, Omer; Volovick, Alexander; Shoham, Shy

2010-10-01

Focused ultrasound (FUS) waves directed onto neural structures have been shown to dynamically modulate neural activity and excitability, opening up a range of possible systems and applications where the non-invasiveness, safety, mm-range resolution and other characteristics of FUS are advantageous. As in other neuro-stimulation and modulation modalities, the highly distributed and parallel nature of neural systems and neural information processing call for the development of appropriately patterned stimulation strategies which could simultaneously address multiple sites in flexible patterns. Here, we study the generation of sparse multi-focal ultrasonic distributions using phase-only modulation in ultrasonic phased arrays. We analyse the relative performance of an existing algorithm for generating multifocal ultrasonic distributions and new algorithms that we adapt from the field of optical digital holography, and find that generally the weighted Gerchberg-Saxton algorithm leads to overall superior efficiency and uniformity in the focal spots, without significantly increasing the computational burden. By combining phased-array FUS and magnetic-resonance thermometry we experimentally demonstrate the simultaneous generation of tightly focused multifocal distributions in a tissue phantom, a first step towards patterned FUS neuro-modulation systems and devices.

Discovery of non-volcanic tremor and contribution to earth science by NIED Hi-net

NASA Astrophysics Data System (ADS)

Obara, K.

2015-12-01

Progress of seismic observation network brings breakthroughs in the earth science at each era. High sensitivity seismograph network (Hi-net) was constructed by National Research Institute for Earth Science and Disaster Prevention (NIED) as a national project in order to improve the detection capability of microearthquake after disastrous 1995 Kobe earthquake. Hi-net has been contributing to not only monitoring of seismicity but also producing many research results like as discoveries of non-volcanic tremor and other slow earthquakes. More important thing is that we have continued to make efforts to monitor all of data visually and effectively. The discovery of tremor in southwest Japan stimulated PGC researchers to search similar seismic signature in Cascadia because of a couple of common features in the tremor in Japan and slow slip event (SSE) they already discovered in Cascadia. At last, episodic tremor and slip (ETS) was discovered, then the SSE associated with tremor was also detected in Japan by using the tilting data measured by high-sensitivity accelerometer attached with the Hi-net. This coupling phenomena strengthened the connection between seismology and geodesy. Widely separated spectrum of tremor and SSE motivated us to search intervened phenomena, then we found very low frequency earthquake during ETS episode. These slow earthquakes obey a scaling law different from ordinary earthquake. This difference is very important to resolve the earthquake physics. Hi-net is quite useful for not only three-dimensional imaging of underground structure beneath the Japan Islands, but also resolving deep Earth interior by using teleseismic events or ambient noises and source rupture process of large earthquakes by using back-projection analysis as a remote array. Hi-net will continue to supply unexpected new discoveries. I expect that multiple installation of similar dense seismic array in the world will give us great opportunity to discover more important and explore a new regime in the earth science.

Tracking fin whale calls offshore the Galicia Margin, North East Atlantic Ocean.

PubMed

Gaspà Rebull, Oriol; Díaz Cusí, Jordi; Ruiz Fernández, Mario; Gallart Muset, Josep

2006-10-01

Data recorded during a temporary deployment of ocean bottom seismometers (OBSs) are used in this study to monitor the presence of fin whales around the array. In the summer of 2003, ten OBSs were placed 250 km from the NW coast of Iberia in the Galicia Margin, NE Atlantic Ocean for a period of one month. The recorded data set provided a large variety of signals, including fin whale vocalizations identified by their specific acoustic signature. The use of a dense array of seafloor receivers allowed investigation into the locations and tracks of the signal-generating whales using a seismological hypocentral location code. Individual pulses of different sequences have been chosen to study such tracks. Problems related to the correct identification of pulses, discrimination between direct and multiple arrivals, and the presence of more than one individual have been considered prior to location. Fin calls were concentrated in the last two weeks of the deployment and the locations were spread around the area covered by the array. These results illustrate that, besides its classical seismological aim, deployment of semipermanent seafloor seismic arrays can also provide valuable data for marine mammal behavior studies.

Plasmonic Biosensor Based on Vertical Arrays of Gold Nanoantennas.

PubMed

Klinghammer, Stephanie; Uhlig, Tino; Patrovsky, Fabian; Böhm, Matthias; Schütt, Julian; Pütz, Nils; Baraban, Larysa; Eng, Lukas M; Cuniberti, Gianaurelio

2018-06-25

Implementing large arrays of gold nanowires as functional elements of a plasmonic biosensor is an important task for future medical diagnostic applications. Here we present a microfluidic-channel-integrated sensor for the label-free detection of biomolecules, relying on localized surface plasmon resonances. Large arrays (∼1 cm 2 ) of vertically aligned and densely packed gold nanorods to receive, locally confine, and amplify the external optical signal are used to allow for reliable biosensing. We accomplish this by monitoring the change of the optical nanostructure resonance in the presence of biomolecules within the tight focus area above the nanoantennas, combined with a surface treatment of the nanowires for a specific binding of the target molecules. As a first application, we detect the binding kinetics of two distinct DNA strands as well as the following hybridization of two complementary strands (cDNA) with different lengths (25 and 100 bp). Upon immobilization, a redshift of 1 nm was detected; further backfilling and hybridization led to a peak shift of additional 2 and 5 nm for 25 and 100 bp, respectively. We believe that this work gives deeper insight into the functional understanding and technical implementation of a large array of gold nanowires for future medical applications.

ALMA observations towards G023.01-00.41 .

NASA Astrophysics Data System (ADS)

Sanna, A.; Moscadelli, L.; Cesaroni, R.; Caratti o Garatti, A.; Menten, K. M.; Kölligan, A.; Kuiper, R.

We want to understand whether or not young stars, with masses of tens of Solar masses, can form in the disk accretion scenario. This challenge requires to resolve the spatial morphology of gas and dust within a few 1000 au of a massive young stellar object, and to measure the gas kinematics with respect to the star. Also, because the gas kinematics near the young star can be a mixture of rotating, expanding, and infalling motions all together, to separate each velocity component it is necessary to map the emission of various gas tracers, as well as to image the circumstellar gas at different distances from the star. With this in mind, we made use of the Atacama Large Millimeter Array (ALMA) at wavelengths near 1 mm, with the aim to image the dense molecular gas in the vicinity of a well-known O-type young star. We previously observed this source with the Submillimeter Array (SMA), at scales of about 0.1 pc, with the Karl G. Jansky Very Large Array (VLA), at scales of the order of 1000 au, and with the Very Long Baseline Array (VLBA) and European VLBI Network (EVN), at scales of a few au.

Focusing of relativistic electrons in dense plasma using a resistivity-gradient-generated magnetic switchyard.

PubMed

Robinson, A P L; Key, M H; Tabak, M

2012-03-23

A method for producing a self-generated magnetic focussing structure for a beam of laser-generated relativistic electrons using a complex array of resistivity gradients is proposed and demonstrated using numerical simulations. The array of resistivity gradients is created by using a target consisting of alternating layers of different Z material. This new scheme is capable of effectively focussing the fast electrons even when the source is highly divergent. The application of this technique to cone-guided fast ignition inertial confinement fusion is considered, and it is shown that it may be possible to deposit over 25% of the fast electron energy into a hot spot even when the fast electron divergence angle is very large (e.g., 70° half-angle).

Efficient energy absorption of intense ps-laser pulse into nanowire target

NASA Astrophysics Data System (ADS)

Habara, H.; Honda, S.; Katayama, M.; Sakagami, H.; Nagai, K.; Tanaka, K. A.

2016-06-01

The interaction between ultra-intense laser light and vertically aligned carbon nanotubes is investigated to demonstrate efficient laser-energy absorption in the ps laser-pulse regime. Results indicate a clear enhancement of the energy conversion from laser to energetic electrons and a simultaneously small plasma expansion on the surface of the target. A two-dimensional plasma particle calculation exhibits a high absorption through laser propagation deep into the nanotube array, even for a dense array whose structure is much smaller than the laser wavelength. The propagation leads to the radial expansion of plasma perpendicular to the nanotubes rather than to the front side. These features may contribute to fast ignition in inertial confinement fusion and laser particle acceleration, both of which require high current and small surface plasma simultaneously.

Self-Consistency of Rain Event Definitions

NASA Astrophysics Data System (ADS)

Teves, J. B.; Larsen, M.

2014-12-01

A dense optical rain disdrometer array was constructed to study rain variability on spatial scales less than 100 meters with temporal resolution of 1 minute. Approximately two months of data were classified into rain events using methods common in the literature. These methods were unable to identify an array-wide consensus as to the total number of rain events; instruments as little as 2 meters apart with similar data records sometimes identified different rain event totals. Physical considerations suggest that these differing event totals are likely due to instrument sampling fluctuations that are typically not accounted for in rain event studies. Detection of varying numbers of rain events impact many commonly used storm statistics including storm duration distributions and mean rain rate. A summary of the results above and their implications are presented.

Enhanced biomimic bactericidal surfaces by coating with positively-charged ZIF nano-dagger arrays.

PubMed

Yuan, Yuan; Zhang, Yugen

2017-10-01

Cicada wing surfaces are covered with dense patterns of nano-pillar structure that prevent bacterial growth by rupturing adhered microbial cells. To mimic the natural nano-pillar structure, we developed a general and simple method to grow metal organic framework (MOF) nano-dagger arrays on a wide range of surfaces. These nano-daggers possess high bactericidal activity, with log reduction >7 for Escherichia coli and Staphylococcus aureus. It was hypothesized that the positively-charged ZIF-L nano-dagger surfaces enhance bacterial cell adhesion, facilitating selective and efficient bacteria killing by the rigid and sharp nano-dagger tips. This research provides a safe and clean antimicrobial surface technology which does not require external chemicals and will not cause drug resistance. Copyright © 2017 Elsevier Inc. All rights reserved.

Miniature micromachined quadrupole mass spectrometer array and method of making the same

NASA Technical Reports Server (NTRS)

Chutjian, Ara (Inventor); Brennen, Reid A. (Inventor); Hecht, Michael (Inventor); Wiberg, Dean (Inventor); Orient, Otto (Inventor)

2001-01-01

The present invention provides a quadrupole mass spectrometer and an ion filter for use in the quadrupole mass spectrometer. The ion filter includes a thin patterned layer including a two-dimensional array of poles forming one or more quadrupoles. The patterned layer design permits the use of very short poles and with a very dense spacing of the poles, so that the ion filter may be made very small. Also provided is a method for making the ion filter and the quadrupole mass spectrometer. The method involves forming the patterned layer of the ion filter in such a way that as the poles of the patterned layer are formed, they have the relative positioning and alignment for use in a final quadrupole mass spectrometer device.

Miniature micromachined quadrupole mass spectrometer array and method of making the same

NASA Technical Reports Server (NTRS)

Hecht, Michael (Inventor); Wiberg, Dean (Inventor); Orient, Otto (Inventor); Brennen, Reid A. (Inventor); Chutjian, Ara (Inventor)

2001-01-01

The present invention provides a quadrupole mass spectrometer and an ion filter for use in the quadrupole mass spectrometer. The ion filter includes a thin patterned layer including a two-dimensional array of poles forming one or more quadrupoles. The patterned layer design permits the use of very short poles and with a very dense spacing of the poles, so that the ion filter may be made very small. Also provided is a method for making the ion filter and the quadrupole mass spectrometer. The method involves forming the patterned layer of the ion filter in such a way that as the poles of the patterned layer are formed, they have the relative positioning and aligrnent for use in a final quadrupole mass spectrometer device.

Miniature micromachined quadrupole mass spectrometer array and method of making the same

NASA Technical Reports Server (NTRS)

Orient, Otto (Inventor); Wiberg, Dean (Inventor); Brennen, Reid A. (Inventor); Hecht, Michael (Inventor); Chutjian, Ara (Inventor)

2000-01-01

The present invention provides a quadrupole mass spectrometer and an ion filter for use in the quadrupole mass spectrometer. The ion filter includes a thin patterned layer including a two-dimensional array of poles forming one or more quadrupoles. The patterned layer design permits the use of very short poles and with a very dense spacing of the poles, so that the ion filter may be made very small. Also provided is a method for making the ion filter and the quadrupole mass spectrometer. The method involves forming the patterned layer of the ion filter in such a way that as the poles of the patterned layer are formed, they have the relative positioning and alignment for use in a final quadrupole mass spectrometer device.

Advanced ACTPol Multichroic Polarimeter Array Fabrication Process for 150 mm Wafers

NASA Astrophysics Data System (ADS)

Duff, S. M.; Austermann, J.; Beall, J. A.; Becker, D.; Datta, R.; Gallardo, P. A.; Henderson, S. W.; Hilton, G. C.; Ho, S. P.; Hubmayr, J.; Koopman, B. J.; Li, D.; McMahon, J.; Nati, F.; Niemack, M. D.; Pappas, C. G.; Salatino, M.; Schmitt, B. L.; Simon, S. M.; Staggs, S. T.; Stevens, J. R.; Van Lanen, J.; Vavagiakis, E. M.; Ward, J. T.; Wollack, E. J.

2016-08-01

Advanced ACTPol (AdvACT) is a third-generation cosmic microwave background receiver to be deployed in 2016 on the Atacama Cosmology Telescope (ACT). Spanning five frequency bands from 25 to 280 GHz and having just over 5600 transition-edge sensor (TES) bolometers, this receiver will exhibit increased sensitivity and mapping speed compared to previously fielded ACT instruments. This paper presents the fabrication processes developed by NIST to scale to large arrays of feedhorn-coupled multichroic AlMn-based TES polarimeters on 150-mm diameter wafers. In addition to describing the streamlined fabrication process which enables high yields of densely packed detectors across larger wafers, we report the details of process improvements for sensor (AlMn) and insulator (SiN_x) materials and microwave structures, and the resulting performance improvements.

Advanced ACTPol Multichroic Polarimeter Array Fabrication Process for 150 mm Wafers

NASA Technical Reports Server (NTRS)

Duff, S. M.; Austermann, J.; Beall, J. A.; Becker, D.; Datta, R.; Gallardo, P. A.; Henderson, S. W.; Hilton, G. C.; Ho, S. P.; Hubmayr, J.;

Distributed Fiber Optic Sensors for Earthquake Detection and Early Warning

NASA Astrophysics Data System (ADS)

Karrenbach, M. H.; Cole, S.

2016-12-01

Fiber optic cables placed along pipelines, roads or other infrastructure provide dense sampling of passing seismic wavefields. Laser interrogation units illuminate the fiber over its entire length, and strain at desired points along the fiber can be determined from the reflected signal. Single-mode optical fibers up to 50 km in length can provide a distributed acoustic sensing system (DAS) where the acoustic bandwidth of each channel is limited only by the round-trip time over the length of the cable (0.0005 s for a 50 km cable). Using a 10 m spatial resolution results in 4000 channels sampled at 2.5 kHz spanning a 40 km-long fiber deployed along a pipeline. The inline strain field is averaged along the fiber over a 10 m section of the cable at each desired spatial sample, creating a virtual sensor location. Typically, a dynamic strain sensitivity of sub-nanometers within each gauge along the entire length of the fiber can be achieved. This sensitivity corresponds to a particle displacement figure of approximately -90 dB ms-2Hz-½. Such a fiber optic sensor is not as sensitive as long-period seismometers used in earthquake networks, but given the large number of channels, small to medium-sized earthquakes can be detected, depending on distance from the array, and can be located with precision through arrival time inversions. We show several examples of earthquake recordings using distributed fiber optic arrays that were deployed originally for other purposes. A 480 km long section of a pipeline in Turkey was actively monitored with a DAS fiber optic system for activities in the immediate vicinity of the pipeline. The densely spaced sensor array along the pipeline detected earthquakes of 3.6 - 7.2 magnitude range, centered near Van, Turkey. Secondly, a fiber optic system located along a rail line near the Salton Sea in California was used to create a smaller scale fiber optic sensor array, on which earthquakes with magnitudes 2.2 - 2.7 were recorded from epicenters up to 65 km away. Our analysis shows that existing fiber optic installations along infrastructure could be combined to form a large aperture array with tens of thousands of channels for epicenter estimation and for early warning purposes, augmenting existing earthquake sensor networks.

Ge/Si(001) heterostructures with dense arrays of Ge quantum dots: morphology, defects, photo-emf spectra and terahertz conductivity

PubMed Central

2012-01-01

Issues of Ge hut cluster array formation and growth at low temperatures on the Ge/Si(001) wetting layer are discussed on the basis of explorations performed by high resolution STM and in-situ RHEED. Dynamics of the RHEED patterns in the process of Ge hut array formation is investigated at low and high temperatures of Ge deposition. Different dynamics of RHEED patterns during the deposition of Ge atoms in different growth modes is observed, which reflects the difference in adatom mobility and their ‘condensation’ fluxes from Ge 2D gas on the surface for different modes, which in turn control the nucleation rates and densities of Ge clusters. Data of HRTEM studies of multilayer Ge/Si heterostructures are presented with the focus on low-temperature formation of perfect films. Heteroepitaxial Si p–i–n-diodes with multilayer stacks of Ge/Si(001) quantum dot dense arrays built in intrinsic domains have been investigated and found to exhibit the photo-emf in a wide spectral range from 0.8 to 5 μm. An effect of wide-band irradiation by infrared light on the photo-emf spectra has been observed. Photo-emf in different spectral ranges has been found to be differently affected by the wide-band irradiation. A significant increase in photo-emf is observed in the fundamental absorption range under the wide-band irradiation. The observed phenomena are explained in terms of positive and neutral charge states of the quantum dot layers and the Coulomb potential of the quantum dot ensemble. A new design of quantum dot infrared photodetectors is proposed. By using a coherent source spectrometer, first measurements of terahertz dynamical conductivity (absorptivity) spectra of Ge/Si(001) heterostructures were performed at frequencies ranged from 0.3 to 1.2 THz in the temperature interval from 300 to 5 K. The effective dynamical conductivity of the heterostructures with Ge quantum dots has been discovered to be significantly higher than that of the structure with the same amount of bulk germanium (not organized in an array of quantum dots). The excess conductivity is not observed in the structures with the Ge coverage less than 8 Å. When a Ge/Si(001) sample is cooled down the conductivity of the heterostructure decreases. PMID:22824144

Ge/Si(001) heterostructures with dense arrays of Ge quantum dots: morphology, defects, photo-emf spectra and terahertz conductivity.

PubMed

Yuryev, Vladimir A; Arapkina, Larisa V; Storozhevykh, Mikhail S; Chapnin, Valery A; Chizh, Kirill V; Uvarov, Oleg V; Kalinushkin, Victor P; Zhukova, Elena S; Prokhorov, Anatoly S; Spektor, Igor E; Gorshunov, Boris P

2012-07-23

: Issues of Ge hut cluster array formation and growth at low temperatures on the Ge/Si(001) wetting layer are discussed on the basis of explorations performed by high resolution STM and in-situ RHEED. Dynamics of the RHEED patterns in the process of Ge hut array formation is investigated at low and high temperatures of Ge deposition. Different dynamics of RHEED patterns during the deposition of Ge atoms in different growth modes is observed, which reflects the difference in adatom mobility and their 'condensation' fluxes from Ge 2D gas on the surface for different modes, which in turn control the nucleation rates and densities of Ge clusters. Data of HRTEM studies of multilayer Ge/Si heterostructures are presented with the focus on low-temperature formation of perfect films.Heteroepitaxial Si p-i-n-diodes with multilayer stacks of Ge/Si(001) quantum dot dense arrays built in intrinsic domains have been investigated and found to exhibit the photo-emf in a wide spectral range from 0.8 to 5 μm. An effect of wide-band irradiation by infrared light on the photo-emf spectra has been observed. Photo-emf in different spectral ranges has been found to be differently affected by the wide-band irradiation. A significant increase in photo-emf is observed in the fundamental absorption range under the wide-band irradiation. The observed phenomena are explained in terms of positive and neutral charge states of the quantum dot layers and the Coulomb potential of the quantum dot ensemble. A new design of quantum dot infrared photodetectors is proposed.By using a coherent source spectrometer, first measurements of terahertz dynamical conductivity (absorptivity) spectra of Ge/Si(001) heterostructures were performed at frequencies ranged from 0.3 to 1.2 THz in the temperature interval from 300 to 5 K. The effective dynamical conductivity of the heterostructures with Ge quantum dots has been discovered to be significantly higher than that of the structure with the same amount of bulk germanium (not organized in an array of quantum dots). The excess conductivity is not observed in the structures with the Ge coverage less than 8 Å. When a Ge/Si(001) sample is cooled down the conductivity of the heterostructure decreases.

Matching native electrical stimulation by graded chemical stimulation in isolated mouse adrenal chromaffin cells.

PubMed

Fulop, Tiberiu; Smith, Corey

2007-11-30

Adrenal chromaffin cells release multiple transmitters in response to sympathetic stimulation. Modest cell firing, matching sympathetic tone, releases small freely soluble catecholamines. Elevated electrical firing rates matching input under sympathetic stress results in release of catecholamines as well as semi-soluble vaso- and neuro-active peptides packaged within the dense core of the secretory granule. This activity-dependent differential transmitter release has been shown to rely on a mechanistic shift in the mode of exocytosis through the regulated dilation of the secretory fusion pore between granule and cell surface membranes. However, biochemical description of the mechanism regulating fusion pore dilation remains elusive. In the experimental setting, electrical stimulation designed to mimic sympathetic input, is achieved through single-cell voltage-clamp. While precise, this approach is incompatible with biochemical and proteomic analysis, both of which require large sample sizes. We address this limitation in the current study. We describe a bulk chemical stimulation paradigm calibrated to match defined electrical activity. We utilize calcium and single-cell amperometric measurements to match extracellular potassium concentrations to physiological electrical stimulation under sympathetic tone as well as acute stress conditions. This approach provides larger samples of uniformly stimulated cells for determining molecular players in activity-dependent differential transmitter release from adrenal chromaffin cells.

Artificial retina model for the retinally blind based on wavelet transform

NASA Astrophysics Data System (ADS)

Zeng, Yan-an; Song, Xin-qiang; Jiang, Fa-gang; Chang, Da-ding

2007-01-01

Artificial retina is aimed for the stimulation of remained retinal neurons in the patients with degenerated photoreceptors. Microelectrode arrays have been developed for this as a part of stimulator. Design such microelectrode arrays first requires a suitable mathematical method for human retinal information processing. In this paper, a flexible and adjustable human visual information extracting model is presented, which is based on the wavelet transform. With the flexible of wavelet transform to image information processing and the consistent to human visual information extracting, wavelet transform theory is applied to the artificial retina model for the retinally blind. The response of the model to synthetic image is shown. The simulated experiment demonstrates that the model behaves in a manner qualitatively similar to biological retinas and thus may serve as a basis for the development of an artificial retina.

Direct Intracochlear Acoustic Stimulation Using a PZT Microactuator

PubMed Central

Luo, Chuan; Omelchenko, Irina; Manson, Robert; Robbins, Carol; Oesterle, Elizabeth C.; Cao, Guo Zhong; Hume, Clifford R.

2015-01-01

Combined electric and acoustic stimulation has proven to be an effective strategy to improve hearing in some cochlear implant users. We describe an acoustic microactuator to directly deliver stimuli to the perilymph in the scala tympani. The 800 µm by 800 µm actuator has a silicon diaphragm driven by a piezoelectric thin film (e.g., lead-zirconium-titanium oxide or PZT). This device could also be used as a component of a bimodal acoustic-electric electrode array. In the current study, we established a guinea pig model to test the actuator for its ability to deliver auditory signals to the cochlea in vivo. The actuator was placed through the round window of the cochlea. Auditory brainstem response (ABR) thresholds, peak latencies, and amplitude growth were calculated for an ear canal speaker versus the intracochlear actuator for tone burst stimuli at 4, 8, 16, and 24 kHz. An ABR was obtained after removal of the probe to assess loss of hearing related to the procedure. In some animals, the temporal bone was harvested for histologic analysis of cochlear damage. We show that the device is capable of stimulating ABRs in vivo with latencies and growth functions comparable to stimulation in the ear canal. Further experiments will be necessary to evaluate the efficiency and safety of this modality in long-term auditory stimulation and its ability to be integrated with conventional cochlear implant arrays. PMID:26631107

Optical responses evoked by white matter stimulation in rat visual cortical slices and their relation to neural activities.

PubMed

Tanifuji, M; Yamanaka, A; Sunaba, R; Terakawa, S; Toyama, K

1996-10-28

To characterize optical responses (ORs) evoked by white matter (WM) stimulation in slices of rat visual cortex (VC) stained with voltage sensitive dyes, time course of ORs in each layer was investigated by recording ORs with a linearly aligned photodiode array, and the spatial patterns of the ORs at specified time after stimulation were investigated by a CCD camera in combination with stroboscopic illumination. The ORs recorded by the photodiode array were an increase in absorption at 700 nm and a decrease in the wavelength below 650 nm, suggesting that the ORs were dye related. The ORs were compared with field potentials (FPs) to clarify that neural events were represented by the ORs, and in support of this view, we found that the first order spatial differentials of ORs and that of FPs were in good agreement. We further compared ORs with intracellular responses, and found that the ORs mainly represent postsynaptic potentials (PSPs) of VC neurons except for the deeper part of layer VI, where a component representing action potentials in fibers stimulated directly was observed. The time-lapse imaging of ORs showed that excitation first propagated vertically up to layer I and subsequently in the horizontal direction along layers II-III and V-VI as in previous investigations. Spatio-temporal patterns of ORs under blockade of synaptic transmission were also investigated to reveal activity of fibers evoked by WM stimulation which produced such patterns of propagation.

Electron Beam "Writes" Silicon On Sapphire

NASA Technical Reports Server (NTRS)

Heinemann, Klaus

1988-01-01

Method of growing silicon on sapphire substrate uses beam of electrons to aid growth of semiconductor material. Silicon forms as epitaxial film in precisely localized areas in micron-wide lines. Promising fabrication method for fast, densely-packed integrated circuits. Silicon deposited preferentially in contaminated substrate zones and in clean zone irradiated by electron beam. Electron beam, like surface contamination, appears to stimulate decomposition of silane atmosphere.

Flat-plate solar array project of the US Department of Energy's National Photovoltaics Program: Ten years of progress

NASA Technical Reports Server (NTRS)

Christensen, Elmer

1985-01-01

The Flat-Plate Solar Array (FSA) Project, a Government-sponsored photovoltaics project, was initiated in January 1975 (previously named the Low-Cost Silicon Solar Array Project) to stimulate the development of PV systems for widespread use. Its goal then was to develop PV modules with 10% efficiency, a 20-year lifetime, and a selling price of $0.50 per peak watt of generating capacity (1975 dollars). It was recognized that cost reduction of PV solar-cell and module manufacturing was the key achievement needed if PV power systems were to be economically competitive for large-scale terrestrial use.

An inverter/controller subsystem optimized for photovoltaic applications

NASA Technical Reports Server (NTRS)

Pickrell, R. L.; Osullivan, G.; Merrill, W. C.

1978-01-01

Conversion of solar array dc power to ac power stimulated the specification, design, and simulation testing of an inverter/controller subsystem tailored to the photovoltaic power source characteristics. Optimization of the inverter/controller design is discussed as part of an overall photovoltaic power system designed for maximum energy extraction from the solar array. The special design requirements for the inverter/ controller include: a power system controller (PSC) to control continuously the solar array operating point at the maximum power level based on variable solar insolation and cell temperatures; and an inverter designed for high efficiency at rated load and low losses at light loadings to conserve energy.

Statistical analysis of modal properties of a cable-stayed bridge through long-term structural health monitoring with wireless smart sensor networks

NASA Astrophysics Data System (ADS)

Asadollahi, Parisa; Li, Jian

2016-04-01

Understanding the dynamic behavior of complex structures such as long-span bridges requires dense deployment of sensors. Traditional wired sensor systems are generally expensive and time-consuming to install due to cabling. With wireless communication and on-board computation capabilities, wireless smart sensor networks have the advantages of being low cost, easy to deploy and maintain and therefore facilitate dense instrumentation for structural health monitoring. A long-term monitoring project was recently carried out for a cable-stayed bridge in South Korea with a dense array of 113 smart sensors, which feature the world's largest wireless smart sensor network for civil structural monitoring. This paper presents a comprehensive statistical analysis of the modal properties including natural frequencies, damping ratios and mode shapes of the monitored cable-stayed bridge. Data analyzed in this paper is composed of structural vibration signals monitored during a 12-month period under ambient excitations. The correlation between environmental temperature and the modal frequencies is also investigated. The results showed the long-term statistical structural behavior of the bridge, which serves as the basis for Bayesian statistical updating for the numerical model.

Development of a spectroscopic technique for simultaneous magnetic field, electron density, and temperature measurements in ICF-relevant plasmas.

PubMed

Dutra, E C; Koch, J A; Presura, R; Angermeier, W A; Darling, T; Haque, S; Mancini, R C; Covington, A M

2016-11-01

Spectroscopic techniques in the visible range are often used in plasma experiments to measure B-field induced Zeeman splitting, electron densities via Stark broadening, and temperatures from Doppler broadening. However, when electron densities and temperatures are sufficiently high, the broadening of the Stark and Doppler components can dominate the emission spectra and obscure the Zeeman component. In this research, we are developing a time-resolved multi-axial technique for measuring the Zeeman, Stark, and Doppler broadened line emission of dense magnetized plasmas for Z-pinch and Dense Plasma Focus (DPF) accelerators. The line emission is used to calculate the electron densities, temperatures, and B-fields. In parallel, we are developing a line-shape modeling code that incorporates the broadening effects due to Stark, Doppler, and Zeeman effects for dense magnetized plasma. This manuscript presents the details of the experimental setup and line shape code, along with the results obtained from an Al iii doublet at the University of Nevada, Reno at Nevada Terawatt Facility. Future tests are planned to further evaluate the technique and modeling on other material wire array, gas puff, and DPF platforms.

Environmental change drives accelerated adaptation through stimulated copy number variation

PubMed Central

Hull, Ryan M.; Cruz, Cristina; Jack, Carmen V.

2017-01-01

Copy number variation (CNV) is rife in eukaryotic genomes and has been implicated in many human disorders, particularly cancer, in which CNV promotes both tumorigenesis and chemotherapy resistance. CNVs are considered random mutations but often arise through replication defects; transcription can interfere with replication fork progression and stability, leading to increased mutation rates at highly transcribed loci. Here we investigate whether inducible promoters can stimulate CNV to yield reproducible, environment-specific genetic changes. We propose a general mechanism for environmentally-stimulated CNV and validate this mechanism for the emergence of copper resistance in budding yeast. By analysing a large cohort of individual cells, we directly demonstrate that CNV of the copper-resistance gene CUP1 is stimulated by environmental copper. CNV stimulation accelerates the formation of novel alleles conferring enhanced copper resistance, such that copper exposure actively drives adaptation to copper-rich environments. Furthermore, quantification of CNV in individual cells reveals remarkable allele selectivity in the rate at which specific environments stimulate CNV. We define the key mechanistic elements underlying this selectivity, demonstrating that CNV is regulated by both promoter activity and acetylation of histone H3 lysine 56 (H3K56ac) and that H3K56ac is required for CUP1 CNV and efficient copper adaptation. Stimulated CNV is not limited to high-copy CUP1 repeat arrays, as we find that H3K56ac also regulates CNV in 3 copy arrays of CUP1 or SFA1 genes. The impact of transcription on DNA damage is well understood, but our research reveals that this apparently problematic association forms a pathway by which mutations can be directed to particular loci in particular environments and furthermore that this mutagenic process can be regulated through histone acetylation. Stimulated CNV therefore represents an unanticipated and remarkably controllable pathway facilitating organismal adaptation to new environments. PMID:28654659

Screening for Selective Protein Inhibitors by Using the IANUS Peptide Array.

PubMed

Erdmann, Frank; Prell, Erik; Jahreis, Günther; Fischer, Gunter; Malešević, Miroslav

2018-04-16

Finding new road blacks: A peptidic inhibitor of calcineurin (CaN)-mediated nuclear factor of activated T cells (NFAT) dephosphorylation, which is developed through a template-assisted IANUS (Induced orgANisation of strUcture by matrix-assisted togethernesS) peptide array, is cell permeable and able to block the translocation of green fluorescent protein-NFAT fusion protein (GFP-NFAT) into the nucleus after stimulation. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Non-invasive method for selection of electrodes and stimulus parameters for FES applications with intrafascicular arrays

NASA Astrophysics Data System (ADS)

Dowden, B. R.; Frankel, M. A.; Normann, R. A.; Clark, G. A.

2012-02-01

High-channel-count intrafascicular electrode arrays provide comprehensive and selective access to the peripheral nervous system. One practical difficulty in using several electrode arrays to evoke coordinated movements in paralyzed limbs is the identification of the appropriate stimulation channels and stimulus parameters to evoke desired movements. Here we present the use of a six degree-of-freedom load cell placed under the foot of a feline to characterize the muscle activation produced by three 100-electrode Utah Slanted Electrode Arrays (USEAs) implanted into the femoral nerves, sciatic nerves, and muscular branches of the sciatic nerves of three cats. Intramuscular stimulation was used to identify the endpoint force directions produced by 15 muscles of the hind limb, and these directions were used to classify the forces produced by each intrafascicular USEA electrode as flexion or extension. For 451 USEA electrodes, stimulus intensities for threshold and saturation muscle forces were identified, and the 3D direction and linearity of the force recruitment curves were determined. Further, motor unit excitation independence for 198 electrode pairs was measured using the refractory technique. This study demonstrates the utility of 3D endpoint force monitoring as a simple and non-invasive metric for characterizing the muscle-activation properties of hundreds of implanted peripheral nerve electrodes, allowing for electrode and parameter selection for neuroprosthetic applications.

Non-invasive method for selection of electrodes and stimulus parameters for FES applications with intrafascicular arrays.

PubMed

Dowden, B R; Frankel, M A; Normann, R A; Clark, G A

2012-02-01

High-channel-count intrafascicular electrode arrays provide comprehensive and selective access to the peripheral nervous system. One practical difficulty in using several electrode arrays to evoke coordinated movements in paralyzed limbs is the identification of the appropriate stimulation channels and stimulus parameters to evoke desired movements. Here we present the use of a six degree-of-freedom load cell placed under the foot of a feline to characterize the muscle activation produced by three 100-electrode Utah Slanted Electrode Arrays (USEAs) implanted into the femoral nerves, sciatic nerves, and muscular branches of the sciatic nerves of three cats. Intramuscular stimulation was used to identify the endpoint force directions produced by 15 muscles of the hind limb, and these directions were used to classify the forces produced by each intrafascicular USEA electrode as flexion or extension. For 451 USEA electrodes, stimulus intensities for threshold and saturation muscle forces were identified, and the 3D direction and linearity of the force recruitment curves were determined. Further, motor unit excitation independence for 198 electrode pairs was measured using the refractory technique. This study demonstrates the utility of 3D endpoint force monitoring as a simple and non-invasive metric for characterizing the muscle-activation properties of hundreds of implanted peripheral nerve electrodes, allowing for electrode and parameter selection for neuroprosthetic applications.

A Brief Update on the CMZoom Survey

NASA Astrophysics Data System (ADS)

Battersby, C.; Keto, E.; Zhang, Q.; Longmore, S. N.; Kruijssen, J. M. D.; Pillai, T.; Kauffmann, J.; Walker, D.; Lu, X.; Ginsburg, A.; Bally, J.; Mills, E. A. C.; Henshaw, J.; Immer, K.; Patel, N.; Tolls, V.; Walsh, A.; Johnston, K.; Ho, L. C.

2017-01-01

The inner few hundred parsecs of the Milky Way, the Central Molecular Zone (CMZ), is our closest laboratory for understanding star formation in the extreme environments (hot, dense, turbulent gas) that once dominated the universe. We present an update on the first large-area survey to expose the sites of star formation across the CMZ at high-resolution in submillimeter wavelengths: the CMZoom survey with the Submillimeter Array (SMA). We identify the locations of dense cores and search for signatures of embedded star formation. CMZoom is a three-year survey in its final year and is mapping out the highest column density regions of the CMZ in dust continuum and a variety of spectral lines around 1.3 mm. CMZoom combines SMA compact and subcompact configurations with single-dish data from BGPS and the APEX telescope, achieving an angular resolution of about 4'' (0.2 pc) and good image fidelity up to large spatial scales.

Nanostructured ZnO films with various morphologies prepared by ultrasonic spray pyrolysis and its growing process

NASA Astrophysics Data System (ADS)

Ma, H. L.; Liu, Z. W.; Zeng, D. C.; Zhong, M. L.; Yu, H. Y.; Mikmekova, E.

2013-10-01

Nanostructured ZnO films were prepared by the ultrasonic spray pyrolysis method using Zn(CH3COO)2·2H2O as a precursor. The effects of substrate temperature (Ts) on the morphology and properties were systematically studied. As the Ts increased from 430 °C to 610 °C, the morphology of the film transforms from closed packed nanosheets to dense nanocrystalline film and then to hexagonal nanorod array. The dense film formed at a temperature of 550 °C has the lowest electric resistivity and highest carrier concentration. The optical transmittance for all prepared samples was higher than 90%. The photoluminescence (PL) properties varied with the Ts due to the internal defect difference. The growth mechanism of ZnO film involves island growth and diffusion, which was evident by observing the samples prepared at various times.

From ATLASGAL to SEDIGISM: Towards a Complete 3D View of the Dense Galactic Interstellar Medium

NASA Astrophysics Data System (ADS)

Schuller, F.; Urquhart, J.; Bronfman, L.; Csengeri, T.; Bontemps, S.; Duarte-Cabral, A.; Giannetti, A.; Ginsburg, A.; Henning, T.; Immer, K.; Leurini, S.; Mattern, M.; Menten, K.; Molinari, S.; Muller, E.; Sánchez-Monge, A.; Schisano, E.; Suri, S.; Testi, L.; Wang, K.; Wyrowski, F.; Zavagno, A.

2016-09-01

The ATLASGAL survey has provided the first unbiased view of the inner Galactic Plane at sub-millimetre wavelengths. This is the largest ground-based survey of its kind to date, covering 420 square degrees at a wavelength of 870 µm. The reduced data, consisting of images and a catalogue of > 104 compact sources, are available from the ESO Science Archive Facility through the Phase 3 infrastructure. The extremely rich statistics of this survey initiated several follow-up projects, including spectroscopic observations to explore molecular complexity and high angular resolution imaging with the Atacama Large Millimeter/submillimeter Array (ALMA), aimed at resolving individual protostars. The most extensive follow-up project is SEDIGISM, a 3D mapping of the dense interstellar medium over a large fraction of the inner Galaxy. Some notable results of these surveys are highlighted.

Behavioral assessment of sensitivity to intracortical microstimulation of primate somatosensory cortex.

PubMed

Kim, Sungshin; Callier, Thierri; Tabot, Gregg A; Gaunt, Robert A; Tenore, Francesco V; Bensmaia, Sliman J

2015-12-08

Intracortical microstimulation (ICMS) is a powerful tool to investigate the functional role of neural circuits and may provide a means to restore sensation for patients for whom peripheral stimulation is not an option. In a series of psychophysical experiments with nonhuman primates, we investigate how stimulation parameters affect behavioral sensitivity to ICMS. Specifically, we deliver ICMS to primary somatosensory cortex through chronically implanted electrode arrays across a wide range of stimulation regimes. First, we investigate how the detectability of ICMS depends on stimulation parameters, including pulse width, frequency, amplitude, and pulse train duration. Then, we characterize the degree to which ICMS pulse trains that differ in amplitude lead to discriminable percepts across the range of perceptible and safe amplitudes. We also investigate how discriminability of pulse amplitude is modulated by other stimulation parameters-namely, frequency and duration. Perceptual judgments obtained across these various conditions will inform the design of stimulation regimes for neuroscience and neuroengineering applications.

Ge quantum dot arrays grown by ultrahigh vacuum molecular-beam epitaxy on the Si(001) surface: nucleation, morphology, and CMOS compatibility

PubMed Central

2011-01-01

Issues of morphology, nucleation, and growth of Ge cluster arrays deposited by ultrahigh vacuum molecular beam epitaxy on the Si(001) surface are considered. Difference in nucleation of quantum dots during Ge deposition at low (≲600°C) and high (≳600°C) temperatures is studied by high resolution scanning tunneling microscopy. The atomic models of growth of both species of Ge huts--pyramids and wedges-- are proposed. The growth cycle of Ge QD arrays at low temperatures is explored. A problem of lowering of the array formation temperature is discussed with the focus on CMOS compatibility of the entire process; a special attention is paid upon approaches to reduction of treatment temperature during the Si(001) surface pre-growth cleaning, which is at once a key and the highest-temperature phase of the Ge/Si(001) quantum dot dense array formation process. The temperature of the Si clean surface preparation, the final high-temperature step of which is, as a rule, carried out directly in the MBE chamber just before the structure deposition, determines the compatibility of formation process of Ge-QD-array based devices with the CMOS manufacturing cycle. Silicon surface hydrogenation at the final stage of its wet chemical etching during the preliminary cleaning is proposed as a possible way of efficient reduction of the Si wafer pre-growth annealing temperature. PMID:21892938

A compact integrated device for spatially selective optogenetic neural stimulation based on the Utah Optrode Array

NASA Astrophysics Data System (ADS)

Scharf, Robert; Reiche, Christopher F.; McAlinden, Niall; Cheng, Yunzhou; Xie, Enyuan; Sharma, Rohit; Tathireddy, Prashant; Rieth, Loren; Mathieson, Keith; Blair, Steve

2018-02-01

Optogenetics is a powerful tool for neural control, but controlled light delivery beyond the superficial structures of the brain remains a challenge. For this, we have developed an optrode array, which can be used for optogenetic stimulation of the deep layers of the cortex. The device consists of a 10×10 array of penetrating optical waveguides, which are predefined using BOROFLOAT® wafer dicing. A wet etch step is then used to achieve the desired final optrode dimensions, followed by heat treatment to smoothen the edges and the surface. The major challenge that we have addressed is delivering light through individual waveguides in a controlled and efficient fashion. Simply coupling the waveguides in the optrode array to a separately-fabricated μLED array leads to low coupling efficiency and significant light scattering in the optrode backplane and crosstalk to adjacent optrodes due to the large mismatch between the μLED and waveguide numerical aperture and the working distance between them. We mitigate stray light by reducing the thickness of the glass backplane and adding a silicon interposer layer with optical vias connecting the μLEDs to the optrodes. The interposer additionally provides mechanical stability required by very thin backplanes, while restricting the unwanted spread of light. Initial testing of light output from the optrodes confirms intensity levels sufficient for optogenetic neural activation. These results pave the way for future work, which will focus on optimization of light coupling and adding recording electrodes to each optrode shank to create a bidirectional optoelectronic interface.

Virtual electrodes for high-density electrode arrays

DOEpatents

Cela, Carlos J.; Lazzi, Gianluca

2015-10-13

The present embodiments are directed to implantable electrode arrays having virtual electrodes. The virtual electrodes may improve the resolution of the implantable electrode array without the burden of corresponding complexity of electronic circuitry and wiring. In a particular embodiment, a virtual electrode may include one or more passive elements to help steer current to a specific location between the active electrodes. For example, a passive element may be a metalized layer on a substrate that is adjacent to, but not directly connected to an active electrode. In certain embodiments, an active electrode may be directly coupled to a power source via a conductive connection. Beneficially, the passive elements may help to increase the overall resolution of the implantable array by providing additional stimulation points without requiring additional wiring or driver circuitry for the passive elements.

Densely Aligned Graphene Nanoribbon Arrays and Bandgap Engineering

DOE Office of Scientific and Technical Information (OSTI.GOV)

Su, Justin; Chen, Changxin; Gong, Ming

Graphene has attracted great interest for future electronics due to its high mobility and high thermal conductivity. However, a two-dimensional graphene sheet behaves like a metal, lacking a bandgap needed for the key devices components such as field effect transistors (FETs) in digital electronics. It has been shown that, partly due to quantum confinement, graphene nanoribbons (GNRs) with ~2 nm width can open up sufficient bandgaps and evolve into semiconductors to exhibit high on/off ratios useful for FETs. However, a challenging problem has been that, such ultra-narrow GNRs (~2 nm) are difficult to fabricate, especially for GNRs with smooth edgesmore » throughout the ribbon length. Despite high on/off ratios, these GNRs show very low mobility and low on-state conductance due to dominant scattering effects by imperfections and disorders at the edges. Wider GNRs (>5 nm) show higher mobility, higher conductance but smaller bandgaps and low on/off ratios undesirable for FET applications. It is highly desirable to open up bandgaps in graphene or increase the bandgaps in wide GNRs to afford graphene based semiconductors for high performance (high on-state current and high on/off ratio) electronics. Large scale ordering and dense packing of such GNRs in parallel are also needed for device integration but have also been challenging thus far. It has been shown theoretically that uniaxial strains can be applied to a GNR to engineer its bandgap. The underlying physics is that under uniaxial strain, the Dirac point moves due to stretched C-C bonds, leading to an increase in the bandgap of armchair GNRs by up to 50% of its original bandgap (i.e. bandgap at zero strain). For zigzag GNRs, due to the existence of the edge states, changes of bandgap are smaller under uniaxial strain and can be increased by ~30%. This work proposes a novel approach to the fabrication of densely aligned graphene nanoribbons with highly smooth edges afforded by anisotropic etching and uniaxial strain for bandgap engineering of GNRs towards high on/off ratio and high on-state current GNR devices. First, we will develop a novel approach for the fabrication of high density GNR arrays (pitch <50 nm, tunable down to 30nm) with pre-defined edge orientation and smooth edges using a free standing nano-mask derived from diblock copolymer assembly for patterning of graphene sheets. Anisotropic graphene edges will be developed to afford smooth edges along crystallographic lattice directions. Then, we will fabricate GNR devices on flexible substrates and apply uniaxial strain to engineer the bandgap of the GNRs. The bandgap of GNRs could be increased by up to 50% under uniaxial strain according to theoretical calculations and will be investigated through electrical transport measurements. Micro-Raman spectroscopy of single GNRs and parallel arrays will be used to probe and quantify the uniaxial strain. Electrical measurements will be used to probe the on/off ratio of GNR FET devices and confirm the bandgap tuning effects. Finally, we plan to use dense parallel arrays of GNRs to demonstrate strained GNR field effect transistors with high on/off ratios and high on-state current, and compare strained GNR FETs with carbon nanotube and Si based field effect transistor (FET) devices.« less

Glucose recruits K(ATP) channels via non-insulin-containing dense-core granules.

PubMed

Yang, Shao-Nian; Wenna, Nancy Dekki; Yu, Jia; Yang, Guang; Qiu, Hua; Yu, Lina; Juntti-Berggren, Lisa; Köhler, Martin; Berggren, Per-Olof

2007-09-01

beta cells rely on adenosine triphosphate-sensitive potassium (K(ATP)) channels to initiate and end glucose-stimulated insulin secretion through changes in membrane potential. These channels may also act as a constituent of the exocytotic machinery to mediate insulin release independent of their electrical function. However, the molecular mechanisms whereby the beta cell plasma membrane maintains an appropriate number of K(ATP) channels are not known. We now show that glucose increases K(ATP) current amplitude by increasing the number of K(ATP) channels in the beta cell plasma membrane. The effect was blocked by inhibition of protein kinase A (PKA) as well as by depletion of extracellular or intracellular Ca(2+). Furthermore, glucose promoted recruitment of the potassium inward rectifier 6.2 to the plasma membrane, and intracellular K(ATP) channels localized in chromogranin-positive/insulin-negative dense-core granules. Our data suggest that glucose can recruit K(ATP) channels to the beta cell plasma membrane via non-insulin-containing dense-core granules in a Ca(2+)- and PKA-dependent manner.

Optical tomography of fluorophores in dense scattering media based on ultrasound-enhanced chemiluminescence

NASA Astrophysics Data System (ADS)

Kobayashi, Masaki; Kikuchi, Naoto; Sato, Akihiro

2015-01-01

This letter proposes and demonstrates ultrasound-combined optical imaging in dense scattering media. A peroxyoxalate chemiluminescence system that includes fluorophores to chemically excite the pigment is stimulated by ultrasound irradiation with power of less than 0.14 W/cm2. Using focused ultrasound, the chemiluminescence is selectively spatially enhanced, which leads to imaging of the pigment when embedded in a light-scattering medium via scanning of the focal point. The ultrasonically enhanced intensity of the chemiluminescence depends on the base intensity of the chemiluminescence without the applied ultrasound irradiation, which thereby enables quantitative determination of the fluorophore concentration. The authors demonstrate the potential of this method to resolve chemiluminescent targets in a dense scattering medium that is comparable to biological tissue. An image was acquired of a chemiluminescent target that included indocyanine green as the fluorophore embedded at a depth of 20 mm in an Intralipid-10% 200 ml/l solution scattering medium (the reduced scattering coefficient was estimated to be approximately 1.3 mm-1), indicating the potential for expansion of this technique for use in biological applications.

Munc13 homology domain-1 in CAPS/UNC31 mediates SNARE binding required for priming vesicle exocytosis.

PubMed

Khodthong, Chuenchanok; Kabachinski, Greg; James, Declan J; Martin, Thomas F J

2011-08-03

Neuropeptide and peptide hormone secretion from neural and endocrine cells occurs by Ca(2+)-triggered dense-core vesicle exocytosis. The membrane fusion machinery consisting of vesicle and plasma membrane SNARE proteins needs to be assembled for Ca(2+)-triggered vesicle exocytosis. The related Munc13 and CAPS/UNC31 proteins that prime vesicle exocytosis are proposed to promote SNARE complex assembly. CAPS binds SNARE proteins and stimulates SNARE complex formation on liposomes, but the relevance of SNARE binding to CAPS function in cells had not been determined. Here we identify a core SNARE-binding domain in CAPS as corresponding to Munc13 homology domain-1 (MHD1). CAPS lacking a single helix in MHD1 was unable to bind SNARE proteins or to support the Ca(2+)-triggered exocytosis of either docked or newly arrived dense-core vesicles. The results show that MHD1 is a SNARE-binding domain and that SNARE protein binding is essential for CAPS function in dense-core vesicle exocytosis. Copyright © 2011 Elsevier Inc. All rights reserved.

Technologies for Protein Analysis and Tissue Engineering, with Applications in Cancer

NASA Astrophysics Data System (ADS)

Vermesh, Udi Benjamin

The first part of this thesis describes electrolyte transport through an array of 20 nm wide, 20 mum long SiO2 nanofluidic transistors. At sufficiently low ionic strength, the Debye screening length exceeds the channel width, and ion transport is limited by the negatively charged channel surfaces. At source-drain biases > 5 V, the current exhibits a sharp, nonlinear increase, with a 20 - 50-fold conductance enhancement. This behavior is attributed to a breakdown of the zero-slip condition. Implications for peptide sequencing as well as energy conversion devices are discussed. The next part describes a technology for the detection of the highly aggressive brain cancer glioblastoma multiforme (GBM). In this study, we used an antibody-based microarray to compare plasma samples from glioblastoma patients and healthy controls with respect to the plasma levels of 35 different proteins known to be generally associated with tumor growth, survival, invasion, migration, and immune regulation. Average-linkage hierarchical clustering of the patient data stratified the two groups effectively, permitting accurate assignment of test samples into either GBM or healthy control groups with a sensitivity and specificity as high as 90 % and 94 %, respectively. Using the same 35-protein panel, we then analyzed plasma samples from GBM patients who were treated with the chemotherapeutic drug Avastin (Bevacizumab) and were able to effectively stratify patients based on treatment-responsiveness. Finally, single-cell resolution patterning of tissue engineered structures is demonstrated. The proper functioning of engineered constructs for tissue and organ transplantation requires positioning different cell types in anatomically precise arrangements that mimic their configurations in native tissues. Toward this end, we have developed a technique that involves two microfluidic-patterning steps run perpendicularly to each other using "anchor" and "bridge" DNA oligomers to create dense arrays of DNA grids which can then be converted into cell arrays. As a proof-of-concept, both a neuron-astrocyte construct and a pancreatic islet construct containing 2 distinct islet cell types were patterned separately as a dense array of cell grids. Once fixed in a hydrogel matrix, layers of patterned cells were then stacked to form 3-D tissue engineered constructs.

3D velocity imaging of Hikurangi subduction beneath the Wellington region, New Zealand

NASA Astrophysics Data System (ADS)

Wech, A.; Henrys, S. A.; Sutherland, R.; Seward, A. M.; Stern, T. A.; Sato, H.; Okaya, D. A.; Bassett, D.

2011-12-01

We present first results from the Seismic Array HiKurangi Experiment (SAHKE). This joint project involving institutions from New Zealand, Japan and the USA aims to investigate the subduction zone fault characteristics beneath the southernmost part of New Zealand's North Island. Situated above where the Pacific Plate is subducting beneath the Australian plate at a rate of ~42 mm/yr, the Wellington region provides a unique opportunity to investigate the frictional properties, geometry, and seismic potential of a shallow, locked megathrust fault. Here the coupled plate interface is 20-30 km deep beneath land and can be sampled with onshore-offshore data from 3 sides. An added interest to this project is that the elevated, oceanic, Hikurangi plateau has entered the subduction zone, east of Wellington, but it is still unclear how far the plateau has advanced westward into the subduction zone. SAHKE combines active and passive source data comprising 4 distinct data sets. 1) A dense temporary array of 50 seismometers with ~7 km spacing augmented 25 regional network instruments to record 49 local and 45 teleseismic earthquakes over a four month period. 2) These stations also recorded 69,000 offshore airgun shots from 17 lines crisscrossing two sides of the array. 3) An additional coast-to-coast transect of 50 stations cutting through the temporary array recorded ~2000 offshore shots on either side. 4) 1000 stations with 100m spacing along that same transect separately recorded 12 in-line, 500 kg onshore dynamite explosions. First inspection of the recent onshore shot gathers show excellent signal to noise and a band of three strong reflectors between 20 and 38 km at the western end of the profile. We combine shot and earthquake recordings to simultaneously invert ~750,000 first arrivals for velocity structure and hypocenters in the densely sampled volume. First results from 3D, Vp tomography and relocated hypocenters agree with previous studies and suggest the later weak signals are reflections from the top of the Pacific plate. Our improved velocity model provides a high-resolution geometry of the subducting plate to support interpretation of other phases identified in SAHKE shot gathers.

Site response, shallow shear-wave velocity, and wave propagation at the San Jose, California, dense seismic array

USGS Publications Warehouse

Hartzell, S.; Carver, D.; Williams, R.A.; Harmsen, S.; Zerva, A.

2003-01-01

Ground-motion records from a 52-element dense seismic array near San Jose, California, are analyzed to obtain site response, shallow shear-wave velocity, and plane-wave propagation characteristics. The array, located on the eastern side of the Santa Clara Valley south of the San Francisco Bay, is sited over the Evergreen basin, a 7-km-deep depression with Miocene and younger deposits. Site response values below 4 Hz are up to a factor of 2 greater when larger, regional records are included in the analysis, due to strong surface-wave development within the Santa Clara Valley. The pattern of site amplification is the same, however, with local or regional events. Site amplification increases away from the eastern edge of the Santa Clara Valley, reaching a maximum over the western edge of the Evergreen basin, where the pre-Cenozoic basement shallows rapidly. Amplification then decreases further to the west. This pattern may be caused by lower shallow shear-wave velocities and thicker Quaternary deposits further from the edge of the Santa Clara Valley and generation/trapping of surface waves above the shallowing basement of the western Evergreen basin. Shear-wave velocities from the inversion of site response spectra based on smaller, local earthquakes compare well with those obtained independently from our seismic reflection/refraction measurements. Velocities from the inversion of site spectra that include larger, regional records do not compare well with these measurements. A mix of local and regional events, however, is appropriate for determination of site response to be used in seismic hazard evaluation, since large damaging events would excite both body and surface waves with a wide range in ray parameters. Frequency-wavenumber, plane-wave analysis is used to determine the backazimuth and apparent velocity of coherent phases at the array. Conventional, high-resolution, and multiple signal characterization f-k power spectra and stacked slowness power spectra are compared. These spectra show surface waves generated/ scattered at the edges of the Santa Clara Valley and possibly within the valley at the western edge of the Evergreen basin.

Adapting Controlled-source Coherence Analysis to Dense Array Data in Earthquake Seismology

NASA Astrophysics Data System (ADS)

Schwarz, B.; Sigloch, K.; Nissen-Meyer, T.

2017-12-01

Exploration seismology deals with highly coherent wave fields generated by repeatable controlled sources and recorded by dense receiver arrays, whose geometry is tailored to back-scattered energy normally neglected in earthquake seismology. Owing to these favorable conditions, stacking and coherence analysis are routinely employed to suppress incoherent noise and regularize the data, thereby strongly contributing to the success of subsequent processing steps, including migration for the imaging of back-scattering interfaces or waveform tomography for the inversion of velocity structure. Attempts have been made to utilize wave field coherence on the length scales of passive-source seismology, e.g. for the imaging of transition-zone discontinuities or the core-mantle-boundary using reflected precursors. Results are however often deteriorated due to the sparse station coverage and interference of faint back-scattered with transmitted phases. USArray sampled wave fields generated by earthquake sources at an unprecedented density and similar array deployments are ongoing or planned in Alaska, the Alps and Canada. This makes the local coherence of earthquake data an increasingly valuable resource to exploit.Building on the experience in controlled-source surveys, we aim to extend the well-established concept of beam-forming to the richer toolbox that is nowadays used in seismic exploration. We suggest adapted strategies for local data coherence analysis, where summation is performed with operators that extract the local slope and curvature of wave fronts emerging at the receiver array. Besides estimating wave front properties, we demonstrate that the inherent data summation can also be used to generate virtual station responses at intermediate locations where no actual deployment was performed. Owing to the fact that stacking acts as a directional filter, interfering coherent wave fields can be efficiently separated from each other by means of coherent subtraction. We propose to construct exploration-type trace gathers, systematically investigate the potential to improve the quality and regularity of realistic synthetic earthquake data and present attempts at separating transmitted and back-scattered wave fields for the improved imaging of Earth's large-scale discontinuities.

Imaging subsurface hydrothermal structure using a dense geophone array in Yellowstone

NASA Astrophysics Data System (ADS)

Wu, S. M.; Lin, F. C.; Farrell, J.; Smith, R. B.

2016-12-01

The recent development of ambient noise cross-correlation and the availability of large N seismic arrays allow for the study of detailed shallow crustal structure. In this study, we apply multi-component noise cross-correlation to explore shallow hydrothermal structure near Old Faithful geyser in Yellowstone National Park using a temporary geophone array. The array was composed of 133 three-component 5-Hz geophones and was deployed for two weeks during November 2015. The average station spacing is 50 meters and the full aperture of the array is around 1 km with good azimuthal and spatial coverage. The Upper Geyser Basin, where Old Faithful is located, has the largest concentration of geysers in the world. This unique active hydrothermal environment and hence the extremely inhomogeneous noise source distribution makes the construction of empirical Green's functions difficult based on the traditional noise cross-correlation method. In this presentation, we show examples of the constructed cross-correlation functions and demonstrate their spatial and temporal relationships with known hydrothermal activity. We also demonstrate how useful seismic signals can be extracted from these cross-correlation functions and used for subsurface imaging. In particular, we will discuss the existence of a recharge cavity beneath Old Faithful revealed by the noise cross-correlations. In addition, we also investigated the temporal structure variation based on time-lapse noise cross-correlations and these preliminary results will also be discussed.

High-resolution microcontact printing and transfer of massive arrays of microorganisms on planar and compartmentalized nanoporous aluminium oxide.

PubMed

Ingham, Colin; Bomer, Johan; Sprenkels, Ad; van den Berg, Albert; de Vos, Willem; van Hylckama Vlieg, Johan

2010-06-07

Handling microorganisms in high throughput and their deployment into miniaturized platforms presents significant challenges. Contact printing can be used to create dense arrays of viable microorganisms. Such "living arrays", potentially with multiple identical replicates, are useful in the selection of improved industrial microorganisms, screening antimicrobials, clinical diagnostics, strain storage, and for research into microbial genetics. A high throughput method to print microorganisms at high density was devised, employing a microscope and a stamp with a massive array of PDMS pins. Viable bacteria (Lactobacillus plantarum, Esherichia coli), yeast (Candida albicans) and fungal spores (Aspergillus fumigatus) were deposited onto porous aluminium oxide (PAO) using arrays of pins with areas from 5 x 5 to 20 x 20 microm. Printing onto PAO with up to 8100 pins of 20 x 20 microm area with 3 replicates was achieved. Printing with up to 200 pins onto PAO culture chips (divided into 40 x 40 microm culture areas) allowed inoculation followed by effective segregation of microcolonies during outgrowth. Additionally, it was possible to print mixtures of C. albicans and spores of A. fumigatus with a degree of selectivity by capture onto a chemically modified PAO surface. High resolution printing of microorganisms within segregated compartments and on functionalized PAO surfaces has significant advantages over what is possible on semi-solid surfaces such as agar.

Design of hybrid two-dimensional and three-dimensional nanostructured arrays for electronic and sensing applications

NASA Astrophysics Data System (ADS)

Ko, Hyunhyub

This dissertation presents the design of organic/inorganic hybrid 2D and 3D nanostructured arrays via controlled assembly of nanoscale building blocks. Two representative nanoscale building blocks such as carbon nanotubes (one-dimension) and metal nanoparticles (zero-dimension) are the core materials for the study of solution-based assembly of nanostructured arrays. The electrical, mechanical, and optical properties of the assembled nanostructure arrays have been investigated for future device applications. We successfully demonstrated the prospective use of assembled nanostructure arrays for electronic and sensing applications by designing flexible carbon nanotube nanomembranes as mechanical sensors, highly-oriented carbon nanotubes arrays for thin-film transistors, and gold nanoparticle arrays for SERS chemical sensors. In first section, we fabricated highly ordered carbon nanotube (CNT) arrays by tilted drop-casting or dip-coating of CNT solution on silicon substrates functionalized with micropatterned self-assembled monolayers. We further exploited the electronic performance of thin-film transistors based on highly-oriented, densely packed CNT micropatterns and showed that the carrier mobility is largely improved compared to randomly oriented CNTs. The prospective use of Raman-active CNTs for potential mechanical sensors has been investigated by studying the mechano-optical properties of flexible carbon nanotube nanomembranes, which contain freely-suspended carbon nanotube array encapsulated into ultrathin (<50 nm) layer-by-layer (LbL) polymer multilayers. In second section, we fabricated 3D nano-canal arrays of porous alumina membranes decorated with gold nanoparticles for prospective SERS sensors. We showed extraordinary SERS enhancement and suggested that the high performance is associated with the combined effects of Raman-active hot spots of nanoparticle aggregates and the optical waveguide properties of nano-canals. We demonstrated the ability of this SERS substrate for trace level sensing of nitroaromatic explosives by detecting down to 100 zeptogram (˜330 molecules) of DNT.

Damage imaging using Lamb waves for SHM applications

NASA Astrophysics Data System (ADS)

Stepinski, Tadeusz; Ambroziński, Łukasz; Uhl, Tadeusz

2015-03-01

2-D ultrasonic arrays, due to their beam-steering capability and all azimuth angle coverage are a very promising tool for the inspection of plate-like structures using Lamb waves (LW). Contrary to the classical linear phased arrays (PAs) the 2D arrays enable unequivocal defect localization and they are even capable of mode selectivity of the received LWs . Recently, it has been shown that multistatic synthetic focusing (SF) algorithms applied for 2D arrays are much more effective than the classical phase array mode commonly used in NDT. The multistatic SF assumes multiple transmissions of elements in a transmitting aperture and off-line processing of the data acquired by a receiving aperture. In the simplest implementation of the technique, only a single multiplexed input and a number of output channels are required, which results in significant hardware simplification compared with the PA systems. On the one hand implementation of the multistatic SF to 2D arrays creates additional degrees of freedom during the design of the array topology, which complicates the array design process. On the other hand, it enables designing sparse arrays with performance similar to that of the fully populated dense arrays. In this paper we present a general systematic approach to the design and optimization of imaging systems based on the 2D array operating in the multistatic mode. We start from presenting principles of the SF schemes applied to LW imaging. Then, we outline the coarray concept and demonstrate how it can be used for reducing number of elements of the 2D arrays. Finally, efficient tools for the investigation and experimental verification of the designed 2D array prototypes are presented. The first step in the investigation is theoretical evaluation performed using frequency-dependent structure transfer function (STF), which enables approximate simulation of an array excited with a tone-burst in a dispersive medium. Finally, we show how scanning laser vibrometer, sensing waves in multiple points corresponding to the locations of the 2D receiving array elements, can be used as a tool for rapid experimental verification of the developed topologies. The presented methods are discussed in terms of the beampatterns and sparse versions of the fully populated array topologies are be presented. The effect of apodization applied to the array elements is also investigated. Both simulated and experimental results are included.

Rhodium-Catalyzed Asymmetric Conjugate Alkynylation/Aldol Cyclization Cascade for the Formation of α-Propargyl-β-hydroxyketones.

PubMed

Choo, Ken-Loon; Lautens, Mark

2018-03-02

A rhodium-catalyzed conjugate alkynylation/aldol cyclization cascade was developed. Densely functionalized cyclic α-propargyl-β-hydroxyketones were synthesized with simultaneous formation of a C(sp)-C(sp 3 ) bond, a C(sp 3 )-C(sp 3 ) bond, as well as three new contiguous stereocenters. The transformation was achieved with excellent enantio- and diastereoselectivities using BINAP as the ligand. The synthetic utility of the newly installed alkynyl moiety was exhibited by subjecting the products to an array of derivatizations.

Source and Path Calibration in Regions of Poor Crustal Propagation Using Temporary, Large-Aperture, High-Resolution Seismic Arrays (Postprint). Annual Report 3

DTIC Science & Technology

2012-06-04

central Tibetan Plateau. Automated hypocenter locations in south- central Tibet were finalized. Refinements included an update of the model used for... central Tibet. A subset of ~7,900 events with 25+ arrivals is considered well-located based on kilometer-scale differences relative to manually located...propagation in the Nepal Himalaya and the south- central Tibetan Plateau. The 2002–2005 experiment consisted of 233 stations along a dense 800 km linear

Fabrication and testing of polyimide-based microelectrode arrays for cortical mapping of evoked potentials.

PubMed

Myllymaa, Sami; Myllymaa, Katja; Korhonen, Hannu; Töyräs, Juha; Jääskeläinen, Juha E; Djupsund, Kaj; Tanila, Heikki; Lappalainen, Reijo

2009-06-15

Modern microfabrication techniques make it possible to develop microelectrode arrays that may be utilized not only in neurophysiological research but also in the clinic, e.g. in neurosurgery and as elements of neural prostheses. The aim of this study was to test whether a flexible microelectrode array is suitable for recording cortical surface field potentials in rats. Polyimide-based microelectrode arrays were fabricated by utilizing microfabrication techniques e.g. photolithography and magnetron sputter deposition. The present microelectrode array consists of eight platinum microelectrodes (round-shaped, Ø: 200 microm), transmission lines and connector pads sandwiched between two thin layers of biocompatible polyimide. The microelectrode arrays were electrochemically characterized by impedance spectroscopy in physiological saline solution and successfully tested in vivo by conducting acute and chronic measurements of evoked potentials on the surface of rat cortex. The arrays proved excellent flexibility and mechanical strength during handling and implantation onto the surface of cortex. The excellent electrochemical characteristics and stable in vivo recordings with high spatiotemporal resolution highlight the potential of these arrays. The fabrication protocol described here allows implementation of several other neural interfaces with different layouts, material selections or target areas either for recording or stimulation purposes.

Miniaturized neural interfaces and implants

NASA Astrophysics Data System (ADS)

Stieglitz, Thomas; Boretius, Tim; Ordonez, Juan; Hassler, Christina; Henle, Christian; Meier, Wolfgang; Plachta, Dennis T. T.; Schuettler, Martin

2012-03-01

Neural prostheses are technical systems that interface nerves to treat the symptoms of neurological diseases and to restore sensory of motor functions of the body. Success stories have been written with the cochlear implant to restore hearing, with spinal cord stimulators to treat chronic pain as well as urge incontinence, and with deep brain stimulators in patients suffering from Parkinson's disease. Highly complex neural implants for novel medical applications can be miniaturized either by means of precision mechanics technologies using known and established materials for electrodes, cables, and hermetic packages or by applying microsystems technologies. Examples for both approaches will be introduced and discussed. Electrode arrays for recording of electrocorticograms during presurgical epilepsy diagnosis have been manufactured using approved materials and a marking laser to achieve an integration density that is adequate in the context of brain machine interfaces, e.g. on the motor cortex. Microtechnologies have to be used for further miniaturization to develop polymer-based flexible and light weighted electrode arrays to interface the peripheral and central nervous system. Polyimide as substrate and insulation material will be discussed as well as several application examples for nerve interfaces like cuffs, filament like electrodes and large arrays for subdural implantation.

Distributed stimulation increases force elicited with functional electrical stimulation

NASA Astrophysics Data System (ADS)

Buckmire, Alie J.; Lockwood, Danielle R.; Doane, Cynthia J.; Fuglevand, Andrew J.

2018-04-01

Objective. The maximum muscle forces that can be evoked using functional electrical stimulation (FES) are relatively modest. The reason for this weakness is not fully understood but could be partly related to the widespread distribution of motor nerve branches within muscle. As such, a single stimulating electrode (as is conventionally used) may be incapable of activating the entire array of motor axons supplying a muscle. Therefore, the objective of this study was to determine whether stimulating a muscle with more than one source of current could boost force above that achievable with a single source. Approach. We compared the maximum isometric forces that could be evoked in the anterior deltoid of anesthetized monkeys using one or two intramuscular electrodes. We also evaluated whether temporally interleaved stimulation between two electrodes might reduce fatigue during prolonged activity compared to synchronized stimulation through two electrodes. Main results. We found that dual electrode stimulation consistently produced greater force (~50% greater on average) than maximal stimulation with single electrodes. No differences, however, were found in the fatigue responses using interleaved versus synchronized stimulation. Significance. It seems reasonable to consider using multi-electrode stimulation to augment the force-generating capacity of muscles and thereby increase the utility of FES systems.

Tinnitus treatment with precise and optimal electric stimulation: opportunities and challenges.

PubMed

Zeng, Fan-Gang; Djalilian, Hamid; Lin, Harrison

2015-10-01

Electric stimulation is a potent means of neuromodulation that has been used to restore hearing and minimize tremor, but its application on tinnitus symptoms has been limited. We examine recent evidence to identify the knowledge gaps in the use of electric stimulation for tinnitus treatment. Recent studies using electric stimulation to suppress tinnitus in humans are categorized according to their points of attacks. First, noninvasive, direct current stimulation uses an active electrode in the ear canal, tympanic membrane, or temporal scalp. Second, inner ear stimulation uses charge-balanced biphasic stimulation by placing an active electrode on the promontory or round window, or a cochlear implant array in the cochlea. Third, intraneural implants can provide targeted stimulation of specific sites along the auditory pathway. Although these studies demonstrated some success in tinnitus suppression, none established a link between tinnitus suppression efficacy and tinnitus-generating mechanisms. Electric stimulation provides a unique opportunity to suppress tinnitus. Challenges include matching electric stimulation sites and patterns to tinnitus locus and type, meeting the oftentimes-contradictory demands between tinnitus suppression and other indications, such as speech understanding, and justifying the costs and risks of electric stimulation for tinnitus symptoms.

Tinnitus Treatment with Precise and Optimal Electric Stimulation: Opportunities and Challenges

PubMed Central

Zeng, Fan-Gang; Djalilian, Hamid; Lin, Harrison

2015-01-01

Purpose of review Electric stimulation is a potent means of neuromodulation that has been used to restore hearing and minimize tremor, but its application on tinnitus symptoms has been limited. We examine recent evidence to identify the knowledge gaps in the use of electric stimulation for tinnitus treatment. Recent findings Recent studies using electric stimulation to suppress tinnitus in humans are categorized according to their points of attacks. First, non-invasive, direct-current stimulation uses an active electrode in the ear canal, tympanic membrane or temporal scalp. Second, inner ear stimulation uses charge-balanced biphasic stimulation by placing an active electrode on the promontory or round window, or a cochlear implant array in the cochlea. Third, intraneural implants can provide targeted stimulation of specific sites along the auditory pathway. Although these studies demonstrated some success in tinnitus suppression, none established a link between tinnitus suppression efficacy and tinnitus-generating mechanisms. Summary Electric stimulation provides a unique opportunity to suppress tinnitus. Challenges include matching electric stimulation sites and patterns to tinnitus locus and type, meeting the oftentimes-contradictory demands between tinnitus suppression and other indications, such as speech understanding, and justifying the costs and risks of electric stimulation for tinnitus symptoms. PMID:26208122

Paralyzed Patients Regain Voluntary Movement | NIH MedlinePlus the Magazine

MedlinePlus

... paralyzed. Now, thanks to a novel combination of electrical stimulation of their spinal cords and long-term ... array implanted on his spinal cord. It delivered electrical pulses just below his injury while he underwent ...

Loss of Huntingtin stimulates capture of retrograde dense-core vesicles to increase synaptic neuropeptide stores.

PubMed

Bulgari, Dinara; Deitcher, David L; Levitan, Edwin S

2017-08-01

The Huntington's disease protein Huntingtin (Htt) regulates axonal transport of dense-core vesicles (DCVs) containing neurotrophins and neuropeptides. DCVs travel down axons to reach nerve terminals where they are either captured in synaptic boutons to support later release or reverse direction to reenter the axon as part of vesicle circulation. Currently, the impact of Htt on DCV dynamics in the terminal is unknown. Here we report that knockout of Drosophila Htt selectively reduces retrograde DCV flux at proximal boutons of motoneuron terminals. However, initiation of retrograde transport at the most distal bouton and transport velocity are unaffected suggesting that synaptic capture rate of these retrograde DCVs could be altered. In fact, tracking DCVs shows that retrograde synaptic capture efficiency is significantly elevated by Htt knockout or knockdown. Furthermore, synaptic boutons contain more neuropeptide in Htt knockout larvae even though bouton size, single DCV fluorescence intensity, neuropeptide release in response to electrical stimulation and subsequent activity-dependent capture are unaffected. Thus, loss of Htt increases synaptic capture as DCVs travel by retrograde transport through boutons resulting in reduced transport toward the axon and increased neuropeptide in the terminal. These results therefore identify native Htt as a regulator of synaptic capture and neuropeptide storage. Copyright © 2017 Elsevier GmbH. All rights reserved.

Seismicity Induced by Water Injection for Geothermal Reservoir Stimulation 5 km Below the City of Basel, Switzerland

NASA Astrophysics Data System (ADS)

Deichmann, N.; Mai, M.; Bethmann, F.; Ernst, J.; Evans, K.; Fäh, D.; Giardini, D.; Häring, M.; Husen, S.; Kästli, P.; Bachmann, C.; Ripperger, J.; Schanz, U.; Wiemer, S.

2007-12-01

To stimulate the reservoir for a proposed "hot dry rock" geothermal project in the city of Basel, approximately 11500 m3 of water were injected between December 2nd and 8th, 2006, at high pressures into a 5 km deep well. A six-sensor borehole array, installed at depths between 300 and 2700 meters around the well to monitor the induced seismicity, recorded more than 10500 events during the injection phase. Events with magnitudes as low as ML 0.7 were also recorded by regional networks in Switzerland, Germany and France, as well as by up to 30 strong-motion stations installed in the epicentral area. Due to excessive seismic activity, that included an ML 2.7 event, injection had already been stopped when a few hours later an ML 3.4 event jolted the city of Basel. A large amount of (non-structural) damage, corresponding to an intensity of V (EMS98), has been claimed by home owners. The unusual number of damage claims compared to past events in this magnitude range observed in Switzerland is partly due to the shallow hypocenter (5 km) beneath a densely populated city. In addition, ground-motion modelling shows that the radiation pattern of the earthquake source was oriented unfavourably for the city of Basel and that in numerous locations shaking was amplified significantly by local site conditions. After bleed-off, about one third of the injected water volume flowed back out of the well. Although seismic activity declined rapidly thereafter, three additional ML>3 events occurred over the following two months. From a statistical analysis it is expected that it may take on the order of a decade for the activity to decrease to the regional background level scaled to the size of the source region. The hypocenters of the located events (about 3500 to date) are restricted to a NW-SE oriented lens-shaped cloud, about 1 km in diameter and 200 m wide, with a single offsetting branch to the ESE. The orientation of the cloud and the focal mechanisms determined so far match the stress field derived from observations of borehole breakouts and natural seismicity. Due to the premature abortion of the stimulation process, the size of the stimulated volume is insufficient for a commercially viable exploitation of geothermal energy. At present the project is on hold, pending a comprehensive assessment of the seismic risk associated with a continuation.

Electroencephalography: Subdural Multi-Electrode Brain Chip.

DTIC Science & Technology

1995-12-01

showing a blind subject reading Braille letters that had been inserted into his visual cortex by stimulating appropriate sets of electrodes. The...subject in Dobelle’s experiment 50 had been blind for 10 years and was able to read Braille at 30 letters a minute using a 64 electrode array for...Evans, "’ Braille ’ Reading by a Blind Volunteer by Visual cortex Stimulation," Nature, 259: 111-112 (January 1976). A.K. Engel, et al. "Temporal Coding

A fiber-based implantable multi-optrode array with contiguous optical and electrical sites

NASA Astrophysics Data System (ADS)

Chen, Sanyuan; Pei, Weihua; Gui, Qiang; Chen, Yuanfang; Zhao, Shanshan; Wang, Huan; Chen, Hongda

2013-08-01

Objective. Although various kinds of optrodes are designed to deliver light and sense electrophysiological responses, few have a tightly closed optical delivering site or electrical recording site. The large space between them often blurs the stimulation location and light intensity threshold. Approach. Based on an optical fiber, we develop an optrode structure which has a coniform tip where the light exit point and gold-based electrode site are located. The optrode is fabricated by integrating a metal membrane electrode on the outside of a tapered fiber. Half of the cone-shape tip is covered by a layer of gold membrane to form the electrode. A commercial fiber connector, mechanical transfer (MT) module, is chosen to assemble the multi-optrode array (MOA). The MT connector acts as both the holder of the optrode array and an aligning part to connect the MOA with the light source. Main results. We fabricated a pluggable MOA weighing only 0.2 g. The scanning electron microscope images showed a tight cover of the metal layer on the optrode tip with an exposure area of 1500 µm2. The electrochemical impedance of the optrode at 1 kHz was 100 kΩ on average and the light emission intensity reached 13 mW. The optical modulating and electrophysiological recording ability of the MOA was validated by monitoring the response of cells in a ChR2-expressing mouse's cerebral cortex. Neurons that maintained high cluster quality (signal-to-noise ratio = 5:1) and coherence in response to trains of 20 Hz stimulation were monitored. Significance. The optrode array reduces the distance between the optical stimulating sites and electrophysiological sites dramatically and can supply multiple channels to guide different lights simultaneously. This optrode with its novel structure may lead to a different kind of optical neural control prosthetic device, opening up a new option for neural modulation in the brain.

Polystyrene negative resist for high-resolution electron beam lithography

PubMed Central

2011-01-01

We studied the exposure behavior of low molecular weight polystyrene as a negative tone electron beam lithography (EBL) resist, with the goal of finding the ultimate achievable resolution. It demonstrated fairly well-defined patterning of a 20-nm period line array and a 15-nm period dot array, which are the densest patterns ever achieved using organic EBL resists. Such dense patterns can be achieved both at 20 and 5 keV beam energies using different developers. In addition to its ultra-high resolution capability, polystyrene is a simple and low-cost resist with easy process control and practically unlimited shelf life. It is also considerably more resistant to dry etching than PMMA. With a low sensitivity, it would find applications where negative resist is desired and throughput is not a major concern. PMID:21749679

Demonstration of arbitrary views based on autostereoscopic three-dimensional display system

NASA Astrophysics Data System (ADS)

Liu, Boyang; Sang, Xinzhu; Yu, Xunbo; Li, Liu; Yang, Le; Yan, Binbin; Wang, Kuiru; Yu, Chongxiu

2017-10-01

A method to realize arbitrary views for the lenticular lens array based on autostereoscopic three-dimensional display system is demonstrated. Normally, the number of views is proportional to pitch of the lenticular lens array. Increasing the number of views will result in reducing resolution and enhancing of granular sensation. 32 dense views can be achieved with one lenticular lens pitch covering 5.333 sub-pixels, which does significantly increases the number of views without affecting the resolution. But the structure of pitch and the number of views are fixed. Here, the 3D display method that the number of views can be changed artificially for most structures of lenticular lens is presented. Compared with the previous 32 views display method, the smoothness of motion parallex and the display depth of field are significantly improved.

Efficient energy absorption of intense ps-laser pulse into nanowire target

DOE Office of Scientific and Technical Information (OSTI.GOV)

Habara, H.; Honda, S.; Katayama, M.

The interaction between ultra-intense laser light and vertically aligned carbon nanotubes is investigated to demonstrate efficient laser-energy absorption in the ps laser-pulse regime. Results indicate a clear enhancement of the energy conversion from laser to energetic electrons and a simultaneously small plasma expansion on the surface of the target. A two-dimensional plasma particle calculation exhibits a high absorption through laser propagation deep into the nanotube array, even for a dense array whose structure is much smaller than the laser wavelength. The propagation leads to the radial expansion of plasma perpendicular to the nanotubes rather than to the front side. Thesemore » features may contribute to fast ignition in inertial confinement fusion and laser particle acceleration, both of which require high current and small surface plasma simultaneously.« less

Stimulated scattering of electromagnetic waves carrying orbital angular momentum in quantum plasmas.

PubMed

Shukla, P K; Eliasson, B; Stenflo, L

2012-07-01

We investigate stimulated scattering instabilities of coherent circularly polarized electromagnetic (CPEM) waves carrying orbital angular momentum (OAM) in dense quantum plasmas with degenerate electrons and nondegenerate ions. For this purpose, we employ the coupled equations for the CPEM wave vector potential and the driven (by the ponderomotive force of the CPEM waves) equations for the electron and ion plasma oscillations. The electrons are significantly affected by the quantum forces (viz., the quantum statistical pressure, the quantum Bohm potential, as well as the electron exchange and electron correlations due to electron spin), which are included in the framework of the quantum hydrodynamical description of the electrons. Furthermore, our investigation of the stimulated Brillouin instability of coherent CPEM waves uses the generalized ion momentum equation that includes strong ion coupling effects. The nonlinear equations for the coupled CPEM and quantum plasma waves are then analyzed to obtain nonlinear dispersion relations which exhibit stimulated Raman, stimulated Brillouin, and modulational instabilities of CPEM waves carrying OAM. The present results are useful for understanding the origin of scattered light off low-frequency density fluctuations in high-energy density plasmas where quantum effects are eminent.

Subwavelength micropillar array terahertz lasers.

PubMed

Krall, Michael; Brandstetter, Martin; Deutsch, Christoph; Detz, Hermann; Andrews, Aaron Maxwell; Schrenk, Werner; Strasser, Gottfried; Unterrainer, Karl

2014-01-13

We report on micropillar-based terahertz lasers with active pillars that are much smaller than the emission wavelength. These micropillar array lasers correspond to scaled-down band-edge photonic crystal lasers forming an active photonic metamaterial. In contrast to photonic crystal lasers which use significantly larger pillar structures, lasing emission is not observed close to high-symmetry points in the photonic band diagram, but in the effective medium regime. We measure stimulated emission at 4 THz for micropillar array lasers with pillar diameters of 5 µm. Our results not only demonstrate the integration of active subwavelength optics in a terahertz laser, but are also an important step towards the realization of nanowire-based terahertz lasers.

Accurate motor mapping in awake common marmosets using micro-electrocorticographical stimulation and stochastic threshold estimation

NASA Astrophysics Data System (ADS)

Kosugi, Akito; Takemi, Mitsuaki; Tia, Banty; Castagnola, Elisa; Ansaldo, Alberto; Sato, Kenta; Awiszus, Friedemann; Seki, Kazuhiko; Ricci, Davide; Fadiga, Luciano; Iriki, Atsushi; Ushiba, Junichi

2018-06-01

Objective. Motor map has been widely used as an indicator of motor skills and learning, cortical injury, plasticity, and functional recovery. Cortical stimulation mapping using epidural electrodes is recently adopted for animal studies. However, several technical limitations still remain. Test-retest reliability of epidural cortical stimulation (ECS) mapping has not been examined in detail. Many previous studies defined evoked movements and motor thresholds by visual inspection, and thus, lacked quantitative measurements. A reliable and quantitative motor map is important to elucidate the mechanisms of motor cortical reorganization. The objective of the current study was to perform reliable ECS mapping of motor representations based on the motor thresholds, which were stochastically estimated by motor evoked potentials and chronically implanted micro-electrocorticographical (µECoG) electrode arrays, in common marmosets. Approach. ECS was applied using the implanted µECoG electrode arrays in three adult common marmosets under awake conditions. Motor evoked potentials were recorded through electromyographical electrodes implanted in upper limb muscles. The motor threshold was calculated through a modified maximum likelihood threshold-hunting algorithm fitted with the recorded data from marmosets. Further, a computer simulation confirmed reliability of the algorithm. Main results. Computer simulation suggested that the modified maximum likelihood threshold-hunting algorithm enabled to estimate motor threshold with acceptable precision. In vivo ECS mapping showed high test-retest reliability with respect to the excitability and location of the cortical forelimb motor representations. Significance. Using implanted µECoG electrode arrays and a modified motor threshold-hunting algorithm, we were able to achieve reliable motor mapping in common marmosets with the ECS system.